LOCUS       DAZFZC010000001       123053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFZC010000001 DAZFZC010000000
VERSION     DAZFZC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 123053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 123053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..123053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.001"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(179..709)
                     /gene="cybB"
                     /locus_tag="ACHKXP_000001"
     CDS             complement(179..709)
                     /gene="cybB"
                     /locus_tag="ACHKXP_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO7645094.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIMPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            898..1899
                     /gene="gap"
                     /locus_tag="ACHKXP_000002"
                     /pseudo
     CDS             898..1899
                     /gene="gap"
                     /locus_tag="ACHKXP_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(1941..3380)
                     /gene="aldA"
                     /locus_tag="ACHKXP_000003"
     CDS             complement(1941..3380)
                     /gene="aldA"
                     /locus_tag="ACHKXP_000003"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO7645095.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3577..4377)
                     /gene="ydcF"
                     /locus_tag="ACHKXP_000004"
     CDS             complement(3577..4377)
                     /gene="ydcF"
                     /locus_tag="ACHKXP_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO7645096.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(4649..8551)
                     /gene="hrpA"
                     /locus_tag="ACHKXP_000005"
     CDS             complement(4649..8551)
                     /gene="hrpA"
                     /locus_tag="ACHKXP_000005"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO7645097.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            8752..9357
                     /gene="azoR"
                     /locus_tag="ACHKXP_000006"
     CDS             8752..9357
                     /gene="azoR"
                     /locus_tag="ACHKXP_000006"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO7645098.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVNFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(9408..10700)
                     /gene="ynbD"
                     /locus_tag="ACHKXP_000007"
     CDS             complement(9408..10700)
                     /gene="ynbD"
                     /locus_tag="ACHKXP_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO7645099.1"
                     /translation="MATRAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCGFLLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAERWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIKIALPYVVG
                     AGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(10713..12191)
                     /locus_tag="ACHKXP_000008"
                     /pseudo
     CDS             complement(10713..12191)
                     /locus_tag="ACHKXP_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
     gene            complement(12183..12377)
                     /locus_tag="ACHKXP_000009"
                     /pseudo
     CDS             complement(12183..12377)
                     /locus_tag="ACHKXP_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            complement(12548..14854)
                     /gene="ydbD"
                     /locus_tag="ACHKXP_000010"
     CDS             complement(12548..14854)
                     /gene="ydbD"
                     /locus_tag="ACHKXP_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO7645100.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANAVDIEQFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIIR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(14918..15778)
                     /gene="pdxI"
                     /locus_tag="ACHKXP_000011"
     CDS             complement(14918..15778)
                     /gene="pdxI"
                     /locus_tag="ACHKXP_000011"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO7645101.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLQLSEEV
                     LSTLDGISRE"
     gene            complement(16010..16600)
                     /gene="paaY"
                     /locus_tag="ACHKXP_000012"
     CDS             complement(16010..16600)
                     /gene="paaY"
                     /locus_tag="ACHKXP_000012"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO7645102.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREIEPGRKRLVFDENLRPKQ"
     gene            complement(16582..17532)
                     /gene="paaX"
                     /locus_tag="ACHKXP_000013"
     CDS             complement(16582..17532)
                     /gene="paaX"
                     /locus_tag="ACHKXP_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO7645103.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKTTLADVKKQLIWQGFGALAPSLMASPSQKLADVQALLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFIR"
     gene            complement(17633..18946)
                     /gene="paaK"
                     /locus_tag="ACHKXP_000014"
     CDS             complement(17633..18946)
                     /gene="paaK"
                     /locus_tag="ACHKXP_000014"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO7645104.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(18973..20178)
                     /gene="paaJ"
                     /locus_tag="ACHKXP_000015"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(18973..20178)
                     /gene="paaJ"
                     /locus_tag="ACHKXP_000015"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO7645105.1"
                     /translation="MREAFICDGIRTPIGRYGGALSSVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(20178..20600)
                     /gene="paaI"
                     /locus_tag="ACHKXP_000016"
     CDS             complement(20178..20600)
                     /gene="paaI"
                     /locus_tag="ACHKXP_000016"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO7645106.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(20590..22017)
                     /gene="paaH"
                     /locus_tag="ACHKXP_000017"
     CDS             complement(20590..22017)
                     /gene="paaH"
                     /locus_tag="ACHKXP_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO7645107.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(22019..22807)
                     /gene="paaG"
                     /locus_tag="ACHKXP_000018"
     CDS             complement(22019..22807)
                     /gene="paaG"
                     /locus_tag="ACHKXP_000018"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO7645108.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGGDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(22807..23574)
                     /gene="paaF"
                     /locus_tag="ACHKXP_000019"
     CDS             complement(22807..23574)
                     /gene="paaF"
                     /locus_tag="ACHKXP_000019"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO7645109.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(23571..24641)
                     /gene="paaE"
                     /locus_tag="ACHKXP_000020"
     CDS             complement(23571..24641)
                     /gene="paaE"
                     /locus_tag="ACHKXP_000020"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO7645110.1"
                     /translation="MTTFHSLTVAKVESETRDAVTITFAVHQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAETALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(24649..25146)
                     /gene="paaD"
                     /locus_tag="ACHKXP_000021"
     CDS             complement(24649..25146)
                     /gene="paaD"
                     /locus_tag="ACHKXP_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO7645111.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTNGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLREYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(25161..25907)
                     /gene="paaC"
                     /locus_tag="ACHKXP_000022"
     CDS             complement(25161..25907)
                     /gene="paaC"
                     /locus_tag="ACHKXP_000022"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO7645112.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(25916..26203)
                     /gene="paaB"
                     /locus_tag="ACHKXP_000023"
     CDS             complement(25916..26203)
                     /gene="paaB"
                     /locus_tag="ACHKXP_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO7645113.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(26215..27144)
                     /gene="paaA"
                     /locus_tag="ACHKXP_000024"
     CDS             complement(26215..27144)
                     /gene="paaA"
                     /locus_tag="ACHKXP_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO7645114.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLTWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            27429..29474
                     /gene="paaZ"
                     /locus_tag="ACHKXP_000025"
     CDS             27429..29474
                     /gene="paaZ"
                     /locus_tag="ACHKXP_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO7645115.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHLLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            29722..31995
                     /gene="tynA"
                     /locus_tag="ACHKXP_000026"
     CDS             29722..31995
                     /gene="tynA"
                     /locus_tag="ACHKXP_000026"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO7645116.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWFFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(32053..33552)
                     /gene="feaB"
                     /locus_tag="ACHKXP_000027"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(32053..33552)
                     /gene="feaB"
                     /locus_tag="ACHKXP_000027"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO7645117.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            33788..34693
                     /gene="feaR"
                     /locus_tag="ACHKXP_000028"
     CDS             33788..34693
                     /gene="feaR"
                     /locus_tag="ACHKXP_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO7645118.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(34865..35191)
                     /gene="ydbL"
                     /locus_tag="ACHKXP_000029"
     CDS             complement(34865..35191)
                     /gene="ydbL"
                     /locus_tag="ACHKXP_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO7645119.1"
                     /translation="MKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVADINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGING
                     KWVRKF"
     gene            complement(35199..35384)
                     /gene="ynbE"
                     /locus_tag="ACHKXP_000030"
     CDS             complement(35199..35384)
                     /gene="ynbE"
                     /locus_tag="ACHKXP_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO7645120.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(35381..38020)
                     /gene="ydbH"
                     /locus_tag="ACHKXP_000031"
     CDS             complement(35381..38020)
                     /gene="ydbH"
                     /locus_tag="ACHKXP_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO7645121.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFAMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPIVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGLDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHNPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            38228..39217
                     /gene="ldhA"
                     /locus_tag="ACHKXP_000032"
     CDS             38228..39217
                     /gene="ldhA"
                     /locus_tag="ACHKXP_000032"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7645122.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFEQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            39328..39750
                     /gene="hslJ"
                     /locus_tag="ACHKXP_000033"
     CDS             39328..39750
                     /gene="hslJ"
                     /locus_tag="ACHKXP_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO7645123.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(39747..40013)
                     /gene="ydbJ"
                     /locus_tag="ACHKXP_000034"
     CDS             complement(39747..40013)
                     /gene="ydbJ"
                     /locus_tag="ACHKXP_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO7645124.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            40287..43811
                     /gene="nifJ"
                     /locus_tag="ACHKXP_000035"
     CDS             40287..43811
                     /gene="nifJ"
                     /locus_tag="ACHKXP_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO7645125.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISQIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQVEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            44178..45311
                     /gene="ompN"
                     /locus_tag="ACHKXP_000036"
     CDS             44178..45311
                     /gene="ompN"
                     /locus_tag="ACHKXP_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO7645126.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            45452..45886
                     /gene="uspF"
                     /locus_tag="ACHKXP_000037"
     CDS             45452..45886
                     /gene="uspF"
                     /locus_tag="ACHKXP_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO7645127.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            46063..46998
                     /gene="ttcA"
                     /locus_tag="ACHKXP_000038"
     CDS             46063..46998
                     /gene="ttcA"
                     /locus_tag="ACHKXP_000038"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO7645128.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPACWQPEEDENQLDELRLNVVEVK"
     gene            complement(47127..48500)
                     /gene="dbpA"
                     /locus_tag="ACHKXP_000039"
     CDS             complement(47127..48500)
                     /gene="dbpA"
                     /locus_tag="ACHKXP_000039"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO7645129.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIATLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(48530..48703)
                     /gene="ynaL"
                     /locus_tag="ACHKXP_000040"
     CDS             complement(48530..48703)
                     /gene="ynaL"
                     /locus_tag="ACHKXP_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO7645130.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(48978..49961)
                     /gene="zntB"
                     /locus_tag="ACHKXP_000041"
     CDS             complement(48978..49961)
                     /gene="zntB"
                     /locus_tag="ACHKXP_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO7645131.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(49975..50022)
                     /gene="ydaN"
                     /locus_tag="ACHKXP_000042"
     CDS             complement(49975..50022)
                     /gene="ydaN"
                     /locus_tag="ACHKXP_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO7645132.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            50216..51448
                     /gene="dgcM"
                     /locus_tag="ACHKXP_000043"
     CDS             50216..51448
                     /gene="dgcM"
                     /locus_tag="ACHKXP_000043"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO7645133.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAAKLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(51469..52032)
                     /gene="smrA"
                     /locus_tag="ACHKXP_000044"
     CDS             complement(51469..52032)
                     /gene="smrA"
                     /locus_tag="ACHKXP_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO7645134.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(52362..53270)
                     /gene="abgR"
                     /locus_tag="ACHKXP_000045"
     CDS             complement(52362..53270)
                     /gene="abgR"
                     /locus_tag="ACHKXP_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7645135.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            53446..54756
                     /gene="abgA"
                     /locus_tag="ACHKXP_000046"
     CDS             53446..54756
                     /gene="abgA"
                     /locus_tag="ACHKXP_000046"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO7645136.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            54756..56201
                     /gene="abgB"
                     /locus_tag="ACHKXP_000047"
     CDS             54756..56201
                     /gene="abgB"
                     /locus_tag="ACHKXP_000047"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO7645137.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPISATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYRALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            56235..57761
                     /gene="abgT"
                     /locus_tag="ACHKXP_000048"
     CDS             56235..57761
                     /gene="abgT"
                     /locus_tag="ACHKXP_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO7645138.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            57772..58287
                     /gene="ogt"
                     /locus_tag="ACHKXP_000049"
     CDS             57772..58287
                     /gene="ogt"
                     /locus_tag="ACHKXP_000049"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO7645139.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            58482..59234
                     /gene="fnr"
                     /locus_tag="ACHKXP_000050"
     CDS             58482..59234
                     /gene="fnr"
                     /locus_tag="ACHKXP_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO7645140.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            59386..60336
                     /gene="uspE"
                     /locus_tag="ACHKXP_000051"
     CDS             59386..60336
                     /gene="uspE"
                     /locus_tag="ACHKXP_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO7645141.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(60386..60643)
                     /gene="ynaJ"
                     /locus_tag="ACHKXP_000052"
     CDS             complement(60386..60643)
                     /gene="ynaJ"
                     /locus_tag="ACHKXP_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO7645142.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            60887..61918
                     /gene="ynaI"
                     /locus_tag="ACHKXP_000053"
     CDS             60887..61918
                     /gene="ynaI"
                     /locus_tag="ACHKXP_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO7645143.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(61969..63582)
                     /gene="mppA"
                     /locus_tag="ACHKXP_000054"
     CDS             complement(61969..63582)
                     /gene="mppA"
                     /locus_tag="ACHKXP_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO7645144.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESVATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(63919..64817)
                     /gene="pgrR"
                     /locus_tag="ACHKXP_000055"
                     /pseudo
     CDS             complement(63919..64817)
                     /gene="pgrR"
                     /locus_tag="ACHKXP_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
     gene            64955..65875
                     /gene="ycjY"
                     /locus_tag="ACHKXP_000056"
     CDS             64955..65875
                     /gene="ycjY"
                     /locus_tag="ACHKXP_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7645145.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            65946..66083
                     /locus_tag="ACHKXP_000057"
                     /pseudo
     CDS             65946..66083
                     /locus_tag="ACHKXP_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            66210..66938
                     /gene="mpaA"
                     /locus_tag="ACHKXP_000058"
     CDS             66210..66938
                     /gene="mpaA"
                     /locus_tag="ACHKXP_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO7645146.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(66913..67878)
                     /gene="ycjG"
                     /locus_tag="ACHKXP_000059"
     CDS             complement(66913..67878)
                     /gene="ycjG"
                     /locus_tag="ACHKXP_000059"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO7645147.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLVARR
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            67997..68503
                     /gene="tpx"
                     /locus_tag="ACHKXP_000060"
     CDS             67997..68503
                     /gene="tpx"
                     /locus_tag="ACHKXP_000060"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO7645148.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(68547..70088)
                     /gene="tyrR"
                     /locus_tag="ACHKXP_000061"
     CDS             complement(68547..70088)
                     /gene="tyrR"
                     /locus_tag="ACHKXP_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO7645149.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(70236..71297)
                     /gene="ycjF"
                     /locus_tag="ACHKXP_000062"
     CDS             complement(70236..71297)
                     /gene="ycjF"
                     /locus_tag="ACHKXP_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO7645150.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(71294..72691)
                     /gene="ycjX"
                     /locus_tag="ACHKXP_000063"
     CDS             complement(71294..72691)
                     /gene="ycjX"
                     /locus_tag="ACHKXP_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO7645151.1"
                     /translation="MKRLKNELNALLNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMSEGLDPLAPADENRLADIAAAWTDYLHHCKEQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            72847..73845
                     /gene="ycjW"
                     /locus_tag="ACHKXP_000064"
     CDS             72847..73845
                     /gene="ycjW"
                     /locus_tag="ACHKXP_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7645152.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFCDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALKAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(73956..74861)
                     /gene="ompG"
                     /locus_tag="ACHKXP_000065"
     CDS             complement(73956..74861)
                     /gene="ompG"
                     /locus_tag="ACHKXP_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO7645153.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(74906..75988)
                     /locus_tag="ACHKXP_000066"
     CDS             complement(74906..75988)
                     /locus_tag="ACHKXP_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7645154.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVSGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KHIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(76002..76661)
                     /gene="pgmB"
                     /locus_tag="ACHKXP_000067"
     CDS             complement(76002..76661)
                     /gene="pgmB"
                     /locus_tag="ACHKXP_000067"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO7645155.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(76658..78925)
                     /gene="ycjT"
                     /locus_tag="ACHKXP_000068"
     CDS             complement(76658..78925)
                     /gene="ycjT"
                     /locus_tag="ACHKXP_000068"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO7645156.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGSGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGSYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETMTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAYTSYMARYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELWMPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLITVAEESVFCLGDF
                     ILPFNGTATKHQEDE"
     gene            complement(78922..79977)
                     /gene="ycjS"
                     /locus_tag="ACHKXP_000069"
     CDS             complement(78922..79977)
                     /gene="ycjS"
                     /locus_tag="ACHKXP_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7645157.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(79987..80775)
                     /gene="ycjR"
                     /locus_tag="ACHKXP_000070"
     CDS             complement(79987..80775)
                     /gene="ycjR"
                     /locus_tag="ACHKXP_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7645158.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(80794..81844)
                     /gene="ycjQ"
                     /locus_tag="ACHKXP_000071"
                     /pseudo
     CDS             complement(80794..81844)
                     /gene="ycjQ"
                     /locus_tag="ACHKXP_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(81875..82717)
                     /gene="ycjP"
                     /locus_tag="ACHKXP_000072"
     CDS             complement(81875..82717)
                     /gene="ycjP"
                     /locus_tag="ACHKXP_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7645159.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVAALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(82704..83585)
                     /gene="ycjO"
                     /locus_tag="ACHKXP_000073"
     CDS             complement(82704..83585)
                     /gene="ycjO"
                     /locus_tag="ACHKXP_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7645160.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(83606..84898)
                     /gene="ycjN"
                     /locus_tag="ACHKXP_000074"
     CDS             complement(83606..84898)
                     /gene="ycjN"
                     /locus_tag="ACHKXP_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7645161.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVRDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(84912..86591)
                     /gene="ycjM"
                     /locus_tag="ACHKXP_000075"
     CDS             complement(84912..86591)
                     /gene="ycjM"
                     /locus_tag="ACHKXP_000075"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO7645162.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(86804..87149)
                     /gene="pspE"
                     /locus_tag="ACHKXP_000076"
                     /pseudo
     CDS             complement(86804..87149)
                     /gene="pspE"
                     /locus_tag="ACHKXP_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
     gene            complement(87192..87413)
                     /gene="pspD"
                     /locus_tag="ACHKXP_000077"
     CDS             complement(87192..87413)
                     /gene="pspD"
                     /locus_tag="ACHKXP_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO7645163.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(87422..87781)
                     /gene="pspC"
                     /locus_tag="ACHKXP_000078"
     CDS             complement(87422..87781)
                     /gene="pspC"
                     /locus_tag="ACHKXP_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO7645164.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(87781..88005)
                     /gene="pspB"
                     /locus_tag="ACHKXP_000079"
     CDS             complement(87781..88005)
                     /gene="pspB"
                     /locus_tag="ACHKXP_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO7645165.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(88059..88727)
                     /gene="pspA"
                     /locus_tag="ACHKXP_000080"
     CDS             complement(88059..88727)
                     /gene="pspA"
                     /locus_tag="ACHKXP_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO7645166.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            88894..89871
                     /gene="pspF"
                     /locus_tag="ACHKXP_000081"
     CDS             88894..89871
                     /gene="pspF"
                     /locus_tag="ACHKXP_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO7645167.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(89991..91257)
                     /gene="puuE"
                     /locus_tag="ACHKXP_000082"
                     /pseudo
     CDS             complement(89991..91257)
                     /gene="puuE"
                     /locus_tag="ACHKXP_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(91295..92575)
                     /gene="puuB"
                     /locus_tag="ACHKXP_000083"
     CDS             complement(91295..92575)
                     /gene="puuB"
                     /locus_tag="ACHKXP_000083"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO7645168.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(92577..94064)
                     /gene="puuC"
                     /locus_tag="ACHKXP_000084"
     CDS             complement(92577..94064)
                     /gene="puuC"
                     /locus_tag="ACHKXP_000084"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO7645169.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(94339..94896)
                     /gene="puuR"
                     /locus_tag="ACHKXP_000085"
     CDS             complement(94339..94896)
                     /gene="puuR"
                     /locus_tag="ACHKXP_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO7645170.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(94923..95687)
                     /gene="puuD"
                     /locus_tag="ACHKXP_000086"
     CDS             complement(94923..95687)
                     /gene="puuD"
                     /locus_tag="ACHKXP_000086"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO7645171.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALS
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            95899..97316
                     /gene="puuA"
                     /locus_tag="ACHKXP_000087"
                     /pseudo
     CDS             95899..97316
                     /gene="puuA"
                     /locus_tag="ACHKXP_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
     gene            97465..97629
                     /locus_tag="ACHKXP_000088"
     CDS             97465..97629
                     /locus_tag="ACHKXP_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645172.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            97619..99004
                     /gene="puuP"
                     /locus_tag="ACHKXP_000089"
     CDS             97619..99004
                     /gene="puuP"
                     /locus_tag="ACHKXP_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO7645173.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            99138..99383
                     /gene="ymjA"
                     /locus_tag="ACHKXP_000090"
     CDS             99138..99383
                     /gene="ymjA"
                     /locus_tag="ACHKXP_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO7645174.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINLVRIDEIAEFLNQWGNE"
     gene            99696..101339
                     /gene="sapA"
                     /locus_tag="ACHKXP_000091"
     CDS             99696..101339
                     /gene="sapA"
                     /locus_tag="ACHKXP_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO7645175.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            101336..102301
                     /gene="sapB"
                     /locus_tag="ACHKXP_000092"
     CDS             101336..102301
                     /gene="sapB"
                     /locus_tag="ACHKXP_000092"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO7645176.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            102288..103178
                     /gene="sapC"
                     /locus_tag="ACHKXP_000093"
     CDS             102288..103178
                     /gene="sapC"
                     /locus_tag="ACHKXP_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO7645177.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            103178..104170
                     /gene="sapD"
                     /locus_tag="ACHKXP_000094"
     CDS             103178..104170
                     /gene="sapD"
                     /locus_tag="ACHKXP_000094"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO7645178.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            104172..104978
                     /gene="sapF"
                     /locus_tag="ACHKXP_000095"
     CDS             104172..104978
                     /gene="sapF"
                     /locus_tag="ACHKXP_000095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO7645179.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            105046..105399
                     /gene="ycjD"
                     /locus_tag="ACHKXP_000096"
     CDS             105046..105399
                     /gene="ycjD"
                     /locus_tag="ACHKXP_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO7645180.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTNWLESQGWTVLRFWNNEIDCNEVAV
                     LEIILQELNRRSPSP"
     gene            105769..106557
                     /gene="fabI"
                     /locus_tag="ACHKXP_000097"
     CDS             105769..106557
                     /gene="fabI"
                     /locus_tag="ACHKXP_000097"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO7645181.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            106701..107828
                     /gene="yciW"
                     /locus_tag="ACHKXP_000098"
     CDS             106701..107828
                     /gene="yciW"
                     /locus_tag="ACHKXP_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO7645182.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSMESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASQFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            107896..109830
                     /gene="rnb"
                     /locus_tag="ACHKXP_000099"
     CDS             107896..109830
                     /gene="rnb"
                     /locus_tag="ACHKXP_000099"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO7645183.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(109908..110012)
                     /locus_tag="ACHKXP_000100"
     CDS             complement(109908..110012)
                     /locus_tag="ACHKXP_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645184.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            110066..112051
                     /gene="pdeR"
                     /locus_tag="ACHKXP_000101"
     CDS             110066..112051
                     /gene="pdeR"
                     /locus_tag="ACHKXP_000101"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7645185.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(112033..112161)
                     /gene="ymiD"
                     /locus_tag="ACHKXP_000102"
     CDS             complement(112033..112161)
                     /gene="ymiD"
                     /locus_tag="ACHKXP_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO7645186.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            112199..112372
                     /gene="yciZ"
                     /locus_tag="ACHKXP_000103"
     CDS             112199..112372
                     /gene="yciZ"
                     /locus_tag="ACHKXP_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO7645187.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            112462..113211
                     /gene="yciT"
                     /locus_tag="ACHKXP_000104"
     CDS             112462..113211
                     /gene="yciT"
                     /locus_tag="ACHKXP_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO7645188.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            113480..113698
                     /gene="osmB"
                     /locus_tag="ACHKXP_000105"
     CDS             113480..113698
                     /gene="osmB"
                     /locus_tag="ACHKXP_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO7645189.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(113824..114150)
                     /gene="yciH"
                     /locus_tag="ACHKXP_000106"
     CDS             complement(113824..114150)
                     /gene="yciH"
                     /locus_tag="ACHKXP_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO7645190.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(114150..114887)
                     /gene="pyrF"
                     /locus_tag="ACHKXP_000107"
     CDS             complement(114150..114887)
                     /gene="pyrF"
                     /locus_tag="ACHKXP_000107"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO7645191.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(115081..116250)
                     /gene="lapB"
                     /locus_tag="ACHKXP_000108"
     CDS             complement(115081..116250)
                     /gene="lapB"
                     /locus_tag="ACHKXP_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO7645192.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(116257..116565)
                     /gene="lapA"
                     /locus_tag="ACHKXP_000109"
     CDS             complement(116257..116565)
                     /gene="lapA"
                     /locus_tag="ACHKXP_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO7645193.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(116714..117478)
                     /gene="pgpB"
                     /locus_tag="ACHKXP_000110"
     CDS             complement(116714..117478)
                     /gene="pgpB"
                     /locus_tag="ACHKXP_000110"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO7645194.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            117648..118238
                     /gene="ribA"
                     /locus_tag="ACHKXP_000111"
     CDS             117648..118238
                     /gene="ribA"
                     /locus_tag="ACHKXP_000111"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO7645195.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(118302..120977)
                     /gene="acnA"
                     /locus_tag="ACHKXP_000112"
     CDS             complement(118302..120977)
                     /gene="acnA"
                     /locus_tag="ACHKXP_000112"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO7645196.1"
                     /translation="MSSTLREASKDALQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKGIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(121141..121236)
                     /gene="ymiC"
                     /locus_tag="ACHKXP_000113"
     CDS             complement(121141..121236)
                     /gene="ymiC"
                     /locus_tag="ACHKXP_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO7645197.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(121350..121517)
                     /gene="yciX"
                     /locus_tag="ACHKXP_000114"
     CDS             complement(121350..121517)
                     /gene="yciX"
                     /locus_tag="ACHKXP_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO7645198.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(121520..121660)
                     /gene="ymiA"
                     /locus_tag="ACHKXP_000115"
     CDS             complement(121520..121660)
                     /gene="ymiA"
                     /locus_tag="ACHKXP_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO7645199.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(121979..122953)
                     /gene="cysB"
                     /locus_tag="ACHKXP_000116"
     CDS             complement(121979..122953)
                     /gene="cysB"
                     /locus_tag="ACHKXP_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO7645200.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT        30222 a        32304 c        30019 g        30508 t
ORIGIN      
        1 cgttgatgag atcaaagatc gggtgaattg attaaaaata agccagatgg acgtatgggc
       61 aaggattatg gttttattta tcatacaaat aaatataata ggcgcttccg atgtagaccc
      121 gtattcttcg cctgtaccac gggtcggttt tagtacaggc gttttcttta aatatccttc
      181 aggaacgttt acgcggcatc atgcgtagaa gtgtgttgtc cttccagaaa tagtggtgtg
      241 ccagtgcagc cgcagcgtgc aacccgatga caaaatatcc cagattcgcc agcgtttcat
      301 gccacgactt taagctatct acccgttcga aattggcctc tgaagcgtaa ggcatcgtca
      361 aaccaaacgc aaaccacggg ttgccccggt tatacatcat caccaaacca atcactggca
      421 gcgcaataaa caggagataa atcaccaaat gtcccaaatg cgccagtccc gtcatcatcg
      481 gttttggctt aggcataatc ggcggggttg ggtatttcag ccttaacaaa agacgaacga
      541 ccatcagcac gagaattgag atgccacagg aaacatgaat catgttgatg agtggccgat
      601 cgctacgtgg gaagaaacca cgaaactcca ttgcgcaata cgctgcgata accagtaaaa
      661 agaccagcca gtgaatgctg atttgtaacc ttgaatattt attttccata acatttcctg
      721 ctttaacata attttccgtt aacataacgg gcttttctca aaatttcatt aaatattgtt
      781 cacccgtttt cagtacgtta cgtttcactg tgagaatgga gattgcccat cccgccatcc
      841 tggtctaagc ctggaaagga tcaattttca tccgaacgtt cctgacagga gaaaaccatg
      901 agtaaagttg gtattaacgg ttttggtcgt atcggtcgac tggtgttggg tcgattactt
      961 gaagtcaaaa gcaacataga cgttgtcgct attaatgatc tcacttcccc ataaattctc
     1021 gcctacctgc tgaaacatga ttcaaactac ggaccattcc cctggagcgt tgattttacg
     1081 gaagattcac ttatcgttga tgggaaaagt atcgcggttt acgccgaaaa agaggctaaa
     1141 aatattccgt ggaaagcgaa aggtgcagaa atcattgtcg aatgtactgg cttttatacc
     1201 tccgccgaga aatcgcaggc gcatcttgat gctggtgcga agaaggtgtt gatttccgcc
     1261 cctgccggtg aaatgaaaac tatcgtttat aacgtcaatg acgacactct ggatggcaac
     1321 gacaccattg tttccgtggc gtcatgcacc actaactgtc ttgcgccgat ggccaaagcc
     1381 ttgcatgaca gtttcgggat agaagtcggc acgatgacga ccattcatgc ctatactggc
     1441 acccagtcac tggtggatgg cccacgtggt aaagatttac gtgcttcacg cgcagcggca
     1501 gaaaatatca ttccccacac tacgggggcg gcaaaagcca ttggtctggt gatcccggaa
     1561 ctgagcggca aactgaaagg tcatgcgcaa cgcgtgccgg tgaaaacagg ttcggtcact
     1621 gagctggtgt ccattctcgg aaaaaaagtg actgccgaag aggtgaataa cgcacttaaa
     1681 caagcaacca ccaataacga gtcatttggt tataccgatg aagaaatagt ctcttccgat
     1741 atcattggca gccatttcgg ttcggtgttt gatgccacgc aaacggaaat taccgccgtg
     1801 ggcgatttac aactggtgaa aacggtcgcc tggtacgata acgaatatgg cttcgtcacg
     1861 cagcttattc gcaccctcga aaaattcgct aaactctgac gcgcacaggc ggaggaaaaa
     1921 acctccgcct cttttactca ttacgactgt aaataaacca cctgggtctg cagatattca
     1981 tgcaggccat gtttaccatc tgcgccgcca ataccggatt tacgccatcc ggcgtggaag
     2041 ccttgcatag cttcgaagtt ttcacggttg atgtaagttt caccaaactt cagcccttta
     2101 atggctttca tcgcgacgtt cagattttgg gtatagattg atgaggtcag gccgtaatca
     2161 ctgtcattag ccattgagat agcctcttcc agcgtgtcaa atgcgaccac cggcagaacc
     2221 gggccaaagg tttcctcatg cataatcgac atttcctggc gaacatccag cagcaatgtc
     2281 ggcggataat aatatccttt cccctctacc gctttgccac ccaacgccac tctcgcccct
     2341 tcttctactg cgcgcgccac tttttgctca accctttcca gcgccgcggc gttaatcaac
     2401 ggccccatcg caatgtcgtt gcgttcagcg gggttaccaa attgaaccgc ctgcatcgct
     2461 tcacccagcc gattgacgaa ctgatcataa atgcctttct gtacataaat acgttctgca
     2521 cagttacaca cttgcccact attaatgacg cgtgaatcaa cgatggcttt gactgccagt
     2581 tcaagatcgg catcgtccat tacgatagct ggtgctttac cccccaattc cagacacact
     2641 ttggtgatgt ttttcgccgc agtcgccatg atcttctcac ctgcagagac gctgcctgtc
     2701 atactgacca ttgcgacctt tgggttaccc gccagttctt gcccaacggt ttcaccacgc
     2761 cccagtacaa ggttaaacac gccgcgcgga aggcctattt catcgacgat tttggcgaat
     2821 gcaatcgcat tgtttggcgt aaattcacta ggtttaatga cgatggtatt accggtcaaa
     2881 agagcgggag ccattttgcg ggcaatgagg aagaacggga agttccacgg cagaatgccg
     2941 gtagtcacac caagcgcacg tttaaacaaa agaatatttt ctcctggacg atcgctttga
     3001 ataatctcgc cctcgtaacg ccgtgcccac tccgccatgt aatcgatata gtcggcagta
     3061 aaagccactt cgacttcagc cagctgctgg atcttgcccc cttcttcaac aatcagcgca
     3121 ctgatttcac tggcgcgttc gcggatcccg gcggagattt tgcgcaacca actggcgcgt
     3181 tcaatagcag gcaacgcttc ccattctggt tgtgcacgtt ctgctgcatc gattgcctta
     3241 cgggcatcct cggcctgacc atcgggtatg cgggaaatga cagcctctgt agcagggttt
     3301 accacatcaa tccatgcgtc tccacgccag gtaacaaact gtccatcgat atacatagga
     3361 tgttgaacgg gtactgacat gggcgactcc tgtgatttat atgtttgttt tcggtgaatg
     3421 cattgttaat taacatcaat catcagagag acggcatcgt gaaaggacaa ttctgtgaag
     3481 ggcttcataa aaacaagtgt aaaaggaata aaaggcagaa ataaaagtca ggagtcatgc
     3541 ccgtttgcaa acaagtggtt cacaaacggg caatttttaa cgtaacgagc gactttccat
     3601 cgcctcgatg agcaccgcat catgtttcag cgtttgccat gcatggatga cttctgccgg
     3661 aaaatcaacg tgaacgataa aatctcgccc gcggggaccg tagccatcgc tatcatcgcg
     3721 taaacgcggc agctcgccag tgagtagtga gagataacgc tcaactggcc ataatccttg
     3781 caacgggtta acaaagatta cactgtctgc gttatttcct aactgaggaa cgaatccggg
     3841 ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga acgtcgccat
     3901 cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc gttctacggc
     3961 ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg actggtcttc
     4021 aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg ccaggatggt
     4081 cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg cgatggcgct
     4141 atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa tttgctgatc
     4201 gcgagcaatc ttacatgccg catcgatagt cggcataacc gcatttcctg caagtatgac
     4261 gcaatcagcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc actgtccgat
     4321 aacatttatg gcatctatag ttgccgtcga aagcgtcgga aacggggtaa tgttcatggt
     4381 tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc ggagcactct
     4441 gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata tcttttaagg
     4501 aaatgctgta gataaacatt attcatcttg cagctgagaa ttgtattttt ctgaattcct
     4561 gaatttttgt ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat gcttaccggg
     4621 ttttagattt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc gcctgcaaaa
     4681 tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa ctaacgcgca
     4741 gctcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt gcaggcggca
     4801 gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc atctgcgcac
     4861 ggtcgcgatg tggatcaacc gccagttttt ccagtcgttt ttcaatcgcc tgcaaatatc
     4921 gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga tataccaacc
     4981 cgcccatctg cgctttaatg tcagaaagcc ccagcgccat ggtcatatcc acccgccctt
     5041 tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc ttcgcaatat
     5101 ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca aagccttctt
     5161 ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag gagatacagt
     5221 cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc ttggctttgt
     5281 ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc agtagacggc
     5341 gaagaccgtt ccacattgcc tgtttttgct ctagcggatt atcaaacagt ttgatcgcca
     5401 cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg ccacgcttct
     5461 gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagcccgctc tgctcgatac
     5521 cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca tctttcagat
     5581 cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg cggaaggtga
     5641 ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg gtaacgccgg
     5701 tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc ggtgtgacgc
     5761 ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt accggtttcg
     5821 gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc tgccattcaa
     5881 acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc acaccgtccg
     5941 cgtctgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc tgatgccaga
     6001 agttcgggta atccagcttg ctgatttttt ccgccccttc tttgatcaac atgctctttt
     6061 caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta tcgaagtgac
     6121 gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacaac gtttcgtcat
     6181 caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg gcccgcagtt
     6241 tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc agcgcatggc
     6301 gaataaagag ttcacgacat aacgccggat cgatctggct gtagttgacc ttgcgcgcgg
     6361 caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg ccctgcgccc
     6421 gttcccagtg cggttcgctg taggtacgtt taatcaaatg ctgagcgaca ggctccaccc
     6481 attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct accagttccg
     6541 ccaccattac ccatttcggc ggttttttaa ataaaccaga accggggaag atggagaaac
     6601 gcgcgttacg tgcgccggta tattcttgtt tatcggcatc tttcatgccg atatgggaaa
     6661 gtaaacccgt cagcaacgca atgtgaattt cgcgatactc cgccggttcg ctgttaaccg
     6721 gaatgccaag ttctttcacc acctgacgca actgggtgta gatatcctgc cattcgcgca
     6781 cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg gaagaaagcg
     6841 ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag tcggattctt
     6901 tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc ggacgttcgc
     6961 gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg cagccatgtt
     7021 tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc gagagctggc
     7081 gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc gcgcccagtt
     7141 cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg acaaacggga
     7201 acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag gccaggttgg
     7261 tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct tcggaataga
     7321 gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga ttggcagacg
     7381 cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg tagcggctga
     7441 tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc gaggtttccg
     7501 cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg cggttctgtt
     7561 cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag ttcagcttgt
     7621 tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc agaatgtcgc
     7681 cagggctttc ctggctcagt tcgtctacgg cgtcaaaaat cgcctgcaac tgatcgcgct
     7741 cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga taggtccgac
     7801 cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc gggtcgatag
     7861 tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct ttcaaatagc
     7921 cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg atggtgtcgt
     7981 actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg gtcatcagct
     8041 tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg atgcaaccgc
     8101 ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc gccaggcgac
     8161 gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata cagattttcg
     8221 gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc tggtgatcac
     8281 gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca ggataagtaa
     8341 tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca atctctttcg
     8401 ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttctta acaccgtgca
     8461 gacggcgaga aaaacgcagt ctgtcgcgca gcatcagcga atccagccgc tgctgcaagg
     8521 ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtaat ctgcctgatt
     8581 aattttcttt taaatgatag atatagagta ccacatcgcg gctttctatg cgttgttcaa
     8641 taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt gtgaataaag
     8701 tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac catgagcaag
     8761 gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca gttgtccgat
     8821 tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt tcgcgacctg
     8881 gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg tccgagcgat
     8941 gcgccgctga ctcctcgtca gcaagaagct ctggcgcttt ccgatgagtt gattgccgag
     9001 ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa tatctcgact
     9061 cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg ctataccgag
     9121 aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag ccgcggcggg
     9181 atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt cctcggcttt
     9241 atcggcatta ccgatgtgaa ttttgtcttc gccgaaggga tcgcatacgg tccggaaatg
     9301 gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc tgcataaccg
     9361 gacacgatct tgatgtggac tgtagttcag tccacatctc aatccactta cctgttttcc
     9421 cataatttca acatcgcttt gtgctcttcc ttaagcacaa tatgcgagcg tctggctcga
     9481 ataaagctaa tcgcttcatc aacggtttta cagtgtccgt aacataacag ccatgccgcc
     9541 actaccagcg cactgcgcga taatcccaat gcgcaatgga ccagaacgct gccttgctct
     9601 tcgcgtaatg tttccagcat cgccacggcc tgtcgtagct ccccctcttc cggaaccacc
     9661 agatccagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc gcgagggaat
     9721 tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa cgcccctaaa
     9781 taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca acgcatagac
     9841 agccacatcc cgatgcggca gggcaatgtc agccagtaaa cggcgggcgg tagtttcccc
     9901 tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc aattatgagt
     9961 agcgataata ctggccaaca taaccagact gaccaccata actgaatcat catcattagc
    10021 tccatcaata caatgcacga gcccgcgcct acgacatagg gcagtgctat tttgattcga
    10081 cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca gtcaatcaac
    10141 atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccaggt cgtcagcgtc
    10201 gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctctc agccagatgc
    10261 tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg agactggtta
    10321 taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc actcacttca
    10381 ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc cattaccgtt
    10441 gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca aacgaacagc
    10501 gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg aagaaaaggg
    10561 atcgccgttt cccaaccgaa tacctgactg gggatatcat ggctgttaag gtcctgaacc
    10621 gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag caacaataac
    10681 cagccagccc ttgtagcaat acgttccgac gttcagttat cacgacgcac cgccatcgaa
    10741 accgtaaaaa taccccactc atcaatccgt tgtgtgcatt tatcaaatcc ggcatcacgc
    10801 acgagtgaat ccatctcccc ttgcgaacgc acgcgcatta cccacggttt accatcttta
    10861 tgactggtta acaccccggc aatcatctcc agttgagggt gccacggctg cccggtgtag
    10921 atgagaatgc cgcccggttc gatggcattg gcaagacctg cgagtgagtt ttttacctgc
    10981 tcattttcgg gaaaaagctc atacaggcca gagacaatcg ccagcgtagg ccgcggagtt
    11041 aacgcgctga gttcctccgg gttaaacgca tcgccctgtt caaaacgcac ccgcccagac
    11101 attccccgtt gagcaatcat ctcttgcccc tgtgcaacat ttaactcgct gtaatcacgt
    11161 aacaaaatat cgcttacggc aggctcgttt gccagcgcat ccagtacata gcgcccatgc
    11221 cctgcggcaa tgtcaaccac gcggacggct aaacctttgg cgtggagatc ggcaacggct
    11281 tgtttaatca gtatttgtaa atgggttttg cgctggcgaa taccgcgcca gccaacactg
    11341 ttcaggtaga ttttgtcgac taaacgcccg aatgcgttac taccttgcgg ttgattttga
    11401 tagacataat ccagcgaact gcctgaatca aagccggtgc tcattccgag atgcaggccc
    11461 gaagaatgcg tcccgaacaa tttcatcgcc tttcgcataa agcgatacgc taaatcaacc
    11521 ggcgataatg gcacgggtcc accagaaagt agccgccatc gatccgctga tggtcctgtg
    11581 cagtcttcat gctgataatc aaatttttgc gatttgttag catataaacg actaataaag
    11641 ctttgcattt tttcaaatgc cagcgccctg ttctcttcac ccaacgtgtc gtgataaaag
    11701 cctggcagca gatgcagctc tttcagaggg ctacgtaatc tctgataaaa atcaatctgc
    11761 ggttggcgat gcaccacata gtcatcgcct gatatcagaa gttgcgtggg gagcgtaatc
    11821 gccgccgcat ctctaataat acgttcagac gttttgtaga gatcgagcaa gatattaacg
    11881 gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg ccccccgttg ccgatcgtgg
    11941 gtcaaatagc gtcctttcac ataggaatta ataaaaaaca gaccacgcag acgatgccat
    12001 aacgccagca caggacgtgc cagcggcaca tacaatttaa ccttaaaggc cggagaagcc
    12061 agtaccagcc cgcgaattgc aggtgcataa tcatgaaccc atgtggcaac cagcactgcg
    12121 ccgacgcttt gcgcgatcac taccacctca tccagtccga cctggctgtc actggcagca
    12181 aaacggataa aaagcacgat gggcaatagc aaaaagagga tgggttgtgc gaccaacatc
    12241 aacagcaatc cggtaataag agaaagcgcc agtgcagcaa gattgatgtg attcgctgta
    12301 acatggtgct tataaagcca aaacatcgtc ggtcttaata gcgtctgaaa gagcggtttt
    12361 atctggtaga gcgtcattat caatccttga taattcggtt tgcgctatgg tgcatcaatt
    12421 cccctgacgg agatatcgca acacgccgct tcattaaaaa ttcagaatat atcaaggtat
    12481 tggggactca ctgtcaggaa taattatccc caattaatca aagggttaag aaatattacg
    12541 ttgtggatca atacaactca tcaagttttc tcatcgcttg ttcccgtatt gcgcgcgtct
    12601 taccgttaag cgccagctgg cggactaaat ccagttcagg gttaacaagc tctaacgcca
    12661 gcaaaggatc acgtttcagc ctcgccttta atacatcaac tggaaaacgg ggattattca
    12721 tcgccacaat ccaccactcg ggatcaattt ctgccgccag tactgcaggc ggtgtgtggc
    12781 ggttgttaag tgcgccgaac ttgaaaaatg tgagattgct tttcgccagt gtactccacc
    12841 atatacgatc cggttgtttt gccagtacat ccagttgtgc taacgcatcg gggtcgagtg
    12901 cataatagac gcgtaatgct tcatcaacct gcgcacggga gctgttttcc tgcctcagaa
    12961 aaaacttcgc ccaaatatca ttgcgtggcg tattttgctg taattccttg aggacaacag
    13021 cgggtaaatt atcccaacta actaatgcac tctctaaaaa gtcccagtct tccaaacgta
    13081 tcagggctgt ggcatttaat agcatctgat catcaacttc accgtggctg ataagctcag
    13141 acaaaatcat atcagcgctg gtttcatacg cagctgaatc tgctttcgga ttatttttcc
    13201 gatgcgataa agcacgttgc caaatttttt gtttcatcga cgtgctcaga tactgatttt
    13261 tcgccagatt actccacccc tcaggatcgt tgaaacgtgt caataaccgt tgtagagcag
    13321 cgtcagagtg aattacctgc atatcccgac agcaaaagta ggcaggggat tttttctcca
    13381 tccttttaac aaaattaaac tgtaagttat ctggcaatgc taaaaataac acacgtatta
    13441 gatcatcgtc attctcgaat ttttggtata aataggctgc aactttgtca ctctcttgtg
    13501 gcgtcatccg caataattga ccatggtgtt tcatctctgc cagcttgaac gctaagcttg
    13561 tgacaacatt gtttgatgaa tcgttcgcca actgcattgc ctgattgtag gtgagttgtc
    13621 ctccagcacc gacctgacga atagaggaat catcactgcg gatatagcca tcaataatgg
    13681 attcaggcaa taaccacgca tctttataaa gctcctgaat gatcatatcc caatctcttt
    13741 tcgccagagt cattacgata tccagaggga gattttcacg tagcgcaaga ttcaacggac
    13801 cgacaccatc aggtagacga ctcattcgcg tcattatttc gacagagagc ggttcacggt
    13861 cgagcaaccc cagaacaaca ggctggtaac ccaattgctg atcggtcaac tccagaatct
    13921 cattgtcagg catatatctc gccatacttt caatcgtttg ttcatcattg ctattaataa
    13981 gttgtttgcg taggttagct gaaattcctt taagccttgg gagagttcgg attatggcaa
    14041 gatcaaatgt cgcaatatct cgtccttcaa atgcagcaac gttgccttca tccagcattt
    14101 gcaattcctg cactaactca ggagaaggct tagggcgaaa cagcgcttct tttttcatct
    14161 gattacagac attccctgcc atgcgaatat tgcttcgctt aaaaatttct ttcagagcat
    14221 cttcatcatt tattagaccc caataatatg catcaggatt tattatcatc agcgcttttt
    14281 tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta tttgtcgaag
    14341 acgcaacaac aggtgcaatt atgtcattga taaatatccc acgcgcatta atactcccct
    14401 taatcgtaga cggattatcg taatatgagt cgataaatac cagtccactt gcatttatcg
    14461 aaccggtaat taacagtggt gaatccgtaa ctatattcgt tgccgtcacg ttaccgagaa
    14521 taacccatgg attagaaaga gagaaagtgg aaatatttcc tttgactgtc aaattgccaa
    14581 gaacaatcaa tttttgatta ctctctatat taccttctac cactaaatcg ccacgatgca
    14641 ctttccagtc cgatgcagat aaaccgaagg ggacaagatt cacgctactg ttgatagctt
    14701 gttcaacttg ttgtttatct tcagcggtag ccgcagaaaa aaattgttca atatcaactg
    14761 catttgcaac acatcgaagt tccgcctgta ctggaagact cagcataaac aggggaagta
    14821 aacctatcca gccgcaaatc ctggccttaa acatatgcac tccatgccat taaaaaagaa
    14881 acaaaaggga aatgcatctt tcccttttgt atattcgtta ttctcgcgaa ataccatcca
    14941 acgtagacaa cacttcctca gaaagttgca atttttcagc agccatattc tcccgtaaat
    15001 gcgcaaccga agacgtccct gggatcagca aaatattcgg tgaacgctgt aacagccacg
    15061 ccagcgccac ctgcattggt gttgcaccca ggctcgcagc aacatcggaa agtgtggacg
    15121 attgcagcgg tgtaaagccc ccgagcggga agaacggcac gtaggcaatg ccatcgtggg
    15181 ccaaagcatc aatcattgca tcatcagcac ggtgcgcgat gttgtattcg ttttgcacac
    15241 agacaatttc ggcaatcttg cgcgcctctg caacctgcgt cggtgtgacg ttgctcaggc
    15301 caatatgttt taccaggcct tgttgttgca tctctgccag cacggtcagg ctggcctcaa
    15361 tcgatccttc cgctgggcca tgaccatccc ccatcataac gcgcaggtta accacatcca
    15421 gcacgtccag cccgagatta cgtagattat cgtgcaccgc tttttgcagc tctgccggag
    15481 aaaatgcggg caaccaggat gcgtcctctc cacgccgcgc accaatttta gtgacaattg
    15541 tcaggtcgtc agagtaagga taaagcgctt cgcggataat ctgattggtg acgtgcggac
    15601 cataaaagtc gctggtatca atatgattga cgcccaatgc cagcgcctca cgcagcacgg
    15661 ttatagcgac gtggcgatct cgtggggggc caaaaactcc aggacctgcc agttgcatcg
    15721 cgccataacc aagacggtta acggattttg taccgagagt aaatgtgttg ctgctcatcg
    15781 caccctgccc tgtaataaaa cggattaaga aaaatcagta tagaaacgaa aagagataaa
    15841 taatgcgaag gtgtaaacaa gattactgga tgagttgctc tgttatcctg aaagacacca
    15901 aaacaaaacg cgtcaagcgc cgcatccggc aataaaaaaa ccgcaacctg tatcagcgtt
    15961 gcggtttagg gatcacatgc taaaaattag aacgtccagc atcaggacgt tactgtttcg
    16021 gtcgcagatt ctcatcaaat accaggcgtt tcctgccagg ttcaatttcc cgcaatggct
    16081 cgacttgatg taacgtctgc ttacagcgtg tcaccagcac ctgatactca tgcgtaccct
    16141 gctttttcca tgccagctcc tgctcactaa gttcacgaat cgctttcgcc gggctgccga
    16201 caatcaggta attagcaggc atttctgctt tggctttcac aaatgcggat gcgccaacaa
    16261 tgctgttctc gccaatcacc gcaccgtcca tcactaccgc gttcattccc actaatgcat
    16321 tgcggcggat aatgcagccg tgaaggatag cgctatgacc aatatgtcca tcttcttcaa
    16381 cgacagtatc ctgctcggga aaaccgtgca taacgcaatt atcctgaatg ttcgcgccat
    16441 ctttcaccac gatacgacca aaatcgccac gcagactggc atttggccca acgtaaacgc
    16501 ccttgccgag aataacatcg ccgatcaata ccgctgtcgg atggacaaaa ctctcttctg
    16561 gcacaaccgg agtcagaccg tctatctgat aaattggcat aacgcctcct gttcaatatt
    16621 caagccgcca aaacgttgaa aatacaggct tcccggcgca ggcagttcac cgaccgaggt
    16681 ttcacctttt tcactaacga acgctaaagc agcaggcgct acgcgctgat aaatgttgat
    16741 acacagctga cgtgccgtat gccctgccca gtgtgccgga agcaactcct ccggcaacaa
    16801 tgggtcttta aggacgacac ggcgataaaa atggatcagt aaaagctgaa tatgaaatgc
    16861 ccgctccggg gttaactcgt ctgccgcctc ttttaacagc ggcaccagcg ggcggaatga
    16921 ctgaataaag gtttcgtaca tggcattttg ttcagttaaa tgccagcact cttctactct
    16981 ggcacgcagt gctgcgcgag aaagcgccag tggtatttgc gcttcaaaac aaatcacgtt
    17041 atccgccacc cccgcttcat gcaaaagtgc ctgtacatcg gccagttttt gcgacggcga
    17101 tgccatcagg ctgggtgcca gtgcgccaaa accttgccag atcaactgct ttttgacatc
    17161 agccagcgtc gttttatcta aaccttccga gagcaacagg agccatttac catcccatgc
    17221 aggttgctct gcgcgataaa ttttactttc tgcccgtcgc gtcaggcgca agcctttatc
    17281 actgaggcta tagaaactgc gtcgcccgat gcgggaaaca tccagccagc cttctttatt
    17341 aagacgaaac aaagcggtgc gcacgaaacg ctcaccaaat cccagccctt ccagcaaagc
    17401 agccagacta cccaaccaga tttcaccacc acgatgggaa agcgaatcac catacagaga
    17461 ggagatcaaa gatgtgccac tgaccggaac agcgtttaca gcatgttgga taaaagtatc
    17521 aagtttactc attcgcttca gtctgttgtt attgttgtga tttatgaatc atagcacagg
    17581 tcgtatcgac ttctggccct ggcgctttac cccaccaggg ccagacgata cgtcaggcac
    17641 caacaatatt gcgcagatca aacacccgac acgccttgcc ttctgaacgc gggatactgc
    17701 cacagttaac gatcatcaca tcggtagaga tccccaccat cgacttaatc cgatggcgca
    17761 gctggtggca tacctggcaa cgctgctcat gcgtcaatgt caggctactt tctttcaact
    17821 ccactttcac agaaagtgaa tcaagatgcc cccggcggtt cacctccagt tggtaatgcg
    17881 gcgataaatg ttcgaatttg acaatctctt cttccagttg tgacggaaag acatttacac
    17941 cgcgaataat caacatgtca tcgctgcgtc cgctgatgcg atccatgcgg cgcatagtcc
    18001 gcgccgttcc tggtaacagt cgggttaaat cacgcgtgcg gtaacgaatg accggcaatg
    18061 cttctttggt cagcgtggtg aataacagtt cgccatgttc accatcggca agcggtgtgc
    18121 cgtcatgagg attaacaatt tcaggataga aatgatcttc ccaaatggtt gggccgtcgg
    18181 tagtttccag acactccatc gccacccccg gccccatcac ttctgacagg ccataaatat
    18241 ccagtgcggt gatccccagg cgacgctcaa tctctttacg catggcctgt gtccacggct
    18301 cagcaccaaa tactccaacc cgcagcgagc aaccgctggc atcaccgccc aactgccgct
    18361 ccagctcttc aatcaggtta aggcaataag atggcgtaac catgatcata tctggttgaa
    18421 aatcacggat cagttgcgct tgtttttccg tctgcccgcc agacatcggg atcaccgtag
    18481 cgcccagacg ttcggcacca tagtgcgcac ccagcccacc agtaaacagg ccgtaaccat
    18541 aggcaacatg aattttgtct ttgggcgagc cccctgccgc acgcaaagaa cgcgctacaa
    18601 tattggccca gttatcaata tcgttttgcg tatagccgac aactgtcggt ttacctgtgg
    18661 ttcccgaaga agcatgaatg cgcaccactt gttccattgg cacggcaaag gtgtcaaagg
    18721 gatagttgtc gcgcagatcc tgtttggtgg tacagggaaa tttacgcagg tctgaaagtt
    18781 ccctgaaatc atcaggatgt acgcctgctg cgtcgaattt gcgccgatac atcggcacat
    18841 tttcatacgc gtgtttgagc gtccatttca gacgctgtgt ttgcaacgcc tgtaactcat
    18901 caacagacgc ggtttcaatc gggtcaagct ttgtattggt tatcattgta gggtactcgc
    18961 aggttgatat gctcaaacac gctccagaat catggcgatg ccctgaccga caccgatgca
    19021 catggtgcac aatgcgtaac gaccgttacg ccgatgcagc tcatggctgg cagccagtgc
    19081 caggcgggca ccactcattc ccaacggatg gcctaaggcg atagcgcctc cgttggggtt
    19141 aacatgtggg gcatcatcag gcagccccaa ttcgcgtagt acacccaacg cctgggccgc
    19201 gaacgcttcg ttcagttcaa tcacgtccat atcgtgaata ctcagccctg cgcgttccag
    19261 cacccggcga gttgcaggca ccggaccaag ccccatcagg cgcggttcca ccccggcggt
    19321 tgccatggct acgatacgcg cccgcggtgt cagtccttgc gctgctgcca tctgttcact
    19381 ggcaataatc aacgcagcgg ctccgtcatt caccccggaa gcattgcctg cggtaatcac
    19441 cccattggca cgaaatggtg cttttaaccc acgtaactgt tccagcgtcg tttccgggcg
    19501 cagatgctca tcatgttgta tttctgttac aacacctttc ttgtttttca acacaaccgg
    19561 aacaatctcc tcagccagaa tgcctgagga ttgcgctttt gccgtacgtt gctgactgcg
    19621 tagcgcaaaa ctatcttgat cttctcgtga gatttttaac agttcagcta cattctctgc
    19681 cgtttccggc atgctgtcag ttccaaattg ctgagccatg agcgggttca caaatcgcca
    19741 gccaatagtg gtatcgaaca tctcagcctg acgagaaaat gcactggctg ccttgcccat
    19801 aacaaacggt gcccgtgaca ttgactccac gccaccggcg atcagcaaat cgccatcgcc
    19861 cgctttaatc gcccgtgcgg caaaccccag tgcgtccagc ccggaaccac acaagcggtt
    19921 aatggttgtg ccggaaacac tctgcggcag ccccgccagt aaagtcgcca tccgggctac
    19981 gttacggtta tcttctcccg cctgattagc acagccgagg atcacatcat cgatacactc
    20041 cgcatcgaga cgcgggtttc gcaccagcag ttcccgcaaa gggatagcag ccagatcatc
    20101 agcccgaaca cttgataatg ccccgccgta gcgaccaatt ggcgtacgaa ttccgtcaca
    20161 aataaaggct tcacgcatca ggcttctcct gtaatggtgc cgccgatgcg gtgagattta
    20221 ccgcgaaaca gcgcaaccgt tttttgttgt tggttaacaa tttcgatgtc gtaaacaccg
    20281 gtttgcttgc cctgatgacg tacctgcgca gtagcagtta aggtgtctcc ggcaaagcct
    20341 ggacgcaaaa aatcaatcgt gcaggcagaa gcgacggctg ccagcccctg gctattgcag
    20401 gcgtaggcaa aggcagtatc agccagtgaa aatagctgcc cgccgtggca actttgatga
    20461 ccgttaagca tttgtgcagt gacggtcatg gtcactacag caaagccttc atccattgag
    20521 ataatgtcga tgccaagcgc tttggcgcag gcatcgttct catacattgc atgggcattt
    20581 tgccaggcct tatgactcat aaccgctctc cagaagcgcc cgttggcgca gcaatgaaca
    20641 tgggcgatag cgttcttcgc catagtgatg ttgtagattt tccaggagcc ttaatattcg
    20701 ctgccagcca agttgcgctc cccaggcaag cgggccatat ggataattca ccccaagacg
    20761 catggcggta tcgatatcct gttcagaggc cacgcctttt tgcagcgcat caagggcttc
    20821 attgatgatc attgctaccg ttcgccaaat cagcattcct gggtaatctg caatttgcag
    20881 cactgttttg ccctgctgtt gcaggtaata aatggccttg cgggtcgctg agtccgggtt
    20941 caccgctgcc gcagcaatgg tcaccacctt gcccgccatt ttgtcgacca ctaccaccgg
    21001 gcgtgccagt cgtattgcca gcgcctgtgc cgtctcgcct tgtgtctcaa tcaataaaac
    21061 atcgtcaatt tctgtgacac cgtcactttt cttttctact ttcattgggc taaaactgtc
    21121 gcttaccgct tccaggccaa caactgcctc acgttccgcg cgccagtcgt acacgcccag
    21181 cccacttttc ttgcccaacc gtccaccaat caccagttcc tgttgcacca gcgaaggtaa
    21241 aaaacgacgc tcctgccaga aagcgttaaa caccgaacag gtgacagcaa aattgacgtc
    21301 ctgaccaatc agatcggtta attccagcgg ccccatcggg aaaccagcgc catcgcgaag
    21361 tgcagcgtca atcacttctg gtgcagcaac ctgctcttcc agtgcccgcc aggcctcgga
    21421 ataataagga cgcgcaacac ggttaacgat aaatccgggg gtcgaatgac agcgcactgg
    21481 ctgcttaccc caactcaatg tcagttcaca caactgctcg acaacttccg ctgccgttgc
    21541 cagcccactg accacctcca ccaacttcat caccggtgcc gggttaaaaa aatgcagccc
    21601 cgcaacacgt tcaggatttt ttacctccgc agcaatcgcg gttatagaga ttgacgaagt
    21661 gttagtggtc aatagcgttt gtggcgggca aacttccgcc agttgtgcaa agagcgcttt
    21721 tttaacttcc agacgttcag acgccgcttc aataaccaga ttcgctgctg ccagcgcgtg
    21781 aatatcggtc accgggatca ggcgtttcaa tgtgcgttca caggtttcag cggtcagttt
    21841 tccccgcgtc acgcgtgaat ttagccgcgc gtgtatcccg tcgattgcgc gggtcagcgc
    21901 ttcagcagaa atgtcataca gtaaaacctg atgtccatga ctggcggcaa cttcagcaat
    21961 gcctgccccc atggtgccgc tcccaatcac tgccacagtt tgcacattta tcatcatgct
    22021 atttccccgt gaactgcggt gagcgtttag ccaggaacgc actgacacct tcacgataat
    22081 ctgcgctccg tccggcaagt cgctgatagt cacgttccag atccagttgc gtatcgagcg
    22141 tattggtttc agcgctattt atcgcttgct tgataagtcc aagaccaaat gtcggttgtg
    22201 tcgccagatg ccgtgccagc tgttgcgcgg tatctgccag cgtttcatca tcaacaacct
    22261 gccagatcat cccccattcg tgcgcctgtt cagcactcag ttgattcccc agcagtgcca
    22321 gccccatggc gcgcgctcgt ccggcaacgc gtggcagtaa ccaggttcca ccgcaatcgg
    22381 gtattaagcc taacttacta aacgccatga cgaattttgc tgaacgggca gcaataacga
    22441 tgtcgccccc cagtgccagt gttgcgcctg cccctgccgc cacgccattg actgcacaga
    22501 tcaccggttt tggcagtttt gccaggcgac gtaccagtgg gttatagaaa cgttcaactg
    22561 acatccctaa atccggtgcg gggcctgtgg gatcaacgtt acgatcgtta agatcctgac
    22621 cagcacaaaa tccgcgcccg gcacctgtaa gtaacaggca acggatagtg tcgtcgcgct
    22681 cgacctgttt caggcactct gccagttgtg cgtgcatctc atcattaaaa ctgttcaggc
    22741 gttccgggcg gttgagcgtt agtgtcatca cgcccttttc tacatgactg aggatgaatt
    22801 ccatcattag cgtcctttaa agtcgggcgt gcgtttttgt aagaaagcgg agatgccttc
    22861 atgacgatct tctgttgccg ccagcaaggt gaataactgt cgctcctggg caagtccggc
    22921 ttgcaaagcc acttcctgcg actggcgcag cgcttgcttt gccgcttgta aggccagcgg
    22981 cgagtgacgt gccattttcg atgccagctg taaggcgtat tcgagggtta aatcgctggg
    23041 gaagacgtcg ctaaccagcc cggcctgctg tgcttgctga gcggtgatac tttctccgct
    23101 cagcaccatt ttgctggcta acgatttacc gacactacgg attaaacgtt gcgttccgcc
    23161 tgcgccaggc atgatgccga gagtgatttc cggcaaccca aaacgcgcgt tctctccggc
    23221 aaccaccaca tcgcacaaca atgccagttc gcaacccgcc ccaagcgcgt aaccattgac
    23281 ggctgcgata agtggtttat taaaggcctg caatcgcgcc catagctgcg gacgtgtatc
    23341 gtttaaggtg gccgcgagat ctttttctgc catttcgttg agatcggccc cagcggcaaa
    23401 aaagcgtgca ttaccggtaa tcacacagac cgaaatgctg gtatcggtag ccgcagcttc
    23461 cagttcattt accagttgca tcagcagggc attatttagc gcattacgtg cggcgggacg
    23521 gttaagggtc agcaacaata ctcgttgctg acggctgacg atcagttcgc tcatgccatc
    23581 cccttcgcgt caaagtcaac caccacatcg ctggtcagcg gcagtgcctg gcaactcaac
    23641 acataacctg cggccagttc atccggttcc agactgtaat tggtttccat cgccactttg
    23701 ccacgcagca ctttgcattt gcaggtcgca cagacgccgc ctttgcaggc atagggcaga
    23761 tccgccccct ggcgcaatgc cgcatcgaga atgctttcat cgtcggcatt aagcacgatt
    23821 tcccgatccc gcccatcctg acgtacagtc actttttgtc cgtcactttg cacgttaacg
    23881 ctacgtttga cgcgcgtgcc aggcgtatta aaccgctcca gatgaatggt tttatctggc
    23941 attcccagtg cttttaaggc ggtttccgcg tcatccatca tcgccgccgg accacaaata
    24001 aatgcctcat cataaagacg aaaattaatg agcgaggccc caagtgactg taatttttca
    24061 ccgtcaatac gcccgtgaag cagatcgctg tcgagggttt cctgactgaa aatgcacaac
    24121 aactgtaaac gctgaggata tttgtctttc aggtctgcca gtgcctggcg aaacatcatg
    24181 ctctggctgg tacggttacc gtagatcagg gtgaactgac tttcaggctc ggtttgtaaa
    24241 gtggtggcga taatcgccag cattggcgta atacctgatc ctgctgcaat tgccagatag
    24301 cgcccctggc gttcggcctg cggctgatag ccgaaatgcc cctgcggcac catgacctcc
    24361 agcgtcatac cctggcggat gtgttcgcgg gcatagcggg agaaacgtcc gccttcaatg
    24421 gctttcaccg ccacactaat ttcgccaggc agatagctgc ggcaaatgga gtaacaacgg
    24481 cgtaattctt caccatcaaa gctggctttt aaggtcaaat gttgaccggg gcgaaagcga
    24541 tacgcctcct gcaaaggctg gtgcaccgca aaggtaatgg tcaccgcatc acgggtttcc
    24601 gactcgactt ttgccactgt taaggaatga aacgttgtca tggcatcctc aaatacattt
    24661 gaaataatcg aaaggttcgc ggcaactatc gcagcggtac aatgctttgc aggccgtgga
    24721 accaaattca ctgataagtg tggtatggac gctggcgcag cgcgggcaac gtacttctgg
    24781 cggcaaatgg gcatggcaac tgtgtccggc gggcgggcta atgccatact ctcgcagacg
    24841 ctcacgggca tcgggggtca tccagtcggt ggtccatgcc gggtcgagtt gcagcacaac
    24901 ctgaacgggg gtaaacccgt tggttgtcat tgcctcacgt atcgcgccaa tcaaatgttc
    24961 cgttgccgga caaccggaat atgtcggcgt aaagccgatc acccatcctt ctcccatctg
    25021 tgtcacatta cgcaccatgc ctaaatcagt aatggtcagc accgggatct ccgggtccgg
    25081 gatctggctg agtagcgccc atatctcatg aacctgtggc ggtgcaatgg tggccagacg
    25141 ttgcataccc atctcctctg ttaccattgc tgaccgggca agacacgctg gagatactgc
    25201 atttctgcca gcatgggtcc aaggtgttcg gtatgcagtc cttttttacc gccagtgcga
    25261 tacgcctgct cttgcggtac gttcaatgtg gcttcgttga tcccggcaaa aacttcggct
    25321 tcccacgctg cgcgtaaagt gcgtggatca accgcaatac cctcttcact cagtgcaata
    25381 tcaatctcgt cggcatcgaa cagttcggcg gtaaaacgcc acaacttgtt gatggcctgc
    25441 tgcatctttt gccctgatac gtcagtacca ttgcccagtc gctccagcca gccacgacta
    25501 aaacgcaggt gatagcgcgc ttctttaatt gctttggcag aaatcgccgc cagttgcgga
    25561 tcacggcttt ccatcagacg ggtaaagagc gccacatgcc atgcatcgat gaaatactgg
    25621 cgtgcaatgg tgtcggcaaa attgccgttt ggttgttcaa ccaacaataa gttgctgaac
    25681 tggcgctcgt ctcgggtgaa ggccagggta tcttcatcgc cttctcccgc taattcagcg
    25741 gcatacgata agaagttgcg tgcctgacct aataaatcga ggccaatgtt tgccagtgcg
    25801 agatcgattt ccagttccgg tgcgtgaccg caccattcac ccaaccgctg ggagagcacc
    25861 aggcagttat cgcccaggcg caaggtgtaa gccgttaact gattcatttc cgacctcaca
    25921 tgtgctcaat gccatcaggg atggtgtaaa acgttggatg gcgatagacc ttgctttcag
    25981 ccggatcaaa aaattcaccg cgttcttccg gttgcgaggc aacaatttca ctcgccttca
    26041 ccacccaaat tgaacatcct tcgctacgac gggtgtaagc atcacgggca ttttccagtg
    26101 ccatccgctc atcggcagca tgtaaactgc caacatggcg gtgtgataag ccctgtttgc
    26161 cacgcacgaa aacttcgtat aacggccagt aaacattact cattttgaat ctccttatgc
    26221 gaccttacgg gcatgttgtt tttgtgcatg ggccagcgct gcttcccgta cccaggtacc
    26281 ttcttcccag gctttacgtt tggcgtcgag cctctcctga ttacaaattc cgcgaccgtt
    26341 aattacttcg ttaaactcct gccagtcgat ctcaccaaag cggtagtgac cgctttcagt
    26401 gtcaaaatgc agatccgggt caggaacggt cataccgagc atttcaacct gtggaacggt
    26461 gttatccacg aaacgctggc ggagttcgtc gttggtgaaa cgtttgattt tccaggtgag
    26521 acttctggcg ctgtttggcg agttatcatc gtttggcccg aacatcatta aggctggcca
    26581 ccagaaacgg ttaatggcgt cttgcaacat ctgcttttga gcttcgctgc cctgcgccag
    26641 tgccatgcag gcttcaaacc cctgacgctg gtgaaaactc tcttctttgc agattttcac
    26701 cattgctctg gcatacgggc cataagaagt gcggcataac gccacctgat tgacgatcgc
    26761 tgcgccatcc accagccagc cgataacacc gatatcagcc caactcaatg tcggataatt
    26821 aaagatggag gaatatttca tccgtccgtc gagcattttt tggtagatgt cttctctggc
    26881 gcagcccagt gtttcggcgg cgctatagag atacagacca tgaccggctt catcctgcac
    26941 tttggccaac agaatggctt tacgccgcaa ggttggtgcg cgagtgatcc agttaccttc
    27001 aggcaacata ccaacaatct cggagtgcgc atgctgccca atctggcgga tcaatgtctt
    27061 tcggtaagca tcgggcatcc agtcctgtgg ctcgatagcc gtttcctggg ctatccgttg
    27121 ctcaaagcgt tcttcttggg tcacgtcatc acctttacga ttccagtttt cgtgaattaa
    27181 ttaaaaacac gaatcacatt gttttatgaa agttacacaa tagttaagta aaatcacaaa
    27241 ttttgtatgt ttgaactgtg acggatttcg caccctattt ttataaatca ttatttatca
    27301 gtgcattaat gttttccgca gttgctatac agatcgcata gttaacattt cgttaaaaga
    27361 tcccttgctt tttatgattc gcgatttaac tattagcaac agaaatgtga aacatctgga
    27421 gagtagcgat gcagcagtta gccagtttct tatccggtac ctggcagtct ggccggggcc
    27481 gtagccgttt gattcaccac gctattagcg gcgaggcgtt atgggaagtg accagtgaag
    27541 gtcttgatat ggcggctgcc cgccagtttg ccattgaaaa aggtgccccc gcccttcgcg
    27601 ctatgacctt tatcgaacgt gcggcgatgc ttaaagcggt cgctaaacat ctgctgagtg
    27661 aaaaagagcg tttctatgct ctttctgcgc aaacaggcgc aacgcgggca gacagttggg
    27721 ttgatattga aggtggcatt gggacgttat ttacttacgc cagcctcggt agccgggagc
    27781 tgcctgacga tacgctgtgg ccggaagatg aattgatccc cttatcgaaa gaaggtggat
    27841 ttgccgcgcg ccatttactg acctcaaagt caggcgtggc agtgcatatt aacgccttta
    27901 acttcccctg ctggggaatg ctggaaaagc tggcaccaac gtggctgggc ggaatgccag
    27961 ccatcatcaa accagctacc gcgacggccc aactgactca ggcgatggtg aaatcaattg
    28021 tcgatagtgg tcttgttccc gaaggcgcaa ttagtctgat ctgcggtagt gctggcgact
    28081 tgttggatca tctggacagc caggatgtgg tgactttcac ggggtcagcg gcgaccggac
    28141 agatgctgcg agttcagcca aatatcgtcg ccaaatctat ccccttcact atggaagctg
    28201 attccctgaa ctgctgcgta ctgggcgaag atgtcacccc ggatcaaccg gagtttgcgc
    28261 tgtttattcg tgaagttgtg cgtgagatga ccacaaaagc cgggcaaaaa tgtacggcaa
    28321 tccggcggat tattgtgccg caggcattgg ttaatgctgt cagtgatgct ctggttgcgc
    28381 gattacagaa agtcgtggtc ggtgatcctg ctcaggaagg cgtgaaaatg ggcgcactgg
    28441 taaatgctga gcagcgtgcc gatgtgcagg aaaaagtgaa catattgctg gctgcaggat
    28501 gcgagattcg cctcggtggt caggcggatt tatctgctgc gggtgccttc ttcccgccaa
    28561 ccttattgta ctgtccgcag ccggatgaaa caccggcggt acatgcaaca gaagcctttg
    28621 gccctgtcgc aacgctgatg ccagcacaaa accagcgaca tgctctgcaa ctggcttgtg
    28681 caggcggcgg tagccttgcg ggaacgctgg tgacggctga tccgcaaatt gcgcgtcagt
    28741 ttattgccga cgcggcacgt acgcatgggc gaattcagat cctcaatgaa gagtcggcaa
    28801 aagaatccac cgggcatggc tccccactgc cacaactggt acatggtggg cctggtcgcg
    28861 caggaggcgg tgaagaatta ggcggtttac gagcggtgaa acattacatg cagcgaaccg
    28921 ctgttcaggg tagtccgacg atgcttgccg ctatcagtaa acagtgggtg cgcggtgcga
    28981 aagtcgaaga agatcgtatt catccgttcc gcaaatattt tgaggagcta caaccaggcg
    29041 acagcctgtt gactccccgc cgcacaatga cagaggccga tattgttaac tttgcttgcc
    29101 tcagcggcga tcatttctat gcgcatatgg ataagattgc tgctgccgaa tctattttcg
    29161 gtgagcgtgt ggtgcatggg tattttgtgc tttctgcggc tgcgggtctg tttgtcgatg
    29221 ccggtgtcgg tccggtcatt gctaattatg ggctggaaaa cttgcgcttt atcgaacccg
    29281 taaagccagg cgataccatc caggtgcgtc tcacctgtaa gcgcaagacg ctgaaaaaac
    29341 agcgtagcgc agaagaaaaa ccaacaggtg tggtggaatg ggcggtagag gtattcaatc
    29401 agcatcaaac cccggtggcg ctgtattcaa ttctgacgct ggtggccagg cagcacggtg
    29461 attttgtcga ttaatcggtg aatgaagggc aacggcgaat agttgccctt ttatttcact
    29521 aaattttgtg acgttgtcac attatgcata atgtgtacat ctattttcag ggcatccact
    29581 gtatgaaaag ctggcacacc tgccaaacaa cctggcaggt gcaggcaatc ccctttgcat
    29641 cagtactgat aatgtgaacc tgactaaacc gcccacagag cgcggttgct aacaagaaca
    29701 caacatctga cgaggttaat aatgggaagc ccctctctgt attctgcccg taaaacaacc
    29761 ctggcgttgg cagtcgcctt aagtttcgcc tggcaagcgc cggtatttgc ccacggtggt
    29821 gaagcgcata tggtgccaat ggataaaacg cttaaagaat ttggtgccga tgtgcagtgg
    29881 gacgactacg cccagctctt taccctgatt aaagatggcg cgtacgtgaa agtgaagcct
    29941 ggtgcgcaaa cagcaattgt taatggtcag cctctggcac tgcaagtacc ggtagtgatg
    30001 aaagacaata aagcctgggt ttctgacacc tttattaacg atgttttcca gtccgggctg
    30061 gatcaaacct ttcaggtaga aaagcgccct cacccactta atgcgctaac tgcggacgaa
    30121 attaaacagg ccgttgaaat tgttaaagct tccgcggact tcaaacccaa tacccgtttt
    30181 actgagatct ccctgctacc gccagataaa gaagctgtct gggcgtttgc gctggaaaac
    30241 aaaccggttg accagccgcg caaagccgac gtcattatgc tcgacggcaa acatatcatc
    30301 gaagcggtgg tggatctgca aaacaacaaa ctgctctcct ggcaacccat taaagacgcc
    30361 cacggtatgg tgttgctgga tgatttcgcc agtgtgcaga acattattaa caacagtgaa
    30421 gaatttgccg ctgccgtgaa gaaacgcggt attactgatg cgaaaaaagt gattaccacg
    30481 ccgctgaccg taggttattt cgatggtaaa gatggcctga aacaagatgc ccggttgctc
    30541 aaagtcatca gctatcttga tgtcggtgat ggcaactact gggcacatcc catcgaaaac
    30601 ctggtggcgg tcgttgattt agaacagaaa aaaatcgtta agattgaaga aggtccggta
    30661 gttccggtgc caatgaccgc acgcccattt gatggccgtg accgcgttgc tccggcagtt
    30721 aagcctatgc aaatcattga gcctgaaggt aaaaattaca ccattactgg cgatatgatt
    30781 cactggcgga actgggattt tcacctcagc atgaactctc gcgtcgggcc gatgatctcc
    30841 accgtgactt ataacgacaa tggcaccaaa cgcaaagtca tgtacgaagg ttctctcggc
    30901 ggcatgattg tgccttacgg tgatcctgat attggctggt actttaaagc gtatctggac
    30961 tctggtgact acggtatggg cacgctaacc tcaccaattg ctcgtggtaa agatgccccg
    31021 tctaacgcag tgctccttaa tgaaaccatc gccgactaca ctggcgtgcc gatggagatc
    31081 cctcgcgcta tcgcggtatt tgaacgttat gccgggccgg agtataagca tcaggaaatg
    31141 ggccagccca acgtcagtac cgaacgccgg gagttagtgg tgcgctggat cagtacagtg
    31201 ggtaactatg actacatttt tgactggttc ttccatgaaa acggcactat tggcatcgat
    31261 gccggtgcta cgggcatcga agcggtgaaa ggtgttaaag cgaaaaccat gcacgatgag
    31321 acggcgaaag atgacacgcg ctacggcacg cttatcgatc acaatatcgt gggtactaca
    31381 caccaacata tttataattt ccgcctcgat ctggatgtag atggcgagaa taacagccta
    31441 gtggcgatgg acccagtggt aaaaccgaat actgccggtg gcccacgcac cagtaccatg
    31501 caagttaatc agtacaacat cggcaatgaa caggatgccg cacagaaatt tgatccgggc
    31561 acgattcgtc tgttgagtaa cccgaacaaa gagaaccgca tgggcaatcc ggtttcctat
    31621 caaattattc cttatgcagg tggtactcac ccggtagcaa aaggtgccca gttcgcgccg
    31681 gacgagtgga tctatcatcg tttaagcttt atggacaagc agctctgggt aacgcgttat
    31741 catcctggcg agcgtttccc ggaaggcaaa tatccgaacc gttctactca tgacaccggt
    31801 cttggacaat acagtaagga taacgagtcg ctggacaaca ccgacgccgt tgtctggatg
    31861 accaccggca ccacacatgt ggcccgcgcc gaagagtggc cgattatgcc gaccgaatgg
    31921 gtacatactc tgctgaaacc atggaacttc tttgacgaaa cgccaacgct aggggcgctg
    31981 aagaaagata agtgattgtt tcagacaaaa aaacggcacc aggtgccgtt ttttacttat
    32041 gagcgaacca gattaatacc gtacacacac cgacttagtt tcacaccaac cgtccagcca
    32101 gtcggggcca aaatcacggc ccgttcctga ctgcttcatc ccaccaaacg gtaagttagc
    32161 gtcaattaag gtatggctgt ttacccacac cgtccctgcc tgtaagcgat cgctatattc
    32221 cagagcctgg gagagatttt gcgtccagac actggcagtt aagccatatt ccgtgtcgtt
    32281 tgccagttgt aacgcctctt ctccatccgc tactcgcacc aggtttacca ccggaccaaa
    32341 cacctcttca cgagttaagc gcaatttagc atcgggattt accaccagcg ttggcgcaac
    32401 ataatacccc tctccggctg gtccattcga cccgcgaatc agctctgctt gctgtgcctg
    32461 cgcatcgtcg aggaatgaac acactttgtc gcagtgcgca cgagaaacca aagggttaat
    32521 ctgtgcaaca ggtgacatcc ccggtcccac ttgcaacgat tttaccgcct gctcaaatcc
    32581 actaaccagc gtgtcaaaca acggcgcttc aatataaatt cgcgaactgg cggcgcatac
    32641 ttgcccttga ttcaggaagc ttccggtcat caagccttca ataacccatt gcggatcagc
    32701 atcttttaat acaattgccg ggtttttacc gcccagttcc agcgttacac gcgttaagtg
    32761 atcagcagca gttctggcaa taccttttcc cgtcgcggtt gaaccggtaa aactgatttt
    32821 cgcaacatga ggatgtgacg tcagggccgc gccgcataca gcacctgacc cggtgacgac
    32881 attaaaaacg ccatcaggga taccggcctc gctggccagt tccgccacgc gcaacatcgt
    32941 cagtggcgtg gtttccgaag gcttaatcac gattgaacag cctgctgcca gtgctggcat
    33001 caccttccac ataccaatca tcaacggaaa gttccatggc acaattcccg ccactacgcc
    33061 aaccggctct ttacgcgtcc aggcctgata acgcgccccc tggggtaagg gaatcgacaa
    33121 gtccagcgtt ttacccgcga ttttggtcgt taacccggcg gtataacgca tccagttcag
    33181 cgtacagccc acttcaaaag cacgggaaat ggcaattgac ttgccttgct ccagggtttc
    33241 cagttgcgcc agctcctcac tgtgctgctc caccagatca gcaaaacgta gcagaatacg
    33301 ttcacgctct gcgggtaatc gcccggccca gcgacgcgag acaaaggccc gccaggcaga
    33361 catgactgcg ttatctacat ccgcttcgtt ggcatcagca gtagacgcaa tttcttgccc
    33421 ggtggccgga tcaaagatcg ccaaccgttt ttcactttgt gcggggccag gacgaccatc
    33481 aatataaaga ccgtgttgac gatcgagaaa ctgttggacc tggcttaata ctgctacatg
    33541 cggctctgtc atcacttttc cttattattt acccagtgtg atttcagtgt acgcagcgac
    33601 aagaaaagtc acttttttcg ctgtgacatt cgctttgtca gctgtgacaa gctccgcaaa
    33661 tcgtgacaat aaccgcttat gtctctatgc ctgacaagac ttatttccta tagtcaggct
    33721 cattagcttc gtttattgca acacaaatgc aacaataaaa atacatttca cagagcgaaa
    33781 acgtgccatg aaccccgcag tggataatga gtttcagcaa tggctttccc aaatcaatca
    33841 ggtatgcgga aattttaccg gacgcctgct gactgagcgt tacacgggtg tactggacac
    33901 gcattttgcc aaaggactaa agctgagtac cgtgacaacc agcggggtga atttatcccg
    33961 cacctggcag gaagtaaaag gcagcgacga tgcctggttt tacaccgttt ttcagcttag
    34021 tggtcaggca ataatggagc aggatgagcg tcaggtgcag attggcgctg gcgatattac
    34081 gttactcgat gcctcacgcc cctgttcgct ttactggcag gagtcttcta aacagatttc
    34141 attacttttg ccacgcactc tgctggaaca atattttccc catcaaaaac ctgtctgcgc
    34201 agaaagactg gacgctgact tacccatggt gcaactcagt catcgcctgt tacaggagag
    34261 catgaataat ccggcacttt ctgaaacaga aagtgaagct gcgctacagg cgatggtgtg
    34321 tctgctgcgc ccggtacttc atcagcggga atctgttcaa cctcgtcgtg aacgtcagtt
    34381 tcaaaaagtg gttacgttga tagacgataa tattcgcgaa gagatattac gcccggagtg
    34441 gatagccgga gagacaggta tgtcagtacg tagtttgtac cgaatgtttg ccgataaagg
    34501 tttggtagtc gcgcaatata ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg
    34561 ccatgccgca gatgatgaaa aactggcagg catcggcttt cattggggat tttctgacca
    34621 gagtcatttt tcaacggtat ttaagcaacg ctttgggatg acgccaggcg agtatcgacg
    34681 taaattccgc taactaattg gcgttgcatg tactgcaacg ccaattagtt aaaacagata
    34741 tttgactatg catttttcag ggcaaaaggc aatttgacag gagtttttga atattcacct
    34801 tgtttcagga aaatagatca atcgccttct cctgtagcgt tatcccgata ttgcggtttt
    34861 gctattaaaa ttttcgcacc catttaccgt ttatcccttg tacatattcc cccggtttgg
    34921 cccgagctac gagcttttgc ccggcgagtt tcgcgatatc atctaccgac acattatttt
    34981 gctttgccag ttgttggtaa ctcgctttac gttcggcatt aatgtcggct actaatttct
    35041 ctgtttcagc atccgttttc agcgcaacca gataaccgta aaatgtttca cctacccgcc
    35101 cctgagttct ggcttcatcc agagtcaatg ccattacatt gctgcttacc agcccaacaa
    35161 gaaacgcaca aagaagtaat gttttcttca tcatcacctc agaaaagatc gctacgagtt
    35221 tcaagcagct cttcgacatc tttgtctgcc ttgatgatga tctcatgctc aattttaacg
    35281 ttcatattga tagtgatcgg ttccttaggt gcagcgactt caatgcgagg cgtacagcca
    35341 accagcataa aagatgacgt caacgcagcc agtaaaattt tcattgtttt tcctcacact
    35401 cttttccttg cggacagtca tttcccggca gacgtgcgtt ctgctccagc catgcctgga
    35461 gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat attctcttca tgatggtaat
    35521 taaggtttac cgtaccgctt ttaccatcaa cgcgactggt acctgtaatg ttggcctgca
    35581 tggttaataa accgagatta tctaaattaa tttttgtcga tgaacggcta atttccatat
    35641 agcgcaacca gttaattgct gaacccgcag tcatattgtc ttttaccacc gcatccgcag
    35701 tgtctttatc cagtcgcaat gtcatcggcc cgctattcgc cagccagcca tctttcacaa
    35761 tccatttttc attgttcagc cataacggca gtgcaccact aaatgccccg gacatggcga
    35821 attgtttcgg attgacggcg ctaaccagtt cgctggatga gagattattc agccgcaaca
    35881 gcgccgggtt atgttgcggc atacgtaatt gtttcatcag tacgttaccg cctaacacat
    35941 cgacgctaac atcagtcaac agcaagggtt cttcttcagt ccacggataa cgcccttgca
    36001 aatcagccgt aatatttttc gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa
    36061 cggggccgcg agtccccaga tgccaggctc catcggcaaa acggaaaggc aggacaaaat
    36121 cgacaccgtt aacctgatta tctggcatcc aggcactacc gcctttcaac acgccgtgtc
    36181 ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc aacctgagca tacagttcac
    36241 catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg ctggaatacg gtcagtgatt
    36301 gtttaggcca ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca tttacccgaa
    36361 ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag gaaataggta ggatcgagcc
    36421 catcgacgct aaattttaag gttgatggcg gtaacacact gccgccagtg aacagcgttt
    36481 gcccggcgtc cagtgacagc gcgccgctaa aggagggatg ctgtgcgtca cgtacccaga
    36541 cgatgggctt gtcgagaatt aatcgcggct tttctaccgt catcgtaccg tattgtaact
    36601 ggtcgaaacc ggtggagaga tcggtcagcg taatcgtgct gtcatgccac tcaccttttc
    36661 ctgcgacatc ccaggtggca ttcatcggtg taaaactccc ttttccccag tagcgccact
    36721 gccagcggcc agcgtcaggg agaaaatcat tcgccagccc atccatatgc agcacgaaat
    36781 cgcccagttc attttcatgc gcctgcaaga tggcctgcaa acgtccgtca acaccacgtt
    36841 gggtgacttt tacacccgct aaaggccagc ggatttcatc gatgtccagc gaatcgatga
    36901 ctcttccctt tgaacgaagt aacgcgcctg gctcaaaggt cagcgttggg tcagacagac
    36961 ttccacttag ctgcgcaggt aaacgggcat ataaaatgag gtccgcctgt ttcgcttcac
    37021 cggtcagctg cagaggcagc tgactgttat ccatgcttaa ttttcctggg ccaaaattaa
    37081 gcaccgcgtt gcccttaccc gcttgcccct gggtcagcac actcagtcgt ccgctgacca
    37141 gagcgttctc aagccctgcc tgccagttgt cgactttgac acccagtcgg ccactcaaag
    37201 gaaaacctgc atacggccag ctccagcgac catcgcttac ggtcaattgt tgacgagtaa
    37261 tttgccacgg caaatcgagc aacggatcgc cgttatcccg tgccagcacg atcaattgcc
    37321 cgctattttc ctgccagtcc agctcggcat ccaccagtga cggttcctgc ggcaagttta
    37381 acgtcgcagt agcatgccca cttacaggaa gtccatccgg cacgagcggc atagcaaatt
    37441 cccccaccag ttttaccggc ggctgatttt caaacgcgac gacatccagt tcgctgactg
    37501 taagttgttg ccctttcagc tggccttgaa atttaacttt ttcgccctga taacgcagtt
    37561 gctggatatc agaggttaat gcgagagaga gttttccctg ccattcctgc cagggagaaa
    37621 aaatcagttt atcgatattg atccaggtgt taggcagcat ggcctgccac tgcgcgagtg
    37681 tttttggagc ggctggcgat tgctccgtct gcggcaattt cgccagacaa gcagaatcaa
    37741 gttctaccgt gccgacgttc aataaccagc ggctgggatg tgaaaggctg gcgttggtga
    37801 tatgcgcaag ctgacaatct cccaccagat aacggagatc gggaatgatt aaacctttac
    37861 gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg
    37921 ccagcgtggg aacccacagc ccgagcgtca tcagcagcgt caacggcaca agaataatca
    37981 gtaataacag cgcgagaacg gctttatatt tacccagcat gggtagttaa tatcctgatt
    38041 tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg tttaaccgtt cagttgaagg
    38101 ttgcgcctac actaagcata gttgttgatg aatttttcaa tatcgccata gctttcaatt
    38161 aaatttgaaa ttttgtaaaa tatttttagt agcttaaatg tgattcaaca tcactggaga
    38221 aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt acctgcaaca
    38281 ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga cggaaaaaac
    38341 cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg acggcagccg
    38401 cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg
    38461 tttcaataac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc
    38521 agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga cgctgaaccg
    38581 ccgtattcac cgcgcgtatc agcgtacccg tgatgctaac ttctctctgg aaggtctgac
    38641 cggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa tcggtgtggc
    38701 gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc cgtatccaag
    38761 tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc
    38821 agacgttatc tctctgcact gcccgctgac accggaaaac tatcatctgt tgaacgaagc
    38881 cgccttcgaa cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg gtgcattgat
    38941 tgattctcag gcagcaattg aagcgctgaa aaatcagaaa attggttcgt tgggtatgga
    39001 cgtgtatgag aacgaacgcg atctattctt tgaagataaa tccaacgacg tgatccagga
    39061 tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc accaggcatt
    39121 cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact taagcaatct
    39181 ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct tgccgctccc ctgcattcca
    39241 ggggagctga ttcagataat ccccaatgac ctttcatcct ctattcttaa aatagtcctg
    39301 agtcagaaac tgtaattgag aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc
    39361 ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg ttacgccaga acagctacag
    39421 catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg
    39481 ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa ccgctttagc
    39541 ggtgaaggca aactgtctaa tggtgaactg acagccaaag ggctggcaat gacccgtatg
    39601 atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat gctgaaagaa
    39661 ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcaaa acagacatta
    39721 acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc ggcaagtgac
    39781 tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt accatccagt
    39841 tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc actactggac
    39901 atcgacgctt ttaaacctgg cgctacgtgc gcggcagtcg cctgctgaac aggttcacta
    39961 ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg catcttttct
    40021 tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca gagggcggcg
    40081 tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat ttttttgccc
    40141 tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga aaatgcagaa
    40201 gaaagccatt tgctaaaatt gaaagattac tactgggcgc gcagcaattt cgtgcgcccc
    40261 tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc gcggttgctt
    40321 cggtcgcatt tcgcaccagt gaagttatcg ccatctaccc tattaccccc agttccacga
    40381 tggcagaaca ggctgatgcc tgggccggaa acggcttaaa gaacgtttgg ggagacacac
    40441 cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc
    40501 agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg atgatcccga
    40561 cgctgtacaa actggcaggc gaactaacac cgtttgtcct gcatgtagcg gcacgtaccg
    40621 ttgccacaca tgcactctct atttttggcg atcattccga cgttatggcg gtgcgccaga
    40681 cgggttgcgc gatgttgtgt gcagcaaacg tccaggaagc gcaagacttt gctctcattt
    40741 cgcaaatcgc gacgctgaaa agccgcgtgc catttattca tttctttgat ggtttccgca
    40801 cgtcccacga aatcaataaa attgtcccgc tggccgatga cacgattctt gatctcatgc
    40861 cgcaggtcga aattgatgct catcgcgccc gggcactcaa cccggaacat ccggtgatcc
    40921 gcggtacgtc cgccaatcct gacacttatt tccagtctcg cgaagccacc aacccatggt
    40981 acaacgcggt ctatgaccat gttgaacagg cgatgaatga tttctctgcc gcgacaggtc
    41041 gtcagtatca gccgtttgaa tattacgggc atccgcaagc ggaacgggtg attatcctga
    41101 tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc cgtggcgaaa
    41161 aagttggcgt gctgaaagtt cgcctgtacc gccccttctc cgctaaacat ttactgcaag
    41221 ctctgccggg atccgtacgc agcgtggcgg tactggacag aaccaaagaa cccggtgccc
    41281 aggcagaacc gctctatctg gatgtaatga ccgcactggc agaagccttt aataatggcg
    41341 agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg tctttcatcc aaagaatttg
    41401 gcccagactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct
    41461 ttacggttgg tatttacgat gatgtgacca atctgtcact gccgttgccg gaaaacaccc
    41521 tgccaaactc ggcgaaactg gaagccttgt tttatggcct tggtagtgat ggcagcgttt
    41581 ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac gcacagggct
    41641 attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt cgagtgagcg
    41701 aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc tgccaccagt
    41761 tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc attttcctgc
    41821 tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg
    41881 tgttaaacca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc gcccgcgaat
    41941 gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat ctgacgcaaa
    42001 ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa agttacagta
    42061 gcaaaggcca ggatctggtg gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg
    42121 tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt
    42181 ccgatgccgc ccctgatttc gtgaaaaccg taaccgctgc gatgctcgcc gggcttggtg
    42241 acgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc actacgcgct
    42301 gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc tgtacccaat
    42361 gtaaccactg cgttgccgct tgcccacact cagctattcg cgcaaaagtg gtgccgcctg
    42421 aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc
    42481 gcgggcagaa atatgtcttg caggtggcac cggaagattg caccggttgt aacctgtgcg
    42541 tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc aatatgatgt
    42601 ctcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc aacctgccag
    42661 aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg attacaccgc
    42721 tgtttgaata ttcaggtgct tgctccggtt gtggcgagac gccgtatatt aaattactga
    42781 ctcagctcta tggcgaccgg atgttgatcg ctaacgccac tggctgttct tcaatttatg
    42841 gcggtaacct gccctctaca ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg
    42901 cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg acggtcgatc
    42961 aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc ccggcggaat
    43021 tactgacggc gttgaaatca gacgccacgc cagaggttcg tcgtgaacag gttgcagctt
    43081 tacgccagca actcaacgat gttgccgaag cacatgaact gctacgtgat gcagatgcac
    43141 tggtggaaaa atcaatctgg ctgattggtg gtgatggctg ggcttacgat atcggctttg
    43201 gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg ctggatacgc
    43261 aatgctattc caacaccggt ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta
    43321 aatttggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt
    43381 acggtcatgt ttatgtggcg cagatttctc tcggcgcgca gctgaaccag acggtgaaag
    43441 cgattcagga agcggaagcg tatccggggc catcgctgat cattgcttat agcccgtgtg
    43501 aagagcatgg ttacgatctg gcactcagcc acgaccagat gcgccaactc acagctaccg
    43561 gcttctggcc gctatatcgc tttgatccgc gtcgtgccga tgaaggcaaa ctgccgctgg
    43621 ccttggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac gagcaacgtt
    43681 tccgtcggct gaattcgcag cagccagaag tggcagaaca gttatggaaa gatgctgcag
    43741 ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca
    43801 acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaaagc ccggacgact
    43861 gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc ccaacagaaa
    43921 ataaaatctg tgacaataaa ggcatataac ccgcgcagaa taacgtatag caaattaaaa
    43981 gtataatttt tattttgtat gtattatctt cgatacattc accagactta ttatcatttc
    44041 agaggaatta tttgattaag gttttactta aggcgtaaca aatgatattt atcggctaac
    44101 tgaacttctc ctttaggatg ttttcactcc ccgaaatggg gataaaaggc aaataaaata
    44161 actaaaggat ttattcaatg aaaagcaaag tactggcact tttaattcct gccctgctcg
    44221 ccgcaggtgc tgcacatgca gccgaagttt ataataaaga cggcaacaaa ttagatctgt
    44281 atggcaaagt tgatggcctg cattattttt ctgataattc agcgaaagat ggcgaccaga
    44341 gctatgcgcg tctgggtttt aaaggcgaaa cccaaattaa cgatcaactc actggctacg
    44401 gtcaatggga atacaatatt caggcaaaca acactgaatc ttcaaaaaac cagtcatgga
    44461 cccgtctggc atttgccggg ctgaaatttg cagattacgg ttctttcgat tacggacgta
    44521 attatggcgt aatgtacgac atcgaaggct ggaccgatat gctgcctgaa tttggcggtg
    44581 actcttatac caatgcagac aactttatga ctggtcgagc caatggcgtc gcgacttatc
    44641 gtaatactga tttcttcggt ctggtaaatg gtctgaactt cgcggtgcag tatcaaggta
    44701 acaacgaagg agccagtaat ggtcaggaag gcaccaacaa cggacgtgat gttcgccatg
    44761 aaaacggtga cggctggggt ctttccacaa catatgattt aggcatgggc tttagcgctg
    44821 gtgcggcata cacctcttct gaccgcacca atgaccaggt taaccatact gcggcgggtg
    44881 gtgataaagc agacgcgtgg actgctgggc taaaatacga tgctaacaat atttacctgg
    44941 caaccatgta ttcagaaacg cgtaatatga ccccgtttgg cgacagcgat tatgctgtgg
    45001 caaacaaaac ccagaatttt gaagtcactg cacagtacca gtttgatttt ggtctgcgtc
    45061 cggcagtctc tttcctgatg tctaaaggcc gtgacctgca cgctgcgggt ggtgcagaca
    45121 acccggcagg tgttgatgat aaagatctgg ttaaatacgc cgatgttggc gcgacttact
    45181 atttcaataa aaacatgtcc acctacgttg actataaaat caacctgttg gatgaagatg
    45241 acagcttcta cgctgccaat ggcatctcta ccgatgatat tgtcgcttta ggtctggttt
    45301 atcagttcta aatcctcctg cccgctgtta tggcgggctt tttctgctta ttcttcctcc
    45361 tttgatctaa attaaaaatg tgaactccgt cattacacaa aaagtgtcat ctggcgttac
    45421 actttatgcg gatactaaaa caggaggttt tatgaacaga acgattcttg tccctatcga
    45481 tatttccgat tcagaattaa ctcaacgcgt gattagccac gttgaggaag aggcaaagat
    45541 tgatgatgca gaggttcatt tcctgacggt aataccttca ctgccctact atgcctctct
    45601 gggtttagcg tattccgcag aattaccggc aatggatgac ctgaaagcgg aagccaaatc
    45661 gcaactggaa gagatcatta aaaaatttaa actgccaacc gacagagtgc atgtccatgt
    45721 tgaggaaggc tcgcccaaag accgcattct ggaattggcg aagaagatcc ccgctcatat
    45781 gatcatcatt gcttcccatc gaccggatat caccacttat ctgctcggtt ccaacgccgc
    45841 agctgtagtg cgtcacgcag agtgctccgt gctggttgtg cgctgacact aacgcccgca
    45901 cattgctgcg ggctttttga ttcatttcgc aaatgtgctg acattttccc ctctaatccg
    45961 taccatacac gccacagttt tttatatcag atttcttatg ctgggcgttc cggcatgacg
    46021 catactcttc tgatgccata taacgaattg agtcgctttt aaatgtcgca aaatcaagaa
    46081 attagtaaga aagaacaata caacctgaac aaattacaaa aacgtctgcg tcgtaacgtg
    46141 ggcgaagcca ttgctgactt caatatgatt gaagaaggcg atcgcatcat ggtttgcctc
    46201 tccgggggta aagacagcta taccatgctg gagattctgc gcaatttgca gcaaagcgcg
    46261 ccaatcaatt tttcgctggt ggctgttaac ctcgatcaaa agcaaccggg cttcccggaa
    46321 cacgttctgc ccgagtatct tgaaaagctg ggcgttgagt acaagattgt tgaagagaat
    46381 acttacggta tcgtgaaaga gaagattcca gagggcaaaa ccacttgctc actgtgttct
    46441 cgccttcgtc gcggtatcct ttatcgtacc gcaacggaac tgggggcgac gaagatcgcg
    46501 ttgggtcacc atcgtgacga tatcctgcaa acgttgttct taaatatgtt ctacggcggt
    46561 aagatgaaag gtatgcctcc gaaactgatg agcgatgatg gcaaacatat cgttattcgt
    46621 ccgctggcct actgccgcga gaaagatatt cagcgatttg ccgatgcaaa agcgttcccg
    46681 attattccgt gcaacctgtg cggttcacag cctaacctgc aacgtcaggt gattgctgac
    46741 atgttgcgtg actgggataa acgttatcca gggcgtatcg agacgatgtt cagcgcgatg
    46801 cagaatgtgg tgccgtcgca tctgtgcgat accaacctgt tcgatttcaa aggcatcacc
    46861 cacggttctg aagtggttaa cgggggtgat ctggcgtttg atcgcgaaga gatcccacta
    46921 caaccggcgt gctggcagcc agaagaagat gaaaatcagt tggatgagtt acggctgaat
    46981 gtggttgaag tgaaataacc aggatagcgc ccgatgcgca agcgtatcgg gctactctta
    47041 tggaggccgg ataagacgcg gccagcgtcg catccggcaa tcccgaataa gatgtttact
    47101 cttgcacccg gcaattcaac atttcattat tttaataacc gcacccggca cgtttttcct
    47161 ttaatcttcc cgccctgtaa ctgtttccat gctttatgag caacagcctg acggaccgcg
    47221 acatagacat gcgccggatg cacggcgatt ttgccaatat ctgcgccatc aagcccgata
    47281 tctcctgtca gtgcacctaa tacatcaccc gggcgcattt tggctttttt cccgccatcg
    47341 atacacaacg ttgccatttc tgcttccagc gtcgcaatgg aactattagc tggcggcgtt
    47401 tgccagttaa gttttatctg caacatgtca gaaatgatat tggcccgctg tgcttcttcc
    47461 ggagcacaga aactgatcgc cagaccgcta tttcctgcac gagctgtacg accgatgcga
    47521 tgtacatgaa cttcagggtc ccacgccagc tcaaagttca ccaccagctc aagcgattta
    47581 atatccagac cacgcgcagc aacatcagtc gcgaccagta cacgggcgct accgttagca
    47641 aaacgtacca gggtctgatc gcgatcgcgt tgctccaaat cgccgtgtaa tgacaatgca
    47701 ctttgcccta cttcattcag cgcgtcgcag acagcctggc aatctttttt ggtattgcaa
    47761 aacaccacgc aagaggatgg ctgatgcaag cttaataacc gttgcaacag aggaattttg
    47821 cctttgctgg atgtctcata aaattgttgt tcaatgggtg gcaaagcatc tgttgagtca
    47881 atttcaatcg ccaaaggatc gcgttgcact cgtccgctga ttgcagcgat ggcttccggc
    47941 caggttgccg aaaacagaag cgtctgtcga gatgcaggcg caaaacggat gacatcatca
    48001 atggcatcgc taaatcccat atccagcatg cggtcggcct catccatcac cagcgtattc
    48061 aacgcatcca gtgataccgt gcctttttgc aggtgatcca gcaaacgccc cggcgttgcc
    48121 acgataatat gcggcgcatg ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg
    48181 cacaacgtca aaattttggt atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc
    48241 acctgatccg ccagttcacg cgtaggacac agcactaaag cctgggtttg aaatagcgac
    48301 gcatcaattt gctgtaacaa gccgaggcca aaagccgccg ttttgccgct gccggttttc
    48361 gcctgcacgc gaacatcttt tccggcaagg atcgccggaa gcgcggcggc ctgcaccggc
    48421 gtcatggtta aataacccaa ctcattaagg ttcgtgagtt gggcgggagg caaaacattc
    48481 agggtagaaa aagcggtcac aatctattct cgtggtcatc gacgcaaagt tagcaggcgc
    48541 gtatcctcgc agatctacgc tcacgatgcg acaatttaat cggttcttca tcgggtggtg
    48601 ggtcaggcat gggttgcggg cgagggatcg gatcgggcac tggaacagga tcgccaggaa
    48661 tcggttcagg gacaggaatt tgcaaataaa taagtgtcgt catatttccc tctggtcatt
    48721 gggtggactc ttaaagggta gacgctgata aataacaggc aaaaaaaagc cgactcatca
    48781 aagtcggcgt cgtacgaatc aattgtgcta tgcagtaatt caaaaaagga agtaagacaa
    48841 tatggagcgc aacgcccatc gcttgacgtt gcattcacct gcaagagaga tattgccctg
    48901 aatgggtaga gagtttattg acttcgctca aactttgcgg cgtttttgta tacagacagc
    48961 cggaaaaatt gcttttgtta caaccattta ctacgatgca accataaagc aacaccacca
    49021 ataagaacaa ctaacagaat acaaaaaatt gaaaatccga attgccaccc gccgccaggg
    49081 atcccaccaa ggttgacgcc aaataacccg gtcagaaagg tactgggtaa aaagaccatt
    49141 gccatcaacg acattgtata ggtacgacga gctaaatttt cctgcatcac ctgagcgatt
    49201 tcatccgcca tcacgccagt ccgtgctata caggcgtcga tttcgtcaag gccgcgccca
    49261 aggcgatcgg caatatcctg catccgacgg cgttggtcat cgctcatcca cggcaaacgt
    49321 tcactggcaa gacgagcata aacatcacgt tgcggtgcca tatagcgacg catcacaatt
    49381 aattgtttgc gcagcagagc caggaatcca cgcggtggaa tttgctgatc aaggagatta
    49441 tcttcaaggt cgataatttt atcgtgcagc tgctcgataa attcactgga atgatcggtc
    49501 aacgcatcgc acacatccac cagccatccc ccgcaatcgg tcggacccgt gccctcttcc
    49561 agatcgctca ccacatcgtc cagcgccagc actttgcgtt gtcgggtcga aacaattaac
    49621 cgcccgtcca tatatacacg catggcgacc agttgatcgg ggcgttcatc ggtgctgccg
    49681 tttatacagc gcaatgtaat cagcgtgcct tcaccgagac ggctgactcg gggacgcgtg
    49741 ctctcgcccg ccagcgcatc acgtacgtta ttgggaagca gcggtgttgt cgccagccat
    49801 tgggcgctat catggtgtac ataattaagg tggagccaac agggatgcgc ttcatcaatc
    49861 acatctgtat tttccagcgg tttaacgccg cctctaccat ccagcatcca ggcaaatact
    49921 gcatccggga cattaacgtc cgatccctta atcgcttcca cagtgcctcc atcatcaacg
    49981 cattattttg tagtctagcc ttctggccct gttacgcaac atctcatcac cccattaccc
    50041 tgaaatgatt aataaaattc tgtctaaatt gaatacaaaa agcaaaatgc ttttccgtat
    50101 acaaaccgtg tgaagtgtta aatagcgtct atcattatca gaattatctg atcatatgac
    50161 gtggcttttt tgcgatcgga tagcaacaaa aattgataaa aataacggga tctcaatgat
    50221 tacgcacaac ttcaataccc tggacttact caccagtcct gtctggatcg tttcgccctt
    50281 tgaggaacag ttaatttatg ccaatagcgc ggcgaaactg ttgatgcaag acctcacgtt
    50341 tagtcagcta cgaaccggac cctattccgt ctcctcacaa aaagaactgc cgaaatacct
    50401 ctccgatctg caaaaccaac acgatattat cgaaatcctc actgttcagc gtaaagaaga
    50461 ggaaacagca ttgagctgtc ggcttgtttt gcgaaagctg acagaaacag aaccggtgat
    50521 tattttcgaa ggtatcgaag cgccggcaac gctgggttta aaagccagtc gctcggcaaa
    50581 ttatcagcgc aaaaaacaag gtttttatgc gcgctttttt ctgactaact ctgcaccaat
    50641 gttgttgatt gacccgtcac gagatggaca aatcgtcgat gctaacctcg ccgcgctcaa
    50701 tttctatggt tataaccatg aaacgatgtg ccagaaacat acctgggaaa taaatatgct
    50761 cgggcgtcgc gtcatgccta tcatgcatga aatctcgcat ttacccggtg gtcataaacc
    50821 tttgaatttt gttcataaac tggcggatgg ttcgactcgt catgtgcaga cctatgccgg
    50881 accgattgaa atttatggcg acaagctcat gttatgtatt gtgcatgata ttactgagca
    50941 aaaacggctg gaggagcagc tggaacatgc tgctcaccat gacgcgatga ccggattact
    51001 gaatcggcga cagttttatc acattacgga accaggccaa atgcagcatc tcgccatcgc
    51061 tcaggattac agcttgttgc tcatcgacac cgatcgtttt aaacacatta acgatctcta
    51121 tgggcattct aaaggtgatg aggtgttatg cgccctcgcc cgcaccctcg aaagttgcgc
    51181 tcgcaaaggc gatttggtgt ttcgttgggg aggcgaagag tttgtcttat tgctaccaag
    51241 aaccccactg gataccgcgc tttcgctggc tgaaactatc cgcgtaagcg tggcaaaagt
    51301 gagtatttcg ggcttaccac gctttaccgt cagcattggt gtggcgcatc acgaaggaaa
    51361 tgaaagcatc gatgaactgt ttaaacgcgt tgatgatgct ttgtatcggg cgaaaaatga
    51421 tggacgcaac cgcgtgctgg cggcataagc cgcggatgcg tctcgagatc aacgactgcg
    51481 cttagcgtgg cgctcccagt tttcttgctt cgcctgcgcc gttttacgta gtgcgacgta
    51541 acacgccccg ctgccgccat gatgcggtag cgcggtgcaa tatgcctgaa catcatcaaa
    51601 ttcggtcagc cagcgcgcca catagctgcg gacaatattg gcatgcgatt tatcatcccg
    51661 ccctttacca tgaataatca gcacgttacg caaaccatcc gccagggctt gttgaataaa
    51721 actgaacacc attttgcggc actcttccac cggctggcgc aaaagattca ggctcgcctg
    51781 ttgcggatat ttaccactgc gcagcttatc cagcacccca tgttgcaacc cttcccgccg
    51841 aaactccagc ggctgactta gtgggatgat gtcgagaaat ccggtggtga ggaaattatc
    51901 aagctgcagc gtgtcgatac gctgcggcgc acgttggtta cgcgttggat gccagtggac
    51961 atcggtagca cgtttcagcg gctggacatc ttccatggcg tcaagaaaca gcgatttgtc
    52021 gtcaaggttc atgttacatc ctcccgcaat taagagcgcg atatgataac cagaccgggt
    52081 cggtccaaca acgtattacc caaatttcca gtaataagtt ccaaatattg ccgatatttt
    52141 aagcaaaata cttatgcatg attattcatt cacgatatta ataatgtaac ttatattttc
    52201 gtgaaatctg tcactgaaga aaattggcaa ctaaaggtta aaaccgttat aacacagtca
    52261 ccggcgcaga ggagacaatg ccggatttaa gacgcggatg cactgctgtg tgtactgtag
    52321 agtctggcgg atgtcgacag actctatttt tttatgcagt tttaactttg cagatagccg
    52381 cattctcgcc ggaattgcgt gattaatgac gcggtcagtg gtgtctggcg actatcacgc
    52441 cgctgaatca aataataggc cgctttcggt aaaatttcgc taaccggcaa catcaccagc
    52501 ccctgtccgt gcaagggatc gcagcccatt tcttcaggca gtatgctgag aaaatcgctt
    52561 tttgccacca gactgataca ggctgagaac gtctcgcaga ctacaccgac ctgtggcgtt
    52621 tgcgcctgat cgtcaagcaa ttcactcaac tgtttgtagt agctgccgtg tggcgtcggc
    52681 attgtccagc tgtaatccag taactgtttg atcgaacggg caccaatggc ggggtgtccc
    52741 gggcggcaaa agatcgcgaa ttgcttttcc agtaatttct caaaagtaaa ttcgtggtcg
    52801 tacggtccct gataataggt attgatggtg aaatccaatt ctccctgacg caattcatta
    52861 atcatcgaca ccagttgccc ttccataatg cgtactttta cctgcggatg ctgctgatga
    52921 aaacgagata tgacagctgg catcagactg cgggaaatac tggcccccat gccgatatta
    52981 atctgccctg ccagttgccc ttgtcgttgg cgaatatcct cttgggctgc gcgcagctct
    53041 tcaagaatta gactggcgtg ctgataaaaa ctttcaccgg catcagttaa cgtcacgcct
    53101 ttactacggc gaaaaaagag ttgcgccgct aacccttctt ccagctcctg aatagattta
    53161 ctcagtgccg gttgcgacat attcaacatt cggctcgctc cgcgaatgct gccctgacga
    53221 gccacttcaa caaaagcccg aatttgatga atttttacct gaaaagccat gacgccaccg
    53281 ataaccgtta tttatcagac caaagaaact ggcatctact ttaatgcaga tgattgtgtc
    53341 agggtaattt atgaacggtt aaaactgtga aaaatcagtt agtgataagt aaaaactatc
    53401 gctacgtgaa ccgggtcaca ctttttactg atgacgggaa aggttatgga gtctttgaat
    53461 caatttgtta attcgcttgc cccaaaatta tcgcactggc gacgtgattt tcatcactat
    53521 gcagagtctg gctgggtgga attccgcact gccacccttg ttgcggaaga attgcaccag
    53581 ctcggctatt cactggcgct gggtcgcgaa gtagttaatg aaagtagccg gatgggatta
    53641 cctgatgaat tcactctaca acgcgaattc gagcgcgctc gtcaacaggg tgcgctagca
    53701 caatggattg cggcttttga aggtggtttc actggtatcg tcgccaccct ggataccggt
    53761 cgccccggtc cggtgatggc tttccgtgtc gatatggacg cgctggatct cagtgaagag
    53821 caggatgtca gccatcgccc ctaccgcgac ggttttgcgt catgtaacgc cggaatgatg
    53881 catgcctgtg gtcatgatgg acataccgcc attgggcttg ggctggcgca tacccttaaa
    53941 cagttcgagt ccggactaca tggcgtcatc aaactgattt ttcagcctgc agaggaaggt
    54001 acgcgtggcg cgcgggcgat ggtcgatgca ggtgtcgtag atgatgttga ttattttact
    54061 gccgtgcaca ttggcactgg cgtacctgcg ggcaccgtgg tgtgcggcag tgataatttt
    54121 atggcaacca ccaaatttga cgcgcacttc accggtaccg ccgctcacgc aggcgcaaaa
    54181 ccagaagacg gtcacaatgc cttgttggcg gcagcacaag ccactcttgc actgcatgca
    54241 atcgccccgc acagcgaagg agcttccaga gtaaacgtgg gcgttatgca ggcaggaagc
    54301 ggtcgtaacg ttgttcctgc ctcggcgttg ctgaaagtgg aaacacgcgg ggccagcgac
    54361 gtcattaatc aatatgtttt tgaccgtgca caacaagcga ttcagggcgc agcaaccatg
    54421 tatggtgtcg gcgttgaaac tcgtctgatg ggtgcagcta ccgccagttc tccttcgccg
    54481 caatgggtcg catggttgca aagtcaggcg gctcaggtcg cgggggtcaa tcaggccatt
    54541 gaacgtgttg aagcgcctgc gggttccgaa gatgccacat taatgatggc ccgcgtgcag
    54601 caacatcaag ggcaagcctc ctacgtggtg tttggcacac agctggcggc aggtcatcac
    54661 aacgaaaaat tcgattttga cgagcaggtt ctcgctattg ccgtcgaaac gctggcgcgc
    54721 accgcgctca attttccctg gacgcgaggt atctgatgca ggaaatctat cgttttatcg
    54781 acgatgcgat tgaagccgat cgccaacgtt ataccgatat tgccgatcaa atctgggatc
    54841 atccagaaac acgttttgaa gagttctggt cagcggagca tctggcttcg gcgctggaat
    54901 ctgcaggctt caccgttacc cgcaacgtag gcaatatccc aaatgccttt attgcttcgt
    54961 ttggtcaagg caaaccggtt atcgccctgc tgggagaata tgacgccctg gcaggtttaa
    55021 gtcagcaagc tggttgcgcg caacctattt ccgcgacgcc cggtgaaaat ggtcacggtt
    55081 gcggacacaa tttgctggga accgccgcct ttgccgctgc aatagccgtc aagaaatggc
    55141 tggaacaata tgggcaaggc ggcacagtgc gcttttatgg ttgtcctggc gaagaaggcg
    55201 gctcgggtaa aacgttcatg gtccgcgagg gggtatttga tgatgtggat gcggcactca
    55261 cctggcaccc ggaagccttt gccggcatgt tcaatacccg cacgctggca aacattcagg
    55321 catcatggcg ctttaaaggg atcgcagcac atgccgcgaa ttcccctcat ttgggacgca
    55381 gcgcccttga tgccgtaacg ttgatgacca ctggcaccaa cttcctcaac gaacatatta
    55441 ttgaaaaagc gcgcgtacac tatgccatca caaatagcgg cgggatctca cccaacgtgg
    55501 tccaggcgca ggcagaagtg ctttatctta tccgcgcccc cgaaatgacc gacgtgcagc
    55561 atatttatga tcgggtcgcc aaaatcgccg aaggtgcggc attgatgacc gaaaccacgg
    55621 ttgaatgccg cttcgacaaa gcctgttcca gttatctccc gaatcgcacc ttagaaaatg
    55681 ccatgtaccg agccctatcc cattttggta ccccggaatg gaactccgaa gaactggctt
    55741 ttgcgaaaca aattcaggct acgctcacct ccaacgatcg gcaaaacagt ctgaataata
    55801 tcgccgcaac cggtggcgaa aacggcaagg tttttgcact acgtcatcgt gaaacggtac
    55861 tggcgaatga agtcgctcca tatgccgcca ccgataacgt gcttgcagca tcgacagatg
    55921 tcggcgacgt cagttggaaa ctgcctgttg cccagtgttt cagcccctgt tttgccgtcg
    55981 gtacaccgct acatacgtgg caactggtta gccaggggcg aacatctatt gctcataaag
    56041 gaatgctact cgcggcgaaa actatggctg caaccacagt caatctcttc attgattcag
    56101 ggctattgca agaatgccaa caagagcatc agcaagttac ggacacgcaa ccgtatcact
    56161 gccctatccc gaaaaacgtg acaccgtcac ctttaaaata acaacaacgc aaacacaaca
    56221 accgaggaat gtccatgagt atgtcatcca taccgtcatc ctcccaatcc gggaagctct
    56281 atggctgggt cgaaagaatt ggtaacaagg ttccccatcc ttttttgctc tttatctatt
    56341 tgattatcgt actcatggtg acgacggcaa ttttgtcggc ctttggcgtc agtgcgaaaa
    56401 acccgaccga tggtacgccg gtcgtggtga aaaacctgct cagtgtggaa ggattacact
    56461 ggtttttacc caatgttatt aaaaacttta gcggttttgc cccacttggc gcgatcctgg
    56521 cgctggtttt aggtgccggt ctggcggagc gcgtcggctt actgccagcg ctaatggtta
    56581 aaatggcatc gcatgttaat gcccgctacg ccagttatat ggtgctgttt attgcttttt
    56641 tcagccacat ttcttccgat gcggcgttag tgatcatgcc accgatgggt gcgctgattt
    56701 ttctggcggt gggcagacat ccagttgcag gtttactggc cgccattgca ggcgtaggtt
    56761 gcggctttac ggctaattta ctgattgtca caaccgacgt gttgctgtcg gggatcagca
    56821 cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag tgtaattgat aactggtatt
    56881 ttatggccag ctccgtagtc gtactgacga ttgttggcgg cctgataacc gacaaaatca
    56941 tcgaaccacg gttaggtcaa tggcagggaa acagcgatga gaaactgcag acattgaccg
    57001 aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc gctacttttt attgctgcga
    57061 ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga tccgattaat cacaccgtga
    57121 tgccatcacc ctttattaaa ggtatcgtgc cactgatcat tctttttttc tttgtggtct
    57181 cgctggctta tggcatcgct actcgcacaa ttcgacgtca ggcggattta ccgcatttaa
    57241 tgattgaacc gatgaaagag atggcgggat ttatcgtgat ggtttttccc ctcgcccagt
    57301 ttgtcgccat gtttaactgg agcaacatgg ggaaattcat cgccgtgggg ctgaccgata
    57361 tactggaaag ttcagggctt agcggcatcc cggcgtttgt cggtctggcg ttgctttcct
    57421 ctttcttatg catgtttatc gccagcggtt ccgcaatctg gtcgattctg gcccccattt
    57481 tcgtaccaat gtttatgcta cttggctttc acccggcatt tgcgcaaatc ctctttcgta
    57541 ttgccgactc atccgtattg cctttagcgc cagtatctcc ttttgttcca ctgtttcttg
    57601 gattcctgca acgctacaaa ccagacgcga aactgggtac ttactattcg ttagtcttgc
    57661 cctatccact tatctttttg gtggtatggc tgctgatgtt gctggcgtgg tatcttgtcg
    57721 gtctgccgat aggtccgggt atttacccac gtttgtctta agagagaacg gatgctgaga
    57781 ttacttgaag aaaaaattgc cacgccactg ggtccactgt gggtgatttg cgatgagcaa
    57841 tttcgcctgc gggcggttga atgggaagag tacagcgaac gcatggtgca gctgctggac
    57901 atccattatc gcaaagaagg ctatgagcgc atttctgcca ccaatccagg cggtttaagc
    57961 gacaagcttc gtgaatattt tgccggtaat cttagcatta ttgatacgct tcccactgct
    58021 acggggggga cgccatttca gcgcgaagtc tggaaaacac tacgcactat cccctgcggg
    58081 caggtaatgc attacggcca actggctgag caattgggcc gtcctggcgc ggcgcgtgcc
    58141 gttggtgcgg caaacggatc gaatcccatc agcatcgtcg taccttgcca tcgggttatt
    58201 ggccgaaacg gcaccatgac cggatatgca ggcggagttc agcgaaaaga gtggttattg
    58261 cgccatgaag gttatctttt gctgtaaaca ttaaacaatt tgtgccagct tgttcacact
    58321 tttatgtaaa gttaccctta acaacttaag ggttttcaaa tagatagaca tatatttaca
    58381 tctaatatcg gaattctctg ctgttaaggt ttgcttagac ttacttgctc cctaaaaaga
    58441 tgttaaaatt gacaaatatc aattacggct tgagcagacc tatgatcccg gaaaagcgaa
    58501 ttatacggcg cattcagtct ggcggttgtg ctatccattg ccaggattgc agcatcagcc
    58561 agctttgcat cccgttcaca ctcaacgaac atgagcttga tcagcttgat aatatcattg
    58621 agcggaagaa gcctattcag aaaggccaga cgctgtttaa ggctggtgat gaacttaaat
    58681 cgctttatgc catccgctcc ggtacgatta aaagttatac catcactgag caaggcgacg
    58741 agcaaatcac tggtttccat ttagcaggcg acctggtggg atttgacgcc atcggcagcg
    58801 gccatcaccc gagcttcgcg caggcgctgg aaacctcgat ggtatgtgaa atcccgttcg
    58861 aaacgctgga cgatttgtcc ggtaaaatgc cgaatctgcg tcagcagatg atgcgtctga
    58921 tgagcggtga aatcaaaggc gatcaggaca tgatcctgct gttgtcgaag aaaaatgccg
    58981 aggaacgtct ggctgcattc atctacaacc tgtcccgtcg ttttgcccaa cgcggcttct
    59041 cccctcgtga attccgcctg acgatgactc gtggcgatat cggtaactat ctgggcctga
    59101 cggtagaaac catcagccgt ctgctgggtc gcttccagaa aagcggcatg ctggcagtca
    59161 aaggtaaata catcaccatc gaaaataacg atgcgctggc ccagcttgct ggtcatacgc
    59221 gtaacgttgc ctgatttttc cgcataactc actatccttc tgtcatatca ttaaattttt
    59281 ctgatttatt gatctggcag aaggttcatc actgtttcat tcaccagata tgggttaatc
    59341 ttttaattac aaactgcgtt gacagttgtt gtaaggagac cctgtatggc tatgtatcag
    59401 aacatgctcg ttgttatcga tcctaaccag gacgaccaac cagcattgcg gcgagctgtt
    59461 tatttacatc aacggattgg tggcaaaatt aaagcctttt tgccgatcta tgacttctca
    59521 tacgaaatga ccaccctgct ctccccggac gaacgtaccg ctatgcgtca gggcgtcatc
    59581 agccagcgta cagcctggat ccacgagcag gcaaaatatt atctcaatgc tggcgttccc
    59641 attgaaatta aagtggtctg gcataaccgt cctttcgaag ccatcattca ggaagtgatc
    59701 agcggcggac acgatttggt gctaaaaatg gcgcaccaac atgaccgtct ggaagcggtg
    59761 atttttacgc caacggactg gcatctgtta cgcaaatgcc caagcccggt gtggatggtg
    59821 aaagaccagc cgtggccgga aggaggcaag gcgctggtgg cggtgaatct cgccagtgaa
    59881 gagccgtatc ataatgcgct caatgaaaaa ctggtcaaag agacgatcga actggcagaa
    59941 caagtcaacc ataccgaagt tcatctggtt ggcgcttatc cggtaacgcc aattaatatc
    60001 gcgattgaac tgccggaatt tgacccgagc gtttataacg atgccattcg tgggcaacat
    60061 ttgctggcaa tgaaagccct gcggcagaaa ttcggcatta atgaaaacat gacgcacgta
    60121 gaaaaaggtc tgccagaaga ggtgattcct gatttggcgg agcacttaca ggcggggatt
    60181 gtggttctgg gcacggtagg acgcaccggt atttcagcag cattcctcgg caacacggcg
    60241 gaacaggtga ttgatcatct tcgctgcgac ctgctggtca ttaaacctga ccagtatcag
    60301 acacccgttg aactggatga cgaagaagac gattaacgac tcttcaataa ctgacaaggc
    60361 ccgagttatc gggcctattt tttacttact gctcttcgcc gccaaggaaa taaattccca
    60421 acgggattgc cagcaacaca gtaaagacca ggctataaac aaaaatcatc gatgactgaa
    60481 tcacatacat tgatgtcgtc cactcagaca gcggaatatt gtactgttct ataacgccgc
    60541 cgatggtcgc gcgagtcaca accgacgccg caataatgaa aaccgccaac aaaccaaaga
    60601 gaaatttctt ccctttcgct gacttcagtt tcgccataat catgatgtaa tccttgtgca
    60661 gacaaatttc ttgttaccta aacgtaacaa taccatgaca tttcaccgtt gtctgctctg
    60721 aaaacctcac ctcttagctg gatttacaat caacgcattg attagttagc taatgttgct
    60781 acttccttcc ctgactattg atgatctgaa tgaattgtcg cggtaaaata gccccgccag
    60841 gggtttccgg catgacgaca attatctgga ggagcctgat aaaacaatga tcgctgaact
    60901 gtttacaaat aatgcgctta atctggtcat tattttcggt agctgcgcag cattgattct
    60961 gatgagcttt tggtttcgcc gtggaaatcg taaaagaaaa ggatttttat tccatgcggt
    61021 gcaattttta atctacacca taattatcag tgctgttggt agcattatta attatgttat
    61081 agaaaactat aaactcaaat ttatcacccc aggcgttatc gattttatct gtacgtccct
    61141 gattgcggtt attctgacga ttaagctatt tctgctgatt aatcagtttg aaaaacagca
    61201 gattaaaaaa ggtcgcgata tcaccagtgc gcggattatg tcgcgtatca tcaaaatcac
    61261 cattattgtg gtgcttgttc tgctttatgg cgaacatttc ggcatgagcc tttctggctt
    61321 gctgaccttt ggtggtattg gtggtctggc tgtcggtatg gccggtaaag atattctgag
    61381 taacttcttt tccgggatta tgctctattt cgaccgtcct ttcagtattg gcgactggat
    61441 ccgttcaccg gacagaaata tcgaaggtac agtagcggaa attggctggc gaattaccaa
    61501 aattacgacc tttgataatc gtccattgta cgtaccgaac tcgctgtttt cgtcgatcag
    61561 cgtagaaaac ccaggacgaa tgaccaaccg ccgcattacc acgaccattg gtttacgtta
    61621 tgaggatgcg gcaaaagtgg gcgttattgt cgaagctgta cgtgagatgc tgaaaaatca
    61681 cccggccatc gaccagcgac aaaccttact ggtttatttc aaccagtttg ctgactcttc
    61741 attgaatatt atggtttatt gctttaccaa aaccacggta tgggctgaat ggcttgctgc
    61801 acagcaagac gtttatttga agattatcga tattgtacag tcacacggcg cggactttgc
    61861 cttccccagc cagacgctgt atatggataa cattacaccg ccggaacagg gtcgctaaat
    61921 cagacatcac tggcgttatg cacaagggta acgccagttc ccatcacatc aatgcttcac
    61981 aatatacata gtccgactgt acgccacatc ttcaggatta ttaatgggat aacctttcag
    62041 ccacggcttg attaatcgtc cattggtata ttgataaatt ggtgcaatcg gtgcttgctc
    62101 catgaggatt ttttctgccg cgttgtaatc ggcattacgc gctttaacgg tattttccgt
    62161 cgatgcctgg gccagaactt tgtcatatgc cggattgtta aagcgtgaaa tatttcctga
    62221 atgcgttgac gttaataatg tcaggaaagt ggagggttca ttataatccc ccacccacga
    62281 ggcgcggata acatcaaaat tgccggtgtt acggctatcg atataggttt tccattcctg
    62341 attttgcaat ttaacatcta cgccaaggtt ctttttccac atcgatgcta cagcaatcgc
    62401 aattttttga tggttttctg aagtgttata caaaagcgtc agcttcagcg gtttttgcgg
    62461 accataacca gctgcgctca acaaagtttt tgcctgcgca ttcagttctt cctgactcat
    62521 ttgttcaaac ggcgaaggtt ccggcgtaaa tcccgcggta acatctggtg taaaatgcca
    62581 cgctggcttt tcgcccgtcc ctaatacttt ttcggtcatc aggcggcgat ctatcgtcat
    62641 acttaatgcc agacgaacgc gctgatctgc cgtcggccct ttttgcgtgt taaacgcata
    62701 ataataggtc ccgagctgcg gcggcgtata aacctgcccc ggaatatcct tcaacagctt
    62761 ctgatacata tttttcggga aggattcggt gatatcaata tcccccgcga ggtaacgctt
    62821 agtggctacg gattcctgat taattggcag gaaggtcact ttttgcagta ccgttttggc
    62881 gttatcccaa taatgggtat tcggtaccac gaccagtttt tcattgacta cgcgctcttt
    62941 aagaacataa gcgccattgc cgatcagatt tccgggtttc gtccactctt taccgctttc
    63001 tacgttggct ttttgcaccg ggaagaaggc aaagttagcg gttaaattca caaaccacgg
    63061 caacggttta tcaagctgaa ttttcaaagt atgggcatca actgcggtga cgccaagctg
    63121 gtcaggcgta gctttaccat caataatcgc ctgtgcgttg ttgattcccg ccagcgcggc
    63181 aaaccatgca aatggcgaca atgtttttgg gtccaccaga cgttgccagc tgtagacaaa
    63241 atcttgcgcc gttaccggtg tgccatccgc ccattttgcg ttatcgcgca gggtaaaagt
    63301 ccagatacgg ttgtcattac ttttccactg agtcgcaacg ccggggacaa tctccccttt
    63361 ttcgttctga ttcaccagac cttcaaacag atcgcgaatg acctgaatct ctggcaggcc
    63421 cacggcttta gcgggatcca gcgacgcagg ctcatcttta atgtggcgca ccagctcctg
    63481 cttctctgcc agtactgtgc cgctcggaac ttctgcagca tacgaaagag aaatgctgct
    63541 gaccaacagc gcacaacacg tgactgaaac agagtgcttc ataagtttcc ctctaaccga
    63601 cattaaaatt gatgcgtaat tatttgtttc gccttaaaga aatgcaaaca acttccatca
    63661 aaaaagtgac aaaggaagca tcaaatgttt taagtatcaa tatatcaaat cagatttagt
    63721 gtaaataata gtctccaaaa tgcggtaaag cacatagaac tccgaacact acgttcgggc
    63781 ttcgcacgtt caaacccgtt ttcggtatca taatcagcat ctgtcgccat tgtacgaaga
    63841 ataaaaaatg accaaaacgt tgaaaggaac tgagaatacg ttaagagaag aattaaatta
    63901 atgtatcagc aagtagtgct aaacagcacc tttatacttc agcctatcta taaataatga
    63961 gaatgccgag ccagcatgtc gacggtgtgg atagtacagg tgataaccgg gtaaatctgg
    64021 tgtgaattta tccaaaacac gtatcagttt ttttctttaa tagcccgctc aacctgatca
    64081 taaggtagat acgccaatcc atgcccatca attgcagcat caatgatcag gtctatcgta
    64141 ttcagtaaaa gctgaccttc catgcgaaca cgaacttcac gtccaccgcg tattaatctc
    64201 cagcgatttg ctgtacccga tgtgggaaga tacaaattaa ttgcctgatg atctattaat
    64261 tgtgacactg acgttggaac acttcggcga gaaaaataat ccggtgagcc aacaatagcc
    64321 attggtatat ctggcccgat tcgaatggcg atcatatctt tatccatctc cccacccaga
    64381 cggacgcctg catcaaaacg ttcagaaacg acatcggtca aaccataatc aatggtgagc
    64441 tgaatatcaa tttcaggatg cgatttcagg aatgtgcgca ttgctggtaa caatatcgtt
    64501 tttgctgcat gttctacagt agtaatacgt attgtcccgg atgggcggtt ctgcagatcg
    64561 ctcagggatg ccatggctga atctatgtca tgcaacatag ggccaagaac agacaaaaga
    64621 tgctcgcccg cttcagttgg aacaacgctg cgcgtggttc gcgtcagaag ccgcaatccc
    64681 aatcgttctt ctatacgacg cactatctgg cttaaagctg actgcgccat gctcaggcgg
    64741 gctgctgcac gagtgaagct acgctcctct gcaacgacga caaacgccat cagatcagcg
    64801 atttcttcac gcttcattca gtatctccat gcaatctatt aatcatttaa atgataggtc
    64861 taatcatgat tttgggtcta atcaacttaa tgatcaatat ctatagtgat atcaccaacc
    64921 gttaaaacga accgacaact ccatgaggaa cggaatgatg aataataaag tcagcttcac
    64981 taacagcaat aatccaacca tctctttgtc tgcagtgatc tatttccctc caaaatttga
    65041 tgaaacccgt cagtatcagg caattgtgtt atctcaccca ggtgggggcg ttaaagaaca
    65101 aacggccgga acctatgcca aaaaactggc ggaaaaggga tttgtcacta ttgcttatga
    65161 cgcatcttat caaggtgaaa gtggcggcga gccgcgtcag ttagaaaacc catacattcg
    65221 tactgaagac atcagtgccg tgattgatta cctgacaacg ctttcttatg tcgacaatac
    65281 ccgaattggt gcgatgggaa tttgtgccgg tgcaggatat acagccaatg ctgcaattca
    65341 ggatcgccgc atcaaggcca tcggtaccgt tagtgcggtc aatattggtt cgatattccg
    65401 taatggttgg gaaaacaatg tgaaatctat tgatgccctg ccatacgttg aagcgggttc
    65461 aaacgccaga accagcgata ttagcagcgg agaatacgcc attatgccac tggcaccaat
    65521 gaaggagtcc gatgcgccga acgaagaact gcgtcaggcc tgggagtatt accacacccc
    65581 tcgcgcgcag tatccaacag caccgggtta cgctactttg cgcagcctta accagattat
    65641 tacctatgat gcttaccata tggcggaagt gtacctgact cagccgacgc agattgtggc
    65701 gggcagccag gcagggagca aatggatgag tgacgacctg tacgatcgag catcaagcca
    65761 ggataaacgc tatcacattg ttgaaggcgc aaaccatatg gatctttatg acggcaaagc
    65821 ctatgtggct gaagcaattt cagtattagc tccgttcttt gaggaaactc tgtaatgaaa
    65881 catattcaaa tccgcaatag tgatatggcc tggcatattg ctgccaataa tctgggataa
    65941 ttttcatgaa aaatgtactg attcttggtg ctggcggtca gatagcccgc catgtgatta
    66001 atcaactggc agataagcaa accattaaac aaactttatt tgcccgtcag ccagcaaaaa
    66061 tccacaaacc ctatccaact aataaaatgc aaacaacttc cggcaaaaaa gtgatacaag
    66121 gtcgatgaca tagcgttgaa gaaaaggatt tgataatccg caaaatttgc acaacactgc
    66181 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
    66241 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
    66301 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
    66361 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
    66421 gccgtcatca tgtggtgctg tgtgtgaatc ctgacggctg ccagttgggg ttacgggcca
    66481 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
    66541 ccgtttatcg ctggaacagc gccgctgaag aacgcgatgt cgtattactg acaggtgaca
    66601 aacctggctc cgaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
    66661 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
    66721 taggcgaatg gctggctcag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
    66781 cgcctggctc tttcggcagt tggtgtgcag atctgaactt gcattgcatc accgcagagt
    66841 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
    66901 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
    66961 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
    67021 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
    67081 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga cccccggttt tatcgagctt
    67141 aatattaacc atctcatagc gccctttcag cgccttcaaa ttgctacgag tatgacaact
    67201 ttcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
    67261 aggaagcggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
    67321 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
    67381 tgtgcgaatt gccaccatcc gctcactgat aagatggtta tccagcttca ctttcagtaa
    67441 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
    67501 gacaaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
    67561 cgattgctgc tgtcttcgcg cgaccagatc ccacaatgca caatccagcg cattacgtgc
    67621 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctagttgcgg
    67681 cacgacgctc ataatttgcg ccattaccga ggcatcactt tccccataac gcggatacgg
    67741 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
    67801 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcgaa
    67861 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
    67921 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
    67981 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
    68041 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
    68101 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
    68161 tattgatacc ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg caactgagat
    68221 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
    68281 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
    68341 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
    68401 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
    68461 cgagccggat tacgaagcag ctctggctgt actgaaagca taatttaata tgcctgatgg
    68521 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
    68581 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
    68641 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
    68701 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
    68761 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
    68821 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
    68881 aggtcagcgg ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac
    68941 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
    69001 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
    69061 agattcttct gcgtagcgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
    69121 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
    69181 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
    69241 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
    69301 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
    69361 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
    69421 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
    69481 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
    69541 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
    69601 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
    69661 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
    69721 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
    69781 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
    69841 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
    69901 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
    69961 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
    70021 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
    70081 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
    70141 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
    70201 ggcgtaaaga tatccggtca gcacccgata ttgcattatt tttcgctggg cgttttgcct
    70261 ttttgcagcg tttctttcac ctgaccgata agctgacgac ggaaatcccc gaggcgaggt
    70321 ttgtcatcgt caatccacgg cagcgggcgg caaagctcca tagctttaat cccgagtcgt
    70381 gccgtcagaa gtcctgcacc aatcccctga gctgcgcggg tagacaaacg agcagcgaga
    70441 tcttgcgaca tccagtccat ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg
    70501 atattcagca ataccagctt aaacagacgc aaacggctgt aataccccag ttcaatgcca
    70561 tacagcgtgg cgatgcgatt aattaaacgc agattgcgcc aggcgataaa cgccatatcg
    70621 accaacgcca gcgggctgac cgcaatcatc aacgttgatt ccgccgccga acggctgatt
    70681 tcgcgccgcg cttgggcatc taaaactggc tggaccaaat gcgcatacag gctgaccact
    70741 tcacggtcgt tttgcgtttc atggattgag gcataccagc gttgcagcgc cggatgcgac
    70801 tgatcaatac ccgcctgctg cgccagtttt tcgcaaaatg cgcggccttt gcccgtgccg
    70861 tggctatgca ataaatcacg cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat
    70921 aagcgccgcc actctgttac cacagaacct acgccagcgc cgataatcaa tgccccagcg
    70981 gcacatccac ccagcgccac ccagtcctgg gtttgccagg cattcattgt ccactgtaca
    71041 ccctgcccga caacgcttgc gccaaacaga gccagccctc ccatcaccat tttgcgccac
    71101 aggctgcgtt tcggacgtaa cgctgcgtcc attaccgctt cgacttgccc ctcttcttcc
    71161 tgcgcttcgt cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc
    71221 tgcgccctga atttaggatt ctgatcgacc tccagaggac cgtcgaaatc aatacgtggt
    71281 tttaacggtt cggtcatcgc aatttatctc ctattaaaaa ttccagcgca gcatcaagac
    71341 gaatatgcgg tagtggtttg tcgacatcca tcacctgcgg acgaaatgcc tcaaactgga
    71401 agccttgctt atcccagaac gcctgaccag gcaaacgtgc gggaacttcg ccaggataaa
    71461 cagtgagcgg tgcgccatcg ctaagtcgat taccacgcag cgccgggatt ttctcaccgt
    71521 taacatcaat aatgccgctg gtggtcgcct gaactgacgc cagccccagg cagtccatgc
    71581 tgatcccttc gaacgccgca ttttgccagg catcctgaat cagttgttgc agtaatgaaa
    71641 ccatattagc gtgctgatcg atggtcacat ggtccgcttt agtggcagca aacaatagct
    71701 tatcgataac cggcgaaaac aaacgccgga acagggtacg ctgcccgtag tgaaaacttt
    71761 gcatcagctg cgtcagtgcc agacgcatat cattaaatgc ctgtggccca ctgttgagag
    71821 gttgcaggca atccaccagc acaatctggc ggtcaaagcg cagaaaatga ttcttataga
    71881 accccttcac caccttctcg cagtaataat taaagcgctc gcgcagcatt ccggcattgg
    71941 tatgcttatc ggcctgcgcc agtttggact cgccccaggt atcgacatcc ggccacggga
    72001 agaattgcag cgcgggcgca cctgccatat ctcccggcaa gacaaagcgc ccaggctgaa
    72061 taaagtgcag cccctgctct ttacagtggt ggagataatc ggtccacgcg gcggcaatgt
    72121 ccgccagccg gttttcgtcg gcaggcgcta gcgggtccag cccttcgctc atcattcgcc
    72181 atttcgccga ccattctccg cgctgaccat tgagtaagcc cgtcatctgg cgcgaccagc
    72241 ttaaatagtc ctgcgccagc atcggcaggt cgagcaacca ttcgccggga taatccacaa
    72301 tttccagata cagcgtggag gtatccttaa agtggcgtag cagcgaatca ttcgatttaa
    72361 aacgtagcgc caggcggatt tcactgacgc cgcgcgttgg cgttggccag gcgggtggat
    72421 cgccatataa ctgcgccagc ccttcgtcat aggtgaagcg cggaatgcca aagtcacgct
    72481 ggggaatgcg tttcacgccc agcaggcgct cttcacgcac cgcacttaac agcggcaaac
    72541 gtgctccggc gtgaatatta agcaactgat taaccatcgc ggtgataaac gctgttttgc
    72601 cgctgcggct aagtccggtt acagcgaggc gcagatgtct gtcgacaccc cgattcaaca
    72661 gcgcattgag ttcattttta agtcgcttca ttgctgtcct gtgctgctct gaagaccaga
    72721 aaaaaaatca ttcgtaagga tatgaatgtg ggggccaggc cattgattat caactgcccg
    72781 tgttgtcatc gaccggcgaa ctccataaga tgtgcagcat tcacgttcgg acggagttcc
    72841 tgacctatgt cccctactat ttatgatatt gccagggttg caggcgtatc aaaatccacc
    72901 gtatcacgcg tgctgaataa gcaaactaat atctccccgg aagcgcgcga aaaagtgtta
    72961 cgggccattg aagaattaca gtatcagcca aacaagctgg cccgcgcgct gacctcttcg
    73021 ggttttgatg ccattatggt gatttctacc cgttcgacca aaactacggc gggtaatccg
    73081 tttttctctg aagttttgca tgccatcacc gccaaagctg aagaagaagg tttcgacgtg
    73141 atattacaaa cgtcgcacaa tccggcagaa gatctgcaaa aatgcgaaag caaaattaag
    73201 cagaaaatga ttaaaggcat tattatgctc agttcgccag cggatgagtc attttttgcc
    73261 caactcgata aatatgatat tccggtggtg gtgattggca aagttgaagg tcaatatgcc
    73321 catgtttatt ctgtcgatac cgataatttt tgcgacagca ttgcgttgac cgatgcatta
    73381 attgaaagtg ggcatcaaaa tattgcctgc ctgcatgctc cgcttgatgt ccatgtttcg
    73441 gttgatcgcg ttaatggtta taagcagagc ctggctgcgc ataatattgc agtgcgtgat
    73501 gaatggattg ttgatggcgg ttatacccat gaaactgcat taaaagcagc acggcaatta
    73561 ttaagccagt caccgttgcc tgaggcggtg tttgccactg acagcctgaa attaatgagc
    73621 atttatcgtg cggcagcaga gaaaaatatt gctattccgc agcagttagc ggtggtgggt
    73681 tatagcaatg aaacgctgtc atttatttta acgcctgcac cgggcggcat cgatgttccg
    73741 acgcaggagt taggtcaaca aagctgcgag ttattattcc gcttaatttc tggaaaaccg
    73801 tcaccacaga atattaccgt tgccacgcat atgacgctga aataaatacc agctaccttg
    73861 cctgaatgcc agatgcgatg ctagcgcatc ttatccggcc tacatttata gatcggataa
    73921 agtactcgcg ccgcatccgg caatttagcc cattatcaga acgagtaatt tacgccgaca
    73981 cctgcataat ggaatttatc actgtcgcct tcgtcgtgat cctgccactc aaacgcgtat
    74041 tccagcgaaa cggaaagtcc gttctggaaa tcataaccgt aaaataaacc tacacggtta
    74101 aaatcatggc cttcacgttc aatatcatcc tgccagtccc agttactcca gcgatccagc
    74161 ccaatgcgcg tatacggcgt caccgagtgg ttgccgagcg tcagcggcaa ataggcgcga
    74221 atttcttgcg tggaaaactc accgttattg cggctgtcgt ccatattgaa gccgcgctcg
    74281 agataatagt tcactcgcaa ggcaaccgtt tcgttgaagg tatattgcag acctgtttcc
    74341 gtttcgacac gggtatcagc gtaaccggta gtgttcagat cgttggcaaa tttatacatc
    74401 gacaaccaac cgttgaaacg taaatcgtca gtcagtttca catcccagtc tggcgcgatt
    74461 ttccagcgct gcatattcgc cgtgtcttta cccggttcat caacgtagtg ataaccataa
    74521 ttacggaaac cgccggtcag gccgaaactg aaatcatcgt tttcgaggaa ctgataatgc
    74581 acctccagct ccgggcgatc aaaccacgtt ccacgtttac ccgcgctata atctaccggc
    74641 ccttcctgat aataggccag agcaattctc cacggcccgt tggcggcatt aaaatagact
    74701 gaaggctccg ccagcccatc catatcttcg ccataaccct cgacgttttc tatttcgtac
    74761 atcgcgccga tattaaagtg ccagtcgttc ctttcctcgg cctgtgcgca ggccattccc
    74821 gcacacatca ccagtgcggt acagggtaat aactttttca ttattgttcc ttgtattatc
    74881 ctgggggaat gtcttccccc tgtatttagc gaattgctat ttccgtttct gcatcaaaga
    74941 aatgacattt cgtcatatca aaatgaatag tgatattttc tcctgcatga taatcattta
    75001 acgcaccagc acggaccact aactcgtgcc ccccaaccgt ggtgtagagc ataaattccg
    75061 caccggttaa ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg
    75121 gatgaatatc ttccggtcgt attcccatca cgatgtgctt atgcaaactt tcctgtgttt
    75181 tcagaaccgc taatttctct tcgggaatgg ttaatttaag cgtttccgta acgaatttat
    75241 cgccatcgat cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggaaacaa
    75301 acatattcgc gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt
    75361 ctttcataat cacaatccgc gtcgccatgg tcatcgcttc ggtctgatcg tgggtcacgt
    75421 agatcatggt ggtgttcagt ttctgatgca gcttgctgat ctctgcgcgc atttgcacgc
    75481 gcagcttggc atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgta
    75541 caatcgcccg cccaagcgcc actcgctgac gttgcccgcc ggaaagcgcc cccggcttac
    75601 gtttcaggta ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca
    75661 cctctttggc gattttttgc atcttcagac caaacgccat gttgtcgtaa accgtcatat
    75721 gcggatacaa cgcgtagttc tggaacacca tcgctatatt gcgtgctttg gctggaacgt
    75781 cattcattcg tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa
    75841 tcatgcgcag ggtggtcgac ttaccgcagc ccgacgggcc gacaaacacg atgaactctt
    75901 tatcggcaat ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt
    75961 gaatatgttg taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag
    76021 gccgataacc gcggccaggt gagtgattcc gttgaaggca acagtaattg cgccccggtt
    76081 aagcccgcgc cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc
    76141 tgcgcatctt caatgccgat acatgcctgc ggcggcacgc ccagccctgc acaggcggcg
    76201 agaaagattt ccgggtccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg
    76261 aaaaactcgc gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacagaagcc
    76321 agcccaaccg agatctgctg tgcacggaga tctgccagca aagagcgaat gccgggtaga
    76381 acagcgttga ccgtcaactc gcgtagtgag tggacataga gcagattttt gcgatacgcc
    76441 agttgcgccc tctcctgcga gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc
    76501 cgcagagact catcgcggct gatccctttt agggattcgt taaactgcgc atcaatgctg
    76561 atgccaattt cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg
    76621 attacaccat ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgtttgg
    76681 tagcggtccc attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca
    76741 cggttataag ctgaccgttc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac
    76801 gctgcgcgtc aagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact
    76861 gttgccactg ctcaggtaac gccggattga gatgcaattc accgtcacgc acgctcaccc
    76921 cggcaaaacc ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat
    76981 catcacaact atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat
    77041 aactttgggt cagcaggcca cagcgtgcgg caacaatgcc gtggattgct ttacttaatg
    77101 acgagtcgtg aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgctg
    77161 agaactgctc cggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca
    77221 tctcgttcac ttctgcgcgt gaataatcca gcagtatggt ttgcttcccc gccgccgctt
    77281 tgtatttcgc cagattaatc gccggcttag ccataaacga atcatcctgc ggcaaaacgc
    77341 cgtcgggctg aatttctggc atccatagct ctttgaggaa catttcggcg cgatggataa
    77401 acgcatcgtc gctacagccg aactggcggg caatattcag cgcctgttga acgttgtagc
    77461 gggccatata gctggtgtat gcattattat tgacatgttc ggtatattcg tctggcccaa
    77521 taacatcatg aatttccaga cgatcgttaa ctcttaccgc gcggctaatc cagaactttg
    77581 ccgtctccag aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct
    77641 gccagtattg aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg
    77701 aggccacttt ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt
    77761 cttcgccgct gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg
    77821 ctttctcctg cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg
    77881 tcggatcgct aaacagatga aacggtaaca aaaatacttc tgtatcccag aaaacgtggc
    77941 ctttgtagcc ttcgccggtt aagccttttg ccgcaatgct gctgcgctcg tcgtgggcag
    78001 gcgtcatgat gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcttcgc
    78061 cgccatttac cgtgatacga cgtttctgcc accattgacg ccagttttct gttgatgctg
    78121 caagaagttg gtcgtaactc tgctgcgcgc acatttcaag ctggcgaagc gacgcgctgc
    78181 cccactcgtc taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca
    78241 acgtcattgt ctcgcctgca tgaagctgcg cactggtatg ttgcagtaaa cggcgctctt
    78301 tggcggtata gcattgctgc acatcaccgc tcaccttaca gcaacagctg atggccacat
    78361 cactgcgtcc atcctgggtg gtgtagctcc cctgcatcag atgctgacca aacacccgca
    78421 cctgggtttc gtcgagatgt tggcgaccgt gattggtttg cgtggcgtca atgcctgttg
    78481 aaatcagcac tgacgcgtcg gcgtccagtg gcgtaatagt gatttccagc gcaatgagcg
    78541 gcagttggtc tgccgaaaca aagcgacggc tggcgatggt gtaacctgag ccgttgctgg
    78601 tacgccagac aacattgcgg cgtaattcgc cactggcaaa gtcaagctca cgctgccagg
    78661 cttcgtggga taacgagaaa acctcaccat ttatggcaat ctccatcccc acgacatcag
    78721 gcaggttcac cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat
    78781 acatccctcg cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc
    78841 cgttcccctg cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt
    78901 ctgataacgt tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata
    78961 caacgccgcc accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg
    79021 cttgccctgt acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc
    79081 catttcccgt tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg
    79141 aaacagcgtc gcaccagctt tatcaccaca aaagctgacg ttcataatcg actgttcgcg
    79201 gatgttgagt gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt
    79261 gccaaacagc gaatcttcga cgctgtaagt tgccggatcc cactcaccaa attgaccaca
    79321 gctcttttgc gtgccgatct tttgaaagct atgcgcattc acgcttttca ccgccggaaa
    79381 gcccagcaca tacatcgcag catccagcat atgaatgccg atgtcgatca gcgggccacc
    79441 accctgcagt tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc
    79501 gcgggcggtg gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg
    79561 ttgcgtatcg agcgcaaaac gatggtgaaa gtcgtaggcc agcaccttgc ccagtttgcg
    79621 cgcggtatcg cacatttccc gcgcctgttc tggcgtcatg gcgggcggtt tttcgcacat
    79681 cacatggcag cccgcttcca gtgccatcag ggtatgttcg taatgaaaac ggttaggtga
    79741 gcagacgcta accacatcag gtttcaccgc cagcagcatg gcctgcggat cgtcccacac
    79801 ggatgcattc ccgtattttt ctgccagcgc ctgcgcctgg gaaaggcggc tgtcacagac
    79861 agccaccagt tccagatcgt tgcgggtgca gtagtacgaa gcatgaacct tatccgccac
    79921 ctggcctgcg cctattatcg cgacccgcag cggagagctt gtcattgcac ttttcacaaa
    79981 gacctcttag caggtacgca accaggccaa cgaatcacgg tacgcctggg caggatcttc
    80041 cgcccggata cgcccttcgt acactacata gccctgatag ttatccgcgc gcagctgttc
    80101 aaacagcgcg tggaaatcca gggtgccgct gcccggctgg tagcgatggt tatccgcaat
    80161 atgcacatga ccgagcaggt cgcggttgtc atgcagcgcc tgcgccaggt tatcttcttc
    80221 gatattcatg tgatagaaat cgccgataat ctgtacatgt ttaagatcgt tttcgacgat
    80281 gtaacggcgg gcatcggcga gggtgttgat catatgatcc tgatagcggt ttaacggttc
    80341 gagatacacc acggttccgg tacgcgcggc gacctgttcc agtacgcgca gggaatcact
    80401 caccattttg cggtcgccgt ccaggctacg cggcgaggtc atcggcggta agcggaaggt
    80461 aaacatgccc cacgcagccg gaacgacgat gcctttaccg cccacttctg ccagcgcttc
    80521 gagaatgcgc tcgatctgct ttaagccatt aagacgacgc tcttcgataa agtcgccaat
    80581 ccatccgtca tagccaccgc aggcggtggt caccggtaaa ccggtttctt tgatcgccgc
    80641 tttgacttct tcgatgttgt taaccagcag tttgccgtca atctcaaaac catcgaagcc
    80701 catctctttg atataacgaa atttctccag aatgttttcc ggaaagaacg cctgattctg
    80761 tgtgccgatt ttcatttttt tcattccttt gaattaaaac gtaacgccca ttttgatgct
    80821 ctgttccgga tgctggtcga catactgcat atagctttcc gggctattgg caaaggtcac
    80881 caccgggtcg attaaatctt cgcaattgag ataaccgttc atcagcagtt cccaacaggt
    80941 ttcttcaata cgcttgcggc tccagcgcgg ataatccggg ttcggttcgc tgcacgcgag
    81001 agaagacaat tttggcgtta ttgaaatgcg cttcgcgtcc gaggttaaaa ccttcggcaa
    81061 acggcttggc aaacgcgaca taggagatgg tgccgccata agccagaccg cggagcgccg
    81121 attgcagcgc gtcggcgtag ccgctggttt cgataatcac atcggcaccc tgcttgccgg
    81181 tcagcgtttt gatctcttta cctacatcag tgccgatggg gttaaggcag aaatccgcgc
    81241 cgtggcgacg ggcaatatca cagcgatggg cgataggatc gacgccaatc accacagaag
    81301 cgccagcgcg tttagccagt tggatggcaa tttgaccgat tgcgccaagc cctaccacca
    81361 ccacaaaatc ccctacgcgc acgttggcat cgcgcacgcc gctcatggca aactgcgccg
    81421 ggtcgtagca gacggcgttt ttccaggagc tgccttgcgg cattttgcgc agcttgtagt
    81481 tattcactgc gttaatgatg accgtctcgg agagcgggcc gtagccgcat acgctgtcgc
    81541 ccaccgcgta gtcggtaacg tcgctgccgc actcgataat gtcgccaacc accatgttgc
    81601 caagctggaa tttgccaaac tcaatgccgc gcggcgcatc tgccggacgc ggagtgaaca
    81661 tctgccattc gccgttaaag tcttcatcaa taaacgggct ggcggcgcgg aagtcgacca
    81721 cttccgttcc gtgtttcggt gcgccgaaac gcacgcggat cttcacttca ttagctaaaa
    81781 tcgcccgatc ttcatactca accagcgcag caacacgcgg tgctgtggct actaactttt
    81841 tcatttaaaa ctccttgtaa agaaactggc cgctttagcc cttcacgcca ccggcggtca
    81901 aaccactctt aataaaacgt tccgaaagcg catacataat caccaccggc aatgcggcca
    81961 ccagtgaggc cgccatcatc cgtccccaga tgtaatctgg cgtactgaac agcgcgttca
    82021 ggcccaccgg taaggtgaaa ttgctggcgc tggagaggaa aatcgacgca aacagatagt
    82081 cgttccacgc caccataaag cagtagacga acacggatat cagcccggac atcgccaacg
    82141 gcacggtgat gcggaagata atttgcagac ggttgaggcc atccatcatc gccgcttctt
    82201 cgatctcatc ggggatggtg tcgaagtagc ttttcagcat aaacacggcg gtgggcagag
    82261 tttgcgtcac catggtgatg atcagcgcca tctcagtgtc gtaaatgcca agcgcggtga
    82321 tgattttgaa aagcggcacc accagcaaaa tgccagagaa catatacacc gtgtaaaagc
    82381 tggcgttgat ggtcatccga cctttaaagc gcaggcggga aagcgcgtaa gccccaagaa
    82441 tgccgaggaa taccgccacc acggatgaaa ccacagacac caccagactg ttacggaagt
    82501 agtcgacaaa cgggaaaatc atcgggttaa aaatgtcgac gtaatgctcc agcgtccact
    82561 gctgcggcag cagcgtagga tgcagtgaga tcgcctcttt cgcgcccttg aacgaggtca
    82621 tcagcatcac aaaaaatgga aacagcgtga tgatgagaaa cagcgccagc ccgcagtaaa
    82681 aaccgatgcg actgagtgtg cgtttatttg ttgccattga ggttcacccg tttcctggtc
    82741 agcagaatga cagcgaaaat aatgatgaag agcaccaccg agatcgccgc cgcttttcct
    82801 aaatcattaa aggcaaaggc ggttttgtag agatagacac cgagaatatc gaccttggtc
    82861 gtcagcagat aaacatccgc gaacatgtag aacatccaga tggtgcgcag ggtcaccacc
    82921 gtcgccagga ccggcataat cgcgggcagc gtgacgatac gaaaccgttg ccaggcatta
    82981 gcgccatcca tctctgccgc ttcgtacagc gatttgtcga tggtctgcaa aatcgccaga
    83041 aacgagataa aggcatacgg gaagtagcgc cagatggcga acagcaccac cagcacaaag
    83101 ctactgcccg gattatcgaa ccacagcggt gcctgctcat agagatgcag aagatcgacg
    83161 ccgaggtagt tcacaatgcc gtagccgttg ttgaacatgt atttccaggc aaacaccagc
    83221 gaaatggacg gcgttacgta ggagaggatc accagcgaac gcgcggtttt gcgcaggcgg
    83281 aactcgcggt tgaaaaacat cgccacggcc agcccgagca cggtgctgcc cgccaccacc
    83341 agcgccgtat accagaccgt catccacagc gagtgccaga agccgggatc ggagagaatg
    83401 cgcacatagt tactcacccc aacaaacgtt gactcgatgt tgggattgag cggcagacgt
    83461 aaaaaactga tttcgatatt cgacaccatc ggccacgcca ccagaccgcc cagcagtaat
    83521 aagctgggcg cgagaagcag cagcgcaaac ggcatatcgg aacgacctga aaaaagcctg
    83581 ttcattgcgg attcctgtcc tgggtttagt gctgttcgat cagttcatcc agctttttct
    83641 ggctcgcttg cagcgtgcca gggagatcgg ctttacccac ggtgacgtta tgcaccatgg
    83701 agctcaccac gccagaaccc gtcacatcac ccatgcgggt aaagttttta tcccttactg
    83761 cgccaaagac ctgaatattt ggcagttcac cgattagctg attcggtagt tcacccagcg
    83821 ccttaataac gtcgttgtct ttccaggtgg cggtagtgac aaccgcttta ttcaccggca
    83881 gcgcagcacc tggcgacatc atcacccagt cggcaatgtt gtccgcctgc tccataaagg
    83941 tgacaaattt ttctgctgct tcggtctctt cggtcttttg tccggcggta atggtcagcg
    84001 aggtcaacat gccgtagacc gcagagtttt tctcggttgg caccacgaaa ccgacgtttt
    84061 tcgggtcgcc ttctttaatc acagccggaa ggatataggt ggagtaaatc gccatcggcg
    84121 cggtgccgtt cataaaggcg tctttcactt ccatgatgtc gttagaaccc ggcatggtgt
    84181 tggcagcaag gtcgcggtaa taggtcagcg cctgcatcat ctctggtgta tcaagggtga
    84241 ttttgccttc ggcgttaaag acgttagcct ggttggataa cgcaaactgg gagaaggatt
    84301 gttccgtcag cacgctttct gccgtaggca gcgcaatacc gtatttttta ttcgccgggt
    84361 cattcagttt ctgtgcaacg tccagcagct gttgccagtt tttcggctcc tccagccccg
    84421 cttttgccag cacatctttg cgataccaga tgccgccaat ccaggcgctg acaggaacac
    84481 cggtccatgc gctaccatct tcggtacgca caatacgcag tacgccatcg taaaacgcgc
    84541 cttcaccaac gttgctgatg accgtggcaa ccgccttgcg atcgataagc tgctctttgt
    84601 ccatcacttt ggcgtagtca tggctggttt cgatcacttc cggcagcgaa ccgctacgtg
    84661 aaagagtaat gactttagtg ttataggcat cttcttccac gggcacttgc ttaacggtga
    84721 tgccagggtt ttctttttca aaacgggcaa tcaatttact gataacggcc tggcgctcct
    84781 gctccaccga agaatgcata aattcgatgg atacattcgt tttattttct tctttacagc
    84841 ctgaaatcag ggcgcatgaa accagtgctg ataacagcac gatttttgat ttaatcatga
    84901 gatgttcctt tttatttaat ccacataacc tgccaggggc gcagcgttat ttcattaccc
    84961 aatatatcaa cttcactaat cagatcccga ccatgcagat tagtaatatt tacatgctga
    85021 atatttttac tgacattaaa caatccagtc agacaattac catcagcgtt acttctttga
    85081 atacgcatta cggatgaatt aatcgtatca atggtaaaat tattatccgg atgaaactcg
    85141 ttatggctgc gacgaagtgt aattaaacgc gacaactcat gatataccgc atgccttaat
    85201 gtagcttcat cgttcagttc tcgggttatc tctttactgt gatatttttt acggttaatc
    85261 gcacggttat atccgagttt ttcgacacct gcgtaatcat tacgcgagcc aagaatactt
    85321 tgaatatata tcgctggcac accggggaaa cttaacaaaa tcgcatgggc aaggataaac
    85381 ctggcgcaac gttcttcatc gctactctca cggcggctta acgcatccat ataggtcaca
    85441 tttatttcat acggactgcg tgtaccgtcg ggattatttt tccagtttac taatgcacct
    85501 tcctgctgta acgcctcgac cagctctaat atttcgcttt caggcaacaa gccccgtagc
    85561 gggtttagcc cgatgccatc gtgagaggcg aggaagttaa accaggtggt gttgctggaa
    85621 ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa cgttctgttt ttgcaccgca
    85681 tgcagcacca gtggcggcag cgagaactgg tacaccatat gtgcttcgtc atcgcctgcg
    85741 ccaaagtaag caatgttgtc tttatgcgga acattggtct cggtaatgat cactgtacct
    85801 ggcgcaacgt tatcaataat cgaccgtaac agtttgataa tcagatgtgt tttttccaga
    85861 tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag gcggacatac
    85921 tcagcacctt tcgcaaggta acagagtaaa acatccacca tcgccagcaa cacttcaggg
    85981 ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg gcgcgttgaa
    86041 tgatcgcgca tctggaatgg cgttaataac ggtaacgcac gcgggcgagt gacggcgctg
    86101 agatcggttt gcgggtcaac ggcaataaaa aaatcttcaa aacctggatg ctgttgtaaa
    86161 tagtttttaa accattcact ttttgccgac atatggttgc agacaaaatc aaacattaaa
    86221 tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact ggcgacctga
    86281 tgataatcaa ttaccgaaaa gccatcatca gatgaccatg gataaaacgg caacaaatga
    86341 acatgtgaaa aaatgctttg cagccattga tggtaaaact gattaaatgt gggtaatggt
    86401 tttaaatcat tgctgtgaaa ttgatcggca taggtaatta acacgacatc actttcatcc
    86461 cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg cgttacaagt
    86521 ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccaggta atccgtaatt
    86581 ttctgtttca tgttgccttt ccgtggtagc ggtcccatga aacgagagac tactccgcag
    86641 aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat tttttaccag
    86701 ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg ggatgtaaat aaggtgtgac
    86761 agaaaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct ttgaccttcg
    86821 gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc atctcgctaa
    86881 ggatctcttt tgcttgccct gactggcgtc cggcattgca atacactttc acggtgtcgt
    86941 ttttatccgg aacggcggtg gcaatgcgct ctttcacttc cttcagggga atattgatgg
    87001 ccccctgaac gtgctcttgc tgatactgct ctggaacacg aacatcgatc cagtgttcag
    87061 cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag taagcctttt taaacatgta
    87121 agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta acaatttttg
    87181 cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg acttaacagc
    87241 ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga ttttatcgcc
    87301 cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag cttgcctgct
    87361 aatttgaaac caggctttac cttttgcccg gcctgttgcc agcgagtatt cataactttc
    87421 ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca taacgttcca
    87481 tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc agcaattcgc
    87541 tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca agcgcaaatg
    87601 acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc gacagcacca
    87661 ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg gcgcagacgc
    87721 cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta atgcccgcca
    87781 ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg cctgaatacg
    87841 ttcgcgcatc cgttttgctt catcagccag ttgcgctaat cgctgctgct cactttgcga
    87901 caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg gtaaaacaaa
    87961 cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta ctccttagac
    88021 gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc ttgcttcatt
    88081 ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag ttcggcaaac
    88141 tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc ttccatctgg
    88201 tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt gccactgtcc
    88261 agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat caatgcctgc
    88321 tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt cttcatgcgt
    88381 gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag atcggtcagt
    88441 ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag cagcgccagt
    88501 tcggcttttt cctgccattc aacctcacgc gccgacgctt gttcaatacg gcgagtcagc
    88561 tgtttctttt ctgccaacgc gcgcgccgaa gtagaacgta cttcaaccag tgtatcttcc
    88621 atctcctgga tcatcagacg aaccagtttc tgtggatctt ccgctttctc taacagagcg
    88681 ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat gttgtcctct
    88741 tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt gccaattttt
    88801 tatctttttg atttataaag atctgattga agaatcatca gcaacatgcc aggatgaatt
    88861 agcgaattac actaacaagt ggcgaatttc atcatggcag aatacaaaga taatttactt
    88921 ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc accgctggac
    88981 aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agctgattgc cagccgcctg
    89041 cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc ggcgttaaat
    89101 gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac cggtgcgcaa
    89161 aaacgtcatc cagggagatt tgaacgtgcc gacggcggta cgctatttct tgatgaactc
    89221 gctacggcac cgatgatggt gcaggagaaa ttattgcgcg tgattgagta cggtgaactg
    89281 gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc ggttggtatg cgcgacgaat
    89341 gccgatctcc cggcgatggt caatgaaggc acttttcgcg ctgacctgct cgaccgactg
    89401 gcttttgatg ttgtacaact gccaccactg cgcgagcgcg aaagcgacat aatgttgatg
    89461 gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc tgcctctgtt cccggggttt
    89521 acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat tcgtgaattg
    89581 aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc gcttgatgac
    89641 atcattattg atccctttaa acggcgtccg cctgaagacg ctatcgccgt ttcagaaacc
    89701 acctcgcttc caacactgcc gctggattta cgtgagtttc agatgcagca ggaaaaagag
    89761 ttgctgcaac tcagtttgca acaagggaaa tataaccaga agcgcgcggc tgaattactg
    89821 gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta gcgaagttga
    89881 gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa tcaatacgtc
    89941 gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta ttaatcgctc
    90001 agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat ggtcagcgga
    90061 tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag cccctgcgcc
    90121 agcgcgcgtt gctggatttt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc
    90181 gttaaactct gccgcaatca tagaccccag accgcgtacc gccgcgatgg ccggaacgct
    90241 ttctttggca tcaatcagcg tgtttgtcag acgctgtccc agttgattcg cgcgttcgca
    90301 gagtgattct ttgtcgataa tgttgagcac cgcgtgcgcg gcagccaccg ccagcgggtt
    90361 accggcgtag gtgccgccaa gcccgcccgg cgcgggtgcg tccataatat tcgcgttacc
    90421 gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaaatccgg
    90481 cttatcggcg taatgatcca tggcaaacag cttaccggta cgcgcaaagc cgctttgcac
    90541 ttcatcagca atcatcacaa taccgtgctc gtcgcacagg cggcgaatag cggcaaccag
    90601 ctcttttggc gcaacgttga aaccgccctc gccctgcacc ggttcgaaaa taatcgccgc
    90661 cacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga gggagtcctg
    90721 tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac cagggaacgg
    90781 gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca tatacgtacg
    90841 accgtgaaag ccgccgctaa acgcaatcac gccagggcgt ccggtatggg cgcgagcaat
    90901 tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aacgcggttt tggcctgccc
    90961 gctcaccggg gcaagggcgt tgattttctc cgccagggtg acgtagcttt catacggcac
    91021 aatctgatac gcggtgtggg taaactgttg cagttgctgc tccaccgccg cgaccagatc
    91081 agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtactcgtt
    91141 gccctcaaca tccttcagcg tagcgttttc agccgactgg gcgaagaagt tacacatcac
    91201 gccaaccccg cgcggggtgg cagaaagacg acgctgatgg aattcattgt tgctcatgtt
    91261 tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc
    91321 tgtaatacgc cgcgcccatc gcggtaaacg gtacgcgcag cgtgcgcccg ccggggaacg
    91381 ggtaatgcgg cagattggcg aaggcatcga aacgttcggc gtcgccgcgc agcagttcgg
    91441 caatcaaacg tccggctaga tgagtacagg tcacgccgtg gccgctgtag ccctgcatgt
    91501 aatagatgtt ggtatcgagg cgaccaaact gcggcatacg cgacagggtc agcaggaagt
    91561 tgcccgtcca gcggtaatca attttcacgc ccttcagctg cgggaaggtt ttcagcagtt
    91621 tcggcaccac aaggcgctca acgtcatccg ggtcgcgcgc gccgtagacc acgccgccgc
    91681 cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt
    91741 ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcggtgg
    91801 tgatcacctg cgtgccgcac ggcatgctgc gtttcgccag ttccggctct actttatcgc
    91861 ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacct ttggcagttc
    91921 gcacaacggc tggcgtggtg tgctggatct gcgtcacggc agaaagttca tacacgcgcc
    91981 cgccgttgag gcggatggcg tccgcttcgc caatggcaag gtttagcgga tgaatatgcc
    92041 caccgctgtg atccagcagc gcaccggtat agcgatcgct ggctacttca cggcgaatgg
    92101 cgttggcgtc cagcaattcc agctgtttat tgccgtagcg ttcccagttc tctttctgct
    92161 cttcaagtgt ggcgagctgt ttatcattca tcgccacaaa caggccgccg gggcggtagt
    92221 cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcaccacct tcgaacatca
    92281 tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc
    92341 ggctgtagga gttcacaagc tgtccgccat tgcgcccgct tgcgccaaag ccgatgcgtg
    92401 aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg gaggagagcc
    92461 cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc agcgtgtcga
    92521 atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag
    92581 gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc atgcagggat
    92641 ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg
    92701 ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg
    92761 gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgt
    92821 agcgcctgtt cttctgatgt gaaacgcgtg accaccagca ccggaccgaa aatctcttcg
    92881 cgacttaagg acgcattcgg gtccacatcc acaaagatgg tcgggccgat ggcggcagcc
    92941 agcccggcgt tacggccatc caacaacagt tgccctttgc tttcgccttc ccgaataaag
    93001 ctatggaccg agtcggcgtg ggcgcagtcg attaaggtgc ccatggtggt tgcgggatca
    93061 agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcatcg
    93121 gcgatgctct cttccagcaa caggcgcgtt ccggcgatgc acacctgtcc ctggttgtag
    93181 aaaatgcctg ctgcggtggc gcttgccgcc tgttgcaaat ccgggcagtc agcgaaaacg
    93241 atgttggcgc ttttgccgcc cgcttccagc cagacgcgtt tcatgttgct gtcgcccgca
    93301 tctttcagca gctgtttccc ggtacgggtt gaaccggtaa aggcaatggc gtcgatatcg
    93361 ttatgacgcg acagcgcctg cccggcttca tgaccaaaac ccgtcaccac gttcaacaca
    93421 ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat
    93481 ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc gagtttccag
    93541 caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc gaccggttca
    93601 cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg
    93661 atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc acgcagactg
    93721 tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct
    93781 tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac
    93841 cagtcgccgc gttcaaatac gccgcgtgct gcgctcatcg cacggtcgat atcgacgctc
    93901 ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt
    93961 tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgaga
    94021 cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca
    94081 cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg ggccgtatgc
    94141 ccgtttattt ctggcgacat tctttatata cacttcatcc ttcaagccgc ctctgccttg
    94201 gctgcgttcg ctcccccgaa tcacttacct gagtaagctc atcgggattc gctcacttgc
    94261 cgccttgatg caacttgaat gattttgtgt atagattgcc gggcgcattg cgcttacccg
    94321 gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat
    94381 acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc
    94441 cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtacagt
    94501 gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt
    94561 ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac
    94621 accctgacta cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg
    94681 tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg
    94741 cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt
    94801 cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga
    94861 caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt
    94921 ggtcagagtc gctgtttttc agcgatatgg tgctgacaag cggtgataaa gccctcgaac
    94981 aatatacgcg aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgctcagc
    95041 gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca
    95101 acacgcaacc gtgggctaac gaccttcgcc ccttgtccat gtagagagtt tacccaaaag
    95161 ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc
    95221 gacggtgcat attgctgttc caccggcagt tcgggatctt cccgatgttc cagcaattca
    95281 ggctgttcgc acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcttgtaaa
    95341 ccccggcaga tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat tatcgccatg
    95401 ctcagaagat cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atataggtgc
    95461 ggctgcacat tgctgggact accaggaaga taaatgccat cgagtttcgg caaaagttgt
    95521 tcaagtaatg acggttccgc cagcgcatgt ggtagcgcaa taggcaagcc gcctgcatgg
    95581 atgatggcat tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc
    95641 ctgcacatta cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca
    95701 aagtggacta aattatcgcc attactgcgc tttaaatgac ctttatgttc aatatttttt
    95761 caatctagca gtgggttttc atttttgcaa actcaattta acatttgaca aacatttagt
    95821 ttgcatacag attcgaatgg tggtcattat attttacgct ttgataacga gcggaaaaca
    95881 aaccaaaggc gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag
    95941 tcagaagaga ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac
    96001 ccgaatacgc aatatgttga tgttttactt accgatttaa atggttgctt ccgtggaaag
    96061 cgtattcctg tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggta
    96121 tttgcaatgg atattctggg caacgtagta gaagaagcag gtctgggtca ggaaatgggc
    96181 gagccggatc gtacctgtgt tcctgttctc ggttccttaa ctccttctgc cgccgatcca
    96241 gagtttatcg gtcagatgct cctgaccatg gtcgatgaag aggcgctccc tttgacgttg
    96301 agccgcggaa cgttcttaac cgcctgtggc agcagctgcg ccagcgcgga ttgttcccgg
    96361 tcgtagcggt agagctggag ttctatttac tggatcgcca gcgcgacgct gaagggtatc
    96421 tgcaaccgcc ctgcgcgcct ggcaccgatg accgcaatac acaaagccag gtttactccg
    96481 ttgataacct caaccacttc gccgacgtgc tcaatgatat tgatgaactg gcgcagttac
    96541 agctgattcc ggcagatggc gcggtcgctg aggcctcgcc gggtcagttt gaaatcaacc
    96601 tttaccatac tgataacgtg ctggaagcct gcgatgatgc gctggcacta aaacgcctgg
    96661 tgcgtctgat ggcagaaaag cataagatgc acgccacttt tatggcgaag ccgtatgaag
    96721 agcacgcggg cagcggaatg catatccata tcagtatgca aaataatcgt ggcgagaacg
    96781 tgctttctga cgcggaaggc gaagattcgc cgctgctgaa aaagatgctc gccgggatga
    96841 ttgacctgat gccgtcgtcg atggcgttgc tggcaccaaa cgtgaactcg tatcgccgct
    96901 tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg ccataacaac cgcaccgtcg
    96961 ccctgcgtat tccgtgcggc gaccgtcata atcaccgcgt ggaatatcgc gtggcgggtg
    97021 ccgatgccaa cccatatctg gtgatggcag cgatttttgc cggtattttg cacggtcttg
    97081 ataacgagct gccgttgcag gaagaagtcg aaggcaacgg gctggaacag gatggcttac
    97141 ccttcccgat tcgccagagc gatgccctgg gtgagtttat cgagaatgat cacctgcgcc
    97201 gctatttagg cgaacggttt tgccatgtct atcacgcctg taaaaatgat gagctgttgc
    97261 agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac gcgtaatacc
    97321 ccttgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc tggtaatcca
    97381 gacattttcc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg ttaacgatta
    97441 agactgtgac gaggaaatga gatgatgccg atgattaaat ccccgcatgg cgagggaggc
    97501 tgcgtatgcg cccctcctgc gactgactgg actccgcccc ctttgcttcc cttgctaaac
    97561 aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg cagcaattat
    97621 ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc tgcgaaaatc
    97681 actgaaattg tggcaggtgg tgatgatggg tctggcctat ctcacgccga tgaccgtatt
    97741 tgatactttt ggtattgtct ccggcattag cgacggtcac gttccggcgt cctatttgct
    97801 ggcgctggcg ggtgtgctgt ttaccgctat cagctacggc aaactggttc gccagtttcc
    97861 ggaggccggt tcggcctata cctacgcgca aaagtcgatt aacccgcacg tcggatttat
    97921 ggtcggctgg tcatcgctgc tggattatct ctttttgccg atgattaacg tcttgttggc
    97981 gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg tggtaacctt
    98041 cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta acttcaatac
    98101 cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg tagttcaggg
    98161 actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta tcagcgagaa
    98221 cgcgcacctg atcccgatta ttaccggggc gacgattgtc tgtttctcct tcctcggttt
    98281 cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga tcccgaaagc
    98341 catcttcctg acggcggtct acggtggcgt tatctttatc gcggcgtcgt tctttatgca
    98401 gctgttcttt cccgatatca gccgctttaa agatccggat gccgcactgc ctgaaattgc
    98461 gctgtacgtc ggcggcaagc tcttccagtc gattttcctc tgcaccacgt ttgtgaacac
    98521 gttagcctct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg tgatggggcg
    98581 tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc ggactccggc
    98641 actcaacgtc attatggtcg ggattgtcgc gttgtcggcg ctgttcttcg atttagttac
    98701 cgccacagcg ttaattaact tcggtgctct ggtggcgttt accttcgtga atctgtcggt
    98761 gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc acttccacta
    98821 tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta acctcgagtc
    98881 aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt tatggtatct
    98941 gatccgccgc tatcgcaaag tgccgttgta tgatggtgac agaacgccgg tgagtgaaac
    99001 gtaatagtcg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc gccattaact
    99061 ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc ttagcgcatt
    99121 aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat attgccgatg
    99181 agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg ctggcccact
    99241 acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagcgaa gaagcccaac
    99301 atgaaatggc tgctgaagcg gggattaatc tcgtacgcat tgatgagatc gcagagtttt
    99361 tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcaat atcgttgtcc
    99421 ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg tgctttaatg
    99481 ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt tatagttata
    99541 gagaaatcac gggtacgaat gttgaacaga tggcgacgcc acctgttcaa cgaccaacca
    99601 cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat taaatattag
    99661 taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta tcgtctcttt
    99721 tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc cccccgcatg
    99781 ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac acctttaacc
    99841 catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt tatgatcgac
    99901 tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa agctgggaag
    99961 tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg tttcaaaaaa
   100021 ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt accttccagc
   100081 gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc ccctacttcg
   100141 acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat cataccgttg
   100201 agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc cattatgctt
   100261 cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag gagcaactcg
   100321 accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc gggcaattta
   100381 ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg caggtagtgg
   100441 tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg gaatgcgacg
   100501 ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg cgcttgcgtt
   100561 taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc aaaccgccgc
   100621 taaataatcc cgctgtccgc catgcgctgg cactggcgat taataaccag cgcctgatgc
   100681 aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc gcttcgtggg
   100741 cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc gaacagttga
   100801 agtcgttggg gctggaaaat ttaacgctga aactgtgggt gcccacacgt tcgcaggcgt
   100861 ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg caggttggcg
   100921 taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg atggatatga
   100981 gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac agtttcttcc
   101041 gtccgttact gagctgcgcg gcaattcatt cgcagaccaa cctcgcccac tggtgcgatc
   101101 cgaaattcga cagcgtattg cgtaaggcgc tctcttcgca gcagctggcg gcgcgtattg
   101161 aagcctatga cgaagcgcag agtattctgg cgcaggaatt gcccattttg ccgctggcgt
   101221 cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt agcccgtttg
   101281 gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa aaaccatgat
   101341 tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc tgaccttcgt
   101401 tggcttcagc ttgagctatt tcacccctca tgcgccgtta caaggcgcgt cactgtggaa
   101461 tgcctgggtg ttctggttta acggcctgat ccactgggat tttggtgttt ccagcattaa
   101521 cggtcaaccg attgccgaac agttaaaaga ggtcttcccg gcgacgatgg aactgtgcat
   101581 cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg ccgggattac
   101641 gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct tttcaatccc
   101701 ggtgttctgg ctggcgcttc tgttgacgct gttttgttca ctcacgctag gttggttgcc
   101761 cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt ttgcgttgat
   101821 tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg caatccgcca
   101881 tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga tccgcctgat
   101941 gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg cgacccgcgg
   102001 tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac cgccggttat
   102061 tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca ccgaaatggt
   102121 ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc aggattacgc
   102181 agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta acgtgatttc
   102241 tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg ccttacgata
   102301 gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc aaattttata
   102361 gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg gctggctgta ctgtgtattt
   102421 ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttac caattattgc
   102481 cgccgtcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac gacctcggac
   102541 gcgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc gcatttgtgg
   102601 tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg gcgacgcacg
   102661 gcctccgctc agcggtgctt aaccatattc tggatacctt actggcgatc ccttcgctgc
   102721 tgctggcaat tatcgttgta gcgtttgccg gaccgagttt gtctcacgcc atgtttgccg
   102781 tctggctggc gctgctgccg cgtatggtac gttcgattta cagcatggtg catgacgaac
   102841 tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg aatattctct
   102901 ggtttgctgt gatgcctaac atcaccgccg ggctggtcac cgagatcacc cgcgcactgt
   102961 cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca caactcccct
   103021 cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc gccccgtgga
   103081 ctgtcatgct gccaggtgcg gcaattatga ttagcgtgct gttggttaac ctccttggcg
   103141 atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg atattcgtaa
   103201 cctgactatt gaatttaaaa ccggggatga gtgggttaaa gccgttgacc gggtaagtat
   103261 gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg gcaagagttt
   103321 gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg ctgaccgtat
   103381 gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca aactggttgg
   103441 ccacaacgtg tcgatgattt tccaggaacc gcagtcgtgt ctagatcctt cagaacgtgt
   103501 gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt ggtggcagcg
   103561 ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga ttaaagatca
   103621 caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc agaaagtgat
   103681 gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac cgacaaactc
   103741 aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca accaaaacag
   103801 caataccact attttgctta tcagccatga cttacaaatg cttagccaat gggcggataa
   103861 aattaacgta ctttactgcg gtcaaacggt ggaaaccgcg ccgagtaagg agttggtgac
   103921 gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact tcggcagcgc
   103981 gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct atcccactgc tggaacagtt
   104041 accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat gcattgtgac
   104101 gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc tgaacatgga
   104161 gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa acgttccgct
   104221 accggaccgg ctggtttcgt cgtcaaaccg tagaagcggt aaaacccttg agctttacgc
   104281 tacgtgaagg ccagacactg gcgattatcg gcgagaatgg ttcgggtaaa tccacgctgg
   104341 caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt gacgatcatc
   104401 cactgcattt tggcgattat tccttccgca gtcagcgtat tcgcatgatt tttcaggatc
   104461 cttcgacctc gttgaatccc cgtcaacgta tctcgcaaat tctggatttt ccactgcgcc
   104521 tgaacaccaa cctggaaccg gaacagcgtc gcaaacagat tattgaaacg atgcgtatgg
   104581 ttgggctgct accggatcac gtcagttact atccgcatat gttggccccc gggcaaaaac
   104641 agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt gccgatgaag
   104701 ccctcgcttc actggatatg tcgatgcgtt cgcagttgat taatctgatg ctggaattac
   104761 aggaaaaaca gggcatttcg tatatttatg tcacccagca tatcggaatg atgaagcaca
   104821 ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc agcaccgcgg
   104881 atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg cactttggcg
   104941 aagcattgac ggcggatgcg tggcgtaaag atcgctaatt ttctgaatcc tccttccaga
   105001 ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat ggataaaata
   105061 aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa gctctggcga
   105121 tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc ggtggggagc
   105181 tacattctcg attttgcttg ctgctcggcg cgtgtagtcg ttgagctgga tggtgggcag
   105241 catgatttgg cagttgccta tgattccagg cgcactaact ggcttgagtc gcagggctgg
   105301 accgtgctgc gtttctggaa taacgagatt gattgtaatg aggtggcggt gttggagatt
   105361 attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg ggccgaccga
   105421 gccgaatagc tgttgtggtg aaaatatgat gacagttctg gagagtattc ggcaaggtct
   105481 gaaccgtccc agccatcgcc ctgaaagggt tagggggctg tataagcctg tttgttgctg
   105541 gggtgacaat atttgcacaa tacggtcccc ctgcccctct gtggagagga ttagggtgag
   105601 ggaaaaagcg cccaccctgc ccgcgccctg ctccggtcgg acctggcaac tatagccacc
   105661 cacagccagg ttgattataa taaccgttta tctgttcgta ctgtttacta aaacgacgaa
   105721 tcgcctgatt ttcaggcaca acaagcatca acaataagga ttaaagctat gggttttctt
   105781 tccggtaagc gcattctggt aaccggtgtt gccagcaaac tgtccatcgc ctacggtatc
   105841 gctcaggcga tgcaccgcga aggagctgaa ctggcattca cctaccagaa cgacaaactg
   105901 aaaggccgcg tagaagaatt tgccgctcaa ttgggttctg acatcgttct gcagtgcgat
   105961 gttgccgaag atgccagcat cgacaccatg ttcgctgaac tggggaaagt ttggccgaaa
   106021 tttgacggtt tcgtacactc tattggtttt gcacctggcg atcagctgga tggtgactat
   106081 gttaacgccg ttacccgtga aggcttcaaa attgctcacg acatcagctc ctacagcttc
   106141 gttgcaatgg caaaagcttg ccgctccatg ctgaatccgg gttctgccct gctgaccctt
   106201 tcctaccttg gcgctgagcg cgctatcccg aactacaacg ttatgggtct ggcaaaagcg
   106261 tctctggaag cgaacgtgcg ctatatggcg aacgcgatgg gtccggaagg tgtgcgtgtt
   106321 aacgccatct ctgctggtcc gatccgtact ctggcggctt ccggtatcaa agacttccgc
   106381 aaaatgctgg ctcattgcga agccgttacc ccgattcgcc gtaccgttac tattgaagat
   106441 gtgggtaact ctgcggcatt cctgtgctcc gatctctctg ccggtatctc cggtgaagtg
   106501 gtccacgttg acggcggttt cagcatcgct gcaatgaacg aactcgaact gaaataattg
   106561 ttctgttggt aaagatgggc ggcgttctgc cgcccgttat ctctgttata cctttctgat
   106621 atttgttatc gccgatccgt ctttctcccc ttcccgcctt gcgtcaggat aacgatttcc
   106681 tttacgacca aggagcgccc atggaacaac gccacatcac cggcaaaagc cactggtatc
   106741 atgaaacgca atccagtact gcggagtatg acgttctgcc tctggtcccg gaagccgcaa
   106801 aggtcagcga tccctttctg ctcgacgtga tccttgatga agaaacgctg gcacccttcc
   106861 tttcatggct ggtccctgcg cgcgttcttg cagtagagtt gttccctgac cagcttaccg
   106921 tgacccgttc acagaccttc accgcttatg aacgcttgtc gacggccctg acggttgctc
   106981 aggtttgcgg cgtccagcgg ttatgtaact actattcggc gcgacttacg ccgctccccg
   107041 ggcctgattc ctccatggaa agtaatcatc ggttggcaca aatcacgcaa tatgcccgcc
   107101 aactggctag ctcgccttct attatcgaca accgatcgcg ccagcatctg aatgacgtcg
   107161 gtcttactgt ctgggactgt gtgatcatta accaaatcat tggttttatt ggctttcagg
   107221 cgcggacaat tgcgacattt caggcttatc tcgggcatcc ggtacgctgg ttacccgggc
   107281 tggagataca aaactacgcc gacgcgtcac agtttgctga tgaatcatta cgctggcgaa
   107341 gcagctatga ggtggaaaaa ctacctgaag agcacacaaa aagttcaact gcagaacttt
   107401 gccaactggc cgaaatactc tctctccacc ctatttcact ttcccttctc gaaaagttgt
   107461 taaacagcac acgggtcaat acacagccgg ataatcagct tgcggcgttg ttatgcgcgc
   107521 gtataaatgg cagtcctgct tgttttgccg cctgtatgga ttcatcaaat gaatataaaa
   107581 aaatcagccc ccttctgcgc aagggcgaaa atgaaattaa ccaatgggct gaccgtcatt
   107641 ctgttgagcg cgctaccgtt caggcgatac aatggctgac ccgagcaccc gatcgcttta
   107701 gcgccgccca gttcagccct ttactcgaac acgaaaaatc atcaacgcag attattaatc
   107761 tgctggtatg gagcgggctg tgtggctgga taaatcgctt aaaaatcgcg ttgggtgaga
   107821 catattaacc ttgccgcgtc agacagattc gcgtaaaact gtcagccgct ctaatggcca
   107881 ccaaaataga caattatgtt tcaggacaac ccgctgctag cgcagcttaa acagcaactg
   107941 cattcccaga cgccacgcgc tgaaggggtg gtaaaagcca cagaaaaagg ctttggcttc
   108001 ctggaagtcg acgcgcaaaa aagttatttc attccgccgc cgcagatgaa aaaagtcatg
   108061 catggcgacc gaattatcgc ggtgatccac agtgaaaaag aacgtgaatc cgcagagcca
   108121 gaagaactgg ttgaaccgtt cctgactcgt ttcgtgggta aagttcaggg caaaaatgac
   108181 cgtctggcca tcgttcctga tcatccactc ttaaaagacg ccattccttg ccgcgcagcc
   108241 cgtggcctga accacgagtt taaagaaggc gactgggcgg ttgccgaaat gcgccgtcat
   108301 ccgctgaaag gcgatcgttc tttctatgct gaactgacac aatacatcac ttttggtgac
   108361 gaccactttg taccgtggtg ggttacactt gcgcgccata atctggaaaa agaagcacca
   108421 gacggcgtcg ctaccgaaat gctcgatgaa ggtctggttc gtgaagatct gaccgcgctg
   108481 gattttgtca ccatcgacag tgccagcaca gaagatatgg atgacgccct tttcgctaag
   108541 gcgttgccgg atgacaaact tcagctgatt gtggcgattg ccgatccaac cgcgtggatt
   108601 gctgaaggca gcaagctgga caaagccgcg aaaattcgcg cattcaccaa ctatctgcct
   108661 ggcttcaaca tccctatgct tcctcgcgag ctttctgacg atctctgctc actgcgcgcc
   108721 aatgaagtcc gcccggtact ggcatgccgc atgacgctct ccgctgatgg caccattgaa
   108781 gataatatcg aattctttgc cgccaccatc gaatccaaag caaagctggt gtatgaccag
   108841 gtttctgact ggctggaaaa taccggtgac tggcagcctg aaagtgaagc aattgccgaa
   108901 caagtccgtt tgctagcgca aatttgccaa cgccgcggcg agtggcgtca taaccacgca
   108961 ctggtgttta aagatcgccc ggattaccgc tttattctcg gtgaaaaagg tgaagtgctg
   109021 gatatcgtcg ccgagcctcg tcgcattgcc aaccgtatcg tcgaagaagc gatgattgcc
   109081 gctaacattt gtgcggcccg cgtactgcgc gataagctcg gttttggcat ctataacgtg
   109141 catatgggct ttgatccggc gaatgccgac gcgctggcag cgttgctgaa aacgcacggt
   109201 ctgcatgtcg atgccgaaga agtgctcacg ctggacggtt tctgcaaact gcgtcgtgaa
   109261 ctggacgcgc aaccaactgg tttcctcgac agccgcattc gtcgcttcca gtcatttgct
   109321 gaaattagca ctgaacccgg tcctcacttt ggcctcggtc tggaagcata cgccacctgg
   109381 acttcgccga tccgtaaata tggcgacatg atcaaccacc gtctgctgaa agcggttatc
   109441 aaaggcgaaa ctgcgacgcg cccacaggat gaaatcactg tccaaatggc cgagcgtcgc
   109501 cgtctcaacc ggatggcaga acgtgatgtt ggtgactggt tatacgcacg cttcctgaaa
   109561 gacaaagccg ggaccgacac ccgtttcgca gcggaaattg tcgatatcag ccgtggcggc
   109621 atgcgtgttc gtttggttga taacggcgct atcgccttta ttccggcacc tttcttacac
   109681 gctgtgcgcg atgaactggt ttgcagccag gaaaacggca ctgtacaaat taaaggtgaa
   109741 acggtttaca aagtgactga cgttattgac gtcactattg ccgaagtccg catggaaacc
   109801 cgcagcatta ttgcgcgccc ggtcgcgtaa tctcctttca cggcccattc ctcatggatg
   109861 ggccgtttat ttccccgctc taccttcgtt atatccaggc agatttttta actgactttc
   109921 gtttgaaaac tggcgttttt ccaaaactgg attacaaata aatatataaa aaaaacaatg
   109981 cgtttagccg ccgttaattt catgtaagtc atgtagttcc ttttatttaa ccgcggactc
   110041 cgctgttaac cggaggatat gcatcatgaa aaccgttagg gagtccacaa cgttgtacaa
   110101 ctttctcgga tcgcacaatc catactggcg gttgacggaa agcagcgatg ttttgcgctt
   110161 ttctaccacc gaaaccacag aacctgatcg tacattgcag ttatctgccg aacaggctgc
   110221 tcgcatcagg gaaatgacgg tcatcacctc cagcctgatg atgagtctga ccgtcgatga
   110281 aagcgatctt tctgtgcatc tggtaggacg aaaaatcaat aaacgggaat gggctggcaa
   110341 cgcgtctgca tggcatgaca caccggcggt tgctcgtgat ttatcacacg ggctttcctt
   110401 tgctgagcag gtagtttctg aagcacattc cgcaatagtg attctcgaca gccgggggaa
   110461 tatccaacgc ttcaatcggt tatgtgaaga ttacacaggg ttgaaagaac acgacgtcat
   110521 tgggcaaagc gtgtttaaac tgtttatgag ccgtcgtgaa gctgcggcat ccaggcgcaa
   110581 taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc gaactgtgga taccaacatg
   110641 taaaggccag cggctgtttc tgtttcgcaa taaatttgtc cacagcggca gtggcaaaaa
   110701 cgagattttt ttaatctgtt ccggcaccga cattaccgaa gagcgccgcg ctcaggagcg
   110761 actgcgtatt ctggcaaata ccgacagtat caccggactg ccgaatcgta acgcaatgca
   110821 ggatttaatc gatcacgcta ttaatcatgc agataacaat aaagttgggg ttgtgtatct
   110881 tgatttggat aatttcaaaa aggtcaacga cgcctatggg catttgtttg gtgaccagtt
   110941 attacgcgac gtgtcattgg ctattttaag ctgtctcgaa catgaccagg tgttggcgcg
   111001 tccaggtggg gatgagtttc tggtactggc atccaacacc tcacaaagcg cgctggaagc
   111061 aatggcatca cgaattttga cccgcttacg gctccccttt cgcattggtt taattgaagt
   111121 ttataccagc tgttcagtag gtattgcact ctctcccgaa catggttcag acagcacggc
   111181 tattattcgt cacgccgaca cagcaatgta cacagcgaag gaaggcggac gaggacaatt
   111241 ttgcgttttt accccagaaa tgaatcaacg ggtatttgaa tatctctggc tggataccaa
   111301 cttgcgtaaa gcactggaaa acgatcagtt ggttattcac tatcaaccga aaatcacctg
   111361 gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg cagtcacctg aacgtgggtt
   111421 gattccaccg ttggacttca tttcctacgc cgaagagtca gggctaattg tgcctttagg
   111481 ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag tggcgggata aaggcataaa
   111541 cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc gatcaaacca ttttcaccgc
   111601 cctgaaacag gttctccagg aactcaattt tgaatactgc cctatagatg ttgaactgac
   111661 agagagttgt ctgattgaga atgatgaact ggcactgtct gttattcaac aatttagcca
   111721 actaggtgcg caagtgcatc tggatgattt tggtaccggc tactcttcac tttcgcaact
   111781 ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt tttgttcgag atattcacaa
   111841 acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct gtggcccagg cattgaatct
   111901 tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat gcttttttaa ccaagaacgg
   111961 gatcaatgag cggcaaggat ttttgtttgc caaaccgatg cccgccgtcg ccttcgaacg
   112021 ctggtataaa cgctatctga agcgcgcata agtagatgaa tatatatact tagcgctata
   112081 tcccgtcatt atatatcgta ttcctggatg ataattagcc gttgctgtta aacacttctc
   112141 ttttagcggc aatttttgca ttattcattt tccccctttt gctataagga tgtccttgat
   112201 gtctgaattt gatgcccagc gcgttgccga acgtattgat attgtgttag atatccttgt
   112261 ggcaggcgat taccactctg cgatccataa tctcgagata cttaaagcag aactcttgcg
   112321 ccaggttgct gaatctacgc cagacatccc gaaggcaccg tgggagattt aacaatcgtg
   112381 ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt atttttttat taagtattat
   112441 aacgttataa gaattacagc gatgaattcc cgacaacaaa ctattctaca gatggtcatt
   112501 gaccagggtc aggttagcgt aaccgatctg gcaaaagcca ctggagtttc tgaagttacc
   112561 attcgccagg atctcaacac actcgaaaaa ctgagttacc tccgccgtgc acatggcttt
   112621 gctgtttcgc ttgatagtga tgacgtcgaa acccgtatga tgagcaacta tacgctgaag
   112681 cgtgaactcg ccgagtttgc cgcgtcgctg gttcaaccgg gcgaaaccat ctttatcgaa
   112741 aatggcagca gcaatgccct actcgcccgg actctgggcg agcagaagaa aaatgtcact
   112801 atcatcacgg tcagcagcta catcgcgcat ttgctgaaag acgcgccttg tgaagttatt
   112861 ttgctcggtg gcgtgtatca gaaaaaaagc gaaagtatgg ttggcccttt gacacgccag
   112921 tgcatccaac aggtgcattt cagcaaagca tttattggta ttgatggctg gcaacctgaa
   112981 actggattta ccggtcgcga tatgatgcgt accgatgtgg tcaatgccgt gctggaaaaa
   113041 gagtgcgaag cgatagtcct gactgacagc tcgaaatttg gtgctgtaca ttcatactcc
   113101 atcggtcccg ttgagcgatt caatcgcgtg attaccgatt cgaaaatacg cgccagcgat
   113161 ctgatgcatc ttgagcacag taaactcacc gttcacgtcg ttgacattta accatttccc
   113221 ttctctacgg atgatttgca gtttggcaaa tcatccgctc taagatgatt cctggttgat
   113281 aattaagact atttacctgt tattaacact ctcaagatat aaaattatta tcagcgatat
   113341 aacaggaagt cattatcacc tgcgtgatat aaccctgcgc gcgagcagat ttcacggaat
   113401 aatttcacca ggcttattct tagctattat agttatagag agcttacttc cgtgaatcat
   113461 aaattcagga gagagtatta tgtttgtaac gagcaaaaaa atgaccgcgg ctgttctggc
   113521 aattactttg gcaatgtctc tgagtgcctg ttctaactgg tctaaacggg accgcaacac
   113581 cgcaatcggc gcgggtgcag gggcattagg cggtgcagta ctgaccgatg gcagtacgtt
   113641 gggtacatta ggtggtgcag ctgtcggtgg tgttattggt caccaggtcg gtaaataatc
   113701 gtaaataaat gtcgattatc gactcgcaat tataatacgc ttgattctgt tcagtacgaa
   113761 ataactgaat aatacgcaca gtaaaatatt cgaaagccac ggatatatcc gtggcttttt
   113821 atgttaaccg cctgcgagtt ttaccttcat ccctttcgct tcaagtagtg attttaataa
   113881 atcacgctta tcgccctgga tttcaataac tccatcttta actgctccgc cgcagccgca
   113941 ttttttcttc agttccgctg caagttttgt cagttcggca tcatcgagat cgacaccggt
   114001 aatcaggcaa acgcccttac ctttacgtcc actggtctga cgctgaatac gcaccacacc
   114061 gtcgccttta gggcgtacag gggccgcttt gggctcatca atacgtccgg tttccgttga
   114121 gtagaccaga cggctgttgg aatcactcat catgcactcc gctgtaaaga ggcgttgatc
   114181 gctttcagcg tctgcgctgg atctaccgat tgcgttaccg ggcgaccaat caccatataa
   114241 tcaacaccag ccgacaacgc ctgttctggc gtcataatgc ggcgctggtc accagcttca
   114301 ctcccctgcg gacgaatgcc cggcgtaacc agtttgaact cctgaccgaa tacctgttta
   114361 aagcgcacag cttcctgagc agaacacacc acaccatcaa ggccacattt ttgcgtcagt
   114421 gccgccagac gttctgcata atctgcaggt gacagtgtca tgccaagatc gaccaggtcg
   114481 ctggcttcca tgctggtcaa cactgtcaca gcaatcaaaa gcggtgcatc tttgccaaac
   114541 ggaaccagtg cctcacgcgc tgcggtcatc atacgcgccc caccagaggc atgaacattc
   114601 accatccaca cgcctaagtc agctgcagca gcgacagcgt gcgctgcagt gttggggata
   114661 tcgtggaatt tcaggtcaag aaagatatca aaaccacgct gttgaagttc gcgcacaaac
   114721 tgtggcccaa acaatgtaaa catctctttg ccgaccttca gacgacaatc gcgtgggtcg
   114781 atcttgtcga caaaggccag cgcgtcatca cgattatgat aatcaagggc aacaaccaca
   114841 ggagaattcg taacagcgcg ggaagaagat gaagcagtta acgtcatgac cagaccttct
   114901 tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg cgagcattct acccgtggaa
   114961 ataaaaaatt aacagttgcg atttctcccc tggcaggctt tttctgcgcc gtcgttgccg
   115021 gagcacaatg gaacattaat aattagtatg ttgtaactaa agtcggattt tttttaaaaa
   115081 ttacaggcca tcaagaccgc gaatcggttt aatggttgac caggcccgac aagacggaca
   115141 atgccagtag agggtgtatg cggtaaaacc acatttctgg caacgataac gaggcttact
   115201 acgtaccttc tcgccaacca tgtcacgcag caccatcagg ctctctttgg cacgcccttc
   115261 ttccgcttca tttaagtggt aatccattaa cttatggaac acacgcatgg tcggatgacg
   115321 ctgaagctgg cgcgtaatat agacctgtgc ggcctcacta ccgtcgcgcg cttcgatgat
   115381 atcagcaagc atcaattcag catcggcacc ggtgttctct tccaccgcac gctgcaggaa
   115441 ttctgcccat tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt
   115501 ttcgctgacc agttctctgt cctgggatat cacgcgttgc agactttcga cggctttggc
   115561 gtattctcct ttcgccataa acacgcgtcc catcattatg gatacgcggg cgctgttttt
   115621 atctgccgcc gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat
   115681 atgctgcagg gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc
   115741 cagcttcacc aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta
   115801 gatttgtagc aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt
   115861 gaacatgtct tccgcgcggt catataaccc ggcggccatg taatcacgcc ccagttgttg
   115921 aatcgccaac agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat
   115981 gcgaatagcg cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc
   116041 ttcaacggtg cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg
   116101 ttgattacta agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc
   116161 atcttgcttg ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac
   116221 aggcaaaagc agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg
   116281 cggcactaca gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg
   116341 ttcagcgcgc gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc
   116401 gatagcaaac cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg
   116461 cgctaacaga taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat
   116521 cacgaagatc gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat
   116581 gtggttagag cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt
   116641 cgcgccgtct gcgaaatggc atccacacgc attgttgatg atttaagggc taaattcgga
   116701 aaatcaacca gcgttaactt tcttgttctc gttgcgctat ttcgcgattt tcttccgcag
   116761 gtggtgttaa tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc
   116821 acgaaatcaa cgtagctact accagatcgc gtggccaatg catcccgagc agcaggcggc
   116881 ttcccatgac tcccgttgcc cagaccagca agatagcaat ggttaacgtt cgccgacgcg
   116941 gccacagcaa accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag
   117001 gaaaggcaaa ccccgtctct ttctgccagt gtgaacgcaa atattgtggg atatttttct
   117061 cttcagccaa ctgttctttc actagatttc cgcgttctgc tcgctttaaa gtgtagaact
   117121 catcaaccgg aatatgatgt gttttttcca gccagataac aaaaggtcgt ggttcctgga
   117181 ctttgtcttt gatccaggat ttaacgcctt gtcccacaag gattgcggcc gccagaatgg
   117241 caaataatac aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaata
   117301 aaatcaaatg tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg
   117361 cttttagtag ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta
   117421 ctggcatgac aagcaatagt gcagctccca ctgcggtacg tctggcaatc gaacgcatgg
   117481 cctctccttt ttgataagtc ccacaatcat aactgaaaac gccagttcca ggaaaaattg
   117541 acagatttgt gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt
   117601 tatggcaaaa taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac
   117661 gtgtggcaga agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag
   117721 aactggcaac cggacacgat catgtcgcgc tagtctatgg cgatatttcc gggcataccc
   117781 cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct
   117841 gcgattgtgg cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta
   117901 ttttgctgta tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt
   117961 acgcactgca ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg
   118021 ctgatgagcg cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag
   118081 tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata
   118141 ttgttgaacg cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata
   118201 ccaaagccga gaaaatgggc catttgctga acaaataacc ctcttgcatt gtgtaattca
   118261 tttgcttgcc ggaagcaaaa taaccggcaa gcaaatagtt gttacttcaa catattacga
   118321 atgacataat gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata
   118381 cgacaacggc agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc
   118441 gtcgcgccgg gttgtaggtt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc
   118501 cctaacgttt tacgcgttac gccttgcgga aattccagcg gcaggatgcc catgccaatt
   118561 aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat accaagcaga
   118621 cgcggacctt ttgccgccca gtcacgactg gagcctgatc catactcttt cccggcaatc
   118681 accgccagcg gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg
   118741 tcgctgtcag gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg
   118801 atgcgaatat tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg
   118861 taggagttaa agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg
   118921 tcgggcttaa tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt
   118981 gcgaggatcc gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca
   119041 aagaaaggcg ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt
   119101 gtgacgttaa ttcccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg
   119161 aacatttctg tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata
   119221 tctttcagat aaaccggatc gcctttgcga tcatggccga taggctcaga agccaggttg
   119281 atattcatat ttcccgccag cgcataggca accaccagcg gcggcgaggc cagccagtta
   119341 gttttaacca gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg
   119401 acggttaaat cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta
   119461 ccaatacagg tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt
   119521 gtcagtttcg cttttgccag ataatcagaa acgactttcg aacccggtgc cagcgacgct
   119581 ttgacccatg gttgccgctt gaggcccaga gttacggctt ttttcgccag caagcctgcg
   119641 gccatcagca cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca
   119701 gcgccatcag gtaactgata ctgatgtccg ttcataacat aatcgaccgg ctggcgatct
   119761 ttatgcgtgg cattcacttc cagttcgtta ctggcggcaa atgcttttgg tacatcgggc
   119821 agtgcaacgc gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata
   119881 tccagttcta acgtactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc
   119941 gctttggcat atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg
   120001 taatcgaggg ttacagcatc gattgggaag aagccacagg tggcaccata ttctggcgac
   120061 atattggcaa tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat
   120121 tcgacgaatt tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg
   120181 tctgtggcgg taataccttc acgtaatttt ccggtaagtt tgaagcccac tacgtccggg
   120241 ataagcatgg aaaccggctg gcctaacatt gcggcttctg cttcgatgcc accaacgccc
   120301 caccccagca cgccaaggcc gttgatcatg gtggtgtgcg agtcagtacc aacgagtgta
   120361 tccggataag caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat
   120421 tcgaggttaa cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac
   120481 gcctgctttc cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt
   120541 acgttttctt caaatgcctc atcatcacca aaacgatcga cagtcaccga gtggtcaatg
   120601 accaggtcga ccggtgagag cggattaacc tttgcagtat cgccgccgag gcgtttaacc
   120661 gcttcgcgca ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc
   120721 acccttgccg ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc
   120781 agcgcgtgga tatcctcttc ggtaaccgag ttaccatcct gccagcgcag caggttttcg
   120841 agcaaaactt tgagtgactt gggtagacgg gtgatatcgc ccagtgattt ggcagcaagc
   120901 ggcaggctgt agtagtggta agttttatct ttggcctgca acgcgtcctt actggcttct
   120961 cgtagggttg acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc
   121021 agggtgaata ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga
   121081 taaaagagga gaaaccttgt ggcaaagcaa atgaaaaccc cgccgcaacg gggtcaggga
   121141 tcagtgagta atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc
   121201 catccaggac caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta
   121261 ctttgtgttg agataactca acctgttatt gctgcggtgt attaaaagcc ctgtaattca
   121321 ttaaaaatgg gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac
   121381 agttctataa aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa
   121441 cattcccgtt tcgtttctgt ttcattgtca ctatgctggc ataatggtga cgactccagt
   121501 tgctcctgac cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc
   121561 agaaaagtgc agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcgc
   121621 ggcggggttc ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca
   121681 gcaagacgtt gaacgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt
   121741 tagtcaaaaa tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct
   121801 gaaatagata tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt
   121861 aatttaccct ataaatatga gggataaaaa tattgtgcac ggtatacaaa taagtaacaa
   121921 aagagaatat attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt
   121981 atttttccgg cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg
   122041 cgacagccgc atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat
   122101 acatataact gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga
   122161 agatatcgtg agcatcaacg cgcacaaggt ccggatcggc gaccggatcc accgccatgc
   122221 tggcaatgac ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg
   122281 ttgccgtgaa aacgatacgc ggcgttaacc ctgcgcgatt aaaggcagta tccagttctg
   122341 aacgtccggt aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg
   122401 taatggcttt tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt
   122461 ggtagcacgg taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga
   122521 aatcggcatt gcctttagag acggcatcag caatttgtgt cggcgagcct tggtgcatat
   122581 gcaaagaaac gcgaggataa cgctcaataa agcctttgat gacgtttggt aatgcgtagc
   122641 gtgcctgggt atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctctc
   122701 cggcaaccga ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt
   122761 cttgccctgc tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc
   122821 ctagctcgtc ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc
   122881 cttccgctgt tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt
   122941 gttgtaattt catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac
   123001 accaggcggc gtgactaata cgatttaata ataaaaaagg tgctaactat aac
//
LOCUS       DAZFZC010000002        99856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFZC010000002 DAZFZC010000000
VERSION     DAZFZC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 99856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.002"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1134
                     /gene="proV"
                     /locus_tag="ACHKXP_000117"
     CDS             <1..1134
                     /gene="proV"
                     /locus_tag="ACHKXP_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO7645201.1"
                     /translation="YIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGK
                     STMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLD
                     NTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINP
                     DILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNG
                     EVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSA
                     LKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLS
                     ELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1127..2191
                     /gene="proW"
                     /locus_tag="ACHKXP_000118"
     CDS             1127..2191
                     /gene="proW"
                     /locus_tag="ACHKXP_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO7645202.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2249..3241
                     /gene="proX"
                     /locus_tag="ACHKXP_000119"
     CDS             2249..3241
                     /gene="proX"
                     /locus_tag="ACHKXP_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO7645203.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3544..4728
                     /locus_tag="ACHKXP_000120"
     CDS             3544..4728
                     /locus_tag="ACHKXP_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7645204.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4852..5589
                     /gene="ygaZ"
                     /locus_tag="ACHKXP_000121"
     CDS             4852..5589
                     /gene="ygaZ"
                     /locus_tag="ACHKXP_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO7645205.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5579..5914
                     /gene="ygaH"
                     /locus_tag="ACHKXP_000122"
     CDS             5579..5914
                     /gene="ygaH"
                     /locus_tag="ACHKXP_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO7645206.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6005..6535
                     /gene="emrR"
                     /locus_tag="ACHKXP_000123"
     CDS             6005..6535
                     /gene="emrR"
                     /locus_tag="ACHKXP_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO7645207.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6662..7834
                     /gene="emrA"
                     /locus_tag="ACHKXP_000124"
     CDS             6662..7834
                     /gene="emrA"
                     /locus_tag="ACHKXP_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO7645208.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7851..9389
                     /gene="emrB"
                     /locus_tag="ACHKXP_000125"
     CDS             7851..9389
                     /gene="emrB"
                     /locus_tag="ACHKXP_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO7645209.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9453..9968)
                     /gene="luxS"
                     /locus_tag="ACHKXP_000126"
     CDS             complement(9453..9968)
                     /gene="luxS"
                     /locus_tag="ACHKXP_000126"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO7645210.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10118..11674)
                     /gene="gshA"
                     /locus_tag="ACHKXP_000127"
     CDS             complement(10118..11674)
                     /gene="gshA"
                     /locus_tag="ACHKXP_000127"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO7645211.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11747..12175)
                     /gene="yqaA"
                     /locus_tag="ACHKXP_000128"
     CDS             complement(11747..12175)
                     /gene="yqaA"
                     /locus_tag="ACHKXP_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO7645212.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12172..12738)
                     /gene="yqaB"
                     /locus_tag="ACHKXP_000129"
     CDS             complement(12172..12738)
                     /gene="yqaB"
                     /locus_tag="ACHKXP_000129"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO7645213.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13019..13095)
                     /locus_tag="ACHKXP_000130"
     tRNA            complement(13019..13095)
                     /locus_tag="ACHKXP_000130"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13059..13061),aa:Arg,seq:acg)
     gene            complement(13294..13370)
                     /locus_tag="ACHKXP_000131"
     tRNA            complement(13294..13370)
                     /locus_tag="ACHKXP_000131"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13334..13336),aa:Arg,seq:acg)
     gene            complement(13433..13509)
                     /locus_tag="ACHKXP_000132"
     tRNA            complement(13433..13509)
                     /locus_tag="ACHKXP_000132"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13473..13475),aa:Arg,seq:acg)
     gene            complement(13708..13784)
                     /locus_tag="ACHKXP_000133"
     tRNA            complement(13708..13784)
                     /locus_tag="ACHKXP_000133"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13748..13750),aa:Arg,seq:acg)
     gene            complement(13788..13880)
                     /locus_tag="ACHKXP_000134"
     tRNA            complement(13788..13880)
                     /locus_tag="ACHKXP_000134"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13844..13846),aa:Ser,seq:gct)
     gene            complement(14196..14381)
                     /gene="csrA"
                     /locus_tag="ACHKXP_000135"
     CDS             complement(14196..14381)
                     /gene="csrA"
                     /locus_tag="ACHKXP_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO7645214.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14616..17246)
                     /gene="alaS"
                     /locus_tag="ACHKXP_000136"
     CDS             complement(14616..17246)
                     /gene="alaS"
                     /locus_tag="ACHKXP_000136"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO7645215.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17374..17874)
                     /gene="recX"
                     /locus_tag="ACHKXP_000137"
     CDS             complement(17374..17874)
                     /gene="recX"
                     /locus_tag="ACHKXP_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO7645216.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQEI
                     WRNFAD"
     gene            complement(17943..19004)
                     /gene="recA"
                     /locus_tag="ACHKXP_000138"
     CDS             complement(17943..19004)
                     /gene="recA"
                     /locus_tag="ACHKXP_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO7645217.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19084..19581)
                     /gene="pncC"
                     /locus_tag="ACHKXP_000139"
     CDS             complement(19084..19581)
                     /gene="pncC"
                     /locus_tag="ACHKXP_000139"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO7645218.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19726..20811)
                     /gene="mltB"
                     /locus_tag="ACHKXP_000140"
     CDS             complement(19726..20811)
                     /gene="mltB"
                     /locus_tag="ACHKXP_000140"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO7645219.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21067..21630
                     /gene="srlA"
                     /locus_tag="ACHKXP_000141"
                     /gene_synonym="gutA"
     CDS             21067..21630
                     /gene="srlA"
                     /locus_tag="ACHKXP_000141"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO7645220.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21627..22586
                     /gene="srlE"
                     /locus_tag="ACHKXP_000142"
                     /gene_synonym="gutE"
     CDS             21627..22586
                     /gene="srlE"
                     /locus_tag="ACHKXP_000142"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO7645221.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSTVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22597..22968
                     /gene="srlB"
                     /locus_tag="ACHKXP_000143"
                     /gene_synonym="gutB"
     CDS             22597..22968
                     /gene="srlB"
                     /locus_tag="ACHKXP_000143"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO7645222.1"
                     /translation="MTVIYQTTITRIGASAIDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22972..23751
                     /gene="srlD"
                     /locus_tag="ACHKXP_000144"
     CDS             22972..23751
                     /gene="srlD"
                     /locus_tag="ACHKXP_000144"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO7645223.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            23856..24215
                     /gene="gutM"
                     /locus_tag="ACHKXP_000145"
     CDS             23856..24215
                     /gene="gutM"
                     /locus_tag="ACHKXP_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO7645224.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24282..25055
                     /gene="srlR"
                     /locus_tag="ACHKXP_000146"
                     /gene_synonym="gutR"
     CDS             24282..25055
                     /gene="srlR"
                     /locus_tag="ACHKXP_000146"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO7645225.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25048..26013
                     /gene="gutQ"
                     /locus_tag="ACHKXP_000147"
     CDS             25048..26013
                     /gene="gutQ"
                     /locus_tag="ACHKXP_000147"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO7645226.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26010..27524)
                     /gene="norR"
                     /locus_tag="ACHKXP_000148"
     CDS             complement(26010..27524)
                     /gene="norR"
                     /locus_tag="ACHKXP_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO7645227.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRCLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27711..29150
                     /gene="norV"
                     /locus_tag="ACHKXP_000149"
     CDS             27711..29150
                     /gene="norV"
                     /locus_tag="ACHKXP_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO7645228.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMAYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNSVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29147..30280
                     /gene="norW"
                     /locus_tag="ACHKXP_000150"
     CDS             29147..30280
                     /gene="norW"
                     /locus_tag="ACHKXP_000150"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO7645229.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DISHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQA
                     TLDRQRNIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNTDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(30408..32660)
                     /gene="hypF"
                     /locus_tag="ACHKXP_000151"
     CDS             complement(30408..32660)
                     /gene="hypF"
                     /locus_tag="ACHKXP_000151"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO7645230.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLYQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRFFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32813..33340)
                     /gene="hydN"
                     /locus_tag="ACHKXP_000152"
     CDS             complement(32813..33340)
                     /gene="hydN"
                     /locus_tag="ACHKXP_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO7645231.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33489..34499)
                     /gene="ascG"
                     /locus_tag="ACHKXP_000153"
     CDS             complement(33489..34499)
                     /gene="ascG"
                     /locus_tag="ACHKXP_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO7645232.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAP
                     SR"
     gene            34759..36216
                     /gene="ascF"
                     /locus_tag="ACHKXP_000154"
     CDS             34759..36216
                     /gene="ascF"
                     /locus_tag="ACHKXP_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO7645233.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKYQSVQPTVAKEVSLN"
     gene            36225..37649
                     /gene="ascB"
                     /locus_tag="ACHKXP_000155"
     CDS             36225..37649
                     /gene="ascB"
                     /locus_tag="ACHKXP_000155"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO7645234.1"
                     /translation="MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37808..38278)
                     /gene="hycI"
                     /locus_tag="ACHKXP_000156"
     CDS             complement(37808..38278)
                     /gene="hycI"
                     /locus_tag="ACHKXP_000156"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO7645235.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38271..38681)
                     /gene="hycH"
                     /locus_tag="ACHKXP_000157"
     CDS             complement(38271..38681)
                     /gene="hycH"
                     /locus_tag="ACHKXP_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO7645236.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(38678..39445)
                     /gene="hycG"
                     /locus_tag="ACHKXP_000158"
     CDS             complement(38678..39445)
                     /gene="hycG"
                     /locus_tag="ACHKXP_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO7645237.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(39445..39987)
                     /gene="hycF"
                     /locus_tag="ACHKXP_000159"
     CDS             complement(39445..39987)
                     /gene="hycF"
                     /locus_tag="ACHKXP_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO7645238.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(39997..41706)
                     /gene="hycE"
                     /locus_tag="ACHKXP_000160"
     CDS             complement(39997..41706)
                     /gene="hycE"
                     /locus_tag="ACHKXP_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO7645239.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(41724..42647)
                     /gene="hycD"
                     /locus_tag="ACHKXP_000161"
     CDS             complement(41724..42647)
                     /gene="hycD"
                     /locus_tag="ACHKXP_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO7645240.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRAARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(42650..44476)
                     /gene="hycC"
                     /locus_tag="ACHKXP_000162"
     CDS             complement(42650..44476)
                     /gene="hycC"
                     /locus_tag="ACHKXP_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO7645241.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSIWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44473..45084)
                     /gene="hycB"
                     /locus_tag="ACHKXP_000163"
     CDS             complement(44473..45084)
                     /gene="hycB"
                     /locus_tag="ACHKXP_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO7645242.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(45209..45670)
                     /gene="hycA"
                     /locus_tag="ACHKXP_000164"
     CDS             complement(45209..45670)
                     /gene="hycA"
                     /locus_tag="ACHKXP_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO7645243.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            45882..46232
                     /gene="hypA"
                     /locus_tag="ACHKXP_000165"
     CDS             45882..46232
                     /gene="hypA"
                     /locus_tag="ACHKXP_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7645244.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            46236..47108
                     /gene="hypB"
                     /locus_tag="ACHKXP_000166"
     CDS             46236..47108
                     /gene="hypB"
                     /locus_tag="ACHKXP_000166"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO7645245.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            47099..47371
                     /gene="hypC"
                     /locus_tag="ACHKXP_000167"
     CDS             47099..47371
                     /gene="hypC"
                     /locus_tag="ACHKXP_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO7645246.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47371..48492
                     /gene="hypD"
                     /locus_tag="ACHKXP_000168"
     CDS             47371..48492
                     /gene="hypD"
                     /locus_tag="ACHKXP_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO7645247.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            48489..49499
                     /gene="hypE"
                     /locus_tag="ACHKXP_000169"
     CDS             48489..49499
                     /gene="hypE"
                     /locus_tag="ACHKXP_000169"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO7645248.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRDAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            49573..51651
                     /gene="flhA"
                     /locus_tag="ACHKXP_000170"
     CDS             49573..51651
                     /gene="flhA"
                     /locus_tag="ACHKXP_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO7645249.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(51688..52041)
                     /gene="ygbA"
                     /locus_tag="ACHKXP_000171"
     CDS             complement(51688..52041)
                     /gene="ygbA"
                     /locus_tag="ACHKXP_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO7645250.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPHE"
     gene            complement(52073..52252)
                     /locus_tag="ACHKXP_000172"
     CDS             complement(52073..52252)
                     /locus_tag="ACHKXP_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645251.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            52328..54889
                     /gene="mutS"
                     /locus_tag="ACHKXP_000173"
     CDS             52328..54889
                     /gene="mutS"
                     /locus_tag="ACHKXP_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO7645252.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFIHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALKWIYRLKSLV"
     gene            54995..55651
                     /gene="pphB"
                     /locus_tag="ACHKXP_000174"
     CDS             54995..55651
                     /gene="pphB"
                     /locus_tag="ACHKXP_000174"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7645253.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEIINDNIKYAIAHADYPGDEYQFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(55702..56469)
                     /gene="ygbI"
                     /locus_tag="ACHKXP_000175"
     CDS             complement(55702..56469)
                     /gene="ygbI"
                     /locus_tag="ACHKXP_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO7645254.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            56665..57573
                     /gene="ltnD"
                     /locus_tag="ACHKXP_000176"
     CDS             56665..57573
                     /gene="ltnD"
                     /locus_tag="ACHKXP_000176"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO7645255.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            57570..58832
                     /gene="otnK"
                     /locus_tag="ACHKXP_000177"
     CDS             57570..58832
                     /gene="otnK"
                     /locus_tag="ACHKXP_000177"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO7645256.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQARKAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDDAFFSRAQREFLS"
     gene            58829..59467
                     /gene="ygbL"
                     /locus_tag="ACHKXP_000178"
     CDS             58829..59467
                     /gene="ygbL"
                     /locus_tag="ACHKXP_000178"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO7645257.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            59472..60248
                     /gene="ygbM"
                     /locus_tag="ACHKXP_000179"
     CDS             59472..60248
                     /gene="ygbM"
                     /locus_tag="ACHKXP_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO7645258.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            60337..61701
                     /gene="ygbN"
                     /locus_tag="ACHKXP_000180"
     CDS             60337..61701
                     /gene="ygbN"
                     /locus_tag="ACHKXP_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO7645259.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(61795..62787)
                     /gene="rpoS"
                     /locus_tag="ACHKXP_000181"
     CDS             complement(61795..62787)
                     /gene="rpoS"
                     /locus_tag="ACHKXP_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO7645260.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(62850..63944)
                     /gene="nlpD"
                     /locus_tag="ACHKXP_000182"
     CDS             complement(62850..63944)
                     /gene="nlpD"
                     /locus_tag="ACHKXP_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO7645261.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(64129..64755)
                     /gene="pcm"
                     /locus_tag="ACHKXP_000183"
     CDS             complement(64129..64755)
                     /gene="pcm"
                     /locus_tag="ACHKXP_000183"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO7645262.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(64749..65510)
                     /gene="surE"
                     /locus_tag="ACHKXP_000184"
     CDS             complement(64749..65510)
                     /gene="surE"
                     /locus_tag="ACHKXP_000184"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO7645263.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(65491..66540)
                     /gene="truD"
                     /locus_tag="ACHKXP_000185"
     CDS             complement(65491..66540)
                     /gene="truD"
                     /locus_tag="ACHKXP_000185"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO7645264.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(66537..67016)
                     /gene="ispF"
                     /locus_tag="ACHKXP_000186"
     CDS             complement(66537..67016)
                     /gene="ispF"
                     /locus_tag="ACHKXP_000186"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO7645265.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(67016..67726)
                     /gene="ispD"
                     /locus_tag="ACHKXP_000187"
     CDS             complement(67016..67726)
                     /gene="ispD"
                     /locus_tag="ACHKXP_000187"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO7645266.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(67745..68056)
                     /gene="ftsB"
                     /locus_tag="ACHKXP_000188"
     CDS             complement(67745..68056)
                     /gene="ftsB"
                     /locus_tag="ACHKXP_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO7645267.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(68250..68573)
                     /gene="ygbE"
                     /locus_tag="ACHKXP_000189"
     CDS             complement(68250..68573)
                     /gene="ygbE"
                     /locus_tag="ACHKXP_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO7645268.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(68623..69228)
                     /gene="cysC"
                     /locus_tag="ACHKXP_000190"
     CDS             complement(68623..69228)
                     /gene="cysC"
                     /locus_tag="ACHKXP_000190"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO7645269.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(69228..70655)
                     /gene="cysN"
                     /locus_tag="ACHKXP_000191"
     CDS             complement(69228..70655)
                     /gene="cysN"
                     /locus_tag="ACHKXP_000191"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO7645270.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(70657..71565)
                     /gene="cysD"
                     /locus_tag="ACHKXP_000192"
     CDS             complement(70657..71565)
                     /gene="cysD"
                     /locus_tag="ACHKXP_000192"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO7645271.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            71817..72854
                     /gene="iap"
                     /locus_tag="ACHKXP_000193"
     CDS             71817..72854
                     /gene="iap"
                     /locus_tag="ACHKXP_000193"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO7645272.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   72875..73818
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=72997..73025
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaactc"
     gene            complement(73924..74208)
                     /gene="cas2e"
                     /locus_tag="ACHKXP_000194"
     CDS             complement(73924..74208)
                     /gene="cas2e"
                     /locus_tag="ACHKXP_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO7645273.1"
                     /translation="MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIW
                     EQIAGLAEEGNVVMAWATNTESGFEFQTFGVNRRTPVDLDGLRLVSFLPV"
     gene            complement(74210..75127)
                     /gene="cas1e"
                     /locus_tag="ACHKXP_000195"
     CDS             complement(74210..75127)
                     /gene="cas1e"
                     /locus_tag="ACHKXP_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO7645274.1"
                     /translation="MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQ
                     AKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSCVRATYALLAKQYGVTW
                     NGRRYDPKDWEKGDTVNQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYD
                     IADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGE
                     IQPPAPPEDAQPVAIPLPVSLGDAGHRSS"
     gene            complement(75143..75742)
                     /gene="cas6e"
                     /locus_tag="ACHKXP_000196"
     CDS             complement(75143..75742)
                     /gene="cas6e"
                     /locus_tag="ACHKXP_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO7645275.1"
                     /translation="MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKR
                     NTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKR
                     LDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
                     VCFEGVITINDAPALIDLVQQGIGPAKSMGCGLLSLAPL"
     gene            complement(75729..76403)
                     /gene="cas5e"
                     /locus_tag="ACHKXP_000197"
     CDS             complement(75729..76403)
                     /gene="cas5e"
                     /locus_tag="ACHKXP_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO7645276.1"
                     /translation="MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACL
                     GIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHE
                     TIQTWREYLCDASFTVALWLTPQATMVMSELEKAVLKPRYTPYLGRRSCPLTQPLFLG
                     TCQASDPQKVLLNYEPVGGDIYSEESVDGHHLKFTVRDEPMITLPRQFASREWYVIKG
                     GMDVSQ"
     gene            complement(76406..77497)
                     /gene="cas7e"
                     /locus_tag="ACHKXP_000198"
     CDS             complement(76406..77497)
                     /gene="cas7e"
                     /locus_tag="ACHKXP_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO7645277.1"
                     /translation="MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSL
                     KRAMRKSDYYAQNIGESSLRTIHLAQLRDVLRQKLSERFDQKIIDKTLALLSGKSVDE
                     AEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
                     ALSGRMATSGMMSELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQE
                     FSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPA
                     DMVMVNFSDMPLSMANAFEKAVKANDGFLQPSLQAFNQYWDRVANGYGLNGAAAQFSL
                     SDVDPITGQVQQMPTLEQLKSWVRNNGEA"
     gene            complement(77510..77992)
                     /gene="casB"
                     /locus_tag="ACHKXP_000199"
                     /gene_synonym="cse2"
     CDS             complement(77510..77992)
                     /gene="casB"
                     /locus_tag="ACHKXP_000199"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO7645278.1"
                     /translation="MADEIDAMALYRAWQQLDNGACAQIRRVSEPDELRDIPAFYRLV
                     QPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKPEQTTGISLGRALANSGRINERRI
                     FQLIRADRTADMVQLRRLLAHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA
                     "
     gene            complement(77985..79493)
                     /gene="casA"
                     /locus_tag="ACHKXP_000200"
     CDS             complement(77985..79493)
                     /gene="casA"
                     /locus_tag="ACHKXP_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO7645279.1"
                     /translation="MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDME
                     LAALALLVCIGQIIDPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQ
                     TKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
                     GFKSGLRGGTPITTFVRGIDLRSTVLLNVLTIPRLQKQFPNESHTENQPTWVKPVKPN
                     ESVPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGL
                     WPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQF
                     RNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTAL
                     RKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADL
                     RDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG"
     gene            complement(79908..82574)
                     /gene="cas3"
                     /locus_tag="ACHKXP_000201"
     CDS             complement(79908..82574)
                     /gene="cas3"
                     /locus_tag="ACHKXP_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO7645280.1"
                     /translation="MEPFKYICHYWGKSSKSLTKGNDIHLLIYHCLDVAAVADCWWDQ
                     SVVLQNTFCRNEMLSKQRVKAWLLFFIALHDIGKFDIRFQYKSAESWLKLNPATPSLN
                     GPSTQMCRKFNHGAAGLYWFNQDSLSEQSLGDFFSFFDAAPHPYESWFPWVEAVTGHH
                     GFILHSQDQDKSRWEMPASLASYAAQDKQAREEWISVLEALFLTPAGLSINDIPPDCS
                     SLLAGFCSLADWLGSWTTTNTFLFNEDAPSDINALRTYFQDRQQDASRVLELSGLVSN
                     KRCYEGVHALLDNGYQPRQLQVLVDALPVAPGLTVIEAPTGSGKTETALAYAWKLIDQ
                     QIADSVIFALPTQATANAMLTRMEASASHLFSSPNLILAHGNSRFNHLFQSIKSRAIT
                     EQGQEEAWVQCCQWLSQSNKKVFLGQIGVCTIDQVLISVLPVKHRFIRGLGIGRSVLI
                     VDEVHAYDTYMNGLLEAVLKAQADVGGSVILLSATLPMKQKQKLLDTYGLHTDPVENN
                     SAYPLINWRGVNGAQRFDLLAHPEQLPPRFSIQPEPIYLADMLPDLTMLERMIAAANA
                     GAQVCLICNLVDVAQVCYQRLKELNNTQVDIDLFHARFTLNDRREKENRVISDFGKNG
                     ERNVGRILVATQVVEQSLDVDFDWLITQHCPADLLFQRLGRLHRHHRKYRPAGFEIPV
                     ATILLPDGEGYGRHEHIYSNVRVMWRTQQHIEELNGASLFFPDAYRQWLDSIYDDAEM
                     DEPEWVIKGMDKFESAECEKRFKARKVLQWAEEYSLQDNDETILAVTRDGEMSLPLLP
                     YVQTSSGKQLLDGQVYEDLSYEQQYEALALNRVNVPFTWKRSFSEVVDEDGLLWLEGK
                     QNQDGWFWQGNSIVITYTRDEGMTRVIPANPK"
     gene            complement(82933..83667)
                     /gene="cysH"
                     /locus_tag="ACHKXP_000202"
     CDS             complement(82933..83667)
                     /gene="cysH"
                     /locus_tag="ACHKXP_000202"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7645281.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(83741..85453)
                     /gene="cysI"
                     /locus_tag="ACHKXP_000203"
     CDS             complement(83741..85453)
                     /gene="cysI"
                     /locus_tag="ACHKXP_000203"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO7645282.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PHESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIAHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPARPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(85453..87252)
                     /gene="cysJ"
                     /locus_tag="ACHKXP_000204"
     CDS             complement(85453..87252)
                     /gene="cysJ"
                     /locus_tag="ACHKXP_000204"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO7645283.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            87571..87933
                     /gene="queD"
                     /locus_tag="ACHKXP_000205"
     CDS             87571..87933
                     /gene="queD"
                     /locus_tag="ACHKXP_000205"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO7645284.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            88011..89282
                     /gene="ygcN"
                     /locus_tag="ACHKXP_000206"
     CDS             88011..89282
                     /gene="ygcN"
                     /locus_tag="ACHKXP_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7645285.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            89273..89533
                     /gene="ygcO"
                     /locus_tag="ACHKXP_000207"
     CDS             89273..89533
                     /gene="ygcO"
                     /locus_tag="ACHKXP_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7645286.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            89550..90125
                     /gene="ygcP"
                     /locus_tag="ACHKXP_000208"
     CDS             89550..90125
                     /gene="ygcP"
                     /locus_tag="ACHKXP_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO7645287.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(90273..91133)
                     /gene="ygcQ"
                     /locus_tag="ACHKXP_000209"
     CDS             complement(90273..91133)
                     /gene="ygcQ"
                     /locus_tag="ACHKXP_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7645288.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(91130..91909)
                     /gene="ygcR"
                     /locus_tag="ACHKXP_000210"
     CDS             complement(91130..91909)
                     /gene="ygcR"
                     /locus_tag="ACHKXP_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO7645289.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(91887..93296)
                     /gene="ygcS"
                     /locus_tag="ACHKXP_000211"
     CDS             complement(91887..93296)
                     /gene="ygcS"
                     /locus_tag="ACHKXP_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7645290.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(93318..94772)
                     /gene="ygcU"
                     /locus_tag="ACHKXP_000212"
     CDS             complement(93318..94772)
                     /gene="ygcU"
                     /locus_tag="ACHKXP_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO7645291.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKVTGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(94842..95627)
                     /gene="ygcW"
                     /locus_tag="ACHKXP_000213"
     CDS             complement(94842..95627)
                     /gene="ygcW"
                     /locus_tag="ACHKXP_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7645292.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            95946..97223
                     /gene="yqcE"
                     /locus_tag="ACHKXP_000214"
     CDS             95946..97223
                     /gene="yqcE"
                     /locus_tag="ACHKXP_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO7645293.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQIAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLTLTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            97250..98728
                     /gene="ygcE"
                     /locus_tag="ACHKXP_000215"
     CDS             97250..98728
                     /gene="ygcE"
                     /locus_tag="ACHKXP_000215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO7645294.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDNMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT        23813 a        26706 c        25280 g        24057 t
ORIGIN      
        1 tatatcgaac aaggactttc aaaagaacaa attctggaaa aaactgggct atcgcttggc
       61 gtaaaagacg ccagtctggc cattgaagaa ggcgagatat ttgtcatcat gggattatcc
      121 ggctcgggta aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg
      181 caagtgctga ttgatggtgt ggatattgcc aaaatatccg acgccgaact ccgtgaggtg
      241 cgcagaaaaa agattgcgat ggtcttccag tcctttgcct taatgccgca tatgaccgtg
      301 ctggacaata ctgcgttcgg tatggaattg gccggaatta atgccgaaga acgccgggaa
      361 aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt atgcccacag ctacccggat
      421 gaactctctg gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc gattaatccg
      481 gatatattat taatggacga agccttctcg gcgctcgatc cattaattcg caccgagatg
      541 caggatgagc tggtaaaatt acaggcgaaa catcagcgca ccattgtctt tatttcccac
      601 gatcttgatg aagccatgcg tattggcgac cgaattgcca ttatgcaaaa tggtgaagtg
      661 gtacaggtcg gcacaccgga tgaaattctc aataatccgg cgaatgatta tgtccgtacc
      721 ttcttccgtg gcgttgatat tagtcaggta ttcagtgcga aagatattgc ccgccggaca
      781 ccgaatggct taattcgtaa aacccctggc ttcggcccac gttcggcact gaaattattg
      841 caggatgaag atcgcgaata tggctacgtt atcgaacgcg gtaataagtt tgtcggcgca
      901 gtctccatcg attcgcttaa aaccgcgtta acgcagcagc aaggtcttga tgcggcgctg
      961 attgatgcgc cgttagcagt cgatgcacaa acgcctctta gcgagttgct ctctcatgtc
     1021 ggacaggcac cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt cggcatcatt
     1081 tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg taaataatgg ctgatcaaaa
     1141 taatccgtgg gataccacgc cagcggcgga cagtgccgcg caatccgcag acgcctgggg
     1201 tacaccgacg actgcaccga ctgacggcgg tggtgctgac tggctgacca gtacgcctgc
     1261 gccaaacgtc gagcatttta atattctcga tccgttccat aaaacgctga tcccgctcga
     1321 cagttgggtc actgaaggga tcgactgggt cgttacccat ttccgtcccg tcttccaggg
     1381 cgtgcgcgtt ccggttgatt atatcctcaa cggtttccag caattgctgc tgggtatgcc
     1441 cgcaccggtg gcgattatcg ttttcgctct catcgcctgg cagatttccg gggtcggaat
     1501 gggtgtggcg acgctggttt cgctgattgc catcggcgca atcggtgcct ggtcgcaggc
     1561 aatggtgact ctggcgctgg tgttaaccgc cctgctgttc tgtatcgtca tcggtttgcc
     1621 gttggggata tggctggcga gaagtccgcg agcggcgaaa attattcgtc cactgcttga
     1681 tgccatgcag accacgccag cgtttgttta tctggtgcca atcgtcatgc tatttggtat
     1741 cggtaacgtg ccgggcgtgg tggtgacgat catctttgct ctgccgccga ttatccgtct
     1801 gaccattctg gggattaacc aggttccggc ggatctgatt gaagcctcgc gctcattcgg
     1861 tgccagcccg cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc cgaccattat
     1921 ggcgggcgtt aaccagacgc tgatgctggc cctttctatg gtggtcatcg cctcgatgat
     1981 tgccgtcggc gggttgggtc agatggtact tcgcggtatc ggtcgtctgg atatggggct
     2041 tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt atcctcgatc gtctgacgca
     2101 ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca ctggccctgt
     2161 tggtctgctg acccgcccat tcattaagta actctgcact tgcccggtga cgccgggcat
     2221 tatcaccctg ccaaaaaaag gaataacaat gcgacatagc gtactttttg cgacagcgtt
     2281 tgccacgctt atctctacac aaacttttgc tgccgatctg ccgggcaaag gcattactgt
     2341 taatccagtt cagagcacca tcactgaaga aaccttccag acgctgctgg tcagtcgtgc
     2401 gctggagaaa ttaggttata ccgtcaacaa acccagcgaa gtagattaca acgttggcta
     2461 cacctcgctt gcttccggcg atgcaacctt caccgccgtg aactggacgc cactgcatga
     2521 caacatgtac gaagctgccg gtggcgataa gaaattttat cgtgaagggg tatttgttaa
     2581 cggcgcggca cagggttacc tgatcgataa gaaaaccgcc gaccagtaca aaatcaccaa
     2641 catcgcacaa ctgaaagatc cgaagatcgc caaactgttc gataccaacg gcgacggaaa
     2701 agcggattta accggttgta accctggctg gggctgcgaa ggtgcgatca accaccagct
     2761 tgccgcgtat gaactgaccc ataccgtgac gcataatcag gggaactacg cggcgatgat
     2821 ggccgacacc atcagtcgct acaaagaggg caaaccggtg ttttactaca cctggacgcc
     2881 gtactgggtg agtaatgagc tgaagccagg gaaagatgtg gtctggttgc aggtgccgtt
     2941 ctccgcactg ccgggcgata aaaacgccga taccaaactg ccgaatggtg cgaattatgg
     3001 cttcccggtc agcaccatgc atatcgttgc caacaaagcc tgggccgaga aaaacccggc
     3061 agcagcgaaa ctgtttgcca ttatgcagtt gccagtggca gatattaacg cccagaacgc
     3121 cattatgcat gacggcaaag cctcagaagg cgatattcag ggccatgttg atggctggat
     3181 caaagcccac cagcagcagt tcgatggctg ggtgaatgag gcgctggcag cgcagaagta
     3241 atttttattc gggcggataa ggcgttttta cctcatccgc cgctctgtac acaatgcctg
     3301 atgcgacgct ggagcgtctt atcatgccta caaaatacac tcattccgta tggcggataa
     3361 ggcgttttcg ccgcatccgt cgttctgtgc acaatgcctg atgcgacgct ggcgcgtctt
     3421 atcatgccta caaaccacat cgcacatttc aacaagctat ctttcatccc atattcatca
     3481 acatccgcta ttattgattt ccagttgatt tccagcttaa tcatcacctg atgaacaaaa
     3541 ataatgacta aacctaatca tgagcttagc ccggcgctga tcgtgctgat gtctatcgcc
     3601 accggtctgg cggtcgccag taactattac gctcagccat tgctcgacac catcgcgcgt
     3661 aacttttccc tttccgccag ttcggcaggc tttattgtta ccgccgcgca attgggctat
     3721 gcagcaggtc tgctgtttct tgttcccctc ggtgatatgt ttgaacgccg ccgcctgatt
     3781 gtctcgatga ccttactggc ggcaggcggc atgttaatca ctgccagcag tcagtcgctg
     3841 gcgatgatga tcctcggtac ggcattaacc ggtttattct ctgtcgtggc acaaattctg
     3901 gttccgctgg cagcgacgct ggcttcaccg gacaaacgcg gcaaagtggt tggcactatt
     3961 atgagcggtc tgctgttggg gatcttgctg gcacggacag ttgccggatt gctggcgaat
     4021 ctcggcggct ggcgcaccgt cttttgggtt gcttcggtgt taatggcact gatggcgctg
     4081 gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta cccacagttg
     4141 ttgggttccg ttttcagtat gtttatcagc gataaaattc tgcgtacccg cgcgttgctg
     4201 ggctgcctga cctttgccaa tttcagcatt ctctggacct caatggcctt tttgcttgcc
     4261 gctccacctt ttaactacag cgatggtgta attggtctgt ttggacttgc gggagctgcc
     4321 ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgata agggcaaatc gcaccacacc
     4381 acaactttcg gtctgctgct gctattactt tcatggctgg cgatctggtt tggtcacact
     4441 tccgtactgg cgttgattat cggaatcctg gtgctggatc tcaccgtgca gggcgtgcat
     4501 atcactaacc agacggtaat ttatcggata catcctgatg cgcgcaatcg cctgaccgca
     4561 ggttacatga ctagctactt tattggcggt gccgccggtt cgctaatttc agcctcagcc
     4621 tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc cctggttaac
     4681 ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc aatcagcaac
     4741 cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg ataaggttaa
     4801 gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc tatggaaagc
     4861 cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa agacagttta
     4921 ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc gacccgtctg
     4981 ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg cgcgagccag
     5041 ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt ggattgctgc actgaccgtc
     5101 atggcaatgg atgttcgcca tgtgttgtat ggcccgtcac tgcgtagccg tattattcag
     5161 cgtctgcaaa aatcgaaaac cgccctgtgg gcgtttggcc tgacggatga ggtttttgcc
     5221 gccgcaaccg caaaactggt acgcaataat cgccgctgga gcgagaactg gatgatcggc
     5281 attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc attctccggc
     5341 agcggcttgc tgcaaggtta tcccgccgtt gaagctgcat taggttttat gcttccggca
     5401 ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg cgttaccgca
     5461 gcgttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc cattctggca
     5521 ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg agcgcccgat
     5581 gagctatgag gttctgctgc ttgggttact ggttggcgtg gcgaattatt gcttccgcta
     5641 tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg cggtaggtat
     5701 tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct ctaccgcacc
     5761 agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg cggtactggg
     5821 tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg cgctggccta
     5881 tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt ttctaaaaca
     5941 atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat gaggtcatac
     6001 ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg ccagccgcca
     6061 cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca tgcaaagcaa
     6121 gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga cgttgtttat
     6181 ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg aattaagttg
     6241 tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg aaaaacgcgg
     6301 ttggatcgaa cgtcgtgaaa gcgataacga tcgccgctgc ctgcatctgc aattaacgga
     6361 aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc tgcatcaact
     6421 ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc gcaaattgct
     6481 ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga gctaacgcgt
     6541 catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca tcgcaacatg
     6601 ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg tggagaacaa
     6661 tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga gcggcaaacg
     6721 taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag cgatagggat
     6781 ttattggttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg tggcagggaa
     6841 tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg ccgataacac
     6901 cgattttgta aaagaaggcg atgttctggt cactctcgac ccgacagatg ctcgccaggc
     6961 gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc agctgatgat
     7021 taacagcaaa cagttgcagg cgaatattga ggtgcaaaaa atcgccctcg cgaaagcaca
     7081 aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc gcgaagagct
     7141 gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg cgattcaaca
     7201 atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc cagccgtgca
     7261 acaggctgcc accgaagtac gtaacgcctg gctggcgctg gagcgtactc gtattgtcag
     7321 tccgatgacc ggttatgtct cccgccgcgc ggtacagcct ggggcgcaaa ttagcccaac
     7381 gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca actttaaaga
     7441 gacgcagatt gccaatatgc gtatcggtca gccggtcact atcaccacgg atatttacgg
     7501 cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag gtagcgcgtt
     7561 ctcactgctt ccagcgcaaa atgcgaccgg taactggatc aaagtcgttc agcgtctgcc
     7621 tgtgcgtatc gaactggacc agaaacagct ggagcaatat ccgctgcgta tcggtttgtc
     7681 cacgctggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa ataaagtacg
     7741 ttccactccg gtagcggtaa gcaccgcgcg tgaaatcagc ctggcacctg tcaataaact
     7801 gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg atgcaacagc
     7861 aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca ctggcgacat
     7921 tcatgcaggt gctggactcc accattgcta acgtggcgat ccccactatc gccgggaatc
     7981 tgggctcatc gctcagccag ggaacgtggg taatcacttc tttcggggtg gcgaatgcca
     8041 tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa ctgttccttt
     8101 ggtccaccat cgcctttgct attgcgtcgt gggcgtgtgg tgtctccagc agcctgaata
     8161 tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc ccgctttcgc
     8221 aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg gcgttgtggt
     8281 cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat atcagcgata
     8341 attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg gtggtgttga
     8401 tgacactgca aactctgcgc ggacgtgaaa cccgcaccga acggcggcgg attgatgccg
     8461 tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc gaccgcggta
     8521 aagagctgga ctggttttca tcacaggaaa ttatcatcct taccgtggtg gcggtggtgg
     8581 ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc gatctgtcgt
     8641 tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg tatatgctct
     8701 acttcggcgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt tacacggcaa
     8761 cgtgggcagg tctggcctct gcgccggtag ggattattcc ggtgatcctg tcgccgatta
     8821 tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc tttattatgt
     8881 atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat tttggcgcgt
     8941 cggcctggcc gcagtttatc caggggtttg cggtggcctg cttctttatg ccgctgacca
     9001 ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc ctctctaact
     9061 ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg tggaccaacc
     9121 gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac ccgaatgccc
     9181 aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca tcaggctgga
     9241 ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc ttctggatgt
     9301 cagccgggat attcctcgtc ctgctggggc tggtgtggtt tgctaaaccg ccatttggcg
     9361 caggtggcgg cggaggcggt gcgcactaag tacaactaag ccagttcatt tgaactggct
     9421 tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg caacttctct
     9481 ttcggcagtg ccagttcttc gttgctgttg atgcgtacat cacgttccag aatgctacgc
     9541 gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg gtagacgttc
     9601 agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc cgctttccag
     9661 gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa accggtgcgg
     9721 cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg gttacgcata
     9781 aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac ttctttgttc
     9841 ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt gttcattgtt
     9901 ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa gctatctaac
     9961 aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct ttcttcccag
    10021 gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat tcagagatga
    10081 aattttggcc actcacgagt ggcctttttc ttttctgtca ggcgtgtttt tccagccaca
    10141 ccgcaaacgg ttcggtatca gcggcttcca tttcctgctg acggcgttca gacgcctcgc
    10201 gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc agcagattac
    10261 ggtaggcttc tgcaaatgct ttgcctgttc cgccaatacc agtatcaatc atagacctta
    10321 agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt tcatcacaca
    10381 ctttctgata cgcttcgccg ccgttaatac tatccagcgt ttgcgcgacg cgtttcagat
    10441 cgcggaacag atctttaccc acctgcggta acgggaactg tgcggtttcg cagccgatac
    10501 ccagcgtcag acccggtttg cgaccttcga ggatcacccg gttccagtta acgcgtgtac
    10561 aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag accataaaca
    10621 ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg ttgatgtcca
    10681 gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa ggcgactcgc
    10741 cgctgcgggt aacgcgtttt ggacgaatcg gcgcgtacag ttcgttttca atctgcaaca
    10801 cgttgctgtt gatttgcagc ctcttaccgt ctttctcaat accaatcttc gcgtactctt
    10861 ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga tcgttgaagg
    10921 taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa cgaagagagg
    10981 tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc gacgttggtt
    11041 ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa ggaatgaccc
    11101 aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt ttctctttgg
    11161 catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc aaagagaaat
    11221 tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc agcccttcac
    11281 gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt tcgatgtcct
    11341 gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg cccatattgc
    11401 gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata tcaccatcca
    11461 ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat ttgtgcgtca
    11521 gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca gcattaacac
    11581 gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga tgtttttcca
    11641 gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg tcaaaatcgt
    11701 tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag tgccaccaca
    11761 tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta ccaaggcaca
    11821 aaaaaaagat taccggtccc cacgagatgc gcatccatcc cgctaacaga cacagtaaat
    11881 cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt ttcagccagc
    11941 cggtagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc ccaaggataa
    12001 cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa acccagggat
    12061 gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg ggtaatagtg
    12121 tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca ctcacagcaa
    12181 gcgaacatcc acggcgtcca tgcctgctgc acgggccgcc tgaataccga aatcggcatc
    12241 ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca acaaaaatgt
    12301 gtctggcgcg ggtttatggt gtttgacgtg atcggcagcg acgacggcgt caaaataatg
    12361 gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc ccgttcctac
    12421 agccattggg cgacgaccat gccaactttt caccacatca acaagaggaa gcggttcgac
    12481 gctatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg catgcgggtc
    12541 gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg gcgatccatt
    12601 aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata cttcgcgcca
    12661 cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa aaattaaacc
    12721 tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta ctttagcgta
    12781 attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg gataaaggag
    12841 ttatttagaa atgagatatt tttgaaggaa attttttgga gaagatggtg catccgggag
    12901 gatgactcac tgacgttcgc ccttcgggcc gttgctggcg caacgttatc cttcctggtg
    12961 cctgcggtta ataccgctat ctcttcgata ccttcattgc tgaaggttac ggagaatatg
    13021 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    13081 ctgagctacg gatgcatcgg gaaaacttat tctactgctg atattgatac cgctactaaa
    13141 gccatatcaa ttaagagatg gtgcatccgg gaggattact cgccttgcgg ctcgcccttc
    13201 gggccgttgc taaagcaacg ttatcatccc tagtgcctgc ggttaattcc gctacctctt
    13261 cgataccttc attgctgaag atttcggaga atatggtgca tccgggagga ttcgaacctc
    13321 cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaat
    13381 ttattttact gctgatattg ataccgctac taaagccata tcaagtaaga gatggtgcat
    13441 ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat ccagctgagc
    13501 tacggatgca tcgggaaact tactttactg cagatttttt gataccgcta ctaaagccgt
    13561 atcaagtaag agatggtgca tccgggagga ttactcgcct tgcggctcgc ccttcgggcc
    13621 gttgctaaag caacgttatc ctccctggtg cctgcggtta ttaccgccac ctcttccata
    13681 ccttcatcgc tgaaagtacg tagaatatgg tgcatccggg aggattcgaa cctccgaccg
    13741 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
    13801 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
    13861 agcctctcgg ccacctcacc acacgtctct tacgagtgct tcgaagaact tgtttattgc
    13921 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
    13981 ccacactttt atcgtttgca cacttcacgc tcaattagtc tgtaaaaacg tcaaaaagag
    14041 tgttttatct acagaaaaac ggaggtctgg cagatagcag taatgcaaaa aaatggagac
    14101 ttaagttgaa tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg
    14161 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
    14221 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
    14281 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
    14341 atcatgaggg tttcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
    14401 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
    14461 caatgacaaa cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt
    14521 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
    14581 gtgaagcgag tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag
    14641 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
    14701 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
    14761 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
    14821 tcggctaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
    14881 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
    14941 ttaacatcaa ttgccttact ggaaagattt gcactctcct gtgcggcagc ttgttcttta
    15001 agctgttgta actctttttc cagctgacgc gtacgttcca gtactgaacg cactttatca
    15061 gccagattat tgctatcgcc tttcagcaga tgtgcgactt cgcttaagcg atcgctgtct
    15121 gcatgaacgg tggcgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca
    15181 gcagtacccg attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga
    15241 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac gcgctcatca
    15301 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
    15361 ttggtttcga taggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga
    15421 atctcttccg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac tttgtcgtta
    15481 accagtgaac ctttctgcga tacatgagta cccagaacct ggcgcagcgc agcgtgcatc
    15541 aggtgcgttg cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc
    15601 tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
    15661 tggccgtatt tctgcgtatc ttccaccgca aaggagaagt tagcgccttt cagttcgcct
    15721 ttatcgccaa cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca
    15781 gcttcctggc ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcagtcact
    15841 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactgtc aacacggatc
    15901 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc
    15961 attgcagctt caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
    16021 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagcagtttc accatccagc
    16081 gtatcaccag aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta
    16141 cgagcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
    16201 aggtcttcac ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag
    16261 gtttctttcg cgccgagcat attaccgtga cgcactgcgc gacgaatgat acgacgcagt
    16321 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
    16381 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggttgc gccagtgact
    16441 ttcgctaccg cctggatcag cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
    16501 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc
    16561 ggttccatcg tgccatcggc ctggcggttg aactgcatga agacgatgtt ccagatctca
    16621 atgtagcggt cgccgtcttc ttccgggctt cccggagggc ccccccaaat gtggtcgccg
    16681 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
    16741 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
    16801 atccctactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
    16861 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
    16921 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
    16981 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
    17041 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
    17101 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
    17161 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
    17221 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
    17281 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
    17341 tttcttgact gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatctc
    17401 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
    17461 tgaaaagaca gttggcaaag gttcgccata ttttcgcgtc gcctgatcgc gcgccagtgc
    17521 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
    17581 ctgattcagt tcctggcgaa tacgcgcagg tccataacct ttgcggctac ggctggcgat
    17641 aaagcgcgca acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
    17701 gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg
    17761 tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
    17821 cgcacgatcc aacaggcgag catatgccgg gcgacgggat gttgattctg tcatggcata
    17881 tccttacaac ttaaaaaagc aaaagggccg cagatgcgac ccttgtgtat caaacaagac
    17941 gattaaaaat cttcgttagt ttctgctacg ccttcgctat catctacaga gaaatccggc
    18001 gttgagttcg ggttgctcag cagcaactca cgtactttct tctcgatctc tttcgcggtt
    18061 tccgggttat ctttcagcca ggcagtcgca ttcgctttac cctgaccgat cttctcacct
    18121 ttgtagctgt accacgcgcc tgctttctcg atcagcttct cttttacgcc caggtcaacc
    18181 agttcgccgt agaagttgat accttcgccg tagaggatct ggaattcagc ctgtttaaac
    18241 ggcgcagcga ttttgttctt caccactttc acgcgggttt cgctacccac cacgttttcg
    18301 ccctctttca ccgcgccgat acgacggatg tcgagacgaa cagaggcgta gaatttcagc
    18361 gcgttaccac cggtagtggt ttccgggtta ccgaacatca caccaatttt catacggatc
    18421 tggttgatga agatcagcag cgtgttggac tgcttcaggt tacccgccag cttacgcatc
    18481 gcctggctca tcatacgtgc cgcaaggccc atgtgagagt cgccgatttc gccttcgatt
    18541 tccgctttcg gcgtcagtgc cgccacggag tcaacgacga taacgtctac tgcgccagaa
    18601 cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg tgtccggctg ggagcacagc
    18661 aggttgtcga tatcgacgcc cagtttacgt gcgtagattg ggtccagcgc gtgttcagca
    18721 tcgataaacg cacaggtttt accttcacgc tgcgctgcgg cgatcacctg cagcgtcagc
    18781 gtggttttac cggaagattc cggtccgtag atttcgacga tacggcccat cggcagacca
    18841 cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag agatggtttc cacatccatg
    18901 gaacggtctt cacccaggcg catgatggag cctttaccaa attgtttctc aatctggccc
    18961 agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag ccatttttac tcctgtcatg
    19021 ccgggtaata ccggatagtc aatatgttct gttgaagcaa ttatactgta tgctcataca
    19081 gtatcaagtg ttttgtagaa attgttgcca caaggtctgc aatgcatacg cagtagcctg
    19141 acgacgcacc gcatcacggt cgccgctgaa gcattcccgc cgggtaatgc cttcaccgcg
    19201 ggcagtggca aaagcaaacc agacggtgcc gacaggcttc tcttcactgc cgccatccgg
    19261 cccggcgata ccactaatag acacggcata atcagcacga gccgctttca gtgcgcctat
    19321 cgccatttcc accacgacgg gttcactcac cgcgccatgc tgcgccagcg tctcttcgcg
    19381 tacgccgatc atctgcgctt tggcttcgtt actgtaggtg acaaatccgc gttcaaacca
    19441 ggcggagcta ccggcaatat cggtaatcac tttcgctacc caaccaccgg tacaagactc
    19501 ggcagttgtt acggttgcgc cacgggcttt cagcgcctgc ccaacctgtt cacttaactg
    19561 catcagttca ctgtcagtca tcacaacctc tgtcagtcaa aaatttatgc cggacaagat
    19621 agcactttcg tcaggaagat ggtgttggcg ttgcaatttt acgaggagga ttcagaaaaa
    19681 agctgattag ccagagggaa gctcacgccc ccctcttgta aatagctact gtactcgcgc
    19741 cagcgccacg gcttgtccta actgccagac cgccattgcg taatgggtgc tgtggttgta
    19801 acgggtgatg gtgtagaagt tcggcagacc gtaccagtac tggtagccgg tgccaacatc
    19861 cagacgcagc aggctggctt gttgatggtt gcccagcggc tgctgtggcg ttaaacctgc
    19921 ggcggcaagc tgcgaaatgc tgtacttagt tttgaagcca tttggcaagc ctggagcctg
    19981 accgtttgcc attaccgcga cctgatcgcc tttcacccag ccgtgcgctt tgaaatagtt
    20041 cgccacgcta ccgatcgcat caaccggatc ccacaggttg atatgcccgt cgccgctgaa
    20101 atctaccgca tattgtttgt aagacgacgg cataaactgt ccgtagccca tcgccccggc
    20161 aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg cgcgccatca acaggaaggt
    20221 ttccagctcg ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag
    20281 cgcatcgagg atgcgagttt tccccatcac gcgcccccag cgggtttcaa cgccgataat
    20341 cccgacgata atttccggcg gtactccata cacctgccac gcgcgattca acgcatcttc
    20401 atactgattc cagaaaacca caccgttctg cacgttgtcc ggcgtaataa attttttgcg
    20461 ataacggagc catgcgccgt tcggacctga tggcggtttc accgatgtgg ttggtgcctg
    20521 gttatccatc agccgcagta ccgaatccag acgcttcgcc tgggagagaa tttcctgcaa
    20581 ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg atgaactgct gggcattcgg
    20641 gttattagcg aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc
    20701 gccagacggc gttccggtgg tcgtatcagt ctcagtaggt tttggcttgc tgctacaggc
    20761 ggcaagcaac acaaaaaggg gaagcaatgt tacataacga cgcttgaaca tgaggggtcc
    20821 atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc acgccccgca caaggaagcg
    20881 gtagtcactg cccgatacgg actttacata actcaactca ttcccctcgc tatcctttta
    20941 ttcaaacttt caaattaaaa tatttatctt tcattttgcg atcaaaataa cacttttaaa
    21001 tctttcaatc tgattagatt aggttgccgt ttggtaataa aacaataaat cctgaaggag
    21061 agaacaatga tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag
    21121 ggcggagagg tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgctg
    21181 gttatcatga acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa
    21241 cgttgcgccg gtaaccctgt ttcccgttac ctactgttac cgtgcattgg cacgtttgtc
    21301 ttttgcaatc cgatgaccct aagcctgggt cgctttatgc cggaaaagta caaacccagc
    21361 tactacgcgg cggcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac
    21421 cctggcgaac tgtttgttta tcttggcatt gccagcggtc tgacaacgct gaacctgcca
    21481 cttggcccac tggcggtgag ttatctgctg gttggtctgg tcaccaattt cttccgcggc
    21541 tgggtgaccg atctgaccac cgccattttt gagaaaaaga tgggcattca acttgaacaa
    21601 aaagttcacc tggcaggagc aacatcatga cgcatattcg gatcgaaaaa ggaacgggtg
    21661 gctggggcgg cccgcttgag ctgaaagcca cgccgggaaa aaaaatcgtc tatatcaccg
    21721 ccggtacccg gcctgcgatt gttgacaaac tggcacagct tactggctgg caggctattg
    21781 acggatttaa agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta atcgactgtg
    21841 gcggcacatt acgctgcggc atctatccga aacgacgtat tcccaccatt aatatccact
    21901 cgacgggcaa gtccggtccg ctggcgcagt acattgtgga agatatttat gtctctggcg
    21961 taaaagaaga aaacatcact gtagtaggtg atgcgacacc acaaccctct tccgtgggcc
    22021 gtgactatga caccagtaag aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg
    22081 gaatgggcat ggggtccacc gttgcggtgc tgtttcaatc tggtcgtgac accatcgaca
    22141 ctgtattaaa aaccattctg ccgtttatgg cattcgtctc ggcgctcatt ggcatcatta
    22201 tggcttctgg ccttggtgac tggattgccc acggtcttgc tccgctggcg agccatccac
    22261 tgggtctggt catgctggcg ctcatctgtt ccttcccact gctttcacct ttcctcggcc
    22321 caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt ggtctcggca
    22381 atattccgcc gcatctggct ttaccggcac tgtttgccat caacgcgcag gcggcctgcg
    22441 acttcatccc ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt cgcgtcggtg
    22501 tcccttctgt actggtgagc cgctttttaa ccggcgcgcc gactgtactg atcgcctggt
    22561 ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta tcagaccacc
    22621 atcacccgta tcggcgcgag tgccattgac gccctcagtg accagatgct catcaccttt
    22681 cgtgaaggcg cgcctgcgga cctcgaagag tattgcttca ttcattgcca cggcgagttg
    22741 aaaggtgcac tccatcccgg tttgcaattt tcactcgggc agcatcgcta tccggtgacc
    22801 gctgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc
    22861 gatggtttaa acgaagcgga atttccgggc actgtccatg tggcaggccc tgtccccgac
    22921 gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa aatgaatcag
    22981 gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct
    23041 gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc aaatgtggca
    23101 caagaaatta acgccgaata tggtgaaagt atggcgtacg gttttggtgc tgacgccact
    23161 agcgagcaaa gcgttctggc gctctctcgt ggggtagatg aaatctttgg tcgcgtggat
    23221 ttgctggtct acagcgccgg aatagcaaaa gcagccttta tcagcgactt ccagctcggc
    23281 gattttgacc gttcgctaca ggtgaatctg gtgggttatt tcctgtgtgc gcgtgaattt
    23341 tcgcgtttga tgatccgcga cgggattcag gggcgcatta ttcagatcaa ctcgaaatcc
    23401 ggcaaagtgg gcagcaaaca caactctggc tacagcgcag cgaaatttgg tggcgtcggg
    23461 ctgactcaat cactggcgct ggatctggcg gagtacggca ttacggtgca ttcactgatg
    23521 ctcggtaacc tgctgaaatc gccgatgttc cagtcactgt tgccacaata cgcgaccaag
    23581 ctgggtatca aaccggatca agtcgagcag tattacatcg acaaagtacc gctcaaacgc
    23641 ggctgcgatt atcaagatgt gctgaatatg ctgctgttct acgccagtcc taaggcgtcg
    23701 tactgcaccg gacagtcgat caatgtcacc ggcggtcagg tgatgttctg atcaacagcg
    23761 gagatccatt aaggatctcc gtgagactat agaatgcctg atgcgctacg ctcatcaggc
    23821 atacaggact tccgccacta cattaaggaa aagttatggt atccgcactc atcaccgtcg
    23881 ccgttatcgc ctggtgtgcg caactggcct taggcggctg gcaaatttct cgttttaacc
    23941 gtgccttcga cacactatgc cagcaagggc gggttggcgt gggccgttcc agcgggcgct
    24001 ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca gcagcgcatc gtcgacacct
    24061 tgtttatgaa aggactgacc gtcttcgccc gaccgcaaaa aattcccgca attaccggta
    24121 tgcatgcggg tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg
    24181 ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg
    24241 cgaagttatc attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc
    24301 aggccgccat tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc
    24361 aatactttga caccacaggc acaaccattc gcaaagatct ggtcattctg gaacatgccg
    24421 gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccta
    24481 tcgatcataa aacactcatc aacacccaca agaaagagct gattgcagaa gctgccgtta
    24541 gttttatcca tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg
    24601 ttcccctgct ctcgcgcttt aataacatca cggtgatgac caacagcctg catatcgtca
    24661 atgcgctatc cgaactggat aacgaacaaa ctatcctgat gccaggcgga acgtttcgca
    24721 aaaaatcggc ctcatttcac gggcaactgg cagagaatgc cttcgagcat ttcaccttcg
    24781 ataaattgtt tatgggcacc gacggcatcg atctcaatgc gggcgtaacc acctttaacg
    24841 aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg
    24901 actcatcaaa gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata
    24961 agctgattac cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga
    25021 tcgatgtgat cataaccgga gagagcaatg agtgaagcac tactgaacgc gggacgtcag
    25081 acgttaatgc tggagttgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt
    25141 gttcgcgccg ccaatatcat cctgcactgt gaaggcaaag tggtggtttc gggaattggc
    25201 aaatcgggcc acattggtaa gaaaatcgcc gcaacgcttg ccagtaccgg cactccggct
    25261 ttttttgtcc atccggcaga agcgctgcac ggcgatctgg ggatgatcga aagccgcgat
    25321 gtgatgctgt ttatctctta ctccggtggc gcgaaggaac tggatctgat tattccgcgt
    25381 ctggaagata aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc
    25441 ctggcggcaa aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt
    25501 gcgccgacct ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc
    25561 atgcaggcgc gcggatttaa tgaagaagat tttgcccgct cccacccagc cggggcactg
    25621 ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccacag
    25681 gtggcgttaa ccgccagcgt gatggatgcg atgctggaac tcagccgcac cggtctgggg
    25741 ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg tctttaccga cggcgattta
    25801 cgtcgctggc tggttggcgg cggcgcactc accacgccag tcaatgaagc gatgacggtc
    25861 ggcggcacca cgttgcaatc gcaaagtcgc gccatcgacg ccaaagagat cctgatgaag
    25921 cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca aactcaccgg cgcaataaac
    25981 ctgcaggatt tctatcaggc cgggattatt taatccttca atcccagacg tttcgccagc
    26041 cgatgcaggt tggcgacgtc ggtttccagc atccgcgcgc aggcagccca gttgtgatga
    26101 ttttgtgcca gtgcctgacg aatagtttca cgctggaacg cttctgtcgc ttcacgcagg
    26161 ttttgcttaa caacgggcac cgccgccact tctggcgtcg gcaacgtcac ctcaggaaaa
    26221 gcaaaatgtt gcgcctcaag aatcacttca tcgccgctgc gggtggctct cgccagaact
    26281 accgcccgat gaatagcatg ttccagttcg cgcacgtttc ccggaaaact gtagtgttgc
    26341 agtaaatttc gcgctccggc acttaatacc acgcgggaga gcccctgccg caaacgacac
    26401 tgctcgcaga aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc
    26461 accgaaagtg gaaacacgct caggcgatga aacaaatcgg cgcggaatcg ccctgccagc
    26521 acctcttcgc gtaaatcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaacaa
    26581 cggtcatcgc caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc
    26641 aatgccaacg acaactcgcc gatctcatcc agaaacagcg tgccgttatc cgccatttca
    26701 aacttcccgc tgcgattact gatagcgcca gtaaacgctc ctttcacatg cccgaacaac
    26761 tcactttccg ccacactttc cggcagtgca gcacagttga gatagaccag cggattcacc
    26821 gcccgtggcg aggcttcatg aatcgctttc gccaccagct ccttaccggt tccagtctca
    26881 ccgctgatca ggacgttgag atcggacgcc gccacaatct caatctcttt tttcagttgc
    26941 gtcatgccag gggacaagcc aatcatctgc gtctgtttca ccgcttcaaa cggcgtggca
    27001 tcgcctggca gcatattctg gctttccagt tgttcaatca gcaacgcatt gcttaacgct
    27061 cccgccgcca gcgcagcaat cagccgtagc tcttcgtcgc tgaaaacatc gaactgatcg
    27121 ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt gcccggcaaa caatggcaga
    27181 ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc atagggatcg
    27241 ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg cgcgggcaat cgcttccagc
    27301 cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat cctttgccag accgtcgatg
    27361 gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc gcactccagc
    27421 acctgacgta gcgtggtgat caggcgctga aaacgatcct ggtgaccaat cccacgctgc
    27481 aattcgatgg cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca
    27541 atttgactat agatattgtc atatcgacca tttgattgat agtcattttg actactcatt
    27601 aatgggcata attttattta tagagtaaaa acaatcagat aaaaaactgg cacgcaatct
    27661 gcaattagca agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg
    27721 tggtgaaaaa taacattcat tgggttggtc aacgtgactg ggaagtgcgt gattttcacg
    27781 gcacggaata taaaacgctg cgcggcagca gctacaatag ctacctcatc cgcgaagaaa
    27841 aaaacgtgct gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc
    27901 gtaatgaaat cgatctggcg gatatcgatt acatcgtgat taaccatgca gaagaggacc
    27961 acgctggggc gctgaccgaa ctgatggcac aaattcccga tacgccgatc tactgtactg
    28021 ccaacgctat cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg
    28081 tgaaaactgg cgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacac
    28141 caatgctgca ctggccggac agcatgatgg cttacctgac aggcgacgcg gtgctgttca
    28201 gtaacgatgc tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc
    28261 agacggagct tttcgagcag tgccaacgtt actacgccaa tatcctgacg ccgttcagcc
    28321 gcctggtaac gccgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag
    28381 ccacttccca cggcgtggta tggcgcgata acccgacgca aattgtcgag ctgtacctga
    28441 aatgggcggc tgattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata
    28501 acacccgcat gatggctgac gctatcgccc agggaattgc ggaaaccgac ccacgcgtgg
    28561 cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc
    28621 gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg
    28681 ccgggctggt ggaggagatg accggattac gcttccgtaa caaacgcgcc agtgctttcg
    28741 gatctcacgg ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg
    28801 gtttcgaaat gtcgctcagc ctgaaagcca aatggcgacc agaccaggac gctctggagt
    28861 tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga
    28921 gcacggtgaa ttcggtggtt aaagaagaaa cctctgccac cacgacggct gacctcggcc
    28981 cacggatgca gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc
    29041 aggacgttgc gccaggaacg ccgtggagtg aagttccgga taacttcctc tgcccggaat
    29101 gctccctcgg caaagacgtc tttgacgaac tggcatcgga ggcaaaatga gcaacggcat
    29161 tgtgatcatt ggttcgggct tcgccgcccg acaactggtg aaaaatattc gcaaacagga
    29221 cgccactatt ccattaacct tgattgccgc cgacagcatg gatgagtaca acaaacctga
    29281 catcagccat gttatcagtc aggggcaacg tgccgatgac cttacccgcc agacggcggg
    29341 tgaatttgcc gagcagttta atctgcacct gtttccacaa acctgggtga cggatatcga
    29401 tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg caatacgaca agctagtact
    29461 ggcaaccggt gccagtgcct ttgtcccgcc tgtgcctggg cgtgagttaa tgctgacgtt
    29521 aaatagtcag caagagtatc gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt
    29581 gattgttggc ggtggtttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa
    29641 agcggtcacg ctaatcgaca acgctgccag tattctggcg tcgttaatgc caccggaagt
    29701 aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt catctgctgt tgaaatctca
    29761 gttacagggg ctggaaaaaa cggattctgg cattcaggca acgctggacc gccagcgcaa
    29821 tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc ccggaaaccg ccctggcacg
    29881 acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa
    29941 taccgatatt tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct
    30001 ccagccgatt caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc
    30061 gctgaaactc ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc
    30121 aggcgaaacc cagcgtcagg atttacgctg gcaaattaat accgaacgcc agggaatggt
    30181 ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg aggatcggat
    30241 gaaagaggca tttggattgt tgaaaacatt gccgatgtag gtgggctact gtgcctaaaa
    30301 tgtcggatgc gacgctggcg cgtcttatcc gacctacggg gacgcatgtg taggccggat
    30361 aaggcgttta cgccgcatcc ggcaatggtg tccaaatgca acacgtttta tccgttctgg
    30421 acttcacccg ctaaccaacg cgccgcagca ataaccccct gccccagaga caaaccgcca
    30481 tcacccgccg gtaaactctg tggaaagagc aatgtgaaat cagcgagata atgcgccaga
    30541 cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa ataccagcgt agtgatacca
    30601 cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct gcgccagcgc atcatgaaac
    30661 gcccacgcgc gttgattaac cggtgcctgc cagttcagcc actgctgcca gaaagtggcg
    30721 agatccagtt gattgtccac ccgcggcatt gtcaccggat gcgtcactcc gtggcatgag
    30781 gctgcgagcg cctccagagc acaagccgct tcaccttcat aacttaacgt ggctggcgca
    30841 cagcccagtg ccgccgccac tgcatcgaaa aaacgcccac acgatgacgc cagcggcgcg
    30901 ttaattccac gctcaatggc ccgcgccagc acgctccagt tttgctgttg cacactcgct
    30961 gtttcgggat aattctgcca ctccggcaca aagcgcaggc actgcgccag caggtttcgc
    31021 cacggctgct tcgccgccaa atcgccacca gcaagcgcca ctgcaggcaa gccgcccagg
    31081 tgctcacatt cgcgatagtt cacccgcagg cactcgccgc cccacaaagc gccattctcc
    31141 cccataccga taccgtcgag cgtcaaagca atgacatcac cgccatccag cggccactga
    31201 tgctctgcca gacacgccgc tgcgtgggca tgatgatgca gcaccgtttg cgtcggcaga
    31261 ttcatttcgc gcgcccactg gctggagaca tagcccggat gtgcgtcatg cacaacgtat
    31321 tgcggggtga aatcgtagat gttttgcatc aggcgtaacg cttcgcgcca ctgcatctgg
    31381 atgccatcgt cacttaaatc gcccagatgc tgactcaaca ccgcttgttc accgcgcacc
    31441 aggcagaagg tatttttcag atccgcgccg agacacagca caggcggaac atttttaaag
    31501 cccggaggca aagccagcgc atccggcaca tacccccgcg aacggcgcag catttcgccg
    31561 ctttcgcgca ccaccgaatc atccatccgc tgcacgatgt cgcggttatg tatcaaaaat
    31621 ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt tgctgatagc tggtggttta
    31681 ccgctcaggt tgccggaggt catcaccagc gggcattgca gttcctgtaa cagcaaatgc
    31741 tggagcgggt tcgcaggcaa cattaccccg acttcgttaa ggtcaggggc gatatcatca
    31801 caaagctcag gaacgtattt tttatccacc agcacaatcg gcgcggcggg cgtggtaagc
    31861 aactggcgcg cagcgtctgg taaaccgtca gccactggca acatgaccgc cagcggtttc
    31921 gccgggcgat gtttgcgcgc ccgaagtgtc gccaccgcgt tactgttacg tgcatcgcag
    31981 gcaagatgaa atccgccaat ccctttgatg gcgacaattt tgcccatttt taactgtgcg
    32041 atagctgcct gtaatgccgc ctcttgttcc gcatgttcac catgacttac ccattcaaga
    32101 tgcgggccac actccgggca ggccaccggc tgggcgtgga agcgacgatc gagcgggtca
    32161 cggtactctt tgtcacaggc cggacatagc ggaaacgccg ccatcacggt aaacgggcgg
    32221 tcgtaaggca tggcgcgaat aatggtgaaa cgcgggccgc agtgggtaca gttgataaac
    32281 ggataacgat aacgccgttc gcctggggta ttcatttcgg caaggcaagc agggcaagta
    32341 gcggcatcgg gaacaatttg cgtattcatg gtgccgcctg tgctctggcg tatagtgaac
    32401 tcggtgggca gttgtgacca gataaacggc tcacgctcga cgctatcaat acgcgccagc
    32461 ggcgggcagt gctgatacaa ttgaacaaga aacgtttccg ggtcttcccg cagccggact
    32521 tctacgccat cgccgtcatt acagacatcg ccgtgaagat ttaattgctg tgccagctgc
    32581 cagacaaacg gacgaaaacc gacgccctgc actttgccac gaatacgcag ttggacaccg
    32641 caagatgtgt tttttgccat tgagttattc ccgccatcat gaattgcgta acccgccctg
    32701 ccggacacga cagcgtcgca tccggcagtc acaggtcggc gataccgccc gctccgtatt
    32761 ctacgaatat ttccgggaat tcctttgatg ccagaacagt tctgtaagat ttttagaaca
    32821 tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc aagtttatta cgatcgacac
    32881 aaatcagcgc atgagtcggg caagccgcca tacacgccgg gccgtcttca cgatggttgc
    32941 acaggtcgca tttattggct tcggctttgt cagcccgtac attcagaccc gcgccgctgt
    33001 tgcggatcac cggacgtacc accacttcca tcgcaccata cgggcaagcc acaacgcagg
    33061 ttttgcaacc aatgcaacgt tcctgcatca catgaacaaa ccctttatca cggctgatag
    33121 caccattcgg gcagacgtta gcgcacggtg catcttcaca ctgacggcaa actgtcgccg
    33181 tggaaatgtt cacaccttta atgacatgga tacgcggtaa aaaagtttcc ggggtcagcg
    33241 atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc tacttcacag gtacggcaac
    33301 caatacattt actcgcgtca gcaatgatga aacggttcat caaattctcc agcaatgaca
    33361 gttaatgcgc cgatacattc acaaatcatg ccagttttta atttactgtt atttaaggaa
    33421 attaatttct gtaatgcagg aaaaacgatg tcatcgacac tagtgacgat gacatgtgat
    33481 gacaatgttt atcgtgaagg agcaatgagt gagtcgcggc ggatcagttt tccgctgaag
    33541 gttttcggcg gtgagaaatc cccgccatcg agcataaaaa tcagccgtcc aataatttcc
    33601 tgaatcatct cagtcaccgg aatttttacg ctggagagcg ccggaacggt gtagggggca
    33661 atagcgatat catcgaatcc gataactgac acctgctctg gcaccgctac gccgcgctcg
    33721 tgtaacgctt tcatcgcacc tatcgccata tcgtcgttac tggcaactaa cgcgctaaat
    33781 ttagccccac gttcgagcaa catttctacc ccttcggccc cgctggcagg cgtccattta
    33841 ccgttagcga taagtttttc attgagcgca ataccatgct gcgccagcgc gtctttatac
    33901 ccggcaagac gttcaatgct ggtgggggaa tccatcgagc cggtaaggaa agcaatctcc
    33961 tgatgcccgg cgtttatcaa ctctgccacg gcgttaaaac tggtctgttt atgatcgcac
    34021 cagacgctat ggctgctgtt tttgcgcagg cggcgattaa gcaccattat cggctgactg
    34081 tgcgcgtcaa tgatgtcatc gatctcatcc acgcttaaaa aacgcgggta aatcatgatc
    34141 gcgtcgcagc gcagatccag cagatactga atcgcctggc gctcttcttc tgcgctgtgt
    34201 ttaccatctg ccaatagcaa ctgccgccct ttctcttccg ccattcgcgc ggcatgaaag
    34261 agtaattcac taaaataaat gccgtggtaa agcgtgttgg tcactaccag ccccagcgtc
    34321 tgagtactct tcgccgacag attgcgcgcc agcaagtttg gacggtaacc gctctcttct
    34381 accgcctgaa acacgcgatc tttagtctcc tggctgacgt agccattacc tgaaagcacg
    34441 cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca cttccagcat cgtcgtcatc
    34501 attttcatcc ctttacacgc aatcaacgca gtgtactgca ccgtttgccg attgtccttg
    34561 cacaatcggc gggaaaaata ttcaggtgac cggtttcaca aatataaaaa atgaacaatt
    34621 cactctcttg cttatttagt gacaactatt catgattttg tgaaaccggt ttcttaattc
    34681 cgtttcagca tcggcatttt tccgtcacgt cgactgataa caactacatc taccctactg
    34741 ataacaggat aaaatccgat ggccaaaaat tatgcggcgc tggcacgctc ggtgatagcg
    34801 gcactgggcg gcgttgataa catctcggcg gtcacgcact gtatgacgcg gttgcgcttt
    34861 gttatcaaag atgatgcact tatcgacagc ccgacgttaa aaaccatccc cggcgtgctc
    34921 ggcgtggtac gtagtgacaa ccagtgtcag gtgattatcg gcaataccgt ttcacaagcc
    34981 tttcaggaag tcgtcagcct gctgccggga gatatgcagc ccgcacagcc cgtgggtaaa
    35041 cccaaactca cgctacgtcg cattggtgcg gggatcctcg atgcgctgat cggcaccatg
    35101 tcaccgctga tcccggcgat tatcggcgga tcgatggtca aactgctggc aatgatcctc
    35161 gagatgagcg gcgtgctgac aaaaggatcg ccgaccttaa ccattctgaa tgtgattggt
    35221 gacggtgctt tcttcttcct gccgctgatg gtcgcggcat ctgccgccat caaatttaaa
    35281 accaatatgt cgctggcgat tgccatcgcg ggtgtgctgg tacatccgag ttttattgaa
    35341 ctgatggcga aagcggccca gggtgaacat gtcgaatttg ccctgattcc ggtcaccgcg
    35401 gtgaaataca cctacacggt gatcccggcg ctggtgatga cctggtgcct gtcatatatc
    35461 gaacgctggg tggacagcat tacaccggcg gtgaccaaaa acttcctcaa accaatgctg
    35521 attgtgttga ttgccgcacc gctggcaatc ctgctgattg gcccgattgg tatctggatc
    35581 ggtagcgcca tttcggcgct ggtttacacc attcatggtt atctgggctg gctttcagtc
    35641 gccattatgg gcgcgctgtg gcctctgctg gtaatgaccg ggatgcaccg cgtctttacg
    35701 ccaaccatca ttcagaccat tgccgaaacc ggcaaagaag ggatggtcat gccgtcagag
    35761 atcggcgcta acctgtcgct gggcggttca tcactggcag tggcgtggaa aacgaaaaac
    35821 ccggaactgc gtcagacggc gctggctgcg gcggcatcag ccattatggc ggggatttcc
    35881 gaaccggcgt tatatggcgt ggcgatccgc ctgaaacgtc cgcttatcgc cagtcttatc
    35941 agcggtttta tttgcggcgc ggttgccggt atggcggggc ttgccagcca ctcaatggca
    36001 gcgccggggc tatttaccag cgtgcagttc ttcgatccgg cgaatccaat gagcatcgtc
    36061 tgggtgttcg cggtcatggc gctggcggtg gtgctgtcgt ttatcctcac actgttgctc
    36121 ggctttgagg atattcctgt tgaggaagcg gctgcccagg cgcgaaagta tcagagcgta
    36181 caaccgaccg tcgccaaaga agtaagtctt aattgaggat gaaaatgtca gtatttccag
    36241 aaagtttttt atggggcggc gcgcttgccg ccaaccagtc tgaaggtgcg ttccgtgaag
    36301 gtgacaaagg tctgaccact gtcgatatga tcccacacgg cgagcatcga atggcggtga
    36361 aactggggct ggaaaaacgt tttcagttgc gagatgacga gttttatccc agccatgagg
    36421 cgacggattt ttatcatcgt tataaagaag atatcgccct gatggcagag atgggattca
    36481 aggttttccg tacctcaatt gcctggagcc gtctctttcc gcagggcgat gaaatcacgc
    36541 ccaatcagca gggcattgct ttttatcgtt ctgtctttga agagtgtaaa aagtacggta
    36601 tcgaaccgct ggtcacgttg tgccacttcg atgtgccgat gcatctggtc accgaatatg
    36661 gctcctggcg taaccgcaag ctggtggagt ttttcagccg ctacgccaga acctgctttg
    36721 aagcatttga tggtctggtg aaatactggc taaccttcaa tgaaatcaac attatgttgc
    36781 atagcccgtt ctccggcgcg ggtctggtgt ttgaagaagg tgaaaatcag gatcaggtga
    36841 aatatcaggc cgcgcatcac cagctggttg ccagtgcgct agccaccaaa atcgcccatg
    36901 aggttaaccc gcaaaatcag gtgggctgta tgctggcggg cggtaacttc tacccttaca
    36961 gttgcaagcc ggaagatgtc tgggcggcgc tggagaaaga ccgggaaaac ctgtttttta
    37021 tcgatgtgca ggcgcggggc acgtatccgg cttactctgc ccgcgtattc cgcgaaaaag
    37081 gggtaaccat caacaaagca ccgggcgatg atgaaatcct gaaaaacacc gtcgattttg
    37141 tctctttcag ctattacgcc tcgcgctgcg cctcggcgga gatgaacgcc aacaacagca
    37201 gtgcggcgaa cgtggtgaaa tcgctgcgta atccgtatct acaggtgagc gactggggct
    37261 ggggaattga tccactcggt ctgcgtatca ccatgaatat gatgtacgat cgttatcaga
    37321 agccgctgtt tctggtggaa aacggcctgg gcgcaaaaga tgaatttgct gccaatggcg
    37381 agattaacga cgactatcgc atcagctact tacgcgaaca tatccgcgca atgggcgaag
    37441 cgattgcaga cggcattccg ctgatgggct acaccacatg gggctgtatt gatttagttt
    37501 ccgcctctac gggtgaaatg agcaaacgct acggctttgt ctttgttgac cgtgacgacg
    37561 caggcaacgg tacgctgacg cgcacgcgta agaaatcatt ctggtggtat aaaaaagtga
    37621 ttgccagtaa tggggaagat ttagagtagg taacagtgcc ggatgcggcg tgaacgcctt
    37681 atccggccta catgtcgcac gaaccatgaa ggcccgataa gcgcagcgca tcgggcaatt
    37741 tagcatttgg catcagagct ggttcacccc atcaagaaca tccctgtcct gattccttaa
    37801 tgaaaaacta ctcttcttcc accgctaact gcgcgaagcc gccatttcct tcccagcctt
    37861 ccagtcgttg ataaacggtt tctaccgcat ctttaatcgg ctgggtcatc gggtagtaaa
    37921 agccgacgat atccggctga atgccgagga aaatcacttc gccaatatct tctttcaact
    37981 ggtcgataag gtaattcaac ggcatgttat gggtagtcat cataaacatc tcggcgatat
    38041 catccgggtc gatgatgcgg atctcgccgg ggtttagccc catatccgtg gcgtcgacaa
    38101 tcagcagtcg tgtcgggcgc agttcacgga tagcgacgat gtcgttttcc ggtgcgctac
    38161 cgccgtcaat caccacccag ttacctttcg gcgcggcggc gcacttttcc gccagcagcg
    38221 gacctgcgcc atcatcgccc atcatgctat tgccaacaca gagtaaaacg tcagtcacgc
    38281 agtctcctca ccatcaaata gatggcgttt tcctgatgaa tatcatgcag catacttaac
    38341 agcattgtac tccattcctg ctgctgcgaa gtttgcgctg cccgggcttt atcgaatgcg
    38401 ttggccagca tcggcacatg gttaatgtcg atgacgatct caccatattt cggcacgcct
    38461 tccattttgc ggcgggcttc actgcctgcc tccagagtgg caatccatgc cagatattca
    38521 tcccacgggc aggtgagcgc cgcttccagg caatcgataa ccccaaggtg gtgaccaatc
    38581 gccaggctgt aatagaccac ctgctgcgcc tcggcgggcg tggcatcgtt ctcatcaata
    38641 aatttacggc tcagttgact gaacaccacc ttttcactca tcggatacgc gcctcttcaa
    38701 caacatgatt cagatggctg acaatctcgt tcagacgcgg atcgttttcc gcttccagcc
    38761 agcgtgcaac ctgttcttcg ccctgcccta actgtgtaag gtaatcatcg gcaatctgac
    38821 gaccgtaacg ataacccgcc aggcgacgtg cttcgcgatc cactttcacg cgcagcggct
    38881 gcaccatatc gccatgcagg atctccgccg gttgttcatc cagttcaccc ggcccacggg
    38941 cgtgaatttt ctgctccagc aggccgagcg ccattgcaaa gccgtacagc gtggcggcag
    39001 gcgttggcgg gcagccaggg atataaacat ccacagggac aattttatcc gtaccgcccc
    39061 acacgcagta gagatcgtgg aagatcccgc cgctgttacc gcaggcaccg taggagatac
    39121 aaattttcgg gtccggcgcg gactgccacg cacgcagcgc aggggatcgc attgcacggg
    39181 tgaccgcgcc ggtaaacagt aaaatatccg catgacgcgg tgaaggaacg actttaatgc
    39241 cgaagcgttc tgcatcaaac agcggcgata aagtggcgaa aatttcgatt tcgcaaccgt
    39301 tgcagccgcc gcagtccacg cggtaaacat aggcagaacg tttgattttt ttcagtaacg
    39361 acgccttcat gctggcgatg gattcatcca ccgtcatggg gaccggaatg ccgttggcgt
    39421 cacgggggcc taataaattg ctcatcagat ggcctctttc atatggcgag tcagttcaat
    39481 acggtcggac ggcaccaggc atttctggcg cttacattcc gggcaagtct caaagctttc
    39541 gcggtggttt tccgcgcggc tgtcgccgtt gtgcttaagc agcgcaatgg cgtagtcgat
    39601 ctctttctgg acggcgaaag gacgattgca gacgcggcag ttgcacagcg cgaagcggga
    39661 ctgttgcagg aagtcttctt tcttccacac cgccagttcg tactcttgcg acagtttgat
    39721 cgccgccgtc gggcagactt cttcgcagcg tccacagaag atgcagcgcc caagattaaa
    39781 ctcccaggca agctctcctg tggcgaggtc agtttcaacc gttaaggcgt ttgacgggca
    39841 ggcattgacg caggccgcgc agccgatgca ctgctgcggg ttctgctctg gcttaccacg
    39901 gaagttttta tcaaccgcaa tcggctccag cggataagac gaggtcgccg tgccggtttt
    39961 gatgactttt ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg
    40021 ctcaatgctg taacgctcga gttctttgta cggcaccact ttgctcttct tcttacgcac
    40081 atcgaccacg gtcatgcggt cggtacagga gtagcaaggg tcgaggctac cgataatcag
    40141 cggcgcatcg gaaacggtgt tgccgcgcag catgtagcgc agggtcggcc agttcgcgta
    40201 ggtcgcggca cggcagcgcc agcggtacag cttctggttg tcgccggtca tgctccagtg
    40261 gatatcatcg ccgcgcggcg cttcggcaaa gcccagcgca aagcggtgcg gaatgtaggt
    40321 aaagccttcc accatcagtg ggccacccgg caggttatcc agaccgtagt cgatcatgtt
    40381 cagcgcggta tagacttcgt tgatacgcac tttcagacgg gaaataacgt cgcagccctg
    40441 ctcgctgtgg acttccattg gcagcaggcc atagccgaca aacgggtgat cggcgcgggt
    40501 atcacgggcg tgaccgctgg cacggaccat cgggccgacg ttactgaagt cgcgagcgat
    40561 ttccgggtcc agacgaccaa tgccgacagt gcgctgttcc atgttcggag tgctcagcag
    40621 cacatccacc agctcctgca cttcacgacg catctgttgt gccagctggc gggtctggat
    40681 catgtcgtct ttcagcagat cgcgacgaat cccgccgatc aagttcaggc cgtaggtttt
    40741 acgcgcaccg gtaaggatct ctgccatttt catggaggtt tcacgcacgc ggaagaactg
    40801 cataaagccg gagtcgaagc cggtaaagtg acaggccagg ccaaggttga gcagatgcga
    40861 gtgcaagcgt tctacctcca gcagaatggc gcggatcatc tgcgcacgtt ctggcacctg
    40921 aatacccatc gcgttttcca ccgacgtggt gtaggcggtg ctgtgggcaa agccgcagat
    40981 cccgcacaca cggtcagaga ggaaggtcac ttcgttataa cccatacggg tttccgccag
    41041 tttttccatg ccgcgatgga cgtagaacag acggtagtcg gcgtcgataa tgttttcgcc
    41101 atcgacgaac agacggaagt ggcccggttc atcagaagtg acgtgcagcg gaccaatcgg
    41161 cacgacgttg tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt
    41221 ggtcggtgcc ggacgctgac gataatccat gctgtcttta cgcagcggat aaagttcatc
    41281 cggccagtca tccggcagca ccagacgacg ttcatccggc agaccaaccg gaatcaaacc
    41341 gtacatatcg cgcacttcac gctcgcccca caccgccgcc ggaacgcgcg gcgtcacgga
    41401 cggatattcc ggtttgttgg cgtcaacttc gacgcgaacc gtaatccaac acttagtgcc
    41461 cttctccatc gacagcacgt agtaaacggc gtaatgacca ttcagtttgc gttcgtcgtt
    41521 accaaacagc accgacagcc agccaccctg tttgtagtaa agaaactcca ccacttccgg
    41581 caggtagttc acctttacgg tgacagtcag ctgatctttg gtctgccagg cgtggtccag
    41641 cacgacgccc ggaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc
    41701 agacatgctc aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca
    41761 aagccaaacc cagcccaggt aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg
    41821 ttttcgaaca gcgcgataac caggacgccg accaccagtt ttacgatggc aatcaccagc
    41881 gccagcagca gtccaccggc ggtgaaggtt tccatttgtc cccacggaat aaacaccccg
    41941 acgaacatct gcaacaccac cagctgtttc aggctgatac cccatttcat gacgccaaag
    42001 ccgctgccgc tgtattcaga gagcgggcct tcctgcagct cctgctcggc ttccgccagg
    42061 tcgaacggca gtttgcccat ttcgataaag gtggcgaacg cacaggcaca aagcgccagt
    42121 accagcggga tgctctggct cagcggccag tgataaacgg tgtcggtgat gttgctgatg
    42181 ttggtggaac cggcaacctg tgcggcaacc cacagaccaa gcagcagcat cggttcgacc
    42241 agcacgccaa gcatcgcttc acggctcgcg ccgatagcgg taaacgggct accggtatcc
    42301 agaccagaaa tggcaaagaa gaaacgcgcg atggcaaaga gatacagtaa ggtgatcaaa
    42361 tcacccagtt gcggcagcgg agaaccgacg gtcaccaccg gcagcgcagt agcgatagtc
    42421 agcatgacgc ccaccatcac atacggcgtc aggcggaaca cccagccgga ggcatccggg
    42481 ccaacgctct ggcggcccag cagtttgaaa atgtcacgat actcctgcaa cacacccggc
    42541 ccacgacggt tatgcagacg ggcgcgcgcc gcgcgggtaa taccggagag cagcggcgca
    42601 acggcaaata acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc
    42661 gtgaaacaat aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca
    42721 acgcactacc ttcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgca
    42781 gtttcagcac tggcgcaaac gcctgtttca ccggcatggc aaaaccgtga gcggtaatca
    42841 ccattgattt ttcgtggtcg tagccgcaca cccaggccgc gccgcgggaa cgcgatggca
    42901 aacgatcgcc tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca
    42961 gcaacgtgat catcggttga gaaacggtgg tgttagcagg ctccagcggc agaggtacag
    43021 cagcagacag catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg
    43081 ccagtgccac tacgcttacg ctcatcagga gcggtgcaca ggtggcgttt tcggcttctt
    43141 tggtacgcgg cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca
    43201 gcgcaccggt aattgccaga cccacggcga gcagcggccc cagcagacgg gcaacaaacg
    43261 cgccactatt gctcagtttg aagaaggatt gatagataac ccattccccg gcaaaaccat
    43321 tcagcggcgg cagcgcagcc attgccatca gcccgactaa catggcgatg gagataaccg
    43381 gcattttctt gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga
    43441 tgctccccgc tccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca
    43501 gaccaccgac caggccaaga gcaatcagtg ccggttgttc gagcgcgata cccgttacgc
    43561 cagcgcccag ccccagcagg ataatgccga tattttccag ggtgtggtaa gccagcaggc
    43621 gctggatgtt gtgttccatc aacgcataca gaccaccgac gaacgcggtg atcatgccga
    43681 gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt gacagggtta
    43741 aaatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca gctggcgcag
    43801 aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatcccg gccagcaggc
    43861 caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt tgcatacgca
    43921 tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag caagcaatcg
    43981 ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct ttgctgttgc
    44041 tggtgaggaa caccgcgcac agggccatga tttcggccat taccacgaac atgccgaggt
    44101 tgctggcaat gacggcgcag acggcggcag ccatcaacat attgatctgc aagccgttgc
    44161 acttcacctg cgcgtggcga tgccagtcaa tgttgtagag gctgacaaac agaccgcaca
    44221 gaccgagcgt aatcagccaa atcgcgttaa gcggagaaat ttgcacatcg tagcttacca
    44281 gcgacagcgc accgctcacg ccaaccgcgc cagtcagtac agtgaagccc gcggctgccg
    44341 tatacagact accaaccgcg ccgccaattc cagctatcca gccacttaac gctttttgaa
    44401 aagaaaagag aaatgccaga acagcggcgg cgacaaacca cgccacgccg ctattgatca
    44461 gggaaattgc gctcatttag cctctccact ttgagcctgc tgaaacaagg tgagatcgcc
    44521 aaagtccgtg ttaaaagtca gctcacgctt acgtttgctg acgcgggcga tatcagtgtt
    44581 atccaccaga tgcagggctt tagtcgggca catccgcacg caggccggac cttgttcatc
    44641 aaagctacaa aggtcacatt tgacggcgat cgcgcgaata cctggcaccc agtcaagcaa
    44701 tgtgctgaca cgcgccggag caggcggtgc cggtggcgct ttcggggtat tggcgtttgc
    44761 cggaatatcc agcggacggc tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc
    44821 gcacagcttg cagcttacgc acaggctttc attcaactgc acggccccat caacgcgggt
    44881 gatggcgtta accgggcaga ccaccgcgca gggtgcatct tcacagtggt gacagagctg
    44941 cggcgcagat tctttttcat tcagcatcac tctcaggcgc ggcattgatt gcaggccgtg
    45001 ctggcgatgc gtctctgaac aggcggcctc acaagtgtgg cagccgatac agagcgtgga
    45061 gtcagcaatt acaaaacgat tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa
    45121 acatgccgat gaaatgtcat tttcgacact catcgacacg cccatcccca aacaggcgta
    45181 acgcctgcaa aacgggcaaa gcctcagctc atgctgccgg gctttgtccc tttaccagtt
    45241 ggcttaaatt caccggcaca ttgttttcaa tagcggtgta taagctgtcg taaacgccag
    45301 cgattttttc gagatagtgt tccagaacct gttcgcgatc gcggatgttg accttgccat
    45361 caatcatgcc gtcaatgctc agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt
    45421 ttgtttcgac gtaatactcg atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa
    45481 tgcgaaaatg ttcaaaaagg acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt
    45541 gatgcaggcg cgctttcgat aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc
    45601 actggtcctg taggcgacga tgccgctgtg cgatgtaatc ggctttctcg cttatttccc
    45661 aaatagtcat tgtcaggtta cccgtttaac agagatgcca gctttaagca ttttttgtgc
    45721 caacttttaa tttattgtta ttgaagagat ttttaagcta aagatgaatt tcgtcgccgt
    45781 gtcgacgtgt catttcgaca tcatcgacat tattcaccgc agggataatc aacactggca
    45841 caattattgc ttgtagctgg caatagttaa tgggaggcga tatgcacgaa ataaccctct
    45901 gccaacgggc actggaattg atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa
    45961 ctggggtctg gctcaaaatt ggcgcatttt cttgtgtcga aaccagctct cttgcctttt
    46021 gttttgatct ggtttgccgc ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac
    46081 aagaggccga atgctggtgt gaaacatgcc aacagtatgt gacgctactg acccagcgcg
    46141 tccgccgctg tccacagtgt catggtgaca tgctgcagat tgtggcagac gacggtttac
    46201 agattcggcg gatagaaata gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg
    46261 aaggcaacct gtatatcgag ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc
    46321 catttgcccc ggcggcacgc ccgaagatga aaatcaccgg cattaaagcg cctgaattta
    46381 cccccagcca gactgaagaa ggcgacctgc attacggtca tggcgaagcg ggcactcacg
    46441 caccgggcat gagccagcgt cggatgctgg aagtcgaaat tgacgtgctg gacaaaaata
    46501 accgtctggc tgaacgcaac cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc
    46561 tggtttccag ccctggttcc ggtaaaacca ccctgctgac ggaaacccta atgcgcctga
    46621 aagacagcgt tccgtgcgca gttattgaag gcgaccagca aaccgtgaac gatgccgcac
    46681 gcattcgcgc taccggcaca ccagcgattc aggtgaatac cggtaaaggc tgccatcttg
    46741 acgcacagat gattgccgac gccgcaccgc gcctgccact ggacgataac ggtattctgt
    46801 ttatcgaaaa cgttggcaac ctcgtatgcc cggccagctt cgatctcggt gaaaaacaca
    46861 aagtggcggt gctttccgtt accgaaggtg aagacaaacc gctgaaatat ccgcatatgt
    46921 ttgccgccgc ctcgctgatg ctgctcaaca aagttgacct gctgccgtat ctcaactttg
    46981 acgttgagaa gtgcatcgcc tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta
    47041 tttccgccac cagcggcgaa gggatggacc agtggctgaa ctggctggag acacagcgat
    47101 gtgcataggc gttcccggcc agatccgcac cattgacggc aaccaggcga aagtcgacgt
    47161 ctgcggcatt cagcgcgatg tcgatttaac gttagtcggc agctgcgatg aaaacggtca
    47221 gccgcgcgtg ggccagtggg tactggtaca cgttggcttt gccatgagcg taattaatga
    47281 agccgaagca cgcgacactc tcgacgcctt acaaaacatg tttgacgttg agccggatgt
    47341 cggcgcgctg ttgtatggcg aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg
    47401 gaacaggtga tgcagttaat tgagcatctg cgcgaacgtg cttcacatct ctcttacaca
    47461 gccgaacgcc ctctgcggat tatggaagtg tgcggtggtc atacccacgc catttttaaa
    47521 ttcggcctcg accagttact gcctgaaaac gttgagttta tccacggtcc gggttgcccg
    47581 gtgtgcgtac tgccgatggg tagaatcgac acctgcgtgg agattgccag ccatccggaa
    47641 gtcatcttct gtacctttgg cgacgccatg cgcgtgccgg ggaaacaggg atcgctgttg
    47701 caggcaaaag cacgcggtgc cgatgttcgc atcgtttact cgccaatgga tgcgttgaaa
    47761 ctggcgcagg agaatccaac ccgcaaagtg gtgttcttcg gcttaggttt tgaaaccacc
    47821 atgccgacca ccgccatcac tctgcaacag gcgaaagccc gcgatgtgca gaatttttac
    47881 ttcttctgcc agcatattac gcttatcccg acgctgcgca gtttgctgga acagccggat
    47941 aacggtatcg acgcgttcct cgcgccgggc cacgttagta tggttatcgg cactgatgcc
    48001 tataatttta tcgccagcga ttttcatcgt ccgctggtgg tcgctggttt cgaacccctt
    48061 gatctactgc aaggcgtggt gatgctggtg gagcagaaaa tagcggccca cagcaaggta
    48121 gagaatcagt atcgtcgggt ggtaccggat gccggtaacc tgctggcgca acaggcaatt
    48181 gctgatgtgt tctgtgtcaa cggcgacagc gaatggcgcg gcttaggcgt gattgaatct
    48241 tctggcgtgc acctgacgcc ggattatcaa cgattcgatg ccgaagcaca tttccgcccg
    48301 gcaccgcagc aggtctgcga tgacccgcgc gcacgttgtg gtgaagtatt aacgggcaaa
    48361 tgtaagccgc atcaatgccc gctgtttggt aacacctgta atcctcaaac cgcatttggt
    48421 gcactgatgg tctcctccga aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag
    48481 aatgaagcgt gaataatatc caactcgccc acggcagcgg cggccaggcg atgcagcaat
    48541 taatcaacag cctgtttatg gaagcctttg ccaacccgtg gctggcagag caggaagacc
    48601 aggcacgtct tgatctggcg cagctggtag cagaaggcga tcgtctggcg ttctccaccg
    48661 acagttacgt cattgacccg ctgttctttc ctggcggtaa tatcggcaag ctggcgattt
    48721 gcggcaccgc gaatgacgtt gcggtcagtg gcgctattcc gcgctatctc tcctgtggct
    48781 ttatcctcga agaaggattg ccgatggaga cactgaaagc cgtagtgacc agcatggcag
    48841 aaaccgcccg cacggcaggc attgccatcg ttactggcga tactaaagtg gtgcagcgcg
    48901 acgcggtaga taaactgttt atcaacaccg ctggcatggg cgcaattccg gcgaatattc
    48961 actggggcgc acagacgcta accgcaggcg atgtattgct ggtgagcggt acactcggcg
    49021 accacggggc gactatcctt aacctgcgtg agcagctggg gctggatggc gaactggtca
    49081 gcgactgcgc ggtgctgacg ccgcttattc agacgctgcg tgacattccc ggcgtgaaag
    49141 cgctgcgtga tgccacccgt ggtggtgtaa acgcggtggt tcatgagttc gcggcagcct
    49201 gcggttgtgg tattgaactt tcagaagcgg cactgcctgt taaacctgcc gtgcgtggcg
    49261 tttgcgaatt gctgggactg gacgccctga actttgccaa cgaaggcaaa ctagtaatag
    49321 ctgttgaacg caacgcggca gagcaagtgc tggcagcgtt acattcccat ccactgggga
    49381 aagacgcggc gctgattggt gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt
    49441 atggcgtgaa acgaaccctc gatttaccac acgccgaacc gcttccgcgt atatgctaat
    49501 aaaattctaa atctcctata gttagtcaat gaccttttgc accgctttgc ggtgctttcc
    49561 tggaagaaca aaatgtcata tacaccgatg agtgatctcg gacaacaagg gttgttcgac
    49621 atcactcgga cactattgca gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcaa
    49681 ctggtaaagc gttctgcgct cgccgacaac gcggctattg tgttgtggca agcgcagact
    49741 caacgtgcgt cttattacgc gtcgcgtgaa aaagacaccc ccattaaata tgaagacgaa
    49801 actgttctgg cacacggtcc ggtacgcagc attttgtcgc gccctgatac gctgcattgc
    49861 agttacgaag aattttgtga aacctggccg cagctggccg caggtgggct atacccaaaa
    49921 tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg tggctgtgaa
    49981 tttattcgtt atgacgatcg cccctggagc gaaaaagagt tcaatcgtct gcaaacattt
    50041 acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgttaa caatgtcgac
    50101 tatgagttgt tatgccggga acgcgataac ttccgcatcc tggtcgccat caccaacgcg
    50161 gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccattac
    50221 tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa caaactcaac
    50281 atctactcca ctcactatct tgataaacag catcccgccc acgaacagag cgaagtcgat
    50341 gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct gatcaatctc
    50401 cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacacctg gggcaaccag
    50461 attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctgaaa
    50521 ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttact gcgccagatt
    50581 gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat ccatcgtctg
    50641 aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt
    50701 gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa
    50761 atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac gggtaaagag
    50821 ctgattgccc gtgcgatcca taatctcagt gggcgtaata atcgccgcat ggtcaaaatg
    50881 aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca tgagcgtggg
    50941 gcttttaccg gtgccagcgc ccagcgtatc ggtcgttttg aactggcgga taaaagctcc
    51001 ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt gctgcgtgta
    51061 ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac ggacgtgcgt
    51121 ctaatcgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgtga gttccgtagc
    51181 gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg cgagcgtccg
    51241 gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc
    51301 aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga gtggccgggt
    51361 aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg cggtaacgtg
    51421 ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc tgccgcaacg
    51481 gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct gaaagaaacc
    51541 aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc
    51601 ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta actgcaaatt
    51661 gccggacaga tctgcctgtc cggcatacta ttcatgaggt tttttcggac gatatttttc
    51721 cggcagttct ggcaccggac gcttgtcatc gatgagatga cgcacggtta agatcggatg
    51781 acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc
    51841 aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca
    51901 tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga
    51961 cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg
    52021 agagatacgc ttgccggaca tgctgcctcc acctcattaa gatgtattta tattacatct
    52081 taatcttaaa gggcactatg actctaaaga agaagggttc gccaaccgac gcaattttgc
    52141 gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc ggaaccagca
    52201 tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc atgtgcgcct
    52261 tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca
    52321 taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtatct
    52381 caagctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg atttttatga
    52441 actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatctcac tgaccaaacg
    52501 cggcgcttca gcgggagagc cgatcccgat ggcggggatc ccctaccatg cggtggaaaa
    52561 ctacctcgcc aaactggtga atcagggcga gtccgttgcc atctgcgaac aaattggcga
    52621 tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta cgccaggcac
    52681 catcagcgat gaagccctgt tgcaggagcg tcaggacaac ctgctggcgg ctatctggca
    52741 ggacagcaaa ggtttcggct acgcgacgct ggatatcagt tccgggcgtt ttcgcctgag
    52801 cgaaccggct gaccgggaaa cgatggcggc agaactgcaa cgcactaatc ctgcggaact
    52861 gctgtatgca gaagattttg ctgaaatgtc gttaattgaa ggccgtcgcg gcctgcgccg
    52921 tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg
    52981 gacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg
    53041 ttgtctgttg cagtatgcga aagataccca acgtacgact ctgccgcata ttcgttccat
    53101 caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacgcgtc gtaatctgga
    53161 aatcacccag aacctggcgg gtggtgcgga aaatacgctg gcttctgtgc tcgactgcac
    53221 cgtcacgccg atgggcagcc gtatgctgaa acgctggctg catatgccag tgcgcgatac
    53281 ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgggct
    53341 acagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc tggctttacg
    53401 aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac tgccggagct
    53461 gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg agaagatggg
    53521 cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac cgccggtgct
    53581 ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg agtggcgcgc
    53641 gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc gtgaacgtac
    53701 cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca ttcaaatcag
    53761 ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc tgaaaaacgc
    53821 cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca cctcaaaagg
    53881 caaagcactg gcactggaaa aacagcttta tgaagagctg ttcgacctgc tgttgccgca
    53941 tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc tggttaacct
    54001 ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata aaccgggcat
    54061 tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc catttatcgc
    54121 taacccgctg aatctgtcgc cgcagcgccg tatgttgatc atcaccggtc cgaacatggg
    54181 cggtaaaagt acctatatgc gccagaccgc gttgattgcg ctgatggcct atatcggcag
    54241 ctacgtaccg gcgcaaaaag tcgagattgg cccgattgac cgtatcttta cccgcgtagg
    54301 cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga ctgaaaccgc
    54361 caatatttta cataacgcca ccgagtacag tctggtgctg atggatgaga ttgggcgcgg
    54421 aacttccact tacgatggtc tgtcgctggc gtgggcgtgc gcggaaaatc tggcgaataa
    54481 gattaaagcg ttgacgctgt ttgccaccca ctatttcgag ctgacacagt taccggagaa
    54541 aatggaaggc gtcgccaacg tgcatctcga tgcactggag cacggcgaca ccattgcctt
    54601 tatacacagc gtgcaggatg gcgcggcgag caaaagctac ggcctggcgg ttgcagctct
    54661 ggccggcgtg ccaaaagagg ttattaagcg cgcacggcaa aaactgcgtg agctggaaag
    54721 catttcgccg aacgccgccg ctacgcaagt ggatggtacg caaatgtctt tgctgtccgt
    54781 accggaagaa acctcgcctg cggtcgaggc actggaaaac ctcgatccgg attcactcac
    54841 cccgcgtcag gcgctgaagt ggatttatcg cctgaagagt ctggtgtaat aataattccc
    54901 gatagtcttt tgctatcggg aatattaacg ataactgacg aatcaaataa aaataccctg
    54961 tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct atcaaaaaat
    55021 caatgcccat cactatcgcc atatatgggt cgttggtgat attcatggtg aatatcagtt
    55081 attacaatcc cgtttacatc aactctcttt ttaccctgaa accgacttac ttatttccac
    55141 aggcgataat attgatcgtg ggccgaagag tcttaacgtg ctgcgcctgc taaaccaacc
    55201 ctggtttacg tcggttaaag gcaaccacga agcgatggcg ttggatgcat tcgaaactgg
    55261 cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa atgattcaga
    55321 gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata ttattgaaat
    55381 cattaatgac aacataaaat atgccatcgc acatgcagat tatccgggtg atgaatatca
    55441 gtttggtaaa gaaatagcag aaagcgaatt actctggcct gttgatcgtg tgcagaaatc
    55501 gcttaatggc gagttacaac aaataaacgg cgctgattat tttatatttg gacatatgat
    55561 gtttgataac attcagacgt tcgctaacca gatttatatt gataccggca cgccgaaaag
    55621 cgggcggctg tcattttata aaataaagta gtctcatgct tcttctgtga agcatgagta
    55681 accaaatgtt attgcaggcc attattcatt tttcgctacc agcaaagaga gatcctgctt
    55741 cgccagcgcg cgactggcac tctccggcaa accgtcgtct gtgataatct gatcaaactc
    55801 gcttaatggt aacgccagcc atgtcgccac ctgaccatat ttcgtcgcat cacagaccaa
    55861 aactcgctgg cggctggcac tggcaatcgc ccgtttcacc gtgactttat cttccgctgg
    55921 cgtagaaatc ccccgcacac tccatgacga tgcagaaata aaagcctgat caatcatcag
    55981 gctgcgcagc atggtcgcag cggcttcccc gacacaggaa cggttttccc gacacactgc
    56041 accgccagtg tgaataattg tgcaattact gttgtcgagc aagtagtctg caataacgaa
    56101 atcgtttgtg accacagtca gtgactccat gtgaatcaga tgctgtgcta tcgctaacgt
    56161 ggtcgttccc gcatccagat agatacaact tcccggctga acaagacttg ccgccagctt
    56221 gccaatagcc gctttttgcg tcattgccag cgcagttttt acctgatgag aaggttcatg
    56281 cgccacgcgt cccggagact ggacgcctcc ggacaccagc acaacggctc cctgctgctc
    56341 cagtttttgt aaatcccgac gaatggtcat atgtgacaca ttcattctgt ccgttagttc
    56401 agcaatactg acaatgcctt tttcagctac catctcaagg atgatttggc gacgctctac
    56461 gggtatcaac ttttgctcct tcctttgtcc tgctgacatt ctacgctatt tgcctgcgaa
    56521 acgtgcgcgg cgcaactaac gcttagttca cataaaataa cacacaatgt taatttatgt
    56581 gaatcagctc accatatcgt tatcttccag cgcttatatt cacaatatca aacaaaatat
    56641 cacttaaatt aacaaggaga gcagatgaaa acgggatctg agtttcatgt cggtatcgtt
    56701 ggcttagggt caatgggaat gggagcagca ctgtcatgtg tccgcgcagg tctttctacc
    56761 tggggcgcag acctgaacag caatgcctgc gctacgttga aagaggcagg tgcttgcggg
    56821 gtttctgata acgccgcgac gtttgccgaa aaactggacg ctctgctggt gctggtggtc
    56881 aatgcggccc aggttaaaca ggtgctgttt ggtgaaacag gcgttgcaca acatctgaaa
    56941 cccggtacgg cagtaatggt ttcttccact atcgctagtg ctgatgcgca agaaattgct
    57001 accgctctgg ctggattcga tctggaaatg ctggatgcgc cagtttctgg tggtgcagta
    57061 aaagccgcta acggtgaaat gactgtcatg gcctccggta gcgatattgc ctttgaacga
    57121 ctggcacccg tgctggaagc cgttgccgga aaagtttatc gcataggtgc agaaccggga
    57181 ctaggttcga ccgtaaaaat tattcaccag ttgttagcgg gcgtacatat tgctgccgga
    57241 gccgaagcga tggcacttgc agcccgtgcg gggatcccgc tggatgtgat gtatgacgtc
    57301 gtgaccaatg ccgccggaaa ttcctggatg ttcgaaaacc ggatgcgtca tgtggtggat
    57361 ggcgattaca ccccgcattc agccgtcgat atttttgtta aggatcttgg tctggttgcc
    57421 gatacagcca aagccctgca cttcccgctg ccattggcct caacagcatt gaatatgttc
    57481 accagcgcca gtaacgcggg ttacgggaaa gaagacgata gcgcagttat caagattttc
    57541 tctggcatca ctctaccggg agcgaaatca tgatcaagat tggcgttatc gccgatgatt
    57601 ttaccggcgc aacggatatc gccagttttc tggtggaaaa cggtctacca acggtacaaa
    57661 ttaacggtgt tccaacaggt aaaatgccgg aagcaatcga cgcactggtg atcagcctga
    57721 aaacgcgttc ctgtccggtg gttgaagcca cacagcaatc gctggcggct ctgagctggt
    57781 tgcaacagca aggttgcaaa cagatctatt tcaaatactg ctctactttc gacagtacgg
    57841 ctaaaggtaa tatcggcccg gttaccgatg cattaatgga tgctctcgac acgccgttta
    57901 cggtcttctc tccggccctg ccggtcaacg gacgtacggt ttatcagggg tatttgttcg
    57961 taatgaatca actgctggcc gaatccggga tgcgccatca cccggtaaat cccatgaccg
    58021 acagctatct tccccgtctg gttgaagcgc aatccacagg gcgctgcggc gtcgtttcgg
    58081 cacatgtttt cgaacaaggt gtggatgccg ttcgtcaaga gctggctcgc ttacagcaag
    58141 agggctaccg ctacgcggtg cttgatgcgc tgaccgaaca ccatctggaa attcagggag
    58201 aagccttgcg cgatgcccca ctggtaacgg gcggttctgg tctggcgatt ggcctggccc
    58261 ggcagtgggc gcaagaaaac ggtaaccagg ctcgcaaagc agggcgtccg ctcgctgggc
    58321 gcggcgtagt gctctccggt tcatgctctc aaatgaccaa ccgccaggta gcacattacc
    58381 gtcaaattgc accagcccgt gaagttgatg tggcacgctg cctctcaact gaaactctgg
    58441 ccacttatgc acacgaactg gcagagtggg ttctgggcca ggaaagtgta cttgctccac
    58501 tggtttttgc caccgccagc actgacgcat tggcagcaat tcaacagcaa tacggtgcac
    58561 aaaaagccag tcaggcagta gaaacactgt tttctcaact agcggcgcgg ttagcggcgg
    58621 aaggcgtggc acgctttatt gtcgcaggcg gtgagacctc cggcgtagtc acacagagcc
    58681 tgggcataaa agggtttcat attggcccaa ccatttcccc cggcgtgccg tgggtaaacg
    58741 cactggataa gcctgtctca ctcgccctta aatctggcaa cttcggtgat gacgcctttt
    58801 tttcacgagc ccaaagagag tttttatcat gagcgatttc gcaaaagtag agcagtcttt
    58861 gcgagaggag atgacgcgga ttgccagttc attctttcag cgcggctatg caaccggttc
    58921 ggctggcaat ctgtcgctgc ttttacctga cgggaattta ctggcgacac cgacaggttc
    58981 atgcctgggc aatctcgatc cgcagcggct ttccaaagtc gccgcggatg gcgaatggtt
    59041 aagtggtgac aaaccctcga aagaggtgct ctttcatctg gcgctgtatc gcaacaatcc
    59101 gcgttgtaaa gcggtggtgc atttgcacag cacatggtcg acggcgcttt cctgcctgca
    59161 agggctggac agcagcaacg ttattcgtcc gttcacacca tacgtggtga tgcggatggg
    59221 aaatgtcccg ctggtgcctt attaccgacc gggcgataaa cgcatcgcac aggatctggc
    59281 ggaactggca gcagacaatc aggctttttt actggcaaat catggcccag tggtttgcgg
    59341 tgaaagcctg caagaagccg ccaacaatat ggaagagctg gaggaaacgg caaagctgat
    59401 ttttattctc ggtgaccgcc cgatccgtta tctgaccgca ggtgaaattg cggaattaag
    59461 gagttaatgc aatgcctcgt tttgcagcta atttatccat gatgttcacc gaagtgcctt
    59521 ttattgaacg cttcgccgca gcgcgaaaag ccgggttcga tgctgtggaa tttctgtttc
    59581 cctatgacta ctccaccctg caaatccaaa agcaactgga gcaaaaccat ctgacactgg
    59641 cgctgttcaa taccgctccc ggagatatta atgcggggga atggggatta tccgcccttc
    59701 ccggacgtga gcacgaagca cacgcagaca tcgacctggc actggaatat gcattggcgc
    59761 tcaattgtga acaagtccat gtgatggcag gcgtcgtgcc cgctggcgaa gacgctgaac
    59821 ggtaccgggc agtatttatc gataatctcc gctacgctgc cgaccgcttt gcgctacacg
    59881 gcaagcgaat attagttgaa gcactcagcc ccgacgttaa gcctcattat ctcttctcca
    59941 gccagtatca ggcactggct attgttgaag aggttgcgcg agataacgtg tttattcaac
    60001 tagatacttt tcatgcgcaa aaagtagatg gcaacctgac gcatttaatc cgcgactacg
    60061 ccgggaaata tgcgcacgta caaattgccg gactacctga tcgacatgaa ccggacgatg
    60121 gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga ggtgggatat cagggctgga
    60181 tcggttgtga atataaacct cgtggcctca ccgaagaagg gcttggctgg tttgacgcct
    60241 ggcgctaatt cgttaaatcc ctaattacaa cgtacccata catcccccca tctacggatg
    60301 gggtaggtta actataactt tcagacaggg tttcccatgt ccacaattac attgttatgc
    60361 attgcgttag ctggcgtaat catgctgttg ctgctggtca tcaaggcaaa ggtacaacca
    60421 ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg cggcgggtat accggcgggc
    60481 gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc ttggttccgt cactattatt
    60541 attggtctgg gcgctatgct ggggcgtatg atcgaacact ctggtggtgc agagtcactg
    60601 gctaattatt tcagtcgcaa gttaggtgac aaacgaacta tcgctgcgct gactctggca
    60661 gcgttcttcc tcggtattcc cgtcttcttt gatgtcggct ttattattct tgcgccaatc
    60721 atttacggtt ttgccaaggt tgccaaaata tcgccactca aatttggcct gcctgtcgct
    60781 gggatcatgc taactgtaca tgtggcggta ccgccgcatc caggccctgt cgccgcagcg
    60841 gggttactcc acgcagacat cggctggcta accatcatcg gtattgcgat ttctattccc
    60901 gtagggattg ttggctactt tgcagcgaaa ataatcaata agcaccaata tgcgatgtcg
    60961 gtggaagtac tggaacagat gcaactggct ccggccagtg aggaaggcgc gacaaaatta
    61021 agcgataaaa taaatccacc gggcgtcgcg ctggtcacct cgctaattgt tattcctatc
    61081 gcgattatca tggcgggtac ggtttccgca acactgatgc cgccttcgca tcccctgctt
    61141 ggtacgctac agctgatcgg ctcaccaatg gtagctctaa tgattgcgct ggtgctggca
    61201 ttctggttat tggctttacg tcgcggctgg agcttacaac acaccagcga cattatgggc
    61261 tcagcccttc ccactgcggc ggtagtgatt ttggttactg gtgctggagg ggtatttggc
    61321 aaagtgctgg tggaatcggg cgtcggcaaa gcccttgcca acatgctaca aatgattgac
    61381 ctgcctctgt taccagccgc atttattatt tcactggcgc tgcgtgcatc gcaggggtca
    61441 gcaaccgtag caatactgac aaccggcggg ttactctcag aagcggtgat gggattgaat
    61501 ccgattcagt gcgtattggt gacgctggca gcctgctttg gtgggcttgg tgcgtcacat
    61561 attaatgact cagggttctg gattgtgacc aaatatctgg ggttgtcggt agcagacggt
    61621 ctgaaaacct ggacagtgtt aacgaccatt ctcggtttta ccggattctt aattacctgg
    61681 tgcgtatggg cggtaatttg accttaataa aaaggtccga tgggcatcgg accttttatt
    61741 gtgcacagaa aaggccagcc tcgcttgaga ctggcctttc tgacagatgc ttacttactc
    61801 gcggaacagc gcttcgatat tcagcccctg cgtttgcagg atttcgcgca aacggcgcag
    61861 gccttcaacc tgaatctggc gaacacgttc acgggtgagg ccaatttcac gacctacatc
    61921 ttccagtgtt gccgcttcgt accccagcaa accgaatcga cgtgccagca cttcacgctg
    61981 tttggcgttc agctcgaaca gccatttgac gatgctctgc ttcatatcgt catcttgcgt
    62041 ggtatcttcc ggaccgttct ctttttcatc ggccaggatg tccagcaacg ctttttcgga
    62101 atcaccaccc agcggggtgt ctaccgaggt aatgcgctcg ttaagacgaa gcatacggct
    62161 gacgtcatca actggcttat ccagttgctc tgcgatctct tccgcacttg gttcatggtc
    62221 cagcttatgg gacaactcac gtgcggttcg caggtaaacg ttcagctcct ttacgatgtg
    62281 aatcggcaaa cgaatagtac gggtttggtt cataatcgcc cgttcaatcg tctggcgaat
    62341 ccaccaggtt gcgtatgttg agaagcggaa accacgttcc gggtcaaact tctctaccgc
    62401 gcggatcagc cccaggttgc cctcttcgat aaggtccagc aacgccagac cacgattgcc
    62461 ataacggcgg gcaattttta ccaccagacg caagttactc tcgatcatcc ggcggcgaga
    62521 ggcgacatct ccacgcagtg cgcgacgcgc aaaataaact tcttcttcgg ccgttaacag
    62581 tggtgaataa ccaatctcac caaggtaaag ctgagtcgcg tccaacacac gctgtgtggc
    62641 tccctgcgat aacagttcct cttcggccaa atcgttatca ctgggttcct cttctactaa
    62701 ggccttttcg tcaaaaacct caactccgtt ctcatcaaat tccgcatctt catttaaatc
    62761 atgaactttc agcgtattct gactcataag gtggctccta cccgtgatcc cttgacggaa
    62821 cattcaagca aaagcctggt tccgccgatt tatcgctgcg gcaaataacg cagcgggttt
    62881 acggatttcc ccttgtaacg aatttcaaaa tgcaagcgtg ttgaactggt tccggtgcta
    62941 cccatggttg ctattttttg ccccgccttc acttcttgtt gttcccggac cagcattgtg
    63001 tcgttatggg cgtaggcact caggtaatca tcattatgtt tgatgataat cagattaccg
    63061 tagccgcgca gcgcgttacc agcataaaca acgcggccat ctgcggtcgc gataattgcc
    63121 tgtcctttgc tgcctgcgat atcaatcccc ttgttgcccc cctcagaagc gccaaaggtt
    63181 tcgatcactt tgccctcagt cggccagcgc caggtggaga taggcgtact ggttgatgta
    63241 ctgctgacag ttggctcggt tgtgcttgct gttggtaccg ttacaggcgc tgtgaccgtg
    63301 gtcgcagctg gcttgttgtt cggcaacatt ttgttagcac tctgttcacc cgaagattca
    63361 gaatacgtaa ttgtcggttg cgacgcaaca gcaacggtgg aattttgtgc aggcttgatc
    63421 acaactcctt gctctgctgc gtcggcctgg gtaatggcat ttccgccagt gattggcgta
    63481 ccggaagcat tacccacctg caaggtctga ccaacgttca gcgcgtatgg tgcctgaata
    63541 ttgttgcgct gagcaaggtc acggaaatcg ttaccagtaa tccaggcgat atagaaaagt
    63601 gtgtcgcctt ttttcacggt ataggtactg ccgctataac tgcctttcgg aatgttccca
    63661 tactgacggt tatagacgat gcgtccgttt tccatctgta ccggctgctg agctactggc
    63721 tgcactggct ggatttgcgg ttgttgagta gcctgaattt gtggctgctg taccggctga
    63781 atttgcggtt gctgggctgt agacgtcgtc cccattttcg gcggcggcgt aatcaacata
    63841 ccagaattgg tatttgcagg cgcattgcca ttaacggagc tgaccggtgc cggtggattt
    63901 gaagtgtcag aacagcctgc cagccatagc gaaaccagtg acaaagccgc aatgcggcga
    63961 acggtgaatt ttgggcttcc cgcgctcatt tatcccccag gaaaaattgg ttaataacca
    64021 gtgacataat taccgtgcaa ggcaccctac tgaacactgg aaaagatgtt cacgatacgc
    64081 tgacctgcgg caaaataacc aggaaaaatc caggtatttc ctcacgtttt aagccagctc
    64141 acccttcact aaagggacaa agcgcacggc ctccacggta tcgataataa attcgcctcc
    64201 ccgacgacgc acccgtttca aatactggtg ctcctcccct acgggtaaga cgagaatccc
    64261 gccttcgtcc agctgcgtca ttagcgcagt tggaatttcc ggcggtgccg ccgtaacaat
    64321 gatagcgtca aacggcgcgc gtgcctgcca accttgccat ccatcgccat gacgggttga
    64381 aacattatgt aaatcaagat ttttcaggcg gcgacgcgcc tgccactgca aacctttaat
    64441 ccgttcgacc gagcaaacat gctggacaag atgcgccagg attgccgttt gatatcccga
    64501 accggtgcca atttccagca cccgcgactg cggcgtcagc tcgagtaatt cggtcattcg
    64561 cgccaccata tacggctgcg aaattgtctg cccctgacct atcggcaagg cgatattgtc
    64621 ccaggctttt tgttcaaacg cttcatcaac gaatttttca cgcggcacgg cggcaagtgc
    64681 attcagcacc tgctcatcct gaataccttg cgcacgtaat tgatccagaa gtgcttgtac
    64741 gcgtctgctt accattgcgt gccaactccc acgctgttta accagtctga aaccacatct
    64801 tgcgcgctat gcgcagttaa atccacatgc agcggcgtga tggagacata gccctcatct
    64861 accgcagcaa aatcggtccc cggaccggca tcacatttac cgcccggcgg gccaatccag
    64921 tacagcgtat tgccgcgcgg atcttgctgc gggatcacct gatctgccgg atgtcgtgta
    64981 ccgcagcgcg tcacgcgaat acctttgatt tgatccaagg gtaaatccgg aacgttaata
    65041 ttaagaatac gcccggtgcg cagcggctct ttacacagtg cgcgcaaaat tgaacaggtt
    65101 accgccgcgg cagtgtcgta atgtttatgc ccgtcaagcg agacggcaag cgccggaaaa
    65161 cctaaatgac ggccttccat cgcggcggct accgtaccgg aataaataac atcatccccc
    65221 agattcggcc cggcgttaat tccggacaca acaatgtccg ggcgcggacg catcagagca
    65281 ttcacgccaa gatagacgca atcggtcggg gttcccattt gcacagcaat atcaccattt
    65341 tcaaaggtaa acgtgcgcag ggaggattcc agtgtcagag aatttgaagc gccgctgcgg
    65401 ttacgatcgg gggcgaccac ctgaacgtca gcaaactcac gcaaggcttt cgccagcgtt
    65461 tgtataccgg gtgcatgtac cccgtcatca ttactcagca atatgcgcat aatcacctgt
    65521 tgtgttgata agttccctga caacgctggt tgcaaaacta cccgccggaa gccagaaacg
    65581 gatctctacg gtgacgtcat cccaccaatt ccagcttaat tgttgcggat acagcagcat
    65641 cgctctgcgc gcggcttcaa ctttttcgcg caccagtaaa gcttgtaatt cagtttctgc
    65701 ggcgacagct gcttgttcga atgccagcgc ttcacgctga gttccccatt cgccactgcc
    65761 tggcaatgcg gcggttatca tcaactcttt atcgttgacg cgacgctgta attccgccag
    65821 ttcttcggtg gttgctacaa accagctacc acgtccggct aattgtagcg catcgccgtc
    65881 aacaacttga ttaacgtctg cttttttgag gcgctcagca acaatctgat taaacaacgc
    65941 actgcgggct gccgacaacc aaaaactccg tttattgcga tcgcgcaccg gagtattggt
    66001 ttgcgcccag cgctgcgccc cctgcaagtt gctaccgcca atcccaaaac gttgggcacc
    66061 gaagtagttc ggtacacctt ttacgcaaat atcgatcaga cgttgttcaa cgtcatcgcg
    66121 attgctcact tcgcgcagaa ccagggtaaa ggcgttacct ttcagcgcgc ctaaacgcag
    66181 cttgcgcttg tgccgcgcat actccagcac ctggcagcct tccagttgaa aggcgctcag
    66241 atcgggcatt tccttgcccg gcacgcgagc gcataaccac tgttccgtaa cagcatgttt
    66301 gtctttttgc ccagcgaagc tgacttcacg ggcatgaatt ttcaggaatt tcgccagtgc
    66361 atccgccaca aaacgggtat tgcagccgtt tttgaggatt ctaaccagaa tatgctcacc
    66421 ttcaccatca ggctcaaagc ccaaatcttc caccaccaca aagtcttccg gattggcttt
    66481 cagcagcccg gtgccttgcg gtttaccgtg gaggtaagtg agattatcaa actcaatcat
    66541 tttgttgcct taatgagtag cgccaccgct tcacaggcaa tcccttcccc acgtccggta
    66601 aatcccagtt tttccgtagt agtggctttc acgttaacat catccatatg gcagccgaga
    66661 tcttcggcaa taaacacgcg catttgtgga atgtgcggca acatcttcgg tgcctgagcg
    66721 atgatagtga catcgacgtt gccaagggta taacccttcg cctgaatacg acgccaggct
    66781 tcgcgtagca gctcgcggct atcggcacct ttaaatgccg gatcggtatc cgggaacagc
    66841 ttgccgatat cccccagcgc cgccgcgcca agcaatgcat cggtcaacgc atggagcgcc
    66901 acgtcgccat cagaatgcgc cagcaatcct ttttcgtaag gaatgcgtac gccaccaatg
    66961 ataattgggc cttcaccgcc aaaggcatgt acgtcaaaac cgtgtccaat tcgcattatg
    67021 tattctcctg atggatggtt cgggtgaggt aaaactcggc cagtgccaaa tcttccgggc
    67081 gcgtgacttt aatgttatcc gcacggcctt cgaccaactg aggatggaat ccgcaatatt
    67141 ccagcgccga ggcttcgtcg gtaatagtcg cgccttcatt tagagcgcgc gtcagacagt
    67201 catgtaacag ctcacgaggg aaaaattgcg gcgtcagcgc gtgccataag ccgttgcgat
    67261 caacggtatg agcaatggca tttttgcccg gttcggcacg tttcatagta tcgcgcactg
    67321 gtgcggcgag gatccccccc gtgcggctgg tttcgctcaa cgccaacaat cgcgcgaggt
    67381 catcctgatg caaacaagga cgagcggcgt catgcaccaa tacccactgc gcgtcgccag
    67441 cggctttcaa acctgccagc acggaatcgg cacgctcatc accgccatct acaacggtga
    67501 tttgcggatg attcgccaga ggaagttgtg caaaacggct atcgccagga cttatggcaa
    67561 tgacgacacg tttcacccgg ggatgcgcca gcagcgcatg caccgagtgt tcaagaatgg
    67621 tttgattacc gattgagaga tattgcttag gacattccgt ttgcattcga cggccaaatc
    67681 cggccgccgg aaccacggcg caaacatcca aatgagtggt tgccatgtta attcccgggc
    67741 tgatttatcg attgttttgc cccgcagact gtgcgcgctt cgacgcgtca ggcaccagac
    67801 gataaaaagt ttcgcccggc ctggtcatgc tgagttcatt acgcgcacgc tcttcgagcg
    67861 cctcctggcc gccattgaga tcgtcaattt cggcaaaaag ttgatcgttt cgcgctttaa
    67921 gtttcgcgtt tgtagcttgc tgtgccgcca catcatcatt gacgcgggta tagtcatgta
    67981 taccgttctt accgaaccac agcgaatact gtagccagac cagaatagcc agcaacagca
    68041 gcgttagttt acccatcctg ccccctgaaa aacggcatca tcatcccatg catccgaaga
    68101 cgactctaca tcctctgttg gggataccgc gacaacgcgg gcaaatgtac cacatttgtc
    68161 cattgttacg tatacccagg gcgtgcagaa cataatctca ttattagtta cggtttgaat
    68221 tatgaacaga ggagacaaga aagtacaaat tagcccagta gccacataaa cagtgcgcca
    68281 aacataatgc ctactgtcac cagggtgaaa acaatactgt agcgtagctt tccgtccatc
    68341 aatgaatgca gcgcaatccc caccactacc gcgacgggca tcagcgccag aaagaaaggc
    68401 caggtgtaga taaagaagaa cagcgtgtta gagccataaa tcaacatcgg catcgccagc
    68461 gcaaataacc aggagataaa accgaccacg gcaccaggca gtgaccatgt ggtttcttca
    68521 tcctcagtaa ggctgtcgtt atttgttagt gtaatgttat ggctattacg catatttgat
    68581 cctgttactt tgacgaaccg ggcatggaaa cccggtggtg tctcaggatc tgataatatc
    68641 gttctgtctc aacagatcta ataattgctg taccaaattt gttactaatt gttcaccatt
    68701 gagatgaatt tctgccgatt caggcgcttc gtaaacggaa tctattcccg taaagttgcg
    68761 cagttcaccg gcacgcgctt tcttatataa gcctttggga tcgcgggctt cgcaaatcgc
    68821 cagcggcgta tcgacaaaca cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat
    68881 ctggcgttcg gcgcggtgtg gcgagataaa tgcggtcagc accaccagtc cggcttcaac
    68941 catcaaattc gccacttcac cgacgcgacg gatattctct ttacgatcgg catcgctaaa
    69001 accgagatcg ctgcataatc cgtggcgaac attgtcgcca tccagcagat acgtactgac
    69061 gccgagttta tgtaacgcct cctccagcgc cccggcgacc gttgatttac cggacccgga
    69121 gaggccggta aaccacagca ctacaccacg atgaccgtgg tgtagctcgc gttgttgcac
    69181 agtgaccgga tggctatgcc agacgacgtt ttcgtcatgc agcgccatta tttatccccc
    69241 agcaaatcgc gcgcgcccca gtgcggaaag tggcgacgaa ccagagcatt caattccagt
    69301 tcgaatgcac tgaattcaga tggcgcagca gttgcctggc taactggctc gtgcaccata
    69361 ccggcaccca cggtcacatt gctcaggcga tcgataaaaa tcagcccacc cgtcaccgga
    69421 ttttgttgat aacgatctaa caccagcggc tcgtcaaaag tgagatccac gaggccgatc
    69481 ccattcagtg gcaggttttc aacttcacgc tgggtaaggt tattaatatc aacctgatag
    69541 cgaatgccat caacacgcgc gcgcgtcttc ttaccggcaa ttttgatgtc gtaactctgc
    69601 cctggagaaa gcggctgttc cgccatccat accacatcca ccgacgcgct ctgcaccgcc
    69661 ggtaacgctt cgtctgccgc cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc
    69721 agcaccaggg tgatcgcttc tccggcaaag gcttcttcgc gatcaccatc aaaagtcacg
    69781 atccgcgcga cgtttgattc cacaccagag ggcagcactt tgacacgttg cccgacttcc
    69841 acgcgaccgg atgccagcgt tccggcgtaa ccacgaaaat cgagattcgg gcgattaacg
    69901 tactgcaccg ggaagcgcat tggctgagca tccaccactc gctggatctc cacggtttcc
    69961 agcacttcga gcagtgtcgg accgctgtac cacggcatac tttcactttg cgatgccacg
    70021 ttgtcgcctt ccagtgcaga gagcggcaca aagcggatat ccagattacc cggcagctgc
    70081 ccggcaaagg tcaaataatc ttcacgaata cgggtgaacg tctcttcact gtaatccacc
    70141 agatccattt tgttgatcgc cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa
    70201 ctgtgacgac gggtttgatc gagcacgcct ttacgggcat cgatcagtaa gatcgccagt
    70261 tcacatgtcg atgcgccagt cgccatattg cgggtgtact gctcgtgccc tggggtgtcg
    70321 gcgataataa atttacgctt ctcggtagag aaatagcggt aggccacgtc aatggtgatg
    70381 ccctgttcgc gctcagcttg caggccgtcc accagcagag ccagatccag cttttcgccc
    70441 tgggtgccgt gacgcttact gtcgttatgc agcgatgaga gctgatcttc gtagatttgg
    70501 cgggtatcgt gcagcagacg accaatcaga gtacttttgc cgtcatcgac gctaccacag
    70561 gtcagaaaac gcagcaggct tttatgttgt tgcgcaatca tccaggcttc gacgccgcct
    70621 tcattggcga tttgttgtgc aagtgcggtg ttcatcttaa aaatacccct gacgtttttt
    70681 cagctccata gaccccgcct ggtcgcggtc aatcacgcgg ccctgacgtt cactggtggt
    70741 ggaaaccagc atctcttcga tgatttccgg cagtgtttgt gcatttgact ccaccgcacc
    70801 ggtcagcggc cagcagccca gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc
    70861 cggttgcagg tcgatacggt tgtcatcaat catcatcaac ataccgtcgc gttccagaac
    70921 cggacgttcc gcagcgagat atagcggaac aatgtcgata ttttccagcc agatgtattg
    70981 ccagatatcc tgctcggtcc agttagagag cgggaagacg cggatgcttt cgcctttgtt
    71041 aatttgcccg ttgtagttgt gccacagctc cgggcgctga tttttcggat cccagcgatg
    71101 gaagcggtca cggaaagagt aaattcgctc tttagcgcgg gatttctctt cgtcacggcg
    71161 cgcaccaccg aaggcggcat caaaaccgta tttgttcagc gcctgtttca ggccttcagt
    71221 tttcataata tcggtatgtt tcgcgctgcc gtgcacgaat ggattaatcc ccatcgccac
    71281 gccttccggg tttttatgca ccagcagttc gcagccgtag gctttagcag tacgatcgcg
    71341 gaactcatac atctcgcgga atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag
    71401 cgtacctgga taaaacgcct tgcgcgccag atgcagcatg acgctggaat ctttaccgat
    71461 agagtagagc atcaccggat ttgagaattc tgccgccacc tcgcgaataa tgtggatgct
    71521 ttccgcctcc agttgccgca ggtgagtaag tcgtatttga tccataaccg ttcctttgca
    71581 ataccgctat tttcttgcca tcagatgttt cgactatagg gagcgtaaga gaacgaatga
    71641 aattaccaat tagaatgagt agttccttaa cggaataacg atttggcaaa gctaatatca
    71701 aaaagtgctt aaggcaccgg atttcgggcg tttaggaaga tttgaaattg ttttagcgca
    71761 gcggcagttt catactatgg cggtaaaaaa atttgcatgg tatttaagga ctcactatgt
    71821 tttccgcatt gcgccaccgt accgctgccc tggcgctcgg cgtatgcttt attctccccg
    71881 tacacgcctc gtcacctaaa cctggcgatt ttgctaatac ccaggcacga catattgcta
    71941 ctttctttcc gggacgcatg accggaactc cagcagaaat gttatctgcc gattatattc
    72001 gccaacagtt tcagcaaatg ggttatcgca gtgatattcg gacatttaat agtcggtata
    72061 tttataccgc ccgcgataat cgcaagaact ggcataacgt gacaggaagt acggtgattg
    72121 ccgctcatga aggcaaagcg ccgcagcaga tcatcattat ggcgcatctg gatacttacg
    72181 ccccgctgag cgatgctgac gccgatgcca atctcggcgg gctgacgtta caaggaatgg
    72241 atgataacgc cgcaggttta ggtgtcatgc tggaattggc agaacgcctg aaaaatacgc
    72301 ctaccgagta tggtattcga tttgtggcga ccagcggcga agaggaaggg aaattaggcg
    72361 ctgagaattt actcaagcgg atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta
    72421 atctcgataa cttaattgtt ggcgataaat tgtatttcaa cagtggtgta aaaacccctg
    72481 aagcagtaag gaaattaacg cgcgacaggg cactggcaat tgcgcgtagt catggaattg
    72541 ccgcaacgac caatccgggt ttgaataaaa attatccgaa gggtactggc tgttgtaatg
    72601 acgccgaagt tttcgacaaa gccggtattg ccgtactttc ggtagaagca acaaattgga
    72661 atcttgggaa taaggatggt tatcagcaac gcgcaaaaac agctgcattc cctgcgggaa
    72721 atagctggca tgacgtaaga ctggataatc aacaacatat tgataaggca cttccaggaa
    72781 gaatagaacg tcgctgccgt gatgttatgc ggataatgct accgctggtg aaggagctgg
    72841 cgaaggcgtc ttgatgggta tgaaaatggg agttgggagt tctaccgcag gggcggggaa
    72901 ctccaagtga tatccatcat cgcatccagt gcgtccggtt tatcctcgct gacgcgggga
    72961 actcttggtt gttcggcgtt ttgaagagaa caaaaacggt ttatccccgc tggcgcgggg
    73021 aactcgactc aatggcaacc gtcacggcct gtcctggcgg tttatccccg ctggcgcggg
    73081 gaactccccc gcttcgctcg agtacccgct ctatatcccg gtttatcccc gctggcgcgg
    73141 ggaactctat ataaacgagg tgaatttatg gaaactgtac ggtttatccc cgctggcgcg
    73201 gggaactctt tttatgcgtg gcagtaaatc cccacgtcgg cggtttatcc ccgctggcgc
    73261 ggggaactcc ggcgaaagcg gatgcgttca ccaatggtca tcggtttatc cccgctggcg
    73321 cggggaactc aaacagattg ttcgttttcc ccatattcat gacggtttat ccccgctggc
    73381 gcggggaact cctttattcc atcccttaat gctttgatga aatcggttta tccccgctgg
    73441 cgcggggaac tcaattgatt gctgggtgat attagaaaac gtcgcggttt atccccgctg
    73501 gcgcggggaa ctcatgtaat gaatattaag cttttcacat tgtcccggtt tatccccgct
    73561 ggcgcgggga actctgttgc agcggcgcac cgctgacgga ctcgcccggt ttatccccgc
    73621 tggcgcgggg aactccggaa ctggtccagc gttaaccggc ggcggatcgg tttatccccg
    73681 ctggcgcggg gaactccggt caggggaaca gcataacaat tccgtgcccg gtttatcccc
    73741 gctggcgcgg ggaactcttg ctgtatagag attatcggcg tgggattgtc ggtttatccc
    73801 cgctggcgcg gggaactctc taaaagtata catttgttct taaagcattt tttcccataa
    73861 aaacaaccca ccaaccttaa tgtaacattt ccttattatt aaagatcagc taattctttg
    73921 ttttcaaaca ggtaaaaaag ataccaacct taaaccatcc aaatctaccg gggtacgcct
    73981 gtttacccca aatgtctgga actcaaatcc cgattccgta ttcgttgccc atgccatcac
    74041 tacattgcct tcttccgcca gtccagctat ttgttcccag atcatttcac gaatttttgc
    74101 ggatacatca cctacatata cccctgcacg tacctccaac aaccagatgg ctaatctgcc
    74161 tcgtaagcgc ggaggtacat tttcagtgac cacgaccaac atactcatct cagctactcc
    74221 gatgtccggc atctcccaga gaaacgggaa gcggaatggc aacaggctgt gcatcttcag
    74281 gtggggccgg cggttgtatt tctccagcag caagcacgtc ctctataagc ggaatcaatt
    74341 tggctaatgt tttactactg cgaaaaatat ccctgcacgc caaacggact tcccggtccg
    74401 gctcaccagg gttacgacgc gctatctcaa aagctttcgg tacaacagtg tcaaatttaa
    74461 tgatgtctgc aatatcgtaa acaaaggaaa gaggctttcc tgtatgcaca aacccaatag
    74521 cgggtgcata accagctgca agtatcgccg cttcagttac gccgtataaa caggaagttg
    74581 cagcgctaat gcattggttg accgtatcgc ccttttccca gtctttcgga tcgtagcgac
    74641 gtccattcca tgtcacgccg tattgcttcg ccagaagtgc gtaggttgcc cgcacgcaac
    74701 tgccttctat acctcggagt tgttctacgg agcgtcgggc gggcgctggt tcgccaaacc
    74761 gtagttcaaa cattttgcgt accactttca gacgcaaatc ttcatccaga gcaagttttg
    74821 cctgatagag cagcttatct gaacgcgcac caccaggctg accagaagca taaacacgaa
    74881 cacccgcttc ccccacccat accaataatg ttccaacttg cgcagccaag cgtacagctg
    74941 catgcgaaac ccgtgtacca ggctccagca tgatgcaggc aaccgagcca acaggaatat
    75001 gagtgcggat ccctgtcttg tcgataagta caaacgcgcc atcgattaca tcgatctgcc
    75061 catattgcag aaagatcatg gagacgcgat ctttgagtgg aatggggtta agaggaagcc
    75121 aggtcatttt attacacctt aatcacagtg gagccaggga tagcaagcca catcccatcg
    75181 atttggctgg cccaatacct tgctgtacaa gatctattaa cgctggcgcg tcgttgatgg
    75241 tgatcacccc ttcaaagcaa accgtttgga tctttccact tttaccatca ccagaaaaat
    75301 actgtggtcg ttccgatatg ggatgcacat cttcaacgcg cgcggcattg cccaatttac
    75361 gctgcaacca cgcgatttgt tctgcttctt ttattaacgg aacccgacag cgttttatat
    75421 tccctttact gtccaggcgt ttttgattgt cgagaatagt ttttatcgga tttgcccgaa
    75481 gccgaaaata gagtggaaca ccaacctgaa gttgaaattc aacctgttta gttttaatga
    75541 ctgtcgcaac ggcagttgaa acaggcattt gcgctgactg caataaaaca tgacagcctt
    75601 ctggtgtgtt tcgcttctca acatgaaaaa gaaaatcacg agcagcatcc ggtctgtttg
    75661 gaaataaatg ccataatccc tggtgaagtt ggtaaagatc cctgctccag gccctggcaa
    75721 tgatgacttt actgagatac atccatacct cctttaatca cataccattc tcgggaagca
    75781 aattgtcgag gcaaggtgat catcggttcg tcgcgcaccg taaattttaa atgatgcccg
    75841 tcaactgatt cctcactata aatatcgccg ccaacgggct cataatttaa tagcaccttc
    75901 tgaggatccg atgcctgaca tgtcccgaga aaaagcggtt gtgttagcgg acaactcctc
    75961 cgccccagat acggtgtata ccgaggcttt aatactgctt tttcaagttc tgacataacc
    76021 atcgttgcct gtggtgttaa ccagagagcg acggtaaagg aggcatcaca taaatattcg
    76081 cgccatgttt gaatcgtttc atgacttttc aaaccacggt aatcttctcg cgctccaagg
    76141 actgtatggt aatcacgcaa ccccgttaca gacacacgac gatcgtcaag aatgagttca
    76201 tcgcagcgca ctgcaaattg cacactctct gataacgcct gtaatgaaga agtatcatca
    76261 cgttggatcc caagacaagc cccgagtagc cctaataacc cgcttcgggt cggaaatctt
    76321 ccggtaggtc gcgttccttc aaaggtcggc tgcccccagg cttgcattgg cccagcaagt
    76381 cgcaagatca aataagatct catgttcacg cctcgccatt attacgaacc caggacttta
    76441 actgttctaa agtaggcatt tgctgtacct ggccagtaat agggtctaca tcagacaagc
    76501 tgaattgcgc agcggctccg ttcagaccat atccattggc aacgcgatcc caatattgat
    76561 taaacgcctg taaagacggt tgcaaaaagc catcgttcgc tttcaccgct ttttcaaaag
    76621 catttgccat agaaagtggc atatcggaga aattaaccat taccatatcc gcagggttaa
    76681 aagcggcata agtacgctgt tttgctccag ggacctctgt tgccagcata tgaacaacat
    76741 gggttgcaat ttccagagcc tgctccctgg aggcaccgcc taaattttcc tgaagttgag
    76801 cgaggttaat attggcataa cgataaaaaa cacccgatga aaattcctgt gttcccagat
    76861 gtgcagaacc ttgttcctgt aaatcatcta cggcggtgaa ccagtcaata tcagaatcaa
    76921 cctgatgggt agtgatcgcg tgcgcaatag acattgcacc atcaactttt cccaactcac
    76981 tcatcatgcc gctggttgcc attcttccac taagcgcaat atcaacaccc tgctgtaaat
    77041 tcacacgtat ggcggcaata tcttccttaa gaactttgag cagcttttta tcatccagat
    77101 tatcagcctc tgcttttgca acctgctcac agaaccaggc tatttccccc acaacccagg
    77161 gagtaaccgc atcggcagaa atcttttcgg cttcatcaac tgatttaccg gagagcagcg
    77221 ctaatgtctt atcgatgatt ttttggtcaa aacgttcact aagtttttgc cgaagaacat
    77281 cacgtaattg tgcaagatga attgttctga gactggattc accaatattt tgtgcgtaat
    77341 aatcactttt acgcatcgca cgtttaaggc tttgacttga aattcttact cgtcttttcc
    77401 cgccgaaaat agcgtctttc tgcatgttca tatcgtcgcg gttcagacat gaagggctgt
    77461 gagagatcag aacatgaata ttgataaagt tagacataga aaggtttcct tacgcatttt
    77521 tgtttgtggt caatacaaaa tcttccagaa gttgctggcg ttcgcgcttt ccccaccagg
    77581 tcaacatcct ggccattaat ggccagtcaa gtacgggttc ggcgtgagca agcaatcgac
    77641 gtaactggac catatcggct gttctgtcag cccgaattaa ttgaaagata cggcgctcgt
    77701 taattcttcc actattggct aaagctcttc ccaacgagat acctgttgtt tgctccggtt
    77761 ttttgtcctg atgtcggatg acattctttc ctgcgctcag gcaaaacacc atgcgtaaaa
    77821 gagcctgctg gtgacgtggg ttttcccaac caaaaggttg caccagccta taaaacgcag
    77881 ggatatcgcg taattcatca ggttctgaaa cacgtctaat ttgcgcacat gctccgttat
    77941 ccagttgttg ccaggctcga tataaagcca ttgcatcaat ttcatcagcc atttgatggc
    78001 cctccttgcg gttttaactc ccgtaaatgt ttgtatagcg tggcgcgggc gagcgctaat
    78061 gtgcttatta atttaggatg atgtgcatag ggcgctacag attgattaaa taacatttca
    78121 cacaattgat gaagtttgtc tcgtaaatca gctatgacct catcagcctg ggaaaaatta
    78181 acattcgcca gtacatcggg aattaataat tcactctgtc gatagaaatg cctttctgca
    78241 gtctcatgaa cagagactcc agccccttta aagtctttat ttttaaaccc ttctgcaaag
    78301 gtatataacg ccttgcgtaa ggctgtttta tatcccaaac caacagtaac tatttcgttt
    78361 atcacattgc cgtattgttg ccacccctga ttaaacatca acacatcatg acgccgttca
    78421 agaatagatg cttgattatt acgatatccc cccataatca attcaagagg actttgcggc
    78481 gcaatatttc tgaattgatt cacaaccgcc gccacgcgat ttccattttc attttgaata
    78541 atcttatcta ccacaactcg gctgatttgt gtccatgatg gtgcggaggt ggtgaaagca
    78601 agaaattttt cctcaacctc ccctttcttg actgttacca gacaagggga atgcggatgg
    78661 ggccatagcc cattaactgt aaaggtaaat ttttccttaa gaaaaccggt ataacgcaaa
    78721 ttgctttcct gtccacagca agaacatttt ccaatcccaa tgggatcgca taattcaata
    78781 tgcgcaggtt gccagaataa gccgcggaca aagccaattg acgaagctgg cacagattca
    78841 ttgggtttga caggcttaac ccaggtaggt tggttttccg tatgtgattc attgggaaat
    78901 tgtttttgaa gacgaggtat tgtgaggaca ttgagtaaca ccgtcgagcg aagatcgatc
    78961 ccacgtacga aggtggttat aggtgttcct ccacgtaaac cgcttttaaa gccaccacca
    79021 aaaccaggtg cctgattcgc ctggttgaat aacgcaatcg cagtgcatcc gccacataat
    79081 gcttcaccct gacccggttg attgacaaaa gcacaattcg tcgcgccgct tacccctgcc
    79141 aacagttttt ccattggagt cacatcattt gctttgacac ctttggtctg cataaaggga
    79201 tgttctgcgt gattaaggta gaacatatct atccacggcg cgatgagttg ttgaaactca
    79261 tcttcagtga gcggattcat tatgcgatgt cgaaattcaa cgtcatcttt tgccgggtcg
    79321 ataatttgcc caatgcaaac cagcagtgct aaagcggcca gttccatatc gtcacggggc
    79381 aaacttaatc gccactgatc tctactgcag tatagcgatt gcagatttat gatttggact
    79441 ttccccccgt ttcgcgggcg tacagggatc cagttatcaa taagcaaatt catttgttct
    79501 ccttcatatg ctccgacatt tctcctgcat ttctatacgt cggcacactt ccgttatcgg
    79561 taaaaacaaa gaaaaaatac ggcgtaatta attcaataat cacattcact gcaaaaatat
    79621 attcattggt taaatacaat taacctatac atatattaag atgtgttgaa ttgtttaaag
    79681 acaataatgc atgcatttca aatctgcaag ttattcgttt tattatcaaa gaaactttta
    79741 gttataataa ttaccataaa ttttattaca taaaatattc atactgcgaa tataaaatct
    79801 cataccggga aattaaaaga agatgtacat tgtgcacctt ccctacttaa gtagggataa
    79861 accgttattg gtcttgttat cgtcattgat accaatcatt cccgaagtta tttgggattt
    79921 gcagggatga ctctggtcat cccttcatcc cgagtgtagg taataacaat actgttaccc
    79981 tgccagaacc atccatcctg attctgtttc ccttccagcc aaagtaaccc atcttcatct
    80041 actacttcag aaaaactacg tttccaggtg aagggtacat tgacgcgatt aagtgcaagc
    80101 gcctcatact gctgttcata acttaggtcc tcgtagacct ggccatcgag cagttgttta
    80161 cctgaagacg tttgtacata aggcaataat ggcaggctca tttccccatc cctcgttacc
    80221 gcaagaatgg tttcatcgtt atcctgcaaa ctatattctt cagcccactg cagaaccttg
    80281 cgagccttga acctcttttc acactcagcg ctttcaaact tatccatgcc tttgataacc
    80341 cattctggct catccatttc agcatcatcg taaatgctat ccagccattg ccggtaggca
    80401 tcagggaaaa ataaggatgc tccattaagt tcctcaatat gttgctgcgt ccgccacatg
    80461 actctaacgt tgctataaat atgctcatgt cgtccgtaac cctcgccatc aggcagcaaa
    80521 atggtggcaa caggaatctc aaaaccagcg ggacgatatt tgcgatgatg gcgatgtaaa
    80581 cgacccaatc gttggaaaag caaatctgcg ggacaatgct gggtaattaa ccaatcaaaa
    80641 tcaacgtcga gcgattgttc cacgacttgc gtggcgacaa gtattcgtcc aacatttcgc
    80701 tccccatttt ttccgaaatc gctaataaca cgattctctt tttctcgacg atcgttcagc
    80761 gtaaagcgtg catgaaacaa atctatatct acttgcgtgt tatttagctc ctttagccgt
    80821 tggtagcata cttgtgcaac gtcaaccaaa ttgcaaataa gacagacttg tgcacccgcg
    80881 tttgccgctg cgatcattcg ctctaacatc gtaaggtcag gtaacatgtc agctaaataa
    80941 ataggttctg gctgaatcga aaagcggggc gggagttgtt ctggatgagc tagcagatca
    81001 aaacgttgcg caccattcac acctcgccag ttaatgagtg gatatgcgga gttattttcc
    81061 actggatctg tatgcagacc ataagtatcc agaagtttct gtttttgttt cattggtagg
    81121 gttgcggaaa gaagaataac actccctccc acatcagcct gagccttgag cactgcctcc
    81181 agcaagccgt tcatataggt gtcgtaagca tgaacttcat caacaattaa aacacttcga
    81241 ccaattccca aaccacggat aaagcggtgt ttaactggca ataccgatat caacacctga
    81301 tcaatcgtgc aaacgccgat ttgcccaaga aacactttct tattgctttg tgacaaccac
    81361 tgacaacact gaacccacgc ttcttcttgc ccctgttcag taatcgcgcg tgattttatt
    81421 gattgaaaga ggtggttaaa ccgtgaattg ccatgagcaa gaataagatt tggggatgaa
    81481 aataagtggc tcgcgctcgc ttccattctc gtaagcatag cattcgcggt agcttgtgtt
    81541 gggagggcaa aaataacact atccgcaatt tgttgatcaa taagtttcca agcataggcc
    81601 agcgctgttt ccgttttacc ggagcctgta ggtgcctcta ttaccgtcag cccgggagct
    81661 actggaagag catcaactaa cacctgtaat tgtctgggtt gatagccatt gtccagtagt
    81721 gcatgaacac cttcataaca tcgcttattt gatacaagtc cactcaactc caatacccgg
    81781 ctcgcatcct gctgtcggtc ctggaaatac gttctcagag catttatgtc ggaaggcgca
    81841 tcctcattaa acagaaaggt attcgttgta gtccaggagc ctaaccagtc agcaagcgag
    81901 caaaaacctg ctaacagtga tgaacaatca ggtggtatat cgtttataga taaccccgct
    81961 ggcgttaaaa ataatgcttc cagtacagat atccactcct cacgagcctg tttatcttgc
    82021 gcagcataag atgccagaga agctggcatt tcccaacgcg acttatcttg atcctgggaa
    82081 tgtaatataa aaccatgatg tcctgtaacg gcctctaccc atggaaacca ggactcataa
    82141 ggatgaggag cggcatcaaa aaaactgaaa aaatccccga gagattgctc tgaaagtgaa
    82201 tcctggttaa accaatacag accggctgca ccatgattaa atttacggca catttgtgtt
    82261 gatggaccat taagtgatgg cgttgcagga tttaatttca gccaactttc tgctgattta
    82321 tattggaatc gtatatcaaa ctttccaata tcatgaagag caatgaaaaa taacagccag
    82381 gccttcaccc tctgttttga tagcatttca tttcggcaaa aagtattttg cagtacgact
    82441 gattgatccc accagcaatc tgcaacagca gcaacatcaa ggcaatgata aattaacaga
    82501 tgaatatcat ttcctttcgt caagcttttt gaggattttc cccagtaatg gcatatatat
    82561 ttaaaaggtt ccattaatag cctccctgtt tttttagtat tatcgatgat atcagcgggc
    82621 tatttttcga tgctgctcat aaatatcgaa ttacagtgat atacacatgt cttgcctccc
    82681 tgcaaagaag agactaacat tcgaattgtt tgggttcgaa cgtaagcctc aggttgatag
    82741 aaatatcgcc tggggctttt gtccatctgg aacctcgcga atgcttaacg ccagacagcc
    82801 tcaagcaccc aacgccattc tatacctgtt aattttccct gcgatgttca cctttaaatg
    82861 ccggaatgct gctcgcaaaa taaaaacgcc cccatcattt ctgacagagg cgtttaattt
    82921 atccggcaat atttaccctt cgtgcaatcc acattccctt tttaagccaa agaaacgtgt
    82981 ttcttcttcc gacatgccag gttcccattt acgggttgta tgggtatcac cgaccgataa
    83041 atatccttca tcccataatg ggtgatattt caggccatgt ttttgcaggt actgataaat
    83101 agttcggtta tcccagtcga taatcggcag cactttaaat acgccacgct gaattgccag
    83161 caccggtaaa ttggcgcggc tgccggattg ttcgcggcgc aggccagcaa accaggtttg
    83221 cgcgttaagt tctttcagcg cccggttcat cggttcgact ttgttgatgt cattgtactt
    83281 ttcaatgcct tcaacgccct gttcccacag tttgccgtag cgtgcttcct gccaggcggc
    83341 gctttcggta gcacggtaca ctttcaggtt gagcttgagt ttgtccgtta actcgtcaat
    83401 aaagcggtag gtttccggaa acagataacc ggtatcggtg aggatcactg gaatatccgg
    83461 ccgaatctga ttcaccagat gcaggctcac cgccgcctga atgccgaagc tggaagaaag
    83521 cacatattca ccgggcagat tatccagcgc ccaggctacg cggccttcag cgtccagttt
    83581 ttccagttgg gcgttagttt ccgccagcgc cagaatgcga tctaccttcg gcagttcgtt
    83641 cagggcgttt agatcgagtt tggacataga ttcctcactg tttgccttgc ctgatgcgac
    83701 gctcacgcgt cttatcaggc ctacaagacc gggctgatgg ttaatcccac aaatcgcgcg
    83761 ccggatcgag caccgggcga atgatgcccg cacgcaccgt aaagtcgccg aagccttcac
    83821 ccgcttcgcg ctctttcgcc cagcgcccta tcagttcatc aagcgacgcc aggatttccg
    83881 gctcggtgat gttttcttta tacatccgag ggatacgtgt cccaatgcgg ttgccgccaa
    83941 gatgcaggtt gtagcgaccc ggcgctttac ccaccaggcc cacttccgcc agcatcgcgc
    84001 gaccacaacc gttcgggcag cctgttacac gcatcacgat atgctcatcg ctgacaccat
    84061 gtttcgccat taaattatcg atgttgtcga taaaagacgg caggaaacgc tccgcttccg
    84121 ccatcgccag cgggcaagtc gggaatgaca cgcaggccat tgagttttca cgctgcggcg
    84181 tgacggcatt cattaacccg ctctctttgg cgatcttctc gatcttcgct ttctcgcttt
    84241 ccggtacacc ggcgatgatc agattctggt tcgccgtaat gcggaaatcg cctttgtgga
    84301 tcttcgcgat ctccagcagg ccggttttca gcggacgcgc cggataatca aggatgcgac
    84361 cattttcgat aaacagcgtc aggtgccagt tatcatcaat gcccttaacc cagccaatac
    84421 gatcgcctcg tccggtgaac tcatatggac ggatcggttc aaatttgatc cccgcgcgac
    84481 gctccacttc cgctttaaac gtctcaaccc ccacgcgctc cagcgtgtat ttggttttgg
    84541 catttttacg atcggttcgg ttaccccagt cacgctgagt tgtcacgacg gcttcggcca
    84601 ccgccagcgt atgctccagc ggcagatagc caaactcact cgcggtgcgg gcgtaggttt
    84661 tcttgttgcc gtgtgcgatg gaaagcccac cgcccaccaa caggttaaag cccaccagct
    84721 tgccgttttc ggcgatcgcc acgaagttca tgtcgttggc gtgcagatcg atatcgttct
    84781 gtggcgggat cactaccgtg gttttgaatt tacgcggcag gtaggtctgg ccgaggatcg
    84841 gttcttcatc agtagtggcg actttttcct ggtcgagcca gatctccgca tacgcgcggg
    84901 tacgcggcag cagatgctca gaaatcttct tcgcccactc gtacgcttcc gcgtgtagct
    84961 gcgactcgtg agggttcgag gtgcagagta cgttacggtt catgtcgtta gcggtcgcca
    85021 gcgcatcaag accaaccgag tgcagcatct ggtgcaccgg tttgacgttc tttttcagaa
    85081 tgccgtggaa ctgaaacgtc tggcggttgg tcaggcgaat gctgccatag atggtgtttt
    85141 caccggcaaa tttgtctatc gcctgccact gtttagtggt aatcacccca cccggcagac
    85201 ggcagcgcaa cagcatcgcg tggcgcggct ccagcttctg ttcagcacgt tcggcgcgga
    85261 tgtcgcggtc atcctgctga tacatgccgt ggaagcggat cagcaggaag ttgtcgccct
    85321 taaagccgcc ggtcagaccg tcgtttaaat cttccgcaat ggtgccgcgc aggtagttgc
    85381 tttcaagctt catgcgctcg gcgtctgtca gttttccttc gaccactaaa ggccctggat
    85441 gtttttcgct cattagtaga catctcgctg ataacggcgc tctacgcgca gctcacttaa
    85501 aaattcatcc gccgcttcgg tgtccatgcc accaaattcg gcaatcactt ccagaagtgc
    85561 ctgctcaacg tctttcgcca tgcgattagc gtcaccgcag acataaatgt gggcaccgtc
    85621 attgatccag cgccacagct ccgcgccctg ttcgcgcagt ttgtcttgta cgtaaacttt
    85681 ttctttttgg tcgcgcgacc aggcgagatc gatccgcgtc agcacaccct ctttgacgta
    85741 gcgctgccac tccacctggt agaggaaatc ttccgtaaag tgcgggttgc caaagaacag
    85801 ccagtttttt cccggcgctt catcggcagc acgttgctgc ataaaggcgc ggaacggcgc
    85861 gatgccggtg cctgggccaa tcataatcac cggggtttct ggattggctg gcaggcggaa
    85921 gttatcgtta tgttcgataa atacgcggac ttcgccctct tcttccacgc ggtcagcgag
    85981 gaagctggag gcaccaccgg cacgggcgcg gccttccacg tcgtaacgca ccacaccaac
    86041 ggtgacgtgt acttcgttct cgacttccgc ctgcgaggag gcaatggaat agagacgcgg
    86101 cgtcagcggg cgcagcagat taattagcgc ttcggcatca agctgtgccg gggagaaacg
    86161 caccatgtca acaatcggcg tcgtcgcggc gtaatgctgt aacttcgctt tatcgcccac
    86221 cagcggcagc agtgtttcac tgcgggtaag cgtggcgtaa ttctcaacaa tgttggcggt
    86281 gttgacggtc agttcgaagt gccactgtag cgcttcgttc agaggcaacg ttttgccctc
    86341 gacggtgaca ggttcatcgc ctttcagcca cagcagttcg acaagttctt tcaccagtgc
    86401 cggatcgttc tgataccaga cgcccagcgc gtcacccggc tggtaacgca ggcccgagtc
    86461 acctaagtca atttcgatat ggcgaacgtc tttttcagag ttacgcccgg taattttctg
    86521 gttaacagag aggctagcca ccagcggcgc gtctttgctg tacgggctgg tgtggatttc
    86581 atttaccgcg ccagtagcga cggattgcga aggtgccgcg acaggcgcac gcgatttaag
    86641 cgcatcaacc acgcgggcgc gccactcgct ggcagcagcc tggtattcaa catcggcatc
    86701 gacacggtcg agcaggcgtt caccacccag ttccgccagc ttgctgtcga aatctttccc
    86761 ggactggcag aaaaattcat aagagctatc gccgaggcta aacacggcaa acgcggtgtt
    86821 ttccagcttt ggcgcttttt tggagaacag gaacttatgc agcgcgacgg cttcttccgg
    86881 cggttcccct tccccttgcg ttgacgtcac tacgatgagc agtttttcgc tggcgatttg
    86941 tttgaattta tagtcgcccg cgttcaccag cttaacgttc agttttgctg ctaataaatc
    87001 atcacgtaat gcttcagcaa cccggcgcgc attgccggtt tgcgaggcgg agataatagt
    87061 tatacccggc atttctgcgg ctggcgctgg cgtcgctgca agcgcagcag gctgctgatt
    87121 gagtacgccc cagaaatagc cagaaaccca ggcaagctgg gtgggagtta aatcggtcgt
    87181 ggccgcctga aggcgtgcca gttgctccgg gttcaacgga agcaacgcgg aaggtgggac
    87241 ctgtgtcgtc atgcgtcgtt atgttccagt aagcaaagct gtttctgcgc cctgtcagcg
    87301 cccataaaac agaagagaag gtaaggttaa cggggcaaac ggtgtggatt aaagacggga
    87361 tagcgataac taataaccaa atcgactaac ctgttttagc aatagtcttt aacaacaaaa
    87421 tagattaacc aacctaatga aaaacaaatg aatttagcca atcattaaga taaatcagcg
    87481 attttgcgca acaaagtcgt tttagataat gcgaaaaaac agcctttccg gtactctacg
    87541 gcggttttat cgtcctgtag agaaattatg atgtccacca cgttatttaa agatttcacc
    87601 ttcgaagccg ctcaccgctt accacacgtc ccggaagggc ataaatgtgg tcgcctgcac
    87661 gggcattcct ttatggtgcg actggaaatt accggggaag tcgatccgca tacgggctgg
    87721 attatcgatt tcgctgaact aaaagcggcg tttaaaccaa cctacgagcg cctcgatcac
    87781 cattatctca atgatattcc aggtctggaa aacccaacca gcgaggtttt agcaaaatgg
    87841 atttgggatc aggttaaacc cgttgtgccg ctgttaagtg cggtgatggt aaaagaaacc
    87901 tgcaccgcag gttgtatcta tcgcggcgaa tgataagagt gtgtcggcgg tcaatttccc
    87961 ttaagtaacg ctatgttagg gtgttgtgtt ctggatatct ggggcatgac atggaagacg
    88021 actgcgacat tattattatt ggtgccggta ttgcaggcac cgcttgcgcg ttacgctgcg
    88081 cgcgagcggg tttatccgtt ttgttactgg aacgcgctga aatccccggc agcaaaaatc
    88141 tttccggcgg gcggttatat acccatgcac tcgcggaact cctcccgcaa tttcatctga
    88201 ccgcgcctct tgaacgacgc atcactcacg aaagcctttc cctgttaacg ccggatggcg
    88261 caacgacgtt ttccagctta cagcccggcg gtgaatcctg gagtgtatta cgtgcacggt
    88321 tcgatccgtg gctggttgcc gaagccgaag aagaaggtgt cgaatgcatc cccggagcga
    88381 cggtggatgc actgtatgaa gaaaacggca gggtgtgtgg tgtcatttgt ggtgacgata
    88441 ttctccgtgc ccgttatgtg gtgctggcag aaggtgccaa cagcgtcctg gctgagcgtc
    88501 acgggttagt gactcgtcct gctggtgaag cgatggcgtt ggggatcaaa gaagtgctgt
    88561 cgctggaacc atccgctatt gaagaacgtt ttcatctgga gaataacgaa ggcgcagcgt
    88621 tgctgttcag cggcaggatc tgtgatgact tacccggcgg cgcatttctt tatactaatc
    88681 aacaaacgct ctcgttaggg attgtttgcc cgctctcttc ccttacgcaa agccgtgttc
    88741 cggcaagcga gctgctgact cgctttaaag cgcatccggc agtgcgcccg cttatcaaaa
    88801 acacggaatc actggagtat ggtgcgcatc tggtgccaga aggtggcttg cacagtatgc
    88861 cggtgcaata cgccggtaac ggctggctgc tggtgggcga tgcgttgcgc agttgcgtca
    88921 ataccggaat ttccgtgcgc ggcatggata tggcgctgac tggcgcgcag gcggcggcac
    88981 aaacgctgat aagcgcctgc cagcaccgcg agccgcaaaa tctgtttccg ctttatcatc
    89041 acaacgtaga gcgcagcctg ctgtgggatg ttctacagcg ttatcagcat gttccggcgc
    89101 ttttgcaacg cccgggatgg taccgtacgt ggcctgcgtt aatgcaagat atttcccgcg
    89161 atttatggga tcagggtgat aaacctgttc caccgctgcg ccagttattc tggcatcatt
    89221 tacgtcgtca cggcctgtgg catctggcgg gcgatgttat caggagtctg cgatgtctgt
    89281 agcccgtaat ctctggcgcg ttgctgatgc gccgcacatt gttccggctg actccgttga
    89341 gcgccagacg gcagaacggt tgattaacgc ctgtccggca ggtctttttt cgctcacacc
    89401 ggaaggtgac ttacgtattg actatcgcag ttgcctggag tgtggcacct gccgtttgct
    89461 gtgcgacgaa tcaacactac aacagtggcg ctatccgcct tccggattcg gcatcaccta
    89521 ccgctttgga taaataataa ggataattta tgcccctctt acacctgctc cgccagaatc
    89581 cggtgattgc tgccgttaaa gataatgcca gcctgcaact ggcaatcgat tctgaatgcc
    89641 aatttatttc cgtgttgtac ggcaatatct gcaccatcag taatatcgtt aaaaagatta
    89701 agaacgccgg gaaatatgct tttattcatg ttgatttgct ggaaggcgcg tcaaataaag
    89761 aagtcgttat tcagtttttg aaactggtga ccgaggcgga cggcattatc agtaccaaag
    89821 cctcaatgtt gaaagcggca agagcggaag gttttttctg tattcatcgc ctgtttattg
    89881 ttgattcaat ttcgtttcac aacattgata agcaagttgc gcaatcgaat ccggattgta
    89941 ttgagatcct gccaggctgt atgcccaaag tgctgggctg ggtgacagag aaaatccgcc
    90001 aaccgctgat tgccggtggg ctggtgtgcg atgaagaaga tgcgcgtaat gcgattaacg
    90061 cgggtgtcgt ggcgctttcc accacgaata ccggggtctg gacgttagcg aaaaaattac
    90121 tttgacggga taatcgtaac caattgaatt tggtttgatt tgtaggccgc acgccacatc
    90181 cgacattcag cgcctgatgc gacgcttgac gcgtcttatc aggcctacaa gtccccaggc
    90241 cagaaccgta ggcaggatca tccggcaaca cgttattggc aatctgcgtg aatgtttgtc
    90301 accagtgcct ccagcaccac tttccagtca tcaaccacgc caacatctgc ctgtgaaaat
    90361 accgcagcgc tggcgtcatg attaatcgcc accacaaact tgctattgcg tacgcccgcc
    90421 attaacgctg ctgcgccaga agcgccaaca acaatacaca cctcaggtgc cagcagatgt
    90481 ccggaaatac caatcacttt ttcagcatca acgccaccgt tcatcactcg cgcccgactg
    90541 tagcccactt cggctcccag cttttctgcc agcatagcga tttcctggtt atctgcctct
    90601 ccgccctgcc ccaccaccag cacccgtctg gcctcagcta atgggtcgcg ggtaacattt
    90661 ttcagatcct ctgtgctaac cagccagtcc ggaagcgcac ccgggacaat gttcagttgt
    90721 tgcattccag agggcaacgt ggcgtttttc gctgctcccg cctgacgcgc cagggaaagg
    90781 catagcgggc gtttttctgt ttgcagcgtc gccgttagcg catttcccca gtgggatttg
    90841 cgcacgctta cggttggaat atctaacgaa gtcacctggc agatgcttgc accatgtaaa
    90901 cgccaggcca gccgggtcga cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg
    90961 tccgcaggcg ttcgctgcca ctgttccacc aacgcatcca gcacctgttc cgccactaca
    91021 ggctgaggtt caatctgcca gtgcgccagg gtacaaccgc tgaaatcctg cgccgccagc
    91081 cagcttgcta ttgcggcatt ctcctgattt attgtcacaa ttgcgatatt catggctgca
    91141 tcctttggcg taaatagtcc tgccatagct tttgagcttt ttctgctacc gtttgcccat
    91201 caatcagcgt cgctccgcgt cgttgctctg ctcttgccag ttgcagacac tgcatcgctg
    91261 gcatttcggc agcaacagtt ttacggataa tttctgcttt ccccgcagcc attcgctggc
    91321 gcattcccgg tactggcaag gccacttcac cgcactgacg cacggcaatc accgcaggca
    91381 agcgaacccg gcaacaacgc aacccatgtt cagtacgctg ttcgagggtg ataaacagcg
    91441 cgtcgagagt gaaacgctcc acctgggtga agcagggcca gcccagcatt tccgccagca
    91501 aaaatggcgt ttgcccattc tgcccttcgc tgctttggca gccggtaatt atcagatcca
    91561 gcggattctg atgctgccat tcggcaatat ggcgagcgac aaattccggc gcaaagcgca
    91621 gatctgccgc cgtctccagc aatacagctt cctcaaaccc aagagccatg agatagcgca
    91681 gccagtgcaa tgcccgttca tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt
    91741 tttttctctg cgccaacagc agcgcggcag cggcctgttc atcagcaccg agtaaacttc
    91801 gcagtagcga aatatccggt ccgcttttac cctgagccgc cgcctgccac tctttttccg
    91861 ccagcattcc ggcatccggt tcggctttaa acgctaacag aatgttcatt cgcacctcct
    91921 acatttcccg ccgccaccag cgggagggct ttagtttctg gtgcccatag ccaggtcaca
    91981 acaaaaccaa ccaacaacac tgtcgccagg agcaataagg tgacttgcat tccccactgc
    92041 gccagcaccc acggcagcag gccagtactt accgccgcgc caagtcgact catggcagtg
    92101 gcaaaaccga cgcccagcga gcgaatgtca gtaggaaaac tttccgcagg caaaatgccc
    92161 accagattac tgactgccga aatggtggtg ctgaagagaa caaaaagcag cagcgttaat
    92221 gaactgccgg aaggcaaaca ggccatgact accagcgttg ccgccagcag caaaaaactt
    92281 cccagcaaaa atttgcgatg tgccagcagg tgcgtcagaa ctaatcccag cagcgcgccc
    92341 acaattaaca acgcattaag catcaggctg gcagtcagcg catcttccag accaatagtc
    92401 tgggcgatag ttggcagcca ggtataaatc acaaaccatg ggattacgag gcagacaaag
    92461 aagacgctgt taaacgccgt gcgccgccag taacgcgaag agaacaaggt tttgatgtgt
    92521 ttatgggtcg ccgttaccac ttcatcgccc agtaaaacat ggggaccaaa atagcgatgc
    92581 acgatagcgt gagcttctgc aaaacgcccc tggcgcagta gccagcgtgg tgattctggc
    92641 gttccccagc gtaataacgt aatcaacaac gcgggcagag ctgccgatgc cagtagccag
    92701 cgccaggcct ccgggttttc ggaaataaag tgatgtccgg caatacttgc cagcacatag
    92761 cctacggtcc acaccacgct gaatgcgccc agcaaaatac cgcgatggcg gcgcggggaa
    92821 aattcagcca gcaaggtgtg acctactgaa taatcgcctc ccagaccaat gccaatcaaa
    92881 atgcgcagtc caataagatg ctctggcgtg gtggcaaaaa attgtaaaaa cgaagcaagc
    92941 gtaatcagca aaaagctgaa ggtgaagatt ttttgccgac caatatggtc ggagatccac
    93001 ccaagaacca ggctgccaag gaacaaacca aggagcgccg agccgccgat cattcccgcc
    93061 ataaacggcg tcagttgcat ggcgggcgta agctgaataa tggcgtaacc aatgacgccg
    93121 agaacataac cgtcggtcag gtgtgcgccg aaagtaagcg cagcaatgcg gcagtgaaaa
    93181 cggttaagcg gtaaatcatc cattcgcacc ggtgaagtgt tcatctttac cccttcattg
    93241 tccggtaata agaacggctt ctcgcctgag aagccgaaaa ggcaacgccc cgtcatcgcg
    93301 gaacgctgcc tgacacatta tttttcaatc ggatagatag tacccgtatt cataatgcca
    93361 ttaggatcga attgcttttt cagcccttcc agcaacgccc acgcgctgcc gtgttccagt
    93421 ttgctccagt gaacgcgatg tttaccgata ccgtggtggt gcaccatcga accgccgagg
    93481 cgaatggttt cttcacagat gatcttgttg agcggattgt ggtacttgtc gatttcctct
    93541 tccggcttac agtcaacgac gttgtaatcg tagacgaagt acatgttggt gccgttctga
    93601 tagctatgag aggaatgacc gcccagcatg gtgatgtcgt cggcatgcgg gaactcagta
    93661 cgaatacggt taataacgct ttcgtagatt tcgtggatgc agctccagca gccggatact
    93721 tcggtggtaa agcccatgtt gccggttttg aggatctgca cacgttcggc agccacttta
    93781 tccggtcccc agttcaggtt gttaaaccag gtttcgatca gcttgctgtc cacgcgctgg
    93841 cattgcgggt agcgggcaac gatttccgca atcccttcgc ccgtcacctt cgcaatgcga
    93901 gggttacctt cagccataaa gatcagcacg cattttccgt cggcaaaatg ggtgaagtgt
    93961 tgggtgccat cttcagcgtc atacaaacga gcgattgacg gacgataccc ttccaccatg
    94021 atttcacgca ggatgttgaa gccggttttc atgtcttcca ggatatagcc gtagaagagg
    94081 ttgttttccg gggtgaattt aaagattttc actgttactt cagtgatata gcacaatgca
    94141 ccttcgttgc cgatgatgat gtgacgaatg tccgggccag ccgcgcggcg tggcacgttt
    94201 ttaatgcgtg tgacggtgcc atcagccaat actgcttcca gaccaacgac catatcttcg
    94261 attgcgccgt agagtgtgga gaactgcccg atacttcggg ttgctaccag gccgcccatc
    94321 tgcgccagcg gctttgactg cggagaatgc cccgtggtgt aacctttttc acgcaacgcg
    94381 ttttccagca cttccagcgg aacaccacat tgcgccgtcg cctgcatatt ctcaatatca
    94441 atattaatga tttgattcat ggcggagccg tcgagcacca ccgagttttc tacaacagtt
    94501 tccagcccac cttcggtggc ggaagcaccg gtacgcggca caccgttaat tttgtgcgca
    94561 ttcataaaat tcagcacacg ggatacttgc tctgtggaac cgagttttac gaccgctgcc
    94621 ggaatcggca aagtataaat gccatgaata tccggaaatt tacgaaaacg gtcaatactg
    94681 tttttcttta atactgtttc atcggtaatt acgcgatctg caccaacaat ttcctttagc
    94741 tggtcgacaa tcgctgcgcg agataaagac atagtaattc cttcctgata ataaaaatgg
    94801 aataatcatt aataagagaa aacagaaacc accgttatgg gttagcgcac taaataaccg
    94861 ccatcaacca ctaataaatg cccgttgaca taattcgatg ccggacttgc gaggaatacg
    94921 gctgcgccca ttaaatcctg agtatcgccc caacggtttg ccggaatatg atcaagaacg
    94981 cgctgattgg tttctggatt actgcgtgtc gccagcgtaa tatctgttgc ataatagcca
    95041 ggggcgatac catttacctg aatattatat tgacctagtt catcacaata agctttggtg
    95101 aacccggcaa gagcatgttt agtggcagaa tatgcaggtg accattgtcc acctaagtaa
    95161 gagaacaatg aacagatatt aataattttg ccgcttttct gcgggatcat aatttttgca
    95221 gcttcatagc ttaactcgaa tgcggcggtc aggttcacat caatcatcgg atcccagtcg
    95281 gcacgaccga agtccagcac cttattcagc ttacaaatac cggcattgtt aaccagaata
    95341 tcaactgtac cgaaacgctc acagcaagca gcgataatct tctgcggcgc gccttctgcg
    95401 gtgatatcca cctgcatgaa gtccacctca acaccctgtt tttcaatcat ttcctttgtt
    95461 tcgccgttat ctttgacgaa actaggaata aagatatttg cgccagcttt ggccaacgcc
    95521 atggcaaatg cctggcctaa accgctattc ccaccggtaa caattgcggt tttacctttc
    95581 agggagaaaa aatccattga gaacgcattg agagattcga ttgacatagt tagctccaat
    95641 ttccgtaagg caaaaaaaaa gaaaggtaac ttcccccgcg agagggaagt tacctttctc
    95701 attatctctg gtaactggag ataattttta gcataagggc ataactgcta atgtgatcgt
    95761 aatcacagtg tgatattcgt attatcaaat aattttaaaa agcagaagcg tgattactct
    95821 cacacaacac ctctgctttc catgttattt attatttcaa ttactagaga cctgagaaaa
    95881 gtaatccacc tgaaagggcg gcttactttt ctcttttttt tgtctattac aaatgggaat
    95941 acgatatgca acacaactca tatcgccgtt ggataaccct cgcgataatt agttttagcg
    96001 gcggcgttag tttcgacctg gcttatttac gttatattta tcaaattccc atggcgaaat
    96061 ttatgggatt cagcaatacc gagataggtt taataatgag tacctttggt attgcggcca
    96121 ttattcttta tgcccccagc ggcgttattg ccgataaatt ttcacaccgc aaaatgatta
    96181 cttccgcgat gatcattacc ggattactgg gtctgttaat ggcaacgtat ccaccgctgt
    96241 gggtaatgct ctgtattcag atcgcctttg cgataacgac gattttaatg ctgtggtcgg
    96301 tgtcgattaa agctgcatcg ttgcttggcg atcatagcga gcaagggaaa attatgggct
    96361 ggatggaagg gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct
    96421 tttctcgctt tgcaccggat gacagcacca gcctgaaaac ggtcattatc atctatagtg
    96481 tggtttacat cttgttgggg attctgtgct ggttttttgt tagcgataac aacaacctgc
    96541 gcagtgccaa taacgaagaa aaacagtcat tccagcttag cgacatcctg gccgttttgc
    96601 gtatcagcac cacctggtat tgcagcatgg tgatttttgg cgtcttcacc atctacgcca
    96661 ttctgagtta ctccaccaac tatctgaccg aaatgtatgg catgtcgctg gtggcggcga
    96721 gctacatggg gattgtgatc aacaaaatat tccgcgcgct gtgcggccca cttggcggca
    96781 taatcaccac ctacagcaaa gtgaaatccc ctacccgcgt gatccaaatc ctttccgtac
    96841 tcggcctgct gacgttaact gccctgctcg tcacgaactc taacccgcaa tcggtcgcga
    96901 tggggattgg cctgatttta ctgctgggat tcacctgtta cgcctcacgc gggctgtact
    96961 gggcctgccc tggcgaagcg agaacaccgt cttacattat gggcaccacg gtaggtattt
    97021 gttcggtgat tggattcctg ccggatgtct tcgtttaccc aattatcggc cactggcaag
    97081 acaccctgcc cgctgcagaa gcctaccgca atatgtggct gatgggcatg gcggcgcttg
    97141 gcatggtgat tgtctttacc tttttgctgt tccaaaaaat tcgtactgct gatagcgccc
    97201 ccgcaatggc tagcagcaag taagccagga aaattacagg agaaaaatca tgtcgaaaaa
    97261 atacatcata gggattgatg gcggaagtca gagcacaaaa gtggtgatgt acgatctgga
    97321 aggtaacgtg gtttgcgaag gcaaaggctt attacagccg atgcacacgc cagatgccga
    97381 tactgcagaa catcctgacg acgatttatg ggcatcatta tgttttgccg gtcacgattt
    97441 gatgagtcag tttgccggga ataaagaaga tattgtcggt attggtctgg gatccatccg
    97501 ttgctgccgt gcgttattga aagccgatgg cacgcctgct gcgccgttga ttagctggca
    97561 ggatgcacgc gttacacgcc cttacgaaca cactaaccct gacgtggcat atgtcacctc
    97621 tttttcgggt tatctgacgc atcgcttaac cggcgagttt aaagacaata tcgccaacta
    97681 ttttggtcag tggccggtgg attataagag ctgggcatgg agcgaagatg ctgcggtaat
    97741 ggataagttt aatatccccc gtcatatgct gtttgatgtg caaatgcctg gcaccgtcct
    97801 cggacatatc acaccacaag ccgcactggc gacacatttc ccggcaggac tgccggttgt
    97861 ttgtaccacc agtgataaac cggtagaagc tctgggggcc ggattactgg atgatgaaac
    97921 ggcggtaatt tctcttggca cttacatcgc attgatgatg aacggcaaag cactgccgaa
    97981 agatccagta gcgtactggc cgattatgtc ttctattccg caaacattgc tgtatgaagg
    98041 ttacggtatt cgcaaaggca tgtggacggt gagctggctg cgcgacatgc taggcgagtc
    98101 gttaattcag gatgccaggg cgcaggatct ttcaccggaa gatttactca acaaaaaagc
    98161 ttcttgtgtg ccgccaggct gtaatggtct gatgacggtg ctggactggc tgaccaatcc
    98221 gtgggaaccg tacaaacgcg ggattatgat cggctttgat tccagcatgg attacgcatg
    98281 gatatatcgt tcgatactgg aaagcgtggc gctgacgctg aagaacaatt acgacaatat
    98341 gtgtaatgaa atgaatcact ttgcgaagca tgtgatcatt actggcggcg gttcgaacag
    98401 cgatctgttt atgcagattt ttgccgacgt gttcaacctt ccggcacgac gtaacgccat
    98461 taacggttgt gcaagcctgg gggcagcgat taatacagcg gtaggtctgg ggctataccc
    98521 ggattacgca acggctgtcg ataacatggt tcgcgtgaaa gatatcttta taccgattga
    98581 gagcaatgcc aaacgctacg acgcgatgaa taaaggcatt ttcaaagacc taaccaaaca
    98641 tactgatgtg atcctgaaaa aatcgtatga agtgatgcat ggggaattgg ggaatgtgga
    98701 ttcgatccag agctggtcga atgcgtaagt gaggaaggcc gggcgggaaa ctgcccggcc
    98761 tgaacatacc tgaatggtta tccccgctga cgcggggaac ataagttcga caacttaaaa
    98821 aatgataaca agtccggttt atccccgctg atgcagggaa cataatataa actttaacta
    98881 gttcggttta tccctgtttt tacaaggaat attaactaca ctcgatgtct ttagaataaa
    98941 agacaatacg aatcgcactt tatcaatttt cacttaaagt atgaactgga tatctttatt
    99001 cataagaact attcatcaac agcgtataga ggcggttatg taaaaccact cattagcctc
    99061 aaaacaaaga caaacttcca gatttattac ttaaccaatg aataacaagc ttaaaaaata
    99121 tactgttatt ctaaagaaaa aattaacagg cattaattca atattaaaaa aataatagat
    99181 taaaatttct taacgattta agaatcatac aaataacact ttgattaaat ttaatttttt
    99241 gtatcgataa ttgtgaacct ctctggcatg gagaactatt ttgaacatga ggtgttacgt
    99301 ggatatgttg cttattacaa gtactgctaa tataaaaact tgagaaagag ataacgggtt
    99361 atatggtggt ttatccccgc tggcgcgggg aactcgacag aacggcctca gtagtctcgt
    99421 caggctccgg tttatccccg ctggcgcggg gaacacctgt tttcgcaaat ctatggacta
    99481 ttgctattcg gtttatcccc gctggcgcgg ggaacacggg cgcacggaat acaaagccgt
    99541 gtatctgctc ggtttatccc cgctggcgcg gggaacactg gctctgcaac agcagcaccc
    99601 atgaccacgt cggtttatcc ccgctggcgc ggggaacacg aaatgctggt gagcgttaat
    99661 gccgcaaaca caggtttatc cccgctggcg cggggaacac attacgcctt tttgcgattg
    99721 cccggttttt gccggtttat ccccgctggc gcggggaaca ctttgccacc cgagtccata
    99781 aattcttgat atgcggttta tccccgctgg cgcggggaac accctcatgt ggattatatt
    99841 aatattatgg gccgcg
//
LOCUS       DAZFZC010000003        97520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFZC010000003 DAZFZC010000000
VERSION     DAZFZC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 97520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.003"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..1808
                     /gene="adeD"
                     /locus_tag="ACHKXP_000216"
     CDS             42..1808
                     /gene="adeD"
                     /locus_tag="ACHKXP_000216"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO7645295.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1854..3245)
                     /gene="uhpT"
                     /locus_tag="ACHKXP_000217"
     CDS             complement(1854..3245)
                     /gene="uhpT"
                     /locus_tag="ACHKXP_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO7645296.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3383..4702)
                     /gene="uhpC"
                     /locus_tag="ACHKXP_000218"
     CDS             complement(3383..4702)
                     /gene="uhpC"
                     /locus_tag="ACHKXP_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO7645297.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4712..6214)
                     /gene="uhpB"
                     /locus_tag="ACHKXP_000219"
     CDS             complement(4712..6214)
                     /gene="uhpB"
                     /locus_tag="ACHKXP_000219"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO7645298.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6214..6804)
                     /gene="uhpA"
                     /locus_tag="ACHKXP_000220"
     CDS             complement(6214..6804)
                     /gene="uhpA"
                     /locus_tag="ACHKXP_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO7645299.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6880..7170)
                     /gene="ilvN"
                     /locus_tag="ACHKXP_000221"
     CDS             complement(6880..7170)
                     /gene="ilvN"
                     /locus_tag="ACHKXP_000221"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7645300.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7174..8862)
                     /gene="ilvB"
                     /locus_tag="ACHKXP_000222"
     CDS             complement(7174..8862)
                     /gene="ilvB"
                     /locus_tag="ACHKXP_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO7645301.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQASLQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8968..9066)
                     /gene="ivbL"
                     /locus_tag="ACHKXP_000223"
     CDS             complement(8968..9066)
                     /gene="ivbL"
                     /locus_tag="ACHKXP_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO7645302.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9631..9720
                     /gene="tisB"
                     /locus_tag="ACHKXP_000224"
     CDS             9631..9720
                     /gene="tisB"
                     /locus_tag="ACHKXP_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO7645303.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9844..9918)
                     /gene="ysdE"
                     /locus_tag="ACHKXP_000225"
     CDS             complement(9844..9918)
                     /gene="ysdE"
                     /locus_tag="ACHKXP_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO7645304.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10000..11184
                     /gene="emrD"
                     /locus_tag="ACHKXP_000226"
     CDS             10000..11184
                     /gene="emrD"
                     /locus_tag="ACHKXP_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO7645305.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11192..11689)
                     /gene="yidF"
                     /locus_tag="ACHKXP_000227"
     CDS             complement(11192..11689)
                     /gene="yidF"
                     /locus_tag="ACHKXP_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO7645306.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11686..12048)
                     /gene="yidG"
                     /locus_tag="ACHKXP_000228"
     CDS             complement(11686..12048)
                     /gene="yidG"
                     /locus_tag="ACHKXP_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO7645307.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12038..12385)
                     /gene="yidH"
                     /locus_tag="ACHKXP_000229"
     CDS             complement(12038..12385)
                     /gene="yidH"
                     /locus_tag="ACHKXP_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO7645308.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12493..12942
                     /gene="yidI"
                     /locus_tag="ACHKXP_000230"
     CDS             12493..12942
                     /gene="yidI"
                     /locus_tag="ACHKXP_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO7645309.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILNQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12989..14482)
                     /gene="yidJ"
                     /locus_tag="ACHKXP_000231"
     CDS             complement(12989..14482)
                     /gene="yidJ"
                     /locus_tag="ACHKXP_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7645310.1"
                     /translation="MKRPNFLFVMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14479..16194)
                     /gene="yidK"
                     /locus_tag="ACHKXP_000232"
     CDS             complement(14479..16194)
                     /gene="yidK"
                     /locus_tag="ACHKXP_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO7645311.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     GKDSL"
     gene            16361..17254
                     /gene="yidL"
                     /locus_tag="ACHKXP_000233"
     CDS             16361..17254
                     /gene="yidL"
                     /locus_tag="ACHKXP_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7645312.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17251..18065)
                     /locus_tag="ACHKXP_000234"
                     /pseudo
     CDS             complement(17251..18065)
                     /locus_tag="ACHKXP_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18065..19681)
                     /locus_tag="ACHKXP_000235"
     CDS             complement(18065..19681)
                     /locus_tag="ACHKXP_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7645313.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19977..20693
                     /gene="yidP"
                     /locus_tag="ACHKXP_000236"
     CDS             19977..20693
                     /gene="yidP"
                     /locus_tag="ACHKXP_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7645314.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLQRLHPEKS"
     gene            complement(20690..22351)
                     /gene="yidE"
                     /locus_tag="ACHKXP_000237"
     CDS             complement(20690..22351)
                     /gene="yidE"
                     /locus_tag="ACHKXP_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO7645315.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22547..22975)
                     /gene="ibpB"
                     /locus_tag="ACHKXP_000238"
     CDS             complement(22547..22975)
                     /gene="ibpB"
                     /locus_tag="ACHKXP_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO7645316.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23087..23500)
                     /gene="ibpA"
                     /locus_tag="ACHKXP_000239"
     CDS             complement(23087..23500)
                     /gene="ibpA"
                     /locus_tag="ACHKXP_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO7645317.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23494..23566)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23806..24138
                     /gene="yidQ"
                     /locus_tag="ACHKXP_000240"
     CDS             23806..24138
                     /gene="yidQ"
                     /locus_tag="ACHKXP_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7645318.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24140..25354)
                     /gene="yidR"
                     /locus_tag="ACHKXP_000241"
     CDS             complement(24140..25354)
                     /gene="yidR"
                     /locus_tag="ACHKXP_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO7645319.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGESIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIVE
                     GGKMSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHTLAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25455..26519
                     /gene="cbrA"
                     /locus_tag="ACHKXP_000242"
     CDS             25455..26519
                     /gene="cbrA"
                     /locus_tag="ACHKXP_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO7645320.1"
                     /translation="MEHFDVAIIGIGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(26516..27853)
                     /gene="dgoT"
                     /locus_tag="ACHKXP_000243"
     CDS             complement(26516..27853)
                     /gene="dgoT"
                     /locus_tag="ACHKXP_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7645321.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27928..29076)
                     /gene="dgoD"
                     /locus_tag="ACHKXP_000244"
     CDS             complement(27928..29076)
                     /gene="dgoD"
                     /locus_tag="ACHKXP_000244"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO7645322.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29073..29690)
                     /gene="dgoA"
                     /locus_tag="ACHKXP_000245"
     CDS             complement(29073..29690)
                     /gene="dgoA"
                     /locus_tag="ACHKXP_000245"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO7645323.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29674..30552)
                     /gene="dgoK"
                     /locus_tag="ACHKXP_000246"
     CDS             complement(29674..30552)
                     /gene="dgoK"
                     /locus_tag="ACHKXP_000246"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO7645324.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREEKTPVVMAGMVGSNVGWKVAPYLSVPACFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30549..31238)
                     /gene="dgoR"
                     /locus_tag="ACHKXP_000247"
     CDS             complement(30549..31238)
                     /gene="dgoR"
                     /locus_tag="ACHKXP_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO7645325.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31516..32172
                     /gene="yidX"
                     /locus_tag="ACHKXP_000248"
     CDS             31516..32172
                     /gene="yidX"
                     /locus_tag="ACHKXP_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO7645326.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32218..33030)
                     /gene="yidA"
                     /locus_tag="ACHKXP_000249"
     CDS             complement(32218..33030)
                     /gene="yidA"
                     /locus_tag="ACHKXP_000249"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO7645327.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33145..33543)
                     /gene="yidB"
                     /locus_tag="ACHKXP_000250"
     CDS             complement(33145..33543)
                     /gene="yidB"
                     /locus_tag="ACHKXP_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO7645328.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33783..36197)
                     /gene="gyrB"
                     /locus_tag="ACHKXP_000251"
     CDS             complement(33783..36197)
                     /gene="gyrB"
                     /locus_tag="ACHKXP_000251"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO7645329.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36226..37299)
                     /gene="recF"
                     /locus_tag="ACHKXP_000252"
     CDS             complement(36226..37299)
                     /gene="recF"
                     /locus_tag="ACHKXP_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO7645330.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37299..38399)
                     /gene="dnaN"
                     /locus_tag="ACHKXP_000253"
     CDS             complement(37299..38399)
                     /gene="dnaN"
                     /locus_tag="ACHKXP_000253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO7645331.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38404..39807)
                     /gene="dnaA"
                     /locus_tag="ACHKXP_000254"
     CDS             complement(38404..39807)
                     /gene="dnaA"
                     /locus_tag="ACHKXP_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO7645332.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQVELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40104..40184
                     /locus_tag="ACHKXP_000255"
     CDS             40104..40184
                     /locus_tag="ACHKXP_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645333.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40414..40554
                     /gene="rpmH"
                     /locus_tag="ACHKXP_000256"
     CDS             40414..40554
                     /gene="rpmH"
                     /locus_tag="ACHKXP_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO7645334.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40571..40930
                     /gene="rnpA"
                     /locus_tag="ACHKXP_000257"
     CDS             40571..40930
                     /gene="rnpA"
                     /locus_tag="ACHKXP_000257"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO7645335.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40894..41151
                     /gene="yidD"
                     /locus_tag="ACHKXP_000258"
     CDS             40894..41151
                     /gene="yidD"
                     /locus_tag="ACHKXP_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO7645336.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41154..42800
                     /gene="yidC"
                     /locus_tag="ACHKXP_000259"
     CDS             41154..42800
                     /gene="yidC"
                     /locus_tag="ACHKXP_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO7645337.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42906..44270
                     /gene="mnmE"
                     /locus_tag="ACHKXP_000260"
     CDS             42906..44270
                     /gene="mnmE"
                     /locus_tag="ACHKXP_000260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO7645338.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44512..44586
                     /gene="tnaC"
                     /locus_tag="ACHKXP_000261"
     CDS             44512..44586
                     /gene="tnaC"
                     /locus_tag="ACHKXP_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO7645339.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44807..46222
                     /gene="tnaA"
                     /locus_tag="ACHKXP_000262"
     CDS             44807..46222
                     /gene="tnaA"
                     /locus_tag="ACHKXP_000262"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO7645340.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46313..47560
                     /gene="tnaB"
                     /locus_tag="ACHKXP_000263"
     CDS             46313..47560
                     /gene="tnaB"
                     /locus_tag="ACHKXP_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO7645341.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWLSSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47692..48867
                     /gene="mdtL"
                     /locus_tag="ACHKXP_000264"
     CDS             47692..48867
                     /gene="mdtL"
                     /locus_tag="ACHKXP_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO7645342.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48842..49801
                     /gene="yidZ"
                     /locus_tag="ACHKXP_000265"
     CDS             48842..49801
                     /gene="yidZ"
                     /locus_tag="ACHKXP_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO7645343.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRINVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            49958..50707
                     /gene="yieE"
                     /locus_tag="ACHKXP_000266"
     CDS             49958..50707
                     /gene="yieE"
                     /locus_tag="ACHKXP_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO7645344.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            50729..51295
                     /gene="yieF"
                     /locus_tag="ACHKXP_000267"
                     /gene_synonym="chrR"
     CDS             50729..51295
                     /gene="yieF"
                     /locus_tag="ACHKXP_000267"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO7645345.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51349..52686)
                     /gene="adeP"
                     /locus_tag="ACHKXP_000268"
     CDS             complement(51349..52686)
                     /gene="adeP"
                     /locus_tag="ACHKXP_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO7645346.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            52851..53516
                     /gene="yieH"
                     /locus_tag="ACHKXP_000269"
     CDS             52851..53516
                     /gene="yieH"
                     /locus_tag="ACHKXP_000269"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO7645347.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            53583..54056
                     /gene="cbrB"
                     /locus_tag="ACHKXP_000270"
     CDS             53583..54056
                     /gene="cbrB"
                     /locus_tag="ACHKXP_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO7645348.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            54105..54692
                     /gene="cbrC"
                     /locus_tag="ACHKXP_000271"
     CDS             54105..54692
                     /gene="cbrC"
                     /locus_tag="ACHKXP_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO7645349.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(54754..55476)
                     /gene="yieK"
                     /locus_tag="ACHKXP_000272"
     CDS             complement(54754..55476)
                     /gene="yieK"
                     /locus_tag="ACHKXP_000272"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7645350.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(55491..56660)
                     /gene="yieL"
                     /locus_tag="ACHKXP_000273"
     CDS             complement(55491..56660)
                     /gene="yieL"
                     /locus_tag="ACHKXP_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7645351.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDANGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(56687..58303)
                     /gene="bglH"
                     /locus_tag="ACHKXP_000274"
     CDS             complement(56687..58303)
                     /gene="bglH"
                     /locus_tag="ACHKXP_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO7645352.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(58389..59783)
                     /gene="bglB"
                     /locus_tag="ACHKXP_000275"
     CDS             complement(58389..59783)
                     /gene="bglB"
                     /locus_tag="ACHKXP_000275"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO7645353.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(59802..61679)
                     /gene="bglF"
                     /locus_tag="ACHKXP_000276"
     CDS             complement(59802..61679)
                     /gene="bglF"
                     /locus_tag="ACHKXP_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO7645354.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(61812..62648)
                     /gene="bglG"
                     /locus_tag="ACHKXP_000277"
     CDS             complement(61812..62648)
                     /gene="bglG"
                     /locus_tag="ACHKXP_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO7645355.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(62934..63659)
                     /gene="phoU"
                     /locus_tag="ACHKXP_000278"
     CDS             complement(62934..63659)
                     /gene="phoU"
                     /locus_tag="ACHKXP_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO7645356.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(63674..64447)
                     /gene="pstB"
                     /locus_tag="ACHKXP_000279"
     CDS             complement(63674..64447)
                     /gene="pstB"
                     /locus_tag="ACHKXP_000279"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO7645357.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(64630..65520)
                     /gene="pstA"
                     /locus_tag="ACHKXP_000280"
     CDS             complement(64630..65520)
                     /gene="pstA"
                     /locus_tag="ACHKXP_000280"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO7645358.1"
                     /translation="MAMVEMQTNAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(65520..66479)
                     /gene="pstC"
                     /locus_tag="ACHKXP_000281"
     CDS             complement(65520..66479)
                     /gene="pstC"
                     /locus_tag="ACHKXP_000281"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO7645359.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(66565..67605)
                     /gene="pstS"
                     /locus_tag="ACHKXP_000282"
     CDS             complement(66565..67605)
                     /gene="pstS"
                     /locus_tag="ACHKXP_000282"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO7645360.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(67919..69748)
                     /gene="glmS"
                     /locus_tag="ACHKXP_000283"
     CDS             complement(67919..69748)
                     /gene="glmS"
                     /locus_tag="ACHKXP_000283"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO7645361.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(69910..71280)
                     /gene="glmU"
                     /locus_tag="ACHKXP_000284"
     CDS             complement(69910..71280)
                     /gene="glmU"
                     /locus_tag="ACHKXP_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO7645362.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(71633..72052)
                     /gene="atpC"
                     /locus_tag="ACHKXP_000285"
     CDS             complement(71633..72052)
                     /gene="atpC"
                     /locus_tag="ACHKXP_000285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO7645363.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(72073..73455)
                     /gene="atpD"
                     /locus_tag="ACHKXP_000286"
     CDS             complement(72073..73455)
                     /gene="atpD"
                     /locus_tag="ACHKXP_000286"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO7645364.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(73482..74345)
                     /gene="atpG"
                     /locus_tag="ACHKXP_000287"
     CDS             complement(73482..74345)
                     /gene="atpG"
                     /locus_tag="ACHKXP_000287"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO7645365.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(74396..75937)
                     /gene="atpA"
                     /locus_tag="ACHKXP_000288"
     CDS             complement(74396..75937)
                     /gene="atpA"
                     /locus_tag="ACHKXP_000288"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO7645366.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(75950..76483)
                     /gene="atpH"
                     /locus_tag="ACHKXP_000289"
     CDS             complement(75950..76483)
                     /gene="atpH"
                     /locus_tag="ACHKXP_000289"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO7645367.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(76498..76968)
                     /gene="atpF"
                     /locus_tag="ACHKXP_000290"
     CDS             complement(76498..76968)
                     /gene="atpF"
                     /locus_tag="ACHKXP_000290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO7645368.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(77030..77269)
                     /gene="atpE"
                     /locus_tag="ACHKXP_000291"
     CDS             complement(77030..77269)
                     /gene="atpE"
                     /locus_tag="ACHKXP_000291"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO7645369.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(77316..78131)
                     /gene="atpB"
                     /locus_tag="ACHKXP_000292"
     CDS             complement(77316..78131)
                     /gene="atpB"
                     /locus_tag="ACHKXP_000292"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO7645370.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(78140..78520)
                     /gene="atpI"
                     /locus_tag="ACHKXP_000293"
     CDS             complement(78140..78520)
                     /gene="atpI"
                     /locus_tag="ACHKXP_000293"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO7645371.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(79137..79760)
                     /gene="rsmG"
                     /locus_tag="ACHKXP_000294"
     CDS             complement(79137..79760)
                     /gene="rsmG"
                     /locus_tag="ACHKXP_000294"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO7645372.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(79824..81713)
                     /gene="mnmG"
                     /locus_tag="ACHKXP_000295"
     CDS             complement(79824..81713)
                     /gene="mnmG"
                     /locus_tag="ACHKXP_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO7645373.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(82092..82535)
                     /gene="mioC"
                     /locus_tag="ACHKXP_000296"
     CDS             complement(82092..82535)
                     /gene="mioC"
                     /locus_tag="ACHKXP_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO7645374.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(82625..83083)
                     /gene="asnC"
                     /locus_tag="ACHKXP_000297"
     CDS             complement(82625..83083)
                     /gene="asnC"
                     /locus_tag="ACHKXP_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO7645375.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            83235..84227
                     /gene="asnA"
                     /locus_tag="ACHKXP_000298"
     CDS             83235..84227
                     /gene="asnA"
                     /locus_tag="ACHKXP_000298"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO7645376.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(84232..85683)
                     /gene="viaA"
                     /locus_tag="ACHKXP_000299"
     CDS             complement(84232..85683)
                     /gene="viaA"
                     /locus_tag="ACHKXP_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO7645377.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSAITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(85677..87173)
                     /gene="ravA"
                     /locus_tag="ACHKXP_000300"
     CDS             complement(85677..87173)
                     /gene="ravA"
                     /locus_tag="ACHKXP_000300"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO7645378.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            87396..89264
                     /gene="kup"
                     /locus_tag="ACHKXP_000301"
     CDS             87396..89264
                     /gene="kup"
                     /locus_tag="ACHKXP_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO7645379.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            89431..89850
                     /gene="rbsD"
                     /locus_tag="ACHKXP_000302"
     CDS             89431..89850
                     /gene="rbsD"
                     /locus_tag="ACHKXP_000302"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO7645380.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            89858..91363
                     /gene="rbsA"
                     /locus_tag="ACHKXP_000303"
     CDS             89858..91363
                     /gene="rbsA"
                     /locus_tag="ACHKXP_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO7645381.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            91368..92333
                     /gene="rbsC"
                     /locus_tag="ACHKXP_000304"
     CDS             91368..92333
                     /gene="rbsC"
                     /locus_tag="ACHKXP_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO7645382.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            92358..93248
                     /gene="rbsB"
                     /locus_tag="ACHKXP_000305"
     CDS             92358..93248
                     /gene="rbsB"
                     /locus_tag="ACHKXP_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO7645383.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            93374..94303
                     /gene="rbsK"
                     /locus_tag="ACHKXP_000306"
     CDS             93374..94303
                     /gene="rbsK"
                     /locus_tag="ACHKXP_000306"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7645384.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            94307..95299
                     /gene="rbsR"
                     /locus_tag="ACHKXP_000307"
     CDS             94307..95299
                     /gene="rbsR"
                     /locus_tag="ACHKXP_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO7645385.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(95265..96692)
                     /gene="mdtD"
                     /locus_tag="ACHKXP_000308"
     CDS             complement(95265..96692)
                     /gene="mdtD"
                     /locus_tag="ACHKXP_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO7645386.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(96715..97407)
                     /gene="yieP"
                     /locus_tag="ACHKXP_000309"
     CDS             complement(96715..97407)
                     /gene="yieP"
                     /locus_tag="ACHKXP_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO7645387.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT        23776 a        25588 c        24575 g        23581 t
ORIGIN      
        1 ataaataata atcaaattga taaaatcaaa atgagaaaaa tatgaataat tctattaacc
       61 ataaatttca tcacattagc cgggctgaat accaggaatt gttagccgtt tcccgtggcg
      121 acgctgttgc cgattatatt attgataatg tctctattct cgacctgatc aatggcggag
      181 aaatttccgg cccaattgtg attaaaggac gttacattgc cggtgttggc gcagaataca
      241 ctgatgctcc ggctttgcag cggattgatg ctcgcggcgc aacggcggtg ccagggttta
      301 ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcactttt gaaaccgcta
      361 ccctgccgcg cggcctgacg accgttattt gcgaccctca tgaaatcgtc aacgtgatgg
      421 gcgaagccgg attcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa aaccagtact
      481 tacaggtcag ctcttgcgta cccgccctgg aaggctgcga tgttaacggt gccagtttta
      541 cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt gcagaaatga
      601 tggactaccc tggcgtaatt agcgggcaga atgcgctgct cgataaactg gatgcatttc
      661 gccacctgac gctggacggt cactgcccgg gtttgggtgg taaagaactt aacgcctata
      721 ttactgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga cgccggaaat
      781 tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgcccgcaat ctcaacgcgc
      841 tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtacc gatgaccgta
      901 acccgtggga gatcgcccat gaaggacaca tcgatgcctt aattcgccgc ctgatcgaac
      961 aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg gcgcgccact
     1021 ttggtctgaa tcatctcggc ttactggcac ccggcaagca ggccgatatc gtcctgttga
     1081 gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgagccg attgatgcgc
     1141 aaaccttaca ggcggaagag tcggcgagac tggcacaatc cgctccgcca tatggcaaca
     1201 ccattgcccg ccagccagtt tccgccagcg actttgccct gcaatttacg cccggaaaac
     1261 gctatcgggt cattgacgtc atccataacg aattgattac gcactcccac tccagcgtct
     1321 acagcgaaaa tggttttgat cgcgatgatg tgagctttat tgccgtactt gagcgttacg
     1381 ggcaacggct ggctccggct tgtggtttgc ttggcggctt tggactgaat gaaggtgcgc
     1441 tggctgcgac ggtcagccat gacagccata atattgtggt gatcggtcgc agtgccgaag
     1501 agatggcgct ggcggtcaat caggtgattc aggatggcgg cgggctgtgc gtggtacgta
     1561 acggccaggt acaaagtcat ctgccgttac ccattgccgg gctgatgagc accgacacgg
     1621 cgcagtcgct ggcggaacaa attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt
     1681 tacccgatga gccgtttatt cagatggcgt ttctttctct gccagtgatc cccgcgctaa
     1741 aactaaccag tcaggggcta tttgatggcg agaagtttgc cttcactacg ctggaagtca
     1801 cggaataata aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc
     1861 actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat taccgccact
     1921 atcgccatca ggcagataca accaatcgcg gcgatatcca gcgcggcgaa ggtgcctgcc
     1981 cagccggtaa ggccgaatac cggcgtccca tcggcaatca ttcccagacc taacttggca
     2041 aagctgtcac caatcaggta agcaaaggtg cctttaatac catcggcagc gccaatcgct
     2101 tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccagg
     2161 aaacccaacg caaagagaga agccagatag atatattcgt tactggcatg ttgatacaca
     2221 ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc
     2281 gccaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc
     2341 gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc gtatacggtt
     2401 gaccactggt cgataccaat acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc
     2461 cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt catatcggta
     2521 gattctgtct ctttgtcctc ttcgctgatc tcctcgccga acagttcttc agctttgccg
     2581 aggccataag attccgggga gtcgctgccg taacgcaggc cgataaaacc gacaatcagc
     2641 gcgataatcg acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcc
     2701 ccgaacagcg ccacacctgc tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa
     2761 ccgaggaatg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac
     2821 gaaccgccgg tactctggaa aaagccgctt aaggcgtaga aggcaatcat caggaacagg
     2881 ctaaccgagc cgctgcccat actggcactg aagcccagca tacaaatagc agagaggatc
     2941 agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtagga aaccagcgtt
     3001 ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc
     3061 aacccgtagg tcgaaatcat atcgttctgc gcgatgttaa agttcttgcg aatcaggtac
     3121 atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt
     3181 ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taagaaagcc
     3241 agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc
     3301 aaatcaggcc tgagatagcg tccaggcaaa acctggaaaa tttcttagtt ttgccggata
     3361 tgaagtgaaa aggtgagatg catcacgctt cgcgcggtgt ctgggcgttc aaaaagggca
     3421 gtaacagcag tgcggaaatc ccggcggcga tagagataac cacaaaaaat ccgctccagt
     3481 gccaggtatc gagtactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa
     3541 acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacac tctgccgccg
     3601 ccataccgat taacatctgt gggccaaaga caaaaaaacc aatggtgaag aagcaggttg
     3661 cctgcatcac gtagctggca aatggcatca gccacaggga gccgactgaa agcaaaattc
     3721 cggcggcgaa aatcaaattc atcggccctc ggttgccgtt aaacaatttg tccgagcccc
     3781 aacccgctac cagcgcaccg ataaatccac ccagttcaaa catcgtcact gccgtattcg
     3841 ccgtgaccag atcgacgccc agcgtctcgg acatatacaa attgccccag tcgttgatcg
     3901 ccgcccggac cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca
     3961 gcaacacata tttggtgagg atctctttac gcgtcaaccc tgccccttct tgttgttgag
     4021 caatttccag cgcgtcgtgt cgccattcac cgaccgccgg taaacctaac gcctgcgggc
     4081 gatcgcgtag ccgccagcag agaaaaatcc ccacgactat cgccatacaa ccagcaatca
     4141 tcatcccggc acgccagccg taatgcagcg cagccgctgc catcacaatg ggaatgagtg
     4201 cgccgccgac gttatgcgcc gtgttccata atgcccacca accgccgcgc tcggtacgtg
     4261 aataccaggc cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt
     4321 tcagcaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttga
     4381 taatgcccgt ggcgataagc cctatcccca taaaataacg ggcatttgag cgatcgctga
     4441 caatgccgga gacaaacttc gacacgccat aggtaatgta aaacagggtc gctaacaggc
     4501 cgatatcgct acggctgagc acgccgttag caaggatttc tggtacggcg gcgttaaaac
     4561 ttttccgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac
     4621 gccagtagcg atagcgggca tcaatttcat atttatcagt cattaatggc gcatcggcag
     4681 gcgctttcag aaacggcaac atcatcaaac cttagacata gcgttgaggt agagaaacgc
     4741 tgacacgcgt gccgtgcaga caggaaatgt gtaatgtgcc acccagcgcc gttacgcgct
     4801 cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat
     4861 cgtcttcaat aaccagcatc aaccgttcat cctgctgcca gccttgcagg gtgaccgcgc
     4921 tggcatcagc atgtttcaca atgttgttca gcccttcctg gcagacacga aacagcgtca
     4981 cgcgctggtt ttcgcttaac gctgattcat cgattcgcca ttcgagatgg ctgacaatac
     5041 cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat
     5101 catccaactg gcgcggacgt aaccgaccca acaaacggcg caccgcgtcg taaacgccca
     5161 gcgatagttg ttcgatgagc tgcccgctct gcttcacgct ggcgttatct gccgccagcc
     5221 gctgaacaat gcccgcctga gtacgaatag cagtgatggt ctgaccgata tcatcatgca
     5281 gctcacgcgc cacatcacgg cgcacgctct cttcggtttc cagcaaccgt tcagccagat
     5341 gctgattgcg cgccagttcc ttttgcagcg actggttaag ttcacgcaac cgctggatgc
     5401 cagcgccaag caacaaccct gtcagacttt gcaccagcag cgagagcaat aaatccaccg
     5461 gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaac gtcgcaatca
     5521 gcgccccttg ccaaccatag tgccaggcca gcgcgataat cggcagcgcc agacagaatg
     5581 gcgtaaagcg cgacagttcg tccggcaatc ccaactggag ccagagactg ataacaaaca
     5641 gcagcaagta ccagaccaga tgtcgcccgc gccagttgat tggctgagaa accagtgacg
     5701 gaccgagcgg cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg
     5761 ccagcgtcag gccgccagtt aaagtcagca acagcgcatt ccacgactct ttgccgtgcc
     5821 aaagccaggg cagcgactgc aacaacgccg ccgccgttaa cgccgccccc tgtaacagca
     5881 aggtgcgcca gtcacgctga tggcgatagc gcgagatcag cgctacgggc agtaacgtca
     5941 gtaaactacc gatcatcaat aacggaaaat gggttaaacc gaccgcctgc gttagccagt
     6001 aaatcagcag ccactccgcg cccagcaata cgggccagta tccgcgcggg cattgcagca
     6061 ttagccccag acgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca
     6121 gatgcaggct gatactccac aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa
     6181 taacggtaat taagcgggag aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc
     6241 cagctctacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac
     6301 cgttttcggt gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag
     6361 tttttccgcc acctggcgtt cacgtttggt tagcgggtcc tgacgaccgg atgccagttt
     6421 aatggcaata tccggcgtca gataacagcc gcccgtggca accgtatgca ccgcagcaat
     6481 gagttcatcc gggctacagc gtttggaaag aaagccgcgt gcccccgcgt taagcgcctg
     6541 ctcaaccagc gcaggactgt cgtgaacgga gagcataatc gtcgccatac ctttcggcag
     6601 ctggcttagc agctccagac cggagatatc gggcatggag atatcgcaaa tacacacctg
     6661 cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac
     6721 ctgcaaatca ggttccagcc ccagcagctg cgcaaagccg gagcggacga tgaggtgatc
     6781 gtctataagg gcaacggtga tcatggtctt gtcctggcgg gtaaaaaaaa tacgcgctta
     6841 ccttaacgat aagcgcgatg ttgttcaagc cttgagcggt tactgaaaaa acaccgcgat
     6901 cttgttaaac atcgtcggat cggactgatt acgctgcact ttcacgacat cttccagctt
     6961 atcgatttgg cttatcatct gctccagacg ctggtcgtca ttgaccagta gccagatatg
     7021 gcttttgtcg ctgtcctgaa tcggcagaca aagaatgcct tcaacgttaa aagcgcggcg
     7081 ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg ttgcgaacgg tgagctccag
     7141 aattacgttg tcatgagttg tgttttgcat ggcttattcc cccaccattt cagtattcgc
     7201 cgcacctggc ggcaccatcg ggtaaacttt ttcttcggca tcaatgcgca catggatcag
     7261 cgccgggcca gggcgattga tgatttcctg caatgaagcc tgcggatcgg cttcgttatt
     7321 caaatcacag gtttcgaggc cgaatccggc ggcaatctgc ataaagttga ttttgcccgg
     7381 ataggtggcg gcaaaaacgc cttgctcgta gaacagactc tgttgctgat gcaccagccc
     7441 cagcgcttcg ttgttcatca gaatgatttt gacatccagc tgattttcac tggcggtcgc
     7501 catctcctga atattcatca tcaggctgcc gtcgccggag aaacacaaca ctttgcgatc
     7561 cgggttcgcc agcgcagcgc caatcgccgc aggcaggcca aaacccatcg tgcccagccc
     7621 accggaggtc agccactggc gtgggcgatt gagcggataa gcttgcgcgg tccacatctg
     7681 atgctgacca acgtcggtgg tgataattgc attgtcatcg acacaggcgg caacggcgtt
     7741 gatcaggccg taatggctta acggatcgca cgctttcggg attggacacg gaaactcacg
     7801 ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt tgcgcttcca ccagcgggat
     7861 caactgcgcc agcacgtcat caacatccgc ctgaatcgcc acgtgcggct gcttgatttt
     7921 acccagctct gcacggtcga tatcgacatg aatgattttg gcattcggac agaactgctc
     7981 ggttttgcca atcgcccggt catcaaaacg cgcaccgagc actatcaaca aatccgcctc
     8041 ctgcaaaata tagttggtgc tgcgcacgcc gtgcatcccc agcataccca gcgacaacgg
     8101 atgcgctttt ggcaacatgc ccagcgccat taaagtcatg gtggtaggca gttgcgcttt
     8161 ctccgccagt tcacgcaccc gtgcgggcgc attgatcaca ccgccgccca gataaagcac
     8221 cgggcgtttg gcagcgttaa tcatcgccgc tgcgtcacga atgctttctt cgctaaaggc
     8281 gggggcggcg gctttttctg ccatagcggg ctgtgtttca atctcaaaaa ctgccgtttg
     8341 cacatcctta ggaatgtcta tccacaccgg gcctgggcgg cctgattgcg caatgcggaa
     8401 ggcatcgctc atgacctgcg ggagttcttc gatatgtctg accagatagt tgtgtttggt
     8461 gatggggata gagatgccgt aggtgtccac ttcctggaag gcgtcggtgc cgatcatcga
     8521 ggcgggaacc tgaccagtga tgcaaatcag cgggatggag tccagccgcg catcggcaat
     8581 ggcggtcacc aggttagtcg cacccggtcc gctacaggcc atacagaccg ccggtttacc
     8641 gtcggtgcgc gccatcccct gagcgataaa tcccgcgccc tgttcatgac gggccagaat
     8701 atggcggatt tgcgtgcttt ggcttaaggc atcgtaaaca ggcaggatag aaccgcccgg
     8761 aatgcccgtc acaatcttaa tgccctgctg ttccaggaaa tgaacgataa attctgcgcc
     8821 ggtaaagcgc ttacgcgtcg atgttgtgcc cgaacttgcc atgctccagt ccttttcttc
     8881 tgggccgact ttccgggagg tgcttaaacg aaaaaccccg cccggtttgc gccggcgggg
     8941 ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg ccgacgacca ccaccacacg
     9001 cacgacgacc actgcggcgg atagcgcagt tggtagtagt tttgcgttga acatggaagt
     9061 agtcattggg gaccttgttg gttttgtgtt taacaatatt tatacaagca cagctttaca
     9121 ggggagacaa tggaaaattt ttcagcaagg gaaaattgag gggctgatca cgttttgtac
     9181 tgaattgcag ataacaaaaa accccgccgg agcgaggttt cgtcagtcgc ctgcggctgg
     9241 taaccgcaaa gcacactgta ttatgtcaac actgaaagta tacgtgttcc gcgcagaacg
     9301 cgcaatttcg gcacgaattt tgacgtattt agtgcatagt tgagtatcga tcacagtttg
     9361 cgttttgtcc aaatattact gtttatttat acagtaaact tctataatat cactgtacgc
     9421 aatgtgttat gcgggggccg catcgttacc cggcgcacta agtcctggct gaaacgggtg
     9481 gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt atgtcaacaa gcacaacgtt
     9541 tctccttgag ataccgcgtg cacaacagct ggcaacaggc agcggaaagg tacgtcagct
     9601 ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg tggatatcgc cattcttatc
     9661 ctcaaactca ttgttgcagc actgcaactg cttgatgctg ttctgaaata cctgaagtaa
     9721 ttcagattca agtcgcacca aaggggagcg ggaaaccgct ccccttttat atttagcgtg
     9781 cgggttggtg tcggatgcga tgctgacgca tcttatccgc cctaccatct ctcccggcaa
     9841 catttattgc cgcttttgtt tacatattct gccgctaaac aattccccat tcctggcgta
     9901 tatctggcta acattcatca atgtgataga ttcctctccc gcatttatgg gaatgcgtag
     9961 tgacttattc taattatttt tataaaagca tccgtgataa tgaaaaggca aagaaacgtc
    10021 aatttgttat tgatgttggt attactcgtg gccgtcggtc agatggcgca aaccatttat
    10081 attccagcta ttgccgatat ggcgcgcgat ctcaacgtcc gtgaaggggc ggtgcagagc
    10141 gtaatgggcg cttatctgct gacttacggt gtctcacagc tgttttatgg cccgatttcc
    10201 gaccgcgtgg gccgccgacc ggtgatcctc gtcggaatgt ccatttttat gctggcaacg
    10261 ctggtcgcgg tcacgacctc cagtttgacg gtgttgattg ccgccagcgc gatgcagggg
    10321 atgggcaccg gcgttggcgg cgtaatggcg cgtactttac cgcgagattt atatgaacgg
    10381 acacagttgc gccatgctaa cagcctgtta aacatgggga ttctcgtcag tccgttgctc
    10441 gcaccgctaa tcggcggtct gctggatacg atgtggaact ggcgcgcctg ttatctcttt
    10501 ttgttggttc tttgtgctgg tgtgaccttc agtatggccc gctggatgcc ggaaacgcgt
    10561 ccggtcgatg caccgcgcac gcgcctgctt accagttata aaacgctttt cggtaacagc
    10621 ggttttaact gttatttgct gatgctgatt ggcggtctgg ccgggattgc cgcctttgaa
    10681 gcctgctccg gcgtgctgat gggcgcggtg ttagggctga gcagtatgac ggtcagtatt
    10741 ttgtttattc tgccgattcc ggcagcgttt tttggcgcat ggtttgccgg acgtcccaat
    10801 aaacgcttct ccacgttaat gtggcagtcg gttatctgct gcctgctggc tggcttgctg
    10861 atgtggatcc ccgactggtt tggcgtgatg aatgtctgga cgctgctcgt tcccgccgcg
    10921 ctgttctttt tcggtgccgg gatgctgttt ccgctggcga ccagcggcgc gatggagccg
    10981 ttccccttcc tggcgggcac ggctggcgcg ctggtcggcg gtctgcaaaa cattggttcc
    11041 ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg gtcagggcag cctggggttg
    11101 ttgatgacct taatgggatt gttgatcgtg ctgtgctggc tgccgctggc gacgcggatg
    11161 tcgcatcagg ggcagcccgt ttaagcgcac gtcaccgcag catcgtcatc agctccatgg
    11221 gagaacgatg ctgctttatc agatcacgca tcacccgcat atgcggtgcg gagtaagaat
    11281 aaaacgcctg atagcccgca caaagcacgc tgtctggcgt ctcctgccga gcgtgcgggc
    11341 acaggcgatg ccagtcgcac gcctggcagt cacagcgcgg ttgcgggttt tcgctgccac
    11401 accatttttg taaggtggcg tcaaaaagct caatcgagat acgactgata tctgcccgca
    11461 cccaacactc gaacagggca attaaaaatt tcccccacag ctctgctgtc accgaagcgg
    11521 ccgtcagatc gccccggtca tcaaattcaa gatagggaat gagctgtacg gagcggatgc
    11581 cccgctgttt gaaattgtga taaaaatcgg caggttgcag agcatccttg tactctacaa
    11641 ccagtttatg cctgtcctct tcagcatcaa tgacctgact tcctgtcatg cgactctctc
    11701 aataaatacg ataagttgat gtatatgcgt tacagcaaac agtattgcga gagataacac
    11761 cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat tgggaaaaat cgctattcgt
    11821 gacatccatt aaattgcgat tacgggtgta gtgccagagg atcagcgcca cgatggcgag
    11881 aatgccaatc gaaatccaga ataacatgcc cgcctggtgc cagttgtgtt tgatagccaa
    11941 cgccatcaac gcgccgtagc ccagcatggt acgaaaccac gccagtgatg tgcgctccgg
    12001 ctgcagtcct gggtcggcaa tgcgtctggc tttgcggcta tccggcatac aacaccagtc
    12061 ccataacgat caccgccacg accatcaata ttaagctaat gattaataag ctgttggtgt
    12121 agggcaggtc ctctttcaga cgcatcgctt tttcattgcg taaccagcgc aagtagccat
    12181 acattgctaa cccgccggaa aacagacaca acaacaacgc cagcagttca cgaatgacgg
    12241 gcgtggcgaa atctggcgca agttgatcaa gcccaacccc cgccgccaga aatcctagtg
    12301 cagtacggat ccacgccaga aaagtacgct cattagccag cgagaagcgg taatcaggcg
    12361 cttctccgag gcgggaaatt ttcattgttg ctcctttgca atttcactaa cgctctacct
    12421 ttagcggttt ttttaagaat ttcgtccgcc cgtttcctga tatgcttttg ccatccgcta
    12481 caaggaggtc agatgggtat catagctcaa aataaaatca gttcattggg catgctgttc
    12541 ggtgccatcg ctttaatgat ggggattatc cacttttcat tcggcccttt ctccgcacca
    12601 ccgccaacgt ttgaaagcat cgtggcggac aaaacggctg aaattaaaag aggtcttctc
    12661 gccggaataa aaggcgaaaa aattaccacc gtggagaaaa aagaggacgt agatgtcgat
    12721 aaaatcctca atcaaagcgg tatcgcactg gccattgctg cgctactttg cgcatttatt
    12781 ggcggtatgc gtaaggaaaa tcgctggggc attcgcggcg cgctggtttt tggcggcggg
    12841 acgctggctt tccatacctt gctttttggt atcggcatcg tgtgcagcat tttgctgata
    12901 ttccttatct tttccttcct gaccggcggt tcactggttt aagcagcctg aataatgccc
    12961 gccgggtgat catcagcggg cattatcgtc agaacttctg ttttttctcc tccaccttca
    13021 ccccttgtgt cggtaggccg gtgtcatagt cgcgtacaac tggcgaatag ccatcttgtg
    13081 gacgtggacg aaacgccccc atccagcgcg gccgtgcatc tttacgccac ggacgcagac
    13141 tccattggta actgcggaac ggatcgcgaa ttttgtccat gtaatccaat aaggcgtcat
    13201 gcattttgct gcgaacgtct gcaaaacgga tatcatcgat caggttatgc atttcatttg
    13261 ggtcattacg gcgatcgtaa agttcatcac tggtgaagag gttgagtacc agtttaaagt
    13321 catccgtcac ccagcaacgc accggaataa aaccgccaaa gctgtcatgc tcaatctcgt
    13381 agcggttaaa ttccaccatc acgccgcgtg gctctttcac ggcaaggata ttttcccccg
    13441 gcagaatctc tggtttttca atatctgcca gcgccatcat tgtcggcagt aaatcgatat
    13501 gactgactgg cgtatcgacc tgtcgccgct ccccttgcgg cgaacggatg atcagcggaa
    13561 tgcgggtgat gtcgtcatac atcgccgccc ctttactgat cagcttatgt gcgcccatca
    13621 tttcgccgtg atcggaggta taaataaccc acgtattttc acgttgctct ggcgttaagg
    13681 cattgatgac ccgtccgatt tggtcatcaa caaagtcatt acaggcaaaa tagagcggat
    13741 ggtgataaag cccgtcatca ccgactggcg atggcatcgc ctgcgcccat aagcggtgat
    13801 gttccggttt gttagccagg tcatcctgtg ctttctcgcc cagctcgtag taaaaatcag
    13861 cgtatttctc taaatactcc accggacagg tgaacgggtg atgcggctca tcatacgaaa
    13921 ccaccatcag gaaaggctcg tctgcgcgcg caggctgttg cagaaaatcc accgcccgat
    13981 tgctgatacg atgcgcccag gtgaaggttt cgtcgatatg gttcgcctgt aaatcttcga
    14041 cgctgtttag gccattgcgc cacaggctaa tctctttttc cgtcagttcg ctaagatagt
    14101 tcgccccatc gaaccagtaa tcagcgtccc actccggcgg acactcgcca gtgccgaaat
    14161 agtcatgacc gtcgagatgc catttgccga tgtaacaggt gtgatagccg gcatccttaa
    14221 agtagcgccc catagtggag atgtttttgc ctggcgcgac gttgttggtc cacgggccgg
    14281 actggttagc gtagataccg gtaaatagtc cggcccgtgc aggcgtacaa accggtgaac
    14341 aggtgtaggc ggaattaaag cgaatacctt ccgccgccag actatcaata ttttgcgtat
    14401 tcagcggttt accgctatag caaccgacca tattggtggc ctgggtatcg gtcatgacga
    14461 acagaaaatt ggggcgtttc ataggctatc cttcccgtcg ggagtaaagg ttacggctgg
    14521 gtcgtgacga tgccgccagc tgtcaaaaat caggtagaca atcactacgg cggcaatgaa
    14581 ataactgatc atcgctaacc agcgcgtacc gtagccgccg aattcagcca gcccggcata
    14641 gacgccaatc atcgcgaaca ggatgccaat tgacgcgatc ttgacgtttt tccacggttt
    14701 catgtccacc gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcggtt tgataaaacc
    14761 gatcaccagc atcacgacca cgttgatgca gaacgtacag gccagcacat agaggaaatg
    14821 gaagtcgaac ttcaccagat agttgatggt gatgtagctg attatgccaa tccccatcgc
    14881 tacttttgcc gccagcgccg ggatgcgcgg gaagaaaaag cccataatga tgatggtaac
    14941 cagcggcacg ttgtaaatgc cgttgagctg tttcatccag ctatacagcc cctgcggcgc
    15001 gttggcgatc cacggcgcga ccagcaccga aacgatagcg ataaagaaac caaatttgcg
    15061 cccgacggtg accagctgct gcggctcggc attctggtta atgatgcgac ggtaaatacc
    15121 catactgaat aacgtactgg cgctattcag aaagccgttg aaggtactga tcaccgcacc
    15181 aaataacacc gcgccgaaga aacccaccat tggcactggc agaacgttat tgaccagcgt
    15241 cgggtaggcc atgtcggctt tcggtaaatc ctgatacaga tgaaacgcaa tcaaccctgg
    15301 cagcaccagt accagcgggt cgagcatttt cagcaccgcc gttaacagcg cccccttttg
    15361 cccttccgcc aggctttttg acgccagcgt gcgttgcacg atgccctgat ttgtacacca
    15421 gtaaaaggtg ttcaccagaa tcaaaccggt aaatgccgcg ccaatcggca agggatcggt
    15481 cgggccaccg attgagttta atttctcggc gtgaacggtg gtgagttgct caatgccctg
    15541 cataaagctg cccttgccca tcgcgatcag gccaaatacc ggcaccatca acccgccgat
    15601 aaccagccca ataccgttga tggagtccgc cactgccatt gcgcgcagtc cgccgatcac
    15661 cgcatacaaa attcccgcca gaccaagcaa aattaccagt agccagatag ccgcaccgtg
    15721 agaaatctgt agcgattccc cgacgtgaaa caggctgttg agcgccaacg cgccggagta
    15781 gagaacaatc ggcagaaagc agacgccggt ggcaatgagg aagcagaagt cgatgataat
    15841 acgcgtcgtt ttatcataac gttcctccag aaaatcgggg atggtggcaa tgccgcgctt
    15901 cagatagcgc ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc
    15961 ccagcccatc accgacatgc cgcttttgta ggcctgcccg gaaaggccga ccagttgttc
    16021 cgtggaaagg ttggttagca ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc
    16081 ggcaagaaaa tagccttgtt gcgatccggt atctgttttg cggaccttcc accaggtgat
    16141 caccgccacc agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat tcatctcata
    16201 gcccttattt atgtttatgt cccggacgca tgaatatagg gagaggtaaa atgcgtcggg
    16261 tcaggcagtc agattcttaa aatacaaacg tcgtatccct gaacggattg ttttctgtta
    16321 agttcaggtt gtgagcataa ttcgccggag ggatattaaa atgaatggaa aattgcaaag
    16381 ttcagatgta aaaaacgaaa ctccgtacaa tattccctta ttgattaacg aaaatgtgat
    16441 ctccagcgga atttctctga tctcgctctg gcatacctac gccgacgagc attaccgggt
    16501 gatctggccg cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac
    16561 cgtacaagga tgtgggaaaa ttcttttaaa gaatggcgaa caaataacac tgcatggcaa
    16621 ctgtattata ttcttaaagc caatggatat tcactcttat cactgtgaag gtttagtctg
    16681 ggaacagtac tggatggaat ttacccccac cagtatgatg gatattcccg ttggtcagca
    16741 aagcgttatt tataatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat
    16801 aacttcacca gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta
    16861 tcagtggatt tgtcttatgt acgcagatgg taaaaaagat ccacaacggc ggcaaattga
    16921 aaaattaatt gccactttac atgccagtct gcaacaacgc tggagcgtag ctgatatggc
    16981 tgccacgatc ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggcaa
    17041 gacgccgaaa gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca
    17101 acaaggaaac tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt
    17161 cagcaaagcc tttaaacata aatttggtta tgcgccgtca gccgtgctaa agaatacgga
    17221 ccagcaccca acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt
    17281 ggcctccagc aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga
    17341 caacgtaatc gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag
    17401 tttttccacc gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac
    17461 caccagtggt tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca
    17521 atttgcgcca tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca
    17581 ttcgggcgca gacgacgcgt ggcttctgcg acaattgctg ccgggttgga gtagttgagc
    17641 atccaggcgt ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg
    17701 gaacgcatgc cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg
    17761 cgcagtggga ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc
    17821 atgacaaaat caacgtcgct gaatgccact tcaggatcgg tggtgtaact aaactcaatg
    17881 tccggcgctt tctctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgca
    17941 ccatcgttat cataaaattt cagtgcacga agcgggaaac ggtcctgatt cgccaggagc
    18001 atcaacacga tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg
    18061 gtcattatac tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc
    18121 tatcgagctg ttcacgcaat tgggatacat gcagaccaat gatcacctga atggcatcgc
    18181 cactacggaa gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg
    18241 acatgtcatg cagtgcaatg cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc
    18301 cgaccccgcc cagggcttgc aggataccgg cagcctgatc cagctctttt tttggctctg
    18361 cagcggtggt ttggcctcgc gaggctttgt attcggcttt tgagtagagt ttcacttccg
    18421 catcttcacg tcccggcgtg cacatgttga attgcagaat cagggtgcgg aaaaccacga
    18481 agtacagcag ggtaaagcac aacccaatgg cgatttgggt cagcatcata tccgcgtggt
    18541 tgctgaacat cgggatccag ttttgcggta aaacctggtc aatcagacct ccgcccatgt
    18601 tgcccaccac accaaagaga tacatcacgg tcgacattga ggccgccagc acggcgtgta
    18661 ccgcaaacag caacggtgaa atgaacagga aggtaaattc cagcggttcg gtaattccca
    18721 ccagcatggc ggttaaggtt gcgggaatca gcaagcccgc cacttttacc cgattttccg
    18781 gtgcggcagt gaagtacatc gctaaagaaa tgcccacggc accaaagatt tttgagttac
    18841 cgtgcagggc aaaaccgcct tccgggaaca acgatttcag cggctcggcg ctcaaactga
    18901 actcttgcag atgctgcgcc cagtacatct gaatgccgcc ttcaacagct gccggaccaa
    18961 agataaacgg tccgtagatg aagtggtgta aaccggttgg gatcagaata cgttcgagga
    19021 aggtgtaaac ccacacccca agcgcacccg ccgaacgcag gaacgcttgc agagattcaa
    19081 tccccgtttg tacttttggc cagccgagca atgtcagcca ggcacagggg atcatcacca
    19141 ggaaggcgat aatcaccaca taagacgtcc cctggaaaat gccgagaaac accggcagtt
    19201 ttttatcgaa cagacggtta tgcagcgccg tcacgatgcc ggaaatgata attgcgccga
    19261 taatgctggt atcgagggtt ttaatccccg ccatcattgt cagaccgcta cctgccaccg
    19321 cgtcctgagt gaaatcgacg ccgaagtagc ttccccaggt cattcccatc gcgttgatga
    19381 aatagttcca ggtcaggaaa ctcaccatca ccgccagaca agcacgccct tgcgcttgct
    19441 tagcaaggcc aatgggtaaa ccgacagcaa aaatcagcgg catattacgg aataccgtcc
    19501 aaccgccctc ttcaataatg tgtacgattt gcgcgaataa actgttcgga tcggtcagtg
    19561 attccccgac aaacatcggg ttttgcagca agatggcaag acccaccaca atcccggcga
    19621 agggaaacag cagcactggc gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca
    19681 tttccacatc ctcttttctc aattctgtaa gtaggaagcc tgattggtta tgcggcaggc
    19741 ctggagcgag gataggaaga tgcgatcgac agaacatgac gacccaatgg gattcgtgat
    19801 cgctttcatg cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa gtagacatct
    19861 gtgagcactg gtacacagca tcgaacacat ctttaaaaaa aagatgtttt ttcaatcgat
    19921 taagcagaac ttgtgggcgc attacccggg cttgcaggca aaaaagagat ctagagatga
    19981 tctacaaaag cattgcggag cggttaagaa ttcgacttaa ttccgcagat ttcacgctaa
    20041 acagccttct tcccggtgaa aaaaagttgg cggaagaatt tgcagtatcc cggatgacca
    20101 tccgtaaagc gattgacctg ctggtagcgt gggggctggt ggtccgccgc cacggcagcg
    20161 gcacttacct ggtgcgcaaa gatgtgctgc atcaaaccgc cagcctgacc ggactggtgg
    20221 aggtgttaaa acggcaggga aaaacggtca ccagccaggt gctgattttt gaaatcatgc
    20281 ctgcgcctcc ggccattgcc agccagttac ggattcaaat caacgagcag atctacttct
    20341 cccgtcgcgt tcgttttgtg gaagggaaac cgctgatgct ggaagacagc tatatgccgg
    20401 taaaactgtt ccgtaatctt tcgctgcaac atctggaagg gtcgaagttt gaatatattg
    20461 aacaagagtg cgggattttg attggcggta attatgaaag cctgacgcca gtgctcgccg
    20521 atagactgct ggcgcggcaa atgaaggtag cggaacacac gccactgctg cggatcacct
    20581 cgttgtcata tagcgagagc ggggagtttt tgaattattc agtgatgttc agaaatgcca
    20641 gcgaatacca ggtggagtac catttacagc gactccaccc ggaaaagagt taaccgatac
    20701 tccagaagag caccgccagt aattgggggg tgataattcg cagaaacatc actaacggat
    20761 agacagtggc gtaagagagc gccgccgcac cactggttgg atgaagatta ttagcaaacg
    20821 ccagcgccgg aggatcggtc atggaacctg ccagcatccc gcacatggtc aggtaattca
    20881 ttttggctaa catccgcgcc agaatgccaa cagtaatcag cggaacggcg gtgatcaggg
    20941 caccataacc aatccagctt agcccttcgc cattgaccag ggtattcaca aaatccccac
    21001 cagatttcag accaacgacc gagaggaaca gcacgatccc cagttcccgc agcgcgaggt
    21061 tggcgcttgg cggcataaac cagtacagct tgccgatact gccgatacgc ccgaggatca
    21121 acgccataat cagcggaccg cccgccagcc ccagtttcaa cgcggccggg aacccaggca
    21181 caaagacggg aatagaacct aacaatacgc caagcccgat gccaataaac actggcagca
    21241 tctgaacctg ttgcagtttt tgctgcgcat tccccagcac attggcaacg gcatcaattg
    21301 cggacggacg ccccaccaga ttgaggatat cgccgaactg caggctgata tcgccactgg
    21361 cgaccagttc gaccccggca cggttcaggc gcgagataac aacgtcatag cgttctttaa
    21421 agtgcaggtc gcgaatacgt tttccgagca cgttttcatt ggtgaccacc acacgctcga
    21481 cgcgcaaatc agtgcctttc gtagacagcg aggtatcgac ctcctgacca atcaccagtt
    21541 gcgcattatg taaatccgct ggctgaccca ccagatgcag caaatcgccc agttggataa
    21601 tggtatctgg cgaaggaact tttagggttt cttcgcgttt cagacgcgag cagataattt
    21661 tgtcgccgtt gagaatcggt acatctttaa tggctaaatc atgcaggtta gggttctcaa
    21721 cgcgaatatt gatagtcttg atcagcgcgc cgccattggt gcgtgaagac tcgtgctgct
    21781 gagcttctgt ctcgacattg acgcggaaaa taacccgcaa catccacatg gtgaacaaaa
    21841 tcccgcaaat gccgaatgga tacgccatcg cgtaactcat ccccatctga tcgaccattt
    21901 ccattggtgt acccaggtcg cgcaaaatct gctgccctgc ccccagcgct ggcgtattgg
    21961 taaccgcacc ggagaaaatc cccagcacta ccggcagtgg aatatcaaac agtttatgca
    22021 ggatggcggt aaccagacca ccgatgatga cgatcagaac agcaaacagg ttgaggcgta
    22081 atccggagac gcgcaatgag gcaaagaagc ccggccctac ctgaatcccg atggtataaa
    22141 cgaacaggat caggccaaat tcctgaataa catgcagcat atcgctactt aatgtcatcc
    22201 ccgcctgaga aacaaaatgg ccgacgatga tcccaccaaa cagcacgccg ccaataccta
    22261 atcctatgcc gcgaaatttg acgttgccga taaacaaacc gacgactgcc accaaagcca
    22321 gaatactgac cgttaatgct atatcactca tcgtccattt ctcttgagat aacattttaa
    22381 ttgctacgga ttatggctga atcggtgaag cagatttgcc ttatagcgca cattatcacg
    22441 ggaaagaacg tgccgaaata tcttaaacag tcgaaattta acgcattgtt gagtaagtaa
    22501 gacaaaaaaa gccccaccag aatggcgggg caaagagaat agctagttag ctatttaacg
    22561 cgggacgttc gctgatagcg atacgctgcg ctgcgatggg ttcaggctca ttacgaatta
    22621 aatcaatatg cagtaaaccg tttacgaagg ttgcgccaga gacttccata ttttcagcca
    22681 gcgtaaagct caggctaaat ggctgattca taagcccttg atgcagccat tttttctctt
    22741 cttttggctg ctccggcgtg ccttttacgc tcaggcgcgt accttccagt tgaatctcta
    22801 aatcttcctg acggaaacct gccagcgcaa gggtaatgcg gtagtggtta tcgtcgcttt
    22861 tctcaatgtt gtacggcggg aagctctggc tttcaccggc gttttgcagt gcgttggcca
    22921 gtttgtcaaa accgatccat tgacgcatca gtggggataa atcgaagtta cgcatagtca
    22981 tttctccttc taagaagcga gtaagtacct gcaaatccga agattcgcat atgctccctg
    23041 acggcgagca tggagatgtc aggccgcgcc aggcggcctt agggaattag ttgatttcga
    23101 tacggcgcgg ttttttcgct tccggaatca cgcgttcgag atcgatatac agcaaaccat
    23161 ttaccaggtt agccccacga acatgaatgt tctcagctaa ctggaatttg cgttcaaagt
    23221 tgcgttcagc gatgccctgg tacagatagg tgcgctcttt ttgttcgtcg gcgtgagcac
    23281 ctttcaccac cagcagatta tcctgggcgg taatttccag ttcgctctca gcaaaaccag
    23341 ccacagcgat agcaatgcgg taatggtttt cgtctaccag ttcaacgtta tacggagggt
    23401 agccgccatt actctggctc tggttgtttt ctaagtggtt aaacaaacgg tcaaatccaa
    23461 tagcagaacg gtaaagcggg gataaatcaa agttacgcat aatcaatagc tcctgaaatc
    23521 agcgagaatg taagaccttc cacaatggac aggtcaggta gccagaacac ccatcaggcg
    23581 cgtcctcatc ggctacgatg taaaaatggg tctggaaatg aacttttcaa gccttatcag
    23641 cggacttttt tttgcagttt atggtctatt gcatagactg agggggcagc acgaaacgtt
    23701 aaagtgcgat tgccgccaca gagcgacgta atgatgatgt tattttcagc aagggatcgc
    23761 tataactcat caggccaaca cccacgatca acagatgaat ttattatgat aagaaatgtg
    23821 ttgttagcgt tcatgatatg cagcggaatg acattactcg gaggatgctc cagcgtgatg
    23881 tcccataccg gggggaaaga aggaacgtat cccggcaccc gcgccagcgc caccatgatt
    23941 ggcgacgatg aaaccaactg gggcactaag tcgctggcga ttctcgatat gccgtttacc
    24001 gctgtgatgg acacgctttt actgccgtgg gatgtgttcc gcaaagacag ctcggtgcgc
    24061 tcacgcgttg aaaaaagcga agccaacgcg caagccacca atgcggttat tccaccggcc
    24121 agaatgcctg ataactgatt tagcgatcag tttcggtgat ccacagctgg ccaccttcca
    24181 tccacgccag ccattgacca tccggtgaga agaccacggc atccgcagaa ggcggattgg
    24241 cgtggttttc ggttaaatac tcaacctcgc cgctttgcgc atgggcacaa gcaattcgat
    24301 tatccagcac aaagcccaac cattctcctg atggatgcca gttaaatgca gactgaatat
    24361 ccgttttgtt atgggttaac tggcgcggct cgccgccctg tggcgagata agccatagtt
    24421 gcacaatgcc gttatcatca cgcattaaaa acgcgatttg cgtaccctgc ggattacagc
    24481 gcacccagtg gcgcgggacg ttgactaacc ccggataagc ccgatggtgg gtaaaggtta
    24541 aacgtcgctg cacgacgccg cgcggtggcg cgggcagggt ggtttccgtt ccacttaacg
    24601 gcgcatcacc tgccgctttc cagccagctt catcttgcgg taactcaacg ataaacagct
    24661 ccggcacttt ctcgcccttt ggcgaaagtg tgtcgccaat aaacgccagc gtgtgatttc
    24721 ctacccatcc ttcttcataa gcacgattga tttcatcact gcctggctgc ggcgtgggcg
    24781 tggttttact caccagcacg caccagtggc taccgctgta ctcacgcgga tgctgctttt
    24841 gtacattgac cgggccaaac ggtgcagcaa caccgacgtt tcgcaaatcc agcgccggat
    24901 cgagttgatg cattacatgg tcgttatagg taaagctcac cctttcaccg ttcgggctaa
    24961 agacatgcac atggctgccg ccgcgcagcg ctccaggcgt atacggcgcg gtaatatcca
    25021 tcgcatcaag gttactcatc ttgccgcctt caacaatcac tccgcgccga tgatggaagt
    25081 cgtaatgcca tgtttcatca ggattttccg ggccgtggat gaagacatac ttctctgact
    25141 tcggatgaac ggtcaccacg ccgacgtgtg cgccctgtga cgcgcgatag ataacctcga
    25201 cctcgccggt atggatattc acacgctcaa tgctctctcc ggtaaacgac gcgccagaag
    25261 gacgcacgtc aaataccagc cactggctgt cgggcgtcca ggtattggta ttggtgagca
    25321 ggtgattacg gggagcaaag gttatctgtt tcatggcgcg gtcctgatac aaaacgggtc
    25381 ccgccatcat acttcacaag gacttcactt tcagacaata gtcttttgcc atcgtgatga
    25441 atgtgagtga aaagatggaa catttcgacg tggcgattat tggcatcggc ccggcaggat
    25501 cggcgttggc gcgaaaatta gcaggcaaaa tgcaggtgat cgcgctggat aaaaagcacc
    25561 agtgtggtac tgaaggtttc agcaaacctt gcggcggtct gctggcaccg gacgcgcagc
    25621 gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt gatcgccaac ccgcagattt
    25681 tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg taactaccag cgaagctata
    25741 tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc gctgatcccc accagcgttg
    25801 aggtttatca cgatagcctg tgccgaaaaa tctggcgtga ggatgataaa tggcatgtca
    25861 tttttcgtgc agacggctgg gagcaacata ttaccgcccg ctatctggtc ggtgccgatg
    25921 gcgcaaactc gatggtgcgg cgacatctct acccggatca tcaaatccgt aaatatgtcg
    25981 ctatccagca gtggttcgcg gagaaacatc cggtgccgtt ctactcctgc atctttgata
    26041 attcgataac taactgttat tcatggagta tcagcaaaga cggttatttt atctttggcg
    26101 gtgcttatcc aatgaaagac ggtcagacgc gtttcacgac gctgaaagag aaaatgagcg
    26161 cctttcagtt ccagtttggt aaggcggtga aaagcgaaaa atgcacggtg ctgtttccct
    26221 cacgctggca ggattttgtc tgcggtaagg acaacgcctt tctgattggc gaagcggcag
    26281 gatttatcag cgccagttcg ctggagggga ttagctatgc gctggatagc gcagacattc
    26341 tgcgtgcggt gttactgaag cagccagaga agctcaatac cgcttactgg cgcgccaccc
    26401 gcaaactgcg tttaaaactc ttcggcaaga tagtaaaaag ccgatgcctg accgcaccgg
    26461 ctttaagaaa gtggattatg cgcagtggta tggcgcatat tccacagttg aaagattagc
    26521 caacgcgctt cacatcgccc accagcagga tgtaagagag cgcgccaatc aacgcgacgg
    26581 cggagatata aaccagtgca ggtgcgaaac cgtaaccctg cgccaggtac cccaccacca
    26641 gcggaacggt gatgccgccc agaccaccgg cgaagttaaa cacgccgccg gttaaaccaa
    26701 tcaggcgcat cggtgccaga gaagagacca gcgaccaggt aatcgaagca aaaccgttac
    26761 cgaagaatgc cagcgccatc aggcacataa tcatcatcgg atcgttagtg tagttagcgc
    26821 ccataatgca ggtggagatc agcaagccgc agataatcgg cgttttacgc gcaaagccca
    26881 gtgaaaagcc cttacgtacc agcagatccg cgacccagcc agagagcagg acgccgacaa
    26941 acgccgcgag gaatggcacc gtggtcataa agcctgcttt cagcgccgtg attccttttt
    27001 cctgggttaa atagttcggg aaccaggtta agaaaaacca cagtgtagaa gccaccgcaa
    27061 attgcccaag ataaacgccg atcagtttac gatggaacac cagtttccag tctttggctg
    27121 ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc atccaccaga ccgccgccat
    27181 cacgaatgta atccagttca gctttgctga tacctttggt caggcgcggc ggctgataaa
    27241 ccttaaacca aatcagcgac cagataatgc cgataccacc agtgacaatg aacacccagt
    27301 gccagctcaa catctcctga atccagatca gcagcggagt caaaaacgcc agaccgacaa
    27361 actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc atgttccggg aaccagctgg
    27421 tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa aataccggtt atcgcacgca
    27481 gaccaattaa tgacattaat cccgtggcaa agccctggaa taaagtcgcc actgaccaac
    27541 caaatatcgc aataaaataa gtcacgcgag aacctacgcg atctaaaaac caaccgccgg
    27601 ggatctggca tagcgtataa agccaggcga aggccgaaaa tacatagccc atttccgctt
    27661 tcgtaatgcc gaactcttcc tgaatatggg cggaagccac ggccaggtta gcgcggtcaa
    27721 cataacaaat gaccaccgta ataaagatca tcaccagcgt cagataacgc cgacgccccg
    27781 gctttgctgc attaacggga atatccatag cgagctttct ccagattttg ggcatagcga
    27841 agccgctcac catgccctgt aatttacaga gggttatatt tttgtattgc tgttttagtg
    27901 cccgatgagg ggcttacgtg gcaggaatta ccactctgct acgctgttat cttcatgacg
    27961 ccagagcgga ttacgccagt ccggggcatt tttactgaac tcaataactt tagcttcgtc
    28021 gatttccacg cctaagcccg gtttcgttaa cggtttaaag aagccgccaa ccatgctgaa
    28081 gtcttctttg tttttcacaa agtcgagtaa ctccgcgcct ttgttgtaat gaatgcccat
    28141 actttgttcc tgaagtaccg cgttatagga aacaaagtcg atatgcagac aagccgccag
    28201 tgcaatcggt ccgagcggac agtgcggcgc aagggtcaca tcataggctt ctgccattcc
    28261 ggcaattttg tagcattcgg taataccgcc cgcgtgggag agatccggtt gcagaatcga
    28321 aataccgccc gcctccagca cgcgtttaaa atcgaagcgt gagaacatgc gctcacccgc
    28381 cgccagcgga atatgcgttt gtgccgccag tttcgggtag tattcggctt gttctgccag
    28441 caccggctct tcaataaaca gtgggcgata cggctccagt tctttaatca gcactttcgc
    28501 catcggcgcg ctgacgcgac cgtggaaatc aagaccaaac tcaatctgat tgccaaaagc
    28561 ttcacgaatt tgtgccacgg tgttgactgc cgcatctacc gcgcgggagt tatcaattag
    28621 ccccagttct tcacaaccgt tcagtttaaa ggtatcgaag ccgatttcgc gtagcgtttt
    28681 aatgccgtcg ataacatccg ccggacgatc gccgccgacc caactgtagg ctttaatttt
    28741 gtcgcgaacc aggccgccca tcagttgcca gaccggcgca ttcagcactt tgcctttgat
    28801 atcccataac gcctggtcga tcccggcgat ggcgctcatc aggattggac cgccacgata
    28861 aaatccggcg cgatacatca cttgccataa gtcgttgatg cgcgatggat cctgaccaat
    28921 caaatagtcg ctcagctcgt gaactgccgc ttccaccgtg cgggcgcggc cttcaatcac
    28981 tggctcgccc cagccgacca cgccttcatc ggtttcaatt ttcaggaaca tccagcgggg
    29041 aggtaaacga tacgtggtaa ttttggtgat tttcattgca ctgcctctcg atacgcctta
    29101 acaaatgctg ctgcctgctg cgcggtgcgt tctacggact gcccggcgcg atagagatcg
    29161 ctgcctaagc ccgccccagc acaacctgcg tctatccact gcgccaggtt ttctggcgtc
    29221 acgccgccaa cggcaaagac tgcgatgtcc gatggcaata ccgcttttaa cgctttgatg
    29281 tattgcggac caaaagccga tgacggaaat attttcagcg cctgcgcgcc cgcttcgagc
    29341 gcggtaaagg cttcggtcgc cgtcgcgcag ccggggcaga cggtcatgcc gtagcccacc
    29401 gcacggcgga tcacttcact atggatattg ggcgtaacga tgagctgaca gcccatcctg
    29461 gcgagcgcat cgacctgttc aggtttcagt accgtacctg cgccaatcaa cgccttgtcg
    29521 ccatacgcat caacgatggc gggaatgctt tgctcccatt gtggggaatt cagcgggatt
    29581 tcaaccgcgt cgaacccggc gtcaatcacc gcgccaacat gcgccagcgc ctcgtcgggc
    29641 ttaatgccgc gcaaaatggc gatcagcgga agtttagttt gccactgcat gagcgatgct
    29701 ccttatacca gcctgaaatg ccgtgtcgcc cgccaccgcc gtcacgtcgc aacccatcgc
    29761 ctgaaaggct tgctggtagc gcgcggtcag cgatgttccg gcgacaaggg tgatggcgtg
    29821 ttgatgggtc acatagtcgc gcatactggc aacctctgcg ccaatcaaca aaccagagag
    29881 aaattcgctg acctgttcgc ggggaagtgt tcccagcaca tgcgaggcgc gaacttcaaa
    29941 aagctgcggc aatatggcgg gcgcattaag gccgcgctca aggccagccg cgaaggcatc
    30001 ggcagagttt tcctgcggcg gcaaacctgc gccaatcaat gagtgattta acagtaaatg
    30061 atgtaattca ccggtcatca cggtgcgaaa atcgttgatt tgctggctat cggcctgcac
    30121 ccatttgcaa tgcgtaccgg gcataacata aagagaggaa ggagccagag ttcgcgcgcc
    30181 gatcaattgt gtttcttcgc cgcgcatcac attgtggtta tcgtcatgag agacgcataa
    30241 tccgggaata atccagatat tgtcgccaac tgacgttaat tgttcgccaa tagacgaaaa
    30301 acaggcagga acagataaat acggtgcaac tttccagccg acgttgctgc caaccattcc
    30361 tgccattacc actggcgttt tctcttcacg ccagtcggtc gtgacttctg ctaacaccgc
    30421 agccggagat tttccgttca ggcgcgtgac gcctgcttct gattgcctgc tctccaggca
    30481 gtggtcgccc tgataaagcc aggcgcgcag attggtcgat ccccagtcaa ttgcgatgta
    30541 gcgagctgtc atgtgatttc ctttaacctt cgtgtcgagc tggcgatcat ggtaagcgcc
    30601 gcctgctctg ccgcatcgcc gtcctgatgt cgtatcgcat cgaacagcgc cttatgttcc
    30661 tggagcgttt gcggcatgtt ggcctcatcg cccatccagg ttcgttcaaa aaccgcccgc
    30721 tgcagcgaac tgatcgcaat gctaagttgc tgtaacaccg ggttatgcac cgactgcagc
    30781 accgcctcgt ggtagcgaat atccgcttcg ttaaacgctt cgcggtcctg attgttggca
    30841 atcatctcgt tcagcgccga ttcaatctgc gccagatcgc tggaagtcgc gcgctctgct
    30901 gcccaacggg caatcgccgg ttccaccaga tttcgcactt cgctcatggc actgataagc
    30961 cgtgggtcgt agtcattttc cagcacccat tgcagtacgt cagtgtcgag gtaattccac
    31021 tggttacgcg gtgccacaaa cgccccgcga taacgtttca tttcaatcag ccgcttcgcc
    31081 atcagcgaac ggaacacctc acggatgatg ttgcgcgagg ttgcaaactc ctcacagagt
    31141 tccgcctcag ccggaagcgg cgagcctggc acgtatttgc cgtgaacgat ctgtttaccc
    31201 agcgtaatga caatgcgatc ggttttattg agagtcatgg agagtccttg tgctctgtat
    31261 gttcttctct actttacccc gatcgatgca taacgcggca actttgtagt accagcgtga
    31321 tgacgttcgc gtttgccgtg cgtgtaatgt agtacaaact tatattgttg tactacaatt
    31381 tagatcacaa aaagaacaat gcataaaaaa atgacatgcg tcgggcagaa atctgaaaag
    31441 ggatatcagg cgctaaacag gagggaaaga agagtatgct ttcaacggct tagctactcg
    31501 tttaaaggat taatcatgaa gttgaatttt aagggatttt ttaaggctgc cggtttattc
    31561 ccactggcgc tgatgctttc aggctgtatc tcgtatgctc tggtttccca taccgcaaag
    31621 ggtagttcag gaaagtatca atcgcagtca gacaccatca ctgggctatc gcaggcaaaa
    31681 gatagtaatg gaacaaaagg ctatgttttt gtaggggaat cgctggatta ccttatcact
    31741 gatggtgccg atgacatcgt taagatgctc aatgatccag cacttaaccg gcacaatatt
    31801 caggttgccg atgacgcaag atttgtttta aatgcgggga aaaagaaatt taccggcaca
    31861 atatcgcttt actactactg gaataacgaa gaagaaaagg cactggcaac gcattatggt
    31921 tttgcctgtg gtgttcaaca ctgtaccagg tcactggaaa acctaaaagg cacaatccat
    31981 gagaaaaata aaaacatgga ttactcaaag gtgatggcgt tctaccatcc atttaaagtg
    32041 cgattttatg aatactattc acccagaggc attccggatg gtgtttccgc agcattactg
    32101 ccagtgactg ttacgctgga catcattact gcaccgctgc aatttctggt tgtatatgca
    32161 gtaaaccaat aatcagtaag cgggcaaacg cgtttatgct gtttgcccgc ccacagatta
    32221 attcagcaca tacttctcaa tagcaaacgc cacgccatct tcaaggttag atttggtgac
    32281 aaagttcgcc acttctttca ctgaaggaat agcgttatcc atcgccacac cgacgcctgc
    32341 atattcaatc attgcgatat cgttttcctg atcgccaatc gccatgattt cttccggttt
    32401 aatacctaac acgtcggcca gtgatttcac ccccgtacct ttgttaacgc gtttatcgag
    32461 gatttcgagg aagtacggcg cacttttcag cacggtatat ttctctttca cttcctgcgg
    32521 aatacgcgcg atagcctggt cgaggatggc gggttcatca atcatcatca ctttcaggaa
    32581 ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaatgg tggcaacgaa
    32641 ggattcatgc accgtgtagt agctgatatc acggttggcg gtgtacagcg tggtgcggtc
    32701 cagggcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac gatagtcgtc
    32761 atagctgaga gcagtttgcg ccacggtgct accatcagcg gccttctgta ccagcgcgcc
    32821 gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca ggtagttgtg
    32881 cacacctgca tacgggcgac ccgtcgttag cacgacattc acgccacggg cgcgagctgc
    32941 ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa gggtgccatc
    33001 catatcgata gcaatgagtt taatagccat gagttcccca ggtagattgg ttcctgaccc
    33061 atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg gggatgcaac
    33121 gcgcgttgcg tgctcccttt ttgcttagcg gaagagtttc cctttcagca gttccatgcc
    33181 tgcggaaagc agatcgttat tggcttgtgg tgacacttca ccttgcggtg agagcgcatc
    33241 aataatcttc ggcaattgtt ctgccagtaa actggaagct gtactggtat ccacgccaag
    33301 tttttgcccg agatcggaca ccgcatttgt gccgagcgcc gattccagtt gctcgccact
    33361 aaccgattga ttgccctgtt gattactcag ccaggttgag agaatggccc ctaagccgcc
    33421 actttgcagt ttttccagca gcacctgaat gccgccctgc tcctcaaccc aacttaaaat
    33481 agcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacttcat caaaaagccc
    33541 catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatt tgcgggatga
    33601 taattgcgga ttgcagaaat aaaaagggcg gagatgatct ccgccctttt cttatagctt
    33661 cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa atcatgaaaa ttcaatacat
    33721 tgcaagattt tcgtaggcct gataagcgta gcgcatcagg cacgctcgca tggttagcgc
    33781 cattaaatat cgatattcgc cgctttcagg gcgttctctt caataaacgc acggcgcggt
    33841 tcaacggcgt cgcccatcag cgtggtgaac aactggtcgg cagcaatcgc atctttaacg
    33901 gtaacgcgca gcatacgacg actttccggg tccatagtgg tttcccacag ctgttccggg
    33961 ttcatctcgc ccagaccttt ataacgctgg atggagaggc cgcgacggga ctctttcacc
    34021 agccagtcca gcgcctgctc gaagctggct accggctgac gacgctcgcc acgttcgata
    34081 aacgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat acgacgatat
    34141 tcgccaccgg tgataaactc gtgatccagc ggatagtcag tatccacacc gtgggtacgc
    34201 acgcgaacaa tcggctcgaa caggttttgc tcagcattgg tgtgaacatc aaacttccac
    34261 tggctgccgt gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac ccagcgggta
    34321 acggtctgct catcagaaag gtcagcttcc gtcaacgtcg gctgatagat aagctctttc
    34381 agcattgctt tcggataacg acgctccata cgattgatca ttttctgcgt cgcgttgtac
    34441 tcagatacca gtttctctaa cgcttcgcca gccaatgccg gtgcactggc gttggtgtgc
    34501 agcgttgcgc cgtccagcgc gatagagatc tggtactgat ccatcgcttc gtcgtcttta
    34561 atgtactgtt cctgcttgcc tttcttcact ttgtacagcg gcggctgagc gatgtagacg
    34621 tgaccgcgtt caacgatttc cggcatctga cgatagaaga aggtcaacag cagcgtacga
    34681 atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata acgcagtttg
    34741 tccgggttgt actcgtcacg accgatacca cagccaagcg cggtgataag cgtcgccact
    34801 tcctgagaag agagcatctt atcgaagcgc gctttctcga cgttgaggat tttacccttc
    34861 agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc gcccgcggag
    34921 tccccttcca ccaggtacag ttcggaaagc gccggatcgc gttcctggca gtctgccagt
    34981 ttgcccggca ggcccgctaa gtcgagcgca cctttacggc gggtcatttc acgcgcgcga
    35041 cgcgccgctt cacgggcacg ggcagcatcg ataattttgc caaccacgat tttcgcgtcg
    35101 gttgggtttt ccagcaggta ttctgccagc agttcgttca tctgctgttc aaccgccgat
    35161 ttcacctcag aagaaaccag tttgtctttg gtctgggagg agaatttcgg gtccggcact
    35221 ttcacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc gctgactttg
    35281 gcttttttgc tgtagccttc tttgtccatg taggcgttca gggtacgggt catcgccgca
    35341 cggaagcctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt aaagcagtag
    35401 atgttttcct ggaagccatc gttccactgc aacgccactt cgacgccaat accgtctttt
    35461 tcagtggaga agtagaagat attcgggtgg atcggcgttt tgttcttgtt cagatattca
    35521 acgaacgcct tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc gcgcttgtcg
    35581 cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg tttcgccaga
    35641 atttcatatt cgaactcggt cacattggtg aaggtttcga ggctgggcca gaaacgcacc
    35701 atggtgccgg ttttttcagt ctcgccggta accgccagcg gagcctgcgg tacaccgtgt
    35761 tcgtagatct gacggtgaat tttaccctcg cgctggataa ccagctccag tttttgcgac
    35821 agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt ataggagtta
    35881 tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc cgatacgccc
    35941 tcttccgggt gaataccggt cggaatgccg cgcccgtcat cctgtacaga gacagagtta
    36001 tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc gtcgatagcg
    36061 ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt gtcgccgata
    36121 tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat actggaggag
    36181 tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat ccgttatttt
    36241 acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa cgtgttcagc
    36301 actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg caagcagccc
    36361 gcgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac accgccgccc
    36421 gctttcacgg gtgaggaact ctccttgcgc cagacgtaag gcgcacatca acagcttaag
    36481 ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc gtaagtccgc
    36541 tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg cgttcaaaat tacgttccag
    36601 cacctcagca tattctgtct ctttctccca gccgcgctgg aaagagaaag tcagagaaaa
    36661 ctcagggaga aattgcttac aggtatcagc catatcggcc gcgataccgg cgctatactc
    36721 cgcgcgccag gtgctgattt gctccgccag cgggatcagc tctttatccc acgggcgtag
    36781 ctgttcgtaa cgtgtcacct ggcgcagcgc cgcattgcgc tgcttgagca atcgcttgag
    36841 attgctccag gcggtgaaaa atccgggttc gttgtgaaag catccccagt cgaggaatgc
    36901 tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta tcaactgcat
    36961 tggcatcagg tgcgccagtt ccgcgacctt atgcccatct gtaccgtcga tgcggacttt
    37021 gctgtcgccc tgtttgtctt tggttaagcc aatcgctgtc tcgcgctctt cgccctgtaa
    37081 tcgcccgtgg agaacaaacg cctcctgctc atggcgaatg acgcgaccaa tctgcaaact
    37141 gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc agcacgctgg ttttgccact
    37201 gccgttggca cctaccagaa agttaaagcc gggagataag gcgagatccg cggtttcaat
    37261 gttgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat tggcatgaca
    37321 acataagccg cgctctggct ggccgcatct tcaatctgca cgctggaaac cgaatcggtc
    37381 agcatcatgc ggacgttttc gcatttcagc gcgttcagaa catccagcac ataactgacg
    37441 ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc ttccgcttct
    37501 tcctgttccg ggttgttggc ggtgattttc agctggtttt cgctgacata aagacgtacg
    37561 ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa acgcctgctt gagcagatcg
    37621 cagccagctt ccagatgttt gtccgggttc ttcggcagaa cgcggcgata atccgggaag
    37681 cgaccatcca ccagtttgga ggtgaagata aagtcgccaa cgtgggcgcg aatgttgttg
    37741 ctgccaatct gtacgcgcag cggattgtcg ccgccgtcga gcatacgcat cagttcaatc
    37801 acgcctttac gcggtacgat caccgaatgg cttggcaaag attgaccaat tggcattgaa
    37861 cagaccgcca gacggtggcc gtcggttgcc acggtgcgca gttcttcacc ttcggtttca
    37921 aacagcatac catttaagta atagcgaacg tcctgatgcg ccatagaaaa ctgggtcgct
    37981 tcaatcagac gcttcatcgt tgcctgcggc agggtaaatt cgacttcact ctgccagtca
    38041 tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg aaaaacggct acgcccggag
    38101 cgtaccagca tccgttcacc ttccagctgc acggcaattt ccgcgccttc aggcagacca
    38161 cggcagatat caaagaattt gcgcgccgga acggtcgtcg ctcctggctc gtgtggctga
    38221 accagcgcaa cacgtgccac catttccatc tcgagatcag taccggtcag cgacaacgta
    38281 ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg accacctaac
    38341 ggaccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt aaatttcata
    38401 ggtttacgat gacaatgttc tgattaaatt tgaaaaatct tctttgatat cgtggctctc
    38461 ttcacgcaac tgctcgatct tacggcaggc atgaagcacc gtcgtgtggt cacggccacc
    38521 aaacgcatcg ccaatctccg gcagactgtg gttagtcagc tctttcgcca gcgccatcgc
    38581 catctggcgc ggacgcgcca ccgagcggga tcgacgcttg gaaaggagat ccgcgacttt
    38641 gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca gtttttcctg
    38701 caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg cccgtccggt
    38761 aaagttggca ttggcaatga cgcggttcag cgccccttcc agctcacgta cgttagatcg
    38821 tagacgcttg gcgataaaga acgccacttc gcccggcaaa cgaatgtcgt tttcgtcggc
    38881 ctttttcatc aggatcgcca cacgggtttc cagctctggc ggttcgatcg ccacagtcag
    38941 tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt tcggatagcg
    39001 atccgaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg tgtggaaaaa
    39061 ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca gcagtgcatc
    39121 tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg ctttaaccat
    39181 gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct tgcgcgccat
    39241 aatgccgtta cccaccgcat gcagcaggtg agttttaccc agacccgtgc cgccataaag
    39301 gaacaacggg ttataggcac cgccagggtt atccgccacc tggcgagccg ccgcgcgcgc
    39361 cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt ttacgttaga
    39421 acgataggtc ggttctgccg gggccgggac gttatcccaa cctgagcgcg tagaaggcgc
    39481 agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc tcgtcactgc
    39541 cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct gtggggcatc
    39601 cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt cccgtaccca
    39661 atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca gttccacctg
    39721 caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca atcgggcaag
    39781 acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag tcgataatga
    39841 ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgct tgagggtgac
    39901 gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg atcgggaccg
    39961 cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt cccgatttat
    40021 ccacaggact ttccagaact cgctaagtgt aaacgatcct gccgcgaggc gggcacgatt
    40081 tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat cgatctaata
    40141 aagatccagg acgatccttg cgctttaccc atcagcccgt ataatcctcc acccggcgcg
    40201 ccatgctggt ttccactggt gtgaggtcgt acattttccc tgcgaaaagg tgcggaaaag
    40261 cgcggtaaat aaggaaagag aattgactcc ggagtgtaca attattacaa tccggcctct
    40321 ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc caataaagcc
    40381 attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca accgtctgta
    40441 ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa tggtcgtcag
    40501 gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa gtaataaagc
    40561 taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa ctcccagtca
    40621 attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta ccattctcgg
    40681 ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga aaaacgttcg
    40741 acgcgcccat gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc gtctgcgcca
    40801 acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg ccgacctcga
    40861 taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc gcctggctcg
    40921 cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct actcgggccg
    40981 cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg caggtttgga
    41041 gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc tttacaccct
    41101 ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta acgatggatt
    41161 cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc tggcaagcct
    41221 gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca acgaccaccg
    41281 cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa ctgatctcgg
    41341 ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt gagcaagctc
    41401 tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg ttggaaactt
    41461 caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc ccggataacc
    41521 cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg ctggctgaag
    41581 gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac acgtttacca
    41641 aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac gtgcagaacg
    41701 ctggcgagaa accgctggaa atctcctcgt ttggtcagtt gaagcaatcc atcactctgc
    41761 caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgt ggcgcggcgt
    41821 actccacgcc tgacgagaag tatgagaaat acaagttcga taccattgcc gataacgaaa
    41881 acctgaacat ctcttcgaaa ggtggttggg tggcgatgct gcaacagtat ttcgcgacgg
    41941 cgtggatccc gcataacgac ggtaccaaca acttctatac cgctaatctg ggtaacggca
    42001 tcgccgctat cggctataaa tctcagccgg tattggttca gcctggtcag actggcgcga
    42061 tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa aatggcagct gttgctccgc
    42121 acctggatct gaccgttgat tacggttggt tatggttcat ctctcagccg ctgttcaaac
    42181 tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc atcatcacct
    42241 ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc atggcgaaga
    42301 tgcgtatgtt gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat gacaaacagc
    42361 gtatcagcca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg ctgggcggct
    42421 gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg ctgatgggtt
    42481 ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca cgacctgtcg gcacaggacc
    42541 cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag aagatgtcgc
    42601 cgaccacagt gaccgacccg atgcagcaga agatcatgac ctttatgccg gtcatcttca
    42661 ccgtgttctt cctgtggttc ccgtcaggtc tggtgctgta ctatatcgtc agcaacctgg
    42721 taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc ctgcatagcc
    42781 gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg tcagttgacc
    42841 gccttttttc ttttcgtagg gcggataagc accgcgcatc cgccacacaa agcaacagga
    42901 acatcatgag cgataatgac actatcgtag cccaggccac gcctccggga cgtggcggcg
    42961 ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt tgccgaaacc gtgctgggta
    43021 aactgcctaa gccgcgctac gccgattatc ttccgtttaa agacgccgac ggcagcgtgc
    43081 tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc gaagatgtgc
    43141 tggaactgca aggtcatggc ggtccggtga tcctcgacct gctgttaaaa cgcattctga
    43201 ccattcccgg cctgcggatt gctcgccctg gtgagttttc cgaacgcgcg tttcttaacg
    43261 ataaacttga cttagcccag gccgaggcga ttgccgatct tatcgacgcc agttcggaac
    43321 aggcggcccg ttcggcactt aactcgctgc aaggcgcatt ctccgcacgg gttaatcatc
    43381 tggtagaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat ttccccgatg
    43441 aagagatcga tttcctctcc gacggaaaaa ttgaagccca gctcaatgac gttattgccg
    43501 atcttgatgc agtgcgtgct gaagcacgtc agggtagttt gttgcgcgaa gggatgaaag
    43561 tggtgattgc cggacgtcct aacgccggta aatcgagcct gttaaacgcg ctggcggggc
    43621 gtgaagcggc aatcgtaacc gatatcgccg gaactacgcg tgacgtgctg cgtgagcata
    43681 tccacattga cggaatgccg ctgcatatca tcgataccgc cgggctacgt gaagccagtg
    43741 acgaagtaga acgtattggt atcgagcgcg cgtggcagga aattgaacag gccgaccgcg
    43801 tgctgtttat ggtcgatggc accacaacag acgccgtgga tccggcagag atctggccgg
    43861 aatttattgc ccgtctgcca gcgaaactgc cgatcaccgt ggtgcgcaat aaagccgata
    43921 tcaccggcga aacgctggga atgagtgaag tgaacggtca cgcgttaatt cgtctctcgg
    43981 caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct caaacagagc atgggctttg
    44041 acaccaacat ggaaggcggc ttcctggcgc gtcgtcgcca cctacaggcg ctggaacagg
    44101 cagcggaaca tctacaacag ggcaaagcgc aactgttggg agcctgggca ggtgaactgc
    44161 tggcggaaga gttgcgtctg gcacagcaga acttaagcga aatcaccggg gaatttactt
    44221 cagacgacct gctggggcgg attttctcca gcttctgtat tggtaagtaa ccgcgcttac
    44281 gaagccgcat tctgactgtc agatgcggct tcgcttcatt gttaccactc ctgttattcc
    44341 tcaacccttt tttaaacatt aaaattctta cgtaatttat aatctttaaa aaaagcattt
    44401 aatattgctc cccgaacgat tgtgattcga ttcacattta aacaatttca gaatagacaa
    44461 aaactctgag tgtaataatg tagcctcgtg tcttgcgagg ataagtgcat tatgaatatc
    44521 ttacatatat gtgtgacctc aaaatggttc aatattgaca acaaaattgt cgatcaccgc
    44581 ccttgatttg cccttctgta gccatcacca gagccaaacc gattagattc aatgtgatct
    44641 atttgtttgc tatatcttaa ttttgccttt tgcaaaggtc atctctcgtt tatttacttg
    44701 ttttagtaaa tgatggtgct tgcatatata tctggcgaat taatcggtat agcagatgta
    44761 atattcacag ggatcactgt aattaaaata aatgaaggat tatgtaatgg aaaactttaa
    44821 acatctccct gaaccgttcc gcattcgtgt tattgagcca gtaaaacgta ccactcgcgc
    44881 ttatcgtgaa gaggcaatta ttaaatccgg tatgaacccg ttcctgctgg atagcgaaga
    44941 tgtgtttatc gatttactga ccgacagcgg caccggggcg gtgacgcaga gcatgcaggc
    45001 tgcgatgatg cgcggcgacg aagcctacag cggcagtcgt agctactatg cgttagccga
    45061 gtcagtgaaa aatatctttg gttatcaata caccattccg actcaccagg gccgtggcgc
    45121 agagcaaatc tatattccgg tactgattaa aaaacgcgag caggaaaaag gcctggatcg
    45181 cagcaaaatg gtggcgttct ctaactattt ctttgatacc acgcagggcc atagccagat
    45241 caacggctgt accgtgcgta acgtctatat caaagaagcc ttcgatacgg gcgtgcgtta
    45301 cgactttaaa ggcaactttg accttgaggg attagaacgc ggtattgaag aagttggtcc
    45361 gaataacgtg ccgtatatcg ttgcaaccat caccagtaac tctgcaggtg gtcagccggt
    45421 ttcactggca aacttaaaag cgatgtacag catcgcgaag aaatacgata ttccggtggt
    45481 aatggactcc gcgcgctttg ctgaaaacgc ctatttcatc aagcagcgtg aagcagaata
    45541 caaagactgg accatcgagc agatcacccg cgaaacctac aaatatgccg atatgctggc
    45601 gatgtccgcc aagaaagatg cgatggtgcc gatgggcggc ctgctgtgca tgaaagacga
    45661 cagcttcttt gatgtgtaca ccgagtgcag aaccctttgc gtggtgcagg aaggcttccc
    45721 gacatatggc ggcctggaag gcggcgcgat ggagcgtctg gcggtaggtc tgtatgacgg
    45781 catgaatctc gactggctgg cttatcgtat cgcgcaggta cagtatctgg tcgatggtct
    45841 ggaagagatt ggcgttgtct gccagcaggc gggcggtcac gcggcattcg ttgatgccgg
    45901 taaactgttg ccgcatatcc cggcagacca gttcccggca caggcgctgg cctgcgagct
    45961 gtataaagtc gccggtatcc gtgcggtaga aattggctct ttcctgttag gccgcgatcc
    46021 gaaaaccggt aaacaactgc catgcccggc tgaactgctg cgtttaacca ttccgcgcgc
    46081 aacatatact caaacacata tggacttcat tattgaagcc tttaaacatg tgaaagagaa
    46141 cgcggcgaat attaaaggat taacctttac gtacgaaccg aaagtattgc gtcacttcac
    46201 cgcaaaactt aaagaagttt aattaatact acagagtggc tataaggatg ttagccactc
    46261 tcttacccta catcctcaat aacaaaaata gccttcctct aaaggtggca tcatgactga
    46321 tcaagctgaa aaaaagcact ctgcattttg gggtgttatg gttatagcag gtacagtaat
    46381 tggtggaggt atgtttgctt tacctgttga tcttgccggt gcctggtttt tctggggtgc
    46441 ctttatcctt atcattgcct ggttttcaat gcttcattcc gggttattgt tattagaagc
    46501 aaatttaaat tatcccgtcg gctccagttt taacaccatc accaaagatt taatcggtaa
    46561 cacctggaac attatcagcg gtattaccgt tgccttcgtt ctctatatcc tcacttatgc
    46621 ctatatctct gctaatggtg cgatcattag tgaaacgata tcaatgaatt tgggttatca
    46681 cgctaatcca cgtattgtcg ggatctgcac agccattttc gttgccagcg tattgtggtt
    46741 aagttcgtta gccgccagtc gtattacctc attgttcctc gggctgaaga ttatctcctt
    46801 tgtgatcgtg tttggttctt ttttcttcca ggtcgattac tccattctgc gcgacgccac
    46861 cagctccact gcgggaacgt cttacttccc gtatatcttt atggctttgc cggtgtgtct
    46921 ggcgtcattt ggtttccacg gcaatattcc cagcctgatt atttgctatg gaaaacgcaa
    46981 agataagtta atcaaaagcg tggtatttgg ttcgctgctg gcgctggtga tttatctctt
    47041 ctggctctat tgcaccatgg ggaatattcc gcgagaaagc tttaaggcga ttatctcctc
    47101 aggcggcaac gttgattcgc tggtgaaatc gttcctcggc accaaacagc acggcattat
    47161 cgagttttgc ctgctggtgt tctctaactt agctgttgcc agttcgttct ttggtgtcac
    47221 gctggggttg ttcgattatc tggcggacct gtttaagatt gataactccc acggcgggcg
    47281 tttcaaaacc gtgctgttaa ccttcctgcc acctgcgttg ttgtatctga tcttcccgaa
    47341 cggctttatt tacgggatcg gcggtgccgg gctgtgcgcc accatctggg cggtcattat
    47401 tcccgcagtg cttgcaatca aagctcgcaa gaagtttccc aatcagatgt tcacggtctg
    47461 gggcggcaat cttattccgg cgattgtcat tctctttggt ataaccgtga ttttgtgctg
    47521 gttcggcaac gtctttaacg tgttacctaa atttggctaa atccttcaag aagccagcca
    47581 ttcgctggct tcttgcctct caggaaatca cttatgtcca aatggcaact cgcctgatcc
    47641 tccttcacca cgtatgcttt gcgtcacctt actatcagga cgctttagcc catgtcccgc
    47701 tttttgattt gtagttttgc cctggtttta ctttatcccg ccgggattga tatgtacctc
    47761 gttggtttac cgcgcatcgc cgccgatctc aatgccagcg aagcgcagtt gcatattgcg
    47821 ttctccgtat atctggcggg gatggcagct gcgatgttat ttgccggtaa agtggccgat
    47881 cgttcaggga gaaagccggt cgccataccc ggcgcggcgc tatttattat tgcctcggtg
    47941 ttctgttcac tggctgaaac cagcacgtta tttcttgcag gccgatttct acaggggttg
    48001 ggcgcaggct gttgttacgt agtggcgttc gctattttgc gcgacacgct ggatgatcga
    48061 cgtcgggcta aagtgctgtc attactcaac ggtattacct gcatcattcc ggtgttagcg
    48121 ccagtgctcg gacatctgat tatgcttaaa ttcccgtggc agagtctgtt ctgggcgatg
    48181 gcaatgatgg gcatcgcggt actgatgttg tctttgttta ttttaaaaga aacgcgccca
    48241 gcggcccccg cagcttcgga taaaccacga gaaaatagcg agtcgctgct taaccgtttt
    48301 ttcctcagcc gtgttgttat caccaccctc agcgtttcgg tgatcctcac tttcgtcaac
    48361 acgtcaccgg tattgctgat ggaaatcatg gggtttgagc gcggtgaata cgccaccatt
    48421 atggcgctga ccgctggcgt cagcatgacc gtttcattct ccacgccatt tgcgctggga
    48481 atttttaagc cacgtacgtt gatgatcacc tcgcaggtgt tattcctggc ggcggggatc
    48541 actcttgccg tttcaccttc ccatgcggtt tctctgtttg gtatcacgct gatttgcgcc
    48601 ggtttctcgg taggttttgg tgtggcgatg agtcaggcgt tagggccgtt ttcattacgc
    48661 gcgggcgtag ccagctcgac cttaggtatt gcgcaggttt gcggttcgtc actgtggatt
    48721 tggctggcag cggtggttgg tatcggcgca tggaatatgc tgatcgggat tctgattgcc
    48781 tgtagcatag tgagcctgtt gctgattatg ttcgtcgcgc ctggacgccc cgttgccgct
    48841 catgaagaaa tccatcacca cgcttgatct caatctgctg ctttgtctgc aactgctgat
    48901 gcaggagcgc agcgtaacca aagcggcgaa gcggataaac gtgacacctt cggcggtgag
    48961 taagtcgctg gcaaagttaa gagcgtggtt tgacgacccg ctctttgtga actcaccgct
    49021 gggtctgtcg cccacaccgc tgatggtcag catggagcaa aatctggcgg aatggatgca
    49081 aatgagcaat ctgctgctgg ataaaccgca ccaccagaca ccgcgcggcc tgaagtttga
    49141 gttggcggcg gaatcgccgc tgatgatgat catgcttaat gcgctgtcga aacggatcta
    49201 ccaacgttac ccgcaggcga ccatcaaact acgtaactgg gattacgatt ccttagatgc
    49261 cattactcgt ggtgaagtgg atattggttt ttccggtcgc gaaagccatc ctcgctcgcg
    49321 ggagctgtta agctcgctac cgttagccat tgattatgaa gtgctgttta gtgatgtacc
    49381 ctgcgtctgg ttacgcaaag atcatccggc actgcatgaa acgtggaatc tggacacctt
    49441 tttacgttat ccgcatatca gcatttgctg ggaacagagc gacacctggg cgctggacaa
    49501 tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg aatttgagca
    49561 gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc cgcgctactg
    49621 tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc cgtttgacga
    49681 aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac ggaacagcca
    49741 taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt cgatggcata
    49801 accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt tgtaataagc
    49861 ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa atgattactc
    49921 ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact ttgcccgagg
    49981 gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc atcaagaagc
    50041 ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tctagaggtt tactcgcaga
    50101 actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc tgccgaaagg
    50161 taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg ccgggaatat
    50221 ggttggcgtg gcgttaacaa ccgaaggtga atgtggtctc gatatggaac tacagcgtgc
    50281 gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca gcaatgaatc
    50341 gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca tcacgctgcg
    50401 ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg atctgcagct
    50461 gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag cgttatgcga
    50521 cgcggaagac gtgctggtgt ggtccgtggc ggtcacgccc acgattgaaa agctcagtgt
    50581 ctgggagtta gatggcaaac acggctggaa aagcctgccg gatattcaca gccgcgccaa
    50641 caatcctacc agccggatga tgcgttttgc ccaactctct accgtgaagg ctttttcgcc
    50701 aaattgatag acaaccacag gagtcatcat gtctgaaaaa ttgcaggtgg ttacgttact
    50761 ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc cgaaaattgc
    50821 tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattccct tgtatgacgc
    50881 tgacgtacag caggaagacg gttttccagc aacggttgaa gctctggcgg aacagatccg
    50941 tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac cgggtgggct
    51001 gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg gtaaaccggt
    51061 attgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt atcacctgcg
    51121 ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta tgggcggcgt
    51181 gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gaccagggta cgctggacca
    51241 cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga tctaaataaa
    51301 taacccgcca gcaatcatgc atggcgggtt tttaacgcgc tatcgatttt agtgagcgtc
    51361 gataaacaca atcttcagga taaacagcag cgcaacgatg attacgcacg ggcttagatc
    51421 acgcagacgt ccggtaccga ttttcattac gcagtaggag ataaagccca gcgcaatacc
    51481 ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg gaacagattc
    51541 agtaagatcc tgccagttca cgcgtgccag actggaagtc atcagcacgc caacgtaaat
    51601 cagcgcacca gctgcagcgt agcctggcac catccccgcc agcggcgaca gaaagataac
    51661 cagcaggaac agcagaccaa caacgactgc cgtcagaccg gtacgaccac caacagaaac
    51721 gccggaagaa gactcgatat acgccgtaac agaagaagta ccgataaacg aaccggtaac
    51781 agaagagata ctgtcgacat atagcgcctg cttcatgcgc gggaatttac ccttctcatc
    51841 cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa acaggttgac
    51901 cagcatgaaa gagaaaatca cccctgccag gccgaggtta aacgaaccgg ctaaatctac
    51961 atgacccacg acagtcatta cgctcggcgg cgcagaaacg atgccattat agtggacatc
    52021 acccagcatc cagcccagca gcgttgtcac cacgatggaa accagcaccg ccgcgtgaat
    52081 gttgcgcgag gccagaatgg cgatgatgaa gaagccgagg atccccagca gcacgctgtg
    52141 agaagtcaga ttaccgatgc tgaccagcgt ttccgggtta gcgacaatca cacctgcatt
    52201 cttcagtccc atcataccaa tgaacagacc gataccgctg gtgatgccca cacgcagact
    52261 caccggaatg ttggcaatca tccagtagcg aacgcggaaa atcgtcagta acagcagacc
    52321 tatcgcgccc cagaagattg cgcccatccc gacctgccac ggcaagccca tcgcctgcac
    52381 aacgacaaaa gcgaagaacg cattcaggcc catagcgggt gccagtgcaa ctggcaggtt
    52441 agcaaacagt cccatcataa tactgccgaa tgcagcaatc agacaggtag tgacgaagac
    52501 ggcgctggta tccatgccag caacgccaag aatttgcggg ttaacaaaaa cgatgtaaac
    52561 catcgtcagg aaggtggtaa aaccagcgat cacttcggtc cgtgccgtcg tgccatgttc
    52621 gcgcagttta aacacgcgtt ccagcatccc ctggccagaa gcctgggttg tgtgttgttg
    52681 actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata ccaaaatgag
    52741 acaataggcg cgtttgtgag agactttttt attggattta cttatacggc aacgattgcg
    52801 ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc atgtcccgga
    52861 tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc atttgctctc
    52921 gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa gaggtattca
    52981 aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt gaacatggtg
    53041 ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct cggctgttcg
    53101 attcagaact ggaagccatc gaaggggctg gagcgctcct gtcagcgatc actgcgccaa
    53161 tgtgtgtggt atctaacggc ccaaataaca aaatgcagca ttctatgggc aagctgaata
    53221 tgttgcacta cttcccggat aaactgttca gcggctacga tattcagcgc tggaagccag
    53281 acccggcgtt aatgttccat gcggcaaaag cgatgaatgt aaatgtagaa aactgcattc
    53341 tggttgatga ctcagttgcc ggtgcgcaat ctggtatcga cgcaggtatg gaagtgttct
    53401 acttctgcgc cgacccgcac aataagccga tcgttcaccc gaaagtcacc acctttaccc
    53461 atctttcgca gttaccggaa ctgtggaaag cgcgtggttg ggatattacg gcatagttct
    53521 tcacactccc ttcacttacc ccgcttaaat tggcgctcaa aggtaagtta agggtgtttg
    53581 atatgtctgt ttcacgtcgg gtaatacatc acggacttta ttttgcggtt ttaggaccgt
    53641 taattggtgt tctgtttctt gtcctctaca tattcttcgc aaaagaaccg ctggttcttt
    53701 tggtaataat acaggtactt ccattgtttc tcttattgtc gattactacg ggagctattc
    53761 ctgcattgtt aaccggcgtg atggtcgcct gtctgccgga aaagatcggg tcacagaaaa
    53821 attatcgttg tctggctggt ggcataggtg gcgtcgttat caccgagatc tattgtgcag
    53881 ttattgtaca tattaagggc atggcttcct cggagttgtt tgaaaacatt ctttctggtg
    53941 acagtctcgt tgtccgcatc attcctgcat tgctggcagg tgtggtgatg agcagaatca
    54001 ttacccgtct gcccggattg gatatttcat gtcctgaaac agactcttta agttaagcgg
    54061 gatactttat ctttgggcta ctcaaaagca gacaggatgt tactatgact caaaatatca
    54121 ggccgttacc ccaattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg
    54181 ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct
    54241 tttattgcgt tgatgaagtt gaacatctct gtccgtggtg tattgcggac ggttctgctg
    54301 ctgaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt
    54361 atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa atgctgaaag
    54421 agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt
    54481 gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg
    54541 atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag
    54601 ctaaatgcct gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg
    54661 gcaagctgag actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag
    54721 cgtaattcat acatccggcc ttatttcttc agcttaaccg agcgctaatt ctgcggcagc
    54781 ggctttatca gcgattacca tcagcgaagg atgcagctgc aacacggaag ctggaacatc
    54841 ttcggtaacc ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac
    54901 aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc
    54961 aggcactaac gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc
    55021 ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca
    55081 aaaatgacca tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag
    55141 tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat
    55201 ccccgcaggg gtgaaaaaca gattacgcag attggtaatc gttacacctt ctccctcttt
    55261 gccgcgaaag ggaatttcat cgaaattata gaaatagcag ttatcgtacc agggcttacc
    55321 tttaaccagg gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc
    55381 caggttaaca cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct
    55441 catttcctgg taatcttcgg taatgattaa tttcatctta gtgccttatt ttatttaaat
    55501 aatttctgta cgaaggctgc ataggcaggc cgccagacat ccatttcatg gttcaggccg
    55561 ggatattcct ggtaatcaaa cttaattttt ttctgctcaa gctcagtttt cagcccggcg
    55621 atatccttgc cggttacgac atctttatca ccaacgacca cagtaaaatt acgtagttgc
    55681 tggttaatag ctgccggatc gttcagccgg gccgcgacac cttcatccgg tacggttgtc
    55741 gtggtaacac cactgaatgt ggccagccag ccaaagcttt ccagatgatt cattccggaa
    55801 accagcgcct ggtacccgcc ttgtgaaagc cctgccagcg cgcggccatc ggcatcttta
    55861 cggacattaa aacgcttgct aatcagcggg ataatatcgt tcatcagttc gcgatctgcc
    55921 gctttagcat tcagcggata aaagacttta cgtctttcct gaggcacgaa atcttcggga
    55981 ataatgccat tcgcatcggt ttctgtatcc gggatcacca ccagcatcgg tttaattttc
    56041 ccttcagcaa gcaggttatc catgatttgc gggatacgcc cctgatcgat agcggaacgt
    56101 ccggtatcac caaagccgtg atagaaatag agcactggca aaggctcgcc catgccggtg
    56161 tatcctggcg gggtccagac atacatctga cgttcagatt gcaatgcgtt ggagtggtaa
    56221 gttatggcga tcaaatcacc atgcgcaaca gaacgcgtat ccagataact gcctggcacc
    56281 agaatcatac tggagttaac ctggcgctga ggcttagtca ttgcggtgcc tgtatcaatg
    56341 ctgcgtacac catcaacatt gaaaaaatac tcgtacaggt tgcctttcag gataggtgtg
    56401 cgccacgacc agactcctgc ttcgtctttg gtcatcgggt gaatattgtc cggaaccgga
    56461 acacccacca ctacagagac attttttgcc ccaggggcaa agtagcgaaa ggtcacgctg
    56521 ttatcggcat tgacctgagt cacatactgt ttcaccggga tagtgggtgc cgggctggct
    56581 ggcatatccg ctgctatggc ccactgagca gaaataccgc tggcaatagc cagcgttaaa
    56641 gcagcaattt ttatattcat cgtaatattc ctttcattaa gttccattac caccagattt
    56701 cagcctgggc accgacagca aactggtcgt ctttattgtc ttcaaaggtg aagccatcca
    56761 gttcgttttc cagggctttg atatacgtgg cgtagaaacg aatttccgga cgcgaggtca
    56821 acatactggt attgacttta aaggtatgga agagcgtggt tttataacca gactcattaa
    56881 atttattact attcgcatct ttgttttgct gggtgaaata gcccagttca acacctgtct
    56941 ggttatattg gtcccaaata taggccgggc gaacaaccgc acgaatagat tcgaaatcag
    57001 agtgtgcgcc tgtttcgtag ctataaatat cgttaccgaa ggagtaaaca atcgcgttag
    57061 ccacaataaa atgatcaccg atataggctt cgccctgcga ggtcagacga actgctgttc
    57121 cgccggtgtg atcaccataa taacgaccat taaaggtggt aaatggactt gcgccagcat
    57181 aacggccaaa gttactggcg atagagttat tcgcaaccag gaaggagaat tcgttgaagc
    57241 cacctttatc aaatttctgc gttaaagatg tgccgaacat ccaggtatct ttccagtcat
    57301 aatacccgtt attatcctga ttatcttttt ccgatgcgct ttcgtttgcc gtgacataac
    57361 gaccactcac cattaaggtc gctttatccc ataacgggat atctttatag cgtaaatcaa
    57421 tagtattggt attaatctgc tgtttgtttt gcaggctgcg atcgtaatca tcgacatctt
    57481 cgcgaaccag cgcgatatca attttacccg gaccgacttt ccagttttcc agacctacac
    57541 ccgctgcggc atcagtacgc tgcgttttcc agtccagcat ctggatttca attttcggcg
    57601 caccgtgttt acccacccag aaatcagcct ctggcgcaaa gggcaggaaa cctttggtgg
    57661 taacgtacat atcggagaac tgcataaagt tctcgccacc ggcgttatcg ccaaaccagc
    57721 cggtagagta ctgctgacca acgttaccat ccatcatcac aacggcatca acccgtttgc
    57781 cgttttcgtt gtagacacgt tgttttaact gcaaatcaaa ccagccggag tattcgttac
    57841 caaagcggcc cagagaacca atcgcccatg atttaggtga accatgagag gcggtccccc
    57901 aaccagaacg gtaatagccg ttgtaactaa agccaatttc atctttcaca aacttactga
    57961 aatctttcag agtcatggaa gaataaatcg agcctgtctc actggcattt ttctcttcat
    58021 ttttgaccag tacagcatca ggttttgcgg cactactggt cgggaacgga ttggcggcat
    58081 acccttgatc attcgtactt acgctacgat tcaccgtcgc tggcgtatat tttttcttct
    58141 cttcatcttt atactttttg agttcgcttt gcgtttctct taacgctttt tctaataact
    58201 caaggcgttg ttccaccgtt aatgattcag ccaatgattg tgcagaaaag gctaacggtg
    58261 ccattaataa gatggcagag gtaataagat ttcgtctaaa cataatatcc ctttatggtg
    58321 caaagaaaga attaacgtat cgcatccaga ctgttctgaa tgcgacgata attaaggtgc
    58381 tttattggtt atttttttaa tgacagcccc cgcgttttga tcacttctgc ataccatccg
    58441 aagcttttct tacgcgttct tgtgaggctt ccttcgccat tatcatcacg atccacataa
    58501 ataaagccgt agcgcttaga catttgtgaa tgagatgcac tgactaaatc aattggcccc
    58561 caactggtgt accccataat atccacacca tcggcaatcg cttcatttac ctgtaccagg
    58621 tgatcgttta aataggcaat tcgataatcg tcctgtatcg aaccatccgc ttcaacgctg
    58681 tcttttgcgc ctaatccgtt ctcgacaata aataacggtt tttgataacg atcccaaagc
    58741 gtatttaaca gaacccgtaa tccaaccgga tcaatttgcc acccccactc tgaacttttc
    58801 agatgcggat tggggatcat attcagtatg ttgccctgcg catttttatt aatgctttcg
    58861 tcgtgggaaa cacaaccagt catgtaataa ctaaaagaga tgaaatcgac ggtatgtttt
    58921 aaatcttctg cgtcactttc agtcatctca atggtgatat tgtggtcgcg gaagaaacgc
    58981 tgcatatagc cgggatactg gccacgcgcc tgaacatcac caaagaacat ccagcgccgg
    59041 ttctcttcca tggcctgcaa catatcctgt ggctggcagg tgagggggta aaccagccca
    59101 ccgagaagca tattgccgat tttcgcttcg gggagcaggc tatgacaggc tttaactgcc
    59161 cgcgcactgg caaccagttg atgatggatc gcctgataaa cttccgcctc gccactctct
    59221 tctgccagcc ccacgcccgt gaatggcgcg tgtaacgaca tattgatttc attaaacgtc
    59281 agccataacg ctactttatg ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag
    59341 tgatcgatga ccgcccgatt agcccaaccg ccgtagtttt tcaccagccc atatggcatt
    59401 tcgtaatggg ataacgttac cagcggcttg atccccgcct gcgccatttc atcaaacagc
    59461 cgatcgtaaa acgctaaccc cgcttcattc ggttcggctt cgtcgccctg agggaaaatt
    59521 cgcgcccagg caatggaaat acgcagacag gtgaagccca tctcggcaaa taacgcgata
    59581 tcttccgggt aacggtgata aaaatcgatg gcgacatctt tgatattctc tttccccagg
    59641 atgcgcggtt ccatttttcc cataacgcca tgaggctgta aatctgaggt cgtaatccct
    59701 ttgccatctt ccagccaggc accttccacc tgattggcag ctgttgcgcc cccccaaaga
    59761 aatgtttctg gaaatgcttt cataattaac tccttttatc gttagcgaat gatggataac
    59821 agcggttcac ctgcgcttat ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg
    59881 ctattactga ttaataccgg cgtcgtcaca tcaaatccgg cctcgcgaat agcagggata
    59941 tcaaaagaaa tcagccgatc gcctgtattg actttgtcac ccacgttgac gtgagcggaa
    60001 aagaatttgc cgtccagttt tacggtgtcg ataccgacat gaatcaggat ctccacacca
    60061 tcatctgact caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca
    60121 accggagaac gcacttcacc aaccgagggc agaatggcga tacctttacc caacaggcca
    60181 ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa tctctcccgt catcggtgaa
    60241 cagataccgc cctgctcagg tggtgtaata acctctggtg ttttctcttg cggggcaccc
    60301 tgcgctggct gacgtttagc ggtgatgaaa tgaagcatca ccgtaccgac aaatgcgcaa
    60361 ccgatggcaa tgacaccgcc aataacgctg gcccagacgg tgaaatcaat ccccgttgac
    60421 gggatggtct gcatgaaggt gaaaatactc ggcaaaccaa aggagtaaac ttttgtttgc
    60481 gcgtagccaa tgatggtggc ccccaaagcc ccactgatac aggcgataac aaaggggtac
    60541 ttacgcggca ggttgacgcc atataccgct ggttcggtga taccaaacag gcccgtcaac
    60601 gccgccgatc cggctaccac ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc
    60661 gccgccccga cctgcgccat aatggcgggc attaacagcg ggatcatggt gtcgtagccc
    60721 agcacggtga agttattgat acacagcggc accaggcccc agtgcagtcc gaacatgacg
    60781 aagatttgcc agaagccgcc cattaccgcg cccgcaaatg caggaaccgc ctgataaagc
    60841 cagagataac cggcggcaat catttcgctt atccaggttg atagcggccc caccaacaga
    60901 aaggtgacgg gtgtgataac catcagacat agcaatggtg tgaagaaatt tttgattgcc
    60961 gacggtaacc acgcattaag tcggcgttcc agaatgctgc acaaccaggc agaaaaaata
    61021 atgggaataa ccgatgacga gtaattcaac aatgtgaccg gaatacccag gaaatccagc
    61081 cccagcgcat ccgctttttg cccgttctcg aaagcagtca gaattaatgg atgcactaac
    61141 gctccaccaa tcaccatggc agtaaatgga ttaccgccga agcgtttccc cgcggtgtat
    61201 cccaggatta tcgggaagaa ccaaaacaag gcatcactgg cgctgaataa aattaaataa
    61261 gtaccacttt gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat acctttcagg
    61321 atcccggttg ccgccatcag accgatcaga ggcgtaaaaa tacctgaaat aacataaaca
    61381 aagcggttta gcagattacc tttctcatca ttttccggtg cctgttgcgc tttttcgcca
    61441 aggcctgcca cgctgttaac cgccaggaag acatcggcca catggttacc tatgaccacc
    61501 tgaaactgac caccgctttc caccaccata ataataccgg gggtcttttt cagtacctca
    61561 gcttgcgctt tgctttcatc ctttaactta aaacgtaatc gcgttgcgca atgcatcaga
    61621 ctcacaatgt tatctgcgcc cccgactcct gcgactattt ttctggctaa ctccgtcata
    61681 acttgccctc taccgctttg cggcaaaact ccaaaaaaaa acctgaaaaa acggcctgac
    61741 gtgaatcaag caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca
    61801 gtaataatat ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc
    61861 tctgcgggtg aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca
    61921 cattgccatg cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg
    61981 ttaattgaag catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc
    62041 tgataactca agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc
    62101 atttcgcgca ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca
    62161 ctgaccagat gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt
    62221 ttatcaataa tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg
    62281 atatcccaca gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac
    62341 tggcaatggt cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc
    62401 gctaaagaga taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt
    62461 tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt
    62521 gagttaattc tttcgccagc gcgtttttga aagccaattc cgcgccccat gacgactttt
    62581 tcccgctgtt gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc
    62641 atgttcatag caaggacctt ttttataaac aaaaaaaccc gacttcacca gtattctctg
    62701 gttatgtcag gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta
    62761 taacgaacat ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct
    62821 attgataaaa atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata
    62881 aagttatata taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg
    62941 tcgctatctt tccccgccag cagtttatcc agctcatcgc caccgacgtg acggaaatcc
    63001 tgccccttca cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt
    63061 tcgatagaac gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc
    63121 atcatgtagg tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct
    63181 tcacgataaa tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg
    63241 tgcagcatct ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc
    63301 tgctgggaga atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc
    63361 tccagctcgg caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc
    63421 tgacgtttgg caataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg
    63481 ttcttgtcgc cttcgatgac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg
    63541 attgcatcag aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata
    63601 ctttccagtt cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata
    63661 acgcactcct gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg
    63721 gcttggtgaa cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg
    63781 ccgtgtggtc ggaacaacgc gcagcctgct gcatgttgtg ggtgacgatc accacggtgt
    63841 aatcctgctt cagttcggtg atcagctctt caatacgccc ggtagagata gggtcgagcg
    63901 ccgaacacgg ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac
    63961 acagacgctg ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg
    64021 tttcgttcca caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac
    64081 gggagagctt ctcaaacaga cgaacgccaa aagcgatgtt gtcgtagatg gacatcggaa
    64141 acggcgtcgg tttctggaac accatgccca ctttcgcacg cagcagggcg atatcctgag
    64201 agttggtcag gatgttgtcg ccatcaagca gaatttcacc ttccgcacgc tgctccgggt
    64261 acagttcaaa cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc
    64321 cgataaacgc cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga
    64381 atttgccgta gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca
    64441 tactcattgc aatcttttcc tcatcgtgcc tgatgcactt cgcgaatcag gcttaccgta
    64501 ggccggatca ggcgttcacg ccgcatccgg caaagtgtta accgtgtaca gagtcaaaaa
    64561 ggcgctcggt cggtcccctc gcccctctgg ggagagggtt agggtgaggg gaaaaccgtg
    64621 tcagcaatat caaccgtgtt tattcttcgc gaaaacaacg cgcgccagaa tgttcagcag
    64681 cagtacgcac agggtaatga tcaatacccc ggcccaggcc agctgctgcc attccgcaaa
    64741 cgggctcatc gcaaacttaa agatcgtcac cggcaggttg gcgatcggct gcatcatgtc
    64801 cgtgctccag aactggttgg agagcgcggt aaacagcagc ggcgcggttt caccagcaat
    64861 acgggcaatc gccagcagga taccggtcat gatcccggac accgacgctt tcagcgtgat
    64921 cgcggagatc atcttccact tcggtgtacc cagcgcataa gccgcttcac gcaggctgtc
    64981 cggcaccagt ttcagcatgt tctcggtggt gcggataaca atcggcacct gcaacagcgc
    65041 cagcgcaatc acgcccgccc agccggagaa gtgctccatc tgcgccacca caatggtgta
    65101 aacaaacaga ccaaccacaa tcgatggcgc agagagcaga atgtcgttaa tgaagcgaat
    65161 cacttctgcc agccaggatt tacgaccata ttcagccaga taaatccccg ccataatgcc
    65221 cagcggcgta ccgaataccg tggcccacaa aattaacagg ccgctacctg ccagggcgtt
    65281 cgccagacca ccaccttccg tattgggcgg cggcgtcatt tcagtgaaca gcgccagcga
    65341 cataccgtcg ataccgcggg tgatggtgga cattaaaatc cagatcagcc agaacagacc
    65401 gaaggccatc gtcgccattg agagcgtcag tgcaatacgg ttcttgaggc ggcgacgcgc
    65461 ctgcattttg cggcgggatt cagccagcgc cgcattggtt tgcatttcaa ccatagccat
    65521 tagcgtgccc cctcattctt agccaggcgc ataatcataa acttcgatgc ggcgaggacg
    65581 atgaaggtaa tcacaaacag gatcaggccc agttccatca gtgcggcaac gtgcagaccg
    65641 gattccgctt ccgcaaattc gttcgccagt gcagaggtga tactgttgcc cggcatatac
    65701 agcgaggcgc tgtcgagctg gtaggtgtta ccgatgataa aggtcaccgc catggtttca
    65761 ccgagcgcgc ggcccagccc cagcatgatg ccgccgataa caccattttt ggtgaacgga
    65821 agaacgatac gccagataac ttcccaggtg gtgcagccaa taccgtaggc cgactctttc
    65881 atcatcaccg gggtttgttc gaacacatcg cgcattaccg ccgcaatgta cggaataatc
    65941 atgatggcga ggatcacgcc tgccgcgagg ataccgatac caaatgcagg gccagagaac
    66001 agcgcgccaa caatcggaat attcgacatg atattgccga ccggctcctg gaagtaaacg
    66061 gcgaacagcg gcgcaaagat aaacaggccc cacatgccgt aaacgatact tggaatggct
    66121 gccagcagct caatggcgat acccagcggg cgtttcagcc agccgggcgc aagctcagtc
    66181 aggaacaggg cgataccgaa actcaccggg acggcgatca gcagcgcgat aaacgaagtc
    66241 accagcgtac cgtagatcgg caccaacgcc ccgtagatat cgttcggtgc atcccactct
    66301 ttggtccaca ggaaagccag accaaatttc tgaatgctcg gccaggagga gatgatcaga
    66361 gagacaataa tgccacccaa catcaatagc acaatcagcg ccgccagttt taccagcacg
    66421 ctgaaaatta tgtcgccctt tttacccggt gggttaaaag caggcttggt tgcagccata
    66481 agttactctt cagttaaacg cgtttacaaa gtcattttgt aatgccggat aagccgttaa
    66541 tgccttatcc ggcctggagt tttattagta cagcggctta ccgctactgt ctttaatatt
    66601 ggtcttccac gcagcgcgaa cctgttcaac gacgctatcc ggcaggctgg cataatccag
    66661 gtcgttcgcc tgtttagccc cggttttgta cgcccagtcg aagaacttca gcacttctgt
    66721 gccttgttct ggtttcttct gatctttgtg gatcagaatg aacgtggtag aggtaatagg
    66781 ccatgcatct tcgcctttct ggttggtcag atcctgagcg aaggttttgc tccagtctgc
    66841 accttttgct gcattagcga agttttcttc ggtcggacta accggtttac catcagcgga
    66901 gatcagtttg gtgtacgcca ggttgttctg cttagcgtaa gcatattcaa cgtaaccaat
    66961 tgcacccggc agacgctgaa cgaacgcggc gataccgtcg ttacctttac cgcccagacc
    67021 gatcggccat tttacggtag agccagtacc aacgttgttt ttccactctt cgttcacttt
    67081 cgccaggtag ctggtgaaga cgaaggaagt cccggagcca tctgcgcggc gtactacagc
    67141 aatgttttgt gaaggcagtt tcagacccgg attcagtttg gcgatggctt catcatccca
    67201 cttcttgatt ttgcccaggt agatgtcgcc gagggtttta ccatccagca ccagttcgcc
    67261 agacttcagc cctggaatgt taaccgccag caccacgccg ccaatcacgg tcgggaactg
    67321 gaacagacct tcctgagcca gtttttcgtc agacagcggc gcgtcagagg caccaaaatc
    67381 aacggtatta gcgataatct gttttacgcc accggaagaa ccgataccct ggtagttaac
    67441 tttattaccg gtttctttct ggtaagtgtc agcccatttg gcatacaccg gcgcagggaa
    67501 ggttgcacct gcacctgtca ggcttgcttc tgcaaacaca gagaaagcac tcatcgataa
    67561 ggtcgcggcg acaacagttg cgacggtggt acgcataact ttcataatgt ctcctgggag
    67621 gattcataaa gcattgtttg ttggctacga gaagcaaaat aggacaaaca ggtgacagtt
    67681 atatgtaagg aatatgacag ttttatgaca gagagataaa gtcttcagtc tgatttaaat
    67741 aagcgttgat attcagtcaa ttacaaacat taataacgaa gagatgacag aaaaattttc
    67801 attctgtgac agagaaaaag tagccgaaga tgacggtttg tcacatggag ttggcaggat
    67861 gtttgattaa aaacataaca ggaagaaaaa tgccccgctt acgcagggca tccatttatt
    67921 actcaaccgt aaccgatttt gccaggttac gcggctggtc aacgtcggtg cctttgatca
    67981 gcgcgacatg gtaagccagc agctgcagcg gaacggtgta gaagatcggt gcaatcacct
    68041 cttccacatg cggcatctcg atgatgtgca tgttatcgct acttacaaaa cccgcatcct
    68101 gatcggcgaa gacatacaac tgaccgccac gcgcgcgaac ttcttcaatg ttggatttca
    68161 gtttttccag caattcgttg ttcggtgcaa caacaataac cggcatatcg gcatcaatta
    68221 gcgccagcgg accgtgtttc agttcgccag cagcgtaggc ttcagcgtga atgtaagaga
    68281 tctctttcaa cttcaatgcg ccttccagcg cgattgggta ctgatcgcca cggcccagga
    68341 acagcgcgtg atgtttgtca gagaaatctt ctgccagcgc ttcaatgcgt ttgtcctgag
    68401 acagcatctg ctcaatacgg ctcggcagcg cctgcagacc atgcacgatg tcatgttcaa
    68461 tggaggcatc cagacctttc aggcgagaca gcttcgccac cagcatcaac agcacagtta
    68521 actgagtggt gaatgcttta gtggatgcca cgccgatttc tgtacccgcg ttggtcatta
    68581 gcgccagatc ggattcgcgc accagagaag aacccggaac gttacagatt gccagtgaac
    68641 caaggtaacc cagctctttc gacagacgca ggccagccag ggtatccgcg gtttcgccag
    68701 actgtgacaa ggtgatcatc aggctgttac gacgcacggc agatttgcga tagcggaatt
    68761 cagaggcgat ttcgacgtcg cacggaatac ctgctagcga ttcaaaccag tagcgggaaa
    68821 ccataccgga gttataagaa gtaccacagg cgaggatctg aatatgctca accttcgaca
    68881 gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac ctgaccgtgg ctgatgcgtc
    68941 cggtaagggt gtttttgatc gcgttcggct gttcgtagat ctctttctgc atgtagtgac
    69001 ggtaaatgcc tttatcgccc gcgtcatatt gcagattgga ttcgatatcc tgacgtttta
    69061 cttccgcgcc agttttatcg aagatgttta ccgaacggcg agtgatttcc gcaatatcgc
    69121 cctcttcaag gaagataaag cgacgggtca ccggcaacag cgccagctgg tcagaagcga
    69181 taaagttttc gcccatcccc aggccaatca ccagcggact accagaacgt gccgccagca
    69241 gggtatccgg gtgacgggag tccatgatca ctgtaccgta cgcaccacgc agctgcggga
    69301 tagcacgcag aacggcctca cgcagagtcc cgccttgttt cagctcccag ttcaccagat
    69361 gggcaatcac ttcggtgtcg gtttcagaaa cgaaggtata gccacgcgct tttagctctt
    69421 cacgcagcgg ttcatggttt tcgatgatgc cgttatgcac caccacaatg tgttcagaaa
    69481 catgcggatg cgcattcact tctgaaggtt caccgtgggt cgcccagcga gtgtgagcaa
    69541 taccagtgcc gccatgcaga ggatgttctt ccgctgcctg tgccagcatc tggactttac
    69601 cgaggcgacg caggcgggtc atatgacctt ctgcatcaac aacggccaga ccggcagagt
    69661 catatccgcg gtattccaga cgacgtaaac cttcaagaag gatttctgct acatcacgtt
    69721 gcgcgatcgc gccaacaatt ccacacatag tttttgattc cgatttatat cgttgtcggt
    69781 caacctgtat gcccgttttt tcgggcgccc cgagccttgt agagagtggg gttattttta
    69841 tagttactgc ttgtgggagg gagattatgt tatctcctca tcccatgtga ccgggttagc
    69901 cggccagaat cactttttct ttaccggacg acgccagcct tctttctgag tctgcggcac
    69961 acggctgata gctaatgcat tttcgccgac attacgcgtc acagttgtac ccgcagcaat
    70021 ggtcgcgcct ttgcctactg ttaccggggc caccagctga gtgtcggaac caacaaacac
    70081 atcgtcgccg ataatggtct taaatttatt cgcaccatcg tagttgcagg taatggttcc
    70141 cgcgccgatg ttaacgttat cgccaatttc cgcatcgccc aggtaagtca gatgaccagc
    70201 tttcgagcct ttacccagac gcgctttttt catctcaacg aagttaccga cgtgagcacc
    70261 ttccagcaac tcagcaccag gacgcaaacg ggcaaacggg ccaatggtac aggccgctgc
    70321 cagattcgca tcttccacaa cggtatacgg actgatttcg caatcatcgc caatcacgct
    70381 gtttttaatc acgcaaccgg tgccaatttt cacgcgatga ccgagagtca cgttgccctc
    70441 gatgataacg ttagtatcaa tttcaacatc gcgcccgtga gttagcgtac cacgcagatc
    70501 aaaacgcgct ggatcgcgca gcataacgcc tgctaacagc agtttttcag cctgttcgga
    70561 ctgataaaca cgctccagac gggagagttg caggcggtta ttcacgcctt ctacttcgct
    70621 taaacgttgc ggatgaacgg cgacgatttc acgcccttcc tgatacgcca gcgcaataat
    70681 gtcggtgatg tagtattcgc cctgagcatt attgttggtc agcttcgcca gccagcgttt
    70741 catatctgcg ccgttggcaa tcagaatgcc ggtgttgatc tcctgaatct gacgctgctc
    70801 gtcggtggca tctttgtgct caacaatgcc ggtaactttg ccgttttcac gggtgatacg
    70861 tccataaccg gtcggatcat ccagtttcac cgtcagcaga ccaatgccac cctgcggttt
    70921 agcatcacgc agacgctgga gtgtttcgac agagatcagc ggcacgtcgc cgtagagcat
    70981 taaaatgtct tcatcatcgg caaagaaagg tgcggcctgc tgcattgcat gacccgtacc
    71041 cagctgctct gcctgaagca cccagttaag gttgtcgtct ttcagcgcct gttttagcag
    71101 atcgccgccg tgaccgtaca ccaggtgaac gtgcgctgcg cctaattcat tcgcagcatc
    71161 aatgacatgc tgaaccatcg ctttcccggc aagggtatgc agcactttcg gaagatcgga
    71221 atacatgcgc gtgcctttgc ctgcggcaag gatcactacg ctcatagcat tattcaacat
    71281 acgcgtcctg actgtaattt gagaacgaat ttaaaccgct tcaccttgaa aaaactacat
    71341 ttttttcatc gtgaaatgga cagaggataa attgttcaat aacggattat ccccgctgac
    71401 gaaagcgcca ttttcgacca tcgtcacgcc ttttgtctgc agaaaattaa ggcagtacct
    71461 taatttaagc gtcaggtgga tgtttttgct cttatttcga tcaatgaata aacaggaaaa
    71521 caggggggtt ttatatcagc aggatctatg tgaacgctat tcaggacggg tcacacgcgc
    71581 aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt ttcaagccgg tgttacatcg
    71641 cttttttggt caactcgata acgcgcagct gcgcgatcgc tttggccagt tccgcagacg
    71701 cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg ctcttcagcc ttacgtttcg
    71761 cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat tgcggtgtcg gccagaacgg
    71821 tcacgttgcc aggctgcact tcaagaatgc cgccagacag atagataaac tcttcgtgac
    71881 cgtgctgttt cacgatgcga atcataccag gcttaatggc ggtgagcagc ggtgcgtggc
    71941 cagggtagat ccccagttca ccttcgctac ccgttacctg gattttctcg accagaccag
    72001 agaacatttg ttgctctgcg ctgacgacgt ccaggtggta agtcattgcc atatcaccct
    72061 ccgattaagg cgttaaagtt ttttggcttt ttccacagct tcttcgatgg aaccgaccat
    72121 gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc atgatgcctt taaagccacg
    72181 gatggtgtct ttcagggaga cgtatttacc cggagaaccg gtgaatactt ctgccacgaa
    72241 gaacggctgg gacaggaagc gctggatctt acgagcacgc gctaccacca gtttgtcttc
    72301 ttcagacagt tcatccatac ccaggatggc gatgatgtct ttcagttcct gataacgttg
    72361 caggatggac tgaacgccac gcgcggtgtc gtagtgttcc tgaccaacca ccagcgggtc
    72421 cagctgacgg ctggtggagt ccagcgggtc aacggccggg tagataccca gagacgcgat
    72481 ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag gtggttgccg gagacgggtc
    72541 agtcaagtca tccgcaggta cgtatactgc ctgtacggag gtgatagaac cagttttggt
    72601 ggaggtgata cgttcctgca gaacgcccat ctcttccgcc agggtcggct gataacctac
    72661 cgctgaaggc atacggccca gcagtgcgga tacttccgta ccggccaggg tgtaacgata
    72721 gatgttgtca acgaacagca gaacgtcacg accttcgtca cggaatttct cagccatggt
    72781 cagaccggtc agagcaacgc gcagacggtt tcccggcggc tcgttcatct ggccatacac
    72841 cagggatact ttgtcgataa cgttggagtc ggtcatttcg tggtagaagt cgttaccctc
    72901 acgagtacgt tcacctacgc ccgcaaacac agagtaaccg gagtgctcga tcgcgatgtt
    72961 acgaatgagc tccatcatgt ttacggtttt acctacaccc gcaccaccga acagaccaac
    73021 tttaccgccc ttagcgaacg gacacatcag gtcgataact ttgataccgg tttccagcag
    73081 ttcctgagag tttgacagct cttcgtagga aggtgctgcg cggtgaatcg cccaacgctc
    73141 ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc agtacgttca tgatacggcc
    73201 cagagtcgct ttacctaccg ggacttcaat cgggtgttcg aggtctttta catccagacc
    73261 gcgacgcaga ccgtcggagg aacccattgc gatggtacgt acgataccgc cgccgagctg
    73321 ctgctgaact tccagcacca gacgctcatt accattttgc acctcaagag catcgtacac
    73381 gcgcggtacg gcatcctgag ggaattcgac gtcaactacg gcgccgatta cctggacaat
    73441 ctttccagta gccatcttaa atcctctacg aaataacctg tttaaaccgc ggcggccccc
    73501 gagacgatct cggtgagttc ctgagtaatg ctggcctgac gagctttgtt gtataccaac
    73561 tgcagctctt taatcaggct gccgccattg tcggtcgcgg ctttcatcgc caccatacgg
    73621 gcggcctgct cgctggccag gttttcaacc acgccctgat aaacctgaga ttcgacataa
    73681 cgacgcagca gggtatccag caacgccttc ggatcgggtt cgtacaggta atcccaggat
    73741 ttatgtttca gatcatcatc atctgatgcc ggtaacggca gcagctggct gatggtcgga
    73801 acctgagaca tggtgttaat aaatttgttg ctgacaatgt aaagcttgtc cagacggcct
    73861 tcgtcgtagg cctgcaacat cacttttacc ggaccgatca gttcggacag ggaagggtta
    73921 tcccccatgc cggtgacctg ggcaacaaca ttgccgccca cggagttgaa gaacgacacg
    73981 cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt tgtcggtcca ggtcttcatt
    74041 tccgccagca gttttttgaa caggttaatg ttcaaaccac cgcacaaacc acggtcggtc
    74101 gacaccacca gatagcccac gcgtttaacg tcgcggtctt ccaggtaagg gtgcttatat
    74161 tccagattac cgtgtgcaag gtgaccaatc actttgcgca tggtttctgc ataaggacgg
    74221 ctggccgcca tgcgatcctg cgatttacgc attttggaag cggcgaccat ctccatcgct
    74281 ttagtgatct tttgcgtgtt ctggacgctt gcgatcttac tacgtatctc ttttgcgccg
    74341 gccatgagct tctcctcaat gccttgcggc ctgccctaag gcaagccgcc agacgttacc
    74401 aggattgggt tgctttgaag gaatcgagga tgcctttcag cttgccttcg atttcgtcgt
    74461 tgtagccacc ggtctggttg atctcttgca tcaacggagc gtgatcacgg tcgacgtaag
    74521 ccagcagagc ggcttcgaag ctgccaattt tcgacagttc aacatccgcc aggtaaccac
    74581 gttctgctgc gaacagaacc agagactgct gcgcaacgga catcggcgca tactgtttct
    74641 gtttcagcag ttcggtcact ttctgaccgt ggtcaagctg cttacgtgtt gcatcgtcaa
    74701 ggtcggatgc aaactgagag aacgctgcca gttcacgata ctgtgccaga gcggtacgga
    74761 taccaccgga cagttttttc atgatcttgg tctgtgctgc accaccaaca cgggatacgg
    74821 aaatacccgg gttaaccgca ggacgaatac cggcgttgaa caggttggtt tccaggaaga
    74881 tctgaccatc ggtaatggag attacgttgg tcggaacgaa cgcagaaacg tcacccgcct
    74941 gagtttcgat aatcggcagt gcggtcagag aaccggtttt ccctttcact tcacctttgg
    75001 tgaaggcttc aacgtattcg gcgttaacac gcgcagcacg ctccagcaga cgagagtgga
    75061 ggtagaatac gtcgcccggg aatgcttcac gtcctggcgg acgacggagc agcagggaga
    75121 tctgacggta agcaacagcc tgtttagaca ggtcatcgta aatgatcagc gcatcttcac
    75181 cgcggtcacg gaagtattcg cccattgcgc aaccggcata cggtgccagg tattgcagtg
    75241 cagcggattc agacgcggtt gctaccacaa cgatggtgtt agccagtgcg ccgtgctctt
    75301 ccagtttacg taccacgtta gaaatggtgg acgctttctg gccgatagcg acatagatac
    75361 atttgatacc ggaatcgcgc tggttgatga tggcatcgat agccagtgca gttttaccgg
    75421 tctgacggtc accgatgatc aattcacgct gaccacgacc gattgggatc atggagtcaa
    75481 cagctttata accggtctgt accggctgat ctacggactg acgttcgata acgcccggag
    75541 cgattgcttc tacagcagag aagccgtcgt gatccagcgg acctttaccg tcgattggtg
    75601 cacccagagt gttaaccaca cggcccagca ggccacggcc aaccggaact tccaggatac
    75661 ggccagtaca cttaactttc atgccttcgg caaggtcagc atacggaccc ataacaaccg
    75721 caccaacaga gtcgcgctcg aggttcagtg cgatagcgta acggttaccc ggcagggaga
    75781 tcatttcacc ctgcatacaa tcggccaggc cgtgaatgcg gataacaccg tcacttacag
    75841 aaacaatagt accttcgttg tgagcttcac tcacaacatt gaactgagca atgcgctgct
    75901 tgatcagttc gctgatttcg gtggaattca gttgcatgct ccagtcccct taagactgca
    75961 agacgtctgc aaggcgctca agacgaccgc gtacgctgcc atcaatgacc atatcacccg
    76021 ctcggatgat aacgcctgcc attacagact tatcgatttt gcaattcagc ttaactttgc
    76081 gtgacagacg tttttccatc gcagcagaaa ttttcgcgag ctgttgttca ctcagtgcgg
    76141 cagcggaaat gacgtctacc tcagcggtag cctcactcac ggcacgcagg tgaataaact
    76201 gctccagaac atccgggagc gcgttaagac gaccattttc agccattacc cgaatcaggt
    76261 tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat aaacgactcg gcgagcgttt
    76321 ctggcgcaag cgcgccagag agaagctctg ccatttgttc gtttttggtt acctcggcgg
    76381 caaacgccag catgtcctgc cagcgttcta cactttggtg ttcgacggca aagtcaaaag
    76441 ctgctttggc gtaggggcga gctaccgtaa taaattcaga catcagcccc tccctcctta
    76501 cagttcagcg acaagtttat ccacgatgtc gctgttagca gcttcatcca cggaacgttc
    76561 gatgatcttc tcggcgccag caacagccag gatagcaact tgcttacgca gctcttcacg
    76621 ggcacgttta cgctcggctt caatttccgc ctgcgcctgg gccacgattt tagtacgttc
    76681 ctgttctgcc tcagctttcg cttcgtccag aatctgcgag cggcgtttgt tcgcctgctc
    76741 gatgattacc tgggcttccg ctttcgcttt tttcagctgg tcggtcgcgc tggcctttgc
    76801 aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg ccgtcagcaa tttctttttg
    76861 acgtttttcg atggctgcca ttaatggcgg ccatacgtac ttcatgcaga acagaacgaa
    76921 caggacaaac gcgatggcct ggccgaggat tgttgcgtta agattcacag cacaatgcct
    76981 ctatttagtt aacgttctga tattgctctt taaataaaag caacgcttac tacgcgacag
    77041 cgaacatcac gtacagaccc agacctacag cgatcatcgg gatagcatcc accagaccca
    77101 taacgataaa gaactgagta cgcagcagag gaatcagatc aggttgacgc gctgcgcctt
    77161 ccaggaattt acccccgagg atgccgatac cgatcgcagc accgattgcc gccagaccca
    77221 tcatcacagc ggcagccatg tacagcagat ccatattcag gttttccatg acagtctcca
    77281 gtttgtttca gttaaaacgt agtagtgttg gtaaattaat gttcttcaga cgccatcgac
    77341 agatagacga tcgtcagaac catgaagatg aaggcttgca gcgtaatgat caggatgtgg
    77401 aaaatggccc acggcacatt caggatccac tgtgaccacc acggcaacag accagcaatc
    77461 agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca gtcgcaaacc aagtgaaact
    77521 ggtttggaca gcaggcttac cccttcaagg attaagttga caggaatgaa cgcccagtga
    77581 ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga tgcctttcat tttgatgctg
    77641 tagaacagaa tcaggataaa cacgcccaat gccatagaca gcgttacgtt cacgtccgca
    77701 gacggaacca cacgcagtgc aggcagaccc agtacatgtt cagcaatgta cggcagcagg
    77761 tcgataggca gtaaatccat caggttcatc aggaataccc agacgaagat cgtcagggcc
    77821 agcggagcaa tcagcttgct tttgccatgg tacatgtctt tcacgctacc attaacaaag
    77881 ccgatcacca gctcaatcgc ggtctgaaac ttacctggca caccgctggt cgcctttttg
    77941 gctacgctac ggaataaaac caggaacaac agacccagca ccaccgagaa gaacatggag
    78001 tcaatattga ttgtccagaa ggtggctggg gggttatgtg gatccaccag cgagaatgta
    78061 cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt aatcctgcgg cgtcatattt
    78121 tctgaagcca tgatgccttt taccctttgt tgttaattac agccggtgcc agtatctgaa
    78181 ccaccagcac caaaacccac gtaacgatca gcggcaagaa taccgccttt aaaaccgcca
    78241 acgccaccac cagtaacacc aacatcgcca gaactttgaa agcttcgcca aatgcgaatg
    78301 tccaggccac ccggcctttc gctggtgtat gcgcctggtg acgccaggca aatatcataa
    78361 acaaaacgtt aggcagaaag actgccaggc ccccgcttat tgcagagacg ccccagaagg
    78421 ggtctttgag gctgaacagc aatccacttg ctatcaccac cagtaactga acgagcagaa
    78481 gcttccgagc aacgtttcga ctcacgagcg acacagacat cacgtttttc actcctgctc
    78541 ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa ggcttagagt caagcatcaa
    78601 aaagcggtca aattatacgg tgcgcccccg tgatttcaaa caataagtag ccaaaaggtg
    78661 aataaatgtt taaatatttt tccagtgcat acaattgcga cttttctgct aaccctgttc
    78721 gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat aaagcgtgct tcagatcaca
    78781 tattgcgcat gttcgcgcac agcatattta tttacttggc aaatgatgtc tttgcaagtt
    78841 tatgatattt cagtctaaaa acagatactg ttttaataaa tgacatttac acaacaaaaa
    78901 ccacccattg acatttttaa taatgtttta acagccaatg atggttctta gcgccgattt
    78961 ttagcagact gatattttca ctaatgactt attttctgct taccaaaaaa agccacgtta
    79021 tcttgttgat gcaaaagagt gaacgtggcg ttaaatgtaa ccagttatat cagtagaaaa
    79081 cctggttgtt gttaacagtc taaccggtca attttttatg atttttttga taaaaattaa
    79141 attttatttg ctttaatcac caccagatga cgttcgccat ccagggctgg aacctgaagt
    79201 ttaaccactg attcgacctg atattcttcg ggcaacaaag cgatttcatc ttccggcatt
    79261 tgccctttca gcgcgtagaa acggccttgc tcaccaggaa gatggtggca ccagctcacc
    79321 atatcgttca gagaggcaaa agcgcggcta attacgccat caaatggcgg ctctgaagga
    79381 aactcttcta ccctgctctg tactggttca atattctcca gtttaagctc atgttgcacc
    79441 tgacgaagga aacgcacacg tttaccaagg ctatccaaca gagtgaaatg ggcttcagga
    79501 cgcacgatag agagtggaat gcctggcagt cctggtccgg tgccgacatc gataaaccgt
    79561 tcaccttgca gatacggtgc caccacaatg ctatcgagaa tatggcgtac cagcatctca
    79621 ttaggatcgc ggaccgaagt caggttgtac gctttgttcc atttatgcag catattcacg
    79681 taggcaataa gctggttttt ctggtgatcg gtaagcgaaa tacctgcgtc tttcagcagt
    79741 aaggagagtt tgttgagcac ggtgattacc tgttcttgat gcgttgcctg gtaagcgggt
    79801 gcttaccagg catttttaat gcgttatgcg ctacgacgca gcataccctg ttttttcagc
    79861 cacaccagca gaatggagat ggccgcaggc gtgacgccag aaatacgtga agcctggccg
    79921 atagaggccg gtttgtgatc gttaagtttg gcgatcactt cgttagaaag accggatacc
    79981 tggcggtaat ccagtgtcgc gggtagcagg gtgttctcgt tacgcagctg cttttcgatc
    80041 tcatcttgct ggcgcgcgat ataaccttcg tatttaacct gaatctcaac ctgttccgcc
    80101 gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg tggttaattt ttcataagtc
    80161 atttccggac gacgcagcag atcttcacca ctggcttcac gggaaagcgg cgcagtcagg
    80221 tgagcattca cttcggctgc agcttccgcc gacggggtta cccaggtcga tttcagacgc
    80281 tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc gcgcccaacg ttcgtcatcc
    80341 accaggccca gttcacgacc gatttcagtc aaacgcagat ccgcattatc ttcgcgtagc
    80401 atcagacgat attctgcgcg cgaagtaaac atacgatacg gttctttggt tcctaaagtg
    80461 cacaggtcat caactagtac gccgagatac gcctgagaac gtgccggagc ccaaccttct
    80521 ttgtcagcag acagacgggc agcgttaaga ccggccagca aaccttgcgc agcggcttct
    80581 tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca gcccctggat aaacttgctc
    80641 tccagcgtcg gtttcaggtc gcgaggatcg aagaagtcat actcaatggc ataacccgga
    80701 cgcacgatct tcgcgttttc catcccctgc atagagcgga cgatttgcat ctgcacatcg
    80761 aacggcaggc tggtggagat accgttcgga taaatttcat tagaggtcag tccttccggt
    80821 tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca tgactttgtc ttcgatcgac
    80881 gggcagtagc gtgggccgac accttcgatc acccctgcat acattgggct acgatcgagg
    80941 ttactgcgga tcacatcatg ggttttctcg ttggtatgag tgatataaca cggcacctgc
    81001 tggggatgct gggacgcatt gcccataaac gagaataccg gcattgggtt atcgccatgc
    81061 tgttgcgcca gcacactaaa gtcgatagtt cgagcatcaa tacgcggtgg tgtcccggtt
    81121 ttcagacgac caacgcgcag cggcagttca cgcaaacggc gagaaagcgg aatggacggc
    81181 ggatcaccag cacggccacc gctgtaatta tccagaccga tatgaatttt accgtcgagg
    81241 aacgtcccaa cggtgagcac gacggctttg gcacggaact tcagtcccat ttgggtaaca
    81301 gcaccgacca cgcgatcgtt ttcgacaata agatcttcaa ccgcctgctg gaagatcatc
    81361 aggttcggtt ggttctccag cgccgtacgt accgcctgac ggtagagcac acgatccgcc
    81421 tgagctcggg tagcgcgaac cgccggtcct ttgcttgcgt ttagtatcct aaactggata
    81481 cccgcttgat cgatcgcttt cgccatcaga ccgccgagtg catccacttc ttttaccaga
    81541 tgtcccttcc caataccgcc gatcgccggg ttgcagctca tctgccccag agtgtcgata
    81601 ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg cggccatcgc ggcctcggtg
    81661 cctgcatgac ccccgccaat gatgatgacg tcaaaaggat ccggataaaa catggtgatt
    81721 gcctcgcata acgcggtatg aaaatggatt gaagcccggg ccgtggattc tactcaactt
    81781 tgtcggcttg agaaagacct gggatcctgg gtattaaaaa gaagatctat ttatttagag
    81841 atctgttcta ttgtgatctc ttattaggat cgcactgccc tgtggataac aaggatccgg
    81901 cttttaagat caacaacctg gaaaggatca ttaactgtga atgatcggtg atcctggacc
    81961 gtataagctg ggatcagaat gaggggttat acacaactca aaaactgaac aaccgttgtt
    82021 ccttggataa ctaccggttg atccaagctt cctgacagag ttatccacag tagatcgcac
    82081 gatctgtata cttatttgag taaattaatc cacgatccca gccattcttc tgccggatct
    82141 tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt cgcctgtctg ttttgcaccg
    82201 gaatttttga gttctgcctc gagtttatcg atagccccac aaaaggtgtc atattcacga
    82261 ctaccaatac cgattgcgcc aaagcggacc gcagaaagat cgggcttctg ttcctgcaat
    82321 gcttcataga aaggagaaag gttgtccgga atatctccgg caccgtgggt ggagctgata
    82381 atcagccaga tccccgaggc agataaatct tccaacagcg gaccgtgcag cgtttcggtg
    82441 gtaaaacccg cctcttccag cttttcagcc aggtgttctg ctacatattc ggcaccgccg
    82501 agggtgctgc cgctgataag agtgatatct gccataaacc gccaccttta ttaagagtgg
    82561 cgtattgtac gctgtgaacg cgttgggatc tacctgtgga aaagtatggg attaaaaaag
    82621 ccgatcaggg cttgatagta cgcatgatcg ggttttgcag gacgatcaat gtctcggtgg
    82681 actgaatttc atcaattgtt tggatcttgt tgataagtac atgctggaga gcgtcgatcg
    82741 aacggcacat tacttttata aagatgctgt agtggcctgt tgtgtaatag gcttcagtga
    82801 cttcatcaag gctttccagc tttgccagcg cggaagggta gtctttggcg ctctttaata
    82861 taatgccgat aaagcagcct acgtcataac cgagctgctt cgggctgaca tcaatacgag
    82921 ccccggtaat gatccccgcc tgcttcattt tctctactcg aacgtgaatc gtccccggac
    82981 tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg cgcattgccc attaatgctt
    83041 ccaggatgcc acggtccaga ttgtcgatca gataattttc cataggattt tcttatgcag
    83101 attgatgatt cattctattt tagccttctt ttttaatgaa tcaaaagtgt gttaggcttt
    83161 ttattgaatg attattgcat gtgtgtcggt ttttgttgct taatcataag caacaggacg
    83221 caggagtata aaaaatgaaa accgcttaca ttgccaaaca acgccaaatt agcttcgtga
    83281 aatctcactt ttctcgccaa ctggaagaac gtctggggct gatcgaagtc caggcgccga
    83341 ttcttagccg tgtgggggat ggcacgcagg ataacttgtc gggctgtgaa aaagcggtgc
    83401 aggtaaaagt gaaagctctg cctgatgccc agttcgaagt ggttcattca ctggcgaagt
    83461 ggaaacgtca gaccttaggg caacacgact tcagcgcggg cgaagggctg tacacgcaca
    83521 tgaaagccct tcgccccgat gaagaccgtc tttctccgtt gcactcggtc tatgttgacc
    83581 agtgggactg ggaacgcgta atgggcgacg gtgagcgtca attctcgact ctgaaaagca
    83641 cggtagaggc gatctgggcg ggaattaaag caaccgaagc tgcggttagc gaagagtttg
    83701 gcctggcacc gttcctgccg gatcagatcc acttcgtaca cagccaggag ttactgtctc
    83761 gttatccgga tctcgatgcc aaagggcgtg agcgggcgat agcgaaagat cttggcgcgg
    83821 tattccttgt cgggattggc ggcaagctga gcgatggtca tcgccacgac gtgcgcgcac
    83881 cggattatga tgactggagc accccgtcag agctgggtca tgcgggtctg aacggcgata
    83941 ttctggtgtg gaaccctgta ctggaagatg cgtttgagct ttcttccatg gggatccgcg
    84001 tggatgccga cacgttgaag catcagttgg cgctgaccgg tgatgaagat cgtctgcagc
    84061 tggaatggca tcaggcgctg ctgcgcggtg aaatgccgca gaccatcggc ggcggtatcg
    84121 gccagtctcg tttgaccatg ctgctgctgc aactgccgca tatcggccag gttcagtgtg
    84181 gtgtatggcc agctgctgtt cgcgagaacg tcccttctct gctgtaataa tttatcgccg
    84241 ccagcgtctg agcaggcggc ttcgcatccc ggtatcaaag cgccagatat gatcgaaaat
    84301 gcgcatgatg ccgggtttgc cgtgtgccga catcgccacg gcatgaaagc gatgctgatg
    84361 tacccgctgc agctctttca ctttactcgt cacgtcgtca ggcaaccgct gggcgataaa
    84421 atcagaaatc accaccgcat cggcatcaaa ccattccctg ctttgcaagc gttccataat
    84481 ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac tgctggctta aaaaacggat
    84541 tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg acgatctcgg tggaaaatag
    84601 cataatataa cagcgccggt tttcagcgag agcaatgcgc atcaaagcca ggcagaacgc
    84661 tttcgcacac tgttcattaa agccgcccat tgagccggaa gtatccacgc agacaataaa
    84721 cggtccgcgc ggctgttcgt cgtaatcttt atgcaccacc gggcgttcga tcactttttc
    84781 acgccacgac tcaccgtgca ggcgataggt gagcaactgt ttttccacca gccgacggta
    84841 aaactcatac tccagttccg ttatccctag tgtcgccagt tctggcggca gaagacgtaa
    84901 aatatcatcg ctttgttgca gaccatcaac ctgctcagga accgtcgccg gttcgcgcac
    84961 catggtgcgg aaggtttcca tctgcgcatc gttacgcggt attgatttgg cttcccgaga
    85021 acgccccagc tgctctgcca ggcgtttcag ttccggctgt tcgttaagaa attcaccgta
    85081 tttcacaatc aactgatagt cgccacgttt aagctgaccg gcgctcatat cccacagacg
    85141 accagctgcg gtattgttat ctgcgagaat cggttcaagt tgtccgctca gcgtcatgcg
    85201 ttcctgaact tcactcaaca gttgttcgcg ttcttcttct aatagctgtt gatttaacgt
    85261 cgttgcttgc acaatcagac ttaaacgcca gcgctggaga aaaagcgtgt gtaacgctga
    85321 agtgatcgcg ctgttagcat caaccaactg tcgggcttgt tctgcccatg gggaattcag
    85381 acgatgcagt aagtccagga tctgtggtag ctgcacaata aactgcggcg tggagaggag
    85441 ctggctttgc tgatagcaca tcacctcttc ggtgagttcc ggcgggactc gggcatcttt
    85501 cagccgactg cgcagcgcct cacgccagcg gggaacatca tcagtgattg ccgccttcag
    85561 tcgtgggaat ttttcaaaga agactgccag ctgcggtgag gccagcagcg cgatgatcat
    85621 ctcttcgatc aatccctctt cgctgacggc cagcatcaca ttaagcgtat ccagcgttag
    85681 cattgttgtg cctggcgaat ctgtgcaccg acatcctgta ggctggcttc aatgcgacct
    85741 aaccagtcgc cagggataaa cagacatttt tgctgttcgc tgaataaagc gtgttgcttg
    85801 cgccagtcgc tttcaagctc ttccagttgt tgttttattt caccaggcag accttcagcc
    85861 gatgaaccgg gaagtgccag cgtactgcct tgtaaactta catcgcgaac gacaagatgt
    85921 tgggcgctat caacttccag attcagtttc tgggcaaagc cgataccgtt cagtttaccg
    85981 cgaatttcac cacctttgct cagccactgt tccagcgcgc tacgctcaaa ggagatatga
    86041 accacttcca tatcatgcag ttttaacggt ttttgcagca gtagagtcag agtggaagca
    86101 gtaacgttaa caggaagttg atactgctga cgacggctga aaatgccgcc cagacgaatt
    86161 accgttaagg ctgttttatc gctttgttgc tgctggagtt gcaggtgacg ttgcacaatc
    86221 gcgccaaggc gagtcaacat cccttgctgt tgccaggcgt gaccggtcat caatacatca
    86281 atttgttgtt gtatcaaatt caggctttgc gcgtcatacc acaggcaatc tttcagcaaa
    86341 atgagatcta ccggggcaac agcactgcga ccgctaaaaa aggcgctggc ctgtaataat
    86401 cggatcgctt ttttccagcg acgatccgag acataaggcg catccggtaa tttatccagt
    86461 tgctggcgca gcataaaaat cagctcaaat acatgatcgg gcagcgtaat ttcaccaatc
    86521 tctttctgcc agcgttcata ttcttcatct gtgacctgca aggcatcagg aaccggattg
    86581 tcgttttcat cctgttgact ggtcagcatg gagcggaaat tcgctttatc ctgcacttta
    86641 tctaaccaca gacgaatcag catgcggtca tataacgctt ccagactgct gtctgcttcc
    86701 ggcagctcgt tggaggccgc caccagcagg cgcatcggga ttttttctac gtgtgcaccg
    86761 ttgcggaact ggcgctcgtt aatggcggtg agcaaggtat taagaattgc cgggcccgct
    86821 ttccagatct catccagaaa gacaatttct gcttccggca ggtaaccgct ggttaaacgt
    86881 tcatagcgcc cttcatcttt tagcgcctga atagaaaggg gaccaaaaac ttcttccggc
    86941 gtggagaagc gggtcatcag atattcaaac gcgcgggcat tctgaaaggc gaattttaag
    87001 cgccgggcga tcaaactttt ggcaatacct ggcgggccaa ggaggaacac actttcacca
    87061 cttaatgccg ctaataaaca caagcggatg gcgtggctac gttcataaag ccccttttcc
    87121 aacgaactgc tcaggcggga aattctttcc gctaataaat gagggtgagc cataatgaag
    87181 tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta tagacgtttc ttgctggtgg
    87241 ctaaaatagt ctcaaagggg gggtattttt ctttgagcca ggttaatgtg gccgcattta
    87301 ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt ttttgtgggc caagggacta
    87361 agcacacatt tcatatttca acgaaagact agtctatgag cactgataat aagcaatcat
    87421 tgcccgcgat taccctcgcg gcgattggag ttgtctacgg cgatattggt accagcccgt
    87481 tatatacact tcgtgaatgt ttgtccggcc agtttggttt tggcgttgaa cgcgatgccg
    87541 tgtttggctt tttatcgctg atcttctggc tgctaatctt tgtggtttcc attaaatatc
    87601 tcaccttcgt gatgcgggca gataacgccg gtgagggggg gatcctgacg ttgatgtcgc
    87661 ttgccgggcg taatacgtcg gcgcgaacca catcaatgct ggtgattatg gggctaatcg
    87721 gcggcagctt tttctatggt gaagtcgtca taacacccgc tatttcggtg atgtcagcca
    87781 ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg gatagttccc ctctcaatta
    87841 tcgttctcac tttattattt atgattcaaa agcatggcac ggctatggtc ggtaagctgt
    87901 ttgcgccgat catgctgacc tggtttttga ttctggcagg actggggtta cgtagcatta
    87961 ttgctaaccc ggaagtgctg catgcgctga atccgatgtg ggcggtgcat ttcttccttg
    88021 aatacaaaac ggtttctttt attgcattag gggcagtggt gctgtcgatt acgggggtcg
    88081 aggcgctgta tgctgatatg ggacactttg gtaagttccc tattcgtctg gcgtggttca
    88141 ctgtcgtatt accttcctta actcttaatt acttcggcca gggagcgctg ttgttaaaga
    88201 acccggaagc gattaagaac ccgttcttcc ttttggcacc ggactgggcg ctgatcccgc
    88261 tgctgatcat cgccgcactg gcgacggtaa tcgcctcgca ggcggttatc tctggcgtct
    88321 tttcattgac gcgtcaggcg gtacgtctgg gatatttgtc gccgatgcgc attattcaca
    88381 cctccgaaat ggagtcaggg caaatctata ttccgtttgt gaactggatg ctctatgtcg
    88441 cggtcgtgat tgtgattgtc agctttgagc actccagcaa cctggcggcg gcgtacggga
    88501 ttgcggtgac cggaaccatg gtgctgacgt ctattctctc gactaccgtg gcacgtcaga
    88561 actggcactg gaataagtat tttgttgcgc tgatcctgat tgctttcctt tgtgtcgata
    88621 ttccattgtt caccgctaac ctcgataaac tgctctccgg cggctggttg ccattgagcc
    88681 tcggtactgt gatgtttatc gtgatgacca cctggaagag cgagcgtttc cgcttgctgc
    88741 ggcggatgca tgaacatggt aactctctgg aagcgatgat tgcttcgctg gagaaatcac
    88801 cgcctgttcg cgtgcccggg accgcggtgt atatgtcgcg tgcaatcaac gtcattccct
    88861 ttgcgctgat gcataacctt aaacataaca aggtattgca tgagcgggtg attctgttaa
    88921 ctctgcgtac cgaagacgcg ccatatgtcc ataatgtccg tcgggtacag attgaacaac
    88981 tgtcgcccac tttctggcgc gtggtggcaa gttatggttg gcgagaaacg ccaaacgtgg
    89041 aagaagtttt ccaccgctgc ggtctggaag gattaagttg ccggatgatg gaaacctcct
    89101 tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc gtggtatttg cgtctgcgcg
    89161 gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc accagatcaa tttgaaattc
    89221 cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat ctaacgccag acgcctcctt
    89281 tctccataag ggggcgtttt tgttttcatg gttaatcacc atgtaaaacg tttcgaggtt
    89341 gatcacattt ccgtaacgtc acgatggttt tcccaactca gtcaggatta aactgcgggt
    89401 cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag gcaccgttct taattctgat
    89461 atttcatcgg tgatctcccg tctgggacat accgatacgc tggtggtgtg tgatgctggt
    89521 ttacccatcc ccaaaagtac aacgcgtatc gatatggcat taacccaggg tgtaccttct
    89581 tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg tcgaggctgc cattatcgcg
    89641 gaagagatca aacaacataa tccgcaactc cacgaaacgt tgctcactca ccttgagcag
    89701 ctgcaaaaac accagggaaa taccattgaa attcgttaca ccacgcatga acaattcaaa
    89761 caacaaaccg cagaaagtca ggcggtaatt cgcagcggag aatgttctcc gtatgcgaat
    89821 atcattctct gtgctggcgt gacgttctga ggccgtcatg gaagcattac ttcagcttaa
    89881 aggcatcgat aaagccttcc cgggcgtaaa agccctctcg ggcgcagcgt taaatgtcta
    89941 tccgggccgc gtgatggcgc tggtgggcga aaacggcgcg ggtaaatcca ccatgatgaa
    90001 agtgcttact ggtatctatg ctcgcgatgc cggcacgctt ttatggctgg ggaaagaaac
    90061 gacatttacc gggccgaaat cttcccagga agccgggatt gggattatcc atcaggaact
    90121 gaacctgatc ccgcagttga ccattgccga aaacattttc ctcggtcgtg agtttgttaa
    90181 tcgctttggc aaaattgact ggaaaaccat gtatgccgaa gcggataaat tgctggctaa
    90241 acttaacctg cgctttaaaa gcgacaagct ggtgggcgat ctttccatcg gtgaccagca
    90301 aatggttgaa atcgccaaag tgctgagctt tgagtcgaaa gtcatcatta tggatgaacc
    90361 gaccgatgcg ctgaccgata ccgaaaccga atccctgttc cgcgtcatcc gcgagctgaa
    90421 atcgcaaggc cgcggtattg tctatatctc ccaccgcatg aaagaaatct tcgagatttg
    90481 cgatgacgtt accgtttttc gtgatgggca atttattgct gagcgcgaag tggcatcact
    90541 gaccgaagat tcgctgattg agatgatggt gggtcgcaag ctggaagatc aatatccgca
    90601 cctggacaaa gcgccgggag atatccgcct gaaagtcgat aatctctgcg gacctggcgt
    90661 taacgatgtc tcttttactt tacgcaaagg cgaaattctt ggcgtctctg gtttgatggg
    90721 cgcaggtcgt accgaactga tgaaagtgct ctacggcgca ctaccgcgca ccagcggtta
    90781 cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg caggatggcc tggcaaacgg
    90841 cattgtgtat atctccgaag accgtaaacg tgacggttta gtgttgggca tgtcagtaaa
    90901 agagaacatg tcgctgacag cgctgcgcta cttcagccgc gctggcggca gtttgaagca
    90961 tgccgatgaa cagcaggctg tgagtgattt cattcgtctg tttaatgtga aaactccgtc
    91021 gatggaacag gcaattggtc tgctttccgg tggcaatcag caaaaagtgg cgattgcccg
    91081 cggtctgatg acacgcccca aagtgttgat ccttgatgaa cctacccgtg gcgtagatgt
    91141 cggcgcgaaa aaagagatct atcaactgat taaccagttc aaagccgatg gcttgagcat
    91201 cattctggtg tcatcggaga tgccagaagt attaggcatg agcgatcgca tcatcgtcat
    91261 gcatgaaggg catctcagcg gggaatttac tcgtgagcag gccacccagg aagtgttaat
    91321 ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag taaaaaaatg acaacccaga
    91381 ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat ggagcagaaa tcgcttatcg
    91441 ctctgctggt gctgatcgcg attgtctcga cgttaagccc gaactttttc accatcaata
    91501 acttattcaa tattctccag caaacctcag tgaacgccat tatggcggtc gggatgacgc
    91561 tggtgatcct gacgtcgggc atcgacttat cggtaggttc tctgttggcg ctgaccggcg
    91621 cagttgctgc atctatcgtc ggcattgaag tcaatgcgct ggtggctgtc gctgctgctc
    91681 tcgcgttagg tgccgcaatt ggtgcggtaa ccggggtgat tgtagcgaaa ggtcgcgtcc
    91741 aggcgtttat cgctacgctg gttatgatgc ttttactgcg cggcgtgacc atggtttata
    91801 ccaacggtag cccagtgaat accggcttta ctgagaacgc cgatctgttt ggctggtttg
    91861 gtattggtcg tccgctgggc gtaccgacgc cagtctggat catggggatt gtcttcctcg
    91921 cggcctggta catgctgcat cacacgcgtc tggggcgtta catctacgcg ctgggcggca
    91981 acgaagcggc aacgcgtctt tctggtatca acgtcaataa aatcaaaatc atcgtctatt
    92041 ctctttgtgg tctgctggca tcgctggccg ggatcattga agtggcgcgt ctctcctccg
    92101 cacaacccac ggcggggact ggctatgagc tggatgctat tgctgcggtg gttctgggcg
    92161 gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac gttgatcggc gcattaattc
    92221 ttggcttcct taataatgga ttgaatttgt taggtgtttc ctcctattac cagatgatcg
    92281 tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa caaaaagcag taataacgac
    92341 tacaggacat cttgaatatg aacatgaaaa aactggctac cctggtttcc gctgttgcgc
    92401 taagcgccac cgtcagtgcg aatgcgatgg caaaagacac catcgcgctg gtggtctcca
    92461 cgcttaacaa cccgttcttt gtatcgctga aagatggcgc gcagaaagag gcggataaac
    92521 ttggctataa cctggtggtg ctggactccc agaacaaccc ggcgaaagag ctggcgaacg
    92581 tgcaggactt aaccgttcgc ggcacaaaaa ttctgctgat taacccgacc gactccgacg
    92641 cagtgggtaa tgctgtgaag atggctaacc aggcgaacat cccggttatc actcttgacc
    92701 gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc tgataacgta ctgggcggca
    92761 aaatcgctgg tgattacatc gcgaagaaag cgggtgaagg tgccaaagtt atcgagctgc
    92821 aaggcattgc tggtacatcc gcagcccgtg aacgtggcga aggcttccag caggccgttg
    92881 ctgctcacaa gtttaatgtt cttgccagcc agccagcaga ttttgatcgc actaaaggtt
    92941 tgaacgtaat gcagaacctg ttgaccgctc atccggatgt tcaggctgta ttcgcgcaga
    93001 atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac tgccggtaaa tcggatgtga
    93061 tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc ggtgaatgat ggcaaactag
    93121 cagcgactat cgctcagcta cccgatcaga ttggcgcgaa aggcgtcgaa accgcagata
    93181 aagtgctgaa aggcgagaaa gttcaggcta agtatccggt tgatctgaaa ctggttgtta
    93241 agcagtagtt ttaatcaggt tgtatgacct gatggtgaca taaatacgtc atcgacagat
    93301 gaacgtgtaa tataaagaaa agcagggcac gcgccaccct aacacggtgg cgcattttat
    93361 ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt cttggcagca ttaatgctga
    93421 ccacattctt aatcttcaat cttttcctac tccaggcgaa accgtaaccg gtaaccacta
    93481 tcaggttgca tttggcggca aaggcgcgaa tcaggctgtg gctgctgggc gtagcggtgc
    93541 gaatatcgcg tttattgcct gtacgggtga tgacagcatt ggtgagagcg ttcgccagca
    93601 gctcgccact gataacattg atattactcc ggtcagcgtg atcaaaggcg aatcaacagg
    93661 tgtggcgctg atttttgtta atggcgaagg tgagaatgtc atcggtattc atgccggcgc
    93721 taatgctgcc ctttccccgg cgctggtgga agcgcaacgt gagcgtattg ccaacgcgtc
    93781 agcattatta atgcagctgg aatcaccact cgaaagtgtg atggcagcgg cgaaaatcgc
    93841 ccatcaaaat aagactatcg ttgcgcttaa cccggctccg gctcgcgaac ttcctgacga
    93901 actgctggcg ctggtggaca ttattacgcc aaacgaaacg gaagcagaaa agctcaccgg
    93961 tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg caggtactgc atgaaaaagg
    94021 tatccgtact gtactgatta ctttaggaag tcgtggtgta tgggctagcg tgaatggtga
    94081 aggtcagcgc gttcctggat tccgggtgca ggctgtcgat accattgctg ccggagatac
    94141 ctttaacggc gcgttaatca cggcgttgct ggaagaaaaa ccattgccag aggcgattcg
    94201 gtttgcccat gctgccgctg cgattgccgt aacacgtaaa ggcgcacaac cttccgtacc
    94261 gtggcgtgaa gagatcgacg catttttaga caggcagagg tgacgcttgg ctacaatgaa
    94321 agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt tctcacgtta tcaataaaga
    94381 tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg gcgattaaag aactcaatta
    94441 cgcgccatca gccctggcgc gtagcctcaa actcaatcaa acacatacca ttggcatgtt
    94501 gatcactgcc agtaccaatc ctttctattc agaactggtg cgtggcgttg aacgcagctg
    94561 cttcgaacgc ggttatagtc tcgtcctttg caataccgaa ggcgatgaac agcggatgaa
    94621 tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggt ttgctgttac tgtgcaccga
    94681 aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg acagtgccta ctgtgatgat
    94741 ggactgggct ccgttcgatg gcgacagcga tcttattcag gataactcgt tgctgggcgg
    94801 agacttagca acgcaatatc tgatcgataa aggtcatacc cgtatcgcct gtattaccgg
    94861 tccgctggat aaaactccgg cgcgcctgcg gttggaaggt tatcgggcgg cgatgaaacg
    94921 tgcgggtctc aacattcctg atggctatga agtcactggt gattttgaat ttaacggcgg
    94981 gtttgacgcc atgcgccaac tgttatcaca tccgctgcgt cctcaggccg tctttaccgg
    95041 aaatgacgct atggctgttg gcgtttacca ggcgttatat caggcagagt tacaggttcc
    95101 gcaggatatc gcggtgattg gctatgacga tatcgaactg gcaagcttta tgacgccacc
    95161 attaaccact atccaccaac cgaaagatga actgggggag ctggcgattg atgtactcat
    95221 ccatcggata acccagccga cccttcagca acaacgatta caacttactc cgattctgat
    95281 ggaacgcggt tcggcttaga tttacgctgt cttttgatca aattattacc atcggttgtt
    95341 ttcagaagca tgaacattgc tgctgaagca acagtaataa tgcccatcgt gataaacgta
    95401 tagtggaatt gttcgacagt cgttgtgcct tccatccctt cataaacgcg aaggacggcc
    95461 gcacttacag caacgcctaa actaatcgac agttgctgcg tgaccgccag aacactgtta
    95521 ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga tggtattcat cgcggtaaac
    95581 tgcgtcgaca tagccatccc taatataaac aacggcaaga tcagcatcca tatagccatt
    95641 gctggtgatt gcaaagagaa ctgagcgatc attagcccaa taatcaccgt gatccccact
    95701 aacgtatggc gatagcccag acgacgtaag acttgggtaa ccatcgattt tgcaataatg
    95761 gaacctaacg ctgtcggtgc catcatacag ccagcaataa acgcctgata accaaatcct
    95821 acctgtaaca tcaatggcat aaggaacggt acacagccgg tccccagacg ggttgcaata
    95881 ttgcctacga taccgatcga gaaagtgcgg gttttaaata aatctaatga aattaatggg
    95941 tttggcgtgc gtcgtgcatg gagaatatag agaagcagta acccgatgct ggtgacaatt
    96001 accgtcaagg caatccagct ggcgacaatc ttttccccga atagctctat tccgcttgag
    96061 aagagaacaa ggctgaggcc aaacagcaaa aagccagtga tatcgaatct gcgtcgtgcg
    96121 gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc ccgcaatacc tatggggata
    96181 ttgattaaaa atatccagtg ccaggttgcc caggtgacca gcacgccgcc aagaacgggg
    96241 cctaaaattg gccccaccag acccggcatg gcgacaaaat tcaatactgg aagaagttca
    96301 ttacgaggat aagcgcgcag taaggccagc cgagcaacag gcatcatcat tgcgccgcct
    96361 atcccctgaa taacccggaa gacaaccagc tgtggtagcg aattagaaag tgcgcaggcc
    96421 agagaaccca atgtgaacag actcacggca agggtaaaaa tgcgacgcgt accgaagcga
    96481 tcggctagcc atccgcttac cggaataagc atcgccaccg tcagcgtata actgatgatg
    96541 gctgattgca tcgcgagagg agaacgatta aggctatgag cgattgcggg taaggcggta
    96601 ttaagaatag tggcatcaag tgcctgcatg aagaaggcca tcgccgcgat ccacggcaaa
    96661 cccgccatac tgcgcttctt tttatcgctc attcaatgtc ctgttatcgg gttatcactt
    96721 atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc gcgtcgccat cgctttggat
    96781 aatcgcatcg acaatcgcct gatgcagatc cagctttatc actgtgtcgc tggtaattga
    96841 cgtgaagtaa gtgtgataaa ccgaatggaa tagcgaggcg aatgaggtca aaaacggatt
    96901 ggcgctcatt tcatagatat gctcatgcca ggccatatcg acttcgatcc agcgttcacg
    96961 gcgaaagttc tcttttaatg ccgccatttc ggccattaac gtattgagat gcgctttctg
    97021 ttccgcggtg ccaaccgttg ctgccagtag gcaggcttgc ggctccagac agatacgcat
    97081 aaccagaaag tgatcgatga cctgatgaaa gttctcttct gtcatccacc aggtaagcaa
    97141 ttcctgatca agaaaattcc agttcgattg tggcatgacc cgagtaccaa ttcgcggtcg
    97201 cggtaaaacc atcccttttg ccgttaacgt tttgaccgct tcgcgtaccg ctgtacgact
    97261 cactccaaat tgctcgccca gctcaatctc accgggcaaa atggtgccgg gttcatattc
    97321 accttttaag atccgttgcg ccagcttctc agccagaaca tacgaaaggt ttttctgtgc
    97381 agctaactgt tgtgcgctta aaggcattac ttatcttcct ttttcttttt attcctcctt
    97441 agtatgccac caggaagtgt gattacggtt gcaaaaacgg caaattgctt gttttatggc
    97501 acattaacgg ggcttttgct
//
LOCUS       DAZFZC010000004        93800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFZC010000004 DAZFZC010000000
VERSION     DAZFZC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.004"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..227
                     /locus_tag="ACHKXP_000310"
     CDS             <1..227
                     /locus_tag="ACHKXP_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645388.1"
                     /translation="AYLVVPQINYTMDLNIEDSSSEELLSDNTLEKDENSPDNGFEVG
                     EYNTYEAYNSEKQYFTREDYTYDYDLLNAI"
     gene            complement(841..1217)
                     /gene="rnpB"
                     /locus_tag="ACHKXP_000311"
     ncRNA           complement(841..1217)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKXP_000311"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1250..2395)
                     /gene="garK"
                     /locus_tag="ACHKXP_000312"
     CDS             complement(1250..2395)
                     /gene="garK"
                     /locus_tag="ACHKXP_000312"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO7645389.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCIIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2492..3382)
                     /gene="garR"
                     /locus_tag="ACHKXP_000313"
     CDS             complement(2492..3382)
                     /gene="garR"
                     /locus_tag="ACHKXP_000313"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7645390.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3412..4182)
                     /gene="garL"
                     /locus_tag="ACHKXP_000314"
     CDS             complement(3412..4182)
                     /gene="garL"
                     /locus_tag="ACHKXP_000314"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO7645391.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAELAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4198..5532)
                     /gene="garP"
                     /locus_tag="ACHKXP_000315"
     CDS             complement(4198..5532)
                     /gene="garP"
                     /locus_tag="ACHKXP_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO7645392.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5907..7478
                     /gene="garD"
                     /locus_tag="ACHKXP_000316"
     CDS             5907..7478
                     /gene="garD"
                     /locus_tag="ACHKXP_000316"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO7645393.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7627..7962
                     /gene="prlF"
                     /locus_tag="ACHKXP_000317"
     CDS             7627..7962
                     /gene="prlF"
                     /locus_tag="ACHKXP_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO7645394.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7962..8426
                     /gene="yhaV"
                     /locus_tag="ACHKXP_000318"
     CDS             7962..8426
                     /gene="yhaV"
                     /locus_tag="ACHKXP_000318"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO7645395.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8481..9290)
                     /gene="agaR"
                     /locus_tag="ACHKXP_000319"
     CDS             complement(8481..9290)
                     /gene="agaR"
                     /locus_tag="ACHKXP_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO7645396.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9539..10819
                     /gene="kbaZ"
                     /locus_tag="ACHKXP_000320"
     CDS             9539..10819
                     /gene="kbaZ"
                     /locus_tag="ACHKXP_000320"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO7645397.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10842..11315
                     /gene="agaV"
                     /locus_tag="ACHKXP_000321"
     CDS             10842..11315
                     /gene="agaV"
                     /locus_tag="ACHKXP_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO7645398.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11326..12105
                     /gene="agaW"
                     /locus_tag="ACHKXP_000322"
     CDS             11326..12105
                     /gene="agaW"
                     /locus_tag="ACHKXP_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO7645399.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            12095..12973
                     /gene="agaE"
                     /locus_tag="ACHKXP_000323"
     CDS             12095..12973
                     /gene="agaE"
                     /locus_tag="ACHKXP_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO7645400.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLARQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12991..13425
                     /gene="agaF"
                     /locus_tag="ACHKXP_000324"
     CDS             12991..13425
                     /gene="agaF"
                     /locus_tag="ACHKXP_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO7645401.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13422..14555
                     /gene="nagA"
                     /locus_tag="ACHKXP_000325"
     CDS             13422..14555
                     /gene="nagA"
                     /locus_tag="ACHKXP_000325"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7645402.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14906..16060
                     /gene="agaS"
                     /locus_tag="ACHKXP_000326"
     CDS             14906..16060
                     /gene="agaS"
                     /locus_tag="ACHKXP_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO7645403.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            16073..16933
                     /gene="kbaY"
                     /locus_tag="ACHKXP_000327"
     CDS             16073..16933
                     /gene="kbaY"
                     /locus_tag="ACHKXP_000327"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO7645404.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            17100..17576
                     /gene="agaB"
                     /locus_tag="ACHKXP_000328"
     CDS             17100..17576
                     /gene="agaB"
                     /locus_tag="ACHKXP_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO7645405.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKFYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17615..18418
                     /gene="agaC"
                     /locus_tag="ACHKXP_000329"
     CDS             17615..18418
                     /gene="agaC"
                     /locus_tag="ACHKXP_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO7645406.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVTSKNEEEDYSNGI"
     gene            18408..19199
                     /gene="agaD"
                     /locus_tag="ACHKXP_000330"
     CDS             18408..19199
                     /gene="agaD"
                     /locus_tag="ACHKXP_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO7645407.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDNTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            19200..19955
                     /gene="agaI"
                     /locus_tag="ACHKXP_000331"
     CDS             19200..19955
                     /gene="agaI"
                     /locus_tag="ACHKXP_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO7645408.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPNAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATDRFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            20356..20940
                     /gene="yraH"
                     /locus_tag="ACHKXP_000332"
     CDS             20356..20940
                     /gene="yraH"
                     /locus_tag="ACHKXP_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7645409.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRNIGERSPKIPFTIPLVNCPMTGWEYDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGIAATGVGIRIDDAESGNIMPLNAMGNDNTVYQIPAESNGI
                     VNVDLIAYYVSTVVPSEITPGEADAIVNVTLDYR"
     gene            21020..21715
                     /gene="yraI"
                     /locus_tag="ACHKXP_000333"
     CDS             21020..21715
                     /gene="yraI"
                     /locus_tag="ACHKXP_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7645410.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            21744..24260
                     /gene="yraJ"
                     /locus_tag="ACHKXP_000334"
     CDS             21744..24260
                     /gene="yraJ"
                     /locus_tag="ACHKXP_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7645411.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQQLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGVGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            24271..25362
                     /gene="yraK"
                     /locus_tag="ACHKXP_000335"
     CDS             24271..25362
                     /gene="yraK"
                     /locus_tag="ACHKXP_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7645412.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKNNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(25405..26265)
                     /gene="rsmI"
                     /locus_tag="ACHKXP_000336"
     CDS             complement(25405..26265)
                     /gene="rsmI"
                     /locus_tag="ACHKXP_000336"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO7645413.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCRESGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            26330..28366
                     /gene="lpoA"
                     /locus_tag="ACHKXP_000337"
     CDS             26330..28366
                     /gene="lpoA"
                     /locus_tag="ACHKXP_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO7645414.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            28324..28719
                     /gene="yraN"
                     /locus_tag="ACHKXP_000338"
     CDS             28324..28719
                     /gene="yraN"
                     /locus_tag="ACHKXP_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO7645415.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            28739..29329
                     /gene="diaA"
                     /locus_tag="ACHKXP_000339"
     CDS             28739..29329
                     /gene="diaA"
                     /locus_tag="ACHKXP_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO7645416.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            29339..29914
                     /gene="dolP"
                     /locus_tag="ACHKXP_000340"
     CDS             29339..29914
                     /gene="dolP"
                     /locus_tag="ACHKXP_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7645417.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(30028..31068)
                     /gene="yraQ"
                     /locus_tag="ACHKXP_000341"
     CDS             complement(30028..31068)
                     /gene="yraQ"
                     /locus_tag="ACHKXP_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7645418.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(31141..31776)
                     /gene="yraR"
                     /locus_tag="ACHKXP_000342"
     CDS             complement(31141..31776)
                     /gene="yraR"
                     /locus_tag="ACHKXP_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO7645419.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            31904..32422
                     /gene="yhbO"
                     /locus_tag="ACHKXP_000343"
     CDS             31904..32422
                     /gene="yhbO"
                     /locus_tag="ACHKXP_000343"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO7645420.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(32402..32845)
                     /gene="yhbP"
                     /locus_tag="ACHKXP_000344"
     CDS             complement(32402..32845)
                     /gene="yhbP"
                     /locus_tag="ACHKXP_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO7645421.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            32896..33198
                     /gene="yhbQ"
                     /locus_tag="ACHKXP_000345"
     CDS             32896..33198
                     /gene="yhbQ"
                     /locus_tag="ACHKXP_000345"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO7645422.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLVFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(33185..33688)
                     /gene="yhbS"
                     /locus_tag="ACHKXP_000346"
     CDS             complement(33185..33688)
                     /gene="yhbS"
                     /locus_tag="ACHKXP_000346"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7645423.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(33682..34206)
                     /gene="ubiT"
                     /locus_tag="ACHKXP_000347"
     CDS             complement(33682..34206)
                     /gene="ubiT"
                     /locus_tag="ACHKXP_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO7645424.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            34415..35410
                     /gene="ubiU"
                     /locus_tag="ACHKXP_000348"
     CDS             34415..35410
                     /gene="ubiU"
                     /locus_tag="ACHKXP_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO7645425.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTIGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            35419..36297
                     /gene="ubiV"
                     /locus_tag="ACHKXP_000349"
     CDS             35419..36297
                     /gene="ubiV"
                     /locus_tag="ACHKXP_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO7645426.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            36378..37385
                     /gene="yhbW"
                     /locus_tag="ACHKXP_000350"
     CDS             36378..37385
                     /gene="yhbW"
                     /locus_tag="ACHKXP_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO7645427.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(37503..38747)
                     /gene="mtr"
                     /locus_tag="ACHKXP_000351"
     CDS             complement(37503..38747)
                     /gene="mtr"
                     /locus_tag="ACHKXP_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO7645428.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(38901..40790)
                     /gene="deaD"
                     /locus_tag="ACHKXP_000352"
     CDS             complement(38901..40790)
                     /gene="deaD"
                     /locus_tag="ACHKXP_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO7645429.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRHFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(40783..40863)
                     /gene="yrbN"
                     /locus_tag="ACHKXP_000353"
     CDS             complement(40783..40863)
                     /gene="yrbN"
                     /locus_tag="ACHKXP_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO7645430.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(40970..41854)
                     /gene="nlpI"
                     /locus_tag="ACHKXP_000354"
     CDS             complement(40970..41854)
                     /gene="nlpI"
                     /locus_tag="ACHKXP_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO7645431.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(41963..44098)
                     /gene="pnp"
                     /locus_tag="ACHKXP_000355"
     CDS             complement(41963..44098)
                     /gene="pnp"
                     /locus_tag="ACHKXP_000355"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO7645432.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKLGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAQLLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(44345..44614)
                     /gene="rpsO"
                     /locus_tag="ACHKXP_000356"
     CDS             complement(44345..44614)
                     /gene="rpsO"
                     /locus_tag="ACHKXP_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO7645433.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(44763..45707)
                     /gene="truB"
                     /locus_tag="ACHKXP_000357"
     CDS             complement(44763..45707)
                     /gene="truB"
                     /locus_tag="ACHKXP_000357"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO7645434.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(45707..46105)
                     /gene="rbfA"
                     /locus_tag="ACHKXP_000358"
     CDS             complement(45707..46105)
                     /gene="rbfA"
                     /locus_tag="ACHKXP_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO7645435.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDEED"
     gene            complement(46269..48941)
                     /gene="infB"
                     /locus_tag="ACHKXP_000359"
     CDS             complement(46269..48941)
                     /gene="infB"
                     /locus_tag="ACHKXP_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO7645436.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(48966..50453)
                     /gene="nusA"
                     /locus_tag="ACHKXP_000360"
     CDS             complement(48966..50453)
                     /gene="nusA"
                     /locus_tag="ACHKXP_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO7645437.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(50481..50933)
                     /gene="rimP"
                     /locus_tag="ACHKXP_000361"
     CDS             complement(50481..50933)
                     /gene="rimP"
                     /locus_tag="ACHKXP_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO7645438.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(51140..51216)
                     /locus_tag="ACHKXP_000362"
     tRNA            complement(51140..51216)
                     /locus_tag="ACHKXP_000362"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51180..51182),aa:Met,seq:cat)
     gene            51564..52907
                     /gene="argG"
                     /locus_tag="ACHKXP_000363"
     CDS             51564..52907
                     /gene="argG"
                     /locus_tag="ACHKXP_000363"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO7645439.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(52915..54540)
                     /gene="yhbX"
                     /locus_tag="ACHKXP_000364"
     CDS             complement(52915..54540)
                     /gene="yhbX"
                     /locus_tag="ACHKXP_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7645440.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(54999..55085)
                     /locus_tag="ACHKXP_000365"
     tRNA            complement(54999..55085)
                     /locus_tag="ACHKXP_000365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55049..55051),aa:Leu,seq:gag)
     gene            complement(55100..55432)
                     /gene="secG"
                     /locus_tag="ACHKXP_000366"
     CDS             complement(55100..55432)
                     /gene="secG"
                     /locus_tag="ACHKXP_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO7645441.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(55660..56997)
                     /gene="glmM"
                     /locus_tag="ACHKXP_000367"
     CDS             complement(55660..56997)
                     /gene="glmM"
                     /locus_tag="ACHKXP_000367"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO7645442.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(56990..57838)
                     /gene="folP"
                     /locus_tag="ACHKXP_000368"
     CDS             complement(56990..57838)
                     /gene="folP"
                     /locus_tag="ACHKXP_000368"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO7645443.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(57928..59862)
                     /gene="ftsH"
                     /locus_tag="ACHKXP_000369"
     CDS             complement(57928..59862)
                     /gene="ftsH"
                     /locus_tag="ACHKXP_000369"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO7645444.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(59962..60591)
                     /gene="rlmE"
                     /locus_tag="ACHKXP_000370"
     CDS             complement(59962..60591)
                     /gene="rlmE"
                     /locus_tag="ACHKXP_000370"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO7645445.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            60717..61010
                     /gene="yhbY"
                     /locus_tag="ACHKXP_000371"
     CDS             60717..61010
                     /gene="yhbY"
                     /locus_tag="ACHKXP_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO7645446.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(61166..61642)
                     /gene="greA"
                     /locus_tag="ACHKXP_000372"
     CDS             complement(61166..61642)
                     /gene="greA"
                     /locus_tag="ACHKXP_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO7645447.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            61890..63323
                     /gene="dacB"
                     /locus_tag="ACHKXP_000373"
     CDS             61890..63323
                     /gene="dacB"
                     /locus_tag="ACHKXP_000373"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7645448.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            63378..63485
                     /locus_tag="ACHKXP_000374"
                     /pseudo
     CDS             63378..63485
                     /locus_tag="ACHKXP_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(63509..64681)
                     /gene="cgtA"
                     /locus_tag="ACHKXP_000375"
     CDS             complement(63509..64681)
                     /gene="cgtA"
                     /locus_tag="ACHKXP_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO7645449.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(64697..65662)
                     /gene="yhbE"
                     /locus_tag="ACHKXP_000376"
     CDS             complement(64697..65662)
                     /gene="yhbE"
                     /locus_tag="ACHKXP_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7645450.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(65789..66046)
                     /gene="rpmA"
                     /locus_tag="ACHKXP_000377"
     CDS             complement(65789..66046)
                     /gene="rpmA"
                     /locus_tag="ACHKXP_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO7645451.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(66067..66378)
                     /gene="rplU"
                     /locus_tag="ACHKXP_000378"
     CDS             complement(66067..66378)
                     /gene="rplU"
                     /locus_tag="ACHKXP_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO7645452.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            66637..67608
                     /gene="ispB"
                     /locus_tag="ACHKXP_000379"
     CDS             66637..67608
                     /gene="ispB"
                     /locus_tag="ACHKXP_000379"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO7645453.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            67837..68115
                     /gene="sfsB"
                     /locus_tag="ACHKXP_000380"
     CDS             67837..68115
                     /gene="sfsB"
                     /locus_tag="ACHKXP_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO7645454.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(68163..69422)
                     /gene="murA"
                     /locus_tag="ACHKXP_000381"
     CDS             complement(68163..69422)
                     /gene="murA"
                     /locus_tag="ACHKXP_000381"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO7645455.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(69477..69731)
                     /gene="ibaG"
                     /locus_tag="ACHKXP_000382"
     CDS             complement(69477..69731)
                     /gene="ibaG"
                     /locus_tag="ACHKXP_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO7645456.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(69891..70184)
                     /gene="mlaB"
                     /locus_tag="ACHKXP_000383"
     CDS             complement(69891..70184)
                     /gene="mlaB"
                     /locus_tag="ACHKXP_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO7645457.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(70184..70819)
                     /gene="mlaC"
                     /locus_tag="ACHKXP_000384"
     CDS             complement(70184..70819)
                     /gene="mlaC"
                     /locus_tag="ACHKXP_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO7645458.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(70838..71389)
                     /gene="mlaD"
                     /locus_tag="ACHKXP_000385"
     CDS             complement(70838..71389)
                     /gene="mlaD"
                     /locus_tag="ACHKXP_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO7645459.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(71394..72176)
                     /gene="mlaE"
                     /locus_tag="ACHKXP_000386"
     CDS             complement(71394..72176)
                     /gene="mlaE"
                     /locus_tag="ACHKXP_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO7645460.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(72184..72993)
                     /gene="mlaF"
                     /locus_tag="ACHKXP_000387"
     CDS             complement(72184..72993)
                     /gene="mlaF"
                     /locus_tag="ACHKXP_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO7645461.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            73203..74180
                     /gene="yrbG"
                     /locus_tag="ACHKXP_000388"
     CDS             73203..74180
                     /gene="yrbG"
                     /locus_tag="ACHKXP_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO7645462.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            74194..75180
                     /gene="kdsD"
                     /locus_tag="ACHKXP_000389"
     CDS             74194..75180
                     /gene="kdsD"
                     /locus_tag="ACHKXP_000389"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO7645463.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            75201..75767
                     /gene="kdsC"
                     /locus_tag="ACHKXP_000390"
     CDS             75201..75767
                     /gene="kdsC"
                     /locus_tag="ACHKXP_000390"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO7645464.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            75764..76339
                     /gene="lptC"
                     /locus_tag="ACHKXP_000391"
     CDS             75764..76339
                     /gene="lptC"
                     /locus_tag="ACHKXP_000391"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO7645465.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            76308..76865
                     /gene="lptA"
                     /locus_tag="ACHKXP_000392"
     CDS             76308..76865
                     /gene="lptA"
                     /locus_tag="ACHKXP_000392"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO7645466.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            76872..77597
                     /gene="lptB"
                     /locus_tag="ACHKXP_000393"
     CDS             76872..77597
                     /gene="lptB"
                     /locus_tag="ACHKXP_000393"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO7645467.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            77645..79078
                     /gene="rpoN"
                     /locus_tag="ACHKXP_000394"
     CDS             77645..79078
                     /gene="rpoN"
                     /locus_tag="ACHKXP_000394"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO7645468.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            79101..79388
                     /gene="hpf"
                     /locus_tag="ACHKXP_000395"
     CDS             79101..79388
                     /gene="hpf"
                     /locus_tag="ACHKXP_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO7645469.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            79506..79997
                     /gene="ptsN"
                     /locus_tag="ACHKXP_000396"
     CDS             79506..79997
                     /gene="ptsN"
                     /locus_tag="ACHKXP_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO7645470.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            80043..80897
                     /gene="rapZ"
                     /locus_tag="ACHKXP_000397"
     CDS             80043..80897
                     /gene="rapZ"
                     /locus_tag="ACHKXP_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO7645471.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            80894..81166
                     /gene="npr"
                     /locus_tag="ACHKXP_000398"
     CDS             80894..81166
                     /gene="npr"
                     /locus_tag="ACHKXP_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO7645472.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            81380..82012
                     /gene="yrbL"
                     /locus_tag="ACHKXP_000399"
     CDS             81380..82012
                     /gene="yrbL"
                     /locus_tag="ACHKXP_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO7645473.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(82009..82737)
                     /gene="mtgA"
                     /locus_tag="ACHKXP_000400"
     CDS             complement(82009..82737)
                     /gene="mtgA"
                     /locus_tag="ACHKXP_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO7645474.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRKMYQLGGEPFMQQHQLD"
     gene            complement(82734..83387)
                     /gene="elbB"
                     /locus_tag="ACHKXP_000401"
     CDS             complement(82734..83387)
                     /gene="elbB"
                     /locus_tag="ACHKXP_000401"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO7645475.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(83617..85953)
                     /gene="arcB"
                     /locus_tag="ACHKXP_000402"
     CDS             complement(83617..85953)
                     /gene="arcB"
                     /locus_tag="ACHKXP_000402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO7645476.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(86049..86978)
                     /gene="yhcC"
                     /locus_tag="ACHKXP_000403"
     CDS             complement(86049..86978)
                     /gene="yhcC"
                     /locus_tag="ACHKXP_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO7645477.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            87653..92113
                     /gene="gltB"
                     /locus_tag="ACHKXP_000404"
     CDS             87653..92113
                     /gene="gltB"
                     /locus_tag="ACHKXP_000404"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO7645478.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPTDLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            92126..93544
                     /gene="gltD"
                     /locus_tag="ACHKXP_000405"
     CDS             92126..93544
                     /gene="gltD"
                     /locus_tag="ACHKXP_000405"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO7645479.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
BASE COUNT        23102 a        25067 c        23303 g        22328 t
ORIGIN      
        1 atgcttatct ggtcgtcccc caaattaatt atactatgga tttaaacata gaagactcta
       61 gctcagaaga gttactttca gataataccc tcgagaaaga cgaaaattct ccggacaatg
      121 gctttgaggt cggggaatat aacacatatg aagcatataa ctcagagaag caatatttta
      181 ccagagagga ctatacgtat gattacgacc ttttaaatgc aatatagact taataacttc
      241 ttacaaagtc tattattaat accgtagata tttattcatc ttaccctgtg caccaaaagc
      301 aaaaactcac tgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct gatgcgctac
      361 gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt gtaggcagga
      421 taaggcgttc acgccgcatc cggcatgaat aaagcgcact caacaatctg aaacccgccg
      481 gaacggttta ttacgtacat caggtaaaac tgaccgataa gccgctttct tttgggtata
      541 gtgtcgtgga cagtcattca tctttctgcc cctccaaaag taaaaacccg ccgaagcggg
      601 tttttacgta aaacaggtga aactgaccga taagccgctt tcttttgggt atagtgtcgt
      661 ggacagtcat tcatctttct acccctccaa aagcaaaaac ccgccgaagc gggtttttac
      721 gtaaaccagg tgaaactgac cgataagccg ctttcttttg ggtatagcgt cgtggacagt
      781 cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt tacgtaaatc
      841 aggtgaaact gaccgataag ccgggttctg tcgtggacag tcattcatct aggccagcaa
      901 tcgctcactg gctcaagcag cctacccggg ttcagtacgg gccgtacctt atgaacccct
      961 atttggcctt gctccgggtg gagtttaccg tgccacggac tgttaccagc cgcgcggtgc
     1021 gctcttaccg caccctttca cccttacctg atcccgcttg cgcgggccat cggcggtttg
     1081 ctctctgttg cactggtcgt gggtttcccc cccaggcgtt acctggcacc ctgccctatg
     1141 gagcccggac tttcctcccc tccgcccgtc tcccccgaag aggacgacga cgaagcggcg
     1201 actgtctggt cagcttcggc gcgcagtata gagggtttgc gcgcccttgt caccccgcgt
     1261 tgcgcattcc aatcgccagt gtcgcggcga tattacgtga agcacggcag atattgtcat
     1321 aagccccgcg gaatgcttcg tccaacgtac ctatgctggt caatacgctg aagaccgcat
     1381 caatgccatg ctgatgtaca acgccaacat catcggtcag gctacccgca atgccaatca
     1441 ccggtttatg gtacttcttc gccacgtttg cgacaccaat cggtaccttc ccgtgaatac
     1501 tctggctgtc aatacgccct tcaccggtga tcaccagcgt acaatcgtga atatgttcct
     1561 ccagattcag cgccgtagtg acgatttcaa taccactttt cagttccgca ccaagaaacg
     1621 ccattagcgc cgcgcccata ccacccgcag ctcctgcacc ggggacatct ttcacatcaa
     1681 catgcagcgc ttttttaatg acctcggcat agtgagagag gttattgtcc agctcaacaa
     1741 tcatcgcttc actggctccc ttttgtgggc caaagatgcg cgatgcgccg ttatcgccca
     1801 ccagcggatt ggtgacatca caagcgacgc gaatgatgca atcttttaag cgcggatcga
     1861 ggccggaaat atcaatatca ttcagagtat taagactacc gccgccaaaa ccaatttcat
     1921 tgccgttggc gtcgcataat ttcgccccca gcgcctgtac catgcctgcg ccgccatcat
     1981 ttgtagcgct gccgccaatg ccgataataa tgtttgtcgc accgctctcc agcgcctgca
     2041 ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg atcgcgtttt tccgcaggta
     2101 ccagctccag cccactggcc gccgccattt caataaacgc ggttttgcca tcgccggaga
     2161 tcccccaact ggcattcact ttctcgccca gcggccctgt aacccaggcg tgacgttcag
     2221 ccccctgggt ggctgcaatc atcgcttcca ccgttccttc gccaccgtcg gcaaccggaa
     2281 cagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc tttttctatc gcctgcgcaa
     2341 cctcgctggc agataaactt tctttataag agtctggggc gattacgatt ttcataccta
     2401 tgcctgttac cacatgacgc cggagggcgt ttctcttatt cggcctggat tccaggcccg
     2461 gattgcaata cgccatccgg gcacgacgtc attaacgagt aacttcgact ttcgccagtt
     2521 tttcgtagta gcacgccagg gcgctatgat ccgccgttcc taaaccatct gctcgcagtg
     2581 cctgcatcat ctccataacc gcagctgtga gcggcagttg tgcgccgacg ccgtgagaag
     2641 tatccagcgc attcgccaga tccttaatat gcagatcaat acggaagccc ggcttgaagt
     2701 tgcggtccat caccatcggc gctttggcat ccagcacggt actgcccgcc agtccaccgc
     2761 gaattgcctg ataaaccagg tccgggttaa cgcccgcttt agttgccagc gttaacgctt
     2821 ctgacatcgc ggcaatattc agcgccacaa tgacctgatt tgccagtttg gtgacgttac
     2881 ctgcaccgat ttccccggta tgcaccacgg aacccgccat cgctttcatc aaatcatagt
     2941 atttgtcgaa aatagccttg tcgccgccca ccatcactga cagcgtaccg tcgatggctt
     3001 tcggttcacc gccgctcacc ggagcatcca gcatatcaat gcctttcgct ttcagcgctt
     3061 cgctgatttc acggcttgcc agcggtgcga tagaactcat atcgatcaat accgtacctg
     3121 gcttcgcgcc ttcaataatg ccattctcac ccagcgccac ctctttcaca tgaggggagt
     3181 ttggcagcat ggttatgatg acgtcgcact gttcagcgat cgctttagcc gtagacgctg
     3241 tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg gttacggtca gcaaccacca
     3301 gcgagtaacc tgctttcaga aggtttttac tcattggttt acccataatc cccaggccaa
     3361 taaaaccaac tttcatagtc atatcaatca tctctcttgt tgcggtggtg gttatttttt
     3421 aaaggtatca gccagtttct gagtggcaga gcggaagacg ccgagatcgc tgccgacagc
     3481 cacaaacgtc gcgccccatt ccagataacg acgcgcatcg gcttcgaccg gcgcgaggat
     3541 accgctgggt ttgccgtgcg cgctggcacg gttaaaaatg tgctgaattg ctttttgtac
     3601 atccgggtgt gatgcattgc cgagatggcc taatgccgcg gccagatcgc tggggccgac
     3661 gaagatgccg tctacgcctt cggtagcggc aatggcatcg acgttatcta cgccctgctg
     3721 actttctatc tggaccagaa tagtgatgtt cttgttcgac tgagcgaaat aatccgccac
     3781 ggtgccaaac atattggcgc ggtgagaaac ggagacgccg cgaatgcctt ccggtgggta
     3841 acgggttgat gccaccgcca gctctgcttc ctcttttgtt tctacaaaag gaatcaggaa
     3901 gttatagaaa ccgatatcca gaagacgctt aataattacc ggctcgttgg tcggcactcg
     3961 cactactggc gcgctggcgc tgcctttcaa ggccattaac tgcggaataa acgtggagat
     4021 atcgtttggc gcatgttcgc catccagcac cagccagtca aacccagcca aaccaagaac
     4081 ttcagtgcta atcgggttag agagtgctga ccagcaacca atttgtacct gtttcgcagc
     4141 cagtgcggct ttgaatttat tcgggaaaac atcgttattc atcgcttata cctttgctta
     4201 tttctgcaat tccatacgtt taatgtcgcc aactacgaag aggtagcaga ccatcgccat
     4261 cagcgctgaa catcccacga aaaccagtgc tgcattgaag gagtgcagtt cacttaccag
     4321 gtagccaatc accagtggag tgacaatgga ggcaacattg ccaaagacgt taaagacgcc
     4381 gccgcagagg ccaacaatct ctttcggcgc ggtgtcagaa atcaccggcc agcccagcgc
     4441 accaaatcct ttgccaaaga aagccagcgc catcagcatg accaccagcg tggtgttgtt
     4501 ggtgtagtta cataagatga tggtggaagc cagcaacatt cccagcacaa tcggtagctt
     4561 acgtgccagg gtcagggata aaccgcgttt gatcagataa tccgagaaga cacctcccag
     4621 cacgccgccc gcaaaaccac acagtgctgg aatcgaggcg accagaccca ctttcagaat
     4681 cgacatgcct ttttcctgca ccagataaat cgggaaccag gtgaggaaga accaggtgat
     4741 ggtgttgata aaatattgtc cgaaaaatac gcccagcatc atgcggttag agagcaattg
     4801 cttgatgtaa tgcagtttgg gtccgcttgc tgccgcactg cccggctttt tgtggtccat
     4861 atcgaccacc gcgccatttt cagagataaa cttcagctct tccgcagaca tacgtgggtg
     4921 atctgtcggg ttatgaatca acttgatcca cagcgccgtc agcacaaaac caatcacccc
     4981 cataacggta aagacgtgct cccagcccca ggcgaaagtc agccagccaa gcagcggcga
     5041 aaagagcgcc agcgagaaat attgcgccga gttaaagatg gcggaggcag taccacgttc
     5101 tttcgtcggg aaccaggcgg cgacaattcg ggcgttcgcc gggaatgatg gcgcttccga
     5161 gaagccgagc ataaagcgca taaagaacat ggagatccct gcccaggcca gcgggaacat
     5221 atcaacaaag ccttgcagga aggtgaatag cgaccagaaa aagaggctgt aggtgtaaac
     5281 ttttttcgag ccaaacttat caagcagcca gccgccgggg atttgcatca gcaagtaggc
     5341 ccagccaaaa gcggagaaga tgtaacccat cgaaaccgca cttaactgca actcttttgc
     5401 cacttcggta ccagcaatag acagcgttgc acgatcggcg tagttaacgg cggtaacaat
     5461 aaaaataatc agtaatatta aatagcgggt atgcacgcct ttcttttttt cgtcaacggt
     5521 gtccagaatc attttattta cctcgggtac ttatgctgat ttttattatt atggggaagg
     5581 tgttatttat gagtttcatt tatgccgtaa cgacaatgaa ctcgggaatt agtataagca
     5641 gcgcgagaat aataatcatt gtgcaaatgc taatttaatt aatactattt aaatattatt
     5701 ttgagcatat gcacataagg ttgcgcgcta aagcacagat ttgcgcttta ccttaccggg
     5761 cggcactgca atccctgaaa tgattgacat tgatcacatt tctgcgttta aactcctgac
     5821 attcttattt cacccaatga agtcatttat ttttaaatga gaccaggtcc tcattttaat
     5881 aacccctggc tggagaatat tgcacaatgg ccaacatcga aatcagacaa gaaacgccaa
     5941 ctgcgtttta tataaaagtt cacgacacag ataatgtggc aattattgtt aatgataatg
     6001 gcctgaaagc aggaacgcgt tttccggatg ggctggaatt aattgaacat attccccagg
     6061 ggcataaagt cgcattgctg gacattccgg ctaatggtga aattattcgt tatggcgaag
     6121 tgattggtta cgccgtgcgt gcaatcccac gcggaagctg gatcgacgaa tcaatggttg
     6181 tactaccgga agcgccgccg ttacacacgc tgccactggc aaccaaagtc ccggaaccct
     6241 taccgccgct ggaaggatac acctttgagg gctatcgcaa tgccgatggc agcgtgggca
     6301 ccaaaaacct gctcggtatc accaccagcg tccactgtgt ggcaggcgtg gtggactatg
     6361 tagtaaaaat cattgaacgc gatctgctac cgaaataccc gaacgtcgat ggcgtggtgg
     6421 ggctgaatca tttgtacggt tgtggcgtgg cgattaacgc accggcggca gttgtaccta
     6481 tccgtaccat tcacaatatt tcgctgaatc ctaactttgg cggcgaagta atggtgattg
     6541 gcctgggttg tgaaaagttg cagcctgagc gcctgctgac tggaacggat gatgtgcaag
     6601 ctattccagt agaaagcgcc agcattgtca gtttgcagga tgaaaagcat gtcggttttc
     6661 agtccatggt cgaggatatt ttgcagatcg ccgaacgcca tctacaaaaa ctgaatcaac
     6721 ggcagcgaga aacctgcccg gcttcagaac tggtcgttgg tatgcagtgc ggtggcagcg
     6781 atgcgttttc tggtgtaacg gcaaacccgg cggttggcta tgcgtctgat ctactggtgc
     6841 gctgcggcgc aacggtgatg ttttcagaag taacggaagt gcgtgacgcg atccatctgc
     6901 tgacaccacg cgcagtgaac gaagaggtcg gcaaacggct gctggaggag atggagtggt
     6961 acgataacta tctcaatatg ggaaaaaccg accgcagcgc caacccttcg ccgggcaaca
     7021 agaaaggcgg tctggcaaac gtggtagaga aggcactcgg ctccattgct aaatcgggta
     7081 aaagcgcaat tgttgaagtg ctgtcgcccg gtcaacgccc gactaaacgc ggattaattt
     7141 acgccgcgac gccagccagc gattttgtct gtggcacgca acaggtggct tcgggtatca
     7201 cagtgcaagt gtttacgacc ggtcgtggta cgccgtacgg cctgatggcg gtacccgtca
     7261 ttaaaatggc aacccgcacc gagctggcga accgctggtt tgatttaatg gatattaatg
     7321 cgggcaccat cgctaccggc gaagaaacta ttgaagaggt gggctggaag ttgttccact
     7381 ttattctcga cgtcgccagc gggaagaaga aaaccttctc ggatcaatgg gggctgcata
     7441 accagctggc ggtgtttaac ccggcaccgg tgacctgatt tcttttggaa cgtccctcgc
     7501 aaaacatggc cttagtgcca tgtttttatt gtttaaagcc cccacgtcca ttaataatgc
     7561 atttgcatta ccttagttca agcttataat ttgagcagaa aacaggctgt aaaaggacag
     7621 tgaatcatgc ccgctaatgc tcgctctcac gctgtactga ccactgaatc aaaggtcacg
     7681 atacgcggac aaacaactat ccccgcgcca gtgcgtgagg ccttaaaact gaagccaggc
     7741 caggacagca ttcattacga aattctgcct ggtgggcaag tatttatgtg ccgactggga
     7801 gatgaacagg aggatcatac tatgaatgca tttttgcgtt ttctggatgc agatatccag
     7861 aacaacccgc aaaaaactcg tccattcaac attcaacaag gaaagaaact tgtcgctggc
     7921 atggacgtca acattgatga tgagattggc gacgacgaat aatggatttt ccacaaaggg
     7981 ttaatggttg ggcgctatat gctcatccct gttttcagga aacctacgac gctttagttg
     8041 ccgaagtcga gacattaaag ggaaaagatc ctgaaaatta tcagagaaaa gccgccacaa
     8101 agttattggc ggtagtccat aaagtgattg aggagcatat cacggtcaat ccatcatcac
     8161 cggcattccg tcatggcaag tcgttaggct ctgggaaaaa taaagactgg tcacgggtaa
     8221 aatttggtgc tggtcgttat cgtctcttct ttcgttatag tgaaaaagag aaagtcatca
     8281 ttctgggatg gatgaacgat gaaaacactc tgcgcaccta cggtaaaaaa acagatgcct
     8341 ataccgtatt cagcaaaatg ttaaaaagag gacatcctcc tgccgactgg gaaaccctca
     8401 cccgagaaac agaagaaacc cattgatggt gttcacaatg cccgccgaac gcattcgtgt
     8461 tgaccagcgg gcatcctttc tcactccccg accagaatca cttcaacccc agcctttcgc
     8521 agtccttcca ggctatccgc aggaatgcct tcatcaacaa tgatcatgtc gatacgttga
     8581 gtatcaatga tcttatgtaa actggaacga ttgaacttac tggaatcggt gaccacgatg
     8641 atccgttccg caacttcgca catccgacgg tttaaacgag cttcatcttc attatgtgtg
     8701 ctgacgccgc gctccagatc gatcgcatct acaccaagaa acagcatatc gaagtggtaa
     8761 ttttgcagcg attgctcagc ctgatcgccg taaaaagatt gcgactgacg gcgcaaatgc
     8821 ccgccggtca tcagcagctc aacgccttcc gcttccagca acgcattagc cacgttcata
     8881 ccgttggtca tcgcaattac gtcagtgtgc ttgcgcatca gacgagcaat ctcaaaagtg
     8941 gtggtcccgg aatcgaggat aacccgatga cctggctgaa tcaactcaac ggcagctttc
     9001 gcaacgctgc gtttcatcgc ggtgttcagt gcgcttttat cttccactga tggctcgact
     9061 gacggcgtcg tgctatcgca gatcaacgcg ccaccatagg cacgcacagc gatcccctgc
     9121 ttttccagaa acgccagatc gttgcggatc gtcacagtag atacgccata caatgccgac
     9181 agatcgttaa cctgcacact cccttgctgt cgcagacgct gaatgatctg ttctcgtcgc
     9241 tcgctggtgc cagtcactcg cttctcacct gaagcgtcgg tattactcat agtaagtcct
     9301 ttcgtaaaac tttcgtttca tttcgttttg cctattaacg cctttctatt aagcaaatgc
     9361 aagcccacct tgcccattga cgcaagctac tctcgtttca ttgactttca ttatgtttct
     9421 tttgtgaatc agatcagaaa actattatct ttcgttttat ttttatctca ccatgacgca
     9481 gtatcaactg aaacaaaacg aaagattaat atcgcagtaa tctgaactgg agaggaaagt
     9541 gaaacatctg acagaaatgg tgagacagca caaagcgggc aaaacaaatg gaatttatgc
     9601 cgtttgttcc gcacatccgc tggtgctgga ggctgcaatc cgctacgcca gtgcaaacca
     9661 aacgccgtta ctgattgaag caacctccaa tcaggtagac cagttcggcg gttataccgg
     9721 aatgacgccc gccgattttc gcggctttgt ttgtcagctc gccgactcgt tgaatttccc
     9781 gcaggatgcg ttgattctgg gtggtgacca tctggggcca aaccgctggc aaaacctgcc
     9841 ggccgctcag gcaatggcca atgccgatga tttgattaaa agctacgttg cggcaggatt
     9901 caaaaaaatc caccttgatt gcagcatgtc ctgtcaggac gatccgattc ccttaactga
     9961 tgacatcgtg gctgaacgcg ccgcccgtct ggcgaaagtg gcggaagaaa cctgtcttga
    10021 acactttggc gaagccgatc tggagtatgt cattggtacc gaagtgccgg tacctggcgg
    10081 cgcgcatgaa accttaagcg agctggcggt caccacgccg gatgccgccc gcgccacgct
    10141 ggaagcccat cgtcacgcct ttgaaaagca aggtttgaat gccatctggc cacgcatcat
    10201 tgccctggtg gttcaacccg gcgtcgaatt cgatcacacc aacgttattg attatcagcc
    10261 cgccaaagcg agcgccttaa gccagatggt cgaaaactac gaaacgctga ttttcgaagc
    10321 gcactctacc gattatcaaa cgccgcaatc gctgcgccag ctggtgattg accactttgc
    10381 cattctgaaa gttggcccag cgctgacctt cgccctgcgt gaagctctgt tctctctggc
    10441 ggcgattgaa gaagaactgg tgccagcgaa agcctgttct ggtctgcgtc aggtgctgga
    10501 agacgtgatg ctcgaccgcc cggaatactg gcaaagccac taccacggtg acggcaacgc
    10561 gcgtcgtctg gcgcgtggtt atagctactc ggatcgcgtg cgctattact ggccggacag
    10621 ccagattgat gacgctttcg ctcatctggt acgtaatctg gcggattcac caattccgct
    10681 gccgctgatc agccagtatc tgccgctgca gtacgtgaaa gttcgctccg gcgagctgca
    10741 gccaacgcca cgggaactca ttatcaacca tattcaggac atcctggcgc agtaccacac
    10801 agcctgtgaa ggccaataag caaaacaaag aggaacacgc tatgccaaat attgttttaa
    10861 gccggattga tgaacgcttg attcacggtc aggtcggcgt tcaatgggtc ggatttgcgg
    10921 gggcaaatct ggtgctggta gccaacgatg aggttgccga agatccggta caacaaaacc
    10981 tgatggaaat ggtactggca gaagggatcg ccgtacgttt ctggacgctg caaaaagtta
    11041 tcgacaacat tcatcgcgcc gccgatcgac agaaaatcct gctggtttgt aaaacacccg
    11101 ccgatttcct gacgctggtg aaaggtggcg ttccggtgaa tcgcattaac gttggcaata
    11161 tgcactacgc caatggcaaa caacaaatcg ccaaaacggt ttctgtggat gcgggcgata
    11221 tcgcagcatt taacgacctg aaagccgctg gggtggaatg cttcgttcag ggcgtcccga
    11281 cagagcctgc tgtggacctc tttaaattac tttgagggat tcatcatgga aatcagcctg
    11341 ttgcaggcat ttgcgttggg cattatcgcc tttatcgctg gcctggatat gtttaacggc
    11401 ctaacccata tgcaccgccc ggtggtcctc ggcccgttgg tcgggctggt acttggcgat
    11461 ctgcataccg gaattttaac cggcggtacg ctggaactgg tgtggatggg gctggcccca
    11521 ctggcgggcg cacagccgcc taacgtgatt atcggtacta tcgtcggcac ggcgtttgcc
    11581 attactactg gcgtgaaacc cgatgtcgca gtcggtgtcg ccgtaccttt cgctgtcgca
    11641 gtacagatgg ggattacctt cctgttctcg gtgatgtccg gcgtgatgtc tcgctgcgac
    11701 cggatggcgg agaacgccga tactcgcggc attgaacgcg tgaactatct ggcactgctg
    11761 gcactcggca ctttctattt tctctgcgct ttcctgccta tctacttcgg cgcggaacat
    11821 gcaaaaacca tcattgatgt cctgccgcaa cgattaatcg acggcctcgg cgtcgcgggc
    11881 ggcatcatgc cagcaatcgg ttttgccgtg ctgctgaaaa tcatgatgaa aaacgtctac
    11941 atcccctact tcatcctggg ctttgttgcc gccgcctggc tcaagttacc agtgctggct
    12001 attgctgccg ctgcgctggc gatggcgctg atcgacttgc tgcgtaaatc tcctgaaccg
    12061 actcaacctg cggcacagaa agaggaattt gaagatggca tctaatcaaa ccaccctgcc
    12121 gaacgtcagt gaaaacgaag aaacattgct gactggcgtc aatgaaaacg tgtatgaaga
    12181 tcagagcatt ggcgcggagc tgacgaaaaa agatatcaac cgcgtcgcct ggcgttccat
    12241 gctgttacaa gcgtctttta actacgaacg tatgcaggct tctggctggt tgtacggtct
    12301 gctgcctgct ctgaaaaaga tccacactaa taaacgcgac ctggcgcgcg ccatgaaggg
    12361 gcatatgggt ttcttcaata cccatccgtt tctggtgacg tttgttatcg gcattatcct
    12421 tgcgatggag cgttctaagc aggacgttaa cagtattcag agcaccaaaa ttgccgtcgg
    12481 tgcgccgctc ggcgggattg gtgatgcgat gttctggctg acgctactgc cgatttgtgg
    12541 cgggataggc gcaagccttg cgcgacaagg ctctattctt ggcgcagtcg tctttattgt
    12601 gctgttcaac gtggtacacc tggggctgcg ttttggtctg gcgcattatg cttaccgcat
    12661 gggcgtggcg gcgattccac tcattaaagc gaataccaaa aaagtcggcc atgctgcgtc
    12721 tatcgttggg atgacggtaa tcggcgcgct ggtggcaacc tatgttcgtt taagcaccac
    12781 gctggaaatc acagcgggcg acgcagtggt taagttacag gctgatgtta tcgacaaact
    12841 gatgcctgcc ttcttaccgc tggtctacac cctgaccatg ttctggctgg tacgccgcgg
    12901 ctggagtccg ctgcgcctga ttgcagttac cgtggttctc ggcatcgtcg gtaaattctg
    12961 ccatttcctt taaatacaat gaggtattag atgttaagta ttattttgac agggcatggc
    13021 gggtttgcca gtggcatgga aaaagcaatg aagcaaatcc tcggcgatca atcgcagttt
    13081 attgccatcg attttcccga aacctcaagc accgcgctgc ttacatcgca gcttgaagag
    13141 gccatcgccc aactggattg tgaagacggc attgtttttc tgacggattt gctgggcggc
    13201 acgccgtttc gcgtagcttc aacgctggcg atgcaaaaac cgggctgtga agtgatcacc
    13261 ggcaccaatt tgcaactgct actggagatg gtgctggagc gcgaagggtt aagcggtgaa
    13321 gagttccgtg tgcaggcgct ggagtgtgga catcgtgggc tgaccagtct ggtagacgag
    13381 ttaggccgct gccatgaaga atgtccggtc gaggaaggaa tatgacaccc gttctgcgcg
    13441 ccagaaggct gctgactgaa gagggatggc tcgatgacca tcagttgcgt attgctgacg
    13501 gtgtcatcgc agcaatcgaa ccgattccag tgggcgtgac tgaacgcgat gcggaactgc
    13561 tctgccccgc ttacatcgac acccatgtac atggtggtgc gggcgttgat gtaatggatg
    13621 acgcgccgga tgtactcgac aagctggcaa tgcacaaggc acgcgaaggt gtcggcagtt
    13681 ggttgccgac taccgtaacc gcgccgctta ataccattca tgcggcgctg aaacgtattg
    13741 ctcaacgttg ccaacgcggc ggacctggtg cgcaagtgct ggggagttat ctcgaaggac
    13801 cgtacttcac gccgcagaat aaaggcgcgc atccgccgga gttgtttcgc gagcttgaaa
    13861 ttgccgagct ggatcaattg attgccgttt ctcagcacac cttacgcgtg gtagcgctgg
    13921 caccggaaaa agaaggggca ttgcaggcca tccgccatct taaacagcaa aacgtaaggg
    13981 tgatgctggg gcatagcgcg gcgacctggc aacaaacccg cgccgcgttt gatgctggtg
    14041 ccgacggcct ggtgcattgc tataacggga tgacaggttt acatcaccgc gaaccgggaa
    14101 tggttggcgc gggattaacg gacaagcgcg cctggctgga actgatagcc gatggtcatc
    14161 atgtgcatcc ggcggcgatg tcgctgtgtt gttgctgtgc gaaagagaga atcgtactga
    14221 tcaccgacgc gatgcaggca gctgggatgc cggatggtcg ctatacgtta tgtggcgaag
    14281 aagtgcagat gcacggtggc gttgtccgta ccgcgtccgg tgggctggcg ggcagtacgc
    14341 tatctgtcga tgcagcagtg cgcaacatgg tcgagttaac gggcgtaacg cctgcggaag
    14401 ccatccatat ggcatcgctg catccggcgc gaatgctggg tgttgatggt gttctgggat
    14461 cgcttaaacc gggcaaacgc gccagcgtcg ttgcgctgga tagcgggcta catgtgcaac
    14521 aaatctggat tcagggtcaa ttagcttcgt tttgatggtt tgctccatta ttgggccttc
    14581 acttccctcg taaggccttt ctttttcttt ctttttgatc tgtgcagcgg tgtcggatgc
    14641 gacgctaacg cgtctgatcc gacctacggt acgccgcatc cggcaatcgg tgaacatagc
    14701 gacgtcatta cgcaattctt taatcttccc ttgtcttttc tttcaacgat cacagatttc
    14761 gttttatttc ctttttctcc attgaacttt cggtttcttt tctatagatt ttaatcaacg
    14821 aaagacatca ccaagtgaaa tgaaacgaaa ggcaagtgaa agcgacaacg cccgacgtca
    14881 agttcatcag actaaggatt gagttatgcc agaaaattac acccctgctg ccgccgcaac
    14941 cggtacatgg actgaagaag agatccgcca tcagcctcgc gcatggatcc gttcactcac
    15001 caacatcgac gcgctacgtt ccgcgctcaa taacttcctt gaaccgttac tgcgcaaaga
    15061 gaatctgcgg atcatcctga ccggagccgg aacgtcggca tttatcggtg acatcatcgc
    15121 gccgtggctc gccagccata ccggtaaaaa cttcagcgcc gtaccgacca ccgatctggt
    15181 caccaatccg atggactacc tgaatccagc tcatccgctg ctgttgatct ccttcggtcg
    15241 atccggcaac agcccggaaa gcgtcgccgc cgtggaactg gcaaatcaat ttgtacccga
    15301 gtgctatcac ctgccgatca cctgcaacga agcgggcgct ctttaccaaa acgcgatcaa
    15361 cagcgacaac gcgtttgccc tgctgatgcc cgcagaaacg cacgatcgcg gctttgcgat
    15421 gaccagcagc attaccacca tgatggccag ctgcctcgcg gttttcgcac ctgagacgat
    15481 caacagccaa accttccgcg acgtggcgga tcgttgccag gcgatcctga cctcattggg
    15541 cgatttcagc gaaggtgtgt ttggttacgc accgtggaaa cggatcgttt atctcggcag
    15601 cggtggctta cagggcgcag cacgcgagtc ggcgctgaaa gtgctggaac tgactgcggg
    15661 taaactggcg gccttttatg attccccgac cggattccgt catggcccga aatcactggt
    15721 cgataacgaa acgctggtgg tggtatttgt ctcaagccac ccttacaccc gtcagtatga
    15781 tcttgatctg ctggctgaac tccgccgtga caaccaggca atgcgcgtaa tcgccatcgc
    15841 cgcggaaagc acagacatcg ttgctgccgg tccacatatt atcctgccgc cgtcacgtca
    15901 ctttatcgac gttgagcagg cattttgctt cctgatgtac gcccagacgt ttgcactgat
    15961 gcagtcgctg cacatgggca atacgccgga taccccatca gccagtggca ccgttaaccg
    16021 cgtggtgcaa ggcgtaatca ttcatccgtg gcaggcataa gaggatcgca ttatgagcat
    16081 tatctccacc aaatatctgt tacaggacgc ccaggccaat ggctacgcgg tgcctgcctt
    16141 taacattcat aacgccgaga cgatccaggc gatcctcgaa gtgtgcagtg aaatgcgatc
    16201 gccggtgatc ctcgccggaa cgccggggac ctttaaacac atagcgctgg aagagatcta
    16261 cgccctgtgt agcgcctatt ccacaaccta caacatgcca ctggcgctgc atctcgacca
    16321 ccacgaatcg ctggatgata ttcgccgtaa agtccacgca ggcgtgcgca gtgcgatgat
    16381 cgacggcagc cacttcccgt ttgccgagaa cgtaaagctg gtgaaatcgg ttgtcgactt
    16441 ctgtcactcc caggattgca gcgtggaagc agaactgggc cgcctgggcg gtgttgaaga
    16501 tgacatgagc gttgacgccg aaagtgcatt cctgaccgat ccacaagaag ctaaacgctt
    16561 tgtcgagctg actggcgtcg acagcctggc ggtagcgatt ggtacggcgc acggcttata
    16621 cagcaaaacg ccgaagattg atttccagcg gctggcggaa attcgtgaag tggtggatgt
    16681 tcctctggtg ctgcatggtg ccagcgatgt tccggatgaa tttgtccgtc gtactattga
    16741 acttggcgtc acaaaagtga acgttgccac agaattaaaa atagccttcg ctggcgcggt
    16801 taaagcctgg tttgcggaaa atccgcaggg taatgatcct cgttattata tgcgcgtcgg
    16861 aatggatgcg atgaaagaag ttgtcagaaa taaaattaat gtctgtggtt cagcgaatcg
    16921 aatttcagca taatcattag ttttttatga tttatcccaa tgtacttccc gattaatatc
    16981 ggggagtgcc ttaatggaaa aggagataac taaaccttaa taaatacatc agtacaatat
    17041 cgcaacaata atatatttaa aaaaattata ttattcaact ttatggtgag gattacacaa
    17101 tgagcagtcc aaatattctc ttaacccgta ttgataaccg tctggttcac ggtcaggttg
    17161 gcgtgacctg gacatccacc atcggtgcaa atctgctggt agtcgtggat gatgttgtcg
    17221 ctaacgatga tattcaacag aaattaatgg gtattacagc ggaaacctac ggctttggca
    17281 ttcgtttctt tactatcgaa aaaaccatta acgtcatcgg caaagctgca ccacatcaga
    17341 agatcttcct gatttgccgt acgccacaaa cggtacgtaa attggtagaa ggtggtattg
    17401 acctgaaaga tgtcaacgtc ggcaatatgc atttctcgga agggaaaaag caaatcagca
    17461 gtaaatttta tgtcgatgac caggatctcg cggacttacg ttttattaaa caacgtggcg
    17521 tgaatgtttt cattcaggac gtccctggcg atcaaaaaga acaaatccct gactaaatct
    17581 aaaatcgcct taatattggt ttgaggtaat aaaaatgcat gaaataaccc tacttcaggg
    17641 attatccctg gcggcgttag tttttgttct ggggattgat ttttggctgg aagccttatt
    17701 tttattccgc ccgataatcg tttgtaccct aactggcgct attctcggtg atattcagac
    17761 tggcttaatt accggtggtc tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg
    17821 tgttcagccg cccaacccga ttatggcggg tctgatgacc accgtcattg catggtctac
    17881 gggcgttgat gccaaaacag caattggtct tggcctgccg tttagtttgt taatgcagta
    17941 cgtcattctg ttcttctatt ccgctttctc attatttatg accaaagccg ataaatgcgc
    18001 gaaagaggcg gatacggcag cgttttcccg gcttaactgg acaacgatgc tcatcgtcgc
    18061 ttcagcgtat gcggtgattg ctttcctctg tacttacctg gcacaggggg cgatgcaggc
    18121 gctggtgaaa gcgatgcccg cctggctgac ccacggcttt gaagtggctg gcggtattct
    18181 gcctgccgtt ggttttggct tgctgctgcg cgtaatgttc aaagcgcaat atatccctta
    18241 cctgatcgcc ggtttcctgt ttgtttgcta catccaggtc agcaacctgt tgccggttgc
    18301 cgtactgggc gcaggctttg cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc
    18361 gcaaccgcag cccgttacca gtaaaaatga agaagaggac tacagcaatg ggatctgaaa
    18421 tcagtaaaaa agatatcacc cgtctgggct ttcgttcgtc gctgctgcaa gcgagcttta
    18481 actacgaaag gatgcaggcg ggcggtttta cctgggcgat gttgccgatc ctgaaaaaga
    18541 tttataagga cgacaaaccg ggcttaagcg cggcgatgaa agataacctc gaatttatta
    18601 atacccaccc gaatctggtc ggattcctga tggggttatt aatttcgatg gaagaaaaag
    18661 gagaaaaccg cgacaccatt aaaggcctca aagtggcact gtttggccca atcgccggga
    18721 ttggcgatgc gattttctgg tttactttgt tgccgattat ggcgggaatt tgctcatcat
    18781 ttgccagcca gggaaacctg ctggggccga ttctattttt cgccgtttac ctgcttatct
    18841 ttttcctgcg cgtcggctgg acccacgtcg gttattcagt cggcgtgaag gcgatcgata
    18901 aagtgcgaga gaactcgcag atgattgccc gttcggcaac catcctcggg atcacggtaa
    18961 tcggcgggct gatcgcttcg tatgtgcata ttaacgtggt gacatcgttt gccatcgaca
    19021 atacccacag cgttgcgctg cagcaggatt tcttcgataa agtcttcccg aacattttac
    19081 cgatggccta caccctgctg atgtattact tcctgcgggt gaaaaaagcg catccggtgc
    19141 tgttaatcgg cgtgactttt gtgctctcta ttgtttgttc cgcattcggc attttgtaaa
    19201 tggaacgagg cactgcgtct ggtggtgcct ctttacttaa ggaatttcat cctgtgcaaa
    19261 cccttcagca agttgaaaac tatacggcgt taagtgaacg tgccagcgaa tatttattgg
    19321 ccgtgatccg tagcaaaccg aatgccgtga tttgcctggc gaccggagcc acgccattac
    19381 tgacgtatca ttatctggta gaaaaaatcc accagcagca ggttgatgtc agccagctca
    19441 ccttcgtgaa gctcgacgaa tgggtggatc tgccattaac gatgccaggc acctgcgaaa
    19501 ctttcctgca acagcatatc gtgcagccgc tggggctacg tgaagaccag ctcatcagct
    19561 ttcgctccga agagataaat gagacagagt gcgaacgggt aacgaacctg attgcgcgca
    19621 aaggcggtct ggatttatgc gttctcggat tggggaaaaa cggtcatctt gggctgaacg
    19681 aaccgggaga aagcctgcaa ccggcctgcc atatcagtca acttgatgcc agaacacagc
    19741 aacatgagat gttaaaaacc gcgggtcgcc ccgtgactcg tgggatcacc ttaggcctga
    19801 aggatattct caatgcccgc gaagttttgt tactggtgac tggcgaagga aagcaggatg
    19861 cgacagatcg ttttctcacg gctaaagtct ctaccgctat cccggcttca tttttatggc
    19921 tgcacagtaa ctttatttgt ttaattaaca cctaaccaga tgaaaaattt gctcaaaatg
    19981 tttaaattaa cttatgtaac agtcacgcat tatattaaat aacatttgac tgggttgaac
    20041 ataacgccga tagcaaaagg agttatgttc aacaatttaa ttttagatat acatataact
    20101 tgttctatta ataaatcacc tcgtattctg actcgccccc tggcgcagca aagtcaggca
    20161 tttatactct tttgaataca tagaaaattg atatcaatat aatgaaaata tcaaatattt
    20221 gcatataaat ataatcttaa agttcagtct atttaatgtt caatgaaata tttctgcctg
    20281 tataatcttt aaagatgttg aacatatatt cacattaaat atgattatgt acttgttaca
    20341 aggataaggt tatatatgaa taaagttaca aaaacagcta ttgcgggctt acttgcgctt
    20401 ttcgcaggta atgccgctgc aaccgatggt gaaatagttt ttgatggcga gattttaaaa
    20461 tccgcttgtg aaatcaatga ctctgataag aaaattgaag ttgctcttgg tcactataat
    20521 gccgaacagt ttcgtaatat tggcgagcgt agcccaaaaa ttccattcac tattccttta
    20581 gttaactgcc caatgactgg gtgggagtac gataacggca acgttgaagc gtctttccgt
    20641 ctatggctgg aaacgcgaga taatggcacc gtccctaact tccctaatct ggcaaaagta
    20701 ggctcttttg ctggcatagc ggcaacaggt gtgggtatcc gtattgacga tgcggaaagc
    20761 ggaaacatta tgccactgaa tgctatgggc aatgataaca cggtttatca gatcccggcg
    20821 gaatccaatg gtattgtcaa tgttgacctc atcgcttact acgtatcaac cgtagttcca
    20881 tcagaaatca ccccagggga agcagacgct atcgttaacg taacgctgga ttaccgttaa
    20941 tacgttaggg cgttatctga cctgtcagat aacgcccttt tccttcctct ttctcgttgt
    21001 atcaggttga aaaataacta tgtcaaaacg aacattcgcg gtgatattaa ccttgttgtg
    21061 tagcttctgt attggccagg cgcttgcagg aggaatcgtt ttacagcgaa cgcgagtgat
    21121 ctatgatgcc agccgcaaag aggctgcgtt acctgtcgca aacaaaggcg cagaaacgcc
    21181 ttatttactg caatcatggg tagataatat agatggtaaa agccgtgccc catttattat
    21241 aaccccaccg ctatttcgtc ttgaggctgg cgatgactca tcactgcgaa ttattaaaac
    21301 agctgataac ctgcctgaaa ataaagagtc gctgttctac attaatgttc gtgccattcc
    21361 agcaaagaaa aaatcagatg atgttaatgc taacgagttg acgctggtat ttaaaacacg
    21421 gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg gtaaacgatg cgtggaaatc
    21481 actggaattt aaacgtagtg accattcact caatatatat aacccaactg aatattacgt
    21541 cgtatttgcc ggactggcag tcgataaaac cgatctcaca agcaaaattg aatatatcgc
    21601 gcccggagaa cataaacagt taccacttcc tgcatctggc ggaaagaacg tgaaatgggc
    21661 tgcgatcaat gattatggcg gcagttccgg gacagaaact cgtccactgc aataaaaaat
    21721 ataaaaacac aggtcatcag ggaatgccac aacgacacca ccagggacat aaacgcacac
    21781 cgaaacagtt ggcgctcatc atcaaacgtt gtttgccgat ggtgctcact ggcagcggca
    21841 tgctttgcac taccgctaac gccgaagagt attatttcga ccccattatg ctggaaacca
    21901 caaaaagtgg tatgcaaaca accgatctgt cgcgtttttc aaaaaaatat gcacaactac
    21961 caggaactta tcaggttgat atctggctga ataaaaagaa ggtttcacag aaaaaaatta
    22021 catttaccgc caatgcagag caacttctgc agccacagtt tacggtagaa caactacgtg
    22081 agctgggtat taaagtggat gaaatcccgg cgctggctga aaaagatgac gatagcgtga
    22141 tcaactcgct tgaacaaatc attcccggta cagctgctga atttgatttc aatcatcagc
    22201 aacttaattt gagcattccc caaattgcac tgtaccgtga tgcaagaggt tacgtctccc
    22261 cttctcgttg ggacgatggt ataccaacgc tgtttaccaa ctactcgttt acaggttctg
    22321 ataaccgtta ccgccagggc aatcgtagcc aacgacagta cctgaatatg caaaatggtg
    22381 ctaattttgg cccctggcga ttacgcaact attccacatg gacacgcaac gatcagacat
    22441 caagctggaa taccatcagt agttatttac aacgtgatat caaggcattg aagtctcagt
    22501 tgcttctggg agaaagcgcc accagcggca gtattttttc cagctacacc tttactggcg
    22561 tgcaactcgc ttccgacgat aatatgctgc caaacagcca gcgcggattt gccccaacgg
    22621 tacgcggtat cgcaaacagt agtgcaatcg tgactatcag gcaaaatggt tatgtgatct
    22681 atcaaagcaa cgtgccagcg ggtgcctttg aaattaacga tctctacccc tcttccaaca
    22741 gcggcgattt agaagtcact attgaagaaa gtgacggtac acaacgtcgc tttatccagc
    22801 cttattcttc attacccatg atgcagcgac ctgggcatct aaagtatagc gcgaccgctg
    22861 gacgctatcg cgctgatgca aacagtgata gcaaggaacc cgaatttgct gaagccacgg
    22921 caatatatgg tttgaataat acttttacgc tgtatggcgg cctgctcggt tctgaagatt
    22981 attatgcgct gggaatcggt atcggcggca cacttggcgc actgggcgcg ttgtcgatgg
    23041 atatcaacag agctgacacc caattcgata accagcactc ttttcatggc tatcaatggc
    23101 gtacgcagta catcaaagat atcccggaaa ccaacaccaa tatcgctgtc agctactatc
    23161 gctataccaa cgatggctat tttagtttta atgaagccaa tacccgcaat tgggactata
    23221 acagtcgcca aaaaagtgaa attcaattca acatcagcca gacaatattt gatggggtaa
    23281 gtctgtatgc ctccggttcg cagcaagact attggggcaa taacgataaa aacaggaata
    23341 tctctgttgg ggtttccggc cagcaatggg gagttggtta cagcctgaat tatcaataca
    23401 gccgctacac tgatcaaaat aatgaccgcg cactctcttt gaatctcagt attccgttag
    23461 aacgctggtt accgcgtagc cgggtttcct atcagatgac cagccagaaa gatcgcccaa
    23521 cccaacatga aatgcgtctt gatggctcac tgctggatga tggtcgcctg agctatagcc
    23581 tggaacaaag tctggatgac gataacaacc ataacagtag cctgaacgcc agttaccgtt
    23641 caccttatgg caccttcagt gccggataca gctacggtaa tgacagcagc caatacaatt
    23701 acggcgttac cggcggcgtg gttatccatc ctcatggcgt gacgctctcg caatatctgg
    23761 gcaacgcttt tgcgcttatc gatgctaacg gagcatctgg cgtgaggata caaaactatc
    23821 cggggattgc taccgatcct tttggctatg cagtggttcc ttatctcaca acttatcagg
    23881 aaaaccgtct ctcggtagat actacgcagc tgcccgataa cgtcgatctt gaacaaacaa
    23941 cacagtttgt ggtgcccaac agaggtgcaa tggtagcggc gcgtttcaac gccaatatcg
    24001 gttatcgcgt acttgttaca gtcagcgatc gcaacggtaa accgttgccc tttggcgctc
    24061 ttgccagcaa cgatgatacg gggcaacaaa gtatcgtcga tgagggcggc atactatatc
    24121 tctctgggat atcgagtaaa tcacaaagct ggactgtacg ctggggaaat caggcagatc
    24181 aacaatgtca gtttgctttt agtacaccgg attcagaacc cacaacctct gtattacaag
    24241 gcacggcgca gtgccattaa ggataaaaaa atgaaaagag cgcctcttat aacaggactt
    24301 ttgttgatat ccacatcctg cgcttatgcc tcctcaggag ggtgtggagc cgatagcact
    24361 agcggtgcga caaattacag cagtgtggtt gatgatgtta cggtgaacca gacagataac
    24421 gtgacaggac gggagtttac ctctgcaacg ctaagtagca ctaactggca atacgcctgt
    24481 tcctgctctg cgggtaaggc agttaaactt gtctatatgg tcagccccgt acttaccacc
    24541 actggacatc agacaggata ttacaaactc aatgacagcc tggatattaa aaccacatta
    24601 caggcaaacg acattccagg actcacaacc gaccaggttg tctctgttaa cacccgattc
    24661 acacagataa aaaacaacac ggtatattct gctgcaaccc aaacgggtgt ttgccagggt
    24721 gatacgtctc gttatggacc cgttaatatt ggtgcgaaca ccacctttac cctgtatgtc
    24781 accaagccat ttctcggctc gatgaccatt ccgaaaacgg atattgccgt cattaaaggc
    24841 gcgtgggtcg atggaatggg aagcccgtct acaggtgact tccatgattt agtcaagtta
    24901 tcgattcagg gaaatctcac cgccccacag tcgtgcaaaa ttaatcaggg cgatgttatt
    24961 aaggttaatt ttggattcat caatggtcag aagtttacca cccgcaatgc catgccagac
    25021 ggttttactc cagtagactt tgatatcact tatgactgtg gtgatacttc aaagattaaa
    25081 aactcgttgc aaatgcgcat cgacggtaca actggggtag tagaccagta caacctggtc
    25141 gccaggcgaa gaagttcaga caatgtgccc gatgtcggta ttcgtattga aaatctcggc
    25201 ggcggagttg caaatattcc ttttcagaac ggtatccttc ccgttgatcc ttccgggcat
    25261 ggcaccgtca atatgcgcgc ctggccagtt aatctggtcg gtggtgagct ggaaacagga
    25321 aaatttcagg gcacagccac cattaccgtc atcgtgcggt aaaagaagac gttgcgtttt
    25381 gattaacgca acgcttcggc actgttaccc ctgctgctcc agcgcatact tatacagcgc
    25441 atttttcttc acgccgtgaa tttctgcggc cagcgccgcc gcttttttca gcggcagttc
    25501 tgcctgtagc agcgccagcg tgcgcagggc atcggcgggt aagtcttctt cctgtgcttt
    25561 atgaccttcg acaatcagca ccatttcgcc tttgcgacgg ttttcatctt cctttaccca
    25621 cgccagcagc tcgccaacgg gcgcgccgtg aatggtttcc caggttttgg tcagctcacg
    25681 cgccagaacc acgtagcggg attcgcctaa taccgcaacg atatcttcca ggctatctaa
    25741 cagacggtgg gtagattcat aaaaaatcag cgtgcgcggc tccgcttcaa tggcttttag
    25801 cgcatcacgg cggccttttg atttggcagg taaaaagcct tcgtaacaga aacggtcaga
    25861 gggtaaaccc gctgcgctta acgcagtgat agcagcacac ggcccgggta gcggcaccac
    25921 gcggatcccc gattcacggc aggtacgcac cagatggtag ccaggatcgt taattagcgg
    25981 cgttccggca tcggaaacca gcgcaatgtt ttgcccctct tgcagcttcg ccagcagcgt
    26041 ttcagctttt tgttgttcgt tatggtcgtg cagcgcaaac aaccgggcat taatcccaaa
    26101 atgttgcagc aataaaccgg tgtgacgagt atcctcggcg gcaatcagat caacggcctg
    26161 taatacctct aacgcacgct gggtgatatc cgccagattg ccgattggcg tcggtacaat
    26221 gtaaagctgg ccctgagaat tatccgccga ttggtgttgt ttcattgtgt cgtccgtatt
    26281 gccgatttaa tattgagcat tgcgtaaaaa aaatatcact ggatacatta tggtaccctc
    26341 aacattttct cgtttgaaag ccgcgcgttg tctgcctgtt gttctggcag ccctgatttt
    26401 cgccggttgt ggcacccata ctcccgatca gtccactgct tatatgcagg gtacggcgca
    26461 ggctgattct gccttttatc ttcagcagat gcagcaaagc tctgatgata ccaggatcaa
    26521 ctggcaatta ctcgccattc gtgcactggt gaaagaaggt aaaaccgggc aggcggtgga
    26581 gttgtttaac caactaccgc aagaactgaa cgatgctcag cgccgcgaga aaacactgct
    26641 ggcggtagag attaaactgg cgcagaaaga ttttgctggc gcgcaaaact tgctggcgaa
    26701 aatcacacct gccgatttag aacaaaacca gcaagcccgt tactggcagg caaaaatcga
    26761 tgccagccag gggcgtcctt ccattgattt actgcgcgcg ttaattgctc aggaaccgct
    26821 gctcggcgcg aaagaaaagc agcagaatat tgacgccacc tggcaggcgc tctcctccat
    26881 gactcaggaa caggcgaata cgctggtgat caacgccgac gaaaatattc tgcaaggctg
    26941 gctggatctg cagcgcgtct ggtttgataa ccgtaacgat cccgacatga tgaaagccgg
    27001 gatcgccgac tggcagaaac gttatccgaa caacccgggc gcgaaaatgc tgccaacgca
    27061 gttggttaac gtaaaagcgt ttaaaccagc ctcgaccaac aaaatcgccc tgctgttgcc
    27121 actgaatggc caggcagcgg tatttggtcg cactattcag caaggctttg aagcggcgaa
    27181 aaatatcggc actcagccag tggcagctca ggtagctgcc gcacctgccg cagacgtagc
    27241 tgaacaacct cagccgcaaa ccgtggatgg cgttgccagc ccggcacaag cctcggttag
    27301 cgatctgacc ggtgaacagc ctgcagccca gccggtgcct gtaagcgccc cggcgacaag
    27361 caccgcagcg gtaagcgcac ccgcaaatcc atccgcagag ctgaaaatct acgatacctc
    27421 atcacaacca cttagccaga tcttaagcca ggttcagcag gatggcgcga gtattgtggt
    27481 cggtccgttg ctgaaaaata acgttgaaga gttgctgaag agcaacactc cgctgaacgt
    27541 actggcactg aaccagccgg agaatatcga aaatcgcgtc aatatttgtt acttcgcgct
    27601 ttcaccggaa gacgaagcgc gcgatgcagc gcgtcatatt cgtgaccagg gtaaacaagc
    27661 gccgctggtg ctgatcccac gcagttcatt gggcgatcgc gtagccaatg cgtttgcgca
    27721 agagtggcag aaactgggcg gcggcaccgt tctgcaacaa aaatttggtt ccaccagcga
    27781 attacgcgcg ggtgttaacg gcggttctgg tattgcttta acgggtagcc cgattactct
    27841 cagagcgaca accgactccg gcatgacgac caacaatcca acgctgcaaa ccacgccaac
    27901 cgatgaccag ttcaccaata atggcggtcg tgtcgatgcg gtgtacattg tggcaacgcc
    27961 gggtgaaatc gcttttatca aaccgatgat cgccatgcgt aacggtagcc agagcggtgc
    28021 aacgctgtac gccagctccc gcagtgcgca agggaccgct ggcccggatt tccgactgga
    28081 gatggaaggc ttgcagtaca gcgaaatccc gatgctggca ggcggtaatc taccgttaat
    28141 gcagcaggca ctcagcgcgg tgaataacga ttattcactg gctcgcatgt atgcgatggg
    28201 cgtcgatgcc tggtcgctgg caaatcattt ctcacaaatg cgccaggttc agggttttga
    28261 aatcaacggt aataccggaa gcctgacggc taacccggat tgcgtgatta acaggaagtt
    28321 atcatggcta cagtaccaac aaggtcaggt agtccccgtc agttaaccac caaacagacc
    28381 ggcgatgcgt gggaagcaca agcgcgtcgc tggctggaag gcaaaggact gcggtttatc
    28441 gccgctaacg tgaacgagcg tggcggcgag atcgatctga taatgcgtga aggccggacc
    28501 accatttttg tcgaggtacg ctatcgccgc tctgcgcttt atggcggcgc ggcagccagt
    28561 gtgacccgca gcaaacaaca caaattatta cagactgccc gcttgtggct cgcgcgtcat
    28621 aatgggagtt ttgatactgt ggattgccgg ttcgatgtgg tagccttcac cgggaatgag
    28681 gttgagtgga ttaaggatgc ctttaatgac cactcataat taaggtttaa ggattagcgt
    28741 gcaagaaaga attaaagctt gcttcactga aagcattcaa actcaaattg cggcggcaga
    28801 ggcgcttccg gatgccatct cccgtgcagc catgacgctg gttcagtctc tgctcaatgg
    28861 caacaaaatc ctctgttgtg gtaatggaac ttccgctgcc aatgcacagc attttgctgc
    28921 cagcatgatc aaccgtttcg aaacggagcg gcccagctta cctgccattg cactaaatac
    28981 tgataatgtt gtcttaacgg cgattgccaa cgatcgctta catgatgaag tgtatgcaaa
    29041 acaggtgcgg gcgctgggtc atgcgggaga tgtattgtta gccatttcca cccgtggcaa
    29101 cagccgcgat attgtaaaag cggtagaagc tgccgttacg cgtgatatga ccattgtggc
    29161 attgaccggc tatgacggcg gcgaacttgc aggtttgtta gggccacagg atgtggagat
    29221 ccgcattcct tcgcatcgta gtgctcgcat tcaggaaatg catatgctga cggtaaattg
    29281 cctgtgcgat ctgatcgata acacgctttt ccctcaccag gatgattaag gagaatacat
    29341 gaaggcatta tcgccaatcg cagtccttat ttccgcgctg ctgttgcaag gttgtgttgc
    29401 cgctgccgta gtgggtaccg ctgctgtggg taccaaagcc gcaactgacc cacgcagtgt
    29461 cggcacccag gtggacgatg gtaccctgga agtgcgcgtg aacagcgcat tgtcgaaaga
    29521 cgaacagatt aagaaagaag cgcgcattaa tgtaacggcc tatcagggca aagtgctgct
    29581 ggttgggcag tcaccaaatg ctgaactttc ggctcgcgcc aaacagattg ctatgggcgt
    29641 agacggtgcc aacgaagtgt ataacgagat tcgtcagggc cagccgattg gtctgggcga
    29701 agcatctaac gatacgtgga tcaccaccaa agtgcgttcg cagctcttaa ccagcgacct
    29761 ggtgaaatcg tccaacgtga aagtgaccac cgaaaacggt gaagtgttcc tgatggggct
    29821 ggtgactgaa cgtgaagcga aagcggcggc agatattgcc agccgggtga gcggcgtgaa
    29881 gcgggtaact acggcgttta cgtttattaa atagcagccc cttgtaatgc ctgatgcgac
    29941 gcttgccgcg tcttatcagg cctacaacga tacaaaccgt aggtcagata aggcgtttac
    30001 gccgcatccg acatgttttc cctcaaatca gaacaacagc gccagcccgc cgacaatcac
    30061 accagacact gccaccatcg cccctgtcag ccataaggct ttcgccggga acgctttgcg
    30121 cagcataatc agtgacggca aactcactgc cgggagcgtc atcaacaatg ccagcgccgg
    30181 agcggttccc atacctgcca gcatcatcgt ttgtacaatc ggaatttctg ctgccgtggg
    30241 aatgacaaac aagcatcctg ctaccgccat cgccaccacc cacatcaggc tgttatcgac
    30301 agcaccatcg gcatggggga ataaccagac gcgagcggca cccaacacca gtactgcaag
    30361 gatgtaaacc gggatcgtac tccagaaaag cgtccatagc gccctgcccc agcggctaaa
    30421 aaacccgccc tgtgcttccg gtatgtcaat ttcgaccggt gcctgcgttt gcggtgtttc
    30481 acgcacccat ttttgcacca gcgtcgcaat cagcaacacc atcaccagcc cggccaccag
    30541 acgaatcgcc gcaaaacccc agccgaggac aaagcccata aacaccagcg tcgccgggtt
    30601 taacaccgga ttgcccatcc agaatgccag cgcaccgccc atcgacacct gttggcgacg
    30661 cattcccgcc gcgaccggag ccgcacagca ggtacacatc atgcccggca acgaaaacag
    30721 cgttcccagc agcgtgccgc gaaagcgcga ttgcccaagc gtacgcaaca accagtcacg
    30781 cgggatcagc acctgaatca acgaaccgag gatcaccccc agcaccgccg ctttccatac
    30841 cgcgaggaaa tagatcatcg cgtaatccaa cgccgcctgc catgggttag catccgcctg
    30901 cgcaaggata gatttaccga tactgtgggt ttcggcagca gtaaacgctt tgccgtagta
    30961 aggttcccat ttcacatacc agagaccggc aatgacaacg agaaagaaaa gcgcgggttt
    31021 ccaccactga atgggcgttg ccgcctgaga tgaagactga ccagtcatag cattccccag
    31081 gaataagtta tgataattga gcgcgtgaat attacgctca ctatcaattc ttgggaataa
    31141 ttattcagct cttttgcgta attctgaaga gcttaaaatc gtcacgcctt catgctcagg
    31201 ccgcatcgac tctgccagca tgacccgcgc cacgtctctc gcatcaatgg atttccagtt
    31261 acctggtaac aaacggaaca gcggcgcaaa aagcgtttcg ttcatccgct gtttgctacg
    31321 atcgcccagt aacatcgacg ggcgagcaat ggtcaatttc ggccagttct gggcgattaa
    31381 tgcttcttcc atctcccctt tgacgcggtt atagaaaaac ggcgagtggg cattggcacc
    31441 catcgcactg actaccaaca tatgctgcgc gcccagtcgc cgcccggtta atgcggtatc
    31501 cactaccagc gtgtaatcgg catgaataaa cgcctctttg ctccccgctt ctcgccgcgt
    31561 ggtgccgaga caacaaaaca caatgtcgat gggatcggtg acctgcgcca acgcgtcact
    31621 cagttgcgga tcatggggat taaacacccc gggcatatcg cccaacggac gtcgcgtcgg
    31681 cgcagcaatg gcgttaactt tcggttcgtt aatcaacatc cgcagcaggt gaccgcccac
    31741 cagccccgtt gcgcctgtaa tcagtacctg actcatcttc gctcctttac agaattgtct
    31801 gccttgcgct ccacggctca tgcaccaggc ttaatagacc atgaggtaat tatccccgat
    31861 tgtgggaaat tcgcctcatc caatgcaaca acgcggagga agcatgagta agaaaattgc
    31921 cgttttaatc actgatgaat ttgaggattc agaatttact tcacccgcag acgagttccg
    31981 taaagccgga cacgaagtga ttaccattga aaaacaagcg ggtaaaacgg tgaaaggcaa
    32041 aaaaggagaa gccagcgtga ccatcgataa atccatcgat gaagtgacgc ctgcggagtt
    32101 tgatgccctg ctgctaccgg gcggccattc accggattat ctgcgtgggg acaaccgttt
    32161 tgtcaccttt acccgtgatt ttgtgaatag tggcaaaccg gtgtttgcca tctgtcacgg
    32221 cccgcagttg ctgatcagcg ccgatgtgat tcgggggcgc aaactgaccg cagttaaacc
    32281 gatcattatt gatgttaaaa atgcgggcgc ggaattttac gatcaggaag tcgtggttga
    32341 taaagatcag ctggttacca gccggacacc ggacgatctg ccagcgttta accgcgaggc
    32401 gttacgcctg ctcggtgcct gagtcgcgta gccaaatcat ttttttacca aagcccagcg
    32461 tgttgtcggt gaatttgatt tcatcgagcc ggatttccca caccggggcc gacagcattc
    32521 tggcaaccgg aaagcgacga ttgtacgcct tgcgcgcgag gtcgctttcc tcaccttcca
    32581 gcctgcggat ctcaccttta aactgcacac cgcgaattaa cgctaccgtt ttcggctgac
    32641 cgtttaccgt tccggcaacc gcagcctgcg gcccactcat ctgcgcgtgg cgcgtttttt
    32701 cttccgtcaa aatgtagaag gcgactttct gcgcatcaaa aagataaaag gcattagcgc
    32761 accagagttc cccttcctgc tgcacacacc aagtgacaac atgttgtttt gccagccaac
    32821 ggctgatggc gatgagtgtt tccattgctg ttctctttta tactgtgggc ctgaccttaa
    32881 catgcgacgc agacgatgac accctggttt ctttacttga tccgtaccgc cgacaataag
    32941 ctttataccg ggatcaccac ggatgtcgaa cgccgctatc agcagcacca aagcggcaaa
    33001 ggggcgaaag cactgcgcgg gaaaggagaa ctaacgctgg tgttttccgc gccagtgggc
    33061 gatcgttcgc tggcgttacg ggcggaatat cgcgttaagc aactgaccaa acggcaaaaa
    33121 gagcgtctgg tagcggaagg cgcagggttt gcagagctgt taagcagtct gcaaaccccg
    33181 gagattaaaa gcgattgaaa tgctcgtgat actcaaccag gccggtaacg ccattcagcg
    33241 cgtcatccgc taaacgatgt acctggaagg cgctttcagt gcccggccag cggcaacgca
    33301 gatcgtgatg cgccgccagt tcaaagccga aacgactgta cagcgccgga tcgcccagcg
    33361 tcaccactgc ggcatagccg aactcattaa gcgaatcgag tccttcatag accagttggc
    33421 gtgccagccc ctgtccacgg tatttttcat caaccgccaa cggtgccatg ccgacccatt
    33481 gcaggtcttc gccctgcaca tcaaccggac taaatgccac atagccaatg acctgacctt
    33541 cgtcatctgt cgccaccagc cccagcgtca gaaagccatc ttcacgcaga tcgtgaacca
    33601 gcttcgcttc cgcatcactt tcgaatgagc gacgcagcag ggcatcaata cccggcgcat
    33661 caatgggaat ttctactcga attagcatgg ttcacctacc gatgtctgtt tggtttcagg
    33721 cgcggttttc attcccgcct caacaaaatc cgccagttgc agcagcatca tgcgtaaggc
    33781 tttcggcatt tgctccagtt caatggcgtc catcaggttt ttcacataca gccccagctc
    33841 cgtatcgcct tcaatcacca gccgacgctg gaagaagagc gtatccggat cttgtttacg
    33901 cgccgcaatc atcagcagat cgctggcgtc ggcactaaaa ctcacatcag cttgcgcgtt
    33961 ctggctaacg accagtttgc cattcaccac cgaggtaaac cattgcaggt caatatcacg
    34021 cacatgaata cttaaccagc ggccttcaag aaactccagc tcgccatcat ccagcgcctg
    34081 gcggaattgc cagcttaaga cctgctcaag aacctggcgt tttagcgcaa atggcgtcag
    34141 ttttaccggt acactcaaca gagatggccc caaatgcaca atacgggaac gcagtttatc
    34201 caacacgagt tttactccct gtttcaacaa tcatcctatt ttgccatatc agaaaaataa
    34261 catagcggta taaatcaaca attccatatg aaattgctgc taccaccaat acaactttaa
    34321 ctgccttaaa tcaaaaattg tcgcagcaag gttaactaaa atcccagttc gttaacattt
    34381 ttgcgttttg atagcgcaac cttcaggaaa aattatggag ctgctctgcc ctgccggaaa
    34441 tctcccggcg cttaaggcgg ccatcgaaaa cggcgcagat gctgtttata tcgggctaaa
    34501 agatgatacc aatgcccgtc acttcgccgg ccttaacttt accgagaaaa aattgcagga
    34561 agcggtgagt tttgtccatc aacatcgccg caaacttcac atcgcgatta acacttttgc
    34621 gcatccggac ggttacgccc gttggcagcg cgccgtggat atggcggcgc agctgggtgc
    34681 cgacgcgctg atcctcgccg acctcgccat gctggagtac gccgccgagc gttatccgca
    34741 tattgagcgc cacgtatcgg tgcaggcttc ggcgaccaat gaagaggcga ttaactttta
    34801 tcatcgccat tttgacgttg ctcgcgtggt gctgccgcgc gtgttgtcga ttcatcaggt
    34861 gaaacaactg gcacgggtca cacctgtacc actggaagtc tttgctttcg gcagcctgtg
    34921 cattatgtcg gaaggtcgtt gctatctgtc gtcgtatctg acgggtgagt cgcccaacac
    34981 cataggcgcg tgttctccgg cccgtttcgt gcgctggcaa caaacgccgc aggggctgga
    35041 atcccgcctg aacgaagtgc tgatcgaccg ttatcaggac ggcgaaaacg caggttatcc
    35101 gacgctatgt aaagggcgtt atctggtgga cggcgagcgc tatcacgcgc tggaagaacc
    35161 aaccagtctc aataccctgg aactgctgcc ggagttaatg gcggcgaata ttgcttcggt
    35221 gaaaattgaa ggccgccagc gtagcccggc gtatgtcagc caggtggcga aagtctggcg
    35281 tcaggctatc gaccgttgta aggccgatcc gcaaaacttt gtaccgcaaa gcgcgtggat
    35341 ggagacgctc gggtcgatgt ccgaaggcac gcaaaccact cttggcgcat atcaccgtaa
    35401 atggcagtga gaaaagcaat gaaatattcc ttagggccag tgctgtggta ctggccaaaa
    35461 gagacgctgg aagaatttta tcagcaggcc gccaccagca gcgccgacgt gatttatctt
    35521 ggtgaagcgg tatgcagcaa gcgtcgggca accaaagttg gcgactggct ggagatggca
    35581 aaatcgctcg ccgggagtgg taagcagatt gtgctctcca cgctggcgct ggtgcaggca
    35641 tcatctgaac tgggcgaact gaaacgctat gttgagaacg gtgagtttct gattgaagcc
    35701 agcgatctcg gcgtggtgaa tatgtgcgct gaacgcaaac tgccgttcgt cgccgggcac
    35761 gcgctgaact gctacaacgc ggtgacactg aaaatattgc tcaaacaggg catgatgcgc
    35821 tggtgtatgc cagtggagct ttcccgcgac tggctggtga atctgcttaa tcagtgcgat
    35881 gagctgggca ttcgtaacca gtttgaagtg gaagtcctga gctacggtca tctgccgctg
    35941 gcctactccg cccgctgctt taccgcgcgt tcggaagacc gcccgaaaga tgagtgtgaa
    36001 acctgctgca ttaagtatcc gaacgggcgc aacgtgctgt cgcaggaaaa ccaacaagtg
    36061 tttgtactca atggcattca gaccatgagc ggctacgttt acaacctcgg taacgagctg
    36121 gcatccatgc agggcctggt tgatgtggtc cgcctgtcac cgcagggtac tgacactttc
    36181 gcgatgctcg acgccttccg cgctaatgaa aatggcgctg caccactgcc gttgacggcg
    36241 aacagcgact gtaacggcta ctggcggcgg ctggcaggac tggagctgca agcctaagta
    36301 aatagctcac tttgttaaca actttaacta ctctttaatg cagtattaaa gattaatcgg
    36361 taacaaagtg agctgttatg actgataaaa ccattgcgtt ttcgctactc gatctggccc
    36421 ccattcccga aggttcttca gcgcgagaag cattctccca ctctctcgat ctcgcccgtc
    36481 tggctgaaaa gcgcggctat catcgctact ggctggcaga acaccacaat atgactggca
    36541 ttgccagtgc tgccacgtcg gtattgatcg gctatctggc ggcgaatacc accacgctgc
    36601 atctggggtc tggcggtgtg atgttgccta accactcacc gttggtcatt gcagaacagt
    36661 tcggcacgct taatacactc tatccggggc gaatcgattt ggggctgggt cgtgctccgg
    36721 gtagtgacca acggacaatg atggcgctac gtcgtcatat gagcggcgat attgataatt
    36781 tcccccgcga tgtggcggag ctggtggact ggtttgacgc ccgcgatccc aatccgcatg
    36841 tgcgcccggt accaggctat ggcgagaaaa tccccgtgtg gttgttaggc tccagccttt
    36901 acagcgcgca actggcggcg cagcttggtc tgccgtttgc gtttgcctca cacttcgcgc
    36961 cggatatgct gttccaggcg ctgcatcttt atcgcagcaa cttcaaaccg tcagcacggc
    37021 tggaaaaacc atacgcgatg gtgtgcatca atattatcgc cgccgacagc aaccgcgacg
    37081 ctgaatttct gtttacctca atgcagcaag cctttgtgaa gctgcgccgt ggcgaaaccg
    37141 ggcaactgcc gccgccgatt caaaatatgg atcagttctg gtcaccgtct gagcagtatg
    37201 gcgtgcagca ggcgctgagt atgtcgttgg taggtgataa agcgaaagtg cgtcatggct
    37261 tgcagtcgat cctgcgcgaa accgacgccg atgagattat ggtcaacggg cagattttcg
    37321 accaccaggc gcggctgcat tcgtttgagc tggcgatgga tgttaaggaa gagttgttgg
    37381 gatagtgtgt cttaacgcgg gaagccttat ccgagctggc aacgctgtcc tacatagacc
    37441 tgataagcga agcgcatcag gcattgtgta ggcagcagaa atgtcggata aggcaccgct
    37501 gattactgat acaccggcag taaattaaag ctcgataaaa tatgcaccag tgcgttgccg
    37561 acgccaaaca ccagaatcag cgcaatcatc ggcttgccac cccagacgcg gaatttcggg
    37621 ctgccaaagc gtttacgcga tgcacgggct aacagcgccg gaacaattgc cgcccagatg
    37681 gtagccgcta aaccagcata accaatggcg tacaggaatc cgttcgggaa caacagcccc
    37741 cccacaactg gcggggcaaa ggtcagcaat gccgttttca agcggcccac agccgagtcg
    37801 tcgaaaccaa acagatctgc cagatagtca aacaaaccca gcgttacgcc gaggaacgaa
    37861 ctcgctaccg caaagtttga gaacacgacc agcagcagat ccagactacg gctgttcagt
    37921 acgccgctta acgcctgtac cagcacatca atattaccgc ccttctctgc aataccgata
    37981 aactccggac gcgggatgtt acccatcgtc gccagcaacc agatggtata cagcgccagc
    38041 gccatcagcg taccgtacac cagacatttc acgatggttt tcggatcttt gccgtaatac
    38101 ttcatcaggc ttggcacgtt accgtgataa ccaaacgatg ccagacagaa cggcagggtc
    38161 atcaacagat acggtgcata agacgcattg ctttcggcga cgttgaacaa tgtcgcaggc
    38221 tgcacatgcc ccagcaggct accaaaggtg aggaagaagg taatgacttt cgcccccagc
    38281 acaatcgctg tcatgcgact gacggcttta gtgctcaacc acaccacaaa cgctaccagc
    38341 aatgcaaaac caaaacccgc cgcccgtgcc gggacgttta gtgacatctc tgcgaaggtg
    38401 tgatgcagaa tcgaaccact ggcagaaata taggcatagg tcaggatata gagcacaaag
    38461 gcaatggaaa tgccgttgac cacgttccag cctttgccca gcaaatcttt ggtgatggtg
    38521 tcaaaactcg aaccgattct gtaattcagg ttagcttcca gaatcatcaa gccggaatgc
    38581 agcatacaga accaggtaaa gatcagcgcc gccattgacc agaaaaacca cgccccggac
    38641 atgaccactg gcagagaaaa catccctgcg ccaataatgg tgccgccgat aatcaccacg
    38701 ccgccaagca gcgacggtga cgtttgggtg gtggttagtg ttgccatgag ggcttctctc
    38761 cagtgaaaaa tagtgcgact gcgttgttat gcattgcact gtaccagtac acgagtacaa
    38821 aagacagaaa aaaagccccg atggtaaaaa tcggggctgt atatattatt ttacagattg
    38881 tgttcgctgt tcagcgatga ttacgcatca ccaccgaaac gacgacgacc ggtagaatca
    38941 tcacgacgcg gagcgcggcc ttcacgacgt tcgccgctaa aacgacgacc atcaccacgg
    39001 ccaccttcac ggcgttcacc gctgaagtta cgaccgcctt cacgacgttc gccaccgaaa
    39061 ccacgaccac cgccacgacg ctcaccgcca gtatgcggct gtgcatcgcc cagtaactgc
    39121 atgttcatcg gcttgttgag aatgcgagtg cgcgtaaagt gttgcagcac ttcacccggc
    39181 atacctttcg gcagttcgat ggtggagtga gaagcaaaca gcttgatgtt accaatgtaa
    39241 cggctgctga tgtcgccttc gttagcaatc gcaccaacga tatgacgaac ttcaacacca
    39301 tcatcgcggc ccacttcaat gcggtacagc tgcatatcgc caacatcacg acgttcacga
    39361 cgcggacgat cttcacggtc accacgcggg ccacggtcgt tacgatcgcg cggaccacgg
    39421 tcatcacggt cacggaattc acgtttcgga cgcatcggcg catctggcgg tacgatcaga
    39481 gtacgttcac cctgtgccat tttcagcagt gccgcagcca gagtttcgag atccagctct
    39541 tcaccttcag cagtcggctg aattttgctc agcagtgcgc ggtattgatc cagatcgctg
    39601 ctttccagct gctgctgtac tttagcggcg aatttttcca gacggcgttt gcctagcagt
    39661 tctgcgttcg gcagttctac ttccggaata gtcagcttca tagtacgttc aatgttgcgc
    39721 agcagacgac gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg acccgcacga
    39781 ccggtacgac cgatacggtg aacgtaagac tcagaatcca tcgggatatc gtagttaact
    39841 accaggctga tacgctcaac gtccaggcca cgggctgcaa cgtcggtcgc aatcaggatg
    39901 tccagacgac catctttcag gcgttccagt gtctgttcac gcagcgcctg gttcatgtca
    39961 ccgttcagcg cggcgctgtt gtagccgtta cgctcaagag cttcagccac ttccagagtc
    40021 gcgtttttgg tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc caggaaacgt
    40081 accagtgctt cgtttttgcg cataccccag acagtccagt agctctggct gatgtcagga
    40141 cgggtagtca cgctggactg aatgcgcact tcctgcggct ctttcataaa gtggcgggta
    40201 atgcgacgaa tcgcttccgg catggttgca gagaacagag cggtctgatg accttccggg
    40261 atctgcgcca taatggtttc aacgtcttcg atgaagccca tgcgcagcat ttcgtcagct
    40321 tcatccagaa ccagaccgct cagtttagag aggtccagag tgccacgttt caggtggtcc
    40381 agcagacggc ccggagtacc gacaacgatc tgcggcccct gacgcagggc gcgtaattgc
    40441 acgtcataac gctggccgcc gtacagagca accacattta cgccgcgcat gtgtttagag
    40501 aaatccgtca ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc cagcaccaga
    40561 atctgtggtg ctttcagctc aggatcaaga ttctgcaaca gaggtaaaga gaatgctgca
    40621 gtttttccgc tccccgtctg ggccataccc agaacgtcgc ggccattcag cagatgtgga
    40681 atacactctg cctgaattgg agatggtttt tcgtaaccca gatcgttaag ggcttcaagg
    40741 ataggagcct tcaggcccag atctgcaaaa gtggtttcga attcagccat gtagtacgtg
    40801 tgcctcaaaa ttaatggcgg ccagtctaca taactcatca tgaaattgat cagcaatttt
    40861 cattgaaaag tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac gccctcaccc
    40921 gttaaggtga tggcaatcaa aaaagattac gggctgatgt gtacgtcagc tattgctggt
    40981 ccgattctgc caggtcatct tggtcctggc ccaggagcga taattccaac aatgcgtatc
    41041 ggtgctcaac aaagttatga acgttgttgg caaccgccag tttgaacagt gccgtggcgc
    41101 tgtccaaatc ccccagactt aggtagtact tacctaaata gaagttggtt tcactgagat
    41161 gctcagcgag cgaggtgtta tccgttgcgt ccgccttgag cctttccatt aacgtttgtt
    41221 cgctaatgtt gcccaggtag aactcgacaa tgttccatcc ccactgttcc ttatccgatt
    41281 tttcgaagtg ctgtttcaac acttctttag cctgcttctc atcgagcttc tgctcggcga
    41341 gataaagcca cagactacgg aaaggatcat tgggatcgtc ttgataaaac gccagcagat
    41401 catcttgcgc taacttgtca cgaccgccgt aatataatgc gatcccgcga ttcaagtgcg
    41461 cgtagttgta agttggatca agctcaagta cagaatcaaa cgcttcatag gcagcatcaa
    41521 aattgcctgc ctgcgttaaa tatatgccta agtaattgaa tacttcaggc atatccggtc
    41581 ggattgccag cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg agactatcat
    41641 acaacactcc gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg
    41701 caaggatttg ttccatacgt gccagaatca cttcctgctg taaagtcggt tgcaatggta
    41761 ccgcgaggac ttcactttta cgccaggaag tattactgca tcctgcaagc gtaagtgctg
    41821 tcgcaacgaa acaccagcgc aaaaaaggct tcatttccca ctcccgaaga ccacggttga
    41881 atgaacgtcc tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg
    41941 cagcggaggg caaatggcaa ccttactcgc cctgttcagc agccggagct tccggtgctg
    42001 cagcaggttg agactgctca gtcgcttctt taatgctcag acggatacgg ccctggcgat
    42061 caacttccag aactttcacc ggtacttcct gacccatctg caggtaatcg gtcactttct
    42121 caacgcgttt gtcagcgatt tgagagatgt ggaccagacc ttctttaccg ccgccgatgg
    42181 caacaaatgc gccaaagtca acgatacggg tcactttacc agtgtagacg cggcccactt
    42241 cgatttctgc agtgatctct tcgatacgac gaatagcatg tttcgctttc tcgccgtcgg
    42301 tcgctgcaat cttcacagta ccgtcatctt cgatttcgat ggtggtgcca gtttcttcgg
    42361 tcagagcacg gattacagaa ccgcctttac cgataacatc tttgatcttg tccgggttga
    42421 tcttgatggt atggatacgc ggtgcgaact cagagatatc gccacgcggc gcgttgatcg
    42481 cctgttccat tacgcccagg atatgcagac gcgcaccttt agcctggttc agcgcaacct
    42541 gcatgatctc tttggtgata ccttcaattt tgatatccat ctgcagtgca gagataccgt
    42601 cgcgggaacc tgcaactttg aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt
    42661 cagacagtac aacgtagttg tcgccttctt tcaccagacc cattgcgata cccgcaacgg
    42721 cagctttgat cggcacacct gcgtccatca gcgccagaga cgcgccgcac acggaagcca
    42781 tagaagagga accgttggat tcagtgattt cagacacaac acgtacggtg tacgggaatt
    42841 tgtccatatc cggcatgact gccagcacgc cgcgcttcgc cagacgaccg tgaccaattt
    42901 cacgacgctt cggagaaccg accatgccgg tttcgcctac ggagtacgga gggaagttgt
    42961 agtggaacag gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac
    43021 gagcagtacc cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg aacagcgcag
    43081 aaccgtgagt acgcggcagc acgccagtac gcacatccag accacggatc atatcttttt
    43141 cacgaccgtc gatacgcggt tcgcctgcca gtacgcggct acgaacaacg tttttctcga
    43201 tcgcgtgcag aatttcaccc agttcgtttt cgtccagggt ttcgtcttca gcaagcagcg
    43261 tcgcgatggt ttcagatttg atgacatcaa cctgcgcata acgctcttgt ttgtcggtga
    43321 tgcggtaagc atcgctcagg cgagcttcag ccagtgcagc aacgcgcgcg tttagcgctt
    43381 cgtttaccgg ctccggctgc cagtcccaac gcggtttacc ggcttctttc accagttcat
    43441 tgatgttctg aataacaacc tgctgttgtt catgaccgaa cactactgcg cccagcatct
    43501 ggtcttcgct cagcagttga gcttcagatt caaccatcag tacagcggct tcagtaccgg
    43561 caacaaccag atccagtttg ctctctttca gctcgtcctg agtcgggttc agtacgtact
    43621 ggtcattgat gtaacctacg cgggcagcac caatcgggcc attgaacgga ataccagaca
    43681 gagacagcgc tgcggaagca ccaatcatcg cgacgatatc cgggttaact tgcgggttaa
    43741 cagaaaccac ggtggcgata acctgaactt cgttgacgaa gccttccggg aacagcgggc
    43801 gaatcgggcg gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttggg cggccttcac
    43861 gacggaagaa gctacccggg atacgaccag cagcgtaggt acgctcctga tagttaacgg
    43921 tcagtgggaa gaagtcctga cctagtttgg cttttttctg gccaacaacg gtaacgaata
    43981 ccgcggtgtc atccatgcta accataacag cggcagtagc ctgacgagcc atcatgccgg
    44041 tttccagagt cacggtgtgt tggccgtact ggaatttacg aacgatcgga ttaagcaatg
    44101 taatatcctt tctctttctt agacagtacc ttacggcact ggtgttaata cccgatcttc
    44161 tgcgcatcct cgcgactaat gacaacccta acccagctct atgtgggtaa agcctctcat
    44221 tagccgcgcg aacctctgca acggaagatc attcatagca acaatacatt agtttccagt
    44281 gaattgctgc cgtcagcttg aaaaaagggg ccactcaggc ccccttttct gaaactcgca
    44341 agaattagcg acgcagaccc aggcgctcga tgagctgggt gtaacgtgct acgtctttac
    44401 gtttcaggta gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga ccacgacggc
    44461 tgtggtgatc ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc tgtgcagtca
    44521 gcagtgctac ctgaacttcg gtagaaccgg tgtcgtttgc gtcacgacca aactcagaaa
    44581 cgattttagc tgttgcttca gtacttagag acattttaaa actccaaagt atatagaatg
    44641 aaaggacgcc gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt gttaaacaat
    44701 ttacgcgacg ttaagcggca gtattctact cgtagcgacc tgttatcgca agacggttaa
    44761 cattacgccg ggtattcaac caccaggcga cgaggcgcaa cgcggccttc atcgtcaatt
    44821 tcgcccatac cgataaattt gccgttctca ccttccgtga cgcgaaccag tccttccagt
    44881 ggcgcaccag atgtacgaac cgggttacca tttttgaagt aaacagaaga cgttaacgga
    44941 agattcacca ccggatagtc cgaagctgga ctgtccattg gcatcagtaa tggatcaagt
    45001 aactccgcgg ctggaatatc ctgctgttca gcttgctcaa caagttcacg caggtgctcc
    45061 agggtcacca tccgttcaac cggatattta cttaccgcca gacggcgcag gtaaataaca
    45121 tgcgcgccac agccgagttt ttcacccagg tcatcaatga tggtgcggat ataagtgcct
    45181 tttgagcagt gaatttccag ctccagctca ttgccttcat ggcgaataaa cagcaattca
    45241 taaacggtaa tcggacgcgc ttcacgcgga acttcaatgc cctgacgcgc atattcgtac
    45301 agttttttgc cctgatattt cagtgctgaa tacatcgaag ggatctgttc gatatcgcca
    45361 cggaaagtat ccagtgccgc tgccagctgc tctgcgctaa aggttaccgg acgttcttca
    45421 acgatctgtc cgtcggcatc agaagtatcg gtacgctgtc caagacgcgc aatgacccga
    45481 tagcgtttgt cggagtccag cagatactgg gaaaacttcg tcgcttcccc gaggcaaatc
    45541 ggcaacatgc cggtcgccag cgggtccagc gcaccggtat gcccggcacg gttggcgtta
    45601 tatatacgtt tcactttttg cagcgcatcg ttgctggaca taccctgagg tttatccagc
    45661 aacaaaacgc cgttaatgtc gcgaccgcga cgacgaggac gactcattag tcctcctcgt
    45721 cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca
    45781 tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca
    45841 ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga
    45901 tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat
    45961 acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat
    46021 ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct
    46081 gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc
    46141 cctttttaaa attaatttca ggtggaaggg ctgttcacgt tgacctgata agacgcgcca
    46201 gcgtcacatc aggcaatcca tgccggatgc agcgtaaacg ccttatcccg catggaaccc
    46261 taaaaacctt aagcaatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg
    46321 ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact
    46381 tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg
    46441 ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag
    46501 cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata
    46561 atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc
    46621 aggttataga tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt
    46681 gcagaggcat cagcacgtac gttaaagcca accaggatgg cgttggacgc cgcagccagg
    46741 gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg
    46801 tcagtagaca gtttcagcaa ggagtcggag atcgcttcga cagaaccctg tacgtctgcc
    46861 ttcaggacga tattcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg
    46921 agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg acgatagagt
    46981 gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt
    47041 acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga
    47101 cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg
    47161 cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg
    47221 aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga
    47281 acttccgcct gcagcaggat agcgtccagc agttcatcga taccggtacc cgctttcgca
    47341 gatacgtgta cgaactggct ttcaccgccc cactcttccg gcaggatgcc gtactgggag
    47401 agttcgttct taacgcgatc cggatcagct tctggtttat cgatcttgtt cactgcaacc
    47461 accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg cggcatcaca
    47521 ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc acgagcacgc
    47581 attgaagtaa acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt
    47641 tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggccactttc
    47701 gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc
    47761 acaaccggcg cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct
    47821 tccagctcgt tttcacgacg caggataact ttatggccca tctcttcagc aaccagctgt
    47881 gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct
    47941 ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt
    48001 tcgccgatca caacgtcacg gttaacggcc tgagcaggct tctggaagcc ttgctgcagc
    48061 gaagaacctt tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga
    48121 tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga
    48181 ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt
    48241 tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat
    48301 ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt
    48361 ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg ctcacggcgg
    48421 gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt
    48481 tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcggcctca
    48541 cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc
    48601 tcttccgctt cacgctgcgc ttgctcttcc gctgcaaggc gttcagcctc ttgcggatcg
    48661 cgtttcacaa aggtgcgttt cttgcggact tcgatttgta ccgatttgct ttttccaccg
    48721 gtaccaggaa tgttaagggt gctgcgtgtt ttacgttgca gcgtcaattt gtccgggcct
    48781 gaatttttct gattcaggtg gtcaatcaaa gtctgtttct cttgtgcaga cacagagtcg
    48841 tcagcagact tccggatacc tgcatcagca aattgctgta ccaggcgttc cacggaggtc
    48901 tgtcgctctg cggccagcgt tttaatcgtt acatctgtca tgctgttcct tcctgctaca
    48961 gtttattacg cttcgtcacc gaaccagcaa atattacggg cagccataat cagtgctccg
    49021 gctttttcgt cggtcaaccc ttcgatatca gccagatcat caatgccctg ttcggcgaga
    49081 tcttccagcg tacaaacgcc acgggcggcc agtttgaatg ccaaatcacg atctacccct
    49141 tcaaggttca gcagatcgtc agccggtttg ttatcaccga ggctttcttc ctgggcctgt
    49201 gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg cttcaacggt cggctcatca
    49261 aggccttcga tttccaacag ctctttcatc ggcacatagg ccagttcttc cagcgtcgag
    49321 aagccttctt ctaccagaac agtcgcgaag tcttcgtcga tgtcgagata tttggtgaag
    49381 gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct gcaggtcgtc aacggtcatc
    49441 acgttgagtt cccaaccgct cagctgcgaa gccagacgca cgttctgacc gttacggcca
    49501 atcgcctgcg ccaggttacc ggcttcaacg gcgatatcca tggtgtgttt atcttcatcc
    49561 accacgatag aagcaacgtc tgccggtgcc attgcgttaa tcacgaactg cgccgggtta
    49621 tcatcccaca ggacgatatc gatacgctcg ccacccagtt cagtagacac cgcctgaaca
    49681 cgcgcgccac gcatacctac gcaagcacct accggatcga tacgtttatc gttggttttc
    49741 accgcgattt tcgcacgaga acccgggtcg cgagccgctg ctttaatttc aatcacttct
    49801 tcgccgattt ctggcacttc aatacggaac agttcgatca gcatttccgg cttggaacga
    49861 gtgacgaaca gttgcgcgcc acgcgcttcc gggcgaacgg aatagagcac gccacgaacg
    49921 cggtcgccag ggcggaagtt ttcacgcggc agcatatctt cgcgcaggat cacggcttca
    49981 gcgttgttgc ccagatccag agagatgttg tcgcggttta cttttttcac cacgccggtg
    50041 atgatttcac cttcgtgttc acggaactga tcaaccacca tcgcacgttc ggcttcacgc
    50101 actttctgca cgataacctg ttttgccgtc tgggtagtga tacggtcaaa ggtaacagac
    50161 tcaatctgat cttcaacgta atcgcccagg ttcaggcttt catcttcata acgtgcggct
    50221 tcaagggtga tttccttggt cggctgggtg acttcatcaa caactaacca gcgacggaaa
    50281 gtgtcaaaat caccgctttt gcgatcgatc tgtacgcgga cgtcgatctc ttgttcatat
    50341 tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt cgaaaatctt ctcgcgaggt
    50401 agcgcctttt cattggatac ggcttcaact acagccaaaa tttctttgtt catcgcgggc
    50461 ttttcacctc atccagacta ttaaaagtgg ggaaccaggt tcgccttctg gatattactc
    50521 agcgcgaaca cttcatcttt accttcgacg gtaactgtga tcatttcacc gtctaccgct
    50581 ttgataacgc cctgccattt acgacggttt tgtaccgcca tacggagaac cagagtcacc
    50641 tcttctccga caaaacgggc gtagtgttca gccgtgaaca gtgggcgatc gagacccggt
    50701 gaggagactt ccaggttata agcaacggtg atgggatctt caacatccag cacagcactt
    50761 acctggtggc tcacatcagc acaatcatca acattgatgc catcttcact atcaatatag
    50821 atgcgcagtg tggatgtgcg accgcgaata aattcgatgc caaccagttc aaaacccagg
    50881 gcctcaactg gcgcagtaat catctctgtt aatttttgct ctaatgtgga caagcccacc
    50941 cccaagacat aaaaaaaggg cctaaagccc agttattctg tagtcagata acaaaaaacc
    51001 ccgataaatc ggggctttat ataactgaac cctataaccg caactgcggt ctggagcact
    51061 ttccagaagg attttttcaa atcccactac gaaggccgaa gtcttcacag tatatttgaa
    51121 aaaggactct aagggaaagt ggttgcgggg gccggatttg aaccgacgac cttcgggtta
    51181 tgagcccgac gagctaccag gctgctccac cccgcgcctg aaacgtggca aattctactc
    51241 gttttgggta aaaaatgcaa atactgctgg gatttggtgt accgagacgg gacgtaaaat
    51301 ctgcaggcat tatagtgatc cacgccacat tttgtcaacg tttattgcta atcatgtgaa
    51361 tgaatatcca gttcactttc atttgttgaa tacttttgcc ttctcctgct ctcccttaag
    51421 cgcattattt tacaaaaaac acactaaact cttcctgtct ccgataaaag atgattaaat
    51481 gaaaactcat ttattttgca taaaaattca gtgagagcgg aaatccaggc tcatcatcag
    51541 ttaattaagc agggtgttat tttatgacga cgattctcaa gcatctcccg gtaggtcaac
    51601 gtattggtat cgctttttct ggcggtctgg acaccagtgc cgcactgctg tggatgcgac
    51661 aaaagggagc ggttccttat gcatatactg caaacctggg ccagccagac gaagaggatt
    51721 atgatgcgat ccctcgtcgt gccatggaat acggcgcgga gaacgcacgt ctgatcgact
    51781 gccgcaaaca actggtggcc gaaggtattg ccgctattca gtgtggcgca tttcataaca
    51841 ccaccggcgg cctgacctat ttcaacacga cgccgctggg ccgcgccgtg actggtacca
    51901 tgctggttgc tgcgatgaaa gaagatggcg tgaatatctg gggtgacggt agcacctaca
    51961 aaggaaacga tatcgaacgt ttctatcgtt atggtctgct gaccaatgct gaactgcaga
    52021 tttacaaacc gtggcttgat actgacttta ttgatgaact gggcggccgt catgagatgt
    52081 ctgaatttat gattgcctgc ggtttcgact acaaaatgtc tgtcgaaaaa gcctactcca
    52141 cagactccaa catgcttggt gcaacgcatg aagcgaagga tctggaatac ctcaactcca
    52201 gcgtcaaaat cgtcaacccg attatgggcg tgaaattctg ggatgagagc gtgaagatcc
    52261 cggcagaaga agtcacagta cgctttgaac aaggtcatcc ggtggcgctg aacggtaaaa
    52321 cctttagcga cgacgtagaa atgatgctgg aagctaaccg catcggcggt cgtcacggcc
    52381 tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc gaaaagccgt ggtatttacg
    52441 aagctccggg gatggcactg ctgcacattg cgtatgaacg cctgttgacc ggtattcaca
    52501 acgaagacac cattgagcag tatcacgcgc atggtcgtca gttgggccgt ctgctgtacc
    52561 aggggcgttg gtttgactcc caggcgctga tgctgcgtga ctctctgcaa cgctgggttg
    52621 ccagccagat cactggtgaa gttaccctgg agctgcgccg tgggaacgat tattcaatcc
    52681 tgaataccgt ctcagagaac ctgacctaca agccagagcg tctgacgatg gaaaaaggcg
    52741 actcggtgtt ctcgccagat gatcgtattg gtcaattgac catgcgtaac ctggatatca
    52801 ctgatacccg cgagaaactt ttcggttatg ccaaaactgg cctgctttcc tcctctgccg
    52861 cttcaggcgt gccgcaggtg gagaatctgg aaaacaaagg ccagtaattc gacatcaacc
    52921 ctgcccttca ggggtggggt taccttgctt atcctctgta aactcctttc tgagcatcac
    52981 ataatcgaaa acatcatggt ttgcatccac aacccgtgag tctcctttat agtgtgcaat
    53041 cacttcctca agcgtttgcg gctgttccgg ctttgttacc cccatccacg cattaataag
    53101 gtaattattg taagctgtcg aaaagatgtt gttttccgtt gtgcgatcca cgccatcccc
    53161 aagaacgggg ctataccaga taaacatcgg gacatgatat gcctgctggc tagcctccct
    53221 gcctccatga aaatagacgt tcttcttcgt agggtcacgt tccaggccgt ggtcggcaaa
    53281 atacatgacc gaggcgcggc gatcttttaa taattcaaaa acctgaccta gcaaactatc
    53341 tgtgtaatga atggagttgt catagcaggc atcctgatcg tcctgcggtt gaaacacggc
    53401 gctggattgc ggataggcgc tacaagccgg ttcatggctt ccatttaaat gaagaacaat
    53461 cagtttcttt tgctgcgtat tttgctgtaa tgcttgcgat aaatgcggca acaacaattc
    53521 atcaaatcct ctgacataaa ctgtttccat ggcgcgcatg gcgatactgg taactgctgt
    53581 accattctgc cgaaaagcgg attgcgagct tagccagaaa gtctgaaatc ctgcttgatt
    53641 agccatatta ataatgttgt cggggtaatt atgaatgtca tgactcaaaa cagaatcagc
    53701 agttaaagaa aggggaaccg acagcgcagt gtaaggtgcg ccgcttattg cttgattaaa
    53761 cagtttgatc tgttttcttt gtgcttcaac ttgcggtgtc gtagagcgtg tatatccata
    53821 caaagacata ttgtcgacac gtacagactc ccccacaatc aacacgtagg tatcaatacc
    53881 tgtatccctg actgataatt gaaaatacgg caccgtgttt gcaattgaga gtaatctttg
    53941 atgctctttc gctgctaaag caaaatagtt gagattgaaa aacggcgtat aggtagcaaa
    54001 tcgcgacgct agtatatatg gactgaacgc ttttttattt tttgcatctt tataagcaaa
    54061 ttgacaagcg gaaaataaac tgcccgaaat gacaatcagc aataatattc ctgtcacttt
    54121 ttttgtcggc aaggaaacat catattttat tattactgcc aaaaaaagaa gggataaaaa
    54181 agcaaaggca catagataag ggatatacat ccccagcatt ttgacaactt catccggatc
    54241 actctggagc acactaatcg caaatccatc attgaaagtt gtaccaaagg tacaccatga
    54301 atagaggcta atactcatgt ctgccgcagt cagtaacaca aatggtgcgg caacaagcag
    54361 gcgaagcgga agggatgata tacgttttac aaccaggatg gtcagaacga agaacagcaa
    54421 gcgctgaacc atatgcgcgc cggaagaggc gactaagttc gtaataccaa aaacaataac
    54481 acaaagatac aacaaccaat gagatttaaa acttctagca aatttgttga atactgtcat
    54541 ccagaaatcc aagttcacaa ccaatataaa caattatcaa caatgttcaa ttttatatca
    54601 gacatgtttt tcaactctga tccatatcag accgaaacac tgttagtttt ttctttttaa
    54661 atgatgcggc cattgcgttc tcagtaatta aataacatta tcaatgcgtc tctaaacatt
    54721 acgcagaacg tagccatcag tagttatact gacaggagca ggatggttta tcgagaagta
    54781 aacatatccg tattcgtacc ggaactggag ttgacacaat aaagtgccaa ttatgtcagc
    54841 agaagggaaa agtttataac taaggcgtac cggcaccatc gtttcaaggt accagctacg
    54901 agtaaagcaa ctggacgaga tacagatacc tgacaaccat tcctcagacc aggaccaaaa
    54961 cgaaaaaaga cgcttttcag cgtctctttt ctggaatttg gtaccgagga cgggacttga
    55021 acccgtaagc cctattgggc actaccacct caaggtagcg tgtctaccaa ttccaccacc
    55081 tcggcacgga tactactttt tagttcggga tatcgctggt cggcttagcc ggagcagctg
    55141 gttgagtttg ttcggttttc accggtgcac tcagattttc ccattcgcta cctttattgg
    55201 ttttgttgct attgatgtta cccagcacca gactgatgat gaagaataac gttgccagca
    55261 gcgccgtcat gcgggtcatg aagttaccag aaccacttga accaaacagc gtagcggaag
    55321 cgcctgctcc gaaggaggct cccatatcag cgcctttacc ttgctgcagc atgatcagac
    55381 caacaaggcc aattgccaca ataaggaaaa ctactaaaag agcttcgtac ataatcaacc
    55441 tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa taaagcggga gtttttaatc
    55501 tttcccactg aagcgggtgt gaatactaac caaagcgaat gaccttcgca agggcaattt
    55561 tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg atgtgttcgc tgagaaaaaa
    55621 ggcgacaatc gccgcctttt tagccagtta tctaacgctt taaacggctt ttactgcatc
    55681 ggcgatgcgg tgtgcaaatt cagtcacctg cgcttcgtct tcgccttcca ccatcacgcg
    55741 aattaacggt tcggtgccgg atttacgcag caacacgcgt ccacggttgc ccagcgcagc
    55801 ttcaacctct gcggtcacgg ctttaactga ctcatgctca agtggatcgc cgctacctgc
    55861 ggtgtaacgt acgttaacca gaatctgcgg gaacattttc atgccgctgc aaaggtcgtg
    55921 caggctcata tggttacgtg ccatagccgc cagcacctgc aagccagcaa cgatgccgtc
    55981 accggtagta gttttatcca gcaggatcac atgaccggaa ttctctgcac cgatacgcca
    56041 gcctttctcc tgcatttttt ccagtacgta gcggtcaccc actttcgcgc gcgcaaatgg
    56101 aattcctaac tgtttcagcg ccagttcaag ccccatgttg ctcatcaatg tacccacagc
    56161 gccaccacgc agctggccct gacgaagacc ttcacgcgcg atgatataca tgatctgatc
    56221 gccatcgact ttattgcctt catggtcaac cataatcacg cgatcgccat cgccgtcgaa
    56281 ggcaataccg agatccgctt tttcagccag cacacgagcc tggagcgcgc gaacgtcggt
    56341 agcccccact tcggcattga tgtttacacc gtttggctca caaccgatag cgataacgtt
    56401 cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa gtcgcaccgt ttgcacaatc
    56461 caccacaatc ttcagttcac tgaggctaag ttcgttcggg aacgtggctt tgcaaaactc
    56521 gatatagcga cccgcggcat caacgatacg gctggcttta cccagttctg ccgaatcaac
    56581 gcagctgatc tccttttcca tttccgcttc gatggcctct tctaccgcat ccggcagttt
    56641 ggtgccgtcg atagagaaga atttaatgcc attatcgtag aacgggttat gcgatgcaga
    56701 tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga taagccacgg ccggtgttgg
    56761 catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc agacccgctt ccagtgctga
    56821 ctccagcata tagccagaaa tacgcgtgtc tttaccaata atgatcttac gggagccgtg
    56881 gcgcgccagc actttacccg cggcccaacc cagcttaagc acaaaatcag gtgtgatcgg
    56941 cgcatcccct acacgaccac gaatcccatc ggtaccgaaa tatttacgat tactcatagc
    57001 gtttgttttc ctttgcagac agagtggctt ccaccacccg catcgcttct acggtttctt
    57061 tgacgtcatg aacacgaata atgtgcgcgc cttgcattgc ggcaatgacc gcacaggcca
    57121 gactaccgct caggcgctcg gacggcccca cgttcagcag ctgcccaatc atcgattttc
    57181 gtgacatacc taccaacagc ggcaggttga aatggtgaaa ttcagccagg cgcgccagta
    57241 atgaatagtt atgggagaga tttttaccga aaccgaatcc ggggtcgagc aacaatttct
    57301 cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc aataaagtag cgattcactt
    57361 ctgcaaagac atcgtcatac ttcggagctt cctgcatggt ttttggattt ccctgcatat
    57421 gcatcagaca aaccggtaaa ccggtttctg cagccgcctc cagagcgcca ggttcggaaa
    57481 gggagcggat atcattaata atgtgagcgc caactttcgc tgactcacgg atgacttctg
    57541 gtttggatgt atctaccgag atccagactt cgaagcgttg agcaattgcc tcaaccacag
    57601 gaataacacg ttgcaactct tcttcaacgc taacttccgc cgcccctggg cgcgtggact
    57661 cgccaccaac gtcaatgatc gtcgcgccag cgttgatcat cagattcgca tgtttcaccg
    57721 catctatcag cgagttatgc gtgccaccat ccgaaaagga atcaggcgtg acgttgagga
    57781 tccccattac gtgaggatgg ctaaggtcca gtgaagtacc ctgggcaaag agtttcatga
    57841 tgttatccct ggtatgaatt gataagaaaa aaccccggag cacgccccgg ggttttcggt
    57901 acaaatacag tcatctgatg cgggaactta cttgtcgcct aactgctctg acatggtgtt
    57961 acccgggttc ggcgtacgcg gttcatcaac cggacgagga gcctttggac taccattgtc
    58021 gccagaattg ttagaagcgc ctggttcttc ccagcccgct ggcggacgta catcgcgacg
    58081 tgccatcagg tcatcaatct gcggtgcgtc gatagtctca tatttcatga gagcatcttt
    58141 catcgcatgc agaatatcca tattgtcggt cagaagctga cgcgcacgat tatagttacg
    58201 ctcaatcagt gctttcactt cctggtcgat gatacgtgca gtttcatcgg acatatgttt
    58261 cgctttcgct acgctacggc cgaggaatac ttcaccttct tcttccgcgt acagcagtgg
    58321 acccaatttc tcagagaagc cccactgagt caccatgtta cgtgccaggt tggtcgcaac
    58381 tttaatatcg ttggacgcac cggtagaaac atgttccggc ccgtagatga tctcttctgc
    58441 cagacgacca ccgtacagcg tagaaatctg gctttccagt ttctgacggc tggcgctgat
    58501 tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca cgaccgcgtg ggataatcgt
    58561 cactttgtgc accggatcgt gttccggcac caggcgaccg ataatcgcat gacccgcttc
    58621 gtggtaagcc gttgattctt tctgcgcttc cgtcatcacc atggagcgac gttccgcacc
    58681 catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc gacacaacgc gtttgttgcc
    58741 acgagcagcg aacagtgccg cttcgttcac caggttcgcc aggtcagcac cggagaaacc
    58801 aggagtacca cgggcaatga ttgccgcgtc gatatcgggt gccaatggta cgcgacgcat
    58861 gtgaactttc aggatctgct cacgaccgcg aacatctggc aagccaacca caacctgacg
    58921 gtcgaaacgg ccaggacgca gcagggccgg gtcgagaacg tccggacggt tagtcgcggc
    58981 gataacgatg ataccttcgt taccttcgaa gccatccatc tcaaccagca tctggttcag
    59041 agtctgttca cgttcatcgt gaccaccgcc cagaccagcg ccacgctggc ggcctacggc
    59101 gtcgatttca tcgataaaga tgatgcacgg tgccgctttc ttcgcctgtt cgaacatgtc
    59161 acgaacacgg gatgcaccca caccgacgaa catttctacg aagtcagaac cggagatagt
    59221 aaagaacgga actttcgctt cgcctgcaat cgctttcgcc agcagcgttt taccggtacc
    59281 cggaggaccg accatcagga cgcctttcgg gatcttaccg ccgagtttct ggaagcggct
    59341 cggctcgcgc agatactcaa ccagttcagc aacttcttct tttgcttcgt cgcagcccgc
    59401 aacgtcagca aaggtcgttt tgatctgatc ttccgtcaac atgcgcgctt tgctcttacc
    59461 aaacgacatg gcacctttgc caccgccgcc ctgcatttga cgcatgaaga agatccagac
    59521 accaatcagc aacagcatcg ggaaccagga gatgaagata gaagccagca ggcttggttc
    59581 ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac aggttatcca gtaatttcgg
    59641 atcctgaacc ggaatgtaag tggtataacg gttactatct ttcttggtaa cgttgatttc
    59701 acgtccgttg atacgcgctt cacgaacctg gtcgttattg acctcttgta ggaaggtaga
    59761 gtaatccacc ttacggccat tagactcgct gggcccaaag ctctggaata ctgacatcag
    59821 cacaacggca atgaccagcc agagtattag gtttttcgcc atgtcactca agggattaac
    59881 ctcttattac aactgtgtta aaaacagcgt caggatactc tatatccagc atctttcaaa
    59941 ctttcgtctg aaatctcccg gttagggttt acgcccggtc gctacaatat acacttcccg
    60001 cgaacgtgca cgagaagagt ccggcttacg aactttaacc ttcgtaaaca gggagcgaat
    60061 ttcccttaga tactcatcga aaccttcgcc ctggaacacc ttcactacaa aactgccacc
    60121 tggcgctaat acatcacgac acatttctag cgccagttcc accagataca tggcacgggg
    60181 gatatccacc gccggtgttc cgctcatgtt tggtgccata tcggacatga caacctggac
    60241 tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc agttcatcac gaaaatcgcc
    60301 ctgaagaaag tccacaccaa cgataggatc cataggtaaa agatcgcaag cgatgatgcg
    60361 gcctttgccg ccaatttggg tgaccacata ttgtgaccaa ccacccggag cagcaccaag
    60421 gtcgacaacc gtcattcccg gtttaaagag tttgtcactt tgctgtattt catcaagttt
    60481 aaaccaggca cgggaacgta accccttttt ctgtgcctgt tgaacatatt tatcgctaaa
    60541 gtgttcctga agccagcggc tggagctggc agaacgcttc ttacctgtca tttaactttc
    60601 ccatggggat aactcatcgt aaccaattgc gtaaattttt acgcgcctat ttggtgatat
    60661 atgggagatg gcggtagaat gacccgtttt caatcccaac gtaagcaaaa aatacgatga
    60721 atctgagtac taaacaaaaa cagcacctga aaggtctggc acatccgctc aagccagttg
    60781 ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc cgagattgaa caagcgttag
    60841 agcaccatga actcatcaag gtgaaaatcg ccaccgaaga tcgcgaaact aaaaccttga
    60901 tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt acaggtcatc ggtaaaacgc
    60961 tggtgcttta tcgcccaact aaagaacgta aaatctcgct gccacgctaa gattatccta
    61021 aagttacaca catttgctgt gtaaaacgag gggttttccg caggcaggag agcaaaatgc
    61081 cacgctctgt tcgttgataa aaggccgcat agcggccttt ttcctttctt tacaatacat
    61141 caacatcttg agtattgggt aattcttaca ggtattccac cttaattact tcaaattcta
    61201 cttcgccgcc cggcgttttg atgaccacaa catcatcttc ttctttgccg atcaggccac
    61261 gagcaatagg cgagtttaca gaaatcaggt tttgtttaaa gtcagcttca tcatcgccaa
    61321 cgatgcgata agtctgttct tcgtcagaat cgagattcag caccgttacg gtagcaccaa
    61381 aaataacgcg cccattgtta ggcattttgg tgacatcaat cacctgcgcg tttgacagct
    61441 tggcttcgat gtctttgata cggccttcgc agaagccctg ctgttcacga gctgcgtggt
    61501 attcggcgtt ttctttcagg tcgccatgct cacgcgcttc cgcgatagca gcaatgattt
    61561 caggacggcg cacagatttc agaaaatcca gctcttcgcg taatttttct gcgccgcgta
    61621 aggtcatcgg aatagcttgc atttgttata cctcttgaat attcctgatg gggcaagtct
    61681 tcacccaccc cggctgttcg gcctgcccgg cataactcct caccgggacc agaagcaaaa
    61741 aaataccgac ccgggtacaa gtcccaggtc agctacaatt cacattttga tagtcatttt
    61801 accctgaagt tcccgaaggg tcatcgttta ctttataggg cgttgcgccg tagtatgacg
    61861 gctcgattcc aggttgttag cgcgagatta tgcgattttc cagatttatc atcggattga
    61921 ccagctgtat agcgttcagt gttcaggccg caaatgttga tgagtacatt actcaactcc
    61981 ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg
    62041 attaccacag tcagcagatg gcgctgcctg ccagtaccca gaaagtgatt actgcgctgg
    62101 cggcgttgat tcaactcggc cccgattttc gttttaccac gacgcttgaa accaaaggca
    62161 atgtggaaaa cggcgtactt aagggtgact tagtggcgcg atttggtgcc gatccgacgt
    62221 taaaacgtca ggatattcgc aatatggtcg cgactttgaa aaaatctggc gtcaaccaaa
    62281 tcgatggcaa tgtgttgata gatacctcca ttttcgccag ccacgataaa gcccccggct
    62341 ggccatggaa tgacatgaca caatgcttta gcgctccgcc tgccgccgcc atagttgacc
    62401 gcaactgttt ctccgtctcg ctctacagtg ccccaaagcc tggtgatatg gcttttatac
    62461 gcgtggcatc ttattacccc gttacgatgt tcagccaggt acgcaccctc ccccgtggtt
    62521 ctgccgaagc gcaatactgc gaactggatg tggtgccagg cgacctgaac cgctttacgc
    62581 tgacgggatg cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg
    62641 gagccagcta tgccggtgca attctgaaag atgagttaaa acaggcgggt atcacctgga
    62701 gcggaacact gctgcgccag actcaggtta acgaacctgg aacggtagtt gccagtaaac
    62761 agtcggcccc gctgcacgat ctgcttaaga ttatgctgaa aaagtcggac aacatgatcg
    62821 ccgatacggt tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga acatggcggg
    62881 ccgggtcgga cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat attggaaaca
    62941 ccattattgc cgatggttca gggctttcgc ggcataacct gattgccccc gccaccatga
    63001 tgcaggtgct gcaatacatt gcccaacacg acaatgaact taactttatc tccatgctgc
    63061 cgctggcggg ctatgacggc tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg
    63121 atggaaaagt ctcagcgaaa accggttcgt tgcagggggt atataacctg gcgggattca
    63181 ttaccacagc gagcgggcaa cgaatggcgt ttgtgcaata tctttctggc tatgcagtag
    63241 aacctgcgga tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata
    63301 aagatattta tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg gggcttcaga
    63361 ttattgacaa agtgcgcttt gtttatgccg gatgcggcgt aaacgcctta tccggcctac
    63421 aaaatcgtgc aaattcaaca tattgcaatt ctcttgtagg cctgataagc gtagcgcatc
    63481 aggctgattt ggcgtttatc atcagtgatt aacgcttgta aatgaactca acgccttctt
    63541 cgtcgtcttc gtcccagtcg tcatcccagt cttcatcatc ctcttcagca atctcttcaa
    63601 gctgctggcg atgataatca tcccacatga attcgacttt ctctggctgt ttcgcttctt
    63661 cagcctgcac gaccgggttt tcaatgataa aggtcatcac atcccagcag agatctttca
    63721 cgcccagtcc actcgccgca gagatcagat aatatttatc ttcccagccc agcgcctcag
    63781 cgatcgcttt cgctttctct tcggcttcta ccttatccag cagatcgatc ttgttgaaca
    63841 ctaaccaacg cggtttcgcc gccagatcct ggctgtattt ttccagctcg ctgataataa
    63901 tacgcgcgtt ttcaaccgga tcggtgccgt caatcggatc gatatcgatg aggtgcaaca
    63961 ggacgcggca acgttccagg tgcttcagga agcgaatgcc cagacctgcg ccttccgcag
    64021 cgccttcaat cagtcctgga atatcggcaa caacgaagct cttttcgttg tccattcgta
    64081 ccacacccag acttggcacc agagtggtaa acggataatc cgccactttc ggtttagccg
    64141 ccgataccgc acgaataaag gtcgatttac ccgcgtttgg catccccaac ataccgacgt
    64201 cagccagcag catcagctcc agcagcagct cgcgcttatc gcccggcgtg ccgttggttt
    64261 tctgccgcgg tgtacggtta acggacgatt tgaaacgggt attgcccaga ccgtgccagc
    64321 cgcccttagc aaccagcaga cgctgaccgt gtttggtcat atcgcccatg gtttcaccag
    64381 taccctggtc gattacacgc gtacctaccg gcactttaat tgtgacgtct ttaccgcgct
    64441 taccggtaca gtcgcggctt gcgccgttct gaccgcgctc tgcacggaaa gatttttcaa
    64501 aacgataatc gataagcgtg ttcaggttct cgtcggcttc catccatacg tcaccaccat
    64561 caccgccgtc gccgccatcc gggccgcctt tcggaatata cttttcgcgg cggaagctca
    64621 cgcaaccatt accgccatca cctgcaacga ccagaatcga tgcttcatca acaaacttca
    64681 ttttattctc cgtaaatcat tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac
    64741 gtaatccgcc ccaaatacga tgaccaatgg cggaatacat cgcgcccgca accacgacaa
    64801 acgcaccgag ataacctaaa aggtttaaca tcggtctggc gaagaaatcg ggccaggcca
    64861 gtgataaaag atctgaaaaa aacagcgtaa acagtggggt gagcgtgatg atcgcgctca
    64921 cctgcgctgc ctgccagcga gccatcgctt ccgccagggc gccatatcct accaatgtat
    64981 tcagtccgca aaaaattaaa catgcgagct gccagtggct aagctgcgct atcactccag
    65041 gctttgccag agggaagagc gcaattgtac ataaagtgta cagtaaaaac aggatctgcg
    65101 gtgaggccag ccgacgcaat aaaacctttt gcgccacgcc ataactcacc caaaccgtcg
    65161 cagcaccgac cccaaagata actccccagg tgtaatcggt gagctttgta aatatctcga
    65221 ccagactggt gttaaaaaac atcaccaggc cgctcaggag catcaatgcc cctacaacct
    65281 gagtgctgcg cattttctct ttcaggataa atacgctggc aaccatcatg ccaactggcg
    65341 agagttgccc aatcacctgc gaagcggtcg gactcaggta ttgcaaggat gagctgaaca
    65401 ggatgaagtt cccaaacagc ccggcggtcg ccactgccaa caaaatcaac cagcgtggct
    65461 tacgaaacac gcgtaatggc ggcaacctct tcttcaccgc aagaatggca cccaggccaa
    65521 tactcgccat caagaaacgg taaaacacga ttgtcggagg ttccatcacc tccagcacct
    65581 gcttcattgc gattggcaac gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc
    65641 caatgcctgc ctgctgcttc ataccgtatt ccctgcaaag cgcatttgcg gattaacgtc
    65701 gcaccatgcg catttttacc ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc
    65761 tttcatccgt taccgggacg cgaaaaactt attcagcttc gatgctgata aatttacggt
    65821 ttttcgggcc tttaacttcg aatttcactt taccgtctgc tttagcaaac agagtgtggt
    65881 cacgaccgca acctacgtta gcgccagcgt ggaatttggt accacgttga cgaacgatga
    65941 tgctacccgc cagaacggat tcgccaccga aacgcttaac gcccaggcgt ttagcttctg
    66001 aatcgcgacc gttacgtgtg gagccgccag cctttttatg tgccatttga aatctctcct
    66061 caggtcttag gcgctgatgc cagtaatttt cacatcagtg aaccactgac gatggccctg
    66121 ctgcttacga tagtgtttac gacgacgaaa cttaacgatt ttaactttct cgccacgacc
    66181 gtgagcaaca acttcagctt tgattacgcc gccatcaacg aaaggaacgc cgattttgac
    66241 ttcttcaccg tttgcgatca tcagcacttc agcgaactca acagtttcgc cagttgcgat
    66301 gtccagcttt tccaggcgaa cggtctgacc ttcgcttact cggtgttgtt taccaccact
    66361 ttggaaaacc gcgtacatat aaaactccgc ttccgcgcac accttttcaa tgattcagag
    66421 tgcgctataa atattcacaa tagggcgcga atattacgca aaacgcacgc ctttgacaag
    66481 tgctacagtc aatacacgaa gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc
    66541 attttttcag tacaatcacc ctatattcct aaccataaac cctaagttgc ctttgttcac
    66601 agtaaggtaa tcggggcgaa aagcccggct tttgcgatga atttagaaaa aatcaatgag
    66661 ttaaccgcgc aagatatggc gggtgttaat gcggcaatcc ttgagcagct taattccgac
    66721 gtccaactga tcaatcagtt aggctattac atcgtcagcg gcggcggtaa acgtattcgt
    66781 ccgatgattg ctgtactggc tgcacgagct gttggctatg agggaaatgc gcatgtcacc
    66841 attgctgccc tgatcgagtt tatccacacg gcgactctgc tgcacgacga cgttgtggat
    66901 gaatcagata tgcgcagggg taaagctacc gccaacgccg catttggcaa tgccgccagc
    66961 gtgctggtag gcgattttat ttatacccgc gctttccaga tgatgaccag cctcggttca
    67021 ctcaaagtgc tggaagtcat gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg
    67081 caactgatga atgttaacga tccggacatc actgaagaaa actacatgcg cgttatctat
    67141 agcaaaaccg cgcgtctgtt tgaggctgcc gcgcagtgtt ccggaattct ggctggctgt
    67201 acgccggagg aggagaaagg cctgcaggat tatgggcgct atctcggcac tgctttccag
    67261 ttgatcgacg atttactcga ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc
    67321 gacgatctga acgaaggtaa accgacgctg ccgctgctgc atgcgatgca tcatggcaca
    67381 ccagaacagg cacagatgat ccgtaccgcc atcgaacagg gtaacggtcg ccatcttctg
    67441 gaaccggttc tggaagcaat gaacgcttgt ggatctcttg aatggacgcg tcagcgcgcc
    67501 gaggaagaag cagacaaagc catcgcagcg ttacaggtgc tcccggacac cccttggcga
    67561 gaagcactca tcggcctcgc gcacatcgct gttcaacgcg atcgttaatc ccctcccctc
    67621 atcccgcgca gtgcgcggga tgattccagt aaactccata agaatactta ttcagcgtaa
    67681 taatgcactg aaaaaccagt gtatacatct ttatatattc tgaatattca cactctttac
    67741 aggaactttt tagagcaatc ggccatcagg agtatagtga tgctcgacag aagaagtgtt
    67801 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    67861 catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggaatct
    67921 cgcagaaatg gtttgagttc ctcaacgctg gcgaatgcat tatcgcgccc atggccgaaa
    67981 ggagagatga ttattgcgaa ggccctggga actgacccct gggttatctg gccatcacgc
    68041 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    68101 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgtac ggggctgcca gctttcagac
    68161 gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg
    68221 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    68281 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    68341 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc gtgcgcgccc
    68401 atacggctca gctctggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    68461 ccagtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    68521 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    68581 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    68641 gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc
    68701 gccaccagga atgtaccggt ttcgatacga tccggcagca cgcgatagac accgccgcct
    68761 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    68821 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    68881 atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    68941 actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa
    69001 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    69061 ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    69121 accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    69181 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    69241 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagtttcgg gacgttctgg
    69301 atctctaccg gttcttccgc cagtagtgcg gcaaaaagga taggcagagc agcattttta
    69361 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    69421 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    69481 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt aatcgacaca
    69541 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    69601 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    69661 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    69721 ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa
    69781 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    69841 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    69901 agaacatcag caggcaaatt atacaatttc gccagggtat acactttgtc gtttaccccc
    69961 tgaagcgtca cattgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    70021 agtccccccg tatccacgcg ggagacacgg ctaagatcga tgcaggtaat ccccttcacc
    70081 gcttcctcac gcatttccca aagcggtagc aaaacgtcct gatccagctc tccggataac
    70141 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    70201 gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg
    70261 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    70321 tcatgtcgta agcctgccaa ttgcccgtct gggagttttt acgccactgg aagtccagac
    70381 gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat
    70441 cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    70501 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag
    70561 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    70621 atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    70681 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttatacg
    70741 gattggtctg gtctgccgcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca
    70801 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    70861 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    70921 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag
    70981 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    71041 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    71101 tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    71161 ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg
    71221 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt
    71281 cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg
    71341 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    71401 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    71461 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    71521 aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc
    71581 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg
    71641 cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc
    71701 gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc
    71761 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    71821 tctgcggtta acgccgaacc agcacgcccg gcaaacaaca gcgcggcaac caccggcccc
    71881 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    71941 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    72001 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt
    72061 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    72121 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    72181 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    72241 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    72301 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    72361 aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    72421 gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa
    72481 aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg
    72541 ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac
    72601 ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggaagtt gggtatgttc
    72661 gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    72721 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    72781 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    72841 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg
    72901 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    72961 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    73021 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg
    73081 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    73141 ccgttacggc tttctgaaaa tcttcagcgg accggcgagt atacctgaag aaaggacgtt
    73201 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    73261 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    73321 tcggcatgac ggtggtcagt attggtacat cgttaccaga agtcatcgtc tcgcttgccg
    73381 cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca
    73441 atatattgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattctgatg
    73501 ttctacgccg tgaattaccc ttaatgttgt tggtcagcgt ggtggccggt tccgtactct
    73561 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    73621 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    73681 gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg
    73741 gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc
    73801 tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa
    73861 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    73921 ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg
    73981 cgctgataac gccaggagag attgatccac tggcgtacag tcgtgactac agcgtgatgt
    74041 tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg
    74101 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    74161 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    74221 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    74281 cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa
    74341 gggaaagttg tcgtcatggg gatgggaaaa tcggggcata ttgggcgaaa aatggcggca
    74401 acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    74461 gatttaggca tggttacccc acaggatgtg gtgattgcta tctctaactc tggtgaatcc
    74521 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    74581 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    74641 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt
    74701 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    74761 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    74821 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcgttg
    74881 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    74941 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    75001 cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg
    75061 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    75121 ggcgaccatt tactcggtgt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    75181 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    75241 ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg
    75301 atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt
    75361 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    75421 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    75481 acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg gaaaaactgg
    75541 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    75601 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    75661 attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac
    75721 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    75781 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcatta atatggccga
    75841 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    75901 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    75961 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    76021 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    76081 ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag actctcaact
    76141 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    76201 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    76261 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    76321 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    76381 catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc
    76441 ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca
    76501 tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag
    76561 tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg
    76621 ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg
    76681 gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg
    76741 tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaaca accgttctgg
    76801 tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta
    76861 attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc
    76921 gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc
    76981 caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg ccgcgcgatg
    77041 cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc
    77101 gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg
    77161 ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc
    77221 gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt
    77281 cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga
    77341 aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta
    77401 aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact gaccacaacg
    77461 tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg
    77521 cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg
    77581 aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct
    77641 gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct
    77701 ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca
    77761 ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg
    77821 cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc
    77881 ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg
    77941 caccagcggt gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac
    78001 cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc
    78061 tattgctacc tctatcgtcg atgccgttga tgacaccggt tatctgactg tcccgctgga
    78121 agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct
    78181 taagcggatc caacggtttg atccggtcgg tgtggcggca aaagatctgc gtgactgtct
    78241 gctgatccaa ctctcccaat tcgataagac cacgccgtgg ctggaagagg ccagactgat
    78301 cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac
    78361 gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg
    78421 ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg
    78481 taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat
    78541 caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat
    78601 ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac
    78661 gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggtga
    78721 agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc
    78781 gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact
    78841 gaagtatttc ttttccagtc acgtcaatac cgagggcggc ggcgaagctt cctccacggc
    78901 gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga
    78961 cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc
    79021 gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacaac tcgtttgacc
    79081 caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg
    79141 aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa
    79201 tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac
    79261 tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca
    79321 ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac
    79381 aacactaatt gtccgggcaa ttagcatgtg catggcggtc tgttgtgcgg cacaacgggc
    79441 catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg
    79501 aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta
    79561 cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg
    79621 cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa
    79681 aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag
    79741 atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca
    79801 tcgacaacca accggtggac ttactttttg ccctgctggt gccggcagac caaactaaaa
    79861 cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc
    79921 gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta
    79981 ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt
    80041 acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg
    80101 cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc
    80161 tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata
    80221 tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac
    80281 cgcaactcct gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc
    80341 gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa
    80401 gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt
    80461 ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac
    80521 tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg
    80581 tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag
    80641 gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct
    80701 accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct
    80761 acttgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc
    80821 aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg
    80881 aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca
    80941 tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctctt
    81001 acgtaatgac gaaggcaccg aggctgaagc caacagcgtt attgcgctgc tgatgttgga
    81061 ttctgccaaa ggacggcaga ttgaagttga agcgaccggt ccacaggaag aggaagcact
    81121 ggccgccgtt atcgccctct ttaattctgg ttttgatgaa gattaatctt catcactttg
    81181 acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct
    81241 caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac
    81301 cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa
    81361 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    81421 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    81481 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    81541 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    81601 cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaagcctt ccattacgct
    81661 gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact
    81721 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    81781 gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat
    81841 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    81901 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    81961 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg
    82021 gtgttgttgc ataaacggct caccgcctaa ctgatacatc ttccgtaaaa tccacgcctg
    82081 acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac
    82141 agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    82201 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    82261 cgtcaggata cgctttttgc tccagaccgt ttctatcccc agcgttaatc cggcttccag
    82321 cccttttcga acccagctac gcccatccca taaaaagaga tttttggctg tctgttgaga
    82381 aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc
    82441 aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac
    82501 cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta
    82561 acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg
    82621 aacaggcgca acgctaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat
    82681 taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca
    82741 gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc
    82801 tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca
    82861 tcgacaggac acggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata
    82921 tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca
    82981 atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat
    83041 tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg
    83101 ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga
    83161 cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc
    83221 gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc
    83281 gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa
    83341 ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt
    83401 tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa
    83461 ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac
    83521 agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg
    83581 ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac
    83641 ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc
    83701 accgacgtta tcttcccagg ccggaaggtc aggagactga atttgctgac ccagctgttg
    83761 cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat
    83821 gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat
    83881 cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg
    83941 ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt
    84001 actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac
    84061 agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt
    84121 cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgtttcgt
    84181 cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca gcaacaccag
    84241 gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac
    84301 gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc
    84361 caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac
    84421 ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc
    84481 gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc
    84541 aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc
    84601 gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg
    84661 cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt
    84721 gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac
    84781 ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg
    84841 caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc
    84901 aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc
    84961 cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc
    85021 ggtatccagc agaatgcggc tcagaccgac gataccgttc agcggtgtac gcaattcgtg
    85081 actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata
    85141 ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg
    85201 gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag
    85261 ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac
    85321 ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc
    85381 gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt
    85441 acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg
    85501 ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt
    85561 ttcctgcagt tctgcttccg ctttttcacg aacggcaatt tcctgattta gctgggcaat
    85621 attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg
    85681 caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa
    85741 gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct
    85801 ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac
    85861 gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac
    85921 ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc
    85981 ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc
    86041 tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca
    86101 tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacagc
    86161 ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt
    86221 ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc
    86281 aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg cagcggatgc
    86341 agctttatgc cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa acattcagcc
    86401 tgcccttcgc caggcaggcc gacaattaag tgggaacaca ccttcagccc gcgctgacgt
    86461 gccagctggg ttgtacgctg ataacaggca aaatcatggc cgcggttgat gcgatgcagt
    86521 gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtaacc ctggtcctta
    86581 tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt acccacacac
    86641 aaaccgacaa tattggcctg gctcaccgcc tgctgataca tagaacgcag cacctgaact
    86701 tccgcaaagg tgctggtata cgcctgaaag taggccagat agcgtttagc gcggttaact
    86761 aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca
    86821 aacgaggcaa cattacagaa tgtgcagcca ccacgcccga tggtaccgtc acggttaggg
    86881 cagctaaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga
    86941 tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga
    87001 aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc
    87061 ggcctgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca
    87121 taaatcactt ttgcttatct tgtgtcagat ttttttatct cctgatggat tttaggcaaa
    87181 aacagtagca tgaaacgtca ttaccaatta aggcagtata aaatgctggt tttgtcgtca
    87241 gttcaaggca ggataagggt taacacacct ttatgacagt caggaattga ctgtttctct
    87301 aacgacttcc cttttagcct taaagataaa atccatttta atttcagtca tttaataaag
    87361 aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt
    87421 tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt
    87481 atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg
    87541 gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt
    87601 gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta
    87661 cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga
    87721 acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg
    87781 cgcgattctc gccgatggta aaaccggcga cggttgcggc ttgctgttac aaaaaccgga
    87841 tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa actacgctgt
    87901 cgggatgctc ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga
    87961 agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga
    88021 aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa tttttgtgaa
    88081 cgccccggca ggctggcgtc cacgcgatat ggagcgccgt ctgtttatcg cccgccgccg
    88141 cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt
    88201 gaacatctat aaaggtctgt gtatgccgac ggatctgccg cgcttttatc tggatcttgc
    88261 ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca ctaacaccgt
    88321 accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg aaatcaacac
    88381 catcaccggt aaccgccaat gggcgcgtgc gcgtacttat aaattccaga caccgcttat
    88441 ccctgacctg cacgacgccg caccgttcgt caacgaaacc ggctctgact ccagttcgat
    88501 ggataacatg ctggaactgc tgctggcagg cgggatggat atcatccgcg ccatgcgtct
    88561 attagtacca cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt
    88621 tgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga
    88681 cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc gctacgtcat
    88741 caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga
    88801 cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg acacccgcag
    88861 tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa
    88921 agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga
    88981 agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta
    89041 cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt
    89101 cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta
    89161 cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga
    89221 agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc
    89281 agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa
    89341 acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga
    89401 cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat
    89461 ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct
    89521 gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct
    89581 gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc
    89641 cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg
    89701 ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg
    89761 taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc
    89821 ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct
    89881 gtgcttccag ggggcggtca gccgcattgg tggggcaagc tttgaagact tccagcagga
    89941 tctgctgaat ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc agggcggcct
    90001 gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct
    90061 gcaacaagcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa
    90121 tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt
    90181 caacattgct gatgttgaac cggcaagcga actgtttaaa cgctttgata ccgccgcgat
    90241 gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat
    90301 cggcggtaat tcgaactccg gtgaaggcgg cgaagacccg gcgcgctacg gcaccaacaa
    90361 agtgtcgcgc atcaagcagg tggcttccgg tcgctttggg gttactccgg cgtatctggt
    90421 caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccaggcg aaggcggtca
    90481 gttgccgggt gataaagtca cgccttacat cgccaaactg cgctattcgg tgcccggagt
    90541 gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct
    90601 cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga
    90661 accgggagta ggcaccatcg cgactggcgt ggcaaaagct tatgcggact tgatcaccat
    90721 cgcaggctat gacggcggca ccggcgcaag tccgctttca tcggtgaaat acgcaggctg
    90781 tccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgctaacg gcttgcgtca
    90841 caagatccgt ttgcaggtcg atggcggcct gaaaacgggt gtcgatatca tcaaggcggc
    90901 gattctcggc gcagaaagct tcggcttcgg cactggcccg atggtggcgc tcggctgtaa
    90961 atatctacgt atttgccatc tgaacaactg cgcaacgggt gtagcaactc aggatgacaa
    91021 actgcgtaag aaccactatc acggcctgcc attcaaggtg acgaattact ttgagtttat
    91081 cgcccgtgaa acccgcgagc tgatggcaca gcttggcgta acacgtctgg tggatctgat
    91141 tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc agaagctggc
    91201 gctgtcgaag ctgctggaga ctgccgaacc gcatccaggt aaggcactct actgcaccga
    91261 aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc
    91321 gtttgtcgat gagcgccaga gcaaaacctt ctggttcgat attcgcaaca ccgaccgttc
    91381 tgtcggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc
    91441 cgatcctatc aaagcgtact tcaacggcac cgcaggccag agcttcggcg tgtggaacgc
    91501 gggcggcgtg gaactgtacc tgaccggtga tgccaacgac tatgtcggta aaggcatggc
    91561 gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag
    91621 cattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg
    91681 cgcgggtgaa cgtttcggcg tgcgtaactc cggtgctatc accgtggtag aaggcattgg
    91741 cgacaacggt tgtgaatata tgacgggtgg tatcgtctgc attctgggta aaaccggcgt
    91801 taacttcggt gcgggcatga ccggcggttt cgcttacgtt ctcgatgaaa gcggcgattt
    91861 ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgctc tggcgatcca
    91921 tgaagagcat ctgcgtggtc ttatcaccga gcatgtgcag cataccggct ctcagcgcgg
    91981 tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa
    92041 gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt agcgcagctg agttgcgcgt
    92101 gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca
    92161 gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta
    92221 cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa
    92281 cccatactgc gagtggaaat gcccggtaca caactacatc ccgaactggc tgaagctcgc
    92341 caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga
    92401 agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga
    92461 tgagtttggc gcggtgacca tcggcaacat tgagcgctat atcaacgata aagcgttcga
    92521 gatgggctgg cgtccggata tgtctggtgt gaaacagacc ggtaaaaaag tggcgattat
    92581 cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc
    92641 cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt
    92701 caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga
    92761 attcaaactc aataccgaag tgggccgcga cgtacagctg gacgatctgc tgagtgatta
    92821 cgatgccgtg ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga
    92881 agacgccgat ggcgtgtacg cagcgctgcc gttcctcatc gccaacacca aacagttaat
    92941 gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt
    93001 ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa
    93061 gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt
    93121 gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccgc tgggtattga
    93181 agtgaacggt aacggcaaag tcagcggcgt aaaaatggtg cgtaccgaaa tgggcgaacc
    93241 ggacgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc
    93301 agatgcggtg atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca
    93361 cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt
    93421 ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt
    93481 ggtgaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt
    93541 ttaagcgagg taacaatgaa ttcgccagac tgtgtctggc gaatcaaatt actgacgaag
    93601 tgcgcgttgt tttctggcaa atgcggcatg agcgtttgac ctttcctata atagggataa
    93661 ttttcaataa attgtgcttt gttcaccaag tctgatgagt gaagcacatc aagcaatttc
    93721 tcatttacaa tccgtctcaa ttacttgtta acacctctga caataccgat tctgcaagct
    93781 attcagtctt ttcattccat
//
LOCUS       DAZFZC010000005        89714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFZC010000005 DAZFZC010000000
VERSION     DAZFZC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.005"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            184..3201
                     /gene="ehaB"
                     /locus_tag="ACHKXP_000406"
     CDS             184..3201
                     /gene="ehaB"
                     /locus_tag="ACHKXP_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO7645480.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRANDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQVDLGSDIGTDTDGDGDVDNDTLDGKIDKNSPLDGIYDAYAMGS
                     DATATLIDIAANSLWPSSTVGVENSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDT
                     TKTSLIDTLSINSGSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVT
                     ISDEVSAGWAVGDAALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHA
                     GVFNIESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEA
                     GQNEVDNNGDRVAAATGNYKVRIDNATGAGSVADYNGNELIYVNDKNSNATFSAANKA
                     DLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHG
                     LADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNAKWIVGAAAGFAKG
                     DMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTN
                     SDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYEL
                     GVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFS
                     FTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            3289..3912
                     /gene="iprA"
                     /locus_tag="ACHKXP_000407"
     CDS             3289..3912
                     /gene="iprA"
                     /locus_tag="ACHKXP_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO7645481.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(3913..5070)
                     /gene="ampH"
                     /locus_tag="ACHKXP_000408"
     CDS             complement(3913..5070)
                     /gene="ampH"
                     /locus_tag="ACHKXP_000408"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO7645482.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNMLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYPRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            5422..6642
                     /gene="sbmA"
                     /locus_tag="ACHKXP_000409"
     CDS             5422..6642
                     /gene="sbmA"
                     /locus_tag="ACHKXP_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO7645483.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6655..7749
                     /gene="yaiW"
                     /locus_tag="ACHKXP_000410"
     CDS             6655..7749
                     /gene="yaiW"
                     /locus_tag="ACHKXP_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO7645484.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYSSRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(7808..8116)
                     /gene="yaiY"
                     /locus_tag="ACHKXP_000411"
     CDS             complement(7808..8116)
                     /gene="yaiY"
                     /locus_tag="ACHKXP_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO7645485.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            8376..8588
                     /gene="yaiZ"
                     /locus_tag="ACHKXP_000412"
     CDS             8376..8588
                     /gene="yaiZ"
                     /locus_tag="ACHKXP_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO7645486.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(8612..9706)
                     /gene="ddlA"
                     /locus_tag="ACHKXP_000413"
     CDS             complement(8612..9706)
                     /gene="ddlA"
                     /locus_tag="ACHKXP_000413"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7645487.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            9784..9987
                     /locus_tag="ACHKXP_000414"
     CDS             9784..9987
                     /locus_tag="ACHKXP_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645488.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            10169..10429
                     /gene="iraP"
                     /locus_tag="ACHKXP_000415"
     CDS             10169..10429
                     /gene="iraP"
                     /locus_tag="ACHKXP_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO7645489.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            10530..11945
                     /gene="phoA"
                     /locus_tag="ACHKXP_000416"
     CDS             10530..11945
                     /gene="phoA"
                     /locus_tag="ACHKXP_000416"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO7645490.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGRGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            12064..12384
                     /gene="psiF"
                     /locus_tag="ACHKXP_000417"
     CDS             12064..12384
                     /gene="psiF"
                     /locus_tag="ACHKXP_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO7645491.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            12486..13601
                     /gene="adrA"
                     /locus_tag="ACHKXP_000418"
                     /gene_synonym="dgcC"
     CDS             12486..13601
                     /gene="adrA"
                     /locus_tag="ACHKXP_000418"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO7645492.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(13618..14427)
                     /gene="proC"
                     /locus_tag="ACHKXP_000419"
     CDS             complement(13618..14427)
                     /gene="proC"
                     /locus_tag="ACHKXP_000419"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO7645493.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            14547..15011
                     /gene="yaiI"
                     /locus_tag="ACHKXP_000420"
     CDS             14547..15011
                     /gene="yaiI"
                     /locus_tag="ACHKXP_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO7645494.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            15188..15712
                     /gene="aroL"
                     /locus_tag="ACHKXP_000421"
     CDS             15188..15712
                     /gene="aroL"
                     /locus_tag="ACHKXP_000421"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO7645495.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            15762..15953
                     /gene="yaiA"
                     /locus_tag="ACHKXP_000422"
     CDS             15762..15953
                     /gene="yaiA"
                     /locus_tag="ACHKXP_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO7645496.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            16211..16888
                     /gene="aroM"
                     /locus_tag="ACHKXP_000423"
     CDS             16211..16888
                     /gene="aroM"
                     /locus_tag="ACHKXP_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO7645497.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLTKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            16960..17244
                     /gene="ppnP"
                     /locus_tag="ACHKXP_000424"
     CDS             16960..17244
                     /gene="ppnP"
                     /locus_tag="ACHKXP_000424"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO7645498.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            17392..17733
                     /locus_tag="ACHKXP_000425"
                     /pseudo
     CDS             17392..17733
                     /locus_tag="ACHKXP_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            17730..17813
                     /locus_tag="ACHKXP_000426"
     CDS             17730..17813
                     /locus_tag="ACHKXP_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645499.1"
                     /translation="MTQRPWSKLQRKTHNTAALKIIARRSE"
     gene            complement(17891..18802)
                     /gene="rdgC"
                     /locus_tag="ACHKXP_000427"
     CDS             complement(17891..18802)
                     /gene="rdgC"
                     /locus_tag="ACHKXP_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO7645500.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            18927..19835
                     /gene="mak"
                     /locus_tag="ACHKXP_000428"
     CDS             18927..19835
                     /gene="mak"
                     /locus_tag="ACHKXP_000428"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO7645501.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(19978..21162)
                     /gene="araJ"
                     /locus_tag="ACHKXP_000429"
     CDS             complement(19978..21162)
                     /gene="araJ"
                     /locus_tag="ACHKXP_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO7645502.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLMLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(21288..24434)
                     /gene="sbcC"
                     /locus_tag="ACHKXP_000430"
     CDS             complement(21288..24434)
                     /gene="sbcC"
                     /locus_tag="ACHKXP_000430"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO7645503.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNEPAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDAITKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPLDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQLLLTTLTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQD
                     ADNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQ
                     LTRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVS
                     HKTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKV
                     KKINGLGYSKLESTFAVK"
     gene            complement(24431..25633)
                     /gene="sbcD"
                     /locus_tag="ACHKXP_000431"
     CDS             complement(24431..25633)
                     /gene="sbcD"
                     /locus_tag="ACHKXP_000431"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO7645504.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            25823..26512
                     /gene="phoB"
                     /locus_tag="ACHKXP_000432"
     CDS             25823..26512
                     /gene="phoB"
                     /locus_tag="ACHKXP_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO7645505.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            26570..27865
                     /gene="phoR"
                     /locus_tag="ACHKXP_000433"
     CDS             26570..27865
                     /gene="phoR"
                     /locus_tag="ACHKXP_000433"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO7645506.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSIEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            28272..29591
                     /gene="brnQ"
                     /locus_tag="ACHKXP_000434"
     CDS             28272..29591
                     /gene="brnQ"
                     /locus_tag="ACHKXP_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO7645507.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            29667..31040
                     /gene="proY"
                     /locus_tag="ACHKXP_000435"
     CDS             29667..31040
                     /gene="proY"
                     /locus_tag="ACHKXP_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO7645508.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            31196..33013
                     /gene="malZ"
                     /locus_tag="ACHKXP_000436"
     CDS             31196..33013
                     /gene="malZ"
                     /locus_tag="ACHKXP_000436"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO7645509.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKTFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(33018..33599)
                     /gene="acpH"
                     /locus_tag="ACHKXP_000437"
     CDS             complement(33018..33599)
                     /gene="acpH"
                     /locus_tag="ACHKXP_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO7645510.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            33692..34762
                     /gene="queA"
                     /locus_tag="ACHKXP_000438"
     CDS             33692..34762
                     /gene="queA"
                     /locus_tag="ACHKXP_000438"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO7645511.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            34818..35945
                     /gene="tgt"
                     /locus_tag="ACHKXP_000439"
     CDS             34818..35945
                     /gene="tgt"
                     /locus_tag="ACHKXP_000439"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO7645512.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            35968..36300
                     /gene="yajC"
                     /locus_tag="ACHKXP_000440"
     CDS             35968..36300
                     /gene="yajC"
                     /locus_tag="ACHKXP_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO7645513.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            36328..38175
                     /gene="secD"
                     /locus_tag="ACHKXP_000441"
     CDS             36328..38175
                     /gene="secD"
                     /locus_tag="ACHKXP_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO7645514.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            38186..39157
                     /gene="secF"
                     /locus_tag="ACHKXP_000442"
     CDS             38186..39157
                     /gene="secF"
                     /locus_tag="ACHKXP_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO7645515.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            39286..39633
                     /gene="yajD"
                     /locus_tag="ACHKXP_000443"
     CDS             39286..39633
                     /gene="yajD"
                     /locus_tag="ACHKXP_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO7645516.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(39810..40694)
                     /gene="tsx"
                     /locus_tag="ACHKXP_000444"
     CDS             complement(39810..40694)
                     /gene="tsx"
                     /locus_tag="ACHKXP_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO7645517.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(40993..41532)
                     /gene="yajI"
                     /locus_tag="ACHKXP_000445"
     CDS             complement(40993..41532)
                     /gene="yajI"
                     /locus_tag="ACHKXP_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7645518.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMH"
     gene            41683..42132
                     /gene="nrdR"
                     /locus_tag="ACHKXP_000446"
     CDS             41683..42132
                     /gene="nrdR"
                     /locus_tag="ACHKXP_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO7645519.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            42136..43239
                     /gene="ribD"
                     /locus_tag="ACHKXP_000447"
     CDS             42136..43239
                     /gene="ribD"
                     /locus_tag="ACHKXP_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO7645520.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHH
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRSLLIPEHN
                     GHLDLVLLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            43328..43798
                     /gene="ribE"
                     /locus_tag="ACHKXP_000448"
     CDS             43328..43798
                     /gene="ribE"
                     /locus_tag="ACHKXP_000448"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO7645521.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            43818..44237
                     /gene="nusB"
                     /locus_tag="ACHKXP_000449"
     CDS             43818..44237
                     /gene="nusB"
                     /locus_tag="ACHKXP_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO7645522.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            44315..45292
                     /gene="thiL"
                     /locus_tag="ACHKXP_000450"
     CDS             44315..45292
                     /gene="thiL"
                     /locus_tag="ACHKXP_000450"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO7645523.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIGLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFVRDGEPVTLDWKGYDHFATP"
     gene            45270..45788
                     /gene="pgpA"
                     /locus_tag="ACHKXP_000451"
     CDS             45270..45788
                     /gene="pgpA"
                     /locus_tag="ACHKXP_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO7645524.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIIPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(45842..46816)
                     /gene="yajO"
                     /locus_tag="ACHKXP_000452"
     CDS             complement(45842..46816)
                     /gene="yajO"
                     /locus_tag="ACHKXP_000452"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO7645525.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTSIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(46996..48858)
                     /gene="dxs"
                     /locus_tag="ACHKXP_000453"
     CDS             complement(46996..48858)
                     /gene="dxs"
                     /locus_tag="ACHKXP_000453"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO7645526.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRSGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(48883..49782)
                     /gene="ispA"
                     /locus_tag="ACHKXP_000454"
     CDS             complement(48883..49782)
                     /gene="ispA"
                     /locus_tag="ACHKXP_000454"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO7645527.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(49782..50024)
                     /gene="xseB"
                     /locus_tag="ACHKXP_000455"
     CDS             complement(49782..50024)
                     /gene="xseB"
                     /locus_tag="ACHKXP_000455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO7645528.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            50230..51678
                     /gene="thiI"
                     /locus_tag="ACHKXP_000456"
     CDS             50230..51678
                     /gene="thiI"
                     /locus_tag="ACHKXP_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HFO7645529.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(51732..52322)
                     /gene="yajL"
                     /locus_tag="ACHKXP_000457"
     CDS             complement(51732..52322)
                     /gene="yajL"
                     /locus_tag="ACHKXP_000457"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO7645530.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWLDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(52285..53196)
                     /gene="panE"
                     /locus_tag="ACHKXP_000458"
     CDS             complement(52285..53196)
                     /gene="panE"
                     /locus_tag="ACHKXP_000458"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO7645531.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            53364..53855
                     /gene="yajQ"
                     /locus_tag="ACHKXP_000459"
     CDS             53364..53855
                     /gene="yajQ"
                     /locus_tag="ACHKXP_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO7645532.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(53983..55347)
                     /gene="yajR"
                     /locus_tag="ACHKXP_000460"
     CDS             complement(53983..55347)
                     /gene="yajR"
                     /locus_tag="ACHKXP_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7645533.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWINGMFDGQGVFLAGAMLAAVWLTVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(55496..56386)
                     /gene="cyoE"
                     /locus_tag="ACHKXP_000461"
     CDS             complement(55496..56386)
                     /gene="cyoE"
                     /locus_tag="ACHKXP_000461"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO7645534.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(56398..56727)
                     /gene="cyoD"
                     /locus_tag="ACHKXP_000462"
     CDS             complement(56398..56727)
                     /gene="cyoD"
                     /locus_tag="ACHKXP_000462"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO7645535.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(56727..57341)
                     /gene="cyoC"
                     /locus_tag="ACHKXP_000463"
     CDS             complement(56727..57341)
                     /gene="cyoC"
                     /locus_tag="ACHKXP_000463"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO7645536.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(57331..59322)
                     /gene="cyoB"
                     /locus_tag="ACHKXP_000464"
     CDS             complement(57331..59322)
                     /gene="cyoB"
                     /locus_tag="ACHKXP_000464"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO7645537.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(59344..60291)
                     /gene="cyoA"
                     /locus_tag="ACHKXP_000465"
     CDS             complement(59344..60291)
                     /gene="cyoA"
                     /locus_tag="ACHKXP_000465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO7645538.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(60751..62226)
                     /gene="ampG"
                     /locus_tag="ACHKXP_000466"
     CDS             complement(60751..62226)
                     /gene="ampG"
                     /locus_tag="ACHKXP_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO7645539.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(62270..62848)
                     /gene="yajG"
                     /locus_tag="ACHKXP_000467"
     CDS             complement(62270..62848)
                     /gene="yajG"
                     /locus_tag="ACHKXP_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7645540.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            63153..63470
                     /gene="bolA"
                     /locus_tag="ACHKXP_000468"
     CDS             63153..63470
                     /gene="bolA"
                     /locus_tag="ACHKXP_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO7645541.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            63814..65112
                     /gene="tig"
                     /locus_tag="ACHKXP_000469"
     CDS             63814..65112
                     /gene="tig"
                     /locus_tag="ACHKXP_000469"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO7645542.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            65358..65981
                     /gene="clpP"
                     /locus_tag="ACHKXP_000470"
     CDS             65358..65981
                     /gene="clpP"
                     /locus_tag="ACHKXP_000470"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO7645543.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            66107..67381
                     /gene="clpX"
                     /locus_tag="ACHKXP_000471"
     CDS             66107..67381
                     /gene="clpX"
                     /locus_tag="ACHKXP_000471"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO7645544.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            67569..69923
                     /gene="lon"
                     /locus_tag="ACHKXP_000472"
     CDS             67569..69923
                     /gene="lon"
                     /locus_tag="ACHKXP_000472"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO7645545.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            70114..70404
                     /gene="hupB"
                     /locus_tag="ACHKXP_000473"
     CDS             70114..70404
                     /gene="hupB"
                     /locus_tag="ACHKXP_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO7645546.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            70597..72468
                     /gene="ppiD"
                     /locus_tag="ACHKXP_000474"
     CDS             70597..72468
                     /gene="ppiD"
                     /locus_tag="ACHKXP_000474"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7645547.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEEQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            72619..72990
                     /gene="ybaV"
                     /locus_tag="ACHKXP_000475"
     CDS             72619..72990
                     /gene="ybaV"
                     /locus_tag="ACHKXP_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO7645548.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            73084..73482
                     /gene="fadM"
                     /locus_tag="ACHKXP_000476"
     CDS             73084..73482
                     /gene="fadM"
                     /locus_tag="ACHKXP_000476"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO7645549.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(73534..74229)
                     /gene="queC"
                     /locus_tag="ACHKXP_000477"
     CDS             complement(73534..74229)
                     /gene="queC"
                     /locus_tag="ACHKXP_000477"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO7645550.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(74294..75994)
                     /gene="ybaE"
                     /locus_tag="ACHKXP_000478"
     CDS             complement(74294..75994)
                     /gene="ybaE"
                     /locus_tag="ACHKXP_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO7645551.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            76094..76912
                     /gene="cof"
                     /locus_tag="ACHKXP_000479"
     CDS             76094..76912
                     /gene="cof"
                     /locus_tag="ACHKXP_000479"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO7645552.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            77065..77523
                     /gene="decR"
                     /locus_tag="ACHKXP_000480"
     CDS             77065..77523
                     /gene="decR"
                     /locus_tag="ACHKXP_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO7645553.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            77553..79325
                     /gene="mdlA"
                     /locus_tag="ACHKXP_000481"
     CDS             77553..79325
                     /gene="mdlA"
                     /locus_tag="ACHKXP_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7645554.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            79318..81099
                     /gene="mdlB"
                     /locus_tag="ACHKXP_000482"
     CDS             79318..81099
                     /gene="mdlB"
                     /locus_tag="ACHKXP_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7645555.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            81280..81618
                     /gene="glnK"
                     /locus_tag="ACHKXP_000483"
     CDS             81280..81618
                     /gene="glnK"
                     /locus_tag="ACHKXP_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO7645556.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            81648..82934
                     /gene="amtB"
                     /locus_tag="ACHKXP_000484"
     CDS             81648..82934
                     /gene="amtB"
                     /locus_tag="ACHKXP_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO7645557.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(82983..83843)
                     /gene="tesB"
                     /locus_tag="ACHKXP_000485"
     CDS             complement(82983..83843)
                     /gene="tesB"
                     /locus_tag="ACHKXP_000485"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO7645558.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            84061..84633
                     /gene="ybaY"
                     /locus_tag="ACHKXP_000486"
     CDS             84061..84633
                     /gene="ybaY"
                     /locus_tag="ACHKXP_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO7645559.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(84664..84975)
                     /locus_tag="ACHKXP_000487"
     CDS             complement(84664..84975)
                     /locus_tag="ACHKXP_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO7645560.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            85138..85234
                     /gene="ffs"
                     /locus_tag="ACHKXP_000488"
     ncRNA           85138..85234
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKXP_000488"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            85354..85707
                     /gene="ybaA"
                     /locus_tag="ACHKXP_000489"
     CDS             85354..85707
                     /gene="ybaA"
                     /locus_tag="ACHKXP_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO7645561.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(85749..87299)
                     /gene="pdeB"
                     /locus_tag="ACHKXP_000490"
     CDS             complement(85749..87299)
                     /gene="pdeB"
                     /locus_tag="ACHKXP_000490"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO7645562.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVAYIDNNV
                     PQCSSLEHESPPDTFPEPGKNSKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESPVLTSEKIPQLLRDMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(87463..87933)
                     /gene="ylaC"
                     /locus_tag="ACHKXP_000491"
     CDS             complement(87463..87933)
                     /gene="ylaC"
                     /locus_tag="ACHKXP_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO7645563.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(88049..88600)
                     /gene="maa"
                     /locus_tag="ACHKXP_000492"
     CDS             complement(88049..88600)
                     /gene="maa"
                     /locus_tag="ACHKXP_000492"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO7645564.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(88772..88990)
                     /gene="hha"
                     /locus_tag="ACHKXP_000493"
     CDS             complement(88772..88990)
                     /gene="hha"
                     /locus_tag="ACHKXP_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO7645565.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(89016..89390)
                     /gene="tomB"
                     /locus_tag="ACHKXP_000494"
     CDS             complement(89016..89390)
                     /gene="tomB"
                     /locus_tag="ACHKXP_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO7645566.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        21921 a        22158 c        23786 g        21849 t
ORIGIN      
        1 ttttatcaat ttgatgtata tttttctcca taaattatat atgtccacat tcggacttag
       61 gggaaagaat aattgaacca ttcgtctgta acgcagcata atcgttagcg cgaaacataa
      121 tatgttttct atgcattgat aattgatgga tcaacttatt acgtccctga ggagggatga
      181 caaatgcact cctggaaaaa gaaacttgta gtatcacaat tagcattggc ttgcactctg
      241 gctatcacct ctcaggctaa tgcagcgacc aacgatattt ctggtcaaac ttacaatact
      301 tttcatcact acaacgacgc cacctatgct gatgacgttt actatgatgg ttatgtaggt
      361 tggaacaact atgcagctga tagctattac aacggcgata tctacccggt cattaataac
      421 gctactgtta acggcgtgat ttctacctac tatctggacg acggtatttc taccaatacc
      481 aacgccaata gtctgacaat caaaaacagc actattcacg gtatgattta ttccgagtgc
      541 atgacaactg attgtgctga tcgtgcgaat gattattacc atgaccgtct ggcgctgact
      601 gttgataatt caacgatcga cgacaactac gaacactaca catataacgg cacttacaat
      661 aatgccgctg atactcatgt tgtaaacgta tataacattg gtactgctat tactctggat
      721 caggaagttg atctgtccat cactaataac tctcacgtag cgggtattac gctgactcag
      781 ggttatgagt gggaagatat tgacgacaac actgtcagca ctggtgtaaa cagcagcgaa
      841 gtgtttaata acaccatcac tgttaaagat tctaccgtga cctctggttc atggactgat
      901 gaaggtacta ctggttggtt tggtaataca ggtaatgcca gcgattacaa tggtgatggg
      961 tggaatgctg atgatgttgc attagctgtt attgcacatc catatgctga taacgccatg
     1021 caaaccactg caacattcga caattccaca ttgatgggcg atgtattctt ctcaagtaac
     1081 tttgatgaga acttcttccc acatggccgt gatagctatc gtgatgccga tggtgatgta
     1141 gatactaacg gttgggatgg tactgatcgc ctggatctga ccctgaacaa tggcagcaag
     1201 tgggttggtg cggcaatgtc tgcgcatcag gttgatctgg gttcagatat tgggacagat
     1261 accgatggcg acggtgatgt tgataacgat acccttgatg gtaagatcga taaaaacagc
     1321 ccgctggatg gcatttacga tgcttatgcg atgggttccg acgccactgc aactctgatc
     1381 gatatcgcag ctaacagcct gtggccttca tcaactgttg gtgtagaaaa cagcgactct
     1441 gaatacagcg aatttgatca catcatcggt aacgaagttt accagagcgg tctgttcaat
     1501 gtgaccctga acaccggttc acagtgggat accaccaaaa cttctctgat tgatactctg
     1561 agcatcaaca gtggttcaac tgttaatgtt gctgattcaa cgctgatttc tgactctatc
     1621 tctctgacag gtctttctgc gctgaacatc aacgaagatg gtcatgttgc aactgattca
     1681 ctgactgtcg acaacagtac cgtaaccatt tctgatgaag tttctgctgg ttgggctgta
     1741 ggtgatgctg ctctgtatgc caacaacatc aaagtgacta acgacggtat tctggatgtc
     1801 ggtaacactg cggcgaatgc tctgcaggtt gatactctga acctgaccag cactactgat
     1861 accagtggta acattcacgc tggtgtgttc aacatcgaaa gcaaccgctt cgtacttgat
     1921 gcagacctga ccaacgaccg taccaacgat actaccaagt caaactacgg ttatggctta
     1981 atcgcaatga actctgatgg tcacctgacc attaacggta acggcgataa cgacaacact
     2041 gcttctatcg aagctggtca gaacgaagtt gataacaacg gtgaccgtgt agctgcggca
     2101 accggtaact acaaagttcg tatcgacaac gcaactggcg caggttctgt tgctgactac
     2161 aacggtaacg agctgatcta cgtcaacgac aaaaacagca acgcgacctt ctctgctgct
     2221 aacaaagctg acctgggtgc atacacctat caggctgaac agcgcggtaa caccgttgtt
     2281 ctgcaacaga tggagctgac cgactacgct aacatggcgc tgagcatccc gtctgcgaac
     2341 accaatatct ggaacctgga acaagacacc gttggtactc gtctgaccaa ctctcgtcat
     2401 ggcctggctg ataacggcgg cgcatgggta agctacttcg gtggtaactt caacggcgac
     2461 aacggcacca tcaactatga tcaggatgtt aacggcatca tggtcggtgt tgataccaaa
     2521 gttgacggta acaacgctaa gtggatcgtc ggtgcggctg caggcttcgc taaaggtgac
     2581 atgaatgacc gttctggtca ggtagatcaa gacagccaga ctgcctacat ctactcttct
     2641 gctcacttcg cgaacaacgt ctttgttgat ggtagcttga gctactctca cttcaacaac
     2701 gacctgtctg caaccatgag caacggtact tacgttgacg gtagcaccaa ctccgacgct
     2761 tggggcttcg gcttgaaagc cggttacgac ttcaaactgg gtgatgctgg ttatgtgact
     2821 ccttacggca gcatttctgg tctgttccag tctggtgatg actaccagct gagcaacgac
     2881 atgaaagttg acggtcagtc ttacgacagc atgcgttatg aactgggtgt agatgcaggt
     2941 tataccttca cctacagcga agaccaggct ctgactccgt acttcaaact ggcttacgtc
     3001 tacgacgact ctaacaacga taacgatgtg aacggtgatt ccatcgataa cggtactgaa
     3061 gggtctgcgg tacgtgttgg tctgggtact cagttcagct tcaccaagaa cttcagcgcc
     3121 tataccgatg ctaactacct cggtggtggt gacgtagatc aagattggtc cgcgaacgtg
     3181 ggtgttaaat atacctggta atattcttca ctccgaagaa attctggtaa tttaatctaa
     3241 ataatgcccg tcaaggattt gacgggcatt actgcaaagg acgcgcaaat gttatctgta
     3301 gttaaacctc ttcaggaatt tggtaagctc gataaatgtt tgtccagata cggtacgcgc
     3361 ttcgagttta ataatgaaaa gcaagttata ttttccagtg atgtcaatag cgaagatact
     3421 ttcgttattt tagagggagt tatctctctg cgtagagaag aaaacgtact tatcggtatt
     3481 acccaggctc cttatattat ggggctggct gatggtttaa tgaaaaacga tataccatac
     3541 aaattaatat cagaaggaaa ttgtacggga tatcatctac cagccaaaca aaccattacg
     3601 cttattgaac aaaatcaact ctggcgagac gctttttact ggttagcctg gcaaaataga
     3661 attctggaat tacgcgacgt gcagctcatt gggcataatt cctacgaaca aatccgcgca
     3721 acattattat caatgattga ctggaatgaa gaattgcgat cacgtattgg tgtgatgaat
     3781 tatatccatc aacgtacacg catatcgcgt tctgtcgtcg cagaagttct cgctgctttg
     3841 cgtaaaggcg gctatatcga aatgaataaa ggcaaactgg tcgctatcaa ccgtttgcct
     3901 tcagagtatt aatcaggatg cggggataac caacggttta ttcccgctta gctcggtcac
     3961 cagatcattg atgccatcgc tcatattttt aaagcgcgtc aacggcgaac gagtgaccac
     4021 cacaaacgcg ccaatattct tctgcgggat catcgccata taggtaatga aaccgccacc
     4081 accgcctgtc ttctgaataa tccccggacg accctctttc ggggccatat atacccagcc
     4141 taaaccaagc gcatccgctt tgccggggac atccatgcca atcactttcg taaactgcgc
     4201 gcgcggataa atcaacgtct gcatgcgatc tgcctggtta ctgcgctgat aaaaatcaga
     4261 tgacaaatac tgctgcatcc agcgcatcat atcgccaggc gtggaataga cgccgccgct
     4321 gccaattgcc gccagcatgt tattgcacgg actggcaccg cgctcagcaa ccattaaacg
     4381 gcggcactga tccggtgagg gggtgtaggt ggtgtctttc atccccaatg gacgggtaat
     4441 ctgctcttca aacaactggg tataaggctt gccggacgca ttcgccagtg catctgccag
     4501 caggtcgaac gcaaggttag agtacgccgc ctgactgcct ggcgcggctt tcagcttcgc
     4561 cgtagaaagg tatttccagc gttgctcgcg cgttggccag acaaacaccg gacgatgtgc
     4621 cgcgccaccg ggctgttcac ggggcagggc gctggtatgg gttgccagat tcactagggt
     4681 aatgggcgtc ccattgtagg tcggcacgcg tgcgcctggc ggagcatatt tacttagcgg
     4741 atcgtttaac tttacggtcc cctggtcgag caatttcacc agcatttcac tggtcattag
     4801 tttggtgagg gaagcaatac gcacgaccga atccagctgc gggcgaacgt tattaccagg
     4861 tcgcgtttcg ccataactac gaaagacgcg ctggttaccg tcgataacaa ccagcgccat
     4921 ccccgtggca ccgctgccgt aaaaaatatg atcggcataa cgatcgacaa tatcagaggc
     4981 aaactccggt tcagtgatcg gctgcgccgc atggacggag gtcaatgacg ccgcacacag
     5041 cacggcagaa aaaagcagac tacgtttcaa cggtggtgtc cataaattga atgaaggtaa
     5101 ctgagcgtat ttatactact cattgatgga ttgcaaaggg cgaattagtg caataatagt
     5161 gttaaaaacg taacggcgcg taaacacgat ctttttcctg ctcaccaatg cctgcatttg
     5221 tgatcctgcg cgtgttaatc cgcttatgta aacaatcttt ggtttgtgaa atccgtcgaa
     5281 acaattctta tggtcaggct ggcgaactaa gcgccttgct atgggtcaca atgggcggtt
     5341 tcatttggct gacgctttgt agtggtcatg cggttaatac acagcagttg ttaaaacgat
     5401 aagaagttag caggagtgca tatgtttaag tcttttttcc caaagccggg agcgtttttt
     5461 ctctcggcct ttgtttgggc attgattgcc gttatcttct ggcaagccgg tgggggagac
     5521 tgggtggcgc gtatcactgg cgcttccggg cagatcccga ttagcgccgc gcgtttctgg
     5581 tcgttggatt tcctgatttt ttacgcttac tacattgttt gcgtaggact ttttgcattt
     5641 ttctggttta tctacagccc gcaccgttgg caatactggt caatactcgg tactgcactg
     5701 atcatcttcg tcacctggtt tttggtggaa gtcggggtcg ccgtcaacgc ctggtatgca
     5761 ccgttctatg atctgattca aaccgcgcta agttcgccgc ataaagtcac tatcgaacaa
     5821 ttttaccgcg aagtgggcgt ctttctgggg attgcgctga tcgctgtggt gatcagtgtg
     5881 ctgaacaact tctttgtcag tcactacgtg ttccgctggc gtacggcgat gaacgaatat
     5941 tacatggcga actggcaaca actgcgtcat atcgaagggg ccgcacagcg tgtgcaggaa
     6001 gacaccatgc gttttgcttc aacgctggag aatatgggcg tcagttttat caacgccatc
     6061 atgacgttga tcgccttcct gccggtgctg gtaacgctct ccacgcatgt gccggagctg
     6121 ccgattatcg ggcacattcc gtatggtctg gtgattgccg ctatcgtctg gtcgctgatg
     6181 gggaccggat tgctggcagt ggtagggatc aaactgccgg ggctggagtt taaaaaccag
     6241 cgagtagagg ccgcctaccg taaagagctg gtttatggtg aagacgatgc cacgcgtgcg
     6301 acgccgccta cggtacgcga gctgtttagc gccgtgcgga aaaactattt ccgcctctat
     6361 tttcactata tgtatttcaa catcgcccgc attctctatt tgcaggtcga taacgttttc
     6421 ggtttgttct tgctgtttcc gtcaattgtt gccggtacga ttacgctcgg cctgatgacg
     6481 cagattacca acgtttttgg tcaggttcgc ggtgctttcc agtacctgat taactcatgg
     6541 accacactgg ttgagttgat gtctatctac aaacgtctgc gcagctttga acatgaactg
     6601 gatggtgaca aaattcagga agtaacccat accttgagct aaaaaggaga agtaatgtcg
     6661 cgcgtcaatc ctctatcttc gctgtcattg ttggcggttc tggttttagc ggggtgtagt
     6721 agtcaggctc cgcaaccgtt gaaaaaggga gaaaaagcga tagatgtggc gagcgtcgtg
     6781 cggcagaaga tgcctgcgag cgtgaaagac cgcgatgcct gggcgaaaga tctcgccacc
     6841 acctttgaaa gtcaggggct ggcaccgacg ctggaaaacg tctgctcggt gctggcggtg
     6901 gcgcagcagg agtcaaatta tcaggcagat ccggccgttc cgggcttaag caagatcgcc
     6961 tggcaagaga ttgaccgtcg tgctgaacgg atgcacattc ctgcttttct ggttcatacc
     7021 gcgctaaaaa tcaaatcgcc aaacggcaaa agttatagcg aacggttaga ttcggtgcgt
     7081 acagaaaagc aattgagcgc gatttttgac gatctgatca gcatggtacc aatggggcag
     7141 acgctgtttg gttcgcttaa tccggtgcgc accggtgggc cgatgcaggt cagcattgct
     7201 tttgccgaac agcataccaa agggtatccg tggaaaatgg acggtacagt tcgtcaggaa
     7261 gtcttcagtc gccgcggcgg gttgtggttt ggtacttacc atttactgaa ttatcccgcc
     7321 agctatagcg caccgatata ccgctttgct gattttaacg ctggctggta ctccagccgt
     7381 aatgccgcgt ttcagaacgc agtcagtaag gccagcggcg tgaagctggc actggacggt
     7441 gatttaattc gttacgacag taaagaaccc gggaaaaccg aactggcaac gcgcaaactg
     7501 gcgggaaaac tggggatgag cgacagcgaa attcgccgtc agttagagaa aggtgacagc
     7561 ttctcttttg aggagacggc gctgtacaag aaagtttatc aacttgccga agcgaaaacg
     7621 ggtaaatcac taccccgcga aatgttgcct ggcattcaac tggaaagccc gaaaatcacc
     7681 cgcaacctga ctacggcctg gtttgcgaag cgcgtagacg aacggcgggc gcgttgtatg
     7741 aaacagtgat caaacaggaa tgtcatgcca gataaggcgt tttaggccgt atctggcaac
     7801 gtaaatctta ctggcggcga tgtcgccagt gcagccatag cgccaccact gcaaaaatga
     7861 ggcagcctac taaaaacggg atcagcccga aaatggtgcc gacacctaaa ccaatttcca
     7921 cccgtgggcg acctgtttcc tggacctgca ttaaacgctc atagacgccg ggcgcatgca
     7981 ctaacaggtt cagcaattgc gccccagccc aaaagcaaaa cagcacaaac aacgcatagg
     8041 caatgttgcc aggcgttgaa gaggcattgc gatattttcc gctaaacagc gattttgaca
     8101 gggtgaaatc agccataaaa acctccggaa gtcatctgct tctcttctgg tgtgatacca
     8161 gcggcttatg gcaagtctga caggtcattt agattgtcaa tatcagaatc atggtaaatt
     8221 gatgttggga atattcccga agcgtgaatc ttcgttgtga gtcacaaaat ggcaacctta
     8281 agttaaattt attaacaatt acgaaaatgt tgtctacacc acagacggtt gttacagaat
     8341 atgcaaggat gttgtttttc gttaacggag ctgccatgaa tctgcctgta aaaatccgcc
     8401 gtgactggca ctactatgcg ttcgccattg gccttatatt cattcttaat ggcgtggtgg
     8461 ggttactggg atttgaagca aaaggttggc agacctatgc cgtcggtctg gtgacgtggg
     8521 tgattagttt ctggctggcg gggttgatta ttcgtcgtcg cgatgaagaa actgaaaacg
     8581 cccaataaga atattatcgg gcgttaaaat attacattgt ggttttcaat gcgttatccg
     8641 cagcgtgacg ctccagcgcc agctcaatca aacgtgtgat cagatcggtg taacccagac
     8701 cgctggcttg ccacagcttc ggatacatac tgatgttggt gaagccaggc agtgtgttga
     8761 tctcgttgat caccacttcg ttctctgggg ttaaaaacac gtctacacgc gccatgcctg
     8821 cgcatcccaa cgtctgataa gcctgaacgg caatcgcccg gatcttatcg ttgatctctg
     8881 gtgcaatggc tgccggaact accactttcg cgccatcttc gtcaatgtac ttggtgtcgt
     8941 aggcatagaa gtcgctggtg agtacgatct cgccacaggt gctggcttgc gggttgtcgt
     9001 tgcccagaac tgcgcattcg atctcacgac ctttgatccc ttgctcaacg atcactttat
     9061 gatcgaattc gaacgccaga tcgacggcaa ttgcgtactg ttcttcactg gttactttgc
     9121 tgacaccaac agaagagccc tgattagccg gttttacaaa cagtggtaac cccagtttag
     9181 actccacttc ggcaaaactg atgttgtgac gattagcgcg cgtcagggta ataaatggcg
     9241 caatgttcag cccggcatcg cgtagcagac gtttggtgac atctttatcc atacaggctg
     9301 ctgaagccag aacatcagaa cctacaaacg gtaaattggc gacccgcagc attccctgca
     9361 aggaaccatc ttcgcccagc gtaccgtgga caatcgggaa aatgacatcc accgtcggca
     9421 acggctgacc gttttgcgcg tcgataagct gatgctcatg tttacctggc acctgcgcaa
     9481 ggctggtcgc cgaagggcgc aacgcaatat gggcaggatc gtctgcattt agcagataat
     9541 tgctggcatc gctgacgtgc cattgccctt gtttatcaat gcccagcagc acaacgtcga
     9601 agcgactttt atcaatggca tcgacaatgt tttttgccga ttgcagagac acttcatgtt
     9661 ccgctgattt accaccaaaa acgattccta cccgcagttt ttccatctta aaaacctatc
     9721 ccgtctaaca caaagtgcat acattaccac gacaaaacgg gggattcgcg gccttctgaa
     9781 agattgttgc aatcttctgc tgacaaagcg tgcaacgtac tggtgaagaa agtgcgttat
     9841 ctcaaagatg tgcgcaagat cacaaaaatg atgaacggga agctaattta ttcctggctt
     9901 aaatggccat gcggtgagtt tttttctctt aattataagt taacgaagag aatatatttc
     9961 ataactttta tttataataa aggttgataa ttaaaagcct atattttgtg tgggtagtta
    10021 tttaaataag agaaacgttt cgctggtaat caaacaaaaa atatttgcgc aaagtatttc
    10081 ctttgtcata aaaataatac ttccagacac tatgaagttg tgaaacataa tgttaacttc
    10141 tccatacttt ggataaggaa atacagacat gaaaaatctc attgctgagt tgttatttaa
    10201 gcttgcccaa aaagaagaag agtcgaaaga actgtgtgcg caggtagaag ctttggagat
    10261 tatcgtcact gcaatgcttc gcaatatggc gcaaaatgac caacagcggt tgattgatca
    10321 ggtagagggg gcgctgtacg aggtaaagcc cgatgccagc attcctgacg acgatacgga
    10381 gctgctgcgc gattacgtaa agaagttatt gaagcatcct cgtcagtaaa aagttaatct
    10441 tttcaacagc tgtcataaag ttgtcacggc cgagacttat agtcgctttg tttttatttt
    10501 ttaatgtatt tgtacatgga gaaaataaag tgaaacaaag cactattgca ctggcactct
    10561 taccgttact gtttacccct gtgacaaaag cccggacacc agaaatgcct gttctggaaa
    10621 accgggctgc tcagggcgat attactgcac ccggcggtgc tcgccgctta acgggtgatc
    10681 agaccgccgc tctgcgtgat tctcttagcg ataaacctgc aaaaaatatt attttgctga
    10741 ttggcgatgg gatgggggat tcggaaatta ctgccgcacg taattatgcc gaaggtgcgg
    10801 gcggcttttt taaaggtatc gatgccttac cgcttaccgg gcaatacact cactatgcgc
    10861 tgaataaaaa aaccggcaaa ccggactacg tcaccgactc ggctgcatca gcaaccgcct
    10921 ggtcaaccgg tgtcaaaacc tataacggcg cgctgggcgt cgatattcac gaaaaagatc
    10981 acccaacgat tctggaaatg gcaaaagccg caggtctggc gaccggtaac gtttctaccg
    11041 cagagttgca ggatgccacg cccgctgcgc tggtggcaca tgtgacctcg cgcaaatgct
    11101 acggtccgag cgcgaccagt gaaaaatgtc cgggtaacgc gctagaaaaa ggcgggagag
    11161 gatcgattac cgaacagctg cttaacgctc gtgccgatgt tacgcttggc ggcggcgcaa
    11221 aaacctttgc tgaaacggca accgccggtg aatggcaggg aaaaacgctg cgtgaacagg
    11281 cacaggcgcg tggttatcag ttggtgagcg atgctgcctc actgaatgcg gtgacggaag
    11341 cgaaccagca aaaacccctg ctaggactgt ttgctgacgg caatatgcca gtgcgctggc
    11401 aaggaccgaa agcaacgtac cacggcaata tcgacaagcc cgcagttacc tgtacgccta
    11461 atccgcaacg taatgacagc gtaccgaccc tggcgcagat gactgataaa gccattgaat
    11521 tgttgagtaa aaatgagaaa ggctttttcc tgcaagttga aggtgcatca atcgataaac
    11581 aggatcacgc tgcgaatcct tgtgggcaaa ttggcgagac ggtcgatctc gacgaagccg
    11641 tacaacgggc gctggaattc gctaaaaagg atggcaacac gctggtcata gtcaccgctg
    11701 atcacgccca cgccagccag attgttgcgc cggacaccaa agcgccgggc ctcacccagg
    11761 cgctaaatac caaagatggc gcagtgatgg tgatgagtta cgggaactcc gaagaggatt
    11821 cacaagaaca taccggtagt cagctgcgta ttgcggcgta tggcccacat gccgccaatg
    11881 tcgttggact gaccgaccag accgatctct tctacaccat gaaagccgcc ctggggctga
    11941 aataaaaccg cgcccggcgg tgaattttcg ctgtcgggtg gtttttttgc tgttagcagc
    12001 cagacttaat ggcagatcac gggcgcaaac gctcatggtt aaaacatgaa gagggatggt
    12061 gctatgaaaa taacattact ggttaccttg cttttcggac tggttttttt aaccaccgtc
    12121 ggcgctgccg agagaacctt aaccccgcaa caacagcgta tgacctcctg taatcagcag
    12181 gcgacggcgc aggcgttgaa aggggatgct cgtaagacct acatgagtga ttgcctgaag
    12241 aacagcaagt ctgcgcctgg tgaaaaaagt ttgacgccac agcagcaaaa gatgcgcgaa
    12301 tgtaataatc aagcaacgca acaatctctg aaaggtgatg atcgtaataa gtttatgagt
    12361 gcctgcctca agaaagccgc ctgatacctg atagtgctaa cgggtgagct acgaaagtgg
    12421 ctcacccgaa atttcatact tctgccttta gccccgtctc tataatttgg gaaaattgtt
    12481 tctgaatgtt cccaaaaata atgaatgatg aaaacttttt caaaaaagcg gcggcgcatg
    12541 gggaggaacc tcctttaact cctcaaaacg aacatcagcg gtccgggctg cgcttcgccc
    12601 gtcgcgtcag actaccccgt gcggttggcc tggctggcat gttcttaccg attgcttcaa
    12661 cgctggtttc gcacccgccg ccgggctggt ggtggctggt gttggtcggc tgggcgttcg
    12721 tctggccgca tttagcctgg cagatagcga gcagggccgt cgatccgctt agccgggaaa
    12781 tttacaactt aaaaaccgat gcagtattag cgggaatgtg ggtaggcgta atgggcgtaa
    12841 acgtgctgcc ttccaccgcg atgttgatga ttatgtgtct gaatttgatg ggggcaggcg
    12901 gcccccgtct gtttgtcgcg ggtctggtgt tgatggtggt ttcctgcctt gtcaccctcg
    12961 agctgacggg cattaccgtg tcgttcaata gtgcgccgct ggaatggtgg ctctcccttc
    13021 ccattattgt catttatcct ctgctgtttg gctgggtcag ctaccagacg gcaaccaaac
    13081 tggcggaaca taaacgcagg ttgcaggtca tgagtacccg cgacggcatg acgggcgtgt
    13141 ataaccgacg tcattgggaa actatgttac gcaatgaatt tgataactgt cggcggcata
    13201 atcgcgatgc aacgttactg attatcgata tcgaccattt caagagcatc aacgatacct
    13261 ggggccatga tgtgggcgat gaagcgatag tggcgcttac ccgacagtta caaattaccc
    13321 tgcgcggtag cgatgtgatt ggtcggtttg gcggcgatga gtttgcagta atcatgtccg
    13381 gtacgccagc tgagagcgct attaccgcca tgttacgagt gcatgaaggg ctaaatacat
    13441 tacgtttgcc gaatacgcca caggtaactt tacggattag tgtgggggtt gcgccgctga
    13501 acccacaaat gagtcactat cgtgagtggt tgaaatcggc agatttggcg ctttacaaag
    13561 caaagaaagc cggacgtaac cgcaccgaag tggcggcctg acgtccggcg aaagtcatca
    13621 ggatttgctg agtttttctg atttttccat acacttcgtc atcgcttcga tcactgcggc
    13681 acggaagcct ttctcttcca gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca
    13741 gaccatatct ttcagtgccc ccggatgttc tcccgtttcc agcaccattt ttgcggaacc
    13801 cattactgcc tgagcggcaa atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc
    13861 ggcgtcggcc atcgcttcga taaacataaa tacgtaggct ggcgaagaac cgctcacacc
    13921 gaccaccggg tggatcatcg gctcagcaat cacttccgct tcgccaaagc agcggaaaat
    13981 attcagcaca tcagcggtat cttctggggt taccagcgcg tttggcgtta cggaggtcat
    14041 cccggcatta accagcgcgg gagtgttcgg catggcgcgg ataattttcc ggtcatggcc
    14101 cagcgcgcgg gcaagttggt cgagcgtgac acctgcagca atagaaacga ccagagagtc
    14161 tttattcagg ctggaggtga tttcgctaag cactttaatc atgatgccag gtttaacggc
    14221 agcaaaaatg atgtcggcga tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc
    14281 gaactggtca tgcagggcgg cgactttatc cggggagggg gtatataccc agatttgccc
    14341 tggaagcacc tgaccgctgg caatcagacc gccgagaatg gcttttccca tattgccgca
    14401 gccaataaaa ccgattttct tttccattgc ctcactcctg ccgtgaaatt tattgttttg
    14461 ataatcgctg gcagaagcat aaacagaact atgccggaag gcaaaagcgc gacacaatag
    14521 aggattaccc aacaaaggat gactttatga caatttgggt ggatgccgac gcgtgtccca
    14581 atgtaattaa agagattttg tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg
    14641 tagcaaacca gagtttacgc gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg
    14701 caggtttcga cgttgccgat aacgaaattg tccggcagtg tgaagcgggc gatttggtga
    14761 tcaccgcaga tatacctttg gctgctgaag ccatcgagaa aggcgctgcg gcgattaatc
    14821 cgcgcggcga acgttacacg ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta
    14881 tggatacctt acgtgccagt gggatccaga ccggcggacc agatagtctt tcacaacgtg
    14941 accgccaggc ctttgctgcg gagctggaga aatggtggct ggaagtgcaa cgtagtcgcg
    15001 gccaaatgta atttattatt tacacttcat tcttgaatat ttattggtat agtaaggggt
    15061 gtattgagat tttcacttta agtggaattt ttactttaca atcgaaattg tactagtttg
    15121 atggtatgat cgctattctc atgacaccgg ctttcgccgc attgcgacct attggggaaa
    15181 acccacgatg acacaacctc tttttctgat cgggcctcgg ggctgtggta aaacaacggt
    15241 cggaatggcc cttgccgatt cgcttaaccg tcggtttgtc gataccgatc agtggttgca
    15301 atcacagctc aatatgacgg tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg
    15361 cgccagagaa acggcggcgc tggaagcggt aactgcgcca tccaccgtta tcgctacagg
    15421 cggcggcatt attctgacgg aatttaatcg tcacttcatg caaaataacg ggatcgtggt
    15481 ttatttgtgt gcgccagtat cagtcctggt taaccgactg caagctgcac cggaagaaga
    15541 tttacggcca accttaacgg gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga
    15601 acgcgatgcg ctatatcgcg aagttgcgca tattatcatc gacgcaacaa acgaacccag
    15661 ccaggtgatt tctgaaattc gtagcgccct ggcacagacg atcaattgtt gattttcgag
    15721 cgcctatact taacgttcat cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt
    15781 atcctcgtga agcatatata gtgacgattg aaaaaggaaa gccaggacag acggtaacct
    15841 ggtaccaact cagagccgat catcctaaac cagactcgtt gatcagtgaa catccgacgg
    15901 ctcaggaagc gatggatgcg aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc
    15961 agactgctcg gaagggattc tgagtgccac tacaagggat ctgcgtcaca tttttcataa
    16021 ttcatgtttt tctaataatt agaatattaa acaataacaa tccattactg gaatcatttg
    16081 gaatctttac attatgccgt gcacgtctgc tgctacgctt tttgtcattt gtagcacaag
    16141 taagtgtcag cagtggtgct tcacacttgc ccggtaatta acgacgaaag aaaagtaagg
    16201 tggatgaaca atgagtgcgt cgttggcgat cctgaccatc ggcattgtac ctatgcagga
    16261 agttttgccg ctcctgacgg aatacattga cgaagataat atttcccatc atagcctgct
    16321 ggggaagtta agtcgtgaag aagtgatggc ggagtacgcg ccagaagcag gcgaagacac
    16381 cattctcaca ttattaaatg acaaccatct ggcccatgtt tcgcgtcgca aagtggagcg
    16441 agacctgcaa ggtgtggttg aagtgctcga taatcagggt tatgacgtca ttttattaat
    16501 gagtacagca aacattagta gtatgactgc gcgtaatacg atctttcttg agccgtcgcg
    16561 aatattgcct ccactggttt cctctattgt tgaagatcat caggtggggg ttatcgttcc
    16621 ggttgaggag atgctgaccg ttcaggcgca aaaatggcaa attttgcaga aatcgccggt
    16681 attttcattg ggtaacccca ttcatgattc agaacaaaaa atcattgatg ccgggaaaga
    16741 attactgaca aaaggggctg atgtcatcat gctggattgt ttaggatttc accaacgtca
    16801 tcgcgattta ctgcaaaaac agctcgatgt tcctgtcttg ctgtctaacg tattgattgc
    16861 acggctggct gcggaattac tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta
    16921 tagtgaagta gatgttcaac taccagacag ggccagttta tgcttcaaag taatgagtac
    16981 ttttccggca aagtgaaatc aatcggcttt tccagcagca gcactggtcg cgccagcgtg
    17041 ggtgttatgg ttgaaggcga atacaccttc agcaccgctg agccggaaga gatgacggta
    17101 atcagtggcg cgctgaatgt gttactgcct gacgcgaccg actggcaggt gtatgaagcc
    17161 ggttcggtgt ttaatgttcc cggtcacagt gagtttcatc tgcaagttgc cgaacccacc
    17221 tcttatctgt gccgctatct gtaattcctc gccttcccct tgaacgggag ggcatttttc
    17281 tgaaatatcc tctctttaac ccataataat atttcctttg ctgcgatttt ttcaatttcc
    17341 gatatattca taattcatca aggttgatat aaatatcagt gaagatctcc agatattgtt
    17401 gcggaactgg ctacgataaa agataaatca gatgatgaat ggtggcgtgc attgctgcaa
    17461 agtcgcaatg atcatttgcg ccaaacggca ttacgcaacg cacatacccc ggcgtcgttg
    17521 ttaacgacat tgaccgagtc tcggcatcgg tcgctagcta tgaataatcc acagctcgct
    17581 gccgatgtga aaacgacgtg gttaaaagag gatccatcat tactcttatt tgtcgaacaa
    17641 cccgatcttt cgctgttacg ggatttagtg aaaaccgggg caatgcggaa aattcgcagt
    17701 gaagcgcgtc actggcttga ggaaaaacaa tgacacaacg tccatggagc aagttacagc
    17761 gtaaaacgca taatactgct gcgctaaaaa ttattgcccg ccgtagcgaa taattatatg
    17821 cctggtgtgg cttcgtacgc cggataagac gcagcaggcg tcgcatccgg cattaaagga
    17881 aaatcagcaa ttaacgttgt gcttcgccac ctaatccttc aatcaggttt tgaattaacg
    17941 ctgccagttc accagtcatc aggatgaaat cggcatcaaa acgctgggcg aaatcttcac
    18001 ggtcgatatc ttcgttttga tcgcgcagct cgtcgcagaa cttcagacgc ttgagcgaac
    18061 catcgtcgca catcacaaac tgaatgcgct gctgccagtc gagcgccagt ttagtcacca
    18121 cttttccggc ttcaatgtga ttggtgatct cttcgctggt cagatcttgt ttcttcgcgc
    18181 ggatcactcc gccatcttcc agcaacgatt tcagctcggc ttcatcaagc aactggaagc
    18241 cctgtgccgc actaccggaa cgtacccatt cggtcagcgt cagttcaatc gggttttcca
    18301 tgctcaatgg tacaaccggt aacgacccca ggcttttacg cagcagtgcc agcgtatctt
    18361 ccgctttttt ggcactggcg cagtccacca taatcaaacc gttaaccgtg tcgatccaca
    18421 tcattgtctg gctaaaacgg ctgaaagcac gcggcaacag agagtgcagc acttcgtctt
    18481 tcagcgaatc tttttcggtt ttcttcagct tacgcgcctg ttccgcttcc agtttggcga
    18541 ttttcgcttc cagcgcctgt ttaatcaccg gagacgggag gattttttct tctttgcgcg
    18601 cgcagataac aatttgacca ttggcaacgt gcgttaacgc atcgctgtgc gatcccatcg
    18661 gaggaaccca gcccatcttc gccatgtcct ggctgccgca tggggtaaat gccatcgagg
    18721 ctagctgttt ttccatctct tctgcacgca gcgaaatctc gcggctaaga cggtaaacca
    18781 ttaaattttt gaaccacagc atgataattt ccacggcctt gtcgttaaat ttagcgggca
    18841 tgataacgaa ttgtcggcgg ccttgcattg ccaatccggt tgtccgtctc tacgctattg
    18901 atattgaaaa aaataaggag agtaccgtgc gtataggtat cgatttaggc ggcaccaaaa
    18961 ctgaagtgat tgcactgggc gatgcagggg agcagttgta ccgccatcgt ctgcccacgc
    19021 cgcgtgatga ttaccggcaa actattgaaa cgatcgccac gttggttgat atggcggagc
    19081 aggcgacagg gcagcgcgga acggtaggta tgggcattcc aggttcgatt tcgccctaca
    19141 ccggtgtggt gaagaatgcc aattcaacct ggctcaacgg tcagccattc gataaagact
    19201 taagcgcgcg gttgcagcgg gaagtgcggc tggcaaatga cgccaactgt ctggcggttt
    19261 cagaagcggt agatggcgcg gcagcgggag cgcagacggt atttgccgtg attatcggca
    19321 cgggatgcgg cgcgggcgtg gcattcaatg ggcgggcgca tatcggcggc aatggcacgg
    19381 caggtgagtg gggacacaat ccgctaccgt ggatggacga agacgaactg cgttatcgcg
    19441 aggaagtccc ttgttattgc ggtaaacaag gttgtattga aacctttatt tcgggcactg
    19501 gattcgcgac ggattatcgt cgtttgagcg gacatgcgct gaaaggcagt gaaattatca
    19561 gcctggttga agaaagcgat ccggtagcgg aactggcatt gcgtcgctac gagctgcggc
    19621 tggcaaaatc gctggcacat gtcgtgaata ttctcgatcc ggatgtgatt gtcctggggg
    19681 gcgggatgag caatgtagac cgtttatatc aaacggttcc gcagttgatt aaacaatttg
    19741 tcttcggcgg cgaatgtgaa acgccggtgc gtaaggcgaa gcacggtgat tccagcggcg
    19801 tacgcggcgc tgcgtggtta tggccacaag agtaaaaaac gtaggcaatt ggcgcatcat
    19861 gcctgatgcg acgcttgccg cgtcttatca ggcctacaaa atgtgccaga accgcgtagg
    19921 gcggataagg cgttcacgcc gcatccggca ataagcaacg cgcaccgtga cagtaatcta
    19981 ccccagtggt ttcgccagca ccggactatc cgccgcttgc tggcgcttat agcgaccata
    20041 cagcagcaac gacgacatcg cagcaaacga aagcagggcg gcaggcagcg ccacgtaatt
    20101 atatgccagc cccagcatca gcatcatacc tccgcaatat gcgccgacgg cgctaccgag
    20161 gttaaacgct atttgcccac ctgcggcacc taataactct ccgcctttgg cgttttgtag
    20221 taacaatatt tgcagaggtg ctgaaagggc aaataatccc gcgcaacaaa taaaagcaaa
    20281 aataagcgac gttgttttca tgccgccgca gaaaaaaagc atcagcagtg ccagtacaat
    20341 gataaagtca gttactgctg caatgcgcag tggtgaataa cgtcctgaaa tcctgccact
    20401 tagcacattc cccagcacca tccctagccc aactaacatc ataataaagg tcatcgccgt
    20461 ttccgaaaaa ccggaaataa acatcatgta tggctttacg tagctgaacc aggcaaacac
    20521 acctgcgttg ccaaacatgg tggcggcgaa aattaaccac ggggccgggc tgcgcaaaaa
    20581 gtgaaattgt tcgcgcagct ttcctttcgc ctcgtcgcga atatctggca cccaaaaata
    20641 gaccgatgcc atcaccgcaa tattaaaaac agcgatcaat aaaaaggtgt aacgccagct
    20701 aaattcctga cttaaatacg ttcccagcgg aatgcccagc aaattggcga ctgtcatccc
    20761 ggaaaccatc cccgccacgg cggcggtgac ttttccgggt ttgataattt ttgataacac
    20821 gatcgctccg acgccaaaaa atgcgccatg cggaaagccg gataccagcc gaccaatggc
    20881 gagcatcagg taagacgaag agagcgtgaa catggcgttg ccaatgacgc acaacgccac
    20941 cagaaacaac aagatatgtt tgagtgagta gcggctggaa aagagtgcga tgattggcgc
    21001 accgaccacc acccccagtg cataatacga gatcatatgc ccggcagcag gaatcgaaat
    21061 tcctacgtta tgcgccagct ccgtgagcac gcccataatg ccaaattcgg ccatccccaa
    21121 accaaacgtg cccagagcca aagataaaat gacttttttc ataccaccat cacaaccacc
    21181 agaacggcta ccaatcaccg tagctaataa agtgatagct tgcaggataa cgaccagcaa
    21241 ggccaagaga taattcgccc ctctgtattc attatcctgc taaatagtta tttcactgca
    21301 aacgtacttt ccagtttgct gtagcccagg ccgttgatct ttttcacttt gatctgcacc
    21361 ggaatacgct ctttcatcgc ttctacgtgg ctaatcacac cgatggtttt gccactggcg
    21421 ttcagggcat ccagcgcatc aagggcggta tccagcgttt cgctatccag cgtgccaaaa
    21481 ccttcatcaa ggaacagcga gtcaatacgt gttttatggc tgaccagatc cgaaagcgcc
    21541 agcgccagcg ccagactaac gaggaaactt tcgccgccgg aaagggtacg ggtatcgcgt
    21601 accgcatctg cctgccaggt atcaacaacc tcgacttcca gcgcctcgct ggctttgcgc
    21661 tgtaacagat agcgcccgtg cagccgggta agttgctgat tagcgagatg gactaaatta
    21721 tccagcgtca gcccctgggc aaacttgcgg aatttatcgc cctctttgga acctattagc
    21781 gaattcagat atccccagtc ctcaacctgc tgcgtcattt gagcaatttg ctgcattaag
    21841 gtttgttgtt gctgacggtt atctgcatcc tgcttcagct gctggcgaat ctcgccttga
    21901 ctcgtggtgt tttcacgcaa cttttggtga gtttgcgcta actcttgctg aatctgctcc
    21961 accgtcacag tgagagccaa cccgtcgtca ggtcggtgtt gttgatgctg tgccagcgtt
    22021 tctgctgtct gagtgaccag agtttgcgcc tgacggcgct ggttttccag attctgcttg
    22081 agctgttcca gctgcgttag tgtttgttca tccattagcg ccgcaaggaa cgcctgctga
    22141 tcgtcaaaga cgctggcctg tagcgcggtg tcaaactgcg cctgggcttt ttgcagactt
    22201 tgcgccgcca gaacatcctg ttgctgtaac gtctgctgct ggctgtgtaa tgcgagacat
    22261 tgttcatgta cctgccgcca gttttccaat accacagttt cttcgcagtg cgggagttca
    22321 tcactttgcg gcaacgtttc cagaatcggc gtcagctgct gaatacggtt ttgcagcgcg
    22381 gttaattcgt tctggcgttg ctgccagctc tgcgcttctt gctgacgtgt cgccaaccag
    22441 ctctcttctt catcttcctg tggcaatgtc agtgcataac ccgtcaatgt cgttaaaagt
    22501 agttgctggc gttgttcaat ttgctgttga tactggataa tttgctgatt atgcgcggca
    22561 atctgccctt gtaattcatg ccgttggctg agtaaccgca gctggcgttc gtgctcatct
    22621 tgtgcatcca gccacggttg aatatcgtcc agtggctgca aggtgatatt gaggctggcc
    22681 gtgacggctt gccattgttg agtaagtgct tgctcatctt gtcggaggct ttgcgcttcg
    22741 ttttcatcac gctgaagctg ctttgttatg gcgtccagtt gcccacgtag cgtcgcacct
    22801 tcttcaccga gctttttaac ttcgttttcc agcgccagta atcgagactg attaacgcca
    22861 ggctccagcg cctgatacgc ctcgaccgcc gggtggctgg tggaaccaca aagtgggcaa
    22921 ggctgacccg cctgtaactg tgcacgttga gcttccagcg ttttgatgcg cgcttcctgc
    22981 tcgcaaatgg ttttcacatc ggcaagttgc tgcgtctttt ctttataacg ctggcgcatt
    23041 tcgttaagtg cggcgttacg ttgcgtctgt tcttgcgtga cattctggat agcgacctgt
    23101 aactgcgcca gacgtttttg ttggggaaca atctgtccat gcagcgcgac caggtgctga
    23161 cgcagtgggc gttgctcagc atgttgcgcc agggcggtag caacttcatc ggcggttaac
    23221 gtcaacgtga tcgccgcaag cgcattaagt ttttgctcag catgggttaa ctgttgctgc
    23281 cattgccgca gatgctcgcg atcgctggtt tgttgggaga actgcgcacg ccaacccgcc
    23341 ggttcgttgt tccactgacg gaagcggtcg tgttcctgta accaggtatt caggctttgt
    23401 tgctgctgct gtaattctgc tgactgcttc gccgcgtggt ggcgaatgct cgcgcgaagc
    23461 gccattgtgc tctgtaagcg agtatttact tcttcaatct gctggcgaat atgcgccagc
    23521 gccgcgctgt gttctgcgat gcgttcccag tgtggacgaa gatttcgtgc cggttgtgcc
    23581 agactaagcg ccgccagttg aggttgcgct ttttcttctt cggctaacgc ctgttgcaag
    23641 gcctgctgac ggcggctggc ttcttgctgc aattcgtcct gacgcgttaa ccagtttagc
    23701 gattgttgtt cttgctgctg cgcggtaatt aactgttttt cttcgtcagt aagtacctgc
    23761 aaactcgctg tcagcgattg cacttgttcc ggcgtgagca acgtgacgcc gctggcctgc
    23821 gcttgcagct tctccagctc tgtgcgggcc gatttgtgct gctcaaaaac catcgccgag
    23881 atttgcccgt agatttcagt gccggttaac tcctcgagca attccgcgcg ttctttgggt
    23941 ttggcattca ggaaggcagc aaattgcccc tgcgaaagca gcatcgaacg ggtgaagcgc
    24001 ccgtaatcca gcccggttaa cgtcgctgtc agttccagct tatctttcac tttgtcggcg
    24061 agaattttgc cgtcggcgca gcgcgccagc tctacgcgtg gcacctgcaa attaccgtcg
    24121 ggttggttac gcgcccgatt ctggctccag aatgcacggt acgcttcacc tttcacttca
    24181 aactccacct ccgccagaca ttcggcggta tcgcgggtca tgagatcatt ttgcgattgt
    24241 gaaacgttag agagacgcgg agtttcgtga tacagcgcca gacaaatggc gtccagcagg
    24301 gtggttttcc ccgcacctgt tgggccggta atagcaaaca gcccgttgct ggcgaacggc
    24361 tcgcgggtga aatcaatctt ccattcgcct tttaatgagt tcaggttttt caggcgcagg
    24421 ctgagaattt tcatgcttcg tgttctccgg cgagggtatg caacgtcgtg gtgaaaagat
    24481 gctgcagacg ttgctgctgc gattcatcca gttcttccag tgccagacgg cgattgaaca
    24541 cctcttcgac gctgagttcg ctgagggttt cacgctgttg gctggctaac acacgctcgc
    24601 gctgttcacg actccgacgt accagcaata cttcgacagg caatgattcg gttaatgcct
    24661 ggattttgcg ctgaatatca tgcagatact catcagtagt gatttcgata tccagccaga
    24721 caggtggctc ctgcgataca tcgcgccact gttccagctg tgcggtaatc gacgccagat
    24781 cgcctttcag cactgccatg ggttgcgtta ccggtacgtt caggttttcc acgctctcta
    24841 atttgccgtt tgaaaatgtc accagatgga catatttact cttaccgcat tcatcaaaac
    24901 tcagtggaat gggggagccg caatagcgaa catgttccat gccgccaata atctgtgcgc
    24961 ggtgaatatg cccgagcgcg atgtagtcgg ctggtggaaa gttttgtgcc ggaaacgcgt
    25021 ccagcgtgcc aatataaatg tcacgcacgg cgtcactttt actggccccc acggtcgtta
    25081 aatgtcccgt ggcgatgatg ggcagaggct gatcgccgcg cagtttgcag gcatcggcat
    25141 agtgttgttg gtaataatcg gtaatcgctg ccagtaaatg ctgctgtttt tcaataccgt
    25201 taagccccgc ctggctggta ataatgtcac gcggacgtaa aaacggaatg gggcacagca
    25261 ctgcgcctgg cgtcccgtcg cgacgaggca agatttgcgg cgcatgtccg gcgctggcga
    25321 ccacggtcgt attgaggaac gccatgatat cgcgcgattc attcagcgtg gcgaccgagt
    25381 catggtttcc tgccagtacc accagatgac agccagtttg ctgtaaattg acaacaaaac
    25441 ggttgtataa cgtgcgggcg taactgggcg gcgagccggt atcgaaaaca tcaccagcaa
    25501 caataatcgc atccacctga tgggtttgtg ctgtttccag cagccagtca agaaaagcct
    25561 gatgttcagc ttcgcggctt ttactgtaga agttctggcc gagatgccag tctgaggtgt
    25621 gaaggatgcg cataacggtt ccctggcgaa aaagcatggg cgcgattata cccaaacaga
    25681 tgtgccattt gcttttttct gcgccacgga aatcaataac ctgaagatat gtgcgacgag
    25741 cttttcataa atctgtcata aatctgacgc ataatgacgt cgcattaatg atcgcaacct
    25801 atttattaca acagggcaaa tcatggcgag acgtattctg gtcgtagaag atgaagctcc
    25861 aattcgcgaa atggtctgct tcgtgctcga acaaaatggc tttcagccgg tcgaagcgga
    25921 agattatgac agtgctgtga atcaactgaa tgaaccctgg ccggatttaa ttctccttga
    25981 ctggatgtta cctggcggct ccggtatcca gttcatcaaa cacctcaagc gcgagtcgat
    26041 gacccgggat attccagtgg tgatgttgac cgccagaggg gaagaagaag atcgcgtgcg
    26101 cggccttgaa accggcgcgg atgactatat caccaagccg ttttcgccga aggagctggt
    26161 ggcgcgaatc aaagcggtaa tgcgccgtat ttcgccaatg gcggtggaag aggtgattga
    26221 gatgcagggg ctaagcctcg acccgacatc tcaccgggtg atggcgggcg aagagccact
    26281 ggagatgggg ccgacagaat ttaaactgct gcactttttt atgacgcatc ctgagcgcgt
    26341 gtacagccgc gagcagctgt taaaccacgt ctggggaact aacgtgtatg tggaagaccg
    26401 cacggtcgat gtccacattc gtcgcctgcg taaagcactg gagcccggcg ggcatgaccg
    26461 catggtgcag accgtgcgcg gtacaggata tcgtttttca acccgctttt aacgccttgc
    26521 tcatcggacg cagagcaggg cttatgattt cttaactgga gtatcttacg tgctggaacg
    26581 gctgtcgtgg aaaaggctgg tgctggagct gctactttgc tgcctcccgg ctttcatcct
    26641 gggtgcattt tttggttacc tgccctggtt tttgctggca tcggtaacgg gactgcttat
    26701 ctggcatttc tggaatttat tgcgcctttc atggtggctg tgggtggatc gtagtatgac
    26761 cccgccaccg gggcgtggta gctgggagcc gctactatac ggcttacacc agatgcagct
    26821 gcgaaataaa aaacgccgcc gtgaactggg caatctgatt aaacgctttc gtagcggcgc
    26881 ggagtcgctg cccgacgcag tggtgctgac cacggaagag ggcggtattt tctggtgtaa
    26941 cggtctggcg caacagattc ttggtttgcg ctggccggaa gataacgggc agaacatcct
    27001 taacctgctg cgttacccgg agtttacgca atatttgaaa acgcgtgatt tttctcgccc
    27061 gctcaatctg gtgctcaaca ccgggcggca tctggaaatt cgcgtcatgc cttataccca
    27121 caaacagtta ctgatggtgg cgcgtgatgt cacgcaaatg catcaactgg aaggggcgcg
    27181 gcgtaacttt tttgccaacg tgagccatga gttacgtacg ccattgaccg tgttacaggg
    27241 ttacctggag atgatggatg agcaaccgct ggaaggcgcg gtacgtgaaa aagcgttgca
    27301 caccatgcgc gagcagaccc agcggatgga agggctggtg aagcaattac tgacgctgtc
    27361 gaaaatagaa gccgcaccga cgcatttgct caatgaaaag gttgatgtgc cgatgatgct
    27421 gcgcgttgtt gagcgcgagg ctcagactct gagtcagaaa aaacagacat ttacctttga
    27481 gatagataac ggcctcaagg tgtctggcaa cgaagatcag ctacgcagtg cgatttcgaa
    27541 cctggtctat aacgccgtga atcatacgcc ggaaggtacg catatcaccg tacgctggca
    27601 gcgagtgccg cacggtgccg aatttagcat tgaagataac ggaccgggca ttgcaccgga
    27661 gcatattccg cgcctgaccg agcgttttta tcgcgttgat aaagcgcgtt cccggcaaac
    27721 cggcggtagc ggattagggt tagcgatcgt gaaacatgcg gtgaatcatc acgaaagtcg
    27781 cctgaatatt gagagtacag taggaaaagg aacacgtttc agttttgtta tcccggaacg
    27841 tttaattgcc aaaaacagcg attaatccgc ctttgtcatc ttttattgcc ataagccagc
    27901 cgatgctggc ttattttctt tgcagtcaaa atatgggcgt tagattttac aacggttggt
    27961 gattttttgt tcgcatgatt agccatgtct ttttcacgga aatagtgttt tatactggtt
    28021 ggtgatttct tatcgctata tacctctggt ttttagatcc ctccttgctt taaaacgtta
    28081 taagcgttta aattgcgctt caggtgctgt catactgact gcattaacgc ggtaaatcga
    28141 aaaactattc ttcgccgcgc ctggttggga gtatttcccg ctaaaattgt ttaaatatac
    28201 cgctgtatca tccccaggga ttggcacaaa aatttaacgt tacaacacca catccacagg
    28261 cagtatgatt tatgacccat caattaagat cgcgcgatat catcgctctg ggctttatga
    28321 catttgcgtt gttcgtcggc gcaggtaaca ttattttccc tccaatggtc ggcttacagg
    28381 caggcgaaca cgtctggact gcggcattcg gcttcctcat tactgccgtt ggcctgccgg
    28441 tattaacggt agtggcgctg gcaaaagttg gcggcggtgt tgacagcctc agcacgccaa
    28501 tcggtaaagt cgctggcgta ctgctggcaa cggtttgtta cctagcggtg gggccgcttt
    28561 tcgctacgcc gcgtacagct accgtttctt ttgaagtggg gattgcgccg ctgacgggtg
    28621 attccgcgct gccgctgttt atctacagcc tggtctattt cgctatcgtt attctggttt
    28681 cgctctatcc gggcaagctg ctggataccg tgggcaactt ccttgcgccg ctgaaaatta
    28741 tcgcgctggt catcctgtct gttgccgcga ttgtctggcc ggcgggttct atcagcacgg
    28801 cgactgaggc ttatcaaaac gctgcgtttt ctaacggctt cgttaacggc tatctgacca
    28861 tggatacgct gggcgcaatg gtgtttggta tcgttattgt taacgcggcg cgttctcgtg
    28921 gcgttaccga agcgcgtctg ctgacccgtt ataccgtctg ggctggcctg atggcgggtg
    28981 ttggtctgac tctgctgtac ctggcgctgt tccgtctggg ttcagacagc gcgtcgctgg
    29041 tcgatcagtc tgcaaacggc gctgctattc tgcatgctta cgttcagcac acctttggcg
    29101 gtggcggtag cttcctgctg gcggcgttaa tcttcatcgc ctgcctggta acggcagttg
    29161 gcctgacctg tgcttgtgca gaattctttg cccagtacgt accgctctct tatcgtacgc
    29221 tggtgtttat cctcggcggc ttctcgatgg tggtttctaa cctcggctta agccagctga
    29281 tccagatctc cgtaccggtg ctgaccgcta tttatccgcc gtgtatcgca ctggttgtat
    29341 taagttttac acgctcatgg tggcataatt cgtcccgcgt gattgctccg ccgatgttta
    29401 tcagcctgct ttttggtatt ctcgacggga tcaaagcatc tgcattcagc gatatcttac
    29461 cgtcctgggc gcagcgttta ccgctggccg aacaaggtct ggcgtggtta atgccaacag
    29521 tggtgatggt ggttctggcc attatctggg atcgcgcggc aggtcgtcag gtgacctcca
    29581 gcgctcacta aatcactgaa catttgtttt aaccacgggg ctgcgatgcc ccgtggtttt
    29641 ttattgtgtt gatgggttag gaattgatgg aaagtaagaa caagctaaag cgtgggctaa
    29701 gtacccgcca catacgcttt atggcactgg gttcagcaat tggcaccggg ctgttttacg
    29761 gttcggcaga cgccatcaaa atggccggtc cgagcgtgtt gttggcctat attatcggtg
    29821 gtatcgcggc gtatatcatt atgcgtgcgc tgggggaaat gtcggtacat aacccggccg
    29881 ccagctcttt ctcgcgttat gcgcaggaaa acctcggccc gctggcaggt tacattaccg
    29941 gctggaccta ctgctttgaa atcctaattg tcgccatcgc cgatgttacc gcttttggta
    30001 tctatatggg tgtctggttc ccgacggtgc cgcactggat ttgggtactg agcgtggtgc
    30061 tgatcatttg cgccgtaaac ctgatgagcg tgaaggtatt cggtgagctg gaattctggt
    30121 tctcgttctt taaagtcgcc actatcatca tcatgattgt cgccggtttc ggcatcatca
    30181 tctgggggat tggcaacggc gggcaaccga ccggtattca taacctgtgg agcaacggcg
    30241 gcttcttcag taacggctgg cttggcatgg taatgtcgtt gcaaatggtg atgtttgctt
    30301 acggtgggat cgaaattatc gggattaccg ccggtgaagc gaaagatcct gagaaatcga
    30361 taccgcgtgc gattaactcc gtgccgatgc gtattctggt gttctacgtc ggtacgctgt
    30421 tcgtcattat gtctatctac ccgtggaatc aggttggcac tgccggtagc ccgttcgtgc
    30481 tgacgttcca gcatatgggc attacctttg ccgccagcat tcttaacttt gttgtgctga
    30541 ctgcttcgct gtcggcaatt aacagtgacg tatttggcgt aggccgtatg ctccacggta
    30601 tggcagagca gggcagcgcg ccgaaaattt tcagcaaaac gtcgcgtcgc ggtattccgt
    30661 gggttacggt gctggtgatg actaccgcgc tgctgtttgc ggtgtatctg aactacatca
    30721 tgccggaaaa cgtcttcctg gtgatcgctt cgctggcaac cttcgccacg gtgtgggtgt
    30781 ggattatgat cctgctgtcg caaattgcct tccgtcgccg tttgccgcca gaagaagtta
    30841 aggcgctgaa atttaaagtg ccgggtgggg tagcaacgac catcggcggg ctgattttcc
    30901 tgctctttat tatcgggttg attggttatc acccggatac gcgtatctcg ctgtatgtcg
    30961 gtttcgcgtg gattgttgtg ctgttgattg gctggatgtt taaacgccgc cacgatcgtc
    31021 agctggctga aaaccagtaa tccttgcgtt ctgtaagccg gttcaggagg aaatcctgat
    31081 ccggctattc cgaaagttat ccgcccccgt cctcctcccc caaatatcct tcagatgatg
    31141 agtgatcctg cattaggcta tggcaaggtg atcagatttt catcacaggg gaattatgat
    31201 gttaaatgca tggcacctgc cggtgccccc atttgttaaa caaagcaaag atcaactgct
    31261 cattacactg tggctgacgg gcgaagaccc accgcagcgc attatgctgc gtacagaaca
    31321 cgataacgaa gaaatgtcag taccgatgca taagcagcgc agtcagccgc agccaggcgt
    31381 caccgcatgg cgggcggcga tcgatctctc cagcggacaa ccccggcggc gttatagttt
    31441 caaattgctt tggcacgatc gccagcgttg gtttacaccg cagggcttca gccgaatgcc
    31501 gccggcacga ctggagcagt ttgccgttga tgtaccggat atcggcccac aatgggctgc
    31561 ggatcagatt ttttatcaga tcttccctga tcgtttcgcc cgtagtcttc ctcgtgaagc
    31621 tgaacaggat catgtctatt accatcacgc agccggacaa gagatcatat tgcgtgactg
    31681 ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc tatggcggcg atctggacgg
    31741 gataagcgaa aaactgccgt atctgaaaaa acttggcgtg acggcgctgt atctcaatcc
    31801 ggtgtttaaa gctcccagcg tacataaata cgataccgag gattatcgcc atgtcgatcc
    31861 gcagtttggc ggtgatgggg cgttgctgcg tttgcgacac aatacgcagc agctgggaat
    31921 gcggctggtg ctggacggcg tgtttaacca cagtggcgat tcccatgcct ggtttgacag
    31981 gcataatcgt ggcacgggtg gtgcttgtca caaccccgaa tcgccctggc gcgactggta
    32041 ctcgtttagt gatgatggca cggcgctcga ctggcttggc tatgccagct tgccgaagct
    32101 ggattatcag tcggaaagtc tggtgaatga aatttatcgc ggggaagaca gtattgtccg
    32161 ccactggctg aaagcgccgt ggaatatgga cggctggcgg ctggatgtgg tgcatatgct
    32221 gggggaggcg ggtggggcgc gcaataatat gcagcacgtt gccgggatca ccgaagcggc
    32281 gaaagaaacc cagccggaag cgtatattgt cggcgaacat tttggcgatg cacggcaatg
    32341 gttacaggcc gatgtggaag atgccgccat gaactatcgt ggcttcacat tcccgttgtg
    32401 gggatttctt gccaataccg atatctctta cgatccgcag caaattgatg cccaaacctg
    32461 tatggcctgg atggataatt accgcgcagg gctttctcat caacaacaat tacgtatgtt
    32521 taatcagctc gacagccacg atactgcgcg atttaaaacg ctgctcggtc gggatattgc
    32581 gcgcctgccg ctggcggtgg tctggctgtt cacctggcct ggtgtaccgt gcatttatta
    32641 cggtgatgaa gtaggactgg atggcaaaaa cgatccgttt tgccgtaaaa cgttcccctg
    32701 gcaggtggaa aagcaggata cggcgttatt cgcgctgtac cagcgaatga ttgcgctgcg
    32761 taagaaaagt caggcgctac gtcatggcgg ctgtcaggtg ctgtatgcgg aagataacgt
    32821 ggtggtattt gtccgcgtgc tgaatcagca acgtgtactg gtggcaatca accgtggcga
    32881 ggcctgtgaa gtggtgctac ccgcgtcacc gtttctcaat gccgtgcaat ggcaatgcaa
    32941 agaagggcat gggcaactga ctgacgggat tctggctttg cctgccattt cggctacggt
    33001 atggatgaac taacgtttta taacgccttg cgtgacgcct gcgccatcat ccgcggataa
    33061 aactgccaga agcgggtttc gagggcgtca taatgagcgt ctaaatcgta ccaggagtca
    33121 cgcagggcat ccagacgtgg gcggcggctt gccatgccgt ttaacacgtt ctggatgaaa
    33181 tccatatcgc gatagcgcac cagccactgc tctgaccaca agtaattgtt cagattgata
    33241 aaacgtggcg gtgagtccgg caaaatcgtc atcacttgct cgcgggcata acagacaaat
    33301 tcctgtagcg gaaagtccgg cgacagctgc gaccagtggc gggaaagaaa gtgatcccac
    33361 atgacatcca gcgtaatagg cgcaacgcgg cgcgtttcac tacgaaacca ctcccgtgct
    33421 tcgcggactt ccggcagatt gtcagtcaat acgtcgatac gtcgatgcat atgaatgcca
    33481 gccacgacgt cgggcggaaa actttcttca ggatttccgc gtacgaaatc agccagtaaa
    33541 ttgccggaaa gcgagctttc cgcgagatgg gctaaatgca ggtgagctaa aaaattcatc
    33601 gattatattc tatccaaaag ggggtaaagg ttgcagggag agcgccccgg cactagacta
    33661 cccgcctctt attttagtct gagtcagtgt catgcgcgtt accgatttct cctttgaatt
    33721 gcccgaatcc ctgattgccc actatcccat gcctgaacgc agtagctgtc gtttactgtc
    33781 gctggacggg ccgacgggcg cgctgacgca cggtactttc accgatttac ttgataagct
    33841 caaccccggc gatcttctgg tttttaataa tacccgcgtg atcccggcgc gcctgtttgg
    33901 gcgtaaagcc agcggcggca agattgaagt gctggttgaa cggatgctcg acgacaaacg
    33961 cattcttgcg catattcgcg cctcgaaagc gccaaaacct ggcgcagaac tgctgctggg
    34021 cgatgacgaa agtattaacg caacaatgac cgcgcgccac ggcgcactgt ttgaagtcga
    34081 atttaatgat gaacgctcgg tgctggatat tctcaacagc atcggccata tgccgctgcc
    34141 gccgtatatc gaccgtccgg acgaagacgc tgaccgcgaa ctttatcaaa ccgtttatag
    34201 cgaaaaaccg ggcgcggttg cagccccgac cgcaggtctg cattttgacg agcctttgct
    34261 ggaaaaattg cgcgccaaag gcgtggagat ggcgtttgtg acgttgcacg ttggtgcggg
    34321 caccttccag ccggtgcgcg tcgacaccat tgaagatcac atcatgcact cggaatacgc
    34381 tgaagtaccg caggatgtgg tagacgcggt actggcggcg aaagcgcgcg gtaaccgggt
    34441 gattgcggtt ggcaccactt cagtacgttc gctggaaagc gcggctcagg cagcgaaaaa
    34501 cgatctcatt gaaccgttct tcgacgatac ccaaatcttt atctatccgg gcttccagta
    34561 caaagtggtc gatgcgctgg tgacgaactt ccacttgcca gagtcgacgc tgattatgct
    34621 ggtttcggcc tttgccggtt atcaacacac catgaacgcc tataaagcag cggtagaaga
    34681 gaaatatcgc ttttttagtt acggtgatgc gatgtttatc acgtacaatc cgcaggcaat
    34741 taatgagcgc gtcggggagt aattccgcgg cgctggttta aaacgttgga ctgtttttct
    34801 gacgtagtgg agaaaaaatg aaatttgaac tggacaccac cgacggtcgc gcacgccgtg
    34861 gccgcctggt ctttgatcgt ggcgtagtgg aaacgccttg ttttatgcct gttggcacct
    34921 acggcaccgt aaaagggatg acgccggaag aagttgaagc cactggcgcg caaattatcc
    34981 tcggcaacac cttccacctg tggctgcgcc cgggccagga aatcatgaaa ctgcacggcg
    35041 atctgcacga ttttatgcag tggaaggggc cgatcctcac cgactccggc ggcttccagg
    35101 tcttcagcct tggcgatatt cgtaaaatca ccgaacaggg cgtgcacttc cgtaacccga
    35161 tcaacggcga tccgattttc ctcgatcctg aaaaatcaat ggagattcag tacgatcttg
    35221 gttcggatat cgtcatgatc tttgatgagt gtacgccgta tcctgctgac tgggattacg
    35281 caaaacgctc catggagatg tctctgcgtt gggcgaagcg tagccgtgag cgttttgaca
    35341 gtctcggaaa caaaaatgcg ctgtttggta tcatccaggg cagcgtttac gaagatttac
    35401 gtgatatttc tgttaaaggt ctggtagata tcggttttga tggctacgct gtcggcggtc
    35461 tggctgtggg tgagccgaaa gcagatatgc accgcattct ggagcatgta tgcccgcaaa
    35521 ttccggcaga caaaccgcgt tacctgatgg gcgttggtaa accagaagac ctggttgaag
    35581 gcgtacgtcg tggtatcgat atgtttgact gcgtaatgcc aacccgcaac gcccgaaatg
    35641 gtcatttgtt cgtgaccgat ggcgtggtga aaatccgcaa tgcgaagtat aagagcgata
    35701 ctggcccact cgatcctgag tgtgattgct acacctgtcg caattattca cgcgcttact
    35761 tgcatcatct tgaccgttgc aacgaaatat taggcgcgcg actcaacacc attcataacc
    35821 ttcgttacta ccagcgtttg atggcgggtt tacgcaaggc tattgaagag ggtaaattag
    35881 agagcttcgt aactgatttt taccagcgtc aggggcgaga agtaccacct ttgaacgttg
    35941 attaatatta ataatgaggg aaatttaatg agctttttta tttctgatgc ggtagcggca
    36001 acgggtgcac cggcgcaagg tagcccgatg tctttgattt tgatgctggt ggtattcggt
    36061 ctgattttct atttcatgat cctgcgtcca cagcagaagc gcaccaaaga acacaaaaag
    36121 ctgatggact ccattgccaa aggtgatgaa gttctgacga acggtggcct ggttggtcgc
    36181 gtaaccaaag tagcggaaaa cggctacatt gctatcgcgc tgaatgacac cactgaagta
    36241 gttattaaac gtgacttcgt agctgccgtc ctgccgaaag gcaccatgaa ggcgctgtaa
    36301 ttaaaatttt tccctaaggg aattgccgtg ttaaaccgtt atcctttgtg gaagtacgtc
    36361 atgctgatcg tggtgattgt catcggtctg ctgtatgcgc ttcccaacct gtttggtgag
    36421 gatccggctg ttcagatcac tggtgcgcgc ggagtcgccg ccagtgagca aacgctgatc
    36481 caggtccaga aaacgttaca agaagaaaaa ataactgcta agtctgtggc actggaagag
    36541 ggcgctattc ttgcgcgctt cgactccact gacacccagc tgcgcgctcg tgaagcatta
    36601 atgggcgtta tgggtgacaa atacgtcgtg gcgcttaacc ttgccccggc aacgccgcgc
    36661 tggctggcag ctattcacgc tgagccgatg aagctcggcc ttgacctgcg tggcggcgtt
    36721 cacttcctga tggaagtgga tatggacacc gcgcttggca aactccagga acaaaatatc
    36781 gatagcctgc gcagcgatct gcgtgaaaag ggcatcccgt ataccaccgt tcgtaaagaa
    36841 aacaactacg gcctgagcat tactttccgc gatgctaaag ctcgtgatga agccattgcg
    36901 tatctgagca agcgccatcc ggacctggtg attagtagcc agggcagcaa ccagctgcgt
    36961 gcggtaatga gcgatgctcg tctgagtgaa gcgcgtgaat atgcggtgca gcagaacatc
    37021 aatatcctgc gtaaccgtgt aaaccaactt ggcgtggcgg agccggtggt tcagcgtcag
    37081 ggtgctgacc gtatcgttgt tgaactgcca ggtattcagg acactgcgcg tgcgaaagag
    37141 attctgggtg cgacggcaac gctggaattc cgtctggtaa acaccaacgt tgaccaggcc
    37201 gctgcggcat ccggtcgcgt accgggcgac tctgaagtga aacagacccg cgaaggtcag
    37261 ccagttgtgc tgtacaaacg cgtaattctg accggtgacc atatcaccga ctccacttcc
    37321 agccaggacg aatacaacca accgcaggtt aacatctcgc tcgatagcgc tggtggtaac
    37381 atcatgtcta acttcactaa ggacaacatc ggcaaaccga tggcaaccct gtttgtggag
    37441 tacaaagaca gcggtaagaa agatgcaaat ggtcgtgcgg ttctggtgaa acaggaagag
    37501 gtgattaaca tcgccaacat ccagtctcgt ctgggtaaca gcttccgtat caccggcatc
    37561 aacaacccga acgaagcccg tcagctgtca ctgctgctgc gtgccggtgc gttgatcgcg
    37621 ccgattcaga ttgttgaaga acgtaccatt ggcccgaccc tgggtatgca gaacattgag
    37681 caggggctgg aggcttgcct tgccggtctg ctggtttcta ttctgttcat gatcatcttc
    37741 tataagaaat ttggtctgat tgcgaccagt gctctgattg ccaacttgat cttgatcgtc
    37801 ggcattatgt cgctgttgcc aggcgcaacg ctgagtatgc caggtatcgc gggtatcgtc
    37861 ttaacacttg cggtagcggt cgatgcgaac gtacttatca acgaacgtat taaagaagag
    37921 ttgagcaacg gacgtactgt tcaacaggca attgatgaag gttatcgtgg cgcattcagt
    37981 tctatcttcg atgcgaacat caccacgctg attaaagtca tcatcctgta cgcagtgggt
    38041 accggggcaa ttaaagggtt cgcgattact accggtatcg gtgtggcgac gtcgatgttt
    38101 accgcgattg tcggtacgcg tgccatcgta aacctgctat atggcggcaa gcgcgtcaag
    38161 aagctgtcaa tctgaggagt gcgatgtggc acaggaatat actgttgaac aactaaacca
    38221 tggccgtaaa gtctatgact ttatgcgctg ggactactgg gctttcggca tctctggtct
    38281 gctgttaatc gccgctatcg ttattatggg cgtgcgcggc tttaactggg ggctggattt
    38341 caccggtggt acggttattg aaattacgct cgaaaaaccg gctgaaattg acgtaatgcg
    38401 tgatgcattg caaaaagccg gttttgaaga gccgatgctg caaaactttg gtagcagcca
    38461 tgacatcatg gtccgtatgc cgcctgctga aggcgaaacc ggcggtcagg tgttgggcag
    38521 ccaggttctg aaggtgatta acgaatccac caatcagaat gcagcagtga agcgtattga
    38581 gttcgtcggt ccgagcgtgg gggcagacct tgcgcaaacc ggtgcgatgg cgttgatggc
    38641 agcgctgctg tctatcctcg tgtacgtagg tttccgcttt gagtggcgac tggcggcagg
    38701 ggtggttatt gcgctggcgc acgacgttat cattacgctg ggtattttgt cgttattcca
    38761 tatcgagatt gacctgacca ttgtggcatc gttgatgtcg gttatcggtt actcgcttaa
    38821 cgacagtatc gtggtatcgg accgtattcg tgaaaacttc cgcaagatcc gtcgcggtac
    38881 gccttacgaa atctttaacg tgtccttgac ccagacgctg caccgtacct tgatcacatc
    38941 cggtactacc ttgatggtta tcctgatgct gtacctcttc ggtggtccgg tactggaagg
    39001 cttctcgctg accatgctta tcggtgtttc catcggtact gcatcttcca tctatgtggc
    39061 atctgcgttg gctctgaaac tgggtatgaa gcgcgaacac atgttgcagc agaaagtgga
    39121 aaaagaaggg gcggatcagc cgtcaattct gccgtaatca agttcccgtt gatgttgaaa
    39181 atcccggtca gaagatcggg attttttttg atgtatggag attgcgaaaa tccccgcatc
    39241 ttgggaaact gcgcgtaacc ctacatttca tccaggtaac ttttcatggc tatcatccca
    39301 aaaaactatg cgcggttaga aagcggctat cgcgaaaaag cattaaaaat ctatccgtgg
    39361 gtctgcggtc gctgttcccg cgagtttgtt tattccaacc tgcgtgaact taccgttcac
    39421 cacattgatc acgaccatac caataacccg gaagatggca gtaactggga attgttgtgt
    39481 ctctattgcc acgatcatga gcattcgaaa tataccgaag cggatcagta tggtacgacc
    39541 gttatcgcag gggaagatgc gcagaaagat gtcggtgaag cgaagtacaa cccattcgct
    39601 gacctgaaag cgatgatgaa caagaagaag tgattaaaac gtaaaattgc ctgatgcgct
    39661 acgcttatca ggcctacgtt atttcagcaa tatattgaat ttacgtgctt ttgttggccg
    39721 gacaaagcgt ttacgccgca tccggcatga acaaagcaca cgttgttaac aatcagaaat
    39781 gccgggaata aatcccggca ttttcataat cagaagttgt aacctactac caggtaacca
    39841 ccccagccgg tagagcgaac gttgaagttg ccgttgccga agttcagttc tgcatcgtcg
    39901 ttccactgac caccgtcgtg ccagtaacga gctacgacag agtagtgcca gtgatcgtag
    39961 ttcagagcca gaatatggct ggaagcgata gagttattag tacgggtctt aataccgttg
    40021 attgcgttac cgctgtcatc ccctaaatcg gaaccccagt cgaagttggt gaagccgatg
    40081 tagctcagct gaccgcccca cagatcggta atcggcacaa agtatttaat tttgaaacgg
    40141 taaccgtccc actcgttttc gttcgctgcg ccatagttct gccactggta tttcgcatag
    40201 acgttcatgg acaggctcat cggcaggcca gtgtcgatat cggtacccag acccatgtac
    40261 caggtgctct ggcgaccatc tttattacga cccatgtcgt aaatgtagtt gttcgcgaag
    40321 taccactctt tgaacggacc gaagctaagg tcagtattgg tcagcttgtc gatggagaaa
    40381 cgtggttcga tttccataaa cagcggagaa ccgtggttcc agataccttt tgcatcggag
    40441 ttaccgccga agaataccgg cgcatccgca taaccataga agtcgaacca gtctttttta
    40501 gcgaatgctt cgtactcaag gtaggtatcg ttgcggatct gcggtccgaa acgggtgtga
    40561 tagcttccga caacgttaac gctctggtgc caccagtcgg aaagatactg cggtttgtcg
    40621 ttttcagctg cgttgacagt aaaagacgaa gagagcgcca gtaccgcacc ggctgccagt
    40681 aatgtttttt tcatatgtat gccactgttt gaaaatccct tgcgggagtg aaaaaggcgc
    40741 aaattgcgtt tctaaatatt tcgtatacag aggagcctat tataaaaatc attgctcaca
    40801 aaaatatgtt tcgtttcaca gttctatcat ttacgtaatc gattgcgttc acgtttacac
    40861 acattcgggc agggattgta ctgactttca cccttgttgc aaacttttac tatcaatctg
    40921 ggtgatgtaa gcggagtaac aaaatgacgg ggaaagatgt tgcgggacgc acaaaattgc
    40981 cccgcaatac gtttaatgca taacaggttg aatgtcgtgg atacgtacaa agcctaattg
    41041 atccacagaa atccccttta actgtaacgg aatatcaaca tcgctggggg caaggacgct
    41101 ggcaggggca ttaatcaatt gattctgcac attgatttcc tgaaagttgt ctattgttcc
    41161 ctgaatttgc ccatattcaa cagttccgct gaatgcgggc aagggatcat tcgactcgcc
    41221 ctgaatacgt agcgtcaggg tggtgccatc tgtatcaggc gtaatattca ccagcgacat
    41281 acgtaaagta ccgatctggc tttccagtct tgccggtgtt ttcgctccag gcagaaggta
    41341 aaccccactg ctggattttg cattcagcct gttttgctga gtgattttga ctgtttcttg
    41401 attgagttgc gtcatctcct ggttcagcgt gctgacgctg tgtttcatct gtcgcacttc
    41461 actttgctgc acacaggcgc taaggctgaa caggcttccc agcaggagca gtcgaaaaac
    41521 gttagtattc ataggtcgtc ccttgctcgt cgcaattagc ctaatggtag agggtaaaag
    41581 cagcgaaagc atcgatcctt tatctcaaaa gcgttgcgcc tttgttgtat cgtcagttca
    41641 gggtaaaata gatttccgtt aaccacctgg tcaggacgcc gtatgcattg cccattctgt
    41701 ttcgccgtgg acactaaggt aattgactct cgtctcgtgg gcgagggttc atctgtacgc
    41761 cgccgtcggc agtgtctggt gtgtaatgaa cgtttcacca cctttgaagt ggcggagctg
    41821 gttatgccgc gtgttgtaaa aagcaacgac gtgcgtgaac cgtttaatga agagaaattg
    41881 cgtagcggaa tgctgcgggc gctggaaaaa cgtccggtga gttccgatga cgtcgaaatg
    41941 gcaatcaatc atattaaatc gcagctgcgc gccaccggtg agcgcgaagt gccgagcaag
    42001 atgattggca atctggtgat ggagcaattg aaaaagctcg ataaagtcgc ctatatccgt
    42061 tttgcctctg tctaccgcag tttcgaagat atcaaagaat ttggcgaaga gatcgcgcgc
    42121 ctggaggact aagccgtgca ggacgagtat tacatggcgc gggcgcttaa gctggcgcag
    42181 cgggggcgtt ttaccactca tcccaacccg aatgttgggt gtgtcattgt gaaagatggc
    42241 gaaatcgtcg gtgaaggtta ccatcaccgt gctggtgaac cacacgccga agtacatgcg
    42301 ttgcgtatgg ctggagaaaa agccaaaggt gcgactgcgt atgtgacact cgaaccttgc
    42361 agccatcatg gccgtacgcc gccttgctgt gatgcattaa ttgccgcagg tgtggcgcgc
    42421 gtggttgcct cgatgcaaga tcctaacccg caggtagccg ggcgagggct atatcgcctg
    42481 caacaagcgg gtattgacgt cagccacggc ctgatgatga gtgaagccga acaattgaat
    42541 aaaggctttc tcaagcggat gcgtaccggc tttccttata tccagttaaa acttggcgca
    42601 tcgcttgatg gtcgtacggc gatggccagc ggtgagagcc agtggattac ttcacctcag
    42661 gcgcggcgcg atgtgcaacg cctgcgcgcg caaagtcatg ccattttaac cagtagcgct
    42721 acagtactgg cagacgatcc ggccttaacg gtgcgttggt ctgaactgga tgaacaaact
    42781 caggcacttt atccgcaaca aaatctacgc cagccggtac gtattgtcat tgatagccaa
    42841 aatcgtgtga cgcctgaaca tcgtatcgtg cagcagcctg gcgaaacctg gtttgcgcgt
    42901 acacaggaag attctcgtga atggccggaa acagttcgta gtttgctcat cccggaacat
    42961 aacggacatc tggatttggt gctgctcatg atgcaactgg gtaaacagca gattaacagc
    43021 atctgggtgg aagcggggcc aacgctcgct ggcgcattgc tgcaggcggg tttagtcgat
    43081 gagctgattg tctatatcgc acctaaacta ttaggcagcg acgcccgtgg attatgctcg
    43141 ctgccagggc ttgagaaatt agccgacgcc ccccaattta aattcaaaga gatacgtcat
    43201 gtaggcccgg atgtttgcct gcatttagtg ggtgcatgat ctcccggctc gaaagggaag
    43261 cagcgcacaa aatattatgc taaaatccgc ccccctgcgg ggccatactc gaacccgaag
    43321 gaagaaaatg aacattattg aagctaacgt tgctaccccg gacgctcgcg tcgccatcac
    43381 catcgcgcgt ttcaacaact ttatcaatga cagcctgctg gaaggtgcaa ttgatgcact
    43441 gaaacgtatc ggtcaggtaa aagatgaaaa cattaccgtt gtttgggtgc ctggtgccta
    43501 tgagctgccg ctggcggcgg gtgcactggc taaaaccggt aaatacgacg cggtgattgc
    43561 gctgggtacg gttattcgtg gtggcactgc ccattttgaa tatgtcgctg gtggtgcaag
    43621 caacggcctg gcgcatgttg cccaggacag cgaaattccg gttgcttttg gggttctgac
    43681 cactgaaagc attgaacaag cgatcgaacg tgctggcacc aaagctggca acaaaggtgc
    43741 agaagctgca ctgaccgcgc ttgaaatgat taatgtattg aaagccatca aggcctgaaa
    43801 ttagtaaggg gaaatccgtg aaacctgctg ctcgtcgccg cgctcgtgag tgtgccgtcc
    43861 aggcgctcta ctcctggcag ttgtcccaga acgacatcgc tgatgttgaa taccagttcc
    43921 tggctgaaca ggatgtaaaa gacgttgacg tcctgtactt ccgtgagctg ctggccgggg
    43981 tggcgactaa taccgcatac ctcgacggac tgatgaagcc atacctgtcc cgcctgctgg
    44041 aagaactggg acaggtagaa aaagcagtac tgcgcattgc gctgtacgaa ctgtctaaac
    44101 gtagcgatgt gccatacaaa gtggccatta acgaagcgat cgaactggcg aaatcgttcg
    44161 gcgcagaaga cagccataag ttcgtcaacg gcgtactcga taaagcagca cctgtgattc
    44221 gccctaacaa aaagtaatat ccaggccggt agattcacgg aagactgttc catgatcgcc
    44281 ggccttttct tttttacctg ctgaggcata acgtatggca tgtggcgagt tctccctgat
    44341 tgcccgttat tttgaccgtg taagaagttc tcgtcttgat gtcgaactgg gcatcggcga
    44401 cgattgcgct cttctcaata tccccgagaa acagaccctg gcgatcagca ctgatacgct
    44461 ggtggcgggc aaccacttcc tccctgatat cgatcctgct gatctggctt ataaagcact
    44521 ggcggtgaac ctaagcgatc tggcagcgat gggggccgat ccggcctggc tgacgctggc
    44581 attaacctta ccggacgtag acgaagcgtg gcttgagtcc ttcagcgaca gtttgtttga
    44641 tcttctcaat tattacgata tgcaactcat tggcggcgat accacgcgtg ggccattatc
    44701 aatgacgttg ggtatccacg gctttgttcc gatgggacga gccttaacgc gctctggggc
    44761 gaaaccgggt gactggatct atgtgaccgg tacaccgggc gatagcgccg ccgggctggc
    44821 gattttgcaa aaccgtttgc aggttgccga tgctaaagat gcggactact tgatcaaacg
    44881 tcatctccgt ccatcgccgc gtattttaca ggggcaggca ctgcgcgatc tggcaaattc
    44941 agccatcgat ctctctgacg gtttgatttc cgatctcggg catatcgtga aagccagcga
    45001 ctgcggcgca cgtattggcc tggcattgct gccgttttct gacgcacttt ctcgccacgt
    45061 tgaaccggaa caggcgctgc gctgggcgct ctctggtggc gaagattacg agttgtgttt
    45121 cacggtgccg gaactgaacc gtggcgcgct ggatgtcgct ttgggacatc tgggcgtacc
    45181 atttacctgt atcgggcaaa tgaccgccga tatagaagga ctctgttttg ttcgtgacgg
    45241 cgaacctgtc acgttagact ggaaaggata tgaccatttt gccacgccat aaagatgtcg
    45301 cgaaaagtcg cctgaagatg agtaatccgt ggcatctgct tgctgtcgga ttcggaagtg
    45361 gattaagccc gatcattcct gggacgatgg gatcgctggc agcgattccg ttctggtatc
    45421 tgatgacctt tttgccctgg cagctctatt cgctggtggt gatgctgggg atctgtatcg
    45481 gcgtctatct ttgtcatcaa acggcgaaag acatgggtgt gcacgatcat ggcagcattg
    45541 tctgggacga atttattggt atgtggatca cgctcatggc gctgccgacc aatgactggc
    45601 agtgggttgc cgccgggttt gtgattttcc gtattctgga tatgtggaag ccgtggccga
    45661 tccgctggtt tgatcgcaat gtgcatggcg gcatggggat catgatcgac gatattgtcg
    45721 ccggggtgat ttccgcaggc atcctgtatt ttatcggtca tcactggccg ctgggtattc
    45781 tgtcgtagtt gtgcaccgat gcctgatgtg acgcttgtca cgtctcatca ggcctggact
    45841 cttatttaaa tcctacgaca ggatgcggtt tatacggcgt ttccagttcg gcaatctgtt
    45901 ccggcttcaa agtgatatcc accgcgttca atagctcatc aagctgttct tcgcgcgaag
    45961 ttccgataat cggtgcggca atgcccggtt tactcaacaa ccaggccagc gcaacttgtg
    46021 ctcgtgtcgc ccccagttct tcactgacgc ctgttaaccg ctctgcgatc tgcgcgtcat
    46081 tttcatcgct ttctttatag agatttttcc ccacctcatc agacaccagt cgtgcggtag
    46141 tttctcccca cggacgcgtc agacggcccc ttgccagcgg gctccatgga attaccgcca
    46201 cgccctcctg ataacacagt ggtagcatct cgcgctcttc ttcacgataa atcagattgt
    46261 agtgatcctg catactgaca aactgcgccc agccgtgctg tttttggagt tccagtgcct
    46321 gagcaaactg cgaagcgtgc attgatgacg cgccgatata acgcgctttc ccggctttta
    46381 ccacgtcgtt gagggcttcc agcgtctctt cgatcgacgt gttgtaatcc cagcgatgaa
    46441 tttgcaggat atcgacataa tccatgccga gacgtcgcag gctgtcgtcg atagagcgca
    46501 aaatttgcgc acgggataat ccttccggta aatcaccaac gcgatggaac actttggtcg
    46561 caacgaccac gtcttcacga cgggcgaaat cccgcagtgc gcgaccgacg atctcttcgc
    46621 tgctgccgtc agaataactg ttggcggtat caaagaaatt tatgccgcct tccagtgcac
    46681 gtttaattat gggacggctg ctttcttccg gcagtgtcca tgcgtgatta ccgcgatctg
    46741 gctcgccaaa ggtcatacag ccgaggcaaa gtcgggaaac gcgaaggtcg gtttttccta
    46801 aggggttgta ttgcatgctg ccactcctgc tatactcgtc atacttcaag ttgcatgtgc
    46861 tgcggctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg cttcactcgt
    46921 ttgccgcctt cctgcaactc gaattattta gagtctatga ataatttctt aagcatagca
    46981 ggagtggagt agggattatg ccagccaggc cttgattttg gcttccatac cagcggcatc
    47041 gaggccgagt tcggcgcgca tttcttcctg agttccttgc ggaataaaga agtccggcag
    47101 gccaatgttc agcacgggta ctggtttacg atgggccatc agcacttcgt tcacgccgct
    47161 gcctgcgccg cccataatgg cgttttcttc tacggtgacc agcgcttcat ggctggcggc
    47221 catttccaga attaacgctt catcaagcgg tttcacaaaa cgcatatcga ccagcgtggc
    47281 gttcagcgat tcggcgactt tcgccgcttc tggcatcagc gtaccaaagt taaggatcgc
    47341 cagtttctcg ccacgacgct tcacaatgcc tttgccaatt ggtagttttt ccagcggcgt
    47401 cagttccacg ccgaccgcgt tgccacgcgg gtagcgcacc gctgacgggc catcgttata
    47461 gtgatagccg gtatagagca tctggcgaca ttcgttttca tcgctcgggg tcataatgac
    47521 catttccggt atgcagcgca ggtaagagag atcaaaagca ccctgatggg tttgaccgtc
    47581 agcaccaaca atgcccgagc ggtcgatggc gaacaggacc ggaagctttt gaatcgccac
    47641 gtcatgcagc acctgatcat aggcgcgttg caggaaagtg gagtaaatcg cgacaatggg
    47701 tttgtaccca ccaatcgcca gacccgcagc aaaggtcacc gcgtgttgct cggcaattgc
    47761 cacgtcgaag tagcgatccg ggaatttacg tgaaaactcg accatgccgg aaccttcacg
    47821 catcgccgga gtaatcgcca tcagcttgtt gtctttcgct gccgtttcgc acaaccagtc
    47881 gccaaagatt tttgaatagc tcggcaaacc gccgctactt ttcggcaaac aaccgctgga
    47941 gggatcaaat ttaggcacgg cgtggaaagt gattgggtct ttttctgccg gttcataacc
    48001 acgacctttt ttggtcatga tatgcaggaa ctgcgggcct ttcaggtcgc gcatgttctt
    48061 aagcgtggtg ataagcccca gcacatcgtg accgtcaacc gggccgatgt agttaaagcc
    48121 cagctcttca aacaacgtgc caggcactac catgccttta atatgttctt cggtgcgttt
    48181 gagcagctct ttaattggcg gaacgccaga gaaaactttt ttcccgcctt cgcgcagtga
    48241 agagtaaagc ttaccggaaa gcagctgcgc cagatggttg ttgagcgcgc cgacattttc
    48301 ggaaatcgac atttcattgt cgttgagaac caccagcata tcaggacgga tatcgcccgc
    48361 gtgattcatc gcttcaaacg ccatgcctgc ggtaatcgca ccatcgccga ttacacagac
    48421 ggtgcggcga tttttgcctt ctttctcggc agcaaccgca ataccaattc cggcactgat
    48481 ggaggttgat gaatgcccga cgcttaatac gtcatattcg ctttcgccgc gccacgggaa
    48541 cgggtgcaga ccgcctttct gacggatggt gccgattttg tcgcggcgtc cggtcaaaat
    48601 tttatgcgga taagcctgat gccccacatc ccaaatcaat tggtcaaacg gggtgttgta
    48661 gacatagtgc agcgccacgg tcagttcgac cgtgcccagc ccggaggcga agtgcccgct
    48721 ggaacggctc acgctgtcga gtaaatagcg gcgcagttcg tcgcagagtt tcggtagact
    48781 ctctttcggc aacagtcgta actcctgggt ggagtcgacc agtgccaggg tcgggtattt
    48841 ggcaatatca aaactcatca ggggcctatt aatacttatt gtttatttat tacgctggat
    48901 gatgtagtcc gctagcgctt ccagtgccga ggtatcgagt gactgttcag ccagttgttt
    48961 cagcgactga cgggcatcgt cgatcagatc ccgggctttc ttccgggctt gctcaagacc
    49021 cagaagtgca gggtaggtac ttttaccaag ttgctggtcg gcaccctggc gttttcccaa
    49081 cgttgcagta tctcccacca catccaggat gtcatcctga acctggaagg caaggccgat
    49141 gctctctgca tacttgtcga gtaccggcag agcacgacgt cctttatctc cggcgcttaa
    49201 tgcaccaagg cgaacggcgg cgcgaatcaa tgcgccggtt ttatgacgat gaatacgctc
    49261 aagcgcgtcc agaggtacgt gtttgccttc cgcgtctaaa tctaatgcct gaccaccgca
    49321 cattccggca ataccactgg cgctcgccag ttcagaaatc atcgaaattc tgtcgcggtc
    49381 cgacacttcc ggcatatcgg catcgcttaa aatcgagaac gccagcgttt gtaaagcgtc
    49441 gccagcgaga atcgcgtttg cttcgccaaa cttcacatgg caggttggca aaccgcgacg
    49501 cagatcgtca tcatccattg ccggtaaatc atcatgaatt aatgagtaag cgtggataca
    49561 ctcaacggcg gcagcgggtg cgtccagcgt gtttgtgcta acgccgaaca tatgaccggt
    49621 ggcataaacc aggaaaggtc gcaggcgctt accacctaat aatgcgccat actgcatggt
    49681 ttcgaccacg ggagtgttct gaaagggcag tggggcgata aaacggctca gcgcctggtt
    49741 ggcctgctta acgcaggctt cgagttgctg cggaaagtcc attactcatt gtccggtgta
    49801 aaaggggtta gagaggcgtc ttcattgtca gacagcagaa tttgtacgcg ctgttcggct
    49861 tgttgtaatt tggcctgccc ctgacgtgcc agctgcacgc cgcgttcgaa ctcgttcagc
    49921 gcctcttcca gcggcaggtc gccactttcc agacgggtta caatctgttc cagctcgctc
    49981 agcgcctttt caaagctggc gggcgcctca tttttcttcg gcataatgaa tgtctgactc
    50041 tcaatatttt tcgccccgtc atggtaacgg actcagggca aatagcaaat aacgcgcaat
    50101 ggtaaggtga tgtgcacagc aaagcgatgt tagtggtata cttccgcgcc tggatgcagc
    50161 cgcaggtgtg ggctgctgta tttttcccta tacaagtcgc ttaaggcttg ccaacgaacc
    50221 attgccgcca tgaagtttat cattaaattg ttcccggaaa tcaccatcaa aagccaatct
    50281 gtgcgcttgc gctttataaa aatccttacc gggaacattc gtaacgtttt aaagcactat
    50341 gatgagacgc tcgctgtcgt ccgccactgg gataacatcg aagttcgcgc aaaagatgaa
    50401 aaccagcgtc tggctattcg cgacgctctg acccgtattc cgggtatcca ccatattctc
    50461 gaagtcgaag acgtgccgtt taccgacatg cacgatattt tcgagaaagc gttggttcag
    50521 tatcgcgatc agctggaagg caaaaccttc tgcgtacgcg tgaagcgccg tggcaaacat
    50581 gattttagct cgattgatgt ggaacgttac gtcggcggcg gtttaaatca gcatattgaa
    50641 tccgcgcgcg tgaagctgac caatccggat gtgactgtcc atctggaagt ggaagacgat
    50701 cgtctcctgc tgattaaagg ccgctacgaa ggtattggcg gtttcccgat cggcacccag
    50761 gaagatgtgc tgtcgctcat ttccggtggt ttcgactccg gtgtttccag ttatatgttg
    50821 atgcgtcgcg gctgccgcgt gcattactgc ttctttaacc tcggcggcgc ggcgcatgaa
    50881 attggcgtgc gtcaggtggc gcattatctg tggaaccgct ttggcagctc ccaccgcgtg
    50941 cgttttgtcg ctattaattt cgaaccggtc gtcggggaaa ttctcgagaa aatcgacgac
    51001 ggtcagatgg gcgttatcct caaacgtatg atggtgcgtg ccgcatctaa agtggctgaa
    51061 cgttacggcg tacaggcgct ggtcaccggc gaagcgctcg gccaggtgtc cagccagacg
    51121 ctgaccaacc tgcgcctgat tgataacgtc tccgacacgc tgatcctgcg tccgctgatc
    51181 tcttacgaca aagagcacat catcaacctg gcccgccaga ttggcaccga agactttgct
    51241 cgcacgatgc cggaatattg tggtgtgatc tccaaaagcc cgacggtgaa agcagttaaa
    51301 tcgaagattg aagcggaaga agagaagttc gacttcagca ttctcgataa agtggttgag
    51361 gaagcgaata acgttgatat ccgcgaaatc gcccagcaga ccgagcagga agtggtggaa
    51421 gtggaaaccg tcaatggctt cggcccgaac gacgtgatcc tcgatatccg ttctatcgat
    51481 gaacaggaag ataagccact gaaagtcgaa gggattgatg tggtttctct gccgttctat
    51541 aaactgagca ccaaatttgg cgatctcgac cagaacaaaa cctggctgct gtggtgtgag
    51601 cgcggggtga tgagccgtct gcaggcgctc tatctgcgcg agcagggctt taacaatgtg
    51661 aaggtatatc gcccgtaatt tgtggttttt acgtcgcatc tggtcagatg cgacgtttgc
    51721 cgcatccgac actactcgta ataattataa atccctgccg ccatcaccag ttgtgatgcc
    51781 acttcatggg ctttttcacg cccaaccaac aggtcgataa ttttcagacc aaagtcgata
    51841 gctgtacccg gcccctggct ggtcagcaat tttacccgtg catcccagac gacgcgcttg
    51901 tccagccatt gttcggcggg aattttgtct ttcagcgtcg ggaagccggt catattacca
    51961 atcgggaaga tatcgtgcgg caccagcacg gtggctggcg cggcgcaaat agccgcgacg
    52021 atacgcccgg aacggtggaa ctgtttaacg gtttcaacca gcagagtgct atcgcgaaaa
    52081 cactccgcgc ctttaatgcc accaggcagc acgatcacgt catattcgcc atcagccact
    52141 tcgaccagcg gcgcatccgc cagcagcttc acgccgcgcg agcaggtaat cgccaggtta
    52201 ccatcgctgg cgacgctggc agtggtgact ttgataccgc cgcgaaccag cagatcgata
    52261 gtggtgacgg cttcagtctc ttcactacca ggggcgaggc aaaccagtgc cgatgcgctc
    52321 atattcactc tcctttcttt ttaccatttc aaacaggcgg gtgttttccg gtacggcaat
    52381 cccatgcgcg cgggcgcggc gtaagagaaa accattgata tagtcgattt cggtgtggcg
    52441 cagcgcgcgg atatcctgca acatcgacga gatattttcc gctgtggcat caatcacctg
    52501 catcacgtaa tcacgcaaat cttccgctga agtatgatgc ccttcgcgct cgatcactgc
    52561 cgcgacttct tcgcatattt gcataatttc ttgcggatga tgacgtaatt caccgttcgg
    52621 gcaattccag atggcagtca gtggattaat cacgcagttg actgccagct tgcgccacag
    52681 ctcggcgcga atattgttat gccaggcaac gtcaggcaac acggtttgca aaatatccgc
    52741 cagataactg taatccccgt cctgttgccg tgccgggcca atatgcgtga taccgtttgc
    52801 cacatgaata atgacattgc cgtcgcggcg ggctgcatgg gtggtggtgc ccatcagtaa
    52861 tggctgctga atgttttgca actcttcgat ggtgcccatg ccgttgtgaa ttaacagtat
    52921 tggcgtagtt acaggcagtg tggacgcgag gcttttgacg gcatcggaaa cctgccatgc
    52981 tttcagcgtc accaggagca gatcgctggt ggcgagaaaa tcgggatcgt tggcggtcag
    53041 cgattcgtta aatatcgaac catctgtctc aaccagattc acgctacaat aaggttgcgg
    53101 tacgcgcagc cagccctgaa cttcatgacc ctgtttgcaa agtgctgtaa gccataattg
    53161 ccctaaggca ccgcatccca atacggtaat tttcattgtt cctcctcacc cgcaaccact
    53221 ccgggtgttc aataaggcta tcccttaatt gtgcatgctg ttgcgactat gcacaattaa
    53281 gggatacgtc ctggtgcagg actgtcggtt atttaacttt gcgggtatta tgcttcgcat
    53341 caaaaatgaa gggagaggaa aagatgccat ctttcgatat tgtctctgaa gttgatcttc
    53401 aggaagcacg taacgcggtc gataacgcga gccgcgaagt ggagtcccgt tttgacttcc
    53461 gtaacgttga agcctcattt gagctgaacg acgccagcaa aaccatcaaa gtgttgagcg
    53521 agtccgattt ccaggtcaat cagttgctgg atattctgcg tgccaagctg ctgaagcgcg
    53581 gcattgaagg cagttcgctg gatgtaccgg agaatatcgt tcatagcggt aaaacctggt
    53641 ttgtggaagc gaaactgaaa cagggcattg agagcgcgac ccagaagaaa atcgtcaaga
    53701 tgatcaaaga cagcaaactg aaagtgcagg cgcaaattca gggcgatgaa atccgcgtaa
    53761 cgggcaaatc tcgtgatgat ttgcaggctg tcatggcgat ggtacgtggt ggcgatctcg
    53821 gtcagccgtt ccagttcaaa aacttccgcg attaatcgcg actatgtgcg ttttgttcat
    53881 gtcagatgcg gcatgaacgc ctgattcggc ctacaaaggt ttgtaattca gaaactttgt
    53941 aggcctgata agcgtagcgc agcaggcaat tcgccgcgtt ttttatgcct gacgaattgc
    54001 ctgttctatc tcaaagcgat tcgtcacttt gctgtcgatt ttcacataag ctgaatgttc
    54061 ttcttctgca atcaacactt ctttgatgcc ttcagtttct agcaaacgca cttttaacgc
    54121 ctcgtttgcg gcaatgttcg ccgggatttc aatgcgcaaa ctgctgacat acggcggttc
    54181 tttcatggta ctggcgactg tcagccacac tgcggccagc attgcgccag cgagaaatac
    54241 cccctgaccg tcaaacatgc cgttaatcca gccgcccagc gaaccgccaa tcgccacgcc
    54301 aagaaactgg ctggtggagt aaacacccat cgccgtacct ttgtaacctg ctggcgactc
    54361 tttactgata agtgagggca ggagggcttc catcaaatta aacgccacaa agaaaagctg
    54421 cacgccgacc accagttgcc agaactgcgt ttgcgcgttc cacaacacaa tttccgcaac
    54481 cacgatcaac ccgacgcaga agacaaagac ttgcttcatt ttgcgcttaa cttcagcgta
    54541 gataatgaaa ggcacgaccg agccaaaggc gattagcatt gtcgccagat agaccttcca
    54601 gtgttcagcc gccgggaacc ctgcatcagc cagttgtccg ggcagggcaa caaacgtcga
    54661 catcagcaaa atatgcagac acataatgcc aaagttgagt ttcagcagtc gcggttccgc
    54721 cagcacttta ctgaaactgc ctttcaccat tccggactca cgattaagta cgtgagtgct
    54781 actgttgggc acaacccaaa tggtcaacgc aatgccggtc gttgccagaa tagcgatcat
    54841 ccagaacagc gcgtgcagcc caagtttgtg agtgatgatc gggccaagca ccatcgcaat
    54901 ggcaaaggta atgccaaagc tcacgccgat aaacgccatc gctttggtgc ggttttgttc
    54961 gcgcgtgaga tcggaaagca gcgccataac ggcggcggca attgctccag agccttgtag
    55021 cgcccggccc agaataattc cccagatgga gtcggaaagc gcagcgataa cgctaccggc
    55081 ggcaaacacc gccagcccac cgacaattaa tggtttgcga ccaatgcggt cggaaagcag
    55141 gccaaacgga atctgaaaaa cggcctgagt cagaccataa ataccaatgg caataccgat
    55201 taatgcttcg ctggcacctt gcagagccat gccgtacgtg gtcagaaccg gcagaaccat
    55261 aaacatgccc agcatgcgca acgagaatac ggtccctaaa ccccaggtcg cgcgcctctc
    55321 acctggcgtc attttataat cgttcattac cacctctgtt ttaaattcgc gactagtgta
    55381 aagcggcaaa cgaatggggt aaacatgcgg attttttagt aaatatttcg agagggatat
    55441 gtttctaatg ctaagaaaaa aggtgccgta gcaccttttt aatagagagg ttttgttacc
    55501 acacagcagc cagcagcgta tgcgagtccg gtaccataaa atcaacggac atcatcaccg
    55561 agagggcagt gatggcgatg atagagaagc cgaacagctt gcgcgcccag attctgtcat
    55621 cagcaacttt ataaccgcgc agagccatac ctaaccacca gacgctaacc gccgcggcga
    55681 ccaccagata tttataccca gcgtaaccgc caagagagag catcagcgtg gcaacggcaa
    55741 aggcgatgat atacagcgtg atgtgattct tcgccaccga aatgcctttt accactggca
    55801 ataccggaat gtttgccgcc tggtaatcct taaagcggaa aatggcgatg gcataggagt
    55861 gaggcatctg ccacaggctg aagatagcca gcaggatcgc tgcgccgcta tcgaactcac
    55921 cggttaccgc acagtagccg atcaccggcg gcgcagcgcc ggagagcgaa ccaatcaacg
    55981 tgccgtagac agagtggcgt ttcatgtaca ggctataaac gccgacataa accacaaagc
    56041 ccatcacccc cagccagcag gccagcggat tcgcgccaaa ccacagcagc ataaagccag
    56101 caatacccag caacgtggcg tacaccagcg agacagcagg agagatcagg cctttcacca
    56161 gcacccgatt cttcgtcctt tccatctttc tgtcgatatc cctgtcgatg tagttgttaa
    56221 acacacaacc cgacgccaca accagtgaca ccccaaccag cgtgtagata aacaggggat
    56281 aatcaatgct gccctttgag gccagcagga atcccccaat caccgagatc aggttgccaa
    56341 agatgatgcc tggtttcgtt acttgcaggt attgcttaaa catcataacc gccgctctta
    56401 gtgcatcatc atgttgtagt tgaggttcca cataatccag atggagccta caaccaggat
    56461 agcgatgatt agcacggtga agacaaacgc cgtcatgttc cagccttcat ctgatttggt
    56521 attcatgtgc aggaagcaca ccagatgcac cagaacctgt accactgcca ttgccaggat
    56581 tgttcccaga attacggccg gagaggcagc tcctgtcatc accatccaga acggaatcac
    56641 cgtcagaatg atcgacagga taaagcctgt catgtaggtt tttacgctgc catgggacgc
    56701 gccgctgtga tcggtagaat gactcattac atcgccccca tcagataaac aacagtgaac
    56761 acacagatcc aaaccacatc caggaagtgc cagaacaggc tcaggcacat gatgcgggta
    56821 cggttagtgc tggtcaggcc gcgacgggcg atttgcacca tcagcaccgc catccagata
    56881 agaccagaag tgacgtgcag accgtgcgtg ccgaccagcg caaagaacgc tgacaggaag
    56941 ccgctgcgat ccggacccat gccgttaaca atcaggtgat ggaattcata gatttccatc
    57001 ccgataaatc cggcaccaaa caaccaggtc aacgccagcc aggagataac ctggcttttg
    57061 ttgtttttgt acatggcgat agccgccatg ccgtaggtga tggagctgaa caacagcaag
    57121 aaagtttcaa ccagaacgaa cggcagttcg aaaatgtcct tacctgtcgg gccgcctgcg
    57181 gtgccgttca ccagaacggc ataggtagca aacaagatag agaacagaat gcagtcgctc
    57241 atcaggtaga tccaaaatcc gaagattttg gttccgcctg catcgtggtg cccgtgttcg
    57301 tgcgcgtggg cagtcgcgtg cgtcaaagta tcagttgcca tttttcagcc ctgccttagt
    57361 aatctcatcg aaatgctggt tttccagttt ttcgatttct gccaccggca cgtagtaatc
    57421 cacgtcctcg tcgaagcttt tcacgatcca ggtgatgatc atgcctgcga agccaacaat
    57481 cgccagccac cagatatgcc agatcatggc gaaaccgaag atggtggaga aagctgcaat
    57541 gacgatacct gcaccgctgt ttttcggcat atgaatttct tcatagtggt caggcttttt
    57601 atacgcttcg cctttctctt tcatttccca gaatgcatca cgttcgtgaa cgtgcggcac
    57661 tacggcaaag ttatagaacg gaggcgggga agaggttgcc cactccagcg tacggccacc
    57721 ccacgggtcg ccagtcaggt cacggttctg gtcgcggtcg cgaatagaaa cgtacatctg
    57781 aataacgagg cagagaatac ccagcgcaat cagtactgca ccgctggctg caatcatcag
    57841 catggtgtgg aactgcgggt caatctgctg gctcaaacga cgggtcatgc ccatgaagcc
    57901 cagcgcatac agtggcataa aggcaacgaa gaagccgatg atccagaacc agaacgcgcg
    57961 tttaccccag gtttcgttca gtttgaaacc gaacgcttta ggccaccagt aggtcatccc
    58021 tgcgaagcag ccgaagacca cgccgccgat gatcacgtta tggaagtgcg caatcaggaa
    58081 caggctgtta tgcagaacga agtccgcgcc cggtacggcc agcagcacgc cagtcatccc
    58141 gcccaccgag aaggtgacga taaaaccgat ggtccacagc atcgcagaat ggaacacgat
    58201 gcggccctga tacatggtga acagccagtt gaagatcttc accccggtcg ggatggcgat
    58261 aatcattgtg gtgataccaa agaaggcgtt tacgttcgcg cccgcaccca tcgtaaagaa
    58321 gtggtgcagc caaacgatga acgacagcac ggtgatacag acggttgccc ataccagcga
    58381 ggtataacca aacagacgtt tacgcgagaa ggttgccgca atttcggaga acacaccgaa
    58441 aacaggcagg atcaggatgt aaacttccgg gtggccccag gcccaaatca ggttgatgta
    58501 catcatcatg ttgccaccca tatcgttggt aaagaaatgg gtgcccagat agcgatccag
    58561 ggtcaacaac gcgacggtaa ccgtcagaat tgggaaggaa gcaataatca gtacgttcgc
    58621 gcacagtgat gcccaggtaa atactggcat cttgaacatg gtcatgcccg gtgcgcgcat
    58681 cttcagaatg gtaacgaaga agttgatacc ggtaagcgtc gtacctatac cggatagctg
    58741 gagactccat atccagtaat cgacaccgac tcccggactg tactctattc ccgatagcgg
    58801 tggataggcc agccagccgg tctgcgcaaa ttcgcccacg ccgagagaaa cgttaaccag
    58861 aatcacacca acaacggtaa accagaagct taagttgttg aggaacggga acgcaacgtc
    58921 acgcgcgccg atctgcagcg gaaccaccag gttcatcaga ccgataacga aaggcatcgc
    58981 cacgaagaag atcataatca cgccgtgcgc ggtaaagatc tgatcgtagt ggtgaggtgg
    59041 caggaagccc gcttcgcccg ccgaggcaag agcctgctgg ctacgcatca taatggcgtc
    59101 agcaaaacca cgcagcaaca tcacaatcgc cacgatgata tacatgatac cgaggcgttt
    59161 atggtcgacg gaggtcagcc actctttcca caggtaggtc cacttaccga agtaagtgat
    59221 caggccaacg agcgccagac ctcccaaaat aatgccagcg atcgtaacca tgacgatagg
    59281 ttcatggaac gggactgcat caagtgataa ttttccgaac atctttattc ttcctcaacc
    59341 cctttaatgg gcggattccg cgtggctcat gtccatgcct tccatacctt cgtgtgcgct
    59401 gtgctcacct tctggctggg tcatgtccat gctcttaccg tgagccataa acttgttaat
    59461 tacatcggca aacaagtctg gtttcacgtt ggagaaatat tccacctggt tgtattcgct
    59521 aggcgcggcc agtttttcga acgcagccat gtcagacatg gtgttcggcg actgcttcgc
    59581 ttttgcgacc cactggtcga atgcggcgcg atccggtgtt gcaatagctt tgaacttcat
    59641 gcctgagaag cccgggccgc tgtagctggc ggagataccg tcataagtgc cgggttcgtt
    59701 ggcgatcaga tgcaggcgag tctgcatacc ggccatggca taaatctggc tacccagacg
    59761 cggaatgaag aaggagttca tcacggagtt ggaggtcact ttgaagtaca ccggagtgtt
    59821 cgccgggaaa gcgatttcat tcacggtagc aatgccctgt tccgggtaga tgaagaacca
    59881 tttccagtcc atggaaacca cttcgatggt aatgggcttc tcgtcgtgtg ccagcggctt
    59941 gctaggctca agagcgtgag tggttttcca ggtcagtact gcaaggaaga tgatgattaa
    60001 gataggtacc gtccagacca cagcttccac tttattggag tgtgaccagt tcgggctgta
    60061 cttagcatct ttattgctcg cacggtactt ccaggcgaaa ccaacagcca tcaagattgc
    60121 gggaataacg acaatcaaca tcaggccaaa tgccgtcagt atcagtgaac gttgctccag
    60181 accaatctgt cctttgggat ctaacagcgc agaattacag ccactgagca atacagtgcc
    60241 tgcaaataat gacaaccatc ccaaactttt attgtatttc ctgagtctca tttaacgacc
    60301 tcaattccac gggatctggt ggcgtttaaa gtgtgtgggc attttacggg aaggttacat
    60361 tactgtaaac atgattaaat tcctgttact tggatttggc tgccaggtca catatgttgc
    60421 aaaacatatc aggttgattt taagaagctg ttgcaaaagg gcgttataac aaaaggggag
    60481 tgctgaagga gtctgggcgg gcaattggta taaccaatgt gaaataaaac aattatttaa
    60541 caaataatta tcattagagt gacaactggg tgatgagaaa ataaagactc attagctgaa
    60601 tcgtgtaaga aaaatagaat ttataaatgg cgccaataat ttccaaacgc taagccgcac
    60661 aaaagaacaa atattaataa gcgatactga tgtttattta gccgtaataa ttacggcgag
    60721 tgattactac agctaaataa tatttacaga ttacgtcaga tgcgtttttc gtagcgccag
    60781 ataatccagc aaaccaccaa gcacgacgcc agaaagggcg actaaaaccc cgacttccag
    60841 cagagcaggc aggaaagaga agtgcgtcaa atccagcgcg tccatcgtca atagcagtaa
    60901 ccacacggcc aacaggctga cgcccgccgc cagtgtccac atggcaaagg cataacctgc
    60961 cggatatgcg gtacgggaga taaagttgtc atttactcgt gtatattcaa gcgtctggcg
    61021 gcaaaccagc agcaaaataa gccctggtac ggcagcggcg acggagaata gatagaatgt
    61081 cgaccagccg tgtgcttcaa caaaccaacc cgccacgggg ccgacataaa ctcgccctac
    61141 agcagaaagc gctgagagca gggcaaattg agtagcggaa aatgacttat tacatagcgt
    61201 cattaacagc gcgacaaagg ctgatgtgcc catcccgcca cagaggtttt cgaaaaagac
    61261 ggctgcgccc atgctgtaga gatgcttatc agtaatcgac agcagccagt aaccggcgtt
    61321 agacgcacct tgtaaaatgc cgaaaatcag cagtgcccgg aacagtgaca ggcgctgcat
    61381 caaaatccca ccgtacaatg cgccaacaat ggtcgctagt aagccaagcg ttttgttaac
    61441 cacgcctact tcacccgcat caaacccgac gccgcgaatc aaaaacgtgg ttgtcaggct
    61501 catggcgaat gcgtcgccca gcttatacag cacgataaga agcaaaataa gccaggcatt
    61561 attgcgacca aagaaatctc gcagaggtgc aacaaccgct tgttccagcg tttttggcac
    61621 aggaatggtg tcggtcggtt ctggtgcaag caacgtcgcg ataatacagg ggatcaacag
    61681 cgccgccatc aaccagtaca tgccctgcca gcccagccat ttatctgcca gccacagggc
    61741 taggccgccg gaaaccagca tccctaaacg gtaacccaga acgctgattg ccgcgcccgc
    61801 accacgttct tctgccggga gcacatcggt tttccacgca tcgaagacaa tatcctggga
    61861 ggcagagcaa aaagcgatca ccaccgccag cgccgccatc cagcggagtt gggtgcctgg
    61921 ttcgagaaaa cccatcgccg caatggcgac taataacagg atttgcgtgg cgagcagcca
    61981 accgcgtcgc cgcccaaaaa atggaggcgt gtagcggtcc atcagcggtg accagaggaa
    62041 tttaaaaacg taagcctggc ctaccagaga gaagaaacca atggttttga gatcaatatt
    62101 ctcgaccgtc atccaggcct gtaaggtacc ggatgtcagg gcgagcggta gcccggaagc
    62161 aaaacccagg atcagcaata tggctgaacg cggctgttga aaaatacgta aatattgact
    62221 ggacatgggc gtctacaggc ccggcttgca ccgggccaga gggcagaaat taacgcgcgt
    62281 tctgcttgat gaattcgtgg atgctggtgt cctgagacat atcagcgatg gtatcggtca
    62341 gcacactgtt aaccgcatcg gcgatatttt tgttggaggc ctggaacgca ccttcaacgt
    62401 tgtagctggc acgatagttt ttggtcattt tgttgccatt ctgcgcggta gcgatgatgg
    62461 cgatatccgc tttggtcgcg atgttgtagc gcacgttgcc ctgggacacg tcagcataca
    62521 gttggctaac gatgatttgc agattaaccg ggccattcgg accaaccatg taaccacgcg
    62581 cggtcatctg tttttccagc acttcttgca gcaggaaacg cagatcgcgg gaggcggtca
    62641 gggtaacgat ttgattatcg cgggtgactt ttgccagcgc ctgatcggta cgctgatcgg
    62701 caccattaat gcttacggtg acgcccatca ggcttggatc ctgctgtggc agtgtaatcg
    62761 tcggggaaac ttcaatagtt gttggcggtt ttgcgcatcc tgcaagcata aacagagcaa
    62821 ctaacgggaa gaggattttt ttgaacatgt tcgggctctc agagactctt aagcgtgttt
    62881 ggtaaaaatt cccgccatca taacattgcc aacggcgagg ggaagtgggt aaggcatgta
    62941 aattcatcat gttgacgaaa taatcgcccc tggtaaaaga aacactgatg cgaggcctgt
    63001 gtttcaatct ttaaatcagt aaacttcata cgcttgacgg aaaaaccagg acgaaaccta
    63061 aatatttgtt gttaagctgc aatggaaacg gtaaaagcgg ctagtattta aagggatgga
    63121 tgacatctca gcgttgtcgg aggagatatt tcatgatgat acgtgagcgg atagaagaaa
    63181 aattaagggc ggcgttccaa cccgtattcc tcgaagtagt ggatgaaagc tatcgtcaca
    63241 atgtcccagc cggctctgaa agccatttta aagttgtgct ggtcagcgat cgttttacgg
    63301 gtgaacgttt tctgaatcgt catcgaatga tttacagtac tttagcggag gaactctcta
    63361 ctaccgttca tgcgctggct ctgcatactt acactattaa ggagtgggaa gggttgcagg
    63421 acaccgtctt tgcctctcct ccctgtcgtg gagcaggaag catcgcgtaa aaacgcattt
    63481 gcaactgtcg gcgcttttcc agtatgttgc taaagatttt atgaaaaacg gcctgcgggc
    63541 cgttttgttt tgtctggatt ttgcgctttt tgcccagcat tcagacgaaa attgcccggg
    63601 aattgtgaaa aaatacgcga cagcgcgcaa taaccgttct cgactcataa aagtgatgcc
    63661 gctataatgc cgcgtcctat ttgaatgctt tcgggatgat tctggtaaca gggaatgtga
    63721 ttgattataa gaacatcccg gttccgcgaa gccaacaacc tgtgcttgcg gggtaagagt
    63781 tgaccgagca ctgtgatttt ttgaggtaac aagatgcaag tttcagttga aaccactcaa
    63841 ggccttggcc gccgtgtaac gattactatc gctgctgaca gcatcgagac cgctgttaaa
    63901 agcgagctgg tcaacgttgc gaaaaaagta cgtattgacg gcttccgcaa aggcaaagtg
    63961 ccaatgaata tcgttgctca gcgttatggc gcgtctgtac gccaggacgt tctgggtgac
    64021 ctgatgagcc gtaacttcat tgacgccatc attaaagaaa aaatcaatcc ggctggcgca
    64081 ccgacttatg ttccgggcga atacaagctg ggtgaagact tcacttactc tgtagagttt
    64141 gaagtttatc cggaagttga actgcagggt ctggaagcga tcgaagttga aaaaccgatc
    64201 gttgaagtga ccgacgctga cgttgacggc atgctggata ctctgcgtaa acagcaggcg
    64261 acctggaaag aaaaagacgg cgctgttgaa gcagaagacc gcgtaaccat cgacttcacc
    64321 ggttctgtag acggcgaaga gttcgaaggc ggtaaagcgt ctgatttcgt actggcgatg
    64381 ggccagggtc gtatgatccc gggctttgaa gacggtatca aaggccacaa agctggcgaa
    64441 gagttcacca tcgacgtgac cttcccggaa gaataccacg cagaaaacct gaaaggtaaa
    64501 gcagcgaaat tcgctatcaa cctgaagaaa gttgaagagc gtgaactgcc ggaactgact
    64561 gcagaattca tcaaacgttt cggcgttgaa gatggttccg tagaaggtct gcgcgctgaa
    64621 gtgcgtaaaa acatggagcg cgagctgaag agcgccatcc gtaaccgcgt taagtctcag
    64681 gcgatcgaag gtctggtaaa agctaacgac atcgacgtac cggctgcgct gatcgacagc
    64741 gaaatcgacg ttctgcgtcg ccaggctgca cagcgtttcg gtggcaacga aaaacaagct
    64801 ctggaactgc cgcgcgaact gttcgaagaa caggctaaac gccgcgtagt tgttggcctg
    64861 ctgctgggcg aagttatccg caccaacgag ctgaaagctg acgaagagcg cgtgaaaggc
    64921 ctgatcgaag agatggcttc tgcgtacgaa gatccgaaag aagttatcga gttctacagc
    64981 aaaaacaaag aactgatgga caacatgcgc aatgttgctc tggaagaaca ggctgttgaa
    65041 gctgtactgg cgaaagcgaa agtgactgaa aaagaaacca ctttcaacga gctgatgaac
    65101 cagcaggcgt aatttacgca gcataacgcg ctaaattcgc acaaaggccc gtcaccgcca
    65161 ggtggtgggc ttttttttgt catgaatttt gcatggaacc gtgcgaaaag cctctttcgg
    65221 tgttagcgta acaacaaaag attgttatgc ttgaaatatg gtgatgccgt acccataaca
    65281 cagggactag ctgataatcc gtccataagg ttacaatcgg tacagcaggt tttttcaatt
    65341 ttatccagga gacggaaatg tcatacagcg gcgaacgaga taactttgca ccccatatgg
    65401 cgctggtgcc gatggtcatt gaacagacct cacgcggtga gcgctctttt gatatctatt
    65461 ctcgtctact taaggaacgc gtcatttttc tgactggcca ggttgaagac cacatggcta
    65521 acctgattgt ggcgcagatg ctgttcctgg aagcggaaaa cccagaaaaa gatatctatc
    65581 tgtacattaa ctccccaggc ggggtgatca ctgccgggat gtctatctat gacaccatgc
    65641 agtttatcaa gcctgatgtc agcaccatct gtatgggcca ggcggcctcg atgggcgctt
    65701 tcttgctgac cgcaggggca aaaggtaaac gtttttgcct gccgaattcg cgcgtgatga
    65761 ttcaccaacc gttgggcggc taccagggcc aggcgaccga tatcgaaatt catgcccgtg
    65821 aaattctgaa agttaaaggg cgcatgaatg aacttatggc gcttcatacg ggtcaatcat
    65881 tagaacagat tgaacgtgat accgagcgcg atcgcttcct ttccgcccct gaagcggtgg
    65941 aatacggtct ggtcgattcg attctgaccc atcgtaattg atgccagagg cgcaactgtg
    66001 ccgctatact tatccagggc ggcacaacgc tgtaagcggc ttgcgcctga gaatggcatt
    66061 tgcgtcgtcg tgtgcggcac aaagaacaaa gaagaggttt tgacccatga cagataaacg
    66121 caaagatggc tcaggcaaat tgctgtattg ctctttttgc ggcaaaagcc agcatgaagt
    66181 gcgcaagctg attgccggtc catccgtgta tatctgcgac gaatgtgttg atttatgtaa
    66241 cgacatcatt cgcgaagaga ttaaagaggt tgcaccgcat cgtgaacgca gtgcgctacc
    66301 gacgccgcat gaaattcgca accacctgga cgattacgtt atcggccagg aacaggcgaa
    66361 aaaagtgctg gcggtcgcgg tatacaacca ttacaaacgt ctgcgcaacg gcgataccag
    66421 caatggtgtc gagttgggca aaagtaacat tctgctgatc ggtccgaccg gttccggtaa
    66481 aacgctgctg gctgaaacgc tggcgcgcct gctggacgtc ccgttcacca tggccgacgc
    66541 aaccacgctg accgaagccg gttatgtggg cgaagacgtt gaaaacatca ttcagaagct
    66601 gttgcaaaag tgcgattacg acgtacagaa agcacagcgt ggtattgtct acatcgatga
    66661 aatcgacaag atttctcgta agtcagacaa cccgtccatt acccgagacg tttccggtga
    66721 aggcgtacag caggcactgt tgaaactgat cgaaggtacg gtagctgctg ttccaccgca
    66781 aggtgggcgt aaacatccgc agcaggaatt cttgcaggtt gatacctcta agatcctgtt
    66841 tatttgtggc ggtgcgtttg ccggtctgga taaagtgatt tcccaccgtg tagaaaccgg
    66901 ctccggcatt ggttttggcg cgacggtaaa agcgaagtcc gacaaagcaa gcgaaggcga
    66961 gctgctggcg caggttgaac cggaagatct gatcaagttt ggtcttatcc ctgagtttat
    67021 tggtcgtctg ccggttgtcg caacgttgaa tgaactgagc gaagaagctc tgattcagat
    67081 cctcaaagag ccgaaaaacg ccctgaccaa gcagtatcag gcgctgttta atctggaagg
    67141 cgtggatctg gaattccgtg acgaggcgct ggatgctatc gctaagaaag cgatggcgcg
    67201 taaaaccggt gcccgtggcc tgcgttccat cgtagaagcc gcactgctcg ataccatgta
    67261 cgatctgccg tccatggaag acgtcgaaaa agtggttatc gacgagtcgg taattgatgg
    67321 tcaaagcaaa ccgttgctga tttatggcaa gccggaagcg caacaggcat ctggtgaata
    67381 attaaccatt cccatacaat tagttaacca aaaagggggg attttatctc ccctttaatt
    67441 tttcctctat tctcggcgtt gaatgtgggg gaaacatccc catatactga cgtacatgtt
    67501 aatagatggc gtgaagcaca gtcgtgtcat ctgattacct ggcggaaatt aaactaagag
    67561 agagctctat gaatcctgag cgttctgaac gcattgaaat ccccgtattg ccgctgcgcg
    67621 atgtggtggt ttatccgcac atggtcatcc ccttatttgt cgggcgggaa aaatctatcc
    67681 gttgtctgga agcggcgatg gaccatgata aaaaaattat gctggtcgcg cagaaagaag
    67741 cttcaacgga tgagccgggt gtaaacgatc ttttcaccgt cgggaccgtg gcctctatat
    67801 tgcagatgct gaaactgcct gacggcaccg tcaaagtgct ggtcgagggg ttacagcgcg
    67861 cgcgtatttc tgcgctctct gacaatggcg aacacttttc tgcgaaggcg gagtatctgg
    67921 agtcgccgac cattgatgag cgggaacagg aagtgctggt gcgtactgca atcagccagt
    67981 tcgaaggcta catcaagctg aacaaaaaaa tcccaccaga agtgctgacg tcgttgaata
    68041 gcatcgacga tccggcgcgt ctggcggata ccattgctgc acatatgccg ctgaaactgg
    68101 ctgacaaaca gtccgttctg gagatgtccg acgttaacga acgtctggaa tatctgatgg
    68161 caatgatgga atcggaaatc gatctgctgc aggttgagaa acgcattcgc aaccgcgtta
    68221 aaaagcagat ggagaaatcc cagcgtgagt actatctgaa cgagcaaatg aaagctattc
    68281 agaaagaact cggtgaaatg gacgacgcgc cggacgaaaa cgaagccctg aagcgcaaaa
    68341 tcgacgcggc gaagatgccg aaagaggcaa aagagaaagc ggaagcagag ttgcagaagc
    68401 tgaaaatgat gtctccgatg tcggcagaag cgaccgtagt gcgtggttat atcgactgga
    68461 tggtacaggt gccgtggaat gcgcgtagca aggtcaaaaa agacctgcgt caggcgcagg
    68521 aaatccttga taccgaccat tatggtctgg agcgcgtgaa agatcgaatc cttgagtacc
    68581 ttgcggttca aagccgtgtc aacaaaatca agggaccgat cctctgcctg gtagggccgc
    68641 cgggggtagg taaaacctct cttggtcagt ctattgccaa agccaccggg cgtaaatatg
    68701 tccgtatggc gctgggcggc gtgcgtgatg aagcggaaat ccgtggtcac cgccgtactt
    68761 acatcggttc tatgccgggt aaactgatcc agaaaatggc gaaagtgggc gtgaaaaatc
    68821 cgctgttcct gctcgatgag atcgacaaaa tgtcttctga catgcgtggc gatccggcct
    68881 ccgcactgct tgaagtgctg gatccagagc agaacgtagc gttcagcgac cactacctgg
    68941 aagtggatta cgatctcagc gacgtgatgt ttgtcgcgac gtcgaactcc atgaacattc
    69001 cggcaccgct gctggatcgt atggaagtga ttcgcctctc cggttatacc gaagatgaaa
    69061 aactgaacat cgccaaacgt cacctgctgc cgaagcagat tgaacgtaat gcactgaaaa
    69121 aaggtgagct gaccgtcgac gatagcgcca ttatcggcat tattcgttac tacacccgtg
    69181 aggcgggcgt gcgtggtctg gagcgtgaaa tctccaaact gtgtcgcaaa gcggttaagc
    69241 agttactgct cgataagtca ttaaaacata tcgaaattaa cggcgataac ctgcatgact
    69301 atctcggtgt tcagcgtttc gactatggtc gcgcggataa cgaaaaccgt gtcggtcagg
    69361 taaccggtct ggcgtggacg gaagtgggcg gtgacttgct gaccattgaa accgcatgtg
    69421 ttccgggtaa aggcaaactg acctataccg gttcgctcgg cgaagtgatg caggagtcca
    69481 ttcaggcggc gttaacggtg gttcgtgcgc gtgcggaaaa actggggatc aaccctgatt
    69541 tttacgaaaa acgcgacatc cacgtccacg taccggaagg tgcgacgccg aaagatggtc
    69601 cgagtgccgg tattgctatg tgcaccgcgc tggtttcttg cctgaccggt aacccggttc
    69661 gtgccgatgt ggcaatgacc ggtgagatca ctctgcgtgg tcaggtactg ccgatcggtg
    69721 gtttgaaaga aaaactcctg gcagcgcatc gcggcgggat taaaacagtg ttaattccgt
    69781 tcgaaaataa acgcgatctg gaagagattc ctgacaacgt aattgccgat ctggacattc
    69841 atcctgtgaa gcgcattgag gaagttctga ctctggcgct gcaaaatgaa ccgtctggca
    69901 tgcaggttgt gactgcaaaa tagtgacctc gcgcaaaatg cactaataaa aacagggctg
    69961 gcaggctaat tcgggcttgc cagccttttt ttgtctcgct aagttagatg gcggatcggg
    70021 cttgccctta ttaaggggtg ttgtaagggg atggctggcc tgatataact gctgcgcgtt
    70081 cgtaccttga aggattcaag tgcgatataa attataaaga ggaagagaag agtgaataaa
    70141 tctcaattga tcgacaagat tgctgcaggg gctgatatct ctaaagctgc ggctggccgt
    70201 gcgttagatg ctattattgc ttccgtaact gaatctctga aagaagggga tgatgtagca
    70261 ctggtaggtt ttggtacttt tgccgttaaa gagcgtgctg cccgtactgg ccgcaacccg
    70321 cagaccggta aagagatcac catcgctgct gctaaagtac cgagcttccg tgcaggtaaa
    70381 gcactgaaag acgcggtaaa ctaagcgttg tccccagtgg ggatgtgacg aagttcaagg
    70441 ggcgcatcta ctgatgtgcc ttttttattt gtattcggtg actttctgtg tcttgtgggc
    70501 tgacaattgc ccccgtttct tgtcacaata ggcctttgcg cgcatcgata cgttgcgtga
    70561 ggtacacagt catctacagc ggagtgttgt tacaccatga tggacagctt acgcacggct
    70621 gcaaacagtc tcgtgctcaa gattattttc ggtatcatta tcgtgtcgtt catattgacc
    70681 ggcgtgagtg gttacctgat tggcggaggc aataactacg ccgcaaaagt gaatgaccag
    70741 gaaatcagcc gtgggcaatt cgagaatgcc ttcaacagcg agcgtaatcg catgcagcaa
    70801 cagctgggcg atcaatactc cgagctggca gcgaacgaag gctatatgaa aaccctgcgt
    70861 caacaggtgc tgaatcgtct gatcgacgag gcgctgctgg atcagtacgc acgtgagctg
    70921 aaactgggta tcagcgatga gcaggttaaa caggcgattt tcgcgacccc agccttccag
    70981 gttgacggca aatttgataa cagccgctat aacggtatcc tcaaccagat ggggatgacc
    71041 gccgatcagt acgcccaggc gctgcgtaac cagctcacta cccaacagct gattaacggc
    71101 gttgccggta ccgattttat gctgaaaggt gaaaccgacg agctggcggc actggtcgcg
    71161 caacaacgcg tggtgcgtga ggcgactatc gatgttaacg cgctggcggc gaagcagcct
    71221 gtgaccgaac aggagattgc cagctactac gaacaaaaca aaaacaattt catgacgccg
    71281 gaacaattcc gcgtgagtta catcaagctg gatgccgcaa cgatgcagca accggttagc
    71341 gatgcggata tccagagcta ctacgaccag catcaggatc aattcaccca gccgcagcgt
    71401 acccgctaca gcatcatcca gaccaaaact gaagatgaag cgaaagcggt acttgatgag
    71461 ctgaataaag gcggtgattt tgctgcatta gccaaagaaa aatctgccga tattatctct
    71521 gctcgtaacg gcggcgatat gggttggtta gaagatgcca ctatcccgga cgaactgaaa
    71581 aatgctggtc tgaaagaaaa aggccaactg tctggtgtta tcaaatcttc ggtcggtttc
    71641 ctgattgtac gtctggacga cattcagcca gcgaaagtga aatcgttaga cgaagtacgt
    71701 gacgacattg cggcgaaagt gaaacacgaa aaagccctcg atgcgtacta cgcgctgcag
    71761 cagaaagtga gcgatgcggc aagcaacgac accgagtctc tggccggtgc agagcaagct
    71821 gccggcgtta aagccactca gacgggttgg ttcagcaaag ataacctgcc ggaagagttg
    71881 aacttcaagc cggttgccga cgctatcttt aacggcggtc tggtaggtga aaacggcgcg
    71941 ccgggcatca actctgacat catcaccgta gacggcgacc gcgcattcgt gctgcgcatc
    72001 agcgagcaca aaccggaagc ggtgaaaccg ttggcagatg ttcaggaaca agttaaggca
    72061 ctggttcagc acaacaaagc tgaacaacag gcgaaagtgg atgctgagaa actgctggtt
    72121 gatttgaaag ccggcaaagg tgcggaagct atgcaggctg ccggtctgaa atttggagag
    72181 ccgaaaacct taagccgttc cggtcgtgac ccgattagcc aggcggcgtt tgcactgcca
    72241 ctgccagcga aagacaaacc gagctacggt atggcgaccg atatgcaagg caatgtggtt
    72301 ctgctggcgc tggatgaagt gaaacaaggt tcaatgccgg aagaacagaa aaaagcgatg
    72361 gtgcagggta tcacccagaa caacgcacaa atcgtctttg aagctctgat gagtaacctg
    72421 cgtaaagagg cgaaaatcaa aattggcgat gcgctggaac agcaataatc ctgaagccgc
    72481 ctcgcaaaaa aatgcgttgc agctgtaaca agaaaaggtc gctttcgcgg ccttttccat
    72541 ttctgaacat tgccatttgt ttactgtttt cactgccgtt aaggtgattc cactgttaac
    72601 aaacaaggag aaaacagtat gaaacacgga attaaagcac tgctcattac cctgtccctg
    72661 gcctgtgccg gaatgtctca tagcgcgctg gcggcagctt ctgtggcgaa accgacggcg
    72721 gtagaaacca aagcggaagc tcctgcagca caaagtaaag cagcagtacc ggcgaaagcc
    72781 agtgacgaag aaggcacccg ggtcagcatt aataatgcca gcgcggaaga gctagcccgc
    72841 gcgatgaatg gcgttggcct gaagaaagcg caggcgattg tcagttatcg cgaagagtac
    72901 ggtccgttta aaactgtgga ggatctaaag caggtgccgg ggatgggcaa ttcgctggtg
    72961 gaacgtaatc tggcggtatt aaccctgtaa ttaatttgca tagtggcaat tttttgccag
    73021 actgaagagg tcataccagt tatgacctct gtacttataa caacaacgta aggttattgc
    73081 gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg acgtttacca gcacgtcaac
    73141 aacgcccgct accttgaatt tctcgaagaa gcccgctggg atgggttgga aaatagcgac
    73201 agttttcagt ggatgacggc ccataacatc gccttcgtcg tggtcaatat caatattaac
    73261 tatcgtcgcc cagcggtatt aagtgacctg ttaactatta ccagtcagtt gcagcaatta
    73321 aacggtaaaa gcggcatctt aagccaggtc attacactgg agccggaagg gcaggtggta
    73381 gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc attagctctg
    73441 gaaggggaat tgcgcgaaaa gctggagcag atggttaagt aaacgttttg tggtgccgga
    73501 tgctcaagcc gcatccggcg acacccggaa taattacctc aacccggttt tctgcttcat
    73561 cgctgccatc accgtcggtt tatcggccag ataatgattc aaaccgttgg cgcgtaaatt
    73621 acatgccgca caatgaccgc aaccgtcgcc tttaatgccg ttatagcagg tcaacgtttc
    73681 gttacggact aaatccagtt tgccgtaata atctgccagc gcccaggttt ccgctttatc
    73741 aatccacatc agcggcgttt caaaacgaat atctttcgcc atgcccaaac tgacggcatg
    73801 gtttagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat ccgtttcaca
    73861 gacgccggta attacggctt ctgcttttac ctgatacgca tatattgccg ccagcgtcag
    73921 gaacaaaata ttacgccctg ggacaaacgt attcgggatg ccatcggctt caggttcata
    73981 atcaggcacc ggaatgctgt cacgtgtcag gctgctgacc gccagctcgt tgagcagcgt
    74041 tacatcaaga accttatgcg cgcgtgctcc cagtttcagc gccagttcgc gtgccacgtc
    74101 gatttctgcg cgatgtcgct gaccgtaatc gaacgtcacg caatggactt catcatattg
    74161 ttgtaatgcc tgcaccagac aggtggtgga atcctggcct ccactgaaca caacgacagc
    74221 acgtttcata gataatcctg cttgaacaat aaaagcgtta tggtaacgcc tgcgattaac
    74281 ccggaccagc tattcattgc gacggcgcgg gaagccaggc ttcggtaaat tcaaaccagc
    74341 cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc actaatgcga tagtgatagt
    74401 tgaacagcgg cgtcagcgtc gcgtctgcca ttaactggct aaaaaccgct ttcagggcat
    74461 taaatcggtt ttcctcatca ggcattactt gcaccgcgtc cagcgtcgat tgcagatgtg
    74521 catatgctgg agcgtcgaaa acatgtggcc atagtggatc gcaacgtagc cattgctcca
    74581 gagtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca
    74641 gtagggtcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt
    74701 ccgctgccag tgtcgcctgt aggcgttctg ccatggtatg aagttctatc ggtaagtgat
    74761 aaaccagcgt caaggttttc ggtagtttga cttcatccgg tacttgccag tgcggaatag
    74821 tccagcctgg cagtaatgca tgactggcgg tgatcaggtt ttctccgact tctaacgttt
    74881 gtaataaacc ggattgatga ataatggaga tcacttttcg cgcctgccag agggagagtc
    74941 ggggactttt gcgcaacgtt aaatagcaaa aacctaaact gatgccacta ctgacctggc
    75001 tgacccgttg cagctcctcc ggtttgccga tggtgatttg cacgggatgc cgacaactgg
    75061 ttcccaaatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct ttaagcagcg
    75121 gatgacgcaa atggtaataa tcatggctct ccaggcgcac cagctccgct gtgaattgtg
    75181 ttaagcgaaa aggaccagtg ccgatcagtg ggaattgcgg atgcgccaga tggctgcaat
    75241 agctcgccag ccggtgcgca agccagtaat cggggcgatg taaaaagaag gtcagacact
    75301 gcggatgggt gacctcaata cgcttcacgc taataaataa ttgatccagt gctggcagtt
    75361 gtaacagcat caataatcgc tggtgtaagt gtgaggcttt tactgcatcg ccgttatgcc
    75421 agtgtagggt tgaacgaaga taaaagtccc agcgtaaccc gtcagtagag gtttcccagt
    75481 gatgcgctaa atcgccaatc gggcgctgag tattattatc gaagcgggtc aggccggaaa
    75541 atatctgccc ggcgagatgc tgctcggcac ggccgggcaa aaagcctggt tgtagcggtt
    75601 cgagcgggcg atagtaggga atacgcaacg tgggtgtatc gttttgccat tgtccgccca
    75661 taaacggctg taacagagtg cgcagctcac ctggggccag ttgcgccagc tccagcacat
    75721 cttgctgctt tccggtttcc agtgcctgtt ccatcatcgc attgcgtagc gattccggcg
    75781 tgaccagaaa gcgtaattgt ccgcgctttc cgcgtcctga ctgcgcctgc cactccagcc
    75841 atcccgcctc ctgtgcctga cgcaacagcg tacgaacatg gcgttcgctg caaaaacagc
    75901 gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggcggaaggt tgccacagac
    75961 gttgatactg gttaagacgg ttgagcaatc gcatataaac ccggaacaat attatttaac
    76021 tattcactat tacttccgta tatatcaggt gatactcaat caccattaac cgtgtcacag
    76081 agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt tattgatgcc
    76141 tgaccatcat ttaggtgaga aaaccctctc tactttggcg cgactgcgtg aacgcgacat
    76201 taccctcact tttgccacgg ggcgtcatgc gctggagatg cagcatattc tcggggcgct
    76261 atcgctggat gcgtatttga ttaccggcaa cggaacgcgc gtgcattctc tggaaggtga
    76321 acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt atcagcaatg
    76381 ggatacccga gccagcatgc atatcttcaa tgacgacggt tggtttaccg ggaaagagat
    76441 ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt tatcagataa tcgatgtcaa
    76501 aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg acgatcttac
    76561 acgcttgcag atccagctat acgaagcatt aggcgagcgt gcacatttgt gtttttccgc
    76621 cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa ggcgctgcat tgacggtgct
    76681 gacccaacat ttaggtttat cgttgcgcga ttgcatggcc tttggtgatg cgatgaacga
    76741 tcgcgaaatg ttaggcagcg tcggtagcgg atttattatg ggcaatgcga tgccgcaact
    76801 gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaatcagg ctgtctctca
    76861 ctatttgacg cactggctgg actatccaca tctaccttat tcccccgaat aacgagatcc
    76921 cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt ttgtgccgtg
    76981 gtgtttaaac cgcacagaat aaattgtcgt gatttcacct ttaaaataaa attaaaagag
    77041 aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct gctggcctta
    77101 ctgcagcagg attgcaccct ctctttgcag gcactggctg aagccgttaa tctgacaacc
    77161 accccttgct ggaagcgcct gaaacggctg gaggacgacg gtatccttat cggcaaagtc
    77221 gccctgctgg atccggaaaa aataggcctc ggcctgaccg cttttgtgct gataaaaacg
    77281 caacatcaca gcagcgaatg gtattgccgc tttgtcacgg tggttaccga aatgccagaa
    77341 gtgctggggt tctggcgcat ggctggtgaa tacgattatc tgatgcgcgt ccaggttgcc
    77401 gacatgaaac gctacgacga gttttataag cgtctggtaa acagcgtgcc ggggctgtcg
    77461 gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt acccatcgaa
    77521 taaatatcca gaatcaagtc aggacacaac gcgtgcgatt atttgctcaa ttaagctggt
    77581 atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc attatcgcga
    77641 tgctgcaact ggttccgcca aaagtggttg gtattgttgt cgatggcgtg acagaacaac
    77701 actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt gccgttgtgg
    77761 tttatctcct gcgttacgtc tggcgcgtat tgctgtttgg tgcgtcttat caactggctg
    77821 ttgaactgcg tgaagattat taccgtcagc taagccggca gcatcctgag ttttacctgc
    77881 gtcatcgcac gggtgatctc atggctcgtg cgaccaatga cgtcgatcgc gtcgtgtttg
    77941 ccgccggaga aggggtgctg acgctggtgg attcactggt gatgggctgc gctgtgttga
    78001 ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgttg ccgatgccag
    78061 tgatggcgat catgatcaag cgcaacggcg atgccttgca tgaacgcttt aagctggcac
    78121 aggcggcgtt ttctagtctt aatgaccgca cccaggaaag cctcaccagt atccgcatga
    78181 tcaaagcctt tggtctggaa gatcgccagt cggcgttatt tgccgcggat gccgaagata
    78241 ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgacccg accatctata
    78301 tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg tagttggatg gtggtgcagg
    78361 gcagtttaac gctgggccag ctcaccagtt ttatgatgta tttaggtctg atgatttggc
    78421 caatgctggc gctggcatgg atgtttaaca ttgtggaacg tggtagtgct gcatacagcc
    78481 gtattcgcgc gatgctggcg gaagcgccgg tggtgaacga tggtagcgaa cccgtgccgg
    78541 aagggcgtgg cgaactggat gtaaatattc gccagtttac gtatccgcag actgaccatc
    78601 ctgcgctgga aaacgtcaat ttcgctctga aacccggtca gatgctgggt atttgcgggc
    78661 cgactggttc cggcaaaagt accctgttgt cgctcattca acgtcatttc gacgtcagcg
    78721 agggggatat tcgctttcat gatattcctc tgacgaagtt acaactcgat agctggcgta
    78781 gccgcctggc ggtggtcagc cagacgccat tccttttttc tgacaccgtg gcaaataaca
    78841 tcgcgctggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg cggttagcca
    78901 gcgtacatga cgatattttg cgtttaccgc aaggttacga tacagaggtg ggcgagcgcg
    78961 gcgtgatgct ttccggcggg caaaaacagc gtatctctat tgctcgtgcg ttattagtca
    79021 acgcggaaat cctcatcctt gatgatgcgc tttcggcggt ggacggacgc acagagcacc
    79081 agatcctgca taacctgcgt cagtggggac agggaagaac ggtaatcatc agtgcccatc
    79141 gcctttctgc actgacggaa gccagtgaaa ttattgtgat gcagcacgga catatcgccc
    79201 agcgtggcaa tcatgatgtg ctggcgcaac aaagcggctg gtatcgcgat atgtatcgct
    79261 atcaacaact ggaggcggcg ctcgacgacg ctccggaaat tcgcgaggag gccgtcgatg
    79321 cgtagtttta gccaactgtg gccgactctc aagcgcctgt tagcgtacgg ttcaccgtgg
    79381 cgtaaaccgt tggggattgc ggtcctgatg atgtgggttg cggcggcggc agaagtcagt
    79441 gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa cctgccgttg
    79501 aaagtggttg cagggctggc tgcggcgtat gttgggctac aactgtttgc cgccgggcta
    79561 cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca acagttgcgt
    79621 accgacgtga tggatgctgc gttacgccag ccattaagcg agtttgatac ccaacccgtc
    79681 gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgatct ctacgttacc
    79741 gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg cgatgctggt ggcgatgttc
    79801 agcctcgact ggcgaatggc gctggtggcg ataatgattt tcccggtggt gctggtggta
    79861 atggtgatat accagcgtta cagcacgccg attgtccgcc gtgtgcgcgc ctatctggcg
    79921 gatatcaacg acggctttaa cgaaatcatc aatggcatga gcgttatcca gcagttccgt
    79981 cagcaggcgc gatttggcga acgtatgggg gaggccagtc gttcacacta tatggcgagg
    80041 atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct gttttcatcg
    80101 ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ctagcggcac cattgaagtg
    80161 ggcgtgctgt atgcgtttat cagctatctt gggcgactta acgaaccatt aatcgaactg
    80221 accacgcaac aggcgatgct gcaacaggct gttgttgctg gtgagcgcgt gtttgaactg
    80281 atggacggac cgcgccagca atatggcaat gatgatcgcc cgttacagag tggcaccatc
    80341 gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa gaacattaat
    80401 ctctctgtgc cttcgcgtaa ctttgtggca ctggtcgggc ataccggcag tggcaaaagc
    80461 accctcgcca gtttattgat gggctattac ccgctaacgg aaggtgagat tcgccttgat
    80521 ggtcgtccat taagttcgct aagtcacagc gcgctgcgcc agggcgtggc aatggtgcag
    80581 caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg gcgtgatatc
    80641 tccgaagaac gcgtctggca ggcgctggaa accgtgcaac tggcggagct ggcgcgcagc
    80701 atgagtgatg gtatttatac gccgctgggc gagcagggga ataatctctc cgtcgggcaa
    80761 aagcaactgc tggcactggc acgcgtgttg gtggagacgc cgcaaatcct gatccttgat
    80821 gaggcaaccg ccagcattga ctccggtact gaacaggcga ttcaacatgc tctggcggct
    80881 gtgcgtgaac ataccacgct ggtggtgatt gctcaccgct tatcgaccat tgttgatgcc
    80941 gacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca ccagcaactg
    81001 ctggcggccc agggacgcta ctggcagatg tatcaactgc aacttgcggg cgaagagctg
    81061 gcagccagcg tgcgtgaaga agaatcattg agcgcctgaa tagcgcaata tttcatcgtt
    81121 ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt tttcattttc aatcttttta
    81181 acttcctgct ctctttctcg tttttcattt ctggcacacc gtttgcaata ccttcttcgt
    81241 gtagcagaac cattaccgaa ttctgaccgg aggggatcta tgaagctggt gaccgtgata
    81301 atcaaaccat tcaagctgga agacgttcgt gaagcgttat cttccattgg tattcagggc
    81361 ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag ggcatgccga gctgtaccgg
    81421 ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa ttgatgtggc gattgctgat
    81481 gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg cttacaccgg aaaaattggc
    81541 gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc gtattcgtac cggcgaagcc
    81601 gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg aagatagcga
    81661 cgataaaaac tgggcttgct tcactggcga tgctgccggg actggtaatg gctgcacctg
    81721 cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg gtgctgttta
    81781 tgactattcc ggggattgcc ctgttttacg gtgggttgat tcgcggcaaa aacgtgctgt
    81841 cgatgctgac gcaggtgacg gtgacatttg cactggtctg tattctctgg gtggtttacg
    81901 gttactcgct ggcgtttggt gagggcaaca acttcttcgg caacattaac tggttgatgc
    81961 tgaaaaacat cgaactgacg gcggtgatgg gcagcattta tcagtatatc cacgtagcgt
    82021 ttcagggatc gtttgcctgc attaccgtag gcttgatagt tggggcgctg gcggaacgaa
    82081 tccgcttctc agctgtgttg attttcgtgg tggtatggct gacgctctct tacattccga
    82141 ttgcgcatat ggtctggggc ggtggtttgc tggcttctca cggtgcactg gatttcgcgg
    82201 ggggcacagt ggtgcacatt aacgccgcaa tcgccggtct ggtgggcgcg tatctgatag
    82261 gaaaacgcgt gggcttcggt aaagaggcgt ttaaaccgca caacctgccg atggtcttca
    82321 ccgggactgc cattctctat atcggttggt ttggctttaa cgccgggtca gcgggcacgg
    82381 cgaatgaaat cgcggcactg gcttttgtga atactgtggt cgcaacggcg gcggcaattc
    82441 ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttcactgctg ggggcgtgtt
    82501 ctggcgcgat tgccggtctg gtcggcgtga cgccagcctg cggctacatt ggggttggcg
    82561 gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt accatgctca
    82621 aacgcttgct gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc gtttgtggca
    82681 ttgtcggctg tatcatgacc gggatttttg ccgccagctc gctgggcggc gtgggcttcg
    82741 ctgaaggtgt gacgatgggc catcagttgc tggtacagct ggaaagcatt gccattacga
    82801 tcgtctggtc cggtgtggtg gcatttattg gctacaaact ggcggatctg acggttggcc
    82861 tgcgtgtgcc tgaagaacag gagcgagaag ggctggacgt caacagccac ggcgagaatg
    82921 cctataacgc gtaacaagca ctgcaaaaaa cagccggacg gttttcacct ccggctattt
    82981 ttttaattgt gattacgcat taccccttcc tgaacggtcg aggcaaccag cacgccgtcc
    83041 tgggtataaa actcaccgcg cacaaagccg cgtgcgctgg acgccgaggt gctctccacg
    83101 ctatacagca gccattcatt caaattaaac gggcgatgga accacatgga atggtcgatg
    83161 gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt gcggctgtag agctaccggc
    83221 aggaagttaa gatcagaagc gtaaccgaga agatactgat gaacgcgcag atcatccggc
    83281 acgctaccat ttgcgcggat ccacacctga cgatgtggtt ctgcgacgtg acctttcagt
    83341 gggttatgaa actccaccgg acggacttcc agcggacgat cgcagatgaa tttatctttc
    83401 agcactggcg gcagcaggtg cgccagcgat tgggcgattt gcgtttccga aggcaggcca
    83461 tcaggcgctg gcgcggacgg cattgttttt tgatgttcga aacccgcttc tggtgcctgg
    83521 aaagaggccg tcatataaaa aatcggtttg ccgttttgaa tagcagcaac ccggcgggcg
    83581 ctgaagctgt taccgtcacg cagcgtttcg acatcataaa taatcggctt cttactatcg
    83641 ccagggcgaa gaaagtagct gtgaaacgaa tgtaccagcc gctcttcagg gacggtctct
    83701 tttgcagcat acaaggcctg acccacgacc tggccgccaa acacctggcg taaacctaaa
    83761 tcttcactct ggccgcgaaa gagtccttcc tcaatttttt ccagatttaa caatgtcagt
    83821 aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc agcaagccaa
    83881 agtgagttga gtataacgca aatttgctac tggtccgatg ggtgcaatgg tctgaattac
    83941 gggctaatta caggcagaaa tgcgtgatgt gtgccacact tgttgacgtt actattttgt
    84001 taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa ggagaaatga
    84061 atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc ggcttgcgca
    84121 gataaaagcg cggatattca gacgccagcc ccggctgcaa atacgtctat ttcagcaaca
    84181 caacaacctg ctatccagca accgaatgtc tccggtaccg tctggatccg tcagaaagtc
    84241 gcactgccgc ctgatgctgt gctgaccgtg acactttctg acgcgtcgtt agccgatgca
    84301 ccgtcaaaag tgctggcgca gaaagcggtg cgtactgaag gtaaacagtc accattcagc
    84361 tttgttctgc catttaaccc ggcagatgtt cagccgaacg cgcgtattct gttgagtgcg
    84421 gcgattaccg tgaatgacaa actggtattt atcaccgata ccgttcagcc ggtgatcaac
    84481 cagggcggaa ctaaagccga cctgacattg gtgccggtac agcaaaccgc cgtgccggtt
    84541 caggccagcg gtggcgcaac gactaccgta ccttcgactt caccaactca ggtgaatccg
    84601 tcttcggcag ttccagctcc tacgcaatat taagcgacgt taaccctctc cgcccggaga
    84661 gggttagtag ttccagcgat aacgctgcaa gtcgatttgc ccgcttcccg ataccatcac
    84721 accttctgcc agtaatgcct gtcgctgacg ctgtaaatcc ggtccggtta gcgaaattgt
    84781 gccgtggcga ttaaccaccc ggtgccaggg taaggtgctg ccttcaggga gacgctttaa
    84841 cacaccgccc acctggcgcg cggcgcgggg cgatcccgcc agtttcgcca catcaccgta
    84901 agtggtgaca tagccttcgg gaatagcggc gacgatttgc cagacgcgtt ggggaaatga
    84961 atcttctttt tccatctttt cttcctgagg taatttttca gcataatctg gaaaaacgcc
    85021 cgagtgaagt cgcattgcgc aagaaaccag catctggcac gcgatgggtt gcaattagcc
    85081 ggggcagcag tgataatgcg cctgcgcgtt ggttctcaac gctctcaatg ggggctctgt
    85141 tggttctccc gcaacgctac tctgtttacc aggtcaggtc cggaaggaag cagccaaggc
    85201 agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttctg cctcccaccg
    85261 tttcgtcaaa aaatcccaat atggctaaac tttaaccaca actgacgtcg caagaattgt
    85321 ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg ttttgtggtt
    85381 gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc tgcgccattg
    85441 tttaaagagt ttggcgcgct tcgtattgtc gaatgctggg ccagcgatgt accggatggc
    85501 aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt tgtctttagc
    85561 tggattgaat acccttcaaa agaggtccgc gacgctgcta atcaaaagat gatgtcggac
    85621 ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat gatctatggc
    85681 ggattcgagt caatcatcga cgaataggta gcgtgacggg ctgtgcggcg tgccgtcagc
    85741 ccaaaaattc acaaatgttg ctcggcccag cgtaagaaat cttctttcgg taatgccttg
    85801 ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtaaccactc ttcttgttta
    85861 ctggtttcga ttccctccgc taccattttc agtttcagtg tttttgccat ttcgatgata
    85921 tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat cttcagaatg
    85981 tcgacatcca gatcctgtaa ataacttaaa cttgaatacc ccgtaccaaa atcatcaaga
    86041 taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt tttcggatcg
    86101 gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg atagtgattg
    86161 atcatgtcac gcagcaattg cgggattttt tccgaggtga gcacggggga ttcaagattg
    86221 atcgaaatat gctgctgtgg atgctgacgc agccagtcgc ccatatcttc aaagacgctt
    86281 cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag cggaataaaa
    86341 ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc ctcagcaccg
    86401 acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat atcacgattt
    86461 tcgatagcat cctgcagccg atgatgcggt gactgaatac ggcgcaaaat acgcagcaca
    86521 aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa gacttgccga
    86581 tgccagcctt tctgcaacat tttcgttgaa gcccatgtga tgatcgaaat attcatctcc
    86641 ggtaagggca ggatatcgta aatgattcca ttattttcga tatgctcacc gggtgttttt
    86701 tgtagcctgg taataattcc ctgagcaatt tcatcgctgc tggttatgac aacgttatgg
    86761 gcattgccaa taatggcggc atcaatttgc catgagctat agggaatgac atcaataaag
    86821 gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat gtaacggata
    86881 atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga atttttacct
    86941 ggctcgggga aggtatcggg cggactttca tgctccagag acgaacactg gggaacgttg
    87001 ttatcgatat aagccacttc ctgaatatag cgataactgt aagatacccg acgcatttcc
    87061 atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcctg cagcgcatct
    87121 ttcccttgtg tcgccacctt attggctcga atagcgacgc gggaggaata ggtatccagc
    87181 tcttcaataa acgatgtttc tacctgctga tgggccagcc agatgcttaa gccgacaggc
    87241 agcaatactg aaagaatcag tactcccgaa ataaggccga ccagatgtcg tgttctcacc
    87301 agcatatcct tattaacaca gtgtagggaa agtatatctg attgtggaaa aaggctggct
    87361 ggagataagg aggtagagct ggtatttcat gaagtgatgg gtggaaacga tcattgtcat
    87421 ttgaccaggc gcagagcttt acgcctggta tgtaacccta acttaagatg ttgattcggc
    87481 gcgagcgagg gtaaaaatat cgtaaaaaga cagttcacct ttacggacga tcattttttg
    87541 cagttgttcc ttatgaacat ccgcgacacg cggagagttc aagatggctt caaccagcgc
    87601 ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag caaacgcgga aatccagcac
    87661 gtcgatatct tcatagcggt agcgtgggta gacatcgaaa aagaagaaac cattaagcag
    87721 gataaataat acaaccagta gccagacaga gcctgacagc gtttcggact gcaacatcac
    87781 cgccagggtg gcaaaaaaag caacgtacat accgataaac agccccggat gtttacggat
    87841 aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg cgggtattca gagactcaat
    87901 cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc cttcgtggta tctgcaactt
    87961 tccagggaac accctatttt aacgtgggaa tagtagaggg gaagtaacag atctaccaca
    88021 attttgcata acagttgcga aaaaccgatt acaatttttt aattattctg gctggattac
    88081 cgcccacgac aacgttgtcc gggacatctt ttgtgacaac tgcacctgag gctaccacga
    88141 cgttatcacc aatggtcaca ccagggttaa tgaccgcgcg tccgccaatc cagacgttat
    88201 taccgatggt gacgggtttc cccagttcag caccgctatt acgtgctaca gggtcgatgg
    88261 gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat acagttatca ccgatgcgaa
    88321 tagggcagac atcaagcatc acgcaatcga agttggcgaa aaaattatta ccgagaaaaa
    88381 tgttatagcc atagtcacag cgaaacgttg gctcaatata agcctctgtc acctgaccga
    88441 ataaatcagc gagaatttgc tggcgtaatg tgtgctcttc cgccagggaa tgattgtatc
    88501 ggtgaataag ctgacgagcg cgcaggcgat cgcgagataa cgtctcatct gccgagcgat
    88561 acaactcacc agcaatcatc ttttcttttt ctgtgctcat tactgacctc cggttgctga
    88621 aaatgcggca acacagtaag acgaaaagtc agtgaagaga acgttcccat tacatctgtg
    88681 tgataaagat cacatagggt atgtcttcat ggcgaaaaag tataaaattc ttaataaaca
    88741 gccggttata gctccgaaag cgaattattt attagcgaat aaatttccat actgaggaag
    88801 ggatcttgtc gtacagttta ttcatggtca attcggcgag gcggtgatct gcggctgagt
    88861 aaaataccgc cagttcatta tctgataatt cgtatttatt tttctcgata acacgctcca
    88921 gcgtgtcaat tgtctggcaa cgacgtaaac gcattaaata atcggttttc gttaaaggtt
    88981 tttcggacat acttctaccc atggttgtaa taattctaac aagataaact cgcaggattc
    89041 tctttcgtcg cattgacaaa acaacggaat agtcgattac ctgacttccg ccatttctga
    89101 agatcctgca tattaatacc ataactactg aacaacataa aggtgtcatc cagatattcg
    89161 tcgatctgct caatgagctt attgtcttca ttatacttaa ttttgtaatt aagtgcgaag
    89221 gtcgcaatat gctcaatcag ttcattcaac tggaggttga tcgccgaggt tgggtcgtta
    89281 acccagccat gattgctttc ttcaaggttt gcaagacagt catgatacag ggtttcacag
    89341 agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt attcatccat aacgcgtccc
    89401 cttcttagcg gttgaactaa cggacacctt tcgggatgga aaaaacttac tgacctggac
    89461 ttgccctctc gtttgttagc ttaactataa gccactcttt gcaggttttc atcgcattat
    89521 tacgaaaaat tacaattagg aaggaagcgg gacagggctc actttttcat actagcacca
    89581 acccgcagca ggtaaaagca gtttgctcga actgttcata acatttcagg attatcgcca
    89641 gatgaaattg gtgacactaa taccaggatt gctctgaata tgacgtaata accgaggaat
    89701 gaataaagaa ttac
//
LOCUS       DAZFZC010000006        85142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFZC010000006 DAZFZC010000000
VERSION     DAZFZC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.006"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..406)
                     /locus_tag="ACHKXP_000495"
     CDS             complement(<1..406)
                     /locus_tag="ACHKXP_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7645567.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFL"
     gene            complement(477..2591)
                     /gene="fusA"
                     /locus_tag="ACHKXP_000496"
     CDS             complement(477..2591)
                     /gene="fusA"
                     /locus_tag="ACHKXP_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO7645568.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2688..3158)
                     /gene="rpsG"
                     /locus_tag="ACHKXP_000497"
     CDS             complement(2688..3158)
                     /gene="rpsG"
                     /locus_tag="ACHKXP_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO7645569.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3255..3629)
                     /gene="rpsL"
                     /locus_tag="ACHKXP_000498"
     CDS             complement(3255..3629)
                     /gene="rpsL"
                     /locus_tag="ACHKXP_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO7645570.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3755..4042)
                     /gene="tusB"
                     /locus_tag="ACHKXP_000499"
     CDS             complement(3755..4042)
                     /gene="tusB"
                     /locus_tag="ACHKXP_000499"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO7645571.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIILIDYTDFVRLTVKHPSQMAW"
     gene            complement(4050..4409)
                     /gene="tusC"
                     /locus_tag="ACHKXP_000500"
     CDS             complement(4050..4409)
                     /gene="tusC"
                     /locus_tag="ACHKXP_000500"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO7645572.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4409..4795)
                     /gene="tusD"
                     /locus_tag="ACHKXP_000501"
     CDS             complement(4409..4795)
                     /gene="tusD"
                     /locus_tag="ACHKXP_000501"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO7645573.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIADGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRAWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4795..5517)
                     /gene="yheO"
                     /locus_tag="ACHKXP_000502"
     CDS             complement(4795..5517)
                     /gene="yheO"
                     /locus_tag="ACHKXP_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7645574.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5684..6496)
                     /gene="fkpA"
                     /locus_tag="ACHKXP_000503"
     CDS             complement(5684..6496)
                     /gene="fkpA"
                     /locus_tag="ACHKXP_000503"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7645575.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATAADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6717..6935
                     /gene="slyX"
                     /locus_tag="ACHKXP_000504"
     CDS             6717..6935
                     /gene="slyX"
                     /locus_tag="ACHKXP_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO7645576.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6984..7574)
                     /gene="slyD"
                     /locus_tag="ACHKXP_000505"
     CDS             complement(6984..7574)
                     /gene="slyD"
                     /locus_tag="ACHKXP_000505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7645577.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7669..7869)
                     /gene="yheV"
                     /locus_tag="ACHKXP_000506"
     CDS             complement(7669..7869)
                     /gene="yheV"
                     /locus_tag="ACHKXP_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO7645578.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7879..9684)
                     /gene="kefB"
                     /locus_tag="ACHKXP_000507"
     CDS             complement(7879..9684)
                     /gene="kefB"
                     /locus_tag="ACHKXP_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO7645579.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKVGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     TTQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9684..10235)
                     /gene="kefG"
                     /locus_tag="ACHKXP_000508"
     CDS             complement(9684..10235)
                     /gene="kefG"
                     /locus_tag="ACHKXP_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO7645580.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10366..12279
                     /gene="yheS"
                     /locus_tag="ACHKXP_000509"
     CDS             10366..12279
                     /gene="yheS"
                     /locus_tag="ACHKXP_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7645581.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSHIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12279..13301
                     /gene="yheT"
                     /locus_tag="ACHKXP_000510"
     CDS             12279..13301
                     /gene="yheT"
                     /locus_tag="ACHKXP_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7645582.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13295..13513
                     /gene="yheU"
                     /locus_tag="ACHKXP_000511"
     CDS             13295..13513
                     /gene="yheU"
                     /locus_tag="ACHKXP_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO7645583.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13567..14436
                     /gene="prkB"
                     /locus_tag="ACHKXP_000512"
     CDS             13567..14436
                     /gene="prkB"
                     /locus_tag="ACHKXP_000512"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO7645584.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14491..14895)
                     /gene="yhfA"
                     /locus_tag="ACHKXP_000513"
     CDS             complement(14491..14895)
                     /gene="yhfA"
                     /locus_tag="ACHKXP_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO7645585.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15197..15829
                     /gene="crp"
                     /locus_tag="ACHKXP_000514"
     CDS             15197..15829
                     /gene="crp"
                     /locus_tag="ACHKXP_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO7645586.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15880..17970
                     /gene="yhfK"
                     /locus_tag="ACHKXP_000515"
     CDS             15880..17970
                     /gene="yhfK"
                     /locus_tag="ACHKXP_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO7645587.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18037..19257)
                     /gene="argD"
                     /locus_tag="ACHKXP_000516"
                     /gene_synonym="dapC"
     CDS             complement(18037..19257)
                     /gene="argD"
                     /locus_tag="ACHKXP_000516"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO7645588.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19343..19906)
                     /gene="pabA"
                     /locus_tag="ACHKXP_000517"
     CDS             complement(19343..19906)
                     /gene="pabA"
                     /locus_tag="ACHKXP_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO7645589.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19938..20540)
                     /gene="fic"
                     /locus_tag="ACHKXP_000518"
     CDS             complement(19938..20540)
                     /gene="fic"
                     /locus_tag="ACHKXP_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO7645590.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20530..20697)
                     /gene="yhfG"
                     /locus_tag="ACHKXP_000519"
     CDS             complement(20530..20697)
                     /gene="yhfG"
                     /locus_tag="ACHKXP_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO7645591.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20802..21374)
                     /gene="ppiA"
                     /locus_tag="ACHKXP_000520"
                     /gene_synonym="rotA"
     CDS             complement(20802..21374)
                     /gene="ppiA"
                     /locus_tag="ACHKXP_000520"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO7645592.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21645..22826
                     /gene="tsgA"
                     /locus_tag="ACHKXP_000521"
     CDS             21645..22826
                     /gene="tsgA"
                     /locus_tag="ACHKXP_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO7645593.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23088..25631
                     /gene="nirB"
                     /locus_tag="ACHKXP_000522"
     CDS             23088..25631
                     /gene="nirB"
                     /locus_tag="ACHKXP_000522"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO7645594.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVLCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25628..25954
                     /gene="nirD"
                     /locus_tag="ACHKXP_000523"
     CDS             25628..25954
                     /gene="nirD"
                     /locus_tag="ACHKXP_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO7645595.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26081..26887
                     /gene="nirC"
                     /locus_tag="ACHKXP_000524"
     CDS             26081..26887
                     /gene="nirC"
                     /locus_tag="ACHKXP_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO7645596.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26906..28279
                     /gene="cysG"
                     /locus_tag="ACHKXP_000525"
     CDS             26906..28279
                     /gene="cysG"
                     /locus_tag="ACHKXP_000525"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO7645597.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28526..28693
                     /gene="yhfL"
                     /locus_tag="ACHKXP_000526"
     CDS             28526..28693
                     /gene="yhfL"
                     /locus_tag="ACHKXP_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO7645598.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28988..30325
                     /gene="frlA"
                     /locus_tag="ACHKXP_000527"
     CDS             28988..30325
                     /gene="frlA"
                     /locus_tag="ACHKXP_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO7645599.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30346..31368
                     /gene="frlB"
                     /locus_tag="ACHKXP_000528"
     CDS             30346..31368
                     /gene="frlB"
                     /locus_tag="ACHKXP_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO7645600.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31418..32248
                     /gene="frlC"
                     /locus_tag="ACHKXP_000529"
     CDS             31418..32248
                     /gene="frlC"
                     /locus_tag="ACHKXP_000529"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO7645601.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32245..33030
                     /gene="frlD"
                     /locus_tag="ACHKXP_000530"
     CDS             32245..33030
                     /gene="frlD"
                     /locus_tag="ACHKXP_000530"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO7645602.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33130..33861
                     /gene="frlR"
                     /locus_tag="ACHKXP_000531"
                     /gene_synonym="yhfR"
     CDS             33130..33861
                     /gene="frlR"
                     /locus_tag="ACHKXP_000531"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO7645603.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34013..35098)
                     /gene="yhfS"
                     /locus_tag="ACHKXP_000532"
     CDS             complement(34013..35098)
                     /gene="yhfS"
                     /locus_tag="ACHKXP_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7645604.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35110..36414)
                     /gene="yhfT"
                     /locus_tag="ACHKXP_000533"
     CDS             complement(35110..36414)
                     /gene="yhfT"
                     /locus_tag="ACHKXP_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO7645605.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36426..36779)
                     /gene="yhfU"
                     /locus_tag="ACHKXP_000534"
     CDS             complement(36426..36779)
                     /gene="yhfU"
                     /locus_tag="ACHKXP_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO7645606.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36790..37668)
                     /gene="php"
                     /locus_tag="ACHKXP_000535"
     CDS             complement(36790..37668)
                     /gene="php"
                     /locus_tag="ACHKXP_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO7645607.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37665..38891)
                     /gene="yhfW"
                     /locus_tag="ACHKXP_000536"
     CDS             complement(37665..38891)
                     /gene="yhfW"
                     /locus_tag="ACHKXP_000536"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7645608.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38891..40054)
                     /gene="yhfX"
                     /locus_tag="ACHKXP_000537"
     CDS             complement(38891..40054)
                     /gene="yhfX"
                     /locus_tag="ACHKXP_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO7645609.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(40138..40500)
                     /gene="yhfY"
                     /locus_tag="ACHKXP_000538"
     CDS             complement(40138..40500)
                     /gene="yhfY"
                     /locus_tag="ACHKXP_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO7645610.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40517..41422)
                     /gene="yhfZ"
                     /locus_tag="ACHKXP_000539"
     CDS             complement(40517..41422)
                     /gene="yhfZ"
                     /locus_tag="ACHKXP_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO7645611.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELANECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41712..42716)
                     /gene="trpS"
                     /locus_tag="ACHKXP_000540"
     CDS             complement(41712..42716)
                     /gene="trpS"
                     /locus_tag="ACHKXP_000540"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO7645612.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42709..43467)
                     /gene="gph"
                     /locus_tag="ACHKXP_000541"
     CDS             complement(42709..43467)
                     /gene="gph"
                     /locus_tag="ACHKXP_000541"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO7645613.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43460..44137)
                     /gene="rpe"
                     /locus_tag="ACHKXP_000542"
     CDS             complement(43460..44137)
                     /gene="rpe"
                     /locus_tag="ACHKXP_000542"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO7645614.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44155..44991)
                     /gene="dam"
                     /locus_tag="ACHKXP_000543"
     CDS             complement(44155..44991)
                     /gene="dam"
                     /locus_tag="ACHKXP_000543"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7645615.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45098..46384)
                     /gene="damX"
                     /locus_tag="ACHKXP_000544"
     CDS             complement(45098..46384)
                     /gene="damX"
                     /locus_tag="ACHKXP_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO7645616.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKSQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGAKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46476..47564)
                     /gene="aroB"
                     /locus_tag="ACHKXP_000545"
     CDS             complement(46476..47564)
                     /gene="aroB"
                     /locus_tag="ACHKXP_000545"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO7645617.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47621..48142)
                     /gene="aroK"
                     /locus_tag="ACHKXP_000546"
     CDS             complement(47621..48142)
                     /gene="aroK"
                     /locus_tag="ACHKXP_000546"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO7645618.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48543..49781)
                     /gene="hofQ"
                     /locus_tag="ACHKXP_000547"
     CDS             complement(48543..49781)
                     /gene="hofQ"
                     /locus_tag="ACHKXP_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO7645619.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSNE"
     gene            complement(49693..50097)
                     /gene="hofP"
                     /locus_tag="ACHKXP_000548"
     CDS             complement(49693..50097)
                     /gene="hofP"
                     /locus_tag="ACHKXP_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO7645620.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50087..50527)
                     /gene="hofO"
                     /locus_tag="ACHKXP_000549"
                     /pseudo
     CDS             complement(50087..50527)
                     /gene="hofO"
                     /locus_tag="ACHKXP_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
     gene            complement(50511..51050)
                     /gene="hofN"
                     /locus_tag="ACHKXP_000550"
     CDS             complement(50511..51050)
                     /gene="hofN"
                     /locus_tag="ACHKXP_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO7645621.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(51050..51829)
                     /gene="hofM"
                     /locus_tag="ACHKXP_000551"
     CDS             complement(51050..51829)
                     /gene="hofM"
                     /locus_tag="ACHKXP_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO7645622.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51949..54501
                     /gene="mrcA"
                     /locus_tag="ACHKXP_000552"
     CDS             51949..54501
                     /gene="mrcA"
                     /locus_tag="ACHKXP_000552"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO7645623.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54667..55227)
                     /gene="nudE"
                     /locus_tag="ACHKXP_000553"
     CDS             complement(54667..55227)
                     /gene="nudE"
                     /locus_tag="ACHKXP_000553"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO7645624.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55547..57682
                     /gene="igaA"
                     /locus_tag="ACHKXP_000554"
     CDS             55547..57682
                     /gene="igaA"
                     /locus_tag="ACHKXP_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO7645625.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     SYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57747..58415
                     /gene="yrfG"
                     /locus_tag="ACHKXP_000555"
     CDS             57747..58415
                     /gene="yrfG"
                     /locus_tag="ACHKXP_000555"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO7645626.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58426..58827
                     /gene="hslR"
                     /locus_tag="ACHKXP_000556"
     CDS             58426..58827
                     /gene="hslR"
                     /locus_tag="ACHKXP_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO7645627.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58852..59730
                     /gene="hslO"
                     /locus_tag="ACHKXP_000557"
     CDS             58852..59730
                     /gene="hslO"
                     /locus_tag="ACHKXP_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO7645628.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59793..61517)
                     /gene="yhgE"
                     /locus_tag="ACHKXP_000558"
     CDS             complement(59793..61517)
                     /gene="yhgE"
                     /locus_tag="ACHKXP_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO7645629.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLSGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQPVSEKV
                     LADNVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLNVNLNE"
     gene            61896..63518
                     /gene="pckA"
                     /locus_tag="ACHKXP_000559"
     CDS             61896..63518
                     /gene="pckA"
                     /locus_tag="ACHKXP_000559"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO7645630.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63594..64946)
                     /gene="envZ"
                     /locus_tag="ACHKXP_000560"
     CDS             complement(63594..64946)
                     /gene="envZ"
                     /locus_tag="ACHKXP_000560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO7645631.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64943..65662)
                     /gene="ompR"
                     /locus_tag="ACHKXP_000561"
     CDS             complement(64943..65662)
                     /gene="ompR"
                     /locus_tag="ACHKXP_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO7645632.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65890..66366
                     /gene="greB"
                     /locus_tag="ACHKXP_000562"
     CDS             65890..66366
                     /gene="greB"
                     /locus_tag="ACHKXP_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO7645633.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66463..68784
                     /gene="yhgF"
                     /locus_tag="ACHKXP_000563"
     CDS             66463..68784
                     /gene="yhgF"
                     /locus_tag="ACHKXP_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO7645634.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69222..69449
                     /gene="feoA"
                     /locus_tag="ACHKXP_000564"
     CDS             69222..69449
                     /gene="feoA"
                     /locus_tag="ACHKXP_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO7645635.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69466..71787
                     /gene="feoB"
                     /locus_tag="ACHKXP_000565"
     CDS             69466..71787
                     /gene="feoB"
                     /locus_tag="ACHKXP_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO7645636.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71787..72023
                     /gene="feoC"
                     /locus_tag="ACHKXP_000566"
     CDS             71787..72023
                     /gene="feoC"
                     /locus_tag="ACHKXP_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO7645637.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72226..73104
                     /gene="rpnA"
                     /locus_tag="ACHKXP_000567"
     CDS             72226..73104
                     /gene="rpnA"
                     /locus_tag="ACHKXP_000567"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO7645638.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73133..73903)
                     /gene="bioH"
                     /locus_tag="ACHKXP_000568"
     CDS             complement(73133..73903)
                     /gene="bioH"
                     /locus_tag="ACHKXP_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO7645639.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73941..74624
                     /gene="gntX"
                     /locus_tag="ACHKXP_000569"
     CDS             73941..74624
                     /gene="gntX"
                     /locus_tag="ACHKXP_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO7645640.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74683..75258
                     /gene="nfuA"
                     /locus_tag="ACHKXP_000570"
     CDS             74683..75258
                     /gene="nfuA"
                     /locus_tag="ACHKXP_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO7645641.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75618..76934
                     /gene="gntT"
                     /locus_tag="ACHKXP_000571"
     CDS             75618..76934
                     /gene="gntT"
                     /locus_tag="ACHKXP_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7645642.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77045..79129)
                     /gene="malQ"
                     /locus_tag="ACHKXP_000572"
     CDS             complement(77045..79129)
                     /gene="malQ"
                     /locus_tag="ACHKXP_000572"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO7645643.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79139..81532)
                     /gene="malP"
                     /locus_tag="ACHKXP_000573"
     CDS             complement(79139..81532)
                     /gene="malP"
                     /locus_tag="ACHKXP_000573"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO7645644.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82144..84849
                     /gene="malT"
                     /locus_tag="ACHKXP_000574"
     CDS             82144..84849
                     /gene="malT"
                     /locus_tag="ACHKXP_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO7645645.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREDTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQDAVQHAQQLLKMMGYGV"
     gene            complement(84892..>85142)
                     /locus_tag="ACHKXP_000575"
     CDS             complement(84892..>85142)
                     /locus_tag="ACHKXP_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO7645646.1"
                     /translation="VSAEVVAAQLVKEVKRYLASTAAVGEYLADQLVLPMALAGAGEF
                     TVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTRVSIE"
BASE COUNT        20320 a        23188 c        21585 g        20049 t
ORIGIN      
        1 tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg tgctcacgag
       61 tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc atctgagcag
      121 caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg tgtgcgtagt
      181 gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca cgagcttttt
      241 cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag gttttagcca
      301 gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg ccgatagtac
      361 caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct atattcctta
      421 ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc ttaggcttat
      481 ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc atacttcagg
      541 aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt tgcgtatccg
      601 aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc agattcctga
      661 cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt gttctcttcc
      721 ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc tttcttaaag
      781 ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga gtcaacgtca
      841 tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc tgccagcgga
      901 cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc gccagggatt
      961 acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc cggctccagc
     1021 gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg tttcgcgtgt
     1081 ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac ctgcggttta
     1141 cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat gtcgaggtgc
     1201 agttcgccca tacccgcgat gatggtctgg ttagattctt cgtcagtcca tacacggaaa
     1261 gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg gtcagctttg
     1321 gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg ttccagaatg
     1381 atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag accgatagca
     1441 gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc gtgcatctga
     1501 acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt atcaccagag
     1561 ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg gtcggtagcg
     1621 attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgttc agccggagtg
     1681 tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga tggcaggtaa
     1741 tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga accacaggtt
     1801 accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat ttctgcttca
     1861 gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc agctgcggat
     1921 tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg gatatcttcg
     1981 tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat tttcaccagg
     2041 tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg aaccgggttc
     2101 gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc catgcggtcc
     2161 attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg ccatacggtt
     2221 tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc atcgagaaca
     2281 cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc gatgatgttg
     2341 atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt cgcagcggaa
     2401 gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc agcgccgtca
     2461 tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc ggtagtagtg
     2521 gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc gatgggtgtt
     2581 gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta gcccaaagcg
     2641 ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta ccaacggtag
     2701 tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt cttaactgca
     2761 gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc catggattta
     2821 tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt acgacgaacc
     2881 ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga cttaacttct
     2941 acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga tttaccagag
     3001 cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga ttttttacca
     3061 tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt cggatccggc
     3121 agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg ttgttaattc
     3181 aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt ttggccttac
     3241 ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg agcctgctta
     3301 cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg aacacccggg
     3361 aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag gttgtgacct
     3421 tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg gcatacttta
     3481 cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca tacgccacgt
     3541 ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg agcacgtggt
     3601 ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc ttttgcttcg
     3661 taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag ggcgatgccg
     3721 aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg ctggggtgct
     3781 taaccgtaag tctgacgaaa tcagtatagt caatgagaat gatgtcgttc gaaatttgac
     3841 cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg ggggcattac
     3901 gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct tgcaataata
     3961 gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc agccagggtg
     4021 agcgatgtaa tgtgtgcagc ataaacgcct caaaacctca aaataacatc gtagttggcg
     4081 agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa gggtgtctgc
     4141 ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc aatgtcgtac
     4201 agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc gggcttttgt
     4261 cctggcagca gctgaaaaac gccatcagct ataaagaaga cagccagatc gtcagttaat
     4321 gcggaagttg ccagtaaagc atctaaacct tcccggcctg cggctgtacc atgaggtgca
     4381 gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca caggtcagcg
     4441 aggcttccgc cagcgcccca agtccgctta aggtaaatcc ctgctgaagg tttgacgaag
     4501 ccagccccag tcttccggcc tccgtttcat caacaacgcc acggcgtaat gctgccgcta
     4561 cgcagatatt cagcgccaca ccatgttgcg cattcagttg ttgccaggcc cgtacgaggt
     4621 caaattcgtc acttgccgga gaggtcaatt ggttagcgtt atagacccct tcccgataga
     4681 aaaagacgct gcttaactca tggccatctg ctatcagcgc ctgcgcaaac tgaaaagcac
     4741 tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa cgcattactt
     4801 atcttgcccc tggaaatcac cgctcttgaa ctggcggatg tagagataga cagtgtgttt
     4861 ggagatgttc aggcggtcag caacctggtt gatcgcatct ttaatatcga agatcccttt
     4921 ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat tgcgatcggc
     4981 attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa cagaagaggc
     5041 aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaggtgc tcataatctg
     5101 cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac gctgttcgcg
     5161 gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc gagtaaagta
     5221 gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta gcgccaggtc
     5281 agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc gaatggctga
     5341 acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa taagcatcgc
     5401 taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct ggtcgaaagg
     5461 acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc tggacatgaa
     5521 aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc attaatgatg
     5581 cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat atccggcctg
     5641 taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt ttagcagaat
     5701 ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt ttcacatcca
     5761 gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca cccgctttgc
     5821 cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg atgttcttca
     5881 gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt tcaccacggg
     5941 tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc actacaacag
     6001 tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa accagaccag
     6061 ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct ttaccttttg
     6121 cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc acgcgagctt
     6181 cgaatgcttg tagagtctgt tcgatctctt ggtcggagag tttgctctta tcagcaaatg
     6241 catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt ttttcttgtt
     6301 cttttagaga gttttccatg taacgaccca gcgaggcacc cagtgcataa gctgatttct
     6361 gatcgtcatt tttgaacgct gctttgctgt cagcagctgt agcaggtttt gcagcttcag
     6421 cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc agcgttactt
     6481 taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg gttaactatt
     6541 atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg ccctattatc
     6601 atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat gagtgctgtc
     6661 tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg tgaatcatgc
     6721 aggatttatc attggaagca cgcctggcag agctggagag ccgactggct tttcaggaaa
     6781 tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg gcgaaactgc
     6841 gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg aacatcgcgt
     6901 cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg gggattcccc
     6961 gcttttttgt cactttttcg gtattagtgg caaccgcaac cgccgttgcc tttaccgcca
     7021 cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc gcagcaaccg
     7081 tcgtggtcgt gatcgtggtg gtgatcgtgc gcgccgtgaa cgtgaccatg agccagttct
     7141 tcttcagtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt ctgaccggcc
     7201 agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat ttcaaccggt
     7261 accggaccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc atcaacgccc
     7321 ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc gtaagcgtcg
     7381 ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag cgccgtttcc
     7441 aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact caccggagac
     7501 tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac caccaggtct
     7561 tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga ttgtagcgga
     7621 attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct agtccggatg
     7681 aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt tgtctgcttc
     7741 tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt cgcgccacat
     7801 cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcaataa aacgttttcg
     7861 gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc gtcgttcttg
     7921 ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg cgcgagaaat
     7981 ttgtacggta tcggcatgca ttgggatgag ctctcgcagc atccgcatat ccaggcggcg
     8041 aaaatgcagt tgcgctcgct gtgcctgatg cggatgcatg ccaagcgtga ccagcgtctt
     8101 gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca cccctgcctg
     8161 taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca aatgcggaaa
     8221 gtgctgttgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac aggtaatgac
     8281 gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg tggtgtcgcc
     8341 gtaataaact ttgtagccgt atttacgcat caggttaacg gcgctgatat cccgctccag
     8401 cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac caaaacgccc
     8461 gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt cgtcttcttc
     8521 ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca gcaacggcgt
     8581 ggtcatcatg gaaagcgtca ccgtcaccag caacaacgcc atctggtcgc cctggaataa
     8641 gcgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccaccct gactcaacac
     8701 gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca gcagatacag
     8761 cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca acagatgggt
     8821 ataaagcacc ccgaggttga gtgacatgcc gacagagata aagaacaaac cgagcagcaa
     8881 gcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt ccgccagcag
     8941 cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca taaacaatgc
     9001 ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc cagaagctgc
     9061 aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc cgacaaacgc
     9121 cagcaccttc atgccgacct tcatccagtc gaaatgttcg tctgccgacc ccgccaacaa
     9181 cggcactaac gccagtgctg ggattactgc cagatcctga aacagcagaa ccgaaaaccc
     9241 gagctggccg gattcgctgc gattcatccc tttctcacgc atcaattgca acgccattgc
     9301 agttgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg cgaaatccgt
     9361 cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg cgcctacgcc
     9421 aaaaatcgaa cgccgcagtt gccaaagttt ggagggattc aactcaaggc cgatgataaa
     9481 catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt cgctaataaa
     9541 ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca cagcgccaat
     9601 acccagccgc gatgccagcg gcaccgcagc caccgccgcg aagagaaaca gcactcctgc
     9661 gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt tgccagccag
     9721 tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct cgcccagtaa
     9781 ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc aaaggggcgc
     9841 agcacatcgc tcatcgggta gcgattcagc gcgtcataac ggtaagcact ttccggctcg
     9901 ccggtggtaa tcacgctacg ccagtacttt cccgccagtt ggtttcctcc cggcccgctg
     9961 gcaaaaccac gacttaatac ccggtccagc cactctttca gtagcgccgg gcagctatag
    10021 gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc ctgctcacgg
    10081 gggatatcaa taaaaaaatc gggatagtgc gcgtaaaggt cgtgcacggt aacattgctg
    10141 agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga ttccggatgg
    10201 gcatacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt atggtttttg
    10261 tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc catacattac
    10321 attatcataa tgataagtta acatagtctg aacatacggc accttatgat tgttttctcc
    10381 tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc caccatcaac
    10441 cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac cctgctggca
    10501 ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg aagctggcaa
    10561 ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga atatgtcatt
    10621 gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa cgaacgtaac
    10681 gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc atggagtatt
    10741 cgctcccgtg ctgccagcct gctgcacggc ctcggtttca gcaatgaaca actggagcgc
    10801 ccggtaagtg atttttccgg gggctggcgt atgcgtctta accttgccca ggcgctgatt
    10861 tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct cgatgccgtt
    10921 atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat ctctcacgac
    10981 cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca aagcatgttc
    11041 gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct ggcgcagcaa
    11101 caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta tatcgaccgt
    11161 ttccgtgcca aagccaccaa agcgaagcag gcccagagcc acattaagat gctcgagcgt
    11221 atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt ccgcgcgccg
    11281 gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta tggcgatcgc
    11341 attattctcg actcgattaa actgaacctg gtgcccggct cgcgtattgg tctgttaggc
    11401 cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact tgcgccagtc
    11461 agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca gcatcaactt
    11521 gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc gccgcaggag
    11581 ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga taaagtaacc
    11641 gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc attaattgtc
    11701 tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga cctcgacatg
    11761 cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt cgtttcgcac
    11821 gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga tcgtaaagtc
    11881 gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt acaaaagcag
    11941 gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc acgtaaagat
    12001 cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa agagattgcc
    12061 cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga agagaaactc
    12121 ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg cctgcaacag
    12181 caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga agcccaggag
    12241 cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata acgacgaccg
    12301 atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt agcaattgtc
    12361 atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc ccgtactggc
    12421 agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa aaccctgcac
    12481 aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt ctcaacagcc
    12541 cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc gtggtgatgc
    12601 attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat tcgggcgaaa
    12661 ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat gcgccaacgg
    12721 ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca aaggaaggca
    12781 atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg gaagcctgta
    12841 gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg aacctgttaa
    12901 aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt aatctcgcgc
    12961 agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc agaattcacg
    13021 gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg ctgaaccgga
    13081 tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat catcaggtga
    13141 tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa catggcggtc
    13201 atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag tcacgcattc
    13261 ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc aagacctctc
    13321 ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca ccgattatgg
    13381 tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc tacagtgcgg
    13441 agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc cgcgcagtca
    13501 gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct aagtcatgga
    13561 gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg cgcggggacc
    13621 accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca tgcagctgag
    13681 gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc gatccgcaaa
    13741 gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga cttcggcctg
    13801 ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg caaatatctg
    13861 catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac cccctggcaa
    13921 cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg cgtagtcacg
    13981 ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc tatcgttaac
    14041 cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc acgagaagca
    14101 gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac accgcagttt
    14161 tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa cccgttcgcg
    14221 gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg taatctggaa
    14281 gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc ccacatcaat
    14341 acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat gctgccgctg
    14401 gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc cggataaaat
    14461 actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat acgagtgagt
    14521 aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg cagagagatc
    14581 aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa aatgcagatt
    14641 aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt ttacttcaca
    14701 atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg cactgcaacc
    14761 acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg agttgccgtc
    14821 cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt cgacccactt
    14881 cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga gtacgcgtac
    14941 taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca ccaggagaca
    15001 caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga tgtactgcat
    15061 gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc caggtagcgg
    15121 gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga aagcttataa
    15181 cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc tcgaatggtt
    15241 cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc accagggtga
    15301 aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga tcaaagacga
    15361 agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg gcgaactggg
    15421 cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg cctgtgaagt
    15481 ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg acattctgat
    15541 gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag tgggcaacct
    15601 ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg caaaacaacc
    15661 agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg aaattggtca
    15721 gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg aagatcagaa
    15781 cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat cccgtcggag
    15841 tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag actgatttat
    15901 caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc cgtggccgtt
    15961 gggttaatgc ttggcgaatt acgattcggt ctgctcttct ccctcgttcc tgcctgttgc
    16021 aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat cattggtgcg
    16081 tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga tgttcccctg
    16141 ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagct ggggccattg
    16201 cacgcaaaat tgcttcctgc atcgctgctc gccgccattt ttaccctcag tttggcggga
    16261 tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg gtacggattg
    16321 tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc actaagtctg
    16381 ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac ccagcacacc
    16441 gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt cgatctaatt
    16501 acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga ctacaagcgg
    16561 atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt cagtttgcat
    16621 cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt tatccgctgg
    16681 aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct ttaccatcgc
    16741 ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat cgcccgccag
    16801 catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat cgcccgcgtg
    16861 ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg ccgataaaca gcggcgaatg
    16921 ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct acgcaatgcc
    16981 ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct gcatctgccg
    17041 aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta tggcgcaacc
    17101 cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat tgcgggcgtg
    17161 gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat taccaccctc
    17221 gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt tactattacc
    17281 gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct tccgcgtctt
    17341 atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct gtggccgcag
    17401 tggcagagcg ggttattgcg taaaaacgcc catgatgctt tagaagccta tcaggaagcg
    17461 attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca gcgaatgcgg
    17521 gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca ggaaccggcg
    17581 tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca gtttattgtt
    17641 gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc acctgaactg
    17701 gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca gcgactggag
    17761 tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga gatgcagccg
    17821 cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg tcatctgaac
    17881 accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg gatttggctg
    17941 agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat aaggcattca
    18001 cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca ccttcgccac
    18061 cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca ccagcgacgg
    18121 tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct ctgcgcccgc
    18181 atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc caatcaacag
    18241 ccccataccg cgaatatcgc taaatacatc gtactgctga tcgatcttct gcagatggtc
    18301 aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat tgatgatatc
    18361 aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg aaccgtgaga
    18421 accaggatga aacgcagaag caatttccgc cgtggtcagc atggcgctaa tcgggaagcc
    18481 gccgcctaac gctttcgcag aggtcagaat atccggcgta acgccgtagt gcatgtaagc
    18541 aaacaaatcg ccggtccgcc ccatcccgca ctgcacttca tcaaacacca ataatgcctg
    18601 atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg ccgtcacacc
    18661 gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca tcaccgcttt
    18721 cactgcatgg agatcgttaa agggaacgtg gatgatgtct gccggtttcg gcccaaagcc
    18781 gtcggaatat tttggctgcc cacccaccga aacggtaaac agcgagcgac catgaaaagc
    18841 gttatggaag gcaataattt tggttttgaa cgggctatga cgcacacagg cgtaatggcg
    18901 tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca ccacgcgttc
    18961 ggcaaacgtt gcctcaatca gtttacgccc aagacgcagc gccggttcat tggtgaaaac
    19021 gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca gcgcaggatg
    19081 gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact ccttgccttg
    19141 ctgatcccag attcggctgc cctgaccttt taccggaata aactctgccg gagcataaat
    19201 cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa ttgccatctc
    19261 atgatcaccc tgttacgcat aaacaaatgt gaaattataa ccacaaaata tgcataaaaa
    19321 atcactaaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt tgatgtcctt
    19381 gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac tggcgatggc
    19441 gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcac gctggtaatg
    19501 agtcaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt gccagccccc
    19561 gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg acctttgcgg
    19621 cgcgcacaac tttaccgcca aatgcctgcg ccattgcctg atgaccgagg cagacgccaa
    19681 gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg gcttcatctg
    19741 gcgtacaggg gccaggtgag atgacaattt tttgtggttt aagggcgtcg atatccgcca
    19801 gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa aagtactggt
    19861 agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg gctccggtac
    19921 aaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt gctaaatatc
    19981 atctgcagtg cggtgagatc ccccattgcc ccactctgat ttgcctgatt ccaggcctct
    20041 ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg ctcgaagaag
    20101 atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat ttcacaatag
    20161 taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata accttcttcc
    20221 tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg gcagaacggc
    20281 gtatcaccct gataaatatc aacttcacgc agttgccctg cccagtcgaa aatatcctga
    20341 tacagctggc gatggatagt tcgcaaatgc ggtaaaccgc gcaccagcgg accaagctca
    20401 atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg ctgctgctgg
    20461 cggatgttca gccggttacg catgatatca aggcctggat aaagatacgg atcgcgccct
    20521 tcgccgaatt tatcgctcat agtgactcct cagctcttca aggcgcgcta aagcctctgc
    20581 ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg cctgaaaatt
    20641 acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt tcttcacttg
    20701 atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg gttacgccgg
    20761 gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc attacggcag gactttagcg
    20821 gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc atgagtcggc
    20881 acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa taccgcgtaa
    20941 ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat aaagaactgg
    21001 ctggtggcgc tgtctttgtc agcggtacgt gccatcgcga tggtgccacg cgtgttgcgc
    21061 aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg catctgctcg
    21121 gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt agtgttgtta
    21181 taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc tttttgttta
    21241 tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg gtcccctttc
    21301 gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat cgccgccagg
    21361 gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa gtgaagagat
    21421 tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat ctgaaatata
    21481 tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa tgcaatttag
    21541 caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc gcccgctcgc
    21601 ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact aacagcaatc
    21661 gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg ttggttattg
    21721 tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt tccagtatga
    21781 gtaatacctt caccttcctc aacgccggca ttttaatctc tatcttcctc aacgcctggc
    21841 tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg atggtgctgg
    21901 cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg atgttcattc
    21961 tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca caaatgtatg
    22021 aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt atggctggga
    22081 tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg tactgggttt
    22141 atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc tgtgagttcc
    22201 cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa aagtggggga
    22261 tcggcgtact gtttctctct gttgcggcgt tgtgctacat cctcggtcag ttaggtttta
    22321 tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac gcgggcacgc
    22381 tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc tttattcttc
    22441 gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg attctgatgt
    22501 acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct ctgggcttct
    22561 tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc aaagtaccgt
    22621 cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg ttgacctttg
    22681 tggttaccgg cccgatcgtt gaacatagcg gtccgcaggc ggcactgctt acagcaaacg
    22741 gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc cgtcaccgtc
    22801 agcataacac cctgacctct cattaattgc tcatgccgga cggcactatc gtcgtccggc
    22861 cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgctca ttttgtctat
    22921 tttttgcaca aacatgaaat atcagacaat tccgtgactt aagaaaattt atacaaatca
    22981 gcaatatacc cattaaggag tatataaagg tgaatttgat ttacatcaat aagcggggtt
    23041 gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg agcaaagtca
    23101 gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat cttcttgata
    23161 aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc atcgcttatg
    23221 accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg tcgctggtgc
    23281 gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc gctatcacca
    23341 tcaaccgtca ggagaaggtg attcactcca gcgccggacg taccgttttt tatgacaagc
    23401 tgatcatggc aaccggttcc tacccgtgga tcccgccaat caaaggttct gatactcagg
    23461 actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc gcccgtcgca
    23521 gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgca ggcgcgctga
    23581 aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg gcagaacagc
    23641 ttgatcagat gggcggcgag cagctgcgtc gcaaaatcga aagtatgggc gtgcgcgttc
    23701 acaccagcaa aaacaccctt gagattgtgc aggaaggtgt tgaagcgcgt aaaaccatgc
    23761 gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc ggtatccgtc
    23821 cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg ggtattgtca
    23881 ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa tgcgcaagct
    23941 ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag gtcgccgttg
    24001 accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc aagctgaaac
    24061 tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct ggcgcacgta
    24121 gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc agcgaagaca
    24181 acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggt aacctgctgc
    24241 aactggtgct gaacgctatc gaactgccgg aaaacccgga ttccctgatc ctgccagcac
    24301 actcgggtag cggcaagccg tctatcggtg ttgataaact gccggacagc gcgcaaatct
    24361 gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa ggctgccaca
    24421 cagttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc tgtatcccgc
    24481 tggtcactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt aacaacaacc
    24541 tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc gttgaaggca
    24601 ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt gaagtttgta
    24661 aaccaaccgt cggttcgctg ctggcctcct gctggaacga atacattctg aagccggaac
    24721 atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa gacggcacct
    24781 actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg atggcggtag
    24841 gtcgtatcgc gcgtgaattt aatctctaca ccaagatcac tggctcccag cgtctggcga
    24901 tgtttggcgc acagaaagac gatctgccgg agatctggcg tcagctgatt gaagccggct
    24961 tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgt gtgggtagca
    25021 cctggtgccg ctacggcgtt ggcgacagcg tcggcctcgg cgtggaactg gaaaaccgct
    25081 acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt acccgtgaat
    25141 gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc tggaacctgt
    25201 atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggcg gcggatatcg
    25261 atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc cgtactgccg
    25321 acaaactgac gcgtaccgca ccgtggttag aaaacctcga aggcggcatc gattacctga
    25381 aagcagtgat cattgacgac aaactggggc tgaacgcaca tctggaagaa gagatggcgc
    25441 gcctgcgtga agcggtactg tgtgagtgga ctgaaacggt caatacgccg tctgcgcaga
    25501 ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag atggtgccag
    25561 agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccagtaact ctggtggagg
    25621 acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc ctgaaaccgg
    25681 cgtctgcgcg ctgttaggtg acgagcaggt cgcgattttc cgcccgtatc acagcgatca
    25741 ggtgtttgcg atcagcaaca tcgacccgtt cttcgagtcc agcgtgctgt cacgcggact
    25801 gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac agcgttttcg
    25861 cttaagcgac ggcttgtgca tggaagacga acagttttcc gtcaaacatt acgaagcgcg
    25921 agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc gcaatgcctc
    25981 ccctttttgc atggtcctgt aatgaccttc ggtatattgc aggacatttt ttaaactttt
    26041 tgttttattt ttttgttttt attttttaaa ggataatcaa atgtttacag acactattaa
    26101 taagtgtgcg gctaacgctg cgcgcattgc acgcctgtcg gcaaataacc cgctcggctt
    26161 ttgggtcagc tccgccatgg cgggcgcgta tgtgggtctt gggatcatct tgattttcac
    26221 gctcggtaat ttgctcgacc cgtccgtacg tccgctggtg atgggcgcga cctttggtat
    26281 cgccttaacg ctggtgatta tcgccggttc tgaactgttc accggacaca ccatgttcct
    26341 cacctttggg gtaaaagcgg gcagcatcag ccacgggcaa atgtgggcaa tcctgccgca
    26401 aacctggctg ggtaacctgg tcggttccgt cttcgttgcc atgctctata gctggggcgg
    26461 cggtagcctg ctgccggtag ataccagcat cgttcactcc gtcgcgctgg ctaaaaccac
    26521 tgcaccggca atggtactct tcttcaaagg tgcattgtgt aactggctgg tttgcctggc
    26581 aatctggatg gcgctgcgca ctgaaggggc ggcgaaattt atcgctatct ggtggtgtct
    26641 gctggcattt atcgcgtccg gctacgagca ctctatcgct aacatgacgc tgttcgcgct
    26701 ctcctggttc ggcaaccaca gcgaagccta cacgctggcg ggtattggtc ataacctgct
    26761 gtgggtgacg ctgggtaata ctttatcagg tgccgtattc atgggattgg gttattggta
    26821 tgctacgccg aaagcgaatc gtccggttgc ggacaaattt aatcaaactg aaacggctgc
    26881 cggttaatta ctaaggggtt tttacgtgga tcatttgcct atattttgcc aattacgcga
    26941 tcgcgactgt ctgattgtcg gcggtggtga tgtcgcggaa cgcaaagcaa ggttgctgtt
    27001 agacgcaggc gctcgcttaa cggtgaatgc attagcgttt attccacagt tcaccgcatg
    27061 ggcagatgca ggcatgttaa ccctcgtcga agggccattt gatgaaagcc ttctcgacac
    27121 ctgctggctg gcgattgcag cgacggatga tgacgcgctt aaccagcgcg tcagcgaagc
    27181 cgctgaagct cgtcgcatct tctgtaacgt agtcgatgcg ccgaaagccg ccagctttat
    27241 tatgccgtcg attattgacc gctcaccgct catggtcgcg gtctcctctg gcggcacctc
    27301 tccggttctg gcacgcctgt tgcgcgaaaa actcgaatca ctgctgccgt tgcatctggg
    27361 ccaggtagcg aaatacgccg ggcaattacg cgggcgagtg aaacaacagt tcgccacgat
    27421 gggtgagcgt cgccgtttct gggagaaatt gttcgttaac gatcgcctgg cgcagtcgct
    27481 ggcaaacaac gatcagaaag ccattactga aaccaccgaa cagttaatca acgaaccgct
    27541 tgaccatcgc ggtgaagtgg tgctggttgg tgcaggtccg ggcgatgccg ggctgctgac
    27601 gctgaaagga ctgcaacaaa ttcagcaggc agatgtggtg gtctacgacc gtctggtttc
    27661 tgacgatatt atgaatctgg tacgccgcga tgctgatcgc gttttcgtcg gcaaacgcgc
    27721 gggataccac tgtgtaccgc aggaagagat taaccagatc ctgctgcggg aagcgcaaaa
    27781 aggcaaacgc gtggtgcggc tgaaaggtgg cgatccgttt atttttggcc gtggtggcga
    27841 agagctggaa acactgtgca acgcgggtat tccgttctcg gtggttccgg gtattaccgc
    27901 agcttctggt tgctctgcct attcgggtat tccactcacg catcgcgatt atgcccagag
    27961 cgtacgctta attaccggac acttaaaaac cggtggcgag ctggactggg aaaacctggc
    28021 ggcagaaaaa cagacgctgg tgttctatat ggggttgaat caggccgcga ctattcagca
    28081 aaagctgatt gaacacggta tgcctggcga aatgccggtg gcaattgtcg aaaacggaac
    28141 ggcagtcacg cagcgcgtga ttgacggtac gctcacgcaa ctcggtgaac ttgctcagca
    28201 aatgaacagt ccatcgctaa ttattattgg tcgggttgtt ggcctgcgcg ataaactgaa
    28261 ctggttctcc aaccattaat ttaacccggg ccagagaatt ctggccttct taatgactct
    28321 ttttatatat tcagcaaata aaacatagcc ccttaataaa tattatctgc taatgggtta
    28381 tattcaccgt tacagcgact tacaaaatta agtcattcgc tcactgacgc caattaaata
    28441 ttcctgtatc gttcttaacg agcagtctgc ttatattcat ataatcaatg aatattaatt
    28501 aatatataat acatagggaa tgtaaatgaa caaatttatt aaagttgcac tggtaggtgc
    28561 agtactggct acgttaactg catgtactgg tcatattgaa aaccgtgata agaactgctc
    28621 ttacgactac ctgctgcacc cggcaatttc tatttctaaa atcattggcg gttgcggtcc
    28681 tactgcacag taagtctctg acagaaatcg gctaacaccg gtttaattgc ccgacgccct
    28741 ctggattttc cgagggcgta tttgtttcta tgtccttctt gcccccgccc gtaaaaatat
    28801 tttcacttta aattcaattt gataactaca tccattagcc acagcgcgat cctgtgcgaa
    28861 attttgtgat cttcctccac attacataac atcatatgtt gttatattca tcatgcattg
    28921 tcatgttacc ttttaaatga ctgcaaactc tcccctacaa cgtattggac aagaaaaagg
    28981 tatcgctatg ggaagccagg aactccaacg caagctcgga ttttgggccg ttcttgcaat
    29041 cgccgtcggg acaaccgtcg gctccggtat ttttgtatct gtgggtgaag tggcaaaagc
    29101 agcgggcacg ccgtggctta cggtgctcgc gtttgtcatt ggcgggttaa ttgtgatccc
    29161 gcaaatgtgc gtctatgcgg aactatccac cgcttatccg gaaaatggcg cagattatgt
    29221 ttatctgaaa aatgccggaa gccgaccgct ggctttcctc tccggctggg ccagcttctg
    29281 ggccaacgat gcgccgtcat tgtcgattat ggcgctggcg attgtcagca atcttggctt
    29341 tttaacgcct atcgatccgt tgctcggtaa atttatcgcc gccggattaa ttatcgcctt
    29401 tatgttgcta cacctgcgct ccgttgaagg cggcgcagcg tttcagacgc taattaccat
    29461 cgccaaaatt atcccgttca ctatcgtcat tggccttggg atcttctggt ttaaagcgga
    29521 gaattttgcc gcccctacca ccactgcgat tggcgcaacg ggcagcttta tggcgctgct
    29581 ggcggggatc tctgccacca gttggtcgta taccggcatg gcctctatct gttatatgac
    29641 cggcgaaatt aaaaaccccg gaaaaaccat gccacgagcg ctgattggtt cctgtctgct
    29701 ggttctggtg ctctacaccc tgctggcgct ggtgatttcc ggcctgatgc ccttcgacaa
    29761 actcgccaat tctgaaacgc cgatttccga cgccctgacc tggatccccg cactcggcag
    29821 caccgctggg atctttgttg ccatcacggc gatgatcgtc attcttggtt cgctttccag
    29881 ctgcgtgatg taccagccgc ggctggaata cgcgatggcg aaagacaacc tgttctttaa
    29941 atgcttcggc catgtgcatc cgaaatacaa cacgccggat gtctccatca tcctgcaagg
    30001 ggcgctgggg atcttcttca tcttcgtttc cgatctcacc agcctgctgg gttatttcac
    30061 cctggtgatg tgtttcaaaa ataccctcac cttcggctcc atcatctggt gtcgtaaacg
    30121 cgacgattac aaaccgctgt ggcgtactcc ggctttcggg ctgatgacca ccctcgccat
    30181 tgcgtcaagc ctcattctgg tcgcctcaac ctttgtctgg gcaccgattc ccggccttat
    30241 ctgcgccgtc atcgttattg ctactggtct gcctgcttac gccttctggg cgaagcgtag
    30301 ccgccagctc aacgctttgt cgtaatgtta tctggagaaa ataaaatgtt ggatattgat
    30361 aaaagcaccg tggactttct ggtcaccgaa aatatggttc aggaagtgga aaaagttctc
    30421 agccatgacg ttccgctggt gcacgccatc gtggaagaga tggtgaagcg cgacattgat
    30481 cgtatttatt tcgttgcctg cggatcgcca ctcaacgcgg cgcaaacggc gaaacatctg
    30541 gcggatcgct tttccgatct tcaggtctac gccatttccg gctgggagtt ctgcgataac
    30601 accccgtatc gcctcgacga tcgttgcgca gtaattggcg tttctgacta cggtaaaacc
    30661 gaagaggtaa tcaaagcgct ggagctgggc cgggcctgcg gcgcactcac tgcggcgttc
    30721 accaaacgcg cggatagccc gattacctcg gcggcggaat ttagcattga ttatcaggcc
    30781 gactgtatct gggaaattca cctgctgctc tgctacagcg tggtgctgga gatgatcacc
    30841 cgcctcgcgc cgaacgcgga aatcggcaag atcaaaaacg atctcaagca gttgccgaat
    30901 gcgctcggtc atctggtacg cacctgggaa gaaaaaggcc gccagcttgg tgaactggcc
    30961 agccagtggc cgatgattta taccgttgct gcgggtccgc tgcgtccgct gggttacaaa
    31021 gaaggcattg taacgctgat ggaatttacc tggacgcacg gctgcgtgat tgagagcgga
    31081 gagttccgcc atggcccgct ggagattgtc gaaccgggcg ttccgttcct gttcctgctc
    31141 ggcaatgatg aaagtcgcca caccaccgaa cgcgccatta actttgttaa acagcgtact
    31201 gacaacgtga tcgtcatcga ttacgccgaa atttcgcaag ggctgcaccc gtggctggca
    31261 ccgttcctga tgttcgtgcc aatggagtgg ctctgctact acctgtctat ttacaaagat
    31321 cacaacccgg atgaacgccg ctattacggt ggtctggtgg aatattaatc cctctcccgg
    31381 cccggcaacg ggccggattt acgcaaggag ttacccgatg aaaacaggta tgtttacctg
    31441 cggccaccag cggctgccta ttgaacacgc atttcgtgat gcaagcgagc tgggttacga
    31501 cggcatcgaa atttggggcg gtcgcccgca cgcgttcgcg ccggacttaa aagcgggcgg
    31561 catcaaacaa atcaaggcgc tggcgcagac gtatcagatg ccgattatcg gctatacgcc
    31621 agaaaccaac ggctatccgt ataacatgat gctgggcgat gaacatatgc gtcgcgaaag
    31681 cctcgacatg atcaagctgg cgatggatat ggcaaaagag atgaacgcgg gttatacgct
    31741 gatttccgcg gcccacgcgg gctatctcac gccacctaat gttatctggg gacggctggc
    31801 agagaaccta agcgaactgt gtgagtacgc ggaaaacatc ggcatggatc taatcctcga
    31861 accgttaacg ccgtatgaat cgaacgtcgt gtgtaatgct aatgatgtgc ttcatgcgct
    31921 ggcgctggtg ccttcgccgc gcctgttcag catggtcgac atttgcgcgc cgtatgtcca
    31981 ggcggaaccg gtgatgagtt atttcgacaa actgggcgat aaattacgtc atctgcatat
    32041 tgtcgacagc gacggggcca gcgacacgca ttacattcct ggagaaggca aaatgccgct
    32101 gcgggaactg atgcgcgata ttattgagcg gggctatgag ggttactgta cggtggagct
    32161 ggtgacgatg tatatgaacg agcccagact ctatgcccgc caggcgctgg aacgctttcg
    32221 cgcgctgctg ccggaggatg agagatgaaa accctggcga caatcggcga taactgcgtc
    32281 gatatctacc cgcaactgaa taaagcgttt tctggcggta atgcggtcaa tgtggcggtg
    32341 tactgcactc gctacggcat acagccggga tgcattacct gggtgggtga cgatgactac
    32401 ggcacaaagc tgaagcagga tctcgcccgc atgggcgtcg atatcagcca tgtccatacg
    32461 aaacacggcg ttaccgcaca aactcaggtg gaactgcacg acaatgatcg cgtttttggc
    32521 gactacaccg aaggcgtgat ggccgacttt gccctgagtg aagaggatta cgcctggctg
    32581 gcgcagtatg acattgtgca cgcggcaatc tggggacatg cggaagacgc attcccacag
    32641 ctgcacgctg cgggcaaact taccgctttc gacttctccg acaagtggga cagcccgctc
    32701 tggcagacac tggtgccgca tctcgatttt gcctttgcct ccgcaccgca agaagacgaa
    32761 gcgctgcgtc tgaagatgaa agcgattgtt gcccgtggcg caggcgtggt gattgtcacg
    32821 ctgggtgaaa acggcagcat tgcctgggat ggcgcgcagt tctggcgtca ggctcctgaa
    32881 ccggtgacgg ttatcgacac catgggtgcc ggagattcgt tcattgccgg attcctttgc
    32941 ggctggtctg cggggatgac attaccgcag gcgatggcgc agggaacggc gtgcgcggcg
    33001 aaaaccattc agtaccacgg tgcctggtag gtataacgtt ggcgtgagca tcttcacgcc
    33061 aacgtgctgt tacttgccgg aaaacgaccc tataatccga gtaattcatt ctttatttca
    33121 gggtcgatta tgtcagctac ggaccgctac tctcatcaac tcctctacgc taccgtccgc
    33181 cagcggttgc tggatgatat cgcgcagggg gtttaccagg ccgggcaaca gatccctacc
    33241 gaaaacgagc tttgtacgca atataacgtc agccgcatta ctattcgcaa agctatcagc
    33301 gacttagtgg cagacggcgt actgatccgc tggcagggaa aaggcacctt tgtacaaagc
    33361 cagaaagttg aaaacgccct gcttactgtc agtggtttta ccgattttgg cgtctcacaa
    33421 ggcaaggcga cgaaagagaa agtgatcgaa caggaacggg tcagcgccgc gccgttttgc
    33481 gaaaagctga acatccccgg aaacagcgaa gtgttccatc tctgccgggt gatgtatctc
    33541 gataaagagc cgctgtttat tgatagttca tggatcccgc tgtcgcgtta tcctgacttt
    33601 gatgagattt acgtcgaagg aagctccacc tatcagttat ttcaggagcg ttttgacacg
    33661 cgagtggtca gcgacaaaaa gaccatcgat atctttgccg ccacccgccc gcaggcaaaa
    33721 tggctgaaat gcgaactggg cgaaccgttg tttcgcatca gcaaaatcgc ctttgatcag
    33781 aatgacaaac cggtgcacgt ctccgaactc ttctgccgcg ccaatcgcat caccttaact
    33841 attgataata aaagacatta accgtaggcc ggataagatg cgccagcatc gcatccggcg
    33901 atgctggcgc gttgaatttt acatcccgta cgttcccctc accctaaccc tctccccaaa
    33961 ggggcgaggg gaccgttcag tacggaattt agcttgacgg tgctatccag cattaaatac
    34021 tggcaatact ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa
    34081 tgcgaatcgc acaatgttct gattgtggat tcgcctggcg aaacgttccg gaaaggcgat
    34141 aaaagagcgg cgggatttca tatttcgact ctgcacccac cgggtaaggc aaggcaccgc
    34201 gcttttgcgc ctcttccagc actctggcgg caatcggctg atgaaactcg acaatcaaca
    34261 ccttcgactg cgcattagca atcaccgcgc ttttcacttc cggcaccgca ccaccgttaa
    34321 gcaaagccag caaccgttca gatacccctg cctgcaccgc gtgcatcact ggcgcaaaca
    34381 ccagaccacg caatacttcc agcgcctgtg cgccctggat ctggctaccg ccggagtaaa
    34441 gcgtggcgcg aatacggttg ataacatcag catcgccgac cactgcacca acaccctctg
    34501 gcccaaatag cttgaagcag gaaaatgtcg agacattcgc gccgcattca cagccgattc
    34561 gagccacctt catcaccgca tagttgtcat cggttaacgc tggaacacct gccgcgcgca
    34621 acgttgccag cacatctgcc agcacgtagc tgtcctgcgg ctgctggcgc gtatgctgca
    34681 ccagcgccgc atccggttgt tgctcgtcga cgacctgctt cagtgccgac aggtcattga
    34741 aatcaacagt aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa
    34801 caggcgcgtc atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc
    34861 gaatcgcgcc agtccccgcg ccctgcacca gcgccgcagc ctgtgcgtga aatgcatcag
    34921 ccagcacctg ttccacacgc tgggtaacgc gcggttgatt cagtcctggc gttaagccta
    34981 aatcaccgcc agtaagaaac tcgctgccgg ggaaatggcg acaaatgcta tccaccagcg
    35041 caaactgctt ttgctgcgcc tcaataatcg tcaggctttg cagaggaaac gtcttcatag
    35101 gtgccttcct taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc
    35161 ggtgatcatt actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc
    35221 gttgaggaag taaatggcaa ccgcgataga gaatccggta taacccgcca tcttaattgc
    35281 tgcgaaaatc gaaccgacca gcagcgccac ttccatcagc atattcatgg cgttacggat
    35341 gttatccgac gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag
    35401 cagcaagact tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg
    35461 cgagagatag cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc
    35521 taacgcggtg gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc
    35581 cgcctgatta atcagcgttt gcgattgttc cggcgttacg cctgcggaat atgctttctc
    35641 cagtgtgaag atcgacactt cactgccagc aaaaatcttc atgctggcaa cggcggcaat
    35701 caatgctccc acgatggcga tatagggtaa gtttttgata atccgtgacg tgcgttcttc
    35761 aaacaccgaa agcccgctgg catcaatgtc attttcatca cgatgacgca ggtcgtgagt
    35821 tatcgcgatc cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag
    35881 atgtgggaaa tagcgcacga ctaccacacg ggtcatcagt accaccacgg cggcgatcag
    35941 actttgcttc cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagctga
    36001 aaccaccggc gagcttaatt cccccaggct acctaataca tccaccggca gcgcggtcag
    36061 cagctggttt actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc
    36121 aaacgccatc aggctgttta tcgccaggac gccgaggata tcggtaggaa gaaagagtaa
    36181 ccatgcgttg agcagcccgg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc
    36241 aatgctcaga ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag
    36301 ttgcggcagg atcgggcgga tgccgtcatg aaaaacagcc gccgagcgat gcgccagaag
    36361 cgatgtcata cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt
    36421 gccttttatt tcagatggtt aatcagcatc ggaatcgcgt gttcgacgtg ctcaacggaa
    36481 aggccaaacg ccacttttcc ttcagcgatc attttggcga tatgctcgtc tttcgcttta
    36541 atgcctggtt tggcaatggt gcaacttttg ttatagccta tcaccgcgat ggcaatcgac
    36601 aatgccgcgc ccgcaccggt attacacgcg ccgatgtaat aatccagttg cccggatttc
    36661 accttcattg ccgcttccat gtcgttgtga atgaaaactt caaaacagcc aggagccgtc
    36721 gcttcaatag tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg
    36781 agtctgtcct tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc
    36841 ctgactgaat cctgactggc gcaattgcgg aataaaggtg gttagtaaat agtcatagcc
    36901 ataaccaccg ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg
    36961 gttcagcaac ccacggtcgc gtagcgcatg aagcatcgca atacgctttt cgtccgggta
    37021 gtaactgttc ttgccgatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa
    37081 aatgttgtcg aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac
    37141 cccgtgggct tgtagcaacg ccagttgctc cagccccatc gtgctgaacg acgtatgcgt
    37201 ggagatcggg cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc
    37261 cagcggcgta atctttcctt cgctggtgcc gatctccgcg atgatcccgg ctttcagctc
    37321 cgtgccatcg ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct
    37381 gcgggtcgcc acatgttccg ggaaaaacgc gtcctggtaa taaccggtac aggccaccac
    37441 gttgatcccc gtctcgcgca ttacatcaag cataaattgc gcattgcgcc ccatgtaacg
    37501 gttggtcatc tcaatcacat tacgcacgcc ccgggtcatc aggtcgttca tctcctggca
    37561 aatgaacgca tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat
    37621 atgcagatgc tcatgggcga gggtgtaacc cgtcggatca aaactcatag ggtgtctcct
    37681 gcaaaccgga gggaggaaag aaaagagcga ccattttgtg gcggtggcgc gcggaaaaat
    37741 tcacacacgg tagcccccac atcagaaagc gtggtgcgca caccgagctg cgtagcgatc
    37801 atcccttgct gataaaccag cactggcacc acttcgcggg tatggtggct gtgaccaatg
    37861 gtcggatcgt tgccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca
    37921 acaagccggg caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct
    37981 tctgcatgac cagcgaggtc ggtttcctga atgttggtgc aaataaacgc cgtcggatgg
    38041 gtgttaaatt cgttgagggt gatatccata atccgctggc tatccaccag attttgccag
    38101 ctcacgccat aaggattgtt gacgatatct gccaccttac ccaccagcac ggttggcacg
    38161 cctgcttcat acagtttttg tggcacctgc actttttcat cgacgccata gcccatatgc
    38221 acgacctgga aaccgttgtc ataagcgcca gaacgcggcg cattgatacc aataaagcgc
    38281 ccttctttgc tttctgcggc atcgagaatg cgttgactgt cggttaacag gccaccaaat
    38341 gtaatgaccc gaccgacctg tacctgctca cgcacgatac gaccaatttt gattgcgtcg
    38401 tcaaaagaga tcacagagag attggcggta atgttataga cctggcctaa atccgcctcg
    38461 agattatcgc caatcgcaac cgcctgattg acccacagaa attgcagatc atcgccacgg
    38521 cgctccacct gccagccagc ggaaactaat gcctgctcaa cacggtcaat cacatcgcga
    38581 aaaggcatcc gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag
    38641 gtatcgccac cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc
    38701 atatcgcctg gcgcataacc caatgcgttg attagcccca gcttctccag cgttggtagc
    38761 tgcaaatgcg gcaactggct caggatgtga ccacaggtat tcgctcccgc atcttgcgga
    38821 cgcaccagcg tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca
    38881 aatcgcgcca ttacgccccc agagagtttc caagactgtc ataacgacca acgattttcg
    38941 gttcgccacg gtgaataccg gacaccagca ccacatcgct acgggtgaca aaaatctgcg
    39001 tgcggaaaca gagcaccact gcactgctca ccggatactc gcctgccagc ggcagggtgt
    39061 agtcgatact gctgtcatcc acagttttga gattggtttc agtaatcttt tgattttctg
    39121 gcgtaaacac cagcgcatgt tgcgcatgac cacgacggta gtaaccgccg ccgtagcagt
    39181 agctgtcgcc acggaaatga tgggagattt cacttaacca gagcatcgcg atacgttcag
    39241 gctgatcgcc ctgctggttt gccggaatag tgcccgtcag cgcatgaccg ggttcggcat
    39301 gagtcacacc gtattccgcc agcaatggca gcgaagtgca gctggtcgct gaaggcgcgt
    39361 tcagttgctc aagtgcaata ccagatttcg ccagttgatc ccgtgcctgt atcagcgtgt
    39421 gaagattcgg tgtcggcaaa acttttccgg cagcctcatc ccaaagcagg caagggaaat
    39481 gggtaagtcc ggctaaatgc agccccggca gtttcttaat ttcagcaacc acctcgtgca
    39541 gcgaatgctg tacaaaaccg ctctcctgcc ccggataaag aaaatcatcg tcgctataaa
    39601 ctttgagcag cacaaactgc acacgtccgg ccttcaccgc tgccgcagaa acttcccgcg
    39661 ctttgtcgag agtaaacacg gtgatgacgt cggtgccctg ttcaacggcg tcagcaacct
    39721 gatgacaagg gatttgtacc agatgcccct gatgcgccac cgacaaacca gcgcggcgca
    39781 tgactcgcgc ctctttgtaa tccaccgcca caatgccgct gtaacctaat gccagcaatt
    39841 tttccgccag ccatgggtta cggccaaact gcttggtcat cagatacagt tcaatgccgt
    39901 aaagccgcgc cgtctcaatc agtcgcttac cgttttccag tacctgatcc acgtcgatca
    39961 cccagctgtc cggggcgatc ttgccctgct gccacaaact tagtgcggcg gaaatcagcg
    40021 ccgggttctg gcgtttcaat gcttctacaa acatcttgtt gcctcgtttc tgttccttga
    40081 attaaatatt catttttttg aatatttagg ttgtgcgacg tggatttgtc gggagcttca
    40141 aacctcttcg ttagcagcca tccataatcc ataaaggttg gccagcaaat agccttcttc
    40201 acacgggtta acttccagtg cgaattcctt caacaacacc tgatgcaatt gcacaacggc
    40261 ttgccagtgg ctggattgcg ccagttctgc cagcaactcg ttatccagcg gctctatttc
    40321 ttcaccacgg cgactgcgca tcagtgcact cgccatatgt gtcatcgcca tcgttccttg
    40381 ctcactgcgc accggcagat ggcactctgt ttccagtacg ttgacgacct gcatcatgcc
    40441 tttgcagatg tccttatcaa taacgcctgc ctcgcaaagc aggttgagtc tggtttccat
    40501 atcagatgcc tttcgtttaa taactcggtt cctgttcccc actcactacc cgttgttgat
    40561 gggcgagcag ggcgtgttta tctacaacgg cacgcagcag ttgttgcatt gggtaatcat
    40621 cgactcgcgt caggaccacg gcttcggtgg cctgtaaaaa tcgcggatcg tctgtgagcg
    40681 gcgtcgcctc taatcccagc atggtcagtt cgttttccgc caccacgttc cagatcaccg
    40741 catcgacatc gcctttaaca atgcgttgta aactctcgtg ataagagaga tcgactcgtt
    40801 ccacatcact accgccaaaa aaaacatcgg tcatgatttt ctgatccgcc gaacggttat
    40861 ccagccccac gcgcttcacg tttgcggact cgcctttacg gcaaatcaac tggtgctcgc
    40921 caacgtaggt gtgcggcccc aactccagcg cgaggcataa gcctttttgc gtgagataac
    40981 tttccgccgc tagtcgcgaa accaccgcca tgtcatacac gccattaagc agacactcca
    41041 cgcgaatatc cgcgccacgc atgtgcgcat agtaaaaagg aatgccatca aactgggctt
    41101 tcaatccgct cgccaggcct tcgtacaaac gggtataggg caagggcatt gcacatacca
    41161 cgttgttgat atccacatga gtcagcaatg ctttgttatc catctcgacc agataactgc
    41221 cattgcgccc acggcgttca atccgtatcg ctccgcttga ttccagcgtt ttcaacgcgg
    41281 cctgcgtcag gccaacggat gaacggcatt catttgccag ctcatctatg gttttcaatc
    41341 gattaccgca cttttcaccc aacaaataac gggccagcgt cgtgatgacg acgccttctt
    41401 ttttgataaa cgttcgacgc ataataaatt ttcagtaaat tgaatattta tatcttcagg
    41461 aatttgaaga taaggcgcaa cagcgagatg tggaaacggc gaggcacttc acattttttc
    41521 ggattattgc aaatgaggat aaaaaaaccg ggtttccccg gtttcagagt gatgataaaa
    41581 gcaaaattgc ctgatgcgct acgcttatca ggcctacatt tccttgcaat atgtgcatta
    41641 ctttgtaggc cggataaggc gttcacgccg catccggcat gaacaaagcg caatttgcca
    41701 gcaatagtga attacggctt cgccacaaaa ccaatcgctt cgtacaccgc ttttagcgta
    41761 cgggaagcgt gcgcgctggc tttttccgcg ccatctttca tcacctgttg caggaaggct
    41821 tcatcgttgc ggaaacggtg atagcgttcc tgtaattcag tcagcatacc ggaaacggca
    41881 tcagccactt cacctttcag atgaccatac atcttgcctt cgaactgttt ttccagttct
    41941 gggatgctct ggcccgttac cgctgaaagg atatccaaca ggttggaaac gcccgctttg
    42001 ttctgcacat cgtagcgaac taccggcggc tcgtcggagt cagtgaccgc acgtttgatt
    42061 ttcttcacta ccgatttcgg atcttccagc aggccgataa cgttattgcg attatcgtca
    42121 gacttggaca tcttcttggt cggctccagc agcgacatta cgcgcgcgcc agatttcgga
    42181 ataaacggct ccggcacctt aaagatctcg ccatacagcg cgttgaaacg ctgggcaata
    42241 tcgcggctca gttcgaggtg ctgtttctgg tcttcaccca ccggtaccag attagtttga
    42301 tacagcagga tgtccgctgc catcagcacc ggatagtcaa acagaccagc gttgatgttc
    42361 tcggcataac gcgcagattt atctttaaac tgcgtcatgc gactcagttc gccgaagtag
    42421 gtatagcagt tcagtgccca gcctaactgt gcatgttccg gcacgtggga ctgaacaaaa
    42481 atggtgcttt tctcaggatc gataccacaa gccagataca aggccagcgt atccagcgtc
    42541 gctttacgca gcttctgtgc atcctggcgc acggtgatcg cgtgttggtc aacgatacag
    42601 taaatgcaat ggtagtcatc ctgcatgttt acccactgac gcagcgcacc catgtagtta
    42661 ccaatggtca attcacctga gggctgtgcg ccactaaaaa cgatgggctt agtcattttt
    42721 cgattcctga ttttcgctat gcggaagccc taatgcgggc agaaggtcat ttatagactg
    42781 ataaattaca tcaggctggc tgagatcgat agcctcgccg tagttatatc cgtaggttaa
    42841 gccaactgat gggcaacctg ccgcttttgc cgcctgaata tcattgcgtg agtcgccgac
    42901 aaacagcatc tgttgtgggg caattcccat ccgctcagcc accagtaaca gcgggtccgg
    42961 atgcggtttt ttgttttgca catcatcacc accaatcacc acgctgaagt atttggcgat
    43021 atctaaggct tcgagcagcg gcgcgacgaa cggcgtcggt ttgttggtga ccaggcctag
    43081 cggcaggcct ttagcctgca acgcgcccaa cgtatcggca acgtgcggga acaaaaacgt
    43141 cccctcttcg gcaacctcgc catagtagcg atcgaacagt ttacgcagaa tacgtacctg
    43201 ttcttctgcc ggaatgtcgt catcaacggg cggtttaccc attgttttac gctgagtcgc
    43261 acgttcctga cgcgcccagg tcaatgcgcg ctccatcaga acatctgcgc cgttaccaat
    43321 ccaggtaata acgcgttctt cacctgcgac gggcaactcc agcgcataca gcgccatatc
    43381 taccgcagca gcaagaccag gagcactgtc gaccagcgta ccatcaagat caaaagcgac
    43441 gccgcgaata tcttcaaact tattcatgac ttacctttgc cagttcactg cgcatttcat
    43501 caatgacttt tttgtagtct ggctggtcga agattgccga accggcgacg aacatatccg
    43561 cgcccgccgc agcgatttcg ccaatgttgt tcaccttcac gccaccgtcc acttctagtc
    43621 gaatgtcaaa gccagactcg tcgatacggc gacgtacttc gcgcagttta tccagtgttt
    43681 gaggaatgaa agactgaccg ccgaaaccag ggttgacgga catcagcagg atcacatcca
    43741 gcttatccat cacgtaatcc agatagctca gaggtgtcgc cgggttaaat accagacccg
    43801 ctttacagcc attttcttta atcagttgca gcgtgcggtc aacatgctcg gaggcttctg
    43861 gatgaaaggt aatgatgctg gcaccagcgg cagcgaaatc aggcacaatg cgatcgacgg
    43921 gtttcaccat caggtgtacg tcgatagggg cggtaatgcc atagttacgc aaggatttca
    43981 gcaccattgg cccaatcgtc agattgggaa catagtggtt atccatgacg tcaaaatgca
    44041 cgacatcagc gccagctgcc agggcttttg cggtatcttc acccaggcgg gcaaaatcag
    44101 ccgacagaat tgagggggca atcaaatact gtttcatccg cttctccttg agaattattt
    44161 tttcgcgggt gaaacgactc ctggtttgta caaagccagc agttcgtcca cctttttacg
    44221 tgtgccgccg ttgctgctta tactgcgtcg aactttgacg acatgcaatt ttgcgcgctg
    44281 ataccactca cgcgttaaca tcgtatcgtg attggagatc agcactggaa tatggcgctc
    44341 aaccagacct tcggcgatct ccgccagatg cgcttgttgt tcaagcgtaa aactgtttgt
    44401 gtgatacgcc gtaaagttgg cggtcgcaga cagcggtgca taaggcggat cgcaatagac
    44461 gacggatgca tcatctgcgc gcgccatgct atcggcgtaa gactcacaat agaaaaaggc
    44521 attctgcgct ttttcagcga agtgatacaa ctctgcttcc gggaaatagg gttttttgta
    44581 gcggccgaac ggcacgttaa actcaccgcg cagattgtaa cgacacaggc cgttgtaacc
    44641 gtagcggttc aaatataaaa acagtaccgc ccgacggaac ggatcctggc ttttgttgaa
    44701 ctcttcgcgg aactgatagt aaacctcggc gcaatttgtt tcgggaacaa acagctcgcg
    44761 tgcggcctgt acgtactcat cagtacgcat cttcacaatg ttatagagac tgatcaggtc
    44821 gctattgata tcggcaagga tataacgaga aaagtcggtg ttgagaaaca ccgacccggc
    44881 acctacaaaa ggctcaacca gacattcgcc cttgggcaaa tgccgtttaa tatcatcaag
    44941 caggggatac ttgccccctg cccacttcaa aaaagcgcga tttttcttca tgctgactaa
    45001 ctaattacac cttctccggc tgtggagaaa gctccgacag catccagcgc ttcaggcaac
    45061 tcccgcaacg ataaaccatt gcgggagata accttgatta cttcagatcg gcctgtacct
    45121 gacgcagcgg tttcgcccac gggtttttgg cctggacatc tgctggcaat gtagataccg
    45181 cttttttcgc ctcttctttc gaagcgtaca cgccagaaac caggacatac cacggctgac
    45241 cattacgcgt cgtttcatag acaacgtagt ttttcagatt ctctttcttc gcccaaccgt
    45301 tcaggttgtc gtagttagag gaactgctca gctgcagagt gtaatggctg gacggtgccg
    45361 atttcaacga accaacatta cctgcggtct tcgccccagc agcgggtgtt gctgtggttt
    45421 gcgccgggga tgccgtctgt actggagccg tagtcgccgt ctcttttggt gctggcgtag
    45481 aagtcgcagc cggtgccttc gtgctcgcca caggggcagc tggttcagta cgcttcggcg
    45541 tctgtgctac cggcttcggc tccgttttca cggttgcttg cgattttttc ggttcaatca
    45601 ctgcctgctg gcgagctgga cgcgtagtgg ccggacgttc agcggtttgc gttttcgccg
    45661 tttcacgcga tgcattgcca ttgcgaacag gcgcgacagt cgcgggttcg gtcggcaatg
    45721 tggaattgac cgccacattg ttcaactgtt gctgattttg tggctgggtc agcgcattgt
    45781 tcaggtcacc ctgcacttca acacgttgtt gaccatccgt tgccgccggg gtttgccctt
    45841 gagtcggcgt agaagagatc ggtggcagag aaacatcctg ctgagtattt tccgcagacg
    45901 tggttcccgg cgctggctgc accccattcg cctgatcggt cgcattgcct gcaagatcaa
    45961 tactcttctc accagacgcg gtttgatcgc tggaagaggt cgagggggct tttagcgcag
    46021 aaccgatacc gatgatcaac agcagtagaa ccagaatgcc gacgcccatc atcatatact
    46081 gacgagaagc gggtttactg gctgcttttt tgcgcttacg cggacgacgc tctacgcgct
    46141 cttcatccac ggtttcatct tcggattcgt caatttcttc ttcgatttcc ggttcctcat
    46201 tgcgctcttt ttgcgcacga gtcggacggc gatcgtcagt gtcatcaagt tcaatatcat
    46261 caaaattgat ctgcggttcg ccacgttcag tacgctcaga acgttcagaa gattgacgag
    46321 aacgaccagt acgacgatcg ctgggatcgg gtttcagctc gtcttctggt ttgaattcat
    46381 ccatttaaca ccccactaaa aggttaatgc ttaccacgtt gcaattaacc tgaagctaat
    46441 agaccgcttg ataagcggcc tgacctttct tgttgttacg ctgattgaca atcggcaatg
    46501 gcgttaagaa caagctcgtg cgaaacgccg ctgcgaactt cactcttacc aattgccaac
    46561 ggaagaatta agcgcatctc tcccgcaagg actttcttgt cacgcagcat atgcggtaaa
    46621 tacgcctgcg cggacatttc gcgcggccca ttgaccggta acccagcccg cgtgagcagg
    46681 gttataatac gctgcgtttc ggcagaacta aactgcccga gacgttccga cgtccgcgcc
    46741 gccatcacca tacccgcagc gaccgcttca ccatgtaacc aattgccata ccccatttca
    46801 gcttcaatgg catgaccaaa ggtgtgtccc agattcagta aagcacgtaa cccggtttcg
    46861 cgctcgtcgg cggcgacaac ttctgccttc agttcacaac aacggcgaat acagtacgcc
    46921 attgccggac cgtccagacg caacaacgca tccagattct cttccagcca gttaaaaaac
    46981 gcaccgtcaa gaataatgcc gtatttgatg acttctgcca gccccgacgc taactcacgc
    47041 gggggaagcg ttttcagaca gtcgagatcc accaccactg aagcaggctg gtagaacgcg
    47101 ccaatcatgt ttttaccgag gggatggttg accgcagttt tgccgccaac ggaggaatcg
    47161 acctgcgaca gtaacgtcgt cgggacttga atgaaacgaa caccgcgctg ataactcgcc
    47221 gccgcgaagc cggtcagatc gcccactacg ccgccgccaa gcgccaccag cgtagtatcg
    47281 cgaccatgcg gcttttgtaa caacgccgta aagacggtat cgagtacagc caggctttta
    47341 tactgctcgc cgtcagggag gataacgcta tcgacgttaa cacccgcctg ttcaagtacg
    47401 ccgcggacct tatcgagata cagaggagcc agggtttcgt tggtgaccaa catgacctgc
    47461 tcgcccgatt tcagcggtaa gaatgaagct ggttcattaa acaaaccaga tgcgatggta
    47521 attgggtaac tacgttcccc gagagtaacg acaatcctct ccataacgcg acatccacct
    47581 taattactgt acccgcagac gagtgtatat aaagccagaa ttagttgctt tccagcatgt
    47641 gaataatctg gtttgcaacc actttagcgc tttgatcatc agtacgaatg gtcacgtcgg
    47701 caatctcttc atacagcgga ttgcgttcat tggccaacgc ttccagaact tcacgcggcg
    47761 gtgtttcaac gtgcagcaac gggcgttttt tatcacgctg cgtgcgtgca agttgctttt
    47821 cgatggtcgt ttcaagataa acgacaacgc cacgagcgga aagacggtta cgcgtttcac
    47881 gggatttcac agagccgccg ccagtagcca gcacaatacc ctgtttctcg gtcaactcat
    47941 tgatgacctt ttcttcgcga tcgcggaagc cttcttcgcc ttctaaatcg aaaacccagc
    48001 ccacatcagc tccggttcgt ttctcaatct cttgatcgga atcgtaaaat tccatattga
    48061 gttgttgagc taactggcgc ccaatagtgc tttttccggc acccataggc ccaaccagaa
    48121 agatattgcg tttctctgcc attttttcgg tactactaag actattcgtt aatgataaac
    48181 ccgcttcgct cagagagcgc cgcaggacat gaactgaaac ctcataagat attgcgagag
    48241 tcagactgaa aattatctca atactccagc gggtttggca actgaataaa tcacccatcc
    48301 ctctgcatat ctggtcgctg caagcgcgct gccttgctac caccgctctg gcgataaatc
    48361 accgggtaag attagcgtaa aaaagacagc aaaatgccgc ctgaatgata aatcatcatc
    48421 atggaacgca ggcagcgaag agcaccaact ctcaaatcgg tactccttgt atgctaaata
    48481 cctgcgcgcg tcaaatagat gaaacacgtt cagcataaaa acattaccgc ttttacggct
    48541 gtttactcat tggaaaccag tcgtggcgtg ataaacacca ctaactcgcg tcgttcatct
    48601 tcttttccgt catgacgaaa taattgcccg aaccagggaa tgtcgccaag caacggtacg
    48661 ctatcctgac ccgatttatt tttacgggta aaaatgccgc ccagcgccaa cgtttctccg
    48721 cttttgacct cgacctgcgt ttcgatctcc tgcttatcaa tcgccagcac ttcgccatcg
    48781 gcctgctgta gcacctgccc cggaacgttc tggctgatgt gtaatttcag ccggatgcgg
    48841 cctttttgta acaccgtggg cgtgacctcc atccccagga cggcctcttt aaattccacc
    48901 gacgtcgcgc cactttcccc gctggaaacc tgatatggaa tttcgctccc ctgtttaatg
    48961 ctggcaggct gaagatgtga ggccagcaga cgcggactgg cgataatatc cagttgctgt
    49021 ttttgttcga gcgcggaaag ctcaagatcc agcaagcgtc cgttgatgcg cccaatgtta
    49081 aaaccgacat gcgttgtcgc cgtcgctacg gagaggtcgc taccaagcgt ggtgacttgc
    49141 ccaacgccac cagcgtgttg cgcatcggcc agcgtccatt tcacgcctaa ctcacgcaaa
    49201 cttttttcat taatggtgac aatatgcgcc gacagctcaa cctgcccgac cggcagatcc
    49261 atttgcgcta cccactgttc aagcgcgctt aacgccgttt tgttatctcg tagcaaaagg
    49321 cgattggtgc gtttatcgac ggtcatactc cctttggcac tcagtagctt ctcccccgct
    49381 ttcgccagtt ctcccgcgtc ggcgtattgc agggttatac tgcgattttc cagcggcaga
    49441 tttgcctgcg cccgcgcctg ctccgcctcc tggcgggcga tattgttatt ctgccaggca
    49501 atggaatgca ctgagagaat gttgccttcc tgccgcgtta tcagtccggc gcttttcact
    49561 acagtttgta gtgcctgctt ccagggaaca tctgttagat gtaacgacac cgtaccgctg
    49621 acgtctggcg acacgaccag gttcaacttc tcctgttcag ccagcgcctg caacacctga
    49681 gctaccggaa cgtcatccac catcagcgtc actttttgcg gctttgccgc ctgtacgccg
    49741 ggtatcagca tcaacagtag tgcggctatc cattgcttca tttgtatctc cttgccgttg
    49801 ccacaaccat tgtggcggtt cgcagtttgt cccggtaccc agcgttagta cgtctggcgt
    49861 cagctgtaaa attgtccagc cgttttccag cacatcgttt tgctgcaccc gtcgccattt
    49921 cttttgcccg tcttttatta caccgatgat gcgctcgcct cgccctacca tcccctgata
    49981 gcgccactgg ctaagttcgc taatccggca tagatcttcc ggcggtttaa aagggtcacg
    50041 cataccggtt aaaaggcaca atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg
    50101 gcgtctccag ttgtagcgtg aacaaaagat catcaccttc cacgcttaac gaaaaacggc
    50161 tcacgctgac gttgcgctct gccagccgtg taaatgccga cggcactgct tcccacagcg
    50221 ttttcaacgc caactcgcct ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg
    50281 ataactgaaa atccagtggc gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt
    50341 ctaccaggcg atacagtgcg gcccactgct ggtgatgact tgcccgtagt cgaattaatg
    50401 cgtcgcgctc ttcatggtgt gtcgatgaca gaaaaatgag cgtaactaac atcagcaatc
    50461 agaatgccca gcaaagctgg cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca
    50521 tgttcatcgc taaccttcct tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc
    50581 tgctgcgtgg ctccccgctg attgagatga aaagatgcat cctggcggag tgacgtttct
    50641 agtgcgttta acgcggtaat gcttgttgtc agccccttga tctccagcgt tccctgctgc
    50701 cagcttatcg ttgtcagcca ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca
    50761 gattgccagt cgcgggtaaa ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac
    50821 tgctgttgct ccagcaaacg tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc
    50881 gcctgaagca aaactgcgtc tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc
    50941 gttatcccga cggccagcag cagaggcgca acgaacatca gcaaccagaa acgcagaaaa
    51001 gcggtccggc gttgctgtcg ccagggcaaa aaattaattg gcgggttcat cagtacgcct
    51061 tcccaagcgc cagccccagc gcgatggcaa agtctccacc tggcggcggt agcggcggct
    51121 gacgaacaga aacggcctcc cagggatcaa atccgccttc gccacatatc gcgacgcttt
    51181 cgggatcaac ggataacgct gccgccagct ccttcgcgct agtcatcccc accgccagtt
    51241 tgcgccccca gctatagcgc gtcgcccaca gccactgttc gttatcacgc caggccagac
    51301 attgctgatg agaaggtaaa aaaggcagga atcgctgtaa tgcactggca tccggggtga
    51361 tcgcgctcac atgaacacgc aacctttctg ccagagtaag cagcgttgcc agctctttgc
    51421 tttgcgcggc agtcacgtta taggcgggac tgagtgagtc ttcgctgtaa tcgaagcgca
    51481 gggagtccgg atccatatcc agctcgcggg ccatcgtccc tgacagccag gccgtttgct
    51541 cccgctcacc aagggacatc gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg
    51601 gaaacgccaa cataatgtga tgacgctgcg gcagttcgcg actccacggt aacaacgttt
    51661 tagccagctg ctgcgcatca acaatccgcc catctttgat aatgtcgttc tccagcggca
    51721 accgccacca gcgttgcaaa aagcattctt ttgcgccccg cacgatcgca accgctaccg
    51781 cttcttgctg ttgtaaatgc aaaccaattt gccagatctt aaatgccatt gtgatgatct
    51841 ccttatcacc cgtcactctg acgggtatat caatgcgtct ggcttgcctt tatactaccg
    51901 cgcgtttgtt tataaactgc ccaaatgaaa ctaaatggga aatttccagt gaagttcgta
    51961 aagtattttt tgatccttgc agtctgttgc attctgctgg gagcaggctc gatttatggc
    52021 ctataccgct acatcgagcc acaactgccg gatgtggcga cattaaaaga tgttcgcctg
    52081 caaattccga tgcagattta cagcgccgat ggcgagctga ttgctcaata cggtgagaaa
    52141 cgtcgtattc cggttacgtt ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg
    52201 acagaagaca gccgcttcta cgagcatcac ggcgttgacc cggtggggat cttccgtgca
    52261 gcaagcgtgg cgctgttctc cggtcacgcg tcacaagggg caagtaccat tacccagcag
    52321 ctggcgagaa acttcttcct cagtccagaa cgcacgctga tgcgtaagat taaggaagtc
    52381 ttcctcgcga ttcgcattga acagctgctg acgaaagacg agatcctcga gctttatctg
    52441 aacaagattt accttggtta ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc
    52501 ggaaaaacgg tcgaccaact gacgctgaac gaaatggcgg tgatagccgg gctgccgaaa
    52561 gcgccttcca ccttcaaccc gctctactcg atggatcgtg ccgtcgcgcg gcgtaacgtc
    52621 gtgctgtcgc ggatgctgga tgaagggtat atcacccaac aacagttcga tcagacacgc
    52681 actgaggcga ttaacgctaa ctatcacgcg ccggagattg ctttctctgc gccgtacctg
    52741 agcgaaatgg tgcgccagga gatgtataac cgttatggcg aaagtgccta tgaagacggt
    52801 tatcgcattt acaccaccat cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt
    52861 aataacgtgc tggactacga catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg
    52921 aaagtgggcg agtcggcgtg ggataacaac aagattaccg atacgctgaa ggcgctgccg
    52981 acctatggtc cgctgctgcc tgccgcagtc accagcgcca atcctcagga agcgacggca
    53041 atgctggcgg acgggtcgac tgtcgcattg agtatggacg gcgttcgctg ggcgcgtcct
    53101 taccgttcgg atactcagca gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg
    53161 ggacagcaaa tctgggttcg tcaggtcggc gatgcatggt ggctggcaca agtgccggaa
    53221 gtgaactcgg cgctggtgtc gatcaatccg caaaatggtg ccgttatggc gctggtcggt
    53281 ggctttgatt tcaatcagag caagtttaac cgcgccaccc aggcactgcg tcaggtgggt
    53341 tccaacatca aaccgttcct ctacaccgcg gcgatggata aaggtctgac gctggcaagt
    53401 atgttgaacg atgtgccaat ttcccgctgg gatgcaggtg ccggttctga ctggcagccg
    53461 aagaactcgc cgccacaata tgccggtcca attcgcttac gtcaggggct gggtcagtcg
    53521 aaaaacgtgg tgatggtacg cgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat
    53581 ctgcaacgct tcggcttccc ggcacaaaac attgtccaca ccgaatcgct ggcgctgggt
    53641 tcagcgtcct tcaccccaat gcaggtggcg cgcggctacg cggtcatggc gaacggcggc
    53701 ttcctggtgg acccgtggtt tatcagcaaa attgaaaacg atcagggcgg cgtgattttc
    53761 gaagcgaaac cgaaagtagc ctgcccggaa tgcgatattc cggtgattta cggtgatacg
    53821 cagaaatcga acgtgctgga aaataacgat gttgaagatg tcgctatctc ccgcgagcag
    53881 cagaatgttt ctgtaccaat gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag
    53941 actggcgcgc aggagtacgc accgcacgtc atcaacactc cgctggcatt cctgattaag
    54001 agtgctttga acaccaatat ctttggtgag ccaggctggc agggtactgg ctggcgtgca
    54061 ggtcgtgatt tgcagcgtcg cgatatcggc gggaaaaccg ggaccactaa cagttcgaaa
    54121 gatgcgtggt tctcgggtta cggtccgggc gttgtgacct cggtctggat tggctttgat
    54181 gatcaccgtc gtaatctcgg tcatacaacg gcttccggag cgattaaaga tcagatctca
    54241 ggttacgaag gcggtgccaa gagtgcccag cctgcatggg acgcttatat gaaagccgtt
    54301 cttgaaggtg tgccggagca gccgctgacg ccgccaccgg gtattgtgac ggtgaatatc
    54361 gatcgcagca ccgggcagtt agctaatggt ggcaacagcc gcgaagagta tttcatcgaa
    54421 ggtacgcagc cgacacaaca ggcagtgcac gaggtgggaa cgaccattat cgataatggc
    54481 gaggcacagg aattgttctg attaaaaagg cgcttcggcg ccttttcagt ttgctgacaa
    54541 agtgcacttg tttatgccgg atacggcgtg aacgcgttat ccggctaaca aaatcgtgaa
    54601 aactcaataa attgcagaaa ccccataagc ctgataaaca ttgtgcatca ggcaaacttc
    54661 acgcatttac actcgcccct gccctttcaa ccattcgcgc acgaggaaca gcgcactgac
    54721 attgcgcgct tcattgaagt cagggtcttc cagcaaatcc atcatatgcg ccagcggcca
    54781 gcgcacctgt ggtagcggct ctggctcatc gccttccagt gattccgggt agagatcttg
    54841 cgctaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga gcttcttcaa
    54901 aaaagtcaga tcgttcgctc caaatccaac ctcttctttt agctcgcggt tagcggcttc
    54961 gtagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt aggattcagt
    55021 tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc acaatcggca caatcatcac
    55081 tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat tgctgaactc
    55141 cagatccacg ctctcgacgg taaacagtcg ggaacgggct acagtttcaa cattcagaat
    55201 ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc agcggttatt
    55261 gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg ttatttacat ttatgtaact
    55321 taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa ctccaggaat
    55381 ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg atgtgcttaa
    55441 tgatgctcaa gttaaactcc acgcttgccg atagccaacc gcagaatcat gtattgtgtc
    55501 cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga gcaccattgt
    55561 gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga taaaagtgcg
    55621 atccagacgg cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa cgcgtaaact
    55681 cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc aggtattaca
    55741 ggtgcctggc ccaacgggag ccagcgcggc accgatctct ctggcgctga atgccgaaag
    55801 caacaacgtc atgatgctga cacacgctat cacgcgttac ggcatctcta ccgacgatcc
    55861 gaataaatgg cgttactacc tcgattcggt agaagtccac ctgccccctt tctgggaaca
    55921 gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc cgctggttat
    55981 ttcactcaac ggtcatacgc tgcaggagta catgcaggaa actcgcagct atgccttgca
    56041 acctgttccg tcaacgcagg cgtcgattcg cggggaagag agtgagcaaa tcgagctact
    56101 caatattcgc aaagaaacgc atgaagaata tgcgctgagt cgtccgcgcg ggctgcgtga
    56161 agcgttgctg atcgtcgcct ccttcctgat gttctttttc tgcctgatta ccccggatgt
    56221 atttgttccg tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc tgtgggggct
    56281 attcgcgccc ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg gtacaccccg
    56341 tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt cactcggtat
    56401 tatcgacctg gtctatcccg cacactggca gccatacatt gctcaggatc tcggtcaaca
    56461 aaccgatatc gatatctatc tcgaccgcca tgtagtgcgt cagggacgat atctttcgct
    56521 gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga ttatcgctgc
    56581 gggctcgctg ctggtgctgt ttatgctgtt attctggatc ccgctggata tgccgctgaa
    56641 attcactctc tcatggatga aaggcgcgca gaccattgaa gccaccagcg taaaacaact
    56701 ggctgacgct ggcgtgcggg taggcgatac attgcgtatt agcggtacgg gaatgtgtaa
    56761 tattcgaact tccggaacct ggagcgcgaa aaccaattca ccttttttac cgtttgactg
    56821 ctcgcagatc atctggaacg acgcccgctc attgccatta ccagaatctg aactggtcaa
    56881 caaagcgacg gcattgactg aagcagttaa tcgccagctg caccctaaac cggaagatga
    56941 atctcgcgtc agtgcctcat tacgttcagc aattcaaaaa tccggcatgg tattgcttga
    57001 tgattttggc gacattgtac tgaagacagc ggatttatgt tctgccaaag atgactgtgt
    57061 gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg cgctggtaaa
    57121 acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta ttacgcccgg tgagtgcgga
    57181 atcgctggat aacctggtgg caacctccac cgcgccgttt atcacgcatg aaacggcgcg
    57241 agcggcacaa tcactaaaca gtccggcccc cggcggattc ctgattgtca gcgacgaagg
    57301 cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactacc cgccgcaaga
    57361 acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt ttaacgccga
    57421 gggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatattg gccttcatcc
    57481 gatcccggat cgttccggcc tgtggcgcta tctcagcacc acattgctgc tactgacgat
    57541 gctgggtagc gccatttaca atggcgtaca ggcctggcgt cgttaccagc gtcatcgcac
    57601 tcgcatgatg gagattcagg cctattatga aagctgcctg aacccgcaac tgatcacccc
    57661 ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc tgtcgcgcaa
    57721 attgcttcac tctggagatt tccctcatgc atatcaacat tgcctggcag gacgtagata
    57781 ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac tatttctggc
    57841 aaaagctggt gcctgaaaca tggggcgcga aaaacggggt tacgccacag gaagcgatgg
    57901 aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac tgtcttgatt
    57961 actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg ggaccgcgtg
    58021 ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc ggtaagcagc
    58081 gaattttgct caccaatgcg catccgcaca acctggcggt aaaacttgag cataccggtc
    58141 tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg aaagaggatc
    58201 agcggttatg gcatgcggtg gccgaagcta cgggtctgaa agctgaaaga acgctgttta
    58261 ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt tactgcctcg
    58321 gcgtgactaa tcctgattcc gggattgccg agaaacagta tcaacgccat ccgtcactga
    58381 atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag acgccatgaa agaaaaacct
    58441 gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa aacccgcgcg
    58501 ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg cagcaagccg
    58561 agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga cgaacgcacg
    58621 gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc cttgctgtat
    58681 gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg taaacttaat
    58741 gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa aagagcgccg cgacctgtta
    58801 cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat tatgccgcaa
    58861 catgaccaat tacatcgcta tctgtttgaa aactttgccg tgcgcggcga actggtaacc
    58921 gtttcggaaa ccctgcaaca gatccttgag aaccacgatt atccgcagcc cgttaaaaac
    58981 gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgctgaa gtttgatggt
    59041 gatatcaccg tacagctgca gggcgacggt ccgatgaatc tggcggttat taacggtaac
    59101 aataaccagc agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga aaatgccgac
    59161 ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca ttaccccgag cgaaggcgaa
    59221 cgctatcagg gcgtagttgg tctggaaggt gataccctgg cggcctgcct ggaagattac
    59281 tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga cgtagacggc
    59341 aaaccggctg caggcggtat gttgttgcag gtaatgcctg cgcaaaatgc ccagcaggac
    59401 gactttgacc acctggcgac gctaaccgaa accatcaaaa ccgaagaact gctgacctta
    59461 ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt ttacgatccg
    59521 caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc gctgaaaacg
    59581 ctgcctgatg aagaagttga tagcatcctg gcggaagatg gcgaaattga catgcattgt
    59641 gattactgcg gtaaccacta tctgttcaat gcgatggata ttgctgaaat ccgcaacaac
    59701 gcgtctccgg cagatccgca agttcattaa tgagtatgtc cggcagagag ggctctgccg
    59761 gattttacga ctaatacacc tgatgcgctt tgtcactcat tcagattcac attcagcctt
    59821 tcaccatcca ctacaagatc gcgccacgct ttaggcttcg tccccagttt tccatccttc
    59881 gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg
    59941 tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca
    60001 aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag gacgcaggcg
    60061 tctttttcaa tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc
    60121 gcatcaggtt taacagaacc aggggcaatt aacacattat cggctaatac tttttccgat
    60181 accggttgct gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc
    60241 gggtctttca taaacccggc atcgcttttc agcgactcaa gcgcagcacg tccatagcga
    60301 ccgctctgct cgagcatgta gatagttacc tggtctgacg tgtttttgcc gctctgataa
    60361 cgcgccatat ggctgttcac gctaatgcgc atactgtcca gcaccggcga attaagaagc
    60421 accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaacg
    60481 ggattttgcc ctttacgcca gacgatgctg acaaaatacc ccagcgacca caccagtaac
    60541 accagcaccg ccagcacgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc
    60601 agccataacg cccaggcggc gacgaacaca tacagcggag caaccagcaa agcggtttta
    60661 atcaggcaac gcaacggccc tgtccaggga agtgacgctt tttgcggatc gcggacaata
    60721 gccattaaga tcaattgcaa aaaggccagc gtcaacagca acccggcggc ggagatgtgg
    60781 cgagaaatcg cgctcaggcc cgtaaacggc agggtgatga taaacatcag ggttagcaat
    60841 gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat agagtcgatt
    60901 aaacgtgact gtgttcgcgc gaggatcacc gccagcgcgg taaccagacc taacgtgaga
    60961 taaataaacc agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta
    61021 aacaactcac tccacagtaa aagcaccagc cacgtcaagc cattggcgag aaaaatcacc
    61081 agtaatataa gtacgttatg ccatactgac tggtaaaaat agcgatagcg gctactgtca
    61141 ttgcggatgc gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga
    61201 tagcaaccaa aatcccaaag cgccttttca gctctccagg ggttcatgcc atcagtttgc
    61261 cacttcagcc agccgctcat ccccagcgtg gcaataaaca ctagcgccag ccatccccaa
    61321 aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc
    61381 gcaggcacgc cataaacaat ccaactgtga ttttttccgg aaagccaggc gatcagcagg
    61441 tagcaaacca gaccctgaag taatccggta gcaatcatcc cccagcgtgt tgcgggtgaa
    61501 agttcaacgt tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg
    61561 ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat
    61621 actttaccgg ttgaatttgc atcaatttca ttcaggaatg cgattccact cacaatattc
    61681 ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt
    61741 ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt
    61801 tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact
    61861 attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc
    61921 cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct
    61981 acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt
    62041 taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag
    62101 ataagtatat cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca
    62161 aaggtaagaa cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg
    62221 tgaccaggca gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc
    62281 cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca
    62341 aaaacatgtt tattcgcccg agcgatgaag aactggcagg tttcaaacca gactttatcg
    62401 ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctc aactccgaaa
    62461 acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg
    62521 gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg
    62581 cttctatgca ctgctccgcc aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc
    62641 tttccggcac cggtaaaacc accctttcca ccgacccgaa acgtcgcctg attggcgatg
    62701 acgaacacgg ctgggacgat gacggcgtgt ttaacttcga aggcggctgc tacgcaaaaa
    62761 ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgtgatgcgt
    62821 tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa
    62881 ccgagaacac ccgcgtttct tatccgatct atcacatcga taacattgtt aagccggttt
    62941 ccaaagcggg ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc
    63001 cgccggtttc tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg
    63061 ccaaactggc cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct
    63121 tcggcgcggc attcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta
    63181 tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac
    63241 gtatctcgat taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata
    63301 atgcagaaac cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg
    63361 tagacacgaa gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa
    63421 aagccgaaac cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg
    63481 cgggtgccgc gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc
    63541 tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct
    63601 tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc
    63661 ccgccccgct cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc
    63721 acaattgcca gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac
    63781 ggctggaaca ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg
    63841 aaccaggcgc gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg
    63901 gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcact
    63961 tcaatgctgc cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca
    64021 atcacctcac cgagtactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg
    64081 cgcaggtagt cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc
    64141 gattctgcca gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc
    64201 agcggcgtgc gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt
    64261 tgcttaacac cagccgccat atggttaaag gcacgggtaa cggaacgcac ctcggacgcg
    64321 ccatactcac gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg
    64381 agatcgacca acggtctgtt ctggatacga ataaacagcc acgccccgcc tatcgccaat
    64441 agcataatcg ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc
    64501 agcggcacgc gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa
    64561 cttttgttga cctcaacgcg cacttccgtc gggccgccca gttgctgcgc catctgatgg
    64621 cttaagaatt catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag
    64681 tagagagaga tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac
    64741 tgcgtgccgt cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg
    64801 actttattaa actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc
    64861 gtcaccaggc tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt
    64921 cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca
    64981 gaccccagac ggtctgaatg taacgcggat gcgctggatc ttcttccacc atgcggcgca
    65041 gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa
    65101 ggttcatcag cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta
    65161 ccgcaaactc accgctggtg agcggcatcg gctcgtcttc gcggaacatt tcgcgcgtac
    65221 cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg
    65281 gcagttcgtt cgcctgacga cgcagcaccg cacggatacg ggccagcagt tcacgcgggt
    65341 taaacggttt tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt
    65401 cttccccttt cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc
    65461 ggcaaatcga caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag
    65521 attcacgagt cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc
    65581 cttgttcggt gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca
    65641 gaatcttgta gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg
    65701 tgtattctta aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa
    65761 attgttacaa agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat
    65821 tgattctgtt gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga
    65881 atcaacgaga tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag
    65941 cttaattatc tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca
    66001 agtctgggcg accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa
    66061 atcgaccgtc gcgtgcgcta tctcactaaa tgcctggaaa atctcaaaat cgtcgattac
    66121 tcccctcagc aggaaggcaa agtctttttt ggcgcgtggg tggagattga aaacgacgat
    66181 ggcgtgactc accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac
    66241 atctctatcg attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg
    66301 gtggtgaata cccctgccgg ggaagcgagc tggtatgtta atgctatcga gtacgtgaaa
    66361 ccgtaaggaa gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat
    66421 aactatcccc tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc
    66481 tgccgcatta ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc
    66541 ctgcttgacg aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc
    66601 ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg
    66661 gaagagagac gtcaggcgat cctcaagtcc atttccgagc aaggcaaact caccgatgat
    66721 ctggcgaagg ccatcaacgc caccctaagc aaaaccgaac tcgaagacct ctacctgccc
    66781 tacaaaccta aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg
    66841 gctgacctgc tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt
    66901 gatgccgata aaggcgtggc agataccaaa gccgcgctgg atggcgcgcg ctatatcctg
    66961 atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag
    67021 aacgcgcatt tggtttctac ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc
    67081 gactatttcg atcatcacga accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg
    67141 ttccgtgggc gtaacgaagg cgtactccag ctttcgctga atgccgatcc gcagttcgat
    67201 gagccgccca aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctacgcctg
    67261 aacaatgccc cggcggatag ctggcgcaaa ggcgtagtga gctggacctg gcgcatcaag
    67321 gtgctgatgc atctggaaac cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa
    67381 gcaatcaacg tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg
    67441 cgtgcaacga tgggcctcga tccgggtctg cgtactgggg taaaagtggc ggtggtcgat
    67501 gccactggca aactggtagc gaccgatacc atttacccgc acaccggaca agccgcaaaa
    67561 gcagcgatga ccgttgctgc cttgtgtgaa aaacataacg ttgaactggt ggcgatcggc
    67621 aacggtacag cttcccgcga aactgaacgt ttctatctcg acgtgcagaa gcagttcccg
    67681 aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc
    67741 gagctggcag cgcaggagtt cccggatctc gacgtttcgc tgcgtggcgc ggtgtctatc
    67801 gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc
    67861 gtaggtcagt atcagcatga cgtcagccag acgcaactgg cccgcaaact ggacgcagta
    67921 gtagaagact gcgtgaacgc cgttggcgtc gatctcaaca ctgcttctgt tccgctgtta
    67981 acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag
    68041 aacggccagt tccagaaccg tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc
    68101 ttcgagcagt gcgcgggctt cttgcgcatt aaccacggtg ataacccgct ggatgcttct
    68161 accgtccacc cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca
    68221 ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact
    68281 gatgaaaaat tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt
    68341 cgcgatccgc gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac
    68401 gacctgcaac cgggtatgat cctcgaaggt gcggtgacca acgtcaccaa ctttggcgcg
    68461 tttgtcgata tcggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag
    68521 tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg
    68581 gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc gtctggacga gcagcctggc
    68641 gaaaccaacg cccgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc
    68701 aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg
    68761 gcggcaatgg gcaaaaaacg ttaaacgccc ggactggccc ctaaacctgc gcgataaggc
    68821 gttcacgccg catctggcaa cgaacgcctt gcctgacata aaagtgccgg agaatatctc
    68881 cggccttttt attccacaac caaactcata atatattccg gcaatattta tcacttcatt
    68941 aacaactgaa accttaatta aacattagcc agtccgggta attcactatt cgaattatat
    69001 tttcgctgcg atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa
    69061 cattcgcaca cattttaagt attgctgata gaaaccattc tcattatcat tgtgttgttg
    69121 attatttaat ctctcctttg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc
    69181 atgaggtagt tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata
    69241 ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc
    69301 tttctcttgg catgttacct ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg
    69361 accccattca tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct
    69421 tattagaagt ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc
    69481 ataggcttaa ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc
    69541 gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc
    69601 tccaccaccg atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc
    69661 atctcatcgc agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac
    69721 gccgacctgc tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg
    69781 ctacaactgc tggaactcgg cattccctgc attgtggcac tgaacatgct cgacattgcc
    69841 gagaagcaaa atattcgtat tgaaattgat gctctgtcgg cgcgtctggg ctgtccggtg
    69901 atcccgctgg tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc
    69961 tataaagcta acgagaatgt ggaactggta cattacgcac agccgctact caacgaagca
    70021 gattcactgg caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc
    70081 ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat
    70141 cttgatgccg ccctcgcccg tctgcgtaat gagatggacg atccggcgct gcacattgcc
    70201 gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca
    70261 gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg
    70321 ccgattttcc tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg
    70381 ttacagccgc tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt
    70441 ggctacacgc tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc
    70501 ggcattaaca ccgtgctgcc actggtgccg cagattggca tgatgtacct gttcctctcc
    70561 ttccttgagg actccgggta tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag
    70621 gcgctgggct tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta
    70681 ccgtcggtaa tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg
    70741 atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc
    70801 ttcttcgggc agaacggtgc gctggcggtc ttctcgctgt atatgctggg tattgtgatg
    70861 gcggtgctga ctggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt
    70921 gtcatggagt tgccggtcta tcatgtacca cacgttaaaa gcctgattat ccagacctgg
    70981 cagcgtctga aaggcttcgt tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc
    71041 ctgagcgctt tcaacagctt ctcgctgagc gggaaaatcg tcgataacat caacgactcg
    71101 gcgctggcgt ccgtcagccg ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa
    71161 gataactggc aggcaacggt tggcctgttt acaggtgcca tggcgaaaga agtggtagtg
    71221 ggtacgctca acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggca
    71281 gaatttaacc tcggtgaaga gctgttcagt gcgatagatg aaacctggca gagcctgaaa
    71341 gacaccttca gccttagcgt actgatgaac cccattgaag ccagcaaagg cgacggcgaa
    71401 atgggtaccg gggcgatggg cgtgatggat cagaaattcg gtagcgcagc agcagcttac
    71461 agctacctga ttttcgtcct gctgtatgta ccatgtatct cggtgatggg ggctatcgcc
    71521 cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac
    71581 tcactggcaa cattgttcta tcaggtcgcc agctacagtc agcatccaac ttacagcctg
    71641 gtgtgcattc tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg
    71701 cgtagccggg tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca
    71761 gccagcacca ccggtgattg ccattaatgg cttcacttat tcaggtgcgc gatttgctgg
    71821 cgttacgggg ccgtatggaa gcggcccaga taagccagac attgaacact ccacagccaa
    71881 tgattaacgc catgctgcaa caactggaaa gtatgggcaa agccgtgcgg attcaggaag
    71941 aacctgacgg ctgcctctct ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc
    72001 gcgagtggtg ggcgctgcgt taaccttact ccatcgcctg ttttggaaag cccggtatgc
    72061 gtctgcatcc gggctttttt gcgtgcggct ttccataaaa atgcaactct tgcagcacgg
    72121 cgtaagttcc tttgaaagca tctcgcaggg atgaaaactc gctaatacac aggtgtggag
    72181 tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga acgccatgag caaaaagcag
    72241 agttccaccc cacacgatgc gctgttcaaa ctctttttac gccaaccgga cacggctcgt
    72301 gattttcttg cgtttcattt accggcaccc attcacgcgc tttgtgatat gaaaaccctc
    72361 aagctggagt cgagcagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg
    72421 tggtcggtga aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc
    72481 acctcaaaca aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat
    72541 caccttgatg ctggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt
    72601 attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg
    72661 gcaaggcagc tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat
    72721 gaaatcatgc agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa
    72781 cgcgatctga tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat
    72841 gacagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgacgc ggtacgtttt
    72901 aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgaca
    72961 attgcggaaa gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata
    73021 atgcttgaat ccggagtccc tcttacagac atcatgcgct ttaccggggt gtcagaagaa
    73081 gagttggctg cggcgagcca gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc
    73141 tctgcttcaa cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat
    73201 gggccgcttt ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca
    73261 ccactttgcg cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatggaca
    73321 cgttttgcag cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca
    73381 cgtcaacctc cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc
    73441 gcgccgtttc agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat
    73501 catcactgag ttgctgctga aatcccgcca gcacgtccgg ttttatcccc ggccactcgt
    73561 cacgagcact aaaacaaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat
    73621 gggttaaaac aatttggctt gccaccagcc cgcccagact ccagcctaac caaatggctt
    73681 tatcaggtgc ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc
    73741 cccggctacg cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt
    73801 cctcgtcaat gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat
    73861 gaacattccc ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt
    73921 tgacttacaa ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc
    73981 cactggcgtt aggtcattgg gggatttgtt cggtctgttc acgcgccgcc cgtacagata
    74041 aaacgttatg cccacaatgt ggattacccg ccacacactc ccatcttccc tgcggtcgtt
    74101 gcctgcaaaa accgccgccc tggcaaagac tggtcacggt tgcggactat gcgccgccgt
    74161 taagtccgct aatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt
    74221 cacgtctgtt actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg
    74281 atcgcatcat cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga
    74341 gcgatttgct gtgtcagccg ttatcacgct ggttgcactg ccgatgggat agcgaagccg
    74401 tcacacgaac acgggccact gcgacccagc attttctcag tgcccggcta cgcaagcgca
    74461 acctgaaaaa tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg
    74521 atgatgtcgt taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg
    74581 gtgcggcgac tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc
    74641 gtattataac caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat
    74701 gctgcacaag cgcactttgc caaactgctg gcaaaccagg aagaagggac acaaatccgc
    74761 gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg
    74821 gatgccgtgg aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat
    74881 gagttaagcg ctccttacct ggaagatgca gagatcgatt ttgttaccga ccagttgggt
    74941 tctcagctaa cactgaaagc cccgaacgcc aaaatgcgta aagtggcaga cgatgcaccg
    75001 ctgatggagc gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac
    75061 ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc
    75121 ggcggctgta acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag
    75181 ctgctgaacg aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc
    75241 gaacactcct actactaagt tatcccgtct tttgtagatt gcccgatgcg acgctaaagc
    75301 gtcatttcgg gcctacgttg aacatgtgcc agacgttggc gcagccttgc tttggcaaat
    75361 cctcggttcg tttccccgcg ataatatgac caacctctca taatttaaat ttaccccgct
    75421 ctggtgattc tcaaacgcca gatgttaccc gtatcattca catgggtacc aaacatactc
    75481 ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc gcaatatctc
    75541 tgttcccgat tgggataatt agagtttgtc gtcagaaaat tgacgttacc cataacaaat
    75601 gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc ttgttgttgc
    75661 tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg gcgcttgctg
    75721 ttggattaat gcaaggaatg ccgctggata aagttattgg ctccatcaaa gccggtgtcg
    75781 gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg ggcaaaatgc
    75841 tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa tttggtaaaa
    75901 aacacatcca gtgggcggtg gtactgaccg gttttaccgt tggttttgcc ctgttctatg
    75961 aagtgggctt tgtgctgatg ctgccgctgg tgtttaccat cgcggcttct gcgaatattc
    76021 cactgctgta tgttggtgta ccaatggcag ctgcactgtc tgtgacccac ggcttcctgc
    76081 caccgcatcc gggtccgact gcgattgcca ccattttcaa tgccgatatg ggtaaaaccc
    76141 tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg gtttacgctc
    76201 gcgtgttgaa aggtatcgat aagccaattc cggaaggtct ctacagcgcg aaaaccttca
    76261 gtgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg ccggtagtac
    76321 tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct ttcctgccgg
    76381 tagcggagtt cctcggtgac ccggtaatgg ctacgctgat tgccgtgctg attgcgatgt
    76441 tcacctttgg tctaaaccgt ggtcgttcaa tggatcagat taacgacacg ctggtttctt
    76501 ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg ttcaagcagg
    76561 tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa accaacattt
    76621 ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg ggttccgcaa
    76681 ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg acgggtgtta
    76741 gcccggagct gatggttatt gcggttggtt ccggtagtgt gattttctct catgtgaacg
    76801 atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag accatcaaat
    76861 cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt ctgctgctga
    76921 atatggtgat ttgataacac tgactgccgg atgcggcgtg accgccttat ccggcctacg
    76981 attcgggatg aattagtagg ccggataaga cgcgtcaagc atcgcatccg gcatcaaccg
    77041 cactctactt cttcttcgct gcagctctgc ggcgtctgtc caaatccttc agcaacttgt
    77101 tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt cgccagtttt
    77161 tatactggta actggtgcca ggaatattca ccggttcggc catatccagc cagtcttccg
    77221 gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa cctcgattaa
    77281 gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc aggcaaccat
    77341 atttatgcag agcatccagc agtccttgtt tcgccagttc acgatcctga tacagaccgc
    77401 gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga tccccgctct
    77461 cccagtagcc gcgtagcgta ggcaggtcat gtgtcgccgc aaccgccatc gactgctccg
    77521 gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag agcactttgt
    77581 aggagtacac accgctgctg cgcagcttac cgacaatctc taccggtacg gtaccgagat
    77641 cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc gagagcagat
    77701 catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac ggtatccacc
    77761 acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt tgcatattgg
    77821 cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc gggtccattg
    77881 gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg ccaaccgacg
    77941 ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc gctacgccaa
    78001 ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc tcccagcagg
    78061 cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg tcatcacgat
    78121 gttcttcgca gaactgacgc acttctggtg aatccacgtt ctgatacatc tctggccatg
    78181 caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc gcatcaaagg
    78241 ctgcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac gcggtcatct
    78301 gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc atttttaggg
    78361 cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc gtctgttgcg
    78421 tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct tcaacggcgt
    78481 taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat gggctggcac
    78541 tctccggatt tgccggatag agcgcatgaa tcgggttcag gccgatgaac gacccgccac
    78601 gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata ccccagtttt
    78661 tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt ttattcagca
    78721 acgcctgcgg ttcgtaacag cgtttcgggg cgacaatcac ccggcaatgc gcgcgctggt
    78781 cgtcctgggt gagtgtcagc gtgtgataac cttccggcag cttcgtcggt agattgaacg
    78841 ctttgccccc agttacatgg cctttgtact gcgttccttc ttcggtggtc agcagccagc
    78901 tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta taaaccatga
    78961 cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc gcgtcaagca
    79021 aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg atgtaattgg
    79081 ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc gagcttcctt
    79141 agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc gagctaaaca
    79201 taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg tcgcggtaca
    79261 gcacatccac ctgcttttgt gcctctacat aagctgcgaa atccgccatc accagatacg
    79321 gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc ttatcgccgt
    79381 cgctgtattt gccgctttcc agctctttca atactgcgtc cagcacctta tctttcttcc
    79441 gccatttcac cgggtcgtag cctttggcca gaattgcctt cacttgttcc acggtatgac
    79501 caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc gccccatcca
    79561 gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta ccggaagctt
    79621 ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt ttttccgccg
    79681 ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc agcggatcgt
    79741 tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc aggtagtagc
    79801 ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc tgcgggtttt
    79861 cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg cgtttgtact
    79921 catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca ataccggtac
    79981 gaactttcac aaactccgcc agacggactt tattcgcctg cttgatctcg cgatattgct
    80041 gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg agatcgttag
    80101 cccactcttt ttgcagtgat ttatccaaca gagccgccag tgccgggttg cactgtttga
    80161 tccagcgacg tggggtaata ccgttggtga cgttatggaa tttgttcggc catagctggt
    80221 gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca acaccgttca
    80281 ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttgtcg tgcaccaccg
    80341 ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctctaccagc gttttaaagc
    80401 gagtattaat ttcgttaata atctgcatgt ggcgcggcag taagcctttc accagtttca
    80461 catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg aaagttttgc
    80521 tggtaatggc ccaggcgtca tcccagctca tctggtgctc atcgatcagc acgcgcagca
    80581 gttctggaat cgcgatagtt gggtgggtat cgttcagctg aataacttcg taatccgcca
    80641 gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct accgaacagg
    80701 cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg ttgtcgtttg
    80761 gatagagaac tttggtcagt ttttccgcat tgatgccctg ctgttcggca cgcaagaaat
    80821 caccgtcgtt aaatttagtc agatcaaacg gatgcgcgtg cgtcgcctgc cacagacgca
    80881 gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct tgaccggtaa
    80941 tggtaaactc cggctcccag cgtccgtctt tcgtcacttt accgccaatc cctacctgca
    81001 catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag tcatccggcg
    81061 cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga tagttcagac
    81121 cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac gccgccagac
    81181 gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg tccgtcagat
    81241 tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga ttcaacaggt
    81301 tgttgcccgt caggcgacca atcaaaaact ccattgagat gtagttaaca tgtcgctgat
    81361 tcgccaccgg cttggcgaat ggctgagcac gcagcatttc ggccagtgct tcactcactg
    81421 ctagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa cgctgccact
    81481 gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac ataggagttc
    81541 cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg atgttgacct
    81601 tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag gaaatgtgat
    81661 ctcaaccact taaagctagt gcaaaccaca ggattagcat caaatcaatg caatacagcg
    81721 cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa agcggaaggt
    81781 aactctataa attaagtaaa ggagtgaaac agtttcataa gtaaaatatc cagtgtgctc
    81841 catctcattc ttaatagatt tattaagatc atctttttag atggcacttt catcaggaat
    81901 gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag tattcacgct
    81961 ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac acagtgcaaa
    82021 ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc gaccttataa
    82081 ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt gaagtgatta
    82141 actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac cgtggttcgt
    82201 gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct gatcacgagt
    82261 cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa aaacgatatc
    82321 ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag ctatctcatt
    82381 gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat ggcgcaaaaa
    82441 cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct ggcggaatgg
    82501 catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc agtgatccac
    82561 gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt ggtgttgtca
    82621 cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgatcaact gctggaaatt
    82681 ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga ttgccgtctg
    82741 tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc cggttgggcg
    82801 acggcactac agctaatcgc cctctccgcc cggcagaata cccactcagc ccataagtcg
    82861 gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt cgatgaggtt
    82921 ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat tttgcgctca
    82981 atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat gcgcctcgaa
    83041 gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga gtggttctgc
    83101 tatcacccgc tgtttggtaa cttcctgcgc cagcgctgcc agtgggaact ggcggcggag
    83161 ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt tcccagcgaa
    83221 gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat tctgcttaat
    83281 cacgcctgga gtctgttcaa ccatagcgaa ctgtcgctgc tggaagagtc gcttaaggcc
    83341 ctgccgtggg acagcttgct ggaaaatccg cagttggtgt tattgcaggc gtggctgatg
    83401 caaagccaac atcgctacgg cgaagttaac accctgctag cccgtgctga acatgaaatc
    83461 aaggacatca gagaagacac catgcacgca gaatttaacg ctctgcgcgc ccaggtggcg
    83521 attaacgatg gtaatccgga tgaagcggaa cggctggcaa aactggcact ggaagagctg
    83581 ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga agtgctgcac
    83641 tgcaaaggcg aattgacccg ctcactggcg ctaatgcagc aaaccgaaca gatggcacgc
    83701 cagcacgatg tctggcacta cgctttgtgg agtttaatcc agcaaagtga aattctgttt
    83761 gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcattcca gctgatcaac
    83821 gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcattcg tgcgcagctg
    83881 ttatgggcct gggcgcggct ggatgaagcc gaagcgtcgg cgcgtagcgg gattgaagtc
    83941 ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat tcaatgctcg
    84001 ctggcccgtg gtgatttaga taacgcccgt agccagctga accgtctgga aaacctgctg
    84061 gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg ggtgatttac
    84121 tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac ggctaaacca
    84181 gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg tgcacaaatc
    84241 ttgctgggcg agtttgaacc ggcagaaatt gttctcgaag aactcaatga aaatgcccgg
    84301 agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca actgtactgg
    84361 caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa actggcgaat
    84421 cgcaccggat ttatcagcca ttttgtcatc gaaggcgaag cgatggcgca acaactgcgt
    84481 cagctgattc agcttaatac gctgccggaa ctggaacagc atcgcgcgca gcgtattctg
    84541 cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa tttcgttgaa
    84601 cgtctgctaa atcatcctga agtacctgaa ctgatccgca ccagcccgct gacgcaacgt
    84661 gaatggcagg tactggggct gatctactct ggttacagca atgagcaaat tgccggagaa
    84721 ctggaagtcg cggcaaccac catcaaaacg catatccgca atctgtatca gaaactcggc
    84781 gtggcccatc gccaggatgc ggtacaacac gcccagcaat tgctgaagat gatggggtac
    84841 ggcgtgtaag tttagccgga taacgcgcca gatccggctt acatctctgc atcattcaat
    84901 gctcacccgc gttacgccat ctgtttctat caaactaaac cgcaccggca agaaacgctc
    84961 caccaccgcg atattggtca gcagatggca tgagggatgg gcgaccgtaa attcccccgc
    85021 gcccgccagc gccatcggta gcaccagttg gtcagcaaga tattccccca ccgccgccgt
    85081 gcttgccagg tagcgtttca cctctttcac caactgtgcc gcaaccacct cggcactgac
    85141 gc
//
LOCUS       DAZFZC010000007        81211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFZC010000007 DAZFZC010000000
VERSION     DAZFZC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.007"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            182..808
                     /gene="ydhY"
                     /locus_tag="ACHKXP_000576"
     CDS             182..808
                     /gene="ydhY"
                     /locus_tag="ACHKXP_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO7645647.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..2931
                     /gene="ydhV"
                     /locus_tag="ACHKXP_000577"
     CDS             829..2931
                     /gene="ydhV"
                     /locus_tag="ACHKXP_000577"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO7645648.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRVTTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAVDLAAHNLLPV"
     gene            2944..3582
                     /gene="ydhW"
                     /locus_tag="ACHKXP_000578"
     CDS             2944..3582
                     /gene="ydhW"
                     /locus_tag="ACHKXP_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO7645649.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3646..4314
                     /gene="ydhX"
                     /locus_tag="ACHKXP_000579"
     CDS             3646..4314
                     /gene="ydhX"
                     /locus_tag="ACHKXP_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7645650.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4311..5096
                     /gene="phsC"
                     /locus_tag="ACHKXP_000580"
     CDS             4311..5096
                     /gene="phsC"
                     /locus_tag="ACHKXP_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO7645651.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPLPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5100..5912
                     /gene="ydhT"
                     /locus_tag="ACHKXP_000581"
     CDS             5100..5912
                     /gene="ydhT"
                     /locus_tag="ACHKXP_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO7645652.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALVYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5924..7528)
                     /gene="ydhS"
                     /locus_tag="ACHKXP_000582"
     CDS             complement(5924..7528)
                     /gene="ydhS"
                     /locus_tag="ACHKXP_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO7645653.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFHGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7654..7959)
                     /gene="ydhR"
                     /locus_tag="ACHKXP_000583"
     CDS             complement(7654..7959)
                     /gene="ydhR"
                     /locus_tag="ACHKXP_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO7645654.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8067..8143)
                     /locus_tag="ACHKXP_000584"
     tRNA            complement(8067..8143)
                     /locus_tag="ACHKXP_000584"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8107..8109),aa:Val,seq:gac)
     gene            complement(8148..8224)
                     /locus_tag="ACHKXP_000585"
     tRNA            complement(8148..8224)
                     /locus_tag="ACHKXP_000585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8188..8190),aa:Val,seq:gac)
     gene            8532..9788
                     /gene="ydhQ"
                     /locus_tag="ACHKXP_000586"
     CDS             8532..9788
                     /gene="ydhQ"
                     /locus_tag="ACHKXP_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7645655.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGTGWLKVATGGIVKCTQYGNNGTLSVSDGAIATDVVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="ACHKXP_000587"
     CDS             complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="ACHKXP_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO7645656.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11417..12058
                     /gene="ribE"
                     /locus_tag="ACHKXP_000588"
     CDS             11417..12058
                     /gene="ribE"
                     /locus_tag="ACHKXP_000588"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO7645657.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="ACHKXP_000589"
     CDS             complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="ACHKXP_000589"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO7645658.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13537..14748)
                     /gene="punC"
                     /locus_tag="ACHKXP_000590"
     CDS             complement(13537..14748)
                     /gene="punC"
                     /locus_tag="ACHKXP_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO7645659.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGHAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14861..15793
                     /gene="punR"
                     /locus_tag="ACHKXP_000591"
     CDS             14861..15793
                     /gene="punR"
                     /locus_tag="ACHKXP_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO7645660.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15790..16815)
                     /gene="purR"
                     /locus_tag="ACHKXP_000592"
     CDS             complement(15790..16815)
                     /gene="purR"
                     /locus_tag="ACHKXP_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO7645661.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17114..17203
                     /gene="cydH"
                     /locus_tag="ACHKXP_000593"
                     /gene_synonym="ynhF"
     CDS             17114..17203
                     /gene="cydH"
                     /locus_tag="ACHKXP_000593"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO7645662.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17369..18538
                     /gene="ydhP"
                     /locus_tag="ACHKXP_000594"
     CDS             17369..18538
                     /gene="ydhP"
                     /locus_tag="ACHKXP_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7645663.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18773..19354)
                     /gene="sodB"
                     /locus_tag="ACHKXP_000595"
     CDS             complement(18773..19354)
                     /gene="sodB"
                     /locus_tag="ACHKXP_000595"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO7645664.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19482..20297)
                     /gene="mepH"
                     /locus_tag="ACHKXP_000596"
     CDS             complement(19482..20297)
                     /gene="mepH"
                     /locus_tag="ACHKXP_000596"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO7645665.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20631..20978
                     /gene="grxD"
                     /locus_tag="ACHKXP_000597"
     CDS             20631..20978
                     /gene="grxD"
                     /locus_tag="ACHKXP_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO7645666.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21029..25645)
                     /gene="lhr"
                     /locus_tag="ACHKXP_000598"
     CDS             complement(21029..25645)
                     /gene="lhr"
                     /locus_tag="ACHKXP_000598"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO7645667.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAAKTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25738..26385)
                     /gene="rnt"
                     /locus_tag="ACHKXP_000599"
     CDS             complement(25738..26385)
                     /gene="rnt"
                     /locus_tag="ACHKXP_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO7645668.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26488..26895)
                     /gene="gloA"
                     /locus_tag="ACHKXP_000600"
     CDS             complement(26488..26895)
                     /gene="gloA"
                     /locus_tag="ACHKXP_000600"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO7645669.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26976..28073)
                     /gene="nemA"
                     /locus_tag="ACHKXP_000601"
     CDS             complement(26976..28073)
                     /gene="nemA"
                     /locus_tag="ACHKXP_000601"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO7645670.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28110..28709)
                     /gene="nemR"
                     /locus_tag="ACHKXP_000602"
     CDS             complement(28110..28709)
                     /gene="nemR"
                     /locus_tag="ACHKXP_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO7645671.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28812..29051
                     /gene="ydhL"
                     /locus_tag="ACHKXP_000603"
     CDS             28812..29051
                     /gene="ydhL"
                     /locus_tag="ACHKXP_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO7645672.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29100..29996
                     /gene="ydhF"
                     /locus_tag="ACHKXP_000604"
     CDS             29100..29996
                     /gene="ydhF"
                     /locus_tag="ACHKXP_000604"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO7645673.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30077..30598
                     /gene="sodC"
                     /locus_tag="ACHKXP_000605"
     CDS             30077..30598
                     /gene="sodC"
                     /locus_tag="ACHKXP_000605"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7645674.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHAGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30599..32611)
                     /gene="ydhK"
                     /locus_tag="ACHKXP_000606"
     CDS             complement(30599..32611)
                     /gene="ydhK"
                     /locus_tag="ACHKXP_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7645675.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALDSSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCRLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAASELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32611..33468)
                     /gene="ydhJ"
                     /locus_tag="ACHKXP_000607"
     CDS             complement(32611..33468)
                     /gene="ydhJ"
                     /locus_tag="ACHKXP_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7645676.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33471..33707)
                     /gene="ydhI"
                     /locus_tag="ACHKXP_000608"
     CDS             complement(33471..33707)
                     /gene="ydhI"
                     /locus_tag="ACHKXP_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO7645677.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33908..34342
                     /gene="slyA"
                     /locus_tag="ACHKXP_000609"
     CDS             33908..34342
                     /gene="slyA"
                     /locus_tag="ACHKXP_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO7645678.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34389..34856)
                     /gene="slyB"
                     /locus_tag="ACHKXP_000610"
     CDS             complement(34389..34856)
                     /gene="slyB"
                     /locus_tag="ACHKXP_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO7645679.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35130..36239
                     /gene="anmK"
                     /locus_tag="ACHKXP_000611"
     CDS             35130..36239
                     /gene="anmK"
                     /locus_tag="ACHKXP_000611"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO7645680.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36337..36666
                     /gene="mliC"
                     /locus_tag="ACHKXP_000612"
     CDS             36337..36666
                     /gene="mliC"
                     /locus_tag="ACHKXP_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO7645681.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36725..37381
                     /gene="pdxH"
                     /locus_tag="ACHKXP_000613"
     CDS             36725..37381
                     /gene="pdxH"
                     /locus_tag="ACHKXP_000613"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO7645682.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37510..38784
                     /gene="tyrS"
                     /locus_tag="ACHKXP_000614"
     CDS             37510..38784
                     /gene="tyrS"
                     /locus_tag="ACHKXP_000614"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO7645683.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38843..39706
                     /gene="pdxY"
                     /locus_tag="ACHKXP_000615"
     CDS             38843..39706
                     /gene="pdxY"
                     /locus_tag="ACHKXP_000615"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO7645684.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39750..40355)
                     /gene="gstA"
                     /locus_tag="ACHKXP_000616"
     CDS             complement(39750..40355)
                     /gene="gstA"
                     /locus_tag="ACHKXP_000616"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO7645685.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40461..41963)
                     /gene="dtpA"
                     /locus_tag="ACHKXP_000617"
     CDS             complement(40461..41963)
                     /gene="dtpA"
                     /locus_tag="ACHKXP_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO7645686.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42574..43209)
                     /gene="nth"
                     /locus_tag="ACHKXP_000618"
     CDS             complement(42574..43209)
                     /gene="nth"
                     /locus_tag="ACHKXP_000618"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO7645687.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43209..43904)
                     /gene="rsxE"
                     /locus_tag="ACHKXP_000619"
     CDS             complement(43209..43904)
                     /gene="rsxE"
                     /locus_tag="ACHKXP_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO7645688.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(43908..44528)
                     /gene="rsxG"
                     /locus_tag="ACHKXP_000620"
     CDS             complement(43908..44528)
                     /gene="rsxG"
                     /locus_tag="ACHKXP_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO7645689.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLITAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44532..45590)
                     /gene="rsxD"
                     /locus_tag="ACHKXP_000621"
     CDS             complement(44532..45590)
                     /gene="rsxD"
                     /locus_tag="ACHKXP_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO7645690.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCAMLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45591..47813)
                     /gene="rsxC"
                     /locus_tag="ACHKXP_000622"
     CDS             complement(45591..47813)
                     /gene="rsxC"
                     /locus_tag="ACHKXP_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO7645691.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKREQQPANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAVPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(47806..48384)
                     /gene="rsxB"
                     /locus_tag="ACHKXP_000623"
     CDS             complement(47806..48384)
                     /gene="rsxB"
                     /locus_tag="ACHKXP_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO7645692.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48384..48965)
                     /gene="rsxA"
                     /locus_tag="ACHKXP_000624"
     CDS             complement(48384..48965)
                     /gene="rsxA"
                     /locus_tag="ACHKXP_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO7645693.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(49042..49482)
                     /gene="ydgK"
                     /locus_tag="ACHKXP_000625"
     CDS             complement(49042..49482)
                     /gene="ydgK"
                     /locus_tag="ACHKXP_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO7645694.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIALWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49568..49783)
                     /gene="ydgT"
                     /locus_tag="ACHKXP_000626"
     CDS             complement(49568..49783)
                     /gene="ydgT"
                     /locus_tag="ACHKXP_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO7645695.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(50056..50181)
                     /gene="blr"
                     /locus_tag="ACHKXP_000627"
     CDS             complement(50056..50181)
                     /gene="blr"
                     /locus_tag="ACHKXP_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO7645696.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            50424..51464
                     /gene="ydgJ"
                     /locus_tag="ACHKXP_000628"
     CDS             50424..51464
                     /gene="ydgJ"
                     /locus_tag="ACHKXP_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7645697.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51498..52499)
                     /gene="add"
                     /locus_tag="ACHKXP_000629"
     CDS             complement(51498..52499)
                     /gene="add"
                     /locus_tag="ACHKXP_000629"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO7645698.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52603..53775)
                     /gene="malY"
                     /locus_tag="ACHKXP_000630"
     CDS             complement(52603..53775)
                     /gene="malY"
                     /locus_tag="ACHKXP_000630"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO7645699.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53785..55377)
                     /gene="malX"
                     /locus_tag="ACHKXP_000631"
     CDS             complement(53785..55377)
                     /gene="malX"
                     /locus_tag="ACHKXP_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO7645700.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLLPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDLGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55552..56580
                     /gene="malI"
                     /locus_tag="ACHKXP_000632"
     CDS             55552..56580
                     /gene="malI"
                     /locus_tag="ACHKXP_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO7645701.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKGIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSN
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56691..57458
                     /gene="hdhA"
                     /locus_tag="ACHKXP_000633"
     CDS             56691..57458
                     /gene="hdhA"
                     /locus_tag="ACHKXP_000633"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO7645702.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57679..58269
                     /gene="uidR"
                     /locus_tag="ACHKXP_000634"
     CDS             57679..58269
                     /gene="uidR"
                     /locus_tag="ACHKXP_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO7645703.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58660..60471
                     /gene="uidA"
                     /locus_tag="ACHKXP_000635"
     CDS             58660..60471
                     /gene="uidA"
                     /locus_tag="ACHKXP_000635"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO7645704.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60468..61841
                     /gene="uidB"
                     /locus_tag="ACHKXP_000636"
     CDS             60468..61841
                     /gene="uidB"
                     /locus_tag="ACHKXP_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO7645705.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLN
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61880..63145
                     /gene="uidC"
                     /locus_tag="ACHKXP_000637"
     CDS             61880..63145
                     /gene="uidC"
                     /locus_tag="ACHKXP_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO7645706.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DNPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGGSVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(63371..64879)
                     /gene="ydgA"
                     /locus_tag="ACHKXP_000638"
     CDS             complement(63371..64879)
                     /gene="ydgA"
                     /locus_tag="ACHKXP_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7645707.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(64980..66155)
                     /gene="manA"
                     /locus_tag="ACHKXP_000639"
     CDS             complement(64980..66155)
                     /gene="manA"
                     /locus_tag="ACHKXP_000639"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO7645708.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66354..68000
                     /gene="fumB"
                     /locus_tag="ACHKXP_000640"
     CDS             66354..68000
                     /gene="fumB"
                     /locus_tag="ACHKXP_000640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7645709.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            68143..69546
                     /gene="fumC"
                     /locus_tag="ACHKXP_000641"
     CDS             68143..69546
                     /gene="fumC"
                     /locus_tag="ACHKXP_000641"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO7645710.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWIAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69543..70472)
                     /gene="tus"
                     /locus_tag="ACHKXP_000642"
     CDS             complement(69543..70472)
                     /gene="tus"
                     /locus_tag="ACHKXP_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO7645711.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAIFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70548..71849)
                     /gene="rstB"
                     /locus_tag="ACHKXP_000643"
     CDS             complement(70548..71849)
                     /gene="rstB"
                     /locus_tag="ACHKXP_000643"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO7645712.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71853..72572)
                     /gene="rstA"
                     /locus_tag="ACHKXP_000644"
     CDS             complement(71853..72572)
                     /gene="rstA"
                     /locus_tag="ACHKXP_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO7645713.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72701..73036
                     /gene="ydgC"
                     /locus_tag="ACHKXP_000645"
     CDS             72701..73036
                     /gene="ydgC"
                     /locus_tag="ACHKXP_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO7645714.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(73033..73755)
                     /gene="folM"
                     /locus_tag="ACHKXP_000646"
     CDS             complement(73033..73755)
                     /gene="folM"
                     /locus_tag="ACHKXP_000646"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO7645715.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73792..75174)
                     /gene="ydgI"
                     /locus_tag="ACHKXP_000647"
     CDS             complement(73792..75174)
                     /gene="ydgI"
                     /locus_tag="ACHKXP_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7645716.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75360..76304)
                     /gene="ydgH"
                     /locus_tag="ACHKXP_000648"
     CDS             complement(75360..76304)
                     /gene="ydgH"
                     /locus_tag="ACHKXP_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO7645717.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76828..78360
                     /gene="pntA"
                     /locus_tag="ACHKXP_000649"
     CDS             76828..78360
                     /gene="pntA"
                     /locus_tag="ACHKXP_000649"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO7645718.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCTCSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78371..79759
                     /gene="pntB"
                     /locus_tag="ACHKXP_000650"
     CDS             78371..79759
                     /gene="pntB"
                     /locus_tag="ACHKXP_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO7645719.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79784..80818)
                     /gene="tqsA"
                     /locus_tag="ACHKXP_000651"
     CDS             complement(79784..80818)
                     /gene="tqsA"
                     /locus_tag="ACHKXP_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO7645720.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
BASE COUNT        20434 a        20002 c        21149 g        19626 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat
       61 acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg
      121 aatgcttaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaataccc ccaaaggggt
      361 gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc
      541 ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag
      661 cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt
     1081 accaaaggaa atttagttgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa
     1141 ttcgctggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc
     1261 gtaacgacgg aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc
     1381 ggtgcgggaa ctggcgcaat tatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg
     1501 actgaactta ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca
     1561 gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggtggtc cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg
     1741 agtggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag ccaactttgt ccataccacc
     1861 atcttcccga acggtccgaa agattttgaa gataaagacg atggccgtgt gattggtaac
     1921 ttggttggtc tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagttgcat
     1981 cgcgacttta cttattgtta cagcaaaggg gtgttcaagc gtgttctgcc tgctgaagag
     2041 tatgcggaaa ttcactggga tcaactggaa gcgggtgacg ttaatttcat taaagatttt
     2101 tactaccgtc tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc
     2161 atcgcagaac gctggaattt gggtgaagag tactggggct acgcgaaaaa taaactctgg
     2221 tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt
     2281 gttaactgta tgttcaaccg tgactgcatg acacataccc atatcaactt tattggttcc
     2341 ggcttgccat tgaaactgca acgtgaagtg gcgaaagagc tttttggttc tgaagatgcc
     2401 tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgctaagtgg
     2461 tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt gtaactgggt ctggccaatg
     2521 accgtttcgc cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc
     2581 ttcaaagcga tcaccggcga agagatgact caggaaaaat tagatttagc tgcagagcgt
     2641 atctttacgt tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat
     2701 gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact
     2761 gaaggtacgg acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa
     2821 gaaatgggct gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg
     2881 ctggaagata ttgccgtcga tctggcagca cacaatctac tgcctgtgta aggaatgggt
     2941 gagatgaacc atcgggatga attgccgtta gcgaaagtta gcgaagttga cgaggcgaag
     3001 cggcaatggt tacaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct
     3061 gaaatcctgg cagagttcat tcgccagcat tctgcggctg ggcagctggt ggcgcgcgcc
     3121 gtgtttctct cgccacccta ttcggttgca gaagaggaac tgtcagtttt gctggaaagc
     3181 attaagcaga acggagacta cgcggatatc gcttgtatga cgggaagtca ggatgattac
     3241 tattactcaa cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag
     3301 caggacattt gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt
     3361 ccttacaaag tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa
     3421 gccgcgatcg ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca
     3481 tcgacggcgg tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc
     3541 ttatgcgaat ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca
     3601 tggatagggt ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga
     3661 cgaaaatttg ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg
     3721 ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg
     3781 tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag
     3841 ggaagccgtt tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat
     3901 cacttttttc gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg
     3961 acgggggcat cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc
     4021 ggctgcagtt attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact
     4081 aaagtggcag ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc
     4141 atttgcgtca gcgcctgtcc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa
     4201 attcaggcgt ggttgcagga taataaatac tatcaatatc agctacctgg tgccgggaaa
     4261 ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt
     4321 cgcaacatgc tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg
     4381 aattttggcc ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc
     4441 tggggctgca cgccttgctt cgtgctcgtg gcgtgaagaa atcagccacc gatcatggtg
     4501 agaaggttta tctttacagc aaagcggtca gattatggca ctggtcgaat gcgttactct
     4561 ttgtattgtt gctggcaagt gggctgataa atcactttgc gatggtgggc gcaactgcgg
     4621 ttaaaagtct ggttgcggtg catgaagttt gcggattttt gttactggca tgctggctcg
     4681 gctttgtgct gatcaatgcc gttggggata atggtcacca ctatcgcatt cgtcgtcagg
     4741 ggtggctgga acgagcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg
     4801 aagaacatcc tttaccggca acaacacagt ctaaatttaa tcccttacag caggtcgcct
     4861 atgttggtgt catgtatgga ttgctgccgt tgttactatt gacggggctg ttgtgtctct
     4921 atccgcaagc cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt
     4981 ttgctctggc atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg
     5041 ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa
     5101 tgatcattac acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt
     5161 tcctgcgcca tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg
     5221 aaggatatac ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg
     5281 atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag
     5341 agataccatt ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg
     5401 aggataacgc ccgaatcgca accgctgggt atgccgcgca aattgccagt atgggttata
     5461 gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac
     5521 gggttgctgt aaccggtaat tcttcgcgga taagcagcgc tggagatagt agtcggattg
     5581 cgaataccgg aatgcgagta cgcgtttgta cattgggtga acgatgtcat gtcgccagta
     5641 atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagtggcg
     5701 acaatgtaca catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg
     5761 actcaattat tctcgggcca ggaggaagcg ccgcactggt atatcatgac ggcgaaagag
     5821 ttcgcttcgc cgtcgccatt gaaggtgaaa acaatattcg agccggagtc cgctatcgtc
     5881 ttaatgaaca gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac
     5941 gccgacgtgc acgggaggca ggttttacga ccgcccgagc catcgcctca ccaatttctg
     6001 cacatgccgt aagtccctga acgaaaggct ggtcgtgcat taaccagggc aacgcgccga
     6061 acgctacgcg cccacgaata tcttcgggtt gctgtaacgt ataatcttca ccaacatcag
     6121 ggatttcatc ccctgttttc tgtaattgtt cgcgtagtcc atggaaagga atatctttca
     6181 ctttaagcgg acgctgcccg cgggcatcga taaaaacgtc aaacgagtag ctgttgtctt
     6241 ccgttttcag gacggtgcgc gactcattaa tttccatttt gtagtcttca ccgagggcga
     6301 gaatatgaat gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga
     6361 ttgccgcata attatcgatg aaaacccggg caaggccttt actgaaccgt ttatggtcgt
     6421 gttcattcag atgtggaaca atttcctgta cggcttcatg caggcgcaga atgacataac
     6481 gccagggaac agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg
     6541 cccagtcaaa tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga
     6601 cattcaggct ctctaaggct atgcgttgac tccagtctgg atcagcaaac ttgatttcct
     6661 ctactatcaa tctaaatacc cgatccaaaa ggccctcttc gcctttttga atctcagcat
     6721 ttaatgcctg atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg
     6781 cttcgggtaa aatacccgtg cgcgacatca acgtgatatt tagcttttca cttgcgttat
     6841 cgcggtgaaa aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca
     6901 ctgccattgc cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat
     6961 cgacctttgc ttccatcagg cctgaccacg ggctgggaaa atacgttcgg gttgcttctt
     7021 cttcatcagg ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta
     7081 aatcctgatt tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc
     7141 atgattcata aacagccact gcaaattttt gctttcgtgc ctggtctact aatcgtaaaa
     7201 attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg
     7261 tttctttttt aacgccataa cgctggagat ggctggcttc ttgcttttgt agccattcga
     7321 gatacgtaca ataaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg
     7381 agttttcctc atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag
     7441 aaatagaaag tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc
     7501 cggcacccac aatagcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt
     7561 agtgttatca gataagtttg gttgaaattt ttagaatttg agaaatttca gacagggatg
     7621 aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg
     7681 actcagtggc tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagattttt
     7741 cagtcgggca gtgtgttttt ccagataggc aagggcgctt ttttcatcag tgaaaaggta
     7801 gatcccaccg gcttcgtggt tcttttcact ttcggtccat accttccaca gaaaaccagg
     7861 ttcctgatta atcgactcag caagcggttt aagctgctca gccattgtgt cgccaaatgg
     7921 gccattgaaa gaaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac
     7981 gcatacgaag acagtgtaaa tcttttaacg tattggatac agagaagaaa cagagggaaa
     8041 aaggagaaga ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc
     8101 caccatgtca aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat
     8161 tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga
     8221 acgcaacgtt gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag
     8281 aggcaaaacg taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc
     8341 tatattttgt tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa
     8401 cccaataatt cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg
     8461 tggataaatc ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa
     8521 aggaataaat catgggatct gatgcgaaaa acttgatgag cgacgggaac gtgcaaattg
     8581 ttaagaccgg cgaggtcatt ggcgcgactc aacttactga aggcgagtta attgttgagg
     8641 ctggcggaag agctgaaaat accgtggtca cggggaccgg ctggttgaaa gttgcaaccg
     8701 gtgggatcgt caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg
     8761 ccattgccac agatgttgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta
     8821 cggttaatgg ccgccatccc gaaggtgaat tcagcgttga tcagggttat gcctgcggtt
     8881 tgttgctgga aaatggcggt aacctgcgtg tactggaagg acatcgcgcg gaaaaaatca
     8941 ttctcgatca agagggcggc ctgttggtca atgggacaac ctcagcggtc gtggtagatg
     9001 aaggtggtga attgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg
     9061 gcggcgtttt tatgctggcc gggaaagcca gtgatacgtt gcttgctggt ggcaccatga
     9121 ataatctcgg tggtgaagac tctgacacta ttgttgagaa tggatccatt tatcgtctgg
     9181 ggacggatgg ccttcagctc tacagttccg gtaagacgca aaacctgtcc gtgaatgtgg
     9241 gtggtcgggc tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag
     9301 tgatcctgtt gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac
     9361 cggttgaact gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggttatg
     9421 gcgcagagct gcaacaatca acgattactg tacagcaggg cggtgtgttg attctcgacg
     9481 gcagtacggt aaaaggcgac agtgtcactt tcattgttgg taacatcaat ctgaatggcg
     9541 gaaaactgtg gctgatcact ggtgcggcaa cgcatgtgca actgaaagtg aaacgcctgc
     9601 gcggagaggg agcgatttgc ctgcaaacca gtgcgaaaga aatttcacct gacttcatca
     9661 atgtgaaagg ggaagttacc ggggatatac gcgttgagat aacagatgcc agtcggcaaa
     9721 ctctgtgtaa cgcactgaaa ctacagccag acgaagacgg gattggcgca acgctccagc
     9781 ctgcgtaaaa acacaggcgc aacccgtgaa ggttgcgcct tgtctttatt agcgggacgc
     9841 tcgttgcaga atgatggctg acggcagacg ttgcaggaac cgcatacgca gcatcatcat
     9901 aatggctgcc gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg
     9961 ttcaacgacc agatcggtca gtgccagaat atagccgctt ggcaagccca gcacccagta
    10021 agccgtaaag gtaatataga aaatggaacg cgtatcttta taaccacgca aaatcccact
    10081 gccaatcacc tggatcgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc
    10141 agccagcgtt acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga
    10201 aaccgtgaat atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc
    10261 agcggtttgc gcatccagcg ttgagccctg acccagacga taacctacgc ggatagttac
    10321 cgctgccgcc agcgacattg gaagcacaaa catcagtgaa ctaaagttca gggcaatctg
    10381 gtgtcctgcg acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag
    10441 tgtcacttca aagaatagtg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat
    10501 aaccgcagga tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tggagcgggc
    10561 gcgtttaatg taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac
    10621 gccacaacca acgccaccga gctcaggcat accgaaatga ccataaataa agatatagtt
    10681 caccggaatg ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc
    10741 cagaccttca cattggttac gggcaacctg gaagaaaaga tagcctggcg cgccccacag
    10801 caacgcacgc agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga
    10861 gcggataatg taacctgcat tccacagcac cagcataatg agaacggaaa caaaacctgc
    10921 cagccagaaa ccttgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa
    10981 ttgcgcgata accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag
    11041 ccagatagaa gtaccgatag cgaccgccgc catgtcggtg gcactgtagc cgcccgccat
    11101 cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc
    11161 taatgctaat aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg
    11221 tagttatatg agagactaaa aagccgcctg aatgggcagc aaaaagaaga agcaggggaa
    11281 attcagtcta ttgtagcgag gatttattaa ttctccagtg aaaaaatagc cattcttacc
    11341 ccttcactgg taagggcgtt ttccacctgc tattgtgctg aacagaatgt cttaactgat
    11401 ttcaggagtt gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat
    11461 tgacgagaaa ccaaattttc gtacgcatgt ggtggagtta cccgaccaca tgctggacgg
    11521 cctggaaacc ggtgcttccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa
    11581 cggcaaccat gtcagttttg acctgatgaa agaaacgtta cgcattacca atcttggcga
    11641 tttaaaagtg ggggattggg taaacgttga gcgtgcggcg aaattcagtg atgaaattgg
    11701 cggacactta atgtcaggtc atattatgac cactgctgaa gtggcgaaaa tattaacctc
    11761 agaaaataat cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatattct
    11821 gtacaaagga tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac
    11881 gcgtttttgc gtccatttaa ttccggaaac tctggaacgc acgactcttg ggaagaaaaa
    11941 actcggcgca cgcgtcaaca ttgaaattga tccacaaact caggcagtgg tagatacggt
    12001 agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa ccaggtacag aagcctgatc
    12061 tgaaatcgcc cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt
    12121 tttcaacacc gcgtgagaac acgacctgcc agagctgaat atcacgggcg cggaaagcac
    12181 ctgcacaggc attcagataa taggtaaaca ttcgtttaaa gcgttcactg tagttatccg
    12241 caatttctgg ccatgcggcg aggaatcgtt cataccacgc catcaacgta gtatcgtaat
    12301 cagcaccgaa gttatgccag tcttccatca caaagtgggg ttcgctggat tgagcaatct
    12361 ggcgtacaga gggcaggcaa ccgttcggaa aaatatattt gttaatccag ggatcaacat
    12421 tcagatcggt ttttttcgaa ccgatagtat ggagcaggaa tatgccttcc ggtttcaaat
    12481 tacgatccac caccgcaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc
    12541 ccacagaaac aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg
    12601 tgacatccag gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga
    12661 cgcccaccac gcttacgtca taattagatg ccatgtagtg tgccagtccg ccccagccgc
    12721 agccaatatc cagtacgcgc atccctggtt ttaactgcaa tttttcacaa atcattttga
    12781 gcttcgcctg ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt
    12841 gcatgaaggg atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctctttgc
    12901 cgactatcca ggcacgtttt ttactctgca gattgaagag acgagcgccg gcaatacgca
    12961 gcgtgtcttt gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa
    13021 aaaacatatc cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca
    13081 aagagccttc ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg
    13141 gggcagaacc gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc
    13201 agttgtcatc cggtacgctg acttcttcta tacacgatga actcatagtt tctccatcac
    13261 tggtcggtga tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc
    13321 gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacccttgc
    13381 cgaaatgacc atccatagtg agtcgggagc gatcctgtcc ccgtaaaaac ataatattta
    13441 tcgtagcttt aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt
    13501 ttgttagcag cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac
    13561 ttcggcattg ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag
    13621 cgcgaccagc actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat
    13681 actgatcagc caggaaacta ccagacttgc gaggaagcac agacccagtt gtagagtgtt
    13741 ctgcaaagct gcggcgtgac cagttgcgtg tgggaaggga cgcagcgcct gggcgacaac
    13801 aatagggtag atcgcgccat tggcaatcgc catcacacag aatgggatca ggatttcgac
    13861 cagcgacaca tggctaatga agcctgcagc ccaggtcgca atgacgctga cagcaaacag
    13921 caccagcaac cacggtaata actgcttgcc ttgccatttc tgcagtgcgg cgcgacagcc
    13981 ataaccacca atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg
    14041 gctgtagccc atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc
    14101 tgaacaggcc gcatatatca gcacgttgcc gcgataggtt ttagaacgta gcaggtcggt
    14161 aaaggtcaga ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat
    14221 aggcagaatc agcaccacgg taatggcaaa cagggtggcg aaaatcgcct gccaggaaaa
    14281 atggaccagc agccagcttc ctaacagggg agcaagtgcc ggagacagac ccaccagcgg
    14341 catgatggtc gcaaaaatac ggttgacttt ctgtgaagga taataatccg tcactaacgc
    14401 ttgccagata accgccgcgg cgcagacccc cacagcctgt acaaaacgca ataccagcag
    14461 cgtagcggcg ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc
    14521 gattaataac accggtttac gaccataacg gtcggagagt ggcccccaca gaagctgggc
    14581 tgcggcaaaa ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg
    14641 caggtcggcc tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag
    14701 tacgctcaaa cccgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta
    14761 aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt
    14821 aatatttgat gactggtttc aaaaattttg taggcagact atgtggtcag aatattcact
    14881 cgaagttgtt gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagctgca
    14941 tcgcgttcct tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtgcc
    15001 gctctttgaa cgacggcacc gtgatgtgga gctgaccgct gctggagcgt ggtttctcaa
    15061 agaagggcgc tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc
    15121 gaacggctgg cgcggtcagt tagctatcgc agtggataat attgtcaggc cagaacgtac
    15181 acggcagatg atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca
    15241 ggaagtgttc aacggtgtct gggatgcgct ttccgacggg cgcgtggaac tggcgattgg
    15301 cgcaacacgc gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag
    15361 ctggagttgc gttgttgcca gccaccaccc gctggcgttg atggatggcc cattcagcga
    15421 tgatacgttg cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa
    15481 gcgtattacc tggttgctgg ataaccaaaa aagacttgtc gtgcctgact gggaatcatc
    15541 ggcaacctgt atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc
    15601 gtggcttaat gaggggaagt gggtagcatt agaactggaa aacccctttc cggattcggc
    15661 atgttgtctg acatggcagc aaaatgatat gtcgcctgcg ttaacctggt tgcttgagta
    15721 tctcggcgat agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc
    15781 aacgggtgat taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat
    15841 caagcgcgga tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga tacgatccaa
    15901 cagcatgttg aacgctgttt cacccagcga gtcttttggc tgatggatcg tggtcagcgc
    15961 cggcgtaaaa tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg
    16021 gacgcgcaga cccatttcat ctgcagcaca aagtgcgccc attgccatga tatcgccacc
    16081 acagaagacg gcagtagggc gatgcggctg cgacagtatt tgctgcatgg cgcgataacc
    16141 agattcgggt tcaaagtcac cctgaacaat ccagctttcc ggcaccttga tcatcgcttc
    16201 ttccatcgcc ttcataaaac cggcaaggcg gcctgcgccg gtgttacgtt ccagcgggcc
    16261 ggggatgacg ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc
    16321 gccttcgaac gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc
    16381 catcaccacc attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta
    16441 ctcagaacac atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc
    16501 ccgctgtttc tcaagattgt tccacgcatt acccagaatc agggtgtaac ctttctggaa
    16561 gcaatttttt tcaaccgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc
    16621 cagcaaaccg atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga
    16681 gtagtgtaat tctttaatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg
    16741 tgttttgttg attacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc
    16801 ttttattgtt gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa
    16861 ggtaaacgtt tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa
    16921 acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat
    16981 tttggccgat cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg
    17041 agattttttc atcacagtgt gtaaaaatgc gtaatatcac tttttgtgtg gtaataaaaa
    17101 aggagaaaac ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct
    17161 cggtttgatc gtagccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc
    17221 gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt
    17281 ttgttatttt catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg
    17341 taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc
    17401 gattggcgcg tttggtatcg ggacaacgga gttctcgcca atgggcttgt tgcccgtcat
    17461 tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt
    17521 tggcgtaatg gtgggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag
    17581 tgcgttgatt ttcctgatgg caattttcac gctcggcaac gtactttccg ccatcgcgcc
    17641 ggattatatg accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt
    17701 tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt
    17761 tgccactatg tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg
    17821 gttgggtgaa accatcggct ggcggatgtc atttctggca acggcggggc tgggagtgat
    17881 ttcaatggta agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt
    17941 gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc tgacgacggt
    18001 actgggagct ggtgcaatgt ttactctcta cacctatatc tctccggtac tgcaaagtat
    18061 tacccacgca acaccggtgt tcgtcacggc aatgctggtg ctgattggtg tcggattctc
    18121 tatcggtaac tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg
    18181 ctttttgttg ttgttgatgg tgattatgct ggcaatcccg ttcctggccc gcaatgagtt
    18241 cggcgcagct attagcatgg tggtgtgggg cgcagcaacc tttgcggtcg taccgccgtt
    18301 acagatgcgc gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat cagtcaatat
    18361 tggtgccttt aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc
    18421 tgggctggga tacagctttg tgccggtgat gggagcgatt gtcgcgggac tggcattatt
    18481 gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt
    18541 aaacaaaaac gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc
    18601 ttgccgaatg cggtgttaac gactcatccg cgttgacatg aaaagacgcg ttaggcgtcg
    18661 tatcatgcag cgcttgtcgg atgcggcgag tgccttatcc gacctacatc gatgatccgc
    18721 gtaggcctga taagcgtagc gcttcaggca atgctgcatt tgccatcagt tattatgcag
    18781 cgagattttt cgctacgaat tcccagttca ccagcgccca gaagtgctcc agatagccag
    18841 gacgtgcatt gcgatagtcg atgtaataag cgtgttccca gacatcaacg gtcagcagcg
    18901 gagtcgcatc ggtggtcagc ggagtacccg cgttagaggt tgaaacgata gccagtttgc
    18961 catcgctgtt tttcaccagc caggtccagc cagaaccaaa gtttttgatc gctgcatcag
    19021 taaactgcgc tttgaaatcg gcaaagctgc caaaagatgc ggcgatagct tcagcgactt
    19081 ttccagtcgg ttcgccaccg gcgttcggtg ccaggcagtt ccagtagaaa gtatggttcc
    19141 agacctgagc tgcgttgttg aatacgccac cttcagagct gcgaataatc tcttccagtg
    19201 atttaccttc aaacgcggta cctttaatca ggttgttcag gttagtgaca taagtctgat
    19261 gatgcttgcc gtagtgatac tcgatagttt ccgcagaaat gtgcggtgcc agagcatctt
    19321 tagcatatgg tagtgcaggt aattcgaatg acattgctac tctcctttat tattaatttg
    19381 catacgtaca atagccttat tgtgcgtatg gatagggtag caaattaaaa ggtgagacaa
    19441 aagatgaact taccctgttg ccgtaacaac agggtaaagt tttagcgaag tgtttttggg
    19501 gtcattaccc gacgagcgcc aacatagtgg cgctgccagt agtcttcact gagagaagtg
    19561 atttgaattt cctgacctgt gcgcggtgac tgaataaatt tgccgttgcc gacatacacg
    19621 ccgacatgat cggctgtgcc gcgtccctga gtacggaaaa agaccaggtc gccgtttttc
    19681 agttcactac gttcgattgg cgctgcatca cgcaggtgat acatttcatt cgccgtacgc
    19741 ggaatacgaa ttttcaccaa atctttataa gcgtaataaa ccaggccgct gcaatcaaaa
    19801 ccggtacgcg gtgagctgcc accccaacga tatggcttac caatttgctg catcagttta
    19861 ttcattgcca ctttcgtagc tttttgcacc ttcgctttgt gcgcgtcggc aagagttcct
    19921 gacgcggcat ttttgacttt cacacaatgc gatttatagc ccttacgttt ggtacacttt
    19981 tcggtaaagc tggctgaggc cgttttattg acggcgtgtt tgctgcgagt tttggcgttt
    20041 ttagaagcgg taatggaaga ttttttaacg gaagaagcgg ttttgctttt ggtcgtactt
    20101 ttactggcgg cttttttcgc tgttttctgg gtctttttgg tggttgccgt gcttttcttt
    20161 ttatctgctt ttttggtgat atgagtggta gcagaactct ccctggcttg ctttgaagca
    20221 tgggccatag gcgttaaagg cagggtggta aaaagtaaag cacagagcgt gatcgaaata
    20281 cggtttatcc gcgccactga gcgtccctct gttaaggcag gtctatcatg cctgcgtctg
    20341 atttacaaaa cttgatgatt ctaatgcata gaagaagaga aagttaatag cttttattgt
    20401 taaaaaatac gtttcgttgg cgagcgcaaa aaaatgcagc agtgaaagct aaaaatggtt
    20461 ataaatacgc cagtttgccg gcatcagaga agaaacgaat atttgaaatg caacaaatag
    20521 agcaaaggga taaaatagca aaagcgtgat gaaaatgtta ttttccgatg ccgcactgac
    20581 ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca atgagcacca
    20641 ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac atgaaaggtt
    20701 caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt gccgcatgtg
    20761 gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg gaactgccga
    20821 aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg gtcggcggtt
    20881 gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc aaagaaactg
    20941 ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa tactgacgga
    21001 tgcgaacggg catccgtcag tcctttctct atccccaatc cagcccttgt ggcgaacttg
    21061 aaaatcccgc ctcacgcagc agcgtaaaca tcggcgtttg ccggaccggt agatcattca
    21121 cttctgttag cgtaaagcgc agccgtggtt cgcgacgcag tgcggtagtc agcgcgtgga
    21181 aaacctctgg ggcgagtaat tcctcttttt cctgccagac cagcattttt ttgccacctt
    21241 gcgccagata gagtaacaat ttgccgccag aaacgaccac cagcgcaccc gcccgacgcg
    21301 ttggaaccag cgttgccgga tgtgcaggcc agggcagaag atttccccac acattagccg
    21361 gatcgttggc agagagcgcc actggtgtat agtggcgcgt ttgcgtggct tgtgtcgcca
    21421 gatcgcgcaa tcggtcaata gtcagacgtt cagcgaattg cgcgccaccc agaccttcta
    21481 caaaacgacc tcgcataatt cgcccggagt cttccatact tcgacaaagc gtttgcatcg
    21541 atggaaaccc gccagggata ttttcggcta tcaccgcctg acgactgatg atgccgtagc
    21601 ggtcgagcat attttccgcc agcgccagca tcctttcggt atcgtttagt ggctccacct
    21661 gcaataacga ccagcgtcca gccagatttg gtgtgttgta agataccagc ggcgagacgg
    21721 gttgcgcata gacaggacgt ccacgacgag cccggtgact gcggcgagtt gaggtgcgtg
    21781 tgttggaact gctgcgggtg agggcgcgta acggtgccca aatgtcgctg gtgatgacgc
    21841 cttgccagac cagcgcccat aacgcctctt gcagggcaga gagatcaacc gattcgccga
    21901 ttttgtcgcg tatccgttgg ctgatttgtt gtgcaaacca ggctcctccg tcagccagaa
    21961 cagcgactat cgcttgttgc agcgccgaac gattcgcctg atccgcttcg gcgggagtga
    22021 acgattctgc agcatattcc tgtagatgca gtgccaccag gccgtcatct tcacccagtt
    22081 ttttttgccc cgaccagata accgcaccgg ttgccagtaa ttcatcgagc atttctggcg
    22141 aatagtcgcg tacgcgggca ggcaggatct ggctttccca gagtgaggcg ggtaaaccga
    22201 ctccggcaag ctgttcgatc acccgcatca cgccatctac gccctcataa acgcctggcg
    22261 atgttgaggc aaagagcgcc gggctaccat cggtggcggg taatacgccc tgacgttcca
    22321 gcagcaatcg cgcataggtc ttggctgcaa cgggacgcgt cgcttctctg gcggcttgca
    22381 gcgagcgcaa acgcagacga cgaaatactt catcgctcac ccagatgtct tgttgcagat
    22441 tcatcaccag accctgttca cgcagttgct gaagctgctc ttcgacaatg gcaataccga
    22501 gactaaactc atgagccagt tgttcagccg tgaccagagc atgagcccgg agatagcgca
    22561 gaaagaggtc gcgaagcggg taactgactc tatgtaaata aatctctggc aatgactctg
    22621 gtagtcgtac gccgagggca tcacgcagcc tggcggcatc atccatacag gcaagacgct
    22681 cctgtccgct aatcatcgct gggaagattc gttttactgc aagaagattt tccagatacg
    22741 acgcaacctc ttcactgctg cctgtatgcc gttgcgccag gtcttcaacg gtcattggcc
    22801 ccagttcgcg cagcaggtcg aacaatcctt cttcaccttt cgctcttctg ccaggagcca
    22861 gtcgttgcaa ctcttcttcc acctggcgaa tgacctgcgg gtcgagtaat tcccccggat
    22921 cgacctgtcc gagtagattg cgcagtaact cgctgtccag cgacagtacg gatgcccggc
    22981 gctctgccag cggggcgtcg ctctggtaca taaattccgc gacatagccg aacaataaac
    23041 ttgtggcgaa aggcgagggc gtagtggtcg ttacatcgga tatttgaatt tcgccaccgt
    23101 tcaggcgacg catcaaacgt tccagtgcgg gaagatcata aacatcttgc agacattcgc
    23161 gtagggtttc gagaatgacc ggaaaatccg gatatccctg agcgatttcc agcaactgac
    23221 tggcgcgcag ccgttgttgc caaagcgggg tgcgatggcc cggagtgcgc cccggcatta
    23281 ataatgcccg cgcggcgcat tcgcgaaaac gggcggcgaa aagtgccgag ctgcctaccg
    23341 cctcgcggac aatttgcagc aacttttctg gttcaaacaa aaaaatcgcg gcgtcgggca
    23401 atttaccatc agtgtcagga atgcgcgcaa caatgccgtc atcactggcg accacagacg
    23461 cgtcagcgcc ccatagggca tgtattcgcc cggcaatcgc cagcgcccag ggttcatgca
    23521 cccgtcttcc atagggagag tgcaaaataa tacgccagtc gccaatttca tcacggcacc
    23581 gttcgaggac cagatggcga ctccccggaa cgatgcccgt cgcgttgcgt tgctcgtcaa
    23641 tcaacccctg aatattggcg ttcgtatttt cttctgccag ccacggggga atagtcccgg
    23701 aaaagaacgc gccatccgcc agtaaatgaa gaaaatcgcc gatcatctcg cctaattcag
    23761 ccggacgtcc gttaccttca ccacgccaga aggggagccg ggcagaacga cccggagcag
    23821 gagtcacaat cacctgatcg cgggtgattt gctggatccg ccatgaggta gcgccgagag
    23881 tgataatgtc gttcacccgc gactcatata ccatctcctc atccagttca cccacccgcc
    23941 gcgaaccggc ctgttcttca ccttcgggta ataacacgct atacatgcca cgatccggaa
    24001 tggtgccgcc gctggtaacc gccaacaatt gagcgccagg tcgggcggtc aatatcccgg
    24061 tctccctgtt ccagaccagt ttggggcgaa aagcagaaaa atcgccagag ggatagcgcc
    24121 cggaaagcat atccagcgtg gcgtcaaaaa cacgtcttgg cagatctttc cacggtgcgg
    24181 cacggcgtac gcgggagtac cattcgtcta cctgtaatgc atccatcgcc gcggcggcaa
    24241 cggtttgctg cgcaaggacg tcgagtggat tatgcggcgg tgtcaggttt tccagcctgc
    24301 ctgcgaacat acactctaca atgactgcgg aatcgactaa gtcacgccgg gtacgaggga
    24361 aaaacagccc tttagataca ccgccaacct gatgtccggc gcgaccaatg cgttgtaacc
    24421 cactggcaac agaaagcggc gttgccacct gaatcaccag atccaccgcg cccatatcaa
    24481 tccccagttc gagactggag gttgcgacca cgcagcgtaa ttcacccgat ttcagcgcct
    24541 gttcggtgat tgctcgttgt tctttagaga cggagccgtg gtgtgagcgg gcaataaaaa
    24601 cgtcgctact ttgtacatgg ttagaggttg cgccggaggt cgactcgaaa tgggccgtat
    24661 caacggcgat agacggggaa cgctgtaagc gtgcggcgta aagctcattt aatcgtgccg
    24721 tcagtttttc cgccagccca cgtgaattag taaagacaat ggtcgagcga tggcgcaaca
    24781 cttcatcaag gatacccgtt tcaatatatg gccagatgga gccttcccgg ccggcatggc
    24841 tgtcttcgcc ggtgccgctg gcgaccgatg agacatcatc catattggcg accggtacga
    24901 caattcgtat ctgcggatgg cgcattgcgg gcgggttgac taccgtaacc gggcgatcgc
    24961 caccaagaaa tgctgccaca tcgctggctg agcgcacagt ggcagaaagg ccaattcgct
    25021 gtgctgaggt gtggagcagc gcatcgagcc gctccagact taacgccaga tgcgcaccac
    25081 gtttactgcc cgctaccgcg tggacttcat caataattac cgtttcgacg ccgcgtagcg
    25141 tttcgcgcgc gcgggaggtc agcatcagat agagtgattc gggtgtggtg atcagaatat
    25201 ccggcggatt acgggtgagt ttgctgcgtt cctgtgcagg cgtatcgcca gtacggatcc
    25261 ctacgcgaag attgacttcc gtttcgccgc gccgccgccg ttcatcggca atacccttca
    25321 acgggagctg caagttgcgc tgaacgtcgg tgcccagagc ttttatcggt gaaatataga
    25381 ggatgcgtga ggttttacgc ttatgcgcct cgcgggtatc ttcgccgcct tcgcggaaga
    25441 gccgatcgag ggcgtagaga aatgctgcca gcgttttccc ggagccggtc ggtgcaatca
    25501 ccagcgcatg ttcgcttcgc gccgccacat gccaggtttg cgactggaca gcggtcggct
    25561 gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga aaacacgtcc ggcaggagcg
    25621 atgaagggtc tggattatct gccattaacg ctattccccg tgaatatcat gcttgatgcg
    25681 tggaatagcc tcactataga tgcctgaatc attgcatgtc atcaggcatc gactcgatta
    25741 cacctcttcg gcggcaggta gcggccagcc tcccagacgt ttccagcggt tgacgatttc
    25801 acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc
    25861 gaagtccatg ccagcggtct ggcaagcctt tgacaatacg gtttgtccga gtgccagccc
    25921 ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac gggttacgtt tcagtgaggc
    25981 gcgttctgcg gcggccatca taaagctgtg atcaaaattg gcattgtgcg ccaccataat
    26041 ggcgcggtta cagccgctcg ctttaatacc tttacgtaca actttaaaaa tttcgtgcag
    26101 cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc gggtcaatgc cgttgaaggc
    26161 gagggcctct ggctgcaaat ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt
    26221 gtccggcatc agccagcctt gttcatccat tttcagggtg atggcggcaa tctcaagcag
    26281 cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg atcacaacag gataaaaacc
    26341 acgaaaacgg tcgcacagac cggtaagttg agcgttatcg gacatctgat tctcttaata
    26401 cggataaatt gcagcgcgca ttatgacaaa tattacttgt cgatgcagta aagatgcggg
    26461 cgcgatgagt tcacgcccgg caggagatta gttgcccaga ccgcgaccgg cgtctttctc
    26521 ttcgattaac tcaattttgt aaccgtccgg atcttccaca aacgcgataa ccgtagtacc
    26581 gccttttacc ggacccgctt cacgggtcac gttacccccg ttttgacgga ttttttcgca
    26641 cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca taagcagtgc cgagttcgta
    26701 tttatccacg ccccagttgt aggtcagttc aatcaccgct tcttcggttt ccgggccgta
    26761 gccaacaaac gccagtgagt atttgtattc cgggttttcg ctggtacgca gcagtttcat
    26821 gcccagcact ttggtataaa aatcgatgga gcgttgcaga tcgccaacgc gcagcatggt
    26881 atgaagaaga cgcatctttt tatcctcaaa atggctaaca gattcaaaat gttgttttag
    26941 tatagcggcg gctttacgcc gctcgcaatg ttggattaca acgtcgggta atcggtatag
    27001 ccttccgcgc cgccaccgta gaaactttcg gcacgctgtg ggttaagctc agctttgcgc
    27061 tgcaagcggg cgaccagatc cgggttcgca atccagtcac gaccaaatgc caccgcatca
    27121 attaaccctt tgccgatcag cgtttcagct ttttctactg tgtatgcacc tgcgccgata
    27181 atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg catcagtata cggttcaccc
    27241 cccgcccaat ctggttctga catatgcaga taagcaatgc cgcgtttacc cagttgttca
    27301 atcagataca gtgcatcggc ttcttcattc gcgccgttat ccgtgttctg gaaagtaccg
    27361 attggcgaaa cgcgaatgcc aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc
    27421 acttccagta ccagacgtgc gcgattttcc acgctgccgc cgtactgatc ggtacgatgg
    27481 tttgaagaag gagaaaggaa ctgatgcagc aaataaccgt gagcagagtg gagctctacc
    27541 agatcaaaac cggcttcacg cgcgttagca atggcctgac ggaaatcatt gacgatacct
    27601 ggaatctctt ccagttcaag cgcacgcggc atggatgttt caacacggat cgcctgacca
    27661 ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg ctgaaggcgc taccggtgcc
    27721 tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg tgtgccacag ctgcacggcc
    27781 atatgaccat tttcagcatg aacgccagcg gtgatttttt tccatgcggc aatttgctcc
    27841 ggactatgga tgccaggcgc acctgcatat ccttttgcct gggcagaaat ttgcgtggct
    27901 tcactaataa tcaaaccggc actggcacgt tggcgatagt attccgccat caacggggta
    27961 ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg gtgccataaa aatacggttt
    28021 gccgccgtga tcgcgcccac tttcagtggg gaatacagtt tttcagatga cataaaggct
    28081 cccggtcgtc tggtgataaa aaatgcctgc taaacggcag gcgtcgcaat aatgtttttt
    28141 acatgggcca gcgcgttttc cagtggctcg aaactgcgcg aaattttggc ctgcagattc
    28201 gcacccagcc atagtgcgta aagcacttgt gcctgttgta gcggttcgcc acaaaaggtt
    28261 aaacaatggt tctcacggcc attttccagc gcctgagaga gcagggcgat cacgccgcga
    28321 gcacctttat ccatcgcgct gcgcatatct tctgacagat cgcacacttc ggcagagagt
    28381 tttactgtca ggcaaccact gatggttcca tgttggcaaa actggttcag tgtttgctgg
    28441 taataagcca gtatgcggtc gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt
    28501 cgctggtgat atgcggcgta atgacgctca agcatggcaa cgccaaacgc ttctttagag
    28561 cgaaagtagt gatagaagga ccctttcggc acttcagcgg tttttagtaa ttcgcttagc
    28621 cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg
    28681 cgagtatcat gttcggtgtg tttgttcatg cgttggagta tagtagacca gtcggtctag
    28741 tgcaagcaaa gttgctactt tggacattaa cagcttcaat aatggtaata atcttatctg
    28801 aggagggcga agtggcggag caattagagt tctttcccgt ccagagcccg tgccggggaa
    28861 tttgccagtc tgacgaacgc gggttttgtc gcggctgttt tcgtagtcgt gatgagcgtt
    28921 ttaactggaa taaaatgagc gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc
    28981 gattaatgcg caaattacgc gcaaataagc cagcgtcatc agacgaaccg gagcaaccat
    29041 cactcttttg aacctaatct tgcgtatact cctctcatat ctgataagag gaagcgatta
    29101 tggttcagcg tattactatt gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct
    29161 actggcgatt gatggactgg aatatgtccg cccgccagct ggtcagtttt attgaagagc
    29221 atctggatct cggcgtgacc accgtggacc atgctgatat ttatggtggc tatcagtgcg
    29281 aagcggcgtt tggcgaggca ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg
    29341 tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa cgtcattggt cattacatca
    29401 ctgaccgcga tcacatcatt aagagcgccg aacagtcgct aattaatctc gcgaccgatc
    29461 atctggattt gctgttaatc caccgaccag acccgttaat ggatgccgat gaagtggcgg
    29521 acgcgttcaa acatctgcat cagagcggca aagtgcgtca ttttggcgta tcgaacttta
    29581 cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt tacccttgcc actaatcagg
    29641 tggaaatatc cccggtgcat cagccgttac tgctggatgg cacgctcgac caactacaac
    29701 aactgcgtgt tcgtccgatg gcgtggtctt gccttggtgg tggtcgtctg tttaatgatg
    29761 attatttcca gccgctgcgt gatgaactgg ctgtggtggc agaggagtta aacgcgggct
    29821 cgattgaaca ggtggtttac gcctgggtat tacgtttacc atcgcagccg ctgccaatta
    29881 tcggttcagg taaaattgag cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga
    29941 cccgtcaaca atggtttcgt atccgtaaag cggcactggg gtacgacgta ccgtaagcac
    30001 ttttaggaat agccgccgtt caaaaatgtg tcactggttt acacttattc aggaatgcac
    30061 aatgaacgga ggtcctatga aacgttttag tctggctatt ctggcgctgg ttgttgcaac
    30121 cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc gtcacgtcgc aaggggtagg
    30181 gcagtcaatt ggtagcgtca ccattactga aaccgataaa ggtctggagt tttcgcccga
    30241 tctgaaagca ttaccccccg gtgaacatgg cttccatatt catgccaaag gaagctgcca
    30301 gccagccacc aaagatggca aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc
    30361 acaaaatacc ggtaaacatg aagggccaga aggtgccggg catttaggcg atctgcctgc
    30421 actggtcgtc aataatgacg gcaaagctac cgatgccgtc atcgcgcctc gtctgaaatc
    30481 actggatgaa atcaaagaca aagcgctgat ggtccacgct ggcggcgata atatgtccga
    30541 tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt
    30601 aagaggccag cgtaccttgc ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc
    30661 gccagacaat tgccgccagc tcactggcgg caggttgatg atgacgggca aggctgtcgc
    30721 aaatccgctg taattcttca agtgtggccg ccagtgattt ttgctgaacg ccacgctcac
    30781 tcatcactcc ccgcaacagt gaaatacagt tatcccgtac tcgcgataac ggatcggaac
    30841 gcgattccca gtcgcgcaat tgccagacaa catgagaaca gttcagcagc actacacccc
    30901 agcgtaataa ccaacggcga gccagcgcat cctggctgtt actcaactga ctgacgtgat
    30961 gataagtgag cgattcaaat tcgctttcac tcagtgttgg atggcggctt agctgatcga
    31021 caaaatcccg gcgcagcgcg cgaatatggc ggcggctttt acgagcatcc gatcctggac
    31081 gcagaatggc gaacgctaac cacgccaacg cgacgccaac gatttttgcc agattatcgt
    31141 taagaaaatc agcaaaatca tacaccgggg gattagtgac agcgataaaa gaacccataa
    31201 aaacaatcag ttgcccccac aatgcggcaa attttggcat ctgcaattta agaagctgca
    31261 ttgtcgccag cagtggaaag agaaacagta aaaattgcca cagatcgcta atctggacca
    31321 tcagaccaaa tttgaccaca aagctgaata gcgaaagtaa caccagcgtg cgcatcagaa
    31381 gtgacaacga cttaaacggt gctgcgacgg cggagtagag tacgcagcta attgctgcca
    31441 gcgttaatgc attggcaccg gcatcccatt gcgaagcaat actccatgcg ccaatcatca
    31501 tcaacctaca aaatgtacgc agcccgctcc acatagcttc ggcgttatcg gtatgacgag
    31561 ccaggccgga tgtccgtggg agtctggtat gatcatctac accgctttgc agacgatgta
    31621 attcctgact actttgcaga taaaggcggc aaaaataacg tagtcgctgc cagaaggcga
    31681 cgtgccgata gtcggcgacg ttggtcgggc gtagcggggc gataatacgt gcgacggtgt
    31741 aaacatctgt ttgcgaactg tcgagcgccg tcagcaactg ttcgagaatt tctcgtgtgg
    31801 cacctggcgg tgagggccag ttgagcaaca tacgtcgcag gctggagatg acactggtca
    31861 tacgtaattg ctggtggagc agcgcattaa ggcgcgcgtt ttgctggcga aaacgatagt
    31921 ggctccagaa agcctggata cgcagcaaat tcatggtcag tatctgccca atcacccctt
    31981 catgtgctgc acgaatggca tcggttgttt caggctgcca gagtaaactg gcatgttcaa
    32041 gtagtcgggc gtgcatgttt ttcaatgcgg ttaaaagggc tgtagcatcg gaactgctag
    32101 gcaggatcat catcatcatg ccgccgcaca aaatgccgac aatcacctcg caaacgcgcg
    32161 cctgagcgat atcccacagc tggctggcct cagtaatatt aaccatcgga aaggcgatga
    32221 tggcagccgt gtagcccgcc agttgaaatg catacgcgac gttattcgtg aagtgcgcac
    32281 aggcccaggt acaaaagcca agccacgccg acatgctcaa tagaaaaaac cacggctcat
    32341 tgagcgtatg cccggcaaga agcagtgccg caatggctcc gagcaaactg ccagcgatgc
    32401 gtccgaggct tttgctgata acaccgccaa cggtgggaaa gctaaccact gcagccgagg
    32461 tcatcgccca atagggttca tccagattta aataataggc aaccgtcagc gccagacaca
    32521 tggcaatggt attgcgtaac gcataacgcc attgcgccag cgtggccctg aaccagggca
    32581 aattgcgcaa ggaccatgac gatgcgttca ttattgacct acggcaatgc tacaggtggt
    32641 gcccgacacc aacgtgacat cgcctgggac tttatccagc gcaaagcgga cgggaacgcg
    32701 ttgggcgagt cgaacccagg ggacgttagg tttaacatcc ggaataaggc ttgagtcact
    32761 ttcaacgctc tgatcataaa tcgcccgacc gatactggaa acgtgaccct gtaacgtttt
    32821 gttatcacta tagagggtaa tttgtgctgg tgcaccttcg cggatatggc gcaacttggt
    32881 ttcttcaaaa taaccaatga cgtaaaacga gtggctgtcg acaagggcaa acagcggttt
    32941 cccggtatca gcgtaatcgc cgatgcgggt agtaaggtta gtcacccatc cactcaccgg
    33001 ggcgcgaatt tccgtttgcg ccagttgcca ttgtgcctgt ttcagcgtcg cctgtgcggc
    33061 atcaacactg gcctgcatcg ctttaacatt gaggttagcg gtatcgagtt cttcggcaga
    33121 aataaaattt tgtgagagat gacgacgacg attcgcttcg ttattggctt ttgcgaggtc
    33181 agattgcgct ttcgccagct gagcctgtgc gttcagctcg gcgatctgaa agggcgtttt
    33241 atcgatcgtg agcagaagat cccccgcgtt aaccagctgg ttgtctttta tattcagctc
    33301 aacaatacgt cccgacacct gtggggtgat agaaacctgt tctgcgcgta tttttccgtc
    33361 gcgtgtccac ggagattgca tgtaataatt ccacagccac catccggcaa gaaccgcaac
    33421 taccgctaca atgattgttg agaaatactt aattgtttta attgacatag ttaccacgca
    33481 atcagtattg ccagagcaag gcaaacgcaa atcgcaaaca gcgataaatc cattaacaag
    33541 ggatgccaga tgtcaccggc gtagatccag ccacgaagca ggcggtgtac gacaagccag
    33601 atgacgaatc caaacgcgaa tgctttgaaa aacggaggaa agtagacgga cgcaccgaac
    33661 accagatctt gtaagggcaa tcctgttgca ttgagcataa acttcacaaa gtagattcct
    33721 ttacgaccga atatattgcg ttagattaat aaatattctt taagtgcgaa aaatttacgc
    33781 gcaatttctg aaaaatatgt ctgacggtaa ccaaatgcag caatacattt gttttagcaa
    33841 tacaattgct gcacactatt ctaaaagccg cataatatct tagcaagcta attataagga
    33901 gatgaaattg gaatcgccac taggttctga tctggcacgg ttggtgcgca tatggcgtgc
    33961 tctgatagac catcgcctga aaccgctgga gttaacacaa acccattggg ttacgttaca
    34021 caatatccat cagttacctc cagaccagtc gcaaattcaa ctggcaaaag cgattggcat
    34081 cgagcagcca tcactggtcc gtactctgga ccaactggaa gaaaaagggt taatttcgcg
    34141 tcaaacttgt gccagcgatc gtcgggctaa acgtattaaa ctgacggaaa aggcagagcc
    34201 gctgatcagc gaaatggaag ctgttattaa caaaacccgc gcggaaatat tacatggcat
    34261 ctccgcagag gaactggagc aactgattac gctcatcgca aaacttgagc ataatatcat
    34321 tgagttacag gccaaagggt gaaatgaagg gggcgtgtgg tcagtaacct gaccacacgc
    34381 aaacttattt agcgcggaga aacggtcacc tgactgccat tgctggccag tacgacacgt
    34441 tggcccggag agaaacgagt gttgccttgt ttctgtacca ccatgatagt attaccatcg
    34501 tctttacgaa tttccagctc gacaccctgc gttttgttca ttgcactctg tacgccctga
    34561 ccagctacgc cacctgcaac agcgcctgct gcagtagcca gagaacgccc ggttccgcca
    34621 ccaacagtat tccccaggaa accaccaaga acagcaccgc caattgcacc gataacgttg
    34681 gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa cgatggtgcc atagctgaca
    34741 ttctgtactt gtttcgcttc agaagcggta taaacatccc ctgacagggt gtcgttatta
    34801 acacaaccga caagagacag acctaccatt gaaacaacca atacgcgttt aatcattgaa
    34861 aaatctcctg ttcaccatga aacgccacgc aagcatccct catggtcaaa gtatatggca
    34921 tatgtgaatc catggttaca tgttctacca aatatgagca aatcataatg aaagtaggat
    34981 caaccaatta taaacaaaac attcatgatg attattgatt tgaagataat gttttactac
    35041 caacggtaaa aatcggtcat tccgcatggt attcacgaaa agtttatgtt tgagtggtgc
    35101 gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt
    35161 caggcaccag ccttgatggt gttgatgttg tgttggcgac aattgatgaa caccgggtcg
    35221 cacagctggc aagtttgagt tggccgatcc cggtatctct gaaacaggct gtactggata
    35281 tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact caactcgggc
    35341 gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaatctgcag gcaagagata
    35401 tagttgcgat cggttgtcac ggtcaaaccg tctggcatga accgacgggc gtggcaccac
    35461 acactttaca gattggcgat aacaatcaaa ttgtggcacg caccggaatt acggttgtcg
    35521 gtgattttcg ccgtcgcgat attgccttgg gaggacaagg cgcaccgctg gtacctgcgt
    35581 tccatcatgc gctgctggct cacccaaccg agcgacgaat ggtgctcaat attggcggca
    35641 tcgccaatct gtcactgctc attcctgggc agccggttgg gggctacgat accggtcctg
    35701 gtaacatgct gatggatgcc tggatctggc gtcaggccgg taaaccttac gataaagatg
    35761 ccgagtgggc acgggcgggt aaagttattc tcccactgct gcaaaatatg ctcagcgacc
    35821 cgtatttctc gcaacctgca ccgaaaagca ccggacgcga atactttaac tatggttggc
    35881 tggagcgcca tttgcgccat tttccgggtg ttgatccccg agatgtgcag gcgacactgg
    35941 cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc tgcgaacgat
    36001 tgatggtatg tggtggaggt agtcgtaatc cgctactcat ggcgcgtctg gcggcattac
    36061 tgccaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat gacatggaag
    36121 cattggcttt cgcctggctt gcctggcgga cgctggcggg attaccagga aatctgcctt
    36181 ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccccgct aacccgtgat
    36241 atcagagtta actgaatttt tcttaccgcg tctgccgtta cacttggaac gttagggagg
    36301 gcgtattgcc ctccagacca ggaaagtctt cgggatatga ctatgaaaaa actgttaatt
    36361 atcattttgc cggtccttct ctccggctgt agcgccttta atcagctggt tgagcgcatg
    36421 caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
    36481 ccgcgccagg aggtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcatt
    36541 tcagcctctg gcgcgcgtta cactgacgga atctatgttt tctggtcgaa aggcgatgaa
    36601 gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca aaatccacag
    36661 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
    36721 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
    36781 aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg aacgttggct
    36841 ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg ctaccgtgga
    36901 tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg aaaaaggcat
    36961 ggtgttttac accaacctcg gcagccgtaa agcacatcaa atcgaaaata atccgcgcgt
    37021 tagcctgctg ttcccgtggc atacccttga gcgccaggtg atggtgatcg gtaaagcaga
    37081 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
    37141 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
    37201 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
    37261 cggttttcgc gtcagccttg aacagattga gttctggcag ggtggtgagc atcgcctgca
    37321 tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc ttgcaccctg
    37381 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
    37441 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
    37501 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
    37561 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
    37621 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
    37681 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
    37741 ctgattggcg acccgagctt caaagctgcc gagcgtaagt tgaacaccga agaaactgtt
    37801 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
    37861 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
    37921 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
    37981 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
    38041 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg gtgtggtgct gcaaattggt
    38101 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
    38161 aatcaggtgt ttggcctgac cgttccgctg atcactaaag cagatggcac caaatttggt
    38221 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
    38281 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
    38341 atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg taaagcaccg
    38401 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
    38461 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
    38521 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
    38581 gacctgatgc aggcactggt cgattctgaa ctgcaacctt cccgtggtca ggcacgtaaa
    38641 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
    38701 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg taaaaagaat
    38761 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
    38821 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
    38881 ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg gcgcgaacgt
    38941 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
    39001 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
    39061 acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg gtgaacatat
    39121 cctcggtatc gtccgtcagg tgaaagccgc gaatccgcag gcgaaatatt tttgcgatcc
    39181 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
    39241 gcggcacggt ttgcctgcca gcgatatcat tgcgccaaat ctggttgagc tggaaatact
    39301 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattgc
    39361 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
    39421 ttttgaaatg ctgctggtca ccgccgatga agcctggcat atcagccgtc cgctggtgga
    39481 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
    39541 actgcttcag ggggcaacgc tgcaggaggc gctggaacat gtgaccgctg cagtctacga
    39601 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
    39661 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
    39721 gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga cagcgcgtct
    39781 tgtacttccg gacgttcagc catacgttgc ataaatgctg caatgtgctc taacccttcc
    39841 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
    39901 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
    39961 ttcttctcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc agggcgaaac
    40021 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
    40081 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
    40141 tactgcataa tcgctacgcc ttccgtcagc aaagtaccgt catccagcag caatgcaggc
    40201 acctgcccct tagggttaac ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
    40261 aaatccacac tgacgagggt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
    40321 agagagcagg cacccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
    40381 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
    40441 taaaagagtt tccctgaatt ttacgctacg gctgctttcg ccgctttgtc tgcagcgtca
    40501 tcctgcgtca tgcggtgcag tttcggcgcg gtcagcagca tcagtactgc aataacggca
    40561 gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat cagcggatcg
    40621 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
    40681 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttga
    40741 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
    40801 cttgcgacca gccagcttac agacacgata ccagcgtcag acgcgaattt cgcacccagc
    40861 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
    40921 ggcagggtat cgcccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
    40981 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
    41041 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
    41101 aagataatgg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
    41161 ttcatcgcga aggcttcttt accgaagata accacgatac cgaaggcaac aacgcccagc
    41221 gccatacgcg caacttcctg attgtgcagc agccaggtgg cgatagcgat cagtgccaca
    41281 acaccaataa tggtcagcag caggttacgg tagttgatag gctcgaagtc tggttttgaa
    41341 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
    41401 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggcgtagca
    41461 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
    41521 agacgcgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
    41581 aggccgttac cgaccgcaat agccgccata cccatataaa cgataccggc gtcgtgacca
    41641 gaccaggcaa ccaaagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
    41701 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
    41761 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
    41821 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
    41881 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
    41941 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
    42001 gctttctcgc gttatgtata aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
    42061 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
    42121 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
    42181 aacgtaacgg cagatttttt cactgcactg agaggggggt aatggcttaa cggcgatatg
    42241 ttctgctatt tagcgttttt actagtgaga tagtccagtt tctgaaaaat agccagtgta
    42301 atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa atgattttaa cctgattctt
    42361 gcaatgtttt tacattgcga cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
    42421 ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta cctgatgata
    42481 acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat aaagaaaggt
    42541 tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct ctttgtattc
    42601 acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc aggtataacg
    42661 cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgctggaa ccactttcag
    42721 tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac gattacaaac
    42781 gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt ttaatacgac
    42841 gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat cttccggaac
    42901 ctcgccatta tgctgctcca gcaagatacg gcaggttttg atgatatttt ctgctttgct
    42961 gttataaagc ccaatcgttt tgatataggt tttcacccct tcaacgccca gttcaagcat
    43021 cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa cactgacatc
    43081 ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac tgaaattaag
    43141 ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca ggcgttttgc
    43201 tttattcatc agacattccc tgtttcaccg tttggcaatg cacgttctgc agctgcttca
    43261 gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat cagtcccagg
    43321 ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt gtggaaaatc
    43381 tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc aaacaatgtg
    43441 ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt tgcgcccata
    43501 ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc gaaggcttca
    43561 gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc taatgattga
    43621 tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac cgaggcgatg
    43681 atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg
    43741 gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag agcgttagtg
    43801 gcagtggacg tgaccgccaa cagaggacaa aggccgagca actggaccag cgcagagttg
    43861 tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta ttctccacag
    43921 gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc gcgttttacc
    43981 gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa atcaccacca
    44041 tctttcttca ccgcccagtg cgcatcatct gcaccactga tttttttacc cgcaaaatgg
    44101 gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa gccctggcgt ttcgtggtgc
    44161 tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg ctcccaccag cagctgaatc
    44221 gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg tttgtcatcc
    44281 tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt tatcagatag
    44341 caactctgtg ccagcgcatt gttatagcgt tcggctggca gcacctgatc aaataacgcc
    44401 ttttgttgca gactggcctg ttcagcaatc gtcgttttgg tcatctggtt gatggccgca
    44461 gttaaccctg ttgaacccgc tgcaaatagc gccaacgtaa tgccgtgttt tcggatagtt
    44521 ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc
    44581 agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg atagccgccg
    44641 aaactgcgga tcaaccagac taataagccc gcaagcgcgc cgaaaataag acgaccacga
    44701 ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga atgcgccgag catggtcgct
    44761 ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac
    44821 attgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg aatcgctttc
    44881 tgccatagca accatacgcc gccagccagc caggcgagat ttacccattg ccaaccagcg
    44941 cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga atgaccggca
    45001 cggacagagg ttttaaatgt atccagcggt gtcgcctgac taatgccatc aatacctaag
    45061 cgtagtgtgt tcatatcacc accactggcg gtatgaccgc taaaaataac ctggatggcg
    45121 tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc
    45181 acggggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa cgggttttgt
    45241 cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc cagcacgacc
    45301 atccaccatg gcgcgagggg gggaatactt accgccagca ataagcctgt cagcaatgct
    45361 gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac gagagcttcg
    45421 gctaacagag cactaaccga tgccaacagg atctgaacga gagtacccca accaaaaaac
    45481 cacagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat aatgcgcgat
    45541 gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat ttagtcctcg
    45601 tttacaacct tctgctgggc ggcttttttg gcctgagcgc gggcaatagc cgcggcaact
    45661 gccgctttgc gcggatcaac ctgttcttct ggtaccgcat tagcctgttg ctgttccagc
    45721 ttgcgtgctt tggcgcgtgc aatagccgct tcgacggcag ctttgcgcgg atcaacctgt
    45781 tcttctggct ccgcattagc cggttgctgt tcccgtttgc gcgctttggc tcgggcaata
    45841 gcggcttcga cggcggcttt gcgcggatcg acctgttgtt ctggctcggc attagcctgt
    45901 tgctgttcca gcttgcgtgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgc
    45961 ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt gcgcgctttg
    46021 gcacgggcaa tagcggcttc gacggcggct ttgcgcggat cgacctgttg ttctggttcc
    46081 gcattagcct gttgctgttc cagcttgcgc gctttggcgc gggcgatagc tgcttcaacg
    46141 gcagttttac gtggatcagc aacggttgct gcgtcgttag tttgctgcag ttctgcctgt
    46201 ttcgctctgg cttgcgcttt acgggcttcc cgtgctgcaa taattgcact gttatccggg
    46261 cgttcgcccg ctttaatcac aataggctgt gtagcctggg cctgtttctc tttcacccgc
    46321 gccagagcgg cagcaatcgc atctttatct ttggctgcag gttgaacggc tgcgctctta
    46381 tgtcgttcaa ggcgagccgc tttttcgcgc tccagacgag cctggcgcgc ttcgaaacgc
    46441 gctttggctt ctgcggcgcg cttttcttcc tgacgaatag ccgcaatttc agctttttcc
    46501 tgacggaaat attgcaccag gggaatattg ctcgggcaaa cccacgcgca agccccacat
    46561 tcaatgcaat cagcaatgtt atgcgtggta gctttatcgt gttgctgacc tttgctgaac
    46621 cagtacaact gttgcggcaa aagatccgca gggcaggcgt cagcacaggc gctacaccgg
    46681 atgcagcttt gttcttcctg tggttcgcca agttcattgg cagagggagc caacagacag
    46741 ttggtaatct ttacgaccgg gacatccagc catggcaagg taaagcccat tagcgggcca
    46801 cccataatca ccatttgatc ggcagagggg cagaatccgg catcattcaa taaatgacgc
    46861 actggcgtcc ccagccgtgc ccagacgttg cccgggcgag cgattgcttc gccagtcagg
    46921 gttacaacac gctcggtaat cggctcgcca tcaataacgg cacgtttcac tgcataagca
    46981 gtgccgacgt tttgcattaa tacgccgata tcggatgaac gcccgccatg tggaacctgc
    47041 ttcccggtca gaatgtaggt taattgttta gcaccgccag aaggatattt ggttggaatc
    47101 acccgcagag aaatatcgtt agagtccgcc agcaccgcgc gcagcatgga aatcgcctgc
    47161 ggtttgttat cttcaatgcc gataagaatt tcgcgtggct gcagaatatg cgcaagaatg
    47221 cgaataccct ctacgacctg agccgcgcaa tcctgcatca aacggtcatc ggcggtaatg
    47281 tacggctcgc actcagccgc gttgataatc aacgtttcaa tcttatctcc gccaccctgc
    47341 aatttaacgc ctgtcgggaa tcctgcaccg cccagcccgg caacaccaaa ctgatgtatg
    47401 cgctcgatta actcttcgcg actgcgagtg cgataatcgg cccagccgtc gcgcgggatc
    47461 cagcagtctt caccatcggc atcaataatc acgcttaatt cagctaaagc tgaaggatga
    47521 gccgtagagt ggggcgcaat agccgtaacg gtacccgagg tgggcgcgtg aacaggcagc
    47581 attttgccgc gaccacgggt aagtggctgg ccgcgcaata ctttatcgcc gacgctaacg
    47641 cacaactcac cttcagcgcc aatatgctgt ttcagtggaa taacaaaacg ctgcgccagg
    47701 ggtacctggc gcaggggtgt accgttggac tgggttttca tctccggtgg atggatgccg
    47761 ccgttgaaat cccagatttt attttttctg aatgcagaga ataacttaag catggtgttc
    47821 cacgggaatg atacgcacgg gaatggtgtt cagatcccat ttccaggagt caggtgtttc
    47881 tgcgaccggt tgcaacgaga tgcagtgcgt cgggcacgga tcaacacata aattgcagcc
    47941 cgtacagaga tcactcatta ccgtatgcat ggcacgggta gcgccaacga tggcgtctac
    48001 cggacacgcc tgaatacatt tagtgcagcc aatacagtta ttttcatcaa taaccgccac
    48061 catccgcgca ggcgttatct cttgcgcttc gccatccagc ggctgcggct cgacattaag
    48121 caactcggca atttttagca tcacagcttc gccacctggg gcgcaacggt tgattttttc
    48181 accgttacag ctgatggctt ccgcgtaggg gcgacagccg ggataaccgc actgaccaca
    48241 ctggctctgc ggtaagattt cgtcaatttt ctcaacgacc ggatcgtctt ccaccgcaaa
    48301 acggcgggag gcataaccca gaatggcgcc aaacgccagg cccagcaggc tcacggcggc
    48361 aacggcaatc cagatagcat tcattacaac ttcaccaaac cactaaagcc cataaaggcc
    48421 agagacataa gacctgcggt aattaacgca atggcattac cgcgaaaagg tgccgggaca
    48481 tcagccacag caaggcgttc gcggatggcg gcgaagagca ccatcaccag cgagaaaccg
    48541 acagcggcgg aaaaaccgta cagcgccgac tgcaagaaat tgtgcccgag attgatattc
    48601 agcaacgcca cgccgagcac tgcacagttg gtggtgataa gcggcaaaaa aatccccagc
    48661 agacggtaaa gcaccgggct ggttttgcgc accaccatct cggtgaactg cacgaccaca
    48721 gcaatcacca gaataaatgc cagggtgcgc aggtaaatca gattaagtgg gatcaaaatc
    48781 cacgtatcga taagccaggc gcaaatagac gccagcgtca tcacaaacgt tgttgccagc
    48841 cccatgccca tcgcggtttc cagttttttg gaaaccccca taaacggaca gagaccgaga
    48901 aacttgacca gtacaaagtt attgaccagt acagttccga caaacagtaa caggtagtca
    48961 gtcatttttc ggcctgaaat aaaaaagccg cctattatcc gttaatccag agcaggcgac
    49021 aacaggttaa ctgtaaggtt attacggatt cacaaacgtc gcttttaccc gcgacgagcg
    49081 tttgaaatag gggacgatca atgcggttgc caacaaagta aacagcaact ggcgaacggc
    49141 tatgccatct tccacgggtg aaaaagcgaa tgcttttact gccagcaata cggaaataag
    49201 cagccagata atatagtgtt taggaacaca acgccgacgt ttaaagaaag cgatggtcaa
    49261 ccacagtgtg taataccaca aggcgatagc ggtaataaaa gaaacgcccc ataagatttg
    49321 tgtcgtcagt gcttgtccgc cgagcgtttg aaatgtttgc ggagaagata acgcagcggt
    49381 atacagcaac aacgccagcg tcgtactcaa cagagcgact aaaagccagg ccaacgggcc
    49441 aagcaaccag cctccaatac gttgtggcgt tgtggtggtc ataggttctc cagaatagcg
    49501 acgcaaacag gcaaatctaa ttttaggggc gttattataa ggcttttata accgatcgct
    49561 actttcttta ttggacatag tgccagacgg acttcggtac ctggccgagg tcaaacaaac
    49621 gcccgccaga aaccagctct gcgcgacggt gatcggcagc acgatacata ttgatcagtt
    49681 cctgatcgtc cgtcagggtg taattaagat ggtcgtagag tttttccaga ctttcgagtg
    49741 aactgatttt gcgaaatttt aataagtagt cctgaacagt cataaaaggt ccataaaata
    49801 attaatacca aaggggtagg tattattagt gatgataaaa tttcaccaaa agtgaacgct
    49861 tgtgttatta caataataag aatgcgctga tggcaaatat tcagggatga aaaactgctc
    49921 tgaaaagagt aacgcgcttt actatccctg acaatcacca acaacatcga acaagataat
    49981 aaattcctgg tttaatatcc gacaagtgaa aacatgcacc cggacgggca gcatgtcgct
    50041 ccacaagtgc agagcttact tgtgttgtac cgaagcactc tgttcaggtg gctgatagtt
    50101 gtcaatgtga ctcgccacgc caagaagaat gactgaaacg acaagaacga tccaacctgt
    50161 taattcaata agacgattca ttacagccca catctctctt gattgatcca ttaacttcag
    50221 ggggtaaatg ttacttagca ataatagccc cagcaagatt tttactgagg tttttgcgat
    50281 atttagcttt tgtcgttgga aaattcgcta tttttttgac ttaagttaaa caacatccct
    50341 tattgctggc aggttattaa actgttgagc gtgggtaaag gatagtgtca aatagccatc
    50401 atacttcaat gagaggcaat gacatgagcg acaacatccg tgttgggttg attgggtatg
    50461 gttatgcgag caaaaccttc catgcgcccc tgattgcggg cacgcccggg ctggaactgg
    50521 cggtaatctc cagcagtgat gaaacaaaag taaaagccga ctggccaacg gttacggttg
    50581 tctctgagcc gaagcatctg tttaacgatc ccaacataga cctgattgtc attcctacac
    50641 ccaacgatac ccatttcccg ttagccaaag cggcgcttga ggcgggtaaa catgtggtcg
    50701 ttgataaacc ctttaccgtg acactgtcac aagcgcgaga gctggatgcg ctggcaaaaa
    50761 gcctggggcg tgtgctgtct gtattccata accgtcgctg ggatagcgat ttcttgacgc
    50821 taaaaggttt actcgcggaa ggcgtgctgg gtgaagttgc ttactttgag tctcattttg
    50881 accgcttccg tccgcaggtg cgcgatcgtt ggcgtgaaca gggcggccca ggcagcggta
    50941 tctggtacga tttagcacca catcttcttg atcaggccat tacgcttttt ggtttaccgg
    51001 tcagcatgac ggtagatttg gcacagttac ggcccggagc gcagtcgacc gattatttcc
    51061 acgccatctt gtcctatcca cagcggcgag tcattttaca cggtaccatg ctggcagccg
    51121 ctgagtcagc acggtatatc gtgcatggat cccgaggcag ttatgtgaaa tatggcctcg
    51181 atccacagga agaacgtctg aaaaatggcg agcgtctacc gcaggaagac tggggctacg
    51241 atatgcgtga tggcgtactt acccgcgtgg aaggtgagga acgtgtcgaa gaaacgctgt
    51301 tgacggtgcc tgggaattat ccggcttact atgcggctat tcgtgatgcg ttaaatggcg
    51361 atggtgaaaa tccggttccg gcaagccagg caatccaggt aatggagttg attgagctgg
    51421 gcatcgaatc cgccaaacat cgcgcgactt tgtgccttgc atgaaaaatg gatggtgcat
    51481 ttgcaccatc cattttgtta cttcgcggcg actttttctc gcagtgcgcg tttttcctca
    51541 gcgctgagga aagccatttc cagaccatta atctgtgcct ggcggatttg ctcgcgggat
    51601 aacccagcag ctggcgcggc aacggtatat tcgtgaatga tatccactcc ctgtacgccg
    51661 ggatcgtcag tgttaatgct ggcacgaatg ccatgctcaa ggaacgtttt cagcggatgt
    51721 gcagccagct ctgctacggt gctggtctga atattggagg tcagacagga ttcaatacca
    51781 atttgttgct cggcgagaaa atccatcagc gcccgatctt caatggcttt tacgccatgt
    51841 ccaatacgct ccgcacccag ttcacgaatc gcctgccaga tgctttccgg cccggcagct
    51901 tcgcctgcat ggacggtaat atgccagccc gcatcacgcg cgcggttgaa gtgagaaagg
    51961 aacagacttc ccgggaaacc aagttcatca ccggctaaat caagtgcggt aatctggtca
    52021 cggtgggcta aaaaggcctc cagctcttgc tgacaggcgg cttcgccgaa ggttcggctc
    52081 ataatgccga taagcttcgc ctgcacacca aaggtgcggc aaccttcacg tacgccatcg
    52141 atcaccgctt cgacaacacc cgctacaggc agctgatgtg ccattgccat gtagcctggt
    52201 gaaaaacgca gctcgacata gtgcaggccg tgacgggctg catcttcaat gttttcaaat
    52261 gccacgcggc ggcaggcatc aagagaggcg agaactttaa cgccccagtc aagtttggtc
    52321 agaaagctca ccagatcggg ttcgttggca atgacctgaa cgtggggaat cagtgtttcc
    52381 agggattgtg caggaagcga gatattatac tggcggccaa gttcaagaat ggtctgggga
    52441 cgaatgttgc catcaaggtg gcgatggata tcagttaatg gcagggtggt atcaatcatg
    52501 gtcgcactct ttttctggat ataaaagtgc gcaccattat aaaaacaaag cagggtaaaa
    52561 atccacgatt gcgcaacgat tttttacgtt gcgcaattgg ggttaacgaa cagcgcggat
    52621 ggcgttaatt aatccagcca cacctttttc cagtttcgaa cgtgggcagc cggcattgag
    52681 tcggacaaaa ccacgacctt cttcaccgta ggtatacccc ggcatgatcg cgaccttctc
    52741 ttgctcgata agtgcttttt gcaacgcgtt gtcgtcaata tttaacggac gtaaatcaag
    52801 ccaggccaga taagtggatt gcgggatctg ccagttgagt tcaggaaacg cggcgttcat
    52861 tttatctgcg atatacgtca gattatcttt cagatagatg cgtaaggcat ccagccacgg
    52921 cgcgccttgc tgataggcgg cgatatgggc agttaacgcc agtaccgaag gggaagaaag
    52981 cccatcacgg cctttcagtg ccgataaata ggcatcgcgg ctactgctat tttctataat
    53041 cccataagca ccggtcagcg cgggaatatt gaaacttttc gatcccgacg tcagcaacgc
    53101 ccagtctccc cgcgccacat tactccaggg aatatgcggc tgctcgcccc agaccatatc
    53161 catatggatt tcatcggaaa taacccgcac accatgacgc tcgcacaggt cagccatgat
    53221 ctccagctca tcgcacgtcc acactttccc ggtaggattc tgtgggctac acaggagcat
    53281 aattttacat tctggtttcg ccaacacggc ttccagcttg cccatatcgc aaaaccaacc
    53341 gtcagcctgc ttctctaaag caacgggcat tactgtgcgc tggttacctt caatggcctt
    53401 gtaaaatgcg tcataggcgg gtgtgtggat caccacgcct tcacctgttt cagaccactg
    53461 acgaatcagt tctgaaacca tatagataac agaaggccca tacaccaccg actgcgtatc
    53521 aattgcggtg taatgctggg tggaaaacca gtgggcaata gccgcgagaa actcatcgtt
    53581 tttccagcgg ctgtagccaa atacgccgtg catcaggcgc tgattcagcg cctcgataat
    53641 gcagggggca gtggcaaaat ccatgtctga aatcgtgaac ggtaacaggt cagcagtgcc
    53701 gaaacggtca gcgacataat cccactgcgt gcaccatgtg ccatgacgat ccacgacctt
    53761 tgaaaaatcg aacatatctt atccttatgc ctggacagta tgcatcagac cggccatttc
    53821 atcttttact gactgaactt gtggcccgat aacaacctgc aggttatgtt gattaagttg
    53881 tactacgcca attgcccgat tgtccttcag tgcctgcaca ttaacaagcg acatatcttt
    53941 cacagacaaa cgcagacggg taatgcagtt atcgaggctg acaatattgt cggcaccgcc
    54001 taatgcttcg aggattgccg gaacgttgta gcctgattta cccggcgcac cggcaacggc
    54061 tttttcgatt gaactggcca cttcgctatc gcgccccggg gttttcagat tgaagcgggt
    54121 gatagcgaaa cggaagatga tgtagtaaac gacaaaccag attgccgcca ccactggcac
    54181 catgtaccac ttggttgaca gaccatgcaa aataccgaac accacgaagt cgatgatatt
    54241 accgtcggta ttaccgatgg tgacgccgag cacagacatg acggtgaagc cgaggccggt
    54301 taacagcgcg tggatgacat acagaactgg cgctacgaac aggaacagga attccagcgg
    54361 ttcggtagtg ccgccaacga cacaggcgat caggccagaa atcagcagac ctttaatttt
    54421 atggcgattt tccgggcgcg cgcagtgata catagctaac gctgcacctg gcagaccgcc
    54481 gagaaacgca ggcattttgc cttgcgaaag gaaacgcgtg gcgctttcag aaaaaccgtg
    54541 agtggtcggg caactcaatt gcgcctggaa gatggtcagt gcgccgctga cggtttgacc
    54601 gcagacttcc tgcgtgccac ctgcgtcggt aaagcgaatt aatgccacca gaatgtgatg
    54661 cagaccaaac ggcaacagca gacgttcacc ggtaccaaac agcatcggtc cgagatcacc
    54721 cgcgctattg atcatatggc ctaagccgct aatacccatg gcgaaaatcg gccagactaa
    54781 tggaatcacc aggccgacaa ggcccatcac cagcgaggag ataattggta caaagcgcgt
    54841 accgccgaag aatgccagcg catccggcag gcggatatta tggaatcgct catgcagcat
    54901 ccagacgata ataccggcga tcaccgcacc gaggatcccg gtatcgatcg actggatccc
    54961 aagaatgctc tgtatgttat tggctttcag aacggcggcg tctgtggttg gcagaatgcc
    55021 tttattggtt aaccagaagt ttaccgcgag gttcattacc gcataaccga cgaagccagc
    55081 gaatgccgct acgcctttgt tttcgcgtgc cagacccagc gggatggcga tacagaacat
    55141 gactggcagg aaactaaaag caaacgagcc aatcttactc atccaggtaa agatagcctg
    55201 caacacgggg ttgcccagga ccgggagcag ggtgatgaca tcgtggctac taagggaact
    55261 accaatgccg agcataatgc cgcagaacga caataatgcc acgggtaaca tgaaggtttt
    55321 gcctaactgc tggaagaact cccacagcgt gactttcggt gctgttttcg ccgtcataaa
    55381 acgactcctc gtagagaaca atctggattc agtaaattgc gcgagaagat aaaacgtttt
    55441 atcaaatttt agtgaggcat aaatcacatt acgcaacgat aatagcgggt ataagataaa
    55501 taaaaggtaa aacgttttac ctgtcacata atcagggagt aggtcatctg catggctacc
    55561 gccaaaaaaa taaccattca tgatgttgcg ctggctgcgg gcgtgtcggt aagtaccgtt
    55621 tcgctggtgc ttagtggcaa agggcgaatc tctaccgcca caggagaacg cgttaacgcc
    55681 gccattgaag agctgggatt tgtgcgcaat cgccaggcgt cggcgctgcg cggcgggcaa
    55741 agcggcgtca ttggtttgat cgtccgtgat ttatctgcgc cgttttacgc cgaattgacg
    55801 gccggattga cggaagctct ggaagcgcag ggacggatgg tttttttgct tcacggcggt
    55861 aaagacggcg agcagctggc acagcggttt tcactgttac tgaatcaggg tgtcgatggt
    55921 gtggtaattg ccggggctgc aggaagcagc gatgacctgc gacggatggc agaagaaaaa
    55981 ggtatcccgg tgattttcgc ttcccgtgcc agttatcttg atgatgttga tacggttcgc
    56041 ccggacaaca tgcaggctgc acagttgttg acggagcatc tcattcgcaa tgggcatcag
    56101 cggatcgcct ggctgggagg gcaaagttcc tcattaaccc gtgcagaacg ggtggggggc
    56161 tattgtgcaa ctctactaaa atttggcctg ccgtttcaca gcaattgggt gctggagtgc
    56221 acttccagcc agaagcaagc cgcggaagct atcacggcgc ttttacgcca taacccgacc
    56281 atcagtgccg tggtttgcta taacgaaact attgcgatgg gggcatggtt tggtttgctg
    56341 aaagcagggc ggcaaagcgg ggaaagcgga gtcgatcgtt actttgagca acaggtttcg
    56401 ctggcggcat ttaccgatgc gacaccaacc acccttgatg atatacctgt tacctgggcc
    56461 agcacgccag cgcgggaact tggtatcaca cttgcggatc gcatgatgca aaaaatcacc
    56521 catgaagaga cgcattcacg caatcttatt attcccgccc ggctcattgc ggcgaaataa
    56581 tcctctcttt atctgctata cctggtagtg tcctttcctc aaggttaatg ataaaaatgt
    56641 ggcacacagg cggtgccttt tatttttact gaagttcaca ggaggtttat gtgtttaatt
    56701 ctgacaacct gagactcgac ggaaaatgcg ccatcatcac aggtgcgggt gcaggtattg
    56761 gtaaagaaat cgccattaca ttcgcgacag ctggcgcatc tgtggtggtc agtgatatta
    56821 acgccgacgc agctaaccat gttgtagacg aaattcaaca actgggtggt caggcatttg
    56881 cctgccgttg tgatattact tccgaacagg aactctctgc actggcagac tttgctatca
    56941 gtaagctggg taaagttgat attctggtta acaacgccgg tggcggtgga cctaaaccgt
    57001 ttgatatgcc aatggcggat tttcgccgtg cttatgaact gaatgtgttt tcttttttcc
    57061 atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg cggtggcgtt attctgacca
    57121 tcacttctat ggcggcagaa aataaaaata taaacatgac ttcctatgca tcatctaaag
    57181 ctgcggccag tcatctggtc agaaatatgg cgtttgacct gggtgaaaaa aatattcggg
    57241 taaatggcat tgcgccgggg gcaatattaa ccgatgccct gaaatccgtt attacaccag
    57301 aaattgaaca aaaaatgtta cagcacacgc cgatcagacg tctgggccaa ccgcaagata
    57361 ttgctaacgc agcgctgttc ctttgctcgc ctgctgcgag ctgggtaagc ggacaaattc
    57421 tcaccgtctc cggtggtggg gtacaggagc tcaattaata cactaacgga ccggtaaaca
    57481 accgtgcgtg ttgtttaccg ggataaactc atcaacgtct ctgctaaata actggcagcc
    57541 aaatcacggc tattggttaa ccaatttcag agtgaaaagt atacgaatag agtgtgcctt
    57601 cgcactattc aacagcaatg ataggcgctc acctgacaac gcggtaaact agttattcac
    57661 gctaactata atggtttaat gatggataac atgcagactg aagcacaacc gacacggacc
    57721 cggatcctca atgctgccag agagattttt tcagaaaatg gatttcacag tgcctcgatg
    57781 aaagccatct gtaaatcttg cgccattagt cccgggacgc tctatcacca tttcatctcc
    57841 aaagaagcct tgattcaggc gattatctta caggaccagg agagggcgct ggcccgtttc
    57901 cgggaaccga ttgaagggat tcatttcgtt gactatatgg tcgagtccat tgtctctctc
    57961 acccatgaag cctttggaca acgggcgctg gtggttgaaa ttatggcgga agggatgcgt
    58021 aacccacagg tcgccgccat gcttaaaaat aagcatatga cgatcacgga atttgttgcc
    58081 cagcggatgc gtgatgccca gcaaaaaggc gagataagcc cagacatcaa cacggcaatg
    58141 acttcacgtt tactgctgga tctgacctac ggtgtactgg ccgatatcga agcggaagac
    58201 ctggcgcgtg aagcgtcgtt tgctcaggga ttacgcgcga tgattggcgg tatcttaacc
    58261 gcatcctgat tctctctctt tttcggcggg ctggtgataa ctgtgcccgc gtttcatatc
    58321 gtaatttctc tgtgcaaaaa ttatccttcc cggcttcgga gaattccccc caaaatattc
    58381 actgtagcca tatgtcatga gagtttatcg ttcccaatac gctcgaacga acgttcggtt
    58441 gcttatttta tggcttctgt caacgctgtt ttaaagatta atgcgatcta tatcacgctg
    58501 tgggtattgc agtttttggt tttttgatcg cggtgtcagt tctttttatt tccatttctc
    58561 ttccatgggt ttctcacaga taactgtgtg caacacagaa ttggttaact aatcagatta
    58621 aaggttgacc agtattatta tcttaatgag gagtccctta tgttacgtcc tgtagaaacc
    58681 ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga tcgcgaaaac
    58741 tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc aattgctgtg
    58801 ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc gggcaacgtc
    58861 tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat cgtgctgcgt
    58921 ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt gatggagcat
    58981 cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc cgggaaaagt
    59041 gtacgtatca ccgtttgtgt gaacaacgaa ctgaactggc agactatccc gccgggaatg
    59101 gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt ctttaactat
    59161 gccgggatcc atcgcagcgt aatgctctac accacgccga acacctgggt ggacgatatc
    59221 accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg
    59281 gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt tgcaactgga
    59341 caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt
    59401 tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat ctacccgctt
    59461 cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg
    59521 ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat
    59581 aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa ctcctaccgt
    59641 acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg
    59701 gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg
    59761 ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg
    59821 cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg
    59881 tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca
    59941 ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg
    60001 ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt
    60061 tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa
    60121 gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg
    60181 gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt
    60241 gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag
    60301 gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag
    60361 aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc
    60421 tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aatcaacaac
    60481 tctcctggcg caccatcgtc ggctacagcc tcggtgacgt cgccaataac ttcgccttcg
    60541 caatgggggc gctcttcctg ttgagttact acaccgacgt cgctggcgtc ggtgccgctg
    60601 cggcgggcac catgctgtta ctggtgcggg tattcgatgc cttcgccgac gtctttgccg
    60661 gacgagtggt ggacagtgtg aatacccgct ggggaaaatt ccgcccgttt ttactcttcg
    60721 gtactgcgcc gttaatgatc ttcagcgtgc tggtattctg ggtgccgacc gactggagcc
    60781 atggtagcaa agtggtgtat gcatatttga cctacatggg cctcgggctt tgctacagcc
    60841 tggtgaatat tccttatggt tcacttgcta ccgcgatgac ccaacaacca caatcccgcg
    60901 cccgtctggg cgcggctcgt gggattgccg cttcattgac ctttgtctgc ctggcatttc
    60961 tgataggacc gagcattaag aactccagcc cggaagagat ggtgtcggta taccatttct
    61021 ggacaattgt gctggcgatt gccggaatgg tgctttactt catctgcttc aaatcgacgc
    61081 gtgagaatgt ggtacgtatc gttgcgcagc cgtcattgaa tatcagtctg caaaccctga
    61141 aacggaatcg cccgctgttt atgttgtgca tcggtgcgct gtgtgtgctg atttcgacct
    61201 ttgcggtcag cgcctcgtcg ttgttctacg tgcgctatgt gttaaatgat accgggctgt
    61261 tcactgtgct ggtactggtg caaaacctgg ttggtactgt ggcatcggca ccgctggtgc
    61321 cggggatggt cgcgaggatc ggtaaaaaga ataccttcct gattggcgct ttgctgggaa
    61381 cctgcggtta tctgctgttc ttctgggttt ccgtctggtc actgccggtg gcgttggttg
    61441 cgttggccat cgcttcaatt ggtcagggcg ttaccatgac cgtgatgtgg gcgctggaag
    61501 ctgataccgt agaatacggt gaatacctga ccggcgtgcg aattgaaggg ctcacctatt
    61561 cactattctc atttacccgt aaatgcggtc aggcaatcgg aggttcaatt cctgccttta
    61621 ttttggggtt aagcggatat atcgccaatc aggtgcaaac gccggaagtt attatgggca
    61681 tccgcacatc aattgcctta gtaccttgcg gatttatgct actggcattc gttattatct
    61741 ggttttatcc gctcacggat aaaaaattca aagaaatcgt ggttgaaatt gataatcgta
    61801 aaaaagtgca gcagcaatta atcagcgata tcactaatta atattcaata aaaataatca
    61861 gaacatcaaa ggtgcaacta tgagaaaaat agtggccatg gccgttattt gcctgacggc
    61921 tgcctctggc cttacctctg cttatgcggc gcaactggct gacgatgaag cgggactacg
    61981 catcagactg aaaaacgaat tgcgcagggc ggataatccc agtgctggcg cgggaagaga
    62041 tatttacgca tgggtacagg gaggattgct cgatttcaat agtggttatt attccaatat
    62101 tattggcgtt gaaggcgggg cgtattatgt ttataaatta ggtgctcgtg ctgatatgag
    62161 tacccggtgg tatcttgatg gtgataaaag ttttggcttt gccctggggg cagtaaaaat
    62221 aaaacccagt gaaaatagcc tgcttaaatt aggtcgcttc gggacggatt atagttatgg
    62281 tagcttacct tatcgtattc cgttaatggc tggcagttcg caacgtacat taccgacagt
    62341 ttctgaagga gcattaggtt attgggcttt aacaccaaat attgatctgt ggggaatgtg
    62401 gcgttcacga gtatttttat ggactgattc aacaaccggt attcgtgatg aaggggtgta
    62461 taacagccag acgggaaaat acgataaaca tcgcgcacgt tcttttttag ccgccagttg
    62521 gcatgatgat accagtcgct attctctggg gggatcggta cagaaagatg tttccaatca
    62581 gatacaaagt attctcgaga aaagcatacc gctcgacccg aattatacgt tgaaagggga
    62641 gttgctcggc ttttacgcgc agctcgaagg tttaagtcgt aataccagcc agcccaatga
    62701 aacggcgttg gttagtggac aattgacctg gaatgcgccg tggggaagcg tatttggcag
    62761 tggtggttat ttgcgccatg caatgaatgg tgccgtggtg gataccgaca ttggctatcc
    62821 cttttcatta agtcttgatc gtaaccgtga aggaatgcag tcctggcaat tgggcgtcaa
    62881 ctatcgttta acgccgcaat ttacgctgac atttgcaccg attgtgactc gcggctatga
    62941 atccagtaaa cgagatgtgc ggattgaagg cacgggtatc ttaggtggta tgaactatcg
    63001 ggtcagcgaa gggccgttac aagggatgaa tttctttctt gctgccgata aagggcggga
    63061 aaagcgcgat ggcagtacgc tgggcgatcg cctgaattac tgggatgtga aaatgagtat
    63121 tcagtatgac tttatgctga agtaaaaaat aacgccggag agaaaaatct ccggcgtttc
    63181 agattgttga caaagtgcgc gttttttatg ccggatgcgg cgtaaacgcc ttatccagcc
    63241 tacaaaaact cataaattca aagtgttgca ggaaaaggta ggcctgataa gcgtagcgca
    63301 tcaggcaatc tctggtttgt tttcagatga aaacgccgga gtgaaaattc tccggcgttt
    63361 tggccgtgaa ttactgctgc ggaattgccg gtacagccgg aacgttaaga gctggcatcg
    63421 caaacatgcc aacaaaatct tctaacgaca ttttctgccc atttaacgtt atctgaccgt
    63481 tagcatattg caggctggtg gtgatggtat tgtcctgcaa ggtggtcaga cggaacatct
    63541 gccccattgc tgatgcacct tcaacttgct gtttcgccag ttttttcgct tgatcttcct
    63601 gataaccttc cagcttcgct acctgagtca taaactcagt tgccatatcc accggaatgg
    63661 tcagtttcgc atccagagat ttaaccgaac gatctacttc ctgcgccagc gtttgcggcg
    63721 cttctttagt cgttgccgga tctttcagga acagcgacag attcagggca ctttcaccct
    63781 gactgttttt ccagcttagc ggcgcgatag taatcaccgg atcgcctttc agcatcagcg
    63841 gcagggcgct aaagaaggct tccgtcactt tctcctgata aagttcgggg ttgttggcaa
    63901 tttctggctg tgccagcagc gcctgagttt gcgcgttata ttgctggcta aactgatgcc
    63961 aggcttcacc atcaatctgg ccgactttta aagtcagctt gccgctgccc agatcctgat
    64021 tctgtacctt caggctgttt agcgagtaat ccagttggct attgatcgtt ttaccgtcat
    64081 tgaccagatc cgatttaccg ctgatctcca tgccttccag cagtgccagt tctttgcctt
    64141 ccactgaaat ggtcattttt tccagtgaca gtttttgatt tcctacacgc tcaccaaaac
    64201 ttgccagcgt gctggaaccg tcggttttca gattattaaa ggtcaactgc actttctggt
    64261 tgtattcgtt aactgcgtct atccgaccac tttgcgcctc cccggaaagg gagatggctt
    64321 tgccgtctct gtcagcattt aactggaact cgccgccgct aaaggcgact ttttcatcct
    64381 tttgctcgta attcagtggc ttgagcgaaa tatcggaact ggaatcaccg ctgtaaccaa
    64441 tgcgcgagtt aatctcaaaa ggcgtttcac cttttgccat atcaaacagt ggtttgctta
    64501 cttcgttatt aaccagcgtg gtttgaattg atgccatcga cgggatcagg ttcagttttt
    64561 taagctgggc aagcgggaag ggaccatgat caaccgattc gttgaagatg acgctctgac
    64621 cgcttttaat ccacggattt tctttcccgg caatgggttt caccaacagt tgcaactggc
    64681 tgctgaatac gccgcgatga tagttttgat aactcacttc caggttggat tcaggagctg
    64741 tcagtttgag ttgcgcgttc gcctgcgcga ccatgtcttc gagatgggtt tcaatcttct
    64801 tgcctgtata ccatgcgccg cctgtccaga ctacgcctag cgcaacaatg acgcctaccg
    64861 ctaccagcga tttattcata atgattatcc ataaaatgaa atcaggcgga ctggccgcct
    64921 gaaggtgtta taagccttta ataagcttag caagagatgt taattttttc agtaagctct
    64981 tacagcttgt tgtaaacacg cgctaaacgg ccgtggcctt tgacagtcac cggtgattcg
    65041 ttggcggcaa taaacgctga ttcacccggt ttaagctgta actgctgaga acctttccac
    65101 aacgttgcat cgccttcgac gcagaacaaa atggcggcac tctgctggct aatggtggtt
    65161 tctttatcac taaggtcatg cagcgagaag gcaaaatcat ccactggaat cgggaagtcc
    65221 agttctgcac cttgtttcac cggctgggtc aacaactggt tagccggttt ggcttcgaat
    65281 ttcacattgg caaccagttc cggaatatca atgtatttag gcgtcagacc cgcacgcagc
    65341 acgttatcgg agtttgccat cacttccagc gccacgcctt gcaggtaagc gtgcggtgtt
    65401 tcagcgaaca ggaacatcgc ttcgccaggg ttcaatttca ccacattcag caatagcggg
    65461 gagaacagac cgctgtcttc cgggtaaaat tcagaaatta aacgaatcgt ttgccacggt
    65521 tcaccctgct ggctatcgag ggccgatttt aaaatcgcca gcgcgcggga tttttcttca
    65581 ccctgcatat tcaacaggct ggcgaacagt tcgcttaaac gttcggcatc aggctgttgt
    65641 aaaaagtgag caatcgccgg atgtgcacct gcgaccggct ggagtaggga gacaatctcg
    65701 gaaaattcac gaaacgcgtt catcgcaagg aaaggcgtca gcgcaaaaac cagctccggc
    65761 ttgtggttag gatctttata gttacgctcg gcggcatcca tcgggatacc tgcggcattt
    65821 tctttggcaa aaccgatttc agaattgtgt ttgtttggat gaacctgaat ggagagtggc
    65881 tgtgctgcgc ataatacttt gaacaggaaa ggcagttcgc caaagcgttt ggcaacggcc
    65941 tctccgagca gagtcgattt atcactctca atcacatcac gcagtgaaac gatatctccg
    66001 gcggcattct gcactcgtga actgcttttc ggatgtgcgc ccatccacag ctcggccatc
    66061 ggctggctgg acggattttc cataccataa agttcagtca acgccgtttt gctgccccag
    66121 gcatagtttt gcactgagtt aatgagtttt tgcatgatca atccctgttt taatgtggaa
    66181 attaatccca ctattaaagc aagaatccta cgggaagtaa cctggagccg caaaaagtcg
    66241 tactagtctc agtttttgtt aaaaaagtgt gtaggatatt gttactcgct tttaacaggg
    66301 caacggaaca cccgcccaga gcataaccaa accaggcagt aagtgagaga acaatgtcaa
    66361 acaaaccctt tcattatcag gctccttttc cactcaaaaa agatgatact gagtattacc
    66421 tgctaaccag cgaacacgtt agcgtatctg aatttgaagg gcaggagatt ttgaaagtcg
    66481 cacccgaagc gttaactctg ttggcgcgcc aggcgtttca tgatgcgtcg ttcatgctgc
    66541 gtccggcgca ccaacaacag gtggccgaca ttctgcgtga cccggaggcc agcgaaaatg
    66601 ataaatatgt ggcgctgcaa ttcctgcgta actccgacat cgcggcgaaa ggcgttctgc
    66661 caacctgtca ggataccggc accgcgatta ttgttggtaa aaaagggcag cgtgtatgga
    66721 ccggtggtgg tgatgaagcg gcgctggcgc gcggtgtcta taacacttat atcgaagata
    66781 atctgcgcta ctcgcaaaac gcgccgctgg atatgtataa agaagtgaat accggcacca
    66841 atctgccagc gcagatcgat ctttatgccg ttgatggcga cgaatacaaa ttcctctgta
    66901 tcgccaaagg tggtggttcg gcaaacaaga cgtatctcta tcaggaaacc aaagcgttac
    66961 tgacgccggg gaaactgaaa aattacctgg ttgagaagat gcgcacgctg ggtacggcgg
    67021 cctgtcctcc gtatcatatt gcgttcgtta ttggtggaac ttctgcagaa acgaacctta
    67081 aaacggtgaa actggcttcc gcgaaatact atgatgaact gccaacggaa gggaatgagc
    67141 acggtcaggc gttccgcgat gtggaactgg aaaaagaatt gctgatcgaa gcgcaaaatc
    67201 ttggtctggg tgcgcagttt ggtggtaaat acttcgctca cgacatccgc gtgattcgcc
    67261 tgccacgtca cggcgcatcc tgcccggtcg gtatgggcgt ctcctgctct gctgaccgta
    67321 atatcaaagc gaagatcaac cgtcagggga tctggatcga aaaactggaa cataatccag
    67381 gcaaatatat cccggaagag ctgcgcaaag cgggagaagg cgaagcggtg cgcgttgacc
    67441 ttaaccgtcc gatgaaagag atcctcgcac agttgtcgca gtatcccgtt tctacacgct
    67501 tatcgcttaa cggcacgatt atcgtcggtc gtgatattgc tcacgccaaa ctgaaagagc
    67561 ggatggataa cggtgaaggg ctgccgcagt acatcaaaga tcatccgatt tactacgcgg
    67621 gtccggccaa aacgccggaa ggttatgcct ccggttctct tggcccaacg accgccggac
    67681 ggatggattc ttatgtcgat caactgcaag cgcagggcgg aagtatgatc atgctggcga
    67741 aaggcaaccg cagccagcag gtgacggatg cctgtaaaaa acacggcggc ttctaccttg
    67801 gcagtatcgg tggtccggcc gctgtattgg cgcagggaag tattaagagc ctggaatgtg
    67861 ttgaatatcc ggaactggga atggaagcca tctggaaaat tgaagtggaa gatttcccgg
    67921 cgtttatcct tgtggatgat aaaggaaatg acttcttcca gcagatacaa ctcacacaat
    67981 gcacccgctg tgtgaaataa acagagccgc ccttcggggc ggttttttta catggcacga
    68041 aagaccaaac atttgttatc aaatggtaaa taataagtga gctaaaagtt gcttaacgaa
    68101 agcaaaacag aaagaaaaaa ttaatcaggt gaggagcagg tcatgaatac agtacgcagc
    68161 gaaaaagatt cgatgggggc gattgatgtc ccggcagata agctgtgggg cgcacaaact
    68221 caacgctcgc tggagcattt ccgcatttcg acggagaaaa tgcccacctc actgattcat
    68281 gcgctggcgc taaccaagcg tgcagcggca aaagttaatg aagatttagg cttgttgtct
    68341 gaagagaaag cgagcgccat tcgtcaggcg gcggatgaag tactggcagg acagcatgac
    68401 gacgaattcc cgctggctat ctggcagacc ggctccggca cgcaaagtaa catgaacatg
    68461 aacgaagtgc tggctaaccg ggccagtgaa ttactcggcg gtgtgcgcgg gatggaacgt
    68521 aaagttcacc ctaacgacga cgtgaacaaa agccaaagtt ccaacgatgt ctttccgacg
    68581 gcgatgcacg ttgcggcgct gctggcgctg cgcaagcaac tcattcctca gcttaaaacc
    68641 ctgacacaga cactgaatga gaaatcccgt gcttttgccg atatcgtcaa aattggtcgt
    68701 actcacttgc aggatgccac gccgttaacg ctggggcagg agatttccgg ctggatagcg
    68761 atgctcgagc ataatctcaa acatatcgaa tacagcctgc ctcacgtagc ggaactggct
    68821 cttggcggta cagcggtggg tactggacta aatacccatc cggagtatgc gcgtcgcgta
    68881 gcagatgaac tggcagtcat tacctgtgca ccgtttgtta ccgcgccgaa caaatttgaa
    68941 gcgctggcga cctgtgatgc cctggttcag gcgcacggcg cgttgaaagg gttggctgcg
    69001 tcactgatga aaatcgccaa tgatgtccgc tggctggcct ctggcccgcg ctgcggaatt
    69061 ggtgaaatct caatcccgga aaatgagccg ggcagctcaa tcatgccggg gaaagtgaac
    69121 ccaacacagt gtgaggcatt aaccatgctc tgctgtcagg tgatggggaa cgacgtggcg
    69181 atcaacatgg ggggcgcttc cggtaacttt gaactgaacg tcttccgtcc aatggtgatc
    69241 cacaatttcc tgcaatcggt gcgcttgctg gcagatggca tggaaagttt taacaaacac
    69301 tgcgcagtgg gtattgaacc gaatcgtgag cgaatcaatc aattactcaa tgaatcgctg
    69361 atgctggtga ctgcgcttaa cacccacatt ggttatgaca aagccgccga gatcgccaaa
    69421 aaagcgcata aagaagggct gaccttaaaa gctgcggccc ttgcgctggg gtatcttagc
    69481 gaagccgagt ttgacagctg ggtacggcca gaacagatgg tcggcagtat gaaagccggg
    69541 cgttaatctg caacatacag gtgcagccgt ggaatgatca aacgaagcgg ctgcgcctga
    69601 ggtttataac ggtgctgcac attgtcggca tcgtaattta acaactcacc aacgtccggc
    69661 acgcccgcgc cattatcccg attaatcagt gcaatcagtg gtgtagggca ggcgtgttgg
    69721 acttgttttt gatctccttt gtaccagacg cgggcaatcg gctgcacctt caccggacgt
    69781 ttgattttta acttcgcgtt ctgtggcagg gcagcgatat cctgatactc tcgctccagt
    69841 tttctttgcc actcctcgcg cgtccacggt gcgacactgc gtggtgattt caggcttttt
    69901 tccagctgtg ccaggacttc atcacgatgt aaattcttaa tgatatgttt attagcccaa
    69961 ccaaagcgta aagtggcggg gtcgtgcaga acggtgagcg tgcggtaagc attaagggtg
    70021 atcagccccg gcaaatgacg atgcacccat tcaaaacgtg ccgcggtggg gagttctgat
    70081 tcaaccgtga cgatatgctc gaacgtggtc ttgagtttat tgatgtgctg aatatgactg
    70141 accaacgctg cttgcgaaag gttatcgacc tggtaacaca acacgccagg cagacggaca
    70201 gcggccttgc tgctgcgatt ttcggactgt tgttgaataa ataaatggcg gaaatgacgc
    70261 aacgccagcg actgcgcgtc gttgccgaga tgttgtttta cctcaatacg attaagcgga
    70321 ttatgctcat cctctttttt tacctccggc aaagagaaca cgcgggcaac caatagcttg
    70381 tgttgctcaa gatgagcggc aaatatagcc agctcttgtt ccatctggcg aaaggtagtg
    70441 ttgagtcggt ctacgagatc gtaacgcgcc ataatattaa ccactttagt tacaacatac
    70501 ttattttata gcacagtcgt ggtgacttga ccatcgtgcg cgtagtgtca ggcagaggta
    70561 aattgcggga tgttatgcca taacggccag ctaaacgaga agcgggcacc acccagttcg
    70621 ctggtgtcac aattaaccgt accgcccatt gccagtgcta tagagtggac aattgccagc
    70681 cccagcccgc agccgccggt tgagcgatcc cggctgggat cgaggcgaac aaaaggttca
    70741 aagatatgtt cgcggttttc tggggcaatc cctgggccat catcctcaac aattaatgtc
    70801 gctctattcc ccgacagtag caggctggtt tcaaccgttg aatggcagta ccgcagggcg
    70861 ttattgagca aattatccag cacgcgctcc attaagcgca tatccaacgc cgcataatgg
    70921 ccttgcacga gcgtttttat ccgtaccgtt ttatcgggcg ttactgcctg aatatctgcc
    70981 agatgcgttg acagccacaa cggcaggtct ggttcgctaa gatgaagctc gttttgtggg
    71041 cgatcgagtc gggcataagt cagcagctct tcaattaaag cttcaagttg actgatatca
    71101 cgattcaacg cctgggattc ggcggcgctc aggttatcgc tcatctccag tcgataacgc
    71161 aggcgcacta acggtgttcg cagttcgtga gcgataccgt caataagctg ttttttgctg
    71221 gcaattaagg cgttgatatt gtccgccatc tggttaaatg cgacgccaag tcgttcaaag
    71281 ctcgaaccct catcaaagtg gatacgttca ttgagatgcc catcgccaaa tcgttgcgcc
    71341 gctgcttcca gttttaacat atcctgccag tgcggacgca tccagataaa caccggaaag
    71401 gcgagggaaa tagcaataaa agcgatcagg gcgatatcca gcaatcgcat ctgatggaga
    71461 taataaagat aaggaacagg accaactgcc agcacgtagt ggctgcgcgg gatacgctgc
    71521 aaaaacgtgt actgatcgtc cagggcgaca atttcgccgc cacgcagtcg gtgcatggaa
    71581 atatcatcaa gatggtattt actcagtggc tcgacacgca gatcgaaaga gagatttaaa
    71641 tccatctctt tcagagtttt accccagtcg tgtgggggga tctcacgcaa ttcgctgcgc
    71701 atcagataca gcgaactgtt catcaaatca tccagcgact gtttgcccgc gcgttcggcg
    71761 gtaaatttgt acaccagccc aaccagcaga gacatcacaa ggaagcagac aaacaataac
    71821 aggtaaaact ggataaacag ttttttcatc gcttattccc atgcatgagg cgcaaaaaga
    71881 tagcctttgt tacgcacagt tttaatgcga taaggttctg cggcgttatc gagcagtttt
    71941 tttcttaacc gcgaaatagc cacgtccacg ctacgatcca gtccgtcata actgacgccg
    72001 cgtaaatttt tcagcaatgc atcgcggtcc atgatttgcc cggcatgggt agctaattcc
    72061 cacaataatt cgaaatcagc tgtcgagagc gagatttcag tgttagccag ggtgactacg
    72121 cggttgatgg gatcgatggt caacgtgccg aaatgcaggg ctttgtaggg agtcagagac
    72181 gtttcctgaa gacctttggt cagtgtggct tgctcattct gacgcaaatg caaacgtaaa
    72241 cgcgctagca aaacagcagg gggcgtcgtt ttgagaatat agtcgcaggc acccatttcc
    72301 agtgccagga tgtggttcat atcgctatcg agagaggtta gaagaacaat cggtccagac
    72361 cactttgcgc gtaaatcacg acaaatggtc atgccgtcct tgcctggtag catgatgtcg
    72421 agtaacacca aatccggatt ttctcgcaaa atggtttctt cggcctggtc gccgcgcggc
    72481 tctacggtaa cctgcatatc atgttttgcc aggtacgcgg caatcagtga accgacttcc
    72541 gcatcatctt ccacaaatac gatagtgttc ataacattca cggtagatat aaaaacgtca
    72601 caatacaccg cgctgttttt acttactatc aatcgtttct aaacaagttt tcatcaggta
    72661 ttctactgat gacttgttat ggaagtgtta aggtaaaaag atggggctgg taatcaaagc
    72721 cgcgctcggt gcgctggtag tgctgttgat tggtgtttta gcaaaaacga aaaattatta
    72781 tatcgccggg ctgattccac ttttcccgac ctttgcgctt atcgcgcatt atattgttgc
    72841 cagcgaacgc ggcattgaag ccttacgcgc aaccatcatt tttagtatgt ggtcgattat
    72901 tccctatttt gtttacctgg tgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc
    72961 cgcttttgtt ggttctgttg cgtgttgggg gataagcgca tgggtgttga ttatatgctg
    73021 gataaagctg cattaacgca gatgacgacc gccatcaagt gggaaactgc gtccggtgac
    73081 aaagcaactg gtaagtaagt aatcgaccag gtcgatcact tctttctcgc caggcgcggt
    73141 tttcatcagt gatttattca gcgcctgttg tcgatattcg gcatcatcat gttcattaaa
    73201 caggatcagc gatggcgcaa tagaattcac tttcacttcc ggtgccagct tgcgggcaaa
    73261 cgagcgggtc atattatcca gtgccgcttt gcttgcagca tacgcaatat gtttgtcgct
    73321 accgcgctcc accacataat cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc
    73381 acgcagtaat ctttccagcg catggttgag caggtatggg gtattaacgt ggatctgcat
    73441 catgcaagcc agtacgtcgg ccagtggcgc acccggtttt tccgccatcc acgcactggc
    73501 gttatgcaaa atagcacgca gaccatgggt gctttttagt acttcatcgg caaacgccat
    73561 cacaccgtcg ttggtcgaaa aatcagcctg aatacactgc gcacctgcat taatcagtcc
    73621 atcaatggct ggatagtgtg tccgatagct gacaatcacc ggttgctttt gattaatgaa
    73681 atgccatgcg agggcgaggc cgatgcgacg acctccgcca gtaattaata ttggcaaggg
    73741 ctgggtttta cccatcgtta tctcctttgc tatccaacga tgggatgaac gttatcccac
    73801 cagcatccag gtcgccggaa ctgaggcaat taacagcatg ccaatcagca ccatttcctg
    73861 acggttcagt acattatcat gggtatgcgt tttgcgcgcg tacaggaaaa ccagcagccc
    73921 cggtgcatac agaacaacgg ataacagcaa gtgcattgga ccagaggcgt acaataacca
    73981 taagccataa atgcaggcac cgacacctac cgctttatgc agtggacggg ttgcgatttt
    74041 cagcaagaac gcgccgacca ggaaataggg caccagaatc atttctgagg cgatggtcag
    74101 caacgtgttg taatcggaac cggtcagcca gatgagtacc agacaaattt gcacgcagat
    74161 gttggtcaac cacagtgagg cggatggcgc agcttgtgcg ttctggcgcg caaaaatgcg
    74221 cgggaatgct ttatgagtgg cggccaggaa cggtacttct gctgccatga tggtccagct
    74281 taagtacgcg ccgcaaacgg aaacgatcaa accggcagcg atgatgattt cgccccatgg
    74341 tcccatcatt tccaccatca gaccggccat tgacgggtta cgaatttcag ccagttcagg
    74401 acgtgccacc acacccagcg aaagcagcgt taccagcaag taaacgccca gagcggagag
    74461 aaccgccagc agtgtcgctt tgccaacatc acgtttatta cgcgcacgcg cagaaacaac
    74521 cacggcacct tccacaccaa tgaaaaccca cagggtgatc agcatggtgt ttttcacctg
    74581 ttcccaaacg ggtacgccaa gtgcaagtcc ggtgaagtcg agcttgaagg tatccagttt
    74641 gaacatcatc atcgccagca caacaaacag acccagcggc aacaattttg ccagtgtcgc
    74701 caccaggtta atgctggcag cggtttgcac cccgcgcagg atcaaaaagt gaacaatcca
    74761 caataaagcc gatgcaccga ctatcgattg ccaggtatta ccatcgccaa acaggcgcaa
    74821 ttccggcgtg tccgtaaaaa agcttaacgc ggaaaaaacg atcaccagat aggagacgtt
    74881 ggcgatgact gcgcacagcc agtatcccca tgcggaacaa aagccgatta gctcgccaaa
    74941 cccttcgcgg gcataggtaa agataccgcc gtcaagttcg ggacgaatgc gcgtgaggat
    75001 cagcatggca aaggccagca ataaaatgcc agcgccagta ataccccagc cgatgagcag
    75061 tgctgccggg ctggcaactg ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag
    75121 cattgagctt aataccagcg cggtgagtgc gctcagtccc agtttctttt ccatcgatat
    75181 ccagtgatta aaacgtgata agaagaataa atattagggc aaaacgcata aaaatggagt
    75241 ttgccactga agcgcgggat tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa
    75301 atttgtgact aaaaatgcga aaggcggcat caagccgcct tataggagta actgaatagt
    75361 tatttataca gatctgcgct gacggtcagg ttattaccac gttcctgcca ctggcgggtg
    75421 atgtggtaat acttagcacc tttcttcgcg gcacgtttcg caacctgata ggagacttcg
    75481 gtcatgttgc cgtagttgcc agagaatttg atgctgtcga acgggaccat catcgctgcg
    75541 gtcgctttgt tcagttcttc gactttagtg ccatccggga gcgtgacggt gtaacgcccg
    75601 ccttttgatg actgggtttc aaagaagcga ccgacttcag aacttggtga tgcggtagtc
    75661 gcaacacccg ggatctcaac tttcttcgcg gcttcgccac cggcagccag agctgcacgt
    75721 cctgcttcgg aatctgccgg gatcacatcc gggctctgga cgatacgttt cttagcatct
    75781 tttttataga tgaatgcagt aatacgctgg ttgccgccct ggttggcatc gatttgacga
    75841 acgatgtaga aagagtaggc acctttcgct tttgccgctt tggtgatggc atcattgact
    75901 tctggctggc tacgatagaa gccctggacg gtgaccgtgt caaacggttc aatcagaaca
    75961 gcctgatctt tcggcagttc gacaacaccg ttaattacgc gattacttgt ttcttctgct
    76021 ttttcagcat cggctttata gaggtcagcg accacacgcc agttaccgct gttaccaaaa
    76081 tcagaagtgt cgacaacata aaaagaggcg gcaccttctt tatctgcgcg acgagaaacg
    76141 gctttcaccg cttcgccaat agcattaaaa cgaccggtaa ccactacacg gtcaaaaggt
    76201 ttaaccgctg ccgcttgctc cggtgtcagt tctgttgctg cgttaacgga gaatgccgta
    76261 gcagaaagca gtgccgacgc caggagggtg ttcttaagct tcataaaaat aatccttcgc
    76321 cttgcgcaaa ccaggtactg gtattgttat taacgagaaa cgtggctgat tattgcattt
    76381 aaacggtgta actgtctgcg tcatttttca tatcacattc cttaagccaa ttttaatcct
    76441 gctcaaatga ccgtctatgc ttaaaaaaca gccgtatcag catcattact actgaagcaa
    76501 ctgaattgta taagttaatt taatgttaag tagtgattcg tgccggggcg atgtctcgtt
    76561 ttacccgacc gtcgaagaca attatcagtc tttatccggc gttctaaggt gtttatccca
    76621 ctatcacggc tgaatcgtta atattttgcg agttcacgcc gaaatactga tttttggcgc
    76681 tagatcacag gcataatttt cagtacgtta tagggcgttt gttactaatt tattttaacg
    76741 gagtaacatt tagctcgtac atgagcagct tgtgtggctc ctgacacagg caaaccatca
    76801 tcaataaaac cgatggaagg gaatatcatg cgaattggca taccaagaga acggttaacc
    76861 aatgaaaccc gtgttgcagc aacgccaaaa acagtggaac agctgctgaa actgggtttt
    76921 accgtcgcgg tagagagcgg cgcgggtcaa ctggcaagtt ttgacgataa agcgtttgtg
    76981 caagcgggcg ctgaaattgt agaagggaat agcgtctggc agtcagagat cattctgaag
    77041 gtcaatgcgc cgttagatga tgaaattgcg ttactgaatc ctgggacaac gctggtgagt
    77101 tttatctggc ctgcgcagaa tccggaatta atgcaaaaac ttgcggaacg taacgtgacc
    77161 gtgatggcga tggactctgt gccgcgtatc tcacgcgcac aatcgctgga cgcactaagc
    77221 tcgatggcga acatcgccgg ttatcgcgcc attgttgaag cggcacatga atttgggcgc
    77281 ttctttaccg ggcaaattac tgcggccggg aaagtgccac cggcaaaagt gatggtgatt
    77341 ggtgcgggtg ttgcaggtct ggccgccatt ggcgcagcaa acagtctcgg cgcgattgtg
    77401 cgtgcattcg acacccgccc ggaagtgaaa gaacaagttc aaagtatggg cgcggaattc
    77461 ctcgagctgg attttaaaga ggaagctggc agcggcgatg gctatgccaa agtgatgtcg
    77521 gacgcgttca tcaaagcgga aatggaactc tttgccgccc aggcaaaaga ggtcgatatc
    77581 attgtcacca ccgcgcttat tccaggcaaa ccagcgccga agctaattac ccgtgaaatg
    77641 gttgactcca tgaaggcggg cagtgtgatt gtcgacctgg cagcccaaaa cggcggcaac
    77701 tgtgaataca ccgtgccggg tgaaatcttc actacggaaa atggtgtcaa agtgattggt
    77761 tataccgatc ttccgggccg tctgccgacg caatcctcac agctttacgg cacaaacctc
    77821 gttaatctgc tgaaactgtt gtgcaaagag aaagacggca atatcactgt tgattttgat
    77881 gatgtggtga ttcgcggcgt gaccgtgatc cgtgcgggcg aaattacctg gccggcaccg
    77941 ccgattcagg tatcagctca gccgcaggcg gcacaaaaag cggcaccgga agtgaaaact
    78001 gaggaaaaat gtacctgctc accgtggcgt aaatacgcgt tgatggcgct ggcaatcatt
    78061 ctttttggct ggatggcaag cgttgcgccg aaagaattcc ttgggcactt caccgttttc
    78121 gcgctggcct gcgttgtcgg ttattacgtg gtgtggaatg tatcgcacgc gctgcataca
    78181 ccgttgatgt cggtcaccaa cgcgatttca gggattattg ttgtcggagc actgttgcag
    78241 attggccagg gcggctgggt tagcttcctt agttttatcg cggtgcttat agccagcatt
    78301 aatattttcg gtggcttcac cgtgactcag cgcatgctga aaatgttccg caaaaattaa
    78361 ggggtaacat atgtctggag gattagttac agctgcatac attgttgccg cgatcctgtt
    78421 tatcttcagt ctggccggtc tttcgaaaca tgaaacgtct cgccagggta acaacttcgg
    78481 tatcgccggg atggcgattg cgttaatcgc aaccattttt ggaccggata cgggtaatgt
    78541 tggctggatc ttgctggcga tggtcattgg tggggcaatt ggtatccgtc tggcgaagaa
    78601 agttgaaatg accgaaatgc cagaactggt ggcgatcctg catagcttcg tgggtctggc
    78661 ggcagtgctg gttggcttta acagctatct gcatcatgac gcgggaatgg caccgattct
    78721 ggtcaatatt cacctgacgg aagtgttcct cggtatcttc atcggggcgg taacgttcac
    78781 gggttcggtg gtggcgttcg gcaaactgtg tggcaagatt tcgtctaaac cattgatgct
    78841 gccaaaccgt cacaaaatga acctggcggc tctggtcgtt tccttcctgc tgctgattgt
    78901 atttgttcgc acggacagcg tcggcctgca agtgctggca ttgctgataa tgaccgcaat
    78961 tgcgctggta ttcggctggc atttagtcgc ctccatcggt ggtgcagata tgccagtggt
    79021 ggtgtcgatg ctgaactcgt actccggctg ggcggctgcg gctgcgggct ttatgctcag
    79081 caacgacctg ctgattgtga ccggtgcgct ggtcggttct tcgggggcta tcctttctta
    79141 cattatgtgt aaggcgatga accgttcctt tatcagcgtt attgcgggtg gtttcggcac
    79201 cgacggctct tctactggcg atgatcagga agtgggtgag caccgcgaaa tcaccgcaga
    79261 agagacagcg gaactgctga aaaactccca ttcagtgatc attactccgg ggtacggcat
    79321 ggcagtcgcg caggcgcaat atcctgtcgc tgaaattact gagaaattgc gcgctcgtgg
    79381 tattaatgtg cgtttcggta tccacccggt cgcggggcgt ttgcctggac atatgaacgt
    79441 attgctggct gaagcaaaag taccgtatga catcgtgctg gaaatggacg agatcaatga
    79501 tgactttgct gataccgata ccgtactggt gattggtgct aacgatacgg ttaacccggc
    79561 ggcgcaggat gatccgaaga gtccgattgc tggtatgcct gtgctggaag tgtggaaagc
    79621 gcagaacgtg attgtcttta aacgttcgat gaacactggc tatgctggtg tgcaaaaccc
    79681 gctgttcttc aaggaaaaca cccacatgct gtttggtgac gccaaagcca gcgtggatgc
    79741 aatcctgaaa gctctgtaac cctgacggcc tctgctgagg ccgtcactct ttattgagat
    79801 cgcttaacag aacggcgatg ctttgacctc ccgctgtttg ttcaagcgca attttgacaa
    79861 taattgtcaa cggcacggaa agcagcatac ccaccggtcc taacaaccat ccccaaaaaa
    79921 tcaacgacaa aaataccacc aatgtggaaa gccccagccc acgccccatg atacgcggct
    79981 ccagaatatt gccgaagacc agattaatca gcagatatcc cgccagcacc agcaacgctt
    80041 cgtagaagcc attaaacacc agtacctgag cgataggggg gattgccgcg aggactgaac
    80101 caatattcgg gatgtaatta agcgcaaagg ccagcaatcc ccagacaaaa gcgaagcgaa
    80161 catcgagtgc ggcgagcatc gcccaggcga ccaggccggt gatgatgctg atggctgttt
    80221 tcagcaccag ataatgagaa acactgtcaa tcgcacgttg aatcgccgcc atcccttcaa
    80281 ccggacgcgc catcatttgc tgaaattttc cgggcaattg tggcacttcg agcagcataa
    80341 acagcaccgt cagcagcaat aaaaatattg atgacatggc attagataac tgcgtcaata
    80401 agttggtgag caacgtcatc gccgcgttcg gatcaatata atgcgccagc tggtcaactg
    80461 agacgtcaat ccctacgcgt tgcaacaacg gttcaagcgc ttgcagcggc gtcataatag
    80521 agttgcgata ttgcggtaac gtccgcgtca actcgttgag cgcggaaccc agataagcta
    80581 atagcaacac catcgccatc acgatgatgg tcatcaaaat cgacaccgcc agtacacgcg
    80641 gcacacgcca gcggaccatg tgttgcacca gcgggttaag aataacagca ataaataatg
    80701 cgagaataaa cggcacgatg atctcggcgg caaaacggat accgcagaga ataatgacca
    80761 gcattcccaa cataatgacg atttttaggc cattgagcgt gatgatcggc tttgccatgc
    80821 ttgtccccgt tttttcttcc tttgctaata ataatgtctt tttcgctccg tcatcataac
    80881 gaaaatcaaa ccgctcaggt aaagaagtga aaatattttg agttaattct taagctatga
    80941 tacaaatcag gcgtgttcaa ctaccgagga caattatcat ccgcgatgac gagaagcaac
    81001 actgcggata attgtaatat tatggacaat ttgttcagga cgttattttc tacatttacc
    81061 catcttcgta cctcatccac tatcttgctc gtaggtgagc agcactggcg caacgcgctt
    81121 tagtcctttc ttctgccaaa gcttgcgcta tgcgcggggc ttgaccactt tactttggtt
    81181 tcgctgaatt aagcgaaaat taaaataatt c
//
LOCUS       DAZFZC010000008        80996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFZC010000008 DAZFZC010000000
VERSION     DAZFZC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.008"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(134..991)
                     /gene="murI"
                     /locus_tag="ACHKXP_000652"
     CDS             complement(134..991)
                     /gene="murI"
                     /locus_tag="ACHKXP_000652"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO7645721.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTTEAEQLLPVLQRYGFG
                     TLEKLAVLG"
     gene            complement(936..2819)
                     /gene="btuB"
                     /locus_tag="ACHKXP_000653"
     CDS             complement(936..2819)
                     /gene="btuB"
                     /locus_tag="ACHKXP_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO7645722.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKNINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     regulatory      complement(2884..3074)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3188..4288
                     /gene="trmA"
                     /locus_tag="ACHKXP_000654"
     CDS             3188..4288
                     /gene="trmA"
                     /locus_tag="ACHKXP_000654"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO7645723.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4328..4687)
                     /gene="yijD"
                     /locus_tag="ACHKXP_000655"
     CDS             complement(4328..4687)
                     /gene="yijD"
                     /locus_tag="ACHKXP_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO7645724.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4687..5337)
                     /gene="fabR"
                     /locus_tag="ACHKXP_000656"
     CDS             complement(4687..5337)
                     /gene="fabR"
                     /locus_tag="ACHKXP_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO7645725.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5668..7068
                     /gene="sthA"
                     /locus_tag="ACHKXP_000657"
     CDS             5668..7068
                     /gene="sthA"
                     /locus_tag="ACHKXP_000657"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO7645726.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7051..7968)
                     /gene="oxyR"
                     /locus_tag="ACHKXP_000658"
     CDS             complement(7051..7968)
                     /gene="oxyR"
                     /locus_tag="ACHKXP_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO7645727.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8235..9608)
                     /gene="argH"
                     /locus_tag="ACHKXP_000659"
     CDS             complement(8235..9608)
                     /gene="argH"
                     /locus_tag="ACHKXP_000659"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO7645728.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9669..10445)
                     /gene="argB"
                     /locus_tag="ACHKXP_000660"
     CDS             complement(9669..10445)
                     /gene="argB"
                     /locus_tag="ACHKXP_000660"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO7645729.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10453..11457)
                     /gene="argC"
                     /locus_tag="ACHKXP_000661"
     CDS             complement(10453..11457)
                     /gene="argC"
                     /locus_tag="ACHKXP_000661"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO7645730.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11611..12762
                     /gene="argE"
                     /locus_tag="ACHKXP_000662"
     CDS             11611..12762
                     /gene="argE"
                     /locus_tag="ACHKXP_000662"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO7645731.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            13237..15888
                     /gene="ppc"
                     /locus_tag="ACHKXP_000663"
     CDS             13237..15888
                     /gene="ppc"
                     /locus_tag="ACHKXP_000663"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO7645732.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            16070..17803
                     /gene="eptC"
                     /locus_tag="ACHKXP_000664"
     CDS             16070..17803
                     /gene="eptC"
                     /locus_tag="ACHKXP_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7645733.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            18018..18869
                     /gene="yijO"
                     /locus_tag="ACHKXP_000665"
     CDS             18018..18869
                     /gene="yijO"
                     /locus_tag="ACHKXP_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7645734.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18856..19197)
                     /gene="frwD"
                     /locus_tag="ACHKXP_000666"
     CDS             complement(18856..19197)
                     /gene="frwD"
                     /locus_tag="ACHKXP_000666"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7645735.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGTERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19199..20077)
                     /gene="pflC"
                     /locus_tag="ACHKXP_000667"
     CDS             complement(19199..20077)
                     /gene="pflC"
                     /locus_tag="ACHKXP_000667"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO7645736.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(20043..22340)
                     /gene="pflD"
                     /locus_tag="ACHKXP_000668"
     CDS             complement(20043..22340)
                     /gene="pflD"
                     /locus_tag="ACHKXP_000668"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7645737.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRFGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22391..22711)
                     /gene="frwB"
                     /locus_tag="ACHKXP_000669"
     CDS             complement(22391..22711)
                     /gene="frwB"
                     /locus_tag="ACHKXP_000669"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7645738.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22726..23805)
                     /gene="frwC"
                     /locus_tag="ACHKXP_000670"
     CDS             complement(22726..23805)
                     /gene="frwC"
                     /locus_tag="ACHKXP_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO7645739.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24114..26615
                     /gene="ptsP"
                     /locus_tag="ACHKXP_000671"
     CDS             24114..26615
                     /gene="ptsP"
                     /locus_tag="ACHKXP_000671"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7645740.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQAAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQHVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26627..27289
                     /gene="fsa"
                     /locus_tag="ACHKXP_000672"
     CDS             26627..27289
                     /gene="fsa"
                     /locus_tag="ACHKXP_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7645741.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGIT
                     HL"
     gene            27300..28403
                     /gene="gldA"
                     /locus_tag="ACHKXP_000673"
     CDS             27300..28403
                     /gene="gldA"
                     /locus_tag="ACHKXP_000673"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO7645742.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28678..29295
                     /gene="yijF"
                     /locus_tag="ACHKXP_000674"
     CDS             28678..29295
                     /gene="yijF"
                     /locus_tag="ACHKXP_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO7645743.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFNWRMVGHYRYFVK"
     gene            complement(29322..30227)
                     /gene="yijE"
                     /locus_tag="ACHKXP_000675"
     CDS             complement(29322..30227)
                     /gene="yijE"
                     /locus_tag="ACHKXP_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO7645744.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(30320..32500)
                     /gene="katG"
                     /locus_tag="ACHKXP_000676"
     CDS             complement(30320..32500)
                     /gene="katG"
                     /locus_tag="ACHKXP_000676"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO7645745.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32829..33719)
                     /gene="metF"
                     /locus_tag="ACHKXP_000677"
     CDS             complement(32829..33719)
                     /gene="metF"
                     /locus_tag="ACHKXP_000677"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO7645746.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            33795..33929
                     /locus_tag="ACHKXP_000678"
     CDS             33795..33929
                     /locus_tag="ACHKXP_000678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645747.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(34068..36500)
                     /gene="metL"
                     /locus_tag="ACHKXP_000679"
     CDS             complement(34068..36500)
                     /gene="metL"
                     /locus_tag="ACHKXP_000679"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO7645748.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAIEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36503..37663)
                     /gene="metB"
                     /locus_tag="ACHKXP_000680"
     CDS             complement(36503..37663)
                     /gene="metB"
                     /locus_tag="ACHKXP_000680"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO7645749.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37940..38257
                     /gene="metJ"
                     /locus_tag="ACHKXP_000681"
     CDS             37940..38257
                     /gene="metJ"
                     /locus_tag="ACHKXP_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO7645750.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38441..39049
                     /gene="yiiX"
                     /locus_tag="ACHKXP_000682"
     CDS             38441..39049
                     /gene="yiiX"
                     /locus_tag="ACHKXP_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO7645751.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     TDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(39110..39322)
                     /gene="rpmE"
                     /locus_tag="ACHKXP_000683"
     CDS             complement(39110..39322)
                     /gene="rpmE"
                     /locus_tag="ACHKXP_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7645752.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            39525..41723
                     /gene="priA"
                     /locus_tag="ACHKXP_000684"
     CDS             39525..41723
                     /gene="priA"
                     /locus_tag="ACHKXP_000684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO7645753.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQHHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            41879..42904
                     /gene="cytR"
                     /locus_tag="ACHKXP_000685"
     CDS             41879..42904
                     /gene="cytR"
                     /locus_tag="ACHKXP_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO7645754.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            42996..43955
                     /gene="ftsN"
                     /locus_tag="ACHKXP_000686"
     CDS             42996..43955
                     /gene="ftsN"
                     /locus_tag="ACHKXP_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO7645755.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            44048..44578
                     /gene="hslV"
                     /locus_tag="ACHKXP_000687"
     CDS             44048..44578
                     /gene="hslV"
                     /locus_tag="ACHKXP_000687"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO7645756.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            44588..45919
                     /gene="hslU"
                     /locus_tag="ACHKXP_000688"
     CDS             44588..45919
                     /gene="hslU"
                     /locus_tag="ACHKXP_000688"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO7645757.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            45986..46912
                     /gene="menA"
                     /locus_tag="ACHKXP_000689"
     CDS             45986..46912
                     /gene="menA"
                     /locus_tag="ACHKXP_000689"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO7645758.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            47005..47490
                     /gene="rraA"
                     /locus_tag="ACHKXP_000690"
     CDS             47005..47490
                     /gene="rraA"
                     /locus_tag="ACHKXP_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO7645759.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(47575..47820)
                     /gene="zapB"
                     /locus_tag="ACHKXP_000691"
     CDS             complement(47575..47820)
                     /gene="zapB"
                     /locus_tag="ACHKXP_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO7645760.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            48245..49090
                     /gene="glpF"
                     /locus_tag="ACHKXP_000692"
     CDS             48245..49090
                     /gene="glpF"
                     /locus_tag="ACHKXP_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO7645761.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            49113..50621
                     /gene="glpK"
                     /locus_tag="ACHKXP_000693"
     CDS             49113..50621
                     /gene="glpK"
                     /locus_tag="ACHKXP_000693"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO7645762.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGSVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            50756..51766
                     /gene="glpX"
                     /locus_tag="ACHKXP_000694"
     CDS             50756..51766
                     /gene="glpX"
                     /locus_tag="ACHKXP_000694"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7645763.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLQLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVIGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            51863..52609
                     /gene="fpr"
                     /locus_tag="ACHKXP_000695"
     CDS             51863..52609
                     /gene="fpr"
                     /locus_tag="ACHKXP_000695"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO7645764.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPHCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESTIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(52614..53042)
                     /gene="uspD"
                     /locus_tag="ACHKXP_000696"
     CDS             complement(52614..53042)
                     /gene="uspD"
                     /locus_tag="ACHKXP_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO7645765.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(53069..53368)
                     /gene="yiiS"
                     /locus_tag="ACHKXP_000697"
     CDS             complement(53069..53368)
                     /gene="yiiS"
                     /locus_tag="ACHKXP_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO7645766.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(53580..54020)
                     /gene="yiiR"
                     /locus_tag="ACHKXP_000698"
     CDS             complement(53580..54020)
                     /gene="yiiR"
                     /locus_tag="ACHKXP_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7645767.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWLAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            54121..54720
                     /gene="yiiQ"
                     /locus_tag="ACHKXP_000699"
     CDS             54121..54720
                     /gene="yiiQ"
                     /locus_tag="ACHKXP_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO7645768.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            54828..55595
                     /gene="tpiA"
                     /locus_tag="ACHKXP_000700"
     CDS             54828..55595
                     /gene="tpiA"
                     /locus_tag="ACHKXP_000700"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO7645769.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(55650..56405)
                     /gene="cdh"
                     /locus_tag="ACHKXP_000701"
     CDS             complement(55650..56405)
                     /gene="cdh"
                     /locus_tag="ACHKXP_000701"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO7645770.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(56512..57501)
                     /gene="sbp"
                     /locus_tag="ACHKXP_000702"
     CDS             complement(56512..57501)
                     /gene="sbp"
                     /locus_tag="ACHKXP_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO7645771.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYINVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(57821..58783)
                     /gene="pfkA"
                     /locus_tag="ACHKXP_000703"
     CDS             complement(57821..58783)
                     /gene="pfkA"
                     /locus_tag="ACHKXP_000703"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO7645772.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(58964..59866)
                     /gene="fieF"
                     /locus_tag="ACHKXP_000704"
     CDS             complement(58964..59866)
                     /gene="fieF"
                     /locus_tag="ACHKXP_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO7645773.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(60015..60515)
                     /gene="cpxP"
                     /locus_tag="ACHKXP_000705"
     CDS             complement(60015..60515)
                     /gene="cpxP"
                     /locus_tag="ACHKXP_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO7645774.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            60665..61363
                     /gene="cpxR"
                     /locus_tag="ACHKXP_000706"
     CDS             60665..61363
                     /gene="cpxR"
                     /locus_tag="ACHKXP_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO7645775.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            61360..62733
                     /gene="cpxA"
                     /locus_tag="ACHKXP_000707"
     CDS             61360..62733
                     /gene="cpxA"
                     /locus_tag="ACHKXP_000707"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO7645776.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWGEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(62839..63513)
                     /gene="yiiM"
                     /locus_tag="ACHKXP_000708"
     CDS             complement(62839..63513)
                     /gene="yiiM"
                     /locus_tag="ACHKXP_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO7645777.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSDKIEDFS
                     RRLWGK"
     gene            complement(63662..64645)
                     /gene="kdgT"
                     /locus_tag="ACHKXP_000709"
     CDS             complement(63662..64645)
                     /gene="kdgT"
                     /locus_tag="ACHKXP_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO7645778.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(64905..65525)
                     /gene="sodA"
                     /locus_tag="ACHKXP_000710"
     CDS             complement(64905..65525)
                     /gene="sodA"
                     /locus_tag="ACHKXP_000710"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO7645779.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIMGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            65810..66844
                     /gene="rhaT"
                     /locus_tag="ACHKXP_000711"
     CDS             65810..66844
                     /gene="rhaT"
                     /locus_tag="ACHKXP_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO7645780.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(66841..67689)
                     /gene="rhaR"
                     /locus_tag="ACHKXP_000712"
     CDS             complement(66841..67689)
                     /gene="rhaR"
                     /locus_tag="ACHKXP_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO7645781.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLEWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(67763..68599)
                     /gene="rhaS"
                     /locus_tag="ACHKXP_000713"
     CDS             complement(67763..68599)
                     /gene="rhaS"
                     /locus_tag="ACHKXP_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO7645782.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            68887..70356
                     /gene="rhaB"
                     /locus_tag="ACHKXP_000714"
     CDS             68887..70356
                     /gene="rhaB"
                     /locus_tag="ACHKXP_000714"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO7645783.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFENQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            70353..71612
                     /gene="rhaA"
                     /locus_tag="ACHKXP_000715"
     CDS             70353..71612
                     /gene="rhaA"
                     /locus_tag="ACHKXP_000715"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO7645784.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSRRG"
     gene            72063..72887
                     /gene="rhaD"
                     /locus_tag="ACHKXP_000716"
     CDS             72063..72887
                     /gene="rhaD"
                     /locus_tag="ACHKXP_000716"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO7645785.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            72897..73211
                     /gene="rhaM"
                     /locus_tag="ACHKXP_000717"
     CDS             72897..73211
                     /gene="rhaM"
                     /locus_tag="ACHKXP_000717"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO7645786.1"
                     /translation="MIRKAFIMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(73252..74646)
                     /locus_tag="ACHKXP_000718"
     CDS             complement(73252..74646)
                     /locus_tag="ACHKXP_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO7645787.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            75123..75569
                     /gene="frvA"
                     /locus_tag="ACHKXP_000719"
     CDS             75123..75569
                     /gene="frvA"
                     /locus_tag="ACHKXP_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO7645788.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            75580..77031
                     /gene="frvB"
                     /locus_tag="ACHKXP_000720"
     CDS             75580..77031
                     /gene="frvB"
                     /locus_tag="ACHKXP_000720"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO7645789.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            77021..78091
                     /gene="frvX"
                     /locus_tag="ACHKXP_000721"
     CDS             77021..78091
                     /gene="frvX"
                     /locus_tag="ACHKXP_000721"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7645790.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYHALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            78091..79839
                     /gene="frvR"
                     /locus_tag="ACHKXP_000722"
     CDS             78091..79839
                     /gene="frvR"
                     /locus_tag="ACHKXP_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO7645791.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQVISADNVQGLLQRVPGIASLNIIDAQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(79889..80887)
                     /gene="yiiG"
                     /locus_tag="ACHKXP_000723"
     CDS             complement(79889..80887)
                     /gene="yiiG"
                     /locus_tag="ACHKXP_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO7645792.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQY
                     QLEAKKYVRRVRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREYNEMVDDYNSLR"
BASE COUNT        19820 a        21654 c        20540 g        18982 t
ORIGIN      
        1 tcaggagaac cccgctgacc cggcggcgtg tttgccgttg ttccgtgtca gtggtggcgc
       61 attataggga gttattccgg cctgacaaga ggaaatttaa aataattttc tgaccgcgca
      121 acattcaacc aaatcaacct aaaactgcca gtttttcgag cgttccgaag ccgtaacgct
      181 gtaaaacggg caataattgt tcagcttctg tcgtcattgc catacaaaag gcaatattcg
      241 catcggcaga ttttgcatcc ggagcttcat gttctaacaa ccaggccgtt cggcgagcaa
      301 tcgctgcgcc agaatccacc agccgggttc cctctggcag cacttgtaac agttcttctt
      361 gtagtagagg gaaatgggtg caacccaata caacggtatc tggcggctct ttcattctta
      421 accacggacg taagatacgt tttagtgcat ccagagaaac atcttcgcca tgtagcttcg
      481 cttcagccaa ctcaaccatc tctgccgagc ccagcatttc tatctggcat tcattagcga
      541 aacgcgcgat cagctcatga gtataagaac gtttaactgt tccgcgggtt gccagtaatc
      601 cgacaatgcc atttgccgtc agacgtgcag caggtttaat cgccggcacg acaccaacaa
      661 ccgggaagtc gaacttttcg cgtaatgcag gaagtgaaac ggtactggcg gtgttgcaag
      721 cgaccacagc cagcgcaagg ggataacgtt cttgcaccgc ggtgacaatt gccaccactc
      781 gctcaacaat aaacgcttcg cttttttcgc catacgggaa agcgacgtta tcgaaagcat
      841 aaatgtaatg gagatccggt aagagatgcc ggatctcgtc atagaccgac aacccaccga
      901 cgccggagtc aaacaccagc acggtgggac gtggttcaga aggtgtagct gccagacaag
      961 gtgtattccc gtcctgcagt ttggtagcca tagactgtct cataatcttt gtcgaacagg
     1021 ttggctattt taccacgaac tgtcagatga gaggtgaccg gatatgcaac cgcaagatcc
     1081 cacaagctca caccgcctaa tttaactgta tgtgatgagc cagaggtctc gtcataagcg
     1141 ttgtcgtatc gttgacctat atattggtaa gaaacgtccc attcgaaacc ataagcttgt
     1201 ccagtcaact cgtacttaac ctgctgtttt gcccgacggt acagcacttt atctgtttca
     1261 tcatcgcgcg ggtcaacata ttgtaacgtc aagcgatgtt caacaggacc ggttgtaata
     1321 tttcccgtcc attccagccc tttaatcgtg gctgatttca cattaatgta ctggttgaca
     1381 ttatttatgt ttttataatc aatcaggttc tgaatttcat aacgatatcc tgagagccgc
     1441 caatccagag tcccagttac cccctcaatt ccaatctccc actgtttaga ttcttctggc
     1501 ttcaaatttg ggttggctgc aataccagga ccataaattg aagcaaagcg agcagccccg
     1561 tattgctgtc caagagaagg cgaaagaaaa ctggtaccgt aagaaagtgt tgcctggtag
     1621 ccatcaataa actcccagcc agcagctgtc tgccaggtac catgccagcc aaactgttca
     1681 tcatgatctt ctcggccaga agcttccaaa gtcacattgg aaatctgttg ctgtccagtc
     1741 aggtataacc cggtggtatc gcgttcgtaa tcatctgatt ttgtaatgct gctggacgtc
     1801 agtttttcct gtttccaatc gatcccgccg ctaattgcac catgccctac ctcaatgcta
     1861 ttaccccact gaatataacg ctgttccatg tcatccaggg tggcatctcc cgcataacga
     1921 ccaagattat ttgtgtagtt ataattcttg agtcgttgat agttagcaat taactgagac
     1981 gaatagatgc cggagttata gcgtaggcca gtatcccagg actggttgta attctgagct
     2041 tcttcgttgc cagtagcaga accatacatc gagccctggt cgtaatcatt attagctgtg
     2101 tagctgtaac cacggaagaa ccccgagaag ttatcgttaa acttatgctg cagccccccc
     2161 cagaaaagtt tgttgcgata tccatcgcga tcgctatcac cactgtatgg tgagccaggc
     2221 tgaatgttaa aacctttggt cgtctggtaa gcacctgctg ctgttaccat cgtatcgcca
     2281 aaacgtttat ttattacccc atcataggct tgataaccat ttgtacccat tcctgcgttg
     2341 atctgagctt tctcatcatt actcattgta ataatgttca ccacacctcc gatggcacca
     2401 gaaccataaa cggcagaacg cggcccacga atatattcaa cacgctgtac aagtgatacc
     2461 gggaactggc taatatcaat tgcattgtcg ataccggcac gagccatcgg cactccgtca
     2521 atcaacacta aaacatggcg cgattcagtg ccacgaacat aaagagaaga actcgtcccc
     2581 attccaccag tttgagagat atttacaccg gggagatgac tcataacatc gttgaggtct
     2641 ttcgactgcc agcgttgaat atcctcacgg gtaacaatat cagtaggtgc aaggacagta
     2701 tttactggct gctgaaaacg gttagcagta acgacgagag tatccgggct ggtatcctgt
     2761 gcccaagcgg aaaaggctgt gacagaacac gccgtcagca gcgaagcttt tttaatcatt
     2821 gtaaagcatc cacaatagaa gaaggatgcc gcaggtttca tcaatattac gcgatgatga
     2881 gaaccagatg cgacgttggc cggcaggtct tcgggcttgg aggggtatct aagatactaa
     2941 gagatgatga cttcccaccg aatggcagtg tccacataac gcaatcatcg cacctttcct
     3001 taccgctgcg cgtcagctcc agattcgcac tggattccct attaactcac aggaccggca
     3061 agtggatgct acaggttgta acaagttact gtccagacgt agctcacaaa taggaattca
     3121 tcaagatctg gacatctgat gagcaatccc tacaatcgcc gcgtacttta atttttcagg
     3181 atacatcatg acccccgaac accttccaac agaacagtat gaagcgcagt tagccgaaaa
     3241 agtggtacgt ttgcaaagta tgatggcacc gttttctgac ctggttccgg aagtgtttcg
     3301 ctcgccggtc agtcattacc ggatgcgcgc ggagttccgc atctggcacg atggcgatga
     3361 cctgtatcac atcattttcg atcaacaaac caaaagccgc atccgcgtgg atagcttccc
     3421 cgccgccagt gaacttatca accagttgat gacggcgatg attgcgggtg tgcgtaataa
     3481 tcccgttctg cgccacaagt tgttccagat tgattacctc actacgctga gtaatcaggc
     3541 ggtggtttcc ctgctatacc ataagaagct ggatgatgag tggcgtcagg aagcggaggc
     3601 cctgcgcgat gcactgcgcg cgcagaatct gaatgtgcat ctgattggtc gggcaacgaa
     3661 aaccaaaatc gagctggatc aggattacat cgatgaacgt ctgccggtcg cagggaaaga
     3721 gatgatctac cgtcaggtag aaaacagctt tacccagccg aacgcggcga tgaatattca
     3781 gatgctggaa tgggcgctgg acgtaaccaa aggctcaaaa ggcgatttac tggagctgta
     3841 ctgcggcaac ggtaactttt cattagcgct ggcgcgtaat tttgatcggg tattagccac
     3901 cgaaatcgct aagccgtcgg ttgctgctgc gcaatacaac atcgcagcta accatattga
     3961 taacgtacaa attattcgta tggcggcaga agaatttact caggcgatga atggtgtgcg
     4021 cgagtttaac cgcctgcaag ggatcgactt aaagagttat cagtgcgaaa ccatttttgt
     4081 cgacccaccg cgcagcggtc tggacagtga aaccgagaaa atggtgcagg cgtatccgcg
     4141 tattttgtac atctcctgta acccggaaac gttatgcaag aatctggaaa cattaagcca
     4201 gacgcacaag gtcgaacgtc tggctctgtt tgatcagttc ccctacacgc accatatgga
     4261 gtgcggcgta ttactgaccg cgaagtaaaa cccatgccgg atgcgccagc atccggcata
     4321 ataccgatta ctcagcaact tcctgcttac ggttacgcat cttcgcgcca atccagaaca
     4381 ccataatgac ggagagtact gccgggaaga agttagagcc gatatccgga tattccgcac
     4441 gaactaccgt gctgtacagc agtacgccga gaataaaaca ggcagctgcc agacccggca
     4501 agcctaccgg catagtgcga ttaagataac gttgatgcag gcagtaaacc gtcagcacca
     4561 gggaaataat cgggaataca gaaaatggca caatggagct aaacagcgct gcgaaagtac
     4621 cattaatcga taagccagca actaacgcca gcagcagcgt acctctatct tgatttgctt
     4681 gtttcattac tcgtccttca catttcccgg aataattgcg gttttctctt gttcacggcg
     4741 ataccagtaa taagcccctt tcgaaatcat tcgcagttgc agtaccagtc gctcttctaa
     4801 ttgccgacgt tgttcgacgc cgacgtccaa cgcctcggca cccgcactga agacaattgt
     4861 caccattgct tcggcttgcg cttcagtaaa cgcacgcggc atatggtttt cgagttccag
     4921 atagtccgca agttccgcaa tgaagtgctg aatttcacgc gcaacggcgg cacgaaacgc
     4981 agcggaggtg ccggagcgtt cccgcaataa taaccggaag gcgttaggat tattaccgat
     5041 gaactccata aatgtggaga ccgaggtgcg gatcacactc ccgcctttgg cgatacgctg
     5101 acgcgcctgg cgcatgagtt ggcgtagcat taaaccgctc tcatcaacca tggtcagacc
     5161 cagttcgtct acgtcgcgga aatgccgata aaaagaggtg ggagcaatgc ccgcttcacg
     5221 cgccacttca cgcaaactca ggctggcgaa gctgcgttca gcacttaatt ggctaaatgc
     5281 ggcttccacc agcgaacggc gggttttttc tttttgttgc gctcttacgc ccatcacgat
     5341 gtctgaatcc ttgccaaaag tactgctggc actataccag agaatgaaca tactggattg
     5401 ctccagtatt gtgaatgaac ggtaacgcaa taaaacatat ttactgcaat tctttggccg
     5461 gttcttttac gtacagcgga aacctgccgc ttaaacggag agtatcgtcg ataaaaatcc
     5521 aataaaacgt cagggcaaaa gtaagaaaca gacaaagcaa aggccgctca ggatatagcc
     5581 agataaatga cggggatcaa ttggcttacc cgcgataaaa tgttaccatt ctgttgcttt
     5641 tatgtataag aacaggtaag ccctaccatg ccacattcct acgattacga tgccatagta
     5701 ataggttccg gccccggcgg cgaaggcgct gcaatgggcc tggttaagca aggtgcgcgc
     5761 gtcgcagtta tcgagcgtta tcaaaatgtt ggcggcggtt gcacccactg gggcaccatc
     5821 ccgtcgaaag ctctccgtca cgccgtcagc cgcattatag aattcaatca aaacccactt
     5881 tacagcgacc attcccgact gctccgctct tcttttgccg atatccttaa ccatgccgat
     5941 aacgtgatta atcaacaaac gcgcatgcgt cagggatttt acgaacgtaa tcactgtgaa
     6001 atattgcagg gaaacgctcg ctttgttgac gagcatacgt tggcgctgga ttgcccggac
     6061 ggcagcgttg aaacactaac cgctgaaaaa tttgttattg cctgcggctc tcgtccatat
     6121 catccaacag atgttgattt cacccatcca cgcatttacg acagcgactc aattctcagc
     6181 atgcaccacg aaccgcgcca tgtacttatc tatggtgctg gagtgatcgg ctgtgaatat
     6241 gcgtcgatct tccgcggtat ggatgtaaaa gtggatctga tcaacacccg cgatcgcctg
     6301 ctggcatttc tcgatcaaga gatgtcagat tctctctcct atcacttctg gaacagtggc
     6361 gtagtgattc gtcacaacga agagtacgag aagatcgaag gctgtgacga tggtgtgatc
     6421 atgcatctga agtcgggtaa aaaactgaaa gctgactgcc tgctctatgc caacggtcgc
     6481 accggtaata ccgattcgct ggcgttacag aacattgggc tagaaactga cagccgcgga
     6541 cagctgaagg tcaacagcat gtatcagacc gcacagccac acgtttacgc ggtgggcgac
     6601 gtgattggtt atccgagcct ggcgtcggcg gcctatgacc aggggcgcat tgccgcgcag
     6661 gcgctggtaa aaggcgaagc caccgcacat ctgattgaag atatccctac cggtatttac
     6721 accatcccgg aaatcagctc tgtgggcaaa accgaacagc agctgaccgc aatgaaagtg
     6781 ccatatgaag tgggccgcgc ccagtttaaa catctggcac gcgcacaaat cgtcggcatg
     6841 aacgtgggca cgctgaaaat tttgttccat cgggaaacaa aagagattct gggtattcac
     6901 tgctttggcg agcgcgctgc cgaaattatt catatcggtc aggcgattat ggaacagaaa
     6961 ggtggcggca acactattga gtacttcgtc aacaccacct ttaactaccc gacgatggcg
     7021 gaagcctatc gggtagctgc gttaaacggt ttaaaccgcc tgttttaaaa ctttatcgaa
     7081 atggccatcc attcttgcgc ggatggcctc tgccagctgc tcatagcggc tgcgcagcgg
     7141 tgagccagga cgataaacca ggccaatagt gcggcgtggt tccggcttaa tgcacggcag
     7201 ataaacaacc ccatcgcgtt tgcgctccgg cggcacagcc agcgctggca gtaaagtgat
     7261 cccgctacct gccgccacca tgttgcgcag agtttccagg ctggtcgcgc ggaagtgtgt
     7321 atcttcatcc gccccggctt caaaacagaa acccattgcc tgatcgcgca aacagtgacc
     7381 atcttccagc atcagcagtt tttcccctgc cagatcggcc atcggtacgc attcgcggtt
     7441 cgcccacggg tgatcttcat agatagccag caacattggc tcatcaaaca acggcacttc
     7501 aatgaatgct tcgctctctt tcaccagcgc gaggatcacg caatcgagtt tgccgctgtc
     7561 cagttgcgcc agtaactggt gggtctgtgc ttcatgcaga tacatttcca gctttggaaa
     7621 ggtctggtgc agcataggga taatatgcgg tagcaggtac ggtccaactg tgggaatcaa
     7681 accaatgtgc agcggtccgg acatcgtctc gccctgctgg cttgccatct ctttaaggac
     7741 tttcacctca cgcagcacgg tacgcgcctg atccaccagc agcattcccg cctgggtgaa
     7801 caacacttta cggctggtcc gctccagcaa catcacgccc agctcatctt ccagcttacg
     7861 aatttgcccg ctaagcgtcg gctggctaac gtggcaggaa tctgccgcac gccgaaaatg
     7921 gcggtgttca gccaatgcca ccaggtactc aagatcacga atattcatta tccatcctcc
     7981 atcgccacga tagttcatgg cgataggtag aatagcaatg aacgattatc cctatcaagc
     8041 attctgactg ataattgctc acagaaacgg agcggcacct cttttaaccc ttgaagtcac
     8101 tgcccgtttc gagagtttct caactcgaat aactaaagcc aacgtgaact tttgcggatc
     8161 tccaggatcc gctttttttt gccataaaaa agcccggcga taagccaggc tcaaatttat
     8221 acatataaat gttcttaccc taaccgagcc tgcgcaaaag caatcgcctg cgccacctgc
     8281 tgcggtgaga cgccgccttt tgccgcacgc ttgtcgaggc acgattgcag cgacagaatc
     8341 ggatagacat cttcgtcaat cacctgactg aatttctgca actcactgag cggcagatct
     8401 tccagcggtt tgccctgacg aatggcttcc accaccgctt caccaacaat atggtgcgcc
     8461 tcgcggaacg gtacgccttt cgccaccaga taatccgcca gttcggtggc gttggcgtaa
     8521 ccctgctgag ccgcttcctg gcaacgtgga cgtttcacct gaatgccgtc cagcaccagc
     8581 gccgccatat gcaggcagtc cagccaggta tcgagcgcgt cgaacagacc ttctttgtct
     8641 tcctgcatat ctttgttgta agccagcggc aaacctttca gcgtcatcat catgccggtt
     8701 aacgccccct gcacccggcc gcatttaccg cgaatcagct ccagcgcatc cgggtttttc
     8761 ttctgcggca ttaatgatga accggaagtc acgcggtcag aaagctccac aaaccccgct
     8821 tcgccggtgt taaagaaaat cagatcttca gcaaaacgcg acagatgcac catgccgata
     8881 gcggcagcag aaagcagttc caacacatgg tcacggtcag aaacgctgtc gagactgtta
     8941 cgggtcgccg aagcaaagcc cagccagcct gctaactgtt cacggtcgat ttcataggcc
     9001 gttcccgcca gcgcgccaca gcctagcggg ctgacatcca gacgcttaag cgcatcctgc
     9061 aaacggcttt catcacgcgc cagcatctca acataggcca ggcaccagtg cgcgaacgtc
     9121 accggctggg cgcgttgcag gtgagtgtaa cctggcatta ccgcgtcctg attgttttgt
     9181 gcggtttcca ccagcgccga ttgcagctgc cggttagccg tcagtaactc gctaacggta
     9241 tctttgcacc acagtttcag gtcagtcgct acctgatcat tacggctacg cccggtatgc
     9301 agctttttgc ctaactggcc cactttgtcg atcagtttgc cttccaccca gctatggata
     9361 tcttcggcgt cgctttcaag gatttgttgt ggcctggcgc gaacatcttc cagcaacacg
     9421 ttcagcgcct cttccagttg cgcctgctct tctgcggtta acacgcctac cgtgaccagg
     9481 gctttggacc aggccacaga gccaacaata tcctgctccg ccagacggta atcaaagcgc
     9541 agtgagtcgt tgaattgttt gaaccgttga tctgctgcct gggtaaaacg cccgccccaa
     9601 agtgccataa ctctgtttcc ttattttgaa attcaatgcc ggaaagctgc gcctccggcc
     9661 aacaaaactt aagctaaaat ccgcgtaccc atcggcatac cgttaaacag tgccggaagc
     9721 tgctccgcat gacgccagga ggcgatatct accggacggc ccagcgtgcg ggccgcatcc
     9781 agcgccgcgt tcactttcac tatcatgccg tcagtaataa tgccctgctc aatcagttgt
     9841 tctgctttcg cggcggtcat ttcggcaatg cgttgccctt tgccgtcgag aatgccgctg
     9901 acgtcggaga gcaaaatcag atccgcgccc agcgttgccg ccagcgccgt tgccgcctgg
     9961 tcggcattga cgttcatcag ttgcccttcg tctgttacgc caatggagct gaccaccggc
    10021 agataaccgt tctccagcaa ggagttgata agcttaggcg aacctggctg cgccagtcca
    10081 acatgaccta actcttcatc aagctgggtc actttgacgc tgtcgccgtc accgagaaac
    10141 aaacctacgg ccgcaatctg atgtttcttc gcccatgcca acagggtttt atttgccgtt
    10201 cccgccagtg ctccggtgat aatgtctatc tgatcagcag gcgtcacccg caggccgttt
    10261 ttctttttca ccggcagatt cagccctttc atcagctcat ccaccacgca accgccgccg
    10321 tgcacaatca ccagcggacg ctgatgtgac tcacgataat tcaccagtgc gctaaacaga
    10381 cgttccagcg cctcttcact atccagcagt acgccgccca gtttgataat taatggattc
    10441 atcattgcac ccttaaataa gagactgcgt ttccgcatag ccgaaacgaa tattggcgca
    10501 ctgtaccgct tgtgccgccg cgcctttcag taagttgtct tcggtcgcca caataatcag
    10561 atgctcgccc tgaacggcaa acccgatatc gcaaaatggc agcccaacga catttttcag
    10621 cgccggaacg cctttgtcat acagccgcac cagcggttta tgggcatacg cctgctgtaa
    10681 cacttgcgcg acttgcgcct gggtcacacc cgatttcagg cggcaggtaa tggtttcgag
    10741 aatgccgcgc gggaaattgc ccagatgtgg ggtgaagata acgtcagcac cgaggtgtgt
    10801 ggcgatctct ggttgatggc gatgagtaaa gacgccatac ggttgcaggc taacttcaca
    10861 aaagctgttt gaaatggccg ctttacgccc tgcaccgctc acgccgctgg tggcgttgat
    10921 caccggccac tgattgaggt caagaagatc ggcatcaatc aacggtttca gcgccagctg
    10981 tgccgccgtc ggataacagc ccggcaccgc aatcaaattc gcttctttta atttattacc
    11041 gcaccactcc gccagaccgt aggctgcctg ttccaacagt tccgggtatt gatgggtaaa
    11101 gccgtaatat ttttcataga aggtggcgtc gttaacacga aacgcgccgg aaaggtcgaa
    11161 caccacgcag cccgcttcaa gaaactgcgg cgctaaatcg tggctaactt catgggcggt
    11221 ggcgagaaac actacgtcca cccctgggct aaactcgctg atatccgaca tcggctgcaa
    11281 cggcagatca acgatgcctt ttagctgcgg atgcaaatcg gagattaact ttcccgcatc
    11341 attgctttgc gctgaaacag tcaaagcggt tatgttcata tgcggatggc gatttacata
    11401 ggtcactagc tctgcgccag cgtagccgct ggcacccaca atcagcgtat tcaacatcgg
    11461 ggctattcac cttcttatgt ctggttgcca ggttaaacgt aaaacattca ccttacggct
    11521 ggtgggtttt attacgctca acgttagtgt atttttattc ataaatactg catgaatatt
    11581 gatactatca tgaccagagg tgtgtcaaca atgaaaaaca aattaccgcc atttatcgag
    11641 atttaccgcg ctctgattgc cacaccttca ataagcgcca cggaagaggc actcgatcaa
    11701 agcaatgcag atttaatcac tctgctggcg gactggttta aagatttggg cttcaatgtg
    11761 gaagtgcagc ctgttccagg aactcgcaac aaattcaata tgctggcaag tatcggacag
    11821 ggggctggcg gcttgttgct ggcggggcat accgatacgg tgccatttga tgacggtcgc
    11881 tggacgcgcg atccgtttac actgacggag catgacggca agctttacgg cttaggcacc
    11941 gccgacatga aaggcttttt tgcgtttatc cttgatgcgc tacgcgatgt cgacgtcacg
    12001 aaactgaaaa aaccgctcta cattctggcg actgctgatg aagaaaccag tatggccgga
    12061 gcgcgttatt ttgccgaaac taccgccctg cgcccggatt gcgccatcat tggcgaaccg
    12121 acgtcactac aaccggtacg cgcacataaa ggtcatatct ctaacgccat ccgtattcag
    12181 ggccagtcgg ggcactccag cgatccagca cgcggagtta acgctatcga actaatgcac
    12241 gacgccatcg ggcatatttt gcaattgcgc gataacctga aagaacgtta tcactacgaa
    12301 gcgtttaccg tgccataccc tacgctcaac ctcgggcata ttcacggtgg cgacgcttct
    12361 aaccgtattt gcgcttgctg tgagttgcat atggatattc gtccgctgcc tggcatgaca
    12421 ctcaatgaac ttaatggttt gctcaacgat gcattggctc cggtgagcga acgctggccg
    12481 ggtcgtctga cggtcgacga gctgcatccg ccgatccctg gctatgaatg cccaccgaat
    12541 catcaactgg ttgaagtggt tgagaaattg ctcggagcaa aaaccgaagt ggtgaactac
    12601 tgtaccgaag cgccgtttat tcaaacgtta tgcccgacgc tggtgttggg gcctggctca
    12661 attaatcagg ctcatcaacc tgatgaatat ctggaaacac ggtttatcaa gcccacccgc
    12721 gaactgataa cccaggtaat tcaccatttt tgctggcatt aaaacgtagg ccggataagg
    12781 cgctcgcgcc gcatccggcg ctgttgccaa actccagtgc cgcaataatg tcggatgcga
    12841 tacttgcgca tcttatccga ccgacagtga ctcaaacgat gcccaactgt aggccggata
    12901 aggcgctcgc gccgcatccg gcgctgttgc caaactccag tgccgcaata atgtcggatg
    12961 cgatacttgc gcatcttatc cgacctacac ctttggtgtt acttggggcg attttttaac
    13021 atttccataa gttacgctta tttaaagcgt cgtgaattta atggcgtaaa ttcctgctat
    13081 ttattcgttt gctgaagcga tttcgcagca tttgacgtca ccgcttttac gtggctttat
    13141 aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca atgcgacgtg aaggatacag
    13201 ggctatcaaa cgataagatg gggtgtctgg ggtaatatga acgaacaata ttccgcattg
    13261 cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga
    13321 gaacacattc ttgaacgcgt agaaactatc cgtaagttgt cgaaatcttc acgcgctggc
    13381 aatgatgcta accgccagga gttgctcacc accttacaaa atttgtcgaa cgacgagctg
    13441 ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac
    13501 cacagcattt cgccgaaagg cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg
    13561 cgtaaactga aaaaccagcc ggaactgagc gaagacacca tcaaaaaagc agtggaatcg
    13621 ctgtcgctgg aactggtcct cacggctcac ccaaccgaaa ttacccgtcg tacactgatc
    13681 cacaaaatgg tggaagtgaa cgcctgttta aaacagctcg ataacaaaga tatcgctgac
    13741 tacgaacaca accagctgat gcgtcgcctg cgccagttga tcgcccagtc atggcatacc
    13801 gatgaaatcc gtaagctgcg tccaagcccg gtagatgaag ccaaatgggg ctttgccgta
    13861 gtggaaaaca gcctgtggca aggcgtacca aattacctgc gcgaactgaa cgaacaactg
    13921 gaagagaacc tcggctacaa actgcccgtc gaatttgttc cggtccgttt tacttcgtgg
    13981 atgggcggcg accgcgacgg caacccgaac gtcactgccg atatcacccg ccacgtcctg
    14041 ctactcagcc gctggaaagc caccgatttg ttcctgaaag atattcaggt gctggtttct
    14101 gaactgtcga tggttgaagc gacccctgaa ctgctggcgc tggttggcga agaaggtgcc
    14161 gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc gcctgatggc gacacaggca
    14221 tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac cagaaggcct gctgacacaa
    14281 aacgaagaac tgtgggaacc gctctacgct tgctaccagt cacttcaggc gtgtggcatg
    14341 ggtattatcg ccaacggcga tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta
    14401 ccgctggtcc gtattgatat ccgtcaggag agcacgcgtc ataccgaagc gctgggcgag
    14461 ctgacccgct acctcggtat cggcgactac gaaagctggt cagaggccga caaacaggcg
    14521 ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc
    14581 gccgaaacgc gcgaagtgct cgatacctgc caggtgattg ccgaagcacc gcaaggctcc
    14641 attgccgcct acgtgatctc gatggcgaaa acgccgtccg acgtactggc tgtccacctg
    14701 ctgctgaaag aagcgggtat cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc
    14761 gatgatctga acaacgccaa cgatgtcatg acccagctgc tcaatattga ctggtatcgt
    14821 ggcctgattc agggcaaaca gatggtgatg attggctatt ccgactcagc aaaagatgcg
    14881 ggagtgatgg cagcttcctg ggcgcaatat caggcacagg atgcattaat caaaacctgc
    14941 gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg gcggttccat tggtcgcggc
    15001 ggcgcacctg ctcatgcggc gctgctgtca caaccgccag gaagcctgaa aggtggcctg
    15061 cgcgtgactg aacagggcga gatgatccgc tttaaatatg gtctgccaga aatcaccgtc
    15121 agcagcctgt cgctgtacac cggggcgatt ctggaagcca acctgctgcc accacctgag
    15181 ccgaaagaga gctggcgtcg cattatggat gaactgtcag tcatctcctg cgatctctac
    15241 cgtggctacg tacgtgaaaa caaagatttt gtgccttact tccgctccgc tacgccggaa
    15301 caagaattgg gcaaactgcc gttgggttca cgtccggcga aacgtcgccc aaccggcggc
    15361 gtcgagtcac tacgcgccat tccgtggatc ttcgcctgga cgcaaaaccg tctgatgctc
    15421 cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg tcgaagacgg caaacagagc
    15481 gagctggagg ctatgtgccg cgattggcca ttcttctcga cgcgtctcgg catgctggag
    15541 atggtcttcg ccaaagcaga cctgtggctg gcggaatact atgaccaacg cctggtagac
    15601 aaagcactgt ggccgttagg taaagagtta cgcaacctgc aagaagaaga catcaaagtg
    15661 gtgctggcga ttgccaacga ttcccatctg atggccgatc tgccgtggat tgcagagtct
    15721 attcagctac ggaatattta caccgacccg ctgaacgtat tgcaggccga gttgctgcac
    15781 cgctcccgcc aggcagaaaa agaaggccag gaaccggatc ctcgcgtcga acaagcgtta
    15841 atggtcacta ttgccgggat tgcggcaggt atgcgtaata ccggctaatc ttcctcttct
    15901 gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac ttctgttcta acaccctcgt
    15961 tttcaatata tttctgtctg cattttattc aaattctgaa tataccttca gatatcctta
    16021 aggaattgtc gttacattcg gcgatatttt ttcaagacag gttcttacta tgcattccac
    16081 agaagtccag gctaaacctc tttttagctg gaaagccctg ggttgggcac tgctctactt
    16141 ttggtttttc tctactctgc tacaggccat tatttacatc agtggttata gtggcactaa
    16201 cggcattcgc gactcgctgt tattcagttc gctgtggttg atcccggtat tcctctttcc
    16261 gaagcggatt aaaattattg ccgcagtaat cggcgtggtg ctatgggcgg cctctctggc
    16321 ggcgctgtgc tactacgtca tctacggtca ggagttctcg cagagcgttc tgtttgtgat
    16381 gttcgaaacc aacaccaacg aagccagcga gtatttaagc cagtatttca gcctgaaaat
    16441 tgtgcttatc gcgctggcct atacggcggt ggcagttctg ctgtggacac gcctgcgccc
    16501 ggtctatatt ccaaagccgt ggcgttatgt tgtctctttt gccctgcttt atggcttgat
    16561 tctgcatccg atcgccatga atacgtttat caaaaacaag ccgtttgaga aaacgttgga
    16621 caacctggcc tcgcgtatgg agcctgccgc accgtggcaa ttcctgaccg gctattatca
    16681 gtatcgtcag caactaaact cgctaacaaa gctactgaat gaaaataatg ccttgccgcc
    16741 gctggctaat ttcaaagatg aatcgggtaa cgaaccgcgc accttagtgc tggtgattgg
    16801 cgagtcaacc cagcgtggac gcatgagtct gtacggttat ccgcgtgaaa ccacgccgga
    16861 gctggatgcg ctgcataaaa ccgatccgaa tctgaccgtg tttaataacg tggttacgtc
    16921 tcgtccgtac accattgaaa tcctgcaaca ggcgctgacc tttgccaatg aaaagaaccc
    16981 ggacctgtat ctgacgcagc cgtcgctgat gaacatgatg aaacaggcgg gttataaaac
    17041 cttctggatc accaaccagc agacgatgac cgcccgcaat accatgctga cggtattttc
    17101 gcgccagacc gacaagcagt actacatgaa ccagcaacgt acgcagagtg cgcgtgaata
    17161 cgacaccaac gtgctgaagc cgttccagga tgtgctgaat gaccctgcgc cgaagaaact
    17221 gatcatcgtt catctgctgg gtacgcatat caaatacaaa taccgctacc cggaaaatca
    17281 gggcaagttt gatggcaata ccgatcatgt tccgccagga ttaagcgcag aagagctgga
    17341 atcatataac gattatgaca acgctaacct gtataacgat catgtggttg ccagcctgat
    17401 taaagacttt aaagcggcag acccgaacgg attcctggtt tacttctctg accacggtga
    17461 agaggtttac gacacgccgc cgcacaaaac tcaggggcgt aacgaagaca acccgacgcg
    17521 ccacatgtac accattccgt tcctgctgtg gacgtcggaa aaatggcaag cgactcatcc
    17581 ccgtgatttc tcacaggatg tcgatcgtaa atacagcctg gcggaactga tccacacctg
    17641 gtcagatttg gcgggcttat cttacgacgg ttacgatcca acccgttcag tggtgaatcc
    17701 gcagttcaaa gaaactaccc gctggattgg taacccgtac aagaaaaacg cgctgatcga
    17761 ttacgacact ctgccgtatg gcgaccaggt aggtaatcag taattatcag ccaggcgcta
    17821 tttgccggat gcgacgcttg acgcgtctta tccggcctac agatcgcggc ggtaggctgg
    17881 ataaggcgtt tacgccacat ccggcaatac aactgccgca atgtttacat cgaactcccc
    17941 tcaggccaca cttttcaggt ggcctttttt aataatgcca gttactgttt tcccgtctac
    18001 atacctaagg gaataacatg tatcacgacg tcagctacct gctctcccgc ctgatcaacg
    18061 gcccgctgtc cctgcgccag atttactttg ccagcagtaa cggcccggtg cccgatcttg
    18121 cgtatcaggt cgattttcca cggctggaaa ttgtgctgga aggtgagttt gttgataccg
    18181 gcgctggagc aacgttagtt cccggcgatg tgctgtacgt tgccgctggt ggctggaatt
    18241 tcccacaatg gaaaacaccc gctaccacct ttagcgtgct gtttggcaaa cagcaactcg
    18301 gcttcagcgt cgtgcaatgg gatggcaaac aatatcaaaa tctggcgaag caacacgtcg
    18361 cccgacgcgg cccacgtata ggttcttttc tgctacaaac gctcaatgaa atgcaaatgc
    18421 agccgcagga gcagcaaacg gcaaggctta tcgtagccag cctgcttagc cactgccgcg
    18481 atttgctcgg cagccaaatc cagaccgcct cacgcagcca ggcactattt gaagctattc
    18541 gcgattatat cgacgaacgc tatgcctccg cgcttacccg cgaatctgtc gcacaggcat
    18601 tctatatttc accaaactat ctctcgcacc tgtttcaaaa aacgggggcc attggtttta
    18661 acgaatacct gaatcacacg cgactggagc acgctaagac gttattgaaa ggctatgacc
    18721 tgaaggtaaa agaagtggcg cacgcctgcg gctttgtcga cagcaactac ttctgccgtc
    18781 tgtttcgtaa aaacaccgaa cgctcgccgt cggaatatcg ccggcagtac cacagccaac
    18841 tgacagaaaa accgactact ccagaataag atgggtttgc tgcggcgcag aaagcacttt
    18901 gcgcaccgcg ctcattaccc gctgcggctc acgcaggaat gcgtagatgc tgcattgcac
    18961 atagcggcaa tgttcaaatc gctcggtacc ggcaagctcg atatccgtaa ttaacagcgc
    19021 aacatccgcc cggcggatat cctcgtccgc taaacgattc tccgttccca acgcgccctg
    19081 agtttcaatg ctgacgcccc acttctctaa ctggcacaac ttttccagcc gttccgccgc
    19141 catatatgta tgcgccacgc cgcttacgca ggcggttact gccaccaggt atgccatttt
    19201 aacctcccac ggtaacctga aatccggccc gttctgccat ttcgcgcatc gttgccacat
    19261 cggctgacga cggcgcaggc acctctttca tcgaccatgt tttccccagc aggcggtatt
    19321 tcggttcgcc gtactgatga aacggtaaca gatggatctg cctgatattc agcgggatca
    19381 gcacgtccag cgcctgctgc atattctccc ggctgagtgt gaaaccgggg atcagcggca
    19441 aacgcgggat cacgttgaca ccctcactca ccagcaaacg cagattctcc agcacgcgtg
    19501 gcaggttcat cttcaccaca tcccgcgcct gagtcgcgtc cataattttt aaatcgaaca
    19561 gcacttcatc gcacaatttc gccagcggta acagcttgct tgctggcgcg tctccggcag
    19621 tttcaatggc gcatgagaca ccccacagcc gcagtcgctg taaaaaacgg gtagcaaact
    19681 ccgcctgcat taacacttcg ccgccagaaa gcgtgacgcc gccgccggac gtgcgaaaga
    19741 aaatgtcatc tttcatcact tcccgttcca gagcgtcaag gctgatatcg cgaccaatcc
    19801 gttcaaacgc cccggagggg cattcatccg catcacgcaa acatttcgca cagtgcagac
    19861 atttcgcctc tctgcgtacc gtctggattt tgccggagat cgactccgga ttagcacacc
    19921 acgggcaaag atgcggacag cctttaaaaa agaccaccgt acgaatgccc tcgccgtcat
    19981 tcagtgaata acgctgaatg ttgaaaatgc gcgccacatc atcgcggcgc gtttccacaa
    20041 cgttacagct gatgcgctgt ccggcggatg atgtcatcct ggatctcctt cgacagttcg
    20101 acaaagaagg cgctgtatcc ggcaacgcgc accaccagcc cggcataatc ttgtgggcgc
    20161 tgttgcgctt cccgcaacgt gtcggcgttc accacgttaa actgaatatg ttgtaactta
    20221 agctgggtaa acgcccgtaa gaagtcggcc agtttgcgta atcctgcttc accttccagg
    20281 gtcgccggag tgaatttcac gttcagcaac gtaccgttag acagcagcgt gttatcgagc
    20341 ttactgactg acttcagtac cgctgttggc ccttgtgcgt cctggcccag catgggtgac
    20401 aagccgccgt ctgccagctg ttctccggca aaacgaccgt ctggcgtcgc gccaaccacc
    20461 gatcccaacg ggacgtgagc agaaacggta tacgatcccg gcgtgaagta gccgccgcgc
    20521 gggttctggt atttttccac ttctttgcag tagtggcgca acagttcggc gctgatgtta
    20581 tccacctcgt cgatatcgtt accgtatttc tcgaagcggt taattaagcg agcgcggact
    20641 ttttcgcctt ctggcgttgc gaagttggct tttaataccg acagcaattc gtcaaaactt
    20701 aaacgctgtt gctcaaaaac catccctttc aacgcatgga gagaatcgct caggttggcg
    20761 ataccgatcc cctgtacgcc ggagaagtta taacgcgcgc cgccatcggt aatatcgcgg
    20821 cctttttcca gacaatcgct gataaacgat gagagcagcg gtacaggtgc ccagtcgcga
    20881 tggccgatat cacaaatatt gctgccctca accatcaggg tgatgtagtg gctgatcttg
    20941 gcgcggatct gctccagtaa accttcataa gtcagcgcag cattgccttc attttcatgc
    21001 aggcagattt ccatcacttt cagcagatta aacatcgcga tgtcatgcaa gccgtaggtt
    21061 ctgccgggaa tagataattc cacacagccc actacggaat agtcgcgcgc atcttccagc
    21121 gaaacgccac ggttgaggaa cgctggcacc accacttcat cgttaaagat ttgcggaata
    21181 ccggtgccga agcgaatggt ttcggcggtt ttcatcagga acggcgtgtc gatcaaggcg
    21241 ttagtgcgca cgccgaggtt cggttgcggt aattgcacgc tttgataggc gtcaaggcaa
    21301 aggaacgaaa gcacgttcac cgcgctacgt ccgttctcgg ttaacccgcc gagcagtgcg
    21361 gtatagccgg tcgggaaacc ggcgaaataa cgcgcgctac tggtggagcg caacagcacg
    21421 atgtcgttgc atttcaccca taaagattcg agcagttctt tcaggaacgc cgcatcttcg
    21481 ccctgggtta atgatgtctg atagaacggc aacatatact ggtcgaagcg ccccaacgat
    21541 agcgaactgg cgttggattc gtattgcaga atgatgttca tgtaccagaa taactggcac
    21601 gcctgccaga acgtctgcgg cttatgttgc gcgttatggc gggagatctc tgcaatagtc
    21661 agcagctctt cgcgacgctg ggcatctgtg cagtttgccg ccatcgtttc cgccagttcg
    21721 gcgtaacgca aaatgtgttt ctgcgaggct tccagcagta acaacgcggc ctgataaaag
    21781 tgattctccg gctgttgctg acaatgttgc tgcatctgtg ccaccagctc acccagcccg
    21841 tgattcaaca ggcgtgggta atcaataata atgtgcccct ggcctttatc cgtctggttg
    21901 atgctgaaaa tctgcgtgtt ggtcgcggct tttacttcat ctgtcatctg cccgttgatg
    21961 aaatctttca tcgaacgttt ttcccagtac gggaacaact cttcacggta gatacgttta
    22021 tcttcttcgc tgatagcaaa gcggtcctgc ggacgcgtcg ggaattgatc cagctctttc
    22081 agcagccagt aagggtccat ttccggcgac ataatcccgg cgcgcggttt tacggtgcgg
    22141 ttaccggcaa tcagttcttc atcacgaatc gaaatttcaa catgttcaag gatatacgct
    22201 gttgctttcg cccggcgcaa tatcaccggt tcgccttcgg tttgccgatg gctggcggta
    22261 taaagcagcg cccgctccag cgagatttca cgggtattgg caaacagtgc agttttgagg
    22321 cgagagatac gattcgtcat gaggaactcc tgtgggaaag cgaacccgcc cttgcgagcg
    22381 ggagaagaac ttaagcggtt tgcgccaggt gagcttcaat tttgctcata atggcgtcag
    22441 cacgttttac tgcatcgctg atgttgacgc gcacgatggt cttgccagca aaacgctctt
    22501 caaacttgat gccgatatct ttggtcagaa tcaccatatc agcgctcgcc acatcttccg
    22561 cagtcagttc attttccaga ccaattgacc cctgggtttc tactttcact tcccagcctt
    22621 tcgctttcgc ggcactttcc agcgcctctg ctgccatata ggtatgggca acacctgaag
    22681 gacatgcggt tactgcaata attttcgtca tggctcgttc ctcaattaat taatttcaaa
    22741 atccaaatcc aggtcgtctt ctttttcatc agtcgaagac ccatttttac gcgccagact
    22801 tttcagcacg ttcacacaaa cagccgtcac cactgctccg acagccactg ctgcgatata
    22861 acccagcttg ccttcaacca ccggcagcac aatcagacca ccccagcctg catagcactg
    22921 cgcaccgacc agcgccgcag ttactgcacc acaaactgaa ccgaccatga tggaaggaat
    22981 gacacgcagc ggatcggcag cggcgaaagg aatcgcccct tcagtaacgc caacacagcc
    23041 catcaccagt gccgctttgc cggtttcgcg ctcttctgcg gagaaatttt tacgaccaat
    23101 cagcgtcgcc agccccatcc ccagcggcgg gatacaaata cccacggcag cgatggccac
    23161 cacggtataa acaccctgag caacgcaaat cagcatgaag gcataggcca ctttgttaac
    23221 cggaccgccc atatcgaacg ccagcatcag acccatgatc accgccagca taacaatgct
    23281 gccctgctgc atcccctgaa gccactgagt caggctgttg gtcaacgccc ctacaggctc
    23341 gcccaagccc cacatcatga tgcctgcggt aatcagtgtg ccgacgatag gaataatgaa
    23401 gataggcatc accgagcgca gaactttatg caccggaatt ttcttcaggt aatgcaccac
    23461 gatgccgccg ataatcccgg cgatcagtgc accaaagaag cccgcaccaa agctgttacc
    23521 aacccaggca ccgatagcgc acggagccag cgcagaacgc tctgcaatgg aataaccgat
    23581 gtacgctgcg aggaaaggca ccatcagcgt caagcccgca acgccgatat caaacagttt
    23641 tttcagattt ggatcggcta cggcatccgg cactgcgcct ttgccataca acatgacgga
    23701 aaccgccagc aaaataccgc ccgataccac gaagggaatc atgtgtgaaa cgcccgtcat
    23761 taaatgctga cgggtatttt tcaggatctg caccaactca ttcataagct gctcccgggc
    23821 ttgcgaaagc ccatgtccat atttttgatg gaggaaacaa taggcaatcg caggcggagc
    23881 aaacagtgga taaaaaggcc atttactgga cttttgtaat atcagtacaa aaatgcgatc
    23941 cgcctcataa cttgcgataa accggaagca tgccgcaaaa cagcgtcttg tgagacgcgt
    24001 cgcaaattga cggttacatt acatttgtcc agcttttggc agatttgtca catggcaggc
    24061 cgttgccgtg cgctcttatc ctgtaaacag aaaaccgtac cgggagagag gcaatggccc
    24121 tgattgtgga atttatttgt gagctgccta acggcgtaca tgcgcgtccg gcaagccacg
    24181 ttgaaacgct gtgtaatact ttttcatctc aaattgagtg gcataacctg cgcactgacc
    24241 gcaagggcaa cgccaaaagc gccctggcgc tgattggcac cgatacactg gtgggcgata
    24301 actgccagtt actgatttcc ggggctgacg aacaagcagc acaccagcgc ttgagccagt
    24361 ggttacgtga tgaattcccc cactgcgatg cgccgctggc ggaagttaaa tctgacgaac
    24421 tggaaccact gccggtttca ctgaccaatc tgaatccgca aattatccgc gcccgcaccg
    24481 tgtgcagcgg tagcgcaggc ggcattctga cgccgatctc ttctttagat ctcaatgcgc
    24541 tgggtaatct tcctgctgcc aaaggcgttg acgccgagca atccgcactg gaaaacggcc
    24601 tgacgctggt actgaaaaac attgagtttc gtctgctgga tagcgacggt gctaccagcg
    24661 cgattctgga agcgcaccga tccctggctg gcgatacttc cctgcgcgaa catttactgg
    24721 caggcgtcag cgccggatta agctgcgccg aagcgattgt caccagtgcg aatcactttt
    24781 gcgaagagtt cgcccgttcc agcagcagct acctgcaaga acgtgccctg gacgtacgcg
    24841 acgtctgctt ccagttactc cagcaaatct acggtgagca acgcttcccg gcaccgggca
    24901 aactgacgca gcccgccatt tgtatggctg atgaactgac ccccagccag ttcctcgaac
    24961 tggataaaaa tcacctcaaa ggtctgttac tgaaaagtgg cggcaccacc tcacataccg
    25021 taattcttgc ccgttcgttc aacattccga cactggttgg tgtggatatt gatgccctta
    25081 ctccgtggca gcatcaaacg atttatatcg acggcaacgc cggggcgatt gtggttgagc
    25141 caggggaagc cgtagctcgt tattatcagc aagaagcccg cgtacaggac gcccttcgtg
    25201 agcaacagca tgtctggctg acccaacaag cccgtaccgc tgacggtatc cgcattgaaa
    25261 ttgccgctaa catcgctcac tccgtggaag cgcaggccgc attcggcaat ggtgcggaag
    25321 gcgttggttt gttccgcact gaaatgctct atatggatcg caccagtgca ccgggcgaaa
    25381 gcgagctgta caacattttt tgtcaggcgc tggagtctgc caacggacgc agcattattg
    25441 tgcgcactat ggacattggc ggcgacaaac ccgttgatta tctgaacatt ccggcagaag
    25501 ctaacccgtt cctcggttat cgcgcggtgc gtatttatga agagtacgcg tcgttattta
    25561 ccacacaact acggtcgatc ctgcgcgcct ccgctcacgg cagcctgaaa atcatgatcc
    25621 cgatgatctc ttcaatggaa gagatcttat gggtgaaaga aaaactggcg gaagccaaac
    25681 agcaactacg taacgaacac attccgtttg atgagaagat ccagctcggc attatgctgg
    25741 aagtgccgtc ggtgatgttc atcatcgatc aatgctgcga agagattgat ttctttagta
    25801 ttggtagtaa tgacctgacg cagtatctgc tggcggtgga tcgcgataac gctaaggtta
    25861 ctcgtcacta caacagcctg aatccggcat tcttgcgggc gctcgattac gccgtgcagg
    25921 cggtgcatcg ccagggcaaa tggattggtc tgtgcggtga gctgggagcg aaaggttccg
    25981 tgctgccgtt gctggtcggt ttagggctgg atgaactcag catgagcgca ccgtcaattc
    26041 cggcggcgaa agcgcgaatg gcgcaacttg atagccgtga gtgccgccag ttgctcaacc
    26101 aggcaatggc ctgccgtact tcgctggaag tggaacatct gctggcgcaa ttccgcatga
    26161 cccaacagga cgcaccactg gtcaccgccg agtgcatcac gctggaaagc gactggcgca
    26221 gcaaagaaga agtgctcaaa ggcatgaccg ataacctgct gctggcaggg cgctgccgct
    26281 atccgcgtaa actggaagcc gacttgtggg cgcgcgaggc cgttttctct accggtctgg
    26341 gctttagttt tgccattcca cacagcaaat cagaacacat tgagcaatcc accatcagcg
    26401 tagcgcgtct gcaagcgccg gtgcgctggg gcgatgatga agcgcaattc atcattatgt
    26461 taaccctgaa caaacacgct gcgggcgatc agcatatgcg cattttctcg cgcctcgctc
    26521 gccgcatcat gcacgaagaa ttccgtaacg cgctggttaa cgccgcctct gccgacgcta
    26581 tcgccagcct gctgcaacat gaactggaac tgtaaaagga aacatcatgg aactgtatct
    26641 ggacaccgct aacgtcgcag aagtcgaacg tctggcacgc atattcccca ttgccggggt
    26701 gacaactaac ccgagcatta tcgctgccag caaggagtcc atatgggaag tgctgccgcg
    26761 tctgcaaaaa gcgattggtg atgagggcat tctgtttgct cagaccatga gccgcgacgc
    26821 gcaggggatg gtggaagaag cgaagcgcct gcgcgacgct attccgggta ttgtggtgaa
    26881 aatcccggtg acttccgaag gtctggcagc aattaaaata ctgaaaaaag agggtattac
    26941 tacacttggc actgctgtat atagcgccgc acaagggtta ttagccgcac tggcaggggc
    27001 aaaatacgtt gctccgtatg ttaaccgcgt agatgcccag ggcggagacg gcattcgtac
    27061 ggttcaggag ctgcaaacgc tgttagaaat gcacgcgcca gaaagcatgg tgctggcagc
    27121 cagctttaaa acgccgcgtc aggcgctgga ctgtttactg gcaggatgtg aatccatcac
    27181 cctgccctta gatgtagcgc aacaaatgct caacacccct gcggtagagt cagctataga
    27241 gaagttcgaa cacgactgga atgccgcatt tggcattact catctctaaa ggagcaatta
    27301 tggaccgcat tattcaatca ccgggtaaat acatccaggg cgctgatgtg attaatcgtc
    27361 tgggcgaata cctgaagccg ctggcagaac gctggttagt ggtgggtgac aaatttgttt
    27421 taggttttgc tcaatccact gtcgagaaaa gctttaaaga tgctggactg gtagtagaaa
    27481 ttgcgccgtt tggcggtgaa tgttcgcaaa atgagatcga ccgtctgcgt ggcatcgcgg
    27541 agactgcgca gtgtggcgca attctcggta tcggtggcgg aaaaaccctc gatactgcca
    27601 aagcactggc acatttcatg ggtgttccgg tagcgatcgc accgactatc gcctctaccg
    27661 atgcaccgtg cagcgcattg tctgttatct acaccgatga gggtgagttt gaccgctatc
    27721 tgctgttgcc aaataacccg aatatggtca ttgtcgacac caaaatcgtc gctggcgcac
    27781 ctgcacgtct gttagcggcg ggtatcggcg atgcgctggc aacctggttt gaagcacgcg
    27841 cctgttcccg tagcggcgct accactatgg cgggcggcaa gtgtacccag gcggcgctgg
    27901 cactggcaga actgtgctac aacactctgc tggaagaagg cgaaaaagcg atgcttgctg
    27961 ccgaacagca tgtagtgact ccggcgctgg agcgcgtgat tgaagcgaac acctatttaa
    28021 gcggtgtagg ctttgaaagt ggtggtctgg ctgcggcgca cgcagtgcat aacggcctga
    28081 ccgctatccc ggacgcgcat cactattatc acggtgaaaa agtggcattt ggtacgctga
    28141 cgcagctggt tctggaaaac gcaccggttg aggaaatcga aaccgtagct gcacttagcc
    28201 atgcggtagg tttgccaata actctcgctc aactggatat taaagaagat gtcccggcga
    28261 aaatgcgaat tgtggcagaa gcggcatgtg cagaaggtga aaccatccac aacatgcctg
    28321 gcggcgcgac gccagatcag gtttacgccg ctctgctggt agccgaccag tacggtcagc
    28381 gtttcctgca agagtgggaa taacctactc caaactcccg gcttgtccgg gagtttgaac
    28441 gcaaaattgc ctgatgcgct acgcttatca ggcctacgca atctctgcaa tatattgaat
    28501 ttgcgtgctt ttgtaggccg gataaggcgt tcactccgca tccggcactc tgtgccagta
    28561 atctgaaact cccacgttgt tcaggaattt tttatccgct tctggacatt ttctctacag
    28621 aacaatcgtt agcccggaag gtcgaatctg tgactcttat tttcacggac gaaccagatg
    28681 aaagcttcac tggcgctgct cagcctgcta acggcattta ccagccactc cttaaaatct
    28741 cccgccgttc caccaaccgt ggtacaaatc caggccaata ccaaccttgc catcgcagac
    28801 ggtgccagac agcaaattgg cagcacgcta ttttacgatc cggcgtatgt gcagcttact
    28861 tatcctggcg gtgatgttcc gcaagaacgc ggtgtatgtt ccgatgtggt gatccgcgca
    28921 ttgcgcagcc agaaggtcga tttgcagaaa ctggttcacg aagatatggc gaagaatttt
    28981 gctgagtacc cgcaaaaatg gaagttaaag cgcccggaca gcaacatcga tcaccgtcgg
    29041 gtgcctaatc tggaaacctg gtttagccgc catgataaaa cacgccccac cagcaagaac
    29101 cccagtgact atcaagcggg cgatattgtc tcctggcgac tggataacgg gctggcacat
    29161 attggggtgg tttcagatgg cttcgcccgc gacggtacgc cgctggtgat acataacatt
    29221 ggcgcaggcg cgcaggaaga agatgtgctg tttaactggc ggatggtggg gcattatcgt
    29281 tattttgtta aataagagat cgccccacat gaagaaaaaa ttcagatcct ttttacactg
    29341 acggcttctt ttttcttacg gctcaccagc gccagtgcca gcacaatcag cacaataccg
    29401 ctaccctcaa ccgcgccagg attctcaccg agcagccacc aggaaaagag tacgccgcaa
    29461 acgggaacgg ccagtgtgct taagctggca atactggcag gcaagttttt caatacaaac
    29521 aaccataagc tccacgccag cgccgtcgcc agaatcgcac tgtaggccag cgcccagaac
    29581 acggtgggtt gccagtcaat ttcacgttgc ggtaccagta aagcgaccac actcatcacc
    29641 agcgccgcat acagcatctg ccaggatgtt aacgacaata aatccacgcg cggatgacgg
    29701 gcgtaaaggc gtttagcgac aatggcgctc gccccccaac tgacaccgga gaggatcgcc
    29761 agcatggcgc ttttcatcga agagaaatcg agttgccacg gctgcaacac caaaaataaa
    29821 ccgaaagcgg caatcagaat cgcgaaatat tgcccacgtc gcaggcgttc accgagaaac
    29881 aacgcggcga aaatcaccac ccagaacggc atggtatagc tcaggatcgc caccttcccc
    29941 gcacctccgc tgaccaacgc ccactgcgcc agaccaacca tcccgcaggt ttgtaacagg
    30001 gcaatggcta aggtgtattt aaacggtgtc gggcgcattc cgcgaccacg taataaaagg
    30061 acgatgaata aaacgagagc gccgaaaatg cagcgtaagg cggtaaagtc gaaggcaccg
    30121 atgtaactgg tgacttgctt catgaaaatc cagctataac tccagataag tgtgagcaca
    30181 accaggccac tgattgccag tgggttgctc tttcctgcgg cagacatgag agatccagtg
    30241 tgtagttttc gttcgcccac tatacgctgg taaagaacgt tcagcgactg ccagcaagca
    30301 gccgctgaac ggggtcagat tacagcaggt cgaaacggtc gaggttcatc actttcaccc
    30361 atgccgccac gaagtcttta acaaacttct cgtgggcatc gctactggcg taaacttccg
    30421 ccaccgcacg caggacggag ttagaaccaa acaccagatc cgcacggctg gccgtaaatt
    30481 tcacttcgcc ggtttcacgg tcacggcctt cgaacagctc tttcgattcg tcggtcgctt
    30541 tccactcgta acgcatatcc agcaagttca cgaagaagtc attgctcaat acgccaacgc
    30601 ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc acccagtaca cgcatgccgc
    30661 ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg ctgtgctttg tcgatcagca
    30721 gtgactcggt ggtggaaacg tccagacgag cgcgatagtt acggaaaccg tcagcaattg
    30781 gctccagcag ctcaaacatc tcaatgtcag tctgatcctg acgcgcatca acgcgacccg
    30841 gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc ggctttctca acaccaacca
    30901 caccagccag cactatgata tccgccagcg aggctttacc agactctttc tggattttct
    30961 ccagaacagg cagagcacga acggctgcgg cgttcacatc ccagtcgcgc tgcggcatta
    31021 atgccagacg cgcaccgttg gcaccaccgc gtttgtcgcc accacggaag gtagaagcag
    31081 atgcccaggc caccgatacc agctcactaa cagacagacc agaatccgca atcgcgaatt
    31141 tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg ctgcggcagc ggatcttgcc
    31201 agatcagatc ttctttcggc acttccggcc cgatgtagcg agatttcggc cccatatccc
    31261 tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt gaacgcctgc ggatcgttga
    31321 ggaaacgacg agagatcttc tcgaactcag gatcaaaacg cagcgtcagg tcggtcacca
    31381 gcattgtcgg tttacgtttc ttcgacggat caaacggatc cgggataatt tccggtgcgt
    31441 ctaccgcttc gaactggatt gcgccagccg ggctgcgggt ctgtacccac tcatacttga
    31501 acaggttctc gaagaaatag ttgctccact gggtcggcgt ctgggtccag actacttcca
    31561 gaccagaggt aatggcatct gcgccaacgc cgctgccgta agtgctcgcc caacctaaac
    31621 cttgttcttc aatcggtgca gcttctggat caggacctac atttgatgtc ggaccggcac
    31681 cgtgggtttt acccagcgta tgaccacccg caatcagcgc cacggtttct tcgtcgttca
    31741 tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc agaaagcggt tcgccgctgt
    31801 gatccgggcc ttccgggtta acgtaaatca gacccatctc ggttgcaccc agcggtgctt
    31861 tcgccagcgc ttccggatga cggtgagtca gccaggcttt ttcatcaccc cagttaacat
    31921 ccagatccgg ttcccagacg tcttcacgac cggcaccaaa accgaaggta cggaagccgg
    31981 agttttctag cgccacgtta cccgcgagga taaacaggtc ggcccaggag attttctgac
    32041 catatttctg tttgattggc cacaacaggc gacgcgcttt atcgaggctt acgttatccg
    32101 gccaggagtt cagcggtgca aaacgttgct gaccacgacc cgcgccaccg cgtccatcga
    32161 ttgaacggta agtccccgcg ccgtgccagg ccatacgaat aaacagaccg gcgtaactgc
    32221 cccagtcggc tggccaccac ggttgagatt ctgtcaacag ggctttcaga tcttttttca
    32281 ggccgtagta atctaatttg ctgaattctt tgcggtagtc aaagtcctca cccagtgggt
    32341 tagaacgatt agaatgttgg tttaacaggt caacacgaag ttgatttggc caccagtcgc
    32401 gagtggttgt gcccgccccc gcactctggt cgtgaccgcc ctgatggaac gggcatttgc
    32461 cagtggctgt ggtgttatgg atatcgtctg acgtgctcat caatgtgctc ccctctacag
    32521 tgttaccgtt acgatacaca gcgttagaga gaagttataa ttgaattaat ccacggatgc
    32581 gatagttgag atcttacata ttgttggtta aagagatgta gatcaaattg atcttaatta
    32641 agcttttttg attatgtccg aattcggaca aatgctttat aaaaaagggt tttagcttca
    32701 tatccttcaa gtaatgaggg agaaaacaag gctgataacc ttattttccc gccctcattt
    32761 cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac tgtgtgattt tcacaaaagc
    32821 cacactattt ataaaccagg tcgaaccccc agcgtatggc aaatcgcgta actcatttca
    32881 gcacggttaa gcgtatagaa gtggaaatct ttcactcctt cacggcttaa aatcttcacc
    32941 atatccatgg caatattcgc gccaaccagt ttgcgggttt cggcatcatc atccagaccg
    33001 tcgaacattt gcgccatcca cgccggaata cgcacgttgg tcatatcggc aaatttcttc
    33061 gcctgtttaa agttagatac cggcaaaatt cccggaataa tttccacatc aatgcccgcc
    33121 gatacacagc ggtcacgaaa acgcaggtag ctttcgacat cgaagaagaa ctgagtaatc
    33181 gcgcggttgg ctccggcatc cactttgcgt ttcagattaa gcaaatccgc ctgagcgctt
    33241 tttgcttccg ggtgaacttc cggatacgcc gccacggaga tatcgaaatc tgccacttct
    33301 tttaatagcg tcaccaggtc agaagcatac atttctggct taccacttcc cggcggcaga
    33361 tcgccacgca gcgccacgat atgacgaata ccgttattcc agtagtcgcg tgcaatggtg
    33421 cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg gtgccgcttc cagaccagtg
    33481 cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc gctcgccgga gttcgcgcca
    33541 taggtcaccg atacaaactt cggtttcagg ctgctaaggc gatcgatgga gttccacagg
    33601 gtctgctcca tttcactggt acgcggcggg aaaaactcga acgaaacgtt aatctgcccc
    33661 tggacttctg ccaggctctg attcagggca tcccgctggc tggcgtgaaa aaagctcata
    33721 ccttacctca tcaatcgctt gtagttatat gttgtgttgt gcacatctat ccgtttagac
    33781 gtccagatgt aaagatgaag gaaaagccga agggcgtcaa ccgaaaaatg accaatgatg
    33841 gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg taagaaaaag aaaatccggt
    33901 atgcaaagca aaccggactg caaatataaa ctaaccatgt acttattgct tcttgccgga
    33961 ttcgtcgtaa acgcttaatc cggcctaatc ctatgataat tcaatatatt tcattagaaa
    34021 ccataaaccc gaaaacatga gtaccgggca ttattaaatt tctgaaatta caacaactgt
    34081 gccagccggt tgatatccga ctgaatcgcc ccggcggtga cgtcgcgccc agcgccaggt
    34141 ccgcggatca ccagagggtt atcgcgatac cagcggcttt cgatggcaaa gacgttatcg
    34201 cacggcagca gtgatgccaa cggatgatct tcacgcaccg cttccacgcc tacacgcgct
    34261 ttaccgttgg catcgaaacg cgccacgtag cgcagcacca gccccatttc gcgggccgct
    34321 tccagccgtt gcaccatctg ctcgttcagt tcatcgccat tttcaaagaa atggtcgatg
    34381 ctgccgcctt cgcaatgagc aggcaccagc gattccacac gtacctgatc cggttcgatg
    34441 ttgtaacctg cttcacgcgc cagaatcacc agcttgcgca tcacgtcttt gccagagaga
    34501 tcgtcacgcg ggtcaggttc ggttaagccc tgctgccacg cctgatccac cagctcggta
    34561 aacggcacgc taccgtcgaa ttgcaggaac agccaggaga gcgtgccgga gaagatcccg
    34621 ctgatcgaca aaatagtatc gccgctgtcg atcagatcgc gcacggtgtg gttgatcggc
    34681 aagcccgcac cgacggtggc attgtacagc cagtgacgcc cggttttttc gaaggcgtcg
    34741 tggatctggc gatatttatt gctgtcgctg gctcccgcca gtttgttggc gctgataacg
    34801 tggaaaccgt ggctggcgaa atcaagatac tgatcagcaa gctgctggct ggcggtaacg
    34861 tccagcacca ctaaatcatc atacggatgg gcgcgcatcc acaggaacaa cgactcttca
    34921 tcctgctcaa tcgcttcatc gttgaagaag gctaacgcgc ggctggcgtc cagcccgtca
    34981 tagctcaaca ggctgcggcg gctgtccacc acacctgcca gcacaaactc aaagccggta
    35041 cgtgccgaaa gcgtgctctg ctcacgggcg aacagttcca gccaacggga accgatattg
    35101 cccttaccga acaataccag gccgatgcgt ttttctgcgc ggaagacgga ctgatgcagc
    35161 ccctgaatca ggctttcggt cgggccggtg cgcagtactg ccaccaggct gatgccgtca
    35221 tcggactgcc aggtaaattc gaccggctgg cctttcagtt gctgccagaa gcggtggcaa
    35281 tgcagcgggt tacgggtgac gcctgcaccg accatcgcca ccagcgccag cccctgacgc
    35341 aggcgcagtt cgccaggtaa tcccgcttcg tcgaggattt tcagcgcact gtcggccact
    35401 tctgaggtgt agcaaaattg cagcaactgg cgatcgttat gtacgccaac cgccagcggg
    35461 cgtacctgcg cgcgtttcag gatttggtcg atctctttat gcgccagttt gaaatcctga
    35521 ctggcgggca cctgaaactc aatcaaacag acatcatcgt ggctggtgac aatacgcgca
    35581 ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac cttgatccgg cgtgtagcta
    35641 cagcgcagtt gcaggtcgat ttcgctgcca gaaaccggct gtaaagtacg ggcgtgaaga
    35701 acgggagccg ccaggcgcgc cagttcgctg gcctcatcca gacgcagcaa cggcagcagg
    35761 caggcatctt tcactttacg cgggtcggca ctgtataccc cggcgacgtc gctccagatg
    35821 gttacgcgag aaacacccgc cagcgcaccg atttgtgtcg cggaatagtc ggaaccgtta
    35881 cgccccagca gcaccgtttc accggcgttg ttgcggctga taaatccggt caccaccaga
    35941 cgtttgcccg gatgttgcac cagcagctgt tgcagcaacg ggtaagaaag cccttcatca
    36001 acctgcggtt gtgcggcgcg ttcagcgcgt aaaaactcgc gggcatcaag ccaggccgct
    36061 ggcagccctt gttgattaag taccgcagac atcaggcgcg ccgaccacac ttcaccgtgg
    36121 cccaccactt ccgcatacac cgcatcgtta ataccactgt cgagcaacgc cgccaggcgt
    36181 tccagatcgc tgacaaaagc gctaatgagg ctatcggctt cttcagcggg tagcagaccg
    36241 ctaatcagat cgcactgata gcgacgcagc gtttgttgaa cctgatgcgc agagagacga
    36301 tcggtctggc ttagtttcaa ccagttaatc aactggttag tggtgctacc ggcggcggaa
    36361 accaccatca tatcgtcagg ctgagagtac tccgccataa tgcccgcgac acgcaaataa
    36421 cacttcacat cagccagact actgccacca aatttatgca gctgacgacc tttcgcccct
    36481 gcctgcgcaa tcacactcat ttttacccct tgtttgcagc ccggaagcca ttttccaggt
    36541 cggcaattaa atcttcgcca tcttcaatac cggtggagat acgcagcagc gtctcggaga
    36601 tcccggcggc agcacgcgct tctggtgcca tgcctgcatg tgtcatggtt gcggcgtgag
    36661 agattaaact ttccactccc cctaatgatt ccgccagcgt aaacaacgac agcccgccca
    36721 ggaaacgacg cagcgtctgc tcatcgccat ccagttcaaa actcaacatt gcgccaaagc
    36781 ctttttgctg gcgcgcggca atttcatgcc cctgattttc cggcagcgac gggtgataca
    36841 gttttttcac caacggctgg gtttgcagat atttcacaat cgcctgcgcg ttacgctgcg
    36901 ccagctccat acgcggcacc agcgttcgca acccacgtag cagcagatag ctgtcaaacg
    36961 cgccgcccgt cacgccaata ttgtttgccc accaggccaa ttcagtgaca acgtccgggt
    37021 ctttagcaat caccacgccg gccactacgt ctgagtgacc gttcagatat ttcgtgcatg
    37081 aatgcaacac cagatcggca cctaatgcca gcggattttg taatgccggg cttaagaagg
    37141 tgttatccac cacgctcacc gccccgactt cccttgccag atggcagatt ttcgcaatat
    37201 ccacgacgcg taacaatgga ttacttgggc tttctaccag taccagtttg ggtttttctg
    37261 ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac aaacaacacg cgatagcaac
    37321 cacgtttcgc caggctgtcg aacaggcgat agctaccgcc gtagcagtcg tgcggcgcaa
    37381 ccagcaaatc gccaggtttc aaaaagacgg tggttaccag atggatggct gacatgccgg
    37441 tattggtgag cactgcaccg gcaccacctt ccagttctgc cagcgcacgc tgaaccacat
    37501 cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg cggttcatta aatccggtaa
    37561 agttataggt gctggaaaga tggatcggtg ggacaacgca accatactgt tcgtcgtcat
    37621 ttaacccgct acgcactgcg atggtggcct gtttacgcgt catgtgatga agttccctgg
    37681 gctttgtcgg tgaaatgtca ggcaccagag taaacattgt gttaatggac gtcaatacat
    37741 ctggacatct aaacttcttt gcgtatagat tgagcaaatc ccaaatagcc gttaaaatta
    37801 tatgcattat cacgccgaca ggtgcattac acgatgtcac ggtaacgcct gtacggtaaa
    37861 ctatgcgggt ttacggtcag tacccacatc aactgtgtgg tctggtctca atttattgac
    37921 gaagaggatt aagtatctca tggctgaatg gagcggcgaa tatatcagcc catacgctga
    37981 gcacggcaag aagagtgaac aagtcaaaaa gattacggtt tccattcctc ttaaggtgtt
    38041 aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac aacctgcgtc acgctaccaa
    38101 cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc gggcaacctt tgccggatga
    38161 tgccgatctg cgtaaagagc gcagcgacga aatcccggaa gcggcaaaag agatcatgcg
    38221 tgagatgggg attaacccgg agacgtggga atactaaagc aaaaaagagc ggcgcggagt
    38281 ggaatcgcct gatgcgctac gcttatcagg cctacgtcat attgcaattt attgaatttg
    38341 cacgaacttg taggccggat aaggcgttca cgccgcatcc ggcataaaca acgagcacgt
    38401 tgtctgcgac ccaccgcttt ttatacatgg acgtttaact atgaaaaaca ggctgctgat
    38461 cctcagcctg ctggtttctg tacctgcctt tgcctggcag ccacaaaccg gcgacatcat
    38521 ctttcagatc tctcgctcat cgcaaagtaa agcgatccaa ctggcgaccc ataccgatta
    38581 tagccacacc ggtatgctgg tgatacgcaa caaaaagccc tacgtttttg aagcagtcgg
    38641 cccggtgaaa tacaccccgc tcaagcagtg gatcgcccat ggtgaaaagg gcaaatacgt
    38701 tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag caaaaactgg cgcaaacggc
    38761 aaaacgttat ctcggtaaac cgtatgattt cagcttctca tggagcgacg atcgccagta
    38821 ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg ggaatgcgcg tgggcgagca
    38881 gcagaagttg aaagagttcg acctgagcag cccacaagtt caggcaaagc tcaaagaacg
    38941 ttacggcaag aatattccgc ttgaggaaac ggtggtctcg ccacaagccg ttttcgatgc
    39001 gccacaactc accacggtcg ccaaagaatg gccgctgttt tcgtggtaat acgaccaaaa
    39061 ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc
    39121 ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct
    39181 gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa
    39241 cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt
    39301 tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc
    39361 cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga
    39421 aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg
    39481 caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg
    39541 ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatgacgg
    39601 ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc atcgggattg
    39661 tggtatcagt tagcgatgcc agcgaactgc cgctcaatga gctaaaagcg gtagtcgaag
    39721 tgctggatag tgagccggtg tttactcact ccgtctggcg attgctgcta tgggcggcag
    39781 attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactacgcc
    39841 aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggccagg
    39901 cggtggatct gaacagcctg aaacgctccc ccaagcaaca acaggcgctg gcggcgttac
    39961 ggcaaggcaa aatctggcgc gaccaggtcg ccacgctcga atttaatgat gccgcgttgc
    40021 aggcgctacg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact
    40081 ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa caggccaccg
    40141 ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta
    40201 ccggttccgg taaaacggag gtttatctca gcgtactgga aaacgtgctc gctcagggca
    40261 aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc
    40321 gtgaacgttt taatgccccc gtggaagttc tgcattccgg cctgaacgac agcgagcgtc
    40381 tttcggcgtg gctgaaagcg aaaaatggtg aggcggcgat tgtgatcggc acccgctccg
    40441 cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgatgaagag cacgacagct
    40501 cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc
    40561 acagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa acgttatgca
    40621 acgtccagca gaaaaaatac cgcctgctgc gcctgacccg tcgggcaggg aatgcgcgtc
    40681 cggcaattca acatgtgctg gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg
    40741 cgttaatcac tcgtatgcgc cagcatttac aggctgataa ccaggtcatt ctctttctta
    40801 accgccgtgg ctttgcgcct gcactgctgt gccacgactg tggctggatt gccgaatgcc
    40861 cacgttgcga tcactactac acgctgcatc aggcgcagca ccatctgcgc tgccaccact
    40921 gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtcc
    40981 ccgtggggct gggcaccgaa cagcttgaac agacgctcgc gccgttgttc cccggcgtgc
    41041 ccatttctcg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg
    41101 cagaagtaca tcgcggcggc gcgcggattt tgattggtac acaaatgctg gcgaaaggtc
    41161 accatttccc ggatgtgacg ctggttgcat tactggacgt ggacggcgcg ctgttttctg
    41221 ccgattttcg ctcggcagag cgtttcgctc agctttacac ccaggtcgcc ggtcgtgccg
    41281 ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca ccatccggaa catcctctgt
    41341 tgcaaacgtt gctctataaa ggctacgacg cctttgccga acaggcgctg gctgagcggc
    41401 gaatgatgca gctaccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca
    41461 atcagcacgc gccattgttc ctgcaacaac tgcgtaatct gatcctctcc agcccactgg
    41521 cagacgagaa actgtgggtt ctcggtccgg ttccggctct ggcacctaaa cgtggcggtc
    41581 gctggcgctg gcagatattg ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta
    41641 acggtacgct ggcgctcatc aatacaatac cggattcccg taaggtgaaa tgggtgctgg
    41701 atgttgatcc gattgagggt taaaccgctc acgatgcgag gcggatcgaa aaattcaata
    41761 ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt
    41821 tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt
    41881 gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt
    41941 ctctacagcg accgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
    42001 taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta tggggcgcaa
    42061 cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct gcgatccctt
    42121 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
    42181 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
    42241 caagcaaatt gatggcatgt tgttgctggg ttcaaggctg ccgtttgatg ccagcattga
    42301 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg agctggagct
    42361 gcctacagtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt atttatatga
    42421 gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta
    42481 ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata
    42541 catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agctgcttga
    42601 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
    42661 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
    42721 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
    42781 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
    42841 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
    42901 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
    42961 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
    43021 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
    43081 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
    43141 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga
    43201 aagtgaccgg aaacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc
    43261 tggaaagtcg ccagccggga gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga
    43321 aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata
    43381 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
    43441 gtcagcaaac gctacagcgc caacgtcagg cgcagcaatt agcggaacag caacgcctgg
    43501 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
    43561 caccggtaca ggcgcaaccg cgccagtcta aaccggcttc ttctcagcag ccgtaccagg
    43621 atctgctgca aactcctgcg cacacgactg cgcagtcgaa gccgcagcaa gctgcgccag
    43681 ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga
    43741 tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg
    43801 cgttcgaagg ctttgactcg aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg
    43861 gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag atggcgggtc
    43921 atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat
    43981 aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg
    44041 tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga
    44101 tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct
    44161 gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt
    44221 cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga
    44281 gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt
    44341 cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa
    44401 cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt
    44461 agaaaacact gaacttagcg cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga
    44521 catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg
    44581 atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat
    44641 cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat
    44701 gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc
    44761 gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga atgcgccgtt
    44821 catcaaagtt gaagcgacca aattcaccga agtgggctac gtcggtaagg aagtggattc
    44881 tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa
    44941 aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc tgatcccacc
    45001 tgctaaaaac aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc
    45061 attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc
    45121 cgcagcaccg atgggcgttg aaattatggc tcctccgggc atggaagaga tgaccagcca
    45181 gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat
    45241 caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga
    45301 gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat
    45361 cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca
    45421 gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg ggatggtcaa
    45481 aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct
    45541 gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgaa ctgcaggcgc tgaccaccag
    45601 cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact
    45661 gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaaac gcatcgcgga
    45721 agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacacactgt
    45781 tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaaatat
    45841 cactattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct
    45901 gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa
    45961 ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg
    46021 gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac
    46081 agcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg cactaattac
    46141 cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg
    46201 cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca
    46261 gcaagagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct ccgggctggc
    46321 actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg
    46381 gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg
    46441 tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg ggagctggta
    46501 tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat gcggcctgct
    46561 ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc gcgaaaatgg
    46621 caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct
    46681 gctgatgggc tcgctggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg
    46741 gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg
    46801 ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg gagcgttact
    46861 gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa
    46921 tatcaattaa caattgatga ttttgccaac agcccacata gcgcgatata ctgaaaattc
    46981 tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat
    47041 caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt
    47101 ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc
    47161 gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca
    47221 ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt
    47281 tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat ccaggcgatg
    47341 gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat
    47401 tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt
    47461 ctttcagaag atccgctgga tattgaatga taagaaaggc accgcaaggt gccttttttc
    47521 tgcgttacct gttggcctac acagtaaaga aattacgcgg aagatgaagc gtaatcagac
    47581 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
    47641 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
    47701 cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
    47761 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat
    47821 acctctcctg aattgcaagg cgttgatgga taaaaatcct cgtcccgatt accggtgacg
    47881 ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg
    47941 tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca
    48001 taatccacat caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc
    48061 atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga cgaggcacac
    48121 acattttaag ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta
    48181 caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc
    48241 gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg
    48301 gttgttgatt ttcttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt
    48361 tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac
    48421 cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc
    48481 ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg
    48541 tgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca
    48601 tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct ggcactttct ctacttaccc
    48661 taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct
    48721 gatggggctg atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc
    48781 tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc cattgacagg
    48841 ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg
    48901 gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc cgcttttcgg
    48961 ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt
    49021 gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca actccttcag aacaaaaagc
    49081 ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc
    49141 tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta
    49201 gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta gaacacgacc
    49261 caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata
    49321 tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct
    49381 gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg
    49441 cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg
    49501 gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg
    49561 aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc
    49621 ttatctggaa aatgactcag ggcagtgtcc atgtgaccga ttacaccaac gcctctcgta
    49681 ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa gtgctggata
    49741 ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt cagactaaca
    49801 ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg
    49861 cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct
    49921 gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc ctactgacca
    49981 ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg gtgtttatgg
    50041 caggcgcatc cattcagtgg ctgcgcgatg aaatgaagtt gattaacgac gcctacgatt
    50101 ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggcattta
    50161 ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc
    50221 gtggggtgaa cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc
    50281 gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg
    50341 atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc
    50401 gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg tgcggcctat ctcgcaggcc
    50461 tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg
    50521 agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag
    50581 cggttaaacg cgcgatggcg tgggaagaac acgacgaata atgtaaatgc cgaatgaagc
    50641 gtttatgccg catccggtag tcccgaaacg tgcgggggca accccgcaca catcaataat
    50701 ccctcccttc ccctgtgcta cacttcgcgc cattccttac tgcttagagt ttgctatgag
    50761 acgagaactt gccatcgaat tttcccgcgt caccgaatca gcggcgctgg ctggctacaa
    50821 atggttagga cgcggcgata aaaacaccgc ggacggcgcg gcggtaaacg ccatgcgtat
    50881 tatgctcaac caggtcaaca ttgacggcac catcgtcatt ggtgaaggtg aaatcgacga
    50941 agcaccgatg ctctacattg gtgaaaaagt cggtactggt cgcggcgacg cggtagatat
    51001 tgctgttgat ccgattgaag gcacgcgcat gacggcgatg ggccaggcta acgcgctggc
    51061 ggtgctggca gtaggcgata aaggctgctt cctcaatgcg ccggatatgt atatggagaa
    51121 gctgattgtc gggccgggag ccaaaggcac cattgatctg aacctgcagc tggcggataa
    51181 cctgcgcaat gtagcggcgg cgctcggcaa accgttgagc gaactgacgg taacgattct
    51241 ggctaaacca cgccacgatg ccgttatcgc tgaaatgcag caactcggcg tacgcgtatt
    51301 tgctattccg gacggcgacg ttgcggcctc aattctcacc tgtatgccag acagcgaagt
    51361 tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgta gtttctgcgg cggtgatccg
    51421 cgcattagat ggcgacatga acggtcgtct gctggcgcgt catgacgtca taggcgacaa
    51481 cgaagagaat cgtcgcattg gcgagcagga gctggcacgc tgcaaagcga tgggcatcga
    51541 agccggtaaa gtattgcgcc tgggcgatat ggcgcgcagc gataacgtca tcttctctgc
    51601 caccggtatt accaaaggcg atctgctgga aggcattagc cgcaaaggca atatcgcgac
    51661 taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt cgccgcattc agtccatcca
    51721 ctatctggat cgcaaagacc cggaaatgca ggtgcacatc ctctgattga tttgatcgat
    51781 tgagccttcc agtccttcgg gactggaatt tttttgttcg gagaacgaag ataaggcaag
    51841 tcaatcaaaa caggagaaaa acatggctga ttgggtaaca ggcaaagtca ctaaagtgca
    51901 gaactggacc gacgccctgt ttagtctcac cgttcacgcc cccgtgcttc cgtttaccgc
    51961 cgggcaattt accaagcttg gccttgaaat cgacggcgaa cgcgtccagc gcgcctactc
    52021 ctatgtaaac tcgcccgata atcccgatct ggagttttac ctggtcaccg tccccgatgg
    52081 caaattaagc ccacgactgg cggcactgaa accaggcgat gaagtgcagg tggttagcga
    52141 agcggcagga ttctttgtgc tcgatgaagt gccgcactgc gaaacgctat ggatgctggc
    52201 aaccggtaca gcgattggcc cttatttatc gattctgcaa ctaggtaaag atttagatcg
    52261 cttcaaaaat ctggtcctgg tgcacgccgc acgttatgcc gccgacttaa gctatttgcc
    52321 actgatgcag gaactggaaa aacgctacga aggaaaactg cgcattcaga cggtggtcag
    52381 tcgggaaacg gcagcggggt cgctcaccgg acggataccg gcattaattg aaagtgggga
    52441 actggaaagc acgattggcc tgccgatgaa taaagaaacc agccatgtga tgctgtgcgg
    52501 caatccacag atggtgcgcg atacacaaca gttgctgaaa gagacccggc agatgacgaa
    52561 acatttacgt cgccgaccgg gccatatgac agcggagcat tactggtaag cggttactta
    52621 tcgataaacg gcacgatgag caaatccgca ctcatcttat tgatcatccc gcgatatgcc
    52681 ggcatcaaac ggttgataaa tgagtgatga tgaccacaga caaggaggtc gcactgctct
    52741 ttttgcataa tttccagcag tgtttccggc atttctccgc gttcaatacg cagttttgtc
    52801 ttcggccatt gaatattttt cgtcagttta tacagcttgt tatccgactt attcttcaac
    52861 aattgaagaa tatcttctgt tgcagggaag tagatacccg gatacaactc gcttaagcca
    52921 tcatcaatat gaattaacgt caggtgagcg tcattatgtc tggcgagctc cagggcttta
    52981 ttcaccagta aggcatcttc ttcattcccg gaaattgcca cgccaatgtg tttataagcc
    53041 atgtttaact ccttctaaag caactccatc aagctaacga acgcagtgat aactcaaaaa
    53101 taatcatctc ggcctggcat gagaaagtga aggccgcatc aagctcaact tgcccctcca
    53161 gatccttaat cttataggcc acgctggccc cttcatctgc agaggatgca gcggcggcaa
    53221 gctctttcag tttggtcata aaagactctg cttcttcgcg ggtggcaaaa aagcgcgaaa
    53281 acttactggt acagttgtcg ttatcaatca ccgtaccgat atcaatcgca catcctttag
    53341 tactgcattt atctacgaca tctttcatag ggcacctctg tatattcgcc tctctgtcga
    53401 ttacgactta agaataatgc gccgttaagg gaagaacaaa caagtaatga tcttatggat
    53461 taccattttt tttgatattg cttaaggtga agagttcaga aggattttcc gggtattgtc
    53521 atttgcatcg tggaaataac aatcccggaa aaaaatagca gtaagagata tattaaaggt
    53581 taattacttg atttattgtc ggctttatac ttcacatcct gggtatcttt accatattta
    53641 ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac caaaatcaac
    53701 gtcgggacaa aacgccccac cgcccattgc cagacacccg gtaaaatcgc ccagttaccc
    53761 gccagcagca tccacgccac aatcatcaaa aatgcccatg cgccggaacg cccgcgatca
    53821 tgcaagcgct taacggttac tgccgctgtt ggccaaagca agcacaccag gcaaaacgcc
    53881 gcggtctgaa tatcgagtaa atttttaccc gccagtgaga aaagcaccag catgcctgct
    53941 aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt aaatgagaat
    54001 aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg attctaccct
    54061 tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa ggtactgtca
    54121 atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt tacaacaacg
    54181 gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc tggagcccct
    54241 actttcgatc tctccatcag ccagtttcga gaagacttta atagccagaa tcccagcctg
    54301 ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct cactcgtgct
    54361 gcaagtaaaa ttaacgagaa cttgtatgct tctacagcgc tggagcgcgg taccttaaaa
    54421 atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa agccgcgaaa
    54481 gcgaaagctc aggaatacat ggcagcagtg atccgcacac tcaccccatt aatgaccaaa
    54541 acacaaagcc agaaaaaact gcagtcgcta ctaacggcgg ggaaaaacaa acgttattac
    54601 accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa ggggctgacc
    54661 ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt gaataaatga
    54721 caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc tgctgccctg
    54781 cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg cgacatcctt
    54841 tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag ctggtttcta
    54901 acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca ccaccggaaa
    54961 tgtacatcga tatggcgaag cgcgaagctg aaggcagcca catcatgctg ggtgcgcaaa
    55021 acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ttctgccgct atgctgaaag
    55081 acatcggcgc acagtacatc atcatcggtc actctgaacg tcgtacttac cacaaagaat
    55141 ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga gcagggcctg actccggttc
    55201 tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa aactgaagaa gtttgcgcac
    55261 gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt gcggttatcg
    55321 cttacgaacc cgtatgggca atcggtactg gcaaatctgc aactccggct caggcacagg
    55381 ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc gctgaacaag
    55441 tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg tttgctcagc
    55501 cagacatcga cggcgcgctg gttggcggtg cgtctctgaa agctgacgct ttcgcagtaa
    55561 tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg gatttcatat
    55621 ccggcactta ctttccttaa ctcttcgcct taacgcaaaa tctcacactg atgatcctga
    55681 atttcctcgg ctgaagcacg gttaagcgtc agtagatttc gttgtgtcgc cagcaataca
    55741 aatgagttat cactctgccg taccatcgcc agcccgtagc ttcccatatg ttcccgcgcc
    55801 tcaggcactt cttccgccag catcataaat gggctgcgtt gtaccagttc gctttccgtt
    55861 acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa ccagcggctg
    55921 ctgatgttcg ccagattgtt atccagttgt tcgcgcacat caggacgaat acaagagata
    55981 tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt tgatcgccaa agaaaccgcg
    56041 cgatcgggaa ccggctggcc gtattttttg ctcataaaat cacgcgcctg ccaggccaac
    56101 caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc gttaatacga
    56161 tacgttggca tcaacagata ttgcagtgga ccattaagat cttttaaaac cacgtatccg
    56221 gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgttg attgggcaaa
    56281 cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa tttccagtaa
    56341 ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag acccgctttt
    56401 ttcatctttt tttccctgta cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat
    56461 aaaaaacccg gtcgcgagaa accaccgggt ttgataatta tcctggggaa atcagcgttt
    56521 gctgatctga tcgaacgtac cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc
    56581 gccgaactct tcatcaatgg tgaataactt cagctttgga aacgcatttt cgtacttttt
    56641 cgccacctca gcgtcgcgcg gacggtagta gtttttcgcg gcaatttcct gaccttctgg
    56701 cgagtagaga tatttcaggt aggcttccgc cacctcttta gtaccttttt tctcgaccac
    56761 tttatcgacc accgacacgg ttggctctgc gaggatagac tcactcggcg tgacgatttc
    56821 gaatttatct ttccccagtt cattcgctgc cagcagagct tcgttttccc aggcaatcag
    56881 tacatcgcca attccgcgct cgacaaaagt gttagtggag ccacgcgcgc cagaatccag
    56941 aacttcgacg tttatataca gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt
    57001 gttgtgatgc agcgcgtagc cccaggctgc caggtagttc cagcgcgcgc caccagagct
    57061 tttcggatta ggcgtgatca ccgaaacacc cggtttaatc agatcgttcc agtcatggat
    57121 ctgcttcgga tttcccttac gtaccaggaa aacaatggtg gaagtgtacg gtgcggagtt
    57181 atccggcaga cgtttgatcc actctttatc aatccgcccg cgttccgcaa ttgcgtccac
    57241 gtcataggcc agagccagcg tgacaacatc agcttcaata ccgttgatta ccgacgtcgc
    57301 ttgtttacct gagccaccgt gtgactgacg aatcaccacg ttatcaccag tttgctgttt
    57361 ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga
    57421 aacgttaaga agctgaatat cctttgccat aacgctggtt gccgccagca aaaatgttaa
    57481 ccctacgccc cacttgttca tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg
    57541 tgccagaagg ttaaccaaac attaaagaat aaaaaaagat tggctataac ttgcgggtat
    57601 atgttggggg attaaaaagg cggaagaaat ccgcctcata ttgctgacaa agtgcgcttt
    57661 gtccatgccg gatgcggcgc gaacgcctta tccggcctac aaaagtttgc aaattcaata
    57721 gattacagaa ttcatgtaga cctgataagc gaagcgcatc aggcattttt gcttctgtca
    57781 tcggtttcag gctaaaggaa tctgcctttt tccgaaatca ttaatacagt tttttcgcgc
    57841 agtccagcca gtcgcctttg aacggacgct tcatgttttc gatagcgtcg atgatgtcgt
    57901 ggtgaaccag ctgttcgttc tggataccta cgcaacgacc gccgtaacct gccagcagca
    57961 gatcgatagc gtaagcgccc atacgggaag ccagaatacg gtcgtaaggc accggagaac
    58021 caccgcgctg gatgtggccc agcacagttg cgcgggtttc acgaccggtt tctttctcga
    58081 tgaaatgcgc cagttcgtca acatcacaca tatgttcggt aatcgccacg atcgcgtgtt
    58141 ttttaccttt cgcgataccc gctttgattt cgtttaccag atcttcacgg ctgaattcaa
    58201 cttccggaac cacaacgaat tcacagccac cggcaatggc cgcagccaac gtcagatcgc
    58261 cacaataacg gcccatcact tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac
    58321 gcagacggtc gatcgcttct acaacggtgc tcagcgcagt gaagaaacct atagtgtagt
    58381 cagtgccttt gatgtcgttg tcgatagtgc caggcagacc gatgcacggg aagcccattt
    58441 cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc gataaccacc agcgcgtcga
    58501 taccacgttt tttcaggttt tcgatagcca cggcgcggat gttctcgtcg cggaattccg
    58561 ggaaacgcgc agaaccgagg aacgtaccac cacggttgat catgtcagaa acgctgtaac
    58621 ggtctagctg taccatacgg tcttcataca gacccagata gccgtcataa atgcccatta
    58681 cttccagacc ttctgtcagc gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg
    58741 gcgcatcacc gccgcttgtc aacacaccga ttttcttaat catgactacc tctgaacttt
    58801 ggaatgcaaa atgaaatctg ttgccggaag tcttcttgca catcgaagtg atccaacgaa
    58861 tgtgcaaata gtataacaat cacttcctgc tgaattgatt caggtcaggc caaatggcgg
    58921 tattttatac acaaaatgcg ggtctggctc tcttttatac tgattatgaa agcatagacc
    58981 gtttaccctc cctgggtacg acggaacagg ggtcctgatg gataattaca tccgatcccg
    59041 gaaaacgccg taaaatagcc tgctctacct gatccgccac catatgtgcc tgaaccaaag
    59101 gcagagagtc ttccatttcc aaatgaatct gaataaagcg ggtcggccct gactgccgcg
    59161 tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt cacgatatca ataatttctt
    59221 gccgttcctc atcaggcaat gcgcgatcca gtaatgactg taccgcctca tatcccatgc
    59281 gtaacgcgct atataaaata tagatgccga ttcccaatgc aaacagagca tcggcgcgat
    59341 gccagccgta ccaggacaac cccagcgcca gcagaattgc gccgttcatc ataacatcag
    59401 actggtaatg tagcatatca gcccgcaccg cctggctttg cgtccgccgc accacccaac
    59461 gctgaaacga gacaaggata atcgtacaaa ttagcgccac aattgtcacg ataaccccga
    59521 cgcctggatc tgtcatcggt gttggagata tcagatgttg aatacccgtc aaaaacagga
    59581 atagtgccga accggagata aacatacttt gcgccagcgc cgcgagggac tcagctttac
    59641 cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga atatcgcacc accaataaat
    59701 tcgtcaacga cgcgccgata tccaccagcg aatccaccag cgcggcgaga atactcaccg
    59761 acccggtata ccaccatgca aaaattttaa tcagcaatag cagcgaagcc atcgccgtcg
    59821 cagcaatcgc cgcccgactg accagccgtc cataagattg attcataaat actcccgcta
    59881 tcaactgacg ctagtataac ggaagcaaat catctgcaat gcattaagca gcaggcaaat
    59941 tgaggataaa aaaaaccccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag
    60001 gaaaacaggg tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa
    60061 cttttttgcc attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcgttt
    60121 aaaaccgctt gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc
    60181 tcaacctgac gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt
    60241 tcatcaaaat tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca
    60301 ggaggctgtt cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg
    60361 gttaaactta tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc
    60421 ggatgccagt tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc
    60481 gttgaggcca tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc
    60541 gcgattcaac gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa
    60601 caacgtaaag tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta
    60661 aacaatgaat aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga
    60721 gctgctcgag atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga
    60781 tcttctggac gacagcattg atttactttt gcttgatgta atgatgccga agaaaaatgg
    60841 tatcgacaca ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc
    60901 gcgcggcagc gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc
    60961 gaaaccgttt aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca
    61021 ctggagcgag caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt
    61081 agtgctgaat ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg
    61141 tactgagttt accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga
    61201 acatttaagc caggaagtgc tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat
    61261 gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa
    61321 aaccctgcgt ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg
    61381 cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta
    61441 cccaagctcg attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg
    61501 atgattgagc agcatgttga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg
    61561 tggcggcgtc tgtttcgggc gattgataag tgggcaccgc caggacagcg tttgttattg
    61621 gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac
    61681 tttattggtc aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa
    61741 ctggtcggtc cgttctccgt acgtgatggc gaagataatt accaacttta tctgattcgt
    61801 ccggccagca gttctcaatc cgatttcatt aacttactgt tcgaccgccc gttattactg
    61861 ctgattgtca ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca
    61921 aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag
    61981 cacccggaac tggaagcggg gccacaggaa ttccttgccg cgggtgccag ttttaaccag
    62041 atggtcaccg cgctggagcg tatgatgacc tcccagcagc gtctgctttc tgatatctct
    62101 cacgagctgc gcaccccact gacgcgtctg caactgggta cggcgttact gcgccgtcgt
    62161 agcggtgaaa gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg
    62221 atcaacgatc tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt aagcgagacc
    62281 atcaaagcca accagttgtg gggcgaagtg ctggataacg cggcgttcga agccgagcaa
    62341 atgggcaagt cgttgacagt taacttcccg cctgggccgt ggccgctgta cggcaaccca
    62401 aacgccctgg aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg
    62461 aagattgaag tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat
    62521 ggtcctggcg ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat
    62581 gaagcacgcg atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc
    62641 attcagcagc atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg
    62701 gtgatttggt tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc
    62761 gtttacgccg catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc
    62821 aggcctacac tccgggggtt attttcccca caaccgccgt gaaaaatctt cgatcttgtc
    62881 gctcagtcgg cgcttttgca tcgttcgcgt ccagcttttc gataacccgg cggcggagag
    62941 taaacggtga tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg
    63001 cacggtcaca tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc
    63061 tggcgctatc acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat
    63121 atcgctgata tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg
    63181 aattaatgcc tctccccagc ggaaaatatc gcccatataa acattacttt ccgtcaggcc
    63241 gtcggttgag aggttttcac caaacgcagg cgcaacaaac aactccgcct gttccggaaa
    63301 ttcccgcgcc cagtagagat aatgctcacg aggataatga cacagcgctc tgtctggtcc
    63361 gccgtgaacc tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa
    63421 ctcaccatca acctggattt tagcaattgc gctgggtttg ccttcgggat aagcctgaat
    63481 cttgcctgtg tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc
    63541 cattagacca tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg
    63601 aactcaatag caaaccggac gaacgttagt tcatccggcg gtggggatta aggaagcagg
    63661 tttatttcac cgtacctaaa atttcgattt tcgccgctct ggctttgact ttacgtgacc
    63721 agatagaggt aagaattggc accagaatcg aagtcacaat gactgccgtc gctaccagcg
    63781 aagttgctgc cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg
    63841 tcgctaccgc ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc
    63901 caatcaattt atcggcgata atcaacggaa taccggtcac gataattact gccacaccca
    63961 acaggatccc cagcaaacct gtctgggcaa ttacagtcaa atcaatggta ttgcccagtg
    64021 cgaaggcaaa gaatggaatc agcgtctgca ccgctttgct gaaaaattct cgtaattcag
    64081 ggtcaaggtt ccccagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat
    64141 gcggttcaaa cgaggcaatc ccggcagtgc ccagaataat catcgtcatg agcggcccgg
    64201 actccaacga catcaacaca aatgccccag cttcttcttt tgtgccgtac tgctgcatga
    64261 tggaagcgta aagtccgccg ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa
    64321 gtccggcaaa gaatccaact tcaacaccat gttccggaat aatgcgcgag gcaattgccg
    64381 caaccaccca cgcgacggca atttttgtta ccaccagcgt accggattta cgcagtaccg
    64441 ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg
    64501 gcaccgtacc ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcccccggcg
    64561 agaaggtgtg gcacagtgcg ccaaggaata gcgggacgag catcatcccc cccgggattt
    64621 tctcaatcga gcgttttatc tgcatttcca tcacctgtcg gcatattttt aagggctgat
    64681 gcgtaattcc tcaatggagc atcagtgttg atggaaacga agatagacat tcatttaatc
    64741 aataaaagtg atcaaaacca catcaattga aacgctgttt tatttttatc ggatcattgt
    64801 ttttattttg aaattgatca caaaaaaacc accgccgttg gcgatggttc attttttaag
    64861 ctgcaatgcg gcctatacgc ctcattgcag caggcggcaa atgattattt tttcgccgca
    64921 aaacgtgccg ctgcttcgtc ccagttcacc acgttccaga actctttaat gtagtccgga
    64981 cggcggttct ggaatttcag gtagtaagca tgttcccaca catccaggcc cataatcggg
    65041 aagccggaag cgccagaaat agcttcaccc atcagcggag aatcctggtt agcagtagaa
    65101 accaccgcca gtttatcgcc tttcagcacc agccatgccc agccggaacc aaagcgggaa
    65161 gctgccgctt tttcaaattc tgctttgaag ttatcaacgg agccgaagtc acgttcgata
    65221 gccgctttca ggtcaccctg cagggtggtg ccttttttca gacctttcca gaacaggctg
    65281 tggttagcgt gaccgccagc gttgttgcgc agtacggttt tcttgtctgc tggcagctgg
    65341 tccagtttgg tgatcagctc ttcaaccggc aggttggcaa attctggcag gctttccagc
    65401 gccgcgttgg cgttgtttac gtaggtctga tggtgtttgg tgtggtggat ttccatggtc
    65461 tgcttatcga agtgcggttc cagggcatcg taagcatacg gcagggatgg cagggtatag
    65521 ctcatattca tctccagtat tgtcgggcgg ccgattgtta atgccgcgta agcagttggt
    65581 tcattatagt taattaaatg atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg
    65641 tatggttttg caggaaaatg cccgagatgt gaagcaaatc acccacttaa tgccgtgatt
    65701 gccagtaaat cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca
    65761 ctcatctcgt cggagatgtg acgcgacgaa aaatgatgag gataagaaga tgagtaacgc
    65821 gattacgatg gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc
    65881 tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt
    65941 ttcgtggatt attctgccgt gggccatcag cgccctgtta ctaccgaatt tctgggcgta
    66001 ttacagctcg tttagtctct ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg
    66061 gatcggtaat atcaactacg gcctgaccat gcgttatctc ggcatgtcga tgggaattgg
    66121 catcgccatt ggcattacgt tgattgtcgg tacgctgatg acgccaatta tcaacggcaa
    66181 tttcgatgtg ctgattaaca ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc
    66241 gctgattggc gtagggattg taactcgagc cgggcagttg aaagagcgca agatgggcat
    66301 taaagccgaa gagttcaatc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt
    66361 ctctgccggg atgtcctttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc
    66421 actaggcgtc gatccactgt atgtcgctct gccaagctat gttgtcatca tgggcggcgg
    66481 cgcgatcatc aacctcggtt tctgttttat tcgtctggca aaagtgaagg atttgtcgct
    66541 aaaagccgac ttctcgctgg caaaaccgct gatcattcac aatgtgttac tctcggcact
    66601 gggcgggttg atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc
    66661 ggcgcagtat gactacatca gttggatgct gcatatgagc ttctatgtat tgtgcggtgg
    66721 tatcgtcggg ctggtgctga aagagtggaa caatgcagga cgccgtccgg taacggtgtt
    66781 gagcctcggt tgtgtggtga ttattgtcgc cgctaacatc gtcggcatcg gcatggcgaa
    66841 ttaatctttc tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt
    66901 aaacaccacc gaaaaatagt tactatcttc aaagccacat tcggtcgaaa tatcactgat
    66961 taacaggcgg ctatgctgga gaagatattg cgcatgacac actctgacct gtcgcagata
    67021 ttgattgatg gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca
    67081 cgatgcctca tcacaaaatt tatccagcgc aaagggactt ttcaggctag ccgccaaccg
    67141 ggtaatcagc ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta
    67201 acgatggcga ttcagcaaca tcaccaactg cccgaacagc aactcagcca tttcgttagc
    67261 aaatggcaca tgctgactac tttcatgctc aagctgaccg ataacctgcc gcgcctgtgc
    67321 catccccatg ctacctaagc gccagtgtgg ttgccctgcg ctggcgctaa atcccggaat
    67381 cgccccctgc cattcaagat tcagcttcag ccgctccgga caataaataa tattctgcaa
    67441 aaccagatca ttaacggaag cgtaggagtg tttatcgtca gcatgaatgt aaaagagatc
    67501 gccacgggta atgcgataag ggcgatcgtt gagtacatgc agaccattac cgcgccagac
    67561 aatcaccagc tcacaaaaat catgtgtatg ttcggcaaag acatcttgcg gataacggtc
    67621 agccacagcg actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg
    67681 atgcgccacc gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac
    67741 aaatacgttg agaagattcg cgttattgca gaaaaccatc ccgtccctga cgaatatcac
    67801 gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc
    67861 cgcagcgata ggcgatgtca gtaacgctgg cctcactgtg acgcagcaga tgtcgggctt
    67921 tcatcagtcg caggcggttc aggtaacgct gaggcgtcag tcccgtttgc tgcttaagct
    67981 gccgatgtag cgtacgcagt gaaagagaaa attgatccgc cacggcatcc caattcacct
    68041 catcggcaaa atggtcctcc agccaggcca gaagcaagtt gagacgtgat gcgctgtttt
    68101 ccaggttctc ctgcaaactg cttttacgca gcaagagcag taattgcata aacaagatct
    68161 cgcgactggc ggtcgagggt aaatcatttt ccccttcctg ctgttccatc tgtgcaacca
    68221 gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca gtgggacgga tactgcccat
    68281 ccagctcttg tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc
    68341 ggtacagcac attagtcaga cacagattat cggtatgttc atacagatgc cgatcatgat
    68401 cgcgtacgaa acagaccgtc ccaccggtga tggtataggg ctgcccatta aatacatgaa
    68461 tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat
    68521 ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca
    68581 cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca
    68641 cgaggtcagg ttcttacctt aaattttcga cggaaaacca cgtaaaaaac gtcgattttt
    68701 caagatacag cgtgaatttt caggaaatgc ggtgagcatc acatcaccac aatgaagcaa
    68761 attgtgaaca tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt
    68821 agcgagaagg tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat
    68881 cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg
    68941 atgctggcgc gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt
    69001 aacaatggcc tgcatagcca gaacggttat gtcacctgga atgtggatag cctggaaagt
    69061 gccattcgcc ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg
    69121 attgatacct ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg
    69181 cccgttgctt atcgcgatag ccgcaccaat ggcctaatgg cgcaggcaca gcaacaactc
    69241 ggcaaacgcg atatttatca acgtagcggc atccagtttc tgcccttcaa tacgctttat
    69301 cagttgcgtg cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg
    69361 ctgatgccgg attacttcag ttatcgcctg accggcaaga tgaactggga gtacaccaat
    69421 gccacaacca cgcaactggt caatatcaat agcgacgact gggacgagtc gctactggcg
    69481 tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcataggt
    69541 cactggattt gcccgcaggg taatgagatt ccggtggtcg ccgttgccag ccatgatacc
    69601 gccagcgcgg ttatcgcctc gccgttaaac ggttcacgcg ccgcttatct ctcttctggc
    69661 acctggtcat tgatgggctt cgaaaaccag acgccattta ccaatgacac ggcgctggca
    69721 gccaatatca ccaacgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg
    69781 ggcttatggc tgcttcagcg ggtgcttcag gagcggcaaa tcaacgatct cccggcgctt
    69841 atcgccgcga cacaggcact tccggcctgt cgcttcatca tcaatcccaa tgacgatcgc
    69901 tttattaatc ctgaagcgat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa
    69961 ccgatcccgg aaagtgatgc tgaactggcg cgctgtattt tcgacagtct ggcgctgctg
    70021 tatgccgatg tgttgcatga gttggcgcag ctgcgtggtg aagatttctc gcaactgcat
    70081 attgtcggcg gcggctgcca gaacacgctg ctcaaccagc tgtgtgccga tgcctgcggt
    70141 attcgggtga tcgccgggcc tgttgaagcc tcgacgctcg gcaatatcgg catccagtta
    70201 atgacgctgg atgaactcaa caatgtggat gatttccgtc aggtcgtcag cactaccgcg
    70261 aatctgacca cctttacccc taatcctgac agtgaaattg cccactatgt ggcgcagatt
    70321 cactctacac gacagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg
    70381 agctggcgaa acagcgtttc gcggcagtag ggattgatgt cgaggaggcg ctgcgccaac
    70441 ttgatcgttt acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa
    70501 acccggaagg ttcgctgacc ggtgggattc aggctactgg caattatccg ggcaaagcgc
    70561 gtaatgccag tgagctacgt gccgatctgg aacaggctat gcggctgatt cccgggccaa
    70621 aacggcttaa tttacatgcc atctatctgg aatcagacac gccagtctcg cgcgaccaga
    70681 tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa agccaatcag ctcggtctgg
    70741 attttaaccc ctcctgtttt tcgcatccgc taagcgccga tggctttacg ctctcgcatc
    70801 ccgacgacag cattcgccag ttctggattg atcactgcaa ggccagccgt cgcgtttcgg
    70861 cctattttgg cgagcaactc ggcacaccgt cggtgatgaa catctggatc ccggatggca
    70921 tgaaagatat caccgttgac cgtctcgctc cgcgccagcg tctgctggca gctctggatg
    70981 aggtgatcag cgagaagctg gatccggcgc accatatcga cgccgttgag agcaaattat
    71041 ttggtattgg tgcggagagc tacacggttg gctccaatga gttttaccta ggttatgcca
    71101 ccagccgcca gacggcgctg tgcctggacg ccgggcattt ccacccgact gaagtgattt
    71161 ccgacaagat ttccgccgcc atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc
    71221 cggttcgctg ggacagcgat cacgtagtgc tgctggatga tgaaacccag gcgattgcca
    71281 gtgagattgt tcgtcacgat ctgtttgacc gggtgcatat cggccttgac ttcttcgatg
    71341 cctctatcaa ccgcattgct gcgtgggtta ttggtacacg caatatgaaa aaagccctgc
    71401 tacgtgcgtt actggaacct accgctgaac tacgcaagct ggaagcggcg ggcgattaca
    71461 ctgcgcgtct ggcactgctg gaagagcaga aatcgttgcc gtggcaggcg gtctgggaaa
    71521 tgtattgcca acgtcacgat acgccagcag gtagcgaatg gctggagagc gtgcgggctt
    71581 atgagaaaga aattttgagt cgccgcgggt aaacactgcc ggatgcggcg cgagcgcctt
    71641 atccggccta cgggtcggca acagttgtag gcctgataag acgcgacagc gtcgcatcag
    71701 gcattgattg ccggatgcag cacaagtgcc ttatccggcc tacaggtcgg caatagttgt
    71761 aggcctgata agacgcgaca gcgtcgcatc aggcattgat tgccggatgc ggcacaagtg
    71821 ccttatccgg cctacaggtc ggcaacagtt gtaggcctga taagacgcga cagcgtcgca
    71881 tcaggcattg attgccggat gcggcacaag tgccttatca ggcctacagg tcggcaatag
    71941 ttgtaggcct gataagacgc gacagcgtca catcaggcat tgattgccgg atgcggcgcg
    72001 agcgccttat ccggcctacg ggtcgtgcat ccgacaacac cgaatttaca ggaacacaga
    72061 acatgcaaaa cattactcag tcctggtttg tccagggaat gatcaaagcc accaccgacg
    72121 cctggctgaa aggctgggat gagcgcaacg gcggcaacct gacgctacgc ctggatgacg
    72181 ccgatatcgc accatatcac gacaatttcc accaacaacc gcgctatatc ccgctcagcc
    72241 agcccatgcc tttactggca aatacaccgt ttattgtcac cggctcgggc aaattcttcc
    72301 gtaacgtcca gcttgatcct gcggctaact taggcatcgt aaaagtcgac agcgacggcg
    72361 cgggctacca cattctttgg gggttaacca acgaagccgt ccccacttcc gaacttccgg
    72421 ctcactttct ctcccactgc gagcgcatta aagccaccaa cggtaaagat cgggtgatca
    72481 tgcactgcca cgccaccaac ctaatcgccc tcacctatgt actggaaaac gacaccgcgg
    72541 tctttactcg ccaactgtgg gaaggcagca ccgagtgtct ggtagtgttc ccggatggcg
    72601 ttggcatttt gccgtggatg gtgcccggca cggacgaaat cggccaggcg accgcacaag
    72661 agatgcaaaa acattcgctg gtgttgtggc ccttccacgg cgtcttcggc agcgggccga
    72721 cgctggatga aacattcggt ttaatcgaca ccgcagaaaa atcagcacaa gtattagtga
    72781 aggtttattc gatgggcggc atgaaacaga ccatcagccg tgaagagttg atagcgctcg
    72841 gcaagcgctt cggcgttaca ccactagcca gtgcgctggc gctgtaagga gcaaacatga
    72901 tccgcaaagc ctttatcatg caggttaacc ccgacgccca cgaagagtat cagcgtcggc
    72961 ataaccccat ctggccggaa ctggaagcag tgttgaaatc tcacggtgcg cataactacg
    73021 ccatttacct cgacaaagcg cgtaatctgc tgtttgccat ggtagagatt gaatctgaag
    73081 aacgctggaa tgcggtagcc agcactgaaa tctgccaacg ttggtggaaa tatatgaccg
    73141 acgttatgcc agctaacccg gataacagcc cggtgagtag cgagctgcaa gaagtgtttt
    73201 acctgcctta atgagttgcg gtgccggaaa atatccagca ccgcgacttc atcagaagaa
    73261 gtatttaaag cgaacgcgtg cgccgtaacg gtcatcagta tcatcaccgt gttttttatc
    73321 accgtcgcta tccagaatgg agtaatacgt cccgaggtaa atattaaagt tctccatatc
    73381 catcacatta gcgaactgat aagacgcatg aatggtgtga atggtgtacg tcccttcatc
    73441 atcaatccag cagtcgttat cacaaactac accgctgaat tcatcaatct tattgtgcgc
    73501 gtagatataa ccgagctcga aacgtttcca cagcgcgtta atcccggcag tgaaatcttt
    73561 ctcattatta gcatctaaat aggcggtatt aagattaacc acaatgccat tgtccggatc
    73621 cgttttcagg ccgttccagg tcatgctcat accatagccg gtacgatcgg actgatcgac
    73681 aaaattaccc ttgctatcgg tataaccata agcattattt accacgttag cttccatcgc
    73741 tgcggaaacg gtgaattctt ccgtcggcga ccaggcaatt accggacgca gataagcaac
    73801 atttttctgc tgctccatat cgcgtccatg ataattaccg tcattatata aagatgttcc
    73861 gtcttcaagt aacgtgttta attcaaaata ccagttatcg agttgtttgc tgacgaggaa
    73921 attaccgcca gcgttagagc gcccgcggcc ctctttcatc atatagatat aaccgctgcc
    73981 atcgtcataa agatcgttcg cagtattacc ggaatgttca acaaaggtat cctgattcag
    74041 cgggaacata tcgtaggctt caaaacggcc tactttgacc ttccagtcgt tttctttgcc
    74101 aaagaagaat accgcatcat cgatatttac tgaaccgtgc atatccccca gcggttgtgc
    74161 ggagaacccg gcgaaataac cattatccag ttttcgcata ccatcaaaac ctaacagaat
    74221 acgaccattg agattccact gttcattagt gggatcgcta ttcacatcag cgttggtcat
    74281 tgccagcagg ccatgattac tttccgcatc catattaaat tctacgtcgc cgtagatttt
    74341 taaatcgcca taacctgaaa gcaaaagatt aggtgaggtg gcatttttct ttgcagattc
    74401 cgccgttgtc tgtccattta ccggcgtatt gccctgggca gccttaattt cgcggatctc
    74461 agcggcttgt tgtgtctgca gctgttttac ctgcccctct gcttttgccg cccgcatttc
    74521 tgccgcttcc aggcgttctt ccagcaatgc catgcgttgt tccagcgtta aatgctgtgt
    74581 ttttgccata ccggtactac atgccaataa taaaccgatg gctaaagata atgtcgattt
    74641 tttcattatt tacgttccct gaaataaagg aggccctacg ctttccaaaa ggaatacgta
    74701 tatagagaaa tataaggtgt gcactttcga atttttaatc caacttcagc aggccgatgg
    74761 atagtatccc gccgcataat atgagtaaat ccgctgattt cctggaacgt gaggaatata
    74821 acaatagtgc cgtttttaac taaatatcgt aaaaaatatt agatatgatt cacgctgact
    74881 cataagccat aaatataaat aacgtggcag caaaatatcc gcgacagtta aacattcata
    74941 aaatcattaa agaatgattt tttattcacg acatttattt taccaatcaa tatgtgcctt
    75001 accccgcaaa atttatcctt cactctgcct tatattgtca cgacttcacg acatatccgc
    75061 gctcatcaca aaaccgcccc gccgttacaa ttaacaataa tattttttca tggagtccaa
    75121 taatggcagc acttactgca agctgtattg acctgaatat tcagggcaat ggcgcttatt
    75181 ccgttctgaa gcagttggcg acaatagcgt tacaaaacgg ttttatcacc gactcacacc
    75241 agttcctgca aaccctgctg ctgcgcgaaa aaatgcactc tactggattt ggttccggtg
    75301 tcgccgtgcc gcacggtaaa agcgcctgcg ttaaacaacc gttcgtatta ttcgcccgca
    75361 aagcgcaggc tattgactgg aaagccagcg atggcgaaga cgtcaattgc tggatctgcc
    75421 tcggcgtgcc gcaaagcggc gaagaggatc aggtcaaaat catcggcaca ctgtgtcgca
    75481 aaattatcca ccaggatttt attcatcaac tgcaacaggg cgacgccgac caggtgcttg
    75541 ccttgttaaa tcaaaccctc agctcataag gaagtggcga tggagtcatc cttacgtatt
    75601 gtcgcgatca ccaactgccc cgccgggatc gctcacacct acatggtggc ggaagccctg
    75661 gaacagaaag cccgttctct cggccatacc ataaaagtgg aaactcaagg gtccagtggc
    75721 gttgaaaacc gcttatccag cgaagagatt gccgctgccg attacgtcat tctcgctacc
    75781 gggcgtggcc tgagcggtga tgatcgcgcg cggtttgccg ggaagaaagt ttatgagatt
    75841 gccatctccc aggcgttgaa aaatatcgac cagattttca gcgaattacc gacaaactcg
    75901 cagctttttg ccgcagatag cggcgtgaag cttggcaaac aggaagtgca aagcggcagc
    75961 gtgatgagcc acctgatggc gggcgtctct gcggcgctgc cgtttgtcat cggcggcggt
    76021 attctggtgg cgcttgccaa catgctggtg cagttcggtt tgccatatac cgatatgtcg
    76081 aaaggtgcgc cgtcgtttac ctgggtagtt gaatccatcg gttacctcgg cttcaccttt
    76141 atgatcccca tcatgggcgc ttatatcgcc tcgtcgattg ccgataaacc ggctttcgct
    76201 ccggcgtttc tggtttgcta tctggcgaac gacaaagccc tgctcggcac ccagtcgggt
    76261 gcgggatttc tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt cttctggttc
    76321 cgtaaagtgc gccttggcaa agcattacaa ccgctgctcg gttccatgct gatcccgttc
    76381 gtcactctgc tggttttcgg cgtgctcact tactacgtta tcggaccggt gatgtccgac
    76441 ctcatgggcg ggctgctcca cttcctgaat accattcctc catcaatgaa gtttgcggca
    76501 gcgtttctgg tcggtgcgat gctggcgttc gatatgggcg gccccatcaa taaaaccgcc
    76561 tggttcttct gcttctcact gctggaaaaa cacatttatg actggtacgc catcgtcggc
    76621 gttgtcgcgc tgatgccgcc cgtcgcggcg ggtcttgcga cgttcattgc gcccaaactg
    76681 tttacccgac aggaaaaaga ggctgccagt agtgccattg tggtcggtgc cacagtcgca
    76741 accgaaccgg ctattcctta cgctctggca gctccgttgc cgatgattac cgccaatacg
    76801 ctggcgggcg gcattaccgg cgtgctggtg atcgctttcg gaatcaaacg tctggcacct
    76861 ggtttgggta tctttgaccc gctgatcggc ctgatgtcgc cggtgggttc gttttatctg
    76921 gtgctggcga ttggcctcgc gctgaacatt tcattcatta tcgtcctgaa aggattgtgg
    76981 ttacgccgca aagcgaaagc cgcgcaacag gagcttgtcc atgaacattg agttactgca
    77041 acagttgtgc gaagccagcg ccgtcagcgg cgatgaacag gaagttcgcg acattctgat
    77101 aaacacgctg gaaccttgcg tgaatgaaat caccttcgat ggtctgggca gctttgttgc
    77161 ccgtaagggg aataaaggtc caaaagttgc cgttgtcgga catatggatg aagtcggctt
    77221 tatggtcacc cacatcgacg agagcggttt tctgcgtttt accaccattg gcggctggtg
    77281 gaatcagtcg atgctcaacc accgggtaac catacgcaca cacaagggag tgaaaatccc
    77341 tggtgtgatt ggttccgtcg cgcctcatgc gttaacggaa aagcaaaggc aacagccgct
    77401 gtcatttgat gagatgttca ttgatattgg cgcaaacagt cgcgatgagg tggaaaaacg
    77461 cggtgtggcg atgggcgatt ttattagccc ggaagccaat tttgcctgct ggggcgaaga
    77521 taaagtggtc ggcaaggcgc tggataaccg catcggctgc gcaatgatgg ctgaactatt
    77581 gcagacggtg aataatcccg aaattacgct gtatggcgtc ggcagcgtgg aagaagaagt
    77641 tgggctacgc ggggcacaaa cctcggcgga acacattaaa ccggatgtgg tgattgtgct
    77701 ggataccgct gtcgcaggtg atgttccagg cattgataac attaaatacc cgctgaaact
    77761 gggccagggg ccggggctga tgctgtttga caagcgctac ttccccaacc agaaactggt
    77821 ggcggcgtta aaaagctgtg ccgcacataa cgatttaccg ctgcaatttt ccaccatgaa
    77881 aaccggtgcg acggatggcg ggcgctacaa cgtgatgggc ggcgggcgtc cggttgttgc
    77941 gctgtgcctg ccaacccgct atctgcacgc taacagcggc atgatttcaa aagccgatta
    78001 ccatgctctg ctcacgttga tacgggattt tctgaccacc ttaaccgcgg agaaagtcaa
    78061 cgcgtttagc cagttccgtc aggtggatta atgctaaacg aacgccagtt aaagattgtc
    78121 gatctgctgg agcaacagcc gcgcacgcct ggtgagctgg cgcaacagac tggcgtttca
    78181 ggcaggacca tcctgcgtga tattgactat ctcaacttta cccttaacgg caaagcccgc
    78241 atttccgcca gtggtagtgc gggctatcag ctggaaatct tcgagcgccg cagctttttt
    78301 cagttgctgc aaaagcacga taacgacgat cggctgctgg cactgttatt actgaatacc
    78361 tttactcccc gtgcgcaact cgcctcggcg cttaatttgc cagaaacgtg ggtcgcagag
    78421 cgtctgcccc ggttaaaaca gcgttatgaa cgcacttgtt gcctggccag ccgccctggt
    78481 ttgggccatt tcattgatga gacagaagag aaacgcgtta tcttgctggc gaacttgctg
    78541 cgcaaagatc cgtttttaat tccgctggcg ggcataacac gagacaacct tcagcattta
    78601 tccacggcct gcgacaacca acaccgctgg ccgctcatgc agggtgatta tctctccagc
    78661 ctgattctgg cgatttacgc cctgcgtaat caactgaccg atgagtggcc gcaatatccc
    78721 ggtgacgaga taaaacaaat cgttgaacat agcggtctgt ttcttggtga taacgctgta
    78781 agaaccctga cgggtttgat agagaaacag catcagcaag cgcaggtaat ttcagccgat
    78841 aatgtgcagg ggttgctgca aagggtgccg ggcatcgcgt cattgaatat tattgatgcg
    78901 cagctggttg agaatattac cgggcattta ttacgttgcc ttgccgcacc agtgtggatt
    78961 gctgagcacc gccagagcag catgaataac ctgaaagccg cctggcctgc ggcctttgat
    79021 atgagtctgc actttattac gctactgcgt gaacagctcg atattcctct tttcgacagc
    79081 gatctgatcg gtttgtattt tgcctgtgcg ctggaacggc atcaaaacga acgccagccg
    79141 atcattttgc tctcggacca gaacgcgatt gccactatta atcagctcgc cattgagcgc
    79201 gatgttttac attgtcgggt gattattgcc cgtagcttaa gcgaacttgt tgccattcgc
    79261 gaagagattg agccgttatt gatcattaac aacagccatt atttactgga tgacgcggta
    79321 aataattaca tcaccgtaaa aaatatcatt acggctgccg gtatcgaaca aataaaacat
    79381 tttctggcga cggcatttat tcgccaacag ccggagcgtt ttttctctgc ccccggaagt
    79441 tttcattatt cgaatgtacg cggtgaaagc tggcaacata ttacccggca aatttgtgcg
    79501 caattagtgg cacaacacca tattaccgcc gatgaagcac aacgcatcat cgcccgcgaa
    79561 ggcgaaggtg aaaacctgat tgttaatcgc ctcgccatcc cacattgctg gagcgaacag
    79621 gagcgacgtt ttcgtggatt ttttattacc ctcgcccaac cagttgaggt gaataacgaa
    79681 gtcattaacc atgtcttgat cgcctgcgcc gccgccgatg cgcgtcatga gctaaaaata
    79741 tttagctatc tggcaagcgt attgtgtcag catccggcag aggttattgc cgggttaaca
    79801 ggatatgagg catttatgga gttacttcac aaggggtgaa ctgtgtcgga tgcgccgcat
    79861 ccgacactgc atttatcatc aaaaacgttt aacgcaggct attatagtca tcaaccattt
    79921 cgttgtactc gcgtaatgct ctcgggaaag agtcgtcgac catccagctt gagtttgcgt
    79981 cctgcagttg ctctttatcc cagtcagagt acgggacttt atctctgacg cggcgaacgt
    80041 attttttagc ctcgagttga tactggcctg ccgaattaat aaacgagaaa ttcatgccgc
    80101 ctttatccgc ttctttggcc tgcgccacca gcgtttccag ttcagacact ttcttcattg
    80161 ccgcttctgc atcaaactta tcttgcgata tcaggttatt aatttgtttc gctgaaatca
    80221 tgactgccag agagtaatag tggaatgttt tcccttctct ttcttcaata tttttcagtt
    80281 ccgcaagctg gcgcttatcg ttaatttcct gaatcgccgc atgataagat tctgctacag
    80341 cttcaaaggc ttccagattt tttaagaatg tctggtgcag cgttttccct ttcgcaaacg
    80401 catcgtcttt ataattttcc tgggtgtaat atttatccat ttcgttaatg gtattaccca
    80461 acgccacggc agcatcgata taactcaccg ccacgccatc aataggttgc agcgcaggcg
    80521 ttaacgccac agcacttttt acgccttttt cacactcagc gaggttggat tcactaatgc
    80581 cgtagatgcc ataaacagta cgctcttcac cggtaggccc ctgtttaaaa tctttcagcc
    80641 agtcagcata acgcgccaga ctgctctgta cagggatctg taacttgtta tagcacttga
    80701 tatagacatc cattttttcg tcgaaaaccg cttgttcatc gaccaccggt tgcgcaggtg
    80761 tttcaggttt agcttctgct tttgcaacgg gagcttcagg agctggtgcg gcaggttgac
    80821 tattggcagg cgggggcgtt tctgtttcgg cttttttatc atcacaaccc gtcagaccga
    80881 gggccatgat ggcgactata atagcggaag ataataaatt ccttttcatt taattgcctt
    80941 ataaatatat agataagaga gtgaacataa taaatgaaaa aagaaaacgc cactac
//
LOCUS       DAZFZC010000009        78168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFZC010000009 DAZFZC010000000
VERSION     DAZFZC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.009"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            341..1714
                     /gene="aroP"
                     /locus_tag="ACHKXP_000724"
     CDS             341..1714
                     /gene="aroP"
                     /locus_tag="ACHKXP_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO7645793.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1757..2611)
                     /gene="ampE"
                     /locus_tag="ACHKXP_000725"
     CDS             complement(1757..2611)
                     /gene="ampE"
                     /locus_tag="ACHKXP_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO7645794.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMVGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2608..3159)
                     /gene="ampD"
                     /locus_tag="ACHKXP_000726"
     CDS             complement(2608..3159)
                     /gene="ampD"
                     /locus_tag="ACHKXP_000726"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO7645795.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3247..4140
                     /gene="nadC"
                     /locus_tag="ACHKXP_000727"
     CDS             3247..4140
                     /gene="nadC"
                     /locus_tag="ACHKXP_000727"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO7645796.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINTNQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4343..4783
                     /gene="ppdD"
                     /locus_tag="ACHKXP_000728"
     CDS             4343..4783
                     /gene="ppdD"
                     /locus_tag="ACHKXP_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO7645797.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4793..6178
                     /gene="gspE"
                     /locus_tag="ACHKXP_000729"
     CDS             4793..6178
                     /gene="gspE"
                     /locus_tag="ACHKXP_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO7645798.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGIQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6168..7370
                     /gene="hofC"
                     /locus_tag="ACHKXP_000730"
     CDS             6168..7370
                     /gene="hofC"
                     /locus_tag="ACHKXP_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO7645799.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEAALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7405..8448)
                     /gene="guaC"
                     /locus_tag="ACHKXP_000731"
     CDS             complement(7405..8448)
                     /gene="guaC"
                     /locus_tag="ACHKXP_000731"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO7645800.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(8604..8648)
                     /locus_tag="ACHKXP_000732"
     CDS             complement(8604..8648)
                     /locus_tag="ACHKXP_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645801.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8673..9293
                     /gene="coaE"
                     /locus_tag="ACHKXP_000733"
     CDS             8673..9293
                     /gene="coaE"
                     /locus_tag="ACHKXP_000733"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO7645802.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9293..10036
                     /gene="zapD"
                     /locus_tag="ACHKXP_000734"
     CDS             9293..10036
                     /gene="zapD"
                     /locus_tag="ACHKXP_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO7645803.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIADHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10046..10243
                     /gene="yacG"
                     /locus_tag="ACHKXP_000735"
     CDS             10046..10243
                     /gene="yacG"
                     /locus_tag="ACHKXP_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO7645804.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10459..10848)
                     /gene="mutT"
                     /locus_tag="ACHKXP_000736"
     CDS             complement(10459..10848)
                     /gene="mutT"
                     /locus_tag="ACHKXP_000736"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO7645805.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10908..13613)
                     /gene="secA"
                     /locus_tag="ACHKXP_000737"
     CDS             complement(10908..13613)
                     /gene="secA"
                     /locus_tag="ACHKXP_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO7645806.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13675..14187)
                     /gene="secM"
                     /locus_tag="ACHKXP_000738"
     CDS             complement(13675..14187)
                     /gene="secM"
                     /locus_tag="ACHKXP_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO7645807.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLC"
     gene            complement(14418..15335)
                     /gene="lpxC"
                     /locus_tag="ACHKXP_000739"
     CDS             complement(14418..15335)
                     /gene="lpxC"
                     /locus_tag="ACHKXP_000739"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO7645808.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15436..16587)
                     /gene="ftsZ"
                     /locus_tag="ACHKXP_000740"
     CDS             complement(15436..16587)
                     /gene="ftsZ"
                     /locus_tag="ACHKXP_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO7645809.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16648..17910)
                     /gene="ftsA"
                     /locus_tag="ACHKXP_000741"
     CDS             complement(16648..17910)
                     /gene="ftsA"
                     /locus_tag="ACHKXP_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO7645810.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17907..18737)
                     /gene="ftsQ"
                     /locus_tag="ACHKXP_000742"
     CDS             complement(17907..18737)
                     /gene="ftsQ"
                     /locus_tag="ACHKXP_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO7645811.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18739..19659)
                     /gene="ddlB"
                     /locus_tag="ACHKXP_000743"
     CDS             complement(18739..19659)
                     /gene="ddlB"
                     /locus_tag="ACHKXP_000743"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7645812.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19652..21127)
                     /gene="murC"
                     /locus_tag="ACHKXP_000744"
     CDS             complement(19652..21127)
                     /gene="murC"
                     /locus_tag="ACHKXP_000744"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO7645813.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21181..22248)
                     /gene="murG"
                     /locus_tag="ACHKXP_000745"
     CDS             complement(21181..22248)
                     /gene="murG"
                     /locus_tag="ACHKXP_000745"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO7645814.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRVARA"
     gene            complement(22245..23489)
                     /gene="ftsW"
                     /locus_tag="ACHKXP_000746"
     CDS             complement(22245..23489)
                     /gene="ftsW"
                     /locus_tag="ACHKXP_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO7645815.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23489..24805)
                     /gene="murD"
                     /locus_tag="ACHKXP_000747"
     CDS             complement(23489..24805)
                     /gene="murD"
                     /locus_tag="ACHKXP_000747"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO7645816.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24808..25890)
                     /gene="mraY"
                     /locus_tag="ACHKXP_000748"
     CDS             complement(24808..25890)
                     /gene="mraY"
                     /locus_tag="ACHKXP_000748"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO7645817.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25884..27242)
                     /gene="murF"
                     /locus_tag="ACHKXP_000749"
     CDS             complement(25884..27242)
                     /gene="murF"
                     /locus_tag="ACHKXP_000749"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO7645818.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27239..28726)
                     /gene="murE"
                     /locus_tag="ACHKXP_000750"
     CDS             complement(27239..28726)
                     /gene="murE"
                     /locus_tag="ACHKXP_000750"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO7645819.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28713..30479)
                     /gene="ftsI"
                     /locus_tag="ACHKXP_000751"
     CDS             complement(28713..30479)
                     /gene="ftsI"
                     /locus_tag="ACHKXP_000751"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO7645820.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30495..30860)
                     /gene="ftsL"
                     /locus_tag="ACHKXP_000752"
     CDS             complement(30495..30860)
                     /gene="ftsL"
                     /locus_tag="ACHKXP_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO7645821.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30857..31798)
                     /gene="rsmH"
                     /locus_tag="ACHKXP_000753"
     CDS             complement(30857..31798)
                     /gene="rsmH"
                     /locus_tag="ACHKXP_000753"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO7645822.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31800..32258)
                     /gene="mraZ"
                     /locus_tag="ACHKXP_000754"
     CDS             complement(31800..32258)
                     /gene="mraZ"
                     /locus_tag="ACHKXP_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO7645823.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32860..33864)
                     /gene="cra"
                     /locus_tag="ACHKXP_000755"
     CDS             complement(32860..33864)
                     /gene="cra"
                     /locus_tag="ACHKXP_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO7645824.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33946..34032)
                     /locus_tag="ACHKXP_000756"
     CDS             complement(33946..34032)
                     /locus_tag="ACHKXP_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO7645825.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34044..34535)
                     /gene="ilvN"
                     /locus_tag="ACHKXP_000757"
     CDS             complement(34044..34535)
                     /gene="ilvN"
                     /locus_tag="ACHKXP_000757"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7645826.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSGKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34538..36262)
                     /gene="ilvI"
                     /locus_tag="ACHKXP_000758"
     CDS             complement(34538..36262)
                     /gene="ilvI"
                     /locus_tag="ACHKXP_000758"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO7645827.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHGLESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36381..36425
                     /locus_tag="ACHKXP_000759"
     CDS             36381..36425
                     /locus_tag="ACHKXP_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645828.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(36580..37524)
                     /gene="leuO"
                     /locus_tag="ACHKXP_000760"
     CDS             complement(36580..37524)
                     /gene="leuO"
                     /locus_tag="ACHKXP_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO7645829.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            38186..38272
                     /gene="leuL"
                     /locus_tag="ACHKXP_000761"
     CDS             38186..38272
                     /gene="leuL"
                     /locus_tag="ACHKXP_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO7645830.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            38365..39936
                     /gene="leuA"
                     /locus_tag="ACHKXP_000762"
     CDS             38365..39936
                     /gene="leuA"
                     /locus_tag="ACHKXP_000762"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO7645831.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITEYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            39936..41027
                     /gene="leuB"
                     /locus_tag="ACHKXP_000763"
     CDS             39936..41027
                     /gene="leuB"
                     /locus_tag="ACHKXP_000763"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO7645832.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            41030..42430
                     /gene="leuC"
                     /locus_tag="ACHKXP_000764"
     CDS             41030..42430
                     /gene="leuC"
                     /locus_tag="ACHKXP_000764"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO7645833.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            42441..43046
                     /gene="leuD"
                     /locus_tag="ACHKXP_000765"
     CDS             42441..43046
                     /gene="leuD"
                     /locus_tag="ACHKXP_000765"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO7645834.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(43095..44273)
                     /gene="setA"
                     /locus_tag="ACHKXP_000766"
     CDS             complement(43095..44273)
                     /gene="setA"
                     /locus_tag="ACHKXP_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO7645835.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLYLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLILFHSRLALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVIALFLTAKVKDV
                     "
     gene            complement(44375..44506)
                     /gene="sgrT"
                     /locus_tag="ACHKXP_000767"
     CDS             complement(44375..44506)
                     /gene="sgrT"
                     /locus_tag="ACHKXP_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO7645836.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            44595..46250
                     /gene="sgrR"
                     /locus_tag="ACHKXP_000768"
     CDS             44595..46250
                     /gene="sgrR"
                     /locus_tag="ACHKXP_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO7645837.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLKIKEIDYDQWHTGEIES
                     DIWLNSANFTLPLDFSVFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLLHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      46284..46383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            46414..47397
                     /gene="thiB"
                     /locus_tag="ACHKXP_000769"
     CDS             46414..47397
                     /gene="thiB"
                     /locus_tag="ACHKXP_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO7645838.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            47373..48983
                     /gene="thiP"
                     /locus_tag="ACHKXP_000770"
     CDS             47373..48983
                     /gene="thiP"
                     /locus_tag="ACHKXP_000770"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO7645839.1"
                     /translation="MATRRQPLIPGWLIPGVSATTLVVAVALAAFLALWWNAPQDDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVIPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLATLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRSWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQVLEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            48967..49665
                     /gene="thiQ"
                     /locus_tag="ACHKXP_000771"
     CDS             48967..49665
                     /gene="thiQ"
                     /locus_tag="ACHKXP_000771"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO7645840.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTMPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAVQQGKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTNELL
                     SGKASASALLGITG"
     gene            complement(49779..50543)
                     /gene="yabI"
                     /locus_tag="ACHKXP_000772"
     CDS             complement(49779..50543)
                     /gene="yabI"
                     /locus_tag="ACHKXP_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7645841.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIIGCLMGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(50629..51507)
                     /gene="araC"
                     /locus_tag="ACHKXP_000773"
     CDS             complement(50629..51507)
                     /gene="araC"
                     /locus_tag="ACHKXP_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO7645842.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            51846..53546
                     /gene="araB"
                     /locus_tag="ACHKXP_000774"
     CDS             51846..53546
                     /gene="araB"
                     /locus_tag="ACHKXP_000774"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO7645843.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKTQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            53557..55059
                     /gene="araA"
                     /locus_tag="ACHKXP_000775"
     CDS             53557..55059
                     /gene="araA"
                     /locus_tag="ACHKXP_000775"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO7645844.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGKKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            55344..56039
                     /gene="araD"
                     /locus_tag="ACHKXP_000776"
     CDS             55344..56039
                     /gene="araD"
                     /locus_tag="ACHKXP_000776"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7645845.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGAKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLNKH
                     YLRKHGAKAYYGQ"
     gene            56114..58465
                     /gene="polB"
                     /locus_tag="ACHKXP_000777"
     CDS             56114..58465
                     /gene="polB"
                     /locus_tag="ACHKXP_000777"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO7645846.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVTSRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            58630..61536
                     /gene="rapA"
                     /locus_tag="ACHKXP_000778"
     CDS             58630..61536
                     /gene="rapA"
                     /locus_tag="ACHKXP_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO7645847.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            61548..62207
                     /gene="rluA"
                     /locus_tag="ACHKXP_000779"
     CDS             61548..62207
                     /gene="rluA"
                     /locus_tag="ACHKXP_000779"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO7645848.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(62324..63139)
                     /gene="djlA"
                     /locus_tag="ACHKXP_000780"
     CDS             complement(62324..63139)
                     /gene="djlA"
                     /locus_tag="ACHKXP_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO7645849.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            63394..65748
                     /gene="lptD"
                     /locus_tag="ACHKXP_000781"
     CDS             63394..65748
                     /gene="lptD"
                     /locus_tag="ACHKXP_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO7645850.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            65801..67087
                     /gene="surA"
                     /locus_tag="ACHKXP_000782"
     CDS             65801..67087
                     /gene="surA"
                     /locus_tag="ACHKXP_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO7645851.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            67087..68076
                     /gene="pdxA"
                     /locus_tag="ACHKXP_000783"
     CDS             67087..68076
                     /gene="pdxA"
                     /locus_tag="ACHKXP_000783"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO7645852.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            68073..68894
                     /gene="rsmA"
                     /locus_tag="ACHKXP_000784"
     CDS             68073..68894
                     /gene="rsmA"
                     /locus_tag="ACHKXP_000784"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO7645853.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            68897..69274
                     /gene="apaG"
                     /locus_tag="ACHKXP_000785"
     CDS             68897..69274
                     /gene="apaG"
                     /locus_tag="ACHKXP_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO7645854.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            69281..70123
                     /gene="apaH"
                     /locus_tag="ACHKXP_000786"
     CDS             69281..70123
                     /gene="apaH"
                     /locus_tag="ACHKXP_000786"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO7645855.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(70201..70680)
                     /gene="folA"
                     /locus_tag="ACHKXP_000787"
     CDS             complement(70201..70680)
                     /gene="folA"
                     /locus_tag="ACHKXP_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO7645856.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(70872..72734)
                     /gene="kefC"
                     /locus_tag="ACHKXP_000788"
     CDS             complement(70872..72734)
                     /gene="kefC"
                     /locus_tag="ACHKXP_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO7645857.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(72727..73257)
                     /gene="kefF"
                     /locus_tag="ACHKXP_000789"
     CDS             complement(72727..73257)
                     /gene="kefF"
                     /locus_tag="ACHKXP_000789"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO7645858.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(73365..74696)
                     /gene="yaaU"
                     /locus_tag="ACHKXP_000790"
     CDS             complement(73365..74696)
                     /gene="yaaU"
                     /locus_tag="ACHKXP_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7645859.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(74755..75042)
                     /gene="fixX"
                     /locus_tag="ACHKXP_000791"
     CDS             complement(74755..75042)
                     /gene="fixX"
                     /locus_tag="ACHKXP_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO7645860.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(75039..76325)
                     /gene="fixC"
                     /locus_tag="ACHKXP_000792"
     CDS             complement(75039..76325)
                     /gene="fixC"
                     /locus_tag="ACHKXP_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7645861.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(76376..77317)
                     /gene="fixB"
                     /locus_tag="ACHKXP_000793"
     CDS             complement(76376..77317)
                     /gene="fixB"
                     /locus_tag="ACHKXP_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7645862.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(77332..78102)
                     /gene="fixA"
                     /locus_tag="ACHKXP_000794"
     CDS             complement(77332..78102)
                     /gene="fixA"
                     /locus_tag="ACHKXP_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO7645863.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        18898 a        21650 c        19786 g        17834 t
ORIGIN      
        1 gattttttaa cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt
       61 tgtgattttt aattcaatta aaacgaattt aaattcattc tgcatattga gaggggttga
      121 ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg
      181 gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc
      241 aaacttcttt gatgtaaaca aattaataca acaaacggaa ttgcaaactt acacacgcat
      301 cactgcgtag atcaaaaaaa caaccaccgc acgaggtttc atgatggaag gtcaacagca
      361 cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg
      421 cgcgataggg accgggttat tcctgggtag cgcctccgta atacagtccg cagggccagg
      481 gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg
      541 tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg
      601 gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc
      661 catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac
      721 ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa
      781 agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg tagtagcgat
      841 gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag
      901 caacctgtgg gatcagggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat
      961 ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc
     1021 tgataacccg gagcaaagta taccgaaagc aactaaccag gttatctacc gcatcctgat
     1081 tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc
     1141 cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc
     1201 gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa
     1261 cagccgtatg ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt
     1321 cgataaacgt ggtgtaccag taaataccat tctggtgtct gcactggtaa cggcgttgtg
     1381 cgtactgatt aactaccttg ccccagagtc cgctttcgga ctgttaatgg cgctggtggt
     1441 atctgcactg gtaatcaact gggcgatgat tagcctggcg catatgaaat tccgtcgcgc
     1501 caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg
     1561 gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc
     1621 gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa
     1681 agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca cccgtacagg
     1741 gtgagggcaa taatctttac accaacgccc cgtaaatcgt cagtagtgca atcaccacca
     1801 cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat
     1861 cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg
     1921 aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca ccatgaccga
     1981 tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg
     2041 catcaatgcc ggactgtaaa cgatgatgcg gcgtctgata tcgcgccagc cagtattgcc
     2101 atgcacgcaa aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca
     2161 gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct
     2221 cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc
     2281 caaccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat
     2341 aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagta
     2401 gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca
     2461 tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga
     2521 acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa
     2581 ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa
     2641 ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg
     2701 gcccgtcatg ttattagcga tatccggata gcaatcaatc agtgcccgcg taaccgccgc
     2761 aagctgttga tactgcgcat cggtatacgc cagcgtatcg gtgccttcaa gctcaatccc
     2821 aatagaaaaa tcattgcagc gttcgcgccc ctgatactga gagactcccg catgccatgc
     2881 acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc
     2941 ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc
     3001 agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg
     3061 caccaccagc agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac
     3121 gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg
     3181 tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt
     3241 tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct gctggaacgc
     3301 attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca
     3361 gtcgatgcca acaatgatat tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc
     3421 acggtgatca cccgcgagaa tggcgtcttt tgcggcaaac gctgggttga agaggtgttt
     3481 attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc
     3541 aataccaatc aatccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa
     3601 cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat
     3661 gtcgaattgc tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacccggc
     3721 ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg
     3781 ctttctgatg ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag
     3841 gccgtcgaaa aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat
     3901 ctggaagaac ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc
     3961 gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg
     4021 tctggcaacg tcactgacaa aacactgcgt gaatttgccg aaacgggcgt ggactttatc
     4081 tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa
     4141 tagttgacag atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt
     4201 cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg
     4261 ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa agtagcgcca
     4321 accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat
     4381 ggtggttatt ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta
     4441 cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc gtaccgccgt
     4501 agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat
     4561 tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc
     4621 gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacaccgg gttgggataa
     4681 cgcaaacggc gtcaccggct ggacgcgcaa ctgcaatatt caaagtgaca gcgcattgca
     4741 gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat
     4801 tccacagctc actgccctgt gtctgcgtta tcatggagtc ttgctggatg ccagcgaaga
     4861 ggtggttcat gttgcggtag tcgatgcacc ttcgcatgag ctactggacg cattgcattt
     4921 cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggtcacgc
     4981 cagtcgcaca caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga
     5041 gttgctaact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata ttcatatcga
     5101 accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc
     5161 ggatgtttca ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct
     5221 ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa
     5281 cgccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag
     5341 gttgttacag caggtgggtc aggcactgga tgtcaacacg cttggaatac agccgttaca
     5401 actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc
     5461 taccggcagc ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga
     5521 cattaatatt tgtagcgtcg aagatccagt tgagatcccc atagccggac taaaccagac
     5581 gcaaatccat ccgcgtgccg gactcacctt tcagggcgtt ttgcgtgcgt tattgcgcca
     5641 ggatcctgac gtcatcatga tcggagagat ccgcgatggc gaaacagcag agatcgctat
     5701 taaagcggcg caaactggtc acctggtgtt gtctacccta cacactaatt ccacctgcga
     5761 aacgctggta cgtttacagc aaatgggggt cgcccgctgg atgctatcat cggcgcttac
     5821 gctggtaata gcccagcgtc tggtacgcaa actttgccca cattgtcgcc ggcagcaagg
     5881 ggagcccatc cacattccag acaatgtatg gccatcgccg ctgccccact ggcaggcacc
     5941 cggttgtgta cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc
     6001 cataacgccg gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac
     6061 gcacgcacga caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtagagca
     6121 aggcttaacc acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc
     6181 aactctggcg ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg
     6241 cagagagccg tactttactg cttatggcac tacagcaaca gatggttacc ccactaagcc
     6301 tgaagcgaat cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc
     6361 atcaactggc gacgctactc aaagcagggt taacgctttc tgaagggctg gctctgctgg
     6421 cggaacagca tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg
     6481 aacagggcat tgctttttcc aatgccttat taccctggtc agaggtattt ccgccgctct
     6541 atcaggcgat gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac
     6601 tggcgcgtca gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt
     6661 atcccatcat cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc
     6721 tgccggagtt tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg
     6781 ggatcatgac gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct
     6841 ttctgctggc gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc
     6901 agaaattgct gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc
     6961 agatctttac gattctggcg ctgacacaaa gtgcaggcat tactttttta cagggcgtag
     7021 agagcgtcag agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc
     7081 acgatatcag taacggtcaa cccatctggc tggcgctaaa aaataccggt gagtttagcc
     7141 cgctctgttt gcaattagtg agaacaggag aggcatccgg ctctctggat ctcatgttag
     7201 acaacctcgc ccatcatcat cgggaaaaca caatggcgct ggcggataac ctcgcagcct
     7261 tactggaagc ggcgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcaa
     7321 tgtatctgcc aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg
     7381 ttgctccacg ccagcgttgg gagattacag gttgttgaag atgcggtttt cttgttccag
     7441 cacacgaata aacgtggtgc gcttggtcag ctctttcagg cgtgaagccc caacgtatgt
     7501 acaagctgaa cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag
     7561 cggcagctta acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg
     7621 tttcatcgca gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc
     7681 aacgatgcga ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc
     7741 ggcaccgccg ccaaaagctt tcgcgacatc gcccggcgtg gtgcagccac catcgctgac
     7801 gatcattccg cccagaccgt gcgcagcatc ggcacattcg attaccgcag aaagttgcgg
     7861 ataaccgacg cctgttttga cacgagttgt acaaacagaa cctgggccaa tgccaacttt
     7921 aacgatatcg gcaccggaga ggataagctc ctcacacatt tcaccagtta ctacgttgcc
     7981 agcacaaatg gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg
     8041 ttcggaataa ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag
     8101 aatctgttta gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt
     8161 cagcacatca gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt
     8221 atgcacagca gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtgcctac
     8281 ggtgtccata tttgcagcga taatcggcac gccggaccag ctctgacctg aatgtttgaa
     8341 ggtgaattga cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg
     8401 gatgagaacg tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct
     8461 ggggttaatg gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc
     8521 aatgcggcgc aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg
     8581 gtaaatagtg agcgaacgtt tcatcaattt cgctttcgtg cgctcatttt taacgttttg
     8641 ataatcatga tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag
     8701 gcattggcag tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca
     8761 ttgatgccga tattatcgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca
     8821 ttgctgatca ctttggcgct aacatgattg ctgcggatgg aacattgcag cgccgggcct
     8881 tgcgcgagcg gatcttcgcc aacccggaag agaaaaactg gcttaacgac ctgctgcatc
     8941 cgctgattca gcaagagacg caacaccaga tccagcaagc aacctccccc tatgtactgt
     9001 gggttgtgcc attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg
     9061 tggatgtcag cccagaaacg cagcttaagc gtaccatgca gcgcgatgat gtaactcgcg
     9121 agcatgtcga acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag
     9181 atgacgtcat tgataataac ggcgcaccgg atgctatcgc atcggatgtt gctcgcctgc
     9241 acgcacacta tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac
     9301 ccaggtcctt tttgaacatc cactaaacga aaaaatgcgt acatggctgc gcattgagtt
     9361 tttgattcag caactcaccg ttaatttacc catcgctgac cacgctggcg cgctgcattt
     9421 cttccgtaat gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct
     9481 gttgaaagaa cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt
     9541 ggaccagagc cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat
     9601 ctccgcgccg cgtatcgggc aatttctacg tgaagatcgt ttgattgctc tggtgcgtca
     9661 gcgactgagc atcccaggcg gctgttgcag ctttgattta ccaacattgc acatttggct
     9721 gcatctaccc caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc
     9781 gctcacccag gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa
     9841 acaaaccagc ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct
     9901 gaatctgtcg ctcgattcac agctgtatcc gcaaatttcc ggtcataaga gccgttttgc
     9961 cattcgtttt atgccgctgg acagtgaaaa cggacaggta ccggaacgtc tggatttcga
    10021 actggcctgt tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg
    10081 cgggaaaacg gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg
    10141 tcagctgatc gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga
    10201 tctttccgaa agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc
    10261 acgtaggtct gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg
    10321 cggcgtgaac gccttatccg acctacaaaa acaaatttat caatgagtta gaaatccctg
    10381 taggtctgat aagacgcgcc agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc
    10441 gtgaacgcct tatctgacct acagacgttt aagcttcgca attaccggtt cattggctgg
    10501 cggaaaatca tcggcattaa gaccgaccag cgacatccac ttaccgggtt gcccttcttt
    10561 accccacggc tccccttccc agctttcgac cagccagaac cacagagtta tatgcctgtc
    10621 cgggaactca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa tcccgacttc
    10681 ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc
    10741 gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgggtta taaagatttc
    10801 attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg tcttaaacct
    10861 ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta ttgcaggcga
    10921 ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt acgtcctact
    10981 ttgcgttcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc atcctgatgg
    11041 ctaagctgct gcatttgcgc taaacgctcg gcttccatac gacgctgttg ttccagctcc
    11101 tcaacctctt caggcatacg tacctgaact ttgctcagcg tactgataac ttcatatttc
    11161 aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc ctgcttcgga
    11221 tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat cgctgccagg
    11281 tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcaaa gtgacgcatc
    11341 atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg cgccagaatg
    11401 cgctcacgca gcgtctcttc atgcagttcg ggttctttat ccagccactc agcaattggt
    11461 aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc ccacatttct
    11521 tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc ttcacgaatg
    11581 ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg ggagtaaatg
    11641 gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg aatgtcgaag
    11701 ttacggcttt caactttacg ctgggcgttg gcaatcgctt tggtcaccca cgggtgttca
    11761 atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg gtcggaagca
    11821 aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc agcatccccc
    11881 tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg acgctcggta
    11941 ccgatgatat gcaggccacc tgctgccagt accgcatcgt gacgtacctg ccagtcggct
    12001 ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc tgcctgccag
    12061 ctaccaccga gcacaatatc tgtaccacgc cccgccatgt tggtcgcgat agtcaccgca
    12121 gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt ggcgttcaga
    12181 acgttgtgct tgataccggc tttggtcagt tcgtttgaca ccagctccga tttttcaatg
    12241 gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat atcttcaatg
    12301 atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc tttacgaatc
    12361 attggacggt tggtcggaac aacgacggta tccagtttgt agatggagct aaattcgaaa
    12421 gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg gaagtagttc
    12481 tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc ttctttcgct
    12541 tccacagcct ggtgcagacc atcggaccag cgacggccct gcatggtacg accggtgtgt
    12601 tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcacg ggtaaacagc
    12661 gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc cggagagtac
    12721 agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat cagcaccaga
    12781 ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc
    12841 tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat tttattcacg
    12901 cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct
    12961 tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt acgctgtaca
    13021 cgttcttcag ggctgaacgc catgttgtcg cgcaggtagt caaagccgta ttcgttgttc
    13081 gtaccgtaag tgatgtcagc tgcataagct tcacgctttg ccggtgctgg catgcccggc
    13141 aggttgatac caacagtcag gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca
    13201 cgttgcgcca ggtagtcgtt gacggtaact acatgcacgc ctttaccggt tagtgcgttc
    13261 aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg
    13321 cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg cataccaaag
    13381 acgcgcttac ttgcctcacg taccacggcg aaagcttccg ggatcagatt ttccagcact
    13441 tcgccttttt ccagacgcgc acgaaactct gcggttttcc ctttcagttc ttcgtcggag
    13501 agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg catccggcgc
    13561 agggtgcgat cgttacgact accgaaaact ttagttaata atttgattag cataataaaa
    13621 tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt gttgttagca
    13681 gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg gcgtgctgaa
    13741 ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag acggcgtgcc
    13801 ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg cctgaagagg
    13861 caaagattct tcagcaacgg gcagcgtttg cggtgccatt gcgaaagaaa gatgacggat
    13921 taccgtgcga atagcatgtt gatgccagta atcaacggaa tagttcgaat tcgggcggcg
    13981 cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag gctcgtggtt
    14041 gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc
    14101 taaactcgcc gcaaccatcc ctaataagag atgcggccag aagtagcgtt taccaaactg
    14161 tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgtatt caaacgtatt
    14221 tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag ataagttgtg
    14281 cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta taaaattcaa
    14341 aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc gaattttata
    14401 cgacagtata aatgtcgtta tgccagtacg gctgaaggcg ctttgaaggc caacggcagt
    14461 tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccag gacagcctgc
    14521 agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc aataatattg
    14581 tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac aaattcgtct
    14641 tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat ggcacaatcg
    14701 aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg catgaaaccg
    14761 aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt catcgcatag
    14821 cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa
    14881 ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac agtctctttg
    14941 atgcgaacaa atttcttggc gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat
    15001 acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac ttcgataaca
    15061 atgttatcga tgcccaggcc cgcgagagca gcattgaggt gctctacggt tgaaatccgt
    15121 acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga tttggcatcg
    15181 gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc ggtgttggcc
    15241 ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc
    15301 tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca aattacctat
    15361 ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc tcgaaaccca
    15421 aattccagtc aattcttaat cagcttgctt acgcaggaat gctgggatat ccagataatc
    15481 cggctctttc gcagtttgcg gcgcattgtc attcacgact ttagcaaccg gcttctgctc
    15541 ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct gctgaacctg
    15601 cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg tcgcaacaac
    15661 ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc
    15721 gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat ccagacgcag
    15781 gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt cgatatcttc
    15841 cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt caccgctcgc
    15901 cacgccagaa cccatcattg cgtagcccat ctcagacatt acggtgcgta cgtctgcaaa
    15961 gtccacgttc atcaaacccg gacgagtaat cagttcagcg ataccttgca cagcgccttt
    16021 cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc ccagaacttt
    16081 cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca gttcagtgat
    16141 cccctgctcc gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct tagtgacgac
    16201 agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg ctgcacctgt
    16261 accggtacca ccacccatac ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc
    16321 acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat tagcgccagc
    16381 gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg
    16441 cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatgc gctcgcgcac
    16501 catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc
    16561 attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg tcgcctgagg
    16621 ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac tattgagtcg
    16681 cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt caccgttaag
    16741 atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg gctcctgagc
    16801 ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac
    16861 gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc
    16921 tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact gcaatatctc
    16981 ttcgttgacc aggttgagca gctcggtata gcgcggctcg atcacctctg ccagtgtctg
    17041 acgttgcaga ctccgtggcg gacgaccacc tacgctcggc acttccacgc tctcatcttt
    17101 tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg
    17161 cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat aaggaattac
    17221 cttagtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac caccaccgat
    17281 atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat aacttgatgc
    17341 cagtccggca aatatcagtt ggtcaacttt cagcccacaa cgttcaaccg ctttgacgat
    17401 gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca tccgcacgcc
    17461 cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact cttgcgggat
    17521 cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt
    17581 ttccacatct tcttgcgtta cttcttcttc agaaataggc accataccaa tttcattctg
    17641 gcagctgata tgcttaccag aaagcgccag atataccgaa gagatctgac aatctgccat
    17701 caattctgcc tggtcaatgg cgcgctgtac gcacttgacc acggattcga ggtcgttcac
    17761 cccgccttta tccataccac gcgacgggca gctgcccacg ccaatgatat tgaccatacc
    17821 gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaatct ccagtcctac
    17881 taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg attttgttgc
    17941 tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga gtcataacgc
    18001 aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa aaccggataa
    18061 agttctacaa agcgagccaa acgtttcatc gtatcgcccc ggccaagatt gagcttaata
    18121 tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta
    18181 aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac ttcattagcg
    18241 ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc tggcggcacg
    18301 ctgaaggtgt ttccttccgc gtctaccatg tgttgatcat tccaccgcgc aatcggcaca
    18361 tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct cacctgctta
    18421 atccacggca ggcgttgttc tatttgcgtc tggatgatgt tgacatcctg ggtcataaag
    18481 gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg cgtgtaatgg
    18541 cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc catccagccc
    18601 aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa aaggatcccc
    18661 gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcgctatt tcgcgtgttc
    18721 agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca actgcgagaa
    18781 gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca tacccggtga
    18841 ggtattggct tccagcagat aaaactgtcc atcgctgtcc agcataacgt caatacgtcc
    18901 ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca aattggcctc
    18961 ttgtgacgct tccagacctg cggggcagaa atactgtgtc tcatcagaga gatacttcgc
    19021 ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa tttcttcacc
    19081 gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata cttcttcatc
    19141 gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta cttttgacat
    19201 tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca gagcagaaat
    19261 ttctgctaac tgcttatcgc tcaggccttt ttcaaactct gcgcgggtta acgctaccca
    19321 cggcgcgacc ggtaaaccgg caccttgcca cagcagtttg ctgcgtagtt tatccattga
    19381 aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct cgagcatgcc
    19441 ctgcagcgta ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt tctgaaaacc
    19501 catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg catcaatacc
    19561 gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt cccgctcagc
    19621 ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc tccggagttt
    19681 gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta ccagccccct
    19741 gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct acctgcgcag
    19801 ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga cacagcgaac
    19861 ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc atcaacaggg
    19921 tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac
    19981 ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc gccgctttaa
    20041 tggtggcgtc cacttccgtc gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc
    20101 ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg cgaccagtcc
    20161 cctggaagct ttcaagcgcc cgcaaaatag cctcgtcgtc aatgccctct tccgtagcaa
    20221 ccgcaaccgc agctgcggcg ttcagcgcgt tatgacgacc tggcgcattc agggtgacgc
    20281 gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc ctgcgggcca atctgctgat
    20341 aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agtcgtggtc tgacgcccca
    20401 ctcgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca cgaccgtaaa
    20461 acggcaggtt gtgcagaaaa ttaataaaag tctgttttaa attctcaaag tcgccctggt
    20521 aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt tgcagatgca
    20581 ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg
    20641 catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg agccccgctt
    20701 ctgcgtagat gctggaaacc atcgcggtgg ttgtcgtttt gccgtgcgtt ccggcaatgg
    20761 cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg atcaccggaa
    20821 tacgcgcttc atgagcggcg acaatttccg ggttatcggc agaaatcgcg ctggaaacaa
    20881 cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc gtcgcaccca
    20941 gattcattaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg atctgataac
    21001 cttcattggc cagaacttcg gcaataccgc ccataccggc accaccaatg ccgacaaaat
    21061 gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc aattgttgtg
    21121 tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc atcgctacaa
    21181 ttacgcccgg gcaacccggc tcacttcatt tgccactcgc tcggtggcat ccggaatgga
    21241 tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc cggccagggt
    21301 gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg cgcccgcttt
    21361 ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg gcacaaacaa
    21421 cgccggtagt cctgccgcgg cgatttcact caccgttaac gcaccggagc ggcaaacgac
    21481 gacatccgcc cacgcatacg ccgccgccat atcatcaata aattccgtca ctttatgctg
    21541 cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt tgccgctctg
    21601 atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg tctggttaag
    21661 aatgcgtgcg ccctgagaac cacccactac cagcacacga accggacctt cacgtccagc
    21721 caaacgttgc tgcggcaacg gcagcgccaa cacatcggta cgcaccgggt tacccactac
    21781 ttccgcatta gggaaagcac ctggaaacgc ctgcatcact ttggtggcaa tcttcgccag
    21841 ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg gaatgcctaa
    21901 cgaccacgcg gccagaccac ctggacctga cacgtagcct cccataccga gcaccacgtc
    21961 aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg
    22021 ggcagctatc agtgctttta taccttttcc acgcagacca gagatacgaa tgaaatcaat
    22081 ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg
    22141 aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc agtcccggga atacatgtcc
    22201 accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg tgaacctcgt
    22261 acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag catcatgatg
    22321 gctgtcgaca taatcagtaa gctcgaacca ccgtaactga tcagcggcaa tgtcagacct
    22381 ttggtcggta acatccccgc cgccgcgcct acgttaacca gcgcctggaa gctaaaccag
    22441 atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa tgctttacgg
    22501 ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta aaagtgccag caccacaccg
    22561 acatacccca gttcttcgcc gataatggcg aaaataaagt cagtgtgcgc ttccggcaga
    22621 tactccagtt tttgtaccga gttacctaaa ccttgccccc aaagttcgcc gcgaccaaac
    22681 gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca cgggttccag
    22741 aatgcggtaa cacggcggat acggtacggt tcggcgagta tcagcaacac aaccgctgaa
    22801 atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag gaacaacatc
    22861 gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc cagcagtaac
    22921 actgccaaca ccagaatcac gcccatcggt ttcaggaagc cgcgcaggtt attacgtact
    22981 tcgtcgcctt tacgcaccag atagttggcg atatagcaaa acagcgacag ttttgtcagc
    23041 tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc tttaaccgag
    23101 ctacccacta ccaggacgat catcagcagg atgatagatc cgagcagcat cgtggcactg
    23161 tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat aaacgccaaa
    23221 atcagataga caccatcacg cttcgcgaag aagaacggat cgttggttaa gcgttgccct
    23281 atgggcattg acgccgaggt caccatgata aagccaatcg ccgcgaggcc gaaggtcagc
    23341 cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg cgagcccatc
    23401 acccagccct ttagcgccgt ggagatccag accaggatac tgaatcctgg caggcgcggc
    23461 attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga cgggcaaact
    23521 cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggct ggggagagca
    23581 gaaccatatc gcccggctga acacgcggag ccagcaagcg catcgcctgt tccatagttt
    23641 cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcgccgtca cgaccgaaac
    23701 aatacagacg tacgttatcg ccattcaggt aacgcgccag tgggctaaag tccgccgatt
    23761 taccatcgcc acccagcaac aaatgcagtg tgccgtctac gtgcaggcca ttcagcgccg
    23821 cttccgtact gccgacgttg gtcgctttcg aatcgttaat ccaacgtacg ccgttatgct
    23881 ccagcacaac ttcaaagcga tgcggcagac cagtgaatgt ggttaacgct ttcaggctgc
    23941 tggcacgcgg taaccctgca gcatctgcca gcgccagcgc cgccagcgca ttggtgtagt
    24001 tatgctgccc ggaaagtttc atctctttca cattcagcac tttctcgcct ttaacccgca
    24061 gccaggtttc gccctgctga tgattcaggt gatagtcacc catgttgacg ccaaagctga
    24121 cgcagcgttc atccgcaccg cgaatcggca ttgttaaggc atcatcagca ttaaccacgc
    24181 aaactttcgc gttttcgtaa atgcgcagtt ttgctgcacg atactgttgt aaaccaaacg
    24241 gatagcgatc catatgatct tcagtcacgt tcagaatggt cgctgctacc gcctgtaagc
    24301 tggaggtggt ttccagctgg aagctcgaca gttccagcac gtacagttca cactcatcat
    24361 ccagtagcat caacgcaggc aggccaatat tgccacccac accaacgtta acccccgccg
    24421 ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg
    24481 ccacaatcgg tgcttgtgct tcgcgacaga acagctcgat atcgccaacg atttcgattc
    24541 cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga ctggcgacaa
    24601 tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt tctacggctt
    24661 cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac
    24721 cgcgagcgag gaaaaagtcc acgcaggaaa gcccggtgag gcccaggccg ataatgacga
    24781 catttttacc ctgataatca gccatgatta acgtaccttc agcgttgcca gaccaatcag
    24841 aaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg gccagccttt
    24901 cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa
    24961 ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc ccataatcac
    25021 cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac ctaacgccag
    25081 cgaacctaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca ggaagcccag
    25141 tcctgccccg actatcgcgg tacagacaat aaccagttcc ccggcgtgtc gcagatacgg
    25201 tatatgcaag tagctggcaa agttcatatt gccggtcgcc cacgccacca gcgcaaaacc
    25261 accggcgaca aatacggtcg gcataattgc caggccgtcg agaccatcgg tcaggtttac
    25321 cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca gccccagctg
    25381 cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc
    25441 aaggtacagg gcgaaggcga cacccagcgc aatgaccgac atccagaaat acttccaacg
    25501 agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa caaagccaat
    25561 aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg acgggtaagc
    25621 ccacagcagt acggagatca caatcgccgt caggatcata atcccgccca tggtcggcgt
    25681 accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc accacctgac caaaggaaag
    25741 tttttgcaaa tgagcaatca tacgcgggcc catccacaat gagatgaaca gcgcggtcag
    25801 caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg aataatattt
    25861 gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaaagc gcgtactacc
    25921 tcttccatgg cggcactacg tgaaccctta actaaaatcg taattacctg ttgctcagca
    25981 atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc gccaacgccg
    26041 ctggcggtgc tgatagcatg gctttgttta cccacgctta acacgcggtc aataccagca
    26101 gcttttgccg cctcgcccac ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc
    26161 atatcgccca ccacaagcac gcggtagccc ggcatttcag ccagtacctg gactgctgca
    26221 gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt ttctgccagt
    26281 tggatgggga acagacggcc tggaacagct ttcagatttg ccagccccgc tttgatagca
    26341 tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt cgcaatattg
    26401 tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg tagggtaaat
    26461 tccgtaccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt ggcggcattg
    26521 ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag ccagtcgttg
    26581 ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat ttcacctttc
    26641 gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc caggttgttg
    26701 accagcgcag cttccgggcg agtcagactc acagtccagg ctatttcgcc ctgatggttc
    26761 gcgccaagtt caataactgc gtaatcgtat tccggcgtta agcgcaacag cgtcatcggt
    26821 acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca ctggcttaaa
    26881 atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag agcaaccacg
    26941 cgcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag acgcgtatcc
    27001 ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg
    27061 ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcgcc tttcagggca
    27121 acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc aagggtgata
    27181 tctgcacctt gcagttcacc gttgagaatg tcggtaagtt ggctaagggt tacgctaatc
    27241 atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat
    27301 tgccaacaat ctggtaatct tcatggcctt tgcccgcgac cagtaccaca tcattctctt
    27361 tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact ttggcatgtc
    27421 cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct tcggtacgcg
    27481 ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg cccatcagtg
    27541 gacgcttacc tttatcgcga tcgccaccac agccaaagac acaccacagc ttgcccgcac
    27601 agtgcagacg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca
    27661 ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa accggttgca
    27721 gacgcgcggc ggttttcagc agatcagcca gtggatagcc gagtgccaac agtgtcgcca
    27781 gcgcgagcag caggttgctg acgttaaaag cgcccatcag atggctttca atttcgccat
    27841 cgccccaact tgagctaaag cgaatcgtcg caccgctgtc gtgatagttc acttcggtcg
    27901 ctttcaacca gcgtccgtga cagttcggat taatatgatc ttccattgat accgcaaccg
    27961 cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta ataatcgcct
    28021 gaccgcaatg atgctcagaa taaagcagcc atttcgcggc ttcgtagtgt tccatatcac
    28081 catgataatc aagatgatcg cggcttaagt tggtaaagac cgacgccgca aatttcaatg
    28141 ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa aacgtcgcgc
    28201 cctgatccac cagccccgcc agctcatgct gaacatcgac tgccgaaccg gttgtatttt
    28261 ctgtcgggat cactttcccc agcaggccgt taccaacggt gcccattacc gcgctggttt
    28321 cgccaagcag ttggctccac tgcgccaaca gctgggtagt cgtggttttg ccgttggtgc
    28381 ccgttacgcc cacgagacgt aaattgtcag agggttcatg gtaaaagcgg cccgccagtg
    28441 cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc atttcacgga
    28501 tttcaccatc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    28561 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    28621 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    28681 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    28741 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    28801 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    28861 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    28921 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    28981 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    29041 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggattcc
    29101 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    29161 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    29221 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    29281 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    29341 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    29401 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    29461 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    29521 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    29581 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    29641 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    29701 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    29761 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    29821 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    29881 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    29941 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    30001 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    30061 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    30121 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    30181 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    30241 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    30301 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    30361 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    30421 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    30481 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    30541 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    30601 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    30661 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    30721 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    30781 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    30841 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    30901 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    30961 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    31021 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    31081 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    31141 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    31201 gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc agttctttgg
    31261 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    31321 aacgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct gcggtttgta
    31381 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    31441 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    31501 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    31561 aaaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    31621 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    31681 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    31741 tgaggccatt aacggcttca tccaacagca ccgtagtatg tttatagttt tccatcattt
    31801 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    31861 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    31921 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    31981 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    32041 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    32101 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    32161 tgaccggcag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    32221 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    32281 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    32341 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    32401 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    32461 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    32521 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    32581 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    32641 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    32701 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    32761 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacgcgc
    32821 cagagggaaa ttcacctggc gcgtattttt gttcgcggct tagctacggc tgagcacgcc
    32881 gcggcgatag agattacgtt taatgcgcgt taaaccaggt tttggcttac gcggttcgtc
    32941 caggcttgcc aggacaatct ccagcacacg ctctgcgaca tcgcggtgac gttgagccac
    33001 tgccagcacc ggacactgta agaagtcgag cagttcgtta tcgccaaagg tggcaattgc
    33061 caggtcagaa ggcagtttgc cgtcgcgacg cagcgtgaca tccatcactc cttgcaacaa
    33121 cgcaaacgac gttgtgaaca gcgcctgcgg catcggatgc gtttccagcc atttttcgaa
    33181 taactgggca gccgcctccc gctcatagct gttggcatac aggaaatgca cttcgcgcgg
    33241 atcatctttc caggcagtac ggaaaccttg ttcacgcagg aagctgacag aaagctccgg
    33301 tagcgcacca agataaagca ccgtctcggc gggaaactta cgtaactctt ccgccagcat
    33361 ttcggcatca tcctgatcgg caccaaccac gctggtgaag tgttcacgat cgagggcgcg
    33421 gtccagcgcg acaatcggga acgggtcgtt agcccagcgt tgataaaaag gatgctcagg
    33481 aggcaacgac gtcgaaacaa taatggcatc aacctgacgc tgtaaaaggt gctcaatgca
    33541 ccgcatttcg ttgtctggct gatcttctga gcaggcaatc agcagttgat aaccccgttg
    33601 ccgcgcctgg cgttcaagat agttagcgat gcgggtatag ctggtgttct ccagatcggg
    33661 gatcacaaga ccaatagaac gtgtgcgtcc agcacgaagc ccagctgcca cggcgttcgg
    33721 gtggtaattg tgctcacgca ccacagccat gactttttca acggttttgt cgctcacacg
    33781 gtattgcttc gctttgccgt taataacata gcttgcagtg gtccgcgaca ctcccgccag
    33841 ccgagcgatt tcatccagtt tcacaattgc cccttgcgta aaatgtaaaa accatagcca
    33901 ttgtactggt atgggttaaa ttcattaaca tctaagcgca gaaaattaac tgcggcaact
    33961 gcttttattc caggttccca ctgatttcgc aaaaaaagcc caacgtgaca tgttgggctg
    34021 taaattgcgc attgagatca ttctcaacgc attattttat cgccgcgcga aagtccgacc
    34081 acaccagagc gagcaacctc cacaattttc gccacatcgc gaatcgatgc taaaaatgca
    34141 tcaagcttac cgctggtgcc tgctaattga acggtataaa gcgagggtgt gacatcgata
    34201 atttgcccac ggaatatttc cgtattacgt ttcacttcgt cacgcccgta accgctggcc
    34261 tgaattttca ccagcatgat ttcccgctca acatgcgcgc cctgccccaa ctcactcacg
    34321 cgcaagacat cgaccagttt gtgtaattgc ttttcgatct gctcaagtac tttttcatcg
    34381 cccacggtct ggatggtcat acgcgataat gtcggatcgt cggttggcgc aacggtcagg
    34441 ctttcaatgt tgtagccacg ctgggaaaaa aggccaatca cgcgggataa cgcgcctgat
    34501 tcattttcga gtaagactga taatatccgg cgcataatca ggttctctcc gttttgctta
    34561 accacatttc atccattccg cccccgcgaa tctgcatcgg gtagacgtgc tcgctgccat
    34621 cgacggtaac atcaacaaac accaggcgat tattgcgcac ctgttccagc gcctcgctaa
    34681 gtttgctttc cagcccatgc ggatgagaaa tctggatccc gacatgccca taggcttccg
    34741 ccagacggac gaaatcgggt agcgattgca tataagattg tgaatgacgg ccggaataga
    34801 tcatgtcctg ccactgcttc accatcccca gatagcggtt attgagattc accaccagta
    34861 cgggcaactc gtattgcaac gcggtagaca gttcctggat gttcatctga atactgccgt
    34921 cgccagtgac gcaaaccacg gtttcttctg gcaacgccat tttgacgccc agtgccgcag
    34981 gtaaaccaaa acccatcgtg ccgaggccac cggaattgat ccagcgacgc ggtttgtcga
    35041 atggataata aagtgcagca aacatctggt gctgcccgac atcggacgtc acgtaagcgt
    35101 ctcccttcgt caaccgccaa agagtctcga tcaccgcctg cggtttaatc ttttcactgt
    35161 gagtgtcata tttcaggcac tgacgagcgc gccactgttc aatttgctgc caccagtcgc
    35221 ggatctcatc cagtggttga tgggcggatt cttgcgacaa gagttcaagc atttgttcga
    35281 ggacctggcg agcatccccc acaatcggga tatccgcagt cacggtttta gaaatggaag
    35341 taggatcaat atcgatatgc agaacagtgg catttgggca gtactttgcc agattgttcg
    35401 tcgttcggtc atcaaatcgt accccgacgg cgaaaatcac atccgcgtta tgcatcgtca
    35461 tattggcttc gtaggtaccg tgcattccca gcatgcccag tgcctgacga tgcgttgccg
    35521 gaaacgcccc cagccccatc aatgagcaaa caacgggcag attcaacgcc tccaccgttt
    35581 ctttcaactg ctgatggcag cccgccgtga ttgccccacc gcctacgtag acaaccggtt
    35641 tttttgccgc taccagcgtt tgcagagcac gcttaatttg ccctttatgt ccggtagtag
    35701 tgggattgta agaacgcata ctgaccgact ccggccagac atagggtaat ttgttcgccg
    35761 gattaagaat atctttcggt aaatcaacga ctactggtcc tgggcgacca cttgccgcca
    35821 gccagaaagc ctttttcagc acctgcggaa tgtcttccgt ttgcttaacc agaaaactgt
    35881 gtttaaccac cggtcgcgaa atccccacca tgtcgcactc ctgaaaggca tcgtaaccta
    35941 tcaacgaggt cgctacctgc ccggaaagga caactaatgg aatggaatcc atataagcgg
    36001 tggcgatgcc agtaatcgca ttggtcgccc ctggacccga cgttaccagc acgacgccga
    36061 cttccccggt cgcgcgcgcc aggccatcgg ccatatgcac cgccgcctgc tcatgacgaa
    36121 ctaatacatg atcaatacca cccacggtat gcaatgcatc ataaatatca aggactgcgc
    36181 ctccgggata accgaatact tgtttaacgc cctgatcgat aagcgatcgg acgaccatct
    36241 cggctccaga caacatctcc atggcctgcc tcactgtttg acggaaaaaa tgtgtaaaac
    36301 acattaaccg tccgatcttg cttaagcaat tggcagccag agacataaac acggggtaat
    36361 aagaataaac aggaggaaag gtgaaaagaa acgataaaat cctccattac agaggattaa
    36421 attgataata aatcggctga atcccacaac ttacagcaat ttctctattt ttttaattaa
    36481 tcctaaaata gagagcccac acattcagaa taaaattcta cattcaataa aaaatgcaga
    36541 ataaaccaga cattcatgtc tgacctattc tgcaatcagt tagcgtttgc aaattgagac
    36601 taattgctct tccatccact gatggccttt atcgcgcccg gcagcttcat gccaggagag
    36661 ataacaggtt ctgctgtttt gttttaacgg cagcggtaat acctgtaatt ctaaggattc
    36721 agcgaactct tcagccagcc aacgcggcgc aatagcgacc aaatgcgttt gcgacaccac
    36781 gctaagtacg ctcatcattg ccatgccctg atacgcgata ctggcttgct tatctaccgt
    36841 gtcataccaa ggttgactaa atgacgcgaa acgatcgagc gaaaccgccg catgttgttc
    36901 gttataaaca tcatgtttca gtaacgggcc cttaattgtt ggatgatttt tgctggctac
    36961 cagcaccatt tcatctttaa ataatggtac gctggtaaat tcaggacgat ggaagtcttc
    37021 ataactaatc acaaactccg tttcctgata acgcagctga tgttcagtgt tctgatttaa
    37081 tgaagacttg aacataacat gtatatttgg cgcaatctgc tcaatgtgat tataaatctg
    37141 cgaggtcaga atgctgtcta acgggctgca aacacaaaga tgaaatacac gttcactact
    37201 cgcgggttca aaacctgaac caggcaattc attttgtact agttgcaatg cctggcgaac
    37261 tgaaccaaaa agttgaaatg cgcgagcagt cggttgaata ccacggccat aacgaacaaa
    37321 aagctcgtca ttaaacatca ccttcaggcg tgcaacagcg ttactgaccg caggttgcga
    37381 catacccaga acatgagcgg cacgggtaat gttttgctcc tgcatcacgg catcgaaaac
    37441 ggttaataag ttgagatcga ccatgcgtag ctgtggcttg cttaactccg ccgtctctga
    37501 atgatctgtt tgtacctctg gcatacttaa ctccactgtc acacttaact ccctttccct
    37561 tattggaatg cagagaaata atcctgaaga atatgattta tcatgcatat aaaataagaa
    37621 aaaggaaaat gagtaaaatt aggaaaacat aaagatacgg gttctgataa aaacagaatc
    37681 cattgccgtg gaatgagtca tttaccacag cataataatc cataatgatt ttgttgcgaa
    37741 aacaatctaa tcataactac cgcgaatact caatcatcta caaaatggat taaatgtgct
    37801 tatttaataa ttaatttata tatttaatgc attaattctt aacattaatt gatcaataat
    37861 attcaccaaa tcaatatcaa aaaaaaaatc gcaaaacata taattcaata caaatcatca
    37921 ggataggttt tgcaacgcgt gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa
    37981 tttatgctga aatatgtcaa ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg
    38041 cttactttaa ccaccgcagc acaattagct aattttacgg atgcagaact cacgctggcg
    38101 ggacgttttt attgcgtcag ggttgacatc cgtttttgta tccagtaact ctaaaagcat
    38161 atcgcattca tctggagctg atttaatgac tcacatcgtt cgctttatcg gtctactact
    38221 actaaacgca tcttctttgc gcggtagacg agtgagcggc atccagcatt aagccagcac
    38281 gcagtcaaac aaaaaacccg cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct
    38341 ctaaaagaga caaggaccca aaccatgagc cagcaagtca ttattttcga taccacattg
    38401 cgcgacggtg aacaggcgtt acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg
    38461 ctggcccttg agcgtatggg tgttgacgtg atggaagtcg gtttccccgt ctcttcgccg
    38521 ggtgattttg aatcagtgca aaccatcgcc cgccaggtta aaaacagccg cgtatgcgcg
    38581 ttagctcgct gcgtggagaa agatatcgac gtggcggccg aatccctgaa agtcgccgaa
    38641 gccttccgta ttcatacctt tattgccact tcgccaatgc acatcgccac caagctgcgc
    38701 agcacgctgg atgaagtgat cgaacgcgct atctatatgg tgaaacgcgc ccgtaattac
    38761 accgatgatg ttgaattttc ttgcgaagat gccgggcgta cacccattgc cgatctggcg
    38821 cgagtggtcg aagcggcgat taatgccggt gccaccacca tcaacattcc ggacaccgtg
    38881 ggctacacca tgccgtttga gttcgccgga atcatcagcg gcctgtatga acgcgtacct
    38941 aacatcgaca aagccattat ttccgtacat acccacgacg atttaggcct ggcggtcggc
    39001 aactcactgg cggcggtaca tgccggtgca cgccaggtgg aaggtgcaat gaacgggatc
    39061 ggcgagcgtg ccggaaactg ttccctggaa gaagtcatca tggcgatcaa agttcgtaag
    39121 gatattctca acgtccacac cgccattaat caccaggaga tatggcgcac cagccagtta
    39181 gttagccaga tttgtaatat gccgatcccg gcaaacaaag ccattgttgg cagcggcgca
    39241 ttcgcacact cctccggtat acaccaggat ggcgtgctga aaaaccgcga aaactacgaa
    39301 atcatgacac cagaatctat tggtctgaac caaatccagc tgaatctgac ctctcgttcg
    39361 gggcgtgcgg cggtgaaaca tcgcatggat gagatggggt ataaagaaag tgaatataat
    39421 ttagacaatt tgtacgatgc tttcctgaag ctggcggaca aaaaaggtca ggtgtttgat
    39481 tacgatctgg aggcgctggc cttcatcggt aagcagcaag aagagccgga gcatttccgt
    39541 ctggattact tcagcgtgca gtctggctct aacgatatcg ccaccgccgc cgtcaaactg
    39601 gcctgtggcg aagaagtcaa agcagaagcc gccaacggta acggtccggt cgatgccgtc
    39661 tatcaggcaa ttaaccgcat cactgaatat aacgtcgaac tggtgaaata cagcctgacc
    39721 gccaaaggcc acggtaaaga tgcgctgggt caggtggata tcgtcgctaa ctacaacggt
    39781 cgccgcttcc acggcgtcgg cctggctacc gatattgtcg agtcatctgc caaagccatg
    39841 gtgcacgttc tgaacaatat ctggcgtgcc gcagaagtcg aaaaagagtt gcaacgcaaa
    39901 gctcaacaca acgaaaacaa caaggaaacc gtgtgatgtc gaagaattac catattgccg
    39961 tattgccggg ggacggtatt ggtccggaag tgatgaccca ggcgctgaaa gtgctggatg
    40021 ccgtgcgcaa ccgctttgcg atgcgcatca ccaccagcca ttacgatgta ggcggcgcag
    40081 ccattgataa ccacgggcaa ccactgccgc ctgcgacggt tgaaggttgt gagcaagccg
    40141 atgccgtgct gtttggctcg gtaggcggcc cgaagtggga acatttacca ccagaccagc
    40201 aaccagaacg cggcgcgctg ctgcctctgc gtaagcactt caaattattc agcaacctgc
    40261 gcccggcaaa actgtatcag gggctggaag cattctgtcc gctgcgtgca gacattgccg
    40321 caaacggctt cgacatcctg tgtgtgcgcg aactgaccgg cggcatctat ttcggtcagc
    40381 caaaaggccg cgaaggtagc ggacaatatg aaaaagcctt tgataccgag gtgtatcacc
    40441 gttttgagat cgaacgtatc gcccgcatcg cgtttgaatc tgctcgcaag cgtcgccaca
    40501 aagtgacgtc gatcgataaa gccaacgtgc tgcaatcctc tattttatgg cgggagatcg
    40561 ttaacgagat cgccacggaa tacccggatg tcgaactggc gcatatgtac atcgacaacg
    40621 ccaccatgca gctgattaaa gatccatcac agtttgacgt tctgctgtgc tccaacctgt
    40681 ttggcgacat tctgtctgac gagtgcgcaa tgatcactgg ctcgatgggg atgttgcctt
    40741 ccgccagcct gaacgagcaa ggttttggac tgtatgaacc ggcgggcggc tcggcaccag
    40801 atatcgcagg caaaaacatc gccaacccga ttgcacaaat cctttcgctg gcactgctgc
    40861 tgcgttacag cctggatgcc gatgatgcgg cttgcgccat tgaacgcgcc attaaccgcg
    40921 cattagaaga aggcattcgc accggggatt tagcccgtgg cgctgccgcc gttagtaccg
    40981 atgaaatggg cgatatcatt gcccgctatg tagcagaagg ggtgtaatca tggctaagac
    41041 gttatacgaa aaattgttcg acgctcacgt tgtgtacgaa gccgaaaacg aaaccccact
    41101 gttatatatc gaccgccacc tggtgcatga agtgacctca ccgcaggcgt tcgatggtct
    41161 gcgcgcccac ggtcgcccgg tacgtcagcc gggcaaaacc ttcgctacca tggatcacaa
    41221 cgtctctacc cagaccaaag acattaatgc ctgcggtgaa atggcgcgta tccagatgca
    41281 ggaactgatc aaaaactgca aagaatttgg cgtcgaactg tatgacctga atcacccgta
    41341 tcaggggatc gtccacgtaa tggggccgga acagggcgtc accttgccgg ggatgaccat
    41401 tgtctgcggc gactcgcata ccgccaccca cggcgcgttt ggcgcactgg cctttggtat
    41461 cggcacttcc gaagttgaac acgtactggc aacgcaaacc ctgaaacagg gccgcgcaaa
    41521 aaccatgaaa attgaagtcc agggcaaagc cgcgccgggc attaccgcaa aagatatcgt
    41581 gctggcaatt atcggtaaaa ccggtagcgc aggcggcacc gggcatgtgg tggagttttg
    41641 cggcgaagca atccgtgatt taagcatgga aggtcgtatg accctgtgca atatggcaat
    41701 cgaaatgggc gcaaaagccg gtctggttgc accggacgaa accaccttta actatgtcaa
    41761 aggccgtctg catgcgccga aaggcaaaga tttcgacgac gccgttgcct actggaaaac
    41821 cctgcaaacc gacgaaggcg caactttcga taccgttgtc actctgcaag cagaagaaat
    41881 ttcaccgcag gtcacctggg gcaccaatcc cggccaggtg atttccgtga acgacaatat
    41941 tcccgatccg gcttcgtttg ccgatccggt tgaacgcgcg tcggcagaaa aagcgctggc
    42001 ctatatgggg ctgaaaccgg gtattccgct gaccgaagtg gctatcgaca aagtgtttat
    42061 cggttcctgt accaactcgc gcattgaaga tttacgcgcg gcagcggaga tcgccaaagg
    42121 gcgaaaagtc gcgccaggcg tgcaggcact ggtggttccc ggctctggcc cggtaaaagc
    42181 ccaggcggaa gcggaaggtc tggataaaat ctttattgaa gccggttttg aatggcgctt
    42241 gcctggctgc tcaatgtgtc tggcgatgaa caacgaccgt ctgaatccgg gcgaacgttg
    42301 tgcctccacc agcaaccgta actttgaagg ccgccagggg cgcggcgggc gcacgcatct
    42361 ggtcagcccg gcaatggctg ccgctgctgc tgtgaccgga catttcgccg acattcgcaa
    42421 cattaaataa ggagcacacc atggcagaga aatttatcaa acacacaggc ctggtggttc
    42481 cgctggatgc cgccaatgtc gataccgatg caatcatccc gaaacagttt ttgcagaaag
    42541 tgacccgtac gggttttggc gcgcatctgt ttaacgactg gcgttttctg gatgaaaaag
    42601 gccaacagcc aaacccggac ttcgtgctga acttcccgca gtatcagggc gcttccattt
    42661 tgctggcacg agaaaacttc ggctgtggct cttcgcgtga gcacgcgccc tgggcattga
    42721 ccgactacgg ttttaaagtg gtgattgcgc cgagttttgc tgacatcttc tacggcaata
    42781 gctttaacaa ccagctgctg ccggtgaaat taagcgatgc agaagtggac gaactgtttg
    42841 cgctggtgaa agccaatccg gggatccatt tcgacgtgga tctggaagcg caagaggtga
    42901 aagcgggaga gaaaacctat cgctttacca tcgatgcctt ccgccgccac tgcatgatga
    42961 acggtctgga cagtattggg cttaccttgc agcacgacga cgccattgcc gcttatgaag
    43021 caaaacaacc tgcgtttatg aattaatccc cttgcccggt caaatgaccg ggctttccgc
    43081 tatcgtccac gtcatcaaac gtctttaacc tttgcggtta aaaataatgc gataacagaa
    43141 ataaccgcaa ttacccagta gacagcaaag tgcccccaac tttgtgcaat tgctccctga
    43201 ataacgccag ccagaattac cccggtagaa atactgttag taaataaggt ggtagctgcc
    43261 cccgctcttc caggcattaa atcctgaaac catagcatcc caatacccgc aacaatgccg
    43321 ataaatacag cgttaaaaag ttgcagcgtc atcaacgcca gacggctatg aaataaaatc
    43381 aatccggtgt aaaacagtac tcctgccgcc actgctatga ccatcattcg ccgcttacca
    43441 taacgtttga catagtagcc agccagaatc attgctggta tttccagacc agctgccgtc
    43501 cccatcagga aacccgccag tttgtctggc aatcctaact cgctactgat ccacaacggc
    43561 atatcaataa tgtacatggt gttgcaggtc cacattaacg tcgaggcgac aaataacatc
    43621 cgtacgttac tatcctgcca gccaccttgc attgataaag cattttccga cggcagttct
    43681 acccgcgcca cagacggaag cataaatgca atcaatacca gactgagtgt gaatatcccg
    43741 gcggcaatcg aaaacatcac cgtaaagccg taattcaacg ccagcataaa ggccaacggt
    43801 ggaccgataa cccatgccag agaaagctgc gcacgcatca ccgagctaaa catcaccact
    43861 tctcgcgccg agttatccgc atattcccgc gccagagcaa ataactgtgg cattgccgta
    43921 ttggccagag atgccagaag cacaccacag gtgataagcg tcagataatg acgattaaat
    43981 gcaaacaata gcgcattgcc gatagccatc aaacagcaaa atataatcag ttttcgccga
    44041 tcgccctgac tgtcagaacg ttttgccaac cagaggctta cgccgatccc agcaatagca
    44101 ttcaccgtat aaaagagtcc gatccagaaa ggttgcgcgc caacctcacg actcaggtat
    44161 aagctcaatg taggagcctg tagcgccccg gccaccccca tcataaaagc gaccagcata
    44221 aatgccgcgt aaacaccgtt catacggcga gccatcgtca ttatccagat catacgttcc
    44281 ctttttagcg cggcgagaat aaaaaaaacc agcaggtata atctgctggc gggtgatttt
    44341 acaccaatac tcagtcacac atgatgcagg caagtcaact ttcagaattg cggtcatccc
    44401 actgcatcag tccttcaagc atggttaatc gttgtgggac gcttaaccaa cgcaaccagc
    44461 acaacttcgc tgtcgcggta aaatagtgct gataaaactg acgcatgggg cacccccttg
    44521 cttcatcgtt aggaattatt gacttaatat agggaaaata aaattgctgt cttttgcaca
    44581 ggagttcccc ttttatgcca tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat
    44641 gctgcgaggg taaatcgcag gacacaacgc tcaacgaact ggcagcgtta ttgagctgct
    44701 cgcgtcgtca tatgcgcacc ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg
    44761 aagcggaagt cgggcgcggt aaacgctcgc gtctgacatt cctctatacc gggctggcgc
    44821 ttcagcaaca gcgggcggaa gacctgctgg agcaggatcg tatcgatcaa ctggtgcagt
    44881 tggttggcga caaagcgact gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc
    44941 gccaggggcg gcacatcctg cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg
    45001 gcagcgcatt gcgccgttcc gaaacccata tcgcccggca aatcttcagt tcgctaacgc
    45061 gcataaatga ggaaaatggg gaactggaag cagacatcgc ccaccactgg cagcaaattt
    45121 caccgcttca ctggcgtttc tttttgcgtc caggagtcca ttttcaccat ggtcgtgaac
    45181 tggaaatgga cgatgtgatc gcctctttaa aacgaatcaa tacgctgccg ctctattcgc
    45241 atattgctga cattgtgtcg ccgacgccct ggacgctgga tatccatctc acgcaaccgg
    45301 accgctggtt accgttactg ctggggcaag ttccggcgat gatcctgccg cgcgaatggg
    45361 aaaccctcag taactttgcc agccatccca tcggcaccgg tccgtatgcg gtgattcgca
    45421 acagcaccaa tcaactgaaa attcaggcat tcgatgactt cttcggttac cgggcattaa
    45481 tcgacgaagt taacgtctgg gttctgccgg aaattgccga cgagccagcc ggagggctga
    45541 tgctaaaagg tccacagggc gaggaaaaag agattgaaag ccgcctggag gaaggttgct
    45601 actatttact gttcgacagc cgcacccatc gcggggcgaa tcagcaagtc agggactggg
    45661 taagctatgt gctttctcca actaatctgg tctatttcgc tgaggaacag taccagcaac
    45721 tgtggttccc ggcttatgga ctgctccccc gttggcacca tgcccgcacc ataaagagcg
    45781 aaaaaccggc tggcctggaa agcctcaccc taacctttta tcaggatcac agtgagcatc
    45841 gggtgattgc cgggatcatg cagcagattc tggcaagtca ccaggtcacg ctgaaaatca
    45901 aagagatcga ctacgatcag tggcatacag gagagatcga aagtgatatc tggctaaaca
    45961 gcgccaactt taccctgccg ctggacttct ctgttttcgc acatttatgc gaagtgccac
    46021 tgctacaaca ttgcattccc attgactggc aagccgacgc tgctcgctgg cgcaatggcg
    46081 agatgaatct ggcgaactgg tgccagcaac tggtcgccag caaagcgatg gtgccattat
    46141 tgcaccactg gctgatcatt caggggcaac gcagtatgcg cggcctgcgc atgaataccc
    46201 tcggctggtt cgattttaaa tcagcgtggt ttgcgccacc ggatccatga ttgctggttg
    46261 ataacaaaat cactacacta acgccgttct caacggggtg ccacgcgtac gcgtgcgctg
    46321 agaaaatacc cgtcgaacct gatccggata acgccggcga agggatttga ggctccttct
    46381 caagtccttt gccactcttt tttgaggtgc aaagtgttaa aaaaatgtct gcccctgctg
    46441 ttgctgtgca cagcgcccgt tttcgctaaa cccgttctga ctgtttatac ctacgattcc
    46501 ttcgccgccg actgggggcc tggtccggtg gttaaaaaag cctttgaagc cgactgtaat
    46561 tgcgaactga aactggtggc gctggaagat ggcgtttcgc ttctcaaccg tctacggatg
    46621 gaaggcaaaa acagtaaagc cgatgtggtg ctggggctgg ataacaacct gttagacgcc
    46681 gccagtaaaa ccggactgtt tgccaaaagc ggtgtggcag cggatgccgt taacgttccc
    46741 ggcggctgga ataatgacac tttcgtaccg tttgattatg gctacttcgc cttcgtttat
    46801 gacaagaaca aactgaaaaa cccgccacaa agcctgaaag aactggttga gagcgatcaa
    46861 aactggcggg tgatttatca ggatccgcgc accagtacac cggggctggg tctgttgcta
    46921 tggatgcaaa aagtctatgg cgatgacgcc ccacaagcct ggcagaaact ggcgaagaaa
    46981 acggtcacgg tcaccaaagg ctggagcgaa gcctacggcc tgtttttaaa aggtgaaagc
    47041 gatctggtac tgagttacac cacctctccg gcttatcaca ttctcgaaga gaagaaagat
    47101 aactacgccg ccgcgaactt cagcgaaggt cactatctgc aagtggaagt cgccgcccgc
    47161 accgctgcca gcaagcagcc ggagctggcg caaaaattcc tccagtttat ggtttctccg
    47221 gctttccaga atgcgatccc aaccggcaac tggatgtatc cggtggcaaa cgtcacgctg
    47281 cctgccggtt ttgaaaaatt gaccaaaccc gcaaccacgt tggagttcac gccagccgaa
    47341 gtggcggcac aacgtcaggc atggattagc gaatggcaac gcgccgtcag ccgttaattc
    47401 ccggctggtt aattccaggt gtaagcgcca ccacgctggt ggtagcggtt gcgctggcgg
    47461 cgtttctcgc cctgtggtgg aacgcgccgc aggatgactg ggtggcagtc tggcaggaca
    47521 gctatctgtg gcatgtggtg cgcttctcct tctggcaggc gtttctctcg gcactgctct
    47581 ctgtcatacc cgcgatattc ctcgcccgcg cgctctatcg caggcgcttt ccgggtcggc
    47641 tggcgctgtt gcgtctgtgt gcaatgacct tgatcctccc ggtgttggtc gctgttttcg
    47701 gcattcttag cgtctatggt cgccagggct ggctggcaac actctgccaa tcgctcggtc
    47761 tggagtggac cttttcgccc tacggcctgc aaggtatttt gctggcccat gtgtttttta
    47821 atctgccgat ggcgagccgc ttattactcc aggcactgga aaacatcccc ggcgaacagc
    47881 gtcaacttgc cgcccagctt gggatgcgta gctggcattt tttccgcttc gtcgaatggc
    47941 cgtggttacg gcgacaaatc ccgccggttg ctgcgcttat ctttatgctc tgtttcgcca
    48001 gcttcgccac cgtgctatcg ctggggggcg gtccgcaggc gaccactatc gagctggcaa
    48061 tctatcaggc gctgagttac gactacgatc ctgcccgcgc ggcaatgctg gcgctgctcc
    48121 agatggtgtg ctgcctcggg ctggtgctgt tgagtcagcg attgagtaag gccattgcgc
    48181 ccggcaccac gctgctgcaa ggctggcgcg acccggacga tcgtctgcat agccgcattt
    48241 gcgacacggt gttaattgtg ctggcgctgc tgctgttgct gccaccgtta ctggcggtga
    48301 tcgtcgatgg ggtaaatcgc cagttgccgg aagtgctggc acaaccggtg ctgtggcagg
    48361 cgctgtggac ctcgttgcgt attgcgctgg cggcaggtgt attgtgcgta gtgctgacca
    48421 tgatgctgct atggagcagt cgcgaactgc gggcgcggca gaaaatgctg gcgggtcagg
    48481 tgctggagat gagcggcatg ttgatcctcg ccatgccggg gattgtgctg gctaccggct
    48541 tctttttact gctcaacaac actatcggcc tgccacaatc tgctgacggc attgtgattt
    48601 tcaccaatgc gttaatggcg atcccttatg cgctgaaagt gctggaaaac ccgatgcgcg
    48661 atatcaccgc ccgctacagc atgttatgtc agtcgctggg gattgaaggc tggtcacgct
    48721 taaaagtggt ggagctgcgc gccctgaaac gtccactggc gcaggcgctg gcctttgcat
    48781 gcgtgctgtc gattggtgat tttggcgtgg tggcgttgtt cggtaacgat gatttccgca
    48841 ccctgccgtt ttatctctac cagcaaattg gctcctatcg cagccaggac ggtgcggtca
    48901 ccgcgttaat tctgctgctg ctctgttttc tgctgtttac cgtgattgaa aaactaccgg
    48961 ggcgaaatgt taaaactgac tgatatcacc tggctttacc accatttgcc gatgcgtttt
    49021 agcttaacgg tggaacgcgg cgagcaggtg gcgatcctcg ggccaagcgg cgcgggtaaa
    49081 agtaccctgc tgaatttgat cgccggtttt ctgacgccag ccagcggttc gctgactatc
    49141 gatggcgtag atcacacaac tatgccgccg tcacgccgtc cggtgtcgat gctgtttcag
    49201 gagaacaacc tgttcagcca cctgacggtc gcacagaaca tcgggctggg gctaaatccg
    49261 ggattgaaac tgaacgcggt acagcagggg aaaatgcacg ctatcgcccg ccagatgggg
    49321 attgataatt taatggcgcg gttaccgggc gagctttccg gcggtcagcg acagcgagtg
    49381 gcgttagcgc gttgtctggt acgcgaacag ccgattttat tgctcgatga accgttctct
    49441 gcgctcgatc cggcgttacg tcaggagatg ttgacgctgg tgagcacgag ctgccagcag
    49501 caaaaaatga cgctattgat ggtgtcgcac agcgtggaag atgcggcgcg gatcgccacg
    49561 cgctcggtag tagtcgccga cgggcgcatc gcctggcagg gtatgaccaa tgagttgttg
    49621 agcggtaagg cgagtgcttc ggcactattg gggattacgg gttagtatgc actgtcggat
    49681 gcggcgtgaa cgccttatcc gacctacggg tagtgcgttt tgtaggcctg ataagacgcg
    49741 gcaagcgtcg catcaggcaa cccgcacaag actatctcct aaaccccaac cactttacgc
    49801 aaaatatcga tatacaccgg catcaacggg tggcgaatta acaccaccag cgccaccacg
    49861 ccgatggcag aaatcaacgg cgtcagccac aacaaacgac cgcgggacaa ataatgactc
    49921 aaacggtcag tcgctttacc gctgcgccat aaccgccagc acagccagcc accaacccac
    49981 aaaaacaccg ctgttgccag cagcaaccat ttaaactcac cgctctgcat tccggcagga
    50041 atatcgatcg ccgcgcccgc cagaatccct ggcaggaagt aaaacggcgg ccacagcagg
    50101 cagccgataa tattcggcgt aataaattta gccaccggca gatccagcat tcccgccacc
    50161 attggcacca gcggacgcgt cgggccaaca aaacgaccga ccagaatggt gaacatgctg
    50221 tgttgatgca acgcatgttc agttttatca agtagtgctt tgtttttctt cagaaatgac
    50281 cagcgatgca acggcttttt aaaacgccaa cccagccaga aagaaatcca gtcgcccatc
    50341 aagcagccaa taatccctgc cagccaggcg tgccagaaac ttaactcgcc gctgccaatc
    50401 agcgctccca gccccgccat cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc
    50461 gactccagaa aggccaccaa caccaccgcc atcaatgaat acacggtgga ttgggtaata
    50521 aagtgttcca gcaatgcttg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
    50581 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
    50641 gacggctaca tcattcactt tttcttcaca accggcacga aactcgctcg ggctggcccc
    50701 ggtgcatttt ttaaatactc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
    50761 gacggtggcg ataggcatcc gggtagtgct caaaagcagc ttcgcctgac taatgcgttg
    50821 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
    50881 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
    50941 gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga
    51001 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
    51061 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
    51121 atgcggctgg tgcgcttcat ccgggcgaaa gaaacccgta ttggcaaata ttgacggcca
    51181 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
    51241 gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
    51301 acccggtcgg cagacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
    51361 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
    51421 gttggcctca atcggcgtta aacccgccac cagatgggcg ttaaacgagt atcccggcag
    51481 caggggatca ttttgcgctt cagccatact tttcatactc ccaccattca gagaagaaac
    51541 caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc tcttctcgct
    51601 aacccaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga ccaaagccat
    51661 gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca ttgattattt
    51721 gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag cggatcctac
    51781 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt ggatggagtg
    51841 aaacgatggc gattgcaatt ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg
    51901 tggactgcgc taccggtgaa gagatcgcca ccagcgtaga gtggtatccc cgttggcaga
    51961 aagggcaatt ttgtgatgcc ccgaataacc agttccgtca tcatccgcgt gactacattg
    52021 agtcaatgga agcggcactg aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag
    52081 ctgtggtcgg gattggcgtt gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg
    52141 gaaacgtgct ggcgctgcgc ccggagtttg ccgaaaaccc gaacgcgatg ttcgtattgt
    52201 ggaaagacca cactgcggtt gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg
    52261 gcaacgttga ctactcccgc tacattggtg gtatttattc cagcgaatgg ttctgggcaa
    52321 aaatcctgca tgtgactcgc caggacagcg ccgtggcgca atctgccgca tcgtggattg
    52381 agctgtgcga ctgggtgcca gctctgcttt ccggtaccac ccgcccgcag gatattcgtc
    52441 gcggacgttg cagcgccggg cataaatctc tgtggcacga aagctggggc ggcctgccgc
    52501 cagccagttt ctttgatgag ctggacccga tcctcaatcg ccatttgcct tccccgctgt
    52561 tcactgacac ttggactgcc gatattccgg tgggcacctt atgcccggaa tgggcgcagc
    52621 gtctcggcct gcctgaaagc gtggtgattt ccggcggcgc gtttgactgc catatgggcg
    52681 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
    52741 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
    52801 aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
    52861 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
    52921 agcatccgga actgaaaacg caaatcaacg ccagccagaa acaactgctt ccggcgctga
    52981 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
    53041 acggccgccg cacaccgaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
    53101 tcgctaccga cgctccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
    53161 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt taataacgtg atggcactgg
    53221 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
    53281 cgctgcaaat tgttgcctct gatcagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    53341 tcgccgcgaa agtgcacgca gacatcccat cagctcagca aaaaatggcc agtgcggtag
    53401 agaaaaccct gcaaccgtgc agcgagcagg cacaacgctt tgaacagctt tatcgccgct
    53461 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    53521 ctgcccaggc cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa
    53581 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    53641 caacatgccg agcacgtcgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg
    53701 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    53761 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    53821 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    53881 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    53941 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc
    54001 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    54061 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    54121 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    54181 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    54241 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcaaa
    54301 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    54361 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    54421 cctggtctgg ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    54481 tggaaaactg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    54541 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    54601 tcccatatgc tggaagtctg cccgtcgatc gccgcagaag agaaaccgat cctcgacgtt
    54661 cagcatctcg gtattggtgg taaggacgat cctgcccgcc tgatcttcaa tacccaaacc
    54721 ggcccagcga ttgtcgccag cttgattgat ctcggcgatc gttaccgtct actggttaac
    54781 tgcatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc gaatgcgctg
    54841 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    54901 caccataccg tcttcagcca tgcactgaac ctcaacgata tgcgccaatt cgccgagatg
    54961 cacgacattg aaatcacggt gattgataac gacacacgcc tgccagcgtt taaagacgcg
    55021 ctgcgctgga acgaagtgta ttacgggttt cgtcgctaag tagccgcatc cggtatgtaa
    55081 cgcctgatgc gacgctgacg cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    55141 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    55201 acgcgttccg attttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc
    55261 gacgctggcg cgtcttatca ggcctacacg ctgcgatttt gtaggccgga taagcaaagc
    55321 gcatccggca cgaaggagtc aacatgttag aagatctcaa acgccaggta ttagaggcca
    55381 acctggcgct gccaaaacat aacctggtca cgctcacatg gggcaacgtc agcgccgttg
    55441 atcgcgagcg cggcgtcttt gtgatcaaac cttccggcgt cgattacagc atcatgaccg
    55501 ctgacgatat ggtcgtggtt agcatcgaaa ccggtgaagt ggttgaaggt gcgaaaaagc
    55561 cctcctccga tacgccaact caccgactgc tctatcaggc attcccgtcc attggcggca
    55621 ttgtgcacac acactcgcgc cacgccacta tctgggcgca ggcgggccag tcgattccag
    55681 caaccggcac cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga
    55741 ccgacgcaga aatcaacggt gaatatgagt gggaaaccgg taacgtcatc gtagaaacct
    55801 tcgaaaaaca gggtatcgat gcagcgcaaa tgcccggcgt cctggtccat tctcacggcc
    55861 catttgcatg gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg
    55921 tcgcttatat ggggatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa
    55981 cgctgctgaa taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat
    56041 gactgtataa aaccacagcc aatcaaacga aaccaggcta taatcaagcc tggttttttg
    56101 atggaattac agcgtggcgc aggcaggttt tatcttaacc cgacactggc gggacacccc
    56161 gcaagggaca gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct
    56221 tgcaccgcaa gagtccgtgg cgtttattcc cgccgatcag gttccccgcg ctcagcatat
    56281 tttgcagggt gaacaaggct ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca
    56341 gccggtgtat ggcctttact gtcgcgccca tcgccaattg atgaattacg aaaagcgcct
    56401 gcgtgaaggt ggcgttaccg tctacgaggc cgatgtgcgt ccgccagaac gctatctgat
    56461 ggagcggttt atcacctcac cggtgtgggt cgagggtgat atgcacaatg gcactatcgt
    56521 taatgcccgt ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctataga
    56581 tattgaaacc acccgccacg gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg
    56641 catcgtttat atgctggggc cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga
    56701 atacgtcacc agccgcccgc agttgctgga aaaactcaac gcctggtttg ccaactacga
    56761 tcctgatgtg atcatcggtt ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca
    56821 tgccgagcgt taccgtcttc cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg
    56881 cgagcacggc tttaaaaacg gcgtcttttt tgcccaggct aaaggtcggc taattatcga
    56941 cggtatcgag gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt
    57001 cgctcaggag ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga
    57061 aattgaccgc cgtttcgccg aagataaacc tgcgctggca acttataacc tgaaagattg
    57121 cgagctggtg acgcagatct tccacaaaac tgaaatcatg ccatttttac tcgaacgggc
    57181 aacggtgaac ggcctgccgg tggaccgcca cgggggatcg gtggcggcgt ttggtcatct
    57241 ctattttcct cgtatgcacc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc
    57301 acacgccagc cctggcggct acgtgatgga ttcacggcca gggctttatg attcggtact
    57361 ggtgctggac tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt
    57421 cgggctggtg gaaggcatgg cgcagcctga tccagagcac agcaccgaag gctttctcga
    57481 tgcctggttc tcgcgggaga aacattgcct gccggagatt gtgactaaca tctggcacgg
    57541 gcgcgatgaa gccaaacgcc agggtaacaa accgctgtcg caggcgctga aaatcatcat
    57601 gaatgccttt tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcggctggc
    57661 atcgtcgatc accatgcgtg gtcatcagat catgcggcaa accaaagcgt tgattgaagc
    57721 acagggctac gacgttatct acggcgatac cgactcaacg tttgtctggc tgaaaggcgc
    57781 acattcggaa gaagaagcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg
    57841 gtgggcggaa acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac
    57901 ccatttctgc cgttttctga tgccaaccat tcgcggagcc gataccggca gtaaaaagcg
    57961 ttatgccgga ctgattcagg agggcgacaa gcagcggatg gtgtttaaag ggctggaaac
    58021 cgtgcgcacc gactggacgc cgctggccca gcagtttcag caggagctat acctgcgcat
    58081 cttccgcaac gagccatatc aggaatatgt acgcgaaacc atcgacaaac tgatggcggg
    58141 tgaactggat gcgcgactgg tttaccgtaa acgccttcgc cgtccgctga gcgagtatca
    58201 gcgtaatgtg ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg
    58261 tggtcgcccc ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccaacgg
    58321 cccggagccg ctggactacc aacgttcacc actggattac gaacactatc tgacccgcca
    58381 gctacaaccc gtggcggagg gaatactccc ttttattgag gataattttg ctacacttat
    58441 gaccgggcaa cttgggctat tttgagcaaa aaaaagagtt cgccagatac cattttgatg
    58501 cgtgacgaat gctttgccat ccagtaccat agcgcccttt ccattcctgg acctgaataa
    58561 caccactacc tcataagcac ggtagcgggt ggttattgcc tgcaattaaa gatatagagc
    58621 cgaacacata tgccttttac acttggtcaa cgctggatca gcgatacaga aagcgaattg
    58681 ggacttggaa ccgttgtcgc ggtggatgcg cgaactgtca ctttactttt cccatctact
    58741 ggtgaaaacc gtctgtacgc acgcagtgat tcccccgtga cccgcgtgat gttcaaccct
    58801 ggtgatacca ttaccagcca tgacggctgg cagatgcaag tcgaagaagt aaaagaagaa
    58861 aatggcttgc tgacctatat cggtactcgc ctggatactg aagagtccgg cgtagccctg
    58921 cgtgaagttt tccttgatag caaactggtg ttcagcaaac cgcaggaccg tctgtttgcc
    58981 gggcagattg accgtatgga ccgctttgcg ctgcgttatc gcgcgcgtaa atattccagc
    59041 gaacagttcc gtatgccgta cagcggcctg cgcggtcagc gtaccagcct gatcccgcat
    59101 cagctcaaca tcgctcatga tgttggtcgc cgccacgcgc cgcgcgtcct gctggctgac
    59161 gaagtgggtt tagggaaaac cattgaagcc gggatgatcc tgcatcagca actgctctct
    59221 ggcgctgctg aacgtgtgct aattatcgtc ccggaaacct tacagcatca gtggctggta
    59281 gaaatgctgc gccgtttcaa cctgcgcttt gcgctatttg atgatgagcg ttatgccgaa
    59341 gctcagcacg atgcttacaa cccgtttgac accgaacagc tggtgatttg ctcgctggat
    59401 tttgcccgtc gtagcaaaca gcgcctggaa catctctgtg aagccgaatg ggacctgctg
    59461 gtggtcgatg aagcgcatca cctggtgtgg agcgaagatg cgccaagccg tgaatatcag
    59521 gccattgaac aactggcaga gcacgtgccg ggcgttctgc tgctgaccgc gaccccggaa
    59581 cagctgggga tggaaagcca cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac
    59641 gattttgcgc agttcgttga agagcagaaa aattatcgtc cggttgcgga cgccgttgcc
    59701 atgctgctgg caggtaacaa actgagcaat gacgaactga acatgctcgg cgagatgatc
    59761 ggcgagcagg atatcgagcc gctgttgcaa gcagcaaaca gcgacagcga agatgcccag
    59821 agcgcccgtc aggaactggt ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg
    59881 ttccgtaaca cccgtaacgg tgtgaaagga ttcccgaaac gcgagctgca caccattaag
    59941 ctgccgctac cgacgcagta tcagacggct attaaagtct ccggcattat gggcgcacgt
    60001 aaaagtgcgg aagatcgtgc tcgcgatatg ctctacccgg agcgtattta tcaggaattt
    60061 gaaggtgata acgccacctg gtggaacttc gatccgcgcg ttgagtggct gatgggctac
    60121 ctgaccagcc atcgctctca gaaagtgctg gtgatctgcg ctaaagctgc cactgcgctg
    60181 caactggagc aagtactgcg cgaacgtgaa ggtatccgcg ctgctgtgtt ccacgaaggc
    60241 atgtcgatta ttgagcgtga ccgcgctgcc gcctggtttg ctgaagaaga caccggcgca
    60301 caggtactgc tgtgctcgga aatcggttct gaaggacgta acttccagtt cgccagccac
    60361 atggtgatgt ttgacctgcc attcaacccg gatctactgg agcagcgtat tggtcgtctg
    60421 gatcgtatcg gccaggcgca cgatattcag atccatgtgc cttatctgga gaaaaccgct
    60481 cagtcggtgc tggtgcgctg gtatcacgaa ggtctggatg catttgagca cacctgcccg
    60541 accggacgca ctatttacga tagcgtatac aacgatctga ttaactatct ggcttcaccg
    60601 gatcaaaccg aaggctttga cgatctgatc aaaaactgcc gcgagcaaca tgaagcgctg
    60661 aaagcacagc tggaacaggg tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa
    60721 aaagcccagg cactggcaga aagcattgaa gagcaggatg acgataccaa cctgatcgcc
    60781 ttcgccatga acctgttcga tattatcggt atcaatcagg acgatcgcgg cgacaacatg
    60841 atcgtgctga cgccgtccga tcatatgctg gtgccggact tccctggcct gtcggaagat
    60901 ggcatcacca tcacctttga tcgtgaagtg gcgctggcgc gtgaagatgc acagtttatt
    60961 acctgggagc atccgctgat ccgcaacggt ctggatctga tcctttctgg cgataccggt
    61021 agcagcacga tttcactgtt aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa
    61081 ctgatttatg tggttgaagc ccaggctccg aagcagttgc agctcaaccg cttcctgcca
    61141 ccgacgccgg tacgtatgct gctggataaa aacggcaaca acctggcggc gcaggtagag
    61201 tttgaaacct ttaaccgcca gcttaacgcg gttaaccgtc acaccggcag caaactggtt
    61261 aacgccgtgc agcaggatgt tcacgctatc cttcaactgg gtgaagcgca gatcgagaaa
    61321 tctgcccgtg cattgattga tgcagcgcgt aacgaagccg acgaaaaact gtctgccgag
    61381 ctgtctcgtc tggaagctct gcgtgcagtg aacccgaaca ttcgtgacga cgaactgacc
    61441 gccattgaga gcaaccgtca gcaggtaatg gaaagcctgg atcaggcagg ttggcgtctg
    61501 gatgccctgc gtttgatcgt tgtaacgcat cagtaacgga gccgaaaatg gggatggaaa
    61561 actacaatcc accgcaggaa ccctggttgg ttatcctgta tcaggatgac catattatgg
    61621 tggtcaacaa gccgagcggt ttgttgtcag tgccgggtcg tctggaagag cacaaagaca
    61681 gcgtgatgac gcgcattcag cgtgattatc cgcaggcaga atcggtgcat cgtctggata
    61741 tggctaccag cggcgtgatt gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac
    61801 gccagttccg cgagcgcgag ccgaaaaagc agtatgtggc ccgcgtctgg gggcatccat
    61861 cccccgcaga aggtctggtg gatctgccgc tgatttgcga ctggccaaac cgcccgaaac
    61921 agaaagtctg ttacgaaacg ggtaaacctg cgcagacgga atatgaagtg gtggagtatg
    61981 cggcggataa cacggcaaga gtggtgttaa aaccgattac cgggcgttcg catcaattgc
    62041 gtgtgcatat gctggcgctg gggcatccga ttctcggcga tcgtttttat gcatcaccag
    62101 aagcgagagc gatggcaccg cgtttgttgc tacatgcaga gatgttgacg attacccatc
    62161 cggcgtatgg caatagtatg acgtttaaag cgccagcgga tttttaatcg tcacgctggc
    62221 gcagttcggt aggcggataa ggcgttcacg ccgcatccgg caattgtgcg ccgattgcct
    62281 gatgcgacgc gggccgcgtc ttatcaggcc tacagtttaa aacttattta aaccctttct
    62341 gctgctttat cagctcatat gcctgctgaa tttcctgcgc tttctgcttc gccatctcca
    62401 tcatctcagg cggcaaacct ttcgccacca gcttatcggg atggtgttca ctcatcagct
    62461 tacggtaggc acgtttgatg gtggtcgcat catccgtcgg cttcacgccc agcacattac
    62521 aggcatcttc cagcgttggg ccacgctgcg cttgctgcca gttaccaccg ccagtttgct
    62581 gctgataacc gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact
    62641 gagcgcggga gatccctaat tcttctgcaa tgacatacag caccgcccgt tcattcgggt
    62701 gcagtgaacc atcagcaaac gccgcctgaa tctggatctc cagaaacata cgaattaagt
    62761 caaaacgacc aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattgt
    62821 ctgattttcc cacccggaac gcattttgcg ccgcagtacg ggaagcgcca tgaagattca
    62881 ttcggtccat caactggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt
    62941 tggttaaatg ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct
    63001 ggttggcgaa ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca
    63061 ggcctaacac tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga
    63121 tttttcccca atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc
    63181 acaatataaa ggtgctttta ccgttttccg gctgcggtca attgtgggac atcgcctata
    63241 atttgcatta tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac
    63301 aggattaaca ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca
    63361 acgttaccga tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg
    63421 gccaccatga ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca
    63481 cagtgcatgt tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac
    63541 ttacccgtga ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt
    63601 actggcagcg tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc
    63661 catcaaaaag aggcaccagg acaaccggag ccggtacgaa ccgttgatgc gctcggtaat
    63721 gtccattacg acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac
    63781 accaaagata ccaacgtctg ggaaggtgat taccagatgg tgggtcgcca gggtcgcggt
    63841 aaagcggacc tgatgaaaca acgtggcgaa aaccgctata ccattctgga taacggtagc
    63901 tttacctcct gtctgccggg ttctgacacc tggagcgtgg taggtagcga aattattcat
    63961 gaccgcgaag aacaagttgc ggagatctgg aacgcccgct ttaaggtggg tccggtaccg
    64021 atcttttata gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg
    64081 atcccgaacg ccaagtacac caccaccaac tactttgagt tctacctgcc atattactgg
    64141 aacatcgcgc caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaac
    64201 atcatgtggg agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg
    64261 gactatctgc cttcagataa agtctatgaa gatgaacacc cgaacgatga cagttcacgt
    64321 cgttggttat tctactggaa ccactccggg gtcatggatc aggtgtggcg tttcaacgtc
    64381 gactacacca aggtcagcga tcctagctac ttcaatgatt tcgataacaa gtacggttcc
    64441 agtactgacg gctacgcaac gcaaaaattc agcgttggct atgcggtgca aaacttcaat
    64501 gccaccgttt caaccaagca gttccaggtt ttcagcgaac agaacaccag tagctactcg
    64561 gcagagccgc agttagacgt taattactac cagaatgatg ttggtccgtt tgatacgcgt
    64621 atttacggcc aggcagtgca ctttgttaac accagagacg acatgcctga agcaacccgt
    64681 gttcacctgg aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc
    64741 gaagcgaagt tgctggcaac ccattatcag caaaccaatc ttgactggta taactccaga
    64801 aacacgacca agctggacga atccgttaac cgcgtaatgc cgcaattcaa agttgacggc
    64861 aaaatggtct ttgaacgcga tatggaaatg ctggctccgg gttataccca aacgctggaa
    64921 ccgcgcgcgc agtatttgta cgtgccgtat cgcgatcaga gcgacatcta taactacgac
    64981 tcgtctctgc tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt
    65041 gaccgtattg cctccgctaa ccaggtgacg accggtgtca catctcgcat atatgatgat
    65101 gctgccgttg aacgttttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc
    65161 actggcgatg acaacataac atgggagaat gacgacaaaa cgggttcact ggtgtgggca
    65221 ggcgatactt actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat
    65281 acacgtctgg ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac
    65341 cgtctggtac agctgaatta ccgttacgcc agcccggaat atattcaggc tacgctgcct
    65401 aagtactatt ccactgctga gcaatataag aatggtattt cgcaggtagg tgctgtcgcc
    65461 agctggccaa ttgccgatcg ttggtccatt gttggggcct actactacga caccaatgcg
    65521 aacaagcaag ccgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc
    65581 gtcggttacg agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac
    65641 aacgcaatcg gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg
    65701 caagagatgc tgcgttcgaa cattctgccg tatcaaaaca ctttgtgatt tgttgattta
    65761 ccacgtaatc cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct
    65821 gcttctcggt atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa
    65881 agtcgcagcc gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca
    65941 gtcggtaaaa ctgaacgctg ctcaggcaag gcagcaactt cctgatgacg cgacgctgcg
    66001 ccaccaaatc atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat
    66061 gggagtgaaa atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa
    66121 caacatgacg ctggatcaga tgcgcagccg tctggcttac gatggactga actacaacac
    66181 ctatcgtaac cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg
    66241 tcgtcgcatc accatcctgc cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca
    66301 aaacgacgcc agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc
    66361 gacctctgat caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg
    66421 taacggcgct gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa
    66481 cggcggccag atgggctggg gccgtattca ggagttgccc gggatcttcg cccaggcatt
    66541 aagcaccgcg aagaaaggcg acattgttgg cccgattcgt tccggcgttg gcttccatat
    66601 tctgaaagtt aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc
    66661 tcgccatatt ctgctgaaac cgtcgccgat catgactgac gaacaggccc gtgtgaaact
    66721 ggaacagatt gctgctgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga
    66781 gttctctcag gatccaggct ctgctaacca gggcggtgat ctcggctggg ctacaccaga
    66841 tattttcgat ccggccttcc gtgacgccct gactcgcctg aacaaaggtc aaatgagtgc
    66901 accggttcac tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga
    66961 taaaaccgac gctgcgcaga aagatcgtgc ataccgcatg ctgatgaacc gtaagttctc
    67021 ggaagaagca gcaagctgga tgcaggaaca acgtgccagc gcctacgtta aaatcctgag
    67081 caactaatgg ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc
    67141 ccggacttag ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc
    67201 gatgccactc tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgccct
    67261 tattccccca actcccctgc acaaccgcaa actacgggca cattaacgct acttcctgtc
    67321 gcgctacgtg aatctgtcac tgcggggcag ttagcgattg aaaatgggca ttacgtggtg
    67381 gagacgctgg cgcgagcgtg tgatggctgt ctgaacggtg aatttgctgc gctgatcaca
    67441 ggccccgtgc ataaaggcgt cattaacgac gcaggcattc cgtttaccgg tcataccgag
    67501 tttttcgaag agcgttcgca ggcgaaaaag gtggtgatga tgctggcgac cgaagaactt
    67561 cgcgtggcgc tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct
    67621 gcacttttgc acgaagtgat tgctattttg catcacgatt tgcgtaccaa atttggtatt
    67681 gccgaaccgc gcattctggt ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg
    67741 ggtacggaag agatagacac cattattccg gtgctcgacg agctgcgggc gcaggggatg
    67801 aaactcaacg ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc
    67861 gacgccgtgc tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc
    67921 gggcgcggtg tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc
    67981 accgcgcttg aactggcggg acgtggcaaa gccgatgtcg gcagttttat tacggcgctt
    68041 aatctcgcca tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact
    68101 tagcccgtaa acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagtattg
    68161 tgtctgccat taacccgcaa aagggccagg cgatggtcga aatcggcccc ggtctggcgg
    68221 cattgaccga accggtcggc gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg
    68281 atctggcggc acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc
    68341 aggatgcgat gacctttaac tttggtgaac tggccgagaa aatgggtcag ccgctgcgtg
    68401 ttttcggcaa cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata
    68461 ctgatgccat tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg
    68521 caggaccgaa cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaatg
    68581 tgatcccggt actggaagta ccgccgtcag cctttacacc accacccaaa gtggattccg
    68641 ccgtcgtgcg cctggttcct catgcaacga tgcctcaccc ggttaaagat gttcgtgtgt
    68701 tgagccgcat caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg
    68761 gcaacctgtt tagcgtcgag gtgttaacgg gaatggggat cgacccggcg atgcgagcgg
    68821 aaaatatctc tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt
    68881 tgcaggagag ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta
    68941 cattgaggct caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat
    69001 acgcaatctg gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg
    69061 caatggccgt gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc
    69121 gcctggcgaa gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat
    69181 gcagggtcac tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt
    69241 attccgactc gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg
    69301 cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc
    69361 tgggaaagat accctctggc tgacgggcga tctggtcgcg cgcgggccgg gttcgctgga
    69421 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga
    69481 tctgcatctg ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac
    69541 accgctgctg gaagcgccgg atgccgacga gctgcttaac tggctgcggc gccagcctct
    69601 gctgcaaatc gacgaagaga aaaagctggt gatggcccac gcagggatca cgccgcagtg
    69661 ggatctgcag accgccaaag agtgcgcacg cgatgtagaa gcggtgctat cgagtgactc
    69721 ctatcccttc tttcttgatg ccatgtacgg cgatatgcca aataactggt caccggaatt
    69781 gcgggggctg ggaagactgc gttttatcac caacgctttt acccgtatgc gtttttgctt
    69841 cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccact
    69901 gaaaccgtgg tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca
    69961 ctgggcatcg ctggagggca aaggtacgcc ggaaggtata tacgcgctgg ataccggctg
    70021 ctgctggggt ggtacattaa cctgcctgcg ctgggaagat aaacagtatt ttgtccagcc
    70081 gtcgaaccgg cataaggatt tgggcgaagc ggcggcgtct taaacacagc ctgatatagg
    70141 aaggccggat aagacgcgac cggcgtcgca tccggcgcta gccgtaaatt ctatacaaaa
    70201 ttaccgccgc tccagaatct caaagcaata gctgtgagag ttctgcgcat cagcatcgtg
    70261 gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc
    70321 ttccacttct gcgtcgatat gcgtcagata cagtttttgc gcttttggca agaactgttc
    70381 ataaacgcga ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc
    70441 ttcatccacc gacttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat
    70501 aatatttttg cgtcctggca acggacgacc gattgattcc caggtatggc ggcccataat
    70561 cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg
    70621 catggcgttt tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat
    70681 tgagatttcc cgataaaaaa aattgtcgcc actatacgta aagcgtaaac cgtcgtcgac
    70741 tggtgcgagg atgatgttga ggaaaatttt atattctgct ggcgagtcca cgctctctcc
    70801 ctggactcgc cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag tgtgatttac
    70861 gtcactcttt attaggatga gggtttcgtt tccggttcat ccgccatgtt gccggtatgt
    70921 ttaccttctt cggttccctg ccagccatgt cgttgaatta atgacagatg ttcgcggtcc
    70981 tcggtaatga tctcacttaa catcgcgctg gtgcgtttat agaccgccgc gcgggctttg
    71041 gtgtcgttct caaccattgc catctcttcc accatctgaa tattaaagcg gcggaacaca
    71101 tcggcacgtt ctcgcgcttc atacggcccc agacctaaac tttccagtgc cagacgcccg
    71161 gttttcagcg caccttcgaa ggtttcacgc tccggctttt caacgcctgc ctgacgcaaa
    71221 cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg gaaatgttct
    71281 ttcaccatct ctgtcagttg caggttggtt tgcggatcgt cgatggcgtt aatcagcact
    71341 tccgctttcg ccgctccggc agattccagt aaatccatcc gcgtggcatc gccataaaac
    71401 actttcatac caaatttacg caaggtttcg atatggtccg gatcgtgatc gagtaccacc
    71461 attttcaccc cgctggagag cagtaaacgt ccggtaatct gcccaaaacg accgaatccg
    71521 gcgataatca cgcgcggctg ttcttcgtcg atctcatcgg cttcacgcgc ttcctcagta
    71581 gaagattgct caaggcgatt gaggatcacc agcagaatcg gcgttgctgc catcgacagc
    71641 gccaccgcca gggtcagcga tttcgcccac tccggctcca gcacattcgc catctgcgcc
    71701 gcgccaaata ccacaaaggc aaactcactg ccctgcccta acaacaccgc aaaccaacga
    71761 cgctgtttat ttggcacttg caacggtcgg gcaatcagcc acagcatggc gattttgatg
    71821 atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt tttcaagcag cgtgccaaag
    71881 tctatcgaca tgccaacacc gatgaaaaac agccccaaca gcaaaccttt aaatggttcg
    71941 atatcgctct ccagcgcatg acggtattcc gagcttgcca gcagtacgcc cgccagaaac
    72001 gcgcccatcg ccatcgacaa gccgacctct tccagcagca aaccaaagcc aaacacgagg
    72061 aataacgcca cggcactaaa cacttcccgc aagccagagc gggctacaaa acgcagcgcc
    72121 ggacgcgtga catagcgccc cagcaatacc accagcacca gcgcacccgc cacttttaac
    72181 gccgagagag caaatgcgcc catcgtcgtc gaggcactgc tcgttgccag tagcggaatc
    72241 atcgccacca gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa ggcactgcga
    72301 cccatttgcg tcaccatcag attgcgttca ttcatcgcct gcatggcaat cgccgtagag
    72361 gagagcgcca gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc aagtaacatg
    72421 cagaacagcc ccagcaggcc gccgcaaatc accatctgca atgcgccacc gccgaacact
    72481 gccgcacgca gcttccacag cctttgtgga tcgagttcga ggccgataat aaacagcatc
    72541 agcaccaccc caatctcggc aaagtgcaga atagattcgg catcggtcac cagtcgcagc
    72601 ccccacgggc caataatgca gccggcgatc aggtagccaa gtaccgatcc cagaccaaga
    72661 cgtaccgcaa tgggtacaat cagcgctgcc gaaccgagat aaatcagcgc ctgaatcagc
    72721 gtatggctat ccatgatggg cctcctgcca ttccagcaga cgttgcttat agtgacgcgc
    72781 ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag
    72841 ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc
    72901 cggatgcgca ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg cccacagcaa
    72961 atgtttgcca tgcagcgcct tgccgccgtg accgtaagcc cagccgtgcg acaaaacttt
    73021 atcgatccaa agtttaagaa gcggaggaat gctgtaccac tgcatcggat gctgccagac
    73081 gatcagatcg gcgcgagaca gcgcctcctg ctcggcggca atatcgatat tgaagtcagg
    73141 atagagttga taaagagagc gaatttcgac gccttccagc gtccttgcct gttcaagcat
    73201 ccgtttattc gcatgggaat gatgcggata cggatgcgca taaattataa gaatcatagg
    73261 tagcccgcgt gattacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    73321 atagtcaata atattgattc agaaaagaga gaaatctgaa caatttaccc cattctctgc
    73381 ccgcggatcg tcatattgct ggtctgcgcc agtgacatcc ctcgagtctc cggggcaaac
    73441 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    73501 ataccgtaat tattgataaa gatcggtagt gcccaggtcg aaacaatggt gccaatacga
    73561 cttaaagaca taatcacgcc cacggcagag gcgcggatat ctgtcgggaa gagttcatta
    73621 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    73681 atcactacca gccagatccc catatccggg atcagcccca aaaccgccag cgccagcgtc
    73741 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc
    73801 ggcggaatac agccgagcat aaagaacagg ctaatcacca cgtttcccag cgccgcgttt
    73861 ttgccaaccc ccaatcccaa caaaccaacg atttgcgggc caaaggtgta aatggcgaac
    73921 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    73981 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    74041 tcgccaaaca gtttgatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    74101 cagcggggag attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca ggggatcgcc
    74161 gcgctaccca gcatccagcg ccagccgcct tccacatcat aaagccagta gccgaccaga
    74221 tcggcacagg tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc
    74281 tgacgggtac tggagaactc ggtgatcatt gaggtggcga tgggataatc tgcaccgatg
    74341 acaatgccga taagtacccg catcaccaac agttcgacgg gggatgaaac aaacatcgtc
    74401 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    74461 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    74521 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gggtcagttg ctccagcgcc
    74581 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    74641 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    74701 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    74761 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    74821 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    74881 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcgc
    74941 ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    75001 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    75061 atgccatcct tgattagatt gatgaagccc actttcttac cgtggcggag gattttcttg
    75121 cgcatcagtt ctggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    75181 agttccgggt agccgctaaa catgcgtggg ttatcaagga acgccggtag tttctgatac
    75241 atacgcatat cgcgcagcgg accactctca agatgctgac gatattccgc cagtttttgc
    75301 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcggc ttccccggcg
    75361 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    75421 tcaccggcaa tcaatacgcc gtcaccaacc aactccggca gcatgttgat gcctgcttcc
    75481 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaactgcc
    75541 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga
    75601 tgcagaccac aaaccagccc cagcgacagg gtgttttcat tggtataaag aaaaccgccg
    75661 cccatcaggc catcggtggg tgatcccgca aacaggcaag ccgccccctg attaccctgc
    75721 aactgaaaac ggtcttcaat aaccgacttc ggtaactcga tcagttcctt cacgccaacc
    75781 gccacatccg tcggtttgac gcgttttgcc atccccaatt tttcggcaag gatggagttc
    75841 accccatcag caaggatcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg
    75901 acgactttgc catcgcgctg tacgaggtta tcgacacgga tcccggtaat taactgcgcg
    75961 cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa
    76021 gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt
    76081 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggattcggcg
    76141 aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg
    76201 gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc
    76261 agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg
    76321 gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac
    76381 gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat
    76441 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgatg
    76501 cattagcacc aaccatgtgc tggatctgcc cggagatccc caccgccaga tacaattcag
    76561 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    76621 ccgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    76681 gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gactaccaga cgggctttgt
    76741 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    76801 gagcctgcca ctccacggtg tgcgtttcac cagtgcgtga cgcgtctggc tgagccgcat
    76861 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    76921 tcgccagacc gccgtaaacc atgtgtttca ctgtcgcttt accgtcctgt acgctgacgg
    76981 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    77041 tgccgcgacg ggtgtttggc aacagcacca gaccgtctgc gccgtgctgg cgaatagtgt
    77101 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    77161 agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg
    77221 cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaagggg
    77281 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    77341 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    77401 gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tctgaccagg ctgcctctgc
    77461 gttaaaaccg atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    77521 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    77581 cagcggaatg cttaacgttt ctgtttcatc ttccagttcg cgctcaacgg tgagggtatc
    77641 tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    77701 taccagcaaa ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcaa
    77761 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    77821 ctgctcgaac tggtcatcaa ttaccacaat cagttcatcc gggccgcgcg ataacacatc
    77881 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc
    77941 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct
    78001 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    78061 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    78121 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcactcc
//
LOCUS       DAZFZC010000010        74135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFZC010000010 DAZFZC010000000
VERSION     DAZFZC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.010"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..386
                     /gene="cmtB"
                     /locus_tag="ACHKXP_000795"
     CDS             3..386
                     /gene="cmtB"
                     /locus_tag="ACHKXP_000795"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO7645864.1"
                     /translation="MSVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGP
                     YYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIG
                     AIQALSELLCEEEILEQLLTASSEK"
     gene            441..1829
                     /gene="cmtA"
                     /locus_tag="ACHKXP_000796"
     CDS             441..1829
                     /gene="cmtA"
                     /locus_tag="ACHKXP_000796"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO7645865.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1844..3121
                     /gene="yggP"
                     /locus_tag="ACHKXP_000797"
     CDS             1844..3121
                     /gene="yggP"
                     /locus_tag="ACHKXP_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7645866.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3118..4083
                     /gene="glpX"
                     /locus_tag="ACHKXP_000798"
     CDS             3118..4083
                     /gene="glpX"
                     /locus_tag="ACHKXP_000798"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7645867.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDDLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVSASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4105..4614
                     /gene="fumE"
                     /locus_tag="ACHKXP_000799"
     CDS             4105..4614
                     /gene="fumE"
                     /locus_tag="ACHKXP_000799"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO7645868.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4611..5324
                     /gene="yggC"
                     /locus_tag="ACHKXP_000800"
     CDS             4611..5324
                     /gene="yggC"
                     /locus_tag="ACHKXP_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO7645869.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLTENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLTRQVAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            5609..6628
                     /gene="epd"
                     /locus_tag="ACHKXP_000801"
     CDS             5609..6628
                     /gene="epd"
                     /locus_tag="ACHKXP_000801"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO7645870.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6678..7841
                     /gene="pgk"
                     /locus_tag="ACHKXP_000802"
     CDS             6678..7841
                     /gene="pgk"
                     /locus_tag="ACHKXP_000802"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO7645871.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8056..9135
                     /gene="fbaA"
                     /locus_tag="ACHKXP_000803"
     CDS             8056..9135
                     /gene="fbaA"
                     /locus_tag="ACHKXP_000803"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO7645872.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(9199..9297)
                     /locus_tag="ACHKXP_000804"
                     /pseudo
     CDS             complement(9199..9297)
                     /locus_tag="ACHKXP_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9493..10353
                     /gene="mscS"
                     /locus_tag="ACHKXP_000805"
     CDS             9493..10353
                     /gene="mscS"
                     /locus_tag="ACHKXP_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO7645873.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            10492..11127
                     /gene="argO"
                     /locus_tag="ACHKXP_000806"
     CDS             10492..11127
                     /gene="argO"
                     /locus_tag="ACHKXP_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO7645874.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VMKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLALLAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11220..11960
                     /gene="yggE"
                     /locus_tag="ACHKXP_000807"
     CDS             11220..11960
                     /gene="yggE"
                     /locus_tag="ACHKXP_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO7645875.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12127..13023
                     /gene="ygfI"
                     /locus_tag="ACHKXP_000808"
     CDS             12127..13023
                     /gene="ygfI"
                     /locus_tag="ACHKXP_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7645876.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(13020..14498)
                     /gene="scpC"
                     /locus_tag="ACHKXP_000809"
     CDS             complement(13020..14498)
                     /gene="scpC"
                     /locus_tag="ACHKXP_000809"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO7645877.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     VIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(14522..15307)
                     /gene="scpB"
                     /locus_tag="ACHKXP_000810"
     CDS             complement(14522..15307)
                     /gene="scpB"
                     /locus_tag="ACHKXP_000810"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO7645878.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15318..16313)
                     /gene="meaB"
                     /locus_tag="ACHKXP_000811"
     CDS             complement(15318..16313)
                     /gene="meaB"
                     /locus_tag="ACHKXP_000811"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO7645879.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEDFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16306..18450)
                     /gene="scpA"
                     /locus_tag="ACHKXP_000812"
     CDS             complement(16306..18450)
                     /gene="scpA"
                     /locus_tag="ACHKXP_000812"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO7645880.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVHQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVYVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(18654..19547)
                     /gene="argP"
                     /locus_tag="ACHKXP_000813"
     CDS             complement(18654..19547)
                     /gene="argP"
                     /locus_tag="ACHKXP_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO7645881.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19689..19919
                     /locus_tag="ACHKXP_000814"
     CDS             19689..19919
                     /locus_tag="ACHKXP_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645882.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLVNGELVRLFAPWSGIP
                     RLLHALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            19975..20634
                     /gene="rpiA"
                     /locus_tag="ACHKXP_000815"
     CDS             19975..20634
                     /gene="rpiA"
                     /locus_tag="ACHKXP_000815"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO7645883.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20890..22122
                     /gene="serA"
                     /locus_tag="ACHKXP_000816"
     CDS             20890..22122
                     /gene="serA"
                     /locus_tag="ACHKXP_000816"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO7645884.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22351..22410
                     /gene="ibsC"
                     /locus_tag="ACHKXP_000817"
     CDS             22351..22410
                     /gene="ibsC"
                     /locus_tag="ACHKXP_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO7645885.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(22511..23113)
                     /gene="fau"
                     /locus_tag="ACHKXP_000818"
     CDS             complement(22511..23113)
                     /gene="fau"
                     /locus_tag="ACHKXP_000818"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO7645886.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(23134..23317)
                     /gene="ssrS"
                     /locus_tag="ACHKXP_000819"
     ncRNA           complement(23134..23317)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKXP_000819"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23359..23688)
                     /gene="zapA"
                     /locus_tag="ACHKXP_000820"
     CDS             complement(23359..23688)
                     /gene="zapA"
                     /locus_tag="ACHKXP_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7645887.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23856..24434
                     /gene="ygfB"
                     /locus_tag="ACHKXP_000821"
     CDS             23856..24434
                     /gene="ygfB"
                     /locus_tag="ACHKXP_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO7645888.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHLQPTAPEVQKPTLH"
     gene            24460..25785
                     /gene="pepP"
                     /locus_tag="ACHKXP_000822"
     CDS             24460..25785
                     /gene="pepP"
                     /locus_tag="ACHKXP_000822"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO7645889.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMVAARKQ"
     gene            25782..26960
                     /gene="ubiH"
                     /locus_tag="ACHKXP_000823"
                     /gene_synonym="visB"
     CDS             25782..26960
                     /gene="ubiH"
                     /locus_tag="ACHKXP_000823"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO7645890.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATATESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLYRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            26983..28185
                     /gene="ubiI"
                     /locus_tag="ACHKXP_000824"
                     /gene_synonym="visC"
     CDS             26983..28185
                     /gene="ubiI"
                     /locus_tag="ACHKXP_000824"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO7645891.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVQEPLAANA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFASHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28259..28342)
                     /locus_tag="ACHKXP_000825"
     CDS             complement(28259..28342)
                     /locus_tag="ACHKXP_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645892.1"
                     /translation="MSKNTKSKNNGIRKYNAKTEVKLVYFK"
     gene            28633..29727
                     /gene="gcvT"
                     /locus_tag="ACHKXP_000826"
     CDS             28633..29727
                     /gene="gcvT"
                     /locus_tag="ACHKXP_000826"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO7645893.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            29751..30140
                     /gene="gcvH"
                     /locus_tag="ACHKXP_000827"
     CDS             29751..30140
                     /gene="gcvH"
                     /locus_tag="ACHKXP_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO7645894.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASGESELESLLDATAYEALLEDE"
     gene            30259..33132
                     /gene="gcvP"
                     /locus_tag="ACHKXP_000828"
     CDS             30259..33132
                     /gene="gcvP"
                     /locus_tag="ACHKXP_000828"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO7645895.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFDGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            33398..34141
                     /gene="ygfF"
                     /locus_tag="ACHKXP_000829"
     CDS             33398..34141
                     /gene="ygfF"
                     /locus_tag="ACHKXP_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7645896.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(34198..35631)
                     /gene="bglA"
                     /locus_tag="ACHKXP_000830"
     CDS             complement(34198..35631)
                     /gene="bglA"
                     /locus_tag="ACHKXP_000830"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO7645897.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            35676..35987
                     /gene="yqfB"
                     /locus_tag="ACHKXP_000831"
     CDS             35676..35987
                     /gene="yqfB"
                     /locus_tag="ACHKXP_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO7645898.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            36151..36810
                     /gene="yqfA"
                     /locus_tag="ACHKXP_000832"
     CDS             36151..36810
                     /gene="yqfA"
                     /locus_tag="ACHKXP_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO7645899.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(37006..37986)
                     /gene="ygfZ"
                     /locus_tag="ACHKXP_000833"
     CDS             complement(37006..37986)
                     /gene="ygfZ"
                     /locus_tag="ACHKXP_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO7645900.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            38229..38495
                     /gene="sdhE"
                     /locus_tag="ACHKXP_000834"
     CDS             38229..38495
                     /gene="sdhE"
                     /locus_tag="ACHKXP_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO7645901.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            38476..38883
                     /gene="ygfX"
                     /locus_tag="ACHKXP_000835"
     CDS             38476..38883
                     /gene="ygfX"
                     /locus_tag="ACHKXP_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO7645902.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(38923..39444)
                     /gene="fldB"
                     /locus_tag="ACHKXP_000836"
     CDS             complement(38923..39444)
                     /gene="fldB"
                     /locus_tag="ACHKXP_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO7645903.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            39556..40452
                     /gene="xerD"
                     /locus_tag="ACHKXP_000837"
     CDS             39556..40452
                     /gene="xerD"
                     /locus_tag="ACHKXP_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO7645904.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            40477..41187
                     /gene="dsbC"
                     /locus_tag="ACHKXP_000838"
     CDS             40477..41187
                     /gene="dsbC"
                     /locus_tag="ACHKXP_000838"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO7645905.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            41193..42926
                     /gene="recJ"
                     /locus_tag="ACHKXP_000839"
     CDS             41193..42926
                     /gene="recJ"
                     /locus_tag="ACHKXP_000839"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO7645906.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            43017..44115
                     /gene="prfB"
                     /locus_tag="ACHKXP_000840"
     CDS             join(43017..43091,43093..44115)
                     /gene="prfB"
                     /locus_tag="ACHKXP_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO7645907.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            44125..45642
                     /gene="lysS"
                     /locus_tag="ACHKXP_000841"
     CDS             44125..45642
                     /gene="lysS"
                     /locus_tag="ACHKXP_000841"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7645908.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(45685..46233)
                     /gene="idi"
                     /locus_tag="ACHKXP_000842"
     CDS             complement(45685..46233)
                     /gene="idi"
                     /locus_tag="ACHKXP_000842"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO7645909.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            46288..46359
                     /locus_tag="ACHKXP_000843"
     CDS             46288..46359
                     /locus_tag="ACHKXP_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7645910.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(46356..46481)
                     /gene="yqfG"
                     /locus_tag="ACHKXP_000844"
     CDS             complement(46356..46481)
                     /gene="yqfG"
                     /locus_tag="ACHKXP_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO7645911.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(46483..47931)
                     /gene="uacT"
                     /locus_tag="ACHKXP_000845"
     CDS             complement(46483..47931)
                     /gene="uacT"
                     /locus_tag="ACHKXP_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO7645912.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            48367..50286
                     /gene="ygfT"
                     /locus_tag="ACHKXP_000846"
     CDS             48367..50286
                     /gene="ygfT"
                     /locus_tag="ACHKXP_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO7645913.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWCPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            50286..50774
                     /gene="ygfS"
                     /locus_tag="ACHKXP_000847"
     CDS             50286..50774
                     /gene="ygfS"
                     /locus_tag="ACHKXP_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7645914.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(50810..52177)
                     /gene="ghxQ"
                     /locus_tag="ACHKXP_000848"
                     /gene_synonym="ygfQ"
     CDS             complement(50810..52177)
                     /gene="ghxQ"
                     /locus_tag="ACHKXP_000848"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO7645915.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(52213..53529)
                     /gene="guaD"
                     /locus_tag="ACHKXP_000849"
     CDS             complement(52213..53529)
                     /gene="guaD"
                     /locus_tag="ACHKXP_000849"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO7645916.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(53547..54947)
                     /gene="xanQ"
                     /locus_tag="ACHKXP_000850"
     CDS             complement(53547..54947)
                     /gene="xanQ"
                     /locus_tag="ACHKXP_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO7645917.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(55112..57982)
                     /gene="xdhD"
                     /locus_tag="ACHKXP_000851"
     CDS             complement(55112..57982)
                     /gene="xdhD"
                     /locus_tag="ACHKXP_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO7645918.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(57979..58758)
                     /gene="ygfM"
                     /locus_tag="ACHKXP_000852"
     CDS             complement(57979..58758)
                     /gene="ygfM"
                     /locus_tag="ACHKXP_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO7645919.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(58809..60137)
                     /gene="ssnA"
                     /locus_tag="ACHKXP_000853"
     CDS             complement(58809..60137)
                     /gene="ssnA"
                     /locus_tag="ACHKXP_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO7645920.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(60140..63238)
                     /gene="ygfK"
                     /locus_tag="ACHKXP_000854"
     CDS             complement(60140..63238)
                     /gene="ygfK"
                     /locus_tag="ACHKXP_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO7645921.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDICGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTIEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLLVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(63560..64138)
                     /gene="mocA"
                     /locus_tag="ACHKXP_000855"
     CDS             complement(63560..64138)
                     /gene="mocA"
                     /locus_tag="ACHKXP_000855"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO7645922.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            64241..65011
                     /gene="yqeC"
                     /locus_tag="ACHKXP_000856"
     CDS             64241..65011
                     /gene="yqeC"
                     /locus_tag="ACHKXP_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO7645923.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            65059..66684
                     /gene="yqeB"
                     /locus_tag="ACHKXP_000857"
     CDS             65059..66684
                     /gene="yqeB"
                     /locus_tag="ACHKXP_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO7645924.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(66905..67837)
                     /gene="arcC"
                     /locus_tag="ACHKXP_000858"
     CDS             complement(66905..67837)
                     /gene="arcC"
                     /locus_tag="ACHKXP_000858"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7645925.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(67885..69270)
                     /gene="hyuA"
                     /locus_tag="ACHKXP_000859"
     CDS             complement(67885..69270)
                     /gene="hyuA"
                     /locus_tag="ACHKXP_000859"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO7645926.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(69323..70534)
                     /gene="ygeY"
                     /locus_tag="ACHKXP_000860"
     CDS             complement(69323..70534)
                     /gene="ygeY"
                     /locus_tag="ACHKXP_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO7645927.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(70592..71788)
                     /gene="dpaL"
                     /locus_tag="ACHKXP_000861"
     CDS             complement(70592..71788)
                     /gene="dpaL"
                     /locus_tag="ACHKXP_000861"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO7645928.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAISEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(71846..73033)
                     /gene="ygeW"
                     /locus_tag="ACHKXP_000862"
     CDS             complement(71846..73033)
                     /gene="ygeW"
                     /locus_tag="ACHKXP_000862"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO7645929.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            73512..>74135
                     /locus_tag="ACHKXP_000863"
     CDS             73512..>74135
                     /locus_tag="ACHKXP_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFO7645930.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPIRFR"
BASE COUNT        17917 a        18420 c        19646 g        18152 t
ORIGIN      
        1 caatctcagt tatacattca gcaaaagatt ggcaggaagc tatcgatttc tcgatggtat
       61 cattgctgga taaaaactat atcagcgaga attacattca ggcaattaaa gattccacca
      121 ttaacaatgg cccttattat attctcgcac caggcgtggc aatgcctcat gcgcgaccgg
      181 aatgtggggc gcttaaaacc gggatgtctt tgacattact tgaacaaggt gtttattttc
      241 cggggaatga cgaaccaatt aaattactca tcggactctc tgctgccgat gccgattcgc
      301 acattggcgc tattcaggcg ttaagtgaat tactgtgcga agaagaaata ctcgaacaac
      361 tcttaacagc atcatcagaa aaataattag cggacattat cagccgcgga taattactct
      421 ctcctctaca ggataatatt atggaaaaca agtctgctcg tgcaaaggtc caggcttttg
      481 ggggcttttt gactgcaatg gtcatcccca atattggtgc ttttattgcc tggggtttta
      541 ttactgcgtt atttattccc accggttggc tgcctaacga acatttcgcc aaaattgtcg
      601 gcccgatgat tacctattta ttgcccgtga tgattggttc tacaggtggt catctggtcg
      661 gcggtaaacg cggggcggtc atgggcggaa taggtactat tggtgtgatc gttggcgcag
      721 agatcccgat gttccttggc tcaatgatca tggggccgct cggtgggttg gtcataaaat
      781 atgtcgataa ggcactggaa aaacgcatac ctgccggttt tgagatggtt atcaataact
      841 tctcattagg tatcgcgggg atgctccttt gtctgctggg ttttgaagtt atcggcccgg
      901 cggtgttaat tgccaatact ttcgtcaaag agtgtattga ggcgctggta catgcgggtt
      961 atctgccatt gttgtcagtc atcaatgaac cggcgaaagt gcttttcctc aataatgcga
     1021 tcgatcaggg cgtctattat ccgctgggaa tgcaacaggc ttcggttaac ggtaaatcca
     1081 tcttctttat ggtggcctct aacccaggtc cgggcctggg gctgctgctg gcgtttacct
     1141 tgtttggtaa agggatgagt aaacgttctg cgcccggggc gatgattatt cacttcctcg
     1201 gtgggatcca cgaactgtat ttcccatatg tgctgatgaa gccgctgact attattgcca
     1261 tgattgcggg cggtatgtct ggtacctgga tgtttaactt actggacggt ggtctggtgg
     1321 ctggcccaag tccggggtct atctttgctt acctggcact gacgccgaaa ggctcgttcc
     1381 tggcgacaat tgccggtgtt acggtaggta ccctggtgtc ctttgctatc acttcgctga
     1441 tactgaagat ggaaaaaacg gtggaaacgg agagcgaaga tgagtttgct cagtcagcca
     1501 atgcggttaa ggcgatgaaa caagagggtg cattctcgtt aagcagggtt aagcgtatcg
     1561 cctttgtttg cgatgcgggg atgggctcca gtgcgatggg cgcgaccacc ttccgtaaac
     1621 gcctggaaaa agcggggctg gcaattgaag taaaacatta cgccatagaa aacgtgcctg
     1681 cggatgcgga tatcgtcgtt actcatgcca gtctggaagg gcgcgtgaaa cgtgtgacgg
     1741 ataaaccact gatattgatt aataactata ttggcgatcc aaaactcgac actttattta
     1801 atcaattaac cgccgaacat aaacactgat tacagaggta aaaatgaaaa ccaaagttgc
     1861 tgctatttat ggcaagcggg atgtccgtct gcgcgaattt gaactgccag aaattaccga
     1921 taatgaatta ctggtgagtg tgatttctga cagcgtctgt ttatccacct ggaaagcggc
     1981 gttactcggt agtgaacata aacgcgtacc cgacgattta gaaaatcatc cggtcattac
     2041 cgggcatgaa tgtgccgggg ttattgtcga agtgggtaaa aatctcactg gcaaatataa
     2101 aaaaggccag cgttttgtat tgcaaccggc gatggggtta ccaagtggat attcagcggg
     2161 ctacagctac gaatattttg gcggcaatgc cacttatatg attattcccg aaatcgccat
     2221 taatttgggc tgcgtattac cgtatcacgg ctcttatttt gctgcggcgt cgctggcaga
     2281 gcctatgtgc tgcattattg gtgcttatca tgccaattat cacaccacgc aatatgttta
     2341 tgagcatcgc atgggcgtca aacctggcgg caatattgca ctgctggcgt gtgcaggtcc
     2401 gatgggcatt ggcgctatcg attacgccat taacggcggc atacaaccgt cgcgggtggt
     2461 ggtggtcgat atcgacgaca aacgtctggc gcaggtacag aagctgctgc cggtggaact
     2521 ggcggccagt aaaggcattg agctggtgta tgtgaatacc aaagggatga gcgatcctgt
     2581 ccagatgctg cgggcgctga caggagatgt cgggttcgat gacatttttg tttatgcggc
     2641 ggtgcctgct gtcgttgaga tggctgacga attactggcg gaagatggct gtctgaactt
     2701 ctttgccggt ccgacggata aaaacttcaa agtaccgttt aatttctaca acgtccatta
     2761 caacagcacg cacgtcgtcg gtacatccgg cggttcaacg gacgacatga aagaggcgat
     2821 tgcccttagc gccactgggc agttacagcc gtcgtttatg gtgacccata tcggtggcct
     2881 ggatgcggtg ccagaaaccg tgctcaatct gccggatatc cctggcggta aaaaactcat
     2941 ttataacggc gtgaccatgc cgctcactgc cattgccgat tttgccgaaa aaggcaaaac
     3001 cgatccgctg tttaaagagt tggcgcggct ggttgaggaa acgcacggca tctggaatga
     3061 acaggccgag aaatatctgc tggcacaatt tggcgttgat atcggggagg ccgcgcaatg
     3121 atgtccctgg cgtggccatt atttcgcgtt acggaacagg cagcgttggc tgcctggccg
     3181 caaaccggat gtggcgacaa aaataaaatt gatgacctgg cggtcaccgc gatgcgccag
     3241 gcattaaacg acgtcgcttt tcgtggacga gtggttatcg gcgaaggaga aattgaccat
     3301 gcgccgatgc tgtggattgg cgaagaggtg ggcaaaggcg atgggccaga agtcgatatc
     3361 gcggttgatc ccattgaagg cacgcggatg gtggcgatgg ggcagagcaa tgcgctggcg
     3421 gtcatggctt tcgcccctcg agatagcctg ttacatgcgc ccgatatgta tatgaaaaag
     3481 ctggttgtta atcgactggc tgccggggtg attgatctgt ctttgcctct ggcggacaac
     3541 ttgcgtaacg tggcgcgggc gttaggtaaa ccgctggaca agctgcgcat ggttacgctg
     3601 gataaaccgc gcctgagtgc cgcgattgaa gaagcaacac agctgggcgt gaaggttttt
     3661 gccctgccgg atggcgatgt ctccgccagc gtgctgacct gctggcagga taatccttac
     3721 gatgtgatgt acaccatcgg cggtgcgcca gaaggcgtga tttctgcctg tgccgttaaa
     3781 gcattaggcg gggatatgca ggccgagctg attgactttt gtcaggctaa aggggattac
     3841 acggaaaatc ggcagattgc agagcaggag cgcaagcgtt gcaaggcaat gggcgttgac
     3901 gttaaccgtg tgtactcgct cgatgaactg gtgaggggga acgatatcct ctttagcgct
     3961 acgggcgtga cgggcggtga gctggtgaac ggtatccaac agacggcgaa tggggtgcgg
     4021 acgcagacat tactgatcgg cggcgcggac caaacgtgta atataataga ctccctgcat
     4081 tgatggtgat ctatcggaag accaatggcg acgctgacag aagatgatgt gcttgagcaa
     4141 ctggatgcac aggacaattt attttcattt atgaaaactg cgcataccat tttgctccag
     4201 gggatacgcc agtttctgcc gtcgctgttt gtcgataacg atgaagagat cgtggaatat
     4261 gcagtgaagc cgttactcgc ccaaagcggc ccgcttgacg atattgatgt tgcactgcgt
     4321 ttgatttatg cgttggggaa aatggataaa tggttgtacg ccgatatcac gcatttttcc
     4381 cagttctggc attacctgaa cgaacaggat gaaacgcccg gatttgccga tgacatgacc
     4441 tgggatttta tcagcaacgt caatagcata acctgcaatg cgacgctcta tgacgcgttg
     4501 aaagcgatga agttcgccga tttcgccgtc tggtcagagg cgcgttttag cggaatggtc
     4561 aaaacggcgc tgacgctggc agtaacgacc accttaaagg aattaacgcc gtgaaaattg
     4621 aattaacggt gaatgggctg aagatacagg cacagtacca gaacgaagag attgaaaatg
     4681 tacataaacc gttgctgcac atgttggcgg ccttacagac ggtaaatccg cagcggcgta
     4741 cggtggtttt tctttgcgct ccgccgggga cgggtaaatc aaccctaacc accttctggg
     4801 aatatctcgc gcaacaagac ccggaactgc ccgctattca gacgctcccg atggatggtt
     4861 ttcaccatta caatagctgg ctggatgcgc atcaattgcg ccccttcaaa ggcgcaccag
     4921 agacatttga cgttgcgaaa ctgacggaaa atctgcgcca ggtcgtggaa ggggattgta
     4981 cgtggccgca gtacgatcga caaaagcatg atcctgttga agatgcgttg cacgttaccg
     5041 caccactcgt catcgtcgaa ggaaactggt tgttactgga tgatgaaaag tggctggagt
     5101 tggcatcttt ctgcgatttt tctattttta ttcatgcacc tgctcaaatt ctgcgggagc
     5161 gcttgattag tcgtaaaatt gcgggaggat taacgcggca agtagccgaa gcattttatg
     5221 cccgcaccga tggccccaat gtcgagcgtg tgctgatgaa tagccgtcag gccaatctga
     5281 tagtagagat gactgaagaa ggccggtatc acttcacaag ctgacaattt attccagtta
     5341 ttgtagggtt attattacgc cagcctggaa taaagcttcc cacaaaaggg gataaaagtg
     5401 tgatgtgagt cagataaatg tcttcttcgg ctggacaaac attcctttta ttccacgttt
     5461 cgcttatcct agctgaagcg tttcagtcga ttaaatgttc gacaattaac caatcagtcg
     5521 cagtttgcga caggtaaggt ttccccggac gatttgctgg attactctgt ctggcaaatt
     5581 tgctcaggga aaaccttgca ggagatctat gaccgtacgc gtagcgataa atggcttcgg
     5641 tcgcatcggg cgtaatgtgg ttcgtgcttt gtatgaatcc ggacgccggg cggaaattac
     5701 cgtggtggca atcaacgaac tggcggatgc tgcgggcatg gcgcatttgt tgaaatatga
     5761 caccagccat ggccgttttg catgggaagt acgacaggaa cgcgatcaac tttttgttgg
     5821 tgatgacgcc atccgcgtat tgcatgaacg ttcactgcaa tcgctcccct ggcgtgaact
     5881 tggcgttgat gtagtcctcg actgcaccgg cgtatatggc tcccgcgagc atggcgaagc
     5941 gcatattgcc gccggggcca aaaaagtgct cttttcacat cctggcagta acgatctcga
     6001 cgcgaccgtt gtttacggcg tcaatcagga tcaacttcgt gcggaacacc gcatcgtttc
     6061 taacgcttcc tgtaccacga attgcataat tcccgtcatc aaattgttag atgatgcgta
     6121 cggtattgag tccggcactg tgaccacaat tcactccgcc atgcacgatc aacaggttat
     6181 tgatgcatac catcctgacc tgcgtcgcac ccgggcagcc agccagtcga tcattccggt
     6241 cgatactaaa ctggccgccg gtatcacacg attttttccg caatttaacg atcgctttga
     6301 agcgattgcg gtacgtgtgc caaccataaa tgtgacggca atcgatttaa gcgtgacggt
     6361 gaagaaacct gtaaaagcca atgaagtcaa cctgttgctg caaaaagcag cacaaggtgc
     6421 atttcatggt atagttgact atacggaatt gccgttggtc tctgtagatt ttaaccacga
     6481 tccgcacagt gccattgtcg atggcaccca aacccgggtc agtggcgctc acctgatcaa
     6541 aacgttggtc tggtgcgata acgaatgggg ctttgctaac cgaatgctcg acacgacgtt
     6601 agctatggct actgttgctt tcaggtaaga cgcaagcagc gtctgcaaaa cttttagaat
     6661 caacgagagg attcaccatg tctgtaatta agatgaccga tctggatctt gctgggaaac
     6721 gtgtatttat ccgtgcggat ctgaacgtac cagtaaaaga cgggaaagta accagcgacg
     6781 cgcgtatccg tgcttctctg ccgaccatcg aactggccct gaaacaaggc gcaaaagtga
     6841 tggtaacttc ccacctgggt cgtcctaccg aaggcgagta caacgaagaa ttctctctgc
     6901 tgccggttgt taactacctg aaagacaaac tgtctaaccc ggttcgtctg gttaaagatt
     6961 acctcgacgg cgttgacgtt gctgaaggtg aactggttgt tctggaaaac gttcgcttca
     7021 acaaaggcga gaagaaagac gacgaaaccc tgtccaaaaa atacgctgca ctgtgtgacg
     7081 tgttcgtaat ggacgcattc ggtactgctc accgcgcgca ggcttctact cacggtatcg
     7141 gtaaattcgc tgacgttgcg tgcgcaggcc cgctgctggc agctgaactg gacgcgctgg
     7201 gtaaagcact gaaagaacct gctcgcccga tggtggctat cgttggtggt tctaaagtat
     7261 ctaccaaact gaccgttctg gactccctgt ctaaaatcgc tgaccagctg attgttggtg
     7321 gtggtatcgc taacaccttt atcgcggcac aaggccacga tgtgggtaaa tccctgtacg
     7381 aagctgacct ggttgacgaa gctaaacgtc tgctgaccac ctgcaacatc ccggttccgt
     7441 ctgatgttcg cgtagcaacc gagttctctg aaactgcacc ggctaccctg aaatctgtta
     7501 acgatgtgaa agctgacgag cagatcctgg atatcggtga tgcttccgct caggaactgg
     7561 ctgaaatcct gaagaatgcg aaaaccattc tgtggaacgg tccggttggc gtgttcgaat
     7621 tcccgaactt ccgcaaaggt actgaaatcg tggctaacgc tatcgcagac agcgaagcgt
     7681 tctccatcgc tggcggcggc gacactctgg cagcaatcga cctgttcggc attgctgaca
     7741 aaatctccta catctccact ggcggcggcg cattcctcga attcgtggaa ggtaaagtac
     7801 tgcctgcagt agcgatgctc gaagagcgcg ctaagaagta aaaaatcaca gggcagggaa
     7861 acctgccctt gtttcagcgc gcttttagag cacgcaattt ttcatactct aaataattcg
     7921 agttgcagga aggcgacaag agagtgaatc cccaggagct tacataagta agtgactggg
     7981 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtataa ggcccgacga
     8041 tacaggacaa gagacatgtc taagattttt gatttcgtaa aacctggcgt aatcactggt
     8101 gatgacgtac agaaagtttt ccaggtagca aaagaaaaca acttcgcact gccagcagta
     8161 aactgcgtcg gtactgactc catcaacgcc gtactggaaa ccgctgctaa agttaaagcg
     8221 ccggttatcg ttcagttctc caacggtggt gcttccttta tcgctggtaa aggcgtgaaa
     8281 tctgacgttc cgcagggtgc tgctatcctg ggcgcgatct ctggtgcgca tcacgttcac
     8341 cagatggctg aacattatgg tgttccggtt atcctgcaca ctgaccactg cgcgaagaaa
     8401 ctgctgccgt ggatcgacgg tctgttggac gcgggtgaaa aacacttcgc agctaccggt
     8461 aagccgctgt tctcttctca catgatcgac ctgtctgaag aatctctgca agagaacatc
     8521 gaaatctgct ctaaatacct ggagcgcatg tccaaaatcg gcatgactct ggaaatcgaa
     8581 ctgggttgca ccggtggtga agaagacggc gtggacaaca gccacatgga cgcttctgca
     8641 ctgtacaccc agccggaaga cgttgattac gcatacaccg aactgagcaa aatcagcccg
     8701 cgtttcacca tcgcagcgtc cttcggtaac gtacacggtg tttacaagcc gggtaacgtg
     8761 gttctgactc cgaccatcct gcgtgattct caggaatatg tttccaagaa acacaacctg
     8821 ccgcacaaca gcctgaactt cgtattccac ggtggttccg gttctactgc tcaggaaatc
     8881 aaagactccg taagctacgg cgtagtaaaa atgaacatcg ataccgatac ccaatgggca
     8941 acctgggaag gcgttctgaa ctactacaaa gcgaacgaag cttatctgca gggtcagctg
     9001 ggtaacccga aaggcgaaga tcagccgaac aagaaatact acgatccgcg cgtatggctg
     9061 cgtgccggtc agacttcgat gatcgctcgt ctggagaaag cattccagga actgaacgcg
     9121 atcgacgttc tgtaagatat tcctttctgc ttatctcaag gcccgctctg cgggtctttt
     9181 tttcgccaaa agcaaaattg cctgatgcgc tacgcttatc aggcctatac gtatcctgca
     9241 atatattgaa tttgcaggtt tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
     9301 acaaagcgca cattgatatc tgtctgagcc ccgctctgcg gggatttttt cgccaaaagc
     9361 aaggtgattc agatgagaaa tctgtgatct atttggcaaa attatgcttt attgtttacc
     9421 cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc tccattttga gtcagttgaa
     9481 aaggaatatt gaatggaaga tttgaatgtt gtcgatagca taaacggcgc gggaagctgg
     9541 ctggtagcta accaggcgct gctgctaagt tatgcagtaa acatcgtggc ggcactcgcg
     9601 atcatcatcg ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa tcgcctgatg
     9661 atctcccgta aaatcgatgc cactgttgct gattttcttt ctgcattagt ccgttacggt
     9721 attatcgcct ttacgctaat cgctgcactg ggacgcgtgg gtgtacaaac cgcgtcagtc
     9781 attgctgtac tcggtgccgc aggcttagct gttggtctgg ctttgcaggg gtcactgtct
     9841 aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg agaatatgtt
     9901 gacctgggcg gcgtagccgg tactgtgctg agtgtgcaga ttttctccac caccatgcgt
     9961 actgcagacg gtaaaattat cgttattccg aacggtaaaa ttattgccgg aaatattatt
    10021 aacttctccc gcgagccagt tcgccgtaac gaatttatta ttggcgtggc gtatgattcc
    10081 gatatcgatc aggttaagca gatcctgacc aatattatcc agtctgaaga tcgcattttg
    10141 aaagatcgcg aaatgactgt gcgcctgaac gaacttggtg catcgtcgat taatttcgtg
    10201 gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt actgggatgt gctggagcgt
    10261 attaaacgtg aatttgatgc cgccggtatc agcttcccgt acccgcaaat ggatgtgaac
    10321 tttaagcggg tgaaagaaga caaagctgcg taatcaacgc tgaggccaga taatactctc
    10381 tggcctctct cttattagtt tttctgattg ccaattaata ttatcaattt ccgctaataa
    10441 caatcccgcg atatagtctc tgcatcagat acttaattcg gaatatccaa cgtgttttct
    10501 tattactttc aaggtcttgc acttggggcg gctatgatcc taccgctcgg tccacaaaat
    10561 gcttttgtga tgaatcaggg catacgtcgt cagtaccaca ttatgattgc cttactttgt
    10621 gctatcagcg atttggtcct gatttgcgcc gggatttttg gtggcagcgc gttattgatg
    10681 cagtcgccgt ggttgctggc gctggtcacc tggggcggcg tagccttctt gctgtggtat
    10741 ggttttggcg cttttaaaac agcaatgagc agtaatattg agttagccag cgccgaagtc
    10801 atgaagcaag gcagatggaa aattatcgcc accatgttgg cagtgacctg gctgaatccg
    10861 catgtttacc tggatacttt tgttgtactg ggcagccttg gcgggcaact tgatgtggaa
    10921 ccaaaacgct ggtttgcact cgggacaatt agcgcctctt tcctgtggtt ctttggtctg
    10981 gctcttctcg cagcctggct ggcaccgcgt ctgcgcacgg caaaagcaca gcgcattatc
    11041 aatctggttg tgggatgtgt tatgtggttt attgccttgc agctggcgag agacggtatt
    11101 gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa acagaggcgc
    11161 taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga gagactaacg
    11221 tgaagttcaa agttatcgcc ctggcggcat taatgggtat tagcgggatg gcagcgcagg
    11281 ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagc gtggatgcgg
    11341 tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat gccgctactg
    11401 ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgaa ctcaatcaga
    11461 tcgcgaaaaa agatatcagc tcagcgaact tacgcaccca gccagattat gattatcagg
    11521 atggtaaaag tatccttaaa ggctaccgcg ctgtgagaac ggtggaagtc acgctccgtc
    11581 agttagacaa actgaattcc ttgctggatg gcgcgctgaa ggcgggtctt aacgaaattc
    11641 gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg cgtaaggcag
    11701 cgattgataa cgcgattcat caggcgcagg aactggcgaa cggctttcat cgtaaactgg
    11761 ggccggtata tagcgtgcgc taccatgttt ccaactatca gcccagccca atggtgcgga
    11821 tgatgaaagc cgatgccgcg ccggtgtccg cccaggaaac ttacgagcag gccgctattc
    11881 agtttgatga tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa caacccgcta
    11941 aaacacctgc agcacaataa tatttgtagc ccgacgtatt cgtcgggcta atgcctttac
    12001 ccgatattgc ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc acctatcttt
    12061 gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag gatgcggggg
    12121 agggggatgg acatttttat ttcgaaaaag atgcgcaatt tcattttatt agcgcaaacc
    12181 aataatattg cccgggcggc agaaaaaatc catatgacgg cttcgccatt tggtaaaagc
    12241 attgccgcgc tggaagaaca aattggctat acgctattta cccgcaaaga taataatatc
    12301 agcctcaata aagccggaca ggaactgtat caaaaactgt tcccggttta tcaacggctt
    12361 tctgcgattg ataatgaaat ccataactca gggcgtcgtt cacgagaaat tgtgattggc
    12421 atagataata cttatccgac aattattttc gatcagttga ttagtctcgg agataagtat
    12481 gaaggcgtga ccgcccagcc agttgagttc agcgaaaatg gcgttatcga taatcttttt
    12541 gaccgccagt tagattttat tatctcccca cagcatgtat ctgcacgagt tcaggaactg
    12601 gaaaacctga ctatcagtga actaccaccg ctgcgattag gctttctcgt ttctcgccgt
    12661 tatgaagagc gacaagagca agagctgctt caggagctgc cctggctaca aatgcgcttt
    12721 cagaaccggg ccaacttcga agcaatgatt gatgcgaata tgcgaccttg tgggatcaat
    12781 ccaaccatta tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt ggagcgcgga
    12841 cattttctga cggtgatccc gcattttgcc tggcgactgg tgaatccggc aacgctgaaa
    12901 tattttgatg caccgcacag gccgatgtat atgcaggaat atctttattc aatcagaaat
    12961 catcgttata ccgccacgat gcttcagcat attgctgaag atcgtgacgg gacgagtcat
    13021 taacccagca tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga
    13081 tcgtggtgaa tatgtccgcc aggcgcattt tccagatagc gatgcagata atcccgatac
    13141 ataggatgtg cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc
    13201 agatcggcga tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca
    13261 tggctgcaca ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggggccata
    13321 aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga
    13381 tcgaccccgg ctacgtgtgt tgagttggca tgcccgtaaa tatcaaactc caggccgacg
    13441 ttcagagcga tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga
    13501 cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg
    13561 gccgagattg tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc
    13621 accaccgatt cctgtagcac ttccgaatac atcataaacg gaggaattac cgggttttcc
    13681 cccagacgcg ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat
    13741 tccggcggaa tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca
    13801 atctgctggc acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt
    13861 tccacgacgg cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc
    13921 attgcatgga agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatcc
    13981 gccagttctg caacgcgcgg atcgtgatag tgattgagtt caatgatcac tttcttcgcc
    14041 cgcagcagcc aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt
    14101 gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc
    14161 atttgcgcca cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt
    14221 ttacgtaaac cggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa
    14281 agtacatcgt cagcggcggc gctgattgac gcacccgtca gaaggcgaat ttgatacggc
    14341 tttttggcct catgctgttc gttagctctg cgggcaatcg cggtgggtag ggctttcggc
    14401 gaacccgccg gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc
    14461 gcttcattgg cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg
    14521 attaatgacc aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt
    14581 aatcttcgct gtcatacacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt
    14641 tcatggtgtg tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg
    14701 gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt
    14761 ccacaacatg gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg
    14821 taaaaatcag ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga
    14881 ccaggttata cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg
    14941 cgatgatcag atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca
    15001 ccatcgaaat gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat
    15061 catcatagga gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg
    15121 agaagacttt ggatccactc ggtgcgcgca aaatgataca gcgaatttcc ggccggttga
    15181 gatcgcttaa cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc
    15241 ggccatagtt aaactcaatg accgccactt tgttgatagt gacaacgtta acatactgat
    15301 aagacataaa aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc
    15361 aggccggtgc gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag
    15421 cgatcgaaat cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc
    15481 agccattcca ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg
    15541 gttttgaagt cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta
    15601 caagtcagaa cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc
    15661 tcgtacatat gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata
    15721 acgatcagat cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca
    15781 ccggcaattt gcaacgagat aaaacagtcc accatgcggg cgacttctgt ttccgactgc
    15841 ccgacgccaa ccgtttcgac aatcactacg tcataacccg ctgcttcgca taacagcatt
    15901 aattcccgcg ctcgctgact ggcaccgccc agatgaccgg aggatggtac cgggcgaata
    15961 aacgccgctt cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg
    16021 ccagtgaccg ggctgctggg atcgaccgca ataaccgcga cctttaatcc ctctcgaatc
    16081 aacaacatgc caaagtcctc aagaaaggta cttttccccg cgccgggggt gccggtaacg
    16141 cccagtcgca gggtgttacc gcagtacggc ataatggcat caagcagctg cgtacttagt
    16201 gcctgatgac gcgggtgacg gctttccacc agcgtcatgg cctgggcgag tgtggcacgc
    16261 tcaccctgac gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg
    16321 gcttatcaga ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat
    16381 cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc
    16441 gaccacgcag atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag
    16501 cgttttatga ccggcagcca gtgaggatgc gcccactacg taaacgtcgt tttctacggc
    16561 caggcgggcg atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag
    16621 atcggaatag gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt
    16681 agcgatcaga atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac
    16741 aatggcatcg aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc
    16801 ggtaacacac tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat
    16861 ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt
    16921 atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt
    16981 ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc
    17041 aagtacatcg gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg
    17101 cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg
    17161 cagacctgct tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc
    17221 tctggcttgt ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg
    17281 atcgacggtg cggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat
    17341 gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga
    17401 ctgagtaccg cccagcgtcg cagccagcgc ttcaatggtg gtgcggataa cgttgttata
    17461 cggatcctgt tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga
    17521 tttcgggtcc tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg
    17581 caacatggcg acgttcataa acagatccat gccgatgccg aagaagaacg acaggcgagg
    17641 agcgaagtca tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc
    17701 agcgagcgta aatgctacct gctgcacgca gttggcaccc gcttcaccca tgtggtaacc
    17761 gctgatactg atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc
    17821 gataatgcgc attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt
    17881 gagaatatcg ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc
    17941 ggcgacgata taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga
    18001 cattttatcc agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc
    18061 gacgcccgct ttgccgacgt cgcccgccac gcgcgggtta tcggagtcgt agccacggtg
    18121 ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata
    18181 aaaagcgttg gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg
    18241 ggcggtatac atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc
    18301 tgtcacctcc agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc
    18361 cgcggtttga tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc
    18421 aagctgttgc cactcctgca cgttagacat tatttgctcc gtaaaacgca atcaaaatga
    18481 gggtatttgc gcctacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat
    18541 tcagacgtcg ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac
    18601 aaaaagaagt gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc
    18661 tgacgaagga ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg
    18721 ctttcaggag caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag
    18781 tcaatcagtt caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag
    18841 gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggc
    18901 acgctgcctg gaggcagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga
    18961 tggtcaaacg cgaccactgg cgctttcagt aatgccgaac gcgttacgcc gttagggaaa
    19021 tatttttcgg caaagggttt tgagctgacg aacagatagt cgagcgcacc aagtttatcg
    19081 acaagacaac tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg
    19141 cgcagacgtt cctgagtgcg ggtttcatct tctacctgca agttgaggcg gataggcgaa
    19201 tcagccaaca caggagccag tgcaggaagc aaccacgtcg ccagactgtc ggcgttgacc
    19261 gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc
    19321 agcaactcca cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc
    19381 ggtacggtac gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt
    19441 gagacggctg attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt
    19501 atcaccgcat ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc
    19561 tcctggtaaa tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga
    19621 agccgtcggc gggtaacatt tgtattcgtg gttaaacgcg cagcttaaaa gatcagggat
    19681 ttgcgctaat gcatttcgct cagcgagttc gcgccctggt tgtgttgaat ggcgtggcgt
    19741 tattgcctca atttgcctgt aaacaggggc ttgtgaacgg tgaactggtg cgcctgtttg
    19801 ctccgtggag cggcataccc agattgttgc atgccttatt tgcggggcga aaggggatgc
    19861 ctgccattgc gcgatatttt atggatgagt taaccacgcg gcttgccaac ggggtctgaa
    19921 tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg
    19981 caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggcacc
    20041 attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcgct cggtacaatg
    20101 aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc
    20161 ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc
    20221 gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt
    20281 gaaaaaatca ttgcttcggt tgcagaaaaa tttatctgta ttgcagacgc ttccaagcag
    20341 gttgatattc tgggtaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca
    20401 gtggcgcgcc agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtggtgacc
    20461 gataatggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg
    20521 gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc
    20581 gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac
    20641 gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt
    20701 tttacctggc tattgtcgat tgctctaaat aaatcttcta aaccagcata ttcatccaag
    20761 aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata
    20821 ttattttttg atatgttgaa aggcggacgc aaatccgcac acaacatttc aaaagacagg
    20881 attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
    20941 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
    21001 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
    21061 ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc
    21121 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
    21181 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt
    21241 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcaccgtggc
    21301 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
    21361 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
    21421 tacttttatg atattgaaaa taaactgccg ctgggcaacg ccactcaggt acagcatctt
    21481 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtccacc
    21541 aaaaatatga tgggcgcgaa agaaatttca ctaatgaagc ccggctcgct gctgattaat
    21601 gcttcgcgcg gtactgtggt ggatattccg gcgctgtgtg atgcgctggc gagcaaacat
    21661 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
    21721 acctctccgc tgtgtgaatt cgacaacgtc cttctgacgc cacacattgg cggttcgact
    21781 caggaagcgc aggagaatat cggcctggaa gttgcgggta aattgatcaa gtattctgac
    21841 aatggctcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcacggtggg
    21901 cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct gaacaaaatc
    21961 ttcgccgagc agggcgtcaa catcgccgcg caatatctgc aaacttccgc ccagatgggt
    22021 tatgtggtta ttgatattga agccgacgaa gacgttgccg aaaaagcgct gcaggcaatg
    22081 aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aattcccctt ctctgaaaat
    22141 caacgggcag gtcactgact tgcccgtttt tttatccctt ctccacaccg ttgccgcgtt
    22201 gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt
    22261 gtttatttaa tggggattta cgatggcagg gcagcatggg gctgtaacgg taaagccctc
    22321 accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
    22381 ctgttactca taagtttcag cgcttattaa cagtcagtct caggggagga gcaatcctcc
    22441 cttaccctta ctcactaaat taggtcaaag aatcaacgat gtcaatcagg gcgatgcggg
    22501 tgtatcgccc ttaccactcc cagactttcg acggtgtaac caccgcagga agagggatat
    22561 cccactcttc aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt
    22621 gcgttttata gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca
    22681 ggcgctgacc gtactcatca aaggcgacca gcggtgtgat cagcacgtct aatcgggaaa
    22741 ggggtagcac gtcacgcaca tccaattttg gctcatggat cttcaacctg ttcatcacca
    22801 gttcgctttg cggatggtaa ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa
    22861 ctggaagata tacgcgctta ccggcgcgcc agagttgttc tatgagtggc tgggtgtcga
    22921 gttcgccatc aaaagagagg aatacagcga ccgtatgtgc catcaccacc gggggataag
    22981 tcatcatccg ggtagcggct tgttgaccca tttcctgctg ttgttccggc gttaacgcac
    23041 gacgacgttg ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagttctg
    23101 gtagttgcgt catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct
    23161 gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga
    23221 cggaccgagc atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct
    23281 caggggactg gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc
    23341 acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg
    23401 ttcaagtaac gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc
    23461 cgcgtagtca cgagtctttg ctttttcttg cgctaactca tagctgatat tcaatgcggc
    23521 aatgaagacc aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg
    23581 ttggttcaga tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac
    23641 acgcagtgaa cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg
    23701 ctgattgact gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag
    23761 ctaccctgat gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat
    23821 atcatgaata ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg
    23881 gttacaacga aatgaaccag tatctgaacc aacaagggac gggtctgacc ccagctgaga
    23941 tgcatggttt aatcagcggg atgatatgtg gcggtaacga tgacagctca tggctaccgc
    24001 tacttcacga cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcactgc
    24061 gtaaaatgca ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt
    24121 atctgcctga tggcgatgat gtcagcgttt tcgatcgggc tgatgcattg gcaggttggg
    24181 tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa gctggataaa gtgaccggcg
    24241 aaaccggtga agctatcgac gatctgcgta acattgcgca actgggttac gacgaagacg
    24301 aagatcagga agagcttgaa atgtcgcttg aagagatcat cgaatacgtt cgtgttgccg
    24361 cgctgttatg ccacgacacc tttactcatc tgcaaccgac cgcgccagaa gtacaaaaac
    24421 cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta tgagtgatat atcccggcaa
    24481 gagtttcagc gtcgccgtca ggccctggtg gagcaaatgc aacccggcag cgccgcgctg
    24541 atttttgctg caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac
    24601 agtgacttct ggtacttcac cggctttaac gaaccggaag cggtgctggt gctgattaaa
    24661 agcgatgaca ctcataacca cagcgttttg tttaaccgcg ttcgcgacct gacggcggag
    24721 atctggtttg gccgtcgttt aggtcaggat gccgcgccag agaaactggg cgttgaccgc
    24781 gcactggcat tcagcgaaat taatcagcaa ctttatcaac tacttaatgg tctggatgtg
    24841 gtttaccacg cccagggcga atatgcatat gctgatgaaa tcgtgaacag cgcgcttgag
    24901 aaactgcgta aaggttcgcg gcaaaatctc accgcgccgg caacgatgat cgactggcgt
    24961 cccattgttc atgaaatgcg cctgttcaaa tcgccggaag agattgccgt acttcgccgc
    25021 gcgggtgaaa tcaccgccct ggcgcatacc cgggcgatgg aaaaatgccg tccgggaatg
    25081 ttcgagtacc acctggaagg cgaaattcac cacgaattta accgccacgg cgcgcgctat
    25141 ccttcctata acaccattgt cggcagcggt gaaaacggct gcattctgca ctacaccgaa
    25201 aacgagtgtg aaatgcgcga cggcgacctg gtgttgattg acgcgggttg tgaatacaaa
    25261 ggttacgctg gcgatattac ccgcaccttc ccggtcaacg gcaaattcac ccaggcccag
    25321 cgtgaaatct acgacattgt gctggagtct ctcgaaacca gcctgcgcct gtatcgtccg
    25381 ggaacttcca ttctggaagt cactggtgaa gtggtgcgca tcatggttag cggcctggta
    25441 aaactcggca tcctgaaagg tgatgttgat gaactgatcg ctcagaacgc ccatcgtcct
    25501 ttctttatgc atggccttag ccactggtta ggactggatg tccatgacgt gggtgtttat
    25561 ggtcaggatc gctcgcgcat tctggaaccg ggcatggtac tgaccgtaga gccagggctg
    25621 tatattgcgc cggatgcaga agtgccagaa caatatcgcg gtatcggcat tcgtattgaa
    25681 gacgacattg tgattaccga aaccggtaac gaaaacctca ccgccagcgt ggtgaaaaag
    25741 ccggaagaaa tcgaagcgtt gatggttgct gcgagaaagc aatgagcgta atcatcgtcg
    25801 gtggcggcat ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc
    25861 tgccggtaca tttgattgaa gcgactgcga cagagtcaca tgctcatccg ggctttgatg
    25921 gacgagcgat agcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc
    25981 aatctctggc ggattgcgca actgccatca ccaccgtgca tgtcagcgat cgtggtcacg
    26041 ctggatttgt caccctcgcc gcagaagatt accaactggc ggcgctggga caggttgtcg
    26101 aattgcacaa tgtcgggcaa cggctgtttg cattgctgcg taaagcacct ggcgtaacgc
    26161 tgcattgccc tgatcgcgtg gctaacgttg cccgtactca gagtcacgtt gaagtgacgc
    26221 tggagagtgg cgagacgctg acgggccgcg tgctggtagc agctgatggc acccattcag
    26281 cgttagccac cgcgtgcggc gttgactggc agcaggagcc ttacgaacaa ctggccgtga
    26341 ttgccaacgt tgctacttcc gttgcgcatg aagggcgcgc ttttgaacgc tttacgcaac
    26401 atggcccgct ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc
    26461 cactggaacg gcgcgaagag gtgttgtcgt ggagtgacga gaagttttgc cgtgaactcc
    26521 agtcggcctt tggctggcga cttgggaaaa ttacccacgc tggtaaacgc agtgcttatc
    26581 cgctggcgtt aacccacgcc gccagatcta ttacccatcg taccgtgctg gtgggcaatg
    26641 cggcgcaaac tctgcacccg attgccgggc aagggtttaa cctcggtatg cgagatgtga
    26701 tgagtcttgc ggaaaccctg actcaggcgc aggagcgcgg agaagacatg ggggattacg
    26761 gcgtattgta ccgttatcag cagcgtcgac agagcgatcg cgaagcaacc attggcgtca
    26821 cggacagcct tgtacatctt tttgccaacc gttgggcacc gctggttgtc gggcgcaaca
    26881 tcgggctgat gacgatggaa ttattcaccc cggcacgcga tgtgctggcg cagcgcaccc
    26941 tcggttgggt ggcgcgttga aggcggtttt aaggaataaa caatgcaaag tgttgatgta
    27001 gccattgttg gcggcggcat ggtggggctg gcggttgcct gtggcttaca ggggagcggc
    27061 ttacgcgttg ccgtactgga gcagcgcgta caggaacctc tggcggcgaa tgcaccacca
    27121 caactgcgcg tttcggctat caatgccgcc agcgaaaaat tactcacccg tcttggcgtc
    27181 tggcaggaca ttctctctcg tagggccagc tgttatcacg gtatggaagt gtgggacaaa
    27241 gacagctttg gtcacatttc gtttgacgat caaagcatgg gctatagcca tcttgggcat
    27301 atcgttgaaa attcagtgat tcactacgcg ctgtggaaca aagcgcatca gtcgtcagat
    27361 atcactctgt tagcccccgc agaattacag caggtcgcct ggggagaaaa tgaaaccttc
    27421 ctgacgctga aagatggcag catgttaacg gcgcgtctgg tgattggcgc ggacggcgct
    27481 aattcctggt tgcgcaacaa agccgatatt ccgctgactt tctgggatta tcagcatcac
    27541 gcgctggtag cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg gcaggttttc
    27601 catggcgaag gcattctggc ctttttaccg cttagcgatc cgcatctttg ctcgattgtc
    27661 tggtcactgt cgccagagga agcgcagcgg atgcagcagg caagtgaaga cgaatttaat
    27721 cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat gcaaggttga gagcgcgcgt
    27781 caggtgttcc cactgacggg gcgttatgcg cgccagtttg cctcgcaccg tctggcgctg
    27841 gtgggcgacg ccgcacatac cattcacccg ctggcggggc agggggtaaa tctcggcttt
    27901 atggatgctg cagagctgat tgccgaactg aaacggttgc atcgtcaggg gaaagacatc
    27961 gggcagtaca tttatctgcg tcgctatgag cgtagccgca agcacagtgc ggcgttgatg
    28021 ctggctggta tgcagggatt ccgcgatctg ttttccggta ccaatccggc gaaaaaactg
    28081 ctgcgtgata ttggtttgaa actggccgac acgcttcctg gcgttaagcc gcaacttatc
    28141 cgccaggcaa tgggattaaa cgatttgcct gaatggctgc gttaaaaatt tctcctctgt
    28201 tgtttatttg atacccatca cactttcatc tcccggtttt ttcgccggga gattttcctc
    28261 atttgaaata aactaatttc acctccgttt tcgcattata ttttctaatg ccattatttt
    28321 ttgatttagt gttttttgac atttttttag ctcttaatat tgtcttattc aaattgactt
    28381 tctcatcaca tcatctttgt atagaaactg gtgtattttt tggtttttta ttctgtcgcg
    28441 atttttgcat tttttaacca taagctaatg tgatgatcaa ttttacctta tggttaacag
    28501 tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc gttggggacc gggagtggct
    28561 ccgatgctgg gtttcgtggt gataatttca ccatgaaaaa gttgtcagcc ccgcttattc
    28621 aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct ttgcggcgct
    28681 cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca aatcgacgaa
    28741 catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac catcgtcgat
    28801 cttcgcggca gccgcacccg ggagtttctg cgttatctgc tggcgaacga tgtggcgaag
    28861 ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg cggtgtgata
    28921 gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt taactccgcc
    28981 acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg catcgaaatt
    29041 accgttcgtg atgacctttc catgattgcc gtgcaagggc cgaatgcgca ggcaaaagct
    29101 gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc gttctttggc
    29161 gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc gggctatgaa
    29221 attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga agcgggtgtt
    29281 aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat gaatctttat
    29341 ggtcaggaga tggacgaaac catctctcct ttagccgcca acatgggctg gaccatcgcc
    29401 tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc tggaagtgca gcgtgagcat
    29461 ggtacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg taatgaactg
    29521 ccggtacgct ttaccgatgc gcagggcaac cagcatgaag gcattatcac cagcggtact
    29581 ttctccccga cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg tattggcgaa
    29641 acggcgattg tgcaaattcg caaccgtgaa atgccggtta aagtgacaaa acctgttttt
    29701 gtgcgtaacg gcaaagccgt cgcgtgattt acttttttgg agattgattg atgagcaacg
    29761 taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa gccgacggca
    29821 cttacaccgt tggtattacc gaacatgctc aggagctgtt aggcgatatg gtgtttgttg
    29881 acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt gccgaatcgg
    29941 taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg gtaaacgacg
    30001 cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggcggc tggatcttta
    30061 aaatcaaagc cagcggtgaa agcgaactgg aatcactgct ggatgcgacc gcatacgaag
    30121 cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat cagggtgagg
    30181 aaaatttatg cctcaccctc actctcttcg taaggagaga ggttcacaat tcactgcacg
    30241 tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag cggcgctttt
    30301 attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa tgccgttggt
    30361 gcacaatcgt taaacgcgct gaccggccag attgtgccga aagatattca acttgcgaca
    30421 ccaccgcagg ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact caaggctatt
    30481 gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc cgtgcagcta
    30541 ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc gtacactccg
    30601 tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca gcaggtaacg
    30661 ctggatttga ctggactgga tatggcctct gcttctcttc tggacgaggc caccgctgcc
    30721 gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttc
    30781 gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc cgaaaccttt
    30841 ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga cgtcttcggc
    30901 gtgctgttac agcaggtagg cactaccggt gaaattcacg actacactgc gcttattagc
    30961 gaactgaaat cacgcaaaat tgtggtcagc gttgccgccg atattatggc gctggtgctg
    31021 ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca acgcttcggc
    31081 gtgccgatgg gctacggtgg cccacacgcg gcattctttg cggcgaaaga tgaatacaaa
    31141 cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa taccgcgctg
    31201 cgcatggcga tgcagactcg cgagcaacat atccgccgtg agaaagcgaa ctccaacatt
    31261 tgtacttccc aggtactgct ggcaaacatc gccagcctgt atgccgttta tcacggcccg
    31321 gttggcctga aacgtatcgc taaccgcatt caccgtctga ccgatatcct ggcggcgggc
    31381 ctgcaacaaa aaggtctgaa actgcgccat gcgcactatt tcgacacctt gtgtgtggaa
    31441 gtggccgaca aagcgggcgt actgacgcgt gccgaagcgg ctgaaatcaa cctgcgtagc
    31501 gatattctga acgcggttgg gatcaccctt gatgaaacaa ccacgcgtga aaacgtaatg
    31561 cagcttttca acgtgctgct gggcgataac cacggcctgg acatcgacac gctggacaaa
    31621 gacgtggctc acgacagccg ctctatccag cctgcgatgc tgcgcgacga cgaaatcctc
    31681 acccatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta tatgcactcg
    31741 ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg
    31801 aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc cgaactgcac
    31861 ccgttctgcc cgccggagca ggctgaaggt tatcagcaga tgattgcgca gctggctgac
    31921 tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc cggcgcacag
    31981 ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa tgaagggcat
    32041 cgcgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc tgcacatatg
    32101 gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga tctgactgat
    32161 ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt gacttatcct
    32221 tctacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc
    32281 gacggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat cacctcgccg
    32341 ggctttattg gcgcggacgt ttcgcacctc aacctgcata aaactttctg cattccgcac
    32401 ggcggtggtg gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc accgtttgta
    32461 ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc agtttctgcg
    32521 gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg catgatgggc
    32581 gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta tattgccagc
    32641 cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt ggcgcacgaa
    32701 tgtattctcg atattcgccc gctgaaagaa gaaaccggca tcagcgagct ggatattgcc
    32761 aagcgcctga tcgactacgg tttccacgcg ccaacgatgt cgttcccggt ggcgggtacg
    32821 ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt tatcgacgcg
    32881 atgctggcta tccgcgcaga aatcgaccag gttaaagccg gtgtctggcc gctggaagat
    32941 aacccgctgg tgaacgcgcc gcacattcag aacgaactgg tcgccgagtg ggcgcatccg
    33001 tacagccgtg aagttgcagt attcccggca ggtgtggcag acaaatactg gccgacggtg
    33061 aaacgtctgg atgatgttta cggtgaccgt aacctgttct gctcctgcgt accgattagc
    33121 gaataccagt aattcactga ttcgactatc ttctaaaggc gcttcggcgc ctttttagtc
    33181 agatgacaaa gtacaaaagc gctcagacag tcccctcacc cctttgggga gagggttagg
    33241 gtgaggggaa caggccggca ctggcgcgaa tatttaccct caccccggcc ctctccctga
    33301 aagggcgagg ggaaaagcgt gccaacattg aagattgagc cagtttgtta gcaatctcaa
    33361 agatacgtca acgaattaat ttttctcgga aaaacaaatg gctatagcac ttgtgaccgg
    33421 tggcagtcgc ggcatcgggc gggcaactgc attactgttg gcgaaagaag agtatacggt
    33481 ggcggttaat tatcaacaaa acctccatgc ggcgcaggaa gtggtgaact taatcacgca
    33541 agccggtggc aaggcattcg tgctccaggc ggatatcagc gacgaaaatc aggtcatcgc
    33601 aatgtttaca gcaatcgatc agcacgatga accgctagca gcgctggtca ataacgccgg
    33661 gatcttgttt acccagtgca ccgttgaaaa ccttaccgca gagcgaatca accgagtact
    33721 ttccaccaac gtgacgggat attttctctg ctgccgcgag gcggtaaaac gcatggcgct
    33781 taaaaatggt ggcagtggcg gcgctatcgt caatgtctct tcggtggcct cacggttggg
    33841 ttcgccaggg gaatatgttg attacgcggc atcgaaaggg gcgattgata cgttaaccac
    33901 cggactatcg ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc ggccagggtt
    33961 tatttatacc gaaatgcacg ccagcggcgg cgagcctgga cgcgtcgatc gcgttaagtc
    34021 gaacatcccc atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca ttgtctggct
    34081 actaagtgat aaagcctctt acgtcacggg aagttttatc gatttggcgg gcgggaaata
    34141 aaacagggaa gttgtctgac cggatgcaac aagtattgca tccggtactt catcgactta
    34201 aagcttctcg ccgttgctgg caatcacctc tttgtaccag ttaaagctct tcttacgtga
    34261 acgcgacata tcgccagtac cgtcgtcatg tttattcaca tagataaagc cgtagcgttt
    34321 gctgtactgc ccggtagtga atgacacgca gtcgatgcag ccccacggtg tgtagcccat
    34381 cagatccaca ccatcgtaag tcactgcttt tttcatctct tcaatatggg cgcgcaggta
    34441 gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa
    34501 accgttttcg acaataaaca gcggcctctg ataacgctca tacagttcgc aaagggcata
    34561 gcgcagacct actggatcaa tctgccagcc ccagtcagac gctttaacat acgggttcgg
    34621 tacgctgcct tcaaaaccag agatcgcatc gccggtgccg ccttcggcct tcactgcgtt
    34681 ggtcatgtaa tagctgaaac caagataatc gcaggtgcct tcacgcagta catccagatc
    34741 gccatcttcc attttgatgt taaatccgcg acgctcccac tcgttcaaca cataggacgg
    34801 gtaatagccg cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc
    34861 gaacatcaca tcgtccgggt tacaggagta gggatagagc ggcaccatcg ccagcataca
    34921 gccgactttc atctccgggt taatgcgacg cgcggctttc actgccaggg cgctggcgac
    34981 aaactggtga tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct cggtatacac
    35041 cacgccggag cagcagtaac caaacagcgg cgcacgccag ttacgctggt tgtttatttc
    35101 gttgaaggtc atccagtatt tgactttatg cttgtagcgt tcgaatacca cttccgcgaa
    35161 acggacaaag aaatcaacga ctttacggtt ggtccagcta ccgtattgct gcaccagatg
    35221 cagcggcatt tcaaagtggg agagggttat caccggttcg atgttgtatt tcagcagttc
    35281 atcgaacata tcatcgtaga acttcagccc ttcttcgttt ggctgagctt catcaccttt
    35341 tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca tttcggcaaa
    35401 tagcttgatg tcttccttgt agtgaccata aaaatcaacg gcttcatggt ttggatagta
    35461 tttgcctggc aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag
    35521 aacgtcacaa atgctcggcc ctttgccgcc tttgttccag ccgccttcga cctgatgagc
    35581 ggcaactgcg ccgccccata agaaatcttt cggtaaggtg agttttttca ctatcatagg
    35641 ctccagatga gtcaattctt acaggaaata gtaccatgca gccaaacgac atcacttttt
    35701 ttcaacgttt ccaggatgac attctggctg ggcgtaaaac catcaccatc cgcgacgagt
    35761 ctgaatcgca cttcaaaacg ggtgatgtgc ttcgtgtcgg gcgttttgaa gatgacggtt
    35821 atttttgcac gattgaagtc accgcaacct caaccgtaac gctggatacg ctgacggaaa
    35881 aacatgcaga gcaggaaaat atgaccctga ctgaactgat aaaggtcatt gccgacatct
    35941 atcccggtca gacacagttt tatgtgattg aatttaaatg tctttaaatt gaactcacgc
    36001 ctgttagttg aaatttcgca tttaatttca tgatttttag gagattagtc tcaaatcaga
    36061 atttatttta gctaacaggt gttcactgga actattctca gttacgctgg agaggtatac
    36121 atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga agcccctcat taagcaggga
    36181 tattcgctgg cagaggaaat tgccaacagc gtcagtcacg gcattgggtt ggtgtttggt
    36241 atcgttgggc tggtgttgct actggttcag gcggtggatc tcaatgccag cgccacagcg
    36301 ataaccagtt acagcctcta tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc
    36361 tatcacgcca ttcctcatca acgggcaaaa atgtggctga agaaatttga ccattgcgct
    36421 atttacctgt tgattgccgg aacctacacg ccgtttttgc tggtggggct ggattctccg
    36481 ttagcgcgcg ggttgatgat tgttatctgg agcctggcat tgctgggtat tctgtttaaa
    36541 ctgaccatcg cgcaccgatt caaaatttta tctctggtga cctatctggc gatgggctgg
    36601 ctgtcgctgg tggtaattta tgaaatggca gttaagctcg cggcgggcag cgttacctta
    36661 ctggcggtag gcggcgtggt ttattcgctc ggggtgattt tctacgtctg caaacgcatt
    36721 ccatacaacc atgccatctg gcacggcttc gtgctcggcg gtagtgtgtg ccactttctg
    36781 gcgatctatt tgtatattgg gcaggcgtaa tgggtatgcc tttgtaggcc tgataagacg
    36841 cgccagcgtc gcatcaggca tcggagcaca actgccggat tcggcgtgaa cgccttatcc
    36901 gatctacggg cctcgtccct gttgtgataa aggcatcaaa tcccagacat gataagacgc
    36961 gccagcgtcg catcaggcga taaatgcctg atgcgataaa gagatttact cttcgagcga
    37021 atacggcagc ggctcgatat gcaatgtatt cgcatcgtcg cgtacgcgga agatgctatc
    37081 cggttccata tcgttattca tgacgacctg taccacgacc tgaccatctt ccagttttac
    37141 cgcagccagc accgtaccgg tacgacgcca gttctcgccc attttcagct ctaagtcttc
    37201 accagcttcc ggcagtcggc tggcgctacc tgccagcaac cagagcgcac gtttattggc
    37261 accacggaat tttgctcgcg ccaccatctc ttgtccggta taacagcctt tcttaaagct
    37321 gataccgccc agcgcctgga gattggtcgc ctgtgggata aactgcccgc tgttggcggc
    37381 atcaatcacc gggaaacccg cttcaatgtt taatgccagc cactgttggc tattgttcag
    37441 ttccgcttca ccgcgcagtt tatcggtcag catattagca gtagcttcat cggttacgat
    37501 caggaaacgt tctgccgggt gttcaaacca tagcaaagtg gtcgcgcctt ctttgactac
    37561 ctgtttttct ttcgaaggca gttcgctaaa gagatttgcc agcgcggcgc gcgcctgaaa
    37621 accggcaaca ccaagcagca cacgctcgtc gtctggcgcg atggtcactt tagagaatac
    37681 cgcatatttt ttcagttcag tcagctgcgg ttcacgcacg ctgcgccgtt caatccatgc
    37741 aaagccatcg ccgtcgcgga acagacgtaa attgctccac attttacctt tggcgtcgca
    37801 atgggcggcg agcaggtgct gatcttctgc catctggctg acatctgctg tcacctgacc
    37861 ctgcatatat ttttcgctgt ccgcgccagt aatggtggca agcgcccagt catcaagcgt
    37921 catcagcgtc agcggtaaac gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa
    37981 agccataata gagtcctgag ttgcttaacg gcgtattaat gactaatggt aaaagagcta
    38041 tgaggcaatg caagtgcttt cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa
    38101 aaatgaagcc attgaattgt tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat
    38161 tcccgtaaaa gaaggcgaaa aacacgttac aatagccggg tactggattt tcgtgagaca
    38221 ggaagaagat ggacattaac aacaaagccc gcattcattg ggcatgccgc cgtggtatgc
    38281 gcgaactcga tatttcaatc atgccgtttt tcgaacatga gtacgacagc ttaagcgatg
    38341 acgaaaaacg catctttatt cgtctgctgg aatgtgacga tccggacctg tttaactggc
    38401 tgatgaatca cggtaaaccg gccgatgcag aactggaaat gatggtccga ctcatccaga
    38461 cacggaaccg ggaacgtggt cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag
    38521 tggctttcct tgctgattca tgggctggtt gccgctgtta ttttactcat gccctggcca
    38581 ctcagttaca ccccgttatg gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc
    38641 agccagcggc gtattaatgc tcgccagggg gaaattcgct tgttgatgga cgggcgtttg
    38701 cgttggcaag ggcaggagtg gagcatcgtc aaagcaccgt ggatgattaa gagcggcatg
    38761 atgctgcgtt tacgttctga tggcggtaaa cggcaacatt tatggctggc agccgacagc
    38821 atggacgaag ctgaatggcg ggatttacgg cggattttgt tgcaacaaga gacgcaaaga
    38881 taaagatgac ggcaaagcat atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc
    38941 catttcgttg aggatttgct cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg
    39001 gttagtttca tccagcgcca gacccacgaa cagttgcccg tcagcaatca ccggtttcgg
    39061 gctggtaaat tcatatcctt ccgttggcca gtagccgacg aactttacgc ctttggtcga
    39121 gagtttgtca tgcagcatac cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc
    39181 gccaagccca tacagcgcaa caattttacc ttcaaggttc aggtcgtcga gctgatccca
    39241 gacggcttcc cagtcttcct ggatttcacc aaaatcccag gtcgggatac ccagaatgag
    39301 cacatcgtac tgctccatta atttcgggga gtcgtccttg aggttatgta aggtcaccag
    39361 ttctgggccg ataatatcgc ggattttttc tgccgccatt tcggtgtaac aggtgctgga
    39421 accgtaaaaa agacccatat tcataacgta agcttctcga ttctgctgtg gcttttgcag
    39481 ctagtgtatc agaatcgctt tagtcacggc acaataaggc ataatggatc aaatgagtga
    39541 ccataagggg cgcaagtgaa acaggatctg gcacgcatcg agcagtttct tgatgctctg
    39601 tggctggaaa aaaatctggc tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg
    39661 atggtggagt ggttgcatca ccgcgggttg acgctggcga cggcgcaaag tgacgatttg
    39721 caggcattac tggcagaacg gctggagggc gggtataaag ccaccagctc agcgcgtttg
    39781 ctgagtgcag tgcgccgatt gttccagtat ctttatcgcg aaaagtttcg tgaagacgat
    39841 cccagtgcgc atctcgcttc accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa
    39901 gcgcaggttg aacgtttatt acaggcacca ttaattgatc agccactgga gctacgcgat
    39961 aaagccatgc ttgaagtgtt gtatgctacc gggctgcgtg tctctgaact ggtcgggctg
    40021 acaatgagtg atatcagcct gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa
    40081 gagcgcctgg tgccgttagg tgaagaggcg gtttactggc tggaaaccta tctggagcat
    40141 gggcgtccgt ggctactgaa tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg
    40201 cagcagatga cgcgacagac cttctggcac cgcattaaac attatgccgt gctggcgggt
    40261 atcgacagcg aaaagctgtc accgcatgtg ttgcgtcacg cttttgccac tcacttatta
    40321 aatcatggtg cggatttacg cgtggtgcag atgctactgg gccacagcga tctctccacc
    40381 acgcaaattt atacgcatgt cgctaccgag cgtctgcggc aacttcatca acagcatcac
    40441 ccgcgggcgt gatgtctgaa aagaacggga agatttatga agaaaggttt tatgttgttt
    40501 actttgttag cggcgttttc aggctttgct caggctgatg acgcggcaat tcaacaaacg
    40561 ttagccaaaa tgggcatcaa aagcagcgat attcagcccg cgcccgtagc tggcatgaag
    40621 acagttctga ctaacagcgg tgtgttgtac atcaccgatg atggtaaaca tatcattcag
    40681 gggccaatgt atgacgttag cggcacggct ccggtcaatg tcaccaataa gatgttgcta
    40741 aagcagttga atgcgcttga aaaagagatg atcgtttata aagcgccgca ggaaaaacac
    40801 gtcatcaccg tgtttactga tattacctgt ggttactgcc acaaactgca tgagcaaatg
    40861 gcagactaca acgcactggg gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg
    40921 gacagcgatg cagagaaaga aatgaaagct atctggtgtg cgaaagataa aaacaaagcg
    40981 tttgatgatg tgatggcagg taaaagcgtc gcaccagcca gctgcgacgt ggatattgcc
    41041 gaccattacg cacttggcgt ccagcttggc gttagcggta ctccggcagt tgtgctgagc
    41101 aatggcacac ttgttccggg ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa
    41161 caccaaaaaa tgaccagcgg taaataattc gcgtgaaaca acagatacaa cttcgtcgcc
    41221 gtgaagtcga tgaaacggca gacttgcccg cggaattgcc tcccttgctg cgccgtttat
    41281 acgccagccg gggagtacgc agtgcgcaag aactggaacg cagtgttaaa ggtatgctgc
    41341 cctggcagca actgagcggc gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg
    41401 aaggaacgcg gattattgtg gtcggtgatt tcgacgccga cggtgccacc agcacggctc
    41461 taagcgtgct ggcgatgcgc tcgcttggtt gcagcaatat cgactacctg gtaccaaacc
    41521 gtttcgaaga cggttacggc ttaagcccgg aagtggtcga tcaggcccat gcccgtggcg
    41581 cgcagttaat tgtcacggtg gataacggta tttcctccca tgcgggcgtt gaacacgctc
    41641 gctcgttggg cattccggtt attgttaccg atcaccattt gccgggcgaa acattacccg
    41701 cagcggaagc gatcattaac cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg
    41761 caggcgtggg tgtggcgttt tatctgatgc tggcgctgcg cacctttttg cgcgatcagg
    41821 gctggtttga tgagcgtggc atcgcaattc ctaacctggc agaactgctg gatctggtcg
    41881 cgctgggaac agtggcggac gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc
    41941 aggggatgag tcgcatccgt gccggaaagt gccgtccagg gattaaagcg ctgctggaag
    42001 tggcaaaccg tgatgcacaa aaactcgccg ccagcgattt aggttttgcg ctggggccac
    42061 gtctcaatgc tgccggacga ctggacgata tgtccgtcgg tgtggcgctc ttgctgtgcg
    42121 acaacatcgg cgaagcgcgc gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa
    42181 aagagatcga acaaggaatg caaattgaag ccctgaccct gtgcgagaaa ctggagcgca
    42241 gccgtgacac gctacccggc gggctggcaa tgtatcaccc cgaatggcat cagggcgttg
    42301 tcggtattct ggcttcgcgc atcaaagagc gttttcaccg tccggttatc gcctttgcgc
    42361 cagcaggtga tggtacgctg aaaggttcag gtcgctccat tcaggggctg catatgcgtg
    42421 atgcactgga gcgattagac acactctacc ctggcatgat actgaagttt ggcggtcatg
    42481 cgatggcggc gggtttgtcg ctggaagagg ataaattcga actctttcaa caacggtttg
    42541 gcgagctggt taccgagtgg ctggaccctt cgctattgca aggcgaagtg gtgtcagacg
    42601 gcccgttaag cccggccgaa atgaccatgg aagtggcgca gctgctgcgc gatgctggcc
    42661 cgtgggggca gatgttcccg gagccgctgt ttgatggtca tttccgtctg ctgcaacagc
    42721 ggctggtggg cgaacgtcat ttgaaagtca tggtcgaacc ggtcggcggc ggtccgctgc
    42781 tggatggtat tgcttttaat gtcgataccg ccctctggcc ggataacggc gtgcgcgaag
    42841 tgcaactggc ttacaagctc gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta
    42901 tcatcgacaa tatctggcca atttagcgtc atcttctcta taaaaaagag cgtggattgg
    42961 gtacaatccc gctcttatca ccgcattttg actagctcaa taaaagaaat cagaccatgt
    43021 ttgaaattaa tccggtaaat aatcgcattc aggacctcac ggaacgctcc gacgttctta
    43081 gggggtatct ttgactacga cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg
    43141 gaacagccgg atgtctggaa cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc
    43201 tccctcgaag ccgttgtcga caccctcgac caaatgaaac aggggctgga agatgtttct
    43261 ggtctgctgg aactggctgt agaagctgac gacgaagaaa cctttaacga agccgttgct
    43321 gaactcgacg ccctggaaga aaaactggcg cagcttgagt tccgccgtat gttctctggc
    43381 gaatatgaca gcgccgactg ctacctcgat attcaggcgg ggtctggcgg tacggaagca
    43441 caggactggg cgagcatgct tgagcgtatg tatctgcgct gggcagaatc gcgtggtttc
    43501 aaaactgaaa tcatcgaaga gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc
    43561 aaaatctccg gcgattacgc ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg
    43621 gtgcgtaaga gcccgtttga ctccggcggt cgtcgccaca cgtcgttcag ctccgcgttt
    43681 gtttatccgg aagttgatga tgatattgat atcgaaatca acccggcgga tctgcgcatt
    43741 gacgtttatc gcgcgtccgg cgcgggcggt cagcacgtta accgtaccga atctgcggtg
    43801 cgtattaccc acatcccgac cgggatcgtg acccagtgcc agaacgaccg ttcccagcac
    43861 aagaacaaag accaggccat gaagcagatg aaagcgaagc tttatgaact ggagatgcag
    43921 aagaaaaatg ccgagaaaca ggcgatggaa gataacaaat ccgacatcgg ctggggcagc
    43981 cagattcgtt cttatgtcct tgatgactcc cgcattaaag atctgcgcac cggggtagaa
    44041 acccgcaaca cgcaggccgt gctggacggc agcctggatc aatttatcga agcaagtttg
    44101 aaagcagggt tatgaggaac caacatgtct gaacaacacg cacagggcgc tgacgcggta
    44161 gtcgatctta acaatgaact gaaaacgcgt cgtgagaagc tggcgaacct gcgtgagcag
    44221 ggaattgcct tcccgaacga tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa
    44281 ttcgacggta aagagaacga agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc
    44341 cgcatgatga cccgccgtat tatgggtaaa gcctcttttg ttaccctgca ggacgttggc
    44401 ggtcgtattc agctgtacgt tgcccgtgac gatcttccgg aaggcgttta caacgagcag
    44461 ttcaaaaaat gggatctcgg cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa
    44521 accggcgaac tgtctatcca ctgcaccgag ctgcgtctgc tgaccaaagc actgcgtccg
    44581 ctgccggaca aattccacgg cttgcaggat caggaagcgc gctatcgtca gcgttatctg
    44641 gatctcatct ctaacgatga atcccgcaac acctttaaag tgcgttcgca gatcctctct
    44701 ggcattcgcc agttcatggt aaatcgcggc tttatggaag ttgaaacgcc gatgatgcag
    44761 gtgatcccag gcggtgcggc tgcgcgtccg tttatcaccc atcataacgc gctggatctt
    44821 gacatgtacc tgcgtatcgc gccggaactg tacctcaagc gtctggtggt cggtggcttc
    44881 gagcgtgtat tcgaaatcaa ccgtaacttc cgtaacgaag gtatttccgt acgtcataac
    44941 ccagagttca ccatgatgga actctatatg gcttacgcag attacaaaga tctgatcgag
    45001 ctgaccgaat cgctgttccg tactctggca caggatattc tcggtaagac ggaagtgacc
    45061 tacggcgacg tgacgctgga cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg
    45121 atcaagaaat atcgcccgga aaccgacatg gcggatctgg acaacttcga ctctgcaaaa
    45181 gcgattgctg aatctatcgg catccacgtt gagaagagct ggggtctggg ccgtatcgtt
    45241 accgagatct tcgaagaagt ggcagaagca catctgattc agccgacctt cattactgaa
    45301 tatccggcag aagtttctcc gctggcgcgt cgtaacgacg ttaacccgga aatcacagac
    45361 cgctttgagt tcttcattgg tggtcgtgaa atcggtaacg gctttagcga gctgaatgac
    45421 gcggaagatc aggcgcagcg cttcctggat caggttgccg cgaaagacgc aggtgacgac
    45481 gaagcgatgt tctacgacga agattacgtc accgcactgg aacacggctt accgccgaca
    45541 gcaggtctgg gaattggtat cgaccgtatg gtaatgctgt tcaccaacag ccataccatc
    45601 cgcgacgtta ttctgttccc ggcgatgcgt ccagtaaaat aagcattacg ttatgctcat
    45661 aaccccggca aatgtcgggg ttttttaatt gtgctgcgcg aaagcagaca acaattttct
    45721 tgcttcacta ttggctgcct gcattaccat ccacggactg aacgcccacg gcgtggcatc
    45781 aataccgtgt aatacatctg ctaaatcaca ccattgataa tccatcactt catcatcgtt
    45841 gatctgtaac gcactgttgg tgcgtgcggc aaataccgga cacacttcat tttccacaat
    45901 gccattcgga tcggtggcgc gatagcgaaa gtcaggatag atagattcag gaggcgtaat
    45961 ttccacgcca agctcataac ggcaacggcg gatcactgcg tcttcgttgc tttctcccag
    46021 ttgtgggtgc ccacaaaccg agttagtcca cacgccaggc catgcttttt tgctcagtgc
    46081 gcggcgggta actaataatt gccccttggc attaaacagc caactggaga acgcgagatg
    46141 taagagggtg tctgccgtgt gtgcggcata cttttccagc gtacccgtgg gaactccctg
    46201 tgcattcaat aaaatgacgt gttccgtttg cataatttct cacatgtaat tctgatcact
    46261 catcattata gataattgaa gagtgaaatg attaaccaag tgagcgttta tcgacaaccg
    46321 cccgttttga gcggatgccg acaggtaaaa accatttaaa aaagtagcat catatatttg
    46381 aagcgacttt caatggccat tgcaacacag tcagaaatga caggaataga gagagtaaaa
    46441 agcaacagca gactgaatat agcgcgcatt aaaagattca tattattctc catgctcatt
    46501 tttcttcagc ttgcgcatca gaagccacat acgtactgtc ctgactttta aatctttatc
    46561 agagacggat tccttcacca ggtcagcatg atgctgatag ccattaaaga agacgttcag
    46621 cacaacggcg ctaagggttg cgagcataat gccgctatgt agcagcggtt gcagtacggc
    46681 cggtaactta gaaaagaaat cgtgagagag cgtcggagtc atgccaacgc cgagactgat
    46741 cgccacaata tagaggttgt aacggttggt ggtgtagtta cagcgcgaca gaattcgaat
    46801 ccctgtcgcc agtaccatgc cgaacatcac cagcccagcg ccgcccagca caaattgcgg
    46861 aatggaggcg accagcaccg ccatttttgg caccatgcca aacaggatta atataattcc
    46921 cgaagaaata cacacccagc ggctatgaac acgcgtcacg ctaaccaggc caacgttttg
    46981 tgaaaatgac gtgtggggga agctgttaaa cgttccgcct atcattgtcc ctacgccatc
    47041 gacacgcagc ccgcgaataa tatcgtgtga agagagttta cgaccgacta tttcacccag
    47101 tgccaggaac atccccatcg actcgataaa cacgatgatt aacacggcag tcatggtcag
    47161 aatggaaacg ggatcgaaaa tcggcatacc aaacgacatc ggggtgacaa tcgcaaacca
    47221 cgaggcatca tgtagcccgg ataagttgac ttcattcatc atccacgaaa gtaaaaagcc
    47281 aaatacaatc cccagtaata cggcgacgtt ggacataaat cctttcgcat agcgagtaat
    47341 gagcaagata aaaattaaga cggcaaagga gatacctaaa taaacgggat taccatattg
    47401 cggattccct ttacctccgg cggcccagtc aatacccacc tgaatgatgc taagcccaat
    47461 agaagtaata accacaccgg taaccagtgg cgggaataaa ggcatcaagc gaccgataag
    47521 tggcgctaat aatgtggtga taaaacccgc ggcgatagtg gcaccaaaga tccccagcag
    47581 gccgatatcc gggttcatcc caatggctat cattggtgtt acagcagcaa aggtcaccga
    47641 cataatcacc ggcaggcgga tccccataaa gcggccgata ccgatacatt gcaataatgt
    47701 gacgatcccg cagcaaaaga gatccgagct aatgagcatc gcaatagctt cttttgagag
    47761 gcccagtcgg tcaccaatca taagaggaac agcgactgca cctgcgtaca tgacaaggac
    47821 gtgttgcaga ccgagtatga tcagctttcc tggtgataat atgcggtcaa cctcatcagt
    47881 tgggcggtct tgcccagaag atgagggaag ttgggaatct atggcgctca tcagtgtttc
    47941 tcctttcgcc cgccagagta aaaagcaacc gtttacgtta atgaaactgt ctaaacacat
    48001 gctcgtggag tgccagtttt tgcgaggaag gggagttaga gacaggaatt gcggaggcaa
    48061 tcacataatc gtggttaaac cacgaaaatg agaagagaac ctgacaatgg aattatctgt
    48121 aattttctca tattgatatt tcattaatca ttctggcatg tcgtactctc gcatttaatc
    48181 gtttttatct ggatagcact cttttgatcg gcaatgttga aataatgaaa gcggcataga
    48241 ttgaggggca ataatttgaa ctcagtcgca aaattataat taaaacggtc atgctaccta
    48301 atatatgccc gtttattgca tcgcgaaatt tgagaagaca taactcatta aatgaaaggg
    48361 atgcaaatga ataagtttat cgctgctgaa gctgcggaat gtataggctg tcatgcttgt
    48421 gaaattgcct gtgcggtggc acacaatcaa gaaaactggc cgctgagtca cagtgacttt
    48481 agaccgcgta tccacgttgt agggaaaggc caggctgcga atccggtggc ctgccatcac
    48541 tgcaacaatg ccccttgcgt tacggcttgt ccggttaatg ctctgacttt ccagtccgat
    48601 agcgtacaac tggacgagca aaaatgtatt ggttgtaaaa gatgcgcaat cgcttgcccc
    48661 tttggcgtcg ttgagatggt cgatacgatt gcgcagaaat gcgacctttg taaccagcgc
    48721 agttccggca cgcaagcctg tattgaagtc tgcccaacac aggcgttacg actgatggac
    48781 gataaagggt tacagcagat aaaggtggcc cgccagcgca aaacggcagc aggaaaagcg
    48841 tcatcagacg ctcagccatc tcgcagtgca gcgttgctcc ccgttaactc gcgtaaaggc
    48901 gcagataaaa tttcagcgag tgaacggaaa acccactttg gcgaaatata ttgcgggctg
    48961 gatccacaac aagcgactta tgagagtgac cgctgtgttt attgtgccga aaaagctaac
    49021 tgcaactggc attgcccgct gcataacgct attccggatt acatccgtct ggtacaggaa
    49081 ggaaagatta ttgaagcggc agaactttgc caccagacca gttccttacc agaaatctgc
    49141 ggcagggtat gtccacagga ccgtctttgt gaaggcgcat gtactttgaa agatcactct
    49201 ggcgcagtca ctatcggtaa tctggaacgc tacatcaccg ataccgcgct ggcgatgggc
    49261 tggtgtcccg atgtcagcaa agttgttccc cgtagcgaaa aagtggcggt gattggcgct
    49321 gggccagcag ggttagggtg tgctgatatt ctggcgcgcg caggagttca ggttgatgtc
    49381 tttgatcgcc atccagaaat tggcggtatg ctgacttttg gcatacctcc tttcaaactc
    49441 gataaaacgg tattaagcca gcggcgagag atattcaccg caatgggaat cgactttcat
    49501 cttaactgtg aaattggccg cgatattacc tttagcgatt taacttctga atatgatgca
    49561 gttttcatcg gcgtggggac ttacgggatg atgcgagctg atctgccgca tgaagatgcg
    49621 cccggtgtca ttcaggctct accgttcttg actgcccata cccgccagct catggggttg
    49681 ccggagtctg aagagtatcc gctgacggac gtggaaggta agcgagtcgt ggtattgggc
    49741 ggtggcgata cgacaatgga ttgtttgcgg acttccatcc gcctcaatgc cgccagcgtg
    49801 acctgcgcgt atcgtcgtga tgaagtcagt atgccgggct cgcgcaaaga ggtggtcaat
    49861 gcgcgcgagg aaggtgtcga gtttcaattc aatgttcagc cgcaatatat cgcttgtgac
    49921 gaagatgggc gtttaactgc ggtgggcctg attcgtaccg ccatgggtga gccggggccg
    49981 gatggtcgcc gtcgtcctcg tccggttgcg ggttcagagt ttgaattgcc cgcagatgtt
    50041 ctcattatgg cctttggttt ccaggcgcat gccatgccgt ggttgcaggg cagcggaatt
    50101 aaactcgata aatggggcct gattcaaacc ggcgacgttg ggtatttacc tacccagacg
    50161 catctgaaaa aagtctttgc tggtggggat gcagttcatg gcgcggatct ggttgtcact
    50221 gccatggccg caggaaggca ggcggcgcgc gatatgttaa ctctgtttga tacgaaggca
    50281 tcgtgatgaa atcgttaatt atcgttaatc cggctgactg tattggctgc cgtacttgtg
    50341 aagtggcctg tgtggtcgct cacccttcag aacaggagtt gaatgccgat gtctttttac
    50401 cccggctgaa ggtacagcga ctggacagca tcagtgcgcc ggtgatgtgc catcagtgtg
    50461 aaaacgcccc ttgtgttggc gcttgccccg tgggggcgct gacgatgggc gagcaggtgg
    50521 tgcaggccaa ttctgcccgt tgtattggct gtcagagctg cgtcagtgcg tgcccgtttg
    50581 ggatgatcac cattcagtca ttgccggggg atacccggca acaaatcgtg aaatgcgatc
    50641 tctgtgaaca gcgggaagag gggccagcct gcgttgaatc ttgcccaaca caggcgctgc
    50701 agttgcttac tgaaagagaa ctcaggcgag tccgccagca gcgtattgtt gccagcggtg
    50761 agaatcctct ctgaaagaaa tctccgcgct gaaaggcgcg gagaatctat tagattgccc
    50821 aaccacccgc gtaaaatgcg accagtgcgg cagtaatgat caccgtacca atattcagct
    50881 tttgccattc gcgtgcaaag acgcggccta cgaccagtgt cacaaagccc agcataatac
    50941 cggtaacgat attacaagtc agaacgatga acacggcaca caccaggcca gccatagcgt
    51001 caataaaatc attgaaatcc agcttcgaga cgttacttaa catcagcaaa cctacgtaca
    51061 tcagagcggg tgcagtggcg taaccaggga tcaaaaatga cagcggtgat aagaacagaa
    51121 tcagcaggaa taacgccccc actacggttg cggttaaacc tgttttaccc ccggcggcgg
    51181 ttcctgccgc tgattcgata taaaccgctg cgggcgctgc gcccaccagg ccggagaata
    51241 ttgaacttac tgagtcactg gtcagggctt tgccgccgtt gatgatctgg ttgtctttat
    51301 ccaacaaatt cgcctgaccg gcgacggcac ggatggtgcc agtagcgtcg aacactgcgg
    51361 tcatcaccaa tgccagtaca ctcggaagta cagttggctg gagtgcaccc ataatatcga
    51421 ggctgaaaat cagagactta ccatcttcgc cagtcaggct tggcatcgcc accagaccgt
    51481 ggtatttcac cgctgggtca aagattaagc cgatgatcga aattgcaata atcaccaaca
    51541 agatcccgcc gggtacgcga cacttctcca ggccgaagat gaccgccagc cccagcaagc
    51601 tcatcatcac cgggaaggag gtaaacgcac cgagcgcaac tggcaagcct tcaatcgggt
    51661 ttttgataac catacccaca ccgttagcag caatcagcag cagaaacagc ccgataccga
    51721 tacctgtacc gtgagcgata cccatcggca aattacgtaa gatccaggta cgcacaccgg
    51781 ttacggaaat ggcggtgaag atgacgccca tcagaaatac cgcgcccagt gcgacaggaa
    51841 cgctaatttg ttgcccgagt accagactga atgcggtaaa cgccgtcaag gaaatcgcgc
    51901 aaccaatcgc cattggcaaa ttagcccata atcccatcag caacgagccg aagcccgcga
    51961 ccagacaggt ggcaacaaac acagctgcgg gaggaaaacc tgctttgccc agcattcccg
    52021 gaacgacgat aacggaataa accatggcca gaaaggtcgt taagccagcc agtacttcct
    52081 gacgaacggt actgccacga gcggtaattt taaaataatt atccagcgcg ccctgtggct
    52141 ttggtgcgtc cggtgtttgt aggatgtctc cagacataat gatgtcctca cgaggatgtt
    52201 aaagttttat tattagttgc gttcgtacac cagacgacca tcaacgtagg tgcggtagat
    52261 cgaacggtca tcgcccaacg tcatcatcac gaacaatttg tcgactaaag aaacagagtt
    52321 gtcatagcgc agctgctgta gcggagtggc ggtgggttcc atcaccacga aatcagcctc
    52381 tttgccaggt aaaaagttgc caatcaaatc gtcaaggccc agagatttcg ctccgccgag
    52441 cgtggccagg taaaacgctt catatgccga gaggcgatag ccttgtaatt gcaatacttt
    52501 gtaggcttcg ttcagcgttt gcagcatgtt gaaagtggtt ccggcaccga tatccgttcc
    52561 catgcccact ttaactttct tctgccatgc ttttttcaag ttgaataagc cgctgccgag
    52621 gtaaaggttg gaggtcggac agaaagcaat gctggatttg gtttcgctga gacgatccca
    52681 ctctttttct tcgagatgga cgcagtgagc aaagacacag tttttaccgg tcaggccgta
    52741 ctgatggtaa acatccagat aaccatcatg gtcaggataa agcgatttca cccaggcaat
    52801 ttcatctttg ttttcacaga gatgggtatg tacccacgta tccggatatt cttctttcag
    52861 gcgttgcgcc atcgccatct gttcaggaga tgaggtcggg gcgaagcgtg gcgtaatcgc
    52921 atatagcaga cgaccatttt tgtgccagcg ttcaatcagt tctttgcttt ggtgatagct
    52981 gctttcggca gtgtcgagca gataatccgg tgcgttacgg tccatcatca ccttaccggc
    53041 aatcatacgc atattgatat gactggcggc ttcaaacagc gcatcaacgg attgcggatg
    53101 aacagtgcca aacaccagcg ccgtggtggt tccgttacgt aaaagctgct tgatgaagaa
    53161 cgccgacatt tcgcgggcgt actctaaatc ctcataacgc cgttcagtag ggaaggtgtg
    53221 tttattcaac cactccagca attgctcacc ataggccccc accatttcac tttgcggata
    53281 atggatatgt gtatcgacaa agcccggtac tatcagtttg ccgcgatagt cgcgcacgcg
    53341 aatggtgtca ggaatttgat gctttccgtt ttcccattcg ccaaaccatt ccacttttcc
    53401 ctgtttaatg agtaataaac catcctcaat aaaccgcagc gcagaggcaa tctcttccgg
    53461 gttatcgacc gtacgggtga catcaataaa actgcctcgt accgctttta acgtgtgttc
    53521 tcctgacatc attgactcct ttactgttaa tccatctctt ccgctgaggt aataccaggc
    53581 agaacgtttt cctgtcggta gccaccaggg aggataatat ttaataaaat cgcagttaac
    53641 ccgccagcac aaatagggtt ttcaactaat acataaatag aggctggcaa tattttaaaa
    53701 atttcgggat cgtaggagac gccaagccct aaacctaaag aagtggcgac aataagtgtt
    53761 tcacgccgct ttaatccgtt ggtgatgatg atgcgaatcc cagcgatggc aatcatggaa
    53821 aacatcaacg tcattgcgcc tcccagaact gccgagggaa tggtcgtgaa gaagccgcca
    53881 atcatcggaa ataagccgag gataaccagc attaccgcga tggttcgccc gacataacgt
    53941 gaagcgacgc cagtcatctg aataacccca ttattttgcg caaacgtggt taatggtaat
    54001 gaaccgacag cggaggcgat aacagaaacc agaccatctg ccagcacgcc gcctttcagt
    54061 cgggactgat actcttcccc ctgaatgggg cggcgggaaa ccattgccgt ggcggtgata
    54121 tcgccgactg cttccagcac gctaagcaga taaatcgtgc caaccaccag gaactgatgg
    54181 aagctaaaac taaagccgta tttgaacgga tgcgggatgg tgattaacgg caaattgcgc
    54241 atactgctga aatccaccat gcccaggcat aacgatgcga tatagccgac acatagccca
    54301 atggcgatcc ctcccatgcg tagcaacgga ctgcgacagc agttaaagcc gatcaccaca
    54361 attaaaacca ataaaccaac gccgagatgt tcgtaattgc cgaacgtacc gctgcttttg
    54421 gctgcaaatc ctccaccaaa atcgataatg ccgactttaa tcaggcttaa gccgatcatc
    54481 agtaccacaa taccgctgac ggtaggcgta ataacccgac gtaaataagg caagataaat
    54541 gaagatccga caaccagaaa tgcgccaacg aaggagacgc cgagaagcga cgacatgatt
    54601 aactcttcgt gaaaaccgtc gcttttcatg ctgctgccca gcgcaatcat gaccgtaaca
    54661 aatgaaaaat tgactgactg aattgagagt aggccagaac cgacgatgcc gtagcggttt
    54721 acttgtaacc aggtaccaat accagaggcg atcatcgcca tagaaacaag ataggcagtt
    54781 gtttcagcgg aaagctgtaa ggccgcaccc acgattaacg cgggggttac catcggaacg
    54841 aaaattgcca acagatgggt aatggcacca acgagagcct gatgaaaggg agggcgatcc
    54901 tccagttcaa atataaggtc agaacctgca tggtttatat cagacatccc ttcactcctt
    54961 ttaaagtaaa tttgatcatc tgaattagta aaataaattc ccacgaattt ggggcgcagg
    55021 acgccttgag aacggcttat ctcattttcg cagtcactat ttagtcatcg ttcaaacaga
    55081 gaaacaggcc gccgaagcgg ccatttatta tttatatttt ttccagcgca gtgagtattt
    55141 tctccggtgt gaaatgccat tcgcgtaacc agatgccgca tgcatcgtga attgcggtag
    55201 caatcgccgg agctgcgcca tttacaccga tttccgagat cgatttcgcc ccgaacgggc
    55261 cgactttatc gtcgctcggc accagcacag cgcggaaatc acgcggaatg tcaccaattt
    55321 tcggtgcgcc gtaactgcgt aaatcacgcg ttaacgggtg accttcggcg tcataaatga
    55381 tctcttcgct catactgtgg ccgatagcac gcagggtggc accgtagatt tgtcccaacg
    55441 ctaactctgg attgaccggt gtaccgcagt ccagcaaggc gtagaattta tccaggcgga
    55501 tttcacccgt acgcgtgttg acggcaactt cagcgaagtt tgcgccatac gggaaggcga
    55561 aatcaggcgt gatataactg ccagttccca ccagtgaacc aaagccggtg ccggtttcgc
    55621 ctttatgggc aatatccccg aaactaactt cgcctttctt gccgcgcacg acgcccggcg
    55681 ttgccagttg aacatctgcc actggctcac ccaacatttg cgcgccgtgg aacagaattt
    55741 tctcccgtag attttccgct gccaaacgcg ccgcgttacc cgagaagcaa gtaccggacg
    55801 aggcatatgc gcctttatca aacaacgcat gatcggtatc accggagata acatgcacgt
    55861 cctgcggtgg gcagtgcagc acttctgctg ccagtttcgt cactacagta tccagaccag
    55921 taccaatatc cgcaccgcca gaatgaacga taaaggtgcc gtccgattcc agtttgatca
    55981 tgcagttagc ctgatcgata tccgggatac ccgatttctg catgataatg gcgacgccgc
    56041 gaccgatgtg ccagtcacca ttttgcggtt tcggtgacga ccattggatc atctcgcgac
    56101 cctgacgcag gatctcttcc agtgcacagc tggcggcgga aggaacggag gtcggcgctt
    56161 taccttcacc gattgcaccg agaattttca gctcttgccc ttcgtgtacc cggttacgtt
    56221 cgataatttc cagttggtcg atctgtaact gttcagccag ttccgctaat gccatggtga
    56281 tagcgaagtt acctttcggt gcgccataac cctgataagc accatttggg caaatgttgc
    56341 tgtagtaggt ggtgacctgg aaatcgacgt tatcgcacgg atataacggc agcgacagcg
    56401 ccggtccgtt acacggtacg gtgagtgagt ggttgccgta agggccagtg ttggcgcgga
    56461 aatccatctt cactgccgtc aggcgaccat cttttttcgc tcccagtttg acggtgactt
    56521 tcgcgacgtg acgagaggtg ttagcaataa actcttcttc acgggtgtag cggaacagta
    56581 ccggacgccc ggtcacgcag gttgcccagg cgcacacttc ttccagcagg atgtcctgtt
    56641 tggaaccaaa accgccgcca actcgctctt taatgacatg aactttatgc tgtttcatgc
    56701 ccacgaggcg cgcgacctgg cggcgtaagt gccatggtac ctgggtggag gcgtggataa
    56761 ccagacgatc gccgtccata cgagtaaagc agatatgtgt ttcagtcggg cactgctgcg
    56821 cttgcgttga gttataggtt cgctcaatga tcacatcggc atcggcaaag cctttgtcca
    56881 tatcgccaat atgaccatga atactggcgg cgatattttt gcgagggcga gaaccgatcg
    56941 ggaagttgat gatcatatgc tcgccgcgct gggctgcatg gctgttatcg tcttccagag
    57001 tatctggcgc accagcaaca tacaccaccg gttcatcgtg cacgacaggc gcatcttccg
    57061 ccattgcttc gtcgatcgac attaccggct taagcacttc atattcaacg tcgatgagct
    57121 tcagtgcttc gagcgcaatt tcttcacttt ctgcgacgac cgcagcaaca cgatcgccga
    57181 cgtgacgcat tttcttgccg aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct
    57241 gaccacccgg ggtatagtag atatccgggc aattcaggtg agtaataacg tgaacgacgc
    57301 ccggtaaggc ttcagctttg ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc
    57361 tacgtaacat tttaatgacg caggcgtcag ccgttacgcg gtcttcaaca tagcagggtt
    57421 tcgcctgcac cattttcgcg gcatcagttt taggataatg cttaccaatg acttctaggt
    57481 cgtcacggaa agtcggagcg atatcaatgg tggcctgcgg attattttta cgtgcaaccg
    57541 ccagttcaat gacctgatag tattgctgcc agccagcatc gcgactgaac aaaccagaaa
    57601 gcgcgtcgtc gatctcttcg cgggtaggtg cggcgatgcg atcgagaaga tcggtgataa
    57661 tcagagctgc agctggatcg ttataaccag actgcaccac gccaacatca accattgcct
    57721 gttgaaccag acttaactcg ttccatttgc ccagagattc tgcggtacga atatctgcct
    57781 tctctaactg tgcggcaata agcaaggacg cgttaacgat attaccgtta aagattattg
    57841 cgtcagaacc ggcaaacccg aaaccatcat cactgttgcg tacagagtgc attcccatgt
    57901 taaacaacag cttttgcacg ttttcgcctg gattaacggt tagctcctga ggcgcgccat
    57961 ttaaagtaaa gtggatgatc atacggcttc ctccccagcc tgttggcagt cggcatacag
    58021 gtcggctacc agaactcccg tgatatagcg tttataggcc acgctgcccc gcaaatcttc
    58081 ctgcgggaaa atggcgttgg cgacagcctg ttcaagtgca ttgccttcca gattttgttt
    58141 ttcgacatca tgcagacgca gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc
    58201 gtcgtggtct gtcattgcaa cggctgccgt cacgacggtt aaacctgcct gagaacggct
    58261 aattttgcgg gtcgcacagg tgcgatacgg atctttaatg ataatttcgg ttagcaggcg
    58321 atcgcatggg caggccaggt agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag
    58381 ttcagcatcc agtgccaaca ggacgggaag cagcaccgac tcttcctggc gggcggcgat
    58441 ttcaccacca atggtcgact gattacgaac atggcgtgag taaacaaaac cgagggcttc
    58501 acgcagcgct gcaggaataa atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc
    58561 aatccgcagt gcaccgttat cccagtcaac ccagtccagt tccagatcct gtaaggaaat
    58621 ggcaatcttt ttatcggtac gggttggtgt agcattgagt ttgctgcccc cggcgaacca
    58681 gacggcttca tcctggtagc ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct
    58741 gaaaaattgt tcaatcatct tctttcctta tttaaccctc ttccctgcgg gaagagggca
    58801 tctgtcattt atgccagcgc atccatccga cgccacatac tggcagcggc ttttctggct
    58861 tgtgcataaa tggaatcgca atcgaagtta aactgacggt cttcatagac catcacacca
    58921 ttgaccatca cgctgtgaac gctgcctgag cccataccga aagcgatatg cccggcgata
    58981 ttgtctgcca gcagcggcgt cggcgagttg taatcgcaaa tggttaaatc agctttgtaa
    59041 ccggcttcca gaagcccaaa tttcgcgcca aagttgcggc tcatcagttc gttaccgtta
    59101 gtcagggctt tggcaaaact gtcaggccac agcggaccac ccgcatcgcg atgtttaaag
    59161 aaggcaaatt tcatctcttc aaacatgtcc gaaccaatgc cgtctgttcc caacgccaga
    59221 ttgcggatgt cgctaaggtg atggttgtag ccgacatggt tgttcatgtt tgaacgggcg
    59281 ttatgcacca ggaacgcatc gcgctgattg agtagggtga tgtcatcttt cgacaagtac
    59341 agcccatgag cgaccagcgt tttgctgtcg atgagatcga attgcgccag tcgtgccagc
    59401 aggtctttgc cgtaccagtg gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga
    59461 atatgcaaac cacggcctgt ggctttcacg gcttcacgca gcatctccag accggcatcc
    59521 ggcacggtaa acggcgcgtg agcaccgata tgtgcttcca ccagatacgg ctcgcttgtc
    59581 gctttcttcg cctcatcaat caaacgggcg aaacggatgt tttcttctac accttcctgc
    59641 aactctttga tgccgttgtt acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca
    59701 acttttaaaa atgcgtcgcg caatgtggag agcgacccgc cgatatacgc cggagaggca
    59761 tggtgatcga taaccgatgt acatccgctc ttaatcgctt ccagggaaca aatcagtccg
    59821 ctgtaataga gcgactcttc atcaagggcg cgatcgagcc gccaccagag atttttcagc
    59881 gttgagatga aatccgggca aggggcgatg tttgccataa ttccgcggga aagccccgag
    59941 taaaaatggt tgtgcgagca gacaatccct ggcatcacaa tccggccatg catctctttg
    60001 aagctggcgt cggggtagcg ttgcgtcagg gcatcgccga tagcgacaat cacatcgttt
    60061 tcgatggcga tatcaacgcc ttcctgcact tttgccgggt gtagctgtac cgcagtgaca
    60121 ttcttcagaa tcaacatgat tacacctcca cgcggcccag cagataatga tgatgctggt
    60181 ggacatggct gatgatgcgg cacatatcgt tcagctccgg tggtacgttg ttaaactgac
    60241 ctttgctgtc gatgtttaac acccagcttt ggttattcag acgtactcgt acccggcaat
    60301 cttccacaag gaagcctggg ttgctgctgt tatcaaagtc ttgcgccagg ctgaagacgg
    60361 tgattttgtc tttgtacggt ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt
    60421 cgttacagta agcgtcgagg tgcagcgtct ggaaacggtt ctggaagcct gggaccgcaa
    60481 tggagacgtt ggcgcggttc gggcagacat ccacacactt gctgcaaacg tagttacatt
    60541 cgaggcagcg agcggcttcc tgggcgacaa acgcgtcacg atcgtcactg ttcaccagcg
    60601 tgatagagat atcgccttta cgttgataga tttccgctgg attgacgttg ttccagtatt
    60661 tatcgttctg gtgggaacgg atattttccc gactaaggat ggcatcggtc gcccgacgcg
    60721 cggttccgac agcagcgaca atggaggatg gtccgcgctg tacgtcgccg atcataaaga
    60781 cgtcagtcag acgagtttcg ccattatggt cgacgtctgg ccagccgttt ttgtccagcg
    60841 gcacgcccat cgcattcagg gcttcagtat cctgctgttc accaatggcg gtgatcaggc
    60901 tgtctacaag cagtgttact gtttcattgg tttcaaccgg acgacgacga cctttctcat
    60961 ccggttcgcc aagcgacata acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg
    61021 gattattcag gaaacggaac tctacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc
    61081 atgcgggcat ctcttgtagt gaacgacggt aaacgatcgt tgctttttct acgcctggaa
    61141 cgcgtaacgc cgcacgagcg cagtccattg cggtgttacc cgccccgaca acgaccacat
    61201 gtttgcccag cttgagcgct gtacccttgt tgtattcacg caggaagggg agtgatttcc
    61261 agacattttg gttgtcgccc gccagtttca caccgctatt tttatcagtg ccggtggcaa
    61321 tcagaacata gtggaagccc tgatttttta actgctcaat ggttaaatcg ggtgagcagc
    61381 catactcaaa tttcacgccg tgagcggcaa caaaatcgat atcgtgctga attaactctg
    61441 caggaatacg gaactgagga atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt
    61501 caaacagcgt aaccggatgg cccgctctgg caaggaagta acctgctgcc agacccgccg
    61561 gacctgcacc aatcacggca accggatggc gtgaaccaga accggctggt ttgtgccagc
    61621 gttgcttata ttcatcccaa cctttttcca gcgcgacttt tttcagttcg cggatattca
    61681 gcgcactgtc gtaatccagg cgggtacagt tgtattggca ctggtgatcg caaatatgac
    61741 cggtaatggc gggcagagcg ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt
    61801 gttcgccaag cagacggatg tattccggaa tatcttgctt gatagcgcag gcagtaacac
    61861 agggggcaac gtagcagtcg gtcagcggca ggtcttctgc cacttcaata cgctcttctg
    61921 gcttccagtg tttctgggtg tattccatgg ttaacgcatc tgctgccagt ctgttcagtc
    61981 gttcgacgtc aacatggtca agtccccagg cgtcggagcc ttccagctcg cgcatgcagg
    62041 cacttaagcg cagatagccg ccaggtttca gcaggtcggt tgccatagta atagggcgaa
    62101 tacctgtatc aaaaatatcg cggatagtca gctgactggc accaccggaa taagaaatgg
    62161 gcagtttgcc gtcaaaggcg cgagagagaa ctgctgcaac attgatggag agcgggaaca
    62221 gcgcacggcc tgacatatac atctcttcac cgggcagtgc gcctttattg ttgatggtgc
    62281 cgagagtgtt agtcagtttt acgccaaagc cgagtgattt ttcttttgcc agtgccatca
    62341 ggcgttccag catttccagc gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt
    62401 ttaagcctat gtaaccgaaa ccgcagatat cgaggatctc acgaacacgc gcgtatccca
    62461 gtaaggtcgg gttaagtttc acaaaggtgt tgagcccttt ttcttccagc atgtagcggc
    62521 aaatggcttc gatttcatgc ggaggacagc cgtgcatggt ggagagggtg acgccatgca
    62581 ccatactggt ggggatgcga gcgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc
    62641 cgtggcgagc taaaaatgcg tcatcctgga gtaatttatt cagcgtatcg cgatattgcg
    62701 cgaatttcgg atggtcagat gcgtccatca tattgtcgat gaactgttgc attggcggtt
    62761 gcttaatacc ttcgaggttg taaccgacgc tcatattaaa gatgaacgat ttaccggaat
    62821 cagaaggttg gaacatcgct tcgagaaggt gcagggcaaa ccaggctttg aggtattcat
    62881 cccaggcttt aagcagggta aattcggtag accattcggt gttaaagcac tcgtcttcgg
    62941 catcaataca gggcttttcc agctccaggc ggtcaagaat ttggacggtt tttagttcga
    63001 tgaaacgtcc gccagtcagc caggaagtga caatattttg cgcgagctgc gtgtgcgggc
    63061 cagcggcagg gccgacggga gtggcacagg tttcaccgaa gacgctaaca gttttacctt
    63121 ttacaggtga gtaaaactgt tgctcgggaa taccaaagat tgagcgttgt tgttggtatt
    63181 catcaaatat gcgcgtcaaa agttcctcaa acggaatggg acgcataata tcccccataa
    63241 ccctctcctt agcttataca gatatcattg agtgaccggt gttgagaaat ccggtggttt
    63301 agggggatag agcaacaatc gcaccactct tgccaggttt ggcgggaaat ataaaaaaat
    63361 gtgaggcggc tcgaaactta agggcttttt gtgaatgaac tcacaggtgc taaatgagaa
    63421 ttaaaatcac tcctgaaaat tattattgcc agcccattta tcaatttttc tcttttctca
    63481 aaatcagtga ttccttatca ttatgataat gagtgccgga aggattaaat atttgaacgc
    63541 aatcgtttaa ctacgttttc taaatttcag tatacctttc cttcgctgta ataaagtcgt
    63601 ccggagtatc aatatctaaa attatttctg cattttctat ttcgacggaa taatgatctc
    63661 ccataaggag tgcctgacgc atattggtga cattgggtcg ttggattgcc tgcatcaggc
    63721 atggttttga cactaaaatc ggatggccgg ggatgccatt atggagtggc agtattgcgc
    63781 catcatttcg taacgaccag atttttctaa aaatatcgat ggtgagggtt ggcatgtcac
    63841 cgtgggtgag aaaacaatgt tctgtttgca ctgcgggtac tgcggccttg acggatgtta
    63901 gtaaaccctg cgcataatct gggttgtgaa taatagtgat attgctctgg ttcgcataac
    63961 gttcgtgcag ttcattacca cgatagccgg tgactaaaat aattcggcta caaaactgca
    64021 acgcattttt gatacttgta tcaagaattg ttccctgttc ccagggtaac atcattttcc
    64081 attgccccat tcttgatgat aatccggcag cggtaattat acagtcgatg gctgacatag
    64141 tggttccatt atttataggg gctaataacg cctgataatt gcgttacttc ctgatggatt
    64201 aagttgggga taatttatcc cagagaggtc ataaagactc gtgaaaagta tagttgaccc
    64261 atcggcatta gtcattgatt taggtgcgca gaaacgtccc actgtgattt ctgttgttgg
    64321 tgcagggggg aaaaccagct tgcttttttg gctggcagaa ctgctccagg ctagcggcag
    64381 gcgtgtgtta atcacgacga cgacacatat gtttatgcca acgtctcact ggcccgtggt
    64441 tttctgtcgt gatcccgcca tgcttcctca tgcgtctctt acatccccca tttcattttg
    64501 ttttcacagc tggaaagcga accagggaaa agtgcaggga tttacgccag aagcgattga
    64561 tgcactggtg caacgaccag agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg
    64621 aatgccgtta aaagcgcctg atgagcacga accttgcata cccaaaagca gttgctgcgt
    64681 gattgctgtg atgggaggac atactttggg cgcgaaagtg agcacagaaa atgtccatcg
    64741 ctggtcgcag tttgctgata ttaccgggtt aacacctgat gcaaccttgc aactgagcga
    64801 tctcgttgcg ctggttcgcc accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg
    64861 gcgagtctgg ttcattaacc gtttttctca atgtgagaat gcgattgcac aaagcgagct
    64921 ccttcaaccg ctgcaacaac atgacgtaga ggcaatctgg ctgggcgata tacaagaaca
    64981 tcctgcaatc gcgcgcagat ttgtgaatta gcggcccggg tattccgggt tactgaaagg
    65041 atatccattg aggttggtat gaatattttc acagaggctg caaaactcga agagcaaaat
    65101 tgtccgtttg cgatggcgca aattgttgat agccgaggct cgactccccg ccattctgca
    65161 caaatgttag tgcgcgccga tggttctatc gtcggtacaa ttggtggcgg aatggttgag
    65221 cggaaggtga ttgaagagtc gcttcaggca ttgcaggaac gtaagccgcg attattccat
    65281 ggacgtatgg ctcgtaacgg tgcggatgct gtcgggtcag attgtggagg tgcaatgtca
    65341 gtatttatca gcgtccatgg tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc
    65401 aaccgggcga tagcccagag tgcggcgcta ttaggatttg atattgccgt tgccgatatt
    65461 tatcgcgaaa gcctcaatcc tgaactattc ccaccatcaa ccacgcttct ccatgctgag
    65521 tcgtttggtg cggcagtgga agcactggat attcgccctg ataattttgt cctgattgcc
    65581 acgaataatc aggatcgtga agccctcgac aaactcattg aacagcccat tgcatggttg
    65641 gggttgctgg caagtcgtcg caaggttcag cttttcctgc gtcaattgcg tgagaaaggc
    65701 gtggctgaag aacatattgc ccgtttacat gcgcccgttg gttacaacat aggtgcggaa
    65761 acgccgcagg agatcgccat cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg
    65821 ccgggtgggc tgatgatgaa accttctcat ccttccggac accagctggt ggtgattcgc
    65881 ggtgcgggag atatcgccag tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg
    65941 atcatgctgg aagtggaaaa accgacagtg attcgttgta ccgtggcgtt tgcccaggcc
    66001 gtgttcgatg gcgaaatgac ggtcgaaggc gtcactgctc gcctggcaac cagctctgcg
    66061 gaagcgatga aacttaccga acgcggattc attcctgtga tggtagatcc cgcctgttca
    66121 ttgcttgatg aactgaaacc gctttgcgtg gtggacgcta ttctggcgaa acagaatttg
    66181 ggaacgcggg cagatatggc accagtaaca atcgcgcttg ggccgggctt tactgctggg
    66241 aaggattgtc atgcggtaat tgaaacaaat cgcgggcact ggctcggtca ggtgatttac
    66301 tctggttgtg cgcaggagaa taccggtgtt cctggcaata ttatggggca taccacccga
    66361 cgggtgatcc gtgctcctgc tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg
    66421 gtgaaagagg gcgatgtgat tgcctggatt ggtgagcatg aaattaaagc accgttgacg
    66481 gggatggtgc gtggcttgtt gaacgacggc ctggcagtgg ttggtggttt taaaattggt
    66541 gatatcgatc ctcgtggtga aaccgctgat ttcaccagcg tttctgataa agcccgggcg
    66601 attggcggcg gcgtacttga ggcgttaatg atgttgatgc atcagggcgt gaaagcgaca
    66661 aaagaagtgc tggaagtggc ttaaatgaaa atggccggat ggtattaccc acccggcctg
    66721 agactgatga caaacgccaa attgcctgat gcgctacgct tatcaggcct acaagtgcat
    66781 tgcaatctat tgaatttgcg atgttttgta ggacggataa ggcgctcgcg ccgcatccgg
    66841 catcaacaaa gcgcacttac ttgacaatct ttggccggat ggtattaccc acccggcctt
    66901 taaattactg acaaatcacc gttcctgttt tgccttcaat cccttctttc gctttgctca
    66961 atacggtaat cagtgcttca cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca
    67021 ctttaggcag catagaacct ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac
    67081 ttaatgacag gcgatcgagc cactgttcat tctctttacc gaagttaatg gccacttttt
    67141 ctaccgcagt cagaatgatc agcatatcgg catcaatcat ttctgctaaa cgggcgctgg
    67201 cccagtcttt atcgataacc gcgctggcac cacgcagatg gttaccttca cgaataacag
    67261 ggataccacc accgccaacg gtgatcacca cctgacctgc atctaccaga gctttaaccg
    67321 tttctttttc aatgatatca accggttttg gcgaggcaac aacacggcga tagccgcgac
    67381 cagcatcttc tttcagagtg taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa
    67441 agaacgaacc gatcggcttg gtcgggttga ggaatgccgg atcgttagcg tcgacttcga
    67501 cctgagtaac cagcgttgct acaggtttat taatgccccg agaaagcagt tcttctctta
    67561 aagcgttttg tagatcgtaa ccaatataac cctggcttaa tgcaacacag acagacatcg
    67621 gcagcattgg cgagtgcgct tcagtttttg cggcagcttc aaaggcctgg ttaatcatgc
    67681 ccacctgcgg cccgttacca tgagtgacga taacttcatg tccctgggca attaaatcaa
    67741 caattgcctg agaagtaatt tttaccgctt tcatttgccc ggccaggtca tcgcccagcg
    67801 cattcccgcc cagggcgaga acaattttct tactcatttt tcctcactca tcattcttga
    67861 ttgtgtatag atccctggca gtggttagag cacgggaggg acaaacggtt tgcgtcgcag
    67921 gaaacgacct cgcccggctt tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg
    67981 ggataaggtt ctgacaatcg cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc
    68041 gttgtcgtgg agatggcgat gctgaatttg ttggctctga cgtgggtcga taatcaccac
    68101 gtcgccgtcg gaaccgggcg ctaataatcc tttttgcggc cacaggccaa acaacctggc
    68161 gggcattgcg ctggttaatt caacaaagcg ttccggtgtt atacgtcccg tcatcacgcc
    68221 actggaaaac agtaactgca tgcggttttc cacaccgggt aagccatttg ggcagcgact
    68281 gaaatcgcct ttagaaattt gcaggcgttg agccatcgag aaggtgcagt gatcggttgc
    68341 caccacgtca atcgcaccat cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt
    68401 acgcagcggt gggctaagaa tgaacttcat gccatcttct gtatcgtaac tgcgttcgtc
    68461 caacaggaga tattgtgggc aggtttcaac ccagactggc tggtgattcg cacgggcaag
    68521 acgcagataa tccagaccta agccgttaga caggtgcacg atatagagcg gggcgttacc
    68581 ggcaatttgt gccaggttaa tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg
    68641 actcaaggca tgatagcgcg gcgcggttaa ccctgcggcg ataaattccg cccgcttgct
    68701 ggcgatagcc gcatcatttt ccgggtgcac ggtggtcagc gcgccggatt catgcaagcg
    68761 gcgtaatgcc tgcaaaacct cgtcatcgtt gagtttgtat tgataggtta aatagagttt
    68821 aaaactgctc agtccttcct cgaccatcat cgggatttcg tcgaggattg cgtgattaat
    68881 gtgctggatc acaccgtgaa agctgtaatc gatgaccgct ttatgggcgg cataaccacg
    68941 ataaacctcc agttgatggc gtaaccgaca gccgtttggg ccaaatccca tatggtcaat
    69001 aatggttgtt gtaccgccac acgcagctgc gcgggtaccg gtaaaaaaat catcacaact
    69061 gcgcgcgatg ccgacatcaa tattgaaatg cgtatggaca tccacgccgc cagggaaaac
    69121 gtaacagcca gtggcatcaa tttcttcata cgggagctgc ggcgaaatat tgttgcccaa
    69181 ctggcgaaca atcccgcttt caatcagcaa atcctgtttg gcttgtccat ctgcgttaac
    69241 gacagtgccg tttttgatca atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg
    69301 gaggggaata agtgaagagt aattactctg ttgccagcca gcttaacggg attgcagcgt
    69361 acatcgcggc acaggtcacc aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt
    69421 caggttcttt acccgggcca aagccgatca ccggaatgcc gtgacggccc atgatagata
    69481 cgccgttagt tgagaaggtc cacttatcaa caaccggcgc tttgccaaac agcccttcgt
    69541 aggcattcac cagtgcttta acggtgaagt gatcttcttc cactttccag gtcgggaagt
    69601 agcattcggt tgggtaaacc aggccagtcc aggacggacg gtcgtagttg tacatagaaa
    69661 caaccgcgtt agctttctgt actgcaggca gggcgcggat ttcgtccagc gcgccttccc
    69721 aggtttcgcc ccaggtcaga cgacggtcga ttgaaactgc gcagctgtct gctacagcgc
    69781 aacggcttgg ggatgtgaag aagatttcag aaacggtgag ggtgcctttg ccgaggaatt
    69841 cgtcataacc cagacgttgg gagagttctt gtaattcgcc aagaatcgga cccattttga
    69901 aaatggcgtt atcaccgcgt tctggtgcag aaccgtggca gctaacaccc tgaacatcaa
    69961 tacgaatttc catacgaccg cgctgaccac ggtatacctg gcagtcggtt ggttcggtac
    70021 tgaccacaaa ttccgggcga atgccggatt gttcaataat gtactgccag cacagaccgt
    70081 cgcagtcttc ttcctgcacc gtaccggtaa ccagcagggt atattcatct tccagaccga
    70141 ggtctttaat gattttaccg gcataaacca tagaggccat gccaccttcc tggtcggaag
    70201 taccgcgacc accaatcagt tcatcagttt ccatgccttc gtacggatcg aagtcccagt
    70261 ttttgatgtt gccaatgccg acggtatcga tatgagcgtc cattgccacc agacgcgggc
    70321 cgtggccgat ataaccgaga acgttgccca tcgggtcgat ttcaacttta tcgaagccga
    70381 ctttttccat ctcttcttta atacgatgta ctacgcgttt ctcgtcgcag ctttcactgg
    70441 gaatagcaac catgtcgcgc aggaagcgag tcatatccgc ctggtaatct tttgcttttt
    70501 caagaatcag tttgaatgga atattcttag ccattatttt tctccagatt ttttactccc
    70561 cgttttgcag ggagcagttc caatagggtg attaaggtgc tacagcgtgt ttgccttccc
    70621 agacaacttc gcggtagtgc ttcacgtcgg tgtcgccttc agtgctgata accagcacca
    70681 cggcatcttt gttcagcgcc agtttttcca tcaggctttg acgttgcggg tgataatgaa
    70741 ccgctgcgag aacgcccaaa ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt
    70801 tgccgtacgg attacccagc acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga
    70861 tgaattgggt ggcgcagtta cgtaggattt cccagcccag cgggttaggt tcgccacagg
    70921 ccaggcctgc catgatggtg gccatatcac cgccaacgtt gacgatgtcg cctttgacgc
    70981 cggagcgata aatacagtca gctttgtcag gttcaacaat aatgctgtgc agattttgcg
    71041 ggctatagac gtcgaccaga taacccagca caccaccggc cattgctccg acaccggctt
    71101 gcagcagaac gtgcgtcggg gttacgccca tttcacgcat ttgctcgacg gcttcatctg
    71161 ccagggttgc gtagccttgc atgatccagg ttgggatttt ggtgtaacct tcccatgccg
    71221 tgtcctgtac cacttcccag ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat
    71281 cgtcatagtt catatccgtg acgatgcact cggcaccgag gttcagaatg gcgtcaacgc
    71341 gttcctgagc agaacctttc ggcatgtaaa tcaccgcatt ctgtccgagt tgctgtgctg
    71401 cccacgccac accgcgcccg tggttgccgt cggtggtggt cgcgaaagtc attttttcgc
    71461 tgatggcatt tttcaggtgc tcaaatgaca gcgtttcgat atcaagatga tatttttcac
    71521 acaataattg agcgatggcg tacgcaccgc caagcatttt gaaggcgttc agaccgaatc
    71581 gttttgattc gtctttgacg agaattttct tcacaccaaa aaggtttgcg agatcgtcca
    71641 gcgcacaaag cggtgttggg cgataaccag ctattttctg gtggaactgg cgcgccagtt
    71701 tggcctggct ttgcgaaaag agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga
    71761 tatcaatctt caatgagaaa acggacatat cctatccttt tggcctctgg aaatagaggg
    71821 ccgaccagct gatcggcccg aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt
    71881 gctcgagcag tgcacctggt ttggcgtatt tacgggacag gatcatcgcg gcgatgatat
    71941 aaggcttcca gctggcttct ttgtaggtag cgatacggta tttttcgaat acgccttcag
    72001 tcacttcacc ttctttacag gatacgccgc tgatatcagc tggcaggcag tgcatgtaca
    72061 gggcttcgcc atcacgggtc agttccatca tctcttcagt acaatgccag tctttgtgtt
    72121 gcgcgttctg tgccagacac tgtttttcca gtgcttttaa gccttcgtga tcgttcgcac
    72181 gcagcaattc agtacgctct tccatcactt tgtaaggtgc ccatgacttc ggataaacga
    72241 tgtctgcgtc tttgaaggct tcttccatgc tggtgacctg acggaagcta ccaccggagg
    72301 ctttagcatt gtttttcgcc acttcaacca catccgggat caggtcgtag ccttccggat
    72361 gggccagggt gacatccata ccgaagcgag tcatcagacc gatgatgcct tgtggtacag
    72421 agagcggttt gccatagctt ggagagtagg cccaggtcat ggcgattttt ttacctttca
    72481 ggttttccag tgaaccaaag tgttcacgta accacgcgag gtcagccatt gactgagtcg
    72541 ggtggtcaat atcgcattgc aggttcacta aagccggacg ctgtggcagt acaccctgct
    72601 tgtaaccgtc atcaagtgca gcgccaactt cacgcatata ggcgttgcct gcgcccagat
    72661 acatatcgtc gcgaatacca atagcgtcgg cgcagaagga gatcatattg gcggtttcac
    72721 gcacggtttc gccgtgagcg atttgtgatt tgccttcgtc gagatcttgt tgtgcaaggc
    72781 cgagcaggtt aagcgcggaa gcataagaga agcgggtacg ggtggagttg tcgcggaata
    72841 cggaaatacc taatccacta ttaaagactt tggttgagat gttttcagca cgcagtgctt
    72901 ttaatgctgc ggcaacgtcc agtacttgtt tcagttcatc tggcgtctgt tcccacgtta
    72961 acaaaaaatc tttctcgtga aggtgagagg tcagcgaatt gatatcctta atcagctcat
    73021 taacagtttt catcataaag tcccggtagt ggaatgacag gcaaagtggt ttatttcact
    73081 gtgacttgcc aaagtgattg gtgttttcac agctatacct gggactaaca ataagtgtgc
    73141 cagaattatc cgcgcggctg caaaagacta atttgacgga taatgtgtgg ggttgatcac
    73201 aaattgatgc cagcttctgg ttgtttgtaa aatacgaaaa cgtttggctt tttttatcgc
    73261 gttatttatt tgctgctaag tatcagaaat aagataaagg ggcaggctat ctctcttcag
    73321 tttgataaac tcaccgccgt aattctcata ttgatataga aatgtgatat ctcttccata
    73381 atttcattag aaaatgataa aaatcttata tttttatcaa agccaatttc gcctgagtta
    73441 atatcatcat gatctgtgaa ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg
    73501 aaggtaaagc tatggagctt gctactacgc agtcagtatt gatgcaaatt caaccgacaa
    73561 ttcagcgttt tgccagaatg cttgccagcg ttttgcagct tgaggttgag atcgttgatg
    73621 aaaacttgtg tcgcgtcgcc ggaacgggcg cgtatgggaa gtttcttggt cgccagttga
    73681 gcggcaactc acgcctgctc cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac
    73741 agtctcgctt cgatcccctt tgcgaaggtt gcgatagtaa agaaaattgc cgcgaaaaag
    73801 catttctggg tacgcctgtc attttacagg atcgttgtgt tggggtgata agtttgattg
    73861 ccgttaccca cgagcaacaa gagcatatca gtgataattt acgcgaattt tctgattacg
    73921 ttcgccatat atccaccatt tttgtttcga aacttctgga ggatcagggg ccaggagata
    73981 acatcagtaa aatatttgcg accatgatcg ataatatgga tcagggcgta ttagttgtag
    74041 atgatgaaaa tcgggttcag tttgttaatc agactgcctt aaaaacactt ggtgttgtgc
    74101 aaaataatat tattgggaaa cctatccgtt tcaga
//
LOCUS       DAZFZC010000011        73213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFZC010000011 DAZFZC010000000
VERSION     DAZFZC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.011"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(119..1066)
                     /gene="dusC"
                     /locus_tag="ACHKXP_000864"
     CDS             complement(119..1066)
                     /gene="dusC"
                     /locus_tag="ACHKXP_000864"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO7645931.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1305..1703
                     /gene="yohJ"
                     /locus_tag="ACHKXP_000865"
     CDS             1305..1703
                     /gene="yohJ"
                     /locus_tag="ACHKXP_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO7645932.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1700..2395
                     /gene="yohK"
                     /locus_tag="ACHKXP_000866"
     CDS             1700..2395
                     /gene="yohK"
                     /locus_tag="ACHKXP_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO7645933.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2525..3409
                     /gene="cdd"
                     /locus_tag="ACHKXP_000867"
     CDS             2525..3409
                     /gene="cdd"
                     /locus_tag="ACHKXP_000867"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO7645934.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3559..4278
                     /gene="sanA"
                     /locus_tag="ACHKXP_000868"
     CDS             3559..4278
                     /gene="sanA"
                     /locus_tag="ACHKXP_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO7645935.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4281..4520
                     /gene="yeiS"
                     /locus_tag="ACHKXP_000869"
     CDS             4281..4520
                     /gene="yeiS"
                     /locus_tag="ACHKXP_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO7645936.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIIYR"
     gene            4714..5952
                     /gene="preT"
                     /locus_tag="ACHKXP_000870"
     CDS             4714..5952
                     /gene="preT"
                     /locus_tag="ACHKXP_000870"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO7645937.1"
                     /translation="MPQQNYLDELTPAFTSLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            5946..7181
                     /gene="preA"
                     /locus_tag="ACHKXP_000871"
     CDS             5946..7181
                     /gene="preA"
                     /locus_tag="ACHKXP_000871"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO7645938.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7424..8434)
                     /gene="mglC"
                     /locus_tag="ACHKXP_000872"
     CDS             complement(7424..8434)
                     /gene="mglC"
                     /locus_tag="ACHKXP_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO7645939.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8450..9970)
                     /gene="mglA"
                     /locus_tag="ACHKXP_000873"
     CDS             complement(8450..9970)
                     /gene="mglA"
                     /locus_tag="ACHKXP_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO7645940.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLARLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGVIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(10031..11029)
                     /gene="mglB"
                     /locus_tag="ACHKXP_000874"
     CDS             complement(10031..11029)
                     /gene="mglB"
                     /locus_tag="ACHKXP_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO7645941.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11309..12349)
                     /gene="galS"
                     /locus_tag="ACHKXP_000875"
     CDS             complement(11309..12349)
                     /gene="galS"
                     /locus_tag="ACHKXP_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO7645942.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(12491..13648)
                     /gene="yeiB"
                     /locus_tag="ACHKXP_000876"
     CDS             complement(12491..13648)
                     /gene="yeiB"
                     /locus_tag="ACHKXP_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO7645943.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     RDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(13665..14333)
                     /gene="folE"
                     /locus_tag="ACHKXP_000877"
     CDS             complement(13665..14333)
                     /gene="folE"
                     /locus_tag="ACHKXP_000877"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO7645944.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14591..15427
                     /gene="yeiG"
                     /locus_tag="ACHKXP_000878"
     CDS             14591..15427
                     /gene="yeiG"
                     /locus_tag="ACHKXP_000878"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7645945.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15459..17440)
                     /gene="cirA"
                     /locus_tag="ACHKXP_000879"
                     /pseudo
     CDS             complement(15459..17440)
                     /gene="cirA"
                     /locus_tag="ACHKXP_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(17733..19202)
                     /gene="lysP"
                     /locus_tag="ACHKXP_000880"
     CDS             complement(17733..19202)
                     /gene="lysP"
                     /locus_tag="ACHKXP_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO7645946.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19407..20288)
                     /gene="yieE"
                     /locus_tag="ACHKXP_000881"
     CDS             complement(19407..20288)
                     /gene="yieE"
                     /locus_tag="ACHKXP_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO7645947.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20387..21436
                     /gene="yeiH"
                     /locus_tag="ACHKXP_000882"
     CDS             20387..21436
                     /gene="yeiH"
                     /locus_tag="ACHKXP_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO7645948.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21510..22367
                     /gene="nfo"
                     /locus_tag="ACHKXP_000883"
     CDS             21510..22367
                     /gene="nfo"
                     /locus_tag="ACHKXP_000883"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO7645949.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGMTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22370..23458
                     /gene="yeiI"
                     /locus_tag="ACHKXP_000884"
     CDS             22370..23458
                     /gene="yeiI"
                     /locus_tag="ACHKXP_000884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7645950.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNTAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23565..24815)
                     /gene="nupX"
                     /locus_tag="ACHKXP_000885"
     CDS             complement(23565..24815)
                     /gene="nupX"
                     /locus_tag="ACHKXP_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7645951.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24915..25856)
                     /gene="rihB"
                     /locus_tag="ACHKXP_000886"
     CDS             complement(24915..25856)
                     /gene="rihB"
                     /locus_tag="ACHKXP_000886"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO7645952.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25986..26684
                     /gene="yeiL"
                     /locus_tag="ACHKXP_000887"
     CDS             25986..26684
                     /gene="yeiL"
                     /locus_tag="ACHKXP_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO7645953.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26755..28005)
                     /locus_tag="ACHKXP_000888"
     CDS             complement(26755..28005)
                     /locus_tag="ACHKXP_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7645954.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(28099..29037)
                     /gene="psuG"
                     /locus_tag="ACHKXP_000889"
     CDS             complement(28099..29037)
                     /gene="psuG"
                     /locus_tag="ACHKXP_000889"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO7645955.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEIEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(29025..29966)
                     /gene="psuK"
                     /locus_tag="ACHKXP_000890"
     CDS             complement(29025..29966)
                     /gene="psuK"
                     /locus_tag="ACHKXP_000890"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO7645956.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEETLAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLTSCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(30390..32081)
                     /gene="fruA"
                     /locus_tag="ACHKXP_000891"
     CDS             complement(30390..32081)
                     /gene="fruA"
                     /locus_tag="ACHKXP_000891"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO7645957.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKLYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(32098..33036)
                     /gene="fruK"
                     /locus_tag="ACHKXP_000892"
     CDS             complement(32098..33036)
                     /gene="fruK"
                     /locus_tag="ACHKXP_000892"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO7645958.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(33036..34166)
                     /gene="fruB"
                     /locus_tag="ACHKXP_000893"
     CDS             complement(33036..34166)
                     /gene="fruB"
                     /locus_tag="ACHKXP_000893"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO7645959.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34538..35716
                     /gene="setB"
                     /locus_tag="ACHKXP_000894"
     CDS             34538..35716
                     /gene="setB"
                     /locus_tag="ACHKXP_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO7645960.1"
                     /translation="MTPPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTDE
                     VHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWNR
                     NYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGPP
                     LAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDTL
                     LLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFAK
                     RLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLMP
                     GQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKDV
                     "
     gene            complement(35713..35967)
                     /gene="yeiW"
                     /locus_tag="ACHKXP_000895"
     CDS             complement(35713..35967)
                     /gene="yeiW"
                     /locus_tag="ACHKXP_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7645961.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36122..36694
                     /gene="yeiP"
                     /locus_tag="ACHKXP_000896"
     CDS             36122..36694
                     /gene="yeiP"
                     /locus_tag="ACHKXP_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO7645962.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36917..38383
                     /gene="yeiQ"
                     /locus_tag="ACHKXP_000897"
     CDS             36917..38383
                     /gene="yeiQ"
                     /locus_tag="ACHKXP_000897"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO7645963.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38501..39487
                     /gene="yeiR"
                     /locus_tag="ACHKXP_000898"
     CDS             38501..39487
                     /gene="yeiR"
                     /locus_tag="ACHKXP_000898"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO7645964.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRTTPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39526..40239
                     /gene="lpxT"
                     /locus_tag="ACHKXP_000899"
     CDS             39526..40239
                     /gene="lpxT"
                     /locus_tag="ACHKXP_000899"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO7645965.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40651..41217
                     /gene="mepS"
                     /locus_tag="ACHKXP_000900"
     CDS             40651..41217
                     /gene="mepS"
                     /locus_tag="ACHKXP_000900"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO7645966.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41398..42954
                     /gene="pdeN"
                     /locus_tag="ACHKXP_000901"
     CDS             41398..42954
                     /gene="pdeN"
                     /locus_tag="ACHKXP_000901"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7645967.1"
                     /translation="MFIRAPNFERKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            43036..44850
                     /gene="yejA"
                     /locus_tag="ACHKXP_000902"
     CDS             43036..44850
                     /gene="yejA"
                     /locus_tag="ACHKXP_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO7645968.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            44851..45945
                     /gene="yejB"
                     /locus_tag="ACHKXP_000903"
     CDS             44851..45945
                     /gene="yejB"
                     /locus_tag="ACHKXP_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO7645969.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45945..46970
                     /gene="yejE"
                     /locus_tag="ACHKXP_000904"
     CDS             45945..46970
                     /gene="yejE"
                     /locus_tag="ACHKXP_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7645970.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46972..48561
                     /gene="yejF"
                     /locus_tag="ACHKXP_000905"
     CDS             46972..48561
                     /gene="yejF"
                     /locus_tag="ACHKXP_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO7645971.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIILRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(48565..48909)
                     /gene="yejG"
                     /locus_tag="ACHKXP_000906"
     CDS             complement(48565..48909)
                     /gene="yejG"
                     /locus_tag="ACHKXP_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO7645972.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49242..50432)
                     /gene="bcr"
                     /locus_tag="ACHKXP_000907"
     CDS             complement(49242..50432)
                     /gene="bcr"
                     /locus_tag="ACHKXP_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO7645973.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(50460..51155)
                     /gene="rsuA"
                     /locus_tag="ACHKXP_000908"
     CDS             complement(50460..51155)
                     /gene="rsuA"
                     /locus_tag="ACHKXP_000908"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO7645974.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51304..53064
                     /gene="radD"
                     /locus_tag="ACHKXP_000909"
     CDS             51304..53064
                     /gene="radD"
                     /locus_tag="ACHKXP_000909"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO7645975.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53189..53473
                     /gene="rplY"
                     /locus_tag="ACHKXP_000910"
     CDS             53189..53473
                     /gene="rplY"
                     /locus_tag="ACHKXP_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO7645976.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53612..54619)
                     /gene="yejK"
                     /locus_tag="ACHKXP_000911"
     CDS             complement(53612..54619)
                     /gene="yejK"
                     /locus_tag="ACHKXP_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO7645977.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAILLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54801..55028
                     /gene="yejL"
                     /locus_tag="ACHKXP_000912"
     CDS             54801..55028
                     /gene="yejL"
                     /locus_tag="ACHKXP_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO7645978.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            55048..56808
                     /gene="yejM"
                     /locus_tag="ACHKXP_000913"
     CDS             55048..56808
                     /gene="yejM"
                     /locus_tag="ACHKXP_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO7645979.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56883..56959
                     /locus_tag="ACHKXP_000914"
     tRNA            56883..56959
                     /locus_tag="ACHKXP_000914"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56917..56919,aa:Pro,seq:ggg)
     gene            complement(57062..59653)
                     /gene="yejO"
                     /locus_tag="ACHKXP_000915"
     CDS             complement(57062..59653)
                     /gene="yejO"
                     /locus_tag="ACHKXP_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFO7645980.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSNNTEINGGYQYIEMNGAAEYSVLNDGYQI
                     VQMGGAANQTTINNGVLQVYGAANDPTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FAFAVDQKAGGAIKATTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTVTGVDKKAGGKLIVSTNALEVSGPNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDKHATMQSLGKDTGTKVQANAVYDLGRSYQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLIFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            59973..60620
                     /gene="narP"
                     /locus_tag="ACHKXP_000916"
     CDS             59973..60620
                     /gene="narP"
                     /locus_tag="ACHKXP_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO7645981.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(60831..61883)
                     /gene="ccmH"
                     /locus_tag="ACHKXP_000917"
     CDS             complement(60831..61883)
                     /gene="ccmH"
                     /locus_tag="ACHKXP_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO7645982.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQTPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(61880..62437)
                     /gene="dsbE"
                     /locus_tag="ACHKXP_000918"
     CDS             complement(61880..62437)
                     /gene="dsbE"
                     /locus_tag="ACHKXP_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO7645983.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(62434..64377)
                     /gene="ccmF"
                     /locus_tag="ACHKXP_000919"
     CDS             complement(62434..64377)
                     /gene="ccmF"
                     /locus_tag="ACHKXP_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO7645984.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAVLRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSIMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(64374..64853)
                     /gene="ccmE"
                     /locus_tag="ACHKXP_000920"
     CDS             complement(64374..64853)
                     /gene="ccmE"
                     /locus_tag="ACHKXP_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO7645985.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(64850..65059)
                     /gene="ccmD"
                     /locus_tag="ACHKXP_000921"
     CDS             complement(64850..65059)
                     /gene="ccmD"
                     /locus_tag="ACHKXP_000921"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO7645986.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(65056..65793)
                     /gene="ccmC"
                     /locus_tag="ACHKXP_000922"
     CDS             complement(65056..65793)
                     /gene="ccmC"
                     /locus_tag="ACHKXP_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO7645987.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(65835..66497)
                     /gene="ccmB"
                     /locus_tag="ACHKXP_000923"
     CDS             complement(65835..66497)
                     /gene="ccmB"
                     /locus_tag="ACHKXP_000923"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO7645988.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(66494..67117)
                     /gene="ccmA"
                     /locus_tag="ACHKXP_000924"
     CDS             complement(66494..67117)
                     /gene="ccmA"
                     /locus_tag="ACHKXP_000924"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO7645989.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(67130..67732)
                     /gene="napC"
                     /locus_tag="ACHKXP_000925"
     CDS             complement(67130..67732)
                     /gene="napC"
                     /locus_tag="ACHKXP_000925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO7645990.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(67742..68191)
                     /gene="napB"
                     /locus_tag="ACHKXP_000926"
     CDS             complement(67742..68191)
                     /gene="napB"
                     /locus_tag="ACHKXP_000926"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO7645991.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(68188..69051)
                     /gene="napH"
                     /locus_tag="ACHKXP_000927"
     CDS             complement(68188..69051)
                     /gene="napH"
                     /locus_tag="ACHKXP_000927"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO7645992.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(69038..69733)
                     /gene="napG"
                     /locus_tag="ACHKXP_000928"
     CDS             complement(69038..69733)
                     /gene="napG"
                     /locus_tag="ACHKXP_000928"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO7645993.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(69740..72226)
                     /gene="napA"
                     /locus_tag="ACHKXP_000929"
     CDS             complement(69740..72226)
                     /gene="napA"
                     /locus_tag="ACHKXP_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO7645994.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(72223..72486)
                     /gene="napD"
                     /locus_tag="ACHKXP_000930"
     CDS             complement(72223..72486)
                     /gene="napD"
                     /locus_tag="ACHKXP_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO7645995.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(72476..72970)
                     /gene="napF"
                     /locus_tag="ACHKXP_000931"
     CDS             complement(72476..72970)
                     /gene="napF"
                     /locus_tag="ACHKXP_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO7645996.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            73079..>73213
                     /gene="yojO"
                     /locus_tag="ACHKXP_000932"
     CDS             73079..>73213
                     /gene="yojO"
                     /locus_tag="ACHKXP_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO7645997.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKYIYIAINSPEI
                     F"
BASE COUNT        17899 a        19615 c        18028 g        17671 t
ORIGIN      
        1 atccatgtgg gctgccccgg ccttcaggga acgcctcggc aattttgacg ccaccaaaca
       61 accgtgcggc ctattgataa agagctaaca cattgtcaaa aaacatcact atggtttttt
      121 agagtttctc gatatcaatt gcctgaatag cccttgcaat atcaggggaa ttattcaaca
      181 cccgaacatg ctgaaataat tccgttgctt catcgtattc tttacgcaaa taactcaacc
      241 actgtttaat ccgcgcaacg tgatataacc cggtatcgcc ctgcttttcc agacgggtat
      301 atttttgcag caaagcaacc acctccggcc acggcattcg cggttcgtta tattttacca
      361 cccggctcag gttaggaata ttgagcgccc cgcgaccaat catcactgcg tcgcagccgc
      421 tgatcgccat gcattgttgc gcgctctgcc agtcccagat ttcaccgttg gcaatcaccg
      481 gaatattcag ccgctggcga atatcgccaa tcgcctgcca gtcaatatgc tccgcgcggt
      541 aaccctgctc tttcgtccgc ccatgcacca ccagctccgt agcgccagcc tgttgaaccg
      601 catcggcgat ttcaaatttc ttctcaccgc tgtcccagcc cagacgcact ttcacgctga
      661 cgggcaaatg cgccggtaca gcttcacgca tcgcttttgc accctggtag atgagttcag
      721 gatctttgag taacgtcgcc ccgccgccgc taccgttaac cgttttcgac gggcagccgc
      781 aattgagatc cacgccccag gaacctaact ccaccgcacg ggcggcgttc tctgccagcc
      841 attgtgggaa ctgacctaac aactgcacgc gcaccagcgt accagatggt gtccggctgg
      901 cgttttgtag ctcagggcaa atgcgatgaa agacttttac cggcagcagt tgatccacca
      961 cgcggacaaa ctcggtgatg cacagatcgt agtcgttaac ttcggtcagc aattcacgca
     1021 ccagagagtc aagcactccc tccatcggtg ccagtaacac acgcatatca tcacccgcaa
     1081 aaaaatgagg cgctatgtta gcgcctatgg tcagcggatt aaaggtctgc aaagagggat
     1141 tgctgcaaac tgcaatccct ctctatgctt gatgtatgcc tgagttcgct gcgcattgcg
     1201 taatgccggg gagcgaaaaa ataagcgctg atttcttaat gtgatcggta gcacgtttta
     1261 cgaattaatt gtatgatgaa tccatctcat ctggggtgtt gattatgagt aagacactga
     1321 acattatctg gcaatattta cgcgctttcg tcctgattta tgcctgcctg tatgcaggca
     1381 ttttcattgc ttccctgcta ccggtaacca ttccgggcag catcatcggg atgctgatcc
     1441 tgtttgtcct gctggccttg caaattcttc cggcaaaatg ggtcaatccg gggtgctacg
     1501 tactgattcg ctatatggcg ctattgtttg tgccgattgg cgtaggcgtc atgcaatatt
     1561 ttgatttgct ccgcgcacag tttggcccgg tagtggtttc ctgtgcagtc agtacgctgg
     1621 tggtttttct ggtggtgagc tggagttcgc aactggtaca cggtgaacgt aaagtcgtag
     1681 gtcagaaagg atcagaagaa tgatggcgaa tatctggtgg tcattaccgc tgacattgat
     1741 tgtctttttt gccgcccgca aactggcggc acggtataag tttcccttgc ttaacccgtt
     1801 gctggtagca atggtggtga tcattccttt tttaatgctg actggcatct cttacgacag
     1861 ctactttaaa ggtagcgaag tgctaaacga tctgctgcaa ccggcggtcg tcgcgctggc
     1921 ctatccttta tatgagcagc tacaccagat ccgcgcgcgc tggaaatcga tcatcaccat
     1981 ctgttttatc ggcagcgtgg ttgcgatggt gacggggact tccgtggcat tgttgatggg
     2041 cgcttcaccg gaaatcgccg cgtcaatcct gccgaaatca gtcaccacgc ctattgcaat
     2101 ggcggttggc ggcagtattg gcggtattcc ggcaatcagc gcagtttgcg tgattttcgt
     2161 cggcatcctc ggcgcggtat ttggccatac attgcttaat gcgatgcgta ttcgtaccaa
     2221 agctgcgcgc ggtctggcaa tggggactgc ctcgcacgcc ctcggtacgg cgcgctgcgc
     2281 cgagctggat tatcaggaag gtgcatttag ttcgctagcg ctggtgttat gcgggataat
     2341 tacttcgctg atcgcaccgt tccttttccc gattattctg gcagtaatgg gctaaaattt
     2401 gcgatgcgtc gcgcattttt gatgtatgtt tcacgcgttg cataattaat gagattcaga
     2461 tcacatataa agccacaacg ggttcgtaaa ctgttatccc attacatgat tatgaggcaa
     2521 cgccatgcat ccacgttttc aaaccgcttt tgcccaactt gcggataact tgcaatctgc
     2581 actggaacct attctggcag acaagtactt ccccgctttg ttgaccgggg agcaagtctc
     2641 atcgctgaag agcgcaacgg ggctggacga agacgcgctg gcattcgcac tacttccgct
     2701 ggcggcggcc tgtgcgcgta cgccattgtc gaattttaat gttggcgcaa ttgcgcgcgg
     2761 tgtgagcgga acctggtatt tcggtgccaa tatggaattt attggtgcga caatgcagca
     2821 aaccgttcat gccgaacaaa gcgcgatcag ccacgcctgg ttgagtggtg aaaaagcgct
     2881 tgcagccatc accgttaact acacgccttg tggtcactgc cgtcagttta tgaatgaact
     2941 gaacagcggt ctggatctgc gtattcatct gccgggccgc gaggcacacg cgctgcgtga
     3001 ctatctgcca gatgcctttg ggccgaaaga tctggagatt aaaacgctgc tgatggacga
     3061 acaggatcac ggctatgcgc tgacgggtga tgcgctttct caggcagcga ttgcggcggc
     3121 aaaccgttcg cacatgcctt acagtaagtc gccaagcggt gtcgcgctgg aatgtaaaga
     3181 cggtcgtatt ttcagtggca gctacgctga aaacgccgca ttcaacccga ctctgccacc
     3241 gttgcaggga gcgttaattc tgttgaatct caagggttat gattacccgg atatccagcg
     3301 cgcggttctg gcagaaaaag ccgatgcgcc gttgattcag tgggatgcca cctccgcaac
     3361 gctgaaagct ctcggctgtc acagtatcga ccgagtgctt ctcgcttaag cctggtgccg
     3421 gatgcggcgt gaacgccttg tccggcttgc cagccctctc ctggtgtcga aattcccggc
     3481 aaacagtttg ccgtttcttg cgcaaaacca gcgggtaaag tagcctgatg gaaattttcc
     3541 ttagatcgag tctcctgcat gttaaagcgc gtgttcctca gcctgttagt cctgatcggc
     3601 ttgctgctgt tgactgtgct cggcctcgat cgctggatga gctggaaaac cgcgccttat
     3661 atctacgacg aattgcagga tctcccctac cgccaggtcg gtgtggtgct cggaacagca
     3721 aaatattatc gtactggcgt aattaatcag tattatcgct accgcattca aggagcgatt
     3781 aatgcctata acagcggtaa ggtaaattat ctattactga gcggcgataa cgcattgcaa
     3841 agttataatg agccgatgac catgcgcaaa gatttaatcg ctgctggtgt cgacccatca
     3901 gatattgttc tcgattacgc aggctttcgt acgctggatt ccatcgtgcg tacacgcaaa
     3961 gttttcgata ctaatgattt cattattatc acccaacgtt tccactgtga gcgagcatta
     4021 tttattgcgc tgcatatggg gattcaggct cagtgttatg ccgtaccgtc accgaaagat
     4081 atgctgtcag tacgtattcg tgaatttgcc gcccgtttcg gtgcgctggc tgacctttat
     4141 atttttaaac gtgaaccgcg ttttttaggg ccgctggtcc ctattccggc tatgcaccag
     4201 gtaccggaag atgcgcaggg gtatcccgcc gtcacacccg aacagttact tgaattacaa
     4261 aagaaacaag gaaagtagtt atggatgtac agcagttttt tgtcgttgcc gtttttttcc
     4321 ttatcccgat attttgtttc cgcgaagcat ggaaaggctg gcgcgcaggc gcgattgata
     4381 aacgggttaa aaatgcaccg gaaccggtgt atgtctggcg agcaaaaaat cccggactct
     4441 ttttcgctta tatggtggca tatatcggct tcggaatttt atctatcggc atgattgttt
     4501 atcttattat ctatcgttaa tattcccctc tccagttaat tattgagaat aattattact
     4561 tcacctgata agctgcggat atcacattcc taaccgcagc tatttgtgaa tcttttcaca
     4621 gtttaaattc ccccgcacgc ttagccttaa tatcagtaca ttattattta ctaaacgctc
     4681 gccttaatta cctatagcat taaggaagat cacatgccgc aacaaaatta tctggatgaa
     4741 ctcactccgg cttttacgtc tttactggcg attaaagaag cctctcgctg tttattatgt
     4801 cacgacgctc cctgtagtca ggcttgcccg gcacagaccg atccggggaa atttattcgc
     4861 tcaatctact ttcgtaattt taaaggcgct gccgagacaa ttcgcgaaaa taatgccctc
     4921 ggtgccgttt gtgccagagt gtgcccgacg gagaaattat gccaaagcgg ttgtacccgt
     4981 gccggtgttg atgctcccat tgatatcggc cgcttacagc gttttgttac tgattttgaa
     5041 caacaaaccg gaatggaaat ttatcagccc ggtactaaaa cgctcggcaa agtcgcgatt
     5101 attggcgcag gtcctgccgg attgcaggcc agtgtgacac tgacaaacca gggttatgac
     5161 gtcacgattt atgagaaaga agcgcacccc ggtggctggt tgcgtaacgg tattccgcaa
     5221 ttccggttac cgcaatcagt gctggatgca gagatcgccc gtattgaaaa aatgggcgtg
     5281 accattaagt gcaacaacga agtcggtaac acactcaccc ttgagcagct gaaagcagaa
     5341 aaccgcgcgg tactggtcac cgtggggtta tcaagcggtt ccgggctacc gctgtttgag
     5401 catagtgacg ttgagattgc cgtcgacttc ttgcaacgtg cacgacaggc gcaaggcgat
     5461 atcagcattc cacaaagcgc attaattatc ggcggcggtg atgtcgcgat ggacgtagcc
     5521 agcacgctga aagttctcgg ctgtcaggcg gtaacttgcg tagcgcgtga agagttagat
     5581 gagtttccgg caagcgaaaa agagtttacc agcgcccggg agctgggcgt ttcgatcatc
     5641 gatggattca cgccagtagc cgtggaaggc aataaagtca cgtttaagca tgtacggtta
     5701 tcgggcgaac tgacgatggc ggcagataaa attattctcg ccgtcggtca gcatgccaga
     5761 ctagatgcct ttgcggagtt agagccgcag cgtaacacca tcaaaacaca aaattaccag
     5821 acccgcgacc cgcaagtctt tgctgctggc gatattgttg agggtgacaa aaccgtggtc
     5881 tatgccgtga aaaccgggaa agaagccgcc gaggcgattc atcactattt agagggagct
     5941 tgctcatgtt aacgaaagat ctttcgatta ctttttgcgg cgtgaagttt cccaacccgt
     6001 tctgcctctc ttcttcgccg gtaggcaact gctatgagat gtgtgccaaa gcctacgaca
     6061 caggttgggg cggtgtggtg tttaaaacga tcggcttttt tatcgccaac gaagtctcgc
     6121 cgcgttttga tcatctggtg aaagaagata ccggttttat cggcttcaaa aatatggagc
     6181 agattgctga acatccgttg gaagagaatc tggccgccct gcgtcggctg aaggaagatt
     6241 acccggacaa agtattgatc gcttcgatca tgggggaaaa tgagcagcaa tgggaggagc
     6301 tggcgcgcct ggtgcaagaa gctggcgcgg atatgatcga gtgtaacttc tcctgtccgc
     6361 aaatgacttc tcatgcgatg ggtagcgatg tcgggcaaag cccggagctg gtagaaaaat
     6421 attgtcgggc agtgaaacgg ggttccacgc tgccaatgct ggcgaagatg acgccgaata
     6481 tcggtgatat gtgcgaagtg gcgctggcgg cgaagcgcgg cggcgcagat ggcattgcgg
     6541 cgattaacac cgttaaatcc atcaccaata tcgatcttaa tcagaaaatc ggtatgccga
     6601 tcgttaacgg aaaatcgagt atttccggat attccggtaa agcggtaaaa ccgatcgccc
     6661 tgcgcttcat tcagcaaatg cgcacccatc cagaactgcg cgatttccca atcagcggta
     6721 tcggcggcat tgaaacctgg gaggatgcgg ctgagttttt attgctcggc gcagcaacgt
     6781 tacaggtgac caccggcatc atgcagtacg ggtatcggat agtggaagat atggcaagcg
     6841 ggttgtcgca ttatctcgcc gatcagggat ttgattcgct gcaggagatg gtaggtctgg
     6901 cgaataacaa tattgtcccg gcggaagatt tagaccgcag ttatattgtc tatccccgta
     6961 tcaatcttga taaatgtgtt ggctgtggac gctgttatat ttcctgttac gacggcggtc
     7021 accaggcgat ggaatggagc gagaaaaccc gcacaccgca ttgtaatacc gagaaatgtg
     7081 tgggttgtct gctttgtggt cacgtctgcc cggtgggttg tattgagctc ggggaagtga
     7141 agtttaagaa aggcgagaaa gaacacccgg taacgttgta aaaccagaga cgcatccggc
     7201 atttggttcc tgtcgccgga tgcggcttga acgccttatc cggcctacaa aaccatacaa
     7261 atcagtatgt tgagcttatt gtaggccgga taaaacgcgc cagcgttgca tccggcattt
     7321 ggctcctgtt gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc acacagatca
     7381 tgatgttatg tgcgtttatg accgaatgcg gaccacattc acatcatttc ttacgcgcgt
     7441 atttcagtga atccagcgct acggcgaaga taataatcgc ccctttgatg atgtactgcc
     7501 agtatgggtt tacgccgata tacgtcaggc catagttgat gacggtaaaa ataattaccc
     7561 cggtcaccac gccaatcacc gtccccacac cgccgctgaa cgatacaccg cctaccacgc
     7621 acgccgcgat agcatccagc tcatacataa agccgaggtt gttggtggca gagccgatac
     7681 gtccggcttc taacatcccg ccaaaggcat agaacacgcc agacaacgcg tagatcatca
     7741 gcaggttcag gccgacgttg acaccagata cttttgccgc ttccgggtta ccgccaatgg
     7801 caaaaatgtt cttaccgaag cgggttttgt tccacaacac ccagacgaac gccaccgcaa
     7861 tcaacgcgta gaaggtgatg taagagagac ggaaactccc cagcgcgaca aagccctgag
     7921 caaaggtaga gaagccactg tcaaaaccag aaattggcga cgccccgaca aagtcgtaat
     7981 agagcgagtt gatgccatag acgatgatca tcgtgccgag cgtggtaatg aacggcgtca
     8041 cgttgagata agcgataatc agaccgttga tcaaaccgat caccgcacca atggcacaga
     8101 caatcagaat aaccagcgca atcggcatcg tcgccatttc cgggaacact ttgttggcgt
     8161 tatccatgga ctgcaataat gtcgcagcca ccactgccgc cagccctacc tgacgaccag
     8221 cagaaagatc ggtcccctgg gtgacaatta accctgccac accgagcgcg ataataatac
     8281 gcaccgatga ctgggtgaga atattactta agttcaacag acttaaaaat gttgggtcct
     8341 ggaaaataat aatcgccagc aaaactaaaa gaacgacgta aataccgccc tctttcaggt
     8401 aagtaagaaa acttttctta tttaacgcac tcatgggaag cccctaatct taaaggtgca
     8461 aagacgcaag acgcagaatt tcgttttgcg ttgttgtttt tgtatcgaca attccggaaa
     8521 cgagaccatt gctcatgacc agaatacggt ctgttatccc taacaactca ggcatttcag
     8581 aggagataat aataaccccc ttgcctttct tcgccagttc ggcaattaac tgataaattt
     8641 caaacttcgc cccgacatca ataccgcgcg tcggttcatc gagcattaat atttctggtt
     8701 gcgttagtag ccagcgacca ataatcacct tttgctgatt accaccggag agcgaaccaa
     8761 tttgcgtccg atgacccggc gtttttaccc gcatcgaatc aatcacccac tgggtatcgc
     8821 ttttcatccg cgagttatcc agtaaaccaa ctttattttt gtagttgcga atattggaaa
     8881 ttaacgagtt aaaaccaata tccagatagg cataaattcc cgttgagcgg cgctcctcag
     8941 ttaccagtgc aaatccgtgg tttatggctt cgttggcatt atggttattg atctgtttgc
     9001 cgtgcaacgt aatggtgcca gccgatttct cgcgaatacc aaataacgtc tcaacaatat
     9061 cggtacgttt cgcccccacc agcccggcaa taccgaggat ctccccttta tgcagatcaa
     9121 acgagacatc gcgaatcgac ggctggcgca gtgacgttag gttacgcacc tcgaggatga
     9181 cttcgcccgg cttgttttct ttgtcaggaa aacgctgatt aagagaacgc ccaaccatca
     9241 tggcgatgat cttgtccatc gtcagtcttg ccagcggttc ggtggcgatc cactgaccgt
     9301 cgcgcaatac ggtaacttca tcacataact ggaagatttc ttccatttta tgcgagatat
     9361 aaacaatacc gcagccgcgc tcttttaatt tacgaataat agtgaacaga tgattgacct
     9421 ctttttcggt taacgaagaa gtcggttcat ccataatcac aattttcgcg ttataggaaa
     9481 acgctttggc gatttcgatc atctgcattt gcgaaacgga taatgtgccg acgcgcgcac
     9541 gcggatcgat atcaatatcc agttcatcaa aaatcgcttt ggtttcgcgg tacattttgt
     9601 cctgatcgac aaacatgcct ttggtgggat atcgccccag ccacatgtta tccatcaccg
     9661 aacgttgtaa taccaggttt aactcctggt gtaccatcga aataccattt tccagtgcct
     9721 ctttggcgga atggaaatcg atctctttac cctggaataa aatggtgccg gagtcttttt
     9781 gataaatacc aaacaggcat tttaataatg tcgattttcc tgcaccgttt tcccccatta
     9841 atgcatggat agaatgtggc cggactttta aattaacgtt atcaagtgcc ttaacaccag
     9901 gaaaggactt gttgataccg ctcatttcca acaagtattc cccggaagac ggagtcgttg
     9961 agctgaccat ataattttac cttgttggcc atacaataag ggcgcagtaa acgactgcgc
    10021 ccaatcagtc ttatttcttg ctgaattcag ccaggttgtc tttatctacg ccaacataag
    10081 gtacgcggac cactttgttg tcgattttcc agttggtgcc atcagccgca cctttgccat
    10141 cggccaggtt tttcgccaga tcaaaggtcg ctttcgcctg gttgttagca tcgttcagta
    10201 ctgtgcccgc cagtgcaccg gatttcacca gcgccagcgc ttctggcagc gcatcgacgc
    10261 caaacaccgg aatgctggac ttgttgtgtg ctttcagtgc ttctaccgcg cccattgcca
    10321 tcgcatcgtt gttggcgata accacttcga ttttgttggc gttcgggcca gacagccagg
    10381 cgtccatctt atctttcgcc tgagcggtat cccacatagc ggtatctaac tgtaactgtt
    10441 cagttttaat gcctttgtcg ttcagctctt taatcacgta agtggtacgt gcttctgcat
    10501 ccggatggcc cggttcacct ttcagcaata cgaattgaat ctgaccgtct ttgttcagat
    10561 cccaaccctg attcgccgcc cagtgtttag caatcaaatc gccctgaata atgccggact
    10621 ctttggaatc agtgccaacg tagtaggctt tgtcgtagct atccagcgcc ttacgagacg
    10681 gttctttgtt gaagaaaacc accggcacgt tttgcccacg cgctttctca atcaccgtac
    10741 ccgcagctgc cgggtcaacc aggttgattg ccagtgcctt cacccctttc gccagcaata
    10801 cgtcgatctg atcgttctgc ttggactggt cattctgaga atcattcatc agcagctgaa
    10861 catctggcgc ggctttcgca tcttgctcaa tagccttgcg cactacagac ataaagttat
    10921 cgtcgtactt atagattgtt acaccaatgc gagtatcagc agcgtgtgca gcggcaccga
    10981 ataacatgct ggccatcaca gcagacaggg ttaacacctt cttattcatg gtatctccgg
    11041 tttttcttat gcagggtagt gcttgagata aatgctcggc ggggcagtag agttaatgaa
    11101 gtgttactga acgccgaagc tcacttttta aaattcgttc ttccatgccc ggtaacgctc
    11161 cagaaaacgg ctttaattgt tgttatgacg ctattacctc ggtgaaagtg attattcact
    11221 gttacatacg ggttacaacg ttaaaacggt gcaatcatag ccatcacatt gttaagatac
    11281 tgtgaaatca ctcacagatt gaaagcggtt acatcgcctg atttgttgag ttagtgatcg
    11341 ccgccgcatt ctggcgcgtt gcgacagaat ggcgacgcac taacgtcggc ataaaacagt
    11401 ggctggcacg aggatcaata ttgcctgctg ccccctgcaa ggccagttcg gtggctaatt
    11461 tagccattga agcaatggga taacgcacgg tcgttaattg cgggtcggtg taacgggcga
    11521 tgggaatatc atcgaaaccg atgattgaga gatgtaatgg aatcgcaatg ccattatctt
    11581 ttaatgctgt cagtgcgcca gcggccatat tgtcgttata agcaaataca gcggtaagtt
    11641 gtagattgcg ccccagcagt tcaaccatcg ccgcctcacc gcccggcatg tccggcgtac
    11701 cagtgccaat ccagctttcc ggcggaataa tatcctgctc tttcaacgca ctcatccagc
    11761 ctgctttacg catggcgtca tcttcaatgc cgtggctgga agaaagataa ccaatacgtt
    11821 gatgaccgtt attcagcaac atgcgcgtcg ccattcgggc accgctgaga ttatccaggc
    11881 aaacgcaacg atgggcgtac cccggcacaa cgcggttgat taacaccata ccgggaatgt
    11941 tatccataaa ttgcgccagt tcatcgtcac tcaatgcttt tgagtgaaca atcaacgcat
    12001 tacaacgctg gcgaattaac acctcaatgg cgtgacgctc tttttccgct tcatgatagc
    12061 tattgccgat tagcacgtat ttctgatgct gctgagcgac cagatccacc gcttttacca
    12121 gcgcgccgaa aaacgcatca gaaacgtcca tcaccaccac gccaatggtg tcgctaacct
    12181 gagttgccag cgcctgggca ttggcgtttg gccgataatc cagctcactc acggctttca
    12241 ttactgcttc acgcgtgtcg gcactgacca gcgtgctgtt attgagcacc cgggaaaccg
    12301 ttgccacaga gacgccagcc tgacgcgcta catcacgaat ggtgatcata ttcactacct
    12361 gttatgagat tgcagtaaat gactgcttgc tggcggctat tttgtcagca ctgaatacag
    12421 gacttcgtga atcgagtcac agcaatggaa acggttacag ccgttttgtt aacgaatgtg
    12481 atccagatcg ttatcttgat gttttagata ttgccggtcc ggcagcacgc aaagttaact
    12541 gacgccagag ccattccacc ggcccctggc ggaagtaacg cagccagata acagagaaga
    12601 ggatattcgc cagccatacc ggaataacaa acgccagcag ctccaggcgg tcaaaatgca
    12661 taaacaaacc gaggtggtaa aaaagcgtgg tacaaatcag cgtttgcaat agatagttgg
    12721 tcagcgccat ccgtccgacg caggcgatcg caagcaccag cttaaagcgg ctcaattgcg
    12781 gccagaagcc ataaaacagc gacgcatagc caatcgcctg aaacggcgca ctcagttccc
    12841 gcggcatttg aagtaagaag gcacaccagc gatacgccca gtccagctgc cattgcaggg
    12901 cgatggcagg aaggttaatg gtcaccccaa tcgccaccag cacaaaacca gtacgacgat
    12961 agtgacgtaa gctgaactgc cctttcagcc agccgctgcg catcagcgcc gcaccaatga
    13021 gcatcatccc tgccagttgc cagccatact gtgcgcccag tgccagtaaa ctgttgccaa
    13081 caccatcggc acgattactg atcgcttcaa cgccgccgtg aagcttccag tatttttcat
    13141 acaaaatagc cgatgcatcc ggcgtccagg cgcggctggt ctggctgtcg gaaatcagcc
    13201 ccaataacag caaaacgcca agccccacca gataaagcat gacgcctgta ttaaacaggc
    13261 tttttaccga tggcgcatcg cgcaccagcc gccagcagat taagcccacc agcccgtaag
    13321 ccagcaaaat atcgccgtcc cagaacaata aaccgtgaat aaagcccaac aagactaaca
    13381 gcgttaaccg cgactggatc cagcgtctgc cacggggcag caacatttgc aggcccgcac
    13441 caaacagtag cgcaaaaagc gtgaggaatt tcacctggcc gatgagatca agaaatgccc
    13501 aagtccatgc atcccgcggc gtaatagcac cgtaccaggc gggattgaga taagccgcct
    13561 ttggtagccc aaaggcgctg atgtttaata gcaggatccc cagaatggcg acgccgcgaa
    13621 caaaatcgag cgtgacgttg cgctccatgg ttcctgcctt ttaatcagtt gtgatgacgc
    13681 acagcgcgca gaaactcgtg gcgcgtattc tgactggatt tgaacaatcc accaagagag
    13741 gtcgttgtcg tggcactggt tgcatcgcgg atgccacgcg ccttcacgca gtaatgcacc
    13801 gcgtcgatcg agacagccac gttattggtg cccagcagcg tttgtagcgc aataagaatt
    13861 tgctgcgtca gacgttcctg cacctgcgga cgctgggcaa agaactgcac aatgcggtta
    13921 atttttgaca gaccgatcac cgaatctttc gggatatagg ccaccgtcgc tttgccatcg
    13981 atggtaacaa aatggtgttc acaggtgctg gtcagagtga tatcgcgcac ggtgaccatt
    14041 tcatcgacct tcattttgtt ttcaatgagg gtgattttcg ggaaattggc gtaatccaga
    14101 ccggagaaaa tttcatcgac atacatttta gcgatgcgat gcggcgtttc catcaaactg
    14161 tcatcagcca ggtcgagatt cagcagctgc atgatttcgg tcatatgacc agcaataagg
    14221 cttttgcgcg tttcgttatc catttcatgc acgggcgggc gcagcggtgt ttccagtcct
    14281 cgcgcaacta acgcttcatg aaccagggcc gcttctttac tgagtgatgg catttatgat
    14341 ttctcctgca ggtgtgacgc ctccgccctg cgtgggggca aagttattaa gctgatttac
    14401 agcctgatta ttgtgcgtga ggcggcgcac ataatccagt attcacagcg ataattattg
    14461 taattgccgc tgcctttcat cagcagatgt taaaacatcg ttatgcaaat acggaagtga
    14521 aagttactca cagcacattg aataaacggt atgatgaaga aattgcaaac aacacaacaa
    14581 ggagccacgc atggaaatgc tcgaagagca ccgctgtttt gaaggctggc agcaacgctg
    14641 gcgacacgac tccagtacct taaactgccc gatgacgttc agtatctttc tccctccacc
    14701 tcgtgatcac actccgccac cagtgctgta ctggctttcc ggattaacct gcaatgacga
    14761 gaacttcacc accaaggcgg gtgcccagcg ggtagcggcg gaactgggga ttgtactggt
    14821 gatgccagac accagcccgc gcggcgaaaa ggttgccaac gacgatggct acgatttagg
    14881 ccagggcgca ggcttttatc ttaatgccac gcaaccgccg tgggcgacgc attaccggat
    14941 gtatgattat ctgcgcgatg aattaccggc gctggttcag tcgcaattta atgtcagcga
    15001 ccgctgcgcc attagcggtc actcaatggg tggtcacggt gcgctgatta tggcgctgaa
    15061 aaatccgggt aaatacacca gcgtttcggc ctttgcgcca attgtgaatc cgtgcagcgt
    15121 cccgtgggga atcaaagcgt ttagcagcta tttaggtgag gacaaaaatg catggctgga
    15181 atgggacagt tgcgcactga tgtatgccag taacgcgcag gatgcgatcc cgacgcttat
    15241 cgatcagggc gataatgatc agtttcttgc cgaccagttg caacctgcgg tactggcaga
    15301 agccgcgcgc cagaaagcgt ggccgatgac gctgcgtatt cagccgggat atgatcacag
    15361 ttactacttc atcgcctctt ttatagagga tcacctgcgc ttccatgcgc agtatttact
    15421 gaagtgaaag tccgcccggt tcgccgggca tcttctcatc agaagcgata atccactgcc
    15481 ataaagtaac gacgtccgtc ttcgttatag ctgtagtcgt cacgactgag atccttgtcg
    15541 ccaaggttca gcacgcctgc gcgcagtttg acgtctttag tcacctgcca ggccgcgccg
    15601 gtattccaga tggtataacc gcccggtgtt ttagccgtcg cgctgtcggc gcgtttctgc
    15661 ccggtatagt gcccagaaac atagaatgac cagtcttcca gcgccagcgg tttccagtcc
    15721 agcgtaccgt tagcagtatg gaacggcaga tcggatagcg gtttgttttc gccgttgctg
    15781 acatcacgac catcgttgta ggtgtagttg atcgacagtt tccattcatc gttgaacgga
    15841 attttcagtt cggtttccac gccctgaata cgagctttgt taacgttgta gtagctaaat
    15901 accggtatgc gccgtccgtt agcgcccgtc tcaagttttg gtagcccggt gcagcgttga
    15961 cgtcagacgt acggctgatg ctgatacgat ctttcacatc gttacggaaa acggtaacgc
    16021 tggattcaac cccttccagc cagccttctt cacccatgta gtaaagcccc agctcccaac
    16081 tttcgctggt ttctggtttc agatccgggc tacccacaat cttacatgcg ccacggcagg
    16141 aattgctcgt ccagtcaggg ctaagttgca acagagaagg tgctttaaat gccgtcgccc
    16201 agcccccttt caccgttacg gtgtcggtgg cgttataaac caggtaggca cgcggactcc
    16261 agtgttcacc gtaggtttca tgatcgtcca tacgtacgcc ggtcgtcagc gccagcggct
    16321 cgaagatccg ccattcatct tccacaaaca gcgcgtactg gctggcagac gttttggagc
    16381 tggttccccc ggtcaggttc accgcatcgc taagtttgtc gtgacgccat tcaccgccaa
    16441 ccgtgagaaa ctgattaatc gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc
    16501 tttcggaagt tatcgggctg ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt
    16561 atttcagttc gctggtgccg taatcccaac gcccattatg gctgacggag tagttctggc
    16621 gttccaggcg gtttttgtcc agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc
    16681 cggcagtaaa atcgtgattt tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc
    16741 tggagaatcc ttcaatacgc ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt
    16801 catccttttc acgttttgcc aggctgccgt aagctttcat tcccagcacg ccgtcaatta
    16861 atggtccgct ggtaaagaac tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa
    16921 cggtggtatc gacggtaacg gtgcccgacc atttctgacc gatttttttg gtgatgatat
    16981 tcactacacc gccgagcgca tcggaaccgt acagcgacga catcgggcca cggaccactt
    17041 caatacgttc gatggaatcg accgggatcc agttcagatc gaaatcattg tggcggaaga
    17101 cggcattgcg ggagttaacg cgtttaccgt cgacaagaat cagggtgtag ctgctgtcca
    17161 gaccacgaat actaacgccc ttacggttat ccccttcgtt cgtcagttgt acgccaggca
    17221 cttctttgag gacatccttc agattctgta ccggttttcg ctgcaggtct tcctgggtaa
    17281 tgacgctgat actggcgggt gcatctttaa ggttttgttc aacggaagat gcagtgacaa
    17341 ccatcgtttc gccatcatca tcgaccgcta acacaggcca tgcacaagaa atagcggaca
    17401 aacacagccc gacccgtacg aaagggttca acctaaacat tccatatctc catgaggtaa
    17461 ctacgaaaat aaaatgggtt atcgctcaca tcttcttcac gtccccttgc gtacggcagc
    17521 atcgcggtgg acttatttgt atgcaggtga tcatcccaga aagccggacc agcctctgat
    17581 ttgtcagcct tttttgattg cggcgtaacg ataatgcaaa cgataacaat tatcaatcca
    17641 aatgttaaat tttatatccg catgatttgt cagggaaatg gaattgaaaa agccctctcg
    17701 gttgagaggg cttagcaagg aagggaggaa acttatttct tatcgttctg cgggaacttc
    17761 atttcgctgt agcgtacgaa gtgagttcct ttaatcagct tgtagccgaa ccaaataatc
    17821 aggaacagcg ggataccaat atacgttgcc gctacgccgc cccagtcaat agtatctttc
    17881 aggaacgctt cgtagttctg gcccaaagtg ataatcagac acagaatgaa tgcgaagatc
    17941 ggccccaatg ggaagaaacc tgaacggtac ggcagatcgt taatgtcgtg tccctgcaat
    18001 acgtaaccgc gacggaagcg atagtggcta atggcaatcc ccagccaggc gataaaaccc
    18061 gtcatcccgg aggtattcag cagccacagg tataccgtct ggttgccaaa catggaggtc
    18121 aggaagcaca gaccggcaat caccgtcgtc gcatacagcg cattacgcgg cacgccacca
    18181 cgcgacagtt tagcgaaaat gcgcggcgct ttaccgtcac acgccagggt gtacagcata
    18241 cgagtagacg catacatacc ggagttaccg gctgacagca ccgccgtcag aataactgcg
    18301 ttcatcaccg ccgccgcaga gagcagaccc gcgtgctgga acaccagggt gaacggacta
    18361 acgctgatgt ctttaacatc gttacgcagc aggctcggat cggtgtacgg aataatgagg
    18421 ctgataatca ggatcgcgaa cacatagaac aacaggattc gccagaacac ctgacgtacc
    18481 gcgcgtggaa tgtttttcgc cggatcttcg gactcgcctg cagcaatacc gatcagctcg
    18541 gttccctgga aagagaagcc gacaatcata gctacgccga tcatcgccgc aaaaccacca
    18601 gcaaacggcg cttcgccgat tgtccagttg ctccagcccg caggctgcgc gcctttgaag
    18661 ataccgataa tcatcagcac gccaacgatg ataaagacaa taactgtcgt gactttgatc
    18721 agtgagaacc agtattccgc ttcaccaaag ccacgaactg agatgtagtt cagcaggaag
    18781 ataacgccga ggaacaacgc actccagatc cagcccggtg tatccgggaa ccaccagctc
    18841 atgaccagct gagctgcaac caggtcaacg gcgatagtca ccgcccagtt gtaccagtag
    18901 ttccagccca gcgcgaagcc aaagccttct tcaacatagt tctgaccgta agtggcaaac
    18961 gaaccggaaa ccggcatata tgcagccagt tcaccgagac tggtcatcag gaagtaaacc
    19021 atcaggccaa tcaacatata cgagagcaat gccccgcccg gacctgcctg agaaatcgtt
    19081 gcgccagagg caacaaaaag acctgtaccg atggaaccgc caatggcaat catcgtcagg
    19141 tgacgcgcct ttaattcacg gcgtaagccc ggcgcttctg tggttttagt ttcggaaacc
    19201 atacaaaaat gctatccatc taaaaaatga ggcgcgattg tagcagacga ttggcaatcc
    19261 ttccggcaga aatacacggt tataagacac cttcatgatc gcccagggat tataagtaaa
    19321 gcagccaatc tcttttctgg cgaatgcatg ctgaaggcgc tgcgtcacga aatcgacaca
    19381 gcaccagcat gttcttgtac agcaacttaa cgcggtacat tcgcgggatc gcaatagtcc
    19441 agaaagcgcc gtagcgcgtt ggaaaggtgt ttttgccgat gatgtatacg ccacaacgta
    19501 cgcatcaggc gcggcagagg gaccgcaact tcacttaatg tgcctgcctg caattgatct
    19561 tcaatcaccc gtcgcgacag gcaactaatt cccaacccat gacgcaccgc atgtttgatt
    19621 gcctcggagt tacctaattc catcgccatc tcaaacttcg gtaaatgtga cagcaataga
    19681 taatcgacaa tctcccgcgt gccggaaccg cgttcacgca ggatccacgg cgcagcggcc
    19741 agctgctcta aggtgaccgg accacgggcc aacggcgaag tcggcgcggc gaaaaccacc
    19801 agctcgtctt ccagccacgg ttcagaaatg atttcagtgc tgtggcacgg tccttcaata
    19861 aagccaatat caacgcggaa atccagcacc gcttgcatca cgtcctggct gttcccaacg
    19921 ctaagttcaa tcggcaactg cggataatca tggcgataac gggcaataac tgcaggcaga
    19981 atgtagttac cgatggtact actggcatag atacgaatcg cgccgttgtc ttcgcgaaac
    20041 agttgttcga tttcaaccgc ctgttcaagc agtgccaacg cacgcggata gagcagccga
    20101 ccgtgttcat taactaccag tcttttcccc acgcgatcaa acagttgcac gccaagctgc
    20161 ccttccaggt cggtcaaggc tgcgctcact gctgattgcg acaacgccag catcaccgac
    20221 gcctgggtgg ttgatccact tttcaatact tctgcaaaaa cttccaactg ccggagggtg
    20281 atgtgcatag tcgcttacca cttataaaga ttaattataa atatataatc aattttattt
    20341 ttaaaccagt tagtcgtaac cttatgcccg gttaaaggag agggttatga cgaatatcac
    20401 cttacagaaa caacatcgta cactgtggca ttttattccg gggttagccc tgagtgcagt
    20461 tatcaccggg gtcgccctgt ggggtggttc cattcccgcg gttgcgggtg ccgggtttag
    20521 tgccctcacc ctcgcaatct tgttggggat ggttttaggc aacaccatct atccgcacat
    20581 ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa tatttattac gtctgggtat
    20641 cattctttat ggcttccgtc tgacgttctc gcaaattgcc gatgtcggta tcagtgggat
    20701 catcattgac gtgttgacgc tgtccagtac cttcctgctg gcttgcttcc tggggcagaa
    20761 agtgtttggt ctggataagc acaccagctg gttgatcggt gccggtagca gtatctgtgg
    20821 tgctgccgcg gtactggcga ctgagccggt agtgaaagcg gaagccagta aagtaaccgt
    20881 ggctgttgca accgttgtta tcttcgggac cgtcgcgatt ttcctctacc cggcgatata
    20941 tccgctgatg tctcaatggt ttagtccgga aactttcggt atctacattg gttctactgt
    21001 gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc agcccggatg cggaaaacgc
    21061 agcagttatt tccaaaatgc tgcgcgtgat gatgctggct ccgttcctca tcctgctggc
    21121 ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag aaaagcaaaa tcactattcc
    21181 gtggtttgcc atcttgttca tcgtagttgc catctttaac tcgttccacc tgttaccgca
    21241 gagcgtggtg aacatgctgg taacgctgga taccttcctg ctggcaatgg cgatggcggc
    21301 gctgggtctg accactcacg tcagcgcgct gaaaaaagct ggggcgaaac ctctgctgat
    21361 ggcactggta ctgtttgcct ggctgattgt tggtggtggt gcgattaact atgtgattca
    21421 aagcgtcatc gcataaacca ctacatcttg ctcctgttaa cccgctatca ttaccgtttt
    21481 cctccagcgg gtttaacagg agtcctcgca tgaaatacat tggagcgcac gttagtgctg
    21541 ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat cgacgcaacc gcgtttgcct
    21601 tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg
    21661 aattcaaagc cgcctgtgaa aaatatcact acacatcggc gcaaattctt ccccacgaca
    21721 gttatctgat taacctcgga catccggtca ctgaagctct ggaaaaatcg cgcgatgcct
    21781 ttatagatga aatgcagcgt tgcgaacagc tggggctttc tttgctcaac ttccaccctg
    21841 gcagccatct gatgcagatt tcagaagagg attgccttgc gcgtattgcc gaatccatca
    21901 acattgcgct ggataaaact caaggtatga cagcggtgat agaaaacacc gccggtcagg
    21961 gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat tatcgacggc gtggaagata
    22021 aatcccgcgt cggcgtctgc attgatacct gccatgcttt cgctgccggg tatgatttgc
    22081 gtactccagc cgaatgcgag aaaacattcg cggattttgc ccgtactgtc ggctttaagt
    22141 atctgcgcgg gatgcacctt aacgatgcga aaagcacctt tggcagccgc gttgaccgcc
    22201 atcatagcct cggtgaaggc aatatcggtc atgatgcgtt ccgctggatc atgcaggacg
    22261 accgtttcga cggcattccg ctgatcctcg aaaccatcaa cccggatatc tgggcagaag
    22321 agatcgcctg gctgaaagcg caacaaactg aaaaagcggt agcctgaaga tgaataaccg
    22381 ggaaaaggag atccttgcaa ttttacggcg taacccgctg attcagcaga acgaaattgc
    22441 ggacatgctg caaatcagcc gttcgcgcgt tgcggcgcat attatggatt taatgcgcaa
    22501 aggccggatt aaaggcaaag gttacattct caccgagcag gaatactgcg tagtggtggg
    22561 gacaatcaat atggatattc gcgggatggc ggatatccgt tacccgcaat cggcttctca
    22621 tcccggtaca attcattgct cagcgggcgg cgtgggacgc aacatcgccc acaatctggc
    22681 gctgttaggc cgtgacgtcc atttgctttc agtgattggc gatgactttt atggcgaaat
    22741 gctcctggaa gaaacgcgcc gtgccggcgt gaatgtctcc ggctgcgttc gtttgcatgg
    22801 tcaaagcaca tcgacgtatc tggcaattgc caatcgagac gatcaaaccg tgctggcgat
    22861 taacgatacc catctgctgg aacagttgac accgcaacta ctgaacgggt cgcgcgattt
    22921 acttcgtcat gcgggcgtgg tactggctga ttgcaacctg acagccgagg cgctggaatg
    22981 ggtctttacc ctcgctgatg aaatcccggt gtttgtcgat accgtttcag aattcaaagc
    23041 gggcaaaatc aaacactggc tggcgcatat tcacaccctg aaacccactt taccggagct
    23101 ggaaatttta tggggacagg cgatcaccag cgatgctgac cgtaataccg cagtgaatgc
    23161 attgcatcag caaggtgttc agcaactgtt tgtttatttg cccgatgagt cagtttattg
    23221 cagcgaaaag gatggagaac aatttttgct gaccgcgcca gcgcatacga cagtagacag
    23281 ttttggtgct gacgatggtt ttatggcggg cctagtatat agctttcttg aaggatacag
    23341 tttccgcgac agcgcccgtt ttgcggtagc ctgcgcggca atttcgcgcg ccagcggcag
    23401 cttaaacaac cctaccctgt ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca
    23461 atgttgccgg atgcgacgct taacgcgtct tatccggcct acaaatcagg caatttcagc
    23521 aagtaaatgt aggcctgata agcttgcgca tcaggcaatg acactcaagc taaaccaata
    23581 aagaacccgg caatggtcgc actcatcaag ttggaaagcg tcgccgccgc cagcgcccgt
    23641 aaaccaagct gggcgatttc cggcgcacgg tgtggcgcaa ccgcagaaaa cgcccccacc
    23701 accaccccga tagaaccaaa gttagcgaaa ccgcacaacg cgaaggaaat aatcgccaca
    23761 gttttagcat cgagagtgcc agccgtttgc agatagggtg agaaattgag ataagcgaca
    23821 aattcattta ttgccagttt ctgtccaatc aaactcccgg caagattcgc atcactccag
    23881 tccacaccca tcacccacgc cagtggagcc agcaggtaac ctaaaatgga ctccagcgag
    23941 gcatgttcaa aaccaaacca gccaccaacg ccgccgataa taccgttaat caacgcaatt
    24001 attgcaacaa atgccatcac cactgttgcc acacctgcgg cgattttcag cccggtcatt
    24061 gcccctgtcg cagcggcttc aataatgctt tttggcggtg tttcggtgaa agagaggtta
    24121 ttaaaggaaa cctgcgaaga ttccgttgcc gggcttaaca ggcgggcaaa caagatcccg
    24181 ccagggatcg ccattaatga tgccgccagc agatattcca caggcacgcc cagtgcggcg
    24241 taaccaatca ttgtcgaacc agcaatcgag gccatgccac tacaaatcgc tgtaaataat
    24301 tcattgcgat tcagacgatc gataaagggt ttgacgattg ccggaatttc gttttgcccg
    24361 aggaaaatgg tggtgaccgc gacgaatgac tcgatcttgc tgatatttaa tgctttctgg
    24421 aagataccgc cgagaattcg aattaaaatc cccatcacac cgatgtagta gagaatactc
    24481 accagcgcgg tgacgaagat aattgccggt aacaccctga aaccaaagat aaatcctgca
    24541 ccatcaaata aggtatccat tttcggtccg accagagaac cgaagataaa tgcgctaccc
    24601 gcgtcgctgt acgccatcac tttatgcacg ccaaaagcga ctttttcagc gacccaacgc
    24661 cctggcggta accaaagcat aatgccgcca atcacgacct gtaacactaa cgccgcgcca
    24721 acggtacgca ggctgatctt cttcttgttt actgacagta aaaacgcaat cgtcagcaat
    24781 accaccattc ccagaacact tctcatgaca tccatagtga ttatctcttc atgtcatatt
    24841 gggcgacggc tttctgccca tcagaaatag ccaggcctgc atcttgcgat accggcccgg
    24901 cttgttatca acacttaatg ggttttgatg tagccgcgca cgcactcttc gactaatccc
    24961 cagaaccagt ctgtatcaat agtgatgccg actttggtat tggcgggctt gccaagaacg
    25021 cccagctcgt cgcagacggt acgcccatag caagggccac tgttgacgtc cacttcgacg
    25081 tacatctcct gggttttaat gccatcaggg ttaatcagat aaccgatgca ggtggcgtcg
    25141 tgcaccgggc cgccagcaag gccgtagttt tcgaactgcg ttttgagagt gaagttcatg
    25201 atgtcgctga acagctctcc ggcggggccg cctgcccttt ccatccgagc aatcacgtcc
    25261 ggggtgcaaa cggtctggtt ggtgagatcg aggcccatca tcactaatgg aacgccggag
    25321 gtgaacacta cgcgtgcggc ttccgggtcg gcaaagatgt tgaactcggc agatggcgtg
    25381 aagttgcctg taccgtaagc gccgcccatt agcacaattt cacggatttt gggcaggatc
    25441 gcgggttgca tacgcattgc caccgcgata tttgaaagcg gaccaaccgg caccagagtg
    25501 atatcgccat cgcttgccat cagggtatca atgatatatt tcaccgcatg agtgctttct
    25561 gcctggcggg tcagcggctc gaataccggg ccatccagtc cggtttcacc gtgaatatta
    25621 tcggcaacga tttgttgacg cataatgggc tgcggcatcc ccgcataaac cggaacatta
    25681 atctccagtt tctggcaaac attcaggcca ttaattaatg ttttatcaag cgtctgatta
    25741 cccgctacaa tagtgatgcc taataaatct attgccggat gtttcgccgc catcattata
    25801 gcaatagcat catcatgacc cggatcacaa tccagaataa tttttctctt ttccattgtt
    25861 tatttcctct gtttccagtt gcgttatttt ttctacagca aagaaaattc gaccgggccg
    25921 atgattgaat cttaacaaca gcgtacgtat gctaaatatg agaaatctca tagcggataa
    25981 acatcgtgaa agaaatccac aataatgatc ttaagcagca attgatgagt gaatccgcgt
    26041 ttaaggattg ctttttaacg gatgtttcag ccgatacgcg gctgtttcat tttttagcgc
    26101 gtgactacat tgtgcaggaa gggcaacagc cgtcctggct gttttacctg acgcgaggcc
    26161 gcgccaggct ttacgccacg ctagctaatg gtcgcgtgtc gctgatcgat ttctttgccg
    26221 ccccctgttt tattggcgag attgagttaa tcgataaaga ccatgaaccg cgtgcggtgc
    26281 aggctattga agagtgttgg tgccttgcgc tccctatgaa acattaccgt ccgctgttat
    26341 taaacgacac gctattttta cgaaaactct gcgtcacctt aagtcataaa aattatcgta
    26401 atattgtttc tttaactcag aatcaatcat ttccgttagt gaatcgcctg gcagcattta
    26461 tattactctc gcaggaaggt gatctttatc acgaaaagca tacgcaagcg gcagagtatt
    26521 taggcgtttc ttatcgacat cttttatatg ttctcgcgca gttcattcac gacggtttat
    26581 taattaaaag caagaaaggg tatctcatta aaaacagaaa gcagttgtca ggactggcgc
    26641 tggagatgga cccggagaat aaattctccg ggatgatgca gtaaaaatta ttttcaatag
    26701 cgcgattgcc ggatgcaacg cttaatacgt tttatccggt ctacaaatcg agcattacgc
    26761 cagaccaata aagaacccgg caatagtcgc actcatcagg ttggaaagcg ttgctgctgc
    26821 cagcgcccgt aaaccaagct gggcgatttc cggcgcgcgt tttggcgaaa tagccgaaaa
    26881 tgcgccaacg acaacaccga tagaaccaaa gttagcaaaa ccacaaagcg caaaggagat
    26941 aatcgcaatg gttttcactt ccagcgtgcc gcccgtttgc aggtatgggg agaaactcag
    27001 gtaagcgacg aattcgttaa tcgccagttt ctgcccaatc aggctacccg caagattggc
    27061 atcactccag tccacaccca tgatccacgc cagcggtgcc agcacatagc caaaaatact
    27121 ttccagagag gcattggcga aaccaaacca gccgccaatt ccgccgataa tgccgttgat
    27181 cagcgcaata attgcgacaa acgccattac caccgtcgcc acaccagcgg cgatttttag
    27241 cccggtcatc gcaccgctcg ccgccgcttc gataaagctt tttggcggcg tttcgctgaa
    27301 cgacagattt tcaaatgtga cctgcgaagg ctcggttgcc gggctaagaa tacgtgcaaa
    27361 caaaatcccg ccagggatcg ccatcagcga tgccgctaac aggtagtcaa ttggtacgcc
    27421 cattccggca taaccaatca tcatcgaacc agcaatggac gccatcccgc tacaaattgc
    27481 ggtaaacaac tcgttgcgat tcatgcgatc gataaacggt ttaacgatcg ccgggatctc
    27541 attttgcccg aggaaaatag tagtaaccgc aacaaaagat tcgattttgc tgatgttgag
    27601 ggctttctgg aaaatgctgc caaggatgcg aatcagcagc cccatcacgc caatgtagta
    27661 cagcagactg atgagcgcag taacgaaaat aatcgccgga agtacgcgaa aggcgaagat
    27721 aaaacccgca ccgtcaaaca ggacatccat tttcggccca accagcgaac caaaaatgaa
    27781 ggcgctaccg gcatcactgt aagacatcac tttatgaacg cctaatgccg cctgttctac
    27841 tgcccatttt cccggtggga agtagagcat gatgccacca atagcgattt gcagcagcag
    27901 tgcggctcca accgtgcgca aactgatgct ctttttattc actgacaaca gaaatgctat
    27961 tgccagtaac accaccatcc ccacaacact tctcattata tccataatga ttttcccttc
    28021 atgccggtaa acccggcgtc agcgccaggt tttggtatgc ttgatgagta cgggcgacgg
    28081 ctttctgccc gtcagaaatt aacccgcgag acgctgatat tctttggcaa tttcgctcgc
    28141 cagaatggcg ttgttgaaca ccagctggat gttggatttc aggctgtcac cgccggtcag
    28201 ttcagcaacg cgcgccagca ggaatggcgt actttcttta ccaatgactc cctgagcttc
    28261 agcttcagct accgcctgat cgatcgccgc attgatagtg tgttccggca tagcaaactg
    28321 ttccgggatc gggttcgcta ccacgaggcc accgttcaga ccgctttgcc atttcaccgc
    28381 cattgcacgg gcaatttcgc tggcgctgtc gagacgaatg ctgacgtcaa acgggctggt
    28441 gcggcagaaa aacgcaggca gcgctttagt ctgatagcca attaacggca caccgaaggt
    28501 ttctaaatac tcagtggtta atcctaaatc gagaatagat ttcgccccgg cacaaacaac
    28561 ggtgacatta gtatttgcca gttcttgcaa atcggcagaa atatcgaagg tatgttccgc
    28621 cccgcgatgc acaccaccaa ttcccccggt ggcaaatact ttaattccgg caagcgccgc
    28681 aataatcatc gttgaagcca cagtggttgc gccatttttt ccggcggcaa caacaaaagg
    28741 taaatcgcga cgactaactt tggtcacgtt atgcccttca cgacccagta attcaatttc
    28801 ttctttgctt aaccccactt tcatcacgcc gccaataatg gcgatcgtgg caggtacagc
    28861 gccctgttta cgaatagttt cttcaatttc aattgcggtc tgggcatttt gtgggaacgg
    28921 catcccgtga gaaataatgg tcgattccag cgccacaacc ggttttttgt tttttaaagc
    28981 gtcctgcact tccggggaaa tttgtaataa ttcaggggaa atttttaatt cagacattct
    29041 gcgttctcca ctaacgatat aacgttggca atcgataaat cggggttatt ggtgtattca
    29101 caggagagcg ccattgacga acatccctgt gcgaaacgaa cagattcggc aaacggcatt
    29161 ccgtctaccc aacacgaagt aagtcccgcc atcatggcat cgcccgctcc ggtaacatta
    29221 ataacattgg ttttgatcgg cgcagaccag ccactttcac cgctgatatc gctgtaataa
    29281 acgccgtcgc cgcccatgct caataccagt cggttcaggc catgttgatg gaaccaggca
    29341 gcaacttttg ccacatcttc acgccctgac agcgcaatcc cactcagggt ttccgcttca
    29401 aggcggtttg gcttgagagt gtggatctga tttagacggt cgcgcacttt gacacatttc
    29461 catgccgaaa ccggatcgac aaatacgggt acgttggcag cattatccag aatccatgcc
    29521 agtgtctctt cactgatatt acagtccgcg acaatgacct ttgccctctg aataaattca
    29581 cggtgctgtg cgagatattc agctgtaata gcgttgctaa tattcatgtc atttatagca
    29641 accagcattt caccggtatt atcgagtaat gataaatagc tcgacgtatt ttctcccggc
    29701 acaatcaggc atttatcgac ataaacgcca gattgattgg tttgcgttag cagcgattgg
    29761 ccataaaaat cactgcctac ggcgctcagt agccaggctt tattacccag caacgccagg
    29821 ttttgtgcaa tattgcgccc taccccacca ggcgtaaatt ttattttacc tggatttgaa
    29881 tccgcataat ttaatgattc atgtgaatat ccggcgacat caatattcgc cgagcctata
    29941 attacgacat aatccttttc gcgcatagca cgtcctctgg taaaaaatta ccaaccctgt
    30001 caatccacaa gaatatagat actgcaaaga cagtttaaac atgtgttcat atttaaacat
    30061 gtgctcatag tatgcatgtt ttacgtaaag taaattgccg ccagagaggc aaagcgggca
    30121 gatttgagca ggctcacaaa atgcgattaa atgccagaat tacaaaagcg aggaatttgt
    30181 gacgggatgc acagaacatg aacactaaaa aagggcagaa aagaatctgc ccttgaggaa
    30241 ataaccttag tgtttgatga acgtttcagg tttctactga aagataaaac catttaaatt
    30301 cataaaatta cattcacaaa ccggtcattc tctttgcgct catccaatga tacagggcag
    30361 taaaattctg ccctgtaaca caccttttat tacgcggctt tcgctactgc gtccacttcc
    30421 ggacgtttca ggaaggcata ggccaaaccc gccaccagcg taccggcaat aattgctacc
    30481 aggtaaccca gtaccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgc
    30541 ggtgccatca gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc cccacccacg
    30601 atacagcacg gcagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa
    30661 ataaagcaca gtcccagtac cagagcagct ttgccacctt cctgctgcgc tttgtcgaat
    30721 ttgcgacgcg ccaccattgt tgccagaccc attgccagcg gtggcaccat acctgccgcc
    30781 ataatcgccg ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac
    30841 gctgctttat ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc
    30901 agcagaaccg cattcgcagt ccccatggtc tgcagccagt gagtcaaccc ttcgagaatg
    30961 ccagcaaccg gtttaccgat caggtagatc atcgccagac cgaccaccag actggaaatt
    31021 agcggaatga tcaggatcgg tttcagcgcc tccatactct gtggcagttt cagttgcgta
    31081 ctgattaact tcgcaatgta accagccagg aagcccgcaa taataccgcc aatgaagcca
    31141 gaaccggtgc tgaccgccag cataccacca atcagacccg gcgtgagacc cggtcgatcg
    31201 gcaatggaga aggcaatgta acctgccagt accggcacca tcagcgcaaa ggctgaacca
    31261 ccaccaatct gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca
    31321 aaagcaaaag aaagcgcgat acacagacca cctgcaacga ccatcggcag catgtaagag
    31381 acacccgtca gcaagtgacg gtatgcgcct gcactctctt tcttaccttc agaggtcgcc
    31441 gtttgagctt tgcccgccgg ttcatacggc gttgcttcag caaccgcttt atccagttcc
    31501 tgcgcggttt tcttcagcgc cagaccggta gaggtacgat acatcggttt accagcaaat
    31561 ttcgccagat ccacttcgat atctgccgcc acaatcacca gatccgctgc ggctacttct
    31621 tcgggagtga ttgcattacc cgcaccaaca gaaccacggg tttcaacttt cacccaccag
    31681 ccacgttttt tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg tgctacgcca
    31741 gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc tggcggcaac cggtgctgtc
    31801 gcagcgaccg gcgcagtgta aagtttcgca tggcctttgg cttcactcag gaacagctca
    31861 gggtgcgcaa ctgcccggga aatatcgccc agccagacat ttttaccgtt cagcgcgctg
    31921 tcattcggga tggaatcacc gagaacaatc gccatttctg cgtcgttcgg attgtcgatg
    31981 atttccagtt ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt
    32041 gcctgaccga gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca
    32101 gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat
    32161 acccacattg ctttgactta ccgccagggc tgcaacagca gtcgccagac gcagtgtgtg
    32221 ttcactggat tcacgcatta gcaagccata aatcaggcca ccaaccatag aatcccctgc
    32281 gccaacggtg cttacgacat cgactgacgg tggtttggcg atccattcgc cggaggcatt
    32341 aacccaaagc gcgccttcgg cacccagtga aataacgaca tgcgcaatac cttgttcacg
    32401 cagcgcatgc gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat
    32461 ttccagctcg cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa
    32521 cgcttcacgg ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca
    32581 gtcggtgaac gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc
    32641 gaactgaccg agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgggggtgac
    32701 ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt
    32761 aattcgggtg cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag
    32821 ttgctgaaaa ccatcctgat tgtctttacc caggaagccg ccaacggtga catcaattcc
    32881 caggtctttt aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt
    32941 tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc
    33001 cggattaagg gtgatagtag caacacgtct gctcattatg cgccctcccc aagaccagca
    33061 gcgatagcgt cgccgattgc tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag
    33121 cgtaggcgat gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt
    33181 gccggtttgc cggtaccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg
    33241 gtattgacca gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac
    33301 tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt
    33361 gccgcatccg ctttcagcaa gcggtcagct ttattgtcga gcaataaatc agcgagacgc
    33421 ttaagaaccg cgatgggctg atcgtcattc atcgccacac tcaccagcat ggctgccgtt
    33481 tcgccgtcca catcaaaagc atttgccgca cggcttaccg caatcgcgct acgcagattg
    33541 ccttcggcgc tatcgctcag ccagataccc tgtccgagat tcagcggttg ttcattgatg
    33601 gctttggtga cgaaagtggc gtcaactgcc cccgcctctt tcagacgcgc agcgttcagc
    33661 gcctgaagag tcagcagatc gctggcgacg atatccagtg tcagcatttc gttgtcgagc
    33721 ttcagctgct cactctgctt ttcgcccatc agtaatgcgc gaagttcttc tgctgttgtt
    33781 gctgacttca gttgttcagc aacggaatca tcgctcagta cgtgggtcag ctggcgtagc
    33841 aggcccagat gttcatccga gctggcagca ataccgattg ccacgtacgc tacctgaccg
    33901 tcaccccagg tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga
    33961 tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt
    34021 tgctgttcgc gcgccagcat gccattgacg tagccttctg ctacattacc ggcctgcacc
    34081 agcgccgcag cgacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg
    34141 atgtcctgta cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag
    34201 cctctatgag aaaaaaagcg ccaacctggc ttagggttaa agacaagatc gcgctgaaac
    34261 gtttcaagaa agcataatac ttctgtttca gcacgcaagg aaagccgtaa agttatgagc
    34321 aaaagtttga tgtgctgcac aattttttgc catttttcct aattgctgat gggaaaatcc
    34381 gttatcagca atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt
    34441 ttgactattc gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa
    34501 ctccgcgtct tcctcttcca gtgatcgacc agcatgcata actccccccg cagtctccag
    34561 cgcgaaatcg tttgacctga cctcgacggc gtttttaatc gttgcctttc tcaccggtat
    34621 tgcgggcgct ctgcaaaccc cgacactcag tatttttctt accgatgaag tacatgcccg
    34681 tccggcgatg gtgggattct tctttaccgg cagcgctgtc attgggattc tggtaagtca
    34741 gtttctcgcc gggcgctctg ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg
    34801 cctgttaggc gtgctggcct gcaccctttt tgcctggaat cgcaactact ttgttttgct
    34861 attcgttggc gtctttctta gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct
    34921 tgcccgtgaa catgccgaca aaaccggacg tgaggcggtg atgttcagct cttttttacg
    34981 cgctcaggtt tcactggcat gggtcattgg cccaccgctg gcttatgcct tagcgatggg
    35041 tttcagcttt acggtaatgt atctgagcgc agcggtagcg tttattgttt gcggtgtgat
    35101 ggtgtggctg tttttaccgt cgatgcaaaa agagcttccg ctggcgaccg gcacgatcga
    35161 agcgccgcgc cgtaaccgtc gcgatacgct gctgctgttt gtcatttgta cattgatgtg
    35221 gggctcgaac agcctgtaca tcatcaacat gccgctattt attatcaacg aactgcatct
    35281 tcccgagaaa ctggccggtg tgatgatggg gaccgccgcc gggctggaaa tcccgacgat
    35341 gttgattgcc ggatatttcg ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc
    35401 cgtgggtggc gtctgttttt acgcaggaat gctgatggcg cattcacctg tcattctgtt
    35461 gggcttgcag ctgctaaatg ctatttttat tggcattctg ggcggcatcg ggatgctcta
    35521 ttttcaggat ctgatgcccg gtcaggcggg ttcagccacc acgctctata ccaacacttc
    35581 gcgcgtgggc tggatcatcg caggatcagt ggcgggcatc gtcgccgaga tctggaatta
    35641 tcacgctgtg ttctggtttg cgatggtgat gattatcgcc actctgtttt gcttactgcg
    35701 gattaaagat gtttaaggcg cggtcagcat ttccagatcg ataagccagg tcatggcctg
    35761 ctgacgcgag ttaccgcaca tttcagcgct ggcttgtaag cctgcacaga ctttcggtcg
    35821 cagtggcgag gtgaaaattt tacagcgttg ctgttcatca agctgaatgc agggcgtatt
    35881 ggcgggcttg ccatctggca taccgggaat agggctggaa attgaagggg cagtgcaaca
    35941 cgccccgcaa cccggacggc attccatacg ttgttctctt aaggttaacc accggattga
    36001 tgagcgcgca cagtaccacc ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa
    36061 aggcccgtcg cgagtacttt gtcgcgatat ttttgacatt ttcgactaca ggaatttttc
    36121 gatgccaaga gcgaacgaaa ttaaaaaagg tatggtactg aattacaacg gcaaactgct
    36181 gctggttaag gatattgata ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa
    36241 aatgcgtttt tctgatgtcc gtaccgggct gaaagtagaa gagcgtttca aaggtgatga
    36301 tatcgttgac accgtgacgc tgacccgccg ttacgttgac ttctcctatg tcgatggcaa
    36361 cgaatatgtc tttatggata aagaagacta taccccgtat accttcacca aagatcagat
    36421 tgaagaagag ttgctgttta tgccagaggg cggcatgccg gacatgcagg tgctgacctg
    36481 ggacggtcaa ctgctggcgc ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac
    36541 cgcaccaggc atcaaagggg catctgccag cgcccgtaac aaaccggcga cattgagcac
    36601 tggtctggtg attcaggtac cagaatactt aagcccgggc gaaaaaattc gtatccatat
    36661 cgaagaacgc cgttatatgg ggcgtgctga ctaacttcag ccgcatgcag aaaagggata
    36721 gctcaggctg tccctttttt aatttattat accaatcttc tattttgcgc ttcctgaaca
    36781 ccttacgccc tcaattttca ctcgttgatt gatcgccctc acacttcatc gcattaacaa
    36841 tccagaccaa tttcaattgc tgtcatataa ctttacactg tcgttgttaa ttaatcgtta
    36901 ctaagacgtg actcctatga atacaatcgc ctccgttacg ctcccgcatc atgtacacgc
    36961 tccacgctat gatcgccagc agttgcaatc acgtatcgtt cattttggct ttggagcctt
    37021 tcaccgcgct catcaggcgt tactgaccga tcgtgtgctg aatgcccagg gcggcgactg
    37081 ggggatctgt gaaatcagct tgttcagcgg tgatcaactg atgagccagc tccgcgcaca
    37141 gaaccattta tataccgtgc tggagaaagg tgcggacggc aatcaggtga taattgtcgg
    37201 tgccgttcac gaatgcctta atgcaaaact ggattcctta gcggcaatta ttgagaaatt
    37261 ttgcgagcca caggtggcaa ttgtttccct gacgattacc gaaaaaggct attgtattga
    37321 cccggccacc ggtgcactcg acaccagtaa tccgcggatt attcacgatc tacaaacccc
    37381 tgaagaacct cactccgcac cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg
    37441 cggccttaca ccgtttaccg tgctctcctg cgacaatatt cccgacaatg gtcatgtggt
    37501 gaaaaacgcg gtgctgggaa tggcagaaaa acgttcgcca gaactcgccg ggtggataaa
    37561 agagcacgtc agttttccgg gaaccatggt cgaccgcatt gttccggctg caaccgacga
    37621 atcactggtg gaaatcagcc agcatctggg ggtgaatgat ccctgcgcga ttagctgcga
    37681 accgtttatc cagtgggtgg tggaagataa cttcgtcgct gggcgtcctg cctgggaagt
    37741 cgcaggtgta caaatggtga atgatgtcct gccatgggaa gagatgaaac tgcggatgct
    37801 taatggcagc cactcttttc tcgcttatct gggttacctc tcaggattcg cccatatcag
    37861 tgattgcatg caggatcgcg catttcgcca tgccgccaga acattaatgc tggatgagca
    37921 agcgccgaca ctgcaaatta aagatgtcga tttaacacaa tatgcggata agttaattgc
    37981 acgttttgct aatccggcgc tgaaacataa gacctggcaa atcgcgatgg atggcagcca
    38041 gaaattaccg caacgcatgc tggcaggtat tcgcatacat caggggcgcg aaacggactg
    38101 gtcgttgctg gcattaggcg ttgcaggctg gatgcgttac gtcagcggcg ttgatgatgc
    38161 cggaaatgcc attgatgttc gcgatccgct tagcgataaa attcgcgaac ttgttgcggg
    38221 cagcagcagt gaacaacgcg taaccgccct gctttccctg cgtgaagttt tcggtgatga
    38281 tctgccagat aacccgcatt ttgtgcaggc catcgaacaa gcctggcaac aaatcgtaca
    38341 attcggcgca catcaggcgc tattaaacac cctcaaaatt taacgatttc tgcggttaaa
    38401 gcggatgaag ctcaccttcg tccgctctcc ccttctcttt tctgcctttt ttagccagga
    38461 ttaacgctca gttaacttac cagagtattg cggagccgtt gtgaccagga ccaacctcat
    38521 caccggtttt ctcggcagcg ggaaaaccac gtcgattctt catctgttag cccataaaga
    38581 tcccaacgaa aaatgggcgg tactggttaa tgaatttggg gaagtcggaa ttgatggtgc
    38641 tttgctcgcc gatagcggcg cattgctgaa agagatcccc ggcggctgca tgtgctgcgt
    38701 taatggttta cccatgcagg tagggttgaa taccttactg cgtcagggaa aaccagaccg
    38761 cttgttgata gagccgaccg ggctgggcca tccgaaacag atcctcgatc tgttaaccgc
    38821 accagtctat gaaccgtgga tagatctgcg cgccaccttg tgcattctcg atccgcgctt
    38881 gctgctggac gaaaaaagcg ccagcaatga aaacttccgt gaccagctgg ctgccgcaga
    38941 catcattgtc gccaataaat ccgaccgtac gacgcccgaa agtgagcaag cgctacagcg
    39001 ttggtggcag caaaatggtg gcgatcgaca attaattcac agtgagcatg ggaaagttga
    39061 cggtcatctt ctggatttgc cgcgtcgcaa tttagccgag ttgcccgcca gcgccgcgca
    39121 ttctcatcag catgtcgtga aaaaagggtt agcagcgtta agcctgccag agcatcaacg
    39181 ctggcgtcgc agtctgaaca gcgggcaagg atatcaggcc tgcggctgga tattcgacgc
    39241 tgatacggta ttcgacacca ttggcattct ggaatgggcg cgacttgcac cggtggaacg
    39301 cgtcaaaggc gtgctgcgta ttcccgaagg gctggtgcga atcaaccgtc agggcgatga
    39361 cctgcacatt gaaacgcaaa acgttgcgcc accggacagc cgtattgagc tgatttccag
    39421 cagcgaagct gactggaatg ccttgcagag cgcgctgttg aagcttcgtt tagcgactac
    39481 cgcgtaaggt tgcctgcgtt tttcagtaag ataattagag aaaatatgat taaaaatttg
    39541 ccgcaaatag tgttgttgaa tattgtcggc ctcgcgctgt ttctttcctg gtatatcccc
    39601 gttaatcatg gattctggtt gccgattgat gcggatattt tttatttctt taatcagaaa
    39661 ctggtcgaaa gtaaggcctt tttgtggctg gttgcattga ccaacaatcg cgccttcgac
    39721 ggttgttcac tgctggcgat gggtatgttg atgctgagtt tctggctgaa agaaaacgcc
    39781 cctggcagac gacgtatcgt gattattggt ctggtcatgc tattaactgc agtggtatta
    39841 aaccagctgg gtcaggcatt aattcctgta aaacgggcca gcccaacatt gacttttacc
    39901 gatattaacc gcgtcagcga actgctctct gttcccacga aagatgcctc acgagatagc
    39961 tttcccggcg atcacggcat gatgctgctt attttttcgg cattcatgtg gcgttatttc
    40021 ggcaaagttg caggccttat cgcccttatt atttttgtgg tttttgcatt tcccagagta
    40081 atgattggcg cacactggtt tactgacatc attgtcggtt cgatgaccgt gatattgatc
    40141 ggtttgccct gggtgttgct gacgccatta agtgatcgat taatcacctt ttttgacaaa
    40201 tcactaccag gaaaaaacaa acatttccaa aacaaataac tcacagtaat taacatcatc
    40261 agggttattt ttatagtgag gataatcctg atgatgcgca ccgtgctttc atctatcgaa
    40321 cgcaaaaatc attctctaag taaatgaatg gattgcatgc gtttcactca attgtacttt
    40381 aattgaccaa ccccgcttat taactttctg tatcactttt tcttataaaa aatcatgtaa
    40441 aaccgctcgc caagaccgca ccaatcgggt aatctcgaac tcgttttgcc tcggcggtag
    40501 attatcctca cagcatataa ttttgtgcgt tagtccacag atttggcctt aaggaattgt
    40561 ttcaacatgc ccaggtaatt agtctcgtgt cgcttggcat ttttttataa cgatatttgt
    40621 cgttaaggac ttcaagggaa aacaaacaac atggtcaaat ctcaaccgat tttgagatat
    40681 atcttgcgcg ggattcccgc gattgcagta gcggttctgc tttctgcatg tagtgcaaat
    40741 aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg gtagtgaaac atcatcactg
    40801 caagcttctc aggatgaatt tgaaaacctg gttcgtaatg tcgacgtaaa atcgcgaatt
    40861 atggatcagt atgctgactg gaaaggcgta cgttatcgtc tgggcggcag cactaaaaaa
    40921 ggtatcgatt gttctggttt cgtacagcgt acattccgtg agcaatttgg cttagaactt
    40981 ccgcgttcga cttacgaaca gcaggaaatg ggtaaatctg tttcccgcag taatttgcgt
    41041 acgggtgatt tagttctgtt ccgtgccggt tcaacgggac gccatgtcgg tatttatatc
    41101 ggcaacaatc agtttgtcca tgcttccacc agcagtggtg ttattatttc cagcatgaat
    41161 gaaccgtact ggaagaagcg ttacaacgaa gcacgccggg ttctcagccg cagctaataa
    41221 accgtttgga tgcaatccct tggctatcct gacgagttaa ctgaaagcac tgcttaggca
    41281 gtgctttttt gttttcattc atcagagaaa atgatgtttc cgcgtcttga tccaggctat
    41341 agtccggtca ttgttatctt ttaaatgttg tcgtaatttc aggaaattaa cggaatcatg
    41401 ttcatacgcg ctcccaattt tgaacgtaag ctcctgctta cctgcattgt tgcaggcgta
    41461 atgattgcga tactggtgag ttgccttcag tttttagtgg cctggcataa gcacgaagtc
    41521 aaatacgaca cactgattac cgacgtacaa aagtatctcg atacctattt tgccgacctg
    41581 aaatccacta ctgaccggct ccagccgctg accttagata cctgccagca agctaacccc
    41641 gaactgaccg cccgcgcagc gtttagcatg aatgtccgaa cgtttgtgct ggtgaaagat
    41701 aaaaaaacat tctgttcatc tgcgaccggt gagatggaca ttccactcaa tgaattgatt
    41761 ccggcgctcg acattaataa aaacgtcgat atggcgatct tacccggcac gccgatggtg
    41821 ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt tgctgaaaaa tagcggcgtc
    41881 tttgccgctc tgaatctcaa cctgacgcct tcactctttt atagttcacg gcaggaagat
    41941 tacgatggcg tcgccctcat tattggcaat actgcgctat ctaccttttc ttcacgtttg
    42001 atgaacgtta acgaattaac cgacatgcca gtccgtgaaa ctaaaattgc gggcattcct
    42061 ctgaccgttc ggctttatgc agatgactgg acatggaacg atgtgtggta cgcattttta
    42121 ctgggcggca tgagtggaac tgtcgttggc ctgctctgct attacctgat gagcgtacgt
    42181 atgcgccccg gcagagaaat catgaccgcc atcaagcgcg aacaatttta cgtggcgtat
    42241 caaccggtgg tggatacaca agctttgcga gtaacgggcc tggaagtact gctacgctgg
    42301 cggcatcctg tcgcgggaga aattcccccg gatgccttca ttaactttgc cgaatcgcaa
    42361 aagatgattg tgccgctgac tcagcacctg tttgagttaa ttgcccgcga tgccgcagaa
    42421 ttagaaaaag tgctgccggt aggcgtcaaa tttggtatta acattgcgcc ggaccatctg
    42481 cacagcgaaa gctttaaagc agatatccag aaactgctca cttccctgcc cgcacaccat
    42541 ttccagattg tgctggaaat taccgagcgc gatatgttga aagagcaaga agccacacaa
    42601 ctcttcgcct ggctgcactc ggtcggcgta gaaattgcta ttgatgactt cggcaccggg
    42661 cacagcgcgc ttatctatct tgagcgtttt acgctcgatt atctgaaaat tgaccgtgga
    42721 tttatcaacg ccatcggtac ggaaacgatc acttcccccg tacttgacgc ggtgctgacg
    42781 ctggcgaaac gcctcaatat gctgacggtt gctgaagggg tcgaaacgcc ggaacaggcg
    42841 cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct actggattag ccgcccgtta
    42901 ccgctggacg attttgttcg ctggctaaag aaaccgtata cgccgcagtg gtaaggtgtg
    42961 cttacgtccc ttattattca tagtgaaagc atgccggatt gcggctaatg atgagtaaaa
    43021 ggaaatccgt tgcagatgat tgtgcgcata ctgctgctgt ttatcgccct gttcaccttc
    43081 ggtgcgcagg cgcaggctat caaggaaagc tatgcctttg ccgtgctggg cgaaccccgg
    43141 tacgcattta atttcaacca ttttgattat gtgaaccccg ccgcgccaaa aggtgggcag
    43201 ataacgttgt cagccctcgg caccttcgat aatttcaacc gctatgcact gcgcggcaac
    43261 ccgggcgcac gcaccgagca gttgtacgac acgctattta cgacttccga tgacgaacca
    43321 ggcagttatt acccgctgat tgctgaaagt gcacgctatg ctgacgatta ttcctgggtg
    43381 gaggtcgcta ttaatccgcg cgcccgtttt catgatggtt cgcccattac tgcccgcgat
    43441 gtagagttta cttttcaaaa atttatgacc gaaggcgtgc cgcaatttcg tctggtctac
    43501 aaaggcacca ccgtcaaagc cattgcaccg ttaaccgtgc gcattgagtt agctaaaccc
    43561 ggcaaagaag atatgctgag tctgttttcg ctgccggtat ttccagaaaa gtactggaag
    43621 gatcacaaac ttagcgaccc gctcgccacg cctccgcttg ccagtggtcc gtaccgcgtt
    43681 acgtcctgga aaatggggca aaatattgtc tattcccgtg tgaaagatta ctgggcagca
    43741 aacttaccgg taaaccgtgg acgctggaat ttcgacacca ttcgctacga ttattacctc
    43801 gatgataatg tcgcctttga agcgtttaaa gcaggtgcct ttgatttgcg tatggaaaac
    43861 gacgccaaaa actgggccac gcgttatacc ggtaaaaatt tcgataaaaa atacatcatc
    43921 aaagatgagc aaaagaacga atcagcccag gatacgcgtt ggctggcgtt taatatccaa
    43981 cgtccggtat tcagcgatcg ccgggtccgg gaagctatca ctctcgcctt tgactttgaa
    44041 tggatgaaca aggcgttgtt ttacaatgcc tggagtcgca cgaacagtta ttttcagaat
    44101 accgaatacg cggccagaaa ttaccccgac gccgcggagc tggtgcttct ggcaccaatg
    44161 aaaaaagatc taccgtcaga agtcttcaca caaatctacc agccgccggt atccaaaggc
    44221 gatggctacg atcgtgacaa cctgttaaaa gccgacaaac ttctcaacga agcgggctgg
    44281 gtgctgaagg gtcagcaacg cgttaatgcc acaacgggtc agccactcag ctttgaatta
    44341 ttgcttcccg caagcagcaa tagtcagtgg gtattgccgt tccagcacag cctgcaacgg
    44401 ctgggtatca acatggacat tcgcaaggtg gataactcgc aaatcactaa ccgcatgcgc
    44461 agtcgcgact atgacatgat gccgcgcgta tggcgggcga tgccgtggcc cagttccgat
    44521 ttacagattt cctggtcatc ggaatatatc aattccactt ataatgcccc cggcgtgcaa
    44581 agcccggtta tcgactcgct gatcaaccaa attattgccg cgcagggaaa taaagaaaaa
    44641 ttactgccgt tggggcgagc actggatcgc gtattaacgt ggaattatta catgctgcca
    44701 atgtggtaca tggcggaaga ccgtctcgcc tggtgggata aattctccca gccggccgtg
    44761 cgccccatct atagcctcgg tatcgatacc tggtggtatg acgtcaataa agcggccaaa
    44821 ctgccgtccg ccagcaaaca gggagagtag atgggcgctt acctgattcg ccgtctgttg
    44881 ctggtgatcc caacattatg ggcgattatc accatcaact ttttcatcgt gcaaattgcg
    44941 cctggcggtc cggtcgacca ggccatcgcc gccattgagt ttggtaatgc cggagtatta
    45001 cccggcgcag gcggtgaagg tgttcgtgcc agccatgcgc aaacgggtgt cggcaatatc
    45061 agcgacagta attaccgtgg cggacgcgga ttagatccag aagtgatcgc tgagatcact
    45121 catcgctacg gttttgataa gccgatccac gaacgttact tcaaaatgct ctgggactac
    45181 atccgctttg attttggcga tagcctgttt cgcagcgcct cggtgctgac gctgattaaa
    45241 gacagtctgc cggtttccat caccctcgga ttgtggagca cgctgattat ctatctggtg
    45301 tcgattccgt taggcattcg caaagctgtt tataatggga gccgctttga cgtctggagt
    45361 agcgcattta tcatcatcgg ctacgccatt ccggcctttt tgtttgccat cctgctgatt
    45421 gtcttcttcg cgggcggcag ctatttcgac ctgttccctc tacgcggcct ggtttccgct
    45481 aactttgatt cgctgccgtg gtatcagaaa atcaccgatt atctgtggca tatcacgctg
    45541 ccggtgctgg cgacagtgat tggtggcttt gcggcgctga ccatgctgac aaaaaactca
    45601 ttccttgatg aagtgcgcaa gcaatacgtg gtgaccgcgc gtgcgaaagg ggtaagtgaa
    45661 aaaaatattc tctggaaaca tgtgttccgc aacgccatgc tgctggtgat tgccggtttt
    45721 ccggcgacgt ttatcagcat gttttttacc ggctcgctgc tgattgaggt gatgttttca
    45781 ctcaatggtc tgggcttact gggctacgaa gcgaccgtct cgcgcgatta tcctgtaatg
    45841 tttggtacct tgtatatttt caccctgatt ggcctgctgc tgaatattgt cagtgatatc
    45901 agctatacgc tggttgatcc gcgtattgat tttgagggac gttaatgtcg cgactcagcc
    45961 ccgtcaatca ggcccgttgg gcgcgttttc gtcataaccg tcgcggctac tggtcgttat
    46021 ggattttcct cgtcttgttt ggtttgagtt tgtgttctga acttatcgcc aacgataaac
    46081 cgttgctggt gcgttatgac ggcagttggt atttcccgtt attgaaaaac tacagcgaaa
    46141 gcgattttgg cggcccgctg gcaagtcagg ctgattatca ggacccgtgg ctgaaacaac
    46201 ggctggaaaa taacggctgg gtactgtggg caccgattcg ctttggtgct accagtatca
    46261 actttgctac cgataagccc ttcccttctc caccctcccg gcaaaactgg ctgggaacgg
    46321 atgccaacgg cggcgatgtg ctggcacgca ttctctatgg cacgcggatc tcggttctgt
    46381 ttggcctgat gctgactctc tgttccagcg tgatgggcgt gctggcgggg gcgctacaag
    46441 gctattacgg cggtaaagtc gatctctggg gacaacgctt tattgaagta tggtcgggga
    46501 tgccgacgct gtttttgatt attttacttt ccagcgtcgt acagcctaac ttctggtggc
    46561 tgctggcaat tactgtcttg tttggctgga tgagtctggt cggcgtggtg cgggcggagt
    46621 ttttacgtac tcgtaatttc gactacattc gtgcggcaca ggcgcttggc gtcagcgatc
    46681 gcagtatcat cctgcgtcat atgttgccta atgccatggt cgcgaccctc acctttttac
    46741 cgtttatttt atgtagttcg ataaccaccc tgacctcgct cgatttcctc ggcttcggtc
    46801 taccgctcgg ttcaccgtca ctcggtgaac tgctgttaca agggaaaaat aaccttcagg
    46861 ccccgtggct tgggatcacc gccttcttgt cggtggcgat attattgtct ttgctgatct
    46921 ttattggtga agccgtccgc gacgcatttg atcctaataa ggcggtgtag catgacgcaa
    46981 actctgttag cgattgaaaa tttgtcggtg ggttttcgcc atcagcaaac cgtacgtaca
    47041 gtagtcaatg atgtttcact acagattgag gctggcgaaa cgctggcgct ggtgggtgag
    47101 tcaggttcag gcaaaagcgt taccgcgctg tcaattttac gcctgctccc ttccccgccg
    47161 gttgaatatc tctccggcga tattcgtttt catggcgaat cgctgcttca cgccagcgat
    47221 caaacgttgc gcggtgtacg cggtaataag atcgccatga tttttcagga accgatggtg
    47281 tcgttaaatc cattgcatac cctggaaaaa cagctttatg aagtgctttc actccaccgc
    47341 gggatgcgtc gggaagcggc tcgtggcgaa attcttaact gccttgatcg cgttggtatc
    47401 cgccaggcgg caaaacggct gacagattat ccgcatcagc tctccggcgg cgaacggcag
    47461 cgggtgatga ttgcgatggc gctgttaacg cgaccggaat tattaattgc cgatgaaccg
    47521 accaccgcac tggacgtctc tgtccaggcg cagattttac agctgttgcg cgaactgcaa
    47581 ggcgagctga atatgggcat gctgtttatt actcataacc tcagcattgt cagaaaactg
    47641 gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg agcaaaatta cgccgctacg
    47701 ctatttgcat cacccactca tccttacaca caaaagctac tcaacagtga accgtcaggc
    47761 gatccagtgc cgttgccaga acctgcctca acgttgctgg atgttgaaca gcttcaggtt
    47821 gccttcccca ttcgcaaagg gattttgaag cgcattgtgg atcataatgt ggtggtgaaa
    47881 aacatcagtt ttacgctacg agcgggtgaa acactgggtt tagtgggcga gtccggttcc
    47941 gggaaaagta cgacgggact ggcgctgctg cgactgatta attctcaggg cagcatcatc
    48001 tttgacggtc agccactgca aaatttaaat cgccgccagc tgttacctat tcgtcatcgc
    48061 attcaggtgg tatttcagga tccaaactcc tcgctcaacc cacgactcaa cgttttgcag
    48121 attattgagg aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca acgcgaacaa
    48181 caagtgatag ccgtgatgca tgaagtggga ttagatcctg aaacacgcca ccgttatccg
    48241 gcggagttct ctggtggtca gcgacaacgt attgcgattg ccagggcatt aattcttaag
    48301 ccctcgctga tcatacttga tgaaccgaca tcatcactcg acaaaacggt acaggcgcaa
    48361 atattgacgc tattgaaatc attgcaacaa aagcatcaac tggcctattt gtttatcagc
    48421 cacgatttgc acgttgtccg cgcgttatgt catcaggtta tcatactgcg acaaggggaa
    48481 gtagtggaac aaggaccgtg cgcgcgcgtg tttgccacac cgcagcagga gtatacgcgt
    48541 cagctactgg cgttgagctg acgcttaaaa aggattgtag tttgaaaagg gttcggcaat
    48601 tgccacacca aaatttttca atcggcatgt tgcagcaaac tcgtcctggc gatttacaaa
    48661 caggcacggc tcaccttcac actccagcac cgaacatgga acttcgatgt cgcttaacgc
    48721 ctggcttagt ttatacatca ccgaccatgc attatcacca tccgggctaa gcaatttaag
    48781 cgccaccagg ctgttgtcac cgcacggtcc attttgcgga atcggttcaa ccttcgaacc
    48841 tgcgaaacca gcagaccagc gatagttctg cggcagtcga tggacgattg agagttgtaa
    48901 tgaagtcact tattttcccc ggaagcacat tcacttcaca atttgtttac atcaatttta
    48961 acacatcatc aacaatccgt ctttatacag atcgtaaaga ttgatgcctc gcctgcgtgg
    49021 cctctatggc tctgatttaa gtaatatctg cgggcgtggt cacgtttgcg gggctatatg
    49081 tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact ttttacataa
    49141 attgatttta cataaaataa acatatatcg ggggaatgat agatttgtgg ttgacatgca
    49201 tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag atcaccgttt tttcggccga
    49261 ctggcgtaca gacagaagag aatggagctg gttgcgcaga atgcaattga ccaaatcatc
    49321 ggccatgcag agttaaaggt cgcaagagaa agcaatgcgc caacaattgc ccctatgcca
    49381 aaacggaagg ttcctgccag cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga
    49441 atgaccgcca tcgcattgga tgacaccatc gacacgcagc ccacaaacgc cgcaacgcca
    49501 accaccagcg accaaaatcc cagccccagc agcgcactga tgaccatcca cgctgccata
    49561 ataaattgta tccacaaccc cgagcggaac atatttaacg cgccaatgcg gcggacgaag
    49621 cggctgttaa agatggtcat cacgaacaga aaaacaatgt ttagcgcaaa gtaataacca
    49681 aagttttccg gcgcgacgtg gttaatttca atataaacaa acggtccggc gcttaagaat
    49741 gagaacatcc cggcaaagct gaaaccactg gcaagcatgt agctcaggac acgtttatgg
    49801 cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt gaaatggctg acgacgctcc
    49861 ggtggtaagg tttctttaat caggaagaaa atcattgccg aagccagaat cgccgctaat
    49921 gccaggatcc agaagatgta atgccagctc agccacacca gcacccagcc gccaactatc
    49981 ggtgccatca gcggtgcaat ggttgtcacc agcatgacaa acgacatcat ccgcgagaac
    50041 tcttctttcg ggtaaatatc gcgcatcagg gcgttaatga ccacgctggc cgcagccgca
    50101 gccagcccgt ggaagaaacg catcacaatc agctgatcga tggtgtttgc caacgcacac
    50161 gccaccgcgg cggcggcaaa caccagcgta ccgccgagca ccaccggctt acgcccgaag
    50221 ctgtctgcca tcggcccgta gattaactgc cccaacgcaa agcccagaat ataagtactg
    50281 agggtcatct gcgtactgcc cgccggtacg ccaaactgcg ctgaaattac cggtagcgcg
    50341 ggcagataca tatcaatcga cagcggcatc aacatggcca gcaggccaag gataaaaaca
    50401 atagcaaacg acgaatgctg tcgggtggtc acaacgggct cctgaaagtc attgaaaagt
    50461 tagacgacgc tggcaatttc ttcttcagtt aacggacgat attcaccggg ggctaaatca
    50521 gcatccagcg taataccgcc aatacgttca cgatgcagct caaccacgtg gttacccacg
    50581 gcggcgaaca tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag acgaacctgc
    50641 gttggggtaa tcacttccag caccgcaggc ttagtgagat ctttttcgtt atgcagctgc
    50701 acgcctttag caaattgctc tgccgtatcg tcagctacag gtgattccag tgtcaccaga
    50761 taggtcttct cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg accatcatca
    50821 gtcatcagca ccagaccggt ggtatcaata tccaaccgcc ccgccgcatg cagtttccac
    50881 gctaccggtt catcaagaaa atagagcacc gttgggtgat cagggtcgtc cgtggagcaa
    50941 acatagccct gaggcttatt gagcatgaag taacgtggac cgtgttgctg cgccagcggg
    51001 ttgccatcgt aagcgacatc atgttcagga agcagtttga acgctgcatt acggacgatt
    51061 tcgccatcga cggtgacacg attgccgcgg atttcacgcc cggcaatagc acggctaacg
    51121 ccgagttgct gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaacg
    51181 gaggtcgggc attgacccga aaatctgaac tgttgtctgc ccagtatagc ggtctaataa
    51241 catccctcaa gggaaaaaga tccatggcat actattagca gaataatcta cctacgcgag
    51301 accatgattt ttacacttcg cccatatcag caagaagccg tggatgccac gctcaaccat
    51361 tttcgtcgtc ataaaacccc tgccgttatc gtgctgccca ccggcgcagg taaaagcctg
    51421 gtgatagcgg aactggcacg gctggctcgt ggtcgcgtgc tggtgctggc acacgttaaa
    51481 gaactggtgg cgcaaaacca tgcaaagtat caggcgctgg ggctggaagc cgatattttt
    51541 gccgccgggc taaagcgcaa agagagccac ggtaaagtgg tatttggcag cgtgcagtct
    51601 gtcgcccgta atcttgatgc ctttcagggt gaattttcgc tgttgattgt cgatgaatgt
    51661 caccgtattg gtgacgatga agagagccag tatcagcaaa tcctcactca cctgacaaaa
    51721 gtgaatcccc acttacgcct gctggggctg actgccacgc cttttcgatt gggcaaaggc
    51781 tggatctacc agtttcatta tcacggcatg gtacgcggcg atgagaaagc ccttttccgt
    51841 gactgcattt atgagctgcc gctgcgttat atgattaaac acggctatct gacgccgcca
    51901 gaacgactgg atatgccagt agtgcaatac gatttcagcc gcttgcaggc acagagtaac
    51961 gggctgttca gcgaagctga tctcaaccgt gagctgaaaa aacagcaacg cattacgcca
    52021 catatcatca gccagatcat ggagttcgcc gaaaagcgca aaggggtaat gattttcgcc
    52081 gccaccgttg aacacgcaaa agagattgtg ggattactgc ctgccgaaga tgctgcactg
    52141 attactggcg acacccccgg cgctgagcgc gatgtgttaa ttgaagattt taaagcccag
    52201 cgttttcgct atctggtcaa cgtcgcagta ctgaccaccg gatttgacgc cccgcacgtc
    52261 gatcttatcg ccattctgcg ccctaccgaa tcagtgagtc tttaccaaca aattgtcggg
    52321 cgcggtctgc gtctcgctcc gggcaagact gattgtttaa ttcttgatta tgcgggtaat
    52381 cctcacgatc tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga caacgttccg
    52441 gtacaggttt tctgccctgc ctgcggtttt gccaacacct tttgggggaa aacgaccgcc
    52501 gacgggacat tgattgaaca ctttggtcgc cgctgtcagg gatggtttga agatgacgac
    52561 ggtcatcgcg aacagtgtga cttccgtttc cgttttaaaa attgcccgca atgtaacgca
    52621 gaaaatgata ttgccgctcg ccgctgccgc gaatgtgaca cggtactggt tgatccggat
    52681 gatatgttaa aagcggcgct acgactgaaa gatgcgctgg tattacgctg tagcggcatg
    52741 tctttgcagc atgggcacga cgagaaaggc gaatggttga aaatcaccta ttacgatgaa
    52801 gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc ccgcccagcg aactgccttc
    52861 gagcagcttt ttatccgccc gcatacgcgc acaccgggca tccctctgcg ctggatcacc
    52921 gccgccgata tcctcgccca gcaagcctta ttgcgacacc cggattttgt cgttgcccgc
    52981 atgaaaggtc agtactggca agtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt
    53041 cgggcgcacg aattacgcgg ttaatggtag ttttgattga tgtataaagc gattgagtat
    53101 agaatctcgc ccgcttttgc atacgcaaag cagatcactt acctgttgct gggtcgcctg
    53161 tagcaggatt aatttaagag agaaagaaat gtttactatc aacgcagaag tacgtaaaga
    53221 gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg caatcatcta
    53281 cggtggcaaa gaagcgccgc tggctatcga gctggatcac gacaaagtca tgaacatgca
    53341 agctaaagct gaattctaca gcgaagttct gaccatcgtt gttgacggta aagaaatcaa
    53401 agttaaagct caggacgtac agcgtcaccc gtacaaaccg aagctgcagc acatcgactt
    53461 cgttcgcgct taattgctga ataagttgta aaaaaccccg ctccggcggg gttttttgta
    53521 tctgcagatt atgcctgatt acggtattgc tattttttgc aggccagata aggcgttcac
    53581 gccgtatctg gcattcagaa cacaaaaccg attaattgcc gccagacgtc cggcgctgca
    53641 attggtcgcg caaattcggc ggcgtgcctt taatggtcaa ggtatcggtt gctggatccc
    53701 agaagatgcg ttcacctaac agaatcgcat caaagttaat agtcaagcca ccgccgctac
    53761 cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt
    53821 cgtagccttt ttcagccgca aactccgtga agctcacttc gcttacgcct gccagctctt
    53881 tcgatagcga ctccagttca atctcttcgc ctgcctgcag ctgctcatta cagtagctat
    53941 aaacctgctg gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat
    54001 catcaaccgc ttgcaacagc ccacggtttt gcgctttcgc gtttaaccct tcgctggccc
    54061 cgaggaaatc cataaagaaa tccgccactt tgcgccctac tcgccctttc aggaaagtga
    54121 gatagcgggt ggactctgga ttggtttccc attcggttaa atcgatacgc gcaacaatat
    54181 ccgcatgatt gatatcaaga taatgggttg gattgatgtc gagattttcg ttaacgcgca
    54241 tactgctcag gttgctcaga actgccacca gcagatactc caccgccaga taacggtagt
    54301 ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc
    54361 gcccggttgc cgcgcggcta aatgccagaa agtcctcttc gccctgacgc tgtaagcgca
    54421 gcgtctgcgc cagttcactc tcttcgctaa acaagccgta agctttattt ttggcgctat
    54481 agacccgatg cagttctgcc accatctcaa cgacggtttc tgtcggttcc agcaatgaat
    54541 cgcgcaacac cagctcaagg ttctgctcat cacgcttgat aagctggtgc agagcaatct
    54601 ggttgatatc cagactcatg ataaactctc ctttaagacc gggcggtatt caaccaccgc
    54661 cggttaagcg acgcaataaa agatggcggt cattttacag aaggcagcgt aacgagaatg
    54721 atttaacgca taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat
    54781 caactaactg atttcgattt atgccacaaa tttcccgcta cagtgatgaa caggttgaac
    54841 aattgctcgc agagctgctc aacgtactgg aaaaacataa ggctccgacc gacctttccc
    54901 tgatggtgtt aggcaacatg gtcactaacc ttatcaacac cagcattgcc ccggcccaac
    54961 gccaggcaat tgccaactct tttgcccgcg ccttacagtc ctctatcaac gaagacaaag
    55021 cgcactaagg gaaacagata acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa
    55081 gtctcccaga tggtcagttg ggggcactgg tttgcactgt tcaatattct gctttcgctc
    55141 gtcattggca gccgttacct gtttatcgcc gactggccga caacgcttgc tggtcgcatt
    55201 tattcctacg taagcattat cggccatttc agcttcctgg tgttcgccac ctacttgctg
    55261 atcctcttcc cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc
    55321 attctggcaa cggcgggaat gacgctatta ctgatcgata gcgaagtctt tactcgtttc
    55381 catctccatc ttaatcccat cgtctggcaa ctggttatca acccagacga aaatgagatg
    55441 gcgcgcgact ggcagctgat gttcatcagc gtgccggtta ttttattgct tgaactggtg
    55501 tttgcgacgt ggagctggca aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc
    55561 ccgctggccg cattcttatt tatcgccttt atcgcctcgc atgtggtgta tatctgggcc
    55621 gatgccaact tctatcgccc gatcaccatg cagcgcgcta acctgccgct ttcgtacccg
    55681 atgacggcgc gacgttttct tgagaagcat ggtctgcttg atgcgcagga gtatcaacgc
    55741 cgtcttattg agcaaggtaa tccagacgcc gtttccgttc agtatccgtt aagcgaactg
    55801 cgctatcgcg atatgggcac cgggcagaat gtgctgttga ttactgtcga tggcctgaac
    55861 tactcacgct tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg
    55921 ttcacgcgcc atatgagctc cggcaacact acagacaacg gcatctttgg cctgttctat
    55981 ggcatctcgc cgagctatat ggacggcatt ctgtcgaccc gtacgcctgc ggcattaatt
    56041 actgcgctta atcagcaagg ctatcagctg gggttattct catcagatgg ctttaccagc
    56101 ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc
    56161 gacgagcaga ccgccacgca gtggatcaac tggctgggac gctacgcaca agaagataac
    56221 cgctggttct cgtgggtttc tttcaatggt actaacattg acgacagcaa tcagcaggca
    56281 tttgcacgga aatatagccg ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc
    56341 aatgcactgc gtgattctgg caaactggac aatacggtgg tgattatcac tgccggtcgg
    56401 ggtattccac tgagcgaaga ggaagaaacc tttgactggt cccacggtca tctgcaggtg
    56461 ccattagtga ttcactggcc aggcacgccg gcgcagcgta ttaatgctct gactgatcat
    56521 accgatctga tgacgacgct gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa
    56581 tattcgcaag gtcaggattt gttcaaccct caacgccgtc attactgggt taccgcagcg
    56641 gataacgata cgctggcaat taccaccccg aaaaagacgc tggtactgaa caataacggt
    56701 aaataccgca cttacaactt acgtggtgaa agagtgaaag atgaaaaacc acagttaagt
    56761 ttgttattgc aagtgctgac agacgagaag cgttttatcg ctaactgatt aattataaat
    56821 cagttagcga aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga
    56881 gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca
    56941 aatcctctcg tgccgaccaa aaatcccaag aaaaaaccaa cccttacggt tggttttttt
    57001 atatctgcaa ttaattcgat aaacagaccg tgacacatca cagcctgttt attttctgtt
    57061 atcagaacgt ccagaccaca cccgcctgag tattccacgg cgattctacc cctgcgccat
    57121 tgccatagct gactgacaaa tgaccgctta acgtcggggt aaacgatgac cttataccag
    57181 cctgataaac gccactggtt ccagccacat cattattaaa tttgccatcg tcattaactt
    57241 tcacctggtt agaatcggcg tattcctgac gcacggccgc tttcagccag ggttccagcg
    57301 tcgtaccgtt ttgcaggtcc atgtgatagc ttaccgccgt tcccgcttca gcgcgtaata
    57361 tccgggtatt tcccacatcc gcgcgcatgc cgtttgataa cgtgtagtcc tgaccatctg
    57421 tggtaaagcc ggtaaaggcc agatagggtc taacactcca caatccgtca acccaacgga
    57481 acccgctctc aacatgagca cccgcgccgt tactattgta atcgccaaac gctgttgccc
    57541 cattactcat cttgccatgg atggtgttag caaaacggtc aactttcacc accccatcaa
    57601 cataggcacc gttctgatgc tcccaaccgg cataagcccc cagggtatag ctatcgatat
    57661 tacctttgcc gccgcgatca aaaccaatat cagaatgaga gtaaccaaag atcaagccgc
    57721 gaattgtact gctttcttca cgggagaaac ggctatcgat accgagcgtc aggcccgtca
    57781 atgtttgctc aaaaccagct cccgcatcag tggtcacgtt gttgcgggtg ttaattgccg
    57841 aactccacat cgccgtatcg taactaacgc cttttacgct accaagacgc tcacgcacgg
    57901 tgtccagttc tgcatcaaat accagcggtt gtgcggccgc catattcagc acatcagtgg
    57961 ttgaaggggt aatttgcgcg cgattctctg ccagactcca gctatggttg ccattatcca
    58021 gcaaggtata ttcatacgta ccgatatcaa caacgcctcc ggcattgccc aacgtaaatg
    58081 cagcatcacc gccgcccgtt gttaccagtg taaggctatc tcctgctgcc gggctggcac
    58141 cggtgtccgt cacgaatatt ttgaaatcac ctgttgcctg accggtgacg ttgagctgat
    58201 cgctctgatg attagccata tcggtacgca tataaaaatt gccgtttccc gacagggtat
    58261 tggtggtcaa cgtaatgaaa ttttccgcac ttgccgtcac tgatgaacga gtcactggct
    58321 catccatcat aatcacattt gcgtcagaca tcgcaaggtt ggctaagttg aggagggtgt
    58381 tttggttcgt cgtagtgata tcggcaatga tggtccatac gctattttcg agcgaaacgt
    58441 ccgctttgct ggtatccacg gcaccatgcg ttctaaaaga agcgccctca gaaaccacta
    58501 ccttacccac caacattgcg ggtgcataat ttatttgcgg cttcatgacc tcaagaatgc
    58561 catcattccc tctgactgaa acgttaacca ttgtgccagc ccagacgtta gcgcggccat
    58621 tgttgataac catattttca gagatggctt ttgaggaata cgtgatactt ccattctgat
    58681 atgatcgacc gagatcatat accgcatttg cctgcacttt cgtaccagta tcctttccca
    58741 gcgattgcat agtggcatgc ttatcaagga tagtatcaat ggcctgcgtg tcctccataa
    58801 caatgagccc ggaaccatca tccagttcat aattttttga cacaccatct tttatactaa
    58861 attggccttt actgtttgga ccactcactt ccagcgcatt cgttgagaca attaattttc
    58921 cgcctgcttt tttatcaacg ccagttacag tcccgccatc cataacctcc agtaagccgc
    58981 cactatctac agtagtatta taagcgaagt cattttcttc aactcgcaaa cttccgccgt
    59041 tttccaacag catattgtta gcaataccat tcttgatttc gaactgaccg agacggtttg
    59101 ttccgaatac ctccatgacc cgcgtggttg ctttaatagc accgcctgct ttctgatcta
    59161 ccgcaaatgc aaatcccccc tctttaacct ccagtaatcc tcccttttcg atagcggcaa
    59221 agacggtccc cccatctttt tcaacgatta agcgcccgcc tttaatcgtg ggatcattcg
    59281 ccgcgccata aacctgtagc actccattat tgatcgtagt ctggtttgcc gcgccaccca
    59341 tttgaacaat ttgataaccg tcatttaata ctgagtattc tgcggcgcca ttcatttcaa
    59401 tgtactgata ccctccgtta atttcagtat tattacttac accaaattct tttatatgct
    59461 gttctccgcc aggattgatt ttcgtattat tggcggtccc ataaaccagc tggatatctt
    59521 tttccaggac aacaccatca actgtctcac catactcaac agttgatgcc aatgctatgg
    59581 gtgaatataa cgctgtagcc accagaacag atattgcgga acgacaaaga gaaacagaac
    59641 cagattgatg cattgagctt tcatcctatg aaattaattg ctgttaaaag cattgggtac
    59701 agaaaatacc catagctcca tacccggagt cagtttttaa aaactgttta aagaaatgca
    59761 caagtattgt gattgatttt ttagttgttt ttcttgatga gaagctgatg caaaattccg
    59821 tctttataat gaaaatgatg ccaaagcgaa cgacaaggtt gtagttttca ctacatgtcc
    59881 atacataaaa tggggtaaca ttcacgcgcc tggtagcgtt accaacgcta cgctcaaaca
    59941 taatgattct aataaaacct caggagacta ctatgcctga agcaacgcct tttcaggtga
    60001 tgattgtgga tgatcatcca cttatgcgac gcggtgttcg tcagttactg gagcttgatc
    60061 ctggctttga agtggtcgcc gaagcgggcg acggcgcgag cgctatcgat ctggcgaata
    60121 gactggatat cgacgtgatc ttgctggatc tcaatatgaa aggtatgagt ggcctggata
    60181 ctctcaatgc cttgcgcagg gatggcgtta ccgcgcaaat tattatcctg accgtatccg
    60241 atgcctccag cgatgtcttt gcgctgatag acgcaggcgc agacggttat ctgttgaaag
    60301 acagcgaccc ggaagtattg ctggaagcga ttcgtgccgg agcgaaaggc agcaaagtct
    60361 ttagcgaacg cgtcaatcag tacttacgtg aacgtgaaat gtttggcgcg gaagaagatc
    60421 ccttcagcgt gctgacggag cgcgagctgg atgttctgca cgagctggca caggggctgt
    60481 caaataaaca gattgcctcg gtgttgaata tttcagagca gacagtaaaa gtacatattc
    60541 gcaatctgct gcgcaaactc aatgtccgct cacgcgtggc ggccaccatt ctgttcctgc
    60601 aacaacgcgg ggcacaataa aaatagcccg atggatttat catcgggctg agatttatga
    60661 caaacgcaaa actgcctgat gcgctacgct tatcaggcct acgtggatcg atcaatttat
    60721 tgaatttaca caattttgta ggccggataa ggcgttcacg ccgcatccgg cataaactaa
    60781 gcgcactttg tcaacagtct agcccgatgg catcaccatc gggcctcttt ttatttactc
    60841 tcctgcggcg acaaatgttg catcgcctgc gcgatactac gttcaatcac cgcacggcga
    60901 gtatcgttgg caggtaagag tttcaacatc atctcccacg cggcaacggc ttcgccaaat
    60961 cgctgctgct caaaggcatt aaacgcatac atgcttagca cacggatatt gctatggtcc
    61021 gttctcacca gctgacgtag cagttcaccg ccgaggcggt tgtcgttggg atcagatgaa
    61081 cgagtcaacg cttcagcgta tcccagtgca gcatcactgt ttttcggatc gaggcgatac
    61141 gcagtggcgt atgcatcggt ggcgatactg gcgttaccca gcgccatgcc aacgcggccc
    61201 aacataatcc agccttctat atctcccgga tttttttgca gttgagtacg catccccagc
    61261 gcaagacgcg acatctcttc ttcgttgagc ggatcggctt tcggatccag cgccctgtcc
    61321 agcaacgccg gagtctgtgc cgtggcctgc tgccagattt tcacctgctg ataattgcca
    61381 gtctggtagt agctgacgcc agccacaatt aacgccacca caatacccgg cagataaaca
    61441 acatatccgg cacgcttacc ttccggcacg ctttgttcag gaaacgcttc cggcaccacg
    61501 cgtacccgac gccgcgagcg ggcgtatatg acccaaccgc caatgccaat agccactact
    61561 ggcagcaccc acagcagcac ggtcagcggc gttaacggcg gatcgtaagt gacgaagttg
    61621 ccgtaacgcg ccaccatata atcgacaatc tcttttttac ttttaccttc ctgcatcagt
    61681 tcatacactt tctgacgcag gtcggtggca atcatcgagt tggaatcggc aatgctgttg
    61741 ttctggcatt tcgggcagcg cagttcttca gtgagctgac ggaactgttg ttcctgtgct
    61801 tcatctttaa actgcaacac atcgatggtc gccagcgctg agccggagat catcagcatc
    61861 agcacgccca ataaaaacct cattgtgcgg cctccttact gtatttctcc cacagcggct
    61921 tgatctcttc ttcccagacg cgaggattca gatcgcccgc atggcgatag cgaatgatgc
    61981 cgttgccgtc aataagaaac gtttctggcg cgccatagac accgagatcc agccctaaca
    62041 tgccatcgcc atcaaacagg cttagcgcgt aaggattgcc cagctctttc agccagctga
    62101 ttgccttctg gcgatcgtct ttatagttca tgccgaccac gcggatgccc tgcgcagaaa
    62161 gctgattcag atattgatgt tccgcacggc aggtcggaca ccaggtcgcc cagacgttaa
    62221 gcagtactgg tttgccctga gtcagcacat ccgcctgata aaactgcccc ggattgtcca
    62281 gtgattcgag acgaaacttc ggcacaggct tgccaatgag cgccgattcc agattggtcg
    62341 gatcatcccc ttcggcatta cgcgccagct gccacagcag cgccgcggca atcgccagga
    62401 agataatcaa cggaattaac aatactttgc gcttcatacg gcctccggcg cagttttttg
    62461 cggactcacg cgcttacgat agcgaggatc aaacagacac agcagtccgc ccaacgccat
    62521 catcagcccg cccgcccaaa tccagcgaac aaatggtttg tagtaaagac gcacggccca
    62581 cgcgccgttt tccagctctt caccgagcgc ggcgtacagg tcacgcgtga tgccgccgtc
    62641 aattgccgct tcggtcatta tcgacccggc agtgttgtaa tagcgttttt ccgcatacag
    62701 cactgtttcc ggtttaccct ctcgcgttac gccgatagtc gccacaccgc cacgccagtt
    62761 cgggccagtc acctctttga catcacggaa ggtgaagcga tattcatgaa tatcgacgct
    62821 atcgccggac ttcatgcgca catcacgctc aacgctgtag ttctgactaa aggcaatgcc
    62881 aacaattgtc actgccagcc ccaggtgagc agccaccatc ccccaataac tgaaggtggt
    62941 tttcgtgccg cgtgaaatac gcagcacagc ttccgcaatt gccagcaccg caatccagca
    63001 ggccattgcc aggccgagca ccgtcattgc cacaactttg ctttcgaaca gccacggcaa
    63061 cagcagagac agcaccagcg tagagatgaa ggcgataatc aataaattgc ggattttacg
    63121 tgggcgatcg cgcccccagc gcaccagagg accgacaccc agcagtagcg caaacggcac
    63181 catcagccag gtaaacatgg tgttgaagaa cggttcgcca atcgaaatac tgcccagtcc
    63241 cagttgcttg tgcaccagcg gcagcagcgt ccccagtaat accaccagca tcgcggcgac
    63301 cagcaaaacg ttgttcgcta acagcaaaga ttcccgcgac cacagcgcat tgtttacgcg
    63361 tgagcgaact ttgtgtccac gcgcggcaaa cagcagcagc gaaccgccaa tcaccagcac
    63421 cataaaggcg aggataaaca taccgcgcgc cggatcagac gcaaacgcgt gtaccgatac
    63481 cagcacgccg gaacgcacga ggaaagtccc caacagacac aacgagaagg cactgattgc
    63541 cagcagtaat gtccacgctt tgaagctggc gcgttgttca gtgaccgcca gtgagtgcat
    63601 cagcgcagtc cccaccagcc acggcataaa cgaggcgttt tctaccggat cccagaacca
    63661 ccagccaccc cagccgagtt cgtaataggc ccatgcggaa ccgagcacga tgccgagcgt
    63721 caggaagatc cacgctgcca gcgtccacgg acgagtaaaa cgcgcataag tgctgtccag
    63781 acgcccgctc agcaaagaag caatggcaaa agcaaacgcc accgagaaac ccacgtaccc
    63841 catataaagc agaggcggat ggaagatcag ccccggatcc tgcaatagcg ggttaagatc
    63901 gcgaccttca atcgggaagt tcggcaacgt gcgagagaac gggttagagg taaagagaat
    63961 gaacagcaaa aagccgacac tgaccatccc cattatcgcc agtacacggg ccacaatatc
    64021 cagcggaata cgctgactaa aaatcgccac cgcaaaggtc cagccgctca tcagcagcac
    64081 ccacagcagt agcgagcctt catgcgcgcc ccaggtagcc gccacgcgat accacaccgg
    64141 aagctgggta ttggagttgc tggcaacata ggtgacggtg aagtcgttga ccacgaaagc
    64201 attgaccagc accagaaatg cgccagccac agacataaac agcagccagg caaacaagcg
    64261 ggaagacgcc atcatgcgcg catctccgcg cgccacgccc catagcggat acacggacag
    64321 cagcagcgca attcccagcg ccaggcacag cagtccgtta ccaatttctg gcatcatgat
    64381 gctgggtcct tataaacact cgccgggcga cggtggttag cttccatcgc tttctcaact
    64441 tctggcggcg tatagttttc atcgtgtttc gccagcactt ctttcgcgag gatatgattg
    64501 cctttttcca gttcgccctg caccacaacg ccctgccctt cacggaacag atccggcaaa
    64561 atgccttcgt aagagacatc cactgagcct tcagcatcgt aaatggtgaa ggtcactttc
    64621 agcgaattgg gatcgcgctg cacactaccc ggcatcacca tcccgccaac gcgcagacgc
    64681 tgaccgactt ccggcatttg ctgagtttca cgcttgccgt agagaatttc ccccggcgta
    64741 taaaagagat cgatattcga gcgcagcgca tatagcacca gaccgatagt cagcgccagc
    64801 cctgccaaca cggcacaggc aatccacaag cggtttttac ggcgaatatt catgcagcct
    64861 cctgctgttg cgcagcacgt aaacgcgcct cacgcgcccg ctgttgcgcc acgccacgca
    64921 gaattgcgcg atgttgcatc accgagtgca cgaccaaaac caccagcgga ataacggtca
    64981 tcaccaccgc cagccagaca aaaaaggcgt aaccgcccat tgcgaaaaat tcattccagg
    65041 aagcaaatgc aggggtcatt tacggcctct tttcagtatc agttcactca cccacggacg
    65101 gcgtttttcc atcagcaaaa tcaaattacg catccgcatc agcgtcagcg tggcagacag
    65161 gagcaggaag ccaaaaatcg accagcgcag cggcgaacgc atcgccggat cgatactttg
    65221 ctgcatccgc gttgatccct gatgcagggt gttccaccac tccacggagt aatgaataat
    65281 cggcagattc accacgccaa tcagcaccag gatacctgcc gcacggcccg ccagacggcg
    65341 gtcgtcgaag gcgtgccaca gggcaatcac acccacatac aaaaacagca gcaccagttc
    65401 agaagtcaga cgtgcatccc atacccacca ggtgccccac atcggttttc cccatgcaga
    65461 gccggtaacc agggcaataa aggtaaacac ggcaccaatg ggggccatcg ccgccaccgc
    65521 caggttggcc attttcatct gccagacaag gccaataaac gctgccactg ccattgatgc
    65581 ataaatgccc atcgaccaga tcgccgcagg cacatgcagg tagataatgc ggtagctatt
    65641 tccctgctga taatcagccg gagcaaagcc gaatccccag atccagccga cggtaagcac
    65701 gaccacactg gcaattgcca gccacggtat aaaccagcca cagatttgat acagccgtgg
    65761 tgggatcgcc agttgatgca gtgttttcca catagtttcg ataccagact cgaacaaaaa
    65821 tcagtaatcc agcgttattg aatgctgatt cgtaacgctg ccgccgtcgc aaaaggactt
    65881 aatgtcgcgg tgcctgccag caacgcgcct aaaattgcca gatacccgtc aacgggcaaa
    65941 tgcatagaag ccgcgtccat cgcggcggtg gcaaagatga gtaatgggat agtcagcggt
    66001 aacaccagta tgctgagcag cacaccaccg cgcttaagtc ccactgtcag cgccacgccc
    66061 ggtgcaccga gaaagccaag cgtaggcgtt cccagcagca gcgtcagcgc catcacttgc
    66121 cagccataaa catccattcc cagtagcatt gctaccagtg gcgaaaggat gagtaacggc
    66181 agaccggtta ccatccagtg cgccatcacc ttcgccagca caacggcggg caagggtaac
    66241 ggcaacaaca tcaattgttc aagactgccg tcctgcaaat cgtcacggaa cagtcgttcc
    66301 agcgccagca aggatgaaag cagcgcagca acccagataa tgcccggtgc aatacgcgcc
    66361 agcagttgcg gctccggacc gatactgagc ggaaaaaggg taattacaat caggaagaac
    66421 cacagcgggt tggcgatttc ggcgctatgg cgaaacgcta cacgcagctc aagacggaaa
    66481 atgcgccaga acatcatgcg gccctcgttt gcgtcagtga aatgcggcga attttacttt
    66541 cagcaacgtt gagcggctgg tgggtagtca gaatcacaat ccccccctgc tccgtatgct
    66601 gcgccatacg ctgggtcaga cgatcgacac cgttaacgtc aatcgcggta aaaggctcgt
    66661 cgaggatcca taacgtggca cgggtcagcc acagacgcgc taaagcgacg cggcgttgtt
    66721 gcccggccga gagctgattt acaggaatat cttcgaatcc ggcaagcccg gcctgcgcca
    66781 gggcttccag acattgtgcg gtatcgccat cgcgatgata aaagtgcaga ttttctaacg
    66841 ccgtcagccg ggttttgatc cccggctgat ggcctatcca taacaggttt tgatggtagc
    66901 tgtcgcgtac ctgatgcaag ggctgccctt gccagagaac ctcgcctgcg tcagggcgag
    66961 acaaccccgt cagcaaacgg agaagcgttg tcttccccgc gccgttgcta ccggtgattt
    67021 gtacccactc tcctgcgttc agcgtaaatg acaagccact aaataaggtt cgttcatccc
    67081 gctcacaaag taactctctg gcttcaagca tacccacgca ataaccctgt taaaaacctg
    67141 gctcgacttc acgcatatcg ggcagcttgt gcgctatccc tttatggcaa tcaatacagg
    67201 tttgcccatc tttcaccgcc tggtcatgca tcttcgcggc aaccgatttc tgggcggttg
    67261 tatccatata ctcgaagttg tgacagttac ggcactcctg cgagttattg tccttcatgc
    67321 gccgccactc attctgtgcc atcgtcagac gatgagcttc aaatttctgc ggcgtgtcaa
    67381 taacgccaaa aattttacca tacagctctt tacttgcttt gagcttgcgt atcatcttcg
    67441 gcacaaactc gtgcggaacg tgacaatccg gacaggtcgc acggacgccg ctacggttgt
    67501 tgtagtgcac ggaatccatg tattcctgat acaccgtgtt gcgcatttcg tggcagctaa
    67561 tgcagaactc ttcggtattg gctttttcca tcccggtgtt aaagccaccc cagaagacga
    67621 tgccgccaac aaaaccgatc aacagcagcg tccccagcgc cagacggctg ggggtacgcc
    67681 accatttcca caggcgctta atcagaccag gcttacggtc agaatttccc ataataacct
    67741 cttatttccc gtaacctttt gatggggtaa aggtattccc cacgattggc gcggtatcgg
    67801 cctgcggtac gtgacattgc agacagaaat aacgacgcgg agccacttcc gcgcccactt
    67861 tgccgtcgct gtccataaag tgagtaggac tgatacgcgg cgcgccagtg gtgcgatagc
    67921 tttcgacacc gtggcactgc aagcagcggt tggtattggt cgttacctga taaccttcaa
    67981 cgctatgcgg gatcattggc ggctgattca catagttcag cggcatccgt tcctgctctt
    68041 ttggcatccg aatggcccct tcctgtgtcc cggatacttc cggcgactgg ctaaaatcga
    68101 cgccattagc cgcccaaacc gcgccgctta ccaccagggc cagcatcgcc gtccattgac
    68161 acagcgcttt cttcaggtca tggcttttca tgatttcgct cccgaactcc atcgtgtagt
    68221 tattgtaaat acatcctcag aacagacatc cacgcagcga ccgcacgtca tgcaatcgcg
    68281 gctggtgacc tgcaccgggc tttgttcatc cagcaccggg gcacgtagca catgcggttc
    68341 cgggcaaaca tgaaaacaat ccatacagcg gttacatttc tgccgatcgg tggccgcaac
    68401 ggtaatcaca cctttgctac ccagcacgcc atacagcgcg ccaaccggac aaatgtgccc
    68461 gcaccagccg tgttcaacga ccagtagatc aaataaaaac agcgcgagaa taagcagcgc
    68521 gccgctgccg aagcccatta ccaggctacg ccccatcaga gaaacggggt tgatccattc
    68581 ccaaatgagc gtgccagtta aggccgatcc caccagaatg accaccaaca gcacgtagcg
    68641 aatgtggcga gggatcgtcg cagactgatt gaggtcaaag cgcctgcgta accagttcgc
    68701 taagtcggta atcgggttca gcgggcagac ccagctgcaa aataatcgct ttcccgccag
    68761 ggcgtagagc acggtgataa tcaccgcacc ggtcaacgcc accgtggcag gcagatgacc
    68821 actggcaaga ctttgcagcg tcatcagcgg atcggtcagc ggaacggtgt cgaataacag
    68881 gctactgctg tagttgccgt gcaagatcca cacaccaaac catggaccgc tcaaaaacat
    68941 ccccagcacg aagaactgac aaaggcgacg taacaccagc caacggtgac tgcgccacca
    69001 gccttttttc tccagcgcct cgcgcccggc gtcacgttta cgatttgcca ttgttcccct
    69061 ccagccagcc gaagcggtaa tggtgaccta actccccttt cgccagtgac agcggtaaca
    69121 ccttgattgc cggttgttcc agcacgcata ctttttcgca cttaccgcaa ccggtacagg
    69181 cgtcgctgtg aaccgtcggc agaaagcggg cgtgcttacc ggtacgcgtg ttgcgctcca
    69241 gctccagggt gatggcctca tcaattttcg ggcattcgcg ataacaaaca tcgcagcgca
    69301 gcccctgaaa gttgagacag ttttcctggt ccaccagtac cgccagcccc atccgcgcgt
    69361 cgtcgatcga ttcgatctca cgatccagcg caccgctcgg gcacactttg gcgcacggaa
    69421 tgtcctcaca catttcgcaa ggaatatccc gtgcgacaaa atatggcgtg cccgccgaca
    69481 gaccagaggc cagcgtcgcc agttttaagg tgtcgtaagg gcaagcctga acacactgac
    69541 cgcaacgcac acaggcactg gcaaaggcgt tctcgtttat ggctccgggc gggcgcaacc
    69601 gcacgccaga tgcgcgtgcg gtttgctgtt gtaaccccag cgccacaccc acggcagcca
    69661 gcccgcctgc tgtgcgaaca acatcgcgca gaaagcggcg gcgaccattt tgaggtttcg
    69721 ctgaccggga cataatggct tacaccttct ccagtttgac cgcgcacttc ttgaagtccg
    69781 tctctttcga gagcggatcg gtcgcatcca gcgtcagttt gttaaccaac tgtgcggcgt
    69841 cgaagaacgg catgtacacc aggccctgtg gcggacggtt acgaccgcgc gtttcaacaa
    69901 tcgagatcac ttcgccacgg cgagaaacca ctttcacttt gtcgccacgg cgcagatcgc
    69961 gcgctttcgc atccagcggg tgaataaaca ggaccgcttc cgggaaggcg cggtgcagtt
    70021 ctggtacacg gcgagtcata ctgccggtgt gccagtgctc cagaacgcgt ccggtagaga
    70081 gccacaggtc gtactcttca tccggtgctt ccgccgccgg ttcgaatggc agtgcgaaga
    70141 tcaccgcttt gccatccggt ttaccgtaga acttatagcc ttcgcccgct ttcacgtacg
    70201 ggtcgttacc ttcgctgtaa cgccactgcg tttctttacc gttcaccacc ggccagcgca
    70261 gaccgcgcgc tttgtggtag tcatcgaacg gtgccagatc gtgaccgtga ccgcgaccaa
    70321 accatgcgta ctcttcgaac agcccttttt gcagatagaa gcccagctcg cgggattcat
    70381 cgttcagctg atcttccgcc agttcggata ccgggaattt gctcacttcc ggtgtggcat
    70441 acagcacttc gtacagcgtt ttgccacgca gttccggttt cttcgccagc agatcttccg
    70501 gccatacttc ttcagttttg aagcggcggg agaactggac taactgccag agatccgatt
    70561 tcgcttcgcc cggtgcctgt acctgttgac gccagaactg agtacggcgt tcggcgttac
    70621 cgtaagcgcc ctctttctct acccacattg cggtcggcag gatcaagtcg gcggccagcg
    70681 cactgactgt cggatacgga tcggagacga tgatgaagtt gcgcggatcg cgccagcccg
    70741 gcatacgctc ttcattgatg ttcggcccgg cctgcatgtt gttggtacac atggtccagt
    70801 aaacattcag tttgccgtct ttcagcgcac ggtcttgcgc caccgcgtgc agaccgattt
    70861 tcgccggaat ggtgccgctc gggatattcc acttcttctc gcagatatca cggtgtttct
    70921 cgttggtcac caccatgtcc gcaggcagac ggtgagcaaa ggtgcccact tcacgcgcag
    70981 taccacacgc agaaggttgc ccggtcaggg agaacggacc gcaacccggc tgggaaattt
    71041 tgccggtcag caggtgcagg ttgtagacca ggttgttagc ccacacgcca cgagtatgct
    71101 ggttgaagcc catcgtccag taggagatga ctttcttgtt cggatcggca tacagctgcg
    71161 ccagttgttc taactggtct ttcggcacgc cggtcatttc ggcagttttt tccagcgtat
    71221 actcggcaac gaaggctttg taatcttcaa agctcatcgg ttcggaggcg tcagaacccg
    71281 gattcttcgc tgctttttcc agcggatggg tcggacgtaa accgtagccg atgtccgtcg
    71341 cccctttgcg caggttaacg tgcttgctga agaagtcctg atttatcgca ttgttttgaa
    71401 tgatatagtt ggcgatgtag ttcaggatca ccaggtcaga ttgcggcgta aagatgatgc
    71461 cgttatccgc cagctcgaag ctacgatgct ggtaggtaga aagcaccgcc acggtgacgt
    71521 tctggttaga gagacgacgg ttagtgatgc gcgaccagag gatcgggtgc atttccgcca
    71581 tgtttgcgcc ccacagcaca aacgcgtcag cctgctcgat gtcgtcatag cagcccatcg
    71641 gctcatccat accaaaggta cgcataaagc caactactgc cgacgccata cagtgacgcg
    71701 cgttcgggtc gatgttgttc gaacggaagc ccgctttaaa cagcttggac gcggcataac
    71761 cttcccagat agtccactga ccagaaccga acataccgat cgattccggc cctttttctt
    71821 tcagggcggt tttgaatttc tcttccatca catcgaaggc ctgatcccag gtgattgggg
    71881 taaattcgcc ttctttgtca tatttaccgt ttttcatacg cagcagcggc tgcgtcaaac
    71941 ggtctttacc gtacatgatt ttgggcagga aatagccctt aatgcagttc aggccacggt
    72001 taaccggtgc gtccgggtcg ccctgacagg ccaccacacg tccctgctgc gttccgacca
    72061 gaacgccgca accagtaccg cagaaacggc acggcgcttt atcccatttg atggcttcct
    72121 gctgaccaac aacggcgcgg gcaacgcccg gcacgctgag accggcagcc gccgcagcgg
    72181 ccgcaacggc gttagctttc ataaagctac gacgactgag tttcatggtg tttcctcacc
    72241 ttgctcttcc tgctggtgat aaaccagcga caccgccagc acgccctcta cgttgcgtac
    72301 tgactcaatg gtttggatca gcgtttcgct gtcttctgct tccaccacca caatcaactg
    72361 accgctcggc gcgtcgctga cagcaacttc acagccggga aaggcgttca gttgggtgct
    72421 gatgtctgaa attcgttcgc ttttggcctg cacgaccagg ctgcaaactt gccagttagt
    72481 gtgcatggag atactccgca gttatggctg ataccggaca gctggctgca catgctccac
    72541 atccgttgca aagttggcta ttaagttgcg gctggtagat cccggagagc gtcgggcgaa
    72601 agataattgc cattggctca cagctatcct gacagcggcg gcattcaact gactgatacg
    72661 ccaggcacgc gtccccgatg gtgaactgca aatcccaggc cctggtgtgg cgcggagaaa
    72721 atagcgattc ggggcaagcc tgcgcgcagt cgtaacagaa gctgcactcg ttatttttga
    72781 aattaacgct cggatagccg cccgcgccgc gttgcagaat gttgttttca caagcattaa
    72841 tacaggcgtc gcagcgggtg caatgggtca gaaaatgaga ttcatcaccg gaccagggcg
    72901 gacggatacc gttactggct ttgcgccagc gaccagtgag tatgccccga cgggatgcat
    72961 caatcttcac attgaccttc catcattaac gcgctctgaa aattgagagc gaccaaataa
    73021 accgcataat taataagcca tttttatagc cgctaagata ttaaaggatg tgtcaaagat
    73081 gcataccccg atcggggtaa aacctgtagc aggatcaaaa gagtggcggg aagcgtggca
    73141 aaaacgggct tttgctcaca tttcaaatgg ttataaatat atttatatag cgattaattc
    73201 accagagata ttt
//
LOCUS       DAZFZC010000012        67557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFZC010000012 DAZFZC010000000
VERSION     DAZFZC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.012"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(24..2213)
                     /gene="pdeA"
                     /locus_tag="ACHKXP_000933"
     CDS             complement(24..2213)
                     /gene="pdeA"
                     /locus_tag="ACHKXP_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO7645998.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2422..2497)
                     /locus_tag="ACHKXP_000934"
     tRNA            complement(2422..2497)
                     /locus_tag="ACHKXP_000934"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2462..2464),aa:Ala,seq:ggc)
     gene            complement(2537..2612)
                     /locus_tag="ACHKXP_000935"
     tRNA            complement(2537..2612)
                     /locus_tag="ACHKXP_000935"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2577..2579),aa:Ala,seq:ggc)
     gene            2833..3192
                     /gene="yfeC"
                     /locus_tag="ACHKXP_000936"
     CDS             2833..3192
                     /gene="yfeC"
                     /locus_tag="ACHKXP_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7645999.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3194..3586
                     /gene="yfeD"
                     /locus_tag="ACHKXP_000937"
     CDS             3194..3586
                     /gene="yfeD"
                     /locus_tag="ACHKXP_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7646000.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     VEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3638..5053)
                     /gene="gltX"
                     /locus_tag="ACHKXP_000938"
     CDS             complement(3638..5053)
                     /gene="gltX"
                     /locus_tag="ACHKXP_000938"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO7646001.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            5312..5387
                     /locus_tag="ACHKXP_000939"
     tRNA            5312..5387
                     /locus_tag="ACHKXP_000939"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5345..5347,aa:Val,seq:tac)
     gene            5432..5507
                     /locus_tag="ACHKXP_000940"
     tRNA            5432..5507
                     /locus_tag="ACHKXP_000940"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5465..5467,aa:Val,seq:tac)
     gene            5554..5629
                     /locus_tag="ACHKXP_000941"
     tRNA            5554..5629
                     /locus_tag="ACHKXP_000941"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5587..5589,aa:Val,seq:tac)
     gene            5634..5709
                     /locus_tag="ACHKXP_000942"
     tRNA            5634..5709
                     /locus_tag="ACHKXP_000942"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5667..5669,aa:Lys,seq:ttt)
     gene            5740..5865
                     /locus_tag="ACHKXP_000943"
     ncRNA           5740..5865
                     /ncRNA_class="other"
                     /locus_tag="ACHKXP_000943"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5974..6858)
                     /gene="xapR"
                     /locus_tag="ACHKXP_000944"
     CDS             complement(5974..6858)
                     /gene="xapR"
                     /locus_tag="ACHKXP_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO7646002.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARIEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVDVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPVMVKLLAALTQ"
     gene            complement(6899..7057)
                     /locus_tag="ACHKXP_000945"
     CDS             complement(6899..7057)
                     /locus_tag="ACHKXP_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO7646003.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(7110..8366)
                     /gene="xapB"
                     /locus_tag="ACHKXP_000946"
     CDS             complement(7110..8366)
                     /gene="xapB"
                     /locus_tag="ACHKXP_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO7646004.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWGGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVTH"
     gene            complement(8426..9259)
                     /gene="xapA"
                     /locus_tag="ACHKXP_000947"
     CDS             complement(8426..9259)
                     /gene="xapA"
                     /locus_tag="ACHKXP_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO7646005.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            9508..10272
                     /gene="yfeN"
                     /locus_tag="ACHKXP_000948"
     CDS             9508..10272
                     /gene="yfeN"
                     /locus_tag="ACHKXP_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO7646006.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(10311..11237)
                     /gene="yfeR"
                     /locus_tag="ACHKXP_000949"
     CDS             complement(10311..11237)
                     /gene="yfeR"
                     /locus_tag="ACHKXP_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7646007.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            11327..12325
                     /gene="yfeH"
                     /locus_tag="ACHKXP_000950"
     CDS             11327..12325
                     /gene="yfeH"
                     /locus_tag="ACHKXP_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO7646008.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(12322..12540)
                     /gene="ypeB"
                     /locus_tag="ACHKXP_000951"
     CDS             complement(12322..12540)
                     /gene="ypeB"
                     /locus_tag="ACHKXP_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO7646009.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(12542..14557)
                     /gene="ligA"
                     /locus_tag="ACHKXP_000952"
     CDS             complement(12542..14557)
                     /gene="ligA"
                     /locus_tag="ACHKXP_000952"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO7646010.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(14628..15614)
                     /gene="zipA"
                     /locus_tag="ACHKXP_000953"
     CDS             complement(14628..15614)
                     /gene="zipA"
                     /locus_tag="ACHKXP_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO7646011.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            15844..16605
                     /gene="cysZ"
                     /locus_tag="ACHKXP_000954"
     CDS             15844..16605
                     /gene="cysZ"
                     /locus_tag="ACHKXP_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO7646012.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            16790..17761
                     /gene="cysK"
                     /locus_tag="ACHKXP_000955"
     CDS             16790..17761
                     /gene="cysK"
                     /locus_tag="ACHKXP_000955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO7646013.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            18145..18402
                     /gene="ptsH"
                     /locus_tag="ACHKXP_000956"
     CDS             18145..18402
                     /gene="ptsH"
                     /locus_tag="ACHKXP_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO7646014.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            18447..20174
                     /gene="ptsI"
                     /locus_tag="ACHKXP_000957"
     CDS             18447..20174
                     /gene="ptsI"
                     /locus_tag="ACHKXP_000957"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO7646015.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            20215..20724
                     /gene="crr"
                     /locus_tag="ACHKXP_000958"
     CDS             20215..20724
                     /gene="crr"
                     /locus_tag="ACHKXP_000958"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO7646016.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(20767..21618)
                     /gene="pdxK"
                     /locus_tag="ACHKXP_000959"
                     /gene_synonym="thiJ"
     CDS             complement(20767..21618)
                     /gene="pdxK"
                     /locus_tag="ACHKXP_000959"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO7646017.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVVTSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            21723..22091
                     /gene="yfeK"
                     /locus_tag="ACHKXP_000960"
     CDS             21723..22091
                     /gene="yfeK"
                     /locus_tag="ACHKXP_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO7646018.1"
                     /translation="MKKIICLVITLLMTLPVYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCYEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(22094..23005)
                     /gene="cysM"
                     /locus_tag="ACHKXP_000961"
     CDS             complement(22094..23005)
                     /gene="cysM"
                     /locus_tag="ACHKXP_000961"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO7646019.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(23139..24236)
                     /gene="cysA"
                     /locus_tag="ACHKXP_000962"
     CDS             complement(23139..24236)
                     /gene="cysA"
                     /locus_tag="ACHKXP_000962"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO7646020.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMAALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(24226..25101)
                     /gene="cysW"
                     /locus_tag="ACHKXP_000963"
     CDS             complement(24226..25101)
                     /gene="cysW"
                     /locus_tag="ACHKXP_000963"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO7646021.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(25101..25934)
                     /gene="cysT"
                     /locus_tag="ACHKXP_000964"
     CDS             complement(25101..25934)
                     /gene="cysT"
                     /locus_tag="ACHKXP_000964"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO7646022.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(25934..26950)
                     /gene="cysP"
                     /locus_tag="ACHKXP_000965"
     CDS             complement(25934..26950)
                     /gene="cysP"
                     /locus_tag="ACHKXP_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO7646023.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(27108..27899)
                     /gene="ucpA"
                     /locus_tag="ACHKXP_000966"
     CDS             complement(27108..27899)
                     /gene="ucpA"
                     /locus_tag="ACHKXP_000966"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO7646024.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(28028..28885)
                     /gene="murR"
                     /locus_tag="ACHKXP_000967"
     CDS             complement(28028..28885)
                     /gene="murR"
                     /locus_tag="ACHKXP_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO7646025.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            29049..29945
                     /gene="murQ"
                     /locus_tag="ACHKXP_000968"
     CDS             29049..29945
                     /gene="murQ"
                     /locus_tag="ACHKXP_000968"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO7646026.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            29949..31373
                     /gene="murP"
                     /locus_tag="ACHKXP_000969"
     CDS             29949..31373
                     /gene="murP"
                     /locus_tag="ACHKXP_000969"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO7646027.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWVCARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            31378..32682
                     /gene="pbp4b"
                     /locus_tag="ACHKXP_000970"
     CDS             31378..32682
                     /gene="pbp4b"
                     /locus_tag="ACHKXP_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO7646028.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(32922..33821)
                     /gene="yfeX"
                     /locus_tag="ACHKXP_000971"
     CDS             complement(32922..33821)
                     /gene="yfeX"
                     /locus_tag="ACHKXP_000971"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO7646029.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLNQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(33917..34492)
                     /gene="yfeY"
                     /locus_tag="ACHKXP_000972"
     CDS             complement(33917..34492)
                     /gene="yfeY"
                     /locus_tag="ACHKXP_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO7646030.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(34553..35002)
                     /gene="yfeZ"
                     /locus_tag="ACHKXP_000973"
     CDS             complement(34553..35002)
                     /gene="yfeZ"
                     /locus_tag="ACHKXP_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO7646031.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(34989..35414)
                     /gene="ypeA"
                     /locus_tag="ACHKXP_000974"
     CDS             complement(34989..35414)
                     /gene="ypeA"
                     /locus_tag="ACHKXP_000974"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO7646032.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            35628..36497
                     /gene="amiA"
                     /locus_tag="ACHKXP_000975"
     CDS             35628..36497
                     /gene="amiA"
                     /locus_tag="ACHKXP_000975"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO7646033.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            36501..37400
                     /gene="hemF"
                     /locus_tag="ACHKXP_000976"
     CDS             36501..37400
                     /gene="hemF"
                     /locus_tag="ACHKXP_000976"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO7646034.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(37406..38458)
                     /gene="eutR"
                     /locus_tag="ACHKXP_000977"
     CDS             complement(37406..38458)
                     /gene="eutR"
                     /locus_tag="ACHKXP_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO7646035.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVPGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(38504..39004)
                     /gene="eutK"
                     /locus_tag="ACHKXP_000978"
     CDS             complement(38504..39004)
                     /gene="eutK"
                     /locus_tag="ACHKXP_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO7646036.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(39017..39676)
                     /gene="eutL"
                     /locus_tag="ACHKXP_000979"
     CDS             complement(39017..39676)
                     /gene="eutL"
                     /locus_tag="ACHKXP_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO7646037.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MIAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLATYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(39686..40573)
                     /gene="eutC"
                     /locus_tag="ACHKXP_000980"
     CDS             complement(39686..40573)
                     /gene="eutC"
                     /locus_tag="ACHKXP_000980"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO7646038.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(40594..41955)
                     /gene="eutB"
                     /locus_tag="ACHKXP_000981"
     CDS             complement(40594..41955)
                     /gene="eutB"
                     /locus_tag="ACHKXP_000981"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO7646039.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(41967..43370)
                     /gene="eutA"
                     /locus_tag="ACHKXP_000982"
     CDS             complement(41967..43370)
                     /gene="eutA"
                     /locus_tag="ACHKXP_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO7646040.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQTAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAALTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(43367..44593)
                     /gene="eutH"
                     /locus_tag="ACHKXP_000983"
     CDS             complement(43367..44593)
                     /gene="eutH"
                     /locus_tag="ACHKXP_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO7646041.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(44810..45997)
                     /gene="eutG"
                     /locus_tag="ACHKXP_000984"
     CDS             complement(44810..45997)
                     /gene="eutG"
                     /locus_tag="ACHKXP_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO7646042.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLTVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVTLLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(45987..46823)
                     /gene="eutJ"
                     /locus_tag="ACHKXP_000985"
     CDS             complement(45987..46823)
                     /gene="eutJ"
                     /locus_tag="ACHKXP_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO7646043.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(46834..48237)
                     /gene="eutE"
                     /locus_tag="ACHKXP_000986"
     CDS             complement(46834..48237)
                     /gene="eutE"
                     /locus_tag="ACHKXP_000986"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO7646044.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(48249..48536)
                     /gene="eutN"
                     /locus_tag="ACHKXP_000987"
     CDS             complement(48249..48536)
                     /gene="eutN"
                     /locus_tag="ACHKXP_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO7646045.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(48643..48936)
                     /gene="eutM"
                     /locus_tag="ACHKXP_000988"
     CDS             complement(48643..48936)
                     /gene="eutM"
                     /locus_tag="ACHKXP_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO7646046.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(48975..49991)
                     /gene="pta"
                     /locus_tag="ACHKXP_000989"
     CDS             complement(48975..49991)
                     /gene="pta"
                     /locus_tag="ACHKXP_000989"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7646047.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(49988..50791)
                     /gene="eutT"
                     /locus_tag="ACHKXP_000990"
     CDS             complement(49988..50791)
                     /gene="eutT"
                     /locus_tag="ACHKXP_000990"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO7646048.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(50788..51489)
                     /gene="eutQ"
                     /locus_tag="ACHKXP_000991"
     CDS             complement(50788..51489)
                     /gene="eutQ"
                     /locus_tag="ACHKXP_000991"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO7646049.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(51464..51943)
                     /gene="eutP"
                     /locus_tag="ACHKXP_000992"
     CDS             complement(51464..51943)
                     /gene="eutP"
                     /locus_tag="ACHKXP_000992"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO7646050.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFNHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(51956..52291)
                     /gene="eutS"
                     /locus_tag="ACHKXP_000993"
     CDS             complement(51956..52291)
                     /gene="eutS"
                     /locus_tag="ACHKXP_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO7646051.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(52584..54863)
                     /gene="maeB"
                     /locus_tag="ACHKXP_000994"
     CDS             complement(52584..54863)
                     /gene="maeB"
                     /locus_tag="ACHKXP_000994"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO7646052.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALAPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSSLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            55152..56102
                     /gene="tal"
                     /locus_tag="ACHKXP_000995"
     CDS             55152..56102
                     /gene="tal"
                     /locus_tag="ACHKXP_000995"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7646053.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            56122..58125
                     /gene="tkt"
                     /locus_tag="ACHKXP_000996"
     CDS             56122..58125
                     /gene="tkt"
                     /locus_tag="ACHKXP_000996"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7646054.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(58220..59263)
                     /gene="ypfG"
                     /locus_tag="ACHKXP_000997"
     CDS             complement(58220..59263)
                     /gene="ypfG"
                     /locus_tag="ACHKXP_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO7646055.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(59389..59964)
                     /gene="nudK"
                     /locus_tag="ACHKXP_000998"
     CDS             complement(59389..59964)
                     /gene="nudK"
                     /locus_tag="ACHKXP_000998"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO7646056.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIESCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(60032..62011)
                     /gene="aegA"
                     /locus_tag="ACHKXP_000999"
     CDS             complement(60032..62011)
                     /gene="aegA"
                     /locus_tag="ACHKXP_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO7646057.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            62217..63917
                     /gene="narQ"
                     /locus_tag="ACHKXP_001000"
     CDS             62217..63917
                     /gene="narQ"
                     /locus_tag="ACHKXP_001000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO7646058.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTNLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            64081..67194
                     /gene="acrD"
                     /locus_tag="ACHKXP_001001"
     CDS             64081..67194
                     /gene="acrD"
                     /locus_tag="ACHKXP_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO7646059.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(67293..67352)
                     /gene="ypfM"
                     /locus_tag="ACHKXP_001002"
     CDS             complement(67293..67352)
                     /gene="ypfM"
                     /locus_tag="ACHKXP_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO7646060.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        16067 a        18498 c        17138 g        15854 t
ORIGIN      
        1 attccccggt ttttttacgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
       61 ccttgcagat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
      121 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
      181 atcgcatcca gcgtgttcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
      241 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggtgccaaa gtcatcaatc
      301 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
      361 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
      421 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
      481 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
      541 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
      601 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
      661 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
      721 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
      781 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
      841 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
      901 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
      961 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
     1021 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
     1081 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
     1141 tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
     1201 cgaaagttgg gtaggagcgt taacggatct gtcagcgcct gcaaatgcca gcggcgatta
     1261 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
     1321 agcaccgcaa gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
     1381 ttgtaattca gaaggcaaag cgttgaaaca gcccaggtga gatttaaaaa cggatagcga
     1441 agcttaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
     1501 aagtcgtttt cataaggtgt gcacaacaga agtagcagca cgcttaacgc tgccagccag
     1561 cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct gcgccacaat
     1621 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
     1681 atcagcacag cggtgaaaag gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg
     1741 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
     1801 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
     1861 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
     1921 aacacgacaa acgtctggct accgagcatg accgcagtct gtaaaaaaga gagatgaaag
     1981 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
     2041 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
     2101 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
     2161 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
     2221 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
     2281 caaacagggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcac
     2341 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
     2401 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
     2461 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
     2521 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
     2581 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
     2641 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
     2701 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
     2761 tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
     2821 cagggaattg ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc
     2881 tatagccgcc agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa
     2941 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
     3001 attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
     3061 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
     3121 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
     3181 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
     3241 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
     3301 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
     3361 cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga
     3421 gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc
     3481 gatagataat atgacagacg ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg
     3541 aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag
     3601 gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag
     3661 caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa
     3721 cggtaacatc cagtgctgga gactgccccg caccggttac ggcgacacgc agcggcatac
     3781 caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt
     3841 tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac
     3901 gcgctaccgg acgcagatgt tttttcgcgg cgtcggcatc gaactcagca aaatcttcgt
     3961 agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt
     4021 tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc
     4081 actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc
     4141 acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt
     4201 atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca
     4261 ccagatagtt cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga
     4321 ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg
     4381 cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgt ggcgtgttgt
     4441 tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca
     4501 cacagaagtt ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct
     4561 ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg
     4621 ggttagcaaa acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc
     4681 agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca
     4741 ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca
     4801 ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc
     4861 tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat
     4921 cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt
     4981 aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag
     5041 ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg
     5101 aaaaattaag tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt
     5161 ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca
     5221 tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga
     5281 actctcccta taatgcgact ccacacagcg ggggtgatta gctcagctgg gagagcacct
     5341 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaact actttatgta
     5401 gtctccgccg tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct
     5461 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccactt tctcgccagc
     5521 taaatttctt gtaaaaatgt gaagtaccga agtgggtgat tagctcagct gggagagcac
     5581 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac ttcgggtcgt
     5641 tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac
     5701 gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg atactcaaag
     5761 attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg ctcgcggcga
     5821 cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgta aaaaagcgcc
     5881 ctaaaggcgc ttttttgcta tctgcgattt gcgaaattgc ctgatgcgct tcacttagca
     5941 gactactatt tccggcaatt cctgtctcct cacctactgt gtcaatgcag ccaacagctt
     6001 aaccatcacg ggcgtcacct gctgtgtttc ataaacaata tataaatctg cagggatgcg
     6061 ctgtttgagc ggacggaaaa tgacacctgg ccagttcatt tgtgcgtagc tgtccgctat
     6121 caatgtgata ccaatgccca tactgaccat agcgagtacc gtttgcggtt cattaacttc
     6181 gcgaataaca accggtgaaa atcccacctg ctggcaaact cgctgcaaaa aatcccagtc
     6241 agtgtaaacg ggcggcattg taacaaaata ctcgtcacgt agcgcttcca gcgggacggt
     6301 ggaaaatgat gagagatgat gctcttcagg catcgccacc agaaacgccg attcatgcaa
     6361 ccgtaagctg gtaaaaccag tcggtggttc tgtcgccatt cgccagatcc cggcatcaag
     6421 ttcgcggcgt tccagcaagg ccatttgcat cgcgggcatc ttttcgcgaa aaagaacgtc
     6481 aacgttagga ttttccctga ggaatcgccg cataaccggg cgcatccgtc cccacattgc
     6541 cgttcccact acgccgagtt caatccgccc tgcttctccc cgacctattt gttcaatccg
     6601 agccaataca ttattagcat tcaccagcaa tcgacgcgat tcttccatca agattttgcc
     6661 cgcgtgtgtc agtacgacgc tgcgcgaatg gcgaataaaa agctgcgtgc cgagttgatt
     6721 ttccagctct ttaatatgaa tgctgagcgg aggctgagac atatttaaac gcgctgctgc
     6781 gcggccaaaa tgcaactctt ccgctacggc aagaaaataa cggagcaact taagatctgt
     6841 tctgtatacg cgttccataa ttaagtgctc catataataa atacatcgta ttaaattatc
     6901 atggcataaa gtatttcacc accagatatc cgacatacat aaacacaatg gataatatac
     6961 ccggtaatac atttggtaca ggaataggca agcgcacgat tgtaaacaaa gcaccaatac
     7021 caaaaccaac gccggaagaa aataaaatat ctttcactaa ttaaccttta tcataaaagc
     7081 agctctgaag agcagagccg cgaatccttt taatgagtca ccgctcgatg ctttatcttt
     7141 tcagggtcat gattatattt aaacccaaag aaaaatatca ctgcgagaaa aagagcatat
     7201 cctgcaaaca ccagccagat agtttgccag tcttttacgc catccaccga aaagtaatct
     7261 actgccatgc cactcagaat cgagccaccc catgcgccga caccatttac catggtcata
     7321 aagagcccct gcgcgctggc acgaatgctg gaatcaactt cctgttcgac aaataccgaa
     7381 ccagaaatat tgaagaaatc gaatgcacag ccataaacaa tcatcgacag cagcagcaaa
     7441 ataaatccgg ttgttgacgg atcgccatag gcgaagaagc caaagcgcag cgtccaggcc
     7501 accatactca tcagcatgac ggttttaatg ccaaatcgct ttaaaaagaa tgggatagtc
     7561 agtataaagc ccacttctgc catctgtgaa actgacagta aaatggaggg atatttcacc
     7621 acaaaactgt cagcaaactc cgggttacgg gcgaaatcat gtaggaacgg attaccaaaa
     7681 acgttggtaa tttgcagtac cgcacccagc atcatggcaa agaggaaaaa gatggccatg
     7741 cgtggatttt taaacagcac gaaggcatcc agacccagct tgctggcaag cgatgtggtc
     7801 gcttttttct ccgcaaccgg aatcttcggc aaagtcagcg cataagccga cagcagcaat
     7861 gacgcaccgg acgcgatata cagctgcaga ctactcaatt ccagatgcag caggcttact
     7921 gcccacatcg cgacaatgaa ccccaccgta ccaaaaacgc gaatgggcgg gaaagcggtc
     7981 accgggtcaa gccctgcctg ggcaagacag gaataagaga cgctgttcga taacgcaata
     8041 gtcggcataa acgccatcgc attgactaac atcacccaaa acatcatatc cggatcagtt
     8101 acggatgccg cataaaaaag tacgcccgca cacaccaggt gacacagcat gtatgcacgt
     8161 tctgcgcgca gccatttgtc tgcgatgatc cccattatac caggcataat aatcgcggcg
     8221 atccctttgg aactgtaaac catgccaaca ttagcgccgg tgaaatgaag agtattaatc
     8281 atgtaagagc cgagggtaac cagccagctc ccccagataa aatattgcaa aaaggacatt
     8341 acctttaagc gcatcgcgat gctcataatc gtttccttgt cgcatcattt ggcctcaccc
     8401 gcgaggccgt ttcttattgt ttttctcagg caattttgcg cagaaagcca caaataaggt
     8461 tgatgaagtt ctgcttagag agctccgcag ccgccagcgt ttgcgcatgt gacaatttca
     8521 catcgcttaa accttccgcc atattggtaa tcgcagagac cgcaacgact ttaaggtcgc
     8581 aatggcgagc tgaaataacc tcaggcacca cagacatacc aacaacatcc ccaccaataa
     8641 tttgcatcat gcgaatttcc gccgcagtct cgaaattcgg ccccggatac gagacgaaca
     8701 cgccctccgt cagagggaaa ccctcttctt tcgccacttt ttgtaacagt gcgcggtatt
     8761 ccgcatcgta ggcatttgcc agtgagaaga agcgctctcc aaaacgatca tcgttaagac
     8821 ccaccatcgg cgtaccgggc atggtgttga tatgatcttt caatgcgacc aggctgcctg
     8881 cccccacttc cggacgcagc gagcctgccg cattggtgca gaacagtagc tcgcagccca
     8941 gcagcttaaa ggtacgaatt gcgtcagtca taatggtcat tccacgacct tcgtagaaat
     9001 gtccgcgacc tttcatacat accaccggta cgccctggag atggcccagc actaactccc
     9061 ccgcatgacc atgtaccgta cttaccggga agcccggcag cttttcgtag gaaattgcga
     9121 cagcgttctc aatctgatcg gccagcgcgc cgagcccgga acctaaaata aaggccactc
     9181 gtggcgtgaa atcaggttta taagtcttga taatatctat gcaaaacagc gggttatgag
     9241 aaaattgaac ctgagacata catatccttt ttctgtaggg tggaatctaa cgccttttta
     9301 tagcgaggtt gtcccggctc ttaccaatac acttttctca cgcattcaat agcgattcga
     9361 tatcgatgga tttatatctt ttctctatta atccacggta aaaactgtat tggcgatatt
     9421 aattcctttg cattaacaat atgtcgtttt tagcaaacca ctttattaag caatctacta
     9481 ataacgatcg gcatagggaa ttatgctatg aaaaaacatc ttttaactct gacactttcc
     9541 tctatattag cgatacctgt tgtatctcat gcagaattta aaggcggttt tgcagatatc
     9601 ggcgtgcatt atctggactg gaccagccgg actaccgaaa aatcgtcgac caaatcacac
     9661 aaagatgatt ttggttatct tgaatttgaa ggtggcgcta actttagttg gggagaaatg
     9721 tacgggttct tcgactggga aaacttctat aacggtcgcc ataataaacc aggtagcgag
     9781 cagcgttata cctttaaaaa taccaaccgt atttacctgg gtgacaccgg atttaatctc
     9841 tacttgcatg cgtatggcac ctacggttct gcgaatcgcg tgaatttcca cgacgatatg
     9901 ttcctgtacg gtatcggcta caatttcacc ggcagcggtt ggtggttcaa accgttcttt
     9961 gctaagcgtt atacagatca aacctattac actggcgaca acggctatgt cgccggctgg
    10021 gttgcaggtt acaactttat gctgggcagc gagaaattca ctcttaccaa ctggaacgag
    10081 tacgagtttg accgtgacgc tacctatgca gcgggtaatg gcggtaaaga agggctgaat
    10141 ggcgcagttg cactgtggtg gaatgcaaca tcacacatta ctacggggat tcagtatcga
    10201 tatgcggatg acaaactggg cgaagatttt taccaggatg cgataatcta ctccatcaaa
    10261 tttaacttct gattgattac tgccggggct cccggcagta tgaattcact ctatatctga
    10321 tacaacggat ccccttcccg cccagccatt agcgcattgc cctggtctcg taccacgtcc
    10381 cataaagctt cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg
    10441 cgttcaacaa ccggcgtaat gcgctttacc accagtgggc taccttcagg caatggtagc
    10501 gccagtgcgg gaagaatact gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc
    10561 ggatgtccaa tctcctgcac aatattcgcc tgaataccat tgcgcgccag cgctgcgtca
    10621 atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc
    10681 tgccagggta cataatcttc cacggccaaa gcgctatcgc gatggcaaag cagaaagaaa
    10741 ggctcggaaa gaatcgcttc acattgcaga tcgcccactg ggcctggatc gatgacgatg
    10801 ccaaaatcga catctccctg gcgaatactt tccattaccc actgctgcgg acgatcgtgc
    10861 aggacaaact gaatatctgg atagcggcga tggctttcag caatgcattg cggaataaga
    10921 tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc cgctcagttg ttgccccata
    10981 cgcccggtat cgcgcaacgt gctgttcagt tcatccagca gtcgctcaag acgcaaagcc
    11041 agctgctgcc ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag caggcgaaca
    11101 ccagtatgat tttccagttc cttcacactg tgactcactg ccgactggct caggccgata
    11161 cgctctcctg cacgactaaa acttttctcc tgcgctactg tgacgaaaac ctttaattgt
    11221 tttaaagaat aattcatctg ttttcttcat gaatggatgc aataaatcaa ttttatttct
    11281 caaactggaa gaagcacaat agaaccatcg atcatctgga gtctttatga aactttttcg
    11341 tatcctcgat cctttcacct taaccctgat cacggtggtg ttgctggcct ctttctttcc
    11401 ggccagaggc gatttcgtcc ccttctttga aaatctgacc accgcagcta ttgccctgct
    11461 gttctttatg cacggcgcga agttgtcgcg tgaggcgatt attgctggcg gtggtcactg
    11521 gcgactgcat ttgtgggtaa tgtgcagcac cttcgtgctg tttccgattc tgggtgtact
    11581 gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc tactccggtt ttctctactt
    11641 gtgcattctc ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa
    11701 cgtcgcggca gcggtttgtt ctgcgtcggc atccagcctg ctggggattt tcctttcacc
    11761 attgctggtt ggtctggtga tgaatgttca cggtgcaggg ggcagccttg agcaggtcgg
    11821 taaaattatg ctgcaactgc tgctgccgtt tgtgttgggg catctttccc ggccgtggat
    11881 tggtgactgg gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga cgtccattct
    11941 gttggtggtt tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg
    12001 ctggggatca ttgctgttta tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt
    12061 agttaacgtc tttatggcac gccgactgag cttcaataag gcagatgaaa ttactatcgt
    12121 cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca ttctgttccc
    12181 cacatcggtg atcggtatga tggtgctgcc cctgatgatt ttccatcaga tccaattgat
    12241 ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc gaacagttac aggcgcagca
    12301 ggaaagcagc gccgataaag cttaaagcgg acgcttcagg ggctggacca gttgcgtcag
    12361 cccctcggtt ttaatcagca gcgtgatttg cattagctca ccgagcttcc ctgccgggaa
    12421 ctcatctttg cgggcaaacc acagcagata ctcctccggc aggtcgatta agcgacgccc
    12481 tttgtatttg ccaaacggca ttatcgtatt ggctatttca atcagctgct ctttttccat
    12541 ctcagctacc cagcaaacgc agcatttccg cttcgtcgat gacttcaatg cccagttcct
    12601 gcgccttcgc cagtttagat cctgcagctt cacccgctat caccagatcg gttttcttcg
    12661 acacgctgcc cgcgactttc gcgcccagtt cgaccagtcg agctttagcg tcatcacgcg
    12721 acatctggct taagctgccc gtaagcacca cggttttacc agcaaacggg ctgtcaatct
    12781 cttccgcgtt gataacgatc ggcgcaggcc agtgaacacc ttccgccaac agctcgctga
    12841 tgacattgcg gttgctttct tcggcaaaga agttgtgaac gtgggatgca acgacaatgc
    12901 caacatcagg caccttttgc agctcttcaa tcgaagcggc ttccagcgct tccagcgtgc
    12961 cgaaatatgc cgccagacct gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat
    13021 agaggaagcg agcaaaggtg gtttctttcg ctttttccag cgcgttaacc acgttttgtg
    13081 ccgattttgg ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat
    13141 ctgccggagt atggacatat tctttttcaa ccagctgatc gatgattttg tcgcccattc
    13201 cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag cgactcttta cgctgcgcac
    13261 cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag
    13321 aaccacatac cggacaatgc gtcgggaata caacctcacg ggtatcttcc gggcgttcag
    13381 aaagcacgac gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc accactttat
    13441 cgccaatgcg taaaccaaga cgttcgattt catccgcatt gtgtaaggtt gcgttactca
    13501 ccagcacgcc tgcaacatgg acaggttcca gacgcgcaac aggcgtaatc gcgccagtac
    13561 gcccaacctg aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa
    13621 acgctaccgc ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc tgtgccagtg
    13681 agttgacctt aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca
    13741 ctttgtgata gaacgccagc acttcttccg ccgattcaca aagcgttacc cgatcgctga
    13801 ccggcaaccc ccacttttta aattgcagta aacggccaag atgagtatcc ggcagctcgc
    13861 caccttccag aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac
    13921 gcggatcaag ctgacgcagt gaaccagctg ccgcattacg tgggttagca aaaactttcc
    13981 cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc aggaacactt
    14041 caccacgcac ttccagacgc gccgggatat tctctccgtg tagcttcagt ggaatggcgc
    14101 gaatggtacg cacgttagac gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg
    14161 cactgactaa aacgccattt tcatacagaa tactgacagc aaggccatcc agcttcagct
    14221 cacagcacca ggtgactttc tcgttgcttt tcagacggtc ctgcactcgt ttgttgaagg
    14281 caagaaagct ttcttcatca aaaacgttat ccagcgacag cattggcact tcatggcgaa
    14341 tctggctgaa agccgccagc ggcgcagcac ctacacgttg ggtaggcgaa tcaggcgtaa
    14401 tcagttctgg atgtttggtt tccagctcgc gcagttcgcg catcagcctg tcgtattcag
    14461 cgtcgggaat ttccggcgca tccatcacat gataaagata ttcatgatgg cgaagcgtcg
    14521 ttcgcagttc tgtcagttgt tgttcgattg attccatatc gcaccatcaa tgctaaaaac
    14581 ccccgacaag cgggggttcg aagaggagtt aatttgcctt aagtgtatca ggcgttggcg
    14641 tctttgactt cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt catcatacgg
    14701 cgctgatcgt caagcacgac accgcccact tcatcggcaa tatgctgcgc agattgcagc
    14761 atcagcttga agttctgcag ctcgtcaccg taagacggta cctgcataaa gatagtgaca
    14821 cccggagtag tgaaatcctt catttcagga tcaaaggttc ccggtttcac catattcgcc
    14881 aggctgaata acgccgggcc gctgccatcc gggctaagat gacgatggta aatattcata
    14941 tcgccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg
    15001 ctaccgtgat gcgccgcgac gttcatgata atcaccgctt ctttgcgctt cggtttatcc
    15061 ataactggag ccggttccgc tacaggctca ggctgtggtg ccgctacggg ttctgcaggc
    15121 tggaaagcct gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc
    15181 tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg gctgataggc aggctgctgc
    15241 accggctgcg ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg
    15301 accggttgac gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga
    15361 cgagcagcct catgctcctg agcgttagcc ggggcatgat tcacgcggtg aacacgaacc
    15421 tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac
    15481 ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag
    15541 aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc
    15601 aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta
    15661 ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac
    15721 aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc
    15781 gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg
    15841 attatggttt catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc
    15901 tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat
    15961 attttgttga tggggggcgc attctggtgg ctctttacgc aactcgatgt ctggatcccg
    16021 actctcatga gttatgttcc ggactggtta caatggctga gctatctgtt gtggcccctg
    16081 gctgtcatct ctgtgctgtt agtgtttggc tatttcttct caacgattgc taactggatt
    16141 gccgctccgt tcaacggttt attggctgaa caactggaag cacgattgac tggcgctaca
    16201 ccgccagata ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa
    16261 tggcaaaaat ttgcctggta tctgccacgc gcaattgtat tgctaattct ttacttcatc
    16321 cctggtattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta
    16381 gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc
    16441 accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagtctgttt
    16501 acgatgatcc cgctgcttaa tctgttcatt atgcccgttg ccgtttgtgg cgcgacggcg
    16561 atgtgggtcg attgctatcg cgataaacac gcaatgtggc ggtaacaatc taacggttat
    16621 tttgtaaacc gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat
    16681 gtcattattt cccttctgca tatagatatg ctaaatcctt acttccgcat attctctgag
    16741 cgggtatgct acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat
    16801 ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg
    16861 taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg
    16921 tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga
    16981 actggttgaa ccgaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg
    17041 cggttacaaa ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct
    17101 gaaagcgtta ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat
    17161 ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt
    17221 cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaga tctgggaaga
    17281 taccgacggt caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg
    17341 cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatatctg tcgccgttga
    17401 gccaaccgat tctccagtta tcgcccaggc gctggcaggt gaagagatta aacctggccc
    17461 gcataaaatt cagggtattg gcgctggttt tatcccggct aacctcgatc tcaagctggt
    17521 cgataaagtc attggcatca ccaatgaaga agccatttct accgcccgtc gtctaatgga
    17581 agaagaaggt attcttgcag gtatctcttc tggagcagct gttgccgcgg cgttgaaact
    17641 acaagaagat gaaagcttta ccaacaagaa tattgtggtt attctaccat catcgggtga
    17701 gcgttattta agcaccgcat tgtttgccga tctcttcact gagaaagaat tgcaacagta
    17761 atgccagctt gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc
    17821 ttcaaacttt tgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac
    17881 taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta
    17941 tgatttggtt caattcttcc tttagcggca taatgtttaa tgacgtacga aacgtcagcg
    18001 gtcaacaccc gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac
    18061 tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact
    18121 aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac
    18181 ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa
    18241 attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact
    18301 ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa
    18361 gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa
    18421 aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg
    18481 ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt
    18541 ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag
    18601 cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca
    18661 tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc
    18721 tgattaaaga taagcacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg
    18781 cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg
    18841 atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca
    18901 ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga
    18961 acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta
    19021 tcatggcgcg ttctctggaa ctacctgcta tcgtgggtac cggtagcgtc acctctcagg
    19081 tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa
    19141 ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag
    19201 agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtat
    19261 gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg
    19321 ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcactgccc actgaagaag
    19381 aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaagcg gttatcgttc
    19441 gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga
    19501 acccgttcct cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg
    19561 atcagctccg cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga
    19621 tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg
    19681 aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa
    19741 caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg
    19801 gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac
    19861 acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt
    19921 ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta
    19981 cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc
    20041 gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg
    20101 ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa
    20161 aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt
    20221 ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag
    20281 atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt
    20341 gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg
    20401 ccagtagacg gcaccattgg taaaatcttt gaaaccaacc acgcattctc tatcgaatct
    20461 gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa
    20521 ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa
    20581 tttgatctgc cgctgctgga agagaaagcc aagtctaccc tgactccggt tgttatctcc
    20641 aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc
    20701 ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc
    20761 atttttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat gctgctgggt
    20821 gtagcgcatc acttccagta cgcgcaaccc cgctcggtgc actgcatcgg ttaacgcctt
    20881 ccctttcagc aagccactga tgagctgagc acaaaacagg tcgccagtcc ctttcaggtc
    20941 ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcagtgacca ccacaacctg
    21001 catctcctga ttttcttcat taccggaggc gctggtaacc accacccatt ttaatgtgtc
    21061 tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat ttttaccggt
    21121 caggatttcc aactcaaaga tattgggggt aattccctgc gccagcggca gtaaatattg
    21181 tcgatacgct tcgggaaggt caggtttgac ataaattccg ctatcaatat cgccaatcac
    21241 cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca gccactcggc
    21301 aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac gaagttggcg
    21361 cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt ccggaatcgc
    21421 accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg gcacggcaaa
    21481 gacattcagg ccgttctgtt tgatagcagg cacggcaatg ctgttgccca cgctgccgta
    21541 aaccacctgc gactgcacgg cgacgatatc cgcctgcagt gccctactct tatcgttaaa
    21601 caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc gtaatccaac
    21661 cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga taaggtaatt
    21721 caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc gtttacgcga
    21781 agttaactgc ccatgaagaa gcccgcatca acgccatgct ggagggatta gcacagaaaa
    21841 aggatttgat atttgtgcgc aacggtgatg aacatacctg ctatgaagcg gtttctcatc
    21901 tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag tttattgata
    21961 aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc cccggtaaga
    22021 gcgatgagaa cgcacagcct tttttacatg cgctaattgc gcagacggat aaaacggtgc
    22081 ctgcgcaata atcttaaatc cccgccccct ggctaaaatg ctcttcccca aacaccccgg
    22141 tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca ccagggttag
    22201 cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca cagaatattc
    22261 cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga atatccagca
    22321 cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa
    22381 tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact ggtttggatt
    22441 gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg ctggagacaa
    22501 aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg gtgtaatggg
    22561 cgtaagggtt atcgggatta ttaaactgat cgagcagctt tccttcgcca cgattcgcca
    22621 tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca agaatcagtt
    22681 ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg ggcatcagca
    22741 atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg gtgttaccac
    22801 tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc gcctcgacga
    22861 tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct tccagtttta
    22921 accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc
    22981 ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc ctgatgcgac
    23041 gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag cccggatcgc
    23101 gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca
    23161 agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc atgttgcaga
    23221 ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc
    23281 ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg
    23341 gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct gatatccact
    23401 tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta gcccagcggc
    23461 cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag gcggttcact
    23521 tcgcccataa attcgagtac aaaacgggtc gctggttcgc gccatacctg attcggcgca
    23581 tcagcctgtt caatattccc ctgactcatc accactacac gatcggctac ttcggtcgcc
    23641 tcttcctggt cgtgggtcac aaacacgctg gtgaatttca gctcttcatg cagttgacgc
    23701 agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc
    23761 agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacacgctg tttctggccg
    23821 ccggaaagct gcgccggata acgatccgcc agatgcgcaa gctggaccat ttccagcaat
    23881 tttgtcactt tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc
    23941 aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta atgctggaac
    24001 acgaaaccga ctttacgatc gcgcgcgtgc aggcggctca cgtcggtgcc gtggaagcga
    24061 atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt ggttttcccg
    24121 gaaccggacg gccccagcaa cgcggccatc tgacctgaag gaatatccag tgagatatcg
    24181 ttcagcacct gggtgcgacc aaaagacttc ttaatattgg caatctcaat gctcatgatg
    24241 ttcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc cactgcaaca tactctttaa
    24301 aaacagggtg ataatcgcca tcagcgtcaa cagcgcggcg gcagtaaagg agccgacggt
    24361 gttgtagtcc tgctccagca attcaatctg taacggcagc gaaagtgttt cgccgcgaat
    24421 cgagccggaa accaccgaca ccgcgccaaa ctcgccaatc gcgcgggcgt tggtcagcac
    24481 tacgccataa agcagcgccc agcggatgtt cggcaatgtg acacgacgga acatctgcca
    24541 gccagacgcg ccaagcaaaa tcgccgcctc gtcttcctga ctgccctggc ttaacatcac
    24601 cggcaccagt tcgcgcacca caaacggaca cgtcacgaag atggtgacca gcaccattcc
    24661 cggccaggag aacataattt gcagattatg ttcatccagc caaccgccca gcgggccgtt
    24721 agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg caaacggaat
    24781 gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca ccagccaggc
    24841 cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag
    24901 ccagatggcg tgcagcatat ccggatcggc cagattctgt aaaaccggca tcagcccttt
    24961 gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg ccgaaaccag
    25021 catcccgatg ccaattagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt
    25081 caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga
    25141 gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc aatcgcgctc
    25201 gccgccgggt aatcaaactc ctgcaagcgc acaaaaatca tcagcgacgt cacttccgtc
    25261 ttccaggcga tgttcccggc gataaatatc accgcgccaa actcaccaag actacgggta
    25321 aacgacaacg ccacacccgc caccagcgcc ggagaaagtt ccggcagcac cactttgcag
    25381 aaactctgcc agcgcgttgc accaagcgtt tccgccgctt cttcatattc cgggcctaac
    25441 tcttccagca ccggctgcac ggtacgcacc acaaacggaa tgctggtaaa ggccatcgcc
    25501 accgcaatcc caagccaggt gtaggtgact ttgatatcaa acttcgccag ccattcaccg
    25561 taaaaaccgt tcacggaaaa gagcgaggcc agcgttaaac cggcgactgc cgttggcagc
    25621 gcaaagggta aatccatcag cgcatcaagc agcgtgcggc cagggaagcg ataacgagtt
    25681 aagatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac
    25741 agcagcgtca ctttgtaggc tgcgaccacc tgcgggttgg tgatcacctc ccagtactgc
    25801 gcccagctca tctgggccag ctgcatcacc agcgcggaga gtggcaggag caaaatcagg
    25861 caaacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac gcgtcttgat
    25921 gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg
    25981 tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg cggaacagct
    26041 cggtctgcgg gaatttgttt ttcagtttgt ccatgacttc cgggttattt acgcggtagt
    26101 aatagtcggt gatgatggtc tgcgcctgcg ggctgtagag ccagttcagg taggcttttg
    26161 ctgctttttc cgtaccgttg gcctgcacgt ttttatcgac ccacgccacc gggaattccg
    26221 ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcatac tgtttgcgga
    26281 tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc tcggcaaaag
    26341 tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg aactgggtca
    26401 taaactgttc ggttttggct ttgtcgccac cgtcagcttt atccgctgcg ccccatgccg
    26461 ccagataggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt
    26521 cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta cgcaccagga
    26581 agcccatggt ggagtagaac ggcgagctat tattcggcag gcgcgactgc cagtccgccg
    26641 ggataagctt gcctttatcg tgcagaattt gtacgtcggt cacctggtta taagtgacaa
    26701 cgtcggcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg gcatgagatt
    26761 gttttatcgt cagtttgtcg ccgccgttat ctttggccca ttgctgttca aacggcgggt
    26821 tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt tccgttgcct
    26881 gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt ttcagtaagt
    26941 taacggccat tgcgcaccct tataaattta atgactttct aatagccatc atatttataa
    27001 cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt tgaaaaggga
    27061 ataagacgaa atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg
    27121 ctgaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag
    27181 ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccaacgg atcggcgagg
    27241 cgacgcatcg ggattgcttt cgccatttca gtcagcaccg attctggatc ttccgggttc
    27301 gactggcggg caatgctttc cgccattggt gtgcgaacgt atccagggca tatggcgtta
    27361 acgcgaatac cagactgcgc atactccacc gccagcgatt ttgtcaggcc aacaatcgcc
    27421 gctttcgtta aggcgtacgc cgtttcaccc ggatcggcca ccatatcgcc agtgaccgaa
    27481 gacatcatca caatgcgacc atctttgcgg gcaatcatct ccggcagcac cgccttcgtg
    27541 acgttccata cgcctttaat attgatgtca atatggaaat cgcgatcgtc atcgctcata
    27601 tcgaggaaac tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct
    27661 tctttttcct tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg
    27721 acaaccgccg tacagcgatg accacgacca cacagttcgt ccgccagctt ttcgatctca
    27781 ggggagatat ccagcaagat taggtttgcg ccatgacgcg caaaagttct ggcaattcct
    27841 tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt
    27901 ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata
    27961 aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct
    28021 ttatgcatca tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgact
    28081 ccacatcatt gagttgcaca agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg
    28141 tgcgggtaga catcgacgag ctacgccatt cggtttcacc ggaaacggta tcgagcgtga
    28201 aatgcgccag ccgccgcagc ggagaatcag ccagagaagt aatggcaatc accgttgccc
    28261 cctgctttcg tgccgcttct gcgcataaga caatctcttt tttactgccg ctgtaagaga
    28321 tagcgatttg tacatcgcct tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt
    28381 cggcttcgca ggccacgcga tagccaattt tcatcagttt gaacgacaga tcgcgcccca
    28441 ccagcgccga tccgcccagg cccgttatct ggataaacgg tgctttactg atgacctcaa
    28501 tgattttttg cagccgcgcg tagtcgaaca acgcacaggt ttgctccagc gccagctctt
    28561 tttcgcgatt caatttgcgg gcaatcactt ccagcgaatc atcactggta atcgaactgt
    28621 gcaggtgcag cgccgtggca ttcgttttct cccggctggc actgtattcg cctattaacg
    28681 ccatacgcaa ttcagtaaat ccttgcgcgc cgagtttttg ggcaaatttc actatgctcg
    28741 actggctaat gccgagctgt ttcgccatct gccgtgaaga aacggattgc agctcactca
    28801 cgttggcctg cagaaagtca gcgatttttt gctcgttttc ggtgaattca ctccccgcat
    28861 tgcttatttt cgtcaggtac aacatcttcc actctcctgg gctggcagca taaattccgc
    28921 ttaagttctt atgacgctct tcacactctg cgagtgtaga ctcaatgatt cctttaagtc
    28981 tgtcaattcg ggatatatga ttccatacat ctgcaattat ttgatcgtaa atagtaaggt
    29041 cactaccgat gcaactagaa aagatgatta ctgaaggctc gaacaccgcc tcggctgaaa
    29101 ttgaccgcgt atcgacgctg gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac
    29161 cgcttgccgt tgagcgcgtc ctgccggata tcgccgcggc gatcgatgtt atccacgccc
    29221 aggtcagcgg cggcggtcgt ctgatttatc tcggtgcggg aacatccggt cgtctgggga
    29281 ttctggatgc cagcgaatgt ccgcccacct acggcgtgaa accgggtctg gtggttggtt
    29341 tgattgctgg aggcgaatat gccattcagc acgcggtgga aggcgctgaa gatagccggg
    29401 aaggtggtgt taacgatctg aaaaatatta atttaacggc acaggatgtg gtggttggca
    29461 ttgctgccag cggtcgcacg ccgtatgtga ttgccggact ggaatacgca cgccagctcg
    29521 gctgccgcac agtgggaatt tcctgtaatc cggggagtgc cgtttccacc accgccgagt
    29581 ttgccattac accgattgta ggtgccgaag tggtcactgg ttcttcgcga atgaaagcag
    29641 gcacagcgca gaaactggtg ctcaatatgc tttccaccgg gctgatgatt aaatctggca
    29701 aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc
    29761 aggtcaatat cgttaaaaac gccaccggat gtaatgccga acaagcggaa gcggcgttaa
    29821 ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg
    29881 cagaagctaa aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt ttagacaagg
    29941 aataacccat ggccaaagag atcagcagtg aacttctgaa caccatcctt acccgtgtcg
    30001 gcggaccggg aaatatcgcc agttgtggta actgtatgac gcgcctacgt ctgggtgtac
    30061 atgacagttc actggttgat cccaatatca aaacgctgga aggcgtgaag ggcgtcattt
    30121 tgaccagcga ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag
    30181 cgatgagcga gctgctgggc gaggcaccgg tacaggatgc cgcagaaatc gccgcccaga
    30241 acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca acaatttctc gccaaattcg
    30301 ccaccatctt cacgccgctg atccccggtt ttattgccgc cggtctgctg ctggggatag
    30361 cgacgttaat tgccacggtg atgcacgttc cggcagatgc tcagggcacg ctacccgacg
    30421 cgctgaattt tatgaaggtg ttcagcaaag gtttgttcac tttcctggtg attctggtgg
    30481 gctataacgc cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt attgccgcac
    30541 tctttttgct cggttataac cccgctgcaa ccaccggtta ctacgccggt tttcacgatt
    30601 tctttggtct gcccatcgat ccgcgcggca atattatcgg cgtgctgatt gccgcgtggg
    30661 tctgcgcccg cattgaaggc atggtgcgcc gctttatgcc ggacgatctc gacatgctgc
    30721 tgacctcgtt aatcaccctg ctgatcaccg ctacgcttgc gtacctgatc attatgccgc
    30781 tgggtggctg gttattcgaa ggtatgtcat ggctgtttat gcacctgaac agtaatccgt
    30841 tcggttgtgc ggttttagcc gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg
    30901 gctttattcc tgtttacctc gcgttaatgg acagccaggg attcaacagc ttatttccca
    30961 tcctttcaat ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc
    31021 aaccgcacag tgcattacgc agtcaggtac gcggggcgat tattcccggt ctgctgggcg
    31081 ttggtgaacc gttgatttac ggcgtcaccc tgccccgcat gaaaccgttt attaccgcct
    31141 gtttaggcgg cgcagcgggc ggtttgttta tcggcctgat agcctggtgg ggtctgccga
    31201 tgggcttaaa cagcgccttt gggccgtctg gtctggtggc gctgccgctg atgacttccg
    31261 cacaaggcat ccttccggca atggcggttt atgctggcgg gattctggtg gcatgggttt
    31321 gcgggtttat tttcaccacg ctctttggct gccgtaacgt caatctggac tgatatcatg
    31381 aaacggacaa tgctctatct ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa
    31441 taccctgttc tgacagaaag ttcgccagag aaagcagggt tcaacgtcga acggcttaac
    31501 cagatggatc gctggattag ccagcaagtg gacgccggtt atcccggcgt aaacctgctg
    31561 atcattaaag ataatcagat tgtgtatcgc aaagcctggg gagcggcgaa aaagtacgat
    31621 ggcagcgtgt tgatggaaca gcctgtcaaa gccaccaccg ggacgctgta tgacctggcc
    31681 tcaaacacca aaatgtacgc cacgaacttc gccctgcaaa agctgatgag cgaaggcaaa
    31741 ctgcatcctg acgatcggat tgcgaaatat attccggggt ttgccgatag tccaaatgac
    31801 accattaaag gcaaaaacac cctgcggatt tctgacctgc tgcatcacag cggcggtttc
    31861 ccggcagatc cgcaataccc gaataaagcg gtcgcgggcg cgttatattc ccaggataaa
    31921 ggccagacgc tggagatgat caagcgcacg ccgctagagt atcagcccgg cagcaagcat
    31981 atttacagtg atgtcgatta tatgctgctt ggatttatcg tcgagtcggt caccggtcag
    32041 ccgcttgacc gctatgttga agagtcgatt tatcgcccgc tcggcctgac acatacggta
    32101 tttaacccgc tgctgaaagg ctttaaaccg caacaaattg ccgccacgga attgaacggc
    32161 aatacccgcg atggcgtgat ccatttcccg aatatccgca cctccactct ctggggtcag
    32221 gtgcacgatg aaaaagcctt ttattcgatg ggcggcgttt ccgggcacgc aggtttgttt
    32281 tccaataccg gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg gggctatggc
    32341 gatgtgcagc tgttcagtgc ggaaacggtg aagatgttca ccaccagctc taaggaagat
    32401 gccacttttg gcctcggctg gcgcgtgaat ggtaatgcca ccatgacgcc gacgtttggc
    32461 acgctggcaa gcccgcaaac ctacggtcac actggctgga ccggaacggt gaccgttatc
    32521 gatccggtga atcatatggc gattgtgatg ttaagcaaca agccacattc gccggttgcc
    32581 gatccgcaaa agaatcccaa tatgttcgaa agcggtcagc tgccgattgc aacgtatggt
    32641 tgggtagttg atcaggtgta tgcggcgtta aagcaaaagt aataaaaagg ccagtcagta
    32701 atgttactgg ctggtctgag agtgctgaca aacgcaaaac tgcctgatgc gctacgctta
    32761 tcaggcctac gtggagccca caatatattg aattagcacg atttcgtagg ccggataagg
    32821 cgttcacgcc gcatccggcg tgtacaacgc gcacttggtc aacaatctga ggccagtcag
    32881 taatgttact gcctggcctt taatcaatga atcagaaacg cttacagcgc catcaacttg
    32941 tccagcgacg gtgcgaaata atagccgccg gttaccggtt tggtgaaacg caacatcgca
    33001 tcacgcttac catcggtatc gccaaacatg ctcagcagtt gctgctcaat gttatgcaga
    33061 cgcgcgcagt aggcgcagaa gtacagaccg tgagtgccac tggcagtgcc gtacggcagg
    33121 ctctggcgaa caatcttcag ccctttgcca tcttctttca gatcaacgcg ggtgaggtga
    33181 gaggtttccg gacgttcgtc gccgtcgatc tcttcgttgg cctctttggt gcgcccgatc
    33241 atcatctcct gatcgtgaac gctcatccgg ttgagctgat tcaggttgtg ttcccaacgc
    33301 tggacaaaca catagctgcc gcccgcatcc acgccgtctt tgataaccgc cacttcgcga
    33361 cgcgtctctt cacccgccgg gttttccgta ccgtcaacaa agccgctcag gtcacgctct
    33421 tcaacccaac ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc aaaggcttcc
    33481 atcgccgcct gggcgacaga gaagtttacg tcgtgacgca gagaaagaat gtggatcaac
    33541 acatcgaact gggtcgtcgg cgcaaggcct ttaccgtagc ccggaaaatc tttcagctct
    33601 tctgccccaa cgccgccgct cagagcgcgc caggtgttgt taccaaaggc aaccaccgca
    33661 ccaagatgcg cgtccgggaa tttcgcttca aaagttgcca gtttgtcggc aaatgtttta
    33721 ctggccgcac gcagggcgtc aacttcccct ttcacgttgg cttcgatcca aatcgccgcg
    33781 cggcaatgtt ctggcaaaat gccactctga acctgagaca ttgttcctcc tgaaaataat
    33841 aatgccacgc aatccgtggc attgttgagc gctattttac gtgttttttc gcggcggcgg
    33901 tttgttcaga cgcaaattaa cgccgccaga taattttact gactttccag tttttcaggg
    33961 tatcgtccga aggcattaac ccttcaggac cactccattc cccgctgaac tggtagctaa
    34021 tatgttgact gccttcggct ttacattcga cggcacgatt atcatcacca tcagcttttt
    34081 ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg
    34141 tgtcagcagg aatatcgcta tccagcacgt caatgcgact gatcgtgcct tgatcgccgt
    34201 taatcaccat cgccacgttg tcgcccttca tcacttcaaa aaagcgcacc acgttgccgt
    34261 tcgcggtttt cattccgctg cgcaggcgat aatcgccatc aagcgcatcg gcaatggctt
    34321 gttcttgcag tggtgtggac gccgttaatt caccaacgcc ctgctcgctc actttggtgg
    34381 acgacccaaa ccagttccac gggttagcgg cagaccagtt aactgacgac atcgtggaac
    34441 agccggtcag catcaatggc atagcgcata acattaaacg cagcgatttc atgtcacttc
    34501 ctttggttat tcaataacgt tgcttggagt gcaaattcac caaaaagtgc cgttattctt
    34561 ttacttcatt aaaacaagcg cgtaaacgtc gattggtcag cagccagatt aacgccacaa
    34621 tatccgccac taccagcgcc agaccgatac cgctaacgga ttcaccgttc agccacagcc
    34681 acggttgcca gcaaagtaaa accacctgcg ccaacagcaa cagaaaatag agtacacgcc
    34741 aggtgcgagg aaacgtagcc cgccgaccgc tcaacaaaaa cgccagcacc gccggaatgc
    34801 caggaatcag ccccagccag aaattatcgt gatcgggata aaacagattt agcagcgcag
    34861 taccctgctc acgcgacgca ccggcaatga caaacagcac ccaggttcgc gcctgaagca
    34921 atagcacaag ccagaagagc aagggtaaac gcaggcgtcc gtgcgcatca taatggacag
    34981 gatggaactc agtactcttc atcttcaatc aaacgcttac ccagactcag cacgtcggcg
    35041 tgttcatatc ccaggcgttc atacattccg agcaccatgt cgttatcttc cggcacattg
    35101 atctgaattt tcgggcagcc acgagcaatc agctttttct ccagccgatt aagcaacgca
    35161 ttggcaatcc cacgcccacg aaactctgga tgcacgccaa gataataagc agacccgcga
    35221 tgcccgtcat aaccgcccat caccgttccg accacttcac cgtttacctc agcgaccaga
    35281 aacaaactga cgtcatggtt catcttacgc tcgatgtcca tttccggatc gttccacgga
    35341 cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat
    35401 acgcgtatct ccatggtatt cgttaccttt ttgcgggtta aaaggctgat tatggcgtga
    35461 acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa aagtggcata atggggagtt
    35521 gtcaactatt gaaatgaaaa gtaaaacaat tctcaacatc aaaccgtcgt aacggattac
    35581 gcgatacgat ataacatctg gaactttatt attacaactc aggccgtatg agcactttta
    35641 aaccactaaa aacactcact tcgcgccgcc aggtgctgaa agccggtttg gctgccctga
    35701 cgttgtcagg aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc agcaacggac
    35761 acagcaagcc gaaagccaaa aaatctggcg gcaaacgtgt cgttgttctc gatccaggcc
    35821 acggcggaat tgataccgga gcgatcggac gcaacggttc gaaagaaaaa catgtggtgc
    35881 tggcgattgc taaaaacgtc cgttccattt tgcgtaatca tgggattgat gcgcgtttaa
    35941 cgcgttctgg cgatacgttt atcccacttt acgatcgcgt tgaaatcgcc cataaacatg
    36001 gcgcagatct gtttatgtca attcatgccg atggctttac caacccgaaa gctgccggtg
    36061 cttcggtatt tgccctctct aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg
    36121 aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag gatcacctat
    36181 tgcagcaagt gctgtttgat ctggtgcaaa ccgatactat taaaaacagc ctgacgctcg
    36241 gctcgcatat tctgaaaaag attaagccgg tgcataaact gcacagccgc aacaccgaac
    36301 aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt
    36361 ttatcaccaa cccggaagaa gagcggctgt taggcacggc ggcgtttcgt cagaaaatcg
    36421 ccacagcgat tgctgaaggc gtgatcagtt atttccactg gttcgacaac cagaaagcac
    36481 attcgaaaaa gcgataagtt atgaaacccg acgcacacca ggttaaacag tttctgctca
    36541 accttcagga tacgatttgt cagcagctga ccgccgtcga tggcgcagaa tttgtcgaag
    36601 atagttggca gcgcgaagct ggcggcggcg ggcgtagtcg ggtgttgcgt aatggtggtg
    36661 ttttcgaaca ggcaggcgtc aacttttctc acgtccacgg cgaggcgatg ccagcttccg
    36721 ccaccgctca tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttcactgg
    36781 tagtgcatcc gcataacccg tatgttccca ccagccatgc gaatgtgcgt ttttttattg
    36841 ccgaaaaacc gggtgcagag cccgtctggt ggtttggcgg cggcttcgat ttaacccctt
    36901 tctatggttt tgaagaagac gccattcact ggcaccgcac cgcccgtgac ctgtgcctgc
    36961 catttggtga agacgtttat ccccgttaca aaaagtggtg cgacgattac ttctacctca
    37021 aacatcgcaa cgaacagcgc ggtattggcg ggctgttctt tgatgatctg aacacgccag
    37081 atttcgacca ctgttttgcc tttatgcagg cggtaggcaa aggctacacc gacgcttatt
    37141 taccaattgt agagcgacgt aaagcgatgg cctacggcga gcgcgagcgc aattttcagc
    37201 tctaccgtcg cggtcgttat gtcgagttca atctggtctg ggatcgcggc actctgtttg
    37261 gcctgcaaac tggcgggcgc accgagtcga tcctgatgtc aatgccgcca ctggtacgct
    37321 gggagtatga ttatcagcca aaagatggca gcccagaagc ggcgttaagt gagtttatta
    37381 aggtcaggga ttgggtgtaa ctccctcacc cccattcccg catccgctga tgcaacgtca
    37441 acgacggctt ctcggcaaac agctgctggt aatccgtggc aaattgcccc agatgccaga
    37501 atccccactg catggcggcg tcttttaccg ttgtgctttg cgaccacggg cttatcagtt
    37561 cgcggcgtac ggcgttcagg cgaatgcgtt tcagccacgc gtttgggcca atgcctaaaa
    37621 tagcgtgaaa cgcgttttgt agcgtgcggc gactgacatg cagttgatta cacaagtcca
    37681 gcaccgtcac cggctcagac atattttcca gcacatattc acgggcgcgg gaaagtaatc
    37741 tacggtaact ctgatgactg atgctttccg ccgtcaccat tggttgcgcg tcttccagca
    37801 tcgcccccat cgccattagc aaattatccc ccggcacttt tcgcactgca ggctgatgga
    37861 gattttccgg gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc cacagcgcgg
    37921 ctttatgctg ctctttcact tccagcgccg actggctacg cagcatatgt aatacccgat
    37981 ccgggttatg caaaaagtta gcctgccggg tgatgacatc ttcagaaagc accacgccca
    38041 ggatcgtgta atcatccggc gtgctcagtt caaattcagt gccaccaggg cgggtggcga
    38101 tttccgcgct tcccagacat tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa
    38161 tgccaaacca gaacgagttc ggccagacca ggcacgactg acgcagcgcc agaccggtgt
    38221 attcacgaaa aacctgaata tcatcgagta gaatttccgt aaattcacca tgaaacttgc
    38281 ccggatgcag ctgatcgtaa atctgctgcc aggcggtaat cgttaaagca tgttcataga
    38341 catccgttgt ctgtcgttga tgaacattat ccacttcgac cttcggcgtg agcttcaggt
    38401 tttcgggtaa gggttcatga taaagatggt gcaaattggc tgtacgggtc tttttcatga
    38461 tgttaatgcc gggtgttgta ggacacccgg cacctccgac aggttaatgg ggcttgagac
    38521 gataacgact actgcgttta cgtaacgtcc cggcagaaaa gagctgttcg agcgcatttc
    38581 tggctttttc cagcggccag ccaaagtggg cagccacttc tcctgccgtc attccctgac
    38641 gtactgatgt taacagcgcc aacaactcgt cagcagattc cgcgacgata actggcgcgt
    38701 cgggttccct tacgggttgc ttcggctggc ggttaaagcc agtaaccagc cactgggtgt
    38761 catcgtctgg ccgaccgatc tccttgcggc tgatgacacg cccggtacgc atcgcggcag
    38821 cacaacctgc gtccagcgcc gcacgacacg ccgccagatc gccttccacc accagcgtta
    38881 agcgaccagg gtcaagcact tcgtgactga gcagacgaac gttagctgct ttgagcatgg
    38941 catccgctgc atctatcgcg gcgaccatgc cgtccacttc cagcaatccc agtgcattga
    39001 tcatcggcaa cctccgttac gcacgctgga ttggattacg cgcgatttcc agcactgcat
    39061 cggtaaaggc gttacaggct gctttacacg ctgcctggct accggttaaa aatgctgccg
    39121 agtagttggt ttcagacggt ggcgggacat aggtcgccag ctgtacgtcg gcagacttca
    39181 gggctgcatc aatgccatag gtcgcttcca gcggcggtgc caccagatac gccatcggat
    39241 cgccaagcgt gatcccggcg gttgatgaga gataagaacc ggtacgcgaa actacatgtg
    39301 ccaggaatgc cgtatcttgc gcgtcattag cccactggaa agctgcgcca ttttcaatat
    39361 gcgcaatcat cgcatccaga cctgcacgca cttccgccgg gtttggccca ccaagcataa
    39421 tcagcacttc accggcagtc ggtgacgggc cgtgtgccgc tcccgcatac agcgagcgac
    39481 catataccac ttcgaccatc gcttgtttgg tcgcttcgtc agccgcaatg taggtgacgt
    39541 cgtcagaatc ggcagaaatc agcccgaggc tacgaatatg cggcggcaat tttagttcac
    39601 gcgcaaattc agcgttaacg gaggcaatca cccgcatggc ggttaccgac ggtcgaatca
    39661 aatctaaagc tggcatgatg cctccttatc gggtcatgtt gatgccggat gctttctgct
    39721 ccagcatacg tttcgccaaa tccacaatga cggctgcggc ttcaaccggc ggcgtgccgc
    39781 cctggtgaat gttagagata caggtgcgat cggcctcaac ggtggtcgcc atacgcggcg
    39841 agtaaacggc gtagcaggag aggctttctg actgccctaa ccccggacgt tcccccacca
    39901 gcaggatcac cactttcgcg ccgaggatct caccgatctg atcttcaatc ttcacgcgac
    39961 cataacgaac aaagaatggc gtgccgactt tcagcccggc ctgtttcagg cccgccatca
    40021 gcggcggcag gatctcttcg tagttgacgg tgatggcatc agttgacagg ccatcagaaa
    40081 taacgacctg tacgtccgga ttagcaacac actgtgcttt cagcgcttca acagcttctg
    40141 cacacaggcg gcggcccata tccgggcgag tcaggtagag atttttgtcg ctaatttcag
    40201 agcgtacttc cagcaagccc tgcgctttca cccactcttc cggcacttct ttcagtacgg
    40261 tatctttcga acgagagtga tcggccagga aacgcagcag cgcctgggta cgtggacgcg
    40321 gaccggcacg accggtacaa acgcgggcca cggtgctgcg gcgcagttct gttaatacgt
    40381 ctgcgcgatg cgggttttca acaccaatcc acgcttttgc ttcagcggaa cctaaatcca
    40441 gcgcacagct ttccgaggtc accggtgccg cacagttggt ggtggcgcac tttgcttctg
    40501 acggcgcggg ggccgtttgt cccattgacg ccatcacgct gcgtacaatt tcttcaatct
    40561 gtttttgatc catgatgtgt tatctccgcg tcatcagaag aacagtgacg gatcgcccgc
    40621 ccgtttggtc aggcgaccgt ttgccataat gcccatgctt tccagccagc gttcaaactc
    40681 cggtgacgga cgcaggttga gtaactgacg cacagtggcg gtatcgtgga atgcggtggt
    40741 ctggtagttg agcatgatgt catcacccag cggcatcccc atgatgtagt tgcagcctgc
    40801 ggtggcgagc aggatcatca ggttttcgtt gaggttctgg tcagcgtcag cgtggttggt
    40861 gtagcagcag tcacagccca tagagatgcc gctcagcttg cccataaagt gatcttctaa
    40921 gccagcacgg ataatctggc ggtcgttgta gagatactcc ggcccaataa agccgaccac
    40981 ggtgttgacg ataaacggat cgtaatgacg cgccagccca tagttacgtg cttccatcgt
    41041 cacctggtca gcgccgaagt tagcgccagc ggatagcgca gagccttgtc cggtttcgaa
    41101 gtagaggcag ttttccccgg cgatacgatt gaactctgcg cccactgcgc gcgcttcgtc
    41161 gagcatcgcc agttccacgc caaactcttt cagccctttt tcgctgccac agatactctg
    41221 gaaaatcagt ccgccaggcg cgccgcgacg aatcgcttcg atctgggtgg tgacgtgcgc
    41281 cagtacgcag ccctgagttg ggatgttgaa tttgtcgatc acgccataaa tggtatccag
    41341 cacgcggctt aagttttcca cgtcgtcagt taccgggtta acgccgatca ccgcatcgcc
    41401 caccccaaag gaaagccctt catagatttg cgcagcgata ctttgcacgt cgtcacgggt
    41461 atcgttcggc tgcaaacggg cgctaaaggt gcccggaata ccgatggtgg tattggcctt
    41521 tttgattacc ggcattttct tcgcgccgta gatcaggtcc gcgttggagc aaatcttcgc
    41581 taccgccgcg accacttccg aggtcagccc tttgcgggta aaggcaatgt cgtccacgct
    41641 ggtttcatcg ctcagcacat actcacgcag ttcgctgatg ctccagtttt taatctggtt
    41701 gtaggccgtt tcgttaacgt cgtcctgaat cagccgcgtc acgcagtcat cttcataggc
    41761 aatcaccgga ttattgcgga tgtccgctac ggtcatttcc gacaacacct gctttgccgc
    41821 cacgcgctcc tgtgaacttg ccgctgcaac gcctgccagc acatcccccg aacgcagttc
    41881 gttggcttta gccagcacct cttttacatc cttaaactga tatacattgc cgaacaatgt
    41941 ggtctttagt ttcataagtc gttccctcag gaaggaaatg cgagtgattt caccgtcacc
    42001 ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca
    42061 atcacttcgt caatgactgc caacgggagt tgttgtagct gtgggcgcaa caacatgccc
    42121 agagctttac caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc
    42181 gcgacgaaat cgaccagcgc gttgatgacc gtcagtgccg cggcgtaacg cacaggcagc
    42241 gaggcgggaa gcgccagcac gtacgcgtca gttttgggat caagatccag ctgaagcagc
    42301 gcctgttgcc aggcactcac cagatccgtt tcatcaatcg ggatcgccac cggcaaattg
    42361 cgcagcggca gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt
    42421 gcacccgcgc caatcaccgt ggcgcgtacg gtttgcgccg gaaactgcac attcatctca
    42481 cgcaggcgcg gatggtcatg cagcgccgtt gccagaagtg ggccaatatc ggcaaaacag
    42541 aacgggtcgg cgggctggtg gcgataacat tcgcccacgc ctccagaaag cgtaatgatt
    42601 tcgggcgtaa cacctgcggg cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa
    42661 agcgttccgt caatcacttc gacaatcagc gccgccatcc gccgggtaac ctgcaccagc
    42721 tgcgcgccgg tcagcgaacg ggcgtcagtg cctgcaccga agcactcatc cacaatcatc
    42781 tgccccggtt tatgagcgta aaccacgcgc ccctggctgt cggtttccag caggcgacca
    42841 ccgacgttga gacaggcagt gccgctgatt tttccggcat cgaacagggc gtagttcgcg
    42901 gtgccaccgc cgatgtcgat attcagtacc cgacacagcc gttgttcaga aagggtttgc
    42961 gccccggctc cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct ggcgacgaca
    43021 aaatcgccca gcgactgaga gagcgccatc accgccgggc gagcattgcg ggttttcgcg
    43081 ctttcaccgg tgatgatgat ggcaccagaa tcaacgcttt ccggcgcaat gcccgcagtc
    43141 tgatattgct ctaaaatcag cgatttcagt tccgcttctt ttaaaccacc ctgtttatcg
    43201 acaggggtaa agaacaccgg gctttgccag ctaatttcgc gtttgatgaa ttcgtagcgc
    43261 ggcacctgcg acaccgccgc acggttaacc agctccaggt gggagaaaat cacctgggtg
    43321 gtggtggtgc cgatatcgat accgacgctc aatagctggc gagtgttcac gattgtgcct
    43381 ccgcttcggt tttagtcgcg gtcgcgtctt cttttggcac cagcatcatc gccacgccaa
    43441 tcgccgttac gccgccgatc aacttgccga caatcatcgg gaagatcatg gcgttcatgt
    43501 tggcagcggc gaagcctaag tggtcgccca gggcgaaagc agcggaaacg gcgaaggcgc
    43561 agttgatgac tttgccgcgg gtatccatct gcttcatcat gccgaacatc gggatgttgt
    43621 tggcaagcgt tgccaccatg ccggctgccg cgatgttgtt catattcagt actttaccaa
    43681 cgctcatcag cggtttttca aaccagcgag tcagcagcag caccatcgga tacgccccta
    43741 acagaacgca ggagatagaa ccgataactt caatggcgcg catcacctca ccgggtttat
    43801 cgccaggggc cataaagata ggatccagac cggggatcag ttcccagcca agcaggaatt
    43861 tcactaccgc agcggcaaga ccgagggtga tcaatgcaac gaggaatttg gcgaagatct
    43921 ggaagccgtt gatcattttt tccgggatga atttcagccc cagcgccacc agaatcgcaa
    43981 caatgatcac cgggatcatg ttcatcagga tcagggcgaa agtgaattcc accggctggc
    44041 cgttgatctg cacaccggag tacatagcaa ccagaccacc agcgatacaa ccaatcggaa
    44101 tggtcacaat gcccgccagc acgccgagcg ccagataacg acggtcagaa ggttcgataa
    44161 tgccgagcgc caccggaatg gaaaacacaa tcgttggccc catcatcgac ccgagaatta
    44221 acccagagta tagccacgcg gctacgtcgc cgcccgccag ctctttggcg aggaagaagc
    44281 cgcccatatc gcacgccagc agtgttccgg cgaacatcga tgggttagcg ccgagcattt
    44341 cgtaaaccgg aataattacc ggcccgagaa cgtgagccag taccggtgcc agcgcggtca
    44401 taccgaccat cgccaggccc agtgcgccca ttgccataaa gccttcttcg aactgaccgc
    44461 ctgatccttc gatactttta ccgaacttac cgaggaaacg agcagaaccg ccgaactgcg
    44521 acaggatcct gtctacggca gctatcagca taaagaacat catgatgtac atgatgattt
    44581 cgttaattcc catcgccttt actccctgtt agttgttatt tattggcgga tgcggcgtaa
    44641 acgccttatc cgccctacat gtgcaatccc gtaggctgga taagacgcgg caagcgtcgc
    44701 atccggcaat tgcaccgcgc cactggcgga tgcggcgtga acgccttatc cgccctacat
    44761 gtgcaatccc gtaggctgga taagatgcgg caagcgtcgc atccggcatt tattgcgccg
    44821 ctgcgtacag gccgacaatc tgctccaggc tggcggtacg cgggttactg cgcagacaaa
    44881 tatcttccag cgcggcctgc gcccatgcgc cgtaatgcgc agatgtcgca ccaacatcgc
    44941 ccagtcgttt accaatccca acttccgcaa tcagctcact taccgcgtta atagcgtcac
    45001 gatcgtcgga ttttttagta cgcagtgccc gaccaatctg actaaagcgt tcgcgacaaa
    45061 ccatccggtt aaattccatc accgttggca gcaacatggc gttcgccaga ccgtgtggaa
    45121 tatgcagcgc cgcgcccggc tgatgcgcca tcgcgtggca caacccaaga cccgcactgg
    45181 aaaacgccat tcccgccata catgaagcca gcaacatgct ctcgcgcgcg gcaaggtcgt
    45241 gaccgtagcc caccgctttc ggcagcgatt tgccaatcat cgcaatggca ccaatcgcca
    45301 gactgtcggt aaacggtgta gcgttcaggg cgctgtatgc ttcaatggca tgggttaacg
    45361 catcaatgcc ggtcatcgcc gtgacatgcg acggcacacc ttcggtcaat gcggcgtcga
    45421 ggatcgccac atccggcatc agcgaggcat gggctaacac ctgcttgcgc ccgctcaccg
    45481 cgtcgataat caccgttaca ttggtggttt cagagccggt tccggcggta gttggaatgg
    45541 caatcagcgg caagcgcggt tgcagaacgc tggtttctga catctctgcc agcgtgctat
    45601 ccgggttcgt caccagcaac gtcacggctt tcgccgcatc cagcaccgag ccgccgccaa
    45661 acgcgatcac cccatcacag cctgactcac gcaactgcgc cacggctgca cacacgtcgg
    45721 taatgcacgg ttcgcccacc ggacatggcc agagcgtcat ggcgatacct ttaacggtca
    45781 ggctacgcgt cagcccggcg gtcatccctg cctgatgcaa aaagctgtct gccatcacga
    45841 acagatgttt cagcccacgc gtttgcgctt gctgtccgca actgctcacc gagcccggac
    45901 cgcaaagcgt caccggtgga acgctaaatg tttttacccg ttgcagattc agggtatcga
    45961 acgcctgaaa gagcgcggtc tgcaattcat tttgcatata gcccctccgc tttctctcgc
    46021 ccactactgg cgatcgccag cggggtcata aacaaactgt gctgcggtaa atgcacctgt
    46081 aacgccggga attgtttgcg aaacagttcc gccacgcccg gttgcataca agagccgccc
    46141 gccagccata aatcagtaat gccctgccct tcaatatgcc gggcgacaat gtccgccatt
    46201 ttttcgtaca ccggtttcac cgcaggccaa atctcttcac cgtgaccgcg cttgtactgc
    46261 tccgcctctt ccagcgaaat acggcgattt ccggcgagag tcagagagat gtgatgtccg
    46321 ccggtggctt catccgccga gtacgtcacc ttgccctttt tcacgatggc aatgccggta
    46381 gtgccgccgc cgatatccac tacaccggcg ttgtccagtt gcagcagatc cgcaactgcg
    46441 gttggctcat ccagcacatg gctcacttcc agtccggcag attccagcac gttgatggaa
    46501 atacgcgggt ccgtacctgg aggaaatgaa gtcgccgcat ggctaaaacg acgaccgaat
    46561 tgctgctcga gcgtgtcgag atggcgacga acaatggtga cagcgccgaa gaaatcccag
    46621 acgatgccgt cgcgaaccac atcggcccag tcgaggcata ccgccactgg ctgaccgtcg
    46681 cggtcgacaa ccatcgacac cacatcgcag gtgcccagat ccacccccag ccacagcgga
    46741 gattccgtcg cggcgggcgt ctggttacac agcgttgccg ccgtttgcag acgtggggtg
    46801 agccattgtt cgtcgtgcgc catctgttac tccttaaaca atgcgaaacg catcgactaa
    46861 tacacagcga cgcagacgga caaacgtacg cgcgctggtt accccttcac cggttggcgt
    46921 ggtgatggtc atggtggtcc agccttcccc gcccagcccc agcccggcaa tgcacggtcc
    46981 gttcttaacg aaaatgctgg tatcaatagc attcgccatc tggttcatgt tttcgatgtt
    47041 gcgcgagtgc attgccgccg tgtggtggca accgccttcc agtttcaccg ctagcgcaat
    47101 ggcatccgcc acgttggcga cgcgcacgac gggcaacacc ggcatcatca gttcagtcac
    47161 ggcaaacgga tgttctgcgg tggtttccac aaacagcagg cgcgtttctt gcggaacttt
    47221 aaggccgatt gccgccgcga ttttgcctgc gtcgcgacca acccagtcac ggctgacggt
    47281 gccttttccg cgctcgtcga tatttttcag caacaccggt tgcagctgct gcgcctgttc
    47341 tgcggtcagt ttcaccgcgt gctggccttc catcagacgc atcagttcat cggctacgct
    47401 atcaacaaca atcagtacct tttcgtcggc acaaatgatg ttgttatcga aagaagcgcc
    47461 tttgacgatg gactgagcgg cacgggcgag gtcggcggtt tcatccacca ctaccggcgg
    47521 gttgccagcg cctgcggcaa tcagacgttt attggtgtgt ttacgcgccg cttctactac
    47581 cgcttcgccg ccggttacca ccagcaggcc gatacccgga aacttgaaca agcgttgcgc
    47641 ggtttcgata tccggatttg ccacagtaac cagtaagttt tccggcccac ctgcggcaac
    47701 aatcgcctgg ttgagcagcg taatcgcccg ctgggagact tttttcgccg ccggatgcgg
    47761 ggcaaaaatg acgctgttgc ccgcggcaat caggctgatg gcgttgttaa ttacggttgc
    47821 cgccgggtta gtggaaggcg tcaccgaagc caccacgccc cagggtgcgt tttcaattag
    47881 ggtcaggccg ttgtcgccag tcagcacttg cggagagagg cactcaacgc ctggtgtgcc
    47941 gcgcgcctga gcgacgtttt ttgcaaattt atcttcaacg cgccccatgc cggtttcact
    48001 gacggcaagt tccgctaaat ctctggcgtg tttttcgcct gcttcacgaa tggcagcaat
    48061 ggctaactgg cgcattgcca cgctttttaa cccttgctgg gcgactttgg ctgccgcaac
    48121 ggcgtcatcc agggacgcga aaacgcccat ctcatgaacg gcggcggacg gcgtgtcact
    48181 gctttgcatt ttcagcagta ccgctttcac cacctgttca atatcctgtt gattcatgat
    48241 gttctgcctt atttgtggaa aattacctga ccgccagaca ccacctcatc gacaatgcca
    48301 atcacgcaca gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc ggaactgcca
    48361 ctcaccagta acacccactc cccggttccc gcgccaatat tgtcgatggc gacggcgcat
    48421 tgcccgtcag gattaccttg tggatcaatc atttccacca tcagcaattt gtcatgcgcc
    48481 agtccgtgat ggcgtacggt acaaacaatt tgtccagtga cgactgccag tttcataccc
    48541 gcctccgtgg cgtatttcag gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa
    48601 aaggagagag cgtgacgccc gaatcgacgt cacacagggt gattacaggt tgctgctatc
    48661 gcctttcagg ccgatcggga agacttcttc caggtcgccg tgcggacgcg ggataacgtg
    48721 tacagatacc agctcgccga tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt
    48781 acaggctgca acatcaccac gaaccatggc agtacacagg ccgccgccaa tctgcttaac
    48841 accaaccagc ttgacgcgcg cagctttcac catcgcgtca gaagcctcaa tcagtgcaac
    48901 caggccccgg gtttcgatca ttcctaatgc ttccattgtg ctttcctctt tatcagggtc
    48961 cagaacggga ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc
    49021 tgacgcggca cagctgccac cagcgccagc tcgataattt cctgcacgct acaaccacga
    49081 gagagatcgt gcatcggcgc ggcaagtcct tgtatcagtg gcccgacggc acgatatccg
    49141 ccgagtcgtt gtgcgatttt gtaaccaata tttccggctt ccagcgacgg aaaaaccatc
    49201 acattggcct tgccctgtag cgggctggca ggcgcttttt gcgccgccac ttccggcacg
    49261 aaggcggcgt caaactgtaa ctcgccatcc accaccagct ttggtgcgcg ctcacggacg
    49321 atttctgtcg cctgctggac gttagcaaca caggggtgac gggcgctacc attgctggaa
    49381 aacgacagca tcgccacgcg cggctcttct ccggtgatgg cgcgccaggt ttcggcactg
    49441 gcaagcgcga tatccgccag ctgcgccgcc gtcggctgtg gcaccacgct gcaatcggca
    49501 aagcccaacg ccgggccgct gtactgtggc aacatcagga aaatggaaga gagcgtttta
    49561 cagcctggct gcaagccgat aatgcgtaat ccggcacgca gcacatttgc cgtggaagag
    49621 aggttgcccg cgatacagac atccgcttta ccggcgctga ccattgctgc ggcgaacatc
    49681 agagggtcgg taagtttttc cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc
    49741 cagcgatgag caaattcttc ccgcattgcg aggttgccat gcggatcaat cacctgtagc
    49801 ccgtccatcg ccacgccgtg actgagcgca aactgacgaa gttcaaacgg attggcgacc
    49861 agaatgggcg ttgccagacc ttgttgatgt aaatattgcg cggctttcag cacacgttgg
    49921 tctaacgcat ccggaaaaac cactctggcg ggcgctcgca acgccagttc acgacaacgt
    49981 tcaataatca ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt agcggctgct
    50041 tcgttacgct caggatcatc atcacgtaga ccgtgctcga aaggcggttc agcgcctgca
    50101 aaatgtccgg gcgtaatact tcaaagctgc gggtgataaa gacctgcgcc gctacggttt
    50161 cggtttcccg cactttggtg cgtagcagat tgagcaacgc agcatcgcgc ccgtggctgg
    50221 cttccggcac cagatgatcg tggtcgagat agcgcagcgg ctgatgagaa agccgatgca
    50281 gatcttcgtc agaaagcccg acaatcgcct ggcagcccag cggttccccc agcgcatcgg
    50341 cgcgcataat gttgccaaga cgcgaacgga tatccgccag ccacggctgc cacggttccg
    50401 ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgagaacc gcgcgaaaac
    50461 ccagacgcgg atcgcttttg gcgaccattt tttccgccga caggtgagtc agcgtatccg
    50521 gcttttttgc caccggctgg cgacacagtt cgcagcacgc ctgcggatgt tcatcgctac
    50581 tggtcagccc atgaacgggc tgcggctgct gctgttcatc gtcaacaaac aggcggccct
    50641 gctcgtcaat aaacttgatg cgcagatggc ggctttccag caactcccgg gcagagggcg
    50701 tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta tggttcgctc
    50761 ttagccatgc ttcggtgatg aaatctttca tagggattgc cagttagccg gccaggcgac
    50821 atacaggaat ttcacgctgg atgtcgtacc aaattcgatg ctggagcctt tcgggataaa
    50881 catcacgtcg cctgctttgg cgatcatggt ttggccttcg tggcgaacat gcagctcccc
    50941 ttccagcacc atgtcgattt cgtcgtagtt cagggtccac gggaagaatg cgttctccca
    51001 ctgcataaaa ccggcggcca tgctgctgcc gtcgtctccg gtcacaagat cagtcaagcc
    51061 cacgcaatgc ggctctgcgc cgtcgaaacg accaaacttg acgctactgc catcaatcac
    51121 tttgatgccg cctttgccgg tcaccgattt gaagctcggc tgcattgcgc cctgctccag
    51181 cgactgtttt tccttcatca ctttttccat cagctgtgcg accaggcttt cggtaaactg
    51241 gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg ttttgtcggc
    51301 tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt cggtgatggt
    51361 aaaacccagt agatccgcca cttcacgggc ttccggggta ataatgctgg cgcgcagaac
    51421 cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag
    51481 ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc agttgctgca
    51541 cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaaaccc gtctcaagca
    51601 ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg atgacggcga
    51661 tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt tccgggtcat
    51721 ttgcgccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt aaggcgtgat
    51781 accagcgggg atggttaaaa tattcacccg gcgtatcaat atcgccctta tcattaaatt
    51841 ccacggcctg tgtttttctg gcgagggtat aattcccctg caacgcatta aataacgttg
    51901 tttttcctgc cccgacgcta cccacaaacg caattcgttt catgaatgcc tcgtattaac
    51961 ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg acggtttgtg
    52021 ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg ctgaatcggt
    52081 cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccggcg atcatcgccg
    52141 tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg ccgattttct
    52201 tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt ttgccgggca
    52261 caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt
    52321 atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt taaaaggtga
    52381 ggtagatcac taagaatgga gagatattcg ctgtggtgca taaactttca tatgcaacgc
    52441 atgaatataa ataaaataat atcgagatat tatatagact gctttttttt attattcgca
    52501 aactcagtaa taaactttac taatatattg cagctaaact gcttaccctg aatattcagg
    52561 gtaagcgtga gagttaaaaa aaattacagc ggttgggttt gcgcttctac cacggccagc
    52621 gccaccatgt tgacgatacg acgcaccgat gcgatcggcg ttaacacgtg aaccggtttc
    52681 gccacaccca tcagcaccgg gccgacagtc acaccttccg agctggaaac acgcagtaag
    52741 ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca gaatattggc ggaacctttc
    52801 aaagagctgt ccggcatacg gtcgttgcga atcgcttcca ccagcgctgc atcgccgtgc
    52861 atttcaccat caatcatcag ttctggtgca cgttccctga ccagttccag cgcctgacgc
    52921 attttgctcg acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa caaagcaacg
    52981 cgcggctcaa taccaaaacg acggacagtt tctgccgcca tcaaggtgat ctccgccagc
    53041 tcttctgcat ccggttcatc attaacatat gtatcggcaa taaaggtgtt accactcggc
    53101 agcagcagcg cgttcatggc acctgcggtg tgaacgccat cgcgataacc aaagacattt
    53161 ttcaccacgc taaaatgttc atgataatca cccaccgtac cgcaaatcat tgcatcggct
    53221 tccccacgct gaaccatgat cgcgccgatc actgtcgggt tactgatcag cgcccgctgc
    53281 gcctgttcct gagtgacgcc gcgacgcttc atgatctgga agtattcggt ccagtactct
    53341 ttaaagcgcg gatcggattc gttattgacg atctcaaaat caacgcccgc tttgatctgc
    53401 aagcccagtt tctgaatgcg catttcgatc acgttcggac gaccgataag gatcggtttc
    53461 gccagtccca gcgttaccag ttcctgagtg gcatgcagaa cgcgcgcctc ttccccttcc
    53521 ggcagaacaa cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt cataaacagg
    53581 ttggttttgt aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc agcaatcgga
    53641 cgagtcgcca cgcccgactc catcgcggct ttagcgaccg caggagcgat cttaacgatc
    53701 aagcgcggat caaacggttt tggaatgatg tattccggac caaagctcag atcctgatcg
    53761 ccatacgctg aagccaccac ttcgctctgt tccgcatggg cgagttctgc aatcgcacgt
    53821 accgccgcca gtttcatctc ttcgttgatg gcggttgcgc caacgtccag cgcgccacgg
    53881 aagatgaacg ggaagcacag gacgttgttc acctggttcg gatagtcaga acgaccggtg
    53941 caaatgatgg catccggacg cacttctttc gccagcggcg gcagaatttc cggttccggg
    54001 ttcgccagcg ccaggatcat tggcgcacga gccattttct tcaccatttc ctgggtcagc
    54061 actttcgggc cggaacagcc caggaaaata tccgcgcctt caatcacatc atcgagggta
    54121 cgtttgccgt catccaccac cgcatatgcg gctttggttt ccgccatgtt tggctcacgg
    54181 ccctgataga taacgccttt tgaatcgcaa accacgatgt tatgtttttg cagacccagc
    54241 gctaccagca ggttcataca ggcgattgct gcggcacccg cgccggaaac caccatccgc
    54301 acgtcggaga tgtttttctc caccacgcgc aagccgttga ggatggcggc agtgctgata
    54361 attgccgtgc cgtgctgatc gtcgtggaat accggaatat tcatccgctc gcgcagtttc
    54421 tgttcaatat agaaacattc tggcgcttta atgtcttcga ggttgatgcc gccgaaggtt
    54481 ggtgcgagcg cggcgacaac ttcaataaat ttgtccgggt cgagttcgtc aacttcaatg
    54541 tcaaatacat caatcccggc gaatttctta aacagaacgc ccttgccttc catcaccggt
    54601 ttgcctgcca gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt agagatcacc
    54661 gccaccaggt taccgcgggc ggtatatttg taggctttta acgggtcttt ttcgatttca
    54721 agacaaggtg cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg tgttgccaga
    54781 ggcttggttg gagaaacctg gattttccct ggaactggaa attcatggaa atcaagtgca
    54841 ctttgtttta actggtcatc catttggttg ttcctttcac gtaacgttca caaataaagt
    54901 gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta ttaccctgcc ggagtctaca
    54961 gactttgagc aagtccaaac tctcaccatt aatataatgt tttggtaata atcctataac
    55021 actgatgtta cctgcttaat ccagcaatac catgcctgtc tgctatgctt ttttgatgcg
    55081 tttagcgaaa tttctcagaa gtgtgaatta acgcactcat ctaacacttt acttttcaag
    55141 gagtatttcc tatgaacgag ttagacggca tcaaacagtt caccactgtc gtggcagaca
    55201 gcggcgatat tgagtccatt cgccattatc atccccagga tgccaccacc aatccttcgc
    55261 tgttactcaa agctgccgga ttatcacaat atgagcattt aatagacgat gctatcgcct
    55321 ggggtaaaaa aaatggcaag acccaggaac aacaggtggt cgcagcgtgt gacaaactgg
    55381 cggtcaattt cggtgctgaa atcctcaaaa tcgtacccgg tcgcgtgtca acagaagttg
    55441 atgcacgcct ctcttttgat aaagaaaaga gtattgagaa ggcgcgccat ctggtggact
    55501 tgtatcagca acaaggcgtt gagaaatcac gcattctgat caagctggct tcgacctggg
    55561 aaggaattcg cgcggcagaa gagctggaaa aagaaggtat taactgcaac ctgacgctgc
    55621 tgttttcttt tgcacaggca cgggcctgtg cggaagcagg cgtttttctg atttcgccgt
    55681 ttgtcgggcg tatttatgac tggtatcagg cacgcaagcc gatggacccg tatgtggtgg
    55741 aagaagatcc gggcgttaaa tcggtgcgca atatctacga ctactataag caacaccact
    55801 atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga acaaatcctc gccttaaccg
    55861 gctgcgatcg actgactatc gcaccgaatt tactgaagga gctgcaggaa aaagtttcgc
    55921 cagtggtacg taaattaatc ccaccttctc agacgttccc acgcccagct cccatgagcg
    55981 aagcggagtt ccgttgggag cacaatcagg atgcgatggc ggtagaaaaa ctgtctgaag
    56041 gcattcgtct gttcgccgtt gatcaacgca aactggaaga tcttcttgcc gccaaactat
    56101 aaaccagcca cggagtgtta tatgtcccga aaagaccttg ccaatgcgat tcgcgcactc
    56161 agtatggatg cggtacaaaa agccaactct ggtcatcccg gcgcgccgat gggcatggct
    56221 gatattgccg aagtgctgtg gaacgatttt cttaaacata accctaccga cccaacctgg
    56281 tatgatcgcg accgttttat tctttccaac ggtcacgcgt cgatgctgct ctacagtttg
    56341 ctacatctga ccggttacga cctgccgctg gaagaactga agaacttccg tcagttgcat
    56401 tcgaaaaccc caggccaccc ggagattggc tatacgccag gcgttgaaac caccaccggc
    56461 ccgcttggac aaggtttggc gaacgccgtc gggctggcga tagcagagcg tacactggcg
    56521 gcgcagttta accagccaga ccatgagatc gtcgatcact tcacctatgt gtttatgggc
    56581 gacggctgcc tgatggaagg tatttcccac gaagtctgtt cgctggcagg cacgctggga
    56641 ctgggcaagc tgattggttt ttacgatcac aacggtattt ccatcgacgg tgaaacagaa
    56701 ggctggttta ccgacgatac ggcaaaacgt tttgaagcct atcactggca tgtgatccat
    56761 gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc gcaaagcgtg
    56821 aaagataagc cgtcgctgat tatctgccgt acggtgattg gctttggttc gccgaataaa
    56881 gcaggtaagg aagaggcgca cggcgcacca ctgggggaag aagaagtggc gctggcacgg
    56941 caaaaactgg gctggcacca tccgccattt gagatcccta aagagattta tcacgcctgg
    57001 gatgcccgtg aaaaaggcga aaaagcgcag cagagctgga atgagaagtt tgccgcctat
    57061 aaaaaggctc atccgcaact ggcagaagag tttacccgac ggatgagcgg tggtttaccg
    57121 aaggactggg agaaaacgac tcagaaatat atcaatgagt tacaggcaaa tccggcgaaa
    57181 atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt acgggccgat gctgcctgag
    57241 ttgctcggcg gttcggcgga tctggctccc agcaacctga ccatctggaa aggttctgtt
    57301 tcgctgaagg aagatccagc gggcaactac attcactacg gggtgcgtga atttggcatg
    57361 accgctatcg ccaacggcat cgcgcaccac ggcggctttg tgccgtatac cgcgacgttc
    57421 ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg cggcactgat gaaagcgcgg
    57481 cagattatgg tttataccca cgactcaatt ggcctgggcg aagatggtcc gacgcaccag
    57541 gctgttgagc aactggccag cctgcgctta acgccaaatt tcagcacctg gcgaccgtgc
    57601 gatcaggtgg aagcggcggt gggctggaag ctggcggttg agcgccacaa cggaccgacg
    57661 gcactgatcc tctcaaggca gaatctggcc caggtggaac gtacgccgga tcaggttaaa
    57721 gagattgctc gtggcggtta tgtgctgaaa gacagcggcg gtaagccaga tattattctg
    57781 attgccaccg gttcagagat ggaaattacc ctgcaagcgg cagagaaatt agcaggagaa
    57841 ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata ttttcgacgc ccaggatgag
    57901 gaatatcggg agtcggtgtt gccttctaac gttgcggctc gcgtggcggt ggaagcaggt
    57961 attgccgatt actggtacaa gtatgttggt ctgaaagggg caattgtcgg gatgacgggt
    58021 tacggggaat ctgctccggc ggataagctg ttcccgttct ttggctttac cgccgagaat
    58081 attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg cctgatggtg attgccggat
    58141 gctgattgcc ggatgcgacg ctgacgcgtc ttatccggcc tacagcgtct tatccggcct
    58201 acatgtcccg ccattttgtt taacgggtga tccacaacgt gggccaggcg tctggcccat
    58261 gccagttatc gcaggtgggt tctgcggcgt aacgcaccag gcgaaaacgc tgaccgtcaa
    58321 agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct aaggtcacca
    58381 gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc gtctcctgac
    58441 cgttgttgaa cggcaaacgc aaccgaaccg ggcgcgaagc tagtggcttt ttacgcgaca
    58501 caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagcg
    58561 ctttatcatc agtcagcgca gtgacattca cctcacgacg caatggatca agcgagcagc
    58621 gcagaccatt catccgccag tttccataat ccagcaaatc gttgcgttct tcgagtgaga
    58681 gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgcg ggcggtacgc
    58741 tgagcggcgg ttcgtcccct ttcttgatcc acgcggtttc actgccaacg cgcttttgct
    58801 gagcatcaat aaacaacaac gctgctttta agccactcag agaaatggtc tgatcgccat
    58861 cgcgtaaggt gattgccttc ccttcctgaa tcatctgcaa aaacgcggtg atggttgccg
    58921 tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt aacgccaacg
    58981 gctcgccatc taacagcagc cgtggcgcta tctccccttc tgacgcctcc ggcgacttca
    59041 atccgccgcg ctcaatacgt aaaacggcat cggtatgcgc cccggcgctg cggctcaggg
    59101 tcatcaccag tccattatga tcgcccgtat tacgcgccac gcagaaattt tggttattac
    59161 aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc aaagacgtcg
    59221 gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag
    59281 aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcgga
    59341 tttgtcggat tgagccaata ataaacaacg aaatattatt gttatttttc agtccattaa
    59401 atgtgacgtt tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt
    59461 tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc
    59521 gacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa aaaagtggat
    59581 tagctcagtc acaccgcctg gcgacatata cagttcaaat aatttgcgca cttcacccac
    59641 ttcatagccc gtctcttcaa tcgcttcttt gcgaatacac acttccggtt cgtcgttatc
    59701 cagcagcccg gcgcagcttt caatcagctg cccgctttca ttgccattaa cccaggtagc
    59761 gacacggaac tgacgaatca gaaccacggt ctttttcttc gtgttgtaca ggaggatcgt
    59821 cgcaccattg ccgcgatcgt acacttcacg tttatggcgg ataacttcgc cgtctttgcg
    59881 cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt
    59941 aatgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatcttac
    60001 gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttaccccca
    60061 gccagtcaat aatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat
    60121 ccgcaccacg cacggcgtca ccaccagcga agattttcgg attggtggtc tggtaacggt
    60181 actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact
    60241 ccagccacgg catcccgtgc ggattgaagc caaacgccat aatcaccgcg tcggctggca
    60301 tgacaaattc actgccttcc accggcactg gacgccgacg cccctgggca tccggctcgc
    60361 caagacgcgt gcgcaggaaa cgaatcccgc agacgtgacc ttgttcattc agctcaagcg
    60421 ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc ttcacttctt
    60481 tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc
    60541 cgtggcgcag tgcggtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga
    60601 cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc cccatcacct
    60661 gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg
    60721 gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt
    60781 ccaaaagcga atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc
    60841 ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag
    60901 gaatgccgaa agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcaccccca
    60961 cgccattgcg ggtcagaaca tccgcacagg ccagccctgc cggacctgca ccgataatcg
    61021 ccacccgctt gtccactttg gtgacatggc ttaagtcagg acgccaacct ttcgccaacg
    61081 cctgatctga aatgtagcgt tcaatgttgc cgatagttac cgcgccgtgc tcatcgcgaa
    61141 tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta atttccggca
    61201 gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt
    61261 caatccactg cggtatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc
    61321 cgcacttaag gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat ggcagataaa
    61381 tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atccggctcg ccacgcgcgg
    61441 gcgttgcctg catttgttcg actttactca ttaccggcat ttcttgcgcc gcggtactgg
    61501 catgccacgg ttgatgttcc tgacgcgcgg tgcgcaagcg gcgggatttc gccatgccgg
    61561 agagtgcgac gtcagtgacc agttgcagcg cgtccgccgg gcaattctca acacaggcag
    61621 gaccgttttc gcgccccgca caaaggtcgc atttatgcgc cgtggctttt acttttcctg
    61681 ccgcgacggg tgtcaggacg atttgcatcg taccaaaagg acaggccacc acgcaggatt
    61741 tacagccaat acacttttgc tgattgacct gaatgctgtc atcaacgtgg ctgattgcgc
    61801 cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc actgcactac
    61861 gttgctgttg atgtttgata accgtaattc ggggatgaaa atggtgttgg ctcaggacat
    61921 gttgctcatc attgtgagcc atgacacagg cgatttcaca agcatgacaa cccagacatt
    61981 gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc
    62041 ttattcttta tatgagtgtt gttattaccc gacttacagg ggaatcggca atgttcatgt
    62101 gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga
    62161 ctaaattgcg tttcagcgaa ctggaacatt aatgattttt tgtggagaag acgcgtgtga
    62221 ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt
    62281 cgattctttc cacgggtatc gctctgctaa ctctggcgag cagtttgcgc gacgctgagg
    62341 ctatcaatat tgccggatcg ctgcgtatgc agagttaccg cctgggctac gacttgcaaa
    62401 gtggcagtcc acaactcaat gcacatcgcc agttgtttca gcaggcactg cattcaccgg
    62461 tattaaccaa cctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc tatgcgcatc
    62521 tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgatttg ccgtggtatc
    62581 aggccaatat taataattat gttaatcaga tagacctgtt cgtactggct ttacagcact
    62641 acgctgaacg caaaatgctg ctggtggtgg cgatttccct ggctggcggc atcggtattt
    62701 tcacgctggt cttttttact ttgcgccgca tacgccatca ggtggttgcc ccgctgaatc
    62761 agctggttac cgccagtcag cgtattgaac acgggcagtt cgactcgccg ccgctggata
    62821 ccaacctgcc gaatgagctt ggtctgcttg caaaaacctt taaccagatg tcgagcgagc
    62881 tgcataaatt gtaccgttcg ctggaagcgt cagtagaaga aaagacccgc gatctccacg
    62941 aggccaagcg tcgcctggag gtgttgtatc agtgttcgca ggcgctgaac accagccaga
    63001 ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa gcggctgaat
    63061 atctggagtt aaatgtcggt gaaaactggc ggattagcga agggcaacca aacccggaat
    63121 tgccgatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa ctgcactggc
    63181 aaaatagtca cgtttcatca tcagaaccgc tgcttaacag cgtttcgtcg atgctgggac
    63241 gcggtttgta ctttaatcag gcgcagaagc attttcagca attattgttg atggaagaac
    63301 gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtactttct tacttacgta
    63361 tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca
    63421 tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta
    63481 ctttccgcct gacgctgcag caggcggatc tcccctccgc attgagggaa atgctggata
    63541 cgttacaaaa tcaaaccagc gccaaactga ccctcgactg ccgtctgcca accctggcac
    63601 tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga
    63661 tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg gacggcaatc
    63721 acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa cccgaaggtc
    63781 attatggtct gaatatcatg cgcgaacgcg cggaacggct aggtgggacg ctgacttttt
    63841 cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctctgcg gagggtgagg
    63901 aaagtcagtt aatgtaatgc ctcctactga ccaaagaata cttgcactta aggttcagta
    63961 taaaagggca tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg
    64021 ccgctacagt gaagcaagtc aagcctacaa cgatacgcag aaacacgagg tcctctttta
    64081 atggcgaatt tctttattga tcgccccatt tttgcctggg tgctggcaat cctgttgtgt
    64141 ctgacaggta ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca
    64201 ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc
    64261 gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctcatgta tatgtcatct
    64321 cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg
    64381 gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag
    64441 gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt
    64501 gccttcgtct ctaccgatgg ttcgatggat aaacaggata ttgctgatta tgttgccagt
    64561 aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg
    64621 caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct
    64681 aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt
    64741 ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg
    64801 caaacaccag aacagttccg cgatatcacc ttgcgggtca atcaggacgg ctcagaggta
    64861 aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc
    64921 ttcaatggta agccagcctc cgggctgggg gtaaaactgg cctccggcgc taacgaaatg
    64981 gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcatgga
    65041 ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg
    65101 gtgaaaacgc tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa
    65161 aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc
    65221 tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg
    65281 gcgatcggtc tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga acgtattatg
    65341 agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg
    65401 gcactggtcg ggattgcgat ggttctttcg gcggtatttg taccaatggc cttcttcggc
    65461 ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg
    65521 tcagtactgg tagcgatgat cctcactccg gctctgtgtg ccacactact taagccactg
    65581 aaaaaaggtg agcatcatgg gcaaaaaggc ttttttgcct ggtttaacca gatgtttaac
    65641 cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg
    65701 attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg
    65761 tcgttcttac cgctggaaga ccgtggcatg tttactacct cggtacagtt gcccagcggt
    65821 tcaacgcaac aacagaccct gaaagtcgtt gagcaaatcg agaaatacta cttcacccat
    65881 gaaaaagaca acatcatgtc ggtgtttgcc accgttggtt ctggccctgg gggtaacggg
    65941 caaaacgtgg cgcgaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc
    66001 ggcacctcgt ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct
    66061 cgcgttatcg ccagcagccc gccagcaatt agcggtcttg gtagttctgc aggttttgat
    66121 atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc acgtaatcag
    66181 ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgac
    66241 gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgct
    66301 atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt
    66361 atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg
    66421 ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct
    66481 gctttcgcga cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta taacggttat
    66541 tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat
    66601 attatggaat cgttagtgaa gcagctgcca aacggctttg gtctggagtg gacggcgatg
    66661 tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg
    66721 gtggtattcc tgtgtctggc tgcgttgtat gaaagctggt cggtgccgtt ctcggtaatg
    66781 ctggtcgtgc cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa
    66841 aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc
    66901 atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccacgacct gtttgaagcg
    66961 acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc
    67021 ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg
    67081 ggtactggcg taatgggcgg gatgatttcg gccactattc tggctattta cttcgtgccg
    67141 ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata
    67201 aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc
    67261 gggttcttgc tgtttgtact ttgtctcagg aattacttac gaagcataac ttcgataaag
    67321 tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt
    67381 ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc
    67441 gggggacaaa tcttatgtaa atactatggt cctgcggtga tgatttgtat gtgatacaca
    67501 gcaacatttc gagatattca tacggcatct aatactgatt taattctggt taaaata
//
LOCUS       DAZFZC010000013        65902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFZC010000013 DAZFZC010000000
VERSION     DAZFZC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.013"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(335..1321)
                     /gene="yqjG"
                     /locus_tag="ACHKXP_001003"
     CDS             complement(335..1321)
                     /gene="yqjG"
                     /locus_tag="ACHKXP_001003"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO7646061.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQNEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQEAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1391..1783)
                     /gene="yqjF"
                     /locus_tag="ACHKXP_001004"
     CDS             complement(1391..1783)
                     /gene="yqjF"
                     /locus_tag="ACHKXP_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7646062.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(1969..2268)
                     /gene="yqjK"
                     /locus_tag="ACHKXP_001005"
     CDS             complement(1969..2268)
                     /gene="yqjK"
                     /locus_tag="ACHKXP_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO7646063.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLETTGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2258..2662)
                     /gene="yqjE"
                     /locus_tag="ACHKXP_001006"
     CDS             complement(2258..2662)
                     /gene="yqjE"
                     /locus_tag="ACHKXP_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7646064.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(2665..2970)
                     /gene="yqjD"
                     /locus_tag="ACHKXP_001007"
     CDS             complement(2665..2970)
                     /gene="yqjD"
                     /locus_tag="ACHKXP_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO7646065.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3008..3376)
                     /gene="yqjC"
                     /locus_tag="ACHKXP_001008"
     CDS             complement(3008..3376)
                     /gene="yqjC"
                     /locus_tag="ACHKXP_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO7646066.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3523..3906)
                     /gene="mzrA"
                     /locus_tag="ACHKXP_001009"
     CDS             complement(3523..3906)
                     /gene="mzrA"
                     /locus_tag="ACHKXP_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO7646067.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(3910..4572)
                     /gene="yqjA"
                     /locus_tag="ACHKXP_001010"
     CDS             complement(3910..4572)
                     /gene="yqjA"
                     /locus_tag="ACHKXP_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO7646068.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(4594..4677)
                     /gene="yqiM"
                     /locus_tag="ACHKXP_001011"
     CDS             complement(4594..4677)
                     /gene="yqiM"
                     /locus_tag="ACHKXP_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO7646069.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(4917..5693)
                     /gene="exuR"
                     /locus_tag="ACHKXP_001012"
     CDS             complement(4917..5693)
                     /gene="exuR"
                     /locus_tag="ACHKXP_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO7646070.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(5823..7121)
                     /gene="exuT"
                     /locus_tag="ACHKXP_001013"
     CDS             complement(5823..7121)
                     /gene="exuT"
                     /locus_tag="ACHKXP_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO7646071.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7604..9016
                     /gene="uxaC"
                     /locus_tag="ACHKXP_001014"
     CDS             7604..9016
                     /gene="uxaC"
                     /locus_tag="ACHKXP_001014"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO7646072.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9031..10518
                     /gene="uxaA"
                     /locus_tag="ACHKXP_001015"
     CDS             9031..10518
                     /gene="uxaA"
                     /locus_tag="ACHKXP_001015"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO7646073.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVRLRQDVARGHKF
                     ALTNIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10601..11152
                     /gene="ygjV"
                     /locus_tag="ACHKXP_001016"
     CDS             10601..11152
                     /gene="ygjV"
                     /locus_tag="ACHKXP_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO7646074.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11157..12401)
                     /gene="sstT"
                     /locus_tag="ACHKXP_001017"
     CDS             complement(11157..12401)
                     /gene="sstT"
                     /locus_tag="ACHKXP_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO7646075.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDGRLANSALRN"
     gene            complement(13017..13982)
                     /gene="alx"
                     /locus_tag="ACHKXP_001018"
     CDS             complement(13017..13982)
                     /gene="alx"
                     /locus_tag="ACHKXP_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO7646076.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14265..15251)
                     /gene="ygjR"
                     /locus_tag="ACHKXP_001019"
     CDS             complement(14265..15251)
                     /gene="ygjR"
                     /locus_tag="ACHKXP_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7646077.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKINVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAQGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15330..16022)
                     /gene="ygjQ"
                     /locus_tag="ACHKXP_001020"
     CDS             complement(15330..16022)
                     /gene="ygjQ"
                     /locus_tag="ACHKXP_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO7646078.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATILIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAQK"
     gene            complement(16099..16602)
                     /gene="ygjP"
                     /locus_tag="ACHKXP_001021"
     CDS             complement(16099..16602)
                     /gene="ygjP"
                     /locus_tag="ACHKXP_001021"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO7646079.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKVEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16687..17823
                     /gene="rlmG"
                     /locus_tag="ACHKXP_001022"
     CDS             16687..17823
                     /gene="rlmG"
                     /locus_tag="ACHKXP_001022"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO7646080.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPQLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18107..18421
                     /gene="higB"
                     /locus_tag="ACHKXP_001023"
     CDS             18107..18421
                     /gene="higB"
                     /locus_tag="ACHKXP_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO7646081.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            18418..18834
                     /gene="higA"
                     /locus_tag="ACHKXP_001024"
     CDS             18418..18834
                     /gene="higA"
                     /locus_tag="ACHKXP_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO7646082.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNA
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18879..20897)
                     /gene="fadH"
                     /locus_tag="ACHKXP_001025"
     CDS             complement(18879..20897)
                     /gene="fadH"
                     /locus_tag="ACHKXP_001025"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO7646083.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDAGMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRTPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(21323..23674)
                     /gene="ygjK"
                     /locus_tag="ACHKXP_001026"
     CDS             complement(21323..23674)
                     /gene="ygjK"
                     /locus_tag="ACHKXP_001026"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO7646084.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLFMLYNDFFRKQ"
     gene            complement(23691..24761)
                     /gene="ygjJ"
                     /locus_tag="ACHKXP_001027"
     CDS             complement(23691..24761)
                     /gene="ygjJ"
                     /locus_tag="ACHKXP_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO7646085.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSIAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(24895..26328)
                     /gene="ygjI"
                     /locus_tag="ACHKXP_001028"
     CDS             complement(24895..26328)
                     /gene="ygjI"
                     /locus_tag="ACHKXP_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7646086.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYINGLTAEERHIEATPASNVD"
     gene            complement(26391..26840)
                     /gene="ebgC"
                     /locus_tag="ACHKXP_001029"
     CDS             complement(26391..26840)
                     /gene="ebgC"
                     /locus_tag="ACHKXP_001029"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO7646087.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(26837..29929)
                     /gene="ebgA"
                     /locus_tag="ACHKXP_001030"
     CDS             complement(26837..29929)
                     /gene="ebgA"
                     /locus_tag="ACHKXP_001030"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO7646088.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(30113..31096)
                     /gene="ebgR"
                     /locus_tag="ACHKXP_001031"
     CDS             complement(30113..31096)
                     /gene="ebgR"
                     /locus_tag="ACHKXP_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO7646089.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            31315..31647
                     /gene="ygjH"
                     /locus_tag="ACHKXP_001032"
     CDS             31315..31647
                     /gene="ygjH"
                     /locus_tag="ACHKXP_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO7646090.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGQKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(31689..33068)
                     /gene="ygjG"
                     /locus_tag="ACHKXP_001033"
     CDS             complement(31689..33068)
                     /gene="ygjG"
                     /locus_tag="ACHKXP_001033"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO7646091.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGEEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            33486..35006
                     /gene="aer"
                     /locus_tag="ACHKXP_001034"
     CDS             33486..35006
                     /gene="aer"
                     /locus_tag="ACHKXP_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO7646092.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(35160..35783)
                     /gene="nfeR"
                     /locus_tag="ACHKXP_001035"
                     /gene_synonym="yqjI"
     CDS             complement(35160..35783)
                     /gene="nfeR"
                     /locus_tag="ACHKXP_001035"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO7646093.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            36071..36835
                     /gene="nfeF"
                     /locus_tag="ACHKXP_001036"
                     /gene_synonym="yqjH"
     CDS             36071..36835
                     /gene="nfeF"
                     /locus_tag="ACHKXP_001036"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO7646094.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(36889..36964)
                     /locus_tag="ACHKXP_001037"
     tRNA            complement(36889..36964)
                     /locus_tag="ACHKXP_001037"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36929..36931),aa:Ile,seq:cat)
     gene            37089..37595
                     /gene="mug"
                     /locus_tag="ACHKXP_001038"
     CDS             37089..37595
                     /gene="mug"
                     /locus_tag="ACHKXP_001038"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO7646095.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(37674..39515)
                     /gene="rpoD"
                     /locus_tag="ACHKXP_001039"
     CDS             complement(37674..39515)
                     /gene="rpoD"
                     /locus_tag="ACHKXP_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO7646096.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(39710..41455)
                     /gene="dnaG"
                     /locus_tag="ACHKXP_001040"
     CDS             complement(39710..41455)
                     /gene="dnaG"
                     /locus_tag="ACHKXP_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO7646097.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(41566..41781)
                     /gene="rpsU"
                     /locus_tag="ACHKXP_001041"
     CDS             complement(41566..41781)
                     /gene="rpsU"
                     /locus_tag="ACHKXP_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO7646098.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            42019..43032
                     /gene="tsaD"
                     /locus_tag="ACHKXP_001042"
     CDS             42019..43032
                     /gene="tsaD"
                     /locus_tag="ACHKXP_001042"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO7646099.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(43075..44538)
                     /gene="ttdT"
                     /locus_tag="ACHKXP_001043"
     CDS             complement(43075..44538)
                     /gene="ttdT"
                     /locus_tag="ACHKXP_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO7646100.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIAVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(44586..45191)
                     /gene="ttdB"
                     /locus_tag="ACHKXP_001044"
     CDS             complement(44586..45191)
                     /gene="ttdB"
                     /locus_tag="ACHKXP_001044"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO7646101.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(45188..46099)
                     /gene="ttdA"
                     /locus_tag="ACHKXP_001045"
     CDS             complement(45188..46099)
                     /gene="ttdA"
                     /locus_tag="ACHKXP_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO7646102.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            46306..47238
                     /gene="ttdR"
                     /locus_tag="ACHKXP_001046"
                     /gene_synonym="dan"
     CDS             46306..47238
                     /gene="ttdR"
                     /locus_tag="ACHKXP_001046"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO7646103.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(47251..47868)
                     /gene="plsY"
                     /locus_tag="ACHKXP_001047"
     CDS             complement(47251..47868)
                     /gene="plsY"
                     /locus_tag="ACHKXP_001047"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO7646104.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            47973..48341
                     /gene="folB"
                     /locus_tag="ACHKXP_001048"
     CDS             47973..48341
                     /gene="folB"
                     /locus_tag="ACHKXP_001048"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO7646105.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            48431..49252
                     /gene="bacA"
                     /locus_tag="ACHKXP_001049"
     CDS             48431..49252
                     /gene="bacA"
                     /locus_tag="ACHKXP_001049"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7646106.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(49433..50671)
                     /gene="cca"
                     /locus_tag="ACHKXP_001050"
     CDS             complement(49433..50671)
                     /gene="cca"
                     /locus_tag="ACHKXP_001050"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO7646107.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(50735..51355)
                     /gene="ygiM"
                     /locus_tag="ACHKXP_001051"
     CDS             complement(50735..51355)
                     /gene="ygiM"
                     /locus_tag="ACHKXP_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO7646108.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            51597..52898
                     /gene="ygiF"
                     /locus_tag="ACHKXP_001052"
     CDS             51597..52898
                     /gene="ygiF"
                     /locus_tag="ACHKXP_001052"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO7646109.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            52921..55761
                     /gene="glnE"
                     /locus_tag="ACHKXP_001053"
     CDS             52921..55761
                     /gene="glnE"
                     /locus_tag="ACHKXP_001053"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO7646110.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALCNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLSYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            55809..57242
                     /gene="hldE"
                     /locus_tag="ACHKXP_001054"
     CDS             55809..57242
                     /gene="hldE"
                     /locus_tag="ACHKXP_001054"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO7646111.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(57362..57421)
                     /gene="ibsE"
                     /locus_tag="ACHKXP_001055"
     CDS             complement(57362..57421)
                     /gene="ibsE"
                     /locus_tag="ACHKXP_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO7646112.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(57737..57796)
                     /locus_tag="ACHKXP_001056"
     CDS             complement(57737..57796)
                     /locus_tag="ACHKXP_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7646113.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(58036..59697)
                     /gene="yqiK"
                     /locus_tag="ACHKXP_001057"
     CDS             complement(58036..59697)
                     /gene="yqiK"
                     /locus_tag="ACHKXP_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7646114.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVEEKAE"
     gene            complement(59724..60353)
                     /gene="yqiJ"
                     /locus_tag="ACHKXP_001058"
     CDS             complement(59724..60353)
                     /gene="yqiJ"
                     /locus_tag="ACHKXP_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7646115.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            60622..60822
                     /gene="glgS"
                     /locus_tag="ACHKXP_001059"
     CDS             60622..60822
                     /gene="glgS"
                     /locus_tag="ACHKXP_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO7646116.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(60865..61929)
                     /gene="yqiI"
                     /locus_tag="ACHKXP_001060"
     CDS             complement(60865..61929)
                     /gene="yqiI"
                     /locus_tag="ACHKXP_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7646117.1"
                     /translation="MRYLLIVITFFMGFSSLPAWAMDCYAEHEGGNTVVIGYVPRISI
                     PSDGKKGDKIWQSSEYFMNVFCNNALPGPSPGEEYPSAWANIMMLLASGQDFYNQNSY
                     TFGVTYNGVDYDSTSPLPIAAPVCIDIKGAGTFGNGYKKPAVCSGGPEPQLSVTFPVR
                     VQLYIKLAKNANKVNKKLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVDLEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDIGNGLLLKIFHNDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(61931..62679)
                     /gene="yqiH"
                     /locus_tag="ACHKXP_001061"
                     /pseudo
     CDS             complement(61931..62679)
                     /gene="yqiH"
                     /locus_tag="ACHKXP_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(62695..65199)
                     /locus_tag="ACHKXP_001062"
     CDS             complement(62695..65199)
                     /locus_tag="ACHKXP_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7646118.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLINNQTAVCVTPEQLTL
                     LGFTDEFIEKTQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWNHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQATNLDFPRIYLFRPIPAMNAKLTIGQYDTESSIFDSFHFSGISLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGRVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNEISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GTEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIIGVIRLADGSHPPLGISVKDETSHKELGLVADGGFVYLNGIQD
                     DNKLALRWGDKSCFIQPPNSSNLTTGTAILPCISQN"
     gene            complement(65259..65810)
                     /gene="ygiL"
                     /locus_tag="ACHKXP_001063"
     CDS             complement(65259..65810)
                     /gene="ygiL"
                     /locus_tag="ACHKXP_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO7646119.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQSDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT        15880 a        16708 c        17218 g        16096 t
ORIGIN      
        1 aaaccgacat aattttttag tactttcaga taccagtcca tttttgcctc aattttgctc
       61 aatcacttgt ttttaaagca gcaaattaag aatagacgtg aatgaaatgg cagtaaatat
      121 tcgttcaagg aattaataaa gtgccgtgaa ggcgcgctaa gactgatgac aagcgcaaaa
      181 tcgccagatg cgctacgctt atctggtctg cgcgggttat gcaatctatt gaatgtacaa
      241 tgttttgtat gtcagataag gcgttcatgc cacatccgac atgaacaaca tgcacttagc
      301 taattatcag aggcacctcg taaggtgcct ttttttaacc gaagcgaaca tctcgtccat
      361 gcggttcatc gagatcctgc cacggaccaa ttgaaataat ccccgtaggg ttgatggtct
      421 tatggctgcg gaagtaatga ttacggatat gatcgaaatt gactgtttcg gcgatccccg
      481 gcatctggta gatatcgcgc agaaaaccgt acagattcag gtaatcgctg atgcggtgct
      541 tatcacactt gaagtgggtc acatacaccg gatcaaaacg caccagcgta gtccacaggc
      601 gaatatcggc ttcggttagc tggttgccgg tcaggtaacg atgctggcct aaaatctgct
      661 ccagtcgtgc cagcgattca aacactttcg ccaccgcctc gtcgtaagct tcctggctgg
      721 tggcaaaacc agctttatac acgccgttgt taacggtgtc ataaatccag ccgttaagtt
      781 cgtcaatttt tgtttgcagg gctggagggt agtaatcacc cgctttcgcg cccagcgcat
      841 caaacgcggt attgaacatg cggatgattt ctgctgattc gttgctgacg atggtgtggt
      901 tctttttgtc ccacagcacg ggaacagtaa ctcgtccgct gtagtgtgga tcggcgtgga
      961 gataaagctg atacagaaat tcattttgat agagcgtgtc tccggttgct cccggaaaac
     1021 tgtcatcaaa ggtccaaccg ttttccagca tcagcgggtt cactacggaa acggaaataa
     1081 acggttccag tcctttgagt ttgcgcatga tcagcgtgcg gtgcgcccac gggcaggcga
     1141 gtgaaacata gagatgataa cgatctttct ctgcgataaa accgcctgtg ccagtggggc
     1201 caggagcgcc atcggcagtg agccagttac gaaatgcgga agctgaacgt tgaaatttac
     1261 cgccggtaga tttggtatcg taccaggtgt catgccagac gccgtcaatc agttgaccca
     1321 tttttctctc ctccaatagc aaaaacgagg agcttcctcc tcgtttttta tcgttaagta
     1381 tagcgcgtgc ttaccacttt ttattcagca ggcggtcgat gctatacgcg cccggaccgg
     1441 taattgccag cagcaggaat ccgccagaaa ttgtcaggtt tttcatgaac atcagcgagt
     1501 tgacgccttc agcaaagttg ctgtgaaata aaaatgccgt cagcagcgta aagcccgcag
     1561 taaacagggc tgtggtgcga gtcaggaaac cgaacaggat tgccagacca ccaccaaact
     1621 caagcagaat caccagcggc agcataaaac ccgggacgcc cattgcttcc atatattgtt
     1681 gggtacccgc gtagccagta atttttcccc agccagcggt aataaacaga atcggcatta
     1741 aaatgcgcgc taccagtaca ccaacatctt ctaatttttt catcatactc tccactgcgc
     1801 cactcaggct gctgattgtt tttttgtgca atggcgcggt attagcgtcg ttgctgtcga
     1861 tggagagaat cataaacgtg gtgaatgatg attgttagca aggaaaactg tcaaaaatct
     1921 tcaaaaaatt tgagggataa ggccggaatg gctccggcca gagggaagtt aaccgcgaag
     1981 ctgttgctgc ttgagggtcg ttttaaccag acgccaggcg ctccatacgc caaaaccgcg
     2041 tctggcccag cggaccagca tattaggatg gcgaatcgtc cagatcgcca tcacgctact
     2101 gccaaccagc gcccaggagc gcagacttag cagcatattc cagcgacgat cgtaagcgcc
     2161 tgttgtctcc agccattcac gacgactggc ggaaagatcc agccgttgtt gctggatctg
     2221 gctaagcagt tgcgccttac gtcgttcacg ttcgacttta ctgctcacgg gactcctcct
     2281 cgagcagctg ccgatcgttt gctaactcat ggcgtgtatg gcgcagcaac gtagacttac
     2341 gcgatttacg tagcgtccag ataccgccaa tcagtgccag tagcagcaac accacggtgg
     2401 tggcaatcat cgcattcagg cgatattgcg ggtcaaccgc ccaaataatt agcaccatca
     2461 ggctcataag accaaatgca gcgaaaagca tcgtcaggcc cagcatcagt aaaagttgaa
     2521 agagattcgc tttttcctct tccagctcca ccaccgccag ccgcagacgt gtctctacca
     2581 tttcaaccat gatagaaaca attcgctgcc cgatgcccag aacgctttta ccgggccctt
     2641 gtgcgtgatg agtgtccgcc ataattaacg acgcgacagc agaacgccga gcactacacc
     2701 gattgcagcg ccaatgccca cgcccgtcca cggattttcg cgcacatact catcggcacg
     2761 cgccgccgcg acacgggttt gtttggcaat ggcatcaccg gtttcaccca ggcgataacg
     2821 gctctgtttc agtgcctgct ccgctttgct acgaatctta ctcaactctt ctttcgactt
     2881 ctcgccagat gagctaagca cctcttccag cgtatcggaa agggatttca actcagcacg
     2941 cagatgttcc gtagtgtgtt ctttcgacat agttttctcc aggtgagtaa gtagtgacta
     3001 ttgtgagtta gtagtcgcgc gcttccagct ttttcagctc ttcctgcgct tctgccagtt
     3061 tccgttcgcg tttggcaatc ttatcggcat cgcctttttg cttcgcctcg gctaaatctt
     3121 gctggcgttc cgccacctca tctttctgct ttgcgatttt cttctgatga tcggcacgca
     3181 gctggctatc tgaacagttg gcccggactt cactcagggc tttattcaga ccgtcaatac
     3241 gattctggtt ttggtgtttt tcggcatagc tgatctcctt aaggatattt tgctcctttt
     3301 cctgacacag ggtagtggca taactaccgg cactaagagc aaagagagaa acagctaaag
     3361 cgatgcggta tttcattctt gaaccttcca ttgtgattcg gacctattac aaaggaacga
     3421 ttgtccagac aacgaagcgg tagtgttaac caccgcctca ttaatcatag acagttacgg
     3481 tgaaatcacc aaagtgagtg atatgtttcg gataacagga agttatccga agcgatgaga
     3541 gttatcccgt aaccgagtca gccactgcat agcctgactg ttattgtcct gctgcgcaat
     3601 gatgtagcca tggggcaatg ttctgtcgag gaccgctttt gcggcggcgc tctggtcgct
     3661 ggagtcaaat gtaattaaca gagtgtcgtt tttgggggtg atacttttga aaggaatgcc
     3721 atgagcgtca aggtgatgcc agattgaaaa accgtctggc atcgttgtgc cttgatgaac
     3781 ggcacgaatc gccagcgtag actcttgctg gcgaaccgcg gaccaggcca gcaacagcgt
     3841 gccgaccaga agtaaaacag cgccggacca ggctagctgg cgaagcgaca tgcgaggtat
     3901 ttgcatccct tacccccgat ttccatattt ctttttccat aacacgacca gagaacctgc
     3961 caggccaaac accagcagca ccaccgggag cagcatcagg catgacatca gctggtcctc
     4021 gtactttaaa aataccggcg ttttgccgag catgtaaccc agagttgtca ggatcaatac
     4081 ccacagcaga ccgctcatcc agttgaaaaa ctgaaagcgc gcgttattca gccctgataa
     4141 cccggcaatc gtcggcagca gtgttctgac aaacgcaata aagcgaccaa ttaacagcgc
     4201 cgataaaccg tgtttatgaa aaagatggtg tgcgcgttga tgataatgcg cgggtaaatg
     4261 agatagccag ttttgtacgg tgcgggtatt gcccagccat cgcccctgaa tatagctgac
     4321 ccagcagccg aggctggcgg caacggtcag cagcagaatc gtttgcggat agcccatcgc
     4381 gcctttcgca atcaacacgc cgaccaatac cagtaaactg tcgcccggta aaaaggccgc
     4441 cggaagcaag ccgttttcaa ggaacaaaat tacaaacaag acaaaataca acatgccaat
     4501 catcgatgga ttggccaggg tttcaaaatc ctgcgcccac agggcttgca gcaattgggt
     4561 caaaagttcc attcattatt cctggtgaat cggttaacgc cacgctggtt ttaaaagatg
     4621 cactaccgca atgttattgc tgttttggta tgttctgcgg gtttgataca tcaacacagc
     4681 ctgaactatt ccctgttaaa tcgtgagata agaaagcatt caaaaacata aattcaaatt
     4741 gaatctaatt gtaacaaagg tcaacgttgt tcgttatcaa aattacatgt ccgtggagtc
     4801 tgattttgct gaatgcaggg gagggtggcg agaggattta cacaggctga cactttatac
     4861 ttttcgctta ccgacgagac aaacaggcgc attgcagcgc ctgtcggaca ggaatttcat
     4921 ttactgccgc tggtggctgt atcgagatgc accaccgggt tttcggcgaa tagatagcgg
     4981 tcggcattga actcgaagtc gtcgctggtt tcgttaaata acatgatctt ggtgttttcc
     5041 agatgctgcc acattgccag cttagcagca tgaggatctt tgcgaatcag cgccttgagg
     5101 atttgatcgt ggtcatcaca ccagttatcg acggtacggg aatcaatgtg ttcgtgcagt
     5161 tttttccagt acgggttatg actacgctgg gtccacattt tttccacgat agccgccagg
     5221 gcggagttct gcgtcgccag agcgacctga atgtggaact gcaaatccca ctcggaatca
     5281 cggaagcatt gttcgccgcg cgcctgttcc tgaatggcca tcagtttcat gatgtcctgt
     5341 ttcgttacct gagttgccgc gaattcggca atattacttt cgatgagctg gcgagcctga
     5401 agcaactcaa acggaccgta attggcgaat tccatattat tgtcagccgc ctgctgatgg
     5461 cgcggctggt tggaaaccac atgaatacct gaacctttac gcacttcgac atagccttca
     5521 acttccagca tgatgatggc ttcacgaact accgtacggc tgacgttctt ttcatcggca
     5581 ataaagcgtt ctgcaggcag tttatcaccc accagataga cgccctgttc gatgcgctct
     5641 ttcaggtcag cggcaagttg ttgatacaaa cggcgtggtt cagtgatttc catatgcgct
     5701 ccaggcatga tacggcagac tatatttgtt ataccacttt tgcgagttta gctctactcg
     5761 cagtgatgaa aaagccgcct gagaaggcgg ctttgaaggg aaactaaaac ttacacgaaa
     5821 cgttaatgtt gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat cgccggtttg
     5881 ttctgcaaca cggtccagat aaccagcgca cctaacaggt cgaacactgc cagaactgcg
     5941 aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta
     6001 cttgccagcc atgcggacat cccggttaaa ccgtttgccg ttgccacttc gttacgacca
     6061 aacacatcgg aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg
     6121 atacacagca gcataattgc gacatacggg ttggtgaaca ggccgatcat acccgggcca
     6181 atcatcagca ctgcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca
     6241 aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac
     6301 agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac
     6361 atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgccag gaaacgcggc
     6421 agcgcgatac cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc
     6481 gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc
     6541 agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag
     6601 ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcattacgat tgcccatacc
     6661 accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg
     6721 atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc
     6781 gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc
     6841 agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata accgattttc
     6901 gtacccagca catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa
     6961 taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt
     7021 ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc
     7081 agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct catcgttttg
     7141 ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc
     7201 gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc
     7261 ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc
     7321 cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt aaaaggtgag
     7381 agccatcaca aatgtgggaa tatttgtagg gacattacct gacgacagca aggccagtac
     7441 tggcgcggcc tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg
     7501 atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc
     7561 gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac
     7621 tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga
     7681 ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg
     7741 ttttaaaaac ctatatgaca tctggctgaa aggcgatcac tacaaatggc gcgctatgcg
     7801 taccaacggt gtggccgagc gtctgtgtac cggtgatgcg tctgaccgtg aaaaatttga
     7861 cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcacct
     7921 cgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa ctgccgatga
     7981 aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat
     8041 gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca
     8101 tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc
     8161 ggacaaagcc ttcaacatcg aacaggcgac ctttaacgac tacatggcga agctgggcga
     8221 agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct
     8281 ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt
     8341 tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg ctggcgaaac
     8401 cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga
     8461 atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct
     8521 gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat
     8581 ggcagaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac
     8641 cattctgtac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt
     8701 ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca
     8761 gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtttgc tgagccgctt
     8821 tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg
     8881 ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat
     8941 caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc
     9001 cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat
     9061 aacgtcgcgg tcgctttagc ggatttggct gaaggcacag aagtcagtgt cgataaccag
     9121 actgttaggc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac gaatatcgca
     9181 aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg
     9241 gcgggggaac acgttcacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat
     9301 cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc
     9361 tatcgtcgcg ctaacggcga tgtcggagtg cgtaatgagc tgtggatcct gccaaccgtg
     9421 ggctgtgtca acggcatcgc gcggcagatc caaaatcgtt tcctgaaaga gaccaacaac
     9481 gccgaaggta ctgacggcgt gttcctcttc agccacacct acggctgctc acagctgggc
     9541 gacgatcaca tcaatacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc
     9601 gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg
     9661 cttggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag
     9721 atcgaagccg gaatcgagca tttgcatcag ctgtataacg tgatgcgcaa tgataaacgc
     9781 gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt
     9841 tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc
     9901 ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac
     9961 cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag
    10021 tactttattg cccatgatca gccgatctat gaaaacccat cgccggggaa caaagcgggc
    10081 ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagcgtc
    10141 gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt
    10201 gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg
    10261 ctgttcagta ctggtcgtgg cacgccatac ggcggctttg taccgacggt gaaaatcgct
    10321 accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg
    10381 atccacggca aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt
    10441 gccaacggta aacaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa
    10501 agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt
    10561 gaagcgccgt ttgcttttcc atacctgata aggatttatc atgaccgcgt attggctggc
    10621 ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga
    10681 acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct
    10741 tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac
    10801 cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg
    10861 tctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggcacga ttgtcagtac
    10921 ctgggcgctg ttccgctgta aagggctgac catgcgctgc gtaatgtggt tttcaacgtg
    10981 ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag
    11041 ttttctgctt atgaatggcc tgaatatcat ccgtttctgg cggatgcaga aaaggggaat
    11101 tgatccgttt aaagtagaga aaacccctcc cgccatagac gaaaggggtt aagcaattaa
    11161 ttacgcaggg cgctatttgc cagacgaccg tcttctgcct ggcaagctgc cgcagtgaac
    11221 agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgatgata
    11281 aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc
    11341 agtgggatca gcagcagaga cccccctgcc acgccggatg cgccacaggc acacagagaa
    11401 gccactacgc tcaacagcag cgctgtgggc agatcgaccg gaatacccag cgtattaacc
    11461 gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggcaccc
    11521 agcggaatag aaacggaata ggtatcgcga tccagattca gcttttcaca cagcgccata
    11581 ttcaccggaa tgttagctgc agagctgcgg gtgaagaagg catacacgcc gctttcgcgc
    11641 aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg
    11701 ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag
    11761 ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagccc aaaaataccg
    11821 atcggtgcga agcgaatgac cagtttcacc ataaaggtga cggcattcga catatcgttg
    11881 accaggtttt tggtggtctc gttaccgtga cgcagtgcga agccgaggcc aattgcccac
    11941 accaggatcc cgatgtagtt acctttcagc agcgcgtcaa tggggttgga aaccatgctc
    12001 attaccagtc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta
    12061 ctggataagt gcagggtaga agggaaggcg aagctgaaaa cgactgcggc cagagcagca
    12121 gagaaggtac ccagcagata gaggaacaaa atagggcgga tattggtttt ctgcccgtgc
    12181 tggtggttag caatagatgc catcaccagc atcaacacca ggatgggggc aacggctttc
    12241 agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc
    12301 catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga
    12361 gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt
    12421 gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt
    12481 gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag
    12541 agaggtcatg ttttgcaact taattgttac atttgtttga cataaacaga gggaaggact
    12601 ggctactaag gctgattaga taacatatgg gcaaaactat ttggaatatg agaacggttg
    12661 cgctccttta atgtgctatg ccatataaag tcactggcgc agggagcgcg cagggggcgg
    12721 ccaatcgccg ccgccccctg cacccccagg ctctggcgaa caaaatcgcc gctgcgcgat
    12781 tccctcagct tatcccttgc ggctaccggg tcgggcgcaa gggaacgtcc ctgtaagatg
    12841 cgccctcgcc gcgcatccat gcgcgtcgcc ccggcctcca gggaacgctt cggcgatttt
    12901 gacgccacca acacccgtgc ggtctttttg taaagagcta caagccgatt acataattaa
    12961 tagctcacct ttgtctggaa tgagttatct ctaggaatgg cttaatatac agcgaatcat
    13021 tctccccgct gcttatcatg ccgataattc acccaggcgt tgataataaa cgtcatcacc
    13081 agaatgccaa acaccacgcc cagcgagacg gcgattggaa tatggtagaa gtcgacaatc
    13141 agcatcttga taccgataaa caccagaatc accgccaggc catatttgag catcgagaaa
    13201 cgctctgcta cgcccgccag caggaaatac atcgcacgca ggccgaggat cgcaaacagg
    13261 tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga agatagccgg aatgctatcc
    13321 acggcgaaaa tcacgtcgct caattccacc agaatcagta ccagcatcag cggtgtggcg
    13381 tacagcaatc cattcttacg cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc
    13441 aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa tgcctgattc atcttcatgg
    13501 gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac cgaagatata caggatccag
    13561 tcgaactgcg aaattagcca gctgccagtg aagatcatga tggtacgcag aacaatcgcc
    13621 ccgagcacgc catacaccag cacgcggcgt tgtaatgccg ccggaacaga gaaatagctg
    13681 aacaacatca gccagacaaa gacgttatcg accgccagcg atttctcaat cagataacct
    13741 gtgagaaagg ccagtgcctg tggatcggca acggcgcgac cttcggtttg caccagatac
    13801 caccagaaag cggcgttaaa cagtaacgac agcgtcaccc agaccagcga ccaggccgca
    13861 gcctgtttca tggtcatggc atgtgcccca cgacgcccct gcaacaacag gtcgatagcc
    13921 agcataatgg cgacaacaac agcgaatccg ccccatagca acggcgtgcc gacagtattc
    13981 atagaagttc cttacacata aaaaaacggc caacgtcgga agacgtcagc cgctgctttt
    14041 tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga atacgtctgc
    14101 gtattccttt cgggttgccc ggtgaccgga tgtggttttt cacacatcgt aatgacgatc
    14161 gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc attcagccag
    14221 tcaatggtct gttacatacc ttttacactg ttttactttg gcaattatag ttttacgcta
    14281 tctgccggaa aaatcacccc agtctggcgg cggatctcgg tcagcagttt ggcggtgatg
    14341 cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt agcgaacagc
    14401 tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg catttggctg
    14461 ccacgcggca cgaagcacac tttctggcat tcagacagtt tttcaatcac cagcgatcct
    14521 gcttcgccct gaatctcgct cgccaggaca gaatcactga cttttgagtg ctgcaaggtg
    14581 acgctgaaat caccgtaatc catcaccacc acgccctggg cgtcaacgcc gcttgccagc
    14641 aaactggcgg ttgcctgcac gcttttcggc tcaccaaata acgccaccgc cgacgccaga
    14701 cagtaaaaac cgatatccat aattgaaccg ttagagaatg ccggattaaa ggtgttggga
    14761 ttctcaccat ccaggtaacg ttgataccgc gaggaatact ggcaatagtt gaaaaagact
    14821 ttccgcagtt tgccgacttt cggcagcgcc tggcgcaaca aatgaaagtt cggcaggcag
    14881 gcggttttaa atgcctcaaa cagcaccacc tgattttccc gcgcacaggc aatggcggca
    14941 tccacttccg ccagattcga cgccagcggt ttctcgcaaa tcacattaat tttatggcta
    15001 aggaaaagtt gtgtctggga aaaatgcagg gaattcgggc tggcaatata caccgcgtca
    15061 atggcatcgc tttccgccat cgcttccagc gaggtaaaca gatgctcgac agaaaaatca
    15121 ttggcgaagt gctgggcctg ttcaaggctg cgggaatata cggcggttaa cttgtattta
    15181 ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc aatcacagcg
    15241 aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg tagcacgcct
    15301 ttttcatctt cgacgtgcgc agatacgcat tatttctgcg ccggacagcc atgctcacta
    15361 aacggcccaa tcatgaccga aggcccgaga tatttcggct ggcgatggag gattttggcg
    15421 tcaatcacgg cgctgacacg agcgagtttc tcccgcagtt gagtataaaa accgtgcttc
    15481 atattgaggt ctggcgcgtt aaaaccgata gcgtcgatgc cgtactgttt tgccagccag
    15541 atggcgcgtt gattatgaaa ttcttgcgag ataatggtga tatggttttc accaaaaacc
    15601 tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca gaagatcact
    15661 tttgctggta cgcctttagc gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt
    15721 ttacgtccgt tatcgccact taccagcagc catttcactt ttccggcgtg gtacaacgct
    15781 gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctcgcccc cagcaataaa
    15841 cccacgttac gcgccggaac ggcgttgaca tcgctccagg tcagctgttt gctggcgttg
    15901 accattacgc gatcggcgat aaggatggtc gctcctgcca caagtaacag taagcaggca
    15961 atctttagag tccggcgaga aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc
    16021 atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag aaagaaaaga
    16081 taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag
    16141 tcgggtgtcg aactcaagct ggtggtactg gggttccata tgacaacaca actggtaaaa
    16201 cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa
    16261 aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctccacct ttgctttcag
    16321 cttgccgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg
    16381 gatcttattg tcatacatca ctttattgat cggcggggca ttacgcagaa actgattttt
    16441 cagatcctga gtatattgcc agagggcttt atcggtggcg tagtcgtgtg ttcccggata
    16501 gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agtgttcgca cctgggaaag
    16561 tagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga
    16621 gaaaaagggt atactcacgc acccttttca ggggcaatgt cgaaatttta tcattcagga
    16681 gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg
    16741 acggatgacg ttaacccgct acaggcgtgg gaagcggcgg atgaatattt gctgcaacag
    16801 ttggacgaca cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta
    16861 agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat cagtgaactg
    16921 gcgacgcgcg agaatttacg cctcaacggg attgatgagt cgagcgtgaa gtttctcgac
    16981 agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg
    17041 gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt
    17101 attgccggtg ccaaggcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg
    17161 ctcggcccaa ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcactttc
    17221 aatgaaccgc agctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac
    17281 tggactatcc acaaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc
    17341 ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt
    17401 aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc
    17461 gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag
    17521 gcgttggatc gctgcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc
    17581 tttaatgctg tgctctgcaa cccgccgttt caccaacaac atgcgctgac cgataacgtc
    17641 gcctgggaga tgttccacca cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc
    17701 gttgccaacc gtcacctgga ttacttccat aaactgaaga agattttcgg caactgcacc
    17761 actattgcga cgaataataa atttgtggta ctgaaagcgg tgaagctggg acgtcgtcgg
    17821 taagattggg agttacctgc cggatgcggc gtaaacgcct tatccggcct acgttcagca
    17881 cgttactcca ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc
    17941 tggaatcgcg cttccataaa acaaaaaatc cctgtcacca tcgtctacat tctcttgttt
    18001 agcgtttttc tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctggc
    18061 aacggatgtt atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac
    18121 tcaaaaagca ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc
    18181 tctggggaac acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt
    18241 cccatctctg gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa
    18301 tgaattacgt gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt
    18361 tatgacgcat aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg
    18421 attgcgattg ccgacatctt gcaagcagga gaaaagctaa ctgcggtggc accttttctg
    18481 gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg
    18541 ctcaacgctc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa
    18601 gaatcagcgc ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata
    18661 gccgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct gccggaaatt
    18721 ggcagtaaat ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac
    18781 gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac
    18841 gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag cgccagccgt
    18901 gttccctggg caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt
    18961 aaatgcaccg ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc
    19021 cctgcgcaga tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca
    19081 tgcagcccgt catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc
    19141 gagagcaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcctggtttg
    19201 ctggctttgc gctggagcat cacaatctgc cgtgggctac gggggatctg cattccctgc
    19261 gggcttaagc caccagcctg ttgtaggcta ctgtcgatcc cccattcatt acagaacccg
    19321 gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca
    19381 atcccgccac aaccgatgat ggcaactttg ttgccaaccg gcgctttgtc gcgcagtaca
    19441 tcgagataac tcaatacctt cggatgatcg atcccgtcga tgggcggagt gcgcggcacg
    19501 atcccactgg cgaggatcgt ttcatcgaaa gcctgtaact gatccgccgt cacggtgtga
    19561 ttgagtttta gcgtcacgcc cgtcacttcg atcatccggc ggtaatagcg cagcgtttcg
    19621 taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc aatctcgcta
    19681 tgagcgtcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc
    19741 ccagcaggtc ccgcaccgac caccgccaga ttttttttct gcacggcggg aaggattggc
    19801 attttggttt cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag
    19861 atttgatcga gacaggcctg attgcagcca atacaagtgt tgatctcatc ggctcgtccc
    19921 gattgcgctt ttgacagcag ctccgcatca gcaagaaacg gtcgcgccat cgataccata
    19981 ccggcatcgc cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg
    20041 gttaccagcg gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg
    20101 cgcggcacgg gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg
    20161 ataatggtcg cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc
    20221 ccgccgtctt cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg
    20281 acgcgttcgc gcaccgcacg cactacttct acggcaaatc gcatccggtt gcggtaatcg
    20341 ccgccccact ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac
    20401 ccttcggaac ccatcacctc tacaccgtcg tatcctgcct cccgcgccag ttgcgcgcag
    20461 cgggcgaaat tgtcgatcag ttgcaggatc tcttcatggc ttaactcatg gggcacgaaa
    20521 cggttgatgg gggcctgcaa tgcggacggg gcgaccagat gcggttggta gctgtagcgc
    20581 ccggtatgca aaatttgcag ggctattttg ccgccttcct gatgtacggc ttcggtaatg
    20641 gtgcgatggt gtgggatctg gctggcgtca ttgagcattg caccgccttc catgccaacg
    20701 cctgttaaat ctggtgcaat gccgccgctg acaatcaacg ccacgccgtg acgggcgcgt
    20761 tcggcataaa acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc
    20821 attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac
    20881 agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga
    20941 gttctaattt agccgtcgcc ggagaaaagg gaaaagggaa gtgcggggtt tgtgatggga
    21001 ttcaaaaaga atacgcccgg tggtaacgcc ggatgcgacg cttgccgtgt tttatccggc
    21061 ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg
    21121 tgcagatgcc ggatgcgacg cttgtcgcgt cttatccgac ctacgggaac gcacatgtag
    21181 ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg tgcagttgcc ggatgcaacg
    21241 cttgccgcgt tttatccggc caggaggatt gcacatgtag ggcggataag gcgtttacgc
    21301 cgcatccgcc agcagttgca aattattgct tacggaaaaa atcgttatac aacataaaca
    21361 aatgcgcggc actccaggag aaattcggtg ccccttgctg cgcgccagtg agcgggttgt
    21421 aattctcctg aattggtcca tcggcggtta accctttggc gtgccggaag aacgtatccg
    21481 ccagcttcag ggcatcatcg cgataaccgt aacgctccat ccctttcaga ccaaaccaga
    21541 actgatccac ccatacgcgc ccgcgccagt agatatccgc gccaaaagcc gggttggtta
    21601 acgccgccgt tcccagcggg acaaaggtgt tgaactcttt aggatcgagc atcaccttca
    21661 ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa cagcggcgac cagccttccg
    21721 gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagc ggtttatctt
    21781 caatacgcac gtcatagtag aactgggtag tcgggtcgaa catacaggtg ttgatgtagt
    21841 ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc ttctggctta ccgaggatgg
    21901 ttgccatctc cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact
    21961 cctgcaatag cgagtagccc agcaacgttc cgtcctgact gcggttttcg gcgaatttca
    22021 ccgtccagtc gctacgtttg ccgccattag cgacatattt atccaactgt tctttgtcga
    22081 taaacccaaa gacggcggca tcatcacgcc ccgattccca cgatgcagca acctgtgccg
    22141 gaatttccag actgtcgtac tggcctttct ccaccacgcg ggcgtagttg ttcagcccag
    22201 actgcgtctc ttctttatcg ccttttttca ccgtaaacag catctcgccg ctctcagtgt
    22261 tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc gttgccgttg tgatcgcggt
    22321 tacgtaacca ccagtcgtga taggccacca gtttcgggta catctctgcc acccaggttt
    22381 tatcttgggt gacgttatac acttccatca ccgaccaggc ggcaaggctg ggtttggtat
    22441 tacgttcgtt ccagttgccg ccatcgccac cacgctcggg gctaaggttc cacgctatca
    22501 ggtcggggac aaagcccaca tcctgcggac gcacgctatc gccaggctgg atctgccagg
    22561 agaagaccgc gcggatattc tctttggcga tgtccggatt gaaatgcgcc atcgcaaacg
    22621 cctgcttcca ggtatcccac ggccaggtct gattgccgga gaaccagcgc ccggtcaccg
    22681 acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga gcgccagtta ccgttgagcg
    22741 tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc
    22801 ctttcttcag gtattcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg
    22861 ccagaatatc gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcagatggg
    22921 aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta aagcgattac
    22981 cgttgatttc agtctgcacc ggcagggatt tatgcacctg atattccgat tcgccggagg
    23041 tcagcagatc ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg
    23101 cgctgatttt gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggtt
    23161 tcccttcttt cgcttccagt ttttccagca gttcgccgtc ccacaccaga tccagcggtt
    23221 tattgctggt gattttggtt tccagtagtg acgtgcgcgg cgtggcgaag cgcagagtca
    23281 tttcgacttg cacatctttt gctgtcagtt tttgcaccag cgcaccggga atactgtatg
    23341 cctccagcgt aaagtcgact ttcttgccgt cctgccagac ggtcaggcgg tcgaaattgc
    23401 tggccataaa gttgatgtac tcttccgtca gcagcgcaac gcccggaaag ccgcccatgg
    23461 tgttagggcc gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa
    23521 aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca gtacggttaa
    23581 tcacgttttt ataattgtcg gcgttagcgg catgggcgga aaaacttatc agcagagcgc
    23641 aggttactgg cgttaaaata gttttgattt tcatgaaatt acagtcctta ttaccagtag
    23701 aaatattgca tcaggaagat ttggtcgctg ggatcgtgag aggcattttt catgtagaag
    23761 cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt cgtaccagat accaaactgc
    23821 atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg
    23881 atggagagat aaacgccctt taacgcttca tacatggcgc tcaccatcat cccactttca
    23941 cttcccggta cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg agcggcaaag
    24001 ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc cgtcggtgat ttccagaccg
    24061 ttcatccagg tgatgccttc ggcaaggtca tactggatgt aaccgttcat cattgccgga
    24121 taggtgtatt tgtcgtcata gcgatcgtat ttgccaaaat gtagccaggc tttgctttca
    24181 tcatgatgtc cggcaggagt cgcggtgacg ctataacgaa agttgccgct taagttctgt
    24241 accttcagcg cagtcataat gtcgcgggta ttgggaatga cgtagcccag atcgggggtg
    24301 aaatcgcccc accattgcag gtcatccagc gaggaatcct gacgcagact gagcatgatt
    24361 tcggtgccat cattggtacg ataaccgcca tagaaacggt taattccgcc ctcgaaacca
    24421 ccccagctat ggtctggcac ccaggcattg ccgtcgtggt cggcctgcac ggtccagcct
    24481 tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct ccaggccacc ttcaatatag
    24541 gtaccgtcgc tgtatttatg tttattctca ccctctaaat ccatatatcc accaacccct
    24601 aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg taattgcagg tgtttttgtg
    24661 tcgtcatgtt ctgccggacg tgcttcttct cctgccgcag aaaaggcgta gcaaaacggc
    24721 agagcaagta atgctctgca tggtgcagtg attaatttca ttgtgttaat ccatgagcta
    24781 aagaaaaagt acagcctttt atcgttgtaa ttataaaaat ataaaggcag aaactatttt
    24841 ccctctcctc atccaggaag agggaaaatg cctgctcatt ttttattatt ctgcttaatc
    24901 aacattgctg gctggcgtcg cttcaatatg gcgctcttca gccgttaatc catttatata
    24961 tttactgtat ttccaccacg caaagccaag gaagataaca ataccgccga cgttataaaa
    25021 aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa gcgacaaacc ctacggcaaa
    25081 tatcgcaatc agcattgaaa caacgataat accggtgcgg cgggagccca tacggaaatc
    25141 gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa gccagcatga tgaataacgg
    25201 cggaagcatg gacgctgcgg cggtcatatt aataatagta ttcatcagat cctgcacggt
    25261 attggaaccg agcatcggga taatcatcag cgggatgacg atcaggaact ggatccacgc
    25321 tgcgcgcgcc ggaacgccgt tttcgttcag ttcgacggtt ttcttaccaa agatgccttc
    25381 cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc cacatcagca gggaaccgaa
    25441 catcgcggta aaggacacca gaccgacaaa gcgattcatc aacgcttccg gtagaccaaa
    25501 atacgccgcc atgccgtgga atacctgcac cgatccgccg gtaaatttca actctttgct
    25561 gctgacgaag acgttaatca gcaccgagga gacggaatac agtacgccga taaaaatccc
    25621 ggcgaggatg atcactttaa cgaacgattt cgaaccgcct ttgacgtcgt taacgtacac
    25681 agcgacggac tccgcgccac ctgcggccat aaagatccag gtggtaacgc cgaggaacgc
    25741 ccagttgaag ttcgggatca tcgcgtcaac ggtgatggcg tcagcaggct gtacgccgcc
    25801 aaccagcgcc gtacctgcca gtaaaatgta ggagagcgtt aacagcagca tcagcgttga
    25861 agtgacggag gtaattggcc ccaacatttt cgccccgttg gtggaaaccc aggtggagaa
    25921 ggcgaacagc accatactga taatggtggt ggcaaccggc gtcataatat attcgtatcc
    25981 gaggaaggca tacgaagcat aagcaataac gcgcggcaac agtgaggtga aaaagaacag
    26041 gtttacgaac cagtaggtat aggcagtaat aaatgcccaa cgtccgccca gcgaactttt
    26101 tacccacgcg tagacaccgg cttctgagtt tttatttaac gaaacaaatt ctgcgatgat
    26161 cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa aacatcggtg ccgaggccag
    26221 tccaagctca atattattat tgataacgtt gttaaagctg taaacggcgg caaaagtcag
    26281 cgagagcaag ccgaatttgc cgattgtatt acgtttggta tcagacataa cacatctccg
    26341 aaaatgagta gggtagcggt ggcggcaaac tccttttgcc gccgtagttg ttatttgtta
    26401 tggaaataac catcttcgat ggtgactttg agaaccactt ttttgaccgc gttattgcag
    26461 ataaaccgat acgcttcatg gatatcgcaa ataacgattt gcccttcgtg gacctcaacg
    26521 gtttctccgc agccttttaa atattcacgg tcagtttcat cacgataata ttccactacc
    26581 tgtaatgtct ctttcggcgc atattcaatt ttttgctgcc cttgcaggta gtaatgcact
    26641 tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg cggagtctgt ctccacgcgg
    26701 taagtcaatg agtcaccgat ggagtgggcg acgccaggct gaatgttgtc gatattttca
    26761 atcgcttcaa cgcagcgttg ccacttcttg ccagaggcgt aaatctggcg gaactgttct
    26821 aagttatcga tgatcctcat tgcttattct cgctgtgcaa attcgtggaa aagaaccctg
    26881 cgccgaactc atacgacgcc aggctttgcg cggtagcttc tccgccagaa accggcagca
    26941 acgtaaagcc gtagctgaag tcacggaacc agacgcgcca ggagtccagc acctcgctgc
    27001 cccaggagtt ggagccgagg ccaagcagct ggtgatcgag gttcagggtg atgtcatcac
    27061 tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt ttcctgggta tagtgccagg
    27121 cgctgaagtt aattgggcgc tgcggaacca ccagcagacc gttaccgtgg cggttagtca
    27181 gtgccgtcca gcggacatgc tgacggttac cgttgttctg cgggaaggga tagttctcga
    27241 acatggcatc gacggtgctg cgccagatat cgatgatgtt agcctgctgg ctgtcggcgt
    27301 agttttcgcc cggtccacga ccgtaatacg ccacctgatc gtattcgccg ttaattccca
    27361 tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc gtaacgctcg ccggaaagcg
    27421 ccacgttaac ctggccatcg gcagcgatgc gccagatgta ggtgcagcgc atcccgaagt
    27481 caaacaccgg cggggcaata actgtgcggc tgatgatcag cacttcacca tcgctctgtt
    27541 ctacggcaaa gtcgcgcaga tgttcctgca tgatctgcaa atgattcggt tgccacagcc
    27601 cttcatactc ctgcttgtgg ttgtcgatca tcggcttgaa gaagttgatc tttggctcgc
    27661 gagtcagcag cgattcgcca ttcacctgcc aggatgtcgg tttgccactc atttttgaga
    27721 aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa acgatcgtct tccagcgtca
    27781 gcggacgcgc attatttggt gcgaaaggca ctggctgcgc ggtgttttcc ttcagcggga
    27841 actgataagt ggcgataggg tgtccggctt cgctgtagcg ggtgcgggaa tctttggtca
    27901 ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg cagcgtgatc tgcaaggggg
    27961 cttcgctgtt cggcgcaacg tcgcgcagtt taatctgctg cgtcgcgagc gtttcacctt
    28021 cggcgcgcac ctctgcgtgc agggtgtagt catcaagcgt ggtaaaccac agtttatttt
    28081 cgactttcaa ctcgccgcga gtcagatccc gcgcgtggat ttttaccggc gcgataacct
    28141 gtttgtactc tttcaggccc ggtcccggcg tctgatcgga atagatcaaa ccatcaagac
    28201 agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac cagacattgc
    28261 cgtggtcgtc ctgtgcctgg atcccgtggt cgcaccactc ccagacataa tgaccctgaa
    28321 tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcccggt ccgttcccca
    28381 tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca ccaaactcat
    28441 tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca gcatcgcgat
    28501 cttcttcgta atgcaccagt cgcgtgtcat ccagcgcttt cgccgcatgg tacatcgcgc
    28561 ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc gacggatggt
    28621 ttttctgcgc gtggatatgg cgaacaatgc gctcgacgta gactttttcc cactgcggat
    28681 cgtcggtaat acggctaata tcgccgacat tagcaaagcc gtgcgattcg acgtcggttt
    28741 ccgccatcac aaacaggccg tagatatcac acagttcgta aaaacgcgga tcgttcgggt
    28801 agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga tctttctcga
    28861 cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg ttgacgccgt
    28921 gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg atatcacgga
    28981 agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc agcgtcatga
    29041 ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg acagtaaaag
    29101 caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg ctgtgcacca
    29161 cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag gcggcgagat
    29221 tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca aagtcggtac
    29281 gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca tcgcggaaga
    29341 tccccgctga ccaccacata tcctggtctt ccacgtaggt agagtccgcc cactgcatca
    29401 cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca aactctgcgg
    29461 tcaggcgact acccttgctg aaacccacat actgaccgtt aacatagact tcaaaatagg
    29521 tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg ctgagggtga
    29581 aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca tcgatgggga
    29641 acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc cacatggcgg
    29701 ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct ggtacttgca
    29761 gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac aggctgctgg
    29821 tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag tacgcacgcg
    29881 gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata gggcctcccg
    29941 gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct ggggagttta
    30001 gtaaaatttt tactaaaaag tagcaggtaa atgcatttac tttaactgga tcacgaaaat
    30061 aaacggcagc actgtccggc aaaaacgatt gccggacagt gtgtaagggg atttaacggg
    30121 tcgtgccgcg cagttttaat ttgctgggaa cgaagactaa cagcggcagc gcgcgaccat
    30181 cgcgggcttt ttcatacacc aggttaacgc cctgacttcc catcatctcg gaatggatgc
    30241 gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg gatatcgtta acgctgataa
    30301 gcgaaatatc ctgtgggatg ttcaggcctc gttcatgaat tgcccgcagt acgccgatag
    30361 caatggaatc ggaagcaaca aacagtgcct tcggatagtc ttcccgcgcc agcatttgtt
    30421 ttgccagttc ataacccgac gaactggaaa aaccgccgcg ccagatatcc tcttcgcgta
    30481 ccacttgttt cagtcggcca tattccgcaa aggcgacctc acgaatatcc gccttgccag
    30541 gctcatcttc accgccaata aaaccaatac gattaacgcc ctggttgata tagaagtcga
    30601 tgatttcttt actgatgcgt gccagatcga tatccaccgc atcgtaaccg ctgccgggtt
    30661 cgtgaaagtc gataaaacag atattgtcgg tcaacgcgct ggcagcggcg cgcagggcgg
    30721 gcgtgggttt gccgacaatt aaaataccgg tgacgttttt aatgtctggt aagccgctgt
    30781 gttcataaca gttggtgagc tcgatgccca gcttttcgca ctgggtttca atgccgtggc
    30841 ggatcgccag atagtaagga tcgttgatct ccagctcctg ctggtagctg tagatagcca
    30901 gaatatggtg ttggttgact gcacctgtct ggagtttacg ggcactactg gtcttgtact
    30961 ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc ttctttcaca ttcaatgtcg
    31021 gatcgtcatt taagaccctg gatactgtcg ccagggatac gccagcttcg attgcgatgt
    31081 cttttagtgt tgccatgttt acctttttta ctttccctga tgtgcgcccc cattgtagag
    31141 gaagtgctta tcgggtcacc tgttttcagt aaataaagcg cgtttctttg acgctgtaac
    31201 ggtaacacag cggctcacga atgtgatcac gcctgcattc tccattttta gcctacagga
    31261 ttgagaaaaa cttttgctat agtttagtaa aattttaccc taagggagga agcaatggaa
    31321 accgtggctt acgctgattt tgcacgtctg gaaatgcgcg tcggaaagat tgtggaagtg
    31381 aaacgccatg aaaacgccga caagctgtac atcgtacagg ttgatgtggg gcaaaaaacg
    31441 ctgcaaaccg tgaccagcct ggtgccgtac tacagcgaag aagagctgat ggggaaaacg
    31501 gtggtggtct tatgcaatct gcaaaaagcg aaaatgcgtg gtgaaacgtc ggaatgcatg
    31561 ctgttgtgtg cggaaaccga tgacggcagt gaaagcgtgc tgttaacgcc ggagcggatg
    31621 atgcctgcgg gcgtacgcgt cgtataaaaa gatcggatgg cgacgtcgta tcgccatccg
    31681 atttgatatt acgcttcttc gacacttact cgcatggccg ccagcgcctt acgcgccgct
    31741 ttgatcacca gttcacactg ttcaatggtc agtgtcagtg gcggttcaat gcggatcgtt
    31801 ttggcgttat tgagcgttcc ggccaccagt acgcgctggc ggaacatctc gctggcaaag
    31861 ttatagccga tttcgttatc aacaaactca atcgccatca acatcccttt accacgcgct
    31921 tcctgtacca gatcgggata ttcccgcgcc agttgacgga aaccgtccag caacatatcg
    31981 cctttttgct cagcctgagc cggtaagttc tgctccagca acacattgat ggtcgccagc
    32041 gccgccgcac aggccagcgg gttgccgcca aaggtggtgg tatgcaggaa tgggttgtcg
    32101 aacagaactg aaaacacctc ttcagtggcg atggtcgcgc caatcggcat cacgccgccg
    32161 ccgagcgctt tggcaaggca gaggatatcc ggctgtacgt tctcatgctc gcaggcgaac
    32221 atcttgcccg tgcgccccat gcccgtttgt acttcatcga ggatcatcag tgcgccgaac
    32281 tcatcgcata gcttacgtac ggcggtgaga tagcccggcg gcggcagaat tacgccacct
    32341 tcaccctgaa tcggttcgag gatcaccgca gccacatcat caccggtttt tttgcactcg
    32401 ttaagagccg tgcgcatggc ttcgatattg ccaaacggca catgacggaa ccctggcagc
    32461 agtggcataa acggtttgcg gaaggtcgat ttcgccgttg ccgacagcgc gccaagtgat
    32521 ttaccgtgga acgcgccgct ggtggcaata aaagtaaatt tgccgcgcgg cgactgataa
    32581 gccttcgcca gcttcagcgc cgcttcaacg gactcggtgc cgctattaca gaagaagctg
    32641 tatttcagtt taccgggcgt tagcgcagca agggttttcg ccagcatcgc ccgtaacgga
    32701 tcgagcagct cctggctgtg cagcggttgt ttcgcaagtt gattctgtac ggcggaaacc
    32761 acaactggat tacggtgccc cacgttgaaa attccaaaac ctcccaggca gtcgataaac
    32821 tcctctccct gggtgtcgac aagcgtattt aaacctcccg cttgccactc tacggctccg
    32881 taatccccgc cggcggtaac agatttgcga tactctaaaa accccggatt gacatgctct
    32941 ttgaagtatt caatcacctc tcggttaagt gctttcatct cctcatgatc cagcgttcgc
    33001 ttttcaatga gattcagggc gtgggcgctg cacgctaaag ccgatgcgct cgaaggtaac
    33061 ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat accgatttaa gtattgcagg
    33121 gattgcgcca ctccggctgc gatcgcgaaa atcgggataa acacaaatgc ggttatcatt
    33181 tgcgcaatat ttaatgctat atgttaacat ttgttgttat tttgcacggt ttttgtcggg
    33241 tatggatttg atgattgctg cgcaaatgca cgctttcagg gcattaactg cctgattttt
    33301 gggcaagata attaacgttt tcgtatagta acagagtgaa agtattttaa atcaacgaat
    33361 tagatttgtg ctttaatcac aaattgcgat ctaaatcaaa ttaatcggtt aaagataacc
    33421 gcagcggggc cgacataaac tctgacaaga agttaacaac catataacct gcacaggacg
    33481 cgaacatgtc ttctcatccg tatgtcaccc agcaaaatac cccgctggca gacgatacca
    33541 ctctgatgtc cactaccgat ctgcaaagct atatcactca tgctaatgac acttttgtgc
    33601 aggtgagcgg ctttaccttg caagagttac aagggcagcc gcacaacatg gtgcgtcacc
    33661 cggatatgcc aaaagcggcg tttgcggata tgtggttcac cctgaaaaaa ggggagccct
    33721 ggagcggcat cgtgaaaaat cgccgcaaaa atggcgacca ttattgggtg cgggccaatg
    33781 cggtaccgat ggtgcgcgag ggaaaaatca gtggctatat gtcgattcgt acccgggcga
    33841 cggatgaaga gatcgcggcg gtggagccgc tgtacaaagc gttgaactcc ggacgtacca
    33901 gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg gctgggtaaa ctgccttcat
    33961 taccgcttcg ctggcgggcg cgtggcgtga tgaccctgat gtttatcttg ctggcggcca
    34021 tgctttggtt tgttgctgcc ccggtggtga cgtatttcct ctgtgtgtta gtggtattgt
    34081 tggcaagcgc ttgttttgaa tggcagattg tgcgcccgat agaaaatgtc gcccgtcagg
    34141 cactgaaggt ggcgaccgga gagcgtaata gtgttgagca cctgaatcgc agcgatgagc
    34201 tggggctgac attacgcgcg gtagggcagc ttggcctgat gtgccgttgg ctaattaacg
    34261 atgtctcaag ccaggtgtcc agtgtcagaa atggcagtga gacgctggcg aaaggcaccg
    34321 atgaactgaa cgaacatacc cagcagacag ttgataacgt tcagcaaacg gtggcgacca
    34381 tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac ggcgtcggct gccgataaac
    34441 tttctatcac cgccagtaat gcggcagtgc agggtgggga ggcgatgacc acggtgatca
    34501 agacaatgga cgatatcgcc gacagtaccc agcgcattgg caccattact tcgctgatta
    34561 acgatattgc gtttcagacc aatattctgg ccctgaatgc ggcggtggaa gcggcgcgtg
    34621 ccggcgaaca gggcaaaggt tttgcagtgg tggcagggga agtgcgtcat ttagccagcc
    34681 gcagcgctaa tgctgccaac gatattcgca agctgattga tgccagtgct gataaggtgc
    34741 aatccggttc gcagcaggta cacgccgccg gacggacgat ggaagatatt gtggcacagg
    34801 tgaaaaacgt cacccagttg atcgcccaga ttagccattc aacgctggaa caggccgatg
    34861 ggctttccag cctgacccgt gcagtggatg agcttaacct gatcacccag aaaaatgccg
    34921 agctggtgga agagagtgcg caggtgtcgg cgatggtgaa acaccgcgcc agccgactgg
    34981 aagacgcggt gacggtgctg cattaatcgg tatgccggat ccggcggttg gagcacaatg
    35041 cctgatgcga tgctggcgca tcttatcagg cctgcggggt gtgtagcggg tgtaggccgg
    35101 ataaggcgcg tcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgat
    35161 taatccagtt gcgtaatatc aaaagcggcg cggtcgatca ccgcaatgat cttttttatt
    35221 tgtgcatcac tgatatcgct ctggttgacg cgtaaatcca gcaccgcttt aaaattatcc
    35281 agcgcccgct tcatttgcgg gttctggcgc agcgccgcgc caacgcaacg cgctttgatg
    35341 cgttcttcaa tcatctccac ctgttcgcgg ttttcttcca gccactgcgc tccttgttcg
    35401 gtcagcgcaa tctgcttctt acctccttcc tcttcgcgga tggtaatcag cgactgctcc
    35461 tgcagaaaat ccagcgttgg gtagatgacg cccgggcttg gggtgtaatt cccctgggtt
    35521 agattctcaa tcgctttaat caattcgtaa ccgtggctgt catcgcgcga gagaatatcc
    35581 agaatcacca gacgtaattc accgtgacca aagaaacgtt gccgacgacc gccgccgcga
    35641 ccgtggcgac caccgcagca ttgaccgtgt tcatgctggt gaccgtgtcc gcagtgctcg
    35701 tgttctgact tctcaccttt gcagcagccc tcatggcgtg gctggccttc atgtttacaa
    35761 cacccttcgt gatgatggct catatcagcc tccttttgct taactagata tatctaattt
    35821 atatctgata tgagcgttct tgcaagtgta aaaatgattt ttaaacgtgg actcatctca
    35881 cacgtaggcc ggataaggcg ttcacgccgc atccggcgtt gagtaatgat gcctgagaag
    35941 aggcggttcg tcttatcaga ctatagacat atcagttcta caaatcgctt gcatttatca
    36001 tgattaacaa tcattatcat ttgcgagttt tatttagata tatctgatta ccatcacgaa
    36061 ggcgataaca atgaataaca ccccccgcta cccgcagcgc gttcgcaatg atctgcgctt
    36121 ccgtgaactg actgtgttac gcgttgagcg catcagcgcc ggttttcagc gcattgtcct
    36181 cggcggcgag gcgctggacg gttttacatc gcgtggcttt gacgatcaca gcaaactctt
    36241 ctttcctcaa cctgacgctc actttgtgcc gccaacggta acggaagagg gcatcgtctg
    36301 gccggaagga ccacgcccac cgtcgcgtga ctatacgccg ctgtatgacg aactacgcca
    36361 tgaactggcg attgatttct ttattcacga cggtggggtc gccagcggct gggcgatgca
    36421 ggcgcaaccg ggcgataaac ttacggtggc aggtccgcgc ggttcgctgg tggtgccgga
    36481 agattacgcg tatcagctgt atgtctgcga tgaatccgga atgcccgcat tgcgccgccg
    36541 cctggaaacg ttgagcaaac ttgccgttaa accgcaagtt agtgcgctgg ttagcgtgcg
    36601 ggataacgcc tgtcaggatt atctcgcgca ccttgatggt tttaatatcg aatggctggc
    36661 acatgatgag caggcggtag atgcgcgtct ggcgcagatg caaatccctg ccgatgatta
    36721 cttcatctgg ataaccggcg aaggtaaagt cgttaagaat ttaagccgcc gctttgaagc
    36781 ggaacagtat gacccacagc gggtacgtgc agcggcttac tggcacgcaa agtaactgat
    36841 atataaaaag aaaaaggctg acgatttctc gtcagccttt gctatatctg gtggcccctg
    36901 ctggacttga accagcgacc aagcgattat gagtcgcctg ctctaaccac tgagctaagg
    36961 ggccatggta gcggattata aagtaactcc gtgtcgcaat ccagccatta ccgcgcgcct
    37021 gctgttttta taaacaatgt attttcaatc cgttatactt ttcctggtga tgttagaaag
    37081 gagtaaatat ggttgaggat attttggctc cagggttacg ggtcgtgttt tgcggtatca
    37141 accctgggct ttcatccgcc gggactggtt ttccctttgc tcatccggca aatcgcttct
    37201 ggaaggtgat atatcaggcc gggtttaccg accgtcagtt gaagccgcag gaggcacagc
    37261 atctgctgga ttatcgttgt ggcgtcacca aactggtaga ccgtccaacg gtgcaagcca
    37321 atgaagtttc aaagcaggag ctacacgcag gcgggcgtaa gctgattgaa aaaattgaag
    37381 attatcagcc gcaggcgttg gcgattctgg gcaaacaagc atatgaacag ggattcagcc
    37441 agcgcggtgc acagtggggg aaacaaacgc tcaccattgg ttcgacgcag atttgggtgc
    37501 tgccaaatcc cagcggttta agtcgcgttt cactggagaa actggttgaa gcgtatcgcg
    37561 agctggacca ggcgctggta gtgcgtgggc gataaaaaac gccaccgaaa aggtggcgtt
    37621 tgtgcagagg gcctagtgcc gggtgcggcg taacgcctga tccggcctac cgattaatcg
    37681 tccaggaagc tacgcagcac ttcagaacgg ctcgggtgac gcagtttgcg cagcgccttc
    37741 gcttcgatct gacggatacg ttcgcgggta acgtcgaact gtttacccac ttcttccagc
    37801 gtgtggtcgg tgttcatatc gataccgaaa cgcatacgca gaacttttgc ttcacgcgcg
    37861 gtcaggccag ccagcacgtc gtgcgttgcc gcacgcaggc tttcggtggt cgcagaatcc
    37921 agcggcagct cgagggtggt atcctcgatg aaatccccca gatgcgaatc ttcatcatca
    37981 ccgatcggcg tttccatgga gattggctct ttggcgatct tcagcacttt gcggatcttg
    38041 tcttccggca tcagcatacg ttcagccagt tcttccggcg tcggttcacg gcccatctct
    38101 tgcagcatct ggcgagaaat acggttgagc ttgttgatgg tctcaatcat atgcaccgga
    38161 atacggatgg tgcgcgcctg atccgcgata gagcgggtga tcgcctgacg gatccaccag
    38221 gttgcgtagg tggagaactt gtaaccacgg cggtattcga atttatcaac cgctttcatt
    38281 agaccgatgt tgccttcctg aatcaggtca aggaactgca agccacggtt ggtgtatttc
    38341 ttagcgatag aaataaccag acgtaagttc gcttcaacca tctctttctt cgcacggcgg
    38401 gctttcgctt caccgatgga catacgacgg ttgatatctt taacctgctc gatggtcagg
    38461 ccggtttctt cttcaatctg ctgcagtttt tgcagggcgc gatgcacttc ttcagagaca
    38521 tcgtgcagtt tttccgacca cggcttgttc atcgcaattg ccgcgttgaa ccaggtatcg
    38581 ctggtttcgt tgccggtaaa cagggtaatg aagtttttct tcggcatttt gcactgctca
    38641 acgcagagct tcatgatcag acgttcttgc gtacgaacgc ggtccatcat gacgcgcatg
    38701 ctgttgacca ggtagtcaaa ctgcttcggc accaggcgga actgtttgaa tacttcagac
    38761 agtttcagga tctcttcctg agcggtagcg tgactgcgac ctttcgcttt gatggtgtca
    38821 cgcgttacaa cgtactgagc gcgtagttcc gcaaattttt cgcgagccag ttccgggtcg
    38881 atgctgttgt catcatcggc gctgtcgtca tcgccatctt cttcgtcttc atcttcgtca
    38941 tcgtccagat cttcctggga aagctcagaa ccgacgtgag tggcggtagg tgccagatct
    39001 tcttctgcgt tcgggtcaac aaagccggtg atcagatcgg acagacgcgc ttcttctgct
    39061 tcaacacgat cgtactgttc cagcagatag gtgatcgctt ccggatattc agcaacggag
    39121 cattgaacct ggttgatccc gtcttcaata cgcttagcga tgtcaatttc gccttcgcgg
    39181 gtcaacagtt caacggtgcc catttcacgc atgtacatgc gtaccgggtc agtcgtgcgc
    39241 ccgatttcag attccacgct ggaaagcacc tgcgcggcgg cttcggcagc atcttcgtcc
    39301 gcggtgtttt cagccagcat cagatcatcg gcatccggtg cttcttccat cacctgaatg
    39361 cccatgtcgt tgatcatttg gatgatgtct tcgatctgat ctgaatcgac gatatcttcc
    39421 ggcagatggt cattgacctc ggcataggtc agatagcctt gctccttacc acgggtgaca
    39481 agaagtttca gctgtgactg cgggttttgc tccataagac ggtatccaca cttatttcat
    39541 gaggttggtg ttgggcgatt gacccgatgt ccttcagccg ttaacgctga aatccaacgg
    39601 ttgtttaccg ccaacgataa ttacgagggc gtacttatat atttgccgct gcctctcagt
    39661 gcggctgtcg ggggcttccc gatcgctctt cggcacttaa gccgttaaat cactttttcg
    39721 ccagctcctg gtttaatgtc cagagctcca ggcgttcttc gttgcttaaa ccatgcgtgc
    39781 gctcacgagc gattaactct tcctggcgca gttcaagcag cgaatcaaac atatggttga
    39841 gtgagtcggt gaaggtttgc tcagcaatat tcttatctgc tatatcgtcc cacatcgaca
    39901 gtttttcaag ggtggcagca ttatttgtac cacgatagtg ctctaaaagt tgcccggtgg
    39961 tcagacctgg ctgggagaga caagtgttga ccagttctct gaataagcca agtccaggga
    40021 gcttattttc atccagattc tcaagcggcg ggaccaacgt cgctaattct ggattttgca
    40081 ccagcaaccc tataagtata cgcatggtcg tgcgttttag ctgcggaaca gggcgagaaa
    40141 cgccgctctc tgccgctttt ggcattaatc gttcaagctg gctgtcatca agtatgccta
    40201 atttgttgcc taattcctga cgaagatata ttcgcagcgt ttcgcccggc acttgcgata
    40261 tcaatggtag tgccagcgta ctcaaacgtg cgcgcccgtc aggggtactc agatcaactt
    40321 gcggcatcag actgttaaac agaaatgcgg agagtggcat cgcctgctcc atccgcgctt
    40381 caaacgcttc tttaccttct tttcgtacca gcgtgtcagg gtcttcgcca tcaggcaaaa
    40441 acataaagcg tagctgacgg ccgtctgtca tgtaaggcag cgccgtttcc agcgctcgcc
    40501 aggcggcatc gcggcctgca cggtcgccgt cataacagca aatgacattg ttggtcgcgc
    40561 ggaacaacag ttgtatgtga tcggcggtgg ttgacgtacc taacgacgca acggcgtaat
    40621 taatgccgta ttgcgccagc gccaccacgt ccatatagcc ttcgaccaca agcagacgat
    40681 tgggttcagc gttatcctgc tgcgcttcat aaagaccgta aagctggcgg cctttatgga
    40741 aaatgtctgt ttccggcgag ttcaggtatt tgggggtatc gttgcccagc acgcgcccgc
    40801 caaaaccaat cacccgaccg cgtttatcgc gaatggggaa catcacccgc tcgcggaaac
    40861 gatcgtaact gcgtccctga tcgttagtga ccaacatccc cgcatcaatc aatgactggc
    40921 gattttctgg attgccgcca aaccgcttca ggacgttgtc ccagccgggg ggcgcaaaac
    40981 caatcgcaaa gcgagcgata acctcgtggc ttaatccgcg tttttccaga tactggcgcg
    41041 cagacgtggc aacaggttgt tgtaaagatt gttggtaaaa cgtattcaga ccgtccatca
    41101 actgataaag cgtttgcctc tgatggcgct cgatctggct ggggccgctg cctgcttcaa
    41161 atggcacttc aagattgtgc attgctgcca gctcttcgac cgtttcgacg aactcgagct
    41221 tgtcgtagtt catcaggaag tcgatcgcgt tgccgtgcgc gccacatcca aagcagtggt
    41281 aaaactgttt ctcaccgtta acggtgaagg acggggtttt ctcgttgtgg aatggacaac
    41341 acgcgtggaa attcttgccc tgctttttca gcttcacacg ggcatcgatc agatcgacga
    41401 tgtcagtgcg tgccagcaga tcattaatga atacgcgtgg gattcgtcca gccataggcc
    41461 ccgattttta gcaattcata aacgaaaata agccgcgcat tcctttcgga agcacggcct
    41521 tacaactaca actcggtctg atcggagagc aacgctctcg gggaattagt acagacgagt
    41581 gcggcgtgcg ttttcgcgag ccagtttctt cgcgtgacgt ttcactgcag aagctttagc
    41641 gcgcttacgt tcggtagtcg gtttttcata gaactcacga cgacgaactt ccgccagaac
    41701 acctgctttt tcgcaggaac gcttgaagcg acgcagagct acgtcgaacg gctcgttttc
    41761 acgtacttta attaccggca tgtgcctctc acctttgatt aattcggttt gccgctggca
    41821 tcaacgccag cttatttcaa aatggtgcgg aattttactg caattgctgc tgctttgtaa
    41881 agcaccgcgg ccttttttga aagggacttt tataagggtg aggagtatac acgaggcttt
    41941 ctccaggggc gaacaaagtt ttacatcaac ccgcattggt cctacactgc gcggtaataa
    42001 agcgaggtaa aacaagtcat gcgtgtactg ggtattgaaa cttcctgcga tgaaaccggc
    42061 atcgccattt acgacgatga aaaaggtttg ttagccaacc aattgtatag tcaggtgaaa
    42121 ttgcacgctg actacggcgg cgtcgtgcct gaactggcct cccgcgatca tgtgcgtaaa
    42181 accgtaccgt tgatccaggc ggcgctaaag gagtctggtt taacggcaaa agacattgat
    42241 gctgtggcct ataccgcagg ccctggatta gtcggcgcgc tactggttgg cgcgaccgtg
    42301 gggcgttctc tggcgtttgc ctgggacgtt ccggcgatcc ctgtacacca tatggaaggg
    42361 catctgttag cgccgatgct ggaagataac ccgccggaat ttccgtttgt tgcgctgctt
    42421 gtttccggcg gtcatacgca gttaatcagc gtgactggca ttggtcagta cgagctgctc
    42481 ggcgagtcta tcgatgatgc cgccggggaa gcgtttgata aaaccgcgaa gctgctgggg
    42541 ctggattatc ctggcgggcc gttactgtcg aaaatggcgg ctcagggtac tgccgggcgc
    42601 tttgtcttcc cgcgtccgat gaccgaccgt ccggggctgg atttcagctt ctccggcctg
    42661 aaaaccttcg cggcaaatac cattcgtgac aacggcaccg acgaccagac gcgtgctgat
    42721 atcgcccgcg cctttgaaga tgcggtggtc gatacgctga tgattaagtg caagcgggcg
    42781 ctggatcaga cgggctttaa gcgactggtc atggcgggcg gcgtgagtgc taaccgtacg
    42841 ttacgggcga agctggctga aatgatgaaa aaacgccgcg gcgaagtgtt ctacgcgcgt
    42901 ccggaatttt gtactgataa cggcgcgatg atcgcctatg ccggaatggt gcggtttaaa
    42961 gcaggcgcga cggcggatct cggcgttagc gtgcgtccgc gctggccgct ggcggagtta
    43021 ccggctgcgt aaaactattg ggtgccggag agcaatttcc ggcaccgtcc tcacttaaag
    43081 caacaccacg ggcatccaca gtaagccggt aatcaccagc aatacgagga atatcagccc
    43141 aaaaatcgcc cccagtcgcc agtaatccgc cgttggcaga taaccactgc cgtagtaaat
    43201 cggactgggg ccggtggcgt atggtgtaag aatgctcccc agaccaattg ccgcaccaac
    43261 catcaggcag aacaccggca gcgggatttc cggcatcgcc agcgcagcgg caatcatcat
    43321 cggtgcgaga gcggaggtat acgccgtggc gctggcgaaa aagtagcgca gtagataaaa
    43381 caccgcaatc aacgccacca tcaccatcgt tggcgaataa ccgcttaagc tgcctgctaa
    43441 cagtttgcca aaccagctaa taaaaccggt gttgttgagt ccggtggcga gggtgataag
    43501 cgaggccagc cagaagaaaa cgttccacgc cgctttatta ctgacaatgt cgtcccaact
    43561 gataatgcgc agaagcagca tcagtgccac cacgctgtaa ccgaccatcg cggcatcgat
    43621 ataatcaccg ccgaaaatcc acagcaccag cgcgcctacc atcagcccca gcatccgttt
    43681 ttcacgcgaa cagagcgggc ccattgcctg cagttccgtc tctgcccagc gcggcacctg
    43741 atcgccagac ttcagtaccg gcgggtacag cacgtaagcc agccagggaa ccagcagaac
    43801 cagtaaaatg ctgagcggca acatcccgag gaaccagtcg ccccaactca gcgtggcgtg
    43861 agatgcgctt ttcatcagtc caattaacag caagtttggt gccatcgccg tcaagaaaat
    43921 ggcgctggtc acgcagtcgg caacaatccc catccacatg atgtacgagc caatgctgcg
    43981 cgaactgctg tcgtttggtt gtgattgata gagcggtggc aggttacgga tgatgggata
    44041 gataatcccc gcaccacgcg ccgagttgga cggtgttaca ggtgcgagga tcagctcgga
    44101 gaacatcacc gcatagccga gaaacagcgt gcgatgtccc atctttttca ccagaatcag
    44161 cgcaatgcgg cgtccaagcc cggttttttc atagcctgtg ccaaacataa aggcggcgaa
    44221 aatcagccag ataaccgaat tagaaaaacc ggaaacggcc cacgagaggg attttgcagt
    44281 gaatttaaag cctggctgag cgagctgctc cgggctgaac agcagccagg gagagagtat
    44341 ggcgatgatg gatataccca ccatcgccac cacggcaccc ggcacgggtt cgaggatcag
    44401 tccaacgatc acgccagtaa aaacggcaaa gtagagccag gtatggttct ccagccctgc
    44461 gggaaccgga agtagagcaa taatggcgat gaccgccagc ggcgcaagat atcgccacca
    44521 ttcagtggaa ggtttcatcg tgttatgcct gcgaaaaagg agaggggtga gcctctccgg
    44581 gagggttatt tgatgtaatg gacgtgctcg cagatctctt ccacgatggg atcgcggcgt
    44641 tcggcgaaca gctttttgtt ttcggctatc aggttgttgc cgtgggtatc aatagagaca
    44701 atcagcgggc cgaactcttt gacccggcag acccacagtg actccggcat tccgagctct
    44761 gtccagtgca cttcttcaat ctcttccacc tgggttgccg ccagcaccgc gcagcctgcc
    44821 gggaaaatca catgtagcgc cttgaatttc tggcagcctt cttcggtcag cggccccata
    44881 ccacctttgc caaccaccag tttcacgccg gtctgctcaa taaattcacg ttcaaaactt
    44941 tccatacgca tactggttgt cgggccgacg gagaccatct cccatttgtc gccgtttttg
    45001 cgcacgatgg ggccagcgtg gaaaatcgct ttgccgttga gatcgtaagg gatcggacgt
    45061 ttcagttcga tcaaacggcg gtgacaaacg tcgcggcagg tcaccagcgt accggtcagg
    45121 tagatcacat cgccgacgcg aatatcttgc agatcttcag ctttgatcgg ggttgtcagg
    45181 atctttttca taacgcgctc cgggtgtgag acagattttc aaaggtgaga tcggcatgaa
    45241 ccagcagcgt gccgcgacga tgcgcccagc agccggtaga gacagcaacg ccgatggttg
    45301 acggatggcg ggcggcagat tcgatatgta cgcccatcac tgaactgttg ccggtcagcc
    45361 cttgtggacc aatccccaga cggttgagtc cttcttccag gcgtagctcc agttctgccg
    45421 cttttggatt gggatggcgg ctgccaatcg ggcgcaaaat ggctttacgc gagagtacgg
    45481 cggcggtttc caccgaggtg gcgatgccca cgcccaccag taccggtgga caggcgttta
    45541 cggcgagggt ggagatattt tcgaagacga atttcaccac gccttcgtag ccttctgacg
    45601 gcattaacac tttcgagcgg ccaggtagcg tgcagccgcc gcctgccatg taaacttcga
    45661 tttccgcatc gtcattgtcg gggatgatgt cccaggtgac ccacggtacg ccgctaccgg
    45721 tatttttgcc ggtgtttact tcgtcaaaaa tttctaccgc attgtgacgt agtggcgctt
    45781 tgacggttgc ctcttccacg gcttgtttga gtatgctttg cagctcgcca agcagtggga
    45841 agcgggaacc gactttaacg aagaacataa tctccccggt gtcctgacag gcaggacgat
    45901 tcaggtcaat cgctttttgc atgttgtcga acatcgtatg gtagataatt ttccccatcg
    45961 acgacgtttc ggcatccttt agctgtttta gtttatccac cacgtcatca ggcattcggg
    46021 tagaaatcat ggcggtaaag ttagcgacaa tctctgtcaa cttattcact gcctgttgct
    46081 tattactttc gctcatcatg gcaactccag tttttgtatt tgtctggaaa aattcaccgc
    46141 cacaaactac cactaaaaat gtggttttat attttttgcc accgtgaata tgtaattaac
    46201 agttcgtgaa tcaacgcgaa aataagaatg aatattgcgc cagagcaggg aatggcaatg
    46261 ttttgcgaaa tacactgcgc ttatcacaac tgcggataat taacgatgct gaatagctgg
    46321 cctttagcca aagacttgca ggtactggta gaaatcgttc acagcgggag ttttagcgcc
    46381 gcagcggcga cgcttgggca gacgcctgca tttgtcacca agcggatcca aatccttgaa
    46441 aatacgctgg caacaacgct gctgaaccgc tccgctcgcg gcgtggcgct gactgaaagt
    46501 ggccaacgtt gttacgaaca tgcgttggaa atcctcaccc aatatcagcg actggtcgat
    46561 gacgtcacgc aaatcaaaac gcgcccggag gggatgattc gtattggttg cagttttggt
    46621 tttgggcgca gccatattgc gccagctatt accgaactga tgcgcaatta tcctgagtta
    46681 caggtgcatt ttgaattgtt cgatcggcaa attgatttag tgcaggataa tattgatctg
    46741 gatattcgta ttaatgacga aattcctgat tattatattg cgcatctttt gacaaaaaat
    46801 aaaagaatat tatgcgcagc acctgagtat ctgcaaaaat atccgcagcc acaatcccta
    46861 caggaattaa gtcgtcatga ctgtctggtg accaaagagc gcgatatgac ccatggaata
    46921 tgggagttgg ggaacggtca ggagaaaaaa tcggtgaagg taagcgggca tctttcctcc
    46981 aatagcggcg agattgtgct gcaatgggcg ctggagggca aagggattat gctgcgttcc
    47041 gagtgggatg tgttgccgtt tctggagagt ggcaaactgg tgcaagtatt gccagagtat
    47101 gcacaaagcg ccaatatctg ggctgtttac cgggagccgc tctatcgcag catgaaatta
    47161 cgtgtctgcg tggaatttct ggcggcatgg tgccagcaac ggctgggcaa gcccgatgaa
    47221 ggctatcagg tcatgtagat ccaccagaaa tcactcggga tccttttcgc gctttctttt
    47281 gaatttcgtc cagatttttg tctcctgacg acgccacaga cgttggatgt tgtcatgatg
    47341 acgcagcagg atcaggcaag agagcatcga aaccgggaag gtgaattgtg gcttaaacca
    47401 ccagacataa aacggagcaa tcagtgcact gacaatcgct cccagcgacg agtatccgct
    47461 caatagcacg gtcagtaacc aggttcccgc cattactccg gtgagatccc agccaatggg
    47521 tgcgatggca ccaaaagcgg tagcaacgcc ttttcctcct ttaaatccga agaaaacggg
    47581 ccagatgtgt ccaagacagg cggcgatggc aattaagcct agccagaagg ggctgacacc
    47641 taattcatac gcgccccaga cgggcaacat tcctttcaga acgtcgaaaa tcagtactgc
    47701 tacggctgct cccttgccac cgatacgtaa cacattggtt gcgcctggat tgccggagcc
    47761 gctggttcgc ggatcgggca gcccacacaa gcggcaaacc agaatggcac tggaaatgga
    47821 gccgcagagg tacgcgatga ggatcattcc aggcgcgatt gcactcataa gctgttccgt
    47881 tttgaaaatt cgtgttcaac gatgaatctg tggataatac gcacatttcg ccggaagtgg
    47941 tatccggtta gccaaaaagc aggcaggacg tgatggatat tgtatttata gagcaacttt
    48001 cggtaatcac cactattggt gtttacgact gggaacagac catcgaacag aagttagtgt
    48061 tcgatatcga aatggcgtgg gataaccgta aagcggcgaa aagtgatgat gtggcggatt
    48121 gcctcagtta cgctgacatt gcagaaacgg tggtcagcca cgtcgagggg gcgcgttttg
    48181 cgctggtgga acgcgtggct gaagaggtgg cggagctgct gttagcacgc ttcaactcgc
    48241 cgtgggtgcg tatcaaactc agcaagccag gcgcagtggc gcgggcggcg aatgttggcg
    48301 taatcattga gcgtggcaat aatctgaaag aaaataatta attttacagc tgttaaacca
    48361 aacggttata acctggtcat acgcagtagt tcggacaagc ggtacatttt aataatttag
    48421 gggtttattg atgagcgata tgcactcgct gctgatagcg gcaatattgg gtgtggtcga
    48481 aggattgaca gaatttctgc cggtatccag cacgggccat atgattattg tcggtcactt
    48541 gttggggttt gagggcgaca cggcgaaaac ctttgaagtt gtgatccagt taggatcaat
    48601 tctggcggta gtagtgatgt tctggcggcg tctgtttggc ctgattggca tccactttgg
    48661 ccgcccgttg cagcacgaag gtgaaagcaa aggtcgttta acgctgatcc acattttgct
    48721 ggggatgatt ccggcggtgg tattggggct gttgttccac gacacgatta agtcattgtt
    48781 taacccgata aatgtgatgt atgcgctggt cgttggcggt ttgttgctga ttgccgccga
    48841 atgcctgaag ccgaaagagc cgcgtgcgcc gggtcttgat gatatgacct atcgtcaggc
    48901 atttatgatt ggctgtttcc agtgtctggc gctgtggccg ggtttctccc gttccggggc
    48961 gaccatttca ggtgggatgc tgatgggggt gagccgttac gctgcttccg agttttcgtt
    49021 cctgctggcg gtgccgatga tgatgggcgc aacggcgctc gatctctaca aaagctgggg
    49081 cttcctgaca agcggcgata tcccgatgtt tgccgttggg tttatcaccg cttttgtggt
    49141 ggcgctgata gcgattaaaa ccttcctgca attgattaag cgcatttcgt ttatcccgtt
    49201 cgccatttat cgctttattg tggcggctgc ggtgtatgtc gtgttctttt aattgaggaa
    49261 tagtgtcgga tgcttggcgt tgtcaacgat tgcgtaggcc gaataaggcg ttcacgccgc
    49321 atccggcaat caacgcctga tgcgacgctt gcgcgtctta tcaggcctac aactgtcgtt
    49381 gacatgtagc cggataaggc gtttacgccg catccggcaa aatgcccaat actcattcag
    49441 gctttgggca acgttgttcc ttccagctgg ctaccgccgc aatccgccgt cgggtcagct
    49501 cctcgcgaat ctccacacct ttaaatcccg cttcaacgac ggcttttgtc ggcactgact
    49561 gcgccacttc ccaggcttcg cgcaaccagc ggccttgcgg gtagtccgct gattcaaaac
    49621 cggttctgcc gcgcacgtca gcctcgctgg tcagcgccag ttgctcgaca cgctgcggtt
    49681 tacgccaggc atcgatggaa tcaaataatt tgacgatggt ttttgggttc agcattggga
    49741 aggtgtggat gagatcgtga aactcagcca ccagtctggc taaatcgcga atttcatttg
    49801 gcacgcgcag acgctggcat agttgttcca ctaacttaac acccgccggg ccatgaccat
    49861 gatgacgcgg ccagagttct ggcggcgtca gccctttacc gagatcgtgg cataaagttg
    49921 cgaaacggac atcgacctgc ggactcagca tcgccgccat tgagagcgtc attaaggtat
    49981 gaatacccgt atcgatttcc ggatgccact tggcaggggc cggaacgcca aacagtgcgt
    50041 caatttccgg gaataaaacg cgcagtgcgc cgcaatcgcg cagtacctgg aagaacacct
    50101 gtggattgcg ggtggtgagg gcgctttccg tctctttcca tacccgttca ggcgtcaggt
    50161 gttccagttc acccgcatgg gtcatctcgc gcatcaacgc cagagtttca tcggcaatac
    50221 gaaaaccgag gtgggcataa cgcgcagcaa aacgcgccac gcgcaatacg cgtaacggat
    50281 cttcgccaaa agcgggggaa acatggcgca acagacgatt ttgcagatcg cccagaccgt
    50341 tgtacgggtc gataatctcg ccgttatcgt cctgggccag cgcattaatg gtcagatcgc
    50401 gacgcttaag atcatcttcc agcgtgacat ccggtgcggc atagcaagta aaaccggtgt
    50461 aaccggaacc ggatttccgt tcggtacgtg ccagcgcata ctcttcatgc gtttgcggat
    50521 gcagaaacac aggaaaatcg cggcctacct gctggtagcc cgcgtcgagc atctcctgtg
    50581 gcgtactgcc gaccaccacc caatctctgt ctttgaccgg tagccctaac aatgcatccc
    50641 gaacagcacc accgaccaga taaatcttca cgccatcatc ccttcgtcaa atacacaatt
    50701 cctaataata agacagtgca acgaagaagg cgatttagtt catccagcga tctttgcgtt
    50761 tgcggcttgg gatcaggtgc ggcagtacca gaccgagcag caagccaagc cccagcacgc
    50821 caccgccata cataaaccat tgcatgatga tggtgcgctg tttgtcatcc agctgtacgc
    50881 tggcggcatc gacctttttc tgcgcgacaa tcagctcgtt tttcagtttc tgattttctt
    50941 cttttaaccc gttgatcacg ctgtcgctct gcgccacttt ttgctgcatt tctgcggtgc
    51001 gctgattcca ggtgttatcg atattggtga gtttatcggt cagggttttg acctgatttt
    51061 ccagatctgg cacacgggag cgcaggcttg gctcagtgct aagttgtttc aacgggatcc
    51121 aggcggtacg gccagagctg tctttcacct gggcataatt ggtgttggcg tcagtttgta
    51181 ataaggtcac ttcctcgccg gcgttaaccg tgcccacgag gcgataatga tctcccggac
    51241 cgctgcggac ccaggtattc agttcgtcgg aaacatagcg cgtttcttca gcgtgtgaga
    51301 cggcagtcgc gctaagtgcg agtaaagtta atccgatcag gcgtaatttt ggcatcaggc
    51361 tgtcgttatt gtcatggaaa gtggaacgat agtagtggca tcagtgtcgc tacgcaaagc
    51421 attcgacatc aatcggaatc ctctgtgtca ggtcttgcca ctgcttcaaa cttttacgcc
    51481 cgtcaaattg cccgttgcat ggcaatttgg cgcaaaatac tatctactga caaaaaagat
    51541 cgcattaagt tcgccgttca tcgtcactta tgtgacataa ccgataagta aaggccatgg
    51601 ctcaggaaat cgaattaaag tttattgtta atcacagtgc cgttgaggcg ttgcgtgacc
    51661 atctcaatac gctgggcggc gagcaccatg accccgtgca gttgctgaat atttactacg
    51721 aaacgccgga taactggctg cgtgggcacg atatgggctt acgtattcgt ggcgaaaacg
    51781 gtcgctatga gatgaccatg aaagttgcag gaagagtgac aggcggctta catcagcgcc
    51841 cggaatataa cgtggcgttg agcgaaccga cgctcgacct ggcgcagtta ccgacggaag
    51901 tctggccgaa cggcgaattg cccgccgatc tcgcctcccg cgtgcagccg ctgttcagca
    51961 ccgattttta tcgcgaaaaa tggctggtgg cggtcgatgg tagccaaatt gaaatcgccc
    52021 tcgaccaggg ggaagtgaaa gcgggtgaat ttgctgaacc tatctgtgag ctggaactgg
    52081 aactgcttag cggcgacacg cgcgcggtgc tgaaactggc gaaccaactg gtatcgcaaa
    52141 ccggattacg ccagggcagc ctgagcaaag cggcgcgtgg ctatcatctg gcgcagggca
    52201 atccggcgcg tgaaatcaaa ccgaccacca ttttgcatgt tgcggcaaaa gccgatgtgg
    52261 aacaggggct ggaagcggcg ctcgagctgg cgttagcgca atggcagtat catgaagaac
    52321 tgtgggtacg cggcaacgat gcggcgaaag aacaggtgct ggcagccatt agcctggtcc
    52381 gtcatacgct gatgctgttc ggtggtattg tgccgcgtaa agcgagcact cacttacgtg
    52441 atctgctgac tcaatgcgag gcgaccattg cttctgcggt gtctgccgtg acggcggtct
    52501 actctaccga aacggcaatg gcgaagctgg cgttgaccga atggttggta agcaaagcat
    52561 ggcagccatt tttagatgcc aaagcgcagg gcaaaatcag cgactccttc aaacgctttg
    52621 ccgatatcca tctttcccgc catgccgctg aactgaaaag cgttttctgc cagccgttag
    52681 gcgatcgcta ccgtgaccag ttgccacgcc tgacgcgtga tattgactca atactgttgc
    52741 tggcgggtta ctatgatcct gtcgtcgcgc aagcctggct ggagaactgg caggggctgc
    52801 atcacgctat tgcgaccggg caacgcatcg aaattgaaca tttccgtaat gaggcaaaca
    52861 atcaggaacc gttctggttg cacagcggaa aacgttaatc aggcaaggat aaaatcgctt
    52921 atgaagccgc tctcttcacc gttacagcag tactggcaga ccgttgttga gcggctgcca
    52981 gagcctttag ccgaggaatc acttagcgca caggcgaagt cagtacttac ttttagtgat
    53041 tttgtgcagg acagcgtgat tgcgcatcca gagtggctga cggaactgga aagccaaccg
    53101 ccgcaggccg acgaatggca gcattacgcg gcatggttgc aggaggcgct ctgtaatgtg
    53161 agtgacgaag ccgggttaat gcgcgagctg cggctattcc ggcggcgcat tatggtgcgc
    53221 atcgcctggg cgcaaacgct ggcactggtt actgaagaga gcatattgca gcagctcagc
    53281 tatctggcgg agacgctgat tgttgcggcg cgtgactggc tgtatgacgc ctgctgccgc
    53341 gagtggggaa cgccgtgcaa tgcgcagggc gaagcgcaac cgctgctgat tttaggcatg
    53401 ggtaagctgg gcggtgggga actgaatttc tcctcagata tcgatctgat ttttgcctgg
    53461 ccggaacatg gttgtacgca gggcggacgc cgggaactgg ataacgcgca gttttttacc
    53521 cgcatggggc agcggctgat taaagtgctg gatcaaccaa cgcaggatgg cttcgtctat
    53581 cgcgtggata tgcgactgcg tccgtttggc gaaagtggcc cgctggtgct gagctttgcg
    53641 gcgctggaag attattacca ggagcagggg cgcgactggg agcgttacgc gatggtcaag
    53701 gcgcggatta tgggcgatag cgaaggcgtc tatgctaacg agttgcgtgc gatgctgcgc
    53761 ccgtttgttt tccgtcgtta catcgatttc agcgtgattc agtcgctgcg caacatgaaa
    53821 gggatgattg cccgtgaagt gcgtcgacgt ggtttgaccg acaatatcaa actcggcgca
    53881 ggcggcattc gcgaaattga atttatcgtt caggtgttcc agctcattcg cggtgggcgc
    53941 gaaccgtcgc tgcagtcgcg ctctttactg ccaacgctca gcgtcattgc cgcgctgcat
    54001 ctgctttctg aaaacgatgc tgaacaattg cgagtggcgt atctgttcct gcggcgtctg
    54061 gaaaacctgc tgcaaagcat taacgacgaa caaacccaga cgcttccttc tgatgagctt
    54121 aaccgtgcgc ggctggcgtg ggcgatggac tttgctgact ggccgcaact gaccggggcg
    54181 ctgaccgcac atatgaccaa tgtgcgccgg gtgtttaatg aattgattgg cgacgacgaa
    54241 agtgaaactc aggaagagtc gctgtcggaa cagtggcgtg agctgtggca ggatgcgttg
    54301 caggaagatg acaccacgcc agtgctggcg catcttagcg aggatgatcg caaacaggtg
    54361 ctaacgctga ttgccgattt ccgcaaagag ctggataagc gcaccatcgg gccgcgagga
    54421 cgtcaggtgc tcgaccatct gatgccgcat ctgctaagtg atgtctgtgc gcgtgaagac
    54481 gctgccgtta cgctgtcgcg cattaccgcc ttgctggtgg ggattgttac ccgcaccacc
    54541 tatttagaat tgctcagtga attccccgcg gcgcttaaac atttgatttc tctgtgtgcc
    54601 gcgtcgccga tgattgccag ccagctggcg cgttatccat tattgctgga tgaattgctc
    54661 gatccaaaca ccctttacca gccgacggcg accgatgcct accgcgatga gttgcgccag
    54721 tatttgctgc gcgtgccgga agatgacgaa gagcaacagc ttgaggcgct gcgtcagttc
    54781 aaacaggcgc agctgttacg catcgccgca gcggatatcg ccggtacgct accggtgatg
    54841 aaagtgagcg atcacttaac ctggctggcg gaagccatga tagatgccgt cgttcagcag
    54901 gcgtgggttc aaatggttgc ccgctacggt aagccgaatc acctgaacga acgcgaaggg
    54961 cgtggttttg cagtggtcgg ctacggcaag ctgggcggct gggagttagg ctacagttcc
    55021 gatcttgacc tgatcttcct ccatgattgc ccaatggatg cgatgactga cggcgagcgg
    55081 gaaatcgacg gacgacagtt ttatctgcgt ctggcgcaac gcattatgca cctgttcagc
    55141 acgcgaacct cttccggcat tttgtacgaa gtggatgcac ggctgcgccc gtccggggcg
    55201 gcgggaatgc tggttacctc cacagaagca tttgccgatt atcagaaaaa cgaggcctgg
    55261 acgtgggagc atcaggcgct ggtgcgtgcg cgtgtagtgt acggcgatcc gcagctcacc
    55321 gcgcactttg acgcagtacg tcgcgagatt atgacgctgc cgcgtgaagg taaaactctg
    55381 caaacggaag tgcgggaaat gcgcgagaaa atgcgcgctc atctcggcaa taaacatcgc
    55441 gatcgctttg atatcaaagc tgatgaaggg ggaattaccg atatcgaatt tattacccaa
    55501 tatctggtgt tgagctacgc tcatgaaaaa ccgaagttaa cgcgctggtc agacaacgtg
    55561 cgtattctgg aactactggc gcaaaacgac attatggaag agcaggaagc gatggcgctg
    55621 acccgtgctt acactacgct tcgcgatgaa cttcatcatc tggcattaca ggaattgccg
    55681 ggccatgtgt cggaggattg cttcaccgca gagcgtgaac tggtgcgggc aagctggcag
    55741 aagtggctgg tggaagaatg aagtatggta ttatcgcgcg caaattttga atctctcagg
    55801 agacaggaat gaaagtaacg ctgccagagt ttgaacgtgc aggagtgatg gtggttggtg
    55861 atgtgatgct ggatcgttac tggtacggcc ccaccagtcg tatctcgccg gaagcgccgg
    55921 tgcccgtggt taaagtgaat accatcgaag aacgtccggg cggcgcggct aacgtggcga
    55981 tgaatatcgc ttctctcggt gctaatgcac gcctggtcgg gttgacgggc attgacgatg
    56041 cagcgcgcgc gctgagtaaa tctctggccg acgtcaacgt caaatgcgac ttcgtttctg
    56101 taccaacgca tccgactatc accaaattac gggtactttc ccgcaaccaa cagctgatcc
    56161 gcctggattt tgaagaaggt ttcgaaggtg ttgatccgca accgctgcac gaacggatta
    56221 atcaggcgct gagttcgatt ggcgcgctgg tgctttctga ctacgccaaa ggtgcgctgg
    56281 caagcgtaca gcagatgatc caactggcgc gtaaagcggg tgttccggtg ctgattgatc
    56341 caaaaggtac cgattttgag cgctaccgcg gcgctacgct gttaacgccg aatctctcgg
    56401 aatttgaagc tgttgtcggt aaatgtaaga ccgaagaaga gattgttgag cgcggcatga
    56461 aactgattgc cgattacgaa ctctcggctc tgttagtgac ccgttccgaa cagggtatgt
    56521 cgctgctgca accgggtaaa gcgccgctgc atatgccaac ccaggcgcag gaagtgtatg
    56581 acgttaccgg tgcgggcgac acggtgattg gtgtcctggc ggcaacgctg gcagcgggta
    56641 attcgctgga agaagcctgc ttctttgcca atgcggcggc tggtgtggtg gtcggcaaac
    56701 tggggacgtc cacggtttcg ccgatcgagc tggaaaacgc tgtacgtgga cgcgcagata
    56761 ccggctttgg cgtgatgacc gaagaggaac tgaagctggc tgtagcggca gcgcgtaaac
    56821 gtggtgaaaa agtggtaatg accaacggtg tctttgacat cctgcacgcc gggcacgtct
    56881 cttatctggc aaatgcccgc aagctgggtg accgcttgat tgttgccgtc aacagcgatg
    56941 cctccaccaa acggctgaaa ggggattccc gcccggtaaa cccactcgaa cagcgtatga
    57001 ttgtgctggg cgcactggaa gcggtcgact gggtagtgtc gtttgaagag gacacgccgc
    57061 agcgcttgat cgccgggatc ttgccagatc tgctggtgaa aggcggcgac tataaaccag
    57121 aagagattgc cgggagtaaa gaagtctggg ccaacggtgg tgaagtgttg gtgctcaact
    57181 ttgaagacgg ttgctcgacg accaacatca tcaagaagat ccaacaggat aaaaaaggct
    57241 aaccggaaag cggttcacag atctcgcttc gtacatggca ggcctattga cgctgttgtt
    57301 ttttcaacct agagtaaagg aacaagggta agggaggatt tctcccccct ctgatgagtt
    57361 gttagtaagt cgggaaactt aacagtaaca acacaaccag tatgatgacg aacttcatca
    57421 taaccctttc cttatacaag gccccttctt cgggaggggc tttcccgttt cagccccttg
    57481 ctgccccccg attgttgatg agcgccggac cccgcgcaat gctttcccag aggcaatttt
    57541 gccttccccg agcggtcacg caaaacgctg caacgtcctg agcatttgca ccttttagcg
    57601 gaacgcagat ataaaaaatc cccggctttt tgggccaggg atatgtgtta atgaaacctt
    57661 cttgacgctg attttttttc aacctaaagt aaaggaacaa gggtgaggga ggatttctcc
    57721 cccctctgat tggctgttaa taagctgcga aacttacgag taacaacaca atcagtatga
    57781 tgacgagctt catcataacc ctttccttct gtaaggcccc ttcttcggga ggggctttcc
    57841 cgtttcagcc ccctgctgac tccccgattg ttgatgagcg ccagacccca cgcaatgctt
    57901 tctctgatgc agttttgcct tccccgagcc gtcacgcaaa atgctgcaac aacctgatca
    57961 tttgcacttt ttagcggaac gcagatataa aaaatccccg gctttttggg ccagggatta
    58021 ttcctggaag agggtttact ctgctttttc ttcgacggga gttgttgagg ttaagggtga
    58081 agtcaatgcc gccagtgagc cgcctgaaac gccaatttca ttgagcaagg agtcaatcag
    58141 cggtgcctgt gtgcggtaag agagagcggc tgataatgct tgttccgcca ggtttccgcc
    58201 ccccacatta cccgtgtttg catcacccgc agcgccgcca cgattcagac catcgacctg
    58261 aataatcttg ataccgtcga ttgacttcat cggctcaacg gatttctcta ttaccgcagg
    58321 cagcgcctgc aacaaggcca gtttgaattt aaggctggtt tgttcatcag aaagtacgtt
    58381 gatagcatcg ttcagcgcac gttgtgcttc tgcttccgcc agaccctttt tacgtgtagc
    58441 ttcggctaac tcaacgatag ccgccgcctg catctctgcg gcttcttttt ctgctttcgc
    58501 ccgcacggtc agttcaaccg ctttggtttc tgcatcctgc gcggcagcga ttagggcaac
    58561 ttgtttagca cgatcggctt cggcagtctg gcgagtggtt tctacgtttt gttgggcgct
    58621 taccgcttct gcaagtgcga gattagcacg cgcttctgcc tgggactgtt gttccgattt
    58681 ggcggcaata gcgatcgatt tcgtctggtt agcgatttcg gtgacctgct gctgttcgat
    58741 ctctttaatg cgaacttcac gttcagcttc aacctttctt gagcggacgg cctgttcgcg
    58801 gtcgatttct gtttcctgaa tctgtcgttc agccagaatt cgtgtctgct ctgcttcacg
    58861 acgacgttca gcttcaaaag ccgcaatacg tgcattctgt tcggcagtac gggttttaac
    58921 ctgctgctcc tgctcaagcg tcataaacgc ttcttgctgt tcaatctcca gcttgcgcga
    58981 tagcgcatcg cgattttttt cacgcaccgc aacttctaca tcctgttcaa cttcgttacg
    59041 ttcgcggcga cggcgctctg tttcctgagt cagtttggtt aaaccttcgg cgtcaaaggc
    59101 attgttcgga ttgaaatgtt ctttcgaggt ctggttaaag ttggtaagtg aaacgctctc
    59161 cagttccaga ccgtttttcg acaggtcttc tgccactgta ttttgcaccc cctgcacaaa
    59221 gttctcgcgg gtatcctgta actcatgcat ggtcatttgc gcagctgttg cacggagggc
    59281 atcgacaaat ttatcttcaa ccaacatacg taagtcttca ggcgacaggg tgcgttgccc
    59341 cagcgtctgg gcagcggtgg caatcccttc tactgaaggt tttacccgca caaagaaagc
    59401 gactactaca tcgacgcgca tacgatcttt cgtaatcagg ctatcaatgg ttgagcggct
    59461 gacttccagc ttcagagtat tcatattgat ggggattatt tcatgaaaga tcggcatcac
    59521 gattgcgcca ccgctcatta ccactttttg cccacctaaa ccagtacgaa caaaagcttg
    59581 ctctgccgaa gcgcgacgat agagcctggc gaaaataatt ccaataataa acagaatgca
    59641 tacggcaata attgcggtaa acatccagga gggcacagaa ttaacaatat catccattat
    59701 taaatttcct tttgattaat aaattacaat atttggggcc agggattatt ttccgccaaa
    59761 tatcgcgttg cagaaagtcg gcaaataatt aacactttgt ctcctttagt gaaaattttt
    59821 ccttcttcag gctccagtag taaataatgt atttggccaa attgatcggt gagttttcct
    59881 tcgcagggat tacctgaagt ggcttgatga ccggtaatta acgccatgct accaatatat
    59941 tcttcttctg taatagctga actatggtcg cgtggaatcc agggcgcaac tattttaccg
    60001 gtataatgca ccgcaataat cgtaaacagt aaactgacag gaaccacgaa caggttcgac
    60061 agtggtgatt gccagaccat gatgcaggca tgctgcaaca ggatgccaat aagaccaaaa
    60121 aaaccagcca gtaagcagag aacgacgaga gctggcaatc ttccaatatt gagataatga
    60181 agtgcctggc taatatgacc tgtggttata gaatcataat ggtcaagatg tgcatcaaga
    60241 gcaccggaga gcatgtgacc gcagataagc gcaaaaattt ctaacaggcc gatcaacagt
    60301 acaaaggaaa tagcgaaaag ataaggggtg ttatagtcgg cgaataaaat catatatcac
    60361 tccaatgtcc atttagtgat gcaatattta ctttgtaata gtttccttgc ttacatttat
    60421 ctgtgttaat aataacacag gttagagaag ttctggaaga gaaagtcttt taatatggtt
    60481 ttttattttt cactaaaaag tgtgatcggg gacaatatat ttacgcacgt tatgtttaaa
    60541 ggcactacac tgattgggga atactgaaat cagaaaaagc atatggcctt aaaaggtatg
    60601 cgataaccgg aggatgtgct tatggatcat agtcttaatt ctttaaataa tttcgatttc
    60661 ctggcgcgta gttttgccag aatgcacgca gaaggtcgcc cggtcgatat tctggccgtt
    60721 actggtaaca tggatgaaga acatagaacc tggttttgcg cacgttatgc ctggtattgt
    60781 caacagatga tgcaggcaag agagctggag ttagagcact gatataacgg gcctgatggc
    60841 ccgttttagt gtttgtatta gatactagtt ataggtgatt ttcaggatta cgtctttact
    60901 aaacggtccc tcaacaaccg tttctgccgg gttcgcactc agttctgccc tgaatggtgc
    60961 ggtgaccgaa ggcgcactag acgatgaaaa agaagcaaat tgtgagaagc ggttaaattc
    61021 cgttgctgtt ccatcgttat gaaatatctt caatagcaga ccattaccta tatccagatg
    61081 gctataatca ctcaaaatat ccgtagtgaa aaaactccct aaaatattaa actgctcagt
    61141 acaggccgca cccggatctt tggtcatgac tacgctaaac gtctcactca cgctggtatt
    61201 tttaattgtt cgggaattta ttttaccaaa gtcgacaacg ccatcagctg gttccagatc
    61261 aacattaaca aagcagtcaa ggacatgaat gttatttaat ccgcgaatcc tgaaggtcag
    61321 atttttatct acttctatag cacctgctcc gctcatacct ttaaattcaa gtgctatata
    61381 ttcgtcaggt aatacaagtt ttttatttac tttattggca tttttagcta gcttaatata
    61441 tagctgcact cgtactggaa aagttactga taattgcggt tcaggaccgc cactacagac
    61501 tgcgggcttt ttgtagccat tcccgaaggt tccggctcct tttatatcaa tacatacagg
    61561 tgctgcaata ggtagtggag atgtagaatc ataatcaacc ccattataag ttacaccgaa
    61621 agtataagag ttttgattat aaaagtcttg acctgatgct aacaacatca ttatatttgc
    61681 ccatgcagat gggtattctt ctcctggaga tgggcctggt agtgcattat tacagaaaac
    61741 attcataaaa tattcactgc tttgccagat tttatcacct tttttaccat cgctggggat
    61801 agagattctt ggtacgtaac caatgacaac ggtatttcca ccttcatgtt cagcatagca
    61861 gtccattgcc catgcaggta atgaactaaa acccataaaa aaagtaataa caattaacaa
    61921 gtagcgcata ttattgatct cggttggcta cagaaatatt acaaatgtta tttactacag
    61981 ggcagtttat ttcataaaaa cttaatccac caaagtcatt gatataaccc acctgaaaag
    62041 ttgacggaac atttttaaac tgcgtatttg catagctaaa aggggttacc attaagcttt
    62101 gcgtttgtgg taacatcgtt accccatttg ttccgatata acccagcgta acgtgataag
    62161 gcatcttatt gttaaagcgg atcgaaccgt catttctacg aatgatttca actttttcgt
    62221 aagtcattac cccatcgtct tctaattcaa tggcttttgg ccgccagaat agtttcaatg
    62281 cgtgctgcat agtgacttgc atcacatttt tttatctgtt ttgggcggta tttctcgcac
    62341 gttaaaatag aagagcgttt ctctgtcttt aggaagttga gcaatttttt cctgagttat
    62401 cattcttatt tgaatttgct cactgggatt taaacgcacc attggaggaa cagtgacaat
    62461 aaagtcacgc gatttattgc ctttcgtatc ttctatccat gtttgggcca gatagggcag
    62521 gtttttactt tgattagtaa gttttaatgc ttcgccttta tctgactcat tcattatgat
    62581 acgggtacga tccgggatga tggcacccca ggcaatgctg ctcatacagg agagtaatac
    62641 tcctgctgca atgatattct ttgtatttaa gtagcgcatt agtttcatcc tgaattaatt
    62701 ttggctaata cacggtaaaa tagccgttcc ggtggttaag ttgctgctat tgggtggttg
    62761 aataaaacaa gatttgtcac cccagcgtaa agcaagtttg ttatcatcct gaatgccgtt
    62821 gaggtataca aagccgccat cagcaaccag tcctaattct ttgtggctgg tttcatcttt
    62881 tacgctaatc cccagtggtg gatgactacc atctgccagg cgaataaccc ctatgatttg
    62941 gtagcccttg cgggtatcaa gtttggcgta gccaatagca ccctcggtta aggtggtggt
    63001 gataaccgag ttttcgatat ccacattttc tggcagattt cgggtatcaa cacttaatga
    63061 tgttgttatg taactgctga ctgatggaat aactccgatg ccataccgat tagttaccgc
    63121 gcgtttattg ttcaacggaa tatcttcagt gccgtctgcg tcgatcataa aacgcgagtc
    63181 agcactaccg ctatagtcgt ggaaagcggc accgtggcga gtcgcagtga atgaaccatt
    63241 ccaactggca ttcagagaat aataactgtc tcgctgtgat gtaccggaga ggtagagtcg
    63301 accgttctcg gtattatgct gataactggc gcgtagtgac gctttcatat ctttgaattc
    63361 atcattgtcg cctgaagcag aaacgctcca ggaattattt cgatcggaag agtcatacca
    63421 cgaagcagta tgcgaaatct cattatcctg atttcgttgc ataccatagc tcaatgttct
    63481 actagttccc cagggaatcg aaatatttag gtacagttga tcctggttgt cttcttccca
    63541 gtttattcgg ctaaatgtta gagaagtgga gacgtttttt aatggcccga tatcaaaatc
    63601 gcggctaagt gataatgaat aattcacatt actgctggcg tcccagtaag tattacgagc
    63661 caaacttacg tatgtattta accttaatga ttcaagatac tggttaaacg tgacaatata
    63721 actctctttt tcattctgat aattcgtgta atggttagtg tcattgatat attcattcat
    63781 tgtcacaaag tttttatcag agaaacggta accagcgaaa gtgagctggc taccggtaga
    63841 ttcaaaacgt tttgaatagt tagcgcggta gctataaccc gtttctttat cctgattatg
    63901 caactgagca tcagatcgtg tgacatcaaa agataatgaa cctaatgagt taaggttaaa
    63961 accaacgccg gcagccagag attgataatc acggtttgtt aaaactgaac caccatacag
    64021 ggatacattg ttcagccaac cccatgagaa ttcaccagtc cagaagaagg gttgttgtaa
    64081 ggaatcacca ccaacgctgg tcggtttacc catagcagtg gtgtagcgaa tctgcccttt
    64141 acgcgtcaga taaggaatac tattagatgc aacttgccat tgcgtaacgc gtccgtcctc
    64201 ttcttcaacc ttgacgtcaa gttgcccctg taatgtattg aataaattgg taatagcaaa
    64261 tgggcctgga ggaacatttt cttgataaat aatacggttg ttctgtgaga cagtgacctt
    64321 tgcattcgtt tgtgcgacac ccgtgatttg cggtgcgtaa ccacgtaggt ctggcggtaa
    64381 catattctca tcgcttttca acgaaatgcc agaaaaatgg aaagagtcga aaatagagga
    64441 ttcagtatcg tattgaccta tagttagttt tgcattcatt gctgggattg ggcgaaacaa
    64501 ataaatacgc ggaaaatcca ggttggtcgc ctggcttttg ccattgttaa atgactgatc
    64561 gtactggtaa tcagtacgca ggcgccaggc cccaagatta accccagcct gcccatagga
    64621 acttatattt tgcgaattat cgccctgatg tggtgcataa tgagaggcat ataaattgta
    64681 gtcaagaaat gccccagcaa taccatgatt ccaaagttca ggaggcgtcc agtttgcatc
    64741 tttgtatttt aaccatgcct gaggtgcaga tatggataat tgcattttcc ctttatcgag
    64801 ataagttgta atttgttttt ctttttcgat agggtaacaa ccatcgatca ggttttgctg
    64861 agttttttca ataaattcat cagtaaatcc taataatgtt aattgctctg gtgtcacgca
    64921 aactgcagtt tggttattaa ttaataccca ttggaggtca aggtggattg gcgtcctctt
    64981 attattaatc agagtgctta gtgaatattt gccgacgggt aattggccat cagtttcaaa
    65041 ttgggaaagg ttaacgtttt cacgatcttc ggcttcgatt aaatctttgt tgaattcaac
    65101 ggcataagct gacgcacaac caatgatgat aacggataat gaacaataaa tatttttaat
    65161 caattcgctt attgtggtta attttaattt tttatacatc tgatccactt atcattaatc
    65221 cgttaatgat gtggccgggc gaacccggcc aaatgcgatt actcgtaagc aatcttgtag
    65281 tcagcctgag cgtaaacgtt gcccggggtg gcatctttgc ttggggattc catacgagct
    65341 ttgaaattca gttcctgata gctgttggta ttgttgaacg taattactac cggagtacct
    65401 aaagttactt cgccgctctg gctgtccaga atacgtacgc caacgccagt ggcgccgccg
    65461 atttcagttt ctttggtatt tttcagaaga tcagactgac ccgtcatggt atttgctgta
    65521 gtgaaagtaa ccttcgcttt tgaataagta gttccaccct gactcagcat gcagtcctgc
    65581 aggtgaatag tgaaaggttc agccaaagat ttctgatcgc ttttcagtac ggtatctgct
    65641 acttcaccca gatttacagt taaatcttcg tcacctggtt taatagaaca tggagcagag
    65701 ataacttcac ctttaaattt aacaataccg tgaccttcat cagcaaacac gttattggaa
    65761 agaatcattg ctacgccagc aacaagaagt gactttttaa aagcggacat aatattcctt
    65821 tatataaaga taagtagaaa agagaatgat tagtaactgt tatttaagga ctactaatca
    65881 ttgaaatatg atattttatt tt
//
LOCUS       DAZFZC010000014        65414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFZC010000014 DAZFZC010000000
VERSION     DAZFZC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.014"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..469)
                     /gene="folK"
                     /locus_tag="ACHKXP_001064"
     CDS             complement(<1..469)
                     /gene="folK"
                     /locus_tag="ACHKXP_001064"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO7646120.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVLQT"
     gene            complement(466..1863)
                     /gene="pcnB"
                     /locus_tag="ACHKXP_001065"
     CDS             complement(466..1863)
                     /gene="pcnB"
                     /locus_tag="ACHKXP_001065"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO7646121.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1923..2819)
                     /gene="gluQRS"
                     /locus_tag="ACHKXP_001066"
     CDS             complement(1923..2819)
                     /gene="gluQRS"
                     /locus_tag="ACHKXP_001066"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO7646122.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGLWLVRI
                     EDIDPPREVPCAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2886..3341)
                     /gene="dksA"
                     /locus_tag="ACHKXP_001067"
     CDS             complement(2886..3341)
                     /gene="dksA"
                     /locus_tag="ACHKXP_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO7646123.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3519..4223)
                     /gene="sfsA"
                     /locus_tag="ACHKXP_001068"
     CDS             complement(3519..4223)
                     /gene="sfsA"
                     /locus_tag="ACHKXP_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO7646124.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGVMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4238..4768)
                     /gene="thpR"
                     /locus_tag="ACHKXP_001069"
     CDS             complement(4238..4768)
                     /gene="thpR"
                     /locus_tag="ACHKXP_001069"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO7646125.1"
                     /translation="MSDPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4842..7271
                     /gene="hrpB"
                     /locus_tag="ACHKXP_001070"
     CDS             4842..7271
                     /gene="hrpB"
                     /locus_tag="ACHKXP_001070"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO7646126.1"
                     /translation="MSLLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGLSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRISQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPAVNLLAAKRL
                     LKMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDEFHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7404..9938
                     /gene="mrcB"
                     /locus_tag="ACHKXP_001071"
     CDS             7404..9938
                     /gene="mrcB"
                     /locus_tag="ACHKXP_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO7646127.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRNRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10158..12407
                     /gene="fhuA"
                     /locus_tag="ACHKXP_001072"
     CDS             10158..12407
                     /gene="fhuA"
                     /locus_tag="ACHKXP_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO7646128.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSDRDD
                     KQFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVGSYT
                     YTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            12458..13255
                     /gene="fhuC"
                     /locus_tag="ACHKXP_001073"
     CDS             12458..13255
                     /gene="fhuC"
                     /locus_tag="ACHKXP_001073"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO7646129.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13255..14145
                     /gene="fhuD"
                     /locus_tag="ACHKXP_001074"
     CDS             13255..14145
                     /gene="fhuD"
                     /locus_tag="ACHKXP_001074"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO7646130.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPLLPESVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14142..16124
                     /gene="fhuB"
                     /locus_tag="ACHKXP_001075"
     CDS             14142..16124
                     /gene="fhuB"
                     /locus_tag="ACHKXP_001075"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO7646131.1"
                     /translation="MSKRIALFPALLLALLVIVAMALTWMNFSQALPRSQWAQATWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAMGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16159..17439)
                     /gene="hemL"
                     /locus_tag="ACHKXP_001076"
     CDS             complement(16159..17439)
                     /gene="hemL"
                     /locus_tag="ACHKXP_001076"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO7646132.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAGSVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17605..17652
                     /gene="yadX"
                     /locus_tag="ACHKXP_001077"
     CDS             17605..17652
                     /gene="yadX"
                     /locus_tag="ACHKXP_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO7646133.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17664..19085
                     /gene="clcA"
                     /locus_tag="ACHKXP_001078"
     CDS             17664..19085
                     /gene="clcA"
                     /locus_tag="ACHKXP_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO7646134.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLGRDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19109..19174
                     /gene="yadW"
                     /locus_tag="ACHKXP_001079"
     CDS             19109..19174
                     /gene="yadW"
                     /locus_tag="ACHKXP_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO7646135.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19167..19511
                     /gene="erpA"
                     /locus_tag="ACHKXP_001080"
     CDS             19167..19511
                     /gene="erpA"
                     /locus_tag="ACHKXP_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO7646136.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19558..20181)
                     /gene="yadS"
                     /locus_tag="ACHKXP_001081"
     CDS             complement(19558..20181)
                     /gene="yadS"
                     /locus_tag="ACHKXP_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO7646137.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20219..21019)
                     /gene="btuF"
                     /locus_tag="ACHKXP_001082"
     CDS             complement(20219..21019)
                     /gene="btuF"
                     /locus_tag="ACHKXP_001082"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO7646138.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21012..21710)
                     /gene="mtnN"
                     /locus_tag="ACHKXP_001083"
     CDS             complement(21012..21710)
                     /gene="mtnN"
                     /locus_tag="ACHKXP_001083"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO7646139.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21794..23311
                     /gene="dgt"
                     /locus_tag="ACHKXP_001084"
     CDS             21794..23311
                     /gene="dgt"
                     /locus_tag="ACHKXP_001084"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO7646140.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPTIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23441..24865
                     /gene="degP"
                     /locus_tag="ACHKXP_001085"
     CDS             23441..24865
                     /gene="degP"
                     /locus_tag="ACHKXP_001085"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO7646141.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25020..26177
                     /gene="cdaR"
                     /locus_tag="ACHKXP_001086"
     CDS             25020..26177
                     /gene="cdaR"
                     /locus_tag="ACHKXP_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO7646142.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26266..26652)
                     /gene="yaeH"
                     /locus_tag="ACHKXP_001087"
     CDS             complement(26266..26652)
                     /gene="yaeH"
                     /locus_tag="ACHKXP_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO7646143.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26966..27790)
                     /gene="dapD"
                     /locus_tag="ACHKXP_001088"
     CDS             complement(26966..27790)
                     /gene="dapD"
                     /locus_tag="ACHKXP_001088"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO7646144.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27821..30493)
                     /gene="glnD"
                     /locus_tag="ACHKXP_001089"
     CDS             complement(27821..30493)
                     /gene="glnD"
                     /locus_tag="ACHKXP_001089"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO7646145.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30555..31349)
                     /gene="map"
                     /locus_tag="ACHKXP_001090"
     CDS             complement(30555..31349)
                     /gene="map"
                     /locus_tag="ACHKXP_001090"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO7646146.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31717..32442
                     /gene="rpsB"
                     /locus_tag="ACHKXP_001091"
     CDS             31717..32442
                     /gene="rpsB"
                     /locus_tag="ACHKXP_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO7646147.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32577..33428
                     /gene="tsf"
                     /locus_tag="ACHKXP_001092"
     CDS             32577..33428
                     /gene="tsf"
                     /locus_tag="ACHKXP_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO7646148.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33575..34300
                     /gene="pyrH"
                     /locus_tag="ACHKXP_001093"
     CDS             33575..34300
                     /gene="pyrH"
                     /locus_tag="ACHKXP_001093"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO7646149.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34592..35149
                     /gene="frr"
                     /locus_tag="ACHKXP_001094"
     CDS             34592..35149
                     /gene="frr"
                     /locus_tag="ACHKXP_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO7646150.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35241..36437
                     /gene="ispC"
                     /locus_tag="ACHKXP_001095"
     CDS             35241..36437
                     /gene="ispC"
                     /locus_tag="ACHKXP_001095"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO7646151.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36626..37384
                     /gene="ispU"
                     /locus_tag="ACHKXP_001096"
                     /gene_synonym="uppS"
     CDS             36626..37384
                     /gene="ispU"
                     /locus_tag="ACHKXP_001096"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO7646152.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37397..38254
                     /gene="cdsA"
                     /locus_tag="ACHKXP_001097"
     CDS             37397..38254
                     /gene="cdsA"
                     /locus_tag="ACHKXP_001097"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7646153.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38266..39618
                     /gene="rseP"
                     /locus_tag="ACHKXP_001098"
     CDS             38266..39618
                     /gene="rseP"
                     /locus_tag="ACHKXP_001098"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO7646154.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39648..42080
                     /gene="bamA"
                     /locus_tag="ACHKXP_001099"
     CDS             39648..42080
                     /gene="bamA"
                     /locus_tag="ACHKXP_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO7646155.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42202..42687
                     /gene="skp"
                     /locus_tag="ACHKXP_001100"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42202..42687
                     /gene="skp"
                     /locus_tag="ACHKXP_001100"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO7646156.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42691..43716
                     /gene="lpxD"
                     /locus_tag="ACHKXP_001101"
     CDS             42691..43716
                     /gene="lpxD"
                     /locus_tag="ACHKXP_001101"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO7646157.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAEIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43821..44276
                     /gene="fabZ"
                     /locus_tag="ACHKXP_001102"
     CDS             43821..44276
                     /gene="fabZ"
                     /locus_tag="ACHKXP_001102"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO7646158.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44280..45068
                     /gene="lpxA"
                     /locus_tag="ACHKXP_001103"
     CDS             44280..45068
                     /gene="lpxA"
                     /locus_tag="ACHKXP_001103"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO7646159.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45068..46216
                     /gene="lpxB"
                     /locus_tag="ACHKXP_001104"
     CDS             45068..46216
                     /gene="lpxB"
                     /locus_tag="ACHKXP_001104"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO7646160.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGAREVLGIPHDTHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTEYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46213..46809
                     /gene="rnhB"
                     /locus_tag="ACHKXP_001105"
     CDS             46213..46809
                     /gene="rnhB"
                     /locus_tag="ACHKXP_001105"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO7646161.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVITAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGVTEHHRRSFGPVKRALGLAS"
     gene            46846..50328
                     /gene="dnaE"
                     /locus_tag="ACHKXP_001106"
     CDS             46846..50328
                     /gene="dnaE"
                     /locus_tag="ACHKXP_001106"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO7646162.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50341..51300
                     /gene="accA"
                     /locus_tag="ACHKXP_001107"
     CDS             50341..51300
                     /gene="accA"
                     /locus_tag="ACHKXP_001107"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO7646163.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51399..53540
                     /gene="ldcC"
                     /locus_tag="ACHKXP_001108"
     CDS             51399..53540
                     /gene="ldcC"
                     /locus_tag="ACHKXP_001108"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO7646164.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53597..53986
                     /gene="yaeR"
                     /locus_tag="ACHKXP_001109"
     CDS             53597..53986
                     /gene="yaeR"
                     /locus_tag="ACHKXP_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7646165.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54051..55346
                     /gene="tilS"
                     /locus_tag="ACHKXP_001110"
     CDS             54051..55346
                     /gene="tilS"
                     /locus_tag="ACHKXP_001110"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO7646166.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRMRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GLGSVQLNAGGAIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55399..55659)
                     /gene="rof"
                     /locus_tag="ACHKXP_001111"
     CDS             complement(55399..55659)
                     /gene="rof"
                     /locus_tag="ACHKXP_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO7646167.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55646..55846)
                     /gene="yaeP"
                     /locus_tag="ACHKXP_001112"
     CDS             complement(55646..55846)
                     /gene="yaeP"
                     /locus_tag="ACHKXP_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO7646168.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56012..56557
                     /gene="yaeQ"
                     /locus_tag="ACHKXP_001113"
     CDS             56012..56557
                     /gene="yaeQ"
                     /locus_tag="ACHKXP_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO7646169.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56554..56976
                     /gene="arfB"
                     /locus_tag="ACHKXP_001114"
     CDS             56554..56976
                     /gene="arfB"
                     /locus_tag="ACHKXP_001114"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO7646170.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56990..57700
                     /gene="nlpE"
                     /locus_tag="ACHKXP_001115"
                     /gene_synonym="cutF"
     CDS             56990..57700
                     /gene="nlpE"
                     /locus_tag="ACHKXP_001115"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO7646171.1"
                     /translation="MVKKAIVTAMAVINLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGKPIESQFNYTLEAAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57901..58725)
                     /gene="yaeF"
                     /locus_tag="ACHKXP_001116"
     CDS             complement(57901..58725)
                     /gene="yaeF"
                     /locus_tag="ACHKXP_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO7646172.1"
                     /translation="MGKPKAYYRLLLPSFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58778..60496)
                     /gene="proS"
                     /locus_tag="ACHKXP_001117"
     CDS             complement(58778..60496)
                     /gene="proS"
                     /locus_tag="ACHKXP_001117"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO7646173.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIKK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60607..61314)
                     /gene="tsaA"
                     /locus_tag="ACHKXP_001118"
     CDS             complement(60607..61314)
                     /gene="tsaA"
                     /locus_tag="ACHKXP_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO7646174.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDACASYAQSAPAAE
                     MTVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRITDAGFEVFALEPR"
     gene            complement(61311..61715)
                     /gene="rcsF"
                     /locus_tag="ACHKXP_001119"
     CDS             complement(61311..61715)
                     /gene="rcsF"
                     /locus_tag="ACHKXP_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO7646175.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61833..62648)
                     /gene="metQ"
                     /locus_tag="ACHKXP_001120"
     CDS             complement(61833..62648)
                     /gene="metQ"
                     /locus_tag="ACHKXP_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO7646176.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62688..63341)
                     /gene="metI"
                     /locus_tag="ACHKXP_001121"
     CDS             complement(62688..63341)
                     /gene="metI"
                     /locus_tag="ACHKXP_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO7646177.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63334..64365)
                     /gene="metN"
                     /locus_tag="ACHKXP_001122"
     CDS             complement(63334..64365)
                     /gene="metN"
                     /locus_tag="ACHKXP_001122"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO7646178.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64553..65128
                     /gene="gmhB"
                     /locus_tag="ACHKXP_001123"
     CDS             64553..65128
                     /gene="gmhB"
                     /locus_tag="ACHKXP_001123"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO7646179.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15417 a        16277 c        18064 g        15656 t
ORIGIN      
        1 aggtttgtag tactgctagc gaagtgagta tttcacgcaa cgtctcccca tcaggaaaca
       61 ccaactccgg cgcgatttca aacagcggcc acagcataaa tccacgattt ttcatatcgt
      121 agtgcggaac ggtcagtcgt tcagtattta tcacttcatt accaaacagc atgatgtcga
      181 gatccagcgt gcgcggtccc cagcgttcag ctttacggac gcgaccttgc tgcaattcaa
      241 tacgctgtgt gtgattgagt agctcttcag gtgcaagaga ggtttccagc gccacggctg
      301 cgtttaagta atcgggttga tcttgcggcc ccagcggtgg agtgcggtaa aacgaagaaa
      361 cggcaagaat gtggctttca gggatatcgc ctaatgcttt cagggcagca ttgacctgct
      421 ccagcggaga ggccagattg ctgcctatgg caatatacgc cactgtcatg cggtaccctc
      481 acgacgtggt gcgcgtttgc gtggacgacg agtacgacga cgcggtgacg gttcttcatc
      541 cagctcgttg agcatccctt tttggtctgg tggcgcggaa acctggaact caccccacca
      601 tttcaccaga cgctgcagtt cagcgttacg ttcaacttca gctcgcaagg ccaacaggtc
      661 ataagccgca cggaacttag gatgctccag cagtttccat gcgcgtttac cctgacgtcg
      721 ggacatacgc aactgcaact gccagatatc gcgggttaac gtcgtcagac gtttcgggat
      781 tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc gccagcgcga aagcgtcgtg
      841 ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc tccagcagtg ggtaccagaa
      901 catggcggca aacaggaacg ccgggttcac gcgcatatcg ttatggatac gcgtatcggt
      961 attcttcagc acctgttcaa tgatccgctc catcgggctg tcgccatttt ccgtgaagta
     1021 gcgggtaatg gtcgggaaca gcggctggaa cagatgatat tcacacaaca gcttataggt
     1081 ttcgtaaccg tagcccgctt gtagcagttt aagcgattct tcaaacaggc gtgccggtgg
     1141 gatatcgttc agcagggtag cgaggcgagg gatcggttct gcggtttccg ggctgatgcg
     1201 catacccaat ttggcggcaa aacgtactgc gcgcagcata cgtaccggat cttcacggta
     1261 gcgcgtttcc gggttaccaa tcagacggat aacaccgtcc ttcagatcct tcataccgcc
     1321 aacgtaatca cggacggtaa aatccgctac gctgtaatac aggctgttga tagtgaaatc
     1381 gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt
     1441 ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt
     1501 cgcaacttcg ataatctccg ggccaaacat cacatgagcc agacggaaac ggcgacctac
     1561 caggcggcag ttacggaaca gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc
     1621 aaagtctttc ggctttttgc caagtaacag gtcgcgcacg ccgccgccaa ccagccaggc
     1681 ttcgtatccc gctttattga gcctgtacat taccttcagg gcattttcac tgatatcttt
     1741 gcgggaaata gcatgctgct cacgcgggat caccgtcacc tgtggacggg cgactgcctg
     1801 ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa
     1861 aatagtacac ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg gctaatcata
     1921 gctcagcatg acgcatttga gaatgttgaa tttacaattg ccgactcagg cactgcggta
     1981 agccgccagt ttttgacggc tgactgaagg atttgctcga cgctgaaatc ctgccagtgt
     2041 gcttctgcct gctgccccag aaattgaagt gccgcgatta gtaccgggcg tggatcgcct
     2101 ttcggcaacg caggcgcatg attctgcttg gaaagtttag cgccttgtgg attaagcgcc
     2161 agcggcagat gaatgtaatc tggcactttc cagccaaaaa gctggtacag cgagatttgc
     2221 cgcactgtcg gttcgatcaa atctgccccg cggacaattt cgctcacgcc ctggaagtga
     2281 tcatccacca ccaccgccag gttataggca aacaaaccat cacggcgatg aataataaaa
     2341 tcttcccgtg ccagtttttc gtcggcgtga ataatgccgc gcagctggtc agtaaattgc
     2401 gtgaccggat gctgctggcg gatacgtact gcggcgttgt ctggtccatg atgcaattcc
     2461 cggcaatgac cgtcgtaaat accgccaatg ctttgaatac gtgcacgcgt gcaggtgcag
     2521 taataactta gtccttgctc atgtaaccaa gcgagtgctt cgcgataggc gtggtgacgt
     2581 tgcgattgcc agagaacatc gccgtcccag tgcaggccgt aatgttccag ctggcgcagg
     2641 atagtttctg cggcacaggg aacttcacga ggcgggtcga tatcttctat gcgtaccagc
     2701 cagagacctt gccgggcgcg agcctgcaaa tagctgccaa gcgcagcaat cagagaacca
     2761 aaatgaagct cgccggaagg agagggggcg aagcggccaa tatagtgtgt gtctgtcatc
     2821 tctttgaaca aaaaataagg cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc
     2881 tgtaattagc cagccatctg tttttcgcga atttcagcca gcgttttgca gtcgatgcac
     2941 agatcggctg tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc gcaggattca
     3001 cagtagccga aatcttcgtc ttccactttt ttcagcgtct tctcgatctt tttgatcagc
     3061 ttacgctcgc gatcgcggtt acgcagttcg aggctgaact cttcttcctg ggctgcacgg
     3121 tctaccgggt ccgggaagtt ggctgcttca tcctgcatat gtgtaacggt gcgatcgact
     3181 tcatccctga gttgattacg ccatgcttcc agaatacgac ggaagtgcgc cagctgggct
     3241 tcattcatat actcttcgcc cggcttctcc tgatatggtt ccaccccagc gatggcgaga
     3301 atactcaggg acgatgtttt acggttttgc ccttcttgca tgttgcttct ccttaacacg
     3361 cactatcgat ccccatgttc gggggaaaaa tgaggccgct ataaatagca gatgcttttc
     3421 cggatagcaa ttatctaaac gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc
     3481 acgcgaccag aatgtaaatt aaccagttac ttactttact acaatgtaac cggcagtgat
     3541 tttttaagag ccatgccttc agcagaaatt tccgctttgt aagccagaat ttctaccccc
     3601 ctctgttgag cttctgacaa tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa
     3661 aaccgtgtaa tggctgaatg cagcacggcg aaaaagataa ccgcacgctg gccttcagcc
     3721 gctacgctca tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa
     3781 tatccctgtt cgttctccgc taacgtaacc gatttcactt caatatagca gtctggacgc
     3841 gaatccgcct gcaacataaa gtcaatacgg ctgcgttctg cgccgtattt tacttcgctt
     3901 ttcagcgagc tatagcctga cagttctgaa attgattcat taaggatagc ctctttcgtc
     3961 aacctgttag cccaaagcgt gttgacgcaa ataaatgcgc cgctctggct ttgagttaat
     4021 tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg
     4081 ccaggcgttg cacaaccggt catcacaccc gtattcgggc agtgtagcgt taattcgcga
     4141 ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc
     4201 tgtagagggg gagaaaattc cattgcgaca atccttgtta ttgcgttagc gcccagcgtt
     4261 ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg gtgaactccg
     4321 tcaccgcata cgaccagtta aaacctggcg gcgggattgt caccgcctcg ctggcgtcgc
     4381 gcaataaggt aatatgtgga tgaaacggac gattgctttg aaaacaaccg ctgcgggcag
     4441 cctgtgaacg gagcatattc gccagctgga ttaagccgcg tggaggctga cgcatcccta
     4501 accacaccac acgcgaacgc agccattgtc cggcgtcatc aagcgtgagt gtgaaaccag
     4561 gttgacgaat ccgtccggct aaaagagaaa gcgccttctc tttctcggcg ctcacttcgc
     4621 ctaaaaatgc cagagtcaga tgcaaattat cggcagcgac cggacgtcca gcctcaagtg
     4681 ggaagtgtgt ggcgcgccag tggataatct gttcgcggat ttctgcaggt aagtcgatag
     4741 caaagaacag acgttgcggg tcagacatgt gaggcactcg gttatgaatt accgcgatgc
     4801 tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt tgtgtcgttg ttgcccgttg
     4861 ctgccgtctt acctgaatta cttaccgctc tcgattgtgc gccgcaggtg ttattaagtg
     4921 cgccgaccgg ggccgggaaa tcaacctggc tgccgttgca actgctggcg catcccggca
     4981 ttaacgggaa aattatcctg ctggagccgc gtcgtctggc ggcgcgtaac gtcgcgcaac
     5041 ggctggcgga actgcttaac gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc
     5101 aaaactgcgt cgggccgaat acccgcctgg aagtggttac cgaaggcgtg ctgacgcgga
     5161 tgatccagcg tgacccggaa ctgagcggtg ttggactggt gatccttgat gaatttcatg
     5221 agcgcagctt gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc
     5281 gtgatgacct taaactgctg attatgtcgg ctacgctgga taacgaccgc ttgcagcaaa
     5341 tgctgccaga agcgcccgtc gtcatctcag aagggctctc gtttccggtt gaacgccgtt
     5401 atttaccgct gcccgcgcat cagcgttttg acgaagccgt cgcggttgcc accgccgaaa
     5461 tgctgcgtca ggaaagtgga tcattactgt tatttttacc tggcgtcgga gaaattcagc
     5521 gtgtgcagga acaactggct tcgcgcatcg gcagtgatgt gttgctctgc ccgctgtatg
     5581 gcgcgttgtc gctgaacgat cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca
     5641 aagtggtgct ggcgaccaat attgctgaaa ccagtttaac catcgaaggt attcgtctgg
     5701 tggtggattg tgcccaggaa agggtggcgc gttttgatcc gcgaacaggg cttacgcggt
     5761 tgattactca gcgaatcagt caggcgtcga tgacgcagcg tgccgggcgc gccgggcgtc
     5821 tggagccggg tatctgcctg catttaatcg ccaaggaaca agcagaacgc gccgcggcgc
     5881 aaagtgaacc ggaaatatta caaagcgatc tttccggttt gttgatggaa ttactgcaat
     5941 ggggatgcag cgatccggcg cagatgagct ggctggatca accgccagca gtgaatttac
     6001 tggccgcgaa acgcctgtta aagatgctgg gggcgctgga cggtgaacgg cttagtgcgc
     6061 aggggcaaaa aatggcagcg ctgggtaacg atccgcgttt agcggcaatg ctggttagcg
     6121 caaagagcga cgacgaagct gctaccgcgg caaaaattgc cgccattctc gaagagccgc
     6181 cacggatggg caatagtgac ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc
     6241 aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg cggtgaggca gacagttcgc
     6301 ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat tgctcgccgc cgtgggcaag
     6361 atggacgcta tcaactggca aacggcatgg gagcgatgct tgatgccgac gacgcgctaa
     6421 gtcgccacga atggttgatc gcgccgttat tattgcaggg cagcgcctcg ccggatgcgc
     6481 ggattttact ggcgttaccg gtcgatattg atgaattagt acaacgctgt ccgcagctgg
     6541 tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg tactctgaaa gcctggcgtc
     6601 ggctgcaaat cggtcagttg acggtaaaag tgcagccgct ggcgaaaccc tcagaagacg
     6661 agtttcatca ggcgatgctt aatggcatcc gtgataaagg tttaagcgtg ctcaactgga
     6721 cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc cgcaaagtgg ttgccggaat
     6781 atgactggcc agcggttgat gatgaaagtt tgttggcgac gctggaaacg tggctgttgc
     6841 cacatatgac cggcgtacat tcactacgcg gcctgaaatc actcgacatt tatcaggcac
     6901 tacgcggatt acttgattgg ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt
     6961 acactgtgcc gacgggaagc cggatcgcca ttcgttatca tgaagataac ccgcccgcgc
     7021 tggcggtgag aatgcaggag atgtttggtg aggccactaa tccgacgatc gcccaggggc
     7081 gcgtgccgct ggtgctggaa ttgctttcac ctgcccaaag gccgctgcaa atcacgcgtg
     7141 atttgggggc cttctggaaa ggggcgtatc gtgaggtgca aaaagagatg aaagggcgtt
     7201 atcccaaaca cgtctggccg gacgacccgg caaacactgc accgacgcga cggacgaaaa
     7261 agtattcgta aggttggttt tctccctctc cctcaaggga gaggggaccg atcgagcaca
     7321 aattttgaga gatatcttct tctgtcttgt aacagaagaa cagaaaatcg ggcttttgcg
     7381 cctgaatatt gcggagaaaa agcatggccg ggaatgaccg cgagccaatt ggacgcaaag
     7441 ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg tcgttacgaa gatgacgatg
     7501 attacgacga ttatgatgac tatgaggatg aagaaccgat gccgcgcaaa ggtaagggca
     7561 aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct atggctgctg ttaaaactgg
     7621 ctatcgtttt tgccgtgctg atcgccattt acggcgttta tctcgatcaa aaaattcgca
     7681 accgtattga tggcaaggtc tggcaactgc ctgcggcagt ttatggccga atggtcaacc
     7741 ttgagccaga catgaccatc agcaagaacg aaatggtgaa gctgctggag gcaacccagt
     7801 atcgtcaggt gtcaaaaatg acccgtcctg gcgaatttac cgtgcaggcc aacagcattg
     7861 agatgattcg ccgtccgttt gatttcccgg acagtaaaga aggacaggtg cgcgcgcgtc
     7921 tgacctttga tggcgatcat ctggcgacga tcgtcaatat ggagaacaac cgtcagttcg
     7981 gtttcttccg tcttgatccg cgtctgatca ccatgatctc ctcaccaaac ggtgagcagc
     8041 gtttgtttgt gccgcgcagt ggtttcccgg atttgctggt ggatactttg ctggcgacag
     8101 aagaccgcca tttctacgag catgatggaa tcagtctcta ctccatcgga cgtgcggtgc
     8161 tggcaaacct gaccgcaggt cgcacggtgc agggggcgag tacgctgacg caacagctgg
     8221 tgaaaaacct gttcctctcc agcgagcgct cttattggcg taaagcgaat gaagcctaca
     8281 tggcgctgat catggacgcg cgttacagca aagaccgtat tcttgagctg tatatgaacg
     8341 aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg cttcccgctg gcgagcttgt
     8401 attactttgg tcgcccggta gaagaattga gcctcgatca gcaggcgctg ttagtcggta
     8461 tggtgaaagg agcgtccatc tacaacccgt ggcgtaaccc gaaactggcg ctggagcgac
     8521 gtaatctggt actgcgtctg ctgcaacagc aacagattat tgatcaagaa ctctatgaca
     8581 tgttgagtgc gcgtccgctg ggtgttcagc cgcgcggtgg ggtgatctct cctcagccag
     8641 cctttatgca actggtgcgt caggagcttc aggctaaatt aggcgataag gtaaaagatc
     8701 tctccggcgt gaagatcttc actacctttg actcggtggc ccaggacgcg gcagaaaaag
     8761 ccgccgtgga aggcattccg gcacttaaga aacagcgtaa gctgagcgat ctggaaaccg
     8821 cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc gatggtcgga ggttctgagc
     8881 cgcagtttgc gggctacaac cgtgcgatgc aggcgcggcg ttcgattggt tcccttgcaa
     8941 aaccagcgac ttatctgacg gctttaagcc agccgaaaat ctatcgtctg aatacgtgga
     9001 ttgcggatgc gccaatcgcg ctgcgtcagc cgaatggcca ggtctggtca ccgcagaacg
     9061 atgaccgtcg ttatagcgaa agcggcagag tgatgctggt ggatgcgttg acccgttcga
     9121 tgaacgtgcc gacggtaaat ctggggatgg cgctggggct gcctgcggtt acggagacct
     9181 ggatcaaact gggtgtaccg aaagatcagt tgcatccggt tccggcaatg ctgctggggg
     9241 cgttgaactt aacgccaatc gaagtggcgc aggcattcca gactatcgcc agcggtggta
     9301 accgtgcacc gctttctgcg ctgcgttcgg taattgcgga agatggcaag gtgctatatc
     9361 agagcttccc gcaggcggaa cgcgctgttc cggcgcaggc ggcgtatctg acactatgga
     9421 ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct tggggcgaaa tacccgaacc
     9481 tgcatctggc agggaaaaca gggactacca acaataacgt agatacatgg tttgcgggca
     9541 ttgacggcag cacggtgacc atcacctggg tcggccgtga taacaaccag ccgaccaaac
     9601 tgtatggtgc cagcggggca atgtcgattt atcagcgtta tctggctaac cagacgccaa
     9661 cgccgctgaa tcttgttccg ccagaagata ttgcagatat gggcgtggac tacgacggca
     9721 actttgtttg cagcggtggc atgcgtgtct tgccggtctg gaccagcgat ccgcaatcgc
     9781 tgtgccagca gagtgagatg cagcagcagc cgtcaggcaa tccgtttgat cagtcttctc
     9841 agccgcagca acagccgcaa cagcaacctg ctcagcaaga gcagaaagac agcgacggtg
     9901 tagccggttg gattaaagat atgtttggta gtaattaacg tctaaacgtg acataccgga
     9961 tggcgagttg ccatccggta aaataacatc ccacctaaag tattaaccct ttcttttcat
    10021 ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg aacccgccag
    10081 cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat cattcacttt
    10141 acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac tcactgcgta
    10201 aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca caggcagcgg
    10261 ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa gaaagcgcat
    10321 gggggcctgc tgcaactatt gcggcgcgac agtcagctac cggcactaaa accgatacgc
    10381 cgattcaaaa agtgccacag tctatttctg ttgtgaccgc cgaagagatg gcgctgcatc
    10441 agccgaagtc ggtaaaagaa gcgcttagct acactcctgg cgttgccgtg ggaactcgtg
    10501 gcgcatccaa cacttacgat tacctgatca tccgcggatt cgccgctgac ggccaaagcc
    10561 agaacaacta tctgaatggc ctgaagatgc agggcaactt ctataacgat gcggttattg
    10621 acccatatat gctggagcgc gctgaaatca tgcgtggccc ggtttccgtg ctgtatggga
    10681 aaagcagccc aggtggcctg ttgaacatgg tcagcaagcg cccgaccact gaaccgttga
    10741 aagaagttca gtttaaggcc gggactgaca gcctgttcca gaccggtttt gacttcagcg
    10801 atgcgctgga tgatgacggc gtttactctt atcgtctgac cggtcttgcg cgctctgcca
    10861 atgcccagca aaagggcgcg gaagagcagc gttatgccat tgcgccagcg tttacctggc
    10921 gtccggacga taaaaccaat ttcaccttcc tttcttactt ccagaacgag ccggaaaccg
    10981 gttattacgg ctggttgccg aaagagggaa ccgttgagcc gctgccgaac ggtaagcgtc
    11041 tgccgacaga ctttaacgaa ggggcgaaga acaacaccta ttctcgtaat gagaagatga
    11101 ttggctatag cttcgaccac gaatttaacg acacctttac cgtacgtcag aaccttcgct
    11161 ttgcgcaaaa taaagtttcg caaaagagcg tatatggcta cggcatgtgt tcagatcctc
    11221 tctattcctc aaacccctct tcgagtccat gtgccaacgt gccgcagtct caatggggcc
    11281 atacactgac tcgtcagtac gttatcgata atgagaagtt agaaaacttc tctgttgata
    11341 ctcagttgca aagcaaattc gcaaccggtt cagtggatca cactctgctg actggcgttg
    11401 actttatgcg tatgcgtaat gacatcgact cctggttcgg atatgccggt tcagttgcac
    11461 catctgatat ctataattta gaccgtagtg actttgattt tggcgctcac ccaaatccgt
    11521 ctggtccata ccgcgttctg ctgaaacaga aacaaaccgg tctgtatgtt caggatcagg
    11581 cgcagtggga taaagtgctg gttacgcttg gcggtcgcta tgactgggcc gatcagtcat
    11641 ctttcaaccg tgattacggt aataaatccg atcgcgatga taaacagttc acctggcgtg
    11701 gcggtgttaa ctatctgttc gacaacggcg taacgcctta tttcagctac agtgaatcgt
    11761 ttgaacccgc ttcgcagacc gatgcaaacg gcgatctgtt tgcaccttcg aaaggcaaac
    11821 agtatgaagt cggcgtgaaa tatgtgccag aagatcgccc aattgtggtg actggcgcac
    11881 tgtatcagct taccaaaact aataacctga tggcggatcc gaacggttca ttgttctcgg
    11941 ttgaaggcgg tgagattcgt gcgcgtggcg tagagctgga agcgaaagcg gcactatcgg
    12001 cgagtgttaa cttagttggt tcttacacct acaccgatgc ggaatacacc accgatacta
    12061 cctataaagg caatacgccg gcacaggtgc caaaacacat ggcttcgctg tgggctgact
    12121 ataccttctt tgacggtccg ctttcaggtc tgacgctggg caccggtggt cgttatactg
    12181 gctccagcta tggtgatccg gctaactcct ttaaagtggg aagttatacg gtcgtggatg
    12241 cgttagtgcg ttatgatctg gcgcgagtcg gcatggctgg ctccaacgtg gcgctgcatg
    12301 ttaacaacct gttcgatcgt gaatacgtcg ccagctgctt taacacttat ggctgcttct
    12361 ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt ctcttttggg
    12421 gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca cgaatcattc
    12481 cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgta cgcttttgca
    12541 tccgctgtcg ttaacctttc cagccgggaa agtgaccggt ctgattggtc acaacggttc
    12601 tggtaaatcc actctgctca aaatgcttgg ccgtcatcag ccgccgtcgg aaggggagat
    12661 tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgttcgccc gcaaagtggc
    12721 ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac tggtggcgat
    12781 tggtcgttac ccatggcatg gcgctctggg gcgctttggt gcggcagatc gcgaaaaagt
    12841 tgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg tcgatagtct
    12901 ctctggcggt gagcgtcagc gggcgtggat cgccatgctg gtggcgcagg atagccgttg
    12961 tctgttgctc gacgaaccga cctcggcgtt ggatatcgcc caccaggttg atgtcctggc
    13021 gctggtgcac cgtttaagtc aggaacgtgg tctgacggtc attgccgtgc tgcacgatat
    13081 caatatggcg gcacgctact gtgattatct ggtcgccttg cgcggcggtg aaatgattgc
    13141 tcagggaacg cctgcggaaa ttatgcgcgg cgataccctc gaaatgattt atggcatccc
    13201 gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt attgatgagc
    13261 ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc tccgttgtta
    13321 tggcagatga ataccgccca cgcggcggct attgatccca atcgtattgt ggcgctggag
    13381 tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttatggcgt ggcggacacc
    13441 atcaactatc gcctgtgggt tagcgaacca ctgttacctg aatcggtgat tgacgtcggt
    13501 ttgcgcacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt tatggtctgg
    13561 tcggcaggat atggcccttc accagaaatg ctggcacgca ttgcgccggg gcgcggattt
    13621 aacttcagcg atggcaaaca gccgctggcg atggcgcgta aatcgctgac ggaaatggcg
    13681 gatttactta acctgcaaag cgcagccgaa acgcatttag cgcaatacga agactttatc
    13741 cgcagcatga aaccccgctt tgtgaagcgt ggtgcgcgtc cgttattgct gacgacgctt
    13801 atcgatccgc gccatatgct ggtcttcggt ccaaacagct tgttccagga aattcttgat
    13861 gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag taccgccgtc
    13921 agcatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga tcacgacaac
    13981 agcaaagaca tggatgcgct aatggcaacg ccgctgtggc aggccatgcc gtttgtccgc
    14041 gccggacgct ttcagcgcgt acccgcagtc tggttttatg gtgcaacgct ctcggcaatg
    14101 cactttgtgc gtattctcga taacgccatt ggagggaaag cgtgagtaaa cgaattgcgc
    14161 tttttccggc gttattgctg gcgctgctgg tgattgtcgc aatggcgttg acctggatga
    14221 atttctctca ggcgctgccg cgtagccagt gggcgcaggc cacctggtcg ccggatattg
    14281 acgtcatcga gcagatgatt tttcactaca gcttgttgcc gcgtctggcg atttcgctgc
    14341 tggtgggcgc gggcctgggg ctggtgggcg tgctgtttca gcaagtgctg cgtaacccgc
    14401 tggcggaacc gacgacgctt ggcgttgcaa cgggcgcgca actggggatt accgtcacta
    14461 cgctctgggc gatcccaggt gcgatggcga gccagtttgc tgcgctggcg ggcgcttgcg
    14521 ttgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg gctttcgccg gtaacgctga
    14581 tcctcgcggg gctggtggtg agcctttatt gcggggcaat caatcagtta ctggttatct
    14641 tccatcatga ccaactgcaa agcatgttcc tgtggagcac cggaacgctg acgcaaaccg
    14701 actggggcgg cgtcgagcgt ttatggccgc agctgctggg cggcgtgatg ctgacgttgc
    14761 tgctacttcg cccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc
    14821 tggccttgtc gctggcgcgt ctggcggcgt tgtcgctggc gattgtcatc agtgcgctgc
    14881 tggtgaacgc agtggggatt atcggtttta tcgggttgtt cgcgccgctg ctggcaaaaa
    14941 tgctgggggc gcggcgactg ctgccacggc tgatgctggc gtcgctgatt ggggcgctga
    15001 tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg gaagtttcca
    15061 ccggttcggt gactgcgttg atcggtgcgc cgctgctact gtggctgttg ccgcgtttac
    15121 gcagcattag tgcgccggat atgaaggtca acgatcgtgt cgcgactgaa cgccaacatg
    15181 tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg gtggcgctgt
    15241 cgtttggtcg tgatgcgcac ggctggacgt gggcgagcgg ggcgttgctc gaggatttga
    15301 tgccctggcg ctggccgcga attatggcgg cgctgtttgc gggcgtcatg ctggcggtgg
    15361 cgggctgtat tattcagcgg ctgaccggaa acccgatggc aagcccggaa gtgctgggga
    15421 ttagctctgg cgcggcgttt ggcgtggtac tgatgctgtt tctggtaccg ggtaatgctt
    15481 ttggctggct gttacccgcg ggaagtatcg gtgccgcggt gacgctgttg attattatga
    15541 tcgccgccgg gcgcggtgga ttttccccac accgcatgtt actggcgggg atggcgttaa
    15601 gtaccgcatt caccatgctt ttaatgatgt tgcaggcgag tggtgacccg cgaatggcgc
    15661 aagtgctgac ctggatctct ggttcgacct acaacgccac cgatgcgcag gtctggcgca
    15721 ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtgccgccgc tggctgacca
    15781 ttttaccgct gggtggtgat accgcgcggg ccatgggggt ggcgttaacg ccgacgcgaa
    15841 ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacgatgacc attggaccgt
    15901 tgagttttgt tggtttaatg gcaccgcata tcgcgcggat gatgggcttt cgacggacga
    15961 tgccgcacat cgtgatttcg gcgctggtgg gtggtttact gctggtgttc gctgactggt
    16021 gtgggcggat ggtgctgttt ccgttccaga tcccggcggg gctgctgtcg acctttatcg
    16081 gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaataaaat gcctcgcccg
    16141 gtgggcgagg caaaatcatc acaacttcgc aaacacccga cgtgcagcat cgatggtgtt
    16201 attgatatct tccatgctgt gcgccacaga cataaagccc gcttcaaacg ctgacggtgc
    16261 caggtaaaca ccttcatcca gcatcatatg gaagaaacgc ttaaaacgct cgacgtcaca
    16321 ggccatcaca tcctgataac acgtcacgga cccggcgtcg gtaaagaaaa taccgaacat
    16381 gccgccaacg tggtttacca ccagcggaat tccggcttct tctgccgctt ccagcagacc
    16441 ttctgccaga cgtgttgtca gctcatccag cgtttcgtga acgcccggct gcgcgacttc
    16501 attcagacag gcgaaaccgg ctgccatcgc aatcgggtta ccggaaagcg tacccgcctg
    16561 atagaccgga cccgtcgggg ccagcgcatc cattacatca cgacgaccac cgaatgcgcc
    16621 taccggcatt ccaccgccga tgattttgcc gaggcaggtt aaatctggca ctacgccgta
    16681 ataatcctgt gcgccagcta gcgctacgcg gaaaccggtc atcacttcat cgatgatcag
    16741 caacgcgcca aattcgtcac acagcgcacg cagacctggc aggaactctg gcagcggcgg
    16801 aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct cttgcgggta
    16861 ttgctcaaac gcggcgcgta cagaagccag atcgttataa gtacaggtta aggtatgttt
    16921 ggcgaaatct gccggaacgc ccggcgagtt tggctggcct aacgtgagtg cgccagaacc
    16981 ggcttttacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt taataatttt
    17041 gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atggtcgcct cggtgccgga
    17101 gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt
    17161 cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt caatcacggc
    17221 attgcggatc gccggatggt tatggcccag caccatcggc ccccaggaac cgacataatc
    17281 gatataggct ttgccatcaa catcgtacag ataagcaccg tccgcttttt cgataaacag
    17341 tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc cagggatcag
    17401 ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct gattcgtaga
    17461 aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg tcaaacatca
    17521 ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt gcatttgaaa
    17581 cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc atcttatttg
    17641 tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa acaccgcagg
    17701 ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgggcgcgat aaaaccccgt
    17761 tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggct gcggttgctt
    17821 ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta catactgctg
    17881 ataattatcc gctactgtta accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg
    17941 gctacttttt ggtgcgcaaa tacgcgcccg aagcaggggg gtcggggatc ccggaaattg
    18001 aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtattgccg gtgaaattct
    18061 ttggcgggct ggggacactc ggcggaggca tggtgctagg gcgcgaagga ccaaccgtgc
    18121 agatcggcgg taatatcggg cggatggtac tcgatgtttt ccgcctgaaa ggtgatgaag
    18181 ctcgccatac gctgttggca accggtgcgg cggcgggtct ggcggcggcg tttaacgcgc
    18241 cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc tatacgttaa
    18301 tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac cggattttta
    18361 atcatgaagt tgcgttgatt gacgtcggta aactttctga cgcgccgctt aatacgctgt
    18421 ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt aataaatggg
    18481 tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc aaatgggtac
    18541 tgatgggtgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt
    18601 cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc atgggaatgc
    18661 tggtgtttat cttcgtcgcg cgggtcatta ccaccttact ctgcttctct tccggcgcgc
    18721 cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc gctttcggaa
    18781 tggttgccgt tgagctgttt ccgcaatatc accttgaggc ggggacgttt gctattgccg
    18841 gaatgggggc gttactggcg gcatctattc gcgcgccgtt aacggggatc attctggttc
    18901 tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt cttggcgcaa
    18961 cactattagc gcaatttacc ggcgggaaac cgctatactc ggcgattctt gcgcgcacgc
    19021 tggcgaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagct agcgagaata
    19081 cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag aatttgccca
    19141 attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg ctggagttta
    19201 ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac ccgaatctga
    19261 aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt ttcacctttg
    19321 atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc ctggtggttg
    19381 atccgatgag cctgcaatat ctggtcggcg gttccgttga ctataccgaa ggactggaag
    19441 gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct
    19501 ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct tctctcttca
    19561 acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa tagccgccag
    19621 ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg gtacggaaaa
    19681 tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg cgtagatttc
    19741 tgtacgtaaa atcatgggga tttcgcgggc cagaacatca cgaatgatcc cgccgccaac
    19801 gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat taaaggcttt
    19861 attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg gcaacatcca
    19921 ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctag ttaccattgc
    19981 aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca gcgccatgtc
    20041 gcgaattgtc ccgccgccta cagcggtaac cacgcccagt accagaacac caaaagggtc
    20101 catacgcaat tttccggcta acaaaacgcc ggagatggca aataccgctg tgccgactat
    20161 atccagccaa tagacgagca ttgttcaatc cccactgagc acctaaaaag gtcaggcgct
    20221 aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata cgtgggcttg
    20281 cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt tcaccccagt
    20341 attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc tgtggcgagc
    20401 gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct ttaaagatgt
    20461 tttctccgcc acaaacttcg agtacctggt tttgaatcga ctcttttcca ctggtaaatg
    20521 gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca tattgcgctt
    20581 tcaattgcgc gtactgatcc agcagggatt gcgcggcttg ttcggcctta tccggttgcg
    20641 gactccaggg gaccagttga cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga
    20701 cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg gcattacctc
    20761 cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga ttcatcccct
    20821 gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa tagctgctga
    20881 ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga gaaagcgtga
    20941 tgacgcgcgg tgccgcgttg agccacagtg gcgcgagaaa agacagggcg accagcgccc
    21001 tgaacagtga cttagccatg tgccagtttc tgcaccagtg actcaaccat caggctggac
    21061 tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg atcggccacg
    21121 tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac atgggcgatt
    21181 gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat tttcgccaga
    21241 ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac agcgttaaga
    21301 ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct
    21361 gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatc cgcgtcgtga
    21421 taacgtgctt cgtccgagac aacgatatcg cccactttca acgttggtgc caggccaccg
    21481 gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcaa agtggcaccc
    21541 agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt tccattcagt
    21601 tggccggtat agatttcgca accgccgaga ctgatagttt gacggttttc gattttgtca
    21661 cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat agatttactc
    21721 gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc gtatttctca
    21781 cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc atcgtcgtta
    21841 ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg agagcgatcg
    21901 cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc
    21961 actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag tccagcaggt
    22021 ggggcgctac atcgccaaag aaattttaag ccgtctgaaa gagcttaaat tgctggaagc
    22081 atacggcctg gatgaactga ccggaccttt tgaaagcatt gttgagatgt catgcctgat
    22141 gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcggcgataa atgactggtt
    22201 tcgccagcgt ttgcacccgg aagatgccga aagccagcct ctgactgacg atcgctgcag
    22261 cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc ggcgcaagat
    22321 tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt
    22381 gatgaggatg aatctcacct gggcacaggt tggcggtatt ttaaaatata cccgtccggc
    22441 gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc cgggttatta
    22501 tctttctgaa gaagcctata ttgcccggtt gcgtaaagaa cttaatttgg cgctttacag
    22561 tcgttttcca ttaacgtgga ttatggaagc tgccgacgac atctcctatt gtgtggcaga
    22621 ccttgaagat gcggtagaga aaagaatatt taccgttgag cagctttatc atcatttgca
    22681 cgaggcgtgg ggtcagcatg agaaaggttc gctcttttcg ctagtggttg aaaatgcctg
    22741 ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt ttatgtattt
    22801 acgggtaaac accctaaata aactggtacc ctacgcggca caacgattta ttgataatct
    22861 gcctacgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca gcgaatgcag
    22921 cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc atccagatgt
    22981 cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga tttatcgtcc
    23041 tttattaagc ctgtcgttat cagactttac tgaactggta gaaaaagaac gggtgaaacg
    23101 tttccctatt gaaacgcgct tattccacaa actctcgacg cgccatcggc tggcctatgt
    23161 cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg aatattatta
    23221 ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg cgtgggatga
    23281 ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg aacaataaat
    23341 ttttaccttt tgcagaaact ttagttcgga acttcgcgct ataaaacgaa tctgaagaac
    23401 acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa ccacattagc
    23461 actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg caacggcggc
    23521 tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga tgctcgaaaa
    23581 ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta atacgccgcg
    23641 tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc aggaaggttc
    23701 tccgttccag agctctccgt tctgccaggg tggccagggc ggtaatggtg gcggccagca
    23761 acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag gctatgtcgt
    23821 caccaacaac cacgttgttg ataacgcgac ggtcattaaa gttcaactga gcgatggccg
    23881 taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc tgatccaaat
    23941 ccagaacccg aaaaacctga ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg
    24001 tgattacacc gtagcgattg gtaacccgtt tggtttgggc gagacggtaa cttccgggat
    24061 tgtctctgcg ctggggcgta gcggcctgaa tgccgaaaac tacgaaaact tcatccagac
    24121 cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg gttaacctga acggcgaact
    24181 gatcggtatc aacaccgcga tcctcgcacc ggacggtggc aacatcggta tcggttttgc
    24241 aatcccgagc aacatggtga aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa
    24301 acgcggtgag ctgggtatta tggggactga gctgaactcc gaactggcga aagcgatgaa
    24361 agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa
    24421 agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccga tcagcagctt
    24481 tgccgcactg cgtgctcagg tgggtactat gccggtgggc agcaaactga ccctgggctt
    24541 actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg cagcagagca gccagaatca
    24601 ggttgattcc agctccatct tcaacggcat tgaaggcgct gagatgagca acaaaggcaa
    24661 agatcagggc gtggtagtga acaacgtgaa aacgggcact ccggctgcgc agatcggcct
    24721 gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca gtgaaaaaca tcgctgaact
    24781 gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc gcggcgacag
    24841 caccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaaaac gggaaggggt
    24901 tctccttaca atctgtgaac ttcaccacaa ctccatacat cttcatcatc ctttaggcat
    24961 ttgcacaatg ccgtacgtta cgtactttct tatgctaagc cgtgcataac ggaggactta
    25021 tggctggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt accatgcgca
    25081 tcatcgatac caatatcaac gtaatggatg cccgtgggcg aattatcggc agcggcgatc
    25141 gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag ggacgagtcg
    25201 tcgatatcga tgacgcggtg gcacgtcatc tgcacggtgt gcggcaaggg attaatctac
    25261 cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc
    25321 tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt
    25381 tgatgcactt gttggcgcag gatagccgtt tgcgggagga actggtgatg aacctgattc
    25441 aggcagagga gaatactccc gcacttactg aatgggcgca acggctgggg atcgatctca
    25501 atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggt gtggacagcg
    25561 caatggcgga gttacaacaa ctgcaaaacg cgctgactac gcccgagcgt aataatctgg
    25621 tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac tcttttgggc
    25681 gctgggatgc agaagatcat cgtaagcgag ttgaacaact gattacccgc atgaaagagt
    25741 acggccagct gcgttttcgc gtttcactgg gcaactattt taccggtcct ggcagtattg
    25801 cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg atgccagaaa
    25861 gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt ttgcgtggcg
    25921 actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg gacaataacg
    25981 gcttgctgcg acgaacgctg gcggcgtggt ttcgtcacaa tgtgcaaccg ctggcaacgt
    26041 caaaggcgtt gtttattcat cgtaataccc tggagtatcg gcttaatcgt atatcggaac
    26101 tgaccgggct tgatttgggc aattttgatg acaggttgct gctgtatgta gcgttacaac
    26161 tggatgaaga gcggtaggtt atgcgttaag gttgaggagg cgctggctca ttcccctcag
    26221 cccgaagggg agaggggaat gcccgagtta accgtcaatt cgggattatt tacgcgttag
    26281 tttttccaga tcggcttcaa tctcgctgat cttattggtt acgactgact ccaggtgacg
    26341 taagtcgtcg aggatcttac gcttaagatc aacttcgctg cggtcacgct gacagatttg
    26401 atcaagctca tcaatgatat accgtagatt cggactgatt tcctggactt ctttataacc
    26461 ctgacccaca ccatcagcga cgaccgtttt acgctgacgc ggatatttaa acttaacgct
    26521 cttggcgaaa aactcgcctt tgtctttctg gaaatagatt ttcagaatat cgttgttggc
    26581 ttcctgccgg aggctgtaac gatcaatttc ttcaggattg gtaataccca gacttttcag
    26641 attgtcgtac atagcgttac ctcaaaatga gtcagtaaat tgtgcttatt ttagcatttg
    26701 gcctggcccg cccggctcga ctgtgatcgc agaaagcctt tttcttccag acttctatca
    26761 tggcgtaaaa atcaaaaaat tacctgcttt attgtggcga tattccatgg gatggattag
    26821 cacatttatc ttcgttttgc gaattacatg acagtccgaa acaagatgaa aaagtattta
    26881 aaaaaggggg aggaagaatg agtaggacga attattctga accagaaagc aatcgggcag
    26941 cgtcgtgctg cccgtgtgca tacttttagt cgatggtacg cagcagttcg ttaatgccga
    27001 ctttgccgcg agttttcgcg tcaactttct taacgataac cgcacagtag aggctgtatt
    27061 tgccatcttt cgacggcaga ttacctgaaa caaccacaga ccccgccgga acgcgaccgt
    27121 agtggatttc gccggtttca cggtcgtaaa tacgggtgct ctgaccaatg tatacgccca
    27181 tggaaatgac ggaaccttct tcgacaatca ccccttcaac cacttcagag cgcgcgccga
    27241 tgaagcaatt atcttcaatg atggttgggt tagcctgcag cggttccagc acaccgccga
    27301 tgcccacgcc accggaaagg tggacgtttt taccaatctg cgcacaagaa ccgacggtcg
    27361 cccaggtatc aaccatggtg ccttcatcaa catatgcgcc gatgttgacg taagatggca
    27421 tcagcacggt gttacgggca ataaacgcac cctgacgtac cgccgcaggt ggtacaacgc
    27481 ggaagccttc tttctggaaa cgtgcttcgt cgtagtcggc gaatttcatc ggcactttgt
    27541 cgaagtagcg gctttctgcc ccttcgatca cctgattatc attaatacgg aaagagagca
    27601 gcaccgcttt tttcaaccac tgatgcgtca cccactgacc gtcaattttt tccgctacac
    27661 gcagtgcgcc ggaatccagc agggcgatca cctgatttac cgcttcgcgg gtaacggtgt
    27721 ctgcattggc tggcgtgatc tcggcacggc gttcaaaagc ggtttcaata atgttctgta
    27781 actgctgcat tgttaaactc ttttcatatc agtaaacaca tcacccttta tcgtttggat
    27841 tgagggcctc tgtcaaccgc tgatgcactt cctgctgcaa ctcgttatta agtgcacgcc
    27901 ggtcagcggt ggcaattatg aataaatctt ctactcgctc gccaatggtt gtaattcggg
    27961 caccatgaag cgaaattccc agatcggcaa aaattttccc gactcgcgcc agcagcccag
    28021 gttggtcgag ggcgatcagt tcgaggaacg atttgcggtc ggtatgggtt ggcaaaaacg
    28081 tcacttcggt ttcaacagta aaatggcgca atttggcggg ttggcgacgg ggctgcggtg
    28141 gctgccagct actttgtgtc agtacttgct ccagaccaaa ccgaataacc tcatggcgat
    28201 ctgcggacag cgggctgcca tcgggttcca gcacgatgaa ggtatccatc gccataccgt
    28261 cgcgagtggt gaaaatttgt gcgtcgtgga cacttaaatt gcggcggtct aattcggcac
    28321 agacggcggc aaacagatag gggcggtccg ggctccagat aaaaatctcg gtgcctccac
    28381 gcgtagcctg cggactaagc aataccagcg gtttgcttaa atcatgctgt aataaatggc
    28441 gcgcatgcca ggccagttga tttgggctat ggcggacaaa atagttagca cgacagcgtg
    28501 accaaatttg gtgcagcgcc tcttcgtcga tgttatccat gcgcagcagt gccagcgcct
    28561 ggagttggtg atggcgaacc cgttcgcgca tatccggcgt gttttgcatt ccgcgtcgta
    28621 gctgtttttc ggtggcaaaa tagagttcgc gcaacagact ttgcttccag ctattccaca
    28681 gcgtttcgtt ggtggcacaa atgtcagcca ctgtcaggca taccagatag cgcagacgat
    28741 tttccgtttg aacttcttcg gcaaactgct tgatgacttc cgggtcctga atatcgcgac
    28801 gctgagctgt caccgacatc agcaagtgct ggcgaaccag ccaggcgacc agctgtgttt
    28861 cgcgtgagtt cagcccgtgg agttcggcaa aatgcactac atcctgagca ccgagaatag
    28921 agtggtcgcc gccgcgtcct ttggcgatat cgtgaaacag cgcggcgatg aaaatcagct
    28981 cagttgacgg taggcgcggc cagacgtcca cacacaacgg atggcgctgg cgcgtttctt
    29041 cactggcaaa actctccagt ttcagcatca cgcggatagt atgttcatcc accgtgtagg
    29101 cgtggaacag atcaaactgc atctgcccga cgatatgtga ccattgcggc atatacgcgc
    29161 cgagtacgct atggcgatgc attggcaata gcccgcgccg caccgctccg gggtgacgca
    29221 gaatgctcaa aaacagtttt cgtgcttccg gaatattaca cagcggttgt tgcagatggc
    29281 gccgggcatg gcgtaactgg cgcaacgtgg tggagtaaat gccggtgatc gcactgttgc
    29341 gcaccatggt gtagaacata cgcaagatgg cttccggctg gcgcataaat agtgtttcat
    29401 cacgcaggtc gattagcgta ccgcgtagct gaaactcatc gtcgattgga cgtggttttt
    29461 cgtcggcggg aagggcgagg atggcttcat cgaacagttg cagcagcatc tggttgagtt
    29521 cactgacgcg gcgtgtcacg cggaagtaat ccttcatcat ccgctcgacc ggttcgttac
    29581 cttcgccact gtaattcaga cgctgggcga cgctaagctg gcgatcgaat aacaggcgat
    29641 tatcgtaacg gctgacgacc agatgcaggg caaagcgaat acgccacaat atatgcagac
    29701 attcgtttaa ttccgcccgc tccgctgagg ttaagaagcc aaacccaacc atttcatcca
    29761 gcgatgttgc gccaaaatga cggcgggcca cccattgcag agtgtggata tcgcgcaagc
    29821 cgccagggct acttttgatg tctggttcaa gattgtagct ggtgccgtgg taacgctgat
    29881 ggcgctggtt ctgttcttca actttcgccg cgtagaactt gtcggaaggc cagaatcctt
    29941 cgctgaaaat atgtttttgc agttcaagaa atagcgcaac atcgccaatt aataagcggg
    30001 attcgatcaa attggtggcg acggttaaat ccgataaccc ttccagcatg cactcttcaa
    30061 gcgtgcgcac actatgaccg acttccagct ttacatccca gagcagcgtt aacagctcgc
    30121 ccactttttg cgcctgatcg tccgggagct ttttacggct taaaatcagt aaatcaatgt
    30181 cagaaagtgg atgcagctcg ccacgaccgt agccaccgac ggcgaccaat gccaggtcgg
    30241 caatctggct gaatcccgct tcaatccata atcgttgcag gagctggtcg ataaactcgg
    30301 tgcgcgcctc aatcaactgt tctgcagaga tcccattgtc aaaggcatca cccagccaac
    30361 gctggaaagt atcgatatgg gcttttatcc caccgacggt taattcatca cggggccaga
    30421 cgcatggatt ttgcggttga ccgggcaggg tggggagagc ggtgtttgcg tactgttctg
    30481 gaagggtatt cattgtgcgc cacccataag attaaattat cgcattaaaa aagccggcat
    30541 tcgccggctt cattttattc gtcgtgcgag attatcgccg ggatggtgtc atccttgcgt
    30601 agtgtcagaa tttcgcagcc gttatcagtc accacaatag tatgctcata ttgtgcagac
    30661 aagctgcgat ctttggtttt taccgtccag ccatctttca tggtgcggat ctctttttta
    30721 cccgcgttga ccattggctc gatggtgaac gtcatcccag gtttcagtac gacgttggtt
    30781 tcacgggagt catagtgcag cacctgcggt tcttcatgga agccgcgacc aataccgtgt
    30841 ccgcaatatt cacgaacgac ggagaagcct tctgcttcga caaatttctg aatcgccgca
    30901 ccgatttcgc gcagattaat gcctggtttt accatgcgta gcgccaggta caggctttct
    30961 tgcgtgatac ggcacagacg ttcgcccatg atggtcggct taccgacgat aaacattttc
    31021 gaggtatcgc cgtggaaacc atctttgatt acggtgacat caatgttaac gatatcgcca
    31081 tctttcagca gcttagcatc gtccgggata ccgtggcaca ccacttcatt aattgagatg
    31141 caaacggatt tcggataacc gtgatagccg aggcaggcag aaaccgcgtg ttgttcatta
    31201 acaatgtaat cattacagat gcgatccagc tcgccggtgc tgacgcccgg tttaacatat
    31261 ggttcgatca tctccagcac ttcggcagcc agtcggccag cgacgcgcat tttttcgata
    31321 tcttctgggg tcttgattga gatagccatt aattctgtcc atcagcgtcg gtgataccga
    31381 caatatatat gtaagtgccg tcaatggtat cacacccggg caaattgaga atcattctga
    31441 atttcgccaa acgtgccact gaaggttttc tataatagaa aattcaacgt ctgatgctgt
    31501 acacagcgcc aacaattatt ggtgtccacg gcgtatttgt ggtataaagc gcgccggact
    31561 tccgatccat ttcgtataca cagactggac ggaagcgaca atctcacttt gtgtaacaac
    31621 acacacgtat cggcacatat tccggggtgc cctttggggt cggtaatatg ggatacgtgg
    31681 aggcataacc ccaactttta tatagaggtt ttaatcatgg caactgtttc catgcgcgac
    31741 atgctcaagg ctggtgttca cttcggtcac cagacccgtt actggaatcc gaaaatgaag
    31801 ccgttcatct tcggtgcgcg taacaaagtt cacatcatca accttgagaa aactgtaccg
    31861 atgttcaacg aagctctggc tgaactgaac aagattgctt ctcgcaaagg taaaatcctt
    31921 ttcgttggta ctaaacgcgc tgcaagcgaa gcggtgaaag acgctgctct gagctgcgac
    31981 cagttcttcg tgaaccatcg ctggctgggc ggtatgctga ctaactggaa aaccgttcgt
    32041 cagtccatca aacgtctgaa agacctggaa actcagtctc aggacggtac tttcgacaag
    32101 ctgaccaaga aagaagcgct gatgcgcact cgtgagctgg agaaactgga aaacagcctg
    32161 ggcggtatca aagacatggg cggtctgccg gacgctctgt ttgtaatcga tgctgaccac
    32221 gaacacattg ctatcaaaga agcaaacaac ctgggtattc cggtatttgc tatcgttgat
    32281 accaactctg atccggacgg tgttgacttc gttatcccgg gtaacgacga cgcaatccgt
    32341 gctgtgaccc tgtacctggg cgctgttgct gcaaccgtac gtgaaggccg ttctcaggat
    32401 ctggcttccc aggcggaaga aagcttcgta gaagctgagt aataaggcac gctcgtacga
    32461 gcccttatag accaggtagt acacgtttgg ttagggggcc tgcatatggc cccctttttc
    32521 acttttatat ctgtgcggtt taatgccggg cagatcacat ctccgaggat tttagaatgg
    32581 ctgaaattac cgcatccctg gtaaaagagc tgcgtgagcg tactggcgca ggcatgatgg
    32641 attgcaaaaa agcactgact gaagctaacg gcgacatcga gctggcaatc gaaaacatgc
    32701 gtaagtccgg tgctattaaa gcagcgaaaa aagcaggcaa cgttgctgct gacggcgtga
    32761 tcaaaaccaa aatcgacggc aactacggca tcattctgga agttaactgc cagactgact
    32821 tcgttgcaaa agacgctggt ttccaggcgt tcgcagacaa agttctggac gcagctgttg
    32881 ctggcaaaat cactgacgtt gaagttctga aagcacagtt cgaagaagaa cgtgttgcgc
    32941 tggtagcgaa aattggtgaa aacatcaaca ttcgccgcgt tgctgcgctg gaaggcgacg
    33001 ttctgggttc ttatcagcac ggtgcgcgta tcggcgttct ggttgctgct aaaggcgctg
    33061 acgaagagct ggttaaacac atcgctatgc acgttgctgc aagcaagcca gaattcatca
    33121 aaccggaaga cgtatccgct gaagtggtag agaaagaata ccaggtacag ctggatatcg
    33181 cgatgcagtc tggtaagccg aaagaaatcg cagagaaaat ggttgaaggc cgcatgaaga
    33241 aattcaccgg cgaagtttct ctgaccggtc agccgttcgt tatggaacca agcaaaactg
    33301 ttggtcagct gctgaaagag cataacgctg aagtgactgg cttcatccgt ttcgaagtgg
    33361 gtgaaggcat cgagaaagtt gagactgact ttgcagcaga agttgctgcg atgtccaagc
    33421 agtcttaatt atcgaaaagg agccgcctga gggcggcttc tttttgtgcc catcttgtaa
    33481 attcagctaa cccttgtggg gctgcgctga aaagcgacgt acaatgtcgc tagtattaat
    33541 tcatttcaat cgttgacagt ctcaggaaag aaacatggct accaatgcaa aacccgtcta
    33601 taaacgcatt ctgcttaagt tgagtggcga agctctgcag ggcactgaag gcttcggtat
    33661 tgatgcaagc atactggatc gtatggctca ggaaatcaaa gaactggttg aactgggtat
    33721 tcaggttggt gtggtgattg gtgggggtaa cctgttccgt ggcgctggtc tggcgaaagc
    33781 gggtatgaac cgcgttgtgg gcgaccacat ggggatgctg gcgaccgtaa tgaacggcct
    33841 ggcaatgcgt gatgcactgc accgcgccta tgtgaacgct cgtctgatgt ccgctattcc
    33901 attgaatggc gtgtgcgaca gctacagctg ggcagaagct atcagcctgt tgcgcaacaa
    33961 ccgtgtggtg atcctctccg ccggtacagg taacccgttc tttaccaccg actcagcagc
    34021 ttgcctgcgt ggtatcgaaa ttgaagccga tgtggtgctg aaagcaacca aagttgacgg
    34081 cgtgtttacc gctgatccgg cgaaagatcc aaccgcaacc atgtacgagc aactgactta
    34141 cagcgaagtg ctggaaaaag agctgaaagt catggacctg gcggccttca cgctggctcg
    34201 tgaccataaa ttaccgattc gtgttttcaa catgaacaaa ccgggtgcgc tgcgccgtgt
    34261 ggtaatgggt gaaaaagaag ggactttaat cacggaataa ttcccgtgat ggataaataa
    34321 gggtaagatt ccgcataagt atcgcggggg cgtaagtctg gttataaggc gttattgttg
    34381 caggcagttt ggttacggcc agcgcgcagc aaccggagcg tacaaaagta cgtgaggatg
    34441 gcgagcactg ctcggggcca aaatggcaaa taaaatagcc taataatcca gacgattacc
    34501 cgtaatatgt ttaatcaggg ctatacttag cacacttcca ctgtgtgtga ctgtctggtc
    34561 tgactgagac aagttttcaa ggattcgtaa cgtgattagc gatatcagaa aagatgctga
    34621 agtacgcatg gacaaatgcg tagaagcgtt caaaacccaa atcagcaaaa tacgcacggg
    34681 tcgtgcttct cccagcctgc tggatggcat tgtcgtggaa tattacggca cgccgacgcc
    34741 gctgcgtcag ctggcaagcg taacggtaga agattcccgt actctgaaaa tcaacgtgtt
    34801 tgatcgttca atgtctccgg ccgttgaaaa agcgattatg gcgtccgatc ttggcctgaa
    34861 cccgaactct gcgggtagcg acatccgtgt tccgctgccg ccgctgacgg aagaacgtcg
    34921 taaagatctg accaaaatcg ttcgtggtga agcagaacaa gcgcgtgttg cagtacgtaa
    34981 cgtgcgtcgt gacgcgaacg acaaagtgaa agcactgttg aaagataaag agatcagcga
    35041 agacgacgat cgccgttctc aggacgatgt acagaaactg actgatgctg caatcaagaa
    35101 aattgaagcg gcgctggcag acaaagaagc agaactgatg cagttctgat ttcttgaacg
    35161 acaaaaacgc cgctcagtag atccttgcgg atcggctggc ggcgttttgc tttttattct
    35221 gtctcaactc tggatgtttc atgaagcaac tcaccattct gggctcgacc ggctcgattg
    35281 gttgcagcac gctggacgtg gtgcgccata atcccgaaca cttccgcgta gttgcgctgg
    35341 tggcaggcaa aaatgtcact cgcatggtag aacagtgcct ggaattctct ccccgctatg
    35401 ccgtaatgga cgatgaagcg agtgcgaaac ttcttaaaac gatgctacag caacagggta
    35461 gccgcaccga agtcttaagt gggcaacaag ccgcttgcga tatggcagcg cttgaggatg
    35521 ttgatcaggt gatggcagcc attgttggcg cagctgggct gttacctacg cttgctgcga
    35581 tccgcgcagg taaaaccatt ttgctggcca ataaagaatc actggttacc tgcggacgtc
    35641 tgtttatgga cgccgtaaag cagagcaaag cgcaattgtt accggtcgat agcgaacata
    35701 acgccatttt tcagagttta ccgcaaccta tccagcataa tctgggatac gctgaccttg
    35761 agcaaaatgg cgtggtgtcc attttactta ccgggtctgg tggccctttc cgtgagacgc
    35821 cattgcgcga tttggcaaca atgacgcccg atcaagcctg ccgtcatccg aactggtcga
    35881 tgggacgtaa aatttctgtc gattcggcta ccatgatgaa caaaggtctg gaatacattg
    35941 aagcgcgttg gctgtttaac gccagcgcca gccagatgga agtgctgatt cacccgcagt
    36001 cagtgattca ctcaatggtg cgctatcagg acggcagtgt tctggcgcag ctgggggaac
    36061 cggatatgcg tacgccaatt gcccacacca tggcatggcc gaatcgcgtg aactctggcg
    36121 tgaagccgct cgatttttgc aaactaagtg cgttgacatt tgccgcaccg gattatgatc
    36181 gttatccatg cctgaaactg gcgatggagg cgttcgaaca aggccaggca gcgacgacag
    36241 cattgaatgc cgcaaacgaa atcaccgttg ctgcttttct tgcgcaacaa atccgcttta
    36301 cggatatcgc tgcgttgaat ttatccgtac tggaaaaaat ggatatgcgc gaaccacaat
    36361 gtgtggacga tgtgttatct gttgatgcga acgcgcgtga agtcgccaga aaagaggtga
    36421 tgcgtctcgc aagctgagga taatccggct acagagagtc gcgctatttg ttagcgtagg
    36481 gcttcagtga tatagtctgc gccatctgat cgtaagtagt tggctttata aggtcaggta
    36541 tgccgtggtt ttacacggct tttttttgta taggcttcag tattcctgag taccgtaaac
    36601 cctgtcaggg aataaaaaac gcgtgatgtt gtctgctact caaccactta gcgaaaaatt
    36661 gccagcgcat ggctgccgtc atgttgcgat cattatggac ggcaatggtc gctgggcaaa
    36721 aaagcaaggg aagattcgtg cctttgggca taaagccggg gcaaaatccg tccgccgggc
    36781 tgtctctttt gcggccaaca acggtattga ggcgttaacg ctgtatgcct ttagtagtga
    36841 aaactggaac cgaccagcgc aggaagtcag tgcgttaatg gaactgtttg tgtgggcgct
    36901 cgatagcgaa gtaaaaagtc tgcaccgaca taacgtgcgt ctgcgtatta ttggcgatac
    36961 cagtcgcttt aactcgcgtt tgcaagaacg tattcgtaaa tctgaagcgc taacagccgg
    37021 gaataccggt ctgacgctga atattgcggc gaactacggt ggacgttggg atatagtcca
    37081 gggagtcagg caactggctg aaaaggtgca gcaaggaaac ctgcaaccag atcagataga
    37141 tgaagagatg ctaaaccagc atgtctgtat gcatgaactg gcccctgtag atttagtaat
    37201 taggactggg ggggagcatc gcattagtaa ctttttgctt tggcaaattg cctatgccga
    37261 actttacttt acagatgttc tctggcccga tttcgatgaa caagactttg aaggggcgtt
    37321 aaatgccttt gctaatcgag agcgtcgttt cggcggcacc gagcccggtg atgaaacagc
    37381 ctgatggggg tcgcttttgc tgaagtatcg cctgatatct gcttttgtgt taatacccgt
    37441 cgtcatcgcg gcgttgtttc tgttgccgcc ggtggggttc gccattgtaa cgctggtggt
    37501 ctgcatgctg gcagcgtggg aatggggaca gcttagcggt tttaccactc gttcgcagcg
    37561 agtatggttg gcggtgttat gcgggttatt gttggcgctg atgctttttc tgttgccgga
    37621 atatcaccga aatattcatc aaccgctggt tgaaatctca ctttgggctt cgctgggttg
    37681 gtggattgtc gcgctattgc tggtgctgtt ttacccaggt tccgcagcaa tctggcgtaa
    37741 ctctaaaaca ttgcgcctta tttttggcgt gctaaccatt gttcccttct tctggggcat
    37801 gctggcgtta cgggcctggc actatgacga gaatcattac agtggcgcaa tatggctgct
    37861 ctatgtcatg atcctggtat ggggcgctga ctccggcgca tatatgtttg gcaaattgtt
    37921 tggtaaacat aagctggcac cgaaggtttc tccgggtaaa acctggcaag gctttatcgg
    37981 tggactcgct actgcagcgg taatctcatg gggttatggc atgtgggcga atctcgacgt
    38041 cgctcccgtc accttactca tttgctctat tgtcgcagcg ttagcctcag tgctcggcga
    38101 tctgaccgag agtatgttta agcgtgaagc aggaattaag gacagcggtc atttaattcc
    38161 aggacacggt ggtattttag atcgtattga tagcctgacg gctgcggtac cggtctttgc
    38221 ttgcttgttg ttactggtat tcaggacgct ttaacggaag gtaatatgct gagttttctc
    38281 tgggatttgg cttcgttcat cgttgcactg ggtgtactta tcaccgtgca tgaatttggt
    38341 catttctggg ttgcccggcg ttgtggtgtt cgcgttgagc gtttctcaat agggtttggt
    38401 aaggcgctct ggcggcgaac tgataagctc ggcaccgaat atgttatcgc cctgatcccg
    38461 ttgggcggtt atgtcaaaat gctggatgag cgcgcagaac cggtcgttcc ggaactccgc
    38521 caccatgcct tcaataataa atctgtcggc caacgagcgg cgattattgc cgcaggtccg
    38581 gttgcaaact tcatttttgc tatctttgcc tactggctgg tttttattat tggtgtgcct
    38641 ggcgtacgtc cggtggttgg cgaaatagca gccaattcga tagctgcgga agcacaaatt
    38701 gcaccaggta cggaactaaa agccgtagat ggtatcgaaa cgcctgattg ggatgccgtg
    38761 cgtttgcagt tggtcgataa aattggcgat gaaagcacca ccattacagt agcgccattt
    38821 ggcagcgacc aacggcggga tgtaaagctc gatttacgtc actgggcgtt tgagcctgat
    38881 aaagaagatc cggtatcttc gctggggatt cgtcctcgtg ggccgcaaat tgaacctgta
    38941 ctggaaaatg tgcagccaaa ctcggcggca agcaaggcag gtttgcaagc aggcgacagg
    39001 atcgttaaag tcgatggtca gcccttaacg cagtgggtga cctttgtgat gcttgtccgg
    39061 gataacccgg gtaaatcctt agcgttagaa atcgaaaggc aggggagtcc cttgtctttg
    39121 acattaatcc cggagagtaa accgggtaat ggtaaagcga ttggttttgt cggtattgag
    39181 ccgaaagtca ttcctttgcc agatgagtat aaagttgtac gccagtatgg gccgttcaac
    39241 gccattgtcg aagccacgga caaaacgtgg cagctgatga agctgacggt cagtatgctg
    39301 ggaaaattga tcaccggtga tgtgaaactg aacaacctca gtgggccgat ctctatcgcc
    39361 aagggggctg ggatgacagc ggaactcggg gtagtttatt acctgccgtt tcttgcgctt
    39421 attagcgtga acttagggat aattaacctg tttccgttgc ccgtacttga cggggggcat
    39481 ctgctgttcc ttgcgatcga aaagatcaag ggcggaccgg tatccgagcg ggttcaagac
    39541 ttttgttatc gcattggctc gattctgctg gtgctgttaa tggggcttgc acttttcaat
    39601 gatttctctc ggttatgaga gttagttagg aagaacgcat aataacgatg gcgatgaaaa
    39661 agttgctcat agcgtcgctg ctgtttagca gcgccaccgt atacggtgct gaagggttcg
    39721 tagtgaaaga tattcatttc gaaggccttc agcgtgtcgc cgttggtgcg gccctcctca
    39781 gtatgccggt gcgcacaggc gacacggtta atgatgaaga tatcagtaat accattcgcg
    39841 ctctgtttgc taccggcaac tttgaggatg ttcgcgtcct tcgtgatggt gatacccttc
    39901 tggttcaggt aaaagaacgt ccgaccattg ccagcattac tttctccggt aacaaatcgg
    39961 tgaaagatga catgctgaag caaaacctcg aggcttctgg tgtgcgtgtg ggcgaatccc
    40021 tcgatcgcac caccattgcc gatatcgaga aaggtctgga agacttctac tacagcgtcg
    40081 gtaaatatag cgccagcgta aaagctgtcg tgaccccgct gccgcgcaac cgtgttgacc
    40141 taaaactggt gttccaggaa ggtgtgtcag ctgaaatcca gcaaattaac attgttggta
    40201 accatgcttt caccaccgac gaactgatct ctcatttcca actgcgtgac gaagtgccgt
    40261 ggtggaacgt ggtaggcgat cgtaaatacc agaaacagaa actggcgggc gaccttgaaa
    40321 ccctgcgcag ctactatctg gaccgcggtt atgcccgttt caacatcgac tctacccagg
    40381 tcagtctgac gccagataaa aaaggtattt acgtcacggt gaacatcacc gaaggcgatc
    40441 agtacaagct ttctggcgtt gaagtgagcg gcaaccttgc cgggcactcc gctgaaattg
    40501 agcagctgac taagatcgag ccgggcgagc tgtataacgg caccaaagtg accaagatgg
    40561 aagatgacat taaaaagctt ctcggtcgct atggttatgc ctatccgcgc gtacagtcga
    40621 tgcctgaaat taacgatgcc gacaaaaccg ttaaattacg tgtgaacgtt gatgcgggta
    40681 accgtttcta cgtgcgtaag atccgttttg aaggtaacga tacctcgaaa gatgccgtcc
    40741 tgcgtcgcga aatgcgtcag atggaaggtg catggctggg gagcgatctg gtcgatcagg
    40801 gtaaggagcg tctgaatcgt ctgggcttct ttgaaactgt cgatacagat acccaacgtg
    40861 ttccgggtag cccggaccag gttgatgtag tctacaaggt gaaagaacgt aacactggta
    40921 gcttcaactt cgggattggt tacggtactg aaagtggcgt gagcttccag gctggcgtgc
    40981 aacaggataa ctggttaggt acaggttatg ctgttggtat caacgggacc aaaaacgatt
    41041 accagaccta tgctgaactg tcggtaacca acccgtactt caccgtagat ggcgtaagcc
    41101 tcggtggtcg tctcttctat aatgacttcc aggcagatga cgccgacctg tccgactata
    41161 ccaacaagag ttatggtaca gacgtgacgt tgggcttccc gattaacgaa tataactcgc
    41221 tgcgtgcagg tctgggttat gtacataact ccctgtccaa catgcagcct caggttgcga
    41281 tgtggcgtta tctgtactct atgggtgaac atccgagcac ctctgatcag gataacagct
    41341 tcaaaacgga cgacttcacg ttcaactatg gttggaccta taacaagctt gaccgtggtt
    41401 acttcccgac agatggttca cgtgtcaacc tgaccggtaa agtgaccatt ccgggatcgg
    41461 ataacgaata ctacaaagtg acgttagaca cggcgactta tgtgccgatc gatgacgatc
    41521 acaaatgggt tgttctgggg cgtacccgct ggggttatgg tgatggttta ggcggcaaag
    41581 agatgccgtt ctacgagaac ttctatgccg gtggttccag caccgtgcgt ggcttccagt
    41641 ccaataccat tggtccgaaa gcagtttact tcccgcatca ggccagtaat tatgatccgg
    41701 actatgatta cgaatgtgcg actcaggacg gcgcgaaaga cctgtgtaaa tcggatgatg
    41761 ctgtaggcgg taacgccatg gcggttgcca gcctcgagtt catcaccccg acgccgttta
    41821 ttagcgataa gtatgctaac tcggttcgta cttccttctt ctgggatatg ggtaccgttt
    41881 gggatacaaa ctgggattcc agccaatatt ctggatatcc ggactatagt gatccaagca
    41941 atatccgtat gtctgcgggt atcgcattac aatggatgtc cccattgggg ccgttggtgt
    42001 tctcctacgc ccagccgttc aaaaagtacg atggagacaa ggcagaacag ttccagttta
    42061 acatcggtaa aacctggtaa gtgttctcca caaaggaatg tagtggtagt gtagcgatga
    42121 ctttaggcga tcaatataag atcgccgggc cacgcaaaga actgcaccct ccggtgcaaa
    42181 tgggatggta aggagtttat tgtgaaaaag tggttattag ctgcaggtct cggtttagca
    42241 ctggcaacat ctgctcaggc ggctgacaaa attgcaatcg tcaacatggg cagcctgttc
    42301 cagcaggtag cgcagaaaac cggtgtttct aacacgctgg aaaatgagtt caaaggccgt
    42361 gccagcgaac tgcagcgtat ggaaaccgat ctgcaggcta aaatgaaaaa gctgcagtcc
    42421 atgaaagcgg gcagcgatcg cactaagctg gaaaaagacg tgatggctca gcgccagact
    42481 tttgctcaga aagcgcaggc ttttgagcag gatcgcgcac gtcgttccaa cgaagaacgc
    42541 ggcaaactgg ttactcgtat ccagactgct gtgaaatccg ttgccaacag ccaggatatc
    42601 gatctggttg ttgatgcaaa cgccgttgct tacaacagca gcgatgtaaa agacatcact
    42661 gccgacgtac tgaaacaggt taaataagta atgccttcaa ttcgactggc tgatttagcg
    42721 cagcagttgg atgcagaact acacggtgat ggcgatatcg tcatcaccgg cgttgcgtcc
    42781 atgcaatctg cacaaacagg tcacattacg ttcatggtta acccaaaata ccgtgagcat
    42841 ttaggcttgt gccaggcgtc cgcggttgtc atgacccagg acgatcttcc tttcgcgaaa
    42901 agtgccgcgc tggtagtgaa gaatccctac ctgacttacg cgcgcatggc gcaaatttta
    42961 gataccacgc cgcagcccgc gcagaacatt gcacccagtg cggagatcga cgcgacggcg
    43021 aagctgggta acaacgtatc gattggcgct aacgcggtga ttgaatccgg cgttgaactg
    43081 ggcgataacg tgattatcgg tgccggttgc ttcgtaggta aaaacagcaa aatcggtgca
    43141 ggttcgcgtc tctgggcgaa cgtaaccatt taccatgaga tccagatcgg tcagaattgc
    43201 ctgatccagt ccggaacagt ggtaggcgca gacggctttg gttatgccaa cgatcgtggt
    43261 aactgggtga agatcccaca gattggtcgc gtaattattg gcgatcgcgt ggagatcggt
    43321 gcctgcacta ccatcgatcg cggcgcgctg gatgacactg ttattggcaa tggcgtgatc
    43381 attgataacc agtgccagat tgcacataac gtcgtgattg gcgacaatac ggcggttgcc
    43441 ggtggcgtca ttatggcggg cagcctgaaa attggtcgtt actgcatgat cggcggagcc
    43501 agcgtaatca acgggcatat ggaaatatgc gacaaagtga cggttacggg catgggtatg
    43561 gtgatgcgtc ccatcactga accaggcgtc tattcctcag gcattccgct gcaacccaac
    43621 aaagtctggc gcaaaaccgc tgcactggtg atgaacattg atgacatgag caagcgtctg
    43681 aaatcgcttg agcgcaaggt taatcaacaa gactaacgtt ccatcttttg ttcgccaaac
    43741 tttacggcct gtctcattct tacgattgcg gcaggccgtg ttattattgt cgtttcttat
    43801 attttgacag gaagagtatc ttgactacta acactcatac tctgcagatt gaagagattt
    43861 tagaacttct gccgcaccgt ttcccgttct tactggtgga tcgcgtgctg gattttgaag
    43921 aaggtcgttt tctgcgcgca gtaaaaaatg tctctgtcaa tgagccattc ttccagggcc
    43981 atttccctgg aaaaccgatt ttcccgggtg tgctgattct ggaagcaatg gcacaggcaa
    44041 caggtattct ggcgtttaaa agcgtaggaa aactggaacc gggtgagctg tactacttcg
    44101 ctggtattga cgaagcgcgc ttcaagcgcc cggtcgtgcc tggcgatcaa atgatcatgg
    44161 aagtcacttt cgaaaaaacg cgccgcggcc tgacccgttt taaaggggtt gctctggtcg
    44221 acggtaaagt agtttgcgaa gcaacgatga tgtgtgctcg tagccgggag gcctgatacg
    44281 tgattgataa atccgccttt gtgcatccaa ccgccattgt ggaagagggc gcgtcgattg
    44341 gcgcgaacgc acacattggt cctttttgta tcgttggacc ccatgtcgaa attggtgagg
    44401 gtaccgtact gaaatctcat gttgtcgtga atggtcatac taaaattggc cgcgataatg
    44461 agatttatca gttcgcctcc atcggcgaag ttaaccagga tctgaaatat gctggcgaac
    44521 cgacccgcgt ggaaatcggc gatcgtaacc gcattcgcga aagcgtcacc attcatcgtg
    44581 gcacagtcca gggcggtgga ttgacgaagg tgggcagcga caacttactg atgatcaacg
    44641 cgcacattgc gcacgattgt acggtaggta accgctgtat tctcgccaac aacgcaacgc
    44701 tggcgggtca cgtatcggtt gacgacttcg cgatcatcgg cggcatgacc gcagtccatc
    44761 agttctgcat cattggtgcg catgtgatgg ttggcggctg ctccggtgtg gcgcaggacg
    44821 tccctcctta tgtcattgcg cagggtaacc acgccacgcc gttcggtgtc aatatcgaag
    44881 ggctgaagcg ccgcggattc agccgtgagg cgattaccgc tatccgcaat gcgtataagc
    44941 taatttatcg tagcggtaaa acgctcgatg aagtgaaacc ggaaattgct gaactggcgg
    45001 aaacatatcc ggaagtgaaa gcctttaccg atttctttgc acgctcaacg cgcggtctga
    45061 ttcgttaatg actgaacagc gtccattaac gattgccctg gtcgccggag aaacctccgg
    45121 cgatatcctg ggggccggtt taatccgcgc tctgaaagaa cgtgtgccca acgcccgctt
    45181 tgttggtgtt gccgggccac gaatgcaggc tgaaggctgc gaagcctggt acgaaatgga
    45241 agaactggcg gtgatgggca ttgttgaagt gctcggtcgt ctgcgtcgct tactgcatat
    45301 tcgtgccgat ctgacaaagc gttttggcga actgaagcca gatgtttttg ttggtattga
    45361 tgcgcccgac ttcaatatta ctcttgaagg taacctcaaa aagcagggta tcaaaaccat
    45421 tcattacgtc agtccatccg tctgggcgtg gcgacagaaa cgcgttttca aaataggcag
    45481 agccaccgat ctggtgctcg catttctgcc tttcgaaaaa gcgttttatg acaaatacaa
    45541 tgtaccgtgc cgctttatcg gtcataccat ggctgatgcc atgccgttag atccagataa
    45601 aaatggtgcc cgtgaagtgc tggggatccc gcacgatact cactgtctgg cattgttgcc
    45661 gggaagtcgt ggcgcggaag ttgagatgct tagcgccgat ttcctgaaaa cggcccagct
    45721 tttgcgccag acgtaccctg atctggagat cgtggtgccg ctggtgaatg ccaaacgccg
    45781 cgagcagttt gaacgcatca aagctgaagt cgcgccagac ctttcagttc atttgctgga
    45841 tggaatgggc cgtgaggcga tggtcgccag cgatgcggca ctactggcgt cggggacggc
    45901 agccctggag tgtatgctgg cgaaatgccc gatggtggtg ggatatcgca tgaagccttt
    45961 taccttctgg ttggcgaagc ggctggtgaa aactgaatat gtctcgctgc caaatctgct
    46021 ggcgggcaga gagttagtca aagaattatt gcaggaagag tgtgagccgc aaaaactggc
    46081 tgcggcgctg ttaccgctgt tggcgaacgg gaaaaccagc cacgcgatgc acgatacctt
    46141 ccgtgaactg catcagcaga tccgctgcaa tgccgatgag caggcggcac aagccgttct
    46201 ggagttagca caatgatcga atttgtttat ccgcacacgc agctggttgc gggtgtggat
    46261 gaagtcggac gcgggccgtt agttggcgcg gtcatcaccg ctgcggtgat ccttgacccg
    46321 gcgcgcccga ttgccgggct gaatgattcc aaaaagctga gcgaaaaacg ccgtctggcg
    46381 ctctatgaag agatcaaaga gaaagcgttg agctggagtc tgggccgcgc ggaaccccac
    46441 gaaatcgacg agctgaacat tcttcatgcg accatgctgg cgatgcagcg tgccgtcgct
    46501 gggctgcata ttgcgccgga atatgtgttg attgatggta accgctgccc gaaattaccg
    46561 atgccttcga tggcagtggt gaaaggcgat agccgcgtac cggaaatcag tgccgcgtct
    46621 atcctggcga aagtgacgcg tgacgccgaa atggcggcgc tggatattgt tttcccgcaa
    46681 tatggttttg cccaacacaa agggtatcca accgcttttc atctggaaaa actggctgaa
    46741 cacggcgtga ccgaacacca tcggcgcagc tttgggcctg tcaaacgcgc actgggactt
    46801 gcgtcctgat tcttgtgtcg agattaagta aaccggaatc tgaagatgtc tgaaccacgt
    46861 ttcgtacacc tgcgggtgca cagcgattac tcgatgatcg atggcctggc caaaaccgca
    46921 ccgctggtaa aaaaggcggc ggcgttgggt atgccagcac tggcgatcac cgatttcacc
    46981 aatctttgcg gtctggtgaa gttctacgga gcgggacatg gcgcagggat taagcctatc
    47041 gtcggggcag attttaacgt ccagtgcgac ctgctgggtg atgagttaac ccacctgacg
    47101 gtgctggcgg cgaacaatac cggctatcag aatctgacgt tactgatctc aaaagcgtat
    47161 cagcgcgggt acggtgccgc cgggccgatc atcgatcgcg actggcttat cgaattaaac
    47221 gaagggttga tccttctttc cggcgggcgc atgggcgacg tcggacgcag tcttttgcgt
    47281 ggtaacagcg cgctggtaga tgagtgtgtc gcgttttatg aagaacactt cccggatcgc
    47341 tattttctcg agctgatccg caccggcagg ccggatgaag aaagctatct gcacgcggcg
    47401 gtggaactgg cggaagcgcg cggtttgccc gtcgtggcga ctaacgatgt gcgctttatc
    47461 gacagcagcg actttgacgc acacgaaatc cgcgtcgcga tccacgacgg ctttaccctc
    47521 gacgatccta aacgcccgcg taactattcg ccgcagcaat atatgcgtag cgaagaggag
    47581 atgtgcgagc tgtttgccga catccccgaa gcccttgcca acaccgttga gatcgccaaa
    47641 cgctgtaacg taaccgtgcg tcttggtgaa tacttcctgc cgcagttccc gaccggggac
    47701 atgagcaccg aagattatct ggtcaagcgt gcaaaagagg gcctggaaga gcgtctggcc
    47761 tttttattcc ctgatgagga agaacgtctt aagcgccgcc cggaatatga cgaacgtctg
    47821 gagactgaac ttcaggttat caaccagatg ggcttcccgg gctacttcct catcgttatg
    47881 gaatttatcc agtggtcgaa agataacggc gtaccggtag ggccgggccg tggttctggt
    47941 gcagggtcac tggtggccta cgcgctgaaa atcaccgacc tcgatccgct ggaatttgac
    48001 ctgctgttcg aacgtttcct taacccggaa cgtgtctcca tgcctgactt cgacgttgac
    48061 ttctgtatgg agaaacgcga tcaggttatc gagcacgtag cggacatgta cggtcgtgat
    48121 gcggtatcgc agattatcac cttcggtacg atggcggcga aagcggtgat ccgcgacgta
    48181 ggccgcgtgc tggggcatcc gtacggcttt gtcgatcgta tctcgaaact gatcccgccc
    48241 gatccgggga tgacgctggc gaaagcgttt gaagccgagc cgcagctgcc ggaaatctac
    48301 gaagcggatg aagaagttaa ggcgctgatc gacatggcgc gcaaactgga aggggtcacc
    48361 cgtaacgccg gtaagcacgc cggtggggtg gttatcgcgc cgaccaaaat taccgatttt
    48421 gcgccgcttt attgcgatga agagggcaaa catccggtca cccagtttga taaaagcgac
    48481 gttgaatacg ccggactggt gaagttcgac ttccttggtt tgcgtacgct caccatcatc
    48541 aactgggcgc tggagatgat caacaagcgg cgggcgaaga atggcgagcc gccgctggat
    48601 atcgccgcga tcccgctgga tgacaagaaa agcttcgaca tgctgcaacg ctcggaaacc
    48661 acggcggtat tccagcttga atcgcgcggc atgaaggacc tgatcaagcg tctgcaacct
    48721 gactgcttcg aagatatgat cgcactggtg gctctgttcc gccctggtcc gttgcagtca
    48781 gggatggtgg ataactttat cgaccgtaaa catggtcgcg aagagatctc ctatccggac
    48841 gtacagtggc agcatgaaag cctgaaaccg gtactggagc caacctacgg catcatcctg
    48901 tatcaggaac aggtcatgca gattgcgcag gtgctttctg gttataccct cggtggcgcg
    48961 gatatgctgc gtcgtgcgat gggtaagaaa aagccggaag agatggctaa gcagcgttct
    49021 gtatttgctg aaggtgcaga aaagaacgga atcaacgctg aactggcgat gaaaatcttc
    49081 gacctggtgg agaaattcgc tggttacgga tttaacaaat cgcactctgc ggcctatgct
    49141 ttggtgtcat atcaaacgtt atggctgaaa gcgcactatc cggcggagtt tatggcggcg
    49201 gtaatgaccg ccgatatgga caacaccgag aaggtggtgg gcctggtgga tgagtgctgg
    49261 cggatggggc tgaaaatcct gccaccagat ataaactccg gtctttacca tttccacgtc
    49321 aacgacgacg gcgaaatcgt gtatggtatt ggcgcgatca aaggggtagg tgaaggtccg
    49381 attgaggcga tcatcgaagc ccgtaataaa ggcggctact tccgcgaact gtttgatctc
    49441 tgcgcccgaa ccgacaccaa aaagttaaac cggcgggtgc tggaaaaact gatcatgtcc
    49501 ggggcgtttg accgtcttgg gccacaccgc gcggcgctga tgaactcgct gggtgatgcg
    49561 ttaaaagcgg cagatcaaca cgcgaaagcg gaagctatcg gtcaggccga tatgttcggc
    49621 gtgctggccg aagaaccgga acaaattgaa caatcctacg ccagctgcca accgtggccg
    49681 gagcaggtgg tattagatgg ggaacgtgaa acgttaggcc tgtacctgac cggacaccct
    49741 atcaaccagt atttaaaaga gattgagcgt tatgtcggag gcgtaaggct gaaagacatg
    49801 cacccgacag aacgtggtaa agtcatcacg gctgcggggc tcgttgttgc cgcgcgggtt
    49861 atggtcacca agcgcggcaa tcgtatcggt atctgcacgc tggatgaccg ttccgggcgg
    49921 ctggaagtga tgttgtttac tgacgccctg gataaatacc agcaattgct ggaaaaagac
    49981 cgcatactta tcgtcagcgg acaggtcagc tttgatgact tcagcggtgg gcttaaaatg
    50041 accgctcgcg aagtgatgga tattgacgaa gcccgggaaa aatatgctcg cgggcttgct
    50101 atctcgctga cggacaggca aattgatgac cagcttttaa accgactccg tcagtctctg
    50161 gaaccccacc gctctgggac aattccagta catctctact atcagagggc ggatgcacgc
    50221 gcgcggttgc gttttggcgc gacgtggcgt gtctctccga gcgatcgttt attaaacgat
    50281 ctccgtggcc tcattggttc ggagcaggtg gaactggagt ttgactaata caggaatact
    50341 atgagtctga atttccttga ttttgaacag ccgattgcag agctggaagc gaaaatcgat
    50401 tctctgactg cggttagccg tcaggatgag aaactggata ttaacatcga tgaagaagtg
    50461 catcgtctgc gtgaaaaaag cgtagaactg acgcgtaaaa tcttcgccga tctcggtgca
    50521 tggcagattg cgcaactggc acgccatcca cagcgtcctt ataccctgga ttacgttcgc
    50581 ctggcatttg atgaatttga cgaactggct ggcgaccgcg cgtatgcaga cgataaagct
    50641 atcgtcggtg gtatcgcccg tctcgatggt cgtccggtga tgatcattgg tcatcaaaaa
    50701 ggtcgtgaaa ccaaagaaaa aattcgccgt aactttggta tgccagcacc agaaggttac
    50761 cgcaaagctc tgcgcctgat gcaaatggct gaacgcttta agatgccaat catcaccttt
    50821 atcgacaccc cgggggctta tccgggcgtg ggcgcagaag aacgtggtca gtctgaagcc
    50881 attgcacgca acctgcgtga aatgtctcgc ctcggtgtac cggtagtttg tacggttatc
    50941 ggtgaaggtg gttctggcgg cgcgctggcg attggcgtgg gcgataaagt gaatatgctg
    51001 caatacagca cctattccgt tatctcgccg gaaggttgtg cgtccattct gtggaagagc
    51061 gccgacaaag cgccgctggc ggctgaagcg atgggtatca tcgctccgcg tctgaaagag
    51121 ctgaaactga tcgactccat catcccggaa ccgctgggcg gcgctcaccg taacccggaa
    51181 gcgatggcgg catcgttgaa agcgcaactg ctggcggatc tggccgatct cgacgtgtta
    51241 agcactgaag atttaaaaaa tcgtcgttat cagcgcctga tgagctacgg ttacgcgtaa
    51301 ttcgcaaaag ttctgaaaaa gggtcacttc ggtggccctt ttttatcgcc acggtttgag
    51361 caggctatga ttaaggaagg attttccagg aggaacacat gaacatcatt gccattatgg
    51421 gaccgcatgg cgtcttttat aaagatgagc ccatcaaaga actggagtcg gcgctggtgg
    51481 cgcaaggctt tcagattatc tggccacaaa acagcgttga tttgctgaaa tttatcgaac
    51541 ataacccgcg gatctgcggc gtgatttttg actgggatga atacagtctc gatttatgta
    51601 gcgatatcaa tcagcttaat gaatatctcc cgctttatgc cttcatcaac acccactcga
    51661 cgatggatgt cagcgtgcag gatatgcgga tggcgctctg gttttttgaa tatgcgctgg
    51721 ggcaggcgga agatatcgcc attcgtatgc gtcagtacac cgacgaatat cttgataaca
    51781 ttacaccgcc gttcacgaaa gccttgttta cctacgtcaa agagcggaag tacacctttt
    51841 gtacgccggg gcatatgggc ggcaccgcat atcaaaaaag cccggttggc tgtctgtttt
    51901 atgatttttt cggcgggaat actcttaagg ctgatgtctc tatttcggtc accgagcttg
    51961 gttcgttgct cgaccacacc gggccacacc tggaagcgga agaatacatc gcgcggactt
    52021 ttggcgcgga acagagttat atcgttacca acggaacatc gacgtcgaac aaaattgtgg
    52081 gtatgtacgc cgcgccatcc ggcagtacgc tgttgatcga ccgcaactgc cataaatcac
    52141 tggcgcatct gttgatgatg aacgatgtag tgccagtctg gctgaaaccg acgcgtaatg
    52201 cgttggggat tctggggggg atcccgcgcc gtgaatttac tcgcgacagc atcgaagaga
    52261 aagtcgctgc caccacgcag gcacaatggc cggttcatgc ggtgatcacc aactccacct
    52321 atgatggctt gctctacaac accgactgga tcaaacagac gctggatgtc ccctcgatcc
    52381 acttcgattc tgcctgggtg ccgtacaccc attttcatcc gatctaccag ggtaaaagtg
    52441 gtatgagcgg tgagcgtgtt gcgggaaaag tgatcttcga aacgcaatcg acccacaaaa
    52501 tgctggcggc gttatcgcag gcttcgctga tccacattaa aggtgagtat gacgaagagg
    52561 cgtttaacga agcctttatg atgcatacca ccacctcgcc cagttatccc attgttgctt
    52621 cggttgagac ggcggcggcg atgctgcgtg gtaatccggg caaacggctg attaaccgtt
    52681 cggtggagcg ggcgctgcat tttcgcaaag aggtccagcg gttgcgggaa gagtctgacg
    52741 gttggttttt cgatatctgg caaccgccgc aggtggatga agccgaatgc tggcccgttg
    52801 cgcctggcga acagtggcac ggctttaacg atgcggatgc cgatcatatg ttcctcgatc
    52861 cggttaaagt cactattctg acgccgggga tggacgagca gggcaatatg agcgaggagg
    52921 ggatcccggc ggcgctggtg gcgaagttcc tcgatgagcg tgggatcgtg gtagagaaaa
    52981 ccggcccata taacctgctg tttctcttta gtattggcat cgataaaacc aaagcaatgg
    53041 gattattgcg tgggttgacg gaatttaaac gctcttacga tctcaatctg cgaatcaaaa
    53101 atatgcttcc ggatctgtac gcagaagatc ccgatttcta ccgcaatatg cgtattcagg
    53161 atctggcgca agggatccat aagctaattc gtaaacacga tcttcccggt ttgatgttgc
    53221 gggcattcga tactttgccg gaaatgatca tgacgccaca tcaggcatgg cagagacaga
    53281 ttaaaggtga agtagaaacc attgcgctgg aacaactggt cggtagagta tcggcaaata
    53341 tgatcctgcc ttatcctcct ggcgtaccgc tgttgatgcc tggagaaatg ctgaccaaag
    53401 agagccgcac ggtactcgat tttctactga tgctttgttc cgtagggcaa cattaccccg
    53461 gttttgaaac ggatattcac ggcgcgaaac aggacgaaga cggcgtttac cgcgtacgag
    53521 tcctaaaaat ggcgggataa cttgccagag cggcttccgg gcgagtaacg tgctgttaac
    53581 aaataaagga gacgttatgc tgggtttaaa acaggttcac catattgcga ttattgcgac
    53641 ggattatgcg gtgagcaaag ctttctactg cgatattctt ggtttcacgc tgcaaagcga
    53701 agtctatcgc gaagcgcgcg actcgtggaa aggggatttg gcgcttaatg ggcaatatgt
    53761 gattgagctt ttctcatttc cgttcccgcc ggagcgcccc agccgaccgg aagcttgcgg
    53821 cctgcgtcat ctggctttta gcgttgatga catcgatgcg gcagtggcgc accttgaaag
    53881 ccataacgtg aagtgtgaag ccatccgtgt cgatccatac acgcaaaaac gcttcacctt
    53941 ctttaacgat ccggacgggc tgccgttgga actgtatgag cagtaaggct tgtcatcgcc
    54001 gcatttgccc ggtaacgtgc cgggcattgc tactgtaaaa tcgcaccatc atgacactca
    54061 cgctcaatag acaacttctc acctcacgcc agattctggt ggcctttagc ggcgggcttg
    54121 actccaccgt tctgctgcat cagttggtgc agtggcggac ggaaaatccg ggtgtcgctc
    54181 tgcgcgctat tcatgtgcat cacggtttaa gtgccaatgc cgatgcctgg gttacgcatt
    54241 gcgaaaacgt ctgccaacag tggcaggtgc cgctggtggt cgaacgcgta caacttgcgc
    54301 aagaggggct ggggattgag gcccaggcgc ggcaggcgcg ttatcaggca tttgcccgca
    54361 ccttgttgcc cggtgaagtg ctggtcaccg cgcaacatct cgacgatcaa tgtgaaactt
    54421 ttctgctggc gctaaaacgc ggtagcggcc ctgccgggct ttcggctatg gcggaagttt
    54481 cggagtttgc cggaacacgg cttatccgcc cgttgcttgc ccgcacgcgg ggggaactgg
    54541 cgcagtgggc attggcgcat ggtttacgct ggattgaaga cgaaagtaat caggacgact
    54601 catacgatcg taactttctg cgcatgcgcg tagtgccgtt attgcagcag cgttggccgc
    54661 attttgccga aacaacggcc cgcagcgccg cactttgtgc tgaacaagag agcctgctgg
    54721 atgaactgct ggcagatgat ttagcacact gtcaatcgcc gcaggggacg ctgcagattg
    54781 tgccaatgct ggcgatgagt gatgcccgcc gcgcggcgat tatccgccgc tggctggcag
    54841 ggcagaatgc accgatgcct tcccgcgacg cgttggtgag gatctggcag gaagtggcgc
    54901 tggcgcggga agatgcctcc ccctgtttac gtttgggcgc gtttgaaatc cgacgctatc
    54961 agtcgcaact gtggtggatt aaatccgtca ccgggcaaag cgaaaacatt gtgccgtggc
    55021 agacgtggct tcaaccgctg gaattaccgg cggggctggg aagtgtacag cttaatgcgg
    55081 gaggtgctat tcgccctccg cgtgcagacg aagcggtcag cgtgcgtttc aaagcgccag
    55141 gattgctgca tattgtcggg cgtaacggcg gacgtaagct aaagaaaatc tggcaagagc
    55201 tgggcgtgcc gccgtggcta cgtgacacca cgccactgct gttttatggc gaaacgctga
    55261 ttgcggcggc aggggtattt gtgacgcaag aaggtgtggc tgaaggtgag aatggcataa
    55321 gttttgtctg gcagcgaaac gcttagttaa gtgaaggccg gatatgacgc attaagcgtc
    55381 gcatccggcg aaagtcaatc aggactcgct caccaccacc gtaccgattt ccgggtggct
    55441 aaagctggta attttatcca gacgtagctc acgggtttcg ccagcggcct cgacgaccag
    55501 atactccaca tttttgcggg agactaaatc gctggctttt gcctgcaatt tttcgccatc
    55561 tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc
    55621 atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc agtaagtttg
    55681 ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt
    55741 cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg
    55801 cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca
    55861 gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa
    55921 ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt attccgttac
    55981 aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc
    56041 gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc
    56101 gcgccaccct tcagaaaccc aggagcgtat gatgctgcgc ttgctggcgt ggctgaaata
    56161 tgctgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg
    56221 gctgcgtaac gatcacctgg gcattgattt gtggattgag ctggggctgc cggatgagcg
    56281 gcggattaag aaagcctgca cccaggccgc agaagtggcg ctgtttacct ataatagtcg
    56341 ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gtgcagtttg ccaatctttc
    56401 cgtctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg atcgtaccat
    56461 gacgctgcag gcaacgattc aggatggcgt gatctggtta tcggatgata agaataatct
    56521 ggaagtgaac ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg acatgttgct
    56581 attcccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat
    56641 gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc cagcctgcca
    56701 gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag tgatggcgtg
    56761 attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc agctctggcc
    56821 cggctggtgg ctgtgattaa agaattaaca acagaacaaa aagcccgacg acccacgcgg
    56881 cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag
    56941 gcgatgcgcg gcaaagtgcg cagcggtcgg gaataaaaag aaggaatgga tggtgaaaaa
    57001 agcgatagtg acagcgatgg ctgtaatcaa cctctttact ctgatgggtt gtaataatcg
    57061 ggccgaagtc gatacgcttt ctccggcgca ggcagccgaa ctgaaaccga tgccgcaaag
    57121 ttggcgcggc gtgctgccgt gtgccgattg cgaaggaatc gaaacctctc tgttcctcga
    57181 aaaagacgga acatgggtga tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc
    57241 cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa
    57301 aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg
    57361 taaaccgatt gaatcgcagt tcaactatac gctggaagcg gcacaatcca gtttacccat
    57421 gacgccgatg actctgcggg gcatgtattt ttatatggct gatgcggcga ccttcactga
    57481 ttgcgcgacc ggaaaacgtt tcatggtagc gaataacgca gagctggagc gtggctacct
    57541 ggctgcgcgc ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc actttacgct
    57601 tgaggctaat ccggataccg gtgcgccgac taaagtattg gcacccgata cggcagggaa
    57661 attttaccca aaccaggatt gcagtagttt ggggcagtaa acctgcttaa ggcagaaaca
    57721 aacgcaaaac aggccagaag gatatatttc aacattttga atttgcacgt ttttttgtag
    57781 gccggataag gcgtttacgc cgcatccggc aatggtgctc aacgcctgat gcgacgctgg
    57841 cgcgtcttat catgcctaca atccccctca taccttaagg cggataaggc atttacttta
    57901 tcaccgggtc aaaccaacaa agcgacccgc cttgatataa atccctggct tcaggtgccc
    57961 aacatactgt aactgcgttt cgggcttaaa cgcagagaca tcgccagtac gcatatgcat
    58021 cagatcggca gggctaatcc agcccgattg cgccagcgta agcggatgcc cggctttggc
    58081 aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt ttgtctcctt cgcccacact
    58141 gcttaactgc gctttcgcca ggccgctgac gcactgttgg cgaaaatcct cggagaacgg
    58201 attcagtgag cacatctggc gagtcaccat aaagggaata aattcgacaa tgccgcgata
    58261 gttataaccg ctatctttga ttttattggc gaacgcggtg atttctgtgg cttgttgcgg
    58321 ggtaagatcc gggactcgta agacgaaaag tttatcacta tgcttcatcg cttttttaag
    58381 ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg ttattttcac ccagatagat
    58441 tgcaacgtga ctcacagagg aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag
    58501 gcttgaggag aacagcaaat cgccgggttt gagatcaggt accgttattt cttttattga
    58561 ttgttcggtg aaagagcttt gatgctggaa tttgacagcc catgtttttg cctcgacatc
    58621 taccgctgtg gcagaggagt ctggctggct gatatcaacg gtacaggccg aaagtaaaag
    58681 aaagctgggg agaagcaggc ggtagtacgc ctttggttta cccattttat acaatccatg
    58741 taaaaaaggg ccctgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca
    58801 gatattcgac gatgtcacca gtcttaatta actgtttctc gccgttgcga cgatatttat
    58861 attcgatatc gtcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga
    58921 tcagttccat atcagcaaac atcacgcccg gacgctcttt gcggtcatcc agcagcactt
    58981 cgataccttg tgcgcgcagt tcgctgtaca gtttctcagc aagctcttgt acgcggaagg
    59041 atttgtgcat gttcatcggc agaatcgcca cctggaacgg cgcgatagcg tcaggccata
    59101 cgatgccgcg ttcgtcgtag ttctgctcaa tcgccgcagc tactacacgc gttaccccga
    59161 taccgtagca acccatagtc aggatttggt tacggccatc ttcaccctgt acggaggctt
    59221 tcagtgcttc ggagtacttg gttcccagct ggaagatgtg accaacttcg ataccacgtt
    59281 tgatcagcag cgtaccctga ccatccgggc ttggatcgcc cgccaccacg ttgcggatat
    59341 cagcaatttc cggggtagcg acatcgcgat cccagttgat gccgaagtag tgtttaccat
    59401 cgatgttagc accagcagcg aaatcactca tcgccgcaac ggtacggtca atcaccaccg
    59461 gaatcggcat gtttaccgga cccagtgaac ccggaccggc tttaaccacg gcacgaattt
    59521 cttcttcggt cgcgaaagtc agcgggctgg caacctgcgg cagtttttct gctttaactt
    59581 cgttcagctc gtgatcaccg cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa
    59641 ccgctttaac aagcagagtc ttaaccgttt tcttaatcgg cagattgaac tgttcaacca
    59701 gttccgcgat ggttttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag
    59761 cgcgcggttc tttcggcgcg atagcttctg ccagttcaat gttcgctgca tagtcagagg
    59821 tgtcggagaa gaccacatcg tcttcaccgc tctgcgccag cacctggaat tcgtgagagg
    59881 cgctgccgcc gatagaaccg gtgtcggcct gtacggcgcg gaaatccagc cccatgcggc
    59941 tgaagatttt gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag
    60001 aagtatggaa agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac
    60061 gcggacgcac ttcgtcgcgg aacttggtct ggatctgata gaagttcagc ggcagctgtt
    60121 tgtaagagct aagctcgtta cgaatcaggt cagtgataac ttcttcatga gttgggccga
    60181 gtacgaacgg acgctcgcca cggtcaacaa aacgcagcag ttccggaccg tactgttccc
    60241 aacgaccact ctcttgccac aaatcggctg gctgaaccac cggcatcgac acctcgattg
    60301 caccggcgtt gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc
    60361 cggtcggcag ccaggtatat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca
    60421 tcagctgatg gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt
    60481 attggctagt acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggcgag
    60541 cctgggacaa aaaaagtgat ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc
    60601 gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct gcgtcggtga tgcgccagcg
    60661 aacgttaaaa tcatgcagcc agacggcata ggttttgccc gtttcctcac ctttacgata
    60721 ggccgggcgc gggtcttgcg ccagtacttc gcggataaac agggttaact gcggataacg
    60781 cttctccagc gtcaaaagct gcttttcgac ctctgcggta aaactcaccg tcatctctgc
    60841 cgctggcgca ctttgcgcat agctggcaca ggcatcggga agcgattcgg caaaggggag
    60901 atacggtttg atatccacta ccggcgtacc atcgaccaga tccagactgc cgagcttcag
    60961 aatcacgctg tctttatggc aaacaacctc tttcagctct accagcgaca taccaattgg
    61021 gttagggcga aaagtggaac gcgtagcgaa aacacccatt ctggcattac cgccaagacg
    61081 aggcggacgc acagtcggac gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat
    61141 ccataaatgg ctgaatgctt ccaggccgcg aacggcgtcg gcctggttgt agggagcaat
    61201 gagatgcagt tcgccgttgg cgctttttac cagaccaggc tggcgcggaa cggcgaattt
    61261 ttctttatag ggcgagcgaa taacgcctat ttgctcgaac tggaaactgc tcatttcgcc
    61321 gtaatgttaa gcgcagaacc gatacataca gcctgacgat agcagcctgg cgtaccgctg
    61381 gtgacttcgc agctatgcag taataccgca ttggctttca ttttcgaggc gttgatttgc
    61441 atccgcttac gcgcggttgg aatgctcggc ggagagtcct gattagaggc ctggcaagag
    61501 tcgccactga cttcaccgag atcgcggaac ggtttgccga ctaattcttc tgcattggta
    61561 taaattcgga ccggcgtggc gcgcggcgct ttcggttttg caggctccgc tttcggctgg
    61621 ggtgcagtgc tttgaacggg ttcgacgggg gatctgctta acatggaaca gccgcttagc
    61681 atgagtgcta ctaaacagat cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa
    61741 acgtcaatat tgaatcagga gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa
    61801 tgatattaca aattgtggaa acagcctaaa aattaccagc ctttaacagc gccgccgtta
    61861 aacactttgt ttgctgcttc gtaaacttcg tcagactgat aagcctggac gaatttcttc
    61921 acgttctcgg cgtctttgtt atcttcacgc gttacgatca ggtttacgta cggggagtct
    61981 ttatcttcga caaagatacc gtctttcgct ggagtcaggc caatctggct ggcataggtg
    62041 gtgttaataa ctgccagagc gatttgcgcg tcgtccagag aacgcggcag ttgcggtgct
    62101 tccagctcaa caattttcag atttttgggg ttctcaacaa catcaagaac ggtcggcagc
    62161 aggccaacgc catctttcag tttgatcaag cccacttttt gcagcagcag cagtgaacga
    62221 ccaaggttag ttgggtcgtt tggcacggca acctgcgaac catcctgcag ttcatccagt
    62281 gatttgattt tcttggagta accagcaatc ggataaacaa aagtgttgcc tactgcgacc
    62341 agtttgtagc cacgatcttt cagttgctga tcaaggtacg gtttatgctg gaaggcgttg
    62401 gcgtcgatat cgcctttgct caatgcttcg tttggcagaa catagtcgtt gaaggttacc
    62461 agctcaacgt ccaggccata tttgtctttc gcaactttct gcgcgacttc tgcaacctgc
    62521 tgttcggcac caacaatcac gccgacttta atgtggtttg gatctttttc atcctgaccg
    62581 cagcctacca gtgccagtga tccaatcagg gctcccactg ccgcaaaggt tttgaatttg
    62641 aacgccatac cttattcctt cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg
    62701 acagcccgga cgatgcggtc gcctgcgaac tgaattaaat aaaccagaat gaccagcaat
    62761 accagtaccg tattcatcac cgtcgcgttg tagccgatgt agccatactg atagccaatc
    62821 tgacctaaac caccggcacc gactgcacca cccatcgcgg aataaccgac cagggtaatc
    62881 agggtgatag ttgccgcatt caccagaccc ggcaacgctt ccggtaacag cactttacgg
    62941 acgatctgca ttggcgtggc acccattgcg cgggaagctt caattaaccc ggttgggatc
    63001 tccagcagag cgttctcgac catacgggca ataaacggtg ctgcaccaac ggttaacgga
    63061 acaattgctg cctgcaaacc aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc
    63121 cataccagca agataatgaa cgggatggaa cggaaaatgt tcacaatcgc agaaacggta
    63181 cgatacagct tcgcgttagc aataatttgc cccggacgcg tgacataaag caggacgcca
    63241 accggcagac caatcacaaa cccaaaaaat ccggatacga aggtcattgc cagcgtttcc
    63301 catacgccac gaaccagcag ccacatcatc ggctcagaca taacccagta cctctacttt
    63361 tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat
    63421 ttcagtcagc atgatgccga atttaacgcc accagcgtag tccatctgcg cgctaataat
    63481 gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc agtggggcat cgaccgattg
    63541 accggtaaac tccagacgca gcatcggcac gcagtcagtg aatggctccg cttgcagacg
    63601 ttcctggtaa tcttccggga tatccagatg cagggttgac tgaataaact tctgcgccag
    63661 cggcgttttc ggatgtgaga acacttcact taccgtgtcc tgctcgatta gttcgccatt
    63721 gctgatgacc gcaacgcaat cacaaatacg cttcacaacg tccatttcgt gggtgatcaa
    63781 cagaatcgtc aaacccagac ggcggttgat gtctttcagc agttcgagaa tagaacgtgt
    63841 cgttgccggg tccagcgcgc tggtggcttc atcacacagc aatactttgg gattgctggc
    63901 taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg gaaagattcg acgggtagct
    63961 atcatgctta tcgccaagac caaccaatga cagcaattcc gtcacgcgac gtttgatctc
    64021 gtctttcggt gtattgtcca gctccagcgg cagagccacg ttgccaaaaa cagtacgcga
    64081 agagagcagg ttaaaatgct ggaaaatcat accaatctgg cgacgagctt tggtcaactc
    64141 ggattctgac agcgttgtca gttcctggcc atcgaccagc acgctaccct cggttgggcg
    64201 ctccagcagg tttacacaac gtataagcgt actcttaccc gcgcctgagg caccgataac
    64261 gccataaatt tgtccagctg gcacatgcag gctgacgttg ttcaacgcct ggatgatgcg
    64321 ggtgccctgg tggaacactt tggtgatatt cgaaagtttt atcattgatt atttattatc
    64381 gtcattaagt tagtcgtggc atctcgaatg cctgaaacgg gcaacgccgt caatgaaatg
    64441 gatgttaagg catccagacg tctaaatcaa tcaggtttat gcgaagagca ctttcttgca
    64501 ggtcgaaaca tgcgatacta gcgtcacatg ccttattaag gagctataaa aggtggcgaa
    64561 gagcgtaccc gcaatttttc ttgaccgtga cggcactatt aatgtcgatc acggctatgt
    64621 ccatgagatc gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa
    64681 aaaaatgggc tttgcgctgg tggtagtaac caaccagtct ggcattgctc gcggtaaatt
    64741 taccgaagca cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgaga
    64801 tgtcgatctg gatggtatat attattgccc gcatcatccg cagggtagtg ttgaagagtt
    64861 tcgccaggtc tgcgattgcc gcaaaccaca tccggggatg cttttgtcag cacgcgatta
    64921 tttgcatatt gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc
    64981 agcggttgcg gcgaacgtgg gaacaaaagt gctggtgcgt acgggtaaac ctattacacc
    65041 tgaagcagaa aacgcggcag attgggtgtt aaatagcctg gcagacctgc cgcaagcgat
    65101 aaaaaagcag caaaaaccgg cgcaatgatt aaaagatgag cgattgaaat aaaaatgcat
    65161 ttttccgctt gtcttcctga gccgactccc tataatgcgc ctccatcgac acggcggatg
    65221 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaatcctgaa attcagggtt
    65281 tcctctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccacgt
    65341 cgcaactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc
    65401 ttaacgtcgc aaga
//
LOCUS       DAZFZC010000015        65371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFZC010000015 DAZFZC010000000
VERSION     DAZFZC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.015"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..717
                     /gene="comR"
                     /locus_tag="ACHKXP_001124"
     CDS             85..717
                     /gene="comR"
                     /locus_tag="ACHKXP_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO7646180.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(779..1318)
                     /gene="ycfJ"
                     /locus_tag="ACHKXP_001125"
     CDS             complement(779..1318)
                     /gene="ycfJ"
                     /locus_tag="ACHKXP_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7646181.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1545..2849)
                     /gene="ndh"
                     /locus_tag="ACHKXP_001126"
     CDS             complement(1545..2849)
                     /gene="ndh"
                     /locus_tag="ACHKXP_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO7646182.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3249..3791)
                     /gene="ycfP"
                     /locus_tag="ACHKXP_001127"
     CDS             complement(3249..3791)
                     /gene="ycfP"
                     /locus_tag="ACHKXP_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO7646183.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3814..4839)
                     /gene="nagZ"
                     /locus_tag="ACHKXP_001128"
     CDS             complement(3814..4839)
                     /gene="nagZ"
                     /locus_tag="ACHKXP_001128"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO7646184.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNRLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDML
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4850..5674)
                     /gene="thiK"
                     /locus_tag="ACHKXP_001129"
     CDS             complement(4850..5674)
                     /gene="thiK"
                     /locus_tag="ACHKXP_001129"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO7646185.1"
                     /translation="MPFRSNNPITRDELLSRFFPQYHPVTTFNSGRSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5655..6296)
                     /gene="lpoB"
                     /locus_tag="ACHKXP_001130"
     CDS             complement(5655..6296)
                     /gene="lpoB"
                     /locus_tag="ACHKXP_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO7646186.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6310..6687)
                     /gene="ycfL"
                     /locus_tag="ACHKXP_001131"
     CDS             complement(6310..6687)
                     /gene="ycfL"
                     /locus_tag="ACHKXP_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO7646187.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPFLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6690..7049)
                     /gene="hinT"
                     /locus_tag="ACHKXP_001132"
     CDS             complement(6690..7049)
                     /gene="hinT"
                     /locus_tag="ACHKXP_001132"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO7646188.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NIFIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7383..9572
                     /gene="fhuE"
                     /locus_tag="ACHKXP_001133"
     CDS             7383..9572
                     /gene="fhuE"
                     /locus_tag="ACHKXP_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO7646189.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQQFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(9632..11065)
                     /gene="ptsG"
                     /locus_tag="ACHKXP_001134"
     CDS             complement(9632..11065)
                     /gene="ptsG"
                     /locus_tag="ACHKXP_001134"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO7646190.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11360..12157)
                     /gene="ycfH"
                     /locus_tag="ACHKXP_001135"
     CDS             complement(11360..12157)
                     /gene="ycfH"
                     /locus_tag="ACHKXP_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7646191.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12168..13172)
                     /gene="holB"
                     /locus_tag="ACHKXP_001136"
     CDS             complement(12168..13172)
                     /gene="holB"
                     /locus_tag="ACHKXP_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO7646192.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13169..13810)
                     /gene="tmk"
                     /locus_tag="ACHKXP_001137"
     CDS             complement(13169..13810)
                     /gene="tmk"
                     /locus_tag="ACHKXP_001137"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7646193.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13800..14822)
                     /gene="yceG"
                     /locus_tag="ACHKXP_001138"
     CDS             complement(13800..14822)
                     /gene="yceG"
                     /locus_tag="ACHKXP_001138"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO7646194.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDKVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14825..15634)
                     /gene="pabC"
                     /locus_tag="ACHKXP_001139"
     CDS             complement(14825..15634)
                     /gene="pabC"
                     /locus_tag="ACHKXP_001139"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO7646195.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLVLSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15754..16995)
                     /gene="fabF"
                     /locus_tag="ACHKXP_001140"
     CDS             complement(15754..16995)
                     /gene="fabF"
                     /locus_tag="ACHKXP_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO7646196.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17083..17319)
                     /gene="acpP"
                     /locus_tag="ACHKXP_001141"
     CDS             complement(17083..17319)
                     /gene="acpP"
                     /locus_tag="ACHKXP_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7646197.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17530..18264)
                     /gene="fabG"
                     /locus_tag="ACHKXP_001142"
     CDS             complement(17530..18264)
                     /gene="fabG"
                     /locus_tag="ACHKXP_001142"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO7646198.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18277..19206)
                     /gene="fabD"
                     /locus_tag="ACHKXP_001143"
     CDS             complement(18277..19206)
                     /gene="fabD"
                     /locus_tag="ACHKXP_001143"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO7646199.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19222..20175)
                     /gene="fabH"
                     /locus_tag="ACHKXP_001144"
     CDS             complement(19222..20175)
                     /gene="fabH"
                     /locus_tag="ACHKXP_001144"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO7646200.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20243..21313)
                     /gene="plsX"
                     /locus_tag="ACHKXP_001145"
     CDS             complement(20243..21313)
                     /gene="plsX"
                     /locus_tag="ACHKXP_001145"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO7646201.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21394..21567)
                     /gene="rpmF"
                     /locus_tag="ACHKXP_001146"
     CDS             complement(21394..21567)
                     /gene="rpmF"
                     /locus_tag="ACHKXP_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO7646202.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21619..22140)
                     /gene="yceD"
                     /locus_tag="ACHKXP_001147"
     CDS             complement(21619..22140)
                     /gene="yceD"
                     /locus_tag="ACHKXP_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO7646203.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22339..22923
                     /gene="yceF"
                     /locus_tag="ACHKXP_001148"
     CDS             22339..22923
                     /gene="yceF"
                     /locus_tag="ACHKXP_001148"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO7646204.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKVQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVLFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23035..23994)
                     /gene="rluC"
                     /locus_tag="ACHKXP_001149"
     CDS             complement(23035..23994)
                     /gene="rluC"
                     /locus_tag="ACHKXP_001149"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO7646205.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24567..27752
                     /gene="rne"
                     /locus_tag="ACHKXP_001150"
     CDS             24567..27752
                     /gene="rne"
                     /locus_tag="ACHKXP_001150"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO7646206.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVTAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27948..28901)
                     /gene="flgL"
                     /locus_tag="ACHKXP_001151"
     CDS             complement(27948..28901)
                     /gene="flgL"
                     /locus_tag="ACHKXP_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO7646207.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28913..30556)
                     /gene="flgK"
                     /locus_tag="ACHKXP_001152"
     CDS             complement(28913..30556)
                     /gene="flgK"
                     /locus_tag="ACHKXP_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO7646208.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30622..31563)
                     /gene="flgJ"
                     /locus_tag="ACHKXP_001153"
     CDS             complement(30622..31563)
                     /gene="flgJ"
                     /locus_tag="ACHKXP_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO7646209.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31563..32660)
                     /gene="flgI"
                     /locus_tag="ACHKXP_001154"
     CDS             complement(31563..32660)
                     /gene="flgI"
                     /locus_tag="ACHKXP_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO7646210.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32672..33370)
                     /gene="flgH"
                     /locus_tag="ACHKXP_001155"
     CDS             complement(32672..33370)
                     /gene="flgH"
                     /locus_tag="ACHKXP_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO7646211.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33423..34205)
                     /gene="flgG"
                     /locus_tag="ACHKXP_001156"
     CDS             complement(33423..34205)
                     /gene="flgG"
                     /locus_tag="ACHKXP_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO7646212.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34377..35132)
                     /gene="flgF"
                     /locus_tag="ACHKXP_001157"
     CDS             complement(34377..35132)
                     /gene="flgF"
                     /locus_tag="ACHKXP_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO7646213.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35152..36360)
                     /gene="flgE"
                     /locus_tag="ACHKXP_001158"
     CDS             complement(35152..36360)
                     /gene="flgE"
                     /locus_tag="ACHKXP_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO7646214.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(36385..37080)
                     /gene="flgD"
                     /locus_tag="ACHKXP_001159"
     CDS             complement(36385..37080)
                     /gene="flgD"
                     /locus_tag="ACHKXP_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO7646215.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37092..37496)
                     /gene="flgC"
                     /locus_tag="ACHKXP_001160"
     CDS             complement(37092..37496)
                     /gene="flgC"
                     /locus_tag="ACHKXP_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO7646216.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37500..37916)
                     /gene="flgB"
                     /locus_tag="ACHKXP_001161"
     CDS             complement(37500..37916)
                     /gene="flgB"
                     /locus_tag="ACHKXP_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO7646217.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38071..38730
                     /gene="flgA"
                     /locus_tag="ACHKXP_001162"
     CDS             38071..38730
                     /gene="flgA"
                     /locus_tag="ACHKXP_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO7646218.1"
                     /translation="MLIIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38806..39099
                     /gene="flgM"
                     /locus_tag="ACHKXP_001163"
     CDS             38806..39099
                     /gene="flgM"
                     /locus_tag="ACHKXP_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO7646219.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39104..39520
                     /gene="flgN"
                     /locus_tag="ACHKXP_001164"
     CDS             39104..39520
                     /gene="flgN"
                     /locus_tag="ACHKXP_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO7646220.1"
                     /translation="MARLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39560..41095)
                     /gene="murJ"
                     /locus_tag="ACHKXP_001165"
     CDS             complement(39560..41095)
                     /gene="murJ"
                     /locus_tag="ACHKXP_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO7646221.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLVGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41205..42128)
                     /gene="yceM"
                     /locus_tag="ACHKXP_001166"
     CDS             complement(41205..42128)
                     /gene="yceM"
                     /locus_tag="ACHKXP_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7646222.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42130..42777)
                     /gene="yceH"
                     /locus_tag="ACHKXP_001167"
     CDS             complement(42130..42777)
                     /gene="yceH"
                     /locus_tag="ACHKXP_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO7646223.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     NRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42788..43372)
                     /gene="rimJ"
                     /locus_tag="ACHKXP_001168"
     CDS             complement(42788..43372)
                     /gene="rimJ"
                     /locus_tag="ACHKXP_001168"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7646224.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43608..44816
                     /gene="mdtH"
                     /locus_tag="ACHKXP_001169"
     CDS             43608..44816
                     /gene="mdtH"
                     /locus_tag="ACHKXP_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO7646225.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     TARRLLERDA"
     gene            44880..45527
                     /gene="grxB"
                     /locus_tag="ACHKXP_001170"
     CDS             44880..45527
                     /gene="grxB"
                     /locus_tag="ACHKXP_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO7646226.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45661..46221
                     /gene="yceB"
                     /locus_tag="ACHKXP_001171"
     CDS             45661..46221
                     /gene="yceB"
                     /locus_tag="ACHKXP_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7646227.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKVKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46327..47373
                     /gene="pyrC"
                     /locus_tag="ACHKXP_001172"
     CDS             46327..47373
                     /gene="pyrC"
                     /locus_tag="ACHKXP_001172"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO7646228.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESTCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47447..47692
                     /gene="dinI"
                     /locus_tag="ACHKXP_001173"
     CDS             47447..47692
                     /gene="dinI"
                     /locus_tag="ACHKXP_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO7646229.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47982..48236
                     /gene="bssS"
                     /locus_tag="ACHKXP_001174"
     CDS             47982..48236
                     /gene="bssS"
                     /locus_tag="ACHKXP_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO7646230.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48351..49469
                     /gene="solA"
                     /locus_tag="ACHKXP_001175"
     CDS             48351..49469
                     /gene="solA"
                     /locus_tag="ACHKXP_001175"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO7646231.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49517..49630
                     /locus_tag="ACHKXP_001176"
     CDS             49517..49630
                     /locus_tag="ACHKXP_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO7646232.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49891..50457
                     /gene="yceJ"
                     /locus_tag="ACHKXP_001177"
     CDS             49891..50457
                     /gene="yceJ"
                     /locus_tag="ACHKXP_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7646233.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50461..51036
                     /gene="yceI"
                     /locus_tag="ACHKXP_001178"
     CDS             50461..51036
                     /gene="yceI"
                     /locus_tag="ACHKXP_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO7646234.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51078..52130)
                     /gene="trhO"
                     /locus_tag="ACHKXP_001179"
     CDS             complement(51078..52130)
                     /gene="trhO"
                     /locus_tag="ACHKXP_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO7646235.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGASCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALSPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52355..53275
                     /gene="lpxL"
                     /locus_tag="ACHKXP_001180"
     CDS             52355..53275
                     /gene="lpxL"
                     /locus_tag="ACHKXP_001180"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO7646236.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53447..54673
                     /gene="mdtG"
                     /locus_tag="ACHKXP_001181"
     CDS             53447..54673
                     /gene="mdtG"
                     /locus_tag="ACHKXP_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO7646237.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            54756..55130
                     /gene="msyB"
                     /locus_tag="ACHKXP_001182"
     CDS             54756..55130
                     /gene="msyB"
                     /locus_tag="ACHKXP_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7646238.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55131..55358)
                     /gene="yceK"
                     /locus_tag="ACHKXP_001183"
     CDS             complement(55131..55358)
                     /gene="yceK"
                     /locus_tag="ACHKXP_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7646239.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55531..58074)
                     /gene="mdoH"
                     /locus_tag="ACHKXP_001184"
     CDS             complement(55531..58074)
                     /gene="mdoH"
                     /locus_tag="ACHKXP_001184"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO7646240.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(58067..59602)
                     /gene="mdoG"
                     /locus_tag="ACHKXP_001185"
     CDS             complement(58067..59602)
                     /gene="mdoG"
                     /locus_tag="ACHKXP_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO7646241.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYETPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            59996..61153
                     /gene="mdoC"
                     /locus_tag="ACHKXP_001186"
     CDS             59996..61153
                     /gene="mdoC"
                     /locus_tag="ACHKXP_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO7646242.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAES
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61161..62642)
                     /gene="clsC"
                     /locus_tag="ACHKXP_001187"
     CDS             complement(61161..62642)
                     /gene="clsC"
                     /locus_tag="ACHKXP_001187"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO7646243.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62584..63117)
                     /gene="ymdB"
                     /locus_tag="ACHKXP_001188"
     CDS             complement(62584..63117)
                     /gene="ymdB"
                     /locus_tag="ACHKXP_001188"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO7646244.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSFTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63212..63523)
                     /gene="ymdA"
                     /locus_tag="ACHKXP_001189"
     CDS             complement(63212..63523)
                     /gene="ymdA"
                     /locus_tag="ACHKXP_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO7646245.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTVQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63644..63976)
                     /gene="csgC"
                     /locus_tag="ACHKXP_001190"
     CDS             complement(63644..63976)
                     /gene="csgC"
                     /locus_tag="ACHKXP_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO7646246.1"
                     /translation="MNTLLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(64035..64490)
                     /gene="csgA"
                     /locus_tag="ACHKXP_001191"
     CDS             complement(64035..64490)
                     /gene="csgA"
                     /locus_tag="ACHKXP_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO7646247.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(64531..64986)
                     /gene="csgB"
                     /locus_tag="ACHKXP_001192"
     CDS             complement(64531..64986)
                     /gene="csgB"
                     /locus_tag="ACHKXP_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO7646248.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(65115..>65371)
                     /locus_tag="ACHKXP_001193"
     CDS             complement(65115..>65371)
                     /locus_tag="ACHKXP_001193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646249.1"
                     /translation="NVLVFSPHLRGQFFTAKRRGLSRLVRKTYRSNQRRARRMTVWCF
                     LIAENGDLKENKIFFCVDNSVFSWVLILWYELKKKNTTRG"
BASE COUNT        15975 a        17391 c        16028 g        15977 t
ORIGIN      
        1 atttttatgg gatctggact ggtgaacgga ggtgattatt tttataataa cctttcattc
       61 acagtcacca ggtacaacgg catcatggca actgactcaa cacaatgtgt aaaaaaaagc
      121 cgtggccgcc caaaagtgtt cgacagggat gccgcgcttg ataaggccat gaaattgttc
      181 tggcaacacg gttatgaagc gacttctctt gcggacctcg tcgaagcgac cggagccaaa
      241 gcgcccacgc tatacgcgga atttaccaac aaagaggggt tatttcgtgc cgttctcgac
      301 cgctatatcg atcgttttgc cgctaagcat gaagcacagc tgttttgtga agagaaaagc
      361 gtggagtctg cgctggctga ctattttgct gccatcgcca actgctttac cagcaaagac
      421 accccggcag gctgcttcat gatcaacaac tgcaccaccc tctccccaga ttcaggagat
      481 atcgccaata cgttgaaatc acgccatgcg atgcaagagc gcactttgca gcagttttta
      541 tgtcaacgac aagcgcgcgg ggaaatcccg acccactgtg acgtgacaca tctggcagaa
      601 ttccttaatt gtattattca ggggatgtcg atcagcgcac gcgaaggtgc atcgctggaa
      661 aaactaatgc agattgccgg aacgactttg cgtttatggc ccgaactggt gaaataagtc
      721 gcaaaaaaag cccctcagcc tgagcgaatg aggggccaaa ttgcagagta cagccttgtt
      781 atactttgtt attcaaaatc agttgcccat tactatctag cgggatctgc gtacccggat
      841 cgcggtccat gcggattttg ccctgctgat cgccaatctt ataggtcaca tcataaccga
      901 gcattttttc tgacttgtca tacaccgttt tacaacgctg ttgcgtagtc gtgtaagtat
      961 cgctttcctg gagagagccc tggatctggt taccggcata tccaccaccc agcgccccca
     1021 caacagtggc gacatcttta ccgcgaccac cgccaaactg atgccctatc acgccgccag
     1081 caacagcgcc gagcaccgac ccggtaatgc gattttcatc ctgcaccggt cgacgatggg
     1141 tcactgtgac gttgcgacac tcctgacgcg gtgttttaac cgtttccttg attggggttg
     1201 cagaaacaac ctgagcgtat tgcgggccac gttcaaacac gttcagactg gccactgccg
     1261 ctacgcccag cgcagctgcg acaccaatcc cgatacccgc caacattgat ttattcacgg
     1321 gaacacctcc ttctttgcag ttatgccaat catgcaagcg aaggagaaaa gcagctatca
     1381 gacagaagat gaaaagcgcg ggaatggctg gaaatactta atgattcgga ggagtctgaa
     1441 ggaatgcctg atgcgacgct taacgcgtct tatcaggcct accgctcgta ccatttgtcc
     1501 agggcggatg aggcgtttat gccacatccg ccagtgtacg tcgattaatg caacttcaaa
     1561 cgcggacgga taacgcggtt aatactcccc accagcatca ttaatccggt tttaaagtaa
     1621 ccatgcagcg caatctgatg cattcggtat agcgagatat atacaaagcg cgcaattcgt
     1681 ccttcaatca tcattgagcc gcgcgtcagg ttacccatca ggctaccaac ggtggagaag
     1741 ttcgacagcg ataccagtga accatgatct ttatactgat aatttttcag cggcttaccg
     1801 tttatctgcg ccagaatatt gttcattgcg caggtcgcca tctggtgtgc agcctgagca
     1861 cgcggcggaa caaagccccc ttccggacgc gggcatgacg cgcagtcgcc aatagcgtaa
     1921 atgtctggat cgcgggtggt ttgcagcgtc ggttccacca ccagctggtt gatacggtta
     1981 gtttcaagac caccgatatc tttcaggaag tctggcgctt tgatcccggc tgcccatacc
     2041 atcagatccg cctcaatata ttcgccatct ttagtgtgca ggccgccttc atcagcactg
     2101 gtgaccatgg tttgcgtcag cacgcgaacg ccaagtttcg ttagctcgtt gtgggccgca
     2161 gcagagatac gtggcggtaa cgcaggcaaa atacgttctc ccgcttctac cagcgttacg
     2221 ttcagggctt cgttggtcag gcctttgtaa ccgtagctgt gcagttgctt gaccgcgttg
     2281 tgcaattcag cggagagttc tacacccgtc gcgccgccgc cgacaatcgc aatgttcact
     2341 ttgccattcg cgcccaggtt ggcggagtat ttcaggaaca gattcagcat ctcctggtgg
     2401 aagcgacgcg cctggtgcgg gttatcgagg aaaatgcagt tctctttgac acctggtgta
     2461 ttgaaatcgt tagaggtgct acccagcgcc attaccagtg tgtcataggc gattttacgt
     2521 tccggaacca gcagttcacc tttctcgtcg cgcagttctg caatagtgat tgttttcgct
     2581 tctcgatcaa tatcaatgac ggaacccagc tggaactgga aaccatgatt gcgcgcatgg
     2641 gccagatagc tcaacgcatc gacgccttca tcaagcgagc cagtcgccac ttcgtgcagc
     2701 agcggtttcc acaggtggct gtggttacga tcgaccagcg taattttggc ttttttcttg
     2761 cgtcccagct tatgccccag ctgtgttgcc atttccagcc caccagcacc gccgccgaca
     2821 atcacaatct ttttcaatgg cgtagtcaac gtgaccccct taaatttatt aaccaattgt
     2881 taattaatgg ttattaacat agctcttaat taacaacagg ttacagcaat gaaaatgttc
     2941 ttcgctattg agaataacat gaatggtgca ttggtcatac caaaattgat gttaatcaag
     3001 ttttgttgct gaaaagatag gcgactttac cgggtgagat caaaaaacag agtttattgt
     3061 ttattatttg agcgaatttg tgagagtgat atagagtgag gggtgtatgc agagcgttcg
     3121 gtaggcctga taagaagcgc atcaggcatt gatgctcgac tgtcggatgc ggcgtggacg
     3181 ccttatccga cccacagaat tcggagcgca tcaggtaccg gcaaggttac ctgatgcgac
     3241 gagggcattt acccgagggt tttgaacgct ttaatgcgct gtaaatgcgg ggagatattc
     3301 ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg caactcttca
     3361 gatgtccgct ggctgttaag cgcttcatca ttacgcgaca aaatcaccag gcaacgatcg
     3421 cgattcttct cacggaagtt ggtcacacac ttagtggcaa tatcggcata ctcttccggg
     3481 cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa gatcacctgg
     3541 cggatgtcgc aaagaaaacc aatccgttcc gcccagtatc cgcccaagcc aacgccgcaa
     3601 attagcggac gctcgtcaac gttcagttgc aacattttgt ccacttcttt aagcagatgc
     3661 tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc cgggtcaata
     3721 aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa accgtgtaaa
     3781 tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct cttcctgcca
     3841 gcgttcatgt aactgattca gacgggtgct gatcgctttc cagcgagccg agtccatcag
     3901 ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg ccttgatcgg
     3961 tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca ggagcatatc
     4021 gcaacccgca tccagtgaag cctgcccgcg ttcggcataa ctgcccataa tcgcggcacc
     4081 ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa cccagttcct gacgcaaaac
     4141 ggttttcagc cagtagggag aaccgctcgc cggacgcgga tcaacatcac tgtagatcac
     4201 atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt aaggaactga agaccgacat
     4261 atctttagcg cgaatctccg cttgtggacg tggatcgcac ggtgtttctt tgtgtgagtc
     4321 tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc gttttcattc cggcttcatg
     4381 cataccatca ataaaccggc tggcaattgc cagggctttt tgtggatcgg catgataaga
     4441 acgctcgcca atcgccgcgc tgatatgccc gacatccagc acaggcgcaa agctgatatc
     4501 aatatccata gcgatcattt cgctggccat caaccaacct gcctcctgcg ccagtttgcc
     4561 accctcttcc attcctgaca gcgcagcgaa tgattgcgcc gctggcaagc gggtaaaacc
     4621 ttcacgaaaa cgctgcacgc gtccaccttc ctgatcaacc gccaccacca gacgattgcg
     4681 cgaagctgcg cggatctggc gcaccagttc acgtaactgg gcaggatcat gatagttacg
     4741 cgtaaagaga atcagccctc ccaccagcgg atgcgccagt atttcacgct cttccgcgtc
     4801 cagttcgtaa ccttcgacat ccaacattac tggacccaca ctgctctcct tattgtttta
     4861 ttaatagctg ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg ccggtttgtc
     4921 gccagcggta ctcaaaccac cctgctttga gcatcagcag ccagggaaac catcgcctga
     4981 cctgacgcca taattgcgcc ggataaatct tcgcgcgagt ggcatagtca ttgaccaatt
     5041 gccggtgctg ttcagtattt tccacccaca ccgccgccag ttccagcgcg atatcaccat
     5101 ctccggcata ctcccagtcg atgagtttta accctgacgc gctatgcact aaatttccgg
     5161 cgtggacatc catatgcaat ggacttaagc gtaaaggccg tggttcccgc gctttgcgca
     5221 gacgttttaa cattcgcagc caacccactg tccgccgcgc cggatcgctt tgctgccagt
     5281 acagttccag taacggcaac agcgttattc gccagccaaa acgtggttgt tgatgtagat
     5341 aatacagcaa gcctgccagt tcgttggtat ccggcaaata cgtttttacc gcgccgggca
     5401 gatagtcgac taccatccag tcacggagat ataaatgcgg cttcggtgca atgcatgcgg
     5461 gtagttgtga taaagcccga tactggcgca agaacgcgga ctgcggcgca tcaggatcgt
     5521 gcggctgacg cacaacaaaa cgctggccct gatgttcaat gagaaaactc ccgccactac
     5581 gcccactatt aaacgtcgtg acgggatgat actgcgggaa aaagcgcgac agcaattcgt
     5641 cgcgcgtgat gggattattg ctgcgaaacg gcacctttac ctgaccagat aatttcgccc
     5701 gtctgcacca gcatcagctg catttgtagg gtcggagcgt taacgttgcc agaggcgctg
     5761 gagtacagca cgtaatgagc gccgacattg cgggcaatgc ctatggcttt actacgggtg
     5821 cctaaactgt cctgcggcga caaacctaac tgttgcttcg ccatcgacag ttgctgggcg
     5881 gaaaccaggg taaatttccc gttattagcc agcgcatttc gcagcgtttc ggtcgcttct
     5941 gcggcattca gcgaaccgtt agtacggttg ttaacgctat caaccagcag gacgctaccc
     6001 gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg gctgcattgc gccattccag
     6061 tcataatggc gaatatgcgg cgcaggcggt gcagtttgat cttcgtgctc aattgggcct
     6121 ggctgctgcg ggatcgtcgg caccgagggc actgtgggga caggctgttg tggctcggct
     6181 ggttgttccg gcgctggttt cacttcttct accggtgcag gttcacgttg ccccacacac
     6241 ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc gactcatttt tgtcatcaag
     6301 attcacccct tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
     6361 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
     6421 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
     6481 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggaa
     6541 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
     6601 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acaccagaga
     6661 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
     6721 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
     6781 ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
     6841 gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgagac gtcgttcaca
     6901 gtcggaatga agatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
     6961 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
     7021 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
     7081 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
     7141 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
     7201 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacatgac
     7261 aaatatgaat gcgtatattt ctcatttgca tttacaaaca aaattattcg cacactaaat
     7321 ataatcgctc atcaccctcc tccggcatga gcctgacgac aacataaacc aagagatttc
     7381 aaatgctttc aacacaattt aacagggata atcaatatca agccatcacc aaaccgtcac
     7441 tacttgccgg ttgcatagca ctggcactat taccttctgc cgcttttgct gcaccagcca
     7501 ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatggc gaaaatgatt
     7561 acagcgtaac gtctacctct gcgggtacca aaatgcagat gactcaacgt gatattcctc
     7621 agtcggtcac tattgttagc cagcagcgga tggaagatca gcagttacaa acgctgggcg
     7681 aagtgatgga aaacacgctg gggatcagca aaagtcaggc ggattccgat cgtgctcttt
     7741 attattcccg cggattccag atcgataact atatggttga tggtatcccc acctattttg
     7801 aatcgcgctg gaatctgggc gacgcacttt ctgatatggc actctttgaa cgcgtagaag
     7861 tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg gcaattaata
     7921 tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga tgtctcggcg gaatacggta
     7981 gctggaacaa agaacggtat gtggcggatt tacaaagccc actcaccgaa gacggtaaaa
     8041 tccgcgcgcg aattgtcggc ggctaccaga ataacgactc atggctggac cgctacaaca
     8101 gtgaaaagac cttcttctcg ggcattgtcg atgctgattt aggcgatctt acgacgcttt
     8161 cagccggtta cgaatatcag cgcattgatg ttaatagccc tacctggggc ggtttaccgc
     8221 gctggaatac tgatggcagc agcaacagtt acgatcgcgc acgcagtacc gcacctgact
     8281 gggcgtacaa cgataaagag atcaacaagg tctttatgac cctgaagcag cagtttgctg
     8341 atacctggca agcgacactg aatgccaccc actctgaagt cgaatttgac agcaaaatga
     8401 tgtatgtcga tgcctatgta aacaaagcgg atggtatgct ggttgggcca tacagtaatt
     8461 atggacctgg ctttgattat gtcggcggca ccggttggaa cagtggcaaa cgtaaagttg
     8521 atgcgctgga tttgttcgct gacggtagtt atgaattgtt tggtcgtcag cacaatctaa
     8581 tgtttggtgg cagttacagc aaacaaaaca atcgttactt cagttcatgg gccaacatct
     8641 tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa accgactggt
     8701 caccacagag cctggcgcag gacgatacca cacatatgaa atcgttatat gctgccactc
     8761 gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc aactggcggg
     8821 ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct ggtctggtgt
     8881 ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc cagccgcaaa
     8941 atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac tacgagctgg
     9001 gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc ttccgtattg
     9061 agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac ggcgaaaccg
     9121 cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc aacggcgcaa
     9181 ttaccgacaa ctggcagctg acatttggcg caacgcgcta tattgcagag gataacgaag
     9241 gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc agctatcggt
     9301 tgcctgtcat gccagagttg acggtcggcg gtggtgttaa ctggcaaaat cgcgtgtata
     9361 ccgacaccgt gacgccgtat ggcaccttcc gcgccgagca aggtagctac gcgctggtgg
     9421 atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac gtcaataacc
     9481 tgttcgacaa aacctacgat accaacgtgg aaggttctat cgtctacggc acaccgcgta
     9541 atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag gcagccatct
     9601 ggctgcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac tcatccatct
     9661 cggttttcag gttatcggat ttagtaccga aaatcgcctg aacaccagaa ccagcaacca
     9721 ctacgcccgc tgcgcccagt ttcttcaggc cggcctgatc cactttagac acatcagcaa
     9781 cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct ttaccaccaa
     9841 atgcagcaac cagagccggt gccatttcgc tggtacctgt cgcttttgca tcttcagtcg
     9901 cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg aagatggtgt
     9961 agtaaacaat cgcataaccg ataccgacga tcgggaacag ccacagtttg ctgctgttac
    10021 cagacagaac gatgaagtcg atcagaccgt gcgagaacga cgtaccgtca cgcatcccca
    10081 gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg tacaggatcg
    10141 gcgcaacgaa catgaaggag aactcgatcg gctcggtgat accggtcagg aacgaggtca
    10201 gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta gcagagtgcc
    10261 agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca ccagacagtt
    10321 tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa acctgacctg
    10381 ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg tggtgcagac
    10441 caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact accgggttct
    10501 ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc caaatgaagg
    10561 acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca aagcgtttac
    10621 cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta aacatgtacg
    10681 ctgcgatcgc accggagata atccctccga gtacgccagt atccgccagg tgtttagagg
    10741 cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg gttttaacca
    10801 tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg gtaaagccga
    10861 gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg cctgcttctg
    10921 ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg acgcccagca
    10981 gaatacctgc gataggcagt acggataccg gcagcatcag cgatttaccg accttttgca
    11041 ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg tgcttttttt
    11101 acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc taagcgccct
    11161 ttatttatta cacagagtaa aataattcag tgccaatttg tttgacggct atcacgtttc
    11221 aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca atatttcaaa
    11281 cgtggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc cattaattac
    11341 gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg atgtgaaaca
    11401 gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca cctttcaaca
    11461 cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt cctcgatgcg
    11521 gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa ccgatccagg gggacataac
    11581 gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac aatgccggaa aaggagatgt
    11641 aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa cagtgtagta
    11701 cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg gcacgggcgt
    11761 cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata tgatggatga
    11821 aggactcttg ctgacgtact ttagtttccg gcgtgtaata ataatccagc ccggtttcac
    11881 ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca tcgtagggat
    11941 cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt tcgcctacca
    12001 gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa aatttcacat
    12061 cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat tcataatcca
    12121 gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta aagatgagga
    12181 accggtagca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag atcggtgatg
    12241 agaagctcgc ggttgatgcc tgtaacagac attaactgtt cacgaatgtg gcaaacatcc
    12301 cccagtatag cctgcaggcg cgagggagaa agatggtttg ccagttcggc gaccaggccc
    12361 ggcacatcaa cattggtcac ctgcgcagca ccatgatggc gttttagcgc atccatcagc
    12421 aacgttgcca gccagtgtaa acgcgccgga gcttgttcat gattaagggc cgctagcagc
    12481 gaataccagt cgcccgatgg cacgctatat gccaacgcct gacacaatgt ttcacgagcc
    12541 tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc gcttaagcgc
    12601 aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca ggtcacggcg
    12661 tactgttccg gcggcggcgc aaggtaatgt aaccgacaac gactacgtaa tgttgccagt
    12721 aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg ctcttcaagc
    12781 gttttcagca atgcgttagc cgcggcgtcg gttagtaagg cagcatcggt tacccaaacg
    12841 actttcgcac cacctaagcg tgcgtgctca ttcagctttt cggtgacctc acgtaccgca
    12901 tcaacgccca gcgtattttt tcctttttcg ggagccaggg tgtagtaatc gggatgcgtg
    12961 ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc ctgcggttgt
    13021 tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat gcccggtaac
    13081 gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac cagtttttcg
    13141 aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc cagtgggtca
    13201 cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca atggtatgaa
    13261 tgcttttatc ttgtgctgcc agttccagat agcgggcgcg ggtgcgatta aagaaatcga
    13321 aagattcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt aagccaactt
    13381 ccggggtaac atcgagatag agcgttaagt cggggcgaaa atccccgaga acagcatcac
    13441 gcagtgttgc cagcatatgt tggtcaatac cacgtccgcc gccctgatac gcctgagtgg
    13501 agagatcgtg gcgatcgcca atcacccagg tgccgttagc cagagctggt ttgatgaccg
    13561 tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta tcggtaatga
    13621 cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg gcaagttgcg
    13681 taccgccagg ttcccgagtg aaaaccatgt cgcggatacc cagttgctcg agcgtctcaa
    13741 ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg acgatatact
    13801 tactgcgcat ttttttcctt aagcactttc agataatcct gcacagactt gttatggctg
    13861 gcaagattgg tattaaacgt gtgaccacct ttaccatcgg ccacaaaata gagatacggc
    13921 gtttttgccg gatgcgcagc agccttcagc gaatccgccc ccggcgtcgc tatcgcacct
    13981 ggcggcagac cggtaatggt ataggtgtta tacgctgtcg gcgtttccag gtctgcacga
    14041 gaaagtttgc cattataacg ctctcccatc ccgtaaatca cggtcgggtc ggtctgcagg
    14101 cgcataccaa tgcgtaaacg gttgataaat actgaggcaa ccttatcgcg ttcactggca
    14161 acggcggttt ctttttcgat aattgatgcc atcgtcacca actggttttt atctttataa
    14221 ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat tttcttgtgc
    14281 gctcgcttga gtaacgcgac atcggtggta ttggcggtat acatccaggt gtctggccag
    14341 aaccaacctt caatccactc cgggttttcc agttcaagtg cctgcgctac ggtggcgtac
    14401 ttatcatcgc tcagcgtatg cttgatatac ggggcctcac gcaattgctt gaggtaatcg
    14461 ctcagacgca tcccttctac cagtcgcaga gggaactgtg cttctttacc gctttccagc
    14521 aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc ggctttaaag
    14581 tgagaaagat ccggttcgat acgcagcagc cattgaaaaa cccgtggacg attgatgatc
    14641 ttatcggcat aaagctgttc accgagcgcc agacgtccgg tccctggctt cagggtaaat
    14701 atcgtctctt ctttgataag caatttgctg tcggcaagat ggcgaacctt ccagacgccc
    14761 acaccagcgg cgatacccag taccaccagc aataacaaga ttatcaataa cacttttttc
    14821 atgactaatt cgggcgctca caaagtgggg ctaaatattc atataacgtt gctgacgaaa
    14881 aggagacatc gccacaggca cgtacgggca tcactggcat taacgcatta caaataacca
    14941 tctcatctgc ctgcaacgcc tcttccagag aagcttgcac ttcgacaagc tgataagagg
    15001 attgtgccag caaacggata cagaattgtc gcataatgcc gttaacacct gcctgatcca
    15061 gtcgcggcgt ataaactacg ttgcccttcc gccagaacaa attagccgca cagcattccg
    15121 taacccaccc ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc tcaagatgag
    15181 agcgaatcaa tacttgctca agccgattaa gatgtttaat acctgcaaga tgaggattgc
    15241 gccccagccg caccgggctt agcaccaacg tcatcccctc gttacgcaaa cggtcgtaat
    15301 gtgcaggata agccgtaacg gagagaatcc gcgttgctgg tccgctgttc aatgtgctgt
    15361 accctcgccc gccactaccg cgactgatca cgactttcag tacaccattt tgctgttctg
    15421 ctgccagcgt tttcatctct tgttcaagct gaggccagaa gtcacaggaa atcatcaacc
    15481 gctgacaagc atcctgtagt cgctggatat gcgccgataa caaactgact ttaccgtcga
    15541 taactctggc agtggtaaaa caaccatcac caaactgcgt tgcccgatcg cttactgcca
    15601 gcgattcctg cttataaccg ttaattaaga acataccggc tccttatcag agaattgtta
    15661 gtgtggcagc atgttcacta cggaacaagt cggaataaaa gctaagaaaa aaggcccgca
    15721 agcggacctt ttatatgggt gggaaatgac aacttagatc tttttaaaga tcaaagaacc
    15781 attagtgcca ccgaagccga aggagttaca cagcgtgtat tccattccgc tgacctgacg
    15841 tgcttcatgc ggtacgaaat ccagatcgca accttcatcc gggttatcca ggttgatggt
    15901 tggcggaaca gcctgatcgc gcagcgccag gatggagtag atagactcta ctgcccccgc
    15961 cgcacctaac aggtgaccgg tcatagattt cgtggagctt accaacacac ggcttgcagc
    16021 ttcaccgaag atggctttca ccgcctgcgc ttcagcttta tcgccagccg gcgtagaagt
    16081 accgtgcgcg ttaacgtagc caatctgact cgcttcaatg cctgcatcac gcagagcatt
    16141 tgccattgcc agagctgcgc ctgcgccatt ttctggcggt gacgtcatat gataagcatc
    16201 gctgctcata ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt ttttcgcgtg
    16261 ttcgtactct tcaagtacca gcataccggc accatcgccc agtacgaaac catcacgctc
    16321 tttatcccac gggcggctcg ccgcttgcgg gttatcattg cgggtagata atgcacgtgc
    16381 cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc tctgcgccac ctgcaaccat
    16441 cacgtcagca tcgccatacg cgataatacg cgcagcatgg ccaatgttgt gcacgccgga
    16501 agtacaggca gtcgcgatag agatgctcgg gccacgcagg ccatacatga tagtcagatg
    16561 acctgccacc atgttcacaa tcgttgacgg aacgaagaat gggctgatct tacgtggacc
    16621 accgttcatc agagatgtgt ggttttcttc gatcagtccg aggccgccaa tcccggagcc
    16681 aattgcggca ccaatgcggg ttgcgttctc ttccgttatt tcaaggccag aatcctgcat
    16741 ggcctgaacg ccagcgacaa ttccatattg aatgaaggca tccatcttgc gctgttcttt
    16801 gcgcgagata atgtcctcac agttaaaatc ctttactaag ccagcaaatt tcgttgcata
    16861 ggcgctagta tcgaaatggt cgattaggct gatgccactc tgaccggcaa gcagagcttt
    16921 ccaggtagac tctacggtat tgccgacagg agacaacatg cccagtccgg tcacaactac
    16981 acgacgctta gacacgtttg tcctccaggg agggaaaaaa tgattctagt gggacaaaaa
    17041 gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct ggtggccgtt
    17101 gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt ccggaatctc
    17161 agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag aatccgcgcc
    17221 caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc ccagctgttc
    17281 gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt tcctatcaaa
    17341 actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc aactaaatcc
    17401 cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat aacgcaaatc
    17461 attttgcact aattattact cataaccacg cagactgcgc gcaacatgag cattttgtgc
    17521 aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac ccgtgatgta
    17581 agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg cgccgccgag
    17641 gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca gcgcacgtgt
    17701 catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc gtgacgcaac
    17761 ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag cgtagttggc
    17821 ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac gaccatgacg
    17881 ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg aaaggttggt
    17941 ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat cacgagtgat
    18001 accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt tttccagaac
    18061 agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt tggcacctaa
    18121 ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa ctttcgcgcc
    18181 acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac cggttaccag
    18241 tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcgagcg ccgctgccat
    18301 cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt tcgtcaggcc
    18361 agtaagcact ttgcccgggc cgacttcata gagatgttct acgccttgcg ctgccatgta
    18421 ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg cgtcacggat
    18481 ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacaggaa ctgttggtgc
    18541 gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt tcatcagcgc
    18601 acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt tttgcgcccg ccgctttaca
    18661 ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct gtcccggaga
    18721 gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg ctttcgcaat
    18781 agacgcatca tccagaccga tgattgccgc catagcgccc gtgccttccg gtacggcttc
    18841 ttgcatgaac ttgccgcgca tctcaaccag acgcaccgca tcagcgaaat caatcacacc
    18901 agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg ccggtgcttt
    18961 accgccctgc tgctgccata cgcgatacag cgcaacagat gcagtcaaca gcgcaggctg
    19021 agtttgccag gttttattca gttcttcagc tggcccctgc tgggtcagcg cccacaggtc
    19081 gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat agctcgccgc
    19141 catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa atgcaaattg
    19201 cgtcatgttt taatccttat cctagaaacg aaccagcgcg gagccccagg tgaatccacc
    19261 gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc gtacagcttc
    19321 atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca gcgtcaccac
    19381 gacattatcc atagacatac cgagtttttt cgccgttgca ctgataatac gcaggttagc
    19441 ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg ccagcgtctc
    19501 atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc ctgccatcgt
    19561 cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca gcaattcacc
    19621 ataactaccg tcggcatgca gatgggtgga aatgattccc ggctcttcag aggcagccag
    19681 caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatcgg ttggatcgca
    19741 ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc cagatttcac
    19801 gtattgatcg gctacgctta atgcataggt gaaacctgcg caggctgctg caacgtcaaa
    19861 tgccgggcaa cctttaatgc ccaacatgct ttgaatctga caagctgcgc tcgggaaagc
    19921 gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct caatgcccgc
    19981 catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt cgtttggcgc
    20041 ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag aggtgtccac
    20101 cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc cagtaccaat
    20161 aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac ttgcaaactg
    20221 cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg tccagcagct
    20281 caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga acttgtcgct
    20341 gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg gctgcaccat
    20401 gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac tggtcggggt
    20461 tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat aacagtagcc
    20521 accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac atcctgacaa
    20581 caccttccat cgtctttaat gtgacatttc ctgtaaagcc gtcacaaacc agcacatctg
    20641 tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata gaagggattg
    20701 ttttaagcac cgctgaggca tcccgaatac tgtcgagacc ctttacttct tcttcaccaa
    20761 tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc agaactgagc
    20821 ccataatggc aaattgcacc agcattgtgc tgtcacaatc gacgttggcc cctaagtcaa
    20881 ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc ggacgctcaa
    20941 tcccctccag gggcttgagt aataattttg ccagccccat cagcgccccg gtattaccgg
    21001 cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccacg cgcattgaac
    21061 tcccacgact ggcgcggata gcttgcgaag gccgggcatc actggcgata actgactgcg
    21121 caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt aatggcgtga
    21181 tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc agtgcctgca
    21241 atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct aacgccaggg
    21301 ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg cctggggaaa
    21361 atcctcacta agcttcatca cgcagatgcg tgattactta gcgatgacct tgcggccgcg
    21421 gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag ttttgtctac
    21481 agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac gtttggaacg
    21541 ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt ttatccccga
    21601 tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa atgggtttgg
    21661 cttttgcgct tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca cttcacagtg
    21721 ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt caaccattgc
    21781 aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt ccggcagtgc
    21841 ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga cctgatgagt
    21901 aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat cgccgtttaa
    21961 cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat cactgtccac
    22021 actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac cctggtaatc
    22081 aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta ccttttgcat
    22141 agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag cctgtggttg
    22201 ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt cagtgctgat
    22261 aacaaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat caatacgcta
    22321 tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc atggcgtcgc
    22381 gctcttctgg aaaaactgca aatctctttt gaatgtgcag caccagaggt cgacgaaacc
    22441 ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga aaaagtgcaa
    22501 tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt atgtgttctt
    22561 gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca attacgcaaa
    22621 gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc ggcgaatggg
    22681 catctgcaaa cagaagtgga gccttttgac gtccttttcc gtcacctgag cgaggcggag
    22741 attgataatt acgtgcgtaa agagcatccc ctgcactgcg cgggtagctt taagagtgaa
    22801 ggatttggca ttacgctgtt tgagcgttta gaggggcgtg atcctaacac gctggttggt
    22861 ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc gctgatggga
    22921 tagttttttg cgccggatgt ggttacgtcg catccggcaa tcggtgctgg cggcctgatg
    22981 cggcgtaaac gccttatcag gccaaccgcc cattatcagg tttatatgct tgtattagcg
    23041 cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggag cttcgatacg
    23101 catcacctca ccggtccccg gatgagtaaa cttcaacgct gcagcgtgca ggaacaggcg
    23161 atttaatccc gtgcctgctt cagtgagctg cctgtcaaat tcacggtcac cgtagcgatc
    23221 gtcaaaggca atcggatgac ccgcatattg tgtatgcaca cggatctggt gagtacgccc
    23281 tgttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca ctttaaagcg
    23341 tgtttccgac ggtttgcctt cctgactcac acgcacgata cgttcgccgc tttgcagaat
    23401 atttttcagt aacggtgctt gaacgctctt cacatgcgac tgccactgac cgcgcaccag
    23461 cgccaggtaa tctttttgca tccctttttc acgtaattgc tcatgcagag aacgcaacgc
    23521 cgagcgtttt ttcgctacca gcaaaacacc tgaggtgtcc cggtcaagac gatgaaccag
    23581 ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcaataacgc cgaagcttaa
    23641 accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga tgtgatcatc
    23701 ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg aaaccgcctc
    23761 ttcttcccgc tcagcaacgc gaaccggtgg aatacgcacc tcatcacccg cttcgagttt
    23821 atattcaggc ttaatacgtt ttttgttcac ccgcacttcg cctttacgca aaatacggta
    23881 aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga tacgttgccc
    23941 cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt tcatggttgg
    24001 cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat atttactttt
    24061 gcaccttatt acttcactgc gtgatcactt tattgatggt tattaaacca atcaccagca
    24121 agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct tgctataaca
    24181 aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa caagaaattt
    24241 tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta agacgtattg
    24301 atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg gaaatatttc
    24361 tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc atgaaaaacg
    24421 ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg cgacgcgact
    24481 agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag cttagtcgtc
    24541 aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca actcagcagg
    24601 aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat atcgaaagtc
    24661 cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc attgaaccga
    24721 gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc ccactaaaag
    24781 aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc aacattaaag
    24841 atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag cgcggcaaca
    24901 aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt ctgatgccga
    24961 acaacccgcg cgcgggtggc atttctcgcc gtatcgaagg cgacgaccgt accgaattaa
    25021 aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg cgcaccgctg
    25081 gcgtcggcaa atctgctgag gcgctgcaat gggatttaag cttccgtctg aaacactggg
    25141 aagccatcaa aaaagccgct gaaagccgcc cggccccgtt cctgattcat caggagagca
    25201 acgtaatcgt tcgcgcattc cgcgattact tacgtcagga catcggcgaa atccttatcg
    25261 ataacccgaa agtgctcgaa ctggcacgtc agcatatcgc tgcattaggt cgcccggatt
    25321 tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac taccagatcg
    25381 agtcacagat cgagtccgcc ttccagcgtg aagttcgtct gccgtctggt ggttccattg
    25441 ttatcgacag caccgaagcg ttaacggcca tcgacatcaa ctccgcacgc gcgacccgcg
    25501 gcggcgatat cgaagaaacc gcgtttaaca ctaacctcga agctgccgat gagattgctc
    25561 gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc gacatgacgc
    25621 cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt caggaccgtg
    25681 cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc cgtcagcgcc
    25741 tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc ggtaccggta
    25801 ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tctgatcgaa gaagaagcgc
    25861 tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct tcttatctgc
    25921 tgaatgaaaa acgttctgcg gtgaatgcca ttgaaacgcg tcaggacggt gttcgctgcg
    25981 tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgctgcgc gtgcgtaaag
    26041 gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa gcgatggcgc
    26101 tgccgtctga agaagagttc gctgaacgta agcgtccgga acaacctgcg ctggcaacct
    26161 ttgccatgcc ggatgtgccg ccagcgccaa ccccagctga acctgccgcg cctgtcgtag
    26221 ccccagcacc taaatctgca ccggcaacac cagccgctcc tgcccaacct gggctgttga
    26281 gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaaacc aaaccgtccg
    26341 agcaaccaac accgaaagca gaagcgaaac cggaacgtca acaggatcgt cgcaagcctc
    26401 gtcagaacaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa cgtactgaag
    26461 gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag cagactgccg
    26521 agacgcgtga gagccgtcag caggctgagg taacggaaaa agcgcgtacc accgacgagc
    26581 agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaatga tgataaacgt caggcgcaac
    26641 aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa caggaagaac
    26701 gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa gtgcgttacg
    26761 agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact gtcactgccg
    26821 aaccaattgt tcaggaagcg ccagctccac gcacagaact ggtgaaagtc ccgctgccag
    26881 tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac cgtgacaacg
    26941 gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtaagt ggtcagcgtc
    27001 gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg accgtagcgt
    27061 gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt gtacgtccgc
    27121 aagatgtaca ggttgaagag cagcgcgaac aggaagaagt acatgtgcag ccgatggtga
    27181 ctgaggtccc tgtcgccgcc gctatcgaac cggttgttag cgcgccagtt gttgaagaag
    27241 tggccggtgt cgtagaagcc cccgttcagg ttgccgaacc gcaaccggaa gtggttgaaa
    27301 cgacgcatcc tgaagtgatc gctgccgcgg taactgaaca gccgcaggtg attaccgagt
    27361 ctgatgttgc cgtagcccag gaagttgcag aacaagcaga accggtggtt gaaccgcagg
    27421 aagagacggc agatattgaa gaagttgtcg aaactgctga ggttgtagtt gctgaacctg
    27481 aagttgttgc tcaacctgcc gcgccagtag tcgctgaagt cgcagcagaa gttgaaacgg
    27541 tagctgcggt cgaacctgag gtcaccgttg agcataacca cgctaccgcg ccaatgacgc
    27601 gcgctccagc accggaatat gttccggagg caccgcgtca cagtgactgg cagcgcccta
    27661 cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac ggcaacacat catgcctctg
    27721 ccgctcctgc gcgtccgcaa cctgttgagt aataattagc tcaaagtaat caagccctgg
    27781 taactgccag ggctttttta tttcatcttt gaatcaacat attaaaattc atcgaatggc
    27841 atccttgcta accaacaatg caaaataggc aaaaaaatgc ccgccccagc gatcccagag
    27901 caggcagaca aaacataccc agtttcatga tatgttttaa agcgaaatta tttgttgagc
    27961 tggaagagcg acaatccctg catatcggta aatgctttat acgatgcctg caatgccgtt
    28021 tgctgcatga tgtaagatga aatagttgca ttccagtcca catcaaccag atcgctcatc
    28081 tgctgcgttt gccctaaagc gcgatcgcta cctaatgaat ccagcgactc cagttcgttc
    28141 agctgcgtgc ctaattccgc acgaacagtc agcacattgt tcagcgagtt tttcagtccg
    28201 cggttggttt tatctaacgc cgcagcggcg gtttctttat ccgcttcgct atccgcgacc
    28261 ggcgttttca gggctgcgat ggcactatcc agcatggcaa aaagattggt ttcagaagcg
    28321 ctaccgtctg gttccgctac cgcgttgctg gtaatactgt cgaaaatttt gtcacccgtg
    28381 tgccctatca ccatcgaacg cgaagcatcg acctgttgct taatactttc tgttccgccg
    28441 acataatcac cgttagcctc actaaacggc gcagtttctg ttttataacc ggcaaaaatg
    28501 taacgcccgt taccgtcagt ggtgttcgcc agattcagca actggtcacg aagcccctga
    28561 atatccgtag ccagcgaggc ccggtcatcg tcactcaagg tgccattgct ggcgtagaca
    28621 attttttcct gagcattctg gatagcagtg gtgacctggc taagtacact ctcttccagt
    28681 gacacttttt gagtggcgaa agtacgtgcc agcgtgtact ggctgttttg cgcctgtgcc
    28741 tgggagagaa ctacggcttg tgatgctgca atgggatcgt cagaagggtt aacgactcgc
    28801 ttacctgtcg acatctgctc gccgtacttc atccattctg cctgagaatt ggtgatgcca
    28861 cgcatgtttt gctggtacat catctgggta ctgaaacgca tcttatctcc ccttagcgaa
    28921 tgttaatcag cgcatcaaaa atcgcgtttg ccgtctgcag aacctgcgca ttcgccaggt
    28981 aatactgctg aaaacgttgc agatttccgt actcctcatc gagattgaca ccggaaatcg
    29041 actgctgctg attggaaagc tgcgtcacca cattaccttg cgtggtgcta ctggttttca
    29101 acgtcgcggt tttattaccg atatcactca ctaacgaggc ataagcgtcg ttaaaggatt
    29161 tcgcaccgcc caccgtttta ctgttgcttt gcagatccag cagggcctga ccgttgcggt
    29221 tgtcgctatc acccgcatct tcttcgctcg ccatcgcgat tttcgcttcg tcggtgatta
    29281 atacatccat gttgacgatg gcgtcactta ctggtttcag cgtgaagctg tcgttaacgg
    29341 caggcgttcc tgtaaacgtc aactccagac catcaaatgc cactttaccg ttggcatccg
    29401 gcgtcaccgt aaaagtggta ttgctggcaa ggcgggtgac ctgccactga ttattatcga
    29461 acgagatttt gtaatctgtc gccagtaccg cggaggcatc agttaccgtg gcaccgatcg
    29521 caacgtcacc tttgtttttc gtgttttgca gaaccgcggg cttaccgata gcaaagaaat
    29581 cttcaccggc atcgccgtta gcatcaaatc cggctttgtg ttgggtgttg aaagcctcgg
    29641 caaatgccag cgccagttgt ccaagcgtat tacgcgtctg gtccagatcc tgagaacgga
    29701 atgtcagaat gccgcccagc gacccggtat tcagtaattt ctccgggatc tcaatattgc
    29761 ctgccgtccc atcaacataa gcgacagtcg tacgagaagg gtcagcgctg gaaggaactg
    29821 ccgccagttg ccgcgccgta cttccctgaa ccagtgagta accattggcc atcgtgatgt
    29881 tataagtgcc gccatcctga acgctgactt ctacaccaac aatctggttt aattcgctca
    29941 ccagttgatc gcgttgatcc agcagattgt taggtgacgc ccctgccccc acgcctgtca
    30001 ggcgcgagat ttgatcgttc aggctggcaa tttgtttagc gtagttgttg atctgatcaa
    30061 cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc gcgcagatat tgatcggtgg
    30121 ttttaaactg attcaccaat ccttctgatt tcccaatcag cgcctggcgc gctgccgggt
    30181 cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa atcctgcatc tgtgttgcca
    30241 gcgaagaggt actggtggag agcatattgt cgattttcga catctgctca tagcgggcag
    30301 tcagaccgct actttgcgtc tgcgccgcac gtaactggtt ggtaataaac gcatcatact
    30361 cacgctgcac accagaaacg tagacaccat tgccaaccca gccgccagcg cccaacgtgc
    30421 tattggcctg cgccataata gtggtttggc gggtatatcc ggcaacgtta tagctggaga
    30481 tattattact tgccgtattt aacgccgcct gggccgcgtt cagtccgctc atggcgttat
    30541 taatcaagct ggacatggag gttccttata agccttcaat tacagagtat tatcggcagc
    30601 gacccgccgg acttgagtta ttcagaacag attatcaatg ttcatactgt aggttttgct
    30661 caccttgtcg cttatcgatt tcatctgctg aatcatgttg gtgagtttgc gggcatagtg
    30721 aggatcggtg gcatagcccg cgtcctgtag ggcctgcgcc ccctgttccg cactcgcggc
    30781 ggtcgtcacg gcggcgtagc gcgggttacg cgttaacagc ccaacgtaat ccgacaaggc
    30841 ttccagatac gagctgtaga cgcgaaactt cgcttttact ttcttcgctt cgccgttttc
    30901 atattcagtc gtggtgattt cagtaactgg ccctttccag ttgccagagg ctttgacacc
    30961 aaacaggtta tagctcggct cgccgttttc gcggcggatt tgccgttgcc cccaaccaga
    31021 ttccagtgcc gcctgagcga ggatcaaatg atgtggcaca ccgctttgct ggcttgccag
    31081 ttgggcgggc agcgagagtt gcgcgaggaa tgctttactg tcacccggca gcgaatcatc
    31141 gtagttacgt ggcacggcct tttgcaccag ctgcgaaagc gcctgatttt gataacgcac
    31201 cacagtttcg agcgggaatt tcatcggtgc tgctggcgtg gactcctctg gcaatggttg
    31261 ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc agacctttgc ccgccgtcat
    31321 ctgttgggca atctgctggt catacatact ggtatacagg cgagtgtgct cgctgctgaa
    31381 caggccatct tttggtaaag cgtcgcgcat gcttttcaac atcatctgca cgaacatccc
    31441 ttccacctga cgggccaccg gacggatatt tgccgccgga tcttcgcccg ctttcgcctt
    31501 tagttcgttg agtgattgcg catcccaggc cgcacttgcc agtagtttgc tgtcgctgat
    31561 catcagatga tttccagttt tgcccgcaga catcccgcac tttgcattga ttgcagtatg
    31621 gacatcagat ccatcggcgt agcgcccagc gcattgagcg cgcgcaccac gttattgagg
    31681 ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc gatctgcgtt
    31741 tgtggagtaa ccacagtctg tccaccacca aacggtgtat ctggctggct gacattggcc
    31801 tgacgattaa ctgttactga gagattcccc tgcgctaccg cgcagctgtc gagggtcact
    31861 tcgcgattca tcaccaccga accggtgcgc gagttaatca ctactttagc gtcctgcggg
    31921 gtgacattaa cctgcatatt ctgaatatcg gcaaggaagc ggacctggga actgttgcca
    31981 ctcggtacgc gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc atatccacgc
    32041 acgcggttga tggtgtcagc gatttgctgc gccatgctga aatcttcgtc gttaagttgc
    32101 aaattaaggg tattcccgac gccaaactgg ctgggcaatt cacgttcaat aaccgcacca
    32161 ttggtgatcc gtccaccgtt cagttggtta acctgaacac tgctaccgcc agcggaggct
    32221 cctgcgccgc caaccagaat attgccctgc gccagcgcat acacctgact gtcaacgccc
    32281 ttaagcggtg tcatcaacaa cgtacctcca cgcaagcttt tggcatttcc catggaagaa
    32341 accaccacat cgatggtttg cccctgacgt ccaaacggag gaagtgacgc tgtcaccatt
    32401 accgcagcga cgttttttag ctgcatattg gtgcccgtcg gaacggtaat tcccagctgt
    32461 gagagcatgt tattaagcgt ttgtgtggta aacggcgtct gggttgtctg gtcaccggtg
    32521 ccatccagcc ccaccaccag accatagcca atcagtgagt tttgccttac cccctgaaca
    32581 ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg tgaccagtag aagaattaat
    32641 gcagagagaa atttaatcac cacagcctca cttacattgg cgacaggtta aggaagaaac
    32701 gctgcaacca gcccatattt tgcgcttcgt taatgtagcc attgcctacg tattcaatgc
    32761 gcgcatccgc cacctgagta gacggtacgg tattgctgcc gctgatagtg cgtggattaa
    32821 ccacgccaga gaagcgaata aattcggtac cctgattaat ggcaatctgt ttttcaccca
    32881 ccacatgcag gttgccgttg accagtacct ggtcaaccgt caccgtcaac gtgccgctaa
    32941 aggtattgct ggcattggcc ccgccctttc cgttgaacgt gttaccaccg gaggcttcga
    33001 catcggcacg agcgttacca aacagcccct gcaaatagcg cggcacagta tcaaagccaa
    33061 aattagtttt accgtcacgg ctggcattcg cagaggagct tttgctggcg ctgacgttct
    33121 cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct tcaaacagcg
    33181 gttgatagcc atagttaatc ggctgagcag actggaaaat agaaccgttg gcgacgggcg
    33241 tcggaccggg aaccggttgt gcactggtcg ccccctgcac cagcggcgtg gagggtatcc
    33301 aggcgcagcc ggttagtgaa agcaccaaca agctggaaat ggcataagta tgcgcagcgt
    33361 ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc accggttaag
    33421 ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg tggacaccgc tttactgttg
    33481 atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt cttccgccac gttgacgtta
    33541 gacgtttcaa catacccttg atacagcagt cccgcgccgt tcaggcccgg cgtgctttcg
    33601 ttcggtgcac cagaggattg cgtttcggtg tagaggtttt cgccaatgct ctccagcccg
    33661 gtgtcattca taaaggtggt gagattgagc tgcccaacct gaaccggagc tgcctggcct
    33721 tgttgggtta cgctgaccac gccatcacga ccgatggtga tacttaacgc attcgccgga
    33781 atggtgatcg ctggctgcac ctgaaaacca ccagccgtca ccagctgccc gttctgatcc
    33841 acctggaaag agccgtcacg ggtataggct gatgaaccat ctggcaacat cacctggaaa
    33901 aagccctgcc ctttaatcgc gacatctttg ctgttgttgg tctgcgacag gtttccctgg
    33961 ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc cgatttgtaa tccggagggt
    34021 aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa tggtttgata aagcagatct
    34081 tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga cgtttgccag gttgttggca
    34141 atgacgtcca tattggtttg ctgggcgtca aggcccgttt tggcgatcca taatgaactg
    34201 atcataaaat gtcctgtagt ggattagtga caaagtgcat atgtcttctt gccggatgcg
    34261 gcgtaaacgc cttatccggc ctacaagtgc tcgcaaaatc gataaattgc gattgccccc
    34321 atagacccga taaacttgcg catcgggcaa cttatacttg tcatgtatcc tttcaattaa
    34381 ctcatcgaca gcagttggtt ggcacggcct gcgttatcat cgacgctgct gatcaccttc
    34441 atctgcattt caaaacgccg cgcgctggca atcatgtcgc tcattgccgc aacggcattg
    34501 acgttactgc cttccagaac ccccgacatc acacgcaagg ttggatctgc ctgcagtacc
    34561 ggcccacgcg tggcctgggt ttctgcgctt aaacgaaaaa tgccgtcgtc accgcgctgc
    34621 acttcgctgc ccgtggcttt caccagttta agacgcccta ctggcgcaac cgtatttgcc
    34681 ggatcgcccg gattgagcgc cgagattgtg ccatcggcag caatagtgat ttccgcccct
    34741 tccggcacag caattggccc agcctcgcct atcaccggat gcccctgaat tgtcagttgc
    34801 ccggtgggat caacctgaat gctgccatta cgcgtatacc cttcgctgcc gtcagcggtc
    34861 tgcacggcca gccagccatc ctgctgcaac gcgacgtcca gcgggcgcga ggtgtaatcc
    34921 attttgccgg gcgtcatatc tgcgcccggc gttgacgccg tgaccaacgt gcgcgtgggc
    34981 agagaaagcc cttccactgg caccgcgcgt aaagcattca actgcgcgcg aaaaccgggc
    35041 gttgaggcat tggccagatt actggcggtt accgcctgtt gattcagtgt ctggctggct
    35101 gctcccatcg cggtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa
    35161 gttaaccagc gtgttgagga tctggtcctg ggttttgatg gtctgggcgt tagactgata
    35221 gttacgctgg gcaacgatca tattgaccag ttctttactg agatcgacgt tggacgcttc
    35281 cagcgcaccg ttggtcaggg tgccaaagtt tcccgtcccg gctgtcccca acagcgccac
    35341 gccagaagat tgcgtcgcag accagacgtt gtcgccttcg gatgccagac cttcgttgtt
    35401 ggcaaagttc gccagtacaa tctgccccag cagttgggtt tgttcgttgg aatagttgcc
    35461 gacaaccgta ccgtcatcat tgatttgata actcaccaga tcgcccggtt tgtagccgtt
    35521 ctgggtggtt gccacaatat tgttagcgcc ggtattttgc tgcatggagt tgaggaagct
    35581 cagactaaac gtggcgggtt ctgcaccgtt aattgcgccg gttgcgatat tattcgccat
    35641 cgcaccatcc actaatgtgc cattagcatt aaattccagt gttgtcgctg tcttcgcaat
    35701 gctgtttgga tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac
    35761 aaagtagacg ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt
    35821 tttgttatag ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcact
    35881 ggaattcagg ttgatctgca tcgatgccgt ggtggtagtt ttcgctgcca tcagggtatt
    35941 cgggatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttgc
    36001 cgggtaaccc gtcagctgta aaccttgcat attcaccagg ttacggtttt catccagctt
    36061 aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc
    36121 gttctggctg atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa
    36181 gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    36241 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    36301 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    36361 gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtggtggt
    36421 gccgtaagtg ccgagatcca gcgtattacc gctgttgccg cggatcacac cctgcaccag
    36481 agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    36541 attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac gtaccgtccc aggtgaaact
    36601 gtgaactccg gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc catttttatc
    36661 ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt
    36721 cgtggtcaca gccccttctt cactgccggt tcccgcaaga acagtggtgc cggggatcat
    36781 cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga
    36841 aatagatccg agcgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccaa
    36901 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    36961 caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact
    37021 actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat
    37081 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg
    37141 agcacttcaa cattggcctg atagctgcgt gacgccgaca tggtgttaac catctctccg
    37201 acaacatcaa cgttcggcat ttttacgtag ccctttgcat ctgccagcgg attacccggt
    37261 tcataaacca gtttgtccgg ggcctgactt tctataacat cggcaacctt tacgccgcct
    37321 gtcgcagcac ctggtgcagc gttaacctgg aataccacct gttttgcccg atatggctgt
    37381 ccatcaggac cggtcacgct atcagcattt gccagattac tggccgccac gttcaggcgc
    37441 tgggactggg cagttaacgc cgacccggcg atatcaaaaa tattcagcag tgccatccgt
    37501 taatttccgc tctgtaaaac gttcatcatg cctttgattt gcccgctcaa cgcgctaagg
    37561 ctcatctggt attgcaggct gttatcggca aactgggtgc gttcgcgatc catatcgacg
    37621 gtattaccgt caagcgaagg ctggtccgga atacggtatt gcagttctgc ggtaggaggc
    37681 gtcagcgcct gcgccggaat gtgttgcgtt gaggtcatcg tcagtgcaac cacactggtt
    37741 gcatcccgtc cacgttgcat gactttttta agttcactgg caaaatcgat atcgcgcgcc
    37801 tgataaccag gggtatcggc attggcgatg tttgctgcca gcacttcctg acgctgggcg
    37861 cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct
    37921 cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc
    37981 ggaataaacg caaaatgggt cgctatttat gccgttgatg gtcattgcgg acaggtacaa
    38041 ttcacgttgt agaaatggct gggggcgaaa atgctgataa taaaacgtag cgtggcgatc
    38101 atcgcgatac tgttcagtcc gttaagtacg gcgagcaatc tcacatcgca attgcacaac
    38161 ttttttagcg cccaactcgc gggggtaagt gatgaggttc gtgtttctat tcgtacagcg
    38221 cccaatctac taccgccatg cgagcagcca ttgctttcga tgagcaataa ttcccgcctg
    38281 tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt acaggttaat
    38341 gtacaggcca caggaaatta tgtggttgcc gcgatgccca ttgcgcgggg aggaaagctg
    38401 gaagctggca atgtcaaact gaaacgcgga cggctggata ccctgccacc gcgtacggtg
    38461 ctggatatca atcaacttgt tgatgccatt agcctgcgcg atctatcacc cgatcaacct
    38521 atccagttaa cccagtttcg ccaggcatgg cgggtaaaag cgggacaacg cgtcaatgtg
    38581 atcgccagcg gtgatgggtt tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc
    38641 gtcgcacaga atgcgcgggt gcgcatggta tcgggacagg tagtcagcgg cgttgttgat
    38701 gcagatggga atattcttat aaacctgtaa gctgttaaag attacccgtc ccttgccgat
    38761 aaataagcaa cacatgataa aagcgccctc aatgaggaat aaaccatgag tattgatcgc
    38821 acttcgcctc tgaagcctgt aagcaccgtt caaccgcgcg aaaccactga cgcgccggta
    38881 acgaacaccc gggcggcaaa aacaacagcc tccaccagca ccagtgtgac gttaagcgac
    38941 gcgcaagcaa aactgatgca acccggcagc agtgatatca atcttgaacg tgtcgaagcg
    39001 ttaaaactgg cgattcgtaa cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg
    39061 ctgatcaacg aagcgcagca agacttgcag agtaactgat cgtatggcac gtcttgcaga
    39121 gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa acggtaatgg atcaagaaca
    39181 gcaacatctc tctatggggc agatcaacgg cagccagttg caatggatta cagaacaaaa
    39241 aagctcactg ctggcgacgc tggattacct cgaacagtta cgcaagaaag aacccaatac
    39301 agcaaatagc gttgatatta gtcaacgctg gcaggaaatt actgtgaaaa cgcagcaact
    39361 acgccaaatg aatcaacata acggctggtt actggaagga cagattgagc gcaatcaaca
    39421 ggcgctggaa atgttgaaac cgcatcagga accgacgcta tatggggcga acggtcagac
    39481 ctcaacaacc catcgcggcg gtaaaaagat ttcgatctga atattatctc cggcctgcac
    39541 tgcaggccgg aatgcattgt tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca
    39601 gtaccgccag tgcagcgaag tacgcggcaa tccccaccag cacgaccgcc attaaacgca
    39661 gtaaacgcca gggcatggta cccaatgacc actccggcat gatatgtaac atacctaaaa
    39721 gcacgccaga catcaccagt accgccacca ccagacgcaa cagaaacgcc atccagccgg
    39781 gttgcggggt aaagattttc tgcttacgca actgccagta aagcagcgaa gcattcagac
    39841 acgccgccag accaatagaa agtgacagcc cggcatgttt caacggacca ataaacgcca
    39901 ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat tttcactggc gttttaatgt
    39961 cctggcggga ataaaagcca ggagccaaca ctttcactac aatcaggcca atcaaaccca
    40021 ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc aaacgcggta aatttaccgt
    40081 actggaacag cgaaacggtc aacggaccgg aaagaatgcc caacgcaacc gcactcggca
    40141 gcgccaacag gaaacaaaga cgcaaccccc agtccatcaa acggttgtat tcatcatgat
    40201 tgccactggc aaaacttttc gacagcgacg gcagcaaaat ggtgccaagc gccacgccca
    40261 gcacaccgga cggaaactcc attaagcggt cggcgtaata catccaagac accgaaccgg
    40321 aagcaagaaa cgaggcaaaa atggtgttga tgattaagga gatctggctc acagagacgc
    40381 caaggatcgc cggtcccatc tgtttcacca cgcgcattgc tccggcatcg tggaagttaa
    40441 tgcgcggcag gaccagcatg ccgatcttct tcaggtgcgg tagctgatac accagctgca
    40501 ggacgccgcc gaccgtaaca gcccacgcca gcgccagcac cggtgggtta aagtacggtg
    40561 cggcaaacag cgcgaaacca atcatgctga tgttaagcag tgttggagca aacgccggaa
    40621 tcgagaagcg gttccacgta ttcagaatcg ctcccaccag cgacgccagg gagatcagca
    40681 agatataggg aaaggtaatc tttagtagct ggctggtcag ggcaaatttg tcagctgtgt
    40741 cagcgaagcc tggcgcggtc accatgatca cccacggtgc ggcgagcatg ccagcgaccg
    40801 tcacaaccgc cagcgcaagt gtcagcaggc cagaaacata agagacaaag acccgcgtgg
    40861 cgtcttcacc ctgcttactt ttatattccg ccagaatcgg tacaaatgcc tgggaaaatg
    40921 ccccttcggc aaagatacgg cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt
    40981 cggttgccat ccctgcgcca aagattctgg cgacaattgc gtctcgtgcg aagccaagca
    41041 cacgcgaaaa catggtcatc gagctgacgg cggccagcga ttttaataaa ttcatcggtg
    41101 ttctaatcca gacccacggg caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt
    41161 ctacctggat tatggtgaat tgctaccgcc agatgttaca gggtttattc actcatcgca
    41221 tcgcgccaga tcttgtcaac gatacgttgc gccagcacgg cctgttcgcc ggcggtttgc
    41281 ggaactgtct ggttttgcac acattcaatg aagtgccgcg cacagccgac aaacccacgt
    41341 tgctcaagcg tactctgcca accaggaatc ggtttatgca ctacgccctg cccgcgctcc
    41401 tcacgccatt cgcgcatatc cgtaatgtcg atgagcgcac cgtcagtcac ggcctgcacg
    41461 gtttcacgct gacttccggc acggcgatgc atacaggtgg tgatctgcaa aggaccagcc
    41521 gaaaaatggt gctcggcaaa cagcatttcg ccagcgtcgt tagtcagtag cgtaccgcca
    41581 tccagagagg ctttgccgcc cgacaaccac agcgcggtat ccaccacatg cagataatca
    41641 tccagcaacg tgaaataaag atcgtgtggc ccgacgctat tgctacgatg tttatccatt
    41701 cttagcgagg ctgcggtggc gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga
    41761 cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg ccagttccac cagccgttca
    41821 gcatcgcgca gattttctgc cagcggttta tcgacacaga catgtacccc cgcattgagt
    41881 aacgtactga ccacgtcaaa gtggctggcg gtgctggaat gcacaaaaac cgcatcgcaa
    41941 ctggcggcaa ggctggataa cgaatcggca taaggaatgc gccagctttc acaaattggc
    42001 agggctttcg cgcgcgtagg cgaccaggct ccttgtaacg tccagtcaga cgctgccgcc
    42061 agcaccggta accacgcttt ttgcgcaatg ccacctaatc ccactacgcc gatacgtaat
    42121 tttttcactt taatctccca gatgggccag caacgaatca agacgctgtt tcagttctgc
    42181 cacttcgatt tccagggctt cgacgcgggc ctgtaaatca ccgtcaaccg cgttcgacat
    42241 atccgtcacc gccggctgat cttcaacctc accactgaaa agatgcatgt agcggttttc
    42301 gcgtttaccc ggttcgcggg ccagacgcac cacaaaagga ccatcttcgc gatttgccag
    42361 ttgttccagc gtcgactcca cttccgccat atcgctgaat tcatacattc gcgcggcgcg
    42421 gctgcgcagt tcacctggcg tctgggcacc acgcaataac aacgtggtga ttaacgccac
    42481 ttccgctgcg ctcagtttca gatcgccaaa ttctgaatta caaaaacgtt gctcatattt
    42541 ggtgacccga ttaccaaaac cgctcactgt gcgtagataa tgacgtttga ccagattatc
    42601 cagctgttcc tgcacttcgg attcactcag attcatgacc ggttcacggt tcgttttctg
    42661 attacaggcc gtgactacac cattgactga gagcgggtat tgctccggcg tggtcacctg
    42721 cttttccagc aagcaaccaa tcacgcgagc ttcaagggca gtaagttgat atttcatgag
    42781 attctcctta gcggccgggc gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc
    42841 gccattgtcc atcaatcaac agatagtctt tcgcatagcc ttctttttca aaacccagtc
    42901 gcgccagtaa atcaccgctg cgtttattgt gcggcatata attagccata atgcgatgaa
    42961 tatgttgggt gcgctgcata taacgaatgg ctgcggtcag ggcttcaaac atgagtcctt
    43021 tgccctgcca tttttgccca atcgaataac cgagatagca ggcatgaaaa gagccacgaa
    43081 caacattgga aaaattggca acgccaataa tctctttttc gtccgggtcg aataagccaa
    43141 agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat ccccagcctg gcctgccagc
    43201 ctgatggata acagtggctt tcgtcgcgca ctggctccca gggcttgagg aaatggcgat
    43261 tctctgcgta ataatccgca agacgccagg catcacgatc atgcaccaga cgcacgacca
    43321 gtcggtctgt ggttaagcgc acttttggca cgttactgcg atagccaaac attctatacc
    43381 tactccttcc cgtaacgtct ttaatacgct gtaagtatta ctatacctgc gcaaccagac
    43441 cctgtgaaaa gtccaaaatg ccattttctg cattattcac atatttgata aaaactctct
    43501 atcaaagcga cgttttgata aaaaaatatt gtccccgaag tgatgggaaa agtcttagcg
    43561 gcaatgcaga atagaaagtg ctcatctttt atttcccggg aggggaaatg tcccgcgtgt
    43621 cgcaggcgag gaacctgggt aaatatttcc tgctcatcga taatatgctg gtcgtgctgg
    43681 ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg
    43741 ccgtcatggt cggtattgct ctcggtctac gccaatttat tcagcaaggt ctgggtattt
    43801 tcggcggcgc aattgccgac cgctttggtg ccaaaccgat gattgttacc ggtatgctga
    43861 tgcgtgccgc cggattcgcc acaatgggta tcgcccacga accgtggcta ttgtggtttt
    43921 catgcctgct ttcgggactc ggcggcacgt tgtttgatcc gccgcgttcg gcgctggtgg
    43981 tgaaactaat ccgtccacaa caacgtggtc gttttttctc gctgttgatg atgcaggaca
    44041 gtgccggtgc ggtcattggc gcattgttgg ggagctggct gttgcaatac gactttcgcc
    44101 tggtctgcgc cacaggggcc gttttgtttg tactgtgtgc agcgttcaat gcgtggttat
    44161 taccggcatg gaaactctcc accgtacgca cgcccgttcg cgaaggcatg acccgcgtga
    44221 tgcgcgacaa gcgttttgtc acctatgtcc tgacgctggc gggttactac atgctggctg
    44281 ttcaagtgat gctgatgctg ccaattatgg tcaacgacgt ggcgggcgcg ccctctgccg
    44341 ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac gttgctctat cctatcgccc
    44401 gctggagtga aaagcatttt cgcctggaac accggttgat ggctgggctg ttgataatgt
    44461 cattaagcat gatgccggtg ggcatggtca gcggcctgca acaacttttc accctgattt
    44521 gtctgtttta tatcgggtcg atcattgccg agcctgcgcg tgaaacctta agtgcttcgc
    44581 tggcggacgc aagagctcgc ggcagctata tggggtttag ccgtctgggt ctggcgattg
    44641 gtggcgctat tggttatatc ggtggcggct ggctgtttga cctgggcaaa tcggcgcacc
    44701 agccagagct tccgtggatg atgctgggaa ttattggcat cttcactttc cttgcgctgg
    44761 gttggcagtt tagtcaaaaa cgcaccgcgc gtcgtttgct ggaacgcgac gcctgatttg
    44821 atactccccc ctgactcctc catactgaaa gtgaactgac agccaaatgg aggagtcacg
    44881 tgaagctata catttacgat cactgccctt actgcctcaa agcccgcatg attttcggcc
    44941 tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga cgacgcagaa acacccaccc
    45001 ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga tgacagccgc tatatgccag
    45061 aaagcatgga tatcgttcac tatgtcgata aactcgacgg caaaccgtta ctgaccggca
    45121 aacgttcccc tgccattgaa gagtggctgc gcaaagtcaa tggctacgcc aacaaactgc
    45181 tgttgccgcg ttttgccaaa tcggcatttg atgagttttc tactcccgcc gcgcgcaaat
    45241 atttcgtcga caagaaagag gccagcgcgg gtaattttgc cgacctgctg gcccactctg
    45301 acggtctgat taagaatatc agcgatgatt tgcgtgcgct ggacaaactg atcgtcaaac
    45361 cgaacgccgt gaatggcgaa ctttcggaag atgatattca gctatttccg ctactgcgta
    45421 atctgacgct ggtagccgga atcaactggc caagccgcgt tgctgattac cgcgataata
    45481 tggcgaaaca gacacaaatc aatttgttat catcaatggc gatttaatcc tgacccgccg
    45541 cggcagcctg tattcgcggg ttgccgccgt tttcagtttt ttctgctggc gagtttatcg
    45601 ctggtgacac atgctgcgtt gtgtcaggat attgacgcag atttcatgtc gaggactacc
    45661 atgaacaagt ttttatttgc cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat
    45721 caactcaccc aatacaccat caccgaacaa gaaattaacc agtcgcttgc gaaacataat
    45781 aatttctcaa aagatatcgg tttacccggc gtggctgacg cccatattgt tctgacaaac
    45841 ttgaccagcc aaattggtcg cgaagagccg aataaggtta ccctaaccgg agacgccaat
    45901 ctggacatga actccctgtt cggtagtcag aaagcgacca tgaaactgaa ggtgaaagcg
    45961 ctgcccgtgt ttgataaaga aaaaggtgcg atcttcctga aagagatgga agtggtcgat
    46021 gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa cgttgcttcc ctatttgaac
    46081 caggcattac gcaattactt taaccagcaa cctgcttacg tcctgcgcga agatggcagc
    46141 cagggcgaag caatggcgaa aaaactggcg aaaggcattg aagtgaagcc aggcgaaatt
    46201 gtcattccat ttactgatta atcacgaggg cgcatttgcg ccctttattt ttcgtgcaaa
    46261 ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag
    46321 agattaatga ctgcaccatc ccaggtatta aagatccgcc gcccagacga ctggcacctt
    46381 cacctccgcg atggcgacat gttaaaaact gtcgtgccgt ataccagcga aatttatgga
    46441 cgggctatcg taatgcccaa tctggctccg cccgtgacca ccgttgaggc tgccgtggcg
    46501 tatcgccaac gtattcttga cgccgtacct gccgggcacg acttcacccc attgatgacc
    46561 tgttatttaa cagattcgct ggatcctaat gagctggagc gcggatttaa cgaaggcgtg
    46621 ttcaccgctg caaaacttta cccggcaaac gcaaccacta actccagcca cggcgtgacg
    46681 tcaattgacg caatcatgcc ggtacttgag cgcatggaaa aaatcggtat gccgctactg
    46741 gtgcatggtg aagtgacaca tgcagatatc gacatttttg atcgtgaagc gcgctttata
    46801 gaaagcgtga tggaaccgct gcgccagcgc ctgactgcac tgaaagtcgt ttttgaacac
    46861 atcaccacca aagacgcagc cgactatgtc cgtgacggaa atgaacgact ggctgctacc
    46921 atcactccgc aacatctgat gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg
    46981 cacctgtatt gtctacccat cctcaaacgc aatattcacc aacaggcact gcgtgaactg
    47041 gtcgccagcg gttttaatcg agtattcctc ggtacggatt ctgcgccaca tgcacgtcat
    47101 cgcaaagaga gcacctgcgg ctgcgcgggc tgcttcaacg ccccaaccgc gctgggaagt
    47161 tacgccaccg tctttgaaga gatgaatgct ctgcagcact ttgaagcatt ctgttctgta
    47221 aacggcccgc agttctatgg gttaccggtc aacgacacat tcatcgaact ggtacgtgaa
    47281 gagcaacagg ttgctgaaag catcgcactg actgatgaca ccctggtgcc attcctcgcc
    47341 ggggaaacgg tacgctggtc cgttaaacaa taaaaatcgt agcttcctgt tgtcattagg
    47401 ttattttacc tgtataaata accagtatat tcaacagggg gctattatgc gaattgaagt
    47461 caccatagcg aaaacttctc cattgccagc tggggctatt gacgccctgg ctggcgaact
    47521 ttcccgccgt attcagtatg cgtttcctga taatgaaggc cacgtatcag tacgttatgc
    47581 cgcagcgaat aatttatcgg ttattggcgc gacaaaagaa gataaacagc gcattagcga
    47641 aattctccag gaaacgtggg aaagcgccga tgactggttt gtcagcgaat aatatgcagt
    47701 gatttttgtt tttgccgggt cgccccggct tttttatttt acttacgaat tttgagtaca
    47761 aaatttctac acttcctcaa aataattgca caatatgcta aaaaaatatg aatgacatgc
    47821 tgttttattg ttcatataaa tcttaaaagc agaaatttat ctgttccccc ctttaaaaat
    47881 tcggtgaata cccttactta ttggtatact gaagttgctt cagataaaaa cgcattgaac
    47941 ctcgaataac gttgtctagt aacacgaatt agggggccat gatggaaaaa aataatgaag
    48001 tcattcagac tcatccgctc gtagggtggg acatcagcac cgttgatagc tatgatgcgc
    48061 tgatgttgcg tttgcactac cagaccccaa ataagtccga gcaggaaggg actgaagttg
    48121 gtcagacgct ctggttaacc actgatgttg ccagacaatt tatttcgata ttagaagcag
    48181 gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga gtatcggcgt cattaacatg
    48241 acatgaccat ccacccaaaa ggcacctcac cggtgccttt accatttctg ggttgtatat
    48301 ctgcattcag ttaattactc tgcttaaacc atgagagcag agagaaaaaa atgaaatacg
    48361 atctcatcat tattggcagc ggttccgtag gcgctgccgc cgggtattat gcaacccgcg
    48421 ccggtttaaa cgtgctaatg accgacgccc atatgccacc gcatcaacac ggcagccacc
    48481 acggcgatac gcgattaatt cgccatgctt atggtgaagg cgaaaagtat gtcccactgg
    48541 tactccgcgc gcaaacactg tgggatgagc tctcccgcca caacgaagat gatcccattt
    48601 ttgtacgctc tggtgtcatt aacctcggcc cggctgactc cgcatttctc gccaacgtcg
    48661 cccacagcgc cgaacagtgg caactcaacg tcgaaaagct cgacgcgcaa gggattatgg
    48721 cccgctggcc agaaatacgc gtcccagaca actacatcgg cttatttgag actgattccg
    48781 gttttttgcg cagcgaactg gcgattaaaa cctggatcca actggcgaag gaagcgggct
    48841 gtgcgcagct gttcaactgc ccggtcaccg caattcgtca tgacgatgat ggcgtaacta
    48901 ttgaaacggc tgacggagag tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg
    48961 taaaagacct gctcccggag ctgcctgtcc agcccgtacg caaagtattt gcctggtatc
    49021 aggccgatgg tcgctatagc gtgaagaata aattcccggc gtttaccggt gaactgccca
    49081 atggcgatca atattatggt tttccggcag aaaacgacgc gttgaagatt ggcaaacata
    49141 acggaggcca ggttatccat tcagcggatg aacgtgttcc gtttgcggaa gtggtcagcg
    49201 atggttcgga agccttcccg ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt
    49261 acggcgctgc ctgcacctat gataattcgc ctgacgaaga ttttattatc gataccctac
    49321 ccggccacga taatacactg ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt
    49381 cagttttagg ggaaatagct gccgattttg cgcaagacaa aaaaagcgat tttgatttga
    49441 cgccattcag gctttcccgc ttccaataat catcattacg gcctccagaa tgaggccgtt
    49501 tttacaggag tatttaatgc gtcggctgtt gcactatctc atcaataata ttcgcgagca
    49561 tctgatgctt tatctttttc tctggggatt gttggcaatt atggatctga tctatgtgtt
    49621 ttatttttag ttttccgcac tgacataaac tgacaaatat ttaatctccc tttcatttag
    49681 cgtgaaaaat tgaatgagat tattcctgct tctacagcac tcaccagaca tctctgatgc
    49741 tgaacccact aaacttatga agattctttc attattaatt caaaattatt gaaataaaaa
    49801 taaatatcat tcaatttgtt atttttgtgt tgcaattcat tctccgacac gccattttct
    49861 ttctctcgca aattcgtttt ggatgcactt atgtcattca caaatacccc tgaacgctat
    49921 ggtgttatat ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct
    49981 ttaggtctgt ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa
    50041 ctgcataaaa gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt
    50101 gtcatatctc caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgcg
    50161 aaagctggac atctcgcatt gtacctattg ctgttcgcca tcggcatcag cggctatctg
    50221 atctcaactg ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc
    50281 cttgccgacg ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg
    50341 agcgtcgtag tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat
    50401 aaagacgaca ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa
    50461 atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg
    50521 gttgccgccg attacaaaat tgacaaagaa ggtcagcacg cctttgttaa cttccgcatc
    50581 cagcatcttg gctatagctg gttatatggc acctttaaag atttcgacgg tacttttacc
    50641 tttgatgaaa aaaatccagc tgccgataaa gtgaatgtga caattaacac caccagcgtc
    50701 gatactaatc acgccgaacg cgataaacat cttcgcagtg cggatttcct caataccgca
    50761 aaatatccac aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat
    50821 attaccggcg atctgacgct gaatggcgta accaaacccg tcacgctgga agcgaaatta
    50881 attggtcagg gcgacgaccc gtggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa
    50941 attaagctca aggacttcaa tatcaagaca gatttaggcc cagcttctca ggaagtggat
    51001 ctgattattt cagtggaagg cgtacagcag aagtaagagt gaaatgttgc cggatggcac
    51061 gcatccggca atgatattta ttctgttgga tcaggaatgc ccagtgttgt attcagacgt
    51121 ccacgagact tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtctctgt
    51181 tcttctggcg ataacgcgct ttcttcgcag caaatctcac tacaacaacc tttgtatttt
    51241 tccgcgcata ctggacactg aataaacagc aggtggcagc catcattttt acagttggta
    51301 tggctgtcgc acgacgcacc gcactggtgg caatgcgcga taatctcatc cgatatacgt
    51361 tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc
    51421 tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga tatgccagac tttgttgaat
    51481 ccgttatgtt tcatccaggc actggccttt tcacaacgaa taccgccggt gcagtacatg
    51541 acgatttttt tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg
    51601 aaggtatctg ccggaatttc gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg
    51661 ttacgcatgt cgataaacag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct
    51721 tgcagatact caccaacatt gctggcatca aagtgaggat cgtcaatacc gtcggcaaca
    51781 atgcgatcgc ggaccttcat gcgcagtacc cagaaggatt tcccgtcatc atccaacgcg
    51841 atattcaggc gtaaaccctc taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt
    51901 tcaacattgc tcgctggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact
    51961 cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag cgtcacgggt caccttagga
    52021 tcggcaatgt ggaaatactt ataaaacgaa atggtggttc gcggttcgct ctcagccaac
    52081 attttggctt ttaacgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt
    52141 tcctgcgaga tgggaaagta aaaatccgcg gcatgatata gcaattatcg acaattaaca
    52201 tccacacatt ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca
    52261 atttcgccca gtcttcagtc acaaattttg gttgcggacg aaaaaatgcg acaatacata
    52321 caattgcccg tataggttga aaaacaggat tgatatgacg aatctaccca agttctccac
    52381 cgcactgctt catccgcgtt attggttaac ctggttgggt attggcgtac tttggttggt
    52441 cgtgcaattg ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt
    52501 acgttttatg aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga
    52561 aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg
    52621 cctgatggaa accggcatgg cgtggttctg gccggaccgc cgaatcgccc gctggacgga
    52681 agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt
    52741 tggcatccat tttctgacac tggagctggg cgcgcggcag tttggtatgc aggaaccggg
    52801 gattggcgtt taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg
    52861 tttgcgctca aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct
    52921 gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt
    52981 tttcgtcccc ttatttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc
    53041 acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg
    53101 gtatcaattg attatgctgc cgccagagtg ttctccgcca ctggatgatg ccgaaactac
    53161 cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg atggcaccag agcagtatat
    53221 gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc
    53281 ccatgccgga tgcttcagaa tggcatccgg cattaccaca gcaaatcccc ctgatttagc
    53341 gataaaagct ctctggattg cgccccctgg aagtcgggcg cataattagt gtgcttatct
    53401 tttcttctta tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga
    53461 aaatgacacc cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct
    53521 taccggtgcc gccttcagtc tggtaatgcc cttcttaccc ctctacgttg agcagcttgg
    53581 cgttaccggt cactccgccc tgaatatgtg gtccggtatt gtcttcagca ttacattttt
    53641 attttcggcc atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact
    53701 catgctatta cgctctgccc tcggcatggg catcgtgatg gtgttgatgg ggctggcaca
    53761 aaatatctgg cagtttttga tcctgcgggc gcttcttggg ttacttggcg gatttgtccc
    53821 caatgctaat gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcgctggg
    53881 tacgctctcc acaggcggcg ttagtggtgc gttgctcggc ccaatggctg gcggcctgct
    53941 cgccgatagc tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg
    54001 ctttttcgtc accctgtttt gcatcagaga aaaattccag ccggtcagca aaaaagagat
    54061 gctgcacatg cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt
    54121 tgtcactacg ttaatcatcc aggtggcgac gggctcaatt gcccccattc tgacgctgta
    54181 tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc
    54241 ggtgccaggc gtggcggctc tgctaagtgc accacgactc ggcaaacttg gcgatcgaat
    54301 cggacccgaa aagatcctga ttacagcgct gatcttttct gtactgctgt tgatcccaat
    54361 gtcttacgtt cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga
    54421 tggtgcacta ctccccgccg tacagacact gttggtttac aactcgagca accagatcgc
    54481 cgggcgtatc ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt
    54541 gatgggagca gcgatttcag cgaactacgg tttcagagcg gtatttctcg tcaccgctgg
    54601 cgtagtgtta ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca
    54661 ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt
    54721 ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag
    54781 aagccattga cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag
    54841 atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt
    54901 ggcaagttga gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg
    54961 gtgaagccgc gcaaagtgtt tttgatggcg actatgatga gatagagata cgccaggagt
    55021 ggcaggaaga gaatacatta catgaatggg acgaggggga atttcagctt gagccaccgc
    55081 tggataccga ggaaggacgc gcagcagctg atgagtggga tgaacgttaa tcactcatac
    55141 gggccatgat gaatgtcgat cggcagcagt aaagtatcaa aaaccaggga gaatggcaga
    55201 tcaaggattg tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa
    55261 tattggttgc catgcccctg ccccggtata gtgcgactaa taatgctgcc acagccactc
    55321 agcaaggtca ccatgatgct caccacaatt aaacgcattt gatacctctt ccgtaaaaaa
    55381 ataccggcat aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac
    55441 gccttaccca acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg
    55501 ataagcgtag cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa
    55561 tgccagtgga ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg
    55621 ggaattccag acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag
    55681 gcgacgatcg cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc
    55741 acgggcgatt tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag
    55801 agcgttaaat gacgggttaa acaccgcgtg cataaaacca tcatcaaggg agcgttgacg
    55861 attcatctca aggaagcgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg
    55921 gatcaggaac agtttccagc gtttggttcg cagaccaacg gtggcacggc tggaaatcac
    55981 cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg
    56041 cagatccaac cacgccatac caacagccca cactaaccct aacagcagct gtgagccgtg
    56101 gcgtttgaac gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac
    56161 cacttcccag ccaaggaacg cgctgacgac gaagaccgta tggaacagca tgcgtaccgg
    56221 agccagcagc acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc
    56281 gccgtattct ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa
    56341 caacagcacc atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca
    56401 taccgggaac aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catggactac
    56461 ctgcaatgca gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat
    56521 cacgcccgtc aggaacaccg cacggtgaac cgggtgcata cccttcacca ggaacagacg
    56581 gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt
    56641 aggcggcaat tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc
    56701 acggcgcatc aacgccgctt ccacgaagtc atgtgacagg attgaaccgg caaaggaacc
    56761 ttcgcccggc agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc
    56821 gttatgtccc cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag
    56881 tggcccatac acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga
    56941 cgctttcggc gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag
    57001 cccacacaaa caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta
    57061 ctggctgccc cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcgacg
    57121 acggcggcgg cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca
    57181 ggctttttgc tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc
    57241 aaagtgtttg gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa
    57301 aacacggttc acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt
    57361 taatggttca tcgccaactg ttgacgctga tatactgtat ttatcgcgac caataagcag
    57421 ttgcaggaag cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc
    57481 aaagaggatc aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca
    57541 caaatcctga ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat
    57601 ggtcttcata taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta
    57661 acggcggatg gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa
    57721 acgagccaga tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac
    57781 cgggttggta cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt
    57841 cagctgatcg cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca
    57901 ggcttgttcc agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt
    57961 gcggtgttcg gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc
    58021 tttctcgctt gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag
    58081 gtaactggta gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca
    58141 tttcagtggt tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg
    58201 taaccgggtt atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt
    58261 gcgctgtgac cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca
    58321 caaaggcgat agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg
    58381 aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc
    58441 tgaaggtgat ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg
    58501 tccagtaagc gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa
    58561 cgctgccttt accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac
    58621 gatcatcgag atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt
    58681 gcgggttctc catcgagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc
    58741 agatccattc gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac
    58801 gatagttatt tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg
    58861 caacccccag tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg
    58921 tgtcacgccc tggcataacc acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca
    58981 atgcatagat ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga
    59041 agcgcggaaa ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa
    59101 ggccataaac ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga
    59161 tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaagccaa
    59221 ggtcttttac cgtatctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt
    59281 atttgattcg tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt
    59341 acataccctg atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt
    59401 tgtcatgatt aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg
    59461 gcaagttgct tttgggcgtc tcgtagcctt tcccggctaa agattgagct tgctttgcga
    59521 catcatcaat actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac
    59581 tcaaccaacg cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc
    59641 tttgtgtgct tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga
    59701 atcccggtat cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca
    59761 ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt
    59821 tatttatcaa ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc
    59881 ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg tgtcactatg gcaacaagcc
    59941 taaaggcata acccgacagg gataagacga aaaatccgag attagttaac catatatgaa
    60001 cccagtaccc gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt
    60061 agggatacct tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc
    60121 cgaatcatca ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt
    60181 atttttcgtt atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa
    60241 atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact
    60301 gacattaccg caatttatta tgctgcaata tgtcaaagga aaagcggaaa gttggcctgg
    60361 gctgtcattg tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt
    60421 tttactggtg ttagtggtca tgacgacgct gtgcgtatgg atatttaagc gcatcagaaa
    60481 taatttagaa aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat
    60541 ttttttatgc ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc
    60601 gcccattctg agtaatggca tgttcaattt tattgtcatg caaacgctgt tttatttgcc
    60661 gttctttatc ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac
    60721 gccgtctcgt ggctgtaccc ttgcagcagc attggcgttt gtcgcttatt tactcaacca
    60781 gcgctatggc agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct
    60841 cggtctgtgg atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc
    60901 agcgcgggtg acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt
    60961 aacgctgttt ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct
    61021 ctgtggcctg atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat
    61081 cccgttactg aagtttttgt tttctggtaa gccggttgtt aagcgtgaga acgataaagc
    61141 accagcccgt taagccacat ttacaataac cattccacgg gcaatatcga tgccagtctg
    61201 accataaccc gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt
    61261 gcatgacgat caacccagtt gatccgtccc cacctgtcca gacggagctg ccaagccgca
    61321 tcatactggc tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc
    61381 acgaagccca tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata
    61441 aacaccgttt taccatcgat gctaaaagtt ttagcatgca ggctggctcc ggaattaccg
    61501 gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat
    61561 tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca
    61621 acatcgttag cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc
    61681 cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg
    61741 cgttcactgg gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt
    61801 gccttgccct cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc
    61861 ggcaacgttc catcaaccag atgatttata aacggactgg attccatttg gcgtaagtaa
    61921 cgatgcgtca tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca
    61981 ccttccggga catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg
    62041 gcgaaatcat cggcaacgtc ctctaccacg ggtcctattg ccataacatc taaatccgaa
    62101 aaaagtggct cttcccctgc cccaaaatag gcatcaccaa tatttcgtcc tcccaccagg
    62161 gtaaccacgc catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag
    62221 tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact
    62281 tcaatgcggg gatggctgtc aagcaagcgt aaaatatcgt caagtccggg cgtattgttg
    62341 tcgtccagca acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac
    62401 agtaaccgac ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct
    62461 gccatttcgg ccagacgata ccgggctgca aacgcatcca gacttttctc cagaggaaaa
    62521 aggccacact gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc
    62581 aaatcattca tctccttgtt gagtaaggag tctttcgtag aggtgggcgt tttcttcatc
    62641 ataacagaca aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt
    62701 ttttactgcg atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc
    62761 aggaaaagcc actgacgtaa agctgtttgc cgccaccagt cgtaggctat tgagataggc
    62821 atcctgcaaa agctggtctt cattttgttc accaccacgc cagactggcc cgacggtgtg
    62881 cactacggct ttagcaggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca
    62941 atcgccctgc tgttgcctga cttttaaaca agcatccagc agggccggac ccgctgcgcg
    63001 atgaatggcc ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat
    63061 cacatcaacg gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac
    63121 gcctccttac accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt
    63181 ttctgctcag caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca
    63241 gctttttctg tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat
    63301 tgccgctcat taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctgtgggc
    63361 agttcatttc tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc
    63421 cataaaaatg gatcacgccc ccttgcacgg tgcttccagc aatggcaata aaaggaaaaa
    63481 acaaactgcc gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa
    63541 tgattagtca tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag
    63601 accattctct ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag
    63661 agggcggcca ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt
    63721 tcacccgatc gtccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag
    63781 caggcaatga aagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca
    63841 gggacaatat ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg
    63901 tatacacatc cccttgctgg gtcgtattaa aggttatctg actggaaagt gccgcaagga
    63961 gtaataacgt attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta
    64021 atacaaatga tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc
    64081 acgttgacgg aggagttaga tgcagtctgg tcaactgcag caccgttgcc accaccgaac
    64141 tgtttaaccg tcatttcaga atttttgccg ttccactgat caagagtagc gctgttaccg
    64201 aagccacgtt gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca
    64261 ttaccgccgc catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca
    64321 agtgcagagt taccgccacc gtactggtaa atgttcagct cagaatttgg gccgctatta
    64381 ttaccgccac caccgtggtt accgccgccg ccgtactgag gaacaacacc tgccagagcg
    64441 ctaccggaga atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc
    64501 catcggattg atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg
    64561 cgactgtctc tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt
    64621 attaccagaa cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact
    64681 ggcatcgttg gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc
    64741 aatctttgcc cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc
    64801 ctgccgtaac tgagcactat tattagtccc agcttgacca attatggctg cctgattaaa
    64861 tgaagactta ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc
    64921 tgctgcggct gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt
    64981 tttcatgttg tcaccctgga cctggtcgta catttaagaa attaaatcat ttcaacttgg
    65041 ttgttaacgc aacctgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa
    65101 atatttttaa taactcaccc gcgcgttgta tttttctttt ttagttcata ccaaagtatt
    65161 aaaacccacg aaaatacgct gttatctacg caaaaaaata ttttgttttc ttttaaatct
    65221 ccgttttccg ctatcaaaaa gcaccagaca gtcattcttc ttgcccgtcg ctgattgctg
    65281 cgatatgtct tgcgcacaag ccgtgacaaa cctcgtcttt ttgcagtaaa aaattgtcca
    65341 cggaggtgtg gagaaaaaac aagaacgttt t
//
LOCUS       DAZFZC010000016        64185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFZC010000016 DAZFZC010000000
VERSION     DAZFZC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.016"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..353
                     /gene="yoaC"
                     /locus_tag="ACHKXP_001194"
     CDS             54..353
                     /gene="yoaC"
                     /locus_tag="ACHKXP_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO7646250.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(473..652)
                     /gene="yoaH"
                     /locus_tag="ACHKXP_001195"
     CDS             complement(473..652)
                     /gene="yoaH"
                     /locus_tag="ACHKXP_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO7646251.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            726..2087
                     /gene="pabB"
                     /locus_tag="ACHKXP_001196"
     CDS             726..2087
                     /gene="pabB"
                     /locus_tag="ACHKXP_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO7646252.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLASDWQSNMTREQYGEKFRLVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIFCSAGGGIVADSQEEAEYQETFDKVNRILKQ
                     LEK"
     gene            2091..2669
                     /gene="nudL"
                     /locus_tag="ACHKXP_001197"
     CDS             2091..2669
                     /gene="nudL"
                     /locus_tag="ACHKXP_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO7646253.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASAIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2853..4217
                     /gene="sdaA"
                     /locus_tag="ACHKXP_001198"
     CDS             2853..4217
                     /gene="sdaA"
                     /locus_tag="ACHKXP_001198"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7646254.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4348..5946
                     /gene="pdeD"
                     /locus_tag="ACHKXP_001199"
     CDS             4348..5946
                     /gene="pdeD"
                     /locus_tag="ACHKXP_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7646255.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFVNHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5950..7512)
                     /gene="yoaE"
                     /locus_tag="ACHKXP_001200"
     CDS             complement(5950..7512)
                     /gene="yoaE"
                     /locus_tag="ACHKXP_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO7646256.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHS
                     LFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRR
                     ARGSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHA
                     LQQALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVR
                     IVKEQPAHDEDE"
     gene            complement(7500..7658)
                     /gene="yoaL"
                     /locus_tag="ACHKXP_001201"
     CDS             complement(7500..7658)
                     /gene="yoaL"
                     /locus_tag="ACHKXP_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO7646257.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7975..8946
                     /gene="manX"
                     /locus_tag="ACHKXP_001202"
     CDS             7975..8946
                     /gene="manX"
                     /locus_tag="ACHKXP_001202"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO7646258.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9009..9809
                     /gene="manY"
                     /locus_tag="ACHKXP_001203"
     CDS             9009..9809
                     /gene="manY"
                     /locus_tag="ACHKXP_001203"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO7646259.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9822..10673
                     /gene="manZ"
                     /locus_tag="ACHKXP_001204"
     CDS             9822..10673
                     /gene="manZ"
                     /locus_tag="ACHKXP_001204"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO7646260.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10728..11186
                     /gene="yobD"
                     /locus_tag="ACHKXP_001205"
     CDS             10728..11186
                     /gene="yobD"
                     /locus_tag="ACHKXP_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO7646261.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYNLLVSTQ"
     gene            11615..12181
                     /gene="mntP"
                     /locus_tag="ACHKXP_001206"
     CDS             11615..12181
                     /gene="mntP"
                     /locus_tag="ACHKXP_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO7646262.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12178..12987)
                     /gene="rlmA"
                     /locus_tag="ACHKXP_001207"
     CDS             complement(12178..12987)
                     /gene="rlmA"
                     /locus_tag="ACHKXP_001207"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO7646263.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRGRLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13153..13362)
                     /gene="cspE"
                     /locus_tag="ACHKXP_001208"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13153..13362)
                     /gene="cspE"
                     /locus_tag="ACHKXP_001208"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7646264.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13375..13518)
                     /gene="yobF"
                     /locus_tag="ACHKXP_001209"
     CDS             complement(13375..13518)
                     /gene="yobF"
                     /locus_tag="ACHKXP_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO7646265.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(14187..14474)
                     /gene="yebO"
                     /locus_tag="ACHKXP_001210"
     CDS             complement(14187..14474)
                     /gene="yebO"
                     /locus_tag="ACHKXP_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO7646266.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(14549..14692)
                     /gene="mgrB"
                     /locus_tag="ACHKXP_001211"
     CDS             complement(14549..14692)
                     /gene="mgrB"
                     /locus_tag="ACHKXP_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO7646267.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14851..15090
                     /gene="yobH"
                     /locus_tag="ACHKXP_001212"
     CDS             14851..15090
                     /gene="yobH"
                     /locus_tag="ACHKXP_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO7646268.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(15233..16024)
                     /gene="kdgR"
                     /locus_tag="ACHKXP_001213"
     CDS             complement(15233..16024)
                     /gene="kdgR"
                     /locus_tag="ACHKXP_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO7646269.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            16201..17560
                     /gene="yebQ"
                     /locus_tag="ACHKXP_001214"
                     /pseudo
     CDS             16201..17560
                     /gene="yebQ"
                     /locus_tag="ACHKXP_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(17606..18487)
                     /gene="htpX"
                     /locus_tag="ACHKXP_001215"
     CDS             complement(17606..18487)
                     /gene="htpX"
                     /locus_tag="ACHKXP_001215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO7646270.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(18679..20727)
                     /gene="prc"
                     /locus_tag="ACHKXP_001216"
     CDS             complement(18679..20727)
                     /gene="prc"
                     /locus_tag="ACHKXP_001216"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO7646271.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(20747..21445)
                     /gene="proQ"
                     /locus_tag="ACHKXP_001217"
     CDS             complement(20747..21445)
                     /gene="proQ"
                     /locus_tag="ACHKXP_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO7646272.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(21542..22039)
                     /gene="msrC"
                     /locus_tag="ACHKXP_001218"
     CDS             complement(21542..22039)
                     /gene="msrC"
                     /locus_tag="ACHKXP_001218"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO7646273.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            22169..23452
                     /gene="yebS"
                     /locus_tag="ACHKXP_001219"
     CDS             22169..23452
                     /gene="yebS"
                     /locus_tag="ACHKXP_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO7646274.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            23421..26054
                     /gene="yebT"
                     /locus_tag="ACHKXP_001220"
     CDS             23421..26054
                     /gene="yebT"
                     /locus_tag="ACHKXP_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO7646275.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKMGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            26134..27573
                     /gene="rsmF"
                     /locus_tag="ACHKXP_001221"
     CDS             26134..27573
                     /gene="rsmF"
                     /locus_tag="ACHKXP_001221"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO7646276.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGE
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPTYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            27691..27927
                     /gene="yebV"
                     /locus_tag="ACHKXP_001222"
     CDS             27691..27927
                     /gene="yebV"
                     /locus_tag="ACHKXP_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO7646277.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            28032..28223
                     /gene="yebW"
                     /locus_tag="ACHKXP_001223"
     CDS             28032..28223
                     /gene="yebW"
                     /locus_tag="ACHKXP_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO7646278.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLHSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(28224..28880)
                     /gene="pphA"
                     /locus_tag="ACHKXP_001224"
     CDS             complement(28224..28880)
                     /gene="pphA"
                     /locus_tag="ACHKXP_001224"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7646279.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALGKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKVQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(29276..29617)
                     /gene="yebY"
                     /locus_tag="ACHKXP_001225"
     CDS             complement(29276..29617)
                     /gene="yebY"
                     /locus_tag="ACHKXP_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO7646280.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(29630..30502)
                     /gene="copD"
                     /locus_tag="ACHKXP_001226"
     CDS             complement(29630..30502)
                     /gene="copD"
                     /locus_tag="ACHKXP_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO7646281.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPM
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(30506..30880)
                     /gene="yobA"
                     /locus_tag="ACHKXP_001227"
     CDS             complement(30506..30880)
                     /gene="yobA"
                     /locus_tag="ACHKXP_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO7646282.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLIHQYPAANAQVKAS
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDPKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            31019..31249
                     /gene="holE"
                     /locus_tag="ACHKXP_001228"
     CDS             31019..31249
                     /gene="holE"
                     /locus_tag="ACHKXP_001228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO7646283.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            31351..32007
                     /gene="yobB"
                     /locus_tag="ACHKXP_001229"
     CDS             31351..32007
                     /gene="yobB"
                     /locus_tag="ACHKXP_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO7646284.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            32031..32693
                     /gene="exoX"
                     /locus_tag="ACHKXP_001230"
     CDS             32031..32693
                     /gene="exoX"
                     /locus_tag="ACHKXP_001230"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO7646285.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(32690..34750)
                     /gene="ptrB"
                     /locus_tag="ACHKXP_001231"
     CDS             complement(32690..34750)
                     /gene="ptrB"
                     /locus_tag="ACHKXP_001231"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO7646286.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(34959..35618)
                     /gene="yebE"
                     /locus_tag="ACHKXP_001232"
     CDS             complement(34959..35618)
                     /gene="yebE"
                     /locus_tag="ACHKXP_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7646287.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(35945..36301)
                     /gene="yebF"
                     /locus_tag="ACHKXP_001233"
     CDS             complement(35945..36301)
                     /gene="yebF"
                     /locus_tag="ACHKXP_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO7646288.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(36368..36658)
                     /gene="yebG"
                     /locus_tag="ACHKXP_001234"
     CDS             complement(36368..36658)
                     /gene="yebG"
                     /locus_tag="ACHKXP_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO7646289.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            36792..37970
                     /gene="purT"
                     /locus_tag="ACHKXP_001235"
     CDS             36792..37970
                     /gene="purT"
                     /locus_tag="ACHKXP_001235"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO7646290.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(38026..38667)
                     /gene="kdgA"
                     /locus_tag="ACHKXP_001236"
                     /gene_synonym="eda"
     CDS             complement(38026..38667)
                     /gene="kdgA"
                     /locus_tag="ACHKXP_001236"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO7646291.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(38704..40515)
                     /gene="edd"
                     /locus_tag="ACHKXP_001237"
     CDS             complement(38704..40515)
                     /gene="edd"
                     /locus_tag="ACHKXP_001237"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO7646292.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(40750..42225)
                     /gene="zwf"
                     /locus_tag="ACHKXP_001238"
     CDS             complement(40750..42225)
                     /gene="zwf"
                     /locus_tag="ACHKXP_001238"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO7646293.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            42563..43432
                     /gene="hexR"
                     /locus_tag="ACHKXP_001239"
                     /gene_synonym="yebK"
     CDS             42563..43432
                     /gene="hexR"
                     /locus_tag="ACHKXP_001239"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO7646294.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            43560..45002
                     /gene="pyk"
                     /locus_tag="ACHKXP_001240"
     CDS             43560..45002
                     /gene="pyk"
                     /locus_tag="ACHKXP_001240"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO7646295.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(45134..46105)
                     /gene="lpxM"
                     /locus_tag="ACHKXP_001241"
                     /gene_synonym="msbB"
     CDS             complement(45134..46105)
                     /gene="lpxM"
                     /locus_tag="ACHKXP_001241"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7646296.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(46225..47547)
                     /gene="mepM"
                     /locus_tag="ACHKXP_001242"
     CDS             complement(46225..47547)
                     /gene="mepM"
                     /locus_tag="ACHKXP_001242"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO7646297.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(47563..48495)
                     /gene="znuA"
                     /locus_tag="ACHKXP_001243"
     CDS             complement(47563..48495)
                     /gene="znuA"
                     /locus_tag="ACHKXP_001243"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO7646298.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            48574..49329
                     /gene="znuC"
                     /locus_tag="ACHKXP_001244"
     CDS             48574..49329
                     /gene="znuC"
                     /locus_tag="ACHKXP_001244"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO7646299.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVHAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            49326..50111
                     /gene="znuB"
                     /locus_tag="ACHKXP_001245"
     CDS             49326..50111
                     /gene="znuB"
                     /locus_tag="ACHKXP_001245"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO7646300.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(50258..51268)
                     /gene="ruvB"
                     /locus_tag="ACHKXP_001246"
     CDS             complement(50258..51268)
                     /gene="ruvB"
                     /locus_tag="ACHKXP_001246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO7646301.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(51277..51888)
                     /gene="ruvA"
                     /locus_tag="ACHKXP_001247"
     CDS             complement(51277..51888)
                     /gene="ruvA"
                     /locus_tag="ACHKXP_001247"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO7646302.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(52027..52092)
                     /gene="yobI"
                     /locus_tag="ACHKXP_001248"
     CDS             complement(52027..52092)
                     /gene="yobI"
                     /locus_tag="ACHKXP_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO7646303.1"
                     /translation="MYIFITHFFTEYVILKYLLPK"
     gene            52163..52765
                     /gene="yebB"
                     /locus_tag="ACHKXP_001249"
     CDS             52163..52765
                     /gene="yebB"
                     /locus_tag="ACHKXP_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO7646304.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(52767..53288)
                     /gene="ruvC"
                     /locus_tag="ACHKXP_001250"
     CDS             complement(52767..53288)
                     /gene="ruvC"
                     /locus_tag="ACHKXP_001250"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO7646305.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(53323..54063)
                     /gene="yebC"
                     /locus_tag="ACHKXP_001251"
     CDS             complement(53323..54063)
                     /gene="yebC"
                     /locus_tag="ACHKXP_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7646306.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(54092..54544)
                     /gene="nudB"
                     /locus_tag="ACHKXP_001252"
     CDS             complement(54092..54544)
                     /gene="nudB"
                     /locus_tag="ACHKXP_001252"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO7646307.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(54662..56434)
                     /gene="aspS"
                     /locus_tag="ACHKXP_001253"
     CDS             complement(54662..56434)
                     /gene="aspS"
                     /locus_tag="ACHKXP_001253"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO7646308.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            56744..57310
                     /gene="yecD"
                     /locus_tag="ACHKXP_001254"
     CDS             56744..57310
                     /gene="yecD"
                     /locus_tag="ACHKXP_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7646309.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            57307..58125
                     /gene="yecE"
                     /locus_tag="ACHKXP_001255"
     CDS             57307..58125
                     /gene="yecE"
                     /locus_tag="ACHKXP_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO7646310.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            58178..58573
                     /gene="yecN"
                     /locus_tag="ACHKXP_001256"
     CDS             58178..58573
                     /gene="yecN"
                     /locus_tag="ACHKXP_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO7646311.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            58614..59357
                     /gene="cmoA"
                     /locus_tag="ACHKXP_001257"
     CDS             58614..59357
                     /gene="cmoA"
                     /locus_tag="ACHKXP_001257"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO7646312.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            59354..60325
                     /gene="cmoB"
                     /locus_tag="ACHKXP_001258"
     CDS             59354..60325
                     /gene="cmoB"
                     /locus_tag="ACHKXP_001258"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO7646313.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(60490..62919)
                     /gene="torZ"
                     /locus_tag="ACHKXP_001259"
     CDS             complement(60490..62919)
                     /gene="torZ"
                     /locus_tag="ACHKXP_001259"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO7646314.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(62944..64044)
                     /gene="torY"
                     /locus_tag="ACHKXP_001260"
     CDS             complement(62944..64044)
                     /gene="torY"
                     /locus_tag="ACHKXP_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO7646315.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQTATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSEKELDILTRYFQYNAKDITEKQ"
BASE COUNT        15411 a        16521 c        15779 g        16474 t
ORIGIN      
        1 ttaaggtcat atatagcctt atcgataaca tggttaattt taaggataag aatatgcctg
       61 ctgtaataga taaagccctg gatttcattg gtgccatgga tgtatccgcg ccaacaccaa
      121 gttcgatgaa tgaaagcacg gcgaagggaa tctttaaata tttaaaagaa ctgggagtac
      181 ctgcgagtgc cgctgatatt acggcgcgag ctgaccagga aggctggaac cctgggttca
      241 cggaaaaaat ggttggatgg gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa
      301 atcctgaata cttttcaaca tatatgcagg aagaactaaa agcactggtc tgagttaaat
      361 ttatatcagc ataaatgggt caggacgctt ttaatcacat ataaaaagcg tcctttttct
      421 cacaatcaaa taatcatcgg cttctataac gaaatcgata cggattagtg tgttactcgt
      481 cttcatcctc aaaacgcgcc acgattcgct cgccggaatg gttggcacgc agctcttctg
      541 ccaccagcgc gattgcctgg ccgctgctca tcccttgtgc catcagctcc tggatccgct
      601 cgacggcttt ttgctgctgt tcatgggtga gtgaaggtaa acctgcaaac attgttaact
      661 cctgctaaat tgttggcgct aattatttca tgctacccgg cacatagcca gtagagtcag
      721 gactgatgaa gacgttatct cccgctgtga ttactttacc ctggcgtcag gacgccgctg
      781 aattttattt ctcccgctta agccacctgc cgtgggcgat gcttttacac tccggctatg
      841 ccgatcatcc gtatagccgc tttgatattg tggtcgccga tccgatttgc actttaacca
      901 ctttcggtaa agaaaccgtt gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc
      961 cgctacaggt gctccagcag gtgctggatc gcgcagacat tcgcccaacg cataacgaag
     1021 atttgccatt tcagggcggc gcactggggt tgtttggcta cgatctgggc cgccgttttg
     1081 agtcactgcc agaaattgcg gaacaagata tcgttctgcc ggatatggca gtgggtatct
     1141 acgattgggc gctcattgtc gaccaccagc gtcatacagt ttctttgctg agtcataatg
     1201 atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt ctcgccgcag gaagatttca
     1261 cgctcgcttc cgactggcaa tccaatatga cccgcgagca gtacggcgaa aaatttcgcc
     1321 tggtacagga atatctgcac agcggtgatt gctatcaggt gaatctcgcc cagcgttttc
     1381 atgcgaccta ttctggcgat gaatggcagg cattccttca gcttaatcag gccaaccgcg
     1441 cgccatttag cgctttttta cgtcttgaac agggtgcaat tttaagcctt tcgccagagc
     1501 ggtttattct ttgtgataat agtgaaatcc agacccgccc gattaaaggc acgctaccac
     1561 gcctgcccga tcctcaggaa gatagcaaac aagcagagaa actggcgaac tcagcgaaag
     1621 atcgtgccga aaatctgatg attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg
     1681 tagccggttc ggtaaaagta ccagagctct tcgtggtgga acccttccct gccgtgcatc
     1741 atctggtcag cactataacg gcgcgactac cagaacagtt acacgccagc gatctgctgc
     1801 gcgcagcttt tcctggtggc tcaataaccg gggctccgaa agtacgggct atggaaatta
     1861 tcgacgaact ggaaccgcag cgacgcaatg cctggtgcgg cagcattggc tatttgagct
     1921 tttgcggcaa catggatacc agtattacta tccgcacgct gactgccatt aacggacaaa
     1981 ttttctgctc tgcgggcggt ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg
     2041 aaacttttga taaagttaat cgtatcctga agcaactgga gaagtaagac gtggaatacc
     2101 gtagcctgac gcttgatgat tttttatcgc gctttcaact tttgcgcccg caaattaacc
     2161 gggaaaccct aaatcatcgt caggctgctg tgttaatccc catcgtccgt cgaccgcaac
     2221 cggggttgtt gctgactcag cgttcgattc atctgcgtaa acacgctgga caagtggcat
     2281 tccctggagg tgcagtcgat gacacggacg catcagctat cgccgccgcg ctgcgcgaag
     2341 ctgaagaaga ggtcgctata ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg
     2401 tcgatagcgt cactggctac caggtaaccc cagtggtcgg cattatcccg cccgatctgc
     2461 cgtatcgcgc cagtgaagat gaagtctcgg cggtgtttga aatgccgctc gcccaggcat
     2521 tacatctggg tcgttatcac cctttagata tctaccgccg tggtgattca catcgggtat
     2581 ggctgtcctg gtacgaacag tattttgtat ggggaatgac cgcaggcata attcgtgagc
     2641 tggcgctgca aattggtgtg aaaccctgac tatacttatc tttacatcta caaaacacta
     2701 cttgagacaa tcatcgcaat attagttaaa tcgcggtttt tgattagttt aattcatgtg
     2761 aatagttaag ccagtcgccg cgttccctct tacactatgc gctgttatta gttcgttact
     2821 ggaagtccag tcaccttgtc aggagtatta tcgtgattag tctattcgac atgtttaagg
     2881 tggggattgg tccctcatct tcccataccg tagggcctat gaaggcaggt aaacagttcg
     2941 tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac tcgcgttgcc gtggacgttt
     3001 atggttcact gtcgctgacg ggtaaaggcc accacaccga tatcgccatt attatgggtc
     3061 ttgcaggtaa cgaacctgcc accgtggata tcgacagtat tcccggtttt attcgcgacg
     3121 tagaagagcg cgaacgtctg ctgctggcac agggacggca tgaagtggat ttcccgcgcg
     3181 acaacgggat gcgttttcat aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc
     3241 acgcctataa cggcgatgaa gtcgtctaca gcaaaactta ttattccatc ggcggcggtt
     3301 ttatcgtcga tgaagaacac tttggtcagg atgctgccaa cgaagtaagc gtgccgtatc
     3361 cgttcaaatc tgccaccgaa ctgctcgcgt actgtaatga aaccggctat tcgctgtctg
     3421 gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa agagatcgac gagtatttcg
     3481 cgcatgtctg gcaaaccatg caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac
     3541 tgccaggccc gctgcgcgtg ccacgtcgtg cgtctgccct gcgccggatg ctggtttcca
     3601 gcgataaact gtctaacgat ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg
     3661 cagttaacga agaaaacgcc gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct
     3721 gcggtatcgt tccggcagtg ctggcttact atgaccactt tattgaatcg gtcagcccgg
     3781 acatctatac ccgttacttt atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg
     3841 cctctatttc cggtgcggaa gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg
     3901 ctgctgcggg tcttgcagaa ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg
     3961 aaattggcat ggaacacaac cttggtttaa cctgcgaccc ggttgcaggg caggttcagg
     4021 tgccgtgcat tgagcgtaat gccattgcct ctgtgaaggc gattaacgcc gcgcggatgg
     4081 ctctgcgccg caccagtgca ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg
     4141 aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc acgcggtggt ctggcaatca
     4201 aagtccagtg tgactaatac ttcttactcg cccatctgca acggatgggc gaatttatac
     4261 ccgctttctc gtctgctgta atattcccca ctacacttcc actgttgcgt caggcgtttg
     4321 tcgccatacg cttacagggt ggcccgcatg caaaaagcac aacggatcat taaaacctat
     4381 cgtcgtaatc gaatgattgt ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg
     4441 agcgtgcgat ttatttcaca gcgtaactta aatcaacaac gggtagtaca attcgtcaat
     4501 cacgctgtag aggaattaga taaagtactg cttcccctac aggcaggtag cgaagtcttg
     4561 cttccgctga ttggtctgcc ctgctctgtc gcccatttgc cattacgtaa acaggcggca
     4621 aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg gcacacttta ttgctccagc
     4681 atttttggtt atcgcaatgt gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa
     4741 ccacttttac gcctgacgat cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa
     4801 tggacgcctg cagcgggcag tagcaatgct ggggtcatgg agatgattaa catcgactta
     4861 ctgacggcaa tgctccttga gccacaactg ccgcaaatca gtagcgccag cctgacggtg
     4921 gacaaacggc atttgctcta tggtaatggg ctggtagatt cccttccgca acctgaagac
     4981 aatgaaaact atcaggtttc ttcgcaacgc tttcctttta ccattaacgt taatggtccg
     5041 ggggctacgg cgctggcatg gcactatctt ccaacacaat taccgctggc ggtgctgcta
     5101 agtttactgg tgggctacat cgcctggctg gcgaccgctt accggatgag cttttcccgc
     5161 gaaatcaatc tgggcctggc gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat
     5221 gcgcgcagcc agcaatgtat tggtgtagag attttgctgc gctggaacaa tccgcgtcag
     5281 ggctggattt caccggatgt gtttattcct atcgcggaag aacatcattt aattgtgcca
     5341 ctgacccgct atgtgatggc agaaaccatt cgtcagcgcc atgttttccc gatgagtagt
     5401 cagtttcatg ttggcattaa cgtcgcaccc agccattttc gccgtggtgt gctgataaaa
     5461 gatctcaatc agtactggtt tagcgctcac ccgattcagc aactgatcct cgaaatcacc
     5521 gaacgcgatg ccttactgga tgttgattat cggattgccc gcgagctaca tcgtaaaaac
     5581 gtcaaactgg cgattgatga cttcggcacc ggcaacagtt cattttcctg gcttgaaaca
     5641 ttacgtcctg acgtgctgaa aattgataag tcatttaccg cagctatagg ttctgacgcg
     5701 gttaactcga cggtgaccga tatcatcatc gcgctggggc aaagactgaa tattgaactg
     5761 gtggcggagg gcgtggaaac gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat
     5821 attttgcaag ggtatttgta cgcacagccg atgccgctac gtgattttcc caaatggctg
     5881 gcgggcagcc aaccgccgcc cgcccggcat aatggacata tcacgcccat tatgccgtta
     5941 cgttaatgct tactcatctt catcgtgcgc cggttgctct ttaacaatgc gaaccagatc
     6001 aacacgataa tcattggctt caatgatggt gatatgcagt ggccctacat caatcacatc
     6061 gcccacacgg ggaatgtgac catttgccga gatcacgagg cccgcgaccg tcgcgatatc
     6121 atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc aaggcatgca aatctgtacc
     6181 gccttttacc agccagccgt caccatcagt aatgatttcc ggcgtttcgt cagcgtccgg
     6241 gaattcaccc gcaatggctt ccagcacatc cagcggcgtg accagacctt gtaccacacc
     6301 aaactcgttg gtcacgataa caaagctccc gcgagcacga cgcagcacgc ccaacaggtt
     6361 gatcggatcg agggtttccg ggacgataat cgccggagac gccgaagcaa tcgccgccac
     6421 atcaacgccc tcttccagcg ccaccagcag ttctttagca cgtacaatac cgatgatttc
     6481 atccagttca ccgcgacata ccgggaacag actgtgcggt gaagagagca gttgctcgcg
     6541 gatttcatcg accccgagat tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat
     6601 cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta atcatgtaac gttcttcttc
     6661 ggcaaatgca ccttccggga tcgggatcgg catcggcatc gggttatcgg catcgtgctg
     6721 aacattggcc tgacgtttcc cgcccatcaa acgcaggatg gcatcggcag tacgcgctcg
     6781 cagcggcaaa gtcgactggt ggcgaataaa gttgcgacgc gcaatctggt taaacacttc
     6841 gatgatgatc gagaagccaa tcgcggcata caggtaacct ttcggaatgt ggaaaccgaa
     6901 accttctgcc accagactca gaccaatcat taacaggaag ctcagacaga gcaccaccac
     6961 cgtggggtgc tggttaacga atcgcgtcag cggtttggat gccagcaaca taaccgccat
     7021 cgcaatcact accgccgcca tcatcaccgg cagatggtta accatcccta ctgcagtaat
     7081 taccgcatcc aacgagaaga cggcgtcaag gatgacaatc tgtgtgacga ccacccagaa
     7141 actggcgtag cctttaccgt ggccggaatc atgatcgcgg ttttccagcc gttcatgcag
     7201 ttcggttgtt gctttgaaca gcaagaatat ccccccgaac aacataatca ggtcgcgtcc
     7261 ggagaaggag aaatccatga cggtaaatag cggtttggtc agcgtgacca tccatgaaat
     7321 cagcgacagc agccccagac gcataatcag cgccagtgat aaccccagca aacgcgcttt
     7381 atcgcgttgt tttggcggca gtttgtcagc aagaatggcg atgaagacca ggttatcgat
     7441 acccagcaca atttcgagaa caacaagcgt gagtagcccc gcccaaattg aggggtccat
     7501 taagaattcc atgacaagct cctgcttaag gaatagctat tcgacgccag aaataatgca
     7561 ggcgtaacga caaaatgcaa acgaaaggtg cggcatagag tgccagaaag gcaggcgtta
     7621 aaaggcctga tgctgaaatg acgtcggtga cgatccatac tgcgggctac tgccctatac
     7681 tccatggttg ttaaacggga gttaaacata tcagagacgc ctctgatttg gcaaagattt
     7741 accttccttt gcaaacgaat gtgacaagga tattttacct ttcgaaattt ctgctaatcg
     7801 aaagttaaat tacggatctt catcacataa aataattttt tcgatatcta aaataaatcg
     7861 cgaaacgcag gggtttttgg ttgtagccct tatctgaatc gattcgattg tggacgacga
     7921 ttcaaaaata catctggcac gttgaggtgt taacgataat aaaggaggta gcaagtgacc
     7981 attgctattg ttataggcac acatggttgg gctgcagagc agttgcttaa aacggcagaa
     8041 atgctgttag gcgagcagga aaacgtcggc tggatcgatt tcgttccagg tgaaaatgcc
     8101 gaaacgctga ttgaaaagta caacgctcag ttggcaaaac tcgacaccac taaaggcgtg
     8161 ctgtttctcg ttgatacatg gggaggcagc ccgttcaatg ctgccagccg cattgtcgtc
     8221 gacaaagagc attatgaagt cattgcaggc gttaacattc caatgctcgt ggaaacgtta
     8281 atggctcgtg atgatgaccc aagctttgat gaactggttg cgctggcagt agaaacaggc
     8341 cgtgaaggcg tgaaagcact gaaagccaaa ccggttgaaa aagccgcgcc agcacccgct
     8401 gccgcagcac caaaagcggc tccaactccg gcaaaaccaa tggggccaaa cgactacatg
     8461 gttattggcc ttgcgcgtat cgacgaccgt ctgattcacg gtcaggtcgc cacccgctgg
     8521 accaaagaaa ccaatgtctc ccgtattatt gttgttagtg atgaagtggc tgcggatacc
     8581 gttcgtaaga cactgctcac ccaggttgca cctccgggcg taacagcaca cgtagttgat
     8641 gttgccaaaa tgattcgcgt ctacaacaac ccgaaatatg ctggcgaacg cgtaatgctg
     8701 ttatttacca acccaacaga tgtagagcgt ctcgttgaag gcggcgtgaa aatcacctct
     8761 gttaacgtcg gtggtatggc attccgtcag ggtaaaaccc aggtgaataa cgcggtttcg
     8821 gttgatgaaa aagatatcga ggcgttcaag aaactgaatg cgcgcggtat tgagctggaa
     8881 gtccgtaagg tttccaccga tccgaaactg aaaatgatgg atctgatcag caaaatcgat
     8941 aagtaacgta ttgtgttgat tatcactcag ttttcacact taagtcttac gtaaacagga
     9001 gaagtacaat ggagattacc actcttcaaa ttgtgctggt atttatcgta gcctgtatcg
     9061 caggtatggg atcaatcctc gatgaatttc agtttcaccg tccgctaatc gcgtgtaccc
     9121 tggtgggtat cgttcttggg gatatgaaaa ccggtattat tatcggtggt acgctggaaa
     9181 tgatcgcgct gggctggatg aacatcggtg ctgcagttgc gcctgacgcc gctctggctt
     9241 ctatcatttc taccattctg gttatcgcag gtcatcagag cattggtgca ggtatcgcac
     9301 tggcaatccc tctggccgct gcgggccagg tactgaccat catcgttcgt actattaccg
     9361 ttgctttcca gcacgctgcg gataaggctg ctgataacgg caacctgaca gcgatttcct
     9421 ggatccacgt ttcttctctg ttcctgcaag caatgcgtgt ggctattccg gccgtcatcg
     9481 ttgcgctgtc tgttggtacc agcgaagtac agaacatgct gaatgcgatt ccggaagtgg
     9541 tgaccaatgg tctaaatatc gccggtggca tgatcgtggt ggttggttat gcgatggtta
     9601 tcaacatgat gcgtgctggc tacctgatgc cgttcttcta cctcggcttc gtaaccgcag
     9661 cattcaccaa ctttaacctg gttgctctgg gtgtgattgg tactgttatg gcagtgctct
     9721 acatccaact tagcccgaaa tacaaccgcg tagccggtgc gcctgctcag gcagctggta
     9781 acaacgatct cgataacgaa ctggactaac aggtgagcga aatggttgat acaactcaaa
     9841 ctaccaccga gaaaaaactc actcaaagtg atattcgtgg cgtattcctg cgttctaacc
     9901 tcttccaggg ttcatggaac ttcgaacgta tgcaggcact gggtttctgc ttctctatgg
     9961 taccggcaat tcgtcgcctc taccctgaga acaacgaagc tcgtaaacaa gctattcgcc
    10021 gtcacctgga gttctttaac acccagccgt tcgtggctgc gccgattctc ggcgtaaccc
    10081 tggcgctgga agaacagcgt gctaatggcg cagagatcga cgacggtgct atcaacggta
    10141 tcaaagtcgg tttgatgggg ccgctggctg gtgtaggcga cccgatcttc tggggaaccg
    10201 tacgtccggt atttgcagca ctgggtgccg gtatcgcgat gagcggcagc ctgttaggtc
    10261 cgctgctgtt cttcatcctg tttaacctgg tgcgtctggc aacccgttac tacggcgtag
    10321 cgtatggtta ctccaaaggt atcgatatcg ttaaagatat gggtggtggc ttcctgcaaa
    10381 aactgacgga aggggcgtct atcctcggcc tgtttgtcat gggggcattg gttaacaagt
    10441 ggacacatgt caacatcccg ctggttgtct ctcgcattac tgaccagacg ggcaaagaac
    10501 acgttactac tgtccagact attctggacc agttaatgcc aggcctggta ccactgctgc
    10561 tgacctttgc ttgtatgtgg ctactgcgca aaaaagttaa cccgctgtgg atcatcgttg
    10621 gcttcttcgt catcggtatc gctggttacg cttgcggcct gctgggactg taagactgtt
    10681 gtacactacc ggggcctttt ggccccgttt ttttatctgg aggattaatg acaatcacgg
    10741 acctggtact gattcttttc atcgccgcac tcctggcctt cgcgatctac gatcagttca
    10801 tcatgccccg ccgtaacggc cccaccctgc tggcaattcc tttgctccgg cgtggtcgca
    10861 tcgatagcgt tatcttcgtc ggattgattg tcattcttat ctataacaac gtcacgaatc
    10921 atggtgcgtt aataacgaca tggttattaa gcgcactggc tctgatgggt ttttatatat
    10981 tctggatccg cgttccgaag atcatcttta aacaaaaagg ttttttcttc gccaatgtct
    11041 ggattgaata tagccgaatc aaagcgatga acttgtcgga agatggcgtg ttggtgatgc
    11101 aattagaaca gcgtcggctg ttaatccgcg ttcgaaatat cgacgatctg gaaaaaattt
    11161 ataatcttct cgtttcaact caataagtta tgaatttagc caaagctatg tttagtgtat
    11221 ttttaataat cagacatagc ttaggctata ttacctcttc ccttatttgt tatttatttt
    11281 aacgtttcat tgatatataa atctaaatga aaatcgttat caataaagca atgaaataat
    11341 atattccaac agttgtttta tattctcaaa atatgttaag gttgcgccct catttgggca
    11401 gtagccgatt tccagattcc ggaaatgtac gtgtcaacat actcgttgca aaacgtggca
    11461 cgtacggact gaatactttc agtcaggcga gaccatatgc acatcaatcg ctatgcctgc
    11521 atgaacgcga aaaccatcac ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga
    11581 tgtgttttat ggataccccg gtcaggacat tgtcatgaat atcactgcta ctgttcttct
    11641 tgcgtttggt atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg ccaccctcca
    11701 taaaccgaaa ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct
    11761 gacgccgctg atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa
    11821 ccactggatt gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt
    11881 tcgtggcgca gatgatgaag atgaagagcc gcgccgtcga cacggtttct ggctactggt
    11941 aaccaccgcg attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct
    12001 gcaggtcaac attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcaac
    12061 attagggatg atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct
    12121 cggcgggctg gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta
    12181 ataagaacgc tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc
    12241 tgccagtgtt tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa
    12301 tgcgacggct tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa
    12361 tgtaaaacct tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag
    12421 ccccttcagc tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg
    12481 cttcactact cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc
    12541 catactggta tcggaaaacg gcaaacggtg gctggaagcg acacaaaaag tgacctgcgg
    12601 atagcgtttc gccgccgctt ttatcgctac cttcgaaaca tccagaccaa acgtggtgat
    12661 ttcgggcaac gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag
    12721 caccgccgtg gccttatcat caagccgtcc cctcagttgg gcgacaattg catcacgcag
    12781 cggctgataa tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc
    12841 gcccggatca cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc
    12901 catatcaaac tgatgtcgct ggggacagat atagctgttt ttttcacgcg aaagaggctg
    12961 atggcaaagt ggacaggaaa aagacatgac aactccggca ggatactaaa ggccgcaagt
    13021 gtaacgcgaa tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt
    13081 aaaaagcctc gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat
    13141 cagtggattc gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg
    13201 aatttcgaac tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc
    13261 ggagaagtgt acgaacacgt ctttgctgcc atcagccgga gtaatgaagc caaaaccttt
    13321 agactcgttg aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat
    13381 tgttttcttc gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc
    13441 aatataaggc tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt
    13501 actgaaaatg ccacacataa acagaactgt acctcgttta acccgaaatc tgttatcaca
    13561 tacaacgtta attatggcaa gccattttta aacatgtctc gatcagacgc acaaatctcg
    13621 atacgtcttc cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt
    13681 gcttaacctt gccagttcag gcagatactt aaactggcgt attttctaac atagttcaat
    13741 aagttcagtt gttccaggcc agtgggagaa gttattacat agtgcgtgca atatcacatt
    13801 ttttgctatg caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg
    13861 gctatcaatt accctaaaga gaagtcaatc cccaaaggga ttgaaaattt aaaataagaa
    13921 aaattgatga atgagcaaaa aatcaagaga gaaacgtttc gctaataaat aataccacgc
    13981 ttaatcacaa atccgatgta aacatcctaa taaattaatg aggaaaatat ctttttctga
    14041 tatcccgtta cgcaaataaa taatatgata attatttgaa gtcagaccag agttttaatt
    14101 tgaagcagtt gcaccatggc agtgcgtata atcaggatga attgagtaac ccttcaacga
    14161 cggggttact caagattgag gaaggattat cgttcggcga ccagacgaat aatatcttcg
    14221 tcttcaaccg atttttgact ctcaacggtc tttttatctg ctttctcagg ttcgtttgct
    14281 tcgcacaaac gacgtaacag tgcattttga cgtttttgct gatccagcaa cgcctcaagc
    14341 agttcaatct gttcgttagt ccgagaactg gcacgattga taaaaaacca caagatgagc
    14401 ccgataagaa gaaccaccac cgatacaacc aaagacgcaa tgttcatcac gcctgaattt
    14461 acaacttcgt tcatttcacc acctctgagt agaggcgcta ttctaccact gctggagagg
    14521 aagaaaatct agtgctgaaa aaatgatatc accacgggat aaactggtta atggcacaaa
    14581 ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt gaatacctgc gcccaaagca
    14641 gcaagcaagc caacaccacg acaaccagaa cgacccatcg aaactttttc actccaatct
    14701 ccgcttcacc taccttatgt cattaaacta gcatgcgtta gttaaacagc gcctaactat
    14761 gtcgatattc gtgctttttc ggaatgagta gcttgcttca gcgcatattg ccgctacgat
    14821 taagcgaaca taaaaagaga agaggttgta atgcgattca tcattcgcac agttatgctg
    14881 atagcactgg tatggatagg attattactc agcggatatg gcgttttgat tggaagcaaa
    14941 gagaatgccg caggattagg tttgcaatgt acctatctga ctgccagagg caccagcacg
    15001 gtacaatact tgcacactaa aagtggtttc ttggggataa cagattgccc actgttacgt
    15061 aaaagcaata tcgtggtcga taatggttaa gggtaataaa taaccagatt ctcactcatg
    15121 agtaaaatat tcttatagca agcgtgttta atataacgca ccttcaactt tatttttctc
    15181 atgctaaata aagaacctgt ctgtttgata ttccgacagg ttcttactct catcagaacg
    15241 gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc tgcggtatgc aacattgcga
    15301 catattcctg taaacgctct tcagagaaac gcaacgtcgg gaaggagatg ctcaaaccgg
    15361 caatgaccac gccaaagcga tcaaataccg gtaccgcaat gcatcgcagc ccttcttcct
    15421 gctcttcgtt atcttcgcca tacccctgct cgcgcacctg gtccagaacg ggtaataacg
    15481 cttctgtact ggtgatggtc cgctcggtac tgcgtttata ctccacgccc tcaagaattt
    15541 gcttcacttc atcgcgatcg cgccatgcca gcagtacctt accaatcgcg gtgctgtaca
    15601 gcggattacg acgcccaatc cgtgaataca tgcgcaaatt gtacatagag tcaattttgt
    15661 gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg gatagtttct ttggtcaggc
    15721 gggagatctc acgcatctgg atatctgcgc tacgaattaa atcgacgttt tgtaacgcgc
    15781 gagcgcccag ttcaaacaat ttcagggtca gggaatattt ctccgactcc ccttcctgcg
    15841 ccacataacc taaggttttc atggtctgta aaaagcgata aacggtgctt tttgacatca
    15901 tgacgcgctg cgacagctcg gttatcccta tttcgcgctc ttcacccagc gcctgcaaaa
    15961 tgccaaaaac ttttagcacg gaagatacag aatcaggctg tttatccaga tctgcgttag
    16021 ccatttatca cctcattgcg agtgttttat aaaaatcaga actgtttttt attataattt
    16081 cgcaccaggg tggtcgcaat ccatcttttg ccggttagtt acaattctgc gacatccacc
    16141 gtgaatatca gtgctagaat catacccctg ttgattattc accaaagata taaaattcct
    16201 atgccaaaag ttcaggccga cggcctgcca ttgccccagc gatacggtgc gatattaacc
    16261 attgtgattg gcatttcgat ggctgtcctt gacggcgcaa tcgccaacgt cgccctgcca
    16321 acaatcgcca cggaccttca tgccacgcca gccagttcca tctgggtagt gaacgcctat
    16381 caaatcgcca ttgtcatctc cctgctctcg ttttcgtttc tgggcgatat gtttggctat
    16441 cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt cttcactgtt ctgcgccctt
    16501 tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac aaggtttcgg cggtgcagcg
    16561 ttgatgagcg ttaataccgc acttatccgc ctgatctatc cacaacgttt tctggggaga
    16621 gggatgggca taaactcgtt tattgttgcc gtctcttctg ctgccgggcc gacaattgct
    16681 gcagcaatcc tctccatcgc atcctggaaa tggttatttt taatcaacgt accgttaggt
    16741 attatcgccc tgcttctggc gatgcgtttt ctgccaccca atggttctcg cgccagtaaa
    16801 ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa ccttcggcct gcttatcact
    16861 gcgttgagtg gtttcgctca ggggcaatcg ctaacgttaa ttgctgcgga actggtggta
    16921 atggttgttg ttggtatttt ctttattcgc cgccagcttt ctcttcccgt accgctgcta
    16981 ccggtggatt tactgcgtat cccgctgttt tcactttcta tttgcacatc tgtttgctct
    17041 ttctgcgcac aaatgctggc aatggtttcc ctgccctttt acctgcaaac cgtgctcggg
    17101 cgtagtgaag tcgaaacagg tttacttctg acaccgtggc cgttagcaac gatggtgatg
    17161 gctccgctgg caggctattt gattgaacgc gtacatgcag gattgctggg tgctttaggg
    17221 ttgttcatca tggctgcggg gcttttttcc ctggttctgc tgcccgcgtc acctgcggat
    17281 atcaatatta tctggccgat gatcttatgt ggcgctggat ttggcttatt ccagtcaccc
    17341 aataaccaca ccattattac ctccgctccg cgcgaacgta gcggtggggc cagtggcatg
    17401 ttaggaacgg ctcgtctact gggtcagagt agtggcgctg atgctaaatc agttcggtga
    17461 taatggtacg cacgtttcgc tgatggctgc ggctattctg gcagtgattg ctgcctgtgt
    17521 gagtggttta cgtatcactc agccacgatc cagggcataa aaaaagcgcg tcgatcagga
    17581 cgcgcttttt agtatttact tcatattact tcaggtattc acccgtacgc agagcttcaa
    17641 ttcgtttatc cagcggcggg tgagtcatga acaactcact gagcgatttc gacttaccgt
    17701 taatgcagaa agccatcatg ctggttgctt cttgcggttc atagctggtt ttcaggcgct
    17761 gcagcgcggc aatcattttc tcgcgaccaa ccagttttgc cgaaccggca tcagcatgga
    17821 attcacgatg acgcgagaac cacatggtga taatgctcgc cagaatacca aacaccagtt
    17881 ccagaaccgt tgcaaccgca aagtagatca gcgggttgcc gttgctctct tcaccttcat
    17941 cacgatttcc gcccataaaa cccgcggcaa gctgcgccag aatacgggaa ataaagataa
    18001 cgaaggtgtt caccacgccc tgaatcagcg tcatggtaac catatcacca ttggcgatgt
    18061 ggctgatttc gtgagcaatt accgcctcgg cttcatccgg gctcatgttc tgcagcaaac
    18121 cggtgctgac agcaaccaga gaggcatcac ggcgcgcacc ggttgcaaaa gcgttgatgt
    18181 ccggcgcatg gtagatagcc acttgcggca tagcgatccc cgcctgacga gcctgggttg
    18241 ctacagtatt gaccagccaa cgttcccttt cgttacgcgg ttgctcgatc acttccccgc
    18301 caacagatcg taatgccatc catttggaca tcagaagcga aacgaaggaa ccaccaaaac
    18361 cgaacagcaa ggccatgatc atcagcccct gaacgctgct cgactgtatc cctgtcaggc
    18421 tcagtaccag cccgaaaacg accattacgg ccaggttcgt tagcaggaag agcgcgattc
    18481 gcatcataat tttcttttta cctcagttta acaaaacgca atatgcgata cccacatcgt
    18541 atgggttacg cgactatttt caagtctgga tagtgcgtaa gtcaccagaa agacacaact
    18601 ttacattttg tagcatctga tttacggcat cttgccgctg ttaaaaaatc aggcacaatt
    18661 tcttgtgcct gattgatatt acttgacggg agcgggttgt tccgcgggtc tggctttttc
    18721 aagcttcgcc agatcgagtg cgatattcac cgtctcatcc agataaggat ccggctcctg
    18781 gtaatctttc ggtagatcat ccagcttctt caactccggt ttaccttcgc gtttaaagcg
    18841 ttcgttcaaa cgcgccagac gcgtcgcatc atcttcatta ttctctttct cacgcacagc
    18901 gtagttcaga gaaacgatat tgcgcttgtc cttcatagcg ttgaagcgcg cgatatcctt
    18961 catgatgttc tggaactcag gatctttcgc gatacgcgca ttatgttcct tcagcagctc
    19021 cggttcaaag gcagttaaat ctcctgattt cacataagtc gcggcatcga tgctatccca
    19081 cggcagcgcg ttatcttcga atttctcacc cgtttccgtt tcttcattac ccgtcggcat
    19141 gatgatgtct ggcgttacgc ctttacgttg cgtactgccg ccgttaacgc gatagaattt
    19201 ctggatcgtg tactgcacag aacccagcgc tggccattca ggacgtaaca tctgatcgta
    19261 aatacggttc aatgaacggt attgctgaac ggtgccttta ccaaacgtcg gttcacccac
    19321 aaccagcgca cgaccgtaat cctgcattgc cgcggcaaag atttctgaag ccgaagcact
    19381 gaaacggtca accagtacca ccagcgggcc tttatagaac acctgcccgt cggtatcgct
    19441 gtcttcacga accttgccgt tgttatcgcg gacctgaaca atgggacccg caggaataaa
    19501 cagacctgag agcgataccg cttcggtcaa cgccccaccg ccattgctac gcaggtcgat
    19561 gatcacactg ctgacattct gtttttccag tttctgcagt tgaactttga catcgtctgt
    19621 caaacccaca tagaagcccg gaatatccag cacgccgact ttctctttac cgacggtctt
    19681 caccgacatt ttaaccgcgc ggtcttcgag acgaatacgt tcacgggtca atgttacagt
    19741 gcgggtcttg gtccctttac cagcaggtaa aatttccaga cgaactttac tgcccttcgg
    19801 ccctttaatt aaggcaacca catcatcaag acgccagcca atcacgtcaa ccatcggctt
    19861 gcctgtttga ccaacaccga caattttgtc accaacgctg atagctttac tcttcgctgc
    19921 cggaccacct gccaccatcg aattgataac ggtgtagtca tcatccattt gcagcactgc
    19981 gccaatacct tccagcgaca aactcatttc agtgttgaac tgttcggtat tacgcgggga
    20041 aagatagttg gtatgcgggt cgatttcacg cgcaaacgcc gtcattgcca gcgagaaaac
    20101 atcttcgctg ttggtttgcg ccagacgacg aatggcaaat ttgtagcggc gtgtcagggt
    20161 ttcacgaatt tctttatccg tttttcctgc cagcttcagg cttaactcgt cgaatttgac
    20221 tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc ggccagggcg ctttgctgcg
    20281 gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc ttttccagta ccgacaaagc
    20341 gtactggtaa cgctcaaaac ggcgcttttg cgccagattg tagagatcgt agaaaacgtc
    20401 gagtttgcct gaacgcagtt catcgcctaa ctcggttttc tttttcgcga actgttcaac
    20461 atcgcttgcc agcagcacgt tgtggctgta atcgagcaga ttcaggtagc ggtcaaagat
    20521 tttggccgaa aatgcctgat cgaggtcgaa ctggcgataa tgagaacggg tgaagcgcga
    20581 cgttacgcgc tcacttaccg tcgcatgctg cgtctcttcc tttaataccg gaatttgatc
    20641 agcacgcgtg atatcttcta cagcgaaggt ctggcctgct attgcaagca ggccagctaa
    20701 cgcggtaagc ctaaaaaaca tgttcatgcc tggcccggcc tccgtttcag aacaccaggt
    20761 gttctgcgcg cacaatcaaa gacatacccg aattcagctg gacgcggacg ccgtctttgg
    20821 tgatttctaa tacggtggca tccatcgcgt tttgacccgc tttcaccttc agggcttgtc
    20881 cgacagtcag agctgaaatg tcagaaaccg gggtgtgctg ttcttcgcga ggtgctttta
    20941 ctgtttttgg cgctttctct accggctttt gcgcacgagg tttacgttca gcgccttctt
    21001 tgcggcgtgg cgtggtcgga cgtggcttgc gttcgcggcg cggtgcgtct tctttctcac
    21061 cagcagctgc ggcagcttcg cgttttttcg cttgctgttc agcacgctgt gcctgaacac
    21121 gcgctttcgc ttcttcaagc tgcttgcgag catgctctac atgttgctcg tccagctcac
    21181 cgcatgggtt gccgtcaaga tcgacacgcg ttgcgccggg tttaacaccg taaagataac
    21241 gccagctcga agtgtagaga cgtaaagcgg atcgtaattg cgttttgctc aggttcattt
    21301 ccccggcaac acgatcgacc aaatcctgaa aaataccgat tttcagcgga cgcgcttcac
    21361 cttccgcact gaaacagtgg ggaaaacgtt cggccagaaa cgcgattact tctttactgc
    21421 tattcaactt aggttgattt tccatgaaat ttcctgatta caacggacgt agccaacaag
    21481 cgcaggcatg aacaggcgtc attataatga cgctaccagt aaatgctacg ttatccgttg
    21541 attatcctgc gacgctcgca aagaattttt tgtaatccgt cgttgcaagc actttttcaa
    21601 gctgtgccac aagctgacgt aagccttgct cgtcctcgtc tgtaaagcga ccgaagacgg
    21661 tactatcgat gtcaagaaca ccaataatct gatttttcac caccagcggc agaacaattt
    21721 cagaattact cgccgcatca caggcaatat gcccgtcaaa cgcgtgaaca tcctcgatac
    21781 gctgcacttg attgcgggca accgcagtgc cgcacacccc gcgcccgacg ggtatccgga
    21841 cacaggcaat tttgccctga aatggtccga gtaccagtgt atcgtcctca agcaaataaa
    21901 aacctgccca gtttacgtca gtgagacgct cgtataacaa cgcactggtg ttcgcaagcg
    21961 ttgccagaaa actggtttct cccgccatca gcgcgttaaa gtcgcgattt aaatccgcgt
    22021 aaaattctgt tttgttcatt atataatcac ttgtttgtct tacctggaac tgccagccta
    22081 ttaaaataag cattaaatgc gttaatgctc aagatcattc ccatcatggg ttaagattaa
    22141 tgttaattct tattacattt ggcacgtcat ggctcttaac acaccacaaa ttacgccgac
    22201 aaaaaagata acagtgaggg caatcggcga ggaactgccg cgtggtgatt accaacgttg
    22261 cccgcaatgt gacatgctgt ttagcctgcc cgagataaat tctcatcaga gtgcctattg
    22321 tccgcgctgt caggcaaaaa ttcgcgacgg gcgcgactgg tcgctaacgc gcctggcggc
    22381 aatggctttc accatgctgt tgctgatgcc gtttgcctgg ggcgaaccgc tgttgcatat
    22441 ctggctgtta ggtattcgta tcgacgccaa cgtcatgcaa ggcatctggc aaatgaccaa
    22501 acagggcgat acgataacgg gggcgatggt ctttttctgc gttattggtg cccccctcat
    22561 tctggtgtcc tccatagcat atttatggtt tggtaaccga ctgggaatga atttacgtcc
    22621 ggtactgctg atgcttgagc gacttaaaga gtgggtaatg ctggatatct acctggtcgg
    22681 cattggcgtt gcttctataa aggtacagga ttatgcccat atccaggcgg gtgtcggctt
    22741 gttctctttt gtggcgttgg tgattttaac gacggtgacg ttgtcacatc ttaatgtcga
    22801 ggagctgtgg gagcgatttt atccgcagcg ccccgctacg cgtagggacg agaaacttcg
    22861 tgtctgtctt gggtgccatt ttaccggcta tcctgatcag cgtggtcgct gcccgcgttg
    22921 ccatatcccg ctacgcctgc gtcgccgtca tagtttgcaa aaatgctggg cggcgctgtt
    22981 agcgtcaatc gttttgttgt tacctgccaa cctgttgcct atttctatca tttatctgaa
    23041 cggaggacgg caggaagata caattctttc cggaattatg tcgctggcaa gtagcaacat
    23101 tgcggttgcg ggaatcgtgt ttatcgccag tattctggta ccgtttacta aagtgatcgt
    23161 catgttcact ttactgttga gcattcattt taaatgccag caaggtttac gcacacgcat
    23221 tctgttactg cggatggtga cctggattgg tcgctggtcg atgctcgacc tgtttgtcat
    23281 atctttaacc atgtcgctga ttaatcgcga tcagatcctc gcttttacta tgggaccggc
    23341 tgcgttttat ttcggcgcag cggtaatttt gactattctt gctgtggaat ggctggacag
    23401 ccgcttactt tgggatgcac atgagtcagg aaacgcccgc ttcgacgact gaagcgcaga
    23461 ttaaaaataa acgccgtatc tcacctttct ggctgctgcc gttcatcgcg ctaatgattg
    23521 ccggttggct gatttgggac agttatcagg accggggtaa taccgtcacc atcgacttta
    23581 tgtcggcgga tggtattgtc ccgggccgta cgcctgttcg ttatcagggc gttgaagtcg
    23641 gaacagtgca ggatatcagc ctcagcgacg atcttcgtaa gattgaagtc aaggtcagca
    23701 tcaagtccga tatgaaagat gcgctgcgcg aagagactca gttctggctg gtgacgccaa
    23761 aagcatcgtt ggcaggtgtc tccgggctgg acgccctcgt cggtggtaac tatatcggca
    23821 tgatgccagg taaaggtaaa gagcaggatc actttgtcgc actcgatacc caaccgaaat
    23881 atcgactgga caatggcgat ctgatgatcc acctgcaagc ccccgatctc ggttcgctga
    23941 acagcggttc attggtctat ttccgcaaga tccctgtggg aaaagtctac gactatgcca
    24001 tcaatcccaa caagcaaggc gtggtgattg atgtcctgat cgagcggcgt tttaccgacc
    24061 tggtgaaaaa aggtagccgt ttctggaacg tttccggcgt tgatgccaac gtcagtatca
    24121 gtggcgcgaa ggtgaaactg gaaagtctgg cggcactggt taacggtgcg attgccttcg
    24181 attcacctga agagtcgaaa cctgccgagg cggaagatac ctttggtctg tatgaagatc
    24241 tggcccacag ccagcgtggc gtaataataa aactggaact gccgagtggg gccggattaa
    24301 ccgccgactc gacgccgtta atgtatcagg ggctggaagt cggacagctg actaaactgg
    24361 atttaaatcc tggtggtaaa gtcactgggg aaatgaccgt tgatcccagc gtcgttacgc
    24421 tgttacggga aaatacccgc atcgaattac gcaacccgaa attatccctt agcgatgcca
    24481 atctcagcgc cctgctgact ggcaaaacct tcgagttggt acccggcgat ggcgagccac
    24541 gcaaagagtt cgttgttgtg ccaggcgaaa aagcactgct gcatgaacct gatgttctga
    24601 cgctgaccct gaccgcgccg gaaagttacg gtattgatgc gggtcagccg ctcattcttc
    24661 acggcgtgca ggtaggccag gttatcgatc gtaaactcac cagcaaaggc gtcaccttta
    24721 ccgtcgccat cgagcctcag catcgagaac tggtaaaagg cgatagcaaa tttgtcgtca
    24781 acagccgtgt cgacgtgaag atggggctgg atggcgttga gtttctcggt gccagcgcct
    24841 cagaatggat taacggcggg atacgtattc tgccgggcga taaaggcgag atgaaagcca
    24901 gctatccact gtatgccaat ctggaaaaag cgctggagaa cagccttagc gatttaccca
    24961 ccacaaccgt gagtttgagt gcagagacgc tgccggatgt gcaggcagga tcggtagtgc
    25021 tctaccgtaa atttgaagtt ggtgaagtta ttaccgtccg tccgcgagct aacgcgtttg
    25081 atatcgatct gcatattaag ccggagtatc gcaaccttct gaccagcaat agcgtgttct
    25141 gggcagaagg cggggcgaaa gttcagctga atggtagtgg cctgaccgta caggcatccc
    25201 cgctctccag agcattaaag ggagccatta gcttcgataa cctcagcggt gccagcgcca
    25261 gtcagcgtaa aggcgacaaa cgaattctgt atgcttccga aacagcggcc cgtgcggttg
    25321 gtgggcagat tacgcttcac gctttcgatg ccggaaaact ggcggtcggg atgccaattc
    25381 gctatctcgg tattgatatc gggcaaatcc agacgctgga tctgattacc gcgcgcaatg
    25441 aagtacaggc aaaggcggtg ctctatccgg aatatgtcca gacctttgct cgcggtggta
    25501 cgcgcttctc agtggtcaca ccgcaaattt cggcagctgg cgttgagcat cttgatacta
    25561 tcctccagcc gtatatcaac gtcgaaccag gccggggcaa tcctcgccgc gactttgaat
    25621 tacaagaggc caccattact gattcgcgtt acctggatgg cttaagcatt attgttgaag
    25681 cgccggaagc cggttcgtta ggcatcggta cgcctgtgct gttccgtggt ctggaagtcg
    25741 gtacggttac aggaatgacg ctggggacat tgtcagatcg cgtgatgatt gcgatgcgca
    25801 tcagtaaacg ctatcaacac ctggtgcgta acaattccgt cttctggttg gcatcgggtt
    25861 acagtctgga ctttggtctg acgggcggcg tagtgaaaac cggcaccttt aaccagttta
    25921 tccgtggcgg catcgccttc gccacgcctc cgggtacgcc actggcaccg aaagcccagg
    25981 aaggcaaaca cttcctgttg caggaaagtg aaccgaaaga gtggcgtgaa tggggaactg
    26041 cgcttcccaa ataatgccca ctgctccggc gtgcctgcgc cggagcgttt atgctaaact
    26101 gcgcgcctgt ttttttgcca gtggtacatg ctcgtggccc aacacaccgt ttatttcccg
    26161 gacgcctttc tgacgcaaat gcgcgaagcg atgccttcga cgctctcttt tgatgatttt
    26221 cttgccgcct gtcagcgccc gttgcgccgc agcattcgcg ttaatacgct gaaaatctcc
    26281 gttgctgatt tcctgcaatt aaccgctcct tatggctgga cgcttacgcc aattccgtgg
    26341 tgtgaagaag gtttctggat tgaacgcgac aatgaagatg cattgccatt gggtagtacc
    26401 gccgagcatt taagcggcct gttttatatt caggaagcca gttcaatgtt gcccgtcgcc
    26461 gccttgtttg ctgacggtaa tgcaccacag cgggtgatgg atgtcgctgc tgcgccaggc
    26521 tccaaaacga cgcaaattgc cgcgcggatg aataacgaag gggcaatcct tgccaatgag
    26581 ttttccgcca gtcgggtaaa agtgttacat gccaatatca gccgctgtgg catcagtaat
    26641 gttgcgctca cacattttga tggccgcgtg tttggtgtgg cagtgccaga aatgttcgat
    26701 gccattttgc tggacgctcc ctgctctggc gaaggcgtgg tgcgtaaaga tcccgatgcg
    26761 ctaaaaaact ggtcaccaga aagcaatcag gaaatcgcag ctacacaacg ggagcttatc
    26821 gacagcgcct ttcatgcatt acgtcctggt ggtacgctgg tttactcgac ctgtacctta
    26881 aacagggaag aaaacgaagc cgtttgcatg tggctgaaag agacttaccc tgacgcagta
    26941 gagtttttac cacttggcga gctcttccct ggtgcaaaca aagcgctgac cgaagaaggc
    27001 tttttgcatg ttttcccaca aatttacgac tgcgaaggct tcttcgttgc tcgtctgcgt
    27061 aaaactcagg ccattcccgc cttacccacc ccaacataca aagtgggcaa ttttccgttc
    27121 agcccggtga aagatcgcga agctggacaa attcggcagg cggctgcagg tgttggctta
    27181 aactgggatg aaaacctgcg cctctggcag cgtgacaaag aactgtggtt gttcccggtg
    27241 ggcattgaag ccctgatcgg taaagtccga ttttctcggt tggggattaa acttgccgag
    27301 acgcacaaca aaggttatcg ctggcagcat gaagcggtta ttgctcttgc ctcacccgat
    27361 aatatgaatg cattcgagct gacaccgcag gaagcggagg agtggtatcg cgggcgcgat
    27421 gtttacccgc aagccgcgcc agtggcggat gatgtattgg ttactttcca gcatcaaccg
    27481 attggtttag ccaaacggat tggttcgcga ttgaaaaaca gctatccgcg tgaactggtg
    27541 cgcgatggga aactttttac cggtaacgcc tgacagcgca caaaaaaagc gcactttttg
    27601 actggcacat tcggcggcct caactaggct gaaaaatggt gcgatcggac tggtcgtacc
    27661 acaatcggca gctaaatgga gagcacgaag atgaaaacca gtgtgcgcat aggcgctttt
    27721 gaaatcgacg acggcgaatt acacggtgaa tcgccgggtg atcgaacgtt aaccattcct
    27781 tgtaaatctg accccgattt atgtatgcaa ctggatgcct gggatgctga aaccagtatc
    27841 cctgccctgc taaatggcga acactctgtc ctttaccgta cccgttacga tcaacaatct
    27901 gatgcctgga ttatgcgtct tgcctgatcc aaaaagaacc cgtcggcatg gcgggttatt
    27961 tgtcctggtt attcccccgt tgtaaaatct ctcctaaact taacggtacg gcaccacact
    28021 tcggggatga aatgttcgcg ctggtacttt ttgtttgcta cctggatggc ggttgtgaag
    28081 atattgttgt ggatgtctac aacacggaac agcagtgtct tcattctatg agcgatcaac
    28141 ggatccgcca tggcggttgt tttccgattg aggattttat agatggtttc tggcgacctg
    28201 cacaggagta cggtgatttt taattattgc aattgcacaa gagtcagttc gcccccaaag
    28261 acagcaccgg tatcaatata atgcaggttg ccaatatcca cgcgatgtcg caacggtgta
    28321 tgaccaaacc agaaatgatc agcacctgta attccctgca ctttttggcg ttcacttaat
    28381 cgcgagcggc tccacaagac ctgatgcaaa tcgacgtcct tttgccattc ataaacatca
    28441 tctggataat cggcatgagc aataacatgc ttgccggtgc gactgtgtac ttcaagaata
    28501 aagggcaaat gctgacattt ccccagcgcc gttttcgctt gtttctgttg attatctgcc
    28561 agcgcaataa accagtcgcc gccattcatc aaccataaag acatctgctg ggatgccagc
    28621 gcatccatcg ccatctgttc atgattgcct cttaccgcac gaacccaatg ttgttccagt
    28681 aactgcagac aacgtaaact ttgcggccca cgatcgataa cgtctcccac tgagataagt
    28741 aaatctcgcc acggatcaaa acgacaatgc cataatttgc ggcgcaactg ctcaagacaa
    28801 ccgtgtatat cgccagaaag ccagatatgt cgccattgat gacccgcaat tctctgataa
    28861 acgggcgcag gctgtttcat caatattttc ctcccgcgct aaagatcaca taatcttaac
    28921 aagaatgtta aaaaacgctg gactcagaca gtagagtgtg tgttatggtt gactataaag
    28981 tcagcgaagg aaatgcttct ggcttttaac agataaaaag agaccgaaca cgattcctgt
    29041 attcggtcca gggaaatggc tcttgggaga gagccgtgcg ctaaaagttg gcattaatgc
    29101 aggcttagtt gccttgccct ttaagaatag atgacgacgc caggttttcc agtttgcgtg
    29161 caaaatggtc aataaaaagc gcggtggtca tcagcttaaa tgttaaaaac cgcccgttct
    29221 ggtgaaagaa ctgaggcggt ttttttattg gaaatcaaaa ggctatttta ggtaattaac
    29281 agagtttttc agctcgttct ataaacggtg ccagactcat tttttcgccg ggattgttag
    29341 gatcatcaat ctgaatcacc gaaatgggtt gggctttagt cttcccactg gcaacttcct
    29401 tttgtgcgat atcgtttaaa ggatactgca cgagggtact tgggttgatg acatacaaag
    29461 cattacccgg tcggcaagtc agcatcacct cttcgcgatt aaacgcccat ttgtctttac
    29521 ccacttcaaa acggctgaca gtaatcacct gcggtgcagc cagcgccgct gcagaactgg
    29581 tgagtaacag aaacgccaga atactttttt tcatcatttt cgtaatccat caaaaaggtt
    29641 cgagagtcgc aagcaggctg atgatcacca gcactaccgc acctatcgcc cattcgagtt
    29701 tagtcatcca cacaaaccag ggtgccgcac gatcttcatc ctgcctcatg cgcggtacaa
    29761 gaacataacg attagccagc gcaatgacca ccataatcat caccaggatg gctttgagca
    29821 ataagagttg gccccagtaa gtcgtaagca tggggggaaa tccggtaatc aacaatgcat
    29881 taagcacgcc gcttgccagt acgccgatca ccgcaaaatg cccgcaccag gaaaaacgca
    29941 tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat gagttgcata caccagagca
    30001 ccggcagtaa tcccccaaac cacgccgccg cacaaatcag gtgaatcgcg tgattagtct
    30061 gatggatttt cgccgttacc ccttcattca gcgtcgcatg ccccactccc gccagcagaa
    30121 taaattgcgc ggtagtgagc ataaacagca aacgtggcat attccgcggt tgcataaggg
    30181 cgacgatcaa cgttacgagg gcgagaacaa tttgccataa ccagacgcca ccaaactgcg
    30241 tttgcaaaac agcctgccaa atgtttggcg aaaatacatc tgtccatccc gttcccatca
    30301 acccgccctg aacggcgagc attgcagtgg cactgatcaa actccagacg gcagcgtgct
    30361 gttgtaaacg cagaaaacgc ttcgccagca agcgacgaat cgttaagggg gccagccagg
    30421 caccgtacat tgcgaagcca aaaaccagca tcagcgaggt gaaatggata aatcgcagcg
    30481 cgatccaggt aaacgccagc atgatttatt tcacgctaaa ggtgtagtgt cctttcgttt
    30541 tatgcccgtc caccgaaaca acatgccagt caacggtgta ggttccgggt ttcagcgaat
    30601 cggccagtgg aacaattagt tgctttgggt cctgctcatt tcgcttcgcc ggtaatgttt
    30661 taatattttc gttttttggc cccgtgattt ttgcaccact gaatcctgtt tcaacgcctt
    30721 ccgagaagtt taaagtgatt gcctgcggag atgctttcac ttgcgcgttt gccgcaggat
    30781 actgatgtat taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa gtggtcagga
    30841 ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat attatctatt cctttttgta
    30901 ataacttttt tacagagcat aaccttgtct aatgtctgag tcgaggatca tcaattccgg
    30961 cttgccatcc aggctcactc ttagtaactt ttgcccgcga atgatgagga gattaagaat
    31021 gctgaagaat ctggctaaac tggatcaaac agaaatggat aaagtgaatg tcgatttggc
    31081 ggcggccggg gtggcattta aagaacgcta caatatgccg gtgatcgctg aagcggttga
    31141 acgtgaacag cctgaacatt tgcgcagctg gtttcgcgag cggcttattg cccaccgttt
    31201 ggcttcggtc aatctgtcac gtttacctta cgagcccaaa cttaaataaa acttatacag
    31261 agttacactt tcttacataa cgcctgctaa attatgagta ttttctaaac cgcccccata
    31321 atttgcagtc attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc agcagcgcaa
    31381 tatgagcctc gtaaaacctc actcaccgaa caggtagctc atcatctgga gtttgtgagg
    31441 gccgccgcca ggcaacaatg tcagctgtta gtttttccat cgctttcttt actggggtgt
    31501 gattattcgc gacgtgccct tcctgcccca cccgatctct cactgttaga cccgctttgt
    31561 tatgccgcaa cgacctggcg aatgaccatc attgccggcc ttcccgttga atataacgat
    31621 cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa aaacgcctgg gatctaccat
    31681 cagagtcatg gtgcatgtct gggccgtcgt tccagaacca tcacggtggt cgatgaacaa
    31741 ccgcaaggta tggatatgga cccgacctgt tcactgttta cgacagggca atgtctggga
    31801 gaacccgacc tgctggcgtc ggcccgtcgc ttacaatttt tttcacatca gtactcgatt
    31861 gccgtgctga tggcaaatgc ccgtggtaac agtgcactat gggatgaata tggtcgtctc
    31921 atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc acagggttgg
    31981 caaggcgata tcattccatt acgctaggct ttttcggcct ggagcatgcc atgttgcgca
    32041 ttatcgatac agaaacctgc ggtttgcagg gagggatcgt tgagattgcc tctgttgatg
    32101 tcattgacgg aaaaatcgtc aaccccatga gccacctggt gcgccccgat cgtcctatta
    32161 gtccacaagc gatggcgatt catcgcatca ccgaagccat ggtcgccgat aaaccgtgga
    32221 ttgaagatgt gatcccacac tattacggta gtgaatggta tgtcgcgcat aacgccagct
    32281 ttgaccgccg cgtactgcct gagatgcccg gtgagtggat ttgcactatg aaactggccc
    32341 gtcgtttgtg gccagggatc aagtacagca atatggcgtt atataaaaca cggaagctca
    32401 atgtacagac gccgccgggc ctgcatcatc accgcgcgtt gtatgactgt tatatcaccg
    32461 ccgcgttgct tatcgatatt atgaacacct ccggctggac ggcagaacag atggccgata
    32521 tcaccggacg tccgtcgttg atgacgacct tcacctttgg caaataccgt ggcaaagcgg
    32581 tttccgacgt tgccgaacgc gatccgggct atctgcgctg gttatttaat aacctggaca
    32641 gcatgagccc ggagctgcgt ttaacactga aacattatct ggaaaatact taatccgcag
    32701 gcgtagcggg taatgttccc tgcgccagcg cgaccagaaa agcatattcc atcgctacgc
    32761 cttcgtacga tttaaagcga ccagatttac cgccatggcc tgagtccatg tcggtacaga
    32821 gcaataaaag atggttatcg gttttcagct cgcgcaattt agcgacccat tttgccggtt
    32881 cccaatattg cacctgagaa tcgtgcaaac cggtcgttac cagtaaatgc ggataagcct
    32941 gtgcggtgac gttgtcatac gggctgtagc ttttcatgta ctcgtaatat tgcggatcct
    33001 gcgggttccc ccactcttca aactcaccag tagtaagagg aattgattca tcaagcatcg
    33061 ttgttacaac atcaacaaac ggtacctggg cgataacgcc gtggaataat tccgggcgtt
    33121 gattaattgc aacgcccatc aacatgcccc ccgcactccc gcccatcgca taacaaagcg
    33181 aaggagagcc atagcccagt tttaacaatg catcgcaggc atcaagataa tcattaaacg
    33241 tatttttctt cttcagaaat tttccgtctt cgtaccattg ttgccccagc tcaccaccgc
    33301 cgcgaacatg gacaatggcg tagacaaagc cacgatctaa caaactcaag cggctaaaac
    33361 tgaaatcggc atcaatactt gcgccgtaag aaccatagcc atacaccagc aatgggttgt
    33421 gtcctttgcg aaaatgtttg cgatgataga ccagcgaaac cggaacttcg acgccatcgc
    33481 gggcgactat ccacaggtgt tcactgcggt aattcgccgc atcaaaaccg ggcacttctg
    33541 tttgttttaa tacacgacgc tcaccggtat ccatatccag ttcaaacaaa gtgtctggtg
    33601 tggtcatgga agaataacca taacgcaatc gcgctgtttc aggttctgga ttgtaggcaa
    33661 tccaggtcac ataggccgga tcatcaaagg caataccaat gacttcccgg gtcttgcggt
    33721 taatttggcg caaactggtt aacccgcgct gacgctcttc aaccaccagc cagtcggtaa
    33781 acagcgtaaa cccttccagc atgatgtttt cgcgtggcgg aattaactct tcccactgtt
    33841 gctcatcacg catacgggta cggtataagc caaagttttt gccgtggcgg ttggaacgta
    33901 gataaaaacg atgctggtag tgatcaaggc tgtattcgtg atctttgcgg cgcggcagaa
    33961 aaacaaacgg ctcggcatcg gccatttccg cgtccagcag gcgaacttca ctggtggtgg
    34021 cgctggccaa atgaatgact acatagtgct tcgaggtcgt tttatgcagg ctgacgtaat
    34081 aggtatcgtc tttttcttcg tagatcagtt tatcttgcga tgctggcgta ccgatggcgt
    34141 gacgccagac ctgataaggc agcagcgtca ccggatgctt gcgaacatag tagaaagtcc
    34201 aggagtcatt tgcccagaca aagctgggtt caacgttatc cagcagttcc gggtaccagt
    34261 taccagtttc cagattacga aaacgaatgc cgtactggcg tcgggaaaga aaatcttctg
    34321 ccagcgccat aatggtgtta tcgggcgtaa tcgccattcc gcccatcgaa taaaactcac
    34381 tatgagctgc gcgcttattg gcatcgagca atgtttccca ctcatcccac tcttcactga
    34441 atgccgattg acgctggtag atagcatatt cacagcctgg ttcataaata tgccgatagc
    34501 ggtagccatt tttgatgtag ggcgcagaaa cttctcgttg cggaatgcgg tcgatgattt
    34561 cctttaagat gcgatcctgc aaggcttgtt gtgaggccat cacccgatga ccgtaactat
    34621 tttcttgttg caggtagtcc agaacttccg gctgagaacg cgtatcgtcc cgcagccagt
    34681 agtaattatc gattcgcgta tcgccatgaa gcgtcatggc gtggggaatg cgggcggctt
    34741 ttggtagcat gttattgttc tttctggttg aaacatctta taagggtggc aaaactcacc
    34801 ggggatgcaa gcgaaacagg ggagtcattg cttagatgat gacaggtaat ggcgcggata
    34861 tcgaatgtta tgcagacaga gaaaatcagc ctgttcaaat ggctgtgcga ttctggatag
    34921 cccgaaatag tcaacttcag gctatccaga gagcggaatt attccgccaa agtgcgtttt
    34981 tgctgttcga gatcgcgttc aatgccgtca cgaacatcct gggggatttt cagcgcgtca
    35041 cccagtgcat tcaggtaact gcgttccata aaatggtcaa tatcaatagc cgcgcaactc
    35101 aggaaataga tttccagcgc ctcttcttca ttgcggaccc cggtagccag gcgttgtgga
    35161 tccagcggtt gttcgattgc ctgctcaatg agtacacgcc cctgctcttc cacgccggct
    35221 tcacgcaatt gctggtcgat tgccgcacgt tctttggcat caatatgacc atcactttta
    35281 gcggcaaaga ccagcgcaag gatcaaacgt tctgtacgct catccagcgg cgtactttgc
    35341 gcgccaaact gcggttcgtc ctgatgcgcc gcgcgaattt tatctttgta tttattccac
    35401 agcaccgtac ccgctaccgc tccgccgcca accagtaacg cgttggtgcc atatttcgta
    35461 agaagtttac gtgctgattt atttgcgacc agcagcccag ccagtccgcc taatgcgcct
    35521 ggcactaaca gtttgcccaa tccctgatcc gcagacgagg aggtagaaga actgctttgc
    35581 ccaagcaggg attgcagttg atttaaccag ttagccatat ttgctcctca ataaccattt
    35641 ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg tatatcgaga aaaaagatgc
    35701 gcaaataagc ggggcggtag gccgttattc aaagaaattc gcgccaggta aggtatttac
    35761 tgtatatcag gtgataaggg tattttaggt gaaagtagtt tgtcgttttg agtggcattg
    35821 tttgatgctg attgcccgat gcgacgctgt tgcgtcttct caggcctacg attgtggtgc
    35881 gttctggggc ggacaaggca ctcgcgccgc atccgccaac ggtcaatcga attatcagaa
    35941 gccattaacg ccgctgatat tccgccattc ccgctttgca gtccacgctg acctggtaat
    36001 gaatatcggc acttttacca cgcacggtta gcggtactga ccatttatca tctttaccct
    36061 gaatgtcctg caaactgacc caagccacgg gatcggcctg accgacaatt ttttgatcat
    36121 ctgcccaacg cgccacgcga ttttgttgat aatcacgttt tacgctcgcg gcaattccgg
    36181 cagcatccag accttcacac tttgggaaag tgaccgactt gctggtttca ttattggcag
    36241 cgaaaactga cgcgcaggca gaaaccaaca acagccctaa aaacgcccct ctttttttca
    36301 tgtttttctc catagcacaa tgattcagga gaaagcatgg tacaaattgt caggagcgca
    36361 agttgcttca ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg acgacctgtg
    36421 gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt tctgccagcc
    36481 atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaattt gtcagccagg
    36541 tgtcaagcag atccgccgta tcaagcattt tgtcataagc atcggcttcc tttttgctgg
    36601 taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca accgccattt
    36661 gcagcctctc ataataactg tgattttata cagtatattt cttttcggtt gagaaatcaa
    36721 catcagcaat aaagacacac gcaaacgttt tcgtttatac tgcgcgcgga attaatcagg
    36781 ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact cgcgtgatgt
    36841 tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt ctcggcgtag
    36901 aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg catcgctccc
    36961 atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg gaaaaaccac
    37021 attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa cttgaagagg
    37081 aaggactaaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat cgcgaaggta
    37141 tccgtcgcct ggcggcagaa gagctgcagc tgcccacttc cacttatcgt tttgccgata
    37201 gcgaaagcct tttccgcgag gcggttgccg ccattggcta tccctgcatt gtaaaaccgg
    37261 tgatgagctc ttccggcaag gggcagacgt ttattcgctc tgcagagcaa cttgctcagg
    37321 catgggaata cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt gttgaaggcg
    37381 tcgttaagtt tgacttcgaa attaccctgc taaccgtcag cgcggtggat ggcgtccatt
    37441 tctgtgcacc agtaggtcat cgccaggaag atggcgacta ccgtgaatcc tggcaaccac
    37501 agcaaatgag cccgcttgcc cttgaacgtg cgcaggagat tgcccgtaaa gtggtgctgg
    37561 cactgggcgg ttatgggttg tttggtgtcg agctatttgt ctgtggtgat gaggtgattt
    37621 tcagtgaggt ctcccctcgt ccacatgata ccgggatggt gacgttaatt tctcaggatc
    37681 tctcagagtt tgccctgcat gtacgtgcct tcctcggact tccggttggc gggatccgtc
    37741 agtatggtcc tgcagcttct gccgttattc tgccacaact gaccagtcag aatgtcacgt
    37801 ttgataatgt gcagaatgcc gtaggcgcag atttgcagat tcgtttattt ggtaagccgg
    37861 aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt gttgttgacg
    37921 ccattgaacg cgcgaagcac gccgccggac aggtaaaagt acagggttaa acccggcaaa
    37981 aaaacgctac aaaaatgccc gatcctcgat cgggcatttt gacttttaca gcttagcgcc
    38041 ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc
    38101 tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca ggtagtcacg
    38161 gtagttagcc ggagaaatac cacccgtcgg gcagaaacgg acctgggaga acggacccgc
    38221 gatcgcctgc agggctttca cgccgccgtt agcttcagcc gggaagaatt tgaactcttt
    38281 caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg gaatcagagg
    38341 aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc taattgcgaa
    38401 ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg taccggcacc
    38461 cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg cacactcggt
    38521 acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg ccatcggcac
    38581 cgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg tcaggattga
    38641 ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta caaatttgtc
    38701 gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc
    38761 gctgaataat tcacgtcctg tccccacgcg tgacgcgctc aggtcaggaa tgtgcggttc
    38821 gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct gtccattcac
    38881 acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg cttctggtgt
    38941 tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat cggtaactaa
    39001 cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt tatgtaattc
    39061 tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat cgcggtccag
    39121 caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc
    39181 ttcaatcacc tggttctcaa ccggcacggc agaggttttc ataaccgcac ggcccaggtt
    39241 accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg aagcgatcac
    39301 attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat tattcagcca
    39361 tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat cttcatgcag
    39421 caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg cctggaagtg
    39481 gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa catcagaaag
    39541 gtcagagaag tcatcccagt taatctgaat accggccgcg cgcgccatcg ccaccaggtg
    39601 catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt tcaccaccac
    39661 tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg tcatgcgtgt
    39721 aacctgacgc gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa
    39781 agaagagcct ggcaactgca tccccataaa ctccaccacc atctggttgg tgttggcagt
    39841 accgtagaaa gtacatgttc ccggcgcatg gtaagacgcg gcttctgact ccagtaaggc
    39901 catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt ctttatttgg
    39961 caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgac caaacgacag
    40021 ggctgccatc gtcagacccg ggacaatctt gtcgcacacc ccgaggaaca gagcaccatc
    40081 aaacatgtta tgggacagcc cgaccgccgc agacatcgct atcacttcgc ggcttagcag
    40141 cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg gaacaccgcc
    40201 cgcaacctga ccaaccgcat tcgcttcatg cagggcttta cgaatgattt ctggatagtg
    40261 ttcataaggc tggtgagcgg agagcatgtc gttataggag gtgatgatgg cgatattgtt
    40321 acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga aaccgtgtgc
    40381 caggttaccg catgccaact gcgaacgatg aacggtcgac gttttcgctt gttctatccg
    40441 ggcgagataa gcagagcgag tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa
    40501 caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg attcacaacg
    40561 cgtttcattc agaggattta tgactgaaac gcctgtaacc ggagctcata gggcaaaaac
    40621 gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt
    40681 aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca taaaggataa
    40741 gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca tcgccaccga
    40801 ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt ccatcgccca
    40861 cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcgtcgc gacgtacaaa
    40921 cagtgcctga ataccacgca tggtttccag cagcaaacgt tcataggcat ccgccagatg
    40981 cgtctgatta aaggtttctg aatagctcag atccagcttg gtgatttgca ggttatgttt
    41041 gtggtcaagg ccaggaactt tattcagtac ctggatatcc acgccttcat caggttgcag
    41101 acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca gattcagttc
    41161 aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt taccagtacg
    41221 caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa
    41281 ggtttctgta ttgctgctct tgttcgcgcc ctcttcttcc agatatcccg gcactttttt
    41341 gccctgggcg aagcccgcag tatattgccc gcgtacggtt ttttcgcgta cgttggagcg
    41401 gtcgatgcgg cgcagagact tcagtacttt cactttttca tcgcggatgc tgtctgcgct
    41461 caggtcagac ggcggagaca tcgcaatcat gcaaagaatt tgcagcaggt ggttctggat
    41521 catgtcgcgc atctgaccgg ctttatcaaa atagccccag cgcccttcga tccccacttc
    41581 ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat tcacaaacag
    41641 ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc
    41701 gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcattga tttcctgcga
    41761 ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt tagcattcag
    41821 ttttgcctcg ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta
    41881 gttaatggtg atacgatttt tttgatccag catcgcgccg agacggctga atgcagcagt
    41941 gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc ataaaccttc
    42001 atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg tatatgccgc
    42061 tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga gctgaccggc
    42121 tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc
    42181 gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat tctccttaag
    42241 ttaactaacc cggtacttaa gccagggtat acttgtaatt ttcttacggt gcactgtact
    42301 gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta cgggaaaagc
    42361 gcgcaaagtg cggcaaaact gataaaaaaa tcatcgtttt ccattgggta aaaatctgac
    42421 actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca ttaacacggc
    42481 acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagtgcgg aaatcgtcat
    42541 tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc agctggaaca
    42601 tttgagcaaa tcagagcgca aagttgccga ggtcattctg gcttcgcccg ataacgcgat
    42661 ccattcgagt attgctgcta tggcactgga agccaatgtt agcgaaccga cggtgaatcg
    42721 tttctgtcgc agcatggaca cgcgcggttt tcctgatttt aaacttcatc tggcacagag
    42781 tctggcgaat ggcactccct atgttaatcg caatgtcaat gaagatgaca gcgttgaatc
    42841 atacacaggg aaaatatttg agtccgcaat ggcaacgctt gatcatgtcc gtcattcact
    42901 ggataaatct gccatcaacc gcgccgtcga cttgctcact caggcaaaaa aaatcgcctt
    42961 tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt tctttcgttt
    43021 taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt gtatgaattg
    43081 tagcgacgga gacgtggtgg tgctgatttc tcacactgga agaacaaaaa atctggtcga
    43141 gctggcgcag ctggctcgcg aaaacgacgc catggtgatt gccctcacct ctgcgggtac
    43201 cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag atactgacat
    43261 ttatatgccc atggtttctc gacttgcaca gctgaccgtg atagatgtgc tggcgacagg
    43321 atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca aggaagcgct
    43381 gaaggaatcg cgttttgata agcagttact taatttaagt gacgatcgct aaaaacgact
    43441 gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca tcgcggcgtt
    43501 atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg gagtattaca
    43561 tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca acagatcgcg
    43621 ataataacct tgaaaaagtt atcgcggcag gtgccaacgt tgtacgtatg aacttttctc
    43681 acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgagatt gccgcaaaac
    43741 tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaatccgt gtatccacct
    43801 ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac gccaacctgg
    43861 gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg cctgctgacg
    43921 tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt ccagttaaaa gtactggaag
    43981 ttcagggcat gaaagtgttc accgaagtca ccgtcggtgg tcccctctcc aacaataaag
    44041 gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa gacaaagcag
    44101 acattaagac tgcggcgttg attggcgtag attacctggc tgtctccttc ccacgctgtg
    44161 gcgaagatct gaactatgcc cgtcgcctgg cacgcgatgc aggatgtgat gcgaaaattg
    44221 ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat gacatcatcc
    44281 tcgcctctga cgtggtaatg gttgcacgtg gcgacctcgg tgtggaaatt ggcgacccgg
    44341 aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagctaaac cgagcggtaa
    44401 tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg cgtgcagaag
    44461 tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctg tctgcagaaa
    44521 ctgccgctgg gcagtatccg tcagaaaccg ttgcagccat ggcgcgcgtt tgcctgggtg
    44581 cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag ttcgacaatg
    44641 tggaagaagc tattgccatg tcagcaatgt acgcagctaa ccacctgaaa ggcgttacgg
    44701 cgatcatcac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt atcagctctg
    44761 gtctgccaat tttcgccatg tcgcgccatg aacgtacgct gaacctgact gctctctatc
    44821 gtggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcagct gccagcgaag
    44881 cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg attgtcaccc
    44941 agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta acggtagagt
    45001 aagtacgttg ccggatgcgg ctaaaacgcc acatccggcc tacagttcaa tgatagttca
    45061 acagatttcg aatattctga agcaaacttg aacttatcat caggcgaagg cctctcctcg
    45121 cgagaggctt tttttatttg atgggataaa gatctttgcg cttatacggc tggatttcgc
    45181 ccggtttgcg agttttcagc aattttagta tccaggtgta ttgttctggt cgcggaccaa
    45241 caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc gcctctaaca
    45301 gatcatccat cggtgggcgc acctgaatcg tcagacgatg cgtcttgcca tcataaatcg
    45361 gaaacagcgg taccacgcgc gcacggcaca ctttcatcaa acgaccaatc gcgggcaacg
    45421 tcgctttata ggtggcaaag aaatccacaa attcgctgtg ctctgggcca tgatcctgat
    45481 cgggtaaata atatccccag tacccctgac gtaccgactg gatgaatggt ttaataccgt
    45541 catttctcgc atgcagacga ccgccaaagc gacgacgcac cgtgttccag acataatcaa
    45601 aaaccgggtt gccctgatta tggaacatcg ctgccatttt ctgcccttgc gaggccatca
    45661 gcatggcagg aatatcgacg gcccaaccgt gcggcaccag aaagataact ttctcgttat
    45721 tacgccgcat ctcttcgatg atctccagcc cttgccagtc aacgcgcggc tgaattttct
    45781 ccggcccgcg tattgccaac tcagccatca ttgccatcgc ttgcggcgcg gtggcaaaca
    45841 tctcatcaac aatcgcttcg cgttcagctt cactacgttc tggaaagcag agcgacagat
    45901 tgattaacgc acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt cccagccgtg
    45961 ccaaaatggg atcacggaac tttggcggcg ttaaagcgat acccgccatc gctgctacgc
    46021 ccagccatgc tccccagtag cgcgggtggc gaaaggattt atcaaactca ggaatgtatt
    46081 cgctattatt ttttttcgtt tccatgcttt tccagtttcg gataaggcaa aaatcaatct
    46141 ggtgatagtg tagcggcgca acttgccccg caccaaataa aaaaagccgg tactgactgc
    46201 gtaccggctg cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa tctctttggc
    46261 ctgcgccagg aattcgcgac gatcggagcc ggtcagccct tcggtacgcg gcagttttgc
    46321 cgtcagcggg tttacggcct gctggtttat ccatacttca tagtgcagat gcggcccggt
    46381 tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca cgtttcacct tctgtcccgg
    46441 tttcaccaga atcttgcgca agtgcatata acgcgtggtg tagctgcgac catgacgaat
    46501 agccacataa taacctgctg cgccactgcg tttggcaacc accacttcac cgtcacccac
    46561 tgaaagcact ggcgtaccct gcggcatggc gaaatcaaca cctctgtgtg gtgcaacgcg
    46621 accggtcacc ggattagtac gacgcgggtt aaagttagac gagatacgga actgtttcgc
    46681 cgtcgggaat cgcaagaatc ctttcgccag accagtaccg ttacgatcgt agaatttgcc
    46741 atcttcagcg cggatagcgt aataatcttt accttctgaa cgcaaacgta cgcccagcag
    46801 ctggctttgc tcacgtttac catcaagcat ttctcgagac attaacaccg caaattcatc
    46861 gccttttttc agtttgcgga aatccatttg ccactgcatg gctttaatca ctgcgctcac
    46921 ttcggcgctg gttaaaccgg cgtttctggc gctggcaaca aagctccccc cgacggtacc
    46981 tttcagcaga ttgttaaccc actctccttg ctgcatttcg ctggtcattt taaaaccgtt
    47041 agcggcagta cggtcatagg ttcgggtttc acgacgagac acttcccagg tgaggcgctg
    47101 cagttcgccg tccgcggtta atgtccagga gagttgttga ccgattttca ggttacgcaa
    47161 ttctttgtcg gccgcagcca gttgggtgat atcacccata tcaataccat actgattgag
    47221 aatgctgctt agtgtatcgc cagtggaaac aacatattca tgcacgcccg cttcaccggc
    47281 gattttgtca tccagttcgt cctgaggaat ggcttcatct tcttgtgcag cttgatcaat
    47341 cggctcactg gcttcaggta agagcgaacg aatttcgttc tgttccagct caatggtttt
    47401 gacaattggc gtggcatcgc ggtgataaac atagggccgc cagacagcga cggccagagt
    47461 aagaacggtg agcgacccca acataacgcg gtgtggtcgc ggtaaattat taaacgccag
    47521 ggcgacagag cgggctatct gttgcacgta atcacttcct cattaatctc ctttcaggca
    47581 gctcgcatac tggttggcta attgactcag gaattctgaa tagcttgttt tacccagttt
    47641 gatattcgtc cccaggggat ccaacgttcc catacgaacg gatgtccctc gtgcgacgct
    47701 ctcaacgacc gctggcctga actgtggctc agcaaaaacg caggttgctt tttgctcaac
    47761 caactgtgtt cttatttcat gtaaacgctg cgcgccaggt tgaatctcag ggttaacggt
    47821 aaaatgacca agcggtgtca gtccgaactg tttttcgaaa tagccgtaag catcgtgaaa
    47881 aacgaaataa cctttcccct tgagcggcgc gagctcgtta ccaacctgcg tttcggttga
    47941 ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac tttgcggcat
    48001 aagttccact aattttccat ggattgcaac cgctgtagcc cgcgctatct ctggggaaag
    48061 ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg tcactttttt ccgcgtggtc
    48121 gtgatcatca tcatcgccgt gaatactttt catcagtagc ggtttcacat cttcaagctg
    48181 cgcaatcgtt acctgcttcg ctcctggtaa tttgcttacc ggtttttgca taaacgcttc
    48241 catctccggg ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta catccgatgg
    48301 gcgcagtgaa taatcatgtt ctgaagcgcc gtcaggaagt aaaacctctg tttctgttac
    48361 cccatcagca atggcagaag cgatgaaccc aacgggttta agcgaagcga caacggcggc
    48421 atctgcggcc tgtgttgcac ctccccagag agcggcggat aatgctgcga aaagaagcgt
    48481 ttttttatgt aacataatgc gaccaatcat cgtaatgaat atgagaagtg tgatattata
    48541 acatttcatg actactgcaa gactaaaatt aacatgacaa gtctggtttc cctggaaaat
    48601 gtctcggttt cttttggcca acgccgcgtc ctctctgatg tgtcgctgga acttaaacct
    48661 ggaaaaattt tgactttact tgggccaaat ggcgcaggta agtcgacact ggtacgggta
    48721 gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa actgcgcatc
    48781 ggctatgtac cgcagaagct gtatctcgac accacgttgc cactgaccgt aaaccgtttt
    48841 ttacgcttac gccctggtac acataaagaa gatattttgc ctgcactgaa acgtgtccat
    48901 gccgggcacc tgattaacgc accgatgcaa aagctctctg gtggcgaaac gcagcgtgta
    48961 ctgttagcgc gagcattgtt aaatcgcccg caattattag tgctggatga acccactcaa
    49021 ggcgtggatg taaatggcca ggtggcgtta tatgacctta ttgaccagtt gcgtcgtgaa
    49081 ctggattgtg gcgttttaat ggtttctcac gatctgcatc tggtgatggc aaaaaccgat
    49141 gaagtgctgt gcctgaatca ccacatttgt tgttccggca caccggaagt tgtttccctg
    49201 catccggagt ttatttcaat gtttggtcct cgtggtgctg aacaactggg tatctatcgc
    49261 catcatcata accatcgtca cgatttacag ggacgaattg ttttgcgtcg gggaaatgat
    49321 cgctcatgat tgaattatta tttcccggtt ggttagccgg gatcatgctc gcctgtgccg
    49381 cgggtccgct gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt gatacgctgg
    49441 ctcatgcctc attacttggc gtcgcgtttg gtttgttgct ggacgtgaat ccattctatg
    49501 cggtgattgc cgttacgctg ctgctggcgg gcggtctggt atggctggag aagcgtccac
    49561 agctggcgat cgacacgtta ttagggatta tggcgcacag tgccctgtcg ctgggcctgg
    49621 tggtcgttag tctgatgtct aatattcgtg ttgatttgat ggcttacttg ttcggtgatt
    49681 tgctggcagt gacgccggaa gatctcatct ccatcgccat tggcgtggtc atcgtggtgg
    49741 ctattttgtt ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg gatctggcgt
    49801 ttgttgatgg tgtgaaatta cagcgcgtga aattgttgtt gatgctggtg acggcattga
    49861 cgattggtgt agcgatgaaa tttgtcggtg cgttgattat tacttcgttg ctgattattc
    49921 ctgcagctac tgcgcgtcgc tttgcccgta cgccggaaca gatggctggt gtcgctgttt
    49981 tggtggggat ggtggcagtg actggcggtt taaccttttc cgcattttac gatacgccgg
    50041 ctggtccgtc ggtggtgcta tgtgcggcac tgttatttat tctcagtatg atgaaaaagc
    50101 aggccagcta atctatcgct gaacacattt gtcggatgcg gcgcgagcgc cttatccgac
    50161 ctacggttcg ctatctctgg ctggccggat aagacgcgaa tagcgtcgca tcaggcacac
    50221 tgccaatttc ggatgcggtg cgagcgacca atccgactta cggcatttct ggcggcgtta
    50281 tgccaaagtg attccacgcc cgcgtcgtcg ccatacgccc acgcggtgta cgctgcaaaa
    50341 agccttgctg aatcaaataa ggttccagca catcctcaat ggtttcacgt tcttcgccaa
    50401 tggctgccgc aaggttatcc agacctaccg gcccaccaaa gaacttatcg attaccgcca
    50461 gcaacaattt gcggtccata taatcgaaac cttcagcatc gacattcaac atatccaacg
    50521 cctgagcagc gatatctgcc gagatggtgc catcgtgctt cacttcggcg aaatcacgca
    50581 ctcgacgcag cagacggttg gcaatgcgcg gcgtaccgcg agcgcgacga gcaacttcca
    50641 gcgcgccgtc atcactcatc tcaagcccca taaagcgtgc gctgcgactg acgatatatt
    50701 gcagatccgg cacctgataa aactccagac gttgcacaat accaaaacgg tcgcgcaacg
    50761 gtgatgtcag cgaacctgcg cgcgtggttg caccaatcag ggtaaacggc ggcaaatcaa
    50821 ttttaatgga gcgtgccgcc ggaccttcac caatcatgat atccagttgg tagtcttcca
    50881 ttgccgggta cagcacttct tcaacaactg gcgataaacg gtggatctca tcaataaaca
    50941 gcacgtcatg cggttcaagg ttagtgagca tcgcagccaa atcgcccgcc ttttccagca
    51001 ccggaccaga agtcgtgcgt aaattaacgc ccatttcatt ggcgacaatg ttggcaagcg
    51061 tagttttacc caaccccgga ggaccaaaaa tcaacaaatg atcgagggca tcgccgcgca
    51121 gtttcgctgc tttgatgaaa atctccatct gtgaacgaac ctgcggctga ccaacatact
    51181 cttccagtaa tttagggcga atggcgcgat ctgctacatc ttccggcaaa gtggtaccgg
    51241 cagaaatcag acggtctgct tcaatcatcc tttacctcat aacgcggcgc gtagggcttc
    51301 gcgaattaat gtttcactgc tggcgtcagg gcgagcgatt ttgctcacca tgcggctggc
    51361 ttcttgtggt ttatagccca gcgccaccag cgcagcaacc gcttcctgtt cagcatcgtt
    51421 ggttgccggg ctggcaggcg acgtgagcac cagatcggcg gctggcgtaa agagatcgcc
    51481 atgcagacct ttaaatcggt ctttcatttc aacaatcaag cgttcggcgg tttttttgcc
    51541 aatacccggc agtttcacca gcgcccccac ttcttcacgc tcaacggcat taacgaactg
    51601 ctgcgctgac attccggaga ggatcgccag cgccaacttc gggccgacgc cgttggtttt
    51661 gatcaactct ttgaacaacg tgcgctcttg tttattgtta aaaccgtaca gcaactgcgc
    51721 gtcttcacgc accacaaagt gggtgaaaac gatcgcttcc tgtcccgctt cagggagttc
    51781 ataaaaacag gtcatcggca tatgcacttc atagcctacg ccgcccactt caattaacac
    51841 cagcgggggt tgtttttcaa tgatgatgcc tctgagtctg cctatcacat gacgctcctg
    51901 cgtaatgaat caaagataat gctgtatgat aaaaaaatgc tggatagata tccagcgaag
    51961 gatgaagaaa acttgcgagg tgtctcgatg atctgaaaaa tggcacagta taatttattc
    52021 tacaggttat tttggaagca aatattttaa tattacatat tcagtgaaaa aatgcgtaat
    52081 aaaaatatac attgcgcctc ctgaaaaaat aaatttttta tgctattacg tatattcgta
    52141 tctatttcaa tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggaga
    52201 tatcgccttt acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaattg
    52261 ttggagtaat cacgtcggga tcattatcgg tcataacggt gaagacttcc tggttgcaga
    52321 aagccgggtt cccctctcaa ccatcactac gctatcccgt tttattaaac gctctgctaa
    52381 tcaacgctat gctataaagc gattagacgc tgggctgaca gaacaacaaa aacaacgaat
    52441 tgttgaacag gttccctccc ggctacgcaa actttaccac accggtttta aatacgaatc
    52501 ttcgcgccag ttctgttcaa aatttgtctt tgatatttat aaagaggcgc tatgtattcc
    52561 ggtgggtgaa atagagacgt ttggagaatt gttaaatagc aatccgaatg caaaactcac
    52621 tttctggaaa ttctggttcc tgggttctat tccgtgggag cgtaaaaccg ttacgccagc
    52681 cagtttgtgg catcatccgg gtttggtgtt gattcatgcg gagggagtgg aaacgcctca
    52741 gccggaactg accgaggcgg tataacttaa cgcagtcgcc ctctcgccag gttcagccgc
    52801 gattcgctca tctgcatcgc attctgacta acgtggcagt gggtgatggc aatcgccaac
    52861 gcatcagcgg catccgcctg tggattagcg ggcagtttca gcaaggtgcg gaccatatgc
    52921 tgcacctggc ttttttcggc gctaccaata cccaccaccg tttgcttcac ctgacgtgca
    52981 gcatattcaa ataccggcaa ctcctgattc accgccgcca caatcgccac gccgcgcgcc
    53041 tggcccagtt tcagggctga gtcagcgttc tttgccataa agacttgttc aatggcgaaa
    53101 taatcaggct ggaactgggt gatgatttcc gtcacgcccg catagatgag tttcagacga
    53161 gacggtaaat catccacttt ggtgcgtatg catccgctac ccaggtagga cagttgccta
    53221 cctacctggc ggatgacgcc gtagccggtc acgcgcgaac ccggatcaat gccgagaata
    53281 atagccatca cgcgtctccg ttttgctgtt tagcaggcct catcagagag tcgctgcgac
    53341 ctcatcagag atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat
    53401 atcgatcaga cgcatcagtt tcggtgcggt ttctgcatcc atatcagctt tggtagacgg
    53461 gatcatggaa acttccgcgc tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac
    53521 tttacccatt tcttcccatg cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac
    53581 gtcttcagca cctgcttcca gtgctgcttc catgatggtg tcttcatcgc ctttctcgaa
    53641 ggagatcacg ccttttttgc tgaacagata agctacggaa ccatcagtac cgaggttacc
    53701 gccacatttg ctgaatgcat gacgcacttc agcaacggta cggttgcggt tgtcagacag
    53761 acattcaatc atgattgccg tgccgccagg accgtaacct tcgtagatga tggtttccat
    53821 gtttgcatca tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg tgtcgcgggt
    53881 catgttgtta gacagtgctt tatcaattgc tgcacgcaga cgcgggttag cgtccggatc
    53941 gccaccgccc agcttagccg cggttaccag ctcacgaatg attttagtga agattttacc
    54001 gcgcttagca tcctgcgcag ctttacgatg tctggtgttg gcccatttac tatgacctgc
    54061 cataaaaata tctccagata gccctgcctg ttcaggcagc gttaattaca aactgttcaa
    54121 tcgcctgccg gttgctccag gacttagtga gcgccgccgc agcaggcgca tcaagccact
    54181 tgtaagccag atgttcagtg aaaacgatct gccgctcgtg cggaagcgca agacagaacc
    54241 atgattccgt attacgcgtc acgcccggcg catagcgatg acgtaaatgt gaaaaaattt
    54301 caaactctac cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca acatcaatgg
    54361 tgacctcttc ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc
    54421 tgccggttac cgactgccag aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt
    54481 tcgtatcttg tgcgtagatg accactaaga tcgaaacggg acgcttatac actttatcct
    54541 tcacgctgcc tcttcgttga ctgccttcgc tcatcccatt cacatagtta tctatgctca
    54601 tgggagttca ctcagttgcc gcctcgatgc aacgcgaatg atttcgtgta tttgagtcat
    54661 atcagttatt ctcagccttc ttcacaacct gaatgctcag ctcagccagt gcagtcgggt
    54721 tagcaaagct cggtgcttca gtcatcagac acgctgccgc cgtggttttc gggaaggcga
    54781 taacgtcacg gatattgtcg gtgccggtca gcagcatggt cagacggtca agaccgaatg
    54841 ccagacctgc gtgcggcgga gtaccgtatt tcagagcgtc gagcaggaag ccgaatttct
    54901 cgcgctgttc ctcttcgttg atacccagaa taccaaacac cgtctgctgc atatcaccat
    54961 tatggatacg tactgaacca ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt
    55021 tcgccaccgc attttccggt gcagctttca gttctgcagc cgtcatatct ttcggtgagg
    55081 tgaacggatg gtgcattgcc gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt
    55141 cgataaccca cagcggtgcc catttgcttt cgtcggtcag accaaggtct ttacccactt
    55201 tcaggcgcag tgcacccatc gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa
    55261 tcatatcgcc atcttgcgcg gcagtacgat ccaggatgtc ttcgatgatt tctgcattaa
    55321 ggaacttcgc taccgggctg ttgatacctt ccagaccttt cgcgcgttcg ttaactttga
    55381 tgtaagccag acctttcgcg ccgtagattt taacgaagtt accgtattcg tcgatctgct
    55441 tacgggtcag cgatgcgccg cccggaacgc gcagagccgc tacgcgacct ttcggatcgt
    55501 tcgccggacc tgcaaataca gcaaactcaa cagatttcag cagatcagca acgtcagtca
    55561 gttccatcgg gttacgcaga tccggtttat cagaaccata acggcgttct gcttccgcaa
    55621 aggtcattac cgggaaatcg cccagatcca cacccttcac ttccagccac agatgacgca
    55681 ccagcgcttc catcacttca cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga
    55741 tctgagtaaa ttcaggctga cggtcagcac gcaggtcttc gtcacggaag catttaacga
    55801 tctgatagta acggtcaaaa ccggacatca tcagcagctg tttgaacaac tgcggggatt
    55861 gcggcagtgc gtagaattta cctttgtgca cacgagaagg caccaggtag tcacgcgcgc
    55921 cttccggcgt ggctttggtc agcatcggag tttcgatgtc gaggaagccg tggtcatcca
    55981 taaaacggcg caccaggctg gtgattttag cgcgggtttt caggcgctga gccatttccg
    56041 gacgacgcag gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg acgtggttag
    56101 agtcaagcgg cagaacatct gcgcggttga tgatagtcag cgaggacgcc agcacttcga
    56161 tttcgccggt cgccatatcg cggttaatat ttttttcgtc acgcgcacgt acggtgcccg
    56221 tgacctgaat gcagaactca ttacgcagtt cagaggccag ctttaacgcg tccgcacgat
    56281 ccggatcgaa aaatacctgc acgatacctt cgcggtcgcg catatcgatg aagatcaggc
    56341 taccaagatc acgacgacgg ttgacccaac cacacagagt cacctgctgc cccacgtggg
    56401 acaaacggag ctgtccacaa tattctgtac gcatgagata tcccttaact tagctgccgg
    56461 cggatgcccc ctgctgcgca ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa
    56521 tgaaaaaagg cggctattat actggaaatt ctgccgcacc gtaagagcct ggcccgcgct
    56581 ggaacgcctc gttaccactt tatatcgggc ctgaaatcag actctacgcc agtttgctat
    56641 aaaggtgttg cccgaactca taaaaattaa caaaatttgt cgttccgcca tcggctaatc
    56701 gcattaaggt gtgaggcacg attttgtttt ttcaggagtc attatgcttg aacttaatgc
    56761 taaaaccact gcgctggtgg tgatcgattt acaagaaggc atcttacctt ttgccggtgg
    56821 tccacatact gccgatgagg tagttaatcg cgccgggaag ctggcggcga aatttcgcgc
    56881 cagcggtcag cccgtgtttc tggtgcgcgt tggctggtct gccgattacg ctgaagcatt
    56941 aaaacagccg gtcgatgcgc cctcaccggc taaagtgttg cccgaaaatt ggtggcaaca
    57001 tcctgctgca ttaggtgcaa ccgacagcga tatcgaaatc atcaaacgtc aatggggtgc
    57061 gttttacggt acggatctgg agttgcaatt acgccgccga ggtatcgata caatagtgtt
    57121 atgtgggatc tcgaccaata tcggtgttga atccaccgcc cgcaatgcct gggaacttgg
    57181 ctttaatctg gtgattgccg aagacgcctg tagtgccgcc agcgccgagc agcacaataa
    57241 cagtatcaac catatctacc cgcgcatcgc ccgtgtgcgt agcgtggaag agatcctcaa
    57301 cgcgttatga tttacatcgg tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg
    57361 atcaccagcc ttgaagagta tgcccgccac tttaactgcg tggagggcaa caccacgctt
    57421 tacgccctgc cgaaacccga ggttgtcctg cgctggcgtg agcagaccac agatgacttc
    57481 cgcttctgtt ttaagtttcc ggcgaccatt tcgcatcagg cagcattacg gcattgcgat
    57541 gatttagtga ctgaattttt gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac
    57601 tggctgcaac tgcctgccac attcggccca cgggagctgc ctgcgctttg gcattttctc
    57661 gattctcttc ctggcgaatt taattatggc gtggaagtcc gccatccaca gtttttcgcc
    57721 aaaggagaag aggaacaaac gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg
    57781 attttagaca gccgcccggt tcatgcagca cgtccacaca gtgaagctat tcgcgacgct
    57841 caacgaaaaa aacctaaagt tccggtacat gctgtactga cggcgacaaa tccgctgatc
    57901 cgttttatcg gtagtgatga tatgacgcaa aaccgggaat tatttcaggt ctggttacaa
    57961 aaattagcgc agtggcatca gaccactacg ccttatcttt ttttacatac gccagacatt
    58021 gcccaggcac cggaactggt acataccctg tgggaagact tacgtaaaac gcttccagag
    58081 atcggagcag ttccggctat tccacagcaa tcttctcttt tctgaatttg ccacctatca
    58141 tagacaggtg ccatcggcca ttttaaaggg agtttgtatg gtaagcgcgc tgtatgccgt
    58201 tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta
    58261 ccgcgttgcc tatggcgacg gcggttttag cgaactgcaa agcgctattc gcattcatgg
    58321 taacgcggtg gaatatattc ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg
    58381 cgcagaaacc tggatggtgc atatttgcgg catcgttttg cttgctggtc gtctgatgca
    58441 ttattacggt tttcatcacc gtctgttccg ctggcgacgt tccggcatga gcgccacctg
    58501 gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat atgccctggg agttggtttt
    58561 ctccctgcgt tagcgcacaa tacgccactt tctttttccc ggatttttac gttatgtctc
    58621 accgcgacac gctattttct gcccctatcg ccagactggg cgactggacc tttgatgaac
    58681 gggtagctga agtcttcccg gatatgatcc agcgttccgt acccggctat tccaatatta
    58741 tttccatgat tggtatgtta gccgagcgct tcgttcaacc tggtacgcag gtttacgatc
    58801 tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg caacattcat catgataatt
    58861 gcaaaattat tgccatcgac aactccccgg cgatgattga acgctgccgt cgtcatattg
    58921 acgcctataa agcccctacg ccagtagacg tcattgaagg tgatattcgc gatatcgcca
    58981 ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca attcctggaa ccttccgagc
    59041 gccaggcatt actggataaa atttatcaag gactgaaccc gggcggcgcg ctggtgcttt
    59101 cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact gttgttcaac atgcaccacg
    59161 actttaaacg tgccaacggt tacagcgaac tggagatcag ccagaagcgc agcatgctgg
    59221 aaaacgtgat gctgaccgat tccgtggaaa cccataaagc acgcctgcat aaagccggtt
    59281 ttgagcatag tgagctgtgg ttccagtgct ttaactttgg ttcactggtg gcattaaaag
    59341 cagaggacgc tgcatgatcg actttggtaa cttttattct ctgattgcca aaaatcatct
    59401 ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac tggcagcgcg agcagcagca
    59461 cgggttgttt aagcagtggt caaatgcggt ggagtttcta cctgaaatta aaccgtatcg
    59521 tcttgattta ttgcatagcg ttacggcaga aagcgaagag ccactgagcg cagggcaaat
    59581 taaacgcatt gaaacgctga tgcgcaacct gatgccgtgg cgcaaagggc cgttctcact
    59641 gtatggcgtc aacatcgata ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc
    59701 ccatctttct gatttaaccg gacgcaccat tctggatgtc ggctgcggca gcggttatca
    59761 catgtggcgc atgattggcg caggcgcgca cctcgcggtg gggatcgacc ctacgcaact
    59821 ttttctgtgc cagtttgaag cggtgcgtaa actactgggt aatgatcagc gcgcgcattt
    59881 gttaccgtta ggtattgaac aacttccggc actgaaagcc tttgataccg tcttttcgat
    59941 gggcgtgctt taccatcgcc gttcaccgct ggagcatctc tggcagttga aagatcaact
    60001 ggtcaatgaa ggtgaactgg tgctggaaac gctggttatt gatggtgatg agaacacggt
    60061 gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct
    60121 ggcgctgaaa aactggctga agaagtgtgg ttttgtagat atccgcattg cagatgtgag
    60181 cgttaccact acagaagagc aacgacgcac cgaatggatg gttactgaat cgctggccga
    60241 tttcctcgac ccacatgatc cgagtaagac agttgaaggt tatccggcac ctaaacgtgc
    60301 ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc
    60361 cttatccggc ctacaaaacc ttgctaattc aatatattgc agggactatg taggcctgat
    60421 aagcatagcg catcaggcag ctttacgttt gcataacctc agcgcccgtt tccgggcgct
    60481 attcacttct tatgcctgaa tagctggctg atcaaacgcc gttaacttcg gcgcattgcc
    60541 cgtatatttt tcgatataca ccagcgcaga gttaccggca caggcatttg ccagctgcga
    60601 gctggggata tccgccgtta acacgttcgc actgccgttt ttacacaagc cattttccag
    60661 atctggccat gcaccttcat gcacgcatac cacgcctttt ttgatcccgt cagtcaccac
    60721 cgcgcctgtc agaatctgac cacgtttgtt ccacacacgc accagatcac cattcgcaat
    60781 accaaagcga gcagcatctt cggtgtgaat agtgattggt tcacgatctg caaccgcata
    60841 ttttttacgc agttccgcat agttaagctg actatgtaaa cggtgtgccg gatgtgcggt
    60901 cagaagctgc aactgcttct cgtcggcggt acccttccac tcatcaggcg caagccaggt
    60961 tgggtgtgcc gggcaatcct tatagccaaa tttttccagc gttttggagt aaatctcaat
    61021 tttgccgctt ggcgtaccca gcgcattttt caccggatcg gcgcggaaat caccataacg
    61081 aacgtactgt tcgttcttct cgctgcggcg catttcgatc agtttatttt gctgccagaa
    61141 ggcattaaac atcggcatag tcacgcgttg cgcacgggca cctttctgag cggcatcata
    61201 gaaaaatttc agccacgcca tttcatcttt accttcggta tagatctctt ttccgccagg
    61261 tttcagtaat tccgcaagat cggcaaacac atcaaaatcg tttcgcgctt caaattgcgg
    61321 agcgacagcc tgcttcatcg gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa
    61381 gtcattgcgc tcaaacgatg tggtgatcgg taatacgata tctgcatgtt tagcagctgc
    61441 ggtccagtag cattcagaaa cgacgatcat ctccggtttc tgccatgctt taatcagacg
    61501 attagtatcc tggtgatggg taaagttacc gccacccgcc caccagatca ttttgatatt
    61561 cggatacgtt tgctctttac cgttatgctg atatttgccg ccaggatttt ccagcgcatc
    61621 gacgatacgg gcaacaggaa tcgccgtcat tccgccatca tcagcggcct cgctggcctg
    61681 tccggcaatc gccgcagaca tttccggcag cacgccgcca acacgtgtcg ggttgccacc
    61741 attggagtaa tgataagaga aaccaaaacc gccgcccggt gtaccaatct gccctaacat
    61801 cgccgccagc gtcaccagca tccagtgttt ctgttcacca tattgctggc gctgaattcc
    61861 ccagcctgcc atcagcattg tgcgattcgc cgccatcagt tcagcgagtt tgactatctg
    61921 cgcttccggt acgccagtaa tttccgccgc ccaggccgcg ctcttcggag tattatcgct
    61981 cttacctgtc agatattctt caaactgcgg atacccggtg gtgtattttt caaggaacac
    62041 tttatcgtgt ttgccttgtg tcatcagggt atgcgcaatc cctaacatca gtgccacgtc
    62101 ggtgcccata ttcggcgcga tccaggtggc attatcgcca aagaactcaa tggtttcgga
    62161 gcggatagga tcaatggcaa tcactggttt gccagatttt ttcagctgat ggaagtattc
    62221 cagcccttgc tcatcggtac tgctccaggc aatttttaag gtattcagcg ggttcatccc
    62281 ccacagcacc acaacctggc tgttttccag aatcagcggc caggaggtct gctgttcata
    62341 cacctctaca gaaccgacca catgcggcat gatcacctgt gctgccccgg ttgaataatc
    62401 gccgctatgc ccggaataac cgcccgcgag gttcatataa cgttgcagta aggtttgcgc
    62461 tttatgcaac acgccagaag agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc
    62521 gttagcttta cgaatacgat cgtgttgctc atgaatcagt tttaatgctt gttcccagct
    62581 cacctgtaca taggtatctt cgcctcgacc tttcgccggt tgcagtggat tatcgagata
    62641 gctttttctc accatcggat gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt
    62701 agactgtaag gagttcggta tggttttcgc cagcgcgcct gtcgaagaaa caatcttgcc
    62761 gtccttcact tctacgttca tcgctcccca gcgtcccgcg gtgaggattt taccgccctt
    62821 ctcttctgcc catgcgggta gcggtgctgc cgatgtcacc accagcgctc cagcggcaat
    62881 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
    62941 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
    63001 cagttctttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
    63061 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
    63121 aagtttttca gcgtattgcc atagcggttt acggtccgct aatgcgggat cggtaagcgc
    63181 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
    63241 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
    63301 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
    63361 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
    63421 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggt
    63481 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
    63541 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
    63601 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
    63661 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
    63721 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
    63781 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatcggc
    63841 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
    63901 aggtttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
    63961 tttttgcgcc agcaatagcc cgccgccacc aaccacaacc gctatcagca aaaacaataa
    64021 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accgaaatca
    64081 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
    64141 aataatcgat aaagaatatg atttattaca atgtaatcat taatt
//
LOCUS       DAZFZC010000017        60513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFZC010000017 DAZFZC010000000
VERSION     DAZFZC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.017"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..127
                     /locus_tag="ACHKXP_001261"
                     /pseudo
     CDS             6..127
                     /locus_tag="ACHKXP_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            189..887
                     /gene="nleD"
                     /locus_tag="ACHKXP_001262"
     CDS             189..887
                     /gene="nleD"
                     /locus_tag="ACHKXP_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO7646316.1"
                     /translation="MRPTSLNLVLHQSSTSSSMSDTDIESLVKASSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVMAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHAIIVFHELLHVFHNLNGERLKVES
                     SRPESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSLGFQQVRLHPLL"
     gene            complement(984..1076)
                     /locus_tag="ACHKXP_001263"
                     /pseudo
     CDS             complement(984..1076)
                     /locus_tag="ACHKXP_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1394..1870)
                     /gene="ybhB"
                     /locus_tag="ACHKXP_001264"
     CDS             complement(1394..1870)
                     /gene="ybhB"
                     /locus_tag="ACHKXP_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO7646317.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1929..3218)
                     /gene="bioA"
                     /locus_tag="ACHKXP_001265"
     CDS             complement(1929..3218)
                     /gene="bioA"
                     /locus_tag="ACHKXP_001265"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO7646318.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYHHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANTSLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3305..4345
                     /gene="bioB"
                     /locus_tag="ACHKXP_001266"
     CDS             3305..4345
                     /gene="bioB"
                     /locus_tag="ACHKXP_001266"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO7646319.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4342..5496
                     /gene="bioF"
                     /locus_tag="ACHKXP_001267"
     CDS             4342..5496
                     /gene="bioF"
                     /locus_tag="ACHKXP_001267"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO7646320.1"
                     /translation="MSWQDKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5483..6238
                     /gene="bioC"
                     /locus_tag="ACHKXP_001268"
     CDS             5483..6238
                     /gene="bioC"
                     /locus_tag="ACHKXP_001268"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO7646321.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6231..6908
                     /gene="bioD"
                     /locus_tag="ACHKXP_001269"
     CDS             6231..6908
                     /gene="bioD"
                     /locus_tag="ACHKXP_001269"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7646322.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7487..9508
                     /gene="uvrB"
                     /locus_tag="ACHKXP_001270"
     CDS             7487..9508
                     /gene="uvrB"
                     /locus_tag="ACHKXP_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO7646323.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9700..10608)
                     /gene="yvcK"
                     /locus_tag="ACHKXP_001271"
     CDS             complement(9700..10608)
                     /gene="yvcK"
                     /locus_tag="ACHKXP_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO7646324.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10873..11017
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11005..11994
                     /gene="moaA"
                     /locus_tag="ACHKXP_001272"
     CDS             11005..11994
                     /gene="moaA"
                     /locus_tag="ACHKXP_001272"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO7646325.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVASWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12016..12528
                     /gene="moaB"
                     /locus_tag="ACHKXP_001273"
     CDS             12016..12528
                     /gene="moaB"
                     /locus_tag="ACHKXP_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO7646326.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12531..13016
                     /gene="moaC"
                     /locus_tag="ACHKXP_001274"
     CDS             12531..13016
                     /gene="moaC"
                     /locus_tag="ACHKXP_001274"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO7646327.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13009..13254
                     /gene="moaD"
                     /locus_tag="ACHKXP_001275"
     CDS             13009..13254
                     /gene="moaD"
                     /locus_tag="ACHKXP_001275"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO7646328.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13256..13708
                     /gene="moaE"
                     /locus_tag="ACHKXP_001276"
     CDS             13256..13708
                     /gene="moaE"
                     /locus_tag="ACHKXP_001276"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO7646329.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSE
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13844..14548
                     /gene="ybhL"
                     /locus_tag="ACHKXP_001277"
     CDS             13844..14548
                     /gene="ybhL"
                     /locus_tag="ACHKXP_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO7646330.1"
                     /translation="MDRFPRSDSIVQSRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14753..15466
                     /gene="ybhM"
                     /locus_tag="ACHKXP_001278"
     CDS             14753..15466
                     /gene="ybhM"
                     /locus_tag="ACHKXP_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7646331.1"
                     /translation="MESYSKNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSLPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15502..16458)
                     /gene="ybhN"
                     /locus_tag="ACHKXP_001279"
     CDS             complement(15502..16458)
                     /gene="ybhN"
                     /locus_tag="ACHKXP_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO7646332.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16458..17699)
                     /gene="clsB"
                     /locus_tag="ACHKXP_001280"
     CDS             complement(16458..17699)
                     /gene="clsB"
                     /locus_tag="ACHKXP_001280"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO7646333.1"
                     /translation="MKCSWREGNKIELLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17696..18457)
                     /gene="ybhP"
                     /locus_tag="ACHKXP_001281"
     CDS             complement(17696..18457)
                     /gene="ybhP"
                     /locus_tag="ACHKXP_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7646334.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLQTWRHLSDHAPLSAEIHL"
     gene            18590..19000
                     /gene="ybhQ"
                     /locus_tag="ACHKXP_001282"
     CDS             18590..19000
                     /gene="ybhQ"
                     /locus_tag="ACHKXP_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO7646335.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18962..20068)
                     /gene="ybhR"
                     /locus_tag="ACHKXP_001283"
     CDS             complement(18962..20068)
                     /gene="ybhR"
                     /locus_tag="ACHKXP_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7646336.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20079..21212)
                     /gene="ybhS"
                     /locus_tag="ACHKXP_001284"
     CDS             complement(20079..21212)
                     /gene="ybhS"
                     /locus_tag="ACHKXP_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7646337.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21205..22941)
                     /gene="ybhF"
                     /locus_tag="ACHKXP_001285"
     CDS             complement(21205..22941)
                     /gene="ybhF"
                     /locus_tag="ACHKXP_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7646338.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFGPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22934..23929)
                     /gene="hlyD"
                     /locus_tag="ACHKXP_001286"
     CDS             complement(22934..23929)
                     /gene="hlyD"
                     /locus_tag="ACHKXP_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO7646339.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23932..24603)
                     /gene="cecR"
                     /locus_tag="ACHKXP_001287"
     CDS             complement(23932..24603)
                     /gene="cecR"
                     /locus_tag="ACHKXP_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO7646340.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24832..26196
                     /gene="rhlE"
                     /locus_tag="ACHKXP_001288"
     CDS             24832..26196
                     /gene="rhlE"
                     /locus_tag="ACHKXP_001288"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO7646341.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26428..26910)
                     /gene="ybiA"
                     /locus_tag="ACHKXP_001289"
     CDS             complement(26428..26910)
                     /gene="ybiA"
                     /locus_tag="ACHKXP_001289"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO7646342.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGHGKGKNRLGYLLMELREQLAIEK
                     "
     gene            27030..29180
                     /gene="dinG"
                     /locus_tag="ACHKXP_001290"
     CDS             27030..29180
                     /gene="dinG"
                     /locus_tag="ACHKXP_001290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO7646343.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29208..30170
                     /gene="ybiB"
                     /locus_tag="ACHKXP_001291"
     CDS             29208..30170
                     /gene="ybiB"
                     /locus_tag="ACHKXP_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO7646344.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30311..31396
                     /gene="hcxB"
                     /locus_tag="ACHKXP_001292"
     CDS             30311..31396
                     /gene="hcxB"
                     /locus_tag="ACHKXP_001292"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO7646345.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31463..31522
                     /gene="ybiE"
                     /locus_tag="ACHKXP_001293"
     CDS             31463..31522
                     /gene="ybiE"
                     /locus_tag="ACHKXP_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO7646346.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31625..31885)
                     /gene="ybiJ"
                     /locus_tag="ACHKXP_001294"
     CDS             complement(31625..31885)
                     /gene="ybiJ"
                     /locus_tag="ACHKXP_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO7646347.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(32150..32416)
                     /gene="ybiI"
                     /locus_tag="ACHKXP_001295"
     CDS             complement(32150..32416)
                     /gene="ybiI"
                     /locus_tag="ACHKXP_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO7646348.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32490..33167)
                     /gene="ybiX"
                     /locus_tag="ACHKXP_001296"
     CDS             complement(32490..33167)
                     /gene="ybiX"
                     /locus_tag="ACHKXP_001296"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO7646349.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSTTLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33209..35491)
                     /gene="fiu"
                     /locus_tag="ACHKXP_001297"
     CDS             complement(33209..35491)
                     /gene="fiu"
                     /locus_tag="ACHKXP_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO7646350.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAR
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(35756..36016)
                     /gene="mcbA"
                     /locus_tag="ACHKXP_001298"
     CDS             complement(35756..36016)
                     /gene="mcbA"
                     /locus_tag="ACHKXP_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO7646351.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36292..37218
                     /gene="rlmF"
                     /locus_tag="ACHKXP_001299"
     CDS             36292..37218
                     /gene="rlmF"
                     /locus_tag="ACHKXP_001299"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO7646352.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(37215..39440)
                     /gene="ybiO"
                     /locus_tag="ACHKXP_001300"
     CDS             complement(37215..39440)
                     /gene="ybiO"
                     /locus_tag="ACHKXP_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO7646353.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(39701..40423)
                     /gene="glnQ"
                     /locus_tag="ACHKXP_001301"
     CDS             complement(39701..40423)
                     /gene="glnQ"
                     /locus_tag="ACHKXP_001301"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO7646354.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(40420..41079)
                     /gene="glnP"
                     /locus_tag="ACHKXP_001302"
     CDS             complement(40420..41079)
                     /gene="glnP"
                     /locus_tag="ACHKXP_001302"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO7646355.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(41218..41964)
                     /gene="glnH"
                     /locus_tag="ACHKXP_001303"
     CDS             complement(41218..41964)
                     /gene="glnH"
                     /locus_tag="ACHKXP_001303"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO7646356.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(42368..42871)
                     /gene="dps"
                     /locus_tag="ACHKXP_001304"
                     /gene_synonym="pexB"
     CDS             complement(42368..42871)
                     /gene="dps"
                     /locus_tag="ACHKXP_001304"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO7646357.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(43170..44057)
                     /gene="rhtA"
                     /locus_tag="ACHKXP_001305"
     CDS             complement(43170..44057)
                     /gene="rhtA"
                     /locus_tag="ACHKXP_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO7646358.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            44292..44357
                     /locus_tag="ACHKXP_001306"
     CDS             44292..44357
                     /locus_tag="ACHKXP_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646359.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            44410..44925
                     /gene="ompX"
                     /locus_tag="ACHKXP_001307"
     CDS             44410..44925
                     /gene="ompX"
                     /locus_tag="ACHKXP_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO7646360.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(44974..46557)
                     /gene="opgE"
                     /locus_tag="ACHKXP_001308"
     CDS             complement(44974..46557)
                     /gene="opgE"
                     /locus_tag="ACHKXP_001308"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7646361.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(46829..46957)
                     /gene="mntS"
                     /locus_tag="ACHKXP_001309"
     CDS             complement(46829..46957)
                     /gene="mntS"
                     /locus_tag="ACHKXP_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO7646362.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            47143..47610
                     /gene="mntR"
                     /locus_tag="ACHKXP_001310"
     CDS             47143..47610
                     /gene="mntR"
                     /locus_tag="ACHKXP_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO7646363.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            47607..48725
                     /gene="ybiR"
                     /locus_tag="ACHKXP_001311"
     CDS             47607..48725
                     /gene="ybiR"
                     /locus_tag="ACHKXP_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO7646364.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(48784..49704)
                     /gene="ldtB"
                     /locus_tag="ACHKXP_001312"
     CDS             complement(48784..49704)
                     /gene="ldtB"
                     /locus_tag="ACHKXP_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7646365.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            49923..51515
                     /gene="ybiT"
                     /locus_tag="ACHKXP_001313"
     CDS             49923..51515
                     /gene="ybiT"
                     /locus_tag="ACHKXP_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO7646366.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(51756..53021)
                     /gene="ybiU"
                     /locus_tag="ACHKXP_001314"
     CDS             complement(51756..53021)
                     /gene="ybiU"
                     /locus_tag="ACHKXP_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO7646367.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(53173..53988)
                     /gene="ybiV"
                     /locus_tag="ACHKXP_001315"
     CDS             complement(53173..53988)
                     /gene="ybiV"
                     /locus_tag="ACHKXP_001315"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO7646368.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(54134..56566)
                     /gene="ybiW"
                     /locus_tag="ACHKXP_001316"
     CDS             complement(54134..56566)
                     /gene="ybiW"
                     /locus_tag="ACHKXP_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO7646369.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFEGLTHEQLRQRLINGAPKYGNDDDT
                     VDMLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(56572..57471)
                     /gene="ybiY"
                     /locus_tag="ACHKXP_001317"
     CDS             complement(56572..57471)
                     /gene="ybiY"
                     /locus_tag="ACHKXP_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO7646370.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPDVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            57602..58264
                     /gene="fsa"
                     /locus_tag="ACHKXP_001318"
     CDS             57602..58264
                     /gene="fsa"
                     /locus_tag="ACHKXP_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7646371.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLTGCESITLPLDVAHQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(58443..59192)
                     /gene="moeB"
                     /locus_tag="ACHKXP_001319"
     CDS             complement(58443..59192)
                     /gene="moeB"
                     /locus_tag="ACHKXP_001319"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO7646372.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGTLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENSLTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCVQ"
     gene            complement(59192..60427)
                     /gene="moeA"
                     /locus_tag="ACHKXP_001320"
     CDS             complement(59192..60427)
                     /gene="moeA"
                     /locus_tag="ACHKXP_001320"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO7646373.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
BASE COUNT        14470 a        15490 c        16145 g        14408 t
ORIGIN      
        1 ggtaacaaat tgtgtggttg gcaattcttg gcggatcacg gaaatgggca atgtcgctga
       61 ggactggcaa atggctatga tccgctttat tatcgggttt ggtgaccgcc ttgacggtca
      121 cttttaaaaa aatcattttc acagagtggt aaacaggctg ctaataagta gcattctcag
      181 gagtcctgat gcgccctacg tccctcaact tggtattaca tcagtcatca acgtcgagct
      241 caatgtcaga tacagatatc gagtctcttg taaaagcatc gagcgttcaa tggataaaaa
      301 ataatccgca acttcgtttc caggggactg atcataatat atatcagcag attgaagcag
      361 cactcgataa gattggctct acagagacag ggcgtgtact cctgaatgct attgaatcaa
      421 tatcccgact taaatcagaa acagtggtaa tacacctcaa ctcttccaga ctaggagtta
      481 tggcacatag agatatagat gctgagaacc atcgggggac tggttccgat tttcactgta
      541 atctgaatgc agttgaatat ccctgtgggg aggggattag cgtggtggac tttcatgcga
      601 ttattgtttt tcatgagttg ctccatgttt tccacaattt aaatggggag cgtttgaaag
      661 ttgagagttc ccgaccagaa tcacaaaaat actctccact tttactcgaa gaagccagga
      721 ctgttgggtt gggggctttt tcagaggagg tgctttcaga aaataaattc cgcgaagaga
      781 ttgggatgcc ccgtagaacc tcctacccgc acgactcagc tcttattcat gatgacaata
      841 cagtgagtct gggattccaa caggtaagac tgcatccatt gctttagctg tgtttcttgt
      901 gctgaatata tattggtggg gcaaacggag gcacacaaaa ctttgcactg gattgcaagg
      961 ctttgtgcta ttccgatagt ggttcaggtg gagtacccca ccttttcatc aagccagccc
     1021 acccaccact gcatcatttc tctgcgctta tagagatact gagcatggtt gtaaattcag
     1081 caaacgacgt aatgtgtttg acaaaaaatt agcgcaagaa gacaaaaatc accttgcgct
     1141 aatgctctgt tacaggtcac taataccatc taagtagttg attcatagtg actggatatg
     1201 ttgtgttttg tagcattatg cagtctattt tttagactga atcaattgta agccgttgat
     1261 attaattgtt tttaccgttt catattcaac ttttttatac taacttgagc gaaacgggaa
     1321 ggtaaaaaga caaaaagttg tttttaatac ctttaagtga taccagatgg cattgcgcca
     1381 tctggcagag tgattaacta aacatcgcag taatcgaggc gcttgccaga gagtggaaat
     1441 gaacgttaaa cccgaccatc gcgccgctgg caccttcatc gacatcaata cgttctatat
     1501 ccagcgcgtg aacggtaaaa atgtagcgat gagtttcgcc tttcggcggt gctgcgccat
     1561 cgtacccggt tttaccaaag tcggtacgcg tctgcaaaac gccgtctggc attgctacca
     1621 gaccagagcc aaacccttgc ggtaatacgc gggtatcagc gggtaagtta acaactaccc
     1681 agtgccacca gccggagccg gttggcgcat ccgggtcgta gcaggtgaca acaaaacttt
     1741 tcgttcccgc aggaacatca tcccacgcca gatgcggtga aatattatcg ccatcgtaac
     1801 ccatgccgtt aaagacatga cgatgcggca atttatcgcc atcgcgcaga tcgttactga
     1861 tgagtttcat gaaccctcct ttcttgtttg cagaaagtgt agccagaaac cctcacgcgg
     1921 acttctcgtt attggcaaaa aaatgtttca tcctgtaccg cgcggttaac cgctgcggtc
     1981 agacgctgca actgttgcgg gagaataata tagggcggca tcaggtaaat cagtttgcca
     2041 aaaggccgga tccagacacc ctgttcgaca aagaattttt gcagcgccgc catattcacc
     2101 ggatgagtgg tttcgaccac gccaatggcc cccagtacgc gcacatcggc aaccatttcg
     2161 gcatcacggg cgggggcaag ttgctcgcgc agctgtactt caatatccgc cacctgttgc
     2221 tgccagtcgc cagattcgag aatcgccagg ctggtgtttg ctgccgcgca ggccagcgga
     2281 ttgcccataa aagttggccc atgcataaag caaccggctt caccgttact gatggtttct
     2341 gcaacctcgc gcgtggtgag tgtggcggaa agggtcattg tgccgccggt taaggcttta
     2401 ccgaggcaca aaatgtccgg cgcgatttct gcatgttcac aggcaaacag tttcccggta
     2461 cgaccaaatc cagtggcgat ctcgtcggca atcagcaaga taccttcgcg atcgcatatt
     2521 ttgcggattc gttttaacca ttccggatgg tacatgcgca tcccgcctgc gccctggaca
     2581 atcggctcaa tgatcaccgc cgcgatttca tgacgatgcg ccgccatcag gcgggcaaag
     2641 cccaccatat cgcgctcatc ccattcgcca tccatacggc tttgtggggc gggagcaaac
     2701 aagttttctg gcagatagcc tttccacagg ctgtgcattg agttgtccgg atcgcacacc
     2761 gacatcgcgc caaaggtatc gccatgataa ccattgcgga aggtcagaaa acgctggcgc
     2821 gcttcgcctt tggcctgcca gtactgcaac gccattttca tcgccacttc caccgctacg
     2881 gagccggagt ccgcgagaaa aacgcattcc agcggttgcg gcgtcattgc caccagtttg
     2941 cggcatagct cgatggcggg cgcatgggtg ataccgccaa acatcacatg cgacatagca
     3001 tcaatttgcg acttcatcgc cgcattaagc tgcgggtgat gatagccgtg gatcgccgcc
     3061 caccaggacg acataccgtc aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct
     3121 tcggcgctcg cgaccggata aaccggcaga ggggaggtca tggatgtgta tgggtgccag
     3181 atatggcgtt ggtcaaaggc aagatcgtcc gttgtcataa tcgacttgta aaccaaattg
     3241 aaaagattta ggtttacaag tctacaccga attaacaaca aaaaacacgt tttggagaag
     3301 ccccatggct caccgcccac gctggacatt gtcgcaagtc acagaattat ttgaaaaacc
     3361 gttgctggat ctgctgtttg aagcgcagca ggtacatcga cagcatttcg atcctcgtca
     3421 ggtgcaggtc agcacgttgc tgtcgattaa gaccggagct tgtccggaag attgcaaata
     3481 ctgcccgcaa agctcgcgct acaaaaccgg gctggaagcc gagcggttga tggaagttga
     3541 acaggtgctg gagtcggcgc gcaaagcgaa agcggcagga tcgacgcgct tctgtatggg
     3601 cgcggcgtgg aagaatcccc acgaacgcga tatgccgtac ctggaacaaa tggtgcaggg
     3661 ggtaaaagcg atggggctgg aggcgtgtat gacgctgggc acgttgagtg aatctcaggc
     3721 gcagcgcctc gcgaacgccg ggctggatta ctacaaccac aacctggaca cctcgccgga
     3781 gttttacggc aatatcatca ccacacgcac ttatcaggaa cgcctcgata cgctggaaaa
     3841 agtgcgcgat gccgggatca aagtctgttc tggcggcatt gtgggcttag gcgaaacggt
     3901 aaaagatcgc gccggattat tgctgcaact ggcaaacctg ccgacgccgc cggaaagcgt
     3961 gccaatcaac atgctggtga aggtgaaagg cacgccgctt gccgataacg atgatgtcga
     4021 tgcctttgat tttattcgca ccattgcggt cgcgcggatc atgatgccaa cctcttacgt
     4081 gcgcctttct gccggacgcg agcagatgaa cgaacagact caggcgatgt gctttatggc
     4141 aggcgcaaac tcgattttct acggttgcaa actgctgacc acgccgaatc cggaagaaga
     4201 taaagacctg caactgttcc gcaaactggg gctaaatccg cagcaaactg ccgtgctggc
     4261 gggcgataac gaacaacagc agcgtctgga acaggcactg atgaccccgg acactgacga
     4321 atattacaac gcggcagcac tatgagctgg caggataaaa tcaacgcggc cctcgatgcg
     4381 cggcgtgctg ccgatgccct gcgtcgccgt tatccagtgg cgcaaggagc aggacgctgg
     4441 ctggtggcgg acgatcgcca gtatctgaac ttttccagta acgattattt aggtttaagc
     4501 catcatccgc aaattatccg tgcctggaag cagagtgcgg agcaatttgg cgtcggtagc
     4561 ggcggctccg gtcacgtcag cggttatagc gtggcacatc aggcgctgga agaagaactg
     4621 gccgagtggc tgggctattc gcgggcactg ctgtttatct ctggttttgc cgctaatcag
     4681 gcggtcattg ccgcgatgat ggcgaaagag gaccgtattg ttgccgaccg gcttagccat
     4741 gcctcattgc tggaggctgc cagtttaagc ccgtcgcagc ttcgccgttt tgttcataac
     4801 gatgtcactc atctggcgcg actgcttgct tccccctgtc cggggcagca aatggtggtg
     4861 acagaaggcg tgttcagcat ggacggcgat agtgcgccac tggcggaaat ccagcaggta
     4921 acgcaacagc acaatggctg gttgatggtc gatgatgccc acggcacggg cgttatcggg
     4981 gagcaggggc gcggcagctg ctggctgcaa aaggtaaaac cagaattgct ggtagtgact
     5041 tttggcaaag gatttggcgt cagcggggca gcggtgcttt gctccagtac ggtggcggat
     5101 tatctgctgc aattcgcccg ccaccttatc tacagcacca gtatgccgcc cgctcaggcg
     5161 caggcattac gtgcgtcgct ggcggtcatt cgcagtgatg agggtgatgc acggcgcgaa
     5221 aaactggcgg cactcattac gcgttttcgt gccggagtac aggatttgcc gtttacgctt
     5281 gctgattcat gcagcgccat ccagccattg attgtcggtg ataacagccg tgcgttacaa
     5341 ctggcagaaa aactgcgcca gcaaggctgc tgggtcacgg cgattcgccc gccaaccgta
     5401 cccgctggta ctgcgcgact gcgcttaacg ctaaccgctg cgcatgaaat gcaggatatc
     5461 gaccgtctgc tggaggtgct gcatggcaac ggttaataaa caagccattg cagcggcatt
     5521 tggtcgggca gccgcacact atgagcaaca tgcagatcta cagcgccaga gtgctgacgc
     5581 cttactggca atgcttccac agcgtaaata cacccacgta ctggacgcgg gttgtggacc
     5641 tggctggatg agccgccact ggcgggaacg tcacgcgcag gtgacggcct tagatctctc
     5701 gccgccaatg cttgttcagg cacgccagaa ggatgccgca gaccattatc tggcgggaga
     5761 tatcgaatcc ctgccgttag cgactgcgac gttcgatctt gcatggagca atctcgcagt
     5821 gcagtggtgc ggtaatttat ccacggcact ccgcgagctg tatcgggtgg tgcgccccaa
     5881 aggcgtggtc gcgtttacca cgctggtgca gggatcgtta cccgaactgc atcaggcgtg
     5941 gcaggcggtg gacgagcgtc cgcatgctaa tcgcttttta ccgccagatg aaatcgaaca
     6001 gtcgctgaac ggcgtgcatt atcaacatca tattcagccc atcacgctgt ggtttgatga
     6061 tgcgctcagt gccatgcgtt cgctgaaagg catcggtgcc acgcatcttc atgaagggcg
     6121 cgacccgcga atattaacgc gttcgcagtt gcagcgattg caactggcct ggccgcaaca
     6181 gcaggggcga tatcctctga cgtatcatct ttttttggga gtgattgctc gtgagtaaac
     6241 gttattttgt caccggaacg gataccgaag tggggaaaac tgtcgccagt tgtgcacttt
     6301 tacaagccgc aaaggcagca ggctaccgga cggcaggtta taaaccggtc gcctctggca
     6361 gcgaaaagac cccggaaggt ttacgcaata gcgacgcgct ggcgttacag cgcaacagca
     6421 gcctgcagct ggattacgca acagtaaatc cttacacctt cgcagaaccc acttcgccgc
     6481 acatcatcag cgcgcaagag ggcagaccga tagaatcatt ggtaatgagc gccggattac
     6541 gcgcgcttga acaacaggct gactgggtgt tagtggaagg tgctggcggc tggtttacgc
     6601 cgctttctga cactttcact tttgcagatt gggtaacaca ggaacaactg ccggtgatac
     6661 tggtagttgg tgtgaaactc ggctgtatta atcacgcgat gttgactgca caggtaatac
     6721 aacacgccgg actgactctg gcgggttggg tggcgaacga tgttacgcct ccgggaaaac
     6781 gtcacgctga atatatgacc acgctcaccc gcatgattcc cgcgccgctg ctgggagaga
     6841 tcccctggct tgcagaaaat ccagaaaatg cggcaaccgg aaagtacata aaccttgcct
     6901 tgttgtagcc attctgtatt tggttaaatt gcgagcgaga tcgcgtcttc gattgactgc
     6961 aatttaacca attaaattct aaaataatca cgaaaaaaat tttacttccg cctcatgcgg
     7021 cgaatgtggg aattgcccag gcggcggggg ataggggctg cagacagtta tccactattc
     7081 ctgtggataa ccatgtgtat tagagttaga aaacacgagg caagcgagag aatacgcggc
     7141 ttgcacgcga attggcgtta aagacggctc aaagaaatat cttttatttt ttaactggtt
     7201 agataaatgc aatggcagtc actgaacagg catctcttgc cataaaactg tcatcactca
     7261 tcttgacaaa tgttaaaaaa gccgttgctt tggggataac ccggtaaggc cggagtttta
     7321 tctcgccaca gagtaaattt tgctcatgat tgacagcgga gtttacgctg tatcagaaat
     7381 attatggtga tgaactgttt ttttatccag tataatttgt tggcataatt aagtacgacg
     7441 agtaaaatta catacctgcc cgcccaactc cttcaggtag cgactcatga gtaaaccgtt
     7501 caaactgaat tccgctttta aaccttctgg cgatcagcca gaggcgattc gacgtctcga
     7561 agaggggctg gaagatggcc tggcgcacca gacgttactt ggcgtgactg gctcagggaa
     7621 aaccttcacc attgccaatg tcattgctga ccttcagcgc ccaaccatgg tacttgcgcc
     7681 caacaaaacg ctggcggccc agctgtatgg cgaaatgaaa gagttcttcc cggaaaacgc
     7741 ggtggaatat ttcgtttcct actacgacta ctatcagccg gaagcctatg taccgagttc
     7801 cgacactttc attgagaaag atgcctcggt taacgaacat attgagcaga tgcgtttgtc
     7861 cgccaccaaa gcgatgctgg agcggcgtga tgtggttgtg gtggcgtctg tttccgcgat
     7921 ttatggtctg ggcgatcctg atttatatct caagatgatg ctccatctca cggtcggtat
     7981 gattatcgat cagcgcgcga ttctgcgccg actggcggag ctgcaatacg ctcgtaatga
     8041 tcaagcattc cagcgtggta ctttccgcgt tcgtggcgag gtgatagata tcttcccggc
     8101 agaatcggat gacattgcac ttcgcgtgga actgtttgac gaggaagtgg aacgattgtc
     8161 gttatttgac ccgctgaccg ggcagattgt ttccactatt ccacgtttta ccatctaccc
     8221 gaaaacgcac tacgtcacac cgcgcgagcg catcgtacag gcgatggagg agatcaaaga
     8281 agagctggcc gccagacgca aagtgctgtt ggaaaacaac aaactgctgg aagagcagcg
     8341 gctgacccag cgtacccagt ttgatctgga gatgatgaac gagctgggct actgttcggg
     8401 gattgaaaac tactcgcgct tcctctccgg tcgtggaccg ggtgagccac cgccgacgct
     8461 gtttgattac ctgcctgccg atgggctgct ggtcgtcgat gaatctcacg tcaccattcc
     8521 acaaattggc ggcatgtatc gcggtgaccg ggcgcgtaaa gagacactgg tggagtacgg
     8581 cttccgcctg ccatcagcgc tggataaccg tccgcttaag tttgaagagt tcgaagcatt
     8641 agcgccgcaa accatctatg tttcggcgac gccgggtaat tacgagctgg aaaaatccgg
     8701 cggcgatgtg gtggatcagg tggtgcgtcc aaccggattg cttgacccga ttatcgaagt
     8761 gcggccggtg gcgacacagg ttgatgatct tctttcggag attcgtcagc gagcggcaat
     8821 taacgaacgc gtactggtca ccacactgac caagcggatg gcggaagatc ttaccgaata
     8881 tctcgaagaa catggcgagc gcgtgcgtta tcttcactca gatatcgaca ccgtcgaacg
     8941 tatggagatt atccgcgact tgcgtctggg tgagttcgac gtgctggtag ggatcaactt
     9001 actgcgcgaa ggtctggata tgccggaagt gtcgctggtg gcgatcctcg acgctgacaa
     9061 agaaggcttc ctgcgttccg aacgttcgtt gatccagacc attggtcgtg cggcacgtaa
     9121 cgttaacggt aaagcgattc tctacggcga taagatcacc ccatcaatgg cgaaagcgat
     9181 tggcgaaacc gaacgtcgcc gtgagaaaca gcagaagtac aacgaggaac acggaattac
     9241 gccgcaaggc ttgaacaaga aagtggtcga tatcctggcg ctggggcaga acattgccaa
     9301 aaccaaagcg aagggcagag gaaaatcgcg cccgattgtt gagccggata atgtgccgat
     9361 ggatatgtcg cctaaagcgt tgcagcagaa aatccatgag ctggaagggt tgatgatgca
     9421 acacgcgcag aatctggagt tcgaagaagc ggcgcaaatt cgtgaccagt tgcatcagct
     9481 gcgtgagctg tttatcgcgg catcgtaaca ggatagcgaa gaagactgat gacaaacgga
     9541 aaacagcctg atgcgctacg cttatcaggc ctacattttc tccgcaatat attgaatttg
     9601 cgcggtttgt aggccggata aggcgttcac gccgcatccg gcataaacaa cgcgcacttt
     9661 gtcttcaata ggaagccgga attttccttc ggatttccgt taacctaaag cctgtaacgc
     9721 cttttccagc gcgttatgta acaactggcg gtcatgacga tacggaatat cgctggcctc
     9781 cagtacctcc tggatcacaa tccgctcttt caccgccgag acatccactt ttggcccgac
     9841 gatgaccgca tcaatgacct ttttaccaac atactgctcc ataattgcca gcttgctttc
     9901 cagcttcaaa ttagccgcag gtaaactcaa ctcacgcccc agattgccga tataaaccat
     9961 cggcgctggc gtgcggcgta atgcctgggc gatttccttc agcagcagaa ttggcatcag
    10021 gctggtataa aaactgccag gcccaataat gatgagatcc gcttcattga tagcgtgaac
    10081 cgcctcacgc gttgcgggta cattaggcgt taacaataac tcttgaatcg gcgtagttaa
    10141 ctggtcgata ttgacctcgc cgtaaacttc atgcccctga tcgtcaatcg ccatcagatc
    10201 aacaggatgc tctgacattg gaatcaaatg cgtatccact ttcagcagat tacgaattaa
    10261 attgatggct tccagaggcc gcacgctaag gtgatccagc gcctttaaca tcaagtttcc
    10321 gagattatga ccggaaagtt cgccattgcc accaaaacgg tattcaaaca tcgcggaggc
    10381 gacgctcggt tccgttatca gctggttgag gcagttgcgc atatcgcccc aggcaatgcc
    10441 gccttctgaa cggcgaatac gccccgtcga gccaccatta tcggtggtgg tgacgatacc
    10501 cgttaaacga gaacccaaag acgaaagtga tgagagaacg cgtcccagtc catgccctcc
    10561 gccgagagca acgacacgat caagatcagc cagcgtacga ttgcgcatat agtttcctgg
    10621 agtcagatta tccgcgctac agtagcgcaa attgcgggga aacagcaatt aacctgccaa
    10681 tattgaggat acaaactatt ttcttctacc tctaaaggac gatgcacgct atgcctccct
    10741 gatgatgtat atcaaagtaa aaccgccatt ttcccttatt ctgtagcgaa atagcacgat
    10801 catgacgcta tatacatgat tacatagcga aagtgtggat ggtaaaaatc tcatttacac
    10861 gctagtatcg gcataaccac taaacactct agcctctgca cctgggtcaa ctgatacggt
    10921 gctttggccg tgacaatgct cgtaaagatt gccaccaggg cgaaggaaga aatgacttcg
    10981 cctcccgtat ctggaaaggt gtacatggct tcacaactga ctgatgcatt tgcgcgtaag
    11041 ttttactact tgcgcctgtc gattaccgat gtgtgtaact ttcgttgcac ctactgcctg
    11101 ccggatggct acaaaccgag cggcgtcacc aataaaggct ttcttaccgt cgatgaaatt
    11161 cgccgggtta cgcgcgcctt cgccagtctg ggcaccgaaa aagtgcgcct gacaggagga
    11221 gagccgtctt tacgccgcga ctttaccgat atcatcgccg ctgtgcggga aaacgacgct
    11281 atccgccaga ttgcggtcac aaccaatggt taccgtctgg aacgcgatgt ggcgagctgg
    11341 cgcgatgcgg gacttactgg cattaacgtc agtgtcgaca gtctggacgc ccgccagttt
    11401 cacgctatta ccgggcagga taaattcaac caggtcatgg cagggattga tgctgcattt
    11461 gaggccggtt ttgagaaggt caaagtcaat accgtgctga tgcgtgatgt taatcatcac
    11521 cagctcgaca cctttctgaa ctggatccag catcgcccta tccagctgcg tttcatcgaa
    11581 ctgatggaaa cgggcgaggg cagcgagctc ttccgtaagc atcacatctc tggtcaggtt
    11641 ctgcgtgacg agctactgcg tcgcggctgg atccaccaat tacgtcaacg cagcgacggt
    11701 cctgcgcaag tcttttgcca tccagattac gccggagaga ttggccttat catgccgtat
    11761 gaaaaagact tctgcgccac ttgcaaccgc ctgcgcgttt cctccattgg taaactccat
    11821 ctctgcctgt ttggtgaagg cggcgttaac ctgcgcgatc tgctggaaga cgatacccag
    11881 caacaggcgc tggaagcgcg tatttcagcg gcgctgcggg agaagaaaca gacccatttc
    11941 ctgcatcaaa acaacaccgg tattacgcaa aacttatcgt acattggcgg ctaaaacgtc
    12001 aaaaggagag atcagatgag tcaggtaagc actgaattta tcccgacccg tattgctatt
    12061 cttacggttt ctaatcgtcg cggtgaagaa gacgatacct ccggtcacta tctgcgcgat
    12121 tcggcgcaag aagcgggcca tcacgttgtc gataaagcca ttgtgaaaga aaaccgctac
    12181 gctattcgcg ctcaggtatc tgcgtggatc gccagcgacg atgtacaagt ggtattgatt
    12241 acgggtggta ctggcctgac ggaaggtgat caggctcccg aagcattgct gccgttgttc
    12301 gaccgtgaag ttgaaggttt tggtgaagtg ttccggatgt tgtcgtttga agagattggc
    12361 acttccacgt tgcaatctcg tgcggtagcg ggcgtcgcca acaaaacgct gattttcgcc
    12421 atgccgggtt cgaccaaagc gtgccgtacc gcatgggaaa atatcatcgc gccgcagctg
    12481 gatgcccgta cgcgtccgtg taatttccat ccacatttga agaaataagt atgtcgcaac
    12541 tgacccatat caacgccgct ggcgaagcgc acatggtgga tgtctccgcc aaagcggaaa
    12601 ccgtgcgtga agcgcgggcg gaagcctttg tcaccatgcg cagcgagacg ctggcgatga
    12661 ttattgatgg tcgccaccac aaaggcgacg tatttgccac tgcgcgtatt gccggtattc
    12721 aggcggcaaa acgcacctgg gatctgatcc cgctctgtca tccgctgatg ctcagcaaag
    12781 ttgaagtcaa tttacaggcc gagccggagc acaatcgggt gcgtatagaa accttatgcc
    12841 gcctgaccgg gaaaaccggt gtcgaaatgg aagcattaac cgcggcctcc gtggcggcgc
    12901 tgaccattta tgacatgtgc aaagcggtgc aaaaagatat ggtgattggt ccggtacgtt
    12961 tgctggcgaa gagcggcggc aagtcgggtg actttaaggt ggaagcggat gattaaagtt
    13021 ctttttttcg cccaggtgcg cgagttggtg ggaacagatg caaccgaagt ggctgcggat
    13081 ttcccaactg ttgaagcgtt acgccagcac atggctgcgc agagcgatcg ctgggcgctg
    13141 gcgctggaag atggcaaatt gctggctgcc gtcaaccaga cgctggtgag ttttgaccat
    13201 ccgctgactg acggcgacga agtggctttc ttcccgccgg taaccggagg ttaagatggc
    13261 agaaaccaaa attgttgtcg gcccgcagcc attcagcgta ggagaagagt atccgtggct
    13321 ggcggagcgt gatgaagacg gtgcggtagt cacctttacc ggtaaggtgc gcaaccataa
    13381 ccttggcgac agcgtcaaag cattaaccct cgaacactat ccggggatga ctgaaaaagc
    13441 actggcagaa attgtcgatg aagcgcgtaa ccgctggccg ctggggcgcg tcacggtgat
    13501 tcaccgcatc ggggaattat ggccgggcga tgaaatcgtt tttgtcggtg tcaccagtga
    13561 gcaccgcagc agtgcgtttg aagccgggca gtttattatg gattatctca aaacccgcgc
    13621 accgttctgg aagcgcgaag ccacgccgga aggcgaccgc tgggttgaag ctcgggagag
    13681 cgatcagcag gcggcaaaac gctggtagtt ttttgttagc cggataaggc accgctgcgt
    13741 ccggcaattt caccgctcag tacatgcgtt tacgctgcgt cgatgcacag cctcctcatt
    13801 tgcagtatcc ttaagatatt ccttatatct tcaggagatc gtcatggaca gattcccacg
    13861 ttctgattca atcgtacaat cccgggctgg cttgcaaact tatatggcgc aagtctatgg
    13921 ctggatgacc gttggcttgt tgctgaccgc atttgttgcc tggtatgcgg ctaattccgc
    13981 ggccgtgatg gagctgttgt tcactaaccg tgtcttttta atcggtctga tcatcgcgca
    14041 attagcattg gttattgtgt tatcagcgat gattcaaaag ctgagcgcag gtgtaacgac
    14101 gatgctcttt atgctttatt cggcgctgac gggtcttacg ctttccagta tattcattgt
    14161 ctataccgct gcttctatcg ccagtacttt cgtcgttact gccgggatgt tcggcgcaat
    14221 gagcctgtac ggttacacca cgaagcgcga tttaagtggc tttggcaata tgctgtttat
    14281 ggcgttaatc ggcattgtgc tggcatcgct ggtcaacttc tggctgaaaa gcgaagcgtt
    14341 gatgtgggca gtcacctaca tcggcgtgat tgtctttgtc ggattgacgg cgtatgacac
    14401 ccagaaactg aaaaatatgg gtgagcagat agatacccgc gacacgtcga acctgcgcaa
    14461 atattccatt cttggcgcgt taaccttgta tctggacttc atcaacctgt tcctgatgtt
    14521 gctgcggatc ttcggcaacc gccgttaatt ctttttcacc atcactatcc cggccatgtg
    14581 tcgggatttt tttcttacta aaaacgacac ttaacaagta ccaggtataa aatacgcctt
    14641 atggccgagc ggaagtgttt tccgtatcca taaatacaaa agtattttgt atgcgtggtt
    14701 ttaaatttat tgatggtgaa tattaatatt ttttctaagg acaactaaaa gaatggaatc
    14761 atactcgaaa aacagcaata aattagattt tcagcacgag gccaggatat taaacggtat
    14821 atggctcatt accgctttag gtttggtggc aaccgcagga ctggcctggg gagctaagta
    14881 tctcgaaatt acggcaacca aatatgattc actaccaatg tatgtcgcca tagggttatt
    14941 attactttgt atgtatggct taagtaagga tatcaacaag ataaatgccg ccatcgcagg
    15001 cgtaatatat ctgtttttac tctctttggt ggcgattgtc gttgcaagtt tagttcctgt
    15061 atatgccatt atcatcgtgt ttagcactgc gggcgcgatg tttttaatca gtatgctggc
    15121 cggtttatta tttaatgttg atcctggttc tcaccgtttt atcattatga tgacgttgac
    15181 agggttggcc ctggtaatca tcgtgaatgc ggcattaatg agtgaacggc ccatttgggt
    15241 aataagttgc ttaatgattg tgttatggtc aggcattatc tcgcatggac gaaataagct
    15301 ccttgaattg gcggggaaat gccatagtga agagttgtgg agtccggttc gttgcgcttt
    15361 tacaggtgca ttaacactct attactattt tatcggcttc tttgggatac ttgccgcgat
    15421 agctataacg cttgtctggc aaaggcatac gcgttttttt cattagccgt aaatgcacat
    15481 actaaagaac ttaactaaac ttcacatcgc cgcttcattt ttcgcccgta gcttcttcgc
    15541 ctggctttcc agcaacaggt aacagatcag cgccagcagc agtgggataa agtaatacag
    15601 cacacggtaa gcgagtaggg cggcgataat tgtacctttg gaggtatgct ccccagccag
    15661 tagtgcgata aacaccgctt ccagcacacc gatccccgcc ggaatatgca cgatgacgcc
    15721 agcaatacta ctaaccagta acacgcccag tacaaagaaa tagttcacgc tttgaccaag
    15781 taacagccag ataatcgccc ccattaccat ccagttaacg ctggaaatca gcatctgagc
    15841 gagggcgaat ttccatgaag gcagcaccag cttttgccct ttgatggtca tatggcggtg
    15901 cttcgcgaaa gcgcaaaacc acaaataaac cgcgataatc atcagtaagc caatgccgag
    15961 aatacgcagc gtagtttgat cgacatacca gtgatccggc aactccacca cgcctgcggt
    16021 aaagataatc cctgccagta aaatgtagcc cagccagttg gtggtaatac tgagcgagaa
    16081 aatccgcgta atagtgctac cgggcaaccc cagacgagag tacaaacgat agcgcatacc
    16141 aatgccgccg acccaggtac tgagcgtcag gttgaaggcg tagcagataa acgacaccag
    16201 catcacctgg cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca ggtcatagca
    16261 gccgtaaatc agatagctga cgaccaccag cccgaccgca ctaagcagcg caacgcgatt
    16321 gtagtcgcgg atgaccttcc agacctcttc ccagtccact tttttggcgt agaccaccag
    16381 caacacaatc accgcgataa aaaacagcca ggtgaggatc ttctttgcta agcgccagcg
    16441 cgggtgtgat ttactcatca gggttttacc cccgtgtttt cagtttctac ccgatcctgc
    16501 gtttccattg tcggttgtgc gggcggatca acctgcgcca gacgtggcgt gtgtgccgga
    16561 agccagccaa ccagcgccgg gaagtggcgt aaaaagtgga acgccagcac gcttttggtc
    16621 aggttccacc aggtgcgttt gggcagcatg gtttcatcca cctgctgaca atctgcggcg
    16681 ataatgccgt tcagattatc gcgcagcgtc tggttaaaat tacgatcgtg gatgatgaca
    16741 tttgcttcga gattcagtga caaactgagc ggatcgagat tactggaccc tactgtcgcc
    16801 cagtgatcgt ccatcaatgc tactttgccg tggagcgggc ggcggcggta ctcaaacacc
    16861 tgaacgccgc ctttaaccag atagttatac agcaagcgcg cgccgactct gacaatcggc
    16921 atatccggtt cgccctgaat gatcagtttg atccgcaccc cgcggcgtgc cgctttacgc
    16981 aaggcgtgta aaaatcgata gccggggaag aagtaggcgt tggcgataat gacttcccgc
    17041 cgcgcctgag tgagcatttt cagatagtgg cgttctatat catcgcggtg ttcttcgtta
    17101 tcgcgccaga ccagcaatac ctgcgcttct cccggctggc gattttcttc cgctttgtga
    17161 tgacgtcgcc accagcgtcg tgccgcgctc tgtccaggca ggttttccag ctcaaactgg
    17221 agaatatctt cgacaatcgg cccttcaagg cgtaccgcgt aatcctgttt agcctctgga
    17281 ccgtagctgg acatatgctc ggcggagtaa ttcagcccgc caataaaggc gatacgcgcg
    17341 tcgatcacca caattttgcg atgcatccgg cgaaacacat tggtgcgcat accaaaaagg
    17401 cgagggcggg gatcgtagta gcgaaatact acgccagctg ccgtcagttc attgacaaac
    17461 tcatcgctga gatccggcga accgtagcca tccagcaaga cttccgcttt aaccccgcgt
    17521 tgcgctgctg ccagtagtgc cgcatgcagc tgtttgccga cgtcatcctc aaaccagata
    17581 aacgtttcaa gaatgatgcg ttcttgtgcc tcgccaatcg ccttaaacac cgcgggataa
    17641 tattgctcgc cgttttccag caactcgatc ttattgcctt cacgccagct acatttcata
    17701 aatgaatctc cgcacttaaa ggggcatgat cagaaaggtg tcgccatgtc tgcagcggca
    17761 acgcggttgg cgcgctggcg ctggcatttt tgacgtagat cctgtccaga cgtagtagag
    17821 gaaattgcac cggaaacgtg cgcgccgggc gtccgtgggc gcgggtaaat atctcatcca
    17881 gtccggcctg cacttttaac ggatgattag ctttttgccg ccagtcattg aaatcacccg
    17941 ccaccaatac cggttcgccg tccggtagct cattcaccca ttcggcgagc atcgcaagct
    18001 gcgcctgacg gtgcgcctca cgcaggccca gatgtacgca catcacatga atcgcttttc
    18061 cggtcatcgg cggcacaatg cggcagtaga gcacgccgcg cttttccgca ccatcgaccg
    18121 aaacatcgcg attctcataa tgttcaatgg gataacgcga cagtacggcg ttgccgtgat
    18181 gcccttccgg gtatacggca ttgcgaccgt aggcaaaatc gctccacata gtgtcggcga
    18241 gaaactcgta gtgcgaggta tcgggccagt tttccacatg cagcggatga acttcgtgcg
    18301 cgcccatcac ttcctgcagg caaacaatat cggcgctgac ggtacgcacg gcgtcgcgaa
    18361 gttccggcaa aatgaagcgt cggttaaacg cggtaaagcc tttgtgaata ttgatggtga
    18421 gcaccttgaa cgaaaattgt tgtgtttgat cgggcataat tttcctgtct ttgcctctta
    18481 tctcattgaa atagtgtagt cggcgtcaca aaaaggtgcg gtcttacgga attttccgta
    18541 aagttcggta ctctgagtaa gtagagataa attcttcagg agagaagcca tgaagtggca
    18601 acaacgtgtt cgtgtcgcaa cgggtctaag ttgctggcag attatgttgc atttactggt
    18661 agtggcgctg ctggtggtgg gctggatgag taagactctg gttcacgtcg gcgtgggatt
    18721 atgcgcactg tattgtgtca cggtagtgat gatgctggtg tttcagcgcc accccgagca
    18781 acgctggcgt gaggtggcag acgtgctgga agagctgacc acgacctggt attttggcgc
    18841 agcgctgatt gtgctgtggc tgttgtcccg cgttctggaa aacaactttt tgctggcaat
    18901 tgcagggctg gcaatccttg ccggcccggc ggtagtgtct ttgctggcga aagataagaa
    18961 gttacatcac cttacgtcta aacatcgcgt acgccgctga ccctgtcgtg gccgttatca
    19021 ccagtagcgg ccacaaacta ttccacacaa tatccagact cgcatccttc aaataaatct
    19081 gcttggtaat gtccgtaaag tggcgaatag ggttaatcca cgtcaggttt tgcagccata
    19141 ccggcatgtt ttccaccgga gaaacgtaac cggaaaggag aatggcgggc atcataaaga
    19201 caaacacgcc gataaacgcc tgctgttgtg ttgaacagag tgatgaaatc aacagaccga
    19261 atcccaccag cgataaacca taaatcacca tcgtaaagta gaacagcgcc agcgatccgg
    19321 cgaaggggat ttgatacgcc cagataccaa tcgccagcac aatggtggcc tggaaggtgg
    19381 cgacaattaa cgccggtacg gctttgccga tgaagatctg ccaggtggtg agcggcgaaa
    19441 ccagtagctg atcgagcgta ccttgttcac gttcgcgggc gacggaaagt gaagtgacga
    19501 tcattacgcc gatagtggtg atcatggcga tcagtgacgg caccacaaac catttgtagt
    19561 cgagattcgg gttataccag ttgcgtacca ccagctcgct gttgttaggt ttcggttttc
    19621 cttccagcag ctcctgctga taatttttga cgatctgttg caggtagttg gcggcaattt
    19681 gcgcactgtt ggagttacgc ccgtcgagga tcaactgcaa aggcgcggtc tggaaggtat
    19741 ccagtttgcg cgagaagtca gccgggaaac gcaccagtag taacgccttt tgtgtgtcga
    19801 tggttgggcg gatctcctgt gggcttttca gcagcagcac atgagtaaag gcgctggcgc
    19861 gggcaaaacg ttgggtcagc tccaccgaat gctcgccgtt atcttcatcg tagatggcga
    19921 tggtggcgtt agtcacttcc agcgtggcgg cgaacgggaa caggatcacc tgaattagca
    19981 cgggtaaaat cagaatcgcg cgggtttgcg gttcgcgcag caacgactgc aactctttgc
    20041 ggattaacgt ccataagcga tgaaacatgc tcttctccct aatccagccg acgtttggtt
    20101 ttcagccacg tcaggccgat aaacatcacc gccgaagcga tcaaaaacag cacgtttacc
    20161 accagcacca ctggaatatt cccggcgagg aacaggcttt gcagggtgct gacgaaataa
    20221 cgagcgggaa taatgtacgt caccgcgcgg atcaccgcgg gcatactgtc gatctgaaaa
    20281 ataaagccgg aaagcataat cgacggcaga aaagcggcgt tcagggcgac ctgagcggca
    20341 ttgaactggt tgcgggtaat cgtggaaatc agcagcccca tccccagggt actgagtaaa
    20401 aacaggctgg agataaaaaa cagaatcagc agcgacccgc gatacggcac gccgagaata
    20461 aacactgaca ccagcataca cagcaacatc gccagcatcc cgagaaagta ataagggatc
    20521 agcttacaca gcagcagttc cgtgcgggta atctccgtag agagcagagc ctccatggtg
    20581 ccgcgttccc attctcgcgc caccaccagc gaggtgagaa tcgcgccgat gaccgtcatg
    20641 ataatggtca ccgcaccggg gataatgaag tgctggctaa tcgccgccgg gttaaaccag
    20701 tagcgggttt gtacatcaat aagcggttca aaagtctgcc cgttgtcctc cgctcgctgc
    20761 atttgccaga tctgccagat cccttcgaca tacccctgta caaagttagc ggtattcggc
    20821 tcactgccgt cggtgatcac ctgaatcggt gcggtggcgt tggcgcgctc catctgttcc
    20881 gcaaaatcca ccggaataac caccagaccg cgaattttcc ccgcctgcat tttggcgatc
    20941 agttcctgac ggttatcgct gatggtggcg tcgatgtagg gcgaaccggt catggtgtgg
    21001 gtgaaatcca gcgccgcttc gctacgctgt tccagtaaaa tcccgacccg cagcttgctg
    21061 gagtcgaggt taatgccgta accaaaaata aacagcagta gcagcgggat cactaccgca
    21121 atcagccagc tactcggatc gcgaacgatc tgccgcgtct ctttaacgca cagcgcccgt
    21181 acgcgacgcc aggacaggat cgggttactc attgctatgc tccttatccc agtcgtggat
    21241 caactgaata aaggcttgct ccatggtggg atcgggttgc tcatcgttag ccgactgtgc
    21301 tttcaaatcg tccggcgtgc cgctggcgat taatttcccg cggtacacca ggccgatgcg
    21361 gtcgcaatat tccgcttcat ccataaagtg ggtggtgacc atcaccgtga cgcctttctc
    21421 taccatgctg ttgatgtgca gccaaaattc acggcgggtg agggggtcaa cgccggaagt
    21481 cggttcgtcg agaaacagaa tgtccggttc atgcatcagc gaacaggcca gcgccagccg
    21541 ctgtttaaaa cctaatggca gttcatcggt ggcgtgggag gcgatacttt tcaggccgaa
    21601 cgcctcgctc atgcgggaga ttttttcgtt ctgcgcccga ccgcgtaagc catacacacc
    21661 agagaaaaag cgtaaattct gttcgaccgt caggttaccg tagagcgaaa atttttgcgc
    21721 catatagccg agatgctggc gcgctttacc ggaactctct ttcagatcca tccccagcac
    21781 cagcgcctgg ccggaagtcg gcaccagcaa accgcacatc atcttaaagg tggtcgattt
    21841 acccgcgccg tttggcccca gcaaaccaaa aatctcccca cgtttaacgg caaagttgac
    21901 gtgatcggtg gcggcaaaat ccccaaattt cttggtcagt tctttcgctt cgatcaccgt
    21961 ctcgccgggt gtgccttcta ccgtatgtaa tattgcgccc agcggcgatt ccgaggttcc
    22021 ggcaccgccc agcaaatcaa taaacgcatc ttcaaaacgc ggcgtagttt cgttgatgtt
    22081 gatttccggc atcccgtcgg catggcgaat atcgtctggt gtggcctctt ttttgaggat
    22141 cagacgtacc gatttcccct gaatcatgcc gtcgctgacc tgcggcagtt tcaaggcgcg
    22201 ttgcaacagt ttgcggttgc cctcgtgtgg actggtcatc agaaagctgc gtccggccat
    22261 ggtttgtgtc agggcttttg gttctccctg atacagcaac tcgccttcgt tcatcagtaa
    22321 cacgtcacgg cactgctcgg cttcgtcgag atacgaggta ctccagagga ttaacatccc
    22381 ttcgcccgcc agctcatgca ccatctgcca cagttcgcgc cgtgagatag ggtcaacgcc
    22441 gacgccgggt tcatcgagca gcaacacttt cggttcgccc accagggtac aggccaggcc
    22501 gagtttttgt ttcatcccac cggagagctt gcccgccagg cgtccggtaa acggcccaag
    22561 agacgtaaac tccagcaggc gagcaaaagt ttgcttacgt gcctcgccgg tgacgctgcg
    22621 caaatccgcg tacagattga ggttctccat caccgtgaga tcttcataca gaccaaattt
    22681 ctgcggcata taaccgagca cggcgtgcag cgcgccgtcg tttttgatcg gaccaaagcc
    22741 aatcaccgtg gcactgccgc tgtcgggttt cagtaatccc gccaacatcc gcatcagcgt
    22801 ggttttacct gcaccgtccg gccccaccaa ccccgtcaca taaccggcgt gaatggtaca
    22861 atcgagcggc gcgacggcgg gcttgtccat gcccggaaag cgtttttcca ggccgttcag
    22921 cgtgataacg gcatcattca tgtcctgcct cgtcaccgaa ttgtaccgtc actggcattc
    22981 cctggcgtaa cgcatcatcg gcgtcggtca ccacaatacg caggcgatag acgaggtcgg
    23041 tacgcagatc cggcgtttcg acggttttcg gggtaaattc agcagtcggc gaaacgaaac
    23101 caatctgccc gtgatacggc ttgtccgggc gaccatcggt ataaagcagc actttgcgcc
    23161 ccggctgggc ctggtcaaga ttacgttcat caacataagc gcgcacccac accggacgcg
    23221 ttagtgaaac ggtaaacacc gtgccacctt cattgaggac cgtgcctggc tccaccgcgc
    23281 gcgttaacag cgtgccatca gacggggcta tcaacgttga gtcctgtaaa ttcaactccg
    23341 cctgcgccag ttgcgcctgc gcctgttcga ggctggcttt cgcctgagcg atgtcctgtt
    23401 cacggttacc ggaacggtac tgacgcaatt tatcctgtgc tgatttcagc gttgcctgcg
    23461 cctggtcgcg cgaggagcgg gcattttcca ggtcatttgc cgaaatagtg cggcttttcc
    23521 acaacccttg ctggcggtta tagaagttct gcgcatagtc ataggcggct tgcgcctgtt
    23581 tcaccgctgc ggcggcctga gcgatttctt catcgcgata cccggcaagc atcaggtcat
    23641 actgcgcctg tgccaccgaa acacccgctt tcgcctgcat cagggcaatc tcatacggct
    23701 tgtgatccag ttcgcccagc acctggcccg ctttgatagc atcaccttcg tccaccgcca
    23761 gcgattcaac gcgcccccca acacggaaac taagatttac cgtacgaata tccacgttgc
    23821 catacagcgt caggccgtta tcctggcggc tttgatacca ccagtagcct ccggcaacca
    23881 cggcggcaag taccactacc gccaatccga tcacgacagg ttttttcatc actacaaact
    23941 cctttgcgat aatccttgca aaatcaggtc gatatgacag gtcaccgtct ggttgatcag
    24001 ttcggtcttt tcttcatcga acgcggtcca gccggtacgt aacagaattg tttctttgcc
    24061 aagacggaac gccagaatct caccaatcaa cgcatgggta tgaaggatca tgcgggtgtc
    24121 attggcgtcg cagccggtcc aggcggcaat caggcgtgtc aggtggctgt gtagcggact
    24181 aatcacctgt tcgtgcacca ggtggtaggc tgccgtggga gagagctgct cacgggagat
    24241 aaacttgctg aggttgacgg tgtcatcctg ggtgagcagc ttaatcatgt tcctgcaggc
    24301 gcgaaggatc agttcacgga tggcagcccg atcaggctgt ggttgcgcga acaagcgttc
    24361 ggcttcctcg gcatgcggac ggaactgctc gccaataaaa tcggcaatcc actgggcgca
    24421 ggcgaggtac aaatcttctt tcgaaccgaa gtagtaggtg atggcagcga tattctgccc
    24481 ggcctgggcg gctatctcgc gagtggtggc gttcattcca tattcaccaa actgcgccag
    24541 tgcggcagca atcagctgtt ttttcgcctg ttcacccttg attgtcatgg caggattatt
    24601 catcgcacag tccattctta atcaaatgat tgattaagat tatgactcca taggggagtt
    24661 gtccagtatg gctaagaatt ttagcaacgc cagtcacagg gataatttat gcgctgcgtc
    24721 acaaaaactg ctacactccg ctccctcatg acattgtggt ttttgtcatt ttccttttca
    24781 gtatctccct gaaaactaca ccggtaacgg tcggggcggt tcggagtagt tatgtctttc
    24841 gattctttgg gtttaagccc tgatatcctg cgcgccgttg ccgagcaggg ttaccgtgaa
    24901 cccaccccta ttcagcagca ggcgatccct gcggtgctgg aaggccgcga cctgatggct
    24961 agcgcccaga ccggcaccgg caaaacagcg ggctttacgc tgccgctgtt gcaacacctg
    25021 atcactcgcc agccgcacgc caaagggcgt cgtccggtac gtgcgctcat tcttaccccg
    25081 acccgtgaac tggcggcgca gattggcgaa aacgtccgtg attacagcaa atacctgaac
    25141 attcgttcgc tggtggtgtt tggtggtgtg agtattaacc cgcagatgat gaaactgcgt
    25201 ggcggcgttg atgtgctggt ggcaaccccg ggacgtttgc tggacctgga acatcagaat
    25261 gcagtgaagc tggatcaggt tgaaatcctc gtcctcgatg aagctgaccg catgctcgac
    25321 atgggcttta tccacgatat ccgtcgcgtg ttaacaaaac tacctgcgaa gcgccagaac
    25381 ctgttattct ccgcgacctt ctctgacgat attaaagccc tggcggaaaa actgttgcac
    25441 aacccgctgg aaatcgaagt ggcacgccgc aataccgcgt ctgatcaggt gacccagcac
    25501 gttcactttg tcgataagaa acgcaaacgc gaattgctgt cgcacatgat tgggaaaggg
    25561 aactggcagc aggtgctggt gtttacccgt accaaacacg gcgctaacca tctggctgaa
    25621 cagctcaata aagatggcat ccgtagtgcg gcgatccacg gcaataaatc gcaaggtgcg
    25681 cgtactcgtg cgctggctga ttttaaatcg ggcgatattc gtgtactggt ggcaactgac
    25741 atcgctgcgc gcggcctgga tattgaagag ctgccgcacg tcgtcaacta tgaactgcca
    25801 aacgtacctg aagattatgt ccaccgtatc gggcgtaccg gtcgtgcggc tgctaccggt
    25861 gaagcgttgt cgctggtgtg tgttgatgaa cacaaactgc tgcgtgatat cgaaaaactg
    25921 ctgaaaaaag agatcccgcg cattgcgatt ccgggctatg agccggaccc gtcaatcaaa
    25981 gccgaaccga tccagaacgg tcgccagcaa cgtggcggcg gcggtcgtgg gcaaggtggt
    26041 ggtcgcggtc aacagcaacc acgccgtggg gaaggtggcg caaaatctgc aagcgcgaaa
    26101 cctgcagaaa aaccgtctcg ccgcctcggc gatgccaaac cggcaggcga acaacaacgt
    26161 cgccgccgtc cgcgtaaacc tgccgctgcg cagtaatctt ttatgccggg ctatgcccgg
    26221 catcaggctg atgaacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    26281 aactctgcaa tatattgaat ttgcatgctt ttgtaggccg gataaggcgt ttacgccgca
    26341 tccggcattt cacaacaagc acttgtcagc aatttgagaa cacgggaaaa aatttatctg
    26401 ttttaccgcc cataagccac cgagacgtta cttctctata gccaattgtt cgcgcaactc
    26461 cattaaaagg tagcccagtc gattcttgcc cttaccatga ccaccgtctc cccagtaagc
    26521 atcgttttcc gtatgctcaa ccagttttgc gggcgcggtt gccagcaaga gcgcacgcag
    26581 ttctgcatgc tgttcgaatt tagcgcgaag tgcttttcgc atcacctgtt ctttgaccga
    26641 ctcccagttt ttacgcagag gcttagaacg atcgcgcccc atgcgtgcgg cgaccatagg
    26701 ggaagaaacc cgacggatct cttcgcggta tttttcatca aggaattttt gtgcctgaaa
    26761 atagtgttct gaggtgggcc aggttttccc gtcaaccttg atgggccagg cggcaaagtt
    26821 agaaaaatca ccgtagtcat cgctggtgct gtagaaattt atgatggtgt cttgcatgac
    26881 gtgttggatc ctttgtgctc gaacgggcat taaaccgcat tatgttggtg gttattgcga
    26941 gccgctttcc agaaacagaa aaaccattac ccctgaaaac cgaaaaatgc cacaatattg
    27001 gctgtttata cagtatttca ggttttctca tggcattaac cgccgcgctt aaagcgcaaa
    27061 ttgccgcctg gtataaggcg cttcaggaac agatccccga ctttattccc cgtgcgccgc
    27121 agcggcagat gattgcggac gtcgccaaaa cgctggccgg agaagaaggg cgacatctgg
    27181 cgattgaagc ccccaccggc gttgggaaaa cgctctctta tttgattccc ggcatcgcca
    27241 ttgcccgcga agagcaaaaa acgctggtgg tgagtaccgc caacgtggca ttgcaggatc
    27301 agatttacag caaagattta ccgctgctga aaaagatcat tcccgatctt aaattcactg
    27361 ccgcttttgg gcgtgggcgc tacgtttgtc cgcgtaatct gacggcgctc gccagtactg
    27421 aacccacgca acaggatctg ctggcgtttc ttgacgacga actgacgccg aacaatcagg
    27481 aagagcaaaa acgttgtgcg aagctgaagg gcgatctcga tacttataaa tgggatggtc
    27541 tgcgcgatca tactgatatc gctatagatg acgatctctg gcgtcgttta agtaccgaca
    27601 aagccagctg cctcaaccgc aattgttact actatcgtga atgcccgttt tttgtcgctc
    27661 gtcgggagat tcaggaagcg gaagtggtgg tggcaaacca tgcgctggtg atggcggcga
    27721 tggaaagcga agcggtattg cctgacccga aaaatttact gctggtgctg gacgaaggcc
    27781 atcacctgcc ggatgtggcg cgggatgcgc ttgagatgag cgccgaaatc accgcgccgt
    27841 ggtatcggct acagctggac ttgttcacga aactggtcgc tacctgcatg gagcagtttc
    27901 gcccgaagac catcccaccg ctggcgatcc ctgaacgttt gaatgcgcat tgtgaagagt
    27961 tgtatgagct tatcgcctcg ttaaacaaca ttctcaatct ctacatgcct gccgggcagg
    28021 aggcagagca ccgttttgcg atgggcgaac tgccagatga agtgctggag atctgccagc
    28081 ggctggcaaa actcaccgag atgctgcgtg gcctggcgga gttatttctt aacgatttaa
    28141 gtgagaaaac cggcagccat gacattgtac gtctgcatcg gttgattttg cagatgaacc
    28201 gcgcgttggg gatgttcgag gcgcaaagca aactctggcg gctggcttcg ctggcgcaat
    28261 cttccggtgc accggtgacc aaatgggcga cgcgggaaga gcgcgaaggg cagctacacc
    28321 tctggtttca ctgcgtggga atacgtgtca gcgatcagct ggaaaggctg ctgtggcgca
    28381 gtattccgca cattattgtc acctccgcaa ccttgcgttc gctgaacagt ttttcgcgtt
    28441 tgcaggagat gagtggtctg aaagagaaag cgggcgaccg ttttgtggcg ctggattccc
    28501 cctttaacca ctgcgaacag ggcaaaattg ttattccccg gatgcgcgtt gagccttcca
    28561 tcgacaacga agagcagcat attgccgaaa tggcggcctt tttccgtaag caggtggaga
    28621 gcaaaaaaca tctcggtatg ttggtactgt ttgccagcgg acgggcgatg cagcgctttc
    28681 tcgactatgt gacggattta cgtctgatgt tgctggttca gggcgatcag ccgcgttacc
    28741 gtttagttga actgcaccgc aaacgcgtcg ccaacggtga gcgcagcgtg ctggtgggct
    28801 tacagtcatt tgccgaaggg cttgatttga aaggtgatct gctcagccag gtgcatatcc
    28861 acaaaatcgc ttttccgccc atcgacagcc cggtggtgat caccgaaggg gaatggctga
    28921 aaagcctcaa ccgctatccg tttgaggtgc aaagcctgcc gagcgcctcg tttaacctga
    28981 ttcagcaggt tgggcgactg attcgaagcc acggttgctg gggcgaagtg gttatctacg
    29041 acaaacgctt gctgaccaaa aattatggca agcgactact ggatgcatta ccggtatttc
    29101 cgatagagca accggaagtc cctgaaggta tagttaaaaa gaaagaaaaa acgaaatccc
    29161 cacgccgtcg gcggcgttaa tgatgtgagt caggtaagga gtcgtaaatg gactatcgca
    29221 aaatcattaa agagatcggg cgcgggaaaa accacgcgcg cgatttagac cgggatactg
    29281 cgcgcggtct gtatgctcat atgctcaatg gtgaagtccc tgaccttgag ttgggcggcg
    29341 tattgattgc gctgcgtatc aaaggagaag gggaagcaga gatgctcggc ttttacgaag
    29401 ccatgcaaaa tcacaccatc aagctgacgc cgccagcagg caagccgatg ccgattgtca
    29461 tccccagtta caacggcgcg cgtaaacagg ccaacctgac gccgttgttg gcgattctcc
    29521 tgcacaaact cggttttcct gtggtggttc acggggttag cgaagatcca acccgcgtgc
    29581 tgactgaaac catttttgaa ttgatgggta ttacgccaac gttgcacggc gggcaggcgc
    29641 aggcgaagct cgacgaacat caaccggtgt ttatgccagt cggcgcgttt tgcccgccgc
    29701 tggaaaaaca actggcgatg cgctggcgga tgggcgtgcg taacagtgca cataccctgg
    29761 cgaaactggc gacgccattt gccgaaggtg aggcgctgcg tctttccagc gtttcgcatc
    29821 cggaatacat tggacgcgtc gcgaagttct ttagcgatat cggcgggcgg gcgctactaa
    29881 tgcatggtac tgaaggtgaa gtgtatgcta atccgcagcg ctgcccgcag atcaatctca
    29941 ttgaccgtga agggatgcgg gtgctgtatg aaaaacagga cactgctggt agcgagttac
    30001 tgccacaagc aaaagatccg gaaaccacgg cgcagtggat tgagcgttgc cttgctggca
    30061 gcgaaccgat tcccgaatcg ctgaaaatcc agatggcttg ctgcctggtg gctacgggtg
    30121 aagcggcaac tatcagcgac ggcctggcgc gcgttaatca ggcattttaa ttcttttctc
    30181 agccggatga gccatgtcat ccggctttta ccccaccgca atatgaaatt cctgcatctt
    30241 tattgacctt cccacgcccg gcgtgcagca taaaaataca acaaacacat aacataaaca
    30301 ggagttaacc atggaaagtg gtcatcgctt tgatgctcag acgctgcaca gttttattca
    30361 ggctgtattt cgtcagatgg gtagcgagga acaagaagcg aaattagttg ccgatcattt
    30421 aatcgcggca aacctggcag ggcatgattc acatggtatt ggcatgatcc caagctatgt
    30481 acgctcctgg agtcaggggc acctgcaaat taaccatcat gccaaaaccg ttaaagaggc
    30541 gggggcggcg gtcacgctcg atggcgatcg cgcatttggt caggtcgcgg cacatgaagc
    30601 gatggcgctg gggattgaga aagcgcatca gcacggtatt gccgccgtgg cgctacataa
    30661 ctcgcatcat atcggccgta tcggttactg ggcggagcag tgtgcagcgg cggggtttgt
    30721 ctctatccac tttgttagcg tggtcggtat tccaatggtc gcgccgttcc acggtcgcga
    30781 cagccgcttt ggcaccaatc cgttctgtgt ggttttccct cgtaaagata atttcccgct
    30841 gttgcttgat tacgccacca gcgccattgc atttggcaaa acccgcgtcg cctggcataa
    30901 aggcgtcccc gtgccgccag gttgcctgat tgacgttaac ggcgtgccga cgaccaatcc
    30961 ggcggtaatg caggagtcgc cgttgggttc gctgttgacc tttgccgaac ataaaggcta
    31021 cgcccttgca gcgatgtgtg aaattcttgg cggggcgctt tccggcggta aaacaacgca
    31081 tcaggaaacg ttacaaacca gtcccgatgc cattcttaac tgcatgacca ctatcatcat
    31141 caacccggaa ctcttcggcg cgccggattg taacgcgcag accgaagcct ttgccgagtg
    31201 ggtgaaagcc tcgccgcatg atgatgataa gccgattttg ctaccgggcg agtgggaagt
    31261 gaacacgcgt cgcgaacggc agaagcaggg gattccactg gatgcgggaa gctggcaggc
    31321 catttgtgat gcagcgcggc agattggtat gccggaagag acgttgcagg ctttctgtca
    31381 gcagttagcc agctaaaaaa aagcccgtcc agtggcggac gggcaaacaa gggtaacata
    31441 ggatcaatga gggttagagc atatgcgtct gtcggcaaac agacagggaa atacttgtgc
    31501 tggacgtagc gtaaacgcct gatccgtcca acggttcgga ttttgtaggc ctgataagac
    31561 gcggtagcgt cgcatcaggc atctgttgcc ggatgcggcg taaacgcctt atccgtccta
    31621 cgaattactt ataaattacc gcagtaccgc ttaatttatt gttgttggtg gcggaagtga
    31681 tgctgtatcc actggcaccg gctgccgcgg ctttctcagc cagtttcgct tccagggcat
    31741 cgagggtgga tgcgccatcg gcagaaacca cgccgatttt attcatgttc tgtgcctggg
    31801 atgccgttac cggttccgcc gcgaacacgc caaatgacag agttgaaaga gccatagcag
    31861 caacaacagt attgatagtt ttcataatta atctctcgca ggtgattttt gtataagtga
    31921 cgttgtttcg tcgatgtgat gagtatcacg tttttttacg agagataaaa tcgaagagaa
    31981 ttgaccgcct tgttcaaata aattgattga tatctaacct actgataaac catttatttg
    32041 ttgtacttat gaacttgttt tataccgcgt ggcagtcaca gcagcgttaa aggcacggaa
    32101 tgccacgcaa aatacaaatt atactcgcta tgcatctcgc cagttgtagt taacgtaact
    32161 ggctgtcttt cgaacctctg cgattatatc ctgtataagc tggtttttgt aaatctttct
    32221 cctgctgaca atgaatacat aagcgcacgc caggaatggc ttcccgacgg gcctgcggga
    32281 tgggggcacc gcactcttca cattcatcca ggctttcgcc gcgcggaatt tcaccccgag
    32341 cgcgggcaat cgcatcttca attgtactgt tgatctgttc gttgacggcg tcatcgttag
    32401 cccaaccgga tgccatatcg acctccccat atcaatactt gtacagttaa gtgtagctaa
    32461 tccagggacg aactcgggca gttcaagcat cagatctccg accattcccg cagcagatta
    32521 tgataaagat taagcagcga caggatctct tcactttcgc cgtagcggct tttcagcgac
    32581 tgaatattgt tgtccagttc aaacagcatg gcgcgctttt tatcatcgcg gatcatcgac
    32641 tggatccaca taaatgatgc cactcgtacg ccgcgggtta cgggtgtcac gcaatgcagg
    32701 ctgctggagg gatacaacac gagatcgcct gccgggagtt ttacccgatg ttgtccgaag
    32761 gtgtcattaa cgaccagttc gccgccgtcg tagctttgtg gatcgcttaa aaacagcgtg
    32821 gtagaaaggt cagtacgcat ccagccgttt tgtggatggc tgcgtactgc gccatccaca
    32881 tggaaaccat aggtttcatt gttctgatag cgattaaaca gcggcgtgga aagggtacgc
    32941 ggcaaggccg cggcaaagaa taaagcatgt tggttaaccg cgttcagcac ctcattttgc
    33001 agggcggcgt ataacgtgct gcgggtgtcg acctgttgat tgttcttaac ttgcgcacct
    33061 tgtgcgccgg tggtgacgcg tccatccacc cattcggctt gttccagttg ttcgcgaaaa
    33121 cgagcgacgt cctgtggcga taacacgccg ggaatgtggt acatcataca atttctccaa
    33181 aaagtggggc ctgcgcccca catctgattc agaaatgcat attggctgtg agcaagaagg
    33241 ttcttggctc gcccgggtga taacggtagc cgctcttgtt gattgaggcg acgtaatcgg
    33301 tatcaaacag gttgtaaacg tttagctgga agtcgagatt gcgattaact cgatacccca
    33361 gtttggcatc ggcgacccag taaccttcgg taaacgctgg cgttcccacc gcgccgtctg
    33421 aacctttatg catactgccg atatagcgtg cgcccgcgcc aacagagata tcgtcggttg
    33481 cctgatattg gctccataag gtgaaggcgt gctccggggt atacggcagc gatgaggaac
    33541 catcctgggc aacatctttg ccgtttttga tggttgcttt ttgctgggta tagccgccaa
    33601 tcacctgcca cgcgggagtg atattcccgg ccacggatat ctcatagcct tcgacgcgtt
    33661 tcttaccgta ttgcgagtaa gtcccgtcat cattttgctc aacttcattt tcgatatcag
    33721 tacggaacag cgcggcggtg agcaacaggc gtttatccag aacctgccat ttggtgccaa
    33781 tctcgctggt gttggctttt tgcggtttaa aatcggtgcg gttggcactg ttaccgctgc
    33841 cagactgcgc aagggcgaag ttgttgccgc ctggaggctg ctgggaaacg gcatagttaa
    33901 tatagacatt gccgttttcc gtcaggtgat acagcgcccc ggctttccag ttcaccagat
    33961 tgcccgacct ggcggtgtcg acggtggtga ccggagaacc ttttgccaca ccagttgggc
    34021 aggtgatggc accgcgtccg ctgccgccgc aggcggtggc actgtcatat tcagtatgat
    34081 aattatccag acggatcccg ccgttcagct caaaatcacg ggtgatttgc agcgtatcaa
    34141 aggcgtaaat tgcgaaggta tccgtctgac cattggcgtt tgcgccgttg cgcgtcaggc
    34201 cgccgggatg aatgctgctg tcaggatgat aaatatttac cgcgggtaac gtcaccggat
    34261 taacgccgta gttagtctgc gtttcacggg taaattccac gccggtactg acatcatgac
    34321 cgatagaacc ggtatagaac gtcgaggtca ggttggtctg gttggtgaga attttattac
    34381 tcacatcttt ggtattcgcc gtgcgtgacc aggtccagct attcacatcg ctggtgggct
    34441 gagtaatatt cgacgccccg cccataatcg ccgtcatcag gtaatcctgc tttacgcgcg
    34501 accaacgggt agtattgcga atggtggtgt tatcgttgat gtcgtgctca aaacgcatgg
    34561 tggcggtgtc ggtggtcgaa tcgtcgtaat cggaatccgt gccgtaaaag ttatgagtat
    34621 caacttttcc ggaatgattc agggccgccg ttcccgcaga tggggcagaa tagcccggca
    34681 aaccgatggt cggaatgccg ccgtctggcg tgttgtgctg ggtgacatgc agataattaa
    34741 gatacaaacg attcgctgta ccaaggccaa aagcgacaga aggggcgacg ccgtaacgct
    34801 catttttgac tttgtcgcgt ccggcatcgt gcgttttttc gcccattaca ttcaggcgca
    34861 ccgcggtggt atcaccaatg acctgattga cgtccagcgt gccgcggcgg aaccaggcgc
    34921 tgccaatact ggcggaggcg tcaatgccgg aatcattgcg cggctgcttg ctgatcatat
    34981 tgatcgagcc tgtcggtgcg ctgcgcccgt agtcggtgcc ggacggccct ttaatcactt
    35041 cgacctgctc ggtattgaag gtgtcgcgcg agacgctgcc gatatcgcga atgccatcaa
    35101 tataaatact gttagaggta tcggcaccac gcatataaat ggcgtcgcca gtggtggagt
    35161 taccgttctc acccgcaaaa aacgcaccca cgccggggac gtttttgagc gcgtcggtaa
    35221 ggttggttgc gccctgatct ttaatcactt gttcagaaat taccgtcatc gtgcgggtag
    35281 tatccgctac cggacgcgag aatttcggat cggcagattg ttgtggcgca taaagcgaag
    35341 gcgttgatgc ttcgacaacc agcgtgtcat ccgcgttagt ttgcccttcg gcggcgagtg
    35401 cctgagccac aggcgtgatg ccaatacaaa gaccggcaaa gaacgtgagc gaatgaaatt
    35461 gtctggcagg gaaattgcga ttgttttcca ttttgcaggt gactttttct tatatgttga
    35521 aattttcact gccacgagaa agcctctctg cggatgcgat gccactgtta atcccgtgat
    35581 agtgctcgtg aaagtgataa taattttgat aagcattatc acctggagag attttctatc
    35641 acccattgcg cgaaaaagaa atacatttat ttacattgag ttcatatttg atgttttttg
    35701 attacaaaac agacgaaaac tgcatccaga ggcgaatgca gttacgttgg tggggttatt
    35761 tgtagatggt tgcagtaccg aacatctggt catttccgcc tgcggaatta atgacataac
    35821 ctttcgcgcc ttgttcgcgc gctttttctg ccagtttatc ctccagatcg cttaggtttg
    35881 atgcgccagt tgccgaaacg gtaccggcgg gccgtagctg accgctgtcc aggttactca
    35941 tcggttgcgc ggcataagcc gtgagagaga ttccgctcag gacggtggca atcagtagtg
    36001 tgaggcactt tttcataatg acatcctcat aaatacaacg ggtagtgcct gataagtgta
    36061 ggtcgcctgc gcgatgcaaa tgacgcaaaa aattgacaga gatgtgctta ttttttgaac
    36121 gatatatttt tacaaaataa gacaaatcaa tgacatgcat aatgctgatt tttatgatga
    36181 attgccataa accatagcca tgttaaggtt ttggggaatg ataaacgcag ccagatattt
    36241 accctgtcgc gttatgcggg tatcttacgc cgctgcttaa aggagaatgc tatgtccgcc
    36301 cagaaaccgg ggttgcatcc gcgcaaccgt catcacagcc gctacgatct cgccacgctt
    36361 tgtcaggtca atcctgaact caggcaattc ctcacgctta cacccgccgg ggagcaaagc
    36421 gtagactttg ccaatccgct ggcggtgaag gcgctcaata aggcgttgct ggcccatttt
    36481 tacgccgtag cgaactggga tatccccgac ggttttctct gcccaccagt accgggccgg
    36541 gcggattata ttcatcacct tgccgattta ctggcagaag caagcggaac aattccagcg
    36601 aatgccagca ttctggatat cggcgttggt gcgaactgta tttatccgct gattggcgta
    36661 catgaatatg gctggcgttt taccggtagt gaaaccagca gccaggcgtt aagcagtgcg
    36721 caggcgatta tcagttctaa tccggggctt aaccgcgcca ttcgtctgcg tcggcaaaaa
    36781 gagagtgggg cgatttttaa cggcatcatc cataaaaacg agcaatacga cgcgaccttg
    36841 tgtaacccgc cattccacga ttccgccgct gcggcacggg caggtagtga gcgtaaacgc
    36901 cgtaacctgg ggctgaacaa agacgatgca ctgaactttg gcggccagca acaggagttg
    36961 tggtgtgaag gcggtgaagt cacctttatc aaaaagatga ttgaagagag caaaggcttc
    37021 gcgaagcagg tgatgtggtt tacatcactg gtatctcgtg gtgaaaactt accgccgttg
    37081 tatcgtgccc tgacggacgt gggcgcggtg aaggtggtta aaaaagagat ggcccagggg
    37141 caaaagcaga gtcgctttat tgcctggacc tttatgaacg acgagcagcg ccgccgtttt
    37201 gtcaatcgcc agcgttaaag cgttggttcc cccggcggta acggttcagc cggggtcgcg
    37261 cccggagcag gcagcacctg ataagtctgc actggcgcgc gaacgcccgc caggtcgaaa
    37321 tgttttttca cctggctgtc gagggcaaag cgtaccgtcc actgtttgag tggcagcgtg
    37381 gtgaacgaaa cacgcagtgt aaacgcggta ttgcttaagc cgacaatccc ggcaaaattc
    37441 ggttcaccaa taatcagccc gcgaatttct tcgttttcca ttaattccgc taccgcatct
    37501 ttcagtgcct gattggcttt atcagcatct tcatggcgat caacatcata atttgccacc
    37561 accgaaccaa tgccgcggac aaagttagca aaggtggtta tcgaagacca cggaatgatg
    37621 tgatacgccc cggtatcctg tcgtacgccc acggagcgaa tcgacatccg ttccactgtg
    37681 ccggtcaacg gcccgatagt caccaaatct ccagtgttca tgccgttttc aaactgaata
    37741 aataccccgg tgataatatc tttcaccagc gtttgcgaac caaacgagat agccagacct
    37801 aatgccccgg cacctgccag caatggcgcg atattgacgc cgatttccga caacacaatc
    37861 atgatggtga tggtactgat aatcaccgcc agcgcgttac gaaacagcgt aagcagggta
    37921 cgcgtgcggg cgctgggtag cgggcggcca tgaatatccg aagccagccg gttttcgatc
    37981 aaactggcga gcaccgtcca gccaaccgcc gagaagaaaa gaatgagtgc gatacggatc
    38041 aggatatcta cggttttctg ccccgcgccg ttttgcagcc agttccagaa atcgaacaat
    38101 ccccatgcgc tcaacagcaa cattaccgcc acgcagactg tcagaatacg cgccgttttc
    38161 agcgccgccg acagccagcc attcaaccgt ttttgcagct ccggatagtt acgctgagta
    38221 tgtggcgaga gagtgatggt tttcgccagc cagcgggaaa acataccgga aacaaacgct
    38281 gcgataccaa taatcgccag gctgcgcacc gttgcaccca tcataaattt caggctgttg
    38341 cccggatcga acaacgaaaa gaaaaacagc acgataaaat aggcgcttgc cagccagtgc
    38401 cacaccagcg caaaggcgcg gataaacagg ctgaaaaagg ccagcgaatg ctccgcgaag
    38461 ttgagcaaat gctgggtaat ctctttttta ttacgaaaga tcaggtacaa cgcccagacg
    38521 gtcatgcaca gcataatgat gacgttcgcc agcgcaccta tctgtacatt cacctgatta
    38581 gagataatcg gcacggccac aatcaggcca taacctatca aactgcttaa ccagctcagg
    38641 cgacgactcc agtaacgggc gctctcgtcc tgaatcgtga acgggcgcag ctccgccacg
    38701 tttgggcaaa aaatcaggcg tagtacggct ttgaaaaatt caatgagggc aaaggcgttg
    38761 agaaacaaac tttgttggaa agcgatggtg cgactgcctg cattcaggtt gtcgcttaat
    38821 acctggccga caaataatgt cagggccagt aacagcaggt cgataataaa cgccccgata
    38881 atcatcgccg gaagctgcaa ccagttgctg cgctcacgat ttttttgccg cgcccactga
    38941 cccattttgc gatacagcgg cagtgcgcac aggcgaatca gccagtaaaa accaaacact
    39001 aataccgcta acattgaaaa atgggtcagc gcattgctga aggtttgtgg attaaacggc
    39061 ttatgcgggg agccggtgat attgcgataa agttgcccga agcgggcgga aagggcttca
    39121 ccataatggc ggctgacttc ggtgaccttt tgcagcacgg tttgctcttc gaccagcgtc
    39181 ggcggcacta tttttggtac cggttcagca gggggcgtag cggcaacggt gcgcaactgg
    39241 tcgatcaact ctttacgcga ggtgtcatta tccagcacat ccgccagtgc gccataggcc
    39301 gctttttttt gttcgatatc cggttccggg gccggttcag tcgttgagtc cgttgtcgtt
    39361 gtggttgtaa cgccgggtat ggataccgcg tgggcaggtg cgcccagcag gcagaagagg
    39421 atgaacagga tccaccgcat gactcctcca gtgagaaaat agagcaaaaa gataagtata
    39481 gatgctggag gaggggcgat tttaaatgag aggaatctgg tgtgcctccc tttcgggtga
    39541 aagggaggaa gggatattaa gcaggttgct gacaacgtgc gggctttttt gccggatgcg
    39601 gcgtgaacgc cttatccgtc ctacaagttc atgcaatttt aataagttgc cagatatcct
    39661 gtaggcctga taagcgtagc gcatcaggca atgtgtctta ttaagagacg tgctgcaaaa
    39721 attcctgcaa gcgctggctc ggcgggttct tgatcaacac ctgcggattg ccatcttccg
    39781 caatccggcc tttgtcgata aagatcagcc gcgaagctac tttctcggca aaaccgattt
    39841 cgtgggtcac gatcaccatc gtcatccctt cttcagccag atcctgcata accttcagca
    39901 cttcatggcg cagttccggg tcaagagcgg aagtcggttc atcaaacagc atcattttcg
    39961 gcttcaccgc cagcgcgcgg gcaatcgcca cacgctgctg ttgaccacca gaaagttcgg
    40021 aagggtagtg atgtgcacgt tctgccagac cgactttcgc cagcagctca cgtgccagtt
    40081 tttccgcctc ttctttgttc gcgccacgca cgcgtagcgg gccaaacatg acgttttcca
    40141 gcgccgtcag atgcgggaag aggtaaaact gctggaacac catacctgct tcctggcgaa
    40201 tcaggcgctc gtcaactttc ggatcgttaa ccttcaggcc atcgacaatc agatcgccgg
    40261 aggtgatttc ttccagtttg ttgatgcagc gcagcagggt cgatttaccg gaaccggacg
    40321 gcccgataat caccacgact tcgccctggg caatgttcaa atcgatattg tgcagcacct
    40381 gggttgggcc aaagtgcttg gagacgtttt taaattcaat cacaggattt tcatccttct
    40441 ttccagacga cgcagaataa agctcagcac cagggtaata atcagataga acaccgccac
    40501 ggcgctccag atctcaaggg cgcggaagtt accggcaata atttcttgcc cctgacgggt
    40561 cagttccgcc acgccgatca caataaacag cgaggtgtct ttaatgctga tgatccactg
    40621 gttacccagc ggcggcagca tacgacgcag tgccagcggt aaaatgacgt agcgaatggt
    40681 ttcccaacgt gaaagaccga gcgccagtcc tgcttcacga aaacctttgt ggatagacag
    40741 caccgcacca cgcgtgattt ccgcaatata cgcgccggag ttgatcatga tggtgaccac
    40801 cgccgcagta aatgggtcga tgcgtaagtc gttaaacgcc atcggcaggg cgaaataaat
    40861 aaacatcacc tggacgacga taggtgtgcc gcggatcact tcaataaaga ccagcgcgac
    40921 gtggttggct atccaacctc cgaaggtgcg tgcaaaacct gccagcaatc cgattaccag
    40981 accgcctgcc agaccgagga ccgaaatcca cagggtcatt ttggcacctt caatcagaag
    41041 cggaatggca ggccagatgg cactccagtc aaactgcata tgttgttcct gttaccgtgg
    41101 tgtaaataat caaattcagg gtaattaatg gccccggagc gggtttgcgc ccctcaccct
    41161 aatcctctcc ccatagggga gagggaactg ccagtgcgtt ttacaggtgt agcgttatta
    41221 tttcggttca gtaccgaacc attttttgta gatttcgttg taagttccgt tctcgcgcag
    41281 ggttttcaac gcgccgttga ctttgtcacg cagctcgtcg ctacctttcg ggaacgcaat
    41341 accgtattgc tgcgcttcca gagagtcacc taccgctttg aactgaccgt taccggcggt
    41401 tttgatgaag tacagaatgt ttggcgtatc gtgcagaacg gcgtctgcgc ggttggtgcc
    41461 cagttccata taggcgttat cgatgttcgg gaactgacgc agatctttag ttttgatgtt
    41521 tgctttcgcg taatcaacgg agccagtacc gctcttcaca gcaaccactt tcccgtcgag
    41581 atctttcacg cttttcacat cgttattgtt agctttcacc atcactaaca ggccgctttt
    41641 gtagtagccg tcagagaaat cgatcgcttt tttacgctcg tcggtgatgg taatgcccgc
    41701 cagcgccaga tcgacgtttt tggtttgcag tgccggaatg atcccactga aatccatcgg
    41761 cttcagttcg taatccagct tcagctcttt agcgatggca gcccacagat caacgtcaaa
    41821 gcccacatat ttatcgccct gtttaaattc aaacggaacg aaggcggtat ccgtcgcgac
    41881 aactaatttt ttatccgcgg catgagaaga aaccgcaaaa gccagggtca gtgcagccag
    41941 tgaaactttt aatacagact tcatagcatt tccttttttc tatccacggg acgatcccct
    42001 gcgtgacatt cacatatatg aaaaaatcgt gccagttttg caactccttg atttttcgag
    42061 atgcggcaaa aacgtgatgc acgatttata gggcaatacc ctgaagatgc accattctgg
    42121 ggcaccaatc tggtgcgcta aaattgtgca ctcaacacag tgattattta gcgtaaaacc
    42181 tgatgaaaaa acaatcttta tgtagcgatt gtgtgatgat gtggatacaa aaaatttact
    42241 taatcagctg gagatagcag atggatgcac taaataagtg cgttgaggtg ggctgcaaaa
    42301 caaaacggcc tcctgtcagg aagccgcttt tatcgggtac taaagttctg caccatcagc
    42361 gatggattta ttcgatgtta gactcgataa accacaggaa tttatccagg tcgcgagacg
    42421 cggcggtcag gatatctgcg gtgtcgtcat ctttcgcttc gccaatcgct ttgcgtacgt
    42481 cattagcgac gattgcgtaa cggtcagcca gttctttcag gtgatcctga acgttgtgga
    42541 tgtccagcgg gtaacttttc agcggggttt tgctgttgat aacttgagtg gtccccagag
    42601 ctacaccgcc cagctgcact gcacgttctg ccatggtatc cagatgatcg atcagtgcgg
    42661 tgcggaagcc atccagcatt tcatgtacgg caatgaagtt agcgccgcgc atgttccagt
    42721 gcgcttgttt ggtaatcaaa gaaagatcaa taaactggat aacctggcga ttcagcaact
    42781 ctactgttgc ttttttctcg ctgtcggaga catcgttgcg ggtataaagc agattggtcg
    42841 cttttgattt aactaattta gcggtactca taatttcata tcctcttgat gttatgtccc
    42901 agtaattaac gagactaagt atagcaccgg ctatgtgttc cgctattctg gctgttccta
    42961 tcacactaat agtggtaaca agcgtgaaaa acaaaactaa taaagattca atgagttaga
    43021 tatattgata agaacaattc ttatttatct gctcgccagg aattttcgga ataaatatgc
    43081 ttttgcataa ttcgatatat aaagacggtg tagaggaaaa gtagcgagaa attctgcatg
    43141 gttatgcata accatgcaga aatgtaaatt taattaatgt ctaattcttt tattttgctc
    43201 tctttgcgta ctgtcagcgt agaccccatt gaagcggcga taatcgcgcc gagcgccagt
    43261 agctgtatgg gtgtcagtgt ttctccgagg aaaatcatcc cggaaacggc agccagcgcc
    43321 ggttccatgc tcatcagcgt accaaatgtc cgtgttggca aacgggtgag ggcaatcatt
    43381 tccagcgaat aaggcagagc ggtcgagaga atagcgacag ccagacccaa tggaataacc
    43441 gaccagtgcc agagtgcttc accagcctga agcgctccaa ttggcacgaa aattaacgct
    43501 gcaatcaacg aaccaattgc caccgtcgca gggccatgtt ccgctcctgc gcgttgccca
    43561 cttaaaatgt aaatagccca acaagccccg gcccccagtg ccagcgcaca gccggttaaa
    43621 tcgacatggg aaacgtcttg ccccagcggt agcaggaacc acagaccaag aaccgccagc
    43681 acaacccaga cgaaatctac cgggcgacga gaagagaaca gcgccaccgc cagtggtccg
    43741 gtgaactcca gcgccaccgc aatacccagc ggtactgtct gaatagaaag ataaaaaaga
    43801 taattcatcc cacccagcga aacgccgtaa aacaacagcg gtaaccgttg ctctttggca
    43861 aagcgcagtc gccatggctt aaagaacgcg atgaggatca gcgttcctaa tgccagacgc
    43921 agcgcagtga cacccggtgc gcccaccaga ggaaaaagtg acttagctaa cgaggctcca
    43981 ccctgaatag acgccatggc aacgagcaat atgactattg gtaaccagac cggcatttta
    44041 cgtaatgaac caggcatcct ttctcccaca aatatctaga cttaagtaaa gcgtggagtg
    44101 tactggatat acccaatgct ggttgagcat ttgttgaaaa aattttcccc cgttttgact
    44161 aaaatgcgcc aggattgatg gaatcattag tctggtgatt aggaataatc tggatgaatg
    44221 acagggaaaa catgcgtaat acttacgcag ttctctgaaa aagtgattta aatttagatg
    44281 gatagcggtg tatggaaacg ttctgttaca tgaaatggcc cgttagacat cacaaatcgc
    44341 gaagagtttc ccattaattt ttgatatatt taaaacttag gacttatttg aatcacattt
    44401 gaggtggtta tgaaaaaaat tgcatgtctt tcagcactgg ccgcagttct ggctttcacc
    44461 gcaggtactt ccgtagctgc gacttctact gtaactggcg gttacgcaca gagcgacgct
    44521 cagggccaaa tgaacaaaat gggcggtttc aacctgaaat accgctatga agaagacaac
    44581 agcccgctgg gtgtgatcgg ttctttcact tacaccgaga aaagccgtac tgcaagctct
    44641 ggtgactaca acaaaaacca gtactacggc atcactgctg gtccggctta ccgcattaac
    44701 gactgggcaa gcatctacgg tgtagtgggt gtgggttatg gtaaattcca gaccactgaa
    44761 tacccgacct acaaacacga caccagcgac tacggtttct cctacggtgc gggtctgcag
    44821 ttcaacccga tggaaaacgt tgctctggac ttctcttacg agcagagccg tattcgtagc
    44881 gttgacgtag gcacctggat tgccggtgtt ggttaccgct tctaatcact ttggtgatat
    44941 aaaaaatccg cctctcgggg cggatttttg tttttaaggt ttcgggtcga aaatatcggt
    45001 tccgagatgg ttgtaatcca ccttctgtaa ctggaagttg gtgatgtata tcggcccggc
    45061 tttcttctca gatataaacg ggtatttgat gttaatttcc ttcgctttaa tccccgtcca
    45121 ctgggagaaa aagcctaaga agtcattggc tgagcggcgg gctttaatca cacgatgcgc
    45181 tttatcgtcg ctggaaatga ccataaaagg cacctggaaa ttttgctgat atttatcatc
    45241 atgggcaagg tattgcacgt ctttaccgcg ctctttaaag gccagaccgt ggtcagaaaa
    45301 gtaaaccagc gagaagctgc tgccgctgtt gcgtaactga tcgtacagct tgcgcagtaa
    45361 atcgtccgtt tgcgtcatgg tatagagata gcacgacgtt tctttcgatt gcacaaaggt
    45421 ttcgtatttt ccttgtgtcc tgtcgcaggc ctgcggatgt gagcccatca gatgtagaac
    45481 aatcagctgc ggttgcgagt gctcttgcgc cagcacttga gcggtcatat ccagtaacgc
    45541 ttcgtctttg gtgtttttat ctgcttcaaa attaccttct ttcaggaagt acacttcatc
    45601 tgctcgtttg gcgatgctgg cgatagcggt atcgtattcg ccgatttgac cctggttgga
    45661 aaaccaccag gtctggaagc ccgcgcgatt tgccagggtg acaaagttat cctgaaactg
    45721 tggtttgcca tcgacaacgc gattgagcgt taagccaagc gatttctgcg tggagccact
    45781 ggcggcaatg tagtcagcaa atatcaaacc gttaacgctg ctggcaaacg gggtattgtc
    45841 ccagtgaccg ccaaaggcac cgagggcatc gcgacgcgcg ctttcaccga tcaccaccac
    45901 ataggtctga tacttcggct taacggcagt gaccgtccag gtatctttca ttccggaaag
    45961 ttttgccatc cgttcttgct cttcaatcac ctcgttatta ttgacgatga catccttagc
    46021 aaaacgaaat accggatagc cagtatcttt cagtttaaat acgccacccc aggccaggtt
    46081 ttgcacggga gcaacaaaaa atgtcgccac actgaatacc agacacaggc tgtcgaaggt
    46141 attccagcgc gctttttcat tctctttttt tcgcctgatt gctattacgc cgagcgcaaa
    46201 aataaataag ccgaccaggt agctgtacca cggaaaaatc gtcaggattt cggtcgactc
    46261 ttccatattg gtggagtgca atgccagcaa tgtattgaaa ttaggcgcgc cgtaggcctg
    46321 agcaaaaggg aaataacaag ccgccaccag cgaactgaca ccgaccagaa ctttttgtac
    46381 gcgaggcaat gttcgccata gcaaaagcaa aatagccgta aacgcagcgg tgtagagcaa
    46441 actgaagggg taacctaagc cgaggttaat taatagcgac tgtaaaaagt agaacgcagt
    46501 ccacgggcta aatacccggc tacgggtaac aagcgattct ttgagggtta aattcatatg
    46561 ccactatcgt aacaaacgcc atgtgcttac cctggcgtca agggtcaata cctgcaagag
    46621 tgcgtgaaga gtggaaaggg gtccgcatcc gcgagccgca atatacgcag ggctgcaaga
    46681 agatagagcg agccgccgtt caggtcaact actgacgaac aaatcttttg cgaaggggat
    46741 tgcaaatccg acaaaaagtg gggtttttca gaccaaaacg cgttatgcct gcatgaaagc
    46801 aggagtgaag cggcgtaccg cgacgccgct atttatcgga aggtttatct tgctgcggtt
    46861 tgttgttgac catatcgcac aacatagaga gcagcattaa ccgtacttta aagggagaat
    46921 gactaaacac gcgcatacac ctcttgaact cattcataag acctcctgac ttgctaatcc
    46981 cgtcgatcct tgagggatga ttgcattaca tacagatata gcacaggcta tattatatag
    47041 ctattgctaa aacgttaatt ttttgtacct tcgcaactct ggtttacaat gtgcgcacga
    47101 aatgagaacg ctatgtatgc gtcaccataa tgaggaagca caatgagtcg tcgcgcaggt
    47161 acgccaacag caaaaaaagt gacgcagtta gtgaacgtgg aagagcacgt tgaagggttc
    47221 cgccaggtca gagaggcgca tcggcgcgag cttattgatg attacgttga gctgatttct
    47281 gacttgatca gggaagtggg ggaagctcgt caggtggaca tggctgctcg tctgggagtt
    47341 tcgcaaccga cggtggctaa aatgcttaag cggctggcaa ccatggggct gattgaaatg
    47401 atcccctggc gtggcgtgtt tttaacggca gaaggagaga agctggcgca ggaaagccgc
    47461 gagcgacatc agatagtcga aaatttcttg ttggtgttgg gcgtcagtcc ggaaatcgcc
    47521 cgtcgcgacg cggaaggcat ggagcaccat gttagtgaag agacgctcga cgcttttcgt
    47581 ttgtttaccc agaaacacgg tgccaaatga gtctgccttt tttacgcacg ctgcaaggcg
    47641 atcgtttttt tcagttatta attcttgttg gtatcggatt aagctttttc gtgccctttg
    47701 caccgaaatc ctggcctgct gctatcgact ggcacaccat catcacctta agcggcctga
    47761 tgctgctgac caaaggtgtg gagttaagcg gttattttga tgtgctgggg cgcaaaatgg
    47821 tgcgccgctt tgctacggag cgtcggctgg cgatgtttat ggtgctggcg gcggcgctgc
    47881 tttctacctt tctgaccaac gatgtcgcgc tgtttattgt tgttccgctg actatcacgc
    47941 taaaaagact gtgtgagatc ccggttaatc ggctgattat ttttgaggcg ctggcagtca
    48001 acgctggttc gctactgacg ccaattggca acccgcaaaa tattcttatc tggggacgtt
    48061 ctggtctttc gtttgccgga tttattgccc aaatggcacc gctggctggc gcaatgatgc
    48121 tgacgctcct gctcctgtgc tggtgttgtt tccctggaaa ggcgctgcaa taccatacgg
    48181 gggtgcaaac accggagtgg aaaccgcggc tggtgtggag ttgtctgggg ctgtatatcg
    48241 tctttctgac ggcgctggag ttcaaacaag agctgtgggg actggtgatt gtggcggcag
    48301 gctttgcgct gctggcacgt cgcgtggtgc tcagtgtgga ctggacgctg ctgctggtgt
    48361 ttatggcgat gtttatcgac gtccatttac tgacccagct tccagcgttg caaggcgtgt
    48421 tgggtaacgt gagtcatcta tctgaacccg ggttatggtt aacggcaatc ggtttatcgc
    48481 aggtgatcag taacgtgccg agtaccatat tgttgctgaa ctatgtgccg ccgtctttat
    48541 tactggtatg ggcggtaaac gtaggtggct ttgggttatt acccggatcg ctggcaaatt
    48601 tgattgcgct acgtatggcg aacgatcgcc gcatctggtg gcgtttccat ctctattcaa
    48661 taccgatgct gttgtgggcg gcgttggtgg gatatgtttt gttagttata ctcccggcca
    48721 actagggtct ggcaaataaa aaaggcggat tatgagtccg ccttttgctt taagttttga
    48781 agattaattc agacgaaccg gcatcccgga gcggttttta atcgcttcat caagaacaac
    48841 ctggtcaaca tctggctgac cggtcactgt ctgcacgctc ttcgtcaggg taattggcac
    48901 aatttcctga ccttcaaact gggcttcggt ggtagacagc gggttatgga cttcaatata
    48961 acggctgccg tctggctcgg tggtcgcttt taccggctca tcaataaact gtacgcgggt
    49021 accgaccggt actttctcga acaggaattt gatgtcttcg ttacgcagac gcacacaacc
    49081 atgacttaca cgcaggccga taccgaagtt ggcgttggtg ccatggatag catacaggcg
    49141 accgatatag agtgcataca gccccatcgg gttatccgga cctgccggaa cgacagccgg
    49201 aagcggttcg cccgcagcgc ggtactctgc gtgcattttg gcggtcggcg tccaggtcgg
    49261 gcctgctttt ttacgctcaa ctttggtggt ccagttgata ggcgtatctt tgcctaactg
    49321 accaatgccg atcggcagca cgataacggt gttggtccct ttcggatagt aataaagacg
    49381 catctcagca ctgttaatga cgatgccttc atgaacggta tccggcagga tcagctgctg
    49441 cggaatgttc agtacagtac cgcctttcgg caggaaggta tccacacccg ggttcgcttc
    49501 catcatattg gaaagcccca tctggtactc ggcggcaaaa tactccagcg gctgagtgtt
    49561 accttcagga atggtgatca cctgattctg accaaccagg cgactcccgt cggttggcag
    49621 aggataagtt accgcagagg cggtactgca aaagccgaca acagcgaagg ccgctgcgaa
    49681 taatgttttc aatttcatat tcatgttaag cgagattttg tgcctggcag gccattgggt
    49741 tgagaatatt agagtattgg aagcgcatta taagttcatt ccagctcaca gtgaaatcag
    49801 atgtgtacga aatcacattt tttgcctttg gcttgagtgt agaccttaag cgaggagcag
    49861 gatcttcttt cagacttatg gcataatgcg cggtttgtca tatctctttt caggatacgc
    49921 ctgtgttagt ttccagtaac gtcaccatgc agttcggcag taagccgttg tttgaaaaca
    49981 tttccgtcaa atttggcggc ggcaaccgtt acggcctgat tggcgcgaac ggtagtggta
    50041 aatccacctt tatgaagatc ctcggcggcg accttgagcc gacgctgggt aacgtttccc
    50101 tcgatcccaa cgagcgcatt ggtaaactgc gtcaggatca gtttgccttt gaagagttca
    50161 ctgtgctgga tacggtgatc atggggcata aagagttgtg ggaagtgaag caggagcgcg
    50221 accgcatcta tgctttgccg gaaatgagtg aagaagacgg ctataaagtg gccgatctgg
    50281 aagttaaata cggcgaaatg gacggttact ctgcggaagc tcgcgccggt gaactgttgc
    50341 ttggcgtggg aattccagtg gaacagcact acggcccgat gagtgaagtt gctcctggct
    50401 ggaagctgcg tgtgcttctg gcgcaggcgc tgtttgctga tccggatatt ctcctgctcg
    50461 acgaaccgac caacaacctc gacatcgaca ccattcgctg gctggaacag gtgctgaacg
    50521 agcgtgacag caccatgatc atcatctcgc acgaccgtca cttccttaac atggtctgta
    50581 cccacatggc ggatctggat tacggcgagc tgcgcgttta tccgggtaac tacgatgagt
    50641 acatgacggc ggcgacccag gcgcgtgaac gtctgctggc cgataacgcc aagaagaaag
    50701 cgcagattgc tgagttgcaa tctttcgtta gccgctttag cgccaacgcc tcgaaatctc
    50761 gccaggcaac ttcgcgcgcg cgccagattg ataaaatcaa actggaagag gtgaaagcct
    50821 ccagccgtca gaacccgttc atccgttttg aacaggataa gaaactgttc cgtaacgcgc
    50881 tggaagtgga aggtctgacc aaagggtttg ataacggtcc gctgtttaaa aatctcaacc
    50941 tgctgctgga agtgggtgaa aaactggcgg tactgggtac caacggcgtc ggtaaatcaa
    51001 cgctgctgaa aacgctggtg ggcgatctgc aaccggacag cggcaccgta aaatggtctg
    51061 agaacgcgcg cattggttac tatgctcagg accacgaata tgagtttgaa aatgatctga
    51121 ccgtgttcga atggatgagc cagtggaagc aggaaggcga tgacgagcag gcggtacgca
    51181 gtattctcgg tcgtttgctg ttcagccagg acgacatcaa aaagccagct aaagtgcttt
    51241 ccggtgggga aaaagggcgg atgctgtttg gtaagttaat gatgcagaag ccgaacattc
    51301 tgatcatgga cgaaccgacc aaccacctgg atatggaatc cattgagtcg ctgaacatgg
    51361 cactggaact gtatcagggc acgctgatct ttgtttcaca cgaccgtgag ttcgtaagct
    51421 ccctggcgac ccgcattctg gaaatcaccc cggaacgcgt gatcgacttt agcggtaatt
    51481 acgaagatta cctgcgtagt aaagggatcg agtaagtgat tatccgtcac cccgatactg
    51541 tcggggtgac ggcggctgat gacaaatgca aaatagcctg atgcgctacg cttatcaggc
    51601 ctacgcagta cctgcaatat attgaatctg caggactttg taggccggat aaggcgttaa
    51661 cgccgcatca ggcattaaca aagcgcattt gtcagcaatc tgagtaacgt cgaatcgatt
    51721 ttcttaatcc cacatcaatt cacttccatt aattctcaaa catccatgcc caacgctcgc
    51781 ttaccgtgaa tgttgaggtc ggcaagcgta aagcgtcctt cccagtttgt ttcatagtcc
    51841 tcgcgcggga agtcgcccgg cgatgcgcct ttttccagtg cggccttcac cttgtgcgcg
    51901 taggcaagat ttttctcgca catcggtgcc gcaggaatgt acatcacgtt gccccaacct
    51961 tgttgatttt caacgggggc aacggaatgg atgacgtcgc agtgccacca gacggagtct
    52021 ccggcttcga gttttggaat gctggttaac gcctcaatca acagtggatg ccattgctct
    52081 gataccggca atactcttcc gggcgctacg ccgcacagtt catcctccgg cacatcatca
    52141 agcagcggac gtaacagtac gtacgccata gcttcaggaa tgggcacgac gtgcagcagc
    52201 ccctgaccag gcagcatatc agagagcgct gtccagccct ggaatgtccg aaacacggaa
    52261 catttggtgg tgttgtccac cgtgtactct tcaacttccg tacgatgtgc cgcatgccag
    52321 ggatcatatt gcgccagatt gccattaaag acgttggcga aaacgcgctg atacgctgga
    52381 agcagccagc gttccagtgc cccggagtcg gtatgcgctc caagaccttt ggaggtcgtt
    52441 ccgggcggac ggcggcggat acggtcaggg tagatgacgc tcacatccgg gttaaaccat
    52501 tgctttccat cactttcaaa tgtccacaga cgattgagaa acgactgcgc attcgccatt
    52561 tcttcactct ggcgggcctg catttgcgcc tgcgaccagt agatggggta aatctcggga
    52621 cgtgaagcgc tgagcgtccc gaagaaatta tcgccggggc ctttgtagac ctcgtcaaag
    52681 cggttgcggt ccagatagtc cagcatcgac tgatcccagc ctagcgcttg ttcgcggggg
    52741 aagtggcctt ttatcaccgc acaaccgcga cgtttaatct gttcgcgctg ctctgcagta
    52801 acatgacctg ctttgatatc ggcataagac agcaccggcc agacggcatc gccctgtgct
    52861 ttgagtgcgt tgatttcagc cactcgcgtg gcaatgtcat cgcttagctg attaaagatc
    52921 tgctggacgt cgccaagctg cgcccgcagc gcgtgcttca tctgacggat agctgctttg
    52981 tgatcggcag gcaatgtgtc gctggtaaaa gtagaagcca taaccacctc gcattaactt
    53041 tcattcgaaa ctatgatgat ttgcatgtga caatataagt tataaataag ttaatgcaag
    53101 tttaatgatt tgatgtgatg tacaggatgt aaaaagaagg gaaaaccgga gcgcatggct
    53161 ccggtagaga ggtcagctgt taaaagggga tgtgttatcc agcaccgcct gaatcacatt
    53221 cagcgcgcct tcgtgattat tatcatcggt agcgtaacgg gcgatttgtt taatgttttc
    53281 cgcagcattg cccatcgcaa aggaataacg cgccattttc agcatctccg catcgttacc
    53341 gctgtcgcca atcgctacca cattttgcgg tgacagatcc cagcgtttca gtaaccgcga
    53401 aataccgttt gctttatgta gaccgggaat aatcaggtcg ataaagccaa aaccactggt
    53461 gacgggtttc ataatgccat cgagcgctac gtgcagtttg tcgatcacta acgggatttg
    53521 ttcatccgga aggttgagcg aaaacttgaa cagtacgtcg tcaatctcct gataatcttt
    53581 tacaggtttc aggcgatggt agtgttttgc catcagtgcg acaaatgctt cgggggcatt
    53641 ttcgctgaca tatgcacttt gcagaccgca ggcgacaaaa ttgagttgct tatcttttag
    53701 caactcgcca ataacaatcc gcgattcatg tcgggtcagt tcgccgtgga acagttgctt
    53761 gccatgttcg taaaccagcg cgccgttttc cgcgacaaaa gagatctcat cttttagctc
    53821 aggaaagaac gaaataagct ggtaatactg attaccgctg gcgacaacga actcaatgcc
    53881 gcgctttttc agttcctgat attgcgccat aaaacgtggt tggttgtacg ttttggcgtc
    53941 gttaagaaaa gtaccgtcca tgtctgtgac gataactttt acgctcatag gtgtgctcct
    54001 ggctcgaaaa tgaaaccgta acagtgtaat aacaatgtga cacagagcac aaattatatt
    54061 tcgaatgaaa gtaaggatgg aattgatgat gtgaatgatt tggcccggcg acgccgccgc
    54121 cgggctgaga agattacagc atatgttcag tacgggcgat gatatcgtcc tgtgcgtctg
    54181 gagagagcgc ggtgaagaag gcggaatagc ccgcgacacg cactaccaga tcgcggtact
    54241 gatccggatg ttttttcgct tccagcagcg tttcgcggga aacgatgttg tactgaatat
    54301 gccagccttt atgcacttca aagaaggtac gcagcaggat catcagtttc tgcttgtcag
    54361 attcgttctc cagcgttgcc ggattcagtt tctggttgag caacacgccg ccgagaatcg
    54421 ctgccgtagg cagtttaccc actgagccaa tgactgccgt tgggccgaga tggtcagtac
    54481 cggaagccgg gcttgcgcct tctgccagcg gggtatgcgc tttacgtccg tccggcgttg
    54541 ccatcgtctg cgcgccaaac ggtacgttag cggagattga tgacgtaccc gcgtaatagt
    54601 tgccgccaac cggaccacga ccgtagcgcg gattatggta ctgtttcagt tcgtcgatat
    54661 aggtctgata agcgcgagcc agcagcatat cgacggtatc atcgtcgttg ccgtacttcg
    54721 gcgcaccgtt aatcagccgc tgacgcagtt gctcatgagt caggccttcg aagtcatccg
    54781 ccagtgcggc agccagctgt tgctgaccaa tcgcgccctg ttcaaacacc agtttcttca
    54841 ctgccgccag gctgttgccg aggttggcaa tgccgacctg caggccagaa acccagtcat
    54901 atttcgcgcc gccttgcttg atactttttg ctcgctcaat acagtcatcc accagcgccg
    54961 agcagagaat atcgtgcacg ttctcctcca gcatggtgtc aacgacatac tcaatttcga
    55021 ttgatttgcg ggtgtagtaa cggatttgcg tatcccacgc gtccatcact tcatcgaagt
    55081 tgttgaagtt acctgccgac aacgcttttt cttgtggcag gaacactttg ccgctggtgg
    55141 catcacgacc gccttccagc gccgccagca tcacgcgggc gaagttgata aagctcatgc
    55201 cggtacagcg atagccccat ttgccaccga cggcggtttc gatacaacca atcgctgcat
    55261 agtcgtaagc gtcctgcggt tcaataccga gtttaataaa ttccgggatc acgatttcgt
    55321 cgttgttgaa cgccggcatc ccgaagccgc aacggatcac ctgtacgcag gcgtcgagga
    55381 aatcgttgct cattcctgca tggtaacgca cgctgaggtt aggctgagtg gaacgcaggc
    55441 gaccgcagga ttcgaggatc gcgtaagaga gtggattcac cgcgtccatt ggttgaccat
    55501 caaccagatt ttgtccgcca atggtgacgt tctgatacag cggacttccc gcagaggctt
    55561 tcgagtgcga gccggagcgg attttgttca cttccagcag tttcagccag cagctatgca
    55621 gcatctcgat ggcgtgttcg cgatccagtg tctggttgag ttcaacgtcg cggcgatagt
    55681 acgggtagag atactggtcc atacgaccaa acgatactga gtgaccgtta gattcgatct
    55741 gcaaaatcaa ctggatgaag taacacagtt gcagcgcctg ccagaaagtc tgcggcggct
    55801 ggtgggcgat aagatcgcag ttttctgcca tcgccagcag ttcatcgcga cggctttcgc
    55861 gggtttcggt cgcggccatt tcacgcgcca gggcagcgaa acgttcaatg tgttcactga
    55921 ctgccaccag cacgatatca atcgctttca ggaattgttc gccgtgcaaa tcttccagca
    55981 ccgtcagatt gatgcgcgag cgacgttccg ctactttctc gcgcagacca tcaagccctt
    56041 tttccagcag tagcgggaaa ttgaccgcca ggtgtgcatc gccggaagtc atattgcctt
    56101 ccgctttaat gattccggtc gccagcagac ctttttgctc atcggtaaac atgccatagc
    56161 agcgatcctg tacggtctga ccgcgccacc acgggcacac ttcatgcaga acgcgtttgt
    56221 tctcttcgct caccgcaaag ccagcaccgg gacgatctgc cagatcatca atctcttttt
    56281 cgatccacga gacagtatat tccgggaaga tcggcgcggc gcgaacttcg cttgcctggt
    56341 tgccgatgat caactcatcg tgcttgatcc agatagtgcg attcgccagg tgatgtgcca
    56401 gcgccagcgc gcgacgtaca gggatcggct tatcgagatg ttgctgatac atctcggtat
    56461 agtgctgcgc gcgctcggta caaaccggtg gtttcacaat atgcaccagc gcatttttgt
    56521 gcgctttaat gcggtcgctg agcgtgtcca gtttcagtgt ggtcatggtt gttatcctcg
    56581 taaggtcgcg gttaaccctt tctggcaggc atactgctgg gcaaagtcga gcagttctgg
    56641 cgcatcaagc ggtttttccg gggcgtcata gggcagatta agtaagtgat atttgttgat
    56701 gcccagcgtg tggtagggca ggaaatgaat ttcgccaacg tgcagctcgt cggcggcaaa
    56761 atcggtaatg gcttttacag aggtttcgtc ggcattaaag ccctgaatca gtggcacgcg
    56821 gataatgatt tttttgcccg ctgcggcgag ttttttcagg ttatccagca ccctggcggc
    56881 gttaccgtcg gtccactgtt taaacggcgc gtcggcaacg tgttttaaat cggcaagaaa
    56941 cagatcgata tagggcagag aaggggcaat atatttccac ggcacatgca gacaggtttc
    57001 taccgcagta tgaatgccgg cctcgtggct ggcttgcagt agcgccgtcg ccatttccgg
    57061 ctgcataaag ggttcaccgc cagaaagtgt taagccgccg ccgctgcgat cgtaaaacgg
    57121 tttatcgcgc aaaacggtcg ccatgatctc ctcaacgctt ttcacttcac cacacacggt
    57181 taacgcctgt gtcgggcagc agtcggttag cgccgtcaga tgctccgggg ttaacttttc
    57241 ccgatgaata agcaaaccat tcaacgcgcg ctcaatcacg tccggcgcgg ccttagcgca
    57301 cagctcgcag ccttccagac acagtcgtgc gtcatacaac agatcctgcg tgcgggcgcg
    57361 gctttccggg ttctgacacc agcggcagcc cagcgaacag cctttaagaa atacgaccgt
    57421 gcggataccg gggccatcat gggtcgagta gcgctgaata ttgaaaatca tagttgcctc
    57481 tctgtttcat tcaaacatta aaatactttt gaatgaaagt tagattgatg tgcgtcaact
    57541 gttcagagag ttttcccgtg atagtctaca ttcagacaaa aagtacattt tgaggatggt
    57601 tatggaactg tatctggata cttcagacgt tgttgcggtg aaggcgctgt cacgtatttt
    57661 tccgctggcg ggtgtgacca ctaacccaag cattatcgcc gcgggtaaaa aaccgctgga
    57721 ggttgtgctt ccgcaacttc atgaagcgat gggcggtcag gggcgtctgt ttgcccaggt
    57781 aatggctacc actgccgaag ggatggttaa tgacgcgctt aagctgcgtt ctattattgc
    57841 ggatatcgtg gtgaaagttc cggtgaccgc cgaggggctg gcagctatta agatgttaaa
    57901 agcggaaggg attccgacgc tgggaaccgc ggtatatggc gcagcacaag ggctgctgtc
    57961 ggcgctggca ggtgcggaat atgttgcgcc ttacgttaat cgtattgatg ctcagggcgg
    58021 tagcggcatt cagactgtga ccgacttaca ccagttattg aaaatgcatg cgccgcaggc
    58081 gaaggtgctg gcagcgagtt tcaaaacccc gcgtcaggcg ctggactgct tactgacagg
    58141 atgtgaatca attactctgc cgctggatgt cgcacatcag atgattagct atccggctgt
    58201 cgatgctgcc gtggcgaagt ttgagcagga ctggcagggg gcgtttggca gaacgtcgat
    58261 ttaaccggac gttctgcatc ctcatcaatt gctgatgacg tggcagagtg caccgacgcc
    58321 tgccgcgcct ggaaatctgt gcccgaaccg taggccagat aaggcgttca cgccgcatct
    58381 ggcgtctgga gcaaatgcct gatgcgacgc ttgtcgcgtc ttatcaggcc tggatgtggc
    58441 aattactgca cgcacacttc acaccccgga ttgcgcatca gtttcatttc gcgaaactga
    58501 caggtcatcg catcgtacat gacgattttc ccgctggccg gtttgccata acctgccagc
    58561 agtttgatcg cttccattgc ctgcaacgaa ccaattacgc cgatcaacgg tgccatcacg
    58621 cctgcttcta cgcaggttaa cgagttctcg ccaaacaaac ggctgaggca gcgatagcaa
    58681 ggctcgccgt cctgataagt gaagaccgtg atttgacctt ccatacgaat tgccgcgccg
    58741 gaaaccagcg gcaccttcgc ggcaaaacag cctgcgttca gttgattacg taccgcaacg
    58801 ttatccgtac agtcgagcac cagatcgtgt tcagcaatca atgctgcaag ttctgcgtca
    58861 tccagcagtg cattgactgg cgtaatcgca atatgtgggt tgatccgcgt cagggcgtca
    58921 cgggcggatt ctaccttcgg ttgcccgacc gtagcatcac tgtgcagcgt ctggcgttgc
    58981 agattcgaga gcgaaaccgt gtcgaagtcg agcagcgtca gggtgccgac accggcgctt
    59041 gccagatact gcgaggctgc gcagccgagt ccaccgaggc cgactaccag cacacgagaa
    59101 tcttttagcg cctcctgacc gtcaaaatca aagccgcgca gaatgatttg ccggttgtag
    59161 cgcagcatct cctgatcgct gagttccgcc attacaggcc tccgaacaac gcgttaaacg
    59221 gttctacttc cacccattcg cccacttcca cattgccgcg atcgcgttcc agcacgataa
    59281 agcagttgcc gaggctaaag gagctaaata tatgtgaacc ctgatgtccg gtggtcgtca
    59341 cttccagttc gccatcggcg ttgcgttgca gcacgccgcg ctggaaatca aggcgacctg
    59401 gcgttttttt caggcgggac gctgtgcgta cgcgctggcg cgcgggcagg ccgctggcgg
    59461 tgttaccgct tagttttgcc agcaaaggct gtaccagttg atagaaggtc agcgtcgctg
    59521 aaaccgggtt gcccggcagg ccgcagaacc agctattgct gagtttaccg aacgcgaacg
    59581 gtttacctgg tttaatcgcc agcttccaga aggcgatctc ccccagctct tcaagaatcg
    59641 ttttggtgta atccgcttca cccactgaaa caccgccgga actgatcacc acatccgcct
    59701 ggctgtcggc ttcaataaat gcggcgcgca gggcatgtgg atcgtcgcgg ataatcccta
    59761 agttgattac ctcgcatccc aactgttcta acatcaggtg tacggcgaga cggttggtat
    59821 cgtagatttg gccgtcgccc agcggctgac cgggcaactg gagttcatca ccggtagaaa
    59881 aaagcgctac acgcacttta cgaatcaccg gaacttcggc aatccccagt gaagcaatta
    59941 ctggcagctc tgcggtagtc aggcgagttc ccgccgggaa aacaaccgca cctgcagaga
    60001 tatcttcacc gcgacggcga atattttgcc cgctacgcac ttcagcagta aaacgcacgc
    60061 cattgtccgt ttgttcagtc tgttcctgca tcaccaccgc ttcgcagcct tcgggtactg
    60121 gtgcgccagt cataatgcgg atgcaggtgc cagcgggcca ctcgccatgg taaggctgac
    60181 cggcaaacga cttcccggca acgggcagcg gttgtccgga ggtgatatcg gccaaacgta
    60241 ccgcgtaacc gtccattgcg gagttatcaa accctgggac atccagcggc gaaacgacat
    60301 cgctcgccag aatacgtcca aaacactgaa ccagtggcag cgtttcctgg gcggtcagtg
    60361 gggtgacgcg agaaagcatc tcattaagcg cggtgtcgag cgacatcaat ccggtggtaa
    60421 attccatgaa aacactcctg cggaggcaaa atcgaatttg cctattatgt cagaaaaacg
    60481 ccacagactg tatgccacct cgggcgtagc gct
//
LOCUS       DAZFZC010000018        59638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFZC010000018 DAZFZC010000000
VERSION     DAZFZC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.018"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..110
                     /locus_tag="ACHKXP_001321"
     rRNA            <1..110
                     /locus_tag="ACHKXP_001321"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            205..320
                     /gene="rrf"
                     /locus_tag="ACHKXP_001322"
     rRNA            205..320
                     /gene="rrf"
                     /locus_tag="ACHKXP_001322"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            646..1944
                     /gene="kgtP"
                     /locus_tag="ACHKXP_001323"
     CDS             646..1944
                     /gene="kgtP"
                     /locus_tag="ACHKXP_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO7646374.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1941..2264)
                     /gene="yfiM"
                     /locus_tag="ACHKXP_001324"
     CDS             complement(1941..2264)
                     /gene="yfiM"
                     /locus_tag="ACHKXP_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO7646375.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2310..3665)
                     /gene="pssA"
                     /locus_tag="ACHKXP_001325"
     CDS             complement(2310..3665)
                     /gene="pssA"
                     /locus_tag="ACHKXP_001325"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO7646376.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3779..6439)
                     /gene="pat"
                     /locus_tag="ACHKXP_001326"
     CDS             complement(3779..6439)
                     /gene="pat"
                     /locus_tag="ACHKXP_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO7646377.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6471..7169)
                     /gene="tapT"
                     /locus_tag="ACHKXP_001327"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6471..7169)
                     /gene="tapT"
                     /locus_tag="ACHKXP_001327"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO7646378.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7238..7657)
                     /gene="trxC"
                     /locus_tag="ACHKXP_001328"
     CDS             complement(7238..7657)
                     /gene="trxC"
                     /locus_tag="ACHKXP_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO7646379.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7864..8901
                     /gene="yfiF"
                     /locus_tag="ACHKXP_001329"
     CDS             7864..8901
                     /gene="yfiF"
                     /locus_tag="ACHKXP_001329"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7646380.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8949..9638)
                     /gene="ung"
                     /locus_tag="ACHKXP_001330"
     CDS             complement(8949..9638)
                     /gene="ung"
                     /locus_tag="ACHKXP_001330"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO7646381.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9943..10326
                     /gene="grcA"
                     /locus_tag="ACHKXP_001331"
     CDS             9943..10326
                     /gene="grcA"
                     /locus_tag="ACHKXP_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO7646382.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10382..10969)
                     /gene="eamB"
                     /locus_tag="ACHKXP_001332"
     CDS             complement(10382..10969)
                     /gene="eamB"
                     /locus_tag="ACHKXP_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO7646383.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSTTSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDVLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            11072..11953
                     /gene="yfiE"
                     /locus_tag="ACHKXP_001333"
     CDS             11072..11953
                     /gene="yfiE"
                     /locus_tag="ACHKXP_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7646384.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRSTVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(12162..13496)
                     /gene="srmB"
                     /locus_tag="ACHKXP_001334"
     CDS             complement(12162..13496)
                     /gene="srmB"
                     /locus_tag="ACHKXP_001334"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO7646385.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13628..14365
                     /gene="trmN"
                     /locus_tag="ACHKXP_001335"
     CDS             13628..14365
                     /gene="trmN"
                     /locus_tag="ACHKXP_001335"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO7646386.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYEQGVECATPQREQARYTTSLDHQTLLTCAAECIT
                     EEGFFCVVLPEQIGKSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14350..15972)
                     /gene="nadB"
                     /locus_tag="ACHKXP_001336"
     CDS             complement(14350..15972)
                     /gene="nadB"
                     /locus_tag="ACHKXP_001336"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO7646387.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRMADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMIRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            16228..16383
                     /gene="rseD"
                     /locus_tag="ACHKXP_001337"
     CDS             16228..16383
                     /gene="rseD"
                     /locus_tag="ACHKXP_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO7646388.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16380..16955
                     /gene="rpoE"
                     /locus_tag="ACHKXP_001338"
     CDS             16380..16955
                     /gene="rpoE"
                     /locus_tag="ACHKXP_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO7646389.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16988..17638
                     /gene="rseA"
                     /locus_tag="ACHKXP_001339"
     CDS             16988..17638
                     /gene="rseA"
                     /locus_tag="ACHKXP_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO7646390.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNLEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17638..18594
                     /gene="rseB"
                     /locus_tag="ACHKXP_001340"
     CDS             17638..18594
                     /gene="rseB"
                     /locus_tag="ACHKXP_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO7646391.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            18591..19070
                     /gene="rseC"
                     /locus_tag="ACHKXP_001341"
     CDS             18591..19070
                     /gene="rseC"
                     /locus_tag="ACHKXP_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO7646392.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            19268..21067
                     /gene="lepA"
                     /locus_tag="ACHKXP_001342"
     CDS             19268..21067
                     /gene="lepA"
                     /locus_tag="ACHKXP_001342"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO7646393.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETMSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            21083..22057
                     /gene="lepB"
                     /locus_tag="ACHKXP_001343"
     CDS             21083..22057
                     /gene="lepB"
                     /locus_tag="ACHKXP_001343"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO7646394.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            22329..23009
                     /gene="rnc"
                     /locus_tag="ACHKXP_001344"
     CDS             22329..23009
                     /gene="rnc"
                     /locus_tag="ACHKXP_001344"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO7646395.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            23006..23911
                     /gene="era"
                     /locus_tag="ACHKXP_001345"
     CDS             23006..23911
                     /gene="era"
                     /locus_tag="ACHKXP_001345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO7646396.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23923..24651
                     /gene="recO"
                     /locus_tag="ACHKXP_001346"
     CDS             23923..24651
                     /gene="recO"
                     /locus_tag="ACHKXP_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO7646397.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIESLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24663..25394
                     /gene="pdxJ"
                     /locus_tag="ACHKXP_001347"
     CDS             24663..25394
                     /gene="pdxJ"
                     /locus_tag="ACHKXP_001347"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO7646398.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25394..25774
                     /gene="acpS"
                     /locus_tag="ACHKXP_001348"
     CDS             25394..25774
                     /gene="acpS"
                     /locus_tag="ACHKXP_001348"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO7646399.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            26195..26275
                     /gene="shoB"
                     /locus_tag="ACHKXP_001349"
     CDS             26195..26275
                     /gene="shoB"
                     /locus_tag="ACHKXP_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO7646400.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(26469..26729)
                     /gene="yfhL"
                     /locus_tag="ACHKXP_001350"
     CDS             complement(26469..26729)
                     /gene="yfhL"
                     /locus_tag="ACHKXP_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO7646401.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26785..27633)
                     /gene="yfhH"
                     /locus_tag="ACHKXP_001351"
     CDS             complement(26785..27633)
                     /gene="yfhH"
                     /locus_tag="ACHKXP_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO7646402.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLATDEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27842..28477
                     /gene="yfhb"
                     /locus_tag="ACHKXP_001352"
     CDS             27842..28477
                     /gene="yfhb"
                     /locus_tag="ACHKXP_001352"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO7646403.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARRPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28535..29038
                     /gene="tadA"
                     /locus_tag="ACHKXP_001353"
     CDS             28535..29038
                     /gene="tadA"
                     /locus_tag="ACHKXP_001353"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO7646404.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(29035..30591)
                     /gene="mltF"
                     /locus_tag="ACHKXP_001354"
     CDS             complement(29035..30591)
                     /gene="mltF"
                     /locus_tag="ACHKXP_001354"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO7646405.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30849..34736
                     /gene="purL"
                     /locus_tag="ACHKXP_001355"
                     /gene_synonym="purI"
     CDS             30849..34736
                     /gene="purL"
                     /locus_tag="ACHKXP_001355"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO7646406.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELREILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAVQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADRKAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DATHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            35294..36721
                     /gene="qseE"
                     /locus_tag="ACHKXP_001356"
     CDS             35294..36721
                     /gene="qseE"
                     /locus_tag="ACHKXP_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO7646407.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLHNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36886..37599
                     /gene="qseG"
                     /locus_tag="ACHKXP_001357"
     CDS             36886..37599
                     /gene="qseG"
                     /locus_tag="ACHKXP_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO7646408.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            37589..38923
                     /gene="glrR"
                     /locus_tag="ACHKXP_001358"
     CDS             37589..38923
                     /gene="glrR"
                     /locus_tag="ACHKXP_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO7646409.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSAVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38984..39322
                     /gene="glnB"
                     /locus_tag="ACHKXP_001359"
     CDS             38984..39322
                     /gene="glnB"
                     /locus_tag="ACHKXP_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO7646410.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(39367..40557)
                     /gene="hmpA"
                     /locus_tag="ACHKXP_001360"
     CDS             complement(39367..40557)
                     /gene="hmpA"
                     /locus_tag="ACHKXP_001360"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO7646411.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40885..42138
                     /gene="glyA"
                     /locus_tag="ACHKXP_001361"
     CDS             40885..42138
                     /gene="glyA"
                     /locus_tag="ACHKXP_001361"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO7646412.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            42178..42276
                     /locus_tag="ACHKXP_001362"
                     /pseudo
     CDS             42178..42276
                     /locus_tag="ACHKXP_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(42336..43529)
                     /gene="yphH"
                     /locus_tag="ACHKXP_001363"
     CDS             complement(42336..43529)
                     /gene="yphH"
                     /locus_tag="ACHKXP_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7646413.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDHTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43647..46928
                     /gene="yphG"
                     /locus_tag="ACHKXP_001364"
     CDS             43647..46928
                     /gene="yphG"
                     /locus_tag="ACHKXP_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO7646414.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQACELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            47025..48008
                     /gene="yphF"
                     /locus_tag="ACHKXP_001365"
     CDS             47025..48008
                     /gene="yphF"
                     /locus_tag="ACHKXP_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO7646415.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            48031..48264
                     /locus_tag="ACHKXP_001366"
                     /pseudo
     CDS             48031..48264
                     /locus_tag="ACHKXP_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            48265..48405
                     /locus_tag="ACHKXP_001367"
                     /pseudo
     CDS             48265..48405
                     /locus_tag="ACHKXP_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
     gene            complement(48453..48875)
                     /gene="yphA"
                     /locus_tag="ACHKXP_001368"
     CDS             complement(48453..48875)
                     /gene="yphA"
                     /locus_tag="ACHKXP_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7646416.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(48972..50174)
                     /gene="hcaD"
                     /locus_tag="ACHKXP_001369"
     CDS             complement(48972..50174)
                     /gene="hcaD"
                     /locus_tag="ACHKXP_001369"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO7646417.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(50184..50996)
                     /gene="hcaB"
                     /locus_tag="ACHKXP_001370"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(50184..50996)
                     /gene="hcaB"
                     /locus_tag="ACHKXP_001370"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO7646418.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(50993..51313)
                     /gene="hcaC"
                     /locus_tag="ACHKXP_001371"
     CDS             complement(50993..51313)
                     /gene="hcaC"
                     /locus_tag="ACHKXP_001371"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO7646419.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(51313..51831)
                     /gene="hcaF"
                     /locus_tag="ACHKXP_001372"
     CDS             complement(51313..51831)
                     /gene="hcaF"
                     /locus_tag="ACHKXP_001372"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO7646420.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(51828..53189)
                     /gene="hcaE"
                     /locus_tag="ACHKXP_001373"
                     /pseudo
     CDS             complement(51828..53189)
                     /gene="hcaE"
                     /locus_tag="ACHKXP_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
     gene            53325..54215
                     /gene="hcaR"
                     /locus_tag="ACHKXP_001374"
     CDS             53325..54215
                     /gene="hcaR"
                     /locus_tag="ACHKXP_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO7646421.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54375..55514
                     /gene="hcaT"
                     /locus_tag="ACHKXP_001375"
     CDS             54375..55514
                     /gene="hcaT"
                     /locus_tag="ACHKXP_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO7646422.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(55506..56786)
                     /gene="csiE"
                     /locus_tag="ACHKXP_001376"
     CDS             complement(55506..56786)
                     /gene="csiE"
                     /locus_tag="ACHKXP_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO7646423.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(56977..57831)
                     /gene="yfhR"
                     /locus_tag="ACHKXP_001377"
     CDS             complement(56977..57831)
                     /gene="yfhR"
                     /locus_tag="ACHKXP_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7646424.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKLYGP
                     DPWSAESVEFTAKDGTRLQGWFIPFSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYHGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(57976..58779)
                     /gene="suhB"
                     /locus_tag="ACHKXP_001378"
     CDS             complement(57976..58779)
                     /gene="suhB"
                     /locus_tag="ACHKXP_001378"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO7646425.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            58898..59638
                     /gene="trmJ"
                     /locus_tag="ACHKXP_001379"
     CDS             58898..59638
                     /gene="trmJ"
                     /locus_tag="ACHKXP_001379"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO7646426.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
BASE COUNT        14344 a        15047 c        15902 g        14345 t
ORIGIN      
        1 ctttaagggt cctgaaggaa cgttgaagac gacgacgttg ataggccggg tgtgtaagcg
       61 cagcgatgcg ttgagctaac cggtactaat gaaccgtgag gcttaacctt acaacgccga
      121 agctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
      181 agcggtctga taaaacagaa tttgcctggc ggccgtagcg cggtggtccc acctgacccc
      241 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
      301 agagtaggga actgccaggc atcaaattat gcgaaaggcc atcctgacgg atggcctttt
      361 tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
      421 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
      481 ccagaaattt atccttaagc tcctcaataa ccattttcct gctaactaaa ttcatggtta
      541 aggttgcata atgatatgca acaaatgtat aacatttcct ttaccaaaaa aaataaacaa
      601 aagcgaccga caaaagcatc ggattacggc aggagacata atggcatggc tgaaagtact
      661 gtaacggcag acagcaaact gacaagtagt gatactcgtc gccgcatttg ggcgattgtg
      721 ggggcctctt caggtaatct ggtcgagtgg ttcgatttct atgtctactc gttctgttca
      781 ctctactttg cccacatctt cttcccttcc gggaacacga cgactcaact actacaaaca
      841 gcaggtgttt ttgctgcggg attcctgatg cgcccaatag gcggttggct atttggccgc
      901 atagccgata aacatggtcg caaaaaatcg atgctgttat cggtgtgtat gatgtgtttc
      961 ggatcgctgg ttatcgcctg cctcccaggt tatgaaacta taggtacgtg ggctccggca
     1021 ttattgcttc tcgctcgttt atttcaggga ttatctgttg gcggagaata tggcaccagc
     1081 gccacctata tgagtgaagt tgccgttgaa gggcgcaaag gtttttacgc atcatttcag
     1141 tatgtgacgt tgatcggcgg acaactgcta gccctactgg ttgtcgtggt tttacaacac
     1201 accatggaag acgctgcact cagagagtgg ggatggcgta ttcctttcgc gttaggagct
     1261 gtgttagctg ttgtggcgtt gtggttacgt cgtcagttag atgaaacttc gcaacaagaa
     1321 acgcgcgctt taaaagaagc tggatctctg aaaggattat ggcgcaatcg ccgtgcattc
     1381 atcatggttc tcggttttac cgctgcgggc tccctttgtt tctatacctt cactacttat
     1441 atgcagaagt atctggtaaa tactgcggga atgcatgcca acgtggcgag tggcattatg
     1501 actgccgcat tgtttgtatt catgcttatt caaccactca ttggcgcgct gtcggataag
     1561 attggtcgcc gtacctcaat gttatgtttc ggttcgctgg cagccatttt taccgttcct
     1621 attctctcag cattgcaaaa cgtttcctcg ccttatgccg cttttggtct ggtgatgtgt
     1681 gccctgctga tagtgagttt ttatacatca atcagtggaa tactgaaggc tgagatgttc
     1741 ccggcacagg ttcgcgcatt aggcgttggt ctgtcatatg cggtcgctaa tgctatattt
     1801 ggtggttcgg cggagtacgt agcgttgtcg ctgaaatcaa taggaatgga aacagccttc
     1861 ttctggtatg tgaccttgat ggccgtggtg gcgtttctgg tttctttgat gttacatcgc
     1921 aaagggaagg ggatgcgtct ttagtgacgg gtcagttgcc agacggtata gccggtgctt
     1981 gcaccggcga catcccaggc caaatccttc cagctccagc cgctcccttc ggggcggcta
     2041 tcccaaagct cttttgacgc ccccaaactg acagagaaca tcaatccaaa catggcactg
     2101 cgatcccggc tcatcccctg atgctgtgaa tattcatttc cggcggcgga aagcatcgcc
     2161 gaggcgataa agtgttgagc tttatcctgc ccgctccagc tatcgttagc catatggctg
     2221 catccagaaa gtaacaatag cgaacagaca aaaagaatac gcaaaacgtg gcctccaaaa
     2281 aacaaacccc gtacaggacg gggttgtgat tacaggatgc ggctaattaa tcggtcgatg
     2341 cggatacggc gcaaacggcg gatgagttta cgaaccttca ccggataatc ggcaatactt
     2401 tgcagatcgc gatagtgctt aacgatggtg gtatgctcgc ggatcagctc cagttctttc
     2461 tctcgctgtg gcgccagctc aagttgcgga tcgtggatca aaatggcgtt ttccagatcc
     2521 agacgccagg cgcgcgggtt caggttatta ccggtgatca acatccactt atcatcaacc
     2581 cacatccctt tcaggtgata ggtgttgtcg tcatctttcc ataaccgaac cactagctgg
     2641 tcagtattga cgtaatactg caaacggctc aggaaacgac gcagattgat ctcatagaga
     2701 taaggcaatg cgccaattat cttgaaaggt tcatcttccg gaatgtagaa gtcattcgcg
     2761 gttttatcac caacaataat ttcgaccttt ttcccttcgc gcagcaactg gataatattg
     2821 cgcacaagga ttgctggcag gttgaagtat ggcgtacaga tggttagttt ctgctcggca
     2881 caaggcataa gatggaaaat ggtcttgttc aacagactcg atttccccag ccccactagc
     2941 ggcgttacag aaagctgatc gttgtcggca tcgccctgga aatgataagc ggcatcacgc
     3001 agctcctggc ggaacagacg aatatcgttc ttgatttccg ggctttttgg ccgattaaca
     3061 tcatccagac gattaacgcc gcggccattc ataatattct gtgtaaccca ttcaaacata
     3121 atgtctgaca tcttacggtt acggatcaga tgataacggt cgtagcgata tttatcgtgc
     3181 tgatgcaggt aaacatcgtt caggctggca ccgctataaa gtacgctatc gtcgatgata
     3241 aagcctttaa agtgcagaac accaagggct tcacgagtat tgattggaac gccataaacc
     3301 ggaacatcta cgcccggatt ttcctgcgcc atgcggcagt accagtcagc gttagtgtta
     3361 gatgccgcag cgccaatgcg tccacgttgt gcacgatgcc agtcgaccag cacccgcaca
     3421 tccagttccg gacgctgcct tttagcctca tacaacgcgt tcagaatgcc tttgccaccg
     3481 tcatcctgtt cgagatacag ggcgacaatg caaatgcgct gcttcgcgct ggctattttt
     3541 tccagcagcg tctcccggaa gtcggcggga gcgtaaaaga aatcgacatc atcaactgat
     3601 tgagaaatct tgggtagttg ggcaaggtgt tgttgatgtt tattacgctt aaattttgac
     3661 aacatcacag tgcatttctt ctctgttcat tgaagggtcc tctgtgcaat gcagacgaca
     3721 taagcgggca ataataacac cagtcccgat taagtggtca acatttccag taccttactc
     3781 atgattcctc gcgctgggca agatttagcg taagcccaac gatcccctct tcgagctgga
     3841 tatcaacgtt aaacccgagc ttgcgggcta gcgccaccat gccacgattg tttggcatcg
     3901 taataccatt cagacgttgt agtccgtgat ctcgcgtata ggtaatcaac ttttccatta
     3961 agcgtcgacc taagcctaac cctttgagat ccgagcgaac cagtacagca aattcggcat
     4021 cgatgttatc aggatcggaa atcgcacgcg tgacgccgag gatctcttcc gtttgatcaa
     4081 tacgtcgtac cgctacaaac gccatttccc gatcgtagtc gatctgtgtc atgttggcta
     4141 aatcttcatg ggtaaattcg ttgatctcgc taaagtagcg gtaataaaga tcttctttgg
     4201 tgactcgcga aatgaattgc tgaagttgtg gctcatcttc tggcaaaatc gggcggaaca
     4261 agcagcgttc accgtttttc aattctaccc attcttccag ctgatgcgga taagggcgca
     4321 ctgccagccg actctcgtta tcgccttcaa acggcgagat atccagcgtg acatccagcg
     4381 cggtaaattc actgccagaa gccagcaaag gatgaatatc cagacgctga atttccgggc
     4441 aatcgacaat caagttggaa acctgcacca gaagctggct caagcctgca acatccaatg
     4501 ggcgtagcgc actgcgcgca cgaatctttt tacttttgat cccctgaata accagatagc
     4561 gggccaggtt catgttcagc ggcggcagtg cgacgacggc ttgatcttca ggacgccact
     4621 ccacaccgcc ttcacccagc atgatcaacg gcccgaaaac cggatcgtgc tcaaccacaa
     4681 cccgcaactc ctgagcgcca gcacggttag ccatactttg caccaacagg ccgtggaccc
     4741 gcgcctgtgg ccaggccatt tttacgcgat cgaaaatagc gttcgccgct tgctggactt
     4801 cattggctgt acgcaggtaa agcatgacgc cctgaacttc cgatttatgt ggaatatccg
     4861 gcgaacgcaa tttcagcgcc accggataac caatctgttc ggcaatatgc accgcttcgg
     4921 tgctatcgct ggcaatccag gtagggagcg tgttcatgcc atacgcttgc aggatgggct
     4981 gaacttcatg ggtatcgagc gacgtagccc cttcggcaat cgcctgttgc aacagaagat
     5041 gcgcttctgc ggtattggaa gtcagattgc tgggcaacgc cggcgtttcg cgtagttgct
     5101 tctgattacg ccggtactcc accatatgca taaaagcagt gatggttcct tccggggtac
     5161 ggtaggtcgg cagcccggct tcgctgaata aacgtcgtgc ctcttgcgag gagtgctcgc
     5221 cgcaccagtt cgtcagcaaa gagacatatt tgctgcgggg atgatgcttt accgcttcaa
     5281 ttaatacttg cgcgctttct gttgcgggag cagcggcgct gggcgaatga ataaccatca
     5341 gcgcgtcaaa atcctggctg tggagcagaa tatccagcgt tttaatatag tgctcactgc
     5401 tggcgtcatc gcgtagatcg agcgggttag atattgccac atgttctggc agtgcatcgc
     5461 gcagtttctg gcaggtttct tcgcttagcg ttgccagctt gccattgcgt gaccataagg
     5521 catccagcgc cagcgcggca ggcgcagcac cgttgctgat aatcatcagc cggtcgccac
     5581 gtagcgggcg catatggcta agggtttcca ccgccgaaaa cagctcgtgg gtgtcctgta
     5641 cccgcaacaa accggcacgc tgaatagccg catcccatgc cgggtccatt cctgccgtcg
     5701 tgttgagcag tcgctgtgcc gccgggctac gtccgctttt aatcaccaga atcggtttat
     5761 tacgcgaggc actacgggcc gccgaaacaa agcgtcgcgc gtcgcttaac tgttcgagat
     5821 agagcaggat ggcgctggtt ttactgtcgc gtgccagata gtcaagcaat tcatcaacgt
     5881 cgatatccag gctgtcgccg agcgcaataa agtaggaaaa gcccatctta cgctgttgcg
     5941 cccagtcgag gatggtgttg gagacggcag ccgattgcga aataaacgcc agcttgccgc
     6001 gtttaatcgg cacaggcgaa aagctggcat tcagaccttg ccagggagcc agtaaaccca
     6061 gactgtttgg tccaagcagg cgcatgttat ggcgcagggc gcaggcgcgg agatcttcgt
     6121 gttgcgatgc cggggcggaa agaataatgc aggttttaca gcctttctcg ccgagctctt
     6181 ccagaagagc aagattacgg ctggcattgg tacataaaac cgcaaggtcg ggtgtaaagg
     6241 gcaagctggc aatatccggc caggccaaca cacccaacac cgctttccag gctggcgtca
     6301 ccgggagtac cggtccgtta aagcctcccg ccagcaggtt acgcatcatc aggtaacctg
     6361 cgcgattggg tttcatcgac gcgccaatta ccgctatcga ttttggtcgc agtagtgctt
     6421 ccagtcctcg ctgactcata ccggtctccc gcagaagtga ccgaatgatt ttaaacgctt
     6481 tctaactgtt ctgctgtgat gctacccaga tgttgcgttt ttcctgccag atagcgtgtt
     6541 ttaaagcggg taaaatgctc gcctaaccct gccgccgccc cggtatcgcc ggccatatct
     6601 aacagtgcga tggctacctc ggcagtacaa tattggcctt cagcctgggc ttcacgcagg
     6661 cgataggcag aaagccggga aagatcgacg gaaatgacgg gaagattatc cagatacgga
     6721 cttttacgaa acatcttgcg agcttccggc caggtaccat cgagcatgat aaacagcggt
     6781 ggcttaccgg caggtggtgt gaagatcact tcccgttgct catcagcata cgaagcggga
     6841 aagaccacca ttggctgata gtccgggttt tgcaccaact ccagcaaatc ctgcgagggt
     6901 tcggtacgcg accattgaaa cgcaacggta tcaggcaaaa tatcagcaat gagacgcccg
     6961 gtattactgg gcttcattgg ctcggtgtcg aacatcagca aacagaagcg actttttgct
     7021 tgtgctgggg taattgtcga acagagacat aatttctctg gcaaaagaca gcgttggcag
     7081 cgacgaacgc gattaccgcg ggcaagaaaa ggacgtgttg cgcgcgcaat acgctcggcg
     7141 cgtaactgga gaacagcgtt ttcggtcata agagagcgtc gaaaaaacgc cattgtcgca
     7201 gaggagaaaa cggggcacaa gatgcgcccc ggtaagatta aagagattcg ttcagccagc
     7261 tatcgaacgg cgctttcggt actgcgccat taagcatgtc gacaacctga ccgtttttga
     7321 aaatcatgat cgtcggtata ctacgaattc caaagcgact gctcaattca cgttcagctt
     7381 cggtattcac tttcacaaag cgcactttac cgctacgctc ttgcgcgaca tcttcaaaaa
     7441 ttggtgcgaa attacggcag gggccgcacc acggtgccca gaagtcgatc accacaggta
     7501 gatcatcctt cagcaatttg tcgagtgttt caccggtcgc attaatcacc tctccgtcaa
     7561 acaagtcgtg accgcagcgt ccgcattttg ccgcatcttc gatccgatcg tcgggaatgc
     7621 gattgatggc ctgacaatgg gtacaaacgg tattcataac taacctcggg atgagtaagg
     7681 agacttatcg gcaacgacgc cggtatgttt ctaatatgtt acatattatc ggggacattg
     7741 ttccagacaa caatctgttt tattcaatag gtatgatagt cgcaggcttt ggtacgaaat
     7801 catggcgaac cgcgcggact tcgggtaatc tgcgcgcttc gcgcagcgct ggtggagaaa
     7861 agcatgaacg atgaaatgaa aggtaaaagc ggcaaggtca aagtgatgta tgtccgcagt
     7921 gatgatgatt ctgataaacg tacccacaac ccgcgtaccg ggaaaggggg cgggcgtcca
     7981 ggaaaatctc gtgctgacgg tggccgtcgc cccgcccgcg atgacaaaca gagtcagccc
     8041 cgtgaccgca agtgggaaga ttcgccgtgg cgcacggttt cccgcgcgcc gggtgatgag
     8101 acgccggaaa aggccgatca cggtggcatc agtggtaaaa gttttattga tccagaagtt
     8161 ttgcgtcgtc agcgtgcgga agaaacccgc gtctacggcg aaaatgcatg tcaggcactg
     8221 ttccagagcc gtccggaagc gattgttcgc gcctggttta tccagagcgt aacgccacgt
     8281 tttaaagaag ccttgcgctg gatggcggca aaccgcaaag cgtaccatgt ggtggatgaa
     8341 gcggaattga caaaagcgtc aggcacggaa catcacggcg gcgtttgctt cctgatcaaa
     8401 aagcgtaatg gcacaaccgt gcagcagtgg gtaagccagg caggtgcgca ggattgtgtt
     8461 ctggcactgg aaaacgaatc taacccgcat aacctgggtg gcatgatgcg cagctgcgcg
     8521 cacttcggcg tgaaaggtgt tgtggtgcag gatgcggcac tgctggagtc gggtgcggct
     8581 atccgtaccg cagaaggcgg cgcagagcac gttcagccga ttactggcga caacattgtt
     8641 aacgtgctgg atgatttccg tcaggcgggt tacaccgtag tgacaacttc cagcgagcag
     8701 ggtaaaccgc tgttcaaaac cagtctgcca gcgaaaatgg tactggtgct gggtcaggaa
     8761 tatgaagggt taccggatgc cgcacgcgat ccgaacgatc tgcgcgtgaa gattgatggt
     8821 actggcaacg ttgccggact gaatatctct gtcgcaactg gcgttttgct tggtgaatgg
     8881 tggcgtcaga ataaagcctg ataaatcagc cgggtggcaa cgctgccatc cggcattttc
     8941 ccgcaaattt actcactctc tgccggtaat actggcatcc agtcaatcgg cgtctcgcca
     9001 cgttgttcca gccactgatt tgccagcaca aaatggttac agccaaagaa tcctctatgt
     9061 gccgaaagcg gcgacggatg cggtgctttc agtacatgat ggcgttgctt atctataatc
     9121 gcccctttct tttgcgcatg tgatccccac aacaaaaaca ccacgccttc gcgatgctgg
     9181 ttaatcaggc tgatcacttt atcggtgaag gtctcccagc cgaggctggc gtgggaatgc
     9241 gcctgacctg cgcgtaccgt caacacagta ttgagcagta gaacgccctg acgcgcccaa
     9301 ctttcaagat aaccatgatt agggcgggtg aagcccggaa tagtattttc cagctcttta
     9361 tacatattca gcagtgacgg aggagtggcg attccggggc gaacggaaaa tgccagacca
     9421 tgcgcctgtc ccggtccgtg ataaggatcc tggccgagaa tcaccacttt aacgtcaccc
     9481 aactcggtaa agcggaacgc gttaaagaca tctttttgtg gtggatagat agtgacgccg
     9541 gactgccgct cgctggcgac ggtctgaagg gtattaagaa aataaggttg ttgcttctct
     9601 tcagccagca cgtcatgcca ggttaattcg ttagccatct cgctctcctg cgaatcttca
     9661 atccgcctag cttaactgct tctttctaca gaacaaaatc atgctcattt tttagactta
     9721 aacgagtgca aaaaaatgta aattggtaaa atttcacacc cgcataagtt gatgtaaaac
     9781 aataaaatcc atttgtgacc acaatcgatg tgtggttttt attgatttaa atcaaagaat
     9841 caagggtggt tgaggagtat atatacactc aagcaacaat ggttttacca attggccgcg
     9901 acaggctgaa caaatcaaat aattttgccg gggaggcatc acatgattac aggtatccag
     9961 attactaaag ccgctaacga cgatctgctg aactctttct ggctgctgga cagcgaaaaa
    10021 ggcgaagcac gttgcatcgt tgcaaaagca ggttatgcag aagatgaagt ggttgcagta
    10081 agcaaactgg gtgacattga ataccgtgaa gttccagtag aagtgaaacc agaagttcgc
    10141 gttgaaggtg gtcaacacct gaacgttaac gttctgcgtc gtgaaactct ggaagatgca
    10201 gttaagcatc cggaaaaata tccgcagctg accatccgtg tatccggata tgcagttcgc
    10261 tttaactctc tgactccgga acagcagcgc gacgttattg ctcgtacctt tactgaaagc
    10321 ctgtaaggct taagtagcaa gttattaagc ggaagagggc gacttcttcc gctttttttc
    10381 gttaatagaa aatgcgtacc gcgcaataga ccagcaacag tgcaagcacg atatttaact
    10441 ggcgaccata ctggcgaaac aatcgctgaa acagatgccc cgccagcgcc cagcacacat
    10501 tgccaaacgt cccaatcatc gccagcaaaa cgctgacgcc aactacccag cttagcgcct
    10561 gtgtttgcgg cagaacaaac gtcgacagtg ccgtaacacc gtacaaaatg attttgacgt
    10621 tcacaaactg caaagcaaag ctggcccaaa agctgattgg ttttgcctga agtacgtctt
    10681 cctttgttgg gctggtggcg attttccacg ccagccagac aatatatgcc gcccccgccc
    10741 aactcaaaag gtgtaccgct gccgggtcaa tcactgccag tgaaaatgaa atgcccgcac
    10801 acagtaacat cacaatcaaa aatcccagac tcatccctgc cagcacgcgg gtactttgac
    10861 gaaatccatg cgacgtagta gagctaaggg cgagaatatt gttcggtcct ggcgtcatgg
    10921 cggtaatcag ggtgtaagtc caaaaagcac ttaaaagggt cggtgtcact tgttatctcc
    10981 tctgttttgg tagacagtac ccgacacgga acatttaaaa aaatgaatgt ttttacattc
    11041 taaattcgat attttcgata ggtttggggt tatggatctg cgccgtttta ttacacttaa
    11101 aaccgtggtg gaagagggtt cctttttgcg agcttcgcaa aaattgtgct gtacacaatc
    11161 gacggtgact tttcatattc agcagcttga gcaggagttt tcagtccagt tatttgagaa
    11221 aattggccga cggatgtgcc ttacccgcga aggaaaaaag ttgctgccgc acatttatga
    11281 acttacccga gtgatggata cgcttcgtga ggctgccaaa aaggagtcag atccggacgg
    11341 ggaactacgc gttgtttcgg gcgaaacgtt gctctcttac cgaatgccgc aggtgttgca
    11401 gcgttttcgg caacgcgcac caaaagtgcg tttatcgttg cagtcgctga actgttacgt
    11461 gatccgtgat gcgctactga atgatgaggc cgatgtcggc gtcttttatc gcgtagggaa
    11521 tgatgatgcc ctgaaccgac gagagttggg tgaacaatcg ctggtgctgg tggcttcacc
    11581 gcaaattgca gatgtcgatt ttactgagcc gggaagacat aacgcctgta gctttattat
    11641 caacgaaccg caatgtgtct ttcggcagat ctttgaaagt acattgcgcc agcggcggag
    11701 cacggtggaa aacaccattg agctgttaag cattgaaagt atcaaacgtt gtgtcgcggc
    11761 gaatatcggt gtcagttact tgccccgctt tgcggtggaa aaagagcttg agagcggaga
    11821 attaattgaa ttgccttttg gcgaacagtc gcagaccatt acggccatgt gcgcacacca
    11881 tgctggaaaa gcggttagcc cggcgatgca cacttttatt cagtgtgttg aagagagttt
    11941 tgtggctgga taaaaataat gtcgggatta aacccggcat ttaacttgtc gacaaagtgc
    12001 gctttattca tgccggatgc ggcgtgaacg ccttatccgg cctacaagat cgtgcaaatt
    12061 caatatattg catgagccac gtaggcctga taagcgtagc gcatcaggca gttttgcgtt
    12121 tgccagcact tttaatacca ggcttaaacc tggcattgag attactcttc tgtcgtttgc
    12181 ggtggcacgc ccgttccgct tggtttacgg cgcttaccaa tatttttggt gtcgcgatgg
    12241 cgttttttca cccgcggctt ctctttctct ttagcttttt tcttctcagc acgtttagcc
    12301 agtactttct tcgatggctt gccggtctgc ttttcgcttg gcgcacgcgt tttcgggcgt
    12361 aactcatcaa taacgcgagc tttaattggc tcttcaatat agcggcctac tttacccagc
    12421 agcagatggt catgagcttc aaccagcgaa atcgctgttc ccttacgacc ggcgcgcgcg
    12481 gtacgtccga tacggtgcaa ataagtatca ccactgcgcg gcatatcgaa gttaaagacg
    12541 tggctgacgt caggaatgtc gataccgcgc gcggcaacat cggttgcaac cagtacgttt
    12601 acgcgacctt cggtcaaacg cttgatcgct tcgttacgct tgccttgtac catctcacct
    12661 tcgagatagc agttgttgat gcccgcttcg cgcagccagt ttgccagctc atgcacacgt
    12721 tcacgcttac gcacaaacac aattgagcgg gtcgcttccg gctgttttaa cagatgcacc
    12781 agcaacgcgg ttttatgctc aagatcatcg gcgcggtaat accactgatg aatttttttg
    12841 cgctcacggg tggacggatt ggcagaaact tccaccgggt cttccagcag acgctcggca
    12901 aagtcctgaa tcgcatcgcc ttccagcgtt gccgaaaaga gcagggtctg tttacgccag
    12961 cgcgtttcgc cagcaatatg ttcgatatcc tgagcgaagc ccatatccag catacggtca
    13021 gcttcgtcga ggatcagcgt ttcaactgcg cggcaatcga agttctcttc ttttatgtat
    13081 tgcagcagac gcccggtcgt ggcgaccacg atgtcctggt tttcgctgaa cacttccgcg
    13141 tggttcatat aggctacgcc gccggtgatg gtggcgatat ccagatgcgt atgtttcgcc
    13201 agttcgcggg catgatcggc cacctgcatc gccagctcgc gagttggggt gaggatcaaa
    13261 atacgcggcg gaccggattt cttacgtggg aaatcgagca ggtgctgcaa cgctggcagc
    13321 agatacgccg ccgttttacc ggtgcctgtc ggcgcagaac cgagtacatc acggccatcg
    13381 agcgcaggcg gaatggcggc agcctgaatg gcggtcgggc gagtgaaacc tttatcctgg
    13441 agggcttcca gcaggctttc gtcgagttca agttcggaaa aagtcgttac agtcatgttc
    13501 tacctctgtg tggggcgctg attatagacg ttacggctgc aatcttcatc tgtttgtatg
    13561 gatatcgctt ctcgggtatt gttctcaccc ggtgctatcg ccgtttttcc tgcaaggttt
    13621 tattttcatg tcacagtcta catccgtgct tcgtcgtaat ggatttactt ttaaacagtt
    13681 ttttgttgct cacgatcgtt gtgcgatgaa agtgggaacg gatggcattt tgctgggcgc
    13741 atgggcaccg gtggctgggg tgaaacgttg ccttgatatc ggcgcgggta gcgggttgct
    13801 ggcattaatg ctggcgcagc gaaccgatga cagcgtgatg attgatgcag ttgaactgga
    13861 aagtgaagct gccgcgcagg cgcaggaaaa tatcaaccag tccccgtggg cagagagaat
    13921 taacgtccat acggcggata ttcagcagtg ggtaacacag cagaccgcgc gctttgattt
    13981 aatcatcagc aacccgccgt attatgagca aggagtggag tgcgcgacgc cgcagcggga
    14041 acaggcgcgc tacaccacct cacttgatca ccagacttta cttacttgcg cggcggagtg
    14101 cattaccgaa gagggatttt tctgcgtggt gctgccagag cagattggta aaagttttac
    14161 ggagctggca ttaagtatgg gctggcattt acgtttgcgg acggatgtgg cggaaaacga
    14221 agcgcgactg ccgcatcggg tgttgctggc attctccccg caggcgggag aatgctttag
    14281 cgatcgctta gtcattcgtg ggccagacca gaactattcc gaagcgtata cggcgctgac
    14341 ccaggctttt tatctgttta tgtaatgatt gccgggagaa aggatcgatg gaccggaatg
    14401 cgtgagcagt tccgggtaat ccagcgtaaa atgcagtcca cggctctcct tgcgtatcat
    14461 cgcacagcga acaatcaact cggcaacctg taccagatta cgcagctcca gcaaattatt
    14521 tgagacgcgg aaatgggcgt aatattcgtc tatttcttgt tggagcatgg ttatccgccg
    14581 cagggcgcgc tcaaggcgct tcgttgtgcg cacaatgcca acgtaatccc acataaacag
    14641 ccgtagctcg tgccagttat gctgaattac tacccgttcg tcagggttct caacgcggct
    14701 ttcatcccac ggcggtaacg tactgacgcc gtgggcataa ggcatacgtc tggtgatatc
    14761 ttctgccgcc gaccagccat agaccagaca ctccagcaat gaattcgagg ccatgcggtt
    14821 agcgccgtgt aagccggtat aactcacctc accaatggca tacaaaccct cgacgtccgt
    14881 acgcccatga tcatcaacca ttacaccacc gcaggtataa tgtgcagcag gcacaatcgg
    14941 taccggttct tgtgtgagat caatccccag cccgagcaat ttttcataaa tcatcgggaa
    15001 atgctggcga ataaaatcgg cgggcttatg gctgatgtcg aggaacatac aatctgcgcc
    15061 gaggcgtttc atttcatggt caatggcgcg ggcgacaata tcgcgcgggg ccagttcgcc
    15121 gcgctcatca aaatcgggca taaaacgcgt gccatccggg cgcttgagat aagcgccttc
    15181 gccgcgcagt gcttccgtta acaggaaatt gcgtgcctgt gggtgataca gcgcggtagg
    15241 gtggaactga ttaaattcga gattggcaac ccggcagcct gcgcgccacg ccatagcaat
    15301 gccatcgcca gaagaaatat ccggattggt ggtgtactga taaaccttcg acgcaccgcc
    15361 ggttgccagc accaccgctt ttgcgtggca ggtttccacc gtttctttat ttcggttcca
    15421 tacccacgcg ccaacaaccc gtcgcgtgcc cggcaggcca attttgtcag aaataatcag
    15481 atcaaccgcg ttgctgcgct ccagcacgcg aatattcgga tggttcagcg ccttgctcac
    15541 cagcgtggtt tctacttctc taccggtggc gtcggcggca tgaagaatac gacggtgact
    15601 atgtccacct tcacgggtca gatggtagct ttcttcgcca ttcggctgaa tgtgggtatc
    15661 aaataacacc ccctggtcga ttagccattg cacacaggag cgtgcattgc tggcgacaaa
    15721 ttcaactgca tggcgatcgc aaataccagc cccggcaatc aatgtgtctt ccacatgcga
    15781 gtcaatgctg tcagtttcat caaacacggc ggcaataccg ccctgggcat aaaatgttga
    15841 accttccgtt accgggcctt tacttagaac gatgacctga tgctggtcag ccatgcgtaa
    15901 cgccagtgaa agcccggctg cgccgctacc gataatcaac acgtcacatg aatgttcagg
    15961 gagagtattc attttctttg tttaatttac taaacacggt ttggtcagca tagcatcatg
    16021 ttgtgcgaat aaacacctgc tattttaata tttgttacag tggctaaaca cgctgacgca
    16081 gggcggcgag aaaaagagaa gttactggct ggtgaaggat taggtggtga aataaaaagg
    16141 ccgttgggtt actcttcagg cagttaaatg ggcatttcta cacagataat gcgatgttca
    16201 gattctgtag acttataatg atagataatg atccgtctac agcatgacaa acaaaaacag
    16261 atgcgttacg gaactttaca aaaacgagac actctaaccc tttgcttgct caaattgcag
    16321 ctaatggagt ggcgtttcga tagcgcgtgg aaatttggtt tggggagact ttacctcgga
    16381 tgagcgagca gttaacggac caggtcctgg ttgaacgggt ccagaaggga gatcagaaag
    16441 cctttaactt actggtagtg cgctatcagc ataaagtggc gagtctggtt tcccgctatg
    16501 tgccgtcggg tgatgttccc gatgtggtac aagaagcttt tattaaagcc tatcgtgcgc
    16561 tggattcgtt ccggggagat agcgcttttt atacatggct gtatcggatt gctgtaaata
    16621 cagcgaaaaa ttacctggtt gctcaggggc gtcgtccacc ttccagtgat gtggatgcca
    16681 ttgaagctga aaacttcgaa agtggcggcg cgttgaaaga aatttcgaac cctgagaact
    16741 taatgttgtc agaagaactg agacagatag ttttccgaac tattgagtcc ctcccggaag
    16801 atttgcgcat ggcaataacc ttgcgggagc tggatggcct gagctatgaa gagatagccg
    16861 ctatcatgga ttgtccggta ggtacggtgc gttcacgtat cttccgagcg agggaagcta
    16921 ttgataacaa agttcaaccg cttatcaggc gttgacgata gcgggatact ggataagggt
    16981 attaggcatg cagaaagaac aactttccgc tttaatggat ggcgaaacgc tggatagtga
    17041 gctgcttaac gaactggctc ataacctaga aatgcagaaa acctgggaaa gctatcactt
    17101 aatccgtgat tcaatgcggg gtgatactcc cgaggtgctc catttcgata tctcttcacg
    17161 cgtgatggcc gccattgaag aagagccagt acgtcaaccg gcgacattga tcccggaagc
    17221 ccagcctgcg ccgcatcaat ggcagaaaat gccattctgg cagaaagtac gtccgtgggc
    17281 ggcacagctt acccaaatgg gcgtagccgc atgcgtatcg cttgcagtta tcgttggcgt
    17341 ccagcactat aatgggcaat ctgaaacgtc ccagcagccc gaaacgccgg tatttaatac
    17401 actgccgatg atgggtaaag ccagcccggt aagcctggga gtaccttctg aagcgaccgc
    17461 aaacaatggt caacagcagc aggtacagga gcagcgtcgt cgcattaatg caatgttgca
    17521 ggattacgaa ctgcaacgcc gactccactc tgaacagctt cagtttgagc aggcgcaaac
    17581 ccagcaagcc gctgtacagg tgccaggaat tcaaacttta ggaacgcaat cgcagtaatg
    17641 aagcaacttt ggtttgccat gtcattagtg acaggtagcc tgttattctc tgctaacgcc
    17701 tcggccactc ccgcgtccgg ggcgttgtta cagcagatga acctggccag tcagtcactg
    17761 aattacgagc tgtcattcat cagcatcaat aaacagggtg ttgagtctct gcgttatcga
    17821 catgcacgcc tcgataaccg tcctcttgca caattgttgc aaatggatgg cccgcgccgg
    17881 gaagtggtac agcgcggcaa tgaaatcagc tattttgaac cgggacttga accgttcacg
    17941 cttaatggcg attacattgt tgattctctg ccatcgctca tctataccga tttcaaacgc
    18001 ctttctcctt actacgactt tatctccgtc gggcgcacgc gtattgctga tcgtctttgc
    18061 gaagtcattc gcgtggttgc ccgagatggt acacgctaca gctacatcgt gtggatggac
    18121 accgaatcga aattaccgat gagggttgat ctccttgatc gcgatggtga aacgctggaa
    18181 caatttcgcg tgattgcttt taacgtcaat caggatatca gcagcagtat gcagacgctg
    18241 gcgaaggcaa atttgccgcc gttgctttct gttcctgtag gtgaaaaagc taaattcagc
    18301 tggacgccaa cctggttgcc acagggtttt agcgaagttt ccagtagtcg acgtccgcta
    18361 ccgacgatgg acaacatgcc tatcgaatca cgtctctatt ccgacggatt attcagcttc
    18421 tcggtaaacg ttaaccgcgc tacgccatcg agcaccgatc agatgttgcg caccggacgc
    18481 agaaccgtca gtacaagcgt acgtgataac gccgaaatca ccattgtcgg tgaactgccg
    18541 ccgcaaacgg cgaaacgcat tgccgagaat attaagttcg ggacagcgca atgatcaaag
    18601 agtgggctac cgtcgtctcc tggcaaaacg ggcaagcgct ggtcagttgt gatgttaaag
    18661 cctcatgcag cagctgcgct tcacgcgccg gttgcggtag ccgcgtgttg aataaacttg
    18721 gcccgcaaac cacgcatacc attgtcgtac cctgtgatga accgttagtg ccggggcaaa
    18781 aagtggaatt ggggatcgcc gaaggcagcc tgcttagctc cgcattactg gtttatatgt
    18841 cgccgctggt gggattattc ctcatcgctt cgctatttca gctactcttt gcttctgacg
    18901 tcgcagcatt atgtggtgcg gtcctcggcg gtattggcgg gttcctgatt gcgcgtggct
    18961 actcgcgtaa gtttgctgcc cgggcagaat ggcaaccgat catcttaagc gtggcattgc
    19021 cgcctggcct tgtgcgattt gaaacatctt cggaagacgc gagccagtga tttcactggc
    19081 tttgttgcat tttgcataac aaatgcgagg acgtttccag ataccacaat ccatacggta
    19141 tgaacatttc ctcgcctcaa tgttgtagtg tagaatgcgg cgtttctatt aatacagacg
    19201 ttaagctcag aacagcgact tctaaaagcc tggttaaccg ggcattaagg cacaataatc
    19261 atactttatg aagaatatac gtaacttttc gatcatagct cacattgacc acggtaaatc
    19321 gacgctgtct gaccgtatta tccagatctg cggtggcctg tctgaccgtg aaatggaggc
    19381 gcaggttctc gattccatgg atcttgagcg tgagcgtggc attaccatca aagcgcaaag
    19441 cgtgacgctg gactacaaag cgtctgacgg cgaaacctat cagcttaact ttatcgacac
    19501 cccgggccac gtagacttct cctatgaagt ttcccgttcg ctggctgcct gtgaaggtgc
    19561 attgctggtg gtcgacgccg ggcagggcgt agaagcgcaa accctggcaa actgctacac
    19621 cgccatggaa atggatctcg aagttgtgcc ggtactgaac aagattgacc tgccggcagc
    19681 cgatcctgaa cgcgtggcgg aagaaattga agatatcgtc ggcatcgacg ccaccgacgc
    19741 ggtgcgctgt tcagcgaaaa ccggcgttgg tgtgcaggac gttctcgaac gtctggtgcg
    19801 cgacattccg ccgccggaag gcgatccgga aggcccgttg caggcactaa ttatcgactc
    19861 atggttcgac aactacctgg gcgttgtttc acttatccgt attaaaaacg gcaccctgcg
    19921 taagggcgac aaagtgaaag tcatgagtac cgggcagacc tataacgccg accgtctggg
    19981 catcttcacg ccgaaacagg ttgaccgcac tgaactgaaa tgtggcgaag taggctggct
    20041 cgtatgtgcg attaaagata tccacggcgc tccagtcggc gataccttaa cgctggcgcg
    20101 taatccggca gaaaaggcgc tgcctggctt taagaaagtc aaaccgcagg tatacgccgg
    20161 tctgttcccg gtaagttccg acgactatga agccttccgt gacgcgctgg gtaaactcag
    20221 cctgaacgat gcctcactgt tctatgagcc ggaaagctcc agcgcgctgg gctttggttt
    20281 ccgctgcggc ttcctcggcc tgctgcacat ggagatcatc caggaacgtc tggaacgtga
    20341 atacgatctg gatctgatca ccactgcgcc gaccgtagtg tatgaagttg aaaccatgtc
    20401 aagagaagtt atctacgtcg acagcccatc caagctgcct gcggtaaata acatctacga
    20461 actgcgcgag ccgattgcag agtgtcacat gctgctgccg caggcatatc tcggcaacgt
    20521 tattacgttg tgcgtagaaa aacgcggcgt gcagaccaat atggtttacc acggtaatca
    20581 ggtggcgctg acgtacgaga tcccgatggc ggaagtggtg ctcgacttct tcgatcgcct
    20641 gaaatctacc tcgcgtggtt atgcgtctct ggattacaac ttcaagcgct tccaggcgtc
    20701 cgacatggta cgtgtagacg tattaatcaa cggtgaacgt gttgatgcgc tggcgttgat
    20761 cacccaccgt gataattcgc aaaaccgcgg tcgcgagttg gtggagaaga tgaaagatct
    20821 gatcccacgc cagcagtttg atatcgccat tcaggcagcg attggtacgc acatcattgc
    20881 gcgatccacc gtgaaacagc tgcgtaaaaa cgtactggct aaatgttatg gcggcgatat
    20941 cagccgtaag aaaaagctgc tgcagaagca gaaagaaggt aagaaacgca tgaagcagat
    21001 cggtaacgtc gagctgccgc aggaagcgtt cctcgccatt ctgcacgtcg gcaaagacaa
    21061 caaataaccc ttaggagttg gcatggcgaa tatgtttgcc ctgattctgg tgattgccac
    21121 actggtgacg ggcattttat ggtgcgtgga taaattcttt ttcgcaccta aacggcggga
    21181 acgtcaggca gcggcgcagg cggctgccgg ggactcactg gataaagcaa cgttgaaaaa
    21241 ggttgcgccg aagcctggct ggctggaaac cggtgcttct gtttttccgg tactggctat
    21301 cgtattgatt gtgcgttcgt ttatttatga accgttccag atcccgtcag gttcgatgat
    21361 gccgactctg ttaattggtg attttattct ggtagagaag tttgcttatg gcattaaaga
    21421 tcctatctac cagaaaacgc tgatcgaaac cggtcatccg aaacgcggcg atatcgtggt
    21481 ctttaaatat ccggaagatc caaagcttga ttacatcaag cgcgcggtgg gtttaccggg
    21541 cgataaagtc acttacgatc cggtctcaaa agagctgacg attcaaccgg gatgcagttc
    21601 cggccaggcg tgtgaaaacg cgctgccggt cacctactca aacgtggaac cgagcgattt
    21661 cgttcagacc ttctcacgcc gtaatggtgg ggaagcgacc agcggattct ttgaagtgcc
    21721 gaaaaacgaa accaaagaaa atggaattcg tctttccgag cgtaaagaga cactgggtga
    21781 tgtgacgcac cgcattctga cagtgccgat tgcgcaggat caggtgggga tgtattacca
    21841 gcagccaggg caacaactgg caacctggat tgttcctccg ggacaatact tcatgatggg
    21901 cgacaaccgc gacaacagcg cggacagccg ttactggggc tttgtgccgg aagcgaatct
    21961 ggtcggtcgg gcaacggcta tctggatgag cttcgataag caagaaggcg aatggccgac
    22021 tggtctgcgc ttaagtcgca ttggcggcat ccattaatag ccatcttcgt tcacgttgtc
    22081 gccgttatgg cgacaacgtg aattatttat gagataaatc tcccgtggct aacgacatcc
    22141 cccgtcgttg tgtatagaat attcccccga agtttaaggt tggcacctcc aggttgccac
    22201 ggcacacgaa acagcgttgg tccccatata ccggtaaact gaaactgcag cgaagcagtt
    22261 agcagaacca tgtatatcag gtctgtttcg tgtgctgaat tgttgacgca tttatttatt
    22321 ggtatcgcat gaaccccatc gtaattaatc ggcttcaacg gaagctgggc tacactttta
    22381 atcatcagga actgttgcag caggcattaa ctcatcgtag tgccagcagt aaacataacg
    22441 agcgtttaga atttttaggc gactctattc tgagctacgt tatcgccaat gcgctttatc
    22501 accgtttccc tcgtgtggat gaaggcgata tgagccggat gcgcgccacg ctggtccgtg
    22561 gcaatacgct ggcggaactg gcgcgcgaat ttgagttagg cgagtgctta cgtttagggc
    22621 caggtgaact taaaagcggt ggatttcgtc gtgagtcaat tctcgccgac accgtcgaag
    22681 cattaattgg tggcgtattc ctcgacagtg atattcaaac cgtcgagaaa ttaatcctca
    22741 actggtatca aactcgtttg gacgaaatta gcccaggcga taaacaaaaa gatccgaaaa
    22801 cgcgcttgca agaatatttg cagggtcgcc atctgccgct gccgacttat ctggtagtcc
    22861 aggtacgtgg cgaagcgcac gatcaggaat ttactatcca ctgccaggtc agcggcctga
    22921 gtgaaccggt ggttggcaca ggttcaagcc gtcgtaaggc tgagcaggct gccgccgaac
    22981 aggcgttgaa aaaactggag ctggaatgag catcgataaa agttactgcg gatttattgc
    23041 catcgtcgga cgtccgaacg ttggcaaatc cacattgttg aacaaactgc tggggcagaa
    23101 aatctccatc acttcccgca aggcgcagac aactcgtcac cgcattgtgg ggatccatac
    23161 tgaaggcgcg tatcaggcga tctacgtcga tacaccgggc ctgcatatgg aagaaaaacg
    23221 cgccattaac cgcctgatga acaaagcggc gagcagctct attggcgatg ttgagctggt
    23281 gatttttgtc gttgaaggca cccgctggac gccggacgac gaaatggtgc tcaacaaact
    23341 gcgcgaaggc aaagcgccgg taatcctcgc ggtgaacaaa gtggacaacg tgcaggagaa
    23401 agccgatctg ctgccgcacc tgcagttcct ggcaagccag atgaacttcc tcgatatcgt
    23461 gccaatctct gccgaaaccg ggctgaatgt tgacactatt gcggcaatcg tgcgtaagca
    23521 tctacctgaa gcgactcatc acttcccgga agattacatc accgatcgct cacagcgttt
    23581 tatggcgtct gaaatcatcc gcgaaaaact gatgcgtttc ctcggcgctg aactgccgta
    23641 ctccgtgacc gtggagatcg aacgtttcgt ctctaacgaa cgcggtggtt atgacatcaa
    23701 cggtttgatt ctcgttgagc gtgaagggca gaagaagatg gttattggca acaaaggggc
    23761 caagatcaaa accatcggga ttgaagcgcg taaagacatg caggaaatgt tcgaagcgcc
    23821 tgttcacctt gagctgtggg taaaagtgaa atccggttgg gccgacgacg aacgcgcact
    23881 gcgcagtctc ggttacgttg acgatcttta agagttactc cgatggaagg ctggcagcgc
    23941 gcatttgtcc tgcatagtcg cccgtggagc gaaaccagcc tgatgctgga cgtcttcacg
    24001 gaggaatcgg gtcgcgtgcg tctggttgcc aaaggcgcac gctctaaacg ctctactctg
    24061 aaaggtgcat tacagccttt cacccctctc ttgctacgtt tcggcgggcg tggcgaagtc
    24121 aaaacgctgc gcagtgctga agctgtctcg ctggcgctgc cattaagcgg tatcacgctt
    24181 tacagcggtc tgtacatcaa cgaacttctc tcccgcgtac tggaatacga gacgcgcttc
    24241 tctgaactct ttttcgatta cttgcactgc attgagtctc ttgcaggtgc cactggtacg
    24301 ccagaacccg cgctgcgccg ctttgaactg gcactgctcg ggcatctggg ctatggcgtc
    24361 aattttaccc attgcgcggg tagcggcgag ccggtagatg acaccatgac gtatcgttat
    24421 cgcgaagaaa aagggtttat cgcaagcgtc gttatcgaca ataaaacgtt caccggaagg
    24481 cagttaaaag cgttaaacgc gcgggaattt cctgacgcag acacactgcg cgccgcgaaa
    24541 cgctttaccc gcatggcgct taagccgtat cttggcggta aaccattaaa gagcagggaa
    24601 ctgttccggc agtttatgcc taagcgaacg gtgaaaacac attatgaatg atgaggattg
    24661 tcatggctga attactgtta ggcgtcaaca ttgaccatat tgcaacgctg cgtaacgcac
    24721 gcggtactgc ttacccggat ccggtgcagg cggcgtttat tgccgagcag gcgggagcgg
    24781 acggcattac cgtgcattta cgtgaagatc gccgtcacat tactgaccgc gacgtgcgca
    24841 tcctgcgtca gacgctggat acccgtatga atctggagat ggcagtgacc gaagagatgc
    24901 tggcgatcgc cgttgagacg aagccacatt tttgctgcct ggtaccggaa aagcgtcagg
    24961 aagtaacaac cgaaggcggc ctggacgttg cagggcagcg tgaaaaaatg cgcgatgcct
    25021 gcaaacgtct ggcagatgcc gggatccagg tttctctctt tatcgacgcc gatgaagagc
    25081 agatcaaagc tgcggcagag gttggcgcac cgtttatcga gatccacacc ggttgctatg
    25141 ctgatgccaa aactgacgcc gaacaggcgc aagagctggc gcgtatcgcc aaagccgcga
    25201 cctttgccgc tagtctcggt ctgaaagtta acgctggtca cggtctgacc tatcacaacg
    25261 tgaaagctat tgccgccatc cctgagatgc atgaactgaa tatcggtcat gccattattg
    25321 gtcgtgcagt gatgaccgga ctgaaagatg cggtggcaga aatgaagcgt ctgatgctgg
    25381 aagcgcgtgg ctaatggcaa tattaggttt aggcacggat atcgtagaga tcgctcgcat
    25441 agaagcagtg atcgcccgat ccggtgaacg cctggcacgc cgcgtattaa gcgataacga
    25501 atgggcaatc tggaaaacgc accaccagcc ggtgcgtttt ctggcgaagc gttttgctgt
    25561 gaaagaagcc gcagcaaaag cgtttggcac cgggatccgc aatggtctgg cgtttaatca
    25621 atttgaagta ttcaatgatg agctcggcaa accacggcta cggctatggg gtgaggcatt
    25681 aaaactggcg gaaaagctcg gtgttgtaaa tatgcatgta acgctggcag atgagcggca
    25741 ctatgcttgt gccacggtaa ttattgaaag ttaaaaataa taccgttctc tttaaaaagt
    25801 aaaacccccg aactggtaag gaacgggggt tttacttttt aactttaatt ttgtgcagca
    25861 tgcaccctca acaacagcga ttatggatca gtgtacttat cttttacaag caacttgcaa
    25921 tctttagcat aaaaactcga gcctttacga agaaagcaat attgatggaa agattaacgt
    25981 gaccgccaat tcgtaagtac attaaaattg gcttcgttat tgaagatttt gctgtgcttt
    26041 acaccatgcc acagaattcc cccattgaaa cgagtggtgt cgtcaaaact ctggtgtgga
    26101 gtgcagcatg caccctcaat aactcgcacg ttcagttttg gggagatgta agggctaatc
    26161 tgaatggctg cattccttgt ttaaggaaaa acgaatgact gattgccgat acctgattaa
    26221 acgggtcatc aaaatcatca ttgctgtttt acagctgatc cttctgttct tataacacaa
    26281 ggaaacgtac ttaaggtgcg tccggtgaac cagtcggacg cacctttaat aactataaat
    26341 aagtgtctgg gcagatacta tataaattaa cttagtgaat gattatgcta atgtcatcaa
    26401 ttaaataaat ataatggcgt taaggcttcc cagtaatata attaatactc tacttccaga
    26461 gtagaatatt aaattttatc cgcgtggtgc atcagcacaa atttatccca caactgttct
    26521 tctgtctcga catgcgccgg atctttcaca atagtattgg ggatcgggca caccttctgg
    26581 caggttggtg tctcgtagtg ccctacgcat tcggtacact tatcgctgtt aatctcgtag
    26641 atatgatctc ccattgaaat cgcctcattc gggcattcgg gttcacacat atcacaattg
    26701 atgcattttt tagtgattaa caacgccata aggaaactct caaaacaaca caaaccgggc
    26761 gggcattata cgcgcattct ttactcagac cagttttttc accagcgctt cactatgacg
    26821 aatacgttct ggtgccagtt ccagatcctg ctgaatcagc gcaatgaaca gcaaatccgt
    26881 taacattccc tgagcgtgac aagcagagat tgaagcactg tttgtcgctt gttcttcggc
    26941 aatggtatac aggcaatgag aagcacgctg ttgcagggca ttcggagtaa agccggtaat
    27001 cgccagcact tttccgccca ctcgcagcat ctcatctgtc gccaggttta actcgcgtcg
    27061 tacaccggtg taggaaatgg ctaataacag atcgtcaggg gacgacgcct gtactgttgc
    27121 gagtagcgca tgcatatcgc gcactgcggc agcattgaag ccaatcttca tcagcttcca
    27181 ggcaaagttt tgcgccacca gacccgaagc gccaataccg gtcagaatta tccgccgcgc
    27241 agagcgcaac attgttacgc attcatgcag tttctcttca ctattaacgt ttagcgttgc
    27301 gtacatagcg gcagtatttt ctttgatcag tttttcgccg accagccgta acggatcatc
    27361 accgcggatt tggttatgaa tgggcacgga gggtgattcc ggctggcttg ccagcgcttc
    27421 actcaacgcc aacttaagcg ccggaaaacc tttatagccg agtttttgcg cgaacttcac
    27481 gacgctggac tgactgactc cggcttcgtt ggccagttgc tgagagctta aatggcgcgc
    27541 cgtatcaggt tgtagcagca gataatccgc cagtttttta tcactctggg caagcccctg
    27601 gtaacgctga cggatacgaa gtaagccgtt catatctttc ctcgcgatga aatagtggcg
    27661 acaacaggca atatgagaca cacgcgagtg aattttatat tccattgccg ctaattgcca
    27721 tgaattaaaa attccagtta gccatgatgc ggtcgattgt tgccggaaaa gtgatgatct
    27781 atctgttagg ctatgtagta gcgaaaatta atcaggatgt ttcagtccag aggagtatgg
    27841 tttggcaact cacgagcgtc gtgtggtgtt ttttgactta gatggaacat tgcatcagca
    27901 ggatatgttc ggcagttttc tgcgctattt actacgtcgc caaccgctga atgcgttact
    27961 tgtcctgccg ttgttaccga ttatagccat tgcgttattg ataaaaggtc gtgcggcacg
    28021 caggccgatg agtctgcttc tgtgggggtg cacttttggt cacagcgaag cacgtttaca
    28081 gacgttgcag gccgatttcg tgcgctggtt tcgcgacaat gttaccgcct ttccgctggt
    28141 tcaggagcga ttaaccacct acctgttaag ttccgatgct gatatctggt tgattaccgg
    28201 ctctccgcag ccgctggttg aagcggttta tttcgatacg ccctggctgc cgcgggttaa
    28261 tcttatcgcc agccaaattc agcgtggcta tggtggttgg gtattgacga tgcgttgtct
    28321 gggacatgaa aaggtcgcac aactggagcg caaaatcggc actccgctgc ggctgtacag
    28381 tggctatagc gacagtaatc aggacaatcc gctgctttat ttctgtcagc atcgttggcg
    28441 agtaaccccg cgcggtgaac tccagcaact ggaatagagt aaagcatagc gtccgtgtat
    28501 aatgcgccgc gcttttataa ccggagtttt ctttttgtct gaagtcgaat ttagccacga
    28561 atactggatg cgtcacgcgc tgacgctggc gaaacgtgcc tgggatgagc gggaagtgcc
    28621 ggtcggcgcg gtattagtgc ataacaatcg ggtaatcggc gaaggctgga accgcccgat
    28681 tggtcgccat gatcccaccg cacatgcaga aatcatggcc ctgcggcagg gtggtctggt
    28741 gatgcaaaat tatcgtctga tcgacgccac gttgtatgtc acgcttgaac catgtgtaat
    28801 gtgtgccgga gcgatgatcc acagtcgcat tggtcgcgtg gtctttggtg cgcgtgacgc
    28861 gaaaactggc gctgcgggat ctttaatgga tgtgctgcat catccgggta tgaatcaccg
    28921 agtggaaatt acggaaggaa tactggcgga tgagtgcgcg gcgttgctca gtgacttctt
    28981 tcgcatgcgc cgccaggaaa ttaaagcgca gaaaaaagcg caatcctcga cggattaatt
    29041 ttgtttctct tcactccctt tcctggaaaa cagcagagag ggagaatggg tcaaatagtt
    29101 tgatgacgac tgggaaagaa acgagagaaa aggaaatttc tctttgccca actccgtagg
    29161 cgataccgcc ggataatcct gcgccagttg catcgccgct tctgtagcct gcttctcttt
    29221 ctcttgcaga taacccacca ggctaatctg atacttacga atattttcga cataagcgta
    29281 ggcttcatgt ccacgagcgt agccgtaagt cagcttgctg taatagggtt tctggctaag
    29341 taaaggcaga cgctgtttta cgtcagccca actgtcagga ttccctttgg tttttgccgt
    29401 cagggcgcgg gcatccagca tatgcgcata gcccatattg tacgcagcga gggcaaacca
    29461 gatccgctcg ttctccggca cactttccgg cactttactc atcatatcct gcaaataacg
    29521 cacgccaccg ctgatgctct gttcggcatc ggtacgatcc gtaatgccga ggctttgcgc
    29581 ggtatttttg gttaacatca tcatgccgcg cacacccgtc ggtgaagtgg cctgtgcatc
    29641 ccagtgcgat tcctgataag caatagcggc cagcaaacgc cagtcaattt cttcggcgta
    29701 tttctcaaac aggggcttta actgcggcag taccgcatcg acggcgcgta aaaatgtgcg
    29761 cgtatcgacg taatcaaaat catcgccatg ccccaggtat ttctcttcaa tgcgtgccag
    29821 cgtaccgtct tcattcattt cgttgaagaa gtcgagcagg gcggcggaaa gggtattatc
    29881 gccatctaac gggctaaacc aagtcaccgg ttgttcatcg gtgatatcga gcgctacggc
    29941 gagctccggg tgaacgcgct gaaacaggct gatggcgaca gaatcagcaa tggtgtaatc
    30001 gagttttcct tcgatgacat cttccattaa ttctgcagag ccttttttgt cgtctacctt
    30061 ccagcttaat tccgggaatt ttgtttcttt cagggtctgg agatcgttaa ccaccacatg
    30121 acccggtgca acggtgagtt gctccgccgt caggttgccc agcgtacgtg ggcgatactg
    30181 acccacttta taaaccagtt gttgtgacac ggaataatag gtagggccag gctgataatt
    30241 ttttacccgc tcactgttat agacaagtcc tgccgccagc aggtcggcgt taccattatc
    30301 aaggtcgtca aacagctggc tgatattctg ccgcacggtc actttcagtt ttacgccgag
    30361 gtaatcggca aactgtttcg ccagttcgta atccaggcca aaaggtttcc cgttgatttc
    30421 gttataagtc aggggagtat gaatggtgct cacacgcaac tctccccgcg cttgaatggc
    30481 ggcgatacgg ttgtcggctt taccaaacca gggaatggat ggccagagag cgaccgcgag
    30541 cagcagtgcc agaatgccga tgaacagata attaatcttt aattttttca attagttaat
    30601 tctctgtgtc gtgcgcgtcc cagcttgaaa aaacgtaata atagtgaaag gtttactcat
    30661 aaatgagcgg cattttgcgt aaacctgcgc cagatggcaa cttattacag ccattggcgg
    30721 cacgcgttgc taattcacga tggtgatttt atttccacgc aaacggtttc gtcagcgcat
    30781 cagattcttt ataatgacgc ccgtttcccc cccttgggta caccgaaagc ttagaagacg
    30841 agagacttat gatggaaatt ctgcgtggtt cgcctgcact gtcggcattc cgaatcaaca
    30901 aactgctggc acgttttcag gctgccaggc tcccggttca caatatttac gccgagtatg
    30961 tccattttgc tgacctcaat gcgccgttaa acgatgatga gcacgcacaa cttgaacgcc
    31021 tgctgaaata tggcccggca ctcgccagcc acgccccgca aggcaaactc ctgctggtga
    31081 ccccgcgtcc tggcaccatc tctccctggt cttcgaaagc gaccgatatt gcccataact
    31141 gcgggctaca acaggtaaac cgccttgagc gcggcgttgc ttactatata gaagccggta
    31201 cgctgaccaa tgaacaatgg cagcaggtta ccgctgaact gcacgaccgc atgatggaaa
    31261 cggtcttttt tgctttagat gatgcagagc agttgtttgc ccaccatcaa ccgactccgg
    31321 ttaccagcgt tgatttgctg gggcagggcc gtcaggcgct gatcgacgct aacctgcgtc
    31381 ttggcttggc tctggcggaa gatgaaattg actatctgca ggatgctttc acaaagcttg
    31441 gtcgtaaccc gaacgacatc gaactgtata tgtttgccca ggcgaactcc gagcactgcc
    31501 gccacaaaat ttttaacgcc gactgggtta tcgatggtga acagcagccg aaatcgctgt
    31561 tcaagatgat caaaaatact ttcgaaacca cgccagatca cgttctctct gcttataaag
    31621 ataacgccgc cgtaatggaa ggttctgaag tgggccgcta ctttgctgac cacgaaacgg
    31681 gccgctacga tttccatcag gaaccggcgc atattctgat gaaagtcgaa actcacaacc
    31741 acccgacggc gatttctccg tggccgggcg cggcgaccgg ttccggcggt gaaatccgcg
    31801 atgaaggtgc caccgggcgc ggcgcaaagc cgaaagcggg tctggttggt ttctccgttt
    31861 ccaacctgcg aattcctggc ttcgaacagc cgtgggaaga agatttcggt aagcctgagc
    31921 gcattgtcac cgcgctggac atcatgaccg aaggcccgct gggcggcgcg gcgtttaaca
    31981 acgaatttgg tcgtccggca ctgaacggct acttccgtac ttatgaagaa aaagtgaaca
    32041 gccacaacgg cgaagagctg cgcggttatc acaaaccgat catgctggcg ggcgggatcg
    32101 gcaacattcg cgccgatcac gtacaaaaag gcgagatcaa cgtcggtgcg aagttggtcg
    32161 ttctcggcgg cccggcaatg aacatcggtc ttggcggtgg tgcagcgtct tctatggcgt
    32221 ctggtcagtc tgatgccgac ctcgactttg cttccgtaca gcgcgacaac ccggagatgg
    32281 agcgtcgctg ccaggaagtg atcgaccgtt gctggcagct tggtgatgcc aacccaatcc
    32341 tgtttatcca cgacgttggc gctggcggtc tttctaacgc catgccggaa ctggtgagcg
    32401 acggcgggcg cggcggtaaa tttgaactgc gcgagattct aagcgacgaa ccgggcatga
    32461 gcccgctgga aatctggtgt aacgaatccc aggaacgcta cgtgctggcg gttgctgccg
    32521 atcaattacc gctgtttgac gaactgtgta agcgtgagcg cgcaccctac gcggtgattg
    32581 gtgaagcgac cgaagaactg catctttctc tgcacgatcg tcattttgat aatcagccga
    32641 tcgatctgcc gctggacgtc ctgcttggta aaacgccgaa gatgacccgc gatgtacaaa
    32701 cgctgaaagc gaaaggcgac gcgctggccc gtgaagggat caccattgca gacgcggtga
    32761 aacgtgtgct gcatctgccg actgtggcgg aaaaaacctt cctggtgacc attggcgacc
    32821 gcagcgtaac cggcatggta gcgcgcgatc agatggtggg gccgtggcag gtgccggtcg
    32881 ctaactgcgc ggtcactacc gccagcctcg acagctacta cggtgaagcg atggcgattg
    32941 gcgagcgtgc gccggttgcg ctgctggatt tcgccgcctc tgcccgtctg gcggtcggtg
    33001 aagcgttaac caacatcgcc gcaacacaaa ttggcgatat caaacgcatc aaactttccg
    33061 ccaactggat ggcggcggca ggccaccctg gtgaagatgc gggcctgtat gaagccgtta
    33121 aagccgtggg cgaagagctt tgtccggcgc tgggcctgac gatcccggtg ggtaaagact
    33181 ctatgtcgat gaaaacccgc tggcaggaag gtaacgaaga gcgcgaaatg acgtcgccac
    33241 tgtcgctggt gatttctgca tttgcccgtg tggaagatgt gcgtcacacc atcacgccgc
    33301 agctttctac cgaagataac gcattactgc tgattgatct gggcaaaggc aataacgcgc
    33361 tgggcgcaac ggcgctggcg caggtttatc gtcagcttgg cgataaaccg gcagatgtac
    33421 gcgacgtcgc gcaactgaaa ggtttctatg acgcggttca ggcgctggta gcacagcgta
    33481 agctgctcgc gtaccacgac cgctctgatg gcggcctgct ggtaacgctg gcggaaatgg
    33541 cctttgctgg tcattgcggc attgacgcgg atatcgccac tctgggtgac gatcgcttgg
    33601 cggcgttgtt taacgaagaa ctgggtgcgg tgattcaggt tcgagccgct gaccgtaaag
    33661 cagttgaagc ggtactggca cagcatgggc ttgctgattg cgtccactat gttggccagg
    33721 cggtttccgg tgaccgtttt gtgattaccg ccaacgggca gactgtattc agcgaaagcc
    33781 gcaccacgtt gcgtgtctgg tgggcagaaa ctacctggca gatgcagcgc ctgcgtgaca
    33841 acccggagtg tgctgaccag gagcaccagg cgaaatcaaa cgacgccgat ccgggcctga
    33901 atgtaaaact gtcgttcgat atcaacgaag atgtggctgc accgtttatt gccactggcg
    33961 cgcgcccgaa agttgccgta ctgcgtgagc agggcgtgaa ctcgcatgtt gaaatggcgg
    34021 cagctttcca ccgtgcaggc ttcgatgcta tcgacgtgca tatgagcgat ctgctggctg
    34081 gacgcacggg actggaggat ttccacgccc tggtcgcgtg cggtggtttc tcctacggtg
    34141 atgtgctggg tgccggtgaa ggttgggcga agtctatcct gttcaatgac cgtgtacgcg
    34201 atgagtttgc aaccttcttc caccgtccgc aaacgctggc gctgggggta tgtaacggtt
    34261 gccagatgat gtctaatctg cgcgaactga tcccgggtag tgagttgtgg ccacgttttg
    34321 tgcgtaacac ctccgatcgc tttgaagcgc gtttcagcct ggttgaagta acccaaagcc
    34381 cgtctctgct gttgcagggg atggtgggct cgcaaatgcc gattgctgtc tctcatggtg
    34441 aagggcgcgt ggaagtgcgt gatgcgacgc atctggcggc actggaaagc aaagggctgg
    34501 tggcactgcg ctatgtcgat aacttcggca aagtcactga aacctacccg gctaacccga
    34561 acggttctcc gaatggtatt acggcggtca cgactgaaag tggtcgagtc accattatga
    34621 tgccgcaccc ggaacgtgtt ttccgtactg tcagcaactc ctggcatccg gaaaactggg
    34681 gcgaggatgg cccatggatg cgtattttcc gcaatgcgcg taagcagttg gggtaagtcg
    34741 tcagcccatt ggttttgcag cccggatgcg gcattaagca ccaaccacta tcataagctc
    34801 ccaactccgg gagctttttt gtgtctgtaa atcacgacaa tggatggttt gctgtgtcgc
    34861 tttttggtga cgtttaactc attgatttta atgtcatcta ataagtttat cttaagttgt
    34921 ctctttttag cgacacagtg gctgatttac attcaaacag cggaggagac ataaacccct
    34981 caaagcaacg tataatcagt acgttaccaa actattttct ttattggcac agttactgca
    35041 taatagtaac cagtggctca ttcaccgact tatgtcagcc ccttcgggac gtgctacata
    35101 aaatacgaat gacgcacaac aaggtgcctg ccgtccaact tctgatatca gcgtagctat
    35161 atcaaccatc gggcgaaacg tcgagttagg caccgcctta ttccataaca aagccgggta
    35221 attcccggct ttgttgtatc tgaacttccc ctcggttagc atcaggctat tcgcgtctga
    35281 cgagagtaac accttgaaac gctggcccgt ttttccccgc tcattacgac aactggtaat
    35341 gctggcattt ttgctgattc tgctgcccct gttggtgctg gcatggcaag cctggcaaag
    35401 cctgaatgcg cttagcgatc aggcggcgct ggttaaccgc actacgctta ttgatgcccg
    35461 gcgcagtgaa gcgatgacca acgcggcgct ggagatggag cgtagttacc gtcagtattg
    35521 cgtgctggac gacccaaccc tggcgaaggt ttatcaaagc cagcgcaagc gttacagcga
    35581 aatgctcgat gcccacgcag gcgtgctgcc ggacgataaa ctctaccagg cattacgtca
    35641 ggatttgcac aatctggctc aacttcagtg taacaacagc ggtcccgatg ctgccgccgc
    35701 cgcacgtctt gaagcctttg ccagtgccaa taccgaaatg gtacaggcca cgcgcacagt
    35761 ggtgttctct cgtgggcagc agcttcagcg tgaaatcgcc gaacgtgggc aatattttgg
    35821 ttggcaatcg ctggtgctat ttctggtgag tctggtaatg gtactgcttt tcacgcggat
    35881 gattatcggg ccggtgaaaa atatcgagcg catgatcaac cgtctggggg aagggcgttc
    35941 tctgggcaat agcgtctcgt tcagtggacc gagcgagtta cgctcggttg ggcaacgtat
    36001 tctttggtta agtgagcgcc tgtcatggct ggaatcccaa cgccatcaat ttttaagaca
    36061 tttatctcat gaattaaaaa cgccactggc gagtatgcgc gagggcactg aattactggc
    36121 tgaccaggtt gtcgggccgc ttacgccaga gcaaaaagag gtggtgagca ttcttgatag
    36181 cagcagccgc aatttgcaaa aactgatcga acaactgctt gattacaacc gtaaacaggc
    36241 ggacagtgcg gtggaactgg agaatgttga gttagcaccg ctggtggaga cagtggtttc
    36301 tgctcatagc ctgcccgcac gggctaaaat gatgcatacc gacgtcgatc tcaaagcaac
    36361 agcttgcctg gcggagccaa tgctgctgat gagcgtactg gataatcttt actccaatgc
    36421 ggtgcactac ggggctgaat ccggtaacat ttgccttcgc agcagtttac atggtgcgcg
    36481 ggtttatatt gatgtcatca atacaggcac gcccattccg caagaggaac gcgccatgat
    36541 cttcgaaccc ttttttcagg gaagccacca gcgaaaaggg gcggtgaagg gcagcggtct
    36601 gggattaagc attgccaggg attgtattcg ccgtatgcaa ggggaactgt atctggtcga
    36661 cgagagcggg caagacgttt gtttccgcat tgaattaccg tcgtcgaaaa acacgaaata
    36721 atgctaacag cctatcctat caggctattt tatttgtcag ttcggccccg ggcagtgctc
    36781 gaaatcttca cgtacgatat gtacgctccg gtttctccgc gctgtccatg tccagactgt
    36841 ctgcaacaat tacgcccact gggataggct ttaagtctgg tgaatatgcg acacattttt
    36901 caacgattat tgccccgacg gctgtggctg gcagggctgc catgtctggc attgctgggt
    36961 tgcgtgcaga atcacaataa gccagccatt gatacgccag cagaagaaaa aattccggtt
    37021 tatcaactgg ctgactatct ttctactgaa tgtagcgaca tctgggcgct gcaaggtaaa
    37081 tcaaccgaaa ccaatccgct ttactggctg cgggcgatgg attgtgctga tcgtttaatg
    37141 cctgcgcagt cgcgtcagca agctcgccaa tatgacgacg gcagctggca aaataccttt
    37201 aaacaaggga ttttgctcgc cgacgccaaa attacgccgt atgagcgccg ccagctggtc
    37261 gcgcgtattg aagcgttaag taccgagatc ccggcacagg ttcgcccact gtatcaactc
    37321 tggcgcgacg gtcaggcgct gcaactgcaa ctggcggaag agcgccaacg ttacagtaaa
    37381 ctccagcagt ccagcgacag cgagctggat acattgcgcc agcaacatca cgttctacag
    37441 cagcaactgg aactcaccac ccgcaagctg gaaaacctga ccgatattga acgccagctc
    37501 tcgacccgca aaccggctgg aaatttctcg ccggatacgc cgcacgagag tgaaaagccc
    37561 gcgccatcca cccatgaggt cactcctgat gagccataaa cctgcgcatt tattattggt
    37621 cgatgacgat ccgggattgc tgaaactgct tggcctgcgc ctgaccagcg aaggctacag
    37681 tgcggtcacg gcggaaagtg gcgctgaagg attacgggta ctgaatcgcg aaaaagtaga
    37741 tttagttatc agcgacctgc ggatggatga aatggacggt atgcagctgt ttgctgaaat
    37801 ccagaaagtg cagccgggaa tgccggtaat tattcttacc gcgcatggtt ctattcccga
    37861 tgccgttgct gcaacacagc agggcgtttt tagtttcctc accaagcctg tcgataaaga
    37921 tgcgctatat caggcaattg acgatgcgct ggagcaatcc gcgccagcca ccgatgaacg
    37981 ctggcgcgag gcaattgtca cccgcagccc gctgatgctg cgtttgctgg aacaggcgcg
    38041 gctggtggcg caatcagacg tcagcgtttt gattaatggc cagagcggca ccgggaaaga
    38101 gattttcgcc caggctatcc acaacgccag cccgcgcaat agcaaaccat ttattgctat
    38161 taactgtggc gcattgcccg agcaattgct ggagtcggag ctgtttggtc atgcgcgtgg
    38221 cgcgtttact ggcgctgtca gcaatcgcga aggtttattc caggcggcgg aaggcggtac
    38281 gctatttctc gatgaaattg gcgatatgcc tgcgccgtta caggtcaaac tgctgcgcgt
    38341 gttgcaggag cgtaaagtgc gcccgctggg cagtaaccgc gatattgata tcaatgtgcg
    38401 gattatttct gccactcacc gtgatctgcc aaaagcgatg gcgcgcgggg aattccgcga
    38461 agatctctat taccgcctca acgttgtcag cctgaaaatt ccggcactgg cggagcgcac
    38521 agaagacatt ccgctactgg caaatcacct gttgcgccag gcggcagagc gacataaacc
    38581 gtttgtccgc gcgttctcta ccgatgcgat gaaacgcctg atgaccgcga gctggccggg
    38641 taatgtgcgc cagttggtca acgtgattga acagtgcgtg gcgctgacct catctccggt
    38701 gattagtgat gcgctggtgg agcaggcgct ggagggtgaa aatacggcgc tgccaacctt
    38761 tgttgaggca cgtaatcagt ttgaactcaa ctatttgcgt aagctgctgc aaatcaccaa
    38821 aggcaacgtc acccacgcgg cgagaatggc ggggcgcaac cggacagaat tttataaact
    38881 gctttcccga cacgagctgg atgcaaacga tttcaaggaa tgaattggcg ttatgtgtta
    38941 cgtttagcag atcaaaagac aggcgacctt ttcaaggaat agcatgaaaa agattgatgc
    39001 gattataaaa cccttcaagc tggacgatgt ccgcgaagca ctggccgaag tcggtattac
    39061 cggcatgacg gtgaccgaag tgaaaggctt tggtcgccag aaaggccata ccgagctgta
    39121 ccgcggcgcg gagtatatgg tggattttct gccgaaagtg aaaattgaga ttgtcgtacc
    39181 ggacgacatt gtcgatacct gtgtcgatac cattattcgc acggcgcaaa ccggcaaaat
    39241 cggtgacggt aaaatcttcg tctttgacgt ggcacgggtc attcgcatcc gtaccggtga
    39301 agaggacgac gcggcaattt aaaccgcgtc aagggttgcc ggatgtttcc atccggcaac
    39361 atcaaattac agcaccttat gcgggccaaa gcattcgtaa tgaatgtttt cctgcttcac
    39421 gcccagatct actaactgtt tcgcggtaaa ctgcatgaag ccaaccgggc cgcagagata
    39481 gaactgcatt gtcggatcgc tgaacgcacc ttccagtttg ctcaaatcca tcagaccttc
    39541 gctatcaaac tgacctttag cgcgatcggc ttcgctcggc tgacgatacc aggtgtgcgc
    39601 ggtaaagcgc ggcagtgact gccccagttc cttaacttca tcggcaaagg cgtgaacatc
    39661 gccattttct gccgcatgga accagttcac ttgtgctgtg tggcctgctt ttgccagcgt
    39721 gtcgagcatt gccagcattg gcgtttgacc aacaccggca gagattaacg tcactggtgt
    39781 gtcatctgcg acagccataa agaaatcacc tgccggagcg accagtttca cgacatcgcc
    39841 aacattggcg tgattgtgca accagttgga tacctgccca ccctcttcgc gtttcaccgc
    39901 aatacgatag cctttgccat ccggtttgcg agtcaaagag tactgacgaa tttcctgatg
    39961 tgggaaacct tccggcttca gccagacgcc gagatattgc cccggacggt attctgccac
    40021 tgcgccaccg tcgaccggct ccagttcgaa gctggtgata agcgcgctgc gcggtgtttt
    40081 agccacaatg cggaaatcgc gagtaccttc ccaaccaccg gctttgctgg cgttttcgtt
    40141 atagatttcc gcctcgcgat tgataaatac attagccagt acaccatagg ctttacccca
    40201 cgcgtccagc acttcctgcc ccgggctgaa catttcgtcc agcgttgcca acaggtgttc
    40261 accgacgatg ttgtactgtt ccggtttgat ctggaagctg gtgtgcttct gcgcgatttt
    40321 ttctaccgct ggcagcagcg caggcaggtt ttcaatatta ctggcgtagg cggcaatagc
    40381 gttaaacagg gcttcacgtt gatcgccatt acgctggtta ctcatgttaa aaatttcttt
    40441 gagttctggg ttatgagtaa acatacggtc gtagaaatgg gcggttaact ttggccccgt
    40501 ttccaccagt aaagggatgg tggcttttac tgtagcgatg gtttgagcgt caagcatatg
    40561 gtcttccttt ttttgcatct taattgatgt atctcaaatg catcttataa aaaatagccc
    40621 tgcaatgtaa atggttcttt ggtgtttttc agaaagaatg tgatgaagtg aaaaatttgc
    40681 atcacaaacc tgaaaagaaa tccgtttccg gttgcaagct ctttattctc caaagccttg
    40741 cgtagcctga aggtaatcgt ttgcgtaaat tcctttgtca agacctgtta tcgcacaatg
    40801 attcggttat actgttcgcc gttgtccaac aggaccgcct ataaaggcca aaaattttat
    40861 tgttagctga gtcaggagat gcggatgtta aagcgtgaaa tgaacattgc cgattatgat
    40921 gccgaactgt ggcaggctat ggagcaggaa aaagtacgtc aggaagagca catcgaactg
    40981 atcgcctccg aaaactacac cagcccgcgc gtaatgcagg cgcagggttc tcagctgacc
    41041 aacaaatatg ctgaaggtta tccgggcaaa cgctactacg gcggttgcga gtatgttgat
    41101 atcgttgaac aactggcgat cgatcgtgcg aaagaactgt tcggcgctga ctacgctaac
    41161 gtccagccgc actccggctc ccaggctaac tttgcggtct acaccgcgct gctggaacca
    41221 ggtgataccg ttctgggtat gaacctggcg catggcggtc acctgactca cggttctccg
    41281 gttaacttct ccggtaaact gtacaacatc gttccttacg gtatcgatgc taccggtcat
    41341 atcgactacg ccgatctgga aaaacaagcc aaagaacaca agccgaaaat gattatcggt
    41401 ggtttctctg catattccgg cgtggtggac tgggcgaaaa tgcgtgaaat cgctgacagc
    41461 atcggtgctt acctgttcgt tgatatggcg cacgttgcgg gcctggttgc tgctggcgtc
    41521 tacccgaacc cggttcctca tgctcacgtt gttactacca ccactcacaa aaccctggcg
    41581 ggtccgcgcg gcggcctgat cctggcgaaa ggtggtagcg aagagctgta caaaaaactg
    41641 aactctgccg ttttccctgg tggtcagggc ggtccgttga tgcacgtaat cgccggtaaa
    41701 gcggttgctc tgaaagaagc gatggagcct gagttcaaaa cttaccagca gcaggtcgct
    41761 aaaaacgcta aagcgatggt agaagtgttc ctcgagcgcg gctacaaagt ggtttccggc
    41821 ggcactgata accacctgtt cctggttgat ctggttgata aaaacctgac cggtaaagaa
    41881 gcagacgccg ctctgggccg tgctaacatc accgtcaaca aaaacagcgt accgaacgat
    41941 ccgaagagcc cgtttgtgac ctccggtatt cgtgtaggta ctccggcgat tacccgtcgc
    42001 ggctttaaag aagccgaagc gaaagaactg gctggctgga tgtgtgacgt gctggacagc
    42061 atcaatgatg aagccgttat cgagcgcatc aaaggtaaag ttctcgacat ctgcgcacgt
    42121 tacccggttt acgcataagc gaaacggtga tttgctgtca atgtgctcgt tgttcatgcc
    42181 ggatgcggcg tgaacgcctt atccggccta caaaactttg caaattcaat atattgcaat
    42241 ctccgtgtag gcctgataag cgtagcgcat caggcaattt ttcgtttatg atcatcaagg
    42301 cttccttcgg gaagcctttc tacgttatcg cgccatcaaa tctgtcgtaa ctgcgcctca
    42361 acatacaaat agccaattcc cagcacctgt tgtgcgcggc ttaattgccc aaagccaatt
    42421 tgcgtcgctt tcacgctcgg tccttcgtcg cggtcgaacg ggttaaatcc tgtctggcga
    42481 ataatagtat taagccagtt ctcaccaaag gcacaactgc gaccgtacaa ccaaatctga
    42541 ttaatattga ggatgttgag gaagttatac agactcaaac caatggcatt ggcagagcga
    42601 tcaacccagc tggtgatcca cggttctcca ctttgccagg cagcgattaa ctgcgctgta
    42661 gtcagttttt caggatcaag ttgagtacta accggttgtg attttagcca tacccgcgcc
    42721 tgttttttta atgcgcttaa cgaggcgacg gtttccaggc agccataacg tccacagtcg
    42781 cagacgacgc catccggatt aacgatggtg tgaccaatct gtccgctacc atacaaactg
    42841 ccgcgataaa tttgctcgtt aatcacgaac gacgagccaa tgccgtaatc aacgttgatc
    42901 acgcagaaat cccgttcctg cgaattattt tgccatttct ccgccagcgc cagcatcacg
    42961 cagtcattat cgaccatcac ccgaatgccg agcttctctt ccagcagata tttcacctcc
    43021 accggcgttg tccacggcgc ttgcggcatg gtttgcgaca cgccggtcac aggatcaacc
    43081 tgaccgtgga ttgccaacgc caggttgatg gtatggtccg gccacaattt acggtggcga
    43141 tgccagcatt tttcgatttc ggacagcagc gcctgcggcg tcggtgcatc aatctgtaaa
    43201 tactcaaatt cacctttcgg acttaaacaa gcattagcaa cctgacactc aatactggtg
    43261 ggcgtcacgt tcaggcacag cgtccagtca ccttccggcg caatcagcca tgtaccgcta
    43321 ctatgcccgc gcgtctggct ttcatcgtca atattcacca cccgtttttc gctttccagt
    43381 tcctgcaaaa tattactgac tgccggaatc gaaatttgcg ccagccgggc cagcgttgat
    43441 ttattggcgc gcttttgccg gtacagcagt tccagaatca cgcatttgtt atggtggcga
    43501 atctgttgat tattaatgca ggctctcatc ttcactaaac cttgtttagt taattgcgtg
    43561 attattccgc gaaaacattg ccagattgtt caatacactg ccacaaatct tttaattcag
    43621 tatgtcttgt taatattgag ggcaccatga ctccagtaaa agtgtggcaa gagcgcgttg
    43681 agatcccgac ctatgaaacc gggccgcagg atatacatcc catgttcctg gaaaatcgcg
    43741 tttatcaggg atcgtccggc gcggtttatc cctacggcgt gaccgatacg ctgagcgagc
    43801 agaaaaccct gaaatcctgg caggcggtgt ggctggaaaa cgactacatc aaagtgatga
    43861 tcctgccgga actgggcggt cgggtgcatc gcgcgtggga taaagtgaaa cagcgcgatt
    43921 ttgtttatca caatgaagtc attaaacctg cgctggtggg gctgctgggg ccgtggattt
    43981 ccggtgggat tgagtttaac tggccgcaac atcatcgccc gaccaccttt atgcccgttg
    44041 atttcaccct cgaagcccat gaagacggtg cacagacggt gtgggtaggc gaaacggagc
    44101 cgatgcatgg tttacaggtg atgacaggtt tcaccctgcg ccctgaccgg gcggcgctgg
    44161 aaatcgccag ccgcgtctat aacgggaacg ccacgccgcg tcatttcttg tggtgggcca
    44221 acccggcagt gaaagggggg gaagggcatc agagcgtctt cccgccggat gtaacggcgg
    44281 tgtttgatca cggcaaacgg gccgtctccg ctttccccat cgctaccggc acttactaca
    44341 aagtggacta ctccgctgga gtggacattt ctcgctataa aaatgtgcct gttccaacct
    44401 catatatggc cgaaaaatcg cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg
    44461 gcggtttgct gcacgttgcc aaccaccata ttgcgccagg taaaaaacag tggagttggg
    44521 gacacagtga atttggccag gcgtgggata agagtctgac cgacaataac ggcccgtata
    44581 tcgaactgat gaccggtatt tttgccgata accagcccga ttttacctgg cttgatgctt
    44641 acgaagagaa gcgttttgag cagtatttcc tgccttatca ttctttgggc atggtgcaaa
    44701 atgcctcccg cgatgcggtg ataaaactcc agcgtagtaa gcgggggatt gagtgggggc
    44761 tgtatgccat ctctccgttg aacggatacc gcctggcgat ccgcgaaatc ggtaaatgca
    44821 acgcgttact tgatgatgcc gtggcactga tgcctgcgac cgccatccag ggcgtgttgc
    44881 acggtatcaa tcctgaaagg ctgaccattg agctctccga tgccgacggc aatattgtac
    44941 tgagttatca ggaacatcag ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac
    45001 tggcagcaca agacattacc agtacagatg aagcctggtt tatcggtcag catctggagc
    45061 aatatcatca cgccagccgt tcaccgttcg attactacct gcgcggcgtg gcgctggacc
    45121 cgctggatta tcgctgtaac ctggcgctgg cgatgctgga atataaccgc gcagatttcc
    45181 cgcaagcggt ggcgtatgcc actcaggctc tgaaacgcgc acatgcgctg aacaaaaatc
    45241 cgcagtgcgg acaggcgagt ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc
    45301 aacaagccga agaggatttc tggcgtgcgg tctggagcgg caacagcaaa gccggtggct
    45361 attatggcct ggcacgactg gcggcgcgta atggtaactt cgacgctggt ctggattttt
    45421 gccaacaaag tcttcgcgcc tgcccaacca atcaggaagt gctttgcctg cataatctgc
    45481 tgctggtgtt aagtggtcgt caggacaacg cgcgtgtgca gcgcgagaaa ctgctgcgcg
    45541 attatccgct gaacgccact ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag
    45601 ctctcgcgca gtggcgcggt ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg
    45661 ggcaactgat taactgggga atgcccaccc tggcggcaga gatgctgaac gcactggact
    45721 gccagcgcac gctgccgctt tacctgcaag ccagcttgct gccgaaagcc gaacgtggcg
    45781 aactggtcgc aaaagccatt gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg
    45841 aagaagtggc ggcgctggag agtattgaag agtgctggtt tgctcgccat ttactagctt
    45901 gcttctacta caacaaacgt agctacaaca aagccattgc cttttggcaa cgttgcgtag
    45961 agatgtcgcc ggagtttgcc gacggctggc gcgggttagc gatccatgcg tggaataagc
    46021 aacacgatta tgagctggcc gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg
    46081 atgcacgtct gcttttcgaa cgggatttgc tggataagtt aagtggagcc acaccggaga
    46141 aacgactggc gcgtctggaa aataatctgg aaattgcgct gaaacgcgac gacatgaccg
    46201 cagaactgct caatttgtgg catctcacgg gtcaggcaga caaagcggcg gacattctcg
    46261 ccacgcgcaa attccacccg tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc
    46321 tcaaccagtt attacgcgcc tggcagcatc ttgatgccag acagccgcag caggcctgcg
    46381 aactgcttca tgccgcgctg cattatccgg agaatttaag cgaaggccgt ttaccggggc
    46441 aaactgataa cgacatctgg ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa
    46501 ctgaagcgat gcgttgttta cgtctggcgg cgaccggcga tcgcaccatt aacatccaca
    46561 gttattacaa cgatcagccg gttgattatc tcttctggca aggaatggcg ctgcgactgc
    46621 tgggtgaaca gcaaaccgca cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga
    46681 tggcgaaaac cagtattgag gcggatttct ttgctgtttc acaacctgac ctgttgtcgc
    46741 tgtatggcga tttacaacag cagcataaag aaaaatgcct gatggtggcg atgctggcgt
    46801 ccgcgggact cggggaggtt gcgcaatatg aatctgctcg cgctgaattg acggcgatta
    46861 atccggcctg gccgaaagcg gcattattca ccaccgtgat gccttttatt tttaactacg
    46921 ttcactaaac cctattaact gtaatatttg agcggcatgc gctgccgctc atcacaaaat
    46981 aattagaata agccgttgtt aataaaaacc ctacaggagg cactatgcct aaaaaaatga
    47041 gaacaacccg taatctatta ttaatggcta cgctattagg tagcgcacta tttgccaggg
    47101 ctgcggataa agaaatgacc attggtgcaa tataccttga tacccaggga tattacgctg
    47161 gagtgcgtca gggcgttcag gatgcggcaa aagattcttc agtacaggta cagttaattg
    47221 aaactaacgc ccagggtgat atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc
    47281 gtaatgtcga tgccattatt ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc
    47341 gtcgcgccag tgaagcgggt attccggtga tttgctacaa cacctgtatt aatcaaaagg
    47401 gtgtcgataa atatgtctcg gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac
    47461 tgggtaacgc tgccgccgat tattttattg ccaataaaat tgaccagccg aaaattgccg
    47521 tcatcaattg cgaagccttt gaagtttgtg tgcagcgacg taaaggattt gaagaagtat
    47581 taaaatcccg cgttcccggc gcgcaaattg tcgctaatca ggaagggact gttttagata
    47641 aagcgatttc cgttggtgaa aaactgatta tctccacgcc ggatctcaac gccattatgg
    47701 gggagtcggg cggtgcgaca ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg
    47761 gaaaaattgc tgttttcggt tcggatatga caaccgaaat tgctcaggag ctggaaaaca
    47821 atcaggtgct gaaagcggta gtggatattt ccggtaagaa aatgggcaat gctgttttcg
    47881 cgcaaacatt gaaggttatc aataaacaag ccgacggtga aaaagtgatt caggtgccaa
    47941 tcgatctcta taccaaaacg gaagatggta aacagtggct ggcaacgcac gttgatggtc
    48001 tgccctaatc gctgattcgc agaggtccct atgttcacgg caacagaggc agtcccggta
    48061 gcaaaagtgg tggcaggaaa taagcgttac cccggcgtcg ttgcgctgga taacgttaac
    48121 ttcacgctca ataaaggcga agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg
    48181 actctcattc gaatgcttac cggcagcgaa cgcccggata gcggtgatat ctggattggc
    48241 gagacacgac tggaaggtga cgaatatgcg tttgatttct tctgtctgga accgatgagc
    48301 catgcgccgg atgatcatca tcgcccggaa gggggtgatc tcattgcgct agcaccaggg
    48361 gaatcaacaa cttcagagat gtcgttgcgg gtagagtggc tgtaatttat aaaatgccgg
    48421 ataaggcgtt cacgccgcat ccggcatctt tcctaacgcc gatcgagaga aattgccccc
    48481 ggtccggtaa ttgccaacag caggaatgcg ccagcgatac tgacattctt ccagaaatta
    48541 atcatatttg gcccgacggc atcgccggtc atatcccagt aatggtgacc aatcaccgcc
    48601 gtacccagcg tgtagaaaat aaacagcacc gccagcggac gggtgaaaaa gccaagcaca
    48661 attaatatcg cggcgggcac ttccataact accgcaataa tcgctgccag catcggcatt
    48721 ggcgcgccca acgaggccat atattggacc gtaccgtcaa agcccatcat tttgggaaaa
    48781 ccaaaaataa tgaaaattaa gaccacggcg atacgggcaa ttaataacaa aacggggcgg
    48841 gcagctccaa aatcaaaata acgtagtgtg ttcataacca atccctttgc aagataatgt
    48901 gatttaaacg taatacacac tcttgcacct cgccacgccc caggcattga gatattcgct
    48961 aattatcctg gttacagtga tttaagcgcg atgttctcat ctatcagcag tttcgcatca
    49021 aacgttttgc cgctctggat ccatttgcgg attgagcgaa tctcacgtcc ctgattcagc
    49081 gttaccgcac cgataagcac gccgttttgc agattaaacc aaatcgcctt ctgagtttcc
    49141 gggttgccac gacaaagcca gtcatcacca cgcatatcgc caataaactg taagttatca
    49201 ctgtactgat cgctccagaa ccacggcggc ggcagtagcg gaagcggtag ccctaacatt
    49261 gcggcagcgg caatttgcgc ctggttattg gcgttttccc agctttcgca gcggtgtagt
    49321 gcaccattat caagacgagt gattgccaca tcgccaccgg caaagatcgc gggatcgcag
    49381 gtgcggcaag cctcatcaat gacaatgcca ttggcagtat caaggttggc ttcgcgagcc
    49441 agttggtcgt tggcgctgat accaataccg taaatcacca catcggcctg aagcgtttcg
    49501 ccactttgca gcgtcagttc tactttttca ccatcgacca catgttcaat ggcattattg
    49561 agcagaatgc gcacaccagc ctgctgatgg cgctgtaaaa gatagcgttg cacgggcggt
    49621 ggtgcattac ggcccatgac ggttgccgcc agttcaatca ctgtcacctt acatccgcgc
    49681 tgcgtggcgc tggcagccag ttccagacca atagttccgg caccgacaat cacgactgac
    49741 cgttcgggct gcagaacttc tcgcagtctg gcggcatcac cggcatggcg cagggtaaag
    49801 cagcgttctc ccagtgcatc aagcaacggc agcggtcggg ctgccgcgcc ggttgctata
    49861 aaaagctgat cccagtgcca gctttcgccg ttggttaaca ctaactctcg tgtgtcgcga
    49921 cccagcgttt tgatggttac accggaatgc agatgaacat tgttttcctg ccaccagtta
    49981 gcgggtaaca cctgctgtaa ctgcggggaa tcttccagca acatggattt cgagagtgga
    50041 ggtcgttcat aaggaagatg tcgctcatcg gaaaacagat gcagctcacc ggtgaaccct
    50101 tgctggcgta gcgaggccgc agccattgcc gccgcttgcc cgccaccgac aataatgatc
    50161 gttttttctt tcatcgtgct tccttaaaga tccagcccag ccgctacgtg gcgaatgccg
    50221 cgaatcgcta aacccgcatc agcgttgatc atcacaccgc ttaatgcgcg attattgcgc
    50281 cgcgatgcca acatcacata cggccccgta aaatccgccg gttgcgggaa aaattgcagc
    50341 ggtaaaatgg cggcaatttt ctccggcgtc agagactgca ttatcgaggt ttcactttgc
    50401 ccgagcgcct gtgggccgcg caggtcgctg gccataccac acgggccgac gccattcacc
    50461 cgcactttcg gtgccagttc ataagccagt tggcgaataa gtccggttgc ggcatgttta
    50521 ctggcggtgt acagcgggcc accgccgcca ggataccagg cggcatttga cagtgtgaaa
    50581 atcatgctgc cttcactggc gattaacgcc ggagcgcagg cttttgcgcc cagcaggtaa
    50641 ccgagaacat tgacgttaaa cagctcgtgg aagccggttt cgagcgtctc tgcgggagta
    50701 ttaaccagtg aggcattgtg atcccagata cctgcattgc cgataaaaca atccagcttg
    50761 ccggaacgag tcaggatctg atcgaccgcg cgttgataat cggcataaca ggtcacgtta
    50821 ccttccaccg ccagaatatg ttcgccaaat cgctgacgca gactggcgac ttttgccgcc
    50881 gacagttcca gcgtggcaac ctgcgcgcct tcttcgataa atcgctcgac cagcgccagc
    50941 cctaatcccg atccgccgcc ggtaataaaa atggactcgt tatgcagatc gctcatggct
    51001 gcgcctccgg taagtcgatg aaaatgtcac caccttcaac gtgtactgga taagtggtga
    51061 gcggatcggt ggcgggcagg cataacgctt tccccgtttt caggcaaaaa ctggcggcgt
    51121 gtagcgggca ctccaccgtg gcgtcatctt ccagataccc ttctgacatt gacgcattac
    51181 catggctgca acgatcgtta atggcataaa actcgccgcc aacgttgaac agggcgatga
    51241 cgggcgaggt atcgatccgg agagcctcac cctccggcac atccgcaacg ggacacgcat
    51301 aaattcgatt catcagaaca gtacactcag gttatgggaa gtgattaccg cttgatccag
    51361 gacgatatcc cgttccagca agcgccagtt gtcatcttcc agacggcgaa ctttgtcgaa
    51421 acgcgttcca acatagaatg tttcatcgcg ctctttttgt gcccgataaa gcagataatt
    51481 tacccgcaca gcaaatacgt ttgggatgtc ggtttcgctt atctggcagt tgctgattaa
    51541 gtgacgggtg cgtgacggcg gctcttctgc ccaggccatg cccgtttcca gacgggcgat
    51601 tcgccgctcc agctggtctt tggtgtcatt aaaaatccag gttgtcggtg gctggacgcc
    51661 tttgcggcgg tcgcgtgttt gcgcgttaac tgtggtgcgc atggtgtaac gaatctcttc
    51721 gtcgagctgc gccagccagt cacgaaattt ccagtcgtcc agtaagctgg cttcgtgaaa
    51781 gagaaactgg ctaatgcggt gatgtaactc tagtgaaact tgcgcactca tttcatcacc
    51841 tcctgctggt aagcggcggt tttatcgagc acttcctgcc agctttcgct actcagaaga
    51901 tcggcccagc gttggtacat tccacgagcg gcagtttctg aaaagatata gttagtaatg
    51961 ccaggaatgc cgtcgtcgcg gcgcttttcc tgaccaagcc ccatttccag acacagtttg
    52021 ctgttgcggg cgcggtgacc tttcagcaat ttctggattt cacaccagtt ctccgagtca
    52081 tcctgctcga gaaaaccagc aggaccaaaa gcacgagtgg cgctgttttc aaaagcggct
    52141 ttaacttcat cggaggcggc tttgtcagta atacagaacg cccacacttc aacttgatca
    52201 gggccgcgcg gatgtcagac gcggagcgtg gcagtgccgt tgagccatga aagcgtgggg
    52261 aaaatattgt tatgacccgc caggcgcagg gcgcgaactt cacctaaacg ttgttctgct
    52321 tcggcatagg tttcgcggta atagcttgaa actgcgccat cgacccagac attagcatcc
    52381 ggtttttcag taaagaagaa accgctaccg tgaccgtcct gaccaaattg cagcgcatct
    52441 ttggcggttt cccacaccgg gcgggcggtt tgtccatcac cgaggcgctt atcgctgcca
    52501 tcatctttcg cccctaatac ctgaacggca gaagcatggc tgaacagagc atgatactgg
    52561 tcactggcga actgctctgc cgggaatttc cagttacagt tgatcaccca cttttgtacg
    52621 ccgccgacaa tttcggtgcc gccttcgcga cgatccagca tgccatccag ataccaggca
    52681 atgtcaccca ggtaatcacg caggcccggt gcgctggtat cccagttgcc aaaaatcagc
    52741 cctttataac tctccacaca aggaacttcg tttagtcccc agtgggattt acacaaccct
    52801 tgtgggtagg cgcgaggttc cagcggtaca tcgatcaact cgccgttaat gccataagac
    52861 cagccgtgat acgggcaggt aaaggcgcga gtgttgccgc aatctgcata actcacacgc
    52921 atggcccggt ggcggcattg gttgagaaac gccttgatgc tgccgtcttt ctgacgcact
    52981 acgacaaccg catcttctcc catgtaggtg ttaaagaaat caccgggttt tgggatctgg
    53041 ctttcgtggg cgagaaatag ccagcaacga ccgaaaatac gctcaagctc cagttggtaa
    53101 atgtccgggt cggtataaat acgcggagtg acccgaccgt tttgggtgtc aatcagttgg
    53161 taaatgttca aatctgaggg tgtggtcata ttttttaatc ctgaaagata agatgtattg
    53221 accttctatt tttgtgccat aaggatgttg ctggttggtt gctaatatgt gaaatagtta
    53281 atttctacca gctgagacgt tttttcgata acaaggggaa ggtgatggaa ctacggcatt
    53341 tacgctattt cgttgcagtg gcgcaggcac tgaactttac ccgtgcggcg gaaaaactgc
    53401 atacctcaca gccttcgtta agcagccaga tccgcgatct tgaaaactgt gtcggtgttc
    53461 cgttactggt gagggataag cgcaaagtcg cgttgacggc ggcgggagaa tgttttctcc
    53521 aggacgcgct ggcaatcctc gaacaagcgg aaaatgccaa attacgggcg cggaaaattg
    53581 ttcaggaaga cagacaatta accattggct ttgtgccatc ggcggaagtg aatttactgc
    53641 caaaagtatt accgatgttt cgtctcagac agccagacac cttgattgag ctggtgagtt
    53701 taatcaccac gcaacaggag gaaaaaattc gccgtggtga actcgacgtc ggcttaatgc
    53761 gccatcctgt ttatagcccg gagattgatt atctggagct ttttgacgaa ccattagtgg
    53821 tggtgttacc ggttgatcac cctttagcac atgaaaaaga gatcaccgcc gcgcaactgg
    53881 atggcgtgaa tttcgtcagt accgatccgg tgtattccgg ttcgcttgcg ccgatcgtta
    53941 aagcgtggtt tgcgcaagaa aacagccagc caaatatcgt ccaggtggca acgaatattc
    54001 tggtgaccat gaatctggtg ggcatggggc tgggcgtcac tttgataccc ggttatatga
    54061 ataattttaa taccggacag gttgtttttc gtcccattgc cggaaatgtg ccttccattg
    54121 ctttactgat ggcgtggaag aaaggggaga tgaagccggc attacgcgat ttcatcgcca
    54181 ttgtgcagga acgtttggca agcgtaacgg cataattacg cattattttt gcaacatgcg
    54241 tcgcggatct cagtttttta gtgcttgtga actggcgtta cagacatcgg gaagcaggct
    54301 cgaatattct tatttttcaa cttgcaccaa cattcgatta accccagact aacgcctcct
    54361 gacgggaggg actcatggtt ttgcaatcca cgcgctggtt ggcgctcggc tatttcacat
    54421 acttttttag ttacggcatt tttctacctt tctggagcgt ctggcttaaa gggattggtt
    54481 taacgccaga aaccatcggc ctgttattgg gggcaggtct ggttgcccgt ttcctcggga
    54541 gtttgctcat cgcgccccgc gtcagcgatc cttcccgcct gatttccgcc ttgcgcgtgc
    54601 tggcactgct gacacttctc tttgctgtcg ccttctgggc gggggcgcac gtagcgtggc
    54661 tgatgctggt gatgattggc tttaacctct ttttctcacc actggtaccg ttgaccgatg
    54721 cactggcgaa tacgtggcaa aagcagttcc cgcttgatta cggcaaagtg cgactgtggg
    54781 gctcggtggc gtttgtcatt ggctcggcgc tgacgggcaa actggtcact atgtttgatt
    54841 atcgggtgat cctcgcgctg ttgacgttgg gcgtggcatc catgctgctc ggctttctca
    54901 tccgtccgac gattcagcca caaggggcaa gtcgccagca ggagagcacc ggttggtctg
    54961 cgtggttggc gctggttcgc cagaactggc gctttctggc ctgcgtttgt ttattgcagg
    55021 gggcacatgc ggcctattac ggttttagcg ccatttactg gcaggcagct ggctactcgg
    55081 cctcggcggt ggggtatttg tggtcgctgg gcgtggtggc ggaagtcatt atctttgcgc
    55141 tgagtaataa acttttccgc cgttgtagtg cacgcgatat gctgttgatc tcggcgattt
    55201 gcggcgtagt gcgctggggc attatgggag caactacggc gttgccgtgg ttgatagtgg
    55261 tgcaaattct gcattgcggc accttcacgg tctgccacct ggccgccatg cgttatattg
    55321 ctgctcgcca gggtagcgaa gtcatccgtt tacaggcggt ttactctgcc gtcgcgatgg
    55381 gcggcagtat cgctatcatg accgttttcg ccggtttcct gtatcaatat ctgggccacg
    55441 gcgtgttctg ggtaatggcg ctggtggcgc ttccggcaat gtttttgcgc ccgaaagttg
    55501 ttccctcatg ctgattcgag cattttgcgg atctgctcct gttgatgtgt tgtcagcgtc
    55561 aggtccgtat agatcagcgg tggtgaaaag agcggtaatg gcatggtata aggcgcaata
    55621 atcagtgccg cgccacgcgg agcgcctgtc tgcaaaaatg cctttaccga catatgctta
    55681 atgttgagcg gcagtaacgt tagttcgcgt aactgctgct caatctgcgc ttctcgctcg
    55741 ctatcattcc cggtaagaag aataatctgt ttttcatgca gatcgttttc ctgcattagc
    55801 caggcaccga aaatcaccgc gaccagacca ctttcctcat cagaaagatg gacgccgtat
    55861 tcactttcaa atccggccag cgccgcgcgg gtggtgcgca ccaggcgtgg gtacagtctg
    55921 gcgaactctt ccggcagggt attatcaata ccgatggcaa acaaactgcg atttaacgcc
    55981 tgggcgaggt gggtataaag ctgatcacat aacccctgtt catcataaaa acgaacattt
    56041 cccagctcac gaaaatggtt taccagacgt ttgatagact gacgcaattg tctgtcccgc
    56101 tgatgcgcat cccgcaatgg atcgggaacc cgcaacatgg aaaaaagtag tgccataaac
    56161 agtggctcat caggtggtac aggctggagc gcccgacgct gccagtggcg tccaatttcc
    56221 tgcgctacct gaaattcaag gcaggattcc gcccagcgac gttggagcgg attaaactga
    56281 ggcgtaattc cagcgtggtg ctgcagcaaa caatattgca gatacagaca caggaaatga
    56341 atatcgcgcg attcaaagcg tttttgcagc cgtcgggagc agagattcac taacgcttgt
    56401 agattggtat cgtcgtaaaa gttacgcgca attccgcgcc gcttcagttc actcttcagg
    56461 gcgggggtga attggctggt aataaatgac ggacacagac ggaaaccacg tcgtagccag
    56521 tgaaataaac acaaacgttg gttaagcact gtaccttcaa cccggtagct accgtcataa
    56581 ccagtcgtca gtgtgagttg atgatagcgc aggatctccc gtcctgtttc gctgatatca
    56641 agactggcaa tatcatcatc cacaccatta agctcgctga aggttgccat ggtggcggtc
    56701 aaccccggct gaaagagcgt cagcaagatc tggcagcggc gctggggagc cgaaaggaca
    56761 gatggtggag caagcgtagg catcatctgt agatctccca taagcataat tgataagaat
    56821 agctaaagtt tgctcaagga aatggcgaag ggaatgctaa tcatcagaaa tgttggcaag
    56881 cgtcacagaa tttttctcat cttaaactgt tctgaccagt ggattagcat gtcttaaggt
    56941 ggttttgtga atgattatcg tgctagcgat catgggttag ttctgcggat tcaacgcgct
    57001 aaggataaag tccaccatct gttcgcgata aacatcgccg tgacgatcgg aaaaagcatc
    57061 aatgtgttcg ccatccggga ttagaatcaa ccgttttggc tcttttgcca ggctatacaa
    57121 cttttcgctg tgctgccatg ggataacgtg atcggcttta ccgtgaatga gtaaaagcgg
    57181 gatcgggctg acgctggcga tgtaattttc gccactgtaa ctctcatcaa gtaagtagcc
    57241 actgccgggg atcatctggt tggcgatggt tgcataagag gcaaatgtgg agtcgaggat
    57301 caccgcacgt atgccttcac gatcaccctg accaataaca gcaagaatat tcgccccgcc
    57361 aatactctgc ccgaacagca ccagccgttg tgggtttaca tcactgcgat ggcgcaccac
    57421 attgatggcg ctttgcgtat cgtccagcaa tccggcctgg gacggcgtgc cttttgattt
    57481 accaaacccg tgataatcaa acataaaaac gttgaaatta cgctcgggta accaactgac
    57541 cagcggccag tgggcggaca tatttccggc attgccgtga gcatgaatga tggttgcgat
    57601 agcgttgtca gcagggcccg tcgaaaaagg aataaaccag ccttgcagac gagtaccgtc
    57661 cttagccgta aattcgacgg attccgccga ccaggggtcg ggaccgtaaa gtttatcgtc
    57721 aggataatag aagaagttga tggcaacgcg cggcactaaa taaacgcaga aggcaacaac
    57781 aaagagaata aacagaattt tgagaacgcg tttgttcact ggcagtgcca taacctttcc
    57841 ttgcgacttc tttatcatcc attaatcggg gaaagatggt gggagagtga cgaaagaaag
    57901 taaagcagtg aggaagtatt tcggaatagc gcctgaaagg cgagggcggg tgagtgatat
    57961 cacccgcctg agtcattaac gcttcagagc gtcgcttaac tcgtcacgca tgttcgccag
    58021 catggcttta acaacgcgcg ggttaccagc aacgatgtta ccggtcagca tatagttatg
    58081 accaccggtg aagtcgctga cgatgccgcc cgcttcacga accagcaact cgcctgcagc
    58141 gaagtcccac gggcgcagac cgatttcaaa gaaaccgtca acacgacccg cagcgacgta
    58201 agccagatcc agcgccgcag aaccggtacg acggaagtct gcacattcgt tgaatagttt
    58261 gccgacgatg ttgatgtagg tagtggcgta ctgtttggct ttgaacggga agccggtcgc
    58321 cagaatagta ccgtcgagat cgcgagcggt gctgccgcgc agacggtagc cgttcagctg
    58381 tgcgccctga ccgcgagtgg cggtgaacag ttcgttacgc ataggatcgt ataccacagc
    58441 aacttcggtg cggcctttga tacgaacggc gatagatacc gcgaagtgcg gcagacgttt
    58501 gataaagttg gtagtgccat ccagtggatc aataacccat tgaacatcct gatcagtacc
    58561 ttcaagttca ccgctttctt cggtgatgat ggtgtgctgt gggtaagatt tacgaatcgt
    58621 gtcgataatc accgcttcgg cagctttatc tacgttggtc acgaaatcgt tactgccttt
    58681 ctggctcgct tctacagcgt ccggggtttc atagtttttg gcaattaaat tacccgcctt
    58741 gcgcgctgcg cgcacggcga tgttcagcat cggatgcatc ggtctctctc actggatgtt
    58801 aaagaacggg aaaacggcgc agagtatagc agcgagatcg gcatctgtct tcgttttatg
    58861 ataagatgcg cgaataatct ttagacgaag aaagacaatg ctgcaaaata ttcgaattgt
    58921 gctggtggag acgtcacaca ccggcaatat gggttctgtt gcccgtgcca tgaaaacaat
    58981 gggattaacc aatctgtggc tggttaatcc actggtgaaa cccgactccc aggcgattgc
    59041 cctggcagca ggggccagcg atgtgattgg taatgctcac atcgtcgata cgctcgacga
    59101 agcgttagcc ggttgtagcc tggtggtggg caccagcgca cgttcccgca cgctgccatg
    59161 gccgatgctc gacccgcgcg aatgcggcct gaaaagcgtc gctgaagcgg caaatacccc
    59221 ggtggcgctg gtgtttggtc gcgagcgcgt cggcttgacc aatgaagagt tgcagaaatg
    59281 ccattatcat gtcgcgattg cggctaaccc ggaatacagc tcgctgaacc tggcgatggc
    59341 ggttcaggtt attgcctatg aagtgcgcat ggcctggctg gcaactcagg aaaacggcga
    59401 gcaggtcgag catgaagaga cgccgtatcc gctggtcgat gatctggagc gtttttatgg
    59461 tcatctggaa caaacgctgc tggcaaccgg ttttatccgt gaaaaccatc cggggcaggt
    59521 gatgaataaa ttgcgccgtc tgtttacccg tgcgcgcccg gaaagccagg agttgaatat
    59581 cctgcgcggg attctggctt ctattgagca gcagaataaa ggtaacaagg ccgaataa
//
LOCUS       DAZFZC010000019        54194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFZC010000019 DAZFZC010000000
VERSION     DAZFZC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.019"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..476
                     /locus_tag="ACHKXP_001380"
     CDS             <1..476
                     /locus_tag="ACHKXP_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO7646427.1"
                     /translation="YAPLGLYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIV
                     RLDAFKASKDIQFAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQ
                     QATNFYDFACYAKAKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(623..2785)
                     /gene="uvrD"
                     /locus_tag="ACHKXP_001381"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(623..2785)
                     /gene="uvrD"
                     /locus_tag="ACHKXP_001381"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO7646428.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2869..3585)
                     /gene="yigB"
                     /locus_tag="ACHKXP_001382"
     CDS             complement(2869..3585)
                     /gene="yigB"
                     /locus_tag="ACHKXP_001382"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO7646429.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3585..4481)
                     /gene="xerC"
                     /locus_tag="ACHKXP_001383"
     CDS             complement(3585..4481)
                     /gene="xerC"
                     /locus_tag="ACHKXP_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO7646430.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4478..5185)
                     /gene="yigA"
                     /locus_tag="ACHKXP_001384"
     CDS             complement(4478..5185)
                     /gene="yigA"
                     /locus_tag="ACHKXP_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO7646431.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5182..6006)
                     /gene="dapF"
                     /locus_tag="ACHKXP_001385"
     CDS             complement(5182..6006)
                     /gene="dapF"
                     /locus_tag="ACHKXP_001385"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO7646432.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6043..6246)
                     /gene="yifL"
                     /locus_tag="ACHKXP_001386"
     CDS             complement(6043..6246)
                     /gene="yifL"
                     /locus_tag="ACHKXP_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7646433.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6405..6547
                     /locus_tag="ACHKXP_001387"
                     /pseudo
     CDS             6405..6547
                     /locus_tag="ACHKXP_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            6709..7029
                     /gene="cyaY"
                     /locus_tag="ACHKXP_001388"
     CDS             6709..7029
                     /gene="cyaY"
                     /locus_tag="ACHKXP_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO7646434.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7069..9615)
                     /gene="cyaA"
                     /locus_tag="ACHKXP_001389"
     CDS             complement(7069..9615)
                     /gene="cyaA"
                     /locus_tag="ACHKXP_001389"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO7646435.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLENWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10002..10943
                     /gene="hemC"
                     /locus_tag="ACHKXP_001390"
     CDS             10002..10943
                     /gene="hemC"
                     /locus_tag="ACHKXP_001390"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO7646436.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDSRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            10940..11680
                     /gene="hemD"
                     /locus_tag="ACHKXP_001391"
     CDS             10940..11680
                     /gene="hemD"
                     /locus_tag="ACHKXP_001391"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO7646437.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            11702..12919
                     /gene="hemX"
                     /locus_tag="ACHKXP_001392"
     CDS             11702..12919
                     /gene="hemX"
                     /locus_tag="ACHKXP_001392"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO7646438.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            12922..14118
                     /gene="hemY"
                     /locus_tag="ACHKXP_001393"
     CDS             12922..14118
                     /gene="hemY"
                     /locus_tag="ACHKXP_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO7646439.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRHDGLMLTL
                     QNNPPQ"
     gene            14797..16452
                     /gene="aslA"
                     /locus_tag="ACHKXP_001394"
     CDS             14797..16452
                     /gene="aslA"
                     /locus_tag="ACHKXP_001394"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO7646440.1"
                     /translation="MEISFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVIFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKTLEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(16620..17855)
                     /gene="aslB"
                     /locus_tag="ACHKXP_001395"
     CDS             complement(16620..17855)
                     /gene="aslB"
                     /locus_tag="ACHKXP_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7646441.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAEHHFLVGLSLDGPPEIHNQYRVTKGGRPTHKLVMRALTFL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMSDLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(18002..18078)
                     /locus_tag="ACHKXP_001396"
     tRNA            complement(18002..18078)
                     /locus_tag="ACHKXP_001396"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18042..18044),aa:Pro,seq:tgg)
     gene            complement(18121..18207)
                     /locus_tag="ACHKXP_001397"
     tRNA            complement(18121..18207)
                     /locus_tag="ACHKXP_001397"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18171..18173),aa:Leu,seq:cag)
     gene            complement(18228..18303)
                     /locus_tag="ACHKXP_001398"
     tRNA            complement(18228..18303)
                     /locus_tag="ACHKXP_001398"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18268..18270),aa:His,seq:gtg)
     gene            complement(18362..18438)
                     /locus_tag="ACHKXP_001399"
     tRNA            complement(18362..18438)
                     /locus_tag="ACHKXP_001399"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18402..18404),aa:Arg,seq:ccg)
     gene            complement(18541..19926)
                     /gene="yifK"
                     /locus_tag="ACHKXP_001400"
     CDS             complement(18541..19926)
                     /gene="yifK"
                     /locus_tag="ACHKXP_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7646442.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(20117..20857)
                     /gene="wecG"
                     /locus_tag="ACHKXP_001401"
     CDS             complement(20117..20857)
                     /gene="wecG"
                     /locus_tag="ACHKXP_001401"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO7646443.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIIMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20860..22212)
                     /gene="wzyE"
                     /locus_tag="ACHKXP_001402"
     CDS             complement(20860..22212)
                     /gene="wzyE"
                     /locus_tag="ACHKXP_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO7646444.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(22209..23288)
                     /gene="rffT"
                     /locus_tag="ACHKXP_001403"
                     /gene_synonym="wecF"
     CDS             complement(22209..23288)
                     /gene="rffT"
                     /locus_tag="ACHKXP_001403"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO7646445.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(23285..24535)
                     /gene="wzxE"
                     /locus_tag="ACHKXP_001404"
     CDS             complement(23285..24535)
                     /gene="wzxE"
                     /locus_tag="ACHKXP_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO7646446.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(24537..25667)
                     /gene="rffA"
                     /locus_tag="ACHKXP_001405"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(24537..25667)
                     /gene="rffA"
                     /locus_tag="ACHKXP_001405"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO7646447.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(25672..26346)
                     /gene="rffC"
                     /locus_tag="ACHKXP_001406"
                     /gene_synonym="wecD"
     CDS             complement(25672..26346)
                     /gene="rffC"
                     /locus_tag="ACHKXP_001406"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO7646448.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(26324..27205)
                     /gene="rfbA"
                     /locus_tag="ACHKXP_001407"
     CDS             complement(26324..27205)
                     /gene="rfbA"
                     /locus_tag="ACHKXP_001407"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO7646449.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERDELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(27224..28291)
                     /gene="rffG"
                     /locus_tag="ACHKXP_001408"
     CDS             complement(27224..28291)
                     /gene="rffG"
                     /locus_tag="ACHKXP_001408"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7646450.1"
                     /translation="MRKILITGGAGFIGSALVRYIINEMSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYSDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(28291..29553)
                     /gene="wecC"
                     /locus_tag="ACHKXP_001409"
     CDS             complement(28291..29553)
                     /gene="wecC"
                     /locus_tag="ACHKXP_001409"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO7646451.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(29550..30680)
                     /gene="wecB"
                     /locus_tag="ACHKXP_001410"
     CDS             complement(29550..30680)
                     /gene="wecB"
                     /locus_tag="ACHKXP_001410"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO7646452.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDKLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30736..31782)
                     /gene="wzzE"
                     /locus_tag="ACHKXP_001411"
     CDS             complement(30736..31782)
                     /gene="wzzE"
                     /locus_tag="ACHKXP_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO7646453.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31794..32897)
                     /gene="wecA"
                     /locus_tag="ACHKXP_001412"
     CDS             complement(31794..32897)
                     /gene="wecA"
                     /locus_tag="ACHKXP_001412"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO7646454.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(33137..34396)
                     /gene="rho"
                     /locus_tag="ACHKXP_001413"
     CDS             complement(33137..34396)
                     /gene="rho"
                     /locus_tag="ACHKXP_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO7646455.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(34481..34582)
                     /gene="rhoL"
                     /locus_tag="ACHKXP_001414"
     CDS             complement(34481..34582)
                     /gene="rhoL"
                     /locus_tag="ACHKXP_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO7646456.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34723..35052)
                     /gene="trxA"
                     /locus_tag="ACHKXP_001415"
     CDS             complement(34723..35052)
                     /gene="trxA"
                     /locus_tag="ACHKXP_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO7646457.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            35183..36448
                     /gene="rhlB"
                     /locus_tag="ACHKXP_001416"
     CDS             35183..36448
                     /gene="rhlB"
                     /locus_tag="ACHKXP_001416"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO7646458.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            36582..38066
                     /gene="gppA"
                     /locus_tag="ACHKXP_001417"
     CDS             36582..38066
                     /gene="gppA"
                     /locus_tag="ACHKXP_001417"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO7646459.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWSLEVH"
     gene            complement(38113..40134)
                     /gene="rep"
                     /locus_tag="ACHKXP_001418"
     CDS             complement(38113..40134)
                     /gene="rep"
                     /locus_tag="ACHKXP_001418"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO7646460.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            40351..40800
                     /locus_tag="ACHKXP_001419"
     CDS             40351..40800
                     /locus_tag="ACHKXP_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO7646461.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYNGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            40999..41280
                     /gene="ppiC"
                     /locus_tag="ACHKXP_001420"
     CDS             40999..41280
                     /gene="ppiC"
                     /locus_tag="ACHKXP_001420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO7646462.1"
                     /translation="MAKTSAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(41367..42842)
                     /gene="ilvC"
                     /locus_tag="ACHKXP_001421"
     CDS             complement(41367..42842)
                     /gene="ilvC"
                     /locus_tag="ACHKXP_001421"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO7646463.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     INLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNGQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            42992..43885
                     /gene="ilvY"
                     /locus_tag="ACHKXP_001422"
     CDS             42992..43885
                     /gene="ilvY"
                     /locus_tag="ACHKXP_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO7646464.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNHK"
     gene            complement(43937..45481)
                     /gene="ilvA"
                     /locus_tag="ACHKXP_001423"
     CDS             complement(43937..45481)
                     /gene="ilvA"
                     /locus_tag="ACHKXP_001423"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO7646465.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(45484..47334)
                     /gene="ilvD"
                     /locus_tag="ACHKXP_001424"
     CDS             complement(45484..47334)
                     /gene="ilvD"
                     /locus_tag="ACHKXP_001424"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO7646466.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGD"
     gene            complement(47399..48328)
                     /gene="ilvE"
                     /locus_tag="ACHKXP_001425"
     CDS             complement(47399..48328)
                     /gene="ilvE"
                     /locus_tag="ACHKXP_001425"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO7646467.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(48348..48611)
                     /gene="ilvM"
                     /locus_tag="ACHKXP_001426"
     CDS             complement(48348..48611)
                     /gene="ilvM"
                     /locus_tag="ACHKXP_001426"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO7646468.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(48608..50254)
                     /gene="ilvG"
                     /locus_tag="ACHKXP_001427"
     CDS             complement(48608..50254)
                     /gene="ilvG"
                     /locus_tag="ACHKXP_001427"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO7646469.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(50257..50307)
                     /gene="ilvX"
                     /locus_tag="ACHKXP_001428"
     CDS             complement(50257..50307)
                     /gene="ilvX"
                     /locus_tag="ACHKXP_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO7646470.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(50394..50492)
                     /gene="ilvL"
                     /locus_tag="ACHKXP_001429"
     CDS             complement(50394..50492)
                     /gene="ilvL"
                     /locus_tag="ACHKXP_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO7646471.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            50845..52365
                     /gene="yifB"
                     /locus_tag="ACHKXP_001430"
     CDS             50845..52365
                     /gene="yifB"
                     /locus_tag="ACHKXP_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO7646472.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(52390..52728)
                     /gene="maoP"
                     /locus_tag="ACHKXP_001431"
     CDS             complement(52390..52728)
                     /gene="maoP"
                     /locus_tag="ACHKXP_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO7646473.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            52847..53686
                     /gene="hdfR"
                     /locus_tag="ACHKXP_001432"
     CDS             52847..53686
                     /gene="hdfR"
                     /locus_tag="ACHKXP_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO7646474.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(53782..53857)
                     /locus_tag="ACHKXP_001433"
     tRNA            complement(53782..53857)
                     /locus_tag="ACHKXP_001433"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53822..53824),aa:Trp,seq:cca)
     gene            complement(53866..53942)
                     /locus_tag="ACHKXP_001434"
     tRNA            complement(53866..53942)
                     /locus_tag="ACHKXP_001434"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53906..53908),aa:Asp,seq:gtc)
     gene            complement(53997..54112)
                     /gene="rrf"
                     /locus_tag="ACHKXP_001435"
     rRNA            complement(53997..54112)
                     /gene="rrf"
                     /locus_tag="ACHKXP_001435"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        13220 a        14766 c        13788 g        12420 t
ORIGIN      
        1 gctatgcgcc gctcggtttg tacatcgaaa acggtcagca gaaggtggcg ttaaatctcg
       61 cttcaggtga agggaatttc tttatccgtc ctggcggcgt gttttatgtc gcgggagata
      121 aagtcggcat cgttcgtcta gatgctttta aagccagtaa agatattcag tttgcggtgc
      181 agtcagggcc aatgttgatg gaaaacggtg taattaatcc gcgtattcat cccaacgtcg
      241 cctcacgtaa aattcgtaac ggtgttggga ttaataaaca tgggaatgcc gtgtttttgt
      301 tgagccagca ggcgacaaat ttttatgatt ttgcctgtta tgccaaagcg aaactgaacg
      361 ttgagcagct gctttatctt gatggcacga tttcacatat gtatatgaaa ggcggggcaa
      421 tcccgtggca acgctatcct tttgtcacca tgatttccgt agagcggaaa ggttaaaacg
      481 atagttttgt gtcagatact gaagatggcg cagatgccgg atgcggcgta aacgccctat
      541 ccggcataca tgacgttgca atttattgaa tatgaatgat tttttaggcc aaataaggtg
      601 cgcagcaccg catccggcaa cgttacaccg actccagccg ggcgtatgcc gccaccagcc
      661 atttaatacc ctggccctga aatgccacct gcaaacggct atgctcaccg ctgccttcca
      721 tattgacaat ggtgccttca ccaaacttag cgtggcgtac gcgctggccg agcttgtagc
      781 cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg gctgaccggg cggcttaccg
      841 tggcgcgcag gcgcacctct tccacacact cttccggcag ctcgccgata aagcgcgacg
      901 ggcgatggta aacctcttta ccatacagac ggcgggtttc cgcgtaggtc agcgtcagtt
      961 tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg ttcttcttcc agacgcccgc
     1021 cttcatccag cgacatctgg cttgggaaca tgccctcttc cataccaacg ataaacacct
     1081 gcgggaactc caggcctttc gccgagtgta gcgtcatcaa ctgcaccgca tcctgccagg
     1141 tatccgcctg cccttcacct gcttccagtg ccgcatggga gaggaacgcc tgcagcggca
     1201 ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt tgccgtcacc agttcctcta
     1261 agttttcgat acgcgtctga cctttttcgc ccttctcctg ctcatacatg gtacgcaggc
     1321 cggagtcttt aattacccgg tcagtctgta catgcagcgg catatcggca gtttcctgcg
     1381 ctaaggcgtc gattaattcc ataaatcgct gcaaggcgct ggcagctcgc ccggcgaggg
     1441 ctttttcctg caacagctca cgacatgcct gccagagtgt taactggcga tcgcgcgatg
     1501 tctgacgtac cacgtccagc gtccggtcac caataccccg cgttggcgta ttcaccacac
     1561 gctcaaaggc cgcgtcgtcg ttgcggttgg caatcaggcg cagatacgag agcgcatctt
     1621 tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat acggtacggc atactggcct
     1681 gcaataacgc ctcttcgagc acccgcgact gggcgttgct gcggtagaga atggcgcact
     1741 cggcaagcgc tccgccgttg tcctgccagg ttttgatgcg gttaaccaca aaacgcgctt
     1801 catcgagttc gttaaaagcg caatagaggg aaataggctc accgtccgcg ccatcggtcc
     1861 acagtttttt acccagacgc ccgttattgt tttcaatcag ggcgttagcg gcgctcagaa
     1921 tattgctggt agagcggtag ttttgctcca gacgaatagt ttcggcaccg gggaaatcat
     1981 taaggaaacg ctgaatattc tccacctgcg ccccgcgcca gccgtagatt gactggtcgt
     2041 catcaccgac gatcatcact ttgccggtgt cgcccgccag caggcggatc cacgcgtact
     2101 gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt ggtaaaacgt tcgcggtagt
     2161 gttgcaggat atgcggcttg ttaagccaca actcgtgagc gcgcagcagc agctcggcga
     2221 agtccaccag gcccgcgcgg tcacacgctt cctgatacgc ctgatacacc ttctgccagg
     2281 tctgctccac cggattaccg tagctttgaa tatgatgcgg acgcaggcct tcatctttct
     2341 ggctgttgat gtaccacatt gcctgccgcg gcggccactg cttctcgtcg aggttcatgg
     2401 ctttgatcag acgcttaagc aggcgtagct ggtcttcact gtcgaggatc tggaaatcct
     2461 gcggcagatt ggcgtccata tggtgcgcac gcagcaaacg gtgcgccagc ccgtggaagg
     2521 tgccgaccca cataccgccc tggctcgtgc ccatcagttg cccgatacga tgacgcatct
     2581 ccgccgccgc tttgttggta aacgtcaccg ccataatcga gtatggcgag cagttttcca
     2641 cgctcatcaa ccaggcgata cgatgcacca gtacgcgcgt cttaccactg cccgcgcccg
     2701 ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc gcgctgtttg tcattaaggc
     2761 tgtcgagcag gtaagaaacg tccattggca ccgccgcgta aaaataggtt gggcggagaa
     2821 gcgcaacgcc ctccgccaaa aagctgggta tatatacaga tttgctgatt atatcagcga
     2881 ggtcagagat gccaaccggg aaatttccag atgcggcagt aaacggctgt cccaggtttg
     2941 catcagatcg ccattttccg gtctgatcca acaagcctgc attccgctgc gaattgcccc
     3001 acccacgtca gtggtgagat cgtccccaac atgtaagatc tcgccgatcg gcacgttgag
     3061 tttttccgca gccaaaaagt acatatcgct gaacggtttt gagcgcccgt gcgggccagc
     3121 gcgcagcaca aactcaaaat aatcccccaa accaaacagc tccggctggg cgttaccgtt
     3181 ggtgatcgcc accagcggcc atttcttcgc cagctgtttt aaggtgtcgt gagtttgctg
     3241 cgggacgtcg attcggctgc gccatttggc aaagttgatc attgctgcgt gtgcgcctgc
     3301 actggcttct tcggcactca gcccggcgtc gagcatcgct tgttcaatcg aacgaaaacg
     3361 ccagcgcgtc acgtcgtgat aaatctcggg ttccgcttcc cgtaccgcct ggcgcaggcg
     3421 ttgcagatct tcattctgga agctgcgcag cgccggatga taattttgca caaaggtaag
     3481 cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg taaagggtat catccaggtc
     3541 aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa cgcattattt cccccgtttg
     3601 gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt gaaaatcaag atgagtatag
     3661 atttgcgtgg tggagaggtt ggcatgaccc agcagctcct gcacaccacg aagatcgccg
     3721 ctcgactcca gcatatgcgt ggcgaacgag tgacgtaatt tatgcggatg aacgtgatta
     3781 ttcagccctt gttttatgcc ccattcggca aagcgtttct gcacattacg cgcggagata
     3841 cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc taccgaacag gtcgcgcaaa
     3901 tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa tcggcaggcg gcgctctttg
     3961 ctgcctttcc ccataaccca cacttcacca gactccaggt cgaggtgttt aatatccagc
     4021 cccaccagct cagaaagacg cagacccgcg ccgtacatca cttccagcat tgcacggtcg
     4081 cgtacagcga ggggatcatt gatatcaata tccagcagcc gattcatatc gtcgacgtcg
     4141 atgtttttcg gcagatgacg cggcgctttc ggtgccgaaa cacctttcgc cgggttagct
     4201 ttgagttcgt tctggctgac cagccagtca aaaaagctac gtagcgcaga aagccgtaac
     4261 gccagacttg ctgctcccag ccctttacgg cgactgcgta cagcaaaatt gcgcaccatc
     4321 gtcacatcac attgctgcca gctttgcagg ccgttttcgc tggcaaaatt gatgatcgcc
     4381 tcaagctgac gctggtagtt aagcagggtt atcgggctaa gctggcgctc cacgctcaga
     4441 taacgtaggt agcgttctac atcggtgtgt aaatcggtca tacgcgttca atccaacgct
     4501 ccagaagctc cggcaacatc agcgcaattt catgaagtaa ctgcgttcct tgcccttgtt
     4561 gatagtgact ggcatcgcga ctggtaaaca gcacgacacc caaatcagca tcgcttccca
     4621 gcatcgacat cgccaccgat cccaccgctt tcgcttccgg tagcaccacc agcagctctg
     4681 gtccgttaag cggcccaaga tagtgctgtt cctgccccaa acgctgaata cgcagcggtt
     4741 cgaaagactg acggcttaat gccagatgag tgtggttcga cggcgcacct aagcgccagc
     4801 gatccggaaa caggcgcaga ctcgcacctg ccaggccgag atcgcgcgcc cagcggtgaa
     4861 agcgcatcag catatcgtcg agactgctgg cggcggtgag actgcgctgc aggtagagta
     4921 ggcgataaaa caggccttcg ttggcgatag cctgttccat caacagcgcc atgttctctt
     4981 ccagaacatg aatatgatta cgtgcgcggg ccatgtgcca ctcgaccaac gaaacggtgc
     5041 cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg cgcattacgg ataaaaaact
     5101 caggattttt aatcagataa tcgacaaccg cccggtcatc aagctccgtg agtgtttcct
     5161 gcagttcttc ccctggttgc ttcatagatg aataaatccg tcgtagacat gtaccgccgg
     5221 gccagtcata tataacgggt gacccggacc tttccaggcg atatcaagac gaccgccggg
     5281 gagttccacg cgtacttctt cggccagcaa accttgctga atccctactg caaccgccgc
     5341 acacgcgccg ctgccgcagg cctgggtttc tcctgcccca cgctcataaa cgcgtaaacg
     5401 aatatgctcg cgcttaacca cttgcataaa accgatattg gcgcgctccg gaaaacgctc
     5461 gtggctttcc agaacaggac caagcgtttc taccgccgcg gtatcgacat catcgacctg
     5521 aatcacgcaa tgcggatttc ccatcgacac cacgccgcat aagattgtct gctcggcggc
     5581 gcgcataata taggtctttt ccgctttgtt agcgcgaaac ggcacggcgg aaggttcgaa
     5641 gttgggttcg cccatattta cgcggaccag atcatcatcg gtgacggtca gaaccatccg
     5701 cccgttggcg gtgctgacgc ggatatcacg cttattggtc agtcctttca gacgcacaaa
     5761 acgggcaaag cagcgcgcac cgttgccgca ctgcgccact tcactgccat cagcattgaa
     5821 aatgcgatag tgaaaatcca gttcaggatc atacggcggc tcaaccacca gcagttggtc
     5881 aaaccctacc cccaggtgcc gatcagccag gcgacgaatc agctccggtg aaaaaaagac
     5941 attctgcgtt accgcgtcga cgaccataaa atcgttgcca aggccatgca ttttcgagaa
     6001 ctgcatcatt tactccaatc acgcgggtac agaaactgac ttttagtaat tcacctggga
     6061 tggaccatcg ccagtggcgc gatcgttttt atccggcacc gtggattgcg tttgcgtctc
     6121 taccggtttg gtcggcggcg gtgcgttttt atctgcaggc gggaaataga gcggaccttt
     6181 cagaccgcag cccgtcaggc tgaagagagt aagtaataca gtgagtgcct taaacacgtt
     6241 tttcattatg gattgcctgt aaagttcatc gctttctgct ttctatcatc gcaggagaag
     6301 acgtaaaagc aagcgtttag cgactcttta tgaagtctga aaagtgagga aatgctgcga
     6361 gtataacgct tactcgtggg gatttatgtt ttgctggtag tttcgtgatc ggcacctcgc
     6421 gccaggatgg ctttatgcac tatcgcaatg tggtttgttt gttgtctttt ttagttccgt
     6481 cagcgtgagg atgctcgctc ggtaaacctg atcataatgt ttaccagaaa cagggaaaga
     6541 cgtggtgtaa ctgcgcccta atgaagagac aaatctttcg ctacacagag acatcattat
     6601 tccggtgacg gatgaatcgt gccggaacgc agccgcagag gtgcaaactt tgaagccctg
     6661 accccatgcc gctatactgc cgccatcact taaaaacagg atacaacaat gaacgacagt
     6721 gaatttcatc gcctggctga tcaactctgg ctgaccattg aagaacgcct ggacgactgg
     6781 gatggcgaca gcgatatcga ctgcgaaatc aacggcggcg tactgaccat tacctttgag
     6841 aatggcagca aaatcattat caaccgccag gagccgctgc accaggtatg gctggcaacc
     6901 aaacagggcg gctaccattt tgacctgaaa ggcgatgagt ggatttgtga tcgcagcggc
     6961 gaaaccttct gggatttgct ggaacaggcg gcgacgcagc aggcgggtga aacagtcagt
     7021 ttccgctaag attgcatgcc ggataagcct cgctttccgg cacgttcatc acgaaaaata
     7081 ttgctgtaat agcggcgtat cgtgatcctg attggcaggc ggcatgttac cgatagattt
     7141 tgtgcggaac ggaatcacct gttcacgacc atcaaccttc acaatctgat agaactgcgg
     7201 caggttgaag ttgatgaagc ttgagccgta ggtaaaacgg tcatgcgatg acgagtagaa
     7261 gcgactgacg tcacgtacca gctcctcttt gctgccttcg cagtggtgat atacctcaac
     7321 ccggttgctt tcgtcgagaa tgtagatatt aaagccattc tcgtcttgcg tttcttcgaa
     7381 aaagaactgg atgatccctt cgctggcaaa gccgtccacc accgccggta atttgacgtg
     7441 attggtttca acctgcactg acaggccgtg cagtttgtta tgcgaaatcg cgccataaaa
     7501 ctcgatggcg ttttccagtt tctgtaccga tacattcagg cgttcgaaga acaaccccca
     7561 ggtttgacca gaaacgcgca gcgccttgaa acgcccggtt tcctggcggg tgctggaaag
     7621 acgcaattca atacactcag aaaccagttg ctgcacgcga gtacgaatta agccgcgcag
     7681 atgctggcta taacagaaga cttccacgct atctggcggt gcggcgtcct gatgcatttt
     7741 gccgagaata gttttcaggg cttcgatcat cgattgctcg ccgttgaagt gcagcgtacg
     7801 cacttcgttc cacgagttgc ggtacagcag gtcaacgcta cctaccaggc aattttgatt
     7861 ctcgccaaag ctgaagacat ccagcttacg gaaatcgaaa tgcaccacct gattgcggaa
     7921 cgccgctgtc gggtcatatt ccaggttaac gataatcgcc agatggcgga tctcacacgg
     7981 gctgtagagc gccttcggtg tcggtgcagg taaacgcagc gggaaatggt gcgacacgtc
     8041 ggcgaccatc tcctgcaact taggcaaatc gacaatgccg ttacctttaa tatacaaacg
     8101 ggtgcgcgag gtcagcaggc cgttaaacca tgcccacgcc accagtttat tcaggtaacg
     8161 gttatattcc agcggctgat ggctgatgat cgactcaata tttggcgcgc ggttatacag
     8221 ataccaacct gaacggttag cccggcccgg cggcacataa ataaaggtca gattcggttc
     8281 cgagagatcg ggtgaaatct gcgggtttac cagcgtcact ttacctggta atgcttcaaa
     8341 cgcggcatac agcttacgcg tcagcacgcc gatatcctgc ggactggcgg agacgctaag
     8401 gttattgcga cgcgcaaagc ggatcagatt acggtagctc tgcatcatcg cgtcgagcaa
     8461 ctcgttgtgc gcctcacgca cctgatcaat cttccagtta gcgcggttat cgagcattgc
     8521 cagacgagct tcgtcccaac cccactcgct cactaactgg ctcaacactg cgcgacgcca
     8581 gcctacgcag gcgcgttcac ggctgagctt ttcgcacact tttaaataga agcagcgacg
     8641 tactaaatcc agacgggtaa aatcttcaat cgccgtcagg tattcagtaa cacgctccag
     8701 catcatgcag tatggatcga gaccaaacga tacaatctcg ccgtcgtgca aacgctgttt
     8761 gatatctttc gccagcagac gtgggttcgg gtattcccag gaataggctt ccagcagcag
     8821 tgttttcagt accgctttgt atggggaatc gatactcttg tagagctgcc aaaggctggc
     8881 accaaagtac tcttcagcag aaagcgagct taagccaccg agatccagcc attcatttgg
     8941 cgtcagcacg ccctgcgcgt aaagcgtcat cacatagtcg tcgtaatgct cttcttcgtc
     9001 gcacggcacc atattccaca gaatacgctt accggcgaga cgcacggcgg tacgataaaa
     9061 ttcgtcaagc agcagtatat gctgggtgga gccacaatct tcgcccccca ggctgccgct
     9121 ttcattatga cggaagcggt tttcatcaat caggaagaag ctgacttcca cacccagcga
     9181 ggcggcccag ttttccagca ggctacattt acgttgtagc aattggcgct cttcgctatc
     9241 gagccaggat tgatgacaga cccagatatc caggtcagag gaacaacttt gccctacgga
     9301 cgaggtgctg cccatggtgt atacaccagt aattggaagc tcacctttcg gcggatcctg
     9361 tactgacatt ccacgataca gttcaagctc gttcaggtag tggcgttgag tttcatcagg
     9421 cgtgtaaagg caaatgcctt tgggaacgtt accatcaagg taacccggca ttagcggatg
     9481 gtgatagtgc aacaatgtcg gcagtagact gtagacctgt tggaatgcag gccccatagc
     9541 agcaagcgcg cgatccacac gcaattgatt tatggcatcc agtctctgtt tcagagtctc
     9601 aatatagagg tacaagacgt atcgcctgat ttgctacccg tcatgactgt gattccgcca
     9661 acatcaacgg taacacgcgg cattcgggat atttcgtatg tcaaaggtaa ccgttaccac
     9721 ttttcgcgcc tggttttttt agtttcacga cgaaaaaatg gtctaaaacg tgatcaattt
     9781 aacaccttgc tgattgaccg taaagaaaga tgcgctacat acaagtgtag caccgtttat
     9841 tctctgtaaa ttccttatta caacggcgtg aaacgcctgt caggatccac tgccagacct
     9901 cattttacgg tttgcgcagg cgtctacgtt tcaccacaac actgacatca ctctggcaag
     9961 gatgttagga tggaccacgg atgataatga cggtaacaag catgttagac aatgttttaa
    10021 gaattgccac acgccaaagc ccacttgcac tctggcaggc acactatgtc aaagacaagt
    10081 tgatggcgag ccatccgggc ctggtcgttg aactggtacc gatggtgacg cgcggcgatg
    10141 tgattcttga tacgccgctg gcgaaagtag gcggaaaagg cttatttgta aaagagctgg
    10201 aagtcgcgct cctcgaaaat cgcgccgata tcgccgtaca ctcaatgaaa gatgtgccgg
    10261 ttgaattccc gcaaggtctg ggactggtca ctatttgtga gcgtgaagat cctcgcgatg
    10321 cctttgtgtc caataactat gacagtctgg atgcgttacc ggcaggcagt atcgtcggga
    10381 cgtccagttt acgtcgccag tgccaactgg ctgaacgccg tccggatctg attatccgct
    10441 ccctgcgcgg caacgtcggc actcgcctga gcaaactgga taacggcgaa tacgatgcca
    10501 tcattcttgc cgtagccgga ctaaaacgtt taggtctgga gtcacgtatt cgcgccgcgt
    10561 tgccacccga gatttctctt ccggcggtag gacaaggtgc ggtgggtatt gaatgccgcc
    10621 ttgatgattc acgcactcgc gagctgcttg ccgcgctgaa tcaccacgaa actgcactgc
    10681 gcgttaccgc agaacgcgcc atgaataccc gtctcgaagg cggatgtcag gtgccaattg
    10741 gtagctacgc cgagcttatt gatggcgaaa tctggctgcg tgcgctggtc ggcgcgccgg
    10801 acggttcgca gattattcgc ggtgaacgcc gcggtgcgcc gcaagatgcc gaacaaatgg
    10861 ggatttcgct ggcagaagag ctactgaata acggcgcgcg cgagatcctc gctgaagtct
    10921 ataacggaga cgccccggca tgagtatcct tgtcacccgc ccgtctcccg ctggagaaga
    10981 gttagtgagc cgtctgcgca cactggggca ggtggcctgg cattttccgc tgattgagtt
    11041 ttctccgggt caacaattac cgcaacttgc tgatcaactg gcagcgctgg gggagagcga
    11101 tctgttgttt gccctctcgc aacacgcggt tgcttttgcc caatcacagc tgcatcagca
    11161 agatcgtaaa tggccccgac tacctgatta tttcgccatt ggacgcacca ccgcactggc
    11221 actacatacc gtaagcggac agaagattct ctacccgcag gatcgggaaa tcagcgaagt
    11281 cttgctacaa ttacctgaat tacaaaatat tgcgggcaaa cgtgcgctga tattacgtgg
    11341 caatggtggt cgtgagctaa ttggggatac cctgacggcg cgcggtgctg aggtcacttt
    11401 ttgtgaatgt tatcaacgat gcgcaatcca ttacgatggt gcagaagaag cgatgcgctg
    11461 gcaatcccgc gaggtgacga cggtcgttgt taccagcggt gaaatgttgc agcaactctg
    11521 gtcgctgatc ccacaatggt atcgtgagca ctggttacta cactgtcgac tattggtcgt
    11581 cagtgagcgt ttggcgaaac tcgcccggga actgggctgg caagacatta aggtcgccga
    11641 taacgctgac aacgatgcgc ttttacgggc attacaataa ctctcataac aggaagccat
    11701 aatgacggaa caagaaaaaa cctccgccgt ggttgaagag accagggagg ccgtggacac
    11761 cacgtcacaa cctgtcgcaa cagaaaaaaa gagtaagaac aataccgcat tgattctcag
    11821 cgcggtggct atcgctattg ctctggcggc gggcgtcggt ttgtatggct ggggtaaaca
    11881 acaggccgtc aatcagaccg ccaccagcga tgccctggct aaccaactga cggcattgca
    11941 aaaagcccag gagagccaaa aagccgagct ggaaggcatt attaagcaac aagctgcaca
    12001 gcttaagcag gcgaatcgtc agcaagaaac gctggcaaaa cagttggatg aggtccaaca
    12061 aaaggtcgcc accatttccg gcagcgatgc taaaacctgg ctgctggctc aggccgattt
    12121 tctggtgaaa ctcgccggac ggaagctgtg gagcgatcag gacgtcacga ccgctgcagc
    12181 gttgctgaaa agtgcagacg ccagcctggc ggatatgaat gacccgagtc tgattaccgt
    12241 tcgtcgggca attaccgatg atatcgccag cctttctgca gtatcgcagg tggattatga
    12301 cggcatcatc cttaagctta atcagctttc aaatcaggta gataacctgc gtctggccga
    12361 taatgacagc gatggttcgc cgatggattc agacggtgaa gagctttcca gttccatcag
    12421 cgaatggcgt atcaatctgc aaaaaagctg gcagaacttt atggacaact tcattacgat
    12481 tcgccgtcgt gatgacaccg ccgtaccgct gttagcgcca aatcaggata tctatctgcg
    12541 cgaaaatatt cgctctcgcc tgctggtcgc agcacaagct gtaccgcgtc accaggaaga
    12601 gacttatcgc caggcgctgg agaacgtctc cacctgggta cgtgcttact acgatactga
    12661 tgatgccacc accaaagcgt tcctcgacga ggtggaccag ttaagccagc aaaatatctc
    12721 gatggatctt ccggaaaccc tgcaaagcca ggcgatgctg gaaaaactga tgcagactcg
    12781 cgtgcgtaac ctgctggcac aaccggcagc agggacaacg gaagctaaac ctgcacctgc
    12841 acctgcacct gcacctgcac ctgcacctgc acctgcaccg caaggtgata ctccggcagc
    12901 cgcgccgcaa ggagaataac catgctaaaa gtgttattac tctttgtgtt gctgattgcg
    12961 gggatagtgg ttggcccgat gattgccggt catcagggtt atgtgctgat ccagaccgac
    13021 aactacaata tcgaaaccag cgtcacgggc ctggcgatca tattgattct ggcgatggta
    13081 gtgctgtttg ccattgagtg gctactgcgg cggatcttcc gcactggcgc gcacacccgt
    13141 gggtggtttg tcgggcgtaa gcgtcgccgt gcacgtaagc agaccgaaca ggcgctgctg
    13201 aaactggcgg aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac
    13261 gctgaacaac cggtggtgaa ctatctactg gctgccgaag ccgcgcaaca acgtggtgat
    13321 gaagcgcgcg ccaaccaaca tctggaacgc gcagcggagc tggccggcaa tgacaccatt
    13381 ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc
    13441 cacggcgtgg ataagctgct ggaagttacg ccacgccatc cggaagtatt acgtctggcg
    13501 gaacaggcgt atatccgcac aggtgcatgg agttctctgc tggatatcat cccatcaatg
    13561 gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt
    13621 ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag gtttgcgtaa ctggtggaaa
    13681 aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg tggcaatggc ggaacatctt
    13741 attgaatgtg acgatcatga tactgcccag caaattatca tcgatggcct gaaacgccag
    13801 tacgacgatc gcctactgct gccgattcct cgactgaaaa ccaacaatcc ggaacagctg
    13861 gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca
    13921 ctgggccagt cactgatgaa gcacggagaa tggcaggaag catcgctcgc cttccgcgca
    13981 gcgctgaaac aacgtccgga cgcctacgat tacgcatggc ttgccgacgc gctggacaga
    14041 ctgcacaagc cggaagaagc tgcagcgatg cgtcacgacg gtttgatgtt aacgttgcag
    14101 aacaacccac cacagtagtt ccttctcacc cgggggcaag cacctccggg gccttcttga
    14161 cacataaaaa aacgcctgct cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa
    14221 caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg atgaggcata agcgacatct
    14281 gtcagtggac gataagcacc gtaaacggct ctgcgtcatt ccggagttta tgaggcacta
    14341 aggcgaacat aagagatgga atgagcatct actcgtttat tatgccacag agaatcggga
    14401 aataacatcc cttaacactt gttatgagat aattctgtaa tcctctttgc ttcctgagta
    14461 ataacttcct gagtgaatat ttaacctgag cttgatccta cacatactta ttatgaatga
    14521 taaaattcat tcaattaata acacatatat taattgccgt taaaactaaa aacagcatca
    14581 ataatcaacg cgatataata aacctgcctt acatatcaac tgcgccagag gtaggattga
    14641 aaacgctctc ctgattttcc aattcatttt ctggataaat aaataattta tttttgtcac
    14701 tattatttat gtaatcatcc tgtcagggag agggatctca attatcaatg cttaattacg
    14761 tcatcatttt gatgacatga aaccggagca ttttgcatgg aaatttcgtt ttcacccaaa
    14821 cgtcttgttg ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc agataccccg
    14881 tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc acccaaacca gtatctggtt
    14941 aaaccggcga ccactattgc cgacaacatg atgccagtaa tgcagcatcc ggcgcaggat
    15001 aaagaaaccc agcagaagct ggcagaactt gagaaaaaaa ccggtaagaa gcccaatgtg
    15061 gttatcttcc tgctggacga tgtgggctgg atggacgtcg gttttaacgg tggcggcgtg
    15121 gcggtgggta accctacacc agatatcgat gcagttgcca gccaggggct gattttaacg
    15181 tcggcgtatt ctcaaccaag ctcttcccca acccgcgcca ccattctcac cggacaatac
    15241 tccatccacc acggcattct gatgccgcca atgtacgggc aaccgggagg tctgcaaggg
    15301 ttaaccaccc tgccgcaatt gctgcacgac caaggttacg tcacccaggc tatcgggaaa
    15361 tggcatatgg gggaaaacaa agagtcgcag ccgcagaacg ttggctttga tgatttccgt
    15421 ggctttaact cggtgtctga tatgtacacc gaatggcgcg acgttcacgt caatccggaa
    15481 gtggccctga gtccggaccg ttctgaatac atcaagcaat taccgttcag caaagatgat
    15541 gttcatgcag tgcgcggcgg cgaacaacag gccattgccg acattacgcc gaaatatatg
    15601 gaagatctgg atcaacgctg gatggaatat ggcgttaagt tcctcgacaa gatggcgaag
    15661 agcgataagc ctttcttcct ctattacggc actcgcggct gtcacttcga taactacccg
    15721 aacgctaaat atgcgggtag ctctccggca cgcacctcgt atggcgactg catggtggag
    15781 atgaacgatg tgttcgctaa tctgtataaa acactggaga aaaacggtca gcttgataac
    15841 acgctgatcg tctttacctc cgataacgga ccggaagccg aagtaccgcc gcacggacgc
    15901 accccgttcc gtggtgcgaa aggttcgacc tgggaaggcg gcgttcgcgt accgactttc
    15961 gtttactgga aagggatgat ccaaccgcgt aaatctgacg gtattgtcga tctggcagat
    16021 ctcttcccta ccgcgctgga tctggcaggg catcctggag cgaaagtggc gaatttagtg
    16081 ccgaaaacca cctttatcga tggtgtggac cagacatcct tcttcctggg aacaaatggt
    16141 cagtctaacc gtaaggccga gcactacttc ctcaacggta aactcgctgc tgtgcgtatg
    16201 gatgagttca agtatcacgt cctgattcag caaccttacg cttataccca gagcggatat
    16261 cagggtggat tcaccggcac agtaatgcaa acggcgggat cgtcggtgtt taacctctac
    16321 accgatccgc aggaaagcga ctccatcggc gtgcgccata ttccgatggg tgtaccgcta
    16381 cagaccgaaa tgcacgcgta tatggagatc ctgaaaaaat atccaccacg cgcgcagatt
    16441 aaatctgact aagccggcgc tatcgctgag atctgccttt gccggatgcg atgctgacgc
    16501 atcttatcca gcctacagaa cgctgcaatt tattgaattt gcacgatcat gtaggccgga
    16561 taaggcgttt acgccgcatc cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt
    16621 tacttactca ccaccagcaa atgcgcatgc ataatgtcgc tggccgggcg gccatgcgcc
    16681 agcaaatcag acattgcttt aagatacggc ggcagatggc gaaaataacg ctgatacccg
    16741 gcacataaat aattcagccc aggcttgccg ctggcatcga gcataaagcg gtgtttcggg
    16801 cagcctcccc agcacgcttt taacacgtta caactacgac actgcgccgg taactgctta
    16861 aatttatctt caccaaacac ctgctgttgc ggggaatcga tcatttctgc aattgtttgc
    16921 tggtgcatat tccccagccg atattgcgga taaacatagt gatcgcaggc gtaaacgtcg
    16981 ccattgtgct caacaatcac cgagcgccca caggttggct gatgatggca aaccgcaccc
    17041 ggcgcaccga caaaattggc aaacgcccat tcgatattca tcacgaaaat cttgccgacg
    17101 tcgcgtttga tccagtggtc aaaaatcgcc accagaaatt caccaaattc atcggggcgc
    17161 acagaccatt ccgttagctc accctgaata tcaccaggcg catgcaattt cagtccttcg
    17221 cgggcagccg tttcatcagc caggcgctcg accaccggaa taaactggat gaattccact
    17281 cccgcatcgc acaaaaaatc atatacctgc aacggttgct gcgcgctggt gcgattaacg
    17341 cagaccagca cgttatagtc gacatgatgt ttttgcagga acgtcagggc acgcatcacc
    17401 agcttatgcg tgggtctgcc acctttagtc acgcgatatt gattgtggat ctcaggcggg
    17461 ccatccagcg ataaaccaac aagaaaatga tgctccgcga gaaacgcgca ccattcgtca
    17521 tccagcagca cgccgttagt ctggaagctg ttacttatct tcctgccagc accatatttc
    17581 gcctgtagcg ctacggcacg gcggtaaaac tccagcccga gtagcgttgg ttcgccgccc
    17641 tgccaggtaa aagccacttc gttttgcggt tcgctggcag cgatatagtg acggacatac
    17701 gcttccagcg tgtcatcgtc catatgcgtg actggctttt cgcggtaaag ggattgtttt
    17761 tcgagataaa aacagtagtc acagttcaga ttacaatcgg aaccactcgg tttcgccatc
    17821 acatgaaaag cacgcgttgg aacctgttgc agcatggtca ctccttgcct gaagtcatcc
    17881 ttcaatacgt taagggcgaa atcataccgt ggggcaatga gttattacgg gcgtttattg
    17941 cgaagggagg gtgacgaaat gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa
    18001 ttggtcggcg agagaggatt cgaacctccg acccactggt cccaaaccag ttgcgctacc
    18061 aagctgcgct actcgccgaa atactgcttt ttgaattttt agttcaattc tttaaagtcg
    18121 tggtgcgagg ggggggactt gaacccccac gtccgtaagg acactaacac ctgaagctag
    18181 cgcgtctacc aattccgcca ccttcgcatt gtcacaactt ctaataatgg ggtggctaat
    18241 gggattcgaa cccacgacaa ctggaatcac aatccagggc tctaccaact gagctatagc
    18301 caccactaca aatcttgtta cgcggtatta ctaccaccgc agctcaagcg ccgggactaa
    18361 atggcgcgcc cgacaggatt cgaacctgag acctctgcct ccggagggca gcgctctatc
    18421 cagctgagct acgggcgctt agcgccgttg cgggggtgga taatacggac ttcccacccc
    18481 tctgtctagt ccctttttaa ataaattgcg cgtttggtta cggtttgtgc gttttgctgc
    18541 ttattcctcc agtttatgcg ctttcccgtg gcgattaagg ccaaaaactt tataaatcgc
    18601 cgtcaccgcc agcataaaga tgatgccaac aaacagcgac atacgcgtat cttcattaaa
    18661 gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt acgtaattgg cccacgggaa
    18721 caggattgag cggaacggat ggctggcaat cgccgcttta tgtgcacgcc gaaaacgcag
    18781 ctggcttatc aatatcacaa accatggcac catccccgga agcacgctgg cactgtagac
    18841 gtagacaaac acacgctgcg gattgggaat gatgtagttc aggcatgagc caattagcag
    18901 aatagcaata gatactgcca cacccgcaac cggtacgccg tgacgggaaa ctttcgccat
    18961 tgccgccggt aactgacggt ttttcgccag tgcgtagagc atacgtccgc aactgtacat
    19021 gccgctgtta cagccagaga gcgcagccgt cagcaccaca aagttgataa tgcccgccgc
    19081 tgcggtaata ccgattttgg caaaagtcag tacgaacggg ctgccgttgc tgcctatttc
    19141 attccacggg aagatggtga cgataacgaa aatcgcgcct acgtagaaaa tcaggatccg
    19201 ccacagcacc ttgcctacgg cactgcgcag cgtcacctgc ggattcttcg cttcaccggc
    19261 agtaatgcca atcagctcca cgccctggta ggacgccacc acaatacaca gagcggtcag
    19321 gaaccctttc cagccacccg caaagaaacc gccatgctct gtgagattgc taaaaccaat
    19381 cgactgcccg ccattgccaa agccaaagaa aatcacgccc aggccaatga caatcatcac
    19441 gataatcgtg gtgactttga tcatcgcgaa ccagaactcg atttcgccgt acaaccgcac
    19501 cgccgccaga ttcgccaacg ccaccagcgc cactgcgatc aatgcgggta tccactgcgc
    19561 catctccggg aaccagaact ggacataaac gccaatggcg gtgatttcag agatccccac
    19621 cgccatccac ataaaccagt aagaccaggc ggtgagatag ccaaagaacg ggctcatata
    19681 acgatgcgca taaacggcga acgaaccggt aaccggttcg aggaacaaca tttcgcccat
    19741 tgaacgcatg atgaaaaaga cgaacagccc ggcgatgata taggccaaca atacggatgg
    19801 cccggcccat ttcagggtac tggcggcccc cataaacagg ccgacgccaa tggtgccccc
    19861 cagggcgatg agttcgatat gtcgagcttc cagcccacgc tgtagctctg gtttgttatc
    19921 tgccataaat cctcgtgttg tgtttgcatg ctttccggtg ttaccggtta tcgttatggg
    19981 tacatcgagt gttgcaaatg ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa
    20041 atttgaatgc tttgtgtaat aaaaaagcag acaggcgacg gagtgaccac tccgtcgctt
    20101 tacaaagaga ggaaaatcat aggttgccgg tgtagtgcca gcgtaaataa cgcagcaaac
    20161 gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg gtagagccac tccagcccca
    20221 gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt gaaaacatcg taagtcccgc
    20281 caacgcccat atacagcgca tctggatgta ccagacggca gtcgcgcatg atgatctcct
    20341 gctttggcga tcccatcgca acggtgacga tttgcgcacc gctggcatga atgcgttcaa
    20401 acagcgcctg acgctgctcg ggtttaaaat aaccatcctg actgccaacg atattcacat
    20461 tccactggtt gcgcagttta gcttcagttt gcgccagcac ttcaggttta ccgcccacaa
    20521 gaaataccgg cgtcccttct ttgcctgcgc gcgccatcag ctcttcccag agatcggcac
    20581 cggcaacgcg ggaaacctgc gcctgcgggt actttttacg tactgaacgt acaacgctga
    20641 tgccatccgc atatttaaat tcggcagcgt taattaactc cctgacctcg gcgttatctt
    20701 caatagtcag cattttttca gcattaatgg caaccagcgt tccctgctta agctgcccgt
    20761 cagcaaacag ataatcgagg gcgtgctgca tatcacgcca accaatcaac tgtaagccac
    20821 gcagcgtata ggttggtgcc gtggtgttgt tattcattgt tatccttcaa cctgcgtccg
    20881 gagcgatgat tttgtacgtt tatgaatgag tccggcgctt tcaaaaagcc agtacaacag
    20941 ttttgcgatc atcagacatg cgccgaagac cacgataaaa aagaccacgc gtgagacaaa
    21001 cgaatccagc ccttcacgcg ccagcacgat catattgaag atcgccccaa agcagaaact
    21061 gtgcaatatc gcagctttat agcgattagg ctcgcggttg cccagctcat acagccagtc
    21121 gaaccatttg atgatcagac caaccacgat cgccccgagc gggatgaaca acgcgccgcc
    21181 catcaccacc agtgagccta taagcgtagg cgagatcgcc agtccggagt ggttattcag
    21241 cacttcccag gtaaagtagt tggctgagtt cagcaccata ctcgggcgac ccggccacag
    21301 ccaggaaggg ataaagacat agaaatcgcg gacaattgga gccaggccct ggaagtcgat
    21361 gttgtcgtag ttctgcaaca gcaacgccag attctcccac ggcgagaagg tgtcgcgagt
    21421 gagatagaga aacgtataga acgcttcatc gccgctcaca ttcattccat agcgttttag
    21481 tgccagccag aacatgccaa caatccccag cacgcccgcc gccgccagca tccacaacga
    21541 aatccagccg cgaataatgc caataaacag gaagatagcg aatgcgatga tgatattggc
    21601 gcgagtgccg ccgacaatca tataagtcag caagccaaag gcgaccgtgc tgacgaggaa
    21661 aaacagccac gctttgctgt cctggcgcag aaagtagacc accagcatcg ccgggatgaa
    21721 aaagtaaaag aagcgtttta acgccacgcc ggagacttca ctggaaaaga tctgactgta
    21781 ggagttaagc cggaacagca aaaagccgtt gtgcatgaag aagatgccga cgcttaccag
    21841 cgcgataccc atcaggatca cccacgtaag attggtctcc acgcggttca tggtaaacag
    21901 cggacggcgc ggtacatcag caacgcgttt gcgtaggcgg gttttgtagg tgacatagta
    21961 aaccgcgtag aagcagcccg cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac
    22021 accaacatca aagcgaaata ccagcacgct ggtcagcggg aagccgaaga aaaaggtgag
    22081 caaaaacagc aatgaaaaga agacattgaa gttaaagcgc acacggcgaa actcaaacca
    22141 ggtcagcgtg gcaataaaca gcgtgcagag cagccagaca acaaacaggc cactgaattg
    22201 cagcagactc atgcgacctc cccggcggca atcgccaacg cccgctgcca gccttgtaga
    22261 tagttagggc taaagaaggc aatggtgttt ttatccaccg acgccaactg gcgctgcgct
    22321 tcacgcacaa tatcctcgtt gagatcgtcg gtagtaaaca gcaccggcaa atgttgttcc
    22381 gtcatatcct gccagaacgg attttcccgg ttaagcacac aaggaatgcc cgcctgaatc
    22441 agtaagcaca gcgtaccaat gccctgctgg cgggcaaaaa taaagtaacc aagatcgcac
    22501 tgacgaagta gcgccagata ggcgtcaaat tccagttttt cgctcagaat ttgtagattt
    22561 tcttcgctga ataactccag ccccgcctga cgaacttcct caatgtacgc ttcgttatta
    22621 ggcggatatc ccatcggcac caccactttt accgtatcgc caaattgctg atgaacggcg
    22681 cgcaaggcag caatatgctc attgctgcgg tcgccggagt tccccaccag aatggtcatt
    22741 ttcccttcac gttgccgatc gttcgccatc gtattgagcg aagggtccat ccgcgtcggg
    22801 aagtacagca gttcgccccg cacctttggg tgcgttttgg caaaaaagct caaatcgccg
    22861 cgggtggcaa atacacagcc gactcgcttt tgcgccaggc gacgtagtgg gtaaaaaagc
    22921 ttatatctca agccactgga aagctcatac aggtctgccc cccagatatg ccagaaaaac
    22981 tggctgggct taatgccacc actcagcaga gccagccaca gtgtgggatt gaactgaccg
    23041 tggaagaaaa aacgctgctg acggttagct tttgctttcg cgatgaccgc ttccgccagc
    23101 gattttttcc cagggaaaaa ttgcacagaa agcgccggac agctatcact taagccgtcg
    23161 tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg tcgcggccag cgcgtcattg
    23221 aaaaaccgca aaacggttcg gttatggtga gggatatccg atcccagtac gtgaatcagt
    23281 acagtcatgc ccgcctacgc cagagtaaaa acacgccaca acaaagagaa aaatagacga
    23341 tataagttgc catatatgcc tgcgccgcgc ccagtgcacc atgcgcaggg attagccagt
    23401 gggcaaatac catcaataaa gtgaactggc tgacttccgc cagaatataa aaccgcagtg
    23461 acgctttggc gatcaccaga taaccaaaga cataagcgcc cacttttaac acatcaccca
    23521 ctaactgcca ggcaaagaga tcgcgcatag cggtaaattt attcgacaac agcagccaga
    23581 tagcaaaatc acgcagcagc cagacggtaa aactcgccgc cgccactgcc ggtaagacga
    23641 atttcagcga tttaaccact tcccgggtga tatcgcgctt ttccgttagc cgcgacaacg
    23701 tgggcagcaa atatacgctg aacgatgccg taataaattg caggtaggca tcggaaatac
    23761 tgctcacccc ttgccagatc cccacctcat cccagctata ctgcgccgcc agcagtttac
    23821 gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt
    23881 tgctcaactg ccctgccaga ccgttatccc agctgggttt cagatagctt aacgggatga
    23941 caccacgttt gatcaacatg atggcggcag gaattaccac cagcgcggga atcagcgcca
    24001 gacccagcaa cgccccttca taaccgccca aacggtaact gacgtagtac gcgagaacgc
    24061 caatcaagct gccgacaatc agcgataacg cattacctgc ggcatcgcga aagcctttca
    24121 tcagcgccag taacaggttg ccccaggcga tccccatttg caccagcgcc actaaacgca
    24181 ccagcccctg atagtcggta ttaccaaaca atccctggct gattggcgca gctgccagca
    24241 caaaaaccag cgccatcagc gtagagaaac caagtaccat cgctgatgaa gtgccgacca
    24301 cgcggcgcag ctgttgcgga ttatcatggt actgggcaac gtatttggtt acaccgttaa
    24361 agatgccagc cccggcaagc acgccgagca cggtaatcaa ctggcggaaa tttgccgcca
    24421 gcccaagccc cgccggacca aatgacaccg ccagcaactt accgaccagt aacccggcac
    24481 caatcttgac cagtgtactg gccgccgtcc acaaggacgc ttttgccaac gacatatcag
    24541 gaaaagtagt tcaacaaagt cgcaattacc gtacgctgat tgacgggcga caggttgtag
    24601 aacagcggca ggcgcagcag gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg
    24661 aactcaccaa agtgttcccc cgcagggcaa ccgtgcagcg gaatgtaatg aaacaccgcc
    24721 atgatttccg cttctttcag aaagttaatc aacgcgctcc ggtcatcaat atcccgcagt
    24781 ttaatgtaga acatatgcgc gttctgcacg cagccatcgg gaatcgacgg cagctcgata
    24841 cgcccggctt tcgccagagg cgctaacgca tcgtagtagt tttgccacag cgccagacgt
    24901 tgctggttga tacgatccgc tgcttccagt tgcgcccaca ggtatgcagc ttgcagatcg
    24961 gacatcaaat agctggagcc aatatcgcgc caggtatatt tatcgacctg accacggaag
    25021 aactggctgc ggttagtgcc cttttcacgg atgatctcgg ctcgttcgat taacgcttta
    25081 tcgttaatca gcgtcgcgcc gccttcaccg cccgccgtgt agtttttggt ttcatggaag
    25141 ctaaagcagc caatatgacc aatggttccc agtgcacgcc ctttgtaagt ggacatcacg
    25201 ccctgagcgg catcttctac cacaaacaaa ttatgctttt tcgccaacgc cataatggtg
    25261 tccatttcgc aggccacacc cgcgtaatgg accggcacga taacgcgcgt tttatcggtg
    25321 atcgccgctt caatcagcgt ttcgtcgatg ttcatggtgt ccgggcgaac atccacaaaa
    25381 acgatttttg cgccacgcag cacaaaggca ttggcggtgg agacaaaggt gtagctcggc
    25441 atgatcactt catcgccagg ctggatatcg agcagcagcg ccgccatctc cagcgaagcg
    25501 gtgcaggacg gcgtcagtaa cactttggcg ctgccaaaac gttgctccag ccactgctgg
    25561 cagcgacggg taaaaccgcc atcgccacac agtttgccgc tacccattgc cgactgcata
    25621 tagtcgagtt cggttcccac caccggcggt gcgttaaatg gaatcatgtg atcacctgta
    25681 taaccagtac gcggtgcttt ctacattcgc accactttgt atgtatcgtt taagcgcggc
    25741 ggtgttgccc atttgggtcg ccacccgcaa agttgtttta ccgcgagcat acgcccagtt
    25801 tagcgccgtt tgcatcagct cagcacctgc accgcgtcca gccagcaggc caattcgcgc
    25861 atctgtcgca ttgagttccc gtaaagagac atagccgcga atatcgccgg acgccgcacg
    25921 taaaatcaga cattgatgat caaaggtgcc gcgcacggca ttttcaatcc actgtgcata
    25981 aaagcgactg ctggcgtcag gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc
    26041 ggcgctggct aactgacgta atgcgggaat atcggtctct tgtgccacta cagcaccgct
    26101 atcactggca ttgttcacgg gtagcgccaa atcaacttca ccttctacca gggagaatcc
    26161 cagctgttgc agggcatcca gttcacccgt atttgatgcc gcaattttgg cctgcacccg
    26221 tgaccacggc gctaacgcgt ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat
    26281 ggcgctgtta acaccaaaga aggcgttttc ccaggttagt ggctcaatac tggcgcggac
    26341 gggcacgaag taactccagc agatattggc cgtagccagt tttcgctaat gaactggcag
    26401 cacgcttcac accctcgtca tcgagccagc cgttacgcca ggcaatctct tccaggcagg
    26461 caatcttaaa gccctggcgt ttttccaccg tctgtacaaa ggtgctggct tcaatcaggc
    26521 tgtcgtgagt gccggtatcc agccaggcaa atccgcgccc gagcagttca acggtcaggt
    26581 tgcccgcctc gaggtacatc tggttgatgg aggtaatctc cagttcatca cgctccgacg
    26641 gcttcacctg ctttgcgtac tccacgactt tactgtcgta gaaataaagc ccggtcaccg
    26701 cccagtttga cttcggctgt tttggctttt cttccagcga gatagcgcgg aaattgtcgt
    26761 caaactccac cacgccaaag cgttccgggt ccatcacctg atagccaaaa accgtcgccc
    26821 cttcggtgcg cgccgcaaca tgacgcagct tcggactgaa gccctgaccg aagaagatgt
    26881 tatcgcccag caccagacaa gaaggttcac cattaaggaa ggtttcaccg atgataaagg
    26941 cctgcgccag accgtccggg ctgggctgtt cggcatattc cagctggata ccgaactcac
    27001 taccatcgcc cagcaggcgc tggaaataac ctttatcttc cggcgtagtg atgatgagaa
    27061 tttcgcggat accggccagc atcagcaccg acagcggata gtaaatcatt ggcttatcgt
    27121 aaatcggcaa cagttgcttc gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac
    27181 cgcccgccag gataatacct ttcatgtttg cctccgctga aaattagcct ttcagaccta
    27241 aacgctcgcc ctgatagctg ccgtcctgca cctgcttcca ccagctttca ttagccagat
    27301 accactgcac cgttttacgc attccacttt caaaggtttc ctgcggcagc cagccaagtt
    27361 cacgggcaat tttcgaagca tcaatggcat aacgcagatc atgccccgga cggtcagcga
    27421 caaaggtgat caagtcacta tagtgcacca cgccgtgcgg cttgttcgga gccagttctt
    27481 ccagcagctc gcaaatggtt tccacaacat cgagattctt acgctcgttg tgaccaccaa
    27541 tattataggt ttcaccgact ttcccggtgg tcgccacgca atacagcgcg cgagcgtgat
    27601 cttccacata cagccagtca cggatttgct gcccgttgcc atataccggc agcgatttac
    27661 ccgccagcgc gttgaggatc atcagcggga tcagtttttc cggaaagtgg taaggaccgt
    27721 agttattcga gcagttggtg ataagcgtcg gcagaccgta ggtccgcagc caggcgcgca
    27781 ccagatggtc gctgctggct tttgacgcgg aataagggct gctcggcgca tacggcgtgg
    27841 tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata tacttcgtcg gtggagatat
    27901 gatgaaaacg gaacgctgat tttttatctt ccgtcagcgt attccagtaa gcccgcgccg
    27961 cttcaagcaa tgtataagtc ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa
    28021 tagaacggtc aacatggctt tcggctgcca gatgcatgac acagtctggc tgatgctcag
    28081 tgaatacgcg tgccagttct gcccgatcgc agatatcaac tttctcaaag gcaaagtgct
    28141 cgctttgcgc gaccggtgcc agcgacatca ggtttccggc gtaggtcagc ttatcgacca
    28201 ctaccaccgc atcgctcatt tcgttgatga tataacgcac cagcgccgag ccaataaacc
    28261 cggcaccacc tgttatcaga atttttctca tcagcgccag actcctttgg catcgacgac
    28321 atactgctga tggacattgt cgccattgat aactttgaac tgactatgat cgaccagcat
    28381 caccagcacg tctgccgttg ccagcgcctc gtcaagctgc gccagagtac aaagcccggt
    28441 cagttttttc ggcaactggt ggatgttagg ctcaacaacc agagtttcgc cgctatgcca
    28501 ctgggcgatc agttcagcga tttccatcgc cgggctttcg cgcaggtcat caatattcgg
    28561 tttaaacgcc agaccaaagc aggcgatttt cagttcactg gcgcgtttat cggtagccgc
    28621 caggcaatca gccaccgccg ctttcacctg atcgataacc cagaacggtt tgtgatcgtt
    28681 cacttcgcgc gcggtacgga taagccgcgc ctgctggggg ttctgtgcca cgataaacca
    28741 cggatcaaca gcaatgcagt gaccgcccac gccagggcca ggctgaagaa tattaacgcg
    28801 agggtgacga ttcgccaggc gaatcagttc ccagacgtta atcccctgat cggcacaaat
    28861 cagcgacaat tcattagcaa aagcgatatt cacatcgcgg aagctgtttt cggtgagctt
    28921 acacatttcc gccgtccgcg agttagtgac gacacactca ccttcgagga aaattttgta
    28981 cagttcgctg gcgcgggccg aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt
    29041 aatcagctcg accattacct gtcctggtaa cacgcgttcc gggcagtaag caatgttgac
    29101 gtccgcctgc tcgcccacct gctgcgggaa agtgagatcc ggacgcatct ctgctaacca
    29161 ttctgccatc ttctcggttg accccaccgg cgaggtggat tcaaggatca ccagcgcgcc
    29221 ttttttcagc actggcgcaa tggagcgagc agccgattca acgtaggtca tatctggctc
    29281 atgatcgccc ttaaacggcg tgggtacagc aatcagccag gcatccgctt caactggcgt
    29341 cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt actacactcg ccaaatcagg
    29401 ttcgacgata tggatttcgc cacgattgat ggtatcaacc gcatgttggt tgatatcgac
    29461 accaattacc tgtttttgcc gtgaggcaaa cgctgctgcc gttggcagcc cgatataacc
    29521 cagtccgata acagaaatgg tcgcaaaact catagtgata tccgattatt ttttaacgct
    29581 tccagaatgc gagagcatgc ctgaccatca ccatacgggt tatgggcgcg gctcatagct
    29641 tgatattcgt tttcgtcttt taaaagacgc gtcacttcct cgacaattcg ctgcttatcc
    29701 gtgcctacca gacgcaccgt acccgccgtc accgcttccg gacgctcagt ggtatcgcgc
    29761 atcaccagca caggtttccc cagcgaaggc gcttcttcct gaatgccgcc tgagtcggtc
    29821 aaaatcagcc aggcgtggtt catcagccag acaaacggta aatactcctg gggatcgatc
    29881 agaatgacat ttttcacatg ccccagaatg cgattgaccg gctctctgac gttcgggttg
    29941 agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgcg
    30001 tggcagattt cttcaaagcc acgaccgaaa ctctcacgcc tgtgaccggt caccagaatc
    30061 atctttttat cggggtcgat aaacgggtaa tttgccgcca gttctgaacg cagcttgtcg
    30121 ctgctcatca cctggtcacg cacccataac agtgcatcaa tgactgtatt accggtaatg
    30181 aagattcggc tatccgcaac gttttcacgc agcaagtttt gccgggaagt ttcggttgga
    30241 gagaagtgat acatcgccag atgcccggtc aatgtacggt tagcctcttc cggccacggc
    30301 gaatagagat cgcccgtgcg cagaccagcc tcaacgtgac caacaggaat acgctgataa
    30361 aacgccgcca ggctggttgc cagcgtcgtc gtcgtatcgc cgtgaaccag cacgacgtct
    30421 ggtttgaact cggcaagaat aggttttagc ccttccagaa tccgacaggt tatctctgtc
    30481 aggccctgtc ctggctgcat tatgttgaga tcgtagtcag gtacaatgga aaagagtttc
    30541 agcacctgat cgagcatctc ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa
    30601 ggatcttttg ccaacgcatg caccaacggc gccatcttga tggcttccgg gcgcgtacca
    30661 aatacagtca gtactttcac atcgattctc ttcgaataag cggcgagcgc ctttgcgctc
    30721 accgcagcag tgttgctatt tcgagcaacg gcgggttaat gcgacaccag ccccgatcag
    30781 ccccccgaca atgccccaca taatcatcag gaaggcacga cgtgggctat cgcgttttac
    30841 cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa cgcggatcca gggttggacc
    30901 aacattcagg gtgtttaaca tggcccgatt ctgatcatag tcgagatcaa aggccggacc
    30961 gacggcctgt aaattttcca gtcgagcctg aagcattgga cgcccaagca ggaacatttc
    31021 tgaatcaggt aattcctcgg caggcacatc tgtcgcactg cgcgaaatat tatgctgctc
    31081 agcaattttc agcgcctgct caatgctgtt catccggcgg tcgtagatgg ctttcgccac
    31141 ctcttcctga cgcttcacct gagctttcat ctggatggta cgcgccgccc atgcgccttt
    31201 cagctcatca ttcagatggc tggctgcacg ctggctggca aaagcaacat actgacgtaa
    31261 caggttatta gcgtcaggcg cggtttcggc aataagcttc acgctgtcat tgaccgcgcg
    31321 ggtaaagtct ccggggataa actggatgtt gttaatcatt tcatccagca acgccgcatc
    31381 ggctttgctg ttgcccacca tccgctgttt gtaatagtcg gtttgcagcc agaactctct
    31441 gcgggtatcc cacgaggcca gttgcataac aaactcttta taggcttcgt ccatgaccga
    31501 tggttggtcg gcagaagcca tgtttgaacg gacatccagg ttacgcaaaa attgctgctg
    31561 cgagtaatat ccccccagca tattcaccgt tggacgatcg gtaatcgccg tcgagctcca
    31621 ctcctgacga gcaaaaaacg tatacgccag cgcgattaac gcaaacgcca gccccatgcc
    31681 aataatccat agcttcccag cccacaaggt acgaaacaac ccacgaatat ccagttcatt
    31741 ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc atcacatcct catttatttg
    31801 gttaaattgg ggctgccacc acgatttcta cgcagtctgc gttttacgcg cttaataaag
    31861 cgagcaactt tccaggcacg cttaatgcaa tatccataga ggaagaatgc tagcaaaaag
    31921 agcaccagca tgacccactc cgggacaaaa tgagaatatt ctgccagcac gccaatggaa
    31981 gcgagcagtg ctgcggcaag ggtaatcagc acaaacgcct gacgggaagt aaacccggca
    32041 cgcatgatca aatggtgaat atgctgacgg tcaggagaga atgggctcat gcctttacgc
    32101 aggcgacggt acataatcgc caccatatcc attagcggaa tggcgattat ccacaaagcg
    32161 gtaaccgggc tgatgggatg ggttttgccc tgggtcgttt cgagcaggat ccagatcacg
    32221 gtaaaaccaa tcagcgtact gcccgcatca cccataaaga ctttgtagcg gcgacccagg
    32281 ataccaaggt taagcatgat gtatggcagg atggcggcga tcatcgcaaa gcaccagatt
    32341 gcgaggctgg tttgcccgtc gaaccacaaa atcataccga ttgctgcaaa cgagacgcag
    32401 gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc
    32461 cagacggcaa atagcgtcag gaagtaacca aacggtccga gcaccatctc ccaggagcca
    32521 aagatataac ccaggctact gagataaagc ttgccgaaca ccatcataac aatgccaaca
    32581 gcggcctgta tggtggcacg gatttttacg ctgatatcaa aacggtcatc cagcgcgcca
    32641 atgaaaacaa gcacaccggc acaagcgaga tagagagatg catgcggaat atagtaatcg
    32701 acaattccga acgtgaagca aatccctgcg taaaccgaaa tccccccaac gagaggtatc
    32761 aatccctggt gacgtttgcg gaagtttggt ttatccacta aaccgacttt ttttgccacc
    32821 ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa tactgatgag atcagtactc
    32881 actgtcagta aattcacaat gcatgctctc agagaaaatt attagcagaa gtataaccac
    32941 gaagaccttt attcagaagg gaaagtccgt tccgaaccca ccagtcctgt gcaaaaatta
    33001 accatttgat atgcaaattg cataacatgc cagcaaattc caggaatttt cctaacatcg
    33061 ccagcgcggc attaagatta cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt
    33121 ttggcataag acaaatttat gagcgtttca tcatttcgaa gaaatcgtca ttggtcttgg
    33181 tcatggccag tttattaatg aggaattcca ttgcatcgat ttcgcccatc gggtgaatga
    33241 ttttgcgcag gatccacatt ttctgcagtt cttcctgagt agtgagcagt tcttctttac
    33301 gggtaccgga acggttgtag tcgatagccg ggaagacgcg tttttcagcg atcttacgag
    33361 agaggtgcag ttccatgttg cctgtacctt taaactcttc gtaaataact tcgtccattt
    33421 tagaaccggt atcgataagc gccgtcgcga taatggtcag gctgccgccc tcttccacgt
    33481 tacgcgccgc accgaagaag cgtttcggac gatgaagggc attggcgtcc acaccaccgg
    33541 tcaacacttt acctgacgcc ggaacaacag tgttgtaagc gcgcgccaga cgagtgatgg
    33601 agtcgagcag aatgataacg tctttcttgt gctcaaccag gcgtttggcc ttctcgatca
    33661 ccatttccgc aacctgaacg tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt
    33721 caccttttac cagacgctgc atctcggtta cttcttccgg acgttcgtcg atcagcagaa
    33781 ccatcagcac acaatccggg tggttgtaag caatgctctg agcaatgttc tgcagcagca
    33841 tagttttacc ggctttcggc ggtgccacaa tcagaccacg ctgaccacga ccgataggtg
    33901 atgccagatc cagtacgcga gcagttaaat cttcagtaga accgttacca cgttccatac
    33961 gcagacgaga gtttgcgtgc agcggggtta agttctcaaa gaggattttg ttgcgggcgt
    34021 tttcaggttt gtcgaagtta acttcgttaa ctttcagcag cgcaaaatag cgttcacctt
    34081 ctttcggcgg gcgaatctta ccagagatgg tatcaccagt gcggaggttg aaacggcgga
    34141 tttggctagg ggaaacgtag atgtcatcag gaccggcgag gtaggagctg tctgcggaac
    34201 ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc atcaccaaag atatcttcgc
    34261 cactctttgc gtgctgcttc aggatggcaa aaataatgtc ctgcttacgc atacgagcca
    34321 ggttttccag ccccatattt tcgccgagag tgatcagctc agaaaccggc gtattcttta
    34381 attcggtaag attcatagtg gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg
    34441 ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg tcatcgactc atgtcttttc
    34501 gctgcctggt cacaggagag tacgcagaac tgaaacgaca agacggattg agtgacgagc
    34561 cagaaatctg ttcacttcgc atttaagata aaacgggaag cgttggaaat tacaagattc
    34621 aaacttaata aggtatgttt aatacgaagt caacactaag ttagcatgac tcacgccggg
    34681 cgtccagttt ttagcgacgg ggcacccgaa catgaaattc ccttacgcca ggttagcgtc
    34741 gaggaactct ttcaactgac ctttagacag tgcacccact ttggttgccg ccacttcacc
    34801 gtttttgaac agcagcagag tcgggatacc acggatgcca tatttcggcg cagtgccagg
    34861 gttttgatcg atgttcagtt ttgcaacggt cagtttgccc tgatattcgt cagcgatttc
    34921 atccagaatc ggggcgatca ttttgcacgg accgcaccac tctgcccaga aatcgacgag
    34981 gatcgccccg tccgctttga gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat
    35041 tttatcgctc atatataact ccacaggaat aagcctggcg tgttggtttc gttgttggtg
    35101 taacattaac caactaaagg ttgactttat ttcaccggat acgctttcgt aaagcaatag
    35161 taagctgata ttctaccaca ctatgagcaa aacacattta acagaacaga agttttccga
    35221 cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac
    35281 gcccattcag gcactggccc ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc
    35341 gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca acgtttcatt atcttctctc
    35401 tcatcctgcg attgccgatc gcaaggtgaa tcaaccacgt gccttaatta tggcaccgac
    35461 gcgtgaactt gccgtgcaga tccatgccga cgcagaaccg ctggcggaag ctaccggcct
    35521 gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa cagctgaaag tgctggaaag
    35581 cggcgttgac attctgattg gcaccacggg tcgtttaatt gactacgcca agcagaacca
    35641 cattaacctc ggtgccattc aggtagtggt actggacgaa gccgatcgca tgtacgatct
    35701 gggctttatt aaagatatcc gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct
    35761 caacatgctg ttctccgcca cgctttcgta ccgggtacgt gaactggcgt tcgagcagat
    35821 gaacaatgcc gaatatattg aagtggaacc ggaacagaaa acgggccacc gtattaaaga
    35881 agagcttttc tacccttcta acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga
    35941 agagtggcca gacagagcga ttattttcgc caataccaaa caccgttgtg aagagatctg
    36001 gggccacctg gcggcagatg gtcatcgtgt cggtttgttg acaggcgatg tcgcgcagaa
    36061 aaaacgtctg cgtattcttg atgaatttac ccgtggcgat ctggatattc tggttgccac
    36121 cgacgttgcc gcgcgtggtt tgcatattcc ggcagtgacg cacgtcttta actacgattt
    36181 acccgatgac tgtgaagatt acgttcaccg tattggtcgt acaggtcgcg caggcgcaag
    36241 cggtcactct atcagcctgg cgtgtgaaga gtatgcattg aatttgcctg ctattgaaac
    36301 ctatattggt cactcaattc cggtaagcaa atacaatccg gacgcattga tgaccgatct
    36361 gccaaaaccg ctgcgcctca cgcgcccgcg tacaggcaat ggtccgcgtc gtactggcgc
    36421 tccgcgtaat cgtcgtcgtt caggttaaat aaaatgtcat actcaaaatc attcaaactg
    36481 tatcaagatg gcaagagaat gaatcccgat gagcttaatg tagtaagtga ttcaggtgaa
    36541 agaatgcagc caacacagag acagattgaa ggatgaagag tatgggttcc acctcgtcgc
    36601 tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg cgcgaggtgg
    36661 ctggaagcat ccagacgctg acgcgaatta aacgcaaagt gcgtctggct gctggcctga
    36721 atagcgaaaa tgccctctct aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt
    36781 ttgctgaacg tctgcaagat atccctccct cgcaaattcg cgttgtcgct acggcgacat
    36841 tacgacttgc cgtcaatgcg ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc
    36901 cggtacaggt gatcagcggt gaagaggaag cgcgtctgat ttatcagggc gttgctcaca
    36961 ccactggtgg tgccgatcag cgcctggtgg tggatatagg cggtgccagc actgaactgg
    37021 taaccggcac gggtgcacaa accacctcgt tgttcagcct gtcgatgggc tgcgtcacct
    37081 ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat gctgcagaaa
    37141 aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac ggctggaaag
    37201 tgtgcgttgg cgcttccggc accgtgcagg cgttacagga aatcatgatg gcacagggga
    37261 tggatgaacg cattaccctg gaaaagttgc agcaattgaa acagcgagcc attcattgcg
    37321 gtcggctgga ggaactggag attgacgggc tgacgctgga acgtgcgtta gtgttcccga
    37381 gtggtctggc gatcctgatc gccattttta ccgaactgaa tattcagtgt atgaccctgg
    37441 cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt gcatctggcc gtcgagcagg
    37501 atattcgcag ccgtacgctg cgtaatattc agcgccgctt tatgatcgat attgatcagg
    37561 cgcagcgcgt agccaaagtt gcggctaact tcttcgatca ggtggaaaag gaatggcatc
    37621 ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa atcggcctga
    37681 gcgttgactt caaacaagcg ccgcaacacg ctgcttatct ggtgcgtaat ctggatcttc
    37741 ccggttttac ccccgcacag aaaaaattgc tggcgacgct actgctcaac cagactaatc
    37801 cggtcgatct ctcatcgctg catcagcaaa atgccgtacc gccgcgcgtc gcagaacaac
    37861 tctgccgttt actccgcctg gccatcattt ttgccagccg tcgccgtgac gatctcgtgc
    37921 cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg caaggttggc
    37981 taacccaaca tccgctgggt aaagagatta ttgctcagga aagccagtgg cagagctatg
    38041 tccactggtc gctagaagtg cattaatgag taagtgccgg atgcgatgct gacgcatctt
    38101 ctccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat tcgccagatg
    38161 gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct gttcccaaat
    38221 cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc tccgggcgca ccagctcgcc
    38281 gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc
    38341 gacataggcc agccgccgct cctcatcgat attatcttca tcgatgctgc tctggtgcgg
    38401 caaaaaccct tcttccatac cgaccatgta gacataagga aactccagcc cttttgacgc
    38461 gtgcagagtc atcagttgca cctgatccag ctcttcttca ctctcaccac gctccatcat
    38521 gtcgcgcaaa gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat ccagttcgct
    38581 gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca tgcgcatttc
    38641 ggcagctttc gggctgggcg atgtttcgta cagccaggat tcataatcca tgccgtggat
    38701 cagatcgcgc accgcggcaa tcggctcccg ctccgccaga cgctgaattt ctgccaacca
    38761 gtgagtgaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac tcaggcccat
    38821 atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccactcac ccagcttttt
    38881 cagcgtagcc gggccaatct ctcgcttcgg cgtgttaacg atacgcagaa atgcgctgtc
    38941 atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt caggacgaga
    39001 gaaaaacgac gtaccaccag aaattttgta cgggatgcgg ttttgcatca ggaatttttc
    39061 aaacacccgc gactgatggt taccgcgata aagaatggcg taatctttgt actgcgtttt
    39121 attgacgaag tgatgggcga tcagctcgcc agtaacgcgc tcggcttcat gttcctcgtt
    39181 attcgcgctt aaaactttta gctctgtgcc gtaacccagt tcggagaaca gacgcttttc
    39241 aaagacgtgc ggattattgg cgatcaggat gttcgccgct ttcaaaatac gtccggaaga
    39301 gcggtagttc tgctcaagct taatcacctt cagcgccgga aaatcctgac tcagcagcac
    39361 caggttttgt ggacgtgcac cgcgccagga gtagatcgac tggtcgtcat cgcccaccac
    39421 ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt tcatactggc tggtgttggt
    39481 gtcctgatac tcatccacca gcaggtagcg aattttgttc tgccagcgct cgcggacttc
    39541 ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga tcatcgaagt cgagaacgtt
    39601 acaggctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac ggtcccgctc
    39661 gccaatcgca cttgctgccg cctgggacgg tgttttgaga tcattcttcc agttagagat
    39721 ggtcgaaatc agttgttgca ggagaacttt gtcatcttca atcagcccct cggtcaactc
    39781 tttaagcaaa gcaagctgat cggtatcgtc aaacaacgag aagttcgctt tcatcccaag
    39841 cgccgcatac tcgcgtttga tgatatccaa ccccagcgtg tgaaaagtgg agatcatcag
    39901 cccgcgcgcc tctttgcgac ccagcgtctg cccgacacgc tctttcatct cgcgcgccgc
    39961 cttattggta aaggtcaccg ccgcgatatg tcgcgcctga taaccgcaac cgcggatcag
    40021 atgggcgatt ttattggtga taacacgagt tttaccggaa cccgcgcccg ccagcaccag
    40081 gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac gcataggtgt
    40141 attgctcaat cttcgaacgg ggggaggatt gtagcagaat cgcatttgaa tgtgaccgcg
    40201 tgtaagccgc taaccggttg gctgcggatg tttaaatcag agagggaaga ccgaaaccgg
    40261 agttaatcgt gaatccatta gacttagaaa atatcgggtc gtaacgttgt gttattactt
    40321 tttcagagtt tactaaggag aggtttactc gtggcgataa atttcagccc taaggttggt
    40381 gaaatactgg aatgcaactt tggtaactat cctgttagcc agaatggaca gttcagcacc
    40441 acgtattaca atggccgcat tcctcctgaa atgataaaaa acaggttagt tgtcgtgctc
    40501 aacggaaaaa ttaatggcaa cgcatgtatc gtcgttccat tatcaaccac tcgcgatcat
    40561 gacaagttaa atcgtggaat gcatgttgaa atagccagta atgttatcaa tgacctgcaa
    40621 ttctttgatc agcagattcg ttgggccaaa gccgacctgg tacaacaagt cagtcgaaac
    40681 cgattaaaca gagcaaggac ttacagaggg tatcttaatc aatgtttgcc acatgagttg
    40741 gttgcagaca tacagagggc ggtcatcaag tcaatcaatg ccatatcgct cataaattga
    40801 caataaaatc accatccccg ataatgactc tgtagcccat agaggcccta agttttctcc
    40861 ccttgaggga cctgcgaagc ccggattttc cgggcttttg ccatatatgt accagcccct
    40921 ccctgactga caattacgtt agcatctgcg gatcatgcta aaatcgccgc ctgacaatta
    40981 ttcactcaag gcacgatcat ggcaaaaaca tcagcagcac tgcatatcct tgtaaaagaa
    41041 gagaaactgg ctctggatct tctcgagcag attaagaacg gtgccgattt cggcaagctg
    41101 gcgaagaaac actccatttg cccatcaggc aaacgcggcg gtgatttagg tgaattccgc
    41161 cagggtcaga tggttccggc gttcgataaa gtggttttct cttgtccggt actggagccg
    41221 accggcccgc tgcacaccca gttcggatat cacatcatta aggtgctgta ccgcaactaa
    41281 tagcaaggcc ttctccagga gaaggccttg agtgttttct ccctctccct gtgggagagg
    41341 gtcggggtga gggcatcagc gcgcacttaa cccgcaacag caatacgttt catatctgtc
    41401 atatagccgc gcagtttctt acctacctgc tcaatcgcat ggctgcgaat cgcttcgttc
    41461 acatcacgca gttgcccgtt atctaccgcg ccttccggaa tagctttacc caggtcgccc
    41521 ggttgcagct ctgccataaa cggtttcagc aacggcacac aagcgtaaga gaacagatag
    41581 ttaccgtact cagcggtatc agagataacc acgttcattt cgtacagacg cttacgggcg
    41641 atggtgttgg caatcagcgg cagctcgtgc agtgattcat aatatgcaga ctcttcaatg
    41701 atgccggaat cgaccatggt ttcgaacgcc agttcaacgc ccgctttcac catcgcgatc
    41761 atcagtacgc ctttatcgaa gtactcctgc tcgccgattt tgccttcata ctgcggcgcg
    41821 gtttcaaacg cggttttgcc ggtctcttca cgccaggtca gcagtttctt atcatcgttg
    41881 gcccagtccg ccatcatacc ggaagagaat tcgccggaga tgatgtcgtc catatgtttc
    41941 tggaacaggg gtgccatgat ctctttcagc tgttcagaaa gcgcataagc acgcagtttc
    42001 gccgggttag agagacggtc catcatcagg gtgatgccgc cctgtttcag tgcttcggtg
    42061 atggtttccc aaccgaactg aatcagtttt tctgcgtatg ctggatcggt accttcttcc
    42121 accagcttgt cgaagcacag cagagagcca gcctgcaaca taccgcacag gatggtttgc
    42181 tcgcccatca ggtcagattt cacttccgca acgaaggacg attccagcac acccgcacgg
    42241 tgaccaccgg ttgcagccgc ccaggctttg gcaatcgcca tgccttcgcc tttcggatcg
    42301 ttttccgggt gaacggcaat cagcgtcggt acgccgaacc cacgtttgta ctcttcacgc
    42361 acttcggtgc ctgggcattt cggcgcaacc atcactacgg tgatatcttt acggatctgc
    42421 tcgcccactt cgacgatgtt gaaaccgtgc gagtagccca gcgccgcgcc gtctttcatc
    42481 agtggctgta cggtgcgcac tacatcagag tgctgcttgt ccggcgtcag gttaatcacc
    42541 agatccgcct gtgggatcag ttcttcgtaa gtacccactt taaaaccatt ttcggtcgct
    42601 ttacgccagg acgcgcgctt ctcggcaatc gcttctttac gcagagcgta ggagatatcg
    42661 agaccagaat cacgcatgtt caggccctgg ttcagaccct gtgcgccaca gccgacgatg
    42721 actacttttt taccctgaag gtagctcgcg ccatcggcga attcatcgcg gcccataaag
    42781 cgacatttgc ccagctgtgc cagctgctgg cgcagattca gtgtattgaa gtagttagcc
    42841 atggtgattc ctcgtgatgt tgtgcttctt attgttcggt tcgcgtttgc gagattgaat
    42901 tcactatatg acaggaaatt tattgcggaa attgatatat tcacaacgtc acattgcaat
    42961 ttttgcaacg tcaacatcga gggctgtccc tgtggattta cgcgatctga aaaccttcct
    43021 gcatctggcg gaaagccgcc attttggccg cagcgcgcgg gcgatgcacg ttagcccatc
    43081 cacgctctca cggcagattc agcgcctgga agaagatctc ggtcagccgc tgtttgtgcg
    43141 cgataaccgc acggtaacgc tgacggaagc gggcgaagag ctgcgcgttt tcgcccagca
    43201 aacgctgttg cagtatcagc aattgcgcca caccatcgat cagcaagggc cgtcgctctc
    43261 tggcgaatta catatcttct gctcggtgac cgctgcctac agccatctgc cgccgattct
    43321 ggatcgcttc cgcgcggaac acccgtcggt ggagatcaaa ctcaccactg gtgatgcggc
    43381 agatgcaatg gaaaaagtgg tcacgggaga agcggatctg gcgattgccg ggaaaccgga
    43441 gacgctaccc ggcgctgtgg cgttttcgat gctggagaat ctggcagtag tgctgattgc
    43501 gcccgcgctg ccctgcccgg tgcgtaacca ggtttcggca gagaagccgg actggtctac
    43561 agtgccgttt atcatggccg atcagggacc ggtgcgtcgc cgcattgaac tgtggtttcg
    43621 acgcaataaa atcagcaacc cgatgattta cgccacggtt ggcgggcatg aagcgatggt
    43681 atcgatggtg gcgctcggct gtggtgtggc gctgttgccg gaagtggtgc tggaaaacag
    43741 cccggaaccg gtgcgtaacc gcgtgatgat tttagagcgc agcgatgaga aaacgccgtt
    43801 tgagctgggc gtttgtgcgc agaaaaagcg gctacatgag ccgctgattg aagcattctg
    43861 gaagattttg ccgaaccaca aatgacgttg tcgcgcgggt aagcctgata agcgaagcgc
    43921 tatcaggcat ttttccctaa cccgccaaaa agaacctgaa cgccgggtta ttggtttcgt
    43981 cgtggcaatc gtagcccagc tcatttagcc gggtttcgaa atccggttca tggtcgccga
    44041 gttcgaacgc tgccagcacg cgcccatagt cggtaccgtg gctgcgatag tggaacaaag
    44101 aaatattcca gtgcgtaccc agcgtgttta ggaagcgcaa cagcgcgccc ggtgattccg
    44161 ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg ccgaccatat
    44221 agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg tagccgccgt
    44281 cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg cgcacgccga
    44341 caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg accgatcgcc
    44401 cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca
    44461 acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc aggccgtgga
    44521 agttcacgtt ggcaccggaa agaatatgcg ccagtcgttc gccgcgaatg ttgtgcaggg
    44581 cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc accgcgcgca
    44641 catcttcgaa taaatccttc atcgccgcac agatcgcatc actatcgacg gtgatgatgt
    44701 cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt accgctacgc
    44761 cttcagcaaa tagccctacg cgcggcagat caaccggatg acccgcatcc agcgctgctt
    44821 tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgtggc atcagttgtt
    44881 tgatcagcac cgcaacgccc gccgccagac cgccgccgcc gactggcaca aatacgcggt
    44941 cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg gcaatcacca
    45001 tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt tcgatcgctt
    45061 tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg aatccacgta
    45121 ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc ttcacgccta
    45181 accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca gtgatcacgc
    45241 cgtgcgcttt ctgttcttcc gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa
    45301 agctatgaac tggctgacga tcttcgcgct tcaccagaat gacgttatca agacgagacg
    45361 agagcttttc cattttttgt agcggcgtga cctgcgccgc ttcgtagacc ggcgcgcgca
    45421 gcaccgctct taaatattcg gcaccttccg gagcaccgga caggggttgt gagtcagcca
    45481 tcattaatcc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc tggtcgccag
    45541 gctggcgtag gcacgcaggg caaaggaaac ctgacgttca cggtttttcg gcgtccaggc
    45601 tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat cgcttacctg
    45661 taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt caatcaggcc
    45721 aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac cagaggtgcc
    45781 gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca ttgatttcag
    45841 gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc cttcatagcg
    45901 gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc
    45961 ctggctttca tatactttcg ccgggccggt gaatttgagg atgctgtcat cgacgcccgc
    46021 ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca ggccgccgtc
    46081 tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt catcgagaga
    46141 atcccaacgg caatcctgcg agaatgcctg tgtagtacga atgcccgcag ggcctgcgcg
    46201 gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgtt ccagcgtttg
    46261 cggcaacgtc aggccaagta cgtttttcac atcacggttc agtaaccccg cgcgatccag
    46321 ttcgccgaga ataccgataa caccaccagc acggtgaaca tcttccatat ggtatttctg
    46381 ggtgctcggc gcaactttac acagctgtgg aaccttgcgg gaaagcttat cgatatcact
    46441 catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta cggtgttagt
    46501 cgatccaccc atcgcgatat ccagcgtcat ggcgttttca aacgccgcct tactggcgat
    46561 attacgcggc agtgcacttt cgtcgttttg ctcgtaataa cgtttggtca attcaacaat
    46621 gcgtttacca gcattaagga acagctgctt acggtccgcg tgggttgcca gcagcgagcc
    46681 gttgcctggc tgcgacaaac ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa
    46741 catcccggag caggaaccgc aggttgggca ggcggaacgt tcaacctgat cgctctggga
    46801 gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga gcttgatgat
    46861 ctgctcggac agtttggttt tcccggcttc catcgggccg ccggaaacaa agatcaccgg
    46921 aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc agttagagat
    46981 gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat cagcgatcag
    47041 ttcgcgagat ggcagtgaat aaagcatccc cccgtggccc atagcaatcc catcatccac
    47101 cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt cggcgaccag
    47161 tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg agttcacaac
    47221 cgcgataatc ggcttgccaa aatcggcatc ggtcattccg gtggcgcgcc acagcgcacg
    47281 agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag gcatacttta
    47341 tttactccca gtgtctgtct cgtaaatggg acggtgagtg ccgtcccatt ttttgtattt
    47401 attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccggta aagaggccga
    47461 agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca acctgaatac
    47521 cgtctacgct gcgcactggc gtgatttctg ccgctgtccc agacataaac acttcatccg
    47581 ccaggtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc agctctttcg
    47641 ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg
    47701 tgaacagcac accatctttc acttcaaaca ggttttcgcc tgcgccttca gagatataac
    47761 cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca
    47821 gcagggaaga gagatagtta ccaccggctt ttgccgcagt tgggatggtg tttggtgctg
    47881 cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct gcgcccagat
    47941 acgcccccca cgggaaagcg gcgataatca cgtcggttga gtatcccgct ggcgggttta
    48001 cgcccatgcc aacatcacca acgaagatca gcggacggat ataggcgctg gtgagattgt
    48061 ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgc gaaaccggga
    48121 agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg cggaatacaa
    48181 ccggtccttt gtgcgagtcg tagcaacgga tgccttcaaa aaccgaggtt ccatagtgca
    48241 gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc ccattgaacc
    48301 aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca ggcgcggatt
    48361 tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac cagtttattt
    48421 aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc gatatttata
    48481 ttttgtgcat cgctggcggc ggccatattc attgagcaga cgtggaaacc acgatgacgc
    48541 accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac attgacctga
    48601 tgttgcatca tgataatttc tccaacattt ctgaattact ggcacctggc ggcaccagcg
    48661 gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca tcactgttca
    48721 gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg ccagggatgc
    48781 cgaaggcgct ggctaacatg aggaaatcgg ggttatcggt aagggtggtt tcgctgtatc
    48841 gttcctggaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg ttatcgagta
    48901 agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc acattcatca
    48961 tgaaagagcc gtcaccggag atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc
    49021 caaccgccgc cggtaaacca aaacccatgg tacctaaacc gctggaggtg atgaaatttt
    49081 ccgggcgagt gtgggcgatg tgctgcgcag cccacatctg gtgctgcccc acatctgtgg
    49141 tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac ggcgcgtaga
    49201 tagcgtcacc gggatggtcg taacgccagg aatgttcatc acgcagctgc gcgcagtgtt
    49261 gctgccagtc attgatattt aacggctgct gtaatgctgg taacagagca tttaaatcac
    49321 cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg atatccatat
    49381 ggataacact ggcgtgtggc gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac
    49441 gtgcgcccac ggcgatcagc aggtcacact cctgcaccgc gaagtttgcc gctttggtgc
    49501 cgtgcatccc cagcatgccc agatagtacg gataatctgc ttctactgcg cccagccctt
    49561 tcagcgtaca ggtggcaggc atttttgtgg cagcgagaaa ttcacgcaaa gccggaactg
    49621 cctgcgccat acccacgcca ccgccaacgt acagcatcgg tttttgcgct tttgccagca
    49681 tctggcgcgc ttgctcaact tcggcatgtg ggaaagtcac ttcgttttca acggtggtga
    49741 accacggttc caggtcaccg ctggctaact ggatatcttt tgggatatcg accagaaccg
    49801 gaccaggacg acctgagcag gcaacgtcga atgcttcagc catgatgcgc ggcaactctt
    49861 ccagcgactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc aggacatcca
    49921 cttcctgaaa tgcgtccgtg ccgataaacg gtgcggacac ttgaccggtg atggcaacaa
    49981 cagggataga atctaacagt gcgtccgcaa gcccggttat caggttggtt gcgcccggac
    50041 cagacgtggc gatacatacg ccagttttgc cggtagcacg ggcataaccg atagccgcca
    50101 ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca tacaatgcat
    50161 cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc
    50221 gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtcc tgaatcttga
    50281 gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt taaggtcaaa
    50341 aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga tctctaagcc
    50401 tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac gctaatcacg
    50461 accaggctaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc ttgttcgaag
    50521 gaatgcctaa agagttacca tagattttgc gaatagcaca agatattttt tggccgattt
    50581 ttatgatgcg tttaataatt tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt
    50641 gaaaaaaatt caatttcagt atgaatgttg ttcccttcct agtagttcat cctttgtccg
    50701 ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt gcacaaataa
    50761 acaggaatca gccactaaaa ttcacgaatt agcgggctac agcagcgata tcttatgcac
    50821 ataatagtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg cgcagccctg
    50881 ggagtaaatg cgcccccaat cactgttgag gtacatatca gtaaaggtct acccggctta
    50941 acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt gcgcagcgcc
    51001 attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct ggctccagcc
    51061 gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt gctggcggcc
    51121 tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga actggcgctt
    51181 acaggcgctc tgcgtggcgt tcccggcgca atctccagtg caactgaagc tattaagtcg
    51241 ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat taacggtgaa
    51301 ggatgcctga tagccgatca tctgcaggct gtctgtgcgt ttctggaagg taagcacgct
    51361 ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga tctcagtgat
    51421 gttatcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg cgggcacaac
    51481 cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg tattaatggc
    51541 ctgttgccag atttaagcaa tgaagaggca ctggagagtg ctgcgatatt aagtctggta
    51601 aatgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc tcatcacagt
    51661 gcatcgttaa ctgcgatggt aggcggtggc gcaattccag ggcccggtga aatttcgctg
    51721 gcgcataacg gcgtgctttt tcttgatgag ctacctgaat ttgaacggcg tacactggat
    51781 gccttgcgag agccgattga atccgggcag atccatcttt cacgcacacg agcaaaaata
    51841 acctatccag cccgtttcca gcttgttgcg gcgatgaatc ccagccctac cggacattat
    51901 cagggaaacc ataaccgctg cacgccagaa cagacattac gttatctcaa ccggctctcg
    51961 gggccctttc tcgaccgctt cgatctctca ctggagatcc cattaccacc ccccggcatt
    52021 ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg cgtaatggcc
    52081 gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga tagtccggaa
    52141 atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaagg aacactgatc
    52201 catctggggt tatcgattcg tgcctggcag cggttattga aagttgcacg aaccattgct
    52261 gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt tagctatcga
    52321 gcgattgacc gtttgctcat ccatctgcag aaactactga cataaaaaaa gggcatttcg
    52381 ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct gcggtttacc
    52441 gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact tggaccacac
    52501 gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga agagtttctc
    52561 ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat aaccatgacg
    52621 ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc cacgtggata
    52681 atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc tgcacgctcc
    52741 taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac agagtgtaaa
    52801 acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg atacggaatt
    52861 gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg ctgaatcgct
    52921 ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc aactgggtgt
    52981 gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg aaaaactact
    53041 gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgtaaggagg tggcgcatac
    53101 ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat gtatgcttaa
    53161 tcagtggctg ggacgcttgt atcaaaatca ggatgcccat acaggcttac agttcgaagc
    53221 gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc ttgatcttct
    53281 tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg gatatttcac
    53341 tttagcgctt tataccagtg ccccttcaaa actaaaggga gatcttaatt atctgcgact
    53401 tgagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg acgaagtgcc
    53461 cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc ttaatggttg
    53521 cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg ttgtcgatag
    53581 cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata aaaatgcgtt
    53641 gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatatg aatggctggc
    53701 aaggatgccg gtagaaggat ttacttcgga gagggttatt tcagataaaa aaaatcctta
    53761 gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca acacccggtt
    53821 ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg gaacggacgg
    53881 gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
    53941 ccaccgaatt cttttacaac caccggtttt atgaccggct tactgcttaa tttgatgcct
    54001 ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
    54061 cttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaattct
    54121 gttttatcag accgcttctg cgttctgatt taatctgtat caggctgaaa atcttctctc
    54181 aatccgccaa aaca
//
LOCUS       DAZFZC010000020        53728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFZC010000020 DAZFZC010000000
VERSION     DAZFZC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.020"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1332)
                     /locus_tag="ACHKXP_001436"
     CDS             complement(<1..1332)
                     /locus_tag="ACHKXP_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646475.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNIARIAGGED
                     SRSNILSPGAFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMQF
                     VMNNPEGERADSPKVIIEVSLSTITTMGTRQGHTAIIFPQPDGSTNRYEGKSFERKDE
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEINNIKINLSNILI
                     DSLDDAKVNLEKVIDSMLKTFLKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     LFDDVKLLFNEVSVSILVNDIFLKDFFMKQPDFAKWYFYQLLKKYEGEQLYL"
     gene            complement(1455..1907)
                     /gene="tabA"
                     /locus_tag="ACHKXP_001437"
     CDS             complement(1455..1907)
                     /gene="tabA"
                     /locus_tag="ACHKXP_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO7646476.1"
                     /translation="MIFGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(2052..2645)
                     /gene="bdcR"
                     /locus_tag="ACHKXP_001438"
     CDS             complement(2052..2645)
                     /gene="bdcR"
                     /locus_tag="ACHKXP_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7646477.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            2716..3429
                     /gene="bdcA"
                     /locus_tag="ACHKXP_001439"
     CDS             2716..3429
                     /gene="bdcA"
                     /locus_tag="ACHKXP_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7646478.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3560..3955
                     /gene="yjgH"
                     /locus_tag="ACHKXP_001440"
     CDS             3560..3955
                     /gene="yjgH"
                     /locus_tag="ACHKXP_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7646479.1"
                     /translation="MVERTAVFPAGQHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4236..4370
                     /gene="pyrL"
                     /locus_tag="ACHKXP_001441"
     CDS             4236..4370
                     /gene="pyrL"
                     /locus_tag="ACHKXP_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO7646480.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4374..5309
                     /gene="pyrB"
                     /locus_tag="ACHKXP_001442"
     CDS             4374..5309
                     /gene="pyrB"
                     /locus_tag="ACHKXP_001442"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO7646481.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5322..5783
                     /gene="pyrI"
                     /locus_tag="ACHKXP_001443"
     CDS             5322..5783
                     /gene="pyrI"
                     /locus_tag="ACHKXP_001443"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO7646482.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5856..6242
                     /gene="ridA"
                     /locus_tag="ACHKXP_001444"
     CDS             5856..6242
                     /gene="ridA"
                     /locus_tag="ACHKXP_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO7646483.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6448..9144)
                     /gene="mgtA"
                     /locus_tag="ACHKXP_001445"
     CDS             complement(6448..9144)
                     /gene="mgtA"
                     /locus_tag="ACHKXP_001445"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO7646484.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9285..9338)
                     /gene="mgtL"
                     /locus_tag="ACHKXP_001446"
     CDS             complement(9285..9338)
                     /gene="mgtL"
                     /locus_tag="ACHKXP_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO7646485.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9523..10470
                     /gene="treR"
                     /locus_tag="ACHKXP_001447"
     CDS             9523..10470
                     /gene="treR"
                     /locus_tag="ACHKXP_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO7646486.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10589..12010
                     /gene="treB"
                     /locus_tag="ACHKXP_001448"
     CDS             10589..12010
                     /gene="treB"
                     /locus_tag="ACHKXP_001448"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO7646487.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVDGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12060..13715
                     /gene="treC"
                     /locus_tag="ACHKXP_001449"
     CDS             12060..13715
                     /gene="treC"
                     /locus_tag="ACHKXP_001449"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO7646488.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            14109..16247
                     /gene="nrdD"
                     /locus_tag="ACHKXP_001450"
     CDS             14109..16247
                     /gene="nrdD"
                     /locus_tag="ACHKXP_001450"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO7646489.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDKWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16406..16870
                     /gene="nrdG"
                     /locus_tag="ACHKXP_001451"
     CDS             16406..16870
                     /gene="nrdG"
                     /locus_tag="ACHKXP_001451"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO7646490.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16915..17301)
                     /gene="cybC"
                     /locus_tag="ACHKXP_001452"
     CDS             complement(16915..17301)
                     /gene="cybC"
                     /locus_tag="ACHKXP_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO7646491.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17356..18708)
                     /gene="pmbA"
                     /locus_tag="ACHKXP_001453"
     CDS             complement(17356..18708)
                     /gene="pmbA"
                     /locus_tag="ACHKXP_001453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO7646492.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFEAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLSEMKIAGQ
                     "
     gene            18802..19353
                     /gene="yjgA"
                     /locus_tag="ACHKXP_001454"
     CDS             18802..19353
                     /gene="yjgA"
                     /locus_tag="ACHKXP_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO7646493.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19509..20882)
                     /gene="mpl"
                     /locus_tag="ACHKXP_001455"
     CDS             complement(19509..20882)
                     /gene="mpl"
                     /locus_tag="ACHKXP_001455"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO7646494.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21058..22056
                     /gene="fbp"
                     /locus_tag="ACHKXP_001456"
     CDS             21058..22056
                     /gene="fbp"
                     /locus_tag="ACHKXP_001456"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO7646495.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22089..23084)
                     /gene="yjfF"
                     /locus_tag="ACHKXP_001457"
     CDS             complement(22089..23084)
                     /gene="yjfF"
                     /locus_tag="ACHKXP_001457"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO7646496.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23071..24093)
                     /gene="ytfT"
                     /locus_tag="ACHKXP_001458"
     CDS             complement(23071..24093)
                     /gene="ytfT"
                     /locus_tag="ACHKXP_001458"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO7646497.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGAIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24107..25611)
                     /gene="ytfR"
                     /locus_tag="ACHKXP_001459"
                     /pseudo
     CDS             complement(24107..25611)
                     /gene="ytfR"
                     /locus_tag="ACHKXP_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
     gene            complement(25751..26707)
                     /gene="ytfQ"
                     /locus_tag="ACHKXP_001460"
     CDS             complement(25751..26707)
                     /gene="ytfQ"
                     /locus_tag="ACHKXP_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO7646498.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27017..27547
                     /gene="ppa"
                     /locus_tag="ACHKXP_001461"
     CDS             27017..27547
                     /gene="ppa"
                     /locus_tag="ACHKXP_001461"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO7646499.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27927..28268)
                     /gene="ytfP"
                     /locus_tag="ACHKXP_001462"
     CDS             complement(27927..28268)
                     /gene="ytfP"
                     /locus_tag="ACHKXP_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO7646500.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28271..32050)
                     /gene="tamB"
                     /locus_tag="ACHKXP_001463"
     CDS             complement(28271..32050)
                     /gene="tamB"
                     /locus_tag="ACHKXP_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO7646501.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDVGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32047..33780)
                     /gene="tamA"
                     /locus_tag="ACHKXP_001464"
     CDS             complement(32047..33780)
                     /gene="tamA"
                     /locus_tag="ACHKXP_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO7646502.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLKKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33986..34624
                     /gene="msrA"
                     /locus_tag="ACHKXP_001465"
     CDS             33986..34624
                     /gene="msrA"
                     /locus_tag="ACHKXP_001465"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO7646503.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34947..36290
                     /gene="paeA"
                     /locus_tag="ACHKXP_001466"
     CDS             34947..36290
                     /gene="paeA"
                     /locus_tag="ACHKXP_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO7646504.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36352..36558)
                     /gene="ytfK"
                     /locus_tag="ACHKXP_001467"
     CDS             complement(36352..36558)
                     /gene="ytfK"
                     /locus_tag="ACHKXP_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO7646505.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36883..37440
                     /gene="ytfJ"
                     /locus_tag="ACHKXP_001468"
     CDS             36883..37440
                     /gene="ytfJ"
                     /locus_tag="ACHKXP_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7646506.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37430..38170)
                     /gene="cysQ"
                     /locus_tag="ACHKXP_001469"
     CDS             complement(37430..38170)
                     /gene="cysQ"
                     /locus_tag="ACHKXP_001469"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO7646507.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPEIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38360..40323
                     /gene="cpdB"
                     /locus_tag="ACHKXP_001470"
                     /pseudo
     CDS             38360..40323
                     /gene="cpdB"
                     /locus_tag="ACHKXP_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(40451..40831)
                     /gene="ytfH"
                     /locus_tag="ACHKXP_001471"
     CDS             complement(40451..40831)
                     /gene="ytfH"
                     /locus_tag="ACHKXP_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO7646508.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40920..41783
                     /gene="qorB"
                     /locus_tag="ACHKXP_001472"
     CDS             40920..41783
                     /gene="qorB"
                     /locus_tag="ACHKXP_001472"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7646509.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQQIVAIVRNPAKAQALAA
                     QGITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAY
                     TSLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIG
                     AAGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQV
                     TYQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLA
                     ESVSHLFNVNK"
     gene            41892..42857
                     /gene="ytfF"
                     /locus_tag="ACHKXP_001473"
     CDS             41892..42857
                     /gene="ytfF"
                     /locus_tag="ACHKXP_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7646510.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            42965..43627
                     /gene="ytfE"
                     /locus_tag="ACHKXP_001474"
     CDS             42965..43627
                     /gene="ytfE"
                     /locus_tag="ACHKXP_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO7646511.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43672..45084)
                     /gene="cycA"
                     /locus_tag="ACHKXP_001475"
                     /gene_synonym="dagA"
     CDS             complement(43672..45084)
                     /gene="cycA"
                     /locus_tag="ACHKXP_001475"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO7646512.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPDKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(45386..46006)
                     /gene="fklB"
                     /locus_tag="ACHKXP_001476"
     CDS             complement(45386..46006)
                     /gene="fklB"
                     /locus_tag="ACHKXP_001476"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7646513.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLQGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            46225..46863
                     /gene="ytfB"
                     /locus_tag="ACHKXP_001477"
     CDS             46225..46863
                     /gene="ytfB"
                     /locus_tag="ACHKXP_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO7646514.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46847..47494)
                     /locus_tag="ACHKXP_001478"
     CDS             complement(46847..47494)
                     /locus_tag="ACHKXP_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7646515.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYITNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            47707..48498
                     /locus_tag="ACHKXP_001479"
     CDS             47707..48498
                     /locus_tag="ACHKXP_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO7646516.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNLWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            48508..49350
                     /locus_tag="ACHKXP_001480"
     CDS             48508..49350
                     /locus_tag="ACHKXP_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7646517.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPQGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPELASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            49360..50136
                     /locus_tag="ACHKXP_001481"
     CDS             49360..50136
                     /locus_tag="ACHKXP_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO7646518.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            50146..51687
                     /locus_tag="ACHKXP_001482"
     CDS             50146..51687
                     /locus_tag="ACHKXP_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7646519.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPSVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            51684..>53728
                     /locus_tag="ACHKXP_001483"
     CDS             51684..>53728
                     /locus_tag="ACHKXP_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO7646520.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEPVPGDLPFSLERLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAQA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIVIDPQGKCLP
                     QTRRGAKEEWRFRSERAEDKNHRLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPD"
BASE COUNT        13233 a        14142 c        13478 g        12875 t
ORIGIN      
        1 aagatacagc tgttcgccct catacttctt aagtaattga tagaagtacc acttcgcaaa
       61 atcaggttgc ttcatgaaaa aatccttcaa aaaaatatca tttactaaaa tgctgacaga
      121 cacttcatta aatagcaact taacatcatc aaataacctt agtattaagc gtgatttttc
      181 ttgattgctt aagttaaaat cattgacata tacaaccaac ttctcccaaa tcaattcctg
      241 taactcaggg ctgtttttat agggttcttt attgaggttg aaaaataatg tctcagctat
      301 tttagactga tcgcagttaa aatcaatatg atcgatcacg gagcgggcat gctttatctt
      361 tgtagtatca tcagaatatt gcatactttg attaaaacac cactccagaa tttttacatc
      421 attaatatat ggcaatttta aaaaagtctt caacatcgaa tcgatgacct tttcgagatt
      481 aacttttgca tcatctaagg aatcaataag aatattagac aggttaattt tgatgttatt
      541 aatttcatca ttaccttctg taccactagc aattctggta tcatgtaacg ctttcagatc
      601 atttatttct tcaagtatac caccttcgcc tagtaagtat ggaatcgtgt catcacatac
      661 atgaggcatt aatagcagat gttctttctt tattttttct aatgtactgg ctaatggctt
      721 ttctaataac tcgttataat caattgcgtc cttccagttc aactgatgat tatttaatag
      781 aagcgccata ttcttgttag cttcgcgttg gtaacacgcc agaaccttgt tagtaatcag
      841 atgtaatgaa ctctcatctt ttctttcaaa ggacttccct tcataacggt tagtcgaacc
      901 atcaggttgt ggaaatataa tggctgtatg tccttgacga gtccccatag tagtgatagt
      961 ggaaagtgaa acttctataa tcaccttggg tgaatccgct cgctcacctt caggattatt
     1021 cataacaaac tgcattattt cactgccatc attatttttg atataagatg aggactcaat
     1081 atgagccatc atatttctac ccgcttcgtt ggagcgtttg ctaaaatcca cataacccag
     1141 ggtaaatatt tttaccaaaa actccaaaaa tgctccagga gatagaatat tgcttctgct
     1201 atcctccccc ccggcaatac gggcaatatt attcattcca acacgccagg tatttaaaac
     1261 ttcacttttt tgtttaaaat tgtcggctaa tgtaatgtgt tgagaataat tcaaatctga
     1321 tattttgctc atgacgaact ccttgagtta ataagaacgt ctaagcatta caaattttac
     1381 tcacaaaaaa atatcaaaaa acgctctttt cacaaactcg ccgtaaacct ggagttattt
     1441 acggcgaaaa gtcatcacgc catcagcatc ttcactactg ctttgcgaac ccgggctggc
     1501 gcgcccactg cgcacagcgg tttatgcact tcccccggat aaaacacaac aaaatcacct
     1561 tcattaagga taactgtttt ctcatcaacg ccttccggca aaaatgcgat gtctttatca
     1621 gctaaccagt cggtatccgg cgtgcctgca ggttgcgtgc tgaaggtcat gccttcctga
     1681 ccttttaaca caatctgaat gtcgagatag cgggcatggt actccgcacg gcgagcttcg
     1741 tacggctcgg tcatatcttc cgagataaga taaaacagac gattgccttc gatatcgtgc
     1801 ttaccctttg gcgtttctgc cgtaacgtgt gctttgatat gctcaatcgc ctggcgtaac
     1861 tcctggggta gccacggctg aagattatga atatttccaa agatcatctt tttcactcct
     1921 taattaaaac aatgtttcat ttccaccgtt atacgaaaag atgatcattt ccgctagcct
     1981 taacgcgtga attattgtct tatcacaact tatcggcagg cgcgttcccg cctttcggat
     2041 caaggccagc atcactcctt gagaatagtt tttatcgctt cccccgccag tgcagctgtt
     2101 gcacagagtt gttcgaggga gtgcccctct cgtgccttcg cagaaaggcc tgacatcacg
     2161 gtactcataa aatcagtcac acattgtgcg ctttgtggat gccgcctggc gatatagtca
     2221 taaatggtcg tttctgcggc gtgataatat tgaacggcaa tatcacgcgc ttgtggatca
     2281 tgactatgaa taccttcaag aaccatacag ccagcgcagc cgccgttttg gctatatctg
     2341 cgcgccgctt cttttaatac ctcaaccagg cactcgccta ccggacgatc atcacgaaga
     2401 atatcggcaa gcggaatagc ttccgtaccg acatattcat tgagtacacg gctaaataac
     2461 ccagccttac tgccaaaagc cgcgtagagg ctcggggggt taataccaag ataatcagta
     2521 acttcagcaa cactgacagc atcgaaacct ttttggtgaa aaagcacttt tgccgcagag
     2581 actgcctgct caggagcgaa acgtctggga cgacctggaa cgcgagattg ttttttagtg
     2641 accattacaa aacttgttga cagaaagtta aaacagtttt gtaatgcatg ttacataata
     2701 aatcaaggag tccttatggg cgcttttaca ggtaagacag ttctcatcct cggtggtagc
     2761 cgtggaatcg gtgccgctat tgtacgtcgt ttcgtcaccg atggggccaa tgtacgattc
     2821 acctatgcgg ggtcgaaaga tgccgctgaa cacctggcac aagagactgg agcgacagca
     2881 gtattcacag atagtgctga cagagacgct gtcattgatg tcgttcgtaa gagtggcgca
     2941 ttggatattc tggtagtaaa tgcaggtatt ggcgtctttg gcgaggccct ggaattaaat
     3001 gccgacgata ttgatcgcct tttcaaaatc aatattcatg ctccttatca tgcctctgtt
     3061 gaagccgccc ggcagatgcc cgaaggcggg cgcatcttaa tcatcggctc cgtgaatggc
     3121 gatcgtatgc ctgttgcagg catggctgct tatgccgcca gtaaatctgc cctgcaaggc
     3181 atggcgcgcg ggctggcccg tgatttcgga ccgcgtggga tcaccatcaa cgtcgtccag
     3241 ccagggccaa ttgataccga cgctaatccc gccaacgggc caatgcgcga tatgttgcat
     3301 agttttatgg ctatcaaaag acatgggcaa ccggaagagg tcgctggtat ggtcgcatgg
     3361 ttagcagggc cagaagccag ctttgttacc ggcgcgatgc ataccattga tggcgcgttt
     3421 ggcgcataac cgactacgct caattaagcc cagccattat ccatgatgtc tgggttttgt
     3481 ttactcacgt cgtccgctaa aagcggctcc cggtaaatag aaatcttctg ttcatgtcgt
     3541 aaatcttgag gataaccaaa tggtagaaag aaccgctgtt ttccctgctg gccaacattc
     3601 actgtatgca gaacatcgtt attctgcggc tattcgttcc ggcgatttgc tgtttgtttc
     3661 cgggcaagta ggaagtcgag aggacggaac accagaaccc gattttcagc aacaagtcag
     3721 actggcattt gataatttgc atgcgaccct ggctgctgcg ggatgcactt ttgacgatat
     3781 cattgatgtt acgagcttcc ataccgatcc agaaaaccaa tttgaagaca tcatgacggt
     3841 gaaaaatgaa atatttagcg ccccacctta tccaaactgg acggcggtag gtgttacatg
     3901 gctggcaggc tttgattttg aaattaaagt gatagcgcgc atccctgagc agtaagcaat
     3961 agtgttagcc gttcgttttc acactccgcc ctataagtcg gatgaatgga ataaaatgca
     4021 tatctgattg cgtgaaagtg aaaaagaaaa aagcaggaaa tgtctgcaat tattgatacc
     4081 gaacgaccgt tcccctgcag aatcacaaca aataaaaatg catgtatttt gacttataat
     4141 tcaaataaac cgtttgcgct gacaaaatat tgcatcaaat gcttgcaccg cttctgacga
     4201 tgagtataat gccggacaat ttgccgggag gatgtatggt tcagtgtgtt cgacattttg
     4261 tcttaccgcg tctgaaaaaa gacgctggcc tgccgttttt cttcccgttg atcacccatt
     4321 cccagcccct caatcgaggg gctttttttt gcccaggcgt caggagataa aagatggcta
     4381 atccgctata tcagaaacat atcatttcca taaacgacct tagtcgcgat gaccttaatc
     4441 tggtgctggc gacagcggcg aaactgaaag caaacccgca accagagctg ttgaagcaca
     4501 aagtcattgc cagctgtttc ttcgaagcct ctacccgtac ccgcctctct ttcgaaactt
     4561 ccatgcaccg cctgggtgcc agcgtggtgg gcttctccga cagcgccaat acatcactgg
     4621 gtaaaaaggg cgaaacgctg gccgatacca tttcggttat cagcacttac gtcgatgcga
     4681 tagtgatgcg tcatccgcag gaaggtgcgg cgcgcctggc caccgagttt tccggcaatg
     4741 taccggtact gaatgccggt gatggctcca accaacatcc gacgcaaacc ttgctggact
     4801 tattcaccat tcaggaaacc caggggcgtc tggacaatct ccacgtcgca atggttggtg
     4861 acctgaaata tggccgcacc gttcactccc tgactcaggc gttagcgaag ttcgacggca
     4921 accgttttta cttcatcgcg ccggacgcgc tggcaatgcc gcaatacatt ctggatatgc
     4981 ttgatgaaaa agggatcgcc tggagtctgc acagctctat tgaagaagtg atggcggaag
     5041 tagacatcct gtacatgacc cgcgtgcaaa aagagcgtct ggacccgtcc gagtacgcca
     5101 acgtgaaagc gcagtttgtt cttcgcgcca gcgatctcca caacgccaaa gccaatatga
     5161 aagtgctgca tccgctgccg cgtgttgatg agattgcgac ggatgttgat aaaacgccac
     5221 acgcctggta cttccagcag gcaggcaatg ggattttcgc tcgccaggcg ttactggcac
     5281 tggttctgaa tcgcgatctg gtactgtaag gggaaataga gatgacacac gataataaat
     5341 tgcaggttga agctattaaa cgcggcacgg taattgacca tatccccgcc cagatcggtt
     5401 ttaagctgtt gagtctgttc aagctgaccg aaacggatca gcgcatcacc atcggtctga
     5461 acttgccttc tggcgagatg ggccgcaaag atctgatcaa aatcgaaaat acctttttga
     5521 gtgaagatca ggtagatcaa ctggcattgt atgcgccgca agccacggtt aaccgtatcg
     5581 acaactatga agtggtgggt aaatcgcgcc ccagtctgcc agagcgcatc gacaatgtgc
     5641 tggtctgccc gaacagcaac tgtatcagcc atgccgaacc ggtttcttcc agctttgccg
     5701 tgcgaaaacg cgccaatgac atcgcgctca aatgcaaata ctgtgaaaaa gagttttccc
     5761 ataatgtggt gctggccaat taattgcggt tggtaataaa agtctggctc cctataatga
     5821 gccagacttt ttaccgctgt aataaaggag aaatcatgag caaaactatc gcgacggaaa
     5881 atgcaccggc agctatcggt ccttacgtac agggcgttga tctgggcaat atgatcatca
     5941 cctccggtca gatcccggta aatccgaaaa cgggtgaagt accggcagac gtcgctgcac
     6001 aggcacgtca gtcgctggat aacgtaaaag cgatcgtcga agccgctggc ctgaaagtgg
     6061 gcgacatcgt taaaaccacc gtgtttgtga aagatctgaa cgacttcgct accgttaatg
     6121 ctacctacga agccttcttc accgaacaca acgccacctt cccggcacgt tcttgcgttg
     6181 aagtcgcccg tctgccgaaa gacgtgaaga ttgagatcga agcgatcgct gttcgtcgct
     6241 aatcttgatg gaaatccggg ctatcatgcc cggattaagt ctgatgacaa acgcaaaatc
     6301 gcctgatgcg ctacgcttat caggcctacg tgattcctgc aatttattga atttgttggc
     6361 cggataaggc atttacgccg catccggcat gaacaaagct tactttgtct gcaatctgaa
     6421 tcggggctat cgtgcccagt ttattcttta ttgccagccg taacgacggc tatagaaccc
     6481 tttcaccaac tgggttaatg tcatataccc tgccagaatc gcaaccagcc acgggaaata
     6541 gcttaacggc agcgcctgta attgcagata actggccagc ggtgaaaacg gcaatgcgat
     6601 cccgacaatc atcacgatca cggtcatgat cattaacggc cacgatgcac agctctgaat
     6661 aaacggcaca cggcgggtgc ggatcatatg cacaatcagc gtttgcgaca gtaagcccac
     6721 cacaaaccat cccgactgga acagcgtttg cgtttccggc gtgttggcat ggaataccca
     6781 ccacatcagg caaaacgtca gaatatcgaa gatcgagctg atcggtccga agaagatcat
     6841 aaagcgcccc agatccgccg gattccaacg ctgcggcttt tgaatttgct cgtcgtcgac
     6901 gttatcaaac gggatcgcca cctgtgacac atcatacagc aggttctgaa taagcaagtg
     6961 taacggcaac atcggcagga agggcaagaa agcactcgcc accagcacgc tgaacacatt
     7021 accgaagttg gagctcgccg tcattttgat gtatttcagc atgttggcga aagtgcgacg
     7081 tccttcaata accccctctt ccagcaccat caggcttttt tccagcagga tgatatcagc
     7141 cgcttcacgg gcaatatcta ccgcgccgtc cacagaaatg ccgatatccg cagcgcgtaa
     7201 ggcgggcgca tcattaatac catcgcccat aaagccaacc acatgccctt cgcgcttcag
     7261 taaggtcaca atgcgttctt tatgcatcgg cgtcagacga gcaaacagcg tggtgcgctg
     7321 tgcgagattt gccagttcgt cgtcagatag cgtttcaata tcactaccaa tgaccacctc
     7381 tcccgcgtcc agccccactt catggcacac tttcgctgca actaactcac tgtcgccagt
     7441 aaggattttt acggtaattc cactcgcttt taatgccttc agtgccggag cagttgtctc
     7501 ttttggcgga tcaaggaaag caatatatcc ttcgaggatc aggtcggatt catccgcccg
     7561 ctggtaatct ccttcacgcg ctggcaggta tttcgtcgcc accgcaacca cgcgcagccc
     7621 ctgacgattc agcgtatcag taacccgctt aatcttacgc agcatgatgt catcgagcgg
     7681 cacaatctcg ccattgtgac gcacctgcga acacacattg aggatttcct gcaatgcacc
     7741 tttgcaaacc agctgatggt gctcggtatt ttctgccact accaccgaca tccggcgacg
     7801 ctcgaaatca aacggaatct catcaatttt ttgccaacgg ctggccagcg agcgcgctga
     7861 ctcttcatcc gtaccttcga gcaccgctgt atcgagcagg tttttaagtc cggtctgata
     7921 atgactgttc aaccacgcgc tatgcagcac gcgttcgctg gttttaccgg agatatcggt
     7981 atgattctcc agcacaattt tgtcctgtgt cagggtgccg gttttatcag tgcacagaat
     8041 atccattgcg ccaaagttct gaatagcatc cagatgtttg acgatcactt tctgtttcga
     8101 cagctttact gcgccgcgcg ccagcgtcga ggtaacaatc atcggcaaca tttccggcgt
     8161 taagcctacc gcgaccgaaa gcgcaaacag tgccgcttcc caccagtcgc ctttggtgta
     8221 accgttgata agcaacacca ccggagccat caccagcata aagcgaatca gcagcatact
     8281 gacgcggctg atcccctgct gaaaggcatt cggctcgctt tcctgctcac taacacgccc
     8341 cgccagttga ccaaaccagg tgttggctcc tgtagcaatc accattgctt gtgccgtgcc
     8401 gctcaccacg gtggtgccca taaaacacag ggtgtcgcac tccagcggat tgctgtgctc
     8461 cggctggcga gtggtagcgg ccttttccac gggcagagac tcaccggtta acgacgcctg
     8521 agcgacgaac agatcccgcg cctgcaagat acgtaaatct gccgggatca tatctcccgc
     8581 cgccagtttt ataatatcgc cgggcaccag ctggtcgatc gggatctcca gccagccatt
     8641 ttcgcctttg tcgttaatta cgcgcagcac cgtcgcggta ttgctgacca tcgctttcag
     8701 ggcatctgcc gctttagtgg aacgtgcttc ctgaataaag ttcagcaacg ttgaaatagc
     8761 gaccattagc gcgataacgc ccgcggcaaa taaatcttcc gtggcgtaag aaatagcgcc
     8821 gagaatggtg agtaaaatat taaaggggtt gcgatagcag acccataaat gtacccacca
     8881 cggcgacggt tgttgtgcgg gtaatttatt ttcaccatgt tgttcgcggg cagattccac
     8941 ttccgcctga tttaaccctt ccggatgggt gtcgaacgtt ttccataatt cttcttcggg
     9001 catcaccgcc attttcaggc aatgcgcact taaggacggc gggaccgccg tattcactgt
     9061 ctgctgcgcg ccaggcaatg gatcgcgatg aaccagacgg gaaggtaaat ggcgaatgag
     9121 ccgggtaaaa atttctttaa acataaggag tccctccgca ctgtctgaaa cagtacagaa
     9181 atgtcttcag gcgtggcgaa gccttacctg aaggtaataa agctgagcag ggacgttaaa
     9241 acgtcacggc cttacgcatc cggtaacgcc gtgaaaagac gggcttaccg gaaaagtttt
     9301 aatctccgtc gagggagagg cgtgggatca ggttccatat aacctccggt aagtgaaaaa
     9361 agcgccgcaa ataataagga gaatccaata atattcgtag catctatggc gatataatac
     9421 ctgctggcaa ctaaaccaac gataaaccag actttaccca ttgctgaatg cacgggtaaa
     9481 gttaggctca aataattaaa caacacgtta caggacaaca ggatgcaaaa tcggctgacc
     9541 atcaaagaca tcgcacgctt aagcggcgtg gggaaatcta cagtttcccg ggtgctgaat
     9601 aacgaaagcg gcgtgagcca gcgcacccgc gagcgtgttg aagcagtgat gaatcagcat
     9661 ggattttccc cttcccgctc tgcgcgcgct atgcgtgggc aaagcgataa agtggtcgcc
     9721 atcattgtta cccgtctgga ttcgttgtca gaaaatctcg ccgttcaaac catgctgcca
     9781 gcgttctatg aacaaggtta cgacccaatc atgatggaaa gtcagttttc cccacaatta
     9841 gttgccgaac atttgggggt gctgaaacgg cgtaatatcg acggcgtagt gctgttcggt
     9901 tttactggca taacagaaga aatgttagcc cactggcagt catcgctggt tctgctggcg
     9961 cgtgacgcaa aaggctttgc ttcggtctgt tatgacgacg aaggggcaat caagatcctg
    10021 atgcaacggc tgtatgacca ggggcatcgt aatatcagtt atctcggcgt gccgcatagt
    10081 gacgtgacaa ccggtaagcg acgtcacgaa gcctacctgg cgttctgcaa agcgcataaa
    10141 ctgcatcccg ttgccgctct gccagggctt gctatgaagc aaggctatga gaacgtggca
    10201 aaagtgatta cgcctgaaac tactgcgtta ctgtgcgcaa ccgacacgct ggcacttggc
    10261 gcaagtaaat acctgcaaga gcaacgcatc gacaccttgc aactggcgag cgtcggtaat
    10321 acaccgttaa tgaaattcct ccatccggag atcgtcaccg tagatcccgg ttacgccgaa
    10381 gctggacgcc aggcggcctg ccaattgatc gcacaggtaa ccgggcgcag cgaaccgcaa
    10441 caaatcatca tccccgccac cctgtcctga tcgtttcctg aacgataaat tgtgatcttc
    10501 gctgcgtttc gggaacgttc ccgtttttaa atttttccgc gcaatatatt ctgcagccaa
    10561 ccaaaaatgt catctgccat ggggctttat gatgagcaaa ataaaccaaa cggatatcga
    10621 tcggttgatt gaactggtcg gcgggcgcgg caatattgcg acggtgagcc actgtattac
    10681 tcgcctgcgc tttgtcctca accaaccggc caatgccaga ccgaaagaaa ttgagcaact
    10741 ccccatggtg aaaggctgtt tcaccaatgc cgggcaattt caggtggtga ttggcaccaa
    10801 cgtgggtgat tactatcaag cactgatagc gtcaaccgga caggcgcagg ttgataaaga
    10861 gcaggtaaaa aaagccgccc ggcagaatat gaaatggcat gagcagttga tctctcattt
    10921 cgcggagatc ttcttcccgt tgctgcccgc gttgattagc ggtggtttga tcctcggttt
    10981 tcgcaatgtg atcggcgatt tgcccatgag caacggtcaa acgctggcgc aaatgtaccc
    11041 ttccctgcaa acgatctacg attttctgtg gttgatcggt gaagcgatct tcttctacct
    11101 gccggtcggt atttgctggt cagcggtgaa aaaaatgggc ggcacgccga tccttggtat
    11161 cgtgcttggc gtgacactgg tttctccaca gctgatgaac gcttatctgc tcgggcagca
    11221 gctgccggaa gtgtgggact ttggcatgtt cagcatcgcc aaagtaggct atcaggcgca
    11281 ggtgatcccg gcactgttag ccggactggc gctgggcgtt attgaaactc gccttaaacg
    11341 catcgtgccg gattacctct atctggtggt ggtacccgtc tgttcgctga tcctcgcggt
    11401 gttcctcgcc catgcgctga ttggtccgtt tggtcgcatg attggcgatg gcgttgcctt
    11461 tgcggtacgt cacctgatga ccggcagctt tgctccaatt ggcgcggcat tgtttggctt
    11521 cctgtacgcc ccgctggtga tcaccggggt acaccagacc acgcttgcta ttgatttgca
    11581 gatgattcaa agcatgggtg gtacgccagt gtggccgctg attgcgctgt cgaatatcgc
    11641 tcagggctcc gccgtgatag gcattatcat ttccagccgc aagcacaatg aacgcgagat
    11701 ctccgtgcct gcggcgatct ccgcctggct tggggtcact gagcctgcaa tgtacggcat
    11761 caacctgaaa tatcgcttcc cgatgctgtg cgcgatgatt ggttctggtc tggcaggatt
    11821 gctatgcggc ctgaacggcg ttatggcgaa tggcatcggc gtagacggcc taccgggaat
    11881 tctctcgatt caaccgagct actggcaggt gtttgcgctg gcaatggcta tcgccatcat
    11941 catcccgatt gtactcacct cgtttatcta tcagcggaaa taccgcctgg gcacgctgga
    12001 tattgtttaa ttttcttcgg ggcgcaattg cgccccctcg catttgcagg aataacgtaa
    12061 tgactaatct tccccactgg tggcaaaacg gcgttatcta ccagatttat ccaaagagtt
    12121 ttcaggacac cacgggtagc ggcaccggcg atttacgtgg cgttatacaa cgcctggact
    12181 atctgcataa actgggcgtt gatgccatct ggctaacccc cttttatgtc tctccccagg
    12241 tcgataacgg ttacgacgta gcgaactata cggcgattga tcccacctac ggcacgctgg
    12301 acgattttga cgaactggtg acgcaggcaa aatcgcgcgg gattcgtatc attctcgata
    12361 tggtgtttaa ccatacctct acccaacatg cctggtttcg cgaggcgctg aacaaagaaa
    12421 gcccttaccg ccagttttat atctggcgcg atggcgaacc agaaacacca ccgaacaact
    12481 ggcgttcaaa atttggcggt agtgcgtggc gctggcatgc ggaaagcgaa cagtactatt
    12541 tgcatctctt tgcaccagaa caggcggatc tcaactggga gaatccagcg gtacgcgcag
    12601 agctgaaaaa agtctgtgag ttctgggccg atcgtggggt tgacgggttg cgcctggatg
    12661 tggtgaatct gatctccaaa gacccgcgtt tccctgaaga cctggatggc gacgggcgtc
    12721 gcttctacac cgacggacca cgagcacacg agtttttgca cgagatgaac cgcgatgtgt
    12781 ttacgccacg cgggttaatg accgtaggtg aaatgtcctc caccagcctt gagcattgcc
    12841 agcgatacgc ggcactgaca ggcagtgaat tatcgatgac ctttaatttt catcacctga
    12901 aggtcgatta tcccggtggt gaaaaatgga cgctggctaa acctgacttt gtggcgttga
    12961 aaacattgtt ccgccactgg caacaaggaa tgcacaacgt agcatggaat gccttgttct
    13021 ggtgtaacca cgatcagccg cgcattgttt ctcgctttgg tgatgaaggt gaataccgcg
    13081 tgcctgcggc aaaaatgctg gcgatggtgc tgcatggcat gcagggaacg ccgtatatct
    13141 accagggcga agagattggc atgaccaacc cgcatttcac gcgcattact gactatcgcg
    13201 acgtggagag cctcaatatg tttgccgagc tacgcaacga tgggcgtgat gccgacgagt
    13261 tattggcaat cctcgccagc aaatcccgtg acaacagtcg cacacccatg caatggagca
    13321 acggcgataa tgccgggttt acggctggcg aaccgtggat tggcttgggc gataactatc
    13381 aacaaatcaa cgtagaagcc gcgctggccg atgattcctc ggtgttttac acctaccaaa
    13441 agttaatcgc actgcgtaag caggaagcca tcctgacatg gggcaattac caggatctgc
    13501 tgccaaacag ccctgtattg tggtgctatc gccgtgaatg gaaggggcaa accttgctgg
    13561 tcattgccaa ccttagccgt gagatccaac cctggcagcc agggcaaatg cgcggcaact
    13621 ggcagcttgt gatgcataac tacgaagaag cctcaccaca accctgtgcc atgactttac
    13681 ggccttttga ggctgtctgg tggttacaga agtaagtatc cgctaagccc cgggaatgcc
    13741 tgtcattccg gggttttgtc tgtcagcatg ttgtttttgt tgatttttca accagcaaat
    13801 tcattaaaaa atttacataa cgctgtagcg cccgtcatcc gtacgctctg ctttttactt
    13861 tgagctacat caaaaaaagt tcaaacatcc ttgatgcaaa gcactatata tagactttaa
    13921 aatgcgtccc aacccaatat gttgtattaa tcgactataa ttgctactac agctccccac
    13981 gaaaaaggtg cggcgttgtg gataagcgga tggcgattgc ggaaagcacc ggaaaacgaa
    14041 acgaaaaaac cggaaaacgc ctttcccaat ttctgtggat aacctgttct taaaaatatg
    14101 gagcgatcat gacaccgcat gtgatgaaac gagacggctg caaagtgccg tttaaatcag
    14161 agcgcatcaa agaagcgatt ctgcgtgcag ctaaagcagc ggaagtcgat gatgccgatt
    14221 attgcgccac tgttgccgcg gttgtcagcg agcagatgca gggccgcaac caggtggata
    14281 tcaacgagat ccagaccgca gttgaaaacc agctgatgtc gggtccatac aaacaactgg
    14341 ctcgtgctta catcgagtac cgtcacgatc gcgacattga acgtgaaaaa cgcggtcgcc
    14401 tgaaccagga gatccgtggt ctggtcgagc agaccaacgc ctcgttactc aacgaaaacg
    14461 ccaacaaaga cagcaaggtg attccaaccc agcgcgacct gctggccggg atcgtggcta
    14521 aacactatgc acgccagcac ctgctgccgc gtgacgtggt gcaggcacat gagcgtggcg
    14581 atattcacta tcacgatctc gattactcgc cgttcttccc gatgttcaac tgcatgttga
    14641 tcgacctgaa aggcatgctg acccagggct ttaaaatggg gaacgccgag attgaaccgc
    14701 cgaagtcgat ctctacggca accgcggtaa ctgcgcagat tattgctcag gttgccagcc
    14761 atatttatgg cggcaccacc attaaccgta tcgatgaagt gctggcaccg tttgtcaccg
    14821 ccagctacaa caaacatcgc aagaccgcag aagagtggag catcccggac gcagaaggct
    14881 acgctaactc tcgtaccatt aaagagtgct acgatgcctt ccagtcgctg gagtacgaag
    14941 taaacaccct gcataccgcc aacggtcaaa cgccgtttgt aacttttggt tttggtctgg
    15001 gcaccagctg ggaatcgcgc ctgattcagg aatctatcct gcgtaaccgt atcgcaggtc
    15061 tcggtaaaaa ccgtaaaact gcggtgttcc cgaaactggt gtttgcgatt cgcgatggtc
    15121 tgaaccataa aaaaggcgat ccgaactacg acatcaaaca gctggcgctg gagtgcgcaa
    15181 gcaagcgcat gtatccggat atcctgaact acgatcaggt agtgaaagtc accggttcgt
    15241 ttaaaacccc gatgggctgc cgcagcttcc tcggcgtgtg ggaaaatgaa aacggcgagc
    15301 agatccacga tggtcgtaac aacctcggcg tgatcagcct gaacctgccg cgtattgctc
    15361 tggaagcaaa aggcgatgaa gccaccttct ggaagctgct ggatgaacgt ctggtgctgg
    15421 cacgtaaggc gctgatgacc cgtatcgctc gtctcgaagg cgtgaaagcg cgcgtggccc
    15481 cgatcctcta tatggaaggt gcttgtggcg tgcgtctcaa tgctgacgat gatgtttctg
    15541 aaatcttcaa aaacggtcgt gcgtctattt cgctgggtta catcggcatc cacgaaacca
    15601 ttaacgcgct gttcggcggc gagcatgttt acgacaacga gcagcttcgc gcgaaaggta
    15661 tcgccattgt tgaacgtctg cgtcaggcag tggataagtg gaaagaagaa acgggttatg
    15721 gtttcagtct ctacagcacg ccgagtgaaa acctgtgcga ccgcttctgc cgtctcgata
    15781 ctgctgagtt tggcgtggtg ccgggcgtga ccgataaagg ttactacacc aacagtttcc
    15841 acctcgatgt ggagaagaag gtgaacccgt acgacaagat cgactttgaa gcgccttacc
    15901 cgccgctggc gaacggtggt ttcatttgct acggcgagta tccaaacatt cagcacaacc
    15961 tgaaggcgct ggaagatgtc tgggattaca gctatcagca tgtaccgtat tacggcacca
    16021 atacaccgat tgatgagtgc tacgagtgtg gctttaccgg tgagttcgag tgcaccagca
    16081 aaggtttcac ttgcccgaaa tgtggtaacc atgacgcctc ccgtgtgtcg gtgactcgcc
    16141 gcgtgtgcgg atatttaggt agcccggatg cacgtccatt taacgccggt aagcaggaag
    16201 aagttaagcg ccgcgttaaa catttgggga atgggcagat aggttaatct tcactctacg
    16261 ttaagcaaat gccggatgcg gcgtaaacgc cttatccggc ctacatggca acaacgattg
    16321 taggtctgat aagacgcgac agcgtcgcat caggcgttga ttaccggatg catcctatac
    16381 gcggactgat aagccaggcg aaacaatgaa ttatcatcag tactatcctg tcgacatcgt
    16441 caacggcccc ggcactcgct gcaccctgtt tgtctccggg tgtgttcatg aatgccccgg
    16501 ttgctataac aaaagcacat ggcgggtaaa ttccggtcag acatttacta aagcaatgga
    16561 agaccagatc attaacgatc tgaatgacac tcgcatcaaa cgccagggta tttccctctc
    16621 cggcggcgat ccgctgcatc cgcaaaacgt gccggatatt ctgaaactgg tacaacgcat
    16681 ccgcgccgag tgtccgggta aagacatctg ggtgtggaca ggctataaac tcgacgaact
    16741 caacgctgcg caaatgcagg ttgttgattt gattaacgtg ctggtcgacg gcaaatttgt
    16801 gcaggattta aaagaccctt ccctgatctg gcgcggcagc agcaatcagg tggtgcatca
    16861 tttgcgatga taagttccct gttacctgag tgcaggcaac agggaaatga ggaattaacg
    16921 atacttctgg tgataggcgt tgcgggtcgt tttcagttgc tctgcggcag cctgcgcttc
    16981 ttttacttta ccttcatttg ccagcttcag cgcgtcgtca atctgaccga ccagaatatc
    17041 gaaaccgtgg cggaagtctt tcatttccgg gctgtccggt gatttgcctt caagcttcgg
    17101 cggcgtcgct ttttgcgcat ccagcgctgc ggcgcgcatc ttcgttaacg cgtctttgac
    17161 ttgcgccgcg ttatcggctt tttcgaccac ttttaaattg tcgttgaggg tttccatatt
    17221 gtcttcaaga tcagcggcaa acgacgccga actgaatacc aacgaggaga ctgcaagaat
    17281 agctaacagg cttttacgca ttgctcactt ccttttttat tatgaagatt tggcgcggca
    17341 ggtcgcgcca gttttttact gcccggcgat tttcatctcc gacaacagca cagaaccaca
    17401 ctgtatatta ctgcgtgttt caatatcgtt accgacggtg acaatattgc gccacatatc
    17461 ttttaaatta cctgcgatgg tgatttcgct caccggatac tgaatttcgc cattctctac
    17521 ccagaagccc gcagcaccac gggaataatc accggtaatg gcactcacgc cctggcccat
    17581 cagctccgtc accaccagcc cggtgcccat ctctttgagc atttgttcga agcttagccc
    17641 ttgtccggca atccgccagt tgtgaatacc gcccgcatgt ccggtgcttt tcagccccag
    17701 tttccgcgcc gagtagctgg tcagcagcca ctgagtcagg atgccatctt taataatatc
    17761 gcgacgctcg gtgcgcacac cttcgctgtc gaatggcgtc gacgccagcc ctttcagcag
    17821 atgcggatgc tcttcaatgg tcagccagtc cggcagaatt tgtttaccca gcgaatccag
    17881 cagaaaggta gatttacggt aaaccgagcc acccgctatc gcccccacca gatggccaaa
    17941 aagcccggtt gccacttcat tggcaaaaat gaccggcgct ttcatggtgg agagtttacg
    18001 cggtgacagg cgcgataaag tacggcgagc acagtcggcc ccaacccact ctggcgtttg
    18061 cagatcgctc atcgcacgac caatggtgta ggcgtaatca cgctccatat cgccattttc
    18121 ttcggcaatt acacagctgg agagcgaatg acgcgtagag cagtaaccct gcaacatgcc
    18181 gtggctgttg ccaaaaactt tgacaccgta gtggctgtta aagctgccac cttcggtatt
    18241 ggtgatacgt ttgtccgcct gtaatgctgc ctgttctgcg cgggccgcca gttcaatggc
    18301 ttcatccggg gaaacttccg cagggtggaa caaatcgaga tccggtgcct caaaggccag
    18361 cagctctttg tctgccacgc cggcacaggg atctggcgag gtataacggg caatatccag
    18421 cgccgcctgt acggtgcggg caatggcctg cgggcttaaa tcggtggatg atgcgctacc
    18481 tttgcggttc tggtgataaa cagtgatccc cagcgcgcca tcgctattga attcaacatt
    18541 ctccacttca ccataacgcg tgcttacgct aatgccggtg gtcttgctga cggcaacttc
    18601 cgcaccgtcc gatttgcctg aggccaactc cagcgcagtg gaaactgctt cttccagaat
    18661 cttgcgctgc gcttcaactt gagagattac tttcattgca agtgccataa tgtagagagt
    18721 ttctctgaag tctaacagag aaccgttttt cagtgcgcat cttaactggt aacattagcc
    18781 tcttttttta aggagcctga gatgactaag cagcccgaag actggctcga cgacgttccc
    18841 ggtgatgaca tcgaagacga agacgatgaa attatctggg tcagtaaaag tgaaattaaa
    18901 cgtgatgccg aggagctaaa acgccttggc gcggaaattg ttgatctggg gaaaaacgcg
    18961 ctggataaga tcccgttaga tgcggatctg cgcgcggcta ttgagctcgc ccagcgtatt
    19021 aagatggaag gtcgccgccg ccagttgcag ctcatcggta agatgctgcg ccagcgcgac
    19081 gtagagccta ttcgtcaggc gctggataag ctgaaaaacc gtcacaacca gcaggtggtg
    19141 ctgttccata aactggaaaa cctgcgcgat cgtctgatcg atcaaggtga tgacgccatc
    19201 gctgaagtat tgaatctgtg gcccgatgcc gatcgtcagc aactgcgtac actgatccgc
    19261 aacgcgaaga aagagaaaga agggaataag ccgccgaaat ccgcacgcca gattttccag
    19321 tatctgcgcg aactggcgga gaatgaaggg taattttcgt tgttgatgtg agtatggttt
    19381 gccggatgcg gtgcacctgt aggcctgata agacgcatca agcgtcgcat caggcactat
    19441 gctcaaatgc cggatgcggc gtgaacgcct tatccggcct acgcacacta tatcaggcta
    19501 aggccgaatt actgcgcggc ttccgccttc ttcgccagac catccagcag tttctgatgg
    19561 atcccaccaa aaccgccgtt gctcatcacc agaatatggt cgccaggctg agcggttttc
    19621 accaccatat ctgccagcgt atccacatcg ccactccagt gtgcaggctg aacgcaggct
    19681 tctgccactt ctgccacctg ccacggaata tgcgccggtt gcagcaggaa gacttcatcg
    19741 gcacgaccta atgaaggtgc cagatcgtct ttgcagatcc ccattttcat ggtattcgag
    19801 cgcggttcca gcacagcaat aatgcgcgcc gtaccaccaa ctttgccacg cagcgccgca
    19861 agcgttgcca gaatcgccgt cgggtgatgg gcaaaatcgt catataccgt cacaccattc
    19921 gcttcaccac gcaactccag acggcgacga gcattaataa acgaacccag cgcgttagcg
    19981 gcatctgccg gcgctacacc aacatggcga gccgctgcaa tcgccatcag gccattgtgc
    20041 atattatgtt cgcctaccag cgaccatttc acttcgccca ctttttcgcc atccagcaaa
    20101 acttcccatt cggaagcatc ggtggtcagc tttttcgcct gccagtgacc ctgctcaccc
    20161 accagctcct gctcgctcca gcagcccatc gccatggtct gtttcaggtt gatgtcattt
    20221 tccggccaga taatacggcc ctgccccgga acgatacgca ccagatggtg gaactgtttc
    20281 tggatcgctt tcaggtcgtc aaagatatcg gcgtgatcga actcaaggtt gttgaggatc
    20341 agcgtacgcg ggcagtaatg gacaaattta gagcgtttgt cgaagaaggc gcagtcatac
    20401 tcatccgctt cgataacaaa gaagtcgctt tcgcccagac gcgccgaaac ctcaaagttc
    20461 cccggcacac cgccgatcac aaatcccggt ttgtaaccgc actgttccag aatccaggtc
    20521 gccattcccg cggtggtagt tttgccgtgt gtaccggcaa cggccagcac ccagcggtcg
    20581 cgcagcacaa aatcgtgcag ccactgtgga cctgacatat aagggatgtt tttttccagt
    20641 accgcttcca cacacggatt tccacgggtc atggcgttgc caataatcac cagatccggc
    20701 tgcggatcga gctggctggc atcgtaaccc tgaatcagct caatgccttg cttctcaagt
    20761 aaggtgctca tcggcggata cacattggcg tccgaacccg ttacttcatg gcctaactgg
    20821 cgcgccagca tcgccagacc gcccataaat gtgccacaaa ttcctaaaat atgaatgcgc
    20881 atacgtcact atcctttttt aatctgccgc tcattttacg catatgtcgg gcaagtgaga
    20941 aacgcatttc aggataatcc gcaatttgct ggcgcgattc acctgagcgc aacattgaga
    21001 ttatttgtta agattgttgc ggtcgcttta ctccataaac attgcaggga aagttttatg
    21061 aaaacgttag gtgaatttat tgtcgaaaag cagcacgagt tttctcatgc taccggtgag
    21121 ctcactgctt tgctgtcggc aataaaactg ggcgccaaga ttatccatcg cgatatcaac
    21181 aaagcaggac tggttgatat cctgggtgcc agcggtgctg agaacgtgca gggcgaggtt
    21241 cagcagaaac tcgacttgtt cgctaatgaa aaactgaaag ccgcactgaa agcacgcgat
    21301 atcgttgcgg gcattgcctc tgaagaagaa gatgagattg tcgtctttga aggctgtgaa
    21361 cacgcaaaat acgtggtgct gatggacccc ctggatggct cgtccaacat cgatgttaac
    21421 gtctctgtcg gtaccatttt ctccatctac cgccgcgtta cgcctgttgg cacgccggta
    21481 acggaagaag atttcctcca gcctggtaac aaacaggttg cggcaggtta cgtggtatac
    21541 ggctcctcta ccatgctggt ttacaccacc ggatgcggtg ttcacgcctt tacttacgat
    21601 ccttcgctgg gcgttttctg cctgtgccag gaacggatgc gcttcccgga gaaaggcaaa
    21661 acctactcca tcaacgaagg aaactacatt aagtttccga acggggtgaa gaagtacatt
    21721 aaattctgcc aggaagaaga taaatccacc aaccgcccgt atacctcacg ttatatcggt
    21781 tcactggtcg cggatttcca ccgtaacctg ctgaaaggcg gtatttatct ctacccaagc
    21841 accgccagcc acccggacgg caaactgcgt ttgctgtatg agtgcaaccc gatggcattc
    21901 ctggcggaac aagcgggcgg taaagcgagc gatggcaaag agcgtattct ggatatcatc
    21961 ccggaaaccc tgcaccagcg ccgttcattc tttgtcggca acgaccatat ggttgaagat
    22021 gtcgaacgct ttatccgtga attcccggac gcgtaatctc ctgcgcctct ccacgcggag
    22081 aggcgttttt atcgctgcgc aatgttgact cttgtcactg gcgaactctg acgattctcc
    22141 cacagcaccg ttaatccacg ctgtaatgct ataaaaataa acaacaaaat accgatggcg
    22201 attttcgtcc accaggaact cagcgtgcca tcaaagttta tgtaagtctg aatcagtccc
    22261 tgaatcgcca cgccaaaaag cgtccctaat accgttccaa cgccaccgct caaaagcgta
    22321 ccgccaatta ccactgaggc gatagcatcc agttccacac ctacgcccgc cagcgcatat
    22381 ccggcctggg tataaatcga gaagacaatc cccgccagcg ttgccagtcc ggtggagagc
    22441 atataaatgc gaatagtggt gctgcgagtg gaaatcccca tcagattcgc cgacgttgcg
    22501 ttgccgccaa tggcgtatac ctgattacca aaacgggtac gatgcgcgag gaatatgccg
    22561 ataaccacca ccgccaacat cagcagtccc atcgcactta agcgaccgcc gccagggatt
    22621 ttccacgcaa ggcttgagag cgtgtcataa atgggatggt ttatcgggat cgactcttcc
    22681 gaaacgagat agctgacgcc gcgcaaaaag aacatccccg ccagggtaat gataaatgct
    22741 gggatcttca gggcgtcgat cagaagcccc ataaatgcgc cgaaggcaca gcccatcacc
    22801 agcactagcg gaaacgccag cagcggcgag aggccgaaat cgccaatcac ttttgccaga
    22861 aacacgccag taaaggcgat caccgaaccg acggagagat cgatcccacc ggagaggatc
    22921 acaaaggtca tgccaacggc aatgatccca agaaaggcgt tatcggtcag gatattgcag
    22981 atcactcttg tggaagcaaa accaggaaac tgcgtcaggc agtagagata gcccagcaca
    23041 aagacgccga tggtgatcat aagcggcaag ttacgtttta tcacggctac gcactccttt
    23101 aatcagactg ataaagcgtt gcgactgaac aatcagcaca caaagcacca ccaccgcttt
    23161 tacgacctgg ttcatctctg gcggaaagcc agaaagtaaa attccggtat tcattccctg
    23221 aataatcagc gcccccacca ccgaaagcag caggttaaag cgcccgccca tcagcgatcc
    23281 gccgccaatc accaccgcaa gaatggcgtc cagctccagc cataatccgg cgttgttggc
    23341 atcggcaccg cgaatatccg ccgccacgat aatgcccgca atcgccgcac acagcccgct
    23401 caacacataa gtgagcatga cgatgattcg tgtgttgact ccggcatttt tggccgcccg
    23461 aatgttgata ccaacggctt cgataaacat ccccagtgcc gttttgcggg tcaacagcca
    23521 gaacaggaga agcgtcagca ccgcgataat gaccggcgtt ggcaggaaca acagcgatcc
    23581 gctgccaaac catgagagat ccggcgagtt aaacgtgacg atctgtccgg aggtgatcag
    23641 ttgcgccacg ccgcgcccgg cgaccatcag gatcagggtg gcgacaaacg gctgaatttt
    23701 gaggatcgct accagtatgc cgttccacaa tcccgccagg atgccagtgc ccagggcgct
    23761 taacaaaaca atcggcaggc tgaatcccgc gaccgtcatc gcagccgttg tggctccggc
    23821 tatagccatc accgccccca cggagagatc aatcgcacct gttgcgatca ccagcgtcat
    23881 accaatcgcc agtaacgcaa cgggagctgc acggttaaga atgtcgatag ggctaccgaa
    23941 caaacgccca tcctgaagca ctacctgcca gaaatgcggg gctaccaggc tgtcgaccag
    24001 cagcaccagc aatagtgcta ccagctgcgg catccccgtc ggccagcgaa agcgcctttt
    24061 tggtgtagtg gtgtccggaa gagattgagg catcacactg ttctccttac gccgcaatgg
    24121 cgttcatgat cgccggaacg gaaagctctg ccagcgggat ctccgccacc tgtttgcgat
    24181 cgcgcatgat gatcacccga tcggcatagc ccaccagctc ttccagttcg gaggagatca
    24241 ccagcagcgc cagaccatcg gcgcatagcg tttcaatcag gcggatgatc tcggcgtggg
    24301 caccaacatc aatgccgcgg gttggctcat caaggatcag aaattgcggt cgggtcagta
    24361 gccaacgtga aagcaatact ttttgctgat tgccgccgga gagaaattca atcggttgtt
    24421 cagttgaagg tgtgcgaatg ccaagctgac ggataaagcg ttcggcaatc tcttgctgtt
    24481 ctttacggga aataggtcgt agccagccgc gctgggcctg gagagcgagg atgatatttt
    24541 cccgcaccga ggcagcagcg atgatgccat cggttttcct gtcttccggg cagaagccga
    24601 tgcccagtac cgaagcctga tgtggcgatc gcaggttttg cggtttgcct ttgatcaacg
    24661 ccgtgccgct gtcagcaggt ttgataccga agatcacttc ggcggtttcg gtacgtcctg
    24721 atcccagcaa tcctgccagg ccgacgatct cgccggggcg tacttcgaga tcaaacggtg
    24781 cgatcgttcc ttttttgccg taatttttga acgcggcaat gggtttgtgt cgctcaacaa
    24841 tgttcgcccg gcacgctgta gcgcgtgggt atccagctcg cgccccagca tcatttttac
    24901 cagttcgatc tgcggtagct cgcgcgtttc ccgacagcca acaaaactgc cgttgcgtaa
    24961 gacggtgatc cgatcgctaa cctgatagac ctgatcgaga aagtgagtga caaagatcag
    25021 gctgacgccg cgatcgcgca actgacgcat caggccaaac agtaattcta cttcctgagt
    25081 gtcgagactg gcggtgggtt catcgaggat cagcactttg gcggagagat cgatagcccg
    25141 gcaaatggcg acgatttgct gcatcgcgac tgaaaagcgg ttaagcggtt cacgcacgtc
    25201 gagggagaaa ccgtaagatg tcatcagttc ggtggcacgc ttttccatct ctttgcggcg
    25261 tagaaagccg aaacgtttgg gttcgcgacc tatgaataga ttatcagcga ccgacatatt
    25321 gggtagcagg ttgacttcct gataaacggt tccaatgccg agttgttgtg catgggcggt
    25381 atttttcggt gagatagcct ggccttccag ccagatggtg ccgcgatcgg cgtggtatac
    25441 accggttaat gctttgatta gcgtcgattt tcccgccccg ttttcaccga gcagcgccat
    25501 gatttcgcca cggcgcaggc tgaaatcgac gttgtctaac gctttgacgc cggggaaaaa
    25561 tttacttaat ccttcggtgc ggaggatttc ctggtgttgg tcggtcgtca tggtttcccc
    25621 cggtgtaaag tgtgctccca gtaggcatga taagacgcgg taagcgtcgc atcaggcttc
    25681 agtgcaccat cgccggatgc ggcgcgagcg ccttatccgg cctacgaatc ggcatcaggc
    25741 atagcaaccc tcaatacccc atatttttct tcttctctaa ctcttctttt gcggtatcag
    25801 gcagataaag ggtggatttg gtcagggtca gcttttcagg catggtgccg tcttttttgt
    25861 atttctccag cgcgtcgaag gcgggacctg ccatattcgg cgtcagttca acactggcgt
    25921 tcgcttcgcc atcaatcatc gctttgtaga tatccggtac gccgtcgata gaacctgtca
    25981 gaatatcttt gcctggtttc aggcccgctt ctttaattgc ctgaattgca ccaatcacca
    26041 tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc gttgttttcc gctttgataa
    26101 agctctccat gacttctttg cctttactgc gggtgaagtc acctgactgc gagcggataa
    26161 ttttgatatt tggcgcattc ttaatggctt cggcaaagcc tttcttacgg tcaatggcga
    26221 cgctggcccc aacagtgccc tgcagctcca ccacgttgca tggtttgcca ttcacttctt
    26281 ttaccagcca gtcaccaatc aacttgcctt cgagaatgtt gtcggcagtg acggtggtca
    26341 tatagagaga tttgtctttc acatcaatag aacgatcgag caagaagacc gggatttcgg
    26401 catctttcgc ctcttttaat accggttccc aaccagtcgc aaccaccgga gcgataaaga
    26461 tcgcatccac cccttgcgcg acgaaggaac gtaccgcttt aatctggttt tcctgctttt
    26521 gctgaccatc ggcaattttc agcgtaattc cgcgtttttc ggcctcactt ttcgccacat
    26581 tggtttctgc ggcgcgccag ccggattccg agccgacctg cgaaaatcct acggttaatg
    26641 gagcggccaa cgccatagac gacatggctg ccgagactgc agagactaca agtaagcgtt
    26701 tccacatagg gttgtcctcg tcggagtaat tattattggt gaaaagatgt tcgcgaaaaa
    26761 actatagaca attcgttatg taacggattg cgttacatca catttcaaaa aagtaaataa
    26821 aacgttaatc acaagtttgt aatcgctttc atctcactat gaaaaaagcg gctacggtta
    26881 tggattttcc tgctctgtat accgtcttaa aactggtgaa aaaggaaaat gaagacgaaa
    26941 acaagcgaag acattcggcg cgagttggct ataatactcg gcacttgttt gccacatatt
    27001 tttaaaggaa acagacatga gcttactcaa cgtccctgcg ggtaaagatc tgccggaaga
    27061 catctacgtt gttattgaga tcccggctaa cgcagatccg atcaaatacg aaatcgacaa
    27121 agagagcggc gcactgttcg ttgaccgctt catgtccacc gcgatgttct atccatgcaa
    27181 ctacggttac atcaaccaca ccctgtctct ggacggtgac ccggttgacg tactggtccc
    27241 gactccgtac ccgctgcagc cgggttctgt gatccgttgc cgtccggttg gcgttctgaa
    27301 aatgaccgac gaagccggtg aagatgcgaa actgattgct gttccgcaca ccaagctgag
    27361 caaagaatac gatcacatta aagacgttaa cgatctgcca gagctgctga aagcgcagat
    27421 cgctcacttc ttcgagcact acaaagacct cgaaaaaggc aagtgggtga aagttgaagg
    27481 ttgggaaaac gcagaagccg ctaaagctga aatcgttgct tccttcgagc gcgccaagaa
    27541 taaataagtt cttatagcgc aataaccctg aacaccgggc ttcggttagt aagggttttt
    27601 ttatgcccgc gataaataaa ctctctattc caccatcatt attctcagcg gttgcaaggc
    27661 ttgaacggta agaacaagca aacccgacca ccattttgct gttcatagcc acttgctgga
    27721 agttagccga cctcactcat actcaccgtt caaagcgtgc ccccgtttta cccctgcaca
    27781 tttgtgaacg ccagcagagt tttttatgcg attgcagata tggaaagcgg ctcggagaga
    27841 aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga tacatcgcat aaaacaacgc
    27901 cacccgaagg tggcgtgctt ttattattac ttatccctgt ctaaccagtc gccgctttca
    27961 attagcttta atccatcgac gggtcgttga taaacgtaca tccatgcact cccgtacggc
    28021 gtctgaatca actggcgcgc gtattcaccg cccctggtgc gcaaggcatc aagttcggcc
    28081 agcgtggcgt tgtcaatacg ataaacttca ccgtgtactg ttccgttccc cggaactgcg
    28141 cctggatagt ggcccaggct atacaactgg tagttatcga tactgaaatc gcccagtaac
    28201 tgggcattgg tcatccagtg actgttgcct tgtttgtggc gtaaactgcc gtagacaaat
    28261 attcgcattg ctaaaactcg aactgataga gcaaatccag tgcctggtct acaccagaca
    28321 cggcttccag atatagctta ggcatcaggc gataacgtaa cgtgagtgtt gctatagagt
    28381 caaatatacc cacgccgtat ttcacttgca gacctggcaa tacatagccg ctgaccacta
    28441 cctgggagga gtcgcctact ccctgggtgt cgagcgctaa attgcttacg ccaaacgtct
    28501 cgccgatttt acccacaatc tggccacttt gcgcaacccc cagaccaatc agcatcgagg
    28561 tcattgccgc actgtcgctc tgatcgctct ccagcccttg tccacgtagc aaataagaca
    28621 atgcagcttg ttgcgacatc gccgggtcag agaagatctc cgctttcggt tcgtccgcca
    28681 gaccagtgac gcgaactccg gcgattacgt cgtcttctgt agcatccggg ttacgaatag
    28741 cttcaatatt aagatacggt tgatccggcg gaccagagaa cagtaactca cctttacgca
    28801 caatcagatc ctgaccatag gcatggaagc gcccttcagg gatgttgatc tgcccgttca
    28861 ggcccagccc ttgtttgtcc tgaacgacgt taagatcgcc cgtcagccgc gctttcaggc
    28921 caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat caggttactg ttaatcggaa
    28981 tcgacgccgt tttcggctct tccggttgca ggttatcgtt aagcatcacc acatcgctgg
    29041 agacgcctac tgcgctttcc ggcagatcgt gcaccacgat gcgcgcccac gggacatcca
    29101 cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa gacaacatct ggcgatacat
    29161 ccattcgtac catcggcggc acggtgatcc gcactttact gcctttcgcc gttacccgcg
    29221 cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt caggtagatt tcaccctgct
    29281 gggtccgtac tgtaccggca agcgtcgagc gcataccatt aaagttgacc gcaagctggc
    29341 tcggttgcat atcaaacggc ataaagttgc cgtcgatatc cacacccgta acctgaagct
    29401 gaccaaacaa ctgcgggctt tgcacatcac cacccagacg caagttggca ctcaccatcc
    29461 ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat cgcaaggttg aagttacgga
    29521 tattgacgtt gccaccaaga ttacggcggc cttgcggatc ggtcacctgc acctgtccat
    29581 caaactggcc gttattggtc aggcggatgg tccagcccaa ttcggcacgg ttgttacgca
    29641 attccgccgt cagattcagt gtctgaaacg ccacgggcag cgccgcatcg ttgacggttt
    29701 gcgttacctg cacgttacgc cccgaaaggg tgatactgcc ctgcggcagc ccctctttgg
    29761 tggtgtccca ggcaacgtcc gctttacccg tgaagatacc gctggcctga gtggtttctg
    29821 gcataaatgg tttcagcatg gccaggtcga agcggttgag attcaccacc gcacgccctt
    29881 cggcacccgc atcgatagtt tgcggcacgc acagttccgc attcgggtta agccagcagt
    29941 gtggcccgat gctgattttt tgctccttat tgcggtaatc cagcgcaata tcgcgggtca
    30001 gcgaccacgg gccgaccggc gtctggaagc gggtattgct aagagttccc ttccagcgtt
    30061 cttctttgcg atcaaaactt cctgccagat taagctgccc ggagacaggc tcgccctgaa
    30121 tccgcaactg taactcgtgc tgtttttcgc tgcctttggc attcagggtg acgaggttga
    30181 tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc gagtttcccg gcgatctgat
    30241 cggtggattt aatgtcgcct tccacgcgaa cctgcgccac ggaaagttcc tgccagcgca
    30301 ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc caccgtgccg cgtactttca
    30361 ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt atccagcccc ggcgcgttga
    30421 tggtggcatc aagattgaga tcttttaccc ccagctcgcc tttcacttcg gcactgtttg
    30481 gccccagttc cagatgaagc cctgggatca tccactgcat ataactgttg cctttcagcg
    30541 tgccgtcaac gttcactttg ttctgtttga cgttaccggt cagcttcagt tctggcacct
    30601 ccatctgcca ggtgccgccg tacaggctac cgcgggtttt aatcaagcca ttgagtttcg
    30661 acggccactc tgggatctct ttggcggtgt taatgccgtt aagcgttagc tcaccgcgcc
    30721 aactaatggc ctgctgccag tcgagcaacg ccttgagttc agttttccct tccagcgccg
    30781 cgacggtgag tttgtcgaga ttgacctgct gttcattacc tttggcatca agggtaatgg
    30841 tggctggcgg gatctccagt cccttcactg ccgtacgcat agagagcgtg taatcggtca
    30901 ttttgccggt aagtttcagt ttcagatcat ccgcctgata ctgcttctca ccagtgaacg
    30961 gccagtaaat ctgtttgctg ttcacttcca cgttgagcgg caatccggct tccgccagtc
    31021 gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag attaacgcca atctccagct
    31081 gttcgcgcag cgcgccgccc actttcagct tcaccttttc acctttcaac ggctccacgt
    31141 tcagtgtact attgagagtg atgtccaccg gccagttgtc tgacagctgc gccgtgccgc
    31201 tggcgttgac gatcccttga ctggaatcga tatccagggc gtccagttta gtattgccgt
    31261 caatgctgct cactttcagc agcatggtgc tcacggtgat gtccgtgtcg cccgtcacgc
    31321 gcaactgctc gcccttaaac tcttcaatgt tcaggttaag cggcaaatgc acgtcggtca
    31381 tttccggcaa taccgggcga gaaaagagat ctttcagcgt ttcgccgagc ggcttttcct
    31441 ccggctgcgg attttcaatt ttcggttcga ccacttcttc ctgcgccact tccgccactt
    31501 tcggcagagc aatcagcagg cctttcagcg acgtcggttt cagggtcagg gttttctcct
    31561 gccagttcag gccggaggtg aagtccatca ccgataccgt ggtgtcatca atcttgatgt
    31621 tgacgttgtc cagtgccacc cgtgttaggg tgatgggata cggcgtggag agatccagcg
    31681 gaccgctatc ttcctcttct tcaacctgtt cagaaggggg catttttttg ctgtcgatat
    31741 tgacctggat gtctttcagc gccagatcat taatacaaac actgctgttc cacaggcact
    31801 caagcccgac cgccagatgc agattgcctg cttttaccgc cacgcctggc tgctcataac
    31861 gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac cttgccaaca tccagtcctg
    31921 gcacccagcg atccgccgct ttaaatacca gatgcaggcc gctggtggtg cccaccagaa
    31981 acgccaccga tcccagcaac agtaagataa cgataaccac gccgaggctg atttttttcc
    32041 ataaactcat aattctggcc ccagaccgat gtaaaactgt aacccgtgtt cgtctttatc
    32101 cgcgaccggt acggcaaaat cgagtttgat tggcccgacc ggcgattccc agcgtacgcc
    32161 gaccccggta ccggttttaa agtcgctgcg gcgaatatcg cttaccgctt cgccactatc
    32221 gacaaacacc gcgccccacc attttccggt cacgttgtac tggtactcca gcgatccggt
    32281 tatcaacttc gaggcccctt tcaggtcacc gttggcgtat ttcggagcga tagatttgta
    32341 tttgtagcca cgaatactgc ggtcgccccc ggcgaaaaaa cgcagatccg gcggtacttt
    32401 gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt gtcacaaaac gatggcgatc
    32461 gtacagtgtg cggatccaga cgttttgtgc ctggaaaacg gagaaatcga catctgagcc
    32521 ccaggcagtg ttggagtagt cgatagagta gcgttgcgag tcgccccagg ttggcatcag
    32581 gccaccacga gaacgcgtgc ggctaatcat caccccagga taaaacagca tcgtggtgtt
    32641 ggtaatttca ccctgggtaa agtggtcgag actccagcgc aggttaatgg cacgctgcca
    32701 gccgctggag agatcccagt agcgagaagc caccagcgta gtggagtcag attcggtatc
    32761 gttcaggtca gtgcgcttaa aaccgccctg caccaaataa tattgttcca gtggattctt
    32821 cagcagcggc attttatagc tgaagtcgag gatctgttcc ggcgcggaaa tactggtact
    32881 ggtggtcaga ctgtgaccgt atgagttcat ccacggtttt ttccacgtcg ctttcacgcg
    32941 cggtcccacg tccgtagagt aaccgacccc ggtttcgatg gtgttttctg ttcgcggcga
    33001 aaccacgccc gtcaatggta atactttcgt ttcgcgcgct ttatcaaatt gtggagccac
    33061 caccaccgag ttaaaccagc cggtagcaga aagtcgacgg ttaagttccg ccagatcttt
    33121 cgattcgtac tcatcgccct ctttaaacgg caccagattt tgcaggtatt catcgcggat
    33181 ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt tcgccactgt tataatcaat
    33241 atcccagaag gctttatgca ggccgagcgc aatgcccagc tgcgctttgg taaattcgct
    33301 atcgaaataa cctttacgca acgcaatact ggttaaggac tttttgaaat tttcataatc
    33361 gccctggttc agtaccgtac caatagccgg gcgagtatcg agcaatttca aatagtcttt
    33421 atcggtccgc gcgccgccgc gcaataccac atcggtgccg ccaattaaca ccggcacacc
    33481 tggcgtgact ttggcgatca atacctgccg ccctttcttt ggcggtggac ggagatcaaa
    33541 ttcaatggtc ggctggtaat aacccagcgc tttcagacct tcgcggatgg catcatcgac
    33601 gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt tcaatcgtag aaagctgcgc
    33661 acgaacgttc tttttcagct gtcccgataa cccctcgacc tgtagacgga cgttcgcggc
    33721 gacggcagat ccgcttaagc agagtaagct tacacagcat aactgtcgga tatagcgcac
    33781 attttctcct gaatatcctt ttttcctgcc cctggaacgc cgttaaacgg cttaacaaaa
    33841 atccagtaat atggattaaa aagcagacta aaccccaaat atttcttatg ttttacttta
    33901 gacctattca cggtggttat tgtgtgcaaa tacgcctctt gttacaacct taaccccaat
    33961 gaccgatttt cgggagagcg acaccatgag tttatttgat aaaaagcatc tggtttcccc
    34021 cgccgatgcc ctgcctggac gtaacacccc gatgcccgta gccacgctgc atgcggtcaa
    34081 cggtcactca atgaccaatg tacctgacgg aatggagatt gccatttttg cgatgggttg
    34141 tttctggggt gtggagcgtc tgttctggca gttacccggc gtttacagca ccgccgcagg
    34201 ctataccgga ggctatacgc caaatccgac ttatcgggaa gtgtgctccg gtgatacggg
    34261 tcatgccgaa gcggtacgca ttgtttacga tccttccgtc atcagctatg agcagttgct
    34321 acaggtattt tgggagaatc acgatcccgc ccagggaatg cgtcagggca atgaccacgg
    34381 cacgcagtat cgttcagcga tttatccgct gaccccagaa caggatgccg cagctcgcgc
    34441 cagtctggaa cgttttcagg cggcgatgct tgccgccgat gacgatcgtc acatcaccac
    34501 ggaaatcgct aacgccacac cgttttatta tgccgaagat gaccaccagc aatatctgca
    34561 taaaaaccca tatggttact gtggaattgg cggaattggc gtctgtctgc cgccggaagc
    34621 atagcgttac gggtacaaat gtagtttgtt gataaagtgc tctttgtcta tgcctgatgc
    34681 ggcttaaacg acttaccagg cctacaaaat cgtccaaatt caatatattg caaggactgc
    34741 gtaggcctga tcggcatagc gcatcaggca gtattgcatt tatcagcagt ctgaatttta
    34801 accctctggc gactttacag taccttacgc tatactagcc actgaaaatg ccggttcact
    34861 ttcttcgaat cggctttcaa tgtgtatttc acacaaatta atcaacttcc ccttccgagg
    34921 atctggcctg aaaggtcgga taagatatgt taaacagtat tttagtcata ctctgcttga
    34981 tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct tgccgcctca cgcaaaatca
    35041 aacttaaact gctggctgat gaaggcaata taaatgccca acgcgttctg aatatgcagg
    35101 aaaatcccgg catgttcttt accgtggtcc aaatcggtct gaacgcagtg gcgattctcg
    35161 gcggtatcgt cggtgatgcg gcattttctc cagcttttca cagcctgttc tcccgctata
    35221 tgtcggcaga gctctctgag caactgagct ttattctctc tttctcgtta gtgactggca
    35281 tgtttatcct gtttgcggat ttaaccccga aacgcatcgg tatgattgcg ccagaagctg
    35341 tcgctttgcg tatcatcaac ccgatgcgct tctgcctgta cgtttgcacc ccgctggtgt
    35401 ggttcttcaa cggactggcg aacataatct tccgtatttt caaactgcca atggtacgta
    35461 aagatgacat cacttctgat gacatctacg cggtagtgga agccggtgcg ctggcgggcg
    35521 tgttacgtaa acaggaacac gagctgattg aaaacgtctt tgagctggaa tcccgtaccg
    35581 ttccgtcgtc aatgacaccg cgtgaaaacg tgatttggtt tgatctccac gaagatgagc
    35641 aaagtctgaa gaataaagtg gcggaacatc cgcactctaa gttcctcgtc tgtaatgaag
    35701 atattgacca catcatcggt tatgtcgatt ctaaagacct gctgaaccgc gtgctggcta
    35761 accaaagcct ggcactgaac agcggcgtac aaattcgcaa cacgctgatt gtgccggata
    35821 cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc aggtgaagac ttcgcggtga
    35881 tcatgaacga gtacgcgctg gtagtgggga tcatcaccct caacgacgtg atgaccacgc
    35941 tgatgggcga tctggtcggc caggggctgg aagagcagat tgtcgcccgt gatgagaact
    36001 catggctgat tgacggcggc accccaattg acgacgtcat gcgcgtgctg gatattgacg
    36061 agttcccgca gtcgggcaac tacgaaacca tcggcggctt tatgatgttt atgctgcgta
    36121 agatcccgaa acgcaccgat tcggtgaaat tcgccggcta caaatttgaa gtggtggata
    36181 tcgataacta ccgcatcgac cagctgctgg tgacccggat cgacagcaag gccaccgccc
    36241 tttcgccaaa actgcctgac gctaaagata aagaagaaag cgtcgcgtaa cccccagaaa
    36301 catcaacggc tcctgaatca ggagccgtct tattactgca tagcactttg gttaagccat
    36361 ctctgtttgc agacgcataa cctgacggtt aacttcggac atcactgaca aatgcagctt
    36421 atccttcact tttgggataa gaatcttacc cttatcaaat tcaaaagcgc caacgtcctt
    36481 gatgtataac cgtccacgga acaggatctt tacgtacttc gccacctgaa gtgggttgta
    36541 ccgttggaaa attttcattg ttatctcctg ctgagtatta cgcctgtgcg gtgccacaat
    36601 cggcccaact attatgaggc gcaaatttta atgcttagtg actatagact atccgggcaa
    36661 tgtttccacc gcgtataact ttttttacct aaaggttaca attattcaga attatctttt
    36721 taccgaagcg cggtcttcag tataagcatt catttttcat atgatttgtg cgcttgaccg
    36781 caaactggca tcacacttgc gggaaattcg ataaatagca catatgatta aaactcagac
    36841 ccaagtggtc ggatcacctg catatcataa gaaggaaaca ccatgaccct acgcaagatt
    36901 ctggcactca cctgcctgct gttgccgatg atggcttccg cacatcagtt cgaaaccggt
    36961 cagcgagtgc cgccgattgg cattaccgat cgaggcgagt tggtgcttga taaagatcag
    37021 tttagctaca aaacctggaa cagcgcacag ttagtgggaa aagtgcgagt actgcaacat
    37081 attgctggtc gcacctctgc aaaagagaaa aacgcgacgc tgattgaagc gattaaatca
    37141 gcgaagttac cgcacgatcg ctaccagacc accaccattg ttaacaccga cgacgcgatt
    37201 ccgggttcag ggatgtttgt gcgcagcagt ctggagagta ataaaaagct ttatccctgg
    37261 tcgcagttta ttgtcgatag caatggcgtc gcgcgcggtg cctggcagct ggatgaagaa
    37321 agttccgctg tagtggtgct ggataaggat ggtcgcgtgc aatgggccaa agacggggcg
    37381 ctcacacagg aagaggtgca gcaagtgatg gacctgctgc ataaattaat taataaatag
    37441 acactctgaa tcccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct
    37501 gccagtcatg aacgtgcgct ccggcagctg cagccacagc atgaccagcg gcggtatccc
    37561 aaatattcgt tggtccgaag cgcgggtaca gctgcgcctg cccttccgcc accaggcaga
    37621 atttcagcga agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt
    37681 tcagctccgc atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga
    37741 cctgaatctg cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt
    37801 acattacgtt cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga
    37861 gcgcaatgtt aacggtgaat tcgccattac gtttaataaa ctctttagtg ccatccagcg
    37921 ggtccaccag ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt
    37981 cagaaaggac cggaatctcc ggtgtcagcg tacgtaaacc gtccatgata acggtgtgag
    38041 cggcaatatc cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt
    38101 tcgtcccgtc gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt
    38161 gatctaacat ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac
    38221 cctatcgtta atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt
    38281 ggcattcttc actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga
    38341 tgataaaagg atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc
    38401 cgccagtgtg aatgcagcga cggtcgatct acgtatcatg gaaaccactg atctgcatag
    38461 caacatgatg gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg
    38521 tacggcaagc ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa
    38581 cggcgatttg attcagggga gtccgctggc cgattacata tcggcgaaag gattaaaagc
    38641 aggtgatgtt catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact
    38701 cggcaatcat gaatttaact acggtctgga ttacctgaaa aatgcgttgg cgggagcgaa
    38761 attcccttat gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc
    38821 gtatttaatt aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat
    38881 tggctatatt ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg
    38941 aaaagtgacg gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga
    39001 gaaaggtgcc gatgtcgttg tcgttctggc gcattccggg ctatctgccg atccgtataa
    39061 agtgatggcg gaaaactcag tttattacct cagtgaaatt cctggcgtta acgtcattat
    39121 gtttggccat gctcacgccg ttttcccagg taaagatttt gctgatatcg aaggggctga
    39181 tatcgtatcg aaggggctga tatcgcgaaa ggcacgctga atggtgttcc ggcggtaatg
    39241 ccgggcatgt ggggcgatca tcttggggtg gtcgacttac aactcagtaa taacagcggt
    39301 aaatggcagg tgacgcaggc gaaagcggaa gcacggccga tttacgacat cgccaataaa
    39361 aaatccctcg cggcggaaga cagcaagctg gtagaaacac tcaaagccga tcacgatgcc
    39421 acacgccagt tcgtcagcaa gccaatcggt aaatccgccg acaatatgta tagctatctg
    39481 gcgctggtgc aggacgatcc gaccgtgcag gtggtgaaca acgcgcaaaa agcgtatgtc
    39541 gagcattaca ttcagggcga tccggatctg gcaaaactgc cggtgctttc agctgctgca
    39601 ccgtttaaag ttggtggtcg caaaaatgat ccggcaagct atgtggaggt ggaaaaaggc
    39661 cagttgacct tccgtaatgc cgccgatctt tatctctacc ccaatacgct gattgtggtg
    39721 aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca gtttaaccag
    39781 attgatcctg acaacacgaa accacagtca ctcatcaact gggatggttt ccgcacctat
    39841 aactttgatg tgattgatgg tgtgaattat cagattgatg ttacccagcc tgcccgttat
    39901 gacggcgagt gccagatggt taatgccaat gcggaaagga ttaagaacct gacctttaac
    39961 ggcaagccga ttgatccgaa cgccatgttc ctcgttgcca ccaataacta tcgcgcttac
    40021 ggcggcaaat ttgccggtac gggcgacagc catatcgctt ttgcttcacc ggatgagaac
    40081 cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac gtgcggggga aattcacccg
    40141 gcggcagata acaactggcg tttagcaccg atagccggcg ataagaaact ggatatccgt
    40201 ttcgaaactt ccccgtcaga taaagccgca gcgtttatta aagagaaagg gcaatatccg
    40261 atgaataaag tcgcgaccga tgatatcggg tttgcgattt atcaggtgga tttgagtaag
    40321 taaaacactt ctttttcggc ctataaatca tcaaccgcat ccggcacttt tgtcggatgc
    40381 gatgctggcg catcttatcc gccctacaag ccatgcaccg tagaccagat aagctcagcg
    40441 catccggcag ttatgccgca cgttcatccc gcaccgccaa cacctcgggc aaattcaact
    40501 caatccagtc cgccagtgca gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc
    40561 tgtattccac atgcggcggc accaccggat acgcgatacg gttaagaaaa ccatcctgtt
    40621 ctaacgcctg taacgactgc gcaagcatct tttcactcac cccaccgatt ttgcgccgca
    40681 ggtcgctaaa gcgatgagta ccttcgcgta gcgccaccag aatcaatacc ccccaacggc
    40741 tggtgacgtg tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga ttaccctctt
    40801 tcagttgttg cgacaggcta acctgactca tttcatactt acctttttgt acgtacttac
    40861 taaaagtaag tttaggtgtt agcgtattta aacacaagac aaaacgatgg agacttccca
    40921 tgatcgctat tactggtgcc actggccaac ttggtcacta tgttattgaa tccttgatga
    40981 aaacggttcc tgccagccaa caaatagtgg ctatcgttcg taatccggca aaagcccagg
    41041 ccctggcagc acaaggcatt accgtgcgtc aggctgacta cggcgatgaa gccgcactga
    41101 catctgcact tcagggagtg gaaaaactac tgctgatctc ttccagcgaa gtgggtcaac
    41161 gtgccccgca gcatcgtaat gttattaatg ccgcaaagac ggctggcgtg aaatttatcg
    41221 cttataccag tctgcttcac gcggataaat ctccgctcgg cctcgccgat gagcacatcg
    41281 agacggagaa aatgttggct gattctggca tcgtttacac cctgctgcgc aacggctggt
    41341 acaccgaaaa ctatctcgcc agcgccccgg cagcactgga gcacggcgta tttatcggtg
    41401 ccgcgggcga tggcaaaatt gcctcggcaa cgcgggcaga ttatgcggca gctgcggcac
    41461 gcgtgattag cgaagccggt cacgaaggca aggtttacga gctggcgggc gatagtgcct
    41521 ggacgttgac acagttagcg gcagagctga ccaaacagag cggcaaacag gttacctatc
    41581 aaaatctgag cgaaaccgat ttcgctgcgg cgctgaaaag cgttggcctg cctgacggac
    41641 tggcggatat gctggcggat tctgacgttg gcgcatcgaa aggcggtctg tttgatgaca
    41701 gcaaaacgct gagcaaattg attggccgcc caacgacaac gttagccgaa agcgtaagcc
    41761 atctttttaa tgttaataaa tagttaatta aagtggcatc ctcccgcatc ctctctgata
    41821 atgacgggat gccgggagca atcatgtctg cttcctgaac gtttcttctg acagaccaat
    41881 ggatgccagt aatgattagc ggcgtgctgt acgccctgtt agcagggttg atgtgggggc
    41941 ttatttttgt cgggccgttg atcgtgccgg aatacccggc gatgttgcag tcgatggggc
    42001 gttatctggc gttagggtta attgcgctac ccattgcctg gctgggacgc gtgcgtctgc
    42061 gtcagttggc gcgtcgcgac tggcttaccg ccttgatgct cactatgatg ggcaacctca
    42121 tctattactt ctgccttgcc agtgccattc aacgtactgg cgcgcctgtt tctacgatga
    42181 ttattggcac cctgccggtg gtcattcctg tctttgccaa tctgctttat agccagcgcg
    42241 acggcaaact cgcgtgggga aaactcgccc cggcactgat ttgtattggc atcggcctgg
    42301 cgtgtgtgaa tattgctgag ttaaaccacg gactccccga ttttgactgg gcacgttata
    42361 cctctggcat cgtgctagcg ttagtttccg tggtctgctg ggcatggtat gccctgcgca
    42421 acgcccgctg gctgcgggaa aatcccgaca aacatccgat gatgtgggcg acggcgcagg
    42481 cgctggtcac gctgccggtt tctctcatcg gctatctcgt cgcctgttac tggctgaata
    42541 tacaaacgcc ggacttctcc ttaccttttg gcccccgtcc gctggtgttt attagtctga
    42601 tggttgcgat agccgtgctt tgctcatggg ttggcgcact ctgctggaac gtcgccagcc
    42661 agcgattacc gacagtgatt ctcgggccgc tgatcgtttt cgaaacactg gcaggtttgc
    42721 tgtacacctt tttgatacgc cagcaaatgc cgccgctgat gacgctgagc ggtatcgcgc
    42781 tgttagtggt tggcgtggtc attgcagtca gagcaaaacc ggaaaagcct ttaactgaat
    42841 ctgtctcaga aagttgacat actggcagtg agttaaataa gcctctgcta cgtaagggtt
    42901 atagcatttg ccttaaagat gcatttaaaa tacatcttat cttattaaga atgaggtatc
    42961 agctatggct tatcgcgacc aacctttagg tgaactggcg ctctctatcc ctcgcgcttc
    43021 ggctctgttt cgtaaatatg atatggatta ctgctgcggc ggtaagcaaa cgctggcgcg
    43081 tgcggcggca cgtaaagaac tggatgttga ggtcattgaa gctgagctgg caaagcttgc
    43141 tgaacaaccg attgagaaag actggcgtag cgccccgctg gcagaaatca tcgaccatat
    43201 catcgtgcgc taccacgatc gtcaccgcga gcaactgccg gagctgatcc tgcaagcgac
    43261 taaagtcgag cgcgttcacg ccgacaaacc gagcgtgcca aaagggctga caaaatacct
    43321 gaccatgctg catgaagagc tttccagcca catgatgaaa gaagagcaga tcctcttccc
    43381 gatgatcaaa caaggcatgg gcagccaggc aatggggcca atcagcgtaa tggaaagcga
    43441 gcacgatgaa gcgggcgaac tgctggaagt gatcaaacac actaccaata acgtcacacc
    43501 gccgccagaa gcctgcacca cctggaaagc gatgtataac ggcattaatg aactgattga
    43561 tgacctgatg gatcacatca gtctggaaaa caatgtactg ttcccacgcg cgctggcggg
    43621 tgagtaatct aaaagctgga tggcattgcg ccatccagca tgataatgcg gttatttccg
    43681 cagttcagca gcccgcttct taccaataaa caaccagccc agccccagcg cgataaacca
    43741 cagtggggta accagcagag cctggcgggt gtcatcttcc agcgtcagca gcacgatgac
    43801 gaacacaaag aacgccatac atacccagca catcagcttg ccgagcggca tcttgtagat
    43861 tgacttctca tgcaggtgcg gacgctgttt gcgatacaca aggtatgagc aaaggataat
    43921 cgtccagaca aacataaaca gaatcgcgga aaccgtcgtg atcatcgtga acgcgccaat
    43981 cacgcttgga ttcacgtaca acatcaccac gccgccgagc agacagatac aagagaaggt
    44041 cagccctttc gcgggtactg cgcgtttaga gagtttagcg aacgctttcg gtgcaacacc
    44101 ttcctgcgcc agaccaaaca gcatgcgact ggtagagaag acgccactgt tagcggaaga
    44161 agccgcagac gtcagcacca caaagttgat cacgctggca gcagcaggca gccccaccaa
    44221 cacgaacagt tcaacaaacg ggcttttatc cgggactacc gaactccacg gcgtcacgga
    44281 cataatcaca atcagcgcga agacgtagaa cataatgatg cggatcggaa tagagttaat
    44341 tgcacgcggc agtgatttct ctggatcttt ggtttccgca gcagtagtac ctaccagctc
    44401 aatccccacg aaagcgaaaa ccgctatctg gaatccggca aagaagccac ttaagccttt
    44461 cgggaaccag ccgccgtcat tccacaaatg cgcaaatgac gcttccacac cagtcggtga
    44521 ctgaaagtgc atcgccacca tgaccaggcc gacgacaatc aacgacacga tggcgacgat
    44581 tttgatcatc gcaaaccaga actccatctc accgaacatt ttcacggtgg cgaggttcag
    44641 ggtcagcaac agcactatca ccgccaacga ggcgacccag tcagatagcc ccggaaacca
    44701 gaactgcgca taagccgtga tggcaaccac gtccgccata ccggtaacca cccagcagaa
    44761 ccaataagtc cagccagtga aatatcctgc ccacggccct aataaatcgg aagcgaagtc
    44821 actaaaagat ttgtattcca gattcgaaag cagcaattcc cccattgccc gcatcacgaa
    44881 aaagagcata aaaccgatga tcatataaac gaaaatgatc gacggcccgg caaggctgat
    44941 tgttttaccg gatcccataa acaaccccgt accaatggcg ccgccaatgg caataagctg
    45001 aatatgccgg tttgtgagat tgcgccgtag cgactgttca gccggagcct gatcatcggc
    45061 aacgactttt acctgatcta ccatgttttt tcttcctgtc tgtgttgttc aggctctgcg
    45121 gcctttagtc agtctatgat acgacgaatc tgtatcggga ctcatcgata ttaggaaaca
    45181 atacgtggga tgaatactaa gatttagatc taatgttaat tttatgttta aagtgagtgt
    45241 catatcacgg tgataacgcg aaacagctca caaaaataca cgcttacgga gcgtttgcaa
    45301 cacaaaatat cgccgattgg caaactgaag ggtttattgc taaatgcctg ctcccctctg
    45361 gctcgagggg aacagaatac gctgcttaga ggatttccag cagctcgact tcaaacacca
    45421 gggtgctgaa cggagggatg gatgcgccag cgccgcgctc gccataggcc agttcctgcg
    45481 ggatagtcag ttcccattta gaacctaccg gcatcagagt cagtgcttca atccagccag
    45541 ggatcacgcc attaaccggg aattcagcgg gttcaccacg agcaacggag ctgtcaaaca
    45601 cggtgccgtc gatcagttta ccggtgtaat gaacacgaac gcggtcagta cgtgccggaa
    45661 ttgcgccttc accctggttg atcacgcgga attgcaggcc agattcggtg ctgttcacac
    45721 cttctttttt ggcattttct tcaagatatt tcacaccttc ggcagccatc gcctggaaac
    45781 gctgacgacg aacggcatcg gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat
    45841 caaccggaac agccggatgt ttgccttcca gcgcatctgc aataccagcc accagcgcct
    45901 ccggcagcag cccttgcagg ccagattcac tcagttgttg gcctacctgt aaaccaatgc
    45961 cgtagcttgc ttgcgcttcg atggtgtcaa aagttggggt ggtcatcgta tttcctttct
    46021 tagggtataa aagtagcggg cagcatatca gccacacttc tttgggtaaa actttgtcgc
    46081 aggaatgatg acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat
    46141 aaaggcgggt ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa
    46201 caagacgcgg agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag
    46261 aaagtctggc acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt
    46321 cgggggatca ttattgccgc cattatattg gtggtcggat ttctgcttcc atctgatgat
    46381 acgcccaacg cgccggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa
    46441 ccgccgacag aagaacagct gcgggcgcaa ttagtcacac cacaaaatga tcctgatcag
    46501 gtggctccgg tcgctccaga acctatacag gaaggccagc cagaagaaca gccgcaaact
    46561 acccaaacac aaccattcca gccagatagc ggaattgata atcaatggcg ctcttaccgt
    46621 gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc acggactgcc cgctaccgat
    46681 gtctatgcaa tggcgcaggt agaaggcgcg ggtaagccgc tgagtaattt gcaaaacggg
    46741 caaatggtga aaatccgcca gaacgccagc ggcgtggtga cgggcttaac cattgatacg
    46801 gggaataatc aacaggtcct gttcacccgc cagccggatg gcagttttat tcgtgcgcgg
    46861 tagatgaagg gtcaaccaaa agccagctaa ggattgtctc tttgatgacc ttacgtctgg
    46921 cttccagtgc ctggggggaa accatactga tatgataaac caggccaagc gtatgctggt
    46981 tgatcaaata atatccgcca agcgcagcaa tattaatatt cacctgcaat gggtcgatat
    47041 ctggcttaaa aatattgtgt ttcttccctt catccaataa ggattccatt aactgcaagt
    47101 gcgcatggtt aatttctaag agacgctgtg attttgcata atgcacacct ttactttgat
    47161 tttcactatg cacaattttt aaaaaccagg gattagtaat ataataatcc catgtaaaat
    47221 caataatttt ctcaatggct ttttgtggcg gaagatctga gaaatcaagg gccttttctt
    47281 tttgtcgaat atcgttccag gtatattcca gtacttcaat aaataaattt tctttattac
    47341 caaagtgatg ataaaccgtc tgtttactac accccgcagc cttaacaata ttatcgaccc
    47401 tggccccttc ataaccatat tcggcaaatt cattaaccgc gcataacagc agcttttcct
    47461 tcaggctctg tacgttgctg gtatcaagat acataacgct tccccacgac ctcttcaaga
    47521 taactatctt aacccgatta attagaagct ccagtaaaaa ggataaaaac ttgtactttc
    47581 atcaagttat caaatgtcct gtgatcacta tcgcatatca accaaccaga tggttgatct
    47641 ttcttgcggc acgtcgcaag ctaattgtca cttagaaaac aatattcaaa aaaagagagc
    47701 tattgtatga aatccactcg tcctgttgcc gtgattaccg gagccgctcg tggtattgga
    47761 aagggctgtg cgctggagct tgctcgtggc ggatttaacc tgctgattaa cgatcttccc
    47821 gatgccgaca gcgtagaaaa actgcatatc acacaacaag aatgtatagc tgaaggcgtg
    47881 gaagtgattt gcttccctgc cgatgtcggc gatctctcct tgcatgaaga gatgctcgac
    47941 gcggcgcaaa acctgtgggg acgtctggac tgcctgctca acaacgcagg catttcagtg
    48001 aaaaaacgcg gtgacctcct cgacctcgaa ccagacagct tcgatcaaaa cattgccatt
    48061 aacacccgcg cgccgttctt ccttgctcag gcattcagta agcgcttact ggcacagccg
    48121 aaaccggaag cggagctgcc gcaccgctcg attattttcg tcagttccat caacgccatc
    48181 atgctggcga tgaatcgcgg cgaatacacc attgccaaaa ccgccgtttc tgccgctgct
    48241 cgtctgtttg ctgctcgcct ttgtaatgaa caaattggcg tttatgaagt gcgccctggt
    48301 ctcatcaaaa ccgatatgac tattccagca acggcttatt acgacgaact gattgccaaa
    48361 ggtctggtgc cgtggggacg ttggggttat ccggctgata ttgcttccac cgtccgcgcg
    48421 atggcggaag gtaaactgat ttacacctgt ggtcaagcgg tcgccatcga cggcggctta
    48481 agtatgccgc gcttctgagg ctgaatcatg agagatctgt tacaacgccc ggatttattc
    48541 agtatcaata ccgcgacact gggctataaa acgccgctac ccgccattat tgatgcctgc
    48601 gccgcacgcg gtattggcgc aattgccccc tggcgcaggg agttgcaggg tgaagatctc
    48661 cagcaaatcg cccgccagct tgccgccagt aacatgagcg tttccggttt gtgccgtagc
    48721 acttactaca ccgcgccgac actggcggaa cgcaaactgg cgattgatga taaccgccgg
    48781 gcgctggacg acgcagcggt ccttaacgcc gcctgttata tgcaggtggt tggcgggtta
    48841 ccgcagggaa caaaagattt gtacgaagcg cgggaacaag taaaacaagg gattcgccag
    48901 ttgctgccac attcgaaaga tgtcggcgtg ccaattgcgc tggagccgct gcacccgatg
    48961 actgctgccg accgctcttg cctgtgcacg ttacgtcagg cgctggactg gtgtgatgaa
    49021 ctcgatcccg atggtgagtt tggcctcggc gtggcggtgg atgtttatca cgtctggtgg
    49081 gatccggagc tcgccagtca gatcctgcgt gccggaaaac gcatcctcgc gtttcatgtt
    49141 tccgactggt tagtaccgac caccgatctg gtcaatgacc ggggaatgcc gggagatggc
    49201 gtcatcaata ttccgtcaat tcgtcggctg gttgaaaacg ccggatttaa tggcgctata
    49261 gaactggaaa tattctcgcc ttactggtgg cagaaagata ttaacagcac gctggatatt
    49321 agcgtcgatc gcatcgcgca ttattgttaa ggaatgatca tgagtgacca acccgttctg
    49381 ttcagcagag cggcggcttc ttgccgcctg acattgaacc gtgaagataa atgtcatgct
    49441 attaatgaag aaatgatcga atctctcgac cattatttaa atgaaattga aaacgacacc
    49501 acattacgat tagtcgaatt aacggcaacc ggcgataaat tcttttgtgc gggtggcgat
    49561 attaaatcgt ggtcggccta ttcgccatta gatatgggca gaaaatggat taaacgcggt
    49621 aatgacgttt ttaatcgcct gcgaaactta ccccaattaa ccgttgcgaa tctcaacggt
    49681 cataccatcg gcggtggtat tgaactggcc ttgtgctgcg atattcgtat tgcccgcccc
    49741 ggcgcgaaat tctctaaccc ggaagtgatg ctcggcatgg tgcctggctg gatgggcatt
    49801 gaacgtgtgc ttaatcaggt tggtccggtg gtcggtcgcc agatgctgat gctgggtaaa
    49861 cgcctgacgg cgcaggaagc ccaggcggcg aatttaattg atgaagtggt agaaaaagaa
    49921 caggtggaaa gctggatggc gaaccagtta gcgcagctgg aaaaatgcgg tccggtggca
    49981 ctggcgcata ttaagcaact gattctggca ctggaaaata aacatgccga ttacccgcac
    50041 cagctactgg ccggattaat gtctgccact caggattgcc agcaggcgac gcgcgccttt
    50101 gccgaaaaaa gtagcgtgag cttccataat caataaggaa attgtatgcg taagataaca
    50161 accgccgagg cactggcggc gcaaattcag gacggcgcga ctattgctat tagcggtaac
    50221 ggcggcggta tggtagaagc cgaccatatt ctggctgcta ttgaagcgcg tttcctgcaa
    50281 accggacacc ctcgcgacct gacgctaatc cactcgctgg gtattggcga tcgcgactgc
    50341 aaaggcacta atcgttttgc gcacgcagag atgctcaaac gcattattgc cgggcacttt
    50401 acctggtcgc ccaaaatgca ggcgctggta aaaaataaca cgattgaagc ctactgcttt
    50461 cctggtggcg tgattcaggc actgctacgg gagatcggcg ccggacgtcc ggggcttttt
    50521 acccacgttg ggctgggatc gtttgttgat ccacgcaatg gcggcggtaa gtcgaatgaa
    50581 tgcactaccg acgacctggt agaactgatt gaaatcgacg gtgaaaccaa acttcgttat
    50641 cgccctttca aagtggatta cgcgattttg cgtggcactt atgccgatcc tcgaggcaac
    50701 gtcagccttg aagaagaagc gattgatatg gatagctatt ccatggcgct ggcagcacac
    50761 aacagcggcg gcaaagtgtt cgtacaggta cgcgatgtgc tggaagctgg cgcgattgaa
    50821 ccacgccggg tcaaattacc gggcattctg gttgatggca tcgttgaaca ccgcgaacaa
    50881 ccgcaaacct atcttggcgg ttacgacctg accattagcg gtcaacatcg ccgtctgagt
    50941 tctaacgacg ctattgaact ggttagtcat ccggtgcgcc gcctgattgc ccgtcgggca
    51001 gcacgcgaac tggtggctgg cgcttcaacc aactttggct ttggtattcc tggcggtatt
    51061 ccaggcgtag cgctgcgcga aggcgttcct taccaaagtt tatggctgag cgtcgagcaa
    51121 ggcgtacata acggcatgat gcttgatgat gctctgtttg gttgtgcccg caacgccgat
    51181 gccattattc catcactcga tcaatttgag ttctacagtg gcggagggat cgatatcacc
    51241 ttcctcggca tgggagagat ggatcagtac ggtaacgtca acgtctccca tctcaatggc
    51301 aatctcattg gccccggcgg ttttctcgaa attgcgcaaa acgcccgtaa agtggtgttc
    51361 tgcggcacgt tcgacgccaa aggtagcaag attgatgtaa cgccagatgg cttgcatatc
    51421 gcccagtcag gtcaaatccc taaactggtt acccaggtgg aaaaaatcac ttttagcgcc
    51481 gcctacgcac agcaaagtgg tcaggaagtg ttgtatatca ctgaacgcgc agtattccag
    51541 ttaacggcag aaggcgttga attaattgaa atcgcaccga gtgtggagat tgagcgcgac
    51601 attctgccgt atatggcctt ccgtccaatt atcaagcatc cacgcctgat ggaaagtagc
    51661 ctgtttacgc cgatggagga tgcatgagtc agcttgatga caccatcctt gatgcgctga
    51721 cgcacgttac tttcccaaag ggttttgcac aggcagagcc cgcatgggtt gtcacggtag
    51781 acggtgttga ttacccactc tggcaaacag atgccctggt agtcggcagt ggtgcagccg
    51841 ggctgcgtgc agctgtggaa cttaaacgcc gccagcaaaa tgtgctgatc gccactgccg
    51901 ggttatatat ggggacgtcg gcatgttctg gttccgataa acagacactg tttaccgccg
    51961 ctaccgcggg caacggcgac aacttcacca aactggcaga ggcactggcg agcggtgggg
    52021 cgatggatca cgacaccgct tatgtcgaag cggtaggttc tctgcacact cttggcgggt
    52081 tgcaatatct tggtctggaa ttaccggaag atcgctatgg cgcgattctt cgttatcaaa
    52141 ccgaccatga cgaagccggg cgtgcaacct cgtgtgggcc gcggacctca aggttaatgg
    52201 tgaaagtgct gttggaagaa gtacagcgcc tcgccattcc agtgttgacc agtgcaacag
    52261 tgattaaact gctgcatcag cgtgacgaaa acggcgaaga ccgtgtggcg ggggcaatcc
    52321 tcgcgaccgg tcatcgcgcc cataaccctt gggggctggc aattgtgact gcgcccaatg
    52381 tggtactggc aacaggaggg cctggcgagc tttatcgcga cagtgtgtac ccacacaaat
    52441 gttttggctc gctggggctg gcgctggagg aaggcctgac gctaaccaat ctgaccgaaa
    52501 gccagtttgg tataggcacg ccgcgcagca cgtttccgtg gaatttatcc ggcacctatg
    52561 tacaggtgat cccgtatatc tattccgtgg atgctgaggg taacgagtat aacttcctcg
    52621 cggattacta tcgcaccacc caggagctgg cttcaaacat tttccgtaaa ggctatcagt
    52681 ggccgttcca cgccactcgg gtgatggatt ttggctccag cttgttagat atggcagtag
    52741 cgcaagagca gcaatcaggg cgtcaggtat ttatggattt caatcgcaat cctgaacctg
    52801 tgccgggtga cctgccattc tcattagagc gactggacga cgacgttcgc gcgtatctgg
    52861 aaaataacga cgctctggca ccatcgccca tcgaacgact gcaacgaatg aatccgctgt
    52921 ctatctcgct gtataagatg cacggttacg atctcaccac gcagccattg cagtttgcca
    52981 tgaataatca gcacatgaat ggcggcattg aagtggatat ctggggacaa acatccctgc
    53041 ccggttgttt tgccgtgggg gaagttgctg gcacacacgg cgtcactcgc cctggtggtg
    53101 cggcattgaa tgccgggcag gtttttgctg ttcgtctggc acgttttatt ggttgcacgc
    53161 aaaaacgtaa tattgatgga gatatagcac agctggtagc tcaggcactg gcttctataa
    53221 gagagatcat tactcaggcg cacgataacg ggaccgggat gccgttgtcg gttgtgagag
    53281 aaaaaattca ggcacgaatg tctgaccatg cgggatttat ttgccatgcc gataaagtcc
    53341 gacgcgccac tcgtgatgcc ctgctattga acgaatttgt ccaacggcat ggattggcta
    53401 tcaaacatgt gggcgaagtt gccgagctgt ttatgtggcg gcatatggcg ctgacctctg
    53461 ccgccgtctt aactcaactg acacattata ttgatgctgg tggtggcagt cgtggggcac
    53521 ggatagttat tgatccacaa ggcaaatgcc taccacaaac tcgtcgcggc gcaaaagaag
    53581 aatggcgttt tcgctctgaa cgtgctgaag acaaaaatca cagattaacg attcaatatt
    53641 cgcaaggttc ttttattacc gaagtgaagt ctttacgtat gcaaccgtgt attaacggta
    53701 tttactttga aaaaaactgg ccagactt
//
LOCUS       DAZFZC010000021        51606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFZC010000021 DAZFZC010000000
VERSION     DAZFZC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.021"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..913
                     /locus_tag="ACHKXP_001484"
     CDS             <1..913
                     /locus_tag="ACHKXP_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HFO7646521.1"
                     /translation="GLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVD
                     LVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYS
                     WIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLGRILC
                     RAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAPRGAL
                     GHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEILRTL
                     HSFDPCLACSTHVLGDDGSELISVQVR"
     gene            932..1639
                     /gene="hyaC"
                     /locus_tag="ACHKXP_001485"
     CDS             932..1639
                     /gene="hyaC"
                     /locus_tag="ACHKXP_001485"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO7646522.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            1636..2223
                     /gene="hyaD"
                     /locus_tag="ACHKXP_001486"
     CDS             1636..2223
                     /gene="hyaD"
                     /locus_tag="ACHKXP_001486"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO7646523.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            2220..2618
                     /gene="hyaE"
                     /locus_tag="ACHKXP_001487"
     CDS             2220..2618
                     /gene="hyaE"
                     /locus_tag="ACHKXP_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO7646524.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            2615..3472
                     /gene="hyaF"
                     /locus_tag="ACHKXP_001488"
     CDS             2615..3472
                     /gene="hyaF"
                     /locus_tag="ACHKXP_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO7646525.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            3606..5150
                     /gene="appC"
                     /locus_tag="ACHKXP_001489"
     CDS             3606..5150
                     /gene="appC"
                     /locus_tag="ACHKXP_001489"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO7646526.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRGLEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            5162..6298
                     /gene="appB"
                     /locus_tag="ACHKXP_001490"
     CDS             5162..6298
                     /gene="appB"
                     /locus_tag="ACHKXP_001490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7646527.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            6311..6403
                     /gene="cbdX"
                     /locus_tag="ACHKXP_001491"
     CDS             6311..6403
                     /gene="cbdX"
                     /locus_tag="ACHKXP_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO7646528.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            6483..7781
                     /gene="appA"
                     /locus_tag="ACHKXP_001492"
     CDS             6483..7781
                     /gene="appA"
                     /locus_tag="ACHKXP_001492"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO7646529.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(7896..10076)
                     /gene="etk"
                     /locus_tag="ACHKXP_001493"
     CDS             complement(7896..10076)
                     /gene="etk"
                     /locus_tag="ACHKXP_001493"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO7646530.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDGGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(10096..10542)
                     /gene="etp"
                     /locus_tag="ACHKXP_001494"
                     /gene_synonym="yccY"
     CDS             complement(10096..10542)
                     /gene="etp"
                     /locus_tag="ACHKXP_001494"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO7646531.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVNSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(10530..11669)
                     /gene="gfcE"
                     /locus_tag="ACHKXP_001495"
     CDS             complement(10530..11669)
                     /gene="gfcE"
                     /locus_tag="ACHKXP_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO7646532.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(11715..13811)
                     /gene="gfcD"
                     /locus_tag="ACHKXP_001496"
     CDS             complement(11715..13811)
                     /gene="gfcD"
                     /locus_tag="ACHKXP_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7646533.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPQGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGRFDSEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKKLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(13811..14557)
                     /gene="gfcC"
                     /locus_tag="ACHKXP_001497"
     CDS             complement(13811..14557)
                     /gene="gfcC"
                     /locus_tag="ACHKXP_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7646534.1"
                     /translation="MNKLQSYFIASVLYVMTHHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDRPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(14554..15198)
                     /gene="gfcB"
                     /locus_tag="ACHKXP_001498"
     CDS             complement(14554..15198)
                     /gene="gfcB"
                     /locus_tag="ACHKXP_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO7646535.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(15305..15610)
                     /locus_tag="ACHKXP_001499"
     CDS             complement(15305..15610)
                     /locus_tag="ACHKXP_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646536.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTH"
     gene            complement(16052..16264)
                     /gene="cspH"
                     /locus_tag="ACHKXP_001500"
     CDS             complement(16052..16264)
                     /gene="cspH"
                     /locus_tag="ACHKXP_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO7646537.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            16550..16762
                     /gene="cspG"
                     /locus_tag="ACHKXP_001501"
     CDS             16550..16762
                     /gene="cspG"
                     /locus_tag="ACHKXP_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO7646538.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            16773..16961
                     /gene="ymcF"
                     /locus_tag="ACHKXP_001502"
     CDS             16773..16961
                     /gene="ymcF"
                     /locus_tag="ACHKXP_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO7646539.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            16936..17166
                     /gene="ymcE"
                     /locus_tag="ACHKXP_001503"
     CDS             16936..17166
                     /gene="ymcE"
                     /locus_tag="ACHKXP_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO7646540.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            17156..17329
                     /gene="gnsA"
                     /locus_tag="ACHKXP_001504"
     CDS             17156..17329
                     /gene="gnsA"
                     /locus_tag="ACHKXP_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO7646541.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(17377..18450)
                     /gene="yccM"
                     /locus_tag="ACHKXP_001505"
     CDS             complement(17377..18450)
                     /gene="yccM"
                     /locus_tag="ACHKXP_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7646542.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFIYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFVVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(18522..21266)
                     /gene="torS"
                     /locus_tag="ACHKXP_001506"
     CDS             complement(18522..21266)
                     /gene="torS"
                     /locus_tag="ACHKXP_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO7646543.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMISLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMHIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            21349..22377
                     /gene="torT"
                     /locus_tag="ACHKXP_001507"
     CDS             21349..22377
                     /gene="torT"
                     /locus_tag="ACHKXP_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO7646544.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(22350..23042)
                     /gene="torR"
                     /locus_tag="ACHKXP_001508"
     CDS             complement(22350..23042)
                     /gene="torR"
                     /locus_tag="ACHKXP_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO7646545.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QSVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            23172..24344
                     /gene="torC"
                     /locus_tag="ACHKXP_001509"
     CDS             23172..24344
                     /gene="torC"
                     /locus_tag="ACHKXP_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO7646546.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            24344..26890
                     /gene="torA"
                     /locus_tag="ACHKXP_001510"
     CDS             24344..26890
                     /gene="torA"
                     /locus_tag="ACHKXP_001510"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO7646547.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAVKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPQLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKHIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            26887..27486
                     /gene="torD"
                     /locus_tag="ACHKXP_001511"
     CDS             26887..27486
                     /gene="torD"
                     /locus_tag="ACHKXP_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO7646548.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(27638..27943)
                     /gene="cbpM"
                     /locus_tag="ACHKXP_001512"
     CDS             complement(27638..27943)
                     /gene="cbpM"
                     /locus_tag="ACHKXP_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO7646549.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(27943..28863)
                     /gene="cbpA"
                     /locus_tag="ACHKXP_001513"
     CDS             complement(27943..28863)
                     /gene="cbpA"
                     /locus_tag="ACHKXP_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO7646550.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            29124..30379
                     /gene="yccE"
                     /locus_tag="ACHKXP_001514"
                     /pseudo
     CDS             29124..30379
                     /gene="yccE"
                     /locus_tag="ACHKXP_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            30672..31913
                     /gene="agp"
                     /locus_tag="ACHKXP_001515"
     CDS             30672..31913
                     /gene="agp"
                     /locus_tag="ACHKXP_001515"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO7646551.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(31951..32178)
                     /gene="yccJ"
                     /locus_tag="ACHKXP_001516"
     CDS             complement(31951..32178)
                     /gene="yccJ"
                     /locus_tag="ACHKXP_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO7646552.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(32199..32795)
                     /gene="wrbA"
                     /locus_tag="ACHKXP_001517"
     CDS             complement(32199..32795)
                     /gene="wrbA"
                     /locus_tag="ACHKXP_001517"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7646553.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            33168..33341
                     /gene="ymdF"
                     /locus_tag="ACHKXP_001518"
     CDS             33168..33341
                     /gene="ymdF"
                     /locus_tag="ACHKXP_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7646554.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(33440..33538)
                     /locus_tag="ACHKXP_001519"
                     /pseudo
     CDS             complement(33440..33538)
                     /locus_tag="ACHKXP_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33599..34927)
                     /gene="rutG"
                     /locus_tag="ACHKXP_001520"
     CDS             complement(33599..34927)
                     /gene="rutG"
                     /locus_tag="ACHKXP_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO7646555.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGMNPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDYTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(34948..35442)
                     /gene="rutF"
                     /locus_tag="ACHKXP_001521"
     CDS             complement(34948..35442)
                     /gene="rutF"
                     /locus_tag="ACHKXP_001521"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO7646556.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(35453..36043)
                     /gene="rutE"
                     /locus_tag="ACHKXP_001522"
     CDS             complement(35453..36043)
                     /gene="rutE"
                     /locus_tag="ACHKXP_001522"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO7646557.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFNRQYVDDAFFA
                     GSTLKSNLLINIGYGDNSKLYARLPRLSFEEACGLL"
     gene            complement(36053..36853)
                     /gene="rutD"
                     /locus_tag="ACHKXP_001523"
     CDS             complement(36053..36853)
                     /gene="rutD"
                     /locus_tag="ACHKXP_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO7646558.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPMQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(36861..37247)
                     /gene="rutC"
                     /locus_tag="ACHKXP_001524"
     CDS             complement(36861..37247)
                     /gene="rutC"
                     /locus_tag="ACHKXP_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO7646559.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNMLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(37259..37951)
                     /gene="rutB"
                     /locus_tag="ACHKXP_001525"
     CDS             complement(37259..37951)
                     /gene="rutB"
                     /locus_tag="ACHKXP_001525"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO7646560.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(37951..39042)
                     /gene="rutA"
                     /locus_tag="ACHKXP_001526"
     CDS             complement(37951..39042)
                     /gene="rutA"
                     /locus_tag="ACHKXP_001526"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO7646561.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            39330..39968
                     /gene="rutR"
                     /locus_tag="ACHKXP_001527"
     CDS             39330..39968
                     /gene="rutR"
                     /locus_tag="ACHKXP_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO7646562.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            complement(40008..43970)
                     /gene="putA"
                     /locus_tag="ACHKXP_001528"
     CDS             complement(40008..43970)
                     /gene="putA"
                     /locus_tag="ACHKXP_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7646563.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            44025..44234
                     /locus_tag="ACHKXP_001529"
     CDS             44025..44234
                     /locus_tag="ACHKXP_001529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646564.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            44393..45901
                     /gene="putP"
                     /locus_tag="ACHKXP_001530"
     CDS             44393..45901
                     /gene="putP"
                     /locus_tag="ACHKXP_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO7646565.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            46566..47396
                     /gene="efeU"
                     /locus_tag="ACHKXP_001531"
     CDS             46566..47396
                     /gene="efeU"
                     /locus_tag="ACHKXP_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO7646566.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            47454..48581
                     /gene="efeO"
                     /locus_tag="ACHKXP_001532"
     CDS             47454..48581
                     /gene="efeO"
                     /locus_tag="ACHKXP_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO7646567.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            48587..49858
                     /gene="efeB"
                     /locus_tag="ACHKXP_001533"
     CDS             48587..49858
                     /gene="efeB"
                     /locus_tag="ACHKXP_001533"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO7646568.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYFGSALLRV"
     gene            50479..51267
                     /gene="phoH"
                     /locus_tag="ACHKXP_001534"
     CDS             50479..51267
                     /gene="phoH"
                     /locus_tag="ACHKXP_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO7646569.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT        12273 a        13128 c        13668 g        12537 t
ORIGIN      
        1 tggtctttct gataaatgcg ttctcagcta cggcgcattc ccggatattg ccaacgactt
       61 tggcgagaaa agtctgctga tgcctggcgg cgcggtgatt aacggcgact tcaacaatgt
      121 gctgccagtg gatttggttg atccgcagca ggtgcaggag tttgtcgacc acgcctggta
      181 tcgatatccc aacgatcagg tcgggcgtca tccgttcgat ggcatcaccg acccgtggta
      241 caaccccggc gatgtcaaag gcagcgatac caacattcag cagctgaatg aacaggaacg
      301 ctactcgtgg atcaaagcgc cacgctggcg cggtaacgcg atggaagtgg ggccgctggc
      361 gcgcacgtta atcgcttatc acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat
      421 gatgtcggcg ttgaacctgc cgctttccgg tatccagtca acgttaggcc gcattttgtg
      481 ccgcgcgcac gaagcgcagt gggccgcagg taagttgcag tatttcttcg acaagctgat
      541 gaccaacctg aaaaacggca atctcgccac tgcttccacg gaaaaatggg aacctgcaac
      601 ctggccgaca gagtgccgtg gtgtcggttt taccgaagcg ccgcgcgggg cgttaggcca
      661 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
      721 gaacgccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa
      781 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
      841 cccgtgcctc gcctgttcaa cacacgtgct gggcgacgac ggtagcgagc tgatctccgt
      901 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
      961 ccactatgtc tttgaagcgc cagtgcgcat ctggcactgg ttgacggtgt tatgcatggc
     1021 ggtgttgatg gtcaccggat actttatcgg caagccgcta ccttccgtca gcggcgaggc
     1081 gacgtatctg ttctatatgg gctacatcag gttaattcac ttcagcgccg ggatggtttt
     1141 taccgtggtt ttgctgatgc ggatctactg ggcttttgtt ggcaatcgat actcccgcga
     1201 gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
     1261 ctggtatctg tttctggcaa aacgtccgag tgccgatata ggccataatc ccatcgccca
     1321 ggcggcgatg ttcggctatt tcctgatgtc ggtctttatg atcatcactg gttttgcgct
     1381 gtacagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
     1441 ctactggacg ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
     1501 gattggcgcg tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga
     1561 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
     1621 taacaaggag cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
     1681 tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg
     1741 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
     1801 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaacg
     1861 ctgcgaacct atgccggaga acgcattccg gcttatctca gcgcgaagaa aatgagcctg
     1921 catcagaaca gtttctccga agtgttggcg ctggcggata tccgcggaca tctgccagca
     1981 catattgccc tcgtcggtct gcaacccgca atgctcgacg actacggcgg tagcctgagc
     2041 gaactggcac gggagcaact gcccgctgcg gaacaggcgg cgctggcgca gcttgctgcg
     2101 tggggaattg tgccgcaacc ggctaatgaa tcgcgctgtc tcaattatga ctgtctgtcg
     2161 atggaaaatt acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga
     2221 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
     2281 cagtcagtga atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat
     2341 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
     2401 tactgcgcga gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg
     2461 aagccatcgg cgatcgcttt ggcgtctttc gctttcctgc cactttagtg tttaccggcg
     2521 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
     2581 gcgggcttgt cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct
     2641 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg
     2701 tcaggtgaat gatgaaccga gtatggcggc cctggagcaa tgtgctcaca gcccgcaggt
     2761 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccaaccgc cgttgggcga
     2821 ggtgctggca gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct
     2881 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct gacgacagtg aaagtgaaat
     2941 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
     3001 ggaggacaaa ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
     3061 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
     3121 gttggcgcat gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa
     3181 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
     3241 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaacg ccacggggtt
     3301 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
     3361 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
     3421 gcagcggctt agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg
     3481 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
     3541 tcaggataaa gagggagatc taccattatc gggttatttt tctctcttcg cctacaggag
     3601 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
     3661 actttttatt tgtacccctt accctggggc tgattttttt gctggctatt atggaaacca
     3721 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
     3781 tcggtatcaa ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
     3841 ctaactggtc attctattcc aactatgtgg gcgatatttt tggcgcaccg ctggcgatgg
     3901 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc
     3961 aacggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
     4021 tctctgcgtt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
     4081 ttgatatcga caccctgcgt atggagatga ccagcttcag cgagctggtc tttaatccgg
     4141 tcagccaggt gaaatttgtg cacaccgtaa tggcgggcta cgtgaccggg gccatgttta
     4201 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct
     4261 cgtttgccat cggttccgtc ttcggtactc tggcgattat cggtactctg caactcggag
     4321 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
     4381 agtggcaaac ggaacctgca cctgcaccgt tccatgtggt tgcctggccg gaacaggatc
     4441 aagagcgtaa cgcctttgcc ctcaaaattc ccgcgctgct agggatcctc gccactcact
     4501 cattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
     4561 aacgcggacg tatggcctgg ctgttaatgc aggaaatatc gcaaggcaat cgtgagccgc
     4621 atgtgttgca ggcattccgg ggactggaag gtgacctggg ctacggcatg ttgctctccc
     4681 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
     4741 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
     4801 tgctgctact ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
     4861 atcgctgggt gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag
     4921 ccgggtggtt tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga
     4981 catactccgc gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
     5041 tagggcttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
     5101 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
     5161 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
     5221 ggtctttatg atctccgacg gatttgacat ggggatcggc tgtctgctgc cgctggtggc
     5281 gcgtaatgat gatgaacgcc ggatagtgat aaacagcgtt ggtgcacact gggaaggcaa
     5341 ccaggtctgg ttgatcctcg ctggtggggc attatttgcc gcctggccca gagtgtatgc
     5401 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcac tgttcttccg
     5461 cccgctggcc tttgattatc gcggaaaaat cgccgatgca cgctggcgta aaatgtggga
     5521 cgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
     5581 cttgttgctc ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag
     5641 cttctggcaa ctgctgacgc cattcccttt attgtgcgga ttgctcagcc ttgggatggt
     5701 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
     5761 acagctggcg accaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcgggtta
     5821 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
     5881 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata attttgtcga
     5941 gtcgcccgtt ttgtggatct tcccgctgct gggattcttc tgcccattgc tgacggtgat
     6001 ggcgatttat cgtggtcgcc cgggttgggg atttttgatg gcatcattga tgcaatttgg
     6061 cgtgattttc acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gcgtgagtcc
     6121 gatctccagc ctgacgttgt gggacagtac ttccagtcag ctgacgctga gcattatgtt
     6181 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
     6241 aatgtggggg cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg
     6301 agcagaaaca atgtggtatt tactttggtt cgtcggcatt ttgttgatgt gttcgctctc
     6361 cacccttgtg ttggtatggc tggacccgcg tctgaaaagt taacgaacgt aggcctgatg
     6421 cggcgcatta gcatcgcatc aggcaatcaa taatgtcaga tatgaaaagc ggaaacatat
     6481 cgatgaaagc gatcttaatc ccatttttat ctcttctgat tccgttaacc ccgcaatctg
     6541 cattcgctca gagtgagccg gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg
     6601 gtgtgcgtgc tccaaccaag gccacgcaac tgatgcagga tgtcacccca gacgcatggc
     6661 caacctggcc ggtaaaactg ggttggctga caccgcgcgg tggtgagcta atcgcctatc
     6721 tcggacatta ccaacgccag cgtctggtag ccgacggatt gctggcgaaa aagggctgcc
     6781 cgcagtctgg tcaggtcgcg attattgctg atgtcgacga gcgtacccgt aaaacaggcg
     6841 aagccttcgc cgccgggctg gcacctgact gtgcaataac cgtacatacc caggcagata
     6901 cgtccagtcc cgatccgtta tttaatcctc taaaaactgg cgtttgccaa ctggataacg
     6961 cgaacgtgac tgacgcgatc ctcagcaggg caggagggtc aattgctgac tttaccgggc
     7021 atcggcaaac ggcgtttcgc gaactggaac gggtgcttaa ttttccgcaa tcaaacttgt
     7081 gccttaaacg tgagaaacag gacgaaagct gttcattaac gcaggcatta ccatcggaac
     7141 tcaaggtgag cgccgacaat gtctcattaa ccggtgcggt aagcctcgca tcaatgctga
     7201 cggagatatt tctcctgcaa caagcacagg gaatgccgga gccggggtgg ggaaggatca
     7261 ccgattcaca ccagtggaac accttgctaa gtttgcataa cgcgcaattt tatttgctac
     7321 aacgcacgcc agaggttgcc cgcagccgcg ccaccccgtt attagatttg atcaagacag
     7381 cgttgacgcc ccatccaccg caaaaacagg cgtatggtgt gacattaccc acttcagtgc
     7441 tgtttatcgc cggacacgat actaatctgg caaatctcgg cggcgcactg gagctcaact
     7501 ggacgcttcc cggtcagccg gataacacgc cgccaggtgg tgaactggtg tttgaacgct
     7561 ggcgtcggct aagcgataac agccagtgga ttcaggtttc gctggtcttc cagactttac
     7621 agcagatgcg tgataaaacg ccgctgtcat taaatacgcc gcccggagag gtgaaactga
     7681 ccctggcagg atgtgaagag cgaaatgcgc agggcatgtg ttcgttggca ggttttacgc
     7741 aaatcgtgaa tgaagcacgc ataccggcgt gcagtttgta atgcataaaa aagagcattc
     7801 agttacctga atgctctgag gctgatgaca aacgaagaac tgtctaatgc gtagaccgga
     7861 aaaggcgttc acgccgcatc cggccacttt cagttttact ctttctcgga gtaactataa
     7921 ccgtaatagt tatagccgta actgtaagcg gtgctggcgc gtttaatcac accattgagg
     7981 atagcgcctt taatattgac gcctgcctgt tccagacgct gcattgacaa actcacctct
     8041 ttggcggtgt tcaagccaaa acgcgcaacc agcaggctgg tgccaacaga acgccccacg
     8101 accgcggcat cactcaccgc cagcatcggc ggcgtatcga caatcaccag atcgtaatgg
     8161 tcgttcgccc attccagtaa ttgacgcatc cgatcgcgca tcagcagttc agacgggtta
     8221 ggtggcacct gaccgcgagt aatcacatca aagcctcctt tgccaaaatg ctggatcact
     8281 ttgttgagct catctttacc tgccagatat tccgacaagc catgttcatt actcacggta
     8341 aacaggttat gcgaataacc acggcgtaag tcggcatcaa taaataacac tttttgatcg
     8401 gactgggcga tcaccgctgc cagagttgaa ctgacaaacg ttttaccacc gtctggcgtc
     8461 gcaccggtga tcatcagaat gttattctcc gtctccatca tagcgaaatg cagactggtt
     8521 cgtagcgcac gtacggcttc cacagcagaa tccgccgggt tatccaccgc caggaagggg
     8581 atatttttag tacgatggcg ctgctgatta gaaaataaat ttttcttacg cagacgggtg
     8641 cgtttatcca gccactcgga cattgggata gtggcataaa cgctgatgcc gtgctcttcc
     8701 agttgttccg gtgcttctac accacgacgc aacatcgcac gcgccagcac ggcacccacc
     8761 gaaataaaca ggccaagaat aaaaccaagc accacgttca acgctttttt cggtttcact
     8821 ggctgcggct gagtgactgc cgggtcgata atccgcacgt taccaatggc actggatttc
     8881 gaaatactca actcctgctg gcggttaagt aattgcagat ataccgcacg gcccgcttct
     8941 acgtcacgac ttaaacgcaa cacttcctgt tgggtggaag gcatagccga tacccgctta
     9001 ttcaggcgtt tgcgttcttg ctccagcgtc tggcgttttt ccagcagcgc acgataagtt
     9061 gggtgatctt tcttatacag ctgggagatc tctgcctcgc ggaaggtcag ctcattgagt
     9121 tgattatcaa cgttaacaat ctgctcaaga acggctttgg cttccaggtt aaggtcaacc
     9181 gaatcgcgct gctggcgata aacgttgagt ttttcttccg cctggtccag ctcgctgcgc
     9241 acttcaggca gctggcgctg taagaattca aggctttgtg aatcctgcgc cgcctggcga
     9301 gcgatattct gttgcaaata gttgttagcg atgctgttca gaatacgagt aatcaactgg
     9361 ggatcatcac ccgtcatggt aagttccagc atcccgcttt ctttactgcg ttcgctaacg
     9421 gtaaaggttt cctgcaacgc gttaatcgct tccagttcgg tacgctggct caggacaaac
     9481 tgtgttcctg gtttggcctt aatatccgcg atagtcagcg caacgccatc tttttccaga
     9541 cgctggccga ccataccatt gacggtgaac tcttcacctt ccagcgtata gtggccgttt
     9601 tccccaaccg tgagtgtcag ttgctgatcc tgaccattca gttgtggaat atgcatccag
     9661 ctgatcgcca gctcacctgg tttttctttg gttaatctcg cccagccgcg acccacaatc
     9721 ggaaaatact tctgctcaac catgtcgcgc agattcagtt cagcaatggt tttaccgaga
     9781 atcatgcgcg attgcagcag ttggatctcc ggtgcagatt cgggcgatga gttagggatc
     9841 atatcgctca ggccgctgag aatggcgttg ccctgtttct gctcaacctg gaccagagta
     9901 tctgcctgat aaattggtgt gcttaacagc gagtaagcga cagcgatcag cgtgaataac
     9961 gcggtcacgc tgataataaa cttacggtga tcccataact cgccgaccag acgaagcaga
    10021 tcgatctcat tttcctgagt gctgcctggt ggcgtattca tatttttagt tgtcatcgta
    10081 ttttataaac tcaaattacc ggctgaggcg cttcgcccat tcctgactgg cgcgctccaa
    10141 cataccgtag acatgttcaa atgcgtcctg acttttacga tagggatccg ggatctcttt
    10201 ctgttccagc cattgcccaa acagcattgt cttcccgcgg acctcgggag cgattgcggt
    10261 aacctgagca atatgttccg actccattgc cagaatcaga tcgtaatttc gtgccatctc
    10321 agcagtgagc ttacgtccgg catgtccttc aagagatacg ccatgattag cggcaacatc
    10381 tgccgctgtc gcatctgcag ggtgttttac cagaccatga acacctgccg aattgacttt
    10441 tacgcccggc agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc aaatgttgcc
    10501 ggtacaaacc accaggattg agttaaattt tagttgggcc atgtttgaat ccaccgtgaa
    10561 gtctctgtca ggtcatgcac cccggaaatc gtcggtacca gcagagaaat cacacggttc
    10621 caacgagcca gcggcgcggt agtgacataa acaatgtcgt aaggttcaag ctggaattcc
    10681 gttccgagta tcattgccga tgcgtccttc gcattcagct gataaatatt ggcgattttg
    10741 ccattctgct tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc agcgacatcc
    10801 tgattcatac cctcagcgtt acccagcgct tccgccaggg tcataccgct gcggtccatt
    10861 ttcagcgtgc tctgtttacc cacttcaccc atcacgaaga ctttcagatc atcgttgcgc
    10921 ggaataaata aaatatcgcc cgggtgcagc agtttgttct ggcgcaagtc gccacgctgc
    10981 atcagcgcat agaggttcac tttggtcttt acgccattct gggtgagcac cacgttgcgc
    11041 cagtctgcat ctgcggtcag accgcccgcg gcgttaatcg catccatgat ggtcagagga
    11101 atattggtaa tcggctgctg acccgatttg ctgacttcgc cggtgacgta cgctttttgc
    11161 gagcggaacg ccgcgacgct gacatcgact tgcgggcttt caatgactga atccagacgc
    11221 gctgtaatct cgttgcgtac ctgggtaagt gttttcccgg ccactttcag gcgaccaata
    11281 taggggtaga aaatcgcacc atcggcgtta acccagttgc cggtatcact ggcgctacgg
    11341 tattgccctg cgggggtggt tagttccggg tgatcccaga ccgtaaccat caacacatca
    11401 ccaatgccga tacgatattc atagttcgcg atttgctgct ccagctccgg gttagcctgc
    11461 gacataatcg gtttggcgcg cagctgatca atcaatcctg gcgtgacggg atagatattg
    11521 accattttgt ccaggtcata ctgattgtca ggtagttcaa tgacatcctt gttactggtc
    11581 gataaattct gaccaggaac aagggtacat gctgtcaaag acagaacagc caatgtcagt
    11641 accgaaaact taaaaatgtt ctttttcatt ggtttatatt tatcagtcaa tgtaatgtta
    11701 tgaattacgt caggttattg tccaaccggt acttctcgct ctgccgtcat tggatagagc
    11761 tggtatttgc gtgacagcat ttgtccacca tcacgcgtca atggtgtcca cgaaaccacc
    11821 gcgcggttgc ggttaggtcc aatggtcatc aagtcgaacg gaatcgagat ataaaaacct
    11881 ttactaaagc cgccttcgcc gtagtcatct ttagatacgt tactgattgc cgcccatacc
    11941 cctaccgcca cgccgctgtc aaagcgtttg gcgacatcga tcgttgcgcc tttatctttt
    12001 gccagatatt gcccaacgct aagtttcatc agtacgccat tgagcgtcgg cgggttccag
    12061 taagccgtca cgaaaccagt tggcgtggaa taatcggtga agcgcatcat gttgtcccag
    12121 tcacgctgct tcacgtagtt aacgtccaca cccagcgccc agctggcatc tagcgggcga
    12181 taaagcagct cggaaccgac accggcatac atcgtttcca gataaccgcc atacacctgg
    12241 ccatagaaac cattgcctaa gtcggcaaag tagttcgcct gcaagttgtt gagataaacg
    12301 tcattgcgaa cgtaatcacg gatatgcgtg cgcacgcggg gcagggtaga gtccgcgggt
    12361 aatagcgaag acttaaactt gtcgtagtta ttgtaaatat tggtgaaaat accgccatca
    12421 agcagcaggt ggtcagtaaa ccagtagcgg gcgctggaca tcagccccaa ctggaacata
    12481 tagaaatctt ccgggccgcc cagcgattgt gaaagtgttg ggttgaaaga gtagctaaag
    12541 cgatcttcac gaatacggta gccccgacca aaggcagaaa gatcttctgc ttcaacacgt
    12601 tgttgttgca gttgctctga tttaccaggc gctgtaccag ccagcttttt gcgcaggctg
    12661 gctacgtcgg tttcggtagt caccatcgcc atatgctcgc gcttttgcgt cacgctaatc
    12721 ttctcaacgc cttgcggcag gttattcacc agaatccggt tggcacgatc gaccgcttca
    12781 cgagagtcac ggtatttgta ttgctgacca gacatataca gtgtcttatc gcgtagctga
    12841 atttctggcg cgtcaaagcc cgcgttatac ttcagggcgg taagttggtt tgctaccgtg
    12901 gtgtactgca atccttcaga ttcaggcgca ggttgatatg ccggttttgg cgtatcgcgc
    12961 agggcagggc gcagatcgtt gaaattggtc cgtaacgtga agccaaacat caacgtgtta
    13021 ccgcgttcat aactcaggtt gagatctgcc cagctggcag cgcgataaac tgcgccgacg
    13081 ttgaaatggc ttgcctgagg cagtttgcca gcgaaatcat tctggtaatt gtttccatcg
    13141 tattcgagtt tcagacgcag gggattccac ggcgtttgat attcaatgcc gccaaagatg
    13201 gaggctgggc cacgaaagat atcgctaaag ctgatatcgc ccgcatcgtg agactctgcg
    13261 cgatgacaat atttatcgct cacccggcaa aacgggttgg taatattgcc cgcattaccg
    13321 gcgtagcccc atgccatccc gagggtgaaa tcaaatggcc ccgcttgctt gctggccacc
    13381 agatactcac tgtcaaaccg acccgtacca gcaatatcac gtttaccaaa cgccacttgc
    13441 ggtagccagt aaccttcttc ccacaggcga agtttaaaat cgaatgattt gtctttgtat
    13501 gactgatcgc cgctgaaatc ttcccactgg ctatatttgc gtgtgcgcac atccgtataa
    13561 cgaatggtgc cttccagcca tgggaacagc gccacggagg tggagtagaa acggtattga
    13621 tcgttatccc ggtagttgac gctgaattca ccttccggtg cgatgcgcgc atttggcatc
    13681 tgcaacaatc ctgtgccgcc aaagtccgat tgcgacgaac cctgcggatc cgggtaggtt
    13741 aatacttcag catgacaggc actggagacg gcaatggcca ggcagcttaa aagatatgaa
    13801 ttcttcttca tcagtcagga acacgttgcg ttaaaacaga cacaatctgg tcatttaaat
    13861 cagcatattt ttcgggcagt acatgcgcag aaaagcccaa ccaaagctgg ctgccaggcg
    13921 gtggttccac atgtctttta ttccacaggg caacaggggc gacgacagtc tcaccctcag
    13981 gcgtaatcac cataacgtta ttcttgtccg cgcctgcaag acgggggcga tcttgcagat
    14041 aatcggtaac gctacggcct gcctgccagg gcaattgccc agccccagaa accgcaccca
    14101 gcagagtaat agtcaccgga cgctgtacgg tatagagcgt atagtccccg accagcgggg
    14161 gattgctgtt ctcatccact cgtacaaaat cagggtccag tttgaccggt aagcgaccag
    14221 tgatattgag attgagtaac tgctggcgca cggatttaat cgttgccacc acatcgtcgt
    14281 cagcttctgc ttcccaggaa gcaagctgtg ccatgacatg ttggtagtct ttaagcgctt
    14341 tagctttcgc cgcagagtcg gtcagcaagg caccaggcca ccagaggcga tcgcgcagtt
    14401 gcggttgtgt caccaactgg acaacatttt ccacggggcc aacggaaagt gtctgttgtt
    14461 cgccaggcag ataaatagtc accgttcctt gcgcaaaggc atggtgtgtc attacgtaaa
    14521 gtacgctggc aatgaaatac gactgtaatt tattcatggt gcgggtttaa gaaacgtcat
    14581 ttctacggga ataacccctg cgcctaacat ttgccgactt tgacgaacct ggccagtagc
    14641 cgaatcgacc cagaatgtgt tttgccactg tttttctggc agcgtggaag tcacctcttc
    14701 ctgccagaca ttacacagca cattgcgacc cgcaatattc agcgtctcta atccagcaaa
    14761 ggaaaaagta gaactcaggg tcgcagagcg aaaatggttg tcttcactcc agagaatatc
    14821 gcgcgtccag cggctgccat cttttattgc cagcgcgtta cgcagagggt cctgcccgga
    14881 atttgtcact tccagcagat tattattaag tttgacggtt tttaaaagac gtccattgtg
    14941 ggtaaccagc atggcgttat cctgggataa ccatttgctt tgttcttgtt ctatatatcc
    15001 cagtaccaca aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg aataaggcaa
    15061 cgcctggatc tgctgatccg ctacggtgat atcctgatta tcgaaaaggc tggcgcgaaa
    15121 tgtatcgacc atactttgct ggctgtgcgt acatcccgca agaaatagtg cgaaaatcga
    15181 taaaataaga gggcgcacct ttaatccttg aaaggttaat taaaaaaatg caaatctcta
    15241 aagcccggta cgaatatcgc aaccgggtat catttaactt taatttcgac gcgttaaagc
    15301 agtattagtg tgtagtggac gtggtggttg aggtgttatg agaaccgcca tcgccactgt
    15361 catttgctgc aacaactgcc aacgtcgcca caccaacagc cgttgccaca ccgacaactg
    15421 tcgccgtgcc gacagcgcca gcagccacgc caccagtggt agtagcagtc gtggttgcgc
    15481 cggtagtggt cgttgtggtg ccagtagtac ccgtcgttgc ctcggtcccg tttgttgctt
    15541 caggggcagc aaacgcagct ggcgtcgtca acatgaaggc cataaggatt gcagaaagct
    15601 tgtgtttcat aacttttcct ttattcatcg catggacaat acgtacgctt gagatcaaga
    15661 ttgtctactt tgagtataac atccttgtga aatctccctt tcctcatacc gcttgcacta
    15721 tatactgcgg gtaggaaagg cgcgcagagg gaaatataag attgttaact atccccctct
    15781 ggaaatacga cttgtgagca taagataaat cttagcaaaa tacaaattaa agataaggaa
    15841 attgttacga aagctattaa ttattgttgg taatatagtt tcaagtggta ctataactgg
    15901 ctgttaaatg gcatttataa ctattaggtg cagagatatt cgcttaaatg gattagtttc
    15961 tttgtggaaa agtaactgat gttataatgt atgatgttga ctattgcact attccaataa
    16021 aaccagtcag ctttaaacaa gcagcgtcat attaagagag ataaacattt gccgctgttg
    16081 gtcctcgcag gccatttacg cggcaaaatt ccacacgtaa tcctggtata agcacttctg
    16141 cgtcgcgggg agtgaatgcg gaaatatgga cctgaacttc tttacgaccg tcagagggga
    16201 taatgaatcc tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg
    16261 acaaacaaat tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc
    16321 cagcgatatt taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg
    16381 cgttaatagc tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt
    16441 tctcattatc aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc
    16501 tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa
    16561 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
    16621 tgatggcagc aaagacgttt tcgtccattt cactgccatc cagagcaatg aattccgcac
    16681 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
    16741 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
    16801 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
    16861 tgggcgctaa gtgtattttt tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc
    16921 aaatacgcac ggaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
    16981 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
    17041 ttgattattg aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg
    17101 ggaaccaaca ccttactggc gtgttgctgt cttttaagac cagaagaggt taacagtgaa
    17161 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
    17221 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga
    17281 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
    17341 tcacccgtgc gaggtgatgt tccgcttgtt gctaatttag tgaccaatca ttggcgcttg
    17401 tggaattaag cgtcggtaca attcatccgg taccgggctt tgccatactc ccgcatacat
    17461 tgcgtaacca atcaccacaa acataatccc cagaaccagt agcgtcatta accagccaga
    17521 caacgcaaag gcttttttat ttgccgcagg tttttgcagt gaaaaggtca atgttgaggc
    17581 taccggacat gactctacgc aagtcataca gccggtacat tccactgttc gtacctgaat
    17641 taatttatcg accgggatcc gtgatgggca attttttgcg catttgccac agtcgataca
    17701 actttcggca ttgcgacgaa tcttaaacgg cgacaatagc gaaaccacgc ccatcagcgc
    17761 gccatatggg caaagataac gacaccaggc atggcgaata aacaggctgg caatcagcaa
    17821 aacggtcacg ctgattaatg tcgcggtccc catatgacga aagaaatcga gcattttaac
    17881 gtccatcacc acgctgtagg gcgacaacat aaaatagtga atcgcctgag cgggcatcaa
    17941 taacgcgata tagataaaaa aactcaacag caaatacttc acgccgcgca gaggaatatc
    18001 cagccagcgg ggaaggacac attgccgacc aaacagtttg ttaccgagat cgccgattaa
    18061 ttcagaaagc gtaccaaccg ggcataacca tgagcaaaag gcctttttga gtaatagact
    18121 gatgacgata aaagcgacca ataacagcat cgcggcggcg tggacggacg gtaactgacc
    18181 tgttacaagg ctatatttca gattcatcag cccggcaatc ggtagccagc cttcgatacc
    18241 tcccggtctg gcgacaaatg tcgtgctact tgccgtttcg taatagcgca cccaatacca
    18301 gaacgtgatg gcaatataaa tattcattgc caacagtaat aattgcgtcg ctttacgcca
    18361 ggtcgtggca ttacgccagt cattccacgg taatttgccg cccgtcgtgc ctggccgccg
    18421 ctgccagcgg gttcttttat tctctgccat gattttgcca gtccgttaag ttgtatacca
    18481 aatgccacta ttctagttgt tcttaactgg ctgatattga ttcaaatcgc gttcaggtct
    18541 ttcttatgca accatgcttc cagagcggca acactgcgtg taatttcttc gtgtggaagg
    18601 ggggcagata atggctgctg ctccagttgt gcgcatagct ggctggcgat atgcattccc
    18661 agactcgagc aactgctttt tagctgatgc gcggcacgct ttattttctc gctatcctga
    18721 ctggcgcggg caatgtcgat ttcatcgaga agcggcaggg catgttgtgt aaataatacc
    18781 agccattcgt ggatcttctc cgtccccatt aactgagcat cttcattgag ttgcgataca
    18841 tccagcgatt gatcattatt gacttgcagt tggagatagt gcgccagtaa ctgaccgagc
    18901 acttcacgcg gcaccggttt agggataatc ccgcggaata atgaactggt acgctggcgc
    18961 agcgtttcgt caatgacatg ggcgctaaag ccaatcaaaa ccagcgacgg atattgctgt
    19021 gccagttgtc gggcaagcgt aatgccgtcg atatccggca gatcaaaatc caccagtgcg
    19081 gcagcaaacg gttcgctatt ttgcagtgtc tctaaagcct gcgcggcatt gccaacagca
    19141 acaatctgcg caccactggt tttcagcatc tcaatggtaa ttcgctgggt tagcgggtta
    19201 tcttcaatta acagcaaacg taaaccgtca agacgcaccg cctgattgac tgtttttggc
    19261 acgggtgccg tggcaacacg taacggcaag cgtaaacaaa aacagcttcc aacctccggc
    19321 gtgctggtgg cgctcagttc gccgcccatc gcctgggcca gacggctact gatagtcagt
    19381 cccagcccgg tgccgccgcg tttgccgctt acctgcacaa atggctggaa gatttctgcc
    19441 agtttcgcgg gatcaatacc gcagccgctg tcttccactt cgaccagcca ttgctcgcca
    19501 tcagtgcgac tacgcaggat aatgtaccct tcgtcagtaa aacgcagggc gttgctcaac
    19561 aggttggtta taacctgacg aatacgtcgt ggatcgccca ttaacgcgca cggcatatca
    19621 tcggcaattg ccgttgccag gcgaatcggg cgacctttca cccgtccgct cattaattgc
    19681 agggtacttt ccagcagcgg gcgcggttca aagggctcat cgctgaccga aacattcttg
    19741 ccacctgctt cgatagcgga ataatcgaga atatcgttga ggatggtcag caacgattca
    19801 ccagagtcag taattgcccg caaatcatca cgctgggcgt taagtgcggg gttatctgcc
    19861 agcagttgag cagtgccgag aataccgtac agcggtgtgc ggatctcatg gctcatcgcc
    19921 gccagaaacg ccgattttgc ctggctggct ttttctgctt ccgcccgtgc ctgtcggtgt
    19981 tctatcacca gttcctgcaa ttcagctgta cgcgctttga cctgcgccgc cagctgttcg
    20041 cggtggcgat tcagtgcatg aacattgctg cgaaacgcat ccatcagccg cccgatggta
    20101 tccagctccc gtacgccagc ggtttccggg aaaggggagt caatatcacc gtccagcagc
    20161 cgttgcagcg cctgcgtttg ttcggcaagt ggacgcgtga ctgagcgata aaccacgcgc
    20221 cagaggatca gaatcagtgc gcaaagtgaa atcatcccca gcaataacag gctgtattgc
    20281 ccgcgtgcac tcgctttttc cagatgcgcc agtccgtgct gattacgcag ctcaatggtg
    20341 tcgaccagct gactaacttc gctactaaac tgcgcgaact gggcgatgtt attttgtgcg
    20401 agagtttgta ggtgattgct gatttcactg tcctgctgat acagcgccag caaatcgcta
    20461 tattggctaa cggtagttaa cgttgttgcg acctgcgcac gaacacccgg atcttcaatg
    20521 cgtatttgcc gacgttgcag aattttcacc gcattattga gctgcttttc cagcgttggt
    20581 gcatttttct ggatctgctc cagccccaga ttcatcacca tttgctgcac ccgcagagcg
    20641 ctaaggcgca gttcattcat ctggttaaca tactcaagat cgatatcaat cagccgatcg
    20701 agtgcacttt cagcagcctg acgctgatct tgttcgatca aatcgtaaat cccggcctgg
    20761 gtcgctccag cggaagttgt cgcattattc gcctgacctt gcgccaggcg tgcgatctca
    20821 tcggcggcag cgactatctg ctgactgagt tgctgttgtt gctggcgcag ttgcaaacgc
    20881 tgccccacca gttccccttg ctgacgtaac gaacgggaga tctcctgctc ctgttgttca
    20941 atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg
    21001 ctttgtgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
    21061 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
    21121 atggtaggaa tcaatgcctg agtgttgtct ttttccacct ggctgataaa gcgcaggttg
    21181 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
    21241 agtcttcggg tcagggttaa attcacggtc ggtgcacttt aggtgaaaaa ggttgagtcg
    21301 caaagcggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
    21361 ctatttttac ttctttccct tttcatgttg ccggcatttt cggctgataa cctgttgcgc
    21421 tggcatgatg cgcagcattt cacggtgcaa gcctctacgc cgcttaaagc caaacgcgca
    21481 tggaaactgt gcgcgcttta tcccagcctg aaagattcat attggttatc gttgaactat
    21541 ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc
    21601 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
    21661 gccattttgc tcggtagtag cacgacctca tttcccgacc tgcaaaagca ggtagcaagt
    21721 ctgccggtga tcgaactggt aaatgctatt gatgctcccc aggtgaaaag ccgcgttggt
    21781 gtgccctggt ttcagatggg ctatcaaccg gggcgatatc tggtgcaatg ggcgcacggt
    21841 aaaccactga atgtgctgtt gatgcccgga cccgataacg ccgggggcag taaggagatg
    21901 gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt
    21961 ggtgataacg atattgaaat ccagcgtaac ctgttgcagg agatgctgga acgccatcca
    22021 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg ggaagggcgt
    22081 aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg
    22141 ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg
    22201 gcggttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
    22261 ccgattttag ttctgacgcc gaaaaatgcc gaccgtgaac atattcgccg ctcgctgtca
    22321 ccagggggat ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct
    22381 tcaccatgtt gcgtcaccag taaatccgcg ctgagtttat gacgtaaacg acgaattaac
    22441 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgtagcaga
    22501 cgttcacggc tgagaatttc gcccggattc gtcacaaatg ccaccaacat ttcatactct
    22561 gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg
    22621 caataaccgg caaagcgata gcagttgtcc tgagtgtgcg gttgagcttg tcgcgcgagg
    22681 tcgattcgcc agagcagatt tttcacccgt actaccagtt cgcgcagttc cagcggtttg
    22741 gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
    22801 ccggtaacca gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg
    22861 ccattttcat cgggtaagtt gatatccagc agaattaaat ctaccgactg attctgcata
    22921 atttcccgta gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag
    22981 taggattgta atcgcgcctg ggtaaccggc tcatcttcaa caataacaat gtgatgtggc
    23041 atcagagggt tttactcatt ctgttcatat ctgttcatat tctgccgtaa gccgttcatc
    23101 ctgaccagtg ccgctgttca tatttgctca ttaagatcgc ttcactaaac cataattcta
    23161 caggggttat tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg
    23221 tactggcgct ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg
    23281 ttgggatcaa agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg
    23341 tgtatgaaga atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat
    23401 gccatgactg tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga
    23461 gcaatgatat ctaccagacc tttattgctc actccattga tacacctgaa aaattcgaag
    23521 ccaaacgcgc ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa
    23581 cctgccgctc ctgccataac tacgatgcga tggatcatgc gaagcagcat cctgaagcgg
    23641 cacgtcagat gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta
    23701 ttgcccacca gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg
    23761 ccagtgctaa tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg
    23821 cgaaaggcga taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta
    23881 aacgtgacgg cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc
    23941 agcgtgtact cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg
    24001 tgcagcagca ggtgaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga
    24061 gcaagttgca ggccaccgca tggatgaaga aaggcgacat ggtgaacgat atcaaaccga
    24121 tctgggccta tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg
    24181 aaatcgccca ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta
    24241 ccagtctcga taaacgtgaa gaacgcactt tgttgaaata tctgcaaatg aatgcgtctg
    24301 acaccgcagg taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct
    24361 ctttcaggca tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat
    24421 gctggggccg tcattgttaa cgccgcgccg tgcaactgcg gcgcaagcgg cgactgaggc
    24481 tgtcatctcg aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgacggt
    24541 gaaggatggt cgctttgtgg cggtaaaacc gttcgaactg gataaatatc cgtcgaaaat
    24601 gattgccgga ttgccggatc acgtacacaa cgcggcacgt attcgttatc cgatggtacg
    24661 cgtggactgg ctgcgtaagc gccatctgag cgacacctcc cagcgcggtg ataaccgttt
    24721 tgtgcgcgtg agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtgca
    24781 gaaaactcac gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt
    24841 ccataacgct tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac
    24901 gggcggagat tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtgg ttggctcaat
    24961 ggaagtgtat gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt
    25021 gctgtggggc tccgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga
    25081 tgtttatgaa tattacgcgc agttgaaagc gaaagtcgcc gccggtgaaa ttgaggtcat
    25141 tagcatcgat ccggttgtta catccaccca tgagtatctg ggacgcgagc atgtgaagca
    25201 cattgcggtt aacccgcaaa ctgacgtgcc gctgcaactg gcgctggcgt atacgctgta
    25261 cagtgaaaac ctgtacgaca aaaacttcct cgctaactac tgtgtaggtt ttgagcagtt
    25321 cctgccgtac ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa
    25381 actgaccggc attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag
    25441 aacgcaaatt attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg
    25501 gatgattgtg gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggctttgg
    25561 ttttggctgg cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttctgagtgg
    25621 tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca aaggctacag
    25681 cagcacaatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa
    25741 ctggaacggt aaatcggtaa aactgccgca gctgaaaatg tgtatttttg ccggaactaa
    25801 cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac
    25861 ggttatcgcc atagataacc agtggacctc aacttgccgc tttgccgata tcgtgctgcc
    25921 tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact ccaaccgtgg
    25981 cattatcgcc atgaaacagg tcgtgccgcc gcagttcgag gcgcgcaacg actttgatat
    26041 tttccgcgag ctgtgccgtc gctttaatcg cgaagaagcc tttaccgaag ggctggacga
    26101 aatgggctgg ctgaaacaca tctggcagga aggtgtacag caaggcaaag gacgcggcgt
    26161 tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt ttgaccatcc
    26221 gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac cgctgggcac
    26281 gccgagtggc ctgattgaga tctattcgaa aaccatcgcc gatatgaact acgacgattg
    26341 tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ctggctcgca
    26401 gacgtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact cgcagttatg
    26461 tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag tgttcattaa
    26521 cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg tatttaacgc
    26581 tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg gcgtggcgcg
    26641 aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg cgctgtgcaa
    26701 atacggtaac cccaacgtgc tgaccatcga catcggtact tcgcagctgg cgcaggcgac
    26761 cagtgcgcac actacgctgg tggaaattga gaagtacaac ggaacagtgg agcaggtgac
    26821 ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg cgtcgcaggt
    26881 gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg gctggcgcag
    26941 ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggct
    27001 gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa cgagctggaa
    27061 aaccgtattg ccacgctgac agtacgtgac gatgcccgtc tggaactggc cgcggacttt
    27121 tgcggcctgt ttctgatgac cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa
    27181 caggacgagc aagagattaa acgcttgtta gttgaggcag ggatggaaac cagcggcaat
    27241 ttcaacgaac cggcagatca tctggcgatc tatctcgaat tgctcagcca tctgcatttt
    27301 tcgctgggag aggggaccgt tcctgcgcga agaatcgaca gtttgcggca aaaaacactg
    27361 acggcgctgt ggcaatggtt accagagttt gttgcgcgtt gtcgtcagta tgacagcttt
    27421 ggtttttacg cggcactaag ccagttattg ctggtgttag tggagtgcga ccaccaaaac
    27481 agataacgtc gtttgtgcgc ctgaaaagac gcgtttagcg tcgcatcagg cattatggcg
    27541 cagttgccgg atgcggcgtg aacgtcttat ccggcccaca ggaactgtaa tctttgtaga
    27601 ccggttaaga tgcgtcatcg catccggcaa acacacatca cggatgagct acaaaccggg
    27661 aaagccgctg gcgcagcagg cggttttcct gcttcaggtg cgcaatatca tccattaacg
    27721 tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg cgtaccgcgc
    27781 gttgcaccac aatggcggca tggtcgtcaa atacccaggt tgtttcctgg atctcacgcg
    27841 gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg ccggtatgca
    27901 ggcaaaattc ggtaatagta aaagtcaccg taacattagc cattatgctt tcccccaatc
    27961 tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg cggcagtgtt
    28021 ttcatccggt ttcggcggca tcacgatttt tagtaccgca tacagatcgc cggtctgttt
    28081 tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc tgcctggcgg
    28141 gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagcac ccagcgccgc
    28201 ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat caaacagcgg
    28261 atgtggcgca atatgaatta ccagccacaa atcgccattt ggaccgccgt tttcgcccgg
    28321 cgtcccctgg cctttcagac ggatgcgttg accattgccg acgcccgccg ggatcttcac
    28381 attcagcgtt ttcggaattt cctgttcgat catgccaaag gcgttataaa ccggcaggtt
    28441 atagctgatg gtacgcttat gctcagtaag cgtttcttcg aggaataccg ccacttcgat
    28501 ttcaatatcg tggccgcgtg tggcggggcg ttgacggctc tggcgggcat gctgaccgaa
    28561 aattgacgag aagatatcgt caaaatcttc ggcgttaaaa ctctgaccgt cgccatggtg
    28621 gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat actcagcgcg
    28681 acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc gggcttcggc
    28741 atccggttct ttgctgacat caggatggta tttgcgggca agtcgacgat aggcggtctt
    28801 gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat cctttaattc
    28861 catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag gtaactccta
    28921 taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta ttgaaatcat
    28981 cgcggagagg aggtcgccat caagatgggt tgctgaacat attttaaaca ggtgaaaaag
    29041 ggtgagcgat ttttgatagt tgaaccaggc actttaagtt taactagggc gtcattattt
    29101 attaaatttt atagacgcta tatatgggta gtaatataca tggaattagt tgcactgcaa
    29161 ataattattt gaaacaggcc tggaacgata taaaaaatga gtacgaaaaa aatcaaacat
    29221 attcaatcac gctttttgaa aacacactgg tgtgttttat gcggttatac aatgaactca
    29281 gacgtaaagt aaatgaagag gatactccat gtctggaatg tgaatcacta gaaaaagaat
    29341 ttgaggaaat gcagaatgat aatgatctat cattatttat gagaacattg cgtactaatg
    29401 atacacaaat ttattcaggg gtttcaggag gtattacata tactatacaa tatgttcgag
    29461 atattgatat tgttagagtg tccttgccgg gcagagcttc agagtctatc acagatttta
    29521 aaggttatta ttggtataac tttatggagt atattgaaaa cattaatgcg tgtgatgatg
    29581 ttttttctga gtattgtttt gatgatgaaa atataagtgt ccagccagag cggataaata
    29641 cgccgggaat atctgatttg gattctgaca ttgatttgtc tggtatatct tttattcagc
    29701 gtgaaactaa ccaggcatta ggattaaaat atgctcctgt agatggcgat ggatattgtc
    29761 tgttaagagc tatactggtt ttaaaacaac atgattattc atgggcgctg gtcagttata
    29821 agatgcaaaa ggaagtttac aacgaattca ttaaaatggt tgataaaaaa acgatcgagg
    29881 ctcttgttga tacggcattc tataatctca gggaagatgt aaagacgtta tttggcgttg
    29941 atctacaatc tgacaaccaa attcaggggc agagtagtct tatgtcatgg agttttctgt
    30001 tttttaaaaa caattcattg atagttgctt gaataacgaa aaatgtatcc tgcatttacc
    30061 cgagtttata tttaatgata acaagaactt gcttgcttta gataccgaca cgtcggatag
    30121 gattaaagcg gtgaagaatt ttcttgctgt tctttcagat agcatttgct cattatttat
    30181 tgttaatagt aatgtggcat caatctcctt ggggaatgaa tccttttcaa cagatgaaga
    30241 tcttgagtat ggttatttaa tgaacactgg caatcattat gacgtttacc tccctcctga
    30301 actttttgct caggcttaca agttaaacaa taaggaaatg aatgcgcaac tcgactattt
    30361 aaatcgttat gcaatttaat ggcaaaggca tatgctaaaa accattgtta ttagtctcac
    30421 acttttttat tggtaaatat tgtctctgta ttggtaacgc cgcagatatt ctgtttagcc
    30481 acaggtgcaa ttatcagcgg cgtacgcgag gcaggggcta atcaggcata gtttgcgtca
    30541 aaccttgcct gtttttgaag atgtatatag aaaaacaggc gttcaacaag ccattttgcg
    30601 aacctgttcc cggaaaaaag tcatatttct gtcacactct ttagtgattg ataacaaaag
    30661 aggtgccagg aatgaacaaa acgctaatcg ccgcagctgt ggcagggata gttttactcg
    30721 cttcaaacgc tcaggcacaa accgtaccgg aaggctatca gctacagcaa gtgctcatga
    30781 tgagccgcca taacttacgt gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga
    30841 cgccgaataa atggccagaa tgggacgtcc ccggtgggca actcaccacc aaaggtggcg
    30901 tgctcgaagt gtatatgggc cattacatgc gtgaatggct ggcagagcag gggatggtga
    30961 aatcggggga atgcccgccg ccgtacaccg tttatgccta tgccaatagt ctgcaacgta
    31021 ccgttgcgac cgcacagttc tttattaccg gcgcattccc ggggtgtgat attcctgtgc
    31081 atcaccagga aaaaatgggc actatggacc caacctttaa cccggtgatc accgatgatt
    31141 ccgccgcatt cagtgaacag gcggtggcgg caatggagaa agagctcagc aaactccagc
    31201 ttaccgacag ctaccagcta ctggaaaaaa tcgttaacta taaagattcc cctgcctgta
    31261 aagagaaaca acagtgttcg ctggtggatg gcaaaaatac ctttagcgcc aagtatcaac
    31321 aagaaccagg tgtttccggg ccgctgaaag tcggcaactc gctggtagat gcgtttactt
    31381 tgcaatatta cgaaggtttt ccgatggatc aggtggcctg gggagaaatc aaatctgacc
    31441 agcagtggaa ggtgttgtcg aagcttaaaa acggctacca ggacagcctg tttacctcac
    31501 cggaagtggc gcgcaatgtt gcgaaaccgc tggtcagtta tatcgacaaa gctctggtca
    31561 ccgatcgcac cagcgcaccg aaaattacag tgttggttgg gcacgactcc aacattgcct
    31621 ctctgttaac ggcgctggat ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc
    31681 cgattggcgg caaaatcgtt ttccagcgtt ggcatgacag caaagccaat cgcgatttga
    31741 tgaaaattga atatgtgtat cagagtgcgg aacagttacg taatgccgat gcgttaaccc
    31801 tgcaggcacc tgcgcagcgt gtgacgctgg aattaagcgg ttgcccgata gacgctgatg
    31861 gtttctgccc gatggataag tttgatagcg tgttgaatga agcggtgaaa taacagaaaa
    31921 ctcccccgcg agaagcgggg gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc
    31981 gccccaaaaa agcgaatctt tatcggtctt agcaaaggct ttgactaaca cttcatcact
    32041 accttcttcc caaatctttt ccgccatttt ttcgtcgtac ccggcgactt cgaaaatggc
    32101 ctcggctatt tccggcgacg tattgcgcag agatgcccat tcaccgacgt gatgagcttt
    32161 cgcttcttga gttggcatgc gtatcctcct gttgaagatt agccgttaag tttaactgcc
    32221 agacctgcga catattcccc ttgataacga gcaatagaca gttcttcctg gctgggctgg
    32281 cgtgaaccgt caccgcctgc gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt
    32341 gaaacgtcaa ataattcctg cgctgcgtag ccaataggga caattaccat gccgtgatgc
    32401 gcaagggtcg tccaggttga agtaatagtt tgttcctgac cgccgccagt accggtggaa
    32461 ctaaagacgc tcgccagttt tccgtatagt gcgccggaag cccacaggcc gcccgtctgg
    32521 tcgaggaagg tacgcatttg accggacatg ttgccaaagc gggtaggtgt accaaaaata
    32581 atggcgtcgt aatcggccag ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg
    32641 cctgcttttt caaataattg cggcggcatg gtttccggta cacgcttaac gacaacttct
    32701 gcgccatcca ctttgcttgc accctcagcg actgcgcgtg ccatcgtttc aatatgtccg
    32761 tacatggaat aataaagcac cagaacttta gccatttcta accactcctc gtgttatctc
    32821 tattccgtag cgattcgcta ccacttattt aaagataaga cgtccttttc agagtgcaaa
    32881 tttcacaacc acttatttga tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa
    32941 tgacacattt ttatctcatc gcgttttttt aatcataaga gcggcttatg gataattatt
    33001 ggagatgata tctattctcg ctaagaagct gttgcaggat attaccaaac gcgggtctgc
    33061 ccgcgtcagt tcactaagct tagtcccacg tagcgaaaat atggcagccg ccatacgccg
    33121 cgttaattct atgcaatatg atgtctatac ccagacggag gtcagtaatg gcaaaccatc
    33181 gaggcggttc cggcaatttt gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag
    33241 gtggacagca cagcgggggt aatttcaaga atgacccgca gcgcgcatct gaagcaggta
    33301 aaaaaggtgg taagagcagt cacggcaaaa gcgacaacta gccgggctaa tcaatgacga
    33361 atgcattttt gtctgtagct cgtcaaaaag ccatcaccgc cggttacccg gtggttgata
    33421 ctgatgacaa atgtaagctt gcctgatgcg cgatgcttat caggcctacc agaagattgc
    33481 aatatattga atttgcactg ttttgtaggc cggataaggc gtttacgccg catccggcat
    33541 gaacaatgcg tacgttgtca acaatctgca ccgccgggta atcccggcgg ttttcttttt
    33601 atggctcctg atgaacaact tctggcggtg gaacgtcaac caaccttctg cttaataacg
    33661 cattgagtaa aatcgcgcca aaggttgctg taccaatccc tcccaacgta aaaccgccca
    33721 gcgtgagagc aaaatcaccc gcgcccagca ctaaggttac tgcgaccata atcaaattac
    33781 cgttctggct taaatcgaca cggttttgta cccagatcct tgcgcctgcg acggcaatca
    33841 gcccgaacac aacaattgat gcaccaccaa tcaccgcggc cggaatggta tgaatcagcg
    33901 cgccaaattt cggtgaaaag cccaacagca tggcgatgac ggcagcagcg acaaacacca
    33961 gcgtcgagta gacttttgtc acggccatta caccgatatt ctcggcgtag gtggttacgc
    34021 cgctgccacc gacagaacca gaaagcatcg tcgccagccc atcaccgacg aatgcccgtc
    34081 ccatatatgg atccatattg cgtccggtca tcccggcgac tgccttgaga tgacctaagt
    34141 tttccgccac cagaatcacc gccacgggcg caatcagcat cattgcctga ccattaaaag
    34201 caggagtgga aaaatgtggc agaccgaacc aggcagcatg gctgacgaga gtataatcga
    34261 cggcttttcc cagccctaaa acgttggtca tcacgccata cagcagacag gcgacaatta
    34321 atcctacgag aatcaataac cgctggatca tgccacgggt aaacaccgcc accagcccaa
    34381 tacacagcac cgtcattacc gccatccagc tatcaaaggc cgaagccgat acacttttca
    34441 ctgcgatagg cgctaagttc aggccaatcg ccatcaccac cgcacccgtc accaccggcg
    34501 gcattagccg ttcaatccag cgtgtaccaa ttttcatcac caccaggcca atgacggtat
    34561 aaaccagccc acaggcgata atcccgccca gcgcgatgct gatattcggg ttcatgccct
    34621 gaccgttaaa gcccgtcgcg gcgatcacca cgccgacaaa agccgcgctg gagccgagat
    34681 aactggggac gcgcccgccg gtaataaaga aaaacagtaa cgtgccgatc cccgacatta
    34741 aaatggaaag attgggatcc agccccatca gaatcggcat taacaccgtc gcgccaaaca
    34801 tcgccaccgc gtgttgaacg cccattattg ccgtctgagc aaacggcaat cgttcatccg
    34861 gcgcgaccac gccgctctct gtagaggtcg attttaactg ccagtgagga aaaccgaaca
    34921 ttgccatcag ctgtctcctt aaggaggtta acaagcaggg cgcatcagcg cgtgataact
    34981 gcgatcgaac cacaccagcc cgtagggtgt ggcgtgacga tgaatcgctt cgatggcaca
    35041 aaacaaaatg tcgtgggtgc cgacgctcac cacctggctg atacggcagt caaacgaaac
    35101 cagagcctct tccagttgcg ggcatccggt cacacccgtc tgccagcggg cggcggcaaa
    35161 gcggtgttcc atgggcgttt tgccgccaaa aagatttgaa agtggctcct gcccggcgct
    35221 aagtgtattt acacacagcg ttcgattttc attgaatacc ggccagacgg acgccccacg
    35281 attcaggcac accagtaatg tgggcggcgt gtcggtcacg ctgcagaccg cgctggcggt
    35341 gaacccggcg cgcccggctg gaccatccgt ggtgatgata ttgaccgccg cgcccataca
    35401 ggacatcgca tcgcgaaagg tttgttgatc aactatgttc atagtttgct ccttacaaca
    35461 gcccgcaggc ttcttcaaag gacagacgtg gcaggcgcgc ataaagtttg ctgttatcgc
    35521 catagccgat gttaatcagc agattgctct tcagcgtgct gcctgcaaaa aaagcgtcgt
    35581 caacgtattg acggttaaaa cccgacatcg ggccggtatc cagccccagc gcacggcagg
    35641 cgacgatcag ataagccgcc tgcatggaac tgttgcgaaa cgctgtttct tcggcaagtt
    35701 gtgggctgga ggtaaaccaa ctgcgggcat caccgtgggg aaacagtagt ggtaaccgtt
    35761 cataaaattc actgtcccag gcgacgatag cggtgacggg cgcggtcagg gttttttgca
    35821 gattgccgct ggaaagcgcc gggcgcagac gttcttttcc ttctgcagtg cggataaaca
    35881 cgatccttgc cggagaacag ttagctgatg tcggccccca tttcatcagg gcataaagct
    35941 cccgtaacgt ctcatcgctg acgggtgtct cccgccagcc gttgtgagtg cgggcatcgg
    36001 tgaacagggt gctaagcgca cctgggctaa cggcttcgtt catagcaatt ccttacaggg
    36061 cggcttcacg gtgatgtaac aggctggcaa gcccgttgag taacagagca ttaaacgttt
    36121 cgggatcggt cacgttgcag gcgtgtccgc catagcgcat caccattttc tggctatcgg
    36181 gcagggcggc atgaagttca ctggaacatg ctgatggcac cagcagatca tcactggcgc
    36241 agatgatttg catcgggcag cggatgcgat ccgcatggtg actaaagtca gcgcgtttga
    36301 gggcgttaag tcgacgcagt aaattatttt tgccctgaaa atgcgccagt gccagcgcgt
    36361 cttctgcctc aaggcgaggt gcgcgggccg ccatccagtc ggcgggatag aggaacaacg
    36421 gctgcgcttc cacccatgcc tgcgcgccgc cgctatacag taatcgttcg cgaacctgaa
    36481 aacagcggcg cgtatgggcg tttattcgta gccagccgtt aacgctgacc agcacagtta
    36541 ccgacgcggg ataatccagc gccagctgca ttcccaccag cgcaccgagc gcatggccga
    36601 ccaccgcgta acgctcaatc cctgcggcta ccagtgcctg atgcagttct gctgccatct
    36661 gggcgatact gtaatcttct gccagcgtgt cgggattatt gccggtaccg cgctggtcgt
    36721 aacagactac ctgatactcc tgctccagca ctgccagttg cggtaaccag taactgccgc
    36781 taccgccaag acccgaaatc aacaccacta cgggcgcatc agcataaggg ggaggtgaga
    36841 gtgaaagttt catcgcggcc tcacttggcg atatgcgcaa ttgtggcgat ttccaccagc
    36901 gcgtcaggtt ttaccagtcc gcactgaatg cagaatcgcg ccggtttatc acccggaaaa
    36961 aactcggcgt agatttcgtt aatcgcggcg taatttttcc agtcggtaat aaagatgctg
    37021 ttgaaggtca catccgccat cgtgccaccc gccgtctcga tcaccttgcg gatggtttcc
    37081 agaacgtggc gggtttgcgc ctttgggtca tcggcaaaca gcatgttatt atgttgatca
    37141 aaagccagcg taccggagac atacaccact ccatcagcca gcgtgccggg aacgaagggg
    37201 gccagcggtg cgctgctgcc agcgggaata attacggatt ttggcatcgt taaactcctt
    37261 aagcgatacg agcaaaggac gtgggagaaa gcgcgtcgca gaacgtttcg acgtcgctga
    37321 cccagccaaa aaaggtttcg atattgaaca acgcagcttt ctgcgcaaat tccggccccg
    37381 cctggtgagt tgcgtcttcc agcaccacgc cgaaatactc cagaaaaaag ccgtcgcgta
    37441 gcgtcgattc gacgcagacg ttggtagcga taccggtgaa aaccagatgg cgtattccac
    37501 ggctgcgcaa aatgctgtcc agcggcgtat tgaagaaacc gctgtagcgc ggcttcggca
    37561 gcacaatatc gccaggctgc ggcaccagtt catccaccag ttgataatcc caggagcctt
    37621 tcgccagcaa tttcccctgc agctgcggct gcttacgcat ggttttcagg gcgttcgatt
    37681 tatgaaaatt cggtgagccg ggtccgccag cttcgacata ctgttcatcc cagccatttt
    37741 gaaaccagat gatcagcatc cctgccgctc gcgctgcggt cacggcggtt tgaatgttgg
    37801 caatgaccgg gcgagtggtt gagacatcaa acccggcgag atctaagtag ccgcctggcg
    37861 tggcataagc gttttgcata tccaccacga tcagcgcact ttgctgcgga tcgaaggtaa
    37921 tggcttccgg tcgagcggtt aaggtcgtca tcatgccacc tcctgagtca gcgcagggag
    37981 atgggcgcgg cactgcatca gtggttgaat gcgctcgccg aaggtttcga ttcccgacag
    38041 aaaatcatcg aaggttaaca gcacgccttc ggcaccaggc acgcttgcga cttcatctaa
    38101 catgcgcgcg acactggcgt aagaaccgac taacgtcccc atattgatgt ttaccgccga
    38161 agtgggatcg gccatctgac ggacgttggt atcagtaccg gagcgggtat ctttctgact
    38221 ttgttcggtt agccagctta acgcctcttc atccgcgccc gctttgtagt gttcccattt
    38281 ggcgcgagcg gcatcgtcgg tttcatcggc aatcaccata aacaatacat aagagccaac
    38341 gtcgcgcccg gtttgctctg cggcctgttt catccgcaca gcggtcgggg cgaaagccgt
    38401 gggtgtattt acacctttgc cgaaacagaa gttgaaatcg gcatactggg cggagaacgc
    38461 catgccagcg tcgctttgcc cggcgcagat cactttcatg gggacactcg gttgcggact
    38521 gacgcgacaa tcattcatgg tgaaaaaatc gcctttaaaa tcgcttttcc ccgtgcccca
    38581 caggtcgcgc agcacctgaa catattcggt gagatagtcg taacgacggg agaaatagtc
    38641 atcgccaggc cagataccca tctgctcata ctcgggcttt tgccagccag tcacgaggtt
    38701 gacgccaaaa cgcccgccag agatggagtc gatggttgcg gccatacggg cgacgattgc
    38761 cggaggtaac gttaaggtgg cagctgtcgc gtaaatctga atgcgcgagg tcacggctgc
    38821 cagccccgcc atcaaggtga acgactcaag gttgtgatcc cagaactcag ttttgccgcc
    38881 aaagccacgc agtttgatca tcgacagggc gaaatcgaaa tggtagtgct ccgctttttg
    38941 cacaatggct ttattcagtt caaaggtcgg catgtactgc ggcgcgtggg tcgaaatgag
    39001 ccagccgttg ttgccaatag gtacgaatac gccaattttc atcatcaacc tctcttcgtc
    39061 tcgtaaagtg aaagtcagac ggggcgctgc atcctgcata tccttttcag ccgcgtattg
    39121 gcttgtttgc aaagcggatg ccagttttta aaaagtgaat gttattaatg tgttaatgtt
    39181 tggttatata aaatatctgg aaaaaagtgg actaaacggt caaaacagtt gcacataaaa
    39241 catgcatccg tgcgcgatga gagtgcagaa ggtcgaggcc gggcgggggt tttgctatcc
    39301 tgttgccaat ctacaagagg ggagagcgca tgacgcaagg cgcagtgaaa acaacgggta
    39361 aacgttcgcg cgcagtaagc gcgaagaaaa aagcgattct tagcgcagca ctggacactt
    39421 tttcacaatt cggttttcac ggcacacggc tggagcagat cgcagagttg gcgggtgttt
    39481 caaaaaccaa tctgctgtat tactttccgt caaaagaggc gctgtatatt gccgtgctgc
    39541 ggcagattct cgatatctgg ctggcaccgt taaaagcgtt tcgtgaagat ttcgccccgc
    39601 tggcggcgat caaagagtac atccgtctta agctggaagt ctcacgcgat tatccgcagg
    39661 cttcacgtct gttttgtatg gagatgctgg caggcgcgcc gttgttaatg gatgaactga
    39721 cgggcgattt gaaggcatta attgatgaga aatcggcgct gattgccggt tgggtcaaaa
    39781 gcggcaaact cgcgccgatt gatccgcagc atttgatttt catgatttgg gcttccactc
    39841 aacattacgc cgatttcgcc cctcaggtgg aggcggtgac aggcgcgacg ttgcgcgatg
    39901 agatattttt caatcaaacg gttgaaaacg tgcagcggat tattattgag gggattcgac
    39961 cacgttaaag atgccggagg aggttgtaac atcctccggc tacctgttta accgatcgtc
    40021 attaaactgg cgttaccgcc agcggcagcg gtattcacac tcagcgaacg ttcgatatac
    40081 agccgttcca gaaggatatt gctttcgcca cgggcaaaac cctgcaccga aacaattgcg
    40141 ccatcccgcg cagcaacggc ttcacacaat gcgcgaagct gatccgaatc accgtggaag
    40201 atcaccgcat caaagggttg agcggttata ttttccgctt tcgccagttg aatacgttcg
    40261 ctgactgccg atggcaatgc ctttactagc tgacgatgca gcgcgtcatc cggccacagt
    40321 acctggctgc ccaccgccag cacggcggcg agctgagtca gcgcatcctg ctcatcatcg
    40381 gcaatacaca acacgcgctc acgcggcagc agcgtccagg tgttgcgttc acccgtcggc
    40441 cccggcagca atcgttgtgt tcctgcctgc gccagctcgc catattgcgt acataacgcc
    40501 tgcaattctg gacgatttgc tgcccattcc cgcagtgcat ttagcggctg agtcaatgcg
    40561 gctttcaact gtgcatcgac cggatactct gcatcctgac gcgcgagcgt cactgccagc
    40621 gcactttccg ggcgattcgc cagcagacgg tagagataga gcggaccgcc tgctttcggc
    40681 ccggtaccgg acaacccttc gccgccgaat ggctgcacac ccaccactgc gcccaccata
    40741 ttacggttaa catacaggtt accaacatgg gccgagccag tgacctgggc gatggtttca
    40801 tcaatacgcg tatggacacc aagcgtcaga ccatagccgg aagcgttaat ctgctcgatc
    40861 agctctggta gctggttacg gttgtaacgc accacatgca gcaccggacc aaagacctct
    40921 ttttgcaatt cggcaaagtc atccagttcg atcagcgtcg gggcgacaaa ggtgccgctt
    40981 tgccattcac gggcatcttc gctgttttcc cgcaccgcct ggaacaccgg acggcctttg
    41041 ctacgcatgg tctgaatatg gcgctcaata ttggctttcg cttcgctatc aatcaccgga
    41101 ccgatatcgg tggtcaggcg acccggatta cccatccggc attcggccat tgcgccgcgc
    41161 agcattttca acgtgtggtc ggcaatctca tcttgcaggc acagcacgcg cagcgccgaa
    41221 caacgctgac ccgcactgtc gaacgccgag gccagtacat ccacgacgac ctgttcggtc
    41281 agtgctgaag aatcgacaat catcgcgttc atgccgccgg tttcagcgat gagcggaata
    41341 gggcgaccct gagcgtccag gcggctggcg atattgcgct gcagtaacgt agcgacttcg
    41401 gttgaaccgg taaacatcac cccgcgcacg cgatcatcac ccgtcagttg cgcgcccacg
    41461 gtttccccct gacctggcag caactgcacc acgcctggcg gtacacccgc ttccagcaaa
    41521 atggcgatcc cttgcgcggc aatcagcggc gtttgttctg ccggttttgc cagcacgctg
    41581 ttacctgccg ccagtgcggc agcaatctgc ccggtgaaaa tagccagcgg gaagttccac
    41641 ggactgatac acaccacagg ccctaatgga cggtgggttt cgttagcgaa atcatcccgc
    41701 acctgtccgg catagtagtg gagaaaatcg accgcttcgc gcacttcggc aatggcgtta
    41761 ctgaaggttt ttccggcttc acgcaccaga ataccaatca gttgctgcat ctggctttcc
    41821 attagcacgg cagcgcgatg taaaattgcc gcgcgttcag ccggaggagt ggcaaaccag
    41881 attggcgcgt tattaaccgc actttccagc gcctgttcta cttcacgcgg cgtggcttca
    41941 cgcacatagc ccacaatatc tttcggttct gcagggttaa taacgggcga catctcacct
    42001 gccgctaccg gttgttccag cattggcaag gcctgccatt tttgcagtgc actattgagc
    42061 agggcagaag agagcgaggc cagacggtgt tcgttagcga gatccagccc tgccgagttg
    42121 tcgcgcccgt gaccatacag atcgcgcggc aggggaattt tcggatgcgg taatccagtt
    42181 tgcccttcct gttgcgccag tttttctaca gcagtgaccg gatcggcgac cagttcatcc
    42241 agtggcaaag aggtgtcggc aatacggtta acaaacgagg tgttagcacc gttttccagc
    42301 aggcgacgca ccagatacgc cagcagcgtt tcatgtgtgc caaccggggc ataaatacga
    42361 cacggacggt taagtttgcc gtcggcaact ttcccggtga cctgctcata cagtggctcg
    42421 cccataccat gcaggcactg gaactcgtac tgacccggat agtagttctg acctgccagt
    42481 tgataaatcg ccgccagcgt atgggcgttg tgcgtcgcga actgcgggta gattagattt
    42541 ggcaccgcca gcagcttttt cgcacaggcg agatatgaaa cgtcggtata caccttgcgg
    42601 gtataaaccg gatagccttc aaggccgtcc atctgcgcac gcttgatttc actatcccag
    42661 tacgcgcctt tcaccaggcg aatcatcaga cggcgacggc tgcgggtggc gagatcaacc
    42721 agataatcaa tcaccaacgg gcagcgtttt tgataagcct gaataacaaa accgatgccg
    42781 ttccagcctg ccagttccgg ctcgaaacag agtttttcca gcagatcgag ggagatctcc
    42841 agacgatcgg cctcttcggc atcgatgtta ataccaatat catactgacg cgccagcagg
    42901 gtgagtgatt tcagtcgcgg gtaaagctct tccattaccc ggtcatactg ggcgcggcta
    42961 taacgcggat gcagcgccga cagtttgatt gaaatgcccg gcccttcata gatgccacga
    43021 ccgttagacg ctttaccgat ggcgtgaatc gcctgctgat aggaaaccat atacgcctgt
    43081 gcatctgcgg cggtcagcgc ggcttcgccc agcatatcgt aagagtaacg gaaacctttc
    43141 tcttccagct tgcgggcatt ggctaacgct tccgcgatgg tttcgccagt gacgaactgc
    43201 tcgcccatca ggcgcatcgc catatccaca cctttgcgga tcagcggttc accgctttta
    43261 ccgataatgc ggttcagcga gcgggagagg ctggcttcgt tatgggtgga aaccagtttg
    43321 ccagtaaaca gcagccccca ggtggcggca ttaacaaaca aagacgggct acgaccaatg
    43381 tgtgattgcc agttaccgtt gctgattttg tcgcgaatta acgcatcacg ggtggctttg
    43441 tcgggaatac gcaacaacgc ttccgccaga cacatcagcg ccacgccttc ctgcgatgac
    43501 aacgaaaact cctgcaataa cccctgaacc atacctgcgc gaccactggc gtttttttga
    43561 ttacgcagtt tatcggccag ttgatacgcc agtttgtgcg cctgttcagc aactggctgc
    43621 ggcaggcggg cttgttccag cagcatagaa accgcttcgg tttccgggcg gcgataggcc
    43681 gctgtgatcg cggcgcggga aaccgactgg ggcaaaattt gctcggcaaa gtcgaggaat
    43741 ggttggtgtg gttcctctgc cggagtcggt gcttcatcgc tctcattggc agcgccagaa
    43801 agcagcgcag gtagctccgg cagagtatcg ctgttttcca gttgttcgag ataagaaaaa
    43861 atcgcctgct taattaacca gtgtggcgtg cgatcgatac gtgtcgcggc agacttaata
    43921 cgctcacgcg tcgcgtcgtc cagcttaacc cccatggtgg tggttcccat gccattactc
    43981 ctgttgttct gaaaggtgca acttaacgtt atcgtgaaat atccatgatg ttgcaacttt
    44041 gtgcaactat gttaaatgtg acctgcgtag caagcttaaa aatgaatgaa atgttaataa
    44101 aagaaatcga tatgacaggg attaaaaaaa taactcagac tttttctctg cggcagttaa
    44161 catttttgaa aggtgcaacc gcaaaaaatg tgagagagtg caacctgatg aaaaatagtg
    44221 tcgctgagca ctaaaattta atgtaaatgg tgtgttaaat cgattgtgaa taaccagcgc
    44281 ttccggcagg atacggtcgc cctggtaaaa cataaactct gttaccccgt tccggtggca
    44341 gatataacgg caagtttcga cattgccgat aataattttt tggagacttt agatggctat
    44401 tagcacaccg atgttggtga cattttgtgt ctatatcttt ggcatgatat tgattgggtt
    44461 tatcgcctgg cgatcaacga aaaactttga cgactatatt ctgggcggtc gtagtcttgg
    44521 gccattcgtg acggcattat cggcgggtgc gtcggatatg agcggctggc tgttaatggg
    44581 gttgccgggc gcggtttttc tttccgggat ttccgaaagc tggatcgcca ttggcctgac
    44641 attaggcgcg tggatcaact ggaagctggt ggccgggcgg ttgcgtgtgc ataccgaata
    44701 caataataac gccttaacat tgccggatta tttcaccggg cgctttgaag ataaaagccg
    44761 cattttgcgc attatctccg cgctggttat tttgctgttc ttcaccattt attgcgcttc
    44821 gggcattgtg gcaggcgcgc gtctgtttga aagtaccttt ggcatgagtt acgaaacggc
    44881 tctgtgggcg ggagctgcgg cgacgatcct ttacaccttt attggcggtt tcctcgcggt
    44941 gagctggact gacactgtac aggccagcct gatgattttt gccctgatcc tgacgccggt
    45001 tatcgtcatt atcagtgtcg gtggctttgg tgactcgctg gaagtgatca aacaaaagag
    45061 catcgaaaac gtggatatgc tcaaagggct gaactttgtt gccattatct cattgatggg
    45121 ttgggggctg ggttacttcg ggcagccgca catcctggcg cgttttatgg cggcagattc
    45181 tcaccacagc attgtccatg cgcgtcgtat cagtatgacc tggatgatcc tctgcctggc
    45241 aggagcggtg gctgttggct tctttgggat tgcttacttt aatgagcacc cggcggtagc
    45301 aggcgcggta aaccagaacg ccgagcgcgt gtttatcgaa ctggcgcaaa ttctgtttaa
    45361 cccgtggatt gccgggattc tgctgtcggc aattctggcg gcggtaatgt caaccttaag
    45421 ttgccaactg ctggtgtgct ccagtgcgat taccgaagat ttatacaaag cgtttctgcg
    45481 taaacatgcc agccagaaag agctggtgtg ggtagggcgt gtgatggtgc tggtggtggc
    45541 gctggtggcg attgcgctgg cggcaaaccc ggaaaaccgc gtgctgggct tagtgagcta
    45601 cgcgtgggca ggctttggcg cggcgtttgg tccggtggtg ctgttctcgg tgatgtggtc
    45661 acgcatgacg cgcaacggtg cgctggcggg gatgatcatc ggtgcgctga cggttatcgt
    45721 ctggaagcag ttcggctggc tgggactcta cgaaattatt ccgggcttca tcttcggcag
    45781 tatcgggatt gtagtgttta gtttgctggg taaagcgccg tcagcggcga tgcaaaaacg
    45841 ctttgccgag gccgatgcgc actatcattc ggctccgccg tcacggttgc aggaaagcta
    45901 agggacttag cctgcggcgg ttttgtttgg cttcagcagc gggttgcgct cccttaatgt
    45961 gcctcgccat ataaattgaa tggtgcaggg agcgcgcagg gggcggccaa tcgccgcggc
    46021 cccctgcacc ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc
    46081 ccttcggcta ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat
    46141 ccatgtgggc tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccc
    46201 gcgctgttct tatgttccgg gcaaaaagtt agatttgata atcgaggatg gacgaaattg
    46261 cttgatacac ccgcttatca gttttacatg gaagttttga tgtattgagt caggacggtt
    46321 ttgtcggctg gatacggcgt ttacgcggca tccggcaaga acacatggct cgttgtaaac
    46381 aatcccatct ttgtacctgg aataatcgtt tatatcccct ggcattacct ctctttgttt
    46441 acatttcaac atcattttat aaacattccg cttgtatttt tctttaccgt aatgataatc
    46501 gctatcactg cgatttactt ttctttgcat agattgactc agaaaaacgt ttaagggtgg
    46561 gtggcatgtt tgttccgttt ctcattatgt tgcgcgaagg acttgaagcc gcgctgattg
    46621 tcagtttgat tgccagctat cttaagcgta cccagcgagg ccgatggatt ggtgtgatgt
    46681 ggattggcgt gttgcttgcc gctgcgttgt gcctgggctt ggggatcttc attaacgaaa
    46741 ccaccggcga atttccgcaa aaagagcagg aactgtttga aggtatcgtg gcggtgatcg
    46801 ccgtggtgat ccttacctgg atggttttct ggatgcgcaa agtgtcgcgc aacgtcaaag
    46861 tgcaactgga acaggcagtc gatagcgcat tgcagcgtgg aaatcatcat ggctgggcgc
    46921 tggtgatgat ggtctttttt gccgttgcaa gggaagggct ggagtcggtc tttttcctgc
    46981 tggcggcatt tcaacaagat gtcgggatct ggccgccgct gggtgcaatg ctcggtcttg
    47041 ctactgccgt ggtgctcggc ttcctgctct actggggcgg tattcgcctc aatcttggtg
    47101 cattttttaa atggaccagc ctgtttattc tcttcgtcgc cgcagggctg gcggctggtg
    47161 ccattcgcgc atttcatgaa gccggattgt ggaaccactt tcaggaaatc gccttcgata
    47221 tgagcgcggt cctctcaact cactcgctgt ttggcacgct gatggaaggg atttttggct
    47281 atcaggaagc accgagcgtc agcgaagtcg ccgtctggtt tatttatctc atcccggcgc
    47341 tggtggcatt tgccctgcca ccacgcgcag gggcgacagc gtctcgctcc gcgtagcaaa
    47401 tacgacgcaa actcttgctt agttacaaca tactttaaag ggatagtctc gtcatgacca
    47461 ttaacttccg ccgtaacgca ttgcagttga gcgtggctgc gctgttctct tctgctttta
    47521 tggctaacgc cgctaatgtg ccgcaggtca aggtgaccgt gaccgataag cagtgcgaac
    47581 cgatgaccat tacggttaac gccgggaaaa cacagttcat tattcagaac cacagccaga
    47641 aggcgctgga gtgggagatc ctcaaaggcg tgatggtggt ggaagagcgg gaaaatatcg
    47701 cccctggctt tagccagaaa atgacggcga atttacagcc tggcgaatac gatatgacct
    47761 gcggtctgct gactaacccg aaagggaagt tgatcgtcaa aggtgaggca acggcggatg
    47821 cggcgcaaag tgatgcgctg ttaagtcttg gtggtgcaat tactgcatat aaagcgtatg
    47881 tcatggcgga aaccacgcag ctggtgaccg acacgaaagc ctttactgac gctattaaag
    47941 caggcgatat cgaaaaagcg aaagcactgt atgcgccgac gcgccagcac tatgagcgca
    48001 tcgaaccgat tgctgaactg ttctccgatc tggatggcag cattgacgcc cgtgaagatg
    48061 attacgagca aaaagccgcc gatccgaaat tcaccggttt ccaccgtctg gaaaaagcat
    48121 tgtttggcga caacaccacc aaaggcatgg atcagtacgc tgaccagctt tataccgatg
    48181 tggtcgattt gcaaaaacgc atcagtgaac tggctttccc accttcaaaa gtggtcggcg
    48241 gtgcagccgg actgattgag gaagtggcag ccagcaaaat cagcggtgaa gaagatcgct
    48301 acagccacac cgatctgtgg gatttccagg ctaacgttga aggctcgcag aaaattgtcg
    48361 atctgctgcg tccacaactg caaaaagcca acccggaact gttggcaaaa gtcgatgcca
    48421 actttaaaaa ggtcgatacc attctggcga aataccgtac taaagacggt tttgaaacct
    48481 acgacaaatt gaccgatgcc gaccggaatg cactgaaagg accgattact gcgctggcgg
    48541 aagatctggc gcaacttcgc ggtgtgctgg gattggatta agcattatgc agtatgaaga
    48601 taaaaacggc gtgaatgaac cgtcacgccg acgtttactg aaaggcattg gtgcgctggc
    48661 gctggcggga agttgtccgg tcgctcatgc acaaaaaacg caaagtgcgc cgggtacgct
    48721 ttcaccggat gcacgcaatg agaaacagcc gttttatggt gagcatcagg cagggatcct
    48781 gacgccacaa caggccgcaa tgatgctggt ggcgtttgat gtgcttgcca gcgataaagc
    48841 cgatcttgag cggttgtttc gcttgttgac tcagcgtttt gcttttctga ctcagggcgg
    48901 agcagcacca gaaacgccaa atccgcgcct gccaccactc gattccggca ttcttggcgg
    48961 ctacattgcg cccgataatc tcaccatcac gttatcggtg ggtcactcat tgtttgatga
    49021 gcgctttggc cttgcgccac agatgccaaa aaagctgcag aagatgacgc gtttccccaa
    49081 cgactcgctg gatgcggcgt tatgtcatgg tgatgtgttg ctacagattt gcgccaacac
    49141 ccaggacacg gtgatccatg cgctgcgcga tatcatcaaa cacacgccgg atttgctcag
    49201 tgtgcgctgg aagcgggaag ggtttatttc cgatcacgcg gcgcgtagta aaggcaaaga
    49261 gacgccgatt aatttgctgg gtttcaaaga cggcactgcc aatcccgata gccagaatga
    49321 taagttgatg caaaaagtgg tgtgggtaac ggcagatcag caggagcctg cgtggacaat
    49381 cggtggcagc tatcaggcag tacgcttgat tcagtttcga gtggaatttt gggacagaac
    49441 gccgctgaaa gaacagcaga cgatttttgg ccgtgataag caaaccggtg cgccgctggg
    49501 aatgcagcat gagcatgatg tgcctgatta cgccagcgac ccggaaggga aggtgatcgc
    49561 gctggacagc catatccggc tggcgaatcc ccgcacggcg gagagtgagt ccagcctgat
    49621 gctgcgtcgc ggctacagtt attcgttggg ggtcaccaac tccggacaac tggatatggg
    49681 gctactgttt gtctgttacc aacacgatct ggaaaaaggc ttcctgacag tacaaaaaag
    49741 gctcaatggc gaagcgctgg aggaatacgt taaacctatc ggcggcggtt atttttttgc
    49801 gctgccgggg gtgaaggacg cgaacgatta tttcggaagc gcgttattgc gggtttaatg
    49861 tttttaggcg gataaggcat ttgtgcgcag atgcctgatg cgacgcttgc gcgtcttatc
    49921 atgcctacaa tcagtgcggg tttggtaggc tggataaggc gttcacgccg catccggcga
    49981 tcgtgcactg atgcctgatg caaatcctgc tgaaagcaca cagctttttt catcactgtc
    50041 atcactctgt catctttcca gtagaaacta atgtcactga aatggtgttt tatagttaaa
    50101 tataagtaaa tatattgttg caataaatgc gagatctgtt gtacttatta agtagcagcg
    50161 gaagttcccg gcagtgatag tcagtcacta tggagatcgc ggatggtaac gtcctgtact
    50221 ggacatgttt tagataatca acgcgccacc actcgcggag ttttctcctc cggtagtcat
    50281 ctcgtcactt tgcattttca acctcatcct ttcttttcat gtgttaccga cgccgtaaat
    50341 ggtgcgcgta gccgtttttc ggcattttat ccaaaagcaa actatggctt acaaggaagc
    50401 caaccctctg atgttcgtgc gcataatcgc gctgccaacg gcgcgtgtga tgaatacaaa
    50461 caactcaagg tgctctccat gggaagacaa aaagcagtga tcaaagctcg tcgcgaggca
    50521 aaacgtgtgc tgagacggga ttcgcgcagc cataagcagc gtgaagaaga atcggtcacc
    50581 tcgcttgtgc agatgggcgg cgtagaagcc attggtatgg cccgcgacag tcgcgatact
    50641 tcgcccatcc tcgcgcgaaa tgaagcgcaa ttgcactatc tgaaggctat tgagagtaag
    50701 cagctgatat tcgccacggg cgaagccggg tgcggaaaaa cctggatcag cgcagcaaaa
    50761 gcggcagagg ccctgataca taaggatgtc gacaggatta tcgtcacccg tccagttctg
    50821 caagccgatg aagatcttgg cttcttacct ggagatatcg cagaaaagtt tgctccctat
    50881 tttcgcccgg tctacgacgt gctggtccgg cgcttagggg cttcctttat gcagtactgc
    50941 ctgcgaccgg aaatagggaa ggtggaaatt gcgccgttcg cctatatgcg tggacgtacc
    51001 tttgaaaatg cagtcgtcat tcttgacgag gcgcagaatg tgactgccgc gcaaatgaaa
    51061 atgtttttaa cccgcctcgg ggagaacgtg acggttatcg tcaacggtga tatcacgcaa
    51121 tgcgatttgc ctcgcggcgt gtgctccgga ttaagtgacg cgctggaacg tttcgaagaa
    51181 gatgaaatgg ttgggatcgt ccggttcggt aaagaggact gcgtacgttc ggcactttgc
    51241 caacgtacgc tgcatgccta cagttaagtg tgttatcggt gcagagcccg ggcgaaccgg
    51301 gctttgtttt atttcaacag attttcactt caggttagcg ttcagggggg caactttgag
    51361 cgtgttgata agctgaactg gagtctggtt tagatactct taccaggcat ttttcgtcat
    51421 agcatcagac cagtgtaata ctgccagcgc ggagagtttt tcttccagaa gccaaaaaaa
    51481 tgagcatctg cctatctttt ttcggccatt caacctgtac aggagatctg gtgccaattt
    51541 tgcttatgag gtttaatcaa caatgggcca taatttgcct gactgattaa cactgaatat
    51601 attcct
//
LOCUS       DAZFZC010000022        50952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFZC010000022 DAZFZC010000000
VERSION     DAZFZC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.022"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..916
                     /gene="ydiY"
                     /locus_tag="ACHKXP_001535"
     CDS             158..916
                     /gene="ydiY"
                     /locus_tag="ACHKXP_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO7646570.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(969..1076)
                     /gene="yniD"
                     /locus_tag="ACHKXP_001536"
     CDS             complement(969..1076)
                     /gene="yniD"
                     /locus_tag="ACHKXP_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO7646571.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1249..3147)
                     /gene="espL1"
                     /locus_tag="ACHKXP_001537"
     CDS             complement(1249..3147)
                     /gene="espL1"
                     /locus_tag="ACHKXP_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFO7646572.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTAQGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIKII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGEPGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPALYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            3670..5598
                     /gene="thrS"
                     /locus_tag="ACHKXP_001538"
     CDS             3670..5598
                     /gene="thrS"
                     /locus_tag="ACHKXP_001538"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO7646573.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            5602..6144
                     /gene="infC"
                     /locus_tag="ACHKXP_001539"
     CDS             5602..6144
                     /gene="infC"
                     /locus_tag="ACHKXP_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO7646574.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            6241..6438
                     /gene="rpmI"
                     /locus_tag="ACHKXP_001540"
     CDS             6241..6438
                     /gene="rpmI"
                     /locus_tag="ACHKXP_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO7646575.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            6491..6847
                     /gene="rplT"
                     /locus_tag="ACHKXP_001541"
     CDS             6491..6847
                     /gene="rplT"
                     /locus_tag="ACHKXP_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO7646576.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6964..7088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6970..7014
                     /gene="pheM"
                     /locus_tag="ACHKXP_001542"
     CDS             6970..7014
                     /gene="pheM"
                     /locus_tag="ACHKXP_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO7646577.1"
                     /translation="MNAAIFRFFFYFST"
     gene            7298..8281
                     /gene="pheS"
                     /locus_tag="ACHKXP_001543"
     CDS             7298..8281
                     /gene="pheS"
                     /locus_tag="ACHKXP_001543"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO7646578.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            8296..10683
                     /gene="pheT"
                     /locus_tag="ACHKXP_001544"
     CDS             8296..10683
                     /gene="pheT"
                     /locus_tag="ACHKXP_001544"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO7646579.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQISMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            10688..10987
                     /gene="ihfA"
                     /locus_tag="ACHKXP_001545"
     CDS             10688..10987
                     /gene="ihfA"
                     /locus_tag="ACHKXP_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO7646580.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            11088..12068
                     /gene="btuC"
                     /locus_tag="ACHKXP_001546"
     CDS             11088..12068
                     /gene="btuC"
                     /locus_tag="ACHKXP_001546"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO7646581.1"
                     /translation="MLTLDRQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            12131..12682
                     /gene="btuE"
                     /locus_tag="ACHKXP_001547"
     CDS             12131..12682
                     /gene="btuE"
                     /locus_tag="ACHKXP_001547"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO7646582.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWVDRGFMVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            12682..13431
                     /gene="btuD"
                     /locus_tag="ACHKXP_001548"
     CDS             12682..13431
                     /gene="btuD"
                     /locus_tag="ACHKXP_001548"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO7646583.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            13509..13973
                     /gene="nlpC"
                     /locus_tag="ACHKXP_001549"
     CDS             13509..13973
                     /gene="nlpC"
                     /locus_tag="ACHKXP_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO7646584.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            14220..14933
                     /gene="ydiV"
                     /locus_tag="ACHKXP_001550"
     CDS             14220..14933
                     /gene="ydiV"
                     /locus_tag="ACHKXP_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO7646585.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            14996..16432
                     /gene="selO"
                     /locus_tag="ACHKXP_001551"
     CDS             14996..16432
                     /gene="selO"
                     /locus_tag="ACHKXP_001551"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO7646586.1"
                     /translation="MTLSFITRWRDELPATYTTLSPTPLNNARLIWHNAELANTLGIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSLVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(16436..16627)
                     /gene="hemP"
                     /locus_tag="ACHKXP_001552"
     CDS             complement(16436..16627)
                     /gene="hemP"
                     /locus_tag="ACHKXP_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO7646587.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(16759..17805)
                     /gene="aroH"
                     /locus_tag="ACHKXP_001553"
     CDS             complement(16759..17805)
                     /gene="aroH"
                     /locus_tag="ACHKXP_001553"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO7646588.1"
                     /translation="MNRTDELRTAHIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17962..18795)
                     /gene="ppsR"
                     /locus_tag="ACHKXP_001554"
     CDS             complement(17962..18795)
                     /gene="ppsR"
                     /locus_tag="ACHKXP_001554"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO7646589.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            19128..21506
                     /gene="ppsA"
                     /locus_tag="ACHKXP_001555"
     CDS             19128..21506
                     /gene="ppsA"
                     /locus_tag="ACHKXP_001555"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO7646590.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(21563..23209)
                     /gene="fadK"
                     /locus_tag="ACHKXP_001556"
     CDS             complement(21563..23209)
                     /gene="fadK"
                     /locus_tag="ACHKXP_001556"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO7646591.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMSDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTTSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(23266..23559)
                     /gene="ydiT"
                     /locus_tag="ACHKXP_001557"
     CDS             complement(23266..23559)
                     /gene="ydiT"
                     /locus_tag="ACHKXP_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7646592.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIIMAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(23556..24845)
                     /gene="ydiS"
                     /locus_tag="ACHKXP_001558"
     CDS             complement(23556..24845)
                     /gene="ydiS"
                     /locus_tag="ACHKXP_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7646593.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVIIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERTDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(24901..25839)
                     /gene="ydiR"
                     /locus_tag="ACHKXP_001559"
     CDS             complement(24901..25839)
                     /gene="ydiR"
                     /locus_tag="ACHKXP_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO7646594.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDAAAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCASDNSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(25859..26623)
                     /gene="ydiQ"
                     /locus_tag="ACHKXP_001560"
     CDS             complement(25859..26623)
                     /gene="ydiQ"
                     /locus_tag="ACHKXP_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO7646595.1"
                     /translation="MKIITCFKLVPEEQGIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            26939..27850
                     /gene="ydiP"
                     /locus_tag="ACHKXP_001561"
     CDS             26939..27850
                     /gene="ydiP"
                     /locus_tag="ACHKXP_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7646596.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWLLTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(27893..29044)
                     /gene="ydiO"
                     /locus_tag="ACHKXP_001562"
     CDS             complement(27893..29044)
                     /gene="ydiO"
                     /locus_tag="ACHKXP_001562"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO7646597.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(29058..30653)
                     /gene="ydiF"
                     /locus_tag="ACHKXP_001563"
     CDS             complement(29058..30653)
                     /gene="ydiF"
                     /locus_tag="ACHKXP_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7646598.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVARDEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(30796..31554)
                     /gene="aroD"
                     /locus_tag="ACHKXP_001564"
     CDS             complement(30796..31554)
                     /gene="aroD"
                     /locus_tag="ACHKXP_001564"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO7646599.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(31585..32451)
                     /gene="ydiB"
                     /locus_tag="ACHKXP_001565"
     CDS             complement(31585..32451)
                     /gene="ydiB"
                     /locus_tag="ACHKXP_001565"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO7646600.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNQALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(32463..33728)
                     /gene="ydiN"
                     /locus_tag="ACHKXP_001566"
     CDS             complement(32463..33728)
                     /gene="ydiN"
                     /locus_tag="ACHKXP_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7646601.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFCTRLNTIKHRG"
     gene            complement(33955..35169)
                     /gene="ydiM"
                     /locus_tag="ACHKXP_001567"
     CDS             complement(33955..35169)
                     /gene="ydiM"
                     /locus_tag="ACHKXP_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7646602.1"
                     /translation="MKNPYFPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYGLG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFAVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKETW
                     HHSLKENVAPGG"
     gene            complement(35269..35625)
                     /gene="ydiL"
                     /locus_tag="ACHKXP_001568"
     CDS             complement(35269..35625)
                     /gene="ydiL"
                     /locus_tag="ACHKXP_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO7646603.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(35761..35868)
                     /gene="rprA"
                     /locus_tag="ACHKXP_001569"
     ncRNA           complement(35761..35868)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKXP_001569"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(36054..37166)
                     /gene="ydiK"
                     /locus_tag="ACHKXP_001570"
     CDS             complement(36054..37166)
                     /gene="ydiK"
                     /locus_tag="ACHKXP_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO7646604.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            37555..40611
                     /gene="ydiJ"
                     /locus_tag="ACHKXP_001571"
     CDS             37555..40611
                     /gene="ydiJ"
                     /locus_tag="ACHKXP_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO7646605.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            40608..41018
                     /gene="menI"
                     /locus_tag="ACHKXP_001572"
     CDS             40608..41018
                     /gene="menI"
                     /locus_tag="ACHKXP_001572"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO7646606.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            41118..41306
                     /gene="ydiH"
                     /locus_tag="ACHKXP_001573"
     CDS             41118..41306
                     /gene="ydiH"
                     /locus_tag="ACHKXP_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO7646607.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            41854..42222
                     /gene="sufA"
                     /locus_tag="ACHKXP_001574"
     CDS             41854..42222
                     /gene="sufA"
                     /locus_tag="ACHKXP_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO7646608.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            42231..43718
                     /gene="sufB"
                     /locus_tag="ACHKXP_001575"
     CDS             42231..43718
                     /gene="sufB"
                     /locus_tag="ACHKXP_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO7646609.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            43728..44474
                     /gene="sufC"
                     /locus_tag="ACHKXP_001576"
     CDS             43728..44474
                     /gene="sufC"
                     /locus_tag="ACHKXP_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO7646610.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVHPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            44449..45720
                     /gene="sufD"
                     /locus_tag="ACHKXP_001577"
     CDS             44449..45720
                     /gene="sufD"
                     /locus_tag="ACHKXP_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO7646611.1"
                     /translation="MAGLPNSSNALQQWHHLFEADGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDSVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            45717..46937
                     /gene="sufS"
                     /locus_tag="ACHKXP_001578"
     CDS             45717..46937
                     /gene="sufS"
                     /locus_tag="ACHKXP_001578"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO7646612.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPGQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            46950..47366
                     /gene="sufE"
                     /locus_tag="ACHKXP_001579"
     CDS             46950..47366
                     /gene="sufE"
                     /locus_tag="ACHKXP_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO7646613.1"
                     /translation="MAVLPDKEKLLRNFLRCTNWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            47515..48519
                     /gene="ldtE"
                     /locus_tag="ACHKXP_001580"
     CDS             47515..48519
                     /gene="ldtE"
                     /locus_tag="ACHKXP_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO7646614.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(48583..48819)
                     /gene="lpp"
                     /locus_tag="ACHKXP_001581"
     CDS             complement(48583..48819)
                     /gene="lpp"
                     /locus_tag="ACHKXP_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO7646615.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(49130..50542)
                     /gene="pykF"
                     /locus_tag="ACHKXP_001582"
     CDS             complement(49130..50542)
                     /gene="pykF"
                     /locus_tag="ACHKXP_001582"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO7646616.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(50550..50744)
                     /locus_tag="ACHKXP_001583"
     CDS             complement(50550..50744)
                     /locus_tag="ACHKXP_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646617.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT        12960 a        12206 c        13041 g        12745 t
ORIGIN      
        1 gccacatgct tattcaaaaa atgaaataag aatacattgc taagttattc gcgccttttt
       61 ataattcgtt acctttccag ggacgctaat ctttgatcct taagaaagtt tccgggaccg
      121 cgatggtctc tttttttgtt gtacggactc ttgataaatg aagcttttga agacagttcc
      181 tgccatcgtt atgttggcgg ggggcatgtt tgcgtcactg aatgcagctg ccgatgattc
      241 cgtttttact gtcatggacg atcctgccag cgctaaaaaa cctttcgaag gtaatctgaa
      301 cgcaggatat cttgcacaat caggcaatac gaaaagctcc tcacttacgg ctgacaccac
      361 catgacctgg tatggacaaa ccactgcctg gtcgctgtgg gggaacgcca gtaatacttc
      421 ttctaatgat gagcgttctt cagaaaaata tgcggcgggc gggcgtagcc gttttaattt
      481 aactgattat gactatttat ttggtcaggc aagttggctg acagaccgtt ataacggcta
      541 tcgagagcgc gatgtgttga ccgcgggtta tggtcgccag tttttgaatg gtccggtaca
      601 cagtttccgc tttgaattcg gcccaggtgt gcgttacgac aaatatactg acaatgccag
      661 tgaaacccag ccgctgggct atgcctccgg ggcttatgca tggcagttga ctgacaacgc
      721 taaatttacg cagggtgttt cggtctttgg cgcggaagac acaacgctga actccgaaag
      781 cgcattaaat gttgctatta atgaacattt tggactgaag gtggcttaca acgttacatg
      841 gaactctgaa cctccagagt ctgcaccgga acataccgac cgtcggacaa ccttatcgct
      901 gggctacagc atgtaaaatt atctgccgga ccgtgttatg cggtctggct gtcttcgttt
      961 gtcactgctc agccccaaat cattgctgcc caacgtagca acaacatagc gccacagatt
     1021 gccagtagca ccaccaccat cttccagtgt tttttatcaa agcgtttagt cggcatacca
     1081 gtgtcctgca tatctcttgt gcgtgataac ggagtgtaac aaaagggacg ggcatcaagg
     1141 gattttttgt tattacagcc gttgggtggc gttgtacacc tggtcactgt ttctgcgttt
     1201 tatgaaaaaa taatattatg catcaacata tatatcgaaa atagttggtt ataaatacgt
     1261 ctttagttca tcagcactaa aactcaggct ttggctgatt acatttatag cagcataata
     1321 tgtttctaca gttttataat gattatgatg aatggctata tgaacagctg acatgttgtc
     1381 atgattttta gcggccaaca gtgtgaataa ctgacgttga ctaaaagaat atttttttgc
     1441 aaaaatattc agcgtcgata tataagataa cacgacgtct tcattaccct tgctcatggc
     1501 gatgtataat gcaggggttc catgtgctgt agcgcctttt aataaatcca tgatgttagc
     1561 tttgctcaac ttgtatttaa aggcgatgcc attaatttta gagaggaacc gtgtgacaca
     1621 caaagggtga ttattttcca ttgcagcaaa caaccctggt tcaccatcac gtgttttcat
     1681 tgctaaaata tccatcacta tttttttgtt taaaagctct tgtgctattg gagtggttaa
     1741 tgcattcaga aagatatcga ttgtttcatc gaacccatac aacatggaga tatagagccc
     1801 aggtgtttcg ttagcacttt tagtttgcag taatttaacc agattatctt catgaatgat
     1861 atgattgtat atcagtgact gaatttcttg cataaatatt tttacgttgt cagtatgtcc
     1921 gttcatcata gccagaaata aagcaggtgt accaacgcta ttctgcgcag ttattatttt
     1981 aatgatttct ttttcaggga gtatgggcaa ctgtttgaat aatgtaataa gctgttgagt
     2041 ctggcctgtg gccattatta acaacattgc cgatggatgt ataggggcaa atggcattcg
     2101 tgatgcaata cccgcgaaaa tagcccctgg aactcccaca ggtaataggg gcagaatagc
     2161 aattgcacag ttgcgaacat acctatcagc ccaagtctga gcatcgggac ctgaactgat
     2221 gaacttatca agcgagagaa agccctgtgc tgtcctgtgg gtgcctctga cggcaacgtt
     2281 ggtatcgttc gggtcgtaaa cagacacaac ataatttaat tgtccatacg ctgactgctt
     2341 tattcgtagc cttactgaca aagtatgatt gtctatatat aaaatcgcgg acatatgact
     2401 gacattgttt gccatcattc gacaaaagaa aatatctaaa gcgaagccaa aatccttact
     2461 cgcgataacg cgactccctg caggaggatg gcgaagtaat gctctgaact ccgcgtcccg
     2521 gactaagggg acatgctgct gtatggattc aacggaggag tagtgtttaa tcaggttgac
     2581 tttgcctttt ccatttctca atgaatcaag ggcgtattgc aatgacagat ggcgacaaaa
     2641 aatagcgtca gaaggagatt gcgaaaaaaa tgcactacca ttgagattta ctgttggttg
     2701 tatagttttt tctctatgga tttttttaga cagtttcata caatggttac gatcttcggc
     2761 agttaactgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ccttaatagg
     2821 cgtggtttcc agaaataagg gaatgatttc ttttttatag tgtaatgtaa tgagtatatt
     2881 ttctgcatgg atgggtgaag tggcaaagtt aatgataaaa agggcagtgg aataacaatt
     2941 ttgtgatatt tttcgacagt ttgcggtaaa ttcctgagta acacagtatg acagcatcga
     3001 acttaagtca gaggtatcaa gtagggtgat gagttggcgc gcaaattgat taataggctc
     3061 tgatgaaggc gttggtatgt cgctatcgat gtgcaatcta tgaggagact tataatgagt
     3121 tctgataatg atatcgtttt gactcataat ctattcgcct tatatatgtc tggagatttt
     3181 gaggatgatg attaattatt atcgccagaa cgaaatgtgc acgaaaaatt gctataagaa
     3241 ttgtgtcact tttatcattg tgatatgacc agttcgtcat taatctcatg atcgtttaaa
     3301 tactaccgcc aaatcatgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
     3361 ctcgcagtaa acctttcaga cgctccgtga tgttatcagt tgttctttaa gcgttttgct
     3421 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
     3481 cccttttttt agtgttaaca tcgctcaacc gggtggaagg tgagaggtta atgtctttca
     3541 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
     3601 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
     3661 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgatcacgct
     3721 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     3781 gggcgcgtta atggcgaact agttgatgct tgcgatctga ttgaaaacga cgcacaactg
     3841 tcgatcatta ccgcgaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     3901 ctgttagggc acgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     3961 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     4021 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     4081 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtc
     4141 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     4201 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     4261 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     4321 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     4381 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacaac tcgacctgta tcatatgcag
     4441 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatttt ccgtgaactg
     4501 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     4561 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     4621 ttcaccacgt cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     4681 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     4741 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     4801 ggttttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     4861 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     4921 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     4981 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     5041 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     5101 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     5161 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tcttgggtcg
     5221 atggaacgtt tcatcggtat cctgaccgag gaattcgctg gtttcttccc gacctggctt
     5281 gcgccggttc aggttgttat catgaatatt accgattcac agtctgatta cgttaacgaa
     5341 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     5401 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     5461 gataaagagg tggaatctgg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     5521 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     5581 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     5641 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     5701 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     5761 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     5821 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     5881 aaggaaatta aattccgtcc tggtacagat gaaggcgact atcaggtaaa actccgcagc
     5941 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     6001 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     6061 gaactggcag tggtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     6121 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tgtctgtgga gccttcgggc
     6181 ttcacaggct ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     6241 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     6301 ggttttaagc acaagcacgc taacctgcgt cacattctga ccaaaaaagc gaccaaacgt
     6361 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     6421 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     6481 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     6541 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     6601 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     6661 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     6721 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     6781 tatcgcagta ttcgacaaag tagcgttcac cgctctggtt gaaaaagcga aagcagctct
     6841 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     6901 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     6961 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     7021 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     7081 ttttttgtat gcgcgttttg aaaaataaaa agcctgtctc atcaggtgta attgtcgcag
     7141 ccagactgga cacggccagc gcggagaaac cggagcgtat ttaagtacgt gagaatttcg
     7201 agcacagccc gggaccaaaa tggcaagtaa aatagcctga tgggataggc tctaagtcca
     7261 acgaaccagt gtcaccactg acacaatgag gaaaaccatg tcacatctcg cagaactggt
     7321 tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg
     7381 cgtcgaatat ttgggtaaaa aagggcactt aacccttcag atgacgaccc tgcgtgagct
     7441 gccgccagaa gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag agcaggttca
     7501 gcaggcgctg aatgcgcgta aagcggaact ggaaagcgct gcactgaatg cgcgtctggc
     7561 ggcggaaacg attgatgtct ctctgccagg tcgtcgcatt gaaaacggcg gtctgcatcc
     7621 ggttacccgt accatcgacc gtatcgaaag tttcttcggt gagcttggct ttaccgtggc
     7681 aaccgggccg gaaatcgaag acgattatca taacttcgat gctctgaaca ttcctggtca
     7741 ccacccggcg cgcgctgacc acgacacttt ctggtttgac actacccgcc tgctgcgtac
     7801 ccagacctct ggcgtacaga tccgcaccat gaaagcccag cagccaccga ttcgtatcat
     7861 cgcgcctggc cgtgtttatc gtaacgacta cgaccagact cacacgccga tgttccatca
     7921 gatggaaggt ctgattgttg ataccaacat cagctttacc aacctgaaag gcacgctgca
     7981 cgacttcctg cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc cttcctactt
     8041 cccgtttacc gaaccttctg cagaagtgga cgtcatgggt aaaaacggta aatggctgga
     8101 agtgctgggc tgcgggatgg tgcatccgaa cgtgttgcgt aacgttggca tcgacccgga
     8161 agtttactct ggtttcgcct tcgggatggg gatggagcgt ctgactatgt tgcgttacgg
     8221 cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac agtttaaata
     8281 aggcaggaat agattatgaa attcagtgaa ctgtggttac gcgaatgggt gaacccggcg
     8341 attgatagcg atgcgctggc aaatcaaatc tctatggcgg gcctggaagt tgacggtgta
     8401 gaaccggttg ccggcagctt ccacggcgtg gtcgttggtg aagtggttga gtgtgcgcag
     8461 catccgaacg ctgacaaact gcgtgtgaca aaagtgaatg tcggcggcga tcgcctgctg
     8521 gacatcgtct gcggtgcgcc aaactgccgt cagggcctgc gtgtggcggt agcgaccatt
     8581 ggtgctgttc tgccgggtga tttcaaaatt aaagcggcga aactgcgtgg cgaaccgtct
     8641 gaagggatgc tgtgctcctt ctctgaactg ggcatttctg acgatcacag cggcattatc
     8701 gaactgcctg cggatgcgcc gattggcacc gatatccgtg aatacctgaa acttgatgac
     8761 aacaccatcg aaatcagcgt gacgccaaac cgtgccgact gcttaggcat cattggtgtt
     8821 gcgcgtgacg ttgccgtgct gaaccagctg ccgctggttc aaccggaaat cgttccggtt
     8881 ggtgcgacca tcgacgacac gctgccgatt acagtcgaag cgccggaagc ctgcccgcgt
     8941 tatcttggcc gtgtggtaaa aggcattaac gttaaagcgc caactccgct gtggatgaaa
     9001 gaaaaactgc gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt caccaactat
     9061 gtgctgctcg aactgggcca gccgatgcac gctttcgata aagatcgcat tgaaggcggc
     9121 attgtggtgc ggatggcgaa agagggcgaa acgctggtgc tgctcgacgg tactgaagcg
     9181 aagctgaatg ctgacactct ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc
     9241 atcttcggtg gcgaacactc tggcgtgaat gacgaaacac aaaacgtgct gctggaatgc
     9301 gcgttcttta gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct gcataccgat
     9361 gcgtctcacc gttatgagcg tggcgttgat ccggcactgc agcacaaagc gatggaacgt
     9421 gcgacccgtc tgctgatcga catctgcggt ggtgaggctg gcccggtaat tgatatcacc
     9481 aacgaagcaa cgctgccgaa gcgtgcaacc atcactctac gtcgtagcaa actggatcgc
     9541 ctgatcggcc atcatattgc ggatgagcag gtaactgaca ttctgcgtcg tctcggctgc
     9601 gaagtgaccg aaggcaaaga cgaatggcag gcagttgcgc cgagctggcg tttcgatatg
     9661 gagattgaag aagatctggt tgaagaagtc gcgcgtgttt acggctacaa caacatcccg
     9721 gatgagccgg tacaggcaag cctgattatg ggtactcacc gtgaagctga cctgtcgctc
     9781 aagcgcgtga aaacgctgct caacgacaaa ggctatcagg aagtgatcac ctacagcttc
     9841 gttgatccga aagtgcagca gatgatccat ccaggcgttg aagccttact gctgccaagc
     9901 ccgatctctg ttgaaatgtc agcaatgcgt ctttctctgt ggactggcct gctggcaacc
     9961 gtggtgtaca accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag cggtctgcgt
    10021 ttcgtaccag atactcaggc accgttgggc attcgtcagg atctgatgtt agccggtgtg
    10081 atttgcggta accgttacga agagcactgg aacctggcaa aagagaccgt tgatttctat
    10141 gatttgaaag gcgatcttga atccgttctc gacctgaccg gtaaactgaa tgaggttgag
    10201 ttccgtgcag aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa
    10261 ggtgaacgta ttggttttgt tggggttgtt catcctgaac tggaacgtaa actggatctt
    10321 aacggtcgca ctctggtgtt cgaactggag tggaacaagc tcgcagaccg cgtggtgcct
    10381 caggcgcgcg agatttctcg cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca
    10441 gaaaacgttc ccgcagcgga tattttatcc gaatgtaaga aagttggcgt aaatcaggta
    10501 gttggcgtaa acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc
    10561 ctcgccataa gcctgatcct gcaagatacc agccgtacac tcgaagaaga ggagattgcc
    10621 gctaccgtcg ccaaatgtgt agaggcatta aaagagcgat tccaggcatc attgagggat
    10681 tgaacctatg gcgcttacaa aagctgaaat gtcagaatat ctgtttgata agcttgggct
    10741 tagcaagcgg gatgccaaag aactggttga actgtttttc gaagagatcc gtcgcgctct
    10801 ggaaaacggc gaacaggtga aactctctgg ttttggtaac ttcgatctgc gtgataagaa
    10861 tcaacgcccg ggacgtaacc cgaaaacggg cgaggatatt cccattacag cacggcgcgt
    10921 ggtgaccttc agacccgggc agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga
    10981 cgagtaatct gatctaacta aaaaggccgc tctgcggcct tttttctttt cactgtcgaa
    11041 gagtcaccgt aaaatcaacg ccatgacact tcagcagaac ggataccatg ctgacacttg
    11101 accgccaaca acagcgacaa aatattcgct ggttattatg cctgtcagtt ttgatgctgc
    11161 tggcgcttct cttaagcctt tgcgccggtg aacaatggat ctcgccaggt gactggttta
    11221 ctcctcgtgg cgaactgttc gtctggcaaa ttcgcctgcc acgtacgctg gctgtattgc
    11281 tggttggtgc ggcgctggct atatccggcg ctgtaatgca ggcgttgttt gaaaatcctc
    11341 tggcagaacc tggactactt ggcgtctcta acggcgcagg cgtggggctt atcgccgcgg
    11401 tattgcttgg gcaagggcaa ctccccaact gggcgctagg gctgtgtgcg attgctggcg
    11461 cgcttatcat cactttaata ctcttacgtt tcgcccgtcg tcatctttcg accagtcggt
    11521 tattgctggc tggcgttgca ttagggatta tctgtagcgc actaatgacg tgggctatct
    11581 acttttccac ctctgttgat ttacgtcagc tgatgtactg gatgatgggc ggttttggcg
    11641 gcgtagactg gcggcaaagc tggctgatgc tggcattgat ccccgtgttg ttgtggatct
    11701 gttgtcagtc caggccgatg aatatgttag cacttggcga gatctcggcg cggcaactgg
    11761 gtttacccct gtggttctgg cgcaatgtgc tggtggccgc gaccggctgg atggttggcg
    11821 tcagtgtggc gctggcgggt gctatcggct ttattggtct ggtgatccca catattcttc
    11881 ggttgtgtgg tttaaccgat catcgcgtat tacttcccgg ctgcgcgctg gcaggggcga
    11941 gcgcattgct gctggccgat attgtagcgc gcctggcatt agctgccgca gagctgccta
    12001 ttggcgtggt caccgcaacg ttgggtgcgc cggtgtttat ctggttattg ttaaaagcaa
    12061 gacgttagcc gcaaaaagac ggtctatgat taaaagctaa gatttttact gaccacaccc
    12121 aggagaaacg atgcaagatt ccattctgac gaccgtagtg aaagatatcg acggtgaagt
    12181 gaccacgctg gagaagttcg ccggtaatgt gctgttgatt gtcaatgtcg cctcaaagtg
    12241 tggcttaacg ccgcaatatg agcagttgga gaatattcag aaagcctggg tcgatcgagg
    12301 ttttatggtg ctgggattcc cgtgcaacca gtttctggaa caagaaccgg gcagcgatga
    12361 agagattaaa acttactgta ccaccacatg gggggtgacg ttcccgatgt tcagtaagat
    12421 tgaagttaat ggcgaaggac gccatccgct gtatcaaaaa ttgattgccg cagcgccgac
    12481 cgcagtcgcg ccggaagaga gcggattcta tgcccgtatg gtcagcaaag gccgtgcacc
    12541 gctgtacccg gatgatattt tatggaattt tgaaaaattc ctggttggca gggacggaaa
    12601 agtcatccag cgtttttccc cggatatgac gccggaagat cccattgtga tggaaagcat
    12661 taaactggcg ttggcaaaat aatgtctatt gtgatgcagt tacaagatgt tgcggaatct
    12721 acccgcctgg ggccgctttc tggcgaggtt cgggctgggg agatcctgca cctggtgggg
    12781 ccgaatggcg cgggtaagag taccttactg gcgcgaatgg ccggaatgac cagcggtaag
    12841 ggaagcattc agttcgcggg gcaaccactg gaagcatggt ccgcaacaaa actcgcgctg
    12901 catcgcgcct atctttcaca acagcagacg ccgccgtttg caatgccggt ctggcactac
    12961 ctgacactgc atcagcacga taaaacgcgt accgaactac tgaatgatgt cgcaggggcg
    13021 ctggctcttg atgacaaact cggacgtagc accaatcaac tttccggcgg tgaatggcaa
    13081 cgcgtacgtc ttgctgcggt ggtgttgcaa atcacaccac aagccaatcc cgcaggccaa
    13141 ttgctgcttc ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag tgcgttagac
    13201 aaaattctga gcgcgctgtg tcagcaagga ctggcgattg tgatgagcag tcacgatctc
    13261 aaccacacat tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc
    13321 agtggacgca gggaagaggt gctcacgccg ccaaatctgg cgcaggccta tgggatgaat
    13381 tttcgccgtc tggatatcga aggtcacaga atgctgattt cgaccatctg atttcattag
    13441 tatcttgaaa atcgggtaat tacgacgcta aattaatgca agaataaaaa acagaggaac
    13501 cgtacggaat gcgtttctgc cttattttga tcacagcact gcttctggcc gggtgtagcc
    13561 accataaagc accgccgcca aatgccagac tttctgattc gattaccgtt attgccggtt
    13621 tgaacgacca gctacaaagc tggcatggca cgccgtatcg ttatggtggc atgacgcggc
    13681 gcggtgtgga ctgttcggga tttgtggttg tgacgatgcg cgatcgtttc gatttgcagc
    13741 tgccccgtga aaccaaacaa caagcctcta ttggcacgca aattgataaa gacgagttgc
    13801 tgccaggtga cctggtcttt ttcaaaacgg ggtccggaca aaatggtttg catgtaggta
    13861 tttacgatac caacaaccaa tttatccacg cctctaccag caagggagtg atgcgttcct
    13921 cacttgataa tgtctattgg cagaaaaatt tctggcaggc gagacgaatc tagtatgtcg
    13981 aaaaatggac ggcgatgata catcgccgtc catttactcc tgaatacagt agtattttgc
    14041 agacaaaaat tccgtaaatg gattattcgt ttataatcag gataatctaa aaaaaggacc
    14101 cctgaaaaag tggttaacat ggaacaaatg aaagttactc cttctgtaat tgttatgtct
    14161 tatggcctca tcccttttag ccggatactg aaaaacatcc ttcgagaggg acggttacca
    14221 tgaagatttt tttggagaat ctttatcatt ctgattgcta tttcctgccg ataagagaca
    14281 accagcaagt ccttgtcggc gttgagttaa ttacccattt ctccagtgaa gatggcacgg
    14341 tgcgtatccc gacgagtcgg gttatagcgc aacttaccga ggagcaacat tggcaattat
    14401 tttcagagca gttggaatta ctaaaatcat gccaacattt ttttattcaa cataaactct
    14461 ttgcctggct taatttaacg ccacaagtgg ctacattatt attagagagg gataattacg
    14521 caggcgaact attaaaatat ccgtttattg aactgttgat taatgagaat tacccgcatc
    14581 tcaatgaagg aaaagataat cgcggtcttt tatccttatc gcaagtgtat ccccttgtgt
    14641 taggtaattt gggagcaggc aatagcacaa tgaaagcggt ttttgatggt cttttcaccc
    14701 gcgtcatgct ggataaaagt tttattcagc aacaaatcac gcaccgctcc ttcgaaccgt
    14761 ttattcgtgc gatccaggcg caaatttccc cttgttgcaa ttgcatcatt gcgggaggca
    14821 ttgatacggc agaaatattg gcacagatta cccccttcga tttccatgct ctccagggat
    14881 gcctgtggcc tgccgttcct gtaaatcaga taacgacgtt ggttcagcga taaccctcct
    14941 gtttgctggt gtttaagacg agagtaaccg tctacactat caaacaggag gatctatgac
    15001 cctgtctttt attacccgtt ggcgcgatga attgccagca acctatacaa cactttcccc
    15061 tacgccttta aataatgccc ggctgatttg gcataatgcc gaactggcta acacgctggg
    15121 tattccatcg tcactgttta aaaatggcgc aggtgtctgg ggtggcgaaa ccctactgcc
    15181 tggtatgtca ccactggcgc aggtttacag tggtcatcag ttcggcgtct gggcgggcca
    15241 actgggtgat gggcgcggca ttttactcgg cgaacaacgg cttgctgatg gcactacaat
    15301 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    15361 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    15421 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ctagtgtatc gggaaacggc
    15481 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    15541 acatttttac taccgccgcg agtcggaaaa ggttcgtcag ttagctgact ttgctattcg
    15601 tcattactgg tcacatcttg cagatgatga ggacaaatac cgtctctggt ttagcgatgt
    15661 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg ctcatggggt
    15721 gatgaatacc gacaacatgt cgctgctggg gctgacgctt gattacgggc cgtttggttt
    15781 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttacag
    15841 ctttgataat caacctgccg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    15901 gccatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    15961 gacgcattat ggagaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    16021 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    16081 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgatga
    16141 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    16201 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc ccgctctggt
    16261 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgacgga
    16321 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    16381 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    16441 tggtcaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    16501 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggaaattcgc cgaataggct
    16561 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    16621 aacgcataaa aattaataat aattgatata acaatgattc ttattatcaa tattatggct
    16681 gaccagagca agcagtaatt tgttgagaag acaacaagtt gcgcagaaat gcccattccg
    16741 tcaggaatgg gcacgcattc agaagcgggt atctaccgca gaggcgagtt tttcgaccag
    16801 gcgttcggta tcctcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    16861 ctgactgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    16921 aatcgccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    16981 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    17041 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    17101 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    17161 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    17221 agcaatccgc gtattgccat cggtaccatt tttaaaacct accggacagg agagtgccga
    17281 agccatttcg cggtggatct gactttcggt agtacgtgcg ccaatcgcgc cccaactgat
    17341 taaatcagca ataaactgac cggtcaccat atcgaggaac tcggtcgcgg ttgggacgcc
    17401 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    17461 gctgccgttt aaatctggat cggagattag tcctttccag ccgacaacag ttcgtggttt
    17521 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    17581 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    17641 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    17701 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    17761 caggctctca atatgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    17821 tttgttgctt ctacggcagg actgccggga agtgatgcgc atcacgataa accaatcgct
    17881 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    17941 ataagctaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    18001 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    18061 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    18121 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    18181 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    18241 ggcaataaag gggtagtttg ccgcgcggat accaaactgc attgccagat acagactggt
    18301 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctggt ccagattgcg
    18361 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    18421 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    18481 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    18541 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    18601 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    18661 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    18721 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    18781 aacagcatta tccattttgt tcttcccgtg atgcagacat aattctatga aagcataaat
    18841 taaaaacgca cagaagcgta gaacgttatg tctggtttat aaaatgaacc ttcaatttta
    18901 ttttttatga aaacagcatt tcatttttat ggtttcgttt ataccgatgg tttatgtgga
    18961 aattgtcgaa gagagcagat ttgcgcaacg ctgggatcag tcttaaaaag taaaaaaata
    19021 tatttgcttg aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc
    19081 agaaatgtgt ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg
    19141 gctcgtcacc gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg
    19201 ggggcaaaaa tgcctccctg ggtgaaatga ttactaatct ttccggaatg ggtgtttccg
    19261 ttccgaatgg tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg
    19321 taaaccagcg catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg
    19381 cgaaagcggg cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg
    19441 aaaacgccat ccgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcttttg
    19501 cggtgcgctc ctccgccacc gcagaagata tgccggacgc ttcttttgcc ggtcagcagg
    19561 aaaccttcct caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg
    19621 cttctctgtt taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg
    19681 gtgtggcgct ctccgccggt gttcaacgga tggtgcgctc tgacctcgca tcatctggcg
    19741 tgatgttctc cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat
    19801 ggggccttgg tgagatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtgcata
    19861 aaccgacact ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa
    19921 tccgcatggt ttacgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac
    19981 cgcaggaaca gcgtgacatc ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac
    20041 aggccgtaca aattgagaaa cactacggtc gcccgatgga tattgagtgg gcgaaagatg
    20101 gccacaccgg taaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc
    20161 aggtcatgga gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta
    20221 tcggtcatcg catcggtgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc
    20281 gcatcgaacc tggcgacgtg ctggttactg acatgaccga cccggactgg gaaccgatca
    20341 tgaagaaagc atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca
    20401 tcgctcgtga actgggcatt ccggcggtag tgggctgtgg agatgcaaca gaacggatga
    20461 aagacggtga gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg
    20521 agttgctgga atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga
    20581 aagtgatgat gaacgtcggt aacccggacc gtgctttcga cttcgcctgc ctaccgaacg
    20641 aaggcgtggg ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac
    20701 gcgcactgct tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga
    20761 tgaaaggttt tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga
    20821 cgctgggtgc cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga
    20881 acgaatatgc caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc
    20941 tcggcttccg tggcgcgggc cgctatgttt ccgacagctt ccgcgactgt ttcgcgctgg
    21001 agtgtgaagc agtgaaacgt gtgcgcaacg acatgggact gaccaacgtt gagatcatga
    21061 tcccgttcgt gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg
    21121 ggctgaaacg tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg
    21181 ccttgctggc cgagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgata
    21241 tgacgcagct ggcgctcggt ctggaccgtg actccggcgt ggtgtctgaa ttgttcgatg
    21301 agcgcaacga tgcggtgaaa gcactgctgt cgatggctat ccgtgccgcg aagaaacagg
    21361 gcaaatatgt cgggatttgc ggtcagggtc cgtccgacca cgaagacttt gccgcatggt
    21421 tgatggaaga ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt
    21481 taagcctggc tgaactgaag aaataaaata aatccccggc ggcgtttagt cgccggggtt
    21541 atgtgatccc cgaagatgaa acttattcaa tctcttcaca gacatcctgc gttaaacgcc
    21601 gcataatatc ttttcttaac aaaaactttt gtattttacc tgaggtagtt cgcggtagtt
    21661 tttcgattac cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa
    21721 aagccactac ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc
    21781 atgatcgttc acctaaacgt tcatcggaca ttgcaaccac acaggcatcg tgaattttag
    21841 gatgctgcaa taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa
    21901 taatatcttt tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat
    21961 cgccgctgta ataccagcct tcttcatcca gggcacgggc ggttaattca ggttcatcaa
    22021 aataccccat aaacacattg gggccacgcg aggcttcttc accttcacaa cctggcggta
    22081 aggtcttgcg tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg
    22141 tgtgcataaa gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg
    22201 tggaaccata aacacttaat aatttaatgc cgagctgctg gcattcacgc gccacttttt
    22261 tggggattgt ggttccgccg caaagaaaga aacgcagcgc tgaaaggtcc gcgggttgtt
    22321 tctctagtac attcaaaaga tcatagacaa acggcgttgc gcctagcata caggtgcaac
    22381 gctgctgctc aagcagcgcg agacatgcat caggagtgaa aatatctaac aacacgctgc
    22441 gagcaccaat taaaaatggt gctgttacgc catgcagaaa tcccgttgcg tgaccaagtg
    22501 gcgcaggcat cataaagaca tcctgccagg tcagattcag ccgcgcgcaa taagcccgct
    22561 cactggcgag aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg
    22621 aggtaaacag caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtggga
    22681 tattgtcggc gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca
    22741 cgcctacaat ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg
    22801 gacgcgtttg tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca
    22861 cccacaccag ttctgcttcc cgccaggaag gcaacagcgg cacggaaacc gcaccggttt
    22921 tcaggcaggc aagatagata acggtaaatt cacaccagcc tggcagttga aatgcgatgc
    22981 gatcgcctga ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat
    23041 cgagcgcgct ataggtgtac gatgcaccat gattatcgac cacggcaatt ttgtctggca
    23101 tcgcacgagc ggtctgctgc cagtaatcag ccagcgaagc atcgccccat aacccttgct
    23161 gacgatacgc cgcacgacgt tgttcgttaa acgttaatgt cactttcatt ttggactctc
    23221 gagacagaac atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa
    23281 atcaataccg aaggtgcctg cgggatattg ccactgttcg agaatagtgt tgccgcacag
    23341 cacccgacag gtgccgcatt ccagacaacc ggcagaatca aaatgaatgt ttcctgcgtc
    23401 atcctgctta taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc
    23461 gggatttgcc gccataatga tatgcgggtg gccctcatca acatggaatt tattgacgcc
    23521 taatttgatg tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg
    23581 ccatctttca gcaagttgat cagcccaatt ttcttcgcat gtcccatgat cattttgcgt
    23641 accggttggt taggcttacc gtcgatagtg aacatctcgt tcatgatgtc ggctaccatt
    23701 cgtgggtatt ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcggaaatgc
    23761 tgcatatcgc gcataacaca actttgttcc agctcacgct tgtattgcgc cagactgctg
    23821 gcggagaaat ccgtgcgttc tttggcggca atcaccgttg tggcggcagc ctgagccgat
    23881 gcaatggcta aatccatgcc gcgaactgta aaacccaaat tcaggcagaa gcctgcggcg
    23941 tcaccaacga taatcacgcc atcgttaacc agctgcggca ccatcgccag accgccttcc
    24001 ggtaccatat gcgcggagta ttcaagcagt ttgccgccgc taatcagcgg gcgaatggcg
    24061 gggtgttgtt taaaatcttc cagcatttgc ggcacacttt tttgcgcatg ggcgatatca
    24121 cccaatccac aaaccagccc caacgatacg gaatcattgt tggtatagag gaatccgcca
    24181 cccatcaggc cgtccgaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta
    24241 acattaaagc gatcgttgat ctgttctggt gtgaggccaa taacctcttt aacaccaaca
    24301 gcgtaatgat gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagtta
    24361 acgccatcag ccagaatcac cacattcgct tcgagaatat catccccagc ctgcacgcca
    24421 gtgaccttgt ttccttcacg aaccagcgca tcgacgcgaa cgcccgggat aaactgtgcg
    24481 cccgcctgct cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa
    24541 gatgcgtgtt gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct
    24601 tcggttaaga aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatactgca
    24661 aagcctggaa tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta
    24721 catccggcac tgtcgccgcg ttctatcacc aggacatcca gcccggctcg cgccatgacc
    24781 agtgcggcaa cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc
    24841 gacatgcgtt atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaaggggaa
    24901 ttagcggctc aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc
    24961 gtagtcggca tagttgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt
    25021 tgcgccgttg ccgccaacca tatgttggat ctgcccggag atccccagcg tcaggtagag
    25081 atcggatttc agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc
    25141 gccttcggca attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg
    25201 gaccattttc aggtcatcct gcgctgccag accacgaccg acgccaacca cacgttttgc
    25261 tttgctcagg tccacgctgc ttgccgcttt cgcacggcgc tcgcgacaga gaatttcatg
    25321 acgcggagca acataaggta ccgtctctgt cgggcactga tgagagttat cactggcgca
    25381 cggttcctga acaccgggtg caagggtaat gatcgccagc gagctgttga tcttttcctg
    25441 agcgaacgct aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc
    25501 caccgccgcg gcatcgttca ccagcgccgc attcagttgc acacttaacc gtgctgccag
    25561 agctttacca cgtttcgtcg cggccagtag caacatagcg gggtgtttat ctttcagcag
    25621 agcagcaatg ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac
    25681 ataaatacat tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat
    25741 ggcatacacc tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg
    25801 attatcgcta aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc
    25861 agttcagggc tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat
    25921 cgagaatgat gtgcttacgt tctgtttgcg gcggtacgcg aatgccggtc agttcagcaa
    25981 gtggtgcgct ctggctccag ccaatatcac ttgcctgcca ctgatttacc ggttttttac
    26041 ccgcgccgag aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc
    26101 agagcacggc tggaacagag agttcaataa cttcaacgtc atcttcaagc gtgcgttcaa
    26161 tcaccagtgt attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca
    26221 gaatttctcc gaccagcaag ccaacctgct gggcataaag gtcgccggaa ccttcaccaa
    26281 agatcagtaa atcgaagccg atcttttcaa ctgctgccgc cagcgctttt gcggtatcga
    26341 gaggcagtgc atgctcaagt tgtgcatcct gcaccatata caggctgtgc ggcccgcggg
    26401 atagcacgtc tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg
    26461 ctatctcatc gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact
    26521 ggctgatttt ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgc
    26581 cctgttcttc aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc
    26641 gcatacggaa ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg
    26701 tgcggggttt tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg
    26761 atcgctaaaa caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg
    26821 cattcagtgt aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca
    26881 cgccgtattt tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat
    26941 gtatcaacgc tgttttgata atgccagtga aacgctgttt gtcgctggta aaacgccacg
    27001 gctttcacgt tttgcattta gcgatgatcc aaaatgggag tctggacatc acgttcatga
    27061 caatgaaacc gagctgattt acgtcaagaa aggggtggca aggtttacca tcgattcttc
    27121 gttatatgtc gcgcatgcag atgatatagt ggtgatagaa cgcggcaggc tgcatgcggt
    27181 ggcctctgac gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt
    27241 tcagggggct gaggaaaatc atctactgca accgcattct tgtccggtaa ttgccgcggg
    27301 gcagggaaaa gaagtcatta aaaccttatt taatgagcta agtgtgattt tgccgcaaag
    27361 taaaaatagc caaacatctt cgttatggga tgcatttgcc tatacattag caattcttta
    27421 ctacgaaaac tttaaaaatg cttatcgttc ggagcagggg tatattaaaa aagatgttct
    27481 gataaaagat attcttttct atctgaataa taattatcgc gaaaaaatca ctttagaaca
    27541 gttatcgaaa aaatttcgtg ccagcgtcag ttatatttgc catgaattta ccaaagagta
    27601 tcgtatttcc ccaattaact atgttattca acggcgtatg acggaagcga aatggttact
    27661 cactaatact gaattatcac aggcagagat ctcctggcgt gtgggttatg aaaatgtcga
    27721 tcactttggc aaactgtttt tgcgccatgt cggctgttca cccagcgatt accgcaggca
    27781 atttaaaaac tgttttgcgg aacaagaaat cctatctgaa tttcctcaac cggtaagtct
    27841 tgttggataa atctggcggc gcagtttgtt aagaagctgc gccgcctgca gattatttgt
    27901 tctgatagtc tttcaggatc tgccgacctg ctacgtaaat cataatttcg tctgtaccgc
    27961 cgccgatacg ttcacaacgg acatcacgcc agaagcggga gacgcgcgct tcatcggtat
    28021 agcccagacc gcccatgatt tgaatcgcat catcaatgac ttccattgcg gtacgtgcgc
    28081 aatacagttt tgccagcgcc gcgctggtgc gcagtgactg atgctgatcg gcttgccatg
    28141 ccactttcag caccatgttg cgcatgttgt caatcttaat cgccatcagc gccagttttt
    28201 cctggatcat ctggttatga ccaatgggct taccaaaagc gatacgttgg ttggcatagc
    28261 gggcggcatc ttcaaaggcg cattcggcaa agccggtgct gcgcgcggcg ttgatcaggc
    28321 gctccatctc aaagttgtac atcacattga ggaaacccat tccttcttcg cccaccatgt
    28381 cgctctcttc aacttccacg ttgtcgagat agacttcgca ggtgctgagc atatgccagc
    28441 cgattttatg cagcgggtta atcttaatgc cgggcttact ggagtcgacc caccacaggg
    28501 tgaaggcttt tttgggatct ttcggttgcg gatcgcgcgc caacaccagc atatacgggt
    28561 actctttcgc accggtaata aaggttttct gtccgttgat gtaaaccttg ccgtttttac
    28621 gcgtgtaagt ggtggtggca ctgttgttat ctgagccagc acctggttcc gtcaacgcca
    28681 gggcataggc ggggtcgccg gtttccaggg tgctttctgc cgttttacgt agctgctctg
    28741 cagaaccgaa acggcgcata ctgtgaatac attgaccgtt ggtaattaaa aatgccggag
    28801 caccgcattt tgacacttcc atcagcgcca gcatttgggt gacgtaatcc gcagggatac
    28861 caccaaattc ttccggcacg ccaagcatgg aaataccgtt atccgccagc gcccgcataa
    28921 actcacgcgg atatgtcccg ttttgatcgc aggtgcggaa atactcttcc ggaaagttag
    28981 tcgtaatcag ttcgcgaata ctggccagca gcagttcttg ttcttcagtt aaagaaaaat
    29041 ccatcattat actcctgtta atgagccgct tcaggcagga caaaacccat cgccgcatcg
    29101 ataaataatc tttcgtccat cagtttgagt tctggcgaaa tcactggggt gaaatccatt
    29161 ttgtcgagaa tatctttttg taaatcgacg ccaggggcga tttcgattaa atgcaggccg
    29221 tcttctttca gcgtgaatac tgcgcgctca gtgatataac gaacatccag ccctcgctcg
    29281 agagcgattt ttccgctgaa agtaatttcc ggtagttccc gaataaattt cttcacccgt
    29341 ccttcctgga cgatatttaa tttgccgtcg gtaatttctg tttttaaact gcccgcagtt
    29401 aatgtgccgc agaaaatgat tttcttcgaa gtggcactga tatcaataaa tccaccggtg
    29461 cccatgattt taccattgaa tttatgcacg ccgacgttac cgtgctggtc gacttcagca
    29521 aaactcaaat aacaaacatc cagaccgcca ccgtgataaa aatcaaactg ggacgtcata
    29581 tccagaatgg cacgggtatt cacgttcgcg ccaaaggcga tcccctgtga agtaattccg
    29641 ccaatcggac ctgtttctac cgtcagaata aagtcatcag cacaaccttc atctcgggcg
    29701 accaggccaa tgccgtcagc aataccgacg ccgacattcc ccaccgcgcc tttacgcatt
    29761 tcgaataatg cgcgccgcgc aactaattta cgttggttta ggggcaggct aagtttggta
    29821 ctgtcatcaa gggtgaagtc accagaaata aagcggttaa ccggtgcacc gccatacagt
    29881 tgggtttgat ccggatcgac caccacaata tccaccagat aacccggaat acggacagat
    29941 ttaggatgca gcgtggcttt cttaaccatt ttctgcacct gcatcatcac aataccgccg
    30001 ttattgtgca ccgcctgggc aataaccaat gcgtcgagat acatcacctc atcttcaaaa
    30061 gtggcgtagc cttcgctgtc gcaggtggta gcgcgaatga aggcgatatc tggcgcaatc
    30121 gctttgtaat agagatattc tttgttatca aactcgacca gtttaatcag gtcttcttta
    30181 gtgacttcat tcagtttgcc gccttgctgg cgtggatcga caaatgtccc gatgccaata
    30241 tcactaataa taccaggctg gtgggctgcg gcggcgcgta aggtttgtgt aagtacacct
    30301 tgtgggtagt tataagcaat aattttattt tgttctgcga gttcagaaat acgcggcgat
    30361 tgtccccagt gaccacataa tgcccatttc accagacctt cttgcgccag aggactaata
    30421 ccacggtcgg cgcgatcgcc aagccctgtt ggactaataa tcgataaatt acgtggtgtt
    30481 tgagtctgtt tatatttatc agcaagagca gtaattaacg tggtggcttc cagaataccg
    30541 ccgccagcgc ctaacacaca aagtgttgct tcgtcgggaa tataattcac cgcttcctgt
    30601 gccgacagga ccggcactcg tccattaata cgaggtggtt ttacaggttt cattgttaat
    30661 tcctgattca gatgtgattt tccctacgcc aaatggagaa ttagcgcaca gagactcacg
    30721 aaatattttt attaaatacg attgaatata ttttttagtt cggcggggag ggtgttcccg
    30781 ccgaaatatt attgcttatg cctggtgtaa aatagttaat accgtgcgca aatcatttac
    30841 cgagatttgc cctggcgcag acgctttttt taccgcacca aaagttgccg ccgagccaaa
    30901 tacttcacca gccagacgag aaattacgcc agtttttgcc atcgacatcg tgataattgg
    30961 acgatcggca tactgctcct gcatctccag ggtcgcggca agcaacgtca gcacatcgct
    31021 ggtactttgc ggcatcagcg caatcttagg aatatcggcg tcgaaggatt gcattttgcg
    31081 cagacgggca atgatttctt cggcttccgg cgttttatgg aagtcatggt tggacatgac
    31141 aactttcaca tcatgcgcgt gggcgtaggc gacggtttct ttaacctgat catcgccggt
    31201 aaataactcc agatcgatca tatcaaccag gccgctgtcg atggctgcac gattgagtgc
    31261 aatataagcc tcggtggaaa tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa
    31321 cagcagcggt ttttctggca tggtctcacg gagaattttt gctgccgcca tgacagactc
    31381 cacattggag aggtcggcat agtggtccac acgccattcc agaatatcaa agtccgcttc
    31441 acgataggcg agagcttcgg atttcacgct ggcgatatct ttcgccatca gcgagacgat
    31501 gattttaggt gcgcccgtac caatgacgag atcttttaca gttacggttt tcatttttta
    31561 ccctttctgc acgcggtcag cctgtcaggc accgaacccc atgacctgtt taacatattc
    31621 cagagggaaa tctttgccag tccataatgt gaactgttca gccccttgcc acaacaacat
    31681 gccgtatcca tcaatcgttt tgcaaccagc ttgttgcgcc tgctgcaata acttcgtcat
    31741 atgcgggtta tacacgcatt cagtgaccag aagtcccgga tgtaacagac tgatatcatt
    31801 aaccaatgat tcattctcaa ggggtttcat acccactttt gtgccattgg ttaaaatgtc
    31861 ggcggaagcc agggcttcag caaaggcttg ctgatcggcg agatcggtga ccgtgacgac
    31921 acaatcggtg ttttcattaa cccgctgcgc gaaggcgagg gctttatcga agaactcatc
    31981 ccgacggtta aagagtttaa tttcttttaa accttcaatt gccccctgcg cgccaattgc
    32041 cgttgaggca ccaccggccc ctaacagcac catcgttttg cctttgatat caaaaccgct
    32101 ctctttaatg gcgcgaatat ggcccgtgcc gtcggtgtta tagccacgca gatagccatc
    32161 atcattaacg atggtgttga tggcccccac cagtttggca gctggtgtta attcatcaac
    32221 atattcacac gccagttgtt tgttcggcat cgatacacca gttccgcgca ttttgagggc
    32281 ttttaatcct tcaattgctc caggaaagct atcgttatcc acttcgaagg ccatataggt
    32341 aaatggcaat cccgcttttt ctaaggcttg attctgcatt tcgggcgata aactgtggcg
    32401 gataggatag gccatcaacc caatcaattc gtattttgcg gtaacatcca taattaactc
    32461 ctttaacctc tatgcttaat tgtgtttaac cgggtgcaaa atttacgctc gccaaaccgc
    32521 acatcatttt caggaataat gaaaacgcgg taatagcgga taaaaacaat aattgcggta
    32581 atcagggcca gcagcgcgaa agtaaaatcg agaacaatga tatattgcag gccgatgttc
    32641 gacagataac cggtaatcag tggaataaca aagttagcca gtccacccat catcatataa
    32701 atactggtga ctttggcttt gcttttggga aaaaactctg acatgaccga aacgccgagc
    32761 tgtaaaatgc cgccagctgc tgagaaaccg ataacaaagg ctccggcatt gcacaccagt
    32821 ggagaagggt acaggtagat aatggctgct gttattgttg ccagtgcaga gttaaataca
    32881 ttagcccaga tgggccggac catttttttc agtagtgcgg caaaaataaa gacacagact
    32941 aatgagccca tactgtaata agagatggtt tttaatgcct cagcttctga cataccagca
    33001 aaagccatcg catatttggg catccatacc acaatcacat aaaaggtcga gaatgcggct
    33061 acaccgaaca gtaccgatga aacaccttcc agccagacta acggtttgct gttcatttgc
    33121 ggtaattcat tagctacgct ggcgtccacc aactggctgg ggaatttgct tttcaacagc
    33181 atcaacgtga tcagtacaaa taggatgcct ggaataatca gcccatagcc gtaccagatg
    33241 ttattgagca acatatagct caccagcatt gggtagaaca tttgtccgaa tgacaccatc
    33301 gctttaacca gtatgaccgc cgaaccagag gctttcggaa agcattccat gagcgcaggg
    33361 tagccacccg tatccagcgc tgagttagcg atacctacgc acactgccag acagtaggcg
    33421 agagttaaat tcgggcaagc gggaatacca aagaagaata gcagatacat tattactgcc
    33481 attaatatca ccgcccgacg accaaactta tcggagatca caccgaagaa taaaatactg
    33541 atcaatcgtc ccaaaccgat accggaaatt aaataggcaa tgcccgcgtt gtcagtggaa
    33601 aacttttccg ccagagatga catattttgg gcaagcgtaa taacactaat gccgtgcagg
    33661 aaatagctga agtaaataca aagaacagcc aggataaatg gcgtgctgaa agccttattt
    33721 tgagacattt tcacagctcc ttgctgaaag ataatttcgt taccgtacgt attgttctcc
    33781 gacggtttac ataattacta tactgaagat gattttccct gggtacatgg cataaagttt
    33841 atgtttccag gaatattgca tgtcatagtt tgtttttgat cgcgattttc cttagtcagc
    33901 gcagaaagag ttatttatcc gtaagatctt gcatgacctg aaaagaatat tgcattaccc
    33961 acccggagcg acattttcct ttagcgagtg atgccacgtt tctttgcggc tgcgtaagcc
    34021 gataaacagt gccagtaaaa agccgatggc agcgatggcg gtatcgaacc acataatatc
    34081 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
    34141 gcccgcactg taatagatcc ctgtagcttt acctttagcg taggggaaac gttcagccat
    34201 taatgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
    34261 tattatcacc acataaaatg tgggatgcag acagacggca aacagagcaa taaatgagat
    34321 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
    34381 gataaataca caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt
    34441 gtatgacatg cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa
    34501 ggttgccatt gagatataac catataaggt ataactggct aaatcgatta ttgagcagcg
    34561 atgttccgta gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg
    34621 gaacgtacaa cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa
    34681 ggaccagccg aaccacagtt cagcccacac taacaggctg atgattagcg gtaataaaaa
    34741 ttgcccgctg gaaacaaatg ctttaattaa aatattggct gtacccggtg agcgtggaaa
    34801 agcttccatc aaactgggat aggtgcctgc atcgagaaaa ctgtttgcca ttcccgccag
    34861 aaagcccaaa ccataagcga taatgatgtt attggtgtgc aggatgccaa aaaagaaggc
    34921 catatagcag cacattccga gcatgataaa agggcggcga ccaaagcgat cggataataa
    34981 tcctgcaaaa agcaagacac ttaatcgacc aatacccagc gatgagataa ctattgagac
    35041 gcctgcggca ttagtctgcc aaagcgtctc cagcgaggcc atattcaggc tcatcaaaat
    35101 gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggaaataggg
    35161 atttttcata ggttctactt agcaaaggtg ctttaaaaag tgaagcgatg ttgtgtcagc
    35221 atccgttcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg
    35281 ctccagatcg tgtgcatata attcagcagc taccgattga tagatcttcc agtcaataaa
    35341 attgccatca gggtagactc gttgaaatgc ggttaagtcg gggaaaaaac gcatggtgtt
    35401 gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt ttctttgctg
    35461 acgcatagcc aacagttgct cgactacacg atcaggaatg gcacacttgc cattttccca
    35521 ctgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt
    35581 catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca
    35641 tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca
    35701 cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg
    35761 ttaaaaaaaa gcccatcgtg ggagatgggc aaagactaca cacagcaatt cgttgtttca
    35821 ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta
    35881 ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat
    35941 ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca
    36001 aatatcaatt agcggtggtt aacaatcagg cggcagagcc gcctgatgag aaattactta
    36061 ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggcggc
    36121 gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca
    36181 ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gagaatcagg
    36241 atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg
    36301 ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc agtccagtag
    36361 agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg
    36421 atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg
    36481 ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc
    36541 tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg
    36601 cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc
    36661 agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg
    36721 gtggtgccaa tataagggcg gactttcgcc atgatcgccg tcccccccat atccagcaag
    36781 ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga
    36841 tcgggtaacg tcatgtcacc gctggaaatg gctttaatta gcgggccgct gccgtcgacg
    36901 atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc
    36961 accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc
    37021 caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc
    37081 cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca
    37141 tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt
    37201 cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt
    37261 aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa
    37321 ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg
    37381 ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc
    37441 ctgcaacacg aaatccatag agtaaacaaa catcaccttt cagtgcgcta ttgcagcagc
    37501 ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt
    37561 ccacagattt cccaggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
    37621 gagcaacaag gttttaccgg cgatacggcg acaagttatg ccgatcgtct gacaatgtcg
    37681 accgacaaca gtatttacca acttctccct gatgcggtgg tatttccgcg ttcaaccgca
    37741 gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc
    37801 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
    37861 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
    37921 gaggccgggg tgataaaaga tcaactcaat cagtacctga aaccgttcgg ctactttttt
    37981 gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca
    38041 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
    38101 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgaatt ggcggaaacg
    38161 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
    38221 cagcaacgcc agttaattat cgacaacttc cccaaactta accgctttct taccggttac
    38281 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
    38341 ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac acgcttgcct
    38401 aaagtgcgcc gtctggtgaa cgtcaaatat gactcttttg actctgcgct gcgtaacgcg
    38461 ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat
    38521 ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tgtgcctgac
    38581 caagagatgc tcgggctgaa cattgtggaa tttgctggtg atgatgaggc gctgattgat
    38641 gagcgggtaa atgcactctg tgcgcggttg gatgagctga tcgccagtca tcaagcaggt
    38701 gtcatcggct ggcaggtgtg ccgtgagctg gcgggggttg aacgtatcta tgcaatgcgc
    38761 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct
    38821 gaggatacct gcgtaccgcc ggaacacctg gcggattata ttgctgaatt tcgcgcgctg
    38881 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
    38941 gtccgtccag cgctggatat gtgcgatcct caacaagaga ttttgatgaa gcaaatctct
    39001 gatgacgtgg tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa
    39061 ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc
    39121 aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca
    39181 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg
    39241 cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt
    39301 ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac
    39361 cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg tttgttggcg
    39421 gatcgcggcg ttgatccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg
    39481 cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc
    39541 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcgtg ttcgacccag
    39601 tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc
    39661 cgttatttac gcccgctgcg cgaccacctc gtcgctacgg tcgagagcta cgcgccgctg
    39721 atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc
    39781 tcggaaaaac atatcggcat ggttgatttg ccgctgctgt cggtcccctc gctacaacaa
    39841 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaatc tctcaatgca
    39901 gagcagaaag cgcgcacagt gttggtggtg caggacccct ttaccagcta ttacgatgcg
    39961 caagtggtgg cggattttgt ccgtctggtc gaaaaattag gtttccagcc tgtgttactg
    40021 ccattttcgc caaatggcaa agcccagcat attaaaggtt ttcttaatcg ttttgcgaag
    40081 acggcgaaaa agacggcgga tttcctcaac cgtatggcga agctgggtat gccaatggtg
    40141 ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag
    40201 gagcgtggcg agtttaacgt cttactggcg aatgaatggc tggcaagcgc acttgagtca
    40261 cagccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaagtt
    40321 accgccttgc cgggtgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa
    40381 ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa
    40441 aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg
    40501 ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa
    40561 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
    40621 ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg
    40681 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
    40741 ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta
    40801 tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa
    40861 tcaatgctaa ccacgtccgc tcggcacgag aagggcgggt gcgcggcgta tgcaaaccgt
    40921 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
    40981 tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga
    41041 cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
    41101 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt
    41161 tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag
    41221 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact
    41281 ttgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac
    41341 ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat
    41401 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    41461 ttattcttat cgccccttca agagctaagc cactgagagt gccggagata agcgccggat
    41521 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    41581 tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca
    41641 cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc
    41701 gtgcctgtta acccacacat cagggtctat gcttattaaa agcaggcaaa aagcatgttt
    41761 tacggtaaag cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    41821 tatacgctga aagcgatgaa gtgaggtaaa tcgatggaca tgcattcagg aacctttaac
    41881 ccacaagatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgt
    41941 gagctggtgg caaagcagcc gggtatggtc ggcgtgcgct taggcgtgaa gcaaacgggc
    42001 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    42061 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaag cgatgccgtt tattgatggc
    42121 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    42181 gcccagaatg aatgtggctg tggcgaaagc tttggggtat aggcggtact atgtctcgta
    42241 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaat tataaagaag
    42301 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    42361 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct
    42421 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    42481 attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg
    42541 cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    42601 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg
    42661 ccattttcga ctcagtttcg gttgccacta cttatcgcga aaaactggcg gagcagggaa
    42721 ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    42781 tcggcaccgt ggtgccgggg aatgacaact tctttgccgc gcttaatgcg gcggtagcct
    42841 ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct
    42901 attttcgcat taacgcagaa aaaaccgggc agtttgagcg caccattctg gtggccgacg
    42961 aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    43021 tacacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    43081 tacaaaactg gtttcctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    43141 gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    43201 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    43261 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    43321 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    43381 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    43441 agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgttgagt
    43501 gtcgtaacaa tagtgcgcaa ctggaacacg aggcaacgac atcacgtatt ggtgaagatc
    43561 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta
    43621 acggtttctg caaagacgtg ttctcggagc tgccgttgga atttgccgtt gaagcacaaa
    43681 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    43741 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg cggattaagc ctcgacgttc
    43801 atcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg
    43861 caacgcttgc cgggcgagaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca
    43921 aagatttgct tgcgctgtcg ccggaagatc gcgcgggcga aggcatcttt atggccttcc
    43981 agtatccggt ggaaattccc ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    44041 cggtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    44101 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    44161 gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    44221 agttatgcat tcttgatgag tcagactccg ggctggatat tgacgcatta aaagtggttg
    44281 ccgatggcgt gaactcgctg cgtgacggca agcgttcatt catcattgtt acgcactacc
    44341 aacgcattct cgactacatc aagcctgatt acgtccatgt gctgtatcag ggacgaattg
    44401 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    44461 ccgaacagca gtaacgcgct gcaacagtgg catcacttgt ttgaagctga cggagcgaaa
    44521 cgctctccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    44581 aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagcca gtttgtcagc
    44641 attgcgggag agatatcccc acagcagcgt gatgccttag cgttaacgtt agactccgtg
    44701 cggctggtgt ttgtcgatgg gcgttacgtg cccgcactga gcgatgcaac tgaaggcagc
    44761 ggatatgaag tgagcattaa cgacgaccgt cagggtttac ccgacgctat tcaggcggaa
    44821 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    44881 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    44941 gaggtgaaca ctgcccatta ccgacatcat ctggatctgg cggaaggtgc cgaagcaacg
    45001 gtgatcgaac attttgtcag cctgaatgat gctcgtcatt ttaccggggc acggttcact
    45061 atcaacgtcg cagcgaatgc ccacttgcag catatcaagc tggcgtttga aaacccgctc
    45121 agtcaccact ttgctcataa cgatttgttg ctggctgagg atgccaccgc atttagccac
    45181 agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa
    45241 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc
    45301 cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    45361 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    45421 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    45481 acaaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    45541 gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat
    45601 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    45661 gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga
    45721 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt
    45781 tgccgctggc ttatctcgac agcgccgcca gtgcacagaa accgggccag gtgattgacg
    45841 ccgaggccga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    45901 gcgctcaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    45961 cccgttcggc ggaagagctg gtgttcgtcc gcggcacgac ggaagggatc aatctggtcg
    46021 ccaatagctg gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg
    46081 agcaccacgc taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc
    46141 gtgtgatccc tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg
    46201 atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgctggga acagaaaatc
    46261 cgctggcgga aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg
    46321 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgat ttttacgtgt
    46381 tttccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct
    46441 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    46501 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    46561 ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaataaca
    46621 tagccgagta tgaacagaat ctgatgcact acgcgctatc acagctggaa tctgtaccgg
    46681 atctcactct ctatggccca caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    46741 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    46801 atcactgcgc tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc
    46861 tggccatgta taacacccat gaagaagtgg atcgtctggt gaccggattg caacgtattc
    46921 accgtctgct gggataacag ggaggcacta tggctgtatt gccggataaa gaaaaattgc
    46981 tgcgtaattt tttacgctgc accaactggg aagagaaata tctctacatt attgagctgg
    47041 gccagcgtct gccagaatta cgcgacgaag accgaagccc acaaaatagc attcagggct
    47101 gccagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg
    47161 gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc
    47221 agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc
    47281 tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc
    47341 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    47401 ttgccggatg gtcaatgttt acactgacct gcacaggagt gttttggttt caggtgaaca
    47461 taagcgccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    47521 cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    47581 gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat
    47641 acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga tactgcggca
    47701 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaagcccgg tacgacgata
    47761 actattcctt cgcaactgtt attacctgat gcgccgcgtc aggggattat cgttaacctt
    47821 gcagagctgc gcctttatta ttatccgccg ggagaaaata tcgtgcaggt ttatccgata
    47881 ggtattggct tgcaggggct ggaaacgccg gtgatggaaa cgcgtgtggg gcagaaaatc
    47941 cctaacccaa cctggacgcc tacggcaggc attcgtcaac gttcgctgga gcgtggcatt
    48001 aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc
    48061 ctcgcgcatg gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt
    48121 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc
    48181 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa
    48241 cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac
    48301 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct
    48361 gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg
    48421 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    48481 gaaccagagc aagggaatat gttacgcgcg acgcagtagc ggtaaacggc agacaaaaaa
    48541 aatggcgcac aatgtgcgcc atttttcact tcacaggtac tattacttgc ggtatttagt
    48601 agccatgttg tccagacgct ggttagcacg agctgcgtca tctttagcag cctgaacgtc
    48661 ggaacgcatt gcgttcacgt cgttgctcag ctggtcaact ttagcgttca gagtctgaac
    48721 gtcagaagac agctgatcga ttttagcgtt gctggagcaa cctgccagca gagtagaacc
    48781 caggattacc gcgcccagta ccagtttagt agctttcatt attaataccc tctagattga
    48841 gttaatctcc atgtagcgtt acaagtatta cacaaagttt tttatgttga gaatattttt
    48901 ttgatgggaa ggcacttatt tttgatcgtt cgctcaaaga agcatcgaac gctgggtttt
    48961 agataaaaaa attgagagaa aacaggatgc ttccatcgga ttcatcttag ataaagtgag
    49021 attatatagt gaaatccgat ttgatttttt attgcttctg gttatcgatt aaataaaaaa
    49081 agcgcccatc agggcgcttc gatatacaaa ttaattcaca aaagcaatat tacaggacgt
    49141 gaacagatgc ggtgttagta gtgccgctcg gtaccagtgc accagaaacc ataactacaa
    49201 cgtcaccttt gtgtgccaga ccgctctgca gagccagttc tttacccaga cggtagaaat
    49261 catcagtaga agtgatctct ttaaccagct gcggcacaac gcctttgctc agtaccaact
    49321 gatgagccgt tttttcgttg gtggtcagtg ccaggatggt ggcatccggg aagtatttac
    49381 gtactgcgcg agcagattta cctccctgag tagcaaccac gatcagcgga gcatccagtt
    49441 tttcagcagt ttcaacggca ccacggcata ccgcttcggt aatgcgcagt ttacggttgt
    49501 cattgttgaa ctcgagacgg ctgttcatca cgcggtcggt acgttcgcag atggtcgcca
    49561 tgatagaaac cgcttccagc gggtatttac cttttgcgga ttcaccagac agcatcactg
    49621 catcagtacc gtcgaggatg gcgtttgcaa cgtcaccggc ttctgcgcga gtcgggcgtg
    49681 ggtttttgat catggaatcc agcatctggg tcgcagtgat aacgacttta cgtgcacgga
    49741 tacatttttc gatcatcatc ttctgggcga agataacttc ttctaccggg atttctacac
    49801 ccaggtcgcc acgcgcaacc atgatgccgt cagaggcttc gaggatttcg tcgaagttgt
    49861 tgaggccttc ctggttttcg attttggaga tgatgtggat gttttcgccg ccgtgcgctt
    49921 tcaggtgctc acggatttcg ataacgtcag aacgcttacg aataaaggaa gcagcaacaa
    49981 agtctacgcc ttgttcgcaa ccaaagatca ggtcctgttt gtctttttca gccagtgctg
    50041 gcagagcaat ggaaacgcca ggcaggttca cacctttgtt ttcgcccagg tcaccgttgt
    50101 tcagcacttt acagataact ttgttacctt caatggcggt aacttccata ccgatcagac
    50161 catcgtcaac cagtacggtg ttgccaacag acaggtcagt agtgaaacct tcatacgtta
    50221 ccgcaaccat ttcgctgttg ccgataacag atttatcagt ggtgaaagta aaggtctgac
    50281 cagctttcag agaaacgtcg ttaccgcctt ccagtttcat ggtgcggatt tccggacctt
    50341 tggtatcaag caggatagcg gcggttttac cagttttgct catcacgttg cgcagattct
    50401 gaatgcgctg accgtgttct gcatagtcac catgagagaa gttcagacgc ataacgttca
    50461 tgccagcgtc cagcatttta gctaacatct cttcagattc ggttttcggt ccgatggtgc
    50521 aaacaatttt ggtctttttc atgacagtct tagtctttaa gttgagaagg atgggagaaa
    50581 cttgctttct gggcgcactg ccggaaagcg aatccggtat tacgaaagtg gtggtgcctc
    50641 gctctaagga taggtgacat cgaaaaagcg tgcagaggaa tgtgtgcttg tgtctcagcc
    50701 caacgtgagt gcctgttttt gagtcgttga gttctacagt ccaaagtgct gaaaccattc
    50761 aagagtcaat tggcgcgcat tatacgctga aattaatcca aaaggaaaac aaaaacagcg
    50821 tttcaaaaaa tatacaactt gttagacaat aaattgttat caaagattta acgttccagg
    50881 gaaaaggtta cgcaaaaact tcactgtttg cgcaacgtct ggtgccgaag ttgtcagtgt
    50941 gaggataagt ga
//
LOCUS       DAZFZC010000023        49791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFZC010000023 DAZFZC010000000
VERSION     DAZFZC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.023"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..894
                     /gene="gltF"
                     /locus_tag="ACHKXP_001584"
     CDS             130..894
                     /gene="gltF"
                     /locus_tag="ACHKXP_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1120 domain-containing protein"
                     /protein_id="HFO7646618.1"
                     /translation="MFFKKNLTTAAICAALSVAAFSAMATDSTDTELTIIGEYTPGAC
                     TPVVTGGGIVDYGKHHNSALNPTGKSNKLVQLGRKNSTLNITCTAPTLIAVTSKDNRQ
                     STIVALNDTSYIEKAYDTLVDMKGTKNAFGLGSAPNGQKIGAATIGIDRSNGGILAAD
                     DTGEIPVDLIQTDHWSAATPTWKASSNGAFCSLTSCSAIERGYSVAKTGELTPVAITA
                     VTFPLLIDAAVNDNTILGSDETIKLDGNVTISVQYL"
     gene            1066..1740
                     /gene="yhcA"
                     /locus_tag="ACHKXP_001585"
     CDS             1066..1740
                     /gene="yhcA"
                     /locus_tag="ACHKXP_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus chaperone family protein"
                     /protein_id="HFO7646619.1"
                     /translation="MLRHITFTVFITTSMNTLATGMVPETSVLLVDEKRGEASINIKN
                     TDDHPSLLYTTIVDLPESNKSIRLIPTQPVIRVEAGQVQQVRFLLQATVPLQSEELKR
                     VTFEGIPPKDDKSSRVTVSIRQDLPVLIHPTSLPEEREAWKFLEWRKNGDQIEISNPS
                     NYVVRMTLQFKTLPSGKTGAINKTYFLPHTSTTTALTNATDTKVEFYPASRYGYRGNK
                     YVTDLK"
     gene            1761..4142
                     /gene="yhcD"
                     /locus_tag="ACHKXP_001586"
     CDS             1761..4142
                     /gene="yhcD"
                     /locus_tag="ACHKXP_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO7646620.1"
                     /translation="MLKKTLLAYTIGFAFSPPANADGIEIAAVDFDRETLKSLGVDPN
                     ISHYFSRSARFLPGEYSLAVSVNGEKKGNIATRFDENGDICLDQAFLQQAGLKIPSEE
                     KNGCYDYILSYPGTTITPLPNQEALDIIVSPQAIIPIGLDLTNAATGGTAALLNYSLM
                     SSRAEFSNGSSDYSQAALEGGININDWMLRSHQFLTQTNGTFSNQNSSTYLQRTFTDL
                     KTLMRAGEVNLNNSVLEGASIYGIEIAPDNALQTSGSGVQVTGIANTSQARVEIRQQG
                     VLIHSILVPAGAFTIPDVPVRNGNSDLNVTVVETDGSSHNYIVPSTLFNQHVESFQGY
                     RFAIGRVDDDYDESPWVISASSGWNLTRWSAMNGGVIVAENYQAASIRSSLVPLPDLT
                     VSSQISTSQDTKDSLQGQKYRLDANYNLPFSLGLTTSLTRSDRHYRELSEAIDDDYTD
                     PTKSTYALGLNWSNSILGGFNISGYKTYSYDGDNDSSNLNINWNKAFKHATVSVNWQH
                     QLSASENNEDDGDLFYVNISIPFGRSNTATLYTRHDDHKTHYGTGVMGVVSDEMSYYV
                     NAERDHDERETSLNGSISSNLHYTQVSLAAGASGSDSRTYNGTMSGGIAVHDQGVTFS
                     PWTINDTFAIAKMDNNIAGVRITSQAGPVWTDFRGNAVIPSIQPWRTSGVEIDTASLP
                     KNVDIGNGTKMIKQGRGAVGKVGFSAITQRRALLNITLSDGKKLPRGVAIEDSEGNYL
                     TTSVDDGVVFLNNIKPDMVLDIKDEQQSCRIHLTFPEDAPKDVFYETATGECQ"
     gene            4139..4684
                     /locus_tag="ACHKXP_001587"
     CDS             4139..4684
                     /locus_tag="ACHKXP_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcE family protein"
                     /protein_id="HFO7646621.1"
                     /translation="MKRIITGCLLLNFAMAAQAECNISSSIQNIDYGKRSAAMRQVDR
                     GKTTQLADRTITLVMQCDQDAHIRVQLNTANISNNGFGFGPNGSLNLIASDAFSGSNN
                     LDLALASGKNDNPGSTGTASISTSPNNWLVFMQNGQEVVIDSGKSVSLTLTMAPAFKD
                     EGELTDMTDITGNLTVLVEAK"
     gene            4681..5397
                     /gene="yhcF"
                     /locus_tag="ACHKXP_001588"
     CDS             4681..5397
                     /gene="yhcF"
                     /locus_tag="ACHKXP_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1120 domain-containing protein"
                     /protein_id="HFO7646622.1"
                     /translation="MNNVKLLIAGSAFFAMSAQAADRVSIDVKVTLEAAACTPILSNG
                     GVVNFGSHSVNRLSTQHYTQIGTRNINMTITCESATGIAITARDTRMDSMTTGKDSGG
                     QSGVKYTLNGGGYISQTTRLFGLGKTKDNKNIGSYAVLIDSNNISASNGSQTLAVSIA
                     GADAVITGQKRAWQTLTAYPLAVDQSYYYTFVKPGETTPTPVTNAIIPLQVSASIAND
                     LGGSEKIELDGKAVISVVYL"
     gene            5582..6709
                     /gene="yhcG"
                     /locus_tag="ACHKXP_001589"
     CDS             5582..6709
                     /gene="yhcG"
                     /locus_tag="ACHKXP_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO7646623.1"
                     /translation="MESLSEGTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYQE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLCLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTMSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPRSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(6769..7233)
                     /gene="nanQ"
                     /locus_tag="ACHKXP_001590"
     CDS             complement(6769..7233)
                     /gene="nanQ"
                     /locus_tag="ACHKXP_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO7646624.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7230..8105)
                     /gene="nanK"
                     /locus_tag="ACHKXP_001591"
     CDS             complement(7230..8105)
                     /gene="nanK"
                     /locus_tag="ACHKXP_001591"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO7646625.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEFQALDGDITDMVFITVSTGVGGGVVSGCKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8102..8791)
                     /gene="nanE"
                     /locus_tag="ACHKXP_001592"
     CDS             complement(8102..8791)
                     /gene="nanE"
                     /locus_tag="ACHKXP_001592"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO7646626.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(8839..10329)
                     /gene="nanT"
                     /locus_tag="ACHKXP_001593"
     CDS             complement(8839..10329)
                     /gene="nanT"
                     /locus_tag="ACHKXP_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO7646627.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(10439..11332)
                     /gene="nanA"
                     /locus_tag="ACHKXP_001594"
     CDS             complement(10439..11332)
                     /gene="nanA"
                     /locus_tag="ACHKXP_001594"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7646628.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(11454..12245)
                     /gene="nanR"
                     /locus_tag="ACHKXP_001595"
     CDS             complement(11454..12245)
                     /gene="nanR"
                     /locus_tag="ACHKXP_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO7646629.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            12625..13992
                     /gene="dcuC"
                     /locus_tag="ACHKXP_001596"
     CDS             12625..13992
                     /gene="dcuC"
                     /locus_tag="ACHKXP_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7646630.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(14035..14532)
                     /gene="sspB"
                     /locus_tag="ACHKXP_001597"
     CDS             complement(14035..14532)
                     /gene="sspB"
                     /locus_tag="ACHKXP_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO7646631.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEETSADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(14538..15176)
                     /gene="sspA"
                     /locus_tag="ACHKXP_001598"
     CDS             complement(14538..15176)
                     /gene="sspA"
                     /locus_tag="ACHKXP_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO7646632.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(15571..15963)
                     /gene="rpsI"
                     /locus_tag="ACHKXP_001599"
     CDS             complement(15571..15963)
                     /gene="rpsI"
                     /locus_tag="ACHKXP_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO7646633.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(15979..16407)
                     /gene="rplM"
                     /locus_tag="ACHKXP_001600"
     CDS             complement(15979..16407)
                     /gene="rplM"
                     /locus_tag="ACHKXP_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO7646634.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(16626..17753)
                     /gene="zapE"
                     /locus_tag="ACHKXP_001601"
     CDS             complement(16626..17753)
                     /gene="zapE"
                     /locus_tag="ACHKXP_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO7646635.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            17947..18345
                     /gene="zapG"
                     /locus_tag="ACHKXP_001602"
     CDS             17947..18345
                     /gene="zapG"
                     /locus_tag="ACHKXP_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO7646636.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            18499..19866
                     /gene="degQ"
                     /locus_tag="ACHKXP_001603"
     CDS             18499..19866
                     /gene="degQ"
                     /locus_tag="ACHKXP_001603"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO7646637.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            19956..21023
                     /gene="degS"
                     /locus_tag="ACHKXP_001604"
     CDS             19956..21023
                     /gene="degS"
                     /locus_tag="ACHKXP_001604"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO7646638.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(21086..22024)
                     /gene="mdh"
                     /locus_tag="ACHKXP_001605"
     CDS             complement(21086..22024)
                     /gene="mdh"
                     /locus_tag="ACHKXP_001605"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7646639.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            22459..22929
                     /gene="argR"
                     /locus_tag="ACHKXP_001606"
     CDS             22459..22929
                     /gene="argR"
                     /locus_tag="ACHKXP_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO7646640.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            23294..23557
                     /gene="yhcN"
                     /locus_tag="ACHKXP_001607"
     CDS             23294..23557
                     /gene="yhcN"
                     /locus_tag="ACHKXP_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO7646641.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(23613..23885)
                     /gene="yhcO"
                     /locus_tag="ACHKXP_001608"
     CDS             complement(23613..23885)
                     /gene="yhcO"
                     /locus_tag="ACHKXP_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO7646642.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23977..25944)
                     /gene="aaeB"
                     /locus_tag="ACHKXP_001609"
     CDS             complement(23977..25944)
                     /gene="aaeB"
                     /locus_tag="ACHKXP_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO7646643.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(25950..26882)
                     /gene="aaeA"
                     /locus_tag="ACHKXP_001610"
     CDS             complement(25950..26882)
                     /gene="aaeA"
                     /locus_tag="ACHKXP_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO7646644.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(26890..27093)
                     /gene="aaeX"
                     /locus_tag="ACHKXP_001611"
     CDS             complement(26890..27093)
                     /gene="aaeX"
                     /locus_tag="ACHKXP_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO7646645.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            27276..28205
                     /gene="aaeR"
                     /locus_tag="ACHKXP_001612"
     CDS             27276..28205
                     /gene="aaeR"
                     /locus_tag="ACHKXP_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO7646646.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(28339..29784)
                     /gene="tldD"
                     /locus_tag="ACHKXP_001613"
     CDS             complement(28339..29784)
                     /gene="tldD"
                     /locus_tag="ACHKXP_001613"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO7646647.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(30214..34014)
                     /gene="yhdP"
                     /locus_tag="ACHKXP_001614"
     CDS             complement(30214..34014)
                     /gene="yhdP"
                     /locus_tag="ACHKXP_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO7646648.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVNNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGSDIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINSDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNIELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(34082..35551)
                     /gene="rng"
                     /locus_tag="ACHKXP_001615"
     CDS             complement(34082..35551)
                     /gene="rng"
                     /locus_tag="ACHKXP_001615"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO7646649.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(35541..36134)
                     /gene="yhdE"
                     /locus_tag="ACHKXP_001616"
     CDS             complement(35541..36134)
                     /gene="yhdE"
                     /locus_tag="ACHKXP_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO7646650.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(36143..36631)
                     /gene="mreD"
                     /locus_tag="ACHKXP_001617"
     CDS             complement(36143..36631)
                     /gene="mreD"
                     /locus_tag="ACHKXP_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO7646651.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(36631..37734)
                     /gene="mreC"
                     /locus_tag="ACHKXP_001618"
     CDS             complement(36631..37734)
                     /gene="mreC"
                     /locus_tag="ACHKXP_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO7646652.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(37800..38843)
                     /gene="mreB"
                     /locus_tag="ACHKXP_001619"
     CDS             complement(37800..38843)
                     /gene="mreB"
                     /locus_tag="ACHKXP_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO7646653.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(39148..41088)
                     /gene="csrD"
                     /locus_tag="ACHKXP_001620"
     CDS             complement(39148..41088)
                     /gene="csrD"
                     /locus_tag="ACHKXP_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO7646654.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSVLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESAMRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            41240..42214
                     /gene="yhdH"
                     /locus_tag="ACHKXP_001621"
     CDS             41240..42214
                     /gene="yhdH"
                     /locus_tag="ACHKXP_001621"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO7646655.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRVLPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            43192..43662
                     /gene="accB"
                     /locus_tag="ACHKXP_001622"
     CDS             43192..43662
                     /gene="accB"
                     /locus_tag="ACHKXP_001622"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO7646656.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPVTVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            43673..45022
                     /gene="accC"
                     /locus_tag="ACHKXP_001623"
     CDS             43673..45022
                     /gene="accC"
                     /locus_tag="ACHKXP_001623"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO7646657.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            45131..45373
                     /gene="yhdT"
                     /locus_tag="ACHKXP_001624"
     CDS             45131..45373
                     /gene="yhdT"
                     /locus_tag="ACHKXP_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO7646658.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            45363..46814
                     /gene="panF"
                     /locus_tag="ACHKXP_001625"
     CDS             45363..46814
                     /gene="panF"
                     /locus_tag="ACHKXP_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO7646659.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            46826..47707
                     /gene="prmA"
                     /locus_tag="ACHKXP_001626"
     CDS             46826..47707
                     /gene="prmA"
                     /locus_tag="ACHKXP_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO7646660.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            48036..49001
                     /gene="dusB"
                     /locus_tag="ACHKXP_001627"
     CDS             48036..49001
                     /gene="dusB"
                     /locus_tag="ACHKXP_001627"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO7646661.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            49027..49323
                     /gene="fis"
                     /locus_tag="ACHKXP_001628"
     CDS             49027..49323
                     /gene="fis"
                     /locus_tag="ACHKXP_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO7646662.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            49409..>49791
                     /locus_tag="ACHKXP_001629"
     CDS             49409..>49791
                     /locus_tag="ACHKXP_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO7646663.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMY"
BASE COUNT        12489 a        12960 c        12312 g        12030 t
ORIGIN      
        1 attaaataaa tacatcccag caagttggct atttctgaac gagttattta tttacattca
       61 caaacagata tatatactgc ttcagctttt tattttaaaa aataaatttg ttattaagga
      121 tatgttcata tgtttttcaa aaagaacctc acaaccgctg ctatttgtgc agcactttct
      181 gttgcagcat tcagtgccat ggcaacagat tcaactgata cagaattaac tatcatcggc
      241 gagtataccc ctggtgcatg taccccagtt gttacgggcg gcggcattgt tgactatggg
      301 aaacatcata acagtgcgct gaatccgacc ggtaaaagta ataaactggt tcaactgggc
      361 cgcaaaaaca gcacattaaa tataacctgc acagcaccaa cgctaattgc tgtaacctcc
      421 aaggataacc gtcagtcgac cattgtagcg ttaaacgaca catcctatat tgaaaaagct
      481 tatgatactc tcgtagatat gaagggaacg aaaaacgcct ttggtttagg ttctgcgcct
      541 aatggacaaa aaattggcgc tgcaaccatt ggtattgaca ggtctaatgg cgggattctc
      601 gcggcggacg atacaggtga aattcctgtc gacctgattc aaactgacca ctggtcagca
      661 gcgaccccca catggaaagc ctcgtctaat ggcgcattct gctcgctgac aagttgctca
      721 gcaatcgagc gcggttattc tgttgcaaaa acaggggaac tgacacctgt tgccattaca
      781 gcggtcacct tcccgttact gattgatgct gcggtaaatg acaatactat tcttggttct
      841 gatgaaacga tcaaactgga tggtaacgtc actatcagcg ttcagtattt ataagaactt
      901 gtaatacagt ttttattttt acgcattaac cagttagcta taactcggac agattatgcc
      961 ctatgacatg gtctgtctta atcaatctca tcgcaaacag tgcataacat atattaacaa
     1021 tatgtttctg cgttatttat cattattaca ggagttttaa tttaaatgct cagacacatt
     1081 acattcactg tatttataac aacatcaatg aataccttag ccacagggat ggttcctgaa
     1141 acatcagtat tgctggttga tgaaaagcgt ggtgaagcta gtattaatat taagaataca
     1201 gatgatcatc cttcattgct ttacaccacc attgttgatt tgcctgaaag caataaatca
     1261 attcgtttaa tcccgactca gcccgttatt cgtgttgaag ccggtcaagt acaacaggtt
     1321 cgatttttac ttcaggcgac tgttcctctg caatcagaag agctgaaacg cgtaacgttc
     1381 gaaggtattc caccaaaaga tgacaagagc agcagagtta ctgtttcaat tcgtcaggat
     1441 ctacctgtac tgatccaccc gacttccctg ccggaagagc gagaggcctg gaaattcctg
     1501 gaatggcgta aaaatggcga ccaaattgag attagcaacc ccagtaatta tgtcgttcgt
     1561 atgaccttac aatttaaaac gctcccttca ggaaaaacgg gggctataaa taagacctat
     1621 tttctaccac acaccagtac aactactgca ttaacaaacg ctacggatac aaaagtcgag
     1681 ttttacccag ccagccgata tggttatcgc ggtaataaat atgtcactga cttaaaataa
     1741 ctttgcctgg agcgacaagg atgttaaaaa aaacgttact ggcctacacc attggttttg
     1801 ccttttctcc cccagccaat gcagatggta tagagattgc cgctgttgat tttgatcggg
     1861 aaacattaaa atcactaggt gtagatccta atatatcgca ttatttttcc cgttcagccc
     1921 gttttttgcc aggtgaatat tcactggcag tatcagtaaa tggcgaaaaa aaaggcaaca
     1981 ttgctacgcg ctttgatgaa aatggcgaca tttgtcttga tcaggcattt ctgcaacaag
     2041 ccggtttaaa aattccttct gaagaaaaaa atggctgtta cgactatata ttgtcctacc
     2101 cgggtaccac aatcacacca ttacctaacc aggaagcgtt agatattatc gtttcaccac
     2161 aggcgatcat tcccataggg ttggatctca caaacgcagc aactggtgga acagctgcgc
     2221 tgctaaacta ctctctgatg agcagccgtg cagaattttc taatgggagt tcggactact
     2281 cccaggctgc acttgaaggc gggattaata ttaatgactg gatgttacgc agccatcagt
     2341 tccttacaca aacaaatggc acattcagta accagaactc gtcaacctac cttcaacgta
     2401 cctttacaga tcttaaaaca ctcatgcgag caggtgaagt taacctcaat aatagcgtgt
     2461 tggaaggagc cagtatttac ggtatcgaaa tcgcaccgga caacgcattg caaaccagcg
     2521 gcagtggtgt gcaagttact ggtatagcca acacctctca ggctcgtgtc gagattcgtc
     2581 aacaaggagt tttaattcat tccattctgg ttcctgcggg cgcattcact atccctgatg
     2641 tacctgttcg caatggtaat agtgatctta atgtcaccgt tgtcgaaaca gacggtagtt
     2701 cgcacaacta tattgttccc tccaccctgt ttaatcagca tgtagaaagc ttccagggtt
     2761 atcgcttcgc gatagggcgg gtagacgatg actatgacga atcaccttgg gtaattagtg
     2821 catcgagcgg atggaatctg acacgctgga gtgcaatgaa cggcggcgtt atcgtagcag
     2881 aaaattatca ggcggcatca atccggtcga gtctggttcc cctgcccgat ttaacagtga
     2941 gcagccaaat tagtacatcg caggatacga aagactcact gcaaggacag aaatatcgtc
     3001 ttgacgcgaa ctacaatctc ccattttcac ttgggctaac aaccagcctc actcgatctg
     3061 atcgccatta tcgcgaactg tctgaagcga ttgatgatga ttataccgat ccgactaaaa
     3121 gcacttatgc gcttggttta aactggtcta actccattct gggtggtttc aacatcagtg
     3181 gctataaaac atatagttac gacggtgaca atgactcaag caaccttaat attaactgga
     3241 acaaagcgtt caaacacgcc acggtttccg tcaactggca gcatcaactt agtgcttcag
     3301 aaaataatga agacgatggt gatctgttct acgtcaacat cagtattcca tttggcagat
     3361 caaacaccgc cacactgtat actcgccatg acgatcataa aacccactat ggtactggtg
     3421 tcatgggagt cgtctcagat gagatgtcct actatgtgaa tgctgaacga gatcacgacg
     3481 aacgtgaaac gagcttgaac ggcagtatca gttccaatct ccattacacc caagtcagcc
     3541 ttgccgcagg agcaagcggc agtgatagcc gtacttacaa cggtacgatg tcaggtggta
     3601 ttgccgtaca tgatcaggga gtgacctttt caccgtggac tatcaatgac actttcgcca
     3661 tcgcaaaaat ggataacaat attgcaggtg tcagaattac atctcaggca ggcccagtat
     3721 ggacagattt tcggggtaat gccgtcattc catcaatcca gccgtggcga acatcaggag
     3781 ttgagatcga taccgccagc ttgccaaaaa atgtcgatat cggtaacggc acaaaaatga
     3841 tcaaacaagg ccgtggtgca gtagggaaag tcggattcag tgcgataaca caacgccgtg
     3901 cattactcaa tatcacactt tccgacggca aaaaactgcc cagaggcgtt gcgattgaag
     3961 atagtgaagg caactatctg acaacatcag tggatgacgg tgttgtattc ctcaataaca
     4021 tcaaaccgga catggtgcta gatataaaag atgagcagca atcatgccgt attcacctta
     4081 cattcccaga agatgcacca aaagatgtgt tctatgagac agcaacagga gagtgccaat
     4141 gaaacggata ataacaggat gcctgctgct gaacttcgcc atggccgctc aggcagagtg
     4201 taatatctcc agtagcatac aaaatatcga ctatggcaaa cgaagtgctg ccatgcgcca
     4261 agttgaccgg ggtaaaacaa cacaattagc agacagaaca atcacgctgg taatgcaatg
     4321 cgatcaggac gctcatattc gtgttcagtt gaataccgct aatatttcca acaacggatt
     4381 tggatttggc ccgaatggtt cattaaatct gatcgccagc gatgcatttt caggtagtaa
     4441 caatctcgac ctggcgttag ccagtggcaa aaatgacaat ccgggcagca caggaacagc
     4501 atcaattagt acttccccca ataattggct ggtgtttatg caaaatggtc aagaagtggt
     4561 aattgatagc ggtaaaagcg ttagcctcac actgacaatg gctccagctt ttaaggatga
     4621 aggggaacta accgacatga cagatattac aggcaatctg acggtcctgg tggaggcaaa
     4681 atgaacaatg taaaattact gattgccgga agtgcctttt ttgccatgtc agcgcaagcc
     4741 gctgatagag tatcaattga cgttaaggtg actctggaag ctgcagcttg tactccaata
     4801 ctaagcaacg gcggagttgt taacttcggt agtcattcag tcaatagact ttcaacgcag
     4861 cactacacac agattggaac acgtaatatt aatatgacta taacttgcga atccgctacc
     4921 ggcattgcga ttaccgctcg tgatacacga atggatagta tgaccaccgg aaaagatagt
     4981 gggggtcaga gtggtgtaaa atatacctta aatggcggag gttatattag ccagacaacg
     5041 cgcttattcg gcttagggaa aacaaaagat aataaaaata tcggtagtta tgccgtgtta
     5101 attgatagca acaacattag tgccagcaat ggtagccaga cgttagctgt atcaatagca
     5161 ggtgcagatg ccgtcataac agggcaaaaa agagcatggc aaacgctcac cgcttatccg
     5221 cttgcagtcg atcaaagcta ttattacacc tttgtaaaac caggtgaaac aaccccaact
     5281 cctgtaacga acgccattat accgcttcag gtgagcgcat ctatcgcgaa tgatttaggc
     5341 ggttcagaaa aaatcgaact tgatgggaaa gctgtgataa gcgtagtcta cttgtgataa
     5401 caatgccgga caggagtact atcaggcatt cgttttatac gatcggttaa tgtttcagga
     5461 aagctcttcg catatcttgg ggaaagtaaa tgtaatacgc tgttcttaat tgttcagtgc
     5521 tcacccccca gagtatagct cattccatta ttcaacttag ctccatataa agggacagat
     5581 aatggaatcg ctctcagaag gaaccacagc aggctaccag caaatccacg acggtattat
     5641 tcatctggtc gatagcgccc ggacggaaac ggtacgtagc gttaacgcgt taatgaccgc
     5701 gacgtaccag gaaattggcc gacgaattgt cgaatttgaa caaggtggcg aggccagggc
     5761 tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat ttatgtctaa ggtataagcg
     5821 tgggttctct gcaaaaaact tacgccaaat gaggcttttt tacctctttt ttcaacatgt
     5881 tgaaattcgc cagacaatgt ctggcgaatt aacaccattg ggaattccgc agacaccgtc
     5941 tgcagaattc ccatcggcaa agatttggca gacgctgtct gccaaatctt tccccttgcc
     6001 caggtccacc tacgtccgtt tactttccgt taaaaacgct gacgcccgca gcttttatga
     6061 aaaagagacg ctccgctgtg gctggtctgt tcgccagcta gagcggcaaa ttgcgaccca
     6121 gttttatgag cggacactac tgtcacatga caaatcagcc atgctgcaac aacacgctcc
     6181 tgccgagacg catattcttc cgcaacaggc gatacgcgat ccctttgtgc tcgaatttct
     6241 ggaattgaaa gatgaatatt cagaatccga ttttgaggag gcgctgatca accacctgat
     6301 ggatttcatg ctggaacttg gggatgattt tgcctttgtt ggtcggcagc gaaggttacg
     6361 cattgatgac aactggtttc gtgtcgatct gctgtttttc caccgccgtt tacgctgcct
     6421 gctaatcgtc gatctaaaag tgggcaaatt cagctatagc gatgccggac agatgaatat
     6481 gtatctcaac tacgccaaag agcactggac gctaccggat gaaaatccgc ccatcggtct
     6541 ggttctctgt gcagagaaag gagccggaga agcgcattat gctctggcag gtttgcctaa
     6601 caccgttctg gcaagcgaat ataagatgca actacctgat gagaaacgac tcgcagatga
     6661 actcgttcga acacaggcgg tgctagagga aggctataga cgccgttaat ttcagcggtt
     6721 tcgaattctg cagacagtgt ctgcagaatc tttaaaacga actaagtttt aagccattaa
     6781 atcagcctta accttcacca caaccttttt aatctctcca ggctcgccga caacgcatcc
     6841 tggtttatgc ggttcacctg gcataaacac ggcgaacatt cccggtttta agatgatggc
     6901 ttgctcgtta tcaatggtgc tgcaaagctg ataatcatcc tcatggtgga actcttcaca
     6961 ctgacgcgca gtgcctgcca tgccaaacag aatccgttcc tcaccgttta ataacagctg
     7021 gatatcaatg tattgctcgt gcaattccgc ttttttctcg acgggcgatt gagtgttaaa
     7081 cgtcatgaca ttcataaaga tattgtcgcc ctgtaattcg taacgacccg gcgctttttc
     7141 ttgcggtctg gcagctaatg ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc
     7201 agacggtaat gactgtactt cacccatcat cataattttt ctccctgggc caacagcgca
     7261 gccccaagta aacctgcatc atggcggtaa tgcgccgcca gtaaatcaac atgaaatgcc
     7321 gctggctcct gcgccagata cgtttccacc agcgccagat acccttctgc cagaccaacg
     7381 ctgccaccga ccaccacgca ctggcaatca gttgtggctt taatatcagc gatcagcctt
     7441 gcaagcgtac gtgcggagcg gtgaatcagc tgctgcgcct gctcgtcacc ctgcccggcg
     7501 cgcgtgaaaa tagttttcgc atccgcgcca gccaactccc cctgcgctgc cgctgcaatg
     7561 ccgcgaccag aagcaatcgc ttccacgcaa cctgtgcgtc cacagccgca gactgggccg
     7621 tgtggatcgg caagcgtatg cccgatatgc cccgccagac cgccagggcc ggtaagcagt
     7681 ttgcagccgc tcactacacc gccgccaacg ccggtggaaa cggtgataaa gaccatatcg
     7741 gttatatcgc catccagcgc ctgaaactcc gcccatgctg cggcctgcgc gtcgttaatg
     7801 gcaatggtcg gcaaattggt aagttgttcc agcgttttga ctaacggaaa gtgtagcaat
     7861 ccaccaagat tatgcggatt cagcgccagc aaactgccgt cgcgaataat tccggttgaa
     7921 gcgatggcaa cccgctgcgc atgagcttgc aacggagaga ctaatgcgga taaggcatca
     7981 cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt cacgacgatc gcggatctgc
     8041 ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg
     8101 gtcatagcac cgcctttttc atcgctgtgt tgtaccactg acaaatgtgc tcaagacgcg
     8161 tgattgcaga accgaccgtc accgcccacg cgccgtggcg catcgcatcc gccgcctgag
     8221 caggcgtgtt gtaacgccct tcggcaatca cccgacatcc ggcgtcgctc aacgttttca
     8281 ccagcgccag atccggctct tctggcgttt caggcgtggt atagccagaa agcgtagtgc
     8341 caataatttc ggctcccagc ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg
     8401 tcatcgccag taaaccgtga tggtgaatac gtgccagcag cgtttcaaca ggcaccggac
     8461 gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcaa
     8521 catcttcaat ataggccgtg atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc
     8581 caataatcgg cacgctcacc accgcacgcg tggcttgcag atttgccaca ccttcaatgc
     8641 gaatggcaac cgcgcccgcc tgttctgccg ctaatgccat ggcggcgacg atttcgggtt
     8701 tatcgagcgg gctgtccgga accggctggc aggagacaat caggccaccg ttagcagcga
     8761 ttttttgatc cagttgtgca agtaacgaca tacatcttcc cttagcgaaa ggcccggtac
     8821 atagaccggg caacaggatt aacttttggt tttgactaaa tcgtttttgg cgctgccaaa
     8881 cggcacggca ccgctgaatg gtttaccgtc gatagcgtca tgagtacgca acgcttccgg
     8941 gcgcaaccaa cgctgaacgc gagaaggcat atccagccca atcagcagga tcaccacgaa
     9001 cgtcagactg aacgagagcg atgccagcgc agtacccaga tccagacgtt gagcgatcaa
     9061 cgcgccgatg attggggcca gtgcaccgcc caatgcgcca acgttgtagg taaagcccag
     9121 gcccgctgca cgctggtcgg tatcgaaata accgccaatc agttttggta agatcccggc
     9181 gatcccttgt ccaagcattt gctggaagaa cagtaacaga ccgagcaccc agacgtttgc
     9241 gccgccaatc gcaaataccg gaataatcag cagctgcgag gccagcaggc tacaaacgta
     9301 cgctttgcgg gttcccagcc agtcaccgag gaagccacct acgcagcatc ccaccgccgc
     9361 gccaaagcca ctaaagaaca gcacattggc tacagtatgc gggttataag ccagatcggt
     9421 tttcagatac gttggcagca gcgcctgaat cggccatgag tagaggaaag caaacaacac
     9481 gaccaccatc agcattacgc ccgttggcca gcgtttgcct gtactctgca ccataaagct
     9541 gataaagatt gcggcgcata acagcccaag aacagcgacg atcgcggcat tttgcaggtt
     9601 accggcgaag cagaaccaca gcgcagtagc cgccgccagt gtcattacga tattggcaat
     9661 gcgatgttca ccacggtaga gaatatccac cattgtgcgt actggtgctt tacctgcgtg
     9721 tttctctttc cagtcttccg cttccgggat gtttttacgc agccagagag caaagatgat
     9781 tggcaaaatg ccgataaaga acagcgcacg ccaaccccag accggaacca ccaggctata
     9841 gacctgagcg gcaacgacgg cccccacaga gaagcctgaa atcaaaaaac cactggcttt
     9901 gttacgcaga tgttttggcc agctttcaat gacataggtg gcgctggaac cgtattcacc
     9961 cgccatcccc atgccgatga ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa
    10021 gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg gtgaccattg ccagacgacg
    10081 cccgtagcgg tcacccatag cgccgagcat caggccgccg aaccagcgag agataaaggc
    10141 tgcggagatc agacttgccg cctgcaccgt cgtcagcccg aattcaccct gtacttcggt
    10201 gagtaccagg gcgattaaaa cgaaatcaaa accgtcaagc agatatccca accaggcagc
    10261 ggaaaatgcg cgccattgtg cacggttgag atggcgatac cacgggatat tctgggttgt
    10321 agtactcatt gtgagtctcc cgcggtgggc gatgcccaca cgctttggta tgaaaattgt
    10381 agggtacaga tgcgtttatt tcccctcacc cggtaggggc gagcgagggg aaaacaactc
    10441 acccgcgctc ttgcatcaac tgctgggcca gcgccttcag ttctggcaga tatttttcat
    10501 ctaccggtcc aaacggtttg cggcacagcg gcacagaaac gacatccata taatgcagga
    10561 cagttttcag gccgcggaat acgcccgttt tgatcagtaa atcaatgact ttgttgcact
    10621 cagtttgcag tttctgcgcg gtctggatat cgccttcttt cagcgccttc acgatcccct
    10681 gatatcgcca gcccatgatg ttgtaagtac tgccgatacc accatcagcg cctgccagca
    10741 agccggaagc gaagatttcg tcgtaaccgt tatagagcac cagatcagga tgttcacgac
    10801 ggatctgttc catctgatag agatcgccag aagtctgttt cagcgcaccg acgccaggca
    10861 acgtaaccag ggtgttgatc tgatccaggg tcagttttac cccactcagg gctggaatgt
    10921 tgtacaccac catcggcaaa ccatccgctg aatcaataat tgcccgatag tgatcgcagt
    10981 gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc ggagacggca tcgaagccat
    11041 aacgtttagc cgatgccgca agttgttggc tttcggcggt gctgacgcaa ccgacgtggg
    11101 cgatgagttt aatcttacct ttcgcctctt cggcgacgat ttccagtacc tgttcacgct
    11161 cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc cacgtataaa ccgtcgatgc
    11221 cctgctgaat attgaactga accaggcgac gcagactcgc tttatccagt gcttgttgtt
    11281 ggtcaaaagg agtcaggagt gcagccatta cgccacgtaa attcgttgcc ataaatacct
    11341 ctgaagtgat gcttgtctga taaacgatat acctttatac ctgttatacc agatcaatta
    11401 agcaacaccc tatacagaaa gcttataatg cgatctgctt cactaaagtg gcattatttc
    11461 tttttgttgg tggtctgacc gaaagcgtgc caggtagcag agacgctgtt gagatgcgat
    11521 tgcaacgcac gatcggcttc gtcaggatca tgacggcgga tcgcatcaac gatcgcaata
    11581 tgctgttgat aactaacgtt gttatgttcg tgcagtgcct gatcggtaac cgttgggcgt
    11641 gcggcaataa gccagtcgag cagggcaacg tggatcgcca tgaagattgg gttaccgggg
    11701 atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa tgaatgccgc gttattatcc
    11761 agcgactgac tgttgatttc cagtgctttt gccagcaaat cgatttgctc atcggtggca
    11821 tgttcagccg catagcgcac cagactggat tcaaagaaca gacgtaattg ttcgaaatgg
    11881 gcaatcccac cgggatgaga aaggaaatct ttcgccatgc cggaaagctc accgatgata
    11941 gtgtccgcag aaggacgcga gacgcgagcg cgttcgccgt tgtttatttg caccagacct
    12001 ttgcgtttta acgctgccag cgcttcacgc accgaaggac gcccgacgtt aaagaacgcc
    12061 atcagttcgc gttcagacgg taattgttca ccttcgccaa attcacgacg gcggatcatc
    12121 tgttccagct cttcttccac catttcggag agttttttac gcgccagcgg gcggctacgc
    12181 aagttgcgac caattgcagg tgaagaatct tcggtttgcg aatcaaatgc gttcataagg
    12241 cccattctgt aaggtcagtg tgattaacat catcagtgac atcctatcac aggattgaaa
    12301 gtaggggaaa atggcagggt tttctctttg tgcctcatca ttaccataat taacggaata
    12361 attaactatt gcgaaaaatt aatgtaacgc agataaaaac atcccgtttg aattatttat
    12421 aagactattc acgagcatta tgaatattat gaatgtgttc ttacaaaata atcataagcg
    12481 catatttttt aatgaaaaat cacctcacct acaattaaaa acacgacatc cgcaccataa
    12541 atagccttgc aaaaaatata acatcgttgt tttcaatctg ccgtttatgg gattgaccgt
    12601 tttcttttga cacggagttc aacaatgttc ggcataatta tatctgtcat cgtattaatt
    12661 acgatgggct atttgatcct gaaaaactac aaacctcagg tggtgctggc tgccgcaggt
    12721 atcttcctga tgatgtgcgg tgtctggtta gggttcggtg gtgtactcga tcccaccaaa
    12781 agcagcggct acttgatcgt cgatatttat aatgaaatcc tgcgcatgct gtccaaccgc
    12841 attgccggat tggggctgtc gattatggcg gtgggcggtt atgcccgcta catggagcgc
    12901 ataggggcca gtcgcgcgat ggtgagcttg ttaagccgcc cgttaaaact cattcgctcg
    12961 ccgtatatta ttctgtcggc aacttacgtc atcggccaaa tcatggcgca gtttattacc
    13021 agcgcctccg gtctgggtat gttgctgatg gtcaccttat ttccgacgct ggtgagtctg
    13081 ggagtaagtc gcctctctgc ggtggcggtt atcgcaacca caatgtccat tgagtgggga
    13141 attctggaaa cgaactccat ttttgctgcc caggtagcgg gaatgaaaat tgccacatac
    13201 ttcttccact accagcttcc ggtcgcctct tgcgtcatta tctcggtggc gatctcccac
    13261 tttttcgtgc aacgcgcttt tgacaaaaaa gataaaaata tcaatcacga acaggcagag
    13321 caaaaagctc tcgataatgt cccgccgctc tattacgcca ttttacctgt gatgccgtta
    13381 atcctgatgc tcggctcgct gttcctcgcc cacgtcgggc tgatgcagtc agaactgcat
    13441 ctggtggtgg tgatgttact gagtttgacg gtgacgatgt ttgttgagtt cttccgcaag
    13501 cataacttgc gcgaaacaat ggacgatgtg caggcgtttt ttgacggcat gggtacgcag
    13561 tttgccaacg tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg
    13621 attggcactg tcgatgcggt tatcaggggg gcggagcatt ctggtctggg cggtattggc
    13681 gtgatgatta ttatggcgct ggtcattgcc atttgtgcca ttgtgatggg ctctggcaat
    13741 gcgccgttta tgtcatttgc cagtcttatt ccgaatatcg cagccggact acatgtacca
    13801 gcggttgtaa tgattatgcc gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg
    13861 attactgcgg tggtagtcgt tacgtcagga attgcaggcg tttcgccttt tgcggtggtg
    13921 aagcggacag cgatccccat ggcagtcggt ttcgtggtga atatgattgc cacaatcacg
    13981 ctattttatt aagtcattaa aaagacaaaa caggccgcct gggcctgttt tgtattactt
    14041 cacaacgcgt aatgccggtc gaccaccgcg tggtggctgc ggaggttcat cgtcaggatg
    14101 agtgtcatca tcgtgatctg gcttgtcgcc atcaataacc gacataacgg tttcgttgtc
    14161 tgccgatgtc tcttcatcat tcatgatgct ggtatcttca tcgtaggcag cttcaggctc
    14221 aaacatcgtg cctgcgccat tttcacgggc gtagatagcc agcacggcag ccagcggcac
    14281 agaaacctga cgcggaatgc caccaaagcg cgcgttaaag cgcacctcat cattcgccag
    14341 ttccagattg ccgacagcac gcggcgcaat gttgagtacg atttgcccgt cacgcgcata
    14401 ttccatagga acctgcacgc cagggagcgt cacatccacc accaggtgcg gcgtgagctg
    14461 gttatccagc aaccactcat agaatgcacg cagcagatag ggacgacgtg gtgttagctg
    14521 tgacaaatcc atacagatta actccggccc agacgcattt cacgttctgc ttcagttaaa
    14581 gaagcaagga aagagtcacg ctcaaagacg cgggtcatat agcctttcag ctctttcgca
    14641 cccgggccgc tgaactcgat gcccagttgc ggcagacgcc acagcagcgg agcaaggtag
    14701 caatcgacca ggctgaactc atcgctcagg aagtacggct tctgaccgaa gaccggcgca
    14761 atcgccagca gttcttcgcg cagttgctta cgtgcggcat ctgcttcaga agctgaaccg
    14821 ttgatgatgg tgttcatcag cgtgtaccag tctttttcga tgcgatgcat gtacagacgg
    14881 ctttcaccgc gagctaccgg gtaaacaggc atcagtggcg gatgcgggaa acgctcatcc
    14941 agatattcca taatgatgcg agattcccac agggtcagct cacgatccac cagggtcgga
    15001 acgctctgat tcgggttgag gtcaatcaga tcctgaggcg gattgtcctt ttccacgtgt
    15061 tcgatctcga aacttacacc tttctcagcc agcacaatgc ggacctgatg gctatagatg
    15121 tcagtaggac cggaaaacag cgtcattacc gaacgtttgt tggcagcgac agccatgaaa
    15181 acctccaggt atagtcagaa tttttactgc taccagccac caggtggcca gtcagaagtt
    15241 gtgttaccca gtaaggaacg actctctttg ttcgaaaatc aaacaaaaaa tgagcaatac
    15301 ccgacatttg ggcagaaaat tggatgatag tttaccagat tttgcgacct ttgtggtgag
    15361 tcgatgccgg aaatggggaa aaagagatgc gctttagtct gaaatagttg acttagtccc
    15421 ttattggcga tgtggttttt gttttgcctg tctgtcagac ggcagcaaaa agcaactttc
    15481 cattttttac gctgattcag aatttaacca taaaaaaacc cgccgaagcg ggttttttcg
    15541 caaattgttt tctgccggag cagaagccaa ttaacgtttg gagaactgcg gacgacgacg
    15601 tgctttacgc agaccgactt tcttacgttc aacctgacga gcgtcacgag taacgaagcc
    15661 agctttacgc agttcagaac gcagggactc gtcgtattcc atcagagcgc gggtgatacc
    15721 gtgacggatc gcaccagcct gaccagagat accaccacct ttaacggtga tgtacaggtc
    15781 cagtttctca accatgtcga ccagttccag cggctgacga actaccatgc gggcagtttc
    15841 acgaccgaag tactgttcca gagaacgttg gttgattacg attttaccgt tgcccggttt
    15901 gatgaaaacg cgagctgcgg aacttttgcg gcgaccagtg ccgtagtatt gattttcagc
    15961 cattgcctat aatcccgatt agatgtcaag aacttgcggt tgctgtgccg cgtggttgtg
    16021 ctcgttaccc gcgtaaactt tcagtttacg gaacatagca cgacccagcg ggccttttgg
    16081 caacatgcct ttaaccgcga tttcaatcac acgctcagga cggcgagcaa tcatctcttc
    16141 aaaggtcgct tgtttgatac caccgatgtg gccggtgtgg tgatagtaca ctttgtcagt
    16201 acgcttgttg ccggttacag caactttgtc agcgttcaga acgatgatat aatcaccggt
    16261 atctacgtgc ggagtgtatt ccgctttgtg cttaccgcgc aggcgacgag ccagttcagt
    16321 agccagacgg cccagagttt taccggtcgc gtcaacaaca taccagtcgc gttttacggt
    16381 ttctggttta gctgtaaaag ttttcattaa aagcttaccc aataaatagt tacacgttgg
    16441 tgaacaccca aacgtcttca attgttgagg ttcacacgac aaagtccggc aaacctaccc
    16501 cttcgaatag cctatgccag cacacaaaaa gttttgggaa aaaaactttc ttgtaacgtg
    16561 gggtcgcagg attatagaga agtcggggtc aaagatcgac ccctttttgt gatttgtggc
    16621 aggttttaac ccgccaaatg ctcgcgcttc agatactctt cgctttgcat ctcttgcaga
    16681 cgtgacaggc aacgctggaa ctcaaacttc agccgctcac cctgataaat ttcatacagc
    16741 ggcacttctg cactcaccac taatttgaca tggcgctcgt aaaactcatc caccagcgca
    16801 ataaagcgcc gcgcttcgct ctccatcaac cgcgtcataa ctggtacatc aaacaacatg
    16861 accgtatgaa agaggcgtga gagcgcaata tagtcatgct gactgcgggc gtcgacgcac
    16921 agcgtagtaa aagaaaccgc cagcgtctgg ttctcgacgc ccattgtcgc caatggccga
    16981 tggttgattt ctaacgtcgg tgaattttct cgtttcgccc ccgccagagc caaccatagt
    17041 ttatccattt gcgcccggat ttcatcgttg agtggcgaaa gccacagatg cgcctgagtg
    17101 agtgtacgca gtcgataatc aacaccagcg tccacgttca ttacatcaca atgctgttta
    17161 atggcatcga ttgcaggcag aaaacgcgca cgttgcaggc catttcgata aagttcatcc
    17221 ggcggaatat ttgacgtcgc taccagggta ataccgcggg cgaacagggc tttcatcaga
    17281 ccgccaagta gcatggcgtc ggtaatatca gaaacaaaaa attcgtcaaa acagagcacg
    17341 tcagtttcgg ctttaaagcg atcggcaata atttccagcg gatcggtctg cccctgtaag
    17401 gcagttagct cttcgtgcac ccgcagcata aaacggtgaa agtgcaggcg ctgtttccgc
    17461 tctcccggca ggctttgata gaaaaggtcc atcagccagg ttttcccgcg tcctacaccg
    17521 ccccacatat ataagccacg cactggcgta tgctttgtgt cttcgcgttt accccacagc
    17581 ttaccgaccc gcgccattag cccactcgtc ctgggggctg gtggcgtgct attgatgagt
    17641 tcctgataaa taatttccag gcggctgacg gcctcttttt gaacgtcgtc gggttgatgg
    17701 ctgccttcat tgagcgcctt caggtattgc gatgttgggg taacgctttg catgatctta
    17761 ttgttattcc ttgaataatc ggtgcgccgt tgttcacggt tgacgaaaaa aaggccgttc
    17821 tacactacgc gatatgcagt cgggattcca cttctgtgga attaacggtt atagtggcat
    17881 aatcagccgc aggcatggag cctgaagcca acaccctacg gaaacaaaag acaacgggag
    17941 atgttcatga cctgggaata tgcgctaatt gggttagtcg tcggcatcat tattggtgct
    18001 gtggccatgc gttttggtaa tcgtaaacta cgccagcaac aggcgttgca gtacgaactg
    18061 gaaaagaata aagctgaact ggacgagtat cgcgaagagc tggttagcca ctttgcccgc
    18121 agcgcggaat tactggatac catggcgcac gactatcgcc agctgtatca gcacatggcg
    18181 aaaagctcca gcagcctgct gccggaactg tctgctgaag caaacccgtt ccgtaatcgt
    18241 ctggcagagt ctgaagccag caacgatcag gcaccggtgc agatgcctcg cgactattct
    18301 gaaggcgcat ccggcctgct gcgtactggc gcgaagcgcg actaatttat ttttcgggcg
    18361 cagccattgc gccctcctct tctctccctc cccgactatc atttaatctg gtgtctcatt
    18421 gttagccgtc tgaaaattca ataacatcaa actgttttga atctcttttc ttatcattca
    18481 ggtacgagag caggaataat gaaaaaacaa acccagctgt tgagtgcatt agcgttaagt
    18541 gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt cgattccagg ccaggttgcc
    18601 gatcaggccc ccctccccag tctggctcca atgctggaaa aagtgcttcc ggcagtggtg
    18661 agcgtacggg tggaaggaac ggccagtcag ggacagaaaa tcccggaaga attcaaaaag
    18721 ttttttggtg atgatttacc ggatcaacct gcacaaccct tcgaaggttt aggctccggt
    18781 gtcatcatca acgccagtaa aggctatgtg ctgaccaaca accatgtgat taatcaggca
    18841 cagaaaatca gtattcagct caatgatggg cgcgagtttg atgcaaaact gattggtagc
    18901 gatgaccaga gcgatatagc cctgttgcaa attcaaaacc cgagcaaatt aacgcaaatc
    18961 gctattgccg actccgataa attgcgcgtc ggtgattttg ccgtggcggt cggtaaccca
    19021 tttggccttg ggcaaaccgc cacctctggc attgtttccg cattaggccg cagcgggttg
    19081 aatcttgaag gtctggaaaa ctttatccag acagatgctt ccattaaccg cggtaactcc
    19141 ggcggtgcac tgttaaacct taacggtgag ttaattggca tcaacactgc aatccttgcg
    19201 cctggcggcg ggagcgtcgg gattggattt gccatcccca gtaatatggc gcgaacactg
    19261 gcgcagcagc ttatcgactt tggtgaaatc aaacgcggtt tgttaggcat caaaggcacc
    19321 gagatgagtg ccgatatcgc caaagccttc aaccttgacg tgcagcgtgg cgcgtttgtc
    19381 agcgaagtgt tgccaggttc tggctcagcg aaagcgggcg tcaaagcggg cgatattatt
    19441 accagcctca acggcaaacc gctgaatagc tttgctgagt tgcgctctcg tatcgcgacc
    19501 accgagccgg gcacaaaagt gaaacttggc ctgctgcgta acggcaaacc actggaagta
    19561 gaagtgacgc tcgataccag cacctcttcg tcggccagcg ctgaaatgat cacgccagcg
    19621 ctggaaggtg caacgttgag cgatggtcag ctaaaagatg gcggcaaagg tattaaaatc
    19681 gatgaagttg tcaaaggaag cccagctgct caggcaggct tgcaaaaaga cgatgtgatc
    19741 attggcgtca accgcgatcg ggtgaactcg attgctgaaa tgcgtaaagt gctggcggca
    19801 aaaccggcca tcatcgccct gcaaattgta cgcggcaatg aaagcatcta tctgctgatg
    19861 cgttaatgtc gtaaaccggg catcaggctg acgtgtgatg tccggttaac tcgtggtatg
    19921 ctgctgccgt tccctttttt aatgacgcct ccatcatgtt tgtgaagctc ttacgttccg
    19981 ttgcgattgg attaattgtc ggcgctattc tgctggttgc catgccttcg ctgcgcagcc
    20041 ttaacccgct ttccactccg caatttgaca gtaccgatga gacgcctgcc agctataatc
    20101 tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta caaccgtggt ttgaacacca
    20161 actctcacaa ccagcttgag atccgcaccc tgggatccgg tgtaatcatg gatcaacgcg
    20221 gttatatcat caccaataaa cacgtcatca acgacgccga tcagatcatc gtcgccttac
    20281 aggatggacg tgtatttgaa gcattgctgg tgggatctga ctctctaacc gatctggcgg
    20341 tacttaaaat taatgccact ggcggtttac ctaccattcc aatcaatgca cgtcgcgtac
    20401 cgcacattgg cgacgtggta ctggcgatcg gtaacccgta caacctcggg cagaccatta
    20461 cccaggggat tattagtgcc acgggtcgaa ttggtctgaa cccgaccggg cggcaaaact
    20521 tcctgcaaac cgatgcttcc attaaccacg gtaactctgg cggcgcgctg gtgaactcgc
    20581 tgggcgaact gatgggcatt aacacgctgt cgtttgataa gagtaacgat ggcgaaacgc
    20641 cggaaggtat cggctttgcg attcctttcc agttagcaac caaaattatg gataagctga
    20701 tccgcgatgg tcgcgtgatc cgcggctaca ttggcattgg cggacgtgag atcgcaccac
    20761 tgcacgcaca gggcggtggt atagatcaac tgcaagggat cgtggttaat gaagtgtcac
    20821 ctgacggccc ggcggcgaat gcgggtattc aggtcaacga tctgattatt tcggtggata
    20881 acaaaccggc catctctgct ctggagacga tggatcaggt ggcagaaatt cgccctggtt
    20941 cggtgatccc ggttgtagtg atgcgtgatg ataagcagtt aacgctgcag gtcaccattc
    21001 aggaatatcc ggcaaccaat taagtcgagt gctcaaaaca aaaaaccgga gtctgtgctc
    21061 cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc agggcgatat
    21121 ctttcttgag cgtatccagc ataccttcca gcgcgttctg ttcaaatgcg ctcagggtac
    21181 cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga gagaagaaac
    21241 gggcgtactg accgtcgcct tcaacgtagg cacattcgac aacgccttgt tcgccctgca
    21301 gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac agggttgcag
    21361 acccgccacc ggccttcgct tcaaccactt cagtacccgc gttctggatg cgtttggtca
    21421 gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc agcggcagaa
    21481 tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcctggc tgtttgcctt
    21541 tcagttccgc aacaaaggtg ttggaacgaa tgatatccag cgtggtaacg ccgaacagtt
    21601 tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca actgtggtgt
    21661 taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca acttgctgta
    21721 ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc atacccggtt
    21781 tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc ggagtcgcat
    21841 cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga tcgacggcca
    21901 caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa ggcagttggg
    21961 tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg actgcgactt
    22021 tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct gccgcgacct
    22081 taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt cacgcaagtt
    22141 tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt aacagtcgct
    22201 tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt cagccaaaac
    22261 aacatcaatt tgataacaat taatttactt ttaagcagaa tttgcatgcc gtgacgcagg
    22321 catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt atttcagcct
    22381 tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg ttgtatcaac
    22441 caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt taaagcattt
    22501 aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc cgcgttgcag
    22561 gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac caagtttggt
    22621 gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc tgaactgggt
    22681 gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta caacgatgca
    22741 gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct gctggactca
    22801 ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacacaat ctttaccacc
    22861 cctgctaacg gtttcacagt caaagacctg tacgaagcga ttttagagct gttcgaccag
    22921 gagctttaat ctctgccccg tcgtttctga cggcggggaa aatgttgctt atccctctca
    22981 accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttactgc gacatgtcat
    23041 tcacacaatg aatacataag gtaaaaaaag cacattatgc aaaattcatt atctaattga
    23101 aaaaactaga attaacgata aataaccgta tttttaattc ttttttgtta ttaaaattca
    23161 catttttaac acttagtatc aactgaaaca gttagcgcgg tattaattag ctcaataatt
    23221 agtgtatact tgattttgtg atatgggtca cgaaacaaag gcccagctaa aagattatgt
    23281 cgagttaaaa atcatgaaaa tcaaaaccac tgttgctgca ttaagcgtac tttctgttct
    23341 ctctttcggt gcattcgctg ccgactccat tgatgctgca caagcacaaa atcgtgaagc
    23401 aatcgggacc gtatccgtaa gtggtgtggc gtcttcgcca atggatatgc gtgaaatgct
    23461 gaacaaaaaa gcggaagaga aaggcgcaac ggcctaccag attactgaag ctcgtagcgg
    23521 tgacacctgg cacgctacgg ctgaactgta caaataaacc ctcatcgtct tgtccgacga
    23581 tattgccccc ggttcggggg cttttttttg cgctaatgac gaacattaaa acgcaaatgc
    23641 ccttccagct cttcctctgc ctcatcaaac agcaatatta acgcgccaaa acggcgacgc
    23701 gttttctctc ccagatgaac aaattcaatc tcaagtggta gcggcaggac atcgttcatt
    23761 aacacatccc atagtgagtc gagatcgcgt accttatctt tcgccagacc aaaggtttgg
    23821 ctaaagtcac gataaaaatc ctcctgactc tcaatctcat caaaatcaaa ggtataaata
    23881 ttcatctgtt gccaccgtca cgtttcgcgg cgagttctgc cgtgcatcgc taagtatagc
    23941 catgaaaaaa ccgacgcttt tggcgtcggt tttgacttaa ctatcggtca acgcatgttg
    24001 atacttatgg agcatccccg ccagccgatg aaccggttcc gtcacctgcg gtggagcttg
    24061 ccagatgcgt agcttttcct ggtagatttc cagttcttcc agcaactgac caaagtaccg
    24121 acgacgttta tcatcgctac gggcagatat cacatggtct gctgtgcggc gcatttgtcg
    24181 gtgaaacgcc gataaatcct cgttaaccgg gatcggcgca tcacgcaggc gctggtgggc
    24241 gataatcatc gtcagcgcca ggcgaaattt cggcaaatcc cctgggaact tattcatcag
    24301 caaaaacagc tgctgataaa gtgccgggag gtggttctct ttacgacgtg ccacattggt
    24361 agtcatcgcg gaaacagcgg cagaaacaaa ctgattaagc agtacacgtc cagtcctgtc
    24421 gcgcgattta tcccgcacca gcaaaataac ggtgaacgcg agcacacagc cgacgatttg
    24481 ccctaatgcg ctgtcgagaa actgactgaa atggaaagtc atcgggttat ccagcacgat
    24541 aatatttatg gtgctggcca gtgcccccat cgagcccagt cgccgtttct gtacttctat
    24601 accgaggaag aatcccagca ctgccaggct aatacacagc agcaacatgc tctgttgggt
    24661 attagggata atcaccaaaa agtagagcag ccctaacggc agcgcggcca gcgtcccgta
    24721 gataaagtcg atcgccacca tgcgtggatt cggcaaacgc attgccagtg acgtcacgac
    24781 cgcaatcatc accattgcac cactaccgga agtccagccc gtccacagcc agaaaagcgt
    24841 tcccagaatg caggaaagtg tggttcgcca gaagttaacc attgcatgat gacgttcggc
    24901 tgactctact tttacttccg gttcgccttg caggatctct tcttcggtgg cgttgatttt
    24961 tgtgttactg ataacgccgc gcttgagaag ctgataacgc gttgccgccg cgacccagct
    25021 ataaatggtg acaggcgttt cccgttcccc ggtccaggcg ataactcttc gcaggcgttt
    25081 gagctgcttg tggacgtcct gcgcggtttc taccggtgtg tcaaaaaatt cgcggaaagt
    25141 atcagtgatc aattccgggc gcgtattctg aataagataa gtttcgcagg attgggtaat
    25201 cagcgtcagc gatagcgtat tgatcgcttt taaacgtcga ttggcccgcg cccagcggga
    25261 agattccata ttcaggttgc tgcgcatacc ttgtagcgcc gtggtgcgtc gaaccaggtc
    25321 gccccaggct ttatcgacaa cttcaccatc gccatgcttg atacagagtt gcattaattg
    25381 atattgcgcg accagcaaac tttccagctc tcgatccact tcttgtttga tcgatcgcgg
    25441 agaaaagagc aaatccgcca taattgcaca cacaataccg ataacgatct cgctacaacg
    25501 ttcgacggca aactgcggcg taagcaatgg ttcaggctga atggtgatca caatgatcag
    25561 cgcggtataa ccggccagcc cccacgcata cgagttttct attcgtacca gcgaggatat
    25621 ccaggtacaa aaaccggccc agatacagca caccagaatc atcaataatg gtgcgcggat
    25681 catcgcaatg atgatcacca gtccggcaat acagccaata aatgtgccga tgatgcgcaa
    25741 aaagccacga tagcgaatag cgccagaata cggttcacct cccgcagcaa aggccggacc
    25801 ggcagcaaca atcgccgctg tcagtaccgc ccagcgtggc gtttccagct ggaagtgaaa
    25861 gccaacaaac agcgccagta caatggcggt cgccagtttt accgcaaagc gaatatgttg
    25921 gttagcaatg gagaaaatac ccatcgtgat taaccaaact cacgcaggcg atgggccatt
    25981 ttacggaaga acgaatcctg gctttcgtcg cgatcttgtt tgccagtgac caccactgta
    26041 gcagtggtgc ccgcaggcca gatgttctct tgctggttgt cgagacgaat acgaaccgga
    26101 acacgttgcg ccaaacgcac ccattcaagg ttagagtcga tggtcgccat ccctttgtcg
    26161 tcacgcgtgc tgctggcgtt ggtgacccca gcggcaacac tatcaacagt ccctttcagc
    26221 actttgttac tgccaagcgg cgtgatctct gcacgatacc cagggcgcac cccttccagc
    26281 ttagtttctt ccatataggc cagtacatag aaagagttct gtttcaccag cgcaaccgcc
    26341 gttgatccac gagtaataaa ctcaccggta tagacgttga ggttggtcac ccagccatcc
    26401 gctggcgcgc ggatcaccgt gcgttcaaga tccagttttg ccagatcgcg ggtcgcctgc
    26461 gctttcgcta actgatgcag aaccgtttgt agtacgttgt tggcctggtc gatctcttcg
    26521 cgagacatcg cctgcacacc gagacgatta cgacgtccgg cctcctggcg tttctcctgt
    26581 gccagtacct gataataagc aacatcggct tgcgcttcct caagcgcctt ttgatagcgc
    26641 ggctggtcga tggtgaacag tacctgtcct tttttcacca gctggttatc atgaacattc
    26701 acctgggtaa tgagtccaga aacgtccggc gcgatcgcaa cgacatcagc gctaaagcgc
    26761 gcgtcacgcg tccacgggga ttcggtgtaa tagacccagg cattaaaaat tgcgatgaag
    26821 gccagaatga ctaatacgac cgtgatggcc gtacgggaga attttcttat tagtgttttc
    26881 acttcaacct caaacgaaca gtcgcgatat caaataaaac aagcagcaat agagcgcggt
    26941 gttgaacaac gccggatgcc agacaaagtc gtagatacct gttggcacaa gtacccggcg
    27001 caccagccag aaaatcgcca gtgataaaag caattcaaaa aatatcggtg ggaaggacag
    27061 cccaaacacc acgataacgg gaaacagact catgtcgacc ttggttgtat agagagagca
    27121 ggcgttatta ttttcagcat ctgtcgccgc agagaagggc atggaaagcc gggcgagagc
    27181 aacattgctg tagattgata tttaatatat tagcgtaact gttatgctgt tatctatatt
    27241 atgtgatcta aatcactttt aagtcagagt gaataatgga acgactaaaa cgcatgtcgg
    27301 tgtttgccaa agtagttgaa tttggctctt ttaccgccgc cgccagacag ctacagatga
    27361 gcgtttcgtc catcagtcag acggtgtcaa aactggaaga tgagttgcag gtaaagctgt
    27421 taaaccgtag tacacgcagc attggtctga ctgaagccgg cagaatttac taccagggct
    27481 gccgtcggat gctccatgaa gtgcaggatg ttcatgagca actgtatgcc ttcaataaca
    27541 cccccatcgg gacgctgcgc attggctgtt cttcaactat ggcacaaaat gttctcgccg
    27601 ggctgacagc aaaaatgctg aaagaatacc caggtttgag cgtcaatctg gttaccggaa
    27661 ttcctgcccc cgacctgatt gccgatggtc tggatgtggt aatccgcgtc ggcgcgttgc
    27721 aggattccag cctgttttcc cgccgtctgg gcgcgatgcc gatggtggtg tgcgccgcga
    27781 aaagctatct cacacaatac ggcataccgg aaaaacccgc cgatttgagt agtcattcat
    27841 ggcttgaata cagcgtgcgg cccgacaatg aatttgaact gatcgcaccg gaagggatct
    27901 cgactcgcct gatcccacaa ggaagatttg tgactaatga tccgatgacg ctggtgcgct
    27961 ggctgacggc gggtgccggg atcgcctacg tgccgctgat gtgggtgatc aacgagatca
    28021 atcgtgggga gctggagatc ctgctgccgc gttaccagtc agatccacgc ccggtttatg
    28081 cgttgtatac cgaaaaagat aaactgccgc tgaaggtaca ggtcgtgatc aactcgctga
    28141 cggattattt tgttgaggtc ggtaaattgt ttcaggagat gcatgggcgc gggaaagaga
    28201 agtaattcat attgtactgt tacgttgttc aaacctgtgc caacgggttc ccctcaccct
    28261 aaccctctcc ccaaaggggc gaggggaccg ttcgtgcacg tagaaagatt aattatcctt
    28321 ctgaaaatag tgaaattatt acgcagtacc gccaacagtc aggttatcga ctttcaacgt
    28381 tggctggccc acgccaaccg gcaaactttg cccttcttta ccgcagacac ccacgccgtt
    28441 atccagtttc aggtcgttgc caaccatcga aatctgctgc atggtttcga taccggaacc
    28501 aatcaacgtt gcgcctttca ccggcttcgt tactttaccg ttttcaatca gatatgcttc
    28561 tgaagtggag aaaacgaatt tgccggaggt gatatccacc tgaccgccac caaagttcgg
    28621 tgcatagata ccgtactcaa cggattcaat aatttcctgc ggggtcgatt tacccggcag
    28681 catataggtg ttggtcatac gcggcatggg cagatgggcg taggattcac ggcgaccgtt
    28741 gccagtcggc gtcatcccca tcaaacgcgc gttgagttta tcctgcatgt agcctttcag
    28801 aatgccgttc tcaatcagca cgttgtactg gcctggcgta ccttcgtcat caatcgccac
    28861 cgaacctcgg cgatcgacca tcgtgccatc atcaaccacg gtacacagtt ctgaagccac
    28921 cagctccccg acctgtccac taaatactga agtgccacgg cggttgaagt cgccttccag
    28981 accgtgacca accgcttcat gcaacagcac gcccggccaa cctgcgccaa gtactaccgg
    29041 catggtgccc gctggtgcag caacggcaga aagattgacc agcgccatac gcactgcttc
    29101 ttttgcccat gcatccgcac ggacttcgcc gtcgagatcg gcaaggaaga attcataacc
    29161 aaaacgaccg ccgccgccac tggcaccgcg ttcgcgtttg ccatcttctt cgacgagaac
    29221 gctcacggaa agacgcacca gcggacggac atccgccgct agcgtgccgt cggtggccgc
    29281 aaccaaaatt aattcataga caccactgag gctggcagtc acttcctgta cgcgcttgtc
    29341 cgcttcgcgg gcaaccttat cgacgcgacg caggatatcc agcttctctt cacggctcat
    29401 gctttgcagc ggatctaccg aggtatacaa cgggctatgc tctaccgcgc ccagcgtctg
    29461 tactttacca tcaccactat cacggacgat ggtgcgcgcc gcttgcgcac tctgttccag
    29521 cgccagcagg ctgatttggt cagcgtaagc aaatccggtt ttttcaccgc tgattgcacg
    29581 cacaccaacg ccctgatcga tgttgtaaga accatcttta ataatgcggt cttctaaaac
    29641 ccaggattcg tgatagctcg actgaaaata gagatcgcca taatcaaggc gacgttcggc
    29701 cagttgaccg aggatcgcga acaagtcctg atgtttcagg ccgttcgccg ctagcaattg
    29761 ttcacttacc aggttaagac tcatcgtttt tgctactcgt tagttactgc agtagaggat
    29821 tttttacggc tgccggatgc ggcgtgaacg ccctatccag cctacggtta tgttccggtt
    29881 tgtaggcctg ataagacgca cagcgtcgca tcaggcaacg gctgtcggat gcggcgtaaa
    29941 cgccttatcc gacctacggt tatgttccgt ttgtaggcct gataagacgc acagcgtcgc
    30001 atcaggcaac ggctgccgga tgcggcgtaa acgccttatc cgacctacgg ttatgttccg
    30061 tttgtaggcc tgataagacg cacagcgtcg catcaggcaa cggctgccgg atgcggcgtg
    30121 aacgccctat ccgacctacg gttatgttct ggctcgttgg cctttggcaa cgattatcct
    30181 atgagattgg ggcaattacg cgccctcgtc aaatcattgc gctttttctt tacgcggttg
    30241 gcgcaacact tcgttgattt gcggatcgtc cagcggaccc gaaatgtgat agcgcaaaat
    30301 ggagactttg ctccacagcg gccccagcac tttactggcg gcaaacactg ccgcgccaac
    30361 aatggggtta accgcaaaag ccgcagccac gcccaccgtc gcagaaatct ctggtgcgac
    30421 aaccgcttcc atattcaggt cgcgacgtac cagatttacc gaccctttca tggcgatatc
    30481 cgcctccagg ccatccacca gcgtgtcgtc ggtgtgcata acgccgtctt taatccacgc
    30541 ggtgctgcga atggagtcaa aatagaaccc ttcgccaaaa gtgtctctga aatcaaaacg
    30601 cagcttacgc atcagggcat ctacgctcaa taagcgcagc aattgcccgg catgtccggt
    30661 attgatttcg gtaatttcgc ctttacccag ttgagtatga atgatgccat tcaacgtcgc
    30721 ttcatctggc tgccagggtg ctttgcgcca gtgtaaatcg taatccacat taaatgacga
    30781 ctggcgtatg ggcgtcgtga caccaaaaaa ttctgcggcg gcatcaattt tctgcccgcg
    30841 cagttttcct ttcagcgagg tacgttcatt ccccggatta ttaacccatt caccatcggc
    30901 agtaagccgc gagaaaccag tatcaatcag tccattggtc agcgttaacg tatcgccaga
    30961 aatggtgata tcactgtcaa tgcgaccgaa tttttgcccc cagaaccagc actctgtgca
    31021 tcgtatttgg gcgtccggcc agccacggaa gttaatgcgc tccgttgtcg ggaaaggtga
    31081 tgacggcgtt gaatcaccac gagttttcgc cacgctcggg ttgtaataaa gatatttgat
    31141 attcgccagc cacggcgcgt tattacgcat cgctagcgtg gcgttgattt cacgcccttg
    31201 cgcttcaacc agggtgccat ttgccgtcgg ttgcgaaaca atactcaggt tattccattg
    31261 ctgatttccc agtgacaaca taggcgtacg taacgttatg tgttgtggga aactcgctgc
    31321 accaccgaca ctctccgcag cgcctttctg gaacagggcc agccactcgg caccattcat
    31381 cggcggcata ttgagttcaa cgccactttg ttccggcaac ggcgggagcg ttttactgtc
    31441 tgccgcccaa atagcacgat cgagcgtcag cttttgaccg agcaaccagc ggctattgaa
    31501 atgattatcc gcaccagcct gtccggttaa ttcaaagctg ttgagattgc catcaacctt
    31561 aacgtttacc gctagtggtt cacccgcagg tttggctaac ggtgaaggta agtgactgct
    31621 cacattcttt agatcgccgt tcagctctat gttataggtc gcaccagcat gataaggcag
    31681 atcaatgccc actttaccat cccacgccac gctgccactc aatgcttcgt tcaccgcttc
    31741 aggcagaacg ccggttttcg ccggttgcca gttaccgttg aggtttaccg ctacctggta
    31801 ggcttttgcc ccttctttgg tggaaaaatc cacgttcaac ggctgattaa accagcttgc
    31861 tgtcagtggt tcactttgca gatcgctatt gataaagctg aatttaccgc tcaaattttt
    31921 cagggtgctg tcgagtggtt tgataaacag actgttatta cgcagcgtca cttcaccttt
    31981 cgcggttacc agttcgccgt tcagcgggat atcaagatgt aagcgagcat tcacatcgcc
    32041 gtcgagctgg agttcttgca gggtcgcccc cagagaatct ttcagcggtg tctcatcaaa
    32101 gtaagggcca acggctttac ccggaccttt aatgtcagcg tcaatcagca gtttttcttt
    32161 cgagtagtca gggatcactg cggtgagatt actcgcgcgc acgccgccca gattaacgcc
    32221 atcggttttc atccataaac cgtcgttaat aaagtccagt tcaatatcaa ggttagttaa
    32281 tgcaggccag tccggctgga aggcaaactt cgcgttgcgc agcggcacca gcacttcaaa
    32341 ctgaccttcg ttgtgtttat aggggaagag ttgcggattg ccaccataaa ccagcgtcgc
    32401 gttatccgct tcaccgccct gaatcgcgcc acttaagtaa tcaaccaggt ctttacccat
    32461 caagttttcc gggaaatagc gccaggcttg tgaaccatca tcggtactga tgccagccag
    32521 aatacccagc cagggttcat cgttagcagg ttgcaggtaa cgaaaaccgc cgcgcgcatg
    32581 gacggctttg gctttaacgt caatatcact cccatccagc tggaaacctt tgtcattgtt
    32641 cagccagctt atagttgcct ggccgtcggc gatttccagt ggcgcacgga ataccgtttc
    32701 gtaaggcatc tttgcctgct tcatcgacgc ggtaagcaaa ccattttcaa cgctgccgga
    32761 aagcgtcccg gagaagtgtt ccgcacccgg taataatttc cattgcttcc aggccagatc
    32821 gctccacgat gcctgaaaac gggtcttgtc tgccgcctga agcgggatat ccagcgccag
    32881 agtgttaatc ttgccgctcg gttgtgtgga gcgccaaaca tcacccagtg caggtgaaag
    32941 tttcgcggcc agcgggcgta tgccctccag gcctgccagc tccagattac tggcgcgaat
    33001 ccggagttcg tcactgcgtt tattgtcttt gccgccaacg tcctgttccg gtatccaggc
    33061 tagcgtcaat gctccgctcg gccagggttt gccgtccatc gtgatccgtg tatcgggaat
    33121 agagaactgc cagcccggat tttcacgcgt aatatgcgcg gtcagattat tcaccgacag
    33181 cgtatgcgtt tgcttctcgc ccaaccagct ggcaccgccc tgtttcagcc agacgtcgcc
    33241 accggttaca tcgcctttat cgatcgtcat ccagccttca agggagaact gcgccgtttc
    33301 cagcgcaata ttgtcctgca tccatttacc gagccacggc ttcaggtcga tgtcatccgc
    33361 ctggagccag acgcgaccat tgcttaacaa gccctcatca tcgcgcaaat ccatgcgcac
    33421 ctgcattacg ccgtgctgtc cggtaaggct ggagaggctt accagacctt ccgcacggtg
    33481 tcgacgtgga tcgttcagcc aggtgagttg tgggatcgcc agctcggcgc gctgaccgga
    33541 tggcgtcagg aaactgactt cactgtcgcg gagatcgaaa tggtcaaatt gacgaagaaa
    33601 cagatcgctg atgtggctgg cttccagact gtcatcgcta ccaccgctgg tgataggagt
    33661 gttggtgcga aagcgcagct gccagaaagt gagatcgcga aactgccagc gcatatgtaa
    33721 caggctctgc cagacatcca gcgccagagt aacgcgttta accgaaaatt cgccgccatc
    33781 ttttagttct gcacggatgt cgtgtgcttc aagcgtcggg ccaaaattct gccagctggc
    33841 tgagagctga ctggcttcaa ccggcatgcc agtcgcggat tctattttgt tgaggatttc
    33901 cggacgccag gcgtcaagat gcggtaaagc aatacgcagg ccgctaacca gcagggcagc
    33961 gatcacaacg agcgcggctc cagtaagcag taaaatcccc ggcaatcgcc tcacccgtca
    34021 ctccttgtct gctaaaaatg tgactcaaaa accctttgcc ggatggcggc ccagcatctg
    34081 tttacatcat tacgacgtca aactgctcct ggttatagag cggttcaatt tgtactttaa
    34141 cctgtttgcc aacgaaaatt tccacttccg ccagcgagtg tgactcttcg cctttcaagg
    34201 cttcggctac tgccggagaa gcatagacca ggaaacggtc ggaatcgtaa gcatggtgga
    34261 cacgaacaat ctcgcgcatg atttcatagc ataccgtttc cacggttttc accgttccgc
    34321 gaccgtggca ggttgggcat tcgttacaca gtacgtgctc aatgctttcg cgggtgcgtt
    34381 tacgcgtcat ctccaccagc cccagcgccg aaaaaccatt aacgctggtt ttcacccggt
    34441 ctttgctcaa cgcctgctcc agcgagtgca gcacgcggcg gcggtgatct tcattattca
    34501 tatcgatgaa atcaataatg ataatcccgc ccagattacg caaccgtaac tggcgagcga
    34561 tagcctgcgt cgcttcaata ttggtattga aaatggtgtc gtccagattg cgatgaccga
    34621 caaacgctcc ggtattgatg tccacggtgg tcatcgcttc ggtctggtcg ataatcagat
    34681 aaccaccgga tttcagttct actttgcgtt ccagcgctcg ctggatttcg ttttcgacat
    34741 caaagagatc gaaaatcggc tggcgtccgg tgtaatgctc cagcttgctg gtcatctcgg
    34801 gaatgtactc cgaggtaaac tccagcaatg cttcgtaagt caggcgtgag tcaacgcgaa
    34861 tgcggtccag ttcggcgtcg gcgaaatcac gcagaacacg ctgcgccagc gccagttcgc
    34921 cgtacagctg ataacgggtc tgcgggcgtt ttttacgctc cataactttg gtccagacgc
    34981 gtttcagata agcggcatcg gaggccagtt cagcctcgcc aaccccttcc gctgcggtac
    35041 ggatgataaa cccgccctgc tcgtcgcaat actctgcgac cacttttttc aggcgttcac
    35101 gttctgattc gctttcaata cgttgggaaa ccccaacgtg agaagcccct ggcataaaca
    35161 ccagatagcg agaaggcagc gtgatatcgg tggtcaggcg cgcaccttta gtgccaagcg
    35221 gatctttcac cacctgcacc atcagatctt gcccctgacg aaccagttcc gagatgtcgc
    35281 gcaccgtgaa ttgcttttgt tcttcacccg ccacacattc ggtgtgcggc atgatgtcag
    35341 atgcatgaag aaacgcggct ttatccagcc caatatctac aaaagccgcc tgcatacccg
    35401 gaagtacacg acttacacga cccttgtaga tattgcctac tattccgcgt cgcgcctcac
    35461 gttcaatatg aatttcctgc agaataccgc catcaatata cgccactcgc gtttccgaag
    35521 gcgttacgtt tactaacaat tcagccgtca tgtttatccc ttttctcacg cagtgcgtta
    35581 aaattactta ataattcata cgtttcaacc agcggtaagc cgactacggc gtgatagctg
    35641 ccatttatct tcctgacaaa acagccaccc agcccctgaa taccgtatgc acctgcttta
    35701 tctaacggtt caccgctggc gacatagccc gcgatgtctt cgtctgttaa cgttctgaaa
    35761 gtcacatcgg tgaccaccag gcaatcgaga atgtgctggc tgtcagccaa cgccactgcc
    35821 gtcatcacct gatgggtctg acccgataat ttgcgcaaca tctgcgccgc atgctctgcg
    35881 tcgcgcggtt tctccagcac ttctccgttc aggataacga tagtatccgc acccagcacc
    35941 gggagatcct gcgccgtttg cgcgacaccc gcttgtgctt tctcgcgtgc cagacgcaca
    36001 acatactgct gcgcgctctc ctgcggctga cgctgctcct caatgcccgt aacaatacgt
    36061 tcaaaggtca cgccaagttg cgcaagtaac tcctgacgac gcggagaacc ggaagctaaa
    36121 tacagagaag tcatagaaac ctttattgca ctgcaaactg ctgacggact ttacgcatca
    36181 gcaagaaaat ccacggccag agcaccccat tgactacact actccagaac acttccggtc
    36241 tgaaagagac gttaatcact aaaaactctg cccagaaaac aataatatcc accaccagcg
    36301 aaagcaacat gacgactagc gcctgctgcc ataatgcgag gttgcggaaa agctggtatt
    36361 tcagcgccac caggtaagca atgatgctca tcgccaatac gcgtacgcca agcgtcgagc
    36421 cgctgatcag atccagtatg gcacccatca caaaacctgt gcccacattt acgcgatggg
    36481 gcaaggccag gatccaatac aacaagataa gtaacaccca gtttggccgg aaaacaatca
    36541 ggttatccgg ccagggcatg atttgcagca acagcgcaat gaggaaagag agccagatta
    36601 cccagcgtcc ctggctacga tagctcgcca ctattgccct cccggcgcac gcgcaggcgg
    36661 ttgagcacca ctttgcggcg gcgtagccct ttgtggagag cgattagcag cgggctgtgt
    36721 cggcgcagca ggcgcagtag ctgcatttcc tgtcgtcggt tgctgcggag tcggctgagc
    36781 gatccccgtt gccggttcag gtaactttgg ccccatcgcg tctggcgaag gcaatacctg
    36841 cggcatcatc tgcatcagac gttcattagc aacacgatgc acctcttccg gcgtcatcgg
    36901 gttagcgccg ttacgatctg ccccccacag cagcaacaga taacgcaaac gttgcagccc
    36961 tgcagtcgga cgcgcctgga tcacagtata agcgcgctgg gtatcgagtt ttacggaaga
    37021 gacaaccgcg accggatagc cttccgggaa acgaccgccc agaccggagg tcaccaacac
    37081 atcaccaaca cgaatatccg tatttgccgg cagatgctca agctgcaaat catccgtaca
    37141 accgttaccg gctgcaatta cgcggatatc gttgcgcagc acctggattg gcagcgcgtg
    37201 ggtcgcatca caaatcagca gcacgcgact ggtcagttta gcgacggcca ccacctgacc
    37261 aacaacacct ttgtcgctga tgaccggctg gccttcataa acgccattaa cgctaccttt
    37321 atcgataaca acttgatcgc tataaggatc gttaaccgtg gagataacct gagtcaccat
    37381 tttctgctca tcctgacgca gcggggaacc cagcagctcg cgcagacgcg cgttctcctg
    37441 tttgtattgt ccaagcatca gcagttcact gtttttcagc aacagttcct gacgtaacgc
    37501 ccggttttca agttctagtt ggtcacgcga ggccagcgtc tgcgatacgc catccagcaa
    37561 ttcacgagga gcattggaaa caaagtagaa aggactgacg gcggtatcca tataagtacg
    37621 gatttgactg aacgtcccca ggcggctgtc ggcaataata atgccgagcg ccaccagcac
    37681 cgccagaata aggcgaatct gtagcgacgg gccacggcta aaaattggct tcataagtta
    37741 tgcgtattct cgtatcagac caggcagggt aaacagacac ttcccctgcc tgcatccgat
    37801 tactcttcgc tgaacaggtc gccgccgtgc atgtcgatca tttccagcgc tttgccgcca
    37861 ccgcgcgcca cacaggtcag cgggtcttca gcaacaacga ctggaatgcc ggtttcttcc
    37921 attaacaaac ggtcaaggtt acgcagcagt gcgccaccac cggtgagcac catgccgcgc
    37981 tcggagatgt cggaagccag ttccggcggg cactgttcca gtgcaaccat taccgcgctc
    38041 acaataccgg tcagcggttc ctgcagtgct tcgaggattt cattggagtt cagggtaaaa
    38101 ccgcgtggaa caccttctgc caggttacgg ccacgaactt cgatttcacg gacttcatcg
    38161 cccggataag ccgaaccgat ttcgtgcttg atacgttctg cggtggcttc accgatcaga
    38221 gaaccgtaat tacgacgcac atagttgatg atagcttcgt cgaaacggtc accaccaatg
    38281 cgcacagaag aggagtaaac cacaccgttc aaggagataa cagcaacttc agtggtacca
    38341 ccaccgatat caaccaccat agaaccggtc gcttcagaaa ccggcaggcc agcaccaatt
    38401 gcggcagcca tcggttcttc aatcaggaag acttcacggg caccagcgcc ctgcgcggat
    38461 tcacgaattg cgcggcgttc aacctgggtc gcgccaaccg gcacacaaac cagaacgcgc
    38521 gggcttggac gcataaagct gttgctgtgc acttgtttga tgaagtgctg gagcattttt
    38581 tcagtcacga agaagtcggc gataacgccg tctttcattg ggcgaatggc agcaatattg
    38641 cccggcgtac ggcccagcat ctgcttcgcg tcatgaccta ctgcagctac gcttttcggt
    38701 gaaccggcac gatcctgacg aatggccacc acggaaggct cattcaatac gatgccttgt
    38761 ccttttacat aaatgagggt attcgcagta cccaggtcaa tggacaagtc attggaaaac
    38821 atgccacgaa attttttcaa catactaagg gataatcctg aaagctgggg cggaaaagaa
    38881 aatccgctta ctttaccaac cacacgcagc agcgacaagg cgcaaaaatc atctgctacg
    38941 gtgaaaatta gtgcagttcg tttcctttgt tacaaatctc tgcctgagtc cagaaaggct
    39001 taatgcatca gcagcattcc tcgcctgttt gcaaccgcgt aaggtcattc atctgcatat
    39061 gtgctgcaac aatctggcga gcagacaagc acactcccat gagacgcagc gcgcgttatt
    39121 ctacgtgaaa acggattaaa cggcaggtta aaccgagtat ctttgtgaat attttttcac
    39181 gttagtatca agtggctgtg aggacgcgaa aaaatcccct tgcccgcctg taacaccgcg
    39241 ctgaatcagg gtctgccact cgcttcgcga acgcacgccg gtggcgtaaa cctgggtgct
    39301 ggtcccggag caggcttcca ccaggctttg aaccagcagc tggttctccg ttcgcttctc
    39361 aatgtttctg accagccccg gatggagctt gagtaactca acattaagtt ctttgatcca
    39421 actggtactt accagcgtca aaccagcctg gttgacggct acccgtaccc ctaaagcatt
    39481 cactaaacga ataacaggtt gtaaacgact gatatgttga cctacatcgg cctctgcaag
    39541 ttcaataatt atgcgttttc gttgtgattt ttcacattgc attaacgtat cacgcagcca
    39601 acgctgaaaa cgcgggcgaa tcagcgactc aacggtaacc tgaatcgcca gattttcctc
    39661 tggccagtaa cgcaatagtg gaataagacg gctgatttgc agacggtcat actcttccga
    39721 taagccaaac tgcaagacca tcggcatata ctccgccgag ctaacctctt cattaccatc
    39781 gaagatgcgg cacatgagtt cgcgatgatg aacctgacct tcgcgagtaa ccgccggttt
    39841 ttgataaagg cgcgggccgc cgcgactgag catttgctcg ataagcgtac gccagcgaac
    39901 attaccgcgt cctttttcag gcaacgagtc atcgtaaata gcccagctat tgccgccctg
    39961 caatcccgca ttacgcatgg cagactctgc atgttccatt acctgctcgg tatcctgacc
    40021 actacgccag gcgcagatac caatgtggat catatcgtcg cgatcgagca ttttattgtt
    40081 cggcaaggta tcaacggctt tgattaactg accggcgatg ctctctgcct cttttaacgt
    40141 ccggtgcggt aacagcgcag caaaatcact gcggtggtaa cgcgccagca gtgcgccagg
    40201 gtagcgcatc ataaatgtcg acagcagatt cgtcagagtg aagaactgtt cttcaacctg
    40261 gctgtgcccc caggtatcgc tcaacatatt gaaatccggc agacgaatca tcatcacgat
    40321 cccgtgggta cctactttct cctgatcttc cagtaacgtt gctaactgat tatcgaaaaa
    40381 gagtcggtta ttgaggccgg ttttcacgtc ctgggcggca taagagcgga tcagcgtatc
    40441 aagacggctg tgttgttcgc gtgcgttctg aatttcacga agcagcgtat ccagcgcact
    40501 gctggttctg ggcggccatt catagatggt tcccaacaca ttagagccac gctcaccgtt
    40561 taagatacga gtagcccggg tttccagcaa ttcttgcccg gcaagttgcc gttgtaacca
    40621 gcgtaccgcc aggaagagca taacaatgat aaagccaatc gcccccgtga gcggcgcggt
    40681 ggtcatcaac gaatggaaat agttgcccat cggatcctga taaaccagac gcaacgacat
    40741 ccccggatgc tttatcaacg gaacgctcag ttcgcgaaac agatcgctgg agccaactgg
    40801 acgataacta ccatttctgg ccagggtata aacctgttta tcaccatgga gcaggtctac
    40861 acgaacgata tctgccgaca tcattaattc ggtaatttgt ggccttaata cgctgaagtc
    40921 attcgacaca aggtgggtat caatcgccgt cgccaccgcc tgaacgcgat gactaaactt
    40981 atactgaatg gcgttgtaga aacttagcga acagcccagc aaagtcacaa aaattgttaa
    41041 cccggtgagc agcgtaacaa aggccgaaaa tttcgtcgtt aatctcatcc ttgtgttaac
    41101 tccgatagtg aggaagcggg catactagca aatcagattt atctcgcaat ttattgcgct
    41161 tcatcggctt tgcttttcca ttagcgagta tagtcttcag aaattatttt ccaatccatc
    41221 atgcacatga ggaccactta tgcaggcgtt acttttagaa cagcaggacg gcaaaactct
    41281 cgcatcagta cagactctgg acgaaagtcg cctgccggag ggcgatgtca cggtcgatgt
    41341 tcactggtcg agcctgaact ataaagatgc gctggcgatt accggtaagg gaaaaatcat
    41401 ccgtaatttt ccgatgattc ctgggatcga ttttgccgga actgtacgca ccagcgaaga
    41461 tccgcgtttt catgccggtc aggaggtgtt actcactggc tggggcgttg gtgaaaacca
    41521 ctggggtggg ctggcggagc aggcgcgagt gaaaggtgac tggctggttg ccatgccgca
    41581 agggctggac gcgcgtaaag caatgattat cggtactgcc ggttttaccg ccatgctgtg
    41641 tgtgatggcg ctggaagatg ccggtgttcg cccgcaggac ggggagattg tcgtgacggg
    41701 tgccagtggt ggcgtcggca gtaccgccgt ggcgctgctg cataagttgg gttatcaggt
    41761 cgttgccgtt tccggtcgcg aaagtaccca tgaatatctg aaaagtttag gtgctagccg
    41821 tgttctccct cgtgatgagt ttgccgaatc ccgtcctctg gaaaaacaag tctgggctgg
    41881 ggcaattgac accgttggcg acaaagtgct ggcaaaagtg ctggcgcaaa tgaattacgg
    41941 cggctgcgtg gcggcctgtg gtctggcggg tggttttact ctgccaacca cggtcatgcc
    42001 atttattctg cgtaatgtcc gtttgcaagg ggtggattca gtaatgacgc caccagaacg
    42061 ccgcgcacaa gcctggcagc gactggtcgc cgatttaccg gaatcattct atacccaggc
    42121 ggcaaaagag atatctctgt cagaggcacc gaactttgcc gaggccatca ttaataacca
    42181 gatccagggt cgcacgctgg tgaaggttaa ctaaccattt agcagggaat aataagagag
    42241 ggaactcatt ttgaaatcat tattcaatcg gttaacggga aaagcggtta gccggacagc
    42301 tttcgtcgaa caccttggtc aggaagttat acaacatcat ccaaactgga aagtcatgat
    42361 ttcgactgac cacaaattga tgcgcattga tactccacta aacagctatt attgatacgc
    42421 ctccgtcgcc tgttaggttt atggtgcttt gcctgggcga cgctacgctt agccccttac
    42481 ttatttctgg taccatgggg tgaataatct gattttgttt gactacaaat taatcactcg
    42541 aacctattta atgctgagca ttgtcaatcg gttaattttg cgtgctttag cattcacatc
    42601 tatccagacg atgcagtgaa aattgggtaa tccccagcaa ccgctgcgta atgtcgtcta
    42661 tcttgtcgcg atcctggcat ccctacatta tttgtggtct gtgaagatta tctcattgca
    42721 gcccctcatc ttcgcagggc tggcttttca gcttttcacc ttacgttata agaagttccg
    42781 tcgatgatgg cgctaatttc gtgaattgtg cggcttgttg caaattacac ggtgttgaag
    42841 gttatttaca tgttagctgt tgattatctt ccctgataag accagtattt agctgccaat
    42901 tgctacgaaa tcgttataat gtgcgacctc gtcctccctg acgcagtttt tgcgctgcgg
    42961 aaaaggtgac attggcgcaa cgaaggtata ttttgttttt tgccggagga tagcagcaga
    43021 tcgctgcaca atgtccgtca agtctaacat tgacactctg gggcaaaata gaccggcgtc
    43081 ccggcctgct ggaatttatc gctatgcata cagctgtcgg ggcatacgct ttacagacgg
    43141 cggtgaaacg cctgtcacaa tcacactaaa caaagagtac ggaacccact catggatatt
    43201 cgtaagatta aaaaactgat cgagctggtt gaagaatcag gcatctccga actggaaatt
    43261 tctgaaggcg aagagtcagt acgcattagc cgtgcagctc ctgccgcaag tttccctgtg
    43321 atgcaacaag cttacgctgc accaatgatg cagcagccag ctcaatctaa cgcagccgct
    43381 ccggtgaccg ttccttccat ggaagcgcca gcagcagcgg aaatcagtgg tcacatcgta
    43441 cgttccccga tggttggtac tttctaccgc accccaagcc cggacgcaaa agcgttcatc
    43501 gaagtgggtc agaaagtcaa cgtgggcgat accctgtgca tcgttgaagc catgaaaatg
    43561 atgaaccaga tcgaagcgga caaatccggt accgtgaaag caattctggt cgaaagtgga
    43621 caaccggtag aatttgacga gccgctggtc gtcatcgagt aacgaggcga acatgctgga
    43681 taaaattgtt attgccaacc gcggcgagat tgcattgcgt attcttcgtg cctgtaaaga
    43741 actgggcatc aagactgtcg ctgtgcactc cagcgcggat cgcgatctaa aacacgtatt
    43801 actggcagat gaaacggtct gtattggccc tgctccgtca gtaaaaagtt atctgaacat
    43861 cccggcaatc atcagcgccg ctgaaatcac cggcgcagta gcaatccatc cgggttacgg
    43921 cttcctctcc gagaacgcca actttgccga gcaggttgaa cgctccggct ttatcttcat
    43981 tggcccgaaa gcagaaacca ttcgcctgat gggcgacaaa gtatccgcaa tcgcggcgat
    44041 gaaaaaagcg ggcgtccctt gcgtaccggg ttctgacggc ccgctgggcg acgatatgga
    44101 taaaaaccgt gccattgcta aacgcatagg ttatccggtg attatcaaag cctccggcgg
    44161 cggcggcggt cgtggtatgc gtgtagtgcg cggcgacgct gaactggcac aatccatctc
    44221 catgacccgt gcggaagcga aagctgcttt cagcaacgat atggtttaca tggagaaata
    44281 cctggaaaat cctcgccacg tcgagattca ggtactggct gacggtcagg gcaacgctat
    44341 ctatctggcg gaacgtgact gctccatgca gcgccgccac cagaaagtgg tcgaagaagc
    44401 accagcaccg ggcattaccc cggaactgcg tcgctacatc ggcgaacgtt gcgctaaagc
    44461 gtgtgttgat atcggctatc gcggtgcagg tactttcgag ttcctgttcg aaaacggcga
    44521 gttctatttc atcgaaatga acacccgtat tcaggtagaa cacccggtta ccgaaatgat
    44581 cactggcgtt gacctgatca aagaacagct gcgtatcgct gccggtcaac cgctgtcgat
    44641 caagcaagaa gaagttcacg ttcgcggcca tgcggtggaa tgtcgtatca acgccgaaga
    44701 tccgaacacc ttcctgccaa gcccgggcaa aatcacccgt ttccacgcac ctggcggttt
    44761 tggcgtacgt tgggagtctc atatctacgc gggctacacc gtaccgccgt actatgactc
    44821 aatgatcggt aagctgattt gctacggtga aaaccgtgac gtggcgattg ctcgcatgaa
    44881 gaatgcgctg caggagctga tcatcgacgg tatcaaaacc aacgttgatc tgcagatccg
    44941 cataatgaat gacgagaact tccagcatgg tggtactaac atccactatc tggagaaaaa
    45001 actcggtctt caggaaaaat aagactgcaa aagcgtcaaa aggccggatt ttccggcctt
    45061 ttttattact ggggatcgac aacccccata aggtacaatc ctcgctttct tcacccatca
    45121 gggacaaaaa atggacactc gttttgttca ggcccataaa gaggcgcgct gggcgctggg
    45181 gctgaccctt ttgtatctgg cagtttggtt agcagccgct tacttacctg gcgttgcccc
    45241 cggttttacc ggctttccgc gctggtttga gatggcctgc atcctgacgc cgctgctgtt
    45301 tattggactg tgctgggcga tggtgaaatt tatctatcgc gatatcccac tggaggatga
    45361 cgatgcagct tgaagtaatt ctaccgctgg tcgcctatct ggtggtggtg ttcggtatct
    45421 cggtttatgc gatgcgtaaa cggagcaccg gcaccttcct taatgagtat ttcctcggca
    45481 gccgctctat gggcggtatt gtgctggcga tgacgctcac cgcgacctat atcagtgcca
    45541 gttcgtttat cggcgggcca ggagctgctt ataaatacgg gctgggctgg gtattgctgg
    45601 cgatgattca gcttcctgca gtctggcttt cactcggtat tctcggcaag aagtttgcga
    45661 ttcttgcgcg ccgctacaat gcagtgacgc tgaacgatat gctgtttgcc cgctaccaga
    45721 gtcgtcttct ggtgtggctg gcgagtttga gtttgctggt tgcgttcgtt ggtgcgatga
    45781 ccgtgcagtt tatcggcggt gcgcgcctgc tggaaaccgc ggcgggtatt ccttatgaaa
    45841 ccgggctgct gatttttggt atcagcattg cgttatatac cgcctttggt ggctttcgcg
    45901 ccagcgtgct gaacgacacc atgcaagggc ttgtgatgct gattggcacc gttgtgctgc
    45961 ttattggcgt ggtacatgcc gctggcggct taagcaacgc agtacagacc ttgcaaacca
    46021 tcgatccgca actggttacg ccacaaggcg ctgacgatat tctgtcgcct gcctttatga
    46081 cgtcgttctg ggtactggtg tgctttggcg tgattggcct gccgcatact gcggtgcgct
    46141 gtatctctta taaagacagc aaagcagtac accgggggat catcatcggt acgattgtag
    46201 tcgcaatcct gatgttcggt atgcatctag ccggagcgtt aggtcgtgca gtgatccccg
    46261 atctcaccgt accggatctg gtgatcccga cgttaatggt aaaagtgctg ccaccttttg
    46321 ctgccgggat ctttctggct gcacctatgg ctgcgatcat gtcgacaatt aacgcccaac
    46381 tgctgcaaag ttccgctacg atcattaaag atctctatct gaatatccgt ccggatcaaa
    46441 tgcaaaacga gacgcgtctg aagcggatgt cggcggtaat tacgttagtt ctcggcgcgt
    46501 tgctgctgct tgccgcctgg aagccgccag aaatgatcat ctggctgaat ttgctggcct
    46561 tcggtgggct ggaagccgtt ttcctgtggc cgctggtgct gggtctttac tgggaacgcg
    46621 ccaacgccaa aggcgcgcta agtgcgatga tcgttggcgg cgtgctgtat gccgtactcg
    46681 cgacgctgaa tattcagtac ctgggcttcc accctatcgt gccctcgtta ctactaagtt
    46741 tgctggcttt cctggtcgga aaccgtttcg gtacatccgt cccgcaagct accgttttga
    46801 ctactgataa ataaagagtt ttgccatgcc ttggatccaa ctgaaactga acaccaccgg
    46861 cgcgaacgcg gaagatctta gcgatgcgct gatggaagcg ggtgccgttt ctatcacttt
    46921 tcaggatacc cacgatacgc cagtatttga gccgctgccg ggcgaaacgc gcctgtgggg
    46981 cgacaccgat gtgattggtc tgttcgacgc tgaaaccgat atgaacgacg tggtggcgat
    47041 tctggaaaac catccgctgc tcggcgcagg cttcgcgcat aaaatcgaac aactagaaga
    47101 taaagactgg gagcgcgaat ggatggataa tttccacccg atgcgctttg gtgaacgact
    47161 gtggatctgc cctagctggc gtgatgtgcc ggacgaaaac gccgtcaacg tgatgttaga
    47221 tccagggctg gcgtttggta cgggtaccca tccaaccacc tctctgtgcc tgcaatggct
    47281 cgacagcctc gatttaaccg gtaaaacagt catcgacttt ggctgtggtt ccggcattct
    47341 ggcgatcgcg gcgctgaaac tgggtgcagc aaaagccatt ggtattgata tcgatccgca
    47401 ggcgattcag gccagccgcg ataacgccga acgtaatggc gtttctgacc gtctggaact
    47461 ctacttaccg aaagatcagc cagaagaaat gaaagccgac gtggtggtcg ctaacatcct
    47521 tgcaggccca ttacgtgaac tggcaccgtt aatcagcgtc ctgccggttt caggcggttt
    47581 gctgggcctt tccggtattc tggcaagcca ggcagagagc gtttgtgaag cttatgccga
    47641 cagcttcgca ctggacccgg tcgtggaaaa agaagagtgg tgccgcatta ccggtcgtaa
    47701 gaattaacct tcgcatcgcc gtagggtgac gcggggcgaa gtgcgagcaa gctcacaaaa
    47761 ggcacgtaaa tttgccgatt atttacgcaa atttgcgtgc caaaattttc attcataaag
    47821 aaaaattgag aacttactca aatttctttg agtgtaaatt ttagtcacta ttttctaata
    47881 tgatgatttt tatgagtaat tatcgcacca cgctcatttt aaatgcaatt ctttgatcca
    47941 tctcagagga ttggtcaaag tttggccttt catctcgtgc aaaaaatgcg taatatacgc
    48001 cgccttgcag tcacagtatg gtcatttctt aactcatgcg catcggacaa tatcagctca
    48061 gaaatcgcct gatcgcagcg cccatggctg gcattacaga cagacctttt cggacgttgt
    48121 gctacgagat gggagccgga ttgacagtat ccgagatgat gtcttctaac ccacaggttt
    48181 gggaaagcga caaatctcgt ttacggatgg tgcacattga tgaacccggt attcgcaccg
    48241 tgcaaattgc tggtagcgat ccgaaagaaa tggcagatgc agcacgtatt aacgtggaaa
    48301 gcggtgccca gattattgat atcaatatgg gttgcccggc taaaaaagtg aatcgcaagc
    48361 tcgcaggttc agcccttttg cagtacccgg atgtcgttaa atcgatcctt accgaggtcg
    48421 tcaatgcagt ggacgttcct gttaccctga agattcgcac cggctgggca ccggaacacc
    48481 gtaactgcga agagattgcc caactggctg aagactgtgg cattcaggct ctgaccattc
    48541 atggccgtac acgcgcctgt ttgttcaatg gagaagctga gtacgacagt attcgggcag
    48601 ttaagcagaa agtttccatt ccggttatcg cgaatggcga cattactgac ccgcttaaag
    48661 ccagagctgt gctcgactat acaggggcgg atgccctgat gataggccgc gcagctcagg
    48721 gaagaccctg gatctttcgg gaaatccagc attatctgga cactggggag ttgctgcccc
    48781 cgctgccttt ggcagaggtt aagcgcttgc tttgcgcgca cgttcgggaa ctgcatgact
    48841 tttatggtcc ggcaaaaggg taccgaattg cacgtaaaca cgtttcctgg tatctccagg
    48901 aacacgctcc aaatgaccag tttcggcgca cattcaacgc cattgaggat gccagcgaac
    48961 agctggaggc gttggaggca tacttcgaaa attttgcgta aacagaaata aagagctgac
    49021 agaactatgt tcgaacaacg cgtaaattct gacgtactga ccgtttctac cgttaactct
    49081 caggatcagg taacccaaaa acccctgcgt gactcggtta aacaggcact gaagaactat
    49141 tttgctcaac tgaatggtca ggatgtgaat gacctctatg agctggtact ggctgaagta
    49201 gaacagcccc tgttggacat ggtgatgcaa tacacccgtg gtaaccagac ccgtgctgcg
    49261 ctgatgatgg gcatcaaccg tggtacgctg cgtaaaaaat tgaaaaaata cggcatgaac
    49321 taattcaggt tagctaaatg cttgattaaa aaggcgctac tcggcatggg gcagcgcctt
    49381 ttttataggt gtcacaaagg gagtgaccat gagaacagga tgtgaaccga cccggtttgg
    49441 taatgaagct aagaccatta ttcacggtga tgcccttgcc gaacttaaaa agctacctac
    49501 tgaaagcgtc gatctgatct ttgccgaccc accgtataac atcggtaaaa attttgatgg
    49561 tctgatcgaa gcctggaaag aagatctgtt tatcgactgg ctgtttgaag tgattgcaga
    49621 gtgccaccgc gttctgaaaa agcagggcag catgtacatt atgaacagta cggaaaacat
    49681 gccctttatc gatctccagt gccgcaagct ttttaccatc aaaagtcgca tcgtctggtc
    49741 atatgacagt tctggagtac aggcgaaaaa acactacggc tccatgtacg a
//
LOCUS       DAZFZC010000024        49680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFZC010000024 DAZFZC010000000
VERSION     DAZFZC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.024"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(87..1217)
                     /gene="dnaJ"
                     /locus_tag="ACHKXP_001630"
     CDS             complement(87..1217)
                     /gene="dnaJ"
                     /locus_tag="ACHKXP_001630"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO7646664.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1306..3222)
                     /gene="dnaK"
                     /locus_tag="ACHKXP_001631"
     CDS             complement(1306..3222)
                     /gene="dnaK"
                     /locus_tag="ACHKXP_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO7646665.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3599..4003
                     /gene="yaaI"
                     /locus_tag="ACHKXP_001632"
     CDS             3599..4003
                     /gene="yaaI"
                     /locus_tag="ACHKXP_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO7646666.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4029..4742
                     /gene="msyB"
                     /locus_tag="ACHKXP_001633"
     CDS             4029..4742
                     /gene="msyB"
                     /locus_tag="ACHKXP_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7646667.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     HNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4891..5457
                     /gene="satP"
                     /locus_tag="ACHKXP_001634"
     CDS             4891..5457
                     /gene="satP"
                     /locus_tag="ACHKXP_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO7646668.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5492..6079)
                     /gene="mog"
                     /locus_tag="ACHKXP_001635"
     CDS             complement(5492..6079)
                     /gene="mog"
                     /locus_tag="ACHKXP_001635"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO7646669.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6194..7147)
                     /gene="tal"
                     /locus_tag="ACHKXP_001636"
     CDS             complement(6194..7147)
                     /gene="tal"
                     /locus_tag="ACHKXP_001636"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7646670.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7426..8858
                     /gene="yaaJ"
                     /locus_tag="ACHKXP_001637"
                     /pseudo
     CDS             7426..8858
                     /gene="yaaJ"
                     /locus_tag="ACHKXP_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            8928..9704
                     /gene="yaaA"
                     /locus_tag="ACHKXP_001638"
     CDS             8928..9704
                     /gene="yaaA"
                     /locus_tag="ACHKXP_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO7646671.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDNFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(9857..10153)
                     /gene="yaaX"
                     /locus_tag="ACHKXP_001639"
     CDS             complement(9857..10153)
                     /gene="yaaX"
                     /locus_tag="ACHKXP_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO7646672.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10367..11653)
                     /gene="thrC"
                     /locus_tag="ACHKXP_001640"
     CDS             complement(10367..11653)
                     /gene="thrC"
                     /locus_tag="ACHKXP_001640"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO7646673.1"
                     /translation="MKLYNLKDHSEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11654..12586)
                     /gene="thrB"
                     /locus_tag="ACHKXP_001641"
     CDS             complement(11654..12586)
                     /gene="thrB"
                     /locus_tag="ACHKXP_001641"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO7646674.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12588..15050)
                     /gene="thrA"
                     /locus_tag="ACHKXP_001642"
     CDS             complement(12588..15050)
                     /gene="thrA"
                     /locus_tag="ACHKXP_001642"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO7646675.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSFIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRSLELADIEIESVLPAEFNAEGDVATFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDVDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15077..15196)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15132..15197)
                     /gene="thrL"
                     /locus_tag="ACHKXP_001643"
     CDS             complement(15132..15197)
                     /gene="thrL"
                     /locus_tag="ACHKXP_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO7646676.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15411..16097)
                     /gene="yjtD"
                     /locus_tag="ACHKXP_001644"
     CDS             complement(15411..16097)
                     /gene="yjtD"
                     /locus_tag="ACHKXP_001644"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7646677.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNVKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDTKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16497..16637)
                     /gene="yjjY"
                     /locus_tag="ACHKXP_001645"
     CDS             complement(16497..16637)
                     /gene="yjjY"
                     /locus_tag="ACHKXP_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO7646678.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16733..17449
                     /gene="arcA"
                     /locus_tag="ACHKXP_001646"
     CDS             16733..17449
                     /gene="arcA"
                     /locus_tag="ACHKXP_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO7646679.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17508..18860)
                     /gene="creD"
                     /locus_tag="ACHKXP_001647"
     CDS             complement(17508..18860)
                     /gene="creD"
                     /locus_tag="ACHKXP_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO7646680.1"
                     /translation="MLKSPLFWKMTPLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18918..20342)
                     /gene="creC"
                     /locus_tag="ACHKXP_001648"
     CDS             complement(18918..20342)
                     /gene="creC"
                     /locus_tag="ACHKXP_001648"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO7646681.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20342..21031)
                     /gene="creB"
                     /locus_tag="ACHKXP_001649"
     CDS             complement(20342..21031)
                     /gene="creB"
                     /locus_tag="ACHKXP_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO7646682.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21044..21517)
                     /gene="creA"
                     /locus_tag="ACHKXP_001650"
     CDS             complement(21044..21517)
                     /gene="creA"
                     /locus_tag="ACHKXP_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO7646683.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21728..22597
                     /gene="robA"
                     /locus_tag="ACHKXP_001651"
     CDS             21728..22597
                     /gene="robA"
                     /locus_tag="ACHKXP_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO7646684.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22594..23241)
                     /gene="gpmB"
                     /locus_tag="ACHKXP_001652"
     CDS             complement(22594..23241)
                     /gene="gpmB"
                     /locus_tag="ACHKXP_001652"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO7646685.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23293..23814
                     /gene="yjjX"
                     /locus_tag="ACHKXP_001653"
     CDS             23293..23814
                     /gene="yjjX"
                     /locus_tag="ACHKXP_001653"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO7646686.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(23899..24225)
                     /gene="trpR"
                     /locus_tag="ACHKXP_001654"
     CDS             complement(23899..24225)
                     /gene="trpR"
                     /locus_tag="ACHKXP_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO7646687.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24315..26252)
                     /gene="sltY"
                     /locus_tag="ACHKXP_001655"
     CDS             complement(24315..26252)
                     /gene="sltY"
                     /locus_tag="ACHKXP_001655"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO7646688.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26463..28130
                     /gene="ettA"
                     /locus_tag="ACHKXP_001656"
     CDS             26463..28130
                     /gene="ettA"
                     /locus_tag="ACHKXP_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO7646689.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28291..28398)
                     /gene="nadS"
                     /locus_tag="ACHKXP_001657"
                     /pseudo
     CDS             complement(28291..28398)
                     /gene="nadS"
                     /locus_tag="ACHKXP_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(28437..29669)
                     /gene="nadR"
                     /locus_tag="ACHKXP_001658"
     CDS             complement(28437..29669)
                     /gene="nadR"
                     /locus_tag="ACHKXP_001658"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO7646690.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(29690..31072)
                     /gene="radA"
                     /locus_tag="ACHKXP_001659"
     CDS             complement(29690..31072)
                     /gene="radA"
                     /locus_tag="ACHKXP_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO7646691.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARDERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKMRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31121..32089)
                     /gene="serB"
                     /locus_tag="ACHKXP_001660"
     CDS             complement(31121..32089)
                     /gene="serB"
                     /locus_tag="ACHKXP_001660"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO7646692.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32195..32839
                     /gene="ytjB"
                     /locus_tag="ACHKXP_001661"
     CDS             32195..32839
                     /gene="ytjB"
                     /locus_tag="ACHKXP_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO7646693.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32867..33883
                     /gene="lplA"
                     /locus_tag="ACHKXP_001662"
     CDS             32867..33883
                     /gene="lplA"
                     /locus_tag="ACHKXP_001662"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO7646694.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            33915..34178
                     /locus_tag="ACHKXP_001663"
                     /pseudo
     CDS             33915..34178
                     /locus_tag="ACHKXP_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34339..35058)
                     /gene="deoD"
                     /locus_tag="ACHKXP_001664"
     CDS             complement(34339..35058)
                     /gene="deoD"
                     /locus_tag="ACHKXP_001664"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO7646695.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35138..36361)
                     /gene="deoB"
                     /locus_tag="ACHKXP_001665"
     CDS             complement(35138..36361)
                     /gene="deoB"
                     /locus_tag="ACHKXP_001665"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7646696.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36413..37735)
                     /gene="deoA"
                     /locus_tag="ACHKXP_001666"
     CDS             complement(36413..37735)
                     /gene="deoA"
                     /locus_tag="ACHKXP_001666"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO7646697.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(37862..38641)
                     /gene="deoC"
                     /locus_tag="ACHKXP_001667"
     CDS             complement(37862..38641)
                     /gene="deoC"
                     /locus_tag="ACHKXP_001667"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO7646698.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            38899..40449
                     /gene="yjjI"
                     /locus_tag="ACHKXP_001668"
     CDS             38899..40449
                     /gene="yjjI"
                     /locus_tag="ACHKXP_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO7646699.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWSYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            40421..41284
                     /gene="yjjW"
                     /locus_tag="ACHKXP_001669"
     CDS             40421..41284
                     /gene="yjjW"
                     /locus_tag="ACHKXP_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO7646700.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKCSIYLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKMRG
                     VSRLIFPALYL"
     gene            complement(41801..42583)
                     /gene="yjjV"
                     /locus_tag="ACHKXP_001670"
     CDS             complement(41801..42583)
                     /gene="yjjV"
                     /locus_tag="ACHKXP_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7646701.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAEQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYGALGLHPGMLEKHSDASLEQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(42580..43653)
                     /gene="yjjU"
                     /locus_tag="ACHKXP_001671"
     CDS             complement(42580..43653)
                     /gene="yjjU"
                     /locus_tag="ACHKXP_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO7646702.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPASVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(43775..43936)
                     /gene="ytjA"
                     /locus_tag="ACHKXP_001672"
     CDS             complement(43775..43936)
                     /gene="ytjA"
                     /locus_tag="ACHKXP_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO7646703.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44063..44668)
                     /gene="osmY"
                     /locus_tag="ACHKXP_001673"
     CDS             complement(44063..44668)
                     /gene="osmY"
                     /locus_tag="ACHKXP_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO7646704.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45061..46650)
                     /gene="prfC"
                     /locus_tag="ACHKXP_001674"
     CDS             complement(45061..46650)
                     /gene="prfC"
                     /locus_tag="ACHKXP_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO7646705.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46741..47418)
                     /gene="yjjG"
                     /locus_tag="ACHKXP_001675"
     CDS             complement(46741..47418)
                     /gene="yjjG"
                     /locus_tag="ACHKXP_001675"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO7646706.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47433..47879)
                     /gene="rimI"
                     /locus_tag="ACHKXP_001676"
     CDS             complement(47433..47879)
                     /gene="rimI"
                     /locus_tag="ACHKXP_001676"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7646707.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47848..48261)
                     /gene="holD"
                     /locus_tag="ACHKXP_001677"
     CDS             complement(47848..48261)
                     /gene="holD"
                     /locus_tag="ACHKXP_001677"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO7646708.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKISMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48364..49395
                     /gene="rsmC"
                     /locus_tag="ACHKXP_001678"
     CDS             48364..49395
                     /gene="rsmC"
                     /locus_tag="ACHKXP_001678"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO7646709.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPEFDADKFWG
                     EYNVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            49595..49678
                     /locus_tag="ACHKXP_001679"
     tRNA            49595..49678
                     /locus_tag="ACHKXP_001679"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49629..49631,aa:Leu,seq:cag)
BASE COUNT        11592 a        13530 c        12583 g        11975 t
ORIGIN      
        1 gatccccgcc acttttcagg tgctcgcata tcttcaacgc accctaattt tacccaagcc
       61 tgcccacggg caggcttttg gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca
      121 ccatcaaaga agctctttga gcgcgggctg ttgtgctcac cggttgggcc accgaagctt
      181 tcttgcagct cttgcagcag ctgtttctgc ttctcgttca ggcctaccgg tgtttcgacg
      241 acaacgcggc acagtaaatc accctgtgcg ccaccgcgga cagacttgac gcctttaccg
      301 cgcatacgga acagcttacc agtctgggtt tcgccaggca ctttcagttt gacgcgacca
      361 tcaagggtcg gtacttcgat ttcgccaccc agcgccgcca tagcgaagtt gatcgggact
      421 tcgcaataca ggttgttgcc ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga
      481 acgtacagat cgcctgccgg tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg
      541 atgcggtctc cagtgtccac ccctgccggg attttaacgg acagcgtttt gctgcgctca
      601 acacgaccat gaccatgaca tttgttgcac ggatctttga tcagcgtacc gcggccctga
      661 cagtgtggac aggtctgctg cacggcaaag aaaccctggc gcatctgcac ctgaccagaa
      721 ccatgacagg tcggacaggt ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa
      781 acgtcacact cttccagagt cggaatgcgg atctctttgg tcacgccacg tacagcttct
      841 tcgagggtga gctccatgtt atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca
      901 cgtccgccgc caaaaatatc gccaaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg
      961 ccgccaaaac cgccgccgcc catgccgcct tgctcaaacg cagcatgacc atactgatcg
     1021 tatgccgcac gtttttgcga gtcggtcaga acttcataag cttccttgat ctctttaaat
     1081 ttcgcctcgg cctctttgtc accctggtta cggtccgggt ggtatttcat ggccaggcgt
     1141 ttgtaggcct ttttgatttc acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa
     1201 taatcttgct tagccatctt ttttaaattg cccctagatg aatgcacggg cggagaggaa
     1261 attccccttc gcccgtgtca gtataattac ccgtttatag ggcgattatt ttttgtcttt
     1321 gacttcttca aattcagcgt cgacaacatc gtcatctttc gcgttgtttg cagaagcatc
     1381 agcaccggca gtctgctgct gggcatgttg ctgctgggcg atttccatca gtttctggga
     1441 aacctgtgcc agctcctgca ttttcgcttc gatagcggct ttgtcttcac ctttcagagc
     1501 agtttccagt gcagtcagtg cagactcgat agcagttttg tcgtcagccg gcagtttgtc
     1561 gcctgcttct tcaacctgct tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt
     1621 ctgtaccagc tcttcaaact tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat
     1681 tttctggatt tcatcttcgt tcagaccaga agaagccttg atggtgatct tctgctcttt
     1741 accgctgttt ttatctttcg cggaaacgtg caggataccg tcagcatcga tatcgaaggt
     1801 aacttcgatc tgcggcatgc cgcgcggtgc cgggttgata ccatccaggt tgaactgacc
     1861 cagagatttg ttatcagccg cacgtttacg ttcaccctgc agcacatgga tggttaccgc
     1921 agactggttg tcttcagcgg tagagaacac ctggctgtgc ttggtcggga tagtggtgtt
     1981 tttcgcgatc agcgtcgtca tcacaccgcc catggtttcg atacccagag acagcggggt
     2041 aacgtccagc agcagtacgt cttttacgtc accagtcaga acaccaccct gaacagcagc
     2101 accgattgct acagcttcgt ccgggttaac gtctttacgc ggctctttac caaagaactc
     2161 agcaactttc ttctgaacca ttggcatacg agtctgacca ccaacgagga taacgtcgtc
     2221 gatatcagat acggacaggc cagcgtcctg cagtgcaact ttcagcggct caatggaacg
     2281 gtttaccaga tcttcaacca ggctttccag tttcgcacga gtcactttga tgttcatgtg
     2341 tttcggaccg gtcgcgtctg cagtgatgta cggcaggtta acgtcggtct gctgagcgga
     2401 agacagttcg attttcgctt tttctgccgc ttctttcagg cgctgcattg ccagcggatc
     2461 gttgcgcagg tcaatgccct gatctttctt gaattcttca accagatagt tgatcagacg
     2521 gctgtcgaag tcttcaccac ccaggtgggt atcaccgttg gttgccaaaa cttcgaaggt
     2581 tttttcgccg tcaacttcgt cgatttcgat aatagaaata tcgaaagtac caccacccag
     2641 gtcataaacc gcgatagtac ggttgccagt acctttgtcc agaccgtaag ccagcgcagc
     2701 tgcggtcggt tcgttgatga tacgttttac ttccagacca gcgatacggc ctgcgtcttt
     2761 ggttgcctga cgctgagcat cgttaaagta tgccggtacg gtgataacag cttcagttac
     2821 cggttcaccc aagtaatctt cagcggtttt cttcattttt ttcagcactt cagcagaaat
     2881 ctgcggcggt gccattttct ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc
     2941 aataattttg aacggcatga tggaaacatc acgctgtact tcttcgtcct ggaagcggcg
     3001 gccaatcagg cgtttaatcg caaacagggt gttttgcggg ttcgtcactg cctgacgttt
     3061 agccggctga ccaaccagag tttcaccatc ctgggtatag gcaataatag aaggcgtggt
     3121 gcgatcgcct tcggcgttct ccagcacgcg aggagtggtg ccatccataa tcgctacaca
     3181 agagttggta gtacccaggt cgataccaat tattttaccc atctaaacgt ctccactata
     3241 tattcggtca tcatgtggtt gtgagtctgt aataggggcg aaacgtctgg tttcaactgc
     3301 ccaatttcat ttttttgcag actcactcac tgcggttgac tactaaatgg ggtcgtaacc
     3361 cacgtcatca agggggagat gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata
     3421 tggcaggaca agacgtgaaa ctgacagcaa ttttattcat ataaagaatg aatcgcagcc
     3481 aaggcattca tcaaaaaatt gtaataaaaa gaaaacatta cgcacctgaa tcttctcttt
     3541 atcagcagta aactagtggg tatccatccc cctacctctc cccactaaga gaatccttat
     3601 gaaatccgtt tttacgattt ccgccagcct ggcgattagt ctgatgctgt gctgcacggc
     3661 gcaggcaaac gaccataaaa tcctcggcgt cattgcgatg ccacgtaacg aaaccaacga
     3721 tctggcgctg aaacttcctg tttgccgaat tgttaaacgc atacaactct ctgccgacca
     3781 tggcgattta cagttaagcg gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca
     3841 gagtctgaat attccttcag aaataaaaga agggcaaacc actgactgga tcaacattaa
     3901 cagcgataac gacaataaac gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa
     3961 ctcatcggat atggccacgc tgaaaattat cggcgacgac taacgccaga atatagaagc
     4021 tacaaaaaat gaatgttaat tacctgaatg attcagatct ggattttctt cagcattgta
     4081 gtgaggaaca gttggcaaat ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc
     4141 gcctctccag cgtactgatg cacaacgaac tgtttaaatc gatggaaggg catcccgagc
     4201 aacatcgccg caactggcag ctgattgctg gagaattaca gcattttggt ggcgatagta
     4261 tcgccaacaa actgcgcgga cacggtaaat tgtatcgggc cattttgctc gatgtttcaa
     4321 aacgattgaa gctgaaagcc gacaaagaga tgtctacgtt tgagattgag caacagttac
     4381 tggaacaatt tctgcgtaac acctggaaga atatggacga ggaacataag caggagtttc
     4441 tgcacgcggt cgatgccagg gtgaatgagc tggaagagct gctgccgctg ctgatgaaag
     4501 acaaattatt ggcaaaaggc gtgtcgcatt tgctttccag ccaattgacc cgcattttac
     4561 gcacccacgc agcaatgagc gtgcttgggc atggtttgct gcgcggcgcg ggtctgggag
     4621 gccctgtggg cgcagcatta aatggggtta aagcggtcag cggcagtgcc tatcgcgtga
     4681 caattccagc cgtactgcaa atcgcctgcc tgcgccggat ggttagcgcc actcaggtct
     4741 gacaccgtaa accggggaaa attttcattt tcccctcaaa agatcgtaga cactgcccca
     4801 ctggctgatt attatgccgc gccctgaaaa cactactgtt attcagggaa attatttcac
     4861 cattcattcg atgatgattt ttgaggaatt atgggcaaca ctaagttggc taatccggca
     4921 ccgctgggcc tgatgggctt cggcatgacc accattctgc ttaacctgca caacgtgggt
     4981 tatttcgctc tggacggtat tattcttgcc atgggcattt tctacggcgg catcgcgcaa
     5041 atttttgccg gtctgctgga gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc
     5101 tcttacggtt ctttctggct gacgctggtt gcgattctgc tgatgccgaa actgggtctg
     5161 accgatgcgc caaatgcaca gttccttggt gtctacctgg gtctgtgggg cgtatttacg
     5221 ctgtttatgt tcttcggcac gctgaaaggc gcacgcgttc tgcaattcgt tttctttagc
     5281 ctgactgtgc tgtttgccct gctggcgatc ggtaacattg ccggtaacgc cgcaatcatc
     5341 cactttgccg gctggattgg tctgatctgt ggtgccagtg caatctatct ggcgatgggt
     5401 gaagtactga acgagcagtt tggtcgcacc gttctgccga ttggtgaatc ccactaattg
     5461 ttttgagatc cccccgctcg gggggatttt tttattcgct aacgtcgcgt cttgcactct
     5521 tcggtctgaa tgcagcaacc acttctggtg ccgtttcaac gtatggccct tccagcaact
     5581 gaatgcagta cggtacgctg gcaaaaatac cgtgtaccac aacgttaccc gcagcgtcct
     5641 tcacaccttc cagcgtctct ttaatagact tcggctgacc gggtaagtta aggatcagcg
     5701 cctgtttgcg aatcacacca acctgacgcg aaaggatcgc agttggtaca aaatgcaggc
     5761 ttatctggcg catctgttca ccaaagccag gcatctcgcg gtccgctact gccagcgtcg
     5821 catcgggcgt tacgtcacga cgcgccgggc cagttccgcc cgtggtgagc accagatggc
     5881 aactcatttc atccactagc tcacacaacg tttgctcgat gatcgcctgc tcatcgggga
     5941 ttaagcgggt ttccagttca aacggcgtgg ttagcgccga tgtcagccat tcttccagcg
     6001 cagggatgcc tttatcctga taaacgccgc tggatgcgcg atcagagatg gaaactaagc
     6061 caatacgtaa agtattcatc aagatttttc cgtttaaaca gtctcgttaa acagaatgat
     6121 acactgcgaa gggagtgaca gacaggcttc agaagaggta gcgtgaccga cttcccggtc
     6181 acgctaagaa tgattacagc agatcgccga tcattttttc cagtttttcc tggtcaatag
     6241 caaacttacg gataccttcc gccagtttat ctactgccat tggatcctgg ttgtgctgcc
     6301 acaggaactc ggactcagtg atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca
     6361 gtttacgttc gatagcccct tcgctctcag ccagctcttt cagcagtgta ggtgcgatgg
     6421 taagacggtc gcagcctgcc agttccagaa tttcgccgat gttacggaag cttgcgccca
     6481 taaccacggt ttcataaccg tgctctttgt agtactggta gatttcagat acagaaacca
     6541 cgcccggatc ttctgccgga gcgtactctt tcttatcggt attcgctttg taccagtcaa
     6601 gaatacggcc aacaaacggc gagatcagga acacgcccgc ttccgcacaa gcacgagcct
     6661 gagcgaagga gaacagcagg gtcaggttac agttgatgcc ttctttttcc agctgttctg
     6721 cagcacggat accctgccag gtagaagcca gtttgatcag aatacgatcg ttgctaatac
     6781 cagcatcgtt gtagagtttg atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat
     6841 aggaaagacg cgcatcaact tcagttgaga tacggcccgg aaccagtttc aggatttcca
     6901 gaccaatatt tactgccagt ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc
     6961 tctgctgttt cgcccaggcg acagcatcat caatcaactt acggtattcc ggaatctgcg
     7021 ctgcgttaag aatgagagaa gggttggttg tggcatcctg cggttgatac agcttcattg
     7081 ccgcgatgtc cccagtgtcg gccactacgg tggtgtactg acgaagggag gtcaatttgt
     7141 ccgtcatgat agtatttctc tttaaacagc ttgttagggg gatgtaaccg gtctgccctg
     7201 atgatatcac gacgcaacgt cagcgcaacc gccgacaaga cggttatcgc caggcgagac
     7261 tgtttcggat ttctgacact gcgttgatat cgcccgccat ttttatacaa aacctcatgt
     7321 atgctacgca gaagttatca agtacctcgt agcgtatata cttcttaaac aattggtaac
     7381 gtttacacag gaaagtcatc gcgaccggca ataagaggga tatgcatgcc agattttttc
     7441 tcctttatta acagcgtcct ttggggatcg gtaatgattt acctgctctt cggcgcaggt
     7501 tgttggttca cttttcgcac cggatttgtg cagtttcgct acatccgcca gtttggcaaa
     7561 agtcttaaaa atagcacatt catccacagc caggcggttt aacctcattt cagtcattgt
     7621 gtaccagtct tgcggcgcgc gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta
     7681 ccgccggtgg acctggagcc gtcttctgga tgtgggttgc cgcgtttatc ggcatggcga
     7741 cctcgtttgc cgaatgttcc ctcgcacagc tttataaaga acgtgatgcc aatgggcagt
     7801 ttcgtggcgg accggcatgg tatatggcgc gcgggctggg gatgcgctgg atgggcgttc
     7861 tgttcgccgt ctttttgctc atcgcctacg gcataatttt cagcggaatt caggcgaacg
     7921 ccgttgcgcg agccctgagt ttttcttttg attttccccc gctggtgaca ggtattattc
     7981 tcgctgtctt tgctctgctg gcgatcactc gcggtcttca tggcgtcgcc cggctcatgc
     8041 aggggtttgt cccgttgatg gcgataatct gggtactgac cagcctggtg atttgcgtaa
     8101 tgaatatcgg gcaacttccc cacgtcattt ggtctatttt tgagagtgct tttggctggc
     8161 aggaagcggc aggcggcgcg gcgggatata ccttaagcca ggcgattact aacggttttc
     8221 agcgcagtat gttttccaat gaggcgggaa tggggtcgac gccaaacgcg gcagcggcag
     8281 cggcgtcctg gcctccgcat ccggcagcgc aagggattgt ccagatgatt ggcattttta
     8341 tcgacaccct ggtcatctgt acggcaagcg ccatgctgat attactggcg ggtaacggca
     8401 caacctacat gccgctggaa ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg
     8461 gttcctgggg tgctgagctt gttaccctcg tggttattct gtttgccttc agctccatcg
     8521 ttgccaacta catttacgcc gaaaacaatc tcttcttttt acgcctgaac aaccctaaag
     8581 cgatctggtg tttgcggatc tgcaccttcg caacggtcat cggcggcacc ttgctaagtc
     8641 ttccgctgat gtggcaactg gcagatatca taatggcctg catggctatt accaatttga
     8701 ccgccatttt actgctctcg cctgtggttc ataccattgc cagtgattat ctacgccagc
     8761 gtaaactcgg cgtgcgcccg gtgtttgatc cgttgcgtta tccggatatc ggccgccagc
     8821 tttctccgga cgcgtgggat gatgtttcgc aggagtaatc acaactatcg atcaactcat
     8881 tctctttttt tgctaaagtc ggcataaatt tcctgcaagg actggatatg ctgattctta
     8941 tttcacctgc gaaaacgctt gattaccaaa gcccgttgac caccacgcgc tatacgctgc
     9001 cggagctgtt agacaattcc cagcagttga tccatgaagc gcggaaactg acgcctccgc
     9061 agattagcac gctgatgcgc atcagcgaca aactggcggg tatcaacgcc gctcgctttc
     9121 atgactggca gccagatttc acgccggaga atgcccgcca ggcgattctg gcgtttaaag
     9181 gtgatgtcta caccggcttg caggccgaaa ctttcagcga agacaatttc gattttgccc
     9241 aacagcattt gcgaatgctt tccggcttgt atggcgtact gcgcccgctc gatttaatgc
     9301 agccttatcg tctggaaatg gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc
     9361 aattctgggg agatatcatc accaacaagc tgaacgaggc gctcgcagca caaggcgata
     9421 atgtggtgat taatctggcg tcagatgagt attttaaatc ggtgaagccg aagaaattga
     9481 atgccgagat tatcaagccg gtgttcctcg atgagaagaa cggcaaattt aagatcatca
     9541 gcttctacgc taagaaagca cgcggtctga tgagtcgttt tattattgaa aatcggctga
     9601 ccaaaccaga gcaactgact ggttttaata gcgaaggtta cttctttgat gaagcttcct
     9661 ccagcaatgg agaactggtg tttaaacgct acgagcagcg ttaagtttgc agtcaatgcc
     9721 ggatgcggcg tgaacgcctt atccggccta caaaagcatg cagattcaat atattgcaga
     9781 attcacgtag gcctgataag acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt
     9841 acccggcatt tgtcatttag cggtgatgtt tgcctggacc atgaccgccg tgatgatcat
     9901 gatgagcttt cttatggtga cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc
     9961 ctcgccattc ataatgttgt ttccaccagc cgtggtcgcg ccagtgaccg ccatcccagt
    10021 aatagccacg attatcacga tcgcctatct gtaattttac tgacgggact aacgtaattt
    10081 ccgcagcctg cgctgccatg ggagcgacca gaaccaggga aagtgcgagt acgatagatt
    10141 gcatcttttt cacaggttac tccttttatc attgcccgtt atgggtcgat atccggagat
    10201 tacgcgatga gaacggcggt tgttattctc cgcaatccta atatctaagt taccgcattt
    10261 attgagaatt tctccttttt ccctgtcgtt tctctccata ttttcttcac aaaaaagccg
    10321 ggctgcataa aagcaaaccc ggcctgattg agataatgaa aagatattac tgatgattca
    10381 tcatcaattt acgcaacgca gcaaaatcgg cgggcagatt atgtgaaagc aagggtaaat
    10441 cagcacgttc tgccagctct tttggcagat ccaacgtttc accgagaatc gcttccacgc
    10501 tctctttaaa tttcgccgga tgcgcggtgc cgaggaacaa gccatattcg cctggattca
    10561 actggtcacg cagcgcacga tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt
    10621 cttttaactc acgcattgtc tgttgcgtgg tttcatcatc cacggctgca taacccagct
    10681 ctttcagttg ccagattttg cggcggaaca actcttccac acgcggccag ttgtttggct
    10741 gactcacgtc catcgcgttg gataacgtcg cctgagtcgc tttgggtgac cactgaccat
    10801 cgtgcaggaa acgtggcacg gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg
    10861 gcagaccgag tgacttcgcc agcagacccg ccgtcaaatc gccgaagttt ccgcttggca
    10921 ccgagacaac cagctggttg cgcgcttcct gcggcagctg cgcaacagct tcaaagtagt
    10981 agcaaatctg cgccagcaac cggctgatgt taatcgagtt agctgagttt aaccccagcg
    11041 ccactttcag ctcttcatca tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat
    11101 cgccgtcgat ggcaacagtt tcgatattgc cgcccaatgt acagaacagt ttttcttgca
    11161 gtggactgat tttgcctcgt ggatagagga taaccacttt cacattcggt aaaccgtaga
    11221 aagcatgagc cactgccgct ccggtatcac cggaggtcgc ggtcagaatg gtcactggct
    11281 tatcgcccgc aatatgggtc agcatttgtg ccataaagcg accgccgaaa tctttaaatg
    11341 ccagcgttgg cccgtggaac aattccagac aaccgacatc gctttcaaca ttggcgaccg
    11401 gagccgggaa ggcaaacgcc gcgcgcacgc gctcttccag gatttcctgc gggatttcat
    11461 caccaataaa cgccgagagg atcttcgcac tgcgggtgac aaaatccagc ttcagcatct
    11521 catcaatttc agtcaggctg aattccggca ggtcatgcgg aaaaaacagc ccctgatttt
    11581 tgcccaaccc ctgggttacg gcttgcgcaa agctgacctg ctcgctgtga tctttcagat
    11641 tgtagagttt catttagttt tccagtactc gtgcgcccgc cgtatccagc cggcaaatat
    11701 gaacaaaacc ttcctgattt tgcaggtagt tcttacccaa ccagtcggca acgcgctggg
    11761 cggtatccgg cttgtcacac agagcgaaca aggtcgggcc ggagccggag ataccgctcg
    11821 ctaccgcgcc gatttccgcg accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt
    11881 cacggtaggg ttctgcgata acatctttca tcagcttcgc ggcaagctca ggctgacggg
    11941 aatagcaggc gtgaatgaag cctgccagat gtcgcccgtg cgcaatgcaa tcctggcggc
    12001 gatactgcgc cggtaaaata gcccgggctt ctgccgtcga gactttaatc cccggatacg
    12061 ccagcaccca cagccactca tcaaaccctg gcacttgctg gctgatgatg tcgttttctt
    12121 cgatcatcaa ctgcatacca ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc
    12181 cggagattcg tccttccagc tcgcccatca aagccagcaa acgagtgtca ttaagcggct
    12241 tgccacagtg ttcattcatc gccatcagcg ccgcgaccac cgaacaggcg ctggagccta
    12301 agcccgagcc gatcggcata ttcttttcca gagtcatcgc cactggaatt tgcttgccaa
    12361 gctcctggca aaaacgctcc cagcactgat aaacgatatt ttcccgtggt tctgacggca
    12421 gcttatcggc aaagcgtccg aggttgttga gactgaatgt ctctgccgcc tcaaccgtga
    12481 ctacatctcc gagcaatgca ccatcaacgg gtgtcaccgc cgccccgagc acatcaaacc
    12541 cgacgctcat attggcactg gaagccgggg cataaacctt aaccatttta gactcctaac
    12601 ttccatgaga gggtgcgtag cagatcggca aagacaccgg cagctgtaac gtcattaccc
    12661 gcaccgtatc cgcgcaggac cagcggcagt ggctgataat agtggctata gaaggccaat
    12721 gcgttttcgc cgtttttcac tttgaacagc ggatcgttgc catccacttc agcaatcttc
    12781 acgcggcaga cgccatctac atcaatattg ccaacatagc gcaaaacttt tccttcatca
    12841 cgggccttcg ccacgcgcgc ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa
    12901 gtcgcaacat cgccttccgc gttaaactct gcgggcagca cagattcgat ttcgatatcc
    12961 gccagttcca gtgatcgtcc tgtttcacgg gcgagaatca acagcttgcg cgccacatcc
    13021 ataccagaaa gatcatctcg cgggtccggt tcggtatagc ccatttctct tgccagcgtg
    13081 gtggcctcgg agaagctcat cccttcgtct aacttaccga agatgaaaga aagcgaacca
    13141 gaaagaatgc cagagaattt catcaattca tcacccgcgt tgagcaggtt ttgcaagttc
    13201 tcaatgaccg gcaaccccgc accgacgttg gtgtcataaa ggaatttacg ccgtgatttt
    13261 tccgccgcgt aacgcagctg atggtagtaa tccatcgacg aggtgttggc ctttttgttc
    13321 ggggtcacca catggaaacc ttcgcgcagg aagtcggcat attgatccgc cactgcctgg
    13381 ctggaggtgc agtcaacaat gaccgggttt agcagatgat attctttcac gaggcgaatt
    13441 aagcgcccga gattaaacgg ctctttggct tgcgccagtt cttcctgcca gttttccagg
    13501 tttaggccat gcacattggt aagcagagcc ttcgagttgg caacgccgca gacacgtaag
    13561 tcgatatgtt tattcttcag ccagctttgt tgacgcttca gttgctccag cagcgcaccg
    13621 ccaacgccac cgacgccaat cacaaatact tcgataacct gatcggtatt gaacagcatc
    13681 tgatgagtaa cgcgcacgcc agtggtcgca tcatcgttat ttaccacgac agagattgag
    13741 cgttcagaag atccctgagc aatggcgaca atgttgatat tggcgcgggc cagcgcggca
    13801 aagaatttcg ccgagatccc acgcaaggtg cgcataccat cacctaccac cgagataatg
    13861 gccagccgtt ccgtcaccgc cagcggctcc agtaagcctt ctttcagttc caggtagaac
    13921 tcttcctgca ttgcccgttc agctcgcaca cagtcgcttt gcggaacgca gaaactgata
    13981 ctgtattcgg aagatgattg cgtaatcagc accacggaaa tacgggcgcg tgacatcgct
    14041 gcaaagacgc gcgccgccat gccgaccatc cctttcattc ccggaccaga aacgctgaac
    14101 attgccatgt tattcagatt ggaaatgccc ttgaccggta attcgtcttc atcacggctg
    14161 gcaccaatga gcgtacctgg tgcttgagga tttccggtat ttttaatcag gcaagggatc
    14221 tggaactggg cgatgggggt aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa
    14281 agctccatcg cttcctggta ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc
    14341 gggtcgcagg tatagacccc gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa
    14401 caggcagcca gcaccgccgc ggagtagtcg gaaccgttgc gtccaagcac caccagttcg
    14461 cctttttcat taccggcggt gaaacctgcc atcagcacca tgtgatcagc cggaatgcga
    14521 cttgccgcaa tacggcgggt ggactcagca atatcgacgg tagattcgag gtaatgcccc
    14581 actgccagta gtttttcgac cggatcgata acggtaacgt tgtgaccgcg cgcttctaat
    14641 acgccggcca taatggcgat cgacattttc tcgccacggc aaatcagcgc agcgttaatg
    14701 ctatccgggc actgccctaa caaactaatg ccatgcagga catgttttat ttgggcaaat
    14761 tcctggtcga cgaaagtttt caattgcgcc agcgggaatc ccggctgggc ggcggcgagt
    14821 cctgtcagaa gttcggcaaa aatacgttcg gcatcgctga tattgggtaa agcatcctgg
    14881 ccgctaatgg ttttttcaat catcgccacc aggtggttgg tgattttggc gggggcagag
    14941 aggacggtgg ccacctgccc ctgcctggca ttgctttcca gaatatcggc aacccgcaga
    15001 aaacgttctg catttgccac tgatgtaccg ccgaacttca acactcgcat ggttgttacc
    15061 tcgttacctt tggtcgaaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt
    15121 tcctgtacgc gtcagcccgc accgttacct gtggtaatgg tgatggtggt ggtaatggtg
    15181 gtgctaatgc gtttcatgga tgttgtgtac tctgtaattt ttatctgtct gtgcgctatg
    15241 cctatattgg ttaaagtatt tagtgaccta agtcaataaa attttaattt actcacggca
    15301 ggtaaccagt tcagaagctg ctgtcagaga ctcttttttt aatccacaca gagacatatt
    15361 gcccattgca gtcagaatga aaagctgaaa aatacttact aaggcgtttt ttatttggtg
    15421 atattttttt caatatcatg cagcaaacgg tgcaacattg ccgtgtctcg ctgctctaaa
    15481 agccccagac gttgttgtaa ccagtcgacc agttttgtgt catctgccac tgccagagtc
    15541 gtcagcaatg ccatgactcg ttcgcgtaaa gcttgcagtt gatgttggtc tgccgttgtg
    15601 tcacttttcg tcggttgttg tattaatgtt gctaattgat agcaatagac catcactgcc
    15661 tgccccagat tgagcgaagg ataatccgcc accatcggca caccagtaag aacgtcagcc
    15721 aacgctaact cttcgttagt cagcccggaa tcttcgcgac caaacaccag cgcggcatgg
    15781 ctcatccatg aagatttttc ctctaacagc ggcaccagtt ctactggcgt tgcgtagtaa
    15841 tgatatttcg cccgactgcg cgcagtggtg gcgacagtga aatcgacatc gtgtaacgat
    15901 tcaaccagtg tcgggaaaac tttaacatta tcaataatat caccagatcc atgtgcgacc
    15961 cagcgagtgg ctggctccag atgtgcctga ctatcgacaa tccgcagttc gctaaacccc
    16021 atcgttttca ttgcccgcgc cgctgcccca atattttctg ctctggcggg tgcgaccaga
    16081 ataatcgtta tacgcatatt gccactcttc ttgaccaaat gaccgcgaac agggtgatca
    16141 ccgtcaactt attacgcggt gcgaatttac aaatccttaa cgtaagtcgc agaaaaagcc
    16201 ctttacttag cttaaaaaag gctaaactat ttcctgactg tactaacggt tgaattgtta
    16261 aaaaatgcta catatccttc tgtttactta ggataatttt ataaaaaata aatctcgaca
    16321 attggattca ccacgtttat tagttgtatg atgcaactag ttggattatt aaaataatgt
    16381 gacgaaagct agcatttaga tacgatgatt tcatcaaact gttaacgtgc tacaattgaa
    16441 cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc ggcccctctt agcctgttat
    16501 gttgctgtta aaatggttag gatgacagcc gtttttgaca ctgtcgggtc ctgagggaaa
    16561 gtacccacga ccaagctaat gatgttgttg acgttgatgg aaagtgcatc aagaacgcaa
    16621 ttacgtactt tagtcatgtt acgccgatca tgttaatttg cagcatgcat caggcaggtc
    16681 agggactttt gtacttcctg tttcgattta gttggcaatt taggtagcaa acatgcagac
    16741 cccgcacatt cttatcgttg aagacgagtt ggtaacacgc aacacgttga aaagtatttt
    16801 cgaagcggaa ggctatgatg ttttcgaagc gacagatggc gcggaaatgc atcagatcct
    16861 ctctgaatat gacatcaacc tggtgatcat ggatatcaat ctgccgggta agaacggtct
    16921 tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg ttgatgttcc tgactggccg
    16981 tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt gcagatgact acatcaccaa
    17041 accgttcaac ccgcgtgaac tgactattcg tgcacgcaac ctgctgtccc gtaccatgaa
    17101 tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc tacaagttca atggttggga
    17161 actggacatc aacagccgtt cgttgatcgg ccctgatggc gagcagtaca agctgccgcg
    17221 cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca ggcaaaattc agtcccgtgc
    17281 tgaactgctg aagaaaatga ccggccgtga gctgaaaccg cacgaccgta ctgtagacgt
    17341 gacgatccgc cgtattcgta aacatttcga atctacgccg gatacgccgg aaatcatcgc
    17401 caccattcac ggtgaaggtt atcgcttctg cggtgatctg gaagattaat cggctttacc
    17461 accgtcaaaa aaacggcgct ttttagcgcc gtttttattt ttcaacctta tttccagata
    17521 cgtaattcat cgtccgttgt aacttcttta ctggctttca ttttcggcag tgaaaacgca
    17581 taccagtcga tattacgggt cacaaacatc atgccggcca gcgccaccac cagcacactg
    17641 gttcccaaca acagcgcgct atcggcagag ttgagcagtc cccacatcac accatccagc
    17701 aacaacagcg cgagggtaaa caacatgctg ttgcgccagc ctttcaatac cgcctgcaaa
    17761 taaataccgt tcattaacgc cccaatcaga ctggcgatta tccatgccac ggtaaaaccg
    17821 gtatgttcag aaagcgccag caagagcaaa taaaacatca ccaatgaaag ccccaccagc
    17881 aaatattgca ttgggtgtaa gcgttgcgcg gtgagcgttt caaaaacaaa gaacgccata
    17941 aaagtcagcg caatcagcag aatggcatac ttagtcgccc ggtcagttaa ttggtattga
    18001 tcggctggcg tcgtcactgc gacgctaaac gcggggaagt tttcccagcc ggtatcattg
    18061 cctgaagcaa aacgctcacc gagattattt gcaaaccagc tgctttgcca ttgcgcctga
    18121 aaacctgact cgctaacttc ccgtttggct ggcaaaaaat cacctaaaaa actgggatgc
    18181 ggccagttgc tggttaaggt catttcgcta ttacgcccgg caggcaccac agaaagatcg
    18241 ccggtaccgc ttaaattcag ggccatattc agcttcaggt tctgcttccg ccagtcccct
    18301 tcgggtaaag ggatatgcac gccctgcccg ccttgctcta acccggtgcc gggttcaatg
    18361 gtcagcgccg ttccgttaac ttcaggcgct ttcaccacac caataccacg cgcatccccg
    18421 acgctaatca caataaatgg cttgccaaag gtgatatttg gcgcgtcgag ttcgctaaga
    18481 cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt gccagacctg accggtataa
    18541 atccctatct tgcgttcttc cacgttctga ttgccatcaa ccatcaatga ctcaggtaac
    18601 caaaaatgga taaaacttcg tttccgctcc acggttttat cctcttcctg taccgtataa
    18661 agctcggtca caggaatagc gataagcggc ccaacgagtt tttgcggccc gctggtactt
    18721 tggcgaatcg catcttccac atcgctacgg taatcggcac gttcgacaat cacctgccga
    18781 atcagcatta tcggaatcaa caacagcaat actgcaccaa acaggggggt cattttccag
    18841 aacagggggg atttcaacat agtcttctcc tttgcaatgg cagcaggata cgaaggctat
    18901 gtgggaagaa tttgaagcta tgtgaagtga cggtgaagtc gaagcgaggc cagcacgcca
    18961 ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg
    19021 aacgccaacc ccagaccgct gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt
    19081 tcaaaaatac gtgacagcgc gtagtcagga ataccactac cggtatccag caccttaagg
    19141 gcgacgtgtt cctgatccac ttcggcgctt agcgttatgc gaccgctctc gggggtaaaa
    19201 tcgatggcgt tatccagtaa attccccagc gcctgctcca gtaacgccgg ttcagcagca
    19261 acattaacct cagtgggcat aacatgtaga gtgatgtttt tttctgccaa ctgcacggtg
    19321 cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa caacagtcag aacgacttcc
    19381 tgacgattct ccagtcttgc ctggcgtagt aacgtttcta ccaatgcctg catacgcgca
    19441 ttttgcgtca gaatgttgtc ggtaaaacga gccaccactt ccggtggcgg accttcgcgt
    19501 aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt ttagctcatg ggttaacgca
    19561 taaacatact gctcaatata gtttttccct tccagcttca cgcgcatact ttccagcgcc
    19621 tgcgcaagct tacgcaactc gctactgccg agttcaggaa gaggaacggg cttattgtca
    19681 gtgacggaat cggcatagcg agtgagccgg gcaatggagc ggttgatcca ccaaaccatg
    19741 cctgcgccaa tcaccagtgc aatccccagc aaaatggcgc tggcccataa aattcgccgc
    19801 tcgctacgct taatgaccgg agccatcgcc gcgttcggtt tgcctacgct caaaacgcca
    19861 ataagccgcg agccgtccat aatcggcgcg gcaacataca tcaccgaact ttcgggatcg
    19921 gcaggatttt gcaacgtgct gcgcgcacca tactgaccac gcaacgttag ccagacgtca
    19981 ttccagcgcg aataatcctg cccaacggct ttatttgccg aatcgaacaa tactttaccc
    20041 tgcgaatcgg tcatatagac atgatattca ttgcgcactt tgttaatgcc accgatattg
    20101 gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg ccagttgccc atgcgttggg
    20161 tccccagaga gcaaatcggg acgcgccagc tccgccagca acgttgcggt atcgattaac
    20221 gtcccctccg ttgctcttcg cacgcccggt ttaacttctt tgacaaaaat agccagtacg
    20281 aaccaggccg ccaccgccac cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc
    20341 attacaggcc cctcaggcta tatcccatgc cgcgatgagt attaatcgga gaaagatcgg
    20401 ggttgatggc gcgcagcttg gcacgcaacg ttttaatgtg ggtatcgacg gtgcgatcgt
    20461 aggtgtcctg cgcatcttcc cacacgctat ccattagttg ctggcgggac cagacgcggc
    20521 ctggcgactt gagtaacgtc ttcagcaata aaaactcata ccgcgtcagc gttaatggcg
    20581 tgtcaaacca gctgatctgc gccgcgggtt cattcaattc aaaatgacca atacggatga
    20641 cgggagacgg cgacgagaac ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca
    20701 cttcgcgggg tgaaaacggt ttagccacat agtcgtcagc gccaatttcc agcccaagca
    20761 ggcgatcgac ctcttcactt cgggccgtca ggaacagtac aggtaacgcc ggatggagcg
    20821 ccagtaactg gcggcacaat tcaaagccgc tgatatcagg caaaccaaca tcgagaatca
    20881 tgacgtcggg aacctgctgg cgagctttat ccagcaccgg caagcctcgc tcaaagacct
    20941 cgacggcaaa accttcctgc tgcaacatgt agaccagcgt gtcggctatc ccttgctcat
    21001 cttccactaa ccagaccgtt tcccgttgca taaatcgcct ctgttattgc cgccacggca
    21061 tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc tacaactttg tcggagtaag
    21121 ccagataagc gagcgcgttg cgtttggcgt cataaaagcg cacgacctgt aacgacttaa
    21181 agaccaggga cgtgcgtttt ttgaatacta cctcgccctg agctttgccg tttttaatac
    21241 gatccgacag ttcaatcggc ccgacttgct gacaagaaat agccgcatcg gaggtatctt
    21301 ccgccagacc caatccccct ttaataccac ctgttttcgc ccggctcaca taacaggtga
    21361 catttttcac atcgggatcg tcaaaggctt ccacaacaat tttgtgatcc gggccgatca
    21421 ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg agccaatggc cccagcatta
    21481 tcaggcttaa agacaggatc aaatgcttgt atttcatatt gttaccattt ttatctatta
    21541 cacgagcaat tagttcgtca cggtaaataa catattgatt aagaacaaaa tctcaatact
    21601 tttatttccg tcaagaccta aaacatactc tactaagaaa aaaacactga atgctaaaac
    21661 agcaaaaaat gctattatcc aattacctga tgtcaggtgc tcgttgttga aaggatgagg
    21721 atattttatg gatcaggccg gcattattcg cgacctttta atctggctgg aaggtcatct
    21781 ggatcagccc ctgtcgctcg acaatgtagc ggcgaaagca ggttattcca agtggcactt
    21841 acagagaatg tttaaagatg tcactggcca tgctattggc gcgtatattc gtgctcgtcg
    21901 tttgtcgaaa tcggcggtcg cactacgcct gactgcgcgt ccgattctgg acatcgcgct
    21961 gcaataccgc ttcgactctc aacagacatt tacccgcgca ttcaagaagc agtttgccca
    22021 gactcctgca ctttaccgcc gttctcctga atggagcgcc tttggtattc gcccgccgct
    22081 gcgtctgggt gaattcacta tgccagagca taaatttgtc accctggaag atacgccgct
    22141 gattggtgtt acccagagct actcctgttc gctggagcaa atctctgatt tccgccatga
    22201 aatgcgttat cagttctggc acgattttct cggcaacgcg ccgaccattc cgccagtgct
    22261 ctacggcctg aatgaaacgc gtccgagtca ggataaagac gacgaacaag aggtattcta
    22321 taccaccgcg ttagcccagg atcaggcaga tggctatgta ctgacggggc atccggtgat
    22381 gctgcagggc ggcgaatatg tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga
    22441 gtttatcctg acggtatacg gaacgtgcat gccaatgctc aacctgacgc gccgtaaagg
    22501 tcaggatatt gagcgatact acccggcaga agacgccaaa gcgggagatc gcccaattaa
    22561 tctgcgctgt gaactgctga ttcctatccg ccgttaacgc tgcagctcat ctaatgcagg
    22621 ggcgtccaga tgcgagatgt cccctgcggt ttcaacgacc cagccagacg ccagccacag
    22681 gctttcctga taatccacgc gcgaaataga acagttacgc agacgtaagc ggcgctctgc
    22741 ccatgctggt aatccgagaa tcgtactcac caggcatccc agtgcaatac catgactcac
    22801 cagcagcggt cggcttccct gcggtaagtc ccggcaagat tccagtgccg cattgacgcg
    22861 atcgctgagc tcctgcattg actcgccttc aggaatacgc ccgtcaacgg tgccattgac
    22921 cagctgccga cgccagttct cttcttcttc ggtcagagaa tcgatatgtc ttttttccag
    22981 cacacccatg tttaattcac gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg
    23041 ggcgatgatt tccgccgtac gccgggtgcg ccctaaatcg ctgctgatga tatgcgtaat
    23101 gccaagctct ttggcacgcg tcgccacctg catcgcctgt tgctcacctt tggcggtcag
    23161 cgggctgtca gactggccct gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg
    23221 gactaggtat acctgtaaca tacttttttt ccgtatactg ctgtaacgtt aattttctga
    23281 gtaatgctga ttatgcacca agttgtctgt gcgaccacca atcccgctaa aattcaggcc
    23341 attctgcagg catttcacga gatcttcggc gaaggatcct gccatattgc atccgttgcc
    23401 gtcgagagcg gtgtaccgga acaacccttt ggcagtgagg aaacgcgcgc tggcgcacga
    23461 aatcgggtag caaatgcccg ccgtttactt ccagaggctg atttttgggt ggcgattgaa
    23521 gctggcattg atggcgacag cactttcagt tgggtggtga ttgaaaacgc cagccagcgc
    23581 ggcgaggcgc gttcggctac tttgccgttg cctgcggtga ttctggaaaa agtgcgtgaa
    23641 ggtgaagcgc ttggtcccgt gatgtcgcgt tataccggta ttgatgagat tggtcgtaag
    23701 gaaggggcga tcggcgtgtt taccgccggg aaactcactc gcgccagcgt ctatcaccag
    23761 gcggtgatcc tggcgctgag tccgtttcat aatgccgtgt atcaggcatt gtaggacgga
    23821 taaggcgttc acgccgcatc cggcattcgg tgcacgatgc ctgatgcgac gctgccgcgt
    23881 cttatcaggc ctacaaaatc aatcgctttt cagcaacacc tcttccagcc actggcgcag
    23941 ctcaacgggc gcggctttca ggctgttaga tccacgcgta atcgtcgcga tgcccgcgcc
    24001 gagttcattt tttaactcac gctggctcat ttcgccgcgc aacagctctt cgacaatacg
    24061 cacgcgagtc cccaacgctt cgcgctcatc tggcgtcagc atcaggttta acaacggtaa
    24121 atggagatcg ttttggtagg cattcttaag caggtcgaca aaacgtaacc actcctggtg
    24181 acgctgttct gccatcgctg ctgaataggg tgattgttgg gccataatat gtcgccattg
    24241 ttagcggggg aagcaaaatg cctcccccgg ttgtactcgc taaagagtac aatagcatat
    24301 cataaacgtg cggatcagta acgacgtccc cattccgtgg cgctcatcaa cgtcggttta
    24361 tcccccatga aatagcggta gtaagcgtca taagccagca cgttcttcac ataaccgcgc
    24421 gtctcggaga acggaatact ctcgacaaat gccacagcgt cgatacgccc ggcgctgttt
    24481 ccaagccagg ttcgcacccg tccaggtccg gcgttataag ctgctgagga gaaaatacgg
    24541 ttattgccaa actgctgata aacatattgc aggtaactgg tgccaatgtt gatattcgtt
    24601 tccggatcca gcaattgccc agggctgcta taaccaggaa tagagaacat cttcaccgta
    24661 tgggtcgctg taccaggcat aatctgcatc aggccgctgg cccctaccgg tgatttcact
    24721 ttcggattcc aggcgctctc ctgacgggca atcgccatcg catagctttg cgggatctct
    24781 ttcccactgg tgtaacgttt gaaaagatcg ttgtaagcca gcgggaatcg ctcttccaga
    24841 tgatcccaaa gcttcccggc gatcgttgcc tgaacgctaa gatcccacca ttggttattg
    24901 aaagcatacc gagccagttg agcctgctct gtttttgact tgctcttcac cagattggcc
    24961 cactcgctac gcgcggtgtt atcgagattc cagtacatca actcgcgcac gcgcgccatc
    25021 tccggcccct gagtcagggc gctgtcaaca ttctgcggcg ctttatcaat cttcagctca
    25081 tactcttctc cgatgcgttg tgctgcaacc atcgggtaga aaccacgctg ttgcatgagt
    25141 tgatgcaaaa tctctttcgc ttcagcttca cgtccgcgtt ccagcaataa atccgcctgc
    25201 cagtaacgcc attcatcttt ctcttttgct tccatcggca gacgcgccag ccaggtattc
    25261 aggccgcgac gatcgccggt gccaagtgcc attcgcacac ggcgttcaat caacgacgtg
    25321 gattgcgagc gcataatggc gtcatcgcgc catttcgcct gctcgtcggt gacatcgttg
    25381 cccatcaaac gccaggcgac gatatcgcgc agctcctgaa tctgatcttc attaagctgc
    25441 tgcgcctggg caagcgacgg gatcatcagc cgtgcatttt cagcatcctg ccgcgccaca
    25501 ctggcaaacg ccaccgctgc catttgacgg gtaaaatcgg tcgctccggt tgtacgcgcg
    25561 aaggtcagta ccgtattagg gttgttcgcc agtgaaatga ttgccgaggc gatagtctgg
    25621 taatcggcag gcatctgccc tgccagcact gttaccaggc cagtgttacc cgctttcatc
    25681 gccagacgga tacgctctaa atacgccagc ggatcttgtt tacctgacgc acgccagacg
    25741 ctaaataact tgtcacaggc gttaggctgg ctcttgccgg ttagccacag ctctttcgcc
    25801 ccttgccagg cttcttcact ctgcccggtg ttccatttcg catagtagta attacattgc
    25861 gcttcggtag ttccgggctt ttccgggcta aaggctaaca agccacgcca gtcttcacgc
    25921 cgcgccagtt cattgacgaa acgagattgc agcgtgcgag cgggaggaag cgtggggtta
    25981 gcgcgaacaa agttagtgac cgtcaccgcc ggttgattca tcagatcatc ggtgatctgg
    26041 cggtattcca ggtagggata aagcggataa tccttcagtc caggcatcat ttgttcgacc
    26101 acatccattt gtcgattatc ccaggcctgc ttgatttgcg cgtaacggct acgctgctca
    26161 tccagtgagt cggctcgcgc cacgctgctg accgtcagca gacagacacc ggcagccaac
    26221 agccgccagg taacttgttt ggctttttcc acaagcgcat cctctaagtg taaatacatc
    26281 aatgcagcgt catgccgcgt aatagattta tgctaaccag tcattgccgt ttacgccacg
    26341 ttacggacac ttttttactt ttactgcgaa ggtgatcgac caactgggat taggcgggga
    26401 aaaaggctac acttcgcctt tgaaaactca tcatcacgat aaacatagag gcgaagtcca
    26461 acgtggctca attcgtttat accatgcatc gtgtcggcaa agttgttccg ccgaaacgtc
    26521 atattttgaa aaacatctct ctgagtttct tccctggggc aaaaattggt gtcctgggtc
    26581 tgaacggcgc gggtaagtct accctgctgc gcattatggc gggcattgat aaagacatcg
    26641 aaggtgaagc gcgtccgcag ccagacatca agattggtta cctgccgcag gaaccgcagc
    26701 tgaacccgga acacaccgtg cgtgagtcca ttgaagaagc ggtttctgaa gtggttaacg
    26761 ccctgaaacg cctggatgaa gtgtatgcgc tgtacgccga tccggatgcc gattttgaca
    26821 agctggccgc tgaacaaggc cgtctggaag agatcattca ggctcacgac ggtcataacc
    26881 tgaacgtaca gctggagcgt gcggcggatg cgctacgtct gccggactgg gacgcgaaaa
    26941 tcgctaacct ctccggtggt gagcgtcgtc gcgtagcgtt gtgccgcctg ctgctggaaa
    27001 aaccagacat gctgctgctc gacgaaccga ccaaccacct ggatgccgaa tccgtggcct
    27061 ggctggaacg cttcctgcac gacttcgagg gcaccgtggt ggcgattacc cacgaccgtt
    27121 acttcctcga taacgttgca ggctggatcc tcgaacttga ccgcggtgaa ggtattccgt
    27181 gggaaggcaa ctactcctcc tggctggagc agaaagatca gcgcctggcg caggaagctt
    27241 cacaagaagc ggcgcgtcgt aagtcgatcg agaaagagct ggagtgggta cgtcagggaa
    27301 ctaaaggccg ccagtcgaaa ggtaaagcac gtctggcacg ctttgaagag ctgaacagca
    27361 ccgaatatca gaaacgtaac gaaaccaacg aactgtttat tccacctgga ccgcgtctgg
    27421 gcgataaagt gctggaagtc agcaacctgc gtaaatccta cggtgatcgc ctgctgattg
    27481 atgacctgag cttctcgatc ccgaaagggg caatcgtcgg gatcatcggt ccgaacggcg
    27541 cgggtaaatc gaccctgttc cgtatgatct ctggtcagga acagccggac agcggcacca
    27601 tcactttagg tgaaacggtg aaactggcat cggttgatca gttccgtgac tcaatggata
    27661 acagcaaaac cgtttgggaa gaagtttccg gcgggctgga tattatgaag atcggcaaca
    27721 ccgagatgcc aagccgcgcc tacgttggcc gctttaactt taaaggggtt gatcagggta
    27781 aacgcgttgg tgaactttcc ggtggtgagc gcggtcgtct gcatctggcg aagctgctgc
    27841 aggttggcgg caacatgctg ctgctcgacg aaccaaccaa cgacctggat atcgaaaccc
    27901 tgcgcgcgct ggaaaacgcc ctgctggagt tcccgggctg tgcgatggtt atctcgcacg
    27961 accgttggtt cctcgaccgt atcgccacgc acatcctgga ctaccaggat gaaggtaaag
    28021 ttgagttctt cgaaggtaac tttactgagt acgaagagta caagaaacgc acgctgggcg
    28081 cagacgcact ggagccgaag cgtatcaagt acaagcgtat tgcgaagtaa tgcgtaaaat
    28141 gccggatgcg gcgtgaacgc cttatccggc ctacaaacca agaaatttca atacgttgtg
    28201 aatctccagg cctgataaga cgcggtaagc gtcgcatcag gcattgagca ccgaatgccg
    28261 gatacggcgt gaacgccttt tccggcctac aacagcctta gtatccgcaa tttcgtagcg
    28321 ggtcacccgt gcaggttttt tcaaaccttg tttaatctcg acagcctctt ccagagaggt
    28381 tttcaactca tcaaaaaact tattttatac ctcgcctttg agccgtttca tcgcggttat
    28441 ctctgctccc ccatcatctc ccgcaccagc tccacgcagc gcaggaaacg gctgtcataa
    28501 tcgtcctctt caacccgcac gaattcgata ttgttctctt ccagcatctc caccagcaag
    28561 ttctggaact ctttgcgatc caccgaactg ccgaggctgc gtaaaccatc cgccacccac
    28621 ggcgtgttgt tctccagcag gatcaccaga tcgaaacggt attcatcaat caacgcctgt
    28681 acgaacggat gctcacgccc ttcgtacttt ttgcagaacg cctgggtagt gacaaaatca
    28741 gtgtcaataa acgccacttt attggcatat ttcactgcaa aatcaatgta ttgtgcgtgg
    28801 cccagcgcga ttttatcgta atcggaatac tgcaatgcga tctcatcacc gccgaggtgt
    28861 gaaaagacat aatcgcgacc atattcccac gcactggtgg tattgaagat attggcaagt
    28921 ttgtttacca gggtggattt accgctcgac tcgccaccaa ggatcgccac ggtacgtaca
    28981 aagaacggct tcacttccgt aggaatatat tcccagtagc ggaacgggtt ttcgcggatc
    29041 tgcgcaccgc tgatactcat aaaggtacgt ttcggatcga ccagcaccgt atcgatcccc
    29101 agatgttcca tatactgtgg cgcatcggct tcttccgagg tgtagatcag gtccggctga
    29161 atcccctttt cagccataaa ctttttgatg ccgttgctcc acacatccca gccgtgcgga
    29221 tacggctcca tgccctcttc gttgaaagca tgaatgcgaa tatttttctg atatttaaaa
    29281 gtttgtaata accagcgcag acgatccggc accgtgggct gctgcgacat ggcgctgtct
    29341 tcaaacaacg cgcggtcgcg ggtatcgtca aaacccataa tgatatgcag ctcgtcaacc
    29401 tggctacagg cgcgctggat aaggtagata tgtccggtat gcagcgggta gaacttaccg
    29461 aatacgacac caatggtttt cttctgtcgg ggaaattcaa gccccaaaaa acggtgcaac
    29521 gcctccagct tttgcgcgct ggggctttta attttggcat tcagtaactg gcttaaatac
    29581 cctttggtca taccgctggc atcagctacc tgctgtagcg tgcagccctg ttgcttgatg
    29641 gcagttttca ggtaatcaaa tgacgacata tctccctccg tatctctcat tataagtcgt
    29701 cgaacacgct aagcgcgtcg gagagttttt taacgccaaa aatctgcatc ccttccggcg
    29761 cttttttcgg tacgttagcc gccggaacaa tcgcccggcg aaaaccgtgt ttcgccgctt
    29821 ctgaaattcg ttcctgaccg ctgggcaccg ggcggatctc ccctgccagc ccgacttcac
    29881 caaacaccac cagatcctgt ggcagcggtc tgtcacgcag gctggaaacc atcgccagca
    29941 gtaacgctaa gtcggcactg gtttcggtta ccttcacgcc gccgaccacg ttcacaaaca
    30001 catcctgatc ggccatttgc agaccaccgt ggcggtgcaa cacagccagc aggattgcca
    30061 gacggttttg ttccagcccc actgccacgc ggcgtgggtt cgccatcatc gagtgatcga
    30121 ccagcgcctg aatctccacc agcagtggac gcgttccttc ccataccacc atcactgagc
    30181 taccggaggt cacttcatcg ccgcgactta agaaaattgc cgaagggttg ctgacttcac
    30241 gcagcccctg ttcggtcatc gcgaagacgc ccagctcatt caccgcgccg aagcggtttt
    30301 tatggctgcg taaggtgcga aaacgggagt cggcatcgcc atccaaaagc accgaacagt
    30361 cgatacagtg ttccagcact ttagggcccg ccagcgagcc atctttggtt acgtgtccaa
    30421 ccatgacaat cgccacaccg cgcattttgg cgaagcgcgt cagataagcc gctgtttcac
    30481 gcacctgcgc cacgctgcct ggcgatgatt gtacatccgc catatgcatc acctggatcg
    30541 agtcaattac catcagcttc ggttgctctt cttcggcaat caggcagatc tgttcgatgc
    30601 tggtttccga caacatattg agattgtcag tcggcaggcc aaggcgatga gcgcgcattg
    30661 ccacctgttg cagcgactct tcgccagtga catacagcgt tttcatctgc tgcgccagtt
    30721 tgcacagcgt ttgcaatagc aatgtggatt tcccggcacc tgggttaccg ccaatcagaa
    30781 tggcgcttcc cggcaccacc ccgccgccta gtacgcggtc gaactcttta aatccggtgg
    30841 aaaaacgcgg cagctcttca aggctgatat cggagagttt ctggactttt gccaccccgg
    30901 cgctaccggc atagccgctg agacgctcgt cacgcgccac cgttggcgac gcagcaagac
    30961 gcacctcggt gatggtgttc caggcatgac aggcactgca ctgcccctgc cagcgcggat
    31021 aatcggcccc gcattcatta caaacaaagg cgcgttttgg agcttttgcc acggtgtacc
    31081 tcgttaatgc tgtgccgccc gcaggatggc gggcgagcaa ttacttctga ttcaggctgc
    31141 ctgagaggat gcagaatacc cccatcaggt cagcgtgacg gatggtgact tccgcctttt
    31201 cattcacttt tggcttggca tggtaggcaa tccccagccc tgccgctttg atcatcggca
    31261 ggtcattggc tccatcgcca atcgccacgg tctgcgccag cgggatttca tactcctgcg
    31321 cgaggcgagt cagagttttc gctttgtact gcgcgtctac gatgtcgccg atcacattgc
    31381 cggtaaattt accgtccatg atctccagtt cattggctac cacggctgtc aggcgcagct
    31441 tgtcgcgcag gtattcagca aagaaagtaa agccgccgga ggcaatcgcc actttccagc
    31501 ccagcgtttc cagcttgagc accagttgcg ttaagcctgg catcagcggc agattttcac
    31561 gcacctgttg cagaatattg gcgtcagcgc ctttcagcgt cgccacgcgg ctgcgcaggc
    31621 tggcggtaaa atcgagttcg ccgcgcatcg cccgttcggt tacttccgcc accatctcgc
    31681 ccgttccggc cagtttggca atttcatcaa tacattcaat ctggatggcg gtggaatcca
    31741 tgtccatcac cagcaaaccc ggcgtgcgca ggtgcgggat tttccccagc ggcgcgacat
    31801 ccagttgcgc ttcatgggcc aggcgcgtag cccgtgcggt gagtgaacct gccagacgaa
    31861 tcacctgata atcttccacg caccaggcgg caacaatcac catcgccgca cccagtttgc
    31921 tctggtattg ggtcagacgc tgtttatcca gcccacgacc atacagcagc cagccgctac
    31981 gacctgcgtg gtaatccagt ggcatcactt catcaccact taatgaaaga ggcagacccg
    32041 gccataaaga gacatcttca ggcaggtcgc accaggtaat gttaggcatt aaggctcctg
    32101 taaaatcgtt cgaagcaggg aaaataacgc atgaggctac cttgtatcca ttgcttctgg
    32161 caacattaag tctcaaattt tcaaagggtg gaagatggct cgcacaaaac tgaaattccg
    32221 gctgcatcgg gcagtgattg tcctgttctg tcttgccttg ttagtggcac tgatgcaggg
    32281 ggcgtcatgg tttagtcaaa accaccagcg acagcgtaat ccacagctgg aagaactggc
    32341 ccgcaccctg gcgcgtcagg tgacgctgaa cgttgcaccg ctgatgcgta ccgactcacc
    32401 agatgaaaaa cgcattcagg cgatcctcga tcagttaacg gatgaaagcc gcatcctcga
    32461 cgcgggtgtg tatgacgaac aaggcgatct tatcgcccgt tcaggcgaaa gcgtcgaagt
    32521 gcgcgaccgg ctggcgctcg acggtaaaaa agcaggcggc tattttaacc agcagattgt
    32581 cgagccaatt gcgggtaaaa acggaccgct cggctatctg cgcctgacac tcgacaccca
    32641 tacgctcgcc accgaagccc aacaggtgga taacaccact aacattttac gcctgatgtt
    32701 gctgctctca ctggcaatcg gcgtagtgct gacccgcacg ctgctacagg gtaaacgcac
    32761 ccgctggcag caatcgccct tcctgttaac cgccagcaaa ccggtgccgg aagaggaaga
    32821 aagcgagaaa aaagagtgac ccattactac aagaaaggaa atcgttatgt ccacattacg
    32881 cctgctcatc tctgactctt acgacccgtg gtttaacctg gcggtggaag agtgtatttt
    32941 tcgccaaatg cccgccacgc agcgcgttct gtttctctgg cgcaatgccg acacggtagt
    33001 gattggtcgc gcgcagaacc cgtggaaaga gtgtaatacc cggcggatgg aagaagataa
    33061 cgtccgcctg gcgcggcgca gtagcggtgg cggcgcggtg ttccacgatc tcggcaatac
    33121 ctgctttacc tttatggctg gcaagccgga gtacgataaa accatctcta cgtcgattgt
    33181 gctcaatgcg ctgaacgcgc tcggcgtcag cgccgaagcg tccggacgta acgatctggt
    33241 ggtgaaaaca gtcgaaggcg accgcaaagt ctcaggctcg gcctatcgcg aaaccaaaga
    33301 tcgtggcttc caccacggca ctttgctgct caatgccgac cttagccgcc tggcaaacta
    33361 tctcaatccg gataaaaaga aactggcggc gaaaggcatt acctcagtgc gttcccgcgt
    33421 gaccaacctc accgagctgc tgccggggat cacccatgag caggtttgcg aggccataac
    33481 caaggccttt ttcgcccatt atggcgagcg tgtagaagcg gaaatcatct ccccggacaa
    33541 aacgccagac ttgccaaact tcgccgaaac ctttgcccgt cagagtagct gggaatggaa
    33601 cttcggtcag gctccggcat tctcgcatct gctggatgaa cgctttagct ggggcggcgt
    33661 ggaactgcat ttcgacgttg aaaaaggcca tatcacccgc gcccaggtgt ttaccgacag
    33721 cctcaacccc gcgccgctgg aagccctcgc cgggcgactg caaggctgcc tgtaccgcgc
    33781 ggatatgctg caacaagagt gcgaagcgct gttggttgac ttcccggacc aggaaaaaga
    33841 gctacgggag ttgtcgacgt ggatagcggg ggcggtaagg taactcgctg attaaaatac
    33901 caaaggaagg gaatatgatc gatctggaaa accaggagcg ggagattatc aatttaatgt
    33961 taagccagcg tatttcctgg ctagccgccg tgcgtatcag gcataaatta tcgcttgctg
    34021 aagtaagtaa aatgttaggt atctcgatta attctctaaa atagatcgaa aagacggaac
    34081 ggtttagctc aaacatcaaa agcaaaatgg cagaaattta tggttgtcca ccagagttgc
    34141 tgatatgccc ttcctggatg acggctgaac ataaatgaat agattcgtga atagatactt
    34201 gaatagattt tattttctat ccatatgaaa aataatagta aatctataaa tcagttgcta
    34261 ttgatgaata gatactaaaa agccggagca gtctcccact ccggcttcac acgacaatcg
    34321 cctttcagcg aaacacaatt actctttatc gcccagcaga acggattcca gtgcgatttt
    34381 gatcatgtcg ttgaaggtgg tctgacgctc agcggcagtg gtctgctcgt gagtgcggat
    34441 gtggtcagac acggtacaga tggtcagggc tttcgcgcca aattctgcag cgacgccgta
    34501 gataccagcc gcttccattt ccacgccgag gatgccgtat ttttccatca cgtcgaacat
    34561 ttcgccgtcc ggagagtaga acaggtcagc ggagaacagg ttaccaacgc gagcatcaat
    34621 gcccagtgct ttagccgcgt ctaccgcgtt acgcaccatg tcaaagtcag cgatagcggc
    34681 aaagtcatga tctttaaaac ggatgcggtt aactttggaa tcggtgcagg cacccatacc
    34741 gataacgacg tcgcgcagtt ttacgtgcgg cagaactgcg ccacaggaac ccacgcggat
    34801 aattttcttc acgccgaaat cggtgatcag ttctttggtg tagatggagc aggacgggat
    34861 acccataccg tgacccatta cggaaatttt gcggcctttg taagtaccgg tgaagcccag
    34921 cataccgcga acgttgttca cttcacgggc atcttcaagg aaagtttcag caatatactt
    34981 cgcacgcagc gggtcgcctg gcatcaaaac tacgtcagcg aaatcgccca tttctgcatt
    35041 aatgtgtggg gtagccattg ttttatcctt atcaacatgt tttcaccctc tccctgaggg
    35101 agagggccgg ggtgagggca tcaagccgca cccattatca gaacatggct ttgccatatt
    35161 ccatatcaga agtaccaaaa tattttgcca gagtctggcc gatatccgcg aaggtttcac
    35221 ggtgacccag tgagcccggt tttactttcg ggccgtaaac cagtaccgga atgtgttcac
    35281 gcgtgtggtc agtaccggtc caggtcggat cacaaccgtg gtcagcagtg aggatcagga
    35341 tgtcgtcatc gcgcagcaga gacatcagct ccggcagacg gcggtcgaac agctccagac
    35401 ccgcagcata accggcgacg tcgcgacggt ggccccagga agagtcgaag tcaacgaagt
    35461 tggtgaagac gatggtgtta tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca
    35521 gcgcgtccag gccagttgct ttcactttct tggtgatacc gcagttggcg tagatgtctg
    35581 caattttacc gacagaaacc acctgaccgt gtttttcatc aaccagtttc tgcagcacgg
    35641 tcggtgctgg cggctcaaca gccaggtcgt gacggttacc ggtacgctgg aagttaccgg
    35701 ctttgtcacc gataaacgga cgagcgataa cacgaccgat gttgtagccg ccgttggtca
    35761 gctcttcacg ggcgatttcg cacagttcgt agagtttatc caggccaaag gtttcttcgt
    35821 ggcaggcaat ctggaacaca gagtcagcgg aggtatagaa aatcggcttg ccggttttca
    35881 tgtgctcttc gcccagctga tccagaatga ccgtaccgga agagtggcag ttaccgaggt
    35941 aacccggcag attagcgcgt tcaaccagtt tatccagcag ctcttgcggg aagctgtttt
    36001 cgtgatcgga gaaatatccc cactcaaata gaaccgggac accggcgatt tcccagtgac
    36061 cagacggggt atctttaccg gatgacattt cgtgcgccca tgcgtacgcg ccgataactt
    36121 cagcgttgcc gtccattccc gccggaatga aaccggtaga accttcgtgt gctttcgcca
    36181 gccccagacg ggtcagattt ggcagattga gcggaccttt acgaccgtta tcagcttcgc
    36241 ctttggcaca agcttctgcg atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt
    36301 ctgcatcttc tgtagcgccg atgccgaatg agtccagcac cataataaat gcacgtttca
    36361 tatgttctcc gtactcagtg cttcaaattt aaatgatcag atcagtatac cgttattcgc
    36421 tgatacggcg atagacagtt ggtgtgcttt ccggtgcttt atcggcaagt ttaattgccg
    36481 ctttcaccgc tttcgccgct tcctgccagc tgttttcgtc tttcgcgtgg ataaccgcca
    36541 gcggacgctg accgtctacc tggtcgccca aacgcgccat atcagtaaag ccgacgctgt
    36601 aatcaatggt gtcagatgcc tgacgacgac cgccgcccat tgcaaccact gccatcccca
    36661 gcgcgcgggt atccatttca ctgacaaaac cttcggtatc agcatagact gctttcgtca
    36721 gcatcgctgt cggcagatac ttcgcgtagt tctcaacgaa gtcggttggg cctttttgtg
    36781 ccgctaccat acgaccaaag acttctgccg ctttaccgtt gtccagcact gcttgcaatt
    36841 tcgcgcgcgc ttcggcgtca tctttcgcca gtttgccgga gataagcatc tccacgcaca
    36901 gcgccatcgt gacatcaaac agacgcgggt tacgatactc acccgtcagg aactgcaccg
    36961 cttcacgaac ttcaaccgcg ttacctgcac tggaggccag tacctgattc atgtcggtga
    37021 gcagcgcggt ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct tcggcaaggg
    37081 cttcagagag ttcgtaggtc ggcataaacg cgccgctacc cactttcacg tccatcacca
    37141 gcgcgtccag accttccgcc agtttcttcg ccagaataga ggcggtgatc agcgggatgg
    37201 agtccacggt tgcggtaata tcacgggtcg cgtagaaacg tttatccgcc ggagccagtg
    37261 agctggtctg accgataatc gccacgccga cgtctttaat aatttcgcgg aaacggttgt
    37321 catccgggaa aatgtcgaag ccagggatgg attccagttt gtcgagcgta ccgccagtat
    37381 gaccgaggcc gcgaccagag atcatcggaa tatagccgcc gcaggctgcg accatcggcc
    37441 ccaacatcag cgaagtcaca tcgccgacgc caccagtgga atgtttatca acaatcgggc
    37501 cattcagatg caggcttttc cagtcgagaa cggttcctga atctcgcatc gccatggtca
    37561 gcgagacacg ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa
    37621 tctgcccttc ggagatagtg ttgtcgcgaa tgccgttgat aaagaaacga atttcttcat
    37681 cgctcagtgc atgaccatca cgttttttac gaataatttc ttgtgcgaga aacaaggtaa
    37741 cctcccgagg aaaagagtat gaggcggctt ttgccggatg cgtctacgcc ttatccggcc
    37801 tacgggtaga tacgtctcgt aggcctgata agcgcagcgc atcaggcgta aagcatctta
    37861 cttagtagct gctggcgctc ttaccgtcac cgtggcccag cgctttcaac aggcttgcca
    37921 gcaggctgga agcaccaaag cggtagtgac gcgcatctgc ccagtcagca ccgaacagtt
    37981 catctgcgat ggcgagatat ttctgcgcat cttccgcagt acgcacgccg cccgctggtt
    38041 tgaaaccaac ggttttttct acgcccatat cacggatcac ttccatcatg atgcgcgcgc
    38101 tttccggcgt cgcgttcaca gccactttac cggtagaggt tttgatgaag tccgcacccg
    38161 ctttgatgga gatttcagac gctttacgga tcagcgcttc gtctttcagt tcgccggttt
    38221 cgatgatcac tttcagcagt acattcgctg ccgcgcaagc ctctttacag gctttcacca
    38281 ggtcaaaacc aacctgctcg ttacccgcca tcagcgcgcg gtacgggaac accacgtcaa
    38341 cttcatcggc accgtaggcg attgccgcac gggtttctgc cagcgcgatt tcgatgtcat
    38401 cgttaccgtg tgggaagttg gttaccgtag caatacggat ttccggggtg ccctgctctt
    38461 tcagtgtttt gcgagcaatc gggataaagc gaggatagat acagatagcg gcggtattgc
    38521 cgaccggggt tttggcctga tgacacagag caattacttt ctcgtcggtg tcgtcgtcat
    38581 tcagggtggt caggtccatc aatttcagtg cacgcaggct gcttgctttc agatcagtca
    38641 tttcattctc ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta gtattctaac
    38701 atctactccg caacacactt cgatatacat cacaattaag gaaatctact tacaagtttg
    38761 catcactgta atgcgatctg gttcaaataa ttcactttca aatgaatgcg tcagtggtgg
    38821 caaacgcatc aggatcaaag tgaacatcac gaaacttctt acaatggcgc atcaccttcg
    38881 gcataaaagg aaaagaagat gcccacttct catgaaaatg cactgcaaca acgttgccag
    38941 caaattgtca ccagcccagt gcttagcccg gagcagaagc gccattttct ggcactggaa
    39001 gcagaaaaca atctgcctta cccacagctt cctgccgaag cccgccgcgc gctggatgaa
    39061 ggtgtaatct gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc
    39121 gattacgccc gttttctggc gaacggttcc gaatggctgg agctggaagg cgcgaaagat
    39181 cttgatgacg cactctctct gctgaccatt ctttaccacc acgtaccgtc ggtcacatcg
    39241 atgccggtct acctggggca actggatgcg ttgttgcaac cgtatgttag aattctaaca
    39301 caagacgaga tcgatattcg aataaaacgt ttctggagtt acctcgacag aaccctgcca
    39361 gacgccttta tgcacgccaa tatcggcccg tctgattcgc ccattacccg cgcgatctta
    39421 cgtgcagacg cagagttgaa gcaggtttcc ccgaacctga cctttatcta cgatcctgaa
    39481 atcacccctg atgacctgct gctggaagtg gcgaagaaca tctgtgaatg tagcaaaccg
    39541 cacatcgcca acggtccggt gcatgataaa attttcacaa aagggggcta cgggattgtg
    39601 agctgttaca actcactgcc gctggcgggt ggtggcagca cgctggtacg ccttaacctg
    39661 aaagccattg ccgagcgcag cgaatcgctg gatgacttct ttacgcgtac tctaccgcac
    39721 tactgccagc agcagatcgc catcatcgat gcgcggtgtg aattcctcta tcaacaatca
    39781 cacttctttg agaatagctt cctggtgaaa gaagggctga ttaaccctga acgttttgtg
    39841 ccaatgtttg gcatgtatgg gctggcggaa gcggttaact tgctgtgtga gaaagaaggg
    39901 attgccgcgc gctacggtaa agaagccgcc gcaaatgaag tgggttatcg catcagcgcg
    39961 caactggcgg agtttgtcgc caataccccc gtgaaatatg gctggcaaaa acgcgccatg
    40021 ttacacgcac agtcggggat cagttccgat atcggcacca cgccgggcgc gcgtttgccg
    40081 tatggcgatg agccagatcc gatcacccat ctgcaaactg tcgccccgca tcatgcttat
    40141 tattattccg gcatcagcga cattctgacg ctcgacgaaa ccatcaaacg taacccgcag
    40201 gcactggtac agctttgcct cggtgccttt aaagccggaa tgcgtgaatt taccgccaat
    40261 gtcagcggta acgatctggt tcgcgttacc ggttatatgg tgcgtttgtc ggatttagaa
    40321 aaatatcgcg ccgaaggttc acgcaccaac accacatggc tgggcgaaga agccgcacgc
    40381 aacactcgta ttctggaacg ccagccgcgc gtgataagcc atgaacagca gatgcgcttt
    40441 agtcagtaag attatcccct tctcctgcgt tgacgggcca ggcagtcgtc tggctctgtt
    40501 tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat ccgtggacga tgggacgttg
    40561 caatgactgt ggggagtgcg tgccacagtg tccgcatcag gcgttgcaga ttgttgacgg
    40621 caaagtggtg tggaacgctg tggtctgcga gcaatgtgat acctgcctga agaggtgtcc
    40681 gcaacatgcc acgccaatgg cgcaatccat gagcgtggat gaggtgctta gccatgtccg
    40741 caaagcagtg ctgtttatcg aagggataac ggtgagtggc ggcgaagcca cgacccagtt
    40801 gccgtttgtg gtggcgctgt ttactgctat caaaaacgat ccgcaactgc gccatctcac
    40861 ctgtctggtg gacagtaacg gcatgttgag cgaaaccggc tgggaaaaat tgctcccggt
    40921 gtgcgacggc gcaatgctcg atctcaaagc gtgggggagc gaatgtcatc aacaactcac
    40981 cggacgcgat aatcagcaga ttaagtgcag catctatttg ctggcagagc gcggcaagct
    41041 ggcggaactg cgtttgctgg tgatccctgg ccaggtggat tatttgcaac acatcgaaga
    41101 actggcggcg tttatcaagg gactcggcga tgttccggta cgcctgaacg cgtttcatgc
    41161 ccacggcgtg tatggcgagg cgcaaagctg ggcgagcgcc acgccggaag acgttgagcc
    41221 gttggctgat gcgttaaaga tgcgcggggt gagccggttg atatttccgg cgctgtattt
    41281 gtgacatgcc ggatacggcc tacggttcgg cacagacttg taggcctgat aagacgcgtc
    41341 aagcgtcgca tcaggcattg tgcgccaact gccggatgcg gcgtgaacgc cttatccggc
    41401 ctacggttcg gcacaaacct gtaggcctga taagacgcga caagcgtcgc atcaggcatt
    41461 atgcgccgac tgccggatgc ggcgtgaacg ccttatccgg cctacggttc ggcacagact
    41521 tgtaggcatg ataagacgcg acaagcgtcg catcaggcat tgtgcgccaa ctgccggatg
    41581 cggcgtaaac gccttatccg gcctacggtt cggcacagat ttgtaggcat gataagacgc
    41641 gtcaagcgtc gcatcaggca ttgtgtgccg actgccggat gcggcgtgaa cgccttatcc
    41701 ggcctacggt tcggcacaaa cctgtaggca tgataagacg cgacaagcgt cgcatcaggc
    41761 attatgcgcc gactgccgga tgctgcctac gaagggtaaa ctacccacta acgctaaaca
    41821 acgcatacgt attattaagc aacacttcgg caatctcatc ttctggttcc gggcgcaact
    41881 cgcaaagcac gtcaaacacc cgcgcggcct gttccgggcg gttaggctgc ccctgaaaac
    41941 cgttgagcgg catatccggc gcgtcggttt ccagcaacaa cgatgccagc ggtaattttg
    42001 cgatgacatc gcgggtttta ctggcgcgtg gataggtgat agtgccgcct acgccaattt
    42061 tgtagcccag ctgtacaaac cgttcagcct gttgcaggct gccggaaaaa ccgtgaacca
    42121 cgccagtacg cggcaaatcg tggcgtttaa gatgcatcgc cagtttgtcg tgcgtgcgcc
    42181 gtgaatgcag gatcaccggc agatcgtagc gtttcgccag tttcagttgt tcgtcgagta
    42241 accactgctg cctctcaaat tgcggatcgt cgccaaagag atccaggccg atctccccca
    42301 ccgccaccac cttcgccgga tgcctttcca gcgcctgctg taactgctcc agcgatgcat
    42361 cgctatgttt ttctaacatc cccggatgca accctaacgc gccatacagc ggttgatatt
    42421 tttccgctaa cgcctgcacg cgggcaaaat tttccgcctc tgtcgccgga acaatgatct
    42481 tgcctacgcc cgcttgttcc gcgcgttgca ggctggcctc ttcatcacca ctaaacggcg
    42541 ggaaatcaaa atggcagtgg gtgtcgataa aacggcaaat caagccagat cctcattgtt
    42601 aaatgtcggg tcgttcgcct gcggagcgtc gatcacttcg gcaaccagcg tatcgttggc
    42661 gactgacgca ggcggaataa cgatcgactc cggcgtcacc accggcacca gatggcgggt
    42721 aagcggcgct ttttcagtca ataacttacc gaccgttgcc agaaaataac gaccgcataa
    42781 acgcccaagt ttatagtctt cacgcagcgc cggaatccga ctgccaagcg cgatactatg
    42841 caatggcttc ggcggataaa tttcgaatat ccgcagtttg cccggtggtt tctcaataaa
    42901 ttgctgaatg tcacgatagc tggtttcatg atgctgcacc agattgacca gcggctgcag
    42961 gctactgtca cccagccagc gttccatgcg tttgaaccac tgcggcgtgt agtacatttg
    43021 cgacggcacg gtgcggatga cgaccaacgt tttagcgccc tgccttgccg cctctttaac
    43081 cggaatcgca tcactgatcc cgccatccag gtagttaatg ccttccagcg acactccgct
    43141 acgataaaag ccaggtatcg cactggaggc gcgaatcaca tccagccagt tttgtttggt
    43201 tggtaaaaag taattcggcg cgtagtcatc ctgacgacag gcgcacatat aaaacgattt
    43261 gccgctgtca aacaaccgcg cggcggtatc catttgcaac ggcatctggc ttgcggtggc
    43321 ctccaccagc cagtcgagat cgataagatt tcctccacgg acaaagcgca atggatcgaa
    43381 aaattcgcgt tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg gctgattgca
    43441 gataaacgcc gagaggttct gcgccccggc agatgtgccg agataaagat cgaaaggatt
    43501 aaactgcgcg cgcataaact catccagcac gccagccgtg aaaattccac gctgtccgcc
    43561 gccttcgcac accagagcta ttcgtccagg ctggaacggc cttagcgaca acggcgcaat
    43621 attaccaagc gttacaggta ttcgctgccc cacctctgcc ttcctgtttt tgttgtgaaa
    43681 gaatgacaaa gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt ccgctggact
    43741 ggctttggat aaaattttct taatggtctg aaatctaggg tcgttttcgg cccatgaaca
    43801 aactcaccag gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg
    43861 taccggccag accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga
    43921 tgccccaacg aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc
    43981 gtgaaaatca cacaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt
    44041 cgggaggacg aattacgacg aattacttag ttttcagatc atttttaacg cttttcacac
    44101 catctaccgc tttggcgata ctttcagcac ggtcgctctg tgcctgggaa tcgacggtac
    44161 cggagagctg aaccacgccg tcggtggttt caactttcac atgacgggaa gggacgatat
    44221 cgtccgccag cagtttggct ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct
    44281 tcaccgagcc ttctttagcg tcgcgaacgt gcagtttgtc gctgacagag gtcacccctt
    44341 caacgccttt cgccactttc actgcctctt cggcctgagc ctggctttca acgaaaccgc
    44401 tcagggtcac gactttttga tcggttttga cagagatatc ggtgctctta atgttgtcat
    44461 gatccaccag cgccgccttc actttcgcgg tgatggcgct gtcatccatg aaattaccga
    44521 ctttattcat agagctatcg actttttgcc ctgcgctttc attggtagtc tgcgcgttgt
    44581 tttccgcgta ggcagagccg gtcgcgacgg cagaggtcaa cattacagcc agcagagttt
    44641 tcgaaatctt cagtcttgtc atagtcatcg atttattcct gtatgtttgc tcgtaatttg
    44701 agcctggcaa cacgaggttg cattgctgaa tagggagaga cttcaccctc tacagaagtc
    44761 aatggtcgcc atcacaaaag cgatgggtga tgaataacga ccattacagc ctctgaatca
    44821 gttattaata tcggtagaat gacaatcgac ggctttagat actgatatct acgcattgaa
    44881 cggtatttaa cgccgtcaga aatgtcatta ctttgttaaa tatagatcac aattttgaaa
    44941 ccgctcggga tatcagcgaa aacataagca aaagtgaatg ttttaagaac attccgtaag
    45001 cggctaataa ggaagggaaa ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa
    45061 ttaatgctcg cgggtctggt ggaactgaac gtccggataa cgttcctgcg ccagacgcag
    45121 gttgaccatg ctggtagcga tgtaagcgag gttatcgccg ccatcaagcg ccagttggct
    45181 ttcgttctta cgcttgaact cttcgaattt cttcgcgtcc gcacattcta cccagcgtgc
    45241 agtggcaacg ttgactgact catacactgc ttcaacgttg tattcgctct tcaggcgcgc
    45301 taccaccaca tcaaactgca gcacaccaac tgcaccaacg atcaggtcgt tgttggagat
    45361 cggacggaac acctgcactg cgccctcttc ggaaagctgt accagccctt tgagcagctg
    45421 tttttgcttc agtggatctt tcaggcggat acgacggaac agttctggtg cgaagttcgg
    45481 aataccggtg aacttcatca tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc
    45541 gtggttgtgc aggccgagga tatctcccgg atacgcttct tcaacgtgcg aacggtcgcc
    45601 cgccataaag gtcagcgcgt cggagatcac cacatctttc gcggtgcgca cctggcgcag
    45661 tttcatgcct ttttcatatt taccggatac cacgcgcata aacgccacgc ggtcacggtg
    45721 tttcgggtcc atgttggcct gaattttaaa tacgaagccg gtaaatttgt cttcgctcgc
    45781 ttctacggta cgggtatcag tctgacgcgg catcggcgca ggtgcccact ccaccagacc
    45841 atccagcata tgatcgacgc cgaagttacc cagcgcagta ccaaagaata ccggagtgat
    45901 ttcgcccgca aggaacaatt ctttgtcgaa ctcgttagac gcgcctttca ccagctccag
    45961 ttcatcacgc agctgctgcg ccagatcttc accaaccgca gcgtcgagat ccgggttatt
    46021 cagcccttta acaatacgga cttcctgaat ggtgtggcct ttaccgctct gataaagata
    46081 ggtttcatct ttataaaggt ggtaaacgcc tttaaacagc ttgccgcagc caatcggcca
    46141 ggtgatcggt gcgcagccga ttttcagctc gttctcaact tcatcgagca gttccatcgg
    46201 gtcgcggata tcacggtcaa gtttgttcat aaaggtgagg atcggcgtat cgcgcagacg
    46261 ggtaacttcc atcagcttgc gggtacgatc ttcaacacct tttgcggcgt cgataaccat
    46321 caggcagcag tccaccgccg tcagggtacg ataggtatct tccgagaagt cttcgtgccc
    46381 cggggtgtcg agcaggttaa ccaggcaatc gtgatacgga aactgcatca cagacgtagt
    46441 aatggagatc ccacgctgct tttccatctc catccagtcc gacttagcgt gctggttgga
    46501 accacggcct tttactgtac cggcggtctg aatggcctgt ccgaacagca gcaccttctc
    46561 ggtgatggta gtcttaccgg cgtctgggtg agaaataatg gcaaaagtgc ggcgcttcgc
    46621 cacctcttgc aaataaggag acaacgtcat aattctttct tcttgagtaa gcggcatcgc
    46681 gccgcgcttg ttgaaaacga aaaattgcgg ctattttacc catcaacggg ggggaggcaa
    46741 tcagtgttta cacaggagct gctccagttc gtgcaacgaa gaaacggtcc aggtaggcgc
    46801 gatgccttct ggttgctcgc gattgtgcgc attcagccag caggtcgcaa gcccggcgtt
    46861 gatgccaccg agaatatcgg actcggcagt gtcgccaacc atcagcacgc gtgaacggtc
    46921 aggattgccc gcctgttcca gcgcataatc gaaaattttc ttattcggtt tggcaacgcc
    46981 aacttcttcg gaaatcacca gcaaatcgaa ataatcacgc aggcccgtgc gttccagacg
    47041 cacttgctgc aaggcgctaa agccgttggt gatgatgcca attttagcgt tgccacgaat
    47101 ggcgttaagc agagaaaccg cgcccggcag cggcgtacag atttccgcca tcgcattaat
    47161 aaaggcttcg ttgagtttac ccggctcgac gttcagccgt tcggcccagc tctcaaaccg
    47221 cccgtgctga agctgtaatg aagtgatcgc gccgttttga taatccaccc acagtggctt
    47281 gttaacggcc tgatagtcct gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa
    47341 catccgctgc aggccggtga atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa
    47401 aatccagtcc cacttcatta ttccaccttg tcttacatac tgattggcaa cgccatgatg
    47461 atggcgtctt cgcgaccgtc cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta
    47521 aagcctaaac tttcgtacag tgcaatggcg gcagcgtttg aggcacggac ttccagccat
    47581 agcgtcgcca cgccgcgttt ttccagttcg tcgatcagat gttccagcag cgcccttccc
    47641 aatccctgac gctgatagtc aggatcgacc gcaatattga acaatgtagc ttcatccagc
    47701 accacttgcg taatcgcaaa cgccgccatt ttgccgtttt gcgttaactg aaagttgaga
    47761 taacgctcgc cctggttgct ggcaaaggtt ttctcactcc acggaaaggc gtgggcgcgt
    47821 tgttcaatgt ggtaagccgc tggtaaatca gtcgtttcga gggaagaaat cgtgttcata
    47881 tgtgcaaatt tgttgccata acgcggcgcg tgccgttggg tttgcccgta attcggtgag
    47941 cgccggtgat gccacctgag cgccttccag tgatagcggt tcgtcagtac ccaaccgcca
    48001 gctgttgcag cgactgcctt gtggcagcat cgagattttt tctggcgtca gttgcagcac
    48061 ttgatcgggg ctgacggtta atgcgcgcag aacatcgctc accagaggat cagtcagggc
    48121 gggaagatcg tttgccacca tcaccagacg gacgtgtgcc gggatcgcaa tggcaatctc
    48181 cccctgcaac gcgccagggc gacgcagcga ccactgggta atgcccagtt gctgtaactg
    48241 ccagtctcgt cgggatgtca tagcgaatcg ctcctgttgt caggggcgca aatatagcaa
    48301 attcgtcgat accgcgccaa catatggcta taatcgccgc cagtatcaat tgaggagcat
    48361 tccatgtctg catttacccc ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa
    48421 caaagccgta ttctgtttgc cggagactta caggatgacc tgcccgcgcg tttagatacc
    48481 gcggccagcc gtgctcatac ccagcaattc caccattggc aggtgttaag ccgccagatg
    48541 ggggataacg cccgttttag tctggtcgca acggcagatg acgtcgcaga ttgcgatacg
    48601 ctgatctact actggccgaa gaataaaccg gaagcccagt tccagttgat gaatttactt
    48661 tctctgctgc cggtgggcac ggatattttt gtcgttggcg agaaccgcag cggcgtgcgc
    48721 agcgccgaac agatgctggc agattatgcg ccgttgaata aagtcgacag cgcccgtcgc
    48781 tgcggcctct attttggtcg tctggaaaaa cagccggaat ttgatgcgga taaattctgg
    48841 ggcgaatata acgtggatgg cctgacggtc aaaacgctgc cgggcgtgtt tagccgcgac
    48901 ggtctggatg tcggtagcca gttactgctc tcgacgttaa ctccgcacac gaaaggtaaa
    48961 gtgctggatg tcggctgtgg cgcgggcgtg ctttctgttg cctttgcgcg ccattcgccg
    49021 aaaattcgtc tcactctgtg cgatgtctct gcgccagcgg tagaagccag ccgcgcaaca
    49081 cttgcggcca acggtgttga aggtgaagtc tttgccagta acgtcttttc cgaggtgaaa
    49141 ggtcgttttg atatgatcat ctccaacccg ccgttccacg atgggatgca aaccagcctc
    49201 gatgcggcgc aaacgctgat tcgcggcgcg gtgcgtcatc ttaatagcgg cggcgagctg
    49261 cgaattgtag cgaacgcctt cctgccttat ccggacgtgc tggatgagac atttggcttc
    49321 cacgaagtga tcgcgcaaac cgggcgcttc aaggtgtatc gcgccattat gacccgccag
    49381 gcgaagaaag gttaagtatc tgatatctgt cgccgcatac cgtgtcggat gcggcgtaca
    49441 cgccttctct gaccgacaaa cgaattatcc acaatccgct gatggacatt tttccagcaa
    49501 ttacacctcc ggcgataatt aactattgac gaaaagctga aaaccactag aatgcgcctc
    49561 cgtggtagca attcttttta agaattgatg gtatgcgaag gtggcggaat tggtagacgc
    49621 gctagcttca ggtgttagtg tccttacgga cgtgggggtt caagtccccc ccctcgcacc
//
LOCUS       DAZFZC010000025        48925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFZC010000025 DAZFZC010000000
VERSION     DAZFZC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.025"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            341..2029
                     /locus_tag="ACHKXP_001680"
     CDS             341..2029
                     /locus_tag="ACHKXP_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO7646710.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCASWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
     gene            2026..2940
                     /locus_tag="ACHKXP_001681"
     CDS             2026..2940
                     /locus_tag="ACHKXP_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7646711.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRVQQLAADARCGSVRMVGIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2972..3220
                     /locus_tag="ACHKXP_001682"
     CDS             2972..3220
                     /locus_tag="ACHKXP_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7646712.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3220..4392
                     /locus_tag="ACHKXP_001683"
     CDS             3220..4392
                     /locus_tag="ACHKXP_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7646713.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     DGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4427..5506)
                     /gene="lptG"
                     /locus_tag="ACHKXP_001684"
     CDS             complement(4427..5506)
                     /gene="lptG"
                     /locus_tag="ACHKXP_001684"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7646714.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSMGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(5503..6573)
                     /gene="lptF"
                     /locus_tag="ACHKXP_001685"
     CDS             complement(5503..6573)
                     /gene="lptF"
                     /locus_tag="ACHKXP_001685"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7646715.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLIDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6604..7164)
                     /locus_tag="ACHKXP_001686"
     CDS             complement(6604..7164)
                     /locus_tag="ACHKXP_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7646716.1"
                     /translation="MSSRLIIALIIMLLTPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLLEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(7176..8000)
                     /locus_tag="ACHKXP_001687"
     CDS             complement(7176..8000)
                     /locus_tag="ACHKXP_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646717.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHALKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            complement(8000..9349)
                     /locus_tag="ACHKXP_001688"
     CDS             complement(8000..9349)
                     /locus_tag="ACHKXP_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO7646718.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFLRHSLLYNAAGEKS"
     gene            complement(9395..10153)
                     /locus_tag="ACHKXP_001689"
     CDS             complement(9395..10153)
                     /locus_tag="ACHKXP_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646719.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(10185..10898)
                     /gene="yghS"
                     /locus_tag="ACHKXP_001690"
     CDS             complement(10185..10898)
                     /gene="yghS"
                     /locus_tag="ACHKXP_001690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7646720.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            11072..11764
                     /gene="yghT"
                     /locus_tag="ACHKXP_001691"
     CDS             11072..11764
                     /gene="yghT"
                     /locus_tag="ACHKXP_001691"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7646721.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11813..13312)
                     /gene="pitB"
                     /locus_tag="ACHKXP_001692"
                     /pseudo
     CDS             complement(11813..13312)
                     /gene="pitB"
                     /locus_tag="ACHKXP_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(13604..15463)
                     /gene="gss"
                     /locus_tag="ACHKXP_001693"
     CDS             complement(13604..15463)
                     /gene="gss"
                     /locus_tag="ACHKXP_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO7646722.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15668..16534
                     /gene="yghU"
                     /locus_tag="ACHKXP_001694"
     CDS             15668..16534
                     /gene="yghU"
                     /locus_tag="ACHKXP_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO7646723.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16563..16644)
                     /locus_tag="ACHKXP_001695"
                     /pseudo
     CDS             complement(16563..16644)
                     /locus_tag="ACHKXP_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16657..16905)
                     /gene="hybG"
                     /locus_tag="ACHKXP_001696"
     CDS             complement(16657..16905)
                     /gene="hybG"
                     /locus_tag="ACHKXP_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO7646724.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16918..17259)
                     /gene="hypA"
                     /locus_tag="ACHKXP_001697"
     CDS             complement(16918..17259)
                     /gene="hypA"
                     /locus_tag="ACHKXP_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7646725.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17252..17740)
                     /gene="hybE"
                     /locus_tag="ACHKXP_001698"
     CDS             complement(17252..17740)
                     /gene="hybE"
                     /locus_tag="ACHKXP_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO7646726.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17733..18227)
                     /gene="hybD"
                     /locus_tag="ACHKXP_001699"
     CDS             complement(17733..18227)
                     /gene="hybD"
                     /locus_tag="ACHKXP_001699"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO7646727.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18227..19930)
                     /gene="hybC"
                     /locus_tag="ACHKXP_001700"
     CDS             complement(18227..19930)
                     /gene="hybC"
                     /locus_tag="ACHKXP_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO7646728.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLERG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19927..21105)
                     /gene="hybB"
                     /locus_tag="ACHKXP_001701"
     CDS             complement(19927..21105)
                     /gene="hybB"
                     /locus_tag="ACHKXP_001701"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO7646729.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(21095..22081)
                     /gene="hybA"
                     /locus_tag="ACHKXP_001702"
     CDS             complement(21095..22081)
                     /gene="hybA"
                     /locus_tag="ACHKXP_001702"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO7646730.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(22084..23202)
                     /gene="hybO"
                     /locus_tag="ACHKXP_001703"
     CDS             complement(22084..23202)
                     /gene="hybO"
                     /locus_tag="ACHKXP_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO7646731.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23391..23678)
                     /gene="yghW"
                     /locus_tag="ACHKXP_001704"
     CDS             complement(23391..23678)
                     /gene="yghW"
                     /locus_tag="ACHKXP_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO7646732.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23797..24684)
                     /gene="yghX"
                     /locus_tag="ACHKXP_001705"
     CDS             complement(23797..24684)
                     /gene="yghX"
                     /locus_tag="ACHKXP_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO7646733.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDLEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLSWQRTLKWFDKYLS"
     gene            24841..25881
                     /gene="gpr"
                     /locus_tag="ACHKXP_001706"
     CDS             24841..25881
                     /gene="gpr"
                     /locus_tag="ACHKXP_001706"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO7646734.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25921..26415)
                     /gene="yqhA"
                     /locus_tag="ACHKXP_001707"
     CDS             complement(25921..26415)
                     /gene="yqhA"
                     /locus_tag="ACHKXP_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO7646735.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26606..27490
                     /gene="yghA"
                     /locus_tag="ACHKXP_001708"
     CDS             26606..27490
                     /gene="yghA"
                     /locus_tag="ACHKXP_001708"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7646736.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27649..28074)
                     /gene="exbD"
                     /locus_tag="ACHKXP_001709"
     CDS             complement(27649..28074)
                     /gene="exbD"
                     /locus_tag="ACHKXP_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO7646737.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28081..28815)
                     /gene="exbB"
                     /locus_tag="ACHKXP_001710"
     CDS             complement(28081..28815)
                     /gene="exbB"
                     /locus_tag="ACHKXP_001710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7646738.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29067..30254
                     /gene="metC"
                     /locus_tag="ACHKXP_001711"
     CDS             29067..30254
                     /gene="metC"
                     /locus_tag="ACHKXP_001711"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO7646739.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30394..31053
                     /gene="yghB"
                     /locus_tag="ACHKXP_001712"
     CDS             30394..31053
                     /gene="yghB"
                     /locus_tag="ACHKXP_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO7646740.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31093..31992)
                     /gene="yqhC"
                     /locus_tag="ACHKXP_001713"
     CDS             complement(31093..31992)
                     /gene="yqhC"
                     /locus_tag="ACHKXP_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO7646741.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32186..33349
                     /gene="yqhD"
                     /locus_tag="ACHKXP_001714"
     CDS             32186..33349
                     /gene="yqhD"
                     /locus_tag="ACHKXP_001714"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO7646742.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33454..34281
                     /gene="dkgA"
                     /locus_tag="ACHKXP_001715"
     CDS             33454..34281
                     /gene="dkgA"
                     /locus_tag="ACHKXP_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO7646743.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            34481..35407
                     /gene="yqhG"
                     /locus_tag="ACHKXP_001716"
     CDS             34481..35407
                     /gene="yqhG"
                     /locus_tag="ACHKXP_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO7646744.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35458..35715
                     /gene="yqhH"
                     /locus_tag="ACHKXP_001717"
     CDS             35458..35715
                     /gene="yqhH"
                     /locus_tag="ACHKXP_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO7646745.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35758..37977)
                     /gene="ygiQ"
                     /locus_tag="ACHKXP_001718"
     CDS             complement(35758..37977)
                     /gene="ygiQ"
                     /locus_tag="ACHKXP_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO7646746.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38088..39500)
                     /gene="ftsP"
                     /locus_tag="ACHKXP_001719"
     CDS             complement(38088..39500)
                     /gene="ftsP"
                     /locus_tag="ACHKXP_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO7646747.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMNDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39575..40312)
                     /gene="plsC"
                     /locus_tag="ACHKXP_001720"
                     /gene_synonym="parF"
     CDS             complement(39575..40312)
                     /gene="plsC"
                     /locus_tag="ACHKXP_001720"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO7646748.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40546..42804)
                     /gene="parC"
                     /locus_tag="ACHKXP_001721"
     CDS             complement(40546..42804)
                     /gene="parC"
                     /locus_tag="ACHKXP_001721"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO7646749.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(42942..44549)
                     /gene="ygiS"
                     /locus_tag="ACHKXP_001722"
     CDS             complement(42942..44549)
                     /gene="ygiS"
                     /locus_tag="ACHKXP_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7646750.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(44682..45077)
                     /gene="mqsA"
                     /locus_tag="ACHKXP_001723"
     CDS             complement(44682..45077)
                     /gene="mqsA"
                     /locus_tag="ACHKXP_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HFO7646751.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(45079..45375)
                     /gene="mqsR"
                     /locus_tag="ACHKXP_001724"
     CDS             complement(45079..45375)
                     /gene="mqsR"
                     /locus_tag="ACHKXP_001724"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HFO7646752.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(45580..46062)
                     /gene="ygiV"
                     /locus_tag="ACHKXP_001725"
     CDS             complement(45580..46062)
                     /gene="ygiV"
                     /locus_tag="ACHKXP_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO7646753.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(46115..46507)
                     /gene="ygiW"
                     /locus_tag="ACHKXP_001726"
     CDS             complement(46115..46507)
                     /gene="ygiW"
                     /locus_tag="ACHKXP_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO7646754.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            46659..47318
                     /gene="qseB"
                     /locus_tag="ACHKXP_001727"
     CDS             46659..47318
                     /gene="qseB"
                     /locus_tag="ACHKXP_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO7646755.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            47315..48664
                     /gene="qseC"
                     /locus_tag="ACHKXP_001728"
     CDS             47315..48664
                     /gene="qseC"
                     /locus_tag="ACHKXP_001728"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO7646756.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLNLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(48710..>48925)
                     /locus_tag="ACHKXP_001729"
     CDS             complement(48710..>48925)
                     /locus_tag="ACHKXP_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO7646757.1"
                     /translation="GNICSIPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFE
                     FWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT        12164 a        12897 c        12199 g        11665 t
ORIGIN      
        1 aataatcaac ccaatgaata caaacgacga ataaatagta tttattaatg attacgataa
       61 ggtcagagac tacacagaag cgccacaatg ccttttgtcg atttaaggcg accggacaag
      121 gcttcttctt aatatattca taaattaaat atcattcaca ataaaaacaa aaaaactcaa
      181 actcattatt taaatcaatg ttcattttca cactgatgaa aatactatct catttatctt
      241 ttatgcctat ataatccacc gcattattgt cattttattt tggatatcca attttttcgt
      301 ggtgtatatg tctaataaaa tctttacgca ttccctacct atgcgctatg ccgattttcc
      361 aacgcttgtt gatgctttgg actacgccgc tctgagtagc gccggaatga atttttatga
      421 cagacgttgc caacttgaag atcaactgga atatcagacg ttaaaaacgc gtgccgaagc
      481 tggtgcgaag cggttgttat cgctgaacct gaaaaaaggc gaacgcgtgg cgctgattgc
      541 cgaaacgagt agcgggtttg tagaggcttt ttttgcctgt cagtatgctg gcttagtcgc
      601 cgtcccgttg gcgattccaa tgggcgtcgg tcagcgggat tcctggagcg ccaaactcca
      661 gggtttactg gcaagttgcc agcccgcagc cattatcact ggtgatgagt ggttgccact
      721 ggtcaatgcc gcgacgcata acaaccccga attacatgtt ttaagccacg cctggtttaa
      781 ggcattaccg gaagccgatg ttgcgctcca gcgtccagtt ccgaacgata tcgcctacct
      841 ccagtacacc tccggcagca cccgttttcc ccgtggcgtc attatcaccc atcgcgaagt
      901 gatggctaat ctacgtgcta taagccacga cggcattaaa ttacgccctg gcgaccgctg
      961 cgcctcctgg ctgcctttct accatgatat gggactggtc ggctttctcc tgccccccgt
     1021 cgccacgcag ctttcagtag attatttgcg cactcaggat tttgccatgc gtcctctgca
     1081 atggcttaaa ttgatcagta aaaatcgcgg caccgtttcc gttgcgccgc cgtttggcta
     1141 tgaattgtgc cagcgccgcg tgaatgaaaa agatctcgct gaactggatc tttcctgctg
     1201 gcgcgtcgct ggtattggtg cagaacccat ctccgcagaa caactccatc aattcgctga
     1261 atgtttccgt caggttaact ttgacaataa aactttcatg ccgtgctacg gactggcaga
     1321 aaatgcgctg gctgtcagct tctctgatga agcctccggg gttgtggtta acgaagtgga
     1381 tcgcgacatc ctcgaatatc agggtaaagc cgtcgcgccg ggtgcagaga cacgcgccgt
     1441 atcgactttc gtcaactgcg gcaaagcgtt gccggaacat ggtattgaaa tccgcaatga
     1501 agcaggtatg ccggtcgcgg aacgtgtggt aggccatatt tgcatctccg gtcccagtct
     1561 gatgagcggt tactttggcg accaggtttc gcaagacgag attgccgcga cgggctggtt
     1621 agacaccggc gacctcggtt atctgctgga cggttatctg tatgtcaccg gacgcattaa
     1681 agatctgatt attattcgtg gccgtaatat ctggccgcag gatattgaat atatagcgga
     1741 acaggaaccg gaaattcatt ctggcgatgc gattgctttt gttaccgccc aggaaaaaat
     1801 cattttgcag atccagtgtc ggatcagcga cgaagaacgt cgcgggcagc ttatccacgc
     1861 gctggcggca cggatccaaa gcgaatttgg cgtgaccgcg gctatcgatc tgttgccgcc
     1921 ccacagtatt ccccgaacgt cctccggcaa gcctgcccgt gcggaagcga aaaaacgtta
     1981 tcagaaggct tatgctgcca gtcttaatgt gcaggaatcc ctggcatgaa tcaaactgtc
     2041 gcggtgacgg gcgctaccgg gtttatcggt aaatatatta tcgataacct gctcgcccgc
     2101 ggctttcacg ttcgcgcatt gacgcgtact gcccgcgctc acgtcaatga taatctcacc
     2161 tgggtgcgcg gttcgctgga agatactcat tcacttagcg agctggttgc cggagccagc
     2221 gtagtggtcc attgcgccgg gcaagtgcgc gggcacaaag aagagatttt cacccgctgt
     2281 aacgttgacg gcagcctgcg cctgatgcaa gcagcaaaag agagcggctt ttgccaacgt
     2341 tttctgttta tctcttcgct ggcggcgcgc catcccgagc tctcctggta tgcaaaatcc
     2401 aaatacgtcg ccgagcaacg actggcagca atggcggacg aaattacgct gggcgttttt
     2461 cgcccgacag ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt tgactggatg
     2521 ctgcgcggcc tgctgccacg acttggtgcg ccagatacac agctctcttt tctgcacgtc
     2581 accgatttcg cgcaagcagt gggtcagtgg ttaagcgccg aaactataca gacgcaaacc
     2641 tatgaattat gcgatggcgt cgccggcggc tatgactggc aacgcgtaca gcaacttgcc
     2701 gccgacgccc gttgcggttc cgtgcgaatg gttggcattc ctctgccggt actcaccggc
     2761 cttgcggata tcagtaccgc gttgagtcgc ctggcaggta aagaacctat gctgacccgc
     2821 tcgaaaattc gtgaattaac ccacgccgac tggtcggcaa gtaataaccg tatttctgaa
     2881 gatattaatt ggtttcccgg gattagcctg gaacacgcat tacgcaacgg gctattttga
     2941 ttgtcttgtt taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag taatggatta
     3001 catcctttcc tgtttacagg atttggtcga aaacggtgtg gaaattaagc ccgacagcga
     3061 tctcgtcaac gatcttggcc tggagtcgat caaagtgatg gatctcttaa tgatgctgga
     3121 agacagattc gatatttcca ttcctattaa tattctgctg gatgtcaaaa cccctgcgca
     3181 attaatggaa actctacttc catggctgga gaataaataa tggggctata cgataaatat
     3241 gcgcgccttg ctggcgagcg actgcaattt agtgacaacg ggttaacacc gtttggcacc
     3301 tgtattgatg aagtgtattc cgccaccgaa ggccggatcg gcaataaaaa agtgattctg
     3361 gccgggacca ataattatct cggtttaacc tttaatcatg atgccatcag cgaaggtcag
     3421 gccgcgttag cggcccaagg taccggcacc accggttctc gtatggcaaa cggcagttat
     3481 gcgccgcacc tggcgttaga gaaagagatt gccgagtttt tcaatcgccc taccgctatc
     3541 gttttttcaa ccggttatac cgctaattta ggcgtcatca gcgcacttgc cgaccataac
     3601 gccgtggtgt tactggatgc cgacagccac gccagtattt atgacgcctg ttctctcggt
     3661 ggcgcggaaa ttattcgttt ccgccataac gatgctaaag atctggaacg gcgcatggtt
     3721 cgcctcggtg aacgggcgaa agaggcgatt atcatcgttg agggtatcta cagcatgctc
     3781 ggtgacgttg cgccgttagc ggaaattgtt gatatcaaac gtcgtctcgg tggttatttg
     3841 attgtcgatg aagcgcattc gtttggcgtg ttaggcgcaa cggggcgcgg actggctgaa
     3901 gctgtcggcg ttgaagatga tgtggatatt atcgttggca ccttcagtaa aagtctggcc
     3961 tcgattggcg gttttgctgt cggttcggaa gcgatggaag tgctgcgcta cggcagccgc
     4021 ccgtatattt ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg ctcgtcgtta
     4081 agaactattg ccacacaacc agagctgcgg caaaagctga tggataatgc caaccatctg
     4141 tatgacggtc tgcaaaaact gggttatgag ttaagttcac atattagccc ggtagtgccg
     4201 gtaataatcg gttcgaaaga ggacggttta cgcatctggc gcgagcttat ttcgctgggc
     4261 gtttatgtga atcttatcct gccacctgcc gcgcctgcag gcatcacgct gctgcgctgt
     4321 agcgtaaacg ctgctcacag tcgtgagcaa atcgatgcca tcattcaggc atttgctact
     4381 ctgaagcagt aatgtgagcc cgccgtggtg ttgcgccacg gcgggtttaa tgttgtctgt
     4441 cataccaata caccaggctc aacgccacca gcacaaacgc tatcggtaaa ccgagcgcgg
     4501 taacaggtgc gctcaacgca aatagcaatc ccagattgac catgatttga tagctgatcc
     4561 aggtgagtaa gccaacgatg acacctacag cgagacggct acccatccct ggcgagcgcg
     4621 gggcgctaaa ggtaaacggc acagccagca aaatcatcgc cagggtcagg atgggttgcc
     4681 ccagtttttc ccacagtgca aggcggtatt cgctgctggg ttgcccggtt tcctgcaaat
     4741 aatggatgta atgattaagc tgacggacag aaaaagtatt gcccggcatt gataactctt
     4801 caagatccat actggtgaag gccgattgcc aggcaagatt atctaatgtt tccagcgttt
     4861 ctttgccatt cagccatttt ttatgattca caccatgcag gatccacgtt ttgtcgtctt
     4921 caatggtggc actgcgtgca taaatgtagg attcgagcga gagatcgtcg cgataatgaa
     4981 aaatctccac gcccaccggc tggccctgct cgttcagcga tttcaccgtc acaaattcat
     5041 taccacgcct ggcccacagc atattgccgg taatgtcatc gtcctccccc aacgcggtgg
     5101 cggtcgattt gatttgcaac gcctgttgct gtaatggcga cgctacccac tcatctatcg
     5161 cgcctaacga aacagtccac aatatccctg caaccagcgc caccagtgca atacggaaga
     5221 tagaaaaccc catgctgcga atggcggtaa gctcactgtt tttcgataac tgccccaggc
     5281 cgacaatccc tcccaataag gcgataaatg gcgaaagttc gaccagtgtg cgcggtaagg
     5341 tcattagcac caccagcacc gcctgagtcc agcgataacc gcccgggctg acatcatcca
     5401 gttcgttaat caggttgaag gtggtaaaaa gcggcagcaa tagccctgcg gcggcggcaa
     5461 aaccgagaaa gagatgacgg attaaatagc ggctgaaaac attcatcggg aaaatttctg
     5521 cagcaaagag aagtcgcgtg cgaccagcat cagcaggccc attagcatga gtccgggtac
     5581 taaccacaaa ccaggaatga ggggaatcgc gccgttagcc accagcgtac ggcagacgtc
     5641 accgccataa aaaatggcaa taaacagcaa cgtcagtggc aataacgttg aaaatcgccc
     5701 ttgccgcggt tttacccgac ttaatgaaat ggccagcagc gccatcaaca atgcggtcag
     5761 gccacggctt tgccgccatt gcagttcggc atgatcggct ggaaagacgg agcgcgccag
     5821 ctccgtgacc gatttcgctt tgcgtttgac gttagggctt tgatccagcg gattcagatg
     5881 taattgcaga ttacggtaga tctgttcgtt gtcatcacgc ccctgatgat cgataagata
     5941 ggcagtgccg ttgtgcaaca taacggtcgg tttctcgggt gatgggtcaa ccacatcaac
     6001 cgaacgggcg cggaaaatgc gggttcgatt ggcagtagaa gtataaatca gcgcgtcagt
     6061 caggcgatta ttatcctgat caaccgtctg cgaaaggatc attcgtccgt tatcgttagt
     6121 gttaaatttc tttgcccgca actggcgaac atccagctcc gactgcgact gctgctccag
     6181 ttgataaatc tgcgcataag cccacggacg accatacatc gacagaaggg tgacaaaaat
     6241 acttagcggt atcgccagat aaagaacggc tttgtacaag cgtcccggac tgccccccgc
     6301 cgcagagata gcggtaattt ccgagtcggt atacatctgc cctagcgtca cgccaactga
     6361 cacatacaga ccaacaggta acaacatctc cagtgcaatc agtactttgt agaaaacgat
     6421 atccagcaca acatcaagag ctaatgtccc atttgccgct tcggtcagat aacgctgtgc
     6481 ggagtagctg gcgaaaataa agatcagaaa accgacgata atcagcacca gacggcgcgt
     6541 accacgcatg atgtagtgtt caaccagctt catctcacta acgccctcaa tcctttgagt
     6601 tttttattca ttgatcagtt tctgcgccag cgcaataaca tgatctacct cttccggcgt
     6661 caggctgccg tcctttgccc actgaatttg cccggcttta ttcagcacca gaatggttga
     6721 actctgctcc agcaagcgcc aggcaacgcg tccctgtcca ttgccgtcaa taacgaattg
     6781 cgcccacgga tagcggcgtt tatttttttc gattttgcca acgacaaaat agccagtacc
     6841 aaagatggca tcatcagtgt tcacaatggt ggtgggctga aaacgatcct gcggaaagtt
     6901 tgccgcttca accgctttaa ttaacagtga gttcttcttc ttcgccgact tacgcccggc
     6961 gatatactgg ataatgcgga cttttcccgc taattgtgtg ctgttccact tttggtagtg
     7021 aatttcgtcg tcgctattca gaagcagctc gccgccttcc tggatataaa caggggtaac
     7081 cgttttgcca gtgacgaaat tgtgtgcctg cacacccggg gttaagagca tgataatcag
     7141 cgcaataatc aaacgagatg acatcattgc tccggttata gcggaaaacg cgaaatcagc
     7201 agctctaagc gctggcgaat atcgctatcc agcgccaccg gtgcatcata cgactggcgg
     7261 ttttccatat catccgccac catgtcgtcg tgtttatccc ccatcaggcc attaatggcg
     7321 gcgaggaaac gggaaatctg taacgcctct ttattttcag gatcaagcga tcttaaggtg
     7381 tcgtaataag aaacctgctg gctgatgatt tttcgcacat catgaaattc cctgccggtt
     7441 agtcgcggtt tagcaattat ctctttcagg gcatgcattt gcttttgccg ataggcaata
     7501 aaaccgctta cgctttctaa ctggctatgg cgtaatgaat agttcaccaa cgaccagacc
     7561 ggagagctta aataaatccg taacaggtta ccgtaatacg acacaatatt tttcttaccg
     7621 ttacgaaagc cgtcctgaaa acgccccaga aaaacggtcg ttttgccgaa taaaaatccg
     7681 gcctgatgct tctttaaata cagccgttgc gcaaaacgca ggtgcttgta gcgcgctcgc
     7741 atgtatttaa attgtttgcg ctcatcagca gtcagctcat cgcctttggc ttgtttgcga
     7801 actaaacgca gtaattcctg acgattaacg ccattgagcc agaattgcag gcataacgcg
     7861 taattatcat ggatgatttc ggtggtacaa cttagtgcaa tagtcggcgg caaactaacc
     7921 tgtgcatcaa cgacatcatc ttcttcaatc gcaacaaata gacgctcgct cgcctcaagc
     7981 gggaaatatt ttctcatcat caggatttct ctccggctgc gttataaagc aaggaatggc
     8041 gcagaaaaaa tgcctttaag gttggaatta acgagagcgc cacgtccaga cattgcccac
     8101 cgaagtaagc aaacgctgct cccagcacac caaacagatg acagaacatg aacagcaata
     8161 cgatgtagcc gattgaggcg atagtttgcg ccacgagaaa agcgcgctgt tttcccgcca
     8221 tcaatagcaa tgactcctgc gggaaaccca gcatcgaaat cacaattgcg cccagcatca
     8281 cctgaatcag gtcatacgct tcgagatact taacgccaaa caccagtgaa atgagcggct
     8341 tgccgacgat caacaccgcg agcgccacca gaataccgat tccgcccgcc agtaaaccgg
     8401 acttcacacc taacaaccac ggtctggtgg tgcgcggatc taaacgcatc acctctgggt
     8461 aaaaactttt acccagcaaa cccgctggcg ttccggcggc gtcgaagaat gtcatggcga
     8521 ttttaaataa cccggcggca gcgggtccta acacgatccc caccaacact gtgctgcacg
     8581 agttacgcgc cgaccagatg gagtgggcaa tgttggttga ccagacaaaa ctccacgcgc
     8641 ctttaatgtg tcgggcagac tcaaacagat tcaatttgaa ggcgttatgg atatttctgc
     8701 ggcgtaattc gcgcgcggca aaccaccagt acatggtgcc gccaaccaga ttcgacacgt
     8761 accaggcaat aacaaaaccc gcaaaaccaa agtcaaaata ccaggctgcg acgctccccg
     8821 ctgcgcgcag aaaaggtttc gtcgcctgct gtacagcaat caaatcaaag cgatctaccg
     8881 cacgcagaat gccggtcggc gtggaggaag ccattgaagg aatgagcgtg caatacagcg
     8941 ccgccagcca aaagctttgg tcatccagac ctaatgaatg agaaaggaat ggcagtaagg
     9001 caatgccgcc gacaatcgcc accgcgccgc tgacgatatc cagcgagaag gaaaatgaga
     9061 cgacattgcg aaattgctgc ggattattgt tggttaatgc tggtgttccg tactgaacca
     9121 ccagctgcca tgtctgaaac ttaataaaat cgctgatcga cttggcgtac gattgcacaa
     9181 tcaccagtac gccaaacatg gcgggcgtca tccctttacc ggcacacgag agcgccaaca
     9241 gacccagcaa ggcgctcacg acattactgg agcctaacca ggcgctattg cgaataatgg
     9301 tgcgaaacgc gccatctgca aaccaatgtt tgatgttaaa acccgccaat tcagcctgac
     9361 cttatcgttg atagtaaaaa gtatcccgcc agccttaagt taaacttcgg cggtcagaaa
     9421 cgatggcaac cagagaaacc gccttctgtg cctgttccag cacttcgctg tagggcgctc
     9481 tggaatcaat ctcaagaatt tttgtgccgt tatagccaat cttcgacatg acaccgattt
     9541 tgtcctgcag ctcggcatag tcatggtcag gcttgcggga gatggcagtc tcaatatcaa
     9601 tgcccaggcg aataattaat tccgggcgat attgcgccat ttgttggtat aaacgccgtt
     9661 cgcgctgcgc cagaaacatg ctgattttcc cggtcgcacg ttcgacgcca atccccggtc
     9721 catcataata aaagcccgaa atttcagcct gcgggaagcg atcgctgacc accagaacgc
     9781 cactttgcgc cagtcgctga accttgcgta gattcgccat tcttcgcagc gagaagcagt
     9841 acataatcac cgccgcccat agcgccggag atttggtttt catgctttgg gttttcgagg
     9901 atttggccgc cagtcgccgt tccagccaga cgccaaccaa cggcaatcgt ttgattttgt
     9961 cgccgtcttc gccggagagc agccccagat agcgccgctc ggtttgccag tgttgttgca
    10021 gcgatttcac caggtcggtg gtcagtgtgg atttaccggt gccatcacac ccgaccaccg
    10081 caatcagccc cggaatgtag ttgggctgcg gcgcggttgt actattaaca gtttgagttt
    10141 gtagtgcatc cattcgttaa atcatcccct atccattttg gtgatcagga aagcgcggcg
    10201 tgaattgcgc gtagtgacgc ttgcagaatt tcatcggcgg ggtgtcgccc atccagttca
    10261 aggatctttg cgccattgaa tgtcagttgc ggcgtaacgg cgattttttc ctgtaacgct
    10321 gccagttggt ggtcaggttt acgcgcaaac gcggtttgtt catcaatgcc aagacgaatc
    10381 aacaatacgg gcaaataaga tgccatccat tggtacagct tcagctcgcg ctgccttaac
    10441 atttttatcc aaccgttacc gcccgtggtt tttgccaatt gcgggccatc aaagcgaaac
    10501 cccggcactt caacctgcgg gtagcggtcg gtgatgagca gaaagccttg ctggctttta
    10561 cacaacattt tgcgaaactt gtacgcccgc cagcaggaaa gcagatagat aaccagtgca
    10621 gtaatattgc caggcggtgt tgagggcttt tcgtgcacat gtgccgcttt acttcgcaga
    10681 taacgcccaa aaggtgcgcc aataacaggg agctgtgaaa tccattcgcc aattcgcccg
    10741 gacgattgcc cgagataaat gtgttctgtt ggcattcttg ctgccagttc atttaccagg
    10801 cttgccgtga gggtcgattt acctgaacca tcacatccta caatggcaat aacacgcact
    10861 ggcgtggaat taattattga catattacgt tgattcacga aaaacccggc agtaaataat
    10921 gtattgaata ttacgtggtc cgtgctcaga atatccgttc aaccttattg tgacaatggt
    10981 taattgtgac attgacctga attacgcttt cataaaaaca tattaaccaa ataaatattt
    11041 ttaatggata tttaaattaa aggatatatt catgcagtca ataacacctc cattaattgc
    11101 cgttattggt agcgatggtt caggcaagtc aacggtgtgt gaacatctta ttaccgttgt
    11161 cgaaaaatat ggtgctgccg aaagagttca tttaggaaaa caggccggaa atgtcggtcg
    11221 tgcagtgaca aaattaccgt tgatgggaaa atccttacat aaaacaattg aacgaaatca
    11281 ggtgaaaaca gcaaaaaaat tgcctggacc agttccggcg ctggtaatta cagcgtttgt
    11341 cgcccgtcgc ttactgcgct ttcgtcatat gcttgcctgt cgtcgtcgcg ggttaattgt
    11401 tctaaccgac cgttatcctc aggaccaaat tcctggcgct tacgatggta cggtgttccc
    11461 acctaacgtt gaaggtggtc gttttgtctc atggctggca agccaggaac gtaaagcgtt
    11521 tcactggatg gcgagccata agcctgatct ggtcatcaaa ctcaatgttg accttgaagt
    11581 tgcctgtgca cgtaaacccg accataaacg ggaatcgctg gcgaggaaga ttgccataac
    11641 gccacagtta acctttggtg gtgcacaact ggttgatatc gatgccaatc agccactgga
    11701 acaggtgttg gttgatgcag aaaaagcgat tacggatttt atgaccgcgc gtggttatca
    11761 ctagtcaaaa atggaaatgc ccgatcgcca ggaccgggca ttttcaggaa ggttaaatca
    11821 actgcaatgc tatccagtac agcccaccag aaagaaaaat tgccgccggt aaagtaaata
    11881 cccacgccat caggatgctg gttaccgttt tacgctgtaa cccaccgccg tccaccacca
    11941 tcgtccctgc aactgcagac gagaggacgt gtgttgtgga gacgggcatc ccaatataac
    12001 tggcaagacc gatagacact gccgccgtca tttgtgccgc catgccttgc gcatacgtca
    12061 tgccgcgctt accaatcttc tcaccgatgg tcatcgctac acgacgccag ccaatcatgg
    12121 tgccaatgcc gagcgccagt gctaccgcca tgatgatcca caccggagcg tactcaatgg
    12181 tgcttaacat atcgctgcga agttttttca gcaggttctg gtcttcttta ctgacgcctg
    12241 gcagtttcgc tagcttcgcg gaggtatcag agatgcacag cataatgcgg cgcagctggc
    12301 tgcgctgact cacgcttaac ggctcgtaac tttccatatt gcctggcagc atcgttttaa
    12361 cgcgcgcaat agcatcaaag gtatttgccg gatgacagtg aaactctgtt acttgcgtgc
    12421 catcagtcga tgctgcaggc aatggaggtt acatcgcaat caacttctgc ggcagttcag
    12481 gatgctgttg caggtagtgt tcgaagttgg taacggcatc gcgggtacgg gtaatttcat
    12541 agccggacgc attcatattg acgacgaagc cagcaggggc aatccccacc agtaccagca
    12601 ttaccaggcc gatccctttt tgtccgtcgt tcgcgccgtg cgaaaacgcc acgcccgcag
    12661 cggaaacaat cagcgcaata cgcgtccaga atggcggttt acgtttgcct tttttctttt
    12721 tgcgatcttc cggaatgcgg tgaatacggt cacgcttttt cgtcccgctc cagtagcgtc
    12781 gcagcaggaa tatcaggcct cccgcaatga ccaggccgac gataggggaa acaatcagcg
    12841 aggagaaaat tttggtcact tcacgcaggt ttaacgcatc catcaccgat gagccggtta
    12901 acagcgcgtt ggttaaaccg atgccgataa tcgcaccaat caaggtgtgc gaactggagg
    12961 ccggtaaacc gaagaaccac gttcccaggt tccagataat cgccgccagc agcatggaaa
    13021 agaccatcgc caggccgtgg gttgacccca tattcagcaa caaatcggtt ggcaacatat
    13081 ggacaatggc ataggcaacg ctaagtccgc ccaataacac gccaaaaaag ttaaaaaatg
    13141 ccgccatcac cacagcaagt tgtggttgca tggcacgagt ataaataacg gctgccaccg
    13201 cattcgccgt gtcatgaaaa ccattgattg cttcgtagaa caacacaaat gccagagcaa
    13261 acaataacaa aagccctgtg tatatatcaa ggccaacaaa taaatttagc atatattaga
    13321 ttacgccatt ttgaatttac gaacggacgc attatcagtg actttaacgg catgggcaaa
    13381 gtgaaatatc attttttaac gataaaaaga gtgttatttc ttgtgtatca gacaataagc
    13441 attcattcaa aacctttaga aataatcaaa acacaggtat tccactggtt aatatattca
    13501 catatgaaat gaataaatat tggaatatat aaatattgaa tattttgatt aatccccgta
    13561 ctgattattc ttcataatca gtacggggtt gctacaacat gtattacttt ttcaccacaa
    13621 ttaacggttc aatatcactc tcttttttga tgaccagtga ttcatcaccg cgcaaacacg
    13681 tcccaccgta gttgccgcca acggtgaagg tacatacctg aatgtattta ccgtccactt
    13741 tcggcaaaca ccacagttgc tgatagatgt ttttctgctc ggcaaattta ccgctggttt
    13801 tgtccagcac ctcttcatga tggctgacga ggtcgatatt gctgccacag cgaccggcga
    13861 tcggtttcac tgcgtaacct gttttcacca gttcatcatt aacagtgaaa tcggtatcca
    13921 gcaggtaacg atggtgcggg aacagcgacc agaggatcgg cagaatcgct ttgttgccgg
    13981 ggatcaccgt ccacagcggc tcaaagacca gcacttccgg gcgcagcaat acgtcgataa
    14041 gacgcacttc gttttgcgga tgaccggtac ggattggcac cgcagcaaac tcacggtcgc
    14101 taacttcacg aatctgatca aacgcggttt cccacgccca ggttttccac acgcagttaa
    14161 ccagtcgccc ttccccatca atcagttgcc cggcagcatc ccagcccagt tcatccaatc
    14221 cacgcaagat acgcgtttca aagcccgcct ggtgcagcgc ctgctccata aactgcgcgt
    14281 gatagttttc ctcgatatct ttgtcctgca tgatatggac aaacggacgt gcacgactgt
    14341 gtttccaggc accagccaat tcgttaatca gcccttccgc cggattgaag ccgttgcctt
    14401 tatagccctg ctccgcccaa cgttcgagga tcaagcccgc ttcggtatga caggaggcgg
    14461 agtcggcgtt gtactcgtaa accttcaggc cacgctcatc catgcagaaa tccatacgac
    14521 cagtgatcat atggtgacgg cgacgctgcc aggagagacg caaacgtggc cagaggattt
    14581 tcgggatgtc gaacagcgcc agcaggttgt catctttcag caccttgtcg gttgcgtgaa
    14641 gatacatcag gtgaagctcg ttggtggctt taattagctc ctgctcggca ctctcggtaa
    14701 tggtgtagta atgataagga tcctgattga tcacctgacc gttggcctgc acataggcgt
    14761 tttgcagcgg atctttttca tccagccatt taccgtcaaa ctggcctttg ttttccaggc
    14821 gcgctccgct gattttcagc agctcgcctg caatttccgg ctgcggtaag ctgtattcag
    14881 tatcttccgt ctggatcatc cagcccagaa tggtggtgtc atcaaaagtg tctttcaggg
    14941 tatagcagcc gttttcgacc accatctcca gctcgcgcgt ccactgttgc ccttgcggca
    15001 acggggaatg aatcacgttc tgttccgcaa tacggacttt gttgccatgc aattgggtaa
    15061 tgatggcgac atggccagtg tctttaaatt caccgccttt atcccagata agaagcgcac
    15121 ccgcgaccgg cgcacgcggc gagccgttag gaaatgcctg caatggcagg atgttgtcat
    15181 taaccacttc tcgcaggaag cgcagcgaga aaatctccca cgccataccc acgtcagtaa
    15241 agaccacacc gtaattcaga aagagaaaac ggcgagcaaa ttcaacgcat tgccacttgt
    15301 ggcccatata ttcgtcgtcg atatagctac ggaatacggc gtcatcttcg tattcctgcg
    15361 gatcgagaga actgtaatct gaagagtaga ttgctacccc acctggggcg tagcccaata
    15421 atgtcccgaa cggggcatcc tggctggtcg ttcctttgct catcacttta cctttaacaa
    15481 tacaacctaa gcagttggcg taattgttgt gctctgatta cctgctcagc gacattaacc
    15541 ggacagaggt cagactaatc atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa
    15601 ttcacattct gtacagcaag tgacctgcta cactgcttca acactaccac tcagaaggca
    15661 actcactatg acagacaata cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc
    15721 tggcggcgcg ttcgccaata tcaatcgccc ggtttctggt ccgacgcatg aaaaaacgct
    15781 gcccgttggc aaacatccat tgcaacttta ttcgctggga acgccgaacg gtcagaaagt
    15841 aacgattatg cttgaggagc tgctggcgct gggcgttact ggtgcagaat acgacgcctg
    15901 gctgattcgt attggcgatg gtgatcaatt ctccagcggc tttgtcgaag tgaacccaaa
    15961 ctcgaagatc ccggcgctgc gcgatcatac gcataatccg ccgatccgcg tgtttgaatc
    16021 tggttcgatc ctgctttatc tggcggagaa atttggctac ttcctgccgc aggatttggc
    16081 aaagcgtact gaaacgatga actggctgtt ctggttacag ggcgcggcac cgttcctcgg
    16141 cggtggtttt ggtcactttt accattacgc accggtaaag attgagtacg ccatcaaccg
    16201 ctttaccatg gaagccaaac gtctgctcga cgtgctggat aagcaactgg cgcagcataa
    16261 gtttgttgcg ggcgatgagt acaccattgc ggatatggcg atttggccgt ggtttggcaa
    16321 cgtggtgtta ggtggtgtgt atgatgccgc tgagtttctt gatgcgggca gttataagca
    16381 tgtacaacgc tgggcgaaag aagtaggcga acgtccggcg gtgaaacgtg ggcgtattgt
    16441 taaccgcacc aacggaccgc tgaatgagca gttgcatgag cgccatgacg ccagtgattt
    16501 cgagacgaat acggaagata agcgtcaggg gtaagggttg gtgttcgtcg cagcaagcca
    16561 tccaggccgg ataaggcgtt cacgccgcat ccggcaatcg tgcacaatgc ctgatgcgac
    16621 gctgccgcgt cttatcaggc ctacgaaagc taatcatcac gcgctggtaa tgtcgtaatc
    16681 catttggcgc agtgcgtcta atgtggcttt ggcttcatct tcgtcgatga tgctcatggc
    16741 aaatccgacg tgtaccagca cccactggcc cagtagatcg gcagggttac cttcacaaat
    16801 cagggcgata ttcacatcgc gcttgatacc acatacttca acctgcgcaa gctggtgaat
    16861 atcttcaccg acagccagca cctggcctgg aacgccaata cacattatta actccggtta
    16921 ttcaacttca atacttttga cgatcagcga atcgccggta tcgacacgca accgctcgcc
    16981 atgacagatc ggacactgcg catcgtgctg atgaatctcc accacctggc tgcaatccca
    17041 gcaccaggcc tgggcgggtt tatagacgat atgtaaatcg cacccttgcg ccaccgttcc
    17101 gtggcagaca atttcaaaac taaaacggac ggcgctctcc tcaacgcagg agagcgcacc
    17161 aatttccagc cacacggcgg tgacgcgctt aacatcgtgc tgctccgcct gccgttggat
    17221 aatttcaacg gcgctctggc aaagagacaa ctcatgcatt ttcgccactc ctgcgaccaa
    17281 acagcagggc gcgacgccct gcgtgtggta catccggatt cgtgactggc agcgaaagga
    17341 tcattcgtgc gcagtcatcc gtcaggcgtt ggccctcttc aatcgacatg ctgtgcgaaa
    17401 gcggcgacat cagcgaacag gagagatatt gcgaaacacc gtccagttcg ccaacggtaa
    17461 aggtcatagt gccatacggc agttgcagac caattttttc actcactttg cgcagcggcc
    17521 agagttgatc cgggccgggg aagataactg cactcagcat ccacggggtg atcacacacc
    17581 ccgtccactg accttcgaac agcgtaaagt cagaaacata caccggcatt gaaggatgca
    17641 gaaaagagag atcgtgcatc gaacgccggg caatttcttc aaacgctgcc tgtacttgcg
    17701 ccttcgggga ggtctggaaa cctgctatct cttcagtcat gaatcgcctc ccgtgggatg
    17761 gcttccacgc cagattcacg cagcgcagcc agaacctgct caagcgcagg ttcaatcatc
    17821 gcttcgaccg tcggcgtcaa gccgatgtgc ggctccagtg attccggaat tacgccgacc
    17881 agggtcagtt ttttcggaaa ctcgccggtg aagcgcaggg ccgacaacac gtcggccagg
    17941 ccaagctgat gcggagagat tttgttggta aacaacgccg gaacttcttc atcccgcagg
    18001 atcatcatcg ttcccggcgc gctcttcttc gacacaatgg catccgcaat aatcagatga
    18061 tcgcgatttg ccatgtcgcc aagcagctcc attcccgccg tgccgccatc gaggatctca
    18121 acataatccg gcagaatgta tcgttgctct aacgcttcga caatccgcac gccgatggct
    18181 tcatcggtca gcaaaatatt gccgacccct aagactaaaa tacgcattac agaaccttca
    18241 ctgaaaccac ttcgttgccg tcggcatcca ctacgtgtac cgcacaggcc atgcacgggt
    18301 cgaaggagtg aatggtacgc accacttcca gcggtttatt cggatcggca accggtgtac
    18361 ccaccagcga ctgctcgtaa ggaccgacgt catcattgaa gttacgcggg ccagagttcc
    18421 aggttgatgg aacaactgcc tggtagttac tgataatacc gtctttgatc accatccagt
    18481 gagagagcat accgcgcggc gcttcgagga agccaacacc tttgaattca cccgttgccg
    18541 gaatgttcgg cttcacaaag gtggtgtgat cgcctttgcc gatattggtg atcagtgcac
    18601 tgtattggtt ttgcaggata tcctgcaatt cgcagcagtg aacggtacga ccaataatac
    18661 ggcccagcgt agagtgcagt tgcgccactt ccagcgtgtt accagtcagt ttctgataaa
    18721 tcgcaacgat ttcattcagt ttgttctggg tagattcgcg acctgccgcc agtttcacca
    18781 gcatattagc cagcggaccc acttctaccg ttttgccgta gaaagtcggt gatttcaccc
    18841 aggaatattt cccgtcgtca gaccaaccat cataagccgg aatggtggta ccttcccacg
    18901 gtgcctgcgg cgcttcgtct ttataccagg agtgcttcgc gctttcctga atgcctttga
    18961 tcaggtattc atcggaatga gaagtgatcg gacgatacga ggacagatcc gcattctcaa
    19021 tgtagccgcc agggaacagg aagctgccgt ttttactgtc ggtcgggaat tccggcacgc
    19081 tcaggtagtt caccgcacct ttaccacgct ccagccattc cgggtagaac gcagcgataa
    19141 ccgcggtatc gaccttgtaa acctgttcaa caaagtcgct cagcttgtcg atgaaagact
    19201 tgatgtacat caggcgctca aggttcagca cgcccagacc gtcgaggttg attgggttag
    19261 cgaccccgcc taccgccagg ttctgaatgt gcggcgtttt accgcccagc agcgccacga
    19321 cgcggttagc gtcacgctgg cactccagcg cctgcaggta gtgcgctacc gcaatcaggt
    19381 tcacttccgg cggcagtttc attgccgggt gaccccagta gccgttggcg aaaatcccca
    19441 actgaccgct ggcaaccaga tctttgatct tgttctgaac tttggtgaac tcttccgggc
    19501 tgttcaggtg ccaggtcgaa acgcctttca gcatttcaga ggctttggtt gggtcagctt
    19561 gcagtgcaga agtgatgtcc acccagtcca gcgccgaaag ctgatagaaa tgaacgatat
    19621 ggtcatgcgt ggtgtgcgca gccagaatga tgttacggat gtattgcgcg ttaaccggaa
    19681 cgtcgatatt cagcgcactt tctgccgcac gaacggaaga cagcgcgtga gtggtagtac
    19741 atacgccaca gatacgttgc acaatcatcc atgcatcgcg cggatcgcgg tttttcacga
    19801 tctcttccat gccgcgccac atggtaccgg aagcccatgc tttcgaaacg acgccatttt
    19861 cgatttcgca atcgatgcgt aaatgcccct caatacgggt taccggatca atagtaattc
    19921 tctggctcat gctttgctcg cctcatgacg attatgatcg ttttgtttta aaggaggaag
    19981 tatcggcagt agacgaatga gtacgatgta agcgcaaatc tcaatagcca caaaaccaat
    20041 agaaatcaac agttcttccc aggtcgggaa gtaggcgtaa ccgccgcccg ggttgaatgc
    20101 caccagcgaa taggtcagac gccaggttgc acaacctaac agtgcgctca gtgctgacag
    20161 gaacagcatg cgggaatcat tacgcagctt cgccacacgc agaacgacca gcgggaagag
    20221 catcagcagg acttcaatcc agaacatcac ggagtagaag tcaccggcaa acgctaacga
    20281 cagcttgtcg cgatagataa gctcgccaaa gcgcagcacg atgaaaatcg ccagcaacac
    20341 actgatggtg ttggtcagct taacaaacag actcttttca tccggaccgt tgccacgcag
    20401 acccgcctgc accagcgaac cttcaaagat gacaatcgag aagcccatga tgaacgccgt
    20461 cagcagcgag aacagcggca acatttcata gctctgccac aacggatgca ccttgtagcc
    20521 cgccgagatc atcagcgacc ccattgaaga ctggtgcatg gtcggcagca gcgcaccgag
    20581 cgcgatgatg aagaacatca ccttgtttag tcgctgtagc gacaccttcc accccagacg
    20641 ttcaaacagt gccggagcaa actccagtgc catcacgccg atatagatgg tcatacagac
    20701 cgccgtctcg aacagtaccg agttcacgtt gaagtgaccc ggaatgtaga agtacggcag
    20761 gttccagtag cgacccacgt cgatagtgat cgacaagcca cccagtgagt aaccaaacag
    20821 actcgccaac agcgccggac gcaccagcgg atggtattgc ccacggttaa agacgtatac
    20881 cgcccacgcc agcgcccagc cgccacaggc aaagccggtg ccaatcagca ggtcaaacgc
    20941 gatccacacg ccccacggga agccgccgtt caggtcagag acagagccca gaccgaacac
    21001 cagacgcttc acaatcagga gcatacagat gacgattaac ggtccaaaaa tcatgaccgg
    21061 tttactgatg attttgccgc ccagcggttg tggatcatga ctcatgatcg tctcctccgt
    21121 cgtgatggtc gtttttggtg ttgcgacgaa ccagcacggt taagcccgcc agcacagcca
    21181 gtggtagcat catgccttta tacagggtgt gttgaatatt ttcggaacgc gcaccggtag
    21241 aaagatcgtc cagtttcggc aggtcgagat tttcataagg cacacccgtc agtaccagaa
    21301 cctgagtacc gccgccctct ttctcgccgt acagatgcgg ataatatttc ggcaccgtat
    21361 gcaggtaagt gtcgccagat ttcagcgtct gacgcggata gtggtattcg ctgccaggct
    21421 tcagcgccag acgttttttc gcctccgcca tcagctcttc acgcgtaccg aaaatcaccg
    21481 cgcccgccgg gcacacttct acgcagccag gtagaccgcc tttatcgaga cgttccacac
    21541 ctttctggtt gcacagctcg cacttatgca gcgcaccaaa cgggttgttg tagtcgtact
    21601 tcggcacgtt gtacggacag gcgaccatgc agtaacggca gccggtgcac acgtctttgt
    21661 cgtaatggac aatgccggtt ttcggatctt ttttcagtgc agagaccggg cacacagaga
    21721 cacagttcgg atcgacgcag tgcatacact gtttcttaat gtacgcgtag ccgttctcct
    21781 cctggtcttt gttgacccct gtgccgctgg tccacacctg aatgatgtta ttggtatacg
    21841 gcgacagttt gtcgttgttc gaccaggtct gttccccttg cgggttacgt tcagggaaat
    21901 tgatatcctg acacttggtg acgcaagcct ggcagcctac gcacaaggtc gagtcgtaca
    21961 acatccccag cgatcccgga attggcgggc ggttttcagc agccgcatga ctgacagacg
    22021 gcagcgcgcc cgtcagcaat gccccgcagg aggctgcttt aataaaatta cgtctgttca
    22081 cggttattct ccccgtgagt cagcgttatc tttcttttgc tgacgaccca gttcacgcac
    22141 cgccatcacg ctgacaccgg caaccaaccc aaccacaccg ccgagcaaac caatagcgcc
    22201 tgcagagacg ttgccgccct ctttagcgtt aacatccggt ttctgtgaac gcggagtttg
    22261 attttcgacg ttggcaagct gatggatgcc tttatggaag ccgatacctt cttcgttaca
    22321 gccatagcac gggtgaccaa tcgccaccgg ccacacaccg ccaacatcgc agaattgcag
    22381 cgttgagcag ttgccgtaag tttctggccc tttacagccg aggtggtaca ggcaccagcc
    22441 ttcgcggtgg ccttcatcac cgaactcttt ggcaaaacga ccagcatcga agtgcgggcg
    22501 acgttcgcag tgttcgtgaa tcagacggcc ataggcgaag gtcggacggt ttttgtcatc
    22561 cagtttcggc ggtttgccgt aagtgatgat gtgcgcaacg gtcgcgagga agttgtgcgg
    22621 gttcggcggg cagcccggaa tattgataac ggttttgcct ggcagaactt cttgcaggct
    22681 gactgcgcca gttgggttaa ctccagctgc ggcaacaccg ccccacgcag agcaggaacc
    22741 gatagcgata atggctgctg cgccttccgc cgctttgcgg atgtgatcca caatcggctc
    22801 accggcaacc atgcaataaa taccgttatc ttttaatggg atggaaccat ccaccactaa
    22861 cacatactgc cctttgtact tctcgagagc gttatgtttg ttctcttcga cctgatgacc
    22921 gaaggcggcg gaaagcactt cgtgatactc cagagagata gtctccagta cgaggttttc
    22981 taccgttgga tgcgttgcac gaagcagaga ttccgtacaa ccggtgcact cctgcgcgcc
    23041 aatccagata actggcggac gctgcgggtt agtaaccgat tcggccatct ctgcagcggc
    23101 tttgctactt aaccccatgg tggcggctaa tgctgcacaa agcttcatga aatcacgacg
    23161 gttaatgccg tgagaatgga tgagggtgtt atctccagtc atttatagtt attccgttgc
    23221 gaagacctgg catatatttt gcctcaatcg caaaatcaat aatgcgatcg atgcgccatt
    23281 taccacacat ttattatggt tatcgatatc atgatactgc ggcggggcgc taaaacgaag
    23341 ggaagaagca ttagtgtaat taattaaagc aagataatac gtatgtttga tcaattttcg
    23401 aggcgataac cggccataaa aaagcgcaac gtagaacagg aattattctc acgaaagaaa
    23461 tccattacca tctctttatc cagtccatag cggcgcgtca gaatacccgc ttcccaggcg
    23521 ctaagctggc gatctccggt cttttcgtac atccggtgtg agtagcctaa tacaaaaccg
    23581 cgtttataat cggcacagta ttttgtcaca ttaaccgcac tgtcggcatg cgttgctttt
    23641 aatcccgcca ttaagccttt accaaaatgg ttattcatcg ttcacctcat tcgctatata
    23701 ttgttatata tagcgatatt ttaggcaaag aacgactttt gcgcacgcta ttttttcatg
    23761 cttgtttaag acgtaatccc gtaagagata aacctatcag gagagatatt tatcgaacca
    23821 tttcagtgtc ctttgccagg aaagatcggc ggcagatttg tcataacggg gcgtggaatc
    23881 attatggaat ccgtgattaa cccccggata gatatacgcc tcataaacct tattattggc
    23941 tttcaacgcc gcctcgtaag caggccagcc ctcgttgatt cgggtgtcca gttccgcgaa
    24001 gtggagtagt aaaggcgctt caatcttcgc cacatcggca gtgggtgcct gacgaccata
    24061 aaacggcacc gcgcaggcca gttccggata cgcgacagcc gccgcgttcg atacgccacc
    24121 gccatagcaa aatccggtaa tacccacttt gcctgtcgct tgcggatagc gttgcataaa
    24181 ctcaattgcg gcaaagaaat cattcatcag tttggttgga tcaacctgct gttgcagctc
    24241 acgaccttta tcatcatttc ccggataacc tccaacggaa cttaagccgt caggtgccag
    24301 ggcgatatac cccgccttcg ccactcgccg tgccacatct tcgatatacg gattcagtcc
    24361 acgattctca tgcaccacca ccacggctgg cgttttgccg ctcatttttg cgggtttcac
    24421 cagataaccc cgtacctcgc cgtgaccatt tggcgaagga tacgtgatgt actcagcaac
    24481 aatctccagg tcggtaaact ctacctgagt cgccagcgca taatttggct tgagcaaatc
    24541 aaacaacgct aatgccgtca tcccgcccac cgcatacttc gccgcaagat tgaggaactc
    24601 acgtttcgag attttcccgt gagcgtaata gtcgtagtaa tccaacaact cttgtgggaa
    24661 atctttggcg gttaaacgcg gcatcgttgc actcctcaac tggtgttttt ttaagcaaag
    24721 cataagcgcg tgtttttgcc caatttttcg tcactctgtg agccagacta cgggatacgc
    24781 gctggcgaat cgctaaacta gaaacattgt ttcgaaattg aacggtggaa aggagaggtc
    24841 atggtctggt tagcgaatcc cgaacgttac gggcagatgc aataccgcta ttgcggaaaa
    24901 agtggtttac gcctgcccgc gttatcgctc ggtttatggc acaatttcgg tcacgttaac
    24961 gcgctggaat cacagcgtgc gatcctgcgt aaagcgtttg atttgggcat tacgcacttt
    25021 gatttagcca acaattacgg gccgcctcca ggaagcgcag aagagaactt tggtcgcctg
    25081 ctgcgggagg attttgccgc ttatcgcgat gaactgatta tctctaccaa ggctggctac
    25141 gatatgtggc ccggccctta cggctctggc ggttcacgta aatacctgct cgccagcctc
    25201 gaccaaagcc tgaagcgtat ggggcttgag tatgtcgata tcttttactc tcatcgcgtc
    25261 gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt
    25321 aaggcgctgt atgtcgggat ctcctcttac tcgccagagc ggacgcaaaa aatggttgag
    25381 ttgctgcgcg agtggaaaat tccgctgtta attcatcaac cttcgtacaa tttactgaac
    25441 cgctgggtgg ataaaagcgg cctgctggat accctgcaaa ataacggcgt gggctgtatt
    25501 gcctttactc ctctggctca gggattgctg accggaaaat atctcaacgg cattccgcaa
    25561 gattcacgga tgcatcgtga agggaataaa gttcgtggtc tgacaccgaa aatgcttacc
    25621 gaagccaacc tcaacagcct gcgcttattg aatgaaatgg cacagcagcg tggacaatca
    25681 atggcgcaaa tggcgttaag ctggttgctg aaagatgatc gcgtgacgtc ggtattgatt
    25741 ggtgccagcc gcgcggagca actagaggag aacgtgcagg cgctgaataa tctgacattt
    25801 agcaccaagg agctggcgca gattgatcag catatcgccg atggcgagct gaatctgtgg
    25861 caggcgtctt ccgataaatg acctgttaat aacgggcgcg cgaaccgcgc ccgaatcaga
    25921 tcagtgatta tgacgagtca gtcggtcaag atagcccatc acaaatgcag aaagcacaaa
    25981 cgtcagatgg ataatgacgt accacatcag tttgttatcc gggacatttt tcgcatccat
    26041 aaagacgcgc agtaagtgaa tggaagaaat tgccacaatc gacgctgcta ctttgttttt
    26101 cagcgacgtt gcgtccattt tccccagcca gttcagcttc tctttgttct cggagatatc
    26161 cagctgtgag acgaaattct cataaccgga aaacatcacc atcaccagca aaccgccaac
    26221 cagcgtcata tccaccagcg acagcaacac gaggatcaaa tctgattccg ccatcgagaa
    26281 gatattcggc agtacgtgaa taatctcctg gaagaacttc agcgccaggg caactaacgc
    26341 cagcgaaagg ccaaagtaca cgggggcaag cagccagcga gaagcataca ttgcattttc
    26401 aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt
    26461 gagtaaacag cacgcaacag caaatcaaca tatacgttaa acctttgtcg cataattctg
    26521 aggtttaggg atcttcctgg ttcccttatc aacgcatcga gaacgtctac ccttaaagga
    26581 cgttgaacct gaagggagaa aaacgatgtc tcatttaaaa gacccgacca cgcagtatta
    26641 cactggtgaa tatcccaaac agaaacaacc gacgccaggc atccaggcga agatgacacc
    26701 ggtaccggat tgcggcgaga aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc
    26761 actggtgaca gggggcgatt ccggaatagg tcgcgctgcc gccatcgctt acgcgcgtga
    26821 aggggctgac gtggcgatca gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa
    26881 aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg ccaggcgatt taagcgatga
    26941 gaaatttgcc cgttcgctgg ttcacgaagc gcacaaggcg ttaggcgggc tggatattat
    27001 ggcgctggtc gccgggaaac aggttgccat tccggatatt gcagacctca ccagcgaaca
    27061 gtttcaaaag acctttgcca ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat
    27121 ccccctgcta ccgaaaggtg caagtatcat caccacttcg tcaatccagg cataccagcc
    27181 aagtccgcat ttactggact atgcggctac gaaggcggcg attctgaact acagccgtgg
    27241 cttggcaaaa caggtcgcgg agaaaggtat tcgggtgaat attgtcgcgc caggcccgat
    27301 ctggacagca ctgcaaattt ccggcggaca aacgcaggat aagatcccgc agtttggtca
    27361 gcaaacgccg atgaaacgtg cggggcaacc ggcggaactg gcccctgtat atgtttatct
    27421 ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac ggcgtgtgcg gcggcgagca
    27481 tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg catcattgtg aattcactct
    27541 tacctgatgt ggcgaaaacg ccttatccgc ctacaaacgc atgaagattc aacatgttgc
    27601 aatagatttt gtaggcctga tgagcgtagc gcatcaggca attctacttt acttcgcttt
    27661 ggcggtttct tcgccgacca gacctatctt caggtaaccc gcctgatgca gcgtatccat
    27721 taccttcatc aacgtctcgt aatcgacggt tttgtccgct cggaagaaga tggtggtgtc
    27781 tttcttgcct tcggttaacg cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc
    27841 gttaccgata aacatcgagt tgtctgcctt caccgacaga taaaccggtt tttccgggcg
    27901 cggctgcggc gtgctggtag aagcaggcaa gttcaccttc acatctaccg tcgctaacgg
    27961 tgccgccacc ataaagataa tcagcagaac caacatcacg tcgataaacg gcgtcacgtt
    28021 gatatcatgc atttcgccgt tatcgtcgag gttttcgtta agatgcattg ccatcggata
    28081 ttatcctgcg cgtaattttt gtgcgacacg aaccggatgc gcagcggcgc tggcttccag
    28141 atccaggtca cggctttgca gcaacaatac ctgcgctgca acatcaccca gcatcgcttt
    28201 aaagccgcca atctggcgtg caaatacgtt atagataacg accgcaggaa tcgctgccac
    28261 gaggccgatt gccgttgcta acagagcttc tgcgataccc ggcgcaacga ctgccaggtt
    28321 agtggtctgc gtttgcgcga taccaataaa gctgttcatg atgccccaga ccgtaccaaa
    28381 cagaccaacg aacggagaaa tcgcgccgat ggttgccaga tagccgttac cgcggcccat
    28441 ttgacgaccc actgcggcga cccgacgctc cagacggaag ctggtacgtt ctttaatacc
    28501 ttcgttatcg tcgctgcctt ctgacagttc cagctcattc tgcgcttcat tgagcaaatg
    28561 caggcttaag cttttgctac caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg
    28621 cgcttcagcc agcagttgct gctcgcgctt aaggcgacgc ttctgattga agaactctac
    28681 gctcttactg aagaagattg cccaggtgac tacggaggcc aaaataagcc caatcatcac
    28741 gcacttaacg acaatatcgg cgtgctgata cataccccag acggaaaggt ccgtctgcat
    28801 taaattatta cccactgtgt atctccagga cgcaagtcac aaaatctgcg cataataata
    28861 tcaaaacgac gtcgaattga tagtcgttct cattactatt tgcatactgc cgtacctttg
    28921 ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt
    28981 tctgctttat gccaattctt caggatgcgc ccgcgaatat tcatgctagt ttagacatcc
    29041 agacgtataa aaacaggaat cccgacatgg cggacaaaaa gcttgatact caactggtga
    29101 atgcaggacg cagcaaaaaa tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt
    29161 cttcgctggt atttgagagt atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg
    29221 gcgagttgtt ctatggacgg cgcggaacgt taacccattt ttccttacaa caagcgatgt
    29281 gtgaactgga aggtggcgca ggctgcgcgc tatttccctg cggggcggca gcggttgcta
    29341 attcgattct tgcttttgtc gaacagggcg atcatgtgtt gatgaccaac accgcctatg
    29401 aaccgagtca ggatttctgt agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt
    29461 ttgatccgct gattggtgcc gatatcgtta agcatctgca gccaaacact aaaatcgtgt
    29521 ttctggaatc gccaggctcc atcaccatgg aagtccacga cgttccggcg attgttgccg
    29581 ccgtacgcag tgtggtgccg gatgccatca ttatgatcga caacacctgg gcagccggtg
    29641 tgctgtttaa ggcgctggat tttggcatcg atgtttctat tcaagccgcc accaaatatc
    29701 tggttgggca ttcagatgcg atgattggca ctgccgtgtg caatgcccgt tgctgggagc
    29761 agctacggga aaacgcctat ctgatgggcc agatggtcga tgccgatacc gcctatataa
    29821 ccagccgtgg cctgcgcaca ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga
    29881 aagtggctga atggctggca gaacatccgc aagttgcgcg agttaaccac cctgctctgc
    29941 ctggcagtaa aggccacgaa ttttggaaac gagactttac aggtagcagc gggctatttt
    30001 cctttgtgct taagaaaaaa ctcagtaatg aagagctggc gaactatctg gataacttca
    30061 gtttattcag catggcctac tcgtggggcg ggtatgaatc gttgatcctg gcgaatcaac
    30121 cagaacatat cgccgccatt cgcccacaag gcgagatcga ttttagcggg accttgattc
    30181 gcctgcatat tggtctggaa gatgtcgacg atctgattgc cgatctggac gccggttttg
    30241 cgcgaattgt ataacattgc cacttttgga caattttgca gacatttaat tgtgaaaagt
    30301 cttaaattgt tgcgtccggg atcaaggcgt cccggacgat tcaggagtac aataggcaga
    30361 taaaggctta aacgctgttc cacaggaaag tccatggctg ttattcaaga tatcatcgct
    30421 gcgctctggc aacacgactt tgccgcgctg gcggatcctc atattgttag cgttgtttac
    30481 tttgtcatgt ttgccacgct gtttttagaa aacggcctgc tgcccgcctc atttttgcca
    30541 ggcgacagct tgttgatact ggcaggcgca ttgattgccc agggggttat ggattttctg
    30601 cctacgattg cgattctgac cgccgcagca agtctgggct gctggctaag ttatattcag
    30661 gggcgctggt tagggaatac caaaacggtg aaaggctggc tggcacagct tcctgctaaa
    30721 tatcaccagc gcgccacctg catgtttgac cgccacggtc tgctggcgct gctggctgga
    30781 cgttttcttg catttgtccg tacgctgctg ccaaccatgg cgggaatttc cggtctgcca
    30841 aaccgccgct tccagttttt caactggtta agtggattgc tgtgggtcag cgtggtaacc
    30901 agttttggct atgccttaag tatgattccg ttcgttaaac gccatgaaga tcaggtaatg
    30961 acgttcctga tgatcctgcc aattgccttg ttaaccgctg gcttgttagg cacgctgttt
    31021 gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt ccccgttccc ggttgctgta
    31081 ccgggaacgt atttaattcc cctgcatcgc ccgcattctt gccgcatctt cccccggcgt
    31141 cacaccgaag taacgtttaa actcacggct aaattgcgat gcgctttcat agccgacgcg
    31201 catcgctgct gcgctggcct tcatgccgtc atggatgatc atcatccgcg ccttatgcag
    31261 acggtaattc ttcaaatact gcaacggcga ggtgctggtg acagacttaa aattatggtg
    31321 gaacgccgat acgctcatgt tggcttctgc cgccagttgc tcgacgctca ggttttcggt
    31381 gtatttattc tcaatccgtt tcagcacgcg gctaatcaga ctgaagtgag tctggcgact
    31441 gaccagcgcc agtaacgcgc cgccgcaagg tccggtcagc acgtagtaaa gaatttcgcg
    31501 gatgatctgt ttgccgagaa tacgcgcatc cagcggtcgc tccatcacgt cgagtaaccg
    31561 ctccgccgca cataaaatct cttctgataa cgtggcggag ttaatcccgc tggctgccat
    31621 cgacggctgg aaatgctcat cttcgccaat gtccatcaac agttcctgta actgcaaaat
    31681 atcgacattg agacgcaacc ctgccagcgg cacctctgac gtggcatagg tttcgcactc
    31741 aaacggcaac ggcaccgtca gcagcaggta ttcattggca tcataacgaa acacgcgttc
    31801 attgatataa ccgattttat gtccggaaaa gagaattatt atgccaggct cgtacatcac
    31861 cggtgtacgt gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga
    31921 caaactattt tctttatttt tcagtttatt aactttatcc gccagcaagc ggcaaatctc
    31981 ttcacgtttc atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt
    32041 ttgtagcatt tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg
    32101 agagccaggg cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat
    32161 attggcccag caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat
    32221 tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt
    32281 attgattacc tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga
    32341 tgccctgaaa ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga
    32401 aacgctgatg aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt
    32461 tggcggcggt tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga
    32521 aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc
    32581 gatgggctgt gtgctgacgc tgccagcaac cggttcagaa tccaacgccg gtgcggtgat
    32641 ctcccgtaaa accacaggcg ataagcaggc gttccattct gcccatgttc agccggtatt
    32701 tgccgtgctc gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt
    32761 agtggacgcc tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat
    32821 tcaggaccgt ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct
    32881 gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct
    32941 gaacggtttg attggcgctg gcgtaccgca ggactgggca acgcatatgc tgggccacga
    33001 actgactgcg atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact
    33061 gtggaatgaa aaacgcgata ccaagcgcgc taagctgctg caatatgctg aacgcgtctg
    33121 gaacatcact gaaggttccg atgatgagcg tattgacgcc gcgattgccg caacccgcaa
    33181 tttctttgag caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc
    33241 catcccggct ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca
    33301 tgacattacg ttggatgtca gccgccgtat atacgaagcc gcccgctaag ctttttacgc
    33361 ctcaaacttt cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc
    33421 ctgctccggt gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta
    33481 caggatggca atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa
    33541 gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg
    33601 gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa
    33661 gaactgttca tcaccactaa gctgtggaac gacgaccaca agcgcccccg cgaagccctg
    33721 ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc
    33781 gttcccgcta tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag
    33841 ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt
    33901 gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa
    33961 cgccagctac acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta
    34021 gcgcaaggag ggaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac
    34081 ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc
    34141 ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc
    34201 gacaaagacg aactcggcga aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat
    34261 cctgaccagt tcggcggcta acatgcaaat tctcccggtg gcggtaatgt tccgctaccg
    34321 gacttttcag aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca
    34381 ggaatgccaa atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca
    34441 tctatatccg ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt
    34501 tttagcagcc ctggcaagtt ttaccgtaca cgcacagccc ccctcacaga ccgtagaaca
    34561 aacagtccgg catatttatc agaactataa atcagatgcc actgcccctt attttggtga
    34621 aaccggagag cgggcgataa cttctgcgcg tattcaacag gcgcttaccc tgaacgacaa
    34681 tcttacgctg ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga
    34741 ttttggcgat ctggtgctag aaagcgttgc gataacccaa actgacgccg atcatgccga
    34801 tgccgttgtg cgctttcgta tctttaaaga tgataaagaa aagaccacgc agacactgaa
    34861 aatggtggcg gaaaatggtc gttgggtcat tgacgatatt gtcagcaatc atggcagcgt
    34921 cttacaagca gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcaaaa
    34981 agaacagccg gaagcctttg ttgccgaact ctttgaacat attgctgatt atagctggcc
    35041 gtggacgtgg gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac
    35101 cttcaagacg gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga
    35161 caatccgatc tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct
    35221 ggagaaaacc gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa
    35281 cgaagagcaa gaactggttt tacagcggcg cgaaggcaag tgggaaatcg ctgattttat
    35341 ccgcccgaac agcggcagcc tacttaagca gattgaggca aaaactgccg ccagattaaa
    35401 gcaatgagct gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg
    35461 aaaacgattt tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc
    35521 aatgagcaaa aggtcaatca gctggcgagc aatgtgcaaa cattaaatgc caaaatcgcc
    35581 cggcttgagc aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct
    35641 aacagagcca atacgcgtct tgatgctcag gactattttg attgcctgcg ctgcttgcgt
    35701 atgtacgcag aatgataaaa aaatccccgg tagcgtgtca gttgccgggg atatttttta
    35761 acgtccaacc gccgctttag gccgtttctt cgcaccagca ttcaccggac gagattgcgt
    35821 agacgacgct ttttttgccg tagcaggcgt ctgacgctgg gtcgccatcg gcgtatgttt
    35881 cgtcaacgcc ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt
    35941 tggcgcaggc actaagcaat cgcgacggct accaatcaga tgctttttac ccatcgcttc
    36001 cagcgcctgg cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg
    36061 caaacgacgc tgcttgtcgc ccttcggtac gaagacgtct tcactcttat aaccaatctt
    36121 cgccagcggg tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta
    36181 gaagttctgt acctggtcga gacggaaacg atgctttttc agccacagcg ccagattcac
    36241 catatcttca tcacgcgtac cggggtgcgc ggagatgaaa taggggatca gatactgctc
    36301 tttacctgcc tgttttgagt aagtatcgaa cagctcttta aagcggtcat agctgcccat
    36361 gcccggcttc atcatcttcg ataacggccc ttcttcggta tgttccgggg caatcttcag
    36421 ataaccgccg acgtgatggg tcgccagttc tttgatatag cgcggatctt ctacggctat
    36481 gtcataacgc acaccagagg caatcaggat ctttttaatg cctttcagat cacgcgcacg
    36541 gcgatagagg ttgatcgttg gttcgtggtt cgtgtccatg tgcggacaaa tatccggata
    36601 aacgcacgac aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata
    36661 catgttggca gttggcccac caagatcgga aatcacgccc gtaaaacctg gaacggtgtc
    36721 gcggatcgct tcgatctcat taatgatcga atcttcggaa cggctctgaa taatgcgccc
    36781 ttcgtgctcg gtgatagaac agaaagagca gccgccaaag cagccacgca taatgttgac
    36841 cgaaaaacgg atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac
    36901 gcgcttgtat ggcagcgcaa aaacgctgtc catctcttcg gtagaaagcg gaatagcagg
    36961 cgggttgatc cacacatagc ggtcgccgtg tttttgcatc aatgcgcggg cacagcctgg
    37021 gttggtttcg tggtgcagaa tacgcgaagc atgggcgtac agcactttat cgcccttcac
    37081 tttctcgaaa gaaggcagca acacgtaggt tttttcccac ggtttcgggc gcggtggctg
    37141 cacggttacg gctttggctt cctgcttttt cggtgccacc ggtttgttat ccgcgcacgg
    37201 caaatcttca ccatacggat gcgggattgg gtcgattttt ccaggggtat caagacgggt
    37261 ggaatccacg ccgctccagc caggcagcgc ctctttcacg ataatcgcgg tattacgcac
    37321 atcgcggatt tcactaattg gctcgcccat cgccagacga tgcgccactt ccaccagcgg
    37381 acgctcaccg ttaccaaaca tcagcatgtc ggctttcgaa tccaccagca cggaacggcg
    37441 cacggtatcg gaccagtaat cataatgcgc ggtacggcgc agactagcct caataccgcc
    37501 gaggatcacc ggtacatctt tccacgcctc tttacaacgc tgggtataaa ccagtgtggc
    37561 gcgatccggg cgcttacccg cgacgttatc cggcgtgtag gcatcgtcat gacgtaaacg
    37621 gcgatcggcg gtataacggt tgatcatcga atccatgttg ccagcagtaa caccgaaaaa
    37681 cagattcggt ttacccagac gcataaagtc gtctttgctg ctccagtccg gctgggcgat
    37741 gatcccgacg cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct
    37801 tgggtgatcg acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag
    37861 ttgatccatc tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca
    37921 gtactgcggc caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt
    37981 gcttccagaa atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag
    38041 aaatcgaaag gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga
    38101 ttgaccaaca gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag
    38161 tagaacggga agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg
    38221 tcaacccaaa cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg
    38281 ttacggatct ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc
    38341 gtccagcgtt cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc
    38401 tgtccattaa tacccgggtc atcacccaga ctgatatcgc gactgcgaat tggcgaacca
    38461 gccatgattt cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg
    38521 ccggttgggc gcagcgttag caccagggta gaaaccagaa tactggatgg ctcaaagaag
    38581 ccacgaatac gatcaacaat gctcgccgct tcgccacagg tgatcgacac ttcatcgccg
    38641 ttgctcatat ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc
    38701 gatacaggag caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg
    38761 ttcatctgta gttgatagcg acgagagttc gacgcgttca gcagtcgcaa gcgcacccag
    38821 ccacgcgaga cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca
    38881 aagccgccgc ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc
    38941 tggataatga ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg
    39001 acttcatctt ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg
    39061 ttgggagtat tggcgtgata ccacagagta gctgcgttct ggcgaatggg cagtacgggt
    39121 gcccagtcag cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc
    39181 tgtagcccgg ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta
    39241 acatcgtcgc ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag
    39301 accgacgcgc gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc
    39361 ggttgcccac ggcgagattc aagtagcggc ggaacgggta gcggttgctg ttgcccggct
    39421 gcgctggcct tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac
    39481 tgacgccgac tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag
    39541 gaaagtgttc cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt
    39601 ctgcgacttc tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag
    39661 ccagctcacg aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga
    39721 caatcaccag accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc
    39781 acacgggaat aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg
    39841 gtagcaggcc gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc
    39901 gttttttgaa gtgattcact acttccgcaa tggtgccgtg agctttagtg cgattgtttc
    39961 tgtcgatcaa taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca
    40021 agctcttttt acctaccgtc accgtcggcg gttgcacgat gttcgatgct gtcaccatgt
    40081 catagttgtt ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag
    40141 gtttgcggca ctcaactttc aggccgaaca gcggcgcaag acggccaaac atatgcccaa
    40201 aggtggccac atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac
    40261 agactaagat gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct
    40321 ctgaaggtct gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa
    40381 agccgctctc tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag
    40441 ggaattttta tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc
    40501 cggcgttcct tgcaagcggg aggaaacagc gccctccccg gcatattact cttcgctatc
    40561 accgctgctg gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg
    40621 catcaacgta ccgcggcgtc cacgttcgcc agtgactttc tgtaactctt ccgggcgcag
    40681 tttaattttg cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacaa
    40741 ttgcgccaga ccatcttctc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt
    40801 gcctttgccc ttcgacagct gcggcagatc acttaccggg aacatcaaca tacggcctgc
    40861 ctgagtgatt gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg
    40921 ggcattttcc ggtaaggtga tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt
    40981 aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct
    41041 ttccatcagc atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagcgg
    41101 ctcgccctga ccacgcgctg acggcagcgt aatcgggtca atggcatagc tacgaccggt
    41161 ggaatcaaca aacactaccg gttggttgct cttacctttc accgccgctt tgaagctatc
    41221 acccgcttta taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca
    41281 gcccatctgc gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat
    41341 cgctttcgct tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc
    41401 gtctgcctgc agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc
    41461 ctgcaactgg tcgcgctctt tttccagttc actctgctca ccgcgaatct tcatctcttc
    41521 cagtttggca agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag
    41581 gccaaaccgc gacatcagcg ccggtttcgg ttcatcttca ttacgaatga tctcaatcac
    41641 ttcgtcgata ttgagaaacg ccaccagcaa accttcgagg atatgcaggc gcttgaggac
    41701 tttctccaga cgatagttca gtcggcggcg cacggtatcg cggcggaaca ccagccattc
    41761 ggagaggatt tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattaag
    41821 gttaatacga tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc
    41881 catatccacg cggttggaac gcggcacaat caccaggcgg gtcgggttct cgtggtcaga
    41941 ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg
    42001 ctccagtacg cgcgcacctg aaacctgatg cggcaatgcg ctgataacca ccgcgccatc
    42061 ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg
    42121 gatctcggcg cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat
    42181 atccagcagc tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc
    42241 cacttcacgc aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt
    42301 gccgttaagc aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc
    42361 gtcgaagttt ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga
    42421 atatttcgac aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg
    42481 cgcgccccag ttcccctgac catcaaccag cggataacgg taagagaacg gttgcgccat
    42541 caggaccatc gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc
    42601 accgacggta cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat
    42661 cgcatacaca atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg
    42721 gtccatgatc acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc
    42781 aaggcgctct gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa
    42841 aggggcaata ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg
    42901 tgaatagcgc cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac
    42961 aactctttgc tgtagacata atcctgggga ttatgcagcg gaaaaccgcc aacgtagggt
    43021 ttaagcagtt tgattaacgg ctgatagtag ataggaatca gcggtgcctg ttggttgatg
    43081 atcacttctg cctgctgata caacgcatta cgctttgtcg catcagtgat ctgcgtggcc
    43141 tggtttagta aggcgtcata ctgcgcattt ttccagtgac cgacgttttc ttcactatcg
    43201 cttttgagcg tgttcaggaa gctggaagca tcattgtacg tcgcatccca cgactgccga
    43261 gacagcatga aatcaccggc tcgtcgggca tcaagatagg ttttccactc cattgtgcgc
    43321 agcgtcacct gtgcacccag ccattttttc cattcggaag acaacgctat cgcggtcttt
    43381 tcatgcagat cgtacttgtt gtagaacagc tcaaagcgta gcggatgaga ggcgtcgtat
    43441 cccgcctgtt tcagcaaggc ttttgccatc gcgacgcgct cactcattgg cttttgcagt
    43501 tcatcgaacg tcgtcgcgct aaagcctttt acctctggcg gcgtcagcgt ggttgcgggc
    43561 gttctcaacc ccagtacctt ttgcgcaata agctgtcgat caaccgtaag atatagcgcc
    43621 cgacgcactc gcacatcgtt aaatggcggt ttctcaaggt tgaagttgta atattcgctg
    43681 ttcagacgcg gaataattcg tagctcgcca ggcagtgatt tttcaatggc gggaatttgc
    43741 tgcgccggaa cccaggtgag atcgacctct cccgcgcgat agcggttata gccggtgacc
    43801 gaattatcta gcgccagata ctcaacctgt tgcaatactg tatgttgcgc atcgcggtac
    43861 tttggatttt tgcgtgcagt aatcttttcg ttaactaccc actgatcaag cacaaaggca
    43921 ccgttgtaaa ccatgttctc tggcttactc cagctatcgc catgtttagc gatgacatga
    43981 tgaggaaccg ggaacagcgt cggccaggcg agcatcgtcg tgaaccaagg aaccggctgc
    44041 tcaagcgtaa cttcaagagt acgatcatcc gtcgctttga cacccagcga tgtaacatct
    44101 gctttacccg caacaatagc tgcggcattg ttaatgtgcg cctgtgccag atagccagca
    44161 aaagggcttg ccgttttcgg gtcaaccgcg cgctgccagc cgaggacaaa atcctctgcc
    44221 gtcagaggct gaccgtcgga ccactgcaaa ccgctacgca gatggaaaat atagcgcttg
    44281 ccgccgtcca gtatctccca gcgttcagcc tgagcgggct gcacctggcc ttcaccgtcc
    44341 atccatacca gaccttcaaa cagatccagc acaatctgcg cggcagtatt ctcctccacc
    44401 ttttgcgggt cgagcgtacc tgggtcgcta tgattgttgt aacgaaagac ttgttgcggg
    44461 gcgagcggtg tgttggcggg aacgtcagca gcgtagagag gagccgcact taccaggctg
    44521 accagccata ataaatttcg cgtatacatg gtattcctta ccggagaaat tataagccca
    44581 acaacaatac gcctgtggca ttgttcgctc aaacttatcg cgagtgattt ggctcacact
    44641 ccggtaaaga aaaaagcgag ttgcccccgc ttttccatta attaacggat ttcattcaat
    44701 agttctggat gcttatccag aacacgtaaa agtttgattg tggaaggatg aggttgggca
    44761 ttgccttttt cgtaacgcga aaacgcattt acacctcccc caaaaatttc gcttgcctct
    44821 ttttgggtaa gagagagctt ttttcgaacc ttcactataa attcaggtgc cactgtttcg
    44881 gcattcaccg aagcccgaaa tgcctttact tgcgccatga aagcatctga ctcttcttta
    44941 ttcatgatgc tctcttcgca atggacacaa tataaaccgt ggataccttt caatactgtt
    45001 tttcgtccac ggaaggtgta tggaatatct ttaatgccag aaaccatttc tccctggtgg
    45061 caaaccggac atttcatatt acttctcctt aaacgagacg atcagtacgt catgaattac
    45121 cgtaatttta agataaacct ggcctgtaac aagcctgggt ctgtaaacat cctgccagat
    45181 agtatgatca gagtaggtgg tcatgctttt ataaaagtcg ctctctgata atccaatgat
    45241 aacattacac ataccatcaa aatccaaacc taactcatct gcatttaaca gtgcactacg
    45301 tgttgtacga acttgcccgg cattgacaag ttttttaacc tgactcaaac gtgtatgtgg
    45361 tgtgcgtttt tccataaccc ccgcctccct gttactttag ttataaccta aaaggttaat
    45421 tacagcaatg aaaaagcacc taaaaggtta gttagatgta cggagatagt gaccacacaa
    45481 aacgtattct ttaaggaaag tgattgacca tataagaaag tggcgcatta gtagcgccag
    45541 tttgaagcag gaatttataa gggaagctgg agtcaggcat cacgccaacg gcacataaac
    45601 atccgtttcc agtcgctgct ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat
    45661 cggtgcttta cgcattttct cgccgcttgc aggcagccag tggcgaatga tgccccatac
    45721 cgtgtgtgaa atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccggt
    45781 aagttcacca ttgctcacac cataacgatt atcgggaatc ggttcgctaa cgctgccgca
    45841 gatatcaaag cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa
    45901 agtctggctt tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc
    45961 cacgctgtag ttgagcaatt cagggctaca gcggtgcggc aacatcgcca caggtattga
    46021 tgggaaatcg ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat
    46081 tattcccggg aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt
    46141 tgacgtcaat ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat
    46201 ctttcggcgt caccgtcacg ccgttccagc gtttgtggtc gatatcaaca ttgatagtac
    46261 cgctggcatc tttgaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca
    46321 gggttaccca ggtgtcgtca cgcagggatt ttgcgctttc tacagtcgtt acgctgccgt
    46381 tcggcccctg gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct
    46441 gctctgctgc catcaccggt gcgctgcaca gggccattac tgcgattact gctgcgaatt
    46501 ttttcatgtt tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta
    46561 acaacttctt aagggaaaaa aataaaattt agtgctgtac agagcgcgtt acaacacggt
    46621 ttactggcag caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag
    46681 atgacatgct gattggcgac ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg
    46741 actggtttac acaaggtcgt cagggaaaag aggcgcttta tagcgcacct tatgatgcgg
    46801 tgatcctgga tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag
    46861 aaaaaggtca gcgtgagccg gtactgatcc tgaccgcgcg cgatgcgctg gcggaacgtg
    46921 tagaagggct gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag
    46981 tcgccgccag gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc
    47041 gccacggtaa cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct
    47101 taacactgaa accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg
    47161 tactgccgcg caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta
    47221 atgccgttga agtgcatgtg catcatctgc gacgcaaact cggtagtgat tttattcgta
    47281 ccgtgcatgg tattggttac acattaggtg agaaatgaaa tttacccaac gtcttagtct
    47341 gcgcgtcagg ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag
    47401 ctttgtcgcc tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca cccaactgat
    47461 gctgtttgcc aagcggttaa gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat
    47521 ggcacagacg ccaaatagat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc
    47581 catctttacc cacgacggca gaatggtcct taatgatggc gataacggag aagatattcc
    47641 ctatagctat caacgggaag gttttgctga cgggcaactg gtcggtgaag acgatccttg
    47701 gcgttttgtc tggatgacct cacctgatgg caaatatcgc atcgttgttg gccaggaatg
    47761 ggaataccgt gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggctggt
    47821 cgcactgccg attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct
    47881 gaacaaactg gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc
    47941 gactggcgta cccagcgaag tgcggccact ggttgagtcg ctaaatcaac tgttcgcccg
    48001 cacacatgcg atgatggttc gtgaacgacg ctttacctcc gacgcagctc acgaacttcg
    48061 tagcccgtta acggcgctga aagtgcaaac cgaagttgcg cagctctctg acgatgatcc
    48121 gcaggcgcgg aaaaaagcac tgctccaatt acattccggg atcgatcgcg ctactcgtct
    48181 ggttgatcaa ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc
    48241 ggagatcccg cttgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca
    48301 gcaggcgaaa attgacgtgc gactgacact caatgcccac agcatcaaac gcaccgggca
    48361 accgctattg ctaaatttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc
    48421 acagggcagc gtggtagacg tcacgctgaa tgctgataat ttcatcgtga gggataacgg
    48481 ccccggtgtg acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg
    48541 acaaaccgct accggcagcg ggcttgggct atcgattgtc cagcgaatcg ccaaattgca
    48601 tggcatgaat gttgaatttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg
    48661 gtaacttctc tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa
    48721 atataaaccg tccccaaaaa agccaccaac cacaaaccag acaaataccg agcgagaata
    48781 catataccca aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa
    48841 gtggcgtaag aagaaaaaat gctgcaatca ttgcttgtaa tatacgatct tcgcctgaaa
    48901 cggaaggtat actgcaaata tttcc
//
LOCUS       DAZFZC010000026        48771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFZC010000026 DAZFZC010000000
VERSION     DAZFZC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.026"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(156..902)
                     /gene="cutC"
                     /locus_tag="ACHKXP_001730"
     CDS             complement(156..902)
                     /gene="cutC"
                     /locus_tag="ACHKXP_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO7646758.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(916..1482)
                     /gene="yecM"
                     /locus_tag="ACHKXP_001731"
     CDS             complement(916..1482)
                     /gene="yecM"
                     /locus_tag="ACHKXP_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7646759.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKLYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1698..3431
                     /gene="argS"
                     /locus_tag="ACHKXP_001732"
     CDS             1698..3431
                     /gene="argS"
                     /locus_tag="ACHKXP_001732"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO7646760.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVSEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3473..3517)
                     /gene="yecV"
                     /locus_tag="ACHKXP_001733"
     CDS             complement(3473..3517)
                     /gene="yecV"
                     /locus_tag="ACHKXP_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO7646761.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3608..4096
                     /gene="yecT"
                     /locus_tag="ACHKXP_001734"
     CDS             3608..4096
                     /gene="yecT"
                     /locus_tag="ACHKXP_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO7646762.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKYSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4217..4609)
                     /gene="flhe"
                     /locus_tag="ACHKXP_001735"
     CDS             complement(4217..4609)
                     /gene="flhe"
                     /locus_tag="ACHKXP_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO7646763.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4609..6687)
                     /gene="flhA"
                     /locus_tag="ACHKXP_001736"
     CDS             complement(4609..6687)
                     /gene="flhA"
                     /locus_tag="ACHKXP_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO7646764.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQPLQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6680..7828)
                     /gene="flhB"
                     /locus_tag="ACHKXP_001737"
     CDS             complement(6680..7828)
                     /gene="flhB"
                     /locus_tag="ACHKXP_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO7646765.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FYLWHHWPQMMRLMAESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8030..8674)
                     /gene="cheZ"
                     /locus_tag="ACHKXP_001738"
     CDS             complement(8030..8674)
                     /gene="cheZ"
                     /locus_tag="ACHKXP_001738"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO7646766.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8685..9074)
                     /gene="cheY"
                     /locus_tag="ACHKXP_001739"
     CDS             complement(8685..9074)
                     /gene="cheY"
                     /locus_tag="ACHKXP_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO7646767.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9089..10138)
                     /gene="cheB"
                     /locus_tag="ACHKXP_001740"
     CDS             complement(9089..10138)
                     /gene="cheB"
                     /locus_tag="ACHKXP_001740"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO7646768.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10141..11001)
                     /gene="cheR"
                     /locus_tag="ACHKXP_001741"
     CDS             complement(10141..11001)
                     /gene="cheR"
                     /locus_tag="ACHKXP_001741"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO7646769.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11020..12621)
                     /gene="tap"
                     /locus_tag="ACHKXP_001742"
     CDS             complement(11020..12621)
                     /gene="tap"
                     /locus_tag="ACHKXP_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO7646770.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEYEVARHESAQLQIAPVVS"
     gene            complement(12667..14328)
                     /gene="tar"
                     /locus_tag="ACHKXP_001743"
     CDS             complement(12667..14328)
                     /gene="tar"
                     /locus_tag="ACHKXP_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO7646771.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKRMAPLPEMVSTSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14473..14976)
                     /gene="cheW"
                     /locus_tag="ACHKXP_001744"
     CDS             complement(14473..14976)
                     /gene="cheW"
                     /locus_tag="ACHKXP_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO7646772.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14997..16961)
                     /gene="cheA"
                     /locus_tag="ACHKXP_001745"
     CDS             complement(14997..16961)
                     /gene="cheA"
                     /locus_tag="ACHKXP_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO7646773.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16966..17892)
                     /gene="motB"
                     /locus_tag="ACHKXP_001746"
     CDS             complement(16966..17892)
                     /gene="motB"
                     /locus_tag="ACHKXP_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO7646774.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLWKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17889..18776)
                     /gene="motA"
                     /locus_tag="ACHKXP_001747"
     CDS             complement(17889..18776)
                     /gene="motA"
                     /locus_tag="ACHKXP_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO7646775.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18903..19481)
                     /gene="flhC"
                     /locus_tag="ACHKXP_001748"
     CDS             complement(18903..19481)
                     /gene="flhC"
                     /locus_tag="ACHKXP_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO7646776.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19484..19834)
                     /gene="flhD"
                     /locus_tag="ACHKXP_001749"
     CDS             complement(19484..19834)
                     /gene="flhD"
                     /locus_tag="ACHKXP_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO7646777.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20614..21042
                     /gene="uspC"
                     /locus_tag="ACHKXP_001750"
     CDS             20614..21042
                     /gene="uspC"
                     /locus_tag="ACHKXP_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO7646778.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21049..22473)
                     /gene="otsA"
                     /locus_tag="ACHKXP_001751"
     CDS             complement(21049..22473)
                     /gene="otsA"
                     /locus_tag="ACHKXP_001751"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO7646779.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKIATFPKLA"
     gene            complement(22448..23248)
                     /gene="otsB"
                     /locus_tag="ACHKXP_001752"
     CDS             complement(22448..23248)
                     /gene="otsB"
                     /locus_tag="ACHKXP_001752"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO7646780.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23415..24401)
                     /gene="araH"
                     /locus_tag="ACHKXP_001753"
     CDS             complement(23415..24401)
                     /gene="araH"
                     /locus_tag="ACHKXP_001753"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO7646781.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24416..25930)
                     /gene="araG"
                     /locus_tag="ACHKXP_001754"
     CDS             complement(24416..25930)
                     /gene="araG"
                     /locus_tag="ACHKXP_001754"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO7646782.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(26000..26989)
                     /gene="araF"
                     /locus_tag="ACHKXP_001755"
     CDS             complement(26000..26989)
                     /gene="araF"
                     /locus_tag="ACHKXP_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO7646783.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            27786..28289
                     /gene="ftnB"
                     /locus_tag="ACHKXP_001756"
     CDS             27786..28289
                     /gene="ftnB"
                     /locus_tag="ACHKXP_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO7646784.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(28368..28619)
                     /gene="yecJ"
                     /locus_tag="ACHKXP_001757"
     CDS             complement(28368..28619)
                     /gene="yecJ"
                     /locus_tag="ACHKXP_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO7646785.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(28734..28820)
                     /gene="azuC"
                     /locus_tag="ACHKXP_001758"
     CDS             complement(28734..28820)
                     /gene="azuC"
                     /locus_tag="ACHKXP_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO7646786.1"
                     /translation="MKLRNILKSMFNNYCKTFKDVPPGNMFR"
     gene            29083..29406
                     /gene="yecR"
                     /locus_tag="ACHKXP_001759"
     CDS             29083..29406
                     /gene="yecR"
                     /locus_tag="ACHKXP_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO7646787.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            29577..30074
                     /gene="ftnA"
                     /locus_tag="ACHKXP_001760"
     CDS             29577..30074
                     /gene="ftnA"
                     /locus_tag="ACHKXP_001760"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO7646788.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(30112..30351)
                     /gene="yecH"
                     /locus_tag="ACHKXP_001761"
     CDS             complement(30112..30351)
                     /gene="yecH"
                     /locus_tag="ACHKXP_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO7646789.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            30542..31753
                     /gene="tyrP"
                     /locus_tag="ACHKXP_001762"
     CDS             30542..31753
                     /gene="tyrP"
                     /locus_tag="ACHKXP_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO7646790.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(31815..32480)
                     /gene="yecA"
                     /locus_tag="ACHKXP_001763"
     CDS             complement(31815..32480)
                     /gene="yecA"
                     /locus_tag="ACHKXP_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO7646791.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(32676..32762)
                     /locus_tag="ACHKXP_001764"
     tRNA            complement(32676..32762)
                     /locus_tag="ACHKXP_001764"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32726..32728),aa:Leu,seq:taa)
     gene            complement(32775..32848)
                     /locus_tag="ACHKXP_001765"
     tRNA            complement(32775..32848)
                     /locus_tag="ACHKXP_001765"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32814..32816),aa:Cys,seq:gca)
     gene            complement(32903..32978)
                     /locus_tag="ACHKXP_001766"
     tRNA            complement(32903..32978)
                     /locus_tag="ACHKXP_001766"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32943..32945),aa:Gly,seq:gcc)
     gene            complement(33130..33678)
                     /gene="pgsA"
                     /locus_tag="ACHKXP_001767"
     CDS             complement(33130..33678)
                     /gene="pgsA"
                     /locus_tag="ACHKXP_001767"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO7646792.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(33735..35567)
                     /gene="uvrC"
                     /locus_tag="ACHKXP_001768"
     CDS             complement(33735..35567)
                     /gene="uvrC"
                     /locus_tag="ACHKXP_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO7646793.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(35564..36220)
                     /gene="uvrY"
                     /locus_tag="ACHKXP_001769"
     CDS             complement(35564..36220)
                     /gene="uvrY"
                     /locus_tag="ACHKXP_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO7646794.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            36362..36478
                     /locus_tag="ACHKXP_001770"
     CDS             36362..36478
                     /locus_tag="ACHKXP_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7646795.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            36516..36692
                     /gene="yecU"
                     /locus_tag="ACHKXP_001771"
     CDS             36516..36692
                     /gene="yecU"
                     /locus_tag="ACHKXP_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO7646796.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            36679..36903
                     /gene="yecF"
                     /locus_tag="ACHKXP_001772"
     CDS             36679..36903
                     /gene="yecF"
                     /locus_tag="ACHKXP_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO7646797.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(36971..37693)
                     /gene="sdiA"
                     /locus_tag="ACHKXP_001773"
     CDS             complement(36971..37693)
                     /gene="sdiA"
                     /locus_tag="ACHKXP_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO7646798.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(37923..38675)
                     /gene="tcyN"
                     /locus_tag="ACHKXP_001774"
     CDS             complement(37923..38675)
                     /gene="tcyN"
                     /locus_tag="ACHKXP_001774"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO7646799.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTACSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(38672..39340)
                     /gene="tcyL"
                     /locus_tag="ACHKXP_001775"
     CDS             complement(38672..39340)
                     /gene="tcyL"
                     /locus_tag="ACHKXP_001775"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO7646800.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(39355..40341)
                     /gene="dcyD"
                     /locus_tag="ACHKXP_001776"
     CDS             complement(39355..40341)
                     /gene="dcyD"
                     /locus_tag="ACHKXP_001776"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO7646801.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(40446..41246)
                     /gene="tcyJ"
                     /locus_tag="ACHKXP_001777"
     CDS             complement(40446..41246)
                     /gene="tcyJ"
                     /locus_tag="ACHKXP_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7646802.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(41334..41885)
                     /gene="fliZ"
                     /locus_tag="ACHKXP_001778"
     CDS             complement(41334..41885)
                     /gene="fliZ"
                     /locus_tag="ACHKXP_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO7646803.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSNFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLAQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(41931..42650)
                     /gene="fliA"
                     /locus_tag="ACHKXP_001779"
     CDS             complement(41931..42650)
                     /gene="fliA"
                     /locus_tag="ACHKXP_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO7646804.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(42816..44309)
                     /locus_tag="ACHKXP_001780"
     CDS             complement(42816..44309)
                     /locus_tag="ACHKXP_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO7646805.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSSLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     KRDGNIMSDANGKLYVKVAGSDKSPENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            44564..45976
                     /gene="fliD"
                     /locus_tag="ACHKXP_001781"
     CDS             44564..45976
                     /gene="fliD"
                     /locus_tag="ACHKXP_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO7646806.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLGEISK
                     AINDADSGISASIVKVKDGDYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     ITTEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            45991..46401
                     /gene="fliS"
                     /locus_tag="ACHKXP_001782"
     CDS             45991..46401
                     /gene="fliS"
                     /locus_tag="ACHKXP_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO7646807.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEDSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            46401..46766
                     /gene="fliT"
                     /locus_tag="ACHKXP_001783"
     CDS             46401..46766
                     /gene="fliT"
                     /locus_tag="ACHKXP_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO7646808.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            46844..48331
                     /gene="amyA"
                     /locus_tag="ACHKXP_001784"
     CDS             46844..48331
                     /gene="amyA"
                     /locus_tag="ACHKXP_001784"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7646809.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFSARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEHWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(48365..>48771)
                     /gene="yedD"
                     /locus_tag="ACHKXP_001785"
     CDS             complement(48365..>48771)
                     /gene="yedD"
                     /locus_tag="ACHKXP_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO7646810.1"
                     /translation="LAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQRAL
                     VSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERDGNT
                     IEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT        12182 a        12791 c        11576 g        12222 t
ORIGIN      
        1 ttagcacaac gctatgcgca ctgtcacccg ctcaacagat cacggttatc gttcgttttt
       61 tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa
      121 gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg
      181 attcctttca tttcagcaac cgccgccccg tctacgacat aacgcgaata ctcgtccgcg
      241 tgtgcatctg atgacataga caatccttga ttacgataac gcatcggtga ggcttgccac
      301 gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttct
      361 gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata
      421 atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagcac ccgggcaatg
      481 cccaattccg caagattatt gagtgtattt agagggttag cgcacatatc aaaggcgcga
      541 tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg tggcatatcg
      601 acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa ttcgcggacc
      661 gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg
      721 cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga tttcagtacc
      781 cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac tctgtctgcg
      841 ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc cagtaatgcc
      901 atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa tggaccacgg
      961 atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg gtaatcgctc
     1021 atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga gtccttcatc
     1081 agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggca aaacgatttc
     1141 aatatgttcc caaccttcgt gtgggtaaag tttttccccg ggccacggta gctccacaat
     1201 ggaaaactgc cagtgcgcaa cctgtacagg ttcatgcaat ttaaacagac aaatcggtct
     1261 gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc gacgccagcg
     1321 ctcagcggtg acgttttgat ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat
     1381 attcaacccc agacggcggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga
     1441 tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc ttcgtctttc
     1501 agtaaaagcg ttaatttacc ctgttgccct gtgccaacca accgctgatt tcacgccgct
     1561 tctgatgcaa tagtgaaaac ggcaatacgc catgcgcacg ttgctgacga aaacagccat
     1621 ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg ctcgcttcat
     1681 caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca
     1741 tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc
     1801 agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatggcac
     1861 cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag
     1921 ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac
     1981 atgttcagca ggcgctggcg tccgatcgtc tcggtgttgc tacgccagaa aaacagacca
     2041 ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcacgtcggt cacctgcgct
     2101 ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac aaagtgattc
     2161 gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa
     2221 agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg
     2281 atgcgaaaaa gcattacgac gaagatgaag agttcgccga gcgtgcacgt aactacgtgg
     2341 taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca
     2401 ccatgacgca gaaccaaatc acctacgatc gtctcaacgt gacgctgacc cgtgatgacg
     2461 tgatgggcga aagcctctac aacccgatgc tgccaggaat tgtggccgat ctcaaagcca
     2521 aaggtctggc agtagaaagc gaaggcgcga ccgtcgtatt ccttgatgag tttaaaaaca
     2581 aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat ctctacacca
     2641 ccactgatat cgcctgtgcg aaatatcgtt atgaaacact gcatgccgat cgcgtgctgt
     2701 attacatcga ctcccgtcag catcaacacc tgatgcaggc atgggcgatc gtccgtaaag
     2761 caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg atgctgggca
     2821 aagacggcaa accgttcaaa acccgcgcgg gtggtacagt gaaactggcc gatctgctgg
     2881 atgaagccct ggaacgtgca cgccgtctgg tgtcagaaaa gaacccggat atgccagctg
     2941 acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat gcggatctct
     3001 ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg tttgagggta
     3061 ataccgcgcc atacatgcag tatgcataca cgcgtgtatt gtccgtgttc cgtaaagcag
     3121 aaattgacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat cgtgaagcgc
     3181 aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc cgtgaaggca
     3241 cgccgcatgt gatgtgtgct tacctgtacg atctggccgg tctgttctct ggcttctacg
     3301 agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt ctgaaactgg
     3361 cacaactgac agcgaagacg ctaaagctgg gtctggatac gctgggtatt gagacagtag
     3421 agcgtatgta atcgattttt cgtgagagta aagcctgatc agggttacct gatcaggctt
     3481 ttttattgcc atctaaatgt attctgaaaa tggacatgcc attgttttct gactgttgga
     3541 taagaggcca gaagcgtaat atccgacccc agggaaacga taacggttga atttaaggaa
     3601 taccgcagtg tttaaatttc ttgtattaac attaggcatt atctcttgcc aggcttacgc
     3661 agaagatacg gttatagtaa acgaccatga catttcagcc atcaaagatt gttggcaaaa
     3721 atattcagat gatgatactg acattaacgt gatcaaatca tgcctgcgac aagaatacaa
     3781 tctcgtcgat gcgcaattaa ataaagccta tggtgaagct tatcgttata tagaacaagt
     3841 gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt aacttgctta aaaaatcaca
     3901 gcgagcctgg ctggacttta gggacaaaga atgtgaatta atcctttcaa atgaggacgt
     3961 tcaggattta agtgaccctt attctgaatc agaatggcta tcatgtatga tcatacagac
     4021 caatacgcga actcgccagt tgcagctata ccgtaactct gaagattttt atccaagccc
     4081 tttgacaaga ggataattca catattttgg gcatgttttg ttgcaagcta ttcctgataa
     4141 atgattgcaa caaaacatcc agcctttttc actgagttat taaacatact cgcgagcgcg
     4201 taattttttt tgtccttcag cgataattca caatcacttc attccgttgt acctttagcg
     4261 gtggaattaa ccgcccaccg ccaggcactt cccagataaa gcgcaacggt tctgctgccg
     4321 ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca caacgagatt
     4381 gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga taacgccacg
     4441 ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca gacgacatcg
     4501 actcaccacg gtgattcagc gtaacgccca cactactcgc ctgccacatc ccctccccgg
     4561 cggcttgcac cagcagcgga aacaataata ttgctaataa ggctctcatt atttgccgcc
     4621 aatcgtcgcc gtcatgcgga tatgtcggtt atctgatagt tccagattcg acagcaccac
     4681 taactgcggc aagctgcggc gcagaaagcg agacaataat ggtcgcagcg cgtggttcac
     4741 caacaatact ggcggcgcac ccaacatctc ctgacgggat agcgcttcct gagtttgcgc
     4801 cagtaaacga tccgccaacc ctggttccag tccgcccccg ccctgcagcg gctgtagtaa
     4861 caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca
     4921 ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt catgtggatc
     4981 gctttggatg ggcgcatgtt ccgccagcgt ttcgagaata gtgcgcatat cgcgaatcgg
     5041 tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg taacgacgcc
     5101 aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca gcagctgttg
     5161 cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt
     5221 tgccaccacc gtgctggcct caaccacggt gtacccctga atctgcgcct gttcttttag
     5281 cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg cctcaccagg
     5341 taacgtcccg gcagcggtac cagggttaat cgccaaccaa cgccccggat aagcatcacc
     5401 gctgcctatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca gatccatatt
     5461 gtcgcgaatg tgcaccaccg gcggcagaaa ccccatctcc tgggcaaatt tcttgcgaat
     5521 actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg ggatcagtcg
     5581 ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg tcgcttcgac
     5641 aacggcatta ttctctgcca tttttaccgg ttttggttcg gcaggcgctt tttgttcgcg
     5701 tccacggatc caccaggcca gccccagcaa tcccgctgtg aacagtaaaa ataccaggtt
     5761 tggcattccc ggcaccagcc ccagtaaacc gagcactgcg gcacttaaca acatgacgct
     5821 ggggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg
     5881 cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg ccaccagacc
     5941 gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca tgccatgttg
     6001 cagcacaccg accagcaacc cgccgacaac gttaatgacc atgatgagga tcccggcgat
     6061 ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat cggcttcctg
     6121 agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccgg cgttaaggtc
     6181 ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg cacccacttc
     6241 tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga gaatgacaaa
     6301 caccacgata ccgatagcga aattgccacc aacgaggaag tgaccgaacg cttcgaccac
     6361 cttccctgcc gccgccgcgc cggtatgccc ttccattaaa atgatacggg ttgaagccac
     6421 gttaagtgcc agacgcaaca gcgtggtaaa caacagaatg gtcggaaacg cagcaaactc
     6481 aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa
     6541 ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaagatcaa
     6601 caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgcgggcag
     6661 gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt
     6721 ccggcaccgg aagatgagtc ggttgtacag ggcgctgtcc accagccaga cgccagcgtt
     6781 tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga cccgggattt
     6841 gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg
     6901 ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc aaccagccct gcacctttag
     6961 cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg
     7021 tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcgg
     7081 cagctcgctg catctgacgg atccgtcctt taacatgggg gtcgccttcg ctttgtttga
     7141 actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg aagatttgga
     7201 aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt gcgcatagcc
     7261 caaccagatc catcgcatta cccatggcgg tagtcggaga ctcggccatt aagcgcatca
     7321 tctgcggcca gtgatgccag agataaaacc ccgtcacgct gccaaccagg atggttttca
     7381 aaattgcctt aagcaactcc gcgccagtct gtgccgagaa catccgttta atgcccggta
     7441 gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc agccccccca
     7501 gcatgaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggc agcagcgcca
     7561 gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg tcattgataa
     7621 tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg gccagcgaca
     7681 caccgccaaa ccagataaca cacacgccca ctaacaaaat cagcaacgag gtcagttcac
     7741 gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt gtgggggctt
     7801 ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta ttaagcttaa
     7861 atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag gcgggattta
     7921 gggcttttgc tgccacacat caagcatagt gtgcgtttgt cggatgcggc gtcatcgcct
     7981 tatcagaccg cctgatatga cgtggtcacg ccacatcagg caatacaaat caaaatccaa
     8041 gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct ttgctggtat
     8101 cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac tcctgttccg
     8161 ggatgttttc caacagcacc atcagcaact ggcgttcgat ctcctgaatg acatccatca
     8221 tccgcttaat gacctgcccg gtgagatcct gaaaatcctg cgccatcatg atttccagca
     8281 gttgcgcgtt agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt cgtgtatctg
     8341 ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt
     8401 gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac gcctcaacac
     8461 tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac aaacgatcgc
     8521 gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc agttcccgca
     8581 aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat
     8641 gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat gcccagtttc
     8701 tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt caccacatag
     8761 ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc
     8821 attaacactg gcaatgccga catcgcgcca tccgcacgaa ttgttttcag caattccagg
     8881 ccatccatat tgggcatgtt ccagtcggag ataacaaatc cataaccgcc tgcctgcaac
     8941 ttattgagag cgtcgacgcc atcttccgct tcctcaacat tattgaatcc cagctctttc
     9001 agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt
     9061 tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg gcactaattt
     9121 ttgccaacat ttgctggctt acctggctaa gatcgaccac ttcgcagaca ccacccatat
     9181 tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc
     9241 atgcccccgc ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc ataccggtca
     9301 ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac aacacatcta
     9361 ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg taatttgcgc
     9421 cactacgcga cagctccata tgccgatcgc ccggcgcaat ataggcatgc cccggcaaga
     9481 cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta agtctgtcgg
     9541 caaaagagcg ggtgaaacca ggcggcatat gctgggtaat taacagtgcc gggctggaaa
     9601 gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa gcaccaatcg
     9661 caatcagttt ttcagaactc aacaacggcc ctgccttcag cgttgtcggt gccgacaatg
     9721 gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca atcatttcgc
     9781 tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa tctatcgccc
     9841 ccagctccag cgcgcgcagc gtaacttctg accctttgcc ggtcagggaa gaaaccatca
     9901 caacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg tccatccgcg
     9961 gcatttcaac atccagcgtc agcacatcgg gattgaattt cttaatcaag tcacgcgcga
    10021 ccagcggatc aggcgcggtc gccaccattt ccatgtcgct atggctgttg atgatttctg
    10081 tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt ttgctcatcg
    10141 ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc gctcaaggtg
    10201 gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa
    10261 gcggcgcaaa atctcctgct gggtattttg atcgaagtag atcatgacgt tacgacagaa
    10321 gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtagattca gcggggcaaa
    10381 atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc
    10441 tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt catggcgata
    10501 aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcactggcaa acactttcca
    10561 gcgtccgggc gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt acggctcttc
    10621 gccggttgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac gtgcgtgatc
    10681 cgcgagcaga gggaaatgat gagcctcacg gaaaaatgcc gtcagattcg tggtcagcga
    10741 attgataaac gcctgccact caccgctgtg ctgattagat tccagcaagt tcagataatg
    10801 accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc
    10861 gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac ttatccgccg
    10921 aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag acgtttgccc
    10981 acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca ctggcgcaat
    11041 ttgtaactgc gccgactcat gtcgtgctac ttcatattct tcaagggtaa ataccgccac
    11101 acgcgacgaa agatggtcgg cctgattcgc cagttgttcc gttgccaccg ccgcttcttc
    11161 taccagcgag gcgttctgct gagtcacctg atccatctgg ctgacagcct gtgcaacctg
    11221 ctcaatcccc cgctgttgtt cttccgacgc agaggcaatt tctcccataa tgtcgttcac
    11281 gcgggtcacc gaactgacaa tatcgatcat ggtcgcggcg gcgttattca ccagtttcga
    11341 accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt ttgctgcctg
    11401 cgcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac cacgcccctg
    11461 ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg tctggaaagc
    11521 aattccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtgg cgatctcctg
    11581 catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg tcgcggcatt
    11641 ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa
    11701 ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg tacgacttga
    11761 gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct ggcttccctt
    11821 acgcacatca cttaccgtcc cacgcaaagc ctgctgcatg gtcttcagac tggcaaaaat
    11881 ggcggtgatc tcattacgac catataccgc aatcggacgc gccagattac ccgcagcaat
    11941 gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc gcgtccacca
    12001 cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca ccagtgccga
    12061 aatctgatag ttacgctggc tttgcgcact ggcggcttcc agcacatggt tgatctccag
    12121 ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag
    12181 gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca
    12241 gcgggcaaaa cttttctccg tctctttttg caatgccctg acgtgctcgt tgcccgcagt
    12301 catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt
    12361 catcaacgtt ttaatgtcat ccgccggata gctaagtgcc gtcagagtgc ccgctttgtt
    12421 cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg cacgttgctg
    12481 attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat
    12541 gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca gcgtggtcga
    12601 aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa
    12661 tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc gtggctgtgc
    12721 cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga gtgggctggc
    12781 tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt ccagcgcagc
    12841 ggcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc
    12901 cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg ccgatgcaat
    12961 ctcgcccata atgtcagtca cgcgagtgac agcattgacg atattgttca ttgtttcccc
    13021 ggcgctttcg accagcaccg aaccggtatc aacgcgtgcg acggagtctt caatgagggc
    13081 tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt cacccgccac
    13141 cacggcaaaa ccacggccct gttcacccgc acgcgcggct tcaaccgcgg cattcagcgc
    13201 gaggatatta gtctggaagg caataccgtc gataacgctg ataatgtcgg caattttctt
    13261 cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt tgccgccgtg
    13321 ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt
    13381 ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca gcgcggatgc
    13441 ctgctgttca gtacgggagg aaagatcggt gttgcccgcc gcaatttcac gggtaccggc
    13501 atagatggca tctgaacctt cgcggacatg agtgacggtg tcagtcaaag agcgttgcat
    13561 atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg tcagggtatt
    13621 cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca gcggagtaag
    13681 caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca ccagcgcgat
    13741 aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga cgatatcgcg
    13801 atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg cattttgcat
    13861 tccctgggtt ggctgagcga aataagctcc agtattgcca taatctagat aatcaatcag
    13921 ttcagttaac gctgtgtaat agtttttata tttttcatca atattacgac tggtagagac
    13981 catttcaggt aacggtgcca ttcttttgaa ttttttataa tgcgtcgctg cctgcgccaa
    14041 tgttttcctg gcgctatcga gcaattcaac tttggcgtta ctttgttgat tggaggaatc
    14101 catcatcatc cgcaccgctg aacgactcag gttaatgcgc gtttgcagca ttaaatccca
    14161 ggttgacgtc agctcgccct gctgttcccg caattgattg gaaaccacaa agctcttctg
    14221 gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca gtgcgaatac
    14281 ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa ggcaccttcc
    14341 tgaaaacaag ttgatctcgt tatcggcaag aaggggggaa actttattgc tgatgccacc
    14401 cgccgcgaaa ttgaaataaa aaacccgatg cgcagatcat cgggttcatt tcaattgagg
    14461 aaatcgggag aattacgcca cttctgacgc cgcgctatct aacagcgcca tctcttcgct
    14521 gttcagcagt ttttcgatgt tcaccagaat caacatccgg tcgcccagtg cgcccagtcc
    14581 agtgagatat tctgttgaaa gcgtcacggc aaattccggt gccggacgaa tttgctccgc
    14641 cgtcaatgaa agcacgtctg agacgccgtc aaccacgatg ccgaccaccc gctgtccgag
    14701 attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc tgaacttaat
    14761 tcgtaagtca acaatcggca caataacgcc gcgcagattc gtgacgcctt tgataaacgc
    14821 tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca ctttcaggat
    14881 atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc ctgacggctc
    14941 gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt actcattcag
    15001 gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca
    15061 atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg gactttgcga
    15121 tagttacttt caaggttttt aaccacaacc tggtgctgac caattaattg atccaccagc
    15181 aaggcgtagc ggcgaccgcc actttgtaag atcaccacaa ttccctgggt ggcttcggtt
    15241 ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata ttcgccccgc
    15301 acttccagca cccgctcgcc gccggccagt ggatgcagat cggcttcacg gggttgcagt
    15361 gattccatga cagcattcag cggcagaatg aaaacttcat ccgcaacgcg tacggacatg
    15421 ccgtcgagga tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc agtaccctgc
    15481 ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac gacgtccatg
    15541 ccgacgccgc gcccggagac gtcggtgacc tgctctgccg tggagaagcc tggtgcaaat
    15601 atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cagtcaaacc ttgcgaggcc
    15661 gcttttgcca gaattcgctc acggtttagc cccgccccat cgtcggtcac ttcaatgcaa
    15721 atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt tttacctgcg
    15781 gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac caggtgggtc
    15841 agcgggtcga taatgcgttc tatcaggctc ttgtcgagct cagtggaact gcccaccagc
    15901 gtcagttcta cctgcttgcc gagttttccc gccagatcac gcaccagccg gggatagcga
    15961 ctaaaaacat attccatcgg catcatgcga atcgacatca ctgattcctg caaatcacgg
    16021 gcgttacgtt gtaactgccc catgctggtt atcaaatcac catgattaac cgggtccagt
    16081 tcgctggaac gctgggcaag catggactgg gtgataacca gctcgccgac gaggttaatt
    16141 aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct gcgcgtcgtt
    16201 ttttcccgct ccacgcggcc ggttggcgct tgttcagctg ccagtttaag cactggtggg
    16261 gtggatattt ttggcgagac ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca
    16321 aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat tgccgagagc
    16381 gaatccgccc ctttcaccac gtcagttaac gttgtcagat gtcccagttc ttcttccagc
    16441 aggtcgactt ccccggcctt caggcgcgaa aggataattc gtcgcggcga ctgactgcga
    16501 ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac tgcggatggc
    16561 gtttcgcctt tcgcttctaa tgccaattga cgcaaggcct ggcagatata atcgaagctg
    16621 gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat gatgtccttc
    16681 gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg tctggcttca
    16741 tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc aaaagttcct
    16801 gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc ggcatctggc
    16861 gcttccggct gcaaaaccag cagatgctgc tccatatcag ccaacagttc gtccgcttca
    16921 tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc tcggttcggc
    16981 tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca gggcgcttac
    17041 tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt gtttgttcag
    17101 taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat
    17161 cgttgccgcc atgccgacga cacgtaacac tttgccgcta tccaaccctc cgaccatcag
    17221 ttcgcggcgg gatgcattgg cccgatcggc agaaagctcc cagttgctgt aacctttctc
    17281 accactggcg taggggaaat catcggtatg acccgaaagg ctaatacggt tgggaatacc
    17341 attcagcaca ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga catcggcact
    17401 gccggttcta aacatcgggc gattctggct atcgatgatc tgaatacgta gaccttcctg
    17461 gaccagatcg attttgagat ggggacgtaa cgcccgcagt ttcggatcgg actctatcaa
    17521 ctggtcgaga tcaccccgca atttccacaa tcgactttgc tccatgcgtt ttttcagctc
    17581 ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat caccaccgcc
    17641 gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag tcgccagtgg
    17701 agtccggaag tactccgcaa tctgaatcaa ctcttttggg ctggagatgg agatcagcca
    17761 catcaccaga aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa tcttccacga
    17821 tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc
    17881 ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca
    17941 tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt gcgaccaaac
    18001 tcaacggcga taggcggtgc gtaaccgttc agattagaaa gcagagtgac tttgacgcac
    18061 tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cactcgctag tggggaaata
    18121 aatccgtaag ccaataaaat accgaggaaa gtccccacca tcgcatgtgc gataagcgcc
    18181 cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc cattacagcc
    18241 gcgacaatac caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt cgccgggact
    18301 tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gggcttcgat ttcgaaggtg
    18361 ttcatgtgac cgctgataat caggcgcaga taatcgacga taaaatcaag catgacgcta
    18421 tccgcgagga tgcgtgggta gctggcgaag atctcgctct cacggggatt ttcaatatca
    18481 cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata aagcagagcc
    18541 agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa cggcagcgct
    18601 ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc aatccctgca
    18661 ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc accggtcatc
    18721 aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat aagcacgaca
    18781 tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa cgcgcgattg
    18841 gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgttgcgcc acaccgtatc
    18901 agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc ggcaggattc
    18961 tgggaaagtt tacgtctttt tactgcccgg gatggcggtt gacataagct gcaggcaaag
    19021 ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt gcagctggaa
    19081 agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt taatgccagc
    19141 agtggtcctt cttctgcttg tgggcactgt tcaaggtata aacggtaggc tttgatcacc
    19201 gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcatt acagaacatc
    19261 gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct
    19321 ttcggcggtg ggcttccgcg cagttcttta taaagtttta tcaggcgtcc gcgacttaac
    19381 tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc cattgccagc
    19441 tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc cttttcttgc
    19501 gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc atgatgccgg
    19561 tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta atcgtctggt
    19621 ggctgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta accatttgcg
    19681 gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga cgaaacatag
    19741 cggacgcttt gtcctgaaca atcaaacgct gtgcaagtag taaatatgac aagttgatgt
    19801 cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat aaccaacttt
    19861 atttttatgc ggtttcaccg caccccgtga tgtcgccggg aagacccggc aaaaaataat
    19921 tagcattaga atagttgcga taagctgcaa taagcagagc cacctttttg gtttaataag
    19981 tccttacaaa tagaaggggg tctttacact tatctaagat ttttcctaaa tcgacgcaac
    20041 tgtactcgtc actacacgca catactacgg aggagggctg cgattttcaa taatgtgtga
    20101 tgcagatcac acaaaacact caattactta acataaatgt ataagcaata cttttgtttt
    20161 gggtgtattt caatctgtta aaaagttttt cgctacgcta gcaagcaaaa atgaaacagg
    20221 aataatcgaa atgggatgtt gcgcacagtt aaaataactc accgtaaata atcatctgct
    20281 ataaataatc actttcatgc aataccagat aagctatttt taaacagaca cttaccgcac
    20341 aacaaactaa ttagatacaa agaaaggcaa caatcaactt aatgttaata gaatgttttg
    20401 catatattaa ccacttgttt aatacgggga atcagtacat tattttctta taattacagt
    20461 tgaaatcata tgatcaccgg gagtgattga ttaattgtct ttactaataa tcagactaat
    20521 atttacctgt ttgactgagt tgggattgcg tcgtttctcc attaggagta aagctttaat
    20581 gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat tcttgtcgct
    20641 gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat cgccaggcca
    20701 gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta caatcaatta
    20761 gctgcgccga tgcttgaaga tttacgcagc gtgatgcagg aagaaacgca aagctttctt
    20821 gataagttaa ttcaggatgc ggggtatccc gttgacaaga cgtttattgc ctacggtgag
    20881 ttaagcgaac atattctgga agtatgtcac aaacatcatt tcgatttggt gatttgcggt
    20941 aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt gattgcctca
    21001 agtgaggtcg atgtgctgtt agttccactt acgggagatt aaccgctcct atgcaagctt
    21061 tggaaaggta gcaattttat cgcgctgctg gctttccgcg cttcgcggaa ctatctgctt
    21121 taggtcgcta atgaagcact cctgccagtg gttaatatcg tttttcacga taacgtccag
    21181 catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac gatccagcgc
    21241 agctgcaact tcgtcacgat cgtaggggtt aacaattaac gccgacgtta actcgtttgc
    21301 cgctcccgca aattgcgaaa gaacaagaac gcccgggttg gctgggtcct gagcagcaac
    21361 atactctttt gctaccaggt tcatcccgtc acgcagtggc gtcactaagc ccacgtcaga
    21421 gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat aataaagcgg
    21481 cgtccagcct aattgcccgt atttaccatt aattcgtcca gcttcatttt cgagctgatg
    21541 acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct gggtataacg
    21601 aattttacca tgatgctgcg gatatttttc cagcaacgct tcataggcga gaaaacgctc
    21661 tggcaaacct ttggaataat ccagccgttc gacagaaaag atattttgta cgtttttcag
    21721 ttccgcttta agttgcgcca gttttggcgg cagtggcccg gcagcctgtt tggctatttc
    21781 tttcggttca atgccgatcg ggtagacttc tgttcgaaat gctttgcccc aggctgtatg
    21841 gctttttgcg ctacgtgtcg tgacgcgggt caggttagaa aggcaatcca ggaacgccag
    21901 acgatcgttt tctgtctgga aacccagcaa atcataatca caaagctgtt caagcaaggt
    21961 gtcatatgtc ggcagcgcgt tgaagatttc cggtgtcggg aaaggaatat gcagaaagaa
    22021 accaatgcga ttattcactc cccgtttgcg taattcatgc gcaaagggca acaggtgata
    22081 atcgtggatc cagataatgt catcgtcttg caacagcggc agtaatttat ctgccagcaa
    22141 cgcatttacg cgtagatagc cgtcccaggc aggacgctga aattgcacca gatcgagccg
    22201 ataatgaaaa gcgggccaga gaacggcatt ggagaattgg ttgtagtatt cgtcaaggtc
    22261 ctgttcgctg aggttaaaag aggcccacgt aatgttacct tttttcacct tttttagcgg
    22321 ctgatcctca ttccctgttt caccactcca gccaaaccac agtccgcctg cggctttcag
    22381 tgcccccagt atgccaacgg caaggccacc ggcactggcg gcgtgctcgt ctggtggtgc
    22441 aatccggtta gatactacga ctaaacgact catagtcatc actcctgtta ttttctcttt
    22501 tttgttgtaa tgcggtggtt atcatttcaa gccagctcca gacatccggc acacccgcca
    22561 gtcgccatga tgcctgagtt gcacctgtgc caatttttac tgacattccg cccagtcggt
    22621 taacgactgc gaagccagat tcatcggtta aatcatcgcc cagaaatacg ggcgttcgcc
    22681 cgataaaggg agcttcctgc ataaaagctg caattgcctc acctttactg gtacctctcg
    22741 gtttgatctc gacaacacac tttccctgct gtaacgccat ttgtggccag atctgagtaa
    22801 tacgttgcgc taatgtcatt aatgcgtctt catgctgcgg agcctgacga taatgcagcg
    22861 caaaagccat ccctttcgcc tccagctccg cgccgggata ctgagcgatg actgtatgca
    22921 gttgcacgct aatatcacgc gcaatcgcat ccggcagatg aacgatatgt gttttaccat
    22981 tgatgtcacg gcgctctgcc ccatgcacgc ccgctaacgg gaagcgataa ggttttgcca
    23041 gtgcgtcaag ctccaccatt gagcgccctg atatcaatgc caatgcacca tcacttgcgg
    23101 ttgccagtag ctgaagtcct tgcagaatat tgtcaggcac aacgacctga tcgggatgcg
    23161 gtttgatttc cgccagcgtt ccatcgagat caaaaaacca ggcatatttc gcggatagtt
    23221 caggggtttc ggttaacggt tctgtcaccc ggttctcctc cttctctttt cttattgttt
    23281 tgcgcagaac gcgctttaca gacatgtaag tatagacagt gtgacggggg tcgccatttg
    23341 caacgaagaa cagccaaccg ttatggcggt tggctgtggt gggaaaaaac gctaaattgt
    23401 tgcagaaaaa agcatcagac agtgcgtttc gctttttgct tgtaacggtc gaagatcacc
    23461 gctgccagca ggattaagcc gcgaaccacg tactgcgcga aaggagaaat attaagcagg
    23521 ttcatggcgt tttccacggt gcctaaaatt aagatacccg ccaccacata tgagattttt
    23581 ccgatgcctc ctttcagaga aacgccacct aaaacgcagg cggagataac aatcagctcg
    23641 taaccaatcg acgtcattgg ctgcccactg gtcatacgtg aagccagaat aattccggct
    23701 atcgcagata ccaggccaga gagaacaaag ataataattt tggtgcgaac aaccggtaca
    23761 cccgccagac gcgcagcctc ttcgttcccg ccaatcgcca gggtgttacg accaaaggtg
    23821 gttttattca gcaacaaacc aaagataatc agacacgcga cggtgagcca gattggcgca
    23881 ggcagaccga accagttagc gtaaccaagg gcaaagaagc tttcatcttc gataccgacc
    23941 gctttaccgt ctgaaatgat gtacgccaga cctcgaacaa tctgcatcgt tgccagtgtt
    24001 gtgatcagag catttatttt cagtttggcg ataacaaagc cattgaccag gccacagaga
    24061 atgccaagca gcaaccccgc tgccacgcca atccacaggc tttcagtcag gttgataacc
    24121 accgccgtgg tgacacccgc acaggcaatt acggaggcga cagaaaggtc aaagtcaccg
    24181 gaagcaaggc agaacaacat gccacacgcc accatccccg acatggaaat tgccaggccc
    24241 aaccctttca tattaatgaa ggtggcaaaa tttgggacaa aaatggcaca ggcgataaag
    24301 agcaccgcaa acaccaccag catgccgtac tgatcccaga tacgcccgaa gctgaatgac
    24361 gacttaggtg cgccagaccc cgatgtagaa acagaagaca tcatactctc cttactcagg
    24421 caactgcctg gctgacttta ggcatcgcaa ggctcagtgc ctgacgctca tctgcctgct
    24481 cgtgtaacaa ttcaccggcg atttcacctt cccgcatcac cacaatccgg tcggcaacgc
    24541 cgaggacttc aggtaagtcg ctggaggcaa acagcaccgc cacaccctgc gccgccagcg
    24601 cataaatcac gttgtaaatt tcatgcttag caccaacatc aatgccgcgc gtaggttcat
    24661 ccagcaaaat gaccttcatc tcttccgata accagcggcc cagaatggct ttttgctgat
    24721 ttccgcctga gagattcatg atcagttgct cagcgcccgg cgttttgatg ttgagcgaac
    24781 gaatgtggtg atcggcattg ttttcttccc aaccgttgtt gattacacaa ccgccgagca
    24841 catgtttacg tctggcactg atgttgatat tgtcgcgaac ggagtgcacg ggaataatgc
    24901 cttccgcttt gcgatcttcc gggcagagca tcatgcctgc ggcaatggcg tggctcggtt
    24961 tacgaatatc gatcggctgt tggtcgatat aaacctgacc ggcggtgatt tgcgtcccgc
    25021 caaacaagcc tttcattaat tcgctacgcc ccgcccctac cagaccaaac agcccaacaa
    25081 tttcaccact gcgaaccgcc agacttattg gcgtacgcac gcctggtgct ttcacagcat
    25141 caagacgcag gcgctcctcg ccataactac gaggttgcca gccgtagata tcgccaatgt
    25201 cgcgcccgac catcgcctgc accagcgcgt cgtggtcaac ctgctgcata tcggtaaagg
    25261 ttttgacata acgtccatct ttaaagacag tgatggcatc gctgagggca aatatttctt
    25321 ccatacggtg agaaacgtat aagatcaccc gcccctcttt tcgcagttca cgaataacgc
    25381 ggaaaagatt gtcgatttcg cgggcagaaa gggagctggt tggctcatca aaggcgataa
    25441 ttttagcgtt acgcgccagc gccttggcga tttcaaccat ctgccactga ccaatggaga
    25501 gatatttcag cggcgtgtcc gggtcaatat ccataccaag atgtttaagt tgtaaacccg
    25561 cctcataatt cagcaatgag cgattcacaa tgccgccttt atgcggcagc tggccgagat
    25621 agatgttttc cgcgacggtc atttccggca cgagatgcag ttcctggtaa ataatcgcca
    25681 cacccgcgtt aagtgctgcg gtcgtgtcgg aaaaggacat ttcctgccca ttaatcacta
    25741 cagaacccgt ggttggcgca tagttgccgc tgaggatttt taagagagtt gattttcctg
    25801 cgccattttc acccatcaac gcatgaacct gaccggcata gcagtcaaaa ctaatatccg
    25861 tcagcgcctt aacgccggga aacgttttac cgatgccgcg aaatgagaga tacggggtag
    25921 actgttgcat aacgtctccg tgaatcactg gtcgttcaca ccccctctgc atcatgcaga
    25981 ggggaatttt tccggcaaat tacttaccgc ctaaaccttt tttctccagt tcttctttaa
    26041 agttgtcacg cgtgatcagc accacgtcgg taacttcggt aaattttggc ggttcaacgt
    26101 cttttgctac ccagttgtaa agcatttcgc tggatttata gccatgtacg tccgggcttg
    26161 gcagcaggga accgtagaag ccggttgcct gtgctttaga cagttcgctc accgcatcca
    26221 caccgttaat gccaatgccg atgatatcgg cagctttaaa gccctgacct tccgtcgcgc
    26281 gtacgccgcc cagcacggtg ctgtcgttca taccgacgat cagccaatgt ttaacttccg
    26341 gatgttgaac cagcattgag ttggcagcgt caaatgcccc cgggatgtcg ttagatttgg
    26401 taggtacctg ataaatttgt ttttccggga atccggccgc tttcagcgca tccatagaac
    26461 ccgttgtacg gcgacgtgcc gtatccagtt cgttggcggt aatcgccatc accgcgcttt
    26521 ctttgacatc ccagccacgt ttctgcatct ctttatacag ttcctgaccc tgacgttcgc
    26581 caattttagt cgccgccatc atcaccagcg gaacggtatc cattggctta cctttggcgt
    26641 taacaaactg gtcatccacg gcaatgactt tcatatcgta gccacgcgct ttcgcgacga
    26701 tggcagagcc gagtttgggg tccggagtac aaataacgaa accttttgcg ccactggcag
    26761 ccaggctgtc gatcgcgttc aatgtttttt cgccatccgg cacggcaatc ttaataacct
    26821 caaaccctaa atccttcccg gctttatcgg caaacttcca ttcggtctgg aaccacggct
    26881 cttccggttg cttcaccaga aaaccgagct tcaggttctc cgccatagcg gattgtgaca
    26941 taacggctgc cagaccaatg gctgccaggg ctttagtaaa tttgtgcatg gttctctcca
    27001 gctttagtgt cgttttgtgt agggcaaaaa cgaatgactt tcgttaaatt aatcggaaaa
    27061 caaagcatta ccttttatct aaaagataag tgaccgtgtt gacatagttt tagcgagaaa
    27121 ttaattctcc ataggagagc aatatcacat cacagaatta cagtgagaac gtgcataaat
    27181 ttagcgggaa aagacataag ggaaagccaa tttgtcagac aaattgtcga atgcacagca
    27241 gattaatcca taagattagc ctggaaatcc ttgttgtctt tggtacccat gcgggatgtc
    27301 ttctttttaa ccagtcaata ggccgcatta cctggcgttg agtttttgaa atggtgtaat
    27361 aaccgcaact caaagatgtg gaaaatgcac gtcattcatt tcgccattaa ttatcactgt
    27421 gctcattaat taacagaaca cgtataatga gagccatctc gcaaaaatga aaaaacgttt
    27481 tataaaatca tcacttcatc atgaattcaa attcattgat taatatcaac aagatacaaa
    27541 aagcactatc attaaaattc attgcagtta cattgatttc atcaatgaaa tgtaaaaata
    27601 tataaacttg atgatttaag cattttctta tacccgttca gacgttattc ttatttctga
    27661 tcatcgtcag aatagactcc acgatcacat ttcggaccga cagaaaggaa ttattctgca
    27721 aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat
    27781 taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt
    27841 acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca
    27901 ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta
    27961 actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa
    28021 aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta
    28081 gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt
    28141 tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg
    28201 atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc
    28261 tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat tgcgccgatg
    28321 aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca
    28381 ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc ccgcatggtg
    28441 accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg actggacatt
    28501 ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc
    28561 aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc
    28621 gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata
    28681 gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg
    28741 aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc
    28801 aggatgttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattgtct
    28861 ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata
    28921 aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat ttctgacacc
    28981 tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt
    29041 aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact tctgattctg
    29101 actctttcat taataacgct ggcggggtgt acggttactc gccaagccca tgtgagcgaa
    29161 gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc
    29221 cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat
    29281 acccacgcga tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta
    29341 tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt
    29401 ctctaaatat tttaatcagc gaggggatct tcgctgatta aataaatagc ggtaatgcgt
    29461 tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg caaaaaataa
    29521 aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc actatcatgc
    29581 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac
    29641 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
    29701 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattacctga
    29761 ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
    29821 cacttgatga attattccag gaaacctata aacacgaaca attaatcacc cagaaaatta
    29881 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcaat
    29941 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
    30001 gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc tctaccctcg
    30061 acacacaaaa ctaatgctct ccggcggcag gtttactgcc gccgtttcaa atcagtgacg
    30121 gcaaatctta ctctgatcgg tcgaaaaacc gtctttcgaa ggtataaatt tgccttttgc
    30181 tgccagaaac gctaccagct ctcccgctgt catcccttct gccgagcagg tgtgaaaacg
    30241 tgcctgttca ccaaaacgcg ctttaatcgc agcttccaga ctggcatgcg tatattgctc
    30301 gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca tcgtccctcc
    30361 tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag cgcaggactg
    30421 aatgcttttt attgtacatt tatatttaca ccatatgtaa cgtcggtttg acgaagcagc
    30481 cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag
    30541 cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg
    30601 catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt taatcttgtt
    30661 gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg tgtaccagca
    30721 tgttccggca gataccggtc tgggcacgct ggcaaaacgc tatctgggac gctacggtca
    30781 atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg catacatcag
    30841 cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc
    30901 aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg tcgggacatc
    30961 gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc tggtggtgat
    31021 gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca
    31081 ggggctggct ctgtcagcaa tcccggtgat ttttacctca tttggttttc acggtagcgt
    31141 gccgagtatt gtcagctata tggatggcaa cgttcgtaag ctacgctggg tgtttataac
    31201 cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcaacgc ttggcagcat
    31261 tgattcaaca acctttatgg gattgctggc taatcatgct ggattaaacg ggctgttaca
    31321 ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt tatttgctga
    31381 tttagccctc gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt atctggctga
    31441 tttatttcag cgttcaaata ccgttggtgg acggttgcaa actggtgcaa ttacctttct
    31501 gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc
    31561 cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct ggcaaagcag
    31621 aaagcacaat cctcaggcgg gttaccgggt gaaaggtggt cgtccggcgc tggtggtggt
    31681 gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag ggttgttacc
    31741 agaagtgggg tgatcagata gcctcaaatt ccttattggg tgccagaatt aacgctgaca
    31801 cccaatttga cctcttaatg caggcagcac tgcttaaatt tcttaccact accgcacggg
    31861 caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac agctttttcc
    31921 tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat ggcatctacg
    31981 ctctcttcaa acgcttctgg cgacattttc tctacccgct cgaagttttc ctcagtaccg
    32041 tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg gcaacgttga ccagtcagaa
    32101 agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag ctcgctgcca
    32161 tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt aagacgctct
    32221 gcggtatcgg ccatatgttg aaaagccaga ttcataaagc gcgtcatctc tttctctgac
    32281 gcccagcgcg gcacatagtc agccccaccc cacacggcaa ccagccactg ttccggttca
    32341 atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc cacatcaagg
    32401 atggcgtggt cagtgttgta tttggtcaga atatcgtcca gccattccaa ctcactttcg
    32461 tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca gcacctatct
    32521 tacatgccgg tccgtatcag agatactttt tgagtggctt tgccggtgat taaaaattaa
    32581 ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt ttcacgacac
    32641 tgcttattgc tttgatttta ttcttatctt tcccatggta cccggagcgg gacttgaacc
    32701 cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt ctaccgattc caccatccgg
    32761 gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg gactagacgg atttgcaatc
    32821 cgctacataa ccgctttgtt aacgcgccaa attcttcagg cctttcagcc agacatccgc
    32881 ttgaggccga tgtcttttaa actggagcgg gaaacgagac tcgaactcgc gaccccgacc
    32941 ttggcaaggt cgtgctctac caactgagct attcccgcat tcatcaagca atcagttaat
    33001 cacttgattt tattatcgtc tggcaatcag tgccgccgtt cgatgcgttg cattctactt
    33061 acctggcgcg atgagtcaac gatatttttc accacttttg atcgtttgct gaaaattgcg
    33121 ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg ctcaaatatt gcaacattga
    33181 ccacagagtc agtaccgcag ccacaaagaa aagtgcaata ccggcgtact caacccaaat
    33241 gttcggacgc cacagcagcc aggccaacgc caccatctgg gcagtggttt tcactttccc
    33301 aatccaggag acagccacgc tactgcgttt acccaactcc gccatccatt cgcgtagcgc
    33361 agaaataata atttcacggg cgatcatcgt tgccgccggt agggtcaccc accagctgtg
    33421 ataatgctcg gttaccagca ccatggcgat agccacgaga actttatctg ctacagggtc
    33481 aaggaaagca ccaaaccggg tactctggtt ccagcggcgt gccagaaaac catcgaacca
    33541 gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca aacggcgacc aggtgacagg
    33601 cagataaaag accaatacaa agaatgggat aaggatgaca cggaacagtg taagcaacgt
    33661 agggatatta aattgcataa tgacgggtaa ctatctgttg tcagtaagat tacccctatg
    33721 ttgctacaga gacatcaatg tttcaacgac cagaagatct tttctgccag accttgcgaa
    33781 atacccggca cttttgcaat ttcctcgacg ctggcgttac gtaaaccttg caaaccgccc
    33841 atatatttca acaacatttg ccgacgtttt ggcccgacgc cttcaatggt ttccagggaa
    33901 ctggtatttt tgactttcgc ccgtttttta cggtgcccgc caatcgcgtg atcatgtgat
    33961 tcatcgcgaa tatgctggat aacatgcagc gcgggtgaat ctggcggcaa actaaatccc
    34021 tcaccttccg gctcaaagaa cagcgtttcc agtccagcct tacgatctgc acctttggca
    34081 acgccaagta gcagcggatg atttttatcc catgagacat ccagttcggc gaagacattt
    34141 ttcgcctgcg caagctggcc tttgccgccg tcgataagga tcacatccgg gatcttactg
    34201 tcgtcaatgg ctttaccata acgccgacgc agcacctgat tcatcgccgc ataatcatcg
    34261 cccggcgtga tgcctgtaat gttatagcgc cgatactccg cacgcagcgg gccgttagca
    34321 tcaaacacca cacaggaagc gacggtttgt tcgcccatgg tatggctgat gtcaaagcac
    34381 tccatccgct tcacttccgg caatttcaac acgctggcaa gcgcggtcag ccgctggtga
    34441 acggtagatt gctgcgaaag tttgctggtt aaggccgtcg ccgcattggt gcgcgcgagt
    34501 ttcagataac gcgccctatc gccgcgaggt ttggtttgaa cattaatctt gcgtcccgcc
    34561 agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa gattaaaatc gagcaggatc
    34621 tcacccggta aggtgcgcat ctggctgcct tgtaaataga actgacctac gaaggtttct
    34681 accacctcgc tcagttccgt accgccaggc actttcggga aatagctgcg gctgccgagc
    34741 actttgccct gacgaatgaa caatacgtgg acacaagcca tgcccgcatc gaacgccaca
    34801 ccaataacgt caaggtcgtc gccggtattg gaaacgaatt gtttttcggt gacgcgtcgc
    34861 accgcctgaa tttggtcgcg aatacgtgcc gcttcttcaa actccagatt ctgactggca
    34921 gtttccatac ggctaatgag ttgcgtaagc acctgatcat ctttgccaga caaaaacagg
    34981 cgcacatact cgacctgctg agcgtattct tcttcactca ccagtccttc aacgcacggt
    35041 cccagacagc gccctatctg atattgcaga cacggacgcg agcgattgcg ataaacacta
    35101 ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg ccagagtttc acgtacggca
    35161 tagccattcg ggaacgggcc gaaatattca cctttggcat gcttcgcacc acgatgcatc
    35221 gccagacgcg ggtgggtatc gccactcagg aagataaaag gataagattt atcatcgcgt
    35281 agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt tgtgttccag caacagcgct
    35341 tcggtttctg tatgagtaac cgttacatca atttgctgaa tctgggcgac cagcgcttcg
    35401 gttttgcgcg aagcgaggtt gctacggaaa tagctggaaa gccgtttttt caggtctttc
    35461 gctttgccga catagataac cgtaccacca gcatcgtaca tgcgataaac gcctggctgg
    35521 ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat cactcactga cttgataatg
    35581 tctccgcatt acacagacca tggcgaattg ccaggtgagt cagctcaaca tcgccatgaa
    35641 tgtttagttt actgaacata cgatagcggt agctgttcac cgttttcgga ctgagattga
    35701 gctgttctga gatctcattg accttctggc ccttggtgat catcagcata atctgcaatt
    35761 cacgttcaga caaactggca aatgggcttt ctgttttttc tggttcgatc tggcttaacg
    35821 ccatttgttg agcgatgtca gaagcaatgt aacgctgccc tgaatagaca gaacgaatcg
    35881 cactcacgac ttcctgcgga gccgcgcctt tgctgaggta gcccgcagca ccggcctgca
    35941 tgactttagc tggtaaaggg ttttctgtat ggacggtaag catgatgatt ttgacatcag
    36001 ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc aatgcccggc atactcatgt
    36061 ccattagcac cacgtcaacg gcatttgtcc ggcaccactt aacggcgtct tcaccgcacg
    36121 atgcctcacc gacgacttta atacccttta tatcttccag aatgcgtcgt atccctgcgc
    36181 gcaccagttc gtggtcatca acaagtagaa cgttgatcaa aggaatatct ccagaaatag
    36241 ggataacgct actgatagtt agtcattcgt atattagcgg tttttattgc aactttgaaa
    36301 cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg tcaattcgat tgtccacagt
    36361 tatggtcacg cccgtcacca acggcttacc ggatcgtttc acagtaagcc ctgcgacgaa
    36421 atgtgacaaa aattaccttt attcagcaaa aatgaaaatc agcccgctga atatgtaaca
    36481 ttaattaacc cgaacaatac gggcaaaaaa cattcatgat aaaaatattt attggtcatt
    36541 atattaacgt attttatagc actgccgata tcacgctcaa aaaacaacca ctgctatttt
    36601 tagcaaagct tatggtatac tccgccgcct taacattttt caccgcaaat tttcattgca
    36661 acatgacgag gaaaataaat gagtacgcct gatttttcta ctgccgagaa taatcaagaa
    36721 ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc tgatgctgca ggcgatggga
    36781 caagctgacg cgggccgcgt gatgttaaag atggaaaaac agcttgcgct gatcgaagac
    36841 gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag
    36901 taatataaaa ccggctgaga gcgtgccttt cagccggttt ttgcatctgg cacgcagaac
    36961 agaaaagaga tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg
    37021 gtgcattgat ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg
    37081 acaaaatcat cgctatctct gctgatgtct tcccttccgc cgtccatttc aaaatttctt
    37141 tttcgcgctt gctgaaattc atctctggcg tcatcactat ttcatcattt aatcgcatca
    37201 gagccatcag actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa
    37261 tgggtatttc gcgcgcgctg caacgggaaa aggacaaaaa gcccagcgcc cggtttggca
    37321 gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc gcgcgcggct tcccataacg
    37381 gctgtgcttc gttgaataag tcatcattcc acattagatg gccctgacta aagttttcag
    37441 ggttcagcac cggatcaatt gcgagaaagt tttttgcctg ataataacta acccacgcct
    37501 cagggtaatt ggtgtagaaa gccactttag gtcgagtgaa tggtaccggg tggcggacac
    37561 ataacgaata gtaatcgtac tccagctgct gagcctgaag ctcaatttca tggtagacct
    37621 cttctgcggt ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat
    37681 ccgtatcctg catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat
    37741 tcaaactatc acataaataa gatttatata taatttatat tattcaggca atgaattact
    37801 tttgcaagcc atagcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt
    37861 ttatcaggct tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct
    37921 tattattgca gcagaaactt ctcgaggaac tggcgggtgc gaggctgctg gggatcagca
    37981 aataacgctt ttgctgcccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg
    38041 tccgcaacat cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc
    38101 tgcgccagct ggcggattgt gttcaggact tcgcccacca gctctggatc cagcgctgac
    38161 gttggctcgt caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgctaca
    38221 cgctgctgtt gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca
    38281 actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg
    38341 atcaccggcc cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc
    38401 tggaagacaa acccgacgtg ctgacgcaac tggcgaatca gcgatttttg ctgacttaat
    38461 gaacatgcag tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc
    38521 agcagattta tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc
    38581 accacttcgc caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat
    38641 tttttcacca agttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa
    38701 gttgattctc aaaatggttc tgcaacgtcg ataacacagt cgccatgatc cagtagatga
    38761 gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct
    38821 gacggaacag ctccggcacc tggattgtcg cggccagcga cgtatctttt accagactga
    38881 taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac
    38941 gcatggtctg ccacggcgtc ataccaatac tggcagcggc ttcccactga cctttatcaa
    39001 tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc ggcagtgttc agcgacagac
    39061 caatcattgc tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga
    39121 tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat agagatataa aaacgcgcca
    39181 gccagcgcac gggccagatt ggcgacaggc gcatcagcgc gagaataaat ccgagtagta
    39241 agccaaaaaa catgccgccg atactgagtt gcagagtata cccagcccct ttcaacagga
    39301 acggcaagga atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac
    39361 gtggggatga taggcgaaca gcgcaggcgc gccgccggta tgaataaaca gaatcggccc
    39421 ttcatctttg aagcgtttct gactgatacc gtcaatcagc cccgccatcg cttttccggt
    39481 atacacagga tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc
    39541 ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca tcccagagta taatttccgc
    39601 tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa
    39661 ttgatcggca acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg
    39721 ttccagccca acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga
    39781 cgaaatatta accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc
    39841 cagcgcgcca agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc
    39901 ttcgactcgc gtcgccagct cttccagttg ggcattggga tcggtcagtg cgtcgcacat
    39961 ttcaatctgg gtattgaaca gatccagcaa caaacgattg ccgttggtta aatagttttc
    40021 cgcggtcgta ccaataggat tttccagcag cgccacgcag tgcagaccga gtttcgcagc
    40081 gactgcggca gtctggcgca catggttaga ctggatcgcc ccggcagtaa tcagcgtatc
    40141 ggcaccttca cgcagagcat ctgccgcgag aaattccagc ttacgtaatt tattgccgcc
    40201 cattgccatg ggggtgacgt catcccgttt gatgaaaatt tcccgtccaa gataatcaga
    40261 aaagcgcggc agatattcga gcggcgttgg cgcgccgata aactccagcc gtggaaaacg
    40321 ggttaaatta tgcagtggca taacagcctc cgatgtgtgt tgttgtgatt ttcttattat
    40381 gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa gcgccctttt ttgtcattat
    40441 gctgattatt tggtcacatc agcaccaaac catttttcgg aaagagcttg cagagtgccg
    40501 tctttttgca tttccgcaat tgcatcattc accgctttca gcaggtcttc atttccttta
    40561 cgcagcgcca cgccagactc ctgacgggag aatgcttcac cggttactgc cagcgtatcg
    40621 ttggttttct tcaccagatc cagcgccgcc agacgatcaa cgaggatcgc atcgatacgg
    40681 cctacgcgca gatcctgata tttggtcggg tcatcatcat aggtacgcac atcgacgccc
    40741 tgaacattct gacgcagcca ctcttcatag ttggtgccca gaccgacacc cacttttttg
    40801 cctttcagat catcagccgt tttaatggtg ccttcgttgc cttttttcac cagcgcctga
    40861 ataccagaaa tggtgtacgg ggtagagaaa tcgtattttt tcttgcgctc gtcagaaatg
    40921 gtgacctggt taatcaccac atcaatgcgt ttagagtcca gcgacgccag cataccgtcc
    40981 catttggtcg gttttagtga cgcctcaacg ccaagatgtt ttgccagctg ttgggcaaat
    41041 tccacttcaa aaccggttaa tttgccgtca tccccctgaa aactgaacgg cggataagtt
    41101 ccttccagcc ctaccagcag cgtgccgcgc tctttgactt tattaagcag accttcatct
    41161 gcaaaacttt taacgctcat acccgcaacc agcgccacgg ccatcacacc catcaatgcc
    41221 tgacgtccca gatgtgctaa tttcatattt accccgaatg ttgttatgtc tgtttgcagt
    41281 gtagagccat tgtttgtaaa cacaaaaaca actccgctac atcttattct tatttaatat
    41341 atatcagaag caggttggct ggaggatttc tgcacaagtc cggtacacgt ttgacgctga
    41401 tagtgttgat acttccgtaa cgcgatgcgg taattgttgg tgctggtggt gggcagccac
    41461 ggggcgagga tttcatcaag gaaaccgtcc tgcagctgag cgagggaaat attttgctcg
    41521 tgtagatgat tccccaaacg gcgcagacga acgacatatt cacgcaccgt accagggctc
    41581 atttcagttt gctcaaaaag aaattgctta aagccgataa tatcgaaaaa attactctgc
    41641 gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg cccatgattg ttgttccact
    41701 tgccagccat tttcatcaag cagcgtgtcc aggcgggaaa tctcgatttt attcactatt
    41761 ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac ggcaatgcgc gcaatgggtc
    41821 tggctgtgtt taaagtcttt aagatagcgg cttaatggcc gtcttttcag gtgctgcacc
    41881 atcattaaga actcctggta gtcaaaatta aagtgcggca tttactgacg ttataactta
    41941 cccagtttag tgcgtaaccg tttaatagcc tggctgtgta actgactgac ccgcgattcc
    42001 ccgacctcca gcaccgcacc aatctctttg agattcagct cttcctgata atagagggtt
    42061 aataccagtt tttcgcgctc cggcaacgtt tcgatggctt ccatcacccg ctggcgcaga
    42121 ttactgtcca gcagttgttg tagcgggttt tctcgctgat gatcatcagt aaccagttcg
    42181 atgctatcgc cgtgctcttc gcgccactca tcgtaggaga agagctggct gttattggtg
    42241 tcgagcaaca tttggcgata atcggcaata tcgatcccta aacgttccgc aacctcagtt
    42301 tccgtggcgt tgcggccaag ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc
    42361 gcgttgcgac gcacgctgcg cggcacccag tcacggctgc gaagttcatc cagcatagcg
    42421 ccacgaatac gctgcactgc gtaagttgta aatgccgttc cttgtagagc gtcatagcgt
    42481 tcgacggcat taagtaaccc aatgccgccc gcctgtagca gatcgtcaag ttccacgctc
    42541 gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc
    42601 cacagcgagt gtttatccat tacaccttca gcggtataga gtgaattcac gataaacagc
    42661 cctgcgttat atgagttatc ggcatgatta tccgtttctg cagggttttt aatcggacga
    42721 ttagtgggtg aaatgagggg ttatttgggg gttacggcta tttgccgggt agaaaaaacc
    42781 ccgccggagc ggggtttggc agaatatagc ggcgattaac gcagcagaga cagtacgttc
    42841 tgcgtggtct ggttagcctg tgccagaaca gaagtacctg cttgttgcag gatctgcgca
    42901 cgagacatgt tagacacttc ggtcgcgtag tcagcatctt cgatacggct acgggcagaa
    42961 gacaggttgt ttacggtgtt gccaaggttg gtgatggcag agtcgaaacg gttttgaatt
    43021 gcacctaggt tagaacgcaa cgagtcaacc tgagacagtg ctttatcgat agcagccaaa
    43081 ggatcttctg ttttctgcgt agaaattgct gcaatagaat cagttttcag gctcttacct
    43141 tgtaaatcac taacactgta agttttgcca tcggttgcag ttacaacttc ggttttgcca
    43201 tcgcttccac ccagttcaac tttaacattg tcgtgtttga caccgtcagc atcagtgtaa
    43261 gacattgttt taacggaaac tgcacctgtt ttctgatcaa cattcgctgc atactgttta
    43321 ccgtcatcac caatcaacgc aaatgaacca gtatctttgc cattagcgtc ttctaactta
    43381 aagagttttg cacctgtgat agaagcggtt gcggtatcag tacccaactg aacatcaata
    43441 gcccccttcc ctttcaccgt tgtgacttcc tttaaattac ctgcttgagg ctgggtaccc
    43501 gctagagccc ccagcttcag cttacctgca tcaggagatg tcggatcatc ctccacagta
    43561 acttcataat aaccattttc aggggattta tctgaaccgg caactttaac gtacaactta
    43621 ccgttagcat cagacataat attaccgtca cgtttgcccg gagtcgtagt gcctttaact
    43681 gtagctgtaa tatctgcatc aagtgtatta gcgtcagcaa gcttaactgt cgatccaaaa
    43741 taggttgttt cagctgttgc ggcttttaat ttagtaggat ctttaatacc aaaaccagat
    43801 aatcccagtg tgtcggaatt aatttcctgc agtttaatag taattgtttc gccgtcgttt
    43861 gcaccaacct gaatagtcat atcctgatca gaagccagca ctttaacgcc gttaaactga
    43921 gtctgaccag aaacacggtc aatttcactc agacgttgct ggatttcgga ctggatggag
    43981 gtcaggtcac tgtcagagtt agtaccgtta gttgcctgaa cagaaagttc acgaatacgc
    44041 tgcaggttgt tgttaatttc attcagcgca ccttcagtgg tctgcgcaac agaaatacca
    44101 tcattcgcgt tacgggaagc ctgagtcaga cctttaatat ttgccgtaaa acggttagca
    44161 atcgcctgac ctgctgcgtc atctttagca ctgttaatac gcaggctaga agagagacgt
    44221 tcaatggcgg agctcagaga agactgagat ttattcaggt tattctgggt caacagcgac
    44281 aggctgtttg tattaatgac ttgtgccatg atcttttcct tatcaattac aacttgatgt
    44341 tattgggctg ttgcccacgg tttctcaccg taccccttgt atcggcacct gaatttcgaa
    44401 ctttagaaaa ttttagtttc tctcctgttt tttcttaacg gctgaaatat ccgtaatata
    44461 caactctact ctgcgcatca tcttcgtgaa tcaactatta aactttgcgc acaaattgcc
    44521 gataacccgc ttatctactg tttgcaatca aaaggaatta ggtatggctt catttacatc
    44581 tcttggcgtt ggttcgaatc tgccgctgga tacattgcta aacaacctga ccatcgctga
    44641 aaaaaaacgc ttaaacccaa ttactcagca acagagtgac aataccgcac gtctgacagc
    44701 ctatggcaca ttaaaaagtg cattagaaaa attccagact gcaaatactg cgttaaataa
    44761 agccgatctg ttcaaaagca ctaccgttac cagtagcaat gaagacctta aagtttccac
    44821 cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg cgcaggcgca
    44881 atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac tgggtgatga
    44941 gtcatcagat acacgcacaa taaaaattac ccaagacggc cgtaaagagc cgctggaaat
    45001 caagctcaat aaagatcaga cgtcattggg tgagatcagc aaagccatta acgatgccga
    45061 tagtggaatt tccgctagta tcgttaaagt caaagatggt gactatcaat tggtattgac
    45121 cgccagtgaa gggctggcca ataaaatgac catatctgtt gagggtgaca gtaaattaaa
    45181 tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac tggttaacgc
    45241 gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta acaaaattac
    45301 cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg tccgggtcac
    45361 ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg attcatataa
    45421 ctccctcatt gacaccttta acaccctgac aaaatataaa gaagtagacc caggtgctga
    45481 agcacaagat aaaaataatg gtgcacttct cggagacagc gttgtacgta caattcaaag
    45541 cggcatccgc gcacaatttg caaacggtgc cagcgacggt gccttcaaaa cattaaacga
    45601 gatcgggatt aagcaggacg gcacgacggg taagctgaaa attgatgatg ataaactcaa
    45661 aaaagtcctg aatgaaaata cagcatccgt acgtgaattg ctggtaggcg atggtaaaga
    45721 aacaggaatt accaccaaaa ttaccaccga ggtaaaaggt taccttgccg atgatggcat
    45781 tattgatagc gcacaggaca gtattaacgc cacactgaaa aaactgacca aacagtatct
    45841 atccgtcagt gccagcattg atgacaccgt ggcgcgttac actgcacagt ttacgcaatt
    45901 agataccatg atgagcaaac tgaataacac cagtacctat ctcagccaac aatttactgc
    45961 aatgagtaat tcctgatcac ggaggtcagc atgtataccg cgagcggctc aaaagcctat
    46021 gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct ggtcaccatg
    46081 ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca ggacaacaat
    46141 cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg
    46201 gtgagtcttg atgaagatag caaagacgaa ttaacccaaa acttgattgc tctttatagc
    46261 tatatggtca ggcgcttgct gcaagccaat ttacgtaacg atgtctccgc agtcgaagaa
    46321 gtggaagcat taatgcgtaa tattgccgat gcctggaaag agtcgttact ctccccttct
    46381 ttgattcagg acccagtctg atgaaccatg caccgcattt atatttcgcc tggcaacaac
    46441 tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca
    46501 tcgccagcga aatggcgtat gttaatgcgg tgcaggagat tgcacatttg actgaagagg
    46561 ttgacccgtc caccacgatg caggagcagc tccgcccgat gctgcgcctg attctcgaca
    46621 acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg aaactggtcg
    46681 gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc
    46741 tggctccgca ggataacctc ttttgaatct gaatgagtcg atggctcgcg aataatccga
    46801 ttacggctac gcttctaatg ttccccttga atggagtcga agaatgcgta atcccacgct
    46861 gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg aattggccga
    46921 gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa
    46981 aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg atttaggtga
    47041 gtttgatcag aaaggcagca tccctactaa atatggcgat aaagtacaac tgctggccgc
    47101 cattgacgcc ctgaaacgta atgacattgc ggtgctgttg gacgtggtag tcaaccacaa
    47161 aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg acgaccgtac
    47221 gcaaattgat gaagaaatca ttgagtgcga aggctggacg cgttacacct tctccgcccg
    47281 tgccgggcaa tactcacagt ttatctggga tttcaaatgt tttagcggta tcgaccatat
    47341 cgaaaaccct gatgaagatg gcatatttaa aattgttaac gactacaccg gcgaaggctg
    47401 gaacgatcag gttgatgatg aattaggtaa tttcgattat ctgatgggtg agaatatcga
    47461 tttccgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg tgatggaaca
    47521 aacgcaatgc gacggttttc gtcttgatgc ggtcaaacat attccagcct ggttttataa
    47581 agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg tggcggagca
    47641 ctggtcgcat gaagttgata agctgcaaac gtatattgat caggtggaag gcaaaaccat
    47701 gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg gacgcgacta
    47761 cgacatgacg cagattttca ctgggacgct ggtggaagcc gatccttttc acgccgtaac
    47821 cctcgttgcc aaccatgaca cccaaccgtt gcaagccctc gaagcgccag tcgaaccgtg
    47881 gtttaaaccg ctggcgtacg ccctaatttt gttgcgggaa aatggcgttc cttcggtatt
    47941 ctatccggac ctctacggtg cgcattacga agatgtcggt ggtgacgggc aaacctatcc
    48001 gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc
    48061 ccacggtgta cagacgttat ttttcgacca tccgaactgc attgccttta gccgcagtgg
    48121 caccgacgaa tatcccggct gcgtggtggt catgtcgaat ggggatgatg gcgaaaaaac
    48181 cattcatctg ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca
    48241 agagagcgta gtgaccgacg aaaacggcga agcaaccttc ttttgcaacg gcggtagcgt
    48301 cagcgtgtgg gttatcgaag aggtgattta aatttatccc cggcggcaag ccggggagat
    48361 ttcattacgg caatggcgtc ggtaacggcg ctttatccag cgcggcggca cactctgccg
    48421 tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct
    48481 caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac atcaatgtca
    48541 acttacccgg cacggcaatc acccgctgcc actgacggca atccagggta tcgccctctt
    48601 tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc ggccctttgg
    48661 tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt
    48721 cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt t
//
LOCUS       DAZFZC010000027        48319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFZC010000027 DAZFZC010000000
VERSION     DAZFZC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.027"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..712
                     /locus_tag="ACHKXP_001786"
     CDS             <1..712
                     /locus_tag="ACHKXP_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646811.1"
                     /translation="AFDDDIDFYLKNGYLSTESNIRGIIEEKNKQYKEHKALCEVNNV
                     WDIFSDSFKDNESEFISKIKCVINDNLSHIPVAHFIGLIDILNRLEIDCDSYIEAYAD
                     AFVSQDDAYTAFRNLYVEIFGNEKLGLLIQKKLMDKKPEETNLENVLYKIIEGKFNHS
                     DIAYLNSFSEDDYLKWILSRNQDALDLVRKGLLRFKSMQELTEEQQKITNKAIGALTK
                     LASRSSLNKLRVSRLLNN"
     gene            891..1631
                     /locus_tag="ACHKXP_001787"
     CDS             891..1631
                     /locus_tag="ACHKXP_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646812.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILERLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            1675..3015
                     /locus_tag="ACHKXP_001788"
     CDS             1675..3015
                     /locus_tag="ACHKXP_001788"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO7646813.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFPDVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            3163..4278
                     /locus_tag="ACHKXP_001789"
     CDS             3163..4278
                     /locus_tag="ACHKXP_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO7646814.1"
                     /translation="MSELKELITELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGYNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKIACLGEEGL
                     DPGDATPGQIIKAYYCAKPYSRRLELLSKVKGIAALV"
     gene            complement(4270..5415)
                     /locus_tag="ACHKXP_001790"
     CDS             complement(4270..5415)
                     /locus_tag="ACHKXP_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7646815.1"
                     /translation="MKYIKSSSLLALTLLFSSGFVNADNKQTLIEAATAGDTAAQSEL
                     GTNYFDGINGFDKDVVEAKKWIDLAAEKGDKVAYYALGVMYTFGEGVDKDLNKAVEYY
                     KLAGDAREGRAYNNLGAIYQKGMLGKVDHALAIKYFNLASDAGYVKASYLLGAYYQYG
                     KGVQKSYKKAFTYYKKAADQGSSDGMFALGTLYDDGLGVKRNDAEAIKWYKKAAELGN
                     ADAMTNLGIMYEYGEGVKKDYKKAAELYQAACDKEDKNGCDYIAELKESGKYRAPASK
                     AKTKSATQRLIAKSIDKGVNATFTWQGDDATFTANDGKVDCTFLKDFSEKGGNLATSF
                     VCTDNVQIILKQFRDTKNAYLAVMTDNFNTEVKSFSVNVYVTNTGSN"
     gene            5649..6062
                     /locus_tag="ACHKXP_001791"
     CDS             5649..6062
                     /locus_tag="ACHKXP_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646816.1"
                     /translation="MSKNKLILTGALLSLFALVGCKPTDEKAIALGQQAIADDMKDPT
                     SVMFRKDKFVRTDHDDGSVTGFVCGELNAKNGFGAYVGYHSYVVELEMKPKGMFSKGV
                     VYKILSKSLAPNDPHDLQRYKQVYRKMCNSEAVAH"
     gene            6188..6964
                     /locus_tag="ACHKXP_001792"
     CDS             6188..6964
                     /locus_tag="ACHKXP_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646817.1"
                     /translation="MKDEIQKLACDIIDKTGLEISEGSRLDIIGKAVNTAMNHIAARM
                     DEIPLPGLPYLKVELHVWGEPSCARRSALVVFISKENPLSLKVQVGAWLDGRVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLINKGANSAVNVIRESEYASLWDMCKSQLDLVHIVVDAGKACDGVMAEFAGKMVRIA
                     NELPMIKQEAKSYATNHVTELLAPYRLESDQRKMISWGSW"
     gene            7020..7502
                     /locus_tag="ACHKXP_001793"
     CDS             7020..7502
                     /locus_tag="ACHKXP_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646818.1"
                     /translation="MAVIVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTIKRKRDSVTINSMVKKSATSQTAITDVYAALLEETTKETHEQIDQHMEAKQQ
                     "
     gene            7499..8821
                     /locus_tag="ACHKXP_001794"
     CDS             7499..8821
                     /locus_tag="ACHKXP_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFO7646819.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVVEPLSESVPSDEKAIDILNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            8818..9033
                     /locus_tag="ACHKXP_001795"
     CDS             8818..9033
                     /locus_tag="ACHKXP_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646820.1"
                     /translation="MIVLSKREKETLHEISKWPEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKVLLQQLVESGALK"
     gene            9179..9469
                     /locus_tag="ACHKXP_001796"
     CDS             9179..9469
                     /locus_tag="ACHKXP_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFO7646821.1"
                     /translation="MSNLTSWDWWLATYFLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            10248..10322
                     /locus_tag="ACHKXP_001797"
     tRNA            10248..10322
                     /locus_tag="ACHKXP_001797"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10280..10282,aa:Asn,seq:gtt)
     gene            10328..10414
                     /locus_tag="ACHKXP_001798"
     tRNA            10328..10414
                     /locus_tag="ACHKXP_001798"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10362..10364,aa:Tyr,seq:gta)
     gene            10419..10494
                     /locus_tag="ACHKXP_001799"
     tRNA            10419..10494
                     /locus_tag="ACHKXP_001799"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10452..10454,aa:Thr,seq:tgt)
     gene            10685..10771
                     /locus_tag="ACHKXP_001800"
     tRNA            10685..10771
                     /locus_tag="ACHKXP_001800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10717..10719,aa:Ser,seq:tga)
     gene            10993..11343
                     /locus_tag="ACHKXP_001801"
     CDS             10993..11343
                     /locus_tag="ACHKXP_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO7646822.1"
                     /translation="MTSKENTPLKVTPEHIEQLIQSEHYFTAYDASHGDNFISVYNSK
                     TDIDKGHESLKLLTFCVMVLKNGYTVTGKSACVRPEIFNFDVGREYARKDAIDQIWPL
                     EGYLLKQKWHEAKQ"
     gene            11340..11585
                     /locus_tag="ACHKXP_001802"
     CDS             11340..11585
                     /locus_tag="ACHKXP_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HFO7646823.1"
                     /translation="MTITIYGRDNCSYCKRAVELAKQLKGHGYGDYEYIDITTAGIDK
                     EKLSEIVGKPVETIPKVLIDGQPIGGYTELAAYVSTL"
     gene            11796..12899
                     /locus_tag="ACHKXP_001803"
     CDS             11796..12899
                     /locus_tag="ACHKXP_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7646824.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAVMISQVSNDMRFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSSLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELE
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRVNFS"
     gene            complement(12894..13280)
                     /locus_tag="ACHKXP_001804"
     CDS             complement(12894..13280)
                     /locus_tag="ACHKXP_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7646825.1"
                     /translation="MSDNKIEFIESRYAAFIAGLIESSPMSQAQIAKTIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            13532..13744
                     /locus_tag="ACHKXP_001805"
     CDS             13532..13744
                     /locus_tag="ACHKXP_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646826.1"
                     /translation="MNTTISSLIALEIGHVQKLADECVADILTDLPNEQIQVGVNDTT
                     GFIFELNNKRFTLLNTGSGSLAVRIC"
     gene            13843..15951
                     /locus_tag="ACHKXP_001806"
     CDS             13843..15951
                     /locus_tag="ACHKXP_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFO7646827.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVCYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSWSLW
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDAEQTDASGENNEDNEDHETPSALDNHKSIKSES
                     HSKHKHDNNDGDDSDNSESSETIFDDTENDKEVSDSDASDNAVSESFTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASAYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVNSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            16047..17282
                     /locus_tag="ACHKXP_001807"
     CDS             16047..17282
                     /locus_tag="ACHKXP_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7646828.1"
                     /translation="MTTTALQNEKNHSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKEAEINMLINA
                     GIVGEYAEKMVKFAGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            17463..20981
                     /gene="dnaE"
                     /locus_tag="ACHKXP_001808"
     CDS             17463..20981
                     /gene="dnaE"
                     /locus_tag="ACHKXP_001808"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO7646829.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRAKLAKESKGCMESLERGRNYSFTALIK
                     NEKGYRDICELMTAANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELRNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDAISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTLKTVEEALEFGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNPKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKS"
     gene            21107..21538
                     /locus_tag="ACHKXP_001809"
     CDS             21107..21538
                     /locus_tag="ACHKXP_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646830.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDDARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            21656..22684
                     /locus_tag="ACHKXP_001810"
     CDS             21656..22684
                     /locus_tag="ACHKXP_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO7646831.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVATASATEDEGLDD
                     LLRELDSL"
     gene            22745..23689
                     /locus_tag="ACHKXP_001811"
     CDS             22745..23689
                     /locus_tag="ACHKXP_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFO7646832.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVSRMAPQPTVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDNSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVNKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            23689..23955
                     /locus_tag="ACHKXP_001812"
     CDS             23689..23955
                     /locus_tag="ACHKXP_001812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646833.1"
                     /translation="MLKSIINGATTTPAQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            23958..26297
                     /locus_tag="ACHKXP_001813"
     CDS             23958..26297
                     /locus_tag="ACHKXP_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HFO7646834.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKCVTADTMLLTERGMVTVKELFEIEGYKATCTTRDVEHN
                     VGLINENGVIEKTSHLTWNNRRKFKRIKLASGGYIEATFRHPIRVVDDLGNVVWRHAE
                     KISVGDTIPSMVGTHQFGDQHLDANIAKLMGYLIADGYVASENSVNFSNTDPFIKDEY
                     YRLISLVSDKMPVTRKHNGSEDHVLFSKEVRSLLFKEYGLEYEKAAGKQVPLSVRRAN
                     SEAQIAFLRGYFELECHVNDGRCIEVVSASGLLLQQIRLMLLNLGITSTISEKHVAGY
                     ENTYYRLSFSGSNYDLFLSTIGFESPARLSVATKRDIGFDRTYSGYVPHISGLVKSLY
                     ESLTKTTREDYALVDHVIGRGDRVGIDKLREIYVSFIGRKNRFNEHLFAQLAAVIGSN
                     LFYDEVVAIEEGEAPTFDVAMPETHSFWSNGIISHNTFLATAAMISAQKQDGLAVFLD
                     HENSFDVGLAVANGLNADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPI
                     CIVADSLASMVPNSKAEKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARK
                     YNACIIFLNQVRTKIGVMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVG
                     AECIKNKVAPPYGKCTWKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKY
                     TKSQIVEMYREKPLAEIIAALQAIDDRRAKDNPTESVEE"
     gene            26390..26590
                     /locus_tag="ACHKXP_001814"
     CDS             26390..26590
                     /locus_tag="ACHKXP_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646835.1"
                     /translation="MIKIYLLAVATGLSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            26594..27424
                     /locus_tag="ACHKXP_001815"
     CDS             26594..27424
                     /locus_tag="ACHKXP_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7646836.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            27516..27941
                     /locus_tag="ACHKXP_001816"
     CDS             27516..27941
                     /locus_tag="ACHKXP_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646837.1"
                     /translation="MTAIKKLYDAANVALDVIDDEAAKGFPEPDWAHQLRNAIAEMNP
                     PDPTTDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            27997..28272
                     /locus_tag="ACHKXP_001817"
     CDS             27997..28272
                     /locus_tag="ACHKXP_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646838.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKADGLNDEETDALIQRILVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISDEDAAHLERILSISTFE"
     gene            28488..28823
                     /locus_tag="ACHKXP_001818"
     CDS             28488..28823
                     /locus_tag="ACHKXP_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646839.1"
                     /translation="MKAIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            28823..29035
                     /locus_tag="ACHKXP_001819"
     CDS             28823..29035
                     /locus_tag="ACHKXP_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646840.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(29615..30829)
                     /locus_tag="ACHKXP_001820"
     CDS             complement(29615..30829)
                     /locus_tag="ACHKXP_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO7646841.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNLDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKLDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            31031..31675
                     /locus_tag="ACHKXP_001821"
     CDS             31031..31675
                     /locus_tag="ACHKXP_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646842.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            31930..33015
                     /locus_tag="ACHKXP_001822"
     CDS             31930..33015
                     /locus_tag="ACHKXP_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFO7646843.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            33245..35161
                     /locus_tag="ACHKXP_001823"
     CDS             33245..35161
                     /locus_tag="ACHKXP_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7646844.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERFKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHENNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            35151..35894
                     /locus_tag="ACHKXP_001824"
     CDS             35151..35894
                     /locus_tag="ACHKXP_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646845.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NYPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            35904..36473
                     /locus_tag="ACHKXP_001825"
     CDS             35904..36473
                     /locus_tag="ACHKXP_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646846.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            36547..38862
                     /gene="nrdA"
                     /locus_tag="ACHKXP_001826"
     CDS             36547..38862
                     /gene="nrdA"
                     /locus_tag="ACHKXP_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7646847.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            38963..40105
                     /gene="nrdB"
                     /locus_tag="ACHKXP_001827"
     CDS             38963..40105
                     /gene="nrdB"
                     /locus_tag="ACHKXP_001827"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7646848.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            40183..41052
                     /locus_tag="ACHKXP_001828"
     CDS             40183..41052
                     /locus_tag="ACHKXP_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFO7646849.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            41224..42327
                     /locus_tag="ACHKXP_001829"
     CDS             41224..42327
                     /locus_tag="ACHKXP_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO7646850.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIDTFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTEAELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDEILILGYDPLPWIKVPVAV"
     gene            42329..42742
                     /locus_tag="ACHKXP_001830"
     CDS             42329..42742
                     /locus_tag="ACHKXP_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646851.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVSDADAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNSTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            42739..43215
                     /locus_tag="ACHKXP_001831"
     CDS             42739..43215
                     /locus_tag="ACHKXP_001831"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFO7646852.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            43215..43859
                     /locus_tag="ACHKXP_001832"
     CDS             43215..43859
                     /locus_tag="ACHKXP_001832"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7646853.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            43921..44340
                     /locus_tag="ACHKXP_001833"
     CDS             43921..44340
                     /locus_tag="ACHKXP_001833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646854.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            44350..44907
                     /locus_tag="ACHKXP_001834"
     CDS             44350..44907
                     /locus_tag="ACHKXP_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFO7646855.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMGACSAWIAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            45068..45877
                     /locus_tag="ACHKXP_001835"
     CDS             45068..45877
                     /locus_tag="ACHKXP_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646856.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVIKAESVESAENEPEQL
                     GFIEVEECVEVNDELKVQQSNDSNTNKIARTARGPRFTLSDKDDLFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            46060..46653
                     /locus_tag="ACHKXP_001836"
     CDS             46060..46653
                     /locus_tag="ACHKXP_001836"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7646857.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPIFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            46838..47068
                     /locus_tag="ACHKXP_001837"
     CDS             46838..47068
                     /locus_tag="ACHKXP_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646858.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            47598..48242
                     /gene="rnhA"
                     /locus_tag="ACHKXP_001838"
     CDS             47598..48242
                     /gene="rnhA"
                     /locus_tag="ACHKXP_001838"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO7646859.1"
                     /translation="MCARARKKIGAALDSGVYMTTKTPARSQAKTRKKAKNKNSPRTN
                     PTTPVVEFNPQLKTVKIFSDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRM
                     EMMGALIGLERLKYPCNVILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWK
                     RLDEAASRHNVRWKWVKGHAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNK"
BASE COUNT        13723 a        10506 c        11564 g        12526 t
ORIGIN      
        1 tgctttcgat gatgacatag atttttatct caagaatggt tatctttcta ctgaaagcaa
       61 tattcgaggt ataatagaag aaaaaaataa acaatataaa gaacacaagg ctttatgtga
      121 ggttaacaat gtttgggata tttttagtga ctcattcaag gacaatgaat ctgagtttat
      181 ttctaagata aaatgtgtaa ttaacgacaa tctttcacac atacctgttg ctcacttcat
      241 aggcttgatt gatatcttaa atagattgga gattgattgt gatagttata tagaggcata
      301 tgcagatgcc tttgttagcc aagatgatgc ttatactgct tttcggaatt tgtatgtaga
      361 gatatttggt aacgaaaagt taggtctgct aattcaaaaa aaattgatgg ataagaagcc
      421 tgaagagact aatttagaga atgttttata caaaataatt gaaggcaaat ttaatcatag
      481 tgacatagca tatcttaaca gtttctctga agatgattat ttaaagtgga tattatcccg
      541 caatcaggat gctttggatt tagtgagaaa ggggttgctc agattcaaaa gtatgcaaga
      601 actaacagaa gaacagcaaa aaatcacgaa taaagcaata ggtgcattaa cgaaactggc
      661 ctctagatca agtcttaaca aactacgtgt gagcagattg cttaataatt aagtattcat
      721 gacctgtttc tgtatataaa taataagtaa cttattaaat atacggaagc aggtctttca
      781 aaagataccc accagaacaa ctcccttccg tttccacttc caaaaactgc caccaatcgc
      841 tatcatcagc tcattgtgat aagtaagtaa ctacctacca ggtgagccac atgagccaaa
      901 tctttttcga caccatcgac aacgaccagt acgacttcat gacagagtgg aataccgctg
      961 ttatggataa gtgggttgct gaaaacattg gtttgtcgcg ctgcaaagac gaggctgaac
     1021 tcttcgagac gaagtggttt gattaccgcg acatgcatcc tctcatggcc acctgccttt
     1081 ttacggaggc atacaaacgt cagtactcaa atatcatgct gacgcacggt cgcgaacact
     1141 ttgaaacagc tccgttcacc accgggttaa aacgcctgcc ttatcaggag ttgtcgactg
     1201 ccaataaaac gtctctatgg aaagcacgcc agtttgctga ccgctattgc tgctcatacg
     1261 actactttat ctccaccgtt ctttccgcag ctgcacgacg gctgtgggac aagctgccgc
     1321 gcccacagca tttatggcag ccagaactga ttgagatatt tgaagagaaa ttagccagac
     1381 gcgcaacaac ccgtctggat gactctctgg ttagctttaa gcatatggga gacatgcagt
     1441 tcaacccgat tcaggaaagc tattttgaat ggattctgga gcgtttgcgc accatccctc
     1501 gcagtaagcg catacgcgca attttctccg ctatctggct aatggaaatc gttccagagc
     1561 gccttatttc cgcccacttt ccagaagaac tggaagaagc acggcggttt attgatcccc
     1621 tatctaatta actaatacta gaaaacaatt tgtttaaaaa acaaaggaaa gcacatgacc
     1681 gaactttgcc atacaggacg cgggctgtcc gaagagtttg atgaagattt ccagaacaga
     1741 ctgacggcat atttttgtcg tgatcacgag tttcttactc gtgcgggaga tctggttgtg
     1801 cctagccaat ttgccaatgc ggccaatgcc atattggtta atatggtttc gggctattac
     1861 cgtatgtaca agagcgcgcc ttcttcatct gcgattctgg atatgcttaa gcgtgcgaaa
     1921 cgcgataaga ccatccgtga ggaactcttc ccggatgttg ttgctgcgtt taagcgcatc
     1981 cttgcagaaa aattgtctga tacctcgtac atggttgacc aggtatccac attcgcaaaa
     2041 agtgtagcgt ttgatgatgc tctgattaaa gctgctgaac tgaaagagaa gggcgacttt
     2101 cagggggcga tggcaatcat ggccaaggtt cagcagatag gctcgaacga agcgaccgga
     2161 atctatgact actacacctc cgcaagtgag cgattgaaag cgcgtgaata tgaggcttca
     2221 gaggagtatg tgccaaacag cattacaact ggactccctc tgcttgatag attgctgtac
     2281 caaaaaggct gggcaaagcg cgaaatggtg ctcttcatgg gttttgctaa atccggtaaa
     2341 tcgaccgcaa tgggtgagtt ttccataaac gcaacgcttg ctggctacaa tgttctgtat
     2401 ctctcgctgg aggttcacac caccatttta tccgaccgtt ttgatgcaag attgtcggag
     2461 acagaaatgt ccaagctggt ggaacggcgc gatgaggttc atcgtaagtt ggcagagttg
     2521 ggagccacga aggggattgg tagtttgtgg gtggttgagc gtccgtcagg aagtatgtca
     2581 ccggcagatc tggaccgtat gcttaacagc atgaaagcca acggcatggt gcctgacatg
     2641 gttgttgtcg actacgcaga tttgatgcgt gccagttatg accttcgtga tgatcgcgcc
     2701 aacattcgta gtatctacac cgatttacgt gctctttatg acaagcataa cgttgctgga
     2761 ataacggcat cgcagacaaa ccgtgaaggt ggcgcgtcag aagttgccac aatgatgcac
     2821 gccgccgaca acatcgaaaa agtacgtatt gctgacctgg taataacgat caacaaaacc
     2881 gaagaagaag aagcgaaagg agaggctcgt ctctactttg ctggttcgcg taaccagcag
     2941 ggagggatca gcattcgcgt taaacaaaac ctcgaacaaa tgcgcttcat tgagcgaatc
     3001 ttagacgtta cctaaaaaat aagcgtggag aacacctcca cgcttgattc attggtgaaa
     3061 caacttttct tttgccaaac cacaaaagaa aaacacatga gcctttatgt tatatcaaca
     3121 tttaggttgg taacaatatt gcctgttaaa agtggaatta tcgtgagcga gctgaaagag
     3181 ttaattaccg aattagattt tgaacaatgg ttggacactg aaggtatcgt ttatcgacgt
     3241 ggaggcgtga gtactcgcgg tcgtgaagtg aatatcaagg agtgtccggt atgcggcagc
     3301 tccaactgga aggtatattt caatctgacc agtggcgtcg gtaaatgctt cgctggtgat
     3361 catcccgaag agattcagtt caataagctg gtcttcctca agcactacag cggcaaatca
     3421 cgacgacagt tcgaggaata tgtgcagaac gcccttcttt cccaggggtg ggcaccaaag
     3481 aaagaagagc tagtgcttgc aagcacagtc gagttagagg ggccagttgc actccctcgt
     3541 cattacgaac ttcctataga tggccgtctt ccagactatc tggttgaacg aaatatatcg
     3601 cctgaaatgg caaagtattt tgacctacga tactgcgtcg aaggtaagca cgcctatgta
     3661 gatccgtata cagatcaggt taaaggacag atattcgata tgcgaatact gataccggtt
     3721 tacgatctgg atggggtaat gaagacattt cagggacgag acattaccgg tacagcagaa
     3781 cgccgctacc tctttcctat gcagcttcca gcttcaggta agtttctcta caacggctat
     3841 aatgcagtcg gcaaacagac tgtagttgtc tgtgaggggg cgttcgatgt tatgggggtt
     3901 aaacgagcta tttttgacga agaaacatta cgtgattacg tggaaccaat aggaacgttc
     3961 gggatgcatc tatctggtaa caccactcag gatgcagaag atcagttagg cgcgttcctg
     4021 acgctcaagg cgcgtgggtt acgtaatgtg atcatgatgt gggatagtga aaagcaagct
     4081 atacgaaaca ctatggccgc agccaggcga ctgaccagta tcggtcttaa tgtgaaaatt
     4141 gcatgtttgg gcgaggaagg actcgacccc ggcgatgcga ccccggggca gattatcaaa
     4201 gcctactatt gcgcaaaacc ttactcacga cggcttgaac ttttaagcaa ggtaaaaggc
     4261 atagcagcat tagtttgaac cagtattagt cacgtacaca ttgaccgaaa atgatttaac
     4321 ttctgtgttg aagttgtcag tcattaccgc gaggtaggca ttcttggtat ctctgaattg
     4381 cttcagtatg atttgaacgt tatcggtgca aacaaacgag gtagccagat tgcctccttt
     4441 ctctgaaaaa tccttcagga aggtgcagtc aactttgcca tcattagcgg tgaaggtagc
     4501 atcatcaccc tgccaggtaa atgtagcgtt aacgccctta tcaatggatt tagcaataag
     4561 tcgctgggtg gcggattttg ttttggcttt ggaagccggc gctcgatatt tgccgctttc
     4621 tttcagttct gcaatgtaat cacagccatt tttatcttct ttatcacaag cagcctgata
     4681 aagctcagct gcttttttat agtctttttt aaccccttca ccatactcat acataatgcc
     4741 aagatttgtc atggcatcgg cgtttccgag ttcagcagct tttttatacc acttaattgc
     4801 ttcggcatcg ttgcgtttaa cacctaaacc gtcatcgtat aaggtgccta atgcgaacat
     4861 gccatctgaa ctaccttgat ccgccgcttt tttgtaataa gtaaaagctt ttttataact
     4921 cttttgtaca cctttaccat attggtaata cgcaccaaga aggtagctag ctttaacata
     4981 cccggcatca gaggctagat tgaagtattt aatggcgagc gcatgatcta ctttaccaag
     5041 catccccttt tgataaatag caccaagatt gttataggct ctaccttcac gagcatctcc
     5101 cgcaagtttg tagtactcaa ctgctttatt taagtcttta tctacaccct cgccaaatgt
     5161 gtacataacc ccaagtgcat aatatgctac tttgtctccc ttttctgcag cgagatcaat
     5221 ccacttcttg gcctcaacta catccttatc aaaaccattg ataccatcaa aataatttgt
     5281 ccctaactca ctttgtgctg cggtatcccc tgcggtagcg gcttctatca gcgtttgttt
     5341 attgtctgcg ttaacgaatc cagagctaaa aagcaaagtt aatgccagca acgatgaaga
     5401 ttttatgtat ttcatatttt ttgttctgtt gatataggat gaacagttta gacgcggcaa
     5461 gtgtcaagtg tgcgaaaaat ggtacttaaa tttagttaat atgtcagctt tgttgattta
     5521 gtttatggtc ggtaaagtta ctaggaaact aataaccata agaaatagta cgtcatttaa
     5581 ttattgtggc actgctcgtg ttttgattaa ctaatacctg cgataaatat aaataaaggg
     5641 aagccaacat gagcaaaaat aagctgattt taacaggagc tttattgtct ttgtttgctt
     5701 tagtggggtg taagcctaca gacgagaagg ctattgctct gggccaacaa gcaattgcgg
     5761 atgatatgaa agatcccacg tcggtgatgt ttaggaaaga taagtttgtc aggactgatc
     5821 atgacgatgg gagtgtgaca ggctttgtgt gcggagaatt gaacgccaaa aatggctttg
     5881 gtgcatacgt gggctatcac tcatatgttg ttgaactcga aatgaagcca aagggcatgt
     5941 tttcgaaagg ggtggtatac aaaatcctat cgaagtcgtt ggcacctaat gatcctcatg
     6001 acttacagcg ttataagcag gtctacagaa agatgtgcaa cagtgaggct gtagctcatt
     6061 aatggtgaat gccgggcaga ctataagccc ggcatcttcg tatagggaac tattgcgcct
     6121 aaaatgataa gtagatacat acttagtttt ttgtaagaat actttcatct gttagctagg
     6181 agttggtatg aaagacgaaa ttcagaaatt ggcctgtgac atcattgata aaacaggttt
     6241 agaaatcagc gagggcagtc gactagacat cattggaaag gccgtcaata cagcaatgaa
     6301 tcatatcgct gctcgtatgg acgagatccc gctaccaggg ttgccttatc tgaaagttga
     6361 gttacacgta tggggcgaac cttcttgtgc acggcgttct gcattagttg tttttattag
     6421 caaagaaaac ccgctcagtc ttaaagtgca ggttggagca tggcttgatg gcagagtgat
     6481 ctacacaaat accgtttttt gtctttcaaa cgacgaaact attgaagcgg ccattcaaga
     6541 agcagttcta gcaatgcgca gcagcggttt gatgaagaat aactacgaag agtacttgcg
     6601 ttcgataagt ggtgaaaaga cattatctct gaaagcagat ttcgttaccc cgaaaaatct
     6661 gttggaagtt ttgattaata aaggggctaa tagtgccgta aatgtaatca gagagagtga
     6721 atacgcgtct ctttgggata tgtgcaaaag ccagttggat cttgtgcata tcgttgttga
     6781 tgcggggaag gcttgtgatg gcgtaatggc ggaatttgct gggaagatgg tcaggattgc
     6841 taacgaatta ccgatgatta agcaagaggc taaatcatac gccaccaatc atgtcacaga
     6901 gcttcttgcc ccttatcgct tagaaagcga tcagcgcaag atgattagct ggggaagttg
     6961 gtaagctctc tgtgtgtcgt tttttacgca aacaatgata ggtaagcaca agattatttc
     7021 tggcggtaat tgtgaaagct gatttatcaa aaatcccttc tatttcagga aataacggtt
     7081 attcacttcg ttgtgaggaa gtaaagataa acggtgagtc ggcatattgc agctattccg
     7141 tttgccagca caccattctt gccttcaaag aaaaccgtct tcctcgaact tctttccagt
     7201 cgtgcgcaac cgctattaaa gcaggcaaat gcaaggcgtt aaaaatgatg gttgaagaga
     7261 ttcgtaaagg agaatctctg tatttcgaag atatgaccgc actcattaag gaggttgaag
     7321 aacggaataa acaagccaga actataaaac gaaaacgtga cagtgtaacg attaatagca
     7381 tggttaagaa gagcgccaca tcacaaacag cgatcactga cgtgtatgcg gcgttgcttg
     7441 aagaaacaac aaaagaaaca catgagcaaa tcgatcaaca tatggaggca aaacaacaat
     7501 gaaaaagttg atcgcactta agcataagct ggacgaaatg aaagctatgg gaaccaatgc
     7561 aaaaaaagag gcattggcca acatggatga cttcgagcaa agcatggttt cattgatgct
     7621 caaccctttc atccgttttg gggtaaagaa atacaaagtg gtagagccgc ttagtgagtc
     7681 cgtcccaagt gacgaaaaag ccattgatat actgaataag ctggcctctc gcgagctgac
     7741 ggggaacgca gcaatagctg ctgttgagtc tatcgtggcg tcaatgtgcg ccgatgggca
     7801 ggacgtcttc cgtcgtttcc tcttaaaaga tccgaaagct ggcgttggga ttagcctatg
     7861 caacaaggtt tttgaaaatc ccattccgaa attcgaggtg cagctggcgt caccgtataa
     7921 agaaaaaggc gacaaatacc ccttcaagcc aaaccctaaa gcaaaatggc cgatgattgg
     7981 cagccttaag ctcgatggtt tgcgagtaat ttgcgaggtt attgttgacg aggaagaggt
     8041 taacttcctt tctcgtactg gtaatccaat cacgtctctc gatcacttaa agccagccat
     8101 gctcgaatta ggcaaacttt caggccacaa acacatcttc ttcgatggtg aaggaacagc
     8161 cggttcattt aaccagtccg tatctgcatt gcgcaaaaag aacgtgcagg caattggcgc
     8221 tatttatcat gttttcgact tcttcctacc ggaatggcga gcacaggcta aatccaaaga
     8281 gtatgcaaag acaggtatga agctgaaaga gcgcctggct atgctggtgg cgttgttcaa
     8341 aaacgatcgc agtgaaggct acacacaaga cattcacctg catccgttct acatcatcca
     8401 tagccacgaa gacttcatcg aacgcttcat gaaacgcctg gacgataacg aagaagggga
     8461 gatgggcaaa gatccgaact ctgtttacga gtttaaacgt acccgcagct ggtggaagtt
     8521 aaaagacgaa gactcagaag atggtgaaat tatcgacttt gagccgggcg acccggactc
     8581 tggttttgcc aacacgcttg gaaaaattgt tattcgcctt gaaaacggtg tgattgttcg
     8641 tgcgagcggc attaagcata aatatctgga cgagatctgg aacaacaaag agaagtaccg
     8701 tggtcgtatt gtcgaggttc attgtcacga gaaaacgccg gatggcagct tacgccaccc
     8761 acgactgaaa tggccgcgtt gcttacgcga taccgaagat cgaatcggag ataaagaatg
     8821 atcgtattaa gtaaaaggga gaaagaaacg cttcatgaaa tcagtaagtg gccggagttc
     8881 cctgagtact ggaagcctaa aacgcgagct aagttagagc gtttagggtt ggttgcaaac
     8941 gtttctgaaa cgtggtgctc ggccaactac cagttaactg ataaagggaa agtattgcta
     9001 cagcaattag tagaatcagg agcgttaaaa tgattccata catctcatta gcttttatgg
     9061 gtggcttcct tattggcttc ggcatctgtc gtgatttaat taagcaggaa cttaaaacca
     9121 aaacactgtg catcggaaag cgtgtgtatc gggtagttca tgaaacaaag gttagaaaat
     9181 gagcaattta acttcttggg actggtggtt ggccacctat ttcttagcgg ccggagtcgc
     9241 attcgccttt tacgtaggtc agttagtcgt aaaactgttg ctgataaaat ttgctagtca
     9301 taaacgtatc gatgatggtc tgtggcgtct tggcactctg gtggaaactc gctacgggca
     9361 acttaaggag aacgaaacca ttactatcca agcgaaacgg ttcactgcca ccatcacaag
     9421 aacacctagt cgtagagtgg ccttgatcaa aaaagtcaca accgaataaa aacacaatga
     9481 taagtattta cttacttatc ttttatgtat aagatgactt tgttttcgtt gagacgcgac
     9541 tgtttgaact taaatacaag tgcaaacgaa gaagtctatc tggcagtagc ctaataagcc
     9601 aaacaccagc gaggtcagtt tccagcctcg ttaccgaaat gggacacact gagcgagtgt
     9661 gattgcagaa cgcaggaggg aacattcatg ttccctccga tgaagtaaca gaatgggcgg
     9721 ttggtatatt ttcaactcca tatgactccc ggattcttag ccactgaccg cccatcctgt
     9781 tacgtcattt tgttcaatta tgtcgtttat actgggttaa aaagcggcga cgtagcccgg
     9841 ctggtatggt tagccagcac acaacgttga ggccattaca tttttatcaa ttctaaggtt
     9901 ctattcacag agataccggc gagcgttgat atgtaacatg ttgggcaaac attcaatcgg
     9961 agtagtggcc tcaacgttgt gaagacagga ttgttgtgta ggtttaacca ctgttgccat
    10021 tggtgcctgt tttcacaaca aatgattcca tacatcacat tgtataaatt acaaagtagg
    10081 tgctgtcctc agaaacatca tctacttaaa gattttgcct tctactattg agcgaagtcg
    10141 aaagcgtctg gcactaacga aaagtgcaag tagcggtgcg tttcctggca gaaactaaac
    10201 cgtcgcgatt ggcactgttg agtaataaat actggcagtg ctgaattgat ggtgtagctc
    10261 agcggtagag cagttggctg ttaaccaact ggtcggtggt tcgaatccac ccaccatcgc
    10321 caatttaggg gagttagtcc gtaggggcag cggggtagac tgtaaatcta ctgtcattgc
    10381 gactcgggtg gttcgactcc atcactcccc accaaattgc cggtttagct cagttggtag
    10441 agcgcctgcc ttgtaagcag gatgtcagcg gttcgatccc gttaaccggc accaacacaa
    10501 caggtaagag cattgggcga atcggcgata ctgacccaca agcccgtaaa tcgatagagt
    10561 cagaccagtg ctcttaccgt tgtgatgaat gcacaggctg atgtgccgca actacagtag
    10621 tgcgcgcttt gcggggcttg ctacaaccct gtgtcggagt tcagcaccga ccatcacaat
    10681 agctggagag tagggagcat ggtgctcaag cggtcttgaa atccgtccca ttgtgcaaac
    10741 gatgatggtt cgattccatt actctccgcc agacacagcg ttgagcggtt tggcctttta
    10801 atcacccaga ttaagactcc gctaacataa accagaccgc tcaacgctgt gatagacaat
    10861 tacggcagac gttcttaacc atagcttgct aacatcctag caacactttt tttcagcgca
    10921 aaattcaaag gggcttcggc cccttttttg caactgaaag agtcaagtct acgaactttg
    10981 taggaagaaa taatgaccag caaagagaac acaccactaa aagtcacccc tgaacacatt
    11041 gaacaactca ttcaaagtga acattatttc actgcatatg atgccagcca cggggataac
    11101 ttcatctctg tttacaattc aaaaacagat atcgacaaag gtcatgagtc gttaaaactc
    11161 ttaacgttct gcgttatggt tctcaaaaat ggatataccg taacgggaaa atctgcgtgc
    11221 gtaagaccag aaatcttcaa ctttgatgtt ggtcgcgagt acgctcgaaa agatgctatc
    11281 gatcaaatct ggccactgga aggctatctc ctgaagcaaa agtggcatga ggcaaagcaa
    11341 tgacgatcac tatctacgga cgagataact gctcatactg caaacgtgca gtcgagctgg
    11401 cgaagcaact aaagggacat ggctacggtg attatgagta catcgacatc accactgccg
    11461 gtatcgacaa ggaaaaacta agtgaaattg ttggtaaacc ggtagagact atccccaagg
    11521 tgctgatcga tggccagccg attggcggat acacagaact ggctgcatac gtcagcaccc
    11581 tctgatttta acggctcaca ggagccgttt ttattcccac caaactcact cccatttccc
    11641 ttaaaattca aaaaacaacg tcaaaatgat tccataccta ctatgtatgg aatcattgct
    11701 gaaaatgagt tacttttact cttgatccta taagaatcta tgcctaatat actgtttact
    11761 tatacagtgc atcggcgtaa ctcggtgatt gtcatatgaa aaatagcttt gacagagcac
    11821 gcgctgcgga gaacacctca aaagaggcga tagagtatct cgaaagagca tctcaaatgc
    11881 aggccgttat gatctcccag gtcagcaatg acatgagatt ctcggacgca ttcatgttat
    11941 tcactcgctt atctctgctg ataaccagac gtcggccaga gatcgctgtt cattgtattt
    12001 tgatacatgt tttgccgcac attgccgatg taaaagtaag tgacattaat aggttcatgg
    12061 tgaaccaact ggtcaaccca ctaatactgg atggcaaaat tgttatgggc cgccgcgttt
    12121 tctctctgat gaagcagttc cttagctggt gcgccttcca ggggatgata gacgtgtcac
    12181 cgttaaacga tatgtcactt aacaaagttg ccggtggcgc aaagcccaca cctcgcgagc
    12241 ggaagctgac cgacgcagag gtatgggtgt tctggaatat atgggactac ttcaatgtgt
    12301 gtgctggtac aaaatgggcg gccaggctat gtcttgtatc cgcaagacga cctgacgaag
    12361 tactgcgggc taaaaaaagt gagttcaatc ttaagcgtgg ggtttggaat caaggcaaga
    12421 ggaacaaatc tgcccgtgag cattctctgc ctttaagctc attaatgcgc acttgtattg
    12481 aagagttgtt cgaatatggt aaagacagcc agtggctcgt gccttcgaat aaaaaaatcg
    12541 ggaaagacct tcctatgtct aaagtggcaa tagcccaggc attacgtcgt attctggaac
    12601 gaccagaact gatggagctt gagccattta caccccgaga tttgcgccgt actgcgcgta
    12661 gttacttccc agcattaggc ataagccagg aggtatcacg taaaatcatg aaccacagtc
    12721 ttgaggggat agatcgggtc tacgaccggc acgattatat ggacgagatg cgagacgcct
    12781 tagaaagttt ctcgacgtac atcgcatcaa tcgtagagca accggattta gacgaaattg
    12841 accacaaatt taagggagat cgtctatcaa cagagcttat tcgtgtaaat ttttcataga
    12901 gactttatgg cctcaacaac cttttgtgat gcgcctttct ctttaccgaa tcgctcgtta
    12961 tatgcagcaa gaacctgttt ttcgtcctcg ttaagaggag cagtgccttc tttgtataaa
    13021 aatgctgcga gttcgggttg gcgttcttcc agcaccatca tcataagacg acttggctca
    13081 atacccagtg ccagcgccag cggacgaacc ttatcgatag gcaaaggaat tttgccgctt
    13141 ttaattaaag aaaggttgtt ggcgttttta tacccaattg ttttggctat ctgggcctgg
    13201 ctcataggtg aggattcaat caaccctgcg ataaaagcag cgtagcgact ttctataaat
    13261 tcaatcttgt tatcagacat ggttacaacc tttgcgcgtt caattctctc tggtaagtgc
    13321 ttaccgatat tacatcaaag gttagggttg taaagctatt atcatttttt tcgataggca
    13381 cttaaaagac cggttaaagg ccattgcacg gagaaaaatt agcccaaaac aggtaagaaa
    13441 atcaacttgc atatgatatg aatgtattca gtattgatac aaattttagt agtattcctt
    13501 accatagtat aagttagaat ggattgattg aatgaacacc actatttcca gcctaatcgc
    13561 tcttgagatc ggacacgtac agaaattagc tgatgagtgt gtagctgaca tcctcaccga
    13621 tctaccgaat gagcagattc aggttggtgt gaatgacaca actggattta tattcgaact
    13681 taacaacaaa cgcttcacgc ttctcaatac cggctccggg tctttagccg tcagaatctg
    13741 ttaacccctc ttctccctgc gcgaatggct tagttccctg ttcgcgcagt gctacattaa
    13801 acacactagt aaataatttg ttttcataac aaaggattag ccatgtctaa aaaacgttcc
    13861 atcaaagagg ttcaggactt ccgtgacagt gtaaaacgag tagtcgctct cctttcaggt
    13921 aaaaacatcc ctgttgcaga acgaggggac gacgcttatg tatgctataa cgatgatgga
    13981 gagccaattc tcgtaaacat cccatcaatc ccggataacg caacaccggc attgatgaat
    14041 gctgtgcgcg gatttctcga tcatgaggtt gctcacattt tgtttaccga tattcgtgtg
    14101 tccaacaaaa tgagagaaaa aggacgcgtt ccttcctggt cgctatggaa tgccttagaa
    14161 gacgtgttca tcgagcgaaa aatgggtcag gtctttaacg gaacaagacg taatctgatg
    14221 gcaactcagc gccttataat cgaaaaagtc tttaaaccaa aggcttcaga ggctattgct
    14281 tattgtggca aagatcagcg cgcgcttttt ctaaacttct ttctctgtcc ggttgtaaga
    14341 gcctgggatg gccaagcacc gttcgtagat ttcatggatg aatattggcc tgtcattgag
    14401 aaaccaattt cattattaaa agaacatggt atcgatgtgg ccgtgcgtaa catgtcttgc
    14461 accgaggatt gtgtaaaggt ggctgcgacc atagctaaga tcctcaaaga cactgaaagt
    14521 gaaagcaaag gtaaggagtc agctccggga aaaacttccg atccttcaga cgctgaacag
    14581 acggatgcct ctggagaaaa caatgaagat aacgaagatc atgagacacc ctcagcgtta
    14641 gataatcaca aatctatcaa atcagaatca cacagtaagc acaaacatga taataacgac
    14701 ggtgatgatt cagataattc tgaatcatca gaaacaatat tcgatgatac agaaaatgat
    14761 aaagaggtat cagattctga tgcttctgat aacgcggtgt cagaatcatt taccgctgac
    14821 cacgaaaaaa gaaaaacgac agaagacggc tcttcagata tcccaactcc gtcaaaaatg
    14881 agtctggaag aggctttaga ggagctggat agcatagaag atgaagtcgg aggcatgaca
    14941 gaagatgctc tatccgaaac gattaaaagc gagttaacag aaagctcgaa aagcgaatac
    15001 aggccataca atcgctcata cgacttcatc ggctcgattg atcaggcaga agcccatatc
    15061 aaacggctta ttaaaacatt ctccgatatt gatttaggag gatatccaat cagccgctat
    15121 cgcatcgttc ctgaaggcaa ccagctcttc gacaaatata ttgaaaagca tctttcgtca
    15181 ggtgtttcgt cgacgctggc aaaagacctg gagcgagcaa tagcaagcag aaacagagtt
    15241 cagtttatac cgggccagcg tcgggggcgc attcatggtt ctagtatcta cagattagca
    15301 atgaatgatg atcgcgtgtt tcgtaaaaaa gaagaatcta aagccgttaa cgcctgtgtt
    15361 caacaagtga ttgatttatc aggttcaatg agtggtataa cgatacaatt ggctcttgca
    15421 agtgcatata ccatcgccga tgcccttgat cgaataaatg ttcccaacat tatcaccggc
    15481 ttcactacat ttggtagtca tatggcggca ggagaactta aggctgtcaa gtatgagttc
    15541 tctcgctttg aatctttaat gctacctatc atcaaaaatt ggaatgaaaa ggtaaattct
    15601 cgcgaagttc gctcacgtat ggggtgcgta ggctacacat tcccacttct taataacgtg
    15661 gatggtgaaa gcatagccag ccttgcatcg ttattttccg gtcgcatgga ggacaggaag
    15721 atcatgcttg ttctgagtga tggcgcgccg tgggctgttg ggagaggttt tgacgctcat
    15781 ttgcgttcgg ttgcgaagca aattgaaacg cagactgaca ttgatttgat ggcaattggc
    15841 atcatgactg acgcaccgga gagattttac tcaaatcatg ccctggtaac gagcgttgat
    15901 agtcttggtt catctgtagt tactgaacta tctcgtatca ttttgaagtg aataaaacag
    15961 ccttaatgat aagtaaccac ttacgatagt taatggtata tttatataag aagttgaacg
    16021 ctcattagag aacaaaggaa aaacgcatga cgactactgc actgcaaaat gaaaaaaatc
    16081 attctgatta ccttgtttgc aagtggtgcg gcaaatcatt tcactatttt aagtcccatg
    16141 tagccaatgg taattgcgag ggcattcctg agtcagtaaa agatgccgat cctgacaccg
    16201 tactgaaaat gtacacaacg cagtttccag atgagccaac gctatcgaaa aaggcacttg
    16261 atgcaattca agctaaacgt gccgagcaaa aaagcgaaat ggccaaatct tctggcttga
    16321 ccagtagccc tggctacaca ggcacagttg agtacaagac agatctggtc gcagctcacg
    16381 aactgctaaa cgtaacggtg gaagaactcg gaacaaaacg tgggacgccg ctcatggtta
    16441 gcgtcaacgt caatacgccg tatccagagt tcgttccaga agtgaagaag ggctacgtat
    16501 atggcgactt cgaactgatc aaagatattt tcatgatgct tgaacttggc atacctggct
    16561 atttgtgggg tcatgcagga acaggcaaat cgtcattgcc tacacagcta tgtgctttgc
    16621 tcaatcgtcc gttgatccgt gcccaacata cagcatcaat ggaagaggca catgttacgg
    16681 ggcaaattct ggcgcgtgat ggctctacgt atttcgagcc tggcttgctt gcgctcgcaa
    16741 tgaagcatgg ctgggtttac ctcgcggatg aatacgactt tgcgtttcca cagattcttg
    16801 gcgtgtatca gccagtgctg gaaggtgaag cgttggtcat caaagaggcg actccagaat
    16861 ggcgtcgcat tactccgcat gaacggtttg ctttcattgg cactggcaac acgaacggat
    16921 ctggtgatga aaccggcttg taccagggta caaacatcca gaacgccgcg aacttttcgc
    16981 gttttggcat cgtttcgaat gtgaaataca tgagcaaaga ggcagagatc aacatgttga
    17041 taaatgccgg tatcgtgggt gaatacgcag aaaagatggt taagtttgcc ggtatcgttc
    17101 gcgatggata cgaagaacac cttatcagtc agccaattgg ccctcgtgaa cttttgttgt
    17161 cggccaagat tggaatgatg cgaggcgact ttgtgacagg tattgagcgt tctttcatta
    17221 acaaactccc ttcagcttct gcacaagcgg ctcgtgaagt tgttcaaaaa atatttggtt
    17281 gatcgtgcgt aaaggatgtt tcggctctct tatcgctgct tctgaaactg gtaaggcttg
    17341 tctggtgtgt ccagacaagc ctgattgtca ccaatcagca aaagaagttg cgatttcgat
    17401 gcatgggaag ttcgtaggct tcccaaatga caaaatcaaa aaaaccagaa aggtaaaaac
    17461 acatgaaggc actgatggtt cgaactgact tctcacttgg ggagtcggct ttaaaagcag
    17521 aaaacgcggt gaagattgcc agagaagctg gctacaccgc tgtaatttca gcagatagca
    17581 tgaatattgc gagtgttatt ccactacaac gtgccgctgg tgacgacatg gcggttattt
    17641 gtggtgtgaa actaaatatc gttgatgatc ccacatacga gcaccgggct aaacttgcta
    17701 aagaatctaa gggatgtatg gaatcattag agcgaggacg taactactcg tttaccgctc
    17761 taattaaaaa tgagaaagga tatcgcgaca tctgcgaact aatgacggcg gccaacacac
    17821 gagaacagtt ctactttgta ccgcgtctct cgctcgaaca gttggtttct acatatgcca
    17881 aaggcaacat catcctgctt acttccgaca tcggtagcgt gttccaacgc aacgattttg
    17941 caaaaatcat aagcacactg attacagcgg gtggaaaaga caacttctat agtgtggttt
    18001 atccgcaccc taccccattc tacgaccaga ttaacgtccg ggcgatgaaa gtcgccagcg
    18061 cattgaaaat agagccagtg gcgttctatc ccgcttatta cgaatcgatc gacgatgcag
    18121 acattaaaga cattgcgcac atggttacga acaacatcaa aatcgaccag ccgcatcgtc
    18181 tgcgtatccc ccaccagcga gataacgccg tcaatggtcg ccgccatctc cttgaggcgc
    18241 ttaaagcctt ctccgttcgc atggatgtgc cggtaacagc tgcaatggcc tcaacaacgc
    18301 aggataccat tatcgatgcc tgcacatggc gctggcatga attgccgcca gcactgccaa
    18361 agatggcaga cgacgagcct gcaacgctta tgaaactggc tgttgcaggg ctgcgtaaac
    18421 gtcttaccac aaaagagttt ggctacacac cacctgcttc tgagaacagg gtttatgttg
    18481 agcggctaaa gtacgaaatg gacacgctga ctcgcctggg attttgtggt tacttcctga
    18541 tggtacgcga tctgatgaat cacagccgtg aaactggcat tcctgtcggg cctggtcgtg
    18601 gttcctccgc tggctctttg gtggcatggt gcataggcat aaccaacgtc gaccctatcc
    18661 gtcacggtct tctgtttgaa cgtttcatca accctgagcg tctcgacttg ccagatgcgg
    18721 atttggactt cagccaggca cgtcgccatg aggtgatcga gtatctgaat gaacgatacg
    18781 gcgaagatta cgttgcgggt attccgaact tcacatatct gggcgcagcc tctgcactac
    18841 gtgacaccgc tcgtatttat ggtgtggagt ccgcagatat ggcggtatca aaagagctga
    18901 ggaacgtcga ggatgatagc cttccattgg aagagctgcg cgaacaactg gcaagtctcg
    18961 acaaatacgc gacaaaatac cctgatgcat tcaatgctgc ctgcaagtta caaagtctta
    19021 tgcgtggctt tggtagacat gcggcaggga tgatcgtagc aggtgttcct ctgacagaac
    19081 gtacaccggt tgaacgccgt ggtgacgcgc gttgtatcgc atttgacaag cgttactgcg
    19141 aggctatggg cctaattaag ctggacgtac ttggtctggc aactctcgat ttgctcgata
    19201 gtgcaaaacg ctacataaaa gagaacacag gtgaagatat caatcttgat gccatttctc
    19261 ttgaagatcg caaggtgctg gatggttttg ctgctgggta cactcaaggt gttttccagc
    19321 ttgaatcagg cccaatgcgc aagctgctta aagatttagg tggtggaatt gagccaatga
    19381 gctttaaaac ggtcgttgct acgactgcgc tcttccggcc ggggccaatt cagtcaggca
    19441 tgttggatga ttatgtttct gtcgccaaag gctttatgac gccggaatca ttacaccccg
    19501 ttcttgatga acttaccgca gaaacaaatg gcgtgattct ctatcaggaa cagacgatga
    19561 acgcgactcg attgcttgcc ggcttcacaa tggctgaagc tgacggtgtt cgtaaagcga
    19621 tcggcaaaaa agacatggag aaaatgaaga gcatgggtga gaagttcatc gttcaggctc
    19681 aagctggctg gatcgacgtt gagctggaag atggcactac acagcgcatt caccgtgcgg
    19741 aacattttaa atgcgaagac ggaactctga aaactgtcga agaggcactt gagttcggtg
    19801 caaaactacc tataaacgca gtacgcgtta cagcgtcaca tccagggcta tcagagatga
    19861 aagcgaagga gatctggacc gcattcgaaa agaatggtgc ctaccagttc aacaaatcac
    19921 actctgttgc ttactcctta atcagttatc aatctatgtg gttgaaaact cattatcccg
    19981 cagagttttt cgctgctgct ctcactattc ttggcgaaga taaacaccag gggctggtta
    20041 aagatgcgct gacctatggt attcgcgtat tgccaccaga cgttaacgtg tcatctaacc
    20101 gaatcgagat ccgcacgctt gaagatggca gccaggtgct gtatgcgccc ttctctgctg
    20161 tgaaggggtg ttctgagaat ggctgccagg ccatcatgag agcgcgagaa aaggttggcg
    20221 gcaaattcga gtcacttgcg caattcgaag aagctgtcga gaaacgtgcc tgtaatagtc
    20281 gagtgcgcga atcgctgcaa aaagtagggg cgtttgcatc catcgagcca ggtagtctgc
    20341 cagcaactga tccagagcgc ctccgcgacc aggctgaatt gatgggaaac cttgtcatag
    20401 atgcagttaa agcctcacgt ccgtttgaga tgaaccccaa acgttcggct gagattaacg
    20461 tgctcatgac acggatggcg gctgaaatgg gcttaggtga tgaactaatc cgccccagca
    20521 ttggtattaa gccgaaaatc atgatcattc tggacaatgc aaacggcaat gatgctcgaa
    20581 ccggttactt catggagaac ggatacgacg attttaaggc caagctgttg acggctggag
    20641 atttgcgcat gggcgatctg tatgtcacag gcgtttgcaa aaaggtgaaa gacaaagaaa
    20701 aggactacac caaagacgag atcggccagt tcaccgactt tatgcgtgaa gagatcaatc
    20761 tggtacgtcc gacctatgtg ctgacgtgtg gcagtcgggc aacggcattg tttaacaaca
    20821 aaaacaagcc atccgattta gtcggtagga aggagtatct tccagacctt gacgttacag
    20881 tcttttacgg gtttaacccc aacattcttt acttcagacc tgaagaggga gaacgactag
    20941 aagcgatatt agccgacgta gcagaaacta ttaagtcgtg atccaataaa catggcctct
    21001 atggccatgt tttctcttat cccctcatat cccctatcct cgaactcctt gccttatcat
    21061 cacaatgata taatcaatat aagatgataa gtaaaaagga aagcacatga tcaccgatat
    21121 ttacgagaaa ataatgtctg atctcgagtt tgaccgagac aatctggagg aagtctggcg
    21181 tagacaaccc cgccttttaa tggagtatgg ctcaaaactc gctcatgcag aaagaagtgt
    21241 cgcagaggca aaacttaacc ttgaagctgt tgaagcaaag ctatacgaca cagagcgtaa
    21301 gaacttgagt atgaacggca ttaagttcaa cgagtctgta ctggacgcta aggttaaaac
    21361 aaacccacag tatctatcta aacgtcagaa gttggatgac gcacggcaca tagcagacat
    21421 atacaaacat gctgtcgccg ccttttcgca tcgccgagac atgatcgttc aggcgtcgaa
    21481 gatggccatt gttgaattag agcgattagg ctccgaacgc tttattactc cccgttgatt
    21541 tttgatagat aataagtaag tactgatcta tcatttaaca gctcgaaaga gccacgaatg
    21601 aacgaaagcc caacgcgcat agcgccatcg gccaaatcac aacaaggaga aacacatgtc
    21661 taagacatta cttgatttgc ttaacaaaac tcgtgaagac attgccgcca aacgtggtaa
    21721 caacgttgat ctgactcgct taaaagacgg cgtcaactat atccgcatct tcccgaataa
    21781 agacgaccca aacggtaagt tcttccagac tttcggtatg cactacgtta agtatcagaa
    21841 cgaggaaggt aaagaagcaa ccaacgctta tatttgtgag caacatactc acggtcgcgc
    21901 ttgtcagcta tgcgaaatgg tgatggaagg tcgcgctcgt cacaagggta acaaagcaat
    21961 ggaagaacgc atcggtcaaa tgcgtgccac tcctcgctac ctggtcaacg gcattctttc
    22021 tgctcgtgag gatttcgcag atgctgagaa atgccagtta atcgagctgc cgtctactgt
    22081 attcgatgat atctgcaaag caatcaccga agacatcgct gatgatatcg gcaatccact
    22141 gagcaaagag gaaggctacg cattcctgat taaacgtact ggctctggtc gcgataccaa
    22201 atatgacgtc tcgcctaagc gtaaagtcta caaaggcgat atcgaagata aattctggaa
    22261 cacccagcat gatctgatcg catacgcaaa tcaggctgat gaaactcgtc ttctgtcgac
    22321 agttcgcact atgggtcgtc tgattggcat cgctgcacca actgccgcag catctgcacc
    22381 agcaatatcc tcaaccgcga aaacatcggc tgcggcacta cctggatttg gctctgtcac
    22441 tggtcataca gaaggagcga cggctgtagc aaccgcgcac acaccggctt ctgaaccaac
    22501 cagtctggtt gatgaagaaa tcctccgtgc cgttgaaact gaatttaaac cagaggcaag
    22561 ttccgctgcc gttgccgtat cagtcaaaga gtctgaagca gtcgcagcga catctgtagc
    22621 aaccgcatct gcgacggaag atgaaggtct ggatgaccta ctgagagagc tggactctct
    22681 gtaatcccat tacgtgacca gtaaggcgtc tacggacgcc ttactttttg gaaggaatgt
    22741 accggtgaat tatctcttcg tagatggcaa tagcctgggt tattaccacc aacaatctga
    22801 caaattgcac aacggcgaaa tggaagtaca ggctgctttc ggctttgtta agaacgtccg
    22861 tcgttatgcc tccatcctcc atgcccgacc tatgattctt tgggatggat ttagtgacaa
    22921 gcgtcgcgac ttttacccgg actacaaagc aaatcgcgac gacgatcctg atatgaaaaa
    22981 gatgaaggaa ggctttgcta tccagaagcc atacatcctc aaaatgatga ccgcgcttgg
    23041 agttacccaa ctcattgcaa aagatgcaga agcggatgat ctggccgggc tgctggtatc
    23101 ccgcatggca ccgcagccaa ccgttgaaca catctatctg ttaacaggcg atagcgactg
    23161 gcttcagtta gttcgtgaaa acgtaagctg ggtaagcctg cgtgaagacg ccaaaaacaa
    23221 gcaggttaat tttgagcaat ttgcggagct gacaggattc gccacgcctc gcgcattttt
    23281 ggaagcaaaa gcattacaag gcgataactc ggacaacatt agcggtgttg gtggcattgg
    23341 tgctggcggt gcgaaagagc tgctgcatga atggggaagt gtcgcaacga tggtacgcgg
    23401 catcaacgac ggctcaatcg tggttaataa agggcgtcat aagaccgcct tcaacaaact
    23461 agcgaagaat gccttcaacg agaaaacagg ctgtcgaatg ctcgaagcgt tcaagagaaa
    23521 catcacgcta atgaacctga ttgagacgaa gtttccgcct accgaaatcg aaacaatcaa
    23581 aggcaatcgt gacgtgaaag cattcgagca actgtgctac gagctgaatt tccgttcgtt
    23641 ccttgaagac cttgaagtgt ttgttcttcc attcgaaagg tattgctaat gcttaaatcg
    23701 attatcaatg gcgctacaac cacccctgcc caactggcaa aagagattgt cttttatcac
    23761 ggtgagtacg ctgtcatcgc actgccgtca attctaggcg ctgccggaat gaaagcgaca
    23821 gatcgcgagt ttggattagt cagcgagcag gtcgtaaaaa tcctcgctcg tgtatccaga
    23881 ctccttaacc acgatgcgat tgtattcgat gaatccgccg ctttaaaacg aatcaacgaa
    23941 acaaaaggag cctgatcatg gcaaaaggaa aatccgcact ggcacttgct ctgaaaaaga
    24001 aaatcggtag caacgacgaa attcagaaag taactcattg gattgataca ggctttccac
    24061 cgttaaacaa agctatttct ggtcgttacg atggcggctt cccatgtggt cgtatcgtcg
    24121 aagtattcgg tcctccaagt gcagggaaat gtgttaccgc agacaccatg ctgctgacgg
    24181 agcgtggaat ggtaacagtg aaagagttgt ttgagattga agggtacaaa gcgacatgca
    24241 ctactcgcga tgtagagcat aacgttggac tcatcaatga aaatggcgtg atagagaaga
    24301 cctcacacct gacatggaac aaccgtcgca aattcaagcg tattaagctg gcatcaggcg
    24361 gttatatcga ggctacgttc cgtcacccaa tccgtgtggt tgacgactta ggtaatgtcg
    24421 tctggcgaca tgctgaaaaa atcagtgtag gcgacacgat tccttcaatg gttggcacac
    24481 atcaattcgg cgatcagcac ctggatgcca atatcgcaaa actgatgggc tatttaattg
    24541 ctgacggata cgtggcctct gaaaattcag tgaatttttc taacacagat cctttcatca
    24601 aggatgagta ctaccgcctc atttcgctgg tatcagacaa gatgccagtt acgagaaaac
    24661 ataacggctc ggaagaccat gtgctgttta gcaaagaggt gcgttcgctg ctttttaaag
    24721 aatatggtct ggagtatgag aaagctgctg gcaagcaggt tccgttgagt gtgcgtcgcg
    24781 ccaatagcga ggctcaaatt gcattccttc gcggctactt tgagctggaa tgccacgtca
    24841 atgatggtcg ctgcattgag gttgtgagcg cgagtgggct gctgctacag caaattcgcc
    24901 tcatgctcct gaatctgggg attacgtcaa ctatctctga aaaacacgtc gcgggttatg
    24961 aaaatacata ttaccggctg tcattcagtg gctctaatta cgaccttttt ctgtcaacga
    25021 ttggtttcga atctccggca cgtttatcag tggcaaccaa acgggacatt ggttttgacc
    25081 gcacttactc aggctatgtt ccgcacatca gcggcttagt gaaatcactc tacgagtcgc
    25141 tcaccaagac cacccgtgaa gactacgctc tggtagacca cgttattggc cgcggcgatc
    25201 gtgtcggaat agacaaactg cgagaaatct atgtctcctt cattggcaga aagaatcgtt
    25261 ttaacgagca tctgtttgca caactggcag cggtaattgg ctctaacttg ttctacgacg
    25321 aagtcgtggc tattgaggaa ggtgaagcac caacgttcga cgtagcgatg ccggaaacac
    25381 actctttctg gtctaacggg attatcagcc acaacacatt ccttgcaaca gcagcgatga
    25441 tctccgctca aaaacaagat ggtctggcgg tattcctcga tcatgaaaac agttttgacg
    25501 ttggcctggc tgtagccaat ggcctgaacg cagatgagga tgacggtcag tgggtataca
    25561 agcagccaga taccttcgaa gactctgtag agttgatcgg cacaatactt aaattggttc
    25621 gtgatgaaga gcttatcccc gaatcagcac ctatctgtat cgtggctgac tcacttgcgt
    25681 ctatggttcc gaactccaaa gccgagaagt tcgaaaagat ggctgaaggc actgccaaag
    25741 acaaagatca gctaaacatg aacgacaata cggcactggc tcgtgcgaca agtgcgaact
    25801 tccctactct ggctttgtgg gcacgcaaat acaacgcctg catcatcttc ttgaatcagg
    25861 ttcgcacaaa aatcggtgta atgtttggcg accctactac gtcgccaggt ggagattcac
    25921 cgaagttcta cgcttctgta cgtatccgtc ttggtgcatc ggtgatgaag gatggtaaag
    25981 agaagatcgg ccaggacgta ggcgcagaat gcatcaaaaa caaagttgca ccaccgtatg
    26041 gcaaatgcac ctggaaattc tacttcgatc ctactcgtgg cctcgacgtt atcgaatcgc
    26101 tcgtcgagta catgctggaa gaaggatacc tgccaaagaa cgccagcggg cgagttgaaa
    26161 ttggtgacaa gaaatacacc aaatcgcaga tcgtcgagat gtatcgggag aagccactgg
    26221 ctgaaatcat tgcggctttg caggcaatcg acgaccgaag agcaaaagac aaccccaccg
    26281 agtcagtaga agagtaaaca caaggcgtcc acaggacgcc ttttttatct cttgaaaata
    26341 tataagtact tacttattat tttcgcatga caaccacata ggaaaacaca tgatcaaaat
    26401 ctatctattg gcagtagcca caggcctttc agtggctctc atctacggtt tactggttcc
    26461 gtcgctgatt tctaccaaga gtgatttagc cgtcatgttt ggagttatcg ttggttttgg
    26521 tgctcctgta atcggtctta ttgctggtcg taagtttatc aactcattaa tcaaagcaaa
    26581 ggggaaataa gtaatgaaga aaggtttact tgcagttgct ctggcggcta tttgcacaat
    26641 gggtcttact ggctgtgatc gcgtggagcc tggatacgtt ggcatcaaag taaacaaatt
    26701 aggtgaagac aaagggatcg gtgaagtggt tggcgttggt cgccaatgga caggtcttaa
    26761 caccgaactt tacgtattcc cgaccttcaa acaaatgaag acctacgacg agccgttcac
    26821 attccagatg agtgacggta ctgctattgg tcacaaaatt ggcgttgcgt atctggttaa
    26881 tcgtgacaag gtaacgacgg tgttccagac ctatcgcaaa ggcgtagacg atatcaccga
    26941 atcagatctg cgtcagaaaa ttgccgactc tctaaaccgt ttggccagcc gtatgaccac
    27001 tgactcattt atcgacggtg gtaaggcgca attgctggac aacgcactga aagatattca
    27061 gaaagagatg tctccggttg gtattgaggt actgagcctg tcatgggttg gaaagcctga
    27121 ttacccaaaa actgtcattg aatctattaa cgccaaagta acggctaacc agcgtactct
    27181 gcaacgtcag caggaagttg aacaacgtaa agctgaggcg aatatgctac gtgaacaggc
    27241 taatggtgaa gctgatgcta tccgtgctcg tgcgcaagca gaagcagacg ccattcgtct
    27301 gcgcggtgaa gctctgcgtc aaaacccgaa cgttatggaa ctggaagcca tcaataaatg
    27361 gaatggccag ttaccgcagt acatgactca aggggctaac actcctttca ttacagtgaa
    27421 ataactcccc taaaaagttc aggcgtccag ttggacgcct tttttatcgc aattatctta
    27481 ttaagaaaac aatttgttta aaagaataag aaaacatgac agctattaag aaactctacg
    27541 atgccgcaaa cgtggctctg gatgttattg atgatgaagc agcaaaaggc tttcctgaac
    27601 ctgattgggc gcatcagcta cgaaacgcta tcgccgaaat gaatccacca gatccgacca
    27661 ccgacgagac agactggcag cgatttatcc gtatgtacgc tcaggaaata ggtccaacgc
    27721 caacggcaga gcaagcaatg ctgctgaaat acttcaaaga ggcgggagag gatttaccaa
    27781 ttgatgactc agcatattgg ttccactgcg catggcgtaa gtatgacgtg atattcacac
    27841 aaggcatggg gagcaaagat atggttgtgt ggcatctact ccatatagac acagccgttg
    27901 acagagttat tgaacagttt ttccctaaac aagaagattg atcgcctatt cataactaac
    27961 aaaataagta aacactaacc acaaaaggaa aaacacatga gagttttagt tcgaatcgtt
    28021 accagcactg tctatgacgt gtttccgctt tttatggtca aagccgatgg ccttaacgac
    28081 gaagaaactg acgcgctgat ccagcgtatt ctcgttgaat atacaggtca tgacgctgat
    28141 tcagtgatgg ttgatgatga tggtgtttgt tggcataacg gcaactgttg gtacgtagaa
    28201 gagactcaac aaatcagtga tgaagatgcc gcacatcttg agcgtatttt aagcatcagc
    28261 acttttgagt gagtttacag taaaatttat ataagttagt gtctacctat catgaagatt
    28321 tttattgaat acttgttact catcgtttca atagcttttg tcatcgactg cattttcacc
    28381 ggtgtcattc gtaaagtctt ttccccggtg aacgacgtag tcataaacgc tttggctatc
    28441 gtgctcgtat ttaattcagc atttgatgta atcaaagagg tggcagcatg aaggccatcc
    28501 cattcgcgct gttgttcctt tcttcgatcg ttgtggccga caccactgtt tatcagtgtg
    28561 aaatgtctgt agccgacgtt aagaatggcg ctcttaccga cgtcataaaa gcaccatatg
    28621 gagcgatggt cgtagacagc ggcgaccagt tctatgttgt gcgtgacgat cgagtgttgt
    28681 catccccata tctcacaaat cgtaatggca aattaaccgg cgtcggagaa gaccacttcg
    28741 tatacaacaa atacaagggc ttctatggtg ttcacgcttc tcagcaaagc taccttttcg
    28801 atgactgcaa ggaggttgga taatggcatt aacactggca ggtctggaaa tcgagaaaac
    28861 aagcggctac tggcgtgcta agggtttcaa gcagcctggc attcttgagc gtctggaacg
    28921 tgaagatggg tatatcgtcc accagcggcg tgaatggcgt atgtacgatc cagaaacagg
    28981 aaaactgact acaaaagccg gaacactttg gggtctgtta aagaaaatac actaaatgca
    29041 aactgactgc ggtacgttcc gcagtcatat ttcatagtcg tcaccgctga cagcatacac
    29101 aatcaactac cgctgatagc atatcgagag tctatctcac cgctcacagc atactttact
    29161 cgatttttta ccgctgacag catacttaag acattgcatg aataatgtgt accggtatgg
    29221 gtataaccag aacaaaatta ccgctggcag catacgaagg tctgacatat accattaatt
    29281 accgctgata gcatatccaa acaaaaattc ctcaataaaa caccgctgac agcatacgtt
    29341 ctatcaggga gcagcaggca ataaatgcct ttcactacaa gcaatcagcg caatagcaat
    29401 agaatgttag tgagcgcaaa cctatatgga atgcactctt cgaggttagt aaccactggg
    29461 gaggtatgac agagcattga gtggtgatag atgatttacc gcccacagca tacgttcatc
    29521 tcactatacc gctggtagca tatatttaac cgtttacagc atacttttca gaaaaatagc
    29581 cgctgatagc atacatttca ccgctgacag catatcaaag cagtttgaga ctattggaaa
    29641 ggatctcaat catcttgata ttttcaggcg tcaaattctg cgaaagttcg gttatcttgt
    29701 tgataatgtt ctgtttggca tcaatttccc cagctttctc atctagtttt ttgggttcga
    29761 tgtcttcagg ttttggcggt gcgactttga gttttggatt gcggctgtga atctggatat
    29821 agatcgaccg ccctcgctta atctcgctgt attcgagata acccaaatct tggagagctt
    29881 ttaagccgtt gcgtatagtc tgattctgcg agctgacatt cctgctactc aaattgagtc
    29941 gcgcacgcaa tcgagcaagc gataccggcg caggcttggt tggaagactt tcgatgaagg
    30001 tgtacagagc ctgtgctgtt tctttgcgtg gtagcttatt gataaccttt aactgcaaaa
    30061 gaaccttatg gtcaaagcga tatagttcgg ccagcttcgg ttctgcatag aacaccaccg
    30121 tatctttctg ctcgttgtag tccacgctat tgatgaggtg caccatcaga agcgagatct
    30181 tgttagagcc gtcgacgttc ttttcttcat acgttctctg gaaagacaga gttgtacgca
    30241 tgatcttcaa aagactgttt gtaagccggt cgcggagtgt tttgcggatc tgtgacgatg
    30301 gatagccaca aaacttcgca aatttcgtga tgcttaactc gacacgaccg ttaggttcgc
    30361 cgtattctgc cagcgaacgc acaacgccca cccacgtttt gaaatcatga tccatgtcga
    30421 gacgaggacc ggttatcttg atatcggaat agccttcaga acgggctact tcgagctgaa
    30481 caagctcctt tgaagcatcg atctcatttg gcttgttacg cttgctgtat tttgtcccct
    30541 tgagcgtggg cacgaacaat cccagccgca tcaacgcaat tggttggact gtattgttgc
    30601 tattagggac aagttcccct gtgtacaatt caagggaacc ttcttcaaag ttgtcgagat
    30661 tatcttctac ttctttgtta tttttacctt ttttattttt tgtggacatg tggacacctt
    30721 tgtcattcaa ccgctgacag catacttgat ttgccgctga cagaatacca aaaacagttg
    30781 gcagcatacg gtgaaccgct gacagactat caattaccgc tgacagcata catgaacatg
    30841 gcttcagacc agtcgtggcg cggcttacag cgatcgggga tcttatttga tctatacaag
    30901 gatctatcta tggatctctt tattaggatc tatcctgtgg atatgtgaat aattaaaaca
    30961 ggcatttact accttcggcg cacctggtgg gttatcgttg cctcggctaa caatcacaga
    31021 aaaatgacat atggatctaa aacgcacgcg ctgggttcgt cgtcttgaag acggctccta
    31081 cactatcgaa tcaaattcca acctgaataa gcagaagttg ctttgtgaca tctgcggtat
    31141 agcggcgaag tgcccgatct acgaaaccag aattaaactt gataaggctg gtgtgaattt
    31201 tcatttaaac agttgcatca ggtacgttcc attactcgca tttcgtaaac cgatcatcgg
    31261 attggatgct ccctacttca acacactccg ttcaggtgtg acgtggcgag atcgtttatc
    31321 accagacaag ctgatttgcc tcgtatccgc agacacaggg aaaatcatcc gttttgggaa
    31381 agtagacaag gtttactcag gcccagtaga cgaaatgttg cggaagcaca gccggtttaa
    31441 tcatctctgt atgggtggtg agaaaatcga gaaagtagaa gaagtgatcc gcaaatccta
    31501 cggacacttt ctgaccaaag atagcctgct caccgcaatc tacatcagac atgtaaaacg
    31561 tgagttcgac ctcgaatacc acagcgaaga agagcttaac cttgttgacc cacgtccaaa
    31621 agctggcgtc ataagcataa acgcagcgcg taaaaagccc actgacgcgc tgtaaccctc
    31681 cagatcgtat attggcgtag atagaatcta cgccccctca aaatagctct catagcgttc
    31741 tacagtgatc ctgtcttatt tttagtcata cagacaagca aagttgcgcc acgataaata
    31801 ggtatatact tacttataaa ttttgtatat taaggcgctc gtttcattcc taacataccg
    31861 ttatgcatag ttgtttacct cctcattgct cttagaattt gtatcaaaat aaccacaaag
    31921 gaaaaataca tgactttgcc atacggcgtc atttctgact gccactacca caaatgggat
    31981 gcgttctcca cgacgaacgc tgaggggctt aactccagac ttgaaataca gttggaagca
    32041 acgaaagaag cagccatcgc catgaagaag gccggttgta agtacatgtt ggttgccggt
    32101 gatacatttc acgtccgagg aactgtgtcc ccttctgttt tgcattacgt aactgaaacg
    32161 tacaagtgga ttatcaacga gcttgatctg acagtagtaa tgctggccgg taatcacgat
    32221 cttgaaacca acgattcagt atatagcgcc aacgcagcag catcgctgag ttctatcggc
    32281 gtggtaatcg tatgtggcaa gcgcccacac tcaataaaaa ttggtgatgt gactgtccac
    32341 ctgattagct ggcgtaacaa tcatgcggag cttatcagcg atctgaaagc attacgtaag
    32401 agcgtagaag gtgataatca tgacgttgtt atccatacat ccattaacaa agccattcca
    32461 acaatgcctg acgtcggtat cgatgcgcag gagttaaagg atatcggctt tcgtctcgtg
    32521 cttagtgggc attaccacaa ccacaaagag gtcattcctg gagttatcag tgtcggtgca
    32581 ctgactcatc aaaattgggg agatgttgga tctctggctg gctacatgat cgtaaacccg
    32641 gacggcagtt tcagtcacta cgaaaccagt gcgcctaaat tcattaacct ggaagatgat
    32701 gttgctgatg accaaattcg cggcaactac gtgcgtttcc gcgccgtaat tgagaacgat
    32761 gaagaaggca ttaagtacca gaacatcctc aaaacaatgg gtgcaaaagg tgtcgtgtgc
    32821 aacttcatcc gtaagtcatc aatgatggaa gggacagcca gcacaactga aaccagcaaa
    32881 atcgatagcc tgggagagtc ggtatctgct tattgcaaga ttgtccacga tactgacggc
    32941 ggatttgatc tgagcaaatt ggatattttg tgtcaggaaa tcctcaccga agcggagagt
    33001 tcggaggctg tgtgaagcaa agtcgttatg ggagctttcg agactttgcc atcacgatga
    33061 aaagacttga acgaggccag acggtgatgt ttcacaagcc ctacccgcca caaggaaatc
    33121 ccgtagcgtt ttatcttgga aggttaacaa gaaaaggcgt attgaggcgc agatccttcc
    33181 cggcgcatac ggagttcaga ttgaaagaag gccaaaagct aacacacggt atcagaggtg
    33241 ttatatgaag tttttaaagc tccaggttga gaattttatg gctatcgcca gcgcggaggt
    33301 cgagttagat cagcgtggtt tagtgctcat tcagggtgtt aatagtgatg atagttccgc
    33361 atcaagtaat ggctctggaa agtcaactct aatgaatagc ctgatgtggt gtctttatgg
    33421 cgaaacagct catggtgtga agggtgacga tgtgttgtct accgaccatg aaaagaactg
    33481 tcgtgttgca gtaaccatcg aggatgaagg caagagatac gcaatcattc gtcaccgtaa
    33541 acacaaagag ttcaaaaatc gtcttatcgt tcgtggtgaa gatggcgata tgacgaaagg
    33601 caaagatgcg ctgacgcagg agttcgtcga gcgtctgatc ggtgcatcta aagaggtttt
    33661 catggcttcc atctatgcga gccaagaagc tatgccagat ttacctggaa tgtccgacaa
    33721 aaacctcaaa accatcgtag aagaagccgc tggcgttgac agactgacac gcgcctacgc
    33781 tattgctcgt gagcgagcta atgcagctgc cgcacgtatg gatgtggtta aaaccaaatt
    33841 ggagtcgaca atctcgacca ttgaggcaac acagtcagaa attgagtcag cgaaagcctc
    33901 ctctgaatca tgggagcaag agcgttttaa acgttatgac gatgccctgg ctgggctggc
    33961 cagtgccgaa gttgagttaa cggaagttga acttgagatc cgcactctgc ccgaacagat
    34021 acgtgatacc gagaaggcaa tcgaaagtga gcgcaaaaag ttagcctcaa aagaagaaca
    34081 tgacgccaag ttgctcaaag ttcgtggtgc gataactgat attcgggcaa gcatcaaagc
    34141 tacagaaaat agtcaggctg atgcaatgaa ccgcgcgcgt aattttaaga ccaaagcaga
    34201 agaggttggt actaaagtgg gatcaccatg ccctacttgt ggcaaagcct actgcgaaga
    34261 agatctatca acggtgaagg agaatttcat tgaacaagca cgacaggaaa ttggtcaggc
    34321 gaagacactt gcagaggcaa tggctaaaca caaaacgaat cttgagaaag cgttaagcat
    34381 tgagtctgcc cttgttaaaa cgacacctga tgtaacggct atcattgccc ggattgaaga
    34441 gcttacgaaa caactctcat ctttgcgtca tcgtgagaag gaggttgttg ctattgagtc
    34501 tcttgtgact cgtgctcgta ctgaggtcga tcgtatatca aaagagatta atccgtttat
    34561 tgctcttatc gccagacacg aaaataactt ggtatccagt aagtctacct tcaagtcctt
    34621 aaaagatgag ttgaaggcta ttcaggaaca aacgttgcta ttggaaaaag ctcgtcaggt
    34681 ctactctcct gccggggtgc gttctcatat tttgacgtct gttacgcctt tcctgaatac
    34741 acgcactgcc gagtatctca atacgttgtc tgacgggaat attactgctg agtggtcgac
    34801 gatggatgtc actaaaaaag gtgagtatcg cgacaaattc aacattagtg tgcagaagaa
    34861 aggttcaagt aagtcgttcc aaaccctctc tggtggtgag aagcggaagg ttcgcattgc
    34921 gtgttctttg gcattgcagg atctggttag taaccgggcg agtaaaaaca tcgatttgtt
    34981 tatcggcgac gaaattgacg atgcactcga tacagccggt cttgaacgcc tcatgggtat
    35041 tctggagtcc aaagctcgcg agcgaggtac tgtgctgatt atctcccata aagagatgaa
    35101 gtcatggttc cgggaaacta ttacgctgga agttaaagag gggcgcagct atgtcgttta
    35161 aattaagccg ctcgcagttt ttgcaggtat ttgcagtgat gcagtcgata aaactgatca
    35221 atgggcatac ttccaatggt gcggctccac gtattctgtg gggcagcaac aatattgacg
    35281 gagtacaatt cgccgcgttg cttggtctaa tatccgagac accattgatg caaagtttga
    35341 aatcactacc acctggatgt attgcgccga tcctgattaa tccttttgtt gaggggggat
    35401 atcttcccaa cgtcgggcct gggtttattg catcccatga aactgaagat cttaacatta
    35461 atagcgaagg gttctttggg ggaatgggtg cgcatcactg tatggctttc acgaacctta
    35521 ttcgacttgc caataagcgg gtggatagtt tggcatcgcc aggtgatgct tttactggtt
    35581 tccttatcca aaggagggat aaaaagtaca gtgcggacaa actacagttt gttggtaagt
    35641 atggagaaat ggtagaaatc gaacttcagc tccctcatgt tttagcaaac gatagtgcag
    35701 acagtcggag gctgttgggc atcatgcgtc atttcatagc aagtggcgtt aaacatgccg
    35761 tagataaacg tgtcacgcag gaaaatgagt attcagactt tgcaaactat ccccaaccaa
    35821 cgttgcaaac ggcaatagta accaattcgt tggaggcgag attattggaa aaccctatat
    35881 ggggaacatg gtaaggagac tatatgagta aaaaaatcag cgtagttggt gttgatccct
    35941 ctatgagcaa ctttgggctt gctgtgggca ctttagacct tgaaacggac gaacttgaga
    36001 ttcacggcct tactcttgtt gagactaaag cggggagtaa caaaaagacc gttcgtgtga
    36061 acagtgacga tctgcgccgt gccagtgaaa tatggcgtgt tgcgaagcca atcattgata
    36121 aggcgaatat ggttttttgt gaactaccgg ttgggagcca aaactctcgt tcgcagacgt
    36181 cttacggtat ttgtatcggt gtacttgcgt gtgtggataa gccattgatc caggtcactc
    36241 caaacgaaat caagcatttt gttggcaata aacttactac atcgaaagaa gagattatcc
    36301 agtgggctac gaaaaaacac cctaaagcac cgtggctgcg tcgtaagcaa tctggacagg
    36361 atgttctcgt gaacaaaaac gaacatttgg ctgatgcggt ggctgccatc cataccggta
    36421 tgcaaacgga tcagttccgc caggttcgcg atgttcttaa gtctctcatt tgatttcatt
    36481 gataggtaag tgcttatcta ttaacatgag ccactatatt tagtggccct ctttatttgg
    36541 tgatacatga taagcatcgt aaaacgtaac ggccaaacag agccgttatc cgaagaaaaa
    36601 tacaaccgcg tcgtaatgta tggcgtagaa ggcattcgtg gcgtaagcgc atccgctgta
    36661 gcaatgggag ctgcggccag catttttgat gggatgacca ccagccagtt gcatgaggct
    36721 ttggttaaat ctgccgctga tttgatctca ccagaagcac caaattactc acaggtggct
    36781 gcccgcctga acatttttaa aatccgcaaa gatgccttcg gtcgttacga ctatccgaac
    36841 ttctaccaac acattgtcaa gaacgttaac aagggcgttt atgacaagga tttgctgaca
    36901 cattattcgt ttgaagagat cgaagaactc ggcaattaca ttaagccgaa acgtgacgat
    36961 ctttttggct atgcagctac ggtgcagttg caaagcaaat acctcgttca aaaccgtgtt
    37021 actggtgaga ttcatgaggg gccgcaacat atctatatgc tggtaggcat gtgtctgttc
    37081 cagaattggg aagacgactg cgctggcaaa acacgtatgg agatggtcaa aggtttctat
    37141 gacgttacaa gtacgttcaa actgtctctg cccacaccaa tcatggccgg cgtccgtact
    37201 ccaacccgtc agttctccag ttgtgtgctg attgagtctg gcgatagtct gaaagggatt
    37261 agtgcagctt cagccgcaat tatcgactac gtttcacgtc gtgctggaat tggtattggt
    37321 tttggccgta tccgtgcgct gggcagcgag atccgcaatg gtgaagccac ccataccgga
    37381 gttattccat tcctgaagca tttccagacg gctgttaaat cttgttcgca aggtggtgtt
    37441 cgtggtggcg cagcaacagc gttttacccg atctggcatc ttgaagttga aagtctgctg
    37501 gtggtgaaaa ataaccgtgg tattgatgaa aaccgcgttc gccatcttga ttacggcgtc
    37561 atgagtaacc gtctaatgta ccgtcgactc gtcagaagcg agaacatcac tctgttcagc
    37621 ccgcatgatg tgcctgatat gtacgaagcc ttcttcacag accaggagct gtttgaaaag
    37681 ctgtaccata aatacgaagc cgatgattca attcgcaaga agtcagtacc tgccattgag
    37741 ctgttctcat ctctgatgca ggaacgagcg tccacgggcc gaatttatat tgcgaacgta
    37801 gatcatatta acgagcatgg cgctttcatt cctgctcttg cacctgttcg ccagtcaaac
    37861 ctgtgcatgg agatcactct acccactcgt ccactggcat ttaccgacga cccgaacggt
    37921 gagatcgcgc tatgcacttt atccgctttt aacctcggag ccatccgttc actggagtct
    37981 cttaaagagg tggcgttcta tgccgttgct gcactggatt cgttactgga ttatcaagac
    38041 tatccgatgg aggcagccga agtgcctgcc aaagctcgtc gtagcttggg aattggtgta
    38101 accaactttg cttattacct ggcaaagaat ggcgttcgtt attctgatac cgctggcaat
    38161 aaactggtgc atgaaacgtt cgaagctatc cagtattacc ttcttgatgc cagctgccga
    38221 cttgctgaag caaaaggtga gtgtgactgg tttgagcaga ccaagtacgc aattggtcag
    38281 ttgccgatcg accattaccg ttcttcatta gacgaaagtg gcgaaaccaa ctttgagtta
    38341 aagatgccgt gggaagaact gcgtgaacgt attgcaaaat acggccttcg caactccaca
    38401 ctgacggcac aaatgccatg cgagacttcc agccagatca ctaattccac caacggcatc
    38461 gaaccgcctc gtggcccggt gtcggtgaaa tcttctaagg acggcatcgt taagatggtc
    38521 gtgcctgagt ttgaaaaact gaaggaacag tatgaatacc tgtgggatat gccggacaac
    38581 cgcggctatc tgacaaaggt ggcgatcatc cagaagttct ttgaccaggc tatttcagcc
    38641 aataccaact atgacccttc tcgctttgaa ggcgataaag tcccaatgat gacgctactg
    38701 tcagatttgc ttctcgccta caagatggga gttaaaacgc tttactacca caacaccaga
    38761 gatggggcag gaaagcgtga tgacgacgaa ccgcagaatc cactgacgca agctgtagcc
    38821 gtcgagccag aagatgagtg cgacggagcc tgcaaaatct gacatatggt ggggcatatc
    38881 cccaccttat ctttgatttg taagccttgt ttaaacacat aagataacaa cttgtttaaa
    38941 cacatcaaaa aaggaaaaac acatgtcata ttcaacgttc cgtttgggtg ctaatgatgc
    39001 aaccaaagag cctatgttcc tcggacaatc tgtcaacgtg gcacgttacg atcagcaaaa
    39061 ataccgtgat tttgaaaagt tgattgaacg tcaattgtct ttcttctggc ggccggaaga
    39121 agttgatatt tcgagcgatc gtatcgactt caacacgaag ctgcgtgacc acgaacgtca
    39181 catttttctg agcaatctcc gttatcaaac gttactcgac tcagttcagg gacgtagccc
    39241 aaatgcaacg ctgctgccgc ttatctctat tcctgaactg gaaacgtggg ttgaaacatg
    39301 gtctttctct gagactatcc atagccgcag ctacacccac attattcgtg gcatggtgga
    39361 cgatccgagc attgtttttg acggtattgt tacggatgaa gaaatcatca accgagcgat
    39421 cagtatctct gctgaatatg acaggcttta tgggatgacc tgcgagcgcc agtcgttagg
    39481 tgagaaagag tttgaacgtc tgtacgtaaa tgaatatggc tgggagccat accctttgca
    39541 ccgtcagctt ttccgcacgt tggtgtccat taatgcgctt gaggcgatcc gtttctatgt
    39601 aagttttgca tgtacgtttg cctttggcga acggaagttg cttgagggta acaccaaaat
    39661 tatgcgcttt attgcccgtg atgaagctct gcattgcgaa ggaactgaac gcatgatccg
    39721 cttcatgcgt accggtcgcg aaggtttatt gtggaaagag attgctgctg atgaagaaaa
    39781 cgtcatttac gacaccatga aatcagtcgc cgaacaagaa atgaactggg cagactatct
    39841 cttcaaagac ggttcgatga ttggtttaaa cgcggatatt ctgaagacct atgtaaaata
    39901 ccgaaccaat ctggctatga atcgtcttgg cctgaaggct ttatttccag aagttaccac
    39961 agatccgctg gtctggatga acaagtggtt gttaaccgac acactgcaaa ttgcaccaca
    40021 agaggcagag caaagcacat atctggtagg tcagatcgat tctaccgtgg acaaggcttc
    40081 tctaagccag tttgcagacc tgtaaaccga tacaaagcat tatgtggcct ggcaacgctg
    40141 ggccacaatg gatcacaaga attaagaagg aacaaaacta gcatgaactt taccaaactg
    40201 actgaccacc tgaaacttgc caccgatcgt ctcattggat ttaagccaga accatatgag
    40261 ttgcatgaag gtcatggtgt agctactgaa agtatttaca agatggtcga tcagtttcat
    40321 gaactcttcc agcatccgag acgcgttatg ccgacaccag agctgcttcg tctccgtgca
    40381 agcctgattc atgaagaagc tgtagtggaa ggtattccag ctgcaatgaa tggggatatt
    40441 gagcaactgc tggatgcaat ggccgacttt ttatacgttg gtgttggtac gatggtcgcc
    40501 atcaaaggtg gtatttctac cggcatgacc tattacacgc aggaacagag cattgatcgc
    40561 tttatgcaga caatttttgt gcccggtaac actgttttcg atgatatggc aatgccattt
    40621 caggaagctc gtgaggcgtc atgtatgctc gaagagctgg cagataaact tgagaacaag
    40681 actgttaagg attctgagct gattcaggaa ctgcgccgtg tcatgaacaa aatctatgtg
    40741 gcgtgcatga tgacctatcg actggctgat ttcctcggta tcaatgtagt cgagctggtt
    40801 ggcgaaattc atcggtctaa catgacaaaa ttatggcctg ctgatgtaga ggaacgtcgc
    40861 caggctgtgg ccaactgcaa atacgactct tcagacctgg gatttcgcca tgctgatggt
    40921 accgataaga tgatcggttt tcgaatttcc gatggaaaga ttctgaagtc tccaacctat
    40981 agtgatgtcg atttatcctc ctttgttgag caagctaaag cctcagcaat gtacggaatg
    41041 atcaaaaaat aattgtaggt agttatctat ctatgtatat tgtgttggcg cgttaaattc
    41101 ctgaaacaac tattcgtttt tggtggccta tggccaccat ttttttatct atctggcctt
    41161 gttccctcaa taaatgtaaa ctcacgtaat gaataagtgg ttacttatct ttgtgaggtt
    41221 tttgtgtcac tccttttgaa tcgtgagcat acgaacggtc aggtaacaaa cgcatcgtat
    41281 gcaaaagtta ttgagacggt gcttaaaagc ggcgtgcagg ctgatgatcg cacaggcact
    41341 ggtactttaa gcacctgcta cgttccctct tactacatgc ttactggtgg gactgtgccg
    41401 cttatttctg gaaaggcggt aaatcttaag ccactgcttg tcgaacttga gtggtatctg
    41461 aaaggcacgg gcaacatcca atttctcaag gataacggcg ttaagatttg ggatgcatgg
    41521 gccgatgaga atggcgattt ggggccggtt tacggtaagc agtggcgtcg atgggaagat
    41581 acccgcatcg tgagccatag tgaatatctg agcaagatcg atactttccg tgaacgcggg
    41641 tacaaagtcg agggatacct gggtatcagt gaagatcgcg tagtgctgtc ccgtgaaatc
    41701 gatcagctac agcgtattgt cgatacactg cgcacgaacc ctaccgatcg tcgcatcatg
    41761 cttaacgcat ggaacgtagg cgagcttgag gatatgaaac tgccaccttg ccactttgtc
    41821 ttctctttgt ggagtcgtga gctggatttt gaaacccgtt taacgatggc aactgacatt
    41881 ggtcttcaac acagtcgcct cggttacgag tctatctaca ccaagatgct atacgatctg
    41941 gagatggacg gcagtgttac tgaagctgaa ctggatgaac ttggaatccc caaacgcatc
    42001 ctcaactcct gcctcgtaca gcgtagcgta gacacttttg ttggtatgcc attcaatatt
    42061 gctggctatg gcattctcac tcattttctc gcgaagatta cgggtcacat ggccggtgca
    42121 tttgtgcatt ttggctttga cgtgcatttg tacaacaacc acatggaagg tgtgtgtgag
    42181 ctaatgaaac gacaagctcc agagcattca gatccggtcg ttattttccc tcatgaatgg
    42241 tcagagttgg atgatttcaa atgggacgag attttaattc ttggctatga ccctctaccg
    42301 tggatcaagg ttccagtggc ggtgtgatat ggcaagaggt atgtatgtct tatgcgaaat
    42361 tgaaggtgtg ctggcaaata ccagtcatcg taaatctgta tctgacgcgg atgcaggcca
    42421 gctcattgcc ggtgatgaac tcattttccc caccagccgt atgttgcgtg gttttgctcg
    42481 ctcaggggct gaagtggtgc ttatcagtag ccgctctgaa actcttgaag cgcccactaa
    42541 acgatggctg aaagattttg gcgttgatta cgactggctt catctcgtac cgaatagcac
    42601 cagttatgag aagcatatta aacgcacatt agcggagcat aaaggcgatc tgcttatcgc
    42661 tgcgctggtg cacgatcctc gactccgtgc cgctttagct gactctcatc atagaccggt
    42721 catctatgag gtgagcaaat gaagatgata gctgctgttg gccgtaacta tgagatcggc
    42781 atagcgaacg aactgccctg gcgttgttct accgatctga agctatttaa gagactcacc
    42841 aaaaacgcca ctgtcgttat ggggcgtaaa acgatggaaa gtctcaaacg ccctcttcca
    42901 gagcgtcata acctcgtttt gacgcgctct catggctttg taccaaatgg attctaccct
    42961 gctggtgtgg atgatgtgtt gcgattacca gagcctgtgt gggtgattgg cggggaacaa
    43021 atttactcgc tattcatgcc gcatgttgaa gagatttggc tctcccacat cggcgttgat
    43081 gtgccaaacg ccgatgcatt cttcccggca agcatgatgc gtaatttagg ctttgtgcct
    43141 gttgaaacag tttttaccca acgagccagc gaggaagagc ctggcttttc gcagatcgta
    43201 tacagaaggt cgtaatggat taccggattg ggatcactgg tgctcagggc agtgggaaaa
    43261 caaccctggc aaaatatatc gacaaacatt acggaatccc ttacgtggat gctggggtcg
    43321 gaagtttgat gagccgactc ggtgttcgag taggtgagtc tatgcctcta tatgagcggc
    43381 ttcagattca aatggaaata gcaaagcata tagagctact tacgcgtggt gttgaaggct
    43441 ttgttatcga tcgcacacct gctgatgtta tggcctacac gttggatttg gtcggccata
    43501 ccaatgaaga tcgatgtatt gagttagccc tcgatatcga aaagttttgc cacaaaactg
    43561 ctatttcaaa ctttaacgcc attgctggcc tacgcccggg ggtcgctctc tcagagcgag
    43621 attacttgcg gtcacaacga gcatcattag accgtctgta tgtcgctcgt attgatgcgt
    43681 tgatgtgcgg ggaactgaca aaaattcacc tgcatccgca aaggggagat ctgcaaacct
    43741 tcgtcgtttc caaccggtat cgcacagttg aagcaagagc cagatcagta atgagaatgc
    43801 tcgataacgc ggtagaaaag atagaaaacc gcttctgtgg ccgagtaacc gttcattaga
    43861 aattgttcgc ctcttcgaca ttgcgacaat aaaactctca aaatgggtta aggataaaaa
    43921 atgtttagtg aaatgttgct tgaagatgaa ctggatcgga aaacaacaga ggctttgatt
    43981 cgtgtagcgg acgaacattc ccggtcgctt atgagcgatc gagaggctcg tctggctatt
    44041 cgtgccatat tcgaaactgc gcaggggctt gttggcgcac aagtaggtga agccattaac
    44101 atcgccatgt ctcagttcag tgaagacagt aaaaagcctc tgtttcctat gcatttgatg
    44161 ctggctggtg gcacggtgct ttatatctct gtttgtctgg atagcaacca aatcaatatt
    44221 ctcaacactg cgtcaggtaa gtggaaagat ccgattgtct gtgaaaccag tgaagaaact
    44281 ttgaaaaaag cggctcaatt tgtacgtagc gcactactta agggcgctaa gaagttgtaa
    44341 ggagttctga tgacaacgat tgttgcaggc atcgatatcg agtctacggg actggatttc
    44401 cttgctggtc ataaaattat tgaaatcgca attacccgct atgaactgga gacacagaga
    44461 catattgata gtctggagat gcgttttaac cctcgcagaa acatagatcc gaaagctcaa
    44521 gccgttcatg gcatttcatt agaacagctc gcagctgaac ctttgttgtc aaatcatgcc
    44581 agcgaaattg gcgcttatat gggggcatgt agtgcgtgga ttgctcataa cggcgaagcg
    44641 tttgatatac catttattcg acacgagttt tcagggtatg gagtaagact gccagacgtt
    44701 cccgttatag atactatgtt atcgggattg tgggccacag aagacggtaa acgtcctcgc
    44761 cttgaagaat tggccttctc tcttggcttt atatacgatc atgccaaagc acatagtgcc
    44821 ttatatgaca caaacttaat gatgcaatgc ttctttaagg cacgtaataa atacggattt
    44881 tttaaattac cctctgaaat tgtgtaaaac aaaagcctgc tttaaagaga gtttgaagca
    44941 ggctttcttt taaagaacag tcgcctttca atcatttcct gcctgtattt aatacttttc
    45001 cgcctgatag gtttagtcaa aatgtagcca tcgaaacgca aatgcaacta aacagaagga
    45061 gacttacatg agttcggttg aaaatgtaat gacaaatgat gatctggatg agctgacagc
    45121 catgttgcaa tcacttgatg aaccagtaaa aaaagctgca caggttgaaa atactgatga
    45181 tattgacgat ctgcttctcg gcctcgatgc tggcgtagcc atgagttctg atgatgttgc
    45241 cgaagaactg ttcaatgaag aaaaagcagg tgatttcagc tccgctttaa atgagttgga
    45301 gttagctcat gagcctataa acgtaattaa ggctgaaagt gttgaatctg ccgaaaacga
    45361 gccagaacaa ttgggattta ttgaggttga agagtgtgtt gaggttaatg atgaattaaa
    45421 agttcaacag tcaaatgata gcaatacaaa taaaatagct cgtactgcaa gaggtcctcg
    45481 ttttacttta agtgataaag atgatttgtt tttcaataaa gcaggcttag aaaaagatat
    45541 tttcttagac gcttacgaaa acgcgcctgt caaagcaaag gataagatat taaaccttct
    45601 taattggttt agcggaggtc cagatattag tgtttacacg gtaatttcca tgagacacct
    45661 tctcacagaa aagaaggcta caagtaatag tattaagatt gctttaatga gcaatccaga
    45721 aaaaccgtat ccgcttaaca ctgcgtcaac tcaggctggg caaatgatgg ctgtatttcc
    45781 agctacagga attgctgtta gagacggtgg aaatctaaca ttgaacgaag aatcaccgat
    45841 cgttaagaag tttgtcgcgg agtacactat tggatgacgt tcccctactg aaaataaagc
    45901 ccatagagag ctttatagtg ctgggtaagc taacccatac ccagcaccac aaaaacgcgc
    45961 cagagagctt cttgttcgca tttctggcgc gttttatttt attgccatac ataaaatcaa
    46021 atgcaaaaat aggtaatcac ttacttaccg agaaagaaga tgattgcagc cgaaaaaatc
    46081 aaacagcgaa agcgcgacaa ctctcttcgt gacctctgga gaacacctga ttggctgttt
    46141 tctgccattc aacgttatct tggagtgaca tttgatgttg acgttgcctg caacaaggac
    46201 aatgcaaagc tgcctatttt cataggcgtt gagcgcgatg ctttgaaatc tgaatggggg
    46261 cagccaggta caattgcctt cctcaatcca ccctactcca aaatctcccc ctggattgat
    46321 gcggctatac gtgagcaggc tcgcggagtt acaacagtga tgctaattcc tcaatccctc
    46381 gatacaaagt ggtatgagcg tgcaacagag tgtgcgaatg agacgattat tctgtctggt
    46441 ggccgcgtag cgtttgtcga gcctgacgtc aatttgggtc aggtagaagt aaacatcaac
    46501 cccggtggca gtatgctcgt tgtttttcga ggattctgtc aggacgctgg gcactctata
    46561 agcaagatcc ctttggacgt catgaaaagt ctgggagggt atgatcctgc gaatgtgatc
    46621 aggaaaaaaa gaccatcaaa gaaggctgct tagtttgttc tggcgtctgt aattagcctg
    46681 cttctgtata taaataacta catattaatt attaatatac ggaagcaggc tgttttatat
    46741 cagagactcc cagacctgaa caccactaca gaatccacta gaaccccttc ccagacgctt
    46801 taaaatcgat tttatgaacc actttaagga aaccaacatg tcatacccga ctaatgtcgt
    46861 tgcgctcgta gagagcgatt ttctggccca ggctcgtgaa atgatgaaag atcgtgagca
    46921 ggctttcaac ttgtacgagt gggcaattaa gtgcttgcat cttggcgagc atcgcgaact
    46981 tgttgaacag cttttaggtg agttgatcaa tgaggtgttt gccttgaatg ttcaactaca
    47041 tggtcgagaa aataatcaat caaaatgata gataagtaca aactattcat aaagtgaatt
    47101 gtaagtgcta agatctgata gtttccagtc gtagactgga ggctcgacct gatgggtggg
    47161 ggtaagcgtc actggcgtca ggtttaaaaa agctcactac cagcgtagaa ccggtgccgt
    47221 ataggtgtcg gggaaggggg aaccaaagtg agcggagaca agggtcactt tatgattgtc
    47281 gagtctgggg tgtttcgaga ggttgaatcc agtactcccc ttcataaagt gtgggaagat
    47341 ctcggttctg gggtgctgtc atccataact tcccaagtct aagctggcag tagacttaga
    47401 ccatagcttt tcaggttatg aaacgaccag gttggtgagg aattttatac tcacctccct
    47461 gggagagtat tacctgaaaa gacaacctct cacttcgttc gaggtgaact tcactcactt
    47521 cgttcgttca gttcaggttt ataaaaacct gttctgggaa gtaatttgtt tattttaata
    47581 attattaaca cgcacgcgtg tgcgcacgcg cgaggaaaaa aatcggcgcg gcgcttgatt
    47641 caggagttta tatgacgacg aagacaccag cccgatcgca agcaaaaact cgcaaaaaag
    47701 ccaaaaacaa aaattctccc cgcaccaatc ccacaacgcc tgtcgtagag ttcaatcccc
    47761 agcttaaaac cgtgaaaatc ttcagtgatg gctcttgcct taaaaatccg ggtggcccgg
    47821 gcggttacgg tatcgttctt cagtatcgtg gagaggaacg cgagttctca gatggttttc
    47881 atagcaccac caataaccgc atggagatga tgggggcact tatcgggctg gagcgtttga
    47941 aatatccatg caacgttatt ttgcactctg atagccagta tctgaaaaac ggcatgacac
    48001 agtggatgaa atggtggaaa cgcaatggat ggatgacttc tgacaaaaaa ccggtaaaga
    48061 atgttgatct gtggaagcgt ctggatgagg ccgcaagtcg acataatgtt cgctggaagt
    48121 gggttaaagg tcacgccggg catcgtgaaa atgaaatatg tgatcgactc gcgaagatcg
    48181 cagctttttc agcagcagat atgcctcaca agaaagatat tggttttgtt tataacaagt
    48241 agtaagtaag tgtttaccta tcattttaaa tcatgtatct tatcggcgtc aggatgacaa
    48301 tgtgtcggta agacacagt
//
LOCUS       DAZFZC010000028        48294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFZC010000028 DAZFZC010000000
VERSION     DAZFZC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.028"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            172..1389
                     /gene="alaA"
                     /locus_tag="ACHKXP_001839"
     CDS             172..1389
                     /gene="alaA"
                     /locus_tag="ACHKXP_001839"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO7646860.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1473..2072
                     /gene="yfbR"
                     /locus_tag="ACHKXP_001840"
     CDS             1473..2072
                     /gene="yfbR"
                     /locus_tag="ACHKXP_001840"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7646861.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEIFVPSFHLSLDEISQDSPL"
     gene            complement(2131..3963)
                     /gene="yfbS"
                     /locus_tag="ACHKXP_001841"
     CDS             complement(2131..3963)
                     /gene="yfbS"
                     /locus_tag="ACHKXP_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO7646862.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGMLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4050..4700)
                     /gene="hxpA"
                     /locus_tag="ACHKXP_001842"
     CDS             complement(4050..4700)
                     /gene="hxpA"
                     /locus_tag="ACHKXP_001842"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO7646863.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4711..5205)
                     /gene="yfbU"
                     /locus_tag="ACHKXP_001843"
     CDS             complement(4711..5205)
                     /gene="yfbU"
                     /locus_tag="ACHKXP_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO7646864.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5288..5743)
                     /gene="yfbV"
                     /locus_tag="ACHKXP_001844"
     CDS             complement(5288..5743)
                     /gene="yfbV"
                     /locus_tag="ACHKXP_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO7646865.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6081..7283
                     /gene="ackA"
                     /locus_tag="ACHKXP_001845"
     CDS             6081..7283
                     /gene="ackA"
                     /locus_tag="ACHKXP_001845"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO7646866.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7358..9502
                     /gene="pta"
                     /locus_tag="ACHKXP_001846"
     CDS             7358..9502
                     /gene="pta"
                     /locus_tag="ACHKXP_001846"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7646867.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9692..11212
                     /gene="yfcC"
                     /locus_tag="ACHKXP_001847"
     CDS             9692..11212
                     /gene="yfcC"
                     /locus_tag="ACHKXP_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO7646868.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11245..11787)
                     /gene="yfcD"
                     /locus_tag="ACHKXP_001848"
     CDS             complement(11245..11787)
                     /gene="yfcD"
                     /locus_tag="ACHKXP_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO7646869.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11845..12396)
                     /gene="yfcE"
                     /locus_tag="ACHKXP_001849"
     CDS             complement(11845..12396)
                     /gene="yfcE"
                     /locus_tag="ACHKXP_001849"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO7646870.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12452..13096)
                     /gene="yfcF"
                     /locus_tag="ACHKXP_001850"
     CDS             complement(12452..13096)
                     /gene="yfcF"
                     /locus_tag="ACHKXP_001850"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO7646871.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13232..13879
                     /gene="yfcG"
                     /locus_tag="ACHKXP_001851"
     CDS             13232..13879
                     /gene="yfcG"
                     /locus_tag="ACHKXP_001851"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO7646872.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13936..14298
                     /gene="folX"
                     /locus_tag="ACHKXP_001852"
     CDS             13936..14298
                     /gene="folX"
                     /locus_tag="ACHKXP_001852"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO7646873.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14319..15212
                     /gene="yfcH"
                     /locus_tag="ACHKXP_001853"
     CDS             14319..15212
                     /gene="yfcH"
                     /locus_tag="ACHKXP_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO7646874.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15260..16150)
                     /gene="rpnB"
                     /locus_tag="ACHKXP_001854"
     CDS             complement(15260..16150)
                     /gene="rpnB"
                     /locus_tag="ACHKXP_001854"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO7646875.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16347..17120)
                     /gene="hisP"
                     /locus_tag="ACHKXP_001855"
     CDS             complement(16347..17120)
                     /gene="hisP"
                     /locus_tag="ACHKXP_001855"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO7646876.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQRFLKGSLK"
     gene            complement(17128..17844)
                     /gene="hisM"
                     /locus_tag="ACHKXP_001856"
     CDS             complement(17128..17844)
                     /gene="hisM"
                     /locus_tag="ACHKXP_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7646877.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17841..18527)
                     /gene="hisQ"
                     /locus_tag="ACHKXP_001857"
     CDS             complement(17841..18527)
                     /gene="hisQ"
                     /locus_tag="ACHKXP_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO7646878.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPSMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18617..19399)
                     /gene="hisJ"
                     /locus_tag="ACHKXP_001858"
     CDS             complement(18617..19399)
                     /gene="hisJ"
                     /locus_tag="ACHKXP_001858"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO7646879.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19620..20402)
                     /gene="argT"
                     /locus_tag="ACHKXP_001859"
     CDS             complement(19620..20402)
                     /gene="argT"
                     /locus_tag="ACHKXP_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO7646880.1"
                     /translation="MKKSILALSLLVGLSTAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20668..21237)
                     /gene="ubiX"
                     /locus_tag="ACHKXP_001860"
     CDS             complement(20668..21237)
                     /gene="ubiX"
                     /locus_tag="ACHKXP_001860"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO7646881.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21332..22849)
                     /gene="purF"
                     /locus_tag="ACHKXP_001861"
     CDS             complement(21332..22849)
                     /gene="purF"
                     /locus_tag="ACHKXP_001861"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO7646882.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22886..23374)
                     /gene="cvpA"
                     /locus_tag="ACHKXP_001862"
     CDS             complement(22886..23374)
                     /gene="cvpA"
                     /locus_tag="ACHKXP_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO7646883.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23633..24295)
                     /gene="dedD"
                     /locus_tag="ACHKXP_001863"
     CDS             complement(23633..24295)
                     /gene="dedD"
                     /locus_tag="ACHKXP_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO7646884.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24285..25553)
                     /gene="folC"
                     /locus_tag="ACHKXP_001864"
     CDS             complement(24285..25553)
                     /gene="folC"
                     /locus_tag="ACHKXP_001864"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO7646885.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSLVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25623..26537)
                     /gene="accD"
                     /locus_tag="ACHKXP_001865"
     CDS             complement(25623..26537)
                     /gene="accD"
                     /locus_tag="ACHKXP_001865"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO7646886.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26693..27352)
                     /gene="dedA"
                     /locus_tag="ACHKXP_001866"
     CDS             complement(26693..27352)
                     /gene="dedA"
                     /locus_tag="ACHKXP_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7646887.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27435..28247)
                     /gene="truA"
                     /locus_tag="ACHKXP_001867"
     CDS             complement(27435..28247)
                     /gene="truA"
                     /locus_tag="ACHKXP_001867"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO7646888.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTALRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28247..29260)
                     /gene="usg"
                     /locus_tag="ACHKXP_001868"
     CDS             complement(28247..29260)
                     /gene="usg"
                     /locus_tag="ACHKXP_001868"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7646889.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29326..30462)
                     /gene="pdxB"
                     /locus_tag="ACHKXP_001869"
     CDS             complement(29326..30462)
                     /gene="pdxB"
                     /locus_tag="ACHKXP_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO7646890.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLYDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30561..31556
                     /gene="flk"
                     /locus_tag="ACHKXP_001870"
     CDS             30561..31556
                     /gene="flk"
                     /locus_tag="ACHKXP_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO7646891.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGTGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31553..32731)
                     /gene="yfcJ"
                     /locus_tag="ACHKXP_001871"
     CDS             complement(31553..32731)
                     /gene="yfcJ"
                     /locus_tag="ACHKXP_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7646892.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32996..34216)
                     /gene="fabB"
                     /locus_tag="ACHKXP_001872"
     CDS             complement(32996..34216)
                     /gene="fabB"
                     /locus_tag="ACHKXP_001872"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO7646893.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34375..36381
                     /gene="mnmC"
                     /locus_tag="ACHKXP_001873"
     CDS             34375..36381
                     /gene="mnmC"
                     /locus_tag="ACHKXP_001873"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO7646894.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDAGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36502..36780)
                     /gene="yfcL"
                     /locus_tag="ACHKXP_001874"
     CDS             complement(36502..36780)
                     /gene="yfcL"
                     /locus_tag="ACHKXP_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO7646895.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36814..37362)
                     /gene="epmC"
                     /locus_tag="ACHKXP_001875"
     CDS             complement(36814..37362)
                     /gene="epmC"
                     /locus_tag="ACHKXP_001875"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO7646896.1"
                     /translation="MNSTHHYEQLIEIFNSCFADNFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37362..38171)
                     /gene="yfcA"
                     /locus_tag="ACHKXP_001876"
     CDS             complement(37362..38171)
                     /gene="yfcA"
                     /locus_tag="ACHKXP_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO7646897.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38171..38995)
                     /gene="mepA"
                     /locus_tag="ACHKXP_001877"
     CDS             complement(38171..38995)
                     /gene="mepA"
                     /locus_tag="ACHKXP_001877"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO7646898.1"
                     /translation="MNKTAIALLALLASSACLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38999..40084)
                     /gene="aroC"
                     /locus_tag="ACHKXP_001878"
     CDS             complement(38999..40084)
                     /gene="aroC"
                     /locus_tag="ACHKXP_001878"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO7646899.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40119..41051)
                     /gene="prmB"
                     /locus_tag="ACHKXP_001879"
     CDS             complement(40119..41051)
                     /gene="prmB"
                     /locus_tag="ACHKXP_001879"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7646900.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISRQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41217..41768
                     /gene="smrB"
                     /locus_tag="ACHKXP_001880"
     CDS             41217..41768
                     /gene="smrB"
                     /locus_tag="ACHKXP_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO7646901.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKVSEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41894..42718)
                     /locus_tag="ACHKXP_001881"
     CDS             complement(41894..42718)
                     /locus_tag="ACHKXP_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO7646902.1"
                     /translation="MKTIWILFCLLITWLSSTSVQASVTFGSVDVLYGASGQSDPTME
                     FYFTIISPRNGAYTAKYYDPHAVPRNNDYLVTDQWPGPGAAPIIQIVGFGNAGASQCP
                     GLPSGRNCQYLTFSITIDAADDFGCPWLASVYSVVTDYGASYRAPTANSTVCPTVPVD
                     TFDVSWSENRVNHNLGISLHSTGGFIEKTVSTYLMESNKLCDSSVMDRRGDYCRFVSG
                     LITFSSYGCDNAKVTVTPIEQAVTDKKLHDIVVRVDTSSMQPIDSSCRFQYILNEL"
     gene            complement(42720..43271)
                     /locus_tag="ACHKXP_001882"
     CDS             complement(42720..43271)
                     /locus_tag="ACHKXP_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7646903.1"
                     /translation="MTRNQTYYTRYTAGSLLGLVLLAQGTATSAKNNLHFTGNLIDKS
                     CTLVAPGGLLAEVRFPTISSRDLSTQMQSGKVSMAFELENCKGSSQSQNSVKVTFTGT
                     EDTGQPGFLALDSDSQAQGVGIGIETVDGVPVKINNNSGATFVLSDGSNTLLFNTWVQ
                     AKSGRDVTLGNFTATATATFEYL"
     gene            complement(43268..43747)
                     /locus_tag="ACHKXP_001883"
     CDS             complement(43268..43747)
                     /locus_tag="ACHKXP_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7646904.1"
                     /translation="MKQIAFFIMLVSYATVVSSSPDITFHGNLIAPPTCIVSENKTIE
                     IAFNEVIISDISGDNYKREVPYSVECDSENTDTGIQMKLTWTGVETDFNDSAVETDVV
                     GLGVELQQNGQRFQQGKAINISKTNLPKLHAVLVKKSGAVLSEGVFEAYATLQVDYQ"
     gene            complement(43744..44229)
                     /locus_tag="ACHKXP_001884"
     CDS             complement(43744..44229)
                     /locus_tag="ACHKXP_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7646905.1"
                     /translation="MVGGLLICTTVGELTYAGSKPVNMVLRVQVDGPPPCTVTGSDVD
                     FGDVLIRRIDGSQYLQPVTYKLDCGQRLTGADDLRMQLQGPTSVINGETVLNTGIDGF
                     GIRIQNATNDSLITVGSSAWLPFSIDNQPTLEAVPVKQNGVSLVSSAFSATATMVVDY
                     Q"
     gene            complement(44267..45019)
                     /gene="yfcS"
                     /locus_tag="ACHKXP_001885"
     CDS             complement(44267..45019)
                     /gene="yfcS"
                     /locus_tag="ACHKXP_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7646906.1"
                     /translation="MSDLLCSAKLGAMTLALLLSATSLSALASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKITSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESMFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALEKVSMKSPWQHKV
                     TLTRSGQAFTVNNPTPYYVIISNASAQKNGNPATGFSPLVIEPKTTVPLNVKMDSVPV
                     LTYVNDFGARMPLFFRCDGNTCQVDKDQSLKG"
     gene            complement(45039..47684)
                     /gene="yfcU"
                     /locus_tag="ACHKXP_001886"
     CDS             complement(45039..47684)
                     /gene="yfcU"
                     /locus_tag="ACHKXP_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO7646907.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDVSKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDGKCLKPGQLEGMEIKADLSQSALVISLPQAYLEYTWPDWD
                     PPSRWDDGISGIIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEEFSGDDTQKKWEWSRYYAWRALPSLKAKLALGEDYLNSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     ISGTLHVRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGGFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDSYYSHDGSLAQVDLSANYHEGQYTSAGLSLLGGATLTTHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTDPPVSVSANQ"
     gene            complement(47766..48293)
                     /gene="yfcV"
                     /locus_tag="ACHKXP_001887"
     CDS             complement(47766..48293)
                     /gene="yfcV"
                     /locus_tag="ACHKXP_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7646908.1"
                     /translation="MVMGAFTSTATIAAGNNGTARFYGTIEDSVCSIVPDDHKLEVDM
                     GDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTDT
                     GAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPD
                     LGNFEANTTFQITYL"
BASE COUNT        11896 a        13022 c        11960 g        11416 t
ORIGIN      
        1 tatacacctc attattttgt cattattaag ttattaacag cacaatcgag ccttcccctc
       61 tggcaaaatc ttattctgca gaccttcaaa acaccgtcct gggggagtac attgttctaa
      121 gctgacttcc acggcaggga gtggcgataa cagcaaaaaa ggtcaagatt catgtccccc
      181 attgaaaaat ccagcaaatt agagaatgtc tgttatgaca tccgtggtcc ggtgctgaaa
      241 gaagcaaaac gcctggaaga agaaggtaac aaggtactga aactgaacat cggcaaccca
      301 gccccgttcg gttttgacgc gccagatgaa atcctcgttg acgtgatacg caacctgcct
      361 accgctcaag ggtattgcga ttccaaaggt ctttactccg cgcgtaaagc catcatgcag
      421 cactaccagg ctcgtggcat gcgtgatgtt accgtggaag atatttacat cggcaatggt
      481 gtatcggagc ttatcgttca ggcaatgcag gcattgctga acagcgggga cgaaatgttg
      541 gttcctgcac cagattaccc actatggacc gcggcggttt cgctttccag cggtaaagcg
      601 gtgcattatc tttgcgatga atcctctgac tggttcccgg acctcgatga tattcgcgct
      661 aaaattacgc ctcgtacgcg tgggatcgtt attatcaacc caaataaccc aaccggcgcg
      721 gtatattcca aagagctttt aatggagatt gtggagattg cacgtcagca taatctcatt
      781 atcttcgccg atgaaattta tgacaaaatt ctctacgacg acgctgagca tcactcaatt
      841 gcaccgctgg cacctgacct gctgaccatt acctttaacg gactgtcgaa aacgtaccgc
      901 gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc cgaaaaaaca cgccaaaggc
      961 tacatcgaag gtctggaaat gctggcttca atgcgcctgt gtgctaacgt tcctgcgcaa
     1021 cacgccattc agaccgcgct aggtggttat cagagcatca gtgaatttat tacccctggc
     1081 ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga tcaacgatat tccgggcgtt
     1141 tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga aaatcgacgc caaacgcttt
     1201 aacattcacg acgatcagaa aatggtgctg gatttcctgt tgcaggaaaa agttctgttg
     1261 gtgcaaggga cggcattcaa ctggccgtgg ccggatcact tccgcattgt cacgctaccg
     1321 cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc gtttcctttc tggttatcat
     1381 cagctgtaat cttaatttca ctgccggaga ttgcatccgg cagcgttatc ccgccacaat
     1441 gacctgatga tgtcatcata cgtaaggtca ctatgaaaca gagccatttt tttgcccatc
     1501 tctcccgcct gaaactcatt aaccgctggc cgctcatgcg caacgtgcgg acggaaaatg
     1561 tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc gctggcagct atcaaaaatc
     1621 gaaaatttgg cggtaatgtc aacgccgaac gtatcgcttt actggcgatg taccacgatg
     1681 cctcagaagt gctcaccggc gatctcccta ctccggtgaa atacttcaat tcgcaaatcg
     1741 ctcaggagta caaggctatt gaaaaaatcg ctcagcaaaa actggtcgat atggttccgg
     1801 aagagctgcg ggatatcttt gcgccgttaa ttgacgagca tgcatatagc gatgaagaaa
     1861 aatcgctggt gaaacaggca gatgcactgt gtgcatatct gaaatgtctg gaagaactcg
     1921 cggccggaaa taatgaattc ttgctggcaa aaacgcgact ggaagcgacg cttgaagcgc
     1981 gtcgcagcca ggagatggac tacttcatgg aaatatttgt tcccagcttc catctttcgc
     2041 tcgatgagat tagccaggat tcaccgctgt aagcagccgg agtctgcgtc gcatcaggca
     2101 ataagcgccg gatgcgacat caggctcttg tcaaaacgga aacagcatcg ggatcatcac
     2161 cacacaaacc gccatcacga taatggtgaa cggtaccccc aacttcacaa agtcactgaa
     2221 gctgtaattt cccggaccta aaaccagtgt gttaacaggt gaagaaaccg gtgtcataaa
     2281 ggcggcggat gctgccatcg ccacgaccat cgcgaatgga taaggcgaca cccccatcgt
     2341 tttggcagca gccagcgcaa tcggagccat caacaccgcc gtcgcggtat tagagataaa
     2401 tagcccaata accgccgaca agacaaacaa acaccccagc atcatatgtg gcccgtaacc
     2461 accgccaatg tccatcagcc ctttcaccgc cagcgcgaca cctcccgttt tctgtaatgc
     2521 cacagcaaac ggcatcatcc caacgatcaa aataatgctc ggccagtgaa tggatttata
     2581 ggcgctttca gcatctatac agcggaattt ccccatcagc aggcaggcga tgatagcggc
     2641 aacaggatta ggaatttcat ctgtcagcat taacgccacc attagcacca gacagaaaat
     2701 ggcatggggt gcctggctgt gcgcgggtga tgcttcactc acctcttccg gtaagttcag
     2761 cgctacgaag tcgcggccct gtttggccag cataccgatc agtttccagt tacccacaac
     2821 caggatgata tcgcccagca gcagaggctc atccgccagc gaaccttcca gcgccacgcc
     2881 attgcgcttt agccccacca cattcagtcc gtagcgggta cgaaaaccaa tttcgcgcac
     2941 cgatttacca atcagttctg actcaggaat taatgaaatc tctgccatgc ccacatcaag
     3001 ggcctggtca gaaaaatact cgccgcgcag taccatcggc tccagcaatt gctcactaca
     3061 aaattgccgg agatcgacat cagccgcaga catatcaata agcaaaacgt cacgcgcgcg
     3121 aaattcagaa accccattaa cgttcacgat aacgcgacga aaacgccgcc agcgttcaac
     3181 accgatgacg ttagcgccat aacgctcacg taatttgaga tcatccagcc gttgaccaat
     3241 cattggcgat ccggggcgaa tagccagacg tcgcgcacgc ccggtcagtc gatattcacg
     3301 gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc tgcggggtct gggtatcccc
     3361 tttcagcatg aaacgcatca ctaacatata caaaataccc agcaccagca cgaccaggcc
     3421 aataggtgtt acgctaaaga aactgaagcc gtgatagcct tcacgcagca attcactgtt
     3481 gactaccagg ttcggcggcg tcgccaccag cgtcatcatg ccgctaatca gcccggcaaa
     3541 acttaacggc atcatcagac gtgacggcga cgtttgcata cgcatggcaa cgcttaacac
     3601 cacgggaata aagatagcga caacgccggt tgaactcata aacgcgccaa gccccgcgac
     3661 ggtcagcatc aacaaaacca acattttgat ttcactattg cccgcaactt tgaccagcca
     3721 tgttcccatt acggtggcaa caccggtacg gaccaaacca tcgccaataa taaacaaggc
     3781 ggcaatcagg acaacgttag gatcagaaaa gccggaaaat acttctggga ctgtcagcat
     3841 tccgcttaat gcaaacgcga caataacaaa caaagcgacc gcatccatac gcactctgcc
     3901 cgtcgcaaac aagacgatgg caacggccag taatgaaaga acccaaatca attcaccgtt
     3961 cacaacttat ccttgttaat tgagggggat gacttgattc tgccataaaa aagccccgac
     4021 gagacggggc taaatcatga tcaggtgttt cactgaataa taacatcgcc atttggctgt
     4081 ttggtcacag taatttgctc cagactgtgg aggaccaaat cgacctcatt caggcgcggg
     4141 gtatctgccg gagcgttaac cgcaatgaca tgacaacccg ccgccaggcc agaaagcacg
     4201 ccagcgggag catcttccac caccacacac tcctgcggcg caagccccag cagctgcgcg
     4261 cctaacagat acgcatcagg ttctggtttt ccgcgcttca ctcgctcagc ggttacaaac
     4321 acctctggtg cgggaagccc agctatttta tggcgcgctc gcgctaccgg catggagcca
     4381 gaagtcacaa tggcccacgg aatacctgct ttattcaaat gactgagtaa ggcgattgcc
     4441 cccggaagcg cggtaatacc ttcggtttcc gtggcctcga tgtgctccag acgcgtaaac
     4501 tcggcggcaa tatcagcctc ggatttgccc gccataaaat ggcgcagaga ggtgatcgcc
     4561 tgtttaccgt gaatgaaagc cagcacctct tccggcgcta acccatgacg tctggcccag
     4621 ttgctccacg cccgttctac cgcaggcagg gaatccacca gcgttccatc aagatcaaac
     4681 agaaaacctt tgcaccgcac gcgggcctcc tcaggcattg ataatttgat taatttcgtt
     4741 ggcgctcaaa tggtactgac gcgggcaggc atgccacaca ttaagcatgc gctgatattt
     4801 ttcccacatt ggcgtctggg cgttaaaacc gtgagttccg gcgtcaaaat gggtatagcg
     4861 cccttccaca ttaaccataa agcggacata accgaggtaa cgtgcttcag tggcggcgtc
     4921 aaagccgagg aaggtgacac gacgttcatc gatggattgc tggtcctgca aattagacca
     4981 ggaaacatgc aacgcatgat acatctccat aatgtcgatg atagtgcggc aggtttcttc
     5041 tttcagctcg ccaaactcgc gatccaattc acgcatctgt aatccgtaac cacgctcaat
     5101 aattgtttgc aggcgacggt aacgttcagc atttgccgga tcgagcatag tcatcatctt
     5161 gtactgatta gacaaaataa gacgttgcgc gttggtcatt tccattgttg actcctgtat
     5221 cactctacta cggtgaaaaa aaagaaggct gagtatgcct tcttttatat gcgtaatcag
     5281 gggtcaatta caaatcatca aggaaagttt tatccagttg tttgaaggcg cgcttaagcg
     5341 tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc tgtccagact
     5401 cctgcaattt accgcgaact tcataaaacc agttaaggat tgcagggggt aatggcgtga
     5461 cagaacgctt gcccagccac cacaatccct gcatgggtaa acttaaggcg aacagggcag
     5521 tggcaactgc cggcccaagc tgaccgccca gggcaatctg ccagcagaga gtaaatacgg
     5581 cgatcggcgg cataaaacgg atcgcataac gcgtcatctt gataacgcga ttttcgacaa
     5641 agaccggggc aaggcgtttt tccagcggcc acgtctttga gtaatgctgt ccccggcgaa
     5701 acaagctaaa aaaattaaca gaacgattat ccggcgttga catgcttcac ctcaacttca
     5761 catataaaga ttcaaaaatt tgtgcaaatt cacaactcag cgggacaacg ttcaaaacat
     5821 tttgtcttcc atacccacta tcaggtatcc tttagcagcc tgaaggccta agtagtacat
     5881 attcattgag tcgtcaaatt catatacatt atgccattgg ctgaaaatta cgcaaaatgg
     5941 catagactca agatatttct tccatcatgc aaaaaaaatt tgcagtgcat gatgttaatc
     6001 ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt tttagccacg
     6061 tatcaattat aggtacttcc atgtcgagta agttagtact ggttctgaac tgcggtagtt
     6121 cttcactgaa atttgccatc atcgatgcag taaatggtga agagtacctt tctggtttag
     6181 ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa aatggacggc aataaacagg
     6241 aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct caactttatc gttaatacta
     6301 ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac
     6361 acggcggcga aaagtatacc agctccgtag tgatcgatga gtctgttatt cagggtatca
     6421 aagatgcagc ttcttttgca ccgctgcaca acccggctca cctgatcggt atcgaagaag
     6481 ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc
     6541 accagactat gccggaagag tcttacctct acgccctgcc ttacaacctg tacaaagagc
     6601 acggcatccg tcgttacggc gcgcacggca ccagccactt ctatgtaacc caggaagcgg
     6661 caaaaatgct gaacaaaccg gtagaagaac tgaacatcat cacctgccac ctgggcaacg
     6721 gtggttccgt ttctgctatc cgcaacggta aatgcgttga cacctctatg ggcctgaccc
     6781 cgctggaagg tctggtcatg ggtacccgtt ctggtgatat cgatccggcg atcatcttcc
     6841 acctgcacga caccctgggc atgagcgttg acgcaatcaa caaactgctg accaaagagt
     6901 ctggcctgct gggtctgacc gaagtgacca gcgactgccg ctatgttgaa gacaactacg
     6961 cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca
     7021 tcggtgccta cactgcgctg atggatggtc gtctggacgc tgttgtattc actggtggta
     7081 tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg caaactgggc gtgctgggct
     7141 ttgaagttga tcatgaacgc aacctggctg cacgtttcgg caaatctggt ttcatcaaca
     7201 aagaaggtac ccgtcctgcg gtggttatcc caaccaacga agaactggtt atcgcgcaag
     7261 acgcgagccg cctgactgcc tgatttcaca ccgccagctc agctggcggt gctgttttgt
     7321 aacccgccaa atcggcggta acgaaagagg ataaaccgtg tcccgtatta ttatgctgat
     7381 ccctaccgga accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc gtgcaatgga
     7441 acgcaaaggc gttcgtctga gcgttttcaa acctatcgct cagccgcgta ccggtggcga
     7501 tgcgcccgat cagactacga ctatcgtgcg tgcgaactct tccaccacga cggccgctga
     7561 accgctgaaa atgagctacg ttgaaggtct gctttccagc aatcagaaag atgtgctgat
     7621 ggaagagatc gtcgcaaact accacgctaa caccaaagac gctgaagtcg ttctggttga
     7681 aggtctggtc ccgacacgta agcaccagtt tgcccagtct ctgaactacg aaatcgctaa
     7741 aacgctgaat gcggaaatcg tcttcgttat gtctcagggc actgacaccc cggaacagct
     7801 gaaagagcgt atcgaactga cccgcaacag cttcggcggt gccaaaaaca ccaacatcac
     7861 cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta ctcgcccgga
     7921 tctgtccgag attttcgacg actcttccaa agctaaagta aacaatgttg atccggcgaa
     7981 gctgcaagaa tccagcccgc tgccggttct cggcgctgtg ccgtggagct ttgacctgat
     8041 cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg accatcatca acgaaggcga
     8101 catcaatact cgccgcgtta aatccgtcac tttctgcgca cgcagcattc cgcacatgct
     8161 ggagcacttc cgtgccggtt ctctgctggt gacttccgca gaccgtcctg acgtgctggt
     8221 ggccgcttgc ctggcagcca tgaacggcgt agaaatcggt gccctgctgc tgactggcgg
     8281 ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta ccggcctgcc
     8341 ggtatttatg gtgaacacca acacctggca gacctctctg agcctgcaga gcttcaacct
     8401 ggaagttccg gttgacgatc acgaacgtat cgagaaagtt caggaatacg ttgctaacta
     8461 catcaacgct gactggatcg aatctctgac tgccacttct gagcgcagcc gtcgtctgtc
     8521 tccgcctgcg ttccgttatc agctgactga acttgcgcgc aaagcgggca aacgtatcgt
     8581 actgccggaa ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg ctgaacgtgg
     8641 tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg cagcgtctca
     8701 gggtgtagaa ctgggtgcag ggattgaaat cgttgatcca gaagtggttc gcgaaagcta
     8761 tgttggtcgt ctggtcgaac tgcgtaagaa caaaggcatg accgaaaccg ttgcccgcga
     8821 acagctggaa gacaacgtgg tgctcggtac gctgatgctg gaacaggatg aagttgatgg
     8881 tctggtttcc ggtgctgttc acactaccgc aaacaccatc cgtccgccgc tgcagctgat
     8941 caaaactgca ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc tgccggaaca
     9001 ggtttacgtt tacggtgact gtgcgatcaa cccggatccg accgctgaac agctggcaga
     9061 aatcgcgatt cagtccgctg attccgctgc ggccttcggt atcgaaccgc gcgttgctat
     9121 gctctcctac tccaccggta cttctggtgc aggtagcgac gtagaaaaag ttcgcgaagc
     9181 aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc tgcagtacga
     9241 cgctgcggta atggctgacg ttgcgaaatc caaagcgccg aactctccgg ttgcaggtcg
     9301 cgctaccgtg ttcatcttcc cggatctgaa caccggtaac accacctaca aagcggtaca
     9361 gcgttctgcc gacctgatct ccatcgggcc gatgctgcag ggtatgcgca agccggttaa
     9421 cgacctgtcc cgtggcgcac tggttgacga tatcgtctac accatcgcgc tgactgcgat
     9481 tcagtctgca cagcagcagt aatctcgtca tcatccgcag ctttgcgctg cggatatctg
     9541 aaccggaaat aatcactatt tccggttttt tattctctta atttgcatta atcctttctg
     9601 attatcttgc ttaactgcgc tgcatcaatg aattgcgcca tcccactttg catacttacc
     9661 actttgtttt gtgcaaggga atatttgcgc tatgtccgca atcactgaat ccaaaccaac
     9721 aagaagatgg gcaatgcccg atacgttggt gattatcttt tttgttgcta ttttaaccag
     9781 ccttgccacc tgggtagttc cggtggggat gtttgacagt caggaagtgc agtatcaggt
     9841 tgatggtcaa acaaaaacac gcaaagtcgt agatccacac tcatttcgca ttctgactaa
     9901 cgaagcaggc gaacctgagt atcaccgcgt acagctgttc acgacgggcg atgaacgccc
     9961 gggcctgatg aacttcccgt ttgaaggatt aacctcagga tcgaaatacg ggacagccgt
    10021 tggcatcatc atgtttatgc tggtgattgg cggcgcgttt ggcattgtga tgcgtacagg
    10081 aaccattgat aacggtatcc tggcgcttat tcgccatacc cgcgggaatg aaattctctt
    10141 tattcctgcg ctgtttattc tgttttcact tggcggcgcg gtatttggta tgggagaaga
    10201 ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc cggctgggct atgacagtat
    10261 taccaccgtc ctggtgacct atattgccac gcaaatcggt tttgccagtt cgtggatgaa
    10321 cccgttttgt gtggtcgttg ctcaggggat tgccggcgtt ccggtgcttt ctggctccgg
    10381 gttgcgcatc gtggtgtggg ttatcgccac tctgattggc ctgatcttta ccatggtgta
    10441 cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg accgcttctt
    10501 tcgtgaaaag caggctgatg ttgaacaacg tccgtttacc tttggtgact ggctggtatt
    10561 gattgtcctg accgccgtaa tggtctgggt gatttggggc gtgatcgtta atgcctggtt
    10621 tattccagaa attgccagcc agttcttcac catgggtctg gtgattggca tcatcggcgt
    10681 tgttttccgc cttaacggca tgacggttaa taccatggct tcatccttta ccgaaggggc
    10741 gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc aaagggattt tgctgctggt
    10801 cggtaatggt gaagcgggtg atgccagcgt gttaaatacc atcctcaaca gcattgccaa
    10861 tgccattagc ggtctggaca acgcggtcgc ggcctggttt atgttgctct tccaggcggt
    10921 atttaatttc ttcgtgacgt ccggttctgg tcaggcggcg ttaaccatgc cgttactggc
    10981 accgcttggc gatctggtcg gtgttaaccg tcaggttacc gtgctggcgt tccagtttgg
    11041 tgatggcttc agtcacatca tttacccaac ctcagcgtcg ttaatggcaa cgctcggtgt
    11101 gtgcagggtg gacttccgta actggctgaa ggtgggcgcg accctacttg gactgctgtt
    11161 tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg ggctaccact aaaaatgtta
    11221 agagccgcaa ttgcggctct tttttcattc tgccgtttca gtctctacgg cttcattttt
    11281 ggcattgcgc ttcatccaca acgctagcgc tttcagcgag tctggagtga actcatcgca
    11341 gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca ctgacttcat cttcctgtag
    11401 tgcgaaggga ccgtgagaga cgcagctgaa caatgcgccc cagacacggc aatttttatc
    11461 ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg gcaatgccca actcttcttc
    11521 cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc tggactacac cgcctgcggt
    11581 cgcatctaac atgccgggta aaaagtcttt tgtctcggta cgacgctgga ccagaatttt
    11641 gcccatgcca tcatgcacga cgatgtaagt tgcacgatga cgcagacact gtgcccgcat
    11701 ttgttcccgg ctggcttgtg caatgacttc gttctcttca ttgacaatat ccacccattc
    11761 agtacttgcc aaacgacgct gttccattat caggaaacct tctttttctg gcgctcttac
    11821 ggcgcatttt gagttgtggg taaattacgg attaatcgcg acctgcgcaa tgatactttg
    11881 atcattgagt gcgattacac taagtacgtc attatccagc atgccataac tcgccggatt
    11941 accgcctttc ggaatactca ccgagccggg gttgaagtgg aaaatctccc cccgctgttc
    12001 tgccactggc agatgggtat gaccgtacac cagcacatcg ttctggttta aagcaggcag
    12061 attttccggg ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt ccagtaatac
    12121 ctgttgccac ggcgcggtta tcgggaaatg cagcagcatt tgatccactt cgctgtcgca
    12181 gttgccgcgc acagcgataa ccttatgtgc cacttcatta agccgttcag cgactttggc
    12241 tggcgcgtaa ccctccggta aagcattacg cgggccgtga ttcaacacgt cgccaaggat
    12301 caccagccac tgggcaccgc tttgggcaaa caactccaga acacgttccg tcgccggtaa
    12361 cgacccatga atgtccgatg caaacatcag tttcatcact cactcctcgt cgaaaaaacg
    12421 taagctatga tactggattc tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa
    12481 taaaacgctg gacagacgct cgctgccact ggaatgttgc ataatccacc aggcgttctg
    12541 gcacctcatc gccatgtagt accaggcggt taatcattag cgccagatca gtatcagcaa
    12601 tgcaccattc accaaataaa ttcggctgac ccagtactaa caaatgttct gccatcgcga
    12661 acagtttctc tgcactggct tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa
    12721 agacaacatc cgtcggacgc tcttcgcgga tgggcatcag atcgctgcgc agccaggcct
    12781 gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg ataaatacgt tcccaggtcg
    12841 gtggcgcaaa tcgatcttcc agatactccg caatggcaga agattcactc aactcaaaat
    12901 catcgatttg taataacggc acacggcgtg tctgaccgta accttgccac gtcggctgca
    12961 aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa cgacaggcct ttttcctgca
    13021 acgccaccca ggcggataac acataagggg agaaaaagtg ggcatctgac caaagcgtga
    13081 tagcgggttt actcattatg ttctcggcaa taaagggggc tggctatcaa actatagagc
    13141 ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg ccgcgctttc tatacttgtg
    13201 gtgttcagat gattaaacaa ccggagctgc aatgatcgat ctctatttcg ccccgacacc
    13261 caatggtcac aaaattacgc tgtttctcga agaagcagag ctggattatc gcttgattaa
    13321 ggtagacctg gggaaaggcg gtcagtttcg tccggaattt ttgcgcattt cgcccaacaa
    13381 caaaattccg gcaattgttg atcattctcc tgccgatggc ggcgaaccgc taagcctctt
    13441 tgagtctggt gccattttgt tgtatctggc tgagaaaaca ggactctttt tgagtcatga
    13501 aacgcgtgag cgcgccgcca cattacagtg gttattctgg caggtaggcg gactggggcc
    13561 gatgcttggg caaaatcatc attttaatca cgcagccccc caaaccattc cttacgctat
    13621 tgaacgttat caggttgaaa ctcagcgtct ttaccatgta ctgaacaagc ggctggaaaa
    13681 ctcgccctgg ctgggaggcg agaactacag cattgcggat attgcctgct ggccgtgggt
    13741 taatgcctgg actcgccagc gaattgacct agcaatgtat ccggcagtca agaactggca
    13801 tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg ctaaaagcac aactcggtga
    13861 tgagcgttcg gatagttaac agaaacaggt tctcgtgtat tatttcatcc taagtaaaac
    13921 aacggagaac ctgcaatggc acaacctgcc gctattattc gtataaagaa ccttcgtttg
    13981 cgtacgttta tcggaattaa ggaagaagaa attaacaacc gtcaggatat tgttatcaat
    14041 gtgacgatcc actaccccgc cgataaagcg cgcactagcg aagatatcaa cgatgcgctg
    14101 aattatcgca ccgtaacgaa aaacattatt cagcatgtag agaataaccg tttctctttg
    14161 ctggaaaaat taactcagga tgtgctcgat atcgcacgtg aacatcactg ggtgacgtat
    14221 gctgaagtgg agatcgataa actgcacgcg ctgcgctacg ccgattcggt atccatgacc
    14281 ttaagctggc agcgttaatc gccatatcgg gaggctgcat gaacatagtg atcaccggag
    14341 ggacaggatt aattggtcgc catttgattc cgcgtttgct ggagctgggc catcaaatta
    14401 cggtagtgac gcgtaacccg cagaaagcca gttccgttct cggccctcgg gtgacactat
    14461 ggcaagggct tgccgatcaa agcaacctca acggcgttga tgcggtaatc aacctggccg
    14521 gagaaccgat tgctgataaa cgctggactc acgagcaaaa agagcgtctc tgccaaagcc
    14581 gctggaatat cacgcaaaaa ctggtcgatt tgattaatgc cagcgacacg ccaccgtcgg
    14641 tactcatttc cggctcggca acgggctatt atggtgactt aggtgaagtg gtggttaccg
    14701 aagaggaacc gccgcataac gaatttaccc ataaactctg cgctcgctgg gaagaaattg
    14761 cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct gcgtaccggt gtagtgctgg
    14821 caccggatgg cggtattctc ggtaaaatgc tgccgccgtt tcgtcttggc ctgggcgggc
    14881 cgattggctc tggtcggcag tatctggcct ggattcatat tgatgatatg gtcaacggta
    14941 ttctctggct gctggataac gagctgcgcg ggccatttaa tatggtttcg ccctacccgg
    15001 tacgcaatga acaatttgcc catgcgctcg gtcatgcgct gcatcgcccg gccattttgc
    15061 gcgtccccgc gaccgccatt cgactgttaa tgggcgaatc ttcagtactg gtattaggtg
    15121 gacaacgcgc gctgcctaaa cggctggaag aagcgggttt tgcgtttcgc tggtacgatt
    15181 tagaagaggc gctggcggat gtcgttcgct gatgtggttt acagcaaaca tccgccagtt
    15241 aactcccggt gttacaggat tagtggcttt gcgcgataag atcgtctggt gaaagtcggg
    15301 tcaccatcat aactaactct ctgtctaaac ctctatccag catctcctga gcaatacgca
    15361 gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg taatctgtca gcaatggtca
    15421 tcagtttctc cttttcttgt ggtgcgcgtt ccgctatctc accaataaat gcacgaaaac
    15481 gctgggcatc ccctgtttgt aatacgtaat taaacagggc ttttagctgt ctgtcattag
    15541 tgttccctgt aactagcagc gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac
    15601 gaatatgttt ctgaattaac tccaacagcg ccattttgcg gtgttgcata atctcgtcat
    15661 ccggcaccac ggtaatatcc accaacggaa aagccgatga atatattttg cgggctatag
    15721 caggctcggc aaattcatca agccagcaga gtgaataagg ataaggactt ctgcaaccat
    15781 gataaaacag catcgggatc accaatggaa gctctttata gcccgcatca agatggtttt
    15841 gcattgccgc aatggaataa cgcatcatgc gaaaagccat taattcttcc ggcttacttt
    15901 ggtgctctat cactacataa atataaccca ctccctcctg cgttttcaca gaccacaaga
    15961 ggtcggaata atattgccgc aggtcttcat caataaaact gtttggttcc agtttaagcg
    16021 tcgttaaatc acacagtttg cgcagcggcg cgggaagatg aatatcaata aaatcccgcg
    16081 cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc atgcggcgtg gaagttgtcg
    16141 atattgtcat ggcgttccgt cacccctcaa atttaagatg acgcgacaat aaccacgatt
    16201 aacacccaat gcagcaggca gtttttatct ttggacagcg aattcagaag tatttttagc
    16261 ggactgtaac ttttacattt cagcggaagc tgactcgcaa attccccatg aaaatactcc
    16321 ggcagcgtta gccaccggag aggaaattat ttcagcgatc ccttaaggaa ccgttgcaga
    16381 cgagggcttt gcgggttgcc aaataactgc tccggcgcgc cctcttcttc tattttcccc
    16441 tgatggagga aaatgacatg agtagaaaca tggcgagcaa agcccatttc gtgagtcact
    16501 accaccatgg ttttcccctc ttctgccagt tgctgcataa tacgcaacac ttcgcctacc
    16561 agttcaggat cgagcgccga ggtaggctca tcaaacagta aaacttccgg ttccatcgcc
    16621 agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg aaagatgcac cggatatttc
    16681 ccctgcgcac gttcgtctat cccgactttt gccagatact tcaccgcccg ctcgcgcgct
    16741 tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca tgacgttttc cagcaccgtc
    16801 atatggctcc agagattgaa gtgctggaat accatcgtca ggcgtgtgcg cagtaagcgc
    16861 agttgatttt tatcggcgac tttgagttga ccgtctttgt cgcgcaccag attgatcgtc
    16921 tggccattga ccacgatcga cccttcactc ggtttttcga ggaagttaat gcagcgcaga
    16981 aaggtacttt tccccgatcc cgacgatccg atgatgctta ttacatctcc ggcattcgct
    17041 tgcagtgata cccctttcag cacttcatgt tcgccgtagc gtttgtgcaa atcgataacg
    17101 tttaatttat tctcggacat cgtattctca gtgcgttgaa gaaggtttca catgctgcaa
    17161 ccagcgtttt tccgctctgc gaaagaggct gatcaggaca taagagatga ttaaatagag
    17221 caccgcggca atgccgaagg cggtaaaagg ttgatacgtg gcggcgttaa tatcgcgggc
    17281 tattttcagc agatccggca ccgtggcagt aaatgccaac gcagtagagt gcagcatcag
    17341 gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc gcagaaggca aaataatgca
    17401 gcgatacatt ttaaaagtcg agaagccata ggctctggcg gcttcaattt ccccatgcgg
    17461 aaccgaacgg attgccccag caaaaatctc ggtagtgtaa gcgcaggtgt taagcgtcag
    17521 cgccagcacg gtacagttca ggccactgcg gaagaaagcg ttaaggaatt cggttccctt
    17581 aacaatctca agcgtgtaca tgccggaata gaacaccagc aactgaacat acagcggcgt
    17641 accgcgaaaa atataggtaa ataaccagat tggaaactgg atgtatttat tactggagac
    17701 acgaccaatc gccagaaaca gcgccaggac tccgcctatc actaccgaca aaataagcag
    17761 ccacagagtg atcgccacac cagtaaagcg ataaccgtcg gtccacagca gcggtttcca
    17821 gtattcatgt aagatttcga tcacaggtca gccctcttca cacccacgga gtagcggcgc
    17881 tcaaggaaca gcagcacacc attggaaaca gtggtgaaaa ccaggtaaat cacgccacag
    17941 acgatggcga aatagaacgg ttcccaggta cttttgcctg ccagttgggt ggctttgacc
    18001 acatcttcca ggccgagtaa cgaaaccagt gcggtagatt tgaggatcac ctgccagttg
    18061 ttgccaatgc ctggcagggc gtaacgcatc atcgacggaa acatgatccg ccgaaacact
    18121 tgcccacgag taaaaccgaa cgccgtcgcc gcctctatat gtcctttcgg cactgccata
    18181 aaagcaccac ggaacgtttc ggtaaaataa gcaccgtaga taaaaccgag agtgataata
    18241 ccagcgacca tcggatcgat atcaatctgc ccgacgccca tcgcctccgt caccgtgttt
    18301 agcgcaatct gcaaaccgta gaaaatcagc agcatcaaca ctaagtccgg cacgccacga
    18361 atcagcgtgg tgtacccttc gaaaatcagc cccgaaagcc gattttgcga gagcttaccg
    18421 ccagcgccaa ttaaaccgat gattacagcg agcactacag agctgatagc cagctccagc
    18481 gtgacgagcg caccctgtaa aataacacct gaaaacccat acaacatgct gcctgtcctg
    18541 tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt acccgaaggg agcgggcttt
    18601 ctttcaccga tggcgattag ccaccataaa catcaaaatc gaagtacttt ttcgctaatt
    18661 tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt gttcagtgct tcgcgcagtt
    18721 cgttatcttc tttacgcagg cccatgccgg tccctacgcc aaacagtttt tcatctttaa
    18781 cagacgggcc accgaatttg taatctttac cgacaggttg tttgaggaaa ccttcgctgg
    18841 cagcgacctc atcctggaac gcggcatcaa tacgtccggc agtcaggtca gaataaatgt
    18901 tgtcctgccc ctgatacgag acgatttcaa tgccttttgg tgcccaatgt tcattaccga
    18961 acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt gcctttcagc gactcgactg
    19021 tcggctgaat gtcagaattt ttcgccacca ccaaacgaga atcggcagcg tacagtttgt
    19081 cggtgaaggc tatttcttgc tgacgttttt ccgtaatgga aagcgatgac atgatggcgt
    19141 caatcttctt cgcttttaag gacggaatta acgcatccag cggattttcg acaaacgtac
    19201 attgcgtatt gatgcgtttg cataattcct ttgccagatc gatatcgaag ccaaccagtt
    19261 cgccttgtga attttttgat tcaaatggcg cataggtcgg gtcggtaccg atgcggatgt
    19321 tttgcggaat cgcagcaaac gccgcagttg cgctggagaa ggccagaacc agagagagcg
    19381 atagcaccag ttttttcata atgtcctcaa ctgacagtct ttttataggg aatatttaca
    19441 ggtttgttac acttatcgtg ccataaaatt gaccatcaag gcaaaatatc ccgccccgtt
    19501 agacgtatta tttttcatta aaaatgctta agtatgcatt tcaatgcacc acgatagtgc
    19561 aacaaacctg gcgtgcacca tgatgatcat tccatcaggt acagcttccc agcgacgtat
    19621 cagtcaccgt agacattaaa gtcgaaatac tttttcgcca tcttgtcgta ggtgccgtcc
    19681 tgacgcagct cgccaagcgc cttattgaag gcagccgtca gttcagcatc atctttacgt
    19741 agccctacac cggtgccatc accgaagtat tttttgtctt ttactgatga gccagcaaag
    19801 gcgaaatctt taccagcagg ttgcttgagg aatccttcgc tggcagcaac ttcatcttgt
    19861 aacgcagcat ccagacgtcc tgcagccaga tcggaataga ccaaatcctg gttggcatag
    19921 gccaccacat ccacgccttt actacgccag gtctcgttag cgtaagcttc ctgggttgat
    19981 ccctgcagca caccaacatg tttacctttc agtgaatcca gcgttggctg aatcggtgaa
    20041 cctttggccg caatcaaacg agaatctgcg gcgtacagct tgtcggagaa ggcaatctcc
    20101 tgctgacgtt tatcggtaat ggaaagcgac gaaataatag cgtcgatttt tttcgctttc
    20161 agtgagggga tcagcgcgtc aaagtcactg gcaacccagg tacatttcac ctgcatccgt
    20221 ttgcacatct cgttaccgag atcgatatca aagccaacaa aatcaccttt agcatctttc
    20281 gatgagaacg gtgcgtaggt ggtatcggtt ccgatacgta ccgtctccgg tagcgccgca
    20341 tagctggaag ccgctgtgga gagaccgact aacaaagaca gagcgagaat cgacttcttc
    20401 atacataacc ctcaagtgaa aatggctttt ttatgttgtg tattgtgttg tgtgtttgca
    20461 ggctctttca tgcaggtctt atgccatctt gacgacaaca gtaacattca acgttaaata
    20521 tgttaataag acgttgcatt attgtcctga agttgaagat agcaggtata gcggttgaat
    20581 cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg atttgatcgc ggttgcaaaa
    20641 ttgccctgaa acagggcaac agcggagtta tgcgccctgc cagcgggcaa agagatcttc
    20701 aggaagggtt atcgcaaact ggtcaagaac acgattaacc gtctgattta tcacatcatc
    20761 aagggattgc gggcgatgat aaaacgccgg aacgggaggc ataatcaccg caccgatttc
    20821 tgccgcctga gtcattaaac gcagatggcc taagtgcaat ggtgtttcac gcacgcagag
    20881 caccaacggg cgacgctctt tcagcaccac atctgccgca cgggtcagta agccatcagt
    20941 atagctatgg acaatgccgg aaagggtttt gattgaacag ggtaaaatca ccatccccag
    21001 cgtctggaaa gaaccggaag agatgctggc ggcaatatcg cgcgcatcgt gcgtgacatc
    21061 ggctaatgcc tgcacttcgc gcagagaaaa atccgtttcg agggataagg tctggcgcgc
    21121 tgcctggctc atcaccagat gcgtttcgat atctgtgaca tcgcgcagaa cctgtaataa
    21181 gcgcacgcca taaatcgcgc cgctggcacc gctgatgcct acaatgagtc gtttcataaa
    21241 tagttgccct gtcagacttg caggcagact ttgcaggatt tcggcgggag ttgcaagtca
    21301 gggtgccaga ccggcaccct cagcgaaggc atcatccttc gttatgcatt tcgagatttt
    21361 ccacttcgtt ctgacgttgc actgctttgg cgtcatcatt acgtaacgta tcgaggaaat
    21421 cgaggtagcc ctgatcaaca tctttggtga cgtagacgcc gttgaacacc gagcattcaa
    21481 actgctggat atccggattt tcagcgcgaa cggcgtcgat cagatcgttc agatcctgga
    21541 aaatcaaccc gtcagcaccg atgatctggc gaatttcatc aacttcgcga ccgtgagcga
    21601 tcagttccgt ggcgctcggc atatcaatac cataaacgtt cgggaagcga atttccggtg
    21661 ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg tgccatctcg ataatctgct
    21721 cagaagtggt gccacggacg atggagtcgt cgaccagcag gacgttttta tcgcggaact
    21781 cggcgcggtt ggcattcagt ttacggcgca cggacttacg acgcagctgc tggcccggca
    21841 tgataaaggt gcggccaaca tagcggtttt taacgaagcc ctggcggtac ggtttgccca
    21901 gaatacgagc aatttccagc gcgatatcac acgaggtttc tgggatcggg atcaccacgt
    21961 cgatatccag atcttcccat tcgcgggcaa ttttctcgcc cagtttcgtg cccatattca
    22021 cacgcgcgct gtaaacggaa attttgtcga taaacgagtc cgggcgggca aagtatacat
    22081 actcaaacag gcacggattg ctgaccggat tgtcagcaca ttgacgggta aacaactgcc
    22141 cttcttcagt gatgtaaatc gcttcgcccg gcgcgacgtc acgcaggaaa tcaaagccca
    22201 gcgtatcgag cgctacgctt tcggaagcga ccatatattc tgtacggttc tcgtcaatat
    22261 cacgttttcc cagtaccagc ggacgaatcc cgtttggatc gcggaaagca accataccgt
    22321 ggccgataat catcgccaca caggcatacg cgccgcggat taagcggttt gtggcagcaa
    22381 tggcagcgaa aatattgtcg gcttccagcg ggtagtggcg gaagttgtcc agctcgctgg
    22441 cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt gatgtggcgg cgtttttctt
    22501 caaacagttt tttacgcaac tcgtgagcgt tggtcagatt gccgttgtgg gcaagcgtaa
    22561 tgccatacgg ggagttaacg taaaacggct gcgcttcaga ggcgctggag ctgccagccg
    22621 tggggtaacg cacatgacca atgcccatat tgccctgcaa acgctgcata tggcgagctt
    22681 caaatacatc gctcaccagc ccgttcgctt tacgcaaacg gaagcagtta ttggcatcta
    22741 tggtgatgat gccggcggca tcctgaccgc gatgctgaag caccgttaag gcatcataaa
    22801 tcgactggtt aaccggcata acaccggcga taccgacaat accgcacata cgtctttttc
    22861 ctcgttaagc cacatctcag agcacttacg ctctgggcaa gaaacttgac gagctttgca
    22921 gataatcaaa aaaccatctg atgataaaac tgaattgcgg gatcagctgt gatttgctcc
    22981 agtcttcgct tttcgacacc ccggtaaagg agtcgagaaa gaagagaatg gcagcaacaa
    23041 tcaacacacc gcgcaacgca ccgaaacaga cgcccagcac ccgatcggtg cctgacaacc
    23101 ccgttttctc caccaactgg cctatcacga agttcacgat agcaccaacg atcagggtag
    23161 cgataaacag taccgcgatg gcaatcccat ttcgaaccag ttcgtcttca aagcccgtaa
    23221 accagactga caggtaagtg tagtaatgac tggcaacaaa gaaagcacaa ccccatgtca
    23281 ccagcgataa cgcttcacga acaaagccgc ggatcaggct aaccagagag gaaaaagcaa
    23341 tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt cccacgattt tacgccctgt
    23401 catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt ttgcgtaggg atttccttcc
    23461 cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc catcgacttt ttatgccttt
    23521 gcggcatcgg gcaatgcgtg tcggatgcgg cgtaaacgcc ttatccgacc tacggttcta
    23581 cccctgcgta ggcctgataa gacgcgccag cgtcgcatca ggcaagaccg tattaattcg
    23641 gcgtatagcc cattaccacg ccacttaagc cagaaagttg cttcaactca cccagcgaac
    23701 ctttcagctt atctttcgag gcatccggcc caaccagaat acgggtaatt ttaccctgca
    23761 ctggcgtgga tggcgacgta taaacccgat aaccggcacc gcgcagctta ccgacaatct
    23821 cattcacttt atcggcattt ttcagcgcac ccagttgcac aacataggct ttacccgtcg
    23881 gtgcagcttt ttcttccacg accggcttcg gttctggctt cggcgcaggt ggtgcttcaa
    23941 cctttggttt aggcggctcc accggtttcg gcttcggtgg ggcgaccggt gcaggttccg
    24001 gttcaaactc ggtgttattg gctgcaatag tggccggatc gagcgacggt gctgcggcat
    24061 cacctgcccg cacctcttcc gctgcgcctt ccggcggctg cgtcggtaac gcctgggtgg
    24121 cggctggcat catatcaggc tcatcacgat cgcccgcttt cggcaccagc gggatagccg
    24181 cgaactcatc ctgataatgt tttttctgcc cgtccagcag ccctggaagt acaatcaccc
    24241 ccagcgccac cagcacgatc gtgcccacta accgattctg aaacttactt gccaccgctt
    24301 ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt ggaaagaacc acacaccagc
    24361 acggtgtctt ccgctttagc gtccgccatt gcggcatccc atgcctgcgc aacgctatca
    24421 aatgatttgc cgttacccaa atgctcaagc agttgttctg ccgtggcacc gcgcggccct
    24481 tccagtggcg cacaatacca gtcatcaacc acgcttttca accaggccag agttccggca
    24541 atatctttat catgtagcat accgataacc gccagcacgc gcccgttttt cggtagcgct
    24601 ttcatacgcc cggtgagata ttccgccgca tgtggattat gcgcgacatc aaaaataacg
    24661 cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa ttgcgctggc aatcccgtcg
    24721 cgaatggcat tttcactgac ttccagcccg ctggcacgca gtgccgccag cgctgttgcg
    24781 gcattcggtt gcgggacaag cggcaacggc agattttcca gcgtgccgtg agcatcgcta
    24841 aacgcccagt catgatcggt gacggaatag ttccactcaa cgccccgacg ttgtaacagt
    24901 gcaccttttt cctgcgccac atcagcaatg gtagaaggca tttccggctc accgacaatt
    24961 gccggttttt cgctgcggaa gatgcctgct ttctcgcgac caatactttc gcgatctgga
    25021 cccagccagt cggtatgatc cagcgcaata ctggttacta ccgcgacatc ggcgtcgaca
    25081 atattggttg cgtccagacg accgcccagc cctacttcca gaatcaccac gtcaagttgt
    25141 gcctgcttga acagccacaa cgccgacagc gtaccgtact cgaaataggt cagggaaata
    25201 tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt gggccgattc cggcaattcc
    25261 tggccctgca cacgtacgcg ctcggtataa cgcaccagat gaggcgaact gtagacgccc
    25321 actttgtacc ctgccgccat cagaatcgac tccagcgtac ggcaggtggt gcctttgcca
    25381 ttcgtacccg caacggtaaa cacaaatggc gctggtttca ggacgccaag acgcgccgcg
    25441 accaggctca cgcgctcaag gccgagatcg atagttttac tgtgcaggtt ttccagataa
    25501 gaaagccacg aagccagagg cgacgcggct tgaggagtgc gtttgataat catggtatcc
    25561 gctgattcgt tacggtgaga atagcaaaag ggcagagcca gtggccctgc ccttatcagt
    25621 tatcaggcct caggttcctg atccggtacc gggggtacca ctacgccttc acgcggcgct
    25681 tcaggattcg gcgctggcag attcatcaac ttcgccagaa tgctcgccag tttcaggcgc
    25741 atttccggac gacggacgat catgtcgatc gcgcctttct cgatcaggaa ttcactgcgc
    25801 tggaatccag gcggcagttt ttcgcgaacg gtctgttcga taacacgcgg accggcaaag
    25861 ccgattaacg ctttcggttc agcgatgttg agatcgccca gcatggcgaa acttgcagaa
    25921 acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg gcaagccgcg ctcctgcatt
    25981 tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg acatcagtgc ttcctgcata
    26041 cgtgcgccac cagaggcgga gaagcagatc agcgggcagt tatcttccag cgcctgctca
    26101 acggcacgca cgaaacgtgc acccacaaca gaccccattg aaccgcccat aaaggcgaac
    26161 tcgaatgccg cagcgacaac cggcattcca tacagagtgc ctttcatcac caccagcgca
    26221 tctttttcgc cggtttcttt ctgcgcagat gccagacggt ctttatactt cttggagtca
    26281 cgaaacttca gcacatcttt cggctcaagc tcgctaccca gctccacaag gcttccttca
    26341 tctaacaggc tatgcaggcg attacgcgct gtcatacgca tgtgatggtc acacttcgga
    26401 cagacctcaa gattacgttc cagctcagcg cggtataaaa cctgaccgca gctatcacac
    26461 ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg gagtaatgtt gcttttaatt
    26521 cgttcaatcc agctcattag ggacctttct gtctgaacct ggttcgatgc cagttttatc
    26581 tttggggacg cataatgcca tttttgcccc caacagacca tgaatgttgc acattaaaac
    26641 ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg cggagttact ttttattttg
    26701 cggcgcgtgc cgcagcgcgt ttgtgacgga ttatttcgat gacgcccggc aaaatagaaa
    26761 ccacaataat cccgacgatc agcagcttaa ggttatcctg aaccatcggg attgtaccga
    26821 agaaatagcc tgcgtaggta aaaagcagta cccacaacag tgcgccgatc acgttataag
    26881 cggcgaaatg acggtacgac atgtggccca ttcccgcaac aaacggggcg aacgttctga
    26941 cgatcggcac aaaacgggcg agaataatcg ttttgccgcc atgtttctca taaaactgat
    27001 gggttttgtc gagataactg cgacggaaaa ttttcgaatt gggattactg aacaactttt
    27061 caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc cacaatcgcg gcgatcagca
    27121 tcagcaccac catcatatgg acgttgagat cgttggtttc cagtgatgcc aacgctccgg
    27181 caacaaacag caatgaatca cccggtaaaa acggcgttac caccagaccg gtttcacaga
    27241 acaaaattaa gaacaaaatg gcataaaccc agacgccgta ctccgcgacc agctctgcca
    27301 gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata aatcaggtcc atatttgcct
    27361 atgccttgta ctcgtcatct ttcaggctgt aactacgttg gctacagcct gaaatgttcc
    27421 gagcattatt ctcgttagtc cgccagaaat agcgggccca ttggcggttt tggaagatca
    27481 taccggtcag ggtaatccac cgcgaccaga tacagccctt ccgcttttgc cgttgctgcc
    27541 gccagcgttc tgtcctttgc cgccagcaac tctgctatcc agctctccgg ctggttgtgg
    27601 gcaccgactt ccatcaggct gccgacaata ttcctgacca tatgatgtac aaaagcattc
    27661 gctttaatat ctaccaccac ataaggaccg tgacgcgtga cattaatatg cataacgttg
    27721 cgccacgggg ttcgggactg gcactgcacc gcacggaacg aggtgaaatc attctcgccc
    27781 agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca gcggttcgta aaaatgggtt
    27841 acccctttac tcagtaccgc cgggcgcagc cgatgattgt agatgatgta gcgataacgg
    27901 cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta cagctttaac ccaacgcaca
    27961 gcgatgtcac caggtaaatt cgcatttacg cccaaggtcc acgccgcgtc tttgcgcagc
    28021 gcggttgttt cgaaatgcac aacctgcccg gtaccgtgta cccctgcgtc agtacgcccg
    28081 gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga gcgccttttc cagcttctcc
    28141 tgcacactgc ggacttcgtt ctgccgttgc cagccgtaat acttactgcc gtcgtactca
    28201 atgcccagcg caattttata aactggcggt tgttgctggt cggacattag tacagatact
    28261 cctgcaccag tttctcggcg atttttactg ccatcagcgc gccgccaaag cgaacgttat
    28321 cggccaccga ccagaactgg acttgctccg gcataccgta gtcattacgc acgcagccaa
    28381 cagaaagatg cggcgtaccc gaagcatcac ctacctgagt tgggaattcg ttctcttcag
    28441 agagcacaat atcttcgcct tgaacaaacg catcacgcgc ttcttctgct gccagtggac
    28501 gcagagcttc aaagttgacc atctgggcat gaccgtagaa taccggtgcc tggacgacgc
    28561 tagccgaaat catcagccct tcgtcctgca ggattttgcg tacttcgtca acgatacgac
    28621 gttcttcacg cacgctacct tcgctatccg gcagtaacgg cagcatgttg aacgccagct
    28681 gacgcccgaa gaaatcttct tcgtcaatcg gaatgccgtt gagcaatttc gcactctgcc
    28741 ccgctaacgc atcgaccgct tttttgccct gggcggaggc tgaaatcagg ctggtaacgc
    28801 tgatacgtga taaaccgccc tgatcgatta acggtttcag tgccgccagc agctggctgg
    28861 tcagactgtc tggtacggcg atgacattcc ggttccggta atctgtcagt acaaacgggt
    28921 ttacttccgg caccaccagc ggtacgtcgg gttcgagagc aaacaatcca ctgctgtcga
    28981 tcaccaggca acctgagttg gtcgcttctt caacccaggc agcggtagct tctttgcctg
    29041 cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc agcggcatcc tgcacggtga
    29101 ttgtcttacc accaaagcgc agttgttcgc ctgcgctttc gttacgtgcc agtgcataaa
    29161 tttccccaac cgggaactga cgttcagcca gcgtttcaag cagggcttcg cccacagcgc
    29221 cagttgcgcc caggacggca atgttccagc cttcagacat ggtggtttac tccagaaata
    29281 gcaaagctcc ctgccaatgt tacagcagag agcatgaaga agagattaac gtgccggatg
    29341 atgaacggcg ttaaaaccca gtttacacag caatgatgcc gcactggcgt catcacaaat
    29401 tacatacaga gatgaccatt cacggcgctc aagatagttt ttgcgcagtt tatcgaactc
    29461 acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg cggcgcacat catacaccaa
    29521 atgcaccagc cttttcagcg tcggttgatc gagcgggcca tgcagcgtaa tgcgaccaaa
    29581 ctctggcgca ggcagtaatg tatccagcgc aacgtgctgt tcatgcccaa taaacttgct
    29641 ataagcttca aacacttgcg tagtaccgcg tgctttacct tccagggtat agcctgcgat
    29701 atgcggcgtg ccgatatcca cttttttcag cagctcgacg ttgagttccg gttcgccttc
    29761 ccagacatcc agcactacgc ttaacttctg gccttcattc aggcaggtca gcaacgcagt
    29821 attatcgacg actgcgccac ggcaggcgtt aatcagaatc gctccgggct tcaggctacg
    29881 gatcagtttt tcatccgcca gatgtagcgt tttgtacgga ccatctttaa agagtggcgt
    29941 atggaaagtc agaatatccg cgcgctggac taactcatcc agcgagcgga aatcaccctc
    30001 atccccacgg tcggcgcgag gcggatcgca aagtaaggtt ttaatcccta acgcttccag
    30061 tcgcgcctgc aatcgacgtc caacgttacc aacgcccacg atccccaccg tacggtcgta
    30121 cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag gagaaaacat attccaccac
    30181 cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt cccgcctgct tcaaccatgc
    30241 ttcatcgaca tggtcggtcc ccgcagtggc agtgccaaca aatttaatgg gttttcctgc
    30301 cagcaaagat tcattcactt tcgtgaccga acgcaccatc agcgcatccg cgtctgccag
    30361 ttgagcgacg gggattggac gcccgggaac cgcggtcacc tcacccaaac ggctaaataa
    30421 gtcgcgggca taaggcatat tttcatcaac aaggattttc acgtttgtgt tacctgtatg
    30481 agacgagagt taaccggaca agtgtgccat aatctcgcgg ccaggcatac ttgcgaagat
    30541 ttcaggtata aggatacgta atgatacaac ctatttccgg ccctcctcct gggcaaccac
    30601 caggtcaggg agataatctg ccgtctggca cgggcaatca gcctttatcc agtcagcaac
    30661 gtacttcgct ggaaagctta atgacgaaag tgacctcact gacgcaacag caaagagcag
    30721 aactgtgggc gggtatcagg cacgatattg gtctgtcggg agattcaccg ctgctttcgc
    30781 gtcacttccc tgccgctgag cataatctgg cgcaacgtct gctggccgcg caaaaaagcc
    30841 attctgcccg ccagctttta gcgcaattag gggagtattt acgtctgggg aataatcgtc
    30901 aggcggtcac ggattatatc cgtcataact ttggtcagac gccgctgaat cagctctcac
    30961 cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg gaagatggtt attccgcaac
    31021 cacagcagcg cgaggcgacc gaccgtcctt tattaccggc ggagcacaat gcgctaaaac
    31081 agctggtgac caaacttgcg gcggcaacgg gggaacccag caaacagatc tggcaatcga
    31141 tgctggaact ttccggggtg aaagatggcg agttaattcc agcgaaactg tttaaccatc
    31201 tggtgacctg gctacaggcg cgtcagacgc taagccagca aaatacgccg acgctggaat
    31261 cactacagat gacgctaaaa caacctttag atgccagtga actggcggcg ttatcggcat
    31321 atatccagca aaaatatggt ctttctgcgc aatcatcgct ttcttctgcc caggccgagg
    31381 atattcttaa tcagctttat caacggcggg ttaaagggat tgatccgcgt gttatgcaac
    31441 cgctgcttaa tccttttcca ccgatgatgg acacgttgca aaatatggca acgcgtcccg
    31501 cgctgtggat actgttagtc gcgattatcc tgatgctggt ctggctggtt cgttaacccc
    31561 gacgaaatga cagtatcgtg acaataatac ccagcaccgc agagatcgcc ccggcaagaa
    31621 ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc cagcggcccg gagacgccga
    31681 gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt gccgcgaact tgtgagggga
    31741 cgcgtttaac cacctccacg cccagcgcag gaaagataag cgaacatccg gctccggtta
    31801 acgccgcgcc cgctaatgcg acccatgcac ctggggcttg ccagagcagc aacaagccca
    31861 ccgtttctac aagcagagag acaatcgcca ctttcacgcc gccaaaacgg tccggcatcc
    31921 agccaaacat gacgcgcatc acgacaaatg cgccgccaaa cgcggtaaga gtaaagcccg
    31981 ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa agtcccgata acagcaaaac
    32041 caacgccttg tagtgccaga cctaaccctg gtttccagat aagcccgaca acgctccaca
    32101 gcgatggacg ttctcccgcc agggccggta ctttgcgcac tgtgccgtta caggcccacg
    32161 ccagtacggg taataccatt gtggtgatcg ccagtgcggc aaaaccgtaa tggctatgaa
    32221 tcaacaggcc aagcggagca ccaacagcga gggcaccgta aatcgccatt ccgttccatg
    32281 acatcacttt gccagagtgt tttggcccta cgatgcctag tccccaggtc agagcgcctg
    32341 tcagtaactg gctttcacca aagccaagaa tcaaacgccc gacgaccaac agagcaaatt
    32401 tgaacggtgc ggagacaggc aaaatcgccg ccagcagcaa cgcgccgcca gccagaccac
    32461 aagctaacat tccctgaagc gccgaacgtt ttgcaccata ttgatcggcc agtcgcccgg
    32521 cgtaaccacg cgtcagcacc gtagccagaa actgaatccc gacggcaatg ccgaccatgg
    32581 tattgccata gcccagttca tgatgaacaa acagcgggat aaccggcaac ggcaacccta
    32641 cggtcatgta ggtgagaaaa accgcaaaag cgatgcggaa gagcgaaaaa ttggcagaag
    32701 atcgtgtttc ggtttggctt acagcagtca tgcattactc cagaatgcag cgcaaggcga
    32761 ggagtatccc cgtctcatct ctctggtttc agggttacgg tgcgttggca ggatttaacg
    32821 cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt tgcctggaac tggcggcgat
    32881 tgtcaatgat gtgaaaaagg gaaccatcag gttccctttt gcgttagtgc cggaggccgc
    32941 attggcgcgt aacgtcggat gcgacgctgg cgcgtctact ccgacctact gcgaattaat
    33001 ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc gaagccgaag ctgttagaca
    33061 taacggtggt cagttcgcga tcggtcgttt cggtcacgat gttcagaccc gcagcctgct
    33121 cgtccagctc ttcaatgttg atgctcgggg cgataaagcc gtgttccagc atcagcagag
    33181 agtagatagc ttcctgtacg ccagcagcgc ccagagagtg accggtcatg gctttggttg
    33241 cagaaatcgc cgggctctta tcgccgaaca cttcacggat agctgccagc tctttcacgt
    33301 cgccaaccgg agtcgaagta ccgtgggagt tcaggtaatc gattggggta tcaacgccat
    33361 gcatcgccat cttcatgcag cgtactgcgc cttcgccaga cggagcaacc atgtctgcac
    33421 catcagaggt tgcgccgtag ccaacgattt cagcatagat gtgagcacca cgcgccagcg
    33481 cgtgttccag ctcttcaacc actaccatac cgccgccgcc agcgataacg aaaccgtcac
    33541 ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc gttgtattta gtagacagcg
    33601 cacccattgc gtcgaattcg caagccattt cccagcacag ctcttcgccg ccgccagcaa
    33661 acacgatgtc ctgtttgccc agttggatct gctctactgc gttaccgata cagtgtgcgg
    33721 aagtcgcaca cgcggagctg atggagtagt taacgccatg aattttaaac ggggtggcga
    33781 ggcaggcaga aacgccggat gccatcgctt tggtgaccac atacgggcca accgctttca
    33841 ggccgcgcgg gccgcgcatt gcgtcagcgc cgaacacctg gaaacgcggg gagccgccgc
    33901 cggaacctgc aatcaggcca acgcgcgggt tattctggta agcttccgga gagaggcccg
    33961 catcagcgat tgcctgctcc atagaaagga atgcataaat ggatgcgtcg ctcataaagc
    34021 gcacaacttt gcggtcaatg aggccagtgg tatccagttt tacgttgccc cagacgtggc
    34081 tacgcatgcc ggaatccttc agctcctgag agaaagtgat ccctgaacgt ccttcacgca
    34141 gagatgccag gacttcctgc tggttattac cgatgctgga aacaatgccc aggccagtaa
    34201 tcactgcacg tttcattcaa tacctctgta agtcgcacat agagtaagtt tcgaatgcac
    34261 aatagcgtac acttgtacgc cgaacaagtc cgatcagcca tttaatagag aaatttgcgc
    34321 agccttacac acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac ttacgtgaaa
    34381 cactactcca tacaacctgc caacctcgaa tttaatgctg agggtacacc tgtttcccga
    34441 gattttgacg atgtctattt ttccaacgat aacgggctgg aagagacgcg ttacgttttt
    34501 ctgggaggta accaattaga ggtacgcttt cctgagcatc cacatcctct gtttgtggta
    34561 gcagagagcg gcttcggcac cggattaaac ttcctgacgc tatggcaggc atttgatcag
    34621 tttcgcgaag cgcatccgca agcgcaatta caacgcttac atttcattag ttttgagaaa
    34681 tttcccctca cccgtgcgga tttagcctta gcgcatcaac actggccgga actggctccg
    34741 tgggcagaac aacttcaggc gcagtggcca atgcccttgc ccggttgcca tcgtttattg
    34801 ctcgatgcag gccgcgtgac gctggattta tggtttggcg atattaacga actgaccagc
    34861 caactggacg attcgctaaa tcaaaaagta gatgcctggt ttctggacgg ctttgcgcca
    34921 gcgaaaaacc cggatatgtg gacgcaaaat ctgtttaacg ccatggcaag gttggcgcgt
    34981 ccgggcggca cgctggcgac atttacgtct gccggttttg tccgccgcgg tttgcaggac
    35041 gccggattca cgatgcaaaa acgtaagggc tttgggcgca aacgggaaat gctttgcggg
    35101 gtgatggaac agacattacc gctcccctgc tccgcgccgt ggtttaaccg cacgggcagc
    35161 agcaaacggg aagcggcgat tatcggcggt ggtattgcca gcgcgttgtt gtcgctggcg
    35221 ctattacggc gcggctggca ggtaacgctt tattgcgcgg atgaggcccc cgcactgggt
    35281 gcttccggca atcgccaggg ggcgctgtat ccgttattaa gcaaacacga tgaggcgcta
    35341 aaccgctttt tctctaatgc gtttactttt gctcgtcggt tttacgacca attacccgtt
    35401 aaatttgatc atgactggtg cggcgtcacg cagttaggct gggatgagaa aagccagcat
    35461 aaaatcgcac agatgttgtc aatggattta cccgcagaac tggctgtagc cgttgaggca
    35521 aatgcggttg aacaaattac gggcgttgcg acaaattgca gcggcattac ttatccgcaa
    35581 ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc tggaactggc gcaacagcag
    35641 ggtttgcaga tttattatca atatcagtta cagaatttat cccgtaagga tgactgttgg
    35701 ttgttgaatt ttgcaggaga tcagcaagca acacacagcg tagtggtact ggcgaacggg
    35761 catcaaatca gccgattcag ccaaacgtcg actctcccgg tgtattcggt tgccgggcag
    35821 gtcagccata ttccgacaac gccggaattg gcagagctga agcaggtgct gtgctatgac
    35881 ggttatctca cgccacaaaa tccggcgaat caacatcatt gtattggtgc cagttatcat
    35941 cgcggcagcg aagatacggc gtacagtgag gacgatcagc agcagaatcg ccagcggttg
    36001 attgattgtt tcccgcaggc acagtgggca aaagaggttg atgtcagtga taaagaggcg
    36061 cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa tggtaggcaa tgttcccgat
    36121 tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc agaaagatga ggcggtaagc
    36181 gcgccggttt ttgacgatct ctttatgttt gcggctttag gttctcgcgg tttgtgttct
    36241 gccccgctgt gtgccgagat tctggcggcg cagatgagcg acgaaccgat tccgatggat
    36301 gccagtacgc tggcggcgtt aaacccgaat cggttatggg tgcggaaatt gttgaagggt
    36361 aaagcggtta aggcggggta atctgctctc gcagtgtttg ccggatgcgg cgtgaacgcc
    36421 ttatccgacc tacgtgtgac gtgtaggcct gataagacgc gataagcgtc gcatcaggca
    36481 tgctgctcca gacgccgcaa cttactgctg tgacgcctgc tgaaacaggt tttcccacat
    36541 atcggtcacc agcgcctggt cacgcggcga caattcacca gcaccaatgg ctttttccag
    36601 actctggcta acggtcgtat gcaccgcctg agcggagtgg tcatcaccac tttccagttc
    36661 tgcgatggct aatgtcaggt ggccacgcaa atacccactg gcaaacaact catcatcact
    36721 ggcatggtca accataccgt cgattaatgc cagaatgcgt gattcaaact ccgcgatcat
    36781 cttctttcct cattgtaaaa cgtggcgctg gcttcagttg agcgcttccg gccacgggaa
    36841 ctgttccgcc gtaagttccg gcgtgtgata ataattttgt agtgctttaa tgaagcgtgc
    36901 cgggcgttcg ggaatgccgt ttgccagata atccatcacc tgcgcatgta ctcgccgttg
    36961 aaataccaca cgatccggtt cgaagtcgcc ttccagattg tcgcaactga cgttaaacgg
    37021 atatcccgcc gccacacaga acaaccagtc gagcgcctgc ggcttcactt caacatcttc
    37081 aaactggctt tgcgtctggg catcgcgtcc gtccgggcaa taccagtagc cgaaatcaac
    37141 cagctcacgg cgcgcttttc cggcaataca ccagtgcgaa atctcatgaa ttgcgctggc
    37201 ataaaagcca tgagcaaaga cgattcggtt atacggtact tccgcatcag caggaagata
    37261 gatcggttcg tcgtcgcctt taatcagacg ggtattaaaa ttatcggcaa agcagctatt
    37321 aaaaatttca atcaactgct cgtagtggtg tgtactgttc attagttcat ccccaaccag
    37381 tggaggatct cctgtccgtg gctgtcgtaa agtaatttgg cactcatcac cgccgagaca
    37441 ataacaatca tcgggcggat cagcttttgt cctttgctca acaccagtct tgaacccatg
    37501 cgcgcgccga ggaactgccc caccagcatg acaaagcccg tcgcccaaat cactttgcca
    37561 ccgagaataa acagcagtaa accgccgata tttgacgttg cgttgagtaa tttggcgtga
    37621 gccgtggctt tggcgaggtt gaatccgcac agcgtaacaa aggccagtgc gtaaaacgat
    37681 ccggctgccg gaccaaagaa tccgtcataa aaaccgacgc agccaccggc aattaacgcg
    37741 aagggtaaac cgtacatccg gcgctggcga tcttcttcac ccagctttgg catcagcaag
    37801 aaatagaggc cgatacaaat taccagaatg ggcaaaatct gccgtaagac atcagcctga
    37861 acgtattgca ccagcagtgc gccgctcatt gagccgacaa aggtcatggc gatattgagt
    37921 ttctgatcgc ttaaactaac cactttgcgg cgaataaagt agatagtggc ggaaatagag
    37981 ccgccgcagg cttgcagttt attggttgcc agcgcattag cgggagacat ccccgctgcc
    38041 atcaatgccg gaatggtgag taacccaccg ccaccggcaa tcgagtcgat aaatcccgcc
    38101 agcatggcga caaaaaagag aactcccagc aacagcgggg aaaccataaa caggctatta
    38161 aacgtttcca ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc ggcaacggag
    38221 gcggtgtctt cttctcaggc tttgttgttc ccggttttgg aggttcaaac cagctttgca
    38281 gttctgcccc gcaaccatcg cctggtggcg gtaaaggttg atcttcacac tccagactat
    38341 cggcaggaca acgtaatcgt acatgcatat gcgcgcgatg ctggaaccag ggtcgcactt
    38401 tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag ttgttgttta atcgccggat
    38461 taacaaaaat gcgtgtgacg tctttgtcct gggcggcgag tttgatcaag ctgaaaattt
    38521 ctggcttcca cagcgtgggg acaacgtgtt taccgtcgcg ggaaactaag tccagtgctt
    38581 gcgggcgcaa gagctgcgcg gaggtccagc gtgttttcgg cagttgcaag aagatatcaa
    38641 catccagtcc ggtctggtgg ctggcatgac cgccgttgaa acgcccacca gcgggcatcc
    38701 ccatatcgcc aatcagcacc gtacccatgc ccagattgct cacctggcta ctcagacgct
    38761 ggataaacat cactagatcc gggtgtccga aatagcgacg ctgatcggta cgcatgacct
    38821 gataatgttc ggactgtatc ggcagcgtgt cagcgccgac gatacagcca ttagaaaaac
    38881 tgcctatcga ttgtgcgcta cccggcacag gttgggttat tttttgccac ggcgtcgctg
    38941 ccaggcaggc gctactggca agcagggcca gcagcgcaat cgcggttttg ttcatttttt
    39001 accagcgtgg aatatcagtc ttcacatcgg cattttgcgc ccgttgccgt aacaggtgat
    39061 ccattaaaac gatcgccagc atcgcttcag cgatcggcac cgcgcggatc ccgacacagg
    39121 gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc aaagcggtta atggtacgcc
    39181 ccggcacggt aatgctggag gtcggtttca gcgccatatg ggcaatgatt tgctgcccgc
    39241 tgctgatacc gccgagaatg ccgcccgcat ggttgctctg gaaaccgtct ttggtgattt
    39301 cgtcgcgatt ctggctgccg cgcagcgcca ctacgtcaaa accgtcgcca atttccacgc
    39361 ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc ggcatccagg cggtcaaaga
    39421 ccggctcacc aagtccggcg gggacaccac tggctacaac ggtgactttc gcgccgatgg
    39481 agtcgccctc ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt ttgtccgggt
    39541 ccgggcagaa aaacggattt tgttcaacca gcgaccagtc tttgatttcc agcggaatgt
    39601 cgcccatctg ggtcaggcag ccgcggattt caataccaaa tttctcggcg agatattttt
    39661 tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg ggcggaagaa cgtccaccgc
    39721 cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta atcggcatgg cctggacgga
    39781 aaacgtcctt aatcgcactg taatcctgag agcgctggtc agtgttttcg atcaacaagc
    39841 caatgctggt gccagtagta acgccttcaa aaacaccgga gagaattttg acctgatccg
    39901 gctcgcggcg ctgggtggta tagcgcgatg tcccaggacg acgacggtca aggtcatgtt
    39961 gcaggtccgc ttccgtcagc ggaatacccg gcggaacacc atcgacgatg cagccgagcg
    40021 ccagcccgtg cgattcgccg aaagtggtta cgcgaaagag ttgtccaatt gtgtttccag
    40081 ccatcacggc tccgttattg ttgtgtttgc gtgtttactt aatctttata aatcgcgaaa
    40141 tgttcacggg ccgccagcag ctgctctttg gtgagcataa acacaccatc gccgccgtta
    40201 tcaaactcca gccaggtgaa cggaacatcc ggatattgtt ccataagatg taccatgctg
    40261 ttgccgactt cacaaatcaa cacgccatca tccgcaaggt aatctgccgc gttaccgaga
    40321 atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca ggcccagttc cggctcgtga
    40381 cggtattcgt ttggcaggtc ggacatatct tccgcatcga catacggcgg gttagtgaca
    40441 atcaggtcgt actgcacttt cggcaagtcg cggaacagat cggaacgaat cggaatgacg
    40501 ttgtggatca gaccgtgttc ttcgatgttc tgttcagcaa ccgccagcgc gtctggagag
    40561 atatccaccg cgtcgacttc cgcttccggg aaggcataag cacaggcaat ggcgatgcag
    40621 ccgctaccgg tacacatatc taaaatatgc tgcggttgcc tgctgataag tccggcaaat
    40681 ttattgttga tcagttcacc aatcggcgag cgcggcacca gcacgcgttc atcgacgtaa
    40741 aattcatggc cgcagaacca cgctttgttg gtcaggtaag ccaccggaat gcgttcattg
    40801 acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc tggaggtcag acgcgcggtg
    40861 cgcatatctt ccggaatatc cagcggcagg tagagcgaag gcaacaccag ctgtacggct
    40921 tcatcccacg ggttatcggt accgtgaccg taccagatat ttgccgcgct gaagcggctc
    40981 accgaccagc gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc atcaacgaaa
    41041 attttatcca cgtattcctc cagggcatga tcgcaataat ttcggcggct agtttgccat
    41101 gaagatgacg ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg tttaaaacga
    41161 tcgattgcgc tacgagtcgg gtaaactgta ggaaaattag aaatagagac gcataaatga
    41221 aaaagaaaac aacacttagc gaggaggacc aggctctgtt tcgccagttg atggcgggga
    41281 ctcgcaagat taagcaggac acgattgtcc accgaccgca gcgtaaaaaa gtcagcgaag
    41341 tgccggtgaa gcggttgatt caggaacagg cagatgccag ccattatttt tccgatgagt
    41401 ttcagccgtt attgaatacc gaaggcccgg tgaaatatgt acgcccggat gtgagtcatt
    41461 ttgaggcgaa gaaactgcgc cgtggcgatt attcgccgga gttgtttttg gatttacacg
    41521 gtctgacgca gctgcaggcc aagcaggaac tgggggcgtt gattgccgcc tgtcgccgcg
    41581 aacatgtatt ttgcgcctgc gtgatgcatg gtcacgggaa gcatattttg aagcaacaaa
    41641 caccgctgtg gctggcacaa catccgcatg tcatggcgtt tcaccaggct ccaaaagagt
    41701 atggcggtga tgcggctttg ttggtgttga ttgaagtgga agagtggctg ccgccggagt
    41761 tgccctgaac gtttttaacc cgcttcagac tgccgagctg caaaaatgca cagtagcatt
    41821 aggcataata atataattta acgtcgcctc ccatcggagc aaaagattgc cggaggcgac
    41881 gcgacaatag aggtcacaac tcatttaaaa tatactgaaa gcgacaagat gaatctatcg
    41941 gttgcatact acttgtatct actcgcacta ctatatcgtg gagtttttta tcagtaactg
    42001 cttgctctat tggcgtcacc gtcactttag cgttgtcaca tccatacgaa gagaaagtga
    42061 ttaaaccaga aacgaaccga caatagtcac cgcgcctatc cataacactg ctatcgcata
    42121 atttattaga ttccattaaa taggtagaga cagttttttc aataaaccct cccgtagaat
    42181 gaaggctaat gccaagatta tgattgacac gattctcact ccaggaaaca tcaaatgtat
    42241 ctacaggcac agtcgggcat accgtactat tggccgttgg tgcgcgataa gaagcgccgt
    42301 agtccgtgac cacactgtat accgatgcca gccaggggca accaaaatca tctgcggcat
    42361 caatagtaat gctgaatgtc agatattgac aatttcgacc actgggcaaa ccagggcatt
    42421 gggatgcgcc tgcattacca aagccaacta tttgtataat gggagcagct ccggggccag
    42481 gccactgatc tgtcaccaga taatcattat ttctggggac agcatgcggg tcataatatt
    42541 tcgctgtata agcaccattt cttggtgaaa ttatagtaaa ataaaactcc atcgtaggat
    42601 cagattgacc gctcgcgcca tacagcacat cgacactacc aaacgtaaca cttgcctgta
    42661 cactggtact actaagccat gtaatcagca aacaaaataa tatccatatt gtcttcatat
    42721 tataaatact caaaagttgc cgtcgctgta gcagtgaaat taccgagcgt cacatctcgc
    42781 ccactttttg cctgaaccca ggtgttaaaa agcaatgtat tactgccatc actcaaaaca
    42841 aacgtcgcac cagagttatt attgatcttc actgggacac catccactgt ctcaatacca
    42901 atacccacac cttgcgcctg tgagtctgaa tcaagagcta aaaatccagg ttgtccggta
    42961 tcctctgtgc ccgtaaatgt caccttaacg ctattttgtg attgtgaaga ccccttacag
    43021 ttttctagct caaaagccat tgagacttta cctgactgca tttgtgtact cagatctctt
    43081 gagctaatcg tcggaaagcg aacttctgcc agtaatcctc caggagcgac caacgtgcat
    43141 gatttatcaa tcaaatttcc ggtgaaatga aggttgtttt tcgcgctcgt cgctgttcct
    43201 tgagctaaca acactagccc cagtaaactc cccgctgtat atctggtgta gtaggtttgg
    43261 tttcttgtca ttggtaatcc acctgtaatg tggcataggc ttcaaatacc ccttcactga
    43321 gcaccgctcc gctttttttc accagtaccg catgtagttt cggcagatta gtttttgaaa
    43381 tatttatcgc ctttccctgt tgaaaacgct gaccgttttg ctgcaattcg acccccagcc
    43441 ccaccacgtc cgtctctacc gcagagtcat taaaatcagt ttctactccc gtccaggtca
    43501 gcttcatttg tattcctgta tcggtattct cagagtcaca ttcaacgcta taaggcactt
    43561 ctcgtttata gttatcacca gagatgtcac taataatgac ctcattaaat gcaatctcaa
    43621 tcgtcttatt ttcactgacg atgcatgttg gcggcgctat caaattgcca tgaaacgtta
    43681 tatctggaga tgagctaacc accgttgcgt aacttaccag catgataaaa aatgcgatct
    43741 gtttcattga taatcaacca ccatagttgc agtagcactg aatgcagaag ataccagcga
    43801 taccccattc tgcttcacgg gtacagcttc aagcgttggt tggttatcga tgctaaatgg
    43861 tagccaggca ctgcttccta ccgtaataag cgagtcattc gtagcgtttt gaatacgaat
    43921 accaaaccca tcaatacctg tattaagcac agtttcacca ttgatgacgc ttgtaggacc
    43981 ctgcaactgc atacgtaagt catccgcacc agtaagtcgt tgcccgcagt ccagcttgta
    44041 agttactggc tgcaaatact ggctgccatc tatacgcctg atgagcacat ccccaaaatc
    44101 gacatcgctc cccgtaacgg tacatggtgg aggcccatct acctgtacac gtaaaaccat
    44161 atttactggc ttactgcctg cgtaagtcaa ttcccctacc gtagtgcaga tcagcagccc
    44221 cccaaccata gtcagtagaa tatttttcat ccgttatctc ttaacctcat cccttgagac
    44281 tttgatcttt atccacctgg catgtgttgc cgtcacagcg gaaaaagagt ggcatccgtg
    44341 cgccaaagtc gttgacgtaa gtcagcaccg gtacgctatc cattttcaca ttcagcggaa
    44401 ctgtggtttt cggctcaatg accagaggcg agaaccccgt agccggatta ccgtttttct
    44461 gcgcactggc gttgctgatg atcacgtagt acggcgttgg gttattgacg gtaaatgcct
    44521 gaccgctgcg ggtgagcgtc accttgtgct gccacggact tttcatactg accttttcca
    44581 gcgcctttgg tcgccagaac aacttgatac gcgtttgcag cgcgatttgc agggtattgg
    44641 gtttgtttga ttttggcggg atctcgcgca cgttaaagta gaacatgctt tcacgatcgg
    44701 ctggcaattt gttgatatcc ggcattccct gcacttttac ctgcccgttc atcattgaat
    44761 cgatgcgttg cactggcggc agcacagtta acggcgaggt gatcttgtta cccttttcat
    44821 cttcaatcca gctttgagcc agataaggga gtttcggatc gttattacgc agcgtcacgc
    44881 tgattgattt gtcgctttca ttaaatatca aacgggtacg atctggcgtt acagacgcca
    44941 gggcagacaa actggtagcg ctaagcagta acgccagcgt catagcaccc aatttggcag
    45001 agcaaagtaa atcagacatg gatcattcct taatcacgtt attggttagc tgaaacagaa
    45061 acagggggat cggtgggtgt cacctgttgc gtctgctcct gaatgacagg ctttatgtca
    45121 tcaggaacaa ccggcgcgac gttgccttta tgctggcatg gcagtaacaa gccgttaaac
    45181 agatcagccg gtaacgggtc cggcaggttg atatcgcaat gcgcaacacc actccagaac
    45241 acgctcatat gttcgccagg tttcacccca gccagataaa cattgccgtc atcatcgaca
    45301 aggcccactg tctgctcgtt atcatttttt acttctgcgc caaacggtgg atggctgcca
    45361 tcttgtaaac gcagcaccgc catcgctttt tgaccactga tgacggcaaa tttgcggtag
    45421 ccaatggctc cttcagttag cgtggcttgc accaccgact gggttgcttc ggcgttttcc
    45481 ggcaatttgt tgaggtcgat atacgcctga ttgcgataat aattgttaac gtcagaaacg
    45541 acggctttac caaacatatt ggtataaaca gcagccccgt taccttccac cggaacatcg
    45601 gcaacaccat cggcatcaat caacaggcgc gtcccgccca tattctgggt acggtgaagt
    45661 gcgccgccgt gggtagtaag cgttgcgccg cccagtaacg acaagcccgc agaggtgtat
    45721 tgcccttcat ggtagttcgc actgaggtca acctgcgcca gcgaaccatc atggctgtag
    45781 tagctatcaa cgctggtgtg tttgtcactg gtgccgacgt tcaactgata gtgagtcgcg
    45841 tcatcgacac ggctaaaata accgacctgg ctgctgtccg tcccactgcc atagttgccg
    45901 ttatagctaa cggtactgtt gtcgccccac ggcatactga gcgaaatgta catgccctta
    45961 tccgcccggt tgtcatactc gtagcggtag ccagtcaggg aaacgctcat attacgaatg
    46021 ctgcccatat tgaaatagtg ggagagcatg atgttgtagt ttgtctgctc ctcacgatcc
    46081 cagtaggtat ggcgggtata gttgagataa accgaaacac ccgcatcgcg gaagttctgg
    46141 ttataagtgg cggtgtacat ctctttgtcg ttgcccgtac ggaccatttc gctgtcactg
    46201 gcatccagat actcgctcat ggtcataaag ttctcttccg agaagcgata tccagcgaaa
    46261 gtgacgcggc tgttgagctg gtcaaagtct ttggaataac tcacacggaa ggagttaccg
    46321 tccagcgaac ctttgccata agcggtgtct ttatccagtt tggtatgcga atgagtaaca
    46381 tcaaacgcga ccgcgccgaa ggtagacaaa tcgcgaccga cgcccagcgc cgcagactga
    46441 tagttttcat cacccagcgc accgccatat aacgaccagc cgttagcaat cccccaggaa
    46501 gcttcagcac cagagaaaaa tcccccctcg acatggtggc cccactcttg cggacgtccc
    46561 atcatgatct tatagcgaac ctggcctggg cgagtgaggt atggcatcga ggcggtgctg
    46621 atgtcatatt cctgtacctg gccgttctgt tcttcaatgc gaacgtgcag cgtaccggag
    46681 atagaatcac caagatcctg aatacgaaac ggtccagccg gaacctgcgt ttcgtaaatc
    46741 acacgcccca tctggctgac ggtcactttt gctgtggtgt gtgccacgcc ggaaatgtct
    46801 ggcgcgtagc cgcgcaggtt gggaggcaac atttgatcgt cagtgctgac actgccacca
    46861 acataattaa aaccgtcgaa aatatcggaa ttgaggtaat cttcgcccag cgccagtttg
    46921 gcttttaatg acggtaacgc ccgccaggca taatagcgac tccactccca ttttttttgt
    46981 gtgtcatcgc cgctaaattc ctcgtcatca tcattactgc gggtatgttg atagttggtc
    47041 tgccagtcag cgcgcatacg ccatggcccc aggttaaccc cgaccgtccc gttgccgctg
    47101 atctcgttac tgtcatcgcc gccattttct tcgtgtcgtg tttgcgcagt gatgctgtag
    47161 tccgcaatga tcccggagat gccatcatcc caacgagaag gtggatccca gtcgggccag
    47221 gtatattcga ggtaagcctg cggtagtgaa atgactaatg cggactggct taaatcagcc
    47281 ttaatttcca tgccttccag ttgaccgggc ttcaggcatt taccatcgtg gctccattgc
    47341 agatttttcg ccacgtcttc ttttaaacca aactgcgcca ccagttccgg tgtcagacaa
    47401 gcatagcttt tgctaacgtc atcttcacca gcataccagt aaatatcgta ctcttccgcc
    47461 aatggctgtt tatttagttg aacctgtaaa ttgtattttc cgggctcaac atatccctgg
    47521 ctggaaaaac gcttcagatc aatttttgtg tcgcctttta attccagaaa acgggaatca
    47581 aactgaatgt cgtcttcagc ccagacactg ctataactcc ctgacattgc cagcgcaatg
    47641 caccacggaa gaatccgtaa tcgaaaaaga gaatggtcag gcatacacgt ttctatccgt
    47701 ttttattctt gttcggtcaa aatccattta aatcccccat agcagtgtcg ctatgggggt
    47761 atgccttaca ggtaagtaat ctggaaggtg gtatttgctt cgaaattacc cagatctggg
    47821 gcatcagctg cgccgaccag ccaggcttta aagttcagtg tctgcttcgc tttgccttta
    47881 ttggtagaag tgtctttcac tattttctgg tcaataccca tgccgctttt gtaagaggta
    47941 cccagagagt caccgatcgc caggctaaca ttgttaaatg ccgcaccggt atcggtattg
    48001 aaaatggtgt aataattgcc gctatttgca gaagaaacgg taccagtaaa ggtagtggtc
    48061 attgtttcct gagtatcaaa tacgcaatct tgcagacgaa tctggaaact tttcggcgtg
    48121 gtggtgccgt tatttttcag tttttcggca ccgatatcac ccatatcaac ttccagtttg
    48181 tgatcgtccg ggacgataga acacactgag tcttcaatgg tgccgtaaaa acgtgcagta
    48241 ccgttgttac ccgcagcgat tgttgctgta gaggtgaacg cgcccatcac catt
//
LOCUS       DAZFZC010000029        48204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFZC010000029 DAZFZC010000000
VERSION     DAZFZC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.029"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            335..1651
                     /gene="fucP"
                     /locus_tag="ACHKXP_001888"
     CDS             335..1651
                     /gene="fucP"
                     /locus_tag="ACHKXP_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7646909.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1684..3459
                     /gene="fucI"
                     /locus_tag="ACHKXP_001889"
     CDS             1684..3459
                     /gene="fucI"
                     /locus_tag="ACHKXP_001889"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO7646910.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3568..4986
                     /gene="fucK"
                     /locus_tag="ACHKXP_001890"
     CDS             3568..4986
                     /gene="fucK"
                     /locus_tag="ACHKXP_001890"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO7646911.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4988..5410
                     /gene="fucU"
                     /locus_tag="ACHKXP_001891"
     CDS             4988..5410
                     /gene="fucU"
                     /locus_tag="ACHKXP_001891"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO7646912.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5468..6199
                     /gene="fucR"
                     /locus_tag="ACHKXP_001892"
     CDS             5468..6199
                     /gene="fucR"
                     /locus_tag="ACHKXP_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO7646913.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVIA"
     gene            complement(6243..7343)
                     /gene="rlmM"
                     /locus_tag="ACHKXP_001893"
     CDS             complement(6243..7343)
                     /gene="rlmM"
                     /locus_tag="ACHKXP_001893"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO7646914.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWGYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7336..7731)
                     /gene="ygdD"
                     /locus_tag="ACHKXP_001894"
     CDS             complement(7336..7731)
                     /gene="ygdD"
                     /locus_tag="ACHKXP_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO7646915.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7750..8667)
                     /gene="gcvA"
                     /locus_tag="ACHKXP_001895"
     CDS             complement(7750..8667)
                     /gene="gcvA"
                     /locus_tag="ACHKXP_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO7646916.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(9018..9245)
                     /gene="ygdI"
                     /locus_tag="ACHKXP_001896"
     CDS             complement(9018..9245)
                     /gene="ygdI"
                     /locus_tag="ACHKXP_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO7646917.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9437..10642
                     /gene="csdA"
                     /locus_tag="ACHKXP_001897"
     CDS             9437..10642
                     /gene="csdA"
                     /locus_tag="ACHKXP_001897"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO7646918.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10642..11085
                     /gene="csdE"
                     /locus_tag="ACHKXP_001898"
     CDS             10642..11085
                     /gene="csdE"
                     /locus_tag="ACHKXP_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO7646919.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11136..11942)
                     /gene="tcdA"
                     /locus_tag="ACHKXP_001899"
     CDS             complement(11136..11942)
                     /gene="tcdA"
                     /locus_tag="ACHKXP_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO7646920.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12181..13278)
                     /gene="mltA"
                     /locus_tag="ACHKXP_001900"
     CDS             complement(12181..13278)
                     /gene="mltA"
                     /locus_tag="ACHKXP_001900"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO7646921.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13487..13563
                     /locus_tag="ACHKXP_001901"
     tRNA            13487..13563
                     /locus_tag="ACHKXP_001901"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13521..13523,aa:Met,seq:cat)
     gene            complement(13637..14890)
                     /gene="amiC"
                     /locus_tag="ACHKXP_001902"
     CDS             complement(13637..14890)
                     /gene="amiC"
                     /locus_tag="ACHKXP_001902"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO7646922.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGVTLARRG"
     gene            15122..16453
                     /gene="argA"
                     /locus_tag="ACHKXP_001903"
     CDS             15122..16453
                     /gene="argA"
                     /locus_tag="ACHKXP_001903"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO7646923.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16515..18341)
                     /gene="recD"
                     /locus_tag="ACHKXP_001904"
     CDS             complement(16515..18341)
                     /gene="recD"
                     /locus_tag="ACHKXP_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO7646924.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLSDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18341..21883)
                     /gene="recB"
                     /locus_tag="ACHKXP_001905"
     CDS             complement(18341..21883)
                     /gene="recB"
                     /locus_tag="ACHKXP_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO7646925.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIERRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQEQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(21876..24764)
                     /gene="ptrA"
                     /locus_tag="ACHKXP_001906"
     CDS             complement(21876..24764)
                     /gene="ptrA"
                     /locus_tag="ACHKXP_001906"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO7646926.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24940..28308)
                     /gene="recC"
                     /locus_tag="ACHKXP_001907"
     CDS             complement(24940..28308)
                     /gene="recC"
                     /locus_tag="ACHKXP_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO7646927.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAHWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDATLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28321..28644)
                     /gene="ppdC"
                     /locus_tag="ACHKXP_001908"
     CDS             complement(28321..28644)
                     /gene="ppdC"
                     /locus_tag="ACHKXP_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO7646928.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(28629..29036)
                     /gene="ygdB"
                     /locus_tag="ACHKXP_001909"
     CDS             complement(28629..29036)
                     /gene="ygdB"
                     /locus_tag="ACHKXP_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO7646929.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(29033..29596)
                     /gene="ppdB"
                     /locus_tag="ACHKXP_001910"
     CDS             complement(29033..29596)
                     /gene="ppdB"
                     /locus_tag="ACHKXP_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7646930.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTGTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(29587..30057)
                     /gene="ppdA"
                     /locus_tag="ACHKXP_001911"
     CDS             complement(29587..30057)
                     /gene="ppdA"
                     /locus_tag="ACHKXP_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7646931.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30242..31036)
                     /gene="thyA"
                     /locus_tag="ACHKXP_001912"
     CDS             complement(30242..31036)
                     /gene="thyA"
                     /locus_tag="ACHKXP_001912"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO7646932.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(31043..31918)
                     /gene="lgt"
                     /locus_tag="ACHKXP_001913"
     CDS             complement(31043..31918)
                     /gene="lgt"
                     /locus_tag="ACHKXP_001913"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO7646933.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32069..34315)
                     /gene="ptsP"
                     /locus_tag="ACHKXP_001914"
     CDS             complement(32069..34315)
                     /gene="ptsP"
                     /locus_tag="ACHKXP_001914"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7646934.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34328..34858)
                     /gene="rppH"
                     /locus_tag="ACHKXP_001915"
     CDS             complement(34328..34858)
                     /gene="rppH"
                     /locus_tag="ACHKXP_001915"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO7646935.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35211..35357)
                     /gene="ygdT"
                     /locus_tag="ACHKXP_001916"
     CDS             complement(35211..35357)
                     /gene="ygdT"
                     /locus_tag="ACHKXP_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO7646936.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35543..36232
                     /gene="mutH"
                     /locus_tag="ACHKXP_001917"
     CDS             35543..36232
                     /gene="mutH"
                     /locus_tag="ACHKXP_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO7646937.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALVGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36301..37014
                     /gene="ygdQ"
                     /locus_tag="ACHKXP_001918"
     CDS             36301..37014
                     /gene="ygdQ"
                     /locus_tag="ACHKXP_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7646938.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37152..37370
                     /gene="ygdR"
                     /locus_tag="ACHKXP_001919"
     CDS             37152..37370
                     /gene="ygdR"
                     /locus_tag="ACHKXP_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO7646939.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37478..38518
                     /gene="tas"
                     /locus_tag="ACHKXP_001920"
     CDS             37478..38518
                     /gene="tas"
                     /locus_tag="ACHKXP_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO7646940.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGIELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38550..39743)
                     /gene="lplT"
                     /locus_tag="ACHKXP_001921"
     CDS             complement(38550..39743)
                     /gene="lplT"
                     /locus_tag="ACHKXP_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO7646941.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39736..41895)
                     /gene="aas"
                     /locus_tag="ACHKXP_001922"
     CDS             complement(39736..41895)
                     /gene="aas"
                     /locus_tag="ACHKXP_001922"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO7646942.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEGGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            42481..43512
                     /gene="galR"
                     /locus_tag="ACHKXP_001923"
     CDS             42481..43512
                     /gene="galR"
                     /locus_tag="ACHKXP_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO7646943.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43519..44781)
                     /gene="lysA"
                     /locus_tag="ACHKXP_001924"
     CDS             complement(43519..44781)
                     /gene="lysA"
                     /locus_tag="ACHKXP_001924"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO7646944.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44904..45839
                     /gene="lysR"
                     /locus_tag="ACHKXP_001925"
     CDS             44904..45839
                     /gene="lysR"
                     /locus_tag="ACHKXP_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO7646945.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPEDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45826..46518)
                     /gene="ygeA"
                     /locus_tag="ACHKXP_001926"
     CDS             complement(45826..46518)
                     /gene="ygeA"
                     /locus_tag="ACHKXP_001926"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO7646946.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAAFGLQRAGAEGIVLCTNTMHKVADVIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46647..48065)
                     /gene="araE"
                     /locus_tag="ACHKXP_001927"
     CDS             complement(46647..48065)
                     /gene="araE"
                     /locus_tag="ACHKXP_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO7646947.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        11793 a        13100 c        11941 g        11370 t
ORIGIN      
        1 tcaaaacggt catttttcta ttcctccaag cccggaatga ccgttttcgg cacaaacaat
       61 taatacggtc atctgatttg tgttttttat gatttatttt ctgaaacggg catgaaattt
      121 cgattattaa agtgatggta gtcacataaa gtcaccttcc agctaataag tgtgaccgcc
      181 gtcatattac agagcgtttt ttatttgaaa atgaatccat gagttcattt cagacaggca
      241 aatattcact gatatgaagc ccgaactcgc tggttttgca cttttgaaaa cataaccgat
      301 tacgtgctta agcttctgaa cctaagagga tgctatggga aacacatcaa tacaaacgca
      361 gagttaccgt gcggtagata aagatgcagg gcaaagcaga agttacatta ttccattcgc
      421 gctgctgtgc tcactgtttt ttctttgggc ggtagccaat aaccttaacg acattttatt
      481 acctcaattc cagcaggctt ttacgctgac aaatttccag gctggcctga tccaatcggc
      541 cttttacttt ggttatttca ttatcccaat ccctgctggg atattgatga aaaaactcag
      601 ttataaagca gggattatta ccgggttatt tttatatgcc ttgggtgctg cattattctg
      661 gcccgccgca gaaataatga actacacctt atttttagtt ggcctattta ttattgcagc
      721 cggattaggt tgtctggaaa ctgccgcaaa cccttttgtt acggtattag ggccggaaag
      781 tagtggtcac ttccgcttaa atcttgcgca aacatttaac tcgtttggcg caattatcgc
      841 ggttgtcttt gggcaaagtc ttattttgtc taacgtgcca catcaatcgc aagacgttct
      901 cgataaaatg tctccagagc aattgagtgc gtataaacac agcctggtat tatcggtaca
      961 gacaccttat atgatcatcg tggctatcgt gttactggtc gccctgctga tcatgctgac
     1021 gaaattcccg gcattgcaga gtgataatca cagtgacgcc aaacaaggat cgttctccgc
     1081 atcgctttct cgcctggcgc gtattcgcca ctggcgctgg gcggtattag cgcaattctg
     1141 ctatgtcggc gcacaaacgg cctgctggag ctatttgatt cgctacgctg tagaagaaat
     1201 tccaggtatg actgcaggct ttgccgctaa ctatttaacc ggaaccatgg tgtgcttctt
     1261 tattggtcgt ttcaccggta cctggctcat cagtcgcttc gcaccacaca aagtcctggc
     1321 cgcctacgca ttaatcgcta tggcactgtg cctgatctca gccttcgctg gcggtcatgt
     1381 gggcttaata gccctgactt tatgcagcgc ctttatgtcg attcagtacc caacaatctt
     1441 ctcgctgggc attaagaatc tcggccagga caccaaatat ggttcgtcct tcatcgttat
     1501 gaccattatt ggcggcggta ttgtcactcc ggtcatgggt tttgtcagtg acgcggcggg
     1561 caacatcccc actgctgaac tgatccccgc actctgcttc gcggtcatct ttatctttgc
     1621 ccgtttccgt tctcaaacgg caactaactg aacatatttt ccgaataaag tgaggaatct
     1681 gtaatgaaaa aaatcagctt accgaaaatt ggtatccgcc cggttattga cggtcgtcgc
     1741 atgggtgttc gtgagtcgct tgaagaacaa acaatgaata tggcgaaagc tacggccgca
     1801 ctgctgaccg agaaactgcg ccatgcctgc ggagctgccg tcgagtgtgt catttccgat
     1861 acctgtatcg cgggtatggc tgaagccgct gcttgcgaag aaaaattcag cagtcagaat
     1921 gtaggcctca ccattacggt aacgccttgc tggtgctatg gcagtgaaac catcgacatg
     1981 gatccaaccc gcccgaaggc catttggggc tttaacggca ctgaacgccc cggcgctgtt
     2041 tacctggcag cggctctggc agctcacagc cagaaaggca tcccagcatt ctccatttac
     2101 ggtcatgacg ttcaggatgc cgatgacaca tcgattcctg ccgatgttga agaaaaactg
     2161 ctgcgctttg cccgcgccgg tttggccgtc gccagcatga aaggtaaaag ctatctgtcg
     2221 ctgggcggcg tttcgatggg tatcgccggt tccattgttg atcacaactt ctttgaatcc
     2281 tggctgggaa tgaaagtcca ggcggtggat atgaccgaac tgcgtcgccg tatcgatcag
     2341 aagatttacg acgaagccga attggaaatg gcactggcct gggctgataa aaacttccgc
     2401 tatggcgaag atgaaaataa caaacagtat caacgtaatg ccgagcaaag ccgcgcagtt
     2461 ctgcgcgaaa gtttactgat ggcgatgtgt atccgcgaca tgatgcaagg caacagcaaa
     2521 ctggccgata ttggtcgcgt ggaagaatca cttggctaca acgccatcgc tgcgggcttc
     2581 caggggcaac gtcactggac cgatcaatat cccaatggtg acaccgccga agcgatcctc
     2641 aacagttcat ttgactggaa tggcgtgcgc gaaccctttg tcgtggcgac cgaaaacgac
     2701 agtcttaacg gcgtggcaat gctaatgggt caccagctca ccggcaccgc tcaggtattt
     2761 gccgatgtgc gtacctactg gtcaccagaa gcaattgagc gtgtaacggg gcataaactc
     2821 gatggactgg cagaacacgg catcatccat ttgatcaact caggttctgc tgcgctggac
     2881 ggttcctgta aacaacgcga cagcgaaggt aacccgacga tgaagccaca ctgggaaatc
     2941 tctcagcaag aggctgacgc ttgcctcgcc gctaccgaat ggtgcccggc gatccacgaa
     3001 tacttccgtg gcggcggtta ctcttcccgc ttccttaccg aaggcggcgt cccgttcacc
     3061 atgactcgtg tcaacatcat caaaggcctg ggaccggtac tgcaaatcgc ggaaggctgg
     3121 agcgtggaat tgccgaagga tgtgcatgac atcctcaaca aacgcaccaa ctcaacctgg
     3181 ccaaccacct ggtttgcacc gcgcctcacc ggtaaagggc cgtttacgga tgtgtactcg
     3241 gtaatggcga actggggcgc taaccatggg gttctgacca tcggccacgt tggcgcagac
     3301 tttatcactc tcgcctccat gctgcgtatc ccggtatgta tgcacaacgt tgaagagacc
     3361 aaagtgtatc gtccttctgc ctgggctgcg cacggcatgg atattgaagg ccaggattac
     3421 cgcgcttgcc agaactacgg tccgttgtac aagcgttaat acattactcc ctgatgtgat
     3481 gcccggtcgc tccggctacc gggcctgaac aagcaagagt ggttagccgg ataagcaatg
     3541 ttatccggct atattgcagg agcgattatg aaacaagaag ttatcctggt actcgactgt
     3601 ggcgcgacca atgtcagggc catcgcggtt aatcggcagg gcaaaattgt tgcccgcgcc
     3661 tcaacgccta atgccagcga tatcgcgatg gaaaacaaca cctggcacca gtggtcttta
     3721 gacgccattt tgcaacgctt tgctgattgc tgtcggcaaa tcaatagtga actgactgaa
     3781 tgccacatcc gcggtatcgc cgtcaccacc tttggtgtgg atggcgctct ggtagataag
     3841 caaggcaatc tgctctatcc gatcattagc tggaaatgtc cgcgaacagc agcggttatg
     3901 gacaatattg aacagttgat ctccgcacag cggttgcagg ctatttctgg cgtcggagcc
     3961 tttagtttca atacgttata taagttggtg tggttgaaag aaaatcatcc acaactgctg
     4021 gaacgcgcgc acgcctggct cttcatttcg tcgctgatta accaccgttt aaccggcgaa
     4081 ttcactactg atatcacgat ggccggaacc agccagatgc tggatatcca gcaacgcgat
     4141 ttcagtccgc aaattttaca agccaccggt attccacgcc gactcttccc tcgtctggtg
     4201 gaagcgggtg aacagattgg tacgctacag aacagcgccg cagcaatgct cggcttaccc
     4261 gttggcatac cggtgatttc cgcaggtcac gatacccagt tcgccctttt tggcgcaggt
     4321 gccgaacaaa atgaacccgt gctctcctcc ggtacatggg aaattttaat ggttcgcagc
     4381 gcccaggttg atacttcgct gttaagtcag tacgccggtt ccacctgcga actggatagc
     4441 caggcagggt tgtataaccc aggtatgcaa tggctggcat ccggcgtgct ggaatgggtg
     4501 agaaaactgt tctggacggc tgaaacaccc tggcaaatgt tgattgaaga ggctcgtctg
     4561 atcgcgcctg gcgcggatgg cgtgaaaatg cagtgtgatt tattgtcgtg tcagaacgct
     4621 ggctggcaag gagtgacgct taatactacg cgggggcatt tctatcgcgc ggcgctggaa
     4681 gggttaactg cgcaattaca gcgcaatcta cagatgctgg aaaaaatcgg gcactttaaa
     4741 gcctctgaat tattgttagt cggcggagga agtcgcaaca cattgtggaa tcagattaaa
     4801 gccaatatgc ttgatattcc ggtaaaagtt ctcgacgatg ccgaaacgac cgtcgcagga
     4861 gctgcgctgt tcggttggta tggcgtaggg gaatttaaca gcccggaaga agcccgtgca
     4921 cagattcatt atcagtaccg ttatttctac ccgcaaactg aacctgaatt tatagaggaa
     4981 gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc gaactattga aagtgctggc
     5041 agagatggga catggagatg aaattatttt ttccgatgct cactttcccg cccattcgat
     5101 gggaccgcag gtgatccgcg ctgatggcct gttggtgagc gacttgctcc aggcgattat
     5161 cccgttattt gaactggaca gttacgcacc gccgctggtg atgatggcag cggtagaagg
     5221 tgacactctc gatcctgaag tagaacgacg ttaccgtaat gcgctttcac tacaagcccc
     5281 gtgtcctgac atcatccgca tcaatcgttt tgcgttttat gaacgggcgc aaaaagcctt
     5341 tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat attcttttaa aaaaaggggt
     5401 aacaccgtaa tctcataccg gtacgcccgc atgacgcggg cggttatcga atgatggggt
     5461 gaaaaatatg aaagcggcac gccagcaagc gatagtcgac ctgctgctga accataccag
     5521 cctgaccacg gaagctctct ctgaacagct aaaggtcagt aaagaaacca ttcgtcgcga
     5581 tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat catggacgcg ctaaatatat
     5641 ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc aggctgaaaa gccattatgc
     5701 gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt gaagaaggga tggtgatagc
     5761 cttagacgcc agttcaactt gctggtatct ggcacgccag ttgcctgaca tcaacattca
     5821 ggtcttcacc aatagccatc cgatttgcca tgaactcggt aaacgcgaac gcattcaact
     5881 gatcagttcc ggcggcacac ttgagcgcaa atatggctgt tacgtcaatc cctcgctgat
     5941 ttcccaactt aaatcgctgg aaatcgatct gtttattttt tcttgtgaag ggatcgatag
     6001 cagcggcgca ctgtgggact ccaatgccat caacgctgat tacaaatcga tgctattaaa
     6061 acgtgccgcg caatcgttgt tattgattga taaaagtaaa tttaatcgtt caggggaagc
     6121 ccgcatcggg catctggatg aggtaacgca cattatttct gatgagcgcc aggttgcaac
     6181 ttctttggta atagcctgac ggaaagggta gcaggccgga gacgacgccc cggccttgcc
     6241 tgttatcgct cgtcgcgacg accacccacc gcagcccaga tgcggcggac gtgcaccgtc
     6301 acttcttcgc gatcgtgata caactgccgc gcctgaatct gagcatttat gccatgttca
     6361 tcaagctgtg cctgaatata cgccagattg tgtgacactt cttcgtagcg ttttttcatc
     6421 ggcagtttga ggttgaaaat ggtttcacga caccagccat taaccagcca ctgcgccatc
     6481 aatgccgcaa ctttcgccgg tttttcaacc atatcgcata ccatccagga gatattgctg
     6541 cgcgtcggac ggaatttgaa accgtcttcc cgcagccacg tcacctgtcc ggtatccatc
     6601 agactttgcg ccatcgggcc gttgtcgacg gaataacccc acatgttgcg cttcaccagt
     6661 tggtaggtcc agccgccagg gcaagcgcct aaatccaccg cccacatccc gttcgccagg
     6721 cgttcatccc actcatccgc aggaataaac acatgaaatg cctcttccag tttgagtgtg
     6781 gaacgactcg gcgcatctgc cggaaatttc aggcgcggaa tgcccatata gaacggcgaa
     6841 ttattgttgc tgtatgagta accggtatag cagcagcctg gtgcaataaa gaatacatgc
     6901 acaaccggac gcttcggcgt ttcatagttc gccagcaccc cagcatcgcg caaggcagcg
     6961 cgtagcggca cggtgaattt acggcagaat ttcagcaact ctttgctttc gttggtatcg
     7021 gcaacttcaa cacgcagttc accgcccttc tctactacac cctgtaacat gccgacaatg
     7081 ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt cccccaccac aaaccactgg
     7141 cgggcaaaaa ttaatgaact gaacggcagc tcacggatta acttatcgcc atcatcaggt
     7201 tgataacatt cataaatgac ataacccgca ttctctttca cgcgggcaaa accgaaaatt
     7261 tcccgctggc cggctttatc ggtaatttct gcggcgcact ctttttcaaa ccccggacgg
     7321 cacagcaata caaccttatt catgacttac gcccttgcgc tttaaacgga tagcaccaac
     7381 taacattaac gcccagcccg caaggaagct cacgccgcca accggagtga caaacgccca
     7441 caaacgcaga tgggacagtg ccaggcaata aaggctgccg ctgaacagca ccgtgcctaa
     7501 cgcgaggaaa acgctactcc agtaaaacca gatactgatg cgacgctgca ttgccaccgc
     7561 cagacctaag atcgccagcg tatgaaacgc ctggtattcg agaccggtct ggatccagcc
     7621 catctcaacg gcccccatgg ttttacttaa cacatgcgcg ccaaaagcgc ccagagccac
     7681 aaaaatgaag ccgctaatgg cggcgaaaat cagcataaaa cggctggtca tggtcgtacc
     7741 ctacgtaaat tattgttcat aacgaaagcg gaatttttct tgttcagcag cggctttcgc
     7801 caggatccat tgacgaaagg cggctatttt acccagttct gcctggctgt catgacaaac
     7861 cagataaaaa gcgtttttac tgaccagaac atcattaaac gggcaaacaa gacgtccggc
     7921 ctcgatttca gattgcgcca tcacgttatt tgccagcgcc actccctgcc cgtggatagc
     7981 cgcttgcagc accatggcgc tatggctaaa aattggccct tgctgaacgt tgatatgatt
     8041 taaccccaac tgtcgggtat atgtctgcca gtcacggcgc gacgcatcat gtaataacgt
     8101 atgtttagcc agatcttccg gggtcttcaa gggtttttcg ccagtcagca gtagcggcga
     8161 acacaccggc aataaatatt cggcgtacag tttttccacc cgtagccccg gccagttgcc
     8221 ccgaccataa aatatcgcca catcaacatc atccgccagc ttatcttcct gacgatcaac
     8281 cgcctggatt cgaacgtcaa ttcccggata agctgaatta aagctggaaa gtcgcggaac
     8341 caaccaatga atggcgaaac tggggagtaa actgaccgtc aacgccccct tggcgctacg
     8401 ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat atctctttga tatcgaggaa
     8461 atagctttgc ccttcctcgg tcagcaggag tgaacgatta cggcggcgga acagttttag
     8521 ccccaaaaaa tcctcaagag acttgatttg atgacttact gcggcctggg tcacaaaaag
     8581 ctcttctgct gcgcgagtga aacttaaatg gcgtgctgca gcatcaaaaa ctcgtaaggc
     8641 atttagcggt ggtaatcgtt tagacatggc ttttaaactt tgatgttaaa tgaatttaac
     8701 aattagatca cactgtgtaa cctattagtt tttttaatct gagccattat aaattgtccg
     8761 ttgagcttct accagcaaat acctatagtg gcggcacttc ctgagccgga acgaaaagtt
     8821 ttatcggaat gcgtgttctg gtgaactttt ggcttacggt tgtgatgttg tgttgttgtg
     8881 tttgcaattg gtctgcgatt cagaccatgg tagcaaagct accttttttc acttcctgta
     8941 catttaccct gtctgtccat agtgattaat gtagcaccgc ctaattgcgg tgcttttttt
     9001 taccttgcga tcgcgaatta ctgatccagt tcgaccatct ctttcacgtc agtacggttg
     9061 atctgctgtt tgttgccatt agcgtcttta tacgaaatca taccggtatc gttatcagtc
     9121 tgtggtttgc cgtcagagac gatggtacgt ccgtcattgg tgtgcatcac atagttcgaa
     9181 ccggaacagg cgctcagggc aaaagtcagc atacaggcag aaataattgc ggcagtcttt
     9241 ttcatcatct tctccttaaa gcgattaata aatcttaata gtattgatta acaggctaaa
     9301 attaacgcct aacactattc agcatatgtt actgacgcgg cttcgccagg atatccagat
     9361 aattctgatg gttagcactc tccttgtatc aaagtgaatt ttgcgtcacg atcggtgcat
     9421 caagccgagg agtaccatga acgtttttaa tcccgcgcag tttcgcgccc agtttcccgc
     9481 actacaggat gcgggcgtct atctcgacag cgccgcgacc gcgcttaaac ctgaagccgt
     9541 ggttgaagcc acccaacagt tttacagtct gagcgccgga aacgtccatc gcagccagtt
     9601 tgccgaagcc caacgcctga ccgcgcgtta tgaagctgca cgagagaaag tggcgcaatt
     9661 actgaatgca ccggatgata aaactatcgt ctggacgcgc ggcaccactg aatccatcaa
     9721 catggtggca caatgctatg cgcgtccgcg tctgcaaccg ggcgatgaga ttattgtcag
     9781 cgtggcagaa caccacgcca acctcgtccc ctggctgatg gtcgcccaac aaactggagc
     9841 caaagtggtg aaattgccgc ttaatgcgca gcgactgccg gatgtcgatt tgttgccaga
     9901 actgattact ccccgtagtc ggattctggc gttgggtcag atgtcgaacg ttactggcgg
     9961 ttgcccggat ctggcgcgag cgattacctt tgctcattca gccgggatgg tggtgatggt
    10021 tgatggtgct cagggggcag tgcatttccc cgcggatgtt cagcaactgg atattgattt
    10081 ctatgctttt tcaggtcaca aactgtatgg cccgacaggt atcggcgtgc tgtatggtaa
    10141 atcagaactg ctggaggcga tgtcgccctg gctgggcggc ggcaaaatgg ttcacgaagt
    10201 gagttttgac ggcttcacga ctcaatctgc gccgtggaaa ctggaagctg gaacgccaaa
    10261 tgtcgctggt gtcataggat taagcgcggc gctggaatgg ctggcagatt acgatatcaa
    10321 ccaggccgaa agctggagcc gtagcttagc aacgctggcg gaagatgcgc tggcgaaacg
    10381 tcccggcttt cgttcattcc gctgccagga ttccagcctg ctggcctttg attttgctgg
    10441 cgttcatcat agcgatatgg tgacgctgct ggcggagtac ggtattgccc tgcgggccgg
    10501 gcagcattgc gctcagccgc tactggcaga attaggcgta accggcacac tgcgcgcctc
    10561 ttttgcgcca tataatacaa agagtgatgt ggatgcgctg gtgaatgccg ttgaccgcgc
    10621 gctggaatta ttggtggatt aatgacaaac ccgcaattcg ccggacatcc gttcggcaca
    10681 accgtaaccg cagaaacgtt acgcaatacc ttcgcaccgt tgacgcaatg ggaagataaa
    10741 tatcgccagt tgatcatgct ggggaaacag cttccggcat tgccagacga gttaaaagcg
    10801 caggctaaag agattgccgg atgcgaaaac cgcgtctggc tgggatatac agtggctgaa
    10861 aacggcaaaa tgcatttctt tggcgacagc gaagggcgca ttgtgcgcgg cctgctggcg
    10921 gtgttgttga ctgccgttga ggggaaaacc gccgccgagt tgcaggcaca gtcaccactg
    10981 gcattgtttg atgagctggg attacgtgcg cagcttagcg cctcacgcag ccaggggtta
    11041 aatgcgttaa gcgaggcgat tatcgctgcg acgaagcagg tttaagcaca gaaccgggtc
    11101 ggataagacg ttcgcgtctc atccgacctg attgtttaac cctgacgcgc cgctttcgcc
    11161 atcatcttct tcagcgcatg agaaaccgca acaaacccaa aggtggcggt caccatcgtt
    11221 gccgcgccaa atccagatgc acaatccatc cgcttcggcc cttctgccgt ggctttcatc
    11281 gcacacaccg taccgtctga ctgcgggtac accagcgctt cagtagaaaa cacgcaatcc
    11341 acgccgagct tacctttact gtttttcact acgccaaaat cgcttttcag gcgctcgcgc
    11401 aacttcgccg ccagcgggtc ctgaatcgtt ttcgccagat cggtaacctg aatctgcgtc
    11461 ggatcaatct gcccacccgc gccaccggtt gtgaccagtg ggattttatt gcgccgacaa
    11521 taagcaatca gcgccgcttt gggccgtaca ctatcaatgg catcaatcac gtacgaataa
    11581 cccacgctca tatactgcgc tacgttatcc ggcgtcacga aatcatccac caccgttaca
    11641 cggcactccg ggttaatctg gcgaatacgc tccgccataa cttccgcttt tgccagccca
    11701 acgttatcgc gcagggcatg aatttgccga ttggtattgg tgacgcacac atcatccata
    11761 tcgataagcg tgattgcgcc aatccccgtg cgcgccagcg cttccgccgc ccaggaaccg
    11821 acaccaccga taccaaccac acaaatatgc gcgtcagcaa acagttgcaa cgctttttca
    11881 ccatacagac gcgctgtgcc accaaaacgc tgacgccatg catcactaat taccacagac
    11941 atagcacctc agaattaaaa agggcgaggt tatccctcac cctaaacgca taaatgcctg
    12001 atgcgctacg cttatcaggc ctacataact cctgcaatat tttggatttg catgatttgt
    12061 aggccggata aggcgttcac gccgcatccg gcatgaacaa cgcgcactta gtcaataatc
    12121 aggatgtgag gttacccctc accctgtcat atccgtaaaa acggcataca gaatatcaca
    12181 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    12241 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    12301 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    12361 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    12421 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    12481 ccctttcacc ggagcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    12541 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    12601 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    12661 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    12721 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    12781 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    12841 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    12901 aattaccggc gtgtaataac cggtaaactg cacgttacca tagttgtcgg caccttccat
    12961 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    13021 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    13081 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    13141 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    13201 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    13261 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    13321 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    13381 ataggcttta gtgatttgtt tttgttcaaa atcatgccaa atccgtgatc ggggtaaaaa
    13441 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggacgcg gggtggagca
    13501 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    13561 ccaattattg aacaccctaa cgggtgtttt tttgtttctg gtctcccata aaaaagcgcc
    13621 attcagcgcc tttttatcat ccccttctcg ccagcgtcac cccatcggca aaatacgctt
    13681 taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac
    13741 gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg
    13801 cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac
    13861 cgagcttatt cagtaccgct ttaccaaact tcaggctgtc ggcaatggtc agcgactgta
    13921 ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg ccaccaatca
    13981 agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt gcacctttgg
    14041 ttgagagcgc aaacacagag gaaccgctcg gctgacgact ggtaaaggcg tcggcatgga
    14101 tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact tgcaatggaa
    14161 tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag
    14221 agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg tatttcccca
    14281 ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc
    14341 gccctgcttt acccggttgt ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc
    14401 ctttgttgta atcctccagc agcgccagca gcgggtcctg catatcctgt gcattggccg
    14461 gatagaggtc catcaccaga cgctctttaa acccggcgac cggcgcaagg gcaaacagct
    14521 gcggttttac gttttgcttt aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc
    14581 cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga
    14641 gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttccggg ttactcaacg
    14701 cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg
    14761 caggccagac gcgcaccgcc acgacctggc tgaccgcagc cagactgacc tgacttacgc
    14821 tcaatagcca catggcaccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg
    14881 atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgactaa
    14941 tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg gtaaacattc
    15001 tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt tacacttgca
    15061 ccctgcgaaa aaacagaata aaaatacaat aatttcgaat aatcatgcaa agaggtgtgc
    15121 cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat
    15181 caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga
    15241 gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt
    15301 ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc accacgaacc
    15361 gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc
    15421 tgcgggaaca ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacgcc
    15481 gctgcagggc gcgcatatca acgtcgtcag tggcaatttt attattgccc agccgctggg
    15541 cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg atgaagacgc
    15601 gatccatcgt caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt
    15661 cactggcgag agctttaacc tgacctcgga agagattgcc actcaactgg ccatcaaact
    15721 gaaagctgaa aagatgattg gtttttgctc ttcccagggc gtcactaatg acgacggtga
    15781 tattgtctca gaacttttcc ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa
    15841 aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag
    15901 cggcgtgcgt cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt
    15961 gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg
    16021 cgcaacaatc aacgatatcg gcggtattct ggagttgatt cgcccgctgg agcagcaagg
    16081 tattctggtg cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat
    16141 tcagcgcgat aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat
    16201 tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agttcatcac ggggcgaggt
    16261 tctgcttgaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct
    16321 gaccacgcgc agtattcact ggttccagga acgtggattt accccggtgg atattgattt
    16381 actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc
    16441 ggatttaggg taagcgacga aaatcgccgg atgcgacatg cgtaacactc gtgcgtcgca
    16501 tccggcaatt acgtttattc ccgcgaacta aacaacgccg ccagaccact gcgccgctca
    16561 gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga cagacggcga
    16621 cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctaccggcgt gcgttggctc
    16681 ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc
    16741 cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt gccgtccggc
    16801 atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta
    16861 aacaacccaa gcgcgctgtc attacgggca atcatcaccg gtcggccttc gtaccaacga
    16921 gagtgcggat gacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc
    16981 agtccagcca cgccaaacgg cccttcccgc agggcgcaca aaagctggta ctcattgaac
    17041 gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgtccgtaa
    17101 cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa aagtcgtttt
    17161 tcgatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacgattg
    17221 atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc
    17281 aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg aacgtgagtc
    17341 cccgtcaggc ggcttagctg cctggcacgc tcggcggtaa agcccgcgtt ggcataagcg
    17401 cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg atcgccgaga
    17461 aagatcactc gcgcatgatc tggcaaggcg tcgatcagtc tcgacatcat aggcagatcg
    17521 atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc ggcatgatga
    17581 cgtaaacgct ggctacccgg ctgcgcaccc agcaatcggt gcaaagtgct ggcatcttcc
    17641 ggaatgcgtt tcttttgttc atcgctcagc ggtaactgtc gcaaagcctt gccgagagat
    17701 tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg
    17761 ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt tttaccggtg
    17821 cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgccgc cgcaactttt
    17881 tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct
    17941 tcatcaacct cgatggcatg attcacctca ttgaagaagc gcgctaccgt ccgctcgtta
    18001 caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt gggttcatct
    18061 ccccggctga ccgcttgtga agccagcaag cattcttccc aattttgtag ctcaccgatt
    18121 tcactgacac aggtcgccaa cagcggatgc gacgcctcgt tattttccag tcgtgaaagc
    18181 ggtaaacaga catgcccttc cccggcgtcg tggcttaaca gggctgccgc gagggtgacg
    18241 gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtagctgt
    18301 ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtca
    18361 taccggcaaa catctcatcc atcaggtcaa tcaaccctgc attgggtcgg gttgtgtaaa
    18421 tcccctgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata acgccgccaa
    18481 agtgacgatc atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc agcgccaggg
    18541 tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt
    18601 gggtgtaagc cgaactgtct tcacccaacc agttggattt atagtcgagc aggtaataac
    18661 gcccttcgtg gcggaacacc aggtcgataa agccttttaa catgccacgt acctgcatga
    18721 actccagcgg cgggcagcct gcggatagcg ggtcaaactg gcggattaac gtatcaagct
    18781 gactggcgat aagcggttca ctaatcggca gataaaactc catctccacc tgtttattgc
    18841 gggcggaaag ttgactcagg cttacgccgg tttcattgag aggtgcctgg aggacagccg
    18901 tgatccactc ggtcaatacc ggttcccact gcgattcaaa gccgccgagt tccagttttt
    18961 cccgcaccca gttcgggtca accggctggg taaaatccag gtcttcaaac aaactgtgca
    19021 agaatgtccc tggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa
    19081 cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac
    19141 cgtgaccacg ctgctgcaaa ccggagtagc tggtgacacg ccagttatcg ccgggcaatc
    19201 gttgtagcgt cctcgcattc agttctgcag ttaaagcatc attaacctgc cagggctgtt
    19261 tatcacccgt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg
    19321 tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt
    19381 ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga
    19441 gactgcaatg ccaaaccgaa cgtgtcagcg ccacgtaaag caaacgcaga tcttccgcca
    19501 gacgttcggc ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc agaactgcct
    19561 caaacgagtg gcgatcgtga taaaacgcct gctcctggac gcggaaattg gtgataaacg
    19621 gcagccagac caatggatat tccagccctt tcgatttgtg gatcgtgaca atctgcacca
    19681 gatgtttatc actttcgaga cgcatttgtt ggctggaggc attactgtct ggctcgagga
    19741 tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt
    19801 gtagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct gccgttgcca
    19861 gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac
    19921 gtttgcgcca gatttgccga taaccatcga actcttcaac taccgcatcc cacgcatgtt
    19981 cgtcattatt cagcgtttcg atatccagcg cgttcagccc catcattgac gttgccagcg
    20041 cactacgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacaac caaagcattt
    20101 cctgcgcttc cagagtttca aaaacactgt cgcggttcga aaggtaaacg gaagggattt
    20161 ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacac
    20221 tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc
    20281 tctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt
    20341 gataatcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg
    20401 tttcaccttt aaatacaaaa cgtaacgcct gatttttccc ggctgatttc actggaataa
    20461 acggtatttc gcgaaacatg aacgcgtcat cagtctggct gaaaagctta ttcacgctgt
    20521 tcaccattcc tggtgcggaa cgccagttgg tgtctaaagt gtagtgggcg tgaacttcgc
    20581 tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg gcctgcttcg
    20641 ggtcgccaat tagcaacaat gcggtttccg gctgatggtg ccagatacgg cgaaaaattc
    20701 ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac
    20761 ggatcgccgc tgccaacacc tcaccgcttt cgctacgcag cgcggaatcg agccgactta
    20821 acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact gtttcgcgga
    20881 tctcagccaa tgcgcgggtg atcaccagat cgcggatcga caatggttct gcaagcagtt
    20941 gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggccttc gtgcgatctt
    21001 ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg tttgtctctt
    21061 cttctgccca ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc
    21121 gtcgctcaat accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact
    21181 gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg acgggaagcc agcgtttcat
    21241 catcgggcgg cggtgctttg ataaccggcg cttcgccttg cagataacga ttaatatcgc
    21301 gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt tcacgcggca
    21361 gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat
    21421 cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc
    21481 gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca
    21541 acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggcgt tcgtacagtg
    21601 gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac
    21661 gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg
    21721 tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc aaatagagcg
    21781 ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcaccctgta
    21841 agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg
    21901 ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc agggtgtaca
    21961 tattcggctt tcccgttctg gctgccggaa atctgcgaca gaatagccat gccttgcggc
    22021 tcgaccaccg cctgatggaa gaaatcagca agtttttgcg gcgtcagcag ttttatctgg
    22081 gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac
    22141 ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggtaat taccgcctgc
    22201 tggatttgcg caaactcatc tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa
    22261 aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg caaaaggaag
    22321 cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata gcccaattgt
    22381 tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa cagagagcta
    22441 taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc cagtgcggag
    22501 tcggtgctgt taccggcttt ttcaaagatg acggattgtt ttttatcgac cactacatct
    22561 ttgtttcgac accactctga accatcagcg cccaactgtt tttgcacatc gcgtgccagc
    22621 gttgttgcct gggcctcggt catgttgccg ataaccataa actctggtcg agcccctgat
    22681 tttaaggcgt cgcgataggc cagcacctct ttcaacgtaa tggagggcaa aattttacgc
    22741 cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat aatcgcctgc
    22801 tcaaacgctt tacccttttc tgcggaatcc atcatctggt tataccagga cttcgcctgc
    22861 tcaagctgat cttccgtggc ggtatagcta aagtagcctt ccagcaatgc ctggaacagc
    22921 tgcggcagac gctgggtgta accattagca ttaaccataa ggccgttgtt agcgttggtg
    22981 gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg
    23041 agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc tttcggatta
    23101 cgcaaaatca ggctgacatc agctttgggc tcgctggcaa aataacggct tggcgcatac
    23161 accacgcgca gattcgactc atcaacaatc agctctggat ggtcgtattt cttctctgac
    23221 ttaatcagcg agaaatcatc aggaatataa gggttaagct ctggcaaaga gagcgcaatg
    23281 tcggcggctt ttttctgcca gtcggcgaaa gtttgtgcgc tgattttatc gacctgatac
    23341 ggcgcatcga caaagtaagc cgttttgttg tgcggctctt tcgggctgat ataccagata
    23401 cgcgcattct gcggcgtcat catcgccaga cgttccttta ctgctttagc atcgtaccga
    23461 tcggcaatat tgactgcatc cagcgtatgc tcaacaggaa cgcgaatcat ggtatctgcc
    23521 agccattcga cgtaatccat atcacgggtg atcgacggat aacggaagtc gatatccagc
    23581 acattcgcca gttcatcgaa gtattgttta tcaatgcctt tttcacgtaa cagattgaga
    23641 tagctaaaaa ttgccgccac aacctgatcg cgattagcca ggcctttatc ggttaaagac
    23701 gcagagatcg ctaatacgcc gctgttgccg ttgacgatag gatcggagtt ggcgctaatg
    23761 ccctcaacta atccctgctt ttgcagccag tcagaaagtg tacctgggct gcgattgcca
    23821 atcagatagg taatcaattc atcggtttta ctacggaact tcgctgagtt gttatcgatg
    23881 cgaaactcaa cgcgcaacac tttacgcggc agcgcaggga cgtaatgaat gataatgccc
    23941 ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc tttgttcggc
    24001 acgcgaccaa aggtgtccgc cgccattttt gccaactccg gcagcggttt attgctgtaa
    24061 ataaccgcct tcatcagatt ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc
    24121 tgcaccggat taccaggttt gtcgcttaaa gtttcgaggt taccaccaga aaactttgaa
    24181 ccggggtgtg ccgggttaat ggtttctgcg ctgacctgcg ccatgcgcat cccgtcacgc
    24241 gtacgcgcca tggttaattc ggcattcact gcattacgtt cacgttcggc gtatttcttg
    24301 tcgagcaagg gttctgcaat agcgtccgcc aggcggtcta ccgcaccggg caaggcgtcg
    24361 ttctcaactt ccagatagaa agccgtgcga tacggtgccg tgctggcatt gtgactgccg
    24421 ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt cgaccccatc
    24481 agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc ttccagcgac
    24541 ccaacgggca ccaccagcgc cgagagcgat ttaaccgcct gcggatcaga aaccagcaag
    24601 accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt atcactttta
    24661 cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaaggg tgcccaaagg
    24721 gcaacaaaca acaataatgc tttgaaccag gtgctgcggg gcattcacgg acctcataat
    24781 caactttatt ttctggccag attcaacagg ttaacgcaaa tcatctgcca gaatttaatc
    24841 ttgtgctgca cgagtcagcc tatgtctata tatccatcag tccgtgactg gtgcgcatca
    24901 taatgtaagc gaatgaattg cgcaattttt atacagcact catgactgat taaagcgaaa
    24961 cagcggtaac aggaaacgtt gcgactgttc aacgatggcc tccattgtct ctggtgttaa
    25021 ttgccgccag agccgttgat accagatatc atcaccttcg ccacgcacca tcatgttgcc
    25081 ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt catccagcat
    25141 ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt caggtaacac
    25201 cagcaatggc gcggacattc cttcacgata cccctcaatc agttgtgaga ggtaatgcaa
    25261 agcctgttcg gctgcaagcg gcggaaaacg ccactcaccg tctttgcgta gaaaaaggcg
    25321 actttcgcca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa gttgcattcc
    25381 ctgcgccaca cttaataaag agggacgcca gcgcaacagg ccatccggct gcacctgcgg
    25441 caaccagcca gttatctgca caccgttgca ggcgagatca atttccatac tctgccccgg
    25501 ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctggcact gtgtttccca
    25561 gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc ggcggaacaa
    25621 gcgttcggca tcatcctgct caaccagtgc attcaataac tgctgattga tttgataacg
    25681 gctaagtcct tccagaataa atggctcggt gtctgggatt tcgctgtctt cagtacggaa
    25741 gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag
    25801 cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa attcagaatg
    25861 tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc
    25921 tggctggtag ttttgtggat caaacggcat ccgggtatgg aggcaagtaa gatgcgcttt
    25981 tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat aatgactttg
    26041 cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac tgttatcctg
    26101 aatggaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta acgcttccag
    26161 gaacaggtag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt tctggctcat
    26221 caggtcaaag cccaatggcg caagctgacg tggataaacg ccgtcgttca ttcccagcag
    26281 gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc
    26341 ggcgagaaaa cgctggctga tacgttcttg atccagacgc tgcgccagtt catcacgcaa
    26401 tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga taatcgcctg
    26461 ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc
    26521 gttgagcata tcgcgacaaa ccggcaacca ctcttctagc ggacgctcct gtgccagccc
    26581 gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca gttctgcaat
    26641 taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg cgctctccat
    26701 cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg
    26761 gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac
    26821 ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg
    26881 cacatccagc agcgccagca catcctctga cacaaagcga ctgtcaggca gtgataacag
    26941 gctgataaac gcttccagta ccggatgtga ctgccgcgca cgacggtcgg aaatggcgta
    27001 aggcaggtaa cgatccgcag gtgcgctacc aaacactgcc tgaataaacg gactgtagct
    27061 gtcgatatca gccaccatca cgatgatgtc gcgcggagta agtgtcgcgt cttcctccag
    27121 catcgccagc aagcgatcgt gtaaaacttc aacttcacgc tgcgggctat ggcaaacgtg
    27181 gaaggtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac gggaaaactc
    27241 ttcgatgttc acacccgcaa cggcgcggtt ttccagttcc agaatgtcag actgaatgtt
    27301 atgcagcagg ttatctggcg tgacatcgac aaaggcgtcc agctcctggc tgctctccag
    27361 gtcagaaagg agataaatgt agtcgcgccc aagcttaccc catgaagcca gcagcgggtt
    27421 gccgacatcc tgttcaccat cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa
    27481 tagcggcaat tcgcgatctt caaaactgtg tcgacgctga cgggtcagca gtttcgccag
    27541 ataagcagga tctttaatat cgccccagta ataacggcag gggttggtaa acaggagatg
    27601 gatttcaata tgtttaccca gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga
    27661 aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg cggactccag
    27721 cgtttcgata aagcgctgat agagattggc gcggtgccaa cgcggttgtc cgagttcatg
    27781 ggtatattcc accagcgcct tccacaacgg cgcttgccag gcctgtgcct ctcccagccc
    27841 ttcaaccaaa tgtcctgttt cccagtgtgc cagccagtcc ggacgataga ccagatactg
    27901 gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc
    27961 agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt
    28021 catcagtttc cagctcatgc tctgtttgtt aaaggcactc tctttgggga tctccggtaa
    28081 cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa tgtttgccgc
    28141 aataccaaac ttttgcgaca gggtcatttg cagccactgc gccataccgg tactttgcac
    28201 cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa taaactccat
    28261 caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag cggctcctga
    28321 ctactgacga ttcgggcaat gcaggcgggt catctcgcct tctctgcccc cgggtgaaac
    28381 tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca
    28441 gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc gccagagctg
    28501 ttggtactgg tttctgctgg cgagactgtt cattaatgtt cgctggatac ccgataacgc
    28561 agtgacaatc atcaccatca gcaccatcgc aaccattacc tccggcaggc taaagccttg
    28621 ttgattcttc agggaagctg acataatgcc ccctctttca gcggacaaaa atcgctccag
    28681 ccgcgtggcg aaaaaacaat ttttccatca atgacttcgc ctgttcgcca caacgaaacg
    28741 ccttcataac cggcaatcaa taaggcttca ttatctgcca gtaaacgcaa acaaacctgg
    28801 gcatcggttc cagcgtactg cgagcactga actgctggct gcgtctgcca ggagtgcatt
    28861 tttccccacg ccagcgccga ctgaacgatg gcctggcggc gcaatgactg actttccatg
    28921 ctcacgcgag aggcaaaact gcgatcctgc tgactcattc cttgcaatag caagctaccc
    28981 aaaaccagca gcatcaggac cagagccagt gacgaaacac ctttttcgcg gttcacaggt
    29041 tgaatcctgt cacgctatag ctggcatcca ccacggtttg cggttcagac ttactggcag
    29101 cacgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt acgacctgaa
    29161 aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta ccttcacagg
    29221 atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca atctggtcgg
    29281 actcttttac cggttcgcga tcccagatac cgttactgtt cgcatcccac tgcacaatga
    29341 tacagtcacc ctgtccgaca atttccagcc cttcgccggt acagctgcca tgacaataac
    29401 ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca tcttccagcg
    29461 ccagcttacg ggtgcccgtt aaactttcac gctgtaacgc aggcagaaag cgtgccgctc
    29521 ccagcaacaa tacgctactg atcgccatag caatcaacac ttccagcaga gaaaaacctt
    29581 gctcttttac aggcatcctt ctgtttctcc ttgctgacaa agccggagtc ttccccacgg
    29641 cgaaaccacc agccaccact cgcccgttga gtttttgaag cgaatatgcc cggcccatgc
    29701 ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt cgacttcggg
    29761 ccagcgtggc acaaagacca atggtgaact gccatgacag gtattggccc cagcagcgga
    29821 actcacaagg caccataacg tcccctccct gataacgctg atactgtggt cgcggttatg
    29881 ccagttggca tcttcacgta aatagagcaa atagtcccgc gcctggctgg cggtttgcca
    29941 tagccgttgc gactgctgcc agtattgcca gccatagagt ccacttgcgc ttagcatgac
    30001 caaaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc
    30061 ggcagtatgg agcgaggaga aaaaaagacg agggccagtt tctatttctt cggcgcatct
    30121 tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat
    30181 ttaacggagg gtaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta
    30241 attagatagc caccggcgct ttaatgcccg gatgtggatc gtagccttca atctcaaagt
    30301 cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg
    30361 gacgcggttc gcggcttaat tgcagatgag tttgatccat atgattgctg tacaggtgcg
    30421 tgtcgccccc cgtccagaca aaatcgccca cttccagatc acactgctgc gccatcatat
    30481 gcaccagtaa cgcgtagctg gcaatgttaa acggcaggcc gaggaaaacg tcacaggagc
    30541 gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag aacgcatggc
    30601 acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc
    30661 gcgaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca atatgacgac
    30721 catccggcgt tggccaggcg cgccactgtt taccatacac tggcccgagg tcgccgtttt
    30781 catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtgt
    30841 cgccctgcag aaaccacagc agttcatgga tgatggaacg caggtggcaa cgtttagttg
    30901 tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg
    30961 ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt
    31021 ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc
    31081 agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgt cccatgctga
    31141 tgtactgcac ccaggcacca gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga
    31201 tgcgaaacgc gccgtaacca atcaggaaca aacctgagac agctcccatt gggcgcggtt
    31261 tacgaatata caggttgagg ataataaaca gcactacacc ttccagcaac agctcgtaaa
    31321 gctgtgatgg gtggcgcggc agcacaccgt aagtatcgaa aatggattgc cactgcgggt
    31381 tggtttgcag cagcaaaata tcttctgtac gggagccagg gaacagcatg gcaaacggga
    31441 agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac
    31501 caagaccaaa cggaatgagt ggtgcgataa aatcagagac ctggaagaag gaacgtttag
    31561 tacggcgggc gaagataatc atcaccacga taacgccaat cagtccgccg tggaaagaca
    31621 tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt
    31681 agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta
    31741 agttttcaac ttcatttttg gtccagccgc tgcccggacg attcgcccgt cgtgttgcca
    31801 gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga agcgccacgg
    31861 gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct
    31921 gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag
    31981 gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata
    32041 ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc
    32101 cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca
    32161 gactacgctg cgcaagattt tctgcttcgg cataatcaat gcgccgcagc aggtatttta
    32221 cccgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg
    32281 ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt ccatgtattt
    32341 ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcataaa
    32401 tgttcgccac ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg
    32461 tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat accattgacg
    32521 gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga
    32581 cttcacgtcc ggcacgttca atcaggcggc gtgcttcatc aacttcatcg aggcttgtga
    32641 ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca
    32701 cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc
    32761 atggattctc ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg
    32821 tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct
    32881 gttcttcttc cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac
    32941 cgatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa ccagcattga
    33001 gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg
    33061 ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg
    33121 gctccggatc gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca
    33181 gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga
    33241 tcgcagcatg ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg
    33301 gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc
    33361 aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac
    33421 gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa
    33481 atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa
    33541 acaattcgcg acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg aaaatagccg
    33601 ccgtttcttt ttgtgcaccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg
    33661 cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct
    33721 gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac
    33781 caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac aaggcagtca
    33841 actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt
    33901 cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc
    33961 gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga
    34021 tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc
    34081 caaccaggcc gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt
    34141 tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg
    34201 aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat
    34261 tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg
    34321 gcgtgactta acctctttta cgtcgataag cagatgcgtt ttgtggtttt ggcgtatttt
    34381 cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt
    34441 taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact
    34501 ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc tgcaagagaa
    34561 accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg
    34621 gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta
    34681 atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga
    34741 ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc
    34801 cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg
    34861 gactacctca atataaagct ttatatatga atgattgttt catactccag gaagacggta
    34921 aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca
    34981 gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag
    35041 ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat
    35101 agacatttta aaatattcat tatcagtcaa tgcattacat gtaattttag taatgaaaaa
    35161 gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg
    35221 ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt
    35281 tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca gttatcccag
    35341 ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac
    35401 ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa aagcaatata aatcagagaa
    35461 ttgaacaacg catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt
    35521 tttttaatca aggtatcatg acatgtccca acctcgccca ctgctctctc ctcccgaaac
    35581 tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc
    35641 ggcacttgtc gggctggtta cgccagagaa tttaaaacgc gataaaggct ggattggcgt
    35701 gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc
    35761 tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac
    35821 attcgtttgt gttgccccgt taacgggcaa tagcggggtg acctgggaaa ccagccacgt
    35881 gcgccacaaa ctcaaacgcg tactgtggat accggttgaa ggcgagcgca gcatcccgct
    35941 ggcgcagcgt cgcgttggat caccgttgct gtggagcccg aatgaagagg aagaccggca
    36001 gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat
    36061 taccgctcgt cacggggagt atttacagat acgaccgaaa gcagcgaatg cgaaagcgct
    36121 taccgaagcc attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt
    36181 gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct
    36241 ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag
    36301 atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac gctgacgctg
    36361 ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctt
    36421 cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt
    36481 ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga caaatccgct ttttacaata
    36541 ttcagtcagg aaatttctgc ccgtgacttg attctgcttc tgggtggctt gttccttatc
    36601 tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca
    36661 cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc
    36721 ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggcagcc
    36781 gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa
    36841 cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtggg ctttaccctg
    36901 attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc
    36961 tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaacccgct ctgatactcc
    37021 gtacgctctc ctgcgggagg gcgtactcac tcctgttacc ctcacagttt aagattttcc
    37081 tgctttcaag atatataacg tcagtttata aacagactat tatcataggt gggccaaatg
    37141 aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg
    37201 tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg
    37261 atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta
    37321 acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt
    37381 cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc tgccattttt
    37441 atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc gtatacccca
    37501 cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg aacagaacag
    37561 cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta accttatcga
    37621 cgttgccgaa atgtacccag tacctccgcg ccccgaaacg caagggttaa ccgaaaccta
    37681 cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg cctccaaagt
    37741 gagcggaccg tcgcgcaata atgacaaggg catccgcccg gatcaggcgc tggatcggaa
    37801 gaatatccgc gaagcgttgc atgacagcct caagcgtctg cagactgatt acctcgatct
    37861 ttatcaggtg cactggccgc agcgcccaac caactgcttc ggcaaactcg gttatagctg
    37921 gacagattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg cagagtacca
    37981 acgagcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg
    38041 ctacctgcat ctggcggaca aacacgatct gccgcgcatt gtcaccattc agaaccctta
    38101 cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt atgaagggat
    38161 cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat atctcaacgg
    38221 tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct atagcggtga
    38281 gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg gactggatcc
    38341 tgctcagatg gcgctcgctt ttgtacgtcg tcaaccgttt gttgccagca ctctgctggg
    38401 cgcaaccacg atggagcagc tgaaaactaa cgtcgaaagt ttgcatctgg agttgagcga
    38461 agacgtatta gctgaaattg aagcggtgca tcaggtttat acgtatcctg caccataaga
    38521 ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc gctgccagat ccacagcgcc
    38581 gttattgcca gcgcaaacag cgcaccgaag ccaatgccaa tgggcacgac cgggatgcct
    38641 accattaccg ccagcgagta aatgcccagc atcaacaaca tggcgctgtt ttcgccaagg
    38701 ttttgtactg caatcgcatt ccccgccccg acgctttttt taccccgctc ctgtagcaac
    38761 gcattgagcg gaacgacaaa aaagcccccc atcacgccaa tcagcatcaa caaggcatag
    38821 gctggcagca gctcgtgttg cagggaaaaa atcagtacca ccacgccaat caaaatcccg
    38881 gctggcatac agcgtgacac ggtttccagc gtaactaact tcgctgccgc acctgcgcca
    38941 accacgatac caatcgctac catcgcgttg agataggtgg gcgtagcgtt atcggtaatg
    39001 cccagcgcca ccggtaccca cagcaccaac aggaaacgca gcgtgacacc cgctccccag
    39061 aataaactgg tgcccaccag cgaaaaacgc gtttcaccat tgcgccatag cgaggtgcag
    39121 gcattcagga aactgcgggt catgttgatg agattccagg actgccccgg acgcgccgcc
    39181 gccagtttgg gaatgtagat attggcaacg accgcaccac cgtaggccag tgcgcatgcg
    39241 gccagggcga cgaggacatg ccagtcagcc agcacaccac cggctacgga accgagcaaa
    39301 atcgccgcta tggtagaagc ttccattaaa ccgttagctt tcactaactt actacccgtg
    39361 gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg ctgcagcacc aacacccacc
    39421 agcgtatagc cgagaaacgg attgatacca aagcagatac tggctgcgcc cagcagcttc
    39481 aggccgttgg caaacatcat cacccggcct ttggcgaagc tatccgccac ctgcccgaca
    39541 aacggcgcaa aaagaatgta agcacctaca aacaccattt gcaggatggg ctggctccac
    39601 tccggataaa actgcgcttt cagtaacgcc agagtggcaa acagtagggc attatcgcca
    39661 aacgcagaga gaaactgcgc cacgataacc gctttcatcc ccttcgacca caacgaagtg
    39721 ttagtgtgca ctgactcact catcgtgttg ttccgcttcg tctacccagc ttttcaacgt
    39781 gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt ttcagatagc gaatatcgcg
    39841 cggtacagca agctccggca cgccgtgctc gcgggcatac tgttgcaact tatcgcgcgt
    39901 cagttcgtta tctgtggtga aaagcaccag tgcctcgcct ttgctggcat cgctcttaat
    39961 cgcagtggca tggactttat ctggcgaaac accaagtgcc agttgttcca ccatttccag
    40021 cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg cggccctgaa tctgcacaaa
    40081 gccctgctcg tcaaaacgca caatatcgcc agtgtcatac cagccgcgct ccatttcgcc
    40141 gcgaacattc tcggcggtgg gcacttccag tacacctggc ttctccaccc gcagatagcc
    40201 gttcattatg ttcggccctt tcagttgcag gcgtccgccc ccttcgatac caggaaccga
    40261 caacaggcgc gcatccattc ctggtagaat acgccctacc gtaccgggtt tcgccgccat
    40321 cggtacgttg atagaaacga caggcgcgca ctcggttaca ccatagcctt caaggatgcg
    40381 caggccaaat ttatcctgcc aaagctgttt ggtactttct tgtagttttt ctgcgcctgc
    40441 caccacatag cgtagacgat agaagtcata cgggttggcg aaacgcgcgt agtgaccgag
    40501 gaaagtcgag gtaccgaaca acacggtgca actgcggtca tacaccaact ccggcacaat
    40561 gcggtaatgc agcgggcttg gataaaggaa cacttctgca cctgtaagca gtggcgtaaa
    40621 caggcctacc gtcagcccaa aggagtgaaa cagcggtaat gccgacataa agcgatcgtt
    40681 ggtggtgaag tcggcaatcg ttttaatctg ctcgacgttc gccagaatgc ttttatggct
    40741 atggacgacg ccttttggat ggccttcaga accggaggta aagaggatca gcgcctcttc
    40801 ttccggctgc tgttttacct gcgccaggcg cggcatcagc aaatgagcga agatccatac
    40861 tttgtcggca gtggtgacat ctgcttttaa atcttccaga tagacccagc gcacctgagt
    40921 aagttgctcc ggcagatgcc agagtttgcc tttatcgaga aactggcggg aagtgaagat
    40981 ggttttgatt tcagccgccg taatagcact ggtcagccct tttaccccag cagtgtagtt
    41041 catcattgcg ggaatgcggc gacgcgcgat ggccccaaaa atcactgccg cactgatgcc
    41101 tgcattgggc agcattaagc cgatgcgttc gccttcaaca ctgtattttt caaggatgcg
    41161 tccaacaaac agcgttttcg taagcaattt gcgataggag tctggggtaa agttgacgtc
    41221 ttcgacacat ttcttcccgg ctccgaagcg gtacattgca ctcagtaaag attcgtacag
    41281 agtttcacgc gggcgcaccg ccatacgcgc ttccatcatt atttgatgca gcatttcgcc
    41341 agcgattttg cgacggtcac gggcacgcgg cgcatccggc atcgccacct gcgttggtgg
    41401 caaaatatgc agagtaattt gcgggaacaa gcgacgttta accagacctt tcaggcggct
    41461 gaagtgcgta agttccgccc cttcaatacg cacaggaata accgttgcac cagacttcgc
    41521 cgcgacaaaa cccgcgccat cgtagatttt catcagcgag cctgtagtgg tgatgcgtcc
    41581 ttcagggaaa atcaccactg gtcggccctg ttccaccaga cgtaccagat gtttaatagc
    41641 cataggttgc gtcgggtcga gaggaacaaa gtcgataaat gatttcagcc aacgcatata
    41701 ccactgttgg cttattgagg tgtaaacggc aaacactgga cgcacaggta aaaacagtcc
    41761 aagcaaaatg ccatcaataa aagagacgtg attaggcgta attagaacgc gctcgccctt
    41821 cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa acacggcaca aatttcgaaa
    41881 aaagctaaaa agcattccaa ctccctttgc tctgattcag taaaagcgaa tggagggaga
    41941 ttacacgaga taaagaacgc gagcgacagt aaattaggtg cgaaaaaaaa cctgcgcatc
    42001 cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac cgaataatct caccaatcaa
    42061 tacctctggg atcttgattg tggtctgcac gacgactctt cgccagcgag aaaacgcaaa
    42121 ggaatgaagg gaaatgcaac gaggtgtgta aattgtcggt tactgttaca gattgatgac
    42181 cggcaaaaaa aaacctgcgc atctgcgcag gctggtgtaa ttcatgtgct caacccgaag
    42241 ttgacttcac ctatcaatac ctctgggatc accactttag caacctgaag ccaaacgcca
    42301 ccagcggtca atcgcaacag cgtttcgcaa agtgtaacca aaggtttgca atctcttttt
    42361 ttgtcttact tatttgcgtt tggctcacat tcccacgatg aaaacacgcc accccttgaa
    42421 ccaacgggcg ttttccgtaa cactgaaaga atgtaagcgt ttacccacta aggtattttc
    42481 atggcgacca taaaggatgt agcccgactg gcaggcgttt cagtcgccac cgtttcccgc
    42541 gtcattaata attcacctaa agccagtgaa gcttcccggc tggctgtgca tagtgcaatg
    42601 gagtctctta gctatcaccc gaacgccaac gcccgtgcgc tggcgcagca gaccactgaa
    42661 acggtcggtc tgatcgttgg tgatgtttcc gatccgtttt tcggtgcaat ggtgaaagcg
    42721 gtcgaacagg tggcttatca caccggtaat tttttattga ttggcaacgg ttaccacaac
    42781 gaacaaaaag agcgtcaggc cattgagcaa ctgatccgcc atcgctgtgc tgcgttggtc
    42841 gtccatgcca aaatgatccc ggatgctgat ttagcctcat taatgaaaca aatgcccggt
    42901 atggtgctga tcaaccgtat cctgcctggc tttgaaaacc gttgtattgc tctggacgat
    42961 cgttacggtg cctggctggc aacgcgtcat ttaattcagc aaggtcatac ccgcattggt
    43021 tatctgtgct ctaaccactc tatttctgac gccgaagatc gtctgcaagg gtattacgat
    43081 gcccttgctg aaagtggtat tgcggccaat gaccggctgg tgacatttgg cgaaccagac
    43141 gaaagcggcg gcgaacaggc aatgaccgag cttttgggac gaggaagaaa tttcactgcg
    43201 gtagcctgtt ataacgattc aatggcggcg ggtgcgatgg gcgttctcaa tgataatggt
    43261 attgatgtac cgggtgagat ttcgttaatt ggctttgatg atgtgctggt gtcacgctat
    43321 gtgcgtccgc gcctgaccac cgtgcgttac ccaatcgtga cgatggcgac ccaggctgcc
    43381 gaactggctt tggcgctggc ggataatcgc cctctcccgg aaatcactaa tgtctttagt
    43441 ccgacgctgg tacgtcgtca ttcagtgtca actccgtcgc tggaggcaag tcatcatgca
    43501 accagcgact aaccgcagtt aaagcaattc cagcgccagt aattcttcga tggtctggcg
    43561 acggcgaatc aaccgcgcct gaccattatc aaacagaact tctggtaaca gcggacggct
    43621 attgtagttg gatgacattg atgcgccata tgcccctgta tcatgcagta ccagataatc
    43681 acctgccttc acttccggca aggcgcgggt ttcaacattt cccccttcct gctgggtaaa
    43741 gacatcgccc gattcacata acggtccggc gacgacggtt tccaccgttg gcgcgtgttc
    43801 cagagaacga ccatcagctg ccagggcact gatatggtgg taactaccgt acattgccgg
    43861 gcgcatcaga tcgttgaacc cggcatcaac cagcacaaag tggcggctcc ccatttgttt
    43921 gacgctccgc acctgagtaa ttaatacgcc agactgcgct accaggaagc gacccggttc
    43981 aatttccagt ttcacagggt ggcccaaatg gcgggcgatt tgctcacgcg cggcattcca
    44041 cagaccataa taatgttcgg tatcaaccgc ctcttcaccc tgttgataag gaacagaaag
    44101 cccaccgccc gcagaaatag cctgtaaatc ctgaccgaat tcgatgacct gacgcaccat
    44161 agcaccacac acctgttcca gatgggcata atcaacgcca gaaccaatgt gcatgtgaat
    44221 gccgaccagc tgcagatgat gacgttgtat cacgtccagt gcggcgggca gatcggtgta
    44281 ccagataccg tgcttgctgt tttcgccacc ggtattggtt ttttggctat gtccgtgacc
    44341 aaaccccgga ttaacgcgca gccatacccg atgccctggc gaaacctggc ccagttggtc
    44401 gagcatatca acagaacccg cattcaccgg aatttgcaat tcactgacgc gttcaagcgt
    44461 cgcctgatcg ataacatctg ccgtaaaaac aatatcatcg gggtgcgttt gcggattgta
    44521 acccgccgcc aacgcacgct ctatttcgcc taacgagacg gaatccactt tcacgccctg
    44581 ctcacgcatt aagcgcaaaa tatgaatatt ggaacaggct ttctgtgcaa agcgcaccac
    44641 atcaaactgt ttcagcgctg caatctgccg acgaataatt tgcgcatcgt agacccacac
    44701 cgggcagcca aattcagcgg gcaaacgcag cagattttcg gcggtgagat cggtatcggt
    44761 gctgaacagt gaatgtggca tagcaaactc cagataagtg ctttttttat gattacgcca
    44821 catcataaaa agaataaaaa atatcgattt atgtcgagtc tatgcaaaaa tgatatggat
    44881 taccggattg cgagagagcg ctaatggccg ccgttaactt acgtcatatt gaaatttttc
    44941 atgcggtaat gaccgccgga agtctgactg aggcggcaca cctgctacac acctcacagc
    45001 caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat cgggctgaaa ttgtttgagc
    45061 gcgtacgtgg acgattacat cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac
    45121 gatcctggta cggactggat cgcattgtca gcgccgcaga aagtctgcgc gagtttcgcc
    45181 agggagaact gtctattgcc tgcctgccgg tcttttcgca atctttttta ccgcagctcc
    45241 tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa tatcgtgccc caggaatcac
    45301 cgctacttga agagtggctc tcggcccagc gtcatgattt aggactcact gagacgctcc
    45361 atacgcctgc gggaacagaa cgtaccgaat tactctcttt agatgaagtg tgtgtgttac
    45421 ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc agaagatttt cacggtgaga
    45481 actacatcag cctttcccgt actgacagct atcgccagtt gctggatcaa ttgtttacgg
    45541 agaatcaggt taaacgacgc atgatcgtag aaacccacag tgccgcgtca gtctgcgcaa
    45601 tggtacgggc gggggtaggt gtttcggtgg ttaacccgct caccgcactg gattatgcgg
    45661 caagcggttt agtggtgcgg cggttcagta ttgcggttcc gttcaccgtc agcctgatcc
    45721 gccccctgca ccgcccgtca tcagcgctgg ttcaggcgtt tagtgagcat ttacaagcgg
    45781 ggttaccgaa actggtcact tctcttgacg cgattttgtc gtcagctacg acagcataaa
    45841 agcgacagca tcctcggcat ggatcgccgc agtatcaaac acaggcaaaa cactgcgctc
    45901 ttctggcacc agtaaaccaa tttctgtgca gccaaaaatg acgccctgtg cgccctgttc
    45961 tgcaaggcga gcaatcactt gcgcataata agcgcgtgac gcttcggtaa attgccccag
    46021 acacagttct tcaaaaataa tctgattaat cttcgcccgt tcatccgctt caggaataag
    46081 acagttgatg gaaaattgtt ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta
    46141 acgcgtaccc agcagcgcca cacgagtcat tcctgccccg gtaattgcac gtccagtggc
    46201 gtccgctatg tgtaagaaag gcagagagca acgagactca atgacatccg ccactttatg
    46261 catcgtattg gtacataaca caatgccttc tgcgcccgcc cgctgtaagc caaacgccgc
    46321 ctcagccaga atatccccgg ttttgtccca ctcaccgcga cgctggcact cttctatttc
    46381 atgaaaatcg acgctatgta gcagcacttg cgcagagtga agcccaccaa gccgctgttt
    46441 aatgccttca tttatcaaac gatagtaagg aatagtggat tcccaactca ttcctcccag
    46501 caaaccaatt gttttcattc ctctctcctg atgtgtgtta ccgcaacaag gctgaataag
    46561 gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa aaagggccgg
    46621 atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt ctcgcctgcc
    46681 atcagtttgc gttcgatatg ttccagggtg acatttttgg tttccggaat gagccagaaa
    46741 gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca
    46801 atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac ccagttcgtg
    46861 gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc agagcacagg
    46921 atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat cgtcatgcca
    46981 acagagagcc atgacaggcc actggaagcc gtgccgttat caaactgcat cagacaatag
    47041 cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc cggtttacgc
    47101 cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt cagccctacg
    47161 accagagtcg caatcatctg ttgttctgtc gtcgtaaagc ccgccatttt gaagatacgt
    47221 ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg caacaacata
    47281 ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca accgccctgt
    47341 tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc cgacgtatcg
    47401 cgcagcatac gcaatacttc ttccgcctca atatgacgcc ccttttccgc cagccagcgc
    47461 gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa agcaagaacc
    47521 cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa aaacgccagc
    47581 acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg aacgttttca
    47641 cttgccattt cagaaagata cagaggagcg gtgtaagagg cgatcccgac agcaatgccc
    47701 agcaccacac gagcggcgat taacatctct acgctggtcg caaaagcgga ccctatcgag
    47761 ccgagcacaa acaggatggc ccccgccatc aggctgtatt tacgccccag gcggaacgac
    47821 agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact gaccacccat
    47881 tcctgcaaac ggctggtcag cacaaagtga tcggtaatga acggcaacgc tccggcgatt
    47941 acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga aacaaacata
    48001 ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt attgatagta
    48061 accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga acaattcaca
    48121 caggtgtcag gtcggaaata gcataaatat ggattaaatt gctgcgacat gtcgttatgt
    48181 gatggatatt ccaattttca aatt
//
LOCUS       DAZFZC010000030        46009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFZC010000030 DAZFZC010000000
VERSION     DAZFZC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.030"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(48..881)
                     /gene="fdhD"
                     /locus_tag="ACHKXP_001928"
     CDS             complement(48..881)
                     /gene="fdhD"
                     /locus_tag="ACHKXP_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO7646948.1"
                     /translation="MKKTQRKEIENVTNITGARQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1075..4125
                     /gene="fdnG"
                     /locus_tag="ACHKXP_001929"
     CDS             1075..4125
                     /gene="fdnG"
                     /locus_tag="ACHKXP_001929"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1660..1662,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7646949.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKLYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4138..5040
                     /gene="fdoH"
                     /locus_tag="ACHKXP_001930"
     CDS             4138..5040
                     /gene="fdoH"
                     /locus_tag="ACHKXP_001930"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO7646950.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5037..5672
                     /gene="fdoI"
                     /locus_tag="ACHKXP_001931"
     CDS             5037..5672
                     /gene="fdoI"
                     /locus_tag="ACHKXP_001931"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7646951.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5669..6598
                     /gene="fdhE"
                     /locus_tag="ACHKXP_001932"
     CDS             5669..6598
                     /gene="fdhE"
                     /locus_tag="ACHKXP_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO7646952.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6928..7170)
                     /gene="yiiF"
                     /locus_tag="ACHKXP_001933"
     CDS             complement(6928..7170)
                     /gene="yiiF"
                     /locus_tag="ACHKXP_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO7646953.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7388..7606)
                     /gene="yiiE"
                     /locus_tag="ACHKXP_001934"
     CDS             complement(7388..7606)
                     /gene="yiiE"
                     /locus_tag="ACHKXP_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO7646954.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(7983..8048)
                     /locus_tag="ACHKXP_001935"
                     /pseudo
     CDS             complement(7983..8048)
                     /locus_tag="ACHKXP_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8459..9400)
                     /gene="fabY"
                     /locus_tag="ACHKXP_001936"
                     /gene_synonym="yiiD"
     CDS             complement(8459..9400)
                     /gene="fabY"
                     /locus_tag="ACHKXP_001936"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO7646955.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9445..9882)
                     /gene="dtd"
                     /locus_tag="ACHKXP_001937"
     CDS             complement(9445..9882)
                     /gene="dtd"
                     /locus_tag="ACHKXP_001937"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO7646956.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(9879..10751)
                     /gene="yihY"
                     /locus_tag="ACHKXP_001938"
     CDS             complement(9879..10751)
                     /gene="yihY"
                     /locus_tag="ACHKXP_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO7646957.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(10745..11344)
                     /gene="yihX"
                     /locus_tag="ACHKXP_001939"
     CDS             complement(10745..11344)
                     /gene="yihX"
                     /locus_tag="ACHKXP_001939"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO7646958.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(11443..12228)
                     /gene="csqR"
                     /locus_tag="ACHKXP_001940"
     CDS             complement(11443..12228)
                     /gene="csqR"
                     /locus_tag="ACHKXP_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7646959.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(12262..13158)
                     /gene="yihV"
                     /locus_tag="ACHKXP_001941"
     CDS             complement(12262..13158)
                     /gene="yihV"
                     /locus_tag="ACHKXP_001941"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO7646960.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAT
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            13326..14222
                     /gene="yihU"
                     /locus_tag="ACHKXP_001942"
     CDS             13326..14222
                     /gene="yihU"
                     /locus_tag="ACHKXP_001942"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO7646961.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            14246..15124
                     /gene="yihT"
                     /locus_tag="ACHKXP_001943"
     CDS             14246..15124
                     /gene="yihT"
                     /locus_tag="ACHKXP_001943"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO7646962.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            15140..16381
                     /gene="yihS"
                     /locus_tag="ACHKXP_001944"
     CDS             15140..16381
                     /gene="yihS"
                     /locus_tag="ACHKXP_001944"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO7646963.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPC
                     IPLAPGLAPAVAAGLLDINAK"
     gene            16450..17352
                     /gene="yihR"
                     /locus_tag="ACHKXP_001945"
     CDS             16450..17352
                     /gene="yihR"
                     /locus_tag="ACHKXP_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO7646964.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKPHRLFFNIYGQRK"
     gene            17551..19587
                     /gene="yihQ"
                     /locus_tag="ACHKXP_001946"
     CDS             17551..19587
                     /gene="yihQ"
                     /locus_tag="ACHKXP_001946"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO7646965.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKNI"
     gene            19633..21018
                     /gene="yihP"
                     /locus_tag="ACHKXP_001947"
     CDS             19633..21018
                     /gene="yihP"
                     /locus_tag="ACHKXP_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7646966.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVNF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            21058..22461
                     /gene="yihO"
                     /locus_tag="ACHKXP_001948"
     CDS             21058..22461
                     /gene="yihO"
                     /locus_tag="ACHKXP_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7646967.1"
                     /translation="MSDHNPLTLKLNLWEKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYMLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            22528..23220
                     /gene="ompL"
                     /locus_tag="ACHKXP_001949"
     CDS             22528..23220
                     /gene="ompL"
                     /locus_tag="ACHKXP_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO7646968.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(23311..24576)
                     /gene="yihN"
                     /locus_tag="ACHKXP_001950"
     CDS             complement(23311..24576)
                     /gene="yihN"
                     /locus_tag="ACHKXP_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7646969.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQHTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(24678..25658)
                     /gene="yihM"
                     /locus_tag="ACHKXP_001951"
     CDS             complement(24678..25658)
                     /gene="yihM"
                     /locus_tag="ACHKXP_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO7646970.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDCGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(25666..26376)
                     /gene="yihL"
                     /locus_tag="ACHKXP_001952"
     CDS             complement(25666..26376)
                     /gene="yihL"
                     /locus_tag="ACHKXP_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7646971.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPETLAPLIAVTSSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(26593..28416)
                     /gene="typA"
                     /locus_tag="ACHKXP_001953"
     CDS             complement(26593..28416)
                     /gene="typA"
                     /locus_tag="ACHKXP_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO7646972.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            28789..30198
                     /gene="glnA"
                     /locus_tag="ACHKXP_001954"
     CDS             28789..30198
                     /gene="glnA"
                     /locus_tag="ACHKXP_001954"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO7646973.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            30484..31533
                     /gene="glnL"
                     /locus_tag="ACHKXP_001955"
     CDS             30484..31533
                     /gene="glnL"
                     /locus_tag="ACHKXP_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO7646974.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            31545..32954
                     /gene="glnG"
                     /locus_tag="ACHKXP_001956"
     CDS             31545..32954
                     /gene="glnG"
                     /locus_tag="ACHKXP_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO7646975.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAAHELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            33066..33176
                     /gene="yshB"
                     /locus_tag="ACHKXP_001957"
     CDS             33066..33176
                     /gene="yshB"
                     /locus_tag="ACHKXP_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO7646976.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(33405..34778)
                     /gene="hemN"
                     /locus_tag="ACHKXP_001958"
     CDS             complement(33405..34778)
                     /gene="hemN"
                     /locus_tag="ACHKXP_001958"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO7646977.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(34967..35473)
                     /gene="yihI"
                     /locus_tag="ACHKXP_001959"
     CDS             complement(34967..35473)
                     /gene="yihI"
                     /locus_tag="ACHKXP_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO7646978.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(35592..35758)
                     /locus_tag="ACHKXP_001960"
                     /pseudo
     CDS             complement(35592..35758)
                     /locus_tag="ACHKXP_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36055..36687
                     /gene="yihA"
                     /locus_tag="ACHKXP_001961"
     CDS             36055..36687
                     /gene="yihA"
                     /locus_tag="ACHKXP_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO7646979.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(37069..39855)
                     /gene="polA"
                     /locus_tag="ACHKXP_001962"
     CDS             complement(37069..39855)
                     /gene="polA"
                     /locus_tag="ACHKXP_001962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO7646980.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            40219..41151
                     /gene="yihG"
                     /locus_tag="ACHKXP_001963"
     CDS             40219..41151
                     /gene="yihG"
                     /locus_tag="ACHKXP_001963"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO7646981.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(41192..42622)
                     /gene="yihF"
                     /locus_tag="ACHKXP_001964"
     CDS             complement(41192..42622)
                     /gene="yihF"
                     /locus_tag="ACHKXP_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7646982.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(42777..43403)
                     /gene="dsbA"
                     /locus_tag="ACHKXP_001965"
     CDS             complement(42777..43403)
                     /gene="dsbA"
                     /locus_tag="ACHKXP_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO7646983.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(43420..44406)
                     /gene="srkA"
                     /locus_tag="ACHKXP_001966"
     CDS             complement(43420..44406)
                     /gene="srkA"
                     /locus_tag="ACHKXP_001966"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO7646984.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(44483..44752)
                     /gene="yihD"
                     /locus_tag="ACHKXP_001967"
     CDS             complement(44483..44752)
                     /gene="yihD"
                     /locus_tag="ACHKXP_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO7646985.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            44822..45406
                     /gene="mobA"
                     /locus_tag="ACHKXP_001968"
     CDS             44822..45406
                     /gene="mobA"
                     /locus_tag="ACHKXP_001968"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO7646986.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            45388..45915
                     /gene="mobB"
                     /locus_tag="ACHKXP_001969"
     CDS             45388..45915
                     /gene="mobB"
                     /locus_tag="ACHKXP_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO7646987.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT        11355 a        11733 c        11307 g        11614 t
ORIGIN      
        1 aatgaataag tcctgcaatt taatatatat cccaggactt attttaatta attgctcaaa
       61 cgctgcggat gggtataaac cgttgcccga cccggcttac aaaaacccac cagcgtcaga
      121 ttacaacgct cagccacttc tacagcaagc gtggtcgcgg cagacaccgc aaacaaaatc
      181 tctacgccgc acatcgccga cttttgcacc atttcataac tggcacgact ggaaaccagt
      241 accgcacctt gctgccagct ttccccttct tgtgaccggc ggcctaacag tttgtccagc
      301 gccacatggc gacccacgtc ttcatgcccg ccgaccagtt cgccagatgg caacatccag
      361 gcagcggcgt gagtacaacc agtcagttgc cccactggct gaaaatcgtt gagatgacgt
      421 aatgcatcat ccagtttgtt gagatcaaac gtctgggtga acggtagcgg ctgcaccggt
      481 tttccgatgt cattaagttg ctccacgccg catacgccac agcccgtacg tcccgccagc
      541 gcccggcggc gctccttcaa ccccataaag cggcggctgg aaagctcaat ttgtacttca
      601 agaccattac aggaaggaac gacatccatg ccgaagatat cgcgcggact ttctattatc
      661 ccctcggaaa gcgaaaaacc gagcgcaaag tactcaaggt ctttgggcga cgccatcatc
      721 accacatgcg aaatgccgtt gtagaccagc gcaacgggaa cttcttccgc gacctcatct
      781 aaccgtgggt gttgtaaatc atcacgccgc cataactcaa tttgacgagc acctgtgata
      841 tttgtcacat tttcgatttc ttttcgctgt gtttttttca ctttctctta accagataag
      901 aacacacaca ccaacattat ggtattctgt tacaaaccct tcctggatgg agggaaattg
      961 agccaattct ggacctttgc ggccccttcc gcaaagaaaa ataactccca ctccctgcac
     1021 acgcagcaag cgaatgtaaa tgggacgtga caatgtcgaa acaaggagca atccatgcag
     1081 gtcagcagaa ggcagttctt taagatctgc gctggcggta tggcaggcac cacggcagcg
     1141 gcactgggtt ttgcacccag cgtagcactc gcggaaaccc ggcagtataa actgctgcgc
     1201 acccgcgaaa cccgtaatac ctgcacctat tgttccgtag gctgtgggct gttgatgtac
     1261 agcctcggtg acggagcaaa aaacgccaaa gcatctatct tccatatcga aggcgatccg
     1321 gatcacccgg tcaaccgcgg tgcactttgt ccgaaaggcg ctggcctggt ggatttcatc
     1381 cactccgaaa gccgtctgaa gtttccggaa taccgtgcgc caggttctga taaatggcag
     1441 caaatcagtt gggaagaggc gtttgatcgc atcgccaaac tgatgaaaga agaccgcgat
     1501 gctaactaca ttgcgcaaaa cgccgaaggc gtgactgtta accgctggct ctccaccggg
     1561 atgctgtgtg cttccgcgtc gagtaacgaa accggctatt taacgcaaaa attctcccgc
     1621 gcgctgggta tgctcgcggt cgacaaccag gcgcgtgtct gacacggacc aacggtagca
     1681 agtcttgctc caacatttgg tcgcggtgcg atgaccaacc actgggtcga catcaagaac
     1741 gccaacctcg tcgtggtgat gggcggtaac gccgctgaag ctcacccggt cgggttccgc
     1801 tgggcgatgg aagccaaaat tcacaatggc gcgaagctga ttgtgatcga tcctcgcttt
     1861 acgcgtacgg cggcggtggc tgactactat gcccctattc gttccggtac tgacattgct
     1921 ttcctgtcag gcgtattgct gtacctgctg aacaatgaaa aattcaaccg cgaatacacc
     1981 gaagcctata ccaacgccag cctgatcgtg cgtgaggatt acggctttga agatggcctg
     2041 ttcaccggct atgacgcgga aaaacgcaag tacgataaat cctcctggac ttacgaactg
     2101 gacgaaaacg gcttcgccaa acgcgatacc acgctgcaac atccgcgctg cgtgtggaac
     2161 ttgctgaaac agcacgtttc ccgctacacg ccagatgtgg ttgaaaacat ctgtggtacg
     2221 ccaaaagacg cgttcctgaa agtctgcgaa tacatcgcag aaaccagtgc tcacgataaa
     2281 actgcctcgt tcctgtatgc cctcggctgg acgcaacact ccgttggtgc gcaaaacatt
     2341 cgtacgatgg cgatgatcca gctgctgctc ggcaatatgg ggatggcagg cggcggcgtt
     2401 aacgccctgc gcggtcactc caatattcag gggctgacgg acctggggct actgtcgcag
     2461 agtctgccag gttacatgac gctgccaagc gagaagcaga ccgatctgca aacctacctt
     2521 accgccaaca cgccaaaacc gctgctggaa ggccaggtaa actactgggg caactacccg
     2581 aaattcttcg tctctatgat gaaggccttc tttggtgata aagcgacggc ggaaaatagc
     2641 tggggctttg actggttgcc gaagtgggat aaaggctacg acgtcctgca gtacttcgag
     2701 atgatgaaag agggcaaggt caatggctat atctgccagg gctttaaccc tgttgcctca
     2761 ttcccgaaca aaaacaaagt gatcggttgt ctgtcgaaac tgaagttcct cgtcaccatc
     2821 gacccactga acactgaaac ctctaacttc tggcagaacc acggtgagct gaacgaagtt
     2881 gactcgtcga agatccagac cgaagtgttc cgtctgccat cgacctgctt cgcagaagag
     2941 aacggttcaa tcgttaactc aggtcgctgg ttgcagtggc actggaaagg tgcggacgcc
     3001 ccggggattg cgctgactga tggcgagatc ctctccggta tcttcctgcg cttgcgcaag
     3061 ttgtatgccg aacagggtgg cgcgaacccg gaccaggtgc tgaacatgac ctggaactac
     3121 gccattccgc atgagccgaa gtcggaagaa gtagcgatgg agagcaacgg taaggcgctg
     3181 gccgatatta ccgatccggc aaccggtgcg gttatcgtca agaaaggcca acaacttagc
     3241 tcgttcgccc aattgcgcga tgacggtaca acctcctgtg gctgctggat tttcgccggt
     3301 agctggacgc cggaaggcaa ccagatggcg cgtcgtgata acgccgatcc gtctggcctc
     3361 ggtaacacgc tgggctgggc atgggcatgg ccgcttaacc gccgcattct gtataaccgc
     3421 gcctccgcag atccgcaggg taacccgtgg gatccgaagc gtcagttgct gaaatgggac
     3481 ggcactaagt ggaccggctg ggatattccg gactacagcg cagcgcctcc gggcagcggc
     3541 gtcgggccgt ttatcatgca gcaggaaggc atggggcgtc tgtttgccct cgataagatg
     3601 gcggaaggtc cgttcccgga acactacgag ccgtttgaaa cgccgctggg aactaacccg
     3661 ctgcatccaa acgttatctc gaacccggct gcacgtatct ttaaagacga cgccgaagca
     3721 ttgggtaaag ccgataagtt cccgtatgtc ggaaccacct atcgtctgac cgagcacttc
     3781 cactactgga ccaaacacgc gctgttgaac gcgattttgc aaccagagca gtttgtggaa
     3841 atcggggagt cgctggcgaa taaacttggc attgcccagg gcgataccgt gaaagtctcc
     3901 tccaaccgtg gctatatcaa agccaaagcg gtggtgacca aacgtattcg tacgctgaaa
     3961 gcgaacggca aagatatcga taccatcggt attccgattc actggggtta tgaaggcgta
     4021 gctaaaaaag gctttattgc caatacgtta acgccattcg ttggtgatgc gaacacgcag
     4081 acgccggagt ttaagtcctt ccttgtgaat gtggaaaagg tgtaacggag acgacatatg
     4141 gcttatcaat ctcaagatat cattcgtcgt tccgcgacta acggtctgac ccccgcgcct
     4201 caggcgcggg acttccagga agaagtggcg aaactcatcg acgtcaccac ctgtatcggc
     4261 tgtaaagcct gtcaggtggc gtgttcagag tggaacgaca ttcgcgatac cgtcggcaat
     4321 aatattgggg tgtacgacaa ccccaatgat ttaagcgcca aatcgtggac ggtgatgcgc
     4381 ttctcggaag tggagcagaa cgacaaactg gaatggctga tccgcaaaga cggctgtatg
     4441 cactgttccg atccaggctg cctgaaagcg tgcccggcgg aaggtgcaat tattcagtat
     4501 gccaacggta tcgtcgactt ccagtccgag cagtgcattg gctgcggtta ttgcattgcg
     4561 ggctgtccgt tcgacattcc gcgcctcaac ccggaagaca accgcgtcta caaatgtacg
     4621 ctgtgcgttg accgcgtggt ggttgggcaa gaaccagcct gcgtgaagac ctgcccaacg
     4681 ggcgcgattc acttcggtac gaaagagtcg atgaaaacgc tggcgagcga gcgcgttgct
     4741 gagctgaaaa cccgcggtta cgacaatgcg ggtctgtacg atccggcagg cgtcggtggt
     4801 acacacgtca tgtacgtgct gcaccatgct gacaagccaa atctgtatca tggcttgccg
     4861 gagaacccgg aaatcagcga aaccgtgaaa ttctggaaag gcatctggaa accgctcgcg
     4921 gctgttggct ttgcggctac ctttgcggcc agtatcttcc actacgtggg tgtcggtccg
     4981 aaccgtgcgg atgaggaaga gaataatctg cacgaagaga aagacgagga gcgcaaatga
     5041 aacgacgtga caccatcgtg cgctacaccg cgccggaacg tatcaaccac tggatcaccg
     5101 ccttctgctt catcctggcg gcggtgagcg ggctgggctt tttgttcccg tccttcaact
     5161 ggttgatgca aatcatgggc acaccgcagc tggcgcgaat tctgcacccg tttgtcggcg
     5221 tggttatgtt tgcctcgttc atcatcatgt ttttccgtta ctggcatcac aacctaatca
     5281 atcgggatga tatcttttgg gcgaagaata ttcgtaagat cgtcgtcaac gaggaagtag
     5341 gtgacaccgg gcgttataac ttcggtcaga aatgcgtttt ctgggcggcg attattttcc
     5401 tggttctgct gctggtgagc ggcgtgatta tctggcgtcc ttattttgcg cctgctttct
     5461 caatcccggt gatccgattc gcgttaatgc tgcattcatt tgccgcagtg gcgttaattg
     5521 tggttatcat ggtgcatatc tacgccgccc tttgggtgaa aggcacgatt accgccatgg
     5581 tggaaggatg ggttaccagc gcctgggcga agaaacatca cccgcgctgg taccgtgagg
     5641 tccgcaagac aacggaaaag aaagctgaat gagtattcgc ataatcccgc aagatgagct
     5701 gggttcgagc gagaaacgta cggcggatat gattccgccg ttattgttcc ctcggctcaa
     5761 gaatttatac aaccgccgcg ccgagcgtct gcgcgagctg gcagaaaata atccgctggg
     5821 tgattacctg cgctttgctg cgcttatcgc ccacgcccag gaagtggtgc tgtacgacca
     5881 tccgctggag atggatctga ccgcgcgcat taaagaagcc agcgcacagg gcaagccccc
     5941 gctggatatt cacgttctgc cgcgtgataa gcactggcaa aagctgctga tggcgctgat
     6001 tgctgagctg aaacctgaaa tgagcggccc ggcgctggca gtgattgaga atctggagaa
     6061 ggcatcgact caggagctgg aagatatggc cagcgcactg tttgcctctg atttctcatc
     6121 cgtcagcagc gataaagcgc cgtttatctg ggctgcactg tcgctctact gggcgcagat
     6181 ggccaatctg atccccggca aagcccgcgc tgaatacggc gagcaacgtc aatattgccc
     6241 ggtttgtggt tctatgccgg tgtccagcat ggtgcaaatt ggcaccactc agggtctgcg
     6301 ttacctgcac tgcaacctgt gtgaaaccga atggcacgta gtgcgcgtaa aatgcagcaa
     6361 ctgcgagcag agcggcaaac tgcattactg gtcgctggat gacgaacagg ccgcgattaa
     6421 agccgaaagc tgcgatgact gcggcactta cctgaaaatt ctctatcagg aaaaagatcc
     6481 aaaaatcgaa gccgtagcag atgacctcgc ctctctggta ctggacgcac gaatggagca
     6541 agaaggctat gcccgcagtt ccatcaaccc gttcctgttt ccgggtgaag gagagtaatc
     6601 tctgacgttt tccgggtggc gcaggtcgcc cggattacag ctcatagggt gtgacgacac
     6661 catctctcgt attccgcgtt accacctcaa gctatgccgc cagtgccggt ttggcctcaa
     6721 tttacccacc accacatcaa taaggttatg ggcatgagac tgatgacaag cgtaagattg
     6781 cctgatgcgc tgcgcttatc aggcctacca ataaactgca atacattgaa tttatacgac
     6841 tttgtaggcc ggataaggcg ttcacgccgc atccggcatg aacaacgcgc acgttgtcag
     6901 caatctgatt gacatcattt tttcccatta ccattcctcg cgcagcttac gctgatattc
     6961 gacaccatct tcctcacacc cttgccagat gccaggaaca ctggttcttg ctgtttgcga
     7021 ttgatttatc aggtattgat ctaccgcttc ccttaatagg tccgcacgag gaagattacg
     7081 ctgcacctcg agatcatcaa gttgcttaat cacctcattc gataaatcga gtaaaattct
     7141 gcccatatct atacctgcca gagagttcat aaatatctcg ccaatatcat ttttgaatct
     7201 atggagagaa aagtaccctt gccgaatctt taaagaaagc tcatttacgc atcatttttt
     7261 atttttgcga ggcgttttcc agatatgatg taacagttgc agttattata agttcaagta
     7321 aaacagcctt gtattaatac cagaataagg gcatctgttt tttatattca agaatgaaaa
     7381 atttttgtca ttccttatgc tccttacact gatcatcaac accaactacc tccccctttt
     7441 gcctgcctcc ctttgcttta cgcaccggga attgctgacg ttccagatat tgctctaccg
     7501 cttcgcgtaa aattgcagat cgtgaaacct tacgccagcc ccgaagcttg ttcagccgtt
     7561 taattgcctc ttctgataaa tgaagaaaaa ccgtattcat cgccattacc aataccttac
     7621 atatattact cattaatgta tgtgcgaaaa ttacgccgga aaatcattta aaaaaagcgg
     7681 atttttgcat gttgttttgt ttttgcgagg cgttttccag aaataataag agtgttgcag
     7741 ttgtttcatt tattactcaa aaatggttta gttatcgccc aagatgaatt atgggtgcta
     7801 tgaatatatt atattcagag atatttattt gcataatctg aatggtgtag ccgaaaaacg
     7861 tcagcatcct aacagcacca ggatttaagg tgaaattaat ctttcatcac agaccggtca
     7921 gtactgccac caaccggtct ttacttgctc atcgttataa ttttgttggt aacagttcgg
     7981 gaaagttgtt tttctaaatt aacaacagaa taactttctt gtttaatgag attaatattg
     8041 gcaaccatta tagcctcctt cccacggttt aagagcaaac atacaacata attattcata
     8101 acatacagag cagtaaatga atgatactga caatgatgct agtccattat ctttgcataa
     8161 tatttgttag ctatagattt taaaaatatt tatgagaaaa gtatggcacc agagggggtt
     8221 ttgactatca aaaatcgctc aaacatgaac taagatgatg aattaagcat tatctgcgat
     8281 aattgccact cagttctaca aataatattt aaatgctcct gaacagtgat gtagcaagac
     8341 acgtatggag ccaacgaaga ataaaaattt atagagttct tatttaatat aaccgaaata
     8401 gccaaggact aatataaatg acctgaatga gagaggcagc accgcccctc tccatcagct
     8461 actcttcttc gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc agaacgatat
     8521 acgtgccttc aaacactgca cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca
     8581 cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc gcttaaggca ccgaggtcgg
     8641 ctaccgcatg aggtttaccg ctaatcggct tgctgtagcg gatatgcgca tccgcaagaa
     8701 taatcgttcc gccgaggtgg cgttcacgca gcatcagcca gataagtccc caaccggtga
     8761 gcgtcgccag tgagaataaa ctcccggcaa acagcgtatg gtgcggattc tgattgccgg
     8821 tttctggcat ggtagtgata aatttttgcc cggtatattg ctgaatgcgc acgcccattt
     8881 tttcactaag cgggatatgt tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc
     8941 gatgcaaaat atcatccaga gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg
     9001 tggtggttgg cgtggtgatt tctccctgat taacaaaccc cagcttggcg aaaaactcca
     9061 ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc ttcctgacgc gccaccgact
     9121 ccagggtcat cgccatcagc gttcctaacc ctttgtcctg cacgtccggg tgaacggcca
     9181 taaagcgaat ggacgcttca ttgtcggcat taatatacag tcggcctacc gccaccagat
     9241 taccctgctc gtcgacgacc atctggtgat gcgccatcgc atcccacgcg tcgcgttccg
     9301 aaccttttgg ttgatgcagg ggcttacgca acatttccca gcgaaactga tagtaacgct
     9361 ctaattcttc ttctgtttgt ggaacccgaa ggtgatacat agcgatactc tctcttgttt
     9421 cccgtgacaa ccctggaagc tggctcatac ctgcaaccag aatgtcacgg ggccatcatt
     9481 gaccagcgat acctgcatat ccgcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg
     9541 acggcagcgt tcgacgaaat agtcatataa cgcctctgcg cgatccggtg atgcaccttt
     9601 ggagaaactt gggcgcatcc cccgttcggt atctgcggcg agggtaaact gggaaaccac
     9661 cagcacactg ccgcccgcct gttgcacgtt gagattcatc ttgccttcgg catcgctaaa
     9721 gatgcggtag ccgagcacac gctcgcacag acggtttgct ttctgttcgt cgtcatcctt
     9781 ttcgacaccc aataacacca aaagtcccgc gccaatttcg cccgtcactt ctccctccac
     9841 ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc atggttcgtc gtcttcttct
     9901 tgttcagctg cttgttttag tttgcggtat tccccgagag tgacagtaat ttccgcgcca
     9961 agcaagacga tacaccatgt ccagtagacc caaacaaaga gaatggggat caccgccagc
    10021 acaccgtaaa tgagctgata tgacgggaac atggtgatat aaagcgcgaa acctttcttt
    10081 cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg
    10141 cggatggtag gaacaatgct gtacagcaac cagaaggaga tccacgacaa cagcaacgga
    10201 aaaatacgca gcacgttgtc gatgacagta ttgagatcgc tcgcccagcg cagggagagc
    10261 aaataggaac tgatcgccag actggcccct gccagcagcg gccccagcgt taaaatcatc
    10321 cagtacacgg cgaacgagta aatttttggt cgcgctcgtt tactgcgcca gatggtattc
    10381 aacgcgctat cgatggagta catcaacaat aacgcggtga cgatcagccc gcacgcgcca
    10441 acggcggtca tcttgttgga attggcaaca aattgttcga tataccgctg aataacatcg
    10501 ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta actgaatgct gacgtcggaa
    10561 aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa ccagcggcac taatgagagc
    10621 aacgacacat aggcaaggtt acctgccagg gttgtcatgt tgtcctcatc aatgcgttgc
    10681 cagagtagtt ttagccaggc ccatagtgga cgggtacggt gcctggcttt gtcctgaatg
    10741 gtttttagca taacaccttc gcgaaatagt ccgggatggt ggttttatct ttcaccagaa
    10801 tactggtaat gcccagctga ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga
    10861 ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag
    10921 gtttgcgcat ccccagatct tgcgacagat agatatggtc agcagcatca cgaatttccg
    10981 ggtattcttc cggccagaag gtggtatgca ggcggttggt attggaaagc accaccacgc
    11041 gatgcccctg ctcacgcagt ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa
    11101 acaccgcctg ccagccgtga gagaactgct cgtagcttag cggtagagcc atctcatgac
    11161 acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct
    11221 cccccatatg aaaactcttc ttaagcgtag ccagcggaat acgcgttaaa tcgctccagg
    11281 ctcccagcac acggttaaag tcgatatcga caatcacatt acctaaatca aagatataga
    11341 gcatttttgc ctcctttcgc gccacaagaa attaactgta gcgggaaagg atgggtttgg
    11401 ctatgtggca aggtatgaag ccaggtcaac ggtcacaaaa atctacgcgt cttcctgggg
    11461 aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc
    11521 gtcggtaaag agcgcggtga cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg
    11581 atacttagtg tgatcggcga ccagcaggat atttctcgcg tgcgccatca tcgttttcac
    11641 cacgttagct tcgttaacat caaactccat caacgcgcca tcgctctcga ttgcgcctac
    11701 gcttgttacc agataatcag cacgaaaatc ggccacaaag gacgccgctg aagggccaat
    11761 gatcccgcta ttatgagagc gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt
    11821 gtggtaaaga atatgcgcca cacgcaggct gttggtgatt atccgcaaat gattatggtt
    11881 aagtaacgcc cgggcgacat gctcaacggt cgtaccaatg gtgataaata ttgttgaacc
    11941 atcaggaata tagtctgcca ctgcttcggc aatcgctttt ttttcctcgg tttgcgaaac
    12001 ctcacgctgc tcgaacgccg tattaacgac gctggaagcc cgacccgcgc caccgtgatg
    12061 gcgcgtaatc aggccttgct cgcttaattt acgaatatcc cggcgcaccg tctgcgtgga
    12121 gacatccagc agatttgcca gctcatcaat attcatatac ccacgctcgg cgatcagcat
    12181 gaggagttgg tcgtgccgcg ggttaccggt tagttcggta aggctcatga aaattcctcg
    12241 aaaaaccatc accctggcat tttatacaaa aagtgacaaa aaagatcggg tttgatcaca
    12301 gtcagggatc ccggctcgtc caccgggacg cgtgcatttc aacgccgcta caccgctggc
    12361 gaagcggact gactccgcta aatccccacc tgttgccagc gccaccgcca atgcgccgtg
    12421 aaaaacatca cccgcacctg tggtatctac cacatcaact ttgaaggccg gttgatgttg
    12481 acgcccgcca ttttccagcc agtcacagcc tgcgctgccc tgggtcacat agacatgacc
    12541 atttgtgagc gtttgtgcct gttttagcgc actggccatc tctttcacgc ccgttaagcg
    12601 cgccagaccc ggttctgaaa aggccgcatg atcgcttaat gccaccagct cgctgatatc
    12661 ctgcggcgta atatcaccgt ccagaacggt catcacgccc gcctgccggg ccagagtgaa
    12721 ggctttttta gcgccgtcgt gccagcgtac atctgccagc acaacatccc actgagagaa
    12781 atcaatttcc tccaaccact ctgcgtcagg cagcaggtcc gggctggggt agttaatgat
    12841 tatccgctcg cctgtggcat ccaccatgat ggcggattgc gatgatttcg cctggttata
    12901 ccgtttggtg taacgggtgt taacgcccca ggattccagc tctgccagca ggctgttgcc
    12961 cgtgtcgtca tcacctacgc gaccaataaa atcaacctgc gcccccagcc ttgccgccgc
    13021 aaccgctgcc gtcgccgctg gcccgccacc aacttccgta taatttctcg ccacgtattt
    13081 accgctctcc gtcggtaacc cttccacgta atagatgcga tccatcacgg ttatacctac
    13141 acaagcaaca cgaatcatgg tcattcctta aacattttgg gatgcgttca ttttaatttc
    13201 accaattgtt taaaaagtga cggctgtcaa ttttttgact ataaattaca aatacgatca
    13261 aaaacagaca atcaaagcgt gtcataaatg acaaaaagtg acatcgctgt attcaggaga
    13321 aggttatggc agcaatcgcg tttatcggtt taggacaaat gggttcgcca atggcgagca
    13381 atttattgca gcaagggcac caacttcgcg tctttgatgt gaatgccgag gctgtgcggc
    13441 atctggtaga caaaggcgcg actcccgccg ccaacccggc gcaggccgct aaagatgccg
    13501 aatttatcat taccatgttg ccgaatggcg atctggtgcg cagcgtgttg ttcggtgaaa
    13561 acggcgtttg cgaaagctta tctaccgatg cgctggtcat tgatatgtcg accatccatc
    13621 cgctgcaaac cgataaattg attgccgata tgcaagccaa aggcttcagc atgatggatg
    13681 ttccggtagg ccgtacttct gcaaatgcca ttaccggtac tctgttactg ctggctggcg
    13741 gcaccgctga acaagttgaa cgtgccacgc cgatcctgat ggcgatgggc agtgagttga
    13801 ttaacgctgg cggtccgggc atggggatcc gcgttaagct catcaacaac tatatgagca
    13861 tcgcgctcaa tgcgctttcg gcagaagctg ccgttttgtg cgaagccctg aatcttccct
    13921 tcgatgttgc cgtcaaagtg atgagcggta ccgccgccgg taaaggccac ttcaccactt
    13981 cctggccaaa caaagtcctc agcggagatc tttctcccgc cttcatgatc gatcttgccc
    14041 ataaggatct tggcatcgcc cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg
    14101 gggccgcctc acgggaggtt tatagccagg cgcgcgcagc gggtcgcggt cgccaggact
    14161 ggtccgccat tctggaacag gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt
    14221 aacgactgga taaaggaata aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc
    14281 atcgggcggt tttgccatgc tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc
    14341 cgcggctggg gcacccgctc cggtagccga tagcgtttta actgatttca aagttaacgc
    14401 ggcgaagacc ctctcgcctt atgcctcggc gattctggta gatcaacaat tctgttaccg
    14461 ccaggtggtt gagcaaaacg cgattgccaa aagttgcgcc atgattgtcg ccgccgatga
    14521 gttcattcct ggcaacggta ttccggtaga tagcgtggtc attgaccgca aaatcaatcc
    14581 gctacagatc aaacaagacg gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga
    14641 tgaagatgcg cagcaacgcc tggatatggt gaaagagttt aacgaactgt gccactcaca
    14701 cggcctggta agcattattg agccagtcgt ccgcccacca cgtcgtggcg ataaattcga
    14761 tcgcgaacaa gcgatcatcg atgctgccaa agagctgggc gacagtggcg ctgacctcta
    14821 caaagttgaa atgccgcttt acggtaaagg tccgcaacaa gagcttctca gtgcttcaca
    14881 acgtctgaat gaccatatca atatgccgtg ggtgatcctc tcttccggtg tcgacgaaaa
    14941 actgttcccg cgtgccgtac gcgtggcaat gacagcgggt gcatcgggct tcctggctgg
    15001 tcgtgccgtc tgggcatcgg tcgtcggttt accagacaac gagctgatgc tgcgtgacgt
    15061 ttgtgcaccg aaattacaac aactgggcga tatcgtcgac gaaatgatgg ctaaacgccg
    15121 ttaagatggg gatacaaaaa tgaaatggtt taacacttta agtcacaacc gttggctgga
    15181 acaggaaacc gatcgcatct ttgattttgg taaaaattcc gtagtaccga ctggatttgg
    15241 ctggttaggc aataaagggc aaatcaaaga agagatgggc acccatctgt ggatcacagc
    15301 tcggatgttg cacgtttatt ccgttgctgc ggcgatgggt cgacctggcg cttactcgtt
    15361 ggttgatcac ggtattaaag ccatgaacgg cgcactgcgc gataaaaaat atggcggctg
    15421 gtatgcctgt gtgaatgacg agggcgtggt ggatgcctcc aaacagggct atcaacattt
    15481 ctttgctctg ctgggtgctg ccagcgccgt cactaccggt catccggaag cgcgcaagct
    15541 gctcgattac accattgaaa ttatcgagaa atacttctgg agcgaagaag agcagatgtg
    15601 cctggagtcc tgggacgaag ccttcagcaa aaccgaagag taccgcggcg gcaatgccaa
    15661 tatgcacgca gtggaagctt tcttaattgt ttatgacgta acgcatgaca aaaaatggct
    15721 ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac gtcgcaagaa ataatcatta
    15781 tcgcgttaac gaacacttcg atacccagtg gaatccgctg ccggattaca acaaagataa
    15841 cccggcgcac cgcttccgcg cgttcggagg tacaccaggc cactggatcg aatggggccg
    15901 tttaatgctg cacatccacg cggccctgga agcccgttgc gaacaaccgc cagcatggct
    15961 gctagaagat gccaaaggtc tgtttaacgc caccgtgcgt gatgcctggg cacccgatgg
    16021 tgcggacggg attgtttata ccgttgactg ggaaggaaaa ccggtggtcc gcgaacgtgt
    16081 gcgttggcct atcgtcgaag cgatgggtac ggcctacgcg ctctacaccg tcaccggcga
    16141 tcgccagtat gaaacctggt atcagacatg gtgggactac tgcattaagt acctgatgga
    16201 ctatgaaaat ggttcctggt ggcaggagct ggatgcggac aataaagtca ccacgaaagt
    16261 ctgggacggt aaacaggata tttatcacct gctgcattgc ctggtgatcc cgtgtatccc
    16321 gttagcgcca ggcctggctc cggcagttgc ggcgggtctg ctggatatta acgcgaaata
    16381 gctgactaaa aaaaaacgac atatcaggct gcacgccggg tatgtcgtta ttcaataaag
    16441 gtacctcgta tgcaaatcac caacatgcat tgcagtggac agaccgtttc acttgccgcg
    16501 ggggattatc acgcgaccat tgtgaccgtc ggtgcaggtc tggctgaatt aacctttcag
    16561 ggctgtcatt tagttattcc acataaaccc gaagagatgc cgctggcaca tctgggcaaa
    16621 gtccttattc cgtggccgaa ccggatcgcc aacggctgtt atcgctatca ggggcaggaa
    16681 tatcagttac ccattaatga acatggttca aaagccgcta ttcatgggct gctcgcctgg
    16741 cgtgactggc aaatcagcga attaaccgcc accagcgtta ccctgacagc ttttctgcca
    16801 cccagttacg gctatccgtt tatgctggct tcgcaggttg tttattcgct caatgcacgc
    16861 acgggtcttt ccgttgagat cgcaagtcag aatattggta ctgtgaccgc accctatggt
    16921 gtgggtattc atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag
    16981 cttccggcaa accaggttta tgccgtcgat gagcacgcta atccgacaac gcttcaccac
    17041 gttgatgagc tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac
    17101 cacaccttta aaaccgctaa tgatctgtgg gaaataacca ttacccatcc acaacaggcg
    17161 ctttcggtaa gcctttgttc cgatcaaccc tgggtgcaga tttatagcgg cgaaaaatta
    17221 cagcgtcagg gactggcagt tgaacctatg agttgcccac ctaacgcctt taattcaggt
    17281 ctcgacttat tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga
    17341 cagagaaaat aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta
    17401 aaggcaacat tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt
    17461 tttatatatc agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca
    17521 atctcgttct caactctatg gagattagtt atggatacgc cacgtccaca gttattagat
    17581 tttcaatttc atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta
    17641 acccatagca aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg
    17701 ttccgcggta atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc
    17761 atcgtcagcc agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc
    17821 gccacgctga atatctctgc cgacgatcag gggcgtttat tgctggaact acaaaacgac
    17881 aaccttaacc acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac
    17941 ggctgcggcg aacagttttc ctacttcgat ctgcgtggca aaccgttccc gctatggacc
    18001 agtgaacaag gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa
    18061 gaaaatgcgg gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg
    18121 cagaagtatt actgccatgt tgataacagt tgctatatga acttcgactt tagtgccccg
    18181 gaataccatg aactggcgct gtgggaagac aaagcaacgc tgcgttttga atgtgctgac
    18241 acatacattt ccctgctgga aaaattaacc gccctgctgg gacgccagcc agaactgccc
    18301 gactggattt atgacggagt aacgctcggc attcagggcg ggacggaagt gtgccagaag
    18361 aaactggaca ccatgcgtaa cgcgggcgtg aaggtcaacg gcatctgggc gcaggactgg
    18421 tccggtattc gtatgacctc ttttggcaaa cgcgtgatgt ggaactggaa gtggaacagc
    18481 gaaaactacc cgcaactgga ttcacgcatt aagcagtgga atcaggaggg cgtgcagttc
    18541 ctggcctata tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa
    18601 cacggctatc tggcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt
    18661 tacggcggcg ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc
    18721 aaaaagaaca tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg
    18781 cccaccgaca cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct
    18841 gcgctgtggg cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc
    18901 cttttcttta tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg
    18961 ggcgaccaga acgtcgactg gagtctcgac gatggcctgg cgtcggttgt cccggcggcg
    19021 ctgtcgctgg caatgaccgg acatggcctg caccacagcg acattggcgg ttacaccacc
    19081 ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc
    19141 acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc
    19201 gacgcagaaa ccatcgccca tttcgcccgt atgaccaccg tcttcaccac cctgaaacct
    19261 tacctgaaag aggccgtcgc gctgaatgcg aagtccggcc tgccggttat gcgcccgctg
    19321 ttcctgcatt atgaagacga tgcgcacact tacaccctga aatatcagta cctgttaggt
    19381 cgcgacattc tggtcgctcc ggtgcatgaa gaaggccgta gcgactggac gctctatctg
    19441 ccggaggata actgggtcca cgcctggacg ggtgaagcgt tccggggcgg ggaagttacc
    19501 gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg ccgacagcga atgggctgca
    19561 ctgttcgcct cgttaaaaaa catctaatca tgcccgcccg tggggaaact cacgggcaaa
    19621 tggagaataa cgatgagtca catcacaacg gaagatccgg caactttgcg cctgcccttt
    19681 aaagagaaac tctcttacgg tatcggcgat ctggcctcta acatcctgct ggatattggt
    19741 acgctttatc ttttgaagtt ttataccgac gttctggggc tgcctggcac ctatggcggc
    19801 attatctttt tgatctcgaa attctttacc gcctttaccg atatgggaac cggcatcatg
    19861 ttggattccc gacgcaagat cggtccaaaa ggtaaattcc gtccttttat tctgtatgcg
    19921 tcattcccgg ttaccttatt ggcaatcgct aactttatcg gcacaccgtt cgatgtcacc
    19981 ggtaaaacgg tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg
    20041 aactgctcct acggcgcaat ggttcccgcc attaccaaaa accccaacga acgcgcctcg
    20101 ctggcagcat ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc
    20161 gtgccggtta tgaatcttat cgagggtaat cagcaacttg gctatatctt cgccgccacg
    20221 ctgttttcac tgtttggcct gctgtttatg tggatctgct attcgggcgt gaaagagcgt
    20281 tatgtcgaaa cccaacctac caatccggcg caaaagccag gcctgttgca atctttccgc
    20341 gcaattgccg gtaaccgccc actgttcatt ttgtgcattg ccaacctctg taccttaggg
    20401 gcgtttaacg tcaagctcgc cattcaggtc tattacaccc agtacgtgct taacgatccc
    20461 atcctgttgt catatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg
    20521 atgcctggtg cagtcaggcg ttttggtaag aagaaggtct atattggcgg tctgctgatc
    20581 tgggtgctgg gcgatctgct caactatttc ttcggcggcg gctcggtcaa ctttgtggct
    20641 ttctcctgcc tggcgttctt tggttcggcg tttgttaaca gcctgaactg ggcgctggtt
    20701 tctgacaccg tcgagtacgg cgagtggcgc accggtgtcc gttctgaagg gacggtctac
    20761 accggcttca ccttctttcg caaagtttct caggcgctgg caggtttctt ccccggctgg
    20821 atgctgacgc aaattggcta tgtgccgaac gtcgcacagg ctgaccacac cattgaaggg
    20881 ttgcgccagc tgatcttcat ctacccaagc gcactggcgg tagtcaccat tgtagcgatg
    20941 ggctgcttct acagcctgaa cgagaagatg tatgtccgca ttgttgaaga aatagaagcc
    21001 cgtaaacgca cggcgtaata ataaacgacg ccctgcgggg cgttataagg agtgattatg
    21061 tctgaccata atccactgac attaaaactg aatctgtggg aaaaaatcgc ctatggtatg
    21121 ggcgacgttg gttcgaattt aatgcttagc atcggtactc tgtatctcct caaattttat
    21181 accgatgaat tagggatgcc agcttactat ggcgggatta tctttctggt ggcgaagttt
    21241 tttaccgcgt ttaccgatat gctcaccgga tttttactcg actcgcggaa aaatattggg
    21301 cctaaaggca aatttcgtcc ctttatttta tatgcagcgg ttccggcggc gttaattgca
    21361 acgctccagt ttatcgccac cacttttagt ttgccggtta aaacgacgat tgccacggcg
    21421 ctgtttatga tgtttgggct ttcatacagt ctgatgaact gctcgtatgg cgcgatgatc
    21481 ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt
    21541 gcaaccatag ggctattgat ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct
    21601 gactcaaccg tcggttatgc ctgtgcggca cttatgttct ccattggcgg ctttattttt
    21661 atgatgctgt gctacagagg cgtcaaagag cattatgtgg acacagcacc aaccggacat
    21721 aaagccagta ttctcaaatc tttttgcgcg atatttcgga atccgccatt gctggtttta
    21781 tgcattgcta acttgtgtac cctggcggca tttaatatca aactggcgat tcaggtctat
    21841 tacacccagt atatgctgaa tgatattaat ttattgtcgt ggatgggatt cttcagtatg
    21901 ggatgcatcc tcgtcggcgt attactggtg ccattaactg taaaatgttt tggtaaaaaa
    21961 caggtttatt tagctggcat ggtgctatgg gcggttggtg atatcctgaa ttatttctgg
    22021 ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt
    22081 gtaaacagcc tgaactgggc gctggtacca gataccgtag attacggtga atggaaaacc
    22141 ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa aatttctgcc
    22201 gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa tcggttatgt tcccaacatc
    22261 gcgcaaagtg atgcgacatt acagggtttg cgtcagctca tttttatctg gccttgtgca
    22321 ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga aaaacggttt
    22381 gcgttgatta ttgaggaaat caaccaacgc aaaaataaag aaattgagac agaagaaaaa
    22441 acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt gggtggcggc
    22501 ctccctacgt ttaaaaatgg acttattatg aaaaatatta atgcaataat tttattatct
    22561 tctttgacct ctgcctcagt attcgctggc gcatacgtcg aaaacaggga ggcatataat
    22621 ctcgcctccg atcagggcga ggttatgcta cgtgtgggtt ataacttcga tatgggcgcg
    22681 ggtattatgt taaccaatac ctacaccttt cagcgagaag atgaactaaa acatggatat
    22741 aacgaaatcg aaggttggta tccgttattt aaaccaaccg ataaattaac catccagccc
    22801 ggtggcttaa ttaatgataa gagtatcggt tcgggtggtg cagtgtatct ggacgtcaac
    22861 tataaatttg taccatggtt taatctgaca gtacgaaatc gctataacca taataactat
    22921 agttcaacag atttgagcgg ggaactggat aataatgaca cctatgaaat tggtacctac
    22981 tggaatttta aaatcaccga taagttctcc tatacatttg agccacatta ttttatacgc
    23041 gtaaatgact ttaatagcag caatggaaaa gatcatcact gggaaatcac taacaccttc
    23101 cgttatcgca ttaatgaaca ctggctgcct tatttcgaat tacgctggtt agatcgtaat
    23161 gtcgaaccgt accaccgcga gcaaaaccag attcgtatcg ggacgaagta tttcttctga
    23221 tgtgaaactg ccggatgcgg cgtaaacgcc ttatccggcc tacggttgat acggttttca
    23281 ttggccggat taagcacatc cggccattaa ttaagcttca gccgccagca tctctttctt
    23341 acgttggcta atacgcttaa ccagcattac ggaaaccacc gcaccacaga atgcgaagca
    23401 ggccataatg ccaaacacga ttttgtagcc aataatcccc ggatttaaat ccagaatgta
    23461 gccatacaga ctgaagcaga acatcgccgg ggcgtaacca ataaagctac ccaacgccat
    23521 cgccgcgcct gttttatttt cagcaatttt tgcttcgccg ataggtgcaa aaaatacggc
    23581 tcgctgtgta aagactatcg cgccaaagcc cagcgtacat gccatcccta aatagaccgg
    23641 catactttcg tgcggcagca taatcaacag tacgagcgca gcggtactga tgataaaggt
    23701 gtagcataga tatttactcg gcgatttcag gatcttatct gaaatcatgc caccaatcgg
    23761 tccaccaatc attttcagac agtattggtt aatgatgccg taagccccca ccagcgcaac
    23821 gggcaataga tagatgtttt tcaggaatgg aatgaagaat gtcaggccac agtaaaccgc
    23881 gtaaacgaag aagacgttga aagcgataag ccagatagtt ttgtctttca gcaccgaggt
    23941 catactggtg tgttgcgatg ccccatcttc ttttttcacc tcaacggacg gtgcctcttc
    24001 tttgtcattc aggacaaaga aaataataat gcctacggca atcacaataa gggaatagaa
    24061 ccagatgcct gctttaaaac ctaataagcc actgccaaac caggtaaata ctgccaacgc
    24121 agaaaatgcc accacggtat cgacaatgcc acgccctgtt tcgaagaagc caaacaaccg
    24181 accttgttgt tcgctattgc ccaatcgact taccgacttg agcaagaccg gccagttcat
    24241 catgtcgcaa gtaacgccaa atagcgccca gacaaagagg atgccccaat accccggcat
    24301 tgtcgtcaaa taaacaccca gtaatcctgt cgcaatgagt gagaatgaca tggtgtatct
    24361 gcgcggtagt ttatcggcaa aataaataga cagaaaaaag cccactgtgg taacaaatga
    24421 gtttaccgac atggcattac caatttgacc attggtcaaa tggaaatatt cctgcatcgg
    24481 gatataaaac gcatctttca gcgacggtaa tttataaatt gtaccgccac acagtgttaa
    24541 tagactaaat aacgcccatt tctttttcgt gagcataaca tcccctaagc gatttttaaa
    24601 atcactgttt taaaggcaat aagataccga ccattcatat agaatggttt tattaactgc
    24661 gtatgagtta aaacttatta gtggttcaga agatggtttg cctcttgttt gatttgttgt
    24721 agtacgttac gcacatgatt tatctgatta attgcatctt ctttttcttt acgtaaccgc
    24781 gcatccagta aatgattttc tggtaggggt gtttcactca tctggcgata agggatccac
    24841 ggattatcat cttcgtcttc aaagcggaac gccggcgcat agtaatcaac ctcttcttcc
    24901 aggccatatg ccgtcacctg cggctcatca tcaccaaggc agataaggtg cgttaataac
    24961 gctttgaacg gcatatcacc ctgacctgaa atacaggctt tatgacccag gccaccctgt
    25021 tctttaacga tcaaggcatc tttaatatgg acctgggtaa tatgcggtgc catcgttttt
    25081 aaggcgtcga tgggatgctc atttgcattg atcatgttcg caaaatcaaa cagtaaggaa
    25141 agagactcca tctcactttc tttgaccagc gacaccagct catgactttt taaatcttca
    25201 tgctgctcaa tcgtaaaagt caggccgcag tcctgatacg tttcccgtac ataggcaatg
    25261 tcgttagcga taatcgataa tacgtcgcgc aaattacctt cataacgtgg ataaaaacgt
    25321 acggacgatg ccccagtttt caacgcgatg gcgacggctt cgtcaataga tgccttatct
    25381 gaggcgctgg tttcaatatg aatatcaagg ttcagacggc gggctttatc tccaaaggca
    25441 gagagttctt tatcgtccat attaccaaga gaaaaacgct cgccatccag aacatggatt
    25501 ttgacgccac gtagattatt ttcacttgcg atatcaagta aatcagcagg caacactctt
    25561 tccagccgca tatttaaatg aaaggcataa gcatgtaaat aaagaggaag agtgtcgaca
    25621 cgttgtagaa tctttcttgc gttatttatt gtcatcataa aacacctaca gattaatgat
    25681 gaggtctacg gcctcaaatc gccaatattc aatatctatc tggacaatat tgccttgctg
    25741 gtcggcgcga tgcttttcaa ttaagatcgc tggcataccc gcagaaccgc caagatactt
    25801 gctgatatcg cccggcattc tgacgggttt gaaaatcaat ttttttacta ccgtctcttt
    25861 ttggtagcac ttttcccaga ctgcagaaaa tgagtggttt tcaagttgtt caataaaacc
    25921 cggagcaact tcaggattaa tatatgtttc gtgatagaaa actttatgtc cttccagcgc
    25981 cccccagccg gtgatgcgat agagttcagt tgatgatgtc actgcaatca gtggcgcgag
    26041 cgtctcggga atatcgctgg tacggctttt ctcggtaaac ccccaggaag gttcccgccc
    26101 ttgctcaatt gcggcccgct gaaagctcgc cgacagctcc gggctgtaat taaatcgcgg
    26161 ctgagtcacg aaccaccctt tacgatcctt acggaagatt ttggattctg cctcgaggtt
    26221 cagcaacgcc tggcgcagcg tcatacgttt aatgcccagc agctctccca gctctctttc
    26281 tgaagggagt tttccacccg gcgtagtgat gccatctttt aaccaccgat ccagtttctc
    26341 ttttgcattt tctacggtgg attgatttcc cgtcattgtt taatcctttt ggtttaaacc
    26401 agatgaggca tcttacgctt acgccaaaaa agaaagcaat taacggcgta tgactttgca
    26461 gataattgtg acaaagctct ctattgacgt aaccacagac ttataaggga gatactcgca
    26521 ttgctgagtg ctcacgacag gcaaaaaaaa acctgccatc gctggcaggt tttttatgac
    26581 taaaaaacga aattaatcgt ctttcggtgc gcggttggcg cgacgacgat cgttttccgt
    26641 caggtgacgt ttacgaatac ggatagaggt cggggtcact tctaccagtt cgtcatcatc
    26701 gatgaactcc agagcttgtt ccagagtcat gcggataggc ggaaccagaa caacggcttc
    26761 gtcagtaccg gaagcacgca tgttggtcag tttcttaccg gtcaggcagt ttacagtcag
    26821 gtcgttagag cggctatgaa taccgataat ctgaccttcg taaacttctg caccgtgacc
    26881 gaggaacagc ttaccgcgat cctgcagacc gaacagcgcg aacgcgaccg ctttaccctg
    26941 accgttagag atcagtacgc cgttctgacg ctgacccact tcacccggac gtacgtcgtc
    27001 gtagtggctg aaggtggagt acagcagacc agtaccggaa gtcatggtca tgaactcaga
    27061 acggaagcca atcagaccac ggcttgggat cacgtagtcg agacgtacgc ggcctttacc
    27121 gtctggattc atgtttttca ggtcgccttt acgttcgccc agcgcctgca ttacagaacc
    27181 ctgatgctgt tcttcaacgt ccagagtcac gttttcatac ggctcttgtt tacgaccgtc
    27241 gatttcacgg aagataactt tcggacggga taccgccagt tcgaaacctt cacgacgcat
    27301 gttttcgatc agaacagaca ggtgcagttc gccacgacca gaaacgcgga acgcatcggc
    27361 gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc agttctttgt tcagacgatc
    27421 caggatctga cgagacgtta cgaacttacc ttctttaccg cagaacggcg aggtgttaac
    27481 gcagaagaac atagaaacgg tcggctcatc aacggagagt gccggcagcg cttcaacgtt
    27541 ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgcca aggcccgtga tcgcaacgat
    27601 atcgccagct tccgccagat cggtttcgat acgttccaga ccgaggtggc ccagcacttt
    27661 accgactttc gcgttgcggg ttttgccttc gctatcgatg atagtgacct gctggttcgg
    27721 cttcacttta ccgcgcttga tgcggccaat gccgataacg ccaacatagc tgttgtaatc
    27781 gagctgagaa atctgcatct ggaacggacc gtcaaggtca acgtccggcg caggaacgtg
    27841 atcaacaatc gcctgataca gcggggtcat gtcttccgcc atatcttcgt ggtccagacc
    27901 cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag tccagctgct cgtcggtcgc
    27961 gtcgaggtta acgaacagat cgaatacctg atccacaacc caatcaggac gcgcgccagg
    28021 gcggtcaact ttgttgataa caacaatcgg cttcaggccg taagcaaacg cttttttggt
    28081 tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca accaccagca gcactgagtc
    28141 taccatggac attacacgtt caacttcacc accgaagtcg gcgtgccccg gggtatcaac
    28201 gatgttgata cggtaatcat tccatttgat agcggtgttt ttcgcgagga tggtaatccc
    28261 acgctctttc tccaaatcgt tggagtccat cacgcgctct tgggtttcgg cacgagagtc
    28321 gaacgtaccg gattgttgga gcagcttgtc taccagggtg gttttaccat ggtctacgtg
    28381 cgcgatgatg gcgatattac gcaatttttc gatcacaact ttgcctcagg cattagaaat
    28441 agcgcgttat tgtacacgga ttaatcgcac tacaaaacag gatcacaaac atcctccgca
    28501 aacaagtatt gcagagtccc tttgtgatcg ctttcacgga gcataaaaag ggttatccaa
    28561 aggtcattgc accaacatgg tgcttaatgt ttccattgaa gcactatatt ggtgcaacat
    28621 tcacatcgtg gtgcagccct tttgcacgat ggtgcgcatg ataacgcctt ttaggggcaa
    28681 tttaaaagtt ggcacagatt tcgctttatc ttttttacgg cgacacggcc aaaataattg
    28741 cagatttcgt taccacgacg accatgacca atccaggaga gttaaagtat gtccgctgaa
    28801 cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgat
    28861 actaaaggta aagaacagca cgtcactatc cctgctcatc aggtgaatgc tgaattcttc
    28921 gaagaaggca aaatgtttga cggctcctcg attggcggct ggaaaggcat taacgagtcc
    28981 gacatggtgc tgatgccaga cgcatccacc gcagtgattg acccgttctt cgccgactcc
    29041 accctgatta tccgttgcga catccttgaa cctggcaccc tgcaaggcta tgaccgtgac
    29101 ccgcgctcca ttgcgaagcg cgccgaagat tacctgcgtt ccactggcat tgccgacacc
    29161 gtactgttcg ggccagaacc tgaattcttc ctgttcgatg acatccgttt cggatcatct
    29221 atctccggtt ctcacgttgc tatcgacgat atcgaaggcg catggaactc ctccacccaa
    29281 tatgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag gcggttactt cccggttccg
    29341 ccggtagact ctgctcagga tattcgttct gaaatgtgtc tggtgatgga acagatgggc
    29401 ctggtggttg aagcccatca ccacgaagta gcgactgctg gtcagaacga agtggctacc
    29461 cgcttcaata ccatgaccaa aaaagctgac gaaattcaga tctacaaata tgttgtgcac
    29521 aacgtagcgc accgcttcgg taaaaccgcg acctttatgc caaaaccgat gttcggtgat
    29581 aacggctccg gtatgcactg ccacatgtct ctgtctaaaa acggcgttaa cctgttcgca
    29641 ggcgacaaat acgcaggtct gtctgagcag gcgctgtact acattggcgg cgtaatcaaa
    29701 cacgctaaag cgattaacgc cctggcaaac ccgaccacca actcttataa gcgtctggtc
    29761 ccgggctatg aagcaccggt aatgctggct tactctgcgc gtaaccgttc tgcgtctatc
    29821 cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg aagtacgttt cccggatccg
    29881 gcagctaacc cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga tggtatcaag
    29941 aacaagatcc atccgggcga agccatggac aaaaacctgt atgacctgcc gccagaagaa
    30001 gcgaaagaga tcccacaggt tgcaggctct ctggaagaag cactgaacga actggatctg
    30061 gaccgcgagt tcctgaaagc cggtggcgtg ttcactgacg aagcaattga tgcgtacatc
    30121 gcactgcgtc gcgaagaaga tgaccgcgtg cgtatgactc cgcatccggt agagtttgag
    30181 ctgtactaca gcgtctaagt gttttagttg ccgtggaaac ttttcgcctg tctctggcag
    30241 gcctgggatc ggtggcaagc acatcacgcc ggatgcgacg caaatgcgtc ttatccggcc
    30301 tacacggtga tgatgtggta ggccggagca ggtgagtcgc tctccaacgt gaagtttgtc
    30361 agctatctgt agcccatctc tgcatgggct tttttctccg tcaattctct gatgcttcgc
    30421 gctttttatc cgtaaaaagc tataatgcac taaaatggtg caacctgttc aggagactgc
    30481 tttatggcaa caggcacgca gcccgatgct gggcagatcc tcaactcgct gattaacagt
    30541 attttgttaa tcgatgacaa cctggcgatc cattacgcca accccgccgc gcaacaactg
    30601 ctcgcccaaa gctcccgcaa attgtttggt acaccgttac cggaactgtt gagctacttc
    30661 tcattaaata tcgagctgat gcaagaaagt ctggaggcgg ggcaaggttt taccgataac
    30721 gaagtgacgc tggtcatcga cgggcgctcg catatccttt ctgtgacggc ccagcgtatg
    30781 ccggacggca tgatcctgct ggagatggct ccgatggata accagcgccg cttaagtcag
    30841 gaacagctac agcacgccca gcaggttgct gcccgtgatt tagtgcgcgg cctggcacat
    30901 gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc agctgctcag caaagcgtta
    30961 cctgacccat cactactcga atataccaaa gtgattatcg aacaggcgga ccggctgcga
    31021 aatctggtcg accgtctgtt ggggccgcag ctgcccggta cgcgcgttac cgaaagtatt
    31081 cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg aactgccgga caacgtgcgg
    31141 ttgattcgtg attacgatcc cagcctgccg gaactggcgc acgacccgga tcaaattgaa
    31201 caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc tggggccgga aggcggtgaa
    31261 atcattctgc gtacccgcac cgcgtttcaa ctgaccttac acggcgagcg ctaccggctg
    31321 gcggcgcgga ttgatgtgga agataacggg ccgggcattc cgcctcattt gcaggatacg
    31381 ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg ggcttggctt atccatcgct
    31441 cgtaatttga ttgatcagca ttcaggcaaa attgaattta ccagttggcc agggcatacc
    31501 gagttctcgg tttacctgcc tatcaggaaa taaaggtgac gtttatgcaa cgagggatag
    31561 tctgggtagt cgatgacgat agttccatcc gttgggtgct tgaacgtgcg ctcgctggag
    31621 cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt actggaggcg ctggcgagca
    31681 aaacgccgga tgtgctgctt tcagatattc gtatgccggg aatggacggg ctggcgctgc
    31741 tcaagcagat taaacagcgc catccaatgc ttccggtcat cattatgacc gcacattccg
    31801 atctggatgc tgccgtcagc gcctatcaac aaggggcgtt tgattatctg cccaaaccgt
    31861 ttgatatcga cgaagcagtg gcgctggttg agcgcgctat cagtcattac caggaacagc
    31921 agcagccgcg taatattcag cttaacggcc caacgaccga tatcatcggc gaagcgccag
    31981 ccatgcagga cgtgttccgt attatcggtc ggctttcgcg ttcttctatt agcgtgctga
    32041 ttaacggcga atccggcacc ggtaaagaac tggtcgctca tgccctgcat cgccacagtc
    32101 cgcgcgccaa agcgccgttt atcgcgctga atatggcagc tatcccaaaa gatttgatcg
    32161 aatcagaact gtttggccac gagaaaggcg cgtttactgg cgcgaatacc attcgtcagg
    32221 ggcgttttga acaggccgat ggcggtacat tattcctcga cgaaattggt gatatgccgc
    32281 tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg tcagttttac cgcgttggcg
    32341 gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc cactcaccag aatctcgaac
    32401 agcgagtgca ggaaggtaag ttccgtgagg atctgttcca ccgcctgaac gttatccgcg
    32461 ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc ccgtctggcg cgccattttt
    32521 tacaggttgc cgcgcacgaa ctgggcgtag aagcgaagtt actgcatccg gaaaccgaag
    32581 ctgctctgac gcgtctggcg tggccaggca acgtgcgcca gctggaaaac acctgccgct
    32641 ggctaacggt gatggccgcc gggcaggaag tgttgattca ggatttgccc ggcgaactgt
    32701 ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc ggacagttgg gcgacgcttc
    32761 ttgcgcagtg ggcagacaga gcgctgcgtt ccggtcatca aaatctgctt tccgaagcgc
    32821 agccagagct ggagcggacg ttactgacga ccgcgttgcg acatacgcag gggcataaac
    32881 aggaagcggc gcggctactc ggctggggcc gcaacaccct gacgcgtaag ttaaaagagc
    32941 tggggatgga gtgattcaca gcttgtgtgt aaagattgat tattgagcgc aaattgctgg
    33001 tattttgcgc tttactgttc cgataagttc agtatgatct tgcccggaaa acggggagag
    33061 tcattatgct ggaatcaata attaatctgg tatcgagtgg cgcagttgac agccacacac
    33121 cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat tgggctgttt agctgaagct
    33181 cgttgtttat gccggatgcg gagaaagtcg taggcctgat aagacgcgac cagcgccgca
    33241 tcaggcatca tgctcagatg tctgatgcgg cgtaaacgcc ttatccggcc tacaaaagca
    33301 ataatttcaa caggttttgt tgcccagtgc atcaggcaat cttgttctca gcacattgat
    33361 ggcgctttcg ttttctactt tgtaaacgaa gcgccattca ctatttaaat cacccgagag
    33421 aactgctgca tccgcgcttt ctggcgcaga taggtatcaa agcacatgca aatgttgcgg
    33481 atcagcaagc gacctttcgc cgtcacctgt attcccttct catccacatc caccagccca
    33541 tcttttgcta acggggcgag cagcttgaga tcttccgcaa agtaatcagc gaagtgcaaa
    33601 tcccactgtt tctcaatagg ggcgtaatcc agacggaagt tgcagatgag cgacttaatc
    33661 acatcgcggc gaatacagtc atcacgcgtt agcgcaatac cacgccacag cgcattgcct
    33721 tgttcatcca cttgctgata gtactgcttc aactctttct ggttctgcgc gtagcagtcg
    33781 ccaatcatgc tgatggcgga aacgcccatc cccagcagat cggtatcgcc ctgagtggtg
    33841 tagccctgga agttacgatg cagcacgcct tcacgctggg ccaccgccag ctcgtcatcc
    33901 ggacgggcaa agtgatccat accgataaac tgatagcccg attgcgtcag gaaggcgatg
    33961 gtttcctgca ggatatcgag tttttgctgc ggactcggca ggtcagcatc tttgattttg
    34021 cgctgagcag caaaaatggt cggcagatgc gcgtagttaa agacactcag acgatcgggg
    34081 ttcagctccg ccacacgttt cagggtaaag gcgaaactct ccggcgtctg tttcggcagg
    34141 ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct cacgcgcatg gttaagcagt
    34201 gcaaagatga actcttcatc ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag
    34261 tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc gtaaatgatc gagtacatcc
    34321 agttcgattt cccgcggatc gacttcgatc gaaatctccg catcggcatt gaactggaag
    34381 ttttcgcgca gcagcttcat caggcggctg atttgcgctt tattcagata cgtcggcgtt
    34441 ccgccgcccc agtgcaattg gctgacgtga cgcccggcga acagcggtgc acgatggacg
    34501 atttcttgct ccagcgcgtc cagatactga tcggccttgt gctgctggcg agtaacaatc
    34561 ttattgcaac cgcagaagta acaaagctta tggcagaacg ggatatgtac gtagagagat
    34621 aatggacgct caggatagcg cgccacggct tgtaaaaacg cctgttcgcc gaagtcttct
    34681 gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc cggaatagtt atatttctgg
    34741 atcagggcca gatcccagtc gatttgctgt acagacatgc tcactccttc cgatggcgtc
    34801 tctggcggct acggcgtacc ggcggatgcc cgtttcgggt catcagccgg ttgcgcagcc
    34861 acttttgtcg ccgcgacaac cgctgtagtt taacgaataa ccacaccaga taacatataa
    34921 ccggaagggt tatcagcaga acgggaaggc ccatggcggc aatccgttag ttgccccgca
    34981 gcagacgcat catgtcttct tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag
    35041 agaggccgag tttctgcatc aactcatcaa tacgatccag tttagcatcc acccaggatt
    35101 gctcttcggc actcagggtt tcgcctgctt ccagacgttc cagcagcgca tccagacgct
    35161 catccgtttc cagtaattcc aactccgcct gcggtgaaag cataggtttc tcactcttcg
    35221 gtttgtgctg tttggtgact ttttcagtca cgcccaatgg aataggggtt ttactgccaa
    35281 tacgtggatc ttttggtgcg ttctggcctt tgctgcctga cgtggtgtta ccgcccgctg
    35341 cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg cttgcggtca cgagcttcct
    35401 gattcagctc ttcgcgcgtt ttgcgacgcg cttttgcatg acctttgctg cgtgatggtg
    35461 aagatggttt cataatggtt cgtcttttag ccgttttatt cagtatagat ttgcggcgga
    35521 atctaacaga aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg
    35581 actcataacc cttaacgggt cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc
    35641 attagccaga acaatccgtg ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt
    35701 cttcctgaca atcctctgtc ttcgcctcct ggcgctcctg accctcatcc tgagtcattg
    35761 ttcctgttag cgtcctcgct ctatgggtgc tactttacgc ctttgcttta aacaaacaat
    35821 caaccgccat cagtatttgc acattttcag agttttatat aaaagcaatt cactgattta
    35881 tatagcttat ttgacatgca tcgtagaatt ttctctgcta tttcgcataa agactatcgg
    35941 caatgtctca caaagcgtat ggcttttact tacaacgctc acgccgaaaa atccgccttt
    36001 tattatggtt caggtataat cgccacaaag ctttgactga cggagacgac cgctttgact
    36061 aatttgaatt atcaacagac gcattttgtg atgagtgcgc ctgatattcg ccacctacct
    36121 tccgataccg gaattgaagt ggcttttgca ggccgttcca acgcaggtaa atccagcgcg
    36181 ctgaacacgc tgactaacca gaaaagcctg gctcgtacct caaaaacccc agggcgcacc
    36241 cagcttatca acctgtttga agtggctgac ggcaagcgtc tggttgactt gcctgggtac
    36301 ggttatgcgg aagtcccgga agagatgaag cgcaaatggc agcgtgcgct cggcgaatac
    36361 ctcgaaaaac gtcagagcct gcaaggtctg gtggtgctaa tggatattcg ccatccgctg
    36421 aaagatttgg atcagcagat gattgagtgg gcggtagaca gcaatatcgc cgttctggtg
    36481 ctgctgacca aagcggacaa actggcaagc ggcgcacgta aagcgcaatt gaatatggtg
    36541 cgtgaagctg tactggcgtt taacggtgat gtgcaggttg aaacgttttc ttcgttgaag
    36601 aaacaaggcg tggacaagct gcggcagaaa ctggatacct ggtttagcga gatgcagcct
    36661 gtagaagaaa cgcaggacgg cgaataattt tcttgcctta atgcttgtgc cggatgtggc
    36721 gtatccggcc cgtaaattca atagattaca attctaccgt tggcctgata aacatagcgc
    36781 atcaggcatt acggatcttt tctttcgccc aataaaaaac gccccagtca ttactgactg
    36841 gggcggctaa aatattcagc caaatccgat tacgtgaagt aaaaggtctg aaagatagaa
    36901 catcttacct ctgtacccta cgcgactaac tttactcttt tttgttcagt tcagaaagca
    36961 ctttttgtaa ttttttttca gttgttgcat aggaatttct aatagccatc acaaaacgtg
    37021 acagcttatg ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg
    37081 atcccagttt tcgccactcc ccacttccac cagcaacggc acatccagac gggtacagtt
    37141 ttccatcagt tgatgaatct gcttcgcgac ggcatcaaca tcatctttat gaacttcaaa
    37201 taccagttca tcgtgtacct gcatgatcat acgtacacgc ggttgctcag cctgtaacca
    37261 cgcatcaacg gcaatcatcg cccgtttgat aatgtcggcg gcggttccct gcattggcgc
    37321 gttaatggct gcacgttcag ccgctgcacg acgagcacca ttgctggatt tgatatccgg
    37381 cagatacaga cggcgtccgt ccagcgtttc aacgtagccc tgctctttcg cctgagcacg
    37441 ggtgcgttcc atatactcca gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt
    37501 ctgcgcttct ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac tcatgccata
    37561 aatcagacca aagttgatcg ctttcgcgct acggcgttgc tcgctggtga cggtttccag
    37621 tggcaaacca aacacttctg ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc
    37681 ggtcagcaag cctttgtcac gcgaaagatg cgccataatg cgcagttcaa tctgcgagta
    37741 gtccgctgag acaatcacat aatcctctgg cgcaataaac gcctggcgga tacgacgacc
    37801 ttcttcgtta cgcaccggaa tgttttgcag gttaggatcg gttgacgata aacgtcccgt
    37861 tgcagttact gcctggtgat aagaggtatg cacacgcccg gttttcgggt tgatcatcag
    37921 cggcagcttg tcggtgtagg tcgatttcag cttcgccaga ccacgatact ccagaatcac
    37981 ttttggcaac ggatagtcca gcgccagttc ttccagtacc tcttccgacg ttgacggcgc
    38041 gccacccggc gttttcttca gcggtttaat gccctgtttt tcaaagagaa tggtttgtaa
    38101 ctgcttggtg gaagaaaggt taaattcctc acctgcaatt tcatgcgctt tcttttccag
    38161 ctcagccaga cgaagggtga gctcttcaga atgattgtgc agcactttcg gatcgatctt
    38221 cacaccgtta cgttcaatgc gtgaaagcac cggcaccagc ggcatttcga tattctcgaa
    38281 gacgttcaac ggccctttgt gtttttgcag atccggccac attttcagat gcaactgcaa
    38341 ggtgacatct gcatcttcgg cggcgtaacg tccggcttct tcgagggcaa tctggttaaa
    38401 ggtcagttga tttttgcctt taccagcaat ctcttcaaaa gtgatggttt tgtgcttcaa
    38461 ccaacgttcc gcgaggctgt ccatatcgtg acgcccggca acgctattga gaatgtagga
    38521 ctccagcatg gtatcaaacg caatcccacg cagttcaatg ccgtagttcg ccagaatacc
    38581 gcgatcgtat ttcaggtttt gcccgacctt cagcgccttt tcatcttcca gcagcggttt
    38641 tagcaactcg agtgcacgct cgcgagagat ttgatcgggc gcatcaagat aatcatgagc
    38701 aaccggaata tatgccgcta cgcctggctc gatagcaaaa gaaagcccga ccaggttagc
    38761 agagatgtta tcaaggctgt cggtttcggt atcaaatgca aataccggcg ctttttccag
    38821 cttcgcaatc cacgctttca gtgtttcttc atcaaggatg gtgacgtagt tgtcataaga
    38881 aatcaccgtt gccgtcactt ctggtgcttc gtctgcaaca ctggtttcct gtggcttcgc
    38941 ggctggtttt gcccctttgg cctgtaacca tttgcccgct tcgacatcag cagtccagcg
    39001 tttgaactca tactttttga acagccccaa caactcttcc gctgccggtt gctgcacttc
    39061 cagttgttca caggtcagct ccagttcaac gtcggtttta atcgtcgcca gctggtatga
    39121 gagataagca acttctttgt tttgctcgag cttcgctgcc attgttttcg cgccacggaa
    39181 gctcaaccca gcaatttttt ctggctcggc atacagcgta tccagtccgc caagaccttg
    39241 cagcaatgcc tgcgcggttt tttcaccgac gcccggtacg ccaggaatgt tatcagagga
    39301 gtcacccatc agcgccagga aatcgatgat cagttctggc ggcacgccgt acttattcac
    39361 cacctcttcc ggtccgagga tggtattcgt catggtattg ataagcgtaa tatttggcgt
    39421 caccagctgc gccatatctt tatcgccagt gctgatcagc accggacgcc cggctttttc
    39481 ggcttcgcgc gccagagtac cgataacgtc gtccgcttct acgccagaaa ccgccagcag
    39541 cggcagtccc atcgctttaa ccatcgcgtg caagggttcg atttgtgcac gcagatcgtc
    39601 cggcattggc gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc
    39661 cttggcgtca aagaccaccg ctgcatgcgt cggtttatat tgcatgatca gactgcgcag
    39721 catgttgagg acaccataca tcgcaccggt cggctcgcct gcgctgttag tcagcggggg
    39781 aaacgcgtga tatgcgcgat aaagataaga tgaaccatct acaaggataa gtggattttg
    39841 ggggatctga accataatgt ccgtgcctgt ttatcagatt atgggtaaag gatgccacag
    39901 acaggatgaa aacatgagtt tttcaccgct tttcgtgaaa atcttttgta gatcttctgg
    39961 atcgctcgca aatcgtcatg tggataactt tgtgcataaa ttatttacat cgcttttaat
    40021 ttattatcat gctttttaca gcatgatgtt tgtctttgtt tttcatgcag ttaataataa
    40081 atccatccct aataattgag ataatgggat tttttctaat gtggatagat atgaattatt
    40141 tttctcctta aggatcatcc gttatttggg tcgttttctg ctaaaatctg cgccttgcgc
    40201 ttctttagcg cactgtctat ggctaacctt ttgaataaat tcattatgac gagaatactc
    40261 gctgcgataa cccttttgct gagtatcgta ttgaccattt tggtcactat tttttgttcc
    40321 gtcccgatca tcattgccgg gattgtaaaa ctattgctac ctgtgccagt catttggcga
    40381 aaggtttcgc gtttttgtga ttttatgatg tattgctggt gtgaaggtct ggcggtatta
    40441 ctgcatctga acccacacct gcaatgggaa gttcacgggc tggaagggtt aagtaagaag
    40501 aactggtatc tgcttatttg taaccaccgt agttgggcag atattgtcgt actgtgcgtg
    40561 ctgtttcgta agcacattcc gatgaataaa tatttcctca agcaacagct ggcctgggtg
    40621 ccatttcttg gcctggcgtg ctggtcgctg gatatgccgt ttatgaagcg ttattcccgc
    40681 gcttatttat tacgtcatcc ggagcgacgt ggtaaagatg ttgaaaccac tcggcgttct
    40741 tgtgaaaagt ttcgcctgca tcccaccacc attgtcaatt tcgttgaagg ctcccgtttc
    40801 acgcaagaaa aacatcagca aacccactcc acttttcaaa acttgttgcc accaaaggcg
    40861 gcaggcattg cgatggcgct aaatgtactg ggtaaacaat tcgataaact gcttaatgtt
    40921 acgctgtgtt atccggacaa taaccgtcag ccgttcttcg atatgttaag cggtaaactg
    40981 acgcggattg tcgtacatgt ggatttacag cctattgccg atgagttaca tggcgactac
    41041 atcaatgaca aaagctttaa gcgccatttt cagcaatggc tgaactcatt gtggcaggaa
    41101 aaagaccggc ttctgacgtc tctcatgtca tcacagcgtc agaataagta agttttactc
    41161 gcctctgaat gagcagaggc gagtgagtat tttaatgaac aaaacgtccg gcacgagaca
    41221 taaattcttc ttcgctcatc tcctgtccgt taaaaacaac tttacccggt gtataacgca
    41281 gctgcagcga ggcggtattg ttgtcaatcg tgattaactg aaacatctga cctaatgtca
    41341 tcatcccgct gatttgttta tcagcctgct tttgcgcttt ttccgcatcc attccttcag
    41401 ataaattaag ctgttttgcg gtttctgtga cgacattaag cggtaacttc acatcaaaat
    41461 tgagcgattt gatatctttg tttgtggatg atgaagattt ggctgggtcg gcaatactga
    41521 tatccagatt ggcatttagt tcgccgagtg cgttcttcca tcttaccggt tgtttaatgg
    41581 tcggctcact tttttgtaat aacggtaaat actctttgaa caatgcagca ttcacttctt
    41641 gcagagcaac ttcatcgttt gctaactcag ggtgtgcagc aagctgcttc tgcatcgcaa
    41701 tgttatactg gatgataaat tggcgcactg ctgagggatc aatagattca gcaatcaatg
    41761 accactcacc actaccgagt tgctgattcc cttttgttaa cttatcaagt tcgtaggtga
    41821 gattgacatt gatataatct ttgtctttaa cgcgatccag cctggttttt gcaacgaaag
    41881 catcgatttg ggcaacgtct tccccgtgat tgataatgtt aatctgatta aatttcgact
    41941 cgctttcacc aaccggaaat ttcttttctt ctaatcgcgc cagagaatca atggtaaaac
    42001 tttttgccgt tactttgttc attggtgata attgcagaac aatatcttcg actttgcctt
    42061 caatatccag gttagacaat gaactaccat caccattaaa agtaaactgg ccctcaccaa
    42121 aagaaaactt tccatactct gcaggggcaa catctaatgt cgtagagaat gagccaccaa
    42181 acgcgaatcg caatgtcgct tccacgggcg atttattttt cgcggcgata aatagcggtt
    42241 gtgtcagttc attattcact aagttcactt ttgccgctgc cagtgctggg atgacattac
    42301 catgcattaa cattgtgatg ggcaacggac cgtgctcaac atccacatca aatacaactt
    42361 tctggcctgg cttgatattg agatcgggtg caccgttatc gaatgtcatt tgcatttgaa
    42421 aacgggaatt aaaaaagcct ttgtcaaaat tttgataact taacgtcata tcgtaggcat
    42481 gttcaccttt ctttttagca tcgttaaaat ctttaataaa tttttcgaca ccaggctgaa
    42541 tttgcgtacc tgtgtaccat gtgccaccac cccagattac ggctaacgca acaataacac
    42601 ctgtagctga cttacgtatc atataaatat tccctgataa aacagcaaaa ctttgaatat
    42661 ccacttatgc tgaaataatg tccagcggca ggatgcatta tcagaatgta tttatattta
    42721 ttaaataata aaaaaagccc gtgaatattc acgggcttta tgtaatttac attgaattat
    42781 tttttctcgg acagatattt cactgtatca gcatactgct gaacaaaaac atccatattg
    42841 ctggtatcca taccctgcgg attcagctga tatttaccgt taacaaacat cgccggaacg
    42901 ccacgcaatt gcacgtcagc tgcagctttt tcctgctgag cgaccagaga tttcaccacg
    42961 aagctgttcc acgccgcgtc gtactcttca cctttaatac ctgcgttgat aaatacatcg
    43021 cggatatcag aagcagaacg aatggtctgg gttttctgta cgccttcaaa cagcggaaca
    43081 gtcactttgt cttccacgcc cagcgccatc gccacagccc atgcctgagt cagatctttg
    43141 cccaggtcac cacccatgaa gttgacgtgg tatttagtca tcttcacgcc ttccggcagt
    43201 tttttcttca cattatcaga aatatgcaga acttcttcaa actgatagca gtgcgggcag
    43261 aagaaagaga aaaactccag cacttgcggc gcgccagcta ccggtttttc cagggtagtg
    43321 tactgtttac catcttcata ctgcgccgcc gatgcgctaa acgctaaaac taaaccagcc
    43381 agcgccagcc aaatcttttt catgatctac tctctccgat taatacatag gtgttaattg
    43441 caaagggggt tcttgtagaa cttttgcctg ttctataaaa gtcgccgtct gtcgtagcca
    43501 gtaatcttcc ccggttaacc acgggaaatt tttcgggaac gcgggatcag cccaacgccg
    43561 cattagccag gcaagataat aaaccaaacg catggcgcgt aaaggttcaa tcagtccgat
    43621 ttcagcggtg tcgaactcgc taaattcttc ataagcttca ataatagttt ccagttgcat
    43681 ccgctgctcg gctttatcgc cattgagcaa catccacaaa tcctgaacgg ctggaccatt
    43741 acgtgcatca tccagatcaa caaacattgg accatcgcgc cagagaatat tcccggcgtg
    43801 gcagtctcca tgtagccgca gaacggtgaa atcttcccgc cagtgtgctg taacggcggc
    43861 aatcagctca tctgtcgctt tcaggaatgc cgctttcaac ccggaaggta tcagtgtagc
    43921 gtcctcaaac agcttgcgtg gctcaatgag atattcgttc aaaccgatgg tcgggcgatg
    43981 gataaaaagc tgtttgcgcc ccgtctggtg catacgccct aaataacgcc caaccgcttc
    44041 catctgatcg atattatcag cttcgaactg gcgaccaccg acgcttggaa aaacagcgaa
    44101 ataaaatccc tgatgattca ataaagtctg accgttaaag gccacaggtg ctgcgaccgg
    44161 aacttcatca tttaccagct gcaacgcaaa ttgatgttct tcgaggattt gatcggctgt
    44221 ccaacgttca gggcgataaa atttgacgac aaaacgtcga cgatcttcgt cctgaaattg
    44281 atagacacgg ttttcatagc tgttaagcgg ggtaagaccg gaatccaccc ggatcccatg
    44341 ctcaaacaga gcgtccatga tggtatccgg gtgtagtgtc tggaaagtaa aagcgctgtt
    44401 attcatccca tcatccggaa aacattacga atgacgaagg atatcatttc acgatgattt
    44461 tggtggcgcc gcttacaagc ttttactctt taattacgcc gcgagcgcgt aacagcgccg
    44521 ttttgaagtc ttcttcataa tctttctgca aacccgggat caccgcatct ttcgcggaat
    44581 cacgcatttt caggtgatag atcagaatgt catccgtcaa atccgccagt tcgccgtcaa
    44641 aacctgactc tttcgccagt ttctgcaaaa attgcagcag gttaaggtct ggctcttttt
    44701 gccaggctgg ctggaggagt tcaataactt cattcagacg tttacatttc atagtgatgc
    44761 tccttactct tgagacagac acgttagcag ggtcaatccc acaataaaag aggcgatatc
    44821 ggtgaatctg atgacgacga taacaggcgt tgtgctggca ggcggtaaag ccagacgaat
    44881 gggcggcgta gataaaggat tgcttgaatt aaacggcaaa ccattatggc aacatgtcgc
    44941 tgacgcgctt atgacgcagc tctctcacgt cgtggttaat gctaatcgtc atcaggaaat
    45001 ctatcaggca agcggtctga aagtgattga agattcactg gcggattacc caggccctct
    45061 ggcaggaatg ctttcagtaa tgcagcagga agctggtgag tggtttttgt tttgcccgtg
    45121 cgatacgcct tacattcccc ctgatttagc agccaggctt aatcatcagc gcaaagatgc
    45181 gcctgtcgtg tgggtccatg acggtgaacg cgatcacccg actattgctc tggtaaaccg
    45241 cgctattgag cctttattac tggaatatct gcaagcagga gaacgccggg taatggcatt
    45301 tatgcgtctg gctggcggtc atgcggttga tttcagcgat cataaagatg catttgttaa
    45361 cgtgaatacg ccagaggagc ttgcccgatg gcaggaaaaa cgatgatacc gttactcgcc
    45421 tttgccgcgt ggagtggtac cggaaaaact acgcttttga aaaaactgat cccggtatta
    45481 tgcgccagag ggatccgtcc agggctgatt aagcatacgc accatgatat ggatgttgat
    45541 aagccaggca aagatagcta tgagctgcgc aaggctggcg cggcacaaac catcgttgcc
    45601 agccagcaac gatgggcctt gatgacggaa acaccagacg aagaagagct ggatctacag
    45661 tttctcgcaa gtcgaatgga tacctcaaag ctggatttga ttctggtcga agggtttaag
    45721 catgaagaga tcgcaaagat tgtgctgttt cgcgatggag ccggacatcg acctgaagaa
    45781 ttagtgatag acaggcatgt tattgctgta gccagtgatg tgccgcttaa tcttgatgtc
    45841 gcattactgg atattaatga tgttgagggg ctggctgatt ttgtggtgga gtggatgcaa
    45901 aagcagaacg gataagcaag agtaggctgg ataagacgcg ccagcgtcgc atccggcatt
    45961 tttttctgcg ccaatgcaaa aaggccatcc tgacggatgg ccttctgct
//
LOCUS       DAZFZC010000031        46001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFZC010000031 DAZFZC010000000
VERSION     DAZFZC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.031"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..122)
                     /locus_tag="ACHKXP_001970"
     CDS             complement(<1..122)
                     /locus_tag="ACHKXP_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7646988.1"
                     /translation="MLTGDSYKDVKFMLRIFIPTSNGKISRRRYIFSFILINFI"
     gene            complement(181..1071)
                     /gene="rarD"
                     /locus_tag="ACHKXP_001971"
     CDS             complement(181..1071)
                     /gene="rarD"
                     /locus_tag="ACHKXP_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO7646989.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLSFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1123..1590)
                     /gene="yigI"
                     /locus_tag="ACHKXP_001972"
     CDS             complement(1123..1590)
                     /gene="yigI"
                     /locus_tag="ACHKXP_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO7646990.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1755..2624
                     /gene="pldA"
                     /locus_tag="ACHKXP_001973"
     CDS             1755..2624
                     /gene="pldA"
                     /locus_tag="ACHKXP_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO7646991.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2757..4586
                     /gene="recQ"
                     /locus_tag="ACHKXP_001974"
     CDS             2757..4586
                     /gene="recQ"
                     /locus_tag="ACHKXP_001974"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO7646992.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4650..5270
                     /gene="rhtC"
                     /locus_tag="ACHKXP_001975"
     CDS             4650..5270
                     /gene="rhtC"
                     /locus_tag="ACHKXP_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO7646993.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5332..5952)
                     /gene="rhtB"
                     /locus_tag="ACHKXP_001976"
     CDS             complement(5332..5952)
                     /gene="rhtB"
                     /locus_tag="ACHKXP_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO7646994.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6063..7085
                     /gene="pldB"
                     /locus_tag="ACHKXP_001977"
     CDS             6063..7085
                     /gene="pldB"
                     /locus_tag="ACHKXP_001977"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO7646995.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7093..7893
                     /gene="yigL"
                     /locus_tag="ACHKXP_001978"
     CDS             7093..7893
                     /gene="yigL"
                     /locus_tag="ACHKXP_001978"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO7646996.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFQYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7969..8868
                     /gene="bioP"
                     /locus_tag="ACHKXP_001979"
     CDS             7969..8868
                     /gene="bioP"
                     /locus_tag="ACHKXP_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO7646997.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8756..9709)
                     /gene="metR"
                     /locus_tag="ACHKXP_001980"
     CDS             complement(8756..9709)
                     /gene="metR"
                     /locus_tag="ACHKXP_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO7646998.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9946..12207
                     /gene="metE"
                     /locus_tag="ACHKXP_001981"
     CDS             9946..12207
                     /gene="metE"
                     /locus_tag="ACHKXP_001981"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO7646999.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTATLAEWSVPIQARRHSTRVHNPAV
                     EKRLATITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12301..13197)
                     /locus_tag="ACHKXP_001982"
     CDS             complement(12301..13197)
                     /locus_tag="ACHKXP_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7647000.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLIFCS"
     gene            complement(13275..14498)
                     /locus_tag="ACHKXP_001983"
     CDS             complement(13275..14498)
                     /locus_tag="ACHKXP_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO7647001.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSSSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFTVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(14524..14913)
                     /locus_tag="ACHKXP_001984"
     CDS             complement(14524..14913)
                     /locus_tag="ACHKXP_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HFO7647002.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(14930..15886)
                     /gene="arcC"
                     /locus_tag="ACHKXP_001985"
     CDS             complement(14930..15886)
                     /gene="arcC"
                     /locus_tag="ACHKXP_001985"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7647003.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATTQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(15879..17303)
                     /locus_tag="ACHKXP_001986"
     CDS             complement(15879..17303)
                     /locus_tag="ACHKXP_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7647004.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLMDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(17300..18859)
                     /gene="fdrA"
                     /locus_tag="ACHKXP_001987"
     CDS             complement(17300..18859)
                     /gene="fdrA"
                     /locus_tag="ACHKXP_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7647005.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALVLAQQRLTSKPDDETDSGIKSPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPTIETEISCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGIIIASSNAQAALWASAVA
                     QTQLQKKGLNA"
     gene            complement(18954..19814)
                     /locus_tag="ACHKXP_001988"
     CDS             complement(18954..19814)
                     /locus_tag="ACHKXP_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7647006.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAVMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(19820..20487)
                     /locus_tag="ACHKXP_001989"
                     /pseudo
     CDS             complement(19820..20487)
                     /locus_tag="ACHKXP_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
     gene            complement(20744..21553)
                     /gene="ysgA"
                     /locus_tag="ACHKXP_001990"
     CDS             complement(20744..21553)
                     /gene="ysgA"
                     /locus_tag="ACHKXP_001990"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO7647007.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            21815..22579
                     /gene="udp"
                     /locus_tag="ACHKXP_001991"
     CDS             21815..22579
                     /gene="udp"
                     /locus_tag="ACHKXP_001991"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO7647008.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(22613..24103)
                     /locus_tag="ACHKXP_001992"
     CDS             complement(22613..24103)
                     /locus_tag="ACHKXP_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFO7647009.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(24116..24622)
                     /locus_tag="ACHKXP_001993"
     CDS             complement(24116..24622)
                     /locus_tag="ACHKXP_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFO7647010.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(24639..25613)
                     /locus_tag="ACHKXP_001994"
     CDS             complement(24639..25613)
                     /locus_tag="ACHKXP_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFO7647011.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRSDAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKG
                     GKLKTLVVMADERMETMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(25638..26267)
                     /locus_tag="ACHKXP_001995"
     CDS             complement(25638..26267)
                     /locus_tag="ACHKXP_001995"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO7647012.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(26257..27261)
                     /locus_tag="ACHKXP_001996"
     CDS             complement(26257..27261)
                     /locus_tag="ACHKXP_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO7647013.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFNHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(27285..28052)
                     /locus_tag="ACHKXP_001997"
     CDS             complement(27285..28052)
                     /locus_tag="ACHKXP_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO7647014.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            28269..29696
                     /gene="rmuC"
                     /locus_tag="ACHKXP_001998"
     CDS             28269..29696
                     /gene="rmuC"
                     /locus_tag="ACHKXP_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO7647015.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQFAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            29791..30546
                     /gene="ubiE"
                     /locus_tag="ACHKXP_001999"
     CDS             29791..30546
                     /gene="ubiE"
                     /locus_tag="ACHKXP_001999"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO7647016.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            30560..31165
                     /gene="ubiJ"
                     /locus_tag="ACHKXP_002000"
     CDS             30560..31165
                     /gene="ubiJ"
                     /locus_tag="ACHKXP_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO7647017.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            31162..32802
                     /gene="ubiB"
                     /locus_tag="ACHKXP_002001"
     CDS             31162..32802
                     /gene="ubiB"
                     /locus_tag="ACHKXP_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO7647018.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            32881..33150
                     /gene="tatA"
                     /locus_tag="ACHKXP_002002"
     CDS             32881..33150
                     /gene="tatA"
                     /locus_tag="ACHKXP_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO7647019.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            33154..33669
                     /gene="tatB"
                     /locus_tag="ACHKXP_002003"
     CDS             33154..33669
                     /gene="tatB"
                     /locus_tag="ACHKXP_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO7647020.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            33672..34448
                     /gene="tatC"
                     /locus_tag="ACHKXP_002004"
     CDS             33672..34448
                     /gene="tatC"
                     /locus_tag="ACHKXP_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO7647021.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            34490..35272
                     /gene="tatD"
                     /locus_tag="ACHKXP_002005"
     CDS             34490..35272
                     /gene="tatD"
                     /locus_tag="ACHKXP_002005"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO7647022.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVACAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(35269..35757)
                     /gene="rfaH"
                     /locus_tag="ACHKXP_002006"
     CDS             complement(35269..35757)
                     /gene="rfaH"
                     /locus_tag="ACHKXP_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO7647023.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            35924..37417
                     /gene="ubiD"
                     /locus_tag="ACHKXP_002007"
     CDS             35924..37417
                     /gene="ubiD"
                     /locus_tag="ACHKXP_002007"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO7647024.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWLGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            37463..38164
                     /gene="fre"
                     /locus_tag="ACHKXP_002008"
     CDS             37463..38164
                     /gene="fre"
                     /locus_tag="ACHKXP_002008"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO7647025.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(38447..39610)
                     /gene="fadA"
                     /locus_tag="ACHKXP_002009"
     CDS             complement(38447..39610)
                     /gene="fadA"
                     /locus_tag="ACHKXP_002009"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO7647026.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEGLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGMVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(39620..41809)
                     /gene="fadB"
                     /locus_tag="ACHKXP_002010"
     CDS             complement(39620..41809)
                     /gene="fadB"
                     /locus_tag="ACHKXP_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO7647027.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            41999..43330
                     /gene="pepQ"
                     /locus_tag="ACHKXP_002011"
     CDS             41999..43330
                     /gene="pepQ"
                     /locus_tag="ACHKXP_002011"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO7647028.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLDIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            43330..43944
                     /gene="yigZ"
                     /locus_tag="ACHKXP_002012"
     CDS             43330..43944
                     /gene="yigZ"
                     /locus_tag="ACHKXP_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO7647029.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            43983..45434
                     /gene="trkH"
                     /locus_tag="ACHKXP_002013"
     CDS             43983..45434
                     /gene="trkH"
                     /locus_tag="ACHKXP_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO7647030.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            45446..45991
                     /gene="hemG"
                     /locus_tag="ACHKXP_002014"
     CDS             45446..45991
                     /gene="hemG"
                     /locus_tag="ACHKXP_002014"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO7647031.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT        10843 a        11779 c        12358 g        11021 t
ORIGIN      
        1 aaaatgaaat ttattaaaat aaatgaaaat atataacgac gtctggaaat cttaccgtta
       61 gatgttggga taaatatacg taacataaat tttacatcct tgtatgagtc tccggtcagc
      121 atggcaatat gcccactctc atgcaagagt cggcatattt tttcagaata tatttatttt
      181 ttatttggac gttctacgct gcgtataaat cgcatccatc acaaaaattg ccagcgccac
      241 ccaaataaag gcgaaagtca ccatcttatc ggcacccggt ttttcaccat aaaacgtcac
      301 agccagcagg aacatcagcg tcgggccaat gtactggaaa aagcttaacg ttgagagacg
      361 caagcgcgtg gcagcggcgg taaaacacaa cagcggtacg gtagtgacaa taccggcggc
      421 gatcagcagt aaattcagcg acatcgggtt ttgccccata tggctggttg agctgtcggc
      481 aatagcaaac aggtaaattg ccgccacggg cagcagccac atggtttcga ttaacatgcc
      541 ggtttgcgct tcaacggcaa tcttcttgcg taccagaccg tagaaggcaa aactaaatgc
      601 cagtcccagc gcgataatag gtagcgaacc aaaagtccac agctggacta acacgccaca
      661 tatcgccaga atcaccgcca gccattgcat ccggcggaat cgctcgccga ggaaaatcat
      721 ccccagcaca atgttcacca gcgggttaat aaagtaacca aggctcgctt ccagcatatg
      781 gtgattgttc accgcccaga taaacagtag ccagttgcca ccaatcagca cggcagagac
      841 tgccagcata aaaattttct gtggcgtctg aatcagcgtt tttaaatagg accactggcg
      901 gcaaatgctc atcagcacca ccataaagaa aaacgaccag atcacgcgat gcgtcaggat
      961 ttcatcggcg ggcacgtagt aaatcaactt gaagtacgct ggcgctatac cccaaataaa
     1021 ataagcggca agagcgagta atacgccctg ccgcgtttgt tttgcatcca tcgggaatac
     1081 tcatttttaa ttggtaacag cagtttacct gcttttatgt cttcaaccta ccatataggt
     1141 ggcggtggca ctggcaatat aaagctgttc ttcattgtgt aattcaacgc gggcgacggc
     1201 gactttattg cctgcacgca acaggctact agtagcagta aaacgctcgc ccctgcctgg
     1261 gcgcagataa tcaacgcgaa gatcaatggt ccccatccgc gatagccgct ggcgtagttc
     1321 atcttcactg atggtttcgt ggcgggttaa ggtacttccc acgcacacca gaccggcggc
     1381 gacatccagc gccgacgcaa tgaccccgcc gtgcaaaatg ctttgcgccc agttgcccac
     1441 catcattggc tgatttttaa aggccagctg tgcgaactct ttttcgtaac gctccagttc
     1501 catccccaat gcgcggttaa atggcatgtg ataaacaaac atctcaccca ctaatttcag
     1561 ggcttgttca gcggtcagta cggcagacat atcatcctta cacttcattg gttaatgaaa
     1621 tgttgatttt atgcttcttt gttgttggtt tctactttag gaagggataa ctaacggcta
     1681 tggagttaag tatgtaaaat agcccgcaga aaaatattca ccttatcaat aattcgttac
     1741 ggagaacacg accgatgcgg actctgcagg gctggttgtt gccggtgttt atgttgccta
     1801 tggcagtata tgcacaagag gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg
     1861 gcagtattat cgccaatatg ctgcaggagc atgacaatcc gttcacgctc tatccttatg
     1921 acaccaacta cctcatttac acccaaacca gcgatctgaa taaagaagcg attgccagtt
     1981 acgactgggc ggaaaatgcg cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc
     2041 cgctgtggcg tgggatttta ggcccgaact cggtgttggg tgcgtcttat acgcaaaaat
     2101 cctggtggca actgtccaat agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac
     2161 cgcaattgtt cctcggtttt gccaccgatt accgttttgc aggttggacg ctgcgcgatg
     2221 tggagatggg gtataaccac gactctaacg ggcgttccga cccgacctcc cgcagctgga
     2281 accgccttta tactcgcctg atggcagaaa acggtaactg gctggtagaa gtgaagccgt
     2341 ggtatgtggt gggtaatact gacgataacc cggatatcac caaatatatg ggttactacc
     2401 agcttaaaat cggctatcac ctcggtgatg cggtgctcag tgcgaaagga cagtacaact
     2461 ggaacaccgg ctacggcggc gcggagttag gcttaagtta cccgatcacc aaacatgtgc
     2521 gcctttatac tcaggtttac agcggctatg gcgaatcgct catcgactat aacttcaacc
     2581 agacccgtgt cggtgtgggg gttatgctaa acgatttgtt ttgatgaacg gttgagtggt
     2641 tggcaaatct ggaacccagc atccaggatt accctctcag agactaaaag cattgcagtt
     2701 tctcgcgcag gcgctgaaaa tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg
     2761 cgcaggcgga agtgttgaat ctggagtccg gagctaaaca ggttttacaa gaaacctttg
     2821 gctaccaaca gtttcgcccc ggccaggaag aaattatcga cactgtgctt tccggtcgcg
     2881 attgcctggt cgtcatgccc accggtggcg gaaaatccct ttgctatcaa attcctgcct
     2941 tattgctaaa cggccttacc gtggttgttt caccgctgat ttcgttgatg aaagatcagg
     3001 tggatcaact gcaagccaac ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg
     3061 aacagcaact tgaagtgatg acaggctgcc gcaccgggca aattcgctta ctgtatatcg
     3121 ccccggaacg cctgatgctg gataactttc ttgagcatct ggcgcactgg aatccggtgt
     3181 tattagccgt cgatgaagcg cactgtatct cccaatgggg ccacgatttc cgcccggaat
     3241 atgccgcgct cggtcagttg cgccagcggt tcccgacgct gccgtttatg gcgctgaccg
     3301 ccacagccga cgacaccacg cgccaggata tcgtgcgcct gctggggctg aacgatccgc
     3361 tgattcaaat cagcagtttt gaccgtccga atattcgcta catgctgatg gagaagttca
     3421 aaccgctcga tcagttgatg cgctacgtgc aggaacagcg cggtaagtca ggcattatct
     3481 actgcaacag ccgcgcgaaa gtagaagaca ccgctgcgcg cctgcaaagc aagggaatta
     3541 gcgcggcggc ctatcatgcc gggctggaaa ataatgttcg cgccgatgtg caggaaaaat
     3601 tccagcgcga tgacctgcaa attgtggtgg cgacggtggc gttcggcatg ggcatcaata
     3661 aaccaaacgt tcgcttcgtg gtccactttg atattccgcg caatatcgaa tcctattatc
     3721 aggaaaccgg acgcgccggg cgtgatggcc tgcccgcgga agcgatgctg ttttacgatc
     3781 cggctgatat ggcgtggctg cgccgttgtc tggaagagaa gccgcagggg cagttgcagg
     3841 atatcgagcg ccacaaactc aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc
     3901 gtctggtatt gctgaactat tttggcgaag ggcgtcagga gccgtgcggg aactgcgata
     3961 tctgcctcga tccgccgaaa cagtacgacg gttcaaccga tgctcagatt gccctttcca
     4021 ccattggtcg tgtgaatcag cggtttggga tgggttatgt ggtggaagtg attcgtggtg
     4081 ctaataacca gcgtatccgc gactatggtc atgacaaact gaaagtctat ggcatgggcc
     4141 gtgataaaag ccatgaacat tgggtgagcg tgatccgcca gctgattcac ctcggcctgg
     4201 tgacgcaaaa tattgcccag cattctgccc tacaactgac agaggccgcg cgcccggtgc
     4261 tgcgcggcga atcctctttg caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag
     4321 cgatgcagaa atcgttcggc ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac
     4381 tgcgtaaatc gatagccgat gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa
     4441 ccttgattga gatggctgaa cagatgccga tcaccgccag cgaaatgctc agcgttaacg
     4501 gcgttgggat gcgcaagctg gaacgctttg gcaaaccgtt tatggcgctg attcgtgcgc
     4561 atgttgatgg cgatgacgaa gagtagtcag cagcataaaa aagtgccagt atgaagactc
     4621 cgtaaacgtt tcccccgcga gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg
     4681 tgcacattgt ggcgcttatg agccccggtc ccgatttctt ttttgtctct cagaccgctg
     4741 tcagtcgttc ccgtaaagaa gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg
     4801 tttgggctgg gattgcgctg cttggcctgc atttgattat cgaaaaaatg gcctggctgc
     4861 atacgctgat tatggtgggc ggtggcctgt atctctgctg gatgggttac cagatgctac
     4921 gcggtgcact gaaaaaagag gcggtttctg cacctgcgcc acaggtcgag ctggcgaaaa
     4981 gtgggcgcag tttcctgaaa ggtttactga ccaatctcgc taatccgaaa gcgattatct
     5041 actttggctc ggtgttctca ttgtttgtcg gtgataacgt tggcactacc gcgcgctggg
     5101 gcatttttgc gctgatcatt gtcgaaacgc tggcgtggtt taccgtcgtt gccagcctgt
     5161 ttgccctgcc gcaaatgcgc cgtggttatc aacgtctggc gaagtggatt gatggttttg
     5221 ccggggcgtt atttgccgga tttggcattc atttgattat ttcgcggtga tgccagacgc
     5281 gtcttcagag taagtcggat aaggcgttta cgccgcatcc gacattattt ttcacgcatg
     5341 cctcgccgat gctaacagcg ctcccaccag cataaacaac gagccgaaaa tcttattcag
     5401 cgccttcatc tgctttggtc ctttaatcca tagagcaatc cgttgagcaa gggtggcgta
     5461 accgatcatc acaataatat cgaccacaat agtggtgacg ccgagcacga tatactgcat
     5521 cagttgcggc tgttgcggca tgatgaattg cggaaatagc gccgccagaa acacaatact
     5581 tttgggattg gtgagattca caaaaactgc gcgctggaac aaatgtcgac gcgattgagt
     5641 agaggccagc gatttaaggt caattgcacc agcggcgcgc cactgctgga ttcccagcca
     5701 aatcaagtaa gccgcgcctg cccacttcaa cacttcaaac gcaatcactg agcgggaaaa
     5761 tagcgtcccc aaccccacgc caaccagcac aatatgaatc gccagtccgg tctgaagccc
     5821 agcaatagac gccaccgcgc cgcgataacc gtggttgagc gaggtggtca tagtgttgat
     5881 tgcaccagag cctggcgaca ggcttaaaat gatcgatgtc agcaggtagg caaaccacca
     5941 ttctaaggtc atgatgaact cccggtgtgt ctatttttgt gccacaatac gctactgtcg
     6001 cagcgttgtg tcaggcacgc taaaaaaaac gattttacgt ggtttaagag gcagattacc
     6061 cgatgtttca gcagcaaaaa gactgggaaa caagagaaaa cgcgtttgct gcttttacca
     6121 tgggaccgct gactgatttc tggcgtcagc gtgatgaagc agagtttact ggtgtggatg
     6181 acattccggt gcgctttgtc cgttttcgcg cacagcacca tgaccgggtg gtagtcatct
     6241 gcccggggcg tattgagagc tacgtaaaat atgcggaact ggcctatgac ctgttccatt
     6301 tggggtttga tgtcttaatc atcgaccatc gcgggcaggg acgttccggt cgcctgttag
     6361 ccgatccgca tctcgggcat gttaatcgct ttaatgatta tgttgatgat ctggcggcat
     6421 tctggcagca ggaggttcag cccggtccgt ggcgtaaacg ctatatactg gcacattcga
     6481 tgggcggtgc gatctccaca ttatttctgc aacgccatcc aggtgtatgt gacgccattg
     6541 cgctaactgc gccaatgttt gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga
     6601 tcctcaactg ggccgaagcg catccacgtt tccgtgatgg ctatgcaata ggcaccgggc
     6661 gctggcgcgc gttgccgttt gctatcaacg tactgaccca cagcagacag cgatatcgac
     6721 gtaacttacg cttctatgct gatgacccaa cgattcgcgt cggtgggccg acctaccatt
     6781 gggtacgcga aagtattctg gctggcgaac aggtgttagc cggtgcgggt gatgacgcca
     6841 cgccaacgct tctcttgcag gctgaagagg aacgcgtggt ggataaccgc atgcatgacc
     6901 gtttttgtga actccgcacc gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa
     6961 ttaaaggtgc ttaccatgag atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc
     7021 acgccatcgt tgattttttc aacaggcata actcacccag cggaaaccgc tctacagagg
     7081 tttaaatttc ttatgtacca ggttgttgcg tctgatttag atggcacgtt actttctccc
     7141 gaccatacgt tatcccctta cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc
     7201 aactttgtgt ttgcgaccgg tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg
     7261 gagattaagt cttacatgat tacctccaat ggtgcgcgcg ttcacgatct ggatggtaat
     7321 ctgatttttg ctcataacct ggatcgcgac attgccagcg atctgtttgg cgtagtcaac
     7381 gacaatccgg acatcattac taacgtttat cgcgacgacg aatggtttat gaatcgccat
     7441 cgcccggaag agatgcgctt ttttaaagaa gcggtgttcc aatatgcgct gtatgagcct
     7501 ggattactgg agccggaagg cgtcagcaaa gtgttcttca cctgcgattc ccatgaacaa
     7561 ctgctgccgc tggagcaggc gattaacgct cgttggggcg atcgcgtcaa cgtcagtttc
     7621 tctaccttaa cctgtctgga agtgatggcg ggcggcgttt caaaaggcca tgcgctggaa
     7681 gcggtggcga agaaactggg ctacagcctg aaggattgta ttgcgtttgg tgacgggatg
     7741 aacgacgccg aaatgctgtc gatggcgggg aaaggctgca ttatgggcag tgcgcaccag
     7801 cgtctgaaag accttcatcc cgagctggaa gtgattggta ctaatgccga cgacgcggtg
     7861 ccgcattatc tgcgtaaact ctatttatcg taatcgttct ttatttggtc agttgtcaac
     7921 ctgatacttc gctacaatgg atacccgtta atcaaagagt tttccattgt ggcgctactt
     7981 atcatcacca cgattctgtg ggccttctcc tttagctttt atggcgagta ccttgcgggg
     8041 cacgtcgata gctattttgc ggtgctggtg cgcgttggcc tggcggcact cgtttttctg
     8101 ccgtttctgc gtacccgtgg caatagcctg aaaacggtcg gcctgtatat gctggtgggc
     8161 gcgatgcagc ttggcgtgat gtatatgctg agtttccgcg cttatctcta cctgacggtt
     8221 tccgagctgc tgctgttcac cgtgctgacg ccgctctaca tcacgctgat ttatgacatc
     8281 atgagtaagc gccgtctgcg ctggggctat gcctttagcg ccttgctggc ggtgattggt
     8341 gccgggatta ttcgctatga tcaggtcacc gaccatttct ggactggctt gctgctggtg
     8401 caactctcca atatcacttt tgccattggc atggtgggtt acaaacgcct gatggaaact
     8461 cgcccgatgc cacagcataa cgcctttgcg tggttctatc ttggcgcgtt tctggtggca
     8521 gtgattgcat ggttcttgct gggaaatgcg cagaaaatgc cgcaaaccac gttgcagtgg
     8581 ggcattctgg tgtttcttgg cgtggtggct tccgggattg gctactttat gtggaactac
     8641 ggcgcgacgc aggtggacgc cggaacgctg ggcattatga ataatatgca cgttccggca
     8701 gggctgctgg taaacctggc tatctggcac caacagccgc actggccaac gtttattaca
     8761 ggcgcgctgg tgatcctggc ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct
     8821 tcacaaacgg cagatgatcg caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa
     8881 ctggctgacg ctgctcgcca tcgcgcacgg cggcgtacag tcggctccac aagccttcgc
     8941 ccagggtttt ggtcaccacc agaccctggc gctcaaaact ctctactacc caatgcggta
     9001 gcgcggcaat acccatccgc gcggcaacca tctgaatcaa caataaggtg ttatcgacgc
     9061 ttttcagtga cgggctgacg cctgccggct gaagaaaatg ccgccagaca tccagtcgac
     9121 tacgctgcac cggataaatt aatagcgtct cgctggcgag atcttccggt gtaatacgcg
     9181 tttttgccgc cagtggatgg tcaggtgcta acaccaggcg tacttcataa tcgaacatcg
     9241 gcgaataatg caggccactg cgcggtagga tatcggaggt catcaccaga tccagctcgc
     9301 cctgttgtaa agcaggctgc gggtcaaacg tcacgcccga tttaaagtcc atctctacct
     9361 gcggccagtt cttatggaaa ttttctaacg cgggtgtcag ccactgaata cagctatggc
     9421 actcaatggc aatacgcaga cgcgtctgct gcggttcatt gcaggcttgc agtgcctggc
     9481 taatttgcgg cagcacctgg tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc
     9541 gaagcggctg gctcttacgc acaaatagcc ggaagccaag gcgttgttcc agatcgctaa
     9601 actggtgaga cagggcggat tgcgtctgat gcaacgtcgc cgcagcggct gcgagcgagc
     9661 cgcagttccg caacgcttgt agcgttttca ggtgttttac ttcgatcatg aaagtccttc
     9721 acttcggcat gaataatttg cgcttgagga atatacagta accgccaatt atggatgtgt
     9781 aaacatctgg acggctaaaa tccttcgtct tttaaattta tggtgcgttg gctgcgtttc
     9841 tccaccccgg tcacttactt cagtaagctc ccggggatga ataaacttgc cgccttccct
     9901 aaattcaaaa tccataggat ttacatataa ttagaggaag aaaaaatgac aatattgaat
     9961 cacaccctcg gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt
    10021 tattgggcgg ggaactccac gcgtgaagaa ctgctggcgg tagggcgtga attgcgtgct
    10081 cgtcactggg atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc
    10141 tggtacgatc atgtactgac caccagtctg cttctgggta acgttccggc gcgtcatcag
    10201 aacaaagatg gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg
    10261 actggcgaac ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac
    10321 atggtgccgg agttcgttaa aggccaacag ttcaaactga cctggacgca gctgctggac
    10381 gaagtggacg aggcgctggc gctgggccac aaggtgaaac ctgtgctgct ggggccggtt
    10441 acctggctgt ggctggggaa agtgaaaggt gaacaatttg accgcctgag cctgctgaac
    10501 gacattctgc cggtttatca gcaagtgctg gcagaactgg caaaacgcgg cattgagtgg
    10561 gtacagatcg acgaacccgc gctggtactg gaactaccgc aggcgtggct ggacgcatac
    10621 aaacccgctt acgacgcgct ccagggccag gtgaaactgc ttctgaccac ctattttgaa
    10681 ggcgtaacgc caaacctcga cacgattact gcgctgcctg ttcaggggct gcatgtggac
    10741 cttgtacatg gtaaagatga cgttgctgaa ctgcacaagc gcctgccttc tgactggttg
    10801 ctgtctgcgg gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac cgagaaatat
    10861 gcgcaaatta aggacattgt cggcaaacgc gatttgtggg tggcatcttc ctgctcactg
    10921 ctgcacagcc ccatcgacct gagcgtggaa acgcgtcttg atgcggaagt gaaaagctgg
    10981 tttgccttcg ccctacaaaa atgccatgaa ctggcactgc tgcgcgatgc gctgaacagc
    11041 ggtgacacgg caactctggc agagtggagc gtcccgattc aggcgcgtcg tcactctact
    11101 cgtgtacata atccggcggt agaaaagcgt ctggcgacga tcaccgccca ggacagccag
    11161 cgtgcgaatg tctatgaagt gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg
    11221 tggccgacca ccacgattgg ttccttcccg caaaccacgg aaattcgtac cctgcgtctg
    11281 gatttcaaaa agggtaatct cgacgccaac aactaccgca cgggcattgc ggaacatatc
    11341 aggcaggcca ttgttgagca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc
    11401 gagcgtaatg acatggtgga atactttggc gagcacctcg acgggtttgt ctttacgcaa
    11461 aacggttggg tacagagcta tggttcccgc tgcgtgaagc caccgattgt tattggtgac
    11521 gttagccgcc cggcaccgat taccgtggag tgggcgaagt atgcgcaatc gctgaccgac
    11581 aaaccggtga aagggatgct gacggggccg gtgaccatac tctgctggtc gttcccgcgt
    11641 gaagatgtca gccgtgaaac catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg
    11701 gccgatctgg aagccgctgg aattggcatc atccagattg acgaaccggc gctgcgcgaa
    11761 ggtttaccgc tgcgtcgtag cgactgggat gcgtatctcc agtggggcgt agaggccttc
    11821 cgtatcaacg ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc
    11881 gagttcaacg acatcatgga ttcgattgcg gcgctggacg cagacgtcat caccatcgaa
    11941 acctcgcgtt ccgacatgga gttgctggag tcgtttgaag agtttgatta tccaaatgaa
    12001 atcggtcctg gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa
    12061 gccttgctga agaaagcggc aaaacgcatt ccggcagagc gtctgtgggt caacccggac
    12121 tgtggcctga aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag
    12181 gcggcgcaga atttgcgtcg gggatgatga gtattaacga gtcattgtaa attgcagcac
    12241 aataatggct tccaacaccg gaagccattt tgctatgtat gccaggtatg atgtggatct
    12301 tcaggaacag aaaattaatt gcggatttct taaatattct atgcaccact gacaggcttt
    12361 gcccatacca tcggaacgag cggcaagaaa taacttggtg gcgtgtttat gttcgcgcat
    12421 tggcttttta agcaatttac cgctgttcaa taacggcaat gccagatgat gtggaatata
    12481 accaataccg acgttttgtt ctattaaggc gattgccgta gcgaagtcag gaacaaaaat
    12541 gggtttttgt ccttccaaca accaggcttg catcgggggg aagttaacgg aggtatcgcg
    12601 gatacaaacc gctggataat gacgcaattc actgttttct aatggatgcc gcttcgctgc
    12661 cagtgggtgg agtgggctga cgacaaaatc ccattccatt tgtccgaccg gttcgctaat
    12721 gatgccttca ctgcttggca ctgcatgagg agcaccgtag accagatcgg cacgcttact
    12781 gtacagtgca tcccagcagc cgttgtatac ctcaagatct accgtcagtg ttgttgatgt
    12841 gaattgccgt tcaaaatctc gaataaatgc caccagcaaa tcaccaggaa taatattgtt
    12901 taccgcaatt gtcagttcac gctcaatacc atcgtgaatc agcactgtgt tgcgctttaa
    12961 tgcttccagg tcgccaagaa tagttttgct atgttcgatg aagtaacttc ccgctggcga
    13021 taattcaata tagcgtccct tacgaatgaa cagttcaacg cccaggctct cttccgcttt
    13081 cttgatggtg taactgatgg cggaagggac tttatttaac tgctcggctg cggtggtaat
    13141 gctctggtgt ctggcgacca gatgaataac acgaagcgtt tcgtctgaga tgaacataag
    13201 gctccctggt cagtggtaaa gtggctgaat ctctccagat tcagccgtaa tcattgcctg
    13261 agtataccca ggaattagcg gtgttgactt gcccgactga gaatcgcata cgcgatacat
    13321 gccaccagaa tcgagtctac cgaagggatc gtcgtcaggg tgaataagcc ttttacggtc
    13381 ataaagccaa ccgctgcgcc tatgatccat gctgcgaatg tcagcacttt aatctgtgac
    13441 tcctgggcta acactgactc ttgatagcca ttacggcgat aaaggaaaaa gtcagcgata
    13501 taaattccgg caacgggtgg cgtggcgata cccaggaaca gcagaaacgg aataaaccac
    13561 gccataatat ccatactgcc tacgatggcg gaaaggatac ccagcgccac ggtaatctgc
    13621 catttgggaa agcgggtaag cagtgtggaa accaccagcg tgccctggaa catattgccc
    13681 gcgttaccgg tgacgcaggc gaaaatcaac aacagcgccg caggcagaac cgcaccgaat
    13741 accgccatcg cacccagtaa agatccctga ccactaatgg cgctcggcgt tgcacccgcc
    13801 cagtagagca gagggtaggc aaccagaaac gtcacgcctg cggcaatcag cgcatgtttg
    13861 cggttatgca caaaactacc gaaatcaggc agtgtggcaa ccagcacaat aatggtgcca
    13921 acaacggtgg aaacggcgac acctgtattc atctccacaa tcgccgctgg tactgccact
    13981 tcggtatggg ttgccacata gaggatgtaa cacagcacca gcgcgataac cggcacggca
    14041 acctgggcaa tttttcccag aacggtaaag ccaaatgccg tagaggcgac aaaaatgaca
    14101 cagccagccg ccactaacag tgccagtgga acttccagtc catgttgagc cagtaagtca
    14161 tgtaccgaat gaccaaacat attggcggta acggcaatcc agccgaacag acttatcgcc
    14221 atcaaaatgt taatggcgat ggcccctttt tcgccaaacg aatatttcac aatgccatac
    14281 gttggcaggc gggctttttc tcccacgcta atggtgatgg ctgaaagtaa ggtcagaatg
    14341 acgccgccta acaatacgac gctaattaaa ctgctgctgg aaagttgatt gccgaggctt
    14401 gaagatgaca acagcacagg tgtgacggca atacccagca aaatcatggc aatacgcaat
    14461 cctgatgccg tattcagatt ctcttctttt ttacgcatag tatgttccct ttataaatgc
    14521 gcgttacaca gtatgaaaat gcccggtgta tttgcgccag ctacccagtt ctgtaatttc
    14581 ccgttggcct tcggtcagcc agttttctgc cagtacgccc agcacttcgc taccatcggc
    14641 aagtttgatt ttgccaattg ccagcccagc gggttcgctc attaacagag cggcaaaact
    14701 ggccagcggt aattgccaaa tttctacgtc gacgtgtgtt cctccttcat tcacgcgaat
    14761 cattcccgga tggcgatcat taatactcca caaccggtaa tgggcgtcag ttcggtcttc
    14821 acggacaaaa ataccgcccg ctttttgcat atttgggttg agttccaggc cgcgcatcag
    14881 cgttccgtta acggctaaaa gcgttgtagt cattgtgttc tccagagaat taaagggtta
    14941 tccatgtacc gctctgatgc gccagggctg cttttatggt ttgcggtgaa gtaatcaggc
    15001 cgctggcctg tttgccttgt tgctggctgg cattgatgaa atcaacaatg gcttccactt
    15061 tgggttgcat actgccgaca ccaaactgac cttcccgcaa cagcgtttgc gcttcggcaa
    15121 cgctgatagc gtcgagccac tgttgttgtg gtgtaccaaa gttaatcgct actttttcta
    15181 caccggtggg gatcaccagc agatctgcgc ctaactgact ggcgagcagc gcggaggcca
    15241 gatctttatc gatcacggct tccacacctt tcagttgttg cccgtctcgc accaccggaa
    15301 taccgccgcc gccgcaggca ataaccagat atccctggcg caccaggtga gcgatggtgt
    15361 cgtgctcaat aatttccagt ggtgcaggag agggaactgt acgccgccaa ccacgcccgg
    15421 cgtcctccat cagcgtccag ccgagttgtt gttggcgttg ttgggttgtt gcttcatcga
    15481 gaaacgcgcc aattggttta ctggggctgg cgaaagcatc gtcatgtggg ctgactcgcg
    15541 tttgtgtcac cagggcaatt accggtttgt ttatgccacg gcgagccaat tcgttatgca
    15601 gcgctttctg gaacatgtag ccaattgcac cttgtgtatc acccacggcg taatcaagtg
    15661 gaaccggaga aacttcgtta ctggcgagtt cagagcggcg cagaataaag cccacctgcg
    15721 ggccattacc gtgggttagt accaggtccc atccggcttc aaccatatca acgatatgtt
    15781 gcacgctttc catcactgca acatattgat cgggaatact attgcgttgc tcgtcctgaa
    15841 tgagcgcatt gccgccaacc gcgacgacag ccagtggctt aaccatgatg gttctccttt
    15901 gcgacgatct ccgccagtgc acgtacagcg gcgacaaaac aggccatcgg cgcagtggtg
    15961 atcccggcac caatttgccc gacccccgct tgtttgtggg cgatacccgt attgatgacc
    16021 ggtaaaatgc cgcgatcggt cactttacgc gcatcaatcc ctgctgcggt gggggcaaaa
    16081 ttgagggcag gaagtgtaaa tgcggggtta ccaccgaggg tgattgcttg catcctacgg
    16141 ctgttattgg tggcatcggc aggggtgcct cccacaaatt tgacgatcgc tggagatgac
    16201 gccatcgcga agccacccac acctgcggtt tcagtaattg cgctatcacc caggtcagca
    16261 gcggcatcgt cgacgccaaa tccggggaag aacagacctt caacggcatt ggcaggagcc
    16321 tggaaccagc gatcgccagt accggaaagc cgaataccaa aattcacccc gttacgcgcc
    16381 atcaccgtaa ccattgaact gaacggtaca ttggcggcgg catccatcat tgctttacag
    16441 gcggccatcg aaagattgag gaagaagtga tcgttaccgg tgataaatgc cacgacgcgc
    16501 tggatatgtt cctgtggaag agagcaggcc agcaatgccg ggagcaggcg cttaatcagc
    16561 aaaccggtag cggcagcatt gcggttatga acttcatccc ccatatgcag cgcctgcgcc
    16621 attaatggct tgagttccag ctcgccgtgt tgtgcgattg ctgctttcat tgccggcgcc
    16681 agttcatcgc gcatccaggc cagacgattg agaacttcat cgttattggc gccaaaacgg
    16741 agaaccttac ccagaccttc attgaaattg ctaaaagtac gatggccgtt ggttttgttt
    16801 tctaccaccc ataacggcat cgacgggctg atgatgcctg ccattgggcc aaccgcgtga
    16861 taatgatgac aaggggcaag gtcgatttcg cctgcgttta tttggttttc agcgtcttgc
    16921 tggctggtgg cccaaccttc gtacagcatg gcaccaataa tcgccccttt taccggacca
    16981 cacatctctt cccaggcaat cggcgggcca gagtgcaaaa tacgtttttg ttcagccatt
    17041 gctggaatgg cctctttagc gagcatgaca tccatcagca cgggctgtgc ttcgagataa
    17101 cgtgtcatgg cgatttggtt ggcattttct accaggggat gacgtagcaa cgatgccaga
    17161 tccaatccag cgtcaatatc gccttgtgct ggtggctgcc aattgagtgc aattgcgctt
    17221 cccccggcgt gttgaatatt gtcagcaaaa ctgtgtagtc ccgcgttcac gaccttcaaa
    17281 ggctgattaa ataaggtatt catgcgttta gccccttttt ctgcaactga gtctgcgcaa
    17341 cggcgctggc ccatagcgcc gcctgtgcat tgctgctggc aatgatgatg cccgcatttt
    17401 gtagtgcgct tatctgctga ctgcgtattt gcggatcagc ttccgttccg caaacgtgcg
    17461 caatcagcaa cggtgcgtta ttcatgtcga tgtgtgaaag ttggtccagt aattcgctgg
    17521 caggcgtagt ggaagcgcca taaccaagta ccaggtcgaa taacacgacg gcagtgtgtg
    17581 agtcgttgag ttcgttgagc agacgttgat tgcgcagagt cgggtcgatc attggatgcg
    17641 gtttgccacg ggtgaaatca tcgtcgccca tatcgatcaa cgtgtgatct tcgctttgcc
    17701 agatatttgc cagagcacga ttacccgcaa ctggcgtatt ggaggctgcg ctgaatccct
    17761 tttgttggca gataagttgc gcttcgtagc agaaggttcc tccggcgaaa acaccgcgta
    17821 tcgcctggcg atgggctggc aggcgctggc aggcgttttg taacattgtg agatcgtcgc
    17881 aggaaatttc ggtctcgata gtcggaagtg gttgatcatt cagtaacgcc acggcgatat
    17941 ttgcggcgct ggcaagtgtg gtggccgcgg tgatattggg acgtgccaga tcatgcggat
    18001 tcgcaccgag gaaattcacc accacgggtt ttccacaagc ctcggcacgc tccagaatag
    18061 tccgggcaac gatcggtgaa ggcggttttg aaatcagcac aattacgcga gtttcatcgt
    18121 cttgcgccag cgcatctaaa gcgaagagca tcgatattcc accaatctct tcactgagat
    18181 catgtccgcc tgttcccaac gcctgagaaa tacccgctcc gagctggtcg attcggcaag
    18241 tcacttcctg tagcccggtg cctgacgcgc caataacacc aattgctccg cgtttcacca
    18301 cattagcgaa gcctaacgga atgccgttaa caatggcggt tccacagtca ggtcccatta
    18361 cgatgcgctg acgttctcgg gcaagcgttt tgatggattt ttcctgcacg atactgacgt
    18421 tatcgctgaa catcatcacg ttcattccga ggtttaacgc tttaattgct tcggcggcgg
    18481 cgtagtctcc cggtacagaa atgagtgcca ggttggcttc gggttcggct tccagtgcca
    18541 tttccaggct gaccttttcc ggtgatttaa tgccactatc cgtttcatca tcgggtttgc
    18601 tggtcagacg ttgctgggcc agtaccagtg cttcattgca aatatcttct tcacccatga
    18661 ctgcaatcac taaatcattg ggactggcgt tgatttcgtt acccagaccc gcatcgcgca
    18721 attgttcgag attattgggc gttcccatga cgaccgaggc ctgttgaata ccgggcaact
    18781 tgctgatttg cgcggaaatc tgcatcagag aaactgagtc ctgataaaga ttactgaaaa
    18841 ctttatattt aactttcatg ctgttacgcc tgtttcattc cgcccgatag caaaattcgg
    18901 gcaaagcatc cccgtgtagc cccataaacg ggctacggga gaaaaggttg aaatcaattc
    18961 atggtgttca gggttcgaaa gccatgcagc attcctgcaa ggcaatctac tcctgacgtt
    19021 gcgccaaatt gcatgacctg ttctgcacag gatgcgattg tcattgccga tgcgctccca
    19081 accagttgtg cgagtaactg gcaaatcggt tctgaaaaat gtccctgaag cgcacgctcc
    19141 agataatgac gactgatatc cgtggtgttg tgcgcatgtt gatcaattgc ctgctgcaac
    19201 cggtattgat gatcggcaag tggtgcaggg agttgccata accagagcgc tgccagatag
    19261 cccaacaaat agtcatctcc atcgggcgtt aagcctttac caaagccgat cagttgtggc
    19321 acctggcgtt ccagcgcatc tggatgctcc ataaccgcta atccctggtg cgtgagacta
    19381 tccggcaggt gcaatgtgct ttgaacgtta tgttccagac aaaataaaag ccgttgcgat
    19441 gccatcgttg ggtagtgctg gaaaaccaaa gggaatgcct caagtgtcag cattcccccc
    19501 acaaatggcg actgccagag cgttgcgtta tcgagcgcca cacaaaatcg aggtgtccgc
    19561 agtaagcctg gggttaactg aatgggatct gaatgggcaa tctctcttcg ccagtcccaa
    19621 cattcaggta gccagactct ggcagcatca gggagattca gatacgtttg cgcacacaac
    19681 agagtgatga atgttcccgc gtctgaatac agatttaccg catgtttaaa agcgctatgc
    19741 agacgtaaat cttgcgtttc attctgcggg aaaagatatc ccaacgacga tgcgcaaagc
    19801 gtaacctgta ccatcggcgt tagccttcca gacctgcaat aatggctttg gaatcagtaa
    19861 cccaaccgaa aatggcacct tgtgcttgat catctcgagg gcgtatttct ggaattccgg
    19921 gaaataggag ccgacacaat cttccgggat aatgcattca tagccacgat catttgcttc
    19981 gcgcacggtg gtggttacgc aaacttcggt tgtaacacca caaactatca gcgtcttgat
    20041 accgtggttt tgcaaaatca gatgtaagtc ggtttgataa aacgcgcctt tgccaggctt
    20101 atcaatcacc ggttcgcctg ccaccgggta cagttcagga atgatgtcat gacctgcttc
    20161 acctctgacc agaatgcgtc ccatcggtcc tggttcacca ataaaggttt ttccgccgcg
    20221 cgtcagtttg gctggcgggc agtcgcttaa gtctgcacga tggccttcac gagtatgaat
    20281 aaccataatt cctttttgac gtgcggctgc cagcacctct gtacatggtg cgatagccgt
    20341 gcgtaccagt gaaacatcat tgcccagtgc ctcaccgaag ccccctgctt cgacaaagtc
    20401 acgctgcatg tcgatcatga ccagtgcagt tgtgcgcgga tcaaaaggta attcaaaggg
    20461 ttgagcctga aaaatggatt gcgtcatggt aaatctcctg tctgatgttt aaacgatcca
    20521 gttgttctga gaacgagtat tcaggagaaa agcggcacaa aacaggaaaa aagtttgccg
    20581 cacagcaacg aaatttttgc attcagcagg tggtgtgatg acgaatgtga agggagtcaa
    20641 agagtggcat acagcaacaa attttttgat gctgtatggt tttgagatta aaaaaatcgt
    20701 tgaaattgtg tgagcttaat catcagattt agcgactatg gcgttacttt ttaacaccat
    20761 actgtgtaaa ccattccaac attcgctgcc agccgtcttt cgcagactcg gcatgatagc
    20821 tcgggcgata atcggcgttg aatgcatgcc cggcatccgg gtagacgata atctctgctt
    20881 tcgcgtttgc tgcccgcagc gcctggcgca tcgtttccac gctctcttgc ggaatgctgt
    20941 tatcctggcc gccatataag ccgagaaccg gcgcgttaag atcggttgcg atatcaacag
    21001 gttgtttcgg tgaattcagc gacttatcgc ctgtcagctt gccgtaccac gccactgcgg
    21061 cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc cagcagaatc
    21121 cggtgatcat taaacgatga acatcgccgc cgttgcgtga cgcccaactg gcgacatgat
    21181 cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta agcagcgtag
    21241 ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt gcgatagcca
    21301 gatacccctc cagcgccaga cggcgacaaa tatcgcggat atgttcatgc acgccaaaaa
    21361 tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt ctggcatggt
    21421 aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca atcgcgtcgt
    21481 ccggagtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat ccagattgtt
    21541 gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg ttaactatag
    21601 tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt cctgcagaat
    21661 gaagagtgat ttatgtgatt tgcatcactt ttggcgggta aatttatgca acgcattttc
    21721 gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt ctttttgctt
    21781 cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg tttttcatct
    21841 cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg gcgacccgga
    21901 tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat ctcaccgcga
    21961 attcaccacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct ctaccggtat
    22021 cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca ttcgcacctt
    22081 cctgcgtatc ggcacaacgg gcgctattca gccgcatatt aatgtgggtg atgtcctggt
    22141 taccacggcg tctgttcgtc tggatggtgc gagcctgcac ttcgcaccgc tggaattccc
    22201 ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat ccattggcgc
    22261 gacgactcac gttggcgtga cagcttcttc tgataccttc taccctggtc aggaacgtta
    22321 cgatacttac tctggtcgcg tagtccgtca ctttaaaggt tctatggaag agtggcaggc
    22381 gatgggcgta atgaactatg aaatggaatc tgcaaccctg ctgaccatgt gtgcaagtca
    22441 gggcctgcgt gccggtatgg tagcgggggt tatcgttaac cgcacccagc aagagatccc
    22501 gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg tggaagcggc
    22561 gcgtcgtctg ctgaaataaa aaacgctccc caacttcggg gagcgatatc agttattgac
    22621 gccgtaaacg cattattttg cggattattg gcgtcagtag cgtaaaggct gccagagtca
    22681 gcaatgccag agtgatcggt cgggtataca gaaagtcgag tgacccacca gaaagcgaca
    22741 gcgcacggcg aaaattactt tccgccattg gccccataat caatgccagc actacgggtg
    22801 atgccggata tcctcctttc tgcatgaaat agcccaccac gcctgcaatc agcataatgc
    22861 caacatcaaa aaggctattg ttcaacgcat aagtcccgac cacacaaagt gcgaggatca
    22921 tcggtgtgag aatattgccg ggaattttca ccacattacc gatgactttc aaagagagca
    22981 gtccaagaac tagtagcatg aagtaacaga agatcatgcc gacaaacagt gtataaacca
    23041 tatcgccgtg gtcggtaaac atcagcgggc ctggttgcat tccctgtaac gttaacgcgc
    23101 ccagcatgat ggcggttacc gcatcgccag ggatccccag cgtcagcata ggtaacaatg
    23161 cgccaccggt gcagccattc gcgcctgcct cacaggaggc cacagcttta atttcaccct
    23221 taccaaagtt ttctggtgtc ttactgaaac gacgagtttc gttataggca acaaaagctg
    23281 caatatccgc ccccgcaccg gggatcatgc cgataaaaac ccctaatcct gatgagcgca
    23341 aaatggttaa tgcaatgcgg cgtagtgttt gccagggcag taacagactg ccgatagcca
    23401 ccttcgcccc ctgacgaatt tgctggtttt gctccataga gcggaatgct tctgaagcag
    23461 caaataaacc aatcatcacc gggataaatg gcacattcat cagctccaca aatccacctg
    23521 taaaccgtgg aaaaccgccc attggatcaa ggcctatcgt tgcgatcagc aatccgccaa
    23581 ccccggcaat cagcccttta atcagcgact caccggaaat actggcaata atgctcagtc
    23641 cgaaggttgc cagggcgaag gactccgagg cactaaattt cagcgcccaa ctggcaagta
    23701 ccggagagag aaacatcagc acgataatgc tgagcgttcc gccactaaag gaagagaacg
    23761 tggcggcact caatgccagc cctgcttttc cctgtttcgt cagctcataa ccatcaatag
    23821 cggtggctgc ggcggcaggc gttccgggaa ttttgagcaa aattgcggtg atagaaccac
    23881 cgtagacgcc accaaaaaag acgccgcaaa tcatcaacaa gccagaaaca ggctccatgc
    23941 cgtaggtgaa aggcagcaaa atcgccacac ccatggtcgc tgtcagacca ggcaaagcgc
    24001 caatcacaat acccagcatc accccaaaag tggcaaacag cagcgcctga ggattattga
    24061 gtaatgtcga aaaaccctga gttaaaagtt cactttcaaa cattgctggc ccctgttatt
    24121 cgaaaaatgt gccgacaggc aatggcacct gtaacgcttc gacaaacagc aaccagagga
    24181 ttgccgttaa cagcagcgca atacccggtg tgagtaccca tcgcctgtag cccatcaacg
    24241 tcataatgac gatgaggaaa acaggagtag taatgagata accgcagtag ctcatcgcca
    24301 caatataaaa tgcggttgcc gcaattcctg ttgccgtttt cctgaatgcc ggtttttcct
    24361 gtgcgttggc gcaagaggct ggttttgcct gcaaaagatc gcgtatcacc agcaagacag
    24421 cgagcacaat cagggcacag gcatagaagg tagggaaggc acttgcaccg ggatccgttt
    24481 caatggcagt tgctggaaaa tcagcggcat taatcatcac cgcgccagca accacacaaa
    24541 gtaacagcgc caccaccatc gttgatgaaa ggcgcttgcg gtgcggtacg gcgagttgag
    24601 caacagactg ttttgtgttc atcaaccgta cctcaggttt attttttcag gcccaggcga
    24661 gtcagcaact cgtcaaagta cttttcctgt tcgctgattt gggtctggaa gctggcagcg
    24721 tcaagccatg catagttgag attcaacttt tgcagtgcat cctggaatgc aggctcttca
    24781 gccgtctctt ttgctgcctt tgccagaaca tccaccacat cctgcggcgt tttttgcgac
    24841 acaatcaggc cgcgccaggt gccgatggag agatcaacgc ctttctcttt taacgtcggg
    24901 acgtcaggca tggtttccat tcgctcatcc gccatcacta ccagtgtctt cagcttgccg
    24961 cctttcacat gattgataac ttcacctggg cttacggaaa ccgcttcaat atgcccgcct
    25021 aacaggcctg taatggcagg ggctgcgccg tcatacggga catgagaaaa cttcgcaccc
    25081 gttttgtctt ccagtgcagc tgccgccaga tgccagatag ctccggtgcc tgagttacca
    25141 atgcgtactt ttccgggatt cgctttggcg taagtcataa attcttcata gctattccac
    25201 ggcgcatcag aacggacggt gatagcagcc ggatccgcgt ttagacgggc aatgggttta
    25261 aaatcgctgg ttttaaaacg caccattccg aggctgggaa gggtggtcag ttcaaccgtg
    25321 cctaaaccaa ttttgtaacc gccagggcgg gcagcggcga tttcactcag gccgatagca
    25381 ccgcctccgc caggtttatt gataaccccg atgctgacgg gtaaatggtt tttggcggca
    25441 tcagcaaagg cacgggcaac cagatccgta ccgcctccgg cagcgtaggg aacgactaac
    25501 tcgatctgtt tggttgggta ggaggctgcc tgtacaggcg caatgctgag ggcagcgcct
    25561 gcaagaagta ccattgcagc catttttttc ggtaaggtaa gatgttttat cattgctttt
    25621 atatccttat ttatcagtta gttgctattt aaattgttcc atgcgttcat aaacagtact
    25681 gcattttcat agacttgctc tggcgtcatg ccggggtgat atagcgcaga accgaggcca
    25741 aagccgtttg cgccgtggct cagataacgc gccatggttt ccggtgtgat accgccaacc
    25801 ggtaacatcg gaacggattg tggaataacc gcacgccagg ctttggtaac ttctggcgta
    25861 atttgttcag ccgggaacag cttgatggca ttagcaccat gttcaatggc agcaaaggct
    25921 tcggtgggcg tggcgacgcc aggcgcacaa tacataccca gcgcacgggc gcgacggatc
    25981 accgcagtat cgcaatgcgg catcacgata atctggcccc cagcctcttt aaccgcaacc
    26041 acctgctcta ccgaaagcac agttccggct ccgaccaggc agtccttagg cagggcgtcg
    26101 cgcagtaaac tgatactttg caacgcctcc ggcgagttca taggaatctc aatcaggcgg
    26161 aatccggcgg cgtacagttt ttcaccgatg gcgacaatct catccggttt cacaccgcgt
    26221 aaaatggcga ctaaaccggt tttttgcaaa agcgtattaa atggcatggg tacattccct
    26281 ggcgttggtg gacagtaacc cggcagcgtg ggcgattcgc cataatccct gttcagtggc
    26341 attgggaatg gcgtgtatct gtctgtcggg aaaaacgtgg ttgaatgctt gtgaatagcg
    26401 gctgttcaga tttgcgctac cgattaaggc gatcggtgtt gtggcgggta aatcacgtag
    26461 caaactggat gccagaccac ataattcatg gcctatcagt aatccggaaa ggtaatcccc
    26521 ctgcgaggac gacgtaagat tactggtcag caggcgcgaa cgggtcgaaa acagcgtcga
    26581 taataatccg gtctgatgat gctcttgtgc tgctgtcagc ccgtgggtga aggcatccca
    26641 gtgcggttca tctccaggat gcatggtggc accgagaatc gaatggcggg ataaaacatc
    26701 aaataactcg cccgtcataa aggttcggaa atcggtaatg gtgttgtttt caactactgc
    26761 ccatttcgca tgtgtgccgg gtaagcctat cagcactggc ataccctgat ggatcgcgtt
    26821 ttgcagggcc ggttccatcg agatagcacc gaagatttgt gtctcttcgc cacgcatcac
    26881 ttcgggcatt tcaccttctt taatcacgcc aggaatgatg tgcagcattg tctgctgctg
    26941 gtggataaca ggcgtcaact gcttcgccag accctcgaga gtgaccgggc aaggtaaata
    27001 agctgctggt tgccaccctt gcgcactgcc gagcatgccg caagccagta ccgggcatgg
    27061 ggtttgtgcc agccagtcgc cgcagacacg taaaaacgta tcgtggaaag cgtcctcacg
    27121 tgttgctgcc tgggagggta atttcatgat cccccagggg aattcacgtt tttcttgtgg
    27181 atttggtgat tcgccaaaac gccatgcccg aagcgatgat gttccccagt ccagggcaat
    27241 aaatgagggt gctcccggca ttagtcgttt cctttaatca tggtttattt atctttgcgg
    27301 acgtgcttac cccagcccag ttcagcggat atctcttctg cacaggattt gacgtaaggt
    27361 gccagttctt ccagacgcgc cagcgacata tacgtggtgg tactggcgac agaaatagcc
    27421 gccacaattt catcccgggc gttgtaaacc ggcgctgcaa cgcaacggat agtcggctcg
    27481 ttctcttcca gatcaaaagc aaaaccactg gcggcataac gacgcatgtt ctgaataaat
    27541 gcgcccagtt ttgtttcatc accacaggtc ttaaacagcg tgcgccactc ttcttctgtt
    27601 cggttgagga tcaatgcctt gccgatgccg gtgattgcca gtggcatgcg atggcctggg
    27661 cgtgagcgca tttccagccc gcgctgactg ttgatcttct ccagatacag cacttcgtcg
    27721 ccttcaacga tccccagatg cacggtgtcc agggtgtaac gggcaaggcg ttgcaaaacg
    27781 ggctgggcaa tttcgtacag cgaggtgctt tccagtgctc tggcaccgaa ttcgatcagt
    27841 ttggcaccca gttggtagcc ctgcgttggg acatggcgca ggtagcgttg ttccaccaga
    27901 acggtgacta aacgatgggt tgtgcttcgg gtcgtgccgg tgaactcgcc gatacttttt
    27961 aggtcatggc agccgttata aacagcatta agaaccgcca gtccacgtaa taacgtttgt
    28021 gcgcctgcgg tggttgaaga ggtttctgcc atacgtttct cctgatgata ttgctgaagt
    28081 gttccacata atgacgtggg gaaaaggtga tatatcccac aatataaact ccatctcata
    28141 ttgtgggata aaataatgaa aattaacgat cgttttgcgt agcgcctcgc aggaaacttc
    28201 tttcaaactg gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag
    28261 gaggcgttgt ggatttctca atcatggttt acgcagttat tgcattggtg ggtgtggcaa
    28321 ttggctggct gtttgccagt tatcaacatg cacagcaaaa agccgagcaa ttcgctgaac
    28381 gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa agcgagcact
    28441 ggcgcgctga gtgcgagtta ctcaataacg aagtgcgcag cctgcaaagt attaacacct
    28501 cgctggaggc cgatctgcgt gaagtaacca cgcggatgga agccgcacag caacatgctg
    28561 acgataaaat tcgccagatg attaacagcg agcagcgcct cagtgagcag tttgaaaacc
    28621 tcgccaaccg tatttttgag cacagcaatc gccgggttga tgagcaaaac cgtcagagtc
    28681 tgaacagcct gttgtcgccg ctacgtgaac aactggacgg tttccgccgt caggttcagg
    28741 acagctttgg taaagaagca caagaacgcc ataccctgac ccacgaaatt cgcaacctcc
    28801 agcagctcaa cgcgcaaatg gcccaggaag cgatcaacct gacgcgcgcg ctgaaaggcg
    28861 acaataaaac ccagggcaac tggggcgagg tagtattgac gcgggtgctg gaggcttccg
    28921 gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc
    28981 ggatgcagcc ggatgtcatc gtgcggttgc cgcagggaaa agatgtggtg atcgacgcca
    29041 aaatgacgct ggtcgcctat gaacgctatt tcaacgccga agatgactac acccgcgaaa
    29101 gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag
    29161 attatcaaca gctgccgggg ctgcgaaccc tggattacgt gctgatgttt attcccgttg
    29221 aacccgcttt tttactggcg cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa
    29281 acaatatcat gctggttagc ccgactacgc tgctggtggc gttgcgcact atcgccaacc
    29341 tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc
    29401 tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa agtctcgaca
    29461 aagcgcagga taactaccgc caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc
    29521 tggcgcaggc agaagcgttt cgcggtttag gggtagaaat taaacgcgag attaatccgg
    29581 atttggctga acaggcggtg agccaggatg aagagtatcg actgcggtcg gttccggagc
    29641 agccgaatga tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca
    29701 ttgggggtag ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct
    29761 ggaacaattt tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac
    29821 tttggttttc agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat
    29881 tccgtggcat caaaatacga tgtcatgaat gatttgatgt cattcggtat tcatcgtttg
    29941 tggaagcgat tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg
    30001 gctggtggca ccggcgacct gacagcgaaa ttctctcgcc tggtcggaga aactggcaaa
    30061 gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat
    30121 atcggtgtga ttggcaacgt tgagtatgtt caagcgaacg ctgaggcgct gccgttcccg
    30181 gataacacct ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat
    30241 aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag
    30301 ttctcgaagc caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg
    30361 ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa
    30421 tccatccgta tgcatcccga tcaggatacc ctgaaagcca tgatgcagga tgccggattc
    30481 gaaagtgtcg actactacaa tctgacggca ggggttgtgg cgttgcatcg tggttataag
    30541 ttctgacagg agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct
    30601 gctcaacacc ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgttggg
    30661 caaagtgctg cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga
    30721 acgccaggtt gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgccta
    30781 tgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt gccgcactga ttcgcagtgg
    30841 tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct
    30901 ggcagagttc gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg
    30961 aatcagcaaa gccatgcgcg gaggcgcaaa gttcctgcat cacggcatta agcgccagca
    31021 acgttatgtg gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt
    31081 ggcctggttt gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg
    31141 gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt
    31201 cgcacttttt taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg
    31261 ctacggctat ggcgatactc attattctgg atgccaaatc ggcataaaga caaaccttta
    31321 ggtgagcgac tacgactggc cctgcaagaa ctggggccgg tatggatcaa gttcgggcag
    31381 atgttatcaa cccgccgcga tctttttccg ccgcatattg ccgatcagct ggcgttattg
    31441 caggacaaag tcgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg
    31501 ggcggcttgc cggtagaagc gtggtttgac gattttgaaa tcaagccgct ggcttctgct
    31561 tctatcgccc aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa
    31621 gtcatccgcc cggatatttt gccggttatt aaagcggatc tgaaacttat ctaccggctg
    31681 gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg
    31741 cgcgagtacg aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc
    31801 attcagcttc ggcgcaattt tgaagacagc ccgatgctct acatcccgga agtttaccct
    31861 gactattgta gtgaagggat gatggtgatg gagcgcattt acggcattcc ggtgtctgat
    31921 gttgcggcgc tggagaaaaa cggcactaac atgaaattgc tggcggaacg cggcgtgcag
    31981 gtgttcttca ctcaggtctt tcgcgacagc tttttccatg ccgatatgca ccctggcaac
    32041 atcttcgtaa gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt
    32101 gttggctcgc taaacaaaga agataaacgc tatctggcag aaaactttat cgccttcttt
    32161 aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt caggctgggt gccaccagat
    32221 accaacgttg aagagttcga atttgccatt cgtacggtct gtgaacctat ctttgagaaa
    32281 ccgctggccg aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc
    32341 ttcaatatgg aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctatgtcgaa
    32401 ggggtaggac gccagcttta tccgcagctc gatttatgga aaacagcgaa gcctttcctg
    32461 gagtcgtgga ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa
    32521 gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaat tggtttacga cagtttgcgc
    32581 cagggcaagt atttacagca cagtgttgat aagattgccc gcgagcttca gtcaaatcat
    32641 gtacgtcagg gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc
    32701 acattcttgt tggtcagccg acctgaatgg gggctgatgc ccggctggtt aatggcaggt
    32761 ggtctgatcg cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg
    32821 cgggccgtgt aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt
    32881 atgggtggta tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt
    32941 ggcaccaaaa agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa
    33001 aaagcaatga gcgatgatga accaaagcag gataaaacca gtcaggatgc tgattttact
    33061 gcgaaaacta tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac
    33121 gcgaagcgcc acgataaaga gcaggtgtaa tccgtgtttg atatcggttt tagcgaactg
    33181 ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta
    33241 aaaacggtag cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa
    33301 ctgacccagg agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg
    33361 agcctcacta acctgacgcc cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg
    33421 gagtcgatga agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac
    33481 accatccata acccggtggt gaaagataat gaagctgcgc atgagggcgt aacgcctgcc
    33541 gctgcacaaa cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg
    33601 gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat
    33661 aaaccgtaaa catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc
    33721 gtaagcgtct gctgaactgc attatcgcgg tgatcgtgat attcctgtgt ctggtctatt
    33781 tcgccaatga catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt
    33841 caacgatgat cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta
    33901 tggtgtcgct gattctgtca gcgccggtga ttctctatca ggtgtgggca tttatcgccc
    33961 cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc
    34021 tgttttatat cggcatggca ttcgcctact ttgtggtctt tccgctggca tttggcttcc
    34081 ttgccaatac cgcgccggaa ggggtgcagg tatccaccga catcgccagc tatttaagct
    34141 tcgttatggc gctgtttatg gcgtttggtg tctcctttga agtgccggta gcaattgtgc
    34201 tgctgtgctg gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc
    34261 tggttggtgc attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc
    34321 tgttggcgat cccgatgtac tgtctgtttg aaatcggtgt cttcttctca cgcttttacg
    34381 ttggtaaagg gcgaaatcgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg
    34441 aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat
    34501 cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg tagcgtgcgc
    34561 ttttgacgcg ggagttaatg ggctactcat caccggcact aacctgcgtg aaagccagca
    34621 ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca
    34681 cgacagcagc cagtggcaag ctgcgactga agaagcgatt attgagctgg ccgcgcagcc
    34741 agaagtggtg gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga
    34801 agagcaggaa cgcgcttttg ttgcccagct acgcattgcc gcagatttaa acatgccggt
    34861 atttatgcac tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc cgtggctgga
    34921 taaactgcct ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg
    34981 cgtggcgcat ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcggact
    35041 ggagctgcgg gaacttttgc cgttgattcc ggcggaaaaa ttactgatcg aaactgatgc
    35101 gccgtatctg ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc
    35161 ccatctgccc catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct
    35221 ggctgccacc acggatgcta atgtcaaaac actgtttggg attgcgtttt agagtttgcg
    35281 gaactcggta ttcttcacac tgtgcttaat ctctttatta ataagattaa gcaatagcat
    35341 ggagcgagcc tcaccatcgg gttcggtgaa aatggcctga aagccttcga acgcgccttc
    35401 ggtaataatc accttatctc ccggataagg ggttgccgga tcgacaatgt ctttcggttt
    35461 atataccgat agctgatgaa tcaccgccga tgggactatc gctggcgacg cgccaaagcg
    35521 cacgaagtga ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttctggatc
    35581 aaattcgaca aacaggtagt tgggaaacaa tggctcactg actgcagtac gttttccacg
    35641 cacgattttt tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg
    35701 ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat
    35761 gactcttatc cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat
    35821 attccccggt ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg
    35881 caaagatgaa cgccgtataa tgggcgcaga ttaagaggct acaatggacg ccatgaaata
    35941 taacgattta cgcgacttct tgacgctgct tgaacagcag ggtgagctaa aacgtatcac
    36001 gctcccggtg gatccgcatc tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg
    36061 tgggcctgcg ctgttgttcg aaaaccctaa aggctactca atgccggtgc tgtgcaacct
    36121 gttcggtacg ccaaagcgcg tggcgatggg catggggcag gaagatgttt cggcgctgcg
    36181 tgaagttggt aaattattgg cgtttctgaa agagccggag ccgccaaaag gtttccgcga
    36241 cctgtttgat aaactgccgc agtttaagca agtattgaac atgccgacaa agcggctgcg
    36301 tggtgcgccc tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca atcgcattcc
    36361 cattatgacc tgctggccgg aagatgccgc gccgctgatt acctgggggc tgacagtgac
    36421 gcgcggccca cataaagagc ggcagaatct gggcatttat cgccagcagc tgattggtaa
    36481 aaacaaactg attatgcgct ggctgtcgca tcgcggcggc gcgctggatt atcaggagtg
    36541 gtgtgcggcg catccgggcg aacgtttccc ggtttctgtg gcgctgggtg ccgatcccgc
    36601 cacgattctc ggtgcagtca ctcccgttcc ggatacgctt tcagagtatg cgtttgccgg
    36661 attgctacgt ggcaccaaga ccgaagtggt gaagtgtatc tccaatgatc ttgaagtgcc
    36721 cgccagtgcg gagattgtgc tggaagggta tatcgaacaa ggcgaaactg cgccggaagg
    36781 gccgtatggc gaccacaccg gttactataa tgaagtcgat agtttcccgg tatttaccgt
    36841 gacgcatatt acccagcgtg aagatgcgat ttaccattcc acctataccg ggcgtccgcc
    36901 agatgagccc gcggtgctgg gtgtcgcact gaacgaagtg tttgtgccga ttctgcaaaa
    36961 acagttcccg gaaattgtcg atttttacct gccgccggaa ggctgctctt atcgcctggc
    37021 ggtagtgaca atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg
    37081 gtcgttctta cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgttaa
    37141 cgcacgcgac tggaacgatg tgatttgggc gattaccacc cgtatggacc cggcgcggga
    37201 tactgttctg gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtctccgg
    37261 gctgggttca aaaatggggc tggatgccac gaataaatgg ctgggggaaa cccagcgtga
    37321 atggggacgt cccatcaaaa aagatccaga tgttgtcgcg catattgacg ccatctggga
    37381 tgaactggct atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt
    37441 atcgatccga cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc
    37501 tatcacggat accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc
    37561 tggtcagtat ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcttc
    37621 gacgccggat gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta
    37681 cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg acattcccca
    37741 cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac
    37801 cgggttctct tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga
    37861 tatcaccatt tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga
    37921 ggcgctttcg ttgaagcatc ctggtctgca agtggtgccg gtggttgaac aaccggaagc
    37981 gggctggcgt gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc acggtacgct
    38041 ggcagagcat gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgcgatct
    38101 gttttgcagt gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat
    38161 ctgagatata aaaaaacccg cccctgacag gcgggaagaa cggcaactaa actgttattc
    38221 agtggcattt agatctatga cgtatctggc aaaccatgcc cgatgcgacg ctgtcgcgtc
    38281 ttatcgtgcc tacaaatagt ccgaaccgta ggccggataa ggcgtttacg ccgcatccgg
    38341 caattggtgc atgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgcc
    38401 ggatcggtag accggataag gcgttcacgc cgcatccggc aagtggttaa acccgctcaa
    38461 acaccgtcgc aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt
    38521 ctttgcgttc catcagattc agcagcgtgg tgctgatacg cgcaccggaa caacccagcg
    38581 gatgacccag cgcgatcgcg ccaccgttga ggttgatctt ctcgtcaatc tgctcaatta
    38641 gtcccagatc tttaatacat ggcaggatct gcgcggcaaa ggcttcgttc atttcaaaca
    38701 cgccgatatc gctggcagaa agccccgctt ttttcagcgc cagtttcgag gccggaaccg
    38761 ggccgtaacc cataatcgat gggtcacaac caacgaccgc catcgaacgc acacgagcgc
    38821 gcggcttaag acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc
    38881 catcggaaag tgcagaagat gtgcccgccg ttaccatacc gtttactgga tcaaacgccg
    38941 gacgcagcgt ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa
    39001 actgcttcag gacgccgtcg gcatcgtgac caccggtcgg gatgatttca tttttaaatg
    39061 cggccgactg cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca
    39121 tttcacggct gataccgtgc atacgcgcca gcatttctgc cgttaagccc atcatgcccg
    39181 ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca
    39241 catggcccat atgctccacg ccgccaacca gacatgcctg cgcatcgcca gtcatgatca
    39301 ttcgtgctgc gtcatgcagt gcctgcatgg atgaaccaca caagcgatta acggtaaccg
    39361 ccgggacaga gtgtggtact tctgccagca gcgccgcgtt acgggcgata ttaaaaccct
    39421 gctccagcgt ctgctgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg
    39481 ccgggttacg cgccagcagg ctacgcatta aatgagcgga gagaccttct gcacgcacgt
    39541 tacgaaaagc accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa
    39601 cctgttccat tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa
    39661 ccggaggata gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcat
    39721 acagcgggcc gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg
    39781 tgtccagcca gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg
    39841 ccatatccgc ttccgccgga gtggcgataa tgccttcctc cagacagcgc accacttcgt
    39901 tgaccatcgg gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggct
    39961 ggctcacttc tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc ggcttacctt
    40021 tgctgtcttc tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat
    40081 caaacagcgc gtcgatggca tcgcggtaat ctttctgcat ccgctgcggg aagcctgctg
    40141 ccatgacagc ctgagcgtga tgcgcggtat caatgcccac aacgtccagc agatatgccg
    40201 ggcccatcgg ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc
    40261 cgtcgcgcag cagctggctg aaaccggcga aatacgggaa cagcacgcgg ttaacaaaga
    40321 agccggggca gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc caggcgacaa
    40381 ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcattc
    40441 ggtggaccgg gttaaagaag tgcatcccgc agaagttttc cgggcgttcc agcgcgttgg
    40501 ccagttcgct gataggaatg gttgaagtgt tagacgccag cacggtatcc tggcgtactt
    40561 tttgttcggt ttctgccagt acggcttttt tcactttcgg gttttcaaca accgcttcta
    40621 ccacaatatc cacgcggtca aatccggcgt agtcgagcgt tgggtggatt gtggagatca
    40681 cgccagccag tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg
    40741 cggcttcggt catgccgagg gttaacgact tgtcgttgat atctttcatg acaaccggca
    40801 cgcctttcca cgcagactgg taagcgatgc cgccgcccat aatgcctgca cccagcaccg
    40861 cggcctgttt cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt
    40921 gatcgttaag gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc agcgggacaa
    40981 aacttttgtt ttccaggttt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg
    41041 tttttactgc ggtgatgggg gccggataat gtttccccgc tgtttgtgcg accatccctt
    41101 tagcgatggt gaagctcatg gtggcttcaa tcttgctcag ttttagtggt tccagcttcg
    41161 gctgacgttt tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa accgcctttg
    41221 cgccttcaac cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct
    41281 gatccgcgcc gacatcttta ccggcggcaa tgatttccag cgcactgtca gcgcccagca
    41341 tacgtggcat acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca
    41401 gaccgatgcg cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc
    41461 caccgcccag cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc
    41521 gattaaacac gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga
    41581 acagggacaa aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca
    41641 gcagcagccc ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg
    41701 cgacggtcgc agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa
    41761 tgccatcttc cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct
    41821 gaatccgcaa ggtgatctgg tcgtaccaga tgagtcgaag tgtgtatttt gtgttaaaag
    41881 tatgcaaaca aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct
    41941 ggatgaacga tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat
    42001 ggaatcactg gcctcgctct ataaaaatca tatagctacc ttacaagaac ggactcgcga
    42061 tgcgctggcg cgcttcaagc tggatgcgtt acttattcac tccggcgagc tgttcaatgt
    42121 ttttctcgac gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc
    42181 ggtaactcag gtgccaaact gctggttgct ggtggatggc gtgaacaagc cgaaactgtg
    42241 gttttatctg ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac
    42301 tgaagatgtg gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc
    42361 tgcgcgcggc aatatcggtt atatcggtcc ggtgccggag cgtgcgctgc aactggatat
    42421 tgaggccagc aacatcaacc cgaaaggggt tatcgactac ctgcattact atcgctcctt
    42481 caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca
    42541 tcgtgcggca gaagaagcgt tccgttctgg catgagcgag ttcgatatca acattgccta
    42601 tctgaccgcg accggtcatc gtgataccga cgtaccttac agcaacattg tggcgcttaa
    42661 cgaacacgct gcggtgctgc attacaccaa actggatcat caggcgccgg aagagatgcg
    42721 cagcttcctg ctggatgccg gggccgaata taacggctat gcggctgacc tgacccgtac
    42781 ctggtcggca aaaagcgata acgactacgc gcagctggtg aaggacgtta atgatgaaca
    42841 actggcgctg atcgccacca tgaaagcagg cgtcagctat gtggattacc acatccagtt
    42901 ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga
    42961 agcgatggtc gaaaacgatc tcaccgggcc gtttatgccg cacggtatcg gccatccgct
    43021 gggcctgcag gtgcatgacg tcgccggttt tatgcaggat gatagcggta cgcacctcgc
    43081 ggcaccggca aaatatccgt acctgcgctg cacccgtatt ctccagccgg gcatggtgtt
    43141 aaccatcgaa ccgggtatct acttcattga atcgctactg gcaccgtggc gtgaagggca
    43201 gttcagcaag cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg
    43261 tatcgaagac aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa
    43321 actggcgtga tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc
    43381 aaaaagagcc gcttcataac gatgttggcg cataccgatg gcgttgaggc ggcgaaagcg
    43441 tttgttgaat cggtgcgggc agaacacccc gatgcccggc accattgcgt ggcgtgggtc
    43501 gcgggtgcgc cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcggga
    43561 acggcaggta aaccgatgct cgcccagcta atgggcagcg gcgtcgggga aattaccgct
    43621 gtggtagtgc gctactacgg cggcatattg cttggcaccg gtgggttagt gaaagcgtat
    43681 ggcggcggcg tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc
    43741 gaatatactt tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag
    43801 tgtgacggca aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt
    43861 ccggcggcga aagtggctga attttccgca aagctggcgg attttagccg tggttcattg
    43921 caattgttag cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag
    43981 agatgcattt tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga
    44041 ccatgattat ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta
    44101 cccagacctt ttttgtcgcc ctcgccattg gctctatgct ttggtggccg aaccgcaaag
    44161 agaaaggtga acttaaatcc cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc
    44221 tgggtagcgt cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg
    44281 atgcgttttt tgaatctttc tctggcctga ccaccacggg ggccactacg ctggtggggc
    44341 tggattcgct ccctcatgcc atcctctttt atcgccagat gctgcaatgg tttggcggga
    44401 tggggatcat cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc
    44461 tctatcgcgc agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg
    44521 aaacggcgaa aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt
    44581 tgtggtttgc cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta
    44641 ttggcggctt ctcgacacat gatgccagta tcggttattt cgatagcccg actattaaca
    44701 ctatcattgc tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt
    44761 taagtgggcg tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg
    44821 tacagtttac gctggtggtt atttgtaccc tcgtactgtg gtttcataat gtctacagtt
    44881 cggcgctgat gacaattaac caggcgtttt tccaggtggt atcgatggcg acaaccgccg
    44941 ggtttacgac tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt
    45001 cagcttttat cggcggttgc gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc
    45061 tgctgctgtt taagcagggg aaccgagagc tgaaacgact ggtgcatccg aacgccgtct
    45121 atagcattaa gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat
    45181 ttttctccgc ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg
    45241 tggatgactt ttctgccttt gcgtcggttg ttgcgacatt gaataacctg gggccagggc
    45301 ttggcgtggt tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg
    45361 ccaacatgct gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt
    45421 tctggcgtga atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg
    45481 acaaacgcgc gagattgcct cctacctggc ttcggaactg aaagaactgg ggatccaggc
    45541 ggatgtcgcc aatgtgcacc gcattgaaga accacagtgg gaaaactatg accgtgtggt
    45601 cattggtgct tctattcgct atggtcacta ccattcagcg ttccaggaat ttgtcaaaaa
    45661 acatgcgacg cggctgaatt cgatgccgag cgccttttac tccgtgaatc tggtggcgcg
    45721 caaaccggag aagcgtactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc
    45781 gcaatggcgt cccgatcgct gtgccgtcat tgccggggcg ctgcgttacc cacgttatcg
    45841 ctggtacgac cgctttatga tcaagctgat tatgaagatg tcaggcggag aaacggatac
    45901 gcgcaaagag gttgtctata ccgattggga gcaagtggcg aatttcgccc gagaaatcgc
    45961 ccatttaacc gacaaaccga cgctgaaata agcataaaga a
//
LOCUS       DAZFZC010000032        45021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFZC010000032 DAZFZC010000000
VERSION     DAZFZC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.032"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            154..258
                     /locus_tag="ACHKXP_002015"
     CDS             154..258
                     /locus_tag="ACHKXP_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647032.1"
                     /translation="MENNSRTMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            516..2714
                     /gene="speF"
                     /locus_tag="ACHKXP_002016"
     CDS             516..2714
                     /gene="speF"
                     /locus_tag="ACHKXP_002016"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO7647033.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2711..4030
                     /gene="potE"
                     /locus_tag="ACHKXP_002017"
     CDS             2711..4030
                     /gene="potE"
                     /locus_tag="ACHKXP_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO7647034.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            4106..4255
                     /locus_tag="ACHKXP_002018"
                     /pseudo
     CDS             4106..4255
                     /locus_tag="ACHKXP_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            4379..5029
                     /locus_tag="ACHKXP_002019"
     CDS             4379..5029
                     /locus_tag="ACHKXP_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HFO7647035.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKDLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(5070..5564)
                     /locus_tag="ACHKXP_002020"
     CDS             complement(5070..5564)
                     /locus_tag="ACHKXP_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647036.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPFRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(5778..7418)
                     /gene="pgm"
                     /locus_tag="ACHKXP_002021"
     CDS             complement(5778..7418)
                     /gene="pgm"
                     /locus_tag="ACHKXP_002021"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO7647037.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(7444..7989)
                     /gene="seqA"
                     /locus_tag="ACHKXP_002022"
     CDS             complement(7444..7989)
                     /gene="seqA"
                     /locus_tag="ACHKXP_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO7647038.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            8174..8938
                     /gene="ybfF"
                     /locus_tag="ACHKXP_002023"
     CDS             8174..8938
                     /gene="ybfF"
                     /locus_tag="ACHKXP_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7647039.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            9078..9371
                     /gene="ybfE"
                     /locus_tag="ACHKXP_002024"
     CDS             9078..9371
                     /gene="ybfE"
                     /locus_tag="ACHKXP_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO7647040.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            9511..10041
                     /gene="fldA"
                     /locus_tag="ACHKXP_002025"
     CDS             9511..10041
                     /gene="fldA"
                     /locus_tag="ACHKXP_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO7647041.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            10251..10337
                     /gene="uof"
                     /locus_tag="ACHKXP_002026"
     CDS             10251..10337
                     /gene="uof"
                     /locus_tag="ACHKXP_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO7647042.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            10330..10776
                     /gene="fur"
                     /locus_tag="ACHKXP_002027"
     CDS             10330..10776
                     /gene="fur"
                     /locus_tag="ACHKXP_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO7647043.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(10860..11186)
                     /gene="chiQ"
                     /locus_tag="ACHKXP_002028"
     CDS             complement(10860..11186)
                     /gene="chiQ"
                     /locus_tag="ACHKXP_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO7647044.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(11236..12642)
                     /gene="chiP"
                     /locus_tag="ACHKXP_002029"
     CDS             complement(11236..12642)
                     /gene="chiP"
                     /locus_tag="ACHKXP_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO7647045.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRAIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(13222..14886)
                     /gene="glnS"
                     /locus_tag="ACHKXP_002030"
     CDS             complement(13222..14886)
                     /gene="glnS"
                     /locus_tag="ACHKXP_002030"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO7647046.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(15154..17100)
                     /gene="nagE"
                     /locus_tag="ACHKXP_002031"
     CDS             complement(15154..17100)
                     /gene="nagE"
                     /locus_tag="ACHKXP_002031"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO7647047.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            17433..18233
                     /gene="nagB"
                     /locus_tag="ACHKXP_002032"
     CDS             17433..18233
                     /gene="nagB"
                     /locus_tag="ACHKXP_002032"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7647048.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            18293..19441
                     /gene="nagA"
                     /locus_tag="ACHKXP_002033"
     CDS             18293..19441
                     /gene="nagA"
                     /locus_tag="ACHKXP_002033"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7647049.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQNANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     KNADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            19450..20670
                     /gene="nagC"
                     /locus_tag="ACHKXP_002034"
                     /gene_synonym="nagR"
     CDS             19450..20670
                     /gene="nagC"
                     /locus_tag="ACHKXP_002034"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO7647050.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEEHFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            20718..21470
                     /gene="nagD"
                     /locus_tag="ACHKXP_002035"
     CDS             20718..21470
                     /gene="nagD"
                     /locus_tag="ACHKXP_002035"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO7647051.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(21521..21619)
                     /locus_tag="ACHKXP_002036"
                     /pseudo
     CDS             complement(21521..21619)
                     /locus_tag="ACHKXP_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21867..23531
                     /gene="asnB"
                     /locus_tag="ACHKXP_002037"
     CDS             21867..23531
                     /gene="asnB"
                     /locus_tag="ACHKXP_002037"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO7647052.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            23911..23987
                     /locus_tag="ACHKXP_002038"
     tRNA            23911..23987
                     /locus_tag="ACHKXP_002038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23945..23947,aa:Met,seq:cat)
     gene            23997..24081
                     /locus_tag="ACHKXP_002039"
     tRNA            23997..24081
                     /locus_tag="ACHKXP_002039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24031..24033,aa:Leu,seq:tag)
     gene            24105..24179
                     /locus_tag="ACHKXP_002040"
     tRNA            24105..24179
                     /locus_tag="ACHKXP_002040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24137..24139,aa:Gln,seq:ttg)
     gene            24214..24288
                     /locus_tag="ACHKXP_002041"
     tRNA            24214..24288
                     /locus_tag="ACHKXP_002041"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24246..24248,aa:Gln,seq:ttg)
     gene            24304..24380
                     /locus_tag="ACHKXP_002042"
     tRNA            24304..24380
                     /locus_tag="ACHKXP_002042"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24338..24340,aa:Met,seq:cat)
     gene            24428..24502
                     /locus_tag="ACHKXP_002043"
     tRNA            24428..24502
                     /locus_tag="ACHKXP_002043"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24460..24462,aa:Gln,seq:ctg)
     gene            complement(24656..25831)
                     /gene="ubiF"
                     /locus_tag="ACHKXP_002044"
     CDS             complement(24656..25831)
                     /gene="ubiF"
                     /locus_tag="ACHKXP_002044"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO7647053.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            25977..27401
                     /gene="miaB"
                     /locus_tag="ACHKXP_002045"
     CDS             25977..27401
                     /gene="miaB"
                     /locus_tag="ACHKXP_002045"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO7647054.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPKMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            27554..28594
                     /gene="ybeZ"
                     /locus_tag="ACHKXP_002046"
     CDS             27554..28594
                     /gene="ybeZ"
                     /locus_tag="ACHKXP_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO7647055.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            28591..29058
                     /gene="ybeY"
                     /locus_tag="ACHKXP_002047"
     CDS             28591..29058
                     /gene="ybeY"
                     /locus_tag="ACHKXP_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO7647056.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQKESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            29148..30026
                     /gene="corC"
                     /locus_tag="ACHKXP_002048"
     CDS             29148..30026
                     /gene="corC"
                     /locus_tag="ACHKXP_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO7647057.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            30051..31589
                     /gene="lnt"
                     /locus_tag="ACHKXP_002049"
     CDS             30051..31589
                     /gene="lnt"
                     /locus_tag="ACHKXP_002049"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO7647058.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(31667..33544)
                     /locus_tag="ACHKXP_002050"
     CDS             complement(31667..33544)
                     /locus_tag="ACHKXP_002050"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO7647059.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLSLSGEPWRLLSSVFLHSSFSHLLMNMFALLVVGAVTERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGTGTHHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREEFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLTLSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKEDFPAGDARGTISAMRFRPENGDVIFAD
                     SQGISRIRVGDQQASIMTQWCSRSVYSVEGISPDGQYLVLVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTLSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            33999..34907
                     /gene="gltI"
                     /locus_tag="ACHKXP_002051"
     CDS             33999..34907
                     /gene="gltI"
                     /locus_tag="ACHKXP_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO7647060.1"
                     /translation="MQLRKPAIAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            35077..35817
                     /gene="gltJ"
                     /locus_tag="ACHKXP_002052"
     CDS             35077..35817
                     /gene="gltJ"
                     /locus_tag="ACHKXP_002052"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO7647061.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            35817..36491
                     /gene="gltK"
                     /locus_tag="ACHKXP_002053"
     CDS             35817..36491
                     /gene="gltK"
                     /locus_tag="ACHKXP_002053"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO7647062.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            36491..37216
                     /gene="gltL"
                     /locus_tag="ACHKXP_002054"
     CDS             36491..37216
                     /gene="gltL"
                     /locus_tag="ACHKXP_002054"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO7647063.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            37334..38269
                     /gene="rihA"
                     /locus_tag="ACHKXP_002055"
     CDS             37334..38269
                     /gene="rihA"
                     /locus_tag="ACHKXP_002055"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO7647064.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTSVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            38353..40023
                     /gene="hscC"
                     /locus_tag="ACHKXP_002056"
     CDS             38353..40023
                     /gene="hscC"
                     /locus_tag="ACHKXP_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO7647065.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDEARECDFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVRVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(40083..41534)
                     /gene="djlC"
                     /locus_tag="ACHKXP_002057"
     CDS             complement(40083..41534)
                     /gene="djlC"
                     /locus_tag="ACHKXP_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO7647066.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPSCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPIALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPSESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(41531..42238)
                     /gene="ybeU"
                     /locus_tag="ACHKXP_002058"
     CDS             complement(41531..42238)
                     /gene="ybeU"
                     /locus_tag="ACHKXP_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7647067.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKAFIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            42340..42894
                     /gene="ybeT"
                     /locus_tag="ACHKXP_002059"
     CDS             42340..42894
                     /gene="ybeT"
                     /locus_tag="ACHKXP_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7647068.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMANYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(42904..44331)
                     /gene="djlB"
                     /locus_tag="ACHKXP_002060"
     CDS             complement(42904..44331)
                     /gene="djlB"
                     /locus_tag="ACHKXP_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO7647069.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMDEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKM
                     HRNLLRWYSAGRLGIPELQGYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHTLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQSIPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(44328..>45021)
                     /gene="ybeR"
                     /locus_tag="ACHKXP_002061"
     CDS             complement(44328..>45021)
                     /gene="ybeR"
                     /locus_tag="ACHKXP_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7647070.1"
                     /translation="QKILFTLSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDD
                     RDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAE
                     CCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQYFAGY
                     TFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQPLADYP
                     TCPETLKDMSDL"
BASE COUNT        11244 a        11381 c        11177 g        11219 t
ORIGIN      
        1 aaaataacaa ttcaatattt tatattactg agtaaaaagc tcatcattaa ataaattaag
       61 attgatcatt ttttattgat caccttcaca gttcaaccgt atttcctgga tagaatatct
      121 tcaccttcat tcacatcagg aaaggtaaat taaatggaaa ataacagccg cactatgccc
      181 catataaggc ggacaactca tattatgaag tttgctcatc gcaatagctt cgactttcac
      241 ttcttcaatg cccgttagtc taccgactaa gggcacttca gcgtacaggt cttcctgact
      301 ctctgtatta caggcatatg cctgtattcc gcagtgctca ttctctgtta acccttgtat
      361 tatcagccac ctgactggta agggtttctg cattctgaaa aaagcaatct acctgattta
      421 tttatcagcg gggtaacttt gcttttttcc ggcacgatcg atttctcatt cgagaaattg
      481 aggacctgct attacctaaa ataaagagat gaaaaatgtc aaaattaaaa attgcggtta
      541 gtgattcttg cccggactgt tttaccacgc agcgagaatg tatctacatt aatgaaagtc
      601 gtaatatcga tgtggcggca atagttttat cgctcaacga tgttacatgc ggaaaactcg
      661 atgaaatcga tgccacgggt tatggcatcc cggtatttat tgctactgaa aatcaagaac
      721 gtgtacccgc agagtatttg ccccgtattt cgggtgtctt tgagaattgc gaatcgcgac
      781 gagaatttta tggtcgccag ttagaaaccg ctgccagcca ttatgaaact caactgcgcc
      841 cacctttctt ccgcgcactg gtcgattatg tcaatcaagg taacagcgcg tttgattgcc
      901 ctggtcatca gggcggcgaa tttttccgtc gccatccggc ggggaatcag tttgtggaat
      961 actttggtga ggcgctgttc cgtgccgact tgtgcaacgc cgacgtagcg atgggcgatc
     1021 tgctgattca cgaaggcgcg ccatgcattg cacagcaaca tgcggcaaaa gtgtttaatg
     1081 ccgataaaac ctacttcgtt ttaaatggca cttcatcttc taacaaagtg gttttaaacg
     1141 ccctgctaac accgggtgat ctggtgctgt ttgatcgcaa taaccacaaa tctaaccacc
     1201 acggagcgtt gctacaggct ggtgcaacac cggtttatct ggaaacggca cgtaacccgt
     1261 atggctttat cggtggcatt gatgcgcact gttttgaaga aagttacctg cgtgagctga
     1321 tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc gctgtgattc
     1381 agttaggcac ctacgacggt acgatttata acgcccgcca agtggtggat aaaattggtc
     1441 atctgtgtga ctacatcctg tttgactcag catgggtcgg ctatgaacag tttattccga
     1501 tgatggcgga ctgttcgccg ctgttgctgg atcttaatga gaacgatccg ggtattctgg
     1561 ttacgcaatc tgtgcataaa caacaggctg gtttttctca gacttcacaa attcataaaa
     1621 aagacagcca catcaaaggg caacagcgtt atgtaccgca caaacgcatg aacaacgcct
     1681 ttatgatgca cgcctccacc agcccgttct atccgctgtt tgccgcactg gatatcaacg
     1741 ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg gtaaatggca
     1801 ttaatgcccg caaactgatc ctcgataact gtcagcatat tcgtccgttc gtacctgaac
     1861 tggtggatgg taaaccctgg cagtcgtatg aaacagcgca aattgcggtt gatctgcgct
     1921 tcttccagtt tgtaccaggg gaacactggc attcttttga aggctatgca gagaatcaat
     1981 actttgtcga tccatgcaaa ctgttgctga caaccccagg tattgatgca cgtaacggcg
     2041 aatatgaagc gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc gaaaatggcg
     2101 tagtgccgga aaaatgcgat cttaactcca tcctcttcct gctgactccg gcagaagata
     2161 tggccaaact tcagcaactt gttgccctgc tggtacgctt cgaaaaactg cttgagtccg
     2221 acgcgccatt agcagaagtg ctaccttcca tctacaaaca gcatgaagag cgctacgccg
     2281 gttataccct gcgtcagttg tgtcaggaaa tgcatgattt gtatgcccgc cacaacgtga
     2341 aacaactgca aaaagagatg ttccgtaagg agcacttccc acgcgtcagc atgaatccgc
     2401 aagaagccaa ctacgcctat ttacgcggtg aagtggaact ggttcgtctg ccggatgcag
     2461 aaggccgtat cgctgccgaa ggtgcgcttc cttatcctcc gggtgtgctg tgtgttgttc
     2521 cgggtgaaat ctggggtggt gctgttctgc gttacttcag cgctctggaa gaagggatca
     2581 acctgctgcc aggttttgca ccggagctgc agggtgtcta tatcgaagaa catgatggtc
     2641 gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc ctgttgaaag
     2701 gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc gtcgttcagt taaccatact
     2761 gacgatggtc aacatgatgg gctccggtat catcatgctg ccgacaaagc ttgccgaagt
     2821 cgggacaatc tcaattatct cctggctggt gacagccgta ggctcaatgg cactggcatg
     2881 ggcattcgca aaatgcggta tgttcagccg taaatcaggc ggtatgggcg gttatgccga
     2941 gtatgctttc ggtaaatccg gtaactttat ggcgaactat acctacggcg tctcactgct
     3001 gattgctaac gtcgcgattg ctatttcggc ggttggttac ggcaccgaac tgctcggcgc
     3061 gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga tttgtaccgt
     3121 ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt agcattaccg tgtggggggt
     3181 cattattccg gtcgtcggtc tgtgcattat tggctggttc tggtttagcc cgacgctgta
     3241 cgttgattcc tggaatccgc atcatgcacc gttcttcagt gcggtaggtt cttccatcgc
     3301 tatgacgctg tgggcttttc ttggtctgga gtctgcgtgt gcgaatactg atgtagtgga
     3361 aaacccggaa cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat
     3421 ttatatcgtc tccaccaacg tgattgccgg gattgtgcca aatatggagc tggcaaattc
     3481 aacggcacca tttggtctgg ccttcgcgca gatgttcacg ccggaagtgg gtaaagtcat
     3541 tatggcgctg atggtgatgt cctgctgcgg ttcgctactt ggctggcagt tcaccattgc
     3601 ccaggtgttt aaatcttcat ctgatgaagg ctacttccct aaaattttct cccgtgtaac
     3661 caaagtggat gcaccggtgc agggaatgtt gaccattgtg attattcaga gtggattggc
     3721 actgatgacc attagcccgt cgctaaacag tcagttcaac gtgctggtta acctggccgt
     3781 ggtgaccaat atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt
     3841 cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat
     3901 gtatagcttt tatgcgctgt actcatccgg ggaagaagcc atgctgtacg gttccatcgt
     3961 gaccttcctt ggttggacac tgtatggtct ggtatcacca cgctttgaac tgaaaaataa
     4021 acacggttaa taaaacaaag taaaaaaggg cgattgaatg atcgcccttt ctgtttgttg
     4081 gcaaatagtt cgttatttat gccgcatccg gcagatgtag ggtctaaacg cgatcgaaca
     4141 cagcaactat ttgcgtaatt atatggggca tctgcgacaa aaactggaac aggatccggc
     4201 ccggcctcgc cattttatta ctgaaaccgg tattgggtat cggtttatgc tttgaaaaaa
     4261 gtggcaatgt atggataaag tcatttttta ttcaaatata aggaacttaa tatttaaaaa
     4321 atgttccata caattcctca tcataaatga caccgtagca cacaaggagc aagtagcgat
     4381 gcgtgaaatg aatcagttag taaatactga agattcagcc tggccaataa tccagaactg
     4441 gttaaaagac gccacaaacc ataccgaact cttaccggtt aataaagatt tagccgaaac
     4501 cgcactttat caattacagg tgacaacaaa atcaccgatg ggtgcgcttg tctatggctc
     4561 cggaggtctg ctgattgata atggttggtt acgcatagca ggatctggtc acccccggtt
     4621 gccacgagat ccagtaagtt ggacacaacg ccctgaattc gcaggcgtgc gggctttacc
     4681 catcgctgac gatgtcgcag ggggaatttt tgctcttaat ggtggcgatc ttggggaaga
     4741 tactggctgc gtttattatt ttgcgccaga tacgctgaat tgggaaagtc tggaagttgg
     4801 atattctgag tttcttcagt gggcattatc cggtgattta gataccttct atgaaaatgt
     4861 acgatggcag caatggcgag aagacgtaat aaaattatcg gcaacggaag ccttcacgtt
     4921 ctaccctttc ttatgggtac aaagtgaaga agcaagaaca agaaaagtta tttcgcttac
     4981 tgaactctgg gaaatgcagt atcaaatgaa agaaacattc acgcaataag acatgttcag
     5041 gtccgggttt cattcccgga ctcactattt taatgtgtat ttatcgcttt caattcttgc
     5101 tggcaatttt ctgactggca gcgtcgtaac tcaataatcc cgacatgcat catcgagcca
     5161 aaaaatccgc tttcgctgac ataatcctca ttgactatcg gtcgagtgcc gggcttgccc
     5221 tggcaaaccc gcataaaaaa tgaggtgtag cattgccaac cgggatttaa accgaccttt
     5281 tcttgtgctg acatattatg aagataagta agttcttttt cgttcccgcc tgacaactgc
     5341 cagcggcggg tattatcagg taagttatca ccacggcaat ttgtcttaca ccggaaaggt
     5401 gcaatattta ccgcaatgct acccgttaat cctctggttt tatctttagg ggtcagtaac
     5461 cagtcgccag ctaatgatga ttgttcaggt tgggcgcatg cagtcagcag taatgtggcg
     5521 gtaattagtg acactatgac gaccagactc ctgttggtct tcattgggat ttccttgagc
     5581 tactcagttt gttgacaaag tgttcgttgt ttttgccgga tgcggcgtga acgccttatc
     5641 cggcctacaa aatcgcttaa attcaatata ttgcaccatc ctcgtaggcc tgataagcgt
     5701 agcgcatcag gcaattctgt gtttgtcata cgtaaaaaag ggcgatcttg cgaccgccct
     5761 ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct
     5821 ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat cttatatgcg
     5881 tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg
     5941 ccaatagaag caccgttgcc cggagcagca gtcaggcgcg cggtgatcgg gtcacctgcc
     6001 agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt ttgtgcggaa
     6061 gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc cagttcgttg
     6121 tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacatg
     6181 atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagcccct
     6241 gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac aaaccatttg
     6301 aaacctaccg ggacttctac cagcttacga cccaagtcat tgaccacacg gtcgatcatc
     6361 gcagaagaaa ccagcgtttt accaacggca acatctttgc cccactgcgg acgatgctgg
     6421 aacaggtaat tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg
     6481 acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc
     6541 agtgccagca ggcccgccat cgcacactcg gaggagcagt ccatacggat cgcgccgtct
     6601 ttatcaaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat agtcaggttg
     6661 aggttgtaat actcgccaat gcgcttccag tattcgatac cggaaccgcc cagcggatca
     6721 acgccaagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagccct
     6781 tccacgaacg gctgcaccag atcctgctct ttcacatgac cggatgccat cgcttcgtcg
     6841 agggagatac gcttcacgcc tttcaggcca tcggccagca gtgcgttggc acgatcttcc
     6901 accactttag tgacgttggt atcagccggg ccaccatttg gcggattgta cttgatacca
     6961 ccatcttccg gcgggttatg ggacggtgta ataacgatac cgtctgccag cgggccacct
     7021 tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggggtgaa gccattgttt
     7081 tcctgcacaa taacatctac gccgttcgct gccagcactt ccagaacgga aataaatgca
     7141 ggttcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat accgtttttc
     7201 gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg
     7261 cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct
     7321 gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc
     7381 tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac
     7441 gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca tcgactgcat
     7501 gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg
     7561 cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc
     7621 tgccgcaaag tagacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg
     7681 ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg
     7741 ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt
     7801 tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg
     7861 agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct
     7921 ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat
     7981 cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccag gagtatatga
     8041 ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt
     8101 atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa
     8161 aaagaacttt gccatgaaat tgaatatccg cgcgcaaact gcacaaaacc agcacaataa
     8221 ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg gcgtactggc
     8281 tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc
     8341 accgagagag ccggtaatga attacccggc gatggcgcag gatcttgttg ataccctgga
     8401 tgcactgcag atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat
     8461 ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg atatcgcacc
     8521 ggtcgactat cacgtacgcc gtcatgatga gattttcgca gctatcaacg cggtcagtga
     8581 atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc ttaatgaaga
     8641 aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc
     8701 ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca
     8761 ccctgccctg tttatccctg gtggtaattc tccgtatgtt agcgagcagt accgtgatga
     8821 tttactggca caatttccac aggcacgggc gcatgtcatt gcaggcgcgg gtcactgggt
     8881 ccatgctgaa aaaccagatg cggtattacg cgctatccgt cgctatctca atgattaacg
     8941 caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg gcgtgactca
     9001 tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg attgtttccc
     9061 ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg
     9121 cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gaacagctgc
     9181 gtattaataa acgcaaccaa ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac
     9241 tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggaatcg cgcaatatga
     9301 gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg
     9361 gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt
     9421 ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat
     9481 ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc
     9541 gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt
     9601 gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta tgacattctg
     9661 ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc
     9721 ccgactctcg aagagattga tttcaatggc aaactggttg cgctgtttgg ttgtggtgac
     9781 caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga catcattgaa
     9841 ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt cgaagcatca
     9901 aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga ccgtcagccg
     9961 gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga gttgcatctc
    10021 gacgaaattc tcaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata
    10081 gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctgtacaa
    10141 tgtcccggtg ttcaagtggc cttgccgttg taaatgtaag ctatgccacg tttttattaa
    10201 caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca
    10261 ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta gtaacaggac
    10321 agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt
    10381 cctcgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa
    10441 gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc
    10501 gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga aggcggtaaa
    10561 tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatctgcct cgactgcggc
    10621 aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat tgccgcaaaa
    10681 catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat
    10741 tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa
    10801 cctgcgggtt ggctttttta tgcaagggaa ataagaaaag cgagagttac agctctcact
    10861 tatttgttat tactgcgaat ttcctgccag actttatcac aatcggcttt caccgcctga
    10921 tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg tacactttcc
    10981 tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag
    11041 gcttcaattt tttctggcga accttgtgcg gtgttgatac aggcgctgta tgcctctttc
    11101 agacggctgt cttcctgtgg cgcggttgat tgcgcacaag ccaccagccc cgatgccatc
    11161 atggcgatga gaatcagttt tttcatagca aattcctcaa atatgcggca ggcacaaaac
    11221 ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg
    11281 ctcatcctgg aagatgttgc cgtaaccacc gccccagctt gggatgtcgg agtggttgtc
    11341 gtattcggtg aaatgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta
    11401 gaccgcatcc aggctgtatg cagactcttc aatagcccgg tttttgtcgt agtacgcatc
    11461 cggattgctc tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat
    11521 ggcgaagcct ggaagattcc agtttttcag gtcatacatc gcaccgaaga agactgcttt
    11581 ttcgccgttg gcgttgaagt cagaacggtt gtcccaccag atatccaggc gaccgtttga
    11641 ggaagcgtag gttggagtca tacgttgcag gaagtatccc tgctgaccgt cagccttaac
    11701 ccaggtgcct tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc
    11761 ctgcagccag gcggtgccgt cataaaggtc gttgacgctg cgatcgtcaa ctttatcgcg
    11821 ggtaccgtag aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct
    11881 ggctttggca aaatattgat cgatataccc ttccgcctga ccaaaagccg cttccagtac
    11941 gaagttattt ttgaagtcgt atttcgcccc aagggagtgc agataatcaa ctttggtggt
    12001 tttatcgttc tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt
    12061 ccacatgtag gagaaactca acgcaccggc atcgccgtaa tcaaaattcg ctccggcttc
    12121 cgcaccctga taagtacccg gcataaagct ccagtgaggc gctaacagcg tctgaccggt
    12181 tggctgaata taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgctgcttt
    12241 atacaggctt ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt
    12301 tgaaaacgcg atttcgttcg ggtgagagct gtcgccgttt tccgccattt caatcgccgt
    12361 aaacgcggca atatcaaggc cgaacatatc agcagcataa ccagactgaa aatcgaggtt
    12421 ggcattccag gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt
    12481 acggtcgcgt tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa
    12541 tccttcggcg cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcgat
    12601 agccagcgcc agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac
    12661 gtataaattg ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaatttaa aaagcagcgt
    12721 tgagaaaagt ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct
    12781 ctttctgtaa ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta
    12841 caaggcttaa gtttgcggtt gaaaggcttg cagaagaata aaagcgtgaa tttccagcaa
    12901 gatgaaagcg gttacatttt atttaattat gttttgttat tttaaattta ttttatggat
    12961 aaattgcgaa gcaggattaa ttcgcgtaga aaagtagaaa aaaacgccac agagcgcggc
    13021 gttttggatt gtttgctgtt tcttgccgga tgcggcgtga acgccttatc cggcctacaa
    13081 aagcacgcaa actcaatata ttgcagagat catgtaggcc tgataagcgt agcgcatcag
    13141 gcaatttagc gtttgaaact gtttatctgt tccttgccgg atgcgacgta aacgccccat
    13201 ccggcatagc gaaacttaaa attactcgcc tactttcgcc caggtatcac gcagcccaac
    13261 ggtgcggtta aataccggtt tttccgccgt agaatggcgg ctatcgaggc agaagtaacc
    13321 ttcacgctca aactggaatg ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa
    13381 gccctgtttg atcaccagcg attccgggtt aatcaccgac aggaaatcat ccgcagcacc
    13441 tgggttaggc acgctgaaca gacggtcata caaacggatt tcaaccggca gcgcatgtgc
    13501 cgcgctcacc cagtgaataa cacctttgac tttacgacca tctgccggat ctttgcttaa
    13561 ggtatcggcg tcataagtac agaagatggt ggtgatatta ccttcggcat ctttctcgac
    13621 gcgttctgcc ttaatcacat aagcattacg cagacgcact tctttaccca gcaccagacg
    13681 tttgtactgc ttgttagctt cttcgcggaa atcggcgcga tcaatccaaa tctcaccgct
    13741 aaacggcacc tgacggctgc ccatttccgg tttgttcgga tggttcggca tggtaaccat
    13801 ttcgccttcg ccctgatagt tttcgataac cagtttcacc ggatcgataa ccgccattgc
    13861 gcgcggcgca ttttcgttga gatcttcacg gatgcaggat tccagcgacg ccatctcaat
    13921 ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac tcacgaatag aagccgcagt
    13981 gtaaccacga cgacgcagac cggaaatggt cggcatacgc gggtcatccc agccttcaac
    14041 gtgcttgtcg gtcaccagca ggttcaactt acgcttggac atcacggtgt attccagatt
    14101 caggcgcgag aactcatact ggcgcgggtg aacaggaatc gtgatgttgt ccagtaccca
    14161 gtcgtacaga cgacggttgt cctggaactc aagcgtacac agagagtgcg taataccttc
    14221 cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg tagatgcacc acttgttgcc
    14281 agtctggtgg tgttcagcaa atttaatacg gtacagcacc ggatcgcgca tcacgataaa
    14341 cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc
    14401 acgcattttt tcgaacagcg ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt
    14461 tttacccggt tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg tcagttcatc
    14521 aacgtacgcc aggcctttat tgatcagttc gatcgcatag gcgtggagct gatcaaaata
    14581 atcggaggag taacggacgt taccagacca gtgaaaacct aaccactcta cgtcgttttt
    14641 gatcgactca acatactcga tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt
    14701 gcactggcct ttatagtcct gggcgatccc gaagttcagg cagatagatt tcgcatggcc
    14761 aatatgcaga tagccattcg gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc
    14821 actggccaga tcttcatcga tgatctgacg gataaagtta gtcgggcggg cttctgcctc
    14881 actcatcgtg gattcctcaa agcgtaaaca acgtataacg gcgtatgatc ttataagcgg
    14941 gacaggctga caactgttag ttagtgaaaa tcgaaaatct cactgagatt atggggatga
    15001 ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt ttgccagtcc tgcgtcattg
    15061 ttggatgcga cgcttaagcg tcttatccaa cctggactga gcttttgtag gcctgataag
    15121 acgcgtcaag cgtcgcatca ggcataaagc agattacttt ttgatttcat acagcggtgt
    15181 ttgacccgcc acaacatggc cctgagcttt aatgatcaag ccactgaaat cgtcgatatt
    15241 gctgcaaacc accgggctaa tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat
    15301 ttccagaatc ggttgccctg cgcttacctg cgcaccctct tccaccagac gtttaaagcc
    15361 tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc
    15421 ggtttccagg cagaacgcgt ggttggtgtt gaagattttc acgattgtcc ctgcggctgg
    15481 tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc
    15541 gaatgcttcg tcaggaacct gatccagtgc cacgacatca ccggtaatcg gcgataccag
    15601 ctccgcgata gataccgcgt ttggtacagc ctgcggtttt gctacaggcg cggcagttgc
    15661 cggagttgct tcagctgacg cagctgctac cggaccgcgg gcaacgactt tcttcatcgc
    15721 atcgccgatg gattctgctt tcgcgccaac aatcacctga atagtctgtt tgttcagttt
    15781 tactacacca gaagcaccca gacgtttaca catcgtatcg ttaacgcggg cagagtcagc
    15841 cactgtaagg cgcagacggg tgatacaggc gtcaattgct ttcaggttgt cagtgccgcc
    15901 aaccgcagca atatagttgg tcgccagttg attgaggcct tcttcagtgt tgctgttggc
    15961 ttcttcagta acgatctcgt cttctttatc ttcacgaccc ggcgttttca ggttgaacat
    16021 gcggataacc aaactgaaca ccacgaagta gatagcgaag aagacaacac ccatcaccag
    16081 cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt agtcgatagc
    16141 ccccgcagag aaagagaagc cagcatggat acccagcagt gttgccacaa acaggctgat
    16201 accggtcagc agtgcgtgca ggaggtacag cagcggtgca aggaacatga acaggaattc
    16261 cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcatcccgcc
    16321 aaccatcgga cgacgctctt tcggtgctgc gaagtacatc gccagcgccg cacccggcag
    16381 accgaacatc ataatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccggc
    16441 atagaagcgg ttgatatcac cgtggaaaac cgtacccgcc gcgttggtga attcaccaat
    16501 ctggaaccag gcgatggtgt tcagcacctg atgcagaccg gttgggatca gcagacggtt
    16561 gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact cgcctcctgc
    16621 atggatagcg tgctgtaccg gcggccagac gtaaccaaaa atggccgcca gcaccaggca
    16681 gaagaagccg gtggcaatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg
    16741 cagtttaata tcggaccaac ggttataggc tgcgccacca accagaccgg taatgatacc
    16801 cgccagtaca cccatgttta tttctgggtt gatggtcacc atcgctttgg ttaacacaaa
    16861 gtaacctacc gcacccgcca gcgccgccgc acctgcgctg tctttcgacc agctggatgc
    16921 cacaccgatg gcgaagatta atgcgaggtt atcaaaaatc gcaccgcccg cctgggcaat
    16981 aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagtgccg ccaccggcag
    17041 caccgcgata gggagctgta acgccctacc gagtcgctgg aaaaaaccta aaatattcat
    17101 cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc
    17161 aaattgtact gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga
    17221 aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc
    17281 gttatcatca ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc
    17341 aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc
    17401 aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg
    17461 aacaggtcgg taaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccga
    17521 ctgccgatcg tccgtttgtt ctgggcctgc caactggcgg cacgccaatg accacctata
    17581 aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca
    17641 acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc
    17701 accgtaattt cttcgatcac gttgatattc cagcagaaaa catcaacctt ctcaacggca
    17761 acgccccgga tatcgacgcc gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa
    17821 aaattcatct gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg
    17881 cgtcttctct ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga
    17941 actctcgttt ctttgataac gatgtcaatc aggtgccaaa atatgccctg actgtcggtg
    18001 ttggtacatt gctggatgcc gaagaagtga tgattctggt gctgggtagc cagaaagcac
    18061 tggcactgca ggccgccgtt gaaggttgcg tgaaccacat gtggaccatc agctgtctgc
    18121 aactgcatcc aaaagcgatc atggtatgcg atgaaccttc caccatggag ctgaaagtta
    18181 agactttaag atatttcaat gaattagaag cagaaaatat caaaggtctg taattgttat
    18241 ccctgccctg gctccttgct cagggcaata ttttttaaaa tcgggggtca gaatgtatgc
    18301 attaacccag ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat
    18361 cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca
    18421 acgttcactg aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg
    18481 cggcggcgta cagtttaacg acaccgctga agcggtcagc gtggaaacgc tggaaatcat
    18541 gcagaacgcc aatgagaaat caggctgtac taactatctg ccgacgctta tcaccaccag
    18601 cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa
    18661 tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca
    18721 taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtaagaacgc
    18781 cgacgtcatt actaaagtga ccctggcacc ggaaatggtt cctgcagaag tcatcagcaa
    18841 actggcaaat gccgggattg tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc
    18901 gaaagccggt ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta
    18961 tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg acatttattg
    19021 cggtattatc gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaaacgcct
    19081 gaaaggcgac aaactgtgtc tggttaccga cgccaccgcg ccagcaggtg ccaacattga
    19141 acagttcatt tttgcgggta aaacaatata ctaccgtaac ggactttgtg tggatgagaa
    19201 cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca
    19261 ttgcggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat
    19321 tggcgttgag aaacgtctcg gcacactcgc cgcaggtaaa gtagccaacc tgaccgcatt
    19381 cacacctgat tttaaaatca ccaggaccat cgttaacggt aacgaggtcg taactcaata
    19441 agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag
    19501 cttaacagcg cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag
    19561 attgccgagc aaagccagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc
    19621 gaacgcgggc tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc
    19681 tctatcgtca ccgaaacccg caatttccac gcaatcggtg tacggcttgg tcgtcacgac
    19741 gccaccatta ctctgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg
    19801 ccggaacgta cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt
    19861 gatagctacc aacgcaagct gcgcgagctg atcgccattt ccgtgatcct gcctggactt
    19921 gttgacccgg acagcggcaa aattcattac atgccgcata ttcaggtaga aaactggggg
    19981 ctggtagaag ctctggaaga acattttaaa gtgacctgtt tcgttggtca cgatatccgt
    20041 agtctggcgc tggcggagca ctacttcggt gcaagtcagg attgcgaaga ctccatttta
    20101 gtgcgtgtcc atcgcggaac cggggccgga atcatctcta acgggcgcat ttttattggc
    20161 cgcaacggca acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc
    20221 cactgcggca actttggctg cctggaaact atcgccgcca acgctgccat tgaacaacgg
    20281 gtgttgaatc tgttaaagca gggctaccag agccgcgtgc cgctggacga ctgcaccatc
    20341 aaaactatct gcaaagccgc gaacaaaggc gatagtctgg cgtcggaagt aattgagtat
    20401 gtcggtcgtc atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa
    20461 attgttattg ccggtgaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc
    20521 tgcattaata cccaggcgct gaaggcgttt cgcactaatc tgccggtggt acgttctgag
    20581 ctggatcacc gctcggcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt
    20641 attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc
    20701 aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga
    20761 tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag
    20821 gcttgccgct ggtgttgctg accaactatc cttcgcagac tgggcaagat ctggcgaacc
    20881 gctttgccac cgcaggtgtc gatgtacctg acagcgtgtt ttatacctct gcgatggcga
    20941 ctgccgattt cctgcgtcgc caggaaggca agaaagcgta tgtggtgggc gaaggcgcac
    21001 tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct gatttcgtga
    21061 ttgttggcga aacgcgttcc tacaactggg acatgatgca taaagcagcc tatttcgtcg
    21121 ctaacggtgc acgttttatc gccaccaatc cggacaccca cgggcgcggt ttttatcccg
    21181 cttgtggcgc gttgtgtgca gggattgaga aaatctccgg gcgcaaaccg ttctatgttg
    21241 gtaagcccag cccgtggatc atccgcgcgg cattaaacaa aatgcaggcg cattcggaag
    21301 aaacggtgat tgtcggcgat aacctgcgta ccgatattct ggcgggcttc caggcaggtc
    21361 tggagacgat tctggtgctt tctggtgttt cgtcgctcga cgatatcgac agtatgccgt
    21421 tccgcccaag ttggatttac ccatcggtcg ctgaaatcga cgttatctga atagcaacca
    21481 cgtctgcgga cgtggttggc tctgatgaca aacgccaaac tgcctgatgc gctacgctta
    21541 tcaggcctac gcagctcctg caatatattg aattttcatg cttttgtagg ccggataagg
    21601 cgttcacgcc gcatccggca tgaacaaatt gcactttgtc agcaatttgg cccccgtaaa
    21661 atataaccat tcaataaaac ccgctaaaaa ttatcgattc atcaataaat acatccttac
    21721 accagacctt ttttcaccaa tcgacaatca ccattacgtt ttttattttt tcaccgccaa
    21781 atcgcttgcg cacagtgctg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
    21841 caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa
    21901 cagacgcagt tgagctgcgt aagaaagccc tcgagctgtc acgcctgatg cgtcatcgtg
    21961 gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgcccac gaacgtctgt
    22021 caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa acccacgtac
    22081 tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgccgaatat ggcgatcgtt
    22141 accagttcca gaccgggtct gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc
    22201 cggaatttct cgacgacttg cagggcatgt ttgcctttgc actgtacgac agcgaaaaag
    22261 atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg gggtatgacg
    22321 aacacggtca gctgtatgtg gcctcagaaa tgaaagcgct ggtgccagtt tgccgcacga
    22381 ttaaagagtt cccggcgggg agctatttgt ggagccagga cggcgaaatc cgttcttact
    22441 atcatcgcga ctggttcgac tacgatgcgg tgaaagataa cgtgaccgac aaaaacgagc
    22501 tgcgtcaggc actggaagat tcagttaaaa gccatctgat gtctgatgtg ccttacggtg
    22561 tgctgctttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag aaatacgcag
    22621 cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcagtta cactcctttg
    22681 ctgtaggtct gccgggttca ccggatctga aagcagccca ggaagtggca aaccatctgg
    22741 gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg
    22801 tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca ccgatgtatt
    22861 taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt gaaggttctg
    22921 atgaagtgtt cggcggttat ctttacttcc acaaagcacc gaatgccaaa gaactgcatg
    22981 aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgt gccaacaaag
    23041 cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg
    23101 tggcgatgcg tattaaccca caggataaaa tgtgcggtaa tggcaaaatg gaaaaacaca
    23161 tcctgcgtga atgttttgaa gcgtatctgc ctgcaagcgt ggcctggcgg cagaaagagc
    23221 agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gctgcgcagc
    23281 aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac acgccaacct
    23341 ctaaagaagc gtacttgtat cgggagatct ttgaagaact attcccgctt ccgagcgccg
    23401 ctgagtgcgt gccgggcggt ccttccgtcg cttgttcttc cgctaaagcg atcgaatggg
    23461 atgaagcgtt caagaaaatg gacgatccgt ctggtcgcgc ggttggtgtt caccagtcgg
    23521 cgtataagta agaaatgcga aacagccccg gtgaaatccg gggctgtttc agttattgag
    23581 gtgcttgttg attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg
    23641 acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta ttgttaaatt
    23701 ccgaatttca tcctgtatga caaaagaatg gctgtagggt gggaaaaaac acaaagtaaa
    23761 caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt cactttcgag
    23821 caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat gcgccccgca
    23881 acgccgataa ggtatcgcga aaaaaaagat ggctacgtag ctcagttggt tagagcacat
    23941 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct ttttttgcgg
    24001 gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt
    24061 caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc
    24121 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
    24181 cttcttcgag taagcggttc accgcccggt tattggggta tcgccaagcg gtaaggcacc
    24241 ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc gaagaaacaa
    24301 tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc
    24361 gaatcccgtc gtagccacca aattctgaat gtgtcgaata tgttcggcaa attcaaaacc
    24421 aatttgttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
    24481 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
    24541 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
    24601 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
    24661 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctcc gccgccatta
    24721 acccgagatt acgcataaaa cgcagtggtg gcagattatt gctgaatccg gcataaaaca
    24781 gatccatacc gctttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
    24841 ggacaggata actggcccac gcttcgccgt agctgcgggc gttgaccaga acatcaatca
    24901 gggcatcgac atcacgataa ccaagattca ctccctgccc cgccagcgga tggatggtat
    24961 gcgcggcatc gcccaccagc gcaagccctg gctgcacgta ctgcaacgca tggcgacgcg
    25021 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
    25081 tcgcgatttc cgcctggagc tgtgccatat tcatattctg caactggcga atacgcgccg
    25141 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
    25201 cgtccggagt aaattgctgc caggtgctgt cgccgggatc gttctcgcac tggacgctaa
    25261 tcaacatgca cgactgcgca tactgccatg catgaacgcc aattcccgcc atctgccgca
    25321 cctgcgaatt tgcgccgtcg gcaccaatca ccagcttcgc gcgaatcact tcaccgcctt
    25381 tcagctccag ctcctgaaga tcatcatggc gatgcagcgc aatcagcgag cctggcacac
    25441 gtaacgttac tttcggatgc gcttccagcg cctgccacaa cgcctgttgc aggacagtgt
    25501 tttccaccat atagccaagc agcggtagct taagttcagc ggcgtcaaac accacatgcg
    25561 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
    25621 catcccagac ccctaaccct ttaagcaatg ataccgaagc cgcgctgatc gccgagatcc
    25681 gcacgtccgg ttggctatca gcgacaaacg gcgctggttc tgcgtgctcg atcaccgtta
    25741 ccgcaaatcc gtgctgtgcc agccccagcg ccagtgcgcc gccgaccatt cctccgccga
    25801 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
    25861 aagtttacag gatttttacc cctaagggct gataacgctc atactggtca caacggcagc
    25921 aaagcattac actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
    25981 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
    26041 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
    26101 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
    26161 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
    26221 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
    26281 tttttgggcc gcaaacgctg caccgtctgc cgaagatgat caactccgtg cgtggcgacc
    26341 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
    26401 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
    26461 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtcc gacgatattc
    26521 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
    26581 acgtgaacgc ctggcgtggt gaaaactacg acggcaccac cggatcgttt gccgatctgt
    26641 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
    26701 tcgaattcac cgacgatatc atcgaagtgt accgcgacac gccggagctg gtgagcttcc
    26761 tgcacctgcc ggtacagagt ggttccgatc gcattctgaa cctgatgggc cgtacccata
    26821 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgcccg gatattcaga
    26881 tcagttctga cttcatcgtc ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
    26941 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
    27001 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc
    27061 tgtatattct gcaagaacgt atcaatcagc aagcgatggc gtggagccgc cggatgctcg
    27121 gcaccaccca gcgtattctg gtagaaggta catcgcgtaa gagcatcatg gagctttccg
    27181 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
    27241 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
    27301 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
    27361 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
    27421 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
    27481 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
    27541 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
    27601 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
    27661 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
    27721 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
    27781 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcaa gggtactgga
    27841 gcaaagcgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
    27901 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
    27961 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tcgctgcagc
    28021 agttgatgcc ctggagcgtc aggaaattcg ccgtattctg ctgactcgtc cggcggtcga
    28081 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
    28141 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagtcg agaaactgat
    28201 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaatga
    28261 cgcctttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
    28321 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
    28381 gccgcgtaat actaaatcag gcttacgtca tgccatcgaa gtgctggccg atgtcgaaga
    28441 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt cacccggtgg tggcgcgtat
    28501 cgttaacgct tatgaagcct gggaagaagc agaacaaaaa cgaaaagcgg cgctggcggc
    28561 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
    28621 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcagacatg gctgaatgcg
    28681 gtgatcccgc agtttcagaa agaatcggaa gtgactattc gcgtggtcga taccgccgaa
    28741 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
    28801 ccgtttgaag tgccgcctgg catggagatg tcgctactgg gcgatctggt tatctgccgt
    28861 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
    28921 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
    28981 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
    29041 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
    29101 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
    29161 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
    29221 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
    29281 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
    29341 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
    29401 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
    29461 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
    29521 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
    29581 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
    29641 tggcgatcgt tattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
    29701 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
    29761 gtcaactgag tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg
    29821 aagcgttcgg cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc
    29881 aggcgtttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
    29941 aagtggcgat ggccgatagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
    30001 caccccagcc aaagctggat gaataaaacc gaaactggat agataactac atggcttttg
    30061 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
    30121 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ctgatggggc
    30181 ttcaggcgtt gacctttaac cgtcgcccac tccagtctgc cgctattggc ttttgctggg
    30241 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
    30301 ggatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
    30361 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
    30421 tggcgattgc tgcccctgcc ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
    30481 ccggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
    30541 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
    30601 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
    30661 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
    30721 tttcgatggt tcagggcgat atcccacaat cgctgaaatg ggacgaaggc cagcttctta
    30781 atacgctgaa gatttactac aacgcaacgg caccgctgat gggcaaatca tcgttgatta
    30841 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
    30901 tggacggtga gttgcgtgat aaaggtagct cgctggtcac cgggattgtc gacgcgcgtc
    30961 tcaataagca gaaccgctac gatacctaca acaccatcat cacgctgggt aaaggtgcac
    31021 cgtacagcta cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt
    31081 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
    31141 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
    31201 cggctatttg ctacgagatc attcttggtg agcaggtgcg cgataatttc cgcccggata
    31261 ccgactatct gctgaccatc tctaacgatg cgtggtttgg taagtctatt ggtccgtggc
    31321 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccgcta ctgcgcagca
    31381 ccaataacgg tattacggcg gtgattggcc cgcaggggga gattcaggcg atgatcccgc
    31441 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtacg
    31501 cacgtaccgg caactggccg ctgtgggtgc tgacggcact gtttggtttt gccgctgtgc
    31561 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    31621 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    31681 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagagcg
    31741 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    31801 aaaaacgaag gtgattaacg tagctggcag gataaacacc aataatctgc ccggcattaa
    31861 tattgagcag gatgacataa ccttgcaggc catatgacac caacactaaa tattgaccgt
    31921 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    31981 cttgttgatc cccaactctt atacgtgaaa tcccctggct gtcggcaaaa attacatccc
    32041 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    32101 cttctttctg atgcgtgatg gtattgatgc gtacaatgtc acctctgacc ataccgggta
    32161 tcaacaatat tttttcatcc ggggaaagtg ccataaccca ggcaccaggt ttattactcg
    32221 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    32281 caatcgctga cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    32341 agaggataag actgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccccgtcg
    32401 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaagc aacgtttcat
    32461 ccgggcttag cgtcagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    32521 acttttcttt cgctatgggg gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    32581 aaacaacaat gctgttggtg ttttcaattg cggcatacaa cgtattacca tcccgactga
    32641 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    32701 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgttgttttt
    32761 tttgtcgtcg ctcttgttca atctcctgcg gataaaactc ttcacgcagg ctttgcctga
    32821 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    32881 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    32941 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    33001 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    33061 ctcgccgctg gtttttatgg tgtgtgcccg tacctgaagc aagctgtgtt gctatcgcag
    33121 cgccagctat tcccataatt gccccggatg ccccaacgct gatgactatc tgatcactat
    33181 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaaataa
    33241 tcagaagtcg ccatttcccc agtatccgtt ccgtcactgc ccccaccacc aggagtgcaa
    33301 acatattcat cagcaaatgg gaaaaactac tgtgcagaaa gacactgctc aataagcgcc
    33361 agggttcacc cgacaacgag agtgaagcaa cgttagcacc gagatggatg agtgcggatt
    33421 gatcgacttc ttcataactc atccattgtg cgaggtaaca aaatatccca acacagagcg
    33481 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    33541 acataaggtg tttcttcctt gaaaagacag ataaaaagca tttttcccgt gtgcagttat
    33601 catcccggca aacgtcccat accgaaaagt taaccctgtc cgaaatatga ctttttcagc
    33661 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    33721 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    33781 cggtagcacc aaaagagtgc attttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    33841 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    33901 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    33961 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    34021 tagcaatcct ggccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    34081 cagcgggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    34141 aatcttcagt gcctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    34201 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttacagg
    34261 tgaaactgat tccgattacc tcacaaaacc gtattcctct gctgcaaaac ggcactttcg
    34321 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    34381 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    34441 ttgccgacct gaaaggcaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    34501 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcacg
    34561 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    34621 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc
    34681 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga tgatccgcag ttcaaaaagc
    34741 tgatggatga cactatcgct caggtgcaga cttccggtga agcggaaaaa tggtttgata
    34801 aatggttcaa aaatccaatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    34861 aaatgaaagc actgttcaaa gaaccgaatg acaaagcact gaactaatta caagaaccag
    34921 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    34981 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    35041 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    35101 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    35161 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    35221 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    35281 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    35341 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    35401 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    35461 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    35521 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    35581 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    35641 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    35701 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    35761 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    35821 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    35881 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    35941 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    36001 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    36061 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    36121 tggcgttttc aatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    36181 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    36241 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    36301 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    36361 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctatttgccg
    36421 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    36481 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    36541 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    36601 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    36661 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    36721 ccgcgtcggg atggtgttcc agcattttga gttgttcccc catttgtcga ttattgaaaa
    36781 cctgactctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    36841 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    36901 ttccggcggt cagcagcagc gtgtggcgat cgctcgcgca ttgtgtatgg atcctattgc
    36961 catgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    37021 cgtgatggtg gaactggcaa acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    37081 ttttgcccgt aaagtggcga atcgggtgat ctttatggat gagggtaaaa ttgtcgaaga
    37141 ctcgccgaaa gatgcgttct tcgatgatcc gaaatcggac cgcgcaaaag acttcctcgc
    37201 gaaaatcctg cattaatcac tctggcgcgc atacccaatt gcgcgccatc tcacgcttta
    37261 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    37321 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    37381 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    37441 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt cttcgtatgc tgaccttgct
    37501 caatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    37561 tatcgcggac aatgtgcacg gcgaaagcgg tctcgacggc ccggcattac cggaaccggc
    37621 gttcgcaccg caaaactgta cggcggtaga gctaatggcg aaaacgctgc gtgaaagtgc
    37681 ggaacctgtc accattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    37741 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    37801 tggtaactgg acgccagcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    37861 tgtcttccag tcagggatcc cggtggtgat ggccggtctg gatgttactc ataaagcaca
    37921 aatccacgtt gaagacaccg agcgtttccg cgcgattggt aaccctgttt caaccattgt
    37981 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    38041 cgcaccactg catgacccat gcaccatcgc ctggctgttg aaaccggagt tatttacctc
    38101 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat acccagggta tgacggttgt
    38161 tgattattat tatctgacag gcaataaacc gaatgccacc gtaatggtcg atgttgatcg
    38221 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    38281 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctctt tttctttcaa
    38341 gatataaatg caatggataa tgcagaactc gccattggta tcgatctcgg tactaccaat
    38401 agtttaattg ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa
    38461 tatttaacac catccataat tagcatggat gaaaataatc atattttagt cggaaaaccg
    38521 gctgtatcac ggcgtacttc gcatccggat aaaacggcag cgttatttaa acgtgcaatg
    38581 ggcagtaata ccaactggcg gttaggcagc gacacattta acgcgccaga actgtcctct
    38641 ttggtattac gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc gattaaagat
    38701 gtggtgatct ctgttccggc ttatttcagc gatgaacaac gcaagcatac ccgtttagca
    38761 gcggagttag ccggtttaaa tgcggtacgc ttaattaatg aacccacagc agctgcgatg
    38821 gcgtatggcc tgcataccca acaaaatacc cgttcgctgg tgtttgatct cggtggtggc
    38881 acgtttgacg ttacggtgct tgagtacgcc acgccggtga ttgaagttca cgcctccgct
    38941 ggcgacaact ttcttggtgg cgaagatttt acccatatgc tggtggatga agttttaaag
    39001 cgcgcggatg tcgccaggac cacgcttaac gagagtgaac tggcagcctt gtacgcctgt
    39061 gtggaagcgg caaaatgtag caatcaatcg ccaatgcaca ttcgctggca gtaccaggat
    39121 gaagcacggg aatgcgattt ttacgagaat gaactggaag atttgtggtt gccgctgctc
    39181 aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg cgcgtctgaa gccgagtcaa
    39241 atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc
    39301 gtgcgtctgt ttggcaaatt accgtatcaa agttacgatc cgagcaccat tgtcgcgctg
    39361 ggcgcagcaa tccaggcagc ctgccgctta cgcagtgaag atattgaaga agtgatcctc
    39421 actgatattt gcccttactc gttgggcgtt gaagttaacc gtcagggcgt atccggcatt
    39481 ttctcgccga ttattgaacg aaacaccaca gtacgggtgt cgcgtgtaga aacttactcg
    39541 accatgcacc cggagcagga ttcaattacg gttaacgtct atcagggaga aaaccacaaa
    39601 gttaaaaata acattctggt ggaatccttc gatgtgccgt tgaagaaaac cggggcttat
    39661 cagtcgattg atattcgctt tagttatgat atcaacgggt tgcttgaagt tgacgtgctt
    39721 ctggaagacg gcagcgttaa gtccagagtg attaaccaca gcccggtaac attgagcgcg
    39781 cagcagattg aagagagtcg gacgcggtta tccgcattga aaatttatcc gcgcgatatg
    39841 ctcatcaatc gtacctttaa agccaaactg gaagagttgt gggcgcgggc gctgggtgac
    39901 gagcgagaag agattggccg ggtgatcacc gattttgatg cggcgttgca gtcaaacgat
    39961 atggcccgcg tggatgaagt tcggcggcgg gcgagcgatt atttagccat tgagatccca
    40021 taatcacggc tgccggatgc ggcgtaaatg ctgcatccgg catcggcgca aaaaacacat
    40081 cgtcaactaa acaacatcgc gattttgccg agagcataac ctataaccag caccgttaaa
    40141 ctgattttga taatgctggt aatgctctgt ggtttttcac cctcatcatt tttttcttca
    40201 tggttttcag gtgttggctc atcgacatct tcatcctccg gctcgagttt tactttcgtc
    40261 aacagcatcc gggtgataat ggcctctaat ggatgagcat ctccgccttc cagccttgta
    40321 ttaagacggg ccagcatctc ttgtttcgga aacgcttcaa gtactacctg ttccgctacc
    40381 cgatacaaat ccagtaacgg tgaatcttgc tctgtctcgc taccgactct cgatttccac
    40441 cacgcccatg ctttgcgttt gaagaatagc gactcaataa acgattgacc taacggcgat
    40501 aattttccgc tatgtaaaat ctccgcccat aacagccgcg tccgggcgct ctcactagga
    40561 acatatttca atggtttacc ttgcgcatct accatgctcc gcgcttcgat aaccatcact
    40621 aataacgcga aataatccgg gcaatgacgc ttgctccact gcaacaacaa attatcagct
    40681 tgtgtagatg ggtaactttc ccagtacgcg cagagatcgg caagcaggct ttccccctcg
    40741 ccgcagtaca aacgttgtgc acacaagtaa tatcgggcat cttcgctttc tggttcttcc
    40801 atttgccact gttgagctac aggaatgagt tccgccatac ctgtttgcac cgagctgtac
    40861 cagcgcaaca gtttgcgatg caacttcgca tcatcaggaa tgaaccacgg accttccatc
    40921 gccaaaaacg cagtgacata gtcaggatgg taacggcagc aacgttccag cgcgaagtaa
    40981 tactcaaccg tctggttctg caacgcaatg ggatattcca gaaggctaag gaaatcgaaa
    41041 caatcaccag cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc cattccttcg
    41101 ctgtcattaa acgaatgcca gttcaaacgc tctgccagca aagaagcaca attcaatgaa
    41161 agatatcgcg cttctatagc gattgcacac agcggccagc gtaattgatc gacatcctcc
    41221 atgttccagg tatttaattg ctgaataaat ttctgccagg cggagggctg aaaacgatca
    41281 ctttctgaat ccagtaatgt cctgaatgca cgtagtattt catgttcagc ggtggcatct
    41341 ttttcttcat catctgcttc ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag
    41401 gcctggcgta atgctttaaa cccctgcgga tcggtttcgg gatgacacga cggtaagcga
    41461 gcaagataag cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc
    41521 caacatgttt tcataggtcg ctcacatcct taagcgttac cgggcattca ggctggacag
    41581 ttaatggctg ccagggaagc gtggcataga aacggtcgct tcccgctgcc agctcttcga
    41641 acatccgact cccggcgcta tcgtatttgt acagggttag cgcctcgcgt agcgaagcgt
    41701 tgtcttcgca ctgagaagat tgccagtaga gacgccctag tgaataggca gaaaaatagt
    41761 gcatccagct gtgatagtaa tggtgagccc gtacatagac gcgcgattgc agccataaac
    41821 tctcttcttc cgtcagccag ttgttgagca caccgacccg tgacagaaac cccattcgaa
    41881 cataatccca ggctttgatt cccccttccc cacagcacat agcggtttgg gcaacgaatt
    41941 gggtataaat caaccctctc tccgagccac cagcaataaa tgctttccac tcttcagggg
    42001 aagagcgatg ccatgtcaga tataagccag ctaaatcatt ggcatgacca tcatcagtca
    42061 tctgataaat agtctgaatt aaatcatcac gattatttat tccccaggat gatgagagat
    42121 cgaattcttt tatcccagta tacatttttg gcgagtcatg cgcgggtttg caaccctgat
    42181 taagtatttc catgggcgca gaaagcgcaa aaagaagata ttgttcttcc ttattcattt
    42241 gttaataatc ccttaagtgc attaactgac tactttttta ttattgatga gtataaactt
    42301 gacgctttca aaataaaaag aaaatcgaag cattcacaca tgaataaaaa attaatgtat
    42361 atattcgcaa tttttatagt tgcagcaatt acctgtatta gccaacccaa gaaaacgacg
    42421 ttgcgtgata aagccatggc gaattatgcc tttgattatt taagctcacc gggcagtctt
    42481 ccattcacca cggcagccac ggagctttcc gcgattcatg gtcactcaac gtcgcaatat
    42541 cgccttggag aattttatct tcatggtagc gacggtaaac cactggatta tacacaggcg
    42601 agatactggt atgagcaatc agcggaacag gaaaatccac gcgcgcaaag taaactgggg
    42661 tggatctacc tcaaaggtct gggggtcaag cccgacaccc gtaaagcaat tctctggtat
    42721 aaggaagcag ctgaacaagg gtatgctcat gctcaatata ctttaggttt aatctacaga
    42781 aatggctcag gtattaatgt taaccattat gaatctcaaa aatggttaaa actggccgcc
    42841 aaacaacatt acaaaaacgc ggaaagatta cttgccgggc ttcccgcaca ttaacgtcga
    42901 ctgtcacccg aacagatgga gtattttccc tatcaaaccg gcaaagataa aaatgtagaa
    42961 aataattttg atgacgccca ggcagcccgg cccaccatcg tcaacgggga tcgactgcgg
    43021 ttgcggctcc ggttcaacag tctgcttctc ggtaaaattc gccgcctgaa ttaataatcg
    43081 acgaataatt aatgcttcca gtggacaacg atcttctgca tcaagacggc ttacaatcgg
    43141 cattaatgat tcttctgtag gttggctggc ctgcactaat tgtttggcaa ctagatataa
    43201 tggccataag gtatgttctg tatcacgcgt gagtgagatc cgcggcttac gtcctttgtg
    43261 caaaaccatc tcgaccagtg aacgccccag agcagagagt ttttcatcgc tgagtatttc
    43321 gtataaatga aaacgcgtac gggcgctgtc accagggaca taaagtagcg gttttccctg
    43381 atcgttaacc agttcacgag cttcaatcat catcaccact aacgggtaat aatcgacccg
    43441 atgctgacgg caccattgca atattaaagc atccgcctgg gtggagggat actcgcgcca
    43501 gtagtcacaa agttctgcca gcaagctttc gccttctcca caatagacgc gttgtgcgta
    43561 ttcataataa cgcgcatctt cattatcaga ttcagcttcc cgccacgacc gggcgtaacc
    43621 ctggagttcg ggaattccca ggcgacctgc gctataccag cggagtaaat ttctgtgcat
    43681 ttttgcatca tcggggataa tcactgcgcg atgttgcgct aaatacaatg tcagccattc
    43741 tggatgcgat gaccaaatcc gagcgaacat ttggtaaaaa tcgatggtgt cattttgcac
    43801 ggcaacgggc agatgcagaa tggtctggaa gttaaaaaca ttccccttcg caatggcata
    43861 taaaaaggat tccagttctt cttcgtctat ttccccggta ttttcctcct gtaaccaccg
    43921 caatctttct gctaataaca ccacgcactc gaaggataaa tgggcagtgt tcatggctat
    43981 tgtgcacagc gaccagcgta attcatcaat ctcatccatc gagcaataat ttaattgctg
    44041 aatgaatcgc tgccaggcgg agggcagaaa acgttcacta tcagaggcaa gtaacgcacg
    44101 aaaggcgagc agaatttcat gttctgctac ctcatattct tctggttgcc aaacagattt
    44161 agcaggcgac tgcgcaatcc ggagcgcttc ctcatacgcc tgacgaagtt gtttaaaacc
    44221 ctgcggatcg gtttctggat gaaaggacgg taacagcgcc agataagcgc ggcggataat
    44281 atcgacgtca gtcgtttcct ctatgttgag gatcttccag caatttttca taggtcgctc
    44341 atatccttaa gcgtttccgg gcatgtgggg tagtcagcca gtggttgcca gggtaaggtc
    44401 gcgtaatatg catcgtcact ggcaaataat tgataaaaca tatcattgcc ggaatcgtcg
    44461 tattctttac gcgctaagaa ttcgcgtaat aaaggcagat gatcatcttc gggagactgc
    44521 caatattgcc gaccaaaggt ataaccggca aagtattgcc gccagttact ataataacgt
    44581 agagcccgta gatgaatacg cgactggatc catagacttt cttcttcact caaccagtta
    44641 ttaagtacgc ccattcggct taaaaaaccc atccggacat aatcccaggc tttgattccc
    44701 ccttcgccac agcattcggc ggtgctggcg acaaattgcg cataagcctg accttgctca
    44761 tttaattcag caagatagtc acgccattca catggcgaat aacgaaacca gcggtgataa
    44821 aaccctgcca accgagcggc atgaccgtta tcgatcattc ggtgaattgt gcgcagcagg
    44881 tcatcacggt catcaattcc ccatgaggaa gaaaggtcaa aaaagcatgt acctaaatac
    44941 attttgggtg atgaatgcga aggtaaacag cattcattgc gtatttccat cggagtggaa
    45001 agtgtaaaca atattttttg c
//
LOCUS       DAZFZC010000033        43366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFZC010000033 DAZFZC010000000
VERSION     DAZFZC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.033"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            164..835
                     /locus_tag="ACHKXP_002062"
     CDS             164..835
                     /locus_tag="ACHKXP_002062"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO7647071.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            845..2041
                     /gene="yaiP"
                     /locus_tag="ACHKXP_002063"
     CDS             845..2041
                     /gene="yaiP"
                     /locus_tag="ACHKXP_002063"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7647072.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQNKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2019..2600
                     /locus_tag="ACHKXP_002064"
     CDS             2019..2600
                     /locus_tag="ACHKXP_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7647073.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2602..3375
                     /gene="yaiO"
                     /locus_tag="ACHKXP_002065"
     CDS             2602..3375
                     /gene="yaiO"
                     /locus_tag="ACHKXP_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO7647074.1"
                     /translation="MIKRTLLAAAILSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3561..3836
                     /gene="frmR"
                     /locus_tag="ACHKXP_002066"
     CDS             3561..3836
                     /gene="frmR"
                     /locus_tag="ACHKXP_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO7647075.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3871..4980
                     /gene="frmA"
                     /locus_tag="ACHKXP_002067"
     CDS             3871..4980
                     /gene="frmA"
                     /locus_tag="ACHKXP_002067"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO7647076.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5073..5906
                     /gene="fghA"
                     /locus_tag="ACHKXP_002068"
     CDS             5073..5906
                     /gene="fghA"
                     /locus_tag="ACHKXP_002068"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7647077.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6130..6669)
                     /gene="yaiL"
                     /locus_tag="ACHKXP_002069"
     CDS             complement(6130..6669)
                     /gene="yaiL"
                     /locus_tag="ACHKXP_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO7647078.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6771..7982)
                     /gene="mhpT"
                     /locus_tag="ACHKXP_002070"
     CDS             complement(6771..7982)
                     /gene="mhpT"
                     /locus_tag="ACHKXP_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter"
                     /protein_id="HFO7647079.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQSAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRIQPCADA"
     gene            complement(8146..8315)
                     /locus_tag="ACHKXP_002071"
                     /pseudo
     CDS             complement(8146..8315)
                     /locus_tag="ACHKXP_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8560..9573)
                     /gene="mhpE"
                     /locus_tag="ACHKXP_002072"
     CDS             complement(8560..9573)
                     /gene="mhpE"
                     /locus_tag="ACHKXP_002072"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO7647080.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHVQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9570..10520)
                     /gene="mhpF"
                     /locus_tag="ACHKXP_002073"
     CDS             complement(9570..10520)
                     /gene="mhpF"
                     /locus_tag="ACHKXP_002073"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO7647081.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10517..11326)
                     /gene="mhpD"
                     /locus_tag="ACHKXP_002074"
     CDS             complement(10517..11326)
                     /gene="mhpD"
                     /locus_tag="ACHKXP_002074"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO7647082.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRTGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11336..12202)
                     /gene="mhpC"
                     /locus_tag="ACHKXP_002075"
     CDS             complement(11336..12202)
                     /gene="mhpC"
                     /locus_tag="ACHKXP_002075"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO7647083.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12220..13164)
                     /gene="mhpB"
                     /locus_tag="ACHKXP_002076"
     CDS             complement(12220..13164)
                     /gene="mhpB"
                     /locus_tag="ACHKXP_002076"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO7647084.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(13166..14830)
                     /gene="mhpA"
                     /locus_tag="ACHKXP_002077"
     CDS             complement(13166..14830)
                     /gene="mhpA"
                     /locus_tag="ACHKXP_002077"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO7647085.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LNTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            15021..15854
                     /gene="mhpR"
                     /locus_tag="ACHKXP_002078"
     CDS             15021..15854
                     /gene="mhpR"
                     /locus_tag="ACHKXP_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO7647086.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15931..17013
                     /gene="lacI"
                     /locus_tag="ACHKXP_002079"
     CDS             15931..17013
                     /gene="lacI"
                     /locus_tag="ACHKXP_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO7647087.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            17136..20210
                     /gene="lacZ"
                     /locus_tag="ACHKXP_002080"
     CDS             17136..20210
                     /gene="lacZ"
                     /locus_tag="ACHKXP_002080"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO7647088.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPMNVAPQGKQVIE
                     LPELPRLESTGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASNTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20262..21515
                     /gene="lacY"
                     /locus_tag="ACHKXP_002081"
     CDS             20262..21515
                     /gene="lacY"
                     /locus_tag="ACHKXP_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO7647089.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21581..22191
                     /gene="lacA"
                     /locus_tag="ACHKXP_002082"
                     /pseudo
     CDS             21581..22191
                     /gene="lacA"
                     /locus_tag="ACHKXP_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
     gene            complement(22294..23475)
                     /gene="cynX"
                     /locus_tag="ACHKXP_002083"
     CDS             complement(22294..23475)
                     /gene="cynX"
                     /locus_tag="ACHKXP_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO7647090.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALVGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23481..23951)
                     /gene="cynS"
                     /locus_tag="ACHKXP_002084"
     CDS             complement(23481..23951)
                     /gene="cynS"
                     /locus_tag="ACHKXP_002084"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO7647091.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23982..24641)
                     /gene="cynT"
                     /locus_tag="ACHKXP_002085"
     CDS             complement(23982..24641)
                     /gene="cynT"
                     /locus_tag="ACHKXP_002085"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO7647092.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24750..25649
                     /gene="cynR"
                     /locus_tag="ACHKXP_002086"
     CDS             24750..25649
                     /gene="cynR"
                     /locus_tag="ACHKXP_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO7647093.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPSITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(25986..27269)
                     /gene="codA"
                     /locus_tag="ACHKXP_002087"
     CDS             complement(25986..27269)
                     /gene="codA"
                     /locus_tag="ACHKXP_002087"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO7647094.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLLDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(27259..28518)
                     /gene="codB"
                     /locus_tag="ACHKXP_002088"
     CDS             complement(27259..28518)
                     /gene="codB"
                     /locus_tag="ACHKXP_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO7647095.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(28754..30640)
                     /gene="prpE"
                     /locus_tag="ACHKXP_002089"
     CDS             complement(28754..30640)
                     /gene="prpE"
                     /locus_tag="ACHKXP_002089"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO7647096.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(30680..32131)
                     /gene="prpD"
                     /locus_tag="ACHKXP_002090"
     CDS             complement(30680..32131)
                     /gene="prpD"
                     /locus_tag="ACHKXP_002090"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO7647097.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(32165..33334)
                     /gene="prpC"
                     /locus_tag="ACHKXP_002091"
     CDS             complement(32165..33334)
                     /gene="prpC"
                     /locus_tag="ACHKXP_002091"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO7647098.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAKHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(33774..34664)
                     /gene="prpB"
                     /locus_tag="ACHKXP_002092"
     CDS             complement(33774..34664)
                     /gene="prpB"
                     /locus_tag="ACHKXP_002092"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO7647099.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDNLFARSQVK"
     gene            34903..36489
                     /gene="prpR"
                     /locus_tag="ACHKXP_002093"
     CDS             34903..36489
                     /gene="prpR"
                     /locus_tag="ACHKXP_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO7647100.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(36587..36862)
                     /gene="yahO"
                     /locus_tag="ACHKXP_002094"
     CDS             complement(36587..36862)
                     /gene="yahO"
                     /locus_tag="ACHKXP_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO7647101.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            37009..37680
                     /gene="yahN"
                     /locus_tag="ACHKXP_002095"
     CDS             37009..37680
                     /gene="yahN"
                     /locus_tag="ACHKXP_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO7647102.1"
                     /translation="MMQLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(37697..37942)
                     /gene="yahM"
                     /locus_tag="ACHKXP_002096"
     CDS             complement(37697..37942)
                     /gene="yahM"
                     /locus_tag="ACHKXP_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO7647103.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGWGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAVIRQIRLIT"
     gene            complement(38355..39170)
                     /gene="yahL"
                     /locus_tag="ACHKXP_002097"
     CDS             complement(38355..39170)
                     /gene="yahL"
                     /locus_tag="ACHKXP_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO7647104.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(39413..40462)
                     /gene="yahK"
                     /locus_tag="ACHKXP_002098"
     CDS             complement(39413..40462)
                     /gene="yahK"
                     /locus_tag="ACHKXP_002098"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO7647105.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHIGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(40549..40623)
                     /locus_tag="ACHKXP_002099"
                     /pseudo
     CDS             complement(40549..40623)
                     /locus_tag="ACHKXP_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40839..42221)
                     /gene="yahJ"
                     /locus_tag="ACHKXP_002100"
     CDS             complement(40839..42221)
                     /gene="yahJ"
                     /locus_tag="ACHKXP_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO7647106.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKLHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGLDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELANRMVVQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(42231..43181)
                     /gene="yahI"
                     /locus_tag="ACHKXP_002101"
     CDS             complement(42231..43181)
                     /gene="yahI"
                     /locus_tag="ACHKXP_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO7647107.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIIITPECLPAALRGETGTHIIKT"
     gene            complement(43257..>43366)
                     /gene="yahH"
                     /locus_tag="ACHKXP_002102"
     CDS             complement(43257..>43366)
                     /gene="yahH"
                     /locus_tag="ACHKXP_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YahH"
                     /protein_id="HFO7647108.1"
                     /translation="ARESVGLISVAHQAFVTIAGCGVNALSGLRVAREL"
BASE COUNT        10161 a        11790 c        11206 g        10209 t
ORIGIN      
        1 tgaattgcat agaaacatta cccagtcgaa cccgcgggtc tgaaagccct tgcttttcag
       61 ttattagcaa catttataaa ttaacatctg gctgcgccag agattacgtc tctgagtgca
      121 tcgttgtgct tgtagtataa taaataaaat ggagtgtcat taagtggata aggttttaga
      181 ttcagccctc ctttcttcgg caaataaaag aaagggtatc cttgctattg gcgcacatcc
      241 tgacgatata gaattaggct gtggcgcatc gcttgctcgt cttgcgcaaa aaggaattta
      301 tatcgcagcc gtggtgatga ctaccggcaa ctctggcaca gatggaataa tcgatcgcca
      361 tgaagaatcg cgcaacgccc taaagatatt agggtgccac caaactattc atcttaattt
      421 tgctgacacc cgcgctcatt tacagctcaa tgatatgatt tccgccctgg aagacatcat
      481 taaaaatcaa attccttctg atgttgaaat catgcgggta tataccatgc atgatgccga
      541 ccgccatcag gatcatcttg ctgtttatca agcttcaatg gttgcctgcc gaactattcc
      601 gcaaattctc ggctacgaaa ccccaagtac ctggctttca tttatgcctc aggtttttga
      661 atccgttaaa gaagaatatt tcacagtcaa acttgctgca ttaaaaaaac ataaaagcca
      721 ggaacgacgt gattatatgc gtcatgatcg cctgcgtgca gttgcacaat ttcgcgggca
      781 acaggtcaat agcgatctgg gtgaaggctt tgtaattcat aaaatgattc tttgagcaaa
      841 ttgcatgaaa acctggatat ttatctgtat gtccatagca atgttgctat ggtttttaag
      901 tacgctaaga cgtaaaccca gtcaaaacaa aggctgtatt gacgccatta tacctgcgta
      961 taacgaaggc ccgtgtctgg cgcagtcact ggataatcta ctgcgtaacc cttatttttg
     1021 ccgggtaatt tgcgttaacg acggctccac ggacaatacc gaagcggtca tggcggaagt
     1081 caaacgcaaa tggggcgacc gctttgttgc cgtcacgcaa aaaaataccg gtaaaggtgg
     1141 tgcgctgatg aatggcctca attacgccac ctgcgaccag gtttttttaa gtgatgccga
     1201 cacctatgtt ccgcccgatc aagacggaat gggctatatg ctggcagaaa ttgagcgcgg
     1261 tgctgatgcc gtaggcggca ttccctctac tgcgttgaaa ggcgcggggc tgttaccgca
     1321 catccgcgcg accgtaaagt tgccgatgat tgttatgaag cgcacgctac agcagctcct
     1381 gggcggcgca ccgtttatta tcagcggtgc ctgcgggatg ttccgtactg atgtattgcg
     1441 taagttcggt ttctcggatc gtactaaagt cgaagacctt gatctcacct ggacattggt
     1501 ggcaaacggc taccgtattc ggcaggcgaa tcgctgcatc gtatatccac aggaatgcaa
     1561 cagcccgcgt gaggagtggc gtcgttggcg gcgttggatt gtgggctacg cggtctgtat
     1621 gcgcctgcat aaaaggcttt tatttagccg tttcggtatc ttcagtatat ttcctatgct
     1681 gttggttgtg ctttatggcg ttgggattta tctcactacc tggtttaatg aattcatcac
     1741 caccgggccg catggtgtgg tgttggcaat gtttccgctt atctgggtcg gcgtagtttg
     1801 tgttattggt gcttttagcg cctggtttca tcgttgctgg ttgttggtgc ctttagcgcc
     1861 gctttccgtt gtgtatgtat tattagctta tgccatctgg attatttatg gacttattgc
     1921 cttttttact ggacgcgaac ctcagcgcga caaacccacc cgctattccg cactggtgga
     1981 agcgtcaacc gcttattccc aaccttctgt cacaggaact gaaaaactat ctgaagctta
     2041 atgttaaaga gaaaaatatt cagattgcag accaggtaat tattgatgaa actgcaggtg
     2101 aagttgttat cggcgcgaat acccgtattt gtcatggtgc cgttattcag ggtccggtag
     2161 tgattggcgc aaactgcctg ataggtaatt atgcgtttat tcgtcctggc acaataatca
     2221 gcaatggtgt aaaaattggt tttgccaccg aaattaaaaa tgcggttatt gaagcggaag
     2281 caacgattgg tccgcaatgc tttattgccg actcggtagt tgcaaaccag gcatatttgg
     2341 gcgcacaagt acgtaccagt aatcatcgtc tggatgaaca gcccgtgtct gttcgaactc
     2401 cagagggaat tatcgctacc ggatgcgata aattaggttg ttatatcggg cagcgttcac
     2461 gccttggcgt acaagttatt attttgcctg ggcgaattat ttccccgaac acgcaacttg
     2521 gtccgcgcgt gattgtagaa cgtaatttac ctaccggaac ttattcactc cgacaagaac
     2581 ttatccgtac aggagattaa aatgataaaa cggacgttat tagcagcggc cattttaagc
     2641 gcattgcccg cttatgccgg gttaacttcc attaccgcgg gctacgattt taccgattat
     2701 tctggcgatc atggcaaccg taatttagcg tatgctgaac tggtggcgaa agttgaaaac
     2761 gcaacgctgc tttttaatct ttcccagggg cgtcgtgatt atgaaactga acatttcaat
     2821 gccactcgcg gtcagggtgc ggtctggtat aaatggaata actggctgac aacccgaacg
     2881 ggtattgcct ttgcggataa tacgccggtc tttgcccgcc aggattttcg tcaggatatt
     2941 aacctggccc tgttgccaaa aacgcttttc acgaccggtt atcgctacac taaatattac
     3001 gatgatgtcg aagtcgatgc ctggcaaggc ggcgtatcac tctatactgg cccggtgatc
     3061 accagctacc gctataccca ttatgactcc agcgatgcag gtggtagtta tagcaatatg
     3121 atttccgtgc gtctgaatga cccgcgcggc actggttata cgcaactatg gctaagccgc
     3181 ggaacaggtg cttacaccta tgactggacg ccagaaacac gctacggcag catgaagagc
     3241 gtcagtctgc aacgtattca accgctgact gagcaactta atctcgggct gacggcaggt
     3301 aaagtgtggt acgacacccc aaccgatgat tataacggtc tgcaacttgc agcccatctg
     3361 acctggaaat tctgattcct tctgccgccc gctatccggg gcggccttcc ctgccgatta
     3421 gcccccccct ttcctctttg ttttccgacc acattcaccg gataaatttt attctccagt
     3481 attatatact ataggggggt atgcattgac atatagcata cccccctata gtatattgcg
     3541 tgcagataat gaggtgcgaa atgcccagta ctccggaaga gaagaaaaag gtccttactc
     3601 gagttcgtcg tattcgaggg cagattgatg ctctggaacg gtcgctggag ggtgatgccg
     3661 aatgccgtgc catactccaa cagatcgctg ccgttcgggg cgcggctaat gggctgatgg
     3721 cagaagtgct tgaaagccat atccgggaaa cgtttgaccg aaatgactgc tacagccgcg
     3781 aagtcagcca atccgttgac gacactattg aactggttcg agcctatctt aaatagctga
     3841 atctattacc atattgagga agagcgagag atgaaatcac gtgctgccgt tgcatttgct
     3901 cccggtaaac cgctggaaat cgttgaaatt gacgttgcac caccgaaaaa aggtgaagtg
     3961 ctgattaaag tcacccatac cggcgtttgc cataccgacg catttaccct ctccggggat
     4021 gacccggaag gtgtattccc ggtggttctc ggtcacgaag gggccggcgt tgtggttgaa
     4081 gtcggtgaag gcgtaaccag cgtcaaacct ggcgaccatg tgatcccgct ttacaccgcg
     4141 gagtgcggcg agtgtgagtt ctgtcgttct ggcaaaacta acctctgtgt tgcggttcgc
     4201 gaaacccagg gtaaaggctt gatgccagac ggcaccaccc gtttttctta caacgggcag
     4261 ccgctttatc actacatggg atgctcaaca ttcagtgaat acaccgtggt cgcggaagtg
     4321 tctctggcca aaattaatcc agaagcaaac catgaacacg tctgcctgct gggctgtggc
     4381 gtgaccaccg gtattggcgc ggtgcacaac acagctaaag tccagccagg tgattctgtt
     4441 gccgtgtttg gtcttggcgc gattggtctg gcagtggttc agggcgcgcg tcaggcgaaa
     4501 gcgggacgga ttatcgctat cgataccaac ccgaagaaat tcgatctggc tcgtcgcttc
     4561 ggtgctaccg actgcattaa cccgaatgac tacgacaaac cgattaaaga tgtcctgctg
     4621 gatatcaaca aatggggtat cgaccatacc tttgaatgca tcggtaacgt caacgtgatg
     4681 cgtgcggcgc tggaaagtgc acaccgcggc tggggtcagt cggtgatcat cggggtggca
     4741 ggttctggtc aggaaatctc cacccgtcca ttccagttgg tcactggtcg cgtatggaaa
     4801 ggttccgcgt ttggcggcgt gaaaggtcgt tcccagttac cgggtatggt tgaagatgcg
     4861 atgaaaggtg atatcgatct ggaaccgttt gtcacgcata ccatgagcct tgatgaaatt
     4921 aatgacgcct tcgacctgat gcatgaaggc aaatccattc gaaccgtaat tcgttactga
     4981 tttcccgcag gtataccccg tccacttcag acggggttct taatactctc cctgggcagc
     5041 cgtccggggg attaaccgtg agataatgac tgatggaact cattgaaaaa catgccagct
     5101 ttggcggctg gcaaaatgtg tatcggcatt attcccaatc actgaaatgt gaaatgaatg
     5161 tcggcgtcta tctcccacca aaagccgcga atgaaaaatt gccggtgctg tactggcttt
     5221 caggcctgac ctgcaacgag cagaatttca ttactaaatc ggggatgcag cgttacgcgg
     5281 ctgagcacaa cattattgtt gttgcgccgg acaccagtcc gcgaggcagt catgtcgcag
     5341 atgctgaccg ttacgatctc gggcaaggtg ccgggtttta cctgaacgcg acgcaagcgc
     5401 cgtggaatga acattacaaa atgtatgact atatccgcaa cgagctgccg gatttagtga
     5461 tgcatcattt tccggcaacg gccaaaaagt ctatctctgg tcattctatg ggcgggctgg
     5521 gcgcgctggt gctggcgtta cgtaacccag atgaatatgt cagcgtctcg gcgttttcgc
     5581 ccattgtctc cccatcgcaa gtgccgtggg gacagcaagc ctttgctgca tatcttgctg
     5641 aaaataaaga tgcctggttg gattacgacc cggtgagtct tatttcacaa ggtcaacgcg
     5701 ttgcggaaat catggttgat caggggttga gtgatgattt ttacgcagaa cagctgcgga
     5761 ctccaaatct tgaaaagatc tgccaggaga tgaatatcaa gacgttaatc cgttatcacg
     5821 agggttatga tcacagctat tattttgtct ccagttttat tggcgagcat attgcctacc
     5881 acgccaataa actgaatatg cgttgataat agcacgactg ccggatgcgg cgtgaacgcc
     5941 ttatccggcc tacacttcgc ccgtaagccg taggcctgat aagacgcgca aagcgtcgca
     6001 tcaggcatgg cgcacgactg ccggatgcgg cgtgaacgcc ttatccggtc tacacttcgc
     6061 ccataaaccg taggcctgat aagacgcgca tagcgtcgca tcaggcatct gcgcacgact
     6121 tatctatgtt taccacatca aatcatcagg cactttgaag tcggcatacg ggtcgtcttc
     6181 atcctgctct tcggcgctga gcgcgctgtg caacactata ctgctggcat cgcgctgagc
     6241 aattttatcg gcaacgctgg cgggaataat ggcgtattca ccttcgctat tgttatcgac
     6301 caacaagcgg gcaatcgcca gacgaccatt aatcaactgc gcctgtgtga gcttatcgac
     6361 aaaaatcttc ttaatcagat tgccgtcagt gaagttaaaa ccaatatcgc cattggcaat
     6421 ggtgattcgg ttcatttcaa taagctgctt cacctgagct ttatattctt tcgccaacgc
     6481 cgcttgtttt tgctgttcgc taagctgttt atcacgctca agctgtgcct ttttattttc
     6541 ttctaccgcc gcccgagctt cacgcgcctg aacgcgcgat tttttcgccg ttctctccac
     6601 cttcgccgct tttttgctgg tgactaatcc tgctttgagc aactgctctt gtaaggtaag
     6661 ttttgccatc tttgtttcca aacccgctga ataattttta gcgagtatac ctgtaaacgc
     6721 cgtaggtgta ttggggtgaa gtccctttcg cccctgccta ataaggcacg tcaggcatcg
     6781 gcgcacggct gtattcgtga tctccggctc atcaaaataa acaccgccaa cccagcaaca
     6841 agaatacccg gtgcagaagc ggccattacg ccgaccgtgc cagtgcctaa tgccagcatt
     6901 ttcccggcca gtaacggacc gctcatagcc cccagacgcc ctaccgccac ggctgttccc
     6961 acacctgttg cgcggatctg cgaactgtaa aacaacggtg ccagggcata caaaacgctt
     7021 tgcccacctg tcgcaaacaa ccccgcgaca aatcccgcca gcaacatacc gttaaatgac
     7081 gacaccgttc caagcgccag cagcgaagct aacatgccgc tataaatcag tagcgacatg
     7141 gttactggac gcagcttatc catcaatgcg cccaacatta acgtcccgct tgccgccccc
     7201 atttgcaggg caaacatcac ccctgccgcc tgcgatggct ggaatccttg ctccaccaaa
     7261 agtagcggta gccagttgat caacatgtag accaccagca gagtgaagaa ataacacaac
     7321 cacagcagca gcgtcgcggt tgccgtttct ggcgcaaata aggcacgcag tggtggcgca
     7381 gactgttttt cgccagcgaa aaccgccgac tccggcagcc agcgcattaa tagcggcacc
     7441 agaatcaacg gcaccacacc acctacccaa aacaccgttt gccatgctaa gtttgccccc
     7501 gcgaaaccca gtgtcgccgc cagcgccgcg ccaatgggaa caccgcaata catcaggctc
     7561 actgccgtcc cacgaaaacg tggacccgcg gcttcagacg tcagggcgat aagattcggc
     7621 aacgccgccc ccagcccgac accggtcatc agccgcgcaa agaccagtga ggggaaatcc
     7681 caggcaatcg ccgttgccag tgagaacaaa ccaaacagcg caactgagcc aatcaaaatg
     7741 cgcttgcgac cataacggtc cgccagcatt ccgccaacca acgcgccggg tagcaaaccg
     7801 agtattccgg cgctaaatat ccagcccatt tgcattttat cgagtgcgaa agcctgggcg
     7861 atgccacccg ccgcaatgcc agccgcctga agatccagcc cttccatcag agcgaccaaa
     7921 aaacaaagcc cgatggtcag catcaggcgg gatgaagatg atgaaggggt acgagtcgac
     7981 ataagatgaa cctcgttgcg agtgcaggtg aatcgcgact gatacattgc cagccgcgta
     8041 atcagaacta tttaatgact gcctacgggc gaaatgtagg ccggacaaga cgtttatcgc
     8101 atccggcagt cgtgtgcaga tgcctgatgc gacgctaacg cgtcttatca ggcctacaaa
     8161 ttcccgcgcc atccgtaggc cggataaggc gtttacgccg catccggcag tcgtgcgcag
     8221 atgcctgatg cgacgctaac gcgtcttatc aggcctacaa attctcgcgc cacccgtagg
     8281 ccggataagg cgttcacgcc gcatccggca gtcgtgcgca gatgcctgat gcgacgctaa
     8341 cgcgtcttat caggcctaca aattcccgcg ccatccgtag gccggataag gcgttcacgc
     8401 cgcatccggc agtcgtgcgc agatgcctga tgcgacgcta acgcgtctta tcagacctac
     8461 aaattctcac gccatccgta ggccggataa ggcgttcacg ccgcatccgg cagtcgtgct
     8521 gggatgggca tacaaaatgc acgttctcgg cagtcatcat tatttgttgt tgcgcagatc
     8581 cagcgccacg tcaacgatca tatcctcctg gccgccaacc atccggcgtt tgcccagctc
     8641 aacgagaata tccaccgcac ttaagccata acgcgccgcc gccgtttcac agtgacgcag
     8701 gaagctcgag taaacaccag cgtatcccag cgccagcgtt tcgcgatcga ctcgtaccgg
     8761 tcgatcctgc aacggacgca ccaggtcgtc ggcggcatcc attaacgcat agagatcggt
     8821 cccatgctgc cagcccagtt tatccgcggc ggcaataaac acttccagcg gtgcgttacc
     8881 tgcgcccgcg cccattcccg cgaggctggc gtcgattcgg tcgcagccct cttccaccgc
     8941 cgcgatagag ttcgccacgc caagactcag gttatggtga gcgtgcatgc cagtttgcgt
     9001 ttctggtttc agctctgctt tcagggcgcg gaaacggtca cggatatcgc tcatgttcat
     9061 cgcaccgcca gaatccacca cataaataca ggtcgcaccg tagccttcca tcagctttgc
     9121 ctgcttggcg agattctccg gcgtggtcat atggctcatc atcagaaaac caacggtgtc
     9181 cattccgagc tcgcgggcat actgaacatg ctgggcggaa acatcagctt cggtacagtg
     9241 cgttgccaca cgaaccaccc gcgcgccagc ctgccaggca tttttcagat cgtgaatagt
     9301 gccgattcct ggcagcaaca acgtcgcgat tttggcgtgc ttcaccacat ccgccgccgc
     9361 ttcaatccat tcaaggtcgc tatgtgcgcc gaaaccatag ttaaagctgg aaccttgcaa
     9421 accgtcgccg tgggccactt caatcgaatc cacgcgggca tcgtccagtg ctttggcaat
     9481 ctggcgaacg ttttccagcg aatactgatg acgaatggcg tgcataccgt cacgcaatgt
     9541 gacgtccgag atataaagtt ttttaccgtt catgccgctt ctcctgcctt gcgcgccagt
     9601 gactgggcca ttttttccgc tgtcgccagc gcactggaag tcataatgtc gaggttgccc
     9661 gcataggcag gcagataatg cgctgcgcct tcgacttcca gccagaccgc tgttttcagt
     9721 ccggagaatt gccccacgcc cggtaaattg accggtttat cctgcgggat aacttcaaac
     9781 tgcacgcgct gtttcaggcg ataacccggt acgtaagcct gcaccgcctc agccatttca
     9841 ttgattgagg cttcgatatc atcttgtgaa gcttcgtcgc tcaatacata caccgtgtca
     9901 cgcatcatca acggtggctc tgctgggtta agcacaataa tcgccttccc ttttgccgcg
     9961 ccgcccacca cttcaatggc tcgggaagtg gtttccgtaa attcatcgat attggcacgc
    10021 gtgccaggtc cggcagattt actggcgata gaagcgataa tttcggcgta atgaacacgc
    10081 gccacgcgtg aaactgccgc caccattgga atggtggcct ggccgccgca ggtgaccatg
    10141 ttgacgttca gttgatcgac gttcgcctcg aggttaacca ccggcacgca gtaagggccg
    10201 atggcagcag gcgtcaggtc aattaagcga atatccggtt tcgcttcgcg taaagcggca
    10261 tcgtttttca catgagcacc ggcgctggtc gcatcaaata caatgtcgat atcagcaaat
    10321 tcaggcatgt tcatcagtcc gatcacccct tcatgggtgg tggcgacgcc catacgtctg
    10381 gcgcgcgcca gaccgtcgga ctgaggatca atgccaacca tcaccgccat ctccagatgc
    10441 tgaccgtgac gcaaaatttt aatcatcaga tcggtaccaa tgttgccaga accgataatg
    10501 gcgactttac gcttactcat gacagacttc cttttggggc tgcgcttgaa aatgtcgcag
    10561 caactgaacc tatgccttca atatgggctt caaaacgatc gcccgcattc accgccacca
    10621 tcggacctaa tgccccggta agaatgatat ctccggtgcg cagcggttca cccagactgg
    10681 ccattttgcg tgccagccag acggccgcat taagcggatg tcccaggcat tcgctgccgc
    10741 gcccgctaga aacctcttcg ttattacgcg tcatcttcat ggcgcagttt ttcaggtcta
    10801 accccgccgg acgttgcgcc ggaccgccga tgacatacac cccacaggag gcgttatctg
    10861 ccacggtatc gacaaactga atcgaccagt cgcgaatgcg gctccccacc acttccagcg
    10921 ccggaagtac ccattcaatg gcgttataca attcgtcgaa ggtgatatcg gttgcgggca
    10981 aatcgcggtt caacaccagt gcgatctccg cttcaatgcg gggttgcaga acacgggaaa
    11041 aaggaatgat ttcgttatcg ccataacaca tgtcggcaaa taacgtccca aaatccggtt
    11101 gatcaacgcc cagttgttgt tgcactttcg gatgtgtcag gcccacttta cgccctacca
    11161 cgcgacgccc ctgcgcaacg tcatgttgca catttatgtg ctgaatggcg taagccgctt
    11221 cagcgttatc gataccaatc agatcgcgca gcggtgcaat cgcttcgccc tgctctgcgg
    11281 cgcggcgtaa atccgccgcc agttgctcaa gagtatgctt cgtcatgacc attccttaag
    11341 ggcgtgcgag gaaattcagc accagttgat tgaaagcgtc ggcatgttcc cactgcgccc
    11401 agtgaccaca gtcgcggaag atatgcagtt cagaaccggc aatgccggac agcagacgca
    11461 gacccgcatc catcggcaca aagcggtcgt tgcgccccca gacaatcagg gtttgcgctt
    11521 tgatttccgc cagacgtggg ccaaaatccg ggaactgttt cggattggct tccaggctct
    11581 taacaaagtt ttccaggtga tcgcggcgcg acagcatatt attcaggcgc gcttcaaaca
    11641 gggcgtcggt caaatcgctg gtatcaaaaa cgaagatatc catcatcagc ttcaggtttt
    11701 cgatagtcgg ctgacgataa agctgattca gtcgcttaat accttcggtt ggcatcggcg
    11761 taaacaaact catgccgccc gtaccgccgc ccatcagcac cagtttgccg acgcgctccg
    11821 gccatttaag ggtgaacgcc acagaactat ggccccccat cgagttgccc agcaggtgga
    11881 ttttggcgat atccagttga tccaccacgc ttttcaggat tcgtgcatta agatccgatc
    11941 gcgaaccact attaacgatc gaatcgctct tgccccaacc cggacaatcc agcaggatca
    12001 cccgatagcc cgcctctacc agcggatcga tattgcggct gaagttcgcc cagccagtag
    12061 cacccgggcc ggaaccatgc agcaggacaa cggtttcgtc gccttgtccg cagtcattaa
    12121 aatggatgcg cagcgtttta cccgcttctt ctacattcag aaaacggctg gtggcggctt
    12181 cggtttgtgg ctgataactc atcatcttct cctgcatatt cagttctctg ttctggcgct
    12241 taacgagcca aatccggcaa tccactccgg gattgggcgg taataacgcc cttcgctacg
    12301 ccagttgcca aacgcagaaa tagcggcaaa agcggcgacc caggttttga tttcatgtgt
    12361 cgactttccg gcaatggcgg aaagctcttc gttactgacg gcatccagtt cctgtatgcg
    12421 tccctgctcc agcaaagtca tgaactggtt atcccaaatc gggttgagcg gatgcagcgt
    12481 tctctgatcc tcaacaaact tctcagcggc gctaatcacc cgttgctgac gcaattcgcg
    12541 ctcactggcg ggtaaatctt tcccgctccc caacagacgg tcgcgcatat gggcatcggc
    12601 tttcgccagt tcgggcaccg gcggctgatg ggaaagccca ccggaaccca ggaacagcac
    12661 gcgtttattg agagtgctgg tgaaacgtcc aatggcttca cccaacatgc gggtacgctg
    12721 gaaaccgggc agcggcgtgg cgacaccgtt gatgaacaca ggcagaactg gcaccttatc
    12781 cagcccaccg agcaggaact ccagcggctg ggcgaacccg tggtccacct gcatacagta
    12841 agaaacggca agatcgatcc cgctcttcat gacggcatgc gcacaggcct ccgccagctc
    12901 cacaggcacg ggcagctctc ctgccgcact gccgaaatca ccaattgccg tcgctccaac
    12961 gcctaaacag aacggtggca tcacgtcata gaaaaagccg ttgtagtgat ctggcgcaaa
    13021 cagcaccacc agttcagggg agaatgccgc aatacgctcg cgggcgctgg caatcacgcc
    13081 attgacctca tcgagtacct cttgtgccgg gtcgacatac cccaccagcg gcgagtggga
    13141 aagacaatga agataagcgt gcatatcagg ctaccttttc gacagaaacg tcggcatcag
    13201 ggcgggtcag cgtcatcacc gacgccagtt tattcagggt cttgcccagg gtttgcggaa
    13261 tggcggtggc ggcaacaaag cgatccgggc gcatcaccac cagcgaagca ttgtgttgtg
    13321 cgaaccagct acgcagacga ccttgcgtat cgcccacgcg tagcacgccg tcgtggttat
    13381 cctgtgccgt atgaatttgc acttccggca ccacctgaat aaagcgtgtg cccaacgcgc
    13441 gccactgctg gatttgctcg tcgctcatcc cccacagtgg attgcatccc cagccaatta
    13501 ccgcgaagtt cgcgccgatc gcgttatcga gcagcgtcac gtcgccgttt tccagcgtga
    13561 ctttcggctg aataaacatc ttgccgaccg gagagtgctt cgcttcgccc tcacgtacca
    13621 gcgcaccgcc gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag tagcgtttta
    13681 ctggcggcag ataattcagc agccaggaaa cgccgtcacg taacgtaccc tgccagcgtt
    13741 tcggtggagc cagcacgttg cccgccgtca cggacaggtc aatcatcgct ttggcgtgat
    13801 cgcgacgttc ttgttgatag gtattgagca gcgcatcgcg ggctttcccc tggataacca
    13861 acgccagttt ccatgcgagg ttaaaggcgt cgcgcatacc actgttatag ccctgcccct
    13921 gccacaccgg catgatgtgc gcggcatcgc ccgccagcag tacgcgatca atacggaaac
    13981 gttgcgccag tcgcgcgttg tgggtgtaga cacgctggcg aatcaattca acattgtccg
    14041 gattaggcag cactttgctt aacagcttgc gcatattttg cggctcacgc agctgttctt
    14101 cggtttctcc tggcatcacc ataaattcaa agcgacgtac cgcatgaggt agcgcggcag
    14161 aaacatacgg gcgcaccgga tcgcaacaca aatagatatg cggcgtactt aacggatcgt
    14221 tggcgatatc taccacaatc cactgatttg gcgcagtttt accttcaaac ggcacattca
    14281 gggtgcgacg gacaaaactt gccccaccat cacaggccac cagccactgg gctttgacta
    14341 tttcccgctg cccttctgcc gttttcaggt gcaaggtcac ttcgtcatct tgctgactga
    14401 aggcctccag ctcgcgggaa aacaagcagc gcacattcgg aaaacgcgac accccttcca
    14461 gcatcaccgc atcgacctgc ggctgaataa aggcgttacg gcgcggccag ccaaattcat
    14521 cggtcattgg ctgaatatca gcaaaacagc ggcctttcgg ggtgagaaaa cgcatcgcgt
    14581 gccacggcgt agtgtgcggc agaacatcat cgaccaggcc gaccgactgc atggtgcgca
    14641 gcgcctcgtc atcaatacca atcgcacgcg ggtagtcgat caacttatcg agtttctcca
    14701 ccaccagcac gtcaatgccc atctggccga gatagttcgc catcatcagc ccaaccgggc
    14761 cggcaccagc gatcgccacc tgaacgctat ggttaacagc aggctggatg tcagggtgtt
    14821 gtattgccat ttcagtatct cacgactcgg acaaaatgcc gttgcgcgca cagtacagcg
    14881 caacttattt tgttaaaaac atgtaaatga ttttttattg tgcgctcagt ataggaaggg
    14941 tgttttcggc tacaatcaaa acatgctcga atgtgcacca ggtgcacaac gttgttttaa
    15001 ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa accgtgcgcg
    15061 gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc ggtgccagcg
    15121 tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga ctgctggaga
    15181 cgctgcagga agagggatat gtccgccgta gcccctccga tgacagtttt cgtctgacca
    15241 tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct gcactggcgg
    15301 ccccactgct gggcgatctg ttgcgcgaag tggtatggcc gacagatgtg tccacgctgg
    15361 atgttgatgc aatggtggta cgcgaaacca cccaccgttt cagccgctta tcttttcacc
    15421 gggcaatggt cgggcgacgt ttgccgcttc tgaaaaccgc ctcgggcctg acctggctgg
    15481 ccttttgccc ggaacaagaa cgcaaggaat taatcgagat gttagcctcc cgccccggtg
    15541 atgactatca actggcacga gaaccgttaa agctgcaagc cattctggcg cgcgcgcgca
    15601 aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatc gcctctatcg
    15661 ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg tatatggcga
    15721 gcgcaatgac cattgaacag gccgcggaaa agcatcttcc ggcgctacaa cgggtagcaa
    15781 aacagatcga agaaggggtt gaatcgcagg ctattctggt agccggaagg cgaagcggcg
    15841 tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc
    15901 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata cgatgtcgca
    15961 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
    16021 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
    16081 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
    16141 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
    16201 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
    16261 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
    16321 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
    16381 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
    16441 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
    16501 tctgtctcgg cgcgtctgcg tctggcaggc tggcataaat atctcactcg caatcaaatt
    16561 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
    16621 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
    16681 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
    16741 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
    16801 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
    16861 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
    16921 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    16981 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    17041 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtgaaat
    17101 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
    17161 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    17221 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    17281 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
    17341 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
    17401 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
    17461 cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta
    17521 atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact
    17581 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc
    17641 cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg
    17701 tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca
    17761 ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg
    17821 ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg
    17881 gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg
    17941 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
    18001 gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc
    18061 tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct
    18121 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
    18181 cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
    18241 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
    18301 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
    18361 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
    18421 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
    18481 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggtgctaatc
    18541 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
    18601 gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
    18661 aagaccagcc cttcccggcg gtgccgaaat ggtccatcaa aaaatggctt tcgttgcctg
    18721 gagaactgcg cccactgatc ctttgcgaat atgcccacgc aatgggtaac agtcttggcg
    18781 gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct
    18841 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa tggcaatccg tggtcggctt
    18901 acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgcatgaac ggtctggtct
    18961 ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc
    19021 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
    19081 ataacgagct cctgcattgg atggtggcgc tggatggcaa gccgctggca agcggtgaag
    19141 tacctatgaa tgtcgctcca caaggtaaac aggtaattga attgcctgaa ctaccgcgac
    19201 tggagagcac cgggcaactc tggctcacag tacgcgtagt acaaccgaac gcgaccgcat
    19261 ggtcagaagc cggacacatc agcgcctggc aacagtggcg actggcggaa aacctcagcg
    19321 tgacactccc ctccgcgccc cacgccatcc cgcaactgac caccagcgaa acggattttt
    19381 gcatcgagct ggataataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
    19441 tgtggattgg cgatgaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
    19501 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg
    19561 aacgctggaa ggcggcgggc cattaccagg ccgaagcggc gttgttgcag tgcacggcag
    19621 atacacttgc cgacgcggtg ctgattacca ctgtccacgc atggcagcat cagggaaaaa
    19681 ccttatttat tagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
    19741 ttgatgttga agtagcgagc aatacgccac atccggcgcg gattggcctg acctgccagc
    19801 tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
    19861 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
    19921 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
    19981 cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagccaa caacaactga
    20041 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
    20101 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc
    20161 agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
    20221 gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca
    20281 caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact
    20341 tcccgttttt cccaatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta
    20401 ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt
    20461 ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt
    20521 ttgcgccgtt ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat
    20581 cgattgttgg tggtatttat ctaggctttt gttttaacgc cggtgcgcca gcagtagagg
    20641 catttatcga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg
    20701 gctgtgttgg ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc atcaataatc
    20761 agtttgtttt ctggctgggt tctggctgtg cactcatcct cgccgtttta ctctttttcg
    20821 ccaaaacgga tgcgccctct tctgccacgg ttgccaatgc ggtaggtgcc aaccattcgg
    20881 catttagcct taagctggcg ctggaactgt tcagacagcc aaaactgtgg tttttgtcac
    20941 tgtatgttat tggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct
    21001 ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt atttggctac gtaacgacaa
    21061 tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc actgatcatt aatcgcatcg
    21121 gtgggaaaaa cgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat
    21181 cgttcgccac ctcagcgctg gaagtagtta ttctgaaaac gctgcatatg tttgaagtac
    21241 cgttcctgct ggtaggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag
    21301 cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg
    21361 tactggcggg caatatgtat gaaagcatcg gcttccaggg cgcttatctg gtgctgggtc
    21421 tggtggcgct gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggcccgcttt
    21481 ctctactgcg tcgtcaggtg aatgaagtcg cttaagcaat caatgtcgga tgcggcgcga
    21541 gcgccttatc cgaccaacat atcaggacgg aatgatcgca atgaacatgt cgatgacaga
    21601 aagaataaaa gcaggcaagc tatttaccga tatgtgcgaa ggcttacctg aaaaaagact
    21661 tcgtgggaaa acgttaatgt atgagtttaa tcactcgcat ccatcagaag ttgaaaaaag
    21721 ggaaagcctg cttaaagaaa tgtttgccac ggtaggggaa aacgcctgga tagaaccacc
    21781 tgtctatttc tcttacggtt ccaacatcca tataggccgc aatttttatg caaatttcaa
    21841 tttaaccatt gtcgatgact acacggtaac aatcggtgat aacgtactga ttgcacccaa
    21901 cgttactctt tccgttacgg gacaccctgt acaccatgaa ttgagaaaaa acggcgagat
    21961 gtactctttc cgataacgat tggcaataac gtctggatcg gaagtcatgt ggttattaat
    22021 ccaggcgtca ccatcgggga taattctgtt attggcgcgg gtagtatcgt aacaaaagac
    22081 attccaccaa acgtcgtggc ggctggcgtt ccttgtcggg ttattcgcga aataaacgac
    22141 cgggataagc aatattattt caaagattat aaagttgaat cgtcagttta aattagcaaa
    22201 attgcctgat gcgcttcgct tatcaggcct acaagttcag tgatctacat tggccgcatc
    22261 cggcatgaac aaagcgcagg aacaagcgtc gcatcatgcc tctttgaccc acagctgcgg
    22321 aaaacgtgct ggtgcaaaac gcagggttat gatcatcagc ccaacgacgc acaacgcatg
    22381 aaatgcccag tccatcaggt aattgccgct gatactacgc agcacgccag aaaaccacgg
    22441 ggcaagcccg gcgatgataa aaccgatccc ctgcataaac gccaccagct tgccagcaat
    22501 agccggttgc gcagagtgat cgagcgccag cagcaaacag agcggaaacg cgccgcccag
    22561 acctaaccca cacaccatcg cccacaagac cggcaattgc agcggcaacc agataaagcc
    22621 gcagaacccc accagttgta acaccagcgc cagcattaac agtttgcgcc gatcctgatg
    22681 gcgagccata gcaggcatca gcaaagctcc tgcggcttgc ccaagcgtca tcaatgccag
    22741 caaggaaccg ctgtactgcg cgctggcacc aatctcaata tagaaggcgg gtaaccaggc
    22801 aatcaggctg gcgtaaccac cgttaatcag accgaagtaa acacccagcg tccacgcgcg
    22861 aggagtgaaa accacgcgaa ccggagtggt tgttgtcttg tgggaagagg cgacctcgcg
    22921 ggtgctttgc caccaccagg caaagagcgc aacaacggca ggcagcgccc accaggcgag
    22981 tgtttgatac caagtttcgc tatgttgaac taaccagggt gtcagggcgg caccaagccc
    23041 accgccgccc atcagagccg cggaccacag ccccatcacc agtggcgtgc gttgctgaaa
    23101 ccgccgttta atcaccgaag gcatcaccgc ctgaatgatg ccgatcccca ccccaccgag
    23161 cagtgcgctg ctaagcagca gcgcactttg ggggtaaagc tcacgcatca atgcaccgac
    23221 ggcaatcagc aataaactga tggcgacgct gcgacgttcg ctgacatgct gatgaagcca
    23281 gcttccgacc agcgccagcc cgcccatggt gaccaccggc agagcggtca acagggcagc
    23341 cacgctaaag ctcattccgc ttgcctggcg caattgcggt agcagtggcc cgacggaggt
    23401 gagcagtggt cgcatattaa gaccaatcag caccagaacc agcagcatcg tgaacgtctt
    23461 ttgatggtta cgcatggctg tcagaacggt ttggtcggca gatatttacc atctaaggtg
    23521 atgaccgcac gttcgccacc ttccgggtcc gccactttct taacgtcgag tttgaagtta
    23581 atcgcgctga taatgccatc gccaaacttc tcatgaacca acgctttcag ggttgtgccg
    23641 tacacctgca acatttcata gaaacgatac atcgttgggt cggttggaat acggtcatca
    23701 atgcagccac gcagtggaat catctgcaac agtagaatgg cgtcttcgtc gagatccagc
    23761 ttcgccccta ccaggcgggc ggcgtcggca ggaagcgcct gctgccccag caaagccgcg
    23821 gttacgaagg cttctgccag accggtaccg tcggcaatct cggcaaatga gagatctttt
    23881 ttagctttgc tgagcaaaat ggcatcggca agatcaagac gaatattgcg gttaatttgt
    23941 gactgaatca tggtggaact cctgatggtt taaaaataag gttacgctgc ggtcggttgg
    24001 cgtagcggta tggcacaaac gcgaggatta gcggccagtg gcacaaactg gcgggttgcg
    24061 ccgtcaaaag ctgcgatgct gccgctttca atgtcgtaga cccagccgtg cagggcgatc
    24121 cgcccctctt cgagcgccag gcgcaccgat ggatgagttt gcaaattagc caactgagca
    24181 atgacgtttt cacgtaccat cgccgcagct tttgacggta aatcggaatg cgggcgcgcc
    24241 tcattaacga cgcgggctga atcggcataa cgcagccagt gggagacggc aggcatatgg
    24301 tccatgcact gacagctggc aatggcggtc atcgcgccac agttggaatg accacaaatc
    24361 acaatgtcag atacccgaag cgcagcgacg gcatactcca ccgaagcaga aacgccaccg
    24421 ggttccggcc cgtaggaagg gacgatattg cccgcgttgc gaataacgaa cagatcgcca
    24481 ggctcacgtt gcgtcaccag ctcagggacc agacggctgt cggagcagga gataaaaagt
    24541 gtgcgcgggc tttgctgtgt cgccagctgt ttaaacaagg cttcccgctt cggaaatgcc
    24601 tcgcgctgga atttaaggaa tccatcaata atctctttca ccgttaacct ctgtctgtct
    24661 ctggaatgag aggcagacta ctcgccgatt gtcataaggt aaaagtctca tttatgatga
    24721 gttccattgg atttacttat aggttgcgaa tgctctctcg acatatcaat tattttcttg
    24781 ccgtggctga acatggcagc ttcacccgtg ccgccagtgc gttgcacgtc tcccagcctg
    24841 cgctttccca gcagattcgc cagttagagg agagtttagg cgtgccgctg tttgaccgta
    24901 gcgggcgaac gattcgtctc actgatgcag gagaagtctg gcgacagtac gccagccggg
    24961 cgttacagga actgggggcg ggtaaacggg cgattcatga tgttgccgat ctgacgcgag
    25021 gatcgctgcg tatcgccgtc acccccacct ttacgagcta ctttatcggc cccttaatgg
    25081 cggatttcta tgcgcgctat cccagcatca cgctccagct acaggaaatg tcgcaggaga
    25141 aaatcgagga tatgctttgc cgcgacgagt tggacgttgg gattgccttc gcgcctgtgc
    25201 attcgccgga gctggaggca attcctttac tgacagaaag tttagcgtta gtcgtggcgc
    25261 aacatcatcc gctggccgtc catgaacagg tggcgttgag tcgcttgcat gatgaaaaac
    25321 tggtcctgct cagcgcggaa tttgccacca gagagcaaat tgaccactac tgcgagaaag
    25381 cggggctaca tccacaggtg gtcattgagg cgaactcaat tagcgcggtt ctggagctga
    25441 ttcgccgcac ttccctttcc acattgttac cagcagcgat tgccacacaa catgacgggc
    25501 ttaaagctat ttctcttgcc ccgccactac tggagagaac ggcggttttg ttgcggcgga
    25561 aaaatagctg gcagacagcc gccgcgaagg catttttgca catggcgttg gataaatgcg
    25621 cggttgttgg cggaaatgaa tcacggtagc cgctgacaca ggtctgcggc tggaattgcc
    25681 tgatgcgcta cgcttatcag gcctacgagt tcagtgctct acgtaggccg gataaggcgt
    25741 tcacgccgca tccggcataa actacagctg gcagactgcc gctgcgaagg catttttgca
    25801 catggcgttg gaggaatgcg cggttgttgg cgggaatgaa tcacggtagc cgctgacaca
    25861 gatcagcggc tggaattgcc tgatgcgcta cgcttatcag gcctacgagt tcagtgctct
    25921 acgtaggccg gataaggcgt tcacgccgca tccggcataa actaagctcg ctgtaaccca
    25981 gtcgttcaac gtttgtaatc gatggcttct ggctgctcca gatatacggt ggtttgtgcc
    26041 ggttgtgtgc tggcaatcac cttgccgcca cgtaccgaat aacgtaccgg aacctgacgg
    26101 cgcagcgcat caaacccatt ttcagccggc aggataatca ggtttgcgct gtttccggcg
    26161 gcaatgccgt aatcctgcaa attcaacgtt ctggcactgt ggtgggtgat taaattcagg
    26221 ccatcgttaa tctgcccgta gcccatcaac tggcaaacat gcagccccat atgcagcact
    26281 tgcagcatat tcgccgttcc cagcggatac cacggatcga agacatcatc gtgaccaaag
    26341 cagacgttaa tgccggactc cagcatctct ttaacgcgcg tgatgccgcg acgttttgga
    26401 tacgtatcga atcgtccttg cagatgaata ttgaccagcg ggttggcgac aaagttaata
    26461 ccggacattt tcagcaagcg gaacagacgt gaggtatacg ccccgttata ggagtgcatt
    26521 gccgtggtgt ggctggcggt gactcgcgcg cccatgcctt cacggtgcgc cagggcagca
    26581 acggtttcga caaagcgcga ctgctcgtca tcgatctcat cacagtgaac gtcgatgaga
    26641 cggtcgtatt tttgcgccag ggcgaaggtt ttatgcagcg actccacgcc gtattcacgg
    26701 gtaaattcaa aatgcggaat cgcccccact acatctgccc ctaagcgtaa cgcctcttcc
    26761 agcaacgctt caccgttggg atacgacaaa atcccttcct gagggaaggc gacaatttgc
    26821 agatcaatcc acggcgcgac ttcctgcttc acttccagca ttgctttcag cgcagttagc
    26881 gttgcatccg aaacatcgac atgggtacgc acatgctgaa tgccgttggc aatctgccat
    26941 ttcagcgttt gccaggcgcg ttgtttcaca tcgtcatggg ttaataacgc tttgcgctcg
    27001 gcccagcgtt caatgccttc aaacagcgtg ccggactgat tccagttcgg ttgtccggcg
    27061 gtttgcgtgg tgtccaggtg aatatgcggc tccacaaacg gcggtataac taaaccttgt
    27121 tcggcatcca ggctgttttc agttatgggc atcacgccgg attgcgcatc aatggcgctg
    27181 atttttccgt ccagcagatg aatctgccac agcccctctt tgcctggtaa ccgggcgtta
    27241 ataattgttt gtaaagcgtt attcgacact gttagcctcc acatgcgtca ttgctgctgt
    27301 cgttttacga ttcaaaatcg ggttaaggat cagatagctc agcgcgccac ctaataccgc
    27361 gttgaccgga acaattcccg gtaaccagtg gcctgcggca atccccaagg cgaccgccag
    27421 aatcgccacc caattgacac tcatcatacg cgtggtcgca aagtgctcat agcggcgacg
    27481 gttcatcaga tagtcggcga tgatcacgcc acctaccgga ggaatagctg ccgaaaggaa
    27541 ggtcaaccag ccgacaaaat tgttatacag ccataatgcg cagaccgtac cgataatacc
    27601 gttgatcacc gaaagcgttt tgctcgacat accggtaatg ttggcgaaac ctaaacccga
    27661 cgcatagagt gcgttatcgt tggtggtcca gatattcagc ccgagcacca caatcgcagg
    27721 cagcagcagt ccctgagcaa tcatcacatc agagatatcc gccatcccca gtgccgcagc
    27781 gcccgctgca ccgaaaataa acatcaacga gttgccgagg aaaaaggcca ccatcgccac
    27841 cagcaccgcc agtttggcat tgcgaccaaa ccggacaaag tcagcggtga gcgtgcccgc
    27901 actgataaat gaccccacaa ccagcgccag cgcgacattg aaatctaacg gttgtgcggg
    27961 aacgaccgct tttaatgcgt ccaggccgcc catgccgtta acggccagcc acacggaata
    28021 accgccaagg caggcaatag ccggaaccgc aatcaccgaa agaactgtca gcgccgaaat
    28081 gccaaagaag acggtgacgg tcatcagtaa accggaaacg gcaatcagca aattaatatc
    28141 cagcccggtt gccttgccca ccggaatagc aaacatcgcc acgccaacgc caaaccagcc
    28201 aacctgagtt ccgcccagta gcagtgaagg cagccatgag cctttaacac caaaagagaa
    28261 gcgagcaaga agatgagtgg tcaggccggt ttttgcgcca atgtaaccaa gaaatgaagt
    28321 gtaaataccg aggagaagat taccgatgag aactgcgagg aagaaatcat gatagctaag
    28381 accggttccg agagtgccgc cggtccacat actggcggaa aagaaggtta atcccagcat
    28441 gacgaacgtc aatgccaata cccctttccg cgccgactgc gggactggcc cctggctaaa
    28501 gttgttatct tgcgacacga aattccccct ttttgctgtt tgaaacccaa aaaatccgcc
    28561 gcattctatt catctcaaga taaaaagcaa tcgttttcgt ggggaaatat attttttata
    28621 tgaatgagtg caaaaaggtg ggggaaaaat tatcggtgta aaaacagtgg gtaggcctga
    28681 taagacgcgt cagcgtcgca tcaggcatcg gcacacagtg ccggatgcac cgcgcgacgc
    28741 atccgacaat aacctactct tccatcgcct ggcggatctg atccaacgac gcaggatcat
    28801 caatggtcgt cagatctcca ggatcgcgtc cttcgcaaat cgcctggatc gtgcggcgca
    28861 gcatttttcc ggatcgcgtt tttggcaatt gcgagacaaa ccagacgtgc gccgggcggc
    28921 caaagttgcc aatctggctg tccaccagcg ccataatcgc cttctcttgc gagtgcgcca
    28981 catcacgatc ttccagactg tcgctctctt tcggaatgac aaacgccacc gccacctgcc
    29041 ctttcagcgc atctttcacc ccaaccaccg ccacttcggc aacgcccgga tggctggaga
    29101 tactctcttc aatctcgcgc gtccccagcc gatgcccggc aacgttaatt acatcgtcag
    29161 tgcgcccgag aataaagtga taaccgtcag cgtcacggat gccccagtca aaggtggcgt
    29221 acaccgggcg ggaaaacagc gaccagtaag tcttcacaaa gcggccgtcg tcgccccaga
    29281 tggtctgaat acaccccggc ggcagcggcc cttccaccac cagcatccct ttctcgttga
    29341 cgccacacgg ttcgccggtg acttcattaa gcaactgcac gttatagcca tacatcggca
    29401 caccggggct tcccagacgc gtcggcctgt cgtccagacc gcgagcaatc gccataatcg
    29461 gccagccgga ttcggtctgc cagtagttgt cgatgaccgg cacatccagc gtattgctca
    29521 cccaactggc ggtcggctcg tccagcggtt ctccagccag atagagcact tccagcgacg
    29581 agagatcgtg tttgcgaatt tcagcggtag ggaatttttt cagcacgcga atggcggtcg
    29641 gcgctgagaa catccggcta acctgatatt tctcgacgat tttccaccac acgccgcagt
    29701 ccggccaggt cggcaatcct tcgtaaacga tagtcgccat ccccgccagc agcggcgcgt
    29761 aaacgatata cgaatgcccc accacccagc cgatatccga tgcgcaaaag aacacgctgc
    29821 ccgctttgcc gccaaaaatg gtgtccatcg aggtcgccag cgccaccgca tatccgccga
    29881 cgtcacgctg cacgccttta ggtttgccgg tcgtgccgga agtgtagaga atgcaggagg
    29941 tttcgttgga ttccagccac gccaccggta cccgcgcgcc gatgtgttga tggcgcaacg
    30001 acgcgaaatc gacatcccgc ccgctgacgc gcgccatttt cgccagcccg cgatccacca
    30061 gcaaaacatg gcgtggctgg tgctgcgcct gacttatcgc atcgtcgagc aattttttat
    30121 agggaatgat tttgccaccg cgcgctccgg catcagccga gacaatcagc accggtttag
    30181 cgtcatcaat tcgcgccgcc acgctgtgcg aggcaaatcc accaaacacc accgagtgaa
    30241 tagcgccaat gcgcgcgcag gccagcagag taatatgcgc ttcggcaatc atcggcatat
    30301 acaccagcac ccgatcgccg cgctgcacac ccaatgaacg caacattgag gccaccgcgt
    30361 tcacttcgtc atgcagctga cgaaaggtaa aggtgcgctc ttcttctgtt tccgaagaga
    30421 cggcaatcag cgccagcgcc tctggctgtt tctccagcca gcggtcgatg gcgttgtggc
    30481 acaagttggt tcggccttca caaaaccaac gggcaaacgg cggattgctg tgatcgagcg
    30541 tttgcgtaaa gggcgtctgc cagtcaatac gccgggcctg ctcggcccag aactgctccg
    30601 gttcgttaat cgaacgctga taaaattcgc taaaagacat aatgctctcc tgttgaactt
    30661 acgccttacc gccgtttact taaatgacgt acaggtcgag atactcattg accggcatct
    30721 gttccaggcg agctctgtcg agagaaacct ccagaatgcg ctgctgttgg cgagtcggga
    30781 actggcgcgc gagattgatt ttgaatttat cgaccagttt cggaatacca tcctgacggc
    30841 ggcgagcatg accaatgggg tactccacca ccacttcttc aaatcgtgtg ccgtcggtga
    30901 actcaagggt aatggcattg gcgatggcgc gtttttccgg gtcgtggtag tcagcggtaa
    30961 atgccggatc ttcaaagcaa ttgatcttct cgcgcagggc gtcaatgcgt ttatcttgcg
    31021 caacgttgtc ctcgtaatct gccgccgtta agcgcccgaa tagcagcggg atcgccacca
    31081 tgtactgaat gcagtgatcg cggtctgccg ggttattgag cggccctttt ttgtcgatga
    31141 tgcgaataca ggcttcgtgg gtgcgaatgg tcactttttc gatatccgcc gccgttttgc
    31201 ctgctgcctg catctgttca tagagcgtca tcgctgcttc aactgccgtc tgggagtgga
    31261 actccgccgg gaaggagatt ttgaacagca cattttccat aacgtaggaa ccgtacgggc
    31321 gctggaagcg gaacgattca cctttaaagg agacgtcgta gaagccccac accggcgcag
    31381 tcagggctga cgggtaaccc atttcgcccg ttttcgccat cagtgccaga cgtaccgcgc
    31441 gggaagtggc atcgcccgcc gcccaggatt tacgcgtgcc ggtgttcggc gcatggcgat
    31501 aagtgcgcag cgactgaccg tccacccacg ccagcgaaac ggcgttgaga atttcctcgc
    31561 gggtcaggcc gagcatttcg gcgaccacgg cggtggaagc cactttcact aacagaacgt
    31621 ggtcgaggcc gacgcggtta aaggagtttt ccagcgcgat gcagccctga atttcatggg
    31681 ctttgatcat tgcggtcagc acctgtttca tggtcaacgg cgctttgccg ctggcgaccg
    31741 cgttgcgcga aagccagtcc gccgttgcca gaatgccgcc gaggttgtcg gaaggatggc
    31801 cccactccgc cgccagccag gtatcgttga aatcgagcca gcggatcatc gcgccgatgt
    31861 taaatgccgc ctggacgggg tcgagctgga actgagttcc ggggacgcgc acgccgttgg
    31921 gtacgacggt gccgggaaca attggcccca gcagtttttt acaggccggg tattcgagag
    31981 cttccagacc gcagccgagc gtgtcgagca ggcagtaatg tgcggtgtcg taggccactt
    32041 tagagctgat ttcgtagttc atgacgtaat cgacgatatc aacgatttca cgatcaaatt
    32101 ccgggcggat gttgttgatt tgagctgaca ttgggtacgt ttccttattg ttattcgtag
    32161 aggtttactg gcgcttatcc agcgcgacaa acgggcggtc ttccggtcca acataattgg
    32221 cggaaggacg gataattttg ttgtcctgac gttgttcgat aatgtgcgcc gcccagccgg
    32281 tgacgcgggc gataacaaac agtggtgtga acatctcggt gggaacgccc atcatgttgt
    32341 aggaaacagc agagaaccag tcgagattgg ggaacatctt tttgctctcc cacatcaccg
    32401 tttccaggcg atcggcgatg ttgtacatct tcagcgagcc gccttcctgc gagagctgct
    32461 tcgccacacg tttaatcacc tggtggcgcg ggtcagcgat ggtgtaaacc ggatgaccaa
    32521 aaccaatgac cacttctttg ttttccacgc gcttgcggat atctgcttcg gcttcgtccg
    32581 gcgtttcgta gcgttgctgg atctccagcg acacttcatt cgccccgccg tgttttggcc
    32641 cgcgcagtgc gccaatcgcg ccaataatcg cggaatacat atcagagccg gtgcccgcaa
    32701 tcacccgact ggtaaaggtg gaggcgttaa actcgtgttc ggcgtacagc accagcgaga
    32761 tatgcatcgc cttttcccag ctttgcgatg gcttttcgcc gtgcagcaga tgcaggaagt
    32821 gaccgccgat ggagtcgtca tcggtttccg gttggatgcg ttcgccgttg tggctgtagt
    32881 gataccaata aaggagaatc gagctaagcg acgccagcag tttgtcggca atatcccgcg
    32941 cgccagagac ggtatgcccc tctttttctg gcagcgtgca gccgagcgcg gaaacaccgg
    33001 tgcgcataac atccatcggg tgcgacgccg ccggtaaggc ttccagcacg gtacgcacgt
    33061 tagccggtaa accgcgcagg gctttcagtt tcgttttgta agcggcgagt tcgtcacggg
    33121 tcggcagttt gccgtggatc agcagatgcg ccacttcttc aaattcgcaa tgtttcgcca
    33181 gatcaagaat atcgtagccg cggtaatgca ggtcattgcc acttttaccc acggtgcaga
    33241 gcgccgtatt gcccgccgga acgccagaaa gtgccacaga ttttttcggt ttaatgacat
    33301 gggtactgtt ttgcaggatc gttgtgtcgc tcatgttgtc ctcgtcattg ttatcatttg
    33361 tagggtcgag attgttgtcg gatgcgacgc tatgcgtctt atccgaccta cagttcagtt
    33421 caccccgtag gcctgataag tcgcgtcagc ggcgcatcag gcatcggttg tcggatgcga
    33481 cgctatgcgt cttatccgac ctacagttca gtgcaccccg taggcctgat aagtcgcgtc
    33541 agcggcgcat caggcatcgg ttgtcggatg cgacgctatg cgtcttatcc gacctacagt
    33601 tcagtgcacc ccgtaggcct gataagtcac gtcagtggcg catcaggcat cggttgtcgg
    33661 atgcgacgcc atccgtctta tccgacctac agttcagtgc acctcgtagg cctgataagt
    33721 cgcgtcagcg acgcatcagg catcggctgt cgaatacaat caacgggcgt tttttatttc
    33781 acctggctac gggcaaacag gttgtcgagc ttctcttcgt actggtagta gttgatgctt
    33841 tcgtacagct cgttgcgggt ctgcatggtg tcgatgacgc ttttctgcgt gccttcctgg
    33901 cgcaggacgt tgtagacatg ttcagcggcg cggttcatgg cgcggaacgc tgaaagtggg
    33961 tacagcgcca ttgcgacatg ggcgctgcgt aattcgtcgg tggtaaacag cggcgtggca
    34021 ccaaattcgg tgatgttggc gaggatcggc acctgcaccg catcggcaaa ctggcggtac
    34081 atggcgagtt cggtaatcgc ctccgggaac aacatctcgg cacccgcttc aacataggcc
    34141 tgcgcacgct cgatcgccgc atccagcccc tctaccgcca gagcatcggt gcgcgccatg
    34201 atcacaaaat caggatcggt tttcgcatcc accgccgcgc ggatccgatc caccatctct
    34261 tctttcgaga cgatcgcttt attcggacga tgaccgcagc gtttcgcacc aacctgatct
    34321 tcaatatgca atcctgccgc accggcttta atcatcgatt tcacggtgcg cgccacgtta
    34381 aaggccgaag aaccaaaacc gatatccgca tccaccagca gcggcagcga acaaacgtcg
    34441 gtgatacggc gaatgtcggt cagcacatca tcaagggtag aaataccgag atcgggcagc
    34501 cccagcgaac ctgccgccac gccaccgcca gaaagataaa ttgcctgata tccggcacgc
    34561 tgcgccaaca gcgcatgatt agcgttgatg gtgccaacaa tctgcaatgg attttcttta
    34621 gtcagtgcag cgcgaaacgc tttacctgga gagtgtagag acatagccca tcctttgtta
    34681 tcaacttgtt atttgcgttg ataaagacaa agcaaggggt gtgccacaat tgccttaata
    34741 aaattaaatg tttaatttca atttgttaga gataaagtca tgaactaatc cgcaaatatg
    34801 cgtttcagtt aacgtttcag gcaatgtttc accgcgtttc attgcaacaa ttatgaaaca
    34861 agactaaacc caatattcgg tttcttaact ttgcggtgcg ctatggcaca tccaccacgg
    34921 cttaatgacg acaaaccggt tatctggacg gtatctgtaa cgcgcctgtt cgagctgttt
    34981 cgcgatatca gcctcgagtt tgatcacctg gcgaacatta cccctattca gcttggcttt
    35041 gaaaaagcag tgacctacat ccgcaagaaa ctggcaaacg aacgctgtga cgccatcatc
    35101 gcggctggct ctaacggcgc gtacctgaaa agccgcctgt cagtgccagt tattttgatt
    35161 aaaccgagcg gctacgatgt gttacaggca ctggcaaaag ccggaaaact cacctcttct
    35221 atcggcgttg tcacctatca ggaaaccatt ccagcgctgg tggcgtttca aaaaaccttt
    35281 aatttgcgcc tcgaccaacg tagctacatt accgaggaag acgcacgcgg gcagattaac
    35341 gagctaaaag ctaacggcac cgaagcggtg gtcggcgcgg ggctgattac cgatttggca
    35401 gaagaagccg gaatgaccgg aatttttatc tattccgccg ccaccgtgcg ccaggcgttc
    35461 agcgatgcgc tggatatgac gcgcatgtcg ttacgccata acactcacga tgccacccgc
    35521 aacgccctgc gtactcgtta cgtgctgggc gatatgctcg gtcaatcacc acagatggaa
    35581 caagtacggc agactatttt gctgtatgcc cgctccagtg cggcggtgtt gattgagggg
    35641 gaaacgggga cgggcaaaga gctggcggcc caggcgattc atcgggaata ttttgcccgc
    35701 cacgatgcgc gacagggcaa aaagtcgcat ccgtttgttg ccgtcaactg cggggcgatt
    35761 gccgaatcgc tgctggaagc agaactgttt ggctatgagg aaggggcgtt taccggctcg
    35821 cgacgcggag gtcgcgccgg gctgttcgaa attgcccacg gcggtacgct gtttctggat
    35881 gagattggcg aaatgccgct acctttgcag acccggctgc tgcgggtgct ggaagaaaaa
    35941 gaggtcaccc gcgtcggcgg gcatcagcct gttccggtag atgtacgggt cattagcgcc
    36001 actcactgca atctggaaga agatatgcag caaggacgtt ttcgccgtga tctgttttat
    36061 cggctgagta ttttgcgtct gcaattgcca ccactgcgcg agcgggtggc ggatattctt
    36121 ccgctggcgg aaagcttttt gaaagtgtct ctggcggcgc tctccgcccc attttctgct
    36181 gcattacgcc aggggttaca ggcaagtgaa actgtgctgc tgcactacga ctggccaggc
    36241 aatattcgtg aactgcgcaa tatgatggaa cgactggcgc tgtttttaag tgtggaaccg
    36301 acgccggatt taacgccgca gtttatgcaa ctgctactgc cggaactggc gcgcgagtcg
    36361 gcgaaaactc ccgctccacg cttactgaca ccacaacagg cactggagaa atttaatggc
    36421 gataaaacag cagcggcgaa ttatttaggc atcagccgga cgacgttctg gcggcggctg
    36481 aaaagctgaa ctgccgaatg cagcgcgaac gctttatccg gcataaattt caatagatga
    36541 tcaccgataa acttacgcat cggagatgtt tacataggat tcagaattac ttcttcttat
    36601 aaatatttgc cgtgccgtgg atcttattgt cagtttgacc ggaggtcagt accaacacat
    36661 cagccccttt ttcatccgct tttttgatca aatcttcttt tgcatctgca gtcgacattt
    36721 cattgctggt tgaaatatca cctatttttt catactgcga ttcaactttt tcaaattccg
    36781 ctttggtcat caattcagcg gcatagacat tggtaacggc taacgctaac gcaccgacta
    36841 acattttaga gattattttc atattacggc tccagagttg ttatcacact atcacccacc
    36901 cacattattg ggttgattaa taattagttc agtcagggca taaacgccag taaccggaaa
    36961 taagacactt tgtgatctct ctcgcacccg attatttttg gtagaaatat gatgcagtta
    37021 gttcacttat ttatggatga aatcactatg gatcctttgc atgccgttta cctgaccgta
    37081 ggactgttcg tgattacttt ttttaatccg ggagccaatc tctttgtggt agtacaaacc
    37141 agcctggctt ccggtcgacg cgcaggggtg ctgaccgggc tgggcgtggc gctgggcgat
    37201 gcattttatt ccgggttggg tttgtttggt cttgcaacgc taattacgca gtgtgaggag
    37261 attttttcgc ttatcagaat cgtcggcggc gcttatctct tatggtttgc gtggtgcagc
    37321 atgcgccgcc agtcaacacc gcaaatgagc acactacaac aaccgattag cgccccctgg
    37381 tatgtctttt ttcgccgcgg attaattacc gatctctcta acccgcaaac cgttttattt
    37441 tttatcagta ttttctcagt aacattaaat gccgaaacac caacatgggc acgtttaatg
    37501 gcctgggcgg ggattgtgct cgcatcaatt atctggcgag tttttcttag tcaggcgttt
    37561 tctttgcccg ctgtgcgtcg tgcttatggg cgtatgcaac gcgttgccag tcgggttatt
    37621 ggtgcaatta ttggtgtatt cgcgctacgc ctgatttacg aaggggtgac gcagcggtga
    37681 gcagataatt tactctctac gtaatcaacc tgatttgccg aatcacggcc atcggcgatg
    37741 atagcccccg taatgcgtgg tccttatttc cgataagttt acttgagtct ttagcttccc
    37801 atttcagccc actctcccct gtgtgggctt ttttcattgg tctgccccat cctgaatagc
    37861 caaacaatgg caatgttcca ctttgcaggg atgataataa ggggatctga ttcagaagag
    37921 ttttgaaaag ttggaccgcc atcgaggcct cgaaccccgt accatacaac aatcctggtt
    37981 gtcgtctgct cttcctgatg agctaatggc ggtttttgat atgagcctta cagcatattt
    38041 tctgtcaggc ttgagtgcga tgcgtaagat acagaaaaga ccccacacaa acatgatgga
    38101 attatgattt ttttcatatg aatgtcaacc aacacattca caaaacatct gcatgagatc
    38161 attttttata caccgcatgg ctgcgaaacc actactggtg cttttttaag cgaagtgtcg
    38221 ccgatcacaa caaaatatcg gcgaaaaaag agatggaatg ccagttggca tacagatata
    38281 cagatgaaat gattacctgc attgcgattc actctcaggt ggaataacaa cttgcaggtg
    38341 gaaccctttc tgaactaaag agtgtagcgg attttaacgt gcttatttac aggataatat
    38401 ctgtcgccta attcctggac tctattagat atccattgat ttaatcgcgc atcatctctg
    38461 tatgttttta cgtccagaac agagagcaat aacatcacca gtgattcagg aaaaacaggg
    38521 tagatatttt ttaagtgtaa taataaccca tgcgcaagcg cctgcttaat gggcgcgtct
    38581 gtctccggaa tagacgacat ttcgcgaagc catttttcca catgcataca ctctttccct
    38641 ttagggaaaa ttctcagcaa aggcatatgg tgttcaagaa gaatacattt gcaatctgtt
    38701 cgcactgaat cgataaactg cactaatttc ttaagcccgg taaagcgtcc agcctgcacg
    38761 tttgctttaa tatcaagaaa cgcatcatcc tggtcatata cccaaactgt ccctttttga
    38821 ttttcaagta tacgaaaaac aaacttaacc gggtgatcag ggtaatccat ccagtcataa
    38881 aatccaggga aatcattaaa ctgcacattt atggtgagtg cgttatcgtc tgaccactca
    38941 cagtctcgta cggcagccat gagatgaatg agtttgtcgt catcgactaa atcatcacga
    39001 caatcataag gataatcata atattgttta atcaaaacct tgaatatatc gacacaggaa
    39061 accagggagc ttttgatatc ctctggtaac tgattgtttt taaccgtatt aagtatgctt
    39121 tgttgcgtat atggcatcaa attattgtgc ggagctttta atgatatcat cgcgatctct
    39181 cttaaataaa aataaacgtc gcagataata aagctctaat taaaccaggc actatcagaa
    39241 atcgctcata actaacgtgt gaaatattgt gtactggagg gagttaattt aacaatttga
    39301 atttacgtta taggttttac tgctactttg atattatttc gctctcctta actaaaaagt
    39361 taagtggaga gcgaagttaa tatgttaaac cacagcgtat ttattaattt tttcagtctg
    39421 ttagtgtgcg attatcgata acaaaacgat atttcacatc accgcgcagc attcgctcat
    39481 aggcttcatt aatttgatcg gcccgaatca tctctatatc agccacaatg ccatgttcgg
    39541 cgcaaaaatc gagcatctcc tgagtttctg gaatgccgcc aatcatagaa ccggctatcg
    39601 cacggcgttt catgatcagg ttgaaaactt ccggcgattt atgcggtgtc gcaggcgcac
    39661 caaccagcgt catggtgcca tcacgcttca gcaaggtggt aaaatcgtcg agattatgtg
    39721 gcgcagctac tgtattcaaa atgaaatcga aactcttcag atgagccgcc atctcatcgg
    39781 cattgcgtga gttaacaact tcatcggccc ccagggcttt tgccgcttcg cgttttgcct
    39841 cagaagtggt aaatgccacc acatgtgccc ccatcgcgtg ggccagctta atcccaatat
    39901 gtcccagacc gccgatgccg accacgccca cttttttacc cggcccggcc tgccagtgac
    39961 gtagcggcga atacgtggtg atccctgcac acaacaaagg agccaccgcc gccagctgct
    40021 cttgcgggtg acgaatacgc agaacatatc gctcatgaac gacgatctgt tgtgagtagc
    40081 cgcccagagt atggcccggt tcgtccggcg tcggcgagtt ataggtgccg gtcatgtgat
    40141 cacagtagtt ttccaacccg tcttcacact cttcgcaatg tttacaactg tcgacaatgc
    40201 agccgacacc gaccagatcg cccggcgcat atttttctac ctgatcacca acggctacca
    40261 cacgccccac aatttcatga cccggcacgc aggggtaaac cgtccccgcc cactcggaac
    40321 ggacctggtg gagatcggaa tggcaaacgc cacagtaagc gatttcgatt ttgacatcat
    40381 tcggtcccgg ttcacgccgg gtgatatcca tcggttcaag tggttgttta gcggaatatg
    40441 caccaacagc tttgatcttc attgtgttta ctcctgatta gctatgtgta tttggcaacg
    40501 cctgatatgt catcgcggca aaacgcgtcc attgaatata gccaatattt aaaccacccg
    40561 atccagcccc aacatgccgc gagcctgaat gaaacagagc agcaacgttc cccataaggc
    40621 gagcgcctgt aagtgtgata tttgctatct gtaaaagact aaaatcggca aaactgatga
    40681 tcttttcacg tagacaaata gttatctaca caacgtcaat aagggcatat tcctcaaaga
    40741 taatgtccat ttctgccgac acactcatat tcagattgcc ccactccctg gggcttaaat
    40801 ctcttactat tcaccgttgc aggcgtttca aatgcgcatc aacctgccac actcccccac
    40861 accagttgcc ctttatggaa cgttgcggtt ctcggcgaga tacgcgccac cgcctcggcg
    40921 gaacaggagg cgtccaccag cacaaagctg gcgtcatcct gcgcttttgg ccatacacgc
    40981 tcgccttttt cattcagcgg caatacatcg ccagtggcta aaaacagcga gcgggagagg
    41041 ttctgttcgt taggacgaat atagagctgc gcgtacagat tggctttttc cagcatgtcg
    41101 cccagaccat aaggcgacca gtggtcgata acgctgtcag tgccagtcat cacttttacg
    41161 cctttgtcgt gcaactgttt gagcggcata tgcagcgtgc caatcggcac cgtcgaggcg
    41221 atagaaattt gttgcaccac catccggttc gccagttcat ctacctgttg ctcgttgagc
    41281 gttgccagcg caaaggcgtg actgatggtc agcttgccct tcagctgtgg cgttttctct
    41341 accgtttcaa ccatataatt gatggctgcc acgcctgccg gagtggtttc gtgcaggtga
    41401 atatcgaggc ctttgtcgta gtccagcgca atctggaaca tggtgtcgag ggatttttcc
    41461 atcgcgccat caacactggt cgggtctagg ccgccgacgt aatgcgcccc cgcctgcatc
    41521 gcttcacgca ttaaaggttc agatttcgac agcagcaaac cgtgctgcgg gaaggcgacg
    41581 atttcacact caaagcccgc ctgacgtcgc gccagcaccg cctgcaaatt ttgcagattt
    41641 ttcaggccgg aaaccggttc gatattgcag tggctgcggg caatggtggt gcctttcgac
    41701 tgcaataaat caatcagttt ttctgcccgc tcctgagtgt acggttgcag ttccggcagc
    41761 attttctgct cgagtttgat catgtcctgg atggtggtgc ctgccggacg attgagcgag
    41821 cgccacggcc cgccgtaaaa ggttttgtcg agatgaatat gcatgtcgcg ggtggtgggc
    41881 agcatcagct taccgccagc gtcatagtgc ggcagcgtgg cgtccggatg cagcttgttc
    41941 tcgcgcaggg cgacaatttt gccgtcctga atctccacgg tctggcgcgc cgtgcgggtc
    42001 tgcaccgcca cgccattttc gtagtcaaaa ccggtttcca gcagcacgtt atcgagatag
    42061 taatgcgggt cagtgatttt catggtgttg ttcgcttcgc agtttagggt gccagctacc
    42121 gccgcatggg cgagcggcac agaggtacca aacagcgcgg cggcggtgac catcttaccg
    42181 ctctggctca gaaattcacg gcggctattg ctttctttca tcttacgtcc ttacgtttta
    42241 ataatatgag tgcccgtttc gccgcgcagc gccgcaggca ggcattccgg cgtgatgata
    42301 atcacttctt tgccgccttg ttctaaaaat gtcaggctgg cgatgatttt tggcaacatg
    42361 ctgccgggcg ggaaatgccc ttcctgcata tagcgggtca tggtggcaat atccacccga
    42421 tcgagcgcct gctgctgcgg tttgccaaag tgaatacaca ctttttcgac gccagtggtg
    42481 atcacaagaa tgtcggcgtg aatttcacgg gccagcagcg cggtagagag atctttgtcg
    42541 ataaccgcgt ccacgctttg gtaatctccc gcgtcagtac gcactaccgg aattccaccg
    42601 ccgcccgcgc caattacgac aaaaccttgt tggatcagcg ctttaatggc aggtgcttcg
    42661 acaatacgtt tcggttccgg cgaggcgacc acgcggcgat agccccgccc ggcatcttca
    42721 acaaaacacc agtcagggtt tgccttttgt aattcgtcac gctggctgtc actaaagaat
    42781 gcgccgatgg gcttggtggg atgggcaaaa cctggatcgt ttttatccac ttccacctga
    42841 gtcaccacgg tgacggcttt cttctcgccg tgacgcgcca gccggttatt cagcgcctgt
    42901 tggatcagat agccgatgcc gccctgcgta tctgccacac agttcgccag cggcgttaag
    42961 ggcagccctt cgcgctcgtg ggcaatctcc gcacggcgta aatccagccc gacctgcggc
    43021 ccgttgccgt gggtcagcac aatgtcgtaa tcggaagcca gcatttccag caccgtatcg
    43081 gcgacggctt tcaccgcctc cgcctgatgc tcaatcgact ggctggcgtt atctttgata
    43141 atgctgttgc caccaatggc aacgaccaca agctctttca tgttgttatg tccgctacag
    43201 agggtgatgg ggttatgacc aacgccagac tgcctgatgc gctacgctta tcaggcctac
    43261 aattctcgcg ccactcgtag gccggataag gcgttcacgc cgcatccggc aatggtgaca
    43321 aatgcctgat gcgctacgct tatcaggcct acagattctc gcgcca
//
LOCUS       DAZFZC010000034        42460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFZC010000034 DAZFZC010000000
VERSION     DAZFZC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.034"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..502
                     /gene="traM"
                     /locus_tag="ACHKXP_002103"
     CDS             119..502
                     /gene="traM"
                     /locus_tag="ACHKXP_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO7647109.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            689..1378
                     /gene="traJ"
                     /locus_tag="ACHKXP_002104"
     CDS             689..1378
                     /gene="traJ"
                     /locus_tag="ACHKXP_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFO7647110.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVSNKDELIILLYNKKFIYYL
                     YEKAMCIINTR"
     gene            1477..1872
                     /gene="traY"
                     /locus_tag="ACHKXP_002105"
     CDS             1477..1872
                     /gene="traY"
                     /locus_tag="ACHKXP_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HFO7647111.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
     gene            1905..2270
                     /gene="traA"
                     /locus_tag="ACHKXP_002106"
     CDS             1905..2270
                     /gene="traA"
                     /locus_tag="ACHKXP_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO7647112.1"
                     /translation="MNAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            2285..2596
                     /gene="traL"
                     /locus_tag="ACHKXP_002107"
     CDS             2285..2596
                     /gene="traL"
                     /locus_tag="ACHKXP_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO7647113.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            2618..3184
                     /gene="traE"
                     /locus_tag="ACHKXP_002108"
     CDS             2618..3184
                     /gene="traE"
                     /locus_tag="ACHKXP_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO7647114.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGMNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            3171..3899
                     /gene="traK"
                     /locus_tag="ACHKXP_002109"
     CDS             3171..3899
                     /gene="traK"
                     /locus_tag="ACHKXP_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO7647115.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            3899..5326
                     /gene="traB"
                     /locus_tag="ACHKXP_002110"
     CDS             3899..5326
                     /gene="traB"
                     /locus_tag="ACHKXP_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO7647116.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            5316..5906
                     /gene="traP"
                     /locus_tag="ACHKXP_002111"
     CDS             5316..5906
                     /gene="traP"
                     /locus_tag="ACHKXP_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO7647117.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMT
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQGICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            5893..6090
                     /locus_tag="ACHKXP_002112"
     CDS             5893..6090
                     /locus_tag="ACHKXP_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO7647118.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCATRIVLPAPEKFG
                     SESLPDNFNMTAVGVMKNSEI"
     gene            6102..6353
                     /gene="trbG"
                     /locus_tag="ACHKXP_002113"
     CDS             6102..6353
                     /gene="trbG"
                     /locus_tag="ACHKXP_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO7647119.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            6350..6865
                     /gene="traV"
                     /locus_tag="ACHKXP_002114"
     CDS             6350..6865
                     /gene="traV"
                     /locus_tag="ACHKXP_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO7647120.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLERSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            7000..7221
                     /gene="traR"
                     /locus_tag="ACHKXP_002115"
     CDS             7000..7221
                     /gene="traR"
                     /locus_tag="ACHKXP_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO7647121.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            7381..10008
                     /gene="traC"
                     /locus_tag="ACHKXP_002116"
     CDS             7381..10008
                     /gene="traC"
                     /locus_tag="ACHKXP_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO7647122.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRESGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            10005..10391
                     /gene="trbI"
                     /locus_tag="ACHKXP_002117"
     CDS             10005..10391
                     /gene="trbI"
                     /locus_tag="ACHKXP_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO7647123.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            10388..11020
                     /gene="traW"
                     /locus_tag="ACHKXP_002118"
     CDS             10388..11020
                     /gene="traW"
                     /locus_tag="ACHKXP_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO7647124.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            11017..12009
                     /gene="traU"
                     /locus_tag="ACHKXP_002119"
     CDS             11017..12009
                     /gene="traU"
                     /locus_tag="ACHKXP_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO7647125.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            12018..12656
                     /gene="trbC"
                     /locus_tag="ACHKXP_002120"
     CDS             12018..12656
                     /gene="trbC"
                     /locus_tag="ACHKXP_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO7647126.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGVSGK"
     gene            12653..14461
                     /gene="traN"
                     /locus_tag="ACHKXP_002121"
     CDS             12653..14461
                     /gene="traN"
                     /locus_tag="ACHKXP_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO7647127.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVNAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYADNPACTLVSHQCTFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            14488..14745
                     /locus_tag="ACHKXP_002122"
     CDS             14488..14745
                     /locus_tag="ACHKXP_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO7647128.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEIL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            14738..15481
                     /gene="traF"
                     /locus_tag="ACHKXP_002123"
     CDS             14738..15481
                     /gene="traF"
                     /locus_tag="ACHKXP_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO7647129.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQVQQRQAIAKLAEHYGIMFFYRGQGPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            15497..15844
                     /gene="trbA"
                     /locus_tag="ACHKXP_002124"
     CDS             15497..15844
                     /gene="trbA"
                     /locus_tag="ACHKXP_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO7647130.1"
                     /translation="MSEDCLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMNQGAAMVVPMVIGSLPNVTSFDGFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
     gene            15963..16247
                     /gene="traQ"
                     /locus_tag="ACHKXP_002125"
     CDS             15963..16247
                     /gene="traQ"
                     /locus_tag="ACHKXP_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO7647131.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGRHVSH"
     gene            16234..16779
                     /gene="trbB"
                     /locus_tag="ACHKXP_002126"
     CDS             16234..16779
                     /gene="trbB"
                     /locus_tag="ACHKXP_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO7647132.1"
                     /translation="MSLIKSLLFTLLLAAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            16709..17071
                     /gene="trbJ"
                     /locus_tag="ACHKXP_002127"
     CDS             16709..17071
                     /gene="trbJ"
                     /locus_tag="ACHKXP_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO7647133.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEQIKGGQE"
     gene            17071..18444
                     /gene="traH"
                     /locus_tag="ACHKXP_002128"
     CDS             17071..18444
                     /gene="traH"
                     /locus_tag="ACHKXP_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO7647134.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            18441..21266
                     /gene="traG"
                     /locus_tag="ACHKXP_002129"
     CDS             18441..21266
                     /gene="traG"
                     /locus_tag="ACHKXP_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO7647135.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQVIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEITNLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTSTRLSGGMY
                     ADAHAEWGVKPKILGVGGGVGIRGGFKGSLDATDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFALYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIHGEENIVDRQYSELKNNHKQEEIQQNNRYNEENKR
                     QKLMPTPSEDALKQMVDDKKERLKGPL"
     gene            21263..21772
                     /gene="traS"
                     /locus_tag="ACHKXP_002130"
     CDS             21263..21772
                     /gene="traS"
                     /locus_tag="ACHKXP_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFO7647136.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            21786..22517
                     /gene="traT"
                     /locus_tag="ACHKXP_002131"
     CDS             21786..22517
                     /gene="traT"
                     /locus_tag="ACHKXP_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO7647137.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            22770..24923
                     /gene="traD"
                     /locus_tag="ACHKXP_002132"
     CDS             22770..24923
                     /gene="traD"
                     /locus_tag="ACHKXP_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7647138.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            24923..30193
                     /gene="traI"
                     /locus_tag="ACHKXP_002133"
     CDS             24923..30193
                     /gene="traI"
                     /locus_tag="ACHKXP_002133"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO7647139.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMVREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAWLHTPAQQAIHLALPVVESKNLAFSMVDLLTEVKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLLQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPGSDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPITAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREITGQERDRAAITEREAALPESVLREPQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            30213..30959
                     /gene="traX"
                     /locus_tag="ACHKXP_002134"
     CDS             30213..30959
                     /gene="traX"
                     /locus_tag="ACHKXP_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO7647140.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGRNLSRHAHIRQVAINRLWGWAVIAQF
                     SYYLAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGITG
                     LLMLAVSHRLYRAEDRTERLALVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSR
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            31018..31878
                     /locus_tag="ACHKXP_002135"
     CDS             31018..31878
                     /locus_tag="ACHKXP_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7647141.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            31981..32541
                     /locus_tag="ACHKXP_002136"
     CDS             31981..32541
                     /locus_tag="ACHKXP_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO7647142.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            32674..32886
                     /locus_tag="ACHKXP_002137"
     CDS             32674..32886
                     /locus_tag="ACHKXP_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7647143.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            33131..33592
                     /locus_tag="ACHKXP_002138"
     CDS             33131..33592
                     /locus_tag="ACHKXP_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO7647144.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGWVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            33638..33847
                     /gene="hha"
                     /locus_tag="ACHKXP_002139"
     CDS             33638..33847
                     /gene="hha"
                     /locus_tag="ACHKXP_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO7647145.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDSIPKEIWKYVR"
     gene            33886..34476
                     /locus_tag="ACHKXP_002140"
     CDS             33886..34476
                     /locus_tag="ACHKXP_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO7647146.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGECWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            34716..34976
                     /locus_tag="ACHKXP_002141"
     CDS             34716..34976
                     /locus_tag="ACHKXP_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO7647147.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            35201..35275
                     /gene="tap"
                     /locus_tag="ACHKXP_002142"
     CDS             35201..35275
                     /gene="tap"
                     /locus_tag="ACHKXP_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO7647148.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            35268..36125
                     /gene="repA"
                     /locus_tag="ACHKXP_002143"
     CDS             35268..36125
                     /gene="repA"
                     /locus_tag="ACHKXP_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7647149.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVVAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(36469..36903)
                     /gene="merR"
                     /locus_tag="ACHKXP_002144"
     CDS             complement(36469..36903)
                     /gene="merR"
                     /locus_tag="ACHKXP_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFO7647150.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            36975..37325
                     /locus_tag="ACHKXP_002145"
     CDS             36975..37325
                     /locus_tag="ACHKXP_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HFO7647151.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            37339..37614
                     /gene="merP"
                     /locus_tag="ACHKXP_002146"
     CDS             37339..37614
                     /gene="merP"
                     /locus_tag="ACHKXP_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HFO7647152.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            37650..38072
                     /gene="merC"
                     /locus_tag="ACHKXP_002147"
     CDS             37650..38072
                     /gene="merC"
                     /locus_tag="ACHKXP_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HFO7647153.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            38124..39818
                     /gene="merA"
                     /locus_tag="ACHKXP_002148"
     CDS             38124..39818
                     /gene="merA"
                     /locus_tag="ACHKXP_002148"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFO7647154.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            39836..40198
                     /gene="merD"
                     /locus_tag="ACHKXP_002149"
     CDS             39836..40198
                     /gene="merD"
                     /locus_tag="ACHKXP_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HFO7647155.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            40195..40431
                     /gene="merE"
                     /locus_tag="ACHKXP_002150"
     CDS             40195..40431
                     /gene="merE"
                     /locus_tag="ACHKXP_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HFO7647156.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            40428..41075
                     /locus_tag="ACHKXP_002151"
                     /pseudo
     CDS             40428..41075
                     /locus_tag="ACHKXP_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            41210..>42460
                     /locus_tag="ACHKXP_002152"
     CDS             41210..>42460
                     /locus_tag="ACHKXP_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7647157.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYAD"
BASE COUNT        10241 a        10495 c        12223 g         9501 t
ORIGIN      
        1 ggtgtgtttt tttataggat accgctaggg gcgctgctag cggtgcgtcc ctgtttgcat
       61 tatgaatttt agtgtttcga aattaacttt attttatgtt caaaaaaggt aatctctaat
      121 ggctaaggtg aacctgtata tcagcaatga tgcctatgaa aaaataaatg cgattattga
      181 gaagcgtcga caggaagggg caagggaaaa agatgtcagt ttttcagcaa cagcttcaat
      241 gcttcttgaa ctggggcttc gtgtacatga ggctcagatg gagcgtaaag agtctgcatt
      301 taatcagact gagtttaata aattgcttct tgaatgcgtt gtaaaaacac aatcatcagt
      361 agcgaaaatt ttgggtattg agtctctcag tcctcatgtc tccggaaatc caaagtttga
      421 atatgccaat atggttgaag atatcaggga gaaggtatca tctgagatgg aacgattttt
      481 tccaaaaaat gatgatgaat aaaagaaatt tgacttcgtt caaatatcag agtttttatg
      541 atttaaaaag gtgacagtac gaaagataat tagtatatta attacgtggt taatgccacg
      601 ttaaaatttg aaattgaaaa tcgccgatgc agggagacgt gaactccctg catcgactgt
      661 ccatagaatc ctttgtgagg aggttcctat gtatccgatg gatcgtattc aacaaaaaca
      721 tgctcgtcaa atagatctgc tggaaaatct gacggcagtt attcaggatt atccaaatcc
      781 agcctgtatc agggacgaaa ctggaaaatt tattttttgc aatacgctgt ttcatgagtc
      841 atttcttaca caagatcaaa gtgctgaaaa atggcttctg tcgcagagag atttttgtga
      901 attgatctct gtcacagaga tggaagcata taggaatgag catacgcatc ttaatcttgt
      961 agaggatgtt tttattcaga acagattctg gacaatatct gtccagtcat ttcttaatgg
     1021 acacagaaat attattctgt ggcaatttta tgatgctgct catgttcgtc ataaagacag
     1081 ttataatcaa aaaacgattg tcagtgatga tatcagaaat ataatcagaa gaatgagtga
     1141 tgattcttct gtatcatcat atgtaaatga tgtcttttac ttatatagca ccggaatcag
     1201 tcataatgct atagcaagaa tattaaatat atccatctcc acatcaaaga aacacgcatc
     1261 tctgatatgc gactacttct ctgtttctaa taaagatgag ttaattatct tactctacaa
     1321 taaaaagttt atttattatt tatacgagaa agctatgtgt atcataaata cgcgttaata
     1381 aggtgttaat aaaatataga ctttccgtct atttaccttt tctgattatt ctgcaaacat
     1441 aagtggtaac cagaagataa acagcgggag gtgttattga aaagatttgg tacacgttct
     1501 gcaacaggta agatggtaaa actaaaatta cctgtagatg tggaaagtct attaattgag
     1561 gcaagtaaca gaagcggaag aagtcgatcg tttgaggcag taataagact taaagatcat
     1621 cttcaccgct atccaaagtt taacagggca gggaatatct atggtaagtc gctggttaag
     1681 tatctgacaa tgcgtctgga tgatgaaact aaccagctac ttattgcagc caaaaatcgt
     1741 agtggatggt gtaaaacaga tgaggctgca gacagagtta ttgatcattt gatcaagttt
     1801 cctgattttt ataactcgga gatattcagg gaggcagata aagaggaaga tataacattt
     1861 aatacactct agttttattc atttatccga aattgaggta acttatgaat gctgttttaa
     1921 gtgttcaggg tgcttctgcg cccgtcaaaa agaagtcgtt tttttccaaa ttcactcgtc
     1981 tgaatatgct tcgcctggct cgcgcagtga tcccggctgc tgttctgatg atgttcttcc
     2041 cgcagctggc gatggccgcc ggcagcagtg gtcaggacct gatggcaagc ggtaacacca
     2101 cggttaaggc gaccttcggt aaggactcca gtgttgttaa atgggttgtt ctggctgaag
     2161 ttctggtcgg tgctgtcatg tacatgatga ccaaaaacgt caagttcctg gccggttttg
     2221 ccatcatctc tgtatttatt gctgtgggta tggccgtcgt tggcctctga caggaaataa
     2281 aacgatgtcg ggagacgaga ataaacttaa gaaatatcgt ttcccggaaa cactgaccaa
     2341 ccagagccgc tggtttggcc tgccactgga tgaactgatc cccgcagcaa tctgtattgg
     2401 ctggggtatc acaacatcga aatatctgtt cggtattggt gcagcggttc tggtttattt
     2461 cgggattaaa aaactgaaaa aagggcgggg cagttcctgg ttacgtgacc tgatttactg
     2521 gtatatgcca acagccctgc tgcgcggtat ttttcataat gttcccgatt cgtgtttccg
     2581 gcagtggatt aaatagaact gataccagga ttgttatatg gaacacggtg cccgtttaag
     2641 taccagtcgt gtaatggcca tcgcctttat atttatgtca gtgcttattg ttctcagcct
     2701 ctctgttaac gtcattcagg ggatgaataa ctaccgtctt cagaatgagc aacgcactgc
     2761 cgtgacgcca atggcattta atgccccctt tgccgtgtca cagaacagtg ccgacgcctc
     2821 ttatttacag cagatggcgc tgtcatttat tgccctccgt ctgaatgttt catcagaaac
     2881 cgtcgatgcc tcacatcagg cgcttctgca atatatccgc ccgggcgcac agaaccagat
     2941 gaaagttatt ctggctgaag aagcgaagcg tattaaaaac gataacgtga actcagcctt
     3001 tttccagacc agtgttcgtg tctggcctca gtatggccgt gtggaaattc gaggtgtgct
     3061 taaaacctgg attggtgatt caaaaccttt cactgatatc aaacattaca tccttattct
     3121 gaagcgggaa aacggggtga cctggctgga taatttcggg gaaacagacg atgagaaaaa
     3181 ataatacggc aataatattc ggcagcctgt ttttttcctg cagcgtgatg gccgcaaacg
     3241 gtacgctggc ccccaccgtg gtgccaatgg tgaacggtgg tcaggccagt attgccatca
     3301 gcaataccag cccgaatctg tttaccgttc ccggtgatcg gattatcgcc gtgaacagtc
     3361 tggatggtgc cctgaccaat aatgagcaga ccgcctccgg cggtgtggtg gttgccaccg
     3421 tcaacaaaaa gccctttacg ttcattctgg aaacagaacg tggtctgaat ctttccattc
     3481 aggccgttcc ccgtgaaggc gcggggcgta ccattcagct ggtcagtgac ctgcgcggaa
     3541 ccggagaaga agccggtgcg tgggaaacgt ccacgcctta cgaatccctg cttgtgacca
     3601 tcagccaggc cgtccgtggc ggaaaattac ccgcaggctg gtatcaggtc ccagtgacaa
     3661 aggaaaccct gcaggccccg gcggggctgt cttcagtggc agatgccgta tggacgggga
     3721 atcacctgaa gatggtccgc tttgccgtgg aaaataaaac gctgtctgcc ctgaatatcc
     3781 gggaaagtga cttctggcag ccgggaaccc gtgccgtgat gttcagccag cctgccagcc
     3841 agttactggc aggtgcgcgc atggatgtgt atgtcatccg tgacggggag ggcaactgat
     3901 ggccagtatc aataccattg tgaaacgcaa gcagtacctg tggctgggga ttgtggttgt
     3961 cggtacagcc tccgcgattg gtggggcact gtatctgtct gatgtggaca tgtccggtaa
     4021 cggtgaaacc gtggctgaac aggagcctgt gccggatatg accggtgtgg tggatacgac
     4081 ctttgatgac aaggtgcgtc agcatgccac caccgagatg caggtgacgg cagcgcagat
     4141 gcagaagcag tatgaggaaa tccgtcgtga gctggatgtt ctgaacaaac agcgcggtga
     4201 tgaccagcgt cgtattgaaa agttgggaca ggacaatgcc gccctggcag agcaggtaaa
     4261 agccctgggt gctaatcccg tcacggcgac cggtgagcct gtaccgcaga tgcctgcctc
     4321 accgcccggc ccggaaggcg aaccacagcc aggaaacacc cccgtatcct tcccgccgca
     4381 gggcagcgtt gctgttccac cgccgacggc gttttatccc gggaatggtg tcacgccacc
     4441 accacaggtg acgtaccagt ctgtgccggt gcctaaccgg atacagcgta aggtgtttac
     4501 ccgtaatgag ggaaaacagg gaccatcact gccgtacatt ccgtcaggaa gttttgcgaa
     4561 agccatgctg attgaagggg cggatgccaa tgcctctgtc accggtaatg aatccacggt
     4621 gccgatgcag ctgcgtatca ccggcctggt ggaaatgccg aacagcaaga cgtatgacgc
     4681 aacgggatgt tttgtgggtc tggaagcctg gggggatgtg tccagtgagc gtgccattgt
     4741 acgcacccgc aatatcagtt gcctgaagga cggcaaaact attgatatgc cgattaaggg
     4801 gcatgtcagc ttccggggta aaaacggtat caagggcgaa gtggtgatgc gtaacggcaa
     4861 aatcctcggc tgggcatggg gcgcgggatt tgttgacggt attggtcagg gaatggagcg
     4921 cgcctcccag ccggctgtcg ggctgggtgc cacagccgct tacggggctg gtgatgtcct
     4981 gaaaatgggt atcggtggcg gcgcatcgaa agccgcacag acgctcagtg actactacat
     5041 caaacgtgcc gaacagtatc acccggtgat accgattggt gcgggcaacg aagtgaccgt
     5101 ggtgttccag gacggcttcc agctgaaaac cgtggaagag atggcgctgg aacgcacgca
     5161 gagcagagcg gaagaagaca atccggaaag tccggttcct gttccgccat cggctgaaag
     5221 tcatcttaac ggctttaata ctgaccagat gctgaagcag ctgggcaacc tgaatccgca
     5281 gcagtttatg tccggcagcc agggaggggg caacgatggc aaataatatg tcgtcccgtc
     5341 aggcgtgcca tgccgcccgg tatgttgtcg cccgggttct gcgaggactg ttctggtgtc
     5401 tgaagtacac cgtcattctg ccactggcca caatggccct gatgactctg tttgttttat
     5461 ggaaggacaa caccacgccg gggaaattac tggttaagga gattaacttc gtaagacaaa
     5521 cagcacctgc cggacagttc ccggtcagtg aatgctggtt ttcttcctct gattcttcag
     5581 gcagatcaga aatacagggt atctgccatt accgcgcggc tgatgctgca gattatgtca
     5641 gggagactga ccggtcactg atgcaactgg tcaccgcatt atgggcaaca ctggcactga
     5701 tgtatgtctc gctggcagcg atcaccggga aatatccggt gcgacccgga aaaatgaaat
     5761 gtatccgggt ggtaaccgca gatgaacatc tgaaggaagt ttatacggaa gacgcttccc
     5821 tgccgggaaa aatcaggaaa tgccctgttt atcttcctga tgacagaaca aacagaaaca
     5881 acggagataa aaatgaacat gcgtaatatt aatgttatta cagccctctc tgtgccgggt
     5941 aaaaccgtgt cagatgattt tatgcatgct gttctcagta actgcgccac aagaatcgta
     6001 cttcctgcac cggagaaatt cggttctgag tcattgccgg acaattttaa tatgactgct
     6061 gtcggtgtga tgaagaattc agaaatataa ggaacccttc gatgaataag ctggtttctg
     6121 atggcagcgt caaaaaaata aattatccgg tgctgtatga gtccggaata acacctccgt
     6181 tatgtgaagt cagtgcacca gaacctgatg cgggtgggaa acgcattgtg gcatatgttt
     6241 ataaatcatc acggagtacg gtttttgaaa atcctgacat tgtaaaaacc tgtacggtca
     6301 gggatttgaa gaaggatttt gtgaactgcg atgagaaagg tgagggacaa tgaaacagat
     6361 ttctttcttt attcctctgc tggggacctt attactttcc ggctgtgccg gaaccagtac
     6421 ggaatttgag tgtaacgcca ccacatccga tacctgtatg acgatggagc aggccaatga
     6481 gaaggccaaa aaactggagc ggtcctcaga agcaaagccg gttgcggcat cactgccgcg
     6541 cctggctgaa gggaacttcc ggacaatgcc ggtgcagacc gtcaccgcga ccactccgtc
     6601 cggcagcaga cctgcggtga cagcacatcc ggagcagaaa ctgctggcac ctcgcccgct
     6661 gtttaccgct gcccgggaag tgaaaacggt tgttccggtc agttcagtta cgccggtaac
     6721 acctcctcgt ccgctaagaa cgggtgagca gacggcagca ttatggatag cgccttatat
     6781 tgataaccag gatgtttatc atcagccatc cagcgtgttt tttgttatta aaccgtctgc
     6841 gtggggaaaa ccacgtatta attaactggc cctgaagttg tatatcagcc ggcaatgccg
     6901 gcgggctttc tgtattcaaa atctgcctgt aaaacgagtg ccctgtgcgt gaaaagggat
     6961 gggcggtagc gtgtcagaat atgtcgggag gaatattccg tgagtgatga agccgatgaa
     7021 gcatattcag tgacagaaca actgaccatg acaggaataa accggatacg ccagaaaata
     7081 aatgctcatg gtattcctgt ttatctctgt gaagcatgcg gaaatcctat tccggaagcc
     7141 cggcggaaaa tatttcccgg tgtgacgttg tgcgttgaat gtcaggcgta tcaggaaaga
     7201 cagagaaaac attatgcata agtcagtcgc agaacatagt gatttaattc cggatgaaca
     7261 tgagtggata ttcagaaaac agaaatcttt gtatatgcgc cgggaaatgg cgcgttaatt
     7321 acaggtattc ccttcatggc taccgcattt ctcgctttat ttttcaacta aggaattcat
     7381 gtgaataacc cacttgaggc cgtcactcag gcggttaact ccctcgtcac agcactgaaa
     7441 ctgcctgacg aatccgcaaa ggccaatgaa gttctgggcg aaatgagctt cccgcagttc
     7501 agccgtctgc tgccgtaccg tgattacaac caggaatccg gtctgttcat gaatgacacc
     7561 acgatgggct ttatgctgga agccattccc atcaatgggg cgaatgagtc cattgtggag
     7621 gccctcgatc acatgctgcg caccaaactg ccgcgcggta ttccgttgtg tatccatctg
     7681 atgtccagtc agctggttgg tgacaggatt gaatacgggc tgcgtgagtt ctcctggtca
     7741 ggtgaacagg ctgaacggtt taacgccatt acccgggcct attatatgaa agcggcagcg
     7801 acacagtttc cgctgccgga ggggatgaat ctgcccctga ccctgcgcca ttaccgggtg
     7861 tttatctcgt actgttctcc ttcgaagaaa aaaagccggg ccgacattct ggaaatggaa
     7921 aacctggtga aaatcatccg ggcgtcgtta cagggggcca gtatcaccac acagacggtg
     7981 gatgcacagg cctttatcga tattgtcggg gagatgatta accataaccc ggactccctg
     8041 tacccgaaaa gacgccagct ggacccgtat tctgatctga attatcagtg tgtggaggac
     8101 agttttgacc tgaaagtccg ggctgattac ctgacgctgg gcctgcgtga gagcggcagg
     8161 aacagtacgg cccgcatcct gaatttccat ctggcccgta acccggaaat cgcttttttg
     8221 tggaacatgg ccgacaacta cagcaacctg ctgaacccgg agttgtccat ctcctgcccg
     8281 ttcatcctga cgctgactct ggtggtggaa gaccaggtga aaacccacag cgaagccaac
     8341 ctgaagtaca tggacctgga gaaaaagtcg aagacctcct atgccaaatg gtttccgtcc
     8401 gtggagaaag aagcgaagga gtggggggaa ctgcgtcagc ggctgggctc cggtcagtcc
     8461 tctgtcgtgt cctacttcct caacatcaca gccttctgca aggacaataa tgaaacggca
     8521 ctggaagtgg agcaggacat cctgaacagc ttccgtaaaa acggttttga gctgatttca
     8581 ccgcgcttta accacatgcg caatttcctg acctgtctgc cctttatggc cgggaaaggg
     8641 ttgtttaaac agctgaaaga ggccggagtg gtacagcgcg cagagagctt taatgtggcc
     8701 aacctgatgc cgttagtggc ggataacccc ctgacaccgg caggtctgct ggcacccacc
     8761 taccgtaacc agctggcgtt tatcgatatt ttcttccggg ggatgaataa caccaactat
     8821 aacatggcgg tctgtggcac ctccggggcc ggtaaaaccg ggctgataca gccacttatc
     8881 cgcagcgtgc tggactccgg aggctttgcc gtggtgttcg acatggggga tggatacaag
     8941 tccctgtgtg agaacatggg cggggtgtat ctggacggtg aaaccctgcg ctttaacccg
     9001 tttgcgaaca tcaccgatat tgaccagtca gcggagcgtg tccgtgacca gctgtcggtg
     9061 atggccagcc ccaacggtaa cctggatgaa gtgcatgaag gtctgctgct gcaggcagtc
     9121 agggcatcat ggctggccaa agagaacagg gcacgtattg atgacgtggt ggatttcctg
     9181 aaaaacgcca gtgacagcga gcagtatgcc gagtcaccga ccatccgcag ccgtctggac
     9241 gaaatgattg tgctgcttga ccagtacact gctaacggca cttatggcca gtatttcaac
     9301 tccgatgagc cgtccctgcg ggatgatgcg aaaatggtgg tgctggagct gggcggactg
     9361 gaagaccgtc cgtccctgct ggttgcggtg atgttcagcc ttatcattta cattgagaac
     9421 cggatgtacc gcacgccgcg taacctcaag aaactgaacg tcattgatga aggctggcgt
     9481 ctgctggact tcaaaaatca caaggtcggt gaatttattg agaaaggcta ccgtaccgcc
     9541 cgccgtcata ccggtgccta tatcaccatc acccagaaca tcgtcgactt tgactctgac
     9601 aaggcctcca gtgcggcccg tgcagcatgg ggtaactcct cctacaaaat tatcctcaaa
     9661 cagagtgcga aggagttcgc gaaatacaac cagctgtatc cggaccagtt cctgccactg
     9721 cagcgtgaca tgattggtaa gttcggtgcg gccaaagatc agtggttcag ttccttcctg
     9781 ctgcaggtgg aaaaccattc ctcctggcac cgcctgtttg tggacccgtt aagccgcgcc
     9841 atgtacagct ctgacggccc ggattttgag tttgtgcagc agaaacgtaa agaggggctg
     9901 agtattcatg aggcagtgtg gcagctggcg tggaagaagt cagggccgga gatggcttcg
     9961 ctggaagcct ggctggaaga acatgagaaa tacaggagtg ttgcatgacc acaacgcaaa
    10021 aaacgactga cgttacagcc ccccgacgca gccactggtg gtggacggtg ccgggatgcc
    10081 tggcaatggt gttactgaac gcagccatca gttacggcat tgtcagactg aacgcaccgg
    10141 tgactgccgc cttcaacatg aaacagaccg tggatgcgtt ttttgacagc gccagtcaga
    10201 aacaactgtc agaagcacag tcaaaagccc tttcagcgcg ttttaacacg gcactggaag
    10261 ccagcctgca ggcatggcag cagaaacacc acgcggtcat tctggtgtcg cctgccgtgg
    10321 tacagggggc tccggatatc acccgggaaa tccagcagga tatcgcccgg cgaatgaggg
    10381 cggagccatg agatgccggg ggctgattgc cctgctgata tgggggcaga gtgtggccgc
    10441 cgccgatctt ggtacctggg gcgatctgtg gccggtaaag gagccggaca tgctgacggt
    10501 gatcatgcag cgcctgacgg cactggagca gtccggtgag atgggccgga aaatggatgc
    10561 ctttaaggag cgggtgatcc gcaacagtct gcggccccct gccgtgcccg gtatcggacg
    10621 cacggagaaa tacggcagcc ggttgtttga cccgtccgtc agactggctg cggatatccg
    10681 ggataacgaa gggcgggtgt ttgcccgtca gggtgaagtg atgaatccgc tgcagtatgt
    10741 tccttttaac cagacgctgt atttcatcaa tggcgatgat ccggcgcagg tggcctggat
    10801 gaaacgccag acgccgccca cactggagag caaaattatc ctcgtgcagg gcagtatccc
    10861 ggagatgcag aagtctctgg acagccgtgt ttactttgac cagaacggtg tgctctgcca
    10921 gcgcctcggc attgatcagg tcccggcacg ggtgagcgcc gttcccggcg atcgcttcct
    10981 gaaggtggaa tttattccgg cagaggaggg cagaaaatga agcgaagcct gtggctgctg
    11041 atgttattcc ttctcgccgg tcatgtccct gcagcgtctg cggattctgc ctgtgagggg
    11101 cgttttgtaa acccgatcac ggatatctgc tggagctgta ttttcccgct ctcgctgggc
    11161 agtatcaaag tcagtcaggg taaggtcccc gacacggcga acccgtcgat gcccattcag
    11221 atttgtccgg caccgccgcc gctgttcagg cgtatcgggc tggccattgg ttactgggag
    11281 ccgatggcgc tgacggacgt cacccgatca ccgggatgca tggttaacct gggcttcagc
    11341 ctgccggctt ttggtaaaac ggcacaggga acggcgaaaa aggatgagaa gcaggtgaac
    11401 ggggcgttct atcacgttca ctggtacaaa tacccgctga cgtactggct taacatcatc
    11461 acatcgctgg gctgtctgga aggtggtgac ctggatatcg cttatctttc tgaaatcgac
    11521 cccacctgga cggacagcag cctgaccacc attctcaatc cggaagctgt catctttgcc
    11581 aatccgatag cacagggggc ctgtgcagca gatgccattg ccagcgcctt taatatgcct
    11641 ctcgatgttc tgttctggtg tgccggttcg cagggcagta tgtacccgtt caatggctgg
    11701 gtgagtaatg agtccagtcc gttgcagtcc tccctgctgg tcagtgaacg catggcgttc
    11761 aagctgcacc gtcagggcat gattatggaa accatcggga aaaataacgc cgtctgtaat
    11821 gaatatccgt ccccaatcct gcccaaagaa cgctggcgtt accagatggt gaatatgtac
    11881 ccggacagcg gacagtgcca cccgttcggg cgcagtgtga cccgctggga aaccgggaaa
    11941 aatccgccca atacaaagaa aaacttcggc tacctgatgt ggcgtaaacg taactgtgtc
    12001 ttcctgtgag gtgaatgatg aagctgagta tgaaatctct ggcagcactg ctgatgatgc
    12061 tgaacggggc ggttatggcg tcagaaaacg tgaacactcc tgaaaaccgc cagttcctga
    12121 agcagcagga aaatttaagc agacaactgc gtgaaaaacc tgaccatcag ctgaaagcct
    12181 gggcggagaa gcaggtgctg gaaaaccccc ttcagcgttc agataaccat ttcctggatg
    12241 agctggttcg taaacagcag gcttcgcagg acgggaaacc ccggcagggt gctctgtatt
    12301 ttgtgtcgtt ttccattccc gaagaggggc tgaaacgaat gctgggcgaa acccggcact
    12361 acggtatacc cgccacactg cggggcatgg tgaacaatga cctgaagacc accgctgaag
    12421 ccgtgctgtc cctggtgaaa gacggcgcga ctgatggtgt tcagatcgac ccgacgctgt
    12481 tttcacagta cggcattcgc agtgtaccgg cactggtggt gttctgcagt cagggatacg
    12541 acatcatccg gggaaacctg cgggtcggcc aggcgctgga gaaagtggcc gccacgggtg
    12601 actgccggca ggtggctcat gatttactgg cagggaaagg agtttccggg aaatgaaacg
    12661 tattttacct ctgatactgg ctctggttgc cggcatggca caggctgaca gcaacagtga
    12721 ttaccgggcc ggttctgatt ttgctcacca gatcaaaggg cagggaagca gcagtattca
    12781 gggctttaag ccacaggaga gtatccccgg ctataacgcg aatccggacg agacaaaata
    12841 ctacggtggc gtgactgccg gcggggatgg cggcctgaaa aatgacggca ccacggaatg
    12901 ggcgaccggt gaaaccggaa aaaccatcac agagtcattc atgaacaagc cgaaagacat
    12961 tctttcaccg gatgcaccgt ttatccagac cggcagggat gtggtgaacc gggctgacag
    13021 cattgtggga aacaccgggc agcagtgcag tgcgcaggag attagccgga gtgaatacac
    13081 gaattacacc tgtgagcggg atttacaggt ggagcagtac tgcacacgaa ctgcccgtat
    13141 ggagctgcag gggagtacca catgggaaac ccggacgctg gagtatgaaa tgagtcagct
    13201 acctgcacgt gaagtgaatg gtcagtatgt tgtctccatt acctctcccg ttaccggtga
    13261 aattgtcaat gcgcattaca gctggagtcg tacttacctg cagaagagtg tacctatgac
    13321 aattacagta ctgggaaccc cactgagctg gaatgccaaa tactcagcag atgcctcatt
    13381 tacaccagta cagaaaacac tgactgccgg tgttgctttt acgtcatctc accccgtccg
    13441 ggtcgggaat acaaaattca aacgtcatac ggcaatgaag ctgcgtctgg ttgtcagggt
    13501 aaaaaaagcc tcgtacaccc cgtatgttgt ctggtctgaa agctgcccgt tcagcaagga
    13561 gctggggaaa ctgacaaaaa cagaatgtac ggaggctggc gggaacagga cgctggtgaa
    13621 agacggtcag tcatacagca tgtaccagag ctgctgggca taccgggaca cgtatgtcac
    13681 acagtcagca gacaagggaa cctgtcagac ctacgccgac aatcctgcct gtaccctggt
    13741 gtcacaccag tgcacctttt actccgaaga aggtgcctgt ctgcatgaat atgccacgta
    13801 ctcctgtgag tcaaaaacat ccgggaaagt gatggtctgt gggggagacg tcttctgtct
    13861 cgatggcgaa tgcgataagg ctcagagcgg gaagagtaat gattttgccg aagccgtttc
    13921 tcagctggcg gcactggccg cagcaggtaa ggatgttgcg gcgctgaacg gggtggatgt
    13981 ccgtgctttc accggtcagg cgaaattttg taagaaggct gcagccggat acagcaactg
    14041 ctgtaaggac agcggctggg gacaggatat cgggctggcc aaatgcagca gtgatgaaaa
    14101 agccctggct aaagcaaaat caaacaaact gaccgtcagt gtcggagagt tctgttcgaa
    14161 gaaagtgctg ggtgtctgcc tggagaaaaa acgcagttac tgtcagtttg attcgaagct
    14221 ggcgcagata gtccagcagc aggggcgcaa cggacagctg cgtatcagtt ttggcagtgc
    14281 gaagcatcct gactgtcggg ggattacggt tgatgagctg cagaaaattc agttcgacag
    14341 actggatttc actaacttct acgaagacct gatgaataac cagaaaatcc cggacagtgg
    14401 tgttctgacg cagaaagtga aagagcagat tgctgaccag ctgaaacagg caggacagta
    14461 aggtagggaa aataaccggg gaaaatgatg aaagtcatat tcacgtcgaa ccgttttatt
    14521 gattttctta tccggctttt gattacagcg attgtcattt ccccggtaat tatctggtcg
    14581 tgggatacgg taaaagaaac cactgcaggt gagatacttg ctgctgcttt cgttattttt
    14641 tactccggtg tgctcctgtt tattctgtat ttttgttttt ctgccctgac tgacctgcag
    14701 aaacctgatg aaagaaaaag tgatgaaagg aatgaagatg aataaagcat tactgccact
    14761 gttactctgc tgctttattt ttccggcgtc aggaaaagat gcaggctggc agtggtataa
    14821 cgagaaaata aatccgaagg aaaaagaaaa taaaccagta cctgcagccc cccgtcagga
    14881 gccggacatt atgcagaaac tggccgcact gcagacggca acaaagcggg cgctgtacga
    14941 agctattctg tatcccggcg tggataattt tgtgaaatat ttccggctgc agaattactg
    15001 gactcagcag gccgggcttt tcaccatgag cgccaaaaaa gccatgctgg cgcatcccga
    15061 actggactat aacctgcagt acagccatta caacggcacg gtccggaacc agctggcggc
    15121 agaccaggtg cagcagcgac aggccattgc gaaactggct gaacactacg gcatcatgtt
    15181 cttttaccgg gggcagggcc ccatcgacgg gcagctggcg caggtcatta atggcttcag
    15241 ggatacctat ggtctgagtg ttattcccgt ttccgtggat ggcgtgatta atccgctgtt
    15301 gccggattcc cggactgacc aggggcaggc gcagcgcctc ggcgtgaaat atttcccggc
    15361 catgatgctg gttgacccga aacagggcag tgttcgcccg ttatcatacg gttttatttc
    15421 gcaggacgac ctggcaaaac aattcctgaa cgtttctgaa gattttaaac ccaattttta
    15481 atcgcggatt tgatttatga gtgaagattg tttgaaaatg tttacaggtg ttgttctgtt
    15541 aatatttgtc attattgccg gttatttctt ttctgagcgt aatgacagga aaatgtttct
    15601 cctgagctca ctggttttcc ttgttattaa tatcgcgtgt ttatatgtgc tgactgccag
    15661 tctctggttt ctgtgtggtg caattatgaa tcagggcgca gcaatggttg ttccaatggt
    15721 tattggctca ttaccgaacg ttacgagctt cgacgggttc agaagaatat ttatctgtat
    15781 tatgttgtca tcagtatggt ccggagtgat gtggtttttt ataagggggc ttatgacagg
    15841 ctaagtcata taaaacggta ataaaaacca tacaaaataa tgaggttgat ctgattaata
    15901 tcacacaaac gcgaatatta cagacataaa tactctcatg taaataataa ccggagttta
    15961 acatgataag taaacgcaga ttctctttac cccggcttga tattacggga atgtgggtat
    16021 tttccctggg tgtctggttt catatcgtcg cccgtcttgt ttacagcaaa ccctggatgg
    16081 ctttttttct ggcggagttg attgcggcca tccttgtgct gttcggcgca taccaggtgc
    16141 tggatgcatg gattgcccgt gtcagccggg aagagcgtga ggcactggaa gccaggcaac
    16201 aggccatgat ggaagggcag caggagggca gacatgtctc tcattaaatc actgttgttc
    16261 accctgttgc tggcagccgc tgccgtgcag gcctccaccc gggatgaaat tgaacgactc
    16321 tggaatccgc agggcatggc cgcgcagcct gcacaaccgg cagcaggcac gtcagccagg
    16381 acagaaaagc cggctccccg ctggttccgc ctcagcaacg gcaggcaggt taacctggcc
    16441 gactggaagg tggtgctgtt tatgcagggg cattgccctt actgtcacca gtttgacccg
    16501 gtactgaaac agctggcaca gcagtacggc ttttccgttt ttccctacac cctggatggt
    16561 cagggggata ccgcctttcc ggaagcatta ccggtgccac cggacgtgat gcagaccttc
    16621 ttcccgaata tcccggtggc cacaccgacc accttcctgg tcaacgtcaa cacgcttgag
    16681 gcattaccgc ttttacaggg ggcaacggat gccgccggtt ttatggcgcg gatggatacc
    16741 gttctgcaga tgtacggagg aaaaaaaggt gcgaaataag caggtggtgt tactcattgc
    16801 cggaatatcc ggcatcgcga cgggaataat cgtcagcctg aatatcccct ttatccatca
    16861 ggggcttttt tatcccgcca gccctgtaga aattgtcgtt tcgctgtgcc ttaccttttc
    16921 tgtttctgtt gtgttttttg tgggggcaat tgtgggatgg atttcggtgt ctgaaatata
    16981 ttacagccgc aggactggac ggaaatatcg ggatattcct gctgaaatgt cgtatgcgcc
    17041 gggagaacaa attaagggag ggcaagaatg atgccacgca ttaaacctct tctggttctt
    17101 tgtgctgctt tactgacggt cacacccgca gcgtcagcgg atgtgaacag cgacatgaat
    17161 cagttcttta acaagctggg cttcgcctcc aataccacac agccgggcgt ctggcagggg
    17221 caggccgccg gttatgctta cggtggctcc ctgtatgccc gtactcaggt caaaaatgtt
    17281 cagctgattt ccatgacgct gccggatatc aatgccggat gtggcggcat cgacgcctat
    17341 ctcggctcgt tcagttttat taatggcgag cagctgcagc gttttgttaa gcagattatg
    17401 agcaatgctg ccggttactt ttttgacctt gccctgcaga caacggtgcc ggaaatcaaa
    17461 accgcaaaag acttcctgca gaaaatggca agcgacatta acagtatgaa cctcagttcc
    17521 tgccaggctg cacaggggat tatcggcggg ctcttccccc ggacgcaggt gtcccagcag
    17581 aaagtctgtc aggacattgc cggtgagagc aatatttttg ctgactgggc ggcttcccgg
    17641 cagggatgta ccgttggcgg gaaatctgac agtgtcaggg ataaagccag cgacaaggat
    17701 aaggagcggg tgaccaaaaa catcaacatc atgtggaatg cgctttccaa aaacagaatg
    17761 tttgacggca acaaagagct gaaagagttt gtgatgacgc tgaccggctc actggtgttt
    17821 gggcctaacg gcgaaatcac acccttgtca gccagaacca ctgaccgctc aattatccgg
    17881 gccatgatgg aaggcggcac agcaaaaatt tatcactgca acgattctga taaatgcctg
    17941 aaagtggtgg cagacacacc ggtgaccatc agccgggata atgcactgaa gtctcagatt
    18001 actaaacttc tggccagcat tcagaacaag gctgtcagtg acacgcctct ggatgacaag
    18061 gaaaaaggct ttatttccag taccaccatc cccgtcttca aatacctggt tgacccgcag
    18121 atgctcggtg tttccaacag tatgatttac cagctgacgg actatatcgg ttacgacatc
    18181 ctgctgcagt acattcagga gctgatacag caggccaggg cgatggtggc cacaggaaat
    18241 tatgacgaag cagttatcga acatattaac gacaacatga atgatgccac ccggcagatt
    18301 gcggcgtttc agtcacaggt gcaggtacag caggatgcgc tgctggttgt cgatcgtcag
    18361 atgagctaca tgcgtcagca gctttccgcc cgcatgctca gtcgttacca gaacaactat
    18421 cacttcggag ggagcacgct gtgaatgaag tttatgtgat tgccggtggt gagtggttgc
    18481 ggaataacct gaacgccatt gccgccttta tgggaaccag gacgtgggat tccattgaaa
    18541 aaattgcgct cacattgtct gttctcgcgg tggccgtgat gtgggtacag cggcacaacg
    18601 tgatggattt gctgggctgg gtggccgtgt ttgtgcttat cagcctgctg gttaatgtcc
    18661 gcacatcggt gcaggttatt gataacagtg acctggtcaa agttcaccgg gtggataatg
    18721 tgccggtcgg actggcgatg ccactttcac tgacgacccg tatcgggcat gcaatggtgg
    18781 ccagttacga gatgatcttc acgcaaccgg acagcgtcac ctacagcaaa acggggatgc
    18841 tgtttggcgc agaactggta tcaaaaagca ccgacttttt gtcccggaat ccggaaatca
    18901 ccaatctttt ccaggattat gtgcagaact gcgtgatggg ggatatttac ctgaaccaca
    18961 aatatacgct ggaggagctg atggcctccg ctgatcccta cacgctgatt ttttcccgcc
    19021 ccagcccgct gcggggcgtt tatgacagca ataataattt cgtaacctgt aaggacgctt
    19081 cggtttcgct gaaagacaag ctgaatctgg acacacagag tggcggaaaa acctggcatt
    19141 attatgccca gcagctattt ggcggacgcc ctgatcctaa cctgctgttc agtacgctga
    19201 ttggcgacag ttacagttac ttctatggct caagtaaatc agccagccag atcatccgcc
    19261 agaatgtcac catcaatgcg ctgaaggagg ggatcaccag ttatgccgca cgtaatggtg
    19321 attcggccag cctggtgaat ctggccacca cgtcatcgat ggagaaacaa cgtctggcac
    19381 atgtctccat cggtcatgtg gcgatgcgga cactgcccat gacgcagacc atcctgacgg
    19441 ggatcgccat cggtattttc ccgctcctgg tactggcggc tgtcttcaac aaactgacgc
    19501 tgtccgtcct gaaaggctat gtgtttgccc tgatgtggtt gcagtcatgg ccaatgttgt
    19561 acgccattct gaacagcgcc atgacatttt atgcaaagca gaatggtgca ccggtggtgc
    19621 tctcggaaat ttcacagata cagctgaaat actccgatct ggcttccact gccgggtatc
    19681 tttccatgat gatcccgcca ttatcctgga tgatggtcag ggggctcggg gcaggatttt
    19741 ccagtgtgta cagccatttc gcttcctctg ctatcagccc gaccgccagt gcagcaggca
    19801 gtgtggttga cggtaattac tcctacggca acatgcagac ggaaaacgtg aacggcttca
    19861 gctggagcac caacagcacc acgtcgtttg gtcagatgat gtaccagacc ggcagtggcg
    19921 caaccgccac acagacccgt gacggtaata tggtgatgga tgcaagcgga gcgatgtccc
    19981 ggttaccggt cggcatcaat gcaacgcgtc agattgcggc ggcacaacag gaaatggccc
    20041 gggaagcatc gaacagagca gaaagtgccc tgcatgggtt cagcagcagt attgccagtg
    20101 cctggaacac gctcagtcag tttggttcta accgggggag cagtgattct gtcaccggtg
    20161 gtgctgacag cacgatgagt gcacaggact ccatgatggc cagccgtatg cgcagtgcag
    20221 tggaaagcta tgcgaaggcg cataatatca gtaatgagca ggcgactcag gaactggcat
    20281 caacaagtac caggctatct ggtggaatgt atgctgatgc tcatgctgaa tggggggtta
    20341 aacctaaaat tcttggtgtt ggtggtgggg taggcatcag aggtggattt aaggggagcc
    20401 tggatgcaac ggatgatgac tcacattcag caagtagtgg cagcagggct acccatgatg
    20461 ctcgccatga tatcgatgct aaagccagta aggactttaa agaggccagc gattacttta
    20521 ccagtcgcaa agtcagtgaa tccggcagcc atactgacaa taatgccgat tcccgcgtgg
    20581 accagttgtc tgcagccctg aactcagcga aacagagtta cgaccagtac acgacgaata
    20641 tgacccgcag ccatgaatat gctgaaatgg catcccgtac tgaaagcatg agtgggcaga
    20701 tgagcgaaga tttgtcgcaa cagtttgcac tgtatgtgat gaaacacgca ccgcaggatg
    20761 ccgaagctat tctgacaaac accagttcac cggagattgc agaacgccgt cgggcgatgg
    20821 catggtcttt tgttcaggaa caggtgcagc ctggtgttga taatgcctgg cgtgaatccc
    20881 gtggggatat tggtaaagga atggagagcg taccttcggg tggtggcagc caggatatta
    20941 ttgctgacca tcaggggcat caggccatta ttgatcaaag aacgcaggac agtaatatcc
    21001 gtaatgatgt aaaacatcag gttgataata tggtcacaga atataaaggg aatatcggag
    21061 atactcagaa tagtattcat ggcgaagaaa atattgtaga cagacaatat tcagagttaa
    21121 aaaataatca caaacaggaa gaaattcaac aaaacaacag atataatgaa gaaaataaac
    21181 gacagaaatt aatgccaaca ccgtctgaag atgcattaaa acagatggtg gatgataaga
    21241 aagaacgatt gaaggggcca ttatgatcac tcactatgat ataaaaatgg agatgcaaaa
    21301 actaaaggag gtactctctg tagagggtgt taatatccca tcattattac aagtcataaa
    21361 gccgggaact tatgtgtttt tatgggtttt attatggcca acttttctgc gtttagtatc
    21421 ggttaaaagt gatgtcaggg atgttggctt tgatatatgt gctagtggaa tgatggggtt
    21481 cctgcttttt gtggctatta caaatggaat gatgttatac cttgcgattc ctgatagttt
    21541 cagaaaggat tcaaaaataa taaattttat gtattctaag agtaagacat atattttgtt
    21601 gtttttgatt gttttttcta tggtttcgtt tatgcattct atcttatatg tttttgcact
    21661 aatgattaca ttcatattat ttttcttggt gtataccata gatataaatc gttataatct
    21721 ctcagctata gcctcagtaa taggactgtt caaaaaagag tctgttagtt aaaaggatat
    21781 tttggatgaa aacaaaaaaa ttgatgatgg ttgcactggt cagttccact ctggcccttt
    21841 cagggtgtgg tgcgatgagc acagcaatca agaagcgtaa ccttgaggtg aagactcaga
    21901 tgagtgagac catctggctt gaacccgcca gcgaacgcac ggtatttctg cagatcaaaa
    21961 acacgtctga taaagacatg agtgggctgc agggcaaaat tgctgatgct gtgaaagcaa
    22021 aaggatatca ggtggtgact tctccggata aagcctacta ctggattcag gcgaatgtgc
    22081 tgaaggccga taagatggat ctgcgggagt ctcagggatg gctgaaccgt ggttatgagg
    22141 gcgcagcagt tggtgcagcg ttaggtgccg gtatcaccgg atataattcc agttctgccg
    22201 gtgccacact cggtgtgggc cttgctgccg gtctggtggg tatggctgca gatgcgatgg
    22261 tggaagatgt gaactatacc atgatcacgg atgtgcagat tgcagagcgt actaaggcaa
    22321 cggtgacaac ggataatgtt gccgccctgc gtcagggcac atcaggtgcg aaaattcaga
    22381 ccagtactga aacaggtaac cagcataaat accagacccg tgtggtctca aatgcgaaca
    22441 aggttaacct gaaatttgaa gaggcgaagc ctgttctcga agaccagctg gccaaatcaa
    22501 tcgcaaatat tctctgatta tcttccggag gctggtctga ccggcctcct gactgacgcc
    22561 tcgctttgct cgttgtccaa ccccggaaag aaataaaata aattttattt ctccccggcg
    22621 tttccgaaat aaatatgaaa acactgagcg gttgtgcaaa ttctttgtgc gaccgtgaag
    22681 tgttgccggc gaagccggaa tttaacggtt attaatcttt tcatcattaa taatcagtcc
    22741 gactgacttt ttttcttcgg aatatcatca tgagttttaa cgcaaaggat atgacccagg
    22801 gcggtcagat tgcgtccatg cgtatccgca tgttcagcca gatcgccaat atcatgcttt
    22861 actgcctgtt tatttttttc tggatactcg ttggtctggt tttatgggta aaaataagct
    22921 ggcagacgtt tgtgaacggc tgtatttact ggtggtgtac cacgctggaa ggcatgcggg
    22981 atttaatcaa gtcccagccg gtatatgaga tccagtatta cggcaaaacc ttccggatga
    23041 acgctgctca ggtactgcat gataaatata tgatctggtg cggagagcag ctatggtccg
    23101 cattcgttct ggctacagtt gtggcactgg ttatttgcct gattaccttc tttgttgtct
    23161 cctggattct ggggcgtcag ggtaaacaac agagcgaaaa tgaagtcaca ggtggtcgtc
    23221 agctgacgga gaacccgaaa gacgttgccc ggatgctgaa aaaagacggc aaggactccg
    23281 atatccggat tggcgacctg ccgattatcc gggattccga aatccagaac ttctgtctgc
    23341 acggcacggt cggggccggt aagtcggaag ttatccgtcg tctggccaac tacgcccgtc
    23401 agcgtggaga tatggtggtg atttatgacc gttcagggga atttgttaaa agttactatg
    23461 atccctccat cgataaaatc ctgaatccgc tggatgcccg ctgtgccgcc tgggatttgt
    23521 ggaaggagtg cctgacacag ccggattttg ataataccgc gaacacgctg atcccgatgg
    23581 gcacaaaaga ggacccgttc tggcagggtt caggacgtac catttttgcg gaagcggcgt
    23641 acctgatgcg taatgacccc aaccgcagct acagcaaact ggtggacaca ctgctttcca
    23701 tcaaaatcga aaaactgcgt accttcctgc gtaattcacc ggcggccaac ctggtggaag
    23761 agaaaattga gaaaacggcg atttccatcc gtgctgtgct gaccaactac gtgaaagcca
    23821 tccgttacct gcaggggatt gagcataacg gtgagtcttt taccatccgt gactggatgc
    23881 ggggtgtccg ggaagatcag aaaaacggct ggctgtttat ctcgtcgaat gccgacaccc
    23941 atgcctccct gaagccggtg atctccatgt ggctgtccat tgccattcgt ggtctgctgg
    24001 caatgggaga aaaccgtaac cgtcgtgtgt ggtttttctg tgatgagcta cccacgttac
    24061 acaaactgcc ggacctggtg gagatcctgc cggaagcccg taagttcggt ggctgttatg
    24121 tgtttggtat ccagtcctat gcccagctgg aagatatcta cggtgagaaa gcggctgcca
    24181 cgctgtttga cgtcatgaac acccgtgcct ttttccgttc tcccagccat aagattgcag
    24241 agttcgctgc cggtgagatt ggtgagaaag agcacctgaa agccagcgag cagtactcct
    24301 acggtgctga tccggtacgt gacggggtat cgaccggtaa ggatatggag cgccagacgc
    24361 tggtcagtta ttccgacatt cagtctctgc cggatttgac ctgttatgtc accctgcccg
    24421 gaccgtatcc ggcagtaaaa ctctcactga aatatcaggc acgtccgaag gttgcccctg
    24481 agtttattcc gcgtgacatc aacccggaaa tggagaaccg cctgagcgcc gtacttgccg
    24541 caagggaagc agaaggtcgt cagatggcca gcctcttcga accggatgtg ccggaggttg
    24601 tttccggaga agacgtgact caggctgaac agccgcaaca gccggtgtct cctgccatca
    24661 acgataagaa gtcagattca ggtgtgaatg ttccggcagg ggggatcgag caggagctga
    24721 aaatgaaacc ggaagaagag atggaacagc aactgccacc cgggatcagt gaatccggtg
    24781 aagtggtgga tatggccgct tatgaggcat ggcaacagga aaatcatccg gacatccagc
    24841 agcagatgca gcgtcgtgaa gaggtgaaca ttaatgtgca ccgggagcgc ggggaggatg
    24901 ttgagccggg agatgatttc tgatgatgag tatcgcgcag gtcagatcgg ccggaagtgc
    24961 cggtaactat tataccgaca aggataatta ctatgtgctg ggcagcatgg gagaacgctg
    25021 ggccggcagg ggggctgaac agctgggact gcagggcagt gtcgataagg atgtttttac
    25081 ccgtcttctg gagggcaggc tgccggacgg agcggatcta agccgcatgc aggatggcag
    25141 taacaggcat cgtcccggct acgatctgac cttctccgcc cccaaaagtg tctccatgat
    25201 ggccatgtta ggtggcgata agcgcctgat tgatgcacat aaccaggccg tggactttgc
    25261 tgttcgtcag gtggaggcgc tggcctccac acgggtgatg acggacggac agtcagaaac
    25321 ggtgctgacc ggtaatctgg tgatggcact gtttaaccac gacaccagtc gcgatcagga
    25381 accacagtta cacacgcatg cggtggtggc taatgtcacg cagcataacg gcgaatggaa
    25441 gacacttagc agtgacaaag tggggaaaac ggggttcatt gagaatgtgt acgctaatca
    25501 gattgccttt ggcaggctct accgggaaaa actgaaagag caggttgagg cgctgggcta
    25561 tgaaaccgaa gtggtgggta agcacggtat gtgggagatg ccgggcgtac cggtggaggc
    25621 cttttccgga cgctcacagg caatccggga ggccgtcggg gaagatgcct cgctgaaatc
    25681 ccgggatgtg gcggcactgg atacgcgaaa atccaaacag cacgtcgatc ctgaagtcag
    25741 aatggctgaa tggatgcaga cgctgaagga aaccgggttc gacatccggg catatcgtga
    25801 cgcggcggat cagcgtgcgg agacccgcac gcaggcgccc ggggctgttt cacaggaggg
    25861 gccggatgtg cagcaggcgg tgacacaggc cattgccgga ttaagtgaac gaaaagtgca
    25921 gtttacgtac acggacgtgc tggccagaac ggtcggcata ctgccgccgg aaaatggtgt
    25981 gattgagcgg gcccgcgccg gtatcgatga ggccatcagc cgtgagcagc tcattcccct
    26041 cgatcgtgag aaggggctgt tcacgtccgg gattcatgtg ctcgatgagc tgtcagtccg
    26101 ggcactcagt cgtgacatca tgaaacagaa ccgggtgacc gtacatccgg agaaaagtgt
    26161 cccccggacg gctggttaca gcgatgcggt cagtgttctg gcacaggacc gtccgtcgct
    26221 ggccattgtg tccgggcagg gcggtgcagc cgggcagcgt gagcgggtgg ctgaactggt
    26281 catgatggtc cgggagcagg ggcgggaggt gcagattatc gctgctgacc gtcgctcgca
    26341 gatgaacctg aagcaggatg aacggctgtc cggtgagctg ataaccggac gtcgtcagtt
    26401 gctggaaggc atggccttca cgccgggcag tactgttatc gttgaccagg gcgaaaaact
    26461 ctccctgaaa gagacgttaa ccctgctgga cggtgccgca cgtcataacg tacaggtcct
    26521 gataaccgac agcgggcagc gaaccggtac aggcagtgcg ctgatggcca tgaaggatgc
    26581 cggggtgaac acatatcgct ggcagggggg agaacagcga ccggccacca tcatcagtga
    26641 accggaccgt aatgtccgct atgcccggct ggcaggagat tttgcggcca gcgtgaaagc
    26701 cggagaagag agcgtggcac aggtcagcgg ggtacgggaa caggccatac tgacacaggc
    26761 cattcgcagt gagctgaaaa cacagggcgt gctcggacat caggaggtga ccatgacggc
    26821 actgtcaccg gtctggctgg acagccggag ccgttatctg cgggatatgt accgcccggg
    26881 aatggtgatg gagcagtgga acccggagac acgcagtcat gaccgctatg tgatagaccg
    26941 ggtgacggcg cagagtcaca gcctgaccct gcgggatgcg cagggtgaaa cgcaggtggt
    27001 gcgtatttct tctctggaca gcagctggtc gctgttccgg ccggaaaaaa tgccggtggc
    27061 agacggcgag cgtctgaggg tgacagggaa aattcccgga ctccgcgtct ccggcggtga
    27121 ccgcctgcag gtggcatccg tcagtgaaga tgcgatgacg gttgttgtgc cggggcgggc
    27181 tgaaccggcc accctgcctg tggctgattc accgttcacg gcactgaagc tggagaacgg
    27241 ctgggtggaa acgcccgggc attccgtcag cgacagtgcg aaggtttttg cctccgtcac
    27301 acagatggca atggacaacg ccaccctgaa cggtctggcc cgcagcggtc gtgatgtccg
    27361 gctgtattcc tcactggatg aaacccgtac tgcggaaaaa cttgcccgcc atccctcctt
    27421 tacggtggtt tctgagcaga taaaggcgcg tgccggtgag acattgctgg aaaccgctat
    27481 cagtctgcag aaagcctggc ttcacacgcc ggcacagcag gctattcatc tggcgcttcc
    27541 tgtggtggaa agtaaaaacc tggccttcag catggtggac ctgctgacag aggtgaagtc
    27601 gtttgctgca gaaggaacca gttttactga actgggaggg gaaatcaatg cgcagataaa
    27661 acgcggtgat ttactgtatg tggatgtggc aaaaggctat ggcacaggcc tgctggtttc
    27721 ccgtgcgtcg tatgaggcag aaaagagcat tcttcgccat attctcgaag gtaaggaggc
    27781 ggtcacgccg ctgatggaga gagtacctgg cgaactcatg gagaaactga catcaggaca
    27841 gcgtgccgct acccgcatga tactggaaac gtccgaccgt ttcacggtgg tgcagggcta
    27901 tgccggtgtg ggtaagacca cacagttccg ggcggtgatg tcagccgtga acatgctgcc
    27961 ggagagtgaa cgtccccgtg tcgtggggct ggggcccacg caccgtgcgg tcggtgagat
    28021 gcgcagcgcc ggcgtggatg cacagacgct ggcgtccttt ctgcatgaca cgcagctgct
    28081 gcagcgcagc ggagaaacgc cggatttcag caacacgctg ttcctgctcg atgagagctc
    28141 aatggtgggc aataccgaca tggcacgggc atacgccctg attgcggccg gtggtggtcg
    28201 tgccgtggcc agcggtgata cggaccagct gcaggccatc gcgcccggtc agcccttccg
    28261 tctccagcag acgcgcagtg ctgccgatgt ggccatcatg aaggagattg tgcgtcagac
    28321 gccggaactg cgggaggcgg tatacagcct gattaaccgg gatgtggaaa gggcactgtc
    28381 cgggcttgag agtgtgaaac cgtctcaggt tccccgtcag gagggcgcat gggcaccgga
    28441 gcactccgtg acggagttca gtcacagcca ggaagcgaaa ctggcagaag cgcagcagaa
    28501 ggcgatgctg aaaggcgagg ctttcccgga tatccccatg acactgtatg aagccattgt
    28561 ccgcgactat accggcagga caccggaagc acgggagcag acgctgattg tcacacacct
    28621 gaatgaggac cggcgcgtac tgaacagcat gattcatgat gcacgggaaa aggccggtga
    28681 gctggggaaa gagcaggtca tggtgcctgt cctgaacaca gcgaatatac gtgacggaga
    28741 actgcgtcgt ctctccacct gggagaataa cccggatgcc cttgccctgg tggatagtgt
    28801 gtatcaccgg attgccggta tcagcaagga tgacgggctg ataaccctgg aggatgcgga
    28861 aggtaacacg cggctgattt cgccccggga ggcggtggct gaaggcgtca cactgtacac
    28921 cccggacacc atccgggtgg gaaccggtga ccggatgcgc ttcacgaaga gtgaccggga
    28981 gcgcggttat gtggccaaca gcgtctggac ggtgacagcg gtttccggtg acagtgtcac
    29041 gctgtcggac ggacagcaga cccgggtgat tcgccccggt caggagcggg cagagcaaca
    29101 tattgacctg gcctatgcca tcaccgccca cggtgcgcag ggggcaagtg aaacctttgc
    29161 cattgcgctt gaaggcacgg aaggcgggcg gaaacagatg gccggctttg agtcggccta
    29221 cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc gtcagggctg
    29281 gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgc tggagccggg
    29341 gtctgaccgg gaggttatga atgcagagcg gctgttcagt acggcacggg aactgcggga
    29401 cgtggcggca ggccgcgccg ttcttcgtca ggcgggactt gccggggggg acagtccggc
    29461 acggtttatt gcacctgggc gcaaatatcc gcagccgtat gtggcactgc cggcgtttga
    29521 ccgtaacggc aagtcagccg gtatctggct gaacccgctg accacggatg acggaaacgg
    29581 gctgcgggga ttcagtggtg aaggccgggt gaaaggcagc ggggatgcgc agttcgtggc
    29641 cctgcagggc agccgtaacg gcgagagcct gctggcggat aacatgcagg atggcgtccg
    29701 gattgcccgt gataaccccg acagcggtgt ggtggtgaga atcgccggtg aaggccgtcc
    29761 gtggaatccc ggtgccatca ccggtggccg cgtgtggggg gatatcccgg acaacagcgt
    29821 ccagccggga gccggaaatg gcgaaccgat cacggcagag gtactggcac agcggcaggc
    29881 tgaagaggcc atccgccgtg aaacggaacg ccgtgcggat gaaattgtcc gtaaaatggc
    29941 agagaacaaa cctgacctgc cggacggcaa aacagagcag gctgtcaggg agattaccgg
    30001 gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg agagtgtact
    30061 gcgtgaacca caacgggaac gggaagcggt ccgcgaggtt gcccgggaaa acctgctgca
    30121 ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag agaaaaccct
    30181 gggcggagac tgatacagga agataaacgc tgatgacaac ggataacacg aacacgacac
    30241 gtaacgattc actggctgcc cggaccgata cctggttgca gtcgttgctg gtctggtcac
    30301 ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg gaccatgcaa
    30361 accgcattct gcatctggac cagacgtgga tgtttctggc cgggcgtgga gcctttccgc
    30421 tgtttgccct ggtgtggggg cggaatctgt cccgtcatgc gcatatccgg caggtggcca
    30481 ttaaccggct gtggggatgg gcagtgattg cccagttctc gtattatctg gccggctttc
    30541 cgtggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg ctgacgtggt
    30601 gtgagacgcg cagcttgtgg cgtactgctg cggccatact cctgatggca ctgtgggggc
    30661 ctttgtccgg caccagttac ggcattaccg ggctgctgat gctggcagtc agtcaccggc
    30721 tgtaccgggc ggaagacaga acggaacgtc tggcgctggt tgcatgcctg ctggccgtga
    30781 ttccggcact taaccttgcc tccagtgatg cggcggctgt agctggtctt gtgatgacgg
    30841 tgctgaccgt tggtctggtg tcgcgtgcag gaaaatcatt accccgtttc tggcccggag
    30901 attttttccc gacgttctat ggctgtcatc tggctgtgct gggcattctg gcgttgtgac
    30961 tggtatgata ctgacgctga ttctgcttat taataaacaa cataacacag gatatttatg
    31021 aaaattacgg atcacaaact ctctgaaggt attgccctga catttcgtgt tcctgaagga
    31081 aatataaaac atcctttgat tatcttatgc catgggtttt gcggtatcag gaatgttctg
    31141 ctcccttgct ttgcgaatgc cttcacagaa gcaggattcg caacgattac ttttgattat
    31201 cgaggattcg gtgagagtga tggagaacga gggcgtctgg ttcctgcaat gcagaccgaa
    31261 gatattattt cagttatcaa ttgggcagaa aagcaggaat gtattgataa tcagcgtatt
    31321 gggttatggg gaacatcgct gggagggggg catgtcttca gtgcagctgc gcaggaccaa
    31381 cgcgtaaaat gtattgtcag ccagctggct tttgctgacg gtgatgttct ggttaccgga
    31441 gagatgaatg aatcggagag agcgtctttt ctatccacgc tgaataaaat ggcagagaag
    31501 aagaaaaaca ccgggaaaga gatgtttgtg ggggttacac gggtactaag tgatgatgaa
    31561 tcgaaggtat tcttcgagaa aatcaaagcc cggcatcccg aaatggatat aaaaatacct
    31621 ttccttacgg tcatggaaac gttgcaatat aaaccggcag agagtgcagc cagcgtccag
    31681 tgcccagttt tggttgttat tgccggccag gatagcgtta atccaccgga acagggaaga
    31741 gcattatatg atgctgttgc ttctggtact aaggaattat acgaggaagc tgatgcatgc
    31801 cattatgata tttatgaggg cgcgtttttt gaacgtgtag tagctgtgca gacacaatgg
    31861 tttaaacaat atctttaatt gtcagtattt atacgttaaa ggtcgttgaa attaacttcc
    31921 tgttacgatg agttgtttca acggcctgca gggcatattt tcacgaaggt cagagtaagc
    31981 atggcagagc aaaaacgacc ggtactgaca ctgaagcgga aaacggaagg agagacgcct
    32041 gtccgcagcc ggaaaaccat catcaatgtc accacgccac caaaatggaa ggtgaaaaag
    32101 cagaagctgg ccgagaaagc agcccgggaa gcggagctgg cggcaaaaaa agcgcaggcc
    32161 agacaggcgc tgtccattta tctgaacctg cccacgctgg atgaggccgt gaacaccctg
    32221 aaaccctggt ggccgggatt atttgacggt gacacgcccc ggcttctggc ctgcggtatc
    32281 cgggacgtgt tactggaaga cgtggcacag cggaatatcc cgctctcgca taaaaagctg
    32341 cgcagggcgc tgaaggccat cacccgttca gaaagctatc tgtgtgccat gaaagccggt
    32401 gcctgccggt atgacacgga agggtatgtg acggagcata tttctcagga ggaggaagcg
    32461 tatgcggcag cgcgtctgga taaaatccgc cgccagaacc ggataaaggc agaacttcag
    32521 gccgtgcttg atgagaaata aaaaaagcct ccctcagaga gaggaggcag ggaaataagg
    32581 cttaaaagga aataacttcc gtacccgaaa ggaagccaat tcagtagatt atttaacacc
    32641 ataatactgg cgggttaaag aggagtgata aagatgaatg gattcagaaa cagttcacgg
    32701 aacggtcagg tctggcgtta ccagcgtgcc ggaagccggg ccgttattct ggaagtcagt
    32761 ggacgctgga tggaagcggc agaagcatgg cgacgggctg ccggtgttgc accccggaca
    32821 gactggcagc agtttgcccg aaaaagggct gaacactgcc accggcgctg ccggggaagg
    32881 gtgtaacgaa aaaagcagcc ttcattactc cagggattca gaaggctgaa taatgcatga
    32941 gatttgcttt ttttaatatc cggtcgggca gtatgaccgg acagttctga gtagcacaga
    33001 gttttttccg tattaagtcg tgattttata ttttgtgatt aatttcacaa aataaggtgt
    33061 tattcagtgt gtgctgcaat attcaggatg cctgaaatac aggtgctatt tatctgatac
    33121 ggagaataac atgaggaaat atattccact ggtattattt atcttttcat ggccggtatt
    33181 gtgtgcagat attcatggat gggttgttcg tgtactggac ggtgatacca tcgaggtcat
    33241 ggactcacgg aaggcggtgc ggatcaggct ggtaaatatt gatgcgccgg aaaagaaaca
    33301 ggattatggc cggtggtcga cagacatgat gaaatcactg gttgcaggaa aaacggttac
    33361 cgtcacatat tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcgccaga
    33421 cggaatgaat attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta
    33481 caacacagat cccgtgctgc ctgtccttca gaacgaagcc agacaacaaa aacgtgggct
    33541 ctggtcagat gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg
    33601 caatcctatc attcatttgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg
    33661 gctgtatcag ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa
    33721 cagagacagt ttatcgaaca gtgaacgtga atcatttaat tcagcagcag atcaccggtt
    33781 agcagaatta atcaccggaa agttatatga cagtatacca aaagaaatat ggaaatatgt
    33841 aaggtaataa ttcagcaata attaattacc aggagcagat aaacaatgaa gctgattatt
    33901 tttattctta ttgttctgat tattgcagcg ttacttatcc ggattatcct cagaagtgta
    33961 aatcagcatt ctcctttgct gatgcaattg catgcggcag gtatacggac cggtgatgca
    34021 gagcgaatac tgtccagcgg tgaatgctgg caacgtcaga agacgctgct gacagaaagg
    34081 gaagtcagtt ttatgaaagg actgttcaga attgtggata tgaagcggtg gtatctgtgt
    34141 ccgcaggtac gggtcgcgga tatcgtccag ctgaacggga atatccggcc acgatcacgc
    34201 cagtggtggc agttattcag gatggtgtct cagtggcatg ttgatgtggt tatcgttgag
    34261 cggcgttcgt tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg
    34321 gaacgcagac gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg
    34381 ctcagaagcc acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca
    34441 ggggctgatc agcagtcccc ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc
    34501 aaccccggaa acgggaaaaa gcaagttttc cccgctcccg gcgtttctat aactgaaaac
    34561 catactattt cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc
    34621 aacacagatc ttcgtcacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg
    34681 cgaaacgatc cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga
    34741 cttcctcatt gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga
    34801 aacaaatggc cgctgttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca
    34861 aaaatcctct gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc
    34921 agttcgttga gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac
    34981 tttactttgt ggcgtagcat gctagattac tgatcgttta aggaattttg tggctggcca
    35041 cgccgtaagg tggcaaggaa ctggttctga tgtggattta caggagccag aaaagcaaaa
    35101 accccgataa tcttcttcaa ctttggcgag tacgaaaaga ttaccggggc ccacttaaac
    35161 cgtatagcca acaattcagc tatgcgggga gtatagttat atgcccggaa aagttcaaga
    35221 cttctttctg tgctcgctcc ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc
    35281 accaaacgta ttaccgccag gtaaagaacc cgaatccggt gttcactccc cgtgaaggtg
    35341 ccggaacgct gaagttctgc gaaaaactga tggaaaaggc ggtgggcttc acctcccgtt
    35401 ttgatttcgc cattcatgtg gcgcatgccc gttcccgtgg tctgcgtcgg cgcatgccac
    35461 cggtgctgcg tcgacgggct attgatgcgc tgctgcaggg gctgtgtttt cactatgacc
    35521 cgctggccaa ccgcgtccag tgctccatca ctacgctggc cattgagtgc ggactggcga
    35581 cggagtctgc tgccggaaaa ctctccatca cccgggccac ccgagccctg acgttccttg
    35641 cagagctggg actgattacc taccagacgg aatatgatcc gcttatcggg tgctacattc
    35701 cgaccgatat cacgttcaca ccggcgctat ttgccgccct tgatgtgtct gaggatgcag
    35761 tggttgctgc gcgccgcagt cgtgttgaat gggaaaacag acagcgtaaa aagcagggac
    35821 tggataccct gggtatggat gaactgatag cgaaagcctg gcgttttgtg cgtgagcgtt
    35881 tccgcagtta ccagacagag cttaagtccc gtggaataaa gcgtgcccgt gcgcgtcgtg
    35941 atgcgaacag ggaacgtcag gatatcgtca ccctggtgaa acggcagctg acgcgtgaaa
    36001 tctcggaagg gcgcttcact gccaatcgtg aggcggtaaa acgcgaagtg gagcgtcgtg
    36061 tgaaagagcg catgattctg tcacgtaacc gtaattacag ccggctggcc acagcttccc
    36121 cctgaaagtg acctcctctg aataatccgg cccgcaccgg aggcatctgc acgcctgaag
    36181 cctgtcagcg aacaaaaaaa cagcaccgca tacaaaaaac aacctcatca tccaccttca
    36241 ggtgcatccg gtccctcctg tttttgatac aaaacacgcc tcacagacgg ggaaatttgc
    36301 ttatccacat ttaactacaa tggacttccc cataaggtta caaccgttca tgtcataaag
    36361 cgccagccgc cagtcttaca gggtgcaatg tatcttttaa acacctgttt atatctcctt
    36421 taaactactt aattaggggg cacctcagaa aacggaaaat aaagcacgct aaggcatagc
    36481 tgaccttgcc aggcctgctt cgccctgtag tgacgcgatc aacgggcagg aaacattccc
    36541 ctttcgtgca tggcaggcgc acacgagttc agacagcacg gtttccatgc gcgccaagtc
    36601 ggccatcttc tcgcgcacgt ccttgagctt gtgttcggcc aggctgctgg cctcctcgca
    36661 gtgggtgcca tcgtcgagcc gcaacagctc ggcaatctcg tccagactga accccagccg
    36721 ctgtgccgat ttcacgaatt tcacccgaac cacgtccgcc tccccatagc ggcggatgct
    36781 gccgtaaggc ttgtccggtt cccgcaacag gcccttgcgc tgatagaagc ggattgtctc
    36841 cacgttgacc ccggccgcct tggcaaaaac gccaatggtc aggttttcca aattattttc
    36901 catatcgctt gactccgtac atgagtacgg aagtaaggtt acgctatcca atccaaattc
    36961 aaaagggcca acgtatgtct gaaccacaaa acgggcgcgg tgcgctcttc gccggcgggc
    37021 tggccgccat tcttgcatcg acctgctgcc tggggccgct agtactggtc gccctgggct
    37081 tctccggtgc ttggatcggc aacctgacgg tgctggaacc ctatcgaccg ttgttcatcg
    37141 gcgcggcgct agtggcgctg ttcttcgcct ggaagcggat ttaccggccc gtgcaggcat
    37201 gcaagccagg tgaggtctgc gcgattccgc aggtgcgcgc cacctacaag ctgattttct
    37261 ggatcgtggc cgtgctggtc ctggtcgcgc ttggatttcc ctatgtcgtt ccatttttct
    37321 attaaccagg agttcatcat gaagaaactg tttgcctccc ttgccctcgc cgccgctgtt
    37381 gccccggtgt gggccgctac ccagaccgtc acgctagcgg ttcccggcat gacttgcgcc
    37441 gcctgcccga tcacagtcaa gaaagcgctc tccaaggtcg aaggcgtgag caaggtcgat
    37501 gtgggcttcg agaagcgcga ggccgtcgtc acttttgacg acaccaaggc cagcgtacag
    37561 aagctgacca aggccaccgc agacgccggc tatccgtcca gcgtcaagca gtgagccagc
    37621 aagccaacga caacagcgag agccgcttca tgggactgat gacacgcatt gccgataaaa
    37681 ccggcgcgct cggcagcgtc gtttccgcga tgggctgcgc cgcctgcttt ccagccctcg
    37741 ccagcttcgg cgcggccatc gggctgggct tcttgagcca gtacgaggga ctgttcatca
    37801 gccgcctgct gccgctgttt gccgcgctgg ccttcctggc gaacgcgctg ggttggttca
    37861 gtcatcggca atggctgcgc agtctgctcg gcatgatcgg cccggccatc gtgtttgcgg
    37921 ccacggtctg gctgctcggc aactggtgga cggcgaacct gatgtacgtc ggcctggcct
    37981 tgatgattgg ggtgtcgatc tgggacttcg tgtcgccggc gcatcgccgt tgcggaccgg
    38041 acggctgcga actccccgcc aagcgcttgt gaaagacggc tgaccgtgcg acacggcggc
    38101 ccacacgaat aaggaacgat ggtatgagca ctctcaaaat caccggcatg acttgcgact
    38161 cgtgcgcagt gcatgtcaag gacgccctgg agaaagtgcc cggcgtgcaa tcagcggatg
    38221 tctcctacgc caagggcagc gccaagctcg ccattgaggt cggcacgtca cccgacgcgc
    38281 tgacggccgc tgtagctgga ctcggttatc gggccacgct ggccgatgcc ccctcagttt
    38341 cgacgccggg cggattgctc gacaagatgc gcgatctgct gggcagaaac gacaagacgg
    38401 gtagcagcgg cgcattgcat atcgccgtca tcggcagcgg cggggccgcg atggcagcgg
    38461 cgctgaaggc cgtcgagcaa ggcgcacgtg tcacgctgat cgagcgcggc accatcggcg
    38521 gcacctgcgt caatgtcggt tgtgtgccgt ccaagatcat gatccgcgcc gcccatatcg
    38581 cccatctgcg ccgggaaagc ccgttcgatg gcggcatcgc cgctaccacg ccgaccatcc
    38641 agcgcacggc gctgctggcc cagcagcagg cccgcgtcga tgaactgcgc cacgccaagt
    38701 acgaaggcat cttggagggc aatccggcga tcactgtgct gcacggctcc gcccgcttta
    38761 aggacaatcg caacctgatc gtgcaactca acgacggcgg cgagcgcgtg gtggcattcg
    38821 accgctgcct gatcgccacc ggcgcgagcc cggccgtgcc gccgattccc ggcctgaaag
    38881 acactccgta ctggacttcc actgaagcgc tggtcagcga gacgattcct aagcgcctgg
    38941 ccgtgattgg ctcatcagtg gtggcgctgg agctggcgca ggcgttcgcc cgactcggag
    39001 cgaaggtgac gatcctggct cgcagcacgc tgttcttccg cgaagaccca gctataggcg
    39061 aagccgtcac ggccgcattc cgcatggagg gcatcgaggt gagggaacac acccaggcca
    39121 gccaggtcgc gtatatcaat ggtgaagggg acggcgaatt cgtgctcacc acggcgcacg
    39181 gcgaactgcg cgccgacaag ctgctggtcg ccaccggccg cgcgcccaac acacgcaagc
    39241 tggcactgga tgcgacgggc gtcacgctca ccccgcaagg cgctatcgtc atcgaccccg
    39301 gcatgcgtac aagcgtggaa cacatctacg ccgcaggcga ctgcaccgac cagccgcagt
    39361 tcgtctatgt ggcggcagcg gccggcactc gcgccgcgat caacatgacc ggcggtgacg
    39421 cggccctgaa cctgaccgcg atgccggccg tggtgttcac cgacccgcaa gtggcgaccg
    39481 taggctacag cgaggcggaa gcgcaccatg acggcatcaa aactgatagt cgcacgctaa
    39541 cgctggacaa cgtgccgcgc gcgctcgcca acttcgacac gcgcggcttc atcaaactgg
    39601 tggttgaaga aggcagcgga cgactgatcg gcgtgcaggc agtggccccg gaagcgggcg
    39661 aactgatcca gacggccgca ctggcgattc gcaaccggat gacggtgcag gaactggccg
    39721 accagttgtt cccctacctg acgatggtcg aagggttgaa gctcgcggcg cagaccttca
    39781 acaaggatgt gaagcagctt tcctgctgcg ccgggtgagg acaaggaggt gtgcgatgag
    39841 cgcctacacg gtatcgcaac tggcccataa cgctggggtg agcgtacata tcgtgcgcga
    39901 ctacctggtg cgcggcttgt tacggccggt ggcctgcacc acgggcggct acggcgtgtt
    39961 cgacgatgcg gccttgcaac ggctgtgctt cgtgcgcgcg gccttcgagg cgggtatcgg
    40021 cctggatgcc ctggcgcggc tgtgccgtgc gctcgacgca gcggacggcg cacaagccgc
    40081 agcgcagctt gccgtgctgc gccagttggt cgagcggcgg cgcgcggcgt tggcccatct
    40141 ggacgcgcaa ctggcctcca tgccagccga gcgggcgcac gaggaggcat tgccgtgaac
    40201 gcccctgaca aactgccgcc cgagacgcgc caacccgttt ccggctacct gtggggtgcg
    40261 ctggccgtgt tgacctgccc ctgccatctg ccgattctcg ccgccgtgct ggccgggacg
    40321 accgccggtg ccttccttgg cgagcattgg ggtgttgccg cgctcgcgct gaccggcttg
    40381 ttcgttctgg ccgtaacgcg gctgctgcgc gccttccggg gcggatcatg acgagttcgc
    40441 agcccgccgg atggacggcg gccgagttgg cgcaggcggc ggcgcgcgga cagcttgacc
    40501 tgcattacca gccgctggtc gatctgcgcg atcaccggat cgctggcgcg gaagcgttga
    40561 tgcgctggcg gcatccgagg cttggcctgt tgccgcccgg ccagttcctg ccgctggccg
    40621 agtcgttcgg cctgatgccg gaaataggcg cgtgggtgct gggcgaggcc tgtcgccaga
    40681 tgcacaagtg gcaaggaccg gcatggcaac cgttccgtct tgccatcaat gtgtccgcca
    40741 gccaggttgg gccaacgttc gacgacgagg taaagcgggt gctggccgat atggccctgc
    40801 ccgccgagct tctggagatc gaactgaccg aatcggtcgc attcggcaat ccagccctgt
    40861 tcgccagttt cgacgccttg cgcgccatcg gcgtgcgctt cgccgccgac gacttcggca
    40921 ccggctattc ctgcctgcaa catctgaaat gctgccccat caccacattg aaaatcgacc
    40981 aatcctttgt cgccaggctc ccggatgatg cccgtgacca aactatcgtg cgggcggtga
    41041 tccagctcgc gcacgggctg ggcatggatg tcattttcag aagacgactg caccagttga
    41101 ttgggcgtaa tggctgttgt gcagccagct cctgacagtt caatatcaga agtgatctgc
    41161 accaatctcg actatgctca atactcgtgt gcaccaaagc gaggtgagca tggcgacgga
    41221 caccccacgg attccagaac aaggcgtggc cactctgcct gatgaggctt gggagcgtgc
    41281 gcgccgtcgt gcggagatca tcagtccgtt ggcgcagtcg gagacggtcg ggcacgaagc
    41341 ggccgatatg gcggctcagg cgctgggctt gtctcggcgc caggtatacg ttctgatccg
    41401 gcgtgcccgg caaggcagcg gcctcgtgac ggatctggtg cccggccagt ccggtggagg
    41461 taaaggtaag gggcgcttgc cggaaccggt cgagcgcgtc atccacgagc tactgcaaaa
    41521 gcggttcctg accaagcaga agcgcagcct agcggccttt caccgcgaag tcactcaggt
    41581 gtgcaaggct caaaaactgc gagtgccggc gcgcaatacc gtggccttac ggatcgctag
    41641 ccttgacccg cgcaaggtca tccgccggcg ggaaggccag gatgccgctc gtgacctaca
    41701 aggtgtgggc ggcgagcctc ctgccgtgac cgcgccgctg gagcaggtgc agatagacca
    41761 tacggtcatc gacctgatcg tggtcgatga ccgcgaccgg caacctattg gccgcccgta
    41821 cctgaccctc gccatcgacg tgttcacccg ctgcgtgctc ggcatggtcg tcacgctgga
    41881 agcgccgtct gccgtttcgg ttggcctgtg cctcgtgcat gtcgcctgcg acaagcgccc
    41941 ttggctggaa ggactgaacg tggaaatgga ttggcagatg agcggcaagc ccttgctgct
    42001 ctacctagac aacgcggccg agttcaagag cgaggccctg cgccggggtt gcgagcagca
    42061 tggcatccgg ctggactatc gcccgctggg acagccgcac tatggcggca tcgtggaacg
    42121 gatcatcggc acggcgatgc agatgattca cgacgaactg ccgggaacga ccttctccaa
    42181 ccctgaccag cgcggcgact acgattccga aaacaaggcc gccctgacgc tgcgcgagct
    42241 agagcgctgg ctcacattgg cggtcggcac ctaccacggt tcggtgcaca acggcctgct
    42301 ccaaccgccg gccgcgcgct gggccgaggc cgtggcgcgt gtcggcgtac cggccgtcgt
    42361 cacacgcgct acttcgttcc tggtcgattt tctgccgatc ctccggcgca cgctgacccg
    42421 caccggcttt gtcatcgacc acatccacta ctacgccgat
//
LOCUS       DAZFZC010000035        42335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFZC010000035 DAZFZC010000000
VERSION     DAZFZC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.035"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..527)
                     /gene="espX5"
                     /locus_tag="ACHKXP_002153"
     CDS             complement(<1..527)
                     /gene="espX5"
                     /locus_tag="ACHKXP_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO7647158.1"
                     /translation="MCYNGLNNMFFPLCQINDNHSVTSPSHTKKTKSDNYRKHHKSTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIASHANNK
                     CDDRITRRVYLNKDKEVSIKVVYFINNVTVHNNTIEIPQTVNGGYDFSHLSLKDIVIK
                     DEDLSNSNFAGCRLQ"
     gene            complement(705..2354)
                     /gene="actP"
                     /locus_tag="ACHKXP_002154"
     CDS             complement(705..2354)
                     /gene="actP"
                     /locus_tag="ACHKXP_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO7647159.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2351..2665)
                     /gene="yjcH"
                     /locus_tag="ACHKXP_002155"
     CDS             complement(2351..2665)
                     /gene="yjcH"
                     /locus_tag="ACHKXP_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO7647160.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2866..4824)
                     /gene="acs"
                     /locus_tag="ACHKXP_002156"
     CDS             complement(2866..4824)
                     /gene="acs"
                     /locus_tag="ACHKXP_002156"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO7647161.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            5217..6653
                     /gene="nrfA"
                     /locus_tag="ACHKXP_002157"
     CDS             5217..6653
                     /gene="nrfA"
                     /locus_tag="ACHKXP_002157"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO7647162.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6698..7264
                     /gene="nrfB"
                     /locus_tag="ACHKXP_002158"
     CDS             6698..7264
                     /gene="nrfB"
                     /locus_tag="ACHKXP_002158"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO7647163.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            7261..7932
                     /gene="nrfC"
                     /locus_tag="ACHKXP_002159"
     CDS             7261..7932
                     /gene="nrfC"
                     /locus_tag="ACHKXP_002159"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO7647164.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7929..8885
                     /gene="nrfD"
                     /locus_tag="ACHKXP_002160"
     CDS             7929..8885
                     /gene="nrfD"
                     /locus_tag="ACHKXP_002160"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO7647165.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8905..10623
                     /gene="nrfE"
                     /locus_tag="ACHKXP_002161"
     CDS             8905..10623
                     /gene="nrfE"
                     /locus_tag="ACHKXP_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO7647166.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRSEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGVVVSSVSRQEISLN
                     LQPGQPVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGLDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10616..10999
                     /gene="nrfF"
                     /locus_tag="ACHKXP_002162"
     CDS             10616..10999
                     /gene="nrfF"
                     /locus_tag="ACHKXP_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO7647167.1"
                     /translation="MSKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10996..11592
                     /gene="nrfG"
                     /locus_tag="ACHKXP_002163"
     CDS             10996..11592
                     /gene="nrfG"
                     /locus_tag="ACHKXP_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO7647168.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     HGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11934..13247
                     /gene="gltP"
                     /locus_tag="ACHKXP_002164"
     CDS             11934..13247
                     /gene="gltP"
                     /locus_tag="ACHKXP_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO7647169.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13551..14240)
                     /gene="yjcO"
                     /locus_tag="ACHKXP_002165"
     CDS             complement(13551..14240)
                     /gene="yjcO"
                     /locus_tag="ACHKXP_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO7647170.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(14334..16481)
                     /gene="fdhF"
                     /locus_tag="ACHKXP_002166"
     CDS             complement(14334..16481)
                     /gene="fdhF"
                     /locus_tag="ACHKXP_002166"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(16062..16064),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO7647171.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16679..18145)
                     /gene="mdtP"
                     /locus_tag="ACHKXP_002167"
     CDS             complement(16679..18145)
                     /gene="mdtP"
                     /locus_tag="ACHKXP_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO7647172.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYSMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLATGVAQLYYSMQASYQMLDLLEQTHDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIVAVKGQITETRESLRALIGAGASDMPE
                     IRPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLHTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAGPVVEKK"
     gene            complement(18142..20193)
                     /gene="mdtO"
                     /locus_tag="ACHKXP_002168"
     CDS             complement(18142..20193)
                     /gene="mdtO"
                     /locus_tag="ACHKXP_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO7647173.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQNAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(20193..21224)
                     /gene="mdtN"
                     /locus_tag="ACHKXP_002169"
     CDS             complement(20193..21224)
                     /gene="mdtN"
                     /locus_tag="ACHKXP_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO7647174.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(21243..21518)
                     /gene="ytcA"
                     /locus_tag="ACHKXP_002170"
     CDS             complement(21243..21518)
                     /gene="ytcA"
                     /locus_tag="ACHKXP_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO7647175.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(21727..23712)
                     /gene="yjcS"
                     /locus_tag="ACHKXP_002171"
     CDS             complement(21727..23712)
                     /gene="yjcS"
                     /locus_tag="ACHKXP_002171"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO7647176.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINATAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMGNGDNLNLTLNDSVLNYRKTLQPQANASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(23985..24914)
                     /gene="alsK"
                     /locus_tag="ACHKXP_002172"
     CDS             complement(23985..24914)
                     /gene="alsK"
                     /locus_tag="ACHKXP_002172"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO7647177.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLRDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLVAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQFCAKAP"
     gene            complement(24898..25593)
                     /gene="alsE"
                     /locus_tag="ACHKXP_002173"
     CDS             complement(24898..25593)
                     /gene="alsE"
                     /locus_tag="ACHKXP_002173"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO7647178.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(25604..26584)
                     /gene="alsC"
                     /locus_tag="ACHKXP_002174"
     CDS             complement(25604..26584)
                     /gene="alsC"
                     /locus_tag="ACHKXP_002174"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO7647179.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(26563..28095)
                     /gene="alsA"
                     /locus_tag="ACHKXP_002175"
     CDS             complement(26563..28095)
                     /gene="alsA"
                     /locus_tag="ACHKXP_002175"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO7647180.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVEKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(28222..29157)
                     /gene="alsB"
                     /locus_tag="ACHKXP_002176"
     CDS             complement(28222..29157)
                     /gene="alsB"
                     /locus_tag="ACHKXP_002176"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO7647181.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(29216..30106)
                     /gene="alsR"
                     /locus_tag="ACHKXP_002177"
     CDS             complement(29216..30106)
                     /gene="alsR"
                     /locus_tag="ACHKXP_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO7647182.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            30465..30914
                     /gene="rpiB"
                     /locus_tag="ACHKXP_002178"
                     /gene_synonym="alsI"
     CDS             30465..30914
                     /gene="rpiB"
                     /locus_tag="ACHKXP_002178"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO7647183.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            30983..31312
                     /gene="yjdP"
                     /locus_tag="ACHKXP_002179"
                     /pseudo
     CDS             30983..31312
                     /gene="yjdP"
                     /locus_tag="ACHKXP_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
     gene            complement(31355..32113)
                     /gene="phnP"
                     /locus_tag="ACHKXP_002180"
     CDS             complement(31355..32113)
                     /gene="phnP"
                     /locus_tag="ACHKXP_002180"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO7647184.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTQKFLLNNHPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(32115..32549)
                     /gene="phnO"
                     /locus_tag="ACHKXP_002181"
     CDS             complement(32115..32549)
                     /gene="phnO"
                     /locus_tag="ACHKXP_002181"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO7647185.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(32536..33093)
                     /gene="phnN"
                     /locus_tag="ACHKXP_002182"
     CDS             complement(32536..33093)
                     /gene="phnN"
                     /locus_tag="ACHKXP_002182"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO7647186.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(33093..34229)
                     /gene="phnM"
                     /locus_tag="ACHKXP_002183"
     CDS             complement(33093..34229)
                     /gene="phnM"
                     /locus_tag="ACHKXP_002183"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO7647187.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQNGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(34226..34906)
                     /gene="phnL"
                     /locus_tag="ACHKXP_002184"
     CDS             complement(34226..34906)
                     /gene="phnL"
                     /locus_tag="ACHKXP_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO7647188.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(35017..35445)
                     /locus_tag="ACHKXP_002185"
                     /pseudo
     CDS             complement(35017..35445)
                     /locus_tag="ACHKXP_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(35443..36303)
                     /gene="phnI"
                     /locus_tag="ACHKXP_002186"
                     /pseudo
     CDS             complement(35443..36303)
                     /gene="phnI"
                     /locus_tag="ACHKXP_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
     gene            complement(36303..36887)
                     /gene="phnH"
                     /locus_tag="ACHKXP_002187"
     CDS             complement(36303..36887)
                     /gene="phnH"
                     /locus_tag="ACHKXP_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO7647189.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSTPLNNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(36884..37336)
                     /gene="phnG"
                     /locus_tag="ACHKXP_002188"
     CDS             complement(36884..37336)
                     /gene="phnG"
                     /locus_tag="ACHKXP_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO7647190.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVQGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(37337..38062)
                     /gene="phnF"
                     /locus_tag="ACHKXP_002189"
     CDS             complement(37337..38062)
                     /gene="phnF"
                     /locus_tag="ACHKXP_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO7647191.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(38083..38870)
                     /gene="phnE"
                     /locus_tag="ACHKXP_002190"
                     /pseudo
     CDS             complement(38083..38870)
                     /gene="phnE"
                     /locus_tag="ACHKXP_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
     gene            complement(38976..39992)
                     /gene="phnD"
                     /locus_tag="ACHKXP_002191"
     CDS             complement(38976..39992)
                     /gene="phnD"
                     /locus_tag="ACHKXP_002191"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7647192.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(40017..40805)
                     /gene="phnC"
                     /locus_tag="ACHKXP_002192"
     CDS             complement(40017..40805)
                     /gene="phnC"
                     /locus_tag="ACHKXP_002192"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO7647193.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(40938..41381)
                     /gene="yjdN"
                     /locus_tag="ACHKXP_002193"
     CDS             complement(40938..41381)
                     /gene="yjdN"
                     /locus_tag="ACHKXP_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO7647194.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(41839..42174)
                     /gene="yjdM"
                     /locus_tag="ACHKXP_002194"
     CDS             complement(41839..42174)
                     /gene="yjdM"
                     /locus_tag="ACHKXP_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO7647195.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         9432 a        11205 c        11594 g        10104 t
ORIGIN      
        1 ttttgtagtc tgcaacctgc aaaattcgaa ttggataaat cttcatcttt aatcacgata
       61 tctttcaggc taaggtgtga aaaatcgtaa ccaccattta ctgtctgtgg gatttcgata
      121 gtattattat ggacggtgac attatttata aaatatacca ccttaattga cacttcctta
      181 tctttattaa ggtaaactct tctcgtaatt ctatcgtcac atttattatt tgcatgtgaa
      241 gctatatcta taaaatattt atgtattttc ctgtccgttt ccttcgtgat ttttcctgat
      301 ggtaaactat cataaattct tttcattttc tgaatcaagc ctatcacttt ttcatgatca
      361 tccatcttga aaagagagag ggctttattg tcaattaacg tgcttttatg atgttttctg
      421 taattatctg attttgtttt ctttgtatgt gatggacttg tgacagagtg gttatcgtta
      481 atctggcaaa gagggaaaaa catattattt aaaccattgt agcacataaa atatccttat
      541 aattaacaag aaagaaaagg catattctct gcattatcat tttctacgac tgtcaaaaat
      601 cgctcatttt ttaatgagtt tatttgttta atattttggg aaaaggtgat gcatgctgga
      661 gaggaagagt attccccggt caggcgaccg gggaagggga gagattaatg tgcgcggcct
      721 tgctcaacgc caaagccggt ctgggagcgg ataaactgcg cgcggaacag ttcacgctcg
      781 cgcgcccctt ccgctgagtt atcggttgcc gagaagaacc agatgccgag gaatgccacg
      841 gtgatagaga acagcgccgg gtattcatac gggaagatgg ctttttcgtg accaaggatc
      901 tgtacccaaa tcgtcgggcc gaggatcatc agtactactg cggtaatcag ccccagccag
      961 ccacccatca tcgcgccacg cgtggtcagt ttcgaccagt acatagaaag cagaatgatc
     1021 gggaagttac agctcgccgc gatggcaaac gccagcccca ccataaaggc gatgttctga
     1081 ttctcaaaca gcacgccgag gataatcgca atcacgccga ggatcagtac ggtgattttt
     1141 gatacccgca gctcttcacg ttcggtcgcg ccttttttga agacgttagc gtacaagtca
     1201 tgcgaaaccg cggatgcgcc cgccagcgtc agacccgcaa ccaccgcgag gatagtggcg
     1261 aaagcaaccg ctgaaataaa accgaggaac aggttgccgc ccactgcatt cgccaggtga
     1321 acggccgcca tgttgttacc accaatcaga tggcccgccg cgtctttata ttccggattc
     1381 gcaccaacca gcatgatcgc gccgaagccg ataataaagg tcagaatata gaagtagccc
     1441 ataaacccgg tggcgtagaa cacgctctta cgtgcttcgc gggcatcgct gactgtaaag
     1501 aagcgcatca gaatgtgcgg caagcccgcc gtaccaaaca tcagtcccag acccagagag
     1561 agcgcggaga tcggatcttt caccagcccg cccggcttca tgatgtcgac ccctttcggg
     1621 tgtaccgcca tcgcttcgct gaacagattg ttgaagctaa agccaacgtg tttcatcacc
     1681 ataaaggcca taaagctggc accgaacagt aacagcacgg ctttaataat ttgcacccag
     1741 gtggtcgcca gcatgccgcc gaacaggaca tacatcatca tcagcacgcc gaccagcacc
     1801 accgcaatgt gatagttaag gccaaacagc agctcgatca gtttacctgc gcctaccatc
     1861 tgagcgataa ggtaaagcgc caccaccacc agagaaccac aggccgaaag aatacgaatc
     1921 ggcccttgtt tcaggcggta agaggctaca tcggcaaagg tgtagcgtcc caggttacgc
     1981 agacgttcgg caatcaggaa caaaatgatc ggccagccca ccaggaagcc cagcgagtaa
     2041 attaagccgt catagccgga ggtaaacacc agtgcggaga tccccaaaaa tgaggcggcg
     2101 gacatatagt caccggcaat cgccagcccg ttctggaagc ctgtgatatt gccgcctgcc
     2161 gtgtagtagt cgctacgaga acgtacgcgt tttgatgccc agtaagtaat gccgagcgta
     2221 aacacgacga aaatcaggaa cataataatc gcctgccagt tcgttggctg gcgctctacg
     2281 gccccgctaa tagcatccgc ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc
     2341 agaactcttt tcatgatgct tgtacctcat gcaggacttc gttattaaga cggtcgaatt
     2401 cgccgttcgc ccgccagatg tagataccgg tgagaacaaa ggagatcaca atcactccaa
     2461 caccaatcgg aataccgcgt gtgacgctgg tgttcggatt cagcggggtg cccagccagc
     2521 cgggcgcgaa ggcgatcagt aaaataaagc cgatataaac tgccagcata ataatcgaca
     2581 ggatggtggc aaaccgttgc cgtttttcga ctaactccct gaaatgcgca ttgtcttcta
     2641 tccgctgata aatcgtacca ttcatcacag attctccaga ggtaatgtag ggattgtttt
     2701 aattcccgct cccttatggg agaaggttaa cgctcgggta acccttgccg aatgtaggcc
     2761 ggataaggcg ttatacgccg catccggcaa tcaatgcctg atgcgacgct gtcgcgtctt
     2821 atcaggccta caaaccgtta ccgactcgca tccggcaatt gtgggttacg atggcatcgc
     2881 gatagcctgc ttctcttcaa gcagcttctc gactacgcca ggatcggcaa gcgtcgaggt
     2941 atcgcccagg ttgctggtat cgcccgccgc aattttgcgc agaatacggc gcataatttt
     3001 gccggagcgg gttttaggca gggagtcggt ccagtgcagc acgtctggcg tcgccagcgg
     3061 gccaatctct ttacgcaccc agttgcggac ttctgcgtac agttctggtg acggttcctc
     3121 cccgtgatta agcgtgacgt aggcgtagat cgcctgacct ttaatattgt gcggaatacc
     3181 tactacggcg gcttcggcaa tcttcggatg cgccaccagc gccgactcaa tctccgccgt
     3241 tcccagacgg tgaccggaga cgttcagcac atcgtccaca cgcccggtta tccagtaata
     3301 gccatcttca tcgcgacgcg cgccgtcgcc gctgaaatac atatttttga aggtggagaa
     3361 gtaggtctgt tcaaaacgtt cgtgatcgcc aaacagcgta cgcgcctgac ccggccagga
     3421 gtcggtgatc accaggctgc cttcggtagc gccttccagc gggttacctt cgttatcgac
     3481 cagcgccggt tgcacgccga agaacggacg tgttgccgaa ccggctttca gctcggtagc
     3541 gccaggcagc ggcgtgatca tgaaaccgcc agtttcggtc tgccaccagg tatcgaccac
     3601 cggacatttc tcgttgccga tttttttcca gtaccactcc cacgcttccg ggttgattgg
     3661 ctcgcccacg gaaccgagaa tgcgcagcga cgatcggtcg gtgccttcaa tcgctttatc
     3721 cccttccgcc atcagcgcgc gaatcgccgt gggcgcggta tagagaatat tgacctgatg
     3781 cttgtccacc acctgcgcca tacgggcagg cgtcggccag ttcggtacgc cttcaaacat
     3841 cagcgtggtc gcaccgcagg ccagcgggcc gtacagcaag taactgtgtc cggtcaccca
     3901 gcccacatcg gcggtgcacc agtagatatc acccggatga taatcaaaga catatttaaa
     3961 ggtcagcgcc gcgtacacca gataaccgcc ggtagtatgc agcacacctt ttggcttacc
     4021 ggtagaaccg gaggtgtaga gaataaacag cggatcttcg gcgttcatct cttccgcctg
     4081 gtgctgatcg ctcgcttgct caaccaggtc gtgccaccac aggtcgcgcc cttcctgcca
     4141 gtcaattttc ccgccagtac gcttcagtac caccacatgc tctacgctgg tgacgttcgg
     4201 gtttttcagc gcgtcatcaa cgtttttctt cagcggaata ctgcgcccgg cacgcacacc
     4261 ttcgtcggaa gtgatcacca gtcgtgagtt ggaatcaata atgcgcccgg caacggcttc
     4321 cggcgagaag ccgccgaaaa tcaccgaatg caccgcgcca atgcgggcgc aggccagcat
     4381 cgcaaccgcg gcttccggca ccatcggcat ataaatcgcc accacatcac cttttttaat
     4441 gcccagctcg agcagggtat tggcgaagcg gcagacgtcg cggtgcagct ctttatagct
     4501 gatatgtttg ctctggctgg cgtcgtcgcc ttcccagatg atggcggtac gatcgccgtt
     4561 ttcttgcaga tggcggtcaa ggcagtttgc cgccagattc agcgtgccgt cctcgtacca
     4621 tttaatggac acattaccgg gggcaaagga ggtgtttttc accttctggt aaggtttgat
     4681 ccagtcaaga atttttccct gttcgcccca gaaggtatca ggtacgttaa tagattgttg
     4741 atacatcgcc tcgtactgct gagggtttat caggcaacgg tctgcgatgt tggcaggaat
     4801 ggtgtgtttg tgaatttggc tcatgctttt gttctccttg taggatgtta ataatatgtg
     4861 gcataagcgt taaatgtagg ggtattggca gttttgttta gtatttgggc gacagatcac
     4921 gcaaaagtag aattgtgcaa ataaacggca gggtaatttt tgaaggtcag caacaaaagt
     4981 tgattaattc tttgaggaac atgcagttat gcatgctgtt gaaaaagagg aagatactga
     5041 ctaactctaa agtggtattt tacatgcact tacaattgat taaagacaac attcacagtg
     5101 tggttatttg ttacacatag gggcgagcaa tgtcatgaca gtgtaggtgc ggttactgtc
     5161 gtgaaaaaca ataaaaaccg ccattgcaac aatggcgcaa ttcggatgaa gcccctatga
     5221 caaggataaa aataaacgca cgccgtatct tcagcttatt gattcctttt ttctttttca
     5281 cttctgttca cgctgaacaa acggctgctc ccgcaaaacc tgtaactgtg gaagcgaaga
     5341 atgaaacctt tgccccgcag catcccgatc aatatctctc ctggaaagcc acctcggaac
     5401 agtcagagcg tgttgacgcc ctggcggaag atccacggct ggtgatcctg tgggcggggt
     5461 atcccttctc gcgcgattac aacaagccgc gtggacatgc ttttgctgtg accgatgtgc
     5521 gtgaaaccct gcgtaccggt gcgccgaaaa acgctgaaga tggtccgcta ccgatggcat
     5581 gctggagttg taaaagcccg gatgtggcgc gtctgatcca gaaagacggc gaagatggct
     5641 acttccacgg taaatgggcg cgcggcggtc cggaaatcgt caacaactta ggttgtgccg
     5701 attgccataa caccgcctct ccagagttcg ccaaaggcaa accggagtta accctttccc
     5761 gtccgtatgc ggctcgcgcg atggaagcca ttggtaaacc ttttgagaaa gccggacgtt
     5821 tcgaccagca atcgatggtt tgcggtcagt gccatgtgga gtattacttc gacggcaaaa
     5881 acaaagcggt taaattcccg tgggatgacg gcatgaaagt cgaaaatatg gagcagtatt
     5941 acgacaaaat tgccttctct gactggacta actccctgtc gaaaacgcca atgctgaaag
     6001 cgcagcaccc ggaatatgaa acctggacag cgggcattca cggtaaaaac aacgtgacct
     6061 gtatcgactg ccatatgcca aaagtgcaga acgccgaagg caaactctac accgaccata
     6121 aaattggtaa tccgtttgat aacttcgccc agacttgtgc gaactgccat acccaggaca
     6181 aagctgcctt gcaaaaagtg gtcgcggaac gtaagcagtc gattaacgac ctgaaaatca
     6241 aggttgaaga tcaactggtt cacgctcact tcgaagcgaa agcagcgctg gatgcaggcg
     6301 cgacggaagc tgaaatgaag ccaattcagg acgatatccg tcatgcccag tggcgctggg
     6361 atctggcgat cgcttcccac ggcattcata tgcacgcacc ggaagaaggt ttacggatgc
     6421 tcggtacggc gatggataaa gcggcggatg cacgcaccaa actggcgcgc ctgctggcga
     6481 ccaaaggcat cacccatgaa atccagatcc cggatatctc aaccaaagag aaagcccagc
     6541 aggccattgg cctgaacatg gaacaaatca aggccgagaa gcaggacttc atcaaaacgg
     6601 tgatcccgca gtgggaagaa caggcacgta aaaacggtct gttaagccaa taaccccgtt
     6661 ccgcctcgca aggggcggaa aacacaatgg agtgaatatg agcgtattac gttcgttatt
     6721 aactgccggg gtgctggcgt cgggcctgtt gtggagcctg aacggaatta ccgctacccc
     6781 tgcggcgcag gcatctgatg atcgctacga agttacccag cagcgtaacc cggatgccgc
     6841 ctgtctggac tgtcataaac cagataccga aggtatgcat ggaaaacatg cttccgttat
     6901 caacccgaat aacaaactgc cggtcacctg caccaactgt cacggccagc catcaccgca
     6961 acaccgcgaa ggggtgaaag atgtgatgcg ctttaacgag ccgatgtaca aggttggaga
     7021 gcagaacagc gtctgtatgt cctgtcatct gccggaacag ttgcaaaaag cgttctggcc
     7081 gcacgatgtc cacgtaacca aagtggcgtg cgccagctgc cattccctgc atccacagca
     7141 agatacgatg cagacgttaa gtgacaaagg acggattaag atttgcgtcg attgccacag
     7201 cgatcagcgc accaatccga actttaaccc ggcgtctgtt ccgttgctta aggagcagcc
     7261 atgacctggt ctcgtcgcca gtttctcacc ggcgtcggcg tgctggcagc cgtcagcggc
     7321 accgcagggc gcgtggtggc gaaaacgttg aatatcaatg gggtgcgtta cggcatggtg
     7381 catgacgagt cgttatgcat cggctgtacc gcctgtatgg atgcttgtcg ggaagtgaac
     7441 aaggtgccgg aaggtgtctc gcgcctgacg attattcgta gcgagccgca gggcgaattt
     7501 cctgatgtga aatatcgctt cttccgtaag tcttgccagc actgcgatca tgcgccgtgc
     7561 gttgacgtct gcccgaccgg tgcgtctttt cgcgatgctg ccagtgggat tgtcgatgtt
     7621 aatccggatc tctgcgtcgg ttgtcagtac tgcatcgccg cctgtccgta ccgcgtgcgc
     7681 tttatccatc cggtcacgaa gacggcggac aaatgcgatt tctgccgtaa gaccaatttg
     7741 caggccggta agttgcccgc gtgcgttgaa gcttgcccga ccaaggcgct gacgtttggc
     7801 aatctggacg atcccaacag tgagatttcg caactgctgc gccagaagcc cacttaccgc
     7861 tacaagctgg cgctgggaac caaaccgaag ctgtaccgcg taccgtttaa atacggggag
     7921 gtgagccaat gacgcagact tccgcatttc attttgaatc gctggtgtgg gactggccga
     7981 ttgccatcta cctgtttttg attggtattt ctgctggtct ggtgacgctg gccgtgctgt
     8041 tacgtcgctt ctacccgcag gcgggcggtg cagacagtac gttgctgcgc accacgctga
     8101 ttgtcgggcc gggcgcggtg atcctcggtc tgttgatcct cgtcttccac ctgacaagac
     8161 cgtggacctt ctggaagctg atgttccact acagttttac ctcggtgatg tcgatggggg
     8221 tgatgctgtt tcagctctac atggtggtgc tggtgctgtg gctggcgaaa atctttgaac
     8281 atgatttgct tgccctgcaa caacgctggt tgccgaagct ggggatcgtg caaaaggttc
     8341 tgagcctgct gacgcccgtt catcgcggac tggaaacatt gatgctggtg ctggcggtgt
     8401 tgttgggggc ttataccggc tttctgctgt cggcgctgaa atcgtatccg ttcctcaata
     8461 acccgatcct gccggtgctg ttcctcttct ccggcatctc gtccggtgcg gcggtggcgc
     8521 tgatcgccat ggcgatacgc caacgcagta acccgcattc cacggaagcg cagtttgtac
     8581 accgtatgga aatccctgtg gtatggggtg aaatcttcct gctggtggcg ttttttgtcg
     8641 gtctggcgct gggcgatgac ggtaaagttc gtgcgctggt ggcggcatta ggtggcggtt
     8701 tctggacgtg gtggttctgg cttggtgtcg ccgggctggg gctgattgtg ccaatgttgc
     8761 tcaaaccgtg ggtcaatcgc agttccggca ttcctgccgt gctagcggcg tgtggggcca
     8821 gtctggtcgg cgtgttgatg ctgcgctttt tcattctcta cgccggacag ttgacggtgg
     8881 cgtaagccag aaaagaggtg gtttctggac gtattccttc ctgaagtcgg ttttctggcg
     8941 ttgttgttaa gtctcggggt caacgtgttg accccgttga cggcctttgc gggagtgcgg
     9001 ttgcgctggc ctgccatgat gcgactcact tgcatcggca ttctggcgca gttcgcgctc
     9061 ctgctgctcg cctttggcgt actgacgtat tgttttctca tcagcgattt ctcggtcatt
     9121 tatgtcgcgc aacatagcta cagcctgctg tcgtgggaac tcaagctggc agcggtgtgg
     9181 ggcggccatg aaggttcgct gctgctttgg gtgctgctgc tttccgcctg gagcgcgctg
     9241 tttgcctggc attatcggca gcaaaccgat ccgctatttc cgctgacgct agccgtttta
     9301 tctctcatgc tcgccgcact gctactgttt gtggtgctgt ggtccgatcc cttcgtgcgg
     9361 atatttccac cagcaatcga aggccgcgat ctcaatccga tgctgcaaca tcccggtctt
     9421 atctttcatc caccgctgct ttatctcggg tatggcggtt tgatggtggc ggcgagcgtg
     9481 gcgctggcga gtttactgcg cagcgagttt gatggtgcct gcgcccgaat ttgctggcgc
     9541 tgggcactac ctggctggag cgcattaacg gcggggatca ttctcggttc ctggtgggcc
     9601 tattgcgaac tgggctgggg cggctggtgg ttctgggatc cggtggaaaa cgcctcttta
     9661 ttaccctggc tttctgccac tgcgctgctg cacagtttat ctctgacacg tcagcggggg
     9721 attttccgcc actggtcgct gctgctggcg atagttactc tgatgctgtc gctgctgggc
     9781 accttaattg tccgttctgg cattctggtt tcggttcatg cgttcgcgct ggataacgtc
     9841 cgcgccgtgc cgttgttcag cctgtttgca ctgattagcc ttgcgtctct ggctctgtat
     9901 ggctggcgag cgcgggacgg tggcccggcg gtgcgttttt cggggttatc gcgggaaatg
     9961 ttaatcctcg ctacgctgtt gctgttttgc gcagtgctac tgatcgtgct ggtgggaacg
    10021 ctttatccga tgatttacgg cctgctgggc tggggacgcc tctccgttgg cgcaccttat
    10081 tttaaccgcg cgacgttacc gtttggtctg ttgatgctgg tggtgattgt gctggcgacg
    10141 tttgtctctg gcaaacgcgc gcagcttccg gcgctggtag ctcatgcggg cgtgctgtta
    10201 tttgccgctg gggtcgtggt ttccagcgtc agccgtcagg aaatcagtct gaatttacag
    10261 ccgggtcagc cggtgacgct ggcaggatac accttccgtt ttgagcgcct cgatctgcaa
    10321 gccaaaggca attacaccag cgaaaaagcg atagtggcgc tgtttgacca tcagcaacgc
    10381 attggtgaat taacgccgga gcggcgtttt tacgaagctc gtcgtcagca aatgatggaa
    10441 ccgtcaattc gctggaacgg catccatgac tggtatgcgg tcatggggga gaaaactggg
    10501 ctggatcgtt acgcttttcg tttgtatgta caaagcggtg tgcgctggat ctggggggga
    10561 ggattgttga tgattgcagg cgcattatta agcggatggc gggggaggaa gcgcgatgag
    10621 taaaggactt ctcacgctac tgctgttatt tacctgtttt gcgcgcgctc aggttgtaga
    10681 cacctggcaa ttcgccaatc cgcaacaaca ggaacaggcg ttaaatattg ccagccagtt
    10741 acgttgtccg cagtgccaga atcaaaactt actggaatcc aatgcgccgg tggcagtcag
    10801 tatgcgccat caggtttaca gcatggtggc ggaggggaag aacgaagtcg aaatcattgg
    10861 ctggatgacc gaacgctacg gagattttgt tcgctataac ccaccgttaa cgggtcagac
    10921 gctggtgtta tgggcgctgc cagtggtgtt gttactactg atggcactga tcctctggcg
    10981 agtgagggcg aagcgatgaa acagcccaaa ataccggtga aaatgctgac aacactcaca
    11041 atattgatgg tatttctctg tgtcggcagt tatctgttaa gcccaaaatg gcaggcggta
    11101 cgtgcggagt atcagcgtca gcgcgatccg ctacatcagt ttgccagcca gcaaaccccg
    11161 gaagcgcagc ttcaggcatt acaggataaa atccgcgcta atccacaaaa cagcgaacag
    11221 tgggcgttac tgggcgagta ctatctgtgg caaaacgatt acagcaattc gctgctggcg
    11281 tatcgtcagg cgttgcaact acatggtgag aacgctgaac tgtatgcggc gctggcgacg
    11341 gtgctttatt accaggccag ccagcatatg accgcccaga ctcgcgcaat gatcgacaaa
    11401 gccctcgcgc tggactctaa cgaaatcacc gctctgatgc tgctggcttc cgatgcgttt
    11461 atgcaggcga attacgcgca agccatcgaa ctatggcaaa aagtgatgga tctcaactca
    11521 ccccggatca accggacaca gctggtcgag tcgattaata tggcgaaact gttgcagcgg
    11581 agatcggatt aatcatctgg tgatgaagat tatgtcattg agctgcataa aaaataatcg
    11641 aatgaacata tgccaaaaat aatcactaat cagtattatt gcagattaaa caaataaaaa
    11701 tctttccata acaaatggtt attcattaat cctgccataa actcgcgtta ttatgcatta
    11761 atgcagcgaa aagctctgtt gttaaagggt tgcgcaacat accgcgcaaa tgatactgat
    11821 cataagcgtt aaaaaaatct acaaaccaac gcaacacaat tcatgccctg gcagtatgtc
    11881 acgttctcgc gtttctgaac ggggaacggc gctccattga ggaagtcatt catatgaaaa
    11941 atataaaatt cagcctggcc tggcagattc tgtttgctat ggtgctgggc attctcctgg
    12001 gaagctacct gcactaccat agcgacagcc gcgactggct ggtcgtcaat ttgctctctc
    12061 cggcgggtga tatcttcatc catctgatca aaatgattgt tgtgccgatt gtgatctcca
    12121 cgctggtggt gggtatcgcg ggtgttggtg atgccaaaca gctcgggcgt attggcgcga
    12181 aaaccattat ttacttcgag gtgatcacca ccgtcgccat cattttgggg atcactctgg
    12241 cgaacgtctt ccagcccggt gccggggtgg atatgtcgca gctggcgacc gtcgatatct
    12301 cgaaatatca gagcactacg gaagcggtac aaagcagttc ccacggcatt atgggcacga
    12361 ttttgtcgct ggtgccgacg aacattgtgg cgtcgatggc gaaaggcgaa atgctgccga
    12421 tcatcttctt ctcggtgctg tttggtctgg ggctttcctc cctgcccgcg acgcatcgtg
    12481 aaccgctggt gaccgtgttc cgctccatct ctgaaaccat gtttaaagtg actcacatgg
    12541 tgatgcgtta tgcaccggtg ggtgtgtttg cgctgattgc ggtgacggtg gctaactttg
    12601 gtttctcttc tctgtggcca ctggcgaaac tggtgctgct ggtgcatttc gccattctgt
    12661 tcttcgcgct ggtagtgctg ggaattgtgg cgcgcctgtg cgggttaagc gtctggatcc
    12721 tgattcgtat tctgaaagat gagctgattc tggcgtactc cactgccagc tctgaaagcg
    12781 tactgccgcg aattattgag aagatggaag cctacggagc accggcgtcg atcaccagct
    12841 tcgtggtgcc gaccggttac tcttttaacc ttgatggttc gacgctgtat caaagtattg
    12901 ccgctatttt catcgctcag ctgtatggca ttgacctgtc catctggcag gaaatcattc
    12961 tggtgctgac gctgatggtg acctcgaaag ggattgctgg cgtgccggga gtgtcgtttg
    13021 tggtgttgct ggcaacgctg ggtagcgtag gtattccgct ggaaggtctg gcgtttattg
    13081 ccggtgttga ccgtatcctc gacatggcgc gtactgcgct gaacgttgtg ggtaatgcgc
    13141 tggcggtgct ggtgattgcc aagtgggaac acaaatttga ccgcaaaaag gcgctggctt
    13201 atgagcgtga agtgctgggt aaatttgata aaactgcgga tcaataattg aagattgccg
    13261 gggatatcca cccggcaatg tgtggatgcc tgatgcgacg ctggtgcgtc ttatcaggcc
    13321 tacgccagac agcgcaatag cctgatttag cgtgattttg taggtcggat aaggcgttta
    13381 tgccgcatcc gacatcaacg cctgatgcga cgcttaacgc gtcttatcag gcctacgcca
    13441 gacagcgcaa tagcctgatt tagcgtgatt ttgtaggtcg gataaggcgt ttacgccgca
    13501 tccgacatca atgcctgatg cgacgcttgc cgcgtcttat caggcctatc ttaaccgttg
    13561 gttaattttt caaactcttc acacccggta tcaaaccctt ccatacagct caggttcaac
    13621 cagtgcagcg ccttttgctt gttcttctcg ataaaacctt cttcaccgtt taagaacatc
    13681 attcccgccc agtactcgga ataaccggtt cgggaaattg cagagctgcg tttgaaatac
    13741 caggttgcct tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat caaaccaagc
    13801 agcatttggg catcgacggc agagtcgttc tccagatctt ccgaggcgtt ttctaacagc
    13861 gaaattgcct ttggataatc cggtttaccc gcttgagtat ttaccagaat atgcgccagc
    13921 gttacttcac cttctttact gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt
    13981 ggatagtcca gactgaccgg attggtgatt ttgatctgcg ccagcagcgc gcaggcatca
    14041 gcatcaccgc tgtcggcggc tttctgtgcc caatattcgg ctttgctcaa atcgccggag
    14101 ctaaaccagc tgtcggcaag aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt
    14161 aaatactggc tgccaggctc ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc
    14221 aatgcgataa tttttttcat tttgataata cgattgggta gggagtaacc agtataaaga
    14281 gttcgaatgg ataaaaaaac agcctccgaa aggaggctgt agaaaggacg gtattacgcc
    14341 agtgccgctt cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac gtactgctcg
    14401 gcggcgcgct gatcggcgat cggctcgacg cgcacggcgc agtatttgta ttccggcgtt
    14461 ttcgtaatcg ggcttaagtt ttcggtaacc agctcgttac aggcaccaat ccaccactgg
    14521 taggtcatgt aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg ggtgataatt
    14581 ttgcctttac gcgagtgcac ccaaaccaat gcctcatctt caatacccag acgtttggcg
    14641 tcttcggtat tgatttgtgc gtagccaggt tcatcagcca gcgccgccag tgccgcacag
    14701 ttaccggtca tcgaacggca agagtagtga ccaacttcac gcaccgttga cagtaccatc
    14761 gggtactcgt cggtgagttt gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc
    14821 agaccgttcg gggtatcaaa cttctcttta aacagataag aagtcccctg atcggcatct
    14881 gaagtatcgc ggcaaggcca ctgaatgaag cccagttcgc ccattttctc gtaagtcgca
    14941 ccgtagaaat ccgggcacag atgacgcaac tcatcccaga tctcctgggt gttgttgtag
    15001 tgcatcggat aacccatacg ggtggcgatt tcactgatga tttgccagtc cgttttcaga
    15061 tcccatttcg gttcaaccgc cttgaagaaa cgctggaagc cacggtcagc cgcagtaaac
    15121 acgccttcat gctcgcccca cgacgttgac ggtaaaataa catccgccgc cgacgcggtt
    15181 ttggtcataa agatgtcctg aacgataacc agttccagat cttcaaaggc tttacgtact
    15241 gccgacagct ccgcgtcagt ttgcagcgga tcttcgccca taatgtacgc ggcacgcact
    15301 tcgccatgcg ctgcgcggtg cggtagctcg ctgatgcgat agccggtatg cgctggcagg
    15361 ctttccacgc cccaggcttt ggcgaatttc tcgcggttag ccggatcttt cacgtactgg
    15421 tatcccggat acgtatccgg cagcgcgccc atatcgcagg caccctgtac gttgttctga
    15481 ccacgaaccg ggttaacacc cgcatgtggc ttaccgaggt taccggtcag catcgcgagg
    15541 ctggtcagag aacgcacggt ttccacgccc tggtagaact gggttacacc catgccccac
    15601 aggatggcgg cgcttttcgc ctgggcatac atccgtgccg cctgacgaat ctcactggcg
    15661 ctgacgccgg tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat tttacgatac
    15721 tcttcaaagc cttctgtacg ggaagcgacg aatgctttgt cgtacaaatt ttcttcaata
    15781 atgacatggc ccatcgcatt caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc
    15841 atgtcagcaa tgcgcgcggt ttcaattttg cgcggatcgc agacgataat tttcgccccg
    15901 ttacgtttag cgttaattac gtgattcgct acgattgggt gggaatccgc cgggttgtac
    15961 ccgaaaacga acactaaatc ggtattatca atttcgttaa tagcattgct cattgcgcca
    16021 ttaccgaccg attggtgcag acctgcaacc gatgggccgt gtcagacacg agcgcagcag
    16081 tcaacgttat tggtaccaat aacggcgcgc gcaaattttt gcattacata gttggtttcg
    16141 ttacccgtac cacgcgagga gccggtcgtc tggatggcat ccggaccgta cttctctttg
    16201 atggcgctca ggcgctcggc aacgtaattc agtgcctcat cccaggaaac aggttcgagt
    16261 ttgccgccac gctggcgacg gatcatgggg gttttcaggc gcggggtcag gatctgggta
    16321 tcgttaatga agtcccagcc ataataaccc ttcagacaca gggtaccctg gttggttttc
    16381 ccctgcgctg cctccgcccg gacgattttg ccgttatcga cgaccaggtt gattttgcaa
    16441 cctgatgcgc aataggggca aaccgtgacg acttttttca tcggtctcgc tccagttaat
    16501 caaatcacgc atacgcgctc tcgactacag tatgcatctt ttatgccaca ttttatgtgg
    16561 ggtcattccc tgatattacg ggcactattt attcaaaact ctgacgaaaa acaggctgtc
    16621 gtcagttttg acgtgacgaa acgaaatacc gcgtgacagc catcacgcgg cagacatttt
    16681 attttttctc gacgacggga cctgcctgat acccgccgcc cagcgatttc atcaactgaa
    16741 tgctttggat cacccggcgg ctgtccagca tcagtaatga catctcttcg gcaagcactg
    16801 gcaaccgggc ttcggtggcc tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg
    16861 cagcgcgctg ggtaaagcgc gtggcttcca cgcgttcagc ctgcatttct cgctcgtcgt
    16921 tgagcgtttg cagacgcgtg ccgttgacgg caacgtcacg caccgcgttc agtactgact
    16981 ggttgtaacg ttcaatcatc atgttgctgg cggcgcgcgt gccttcgaga ttggcattca
    17041 accgtccacc gtcaaacagc ggcaatttca gacccgggat gaagttgaac tggcgactgg
    17101 tttttttgaa taaggtatgc agatggatgg agtccagacc gaaaaacgct ttgatatcaa
    17161 agctcggata gaacaacgcc cgcgcggaat ccacctgatc taatgacgcc tgaacatacc
    17221 agcgcatggc ttgcagatcc gggcgtctgg cgagcaactc ataagagagt gtcgccggaa
    17281 tgccggtctg gacttgcggt aatgccaccg gtctgatctc cggcatatcg ctggctcccg
    17341 cgccaatcaa tgcacgcaga gattctcgcg tttcggtgat ttgccctttg acggcaacaa
    17401 tttgtttatc gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact tgcgcttcca
    17461 gaccgtgcgc cactttactc tggtgcgctt tcaccgcgta atcaatcaca tcgtgagttt
    17521 gttctaacag atcgagcatc tgatagctgg cctgcatact gtaataaagc tgcgctacgc
    17581 ccgtggccag cgatagctct actgctgcgg tttctgccag cgcggcatta tgcgcgccaa
    17641 tggcggcggc aaccgctgag cgatgcacac cccacaaatc aagatccagt ccggcaaaca
    17701 aacctactgt ggcttccgta tagtacggcc cgtccatacc cagtgctggc gcatccattg
    17761 aataagggct taaaaagccg ttcgccgaga cacgttggcg gttgagcatc cctaacgctg
    17821 cgacctgtaa ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct ttttcttccc
    17881 gcagtttcgc ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca
    17941 gctgcgggtc attgagttgt ttccaccact gcgcctgcgg ccagccggag ctggcaagat
    18001 gaatatcgtc ggccagtttg atttgttccg gtttgagctg ttgatgaggt gcagaatcct
    18061 tacgtaccag ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca
    18121 gacgtgaaag ttgacgattg atcattgcgt ggctccttgt gcctgttccg tggcgggcgt
    18181 taatgccggg gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg
    18241 ttttaataag gtgggcagga tggcctgaga ggcgggtgtc tcttcaagcg ttatttgagg
    18301 agaacggctg agtgcggttg ccagaccggc agcgtatttc tccagggcgt cggcaaactg
    18361 acctgctctg tccggctgta gtgcgttatc cagcgcctgc gccgagttcc aggtggcatc
    18421 ccaggcgtga agaagatcgc ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa
    18481 tgcgcgttct tcgctgtcca gttgacgctc cagcgccacg cgttggcaca tctcttcaca
    18541 ggcattaaac gccgcatgca gaccgatacg aatttgcaga taagtgcgca gagccgtgac
    18601 ttcctgctgg cgtgggatcc gcattacttt acttaacata cccagcgtgc cagccagttt
    18661 ttgcggcagt gtgcgcgctt cactttcagg ccagacaaag gtgtaaatca ccgcggacac
    18721 cacggtacca atgatgatac ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg
    18781 gccaaaaacg ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg tgccgatata
    18841 agaagagcgt tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac
    18901 aaacagcaat tcgacaatat tgtccagcca gggcatgacc agtagcgtga ataacagcgc
    18961 cagaatcgcg ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt acgacgaacc
    19021 gacatttggg ttagcgacga tcacgcatgt cagcatacag gtgtgaatgc cttcccagtc
    19081 cacgccgctg taaaaggtgt aacagatcaa acaggcgagc agcgttttta ccgcgtagcg
    19141 catatagtct ggattggtaa aagcatcggc ggccattgat ggcggtttgg ctgcgggcgt
    19201 tggcggcgta ttcgggtcca tctgacccag ttgtaacaac gtctggcaga tattctccag
    19261 gttacattcc cgtgccgcca tcgcttcact ttcactgatc cgccagtcgc tttgccagca
    19321 ctgaccttca gcaacggcat gctgcagctt gttgatttct gaagctaatt tttgtcggaa
    19381 ttcaataatt gcctgagaat cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta
    19441 aatgtaggtt accgttgcca cgcagctttg ccaccatgcg ttttgagttc gccagttggc
    19501 atcgtccgcg aggcaaaaga cattgagttt ttgtagcgcc agcgcctctc tttcaatccg
    19561 cgtttcgggt agcggtgcga ggctgtctgt caggtgacta atggcatcat caagccgatc
    19621 attaagcgcc tgatgcattt gcgaaatggc acgactggga aaccacagca cgccgattaa
    19681 cgtcatcagc aaggttggat agaggccaac aacgatacac cacagcgtta agcgcacgac
    19741 cacttccgga tagtcgagca tggcggggaa ggtttgcccg taaatagcga caatggcgac
    19801 ggcgaaaaag accagcccca agcgatgggt gcgcatcaaa aacatgcagc ccatcaggat
    19861 cggtccggcg atgatcaatc ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa
    19921 caggctgccg atctccagca ccgtggcaac cacaaacaag atcgcgacaa atttggtgta
    19981 aaacgcgttc gactgaatac cgtaaaacag cactgccagc gataacgcca caaaagggat
    20041 ctcaaaggtc atcgagatca gaatcaccag caggcagcct acccagagtt gcacggtctg
    20101 cggcacgcga cctggtcgcc gctcgcttaa ctcttcatga aagaacgcca gcagcctgac
    20161 caccggtaat ggcagggagt tgagcgcgct cattattgcg gctcaagatt agcgactgcc
    20221 gaagcgccga tgcggaacat ttccgggtca ggtttatcga ccatgatttt gaccggaaaa
    20281 cgctgggcaa cgcggaccca gttaatagaa cgagacactt tcggcaggcc gcccagcacc
    20341 aggccgccgt catccggtag cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt
    20401 ttgccgctgt cactcatcag gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga
    20461 tcggtttcgc ggaagttggc gatcacatac cagtgacgag tgtcgattag ggtaaaaata
    20521 gggcgcatgg cagaagcaaa ttgcccgacg gaggttttga gggaaatgac ccggccatca
    20581 aacggcgcgc gaacggtcgc catttccaga tgcagtttgg tcagggcaat atccgcttcg
    20641 accgccgcac gctgggcaac taatgcatcc acgccgctga cggcgctggc ggctgactgc
    20701 gcctgtaaca atacggcatt aagatccgct tctgcggcgc gctgcgccgt tcttgcacgg
    20761 tcaacatctt ccgctgagac aaaaccttct ttcagtaatg gctcggtgcg gcgtaatgta
    20821 tctgtggcct gtttcgcggc ggcacgggct ttttctaccg tggcattaac cgagtcggca
    20881 ccaaactgtt gcgcgtcaac gctacgctgg gtgagcataa tttgcttatc cagcgccgcg
    20941 agggaggctt cagctttcgc cagattggct tcgtacgggc gcgggtcgat gcggaacagc
    21001 aaatcgccct gtttgactgc ctggttgtcg gtgaccgcca gttctacaat gcggccgctg
    21061 acttccggta ccacatcaat ggtatctgct gacgcgtaag cgtcattagt tgatggcgca
    21121 ctgtctacgc gccagataac aaaaacaagg gcaaccagcg ccaacgcaac cactaacaga
    21181 gcagggaatt tactgcgagg agcttttttc ggcgtacttt ccatgagcgc gtctcaaata
    21241 gattagaaaa atgccagcca gaacagcatg gcgtagagag caaaaagggc ggtataaata
    21301 attccgacaa atgccagatt gatatttgcc cgctgaataa ctcgcctcgt gatgagcgtc
    21361 aaaataaggc tggcaatggc gcagaaaaac cagctgggat aataagcgcc gatcaccggg
    21421 attgccggag ataatgagca tcccgaaacc atgaagacgg gaattatcca ggctgttttt
    21481 ttgagttgca tcgccatgcg agagagaact gttggcatat taaatacatc ctgtctgctg
    21541 cgaacaatat ctgtagtgaa tatcaatatc cattgaaggc gtcagtatgg ctattttctt
    21601 gatgaataaa aatagcttta ttcagccacc gcattcataa cttatatatt ttatgtatta
    21661 attggttgta tatattttta aaataaatgc ttttgggggc ttcagcgccc ccaaaagcga
    21721 gtggcattaa tttggggtta cgatattcac ccacaaatcg aaattatcca gacaggcaat
    21781 aatttcttcc agtttcgcgc cattgccaat aattttggct ttcttcgctt ttaccagatc
    21841 cgccattttt gcctgtccgg tcagcacggc gtgcagatct tcacggctga tgtagaaaga
    21901 ggcgttggct tgcggttgca gcgttttccg gtagttaagc acactatcgt tcagcgtcag
    21961 gttgaggtta tcgccgttgc ccatattgaa gttcaggctg atatttttac ccgcggcttt
    22021 cgcgctatcg aggcgaacgg acataaagtc gaacagcatt tcgaccgaca tcccgcgaat
    22081 ggtgtccggg gaaccggtgg tgccgtggct gaacttatgc accccttcgc gcagctcttt
    22141 cgcgccggtc aggtagaaac cgcgccaggt ggcggactcg gcctgatagc ccagctgttc
    22201 aaagttattc gcttgcagat tcttcgcgac ctggtcaccc gggttggcgg caatcacctg
    22261 tttcagcagt tctgccgacc agcggtaatc accttgcttg ttcgcttctt gcgccaggtt
    22321 gatgacacgg gcagaaccgc ccagcgcctg cacgtaacgt ttacccatct ccacctgacc
    22381 atacggatgc aggttagccg ggttaccgtc gtaatagcca agatagaagt tatacaccgc
    22441 gcgggcgttg tggctgacag aaccgtaata gccacggctg gcccagttat tggcaagtgc
    22501 aggcggcagc ttaatcatgt cgccgatttc attcatggtg tagccctggt tcgccaggtg
    22561 cagggtctgg tcgtgaatgt acttgatagt atcgcgatat ttaccaatat aatcattgat
    22621 atgcttattg ccccagaccg gccaggtgtg cggcataaac aggacttccg cgtcgttacc
    22681 ccacatatcc agcgtttcgt tcagatactc ggtccacttg ctggtgtcgc gggttttcgc
    22741 gccgcgtaga gtgtagaagt tgtgcagggt atgcgtggcg ttctcggcgg tacacagggc
    22801 tttcagggcc ggaatataga agtgcatttc ggctggcgct tcgctacctg gggtcatcag
    22861 gaagtcaaac tccaggccgt cgataatcat cttctcgcca gttcggacga tagttttagt
    22921 cggtgcaata atgctcgggt cgcccgttgc cagcgtcacg ccaaggccat tacccacgtt
    22981 gccttgcgcg ttgtgcggca gtaacagacc gtaagagtag agcgcacggc ggctcatgat
    23041 gttacccgcc agcacgtttt cgctgatggc ttcgtccata aagcctgcag gggcaatcac
    23101 ctgaactttg ccggatttaa catcggcttc agagataatg cctttcacgc caccatagtg
    23161 gtcggtgtgg ctgtgagtgt agataacggc aacaatcggt ttttgcggac gatgctggaa
    23221 gtaaaggtca agtgcggctt ttgcggcagg cggcgtcacc agcgggtcga taacaataat
    23281 gcctttctcg ccctcaacga acgtaatgtt agagatatcc tggccgcgca cctgatacat
    23341 tttatcggtg actttgaaca ggccagaaat accgttgatt tgcgactgac gccacaggct
    23401 ggggtttacg gtttccggcg ctgtggcatt aatatcaaat ttgtaatcgt ccgcgcggta
    23461 gtaaacttta ccgttcgcat cacgcagaat accttcatcc agcagcgggg cgataaatcc
    23521 acgctgggca tcgtcaaaat cctgacgatc gctaaacggt aatgatttgg cgtaattctg
    23581 attaacctgt tgggtatatt gagtggcggc tttcgcttcc tctttcgcgt tagcggtatt
    23641 taccatcatg ccgctggcgg cagttaacgc aataacaatc ctgctcaaac ggaataaccg
    23701 agagttattc atataaatct ccatttatca tttatgtcgt aaatatgtaa tccatgcaaa
    23761 atacgtcatt taaagctggc gttatcataa ttgggaaatt gcgactttaa taagtggaag
    23821 tgtgagcgga acgcgccatt ttattaggca tttgtgctga agagggaaaa ttacagtaaa
    23881 actatcattg cagaaaataa tatgagtcgt tttatgtgtt tattatatat attgtacctg
    23941 tcgttggcac agcgggacat tgcgctactg tgccaacgtt ttactcatgg ggctttagca
    24001 cagaactgtg gcaaaaaacg ttgatgcgcc aatattgctg caccctgagc gccattaaag
    24061 tcagaagatg aggcggcaat aaagcgcacg acctgatgcg gcagtggacg gcgcaggtac
    24121 ttttgggtca tggcaacgag agtctcgcgt gggaaggcgg gcatatccat cacgccaccg
    24181 cccaggatca ccgcatcggg atcgaacaga ttaatgctgg tggcaatggc ccgtgccgcg
    24241 ttttcaagca gactctggac gaaaggggcg ttttccgcat ggacgaaaag atcgcgcaat
    24301 gggtaatttc ggggctgttg ttcgtaccag cgtcttagcg ccattccaga gcaattggtt
    24361 tccaggcacc caggattgcc acacgcgcag tgttgggtca tatctcccag ggggatatga
    24421 cccagttcgc ctgccacacc gtgtgcaccc gtccacggcg caccgttcat ccacactgcg
    24481 aaccccatcc ccgtaccgag ataggccgcc agaaccagtt gttgcgtaag gcggttttct
    24541 actacgtccc aggagagttg caggttaacg tcgcgggaaa actcaaccgg acaattcagc
    24601 gtattttcga gcttatcggc gagatcatat aaatccgccg ctgttaacgg caggttaggc
    24661 gtagaaataa tggtgcgttt atctttactg accagcgccg gaaatcccat caccagacca
    24721 tgacagcgag cgttaaagcg cctgagttgc tcgtcaatca tttcgccgat acccgacacc
    24781 aggccgggag caatgacttc tgcggtccgc tttttttcgc agtgtagcgt ttcaccttct
    24841 gctgtccgca gacaaaagcg gatatgcgtt gcccccatat ccacgcccgc tacgacgtta
    24901 tgctgttttt gcatgaggct gtacctcgct ttttgcagcc agaatctgcg cggtcataat
    24961 tctccatgct tcgtcgatat tttccgcatg attaaacagg ccggaagtgc cgacgataaa
    25021 gacatccgcc cctgccgcca tcagtttttc gtaagttgcc tggttgcagg aaccgtccac
    25081 ctcaatttcg tactccagac cttctcgttc acgccatgcc ttcagttcgg caagtttatc
    25141 cagcatttca ggaatgaacg gttgtccggc aaagccggga tcgacagtca tgaccgtaat
    25201 tttatcggcc ttatggatat agtatttcat ggcctcaact ggcgtctccg ggttaaggat
    25261 cagccccact ttcatgtcat gacggcggat ttcatcaatc aggcggaacg cctggccgtt
    25321 gatggtttcc ggatgcagag tgatgaaatc tgctcccgca cgcgccagtt gagcaatgta
    25381 atcctgcggc cgcgtcacca tcagatgaca gtcgagcggt ttagttgcca gttttttaac
    25441 ctgacttacg aagaacggtg agagtgtcag attggggaca aagtgaccgt ccatgatatc
    25501 gatgtggaag taatcggcat ggctgtcgat aaattcgatc tgttctttaa atttcagcag
    25561 atccatacac attaacgagg gggagatttt catgttcaat tccttactta ctgataagac
    25621 ggtcaagggc gacagccgcg ataattaatc cgcccatcac caccagttgg taataggttt
    25681 gtacctgcaa aatattcaga ccgttgttga tggtgccgat gatcaacccg ccaatcacca
    25741 cagagaaaat gcgccccttg ccgccgaaga aactggtgcc gccaatgatg gcgctggcaa
    25801 tggcataggt ttcaaaaccc ataccggcaa gcggttctgc ggcaccgagt cgtgcagttg
    25861 agacgacgcc tgccagacct gcacaaacac cggagatgat aaacaccacc aggatgtgga
    25921 atttcacgtc aatcccggaa tagaacgccg aatttttgtt gccgcccagt gcgtagatgt
    25981 tgcgcccgag ccgcatacgc gttgtcagaa accaaaggat gagcgcgaca attagtgaga
    26041 agataacggg gacaggtatc ccaattacgc tggcggcaaa gaagttcacg aagtcaaatg
    26101 agaagccgta taccgagttg gcatcggaga tcaccagcgt gatcccacgg aaaatcgcgt
    26161 tggtgccaag ggtgatgatg aacgggtgta gccccgtcca gttgaccagg cagccgttga
    26221 tcgcccccag tgcgccgcca accagtacac cgccaatcat cgctgcgaga aacgggtcaa
    26281 cacctgccaa catcagtttg gcggtcacca taccggaaag cgccagaatc gcgccaaccg
    26341 agaggtcgat accagcgacc aggatagcga aaaactcgcc catgccgatc aataccgtca
    26401 cggagctttg aacaaaaatc tgggtaatat tattggtggt cagaaaatat tctggtgaca
    26461 gcgaaccaaa gatggcgacg atgatcgcca ggataaaaaa ggtgccgtat ttatcccaga
    26521 acagcgcaaa gttgaacggt ttcttctcgc tcgcttcgct ttttactctt gtggtaaagc
    26581 ccatgccata atctcctctt cgctcatgtc atcgcgattc gtcaggattt gcgtcagtcg
    26641 tccttcgcag aacacggcga tgcggtcgca gacggtgata atttcaggta gttcagatga
    26701 caccatcagg atgacttttc cgtcgtccgc cagttggcgc atcactttgt aaatttcggc
    26761 tttcgcgcca acgtcgatgc cgcgggtagg ttcatcgaaa atgatcactt ccgggcaaca
    26821 gcacagccat ttggagatca gtactttctg ctgatttccc ccggagagtt cggtgatatt
    26881 ctggtttacc gaatgacatt tcagcgccag cagttcgcgt tgattttcag cggtacgttg
    26941 ctcgtcaact tcatgaaaca agcccatcgc gcctttatag ccgccgtctt tcagactgcg
    27001 gctgatcgcc atgttctgag cgatggaaaa gttggggaaa aaaccgttat cccggcggct
    27061 ttcagtgatg taagccatcc cttttttcac ggcatccagg ggggaacgtg gagagatatc
    27121 tttgccatta agacggattt ctccgccagc gcgtttctca acgccaaaca ggcaattcat
    27181 cagttcagta cgtccggaac cgaccagccc ggcaaagcct aatatttctc cccggcagac
    27241 gctaaatgag atatcccgga ccttttttct gtcacggctg gtgacgttcc gcacctcaaa
    27301 aaccgtatcg tgcgcaaggt tgctgacatt ctccttcatc gcgttaaaac ggttttgcag
    27361 ttcgcggcct accatcagac ggacgatatc gtcatttgac acatcgctca ccatgccgct
    27421 gcaaacgctg ctgccgtctt tcatcaccgt atagcggtcg caaatacggc gaatttccgc
    27481 caacttatgc gagatataga cgatggccgt accctcttta cgcaactgat tcatgatcag
    27541 aaacagatag tccacctctt tattggtgag tgaggaggtg ggttcatcca tgatgatgac
    27601 tttggcatcg agcatcagcg ttttggcaat ttccagcatc tgcttgtggc tgatagataa
    27661 attcgccact ttctcatcta aatcaacttt caaacccacg cgtaataaca tcatggcggc
    27721 gcggacacgc atttctcgcc agtcgataat attgacgcca cagatttttt tcgtcagatg
    27781 acgaccaata tataaatttt ccagtacggt taattcatca ataacgctga gttcctgata
    27841 aataatcccg ataccgagtt gtgccgctaa tttatgatcc agcttgttat agttaatgtt
    27901 attaatagta atggtgcctt tggtcggctc atgtattccg gataaaacct tcattagcgt
    27961 ggatttaccc gcgccatttt ctcctagtaa tgcatgtatt tcaccaggat aaaccgttaa
    28021 attaaccgac tttaatgcgt gaaccggacc aaaggacttg ccgatccccg ccatcgatat
    28081 atatggcgtg gccataaata acctctctgc acgaaatatc gtattattgc ctgatggcta
    28141 cgtttatcag gtctacgttc aggttatttt gttggccgga taagacactt cattagcatc
    28201 atcatccggc atcattcagt tttattgagt gaccaggatt gaatcgacca gtttaaattc
    28261 cggtgcttta tccagcggaa taaccttgcc ggatttctca gcgtcaacca tcagcttcag
    28321 acccgttgcg ccaatatctg ccgggttctg ggcaaccgtc gcggtcattt gtccggcttc
    28381 caccattttg cgggcttccg gaattccatc tgtaccgacg accaatattt tgcccgtttt
    28441 tccggcgttt gcgactgcct gagcaacacc cattgccatc gtgtcattcg cgcaatagat
    28501 cgctttaata ttcggattac gttgcaacac gttagtggcg acatccagtg ctttaatgcg
    28561 gtcccagtcg gcaggctggc tggcgacaag cttgatctgg cttgcttttt tgaaggcttc
    28621 ggtggcacca ttacgacgcg cttcaccgga ggcgttaccg gctttaccct caatgattgc
    28681 gacttcacca ccttcagcgc ccaatttgtc aataatgaac gacgcgcctt tcgccccgac
    28741 agcaacgtta tcggtggtga caaaaccttc cacattgccg ccagcttttt tcagattatc
    28801 catgtcgatt ttttcatcga gattaaccag ataaatgcct tttttccatg cgcgggcgac
    28861 aggcatgacc agattcactg aggataatgg agcgaaggcg atgcctttgt aatttttatt
    28921 actgagatct tcaaataact gcaattgaga ttgaaaatcg ccttctgaag gagaggcaaa
    28981 aatatcaacg ctgacaccca gcgtttttgc ttcatcttca atgccttttt tcatatctac
    29041 ccaaaatggg ttggagagcg ttttcaatac gacagcatat tcggcggcag caaaagcgct
    29101 ggttgacaac attaagccca cgagtgtgcc gctgaaatat ttcagatatt tattcataat
    29161 aatgttctca cggtaagggg acaggtaact ccgcgatggc ggaattacct ttttcttatt
    29221 tcagcgcgcc tggtgagaag aaatcaacaa ttgcgccggt tttttgcata ttaatattag
    29281 cttgttcaat gttgagctgg gcgacagaga caaaaaaagc gtccagcaaa gttagttgta
    29341 atattcttgc cgaggcatta cgacctaata acggcgtttc cggggctggt gagcaaataa
    29401 tataatcggc cagtttcgct atcggtgaat ggtagctatg ggttatacaa ataatctttg
    29461 ccccgttctt ttttgccagt tctacggccg cttttacatc actggttcgc ccggaatggg
    29521 tcactaccag cacaacatct ccttcctgta acaacgaagc ggacatcatc atgatgtgag
    29581 cgtccggata ggcctgacag cgtacgccaa tgcgcaagaa cttgtgctgt acatcagcac
    29641 agatagcatt tgatcctccg gcaccgtaca aatcccgctg tctggcctga tagaaaaagc
    29701 gggcggcacg gtggatctca tcaacgttga cgatcgactg accttccata atggtgcgta
    29761 aagtgatgtt aaatacctta ttcaccacat cctgcggcgc ttcatcaaaa gccaactcgg
    29821 aaggcaatac ctgttctgac tgagaaaaat aatcttccag cgcactgcgt aagttacgaa
    29881 agccgctaaa ccccagcagc tttgatacct taactatcat cgcttcagat accgccagag
    29941 cttctgcgac atctttaatt gcgggtgcac aactcaggtt accgggtttg agtaaccact
    30001 ccacgatgcg gctttcattt tctgtcattc cttcctgctt cattcgcagg taaggcgcta
    30061 accctatacc gttcggaagc gctgaatcaa actctgactg gctcatcacg ttctcttgtc
    30121 cttgaattga agacgccact atatcaccac agcaagaaca gaaaagatgc tggcgacttc
    30181 acaaaaaaat aaaatttata aagtttattt tattgtgaac aatggcgagt ggcttaaaat
    30241 tttatttcat ttaattagtt taaaaacatg atgttatttg tttttctggt tttttagatg
    30301 atagatgttg ctggatggtg agtgtcttta acgctattgc gctgtcagat gtaactctgt
    30361 aaaacagatc aggaaggcgt aattcatcga ttttgggtgt ttttaattca ttaacatcac
    30421 aaatgttttt tgattgtgaa gttttgcacg gacggggaag atgaatgaaa aagattgcat
    30481 ttggctgtga tcatgtcggt ttcattttaa aacatgaaat agtggcacat ttagttgagc
    30541 gtggcgttga agtgattgat aaaggaacct ggtcgtcaga gcgtactgat tatccgcatt
    30601 acgccagtca agtcgcactg gctgttgctg gcggagaggt tgatggcggg attttgattt
    30661 gtggtactgg cgtcggtatt tcgatagcgg cgaacaagtt tgccggaatt cgcgcggtcg
    30721 tctgtagcga accttattcc gcgcaacttt cgcggcagca taacgacacc aacgtgctgg
    30781 cttttggttc acgagtggtt ggcctcgaac tggcaaaaat gattgtggat gcgtggctgg
    30841 gcgcacagta cgaaggcggt cgtcatcaac aacgcgtgga ggcgattacg gcaatagagc
    30901 agcggagaaa ttgagattca tccactactt gcatggatga gtaatgacta atgtggatag
    30961 agtttctttt tgaggttggc taatgaaacg ctttccgcta tttcttttat tcaccctgct
    31021 cacgctgtcc accgttccgg cccaggccga tattatcgac gacaccattg gcaatattca
    31081 gcaggcgatt aacgacgctt ataaccctga ccgtgggtga gactatgaag attcgcgcga
    31141 cgacggctgg cagcgtgaag tgagcgacga tcggcgcaga caatatgacg accgccgccg
    31201 ccagtttgaa gatcgccgca ggcagctgga cgatcgccag cgccagcttg atcaggagcg
    31261 tcggcagcta gaagatgaag agcggagaat ggaagatgag tatgggcggt gaggtaggct
    31321 agggtgaggg ggccagcgcg cacattccct ctcatcacgc caccccaatc tccatcccat
    31381 caaacccggc ttcaaacccc ggtggcagcg cattctccat cagccacgca tcaaactggt
    31441 ggctgatatg ggtcagaatc acccgtggcg agcggataac ctgattcagc gcaagcacgg
    31501 tatttaaatc acagtgatta cgcggcgcat cctcgcgcgg cgggtggctg caatcaatca
    31561 ccatcacctg cggatgatta ttgagtaaaa atttctgtgt tttttccggc aggccagcgg
    31621 tgtcagatag ccacgccacc cggctatgtg ccgtttccag cagataaccg aaggtcagtt
    31681 ttgagtggtt aagcggcagc ggcgtgacct gcaacccctg caaatcaaac accacaaacg
    31741 gttccactgt gtggctgaaa tcaagcaggc ccggatgttt aaacagatcg tcgcaaccct
    31801 ggtcatccgg cgggccgtaa accgggatcg gatcgccaac gccccagcgc agcggaaaca
    31861 gcccctggac gtgatccata tgataatgcg tcagcaaaaa ctgctggaac gatccgggcg
    31921 accagcgatc ggcgagatcg tgcagcccgg cgtcgatcag ggtgattgcg tcgttaaact
    31981 tcactacgcc gctgcatggc tggcggcgat actgcggtga gcgccgcgct cggtcgcagg
    32041 ccgcacactc gcagccccat gccggaacgc cctgtgcgcc gccggtgccg gtgagcgtga
    32101 gggtcaggct catgttacag cgccttggtg aagcggaagt ggctctgctc gtagccttca
    32161 cgcaggtaaa aacggtgcgc gtcgtggcgc ttcacgttgg tggaaagttc ggtcatttcg
    32221 gccccggcct ggcgggcttc ttcttctgcc cacgccagta atttactgcc gacgtttaga
    32281 ccgcgcgcct gcggcatcac caccagttcc tgaatttcgc cgatccagtt gacatgatgc
    32341 agatgaaact gcaaatgcag gccgatcatg ccgacaactt cgccatccag cagcgccaga
    32401 tggtagcgca tgtttgggtc gcgcagattg gcgttaaagc ccacgcgaaa cgcgtggtgg
    32461 tcaaactccg cctgttttag ctcacaaatc aacgcgtaaa ccgcgtcggt gtcgtactgc
    32521 gtggccgggc gaagctcaca agcaggcatg gtgtttctcc ttctgatgga tcagcgtcag
    32581 cagcgtgtcg accgactggc gcaggctgcc gtcattgttg agcgtatggc aatcctgtgg
    32641 agtatagcgg gcggcgcgcg ccaggcgggc gttaatttca ctggcgtttt cacggccacg
    32701 gttttccagg cgctggcgga ggatctccgg cgaaacctgt aaacagacgg gcagcagcgc
    32761 cgattgatag cgcgcccgcg cctgcggcag atgggcgcgt gagccgttga ccagcacgtc
    32821 gaatccggcg tgcagccaga ggtcgatctc gacgccgacg ccataataca gaccgttagc
    32881 gtgccagctt aaggccagta ggttctgccc cgcgcgggta aaaaactcct gctcgctcag
    32941 ggcgatatgg ttttcgcttc cggcgctggc gtcgcgcgtg atgtagcgat gcgccaccag
    33001 tagctgagtt tgttcccgca ggcggagttc cgccagcagg ctatctttcc cggagccgga
    33061 cggccccatt aaccaaatca gttttcccat catcagaaca cccttttacc ctgacgccag
    33121 acgtggtcga tatgaatgtg attgcccttg cgatgcgcca gcaccaggtc cgcgcgtttg
    33181 ccctcgccaa tcaccccgcg atcctggaga ttaagcgcct gcgctggatt tttagtcacc
    33241 agcttcaccg cctgcggcag cgtaaagcgg ttgctctggt catcggcgac gcgaaatgcc
    33301 gcatcgagca ggctggcggg gtagtagtcg gaagagagga tatccagcag gccaagctgc
    33361 gccagttcac tggccgccac gttgccggag tgcgagccgc cgcgcacaat attcggcgcg
    33421 cccatcagca cgttcatgcc atgcttgcgc gaggcttccg ccgcttcgaa cgtggtggga
    33481 aattcggcga tcacgctgcc aagctggtga gattcagcaa cgtgggcgtg ggtggcgtca
    33541 tcgtggctgg caagcgcaat ttttcgcgcg cggcacaggg cggcgatgga ttcgcgattc
    33601 ggctgcgacc agcgtgcggc gagcgccagt tgctcttctt cgtactgctg catctgcgca
    33661 tcagtgaggg agtatttgcc ctgataatat tcgcgatact tctcgcggtt ggcgaactgg
    33721 cgctggcccg gcgagtggtc catcagcgac accagcgtca ccggctcgcg ctgcaccagt
    33781 ttttcaaaca gcggcagcgt ggtgtgatgc ggcagttcgc agcgcagatg cagacggtgc
    33841 tcggcgcggt tgacgccgcg tttctgcgtc tcttcgatgg cgttgatcat cttctccaga
    33901 ttctccagcc gatcgccgcc gtcgcgcacg tcgccaattg ccacggcatc cagtacggtg
    33961 gtgatgccgc tcgccaccat cagcgcgtcg tggctgctca tcgccgagtg ggcaggccag
    34021 tcaactttcg ggcgcggggt gaagaattta tccagattat cggtatgcag ctcaatcagc
    34081 cccggcagca gccagccgcc ttcgccgtcc atcgcctccg gcaggcggct ctggctttcg
    34141 gcaaaggcgc ggatttcgcc gttctgcacc tccagcgaac cgcttaccac ctcgttttcc
    34201 agcaccagct taacgttatt gataatcatg aagaggctcc cattgggtgc aggcggtcgg
    34261 cgacgtcatt acgtacagct tcgtcatgga agatgcctac gatggctgcg ccacgggttt
    34321 tggcttcgcg aatcagttcc accaccgcgg cgctgttttt ggcgtccagc gaggcggtag
    34381 gttcgtcaag cagcagaatg gggtagtcga cgataaagcc gcgggcgatg ttgacgcgct
    34441 gctgttcgcc accggaaaat gtcgatggtg ccaggtgcca caggcgttcc ggcacgttca
    34501 ggcgggtgag aagacgcgcg gctttagcgg cgcaggcttc acgcggaacg ccggtatcga
    34561 gcagcggctg catcaccact tccagtgctg agatacgcgg gatgacgcgc agaaactggc
    34621 tcacccagcc gacggtggtt ttgcggattt ccaccacttt gcgcgctggc gcggtgacca
    34681 ggtctaccca ctcgtcaccg tgtttgatct ggatttgacc ttcgtcgggc agatagttgg
    34741 cgtacagcga gcgtagcaga gttgatttgc cgctgccgga atggccgtgg agcaccacgc
    34801 attcgcccgc gttgacggtg agcgaggcgc gattgaggac gggcaggcgc acgccgtttt
    34861 gctggtgcag gatgaaggtt ttactgacgt tttgtacgtt aatcattttt cgcctcgtgg
    34921 ttcgggtgtc ggatgcgacg ctgacgcgtc ttatccgacc tacggggagc gcatttgtag
    34981 gccggataag gcgtttacgc cgcatccggc accggctcaa ttctgcaaaa ccgatgacac
    35041 cagcagctgt gtatacggat gatgcgggtc gtcgagcacg cggtcggtta acccactctc
    35101 caccacttgc ccctgcttca tcaccagcaa acggtccgcc agcaggcggg cgacgcctaa
    35161 atcatgggtg acaatcacca ccgcgaggtt cagctccacc accaggccgc gcagcaggtc
    35221 gagcaggcgg gcctgcaccg acacatccag cccgccggtc ggttcatcca taaacaccag
    35281 cttcggatgc gtcaccaggt tgcgggcaat ctgcaaacgc tgctgcatac cgccggaaaa
    35341 ggtggtcggc aggtcgtcga tccggttggc gggaatctcc acctcttcca gccacttctg
    35401 cgcggtggca cgaatatcgc cgtaatgacg tgccccggtc gccatcgcca tcgctttgcg
    35461 ctcgctcatg ccgaacacca gcccgtagcc gcgcgtgaag tgcggcggct cacccggcgg
    35521 gtcgataaaa ccgttaacca tttcacactc agtcatcagc agttcgccga cgttcaccgc
    35581 aaatcccagc tcttccggca caatcgacac gtcgatatag ccgctgcgga tctcgcctgc
    35641 gaacgggtga ttgcgcccgt aaccgcgttg ggtggagtag gccagcgcca gcaaatagcc
    35701 ttcgtcgccg cgcatcaact gctgcaaacg ggaggagcgt gagcaggggt aaaccggcgg
    35761 cgtgcgggtg atgtcatccg gctgtgcgcc gctatcctct tcaaacttcg ccagcccctg
    35821 acgcgccagc aggctgaaaa cgtgcggcga cggctgctgt tcgctgtcgg cggtggtcag
    35881 cgtcggcgct tcgccgtttg ccagcagggt aaaatcgagc aggcgatggg tgtagtcgta
    35941 ggttgggcca agcagctggc cgccgggaat gtctttataa acggcggaga tacggcgttc
    36001 gagacgcatc ccggtggtgt cgagcggctc gcttaccgcc agcttcgcca acgtggtgcg
    36061 gtaggcgcgc agcaggaaaa tcgcttcaac gttatcgccg ctggcctgtt tcagcgccag
    36121 cgccgccagt tcgcggtcgg caatgccgcc ttcggtcatc acgcgatcta ccgcgaggtt
    36181 aagctgctgt tcaatctggg cgacgctcag ttcgggcaaa tcggtatcgc ctcggcgtcg
    36241 gctctcttgc agggcgtggg cggcgtcgat cgccttctcg ccccctttca cggcaacgta
    36301 catcagcaca cctccacatg agtggttcgc ggaatagcca gcaggcgctc gccacaggtc
    36361 aggatcaggt cgatgccgag cgggaacgga tgcgggcgct cggtgagttc gtgcagaatg
    36421 cactccggca gctgcggagc gatcattcgt tcttcggcaa tacccgcacc agtaaggcgc
    36481 aacatgcgtc cgccgctcag gctggcgacc tgtaaaatca gcgtcgcacc cgcttccggc
    36541 gcaacggcgg tgccggtgga aagggcgttg agctgttcgc tggaaatcgc ctcatccgtc
    36601 accgcgaagg tcgcctgttc cggctggctg accagcggcg cgttggtatg aaaacgcagg
    36661 ctctggttga cgatatcgtt atttaatggg gtagaaagcc acaccggcgt gtcgttatcg
    36721 gccagcgtca gcagcacgct ggtggtggcg atattcagcg gttgccagcc gcgtttgagc
    36781 tgatgcaggg cgacaatcac gcccggctcg ctcatggcct ttaacaggcg acgaaaactg
    36841 tgctgggcat cctgcacggg aagcataaaa gcggtttcca gggtcatgcg ttgtctccgc
    36901 gaaccatcgt aaagaagtcg acccggctgg cgttcacttc ggcctggcgt gcggcaatgc
    36961 gtgccatacg gtcagcgtcc agcggggcaa taagggtttc tgataagttt tgaaagtggc
    37021 gagattgctg catcagcgcg tcaatcagcg cgcagcgttc ggcgtgctgt ttatcacgcc
    37081 cctgcaccca gctgtagccg agcgtgccgt cagtcaggcg cacggcggcg cgggtcagcg
    37141 tggcgtcgcc ggcaaaaaaa cgttcgccgg tgccgcccat ccgcgcctga atctgtacca
    37201 ggccagtttc agcggcgcgg atcacctcat agtcggcggt gatgtttagc gcgttcaggc
    37261 gtgctgccag ttcagccggt tggctgtgcg ccagcacgga catccagtgc tggcgggtcg
    37321 cggtatctgc gtgcattcag tgctccatag tgaattcaat catgtcggcg cgcgtcaggc
    37381 tgacggagta ctccgccggg ctgctttcac cgtcacggtg gttaagggtg cgcacgcaca
    37441 gcagcggcga catattcggg atttcaagac gctggcactc tttggcctgg gcgcggcggg
    37501 cgctgatccg cgtctggctg cggcgcagcg caattccggt ttgctcgcgc agaaaatcgt
    37561 gcagcgagcc gctgtcgaag cgttgcagcg tcggccagag ggtgaggtcc gcgaagtagt
    37621 ggtcgattaa acagagcgcg acgccgttga cacgacgcag ggtgcgcagg tggatgacgt
    37681 tctccccctc ggtaatcccc agtgcgtcag cgacgtggcc ggacgcgggg cgcaataccg
    37741 aaagcagttt ttcgctggtg ggatggctgc cctgatccag cagattctgg ctaaaacgcg
    37801 cctgggcgtt gagcgggtaa tcgaacgggc gcatcagcac cagcacgccg acgccctgac
    37861 ggcgctgtac ccagcctttt tccaccagtt ggtcgatggc gcggcgcagg gtgtggcgat
    37921 tcacctcaaa gcgcgctgcc agttgctgct cggcgggaag atagtcgccg cagcggtagt
    37981 gttgacgaag ctcctgctca agttttgcgg ctatctcttg atagcgtgtt gggtagctgg
    38041 tcggatgtgt agacaagtgc atagatatca atgcctcgct tatcagataa agtgcttacg
    38101 caaccgttga gagaggaaat ccagcaggct gaccgtgacg atgataagca ccatcagggc
    38161 gcaggtttgt tggaactgga aaccgcgaat cgcttcccac agggtgacgc cgatcccgcc
    38221 tgcgccgacc atgccgacga cggtcgccga gcggacgttg gattcgaagc gatagaggga
    38281 gtaggagatc agcagtggca tcacctgtgg cagcacgccg tagaggatct cttcgagctt
    38341 gttggcaccg gtggcgcgaa tgccttccac cgggccgggt tcaatcgctt ccaccgcttc
    38401 ggaaagcagc ttggagagca cgccggtggt gtggataaac agcgccagca cgccagcacg
    38461 ccagcgaacg ggccgaggcc gacggcgacc acgaacagca tggcgaagac catttcgtta
    38521 atggcgcggc aggcgtccat caggcggcga acgggctggt aaacccacca cggcaccagg
    38581 ttttcggcgc tcatcaggcc aaaggggatg gagagaacca ccgccagcgc ggtgccccag
    38641 acggcgattt gcagcgtgac ggccatttcg gtgaggtaat cctgccactg gctgaaatcg
    38701 ggcgggaaga agtcggcggc gaacgtcgcc atgttgccgc cgtctttgat cagcgtaagc
    38761 ggggccattt ccgcgccctg ccacgagacg accaacacgg cgagtacaac ggcccagctc
    38821 agaagcgaga accagctgcg cttgggtggg gcgatggtga tggtttgcat gtttggctcc
    38881 ggtctgtagg ccggataagg tgcttgcacc gcatccggca tcaacgcctg cacattgtcg
    38941 gatgcgacgc cggggcgtct tatccgacct acgatttact gcaccgcttt actcaccgaa
    39001 ctcatcgcgc ttagcgcgtt gttcaggcgg tccaggtcat ccagctgcgc ctgaatctcg
    39061 gtggtttttg ccagcttgtc ctgctcattc agtcctttat tgcttttcac gccctgcatc
    39121 tctttaaaca gcgcgagctg gcgaatcggc accagttgca ggtcgctgga agcgcggaat
    39181 ggcgcccagc ccaggcgttc cagcaccgct ttttcttccg gcgttttgcc gtagttcata
    39241 aagaagtcgt agatcttgtc tttggtggtt tcggaaagat tcttgcgcca gacgatcgga
    39301 tcgcctggga tcagcggcga cttccagatc accttcagtt ctttcagctt ctctggcgcg
    39361 gaggttttca gcttgtcgag gttttcggtg ttgttggtgg caacatccac ctgcttgttg
    39421 gcgacggcca gcgcgttggt ttcatgcccg gcgttgacgg tgcgcttgaa gtcgctggcg
    39481 gagatattgt ttttggcgaa gacgtagtag ccggggacga ggaagccaga ggtggagtta
    39541 ggatcgccat tgccaaaggt gagatctttc cgcttcgcca gcagatcgtt caggttgttg
    39601 atcggactgt ctttgttgac gatcaacacg ctccagtaac ccggcgatcc atccgccgcg
    39661 accgtctggg cgaagacctg gccattggcg cgatccaccg cttccatcgc cgacagattg
    39721 ccgtaccagg cgatatccac tttattgaag cgcatcccct ggataatgcc cgcgtagtcc
    39781 ggggcaaaga aggcgttgac cttcacgccc agcttcttct ccatatcctg caagaacggc
    39841 gtccattgcg gtttcaggtt ttgctgtgat tccgttgaaa taatgccgaa attcaacgcc
    39901 ttttcctgct cttcggcgtg tgccgggctt aacagggtgc tgaggctgaa catgctggtg
    39961 aaggccagcg aggcaattat cttagcgttc attcgttttc ctcaatgatg gggatgtcag
    40021 gcagctttcg cgttctcttc gatgcgatta atgctgcggt agagatggtc aaaacgttcg
    40081 ttatcaaact gttggctgct gccgtcgtag aaaacgtgcc cctggcgcag ggcgacgatg
    40141 cgttcgcagt agcgcagggc gtaatccacc tgatgcagcg tgacgaccac ggtgatgccg
    40201 tcgttctgat tgatgtcgcg cagggtgtcc atcacgatgc gcgctgattc tgggtccagc
    40261 gaggcgatgg gttcatcggc cagaatcact ttcgcctgct gcatcagcgc acgggcaatc
    40321 gccacacgtt gctgctggcc gccggagagg gtggaaacgc gctgatgggc aaaatgcacc
    40381 atgccaacgc gggtcagcgc ctgtaacgcg cgctgtttct gctcgccggt gaaccagcta
    40441 aaacaggtgc gccagaacgg cgtgctgccg agcgcgccaa tcagcacgtt ctccagtacg
    40501 ctcaggcggt tcaccaggtt gaattgttgg aatatgtagc cggtatgggc gcggcttttg
    40561 cggatatcgc gggccaggcg gccttcgcgc tggactgtgc ggcccagcag ctcgatatgg
    40621 ctaccgacag atttatcgcc ggtaatcaaa ccgcttaagt gacgtaaaag ggtggatttt
    40681 ccggaacccg acggcccaag cagagccacc atttcaccgt gatgaatgtt cagatcaacc
    40741 gcatgcagcg cctgatgctg attgaaggtt ttggcgagct tctcgacacg gataatcgtt
    40801 tgcatgatgc agcctcccta aaaaagtggc tccatcgtgg cggattattg tgacgatttg
    40861 gttaattaaa agtgactaac agatgaagag ttaacgggaa ttcgatgaca gtgcgggagg
    40921 gcgggcctcc cggcgggtta ttgcgttggt tgttgtttga cgacattaat catccacggt
    40981 acgccaaatt tatcggtgac tttgccaaag ccatgcgccc agaaagtttc ctgccaggcc
    41041 atttcgattt ttccgttagc ggcaagattg tcaaaccagc gttttccttc ttcgacctgt
    41101 tgcgaatcga gcaccagcgt aaagccggag tagctggctt ttcctgacgg catggcatcg
    41161 ctcatcatga tgtcgcttcc ggcaatgcgc acattggcgt gggcgatggc ggtatcggga
    41221 aattgcattc cggaagggca gttctcggcg ctgtcctgcg ctgattttgg catttcgccg
    41281 aagctgattt tatagagcag ttccgcgccc aacgtacgtt gataataggc aatcgcgtcg
    41341 gaacagttac cggcaaaaga gaggtaggga cttaacggca tgatggtgac ctcagttaag
    41401 agaagccagt taagtgtagt tcgaaattta tacagatgag aggcggcctg ataagacgcg
    41461 cgagcgtcgc atcaggcagt cggcactgtt gccggatgcg gcgtaaacgc cttatccggc
    41521 ctacggaggg tgcgggaatt tgtaggcctg ataagacgcg caagcgtcgc atcaggcagt
    41581 tggcactgtt gccggatgcg gcgtaaacgc cttatccggc ctacggaggg tgcgggaatt
    41641 tgtaggcctg ataagacgcg caagcgtcgc atcaggcagt cggcaccgtt gccggatgcg
    41701 gcgtaaacgc cttatccggc ctacggaggg tgcgggaatt tgtaggcctg ataagacgcg
    41761 caagcgtcgc atcaggcatc tggcaccgtt gccggatgcg gcgcgagcgc cttatccggc
    41821 ttaccgatca caatacaatc agttcttttt cacaaactca gatttcagtt tcatcggacc
    41881 aaaaccgtcg attttgcaat cgatgttatg gtcgccttca accaggcgga tgtttttcac
    41941 tttggtgcca attttcagca tcgaagagct acctttcacc ttcagatctt taatgatggt
    42001 aacgctgtcg ccgtcagcca gcagattgcc gttagcatct ttaacgatca gctcgtcgct
    42061 ttcctgtgca ggttctgcgt cgttccattc gtaggcacat tccgggcaga tgtacatgcc
    42121 gttatcttcg taggtgtatt cggagttgca ttttgggcag tgtggtaatg acatgtggat
    42181 ttcctcaaaa gtcagcaggg caaaagcgcc aaaaaacggc agattgccga aaaaggccgc
    42241 aattatacac aaaatccctg agtttgtcgg gggtactgct gattttagac gacgatcgtt
    42301 ttcatttgaa aggaaacgca aaaagaaaat aatct
//
LOCUS       DAZFZC010000036        39010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFZC010000036 DAZFZC010000000
VERSION     DAZFZC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.036"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(189..332)
                     /gene="ypaB"
                     /locus_tag="ACHKXP_002195"
     CDS             complement(189..332)
                     /gene="ypaB"
                     /locus_tag="ACHKXP_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO7647196.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            460..2745
                     /gene="nrdA"
                     /locus_tag="ACHKXP_002196"
     CDS             460..2745
                     /gene="nrdA"
                     /locus_tag="ACHKXP_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7647197.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2979..4109
                     /gene="nrdB"
                     /locus_tag="ACHKXP_002197"
     CDS             2979..4109
                     /gene="nrdB"
                     /locus_tag="ACHKXP_002197"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7647198.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4109..4363
                     /gene="yfaE"
                     /locus_tag="ACHKXP_002198"
     CDS             4109..4363
                     /gene="yfaE"
                     /locus_tag="ACHKXP_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO7647199.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4417..5067)
                     /gene="inaA"
                     /locus_tag="ACHKXP_002199"
     CDS             complement(4417..5067)
                     /gene="inaA"
                     /locus_tag="ACHKXP_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO7647200.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5147..5251)
                     /locus_tag="ACHKXP_002200"
                     /pseudo
     CDS             complement(5147..5251)
                     /locus_tag="ACHKXP_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5246..5488
                     /locus_tag="ACHKXP_002201"
                     /pseudo
     CDS             5246..5488
                     /locus_tag="ACHKXP_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5530..6606)
                     /gene="glpQ"
                     /locus_tag="ACHKXP_002202"
     CDS             complement(5530..6606)
                     /gene="glpQ"
                     /locus_tag="ACHKXP_002202"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7647201.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDNLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6611..7969)
                     /gene="glpT"
                     /locus_tag="ACHKXP_002203"
     CDS             complement(6611..7969)
                     /gene="glpT"
                     /locus_tag="ACHKXP_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO7647202.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8242..9870
                     /gene="glpA"
                     /locus_tag="ACHKXP_002204"
     CDS             8242..9870
                     /gene="glpA"
                     /locus_tag="ACHKXP_002204"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO7647203.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9860..11119
                     /gene="glpB"
                     /locus_tag="ACHKXP_002205"
     CDS             9860..11119
                     /gene="glpB"
                     /locus_tag="ACHKXP_002205"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO7647204.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVHQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11116..12306
                     /gene="glpC"
                     /locus_tag="ACHKXP_002206"
     CDS             11116..12306
                     /gene="glpC"
                     /locus_tag="ACHKXP_002206"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO7647205.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12499..13398
                     /gene="rpnE"
                     /locus_tag="ACHKXP_002207"
     CDS             12499..13398
                     /gene="rpnE"
                     /locus_tag="ACHKXP_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO7647206.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLATTQLTDADIPNCH"
     gene            13399..13596
                     /locus_tag="ACHKXP_002208"
                     /pseudo
     CDS             13399..13596
                     /locus_tag="ACHKXP_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13637..14440)
                     /gene="yfaU"
                     /locus_tag="ACHKXP_002209"
     CDS             complement(13637..14440)
                     /gene="yfaU"
                     /locus_tag="ACHKXP_002209"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO7647207.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     EQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14458..15747)
                     /gene="yfaV"
                     /locus_tag="ACHKXP_002210"
     CDS             complement(14458..15747)
                     /gene="yfaV"
                     /locus_tag="ACHKXP_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7647208.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLFGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(15804..17009)
                     /gene="rhmD"
                     /locus_tag="ACHKXP_002211"
     CDS             complement(15804..17009)
                     /gene="rhmD"
                     /locus_tag="ACHKXP_002211"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO7647209.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLSATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYESYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(17024..17806)
                     /gene="yfaX"
                     /locus_tag="ACHKXP_002212"
     CDS             complement(17024..17806)
                     /gene="yfaX"
                     /locus_tag="ACHKXP_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO7647210.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNVNNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(18026..19228)
                     /gene="yfaY"
                     /locus_tag="ACHKXP_002213"
     CDS             complement(18026..19228)
                     /gene="yfaY"
                     /locus_tag="ACHKXP_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO7647211.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(19328..19870)
                     /gene="yfaZ"
                     /locus_tag="ACHKXP_002214"
     CDS             complement(19328..19870)
                     /gene="yfaZ"
                     /locus_tag="ACHKXP_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family protein"
                     /protein_id="HFO7647212.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTETT
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            20149..20574
                     /gene="nudI"
                     /locus_tag="ACHKXP_002215"
     CDS             20149..20574
                     /gene="nudI"
                     /locus_tag="ACHKXP_002215"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO7647213.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWAISGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(20613..21215)
                     /gene="ais"
                     /locus_tag="ACHKXP_002216"
     CDS             complement(20613..21215)
                     /gene="ais"
                     /locus_tag="ACHKXP_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO7647214.1"
                     /translation="MLAFCRSSLKSKKYIIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            21523..22662
                     /gene="arnB"
                     /locus_tag="ACHKXP_002217"
     CDS             21523..22662
                     /gene="arnB"
                     /locus_tag="ACHKXP_002217"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO7647215.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADHVITALQQLAGQ"
     gene            22666..23634
                     /gene="arnC"
                     /locus_tag="ACHKXP_002218"
     CDS             22666..23634
                     /gene="arnC"
                     /locus_tag="ACHKXP_002218"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO7647216.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            23634..25616
                     /gene="arnA"
                     /locus_tag="ACHKXP_002219"
     CDS             23634..25616
                     /gene="arnA"
                     /locus_tag="ACHKXP_002219"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO7647217.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLIYDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            25613..26503
                     /gene="arnD"
                     /locus_tag="ACHKXP_002220"
     CDS             25613..26503
                     /gene="arnD"
                     /locus_tag="ACHKXP_002220"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO7647218.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            26503..28155
                     /gene="arnT"
                     /locus_tag="ACHKXP_002221"
     CDS             26503..28155
                     /gene="arnT"
                     /locus_tag="ACHKXP_002221"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO7647219.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIEPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            28152..28487
                     /gene="arnE"
                     /locus_tag="ACHKXP_002222"
     CDS             28152..28487
                     /gene="arnE"
                     /locus_tag="ACHKXP_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO7647220.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            28487..28873
                     /gene="arnF"
                     /locus_tag="ACHKXP_002223"
     CDS             28487..28873
                     /gene="arnF"
                     /locus_tag="ACHKXP_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO7647221.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(28867..29133)
                     /gene="pmrD"
                     /locus_tag="ACHKXP_002224"
     CDS             complement(28867..29133)
                     /gene="pmrD"
                     /locus_tag="ACHKXP_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO7647222.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(29243..30598)
                     /gene="menE"
                     /locus_tag="ACHKXP_002225"
     CDS             complement(29243..30598)
                     /gene="menE"
                     /locus_tag="ACHKXP_002225"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO7647223.1"
                     /translation="MIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(30595..31557)
                     /gene="menC"
                     /locus_tag="ACHKXP_002226"
     CDS             complement(30595..31557)
                     /gene="menC"
                     /locus_tag="ACHKXP_002226"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO7647224.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(31557..32414)
                     /gene="menB"
                     /locus_tag="ACHKXP_002227"
     CDS             complement(31557..32414)
                     /gene="menB"
                     /locus_tag="ACHKXP_002227"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO7647225.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(32429..33187)
                     /gene="menH"
                     /locus_tag="ACHKXP_002228"
     CDS             complement(32429..33187)
                     /gene="menH"
                     /locus_tag="ACHKXP_002228"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO7647226.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWVQRFLTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(33184..34854)
                     /gene="menD"
                     /locus_tag="ACHKXP_002229"
     CDS             complement(33184..34854)
                     /gene="menD"
                     /locus_tag="ACHKXP_002229"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO7647227.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLCQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(34943..36238)
                     /gene="menF"
                     /locus_tag="ACHKXP_002230"
     CDS             complement(34943..36238)
                     /gene="menF"
                     /locus_tag="ACHKXP_002230"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO7647228.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(36317..36622)
                     /gene="elaB"
                     /locus_tag="ACHKXP_002231"
     CDS             complement(36317..36622)
                     /gene="elaB"
                     /locus_tag="ACHKXP_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO7647229.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(36677..37138)
                     /gene="elaA"
                     /locus_tag="ACHKXP_002232"
     CDS             complement(36677..37138)
                     /gene="elaA"
                     /locus_tag="ACHKXP_002232"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7647230.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            37203..38120
                     /gene="rbn"
                     /locus_tag="ACHKXP_002233"
     CDS             37203..38120
                     /gene="rbn"
                     /locus_tag="ACHKXP_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO7647231.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            38308..>39010
                     /locus_tag="ACHKXP_002234"
     CDS             38308..>39010
                     /locus_tag="ACHKXP_002234"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO7647232.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SPLKPTAPDNSDDLLFKKLSSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGFYLSNSNSTYLEIALNDNIKSDP
                     SWPKEVQLFPINTGGH"
BASE COUNT         9594 a        10465 c         9622 g         9329 t
ORIGIN      
        1 ttagcattta acaataaccg aatagaaaac aaccatttcg ccatcaacaa tctcttacat
       61 tcgcttatat attgaccaca actgatacat cagattatgt gatgactcgt gcttagatca
      121 atttttgcaa tcattagcaa aaagattaat aaaccatcta tatcaattta tctaacctat
      181 tatgccgttc aagaaatcgc cgaacagtta tttttaacaa atttttctct tcccattgac
      241 tttcccggac accttgtctg acctaaggtg cgcgaaagcc actttttcct tcctgagtta
      301 tccacaaagt tatgcacttg caagagggtc attttcacac tatcttgcag tgaatcccaa
      361 acataccccc tatatatagt gttctaagca gcttcccgta ctacaggtag tctgcatgaa
      421 actattgcgg aaagaattcc aaaaacaggt acgacataca tgaatcagaa tctgctggtg
      481 acaaagcgcg acggtagcac agagcgcatc aatctcgaca aaatccatcg cgttctggat
      541 tgggcggcag aaggactgca taacgtttcg atttcccagg tcgagctgcg ctcccacatt
      601 cagttttatg acggtatcaa gacctctgac atccacgaaa ccattatcaa ggctgccgca
      661 gacctgatct cccgtgatgc gccggattat cagtatctcg ccgcgcgcct ggcgatcttc
      721 cacctgcgta aaaaagccta cggccagttt gagccgcctg cgctgtacga ccacgtggtg
      781 aaaatggtcg agatgggcaa atacgataat catctgctgg aagactacac ggaagaagag
      841 ttcaagcaga tggacacctt tatcgatcac gaccgtgata tgaccttctc ttatgctgcc
      901 gttaagcagc tggaaggcaa atatctggta cagaaccgcg tgaccggcga aatctatgag
      961 agcgcccagt tcctttatat tctggttgcc gcgtgcttgt tctcgaacta cccgcgtgaa
     1021 acgcgcctgc aatatgtgaa gcgtttttac gacgcggttt ccacatttaa aatttcgctg
     1081 ccgacgccaa tcatgtccgg cgtgcgtacc ccgactcgtc agttcagctc ctgcgtactg
     1141 atcgagtgcg gtgacagcct ggattccatc aacgccacct ccagcgcgat tgttaaatac
     1201 gtttcccagc gtgccgggat cggcatcaac gccgggcgta ttcgtgcgct gggtagcccg
     1261 attcgcggtg gtgaagcgtt ccataccggc tgcattccgt tctacaaaca tttccagaca
     1321 gcggtgaaat cctgctctca gggcggtgtg cgcggcggtg cggcaacgct attctacccg
     1381 atgtggcatc tggaagtgga aagcctgctg gtgttgaaaa acaaccgtgg tgtggaaggc
     1441 aaccgcgtgc gtcatatgga ctacggggta caaatcaaca aactgatgta tacccgtctg
     1501 ctgaaaggtg aagatatcac cctgttcagc ccgtccgacg taccggggct gtacgacgcg
     1561 ttcttcgccg atcaggaaga gtttgaacgt ctgtatacca aatatgagaa agacgacagc
     1621 atccgcaagc agcgtgtgaa agccgttgag ctgttctcgc tgatgatgca ggaacgtgcg
     1681 tctaccggtc gtatctatat tcagaacgtt gaccactgca atacccatag cccgtttgat
     1741 ccggccatcg cgccagtgcg tcagtctaac ctgtgcctgg agatagccct gccgaccaaa
     1801 ccgctgaacg acgtcaacga cgagaacggt gaaatcgcgc tgtgtacgct gtctgctttc
     1861 aacctgggcg caattaataa cctggatgaa ctggaagagc tggcaattct ggcggttcgt
     1921 gcacttgacg cgctgctgga ttatcaggat tacccgatcc cggccgccaa acgtggagcg
     1981 atgggtcgtc gtacgctggg tattggtgtg atcaacttcg cttactacct ggcgaagcac
     2041 ggtaaacgct actccgacgg cagcgccaac aacctgacgc ataaaacctt cgaagccatt
     2101 cagtattacc tgctgaaagc ctctaatgag ctggcgaaag agcaaggcgc gtgcccgtgg
     2161 tttaacgaaa ccacttacgc gaaagggatc ctgccgatcg atacctataa gaaagatctg
     2221 gataccatcg ctaatgagcc gctgcattac gactgggaag ctctgcgtga gtcaatcaaa
     2281 acgcacggtc tgcgtaactc cacgctttct gctctgatgc cgtccgagac ttcttcgcag
     2341 atctctaacg ccactaacgg tattgaaccg ccgcgcggtt acgtcagcat caaagcgtcg
     2401 aaagacggta ttttgcgcca ggtggtgccg gactacgagc acctgcacga cgcctatgaa
     2461 ctgctgtggg aaatgccggg taacgatggt tatctgcaac tggtgggtat catgcagaaa
     2521 tttatcgatc agtcgatctc tgccaacacc aactacgatc cgtcacgctt cccgtcagga
     2581 aaagtgccga tgcagcagtt gctgaaagac ctgctcaccg cctacaaatt cggggtcaaa
     2641 acactgtatt atcagaacac ccgtgacggc gctgaagacg cacaagacga tctggtgccg
     2701 tcaatccagg acgatggctg cgaaagcggc gcatgtaaga tctgatattg agatgccgga
     2761 tgcggcgtaa acgccttatc cggcctacgg ctcggtttgt aggcctgata agacgcgcca
     2821 gcgtcgcatc aggctccggg tgccggatgc agcgtgaacg ccttatccgg cctacggctc
     2881 ggatttgtag gcctgataag acgcgccagc gtcgcatcag gcacaggatg cggcgtaaaa
     2941 tgccttatcc ggcattaaac tcccaacagg acacactcat ggcatatacc accttttcac
     3001 agacgaaaaa tgatcagctc aaagaaccga tgttctttgg tcagccggtc aacgtggctc
     3061 gctacgatca gcaaaaatat gacatcttcg aaaagctgat cgaaaagcag ctctctttct
     3121 tctggcgtcc ggaagaagtt gacgtctccc gcgaccgtat agattaccag gcgctgccgg
     3181 agcacgaaaa acacatcttt atcagcaacc tgaaatatca gacgctgctg gattccattc
     3241 agggtcgtag cccgaacgtg gcgctattgc cgcttatttc tattccggaa ctggaaacct
     3301 gggtcgaaac ctgggcgttc tcagaaacga ttcattcccg ttcctatact catatcattc
     3361 gtaatatcgt taacgatccg tctgttgtgt ttgacgatat cgtcaccaac gagcagatcc
     3421 agaaacgtgc ggaagggatc tccagctatt acgatgagct gatcgaaatg accagctact
     3481 ggcatctgct gggcgaaggt acccacaccg ttaacggtaa aactgtgacc gttagcctgc
     3541 gcgagctgaa gaaaaaactg tatctctgcc tgatgagcgt taacgcgctg gaagcgattc
     3601 gtttctacgt cagctttgct tgttccttcg catttgcaga acgcgaattg atggaaggca
     3661 acgccaaaat tattcgcctg attgcccgcg acgaagccct gcacctgacc ggcacccagc
     3721 atatgctgaa tctgctgcgc agcggcgcgg acgatcctga gatggcggaa attgccgaag
     3781 agtgtaagca ggagtgctat gacctgtttg ttcaggcagc tcaacaggag aaagactggg
     3841 cggattatct gttccgcgac ggttcgatga ttggtctgaa taaagacatt ctctgccagt
     3901 acgttgaata catcaccaat atccgtatgc aggcagtcgg tttggatctg ccgttccaga
     3961 cgcgctccaa cccgatcccg tggatcaaca cttggctggt gtctgataac gtgcaggttg
     4021 ctccgcagga agtggaagtc agttcttatc tggtcgggca gattgactcg gaagtggaca
     4081 ccgacgattt gagtaacttc cagctctgat ggcccgcgtt accctgcgca tcactggcac
     4141 acaactgctg tgccaggatg aacacccttc ccttctggcg gcgctggaat cccacaatgt
     4201 ggcggttgag taccagtgtc gcgaaggtta ctgcggctcc tgtcgcacac gcctggttgc
     4261 aggtcaagtt gactggattg ccgaaccgtt agcctttatt cagccggggg aaattttgcc
     4321 ctgttgttgc cgggcaaaag gcgatattga aatcgagatg tgaattggtt gtagtgccag
     4381 atacaacgct tatgcgtctt atctggccta caacgattac atggcgtagt aatacgcttt
     4441 cacctgctcc cagtccgctt tggggattgg ctccagatat ttttccagct ggcggaagtc
     4501 atgattaatc gctttatcgc gacgcaagcg gcgacgactt ttctccaggt caagaaaacc
     4561 agcttctgca ttaccttctg ttttcacata gatatggcga acataacaac agccatgctg
     4621 acgattaatg ctatgcattt tcttaaacgc cagcgccact gctttcaaca tggcttgccg
     4681 tacttcgtca gaataaggcg atactgcatg ctgggcatac cagtcagcaa tgctgatgaa
     4741 ccccgccata tcttcagtca ccagcaacgc tcgccattca ccctcaattt tcaccgcttc
     4801 accaaaaacg atcttcggca cgatgacacc ggcccgttcc agttctttaa ttaccgcaac
     4861 ctcacggaca atcgttggtc ggccgaacgg ataacgtacg gaatgaaaca gatgatgcgt
     4921 catgcgcttt acatacagct ttttgccgtt gcgctcgacg cattgcaccc cgctcatacc
     4981 attacggcga tagttaggct cttcaaccca gtcgccctct gttgcccacc agtgattaaa
     5041 ttcgtcgtac tttgctgaaa ctgccatacc ctatcgcctg tcatttttat taacgacaat
     5101 gactataggt ggttacctga ggaaaatctt aatgaaacgt gtcgtattaa tgaatttctt
     5161 ttgccactac tagcttgaca ccgcttttac ccttcatttg cagcgcatag attccaccag
     5221 ccactaccac taatagtgcg cccagcagat aaaatgtgaa tttaatcagg cggatacagc
     5281 gatgaatttt attcgacagg gattaggcat cgccttacaa ccagagttaa cgctgaaaag
     5341 cattgcaggt gaattgtgtt ccgttcctct cgaaccaact ttctatcgac agatttcgtt
     5401 gctggctaaa gaaaagccgg tagaaggcag tccactgttt ttactacaaa tgtgcatgga
     5461 acaattagtg gcgattggaa aaatttgata tgagacagga tggcgcatga acgccatcct
     5521 gcagtagcat tactctttat taaggaattt tactgcctta tcagggaaat cagtaaacag
     5581 cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat cagtagtgta
     5641 ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat tctgctgagc
     5701 atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtttcct caatcaacat
     5761 atggtaatcc ggcccgatac cgtcggcata ttccgccacc tgtttcatag cacccggctt
     5821 aaacatccag tcgtagttgt aattaaccca gcttccatcc ggctgtttct gctgcgtttc
     5881 attccagtcg gtataggcaa tcagctgtac cagattgaga tccatgccca ttttgggttc
     5941 cagctcattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat aaactttatc
     6001 gtctttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg caatatcctt
     6061 cccttcctga tgatggaacc acggcgcttt gatttctgga taaataccga tatttttccc
     6121 ggtagagtga tttaacccct gaacaaattc aatctcttct tcaaaggtgt gcacccggaa
     6181 gtcggactta cccattggga aacgccccgg ataagtctgc acttttttac cgttttcaat
     6241 atcgaaacct tcggtaaatt tcaacgactt aatttcatcc agcgtgaaat ctatcgcgta
     6301 gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag taacacgatc
     6361 gaggtaatgg tcatgcagaa caaccagatt gtcgtctttg gtcatcacca aatcctgttc
     6421 cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat gctccggcaa
     6481 atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg ccgccattgc
     6541 actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt tcagcgtcaa
     6601 tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga cgtttttcgc
     6661 caatcatcac aacaatcaac aagataaccg ccagaatgct gccgccaatc attaccataa
     6721 agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc gccgccaccg
     6781 aaccgcccag gtaaccaaac agcccggtaa agcccgctgc cgtacctgcc gcttttttcg
     6841 gtgccagttc cagcgcatgc agaccgatca gcatcacagg accgtagatc aggaagccga
     6901 taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc cagtaaacga
     6961 tagtcgcgat ggtcaccagt gtcataaaga aaacgccggt tgccccacgg ttgccacgga
     7021 agactttatc cgacatccag ccgcacagca gagtgcccgg aatacctgca tattcataaa
     7081 ggaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc agataagtcg
     7141 gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggcg atggcgatat
     7201 accacagcag tttgttcggc agcacgtact gcatgaagat ttgcttcgcg gtcagctcct
     7261 gttccgcttt ttcgttatag tcgtccggat aatcattttt gtactcttcg atcggcggca
     7321 agccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaataatgcc accagaatgg
     7381 cgcagaaagc aggcatatag agcgccgcat gccagtcatt gaaccaggcc atccccagca
     7441 ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac actgacacaa
     7501 tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac ggcggccacc
     7561 ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca atgctcgacg
     7621 tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc aaacctgcgg
     7681 gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat ttcgaaaatc
     7741 cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag aatccctgct
     7801 caaccagata aggcatagca agcgcaaagt tcttacgaac caaatagtaa gccgcatagc
     7861 caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga tcgatctccg
     7921 cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt gatagcctcc
     7981 gtggcccgtg gtcttattta tgattaacag cctgattcag tgagagaacc tgccgtttct
     8041 tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattgcc gcacacatta
     8101 ttaaataaga tttacaaaat gttcaaaatg acgcatgaaa tcacgtttca ctttcgaatt
     8161 atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa atggtaaaaa
     8221 acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga cgtgattatc
     8281 attggcggcg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg cgggctgcgc
     8341 gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa ccacggcctg
     8401 ctgcacagcg gtgcgcgcta tgcggtaacc gatgcggaat cggcccgcga atgcattagt
     8461 gaaaaccaga tcctgaaacg cattgcacgt cactgcgttg aaccaaccaa cggcctgttt
     8521 atcaccctgc cggaagatga cctctccttc caggccactt ttattcgcgc ctgcgaagaa
     8581 gcagggatca gcgcagaagc tatagacccg cagcaagcgc gcattatcga acctgccgtt
     8641 aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc atttcgtctg
     8701 accgcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac cgctcatgaa
     8761 gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg taaccatctc
     8821 accggcgaaa ctcaggccct tcatgcacct gtcgtggtta atgccgctgg gatctggggg
     8881 caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa aggatcgctg
     8941 ctgatcatgg atcaccgcat taaccagcat gtgatcaacc gctgccgtaa accttccgac
     9001 gccgatattc tggtgcctgg cgataccatt tcgctgattg gtaccacctc tttacgtatt
     9061 gattacaacg agattgacga taatcgagtg acggcagaag aggttgatat tctgctgcgt
     9121 gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc ctattctggc
     9181 gtgcgcccgc tggttgccag cgatgacgac ccgagcggac gtaacgtcag ccgtggcatc
     9241 gtgctgctcg accatgctga acgcgatggt ctggacggat ttatcaccat caccggtggc
     9301 aaactgatga cctatcggct gatggctgaa tgggctaccg acgcggtatg ccgcaaactg
     9361 ggcaacacgc gcccctgtac gactgccgat ctggcactgc ctggttcaca agaacccgct
     9421 gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc tgcggtttat
     9481 cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctgcaccg tagcctggta
     9541 tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa tttaaacgtt
     9601 aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg ccagggcgaa
     9661 ctctgcgcct gccgcgctgc cggactgctg caacgtttta acgtcacgac gtccgcgcaa
     9721 tctatcgagc aactttccac cttccttaac gaacgctgga aaggcgtgca acccatcgcc
     9781 tggggagatg cactgcgcga aagcgaattt acccgctggg tttatcaggg attgtgtggt
     9841 ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggcg gcctcgccgg
     9901 attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg tcacgcgtgg
     9961 tcaaagcgcg ttgcatttct catccggttc gctggatttg ctgagccatc tgccagatgg
    10021 tcaacccgtg gcagacattc acagtggact ggaatctttg cgtcagcagg cacctgcaca
    10081 tccttactcc cttctcgggc cacaacgcgt gctcgatctc gcttgccagg cgcaggcatt
    10141 aatcgctgaa agcggtgcgc aattgcaggg cagcgtagaa ctggctcacc agcggattac
    10201 gccgctcggc actctgcgct ctacctggct aagttcgcca gaagtccccg tctggccgtt
    10261 gcccgcgaag aaaatatgtg tggtcgggat aagcggcctg atggattttc aggcgcatct
    10321 tgcggcggct tcgttgcgcg aactggatct ttcggttgaa accgcagaaa tagagctgcc
    10381 ggaactggat gtcctgcgca ataacgccac cgaatttcgc gctgtgaata tcgcccgttt
    10441 ccttgataat gaagaaaact ggccgctgtt acttgatgcg cttattcctg tcgccaatac
    10501 ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac tgtggcgttg
    10561 gttgaatgaa aaactacctt gttcactgat gcttttgcca acgctgccgc cttccgtgct
    10621 gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg caccagggtg gcgtgtggat
    10681 gccgggcgat gaagtgaaaa aagtgacctg taaaaatggc gtagtgaatg aaatctggac
    10741 ccgcaatcac gccgatattc cgctacgtcc acgtttcgcg gttctcgcca gcggcagttt
    10801 ctttagtggc ggactggtag cggaacgtaa cggcattcgc gagccgattc tcggccttga
    10861 tgtgctacaa accgccacgc ggggtgaatg gtataagggc gatttttttg cgccgcaacc
    10921 gtggcagcag ttcggtgtaa ccactgatga gacgctacgc ccgtcgcagg cagggcaaac
    10981 cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca tcgcccaggg
    11041 atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgcc gctcaacaga ttgcccaacg
    11101 cgcaggaggc caacaatgaa cgacaccagc ttcgaaaact gcattaagtg caccgtctgc
    11161 accaccgcct gcccggtgag ccgggtgaat cccggttatc cagggccaaa acaagccggg
    11221 ccggatggcg agcgtctgcg tttgaaagat ggcgcactgt atgacgaggc gctgaaatat
    11281 tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat tggcgatatt
    11341 atccagcgcg cgcgggcaaa atatgacacc acgcgcccgt cgctgcgtaa ttttgtgttg
    11401 agtcataccg acctgatggg tagcgtttcc acgccgttcg caccaatcgt caacaccgct
    11461 acctcgctga aaccggtgcg gcagctgctt gatgcggcgt taaaaatcga tcatcgtcgc
    11521 acgctaccga aatactcctt cggcacgttc cgtcgctggt atcgcagcat agcggctcag
    11581 caagcacaat ataaagacca ggtcgctttc tttcacggat gcttcgttaa ctacaaccat
    11641 ccgcagttag gtaaagattt aattaaagtg ctcaacgcaa tgggtaccgg tgtacaactg
    11701 ctcagcaaag aaaaatgctg cggcgtaccg ctaatcgcca acggctttac cgataaagca
    11761 cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt aaaaggcatt
    11821 ccggtgattg ccacctcctc aacctgtaca tttgccctgc gcgacgaata cccggaagtg
    11881 ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcaacccg ctggctgtgg
    11941 cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa agtggtttat
    12001 cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga gctgttgcgt
    12061 aaaatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat tgcgggtact
    12121 tacggtttca aaaaagagaa ctaccccacc tcacaagcca tcggcgcacc actgttccgc
    12181 cagatagaag aaagcggcgc agatctggtg gtcaccgact gcgaaacctg taaatggcag
    12241 attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctactggc ccaggcgctg
    12301 gcttaaactc ctttctgatg cccggtaagc atgtggttac cgggcatttt tgcgtacacg
    12361 attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa cgtctcgcaa
    12421 aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt catcataaat
    12481 atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga tgcggtattt
    12541 aaaaccttta tgttcacacc cgaaaccgca cgggattttc tcgaaataca tttaccagaa
    12601 ccactgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag ttttattgaa
    12661 aaaagtttac gcgcttacta ctcggatgtt ttgtggtccg tggaaaccag cgacggtgac
    12721 ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat ggcttttcgg
    12781 ctaatgcgct atgccactgc cgccatgcag cgtcacctgg acaaaggcta tgacagagtc
    12841 ccgctggtgg tgccgttgct gttttatcat ggcgaaacct cgccttaccc gtactcactc
    12901 aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac cgaagctttt
    12961 ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg gcgtatagct
    13021 ctgctggaac tgattcaaaa gcatattcgc gaccgcgatt taatcggcat ggtcgacagg
    13081 atcaccacgc ttttggttag aggcttcact aatgacagcc agctacaaac actgtttaat
    13141 tatctgctgc aatgcggcga tacctcccgt ttcacccgtt ttattgagga gattgccgaa
    13201 cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg gcaggaaggg
    13261 catcaaattg gctggcagga aggtatgcat gaacaagcca ttaaaattgc tttgcgcatg
    13321 ctggagcagg gctttgaacg tgagattgtg ctggcgacaa cccaactcac tgatgctgat
    13381 attccgaact gtcattaaaa ggagagatta atgactattg ctgaacggct gcggcaggaa
    13441 ggacatcaaa ttggctggca ggaaggtaaa ttagaaggtt tgcatgaaca agccattaaa
    13501 attgctttgc gcatgctgga acagggcttt gatcgtgacc aggtgctcgc ggccacccag
    13561 ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca cagccgatcc
    13621 ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg cctgatttaa
    13681 acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg ccaacagcga
    13741 caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca gccagaaaac
    13801 cagccgcttt acccgcagca cggatccgcc gaatactggt ttcaataatt cgctgcactt
    13861 ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgca ggtccaataa
    13921 acacgccatc aatcccttcg acgtcgagga tttcgtccag gttatccagt gccgttttac
    13981 tttccacctg caccaacaga caaagcgaat cgttaacttg cgccatgtaa ttctcaatgc
    14041 gtccccagcg cgcagcccgt gccacactgg ccccgacacc acgctcaccg tagggaggat
    14101 agcgcgtggc agacaccacc tgacgtgcct gttcggcagt atcgaccatc gggatcagta
    14161 gagtttgcgc gccaatatcc aggacttgtt taatcagcgg tttactgcct tccaccggac
    14221 ggatcacggg ttggctggca tagggcgcta ccgcctgtag ctgatgataa agatcctgaa
    14281 tggtgtttgg cgcgtgctcc ccgtcaatca gcaaccagtc ataaccagaa gtggcggcaa
    14341 tttctgccat ataggcagtc gttgagctta accacagacc aatttgcact tcgcccttgc
    14401 gtaaacgttc tttaaaggga ttgcttaata atgcgttcat cgtgttcctt aacctattta
    14461 atgatgtgcc acgtcggtct gcgcaacagt gcgatttaca cgcagagtga aaataatcag
    14521 cgaaccgatg accgccaccg ctgccagcgt cagtaatccc gccgcatcgc tggcaaataa
    14581 cgtttctgct ttcacgcgca ggatcggggc aataaacccg cccactgcac caaacaggtt
    14641 tacaaaacca attcccgcag ccagcgccgt accggaaagc agttgtgtcg gcatcgtcca
    14701 gaacactggc tgcacggcaa taaacccgat agctgcaaca cacagcgcta cgatcgccat
    14761 tactggagaa agcagcccga acagaccaat accaatgccc gccgccagta atgtcagcgc
    14821 tgcgacatta cgccgttcgc cggttttatc ggaatagcgc ggaataagcc aggtcccaaa
    14881 caaggccgca acccacggaa tggcggtgac caccgacgct gtaaagccca cttttgttcc
    14941 cagcaatgcc gcaacctggg tcggcaggaa gaaaattaat ccgtaaaccg ccacctgaat
    15001 ggtcaggtag ataatcgcta gttgccagac tcggccatta cgcagcgcat cgctcagccg
    15061 agaagtcact ttctgttgtt cttcacttgc cagttgattg ataagcaacg ttttttcttg
    15121 tttactcaga aaacgtgcct gctccggtgt gtcatcaagc caaaagaatg tgaatacccc
    15181 agcaccgact gccaacaatc cttcaatcac aaacatccag aaccagccgg gatgccccat
    15241 aaatccatgc atctccaaca gcgcgccgga aagcggtgat cccagtgtta acgccagcgg
    15301 tgcgcccata tagaacagcc ccataatgct ggcgcgatta cgctgcggaa accattgcga
    15361 ggtgagataa atcataccag ggaaaaatcc ggcctccgca gcaccaagca gagtgcgaac
    15421 tatcagaaat ttcgcttcag tatcggccca tgccatggct gccgaaagaa atccccacag
    15481 cagtgttgtc gtaccaatcc aggttctggc ccccagtttg cgcatcaaaa gattcgccgg
    15541 aacacccaga aacgcatata ccacaaagaa aatgcctgct cccagcgcat aagcttcatt
    15601 actcaacccg gtatcaatct ggtaggtctg tttggcaaaa ccgatattcg aacggtcgag
    15661 aaacgccagc acatacagcg ccaacataaa cggaattaaa cgcacacggt ttttcttcac
    15721 cacggcgtca agcaaagcgg tgctcataaa aagctcctta gaatatgtgg gcgttaccgt
    15781 tgtcacagca acgcccggag tgattagtgg ctgtaggggc gtttcaaatt gcagtcacga
    15841 ttgagttcga cgccaaaacc gggtttatca agcactgatt tatgaatacg accattcacc
    15901 ggaaccggct cattgagcag aatcgggtca aactgcggac gcatcgttga acaatccggg
    15961 ctggtcatca ggaattcgct gaatggcgta ttggtgaagg tgatcaccgc atggtgagag
    16021 taaacagacg aaccgtgcgg caccaccagt tgccccctgg atttggcgat tgcggcaatt
    16081 tccaccagcg tggttaaacc accgcaccag ccaacatccg gctgcataat gtcgataccg
    16141 gtttctgaaa gcgtacgaaa agattgcagt gtgccgtggt gctcaccgct ggtgaccatc
    16201 attccgactg gcgcgttgcg tttcagttcg cgataacttt catactgctg tggcggcagg
    16261 cactcttcga tccatttcag gttatagggc gcgcaagcgt gggccagttt ggtcgcatag
    16321 ttcacgtcct gactcatcca gcagtcgagc attaaccaga aatcctcacc gcatttttca
    16381 cgcatatccg cgaccatagc ggcatctttg cggatccccg catcgccatc atgtggcccc
    16441 cagtgcgtcg gcattttgcc accgataaag cccatctctt ttgccagatc cggacgcgca
    16501 cctgtggcgt agaactgaat ctcatcacga acagcgccgc ctaaaagttt ataaaccgga
    16561 agcccgacca ctttgccgaa cagatcccac agagccagat cgacacaaga aatcgtattc
    16621 atcaccaggc caccagagcc ggagtaatac agggtggcac tgagcatttg atcgtggatc
    16681 agtttgatat cactgacaca tttaccctca atgaaacggt taagatgttt ttcgacaata
    16741 aaacagccca tttcaccggc tgtcgaaacg gcgaatccgg tctggccgtt ttctgcttcg
    16801 acttcaacaa ccaacgtgcc aagaacgtta atgccaaatg actggcgtga ctgctcgtaa
    16861 tcgcggtatt tactcatcgg ggtggcaata tgatcgtcta tccagtgatt cgccccctgg
    16921 tcgtgataat caccgccgcc agcgcctttt tctgctgtcg caccgccagt aaaccaggcg
    16981 cgaacctgtt taatttttgg tagggtcatg atgttctcca ttgttatgag gcttgtaagt
    17041 caaagggact tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc
    17101 ccgccagata atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg
    17161 cagcggtaag ctcgttattg acgttaaaca ccggcgcagc gacacaacgg acatcggcgt
    17221 aatcttcgcc gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa
    17281 gttgctgtgg atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa
    17341 tactttgctg taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat
    17401 aaagcgaaag gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt
    17461 tcaaaatgta ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg
    17521 tatccatcag ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg
    17581 catgaccgga aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc
    17641 taagaaaacg ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc
    17701 ccaatgtgtc aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa
    17761 tatcaatagc ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc
    17821 ttagatactg gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc
    17881 ccgctcctcg tccagcagac tggagaaatt acgcacaaat ttgtgaatca gcgcacggat
    17941 tttttcttat tgagtacaac aggttggaca aattttgccg gagggggaat gtgccggtgc
    18001 agtaaaccgg cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa
    18061 tccagccatg ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg
    18121 ccatcattgc gcacacttcc tgacggatag ccaggctgta gcgcgtagtg ctgaaacgca
    18181 cacgcagagc gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga
    18241 aacccgaaac agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt
    18301 gcgcagtttg cgccagggtt tcctcctgtg aaggaaccac ttcacacgcc agcaatggag
    18361 cacctgcgcg agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca
    18421 ggctgaactg gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcagtgcctt
    18481 cgaaaatcac gctctgtccg gcaacacgtt taacatccag ccacagtttt tccatcgcct
    18541 gttgctcgct tgccggtccg gtgagtttca gttcgatgat aggcattgag gagcgatagc
    18601 ccattgttac gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg
    18661 aacgaccaaa agtagtcaaa cgcagacaaa ccggcggctg cggtaaagaa aaacgctcgc
    18721 gcaggcgcgg caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag
    18781 taaagaacat caggcaacga ttaagctgca cggcaaaacc acaggcggtg cctaccgggt
    18841 tattgataaa ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta
    18901 ctcgtccacg ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca
    18961 ggccttcacc ttttgctgtc gcagcggcga gtgcgcttaa atcatcgctg gtcggtccca
    19021 gcccgccgtt aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta
    19081 agtcatcaag gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga
    19141 aaaaatcggc cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc
    19201 cggtggataa catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta
    19261 taacgcaaag ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg
    19321 gaagggatca gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc
    19381 ggttaccgtc tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggac
    19441 gcataatggt gtaacgcgcg ccagcattcg cttcttcata actttgaata ccgctggaga
    19501 gcgaatccgg agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact
    19561 gcaaaccacc tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa
    19621 cgccttttcc gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca
    19681 cgccagcgac atcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg
    19741 tcgtttcagt cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga
    19801 tgctcattgc attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa
    19861 tttttttcat tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt
    19921 gtactgcctg gcacagagga ttcaatgagc gaaaaaaagg atctagtcac ttattgtaac
    19981 taaatacttc ttaaataatt aaatgccgaa aaaataaaca cactttatcg atacacacaa
    20041 aaataataat aagtacagtt atcatcgcaa aagagattac tgtcactttc acatatttgt
    20101 gagttaccgt attctcatgg tttcttttta tttataagga atatccctgt gcgacaacgg
    20161 actattgtat gccctttgat tcaaaatgat ggtgcttatt tgctgtgtaa aatggccgac
    20221 gatcgcggcg ttttccccgg tcaatgggcg atttcgggtg gcggcgtgga gcctggcgaa
    20281 cgaattgaag aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg
    20341 acagaaatca cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt
    20401 cgcaaggaag agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa
    20461 gtgaaaataa acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat
    20521 tatgatttga atgtcgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga
    20581 tgacagccat cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga
    20641 taaactttgc ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc
    20701 gcatcacgct tatctttagc aatatatgtc aggcaatgat tatgggtaaa aataacgata
    20761 tttttatcag gtgctttgct ttgtaagtct ttaattgcac tatagatctc attaccgcat
    20821 tgaagaagtc gtttatctac cgtgagcttt ttacccgctg aaaaccaggt cgcggactga
    20881 atggtccgga cggtattact ggaataaaga tcgaaatcag ggatatcagc actaaaagcg
    20941 ttgcccagtt cacgggcatc ctgggtacct ttgaccgtaa tacctgtttt atctgacaag
    21001 cattgattgg ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc
    21061 tgctgtgcca gtctggccag cgttttattg tcgatacgtg gcaaaccatt gctactccag
    21121 gcggcatgag tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gatatatttt
    21181 tttgacttca acgaagagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc
    21241 aaccagacaa ttaccgtgaa attgagctac atttctggcg ataattcgca gttggtgtaa
    21301 tattaaaaat cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa
    21361 ataagaaaaa atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt
    21421 taatattcta tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag
    21481 caaatataag tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct
    21541 ttttcgcgac cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc
    21601 ggttggatca caaccggtcc gaagaatcag gcgcttgagc aagctttttg ccagttgacg
    21661 ggaaatcagc atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg
    21721 gcgttgaaaa ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc
    21781 ctcaatatga tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg
    21841 ctgatggtca cgcctgaagc tatcgagtca gccattacgc cacgcactaa agccatcatt
    21901 ccggtgcatt atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt
    21961 tacggcatcg cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga
    22021 catattggcg caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt
    22081 gctgaaggtg gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg
    22141 aaatttcacg gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag
    22201 gctgaagtct taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc
    22261 ctgacacagt tagtcaaatt agagcacctc aacacccgtc ggcgcgaaat tgcccagcaa
    22321 tatcagcaag cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac
    22381 gttcacgcct ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc
    22441 gatgcgttga tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc
    22501 gctcacacac aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa
    22561 tggaatagcg aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc
    22621 gaccatgtca tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac
    22681 cctgttaaga aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa
    22741 ttaatcaggc gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg
    22801 attgatgacg gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag
    22861 aacagccata ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg
    22921 gcgggattca gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat
    22981 ccgccagaag aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg
    23041 actgtacgcc agaaccgcca ggacagctgg tttcgtaaaa ccgcttcgaa gatgattaac
    23101 cggcttattc agcgcaccac tggcaaagcg atgggtgatt acggttgtat gctgcgcgcc
    23161 tatcgccgtc atattgtcga tgcgatgttg cactgccatg aacgcagcac ctttatcccg
    23221 attctggcga atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt
    23281 gagtttggtg aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg
    23341 acctgcctta ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt
    23401 ggaggtttta gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg
    23461 gcggcagaag gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt
    23521 atcggcatgg gattactcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc
    23581 ccccgctatt ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa
    23641 ccgtcgtttt tgcctaccac gatatgggat gcctcggtat tgaagccctg ctggctgccg
    23701 gttacgaaat tagcgccatt tttacccata ctgataatcc cggtgaaaaa gccttttatg
    23761 gttcggtggc tcgtctggcg gcggaaagag gcattccggt ttatgcgccg gataacgtta
    23821 atcatccgct gtgggtggaa cgcattgccc aactgtcgcc agatgtgatt ttctcttttt
    23881 attatcgcca tcttatttac gacgaaattt tgcagctcgc tcccgcaggt gcatttaatc
    23941 tgcatggttc gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtca
    24001 acggtgaaac ggaaactggc gttacattgc accgaatggt gaaacgtgcc gatgccgggg
    24061 ccattgtggc gcaactgcgc attgccattg cgccagacga tatcgctatt acgctgcatc
    24121 ataaattgtg ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg
    24181 gtaatattct ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt cgcagaacgc
    24241 cggatgacag tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg
    24301 ccgttgccga tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct
    24361 ggtcgtcgcg tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg
    24421 cgccactgct gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg
    24481 acggcattac tatgcagggc tcgcaattag cgcagacgct gggcctggtg caaggttcac
    24541 gcttgaatag ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg
    24601 tgaatggctt tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag
    24661 tttacggtct ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc
    24721 actttgttga aggcgatatc agtattcatt ccgaatggat tgagtatcat gtcaaaaaat
    24781 gtgatgtcgt cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc
    24841 tgcgcgtatt tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgtgaagt
    24901 accgtaagcg aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat
    24961 acttcgatga ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt
    25021 attcggtatc aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt
    25081 tacagttcac cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg
    25141 cagcgcgaat tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt
    25201 caccgattaa gctgattgat ggcggaaaac aaaaacgctg ctttactgat attcgcgatg
    25261 gtatcgaggc gttataccgc attatcgaaa atgcgggaaa tcgctgcgac ggtgaaatta
    25321 tcaacattgg caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg
    25381 cgagcttcga aaaacatccg ctgcgccatc atttcccacc gtttgcgggc tttcgcgttg
    25441 tcgaaagtag cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca
    25501 tccgcaatgc ccaccgctgc ctggactggg agccgaaaat tgatatgcag gaaaccatcg
    25561 acgaaacgct ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa
    25621 agtaggctta cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccgcgtct
    25681 gctggaaatc ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga
    25741 caatatgggc cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg
    25801 ctcaaacgcg gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg
    25861 taaagagatt ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt
    25921 cggcctgcac gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca
    25981 aacaatgatc gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc
    26041 ggtaacctgt tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga
    26101 agcgttccat ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccattgct
    26161 cgaatcagga aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt
    26221 gattggtcgg gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg
    26281 agataaaggc acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca
    26341 taattttgtg gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag
    26401 cgaactgtta tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg
    26461 tgaaggctgg ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac
    26521 cttatcggcc tcttcgcatt tattgcctgc tattacctgt taccgatcag cacgcgtctg
    26581 ctctggcaac ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc
    26641 gactggattg agccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac
    26701 tggattaaca gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc
    26761 gttatctttg cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg
    26821 cgcgataaac gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat
    26881 gccatcggca cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg
    26941 tgcagcttct ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta
    27001 ctgctgggaa tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg
    27061 ccggtattaa gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt
    27121 tacggctggc tggcggttat cagttgcgta ctgacggttc tcccttgggg actggcgata
    27181 gcgcagcggg agcctaactt ctggcactat tttttctggg ttgagcatat tcaacgcttt
    27241 gcactggatg atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc
    27301 ggtagcctgc cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc
    27361 aagcattccg caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc
    27421 gtcgctaaag gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg
    27481 atggcgcatt acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc
    27541 tggattaaca tcgcttttgg cgtcactggc attattgcca catttgtggt ctccccgtgg
    27601 ggaccaatga acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg
    27661 tcgatttttt cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc
    27721 tggccttttg ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct
    27781 gacagagtta tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt
    27841 cagccaagcc gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc
    27901 ctgcaacgcg atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat
    27961 tatccggatg cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa
    28021 catcgtcagg aggggattat tactctcgtg ctttcggttg accgcgatga agatatcaac
    28081 agtctcgcca ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag
    28141 tatcgtccca aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc
    28201 agttgtgtca gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg
    28261 tgctgtggct gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg
    28321 tcttgcagaa cgtaccggta ggcattgctt acccgatgtt aagtctgaat tttgtctggg
    28381 tgacgctggc tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg
    28441 tggcgttcat tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg
    28501 gggattattc agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag
    28561 tcatctgccg ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct
    28621 ggatgcccgg atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta
    28681 taaaacgctt cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt
    28741 gctggtatgg attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc
    28801 actactggga gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa
    28861 acaacgttac tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata
    28921 actgcttgcc gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc
    28981 attctgcaaa ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc
    29041 gatcattttt atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt
    29101 attgcagcac gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt
    29161 cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa tggcagaaat
    29221 gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt agcgcctgac
    29281 gtgaaatttt aataccgccg tttttcagct ccggcggcag agttagccag cgcaccggtt
    29341 gttgaaaacg ggccagctta tctttcaccc attcactaag atcaacgctc tcgtggtcat
    29401 actccatcac cgccaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca
    29461 cctgcaaaac cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct
    29521 ctccgccact gaagaataga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca
    29581 tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc
    29641 cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga
    29701 ttttcacttc ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg
    29761 cacacaccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct
    29821 cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca
    29881 gggaaacgga actacggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt
    29941 gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgtaccgtc atccgcgcac
    30001 cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga
    30061 gcaaccaatc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa
    30121 gatgggcctg ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg
    30181 tcattgagca cagacgcgtc ggctgccacg tagcggcatg tgcgccttca accagctgaa
    30241 tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc cggcaccaga gcaaattgca
    30301 gcgtcagatt gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca
    30361 acacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc
    30421 acgcccgcaa catcacgccg ctgccctcaa ccaccccctg caccgcaaat ccggaggcta
    30481 attcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg
    30541 cgatggtttc tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa
    30601 caaccgctcc agtgcatcaa cttccacgac aggcagcgtg ctacccggcc agcgacgtac
    30661 ctgctgcgcc tgcatcagat ccagcgtgtc cagccctgga atggtgtccg gcgttaacca
    30721 ggcggcaatc cgcgccagtt gcgttaagcc taagctcgat tcaatggaag aactgatcac
    30781 cgccgtcagc cccagcgcgt gcgccgcctg tacctgctcg cgtacttttt ccagactgcc
    30841 cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc
    30901 cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg
    30961 cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtcgc gataatccgg
    31021 gttaacgtat ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag
    31081 acgcaaatgc agatccggaa ttgcctccag caacagattc accaccatgc cgtcgcgcac
    31141 cgcttcgtac aatcccactt tgaccttcgc cactttctcg cctggcatat ctgcaagttt
    31201 gaggatcaga tcgtccggat cgccattaca cagcggtgcc gcacggtagt tggctgcttg
    31261 cggcaacgta tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca cggaaggcat
    31321 ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca gcacactttg
    31381 cgcctcttcc caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc
    31441 gcgctcgcct tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg
    31501 cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac
    31561 ggattccgtt tgaatttgct gaagtcaggc tgacgtttct ggttgaaggc gttgcgacct
    31621 tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcagc
    31681 cccgcctgcc cgtcacagtc ggcgttcagt gcagctttca ggcagcgcag cgccatcggg
    31741 ctgttttgca gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt
    31801 accacggtgt tcacaaggcc catatccagc gcctgttttg cgtcgtactg acggcacagg
    31861 aaccagattt cacgcgcttt tttctgcccg acgatgcgag ccatgtagga agcgccccag
    31921 ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg
    31981 atagtcaggt cgcacatcat gtgcagaacg tgaccgccgc cgatggagta gccagccacc
    32041 atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacattcagg
    32101 tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca
    32161 ccggagcaga acgctttatc gcctgcacca gtcagaatga tcacgccgat gttgtcgtca
    32221 taacgcgcat ctgccagcgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg
    32281 cgcacctgcg gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga
    32341 atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca
    32401 tcaggataaa tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga
    32461 ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt
    32521 cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca cataaataat
    32581 aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag
    32641 tcgcctccag catggcggca agcgttgcgc cattattgtt gctgcgcagc gccaccagct
    32701 cccggcgttg atcgtcattg agtgaggcaa aaacaggctg ttgataccag tcggcaaata
    32761 ccgccgttaa cggttctgtg agaaaacgct gcacccattg gcgatcggaa cgctgacgtt
    32821 ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgacaata accccacaaa
    32881 gccccgccag cccctggcaa gccgccatca tcgccacccg tccaccaagc gagtacccca
    32941 ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag tcggtgacat
    33001 catcaaatcc atcgacgcta atcgccgccg aaccaccgtg acctgggaga tcaacataca
    33061 accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa
    33121 aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc tgcgcgtgca
    33181 ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc
    33241 gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg caaatgccgt
    33301 ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg
    33361 ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt gcggcgttgg
    33421 caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca gcggcgcaga
    33481 aacctgacac aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat
    33541 cgccagcgtc ggtttgccgc ttgcccgctg aacgccggcg gcggtcgaaa gcagcccgtc
    33601 gataccgctg gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc
    33661 atcaatcaga cgtaccacca ggctgttacc aacaaacaat tgcccctgtt caggcagata
    33721 gtcgcagatg cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg caataaccgc
    33781 ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc
    33841 cggatgcagc tccagccagt cggcaatatt ggcaattaag cgacgtccgc gatggtgtgc
    33901 cggatcaagt cgcccttcaa tgtcatcaac aatccagtac tcttctggtt cacagcttgc
    33961 ctgccattgc aggagccgtt tccccgtcag gctgcttccc agttgcacca caatttgcgc
    34021 ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac acggcagcgg
    34081 ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt gcgcccacag
    34141 ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgcttttg
    34201 tcgccagaag aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca
    34261 cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc cggtatcgtc
    34321 catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc ccgcatgaag
    34381 cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg
    34441 gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct ggcgaattgc
    34501 ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga gaatcagttt
    34561 ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg ccgtgccgga
    34621 ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc
    34681 caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa
    34741 cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc cgtgacgcgt
    34801 taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt
    34861 gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa
    34921 tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcagc cctgccgctt
    34981 tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag
    35041 catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc
    35101 tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt
    35161 gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct
    35221 gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag atacagcggc
    35281 gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg
    35341 tatcggcctg taatcgttga cagatatctt ccaccaccag catgttctcg cgctggtttt
    35401 tatcatccgc catcagccac tctcctaact gctgcgcctg cttatcatca ggattatttg
    35461 ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt
    35521 ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat
    35581 tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat
    35641 gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg
    35701 ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg
    35761 tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata aattcttttg
    35821 cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt
    35881 taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc gacgggtcaa
    35941 aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt
    36001 gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacgacagct tcttcatcac
    36061 cattacgttg ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg
    36121 catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg
    36181 gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc
    36241 ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc
    36301 ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg
    36361 cgcccacacc aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg
    36421 cctgcttcgc ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat
    36481 ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag
    36541 aggagcggag cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg
    36601 tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat
    36661 agacaagggt tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac
    36721 catcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca
    36781 gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgta catgtttcga
    36841 gtgttttact catcagctgc tgccccactt tttcgccacg caacgcttca ctgacaatca
    36901 cccgacctat aacgaccggc tcaagatcat catcactttt cagaatcctc gcatacgcca
    36961 ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat
    37021 cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata
    37081 aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat tcaatcataa
    37141 tgctctcctt ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac
    37201 tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca
    37261 cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg
    37321 gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa ctggacaaga
    37381 tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg ctgtgcagtc
    37441 gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat
    37501 ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg
    37561 aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccaatgg
    37621 aacacccact ggaatgctat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa
    37681 atgcccaggc attaaaagct gctggcgtac cgcctggccc actgtttcag gaattaaaag
    37741 cgggcaaaac aatcatgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg
    37801 ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac
    37861 ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg
    37921 aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg
    37981 aggctggagt cggtaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt
    38041 gtcagcacct gttacgtgaa tgtcggtcaa ttttcccggc gactgaactg gcgaatgatt
    38101 tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac agggcgaaac ttgccctgtt
    38161 atcgcaaccc gcgctacata tatccaccca cacgggcata ttgagcgatt tttgatattt
    38221 attcatctcc ttacgcacta tattcagagc aataagttgc ctctttttat cttcattcag
    38281 cacaaccttt gcaactaaaa ggagcctatg atggttacag ttgtcagcaa ttattgtcaa
    38341 ttatctcaaa cccaactcag tcagacattt gcagaaaaat ttactgtgac cgaggaatta
    38401 ctgcagtctt taaaaaaaac agcgttatcc ggggatgaag aaagtattga gttactgcat
    38461 aacattgcgt taggttatga tgaatttgga aaaaaagctg aagatattct ttaccatatt
    38521 gttagaaacc caacaaatga gaccctatcg attatccgac ttattaaaaa tgcctgttta
    38581 aaattatata atttggcaca taccgcaacc aactcccccc ttaaaccaac tgctccggat
    38641 aattcagatg atctcctgtt caaaaagctg tcctcccctt cgaaattaat gacaattatc
    38701 ggtgatgaaa ttcctcttat atctgaaaaa cagtcgcttt cgaaggtgct tttaaatgat
    38761 gagaataatg aattgagtga tgggacaaac ttctgggata aaaatcgtca attaaccaca
    38821 gatgaaatag attgctatct tcagaagatc gccgctaatg caaaaaatac tgaagtcaat
    38881 tatcctactg gtttctacct ctccaattcc aacagcactt acctagaaat cgctctcaat
    38941 gacaatatta agagcgatcc atcatggccg aaagaagtcc agttattccc cataaatact
    39001 ggcggacact
//
LOCUS       DAZFZC010000037        38537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFZC010000037 DAZFZC010000000
VERSION     DAZFZC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.037"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            315..1883
                     /gene="cydA"
                     /locus_tag="ACHKXP_002235"
     CDS             315..1883
                     /gene="cydA"
                     /locus_tag="ACHKXP_002235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO7647233.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1899..3038
                     /gene="cydB"
                     /locus_tag="ACHKXP_002236"
     CDS             1899..3038
                     /gene="cydB"
                     /locus_tag="ACHKXP_002236"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7647234.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3053..3166
                     /gene="cydX"
                     /locus_tag="ACHKXP_002237"
     CDS             3053..3166
                     /gene="cydX"
                     /locus_tag="ACHKXP_002237"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO7647235.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3166..3459
                     /gene="ybgE"
                     /locus_tag="ACHKXP_002238"
     CDS             3166..3459
                     /gene="ybgE"
                     /locus_tag="ACHKXP_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO7647236.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3609..4013
                     /gene="ybgC"
                     /locus_tag="ACHKXP_002239"
     CDS             3609..4013
                     /gene="ybgC"
                     /locus_tag="ACHKXP_002239"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7647237.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4010..4702
                     /gene="tolQ"
                     /locus_tag="ACHKXP_002240"
     CDS             4010..4702
                     /gene="tolQ"
                     /locus_tag="ACHKXP_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO7647238.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4706..5134
                     /gene="tolR"
                     /locus_tag="ACHKXP_002241"
     CDS             4706..5134
                     /gene="tolR"
                     /locus_tag="ACHKXP_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO7647239.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5199..6464
                     /gene="tolA"
                     /locus_tag="ACHKXP_002242"
     CDS             5199..6464
                     /gene="tolA"
                     /locus_tag="ACHKXP_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO7647240.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6597..7889
                     /gene="tolB"
                     /locus_tag="ACHKXP_002243"
     CDS             6597..7889
                     /gene="tolB"
                     /locus_tag="ACHKXP_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO7647241.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7924..8445
                     /gene="pal"
                     /locus_tag="ACHKXP_002244"
     CDS             7924..8445
                     /gene="pal"
                     /locus_tag="ACHKXP_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO7647242.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8455..9246
                     /gene="cpoB"
                     /locus_tag="ACHKXP_002245"
     CDS             8455..9246
                     /gene="cpoB"
                     /locus_tag="ACHKXP_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO7647243.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9411..9486
                     /locus_tag="ACHKXP_002246"
     tRNA            9411..9486
                     /locus_tag="ACHKXP_002246"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9444..9446,aa:Lys,seq:ttt)
     gene            9622..9697
                     /locus_tag="ACHKXP_002247"
     tRNA            9622..9697
                     /locus_tag="ACHKXP_002247"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9655..9657,aa:Val,seq:tac)
     gene            9700..9775
                     /locus_tag="ACHKXP_002248"
     tRNA            9700..9775
                     /locus_tag="ACHKXP_002248"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9733..9735,aa:Lys,seq:ttt)
     gene            9827..9902
                     /locus_tag="ACHKXP_002249"
     tRNA            9827..9902
                     /locus_tag="ACHKXP_002249"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9860..9862,aa:Val,seq:tac)
     gene            9906..9981
                     /locus_tag="ACHKXP_002250"
     tRNA            9906..9981
                     /locus_tag="ACHKXP_002250"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9939..9941,aa:Lys,seq:ttt)
     gene            10128..10203
                     /locus_tag="ACHKXP_002251"
     tRNA            10128..10203
                     /locus_tag="ACHKXP_002251"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10161..10163,aa:Lys,seq:ttt)
     gene            10336..10411
                     /locus_tag="ACHKXP_002252"
     tRNA            10336..10411
                     /locus_tag="ACHKXP_002252"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10369..10371,aa:Lys,seq:ttt)
     gene            10689..11733
                     /gene="nadA"
                     /locus_tag="ACHKXP_002253"
                     /pseudo
     CDS             10689..11733
                     /gene="nadA"
                     /locus_tag="ACHKXP_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
     gene            11771..12490
                     /gene="pnuC"
                     /locus_tag="ACHKXP_002254"
     CDS             11771..12490
                     /gene="pnuC"
                     /locus_tag="ACHKXP_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO7647244.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12487..13428)
                     /gene="zitB"
                     /locus_tag="ACHKXP_002255"
     CDS             complement(12487..13428)
                     /gene="zitB"
                     /locus_tag="ACHKXP_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO7647245.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13542..13922)
                     /gene="ybgS"
                     /locus_tag="ACHKXP_002256"
     CDS             complement(13542..13922)
                     /gene="ybgS"
                     /locus_tag="ACHKXP_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO7647246.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGL
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14238..15290
                     /gene="aroG"
                     /locus_tag="ACHKXP_002257"
     CDS             14238..15290
                     /gene="aroG"
                     /locus_tag="ACHKXP_002257"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO7647247.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15448..16200)
                     /gene="gpmA"
                     /locus_tag="ACHKXP_002258"
     CDS             complement(15448..16200)
                     /gene="gpmA"
                     /locus_tag="ACHKXP_002258"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO7647248.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16402..17442)
                     /gene="galM"
                     /locus_tag="ACHKXP_002259"
     CDS             complement(16402..17442)
                     /gene="galM"
                     /locus_tag="ACHKXP_002259"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO7647249.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17436..18584)
                     /gene="galK"
                     /locus_tag="ACHKXP_002260"
     CDS             complement(17436..18584)
                     /gene="galK"
                     /locus_tag="ACHKXP_002260"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO7647250.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18588..19634)
                     /gene="galT"
                     /locus_tag="ACHKXP_002261"
     CDS             complement(18588..19634)
                     /gene="galT"
                     /locus_tag="ACHKXP_002261"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO7647251.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19644..20660)
                     /gene="galE"
                     /locus_tag="ACHKXP_002262"
     CDS             complement(19644..20660)
                     /gene="galE"
                     /locus_tag="ACHKXP_002262"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO7647252.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20921..22393)
                     /gene="modF"
                     /locus_tag="ACHKXP_002263"
     CDS             complement(20921..22393)
                     /gene="modF"
                     /locus_tag="ACHKXP_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO7647253.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPSEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22461..23249)
                     /gene="modE"
                     /locus_tag="ACHKXP_002264"
     CDS             complement(22461..23249)
                     /gene="modE"
                     /locus_tag="ACHKXP_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO7647254.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23378..23527
                     /gene="acrZ"
                     /locus_tag="ACHKXP_002265"
     CDS             23378..23527
                     /gene="acrZ"
                     /locus_tag="ACHKXP_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO7647255.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23693..24466
                     /gene="modA"
                     /locus_tag="ACHKXP_002266"
     CDS             23693..24466
                     /gene="modA"
                     /locus_tag="ACHKXP_002266"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7647256.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTIK"
     gene            24466..25155
                     /gene="modB"
                     /locus_tag="ACHKXP_002267"
     CDS             24466..25155
                     /gene="modB"
                     /locus_tag="ACHKXP_002267"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO7647257.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25158..26216
                     /gene="modC"
                     /locus_tag="ACHKXP_002268"
     CDS             25158..26216
                     /gene="modC"
                     /locus_tag="ACHKXP_002268"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO7647258.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKDICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26217..27035)
                     /gene="ybhA"
                     /locus_tag="ACHKXP_002269"
     CDS             complement(26217..27035)
                     /gene="ybhA"
                     /locus_tag="ACHKXP_002269"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO7647259.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVIKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27190..28185
                     /gene="pgl"
                     /locus_tag="ACHKXP_002270"
     CDS             27190..28185
                     /gene="pgl"
                     /locus_tag="ACHKXP_002270"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO7647260.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28226..29179)
                     /gene="ybhD"
                     /locus_tag="ACHKXP_002271"
     CDS             complement(28226..29179)
                     /gene="ybhD"
                     /locus_tag="ACHKXP_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7647261.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29363..30415
                     /gene="ybhH"
                     /locus_tag="ACHKXP_002272"
     CDS             29363..30415
                     /gene="ybhH"
                     /locus_tag="ACHKXP_002272"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO7647262.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30491..31924
                     /gene="ybhI"
                     /locus_tag="ACHKXP_002273"
     CDS             30491..31924
                     /gene="ybhI"
                     /locus_tag="ACHKXP_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO7647263.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32107..34368
                     /gene="ybhJ"
                     /locus_tag="ACHKXP_002274"
     CDS             32107..34368
                     /gene="ybhJ"
                     /locus_tag="ACHKXP_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO7647264.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34602..35885)
                     /gene="ybhC"
                     /locus_tag="ACHKXP_002275"
     CDS             complement(34602..35885)
                     /gene="ybhC"
                     /locus_tag="ACHKXP_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO7647265.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36020..37090)
                     /locus_tag="ACHKXP_002276"
     CDS             complement(36020..37090)
                     /locus_tag="ACHKXP_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7647266.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(37068..37286)
                     /locus_tag="ACHKXP_002277"
     CDS             complement(37068..37286)
                     /locus_tag="ACHKXP_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7647267.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(37326..37493)
                     /locus_tag="ACHKXP_002278"
     CDS             complement(37326..37493)
                     /locus_tag="ACHKXP_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647268.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENISDCYDHWMLWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            37736..38338
                     /locus_tag="ACHKXP_002279"
     CDS             37736..38338
                     /locus_tag="ACHKXP_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647269.1"
                     /translation="MSYFLRKKWMVNLSGSGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQRGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPELHEN
                     IRICYPYGNYEYDNIIYVEKKINILGAVVTYAQTARDDTE"
BASE COUNT         9265 a         9361 c        10049 g         9862 t
ORIGIN      
        1 gcatcaaaaa gagataaatt gttctcgatc aaattggctg aaaggcggta atttagctat
       61 aaattgatca ccgtcgaaaa atgcaaattt gcttcaacaa aaacctgttt attgtaagga
      121 ttttgcggcg taatatatac gtgggatcaa tttgagtttt tattaacatg tttgcaacct
      181 ttctttacgc cgtttttgtg tgcattcaca tggtatgatg aaagtgttca aacaaatttc
      241 tattggggca tgcgtgtgac cctttctaac ggggttcact ctcggagtct tcatgcgatg
      301 agcaaggagt catgatgtta gatatagtcg aactgtcgcg cttacagttt gccttgaccg
      361 cgatgtacca cttccttttt gtgccactga cgctcggtat ggcgttcctg ctggccatta
      421 tggaaacggt ctacgtcctc tccggcaaac agatttataa agatatgacc aagttctggg
      481 gcaagttgtt tggtatcaac ttcgctctgg gtgtggctac cggtctgacc atggagttcc
      541 agttcgggac taactggtct tactattccc actatgtagg ggatatcttc ggtgcgccgc
      601 tggcaatcga aggtctgatg gccttcttcc tcgaatccac ctttgtaggt ctgttcttct
      661 tcggttggga tcgtctgggt aaagttcagc atatgtgtgt cacctggctg gtggcgctcg
      721 gttcaaacct gtccgcactg tggattctgg ttgcgaacgg ctggatgcaa aacccaatcg
      781 cgtccgattt caactttgaa actatgcgta tggagatggt gagcttctcc gagctggtgc
      841 ttaacccggt tgctcaggtg aaattcgttc acactgtagc gtctggttat gtgactggcg
      901 cgatgttcat cctcggtatc agcgcatggt atatgctgaa aggtcgtgac ttcgccttcg
      961 ctaaacgctc ctttgctatc gctgccagct tcggtatggc tgctgttctg tctgttattg
     1021 ttctgggtga tgaatccggc tacgaaatgg gcgacgtgca gaaaaccaaa ctggctgcta
     1081 ttgaagccga gtgggaaacg caacctgcgc ctgctgcctt tactctgttc ggcattcctg
     1141 atcaggaaga ggagacgaac aaatttgcga ttcagatccc ttacgcactg ggcatcattg
     1201 caacgcgttc cgtggatacc ccggttatcg gcctgaaaga gctgatggtg cagcatgaag
     1261 aacgcattcg taacgggatg aaggcgtact ctctgctcga acaactgcgt tctggttcta
     1321 ccgaccaggc ggttcgtgac cagttcaata gcatgaagaa agacctcggt tacggtctgc
     1381 tgctgaaacg ctatacgcca aacgtggctg atgcgactga agcgcagatt caacaggcaa
     1441 ccaaagactc catcccgcgt gtagcgccgc tgtactttgc gttccgtatc atggtggcgt
     1501 gtggcttcct gcttctggca atcatcgcgc tctctttctg gagtgtcatc cgcaaccgca
     1561 ttggcgagaa aaaatggctt ctgcgcgccg cgctgtacgg tattccgctg ccgtggattg
     1621 ctgtagaagc gggctggttc gtggctgaat atggccgcca accgtgggct atcggtgaag
     1681 tgctgccgac agctgtggcg aactcgtcac tgaccgcagg cgatctcatc ttctcaatgg
     1741 tgctgatttg cggcctgtat accctgttcc tggtggcaga attgttctta atgttcaagt
     1801 ttgcacgcct cggcccaagc agcctgaaaa ccggtcgcta tcactttgag cagtcttcca
     1861 cgactactca gccggcacgc taagacagga gtcgtcaaat gatcgattat gaagtattgc
     1921 gttttatctg gtggctgctg gttggcgttc tgctgattgg ttttgcagtc actgacggtt
     1981 tcgacatggg ggtgggcatg ctcacccgtt tcctcggtcg taacgacacc gagcgtcgaa
     2041 ttatgattaa ctccattgca ccacactggg acggtaacca ggtttggctg atcaccgcgg
     2101 gcggcgcact ctttgctgcc tggccgatgg tctatgccgc tgcgttctcc ggcttctatg
     2161 tggcgatgat cctcgtgctg gcgtctttgt tcttccgtcc ggtcggtttt gactaccgct
     2221 ccaagattga agaaacccgc tggcgtaaca tgtgggactg gggcatcttc attggtagct
     2281 tcgttccgcc gctggtaatt ggtgtagcgt tcggtaacct gttgcagggc gtaccgttca
     2341 acgttgatga atatctgcgt ctgtactaca ccggtaactt cttccagttg cttaacccgt
     2401 tcggcctgct ggcaggcgtg gtgagcgtag ggatgatcat tactcagggc gcaacctatc
     2461 tgcaaatgcg taccgtgggc gaactgcacc tgcgtacccg tgcaacggct caggtggctg
     2521 cgctggtgac actggtctgt ttcgcactgg ctggcgtatg ggtgatgtac ggtatcgatg
     2581 gttatgtcgt gaaatcgaca atggaccatt acgcagcctc taacccactg aataaagaag
     2641 tggttcgtga agctggcgca tggctggtta acttcaacaa cacgccaatt ctgtgggcta
     2701 ttccggcact gggtgtggtt ctgccgctgc tgaccatcct gactgcacgt atggataaag
     2761 ccgcgtgggc gtttgtgttc tcctccctga ctctggcctg catcatcctg acagccggta
     2821 tcgcaatgtt cccgtttgtg atgccgtcca gcaccatgat gaacgcaagt ctgacaatgt
     2881 gggatgcaac ttccagccag ctgacgctta acgtcatgac ctgggttgcg gtggttctgg
     2941 taccgatcat tctgctctac accgcctggt gttactggaa aatgttcggt cgtatcacca
     3001 aagaagatat tgaacgtaac acccactctc tgtactaagt aaggagctaa aaatgtggta
     3061 tttcgcatgg attctgggaa cgcttcttgc ctgttcgttt ggggtaatca ccgcgctggc
     3121 gcttgaacac gtcgaatcag gcaaagccgg tcaagaagac atctgatgag taagattatc
     3181 gcgactttgt atgcggtaat ggacaagcgc cccctgcggg cgctttcctt cgtgatggcg
     3241 cttctgttag caggatgtat gttttgggac ccatctcgtt ttgccgcgaa gaccagtgaa
     3301 ctggaaatct ggcatggttt attgctgatg tgggccgtct gtgctggtgt gattcacggc
     3361 gtgggctttc gtccgcagaa ggttctttgg caagggattt tttgcccatt gcttgccgat
     3421 attgttctca ttgtcgggct gattttcttc ttcttttaaa tcagaattct ctttaaaaaa
     3481 ttatgggccg ctccaggccc ataaattttt acgctccctt aacttgccct cattcccaaa
     3541 cctcaatcgc gcgcgtatag tagcagcgtt taaaagctct aacttttgtt gcattaccgg
     3601 gatgtaaagt gaatacaacg ctgtttcgat ggccggttcg cgtctactat gaagataccg
     3661 atgccggtgg tgtggtgtac cacgccagtt acgtcgcttt ttatgaaaga gcacgcacag
     3721 agatgctgcg tcatcatcac ttcagtcagc aggcgctgat ggctgaacgc gttgcctttg
     3781 tggtacgtaa aatgacggtg gaatattacg cacctgcgcg gctcgacgat atgctcgaaa
     3841 tacagactga aataacatca atgcgtggca cctctttggt tttcacgcaa cgtattgtca
     3901 acgccgagaa tactttgctg aatgaagcag aggttctggt tgtttgcgtt gacccactca
     3961 aaatgaagcc tcgtgcgctt cccaagtcta ttgtcgcgga gtttaagcag tgactgacat
     4021 gaatatcctt gatttgttcc tgaaggctag ccttctggtt aaacttatca tgttgatttt
     4081 gattggtttt tcaatcgcat cttgggccat tattatccag cggacccgta ttcttaacgc
     4141 agcggcacgc gaagccgaag cgtttgaaga taaattctgg tctggaatcg aactctctcg
     4201 cctctatcaa gagagccagg ggaaacggga taatctgact ggttcggaac aaatctttta
     4261 cagcgggttc aaagagtttg tgcgcctgca tcgtgccaat agccatgcgc cggaagccgt
     4321 agtggaaggg gcgtcgcgtg ctatgcgtat ctccatgaac cgtgaacttg aaaatctgga
     4381 aacgcacatt ccgttcctcg gtacggttgg ctccatcagc ccgtatattg gtctgtttgg
     4441 tacggtctgg gggatcatgc acgcctttat cgccctcggg gcggtaaaac aagcaacact
     4501 gcaaatggtt gcgcccggta tcgcagaagc gttgattgcg actgcaattg gtctgtttgc
     4561 cgctatcccg gcagttatgg cctacaaccg cctcaaccag cgcgtaaaca aactggaact
     4621 gaattacgac aactttatgg aagagtttac cgcgattctg caccgccagg cgtttaccgt
     4681 tagcgagagc aacaaggggt aagccatggc cagagcgcgt ggacgaggtc gtcgcgatct
     4741 caagtccgaa atcaacattg taccgttgct ggacgtactg ctggtgctgt tgctgatctt
     4801 tatggcgaca gcgcccatca tcacccagag cgtggaggtc gatctgccag acgctactga
     4861 atcacaggcg gtgagcagta acgataatcc gccagtgatt gttgaagtgt ctggtattgg
     4921 tcagtacacc gtggtggttg agaaagatcg cctggagcgt ttaccaccag agcaggtggt
     4981 ggcggaagtg tccagccgtt tcaaggccaa cccgaaaacg gtctttctga tcggtggcgc
     5041 aaaagatgtg ccttacgatg aaataattaa agcactgaac ttgttacata gtgcgggtgt
     5101 gaaatcggtt ggtttaatga cgcagcctat ctaaacatct gcgtttccct tgcttgaaag
     5161 agagcgggta acaggcgaac agtttttgga aaccgagagt gtcaaaggca accgaacaaa
     5221 acgacaagct caagcgggcg ataattattt cagcagtgct gcatgtcatc ttatttgcgg
     5281 cgctgatctg gagttcgttc gatgagaata tagaagcttc agccggaggc ggcggtggtt
     5341 cgtccatcga cgctgtcatg gttgattcag gtgcggtagt tgagcagtac aaacgcatgc
     5401 aaagccagga atcaagcgcg aagcgttctg atgaacagcg caagatgaag gaacagcagg
     5461 ctgctgaaga actgcgtgag aaacaagcgg ctgaacagga acgcctgaag caacttgaga
     5521 aagagcggtt agcggctcag gagcagaaaa agcaggctga agaagccgca aaacaggccg
     5581 agttaaagca gaagcaagct gaagaggcgg cagcgaaagc ggcggcagat gctaaagcga
     5641 aggccgaagc agatgctaaa gctgcggaag aagcagcgaa gaaagcggct gcagacgcaa
     5701 agaaaaaagc agaagcagaa gccgccaaag ccgcagccga agcgcagaaa aaagccgagg
     5761 cagccgctgc ggcactgaag aagaaagcgg aagcggcaga agcagctgca gctgaagcaa
     5821 gaaagaaagc ggcaactgaa gctgctgaaa aagccaaagc agaagctgag aagaaagcgg
     5881 ctgctgaaaa ggctgcagct gataagaaag cggcagcaga gaaagctgca gccgacaaaa
     5941 aagcagcaga aaaagcggct gctgaaaagg cagcagctga taagaaagca gcggcagaaa
     6001 aagccgccgc agacaaaaaa gcggcagcgg caaaagctgc agctgaaaaa gccgctgcag
     6061 caaaagcggc cgcagaggca gatgatattt tcggtgagct aagctctggt aagaatgcac
     6121 cgaaaacggg gggaggggcg aaagggaaca atgcttcgcc tgccgggagt ggtaatacta
     6181 aaaacaatgg cgcatcaggg gccgatatca ataactatgc cgggcagatt aaatctgcta
     6241 tcgaaagtaa gttctatgac gcatcgtcct atgcaggcaa aacctgtacg ctgcgcataa
     6301 aactggcacc cgatggtatg ttactggata tcaaacctga aggtggcgat cccgcacttt
     6361 gtcaggctgc gttggcagca gctaaacttg cgaagatccc gaaaccacca agccaggcag
     6421 tatatgaagt gttcaaaaac gcgccattgg acttcaaacc gtaatcgcga tgttgactgt
     6481 tcggacggtc aacatcaggc accggttgcc acggggttct ggtagttttg tgtattttag
     6541 tttgttaaca ttctgctaaa ttatcgtggg ccatcggtcc agataaggga gatatgatga
     6601 agcaggcatt acgagtagca tttggttttc tcatactgtg ggcatcagtt ctgcatgctg
     6661 aagtccgcat tgtgatcgac agcggtgtag attccggtcg tcctattggt gttgttcctt
     6721 tccagtgggc ggggcctggt gcggcacctg aagatattgg cggcatcgtt gctgctgact
     6781 tgcgtaacag cggtaaattt aatccgttag atcgcgctcg tctgccacag cagccgggta
     6841 gtgcgcagga agtacaacca gctgcatggt ccgcactggg cattgacgct gtagttgtcg
     6901 gtcaggtcac tccgaatccg gatggttctt acaatgttgc ttatcaactt gttgacactg
     6961 gcggcgcacc gggtactgta cttgctcaga actcgtacaa agtgaacaag cagtggctgc
     7021 gttatgctgg tcataccgcc agtgatgaag tgtttgaaaa actgaccggc attaaaggtg
     7081 cgttccgtac tcgtattgcc tacgttgttc agaccaacgg cggtcagttc ccgtatgaac
     7141 tgcgcgtatc tgactatgac ggttacaacc agtttgtcgt tcaccgttca ccgcagccgc
     7201 tgatgtctcc ggcgtggtca ccagacggtt ctaaactggc ttatgtgacc ttcgaaagcg
     7261 gtcgttccgc gctggttatt cagacgctgg caaatggcgc tgtacgtcag gtggcttcat
     7321 tcccgcgtca caacggtgca cctgcattct cgccagacgg cagcaaactg gcattcgcct
     7381 tgtcgaaaac cggtagtctg aacctgtacg taatggattt ggcttctggt cagatccgcc
     7441 aggtgactga tggtcgcagt aacaataccg aaccgacctg gttcccggat agccagaacc
     7501 tggcatttac ttctgaccag gccggtcgtc cgcaggttta taaagtgaat atcaacggcg
     7561 gtgcgccaca acgtattacc tgggaaggtt cgcagaacca ggatgcggat gtcagcagcg
     7621 acggtaaatt tatggtaatg gtcagctcca atggtgggca gcagcacatt gccaaacaag
     7681 atctggcaac gggaggcgta caagttctgt cgtccacgtt cctggatgaa acgccaagtc
     7741 tggcacctaa cggcactatg gtaatctaca gctcttctca ggggatggga tccgtgctga
     7801 atttggtttc tacagatggg cgtttcaaag cgcgtcttcc ggcaactgat ggacaggtca
     7861 aattccctgc ctggtcgccg tatctgtgat aataattaat tgaatagtaa aggaatcatt
     7921 gaaatgcaac tgaacaaagt gctgaaaggg ctgatgattg ctctgcctgt tatggcaatt
     7981 gcggcatgtt cttccaacaa gaacgccagc aatgacggca gcgaaggcat gctgggtgcc
     8041 ggcactggta tggatgcgaa cggcggcaac ggcaacatgt cttccgaaga gcaggctcgt
     8101 ctgcaaatgc aacagctgca gcagaacaac atcgtttact tcgatctgga caagtacgat
     8161 atccgttctg acttcgctca aatgctggat gcacatgcaa acttcctgcg tagcaacccg
     8221 tcttacaaag tcaccgtaga aggtcacgcg gacgaacgtg gtactccgga atacaacatc
     8281 tccctgggtg aacgtcgtgc gaacgccgtt aagatgtacc tgcagggtaa aggcgtttct
     8341 gcagaccaga tctccatcgt ttcttacggt aaagaaaaac ctgcagtact gggtcatgac
     8401 gaagcggcat actccaaaaa ccgtcgtgcg gtactggttt actaagagaa ttgcatgagc
     8461 agtaacttca gacatcaact attgagtctg tcgttactgg ttggtatagc ggccccctgg
     8521 gccgcttttg ctcaggcacc aatcagtagt gtcggctcag gctcggtcga agaccgcgtc
     8581 actcaacttg agcgtatttc taatgctcac agccagcttt taacccaact ccagcaacaa
     8641 ctttctgata atcaatccga tattgattcc ctgcgtggtc agattcagga aaatcagtat
     8701 caactgaatc aggtcgtgga gcggcaaaag cagatcctgt tgcagatcga cagcctcagc
     8761 agcggtggtg cagcggcgca atcaaccagc agcgatcaaa gcggtgcggc ggcatcaacg
     8821 acgccgacag ctgatgctgg tactgcgaat gctggcgcgc cggtgaaaag cggcgatgca
     8881 aacacggatt acaatgcagc tattgcgctg gtgcaggata aatcccgcca ggatgacgca
     8941 atggtggcat ttcagaattt catcaaaaat taccctgatt caacttacct gccaaacgcc
     9001 aattattggc tgggtcagtt aaactacaac aagggtaaaa aagatgatgc ggcgtactat
     9061 tttgcttcgg tagtgaaaaa ctatccgaag tcaccaaagg ctgctgatgc gatgtttaaa
     9121 gtcggcgtca tcatgcagga caaaggtgac accgcaaaag cgaaagccgt ataccagcag
     9181 gttatcagta aataccctgg tactgatggc gctaaacagg cacaaaaacg tctgaacgcg
     9241 atgtaatgca taacacacga ccagaagtcg cattatttct ggtcgtgtcg tgcgaatcat
     9301 aagcagttga gtgatctgca tcgaaatttt tgttgcgctc aagtctgaaa tcagtaatat
     9361 atgccgccgt tgccacggga tatcaaacaa accgaaagca acgaaaaagt gggtcgttag
     9421 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
     9481 ccaccaatcg ctaaggtgga agcggtagta aaacgtgaag gataacgttg catcagcaac
     9541 ggcccgaagg gcgagacgaa gtcgagtcat cctgcacgac ccaccactaa catagttagt
     9601 tgtagtatcc agcgtagtat tgggtgatta gctcagctgg gagagcacct cccttacaag
     9661 gagggggtcg gcggttcgat cccgtcatca cccaccaccg ggtcgttagc tcagttggta
     9721 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccactaat
     9781 tacggtgggt tcggtggaag tagtttgtag tatccagcgc agtatcgggt gattagctca
     9841 gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac
     9901 cactcgggtc gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt
     9961 cgaatcctgc acgacccacc agttttaaca tcaaactcag atgttaagcg tgaaggataa
    10021 cgttacgcca gcaacggccc gtagggcgag cgaagcgagt catcctgcac gagccaccaa
    10081 ttttaaagat tggttccgag tagaaatctt tcaggtaaca cccatatggg tcgttagctc
    10141 agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca
    10201 ccaatttaaa ggtggttact ggtagagaac gtgaaggata acgttgcgtt agcaacggcc
    10261 cgaagggtga gacgaagtcc agtcatcctg cacgacccac catcctgaat gattaaagca
    10321 gcataatccc gcaaggggtc gttagctcag ttggtagagc agttgacttt taatcaattg
    10381 gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa aggcgctttt
    10441 ttgctattca ggcatctctc aatttcaatt cttgaccctg atgacatact ccgcgcttgc
    10501 tgcgtaaaca actttacgtc ggaggaataa gacgcaaagt gtggcattcg gcatctcata
    10561 cgtaattcga gtgacttttc cccaccattc gactatcttg tttagcatac aaaacaaatt
    10621 acaccgataa cagcgaatat tacgctaatg tcggttttaa tgttaagcct gtaaaacgag
    10681 atggtaagat gagcgtaatg tttgatccag acacggcgat ttatcctttc cccccgaagc
    10741 cgacgccgtt aagcattgat gaaaaagcgt attaccgcga gaagataaaa cgtctgctaa
    10801 aagaacgtaa tgcggtgatg gttgcccact actataccga tcccgaaatt caacaactgg
    10861 cagaagaaac cggtggctgt atttctgatt ctctggaaat ggcgcgcttc ggtgcaaagc
    10921 atcccgcttc tactttgtta gtcgctgggg tgagatttat gggagaaacc gccaaaattc
    10981 tcagtccgga aaaaacaatt ctgatgccga cacttcaggc tgaatgttca ctggatctcg
    11041 gctgccctgt tgaagaattt aacgcatttt gcgatgccca tcccgatcgt actgtcgtcg
    11101 tctacgccaa cacttctgct gcggtaaaag cgcgcgcaga ttgggtggta acttcaagca
    11161 ttgccgtcga acttattgat catcttgata gtttgggtga aaaaatcatc tgggcacccg
    11221 acaaacatct ggggcgttac gtgcaaaaac agacgggtgg agacattcta tgctggcagg
    11281 gtgcctgtat tgtgcatgat gaatttaaga ctcaggcgtt aacccgcttg caagaagaat
    11341 acccggatgc tgccatactg gtgcatccag aatcaccaca agctattgtc gatatggcgg
    11401 atgcggtcgg ttccaccagt caactgatcg ctgctgcgaa aacattgcca catcagaggc
    11461 ttattgtggc aaccgatcgg ggtattttct acaaaatgca gcaggcggtg ccagataaag
    11521 agttactgga agcaccaacc gcaggtgagg gcagcaacct gccgcagctg cgcgcattgt
    11581 ccgtggatgg ccatgaatgg ccttcaggcc atcgcagagg cattagaaca ggaaggaagc
    11641 aatcacgagg ttcatgttga tgaaaggctg cgagagaggg cgctggtgcc gctcaatcgt
    11701 atgctggatt ttgcggctac actacgtgga taacgaataa taaggcgtaa cgttacgctt
    11761 tgggggaaag atggattttt ttagtgtgca gaatatcctg gtacatatac caataggggc
    11821 aggcggttat gatctctcat ggatcgaagc ggtaggcacg atcgccgggt tgctgtgtat
    11881 tggccttgcc agtctggaga agatcagcaa ctacttcttt ggcctgatca acgtcacctt
    11941 gtttggcatt attttctttc agattcagct gtatgccagc ctgctattac aggtgttttt
    12001 ctttgccgcg aatatttacg gttggtatgc gtggtcgcga caaaccagtc agaacgaggc
    12061 ggagttgaaa attcgctggt tgccattgcc gaaggcactc agctggttgg cggtttgcgt
    12121 tgtttcgatt ggtctgatga cggtatttat caatccggtg tttgcatttt tgacccgcgt
    12181 ggcagtcatg atcatgcaag cattaggatt acaggttgtg atgcctgaac tgcaaccgga
    12241 cgctttcccg ttctgggatt catgcatgat ggtgttatct atcgtggcaa tgattctgat
    12301 gacgcgtaag tatgtggaaa actggctgtt gtgggtgatt attaacgtga ttagcgtcgt
    12361 tatttttgca cttcagggcg tttacgccat gtctctggag tacatcatcc tgacctttat
    12421 tgcgctcaac ggcagccgga tgtggatcaa cagcgcacgt gaaagaggct cacgcgcgct
    12481 gtcccattaa tggtgatgat gtgaatggcc agacacccct tcattcaggt ggcagtctgg
    12541 cccatgacaa ggttgatatt ccatctgaat ggtggcgtgc tcaatctgat agtgatccat
    12601 caggtagtgt tggatctgat ccaacaaggc atcgtgatcg tgtggcggga tcacctgcac
    12661 atgcaacgtc atcaccggct tctcgcctac catccataca tgtacatggt gcacattgcg
    12721 gacttccggg atttcccggc acatacgacg cttcagttca gcgatatcca gcgataccgg
    12781 tgcaccttca agtaattcat tcacgctatc tttcaacagt cgccacgcgc tacgcagaac
    12841 cagaagcgac accagtatcg agagaatggg gtcagcaggt gtccagccag tccagataat
    12901 aatcaacgcg gcaataatag ccccaaccga acccagcagg tccccgagca catgcagtgc
    12961 cgctgctcgt acgttgaggt ttttctcttc gctgccgtga tgaagtaacc agaaagaaag
    13021 tatatttgcc agcaacccag ccacggcaat tgccatcatc atgccgccct cgaccggacg
    13081 tggcgtgcgg aagcgttcta tcgcctccca gacaatcaaa atggtaatta ccacgagggc
    13141 tatggcattc acaaacgccg cgagcgtggt cagtcttagc caaccgaaag tgtggcgaat
    13201 ggtgggagga cgacgtgaaa attgcacggc gagtagggcg aaaagcaggg cggcagtatc
    13261 ggtcaacata tgacccgcat cggccagcaa tgccagagaa ccagaaagaa aaccaccaac
    13321 gacttctacc agcataaacc cggcagtcac gccgaaagca tacaacaagc gacgagcatt
    13381 attatcttct ggcaggtgtg aggacgtgtg tgagtgtgag tgcgccatga cgagtcatcc
    13441 cttaattatt ctcacttaac tttatgacat catactgctt ttagaagtga aaaattaaaa
    13501 gggagagact ccgctctccc attattggct attttgcagg gttactgcgt ggtaccgtcg
    13561 gttttggtat cgacatcatt attgatgcca tcaccggttt gtaccttttt attgatatcc
    13621 ggacagcgac catctttgca catggtgttc ttgtgctctt catctttggt cattccgtca
    13681 ttgttcattg aagaaccatc cgaatgcagc attgtgccgc cagaaccggt atttaccccg
    13741 ttattgtcga cgttatttgg cgcgacattt tcacgggcgt caggggctac caggcccgca
    13801 tcagctgcgg cgtttgcctg gccgttatta gtttgcgctc cgctatcggc agccagtgcg
    13861 gcaccgctgg caaggcttag agtggcagtc agaaataatg tggccagttt tgtcattttc
    13921 ataggatgct cctgttatgg tcgttatgtc ggataacctc ttccaacagt gcatttgcag
    13981 gtgaatataa ggcattggtt taagatttca gccaggttat gaaacgcagc agagaatctt
    14041 gaaataatta acaaacaaag gagttacagt tagaaattgt aggagagatc tcgtttttcg
    14101 cgacaatctg gcgtttttct tgctaattcc aggattaatc cgttcatagt gtaaaacccc
    14161 gtttacacat tctgacggaa gatatagatt ggaagtattg cattcactaa gataagtatg
    14221 gcaacactgg aacagacatg aattatcaga acgacgattt acgcatcaaa gaaatcaaag
    14281 agttacttcc tcctgtcgca ttgctggaaa aattccccgc tactgaaaat gccgcgaata
    14341 cggttgccca tgcccgaaaa gcgatccata agatcctgaa aggtaatgat gatcgcctgt
    14401 tggttgtgat tggcccatgc tcaattcatg atcctgtcgc ggcaaaagag tatgccactc
    14461 gcttgctggc gctgcgtgaa gagctgaaag atgagctgga aatcgtaatg cgcgtctatt
    14521 ttgaaaagcc gcgtaccacg gtgggctgga aagggctgat taacgatccg catatggata
    14581 atagcttcca gatcaacgac ggtctgcgta tagcccgtaa attgctgctt gatattaacg
    14641 acagcggtct gccagcggca ggtgagtttc tcgatatgat caccccacaa tatctcgctg
    14701 acctgatgag ctggggcgca attggcgcac gtaccaccga atcgcaggtg caccgcgaac
    14761 tggcatcagg gctttcttgt ccggtcggct tcaaaaatgg caccgacggt acgattaaag
    14821 tggctatcga tgccattaat gccgccggtg cgccgcactg cttcctgtcc gtaacgaaat
    14881 gggggcattc ggcgattgtg aataccagcg gtaacggcga ttgccatatc attctgcgcg
    14941 gcggtaaaga gcctaactac agcgcgaagc acgttgctga agtgaaagaa gggctgaaca
    15001 aagcaggcct gccagcacag gtgatgatcg atttcagcca tgctaactcg tccaaacaat
    15061 tcaaaaagca gatggatgtt tgtgctgacg tttgccagca gattgccggt ggcgaaaagg
    15121 ccattattgg cgtgatggtg gaaagccatc tggtggaagg caatcagagc ctcgagagcg
    15181 gggagccgct ggcctacggt aagagcatca ccgatgcctg catcggctgg gaagataccg
    15241 atgctctgtt acgtcaactg gcgaatgcag taaaagcgcg tcgcgggtaa ggtttaattg
    15301 tcggatgcgc cgtcagagtg gcgtatccga tgaatcacca caggcctgat aagtcgcgca
    15361 gcgtcgcatc aggcaatgtg ctccattgtt agcaacaaaa aagccgactc acttgcagtc
    15421 ggctttctca ttttaaacga atgacgttta cttcgcttta ccctggtttg caaccgccgc
    15481 tgctttcgct gcgatctcgt cagcattacc cagataatag cgtttcagcg gtttgaaatt
    15541 ctcgtcgaac tcatacacca gcggcacgcc agtcgggata ttaagctcaa gaatctcttc
    15601 ttcgctcatg ttatcaagat atttcaccag cgcacgtaaa gagttaccgt gtgcagcgat
    15661 gatcacgcgc tcaccgctct tcatacgcgg cagaatagtt tcattccagt aagggatcac
    15721 gcggtcaatg gtcagcgcca ggctttccgt cagcggcagt tctttctcgc tcagtttcgc
    15781 gtaacgcgga tcgtgacccg gataacgctc atcatcttta gtcagttccg gcggagtcac
    15841 tgcaaaacca cgacgccact gtttcacctg ctcgtcgcca tacttttcag cagtttccgc
    15901 tttgttcaga ccctgcaacg caccgtagtg acgttcgttc agtttccagg atttctcaac
    15961 gggcagccat gcctgatcca gttcgtccag cacattccac agggtatgga tagcgcgttt
    16021 cagcacagaa gtgtaagcaa agtcaaagct gtaaccttcc tctttcagca gcttacctgc
    16081 tgcttttgct tcgcttacgc ctttctcaga cagatccacg tcgtaccaac cggtgaaacg
    16141 gttttctttg ttccactgac tttcgccatg acgaaccaga accagcttag ttacagccat
    16201 atacttactc ctcaaatcat cttttaatga taataattct cattatattg ccgcgacgaa
    16261 gcaacagcaa tgcttacgca taaccatagc gaaaatagtg gcgcagtgta aggttgttgt
    16321 gaatattgag ttgcaaatat gtcggtgttt gctggtgatt tgaacaatat gagataaagc
    16381 cctcatgacg agggcttaac attactcagc aataaactga tattccgtca ggctggaata
    16441 ctcttcgcca ggacgcagga agcagtccgg ttgcggccat tcagggtggt tcgggctgtc
    16501 cggtagaaac tcgctttcca gagccagccc ttgccagtcg gcgtaaggtt cggttccccg
    16561 cgacggtgtg ccgccgagga agttgccgga gtagaattgc agagccggag cggtggtgta
    16621 gaccttcagc tgcaattttt catctgctga ccagacatgc gccgccactt tcttgccatc
    16681 gcctttggcc tgtaacaaga atgcgtgatc gtaacctttc actttgcgct gatcgtcgtc
    16741 ggcaagaaac tcactggcga tgattttggc gctgcggaaa tcaaaagacg ttccggcgac
    16801 agatttcagg ccgtcgtgcg gaatgccgcc ttcatcaacc ggcagatatt cgtccgccag
    16861 aatctgcaac ttgtgattgc gcacgtcaga ctgctcgccg tcaagattga aatagacgtg
    16921 attagtcata ttcaccgggc aaggtttatc aactgtggcg cgataagtaa tggagatacg
    16981 gttatcgtcg gtcagacgat attgcaccgt cgcgccgaga ttacccggga agccctgatc
    17041 accatcatct gaactcaggg caaacagcac ctgacgatcg ttctggttca caatctgcca
    17101 gcgacgtttg tcgaaccctt ccggcccgcc gtgcagctgg ttaacgccct gacttggcga
    17161 aagcgtcacg gtttcaccgt caaaggtata acggctattg gcgatacggt tggcataacg
    17221 accaatagag gcccccagaa acgcggcctg atcctgatag cattccgggc tggcacagcc
    17281 gagcagcgcc tcgcggacgc tgccatcgga aagcggaata cgggcggaaa gtaaagtcgc
    17341 accccagtcc atcagcgtga ctaccatccc tgcgttgtta cgcaaagtta acagtcggta
    17401 cggctgacca tcgggtgcca gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat
    17461 ggtttacaaa cgtaaaaagt ctctttaata cctgtttttg cttcatattg ttcagcgaca
    17521 gcttgctgta cggcaggcac cagctcttcc gggatcagcg cgacgataca gccgccaaat
    17581 ccgccgccgg tcatgcgtac gccacctttg tcgccaatca cagctttgac gatttctacc
    17641 agagtgtcaa tttgcggcac ggtgatttcg aaatcatcgc gcatagaggc atgagactcc
    17701 gccatcaact cgcccatacg tttcaggtcg ccttgctcca gcgcgctggc agcttcaacg
    17761 gtgcgggcgt tttcagtcag tatatgacgc acgcgttttg ccacgatcgg gtccagttca
    17821 tgcgcaacag cgttgaactc ttcaatggtg acatcacgca gggctggctg ctggaagaaa
    17881 cgcgcaccgg tttcgcactg ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt
    17941 ttgaagttac tgttgatgat gacgacagcc acacctttgg gcatggaaac tgctttggtc
    18001 cccagtgagc ggcaatcgat cagcaaggca tgatctttct tgccgagcgc ggaaattagc
    18061 tgatccatga tcccgcagtt acagcctaca aactggtttt ctgcttcctg accgttaagc
    18121 gcgatttgtg cgccgtccag cggcagatga taaagctgct gcaatacggt tccgaccgcg
    18181 acttccagtg aagcggaaga acttaacccg gcaccctgcg gcacattgcc gctgatcacc
    18241 atgtccacgc cgccgaagct gttgttacgc agttgcagat gtttcaccac gccacgaacg
    18301 tagttagccc attgatagtt ttcatgtgcg acaatgggcg catcgaggga aaactcgtcg
    18361 agctgatttt cataatcggc tgccatcacg cgaactttac ggtcatcgcg tggtgcacaa
    18421 ctgatcacgg tttgataatc aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg
    18481 tgttcaccaa tcaaattcac gcggccaggc gcctgaatgg tgtgagtggc agggtagcca
    18541 aatgcgttgg caaacagaga ttgtgttttt tctttcagac tcatttctta cactccggat
    18601 tcgcgaaaat ggatatcgct gactgcgcgc aaacgctctg ctgcctgttc tgcggtcagg
    18661 tctcgctggg tctctgccag catttcataa ccaaccataa atttacgtac ggtggcggag
    18721 cgcagcagag gcggataaaa gtgcgcgtgc agctgccagt gttgattctc ttcgccatta
    18781 aatggcgcgc cgtgccagcc catagagtag gggaaggagc actggaagag gttgtcataa
    18841 cgactggtca gctttttcaa cgccagcgcc agatcgctgc gctgggcgtc ggtcaaatcg
    18901 gtgatccgta aaacgtgggc tttgggcagc agtagcgttt cgaacggcca ggcagcccag
    18961 taaggcacga cggctaacca gtgttcggtt tcgacaacgg tacggctacc gtctgccagc
    19021 tcgcgctgaa cataatccac cagcattggt gatttctgtt cggcaaaata ttctttttgc
    19081 aggcggtctt cgcgctcagc ttcgttaggc aggaagctat ttgcccaaat ctgaccgtgc
    19141 ggatgcgggt tagagcagcc catcgccgcg cctttgtttt caaaaacctg cacccatggg
    19201 tacgttttcc ccagttctgc ggtttgctcc tgccaggttt tgacgatttc cgtcaatgct
    19261 gcaacgctga gctctggcag cgttttactg tgatccggtg aaaagcagat cacccggctg
    19321 gtgccgcgcg cgctctggca acgcatcagc ggatcgtgac tttctggcgc atctggcgtg
    19381 tcagacatca aagccgcaaa gtcattagtg aaaacgtaag tcccggtgta atcggggttt
    19441 ttatcgcctg tcacccgcac attacctgcg cagaggaagc aatctggatc gtgcgcaggt
    19501 aacacctgtt tggctggcgt ttcctgcgcc ccctgccagg ggcgcttagc gcggtgcggt
    19561 gaaaccagaa tccattgccc ggtgagcggg ttgtagcggc gatgtggatg atcaacggga
    19621 ttaaattgcg tcatggtcgt tccttaatcg ggatatccct gtggatggcg tgactgccag
    19681 tgccaggtgt cctgcgccat ttcatcgagt gtgcgcgtta cgcgccagtt cagttcacgg
    19741 tcggctttgc tggcgtccgc ccagtaggcc ggaaggtcgc cctcgcgacg cggtgcaaaa
    19801 tgataattaa ccggtttgcc gcaggctttg ctgaaggcat taaccacgtc cagcacgctg
    19861 ttgcctacgc cagcgccgag gttgtagatg tgtacgcctg gcttgttcgc cagtttttcc
    19921 atcgccacga cgtgaccgtc cgccagatcc attacgtgga tgtaatcgcg tacgccagta
    19981 ccatcttcgg tcggataatc gttaccaaaa atcgccagcg agtcgcgacg gcctacagca
    20041 acctgggcga tgtatggcat caggttattc ggaatgcctt gcggatcttc gcccatatcg
    20101 cccgacggat gcgcgccaac cgggttgaag tagcgcagca gggcaatgct ccagtccggc
    20161 tgggcttttt gcagatcggt gaggatctgt tccaccatca gcttgctttt gccgtaaggg
    20221 ctttgcggtg tgccggtcgg gaagctttca acgtatggaa ttttgggctg atcgccataa
    20281 acggtggcgg aggagctaaa aataaagttt ttgacgttag cggcgcgcat ggcgctaatc
    20341 aggcgcagag tgccgttgac attgttgtcg taatattcca gcggtttttg taccgattcg
    20401 cccacggctt tcagcccggc gaagtggatc acggtgtcga tagcgtgatc gtgcaggatc
    20461 tcggtcatca acgcttcgtt acgaatatcg ccttcaacaa acgttggatg tttgccgcct
    20521 aaacgctcga taacaggcag tacgctgcgc ttactgttac agaggttatc aagaatgatg
    20581 acatcatgac cgttttgcag taattgcaca caggtatgac ttccaatgta accgctacca
    20641 ccggtaacca gaactctcat aattcgctcc attaggctta tggtatgaaa taaccatagc
    20701 ataacaaaga tgcgaaaagt gtgacatgga ataaattagt ggaatcgttt acacaagaat
    20761 ttagccgttt tttatgcgcg attaagtgat tataaaacag agggtttatg aatgattgcg
    20821 ctttttatct gaaaaaagac gcggtttcat gcctgcatgc gtcgaaccgt tggccggaaa
    20881 gggcgctaag gccgcctccg gcaaggtcag cactaccgac tcaatatatt tttgtcagca
    20941 catagcgata gagtccaccg tccggcacga actcaagacg atgggtaata caggcaggcg
    21001 catcttcagc gtggtgcgaa acaaacaaca attgcgtttc accttcgcta atcagcacat
    21061 caacaaaacg gcggataagc tggcgattca gcggatcaag cccctgtagt ggttcatcga
    21121 gaataagcaa cgtcggatgt ttcaccagtg cgcggacaat cagcgccaga cgctgctgtc
    21181 cccaggaaag actatggaac ggagcgtcag ccgtgcgttt atcaatgccg agaatatcca
    21241 gccactgctg caccagtttt tgctggcgat ccgaaacggc ctgataaatg ccaatcgaat
    21301 caaaatagcc agaaagaatc acattacgca cggtagtgct gacccggtaa tccagatgca
    21361 aactactgct gacgtaaccg atatgctttt tgatatccca gatggtttcg ccgctgccgc
    21421 gacgtcgtcc gaaaagcgtc aaatcgttgc tgtaaccttg cggatgatcg ccagtaacca
    21481 ggcttaataa cgtcgatttt cctgcaccat ttggcccgac aatttgccag tgttcgcttg
    21541 gattcacctg ccagctaagg ttattaagaa tggggcgatc gttataagaa accacgccat
    21601 tgttcagcac aatgcgcggt tcgttggcgg gtaaggcgtg acgtgctgaa ggttcatccg
    21661 gctccggcag ttgcacacct tcaagctgtt cactatgcgc cagttgcgcg acgagtgctt
    21721 gttggagcag ttcctcttta gcgccagttt ccgctaacgt gcaatccgcc agcacgccag
    21781 caaactggac aaactccggg atctcatcga agcgattgag caccagtacc agagtaatac
    21841 cggactgatg taacgaggcg agtcgctcag ccagctgctg acgtgaggca acatccaggc
    21901 catcgaacgg ctcatcaaga atcaacaagt caggctccga catcagcgcc tgacacagca
    21961 gggtttttcg cgtctcgcca gtggaaaggt atttaaagcg tcggtcgagg agggcggtaa
    22021 taccgaactg ctgcgccagt tgcatgcaac gcggtgcatc ctttacttca tcctgaatga
    22081 tctcagccgt agtgcgtccg gtgtcatctt cgccagggcc gagcatatcg gtgttattcc
    22141 gctgccattc gtcgctgacg agtttttgca attgctcgaa ggagagacga gtgatgtggg
    22201 aaaactggct ttgccgttca cctttcaaaa gcggaagttc ccccgccagc gcgcgggcca
    22261 gggccgattt cccgcttcca ttcgaaccga caaacgccca actatcaccc gcgtttaacg
    22321 ttagctgagg caattgcagc gtttttgtgt cgctaagacg aaacgtgcct tgcaaaattt
    22381 gcaacgatga cattttatat cccattttgt gcagcgatta ctgacaggga tacgtgtttc
    22441 ataacaaatt gtcaacacgc ttagcacagc gtggcgataa tcacgctgtc ggcattaaag
    22501 taggccgtga cattctgtcc ttgctgaaga gaagtcgctt catttaccgg cactgtggcg
    22561 cacagtgttt gcccgtccgg tagcgccatt aatacttcgc actgctctgc gccgcgctca
    22621 atatgactaa taatacccgg taattggttg tcagcgtttt gcgcgaccgc ttcgtcctga
    22681 gtaatgccta cccacggcgc ttttagcaat atcaacactt ctttgccttc atccagcccc
    22741 agacgcgcgc cgctttgtgc ggtaattgcg actttcagac gtgtttttcc gtcagccagt
    22801 aagacatcaa catgctgttg aacgtcatca tgatcgcggg cggtgatggt accgaaccac
    22861 tggttacggg cgctggtttg cagtgaaaaa cgtgagatcg cggccagcag gctgttcagc
    22921 ggcagggcgt catcgtcact taacacatca aaggcttttt gctggatttg cgccagtaag
    22981 tcatagagct gaatcagtcg ctgaccatag cgggtcagta ctgcgccgcc gccaccttta
    23041 ccgcctgttg cgcgctcaac cagaatatgc tcgcttaact gattcatctc gttaatggca
    23101 tcccaggcgc ttttatagct gataccggca tctttcgctc cctggctaat ggaaccggaa
    23161 agcgcaatgt gttttagtag cgaaatgcgg cgcgggtcgg cgaataattt ttgttggagc
    23221 ttaagggtga gaaggatttc ggcctgcata acaatgtcct ggcaaaagtc ttattgtgac
    23281 ggaaaacgaa cgccacgcaa agctgaccgc acaaaagggg agtgcttttc tgtgcttagc
    23341 ggttagaata gtctcatgac tatatctgga gttgaccatg ttagagttat taaaaagtct
    23401 ggtattcgcc gtaatcatgg tacctgtcgt gatggccatc atcctgggtc tgatttacgg
    23461 tcttggtgaa gtattcaaca tcttttctgg tgttggtaaa aaagaccagc ccggacaaaa
    23521 tcattgattc cctgaatgcc cgcttagtcg ggcatttctt tttctcaact tcctgctttt
    23581 ccagccgata ttttttctta tctacctcac aaaggttagc aataactgct gggaaaattc
    23641 tgagctagcc gttatattgt cgcctacata acgttacatt aaggggttac caatggctcg
    23701 taaatggttg aacttgtttg ccggggcggc actctctttc gctgttgctg gcaatacact
    23761 ggcagatgaa gggaaaatca cggtgttcgc cgccgcatca ctgactaacg caatgcagga
    23821 cattgctacg cagtataaaa aagaaaaagg cgtggatgtg gtttcttctt tcgcttcgtc
    23881 atctactctg gcccgtcaga ttgaagcggg agcgcctgcg gatctgttta tctctgccga
    23941 tcagaaatgg atggattatg cggttgataa aaaagcgatc gatacggctt cgcgccagac
    24001 actgctcggc aatagcctgg tcgttgtagc accgaaagcc agcgagcaga aagatttcac
    24061 catcgacagc aaaactaact ggacttcgct gctgaatggc ggtcgcctgg cggttggcga
    24121 tccggaacat gttcccgctg gcatttatgc aaaagaagca ctgcaaaaac tgggcgcatg
    24181 ggatacgctc tccccgaaac tggctccggc ggaagatgtt cgtggcgcgc tggcgttggt
    24241 cgaacgcaat gaagcgccgt tgggcattgt ttacggttct gacgcagttg ccagcaaagg
    24301 ggtaaaagtg gttgccacct tcccggaaga ttcacataaa aaagtggaat atccggttgc
    24361 tgttgtggaa gggcataaca atgcgacagt gaaagcattt tatgattatc tgaagggacc
    24421 gcaggcagcg gaaatcttta aacgttacgg atttacaatc aagtaatgat actgaccgat
    24481 ccagaatggc aggcagtttt attaagcctg aaagtttctt ccctggctgt gctgtttagc
    24541 ctgccgtttg ggatcttttt tgcctggtta ctggtgcgtt gcacgtttcc gggcaaagct
    24601 ctgctcgaca gcgtactgca tctaccgctg gtgttaccgc ccgtagtcgt cggttactta
    24661 ttattagttt cgatgggacg gcgcggattt atcggtgaac gtctgtatga ctggtttggt
    24721 attaccttcg cctttagctg gcgcggcgcg gttctcgctg ccgccgtcat gtcgtttccg
    24781 ctgatggtgc gggcaattcg tctggcgctg gaaggggttg atgtcaaact ggaacaggcc
    24841 gcaagaacac tgggggccgg gcgctggcgc gttttcttta ctatcacgtt accgctgacc
    24901 ttaccgggca ttattgttgg tacggtactg gcttttgctc gttctctcgg tgagtttggt
    24961 gcaaccatca cctttgtgtc gaacattcct ggtgaaacgc gaaccattcc ttctgccatg
    25021 tataccctga tccagacccc cggcggggaa agtggcgcgg cgagactgtg cattatttct
    25081 attgcactgg cgatgatctc cctgttgatt tcagaatggc tggccagaat cagccgtgaa
    25141 cgggcggggc gctaatcatg ctggagctga atttttccca gacgttgggc aaccattgcc
    25201 tgaccattaa tgaaacgctg cccgccaatg gcatcactgc tatctttggc gtctccggtg
    25261 ccggaaaaac ttcgctgatt aacgccatca gtggactgac gcgcccgcaa aaagggcgga
    25321 ttgtcctcaa tgggcgggta ctaaatgatg ccgaaaaaga tatctgcctg acgccggaaa
    25381 agcgtcgcgt tggctatgtt tttcaggatg cgcggctgtt cccgcattac aaagtgcgtg
    25441 gcaatctgcg ctacggcatg tcgaaaagta tggtcgatca gttcgataag ctggtggcgc
    25501 ttttaggcat tgaaccgttg cttgaccgtt taccaggcag cctgtccgga ggcgaaaaac
    25561 agcgcgtggc gattgggcgg gctttgctga cggcaccaga attgctgttg ctggatgaac
    25621 cgctggcgtc actggatatt ccgcgtaaac gcgaactgtt gccttatctg caacgtctga
    25681 cgcgggaaat caacatcccg atgttgtatg tcagccattc gctggatgag atcctccatc
    25741 tggcagacag agtgatggta ctggaaaacg gtcaggtgaa agcctttggc gcgctggaag
    25801 aagtctgggg gagtagcgtg atgaatccgt ggctgccgaa agagcaacaa agtagcattc
    25861 tgaaagtgac ggtacttgag catcatccgc attacgcgat gactgcgctg gcgctgggcg
    25921 atcagcattt gtgggtcaat aagctggacg aaccgctgca agctgcgcta cgtatccgca
    25981 ttcaggcttc cgatgtttct ctggttttac aaccgccgca gcaaaccagc attcgtaacg
    26041 tattgcgggc aaaagttgtt aatagttatg acgacaacgg ccaggtggaa gtggagctgg
    26101 aagtcggcgg taaaacgctg tgggcgcgta tcagcccgtg ggccagggat gaactggcga
    26161 tcaaacctgg cctgtggctg tacgcgcaaa ttaaaagtgt gtcgataacc gcctgattaa
    26221 atcaggtggc tataaatgaa ctgggcaatg ctgtcggtgg tgttatcacc aatcacaatg
    26281 ttggcgcgcg cttttaccgc gtcatcggcg ttgcccatcg ccacgcctgt accagcggct
    26341 tccagcatac tgatatcatt aaagttatcg ccgaatgcca cgacgttttc catcgaccaa
    26401 ccttgcgcct caacccattt cgtcaaacgt ttacctttgc tgttgccgcc gcgtgcaata
    26461 tcaacctgat cgtgccagga ccattcacac tccagtccca gttcatgttc gacatgctta
    26521 ccaaaatgct gcaattgcgg caggtcatcg tgcgtcaggg cgaacttcca tacggcgtta
    26581 acttgttgcg ccgtttcagc cagagaagcg acttgtgtga aagtcggacg ctgttccggc
    26641 ggcagggttt gcgcccagtt agatgtgcga atgacatgcc cggtcgggtg ctcatagacc
    26701 attgcatcat cgacatacat cagaccgtga atgtggtgtt cattcagcat ctcaatgagt
    26761 tgcagggctt taataacggg cattgggtcc gcttccagca cggtttttgc atgataatca
    26821 tacaaatagg tgccattaca gcaaatagca ggtgtatcca gcgccagcgc ctgataaaaa
    26881 ggatgaatag cgacgtgatg gcgacctgtg acgatgatta attgatagcc tgcttcgcga
    26941 gcgcgggcca gggcttctat cgatgaagga agcagggtct ttttcggggt caataaggtg
    27001 ccgtctaagt cgagagcaat cacgcgtgtg gtcatggcgt attccagatt aaggttaaga
    27061 attttcgtct gcgcgaatgg tacaccgata ccactatcgg acaaaattct gcgttttaat
    27121 tcagcattca ccgccaaaag cgactaattt tagctgttac agtcagttgc taaatgcaaa
    27181 ggagcattca tgaagcaaac agtttatatc gccagccctg agagccagca aattcacgtc
    27241 tggaatctga atcatgaagg cgcactgacg ctgacacagg ttgtcgatgt gccggggcag
    27301 gtgcagccga tggtggtcag cccggacaaa cgttatctct atgttggtgt tcgccctgag
    27361 tttcgcgtcc tggcgtatcg tatcgccccg gacgatggcg cactgacctt tgccgcagag
    27421 tctgcgctgc cgggtagtcc gacgcatatt tccaccgatc accaggggca gtttgtcttt
    27481 gtaggttctt acaatgcggg taacgtgagc gtaacgcgtc tggaagatgg cctgccagtg
    27541 ggcgtcgtcg atgtggtcga ggggctggac ggttgccatt ccgccaatat ctcaccggac
    27601 aaccgtacgc tgtgggttcc ggcattaaag caggatcgca tttgcctgtt tacggtcagc
    27661 gatgatggtc atctcgtggc gcaggaccct gcggaagtga ccaccgttga aggggccggc
    27721 ccgcgtcata tggtattcca tccaaacgaa caatatgcgt attgcgtcaa tgagttaaac
    27781 agctcagtgg atgtctggga actgaaagat ccgcacggta atatcgaatg tgtccagacg
    27841 ctggatatga tgccggaaaa cttctccgac acccgttggg cggctgatat tcatatcacc
    27901 ccggatggtc gccatttata cgcctgcgac cgtaccgcca gcctgattac cgttttcagc
    27961 gtttcggaag atggcagcgt gttgagtaaa gaaggcttcc agccaacgga aacccagccg
    28021 cgcggcttca atgttgatca cagcggcaag tatctgattg ccgccgggca aaaatctcac
    28081 cacatctcgg tatacgaaat tgttggcgag caggggctac tgcatgaaaa aggccgctat
    28141 gcggtcgggc agggaccaat gtgggtggtg gttaacgcac actaaccgct gatttacccg
    28201 gcgcagtctc tcctgcgccg gtgtattaac ctatctcctg taacgcgtgt ctctggcgtt
    28261 cgacgatatt ggtccacaaa ttgtctttat cgtcagtcca taaattaatc agcaaggcaa
    28321 aaaagcgttc tgctgctgga gaaagtacgg catctttacg gcgaattatt cccaatgttc
    28381 gacgtataac gggttccacc aatgggatac caataatcga agaatagggc gcatgaggca
    28441 ttgccaggcc aggaagcgcc gaaatcccca gtcccgcctc caccagtcct aatgacgtcg
    28501 aaagatgacg cacttcgtaa aaccagtcca gcttccaggg cttgtcggct agctgttgtt
    28561 ctatcaacag tcggttgccg ctggaggagc gtacgccaat cattttgtaa cccactaact
    28621 cctgccattc tacaagctgc tttttggcca atggatggtc acgccgacag gccagcacga
    28681 acggctcgtt gaccagtggg gtaaaatcaa tggatgaatt tgtgacgttg ttcatgttta
    28741 tgccaaagtc tgattcgtta cacagcaccg attccatgca attattcgtg ccttgttcca
    28801 gaatccgcac tttaatattg ggatacagct cattaaattt accgattgcc agcggtaaaa
    28861 aataaaatac tgcggtcgga atacacgcta acgtcaccat accacgatga taagcattca
    28921 tatcacgaat attaaaaagc gtttcatcga atttctttat taattcccgc gcctccggga
    28981 gcaacctttt tcctgctttt gttaacgtaa ccttgcgagt tgtacgctca aatagttgaa
    29041 tatgtaaatc ttcctccatc ttttttattc ttcgcgttaa tgcaggttgc gtaatattga
    29101 gtaatttagc ggcattgtta aacgaactgg actctgccag tatgacaaat gccttcatac
    29161 ttgataattc atgcttcatt attactccgg aaaatggaag cgacgatttt gggtggctgg
    29221 ccgttaaaaa ttttaactgc atttagccaa cttaaattaa tgaaaaaatg ttattaatcg
    29281 ttgagctaaa gtcattagag atgctttgcc cttaatgtaa ccatatcgca ataagttatg
    29341 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
    29401 ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc agcgcgataa
    29461 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
    29521 taatccactg accagtaaag tcgccattat tagccgttcc agcgatccgc gtgctgatgt
    29581 cgattatctg tttgcgcagg taatcgttca tgagcaacgt gtcgatacca cgcctaactg
    29641 cggcaatatg ctgtctggtg ttggggcatt cgccattgaa aatggtttga ttgcagcgac
    29701 ttcgccagtt actcgcgtac gtatccgcaa cgtcaatacg ggtacgttca tcgaagctga
    29761 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
    29821 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gccggaacca aaaccggaaa
    29881 agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
    29941 tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag gttatgaatt
    30001 accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta tccgtctaca
    30061 agcgggtaaa gcaatgggct taggtgatgt cagtaatatg gttatcccta aacctgtgct
    30121 tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc cgcattcttg
    30181 ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg cattggaagg
    30241 caccgtcacc cgacaaatcg tcccttctgt aggatacggc aatatcaata ttgaacaccc
    30301 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
    30361 atctgttatt cggacgacca gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaaa
    30421 ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata cagaaagaac
    30481 aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag cgatcccatg
    30541 tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
    30601 tatttacctg gcggccattg tggggctggt tatcaagcct ttcccggaac ctgtcgtact
    30661 gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg gtgcgtttaa
    30721 aaccaccgcc gtattaagcg gttactcttc aggtaccacc tggctggtgt tctcggcgtt
    30781 taccttaagc gccgcatttg tgaccaccgg tttaggtaaa cgtattgcct atctgctgat
    30841 tggtaaaatc ggtaacacca cgctgggtct gggttacgtt acggtattcc tcgatctggt
    30901 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt taccgatcat
    30961 caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc gtgtcggaca
    31021 ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt tctttaccgc
    31081 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
    31141 ctggggtgga tgggcgctgg cagccggatt gccgggcatc attatgctgc tggtcacccc
    31201 gctggtgatt tacaccatgt atccaccaga aattaagaag gtggataaca aaaccatcgc
    31261 taaagcgggc cttgccgaac taggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
    31321 ctttgtgctg gcgctgctgg gctggatttt cagtaagtct ctgggggttg atgaatccac
    31381 cgtggcaatc gttgttatgg caaccatgct gctgctgggt atcgttacct gggaagacgt
    31441 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
    31501 ctccttatta tcgaaagtta aattcttcga atggttagct gaagtcttta aaaataacct
    31561 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcattatcgt
    31621 gcgttatttc ttcgcttccg gtagtgccta tatcgttgct atgttaccgg tatttgccat
    31681 gctggcgaac gtctccggcg caccgttaat gttaaccgcg ctggcactgt tgttctccaa
    31741 ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca tctttggcgt
    31801 gggttataac gatattaaat cctggtggtt ggtcggtgcg gtactgacga tattaacctt
    31861 cctggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct ggaacatgct
    31921 gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ttgcgttcgc tcaccccagt
    31981 cacttactta tgtaagctcc tggggattca ctcacttgtc gccttcctgc aactcgaatt
    32041 atttagagta tatccattta ttatctttct gcgcacttca cggtgcgcag atatctggag
    32101 catttgatga tcaagttatc tgaaaaaggc gtgtttctcg ccagtaataa cgaaataatt
    32161 gccgaagaac atttcaccgg cgaaattaaa aaagaagaag ccaaaaaagg cactattgcc
    32221 tggtctattc tctcttctca taatacgtcc ggaaatatgg ataaacttaa aattaagttt
    32281 gattcattag cctctcacga tattaccttt gttggtattg tacagaccgc taaagcgtcc
    32341 ggaatggaac gtttcccgct gccgtatgtg ctgaccaact gccataactc actctgcgcc
    32401 gtcggcggca ccattaacgg tgatgaccat gtttttggtt tatcggcagc tcagcgttat
    32461 ggcggtattt ttgtgcctcc gcatattgcg gtcatccatc aatatatgcg tgagatgatg
    32521 gcaggcggtg gcaaaatgat cctcgggtca gacagccaca cccgttacgg tgcattaggg
    32581 acaatggcag tcggtgaggg cggcggtgag ttggtaaaac agctgcttaa tgacacctgg
    32641 gatatcgact atccgggagt tgttgcggtg catctgaccg gaaaaccagc gccgtatgtg
    32701 gggccgcagg atgtggcgct ggctatcatc ggtgccgtgt tcaaaaacgg ctacgtcaaa
    32761 aacaaagtga tggaattcgt aggtcccggt gttgctgcgc tctctaccga tttccgtaac
    32821 agcgttgacg ttatgaccac tgaaacgacc tgtttaagtt ctgtctggca aaccgatgaa
    32881 gaagtccata actggctggc gctgcacggt cgcggccagg attactgcca gcttaaccct
    32941 caaccgatgg cgtactacga tggctgcatc agcgttgatt taagcgccat caaaccaatg
    33001 attgcgctgc cgttccaccc gagcaacgtg tatgaaatcg acacactgaa ccagaacctg
    33061 accgacattc tgcgtgagat tgaaattgag tccgaacgcg tggcgcacgg taaagccaaa
    33121 ctctcgctgc tggataaagt ggaaaatggt cgcctgaaag tgcagcaggg gattatcgcg
    33181 ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg cgaatgcact gcgcggtcaa
    33241 tcctgtggca atgacacctt ctcgctggca gtttacccgt catcacagcc ggtgtttatg
    33301 gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg caggcgcaat catcagaacc
    33361 gcgttctgcg gcccatgctt tggcgcgggc gatacgccaa tcaacaacgg attaagtatt
    33421 cgccacacca cgcgcaactt cccgaaccgc gaaggctcta agccagctaa tgggcagatg
    33481 tcagcggtgg cgttgatgga cgctcgttct atcgctgcga ctgcggcaaa cggtggctat
    33541 ttaacctctg ccagcgaact tgattgctgg gacaacgtgc cggagtacgc cttcgatgta
    33601 acgccgtata aaaaccgtgt ttatcagggc tttgtgaaag gggcaactca gcaaccgctg
    33661 atttacggac cgaacattaa agactggccg gaattgggtg cgctgactga caatatcgtc
    33721 ctgaaagtgt gctcgaagat cctcgacgaa gtgaccacca ccgacgaact gattccttcc
    33781 ggtgaaacct cttcttatcg ttcaaatccg attggtctgg cggagtttac cctgtcacgc
    33841 cgcgatcccg gttatgttgg cagaagtaaa gcgactgctg agctggaaaa tcagcgtctg
    33901 gcggggaatg tcagcgagct gacagaggtg tttgcgcgca ttaagcagat tgctggtcag
    33961 gagcatattg atccgctgca aactgaaatt ggcagcatgg tatatgcggt gaaaccaggc
    34021 gatggttctg cgcgtgaaca ggcggcgagc tgccagcgtg tgattggcgg tctggcgaat
    34081 attgccgaag agtacgcgac taaacgctac cgttctaacg tcatcaactg ggggatgtta
    34141 ccgctgcaga tggcggaagt gccaaccttt gaagtggggg attacattta catccctggc
    34201 attaaagcgg cgctggataa tccgggtacg acgtttaaag gttatgtgat ccatgaagat
    34261 gcgccggtaa cggaaattac gctctatatg gaaagtctga ctgctgaaga gcgcgagatt
    34321 atcaaggcgg gtagtttgat taacttcaat aaaaaccgtc agatgtaaaa agcgccatgt
    34381 gaatgtaggt cgcattcggc acttattgtc ggatgcgatg cttgcgcatc ttatccgacc
    34441 tacgaatcgc atcgaatctg taggccagat aaggcatttt cgcagcatcc ggcacttatt
    34501 gtcggatgcg atgcttgcgc atcttatccg acctacaaat cgcatcgaac cgtaggccgg
    34561 ataaggcgtt tacgccgcat ccggcaaata gttaattgct cttacttctt cgcctctgca
    34621 accactttac tacccacgcc gcggttattg tattcccaca tgcggttgta gttagtgtca
    34681 ttcagattgc gctgtatttc gtcgttatca tctacgctgc cggtattacc cgcaaacgga
    34741 cgattagaga tcaccgcatc ggcccacggt ttagccgtgt taaaaccttc gttgatggcg
    34801 ctatcacgga tcaccacctg accgttggta ttggcatcaa catccagcga gcggcccagt
    34861 tgcgccacac catcaccgaa agcattgaaa cggctgttta cggcgaggaa accgtagtaa
    34921 atgttggaca gcgtagccgg tgcaaacaca tacgcttctt gctgagtacg tgagttcacc
    34981 acgcggaatt cggtgttatc gaacaccact gcgccgcgac cagaaacgat atccacatcc
    35041 ccttcaatgt agctgttggt caccagcgta cgcggctgac gattcgtttc cagacggttc
    35101 tgcacaccgc tgttggtgac aaagaaggtg ttctgacgac cgagaatgtt aacgttgtta
    35161 atctgtacct ggtcaccatc agtacgcagt gccaccgccg gatggttacc tgcatctacg
    35221 ctatcgccca gcgtgttttc gatggtcaga ttttgcagtt gcaggccatt gttttgtgac
    35281 cagaagaccg cagagcagag aacaccgata ctgtcgctgc gtttgctctg gcagctatcg
    35341 tacatatacc acgctggttt acctggcata tatttgccgc gcgggttgac gtcgtgacgc
    35401 cagtcggcag ggctcatgcc accatcaagg gaaagcccaa tcttcacatc aatcggtttt
    35461 tcacctgtac cgtacagagt aattccaccc ggagcggcag ggacatatac cgttccctga
    35521 tactcaccag gcatcacggc aatatactgg cgcttgttgg tacgcttgat aattgccgca
    35581 tctaccgccg cctgaatcgt ggtatgcgtt acaccttgag tgcccgccgg gccgacaaca
    35641 aagtcaggtt gcgcaggcag ggtaatcggg gaaggattcc acgctgcagc acctggtgtc
    35701 agggatgcaa aatagtgttg agcatcgaaa ttctgcgctt cttttgccga cagaatcggg
    35761 cgagaagagg taccaggcgc ggtttgatca gaaggacgtt gatcgggcgg ggttgagcta
    35821 caggcggtca gcgtcacgcc aaaagccaat gccagcgcca gacgggaaac tgaaaatgtg
    35881 ttcacaggtt gctccgggct atgaaataga aaaatgaatc cgttgaagcc tgctttttta
    35941 tactaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact
    36001 atcagtcaaa ataaaatcat tatttgattt caattttgtc ccactccctg cctctgtcat
    36061 cacgatactg tgatgccatg gtgtccgact tatgcccgag aagatgttga gcaaacttat
    36121 cgcttatctg cttctcatag agtcttgcag acaaactgcg caactcgtga aaggtaggcg
    36181 gatccccttc gaaggaaaga cctgatgctt ttcgtgcgcg cataaaatac cttgatactg
    36241 tgccggatga aagcggttca cgacgagtag atgcaattat ggtttctccg ccaagaatct
    36301 ctttgcattt atcaagtgtt tccttcattg atattccgag agcatcaaca tgcaatgctg
    36361 ttgggatggc aatttttacg cctgttttgc tttgctcgac ataaagatat ccatctacga
    36421 tatcagacca cttcatttcg cataaatcac caactcgttg cccggtaaca acagccagtt
    36481 ccattgcaag tctgagccaa catggtgatg attctgctgc ttgataaatt ttcaggtatt
    36541 cgtcagccgt aagtcttgat ctccttacct ctgattttgc tgcgcgagtg gcagcgacag
    36601 ggtttgttgt tatatggcct tcagctattg cctctcggaa tgcatcgctc agtgttgatc
    36661 tgattaactt ggctgacgcc gccttgccct cgtctatgta tccattgagc attgccgcaa
    36721 tttcttttgt ggtgatgtct tcaagtggag catcaggcag acccctcctt attgctttaa
    36781 ttttgctcat gtaatttatg agtgtcttct gcttgattcc tctgctggcc aggatttttt
    36841 cgtagcgatc aagccatgaa tgtaatgtaa cggaattatc actgttgatt ctcgctgtca
    36901 gaggcttgtg tttgtgtcct gaaaataact caatgttggc ctgtatggct tcagtgattg
    36961 cgattcgcct gtctctgcct aatccaaact ctttacccgt ccttgggtcc ctgtagcagt
    37021 aatatccatt gtttcttata taaaggttag ggggtaaatc ccggcgctca tgacttcgcc
    37081 ttcttcccat ttctgatcct cttcaaaagg ctacctgtta ctggtcgatt taagtcaacc
    37141 tttaccgctg attcgtggaa cagatactct cttccatcct taaccggagg agggaatatc
    37201 ctgcactcgc gtacccatcg acgaactgtt tcaaggcttc ttgggcgtcg ctggcgtgcg
    37261 ttccactcct gaagtgtcaa gtacatcgca aagtctccgc aattacacgc aagaaaaaac
    37321 cgccatcagg cggcttggtg ttctttcagt tcttcaattc gaatattggt tacgtctgca
    37381 tgtgctatct gcgcccacag catccagtgg tcatagcagt cgctgatgtt ctcggcttcg
    37441 ataactctgt tgaatggttc tccattccat tcacctgtga ctcggaagtg catttatcat
    37501 ctccataaaa caaaacccgc cgtagcgagt tcagataaaa gaaatcctcg tcagtgcgag
    37561 gatgctgttc attgctgcta tacacttttt tgctctcaac gtaagcggta gcccattctg
    37621 ttgggttggt gcagttgctt ttaggaaatg ctatttaccc cttaaatttc ggctgaaaga
    37681 gctaaaatcc atgcaaaaaa tttacgcaat tttgtgtatt attgtgcagt aagtaatgag
    37741 ctattttctg cgcaaaaaat ggatggtaaa tttgtccggg tcaggaaaaa ttttatgggc
    37801 gctaaacatg aaaaaagatt cgtatcctta tttgatttgc atgacagttt cagggctgat
    37861 ctttattttc cttttcttct ggtggcgggc agatatctac agggtcacgt ttcttaatca
    37921 gagtatatcc cactattaca ttctgtttag catgggaata gcttttctgt tatctctgtt
    37981 ttgggttaag aaggggatag taaaacaaag aggctggaag agtctgtcag cataccttaa
    38041 ggtttatgca gggatgtgca tatttgctgg attttttctg attatacccc ttacgacact
    38101 aacttatttt ttgcctggag agacatcgtc ttatgttgca ccgtatcggt atacttccgg
    38161 tagttcaaaa agttgttctg gagctgaggt ggatgacccc gaactacatg agaatattcg
    38221 catttgctat ccgtatggca attatgagta cgataatatt atctatgttg aaaagaaaat
    38281 taatatatta ggtgcggtag tgacatatgc acagaccgcg cgtgatgata ctgaatgata
    38341 tagtatatag cgggcaagtt ttagttaatt tatcgaggta atataattta cctcgactcg
    38401 tttgttctgg tattaatatt tcgctttacg accgattttt atctgatgat atcatgcggt
    38461 tttcatatac tgacttactg tcttttctcc gttagcgatt ttctcctgct cagcgatgat
    38521 tttatctttg gcttcta
//
LOCUS       DAZFZC010000038        38533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFZC010000038 DAZFZC010000000
VERSION     DAZFZC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.038"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..327)
                     /locus_tag="ACHKXP_002280"
     CDS             complement(31..327)
                     /locus_tag="ACHKXP_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647270.1"
                     /translation="MLPPLFLLCSVAANAMDYKPVIQSLMNDVCSTSQNVSVCMYQFS
                     AAVKAGKAIGENVELCKKVANEERAMLDCESSESSAQFVDALFDTNRKAVESVQ"
     gene            complement(343..867)
                     /locus_tag="ACHKXP_002281"
     CDS             complement(343..867)
                     /locus_tag="ACHKXP_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7647271.1"
                     /translation="MTTKDITYGIQAELWPRDYTNVEKLLMFWRREQIPVRVTLEDGQ
                     AFCMYVYGLMPSRNKVDLCPAPFDKENRIRLPLERISTIESGVVDSIAHDFKGRLTVH
                     PDYVDNRPSRRDFFAICNQAYKANKSIRVYMADGREIEGVSAGADACQVTLRVENGRK
                     IVVLFDWVERILPF"
     gene            complement(872..1336)
                     /locus_tag="ACHKXP_002282"
     CDS             complement(872..1336)
                     /locus_tag="ACHKXP_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647272.1"
                     /translation="MKSPILFTFCSKTNMKYSLLPLLTLPVLMLTACQSRPISIHDAK
                     PAPQAKVFKYQSAAPATLVVMRDTGFVGAGCDASIFINGETVAKLATGEKATFHLNAG
                     ELIVGASLEGAGLCALNPARQERETTLKNGDTKAFRVFTSNSGDIDILPTTL"
     gene            complement(1363..1641)
                     /locus_tag="ACHKXP_002283"
     CDS             complement(1363..1641)
                     /locus_tag="ACHKXP_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647273.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQVKLKEKNIKMSKAEVINTI
                     LEQLTMADFDKVISSVGASAKTREKIMRIYENSNMTKEDLETLLSRLK"
     gene            complement(1644..3203)
                     /locus_tag="ACHKXP_002284"
     CDS             complement(1644..3203)
                     /locus_tag="ACHKXP_002284"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO7647274.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVETGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQTTRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            3286..3969
                     /locus_tag="ACHKXP_002285"
     CDS             3286..3969
                     /locus_tag="ACHKXP_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7647275.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYYKERM"
     gene            3969..4637
                     /locus_tag="ACHKXP_002286"
     CDS             3969..4637
                     /locus_tag="ACHKXP_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFO7647276.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAV"
     gene            4634..5299
                     /locus_tag="ACHKXP_002287"
     CDS             4634..5299
                     /locus_tag="ACHKXP_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7647277.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHL"
     gene            5296..6186
                     /locus_tag="ACHKXP_002288"
     CDS             5296..6186
                     /locus_tag="ACHKXP_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647278.1"
                     /translation="MTDIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            6196..6462
                     /locus_tag="ACHKXP_002289"
     CDS             6196..6462
                     /locus_tag="ACHKXP_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647279.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            6658..7290
                     /locus_tag="ACHKXP_002290"
     CDS             6658..7290
                     /locus_tag="ACHKXP_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7647280.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKVRAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            7290..8546
                     /locus_tag="ACHKXP_002291"
     CDS             7290..8546
                     /locus_tag="ACHKXP_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFO7647281.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQITIFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMTASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            8573..10147
                     /locus_tag="ACHKXP_002292"
     CDS             8573..10147
                     /locus_tag="ACHKXP_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7647282.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGVAQESG
                     QDQPRGNGDSSTQENE"
     gene            10169..11056
                     /locus_tag="ACHKXP_002293"
     CDS             10169..11056
                     /locus_tag="ACHKXP_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFO7647283.1"
                     /translation="MKLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTHEELLVELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLASGLGKIGLK"
     gene            11082..11957
                     /locus_tag="ACHKXP_002294"
     CDS             11082..11957
                     /locus_tag="ACHKXP_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7647284.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKITSKAY
                     LYNREKTLSEATFIDVNDTIPEGAATFEECVAKLRILAGDVDVDKFLATTMADTNNQL
                     AIQVRQKVKGLARAFRRNLILGDSSSNNKAFDGIPRLMHADQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGVPFIIND
                     FIPTDAGKASIYCLHLSEENGVTGLYGGDNAGIVVENIGTVQNKDAVRTRVKWYCSLA
                     NKHDKAIAALTNVKI"
     gene            12031..12951
                     /locus_tag="ACHKXP_002295"
     CDS             12031..12951
                     /locus_tag="ACHKXP_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFO7647285.1"
                     /translation="MPEQKMKITEEAFSDFTGHMCRAGFTNSISDEPLTERQQSQLSA
                     CLQAVPLSQSVNITPASPSVLVGKTVQLSAGISMGKSASSFTWKSANDQIAAVNGTGL
                     VTGVAPGKVKITATDQETQLSASVEVTVNPVAVQSVTVTPDSTSVEKGKSVSLKANVQ
                     PSNATNKAVTWSSKNEDKATVDQSGNVTGVEVGTATIEIVSQDGSKKATATVEVTAPV
                     VAVTGVEIDPNSTTVEANKTVQLTANVEPAGATNKTVTWESQNTEFATVDSETGVVTG
                     VAAGTATIEVTTQDGSHKATATVEVTAAQE"
     gene            12996..13430
                     /locus_tag="ACHKXP_002296"
     CDS             12996..13430
                     /locus_tag="ACHKXP_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647286.1"
                     /translation="MKPAKIVLLEPQFSGYSGMLCGVQFENGVSVAELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYSDRGELTADLITEPAAPDIVPMKRGTPDEPAKQIQTFTRE
                     ELESIADNEGIAGLRVIGNQVGVKAKGIVEMIDGILKAQGGE"
     gene            13430..14263
                     /locus_tag="ACHKXP_002297"
     CDS             13430..14263
                     /locus_tag="ACHKXP_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647287.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITNGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNTFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDVAAAREDGILSESIGETTNMYRTGKAANVHVARSTWRLLVSYINNRMIVRR
                     A"
     gene            14343..14687
                     /locus_tag="ACHKXP_002298"
     CDS             14343..14687
                     /locus_tag="ACHKXP_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647288.1"
                     /translation="MNISWQTEIAIYRFGAKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLEAAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            14678..15151
                     /locus_tag="ACHKXP_002299"
     CDS             14678..15151
                     /locus_tag="ACHKXP_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647289.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIAGVKPRVGPHFLERAVEISEKQFSEAILEAARKAGFTRG"
     gene            15153..15536
                     /locus_tag="ACHKXP_002300"
     CDS             15153..15536
                     /locus_tag="ACHKXP_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFO7647290.1"
                     /translation="MFIEAFASLMQKAKIGTVGTDIFCHYLPANVKSGVLLINPNTGI
                     SIDHELQGFYLESFTIIVRGSSITTTVEKANKIIEMFPVEETESGGVYFRLVRPMAMP
                     IIYPKNDGALIEAGIPVEFAGYLLN"
     gene            15611..16357
                     /locus_tag="ACHKXP_002301"
     CDS             15611..16357
                     /locus_tag="ACHKXP_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFO7647291.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QLGQTVISELVQGRNITITAPLAESVLQNMVDLMPGSTLSEDDNSVTITSAQGVNLID
                     VAKELVLTPQDTTDYVLTIPKAATAGNFTMTYQSDDVRVFSVQFSAYPDDEGVLGRMS
                     GPKPVKTVSIAPESPTVKAGETVQLTAEITPADAADKTGVWESEDQEKATVDQTGLVR
                     GVAQGSTNISFTSNSGGKKATKSVTINPAD"
     gene            16413..16730
                     /locus_tag="ACHKXP_002302"
     CDS             16413..16730
                     /locus_tag="ACHKXP_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647292.1"
                     /translation="MTKLLDLDSILPPKKRIKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LMNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            16856..17080
                     /locus_tag="ACHKXP_002303"
     CDS             16856..17080
                     /locus_tag="ACHKXP_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647293.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARASQADGEAVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDKADPDAKEQLMQIFGRG"
     gene            17088..21641
                     /locus_tag="ACHKXP_002304"
     CDS             17088..21641
                     /locus_tag="ACHKXP_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO7647294.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASVSVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLISKPVRVRTSGSGDGGSGRRS
                     GASGHSGKSNEGGMFSGLRGNIFLLGEIGDAARTVTDIMFGWQKPIVEAAAEMERMRV
                     MLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLKA
                     LVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGIT
                     MGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFADR
                     VGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQELV
                     INLGKVVAAMVAFKLMRAGIMGVVGAGSQMVSTFATMATAIQTPFNLGATAVTRFNRA
                     ARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMTM
                     LRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKNGEGVNYVSNSA
                     GVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAENQ
                     IFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSLS
                     SRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFERD
                     NAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFEG
                     KPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRSA
                     NASQRAAKKEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLTD
                     LANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPEADSIISA
                     GYTPSREDKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIKE
                     TGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDITN
                     MVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGEA
                     TKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSMQ
                     NLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSLTSSSAKTA
                     TSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAYV
                     PLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMKA
                     IALETIAQEKRPGGSLNPNTQRN"
     gene            21684..22019
                     /locus_tag="ACHKXP_002305"
     CDS             21684..22019
                     /locus_tag="ACHKXP_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7647295.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            22102..22800
                     /locus_tag="ACHKXP_002306"
     CDS             22102..22800
                     /locus_tag="ACHKXP_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7647296.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            22793..23590
                     /locus_tag="ACHKXP_002307"
     CDS             22793..23590
                     /locus_tag="ACHKXP_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7647297.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            23578..24168
                     /locus_tag="ACHKXP_002308"
     CDS             23578..24168
                     /locus_tag="ACHKXP_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7647298.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAVLMVASIWVPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFDG
                     PQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            24186..28811
                     /locus_tag="ACHKXP_002309"
     CDS             24186..28811
                     /locus_tag="ACHKXP_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7647299.1"
                     /translation="MEQFKKKKLPLLIAGAGGKKSKSSSRTPVEADDTVNSRAMVAIL
                     DLLGEGVIGGLVNGAKSIFIDDLPILNEDGSSNFSGITWDFRDGSQDQTPMAGFDFVE
                     TPKSVNIQLKKTHDVTVSIDNDEADRVRVIMKFPSLRSVDKSSGDTNGTTVKYKFQIA
                     NADATFSDVVAEGEKSVDITLTAKKTGVYYRSYELKLPKPGRAYKVRVIRLTDDNSSQ
                     YLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGSTMPSRSYLVRGIKIRVPSNY
                     NEYTNTYVGVWDGTFKLLSSSNPAWILYDLLTNTRYGLGQFVSESMIDLGQLYQIGRY
                     CDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSPS
                     DPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRYG
                     ELKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNKY
                     RAGKRNSGRIVEFAKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKVT
                     FSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETPGSFDITAVENNPSKYEAI
                     DNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISWR
                     RSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGTM
                     TPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTFT
                     HAGLGDSETWYYWVRASNKRGMLSPPNSNLGTEATTRDVLSFLAGKITSSELGQALLE
                     DINSKASQEAVDELNEHINQSVESLEGAVNDVKEDIAELDKQFSDNLADFEIKFNERS
                     DALENAQTELKGEVSATIDKVNEAFEKIDATDAAIVEIENTVSEHDKALANTVEAIKA
                     ARDEAAALIAKESEARVEGDAANAKQLEVLQSTVEESSAAVEEMKKTVAEVDRASAEL
                     TTNIEALAKTNIDLALRQDEDQHKQMVNNAKIATTQKTFADDMSAMATKVEEIRVEIG
                     EDIKASILEESTARADGDEALAKRVTQLQSKFEGDISSAISTEQEARTSADEALTTQI
                     TQLESKVSNDIVAAIKEEQEARATEDSALASQITQLQAKVDGDISAAITQEQEARASA
                     DQALSREINSLRAQTGTDIAAAVAVETKARTDADSALSTQITSLTAKANDLEASLARE
                     TTARADGDTALTKEVSSLKAQTAKDISAAVAVETQARTDADSALSSQITKLTSQYKED
                     IKAAVATETKTRTEQDAALATQITNLESQTAANISAAVTTETTARTQADNALSGRIDT
                     LKAEVDGNTATIQQQATAIADTNKKVSTAWTLKMETSTSGGQKYVAGIALGIDTTGLS
                     QFLVQADRFGLVNSVNGKITTPFVIENSIAYMNGAYIKDGTITNAKVGDLQSTNFVSG
                     RSGWRFGKNGTLEINGNSGGNGRLVINGQRIDVYDDNNVLRVRIGLL"
     gene            29348..33061
                     /locus_tag="ACHKXP_002310"
     CDS             29348..33061
                     /locus_tag="ACHKXP_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFO7647300.1"
                     /translation="MDAAGDKAREAAASAAAAAESANIAGEKASQASQSASAAESSKS
                     AAATSAGAAKTSETNAAASQKSAATSASTATTKASEAATSARDAAASKEAAKSSETSA
                     ASSASNAASSATAAGNSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAAST
                     SAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASE
                     TRAESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATA
                     ASQSKTAAESAATRAEAAADRAEEIAGAVAMEDASLTTKGVVKLSSAVDSTSESLAAT
                     PKAVKVVNDNANSRVPSNRKINGKALTADITLTPKDIGTLNSITMSFSGGAGWFKLAT
                     VTMPQASSIVYIALIGGAGYNVGSPHQAGISELVLRAGNGNPKGITGALWKRTAVGLT
                     NFAWINTSGDTYDIYVEIGNYATRVNIHWDCTANATVSIYTSPTYSASKPSSVTDGVV
                     YTMYSTHQKPTPLDIGALPTTGGTVSGPLSVTGGITGTLNGNASTATKLQTARSIGGV
                     GFDGSANINLPGVNTTGNQNTTGNAATATKLQTARTIGGVSFDGTANINLPGVNTTGN
                     QNTTGNAATATKLQTARTINGVSFDGSANISLSPANIGCPASPTGWLTTGSNGGAITT
                     AQLVTLLQNNGAFNTKSWIARCAWAYANSATIPNSETGCGVIPLAGAVIEVFNNGSSS
                     NNYTIRITTATTTSVSGALTNAEFIYVFNGTDYSPGWRRVYNTKNKPTASDVGALPLT
                     GGTLSGGLTSSGEIISKYANGFRIAYGSFGFFIRNDGSNTYFMLTASGDTLGSWNGLR
                     PITINNTSGAVSIGNGLNVTGGVNGSLNGNASTATKLQTARNINGVKFDGSGDININT
                     LVSRGRVTALSGSTQGTAGIQMYEAYNNSYPTTYGNVLHMKGASAAGEGELLIGWSGT
                     SGAHAPVFIRSRRDTTDAAWSAWAQVYTAKDSIPGVNTTGNQNTTGNAATATKLQTAR
                     KIAGVAFDGSADITLTAANLNAYTKTEVTNLLSSYASRSSLTGYSGNLDIIAETLVVK
                     SGGSGGFAIWDIGTTTSGANMYIDPNPGINTVWRSTSSRRYKKDIETLQDRYADELLS
                     LRPVWYRSICRGDRKDWGYYGLIAEEVGEIAPQYVHWREPTNNDSPEDISSNGMVAEG
                     VMYERLVVPLIHHIQQLTKRVEELETKLNSPKE"
     gene            33122..33376
                     /locus_tag="ACHKXP_002311"
     CDS             33122..33376
                     /locus_tag="ACHKXP_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647301.1"
                     /translation="MSYGAQVWSPSRQEMVDALAPVYYLDYFTPSGSGSRTYEVEAGL
                     GIDYYIMDVTNGKYTSLTVSGNTISWSGASGNLNILVFQK"
     gene            33418..33984
                     /locus_tag="ACHKXP_002312"
     CDS             33418..33984
                     /locus_tag="ACHKXP_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647302.1"
                     /translation="MSPSLSPIVFQRKQVVSLSGGTEFNTQISPDRSPMIFVAYSKAV
                     SLIANRIVRNNQVIYSFGGQGSDSSATIYVFSKGIAKKETWGMSFFNAQGEEIYNTAN
                     IPLSFTFLNNTEWNSSGGHVFDYPPAIIPTYANVFAVPVPGGAMTMVYGYAAYGNTVS
                     SIFVNQLNGGHSFSVNGRVPVINRNLYN"
     gene            34307..34630
                     /locus_tag="ACHKXP_002313"
     CDS             34307..34630
                     /locus_tag="ACHKXP_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647303.1"
                     /translation="MSNEMAGVTTEQVERIAAIVAREVVGKLGKELREEIGQEVNDQL
                     KTYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIVSKITSFIITALLLGLAAYGV
                     KNGLQ"
     gene            34644..35336
                     /locus_tag="ACHKXP_002314"
     CDS             34644..35336
                     /locus_tag="ACHKXP_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647304.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRRHQKGMV"
     gene            35338..35589
                     /locus_tag="ACHKXP_002315"
     CDS             35338..35589
                     /locus_tag="ACHKXP_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647305.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVDVKNETVNEVRQKDASGVHRELLDKWVRD"
     gene            35962..36345
                     /locus_tag="ACHKXP_002316"
     CDS             35962..36345
                     /locus_tag="ACHKXP_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647306.1"
                     /translation="MLNEKRSIQTIRDCTSIIETHVLNQHKLEVRKLNNDAIEPEDSR
                     FIVTFKDDVERAGASVKPLAEAIYLNGRVRFVVKPAKQYPELSRFIEAFSEVIEHSIR
                     RFLRNHGSIVSTVKADDSATKWLLH"
     gene            36330..37082
                     /locus_tag="ACHKXP_002317"
     CDS             36330..37082
                     /locus_tag="ACHKXP_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647307.1"
                     /translation="MAPTLDSAYSKDLSEFPHKGETRVVRFGFLINEASLYKISEIEI
                     IEPEDDICLYVSMERVGARDQGDLSEFILDRADEDAPEEEIIKEVLQSGLLDENKNTI
                     AGRIALREYSFVEDGNEIECYQVAGVETVRERRQRGLCHRTYLFLLHWYEHLVCDDTQ
                     TIPGAKIWAGPLMRTGDVRIYNAKTETFEDVLGEYGMGKETGFLPWNRGLLLDAELSS
                     WLPNKVQVNVQKFIVLIISRKTRTPVGLYLKD"
     gene            37259..37927
                     /locus_tag="ACHKXP_002318"
     CDS             37259..37927
                     /locus_tag="ACHKXP_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7647308.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            37933..38286
                     /locus_tag="ACHKXP_002319"
     CDS             37933..38286
                     /locus_tag="ACHKXP_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647309.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            38338..>38533
                     /locus_tag="ACHKXP_002320"
     CDS             38338..>38533
                     /locus_tag="ACHKXP_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647310.1"
                     /translation="MESIESFLKNFLVSDHRVAVIKGDWGVGKTHYWNSFYTKHSEGL
                     DFNAYSYVSLFGVNSIGDIKK"
BASE COUNT        10732 a         8434 c         9856 g         9511 t
ORIGIN      
        1 cggacttagc cggttattaa ccttatagat ttattgaaca gactctacag ccttacgatt
       61 ggtgtcaaat agcgcatcaa cgaactgtgc cgatgactcg ctagattcgc aatccaacat
      121 tgcccgttct tcatttgcca ctttcttaca cagttccaca ttctcaccta tcgcttttcc
      181 tgcttttacg gctgccgaaa attgatacat gcaaactgat acattctgag acgttgagca
      241 cacgtcattc atcagcgact gaatgacagg cttatagtcc attgcatttg ccgcaactga
      301 gcaaagtaaa aataatggtg gtaacaatat cgatttcata actcaaaaag gcaaaattcg
      361 ttcaacccaa tcgaacaaaa caactatttt tctaccgttc tcgacacgta gtgtaacctg
      421 acaagcatct gcgcctgctg acaccccctc aatttcacgg ccatccgcca tgtacaccct
      481 tatagatttg tttgctttgt aggcttggtt gcaaattgca aaaaaatcgc ggcgtgatgg
      541 ccgattatcg acataatcag ggtgaactgt tagtcgacct ttgaaatcgt gagcgatact
      601 gtcaaccaca cctgattcaa ttgtactaat gcgttcaagt gggagcctta tacgattttc
      661 tttgtcaaaa ggggctgggc aaaggtcaac tttgttacga gatggcatga ggccataaac
      721 gtacatgcaa aacgcctggc catcttcgag agtgaccctt acaggaattt gttctctgcg
      781 ccagaacatc agtagttttt caacgttagt gtaatctcgc ggccagagtt cagcttggat
      841 accgtaggta atatctttgg ttgtcatgtc atcacagagt tgttggcaga atgtcgatgt
      901 caccagaatt gctggtgaac accctgaacg cttttgtgtc tccattcttc agagtcgttt
      961 ctcgctcttg acgagcgggg tttagagcac ataaacctgc accttcaaga gatgcaccca
     1021 caattaattc tccggcattt aaatggaaag tcgctttttc gcctgtagcc agttttgcga
     1081 cagtttcgcc attgatgaaa atggaagcat cacatccagc tcctacaaaa cctgtgtctc
     1141 tcattaccac caacgtagct ggcgctgcgc tttgatactt gaacactttg gcctgcggtg
     1201 ctggtttggc gtcatggatt gatattggac gtgattggca cgcggtaagc atcaacactg
     1261 gtaacgttag tagagggaga agcgagtatt tcatgttagt tttagaacag aaagtgaaca
     1321 gtatgggaga cttcatcttt gtctccctaa ttgatagcgt gattatttta atctgcttaa
     1381 tagcgtttcg agatcttcct ttgtcatatt agagttctca tagatacgca tgattttctc
     1441 acgagtctta gcggaagccc cgacagaaga tataaccttg tcaaagtcgg ccattgtcaa
     1501 ttgttccaga atggtattaa tgacttcagc cttagacatt ttgatatttt tttcttttag
     1561 tttcacttga aactttccaa gtttgtcatt ggccttatca gacaatgcta cctgacagta
     1621 agttgttttc ttttcactca taactaatct cgtttcagaa ctccaaaatc gaatgctcca
     1681 tcaataggca atacaccttc tgcaaagcca ggtgtggtgt cgatgatgtg ttttcgctca
     1741 taagagtgag acaacaggta tttgttgcta atgtcaatga aatcagtgat aaaacacacg
     1801 tttgcctgat tttttttggc tcgtaagcca cgaccgacac gctgtctcat ttcaacttct
     1861 gctttcccac caccagcaag aatgaccgca ccaacgcttg gcacatcaac accaacatcc
     1921 agaatagtcg agcctattaa aacatctatt tcaccagacg ctaaactgtt cagctttgct
     1981 tgccttgtcg tctggttaga ttccccatag atgaaatcaa ctctaagacc tgactccttc
     2041 atcatctcca tcaggatttg cccgtggcgt ttaagacgaa ccagagtcat gcagttgagt
     2101 gaatgttgct tataaagcaa tgcctcgcgc actatggcct cgttacgtcc caaattatac
     2161 acgatcccca actgataagc cttttggtag gcggtgctca taccaactct aaaattgaga
     2221 tgtttgttgg caagttcggc cttgattctg gcctcgtctg gcttgtaggc aactttatga
     2281 taaaggaagt acggctttgc cagaatgcct cgatcaatca ggtacttttc tgtgacttta
     2341 atttcaattc gcccggccac cgccatcagg cgcatgttgg cttccgtcga atccttcatg
     2401 aacggcgtgg ctgtaagcgc cagacgatag tctgcgttca cacataatct ggcgatgtca
     2461 tagaaatttg agcctgaaga ctcatgcgcc tcttccagaa taagaagaga gacacttgaa
     2521 aggaagcgtt tcaccaactc ccgacgtttg aggtggtagc ttttcttatc tggtgttgca
     2581 tcgcgtggtg gttcttcgag gaaacttgca agagtttgaa ctgtagcgac gttgatatgt
     2641 cgtgatacct ggaactcacc cgagccaatg actccaacct tttggtcttt taaccacggt
     2701 tcgccatttt tggcgcggta gtcgatggat ctctggaagt tttcggccat ttgaaacatc
     2761 agaacagagc gggttgttaa aaataatgtc attcgaccga tacgtgcagc tgctttgcag
     2821 gcaacattag atttcccgcc accagtagcg atctgcgcaa tcatcattcc ctctcgaacc
     2881 agtgtttcca cagtctgatc ctgatacgca taatcaggat tgtatgggaa tgggttaacc
     2941 gccggatttg gttttccaag cgccggggct ttgtctttgc ggatatgaac acatttgatg
     3001 cccgccttgt tcaagttcgc cgctacaggc ttggcaaagc ctgcagggaa cgagttttta
     3061 ctccagttga acattgtgct tgtgcccttc cagtcgccag tctcgacttc gtagctcaac
     3121 atctgttgca ccagttgctt taccttgtca tctgcgccag aaataagcgc attaactgcg
     3181 ttagatacaa tccgaacagt cataaacctc tttccttagt gccttttgta tgttatttgg
     3241 ctattataat aagtaagtga ttacttagtg gattgtagca ataaaatgga tgtaaaaatc
     3301 acgattttgc aggttgatgt tgccaacctt cgcccgaata cctggaacac caattcggtt
     3361 ggtgcgcaga attttgaaaa actgaaaggt tctatcgaaa aattgggctt ttttaagcca
     3421 attttggctc gtgaacttga agatggattt tttgaaatcc tcggcggcga acatcgctgg
     3481 cgtgccgcta ttgagcaagg aatttcaacg gttccagtgc tttctgtggg caaaattagc
     3541 gatgtcgtag ctaaacagat gtcactggtg gacaacgagc gatacggtga agacgaccaa
     3601 atcgcattgc aacgcttcat tgaagaaatt cagtcagaac ttgactatca actgtctgaa
     3661 atcgccccgt atgacgacga aatctcgatg gttttagcaa aagaggcggc aatcgacctt
     3721 gaagcactgg aggcgttgtc tcgtggtagt gatgagcctg tcgataccga caaacgagag
     3781 aaaaccgaac gtgtgggggc ggaacatcag accatgcgct tcaaagtaac tttcgatgcg
     3841 tcagatcgtg ttgcagaaac cataaaaaac atcatcaaag agcaggctat taacaccggt
     3901 aatgaaatgg agaacgctgg tgaggctctg gtgtggctgg tcgactacta ctacaaggag
     3961 cgtatgtaat gaccaaaaag tttgaaatcg tatatcgcga cccggcagat cttattccct
     4021 atgagatgaa tgccaaaaag catgatgaac agcagatccg agatctagcc gcagccatta
     4081 aaaagcgcgg atttgaccag ccaattacgg tcgataagaa cgacgtaatt attactggcc
     4141 acggccgccg tgaggctgca atttttgctg gacttgagcg cgtaccggtt attgttcgcg
     4201 atgatctcag tgatgacgag gttcgtgcga agcgccttga agataaccga cttgccagca
     4261 ttgattacga tgcaattaag ctacagaaag agcttgagtc gcttgttctg gacgatatcg
     4321 aggttttcgg ctttgaagag cgtgagttga atgttctcgt tggcagcatg acagaagaaa
     4381 tggacaccga ctcgctagtt atcgatcttg gcgaagaaac taaacgacag aaggatgaac
     4441 acaccgagat cagtcgtgaa gttgcagcgg aagaagtacg tgttgtcgac gtattgggct
     4501 ttaaaacgct tcctgctggc tctgccattg ttgttggtga tttgcttgcc cacatggaag
     4561 aaatgacggg agaaagcggg gtagacgcat ttgtggcata tgcggagaag atctcttccg
     4621 gggagatggc tgtatgagca aatacatcat caacgtatcg tttcagacac gcgtaaataa
     4681 aaccacgcgc acgttggaaa tcgctgagtc gttcgggctt ggcctggacg aaaaagagtg
     4741 gacgctttac gacaatctgg agctggaagt gaagcagggc gatgtggtgt acatcaccgg
     4801 ccaatccggt tccggcaaat ccgttgtgct gcgcgagttg caacgacaga tgaaggatga
     4861 agggctgtct gtagcctcca tcgatgattt taccttcgat aatgaggtta acgtcatcga
     4921 tcagttgggc aaaacgacca gcgatgcgct tgggttgtta tctatggctg gtctgaacga
     4981 tgcatatctg tttgttcgca agccttctga aatgtcagac ggtcagaaat atcgtctcaa
     5041 gattgccaaa ctgattgagt caggcgctaa agtgtgggct gctgacgagt tcggtgctgt
     5101 tctagaccgt gtaaccgctc aggttgtggc atctaacctc cagcgtgccg ctcgaaaggt
     5161 tggtgcgacg gtaatggtgg cgacgactca cgaagacctg aagaacgcgc tgcgcccgga
     5221 tatgcagatc accaagcact acaaagaacg cgtgaaggtg gaatatcaca atggtagtca
     5281 tgatgaggtt catttatgac ggacatcatc attaaacgct accgccctga agagtttccg
     5341 cgtcatctgg actttctgga gcgaatgact gtcacaaagg gaactgtaga ggactggcac
     5401 gctcttaagt cgcttcacta caaaacagac ggcaaacctt tcgcgccaac ttactatcgc
     5461 tgcgaacttg atgaccgtct ggtgggcgtc gtggttatgg cttacccgaa actactgctg
     5521 gcacctcgcc accgcatgtt tcctaagttg aaaccaacca ctaataccac cgtggctaac
     5581 cagtactggg gtcggtacgt gaataacaac tttgcggtga tcagtcgctc agttgtggat
     5641 actcagtatc gtggcgtagg cgtctcttat cgaatgatta acctggttag caggatgcat
     5701 gaccggccaa tcattgagat ccagtcctcg atgagcaaat acaatccctt cgccatgaaa
     5761 gcagggttta agttcatccg ccctgagcga ccgaagagct atgaaagtgc actgcgtgta
     5821 ttccagcgcc atttccgttc cgacccgggt gataacgagg cgatcgtcaa agaattgttc
     5881 gcaatgagcg agtctcgtcg tcgccgtgca ctgcgtgatc tggtggcgga ctaccacaag
     5941 aacagttccc tggcaaaagc tgggcggaat cgtggcacga cgattcagga cattgccgac
     6001 agtctggtgg acgaggccag cattgtgaag ctgctcaagg acattcacaa cctgagcttt
     6061 acgtctccgt tgtatggtgt gtaccgaaac cctgactttg gccgtcgact gcctgacacg
     6121 ctgccactgc tggcattcga caaacaacct ttggataaac cgttagaaat tgctttaccg
     6181 gcataaggat ttgccatgac gttaaccgat aaacaaaagg acatcatcaa aacgctcaat
     6241 ctcggttatg agcgaggtca tctacttgac ctggacgaat tgcttgaagt tttgccgtac
     6301 aagacaacca agcaaagtat ccagttctca attcgcgctc tgataaaaaa ggggctggtg
     6361 gagaaagggc atacgcgcca acgcagtgac aatcgctatc accgccgaac tcttgggtta
     6421 accactttag gtcgagccaa agcgaagtta ctggtgatgt aatcggtctg ggagcttatt
     6481 taaagacctg catctgtata tataaataat aagtaactta ttaaatatat acggaagcag
     6541 gcttcgtaag acatgccaga cctaattaaa caccccagaa aacaagttgg ttagcagatg
     6601 cagtaaacaa gttgttttag agcgcatgga cgcgctctgt gtgttttaga gggatctatg
     6661 acggtcgaaa aagacgaggt aaaaactcgc ctgacaccag cggagtgggc cgaagctgaa
     6721 gccaaatgga cgtcaggcga atatacactc tcaaagctgg aggaagagta cggcattcgt
     6781 cgtgaaacac tctccagaca tttcaaaaag cgaggattag agaaaggcgc ggactctgtt
     6841 gggaagatgg ttcgtgagtc tcttaaatct gacgcagagc ttcgcgctaa agtccgtgcg
     6901 gaaaagatag aagaacgtcg tacacgttat gacggctggg cgtatgcgtt ggggcagatg
     6961 gtgatggtcg aagtcactac ggccaaacgt gagggtaagc ctttaggggc gattgaggat
     7021 tctctcaaga gcttacagag agccagtaat acccttgcaa aatgctttga agtttcgtcc
     7081 aaagcattgg gcatggatca tgcggaaaat gacgaggaag aaattccgaa tctggtattt
     7141 ggtgagctta cgccttccca ggtggcgaaa ttacgtcagg aagacgacga gcctgaaatc
     7201 atcgatgacg aattgcttga gacgttggaa gaagaagctc taagcgaatt tgatgcgaca
     7261 gatgatggaa gtgaagggga ggacgaataa tggcaatccc gtcctcgctc agtcttgtgc
     7321 aactgcattc tggacagatg aaagtcttcc agtctccgca tcgatttaaa gttgtttgtg
     7381 ctggtcgacg ctggggaaaa tcccggttgt cgatctccac tattattcgt gcggcggcaa
     7441 aggaaaaaaa gcaaagggtc tggtatgtcg ctcctactta ccagatggct cgccagattt
     7501 tgtgggacga tctacaggaa gttctgcctc gtaagtgggt taggaaaaag aacgacacca
     7561 cgatgacaat cgtgttgaag aacggttcgg agatcgccct caaaggtgct gataagcctg
     7621 acactctgcg cggcgtagcg ttgcattttg tagtgcttga tgaatttcag gatatgaagg
     7681 ctgacacttg gtacaaggtg ttacgaccta ctctttcatc gacacgcggc ggtgcactga
     7741 tcattggtac gccaaaaggc ttctcggaat ttcacaaact gtggactata ggccagaacg
     7801 tagagctgca aagaaaggga cagtggaaga gctggcagtt tgtaactgcc gattctccgt
     7861 ttgtacctac ggcggaaatt gaagctgcta agaacgatat ggaccccaaa tcgttcgctc
     7921 aggagtacct ggccagcttt gagaacatgt ccgggcgcgt ttactacccg ttcgatcgta
     7981 acgtgcatgt aaaaccgctt cagttcaacc ctcggttacc tatatgggta gggcaggact
     8041 tcaacattga cccgatgtct tccgtaattt tgcaacctca gccaaatggt gagctatggg
     8101 caattgatga attggtgctc ttttcctcta acacggcaga ggtttgtgat gagcttgaga
     8161 gacgcttctg gcgctggaaa tcacagataa cgatatttcc agatccggca ggtgcttatc
     8221 gccaacatgc tcgcggggag tctgacgtcg acatattcaa agagaaggga ttcttacgtg
     8281 tcgattattc gaaaaagcac ccgccaattg cggatcgtgt taatgctgtt aaccgaatgc
     8341 tgatgaccgc atctggagat atccggctgt atatcgatcc gaagtgcaag catttgattg
     8401 attcactgga aaaagtcatc tacaagcctg gaacacgaga tatggataag acaggtggca
     8461 ttgagcatag tgcagacgca ttgggctatc cagtacatcg taggtatcca gtcaaaaacc
     8521 gtgttattct tggtggttct cgataggtaa gtaattatct aaggttattc aaatggaatt
     8581 gaacgacaaa caaattaagg atctggtggc gcgacgccac ccggaatacg aaaagaaaaa
     8641 agaacattgg gacttcctcg ccagcactta cgctggcggg cgtggttggt ttacagacaa
     8701 tatctttcgt tactttaaag agggagatca ggagtttaag gagcgagttg aacgtgctta
     8761 tcgcttcaac cacactcgtg aggtggtaaa cctcatcaac aaatatctct ttaaagaaga
     8821 cattcataga aatatcgaag aggcaccaga gcagatccgc aatttctgga aacgtgcgac
     8881 tcgccagaat gcctctattg actcatttat ggccgctatt gatttgcagt cgtctattta
     8941 cggtcgcata tgggttgttg tcgatagcac gatgagtggt gatgttgagt cagtagctga
     9001 cgagaaaaag aaagatgctc gcgcctacgc ctactggatt tcacctcagc aaatgctgga
     9061 tgtggcatgg gacgacgacg ggaatatgtt gtgggcgtta attgtggaag tcgctcgtga
     9121 tgacgcagat ccttttactt ctacaggtca ggaataccaa cgttatcgtc tgtggacaca
     9181 aaacgagtgg tatctgttcc gtgaggaagt gaagaagggc gctggtggag caggtcgccg
     9241 tcaggcaaaa gttattttag aggatagcgg tgagcataat ctcggcgtag ttcctgtgtt
     9301 tcctgttgat tgtattggag aaagtgaatc accgtatttc agcccatcgt tgatcgatga
     9361 tatcgcttat cttgatcgtg cggttgcaaa ctatctgtca aaccttgatg ccattattca
     9421 ggatcagaca tttagccagt tggctatacc ggtacagtcg cttttacctg gtgatgaaaa
     9481 ccacactaaa gtgcttgaaa tgggcacaaa gcgagtcttc acctacgatt ctgaaggtgg
     9541 aaaccagccg ttttatctgt caccagaccc gaaacaagct cagatgatca tcactacgat
     9601 taagacggtg attaacgaaa tctaccattc agttggtgta gctggtgagc gaacgaagca
     9661 ggacaacgca cagggaatcg ataactcatc tggggccgca aaaatgtatg acttccagcg
     9721 tgtaaatagc ttgcttgtca caaaagcaga gcgtctggaa agggctgaac gccaaatcat
     9781 gctactggtt gcgaaatgga tgggggtaga tctggacgaa gaccactctt taattgcgta
     9841 tccagaaagt ttcgatattc gtggccttac agatgaattt tctgttgccg agaaactgtc
     9901 attacttcag gcaccggact ctgtacgtcg tcaccagatg gaaatgctta ttgagaagat
     9961 tttcccgaac attactgagg cgatgaaaaa ggaatttgat aaagatctct tgaattttcc
    10021 gccaaaaaat gatctaaata cccttgaaaa taagtcagta cttacttatg atcgtggtgt
    10081 ggcccaagaa agcgggcaag atcaaccccg agggaatggg gactcatcta ctcaagagaa
    10141 cgagtgataa gtaacaaagg gaatttttat gaaactgtgg caaatgcttt tggcccgtcg
    10201 tggtctgatg gatgttgctg aagcgcatga gcgtgggggc gctggcggtg tagctgctga
    10261 taatgagcag agtacacaag atcctgacaa gcagggtgaa caaaaagagc agccgaaggg
    10321 tgatgacgaa tacgctggca tgactcatga agagttactg gtcgaacttc gcaaaaccaa
    10381 gaaagctggt gctgaactgc tgaaagagaa catgaagcgc aaagagaaag agcgcacatt
    10441 ggccgatcag cttgctcagt acggtgatat cgacccggcg cgtgctcgcc agcttttaga
    10501 agctgaacag gccgcagaaa acgcacgtcg tgaggcggag caagctgaac tggagcgtcg
    10561 tggtgagttc gatgctgtta aaaaacagat gatcgaagca caccaggcag agctggcaca
    10621 gcgtgacgaa cgttatgcag cactggaaag tgaaaacgca tcactgaaat ctcaattagt
    10681 cgagatgacc gtgggcgctt ccttcagtaa ctctctcttc ctacgtgaca aagttctgat
    10741 gactccggca aaagcccgcg tgatctacgg ttctcatttt gaagtgggtg aagacggtag
    10801 cgtagtgggt tatgacaaac cggcaggtca taaagaacga gctgttctgg ttgacggtga
    10861 aggtaagccg ttgccgtttg aatccgcgat tgaacgcatt gtgcgggcag atccggaagc
    10921 tgacgcattg atgcgtagcg aagccaagca gggtgtaggc tcacattcca aatcgaccta
    10981 cacaatatcc caaccgaaga acaagtcgac tatggataag ttggcctccg gtctggggaa
    11041 aattggactt aagtaacatc taaatcaaag ggaattgata gatgccatta ctgcgtgaag
    11101 aagctgaaaa gctgtctaac aacgaacttg aacagggcgt gattgaaacc atcatcgatc
    11161 gcgatgacct gtttgccatc ctgcctttta tgaaaattac ttcaaaggca tatctgtaca
    11221 accgtgaaaa aacgctgagt gaagctacct tcatcgacgt taacgacacc atcccggaag
    11281 gtgcagcaac cttcgaagaa tgcgttgcga aactgcgtat tctggctggt gacgtagacg
    11341 ttgataaatt cctggcaacc actatggccg acaccaataa ccagttggct attcaggttc
    11401 gtcagaaagt taaaggtctg gctcgtgcgt tccgccgcaa cctgattttg ggtgactcca
    11461 gctccaacaa caaagcgttt gacggcattc cacgcctgat gcacgcagat cagaagatcg
    11521 atatcgccgg tgcatctatg actttctcta tgttcgacga actggtcgat gcggtgaaag
    11581 atcttggtgc tgactgcatc atgatgcgtt cagagcacct gcgtgcttat cgtgcgctgc
    11641 tgcgtacagt aaacgtaggt ccgtctgaaa tcatgatgga gaactttggt cgtccgatgc
    11701 tgtgtcataa cggcgttccg ttcatcatca acgacttcat cccgaccgat gctggcaaag
    11761 caagcatcta ttgcctgcat ctgtcagaag agaacggcgt tactggtctg tatggcggcg
    11821 ataacgcagg tatcgttgtt gaaaacattg gtactgtaca gaacaaagac gcagtacgta
    11881 cccgtgtgaa gtggtactgc tctctggcga ataagcacga taaggctatc gctgcactga
    11941 ccaatgtaaa aatttaatca gtgtggtaga taagcaatta tctatgttta agggtgggct
    12001 atacgcccac cctttttgta ggagcaagaa atgccagaac aaaagatgaa gattacggaa
    12061 gaggcatttt cggattttac ggggcatatg tgccgcgccg gatttaccaa ttctatctcc
    12121 gatgaacctt taaccgagcg gcaacaaagt cagctatctg cttgtttgca ggcggttccc
    12181 ctctcccaat ccgtaaacat aaccccggct tcaccgtcag ttttggttgg gaaaactgtt
    12241 caacttagtg caggtattag tatgggtaag agtgccagtt cattcacttg gaagtcagct
    12301 aatgatcaaa ttgcagccgt taacggcact ggattagtaa ctggtgtagc accaggcaaa
    12361 gtaaaaatta ctgcaactga tcaggaaacc cagctttccg cgtcagtgga agtcaccgta
    12421 aatccggtag ccgttcaatc cgtaacggta acgccagact caacctctgt tgagaaaggg
    12481 aaatcagtca gtctgaaagc gaatgttcag ccgtcaaatg cgacgaataa ggctgttacc
    12541 tggtcttcaa aaaatgaaga taaggcaacg gtagaccaga gcgggaacgt tactggtgta
    12601 gaagttggca ctgcgacgat tgagatcgtt tcccaagatg gcagcaaaaa ggcaactgca
    12661 acggtggaag taactgcgcc tgttgtggct gttaccggcg tcgaaatcga cccaaatagc
    12721 acaaccgttg aggcaaacaa aactgttcag ctgaccgcaa atgtcgagcc ggcaggagca
    12781 acaaataaaa ccgttacttg ggaatcccaa aataccgagt ttgcaacggt tgatagcgaa
    12841 accggtgttg taactggcgt tgcggctggt actgccacaa tcgaggttac tacccaagat
    12901 ggtagccaca aagcgacagc gaccgtagaa gtcactgctg cacaggaata atcaatattg
    12961 ggcggcgaaa gccgcccatg tagagagaaa gcgttatgaa accagcaaaa attgttttat
    13021 tagaaccgca attttccggt tattcgggaa tgctgtgtgg cgttcagttc gagaacgggg
    13081 tatccgtagc ggagttgcct tttatcgatc agcagaggat ttgtgcttca atgcgagcgt
    13141 caacagtaga gggcaaaaat gtttctccgt ctgccgcata cagtgatcgt ggtgaattga
    13201 cagcagacct gattaccgag ccagcagcgc ctgacattgt gccaatgaaa cgtgggacac
    13261 cagatgaacc ggccaaacag atccagactt tcacacgaga agagctggag tcaattgcag
    13321 acaacgaagg tattgccggt ctacgagtta ttggtaatca ggttggtgtc aaagcgaaag
    13381 gaattgtcga aatgattgac ggcatcctga aagcacaagg tggtgagtaa tggcgcagat
    13441 cgactcgtat cgtagcggtg aagctgtttc tctttcattt gccttcaacg tgctggatat
    13501 cgaatctgcc acctacacag taaaggacag caccggggct attctcgttg atggcgagcc
    13561 gctagaaatt accaacggtc aaatgtcgat tccggttgtc gtgtcggctg aatataacca
    13621 gctctctgag aaagagcgag atctgcggta cgtcattgtg aaggctgttg catcgggcct
    13681 gacgcatgaa gagcgtcaaa tgtatgttct gctgaatact tttgaactgt cgataccaga
    13741 acagtcgttt gcaactgtcg ctgatgctca aatgcaggcg atcgatatgc tgaatgggga
    13801 cactttgctg tcggatggtg aaggcttaat gcgcaagcgt ctcattgagg ctaccagacg
    13861 aattaaaact ttaccgttct caatccgcaa aattctacgt atcgactttg accgatacga
    13921 tcgccctcaa aatatgctga atgtgtatga cattccgtgg ggagcagatg gagcgtaccg
    13981 gcatgattta gtcgattggg aaaagatgac gcaggagaag tttgaagagt tccctgacta
    14041 cttcaaagaa gcgttgatgc ttgccgttgt caacgaagcg tgtgagatcg caaatggcaa
    14101 tgatgtagcc gcagcacgag aggatggcat tttgtcagag tccatcggtg aaacaaccaa
    14161 catgtaccgt accggtaaag ctgcaaacgt gcatgtggct cgtagtacct ggcgcttgct
    14221 ggtcagttac atcaacaacc gtatgattgt tcgccgtgcg taacgccagt cgcattattt
    14281 acttttggtc taaagggttt ttggccagta atgagagcga ccgacaacaa cagggagaga
    14341 gcatgaatat ttcatggcaa acagaaattg cgatctaccg ctttggcgca aagaacgttt
    14401 acggtgaggc gcaattacaa ttcgtcagga agacgaatgt cggcgtggtt aagttcgaac
    14461 agagtaatga gaaatcgtca gttcgtgctg atagctctgg tagtcgtggt aaggcgagtc
    14521 tggagctgtt tgatgctgtg ttagttgtcc ctttggaagc ggctgtacaa cttgatgatg
    14581 tactgatcct tgaaggtcaa aaattaaagg tgtccagtgt tcatcgccgc tgggggctac
    14641 gaggaagacc tgggcatctt gaagtagggg cgaatatatg ggtctgaaat acgatgcgca
    14701 tcagttcaaa cgtgcgggga atagactcaa caacagccag aaagcattta agcgatatct
    14761 tattcgggac atggaaaagc tggcgcgttt ggttgagcgt ctcagccgtg caatggcccc
    14821 gctggaaacc ggatctctgg agacagccat ctttgccagg gtgattaaag aaggttattc
    14881 agggctgcgt attgagcttt ctgtgtctgg agcaaaacca cgtcaggggc atccgggcgt
    14941 agaagtaggt gactatgcta agtacatgga gctgggtaaa taccgactcg gttatctttc
    15001 tcgaatgaag aacgtgacaa acccaccaat tgccggtgtt aaaccccgtg ttgggccaca
    15061 ctttctggaa agagcggtgg aaattagcga gaagcagttc tcagaagcga ttcttgaggc
    15121 tgccagaaaa gccggattta cgagaggtta atgtgtttat tgaagcgttt gcaagtttga
    15181 tgcagaaggc aaagattggc acggtcggga ctgacatttt ctgtcactac ttgcctgcca
    15241 atgtgaaatc tggcgtcctg cttattaacc caaataccgg tatcagcatc gatcatgagc
    15301 tacagggctt ttatctcgaa tcattcacaa tcattgtgcg tggttcgtca attactacga
    15361 cggttgagaa agccaataaa atcattgaga tgtttccagt agaggaaacg gaatctggtg
    15421 gtgtttattt tcgacttgta cgaccgatgg cgatgccaat catttatccc aaaaatgatg
    15481 gggcattaat agaagcaggc attcctgttg aatttgctgg ctatttattg aattaactca
    15541 attagtaagt atatacttac tattagcgca atgaatgcgt agaattaacg gaaaaaggag
    15601 ttttccatca atgtctaata cccatgtaaa aaacatcaaa cttggtgcct gtaaggtgtc
    15661 gtttggtggt gtggatttgg gttacaccaa aggtggtgtt caggttgaag tagccactga
    15721 aacgctgaaa gtcaccgtcg atcagttggg gcagaccgta atttccgaat tagttcaggg
    15781 gcgcaacatt accatcaccg cgcctctggc tgagtctgta ctccaaaaca tggttgacct
    15841 gatgcctggt tctactctga gcgaagatga caactctgtc acgatcacat ctgcacaggg
    15901 cgttaacctg attgatgtgg cgaaggagct ggttctgact ccgcaggaca ccaccgacta
    15961 tgtgttgacc atcccgaaag cggcaaccgc gggcaacttc actatgacct accagtctga
    16021 tgatgttcgt gtgttctctg ttcagttcag cgcataccct gatgacgaag gcgttctggg
    16081 aagaatgagc ggccctaaac cggtaaaaac tgtgtcgatc gcaccggaat ctccaaccgt
    16141 aaaagctgga gaaactgtgc aactgacagc cgaaatcact ccagctgatg ccgcagacaa
    16201 aaccggtgtt tgggaatccg aagaccagga aaaggccacg gtagatcaaa cgggtctggt
    16261 acgtggtgtg gcgcagggtt ctacaaatat ctccttcacc agcaatagcg gtggtaagaa
    16321 ggcaactaaa tcagtaacga ttaaccccgc agattaatca gtaattaagc agaggctcag
    16381 gatgggcctc tcttttaaaa ggattttaac caatgaccaa attactcgat ctcgattcca
    16441 ttctgcctcc gaagaaacgc atcaaatttg gcggtcagga atatcccatc gttgaaatga
    16501 cggtaggtct gttcgtttcc atcaagcaga tggaaggtaa agatctcatg aacatgtctc
    16561 ctgttgagca agtgacagct tatgcagatc tggttcgtaa ggttatccct tcagtacctg
    16621 acgaagttct tgaaaaactg actgttccgc aactccagca gatcttcacc ttcgctatgg
    16681 aagtgattga tgaagaaaac gaaaaagcgg ctggcgaagg ggcaaagtaa tttcccgcga
    16741 tgaatccggg acaaggaccg tctcaataga tctcggattc tatttcagtc gtgtagttgc
    16801 tcactacgcc gtgtcgccat tagagctact gaacgttcct ctcacgatgt tctggatgct
    16861 cagtcgcaat atagaccgtc tgcgtgcgga agaggatgtc cgcaacttac aagtcgctcg
    16921 tgcgtcccag gcggatggcg aggctgtgaa ggcgttcatg gagggtttgc aactcaggat
    16981 tggaagacca gtcgtaacag ataaagtcta cgatccaagc caggataagg cagaccctga
    17041 cgccaaagag caactgatgc aaatatttgg cagaggatga caagggaatg tcacaaaacg
    17101 tagagtttat cctgtcgctg gaagacaaac agtttacagc gtcaatcgat cgtgcgggga
    17161 aactgcttac tcgatttggt gagcaggtaa caaagcctgc tcaaaaaatc caaacctttg
    17221 aacgctcttt gggttcggtc gcccgtatca ttggcgttct ggaaagcaag ctcgattcta
    17281 cggcagataa actacaggat gtagctgccg gttttgagct tgcttcagat gctacgcgta
    17341 aaatgcgtgg caacatcacc agcctcaatt ctggtcttaa agccctgatt gagcgcgtcg
    17401 atacgacaac ttcttcagta gataaactca ccgcgtcatt gcgtaaagtg caatcagagc
    17461 taaatgattt ctctgattgg gcgacctatg caagcaaaag cgcaagccgc tttggtacgg
    17521 aggtcaaaga agcctctgtg tccgtgagtg gcatgaatac gcgccttaat accacgacga
    17581 agcgactcag taattggggt gtcacaacga gtcaggctgc cgagggactg aaaaaggttc
    17641 gtgagcagat ggacgaagtt atcggacgtc agcaactgat tagcaagccg gtacgcgttc
    17701 gtacatctgg aagtggcgat ggtggtagcg ggcggcgtag cggcgcttct ggccatagcg
    17761 gtaaaagtaa cgaaggaggt atgttctctg gccttcgtgg caacattttc ctgcttggcg
    17821 agatcggaga tgcagccaga acggttactg acatcatgtt tgggtggcag aagcctattg
    17881 ttgaagctgc ggccgaaatg gaacgtatgc gggtgatgct tcgagggttg aataaggaga
    17941 agtccaaccc gggccaggct gccgctgatg atatgaagta catcgtagac atggctcaaa
    18001 atgcgccgtt cgcgatgcag gcgttaacag actccttcgt gaagtttcgt tctgctggct
    18061 tagatccaac cgatggttct ctgaaggcgc tggtggactc cgttgctcgt tttggtggtg
    18121 atagtgagct gttgaaacgt gcggctgtgg ccgttcagca gatgtccggt aagggcgttg
    18181 tgtcaatgga agagctacgt cagcaattag gtgaagccgt tcctaacgcg atgcaggcaa
    18241 tggcagacgc cgcaggcatc actatggggg aactgactaa agccgttgcc agcggtacgg
    18301 ttgaggcaaa acaggcgttg tctctgatgt ttgttggtct gcgtgcggag aatgagaacg
    18361 cagcgaaaga catgatgcaa acctacacag gtgcgctggc gcaacttcag acgtcattca
    18421 cgttatttgc tgatcgggtt ggtcaggccg gatatctgga ttctctatcg aaggggatga
    18481 aagaactggc ttcaatcatg aatagcgccg aagggatttc gtttgccaat tctttaggtt
    18541 cggggttaac aacggcaatc gatgggttgc gtcagcttgc tcaatggtta gcaaagaacc
    18601 aagagctggt aattaatctc ggtaaggtcg tggccgcgat ggttgcgttc aaactgatgc
    18661 gagcagggat catgggggta gttggcgcag gcagccagat ggttagcacc tttgccacga
    18721 tggcgacagc catacagact ccatttaacc tcggcgctac agcagtaacc cgattcaatc
    18781 gtgcggcacg tatggggctg gctccgatcc cctctcttat tttcgccatc cgtggggcga
    18841 ttacggggct taaaggcgct tttgctggat taacggcgtt cattgcagca aaccctatag
    18901 gggcagcatt tacagttgcc actgtagctg ttgctggcct aatcacgtac atgaccatgc
    18961 tccgcagcga aacttcaaag gtcgttgacg agattaggaa aataccagag gcgatgacag
    19021 cggccaaacg agcacagatg gccgatcgcg cagaaaagct cgaaaagcag attctcgctg
    19081 atcaacgagc attaaaaaat ggtgagggcg ttaattacgt atctaactct gccggtgtta
    19141 ttactcacaa agagtcgaag gctgacatcg aagcacgcct gaaaaaaaat caggaagagt
    19201 atcaaaagat aaccggcacg atagctcttg gtgacggcgc tgtagcaaag cgtttggcta
    19261 aagaggctgc cgaatctcag attgagaaaa ttcgagcgga aaaccagatt ttcgcggcaa
    19321 cattcgtgaa agcccggcag gaggctctgg ataagatcca gaaaatcaat gatgacggtt
    19381 cactttcaga tgacgaaaag aacaagctat tggcaccgtt acgtgaaacg gtaaacaaaa
    19441 gctatctgga gcctgcgcaa aaactggttg attcactttc ttctcgtaag aatgcgaccg
    19501 agaagcaaat cgcgactctt aatgatcagc ttgaaaaagc caaaaaggat ggaaataccg
    19561 agcagatcca gaaactgcaa ggcagtattc gtggttatca ggagcatttg gaagccgttg
    19621 ctcaggagct gactcaggca gagttcgaga gagataacgc ggccaaaact ggtaagggcg
    19681 taatgtcaaa ccaggggact gttcttgggt taggtacaac tgataaagct gctcagaagg
    19741 cgctggcgca atatatgcga aaccagatgg attctgcgac ttatcaacgt actttgcctg
    19801 acggcactcc gatgatggac ttcgaaggta agccgattat tgggcctaaa caactcaaga
    19861 cgcagcttaa tttgcagaaa gcatccagcg ccagctctct ggagaaaatg agcgatgaag
    19921 agcgtgccgc agccattgct gcactgacta aagctcgtga acaggatgca gcagctgccg
    19981 agaaagcagc ccaacgatca gctaacgcct cgcagcgtgc ggccaagaag gaacaggcag
    20041 cgcaacagaa actggcagcc ggataccaga aggctctgga caaagccgat cagcttatgg
    20101 ggcaaatggg tgaaagctct aaggctacgg tatcgtttga tcagtctctt cgcgatacaa
    20161 cgaaatcgct gacagatttg gctaatgcgg ttcctaacga gttcatcact caagagatga
    20221 tcgacaaggc gaaaaaacgt cttgaagacc tcaaaaatgc gacacctgaa tatcgcgaga
    20281 tgtttaatcg ccgcaatgtt gagcagatga tctccacttg ggcaccggag gcggattcca
    20341 ttattagtgc tggctatacg ccgtctcgcg aagataaagt tgctgatttc gaagacacct
    20401 acaaccgcaa tctcaaagcg ttgatagaac ttcgtgataa ggcgtctgat cctaaagttg
    20461 tggcgcttta tacaaagaaa atcaatcaac tgattgctgc tggcaatacc gcgcttatta
    20521 aagagacggg gactgcgacg cagaagttgg cactggaata cgaaaacttg gcagagcaga
    20581 tcgaaagcac ctggactgat ttgtttagtg gcttaactga tgtcctgact gatttcgtta
    20641 ttaacgggaa gatgagcttc tccagcctat ctcagtccat tttgaaagat atcaccaata
    20701 tggtcgtgaa gtcgcaaatc acgctgcctc taatgaacat gttggggatg ggaaccaccg
    20761 cagctggtag ttcacagagt ggcaatttgc tcactggagt tgcttctgcc gttgccaatc
    20821 aaggtgtacg aatgggcaac actgttaatg gcgacaagtc ggtaggagaa gccacgaagg
    20881 agacgtccag ttcggtaact ggattggggc aaacaacaca gcagaccacc agcgcaattg
    20941 gcacagcaac aaatgcgatt ggtagctggg tatcggggct atttgatagc accgaagcca
    21001 aagatgccga gacaaaagcc gtgaaggact ccatcttctc gatgcagaac ctcagctctg
    21061 ttaccggcgc gctgtctgcc gcgtttgcaa tgcttggagc taatgcttcc ggctctggta
    21121 ataagtggtt gagttttgga gcaacggttg catctggtct tgtttcagct tgggctggcg
    21181 gtggtttcga cagcttgaca tcaagctctg ctaaaaccgc aaccagcagt gtggctgacg
    21241 gaactaaagg cattcctgca atcccgaagt ttgcaaatgg aggaatattc ggaaaagacg
    21301 gcgtgatccc gctccgggca taccagaaag gcggtattgc taactcgcct caattagcgt
    21361 tgtttgggga aggttctatg aatgaggcgt atgttccatt gcctgatggc cgaacaatcc
    21421 ctgtaacgct cagtaccgat ggtatgagtg gaggcggaaa tgttctttct cctgtatcaa
    21481 ttgagatcaa cgtccatagt gacggtagca caactgaatc cggcgataca gaaagcatat
    21541 ggaacaatgc cgctcaacgg atgaaagcaa tcgcgcttga gactatcgct caagagaaac
    21601 gccctggcgg atcactcaac ccaaacactc aacgtaacta actatcgact gccccgaccg
    21661 gggcagtctc acaaggatgt gagatggaaa gacaaacgtt taattggtat ccagattacg
    21721 aatctgaaaa aagcgtaaaa ccgaatgtaa ctgtacttaa ttttggtgat gactacgagc
    21781 agcgacaggc tcaaggtctt aatcgtatta aagaagaatg gtcgttaacc tttaccagat
    21841 catacaacga aattaatgca atcgatgact tcctgactga gcgatcaggc gttgaatcgt
    21901 tctattgggt taatccaaga ggcaagcaga ttgtagttgt atgtgacagt catacggtca
    21961 agcgatatca ggggtactgt gtcttaactg ctacattcag acaagtattt gaggcttaag
    22021 tatctggata agtaagtact aatttactat cattgtggcg ctgacaggat gttagcgctt
    22081 acttatttta aggatgaaac aatgggaatt aaagctgata ttcagagctt atctccctct
    22141 gcactcattg agttgttcgt attggatatg tcgaacacaa cttcaggggg gaagctattc
    22201 ttccacgccg gaacaaacga actgatgcaa ccggtcgtct ggcaaggagt gacatacgag
    22261 ccgtggccaa tcaaagcatc aggctttgac aaaactggcc agggaacgtt gccacgtcca
    22321 aaaattcagg tatcgaactt tgccggaacc gtctctgcgg aagttcaggc gaacgacgat
    22381 cttgttggct gccgcattat tcgcaagatg acgctggctc gcttcctcga tgccgttaat
    22441 tttaaagacg gcaacccaac agcagatcca aaccaacatt tcccggatga aatgtggttt
    22501 atcgaacaga aaactctcga aactcatcag gttgtcgagt ttgaattgtc cagtgtgttc
    22561 gatttgatgg gggtgcaact gccgtatcgt cagatcatta aaaacacctg cccgtggaaa
    22621 taccgagggc cagaatgcgg ctataccggt ccatatttcg acaaaaataa ccagcagacg
    22681 tctatgtctg gtgctgatta ctgcacaaaa cgttatgacg cctgtaatgc gcgtcggaat
    22741 tattttgccg acggtgtgat ccattttggc ggatttattg gagctacgcg gtatgggtaa
    22801 taaagcaatc cctgagcttg gctctgacgt tatgcagcaa atctatctct gcgccataaa
    22861 tcgctaccct aatgaagcgt gtggctttct ggttagaact aatggcgaca aatatcgctt
    22921 tatggaagcg cggaatgttt cggagaaccc gcagaacact tttgtaatgc acgttgacga
    22981 cattatggcg gcagaggatg cgggtgatgt tatcgcaatc tggcattcac atactgatga
    23041 atcagcagaa gcatctgatg ccgatcgtgc aggctgcgaa gcgacggaag ttccgtggat
    23101 gattctggct attcgcaaga atgttgaggg agatgcccct ttccatttta gcgagatgaa
    23161 tgtgatcact cctgatggtt tcgaaatgcc atacctgggc agaccgtatg tatttggcgt
    23221 atttgattgc tggatgttgt gtcgggacta tttgaagcgt gagtttaacg tcgaactaaa
    23281 cccgaacgca cacctgcata ttccatcgtg gtacaccggc gataacgaca ttctcgatca
    23341 gaactaccga aacgaaggat tggtacggct tgcgcccgga acagaacctc aacgtgggga
    23401 cgtcttcttt atccaatacg ggaaaatgcc tgaccactgc gcggtttata tcggcgatgg
    23461 aatgattctt catcaccaga tcgaccgcct aagttgtcgt gcttattacg gcggaatgta
    23521 tcagaaacat acgacgcatc acttgcgtca cagagacttg ctcaagggag atgagacgtg
    23581 tctgagttag ttcatgtgca gcttggcggc cctatggcca aacattttgg ccgccactgg
    23641 catctaaagg tgcgtaatac aaaacaggct ctggatttaa ttgaggccaa caagcctggg
    23701 tttaaagcat ggatgaagcg caatatcaaa acctatgaca gataccacat ccagatcacc
    23761 aataaacagg gccacaagtg gtctgtggac gagagtgaat atcagatgat ggggcagtct
    23821 gacaatattg caaaaatccg cattacccct gttccgcgag gaagtggtgg atctgctttt
    23881 ggatggtttc agacggttgt tggagcagta ttaatggttg cctcgatatg ggtgcccgct
    23941 cttgcgcctc tcggtttgtc actgatgatg ggcggtatag cgcaaatcat atctccgcaa
    24001 gccactaacg aaagtgtgag gcaggcggat aactcgaact cttattactt tgatggacct
    24061 caaaacacaa caaaccaggg gaaccctgta cagcttatct atggcgagga aattttagtt
    24121 ggctcacagg tcgtgagttc ttctatcacc atcgaccagc ttatgtaatc aaggattttt
    24181 tggacatgga acagttcaag aagaaaaaat taccgcttct tattgccggt gctggtggca
    24241 agaaaagtaa aagctccagc cgtaccccgg ttgaagctga tgatactgtc aattctcgtg
    24301 ctatggtagc catcctcgac cttcttggcg agggggttat cggtggcctg gtaaatggcg
    24361 caaagtcgat cttcatcgac gatttaccga tcttgaatga agatggctct tccaacttca
    24421 gcggtattac atgggatttt cgtgacggct cacaagatca gacgccaatg gctggttttg
    24481 attttgtcga aacgcctaag tccgtaaata ttcagcttaa aaaaacacac gacgttacgg
    24541 tttcaatcga taacgatgaa gctgatcgcg ttcgtgtcat catgaaattc ccctctttac
    24601 gtagcgttga taaaagctct ggcgatacaa atggcactac ggtgaagtac aaattccaga
    24661 tcgccaacgc agacgcaaca ttttctgatg tagtggcaga aggtgagaaa agcgttgata
    24721 tcacgctaac ggcaaagaaa accggcgtgt attaccgtag ctatgagctg aaactgccta
    24781 agcctggtcg tgcatacaag gttagggtga ttcgtcttac agatgataat agtagccagt
    24841 acctctacaa cgacacatgg gttgattcca tcggtgagat tgtcgacacg ccaatgaact
    24901 accctaactc tgctcttgtt ggcttgaagg tcaattcaga gcagtttggt agcacaatgc
    24961 cttcccgttc gtatttggtg cgcggtatca aaatccgagt accgtcaaac tacaacgaat
    25021 acaccaatac ttatgtcggc gtatgggacg gtacttttaa gctgttgtct tcttcaaacc
    25081 ctgcgtggat tctctacgat ttgcttacca atactcggta tggtcttggg caatttgtgt
    25141 cggaatccat gattgacctc ggccagctct accagattgg tcgatattgt gacgaagagg
    25201 tcgatgatgg ctttgggggt aaagagaaac gctttgcaat caatacgcag atcaccagtc
    25261 gacaagacgc ataccgatta attcaggata ttgccggtgc attccgcggc atggtgtttt
    25321 gggctggtgg catggttaac atcatgcagg atagcccatc agatccggta atgatgttta
    25381 ccaactccaa cgtcaaagat ggactgttta cctataaagg ttctgcgcgt aaagatcgcc
    25441 catccgttgc gctcgtaacc tacaacaaca aggaggacgg ttataagcaa aacatcgagt
    25501 acgttgaaga tcaggacgca atgcgccgtt atggtgagct taaaacagaa gtcgtagcat
    25561 ttgggtgtac aagccgaggc caggctcacc gagtaggttt gtggcttttg tataccgcca
    25621 gaatggagtc ggatgtaatt acatttactg ccggcttaga cgcctcattt ctgatgcctg
    25681 gtgaaaccgt tctgattcag aacaaatatc gtgcaggtaa acgtaactcc ggtcgaattg
    25741 tggagtttgc caaaaacagc atcactctcg atgcgcctgt gtcgttagct aaaagcggca
    25801 gctttattcg gatattgaat caggaaggca aaatcgttga acgcgatgtt cttgaaactg
    25861 gcgaaaacat aacaaaggtt acgttttcaa aagccctgtc gtcagcggaa acgcctgttt
    25921 tgaacggtgt ctggacaatt acagaaccag atctcgaacc tatgcgcgtt cgcatcgtta
    25981 acatcgcgca gggggaaacg ccgggtagct ttgacatcac cgctgttgag aacaatccgt
    26041 ctaaatatga ggcaatcgac aatggtgcaa cgctgatccc gcagaatacg acggtattgg
    26101 acccgactta ctccaagcca tctaatttgc aaatcactga agggacttat ctctcaagcc
    26161 cgggcaacct gtcagtaaaa ctgactgcaa catgggaagg gaaatctcca gagtattgga
    26221 tcagttggcg acgttctgat gaaaacaatg tatcgaactg gcaatcggcg cgtgtaaccg
    26281 aagagcaata cgaaatcgtt aatgtcgcgg agaatggacg ctacgacttc caactgtatg
    26341 cggtttcatt caacggtaaa aaaacagaga ttatcagtac cgtttatcag gtgttgggca
    26401 caatgacacc gccggatgcc ccaacgtcat taacggccgt tggagactat cgtaatgtga
    26461 tactgaattg ggttaatcca gattcggtag accttgatca cattaatgtt tacgcatccc
    26521 agaccaacaa tctggatacg gcgaaactga tcgcagagtc tgcaagcacc acctttacac
    26581 atgctggtct tggggatagc gaaacgtggt attactgggt tcgagcatcg aacaagcgag
    26641 gtatgttaag ccctccgaac tcaaacttgg gtacagaggc gacaactcgc gatgtactgt
    26701 cgttcctggc tggcaaaatt acatcttccg agcttggtca ggctctgctt gaggacatca
    26761 acagcaaagc ctctcaagaa gcagttgacg agttaaatga gcatatcaac cagagcgtcg
    26821 aatctttgga gggggcggta aacgacgtta aggaagatat agctgaatta gataagcagt
    26881 tcagcgataa cctcgcggat tttgaaataa aattcaacga gcgtagcgat gctttggaga
    26941 acgcacaaac cgagcttaaa ggtgaggttt cggcaacgat cgacaaggtc aatgaagcgt
    27001 ttgaaaaaat tgatgctact gatgctgcaa ttgttgaaat cgaaaacacc gtatctgaac
    27061 acgataaagc tctcgctaat acagtcgagg caataaaggc tgcaagagat gaagcggcgg
    27121 ctcttattgc taaggaaagc gaggctcgtg ttgaaggcga tgctgcaaac gctaaacaac
    27181 tggaagtgtt gcagtcaacg gtagaggaaa gctccgctgc cgttgaagaa atgaaaaaga
    27241 cggttgcaga ggtcgatcgt gccagtgcag aactgactac gaacattgag gcgttagcca
    27301 aaacaaatat tgacctggct cttcgtcaag atgaagacca gcacaagcag atggtcaata
    27361 acgcgaagat cgcaacaaca cagaaaacct ttgctgatga tatgtctgca atggccacga
    27421 aggttgagga aattcgcgta gaaattggcg aagacattaa ggccagtatt ctggaagagt
    27481 ccaccgctcg tgccgatggc gatgaagcgt tggcaaagcg tgtcactcag ctgcaatcta
    27541 agtttgaagg agatatcagc tcggcgatta gtacagagca ggaggctcgt acatctgccg
    27601 atgaagctct gacgacacag atcactcagc ttgagtcgaa agtaagcaac gatatcgtcg
    27661 cagctattaa ggaagagcaa gaagcccgag caacggaaga ttcggctctg gcaagccaga
    27721 tcacccaact tcaggcaaag gttgacggtg atatatccgc tgccatcact caggagcagg
    27781 aggccagagc aagtgctgac caggcgctct cacgagaaat taacagcctg cgagcacaaa
    27841 ccggaactga tattgccgca gcggttgctg tagagacaaa ggcaaggact gatgccgata
    27901 gtgcattgtc cactcagatt acgtctctta cagcaaaagc taatgatctc gaggcatctc
    27961 ttgccagaga aacaacggct cgtgctgatg gtgataccgc tttaacaaaa gaggtttcaa
    28021 gtttaaaagc acagacagcg aaagatatta gtgcggccgt tgcagttgaa acgcaggctc
    28081 gaactgatgc tgactcggcg ttgtcgtctc agatcaccaa gctgacctct caatacaaag
    28141 aggatattaa agcggctgta gcaaccgaaa cgaaaacgcg tacagagcaa gatgctgcat
    28201 tggcgactca aatcacaaac ttagagtctc aaacggcagc caacatttcc gcggcggtaa
    28261 caactgaaac gacagcgaga acgcaggcag ataatgctct gagtgggcga atcgatactc
    28321 tgaaagcaga ggtggatgga aataccgcga caattcaaca gcaggcaaca gctattgctg
    28381 ataccaacaa aaaagtgtca accgcgtgga cgttgaaaat ggaaacttca acgagcggag
    28441 ggcagaagta tgttgctggt attgcgcttg gtatcgacac cactggcctt tctcagtttt
    28501 tggtgcaggc tgacaggttt ggtttggtca actctgttaa cgggaagatc accacgccat
    28561 ttgttatcga aaacagtatc gcctatatga atggtgcgta tatcaaggat ggcacaatca
    28621 ccaatgcaaa agtaggcgat ctgcaatcta ccaattttgt tagcggtaga tctggatggc
    28681 ggttcggcaa aaatggaacg cttgagatca acggtaatag cgggggtaat gggcgactgg
    28741 tcataaatgg tcagcggatt gacgtttatg acgataacaa cgtcctacga gtaagaattg
    28801 gccttctgta atgtagaaaa aaatatttat atcggtaaaa ataagtaggt acatacttaa
    28861 tggtgggcaa ggatagccca ccaaatcaag gagcatattc aacatgtggt acagggaagg
    28921 tactatcaca tttacacagg gcagcaatac tctggttggg gcagggacag cctggaacgt
    28981 aacagctaat ggtgtgttgc cggggatgat cgtcattggc cccgacaata agctgtatga
    29041 gatcaagcgc gtaaccagtg atacgaacat tgttctctca gaaccttata ccggcgaaac
    29101 tcagtctgaa gttccgtgcc gaatcattac gacctatgaa ggcgacttaa cacagtttag
    29161 cgcgcgcttt acagcactaa tgtcgcgtat gtcggctgat tccaagtcca tgcgcagttg
    29221 gttgactgct ctggatgagg tgacaatcga gcgtgaagac ggtacagaag tgaccgttaa
    29281 gccgctaatg cagatcgtca acgagcacaa cgaaaacgtt gagtggtata aaaataacac
    29341 tgacgcgatt gatgctgctg gcgacaaagc tcgtgaggcg gcggccagtg ctgccgcagc
    29401 agcagaaagc gccaatatcg ctggagaaaa agcctctcaa gcgtctcaaa gtgcatccgc
    29461 tgcagagtcc tcaaaaagcg cggcggccac cagtgccggt gccgcgaaaa cgtctgaaac
    29521 gaatgctgca gcgtcacaaa aatctgcagc cacttctgca tccaccgcga ccacgaaggc
    29581 gtcagaagct gccacctcag cccgggatgc ggcggcctca aaagaggcag cgaaatcatc
    29641 agaaacgagc gcagcctcga gcgccagtaa tgccgcttcc tcggcaacgg cggcaggaaa
    29701 ttccgcgaag gcggcaaaaa cgtccgagac gaacgccagg tcttctgaaa cggcagcggg
    29761 acagagcgcc tcggctgcgg caggctcaaa aacagcggct gcgtcgtctg ccagtgccgc
    29821 gtcaacaagt gccgggcagg cctcagccag tgccaccgcc gccggaaaat cggcagaaag
    29881 tgccgcatcg tctgcttcaa cagccacaac gaaggctggc gaagccactg aacaggccag
    29941 cgcagcagcg aggtctgctt ccgcagcgaa gacatccgaa acgaacgcga aagcgtcgga
    30001 aacccgtgca gaatcctcaa aaacggcagc cgcatcgtcc gccagttcgg cagcgtcatc
    30061 ggcatcatct gcgtctgctt caaaagatga ggcgaccaga caggcgtcag cagcgaaggg
    30121 cagcgccacg acggcatcca cgaaagcaac agaggcggca ggcagtgcga cggcagcatc
    30181 tcagagcaaa actgctgctg aatccgctgc gacccgtgcg gaagctgctg ctgatcgtgc
    30241 tgaagagatt gccggtgcag ttgcgatgga agacgcaagc cttacaacta aaggtgttgt
    30301 gaaacttagc agtgctgtcg acagcaccag tgaatcgctg gccgcaacgc caaaagcagt
    30361 taaagtagtc aatgacaatg cgaacagcag ggtgccatct aaccgaaaaa ttaacgggaa
    30421 agcactgact gcggatatca cattaacgcc gaaagatatt ggtactttaa attcaataac
    30481 gatgtctttc tctggcgggg ccgggtggtt caaactggct actgttacaa tgccacaagc
    30541 gagttccatc gtttacatcg cattgattgg tggcgctggt tacaacgtcg gctccccaca
    30601 tcaggcaggc atttcagaac tggttctacg agcaggcaat ggaaacccca aagggattac
    30661 aggtgctttg tggaagcgta cagctgtcgg attaacgaat ttcgcctgga tcaacacatc
    30721 cggcgataca tatgatattt acgttgagat tggcaattat gcgactcgtg taaatatcca
    30781 ttgggattgt actgcaaatg cgacagtttc tatttataca tcgccaacat attcagctag
    30841 taagccttcc agcgttaccg atggtgttgt ttatacgatg tatagcacac atcagaaacc
    30901 gacgccgtta gatattggag cactgccaac aaccggagga acagtttcag gtccgttgtc
    30961 tgttactggt gggatcaccg gaacattaaa tggtaatgca agtacagcaa cgaaattgca
    31021 gacggcaaga tctatcggtg gagttggttt cgacggttct gcaaatatca accttccagg
    31081 tgtaaatact acgggtaatc agaacaccac tggtaatgct gcaactgcta caaaacttca
    31141 gacggcaaga actatcggcg gcgtgagctt tgatggtact gcgaatatta atttgccagg
    31201 tgttaatacg actggtaatc agaatacaac gggcaacgcg gctactgcta cgaagttgca
    31261 gactgcgcgt actatcaatg gggtgtcgtt tgacggctcg gcaaatattt ccttgtcgcc
    31321 agcaaatata ggttgcccgg catctcctac tggttggtta actacaggaa gtaatggcgg
    31381 agcaataaca acagcacagt tagtgacgtt attgcaaaat aatggagcat ttaacacaaa
    31441 gtcatggatt gctcgatgtg cttgggccta tgccaatagt gcaaccatac caaatagtga
    31501 aactggttgt ggcgttattc cattggcagg agctgttata gaggtattta ataacggtag
    31561 tagctcaaac aattatacga tccgtataac aacggccaca acgacgagtg tctctggtgc
    31621 tctcactaat gcggagttta tctatgtatt taatggcaca gattattctc cgggatggcg
    31681 aagagtatat aacacgaaaa acaaaccaac agcctctgat gtcggtgcat tacctcttac
    31741 cggtggtaca ttatctggag gtttgacatc ttctggcgag atcatttcaa aatatgcaaa
    31801 tggtttccgc attgcttacg gtagctttgg gttctttatc cgtaatgatg gatcgaacac
    31861 atatttcatg ctaacagcat caggagacac attaggttca tggaacggtt tgcgacctat
    31921 tacaattaat aataccagcg gtgctgtatc aattggtaat ggactaaatg tgactggtgg
    31981 cgtaaatggt agtttgaacg gtaatgcttc aacagctacg aagttgcaaa cagcgagaaa
    32041 catcaatggt gttaagtttg atggctcagg cgatatcaac attaatacat tggtatctcg
    32101 tggccgagtt acggcgttaa gcggctctac tcaaggcact gctggcattc aaatgtacga
    32161 ggcgtacaac aatagctacc cgaccacgta tggcaacgta ttgcacatga aaggtgcgag
    32221 tgctgctggt gagggcgagt tgcttattgg ctggagtggt acgagcggtg cacatgcgcc
    32281 agttttcatt cgctcacgaa gagataccac agatgcggca tggtcagcgt gggcgcaggt
    32341 atatactgct aaggattcaa tccctggtgt gaatacaacc ggtaatcaga atactactgg
    32401 taatgccgca acagccacaa aattgcagac agcaaggaaa attgctggtg tggcgtttga
    32461 tggctctgcc gatattactt tgactgcggc taaccttaat gcttatacga aaacagaggt
    32521 aacaaacctt ctaagttcct atgcaagcag atcatcactg acaggctata gtggcaacct
    32581 ggatattatt gctgaaacac tggttgtcaa atcaggcggt agtggagggt ttgctatatg
    32641 ggatattggc acaactacta gcggtgccaa tatgtacatt gatccaaacc ctggtatcaa
    32701 tacagtttgg cgttcaacat cttcaaggcg ctataaaaag gatattgaaa cattacaaga
    32761 tcgatatgct gatgaacttt tgtcattaag acctgtttgg tatcgttcaa tttgtcgagg
    32821 tgaccgaaag gattgggggt attacggcct tattgctgaa gaggttggtg agattgcccc
    32881 gcaatatgtc cattggcgtg aaccaacaaa taatgattct ccagaagata tttcctcaaa
    32941 tggtatggtc gctgaagggg tgatgtatga gcgtttggtt gtaccactca ttcatcatat
    33001 tcagcaattg accaaaaggg ttgaggagct tgaaacgaag ttaaattcac ctaaagaata
    33061 aacaacttca atcggggctg gaactaaacc agcccctgct taaaagtata aggatatctt
    33121 tatgagctat ggtgcacagg tatggtcgcc atcaagacag gagatggtcg atgcgttggc
    33181 ccctgtttat tacctcgatt attttactcc ctctggttct ggaagtagaa catatgaggt
    33241 tgaggcggga ttgggaatcg actattacat aatggacgta accaatggga aatacacaag
    33301 tcttacggtt tctggaaata ccatttcatg gtctggagct agtggcaatt taaacatatt
    33361 ggtttttcaa aaataatgta cggaagcaag atatatcgat ctgacgggaa ggtctggatg
    33421 tctccttctt tgtctcctat cgtttttcag agaaagcagg ttgtttcttt gtctggcggg
    33481 acagagttta acacccaaat atctccagat cgttcaccga tgatttttgt tgcctattcg
    33541 aaagctgttt cattaatagc caacagaata gtgcgtaaca accaggtgat ttacagtttc
    33601 ggaggtcagg gtagcgactc tagtgccacg atatacgtat tttcgaaagg tatagccaaa
    33661 aaagaaactt ggggcatgag ttttttcaat gcacaagggg aggaaatcta taatacggca
    33721 aatatcccac tttcatttac ctttcttaat aatacggagt ggaacagctc tggagggcat
    33781 gtttttgatt acccaccagc gataatccca acatatgcga atgtgttcgc tgttccagta
    33841 ccgggcggag ccatgacaat ggtttatgga tatgcggcat atggaaatac tgttagctcg
    33901 atattcgtaa accaacttaa tggcggccat agttttagcg taaatggcag agtaccggta
    33961 ataaatagaa atctgtataa ctgatgagta aatgatgaaa agattatttc taacaatagc
    34021 tgttgttctc tctcttactg gctgccagaa attgccgaca cctatatgtt acggtgaggc
    34081 tatcattggt ggacaggaga cagtcatacc catatatgct gttaaaaaag tgaacgacta
    34141 taccttatac aaagcgggta gcatctataa ctggcgatgg gtaggagtag gtgctttctc
    34201 atcagtccgt tgcaatgttg aatgataagt aagtacacac ctacaacaag aaggtgaaat
    34261 gtgatataaa tccgccatcc cgatttgact tttcatggag gaaaacatgt cgaacgagat
    34321 ggcgggcgtt acaacagagc aagttgagcg tattgccgcg atcgttgctc gggaggttgt
    34381 tggcaaatta ggtaaagagc tacgtgaaga aattggccag gaggtcaatg atcagctgaa
    34441 aacctacttt ggtgatatga ccccggcgca acatagtatt caacactcca acctggacaa
    34501 actccttaac cggttagatt ccatctccag tgggttcttt ggcggcattg tttctaaaat
    34561 aacgtcgttc attattactg cactgctttt ggggttagcc gcgtatggcg taaaaaatgg
    34621 actgcaataa caggagatca aggatgaaga ctccgagagg cattcgtaat aataaccccg
    34681 gtaatcttga taaaggatca ccgtggcaag gtctggttgc gaatccagac gaaccgcgct
    34741 tttgcacgtt taaagaccct gtttggggga ttcgtgcgct ggcggtgact ctaattacct
    34801 accacgacaa acgtcgcgca aaagacggct caagtatcga taccattcgt gaagttattg
    34861 aacgttgggc accgccgaat gaaaacaaca ctgacgccta cattaatgag gtgtctaaag
    34921 ccgttggtgt aaccgcagac atgatcatcg atctgcatga ttacgacatc cttcgacctt
    34981 tggttgaggc aatcattcgc cacgagaatg gtcgaggtcc actaaaaacg ctgaacacct
    35041 ggtatgcggc agaagttatt gaggaaggtc tgcgtcgagc tggcgtcgtt aagccggtga
    35101 aaaccgtgaa ggctgttcct gtaactaaag aaaccgctgg cgcaactgtt acagcaggta
    35161 ttggtctggc gcagctggcc gatgttatgc cgcaggtttc cgctgctatg gataaagcac
    35221 aaggtcatat ctctagcggg gatacagtac gcatcatctt cggtattgcc actattgttg
    35281 tggctggatt tatcgcctgg tcgcaggtaa gaagacacca gaaagggatg gtctaatatg
    35341 ctaggcagcc tgatgacaaa gctaaaagtt gctttgatta cgctggctgc cgttcttttc
    35401 gttcttgtcg gcgcttacac gatgggcgga aatgcggcgc gacgagcaat ggaagagaag
    35461 gcaaaacagg aagacagaaa acgacttcaa agcacagtgg atgtcaaaaa tgaaacggtt
    35521 aatgaagtgc ggcaaaaaga tgcctctggt gttcatcgcg agctgcttga taaatgggtg
    35581 cgtgattaag acgcaggctc caggcgtgct attctgcgat gctgcaaatc ctatctatgt
    35641 gagtagagag gattttatga ctgaggaaac ggagcgacag ttgcttatcc acaatacgat
    35701 aggtgaaagg ctgtgtgagt gggggcacct gaactgaggc gatgattgat gagtggtctc
    35761 agtttacctt ttatcctaaa gatggcatct tcactcatat acacgcctgt tcactcaagc
    35821 tcatgttgaa tcatttagta atttgatgta gatttattag gcatccacgt aagtgggcac
    35881 atacaggcta cgcatcctcg tagccttcaa ccccgagagg ggtgccgcaa tgacacttgt
    35941 gttaacggct aaggtggaca catgctgaac gagaaacgtt ctatacaaac tattcgcgat
    36001 tgcacgagta ttatcgagac tcatgtgctg aatcagcaca agcttgaagt acgtaagctc
    36061 aataatgacg ctattgaacc ggaagactca cgcttcatcg ttaccttcaa agacgatgtt
    36121 gagcgcgctg gtgcaagcgt gaagccatta gctgaagcaa tttacctgaa tggtcgtgtt
    36181 cgttttgtag tcaagccagc taaacagtac cctgagctgt ctcgttttat cgaggctttt
    36241 tcggaggtta ttgaacattc tattcgccgc ttcttacgca atcatggtag cattgtcagc
    36301 acggttaagg ctgatgattc tgctacaaaa tggctcctac actagatagc gcatattcaa
    36361 aagatctaag cgagtttcct cataaagggg agactcgtgt tgttcgtttt ggctttctta
    36421 tcaatgaagc ctccctctat aaaatctcag aaattgaaat cattgaacca gaagatgaca
    36481 tttgcctgta tgtctctatg gaacgtgtag gtgcgcgcga ccaaggcgac ctgagcgaat
    36541 tcatcttaga tcgagcggat gaagatgccc ctgaagaaga gattatcaaa gaggtgctcc
    36601 agtccgggct tctggacgaa aacaaaaata cgatcgctgg taggatagct ctgcgtgaat
    36661 atagttttgt tgaagacggt aatgagatcg aatgttacca ggtagctggt gtcgaaactg
    36721 tcagagagcg tcgtcagcga ggattgtgtc accgtaccta tctctttctt cttcattggt
    36781 atgagcattt agtctgcgac gatacacaaa ccattcctgg tgctaagatc tgggcagggc
    36841 ctctcatgcg aaccggcgat gtgcgtatat acaatgccaa aacagaaacg ttcgaagatg
    36901 tgttaggtga gtacgggatg gggaaagaaa ccggattttt gccgtggaac agaggattgt
    36961 tgcttgatgc agagttaagc tcttggctcc caaacaaagt gcaagttaac gttcagaaat
    37021 ttatcgtact tatcatctcc cgcaaaacaa gaactccagt aggtttatac ctaaaagatt
    37081 aaaaatcagg cgactttggt cgcctttttt gttaacagcc attaattctc gcctaaaaat
    37141 tctaaggcag cacctaccat ttaaccttta caccacagcc gtaggcattt aggctatatc
    37201 acatataaga aaacaagttg tttcagacga taattatata cgcaaaggga actctccaat
    37261 gaccaagatc tttgtggttg gcggcacaaa gggcgggcct ggcaaatcca ccgttgccca
    37321 gcaaattgcc gtttgcctga aagtcaaaaa gaagaagaag gtttatatta ccgatataga
    37381 tattcagcgc acgacaacga gctggtgtga agaccgtcga cagaacgaag accttgagct
    37441 gattcctttt gcatacgttc aggatgacat cattaagcac ctaaaatcgc ttcagggtag
    37501 agctgagttt gtagtggtag atgctggtgg cttcgactcc gaaattcagc gacaagcgat
    37561 gctgatggct gacgttatca ttatcccgct gcgtcctaag cgtcgtgatt tgaaatcttt
    37621 acgtgacatc gatcctatta tcgacaatgt tcgcaatgta aacgataaag tgaaggtccg
    37681 cgcggtcatg aaccagtgcc cggctttgcc atcacaagtg tctcgcattc tggcggctaa
    37741 agagattgtt gagacgtttg gcatcgagtc tgcgccagtc aatctatata accgcaacgt
    37801 ctatgatgat gcggaagagt ctggtcgttc tatctttgaa atgaccggta gcgagcgcga
    37861 caaaaaggcg gaagccgagt ttgaagaatt tgtagattat ctgttgagtc tggaggaaga
    37921 agaataatgt ccatgaaaat gggtgaccta gcaaagcgca aagagcctga tgcaccggct
    37981 aagaacacaa ctcctttgcg ccaaccagtc agaccacagg gacgcccgac tcgtggcaaa
    38041 gagaaaatta aaagccgcac aatgtcactg gaagacgaat acttcgaact gctggagatg
    38101 atgaagttca tccctcgctt cgagaagttc actcgttctg acgtgattcg agcagccatt
    38161 ttccatctgg cagagaagtc accgcaggaa atcgaggaca tcgtgaagct taacgaggcg
    38221 atcacagctg ccgacgttac gatgcgtacc gatgaaatta aacgagagtt gatgaagaaa
    38281 ggttgattat attgggcgtt tgttacgccc aatatatgaa ttaaaggatt gatactaatg
    38341 gaatcgatag aatcatttct aaaaaatttt ctagtttcag atcatcgggt tgccgtcata
    38401 aaaggagatt ggggcgttgg taaaactcat tattggaata gtttttatac taagcattct
    38461 gaaggattag attttaatgc atattcgtat gtttctttgt ttggggttaa ctctattggt
    38521 gatattaaaa aag
//
LOCUS       DAZFZC010000039        38528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFZC010000039 DAZFZC010000000
VERSION     DAZFZC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.039"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(214..405)
                     /gene="yfgG"
                     /locus_tag="ACHKXP_002321"
     CDS             complement(214..405)
                     /gene="yfgG"
                     /locus_tag="ACHKXP_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO7647311.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            423..575
                     /locus_tag="ACHKXP_002322"
     CDS             423..575
                     /locus_tag="ACHKXP_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647312.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            757..3000
                     /gene="pdeF"
                     /locus_tag="ACHKXP_002323"
     CDS             757..3000
                     /gene="pdeF"
                     /locus_tag="ACHKXP_002323"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO7647313.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMLVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(3039..4580)
                     /gene="ppx"
                     /locus_tag="ACHKXP_002324"
     CDS             complement(3039..4580)
                     /gene="ppx"
                     /locus_tag="ACHKXP_002324"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO7647314.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4585..6651)
                     /gene="ppk1"
                     /locus_tag="ACHKXP_002325"
     CDS             complement(4585..6651)
                     /gene="ppk1"
                     /locus_tag="ACHKXP_002325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO7647315.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6823..7461)
                     /gene="purN"
                     /locus_tag="ACHKXP_002326"
     CDS             complement(6823..7461)
                     /gene="purN"
                     /locus_tag="ACHKXP_002326"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO7647316.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7461..8498)
                     /gene="purM"
                     /locus_tag="ACHKXP_002327"
     CDS             complement(7461..8498)
                     /gene="purM"
                     /locus_tag="ACHKXP_002327"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO7647317.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8823..9449
                     /gene="upp"
                     /locus_tag="ACHKXP_002328"
     CDS             8823..9449
                     /gene="upp"
                     /locus_tag="ACHKXP_002328"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO7647318.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9535..10824
                     /gene="uraA"
                     /locus_tag="ACHKXP_002329"
     CDS             9535..10824
                     /gene="uraA"
                     /locus_tag="ACHKXP_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO7647319.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10919..11620
                     /gene="hda"
                     /locus_tag="ACHKXP_002330"
     CDS             10919..11620
                     /gene="hda"
                     /locus_tag="ACHKXP_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO7647320.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11758..12117)
                     /gene="arsC"
                     /locus_tag="ACHKXP_002331"
     CDS             complement(11758..12117)
                     /gene="arsC"
                     /locus_tag="ACHKXP_002331"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416990.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO7647321.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKILGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12138..13601)
                     /gene="bepA"
                     /locus_tag="ACHKXP_002332"
     CDS             complement(12138..13601)
                     /gene="bepA"
                     /locus_tag="ACHKXP_002332"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO7647322.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13814..14875
                     /gene="yfgO"
                     /locus_tag="ACHKXP_002333"
     CDS             13814..14875
                     /gene="yfgO"
                     /locus_tag="ACHKXP_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7647323.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14913..15761)
                     /gene="focB"
                     /locus_tag="ACHKXP_002334"
     CDS             complement(14913..15761)
                     /gene="focB"
                     /locus_tag="ACHKXP_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO7647324.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPPPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15783..17795)
                     /gene="hyfR"
                     /locus_tag="ACHKXP_002335"
     CDS             complement(15783..17795)
                     /gene="hyfR"
                     /locus_tag="ACHKXP_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO7647325.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17825..18238)
                     /gene="hyfJ"
                     /locus_tag="ACHKXP_002336"
     CDS             complement(17825..18238)
                     /gene="hyfJ"
                     /locus_tag="ACHKXP_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO7647326.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIT"
     gene            complement(18231..18989)
                     /gene="hyfI"
                     /locus_tag="ACHKXP_002337"
     CDS             complement(18231..18989)
                     /gene="hyfI"
                     /locus_tag="ACHKXP_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO7647327.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18986..19531)
                     /gene="hyfH"
                     /locus_tag="ACHKXP_002338"
     CDS             complement(18986..19531)
                     /gene="hyfH"
                     /locus_tag="ACHKXP_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO7647328.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTIALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVLLVAKEQL"
     gene            complement(19541..21256)
                     /gene="hyfG"
                     /locus_tag="ACHKXP_002339"
     CDS             complement(19541..21256)
                     /gene="hyfG"
                     /locus_tag="ACHKXP_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO7647329.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVQYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIAREYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21246..22825)
                     /gene="hyfF"
                     /locus_tag="ACHKXP_002340"
                     /pseudo
     CDS             complement(21246..22825)
                     /gene="hyfF"
                     /locus_tag="ACHKXP_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            complement(22830..23480)
                     /gene="hyfE"
                     /locus_tag="ACHKXP_002341"
     CDS             complement(22830..23480)
                     /gene="hyfE"
                     /locus_tag="ACHKXP_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO7647330.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23492..24931)
                     /gene="hyfD"
                     /locus_tag="ACHKXP_002342"
     CDS             complement(23492..24931)
                     /gene="hyfD"
                     /locus_tag="ACHKXP_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO7647331.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24948..25895)
                     /gene="hyfC"
                     /locus_tag="ACHKXP_002343"
     CDS             complement(24948..25895)
                     /gene="hyfC"
                     /locus_tag="ACHKXP_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO7647332.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25906..27923)
                     /gene="hyfB"
                     /locus_tag="ACHKXP_002344"
                     /pseudo
     CDS             complement(25906..27923)
                     /gene="hyfB"
                     /locus_tag="ACHKXP_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27923..28540)
                     /gene="hyfA"
                     /locus_tag="ACHKXP_002345"
     CDS             complement(27923..28540)
                     /gene="hyfA"
                     /locus_tag="ACHKXP_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO7647333.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKNGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28793..29263)
                     /gene="bcp"
                     /locus_tag="ACHKXP_002346"
     CDS             complement(28793..29263)
                     /gene="bcp"
                     /locus_tag="ACHKXP_002346"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO7647334.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29263..29835)
                     /gene="gcvR"
                     /locus_tag="ACHKXP_002347"
     CDS             complement(29263..29835)
                     /gene="gcvR"
                     /locus_tag="ACHKXP_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO7647335.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29981..30859
                     /gene="dapA"
                     /locus_tag="ACHKXP_002348"
     CDS             29981..30859
                     /gene="dapA"
                     /locus_tag="ACHKXP_002348"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO7647336.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30876..31910
                     /gene="bamC"
                     /locus_tag="ACHKXP_002349"
     CDS             30876..31910
                     /gene="bamC"
                     /locus_tag="ACHKXP_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO7647337.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32123..32836
                     /gene="purC"
                     /locus_tag="ACHKXP_002350"
     CDS             32123..32836
                     /gene="purC"
                     /locus_tag="ACHKXP_002350"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO7647338.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            33004..33867
                     /gene="ypfJ"
                     /locus_tag="ACHKXP_002351"
     CDS             33004..33867
                     /gene="ypfJ"
                     /locus_tag="ACHKXP_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO7647339.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33882..35897
                     /gene="tmcA"
                     /locus_tag="ACHKXP_002352"
     CDS             33882..35897
                     /gene="tmcA"
                     /locus_tag="ACHKXP_002352"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO7647340.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35971..36669
                     /gene="ypfH"
                     /locus_tag="ACHKXP_002353"
     CDS             35971..36669
                     /gene="ypfH"
                     /locus_tag="ACHKXP_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7647341.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36750..36950)
                     /gene="ypfN"
                     /locus_tag="ACHKXP_002354"
     CDS             complement(36750..36950)
                     /gene="ypfN"
                     /locus_tag="ACHKXP_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO7647342.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36978..38105)
                     /gene="dapE"
                     /locus_tag="ACHKXP_002355"
     CDS             complement(36978..38105)
                     /gene="dapE"
                     /locus_tag="ACHKXP_002355"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO7647343.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMSRNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38109..38465)
                     /gene="yffB"
                     /locus_tag="ACHKXP_002356"
     CDS             complement(38109..38465)
                     /gene="yffB"
                     /locus_tag="ACHKXP_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO7647344.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLND
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCVPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9429 a         9915 c        10096 g         9088 t
ORIGIN      
        1 aaaattcacg cagggaataa tttataaaaa tatatcatta caacatatat attaatttta
       61 tatttatttc gcagccaaac aggataaaca tcagtcagga taaagacgta aaatttaaga
      121 tgaaagaata tctgccgctt tgataatccc aaacagcaaa agcatccgac agcgaagtgc
      181 cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga
      241 gagtgtggat tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga
      301 gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg
      361 actgttaaat cggtgtcgtt ttcgcatact ggttgcctga ctcacaaaag gttccttgaa
      421 gtatgtccca cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt
      481 cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa
      541 ttatttacaa tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa
      601 tcagggttat tttgtttgat atatatcaat tggtactttc atatgacact tagaatcaat
      661 gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca
      721 cctccttcag gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata
      781 aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt
      841 ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg
      901 cctctggcac tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc
      961 gccttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac gctatcatta
     1021 acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc
     1081 cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag
     1141 attgtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta
     1201 ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat
     1261 tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg
     1321 gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat
     1381 gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg
     1441 ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca
     1501 ttattgctgc ccctgatgat gtggggagcg atgcgctatg gttataagct gatttcgctg
     1561 ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat
     1621 cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt
     1681 gtcaattata tggctgtact ggcaacccgt cagagagcgg tagtcagacg cattcagcgg
     1741 cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg
     1801 cgtgatgccc cctggtctgc gctttgttat ttacgcatcc ctggcatgga aatgctggtt
     1861 aagaactatg gcatcatgct gcggattcaa tacaagcaaa aactttctca ctggctgtca
     1921 cccttgctgg aaccgggtga agatgtttat cagctttcgg gtaacgatct cgcgctgcga
     1981 ctgaatacag aatcgcacca ggagcgcatt accgcactgg atagccatct caagcaattt
     2041 cgtttctttt gggatggcat gccgatgcaa ccgcagattg gcgtcagtta ctgctatgtg
     2101 cgctcgccag tgaatcatat ctacctgctg ctgggagagc taaatacggt cgccgaactt
     2161 tccatcgtga ccaacgcccc ggaaaatatg cagcgtcgcg gggcaatgta tttgcaacgc
     2221 gaattgaaag ataaagtcgc gatgatgaat cgactacagc aggcgctgga acacaaccat
     2281 tttttcctga tggcccagcc gattaccggt atgcgtggtg atgtttacca tgaaattctt
     2341 ctgcgcatga aaggtgagaa tgatgaactg atcagccccg atagcttctt gccggtcgcg
     2401 cacgaatttg gtttatcgtc gagtatcgac atgtgggtca ttgagcatac gctgcaattt
     2461 atggctgaaa acagagcgaa gatgcccgct caccgttttg ctattaatct gtctccaacc
     2521 tcggtatgtc aggctcgttt tcctgttgaa gtcagtcagt tgctggctaa atatcagatt
     2581 gaagcgtggc aacttatttt tgaagtcacc gaaagtaatg ctctgaccaa tgttaagcag
     2641 gcgcaaatca ccttgcagca tcttcaggaa ttaggctgcc agattgcgat tgatgatttc
     2701 ggcaccggct acgccagcta tgcgcggctt aaaaatgtga atgccgatct gcttaaaatt
     2761 gacggcagtt ttatccgcaa tattgtgtca aatagtctgg attatcagat agtggcatcg
     2821 atttgccacc tggcgcgaat gaagaaaatg ctggtagtgg cagagtacgt tgaaaacgaa
     2881 gagatccgcg aggcggtgct ctctttgggg atcgattata tgcagggtta tcttattggt
     2941 aagccgcagc cgttaattga tacgctgaat gaaatcgaac ccattcgcga aagtgcctga
     3001 ataatgcggg ccgacatttc tcgtcggccc gcaaagtatt aagcggcgat ttctggtgta
     3061 ctttcttctt caattttcaa ccgccagcca gccacgcctt cccagtattc ttgctccttt
     3121 tccagatcaa gcagtaccag cgcattctga ctaaaccagt catgcgggaa acgcagtgtc
     3181 cagtgactgt catcagtaat cagcgtcaat gttggcggtg tggtggttgc ctgacgttga
     3241 ttgttgagta atacgccaag gcgcaatagc tgtatcagtg gcaggaactg tttcttctta
     3301 aacaaggtaa agcgcggcag atcgtcgagc ttaatcgctt tacggtgata gcgcaccagt
     3361 gtcgccatca tcagctgctg ttcctgatta aaacccggca agtcactgtt ttgcagaata
     3421 taagcggagt ggcgatgcaa accgctgtgg ttgatattca acccgacctc atgcagcatg
     3481 gcggcccatc gcagtagcgc ctccagttgc ggatgcgcca gcttcggttg ctgttcccgc
     3541 cactgttcgt acatttgcat agtggtatcc agcacccgtc gggcctgttc gctgtcgatg
     3601 tgatactggt tggcgaggct gctggcggtg cgactacgca catcctgatg acggaaacgt
     3661 ccttccattt catacagtac gccttcgcgt aacgccccgt cagaaaggcg cagttcacgg
     3721 atggctaaag catcaaacac accgcataaa atcgccagtc ccggaacgaa gactgttttc
     3781 cgctcttcgg aaagacccgg taaactcagc gatgcgaaat tacggtgacg taaaacttct
     3841 tttaccagtt tttccagacg ttccggggta attatcccgt ctttctcgcc catttccatt
     3901 aacacttcat gggcggcttt tatggtaccg gaagcgccca ttgcaacgtt ccagccctga
     3961 atacggaatt gccaggttaa agtttccagt ttttgtgctg ccgccatgcg agcgcgctga
     4021 aaattctctt tattgatgac cccgccagga aaataaagct gggcaaagct gacacaaccc
     4081 atccggcggc tttcaacgag aataggttcg aaattttcac caatcaccag ttccgtagat
     4141 ccgccgccaa tatcaataac cagtttgcga cctttttccg gttgggtatg ttccacgccc
     4201 ataaaaatca gacgggcttc ttcattaccg gaaataattt caatcgggta gggaatgacc
     4261 ttttccgcgc gtttcagaaa gtcagtggcg ttcagcgcct gacgcagcgt atgggtacca
     4321 actatacaga cgctggcagg agaaaaccct tgtagccgtt cggcaaacag cgacagacag
     4381 tttaaaccgc gcgtcattgc ctcttcactc aacatattat ctggccccag gccgtccgcc
     4441 agatgcaccc gctgtttcag gcggccaata atctgcatgg caccatctac cacacgggct
     4501 atgaccatgt gaaaactgtt tgaaccaaga tcgaccgcag caaactcctg cggacgaggg
     4561 gatttatcgt gtattggcat agggttattc aggttgttcg agtgatttga tgtagtcata
     4621 aatcgccaac tgcgcccgta ctttgcggcg attgccgcgg ggaacgtagc gattactgag
     4681 ttctttatcg atataacgtg ctttgaccgt atcgctgaac aatatgtcga tgatgtccag
     4741 tacccgctgc ttcaggcgcg gatcgagcag cggcgtcgcc acttcaatac gataatcaat
     4801 attgcgcgtc atccagtcgg cggaagaaag gtagaccttt ttatcgccgc cattttcaaa
     4861 aatataaacc cggtcatgtt caaggtaacg gtcaacaata ctgatggcac gaatgttgtc
     4921 gctaatgcct tccagattgg ggatcagcga acacattccg cgaaccagca gattaaccgg
     4981 tacgccggag ctggaggccg catacagacg atcaaccagg cctttatcga caaggttatt
     5041 tagcttcagg gtgataccac tgggcagccc ttgctgcgcg ttggcgatct cgcggtccac
     5101 catttcatac aataggcggc gggagttttg cggcgatacc attaaataat caaatgtcac
     5161 cggacggtat gggttttcaa taaagttaaa tacccgccgt acttcgttgg tgatgcgcgc
     5221 atcggcggtc agcaacgaat agtcagtata aagacgcgcg gttttttcgt taaagttccc
     5281 ggtcccgatg tgtgcataac gcaccacttc accgttttct ttacgtgaaa tcaggaacag
     5341 tttggcgtga attttcagcc ccggcgcaga gaagataacg tgcacgcctg cttcggtcag
     5401 gcgcttcgcc cagtgaatgt tggcttcttc gtcgaaacgc gcctgtaact caaccaccac
     5461 ggtgactttc ttaccgttat gtgcggcgtg gatcatcgag tcgatgatgc gtgaatcttt
     5521 cgccacgcgg taaatgttaa ttttaatcgc cagtacgctc gggtcgaacg aagcctgacg
     5581 cagcagttcc agcacatgct caaaggtgtg ataaggataa tagagcaaca catcgcgttc
     5641 gcgaatggca tcaaaaccat tgcggaactg ggctttatca aaccaaatat ggcgtaaacg
     5701 cggcagtggt ttgttcacca gattggcttt gccgacattg gggaaattaa taaagtcttt
     5761 aaaattatga taacgaccgc cggggacgat ggagtcgtag cgggaaatag tcagtttttc
     5821 gcgtaacact tcaaccagcg cattgggcat atcgcgctga taaacaaaac gcaccggctc
     5881 agcagttaaa cgctgcttga gactggaaga catcaactcc atcaggctgg cttccatctc
     5941 atgcactaaa tcgtattcgg catcgcgggt catcttcatt gaataggcat tcagcgcgtc
     6001 ataatcaaag aagcctttga aaatatcatc aaggcagtaa cgcagaatgt tatccagaag
     6061 aatcatcggc ttgcgtcgac gcggtgcttc tggcggtaaa ttcacaaagc gcggcacttt
     6121 atctgatggg atctccagca gcgcgtaacg gatggtatcg ccacggataa tttccaccgc
     6181 cagataggtg taatcatctt tcaggaactg cactaagtca gtgtcaggat tgattaaaat
     6241 cggcgtaatg tgctgacgca gatactgctt aaaataatga cgcagccagt tttgttgatt
     6301 gacggagagc tggcgttcat taatcaggaa gatctggttg cgcgccatct ccagcaatag
     6361 ctcgttgtag aggccgtcga attcctgatc ggctttcagc acccgggact gaattttgcc
     6421 cagtaaatgg cgggaatgag agttggagcc ttgttcttcg ctaataatga tgcgtcgctt
     6481 cagttcagcg aagcggactt tatagaactc atcaaggtta ttggaataga tccccaggaa
     6541 acgcatcctt tcaatcagcg ggttagattt gtccgccgct tcctgaagca cgcgttcatt
     6601 gaacgataac caactgagct ctttttcgat gtatagcttt tcctgaccca ttaccacttt
     6661 tactccgttt tattcacggg acactgcctg gatattatgg cgagcaatat tggcagatgt
     6721 ccaactatgc cagaaaaggg ggaaaaaaac tgctggagct tgcgatttac aggtgaattg
     6781 aaaaacgcca gcggcagagc tggcgcttta actacggggg cattactcgt cggcagcgta
     6841 gccctgcggc ggcagacgtt gaccatccag ccacgcggcg ttttcgtgca ttttcagacg
     6901 accatcggca aaccagctaa tcaccagtgg ataaatagcg tgttcctggg tttgcacgcg
     6961 ggcggtgatg tcatcttccg tatcaccagc aaataccggg actttcgcct gtaaaataac
     7021 cgggccaccg tccagttcat cggtgacgaa atgcaccgat gtaccgtgct cttcatcacc
     7081 attttccagc gcctgacgat gggtgtgtaa tccgggatat ttcggcagca gagaagggtg
     7141 aatgttcagc aaacgcccgg catagtggga gacaaacgcc gggctgagaa tgcgcataaa
     7201 accagccagc acgaccacat cgggtgcgta catgtcgatt tcatgaatca actcccggtc
     7261 ataggcttca cgactgtcaa acgcgctggc gatgagcgta tgcgttgcaa tacccgcctg
     7321 gcgggcgcgt tcaaggccga acgcgtcggc cttattgctg aaaactgccc gtacggtgcc
     7381 tttaattttg ttagttttac aggcgtcaat aattgcctgt aaattacttc cgttgccgga
     7441 aataagcacc acaatattca ttattcgata accacgcgtt gttcggaatc agaggctttg
     7501 atgataccga ttttccacgc gttttcaccg ttggcattta gcagggcgag ggctttgtcc
     7561 acttccggag caggcagggc aataatcatc ccgacgccgc agttgaaggt gcgatacatt
     7621 tcatggcgtt caacgttacc tgccgtttgc agccagttga acacttccgg ccactgccag
     7681 gaagattcat caatcactgc ctgggtatta tctggcaata cgcgcggaat gttttcccag
     7741 aagccgccgc cggtcaggtg cgcaatggca tgcacatcga ccttttcaat caactccagc
     7801 actgacttca cgtaaatgcg ggtcggtgcc agcagatgat cggctaatgg tttaccatca
     7861 agttcggtgg tttgcggatc acaaccgctg acttcaagaa ttttgcgcac cagcgaatag
     7921 ccgttcgagt gcggaccgct ggaaccgagt gcaatcagca catcgccgtc gctgacttta
     7981 gagccgtcga tgatttctga tttttctacc acgcccacgc agaaacccgc gacatcgtaa
     8041 tcttcaccgt gatacatccc tggcatttct gccgtttcgc cacccaccag tgaacagccc
     8101 gattgcagac aaccttccgc aatgccgctg atcaccgctg aagcggtatc aacatccagt
     8161 tttccggttg cgtaatagtc gaggaaaaac agcggttctg caccttgcac caccaggtca
     8221 ttaacgcaca tggcgaccag atcaatacca atggtgtcgt gacgttttaa gtccattgcc
     8281 agacgcagct tggttcctac gccgtcagta ccggaaacca gcacaggttc acgatatttt
     8341 tgcggcaatg cacacagcgc accgaagccg cccagaccgc ccatcacttc cggacgacgc
     8401 gttttcttca ctacgccttt gattcttcca accagagcat tacccgcgtc aatatcaaca
     8461 ccggcatctt tgtagctaag agaggtttta tcggtcactg cttgggtccc cacgcgttac
     8521 ttgcggtaga aaaataaaat tcggcgcaat tctaacaggg aaagcaaacg tttgcgagac
     8581 tgctttacac aacctttttg cacgtctttt ccccaggcgc gcggcgaaag aagacttgtg
     8641 ccagggcaaa ggttagtttt cggatggaat aatcttcttt cataaccatt tgaatataaa
     8701 ataactttat ctcaaaccgt tatcattttg actaaagtca acgaaaataa tattgccgcc
     8761 ttgaagaaag gaggtataat ccgtcgattt tttttgtggc tgcccctcaa aggagaaaga
     8821 gtatgaagat cgtggaagtc aaacacccac tcgtcaaaca caagctggga ctgatgcgtg
     8881 agcaagatat cagcaccaag cgctttcgcg aactcgcttc cgaagtgggt agcctgctga
     8941 cttacgaagc gaccgccgac ctcgaaacgg aaaaagtaac tatcgaaggc tggaacggcc
     9001 cggtagaaat cgaccagatc aaaggtaaga aaattaccgt tgtgccaatt ctgcgtgcgg
     9061 gtcttggtat gatggacggt gtgctggaaa acgttccgag cgcgcgcatc agcgttgtcg
     9121 gtatgtaccg taatgaagaa acgctggagc cggtaccgta cttccagaaa ctggtttcta
     9181 acatcgatga gcgtatggcg ctgatcgttg acccaatgct ggcaaccggt ggttccgtta
     9241 tcgcgaccat cgacctgctg aaaaaagcgg gctgcagcag catcaaagtt ctggtgctgg
     9301 tagctgcgcc agaaggtatc gctgcgctgg aaaaagcgca cccggacgtc gaactgtata
     9361 ccgcatcgat tgaccaggga ctgaacgagc acggatacat tattccgggc ctcggcgatg
     9421 ccggtgacaa aatctttggt acgaaataaa gaataaaaat aattaaagcc gactttaaga
     9481 gtcggctttt ttttgagtaa agcgcctata acacataata cagaggataa tactatgacg
     9541 cgccgtgcta tcggggtgag tgaaagaccg ccacttttac agacaatccc gcttagtttg
     9601 caacatttgt tcgccatgtt tggtgcaacc gtcctggtgc ccgtcttatt tcatattaac
     9661 ccggcgactg tactgttatt taacggtatt ggaacgctgc tgtatctctt catctgtaaa
     9721 gggaaaattc cggcttatct tggttccagc tttgccttta tttcaccggt attgttactg
     9781 ttgccgttag ggtatgaagt cgcgctgggc ggctttatta tgtgcggcgt gctgttctgc
     9841 ctggtttctt ttatcgtgaa gaaagcgggg accggctggc tggacgtgct gtttccacct
     9901 gcggcaatgg gcgcaatcgt tgccgtcatc ggtctggagc tggcgggcgt agctgccggt
     9961 atggcgggtt tactcccggc tgaagggcaa acgccagact ccaaaaccat catcatctct
    10021 attaccaccc tggcggtcac ggttttaggt tccgtgctgt tccgtggttt cctggcaatt
    10081 atcccgattt taattggcgt gctggtgggg tacgcgctct ctttcgcaat gggaattgtc
    10141 gataccacgc cgattattaa tgctcactgg tttgcgctgc caaccctcta tacgccgcgc
    10201 ttcgagtggt ttgccattct gactattctg ccagcggcgt tagtggttat tgccgaacac
    10261 gtagggcacc tggtagtaac ggctaatatc gtcaaaaaag atctgctgcg cgatccaggt
    10321 ctgcaccgtt cgatgtttgc taacggcctg tcgactgtga tctccggctt ctttggctct
    10381 acgccaaata ccacttacgg agaaaacatt ggcgtgatgg cgatcacccg tgtttacagt
    10441 acttgggtta tcggcggggc ggcgattttc gctatcctgc tttcctgcgt cggtaaactg
    10501 gctgccgcta tccagatgat cccattgccg gtgatgggcg gcgtttcgct gctgctttat
    10561 ggtgtcatcg gtgcttccgg tattcgtgtt ttgatcgaat cgaaagtgga ttacaacaaa
    10621 gcacagaacc tgatcctgac ttccgtgatt ttgatcatcg gcgtcagtgg cgcgaaggta
    10681 aacatcggcg cggcggagtt gaaaggtatg gcgctggcga ccatcgtcgg tatcggctta
    10741 agtttgatct tcaaactgat cagtgtgctg cgccctgaag aagtggtgct ggacgcagaa
    10801 gacgcggata taacagacaa ataatgtacc gaccgggcag tgttgctgcc cggttcaaac
    10861 atcatgggat tctgtggtaa acttctcgcg attttgtgaa atcctggttg aggtatctct
    10921 gaacacaccg gcacagctct ctttgccact ttatcttcct gacgacgaaa cctttgcaag
    10981 tttctggccg ggggataact cctctttact ggccgcgctg caaaacgtgc tgcgtcagga
    11041 acatagcggt tacatctatc tctgggcacg cgaaggcgcg gggcgcagtc atctgctgca
    11101 cgcggcttgc gcggaattgt cgcagcgtgg cgatgcggtg ggctatgtcc cgctggataa
    11161 acgcacctgg tttgttccgg aagtgctcga cggtatggag catttgtcgc tggtctgtat
    11221 cgacaacatt gagtgtattg caggcgatga gttgtgggag atggcgattt tcgatctcta
    11281 caatcgaatt ctggaatcgg gcaaaacacg gttgttgatc accggcgatc gtccaccgcg
    11341 gcagttgaat ctgggattac cggatctcgc gtcgcgactc gactgggggc agatctacaa
    11401 attgcagcca ctttctgatg aagataagtt gcaggcgcta cagttacgcg cgcgtttgcg
    11461 tggttttgaa ctgccggaag atgtggggcg tttcttgctg aagcggctcg acagagaaat
    11521 gcgcacgcta tttatgacgt tggatcagtt ggatcgtgcg tcgattaccg cgcaacgtaa
    11581 gctgaccatt ccgtttgtga aagaaattct gaagttgtag ataaggtgtt tattgtcgga
    11641 tgcggcgcga acgccttatc cgaactacgc gaagttaatc catagtattc gtaggccgga
    11701 taagacgtgg caagcgtcgc atccggcaat gtgcttcaac tagcgatgga gcacatctta
    11761 acccacgatt tccagtacct gctccggtgg acgaccaatc cgcgctttgc cattcgcgac
    11821 cacaatcggg cgctccatca gcttcggatt atccaccatt gcctgaatca gcgcctcttc
    11881 gctaagcgaa ctgtcagcca ggttcagttc tttataaaga tcctctttct ggcgcatcaa
    11941 ttcacgggcg ctattcatcc ccagtatttt cagcaaatcg cgcagcgttg ccgcatcggc
    12001 gggtgtctca aggtagagca ccacttctgg ctccacgccg ttttctttca gcaggttcag
    12061 cgtttcccgg ctctttgaac agcgtgggtt atggtagatt tttacctgtt tggtcattgt
    12121 tcttccttta atgcgaatta catcttggta taaggcttaa agcgttcctg cagctggcgc
    12181 aactggtcga tgcgcgcatc gtaacgcgct tgttgcaggc tgcctaattt cacctgcgaa
    12241 ctggcgctac tcaacagcga aatggcctga tcgagtcgtc cggcgagcgc ataaccttct
    12301 gctcgcgcag ccagctcctg atcgcggtta tttagcgcgg cttccgcctg tgccagcaaa
    12361 tcccagccgt tgctgtcatc tttattatta aaggtgtagc gattcagaat attggccgct
    12421 tcttgtggtt gaccgccttg caaataagcg ttcgccaggt tgagctgcaa caccggattg
    12481 gtgcgtaaat cgcgggcatt tttcagacga ttgatcgcct cattggcttt gttttgccca
    12541 agatcgatat cggtagccag atcgagatac catgcgttgc caggttctgc cgccagtaac
    12601 ggttgcagcg ttttacgcgc ctcgtcgtat ttattggctt ccatcgcctg taaagcacga
    12661 ccatattgtg ctgctcgttg ctgacgaacg tttcctttcg cccattcatc cagcaaatca
    12721 ctggtgagct ggttacgtcc ggaattatac atccccagtg tgcgcgcttt cgccagatag
    12781 aaatcttccg acgactgcac caccatcggg cgcatctgat tagcacggtt gcgggcatct
    12841 gccagacgac tttccggcaa cgggtgagtc aataaaattt ccggcgggcg cgaggagtaa
    12901 cgcgcctgat cgagtaattt ttcgaggaag gttggcatcg cctgcggatc gaatcccgag
    12961 cgttgcagca cctgaatacc aatgcggtcc gcttcctgtt cattttgctg ggtgaaactg
    13021 atcatcccct gacgtgttcc cgccagtgta ccggtcagcg ccgccatccc cgcctgcgga
    13081 ctggccatcg ccagtaaaat agaacctaac gcgccgaccc aggtcagcgg cgcgctgcgt
    13141 tgttgatctt ccatcgctcg cgccaggtga cgttgggtga cgtgggagat ttcgtgcgcc
    13201 ataactgaag ccagttgact ttcgttatcg gaataacgga acagggcaga gtgcagcacc
    13261 acgttgccgc caaagaaagc aaaggcgtta atttcgtcgt tgttgatcag aaaaaaatga
    13321 aacggtgtct taaccgaatt ggcatgcgaa accagacgca tccccagcga attaatatat
    13381 tgcgttaaca gcgggtcatt aattaacggc gcgctgccgc gtagctggcg gacataatag
    13441 tcgcccatct gcatttcctg accaatggaa agcgtgcttc ctgcggaggt tcccatatcc
    13501 ggcaaggtgt ctgcgctgtc ggcaaacgcc ggggctacct gaccaatggt catagcagca
    13561 atgagggttg caaccaggtt ttttttcaac tgcctgaaca taacctctat cctgtatttc
    13621 tgaagatagt catttgaccg atactgtgaa atatcgttcc caccgcgccg catttgagtg
    13681 taacggcgaa gattagccca ctcaaatgac ttttcagttt cctgaatccg tcataaaaag
    13741 cgaagtcttt tttgtgacat ttcgatacaa ttgcggatcg caatcccgct cttgaggttc
    13801 agggaaggtc tttatgctcg aaatgttgat gcaatggtat cgccgccgtt ttagcgaccc
    13861 ggaagcgatt gccttgctgg ttattttagt tgccggattt ggcattatct ttttctttag
    13921 tggcctgctt gctccgttgc tggtggctat tgtgctggcc tatttgctgg aatggccaac
    13981 tgtgcgcctg caatctattg gctgctcccg ccgctgggcg acgtcgattg tattggtggt
    14041 ttttgtcggt atattgctac tgatggcgtt cgtggtactg cctatcgcct ggcaacaggg
    14101 catctactta attcgcgata tgccggggat gctcaataag ctttctgact ttgccgccac
    14161 gttgccgcgc cgctatccgg cgttaatgga tgcgggcatt attgatgcaa tggccgaaaa
    14221 tatgcgcagt cggatgctga ccatgggcga ttcggtggtg aaaatttccc tcgcctcgct
    14281 ggtcggtttg ctgaccatag ccgtctatct ggtgctggtg ccattgatgg tcttcttcct
    14341 gctgaaagac aaagagcaga tgctgaacgc cgttcgtcgg gtgctgccgc gcaaccgtgg
    14401 actggcagga caggtgtgga aggagatgaa tcaacaaatc accaactata tccgcggcaa
    14461 agtgctggag atgatcgtgg tggggatcgc cacctggctg gggttcttgc tctttgggct
    14521 gaactattcg ctgctgctgg cggtgctggt cggcttctcg gtacttattc cgtacattgg
    14581 cgcatttgtg gtgaccattc cggtggttgg cgtggcgcta ttccagtttg gtgcaggcac
    14641 ggaattctgg agctgcttcg cagtgtatct gattattcag gcgctggacg gcaacctgtt
    14701 agtaccggtg ttgttctccg aagcggttaa cctgcatccg ctggtgatta ttttatcggt
    14761 ggtgatcttc ggtggtttgt ggggattctg gggcgtattc ttcgccattc cattggcgac
    14821 cctgatcaaa gccgtgatcc acgcctggcc cgatgggcaa atagcgcaag aataatcttc
    14881 agcccgacga actgtttcgc cgggctgatt tttcaggatt cctgacgtaa atagatagcc
    14941 cgataacaca tactcaccag cactgcaccg ccgataatat tacccagcat caccgggagc
    15001 agattggcgg taataaaatg gcttaccgtc agtgccggaa aattgtctgc gctactgtgc
    15061 gccagttgcc agaagggggg aggggcgaaa tggcgaatgg caatggcgaa cggaatcaca
    15121 aacaaattgg cgatacagtg ctcaaagcca ctggcgacaa acagggtgat gggcaaaatc
    15181 atggcgacga ttttgtcgca taacgaacgc ccgcaataac tcatccaaag cgccaggcaa
    15241 accatcagat tgcacataat gccgaggctg acagattcag taaatgtatg atgcattttg
    15301 ccctcggcgc agtgtaaaac cgcgactccc cacatcgcgt tttcactcat caccagcccg
    15361 gaaaaccaga ttaacaaact gaaacaggca atacctgcca gattaccgca ggccaccaga
    15421 agtgcgttaa tcagccaagt tcgccaacta ataacgcccc gactttttgc catcaccgtc
    15481 attaccgacg aggtgaacag gctggtgccg caaaccgcca gcaaaataaa gccgagtgta
    15541 aagcaaaggc cgcccaccag atgggttaat gcctgtgaag acggggcatc ggcgataacg
    15601 gaaaggtaaa aagtaaatcc gatcgccata aacacgccag cggacatcgc cattaaaaag
    15661 accgacactt tactgcgggc aattttatgg gcggcaatcc gttcagcgat tgccgctttt
    15721 ctggcgctca actgcaagtc gaaagagagt ttgtttctca tgcatactcc tgcaaaagca
    15781 gattacaaca cctcgcgaac cgagatcccc agacgctgca ttcgtgacag cagcgtggtg
    15841 cgcttcatcc ctaatcgtgt cgccgcgcca cggggcccgg caacaatgcc attggtttct
    15901 cgcaatacct gaacaatgcg ctgacgttct tcttcgtcat tctctggcgt cgtcgggtgc
    15961 agcaactgcg ccattgaact gcgaagcgct gaatcttcat ttagcgtcgg cagtaaacgg
    16021 ctttgtcgga catttagatg taaattcaga ctgttaccac gagtcaacag taccgcccgc
    16081 tcaatcacgt tttccagctc gcgcacgttg cccggccaat cccacgacat caactggcgt
    16141 agtgcctcgg tcgggatggc gtcaattgcg cgattcatat ggcgcgccat tttttgcgtg
    16201 aaatgctttg ctaaaagagg gatatcttcc ggacggtcgc gcagcggcgg caattccagt
    16261 gggaagacat tcaggcgata aaagagatcg ctgcgaaact ggcgatcttc aaccatttgc
    16321 cacaaatcac ggttggtggc ggcaatgact ctgacattta ccgggatcgt tctactcccg
    16381 ccgagacgct caatctcccg ttcctgcaat acgcgcagca gtttaggctg aagttctaac
    16441 ggcagatcgc caatttcatc gagaaacaac gtgccgccat cggcaatttc aaaacgacca
    16501 cgatgggtat taatcgcacc agtaaacgcc cctttgtcat gaccgaataa ctcactttcc
    16561 agaagactgg cggggatggc agcgcagttg attttgacca gcggcttgtc gcgtcgcggg
    16621 ctaagttgat ggatcgctct ggcgatcacc tctttgccgg ttccggtttc accgcaaatc
    16681 aacaccgtac tgtcgctctt cgccacaata tctacctgct ggagtaggtc ttccattgcc
    16741 tggctttgat agataatgtc accgatgccc agattcgaaa ggagctgctc gctaagctgg
    16801 tggttttctt gctgcaaact ttcctgcaaa tcggtcatgc tacgccaggc atcggcattg
    16861 ccaacggcaa tagcgatgcg atcggctatg tgttgtagta gctggcagtt ttcctcacta
    16921 aagagagtgg aagaggtatg cgccagcaac aatgcacccg gtgtatggtt gccaaaggta
    16981 agcggtataa ggagcgcaga ttcgcagccg ttagatgcaa gtaagagtaa taacgggtcg
    17041 cgttgccaga gaaacagatc gtctgcacgg tgcgtcaagg tcggttgtcg ggtttgtagc
    17101 gtttgtgtca gcaatacact gtcgccaggc atattgcgtg gcagacacgc acaatgtgag
    17161 gcagaaagat cgctgcacca caggctaaac ttctcgttct ttcgatgatc gcccagtacc
    17221 atactgactg aagccagacc gaaaaaatga tggatctcac gagcgacgtc agcgatcaga
    17281 tcgtcgagat caagatgtga cagcactgca ttagtaatat caaccagtac ccgatgatga
    17341 tcgcgatctt tctccagcaa acgcagcgcc acctgctgtt cttcatcatt gagccatgat
    17401 tgcagaaccg gggtgattat ccgcgccagc cagttaaaca gtgtgcaatc ctggggagta
    17461 aaacgactgc cgtgggtgcg aataaagcgt agctccgcca ttccactact gccgagaagg
    17521 acggcgtgac gcgaataatc gaccatctcg ccctgatact ctgttgttgc cgtcacatcg
    17581 ctgccatcat catggtagcg gtaatagaag ttgcttttga gaacatgcag ttcaataagt
    17641 tgtaccgggc tgaacggcgg cggcagcggg atgaaggcgc gtaataaaga cgccttaccg
    17701 tgtttctgcg ctaaccgctc cgcgagcatt ccgtttaccg cttcaattgt tattccttgc
    17761 ggcggggcaa acatcgcctc gtctgacata gccatcaaca gtccttatta atgagaaatc
    17821 cttctcacgt aatttttctg gccatcagat agatggccgg ttcctgcaca atttcatcca
    17881 acagatgaat gaattgaatg ctacgcgcct gccacacaga cgtcgcgtca tccgccagtg
    17941 gcgcgaatgc ccgggtcaac agggcggtgt ggctggcgtc aataacaatc tcaccaaaag
    18001 tcatcacccc cagcatctta cgtcgggctt gctcctcttc gaccagtgca agccaatctt
    18061 cgtattccgt cagtgggcag cggaaggcga cattcagaca atcaatcacg ccaacgtgat
    18121 ggccgatagc tagcgagtaa tacattacct gagagctgtg ttccggcatc tcgtcgctac
    18181 tggcgacaaa cttttttcgc agcgtccaga aaacaatttc cccgcactct tcagtcatgt
    18241 aacccccgga gtacgcgaaa gagttgctga acgatactat tgagacgtgg atcgtcggca
    18301 tcgcgcaacc aggtgttaac ccgatttcct gtaggatcgt cgcttaaatg gcgcaggagc
    18361 cgatcgcaaa tttctcgccc ctgacgatag cccgccagcc gccgcgcttc tcgctcaatg
    18421 gcgatacgct gtgatggcgg gatctgcggc cacaacggtt gcatagtcac cccggtggga
    18481 tcgcgataat ccacagcgtg aatcttctgt tgcagcaaac cgagcgccac ggcgaaaccg
    18541 tgaatggtgg cggccggtgt tggcgggcag ccggggatcc aaacatcaat ggggacaatg
    18601 gtgtcgctac cgccccagac gctgtagaga tcgtggaaaa taccgccgcc gacaccgcac
    18661 gcgccgtagg aaacacaaat tttatgatcg ggggcagact catacgcccg aagtgcaggc
    18721 atacgcatcg cccgggtgac tgcgccagta aataacaaaa tatcggcgtg acgcggtgat
    18781 gaaacaacct taatgccaaa acgttctgcg tcgaatactg gtgtaatggc agcaaaaatt
    18841 tcgatttcac aggcgttgca gccgccgcaa tcgacgcgat aaacgtaagc cgagcgacgg
    18901 atatcctgta gcaaatgccg cttcatcttt tgcgtttgct cgtccagcgt gatgggctgg
    18961 ctgacatgtt gtgtaagcac tggactcata gctgctcctt agccaccagc agtacatctg
    19021 tatcgtcgtt gatcagcgtc gcgcgttgtt tgcattccgg gcagacgctc gcttgcgccc
    19081 acaacatttc gcggttttgt ggcgcatttt gttgctgtgc taacaattca gcagccagtg
    19141 cgatggtttt ttgcggggca aacgggcgtt cgcaacggct gcaacgttgt agatggaacg
    19201 tcgcgcgggt atagagatcg gctttattgg tgacggtcag ttcaaagtta ttggtaagct
    19261 ggatggctct ggtcgggcac acttcttcac aacgtccgca gtaaatacaa cgccccagat
    19321 agagctgcca ggtgcgcgaa ttttgctggt cgtcggtctg gatagtcagc gcatttgccg
    19381 gacaagcaca ggcgcaggca ccgcaggcaa tacattggct gggcatcagg tccggttttc
    19441 cgcgaaagcc agggctgacc tccagtggcg cgaagggata tttcaccgtc gcggttccgg
    19501 cgcgcataat agttttcagt aacttcagca tcttctgtcc ttacttcagc ggcgaacggt
    19561 tacgatcaat gccgtagcgt tcgatctctt tatacggcac ggtttttgac tggcgcttgc
    19621 gcacatctac cagcgtcaca cggtcggtac aggagtagca gggatcaagg ctaccgataa
    19681 tcagcggtgc gtcagaaacg gtattgccgc gcagcatgta acgcaacacc ggccagttgg
    19741 cgtaggtggc ggcacggcag cgccagcgga acaatttttg gttatcaccg agcatgctcc
    19801 agtgcacgtc ttcaccgcgt ggcgcttcaa caaagcccag cgcgaatgcg tgaggtttat
    19861 agctaaagcc ttcggtcagc agtggggtat ccggcatgtt gtcgagggca aattccagca
    19921 ttgccagcga atcaaacgtc tctttgacac ggaccatcac gcgggagaaa acatcgccgc
    19981 cggtaaaggt aaacagtgtt tttggaatat tgccgtagtc ggcgtagggg tgatcaaagc
    20041 gcaaatcacg ggcaaaacca ctgccgcgga tcagcggccc tacagggcta tattcacggg
    20101 cgatttgtcg gtcgagaatg ccaatgccct gagtgcgttg ttccatattc ggcgtagcaa
    20161 gcagcatctc taccagctcc gacacgtcgg cgcgcatctc gcgcaccagt ttcagcgttt
    20221 gcagacgttg ctctttgaga atatcgcggc gaacaccacc aatcagattc agaccgtagg
    20281 ttttacgcga cccgatcagc aattccgcca tcgtcatcga cttttcccgc acgcggaaaa
    20341 attgcataaa gccggtatca aaaccaacga aatggcagga gaggccaagg ttaagcaaat
    20401 gactgtgtag ccgttcgact tccagcagaa tcgagcgaat agtatgtgct cgttgcggca
    20461 cctcaatccc cagtgcattt tcaaccgaat tggtataggc cacactgtgg gcaaaaccgc
    20521 aaatcccaca cacgcggtcc gataagaagg tcacttcgtt gtagcccatc cgcgtttctg
    20581 ccagtttctc catgccgcga tggacataaa acaggcggta atcagcatcg acaatttgct
    20641 cgccatccac aaacaagcgg aagtgacccg gttcatcgga ggtgatatgc agcgggccga
    20701 cagggatcac ccgcgcatcg ctgttgccct cattgataaa cggatacgtt tcggaatcag
    20761 tcgtcggttc agggcgcagt cgataatcca tcgcatcttt gcgcagcgga tgcatatctt
    20821 ccggccagtc atcgggcaac accaggcgac gctgatccgg caggccaacc ggaatcagcc
    20881 cgtacatatc gcgaatttct cgctcgcccc agaccgcggc agggacgcgc ggtgtgactg
    20941 acggaaactc ccgactgtcg gcatcgacca gcgccttcac cacaatccag catttttcgg
    21001 ccccttccat tgaaagggca taataaaccg cgtaatgacc gttaagtgtc cgctcgtcgt
    21061 tgccaaacag gaccggaagc cagccatcat gttgataata aagatactgt acaacgtcag
    21121 gcagcagatt gattttcacc gtaatggtga cctgttcagg cgtttgtcgc tcttcatcca
    21181 gcaccgcgcc ggggaactgc gttttcaggg cggcgagaat cgcttcgcca cgatttgacg
    21241 atgaattaac gttcactgtg tttctccgaa acagatgcgg tgcctgaagg caaaaaatca
    21301 tgccaggtgc tacgttgtgc aggcagatcg tgcgtccctg agaggactat agtggaagcg
    21361 cccgccagga tcctgatgac aggttgtgga atatgcgttc ccatcgccag catcatgacc
    21421 agcagaatca ccattggcga ggtggtcaac cagccgagat caccccggtt aacggcctgc
    21481 ggcggtttcg ccattaacac ccgcgcagcc atccgtacca ggcccgccag caccagcgtt
    21541 aacagcaata acagcaggac gataatcagc aggtgattac gtgccagtcc ggcggtaatg
    21601 gtcataaatt cgctaagaaa aatgttgaag ggcggcatcc ctgccagcgc catttaccgc
    21661 cgccaaacag cacggcggta aatggcatga ttttgagcat cccacagacg acgttgagat
    21721 cgcgcgtgcc gtacttgagc agtacattgc cggaaccgca gaacagcagc gtttttgcca
    21781 gactgtggtt taagatgtgc agcagcgcgg caaaaattcc cagcggcccg ccaatgccta
    21841 gctccaccgc gaccagcccc atgttctcca cgctggagta cgccagcaga cgcttaatgt
    21901 cccgctgtac cagaatgaaa aatgccgcca cggcaaccga caacatgccg aagatgagca
    21961 acaaccggtt ggggaaatcg ctgccgatgg cttggcaaat aatgatgtaa tagcgaatca
    22021 gcaccaacag cgcgcagttc agcaatacgg cggagagcag ggcgctgacc ggactcggcg
    22081 cttcactgtg agcatccggc agccaggcgt gcatgggaaa tagcccggtt ttggtaccaa
    22141 agccaattag caaaaacaca aaggccaaca gcattaatgt tgggtcaagc aaggacgatt
    22201 gcttaagaac ctcgctccag aatatcgcca tttctgcctg cggcataacg ctggcggcgt
    22261 tggcgtatac cagcacggta ccgaacagac caaaagcgac accaacagta caaataatga
    22321 tgtacttcca tgcagcttcc agcgatgaac gctgaccgta aatgcctacc agaaacgccg
    22381 agcttaaggt ggtggcttcg atcgccgccc acatcacaat caggttattg ctggtaacaa
    22441 ccagcagcat ggtgaacaaa aacagatgga agaaaccgta gtaatcgcac agcgtaacgg
    22501 gtgaaagctc gccgtgtgcc acttcatgac gcatgtagcc aatcgagtaa ataccggtga
    22561 gaaagccaat cacaccaaga atggcgagga acaaaccgcc cagaccatca atatgaagcc
    22621 acagtcccgc agcgaatatt tctcctgcat cagcggcagt ttggaccacc cagagtgcca
    22681 gaatcagcag cagtgtgatc cctaagctat gtaatacggt caccgtgcga gtcgcagaaa
    22741 gtctccgttt ccggcaggca aaacagagca gcgaaaaaag cagcggcgtg agcaggagta
    22801 aagcgaacat cacagaataa ctcatctcat tatcccttca gcgcggtcaa gttgttcacg
    22861 tccagcgtgc cgtgggtacg ccatattttt cttgccagta acaccatcac aatgacggcg
    22921 aagatggcgt cggtagcgat acctatttcc accagttccg gtgctcgcca ggcaagaagc
    22981 gccagcacca gatgggagcc gttttccatc aggcagtaac caaaaatttg ccgcaggata
    23041 ttgcgctggc tgacaatgca cagcaggcca agcagaaaat gacctaacgc taccgccagc
    23101 gccggtttca gcccggtagc catcggtagc ttcacgggct gaacgacaaa tgcgcaaagc
    23161 aggacaatta acgccgcgag cagtgccatc attgccggac cgaataacgc tttttccggg
    23221 atgttctggg gaatatttcg tgcagcgtaa gtcatgatta acggtaccag cagcactttg
    23281 gtgataaagg cgctggcgga ccagatcagc agttgctctg cggcgaacag gcacgagaga
    23341 gtggcgaaaa tagacaccag caccagtgac tggcaggcgt aaaatccgca tgacaggcga
    23401 tagcttttga cgctaatcac aaacagcgat gtcagcatca tcagtcccgc cagattattt
    23461 acgatcatag aaccggtcat tcatcatctc cttactgcaa ccaggtcgcg gcgattacgc
    23521 tggaaatcag cgacatcaca atcagtacaa tcaacaccag gcgcattgat cctggcagcg
    23581 gtgcggcatc ggcgacggcc tcgctcggtt tgccaggcac aacgcgacca aaccagcgaa
    23641 taaaccaggc gaaactggcg accgattcaa tcatcagaag aatcatggcg ggcagcagga
    23701 tccagtactc cactgacaac gcaaaaccgg cagcaaacag cgggaattta ctgaagaagc
    23761 cgttgaacgg cggcacgccg gtaatcgcca gcgctgccac gcagaaaccc acgcctggca
    23821 acggcagggt gtgcaatacg ccacgcagac gcggcaacaa gcgcgtgccg cagctgtaac
    23881 tcagcgcacc tgctacaagg aaaaacaggc ttttagcgaa cgcgtggttg acgatgtagg
    23941 cgatgctgcc ctccagcgcc agccgcgagc cgaagatgga gagcgacaag ccgaagaaca
    24001 tccagccaag ttgagtgatg gtcgaccagg ctagcaaccg cttcatatcc tgctgtggca
    24061 aatacatcag aaagccataa agaatggtga ccagtgccat gaccatgcca acgccgccaa
    24121 tcacatgcgg gatattgccg ccgtcgataa tagcgcgggc aaaaatgtaa acgcccactt
    24181 tcaccatcga tgcggcgtgg agataggcgc tgatcggtgt tggcgcttcc attgcgtccg
    24241 gtagccacgc ttgcatcggt agctgggccg atttccccca cgcggcaaac aggatgccgc
    24301 cataaaccag ataacgtgcg tcgccgtgta actcgctcat cgcgctaagc gcaaacgttc
    24361 cggtctgcaa aaacagcgtg gcggcggcaa gatacaaccc caacgagccg atatgagtga
    24421 taagtaacgc ttttagtgct gaacgctgcg ctttatcgct ctggtaataa ctgatcaacg
    24481 cccaggagca gccgcctgta atttcaaaaa acaacaactg accgagcagc gtcgaggaga
    24541 gtaccagtcc cgccatcgcg ccgataaaca ccagtaaaaa tgcgtaataa cgattcgtgc
    24601 cgttatgcgg gtgttcgcga tttttatccg tcagataacc cgtggagtag atcgtgacca
    24661 gcaaaccgag aaacaccacc acaaacagaa tcagcgtact cacgcgatca atgaccaggc
    24721 caaacaacgc cacatccccc acgttgacca acgtcaacgt gacggcaact ttatcggcct
    24781 gataaaacgc ggagatcagt gacaacatgc acagcgtggt cagcgcggcg aacaaaaccc
    24841 cccattcggc ggcccgacgt tgtggcgaaa acgaaacgac cagtgcgccg ataaaaggca
    24901 gcaataacgt cgtcagagca agattttcca tattccgtca gtgctcctta cagaccggtt
    24961 aaccagaaga cccatgcaag cgcagcaagg ctgaagccaa gccaggtcac atggtgaatg
    25021 agtaaaaaac gcccgcgtgc cagcgtgttt tctgcgattg aggccagtac aaaaatcagc
    25081 aaaaccttga gcagcgtaac gacaagtgaa gtcagcaggc aggcgagaga aagttcttgc
    25141 gcgcgcccaa agggcagaaa cagggccaca aacagtgctg ccatcacgac ctgtttcagc
    25201 cccagccccc atttcgctag cgccagcccg gcaccggaat attcggtcag cgggccttcc
    25261 tgtaattcct gttctgcttc agcaacatca aagggaattt ttcccatctc aatgaagcag
    25321 gcaaaaccac aggccagtaa cgccagtacg gtggttagcg gcgagttcca gcccatcgcc
    25381 agcgtattgc tgatcatctc gatatgcgtg gaacctgcta tcagcgccaa taccagcagt
    25441 gagagaataa gcattggttc gaccagaatg ccgagcgtca actcgcgact ggcaccgact
    25501 cccgcaaacg gacttccggt atccagcccg gaaagagcaa agaaaaaacg aaacagggca
    25561 agaagataga taagggtgat cagatcgccg ccgcccgcaa aaggggaaac ggtaataaac
    25621 agtggtaagg ccatcgccag caccagcatg ctgctgatta atacccacgg catcaggcgg
    25681 aacatcagac ctgaagatgt cggcgcaact tcctggcgtt taaacagttt gtggatatcg
    25741 cgataatcct gccagatccc cggcccgcgg cgggagtgca tacgcgcgcg tatctgccgg
    25801 gaaatacccg taaaaagtgg ggttagcatc agcagaatca cggcctgtgc taacgcaaaa
    25861 atgaccagat atccgtcgca aagagtttgt ctcatggtga tttccttaga cggcaatagc
    25921 gattagcagc acaaccagtg cggcgaccac atacaggcaa tagagacgaa agtcgccgct
    25981 ttgcagatgc tgtatttctt tggccagccg ttgggtggcg ctcacgatgg ggcggatcac
    26041 ccgctcatcc cagaagggtt ctgtgctctg agccctggcg gtgacgtgcg caagaccttt
    26101 atttagcctc agcgtagggt cgagctgttg tcgtagacga aatagcgcag aaaagaccac
    26161 acgcagcggc tgcatcacgc cattgcctga cggggccatc gcattttccc agccgtagcc
    26221 gcatgcccag gcatctcctg tgcgacgaaa cgctgcgcgg cgcgaacgac accacagcca
    26281 gtaaatgcca ggcagcaaag gcatcgccag tagtaacagc aacagtaatg agggggtgac
    26341 ctgtgtatga aacgtgccgg gtacaagtgc tattccgctg gcgacagtgg cgggaggggt
    26401 attggttaac gcatgggcaa tatgcattat cttcggtgcc agccaactgg cactaagcgc
    26461 aattaatacg cagagtgccg cgagcagtag catggcgacg atcatcgtat ttggcacttc
    26521 ctgtgcctct tcagcgtgtg tactgcgcgg cgcaccacag aaagtaatac cgtacatttt
    26581 tacgaagcac attactgcca gcccaccagt gactgccagc attaccatag caataggacc
    26641 cgcaagttgt agcgctacgg cttccacacg acttagtgag aacagcgact gccaggtgta
    26701 ccattcgctg ataaaaccat tcagcggagg aatggctgat atcgcgaggc aaccaatcag
    26761 gcatgctgcg gctgtccacg gcatccgttt cgctagtgcc cccatttttt ccatgtcgtg
    26821 ggtatgcaaa cgcgaaataa tcgctcccgc gccgagaaat agcagccctt tgaacagcgc
    26881 atggttgagc agatgaaaca gtgcgccgag cagtccaacc acggtgagca gcgggtcgtg
    26941 cagtgacaga ccgaccatcg ccacaccgac tgccagcaaa ataatgccga cgttttcgac
    27001 ggtactccag gccagcagcc gtttgatatc ctgttccgcc agcgcatata gcacgcccag
    27061 gagcgcggag attgcgccga tcgccatcac cagaatgccc caccacagag gcaaacccgt
    27121 ttgcgccagc agatccatcg ctactttcag gatgccgaaa atacctattt tgaccattac
    27181 gccagacatc aacgccgaag cgtgcgatgg tgcggcaggg tgagcgcgcg gcaaccagct
    27241 gtgcaacggt atcatcccgg ctttcgcgcc aaaaccgaaa aaggccagca gaaacaccgc
    27301 cgacgccagc cccggagaaa gtgaaagcgt gcgaaactgg caaaatcgag gctgccgctt
    27361 tcgcgcccca tcagcaagaa ggcgatcata atcagcaccg atccggcgtg ggcgataaaa
    27421 aagtagagca tgccagcgtt gatcgacgtt ttatcctgcc tggcaatgac cagaaaccag
    27481 gaagacagcg acatcatttc aaacagcacg atgaaccaaa aagcgttgtc catcaccagc
    27541 agggcaacca tcgatgcgat gaaaagattc ataaagaagc ccatcgccgc cgcgcctttg
    27601 ccctcgtatt cgcgcatata agtcaatgaa tagagcgaac aaaccaccac cagcaaggag
    27661 ataaccagca ccataaatgc cgagaggcta tccattcgca gggttaaatc ggcaaagtca
    27721 aaaggggtgg caaaatgggc gactaaagtt acaccagcat gtaattgcgt aattccgctg
    27781 atgatgccaa tcacgccgcc caccagcgat gtgatgccgg aaagcttaat agccagtctg
    27841 tccagaccga gtaaaaacag cgaagccaga ctggcaaaca gatagaggat cagcgaccag
    27901 gttaataatt gcagggcatc cattagcgtt gctcctgagt gagggaaagg ggatcctccc
    27961 cgccattggc aaaccagctt gcggcaaggc gctgtttttc tttcatctga cgttgcaggc
    28021 tatcgtcggt gatcagccgt aacgcctgat tcgggcaagc gcgaacgcag gccggacctt
    28081 ctggcaagaa atcacacagg tcgcatttca ccgcgacggt ctggacacca ggttcccagc
    28141 gcagcaacgc atgttggggc agcacgtttt cttcgccgtt tttgagtgag ccttcagcct
    28201 gaaaaacata ttgcgcatgg gcattcaccg gacggcttcc tgaagcgctg attgcgccaa
    28261 atgggcagac cacggcgcaa agcttgcagc caatacagag gctttcgttg agttggatcg
    28321 catcatccct ctgagagatg gcattgaccg ggcagacctg caggcaaggg gcttcctcac
    28381 agtgatgaca cacgacaggg gcagtgattg ttgacgtctt cgccagggcc aggcgcgggt
    28441 gttgctgtaa accttgcgtt ttatgcacgt ccgaacaggc agcgagacag gtattacatc
    28501 ctgtacacca cagtggttcg gccaccacaa agcggttcat gtcggctcct tgaaaatgat
    28561 atgaaacatt gcatttcata ttgcataaag gatgccattc tgattaactc aatatattca
    28621 ataagttatg tattaactgg attctgaacg gtacaaaaat gatggtcatt aacgttcagc
    28681 aaaaaatgga ttcgacaccc gtgtcgcagg ggaaagcgag aatgtgatct tcatcacgag
    28741 aagtgaggta tgctggccgc aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt
    28801 gttctttcag ccagttcagc acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga
    28861 catgttcgat tttgccatca gcgtcaatca ggaagctgat gcgatgaatg ccatcgtagg
    28921 ttttgcccat gaaggacttt tcaccccaga cgccgaattg ttcgcacacc tggtggtcct
    28981 catcagacag gagcgtaaag ttaagcagct ctttttccgc aaaacgggag agtttttcgg
    29041 gtttatcggt gctgataccc agcacatcaa cgcccgcttt tttcaactca tccatgttat
    29101 cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat ggctttcggg tagaaataaa
    29161 ccagaacacg ctgtccctgg aagtcggtca aattaacttg ttctccgtct tgatccggca
    29221 agctaaattt cggtgcgata tcaccggctt tcagtggatt cattacttaa ctccatcctg
    29281 ttcatcatgt tgggaataat tgacgacgtt aatactgcct tgtgcattga gttctgtaca
    29341 tagggctttg aacgcttgct caatatttgc tgcgtccgca gatgcggggc tgtgggcggt
    29401 tatctgaata tgcaactgcg cagcccgttc attttcagca ggttgcgtgc gcgacaccag
    29461 ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg
    29521 ggagtctgcc acatcgacct gaacccagac agatgctggc attggcggac gcggacgcgc
    29581 cgtcgtgcgc ttcatcacga ttaaaagatc cagttcggca cctttcaacg gtaacgttga
    29641 ttcaatcaga gtaatggcat tccatgaacc ggaaagcagc ataataaacg tgaactcttc
    29701 tcccagcatc gccaggcgac tgtcttcaat attacagccg caactactga catgacgggt
    29761 gatggtgttc acaattccag ggcgatcggc acccaacgca gtgatcacca gataatgttg
    29821 cgatgacagt gtcaaactgg ttattccttt aaggggtgag ttgttcttaa ggaaagcata
    29881 aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc ttgcttttaa tgccatacca
    29941 aacgtaccat tgagacactt gtttgcacag aggatggccc atgttcacgg gaagtattgt
    30001 cgcgattgtt actccgatgg atgaaaaagg taatgtctgt cgggctagct tgaaaaaact
    30061 gattgattat catgtcgcca gcggtacttc ggcgatcgtt tctgttggca ccactggcga
    30121 gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg atgatgacgc tggagctggc
    30181 tgatgggcgc attccggtaa ttgccgggac cggcgctaac gctactgcgg aagccattag
    30241 cctgacgcag cgcttcaatg acagtggtat cgtcggctgc ctgacggtaa ccccttacta
    30301 caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa gccatcgctg agcatactga
    30361 cctgccgcaa attctgtata atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac
    30421 ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc aaagaggcaa cagggaactt
    30481 aacgcgtgta aaccagatca aagagctggt ttcagatgat tttgttctgc tgagcggcga
    30541 tgatgcgagc gcgctggact tcatgcaatt gggcggtcat ggggttattt ccgttacggc
    30601 taacgtcgca gcgcgtgata tggcccagat gtgcaaactg gcagcagaag ggcattttgc
    30661 cgaggcacgc gttattaatc agcgtctgat gccattacac aacaaactat ttgtcgaacc
    30721 caatccaatc ccggtgaaat gggcatgtaa ggaactgggt cttgtggcga ccgatacgct
    30781 gcgcctgcca atgacaccaa tcaccgacag tggtcgtgag acggtcagag cggcgcttaa
    30841 gcatgccggt ttgctgtaaa gtttagggag atttgatggc ttactctgtt caaaagtcgc
    30901 gcctggcaaa ggttgcgggt gtttcgcttg ttttattact cgctgcctgt agttctgact
    30961 cacgctataa gcgtcaggtc agtggtgatg aagcctacct ggaagcggca ccgcttgcgg
    31021 agcttcatgc cccggctgga atgattttgc cggtgacctc cggtgattat gcaatcccgg
    31081 tgaccaacgg tagtggtgct gtcggtaagg cgctggacat tcgtccacca gcccagccgc
    31141 tggcactggt ttctggcgcg cgtacccagt tcacgggcga taccgcttca ttgctggtgg
    31201 aaaatggtcg tggcaatact ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact
    31261 acaccatcac ccaacgtgat gatgctggtc agacactgac caccgattgg gtacaatgga
    31321 accgtctgga cgaagacgag cagtatcgtg gtcgttatca aatctctgtt aagccgcagg
    31381 gttatcagca ggcggttacg gttaaactgc tgaacctgga acaggcgggc aaaccggttg
    31441 cagacgcggc ttccatgcag cgttacagca cggagatgat gaacgttatt tccgccggtc
    31501 tggataaatc tgccactgac gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg
    31561 acgtacaaag tgcagctgat gacaccggtt taccaatgct ggtcgtacgc gggccgttca
    31621 atgtggtttg gcaacgtctg ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca
    31681 gcacccgttc gcagggcaac atggccgtaa cttataagcc gctgtctgac agcgactggc
    31741 aggaactggg cgcgagcgat ccaggcctgg catccggtga ctataaactg caggttggcg
    31801 atttagataa ccgcagcagc ctacagttca tcgatccgaa aggtcatact ctgactcaga
    31861 gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg
    31921 ctggaatcat ccggcccttt tttctgatat gatacgcaaa cgtgtgcgtc tgcaggaaaa
    31981 cgcgatttta gcggtaattc gcacgaaatt tgtttgtcgg acgtagttcg gataaggcgt
    32041 tcacgccgca tccgacaaaa catccggcac accagacagc aaaagatttt aaaacgttaa
    32101 ttcacaccca ggagtgataa agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa
    32161 aaccgtatac agcacggaaa acccggacct gttggtgctc gaattccgca atgatacgtc
    32221 agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt
    32281 caactacttc attatgagca aactggctga agcgggtatc ccgactcaaa tggagcgtct
    32341 gctctccgat accgaatgtc tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt
    32401 gcgtaaccgt gctgctggct ctctggtgaa acgtcttgga atcgaagaag gtattgagct
    32461 gaacccgccg ctgttcgatc tgttcctgaa aaacgacgcc atgcacgatc cgatggtcaa
    32521 cgaatcttac tgcgaaacct ttggctgggt gagcaaagag aacctggcgc gtatgaaaga
    32581 gctgacctac aaagcgaacg acgtgctgaa aaaactgttc gatgatgctg gtctgattct
    32641 ggtcgacttc aagctggagt ttggtctgta caaaggtgaa gtggtactgg gcgatgagtt
    32701 ttccccggac ggcagccgcc tgtgggacaa agaaacgctg gagaaaatgg acaaagaccg
    32761 tttccgccag agcctcggtg gcctgatcga ggcctatgaa gccgtcgccc gccgcttggg
    32821 tgtacagctg gactgatttt tctgttcatc atcttgccgt gcagctggca cggcaagaca
    32881 accgctctcg gaatatatgc attgaacgct cattcctccg ccatttcatc ccgctttcct
    32941 gtggtaatct tcctctgaac cacctacgat catttctata atgaatatat tgttgaggta
    33001 tctatgcgtt ggcaagggcg acgtgaaagt gacaatgttg aagacaggcg caacagctcc
    33061 ggtggtccgt ctatgggcgg tcccggtttt cgcctgccaa gcggtaaagg cgggctgatt
    33121 ttactgttag tcgtgctggt tgcaggctac tatggtgttg atttaaccgg gttaatgact
    33181 ggacagccgg tttcccaaca acaatcaacg cggtccatta gccctaatga agacgaagct
    33241 gcaaaattca cctcggtgat tctggcaacc acggaagaca cctggggaca acagttcgag
    33301 aagatgggta agacctatca gcaaccgaag ctggtcatgt accgtggaat gacgcgcacc
    33361 ggctgcgggg ctggtcagtc cattatgggg ccgttctatt gcccggcgga tggcacagtt
    33421 tatatcgatc tctccttcta tgatgacatg aaagacaaac ttggcgcgga tggcgatttt
    33481 gcccaggggt acgttatcgc ccatgaagtc ggtcatcatg tgcagaaact gttaggcatc
    33541 gagccgaaag ttcgtcaact gcaacaaaac gcgacgcagg cggaagtgaa ccgcttatct
    33601 gtgcgtatgg aactccaggc cgactgtttt gccggtgtct gggggcatag tatgcagcag
    33661 caaggcgttc tggaaaccgg cgatctggaa gaggcgctga acgcggcgca ggccatcggc
    33721 gatgaccgtt tacaacagca aagtcagggg cgagtagtac cagatagttt cacacatggc
    33781 acttctcagc aacgctacag ctggtttaaa cgtggtttcg acagcggcga tccggcacaa
    33841 tgcaatactt ttggtaaaag catttaactt ccggggcagg gatggctgaa ctgactgcgc
    33901 ttcacacatt aacagcgcaa atgaaacgtg aagggatccg ccgcttgctg gtgttgagcg
    33961 gggaagaggg ttggtgtttt gatcatgcgc ttaagttgcg tgatgcctta cctggcgact
    34021 ggctgtggat ttcgccgcag ccagatgctg aaaaccactg ttcaccctcg gcgctacaaa
    34081 ctttacttgg gcgcgagttc cggcatgcgg tattcgacgc ccgccacggc tttgatgccg
    34141 ctgcctttgc agcacttagc ggaacgttga aagcgggaag ctggctggtt ttgttactcc
    34201 ctgtatggga agagtgggaa aaccaacctg atgccgactc gctgcgctgg agtgattgcc
    34261 ctgaccctat tgcgacgccg cattttgtcc agcatctcaa acgcgtactt acggcggata
    34321 acgacgctat cctctggcgg caaaaccagc cgttctcgtt ggcgcatttt actccccgta
    34381 ctgactggca ccccgcgacc ggcgcaccac aaccagaaca acagcaactc ttacagcagc
    34441 tactgaccat gcctccgggc gtggcagcgg taacggctgc gcgtgggcgc ggtaagtcgg
    34501 cgttggcagg gcaactcatt tctcgtattg cgggcagtgc gattgtcacc gcgcccgcaa
    34561 aagcggcaac ggatgtactg gcacaatttg cgggcgagaa gtttcgcttt attgcaccgg
    34621 atgccttgtt agccagcgat gagcaagccg actggctggt ggtcgacgaa gccgcagcca
    34681 tacctgcgcc gttgttgcat caactggtat cgcgttttcc tcgaacgttg ttaaccacta
    34741 cggtgcaggg ctacgaaggc accggacgtg gttttttgct gaaattttgc gctcgctttc
    34801 cgcatttaca ccgttttgaa ctgcaacagc cgatccgctg ggcacaggga tgcccgctgg
    34861 aaaaaatggt tagtgaggca ctggtttttg acgatgaaaa cttcacccat acaccacaag
    34921 gcaatatcgt catttccgca tttgaacaaa cgttatggcg aagcgagcca gaaacgccgt
    34981 taaaggttta tcagttattg tctggtgcgc actaccggac ttcgccgctg gatttacgcc
    35041 ggatgatgga tgcaccaggg caacattttt tacaggcggc tggcgaaaac gagattgccg
    35101 gagcgctgtg gctggtggat gaggggggat tatctcaaca actcagtcag gcggtatggg
    35161 caggttttcg tcgcccgcgg ggtaatctgg tggcccagtc gctggcggcg cacggtagca
    35221 atccactggc agcgacattg cgtggacggc gggtcagccg gatagcagtt catccggcgc
    35281 gtcagcggga aggcacaggg cagcaactta ttgctggtgc tttgcaatat acgcgtgacc
    35341 tcgactatct ttcggtgagt tttggttaca ccggggagtt atggcgtttc tggcaccgct
    35401 gcggttttgt gctggtgcgg atgggtaatc atcgtgaagc cagcagcggt tgctatacgg
    35461 cgatggcgct gttaccgatg agtaatgcgg gtaaacaact ggctgaacgt gagcattacc
    35521 gtttacgtcg cgatgcgcaa gctctcgcaa agtggaatgg cgaaacgctt cctgttgatc
    35581 cactaaacga tgccgtcctt tctgacgacg actggcttga actggccggt tttgctttcg
    35641 ctcatcgtcc gctattaaca tcgttaggtt gcttattgcg tctgctacaa accagtgaac
    35701 tggcattacc ggcgctgcgt gggcgtttac agaaaaacgt cagcgacgcg cagttatgta
    35761 ccacacttaa actttcaggc cgcaagatgt tactggtccg tcagcgggaa gaggccgcgc
    35821 aggcgctgtt cgcacttaat gatgttcgca ctgagcgtct gcgcgatcgc ataacgcaat
    35881 ggcaattttt tcactgactc cttcaatttt ctcccatggt cattgtgagt cagcggcgta
    35941 aaattaatga catcagttaa ggagttcgcc atgaaacatg accattttgt tgttcaaagc
    36001 ccggataaac ctgcgcaaca gttgttactg cttttccacg gtgtcgggga taacccggtg
    36061 gcaatggggg aaataggtag ctggtttgca ccgctgtttc ctgatgcgct ggtggtgagt
    36121 gtcggtggcg cggagccgag cggtaatccg gcggggcgtc agtggttttc agtgcagggt
    36181 attacggaag ataatcgcca ggcgcgtgtg gatgccatca tgccgacgtt tattgagacg
    36241 gtgcgctact ggcagaaaca gagcggagta ggggctaacg ccacggcgtt aatcggtttc
    36301 tctcaaggcg cgattatggc gctggagagc attaaagccg aaccgggcct tgcttcacgc
    36361 gtcattgctt ttaacggacg ctatgcgagc ctgccggaaa ctgcgtctac agccacgaca
    36421 attcacctga ttcatggcgg tgaagatccg gttatcgatc tggcgcatgc ggttgcggcg
    36481 caggaagcgt taatcagtgc cggtggtgac gtgacgctgg atatcgtgga ggatttaggc
    36541 catgcgatcg acaatcgcag tatgcaattt gccctggatc acttgcgtta taccattccg
    36601 aaacattatt tcgacgaagc gctaagtggc ggtaagcctg gcgatgatga tgtgattgaa
    36661 atgatgtaat aatagccggg taggcctgat aagactgtat caggcatcat ggccggatgt
    36721 ggcgtgaacg ccttatccgg ccactcagct tacttcttcg gttgatcctt tttcggccag
    36781 tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg ggggaagctc cggcttgttg
    36841 gcgagaaatt tcttatggtc gacgcgcttg agatctttaa tcacgttcag caggacgcct
    36901 accaaaaaga caatcaccag aatccaccaa tatttagcca gccagtccat gcttatttcc
    36961 tcttgcagaa ccactcatca cgcgacgagc tgttccatga tacgttgata catacgggca
    37021 agtagctgca ggtcggcagc gttcacacat tcattaattt tatgaatagt ggcattgacc
    37081 ggcccgagtt ccaccacctg cgcccccatg cgggcaataa agcgcccgtc ggacgttccg
    37141 cctgtggtca gtagctgcgg tttaatttca ttatagtgct caaccgcgtt aacgaccgca
    37201 tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg aaagccacca gtccaccgta
    37261 taacgcagtt gatgtttttc aagcagggca agcacctgcg ctttgatcat ctcatcagtc
    37321 agttcggtgc tgaagcggaa gttaaactgc acaaacagtt cacccggaat aacgttgtta
    37381 ctgcccgttc ccgcctgaat attagcaatc tgcatactgg tcgccgggaa aaattcattg
    37441 ccctgatccc actcaatagc cactaattca ttaaggaaag gtgctgcgcg atgtactgga
    37501 ttgtcagcca gatgcgggta ggccacatgc ccctgaacgc catgaatggt aaggttgcag
    37561 gttaacgatc cgcgacgacc atttttcacc acatcaccaa ccacttcgat actcgacggt
    37621 tcgccgacca ggcagtaatc gagacgctca ttacgcgaca ttaacgcttc gacgactttt
    37681 accgtaccgt tgtgggcact agcttcttca tcagaagtga tcagaaatgc cagtcgcccc
    37741 gtatggttgg gatgttgtgc gacaaaacgt tctgccgcta ccaccatcgc cgccagcgag
    37801 cctttcatat ctgccgcacc gcgcccgaat aacatgccgt cacgaatggt tggttcaaac
    37861 ggcggattga tccaacgatc ggcgtcgcca ggcggcacca cgtcggtatg cccggcaaag
    37921 gctaacgttt caccctgccc acgccatgcc caaaaattct gcgtatcggc aaagtccatg
    37981 cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca acaaagcctg gcatcctgcg
    38041 tcatcaggac tcagggaagg gcggcgaata agctgttgtg tcagctcaat aaccgggcac
    38101 gacatagact acacctcatg gaaaaattgc tgataactgg aatcactgaa acccagcagc
    38161 ataggcttac cgggcacgca gagcaatgga cgtttgataa ttgcaggcat ttcagtcatt
    38221 aatgccgccg cagaggccgc atcggtgatt ttattgcggg tggtttcgtc cagtttacgc
    38281 caggttgtac cacgggtgtt gagtaacgct tcccagccta attcgttgat aaaatcgttc
    38341 aataattcgc tgtccagccc atcgacgcgg taatcatgaa aacgatagtc gatgttattg
    38401 gcttccagcc aacggcgagc ctttttaatg gtgtcacaat ttttgatacc gtaaagtgta
    38461 accataatga atcctttttc gatttatgag aataaatact catttaaggg tgaaaacatt
    38521 ttgcctgc
//
LOCUS       DAZFZC010000040        38180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFZC010000040 DAZFZC010000000
VERSION     DAZFZC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.040"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(29..113)
                     /locus_tag="ACHKXP_002357"
     tRNA            complement(29..113)
                     /locus_tag="ACHKXP_002357"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77..79),aa:Tyr,seq:gta)
     gene            complement(148..279)
                     /locus_tag="ACHKXP_002358"
     ncRNA           complement(148..279)
                     /ncRNA_class="other"
                     /locus_tag="ACHKXP_002358"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(323..407)
                     /locus_tag="ACHKXP_002359"
     tRNA            complement(323..407)
                     /locus_tag="ACHKXP_002359"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(371..373),aa:Tyr,seq:gta)
     gene            complement(567..1409)
                     /gene="purU"
                     /locus_tag="ACHKXP_002360"
     CDS             complement(567..1409)
                     /gene="purU"
                     /locus_tag="ACHKXP_002360"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO7647345.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLTRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1459..1917)
                     /gene="ychJ"
                     /locus_tag="ACHKXP_002361"
     CDS             complement(1459..1917)
                     /gene="ychJ"
                     /locus_tag="ACHKXP_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO7647346.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            2030..2935
                     /gene="rssA"
                     /locus_tag="ACHKXP_002362"
     CDS             2030..2935
                     /gene="rssA"
                     /locus_tag="ACHKXP_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO7647347.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3027..4040
                     /gene="rssB"
                     /locus_tag="ACHKXP_002363"
     CDS             3027..4040
                     /gene="rssB"
                     /locus_tag="ACHKXP_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO7647348.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            4242..5150
                     /gene="galU"
                     /locus_tag="ACHKXP_002364"
     CDS             4242..5150
                     /gene="galU"
                     /locus_tag="ACHKXP_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO7647349.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5294..5707)
                     /gene="hns"
                     /locus_tag="ACHKXP_002365"
     CDS             complement(5294..5707)
                     /gene="hns"
                     /locus_tag="ACHKXP_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO7647350.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6312..6929
                     /gene="tdk"
                     /locus_tag="ACHKXP_002366"
     CDS             6312..6929
                     /gene="tdk"
                     /locus_tag="ACHKXP_002366"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO7647351.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVDSLTAIQERHRHD"
     gene            complement(7211..8107)
                     /locus_tag="ACHKXP_002367"
                     /pseudo
     CDS             complement(7211..8107)
                     /locus_tag="ACHKXP_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8231..10906)
                     /gene="adhE"
                     /locus_tag="ACHKXP_002368"
                     /gene_synonym="adhC"
     CDS             complement(8231..10906)
                     /gene="adhE"
                     /locus_tag="ACHKXP_002368"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO7647352.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            11299..11352
                     /gene="ychE"
                     /locus_tag="ACHKXP_002369"
     CDS             11299..11352
                     /gene="ychE"
                     /locus_tag="ACHKXP_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO7647353.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            11383..12030
                     /gene="ychE"
                     /locus_tag="ACHKXP_002370"
     CDS             11383..12030
                     /gene="ychE"
                     /locus_tag="ACHKXP_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO7647354.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSINYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(12188..12484)
                     /locus_tag="ACHKXP_002371"
     CDS             complement(12188..12484)
                     /locus_tag="ACHKXP_002371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647355.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            12768..14399
                     /gene="oppA"
                     /locus_tag="ACHKXP_002372"
     CDS             12768..14399
                     /gene="oppA"
                     /locus_tag="ACHKXP_002372"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO7647356.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPTGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            14485..15405
                     /gene="oppB"
                     /locus_tag="ACHKXP_002373"
     CDS             14485..15405
                     /gene="oppB"
                     /locus_tag="ACHKXP_002373"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO7647357.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            15420..16328
                     /gene="oppC"
                     /locus_tag="ACHKXP_002374"
     CDS             15420..16328
                     /gene="oppC"
                     /locus_tag="ACHKXP_002374"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO7647358.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            16340..17353
                     /gene="oppD"
                     /locus_tag="ACHKXP_002375"
     CDS             16340..17353
                     /gene="oppD"
                     /locus_tag="ACHKXP_002375"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO7647359.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            17350..18354
                     /gene="oppF"
                     /locus_tag="ACHKXP_002376"
     CDS             17350..18354
                     /gene="oppF"
                     /locus_tag="ACHKXP_002376"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO7647360.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(18407..18736)
                     /gene="yciU"
                     /locus_tag="ACHKXP_002377"
     CDS             complement(18407..18736)
                     /gene="yciU"
                     /locus_tag="ACHKXP_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO7647361.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(18771..20231)
                     /gene="cls"
                     /locus_tag="ACHKXP_002378"
     CDS             complement(18771..20231)
                     /gene="cls"
                     /locus_tag="ACHKXP_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO7647362.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            20374..20547
                     /gene="yciY"
                     /locus_tag="ACHKXP_002379"
     CDS             20374..20547
                     /gene="yciY"
                     /locus_tag="ACHKXP_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO7647363.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(20602..21855)
                     /gene="kch"
                     /locus_tag="ACHKXP_002380"
     CDS             complement(20602..21855)
                     /gene="kch"
                     /locus_tag="ACHKXP_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO7647364.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDELETKADSKESAQK"
     gene            complement(22155..22451)
                     /gene="yciI"
                     /locus_tag="ACHKXP_002381"
     CDS             complement(22155..22451)
                     /gene="yciI"
                     /locus_tag="ACHKXP_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO7647365.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            22675..23394
                     /gene="tonB"
                     /locus_tag="ACHKXP_002382"
     CDS             22675..23394
                     /gene="tonB"
                     /locus_tag="ACHKXP_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO7647366.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(23434..23832)
                     /gene="yciA"
                     /locus_tag="ACHKXP_002383"
     CDS             complement(23434..23832)
                     /gene="yciA"
                     /locus_tag="ACHKXP_002383"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO7647367.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(23937..24476)
                     /gene="yciB"
                     /locus_tag="ACHKXP_002384"
     CDS             complement(23937..24476)
                     /gene="yciB"
                     /locus_tag="ACHKXP_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO7647368.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(24506..25249)
                     /gene="yciC"
                     /locus_tag="ACHKXP_002385"
     CDS             complement(24506..25249)
                     /gene="yciC"
                     /locus_tag="ACHKXP_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO7647369.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSQEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(25277..25366)
                     /locus_tag="ACHKXP_002386"
                     /pseudo
     CDS             complement(25277..25366)
                     /locus_tag="ACHKXP_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            25606..26244
                     /gene="ompW"
                     /locus_tag="ACHKXP_002387"
     CDS             25606..26244
                     /gene="ompW"
                     /locus_tag="ACHKXP_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO7647370.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(26304..26810)
                     /gene="yciE"
                     /locus_tag="ACHKXP_002388"
     CDS             complement(26304..26810)
                     /gene="yciE"
                     /locus_tag="ACHKXP_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7647371.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQNIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(26856..27356)
                     /gene="yciF"
                     /locus_tag="ACHKXP_002389"
     CDS             complement(26856..27356)
                     /gene="yciF"
                     /locus_tag="ACHKXP_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7647372.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            complement(27442..27621)
                     /gene="yciG"
                     /locus_tag="ACHKXP_002390"
     CDS             complement(27442..27621)
                     /gene="yciG"
                     /locus_tag="ACHKXP_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7647373.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(28002..28808)
                     /gene="trpA"
                     /locus_tag="ACHKXP_002391"
     CDS             complement(28002..28808)
                     /gene="trpA"
                     /locus_tag="ACHKXP_002391"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO7647374.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(28808..30001)
                     /gene="trpB"
                     /locus_tag="ACHKXP_002392"
     CDS             complement(28808..30001)
                     /gene="trpB"
                     /locus_tag="ACHKXP_002392"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO7647375.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(30013..31374)
                     /gene="trpCF"
                     /locus_tag="ACHKXP_002393"
     CDS             complement(30013..31374)
                     /gene="trpCF"
                     /locus_tag="ACHKXP_002393"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO7647376.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(31375..32970)
                     /gene="trpD"
                     /locus_tag="ACHKXP_002394"
     CDS             complement(31375..32970)
                     /gene="trpD"
                     /locus_tag="ACHKXP_002394"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO7647377.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(32970..34532)
                     /gene="trpE"
                     /locus_tag="ACHKXP_002395"
     CDS             complement(32970..34532)
                     /gene="trpE"
                     /locus_tag="ACHKXP_002395"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO7647378.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(34554..34647)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(34624..34668)
                     /gene="trpL"
                     /locus_tag="ACHKXP_002396"
     CDS             complement(34624..34668)
                     /gene="trpL"
                     /locus_tag="ACHKXP_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO7647379.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            34806..35687
                     /gene="rnm"
                     /locus_tag="ACHKXP_002397"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             34806..35687
                     /gene="rnm"
                     /locus_tag="ACHKXP_002397"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO7647380.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            35684..36304
                     /gene="yciO"
                     /locus_tag="ACHKXP_002398"
     CDS             35684..36304
                     /gene="yciO"
                     /locus_tag="ACHKXP_002398"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO7647381.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            36332..>38180
                     /gene="yciQ"
                     /locus_tag="ACHKXP_002399"
     CDS             36332..>38180
                     /gene="yciQ"
                     /locus_tag="ACHKXP_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO7647382.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLQFSNIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNASAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRSDTFLQWGGLAILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFTAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQTHLSCGAYTSLLGRSYSG"
BASE COUNT         9708 a         9075 c         9516 g         9881 t
ORIGIN      
        1 tgggaagttc agggactttt gaaagtgatg gtggtggggg aaggattcga accttcgaag
       61 tcgatgacgg cagatttaca gtctgctccc tttggccgct cgggaacccc accaggggta
      121 attcaaattt cgaggtaatg cttgagatgg tggtggggga aggattattc gtcgcttcgc
      181 tcctcaccct tcgggccgtt gcctgtggca acgttctctc gctttcgctc gaatcgaacc
      241 ttagtcgaag gttctcaccc ttccccgatg agtgcaaact ttcacaatct caccgaagtt
      301 accacatcgc tgtggtgaat tatggtggtg ggggaaggat tcgaaccttc gaagtcgatg
      361 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccacg gggtaatgct
      421 ttttactggc ctgctccctt atcgggaagc ggggcgcatc atatcaaatg acgcgccgct
      481 gtaaagtgtt acgttgagaa aaatgaactg gttgcgtaat tttcatccgt aacggattaa
      541 aggtaaccag ttatttttgc tggcgattaa agaataatcg ttcgattacc gtaaacaaag
      601 acgcgctgtg ccagtacttt gtatagtgca cgacttaaga cgtttttctc gacgtcacga
      661 cctgcgcgca tcatatcttc agctgtgtag gtatgatcga catgaataac gtcctgcatg
      721 atgattgggc cttcgtccag attgtcattc acatagtgag cggttgcgcc aataatcttc
      781 acaccacgtt cataggcctg gtgataagga cgtgcgccaa taaacgctgg caggaatgaa
      841 tggtgaatat tgatgatctt attcgggaag cgtgccacaa attccggcgt taatacccgc
      901 atatacttcg ccagcaccac gtagtcaggt tgataagcat caatggcatc cgccatcttt
      961 tgatcgtgct cgttgcgggt taacccttca tggcttacca gctcaaacgg aatatcaaaa
     1021 cgctcaacca gagaacgtaa agtatcgtgg ttaccaataa ctgccgcgat ttcgacatcc
     1081 aggccgccgt aattggcttt catcaacaaa tcgccaaggc aatgcgcttc tttagtgacc
     1141 agaatcacta tccggcgacg accggcagga ttcagctcac gcacggagcc ttctggcaat
     1201 gcgctatcga gatccgccag cagggtggaa tcattaaaaa tcccttctag ttccgtgcgc
     1261 ataaaaaagc gcccggtacg gtgatcaaca aattcattgt tctgtacgat atttaactcg
     1321 tgcttgtagc aaatattggt aatacgtgcg atcagacctt tttggtccgg acaaatagta
     1381 cgcagaactt tacgttggag tgaatgcatt gctggaaaaa ccttgttgag agtgtttgct
     1441 aaaccgtacc gtcaaccatt attggccgca gcacttttta aattttttac ctgaaccaca
     1501 agggcaggga tcgttgcgac caaactgcgg acgtgtaccg tcaatatagt accattgacc
     1561 gttttctttc aaaaatcgcg aacgttcaat tattgcacct gttttaccac cttcggtaaa
     1621 tcgagcaaca aaactgacaa atccgatatt gtcggcgtcc tgccaacaat gttcaaatac
     1681 ggtcaatccc aaccattcag tatgcgcaaa tccggccatc aattcggcac gtaacgccgc
     1741 tgctccacaa gagggatgcc aggtctttat taaataatct gcgtcttgca tcacaaaagc
     1801 gcagtaacgc gaacgcatga gatgttctgg atcaggtgca accttttcac cagacacata
     1861 agggtggcaa cataggctat actcgacagc actaccacag ggacaaagct gagacacaaa
     1921 taatctccct ggaaacaata acggcgtatt aaccgcctga gtagcactat gttaaccgag
     1981 cagtagcgat gtggctacga ttgcattcca ggggaatctt gcgggaataa tgagaaagat
     2041 aaaaataggg ctggcgctgg gatctggcgc ggcgagaggt tggtcgcata ttggcgttat
     2101 taatgcgcta aaaaaagtgg gtattgaaat tgatatcgtt gcaggatgtt caattggttc
     2161 gctggtgggc gctgcctatg catgcgatcg attatctgcg ctggaagatt gggtgacctc
     2221 tttcagttat tgggatgttt tacgcctgat ggatctctcc tggcagcgcg gtgggttact
     2281 gcgcggcgag cgtgtcttca atcaataccg cgaaataatg ccggaaacag agatcgaaaa
     2341 ttgttcccgt cgctttgcgg ctgttgccac caatttaagt acgggacgtg aattatggtt
     2401 tactgaaggc gatctccatc ttgctattcg cgcatcatgc agtattccag gactcatggc
     2461 acctgttgca cataacggct actggctggt tgatggagca gtcgttaacc caattcctat
     2521 ttccctcacg cgtgcattgg gggctgatat tgtgatagcg gttgacctgc agcacgatgc
     2581 tcatttgatg caacaagatt tgctctcctt taatgtcagt gaagaaaata gcgagaatgg
     2641 tgattctctg ccgtggcatg cgcgtctgaa agaaaggtta ggcagcataa cgacacgtcg
     2701 ggcggtgaca gcgccaacgg caacagagat tatgaccact tctatccagg tgctggagaa
     2761 ccgccttaaa aggaaccgca tggcaggtga tccgcccgat attctgattc aacctgtttg
     2821 cccgcaaata tctacgcttg atttccatcg cgcgcacgct gccattgcgg ccggacagct
     2881 ggcagtggaa aggaaaatgg acgaactttt gccgttggta cgcaccaaca tttgaccaga
     2941 atttttatct acacttaagt taattctgac aggcgcaggt ggcaatagca tgccactatt
     3001 gagtaaagcc agtcagggga gagaacatga cgcagccatt ggtcggaaaa cagattctca
     3061 ttgttgaaga tgagcaggta tttcgctcgc ttctggattc atggttttcc tcattgggag
     3121 cgacaacggt actggcggct gatggggtgg atgcccttga gttgctggga ggtttcactc
     3181 cagacctgat gatatgtgat atcgcgatgc cacgaatgaa cgggcttaaa ctgctggagc
     3241 atatacgtaa cagaggcgac cagaccccag ttctggtgat atctgccact gaaaatatgg
     3301 cagatattgc caaagcgtta cgtctgggcg ttgaagatgt tttgctgaaa ccagttaaag
     3361 atctgaatcg cttgcgcgag atggtttttg cctgtctcta tcccagcatg tttaattcgc
     3421 gcgttgagga agaggaaagg ctttttcgcg actgggatgc aatggttgat aaccctgccg
     3481 cagcggcgaa attattacag gaactacaac cgccggttca gcaggtgatt tcccattgcc
     3541 gggttaatta tcgtcaattg gttgccgcgg acaaacccgg cctggtgctt gatattgccg
     3601 cactttcgga aaacgatctg gcattttatt gccttgatgt cacccgagct ggacataatg
     3661 gcgtacttgc tgccttgtta ttacgcgcat tgtttaacgg attattacag gaacagcttg
     3721 cacaccaaaa tcaacggttg ccagagttgg gcgcgttatt gaagcaggta aaccatttac
     3781 ttcgtcaggc caatctgccg gggcagtttc cgctattagt tggctattat catcgcgaac
     3841 tgaaaaatct cattctggtt tctgcgggtc tgaatgcgac gttaaatacc ggcgaacacc
     3901 aggtgcaaat cagtaatggt gttccgttag gcactttagg taacgcttat ttgaatcaat
     3961 tgagccagcg atgcgatgcc tggcaatgcc aaatatgggg aactggtggt cgactgcgct
     4021 tgatgttgtc tgcagaatga gcaaacgata acgcgggcta aatttgcatt acctgctaat
     4081 gtcggctggt ggtactatcg tcgccattcg tataagtaat tgtcttaatt atgctaactc
     4141 gcctcctttt cagaacttag ccccttcggg gtgctgatat actgggatgc gatacagaaa
     4201 tatgaacacg ttcaaaacac gaacagtcca ggagaattta aatggctgcc attaatacga
     4261 aagtcaaaaa agccgttatc cccgttgcgg gattaggaac caggatgttg ccggcgacga
     4321 aagccatccc gaaagagatg ctgccacttg tcgataagcc attaattcaa tacgtcgtga
     4381 atgaatgtat tgcggctggc attactgaaa ttgtgctggt tacacactca tctaaaaact
     4441 ctattgaaaa ccactttgat accagttttg aactggaagc aatgctggaa aaacgtgtaa
     4501 aacgtcaact gcttgatgaa gtgcagtcta tttgtccacc gcacgtgact attatgcaag
     4561 ttcgtcaggg tctggcgaaa ggcctgggac acgcggtatt gtgtgctcac ccggtagtgg
     4621 gtgatgaacc ggtagctgtt attttgcctg atgttattct ggatgaatat gaatccgatt
     4681 tgtcacagga taacctggca gagatgatcc gccgctttga tgaaacgggt catagccaga
     4741 tcatggttga accggttgct gatgtgaccg catatggcgt tgtggattgc aaaggcgttg
     4801 aattagcgcc gggtgaaagc gtaccgatgg ttggtgtggt agaaaaaccg aaagcggatg
     4861 ttgcgccgtc taatctcgct attgtgggtc gttacgtact tagcgcggat atttggccgt
     4921 tgctggcaaa aacccctccg ggagctggtg atgaaattca gctcaccgac gcaattgata
     4981 tgctgatcga aaaagaaacg gtggaagcct atcatatgaa agggaagagc catgactgcg
     5041 gtaataaatt aggttacatg caggccttcg ttgaatacgg tattcgtcat aacacccttg
     5101 gcacggaatt taaagcctgg cttgaagaag agatgggcat taagaagtaa catccgtatc
     5161 ggtgttatcc acgaaacggc gttgagcaat cgacgccgtt tttttatagc ttattcttat
     5221 taaattgtct taaaccggac aataaaaaat cccgccgctg gcgggatttt aagcaagtgc
     5281 aatctacaaa agattattgc ttgatcagga aatcgtcgag ggatttacct tgctcatcca
     5341 ttgctttttt gattacagct ggagtacggc cttggccagt ccaggtttta gtttcgccgt
     5401 tttcgtcaac gtagctatat tttgccggac gctgagcacg tttagctttg gtgccagatt
     5461 taacggcagc aaggctattc agcagttcgt tcgggtcaat accgtcagcg atcagcattt
     5521 cgcgatattg ctgcagttta cgagtgcgct cttcaacttc agcagcagcc gcgctttctt
     5581 cttcgcgacg ttcgttaaca acaacttcta atttttccag catttcttcc agcgtttcaa
     5641 gtgtacattc tcttgcctgc gcacgaagag tacggatgtt gttcagaatt ttaagtgctt
     5701 cgctcattgt agtaatctca aacttatatt ggggtggttt gttgaggtaa taatagagcc
     5761 ttaaattcag ttgtgcaata gccaggaatg taaggaattc aaaattgttc tttattttgt
     5821 gccgccaata aatatctttt cataaaatta gccagaaaag acgcggcata tagccctatt
     5881 tacaccgatg atttcgcagc acgtgaggtt aaaacttcct gattcatgtc acattttatg
     5941 gggagattat cgtaggctga cgacctttca gtcttctgta ttagttgtgt ttacgagaat
     6001 tccctattaa gcgaatgatg aaaagtagaa cagtcgcaat aagagcatgg acttagtatt
     6061 gcactatctc ctggaggtca acagagggct attacttgcg caacaggtta aagattgtga
     6121 atagttacca gcagtcattt acccgcttat aacaagcgag gcagttgtaa tgatagctca
     6181 gaaggattat gcaaggcttc gtaagggaga acgcatatac ccacttctgt gcatactgtt
     6241 gagctgaaaa actgacgaat tatgataaac tccagccaac tttatttcat atcattgagg
     6301 gcctgtggct gatggcacag ctatatttct actattccgc aatgaatgcg ggtaagtcta
     6361 cagcattgtt gcaatcttca tacaattacc aggaacgcgg catgcgcact gtcgtatata
     6421 cggcagaaat tgatgatcgc tttggtgccg ggaaagtcag ttcgcgtata ggtttgtcat
     6481 cgcctgcaaa attatttaac caaaattcat cattatttga tgagattcgt gcggaacatg
     6541 aacagcaggc aattcattgc gtactggttg atgaatgcca gtttttaacc agacaacaag
     6601 tatatgaatt atcggaggtt gtcgatcaac tcgatatacc cgtactttgt tatggtttac
     6661 gtaccgattt tcgaggtgaa ttatttattg gcagccaata cttactggca tggtccgaca
     6721 aactggttga attaaaaacc atctgttttt gtggccgtaa agcaagcatg gtgctgcgtc
     6781 ttgatcaagc aggcagacct tataacgaag gtgagcaggt ggtaattggt ggtaatgaac
     6841 gatacgtttc tgtatgccgt aaacactata aagaggcgtt acaagtcgac tcattaacgg
     6901 ctattcagga aaggcatcgc cacgattaat aagaatttct ttactgacag ggtgagcagg
     6961 gcacttttat cctgtcagtt cgttttacgc acttcttccg ggctatatac ccttctcggc
     7021 agttttttaa cgccgctata cgcctcacag ggctcttaag caccgacgtt gacttgtgac
     7081 ctgtaaagta caatatccct gtgtttaggc gttatacatc gtcgcaaata tgatgaaggc
     7141 taatgctgtc ggtttatgga aaagttgctt tgggtaaaca aaaaatacgg ccccagaagg
     7201 gcaatgccgt tcacttaaga ggagcggcac tatgtttcac aggataacgg gtttttgata
     7261 tcttaaccga cctcggcctt gatggtcggg ggcgttttgt tatgaacacc acttctagag
     7321 caccccgaag atgctccagt cgtttcggga tggtccctgg tgacgccgtg tttcccagct
     7381 ctatcatttc tgatgcgata ttcttccacg caggcagtag ccagtggcgg ttacaaccct
     7441 tctggttcag cgtcagcagc aggtcttcgc tgtaaaaaag tttatcaaac aacgtaatag
     7501 agttatccgg gatggtggcg agcatggagt gggccagcac agtttcgctc tgccggtaag
     7561 gtgcggtcac ggcattcagc agaatgtgac ttcccaggtt cattaaggcc accagacgca
     7621 taaccgggta ggcgttctgc cgcttagtgg atgtgttggc agacccataa tattcacgca
     7681 gctcgggttt atcaggtgtc ctgaactgtg cgccatcaat ggcaaaaagt tgcaggccgt
     7741 gccagtcatc cttcaggtaa cgttccgcgc ccctgtctgt gcggtctggc ggaagagcca
     7801 ttccactggg gcggccccca cgcgctgacg cgcctgggtg acagcgctgc gggccagcag
     7861 gttcatcccc gcttcgccat ccgcgctcag gttcagacgg cgaacaacat cggtaattgg
     7921 ctcattgcac caccatccag ataaccatgt cccccggtaa acgacggcgg cgaacggtcg
     7981 catgagcaga aagcgtcagg cagtgttgta tccactcggt gggaaggtgt tctgcaaata
     8041 gttgtgcaga gggcggaggc ataagcggat ggtcactgaa atcgagcaga tcattgagaa
     8101 gtggcataag aaaacggctc cctgttgtgg aagccgttat agtgcctcag tttaaggatc
     8161 ggtcaactaa tccttaactg atcggcattg cccagaaggg gccgtttatg ttgccagaca
     8221 gcgctactga ttaagcggat tttttcgctt ttttctcagc tttagccgga gcagcttctt
     8281 tcttcgctgc agtttcacct tctacataat cacgaccgta gtaggtatcc agcagaatct
     8341 gtttcagctc ggagatcagc gggtaacgcg ggttagcgcc ggtgcactgg tcatcgaatg
     8401 catcttcaga cagtttatcc acgttagcca ggaagtctgc ttcctgaacg ccagcttcac
     8461 ggatagattt cggaataccc agttcagctt tcagcgtttc cagccatgcc agcagtttct
     8521 cgatcttagc agcagtacgg tcgcccggtg cgctcagacc caagtggtcg gcaatttcag
     8581 cataacgacg gcgagcctgc ggacggtcat actggctgaa tgcagtctgc ttggtcgggt
     8641 tgtcgttcgc attgtagcga ataacgttac aaatcagcag ggcgtttgcc agaccgtgcg
     8701 gaatatggaa ctgggaaccc agtttgtgcg ccattgagtg acatacaccc aggaaggcgt
     8761 tcgcaaacgc gatacccgcg atagtcgctg cactgtgaac acgttcacgc gctaccggat
     8821 ttttagaccc ttcgtggtag gacgctggca gatattcttt cagcagtttc agtgcctgca
     8881 gagcctgacc atcagagaac tcagatgcca gtacagaaac ataagcttcc atggcgtgag
     8941 ttactgcgtc cagaccaccg aaagcacaca gggacttcgg catgtccata accaggttgg
     9001 cgtcgacaat cgccatatcc ggagtcagcg catagtctgc cagcggatat ttctgaccag
     9061 tagcgtcgtc agttacaacc gcaaacggag tgacttcaga acctgtacca gaagtggtgg
     9121 tgacagcgat cattttcgct ttcacgccca ttttcgggaa cttgtagata cgtttacgga
     9181 tatccataaa gcgcagcgcc agctcttcga agtgagtttc cggatgttcg tacataaccc
     9241 acatgatctt cgcggcgtcc atcggggaac caccacccag cgcgataatc acgtctggtt
     9301 tgaaggagtt tgccagttct gcacctttac gaacgatgct cagggtcggg tccgcttcta
     9361 cttcgaagaa gacttcagtt tcaacgcctg ctgctttcag tacggaagtg atctgatcag
     9421 cataaccatt gttgaacagg aagcggtcag tcacgatgag cgcacgtttg tggccatcag
     9481 taatcacttc atccagcgcg attggcaggg agccacggcg gaagtagata gatttcggaa
     9541 gtttgtgcca caacatgttt tcagctcgct tagcaacggt tttcttgttg atcaggtgtt
     9601 tcggaccaac gttttcagag atggagttac caccccaaga accacaaccc agagtcaggg
     9661 aaggtgcgag tttgaagtta tacaggtcac cgataccacc ctgagacgct ggggtgttaa
     9721 tcaggatacg cgccgttttc attttctgac cgaagtaaga aacgcgagcc ggttggttat
     9781 cctggtcagt gtacaggcaa gaggtatgac cgataccgcc catagcaacc agtttctctg
     9841 ctttttctac cgcgtcttcg aaatctttag cgcggtacat tgccagagtc ggggacagtt
     9901 tttcatgtgc gaacggttcg ctttcatcaa caacggtcac ttcaccgatc agaatcttgg
     9961 tgttttctgg tacagagaag cctgccagtt cagcaatttt ataggctggc tgaccaacga
    10021 tagccgcgtt cagcgcaccg tttttcagga taacatcctg aacagctttc agctctttac
    10081 cctgcaacag atagccgccg tgggttgcaa aacgttcacg tacagcgtca taaacagagt
    10141 caacaacaac aacagactgt tcagaagcac agattacgcc gttgtcgaag gttttggaca
    10201 tcagtacaga tgcaactgca cgtttgatat cagcagtttc atcgataaca actggagtgt
    10261 tgcccgcgcc tacaccgata gctggtttac cggagctgta tgcggcttta accatgcccg
    10321 gaccaccagt cgcgaggatc aggttgatgt ctgggtggtg catcagtgcg ttagacagtt
    10381 caacagaagg ttgatcgatc cagccgatca gatctttcgg agcaccggca gcgatagcag
    10441 cctgcagaac gatatcagcc gctttgttgg tggcatcttt tgcacgcggg tgcggggaga
    10501 agataatggc gttacgggtc ttcagactga tcagcgattt gaagatagca gttgaagtcg
    10561 ggttagtggt cggaacgata ccgcaaataa taccgattgg ttcagcgata gtgatggtac
    10621 caaaagtgtc gtcttcagac agaacaccac aggttttttc atctttatag gcgttgtaga
    10681 tatattcaga agcaaagtgg tttttgatca ctttatcttc gacgataccc atgccggatt
    10741 cggcaacggc cattttcgcg agtgggattc gagcatctgc agcagccaga gcggcggcgc
    10801 ggaagatttt gtctacttgc tcttgagtga aactggcata ttcacgctgg gcttttttta
    10861 cacgctctac gagtgcgtta agttcagcga cattagtaac agccataatg ctctcctgat
    10921 aatgttaaac ttttttagta aatcatctgc tcgaatacga gagtatagtc agtgcggtga
    10981 tgatttgctt aacctatgaa aatcaaaagc ttactcgcgc tcacactcac tgtgatttac
    11041 taaaagagtt taaacattag agttattatc tctaatgcgt cacttccagg tggcgtaagc
    11101 aagattactc acttctgggt actgattacg tgatccaaat caaatttttg caaagctgac
    11161 acctttcagc atcgcttttc gccattatag ataacagtta ataaattgta gtatgatttg
    11221 gtggctacat tagcatgttt tgcacaacta gataacaata acgaatgata gcaattttaa
    11281 gtagttagga ggtgaaaaat gctgtcaaaa ggcgtattgt cagcgcgtct tttcaacctt
    11341 atttatggct aacattatcc ggcttttgct tcggagctaa ccgtgattca gacctttttt
    11401 gattttcccg tttacttcaa atttttcatc gggttatttg cgctggtcaa cccggtaggg
    11461 attattcccg tctttatcag catgaccagt tatcagacag cggcagcgcg aaacaaaact
    11521 aaccttacag ccaacctgtc tgtggccatt atcttgtgga tctcgctttt tctcggcgac
    11581 acgattctac aactttttgg tatatcaatt gattcgttcc gtatcgccgg gggtatcctg
    11641 gtggtgacaa tagcgatgtc gatgatcagc ggcaagcttg gcgaggataa acagaacaag
    11701 caagaaaaat cagaaaccgc ggtacgtgaa agcattggtg tggtgccact ggcgttgccg
    11761 ttgatggcgg ggccaggggc gatcagttct accatcgtct ggggtacgcg ttatcacagc
    11821 attaactatc tgtttggttt ctttgtggct attgcattgt tcgctttatg ttgttgggga
    11881 ttgttccgca tggcaccgtg gctggtacgg gttttacgcc agaccggcat caacgtgatt
    11941 acgcgtatta tggggctatt gctgatggca ttggggattg aatttatcgt tactggtatt
    12001 aaggggattt tccccggcct gcttaattaa ttcctttcaa atgaaacgga gctgccatgc
    12061 tccgtttact tcgtcattat ttttactttg ttcccgcgca gttatcaaaa gcaaaaggaa
    12121 taggtaaaaa tattcttctc aaattacagt tagttataag gatttcctta actgcttctc
    12181 ctcaccatca tgttattttc gccacatcat aatcctgggc ttgctgaaga ataattgaaa
    12241 tgatattagt aattccactg cctttggtag aggaaagtgc taaataataa tcaattgtta
    12301 aattattgtg catttcacta ctggaactgt aatcagaaaa gatagacatg cttagccaat
    12361 ctctatttga ttgaattgaa agatgtttgt taaggcatgg atgcaagcta tagattctga
    12421 tacggtcaat aaaagagaat tgcttaacaa ttttgcaaaa tgtattggcg agtaagaacc
    12481 gcatttggta ctttccgggc aaccgccaga cgattcttta ttggtaatga gaataattaa
    12541 caattaaaga gcgtcgcgaa agaataatgt gtctcgacag gggagacaca gtacgaatcg
    12601 acataaggtg atcgtctgaa tcaccagaat aaataaagtc ggtgatagta atacgtaacg
    12661 ataaagtaac ctgacagcag aaagtctccg agcctgtgca gggtcccaat ccgggattac
    12721 acatgctggt taataccagt aattataatg agggagtcca aaaaacaatg accaacatca
    12781 ccaagagaag tttagtagca gctggcgttc tggctgcgct aatggcaggg aatgtcgcgc
    12841 tggcagctga tgtacccaca ggcgtcacac tggcggaaaa acaaacactg gtacgtaaca
    12901 atggttcaga agttcagtca ttagatccgc acaaaattga aggtgttccg gagtctaata
    12961 tcagccgaga cctgtttgaa ggcttactgg tcagcgatct tgacggtcat ccagcacctg
    13021 gcgtcgctga atcctgggat aataaagacg cgaaagtctg gaccttccat ttgcgtaaag
    13081 atgcgaaatg gtctgatggc acgccagtca cagcacaaga ctttgtgtat agctggcaac
    13141 gttctgttga tccgaacact gcttctccgt atgccagtta tctgcaatat gggcatatcg
    13201 ccggtattga tgaaattctt gaagggaaaa aaccgattac cgatctcggc gtgaaagcta
    13261 ttgatgatca cacattagaa gtcaccttaa gtgaacccgt tccgtacttc tataaattac
    13321 ttgttcaccc atcaacttca ccggtgccaa aagccgctat cgagaaattc ggcgaaaaat
    13381 ggacccagcc tggtaatatc gtcaccaacg gtgcctatac cttaaaagat tgggtcgtaa
    13441 acgaacgaat cgttcttgaa cgcagcccga cctactggaa caacgcgaaa accgttatta
    13501 accaggtaac ctatttgcct attgcttctg aagttaccga tgtcaaccgc taccgtagtg
    13561 gtgaaatcga catgacgtat aacaacatgc cgatcgaatt gttccagaag ctgaaaaaag
    13621 agatcccgga cgaagttcac gttgatccat acctgtgcac ttactattac gaaattaaca
    13681 accagaaacc gccattcaac gatgtgcgtg tgcgtaccgc actgaaacta ggtatggacc
    13741 gcgatatcat tgttaataaa gtgaaagcgc agggcgacat gcccgcctat ggttacactc
    13801 caccgtatac tgatggcgca aaattgactc agccagaatg gtttggctgg agccaggaaa
    13861 aacgtaacga agaagcgaaa aaactgctgg ctgaagcggg ttataccgca gacaaaccgt
    13921 tgaccatcaa cctgctgtat aacacctccg atctgcataa aaagctggcg attgctgcct
    13981 cttcattgtg gaagaaaaac attggtgtaa acgtcaaact ggttaaccag gagtggaaaa
    14041 cgttcctcga cacccgtcac cagggtactt ttgatgtggc ccgtgcaggc tggtgtgctg
    14101 actacaacga accaacttcc ttcctgaaca ccatgctttc gaacagctcg atgaataccg
    14161 cgcattataa gagcccggcc tttgacagca ttatggcgga aacgctgaaa gtgactgacg
    14221 aggcgcagcg cacagctctg tacactaaag cagaacaaca gctggataag gattcggcca
    14281 ttgttcctgt ttattactac gtgaatgcgc gtctggtgaa accgtgggtt ggtggctata
    14341 ccggcaaaga tccgctggat aatacctata cccggaatat gtacattgtg aagcactaat
    14401 ggcaatgcgt ggggcaggag tgtcctgctc cacggtgtct gatttttatc gcattacaga
    14461 aggcacaggc cagaaggtag ggcaatgtta aaatttattc tacgtcgctg tctggaagcg
    14521 attccgacgc tatttattct tattactatt tcgttcttta tgatgcgcct cgcgccggga
    14581 agccctttta ccggcgaacg tactttaccg ccagaagtga tggccaatat cgaagcgaaa
    14641 tatcatctta atgatccgat catgacacag tatttcagct acctgaaaca actggcgcac
    14701 ggtgatttcg gtccatcgtt taaatataaa gattattcgg tcaatgacct ggttgcatcc
    14761 agttttcccg tttctgccaa attgggagcc gcagcatttt tccttgcggt aatactgggt
    14821 gttagtgctg gcgttattgc cgcattaaaa caaaacacca aatgggacta taccgtgatg
    14881 gggctggcaa tgaccggggt tgttatcccc agttttgtgg ttgcaccatt attagtcatg
    14941 atatttgcga tcattttgca ttggctgccg ggcggtggct ggaatggtgg ggcgcttaaa
    15001 ttcatgatat tgccgatggt ggcgttgtca ctcgcttata tcgccagtat tgcgcgtatt
    15061 acccgtggct ctatgattga agtattacac tccaacttta ttcgtactgc ccgggcgaaa
    15121 gggttaccta tgcggcggat cattttacgc cacgcattaa aacctgctct gttacccgtg
    15181 ctctcctata tgggccccgc atttgtcggc attattaccg gttctatggt catcgaaacc
    15241 atttatggtt tgccggggat tgggcaactg ttcgttaatg gcgcattgaa ccgtgactat
    15301 tccttagtgt taagcctgac catcctggtt ggtgcgttaa ccattttgtt taatgccatt
    15361 gtcgatgtgc tatatgcggt tatcgacccg aaaatccgtt actgatactg gagctcgcga
    15421 tgatgttaag taagaaaaac agcgagacgc tggaaaattt cagtgaaaag ctggaggtcg
    15481 aagggcgcag cttgtggcag gatgcacgtc gacgttttat gcataaccgt gcggcggttg
    15541 ccagtctgat agtgctggtg ctgatcgcgt tatttgtaat cctggcaccg atgctttcgc
    15601 agtttgccta tgacgatact gactgggcga tgatgtccag cgccccggat atggagtccg
    15661 gtcactactt tggtactgac tcatccggtc gcgacctgct tgtgcgcgtt gcgattggcg
    15721 ggcgtatctc actcatggtc ggtgttgctg cggcactggt ggcagtggtc gtggggacac
    15781 tttatggttc gctttccggt tatctgggcg gtaaagtgga ttcggtaatg atgcgtctgc
    15841 tggaaatcct caactccttc ccattcatgt tcttcgtcat tttgcttgtg acctttttcg
    15901 gtcaaaacat cctgctgatt ttcgtggcga ttggcatggt ttcctggctg gatatggctc
    15961 gtattgtgcg tggacaaacc ctgagtctga agcgcaaaga gtttattgag gcggcacaag
    16021 ttggcggtgt atcgacatcg ggcattgtta ttcgccacat tgtgccgaat gtactcggtg
    16081 tggtggtggt ctacgcatca cttctggtgc ccagcatgat cctctttgaa tctttcctta
    16141 gcttcctggg gttgggtacg caagagccgt taagcagctg gggtgcattg ctgagtgatg
    16201 gcgcgaactc gatggaagtc tctccatggt tattgttgtt cccagcggga ttcctcgtgg
    16261 tgacgctgtt ttgtttcaac tttatcggcg atggcttgcg tgatgccctc gacccgaaag
    16321 atcgttaagg agtgcagcca tgagcgtaat tgaaactgca actgtgccgc tcgcacaaca
    16381 acaggctgac gcactgctga acgtgaaaga tttgcgtgtc acctttagta ccccggacgg
    16441 cgacgtcacg gcggtcaatg atttgaattt ttccctacgt gccggagaaa cgctgggtat
    16501 tgtaggtgag tctggttcgg gtaaatcgca aactgcattt gcgttgatgg gcctgttggc
    16561 tgccaacgga cgtattggcg gatcggcaac cttcaatggg cgtgaaatcc tcaatttgcc
    16621 agagcatgaa ctcaataaac tgcgcgctga acaaatctca atgatttttc aggacccaat
    16681 gacttcgttg aatccctata tgcgcgtcgg tgagcagttg atggaagtgc tgatgctgca
    16741 taagaacatg agcaaagctg aggcgtttga agagtcggtg cggatgctcg atgcggtaaa
    16801 aatgccggaa gcgcgtaaac gcatgaaaat gtacccgcac gaattttctg gcggcatgcg
    16861 tcagcgagtc atgattgcga tggccttgtt atgtcgacct aagctgctga ttgcggatga
    16921 accaacaact gcgctggacg tcaccgtaca ggcgcagatc atgacgctat taaatgaact
    16981 gaagcgggaa tttaataccg ccatcattat gatcacccac gatcttggcg tggtggcggg
    17041 gatctgcgac aaagtgctgg taatgtacgc cgggcgcacg atggaatatg gcaacgcgcg
    17101 cgatgtcttt tatcaacccg ttcatcctta ttctatcggt ttgctcaacg cggtgccgcg
    17161 tctcgatgcg gaaggtgaaa caatgttgac catcccgggt aatccgccaa acctgctgcg
    17221 attaccgaaa ggttgcccgt tccagccacg ttgtccgcat gcgatggaaa tttgtagtag
    17281 cgcaccgccg ctggaagagt ttacgcctgg ccgtctgcgt gcttgcttta aaccggtgga
    17341 ggaactgtta tgaatgctgt aactgaagga agaaaagtcc tccttgaaat cgccgatctt
    17401 aaagtgcact ttgaaatcaa agatggcaaa cagtggttct ggcaaccgcc gaaaacgctc
    17461 aaagccgtcg atggtgtcac tcttcgcctg tatgaagggg aaacattagg tgtggtaggg
    17521 gaatcgggat gcggtaagtc cacctttgct cgcgccatca tcggtttggt caaggcgacc
    17581 gacggtcatg ttgcctggtt aggtaaagag ttgctgggca tgaaacccga tgaatggcgt
    17641 gccgttcgca gtgatattca gatgattttc caggatccgt tggcatcgct aaacccgcgt
    17701 atgaccatcg gcgagatcat cgctgaacca ctgcgtactt atcatccgaa aatgtcacgc
    17761 caggaagttc gcgagcgcgt gaaggcgatg atgctgaaag tcgggttatt gcctaacctg
    17821 attaaccgct atccgcatga gttctctggt gggcagtgcc agcgtatcgg gattgcgcgt
    17881 gctcttattc ttgaaccgaa gctgattatc tgtgatgagc cggtgtcggc gctggacgtg
    17941 tcaattcagg cacaggtggt caacctgctc cagcaattgc aacgtgagat gggattgtca
    18001 ttaattttta tcgctcatga cctggccgtg gtaaaacaca tttccgatcg tgtgttggtg
    18061 atgtatctcg gccatgcggt agaactgggg acctatgatg aggtctacca caatccacta
    18121 catccttaca ccagggcatt gatgtcggca gtccccatac ctgatccgga tctggagaag
    18181 aacaaaacca tccagttact ggaaggggaa ttaccgtcgc cgatcaaccc gccttccggt
    18241 tgtgttttcc gtacccgttg cccgattgcc ggtccagagt gcgccaaaac acgtcctgtt
    18301 ctggagggga gtttcagaca cgccgtttct tgcctgaaag tcgatccact ttaaaaacaa
    18361 taagggctga cagttgtcag ccctttttca cgctaaaagc gattatttat tcccgccaga
    18421 tgatatggca aagtttgtga tctttttcgc ggcataacag aatgcgggca aaaacatcgt
    18481 tgatttcacc atcttcactg tccgccagac caatcaccac ttcggcaaaa aagtccgggt
    18541 tcaaatcgaa atcaacatgt tcctgccagt cttccgccgg atcgaataac tccgcgccgc
    18601 cgcgctcttc aaactgaaga ttgaacagca gaacatcagc tggatcgaga ttgtcggcag
    18661 ccagttcgag aaaaatatcg taggcctgtt caagcgtttc atcttcagtc aggcgattgt
    18721 ttagatccat atccatagtt actacctgtt taacctctgt tggcgacgtt ttacagcaac
    18781 ggactgaaga agtaaaacag tcgctcggcg acacgttgcc ataatggacg ttttagccat
    18841 aaacgggcat cgagcagacg tgaacgcgaa atataatcgt cctgaacggc ggcgaggtca
    18901 gcaccaaaac ctttatcgtc gattgccagg gtaatctcga aatttagcca cagactacgc
    18961 atatcaaggt taactgtgcc aaccagactt agttcgccat cgaccagcac gctcttggta
    19021 tgcagtaacc cgccttcaaa ctgataaatt ttaaccccag cagccagcag ttccgtaaag
    19081 aatgcgcgac tggcccagcc gaccagcatc gagtcatttt ttcgcggaag gataatactg
    19141 acatccaccc cgcgctgcgc cgccgtgcaa atcgcatgaa gtaaatcatc gcttggcaca
    19201 aagtagggcg tggtcatgat caaatattca cgcgccgaat aagccgcagt caataatgcc
    19261 tggtgaatga gatcttccgg aaagccgggg ccagaagcaa ttgtgtgaat ggtgtgaccg
    19321 ctggcctgtt caaacggcat aatattgaca tctggtggtg gcggcagaat acgttttccg
    19381 gtttcaatct cccagtcgca ggaataaata atccccatcg cggtggcgat agggccttcc
    19441 atacgcgcca tcagatcaat ccattgccct acgcccgcat cttgtttgaa gtagcgagga
    19501 tcgaccatat tcatgctgcc ggtgtacgcg atgtaattat cgatcatgat catcttgcga
    19561 tgttggcgca ggtccatacg gcgtaaaaac acacgcatca gattgacctt taaggcttcg
    19621 accacttcaa taccggcatt acgcattagc tcgggccacg ggctgcggaa aaaagccaca
    19681 ctcccggcgg agtcgagcat caatcggcaa tgaatgccgc gtcgcgcagc cgccattaat
    19741 gattcagcca cctggtccgc catgccgccg ggctgccaga tataaaacac catctcaata
    19801 ttatggcgcg cgagctggat gtcgcggatt aacgcctgca tcacatcatc tgactcggtc
    19861 atcagttgta gctgattccc tttgacccca gcgatcccct gacgacgctc gcaaagcttg
    19921 aataatggcg cagcgacact gctattttct tcggcgaaga tatgcttaca ggctttaagg
    19981 tcgttaagcc attttgcggt ggaaggccac atcgctctgg cgcgctcagc gcggcgtttg
    20041 cctaaatgga gctcgccaac ggcaagatag gcaataattc cgactaacgg cagaatgtaa
    20101 ataatcaaca gccaggccat cgcggaggga actgcgcgtc gtttcattag aatgcgtaaa
    20161 gttacgcctg caatgagcaa ccagtatccc agaatggcca accaactcac caacgtataa
    20221 acggttgtca tagattagaa atccttttga aagcgcattg ttatgagttt acgcatatgg
    20281 attcatctgg caaataaaaa cgcgggaaaa gcactggtca gcgtcggggg tgggggtata
    20341 atgaccattc tgttattgca tagagtagtt aacatgaagc ggagtagaac ggaagtgggg
    20401 cgctggcgca tgcagcgtca ggctagccga cgtaaatcgc gttggcttga ggggcaatcg
    20461 cgccgaaata tgcgtatcca cagcatcagg aagtgcattc taaacaaaca gcgtaactcg
    20521 ttattgtttg cgatctacaa tatctaaatg tactgggcac cattgcggtg cccaataagt
    20581 ttactcttcc gtctttaaac tctatttttg cgccgattct ttactgtcag ctttcgtttc
    20641 tagttcatcg ttatcgctga aaataccatg acctgagttc aacaacattg aaacgagcat
    20701 atcattatta atctcttcac catttaaaac tcgcgccaga atttcgctgc caaacagttg
    20761 cggtgagaga atgatatccg gatgtaccat cttaatctta ttcaggtttt tgctatcact
    20821 gacggcgaga actgttttga catcactgct catatctttt gccgagagta caacaaacgc
    20881 gttatctgca tcgttatcac tcagcgccag aatggcccgg caacgatcga ttcccgcttt
    20941 ctttaataca gaactgtcat tactgtcacc ggggataaca tcagcattat cgcctaagcg
    21001 ttgctcaagt tgcttgatat catcttcagg caagttgctg ataaccgtta cgttttgtcc
    21061 gcgttgattc agttgcagga tcgtattgat ggcgagaatc gaatgtccgc aaacaataaa
    21121 atgatcttta cgatgcattg tatgattgtt tccttttaca agtttgttga atcccccgcg
    21181 gataagcgga ccaaaaattg aggtcataga tgtggcaaaa acggtaatgc cggaaataat
    21241 gaccgaaata gtgaacaatc gtgctgattc agaaacaggg acaatatcgc catagccgac
    21301 ggttgacatg gtttctatcg aaaaatagaa cgcggtcatc aaactttcta ttcgcggatt
    21361 aaaaccttcg cttaaataaa gcgcaccgta ggttgagtaa aacagtaatg tcgtgaaact
    21421 aataaatgca aaaattgtcc cggctgcggc gctactgtgg gagaagtctt tgcgcagtat
    21481 cagcaaaaac accagcgtaa aaatgcaaaa tccaatacta aatttcagcc agggataaaa
    21541 atgcagggtg taaattagcg ctatcaacaa caaaatgata ctgattgccc atgcgagctt
    21601 tgcccggaac agtaagccaa tggagttcag gacaagaaag acgccgagca taaagagtgg
    21661 ggcgtttgcc agcaaggaga gatccaactc tgaaaatgct ttgatatgga aaatatcaag
    21721 caaattgacc gacataccat agattgtttt aaaaataagc aatccattta aaaagacggc
    21781 cagcgcgaga atgtcgtgcc gaagggtcac ccataaattt gttgctgttt gtttgaatgt
    21841 agcccagtga ctcactgata cctctgtaac cacaattttg tttaattatc ttagcgtgca
    21901 cattttctga acatacatgc agcgcgtgaa tgtgttaaac aatagtagat gactttatcc
    21961 gcagtaatca aaggatttga gcgagcaact gtacctcagg aaaggtcatt tttcctgaat
    22021 atgctcacat catataaaga aatacagata aagttattat ctgcttgtgg tggtgaatgc
    22081 actgaccggc tataaggaaa ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa
    22141 tattgaatct ctattcagaa cactttctta aatggtttca ctgaaacgtg ttcatagact
    22201 cctgccgcta cgtacgggtc agcatcggcc caggcctgag ctgcttccag cgattcaaat
    22261 tcagcaataa cggttgagcc agtaaatccc gcagcccctg gatcgttact gtctaccgct
    22321 ggcattggac cagctgtcaa caaacgacct tcatcatgca gtaactgtaa acgtgctaaa
    22381 tgtgccggac gaacggaaag gcgtttttcg aggctatcag ctttgtcttg agcgtaaata
    22441 acatacaaca cgggcacaac tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg
    22501 gcgaagatct gcaacggaaa gatgatgtct ttgttaaggc catgcataaa gtaagggtaa
    22561 ttacgccaaa aatgacattt tcactgatcc tgatcgtctt gccttattga atatgattgc
    22621 tatttgcatt taaaatcgag acctggtttt tctactgaaa tgattatgac ttcaatgacc
    22681 cttgatttac ctcgccgctt cccctggccg acgttacttt cggtctgcat tcatggtgct
    22741 gttgtggcgg gtctgctcta tacctcggta catcaggtta ttgaactacc tgcgcctgcg
    22801 cagccgattt ctgtcacgat ggttacgcct gctgatctcg aaccgccaca agccgttcag
    22861 ccgccaccgg agccggtggt agagccagaa ccggaacctg agccgatccc cgaaccgcca
    22921 aaagaagcac cggtggtcat tgaaaagccg aagccgaaac ctaagccaaa accgaagccg
    22981 gtgaaaaagg tacaggagca gccaaaacgc gatgtcaaac ccgtagagtc gcgtccggca
    23041 tcaccgtttg aaaatacggc accggcacgc ctgacatcaa gtacagcaac ggctgcaacc
    23101 agcaagccgg ttaccagtgt ggcttcagga ccacgcgcat taagccgtaa tcagccgcag
    23161 tatccggcac gagcacaggc attgcgcatt gaagggcagg ttaaagttaa atttgacgtt
    23221 acgccggatg gtcgcgtgga taacgtacaa atcctctcag ccaagcctgc gaacatgttt
    23281 gagcgtgagg tgaaaaatgc gatgcgcaga tggcgttatg agccgggtaa gccaggcagt
    23341 gggattgtgg tgaatatcct gtttaaaatt aacggcacca ccgaaattca gtaagcagaa
    23401 agtcaaaagc ctccgaccgg aggcttttga ctattactca acaggtaagg cgcgaggttt
    23461 tccttcagga tcaaccgcga catacttaaa taatgcttct gtcgctttat agcgttgccc
    23521 aattggttca gacgctactt ttttcaccca cacttcaata ttaatgctga ccgatgtcgt
    23581 ccctttctgg acacagcgtg catagcagca caccacatcg ccgaccgcaa ccggccgtaa
    23641 gaaagtcatt ccttcaaccc gcacagtcac tacgcgaccg tgggcaattt cttttgccag
    23701 aatagcgccg ccaatatcca tttgtgacat taaccaacca ccaaagatgt caccattggc
    23761 attggtatcg gcgggcatgg ctaaagtacg taaaacaaga tcgccctgag ggacgttatg
    23821 tgttgtagac atggtaaaac cgacttaaaa ggaaaatcac aggcgcgatg ctactatgat
    23881 tttcctgagg agaacagagg agatacagcg ccagccccga aggactggca gtctggttag
    23941 gatttatctt cctgcggcat gtggcggtag atatagatac cgcttaacaa tgtgaagatt
    24001 aaggtaaggg cggtcaggcc aaagacttta aagttgaccc aaatattttg cggcagccag
    24061 aatgcgatgt agatgtttgc cagaccgcaa aggataaaga aaacagccca ggccagattc
    24121 agcttcgacc ataccggttg cggcagcgtg agttctttac ccagcatccg ctgaattagc
    24181 ggctttttca tcacccattg gctgactaac agggctcccg caaatagagc ataaatgact
    24241 gtaaccttcc atttaataaa ctcatcattg tggaagaaca gcgtcaagcc accgaagacg
    24301 accaccagaa caaaagtgat cagggccatc ttctcaacct tacgaaagcg aacccagcta
    24361 tagataagca caatcgccgt ggcgacgatc agcgccgcag tagccgcata gatgtcataa
    24421 atcttgtaaa acgcgaaaaa gacaaccagc ggtaaaaaat caagaaactg cttcatttta
    24481 cgattccgta atcaaatgct taaggttatt ggcgaatcaa catatacagg cggaacagat
    24541 agatgagcaa tatggctgaa atcaggttgc tcaaggtgtt cgccagtacg gcaccaattt
    24601 ccggggttaa tgcggcaaaa gaagaggcaa aaagcagcaa cagtgttttt gccagcaacc
    24661 agctcagtac tgcgggtgcc accagacgca tattcgccca agtcagccgc atactgctac
    24721 gcatcgaggc aaaaacgccc attttgtcct gtaccaacat caccggtgcc agggccagta
    24781 aaatggccat gataattccc ggaacgacca ccagcatgat gccaatctgt actaaaaggg
    24841 tagtcagaaa aatcagaata aataactttg gcaatatcgg cgcactggca ccaatagctc
    24901 gcagcgcact gactctctga cccgcagaca ccagctggat aattaatatt acgcctccgg
    24961 cgagaatggc gttaccgatt aatcctgaaa aagtggacgc cgctgaagcc tgcagcaaaa
    25021 tttgttgctg ttcctgtgac atattctgaa ccaggtcgaa caacccacta ctgccgctaa
    25081 cgggcacgcc gtcattgagc tgcgcaagct gtgcatcact gggtgagaaa acatgcccta
    25141 acaccactgt gataaacgca catagcaacg ataccaacag aatggtcata aattgattac
    25201 ggaagaaatt tccggtgtca cggtatacgg actgcgccgt gatagacatg cactctcctt
    25261 gagttttatg caggtgttaa ttagcgggca attgtaccct ggttaagcct cgggtggcag
    25321 catcaggtct tgtatggaaa agcatatctt tgtaaagcgg aggtaattgc tggccttcag
    25381 gcatgggcaa atcgcggcaa gtggcgaccg agttaatatt tgcgtagcga aaatatttaa
    25441 aaattgattt aaatcacatt aaccaggatt ctcaatgcaa cttctaaatt aatccagatc
    25501 aataaagggt gaattatcat atgtaatgtg atctatgtag gatcatttgt tactccaatg
    25561 taggtatatt cgtcacgttt ttataaccat aacgacggag cggatatgaa aaagttaaca
    25621 gtggcggctt tggcagtaac aactcttctc tctggcagtg cctttgcgca tgaagcaggc
    25681 gaatttttta tgcgtgcagg ttctgcaacc gtacgtccaa cagaaggtgc tggtggtacg
    25741 ttaggaagtc tgggtggatt cagcgtgacc aataacacgc aactgggcct tacgtttact
    25801 tatatggcga ccgacaacat tggtgtggaa ttactggcag cgacgccgtt ccgccataaa
    25861 atcggcaccc gggcgaccgg cgatattgca accgttcatc atctgccacc aacactgatg
    25921 gcgcagtggt attttggtga tgccagcagc aaattccgtc cttacgttgg ggcaggtatt
    25981 aactacacca ccttctttga taatggattt aacgatcatg gcaaagaggc agggctttcc
    26041 gatctcagtc tgaaagattc ctggggagct gccgggcagg tgggggttga ttatctgatt
    26101 aaccgtgact ggttggttaa catgtcagtg tggtacatgg atatcgatac caccgccaat
    26161 tataagctgg gcggtgcaca gcaacacgat agcgtacgcc tcgatccgtg ggtgtttatg
    26221 ttctcagcag gatatcgttt ttaattccgc acaaaaacga ccccgtaata tacggggtca
    26281 ataaggacat ggtataaagg gtattatttc ttcgcttcta cgccatcagt ttcagagcga
    26341 attaaaaatt tctcagttgt ttgcggaata ttctgaatca gccagtcggc catttgcttt
    26401 tcctcattta aaatcgcttc gatggttgga attgaagctg tatcaccggc attttttgct
    26461 gctgctaata gtgaggtgta acaggcgatt tcaaattgct cgaagacata tccgctaata
    26521 gagcctttga ctatttcatc agaagggaat ataccaccga ttgactgccc aagcgcagcc
    26581 attttactca tggaatcttt aatgactgaa cgtgaaatgt cattacgatc aagaatagtt
    26641 tccagttgaa caatctggtt tttggtttca ctaagatgtt gttcaatacg agcgcgtagt
    26701 tcaggataat tatctatacg gctggccatg gattcaagca tagattcggc ttgcttttcc
    26761 attgcgtggg cgtcacgtaa ccagtcatga taatgttcaa tacgattcat gaaaatgtcc
    26821 tctttataaa atgactaaaa gttaaaattc atatttcagg ctttattttc ggctttctta
    26881 tttacgttat taatggccag atcagtcagt ttgatgtcgg tggccttttc ttcttccagg
    26941 gtttctttca gaagcttcgc tgctttacgg taacctaatt gttcagccag cgtcgctaat
    27001 gtcccgtaac tggcaatctc ataatgctcg actttctgtg ctgcggcaat cagtgcggca
    27061 tcacgcactt cgtttttctc ggtactttcg atgacctcat tagcttcttc aataagacct
    27121 tccattgcca cacatttcat gcgcttaatt ttcaggttcg attcggactc cacaacttga
    27181 tcaatacgtt caatctgtcc atgagtttcc tcgaggtgcg catgaaaagc ctgacttaat
    27241 ttttcatttg atgttgctct tgcgagtttt gccagtgccc gggttaattg tttttctgcg
    27301 ctgtaggtat ctgaaagcag gtgaataaat acatcttcaa tggtcttcat attcatattt
    27361 ttctccagtg aaatcactgc gagccatacg gcccgcagat gcaattatct ttatcagctg
    27421 atattaataa ttaacggtga atcaggattt gcctgattta ttaccaccgc tttgttgacc
    27481 gcctttttta cccgcttcag atgcgcgttg cggatcattt ttaaaattac cgccgctatg
    27541 ctgaccgcct ttacggcctg cgtcggatgc cttctcacgg tcttcggcga aatttcctga
    27601 accaccacga tgttcggcca tgttatttct cccgttgcgt tgcattgttt cattaatatg
    27661 agtgttgtgt gtcgacactc attaaaatta gtcgccaatg agaattagtt aaattaagcg
    27721 caacgagaag atagagggaa aatatatttt gaggaacatt ctggatatat taacaattac
    27781 ctgaggaata agtgacttag agaaaattat tgattttact ggtgttatgt tgcgggatta
    27841 atttgtttga ttatattact gttgggcgga aaaatgacgt aagttgacgt tcgaccgggg
    27901 taagcgaaac ggtaaaaaga taaatattaa atgaatttag gatttttccg gcttcattaa
    27961 agaaagttaa aatgccgcca gcggaactgg cggctgtggg attaactgcg cgtcgccgct
    28021 ttcatcggtt gtacaaaaac tttcagtgcc gccagcattt tctctggctc attaatatgt
    28081 tgctcgatga ttttaacaat ggccgaacca gaaatcgcgc ccgcagctcc tgcatcaatc
    28141 gctgctttta cctgatccgg ggcggaaata ccaaatccct gcaatggagg tgcagcgttg
    28201 tactctttca gcttcgcaac cagatgattg aggggtaacg cggcgcggtt ttctgcgccg
    28261 gtcacgcctg ctcgtgacag caaataggtg taaccacgac cgtaagaggc tatctggcgc
    28321 agcaggtcgt catcggcatt tggcgggcag atgaagatag gtgcgacatt atgacgcaac
    28381 gcggcctggc ggaagggcgc ggactcttca actggcacat cggcaaccag caccgaatcg
    28441 acgccgactt tttcgcactg ggcataaaac tcatcaatgc ctttgttaaa caccagattg
    28501 gcatacatca acaggccaat gggaatggtc gggtgtttct ggcgaatcag tgccagcatt
    28561 tcaaaacatt gtgccggagt cacacctgcc gcaaaggcgc gcagagtggc gttttgaatc
    28621 gtcgggccat ccgccagtgg gtcggagaag gggataccta actccagcgc gtcagcaccg
    28681 gcttcaatta gcgtatcgat aattttcaat gactgctcaa tgcccggatc accgagcgtg
    28741 acgaaaggaa cgaatgcgcc ttctttgcgc tccttcaact gggcaaacag agattcgtag
    28801 cgttccatca gatttcccct cgtgctttca aaatatcgtg aacggtgaag atgtctttat
    28861 cgccgcgacc ggaaaggtta accaccagta gctgctcttt atccgggttt tcgcgcatca
    28921 ttttcaacgc atgggccagg gcgtgggagg attccagcgc cgggatgatc ccttcgtgca
    28981 ggcacagcgt tttgaaggct tcaagggctt catcatcggt aatagacacg taatcagcgc
    29041 gtccagtgct gttaagatac gcgtgttgtg ggccgacaga cgggaaatcc agtccggcgg
    29101 agatggagta agattcttca atctgcccgt cttcggtttg catcatcggc gctttcatac
    29161 cgaaatagat acccacgcga ccatgtttta gcggtgcgcc gtgctcgcca gtttcgatac
    29221 cgtgaccacc tggctccaca ccaatcaggc cgacgttggt ttcattgatg aaatcagcaa
    29281 acatgccgat ggcattcgaa ccgccgccaa cacaggcgat aacggcatcc ggcaggcgac
    29341 cttctctttc cagaatctgc gctttggttt cttcgccaat catccgctga aactcacgca
    29401 caatggtcgg ataaggatgc gggccagctg cggtgcccag catatagtgc gcggtttcgt
    29461 aactaccgga ccagtcgcgc agcgcctcgt tacaggcatc tttcagcgtc gcggaaccgc
    29521 tatgcaccgg gatcacttcc gcacccatta agcgcatacg aaaaacgtta ggcgactggc
    29581 gttcaacgtc tttggcaccc atataaatac ggcatttcag gccgagcagg gcgctggcaa
    29641 gggccgacgc cacgccatgc tgaccggcac cggtttcggc gatgatttcg gttttaccca
    29701 tccgcttcgc cagcaacgcc tgccccagca cctggttagt tttatgcgcg ccgccgtgca
    29761 gcaaatcttc acgcttgaga tacagcgtgg tgttcgtccc ggctgtaatg ttctggcatt
    29821 tggtcagcgc ggttggacgc ccggcatagt ttttcagcag gtcgttgaac tgagcctgaa
    29881 attcaggatc tttttgcgca ctgacaaaag cttcttccag ctggcgcaga gcaggcatca
    29941 ggatttgtgg cacgtacatg ccgccaaact caccaaaata ggggttaagt aatgttgtca
    30001 ttgttccttt ccttaatatg cgcgcagcgt ctggaaaacc gaggccaaaa gacgtgcgtc
    30061 tttgatgccc ggttgcgact ctacagcaga attaaaatca agtccggcgc agccggtttg
    30121 tgccgcttcc acgcagttat ctgcgcctaa gccccccgcc agcagaacgt tgccaagcga
    30181 ttgaccattt aatagtgacc agtcaaaacg ttgcccgctt ccaccctggc cgttgtctaa
    30241 aacatattta tcaacgtgct gaaactcgcg ggcgggcagg gtttcaccga cgcttaatgc
    30301 tttccagatg gcaacatgtg ctggcagagc ttcacgcagc gtatcgatat acagctgttc
    30361 ttcattacca tgcagttgca ctgccgccag cgataacacc ttagctttgt ccaccacatc
    30421 ggcaatatcg tgattgcgga acacgccaac atactgcaac ggtgccgcag ccatcacttc
    30481 ctgcgcctgt tcaacgttga cgcaacgcgg tgatgtcgca acaaaaatca acccaccgta
    30541 aatcgcgccc gcgtcataag ctgctttagc atcttgccca cgcgtcaggc cacatacttt
    30601 attctcaccc agcaacaccc ggcgcacggc ggcgtgcaaa tcgtcatggg ccatcaacgc
    30661 cgaaccaatc agaaaaccgt tagcgaagtg gcttaactcg cgcacctgag cgtaagtatt
    30721 gatgccggat tcgctgatta ccgtcacgtt gtgccccagt ttcggcgcaa gctcgcgggt
    30781 acggttgaga tcaatcgaca aatcacgcag atcgcggttg ttgatgccaa cgacctttgc
    30841 ccccaatgca atggcacgct ccagttcctc ttcattactg acttcggtca gcacacccat
    30901 ctccagactg tgggcgacgg cggcaagctg gcgatattgt tcgtcatcca gtactgaaag
    30961 cattaataag caggcatcag cctggtaata gcgcgccaga tagatctggt aaggatcgat
    31021 aatgaagtct ttacataaaa tcggctgcgg ggcgatttgg ctgacgatgg ggaggaaatc
    31081 aaagctcccc tgaaaatatt tctcatcagt cagcaccgaa attgccgaag cgtaatgttt
    31141 ataaatggcg gcaatgcgtg ctggatcgaa atcatcacgg atcacgcctt ttgacggcga
    31201 cgctttcttg cactccagaa taaacgccgt gcgcgcaccc tgtagcgcat cataaaaatg
    31261 tcgcgtgctc ggctgaacct cattctgaaa actggccagc ggttgctgct gtttgcgggt
    31321 ttctacccaa atcgccttgt ctgcgacgat tttcgctaaa acggtttgca tcatttaccc
    31381 tcgtgccgcc agtgcggtaa ctctgtcgta agcggaacca ctgcgcagta cctcaagaac
    31441 ggtttgcgca ttggcttgca gatcttcatg gccatgcagg cgcattaaca tggcgacgtt
    31501 cgcagcgacg gctgcttcat gggcggcgtc gcctttacct tgtaacaagc gtgttaaaat
    31561 gtcacggttt tcttccggtg ttccgcctgc cagttgctcc tggtggtagg gagtcaggcc
    31621 aaaatcttca gcggtcaatt gatagctctt aatttcgccg tcatgcagct cggcaacgat
    31681 tgtcggcgcg tgtaatgaaa cttcatccat cccgccgctg tgcaccaccg ccgcgcgttg
    31741 ataccccagc acgcgcaagg tttcggcaat cggcagcacc agttccggac tataaacacc
    31801 aattaacgcc agcggcggat gcgccgggtt aatcaatggc cccagcacat tgaacagggt
    31861 gcgggttttc agttgctggc gaaccggcat cgcgtggcgg aatccggtgt gatacttcgg
    31921 cgcaaagagg aaacatacac ctaactcatc cagcgcctgg cgcgatttat cggcgttcat
    31981 atcaagatta ataccgaacg ccgccagcag atcggacgaa ccagatttac tggagacgct
    32041 acggttgccg tgtttcgcca ctttcagccc acaggccgcg gcgacaaacg cactggcggt
    32101 agaaatattg atactgttgc tgccgtcacc gccagtaccg acgatatcag caaacagata
    32161 atccgggcgc gggaacggcg cggcgttttc cagtagcgcg gttgctgctc cggcgatctc
    32221 gttcgggtgc tcaccgcgaa ttttcatgct caccagcgcc gccgccagtt gttccggctt
    32281 cagctcgcca cgcaccaccg ctgaaaacag ctggtggctt tcttgttggc taagcgtctg
    32341 cgcctgatac agtttttcca gaatcggttg cagcgtgttg gctggctcta gtttctgctg
    32401 cgcccaggcc agcgtttgtt ccagcaggcg agcgccctgg gtggtgagaa tggattccgg
    32461 atggaactgg aatccacaaa cgcgatccgc atcgtgacgt actgccatca ccatgccatt
    32521 aaaatgggcg ttgatggtta aaccggccgg aatgttactg ccaaccagcg agtgataacg
    32581 cgccaccggc agcgggtttg ttaatccggc aaacatcgcc tgaccgtcat gttcaatgct
    32641 cgacgcttta ccgtgaagaa tttcgcccgc ctgaccgaca tagcccccgt aagcttcgac
    32701 aattgcctga tgtccgaggc aaatgccaat aatgggcagc ttgccacgca agcgggtgag
    32761 gagttccggc atacaaccgg cttcgctcgg cacaccgggg ccaggagaaa gcatcagtac
    32821 cggattgctc atcgtcgcca ggcgttcaat taaggtctgc gccggaatat ggttgcggta
    32881 aatcaccacg ttatgaccat tgctgcgcaa ctgatctgcc aggttgtacg taaaagagtc
    32941 gatattatcg agcagcagaa tgtcagccat cagaaagtct cctgtgcatg atgcgcggtg
    33001 gcaatagcgc gcagtacagc gcgggctttg ttacgggttt cgtcggcttc cgactgcgga
    33061 acagaatcaa ggactacacc agcacccgct tgcacggtgg cgataccgtt ttccaccagc
    33121 gccgagcgga tcacaatgca ggtgtcgaga tcgccatgcg cggtgaaata acctaccgcg
    33181 ccgccgtagc tgccgcggcg acgaccttcc gcctcggcaa ttaactgcat agcgcgtact
    33241 ttcggcgcac cgcttaacgt ccccatattc atacaggcgc gataagcgtg cagggcgtca
    33301 agatcgtgac gcagttcgcc gactacgcga gagacgaggt gcatcacata ggaataacgg
    33361 tcaactttgg tgagatcggc gacgtagcgg ctgccggggg tgcaaatgcg tgccagatca
    33421 ttacgggcga gatcaaccag catcagatgt tcagacagct ctttatgatc ggtacgcatt
    33481 tccagttcaa tacggctgtc gagatctctg tccagtgaac catcggcgcg acgaccgcgt
    33541 gggcgtgttc cggcaatcgg gtagatctca atctggcggc tggtggcatc atacttgagc
    33601 gagctttccg gcgacgcgcc aaatagggtg aaatcattat cctgcataaa aaacatgtac
    33661 gggctgggat tactcttttt cagcacgtaa taggccgcca gcggtgacgg gcagggcaga
    33721 gagaaacggc gagatggcac cacctggaaa atttctccag cgcgaatcgc tttttgcaac
    33781 aaacgcacta cgccaccgaa ctcttcatcg ctctgattac attcacaacg catatgcggc
    33841 acggaaacca ctggcagcgg cggcgcggct tcggtcagtt gctgacgtag ttcgttcagg
    33901 cgagcagtga gacgttgttt ttcttcttca ttcggagcaa acaggctggc ctgaatacgg
    33961 gtgctttttt tctgatggtc aatcaccatc agcgtttcag cgagataaaa acagaaatca
    34021 gggcagttat tttccgctga cagttgcggt aaatcttcaa atcccgccac aaggtcataa
    34081 gagaacaggc cgccgaagaa catggcttct cgttcttcct tcggtacatt caacagattc
    34141 tgcaataaac ggaaagcgtc aaaaaccgaa agggagcata agcgggcgtc ttcatccagc
    34201 agtggactga caggggggaa gcgcagcaca cggcagtttg gtgattgttc actttccaca
    34261 cccgcaggca gggcgttatc cagtagtgcc agcagggctt cgccgttgcc ggaaagtgcc
    34321 tggattgtga cagtgtcacc taaagctgta atgcgcagcg cactgtctac cagcagcagg
    34381 ctttttaaat catctttgct gtcgatatct gcggattcca gcagcagcgt tgccggacga
    34441 tccccacaca actggtgaaa aagcgcggtg ggattgtcgc gataagcgcc ttcgcaggtt
    34501 agcagttcga gagtcggttt ttgtgtttgc attgttattc tctaattttg ttcaaaaaaa
    34561 agcccgctca ttaggcgggc tgggtatctg attgctttac gcatggtgaa tacactgccc
    34621 gtttcaggaa gtgcgccacc aacctttcag tacgaaaatt gctttcattg tcgataccct
    34681 ttttacgtga acttgcgtac tagttaacta gttcgatgat taattgtcaa cagctcattt
    34741 cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt
    34801 gcgccttgag cgacacgaat tacgcagtga tttacgacct gcacagccat accacagctt
    34861 ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc agtcgagatg cgcgtcggca
    34921 ccctggcgat caccgaccat gacaccacag ctgcaatcgc accagcaaga gaggaaattt
    34981 cacgttcggg actggcgctg aatcttattc ccggcgtgga aatttccacg gtctgggaaa
    35041 atcatgaaat tcatattgtc gggctgaata ttgatattac tcatccgcta atgtgtgagt
    35101 tcctcgcgca gcagacagaa cggcgcaatc agcgggcgca gctcattgcc gaaagacttg
    35161 aaaaagcgca aatacctggg gcgctggaag gcgcgcaacg actggctcag ggcggcgcag
    35221 tgacgcgcgg tcattttgcg cgtttccttg tggagtgcgg caaagccagt tcaatggcgg
    35281 atgtctttaa aaagtatctg gcgcgcggga aaaccggata cgttccgcca cagtggtgta
    35341 caatagaaca agctattgat gtcattcatc attctggcgg taaggcggtg ctagctcatc
    35401 ctgggcggta caatcttagt gctaaatggc tgaaaagatt ggttgcgcat tttgccgaac
    35461 accacggcga cgcgatggaa gtcgcgcagt gccagcaatc gcccaatgaa cgcacccagt
    35521 tggcgaccct tgcgcgtcag catcatttat gggcatcaca aggatctgat tttcatcagc
    35581 catgcccgtg gatcgaactg ggtcgtaaac tctggctgcc cgcaggcgtt gaaggcgtct
    35641 ggcagctatg ggaacagccg cagaacacca cagagaggga attatgagcc agttttttta
    35701 tattcatcct gataacccac agcaacgtct gatcaaccag gcggtggaga tcgtgcgtaa
    35761 aggcggggtg attgtttatc caactgattc cggctatgcg ctcggctgta aaattgaaga
    35821 taaaaacgcc atggagcgta tttgtcgtat tcgccagctg ccggacggtc acaactttac
    35881 cctgatgtgt cgcgatcttt ctgaactctc gacctattca tttgtcgata acgttgcgtt
    35941 tcgtttaatg aaaaacaaca cgccgggcaa ctataccttc atcctgaagg ggacgaagga
    36001 ggtgccacgc cgtctgttgc aggaaaaacg caaaaccatc ggtatgcgtg tgccttctaa
    36061 cccgattgcg caagcgttgc ttgaagcatt gggcgaaccg atgctttcca cttcgctaat
    36121 gctgccaggc agcgaattta ccgaatccga tccggaagaa attaaagacc gtctggaaaa
    36181 acaggtagat ttgattattc acggcggtta tctcgggcag aaaccaacaa cggttattga
    36241 tcttaccgat gatacgccgg tcgtggtgcg tgaaggcgta ggtgatgtga agcctttctt
    36301 ataacaaatt gcgttccatg gatggaagac tatggcaggg aagtttcgct gcattttgct
    36361 gttgatagca gggctgtttg tatcatctct aagttatgca gaaaacacgg agatcccttc
    36421 ttatgaagaa gggatctcgc tctttgatgt tgaagccact ctgcaaccag atggggtgct
    36481 cgacatcaaa gaaaatattc attttcaggc gcgaaatcag cagattaagc acggctttta
    36541 tcgtgattta ccacgactct ggatgcagcc tgatggggac gctgcactgc tgaactatca
    36601 tattgttggc gtcacccgtg atggtattcc tgaaccctgg catcttgact ggcatatcgg
    36661 gttaatgagt attgtcgtgg gcgataaaca acgtttcttg cctcaaggcg actatcatta
    36721 tcaaattcat tatcaggtta aaaatgcttt cctgcgtgag ggggattctg atctgctaat
    36781 ctggaacgtg accggtaacc actggccgtt tgaaatttat aagacccgtt tttctctcca
    36841 gttctctaat attgcgggta atccatttag cgaaatcgat ctctttaccg gagaagaggg
    36901 cgacacatat cgtaatggcc gcatccttga ggacggaaga attgaatccc gcgatccgtt
    36961 ttatcgtgag gatttcacgg tcctctaccg ctggcctcac gctttactta gcaatgcctc
    37021 ggctccgcaa acgacgaata ttttcagcca tcttctttta ccctccacgt catcgttgtt
    37081 aatttggttt ccgtgtctgt tcctggtttg tggatggtta tatctctgga agcgcaggcc
    37141 gcaatttacg ccggtagatg tgattgaaac cgatgtcatt ccgccagatt acacgcccgg
    37201 catgttacgt ctcgatgcga agctagttta cgacgataaa ggtttttgtg ccgatatcgt
    37261 aaatctgatt gtgaaaggaa aaattcatct ggaagatcag tctgacaaga accagcaaat
    37321 cctgattcgt gttaatgaag gcgcgaccag aaataatgcg gtattactgc ccgcagagca
    37381 gttattactg gaagcgttat ttcgtaaagg cgataaggtc gttcttacgg ggagacgcaa
    37441 cagagtctta cgcagggcat ttttacggat gcagaaattt tatctgccgc gtaaaaagtc
    37501 ttcgttttac cggtctgata cttttttgca atggggtgga ctggcaatat tggcggtcat
    37561 tctttacggt aacttgagtc ctgtaggctg ggcaggaatg agtctggtgg gcgatatgtt
    37621 tattatgatc tgctggatta ttcctttttt attttgttcc cttgagcttt tgtttgcccg
    37681 cgatgatgac aagccttgcg ttaatcgtgt aatcatcact ttgtttttac cactgatttg
    37741 ttcaggcgtg gccttctatt ctctctacat caatgtcgga gatgtattct tttactggta
    37801 tatgccagcg ggttatttta ccgctgtttt cctgaccggt tatctcactg gcatggggta
    37861 tatttttctg ccaaagttta cccaaactgg acagcaacgt tatgcccacg gtgaagctat
    37921 cgttaactat cttgcgcgta aagaggcggc aacacacagt gggcggcgtc ggaaagggga
    37981 aacacggaaa ctggattatg cgttgctagg ttgggcggtc tcggcaaacc ttggcagaga
    38041 atgggcagca cgtatcaccc catcactcac agcggctgtt cgcgccccgg aaattgcccg
    38101 tagcggcgtt ttattctcat tacagacgca cctaagttgc ggggcctata ccagtttgtt
    38161 ggggcgaagt tattccggtg
//
LOCUS       DAZFZC010000041        36357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFZC010000041 DAZFZC010000000
VERSION     DAZFZC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.041"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..1326
                     /locus_tag="ACHKXP_002400"
     CDS             49..1326
                     /locus_tag="ACHKXP_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7647383.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLITWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
     gene            1596..2390
                     /gene="yjfZ"
                     /locus_tag="ACHKXP_002401"
     CDS             1596..2390
                     /gene="yjfZ"
                     /locus_tag="ACHKXP_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO7647384.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKTYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2461..2910)
                     /gene="rplI"
                     /locus_tag="ACHKXP_002402"
     CDS             complement(2461..2910)
                     /gene="rplI"
                     /locus_tag="ACHKXP_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO7647385.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(2952..3179)
                     /gene="rpsR"
                     /locus_tag="ACHKXP_002403"
     CDS             complement(2952..3179)
                     /gene="rpsR"
                     /locus_tag="ACHKXP_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO7647386.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3184..3498)
                     /gene="priB"
                     /locus_tag="ACHKXP_002404"
     CDS             complement(3184..3498)
                     /gene="priB"
                     /locus_tag="ACHKXP_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO7647387.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3505..3900)
                     /gene="rpsF"
                     /locus_tag="ACHKXP_002405"
     CDS             complement(3505..3900)
                     /gene="rpsF"
                     /locus_tag="ACHKXP_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO7647388.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDADAGDSEE"
     gene            4227..4502
                     /gene="yjfY"
                     /locus_tag="ACHKXP_002406"
     CDS             4227..4502
                     /gene="yjfY"
                     /locus_tag="ACHKXP_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO7647389.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4632..5318)
                     /gene="ulaF"
                     /locus_tag="ACHKXP_002407"
     CDS             complement(4632..5318)
                     /gene="ulaF"
                     /locus_tag="ACHKXP_002407"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO7647390.1"
                     /translation="MQKLKQQVFDANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(5318..6172)
                     /gene="ulaE"
                     /locus_tag="ACHKXP_002408"
     CDS             complement(5318..6172)
                     /gene="ulaE"
                     /locus_tag="ACHKXP_002408"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO7647391.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSRDQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(6182..6832)
                     /gene="ulaD"
                     /locus_tag="ACHKXP_002409"
     CDS             complement(6182..6832)
                     /gene="ulaD"
                     /locus_tag="ACHKXP_002409"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7647392.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(6846..7310)
                     /gene="ulaC"
                     /locus_tag="ACHKXP_002410"
     CDS             complement(6846..7310)
                     /gene="ulaC"
                     /locus_tag="ACHKXP_002410"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO7647393.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(7320..7625)
                     /gene="ulaB"
                     /locus_tag="ACHKXP_002411"
     CDS             complement(7320..7625)
                     /gene="ulaB"
                     /locus_tag="ACHKXP_002411"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO7647394.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(7641..9038)
                     /gene="ulaA"
                     /locus_tag="ACHKXP_002412"
     CDS             complement(7641..9038)
                     /gene="ulaA"
                     /locus_tag="ACHKXP_002412"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7647395.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQTMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9393..10457
                     /gene="ulaG"
                     /locus_tag="ACHKXP_002413"
     CDS             9393..10457
                     /gene="ulaG"
                     /locus_tag="ACHKXP_002413"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO7647396.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            10565..11320
                     /gene="ulaR"
                     /locus_tag="ACHKXP_002414"
     CDS             10565..11320
                     /gene="ulaR"
                     /locus_tag="ACHKXP_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO7647397.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(11317..12066)
                     /gene="yjfP"
                     /locus_tag="ACHKXP_002415"
     CDS             complement(11317..12066)
                     /gene="yjfP"
                     /locus_tag="ACHKXP_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7647398.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEK
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            12248..12577
                     /gene="bsmA"
                     /locus_tag="ACHKXP_002416"
     CDS             12248..12577
                     /gene="bsmA"
                     /locus_tag="ACHKXP_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO7647399.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIKAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            12726..13001
                     /gene="yjfN"
                     /locus_tag="ACHKXP_002417"
     CDS             12726..13001
                     /gene="yjfN"
                     /locus_tag="ACHKXP_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO7647400.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13118..14743)
                     /gene="aidB"
                     /locus_tag="ACHKXP_002418"
     CDS             complement(13118..14743)
                     /gene="aidB"
                     /locus_tag="ACHKXP_002418"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO7647401.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLSGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(14827..15990)
                     /gene="yjfC"
                     /locus_tag="ACHKXP_002419"
     CDS             complement(14827..15990)
                     /gene="yjfC"
                     /locus_tag="ACHKXP_002419"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7647402.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPLYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(15993..16631)
                     /gene="yjfM"
                     /locus_tag="ACHKXP_002420"
     CDS             complement(15993..16631)
                     /gene="yjfM"
                     /locus_tag="ACHKXP_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO7647403.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRSTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(16641..17039)
                     /gene="yjfL"
                     /locus_tag="ACHKXP_002421"
     CDS             complement(16641..17039)
                     /gene="yjfL"
                     /locus_tag="ACHKXP_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO7647404.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(17057..17716)
                     /gene="yjfK"
                     /locus_tag="ACHKXP_002422"
     CDS             complement(17057..17716)
                     /gene="yjfK"
                     /locus_tag="ACHKXP_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO7647405.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(17767..18465)
                     /gene="yjfJ"
                     /locus_tag="ACHKXP_002423"
     CDS             complement(17767..18465)
                     /gene="yjfJ"
                     /locus_tag="ACHKXP_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO7647406.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLTRVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(18484..18885)
                     /gene="yjfI"
                     /locus_tag="ACHKXP_002424"
     CDS             complement(18484..18885)
                     /gene="yjfI"
                     /locus_tag="ACHKXP_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO7647407.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(19012..19743)
                     /gene="rlmB"
                     /locus_tag="ACHKXP_002425"
     CDS             complement(19012..19743)
                     /gene="rlmB"
                     /locus_tag="ACHKXP_002425"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO7647408.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(19924..22365)
                     /gene="rnr"
                     /locus_tag="ACHKXP_002426"
     CDS             complement(19924..22365)
                     /gene="rnr"
                     /locus_tag="ACHKXP_002426"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO7647409.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPSTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDVRKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(22404..22829)
                     /gene="nsrR"
                     /locus_tag="ACHKXP_002427"
     CDS             complement(22404..22829)
                     /gene="nsrR"
                     /locus_tag="ACHKXP_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO7647410.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(23034..24332)
                     /gene="purA"
                     /locus_tag="ACHKXP_002428"
     CDS             complement(23034..24332)
                     /gene="purA"
                     /locus_tag="ACHKXP_002428"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO7647411.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(24436..24633)
                     /gene="yjeT"
                     /locus_tag="ACHKXP_002429"
     CDS             complement(24436..24633)
                     /gene="yjeT"
                     /locus_tag="ACHKXP_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO7647412.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(24715..25719)
                     /gene="hflC"
                     /locus_tag="ACHKXP_002430"
     CDS             complement(24715..25719)
                     /gene="hflC"
                     /locus_tag="ACHKXP_002430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO7647413.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(25722..26981)
                     /gene="hflK"
                     /locus_tag="ACHKXP_002431"
     CDS             complement(25722..26981)
                     /gene="hflK"
                     /locus_tag="ACHKXP_002431"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO7647414.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(27067..28347)
                     /gene="hflX"
                     /locus_tag="ACHKXP_002432"
     CDS             complement(27067..28347)
                     /gene="hflX"
                     /locus_tag="ACHKXP_002432"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO7647415.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(28423..28731)
                     /gene="hfq"
                     /locus_tag="ACHKXP_002433"
     CDS             complement(28423..28731)
                     /gene="hfq"
                     /locus_tag="ACHKXP_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO7647416.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(28817..29767)
                     /gene="miaA"
                     /locus_tag="ACHKXP_002434"
     CDS             complement(28817..29767)
                     /gene="miaA"
                     /locus_tag="ACHKXP_002434"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO7647417.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(29760..31607)
                     /gene="mutL"
                     /locus_tag="ACHKXP_002435"
     CDS             complement(29760..31607)
                     /gene="mutL"
                     /locus_tag="ACHKXP_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO7647418.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELVLALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(31617..32954)
                     /gene="amiB"
                     /locus_tag="ACHKXP_002436"
     CDS             complement(31617..32954)
                     /gene="amiB"
                     /locus_tag="ACHKXP_002436"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO7647419.1"
                     /translation="MIYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYNVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(32973..33434)
                     /gene="tsaE"
                     /locus_tag="ACHKXP_002437"
     CDS             complement(32973..33434)
                     /gene="tsaE"
                     /locus_tag="ACHKXP_002437"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO7647420.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(33406..34953)
                     /gene="nnr"
                     /locus_tag="ACHKXP_002438"
     CDS             complement(33406..34953)
                     /gene="nnr"
                     /locus_tag="ACHKXP_002438"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO7647421.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLAESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDVGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAERFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            34952..36091
                     /gene="queG"
                     /locus_tag="ACHKXP_002439"
     CDS             34952..36091
                     /gene="queG"
                     /locus_tag="ACHKXP_002439"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO7647422.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(36074..36127)
                     /locus_tag="ACHKXP_002440"
     CDS             complement(36074..36127)
                     /locus_tag="ACHKXP_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647423.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         8783 a         9939 c         8859 g         8776 t
ORIGIN      
        1 ggaaacacca acaaccaggg tttattaata taattaaaag gtttaaacat gaataaaata
       61 aagaattacc ggtggcatat gattgccctc gtatgcttta tcactgtaat caattatctg
      121 gacagaacgg cattaggtat tgcggctcca acgattatgg agacaaccgg aataactaaa
      181 gagcaatatt catggattgt cagtgcattc cagttggcct atacattagg gcaaccggta
      241 atgggcttct ttattgatac cgtgggtctg aagttaagtt ttgcgatatg tgccgcaatt
      301 tggggcctgg cgacaatggg ccatgcactc accggaacgt ggtctggtct ggcatttatg
      361 cgcgccctga tgggtttcag cgaagcgtcg gcaattccgg cgggtgtaaa aaccgcatca
      421 acatggttcc cggcaaaaga gcgtggcgtg gcgacaggtg ttttcaatat gggcacctca
      481 ctcggcgcga tgcttgctcc accgttgatt acctggtgca ttatgtttca tagctggcaa
      541 tttgcgttta ttgtttcagg tagccttgct ttgctcgcgg ctttattttg gttcttttgt
      601 tataaagatc cgaaagatgc caaacgcctt tctgatgaag agcgccacta tattgaatca
      661 gggcaagaac agcatcttaa aacagataag aaagaaaaaa cgtcaatcaa gcatatcctc
      721 agccaacgta atttctgggg gattggcatc gcgcgttttc tcgcagaccc ggcatgggga
      781 accattaact tctgggtgcc gattttcttc gtcgaaacgc tgcattttag cctgaaagaa
      841 attgccatgt tcgtctggct gcctttcctg ctgggcgatc tcggctgttt agccagtggt
      901 tttgtcgcga agttcttcca cgatcgcggc gtgagtttaa ttaactcacg aagaattacc
      961 ttcactattg cagccgtcat tatgatgacg attggcctgg tgagtattgt cgaaaatccc
     1021 tacattgccg tattactgat tagtattggc gcgttctcgc atcaatgtct ttctactgta
     1081 gcagcaactc tgggtggcga tctgttcaaa aaagacgaag tcgctaccgc agtgggtatg
     1141 gcaggagcct gtgcgtggag cggtcagttg attttcaacc tgttcatcgg ggcattcgtt
     1201 cacattatcg gcttcgcgcc gttctttatt gccctggctt tctttgacat tattggtgct
     1261 attgcgttgt ggacgcttat caaagttaaa gatgaagaac cacaagtaca gttagctacc
     1321 cactaattat tttaaataaa aacgctataa ttgaggttat ggcgttttta aagaagcttg
     1381 agttatcgaa cctcgcttac caacgcgttg tcagtcatct cccccctccc tggtgcatcg
     1441 ttatatccgt gatgagcgat ttttgatatt aatatctttc accatccgct attttcacca
     1501 cttcagaaaa caaacttatt tttattaata tcaattcttt ttctttgaac attcaactgc
     1561 gatatttgta aaaatattaa acaggcaaca ataaaatgac attaccaaca actatttatt
     1621 cattccccgc atatttacct cgtttttcgt ccaccgacaa accagcaaaa ctaaaatttc
     1681 atcaatacgc acgcgctaca ttactgtcaa atcgtggaag agatcataat tgcgatggca
     1741 gacgtactgt tgagatccac aaactagatc tctcagactg gcaagcattc aataagttag
     1801 caacgaagtg taatgcttac gatggagtaa caatgaatgg tgataattct tttggatgga
     1861 accatgaagc tacattagat aatattcacg cccagaaata caataaaact tacgcgggtg
     1921 cgaggttaac cgcagaactg aaatatttat tgcaagatgt cgaatccttc gaacccaaca
     1981 gtaaatacac aatacacgag gttgttttgg gacctggtta tagtactcca gactataccg
     2041 gccaaacgat aggatacgtt gtaacccttc ctgcccaaat gcctaattgc tggtcatctg
     2101 aactaccaac aatcgatctt tatatcgacc aacttcgcac tgttacaggc gtttctaatg
     2161 ccctcggatt catcattgct gcattgctaa acgcgtattc tgatttaccc catgatttga
     2221 aaattggtct acgttcctta tcaaattcag cagcaatcta ctcgggttta ggtttcgagc
     2281 gagtgccgca ggaccgagac ataaggagtg atcgtatgca tctaacgcca gcaaaccatc
     2341 ctgacttatg gacacaagag aatggtgaat ggatctactt gcgaaactaa tacctgacga
     2401 aaaatagaaa gcaaaacgcc gaccaatggt cggcgttttt acgtctcgtt gaataacgaa
     2461 ttattcagct actacgttta cgatcacttt cgcgaatact tcgctgtgaa cctggaagct
     2521 cacttcgtgt tcgccagtgg tacgcagaac gccgttcggc agacgaactt cgctcttagc
     2581 cacttcaacg ccagctgcag ttacagcgtc agcgatgtcg cgagtaccga tggaaccgaa
     2641 cagtttacct tcgtcgccag ctttagacgc gatggtaaca gtttccagtg cattgatttt
     2701 ctcagcgcga gcattagcag ctgccagaac ttcagccagt ttagcttcca gttcagcgcg
     2761 acgtgcttcg aagaattcaa tgtttttctt ggtagctgga acagctttac cctgcggtac
     2821 caggaagtta cgagcatagc ccgctttaac gtttacctga tcacccaggc tacccaggtt
     2881 tgctacttta tcaagcagaa taacttgcat taccttatcc tctcaaagtc gtattaatgg
     2941 accgtgaccg attactgatg gcgatcagtg tacggcagca gggacaggta gcgagcgcgt
     3001 ttgatagcgc gagccagctg acgctggtat tttgcacggg taccggtgat acggcttggg
     3061 acaatcttac cgctttcggt gatgtagttt ttcagcgtag cgatatcttt atagtcgatc
     3121 tcttgaacgc cttccgcggt gaaacggcag aacttgcgac gacggaaata acgtgccata
     3181 tggctagtct ccagaatcta tcaattcaat ctgctcggca tgcaaaacca ttttgctcag
     3241 tccgttcttt gccttgtggc atgaaatgaa cccctgaacg gttatgcgac tgccgaccgt
     3301 tatactgtga gtaatggcct ggttttcgtg tccgctaaca ataacgggca tttggcacca
     3361 cgcctgccgg tgaaaaccgg cttcctcctg cacagaacga tgctcaagca cgaactggca
     3421 gtgaggaatt cctgatggac tgacctttcg aaggggagtc ctgcacacgg tgccggacaa
     3481 caccagacgg ttggtcatca gaaattactc ttcagaatcc ccagcatctg catcatctgc
     3541 ggtttcgttt gcgaaatcat cgcgacgctc gcggcgctcg tctttcgctt taaccatcgg
     3601 agatgcttcg gtaacagcgt gcttggtacg cataaccatg ctgcggataa cggcatcgtt
     3661 gaagcggaag gtagtttcca gctcatcgat cacttcctgc ggagcttcaa cgttcatcag
     3721 aacgtagtgt gctttgtgca gtttgttgat cgggtaagcc agctgacggc ggccccagtc
     3781 ttccagacgg tggatcttgc cttctgcacc agtgatggca gcagtgtagc gctcgatcat
     3841 gcccggaacc tgttcgctct ggtcaggatg gaccataaaa acgatttcgt aatgacgcat
     3901 cgaattgctc cttacggatt attcagcctc ctgtctgggt cagccgaatc ccggggaggc
     3961 aaggaacgtg ttaaaggtcg gctgaaaaat gacgcgttat agtacttgta tgccccgcta
     4021 aactcaaggg cggagcagaa ataaatcgcc cctttcgcaa ataacctcta aaccgctgat
     4081 ttggagttca tcacacttca tcatggtgtt tttttgaaca actgcatcag aaatggtcac
     4141 catcaacttc tttatcattg attaaaatta aatcagaaga tcatcacaga ggagagcaca
     4201 caaagtcagc cgtcgaggag gtatctatgt tcagtcgtgt tttagccctt ctggctgtgc
     4261 ttttgctaag tgcaaataca tgggcagcca ttgaaattaa taaccaccag gccagaaata
     4321 tggacgatgt gcaaagctta ggcgtgattt atatcaacca taacttcgcc actgaaagtg
     4381 aagcacgtca ggcattaaat gaagagacag atgcgcaggg cgcaacgtac taccacgtaa
     4441 ttctgatgcg ggaaccgggg agtaacggca atatgcacgc cagcgcggat atttatcgct
     4501 agcaccaggt ataaccaacg aaacattgcc atagtttgct ttgccccctt cgcagggggc
     4561 atttttttga aaaacggctg cggaattaga ccagttatct cccgaagaag gaaatttccg
     4621 cagcgcgtgt tttacttctg cccgtaataa gcgttaggac cgtgcttacg catgaagtgt
     4681 ttattcatca ggaagctgtc gatatgattc agttgtgggt taatgccacg cgcaatccac
     4741 gccattttcg ccacttcttc catcaccacc gcgttatgca ccgcatcgtg ggcatctttc
     4801 ccccaggcaa acggcccgtg ctgatacacc acaattcccg gcgtatgcag cggctcagca
     4861 ttgcccagcg tttcgataat cactttgcca gtgttcagtt cgtactcgcc ctgcacctct
     4921 tcttcgctta atccgcgagt gcacgggatg tcgccaaaga agtagtcggc atgcgtagtg
     4981 cctaacgccg ggatcgccag ccccgcctgc gcccatgcgg tggcatgagt ggagtgggta
     5041 tggacaatgc cgccaagcga cgggtaacga cggtagagtt cgagatgcgt cgcggtgtcg
     5101 gaagacggac gatactcccc ttccaccacc ttgccgctca tatcaaccac caccatatcg
     5161 tccgctttca tggtttcgta ggcaacgccg ctgggcttga tcaccaccag cccgcgttcg
     5221 cggtcgatag cgctgacgtt gccccaggta aaggtcacca gcccgtagcg cggcagatcc
     5281 atgttggcgt caaacacctg ctgttttagc ttttgcatta tgccgcctcc accatgcccg
     5341 ctttcgccat gcgcgctttc acccaatcac gcgctttcgc cacttctgcc gccgggtctt
     5401 ccgccgtttc gctccacatc tcaatcaggt acggcccgca atagccactc tgtttgagcg
     5461 tttcgaaaca acgttcgaaa tccaccacgc cttcgccaaa cggcacgttt ttgaagacgc
     5521 ccggtttggt gtctttcaca tgtaccgcga cgatatgccc gattccggcc tgcaactcca
     5581 tctgcacgtc gttgtcccac gccgagaggt tgccgatatc cgggtagagc tggaaccacg
     5641 ggttgttgag atagtgcgcg tagcccagcg ccttgctgat ggagttcatc aatgggtaat
     5701 ccatgatctc catcgccagc gtcacctgcg cgcggcttgc catctcaacg ctctctttca
     5761 ggccgtcacg aaaacgacga cgcgtttcgt tattggcttc ctgatagtaa acgtcatagc
     5821 ccgccagctg gatcacgcga atgccgacgt cctgagcgaa ctggatagct ttacgcataa
     5881 tctccagccc ctgcgcccgc accgcgtcat cttcgctacc cagcgggaaa cgacgatgag
     5941 cagaaaggca cattgacggt acgcgtacgc cggtttcaac aatcgcgttc accagcgcca
     6001 gacgctgatc gcggctccag tcgaggcgcg acaggcgctc gtccgtttca tctaccgaca
     6061 tctcgacaaa atcgaagccc aacgtttttg ccagttgcag gcgttccagc cagcactccc
     6121 cggcggggag cgctttttca tagatgccaa gcgggatttg tttggacaac atatccgctc
     6181 cttagcccca cagttcggcg atggaacgtt tgaactgacg tgcggcttcc accggagaag
     6241 cggcatcacg gatactacgg cccgcgataa agacgtgaat cggaatgcct ttgaacagtg
     6301 gcagatcttc cagcgccagg ccaccagtga cggtcacttt gaagcccata tcggaaagac
     6361 gtttgatcgc ggtaatgtcc gcttcgcccc acgccacgcc tgcggcctgc gcgtcacggc
     6421 tgcggtgata aaccacctgc tgaatgcctg cgtcgcgcca ctgctgcgcc tgttcccagg
     6481 tccagtagcc ggtcagttca atctgcacgt cgccgttaaa ctcttttgct acgtccagcg
     6541 cgcctttggc ggtgttgata tccgcacagc aaattaccgt cacccagtca gcattggctt
     6601 cgaagcacat acgagaaaga attttgcccg catcggcaat tttggcgtct gcgagtacga
     6661 ttttgtgcgg gtaaagcgct ttcagatcgc gaaccgcacg cacgccttcg cccacgcaca
     6721 gaatggtgcc cacttcgata atgtcgactt cttcggcaat caggcgagtg gtttcgtagg
     6781 cgctatccat agtctggttg tccagcgcga cttgcaacat cggtaatgac atcttcaatt
     6841 ccttcttaag ctgccgcgtt ggtgcggtca atgagatcca gtacttcctg ctcggtacgg
     6901 caggcacgta aacggtcgaa attttcttca tcttcaaaca ggttgacgat ctgcatgatg
     6961 cctacttcct ggtgcgtgtt ggcatcgacc gccgccatgg tgatgaggat atccacaggg
     7021 tcattatctt cgtggttgaa ctccagcggc tttttcagcg tcactagcgc aaaaccggtt
     7081 ttcttaacgc cctcttccgg gcgaccgtgc ggcattgcca ggcctggtgc gagcacaaag
     7141 tacggaccgt gctgttcaac cgcatccaga atcgcctggt agtaacgcgg ctcgacaaca
     7201 tccgccgcca ccagcaggtc aacgccgatt ttcactgcgt cctgccatgt ctctgcttca
     7261 gcctgcaggc ggatggattt attctccgcc agcgaatcac gtaatttcat ggcgcgtcct
     7321 tacttcacgt cctgcgggaa atgctctttg atcacttcca gcagtttcgg gccaaagtcg
     7381 gcaggagaga gcatgttgcg cacgccgacc acgtatttgt tgccggtcac ggtgatttcg
     7441 cccgcaatgt gcgtagaagc gatgatgata tccgcgccgc tcaactcgct tttgtattcg
     7501 ccaaccgcgc agctgtttac cgtgtggtca atgtttgatt gggttaaaaa ctggtccact
     7561 ttcatcttca tgatcatgga actgccttgt ccgttgccac acacagccag aatacgtacg
     7621 gtcataatca aaactcctta ttaagcagac tgttctgcca gttgtttttc tgcatcttct
     7681 tctgcgcgca gtgcgcggcc agcgaagaac atataagcca gtgcaattac aatgatgaca
     7741 gccataaagg cgataccgat ggagaagaag ccctgcatca tcggcggtgc cagaatcgac
     7801 cagtccgcca tacccatcca ggcactcata ccggtaagtt tcaccgccca gacgcaacca
     7861 aagatttcga tcatccccat caccagacaa atcttcagcg ccgcacgcca gccgccgaag
     7921 tggttagcga acacgccgat ggtggcgttg gagaagaaca tcgggataaa gccgggaata
     7981 atcaggatcg aggagccgca ggcgaccagg atgccaaccg caatcagctg accgatggtg
     8041 ccccacataa agccccaaac cacggcgttc ggcgcgaagc tatagatagc cgcacagtca
     8101 atcgccagaa ccgcacctgg gatcaggcgc tgagagatac cgttaaacgc ttcagagagt
     8161 tccgccacaa acatgcgcac gccctgagtg atgatgaaga tcgccaccgc gaaggagaaa
     8221 ccggtttgca ggatgtagac cgtccagttc actttgcctg ccatcgtctg cacggtgtcg
     8281 ataccgaagg agagcagaat ggcgccaaag aagatggtca tgacaatcgc cgtggagacg
     8341 atgttgtcgt ggaaaatgtt cagccagcca ggcagtttga gatcttcaac gctctcctct
     8401 tttttgccca ggaacggcgc gactttataa gcaatccatg atgcaaactg ctgctggtga
     8461 ccgatggaga aaccgcagcc atccgtcact tcctgagtcg gcttgtacat catattggag
     8521 gtaatgcccc agtagagcga aaccagaatc gcggtgcaga taatggtcgt ccacatggaa
     8581 tagccgaaaa taaacaacgt aacggcaatc agccccgcct gctggaacat gatgtgacca
     8641 gtcagcatga tggtacgaat accggtaatg cgacgcagca gcacgtaaca gatgttcagt
     8701 gccagcgcta acaacaccgc gtaacctacc cagctataag catcgcccat gcgttcaatg
     8761 gttgccatca tcgacgcgta agtatctgaa atcgcaccgt tgatgccgta gacttccgac
     8821 attttcgcca ccaccggttt gaaggtactg gtcaggatgc cggaccctgc ctgcaacaac
     8881 atgaaaccaa taatggtttt tatcgtacct ttgataataa cgctgacact tttgcgcagc
     8941 aggatatagc ccagacaggt cacaataccc agcagtaacg gggcattggt catgacctgg
     9001 ttaaaaaaca cggtaaagat gttgtagagg atctccataa cgatctcctg aagaagaggt
     9061 aaccgggtat tccgcacacc cggaatgttg tgcactcact ctaattttca aaagtaatca
     9121 caacaagatt atttatgatt taatgtgacg cgacccgcaa ataattccac acggatagag
     9181 tgggattcac acaacgaaac aattatttat ttaaattcat ttagttaatt taactacaca
     9241 ttccattaaa tctttccaca ctcaaaatca acaacaactt ccctggcaaa aagtcaaaaa
     9301 atggtcattg ccttcctgaa aaaatagtgt caggattaat caaaaccaat cacatattga
     9361 ttcgaattga actatcatga aggtaaatgg cgatgagtaa agtgaaaagt atcacccgtg
     9421 aatcctggat cctgagcact ttcccggagt ggggtagctg gttgaatgaa gaaattgaac
     9481 aagaacaggt cgctcctggc acatttgcga tgtggtggct tggctgcacc gggatctggt
     9541 taaaatcgga aggtggcacc aacgtttgcg ttgatttctg gtgcggcact ggcaaacaaa
     9601 gtcacggtaa cccgttaatg aaacagggtc accaaatgca gcgcatggct ggcgtgaaaa
     9661 aactgcagcc aaacctgcgc accaccccgt ttgttcttga tccgtttgcg attcgccagg
     9721 tcgacgcggt actggcgact cacgatcaca acgatcatat cgacgtcaac gtcgctgctg
     9781 ctgtgatgca gaactgtgct gatgacgtac cgttcattgg accgaaaacc tgtgtggatt
     9841 tgtggattgg ctggggcgta ccgaaagagc gttgcatcgt ggtcaaaccg ggcgatgttg
     9901 taaaagtgaa agacattgaa attcatgcgc ttgacgcttt cgaccgtact gcactgatca
     9961 ccctgcctgc cgatcaaaaa gcggctggcg tactgccgga cggcatggac gatcgcgcgg
    10021 tgaactatct gttcaaaacg cctggcggct ccctgtatca cagcggcgac tcccactact
    10081 ctaactatta tgcgaagcac ggtaacgaac atcagatcga cgtggcttta ggttcatacg
    10141 gcgaaaaccc gcgcggtatc accgacaaaa tgaccagcgc cgatatgctg cgtatgggtg
    10201 aagccctgaa tgcgaaagtg gttatcccgt tccaccacga tatctggtca aacttccagg
    10261 ccgatccgca agagatccgc gtgctgtggg agatgaaaaa agatcgcctg aagtatggct
    10321 tcaagccgtt tatctggcag gttggcggca aatttacctg gccgttggat aaagacaact
    10381 tcgagtacca ctatccgcgt ggtttcgatg attgcttcac tattgaaccg gatctgccgt
    10441 tcaagtcatt cctgtaatct gtcgtttata ccggatagcg tggagtagtt cgcgctatcc
    10501 ggcgtagtat ttcctggcta tttcaaatat catctaaaaa aatcaaattt tatcggaata
    10561 gctcatgact gaagcacaaa gacatcaaat cctcctggaa atgctcgcac aattgggctt
    10621 tgtgaccgtt gagaaagtcg ttgagcgtct gggaatttcg cctgccactg cgcgacgcga
    10681 tatcaataaa cttgacgaaa gcggcaaact gaaaaaagtg cgcaatggcg cagaagctat
    10741 tacccaacag cgcccgcgct ggacgccgat gaatctgcat caggcgcaga atcacgatga
    10801 aaaagtacgt atcgctaaag cggcctcgca gctggttaat ccgggcgaaa gcgtagtcat
    10861 caactgcggc tccaccgcgt ttctgcttgg gcgggaaatg tgtggcaagc cagtgcaaat
    10921 catcactaat tatctaccgc tggcaaatta cctgatcgat caagaacatg acagcgtgat
    10981 cattatgggc ggacagtaca acaaaagtca gtccatcact ttaagcccgc agggcagcga
    11041 aaacagtctc tatgccgggc actggatgtt taccagcggc aaaggactga cagcagaagg
    11101 gttgtataaa accgatatgc tgacggcaat ggcagagcag aagatgctga gcgtggtagg
    11161 gaaactggtg gtactggttg atagcagtaa gattggcgaa cgcgcgggaa tgctttttag
    11221 ccgtgccgat caaattgata tgctgatcac cggcaaaaat gctaacccgg aaatcctgca
    11281 acaactggaa gcgcaagggg tcagcattct gcgtgtttaa agatgctggc ggaaaaatgt
    11341 cactgcagca tctaacgcct caggcgtaat gcggtgacgc acgcctggct gccatgaaca
    11401 ggttagcagt ttatcccgtc ccgtctcgct taaggcctgc tgcaaacgta gtgattcgtc
    11461 ggcaggcaca acgtcatcgt cgaggccatg ccacagcagt agaggtctgt cactaagttg
    11521 ttccaggtgg tttgtcgctt cccactctgc cagcggcgcg acaatgttat tgaattcatt
    11581 ctgctgtgct gtcgtttcag gtatcagcgg tggaaacagt gaacgggcga gtgatgtaaa
    11641 atagcccgat cccatcatgc tggcggtaca tctcaccgtg gggtggcgag cggtaatccc
    11701 cagtgccgtc atcgcgccca tcgacgcacc accgactgcc agacggtcat caagcagcca
    11761 cttttcttcg gctattgccg cacgtaaagt agtgaattcc tgcatacttt gtagcaagat
    11821 ttgccagaat tgatttaacc gccgcgctgc gtcgccacta aaacgactac cgtgatcggg
    11881 cgcatccggc atgatcaccc gcaaaccagc ctgcgccagc gcaacggcaa aatagctata
    11941 caccagactg gacgaagtaa agccgtgata aaaaatcgcg cacggtaacg gggtatcttt
    12001 ttgtccgaca ggataagcat gaagaacggg aatatctgcc agctcgcgtg attctatttc
    12061 aatcatctgt ctctcctttt tgtctggtat gacatgaaga aatccttagc acaagtttct
    12121 gctggcggta ttagttcata atgttgagat gtgggttacg ctttcgtcac gttttcattc
    12181 gaaaatcgcg taccaggtaa caattcggga acattccccc aaagttaaat taataaggca
    12241 ctacactatg gttagcagga aacgtaatag cgttatttac cggtttgcca gtttattatt
    12301 ggtgttgatg ttaagtgcct gtagcgcact gcaaggtacg ccacagccag caccgccagt
    12361 aacggaccat ccgcaagaga ttcgccgcga ccagacgcaa gggttacagc gaataggtag
    12421 cgtaagcacg atggttcggg gttctccgga tgacgcatta gcagaaatca aagcgaaagc
    12481 tgtcgctgca aaagctgatt attacgttgt cgtcatggtg gacgaaacca tcgtgacagg
    12541 acagtggtat tcacaagcca ttttgtaccg taaataaatc agacaactta catagtcagc
    12601 tttacattgc tctgcgtccc tatgcgttta cctgtgcaca ataaattacc ggctggaagc
    12661 ctgggcgttt attaccgcga tggaatgccc tgcaacatgt ggggaaacga aaaatggagc
    12721 tgacgatgaa acaattactt gcctcaccct cgctgcaatt agtgacttat cctgcgagcg
    12781 ccacggcaca gtctgcagaa ttcgccagtg ctgactgcgt gacgggcctg aatgaaatag
    12841 gtcagatctc agttagcaat atttcagggg atccgcagga cgtggaacgt atcgtggcgt
    12901 taaaggccga tgaacaaggt gcgtcatggt accgcattat taccatgtac gaagaccagc
    12961 agcctgataa ctggcgcgta caggcgatac tgtatgcatg agtgtcatct acagcaaatg
    13021 aacaatgccg gatgcggcgc gagcgccttg tccggcctac aattttcaca tatttcattt
    13081 agttaatcga aaccagcgtc gcatcagtcg tatacgctta cacacacact ccccccgtcg
    13141 cccgcagcaa taaatcattc tggatctgct ctgacaaccg tacgccgccg cgcgtatcta
    13201 acatcacctg acaccacgcc tgcgccattg gcggagaagc atatttcaac atttgcgcac
    13261 cgcagcccag caggaatagc tgatgagtaa tctctcgccc cagttcttca gctggcttac
    13321 gcagctgctg ctgtaaacga cgaaccgcgc gatcaaaata gcgatcctgc cctttcactt
    13381 ccacaaatgc ttccgacaat aagtcgtata cgcccgcttg cttattgaga acgcgcaaca
    13441 catccaggca cataatattg ccggaacctt cccaaatact gtttaccggc atctcccggt
    13501 aaagccgcgg cagctcgctc tcctcgcaat aaccaatgcc gcccagcacc tccatcgctt
    13561 cggccacaaa cggcatacct cttttgcaga tcacaaattt tgccgcaggc gtaaataaac
    13621 gcgcccacag ggcttctttg gcatcggcac gccggtccca cgctcgcgca agacgaaaca
    13681 gcaacgccgt ttgcccttca agctgaagtg ccatgcgact taagacatga cgcataaggg
    13741 gctgttggat caatggatta ccaaaaacat ggcgttgatg tgcatgataa atcgccagcg
    13801 aaaatgcacg gcgcatcatg gcatggctac ccagggcgca atcaaaacgc gtcatcccac
    13861 ccattttcag gatcagacga attccttccc cttcctgccc caacaaccaa ccaatggcat
    13921 cctgaaactc cacttcgcaa ctggcgttag agcgattacc cagcttatct ttcagccgct
    13981 cgaggcgaat cgcgttgcgt tgcccgtcag gcaaaaagcg cggcacaaaa aagcaggaca
    14041 gaccacccgc ggtctgcgcc agcaccagat gcgcatcgct ttgcggaacc gagaaaaacc
    14101 atttatgccc caccagccga taagagccat cttccagacg ctctgcacgg gtggtgttgc
    14161 tcataacatc ggaaccgccc tgcttttccg tcattcccat gccaatcaac aaaccgcgtt
    14221 tttgcccacc tgacaataag tgagaatcgt agcgatcgct caatagcggc gtggtccagt
    14281 cctgaaacgg cgcgggtaac atctgtaaca acaatggcgt ggcggcaaag gtcatggtta
    14341 tcggacataa cgaccctgcc tcaacctgcg catgtaacat aaaacgcgcc gcgcgcgcca
    14401 caaatgcgcc ggagcgagcg tcttcttccc aggcaagatt gtgcacccga ttggtacata
    14461 gcgcctgcat cagcaggtgc caggcggggt gaaaacgcac atcgtccaga cggcgtcctt
    14521 gcgcatcgta gcgcaataat tcaggcggat tcacattcgc cagccgcccc agttcaaggg
    14581 attcagccgt tcctaactgc tgaccaatac tggctagaaa atcgctatcc cagccagcac
    14641 cttcacgcgt taccgcttcg cagagcgcgc catcagacag gtataagttg ctgttattta
    14701 atggtatagg ttgattaaaa acggtgtgag tttgccagtg cactgtgtct ccctccatca
    14761 atggcagtca ccattagtat ggtcactgcc atggattcat gacagaaaga gattgctaaa
    14821 acattcttat ccagcaatgt aatgcggaac gaaacgtgag gtgtctttgg tgatcagtgt
    14881 gttatcttca cggatcccca ttccgcacgc ttcatcatcg acaatccagc taccgatgag
    14941 tgtgtagcta tcgccaaacc tcggcagagg ttgaaacgcc tggtagatca tcggttcatc
    15001 ggcgtaatca ccatcagcgt ggtcaacgac gttattctgg ccgtcgaaaa tggtgacatt
    15061 tccgccttcg cgcgagtaga gcggtttacg cacatagctt tcgccagcgg caatctgcga
    15121 tttttcaccc tcaaaccagg atgcaagaag gttaggatga ccagggaaga agcgccaaag
    15181 cagcggcatt agccctttat tactcaagat acttttccat aacggctcca cccattgctc
    15241 gcgacgcttg cgcagcagcg gaccgttatc gtcacgcatc atccactcca gcggatacag
    15301 cttaaatgca cgctggatga cattatcatc aagatcggtc agtacgccgc cgacgcccaa
    15361 accgagatct tcaatgtaga taaaccgcga ctcctgccct gcctgctggg cgcagtcctg
    15421 caagtacagc acggtactcc tgtcttcatc ggtgtcctga cagcagcaaa aataaaacgg
    15481 ttcccgactg taaagctcgc taaagcgtga aatcaggcgt tcctgaatag cattgtactg
    15541 atcggcatca cgcggaataa tgccgctgcg ccgggcatcc tccagccaca gccactggaa
    15601 ataagccgac tcgtacaatg aagttggcgt atcggcgttg tactccagca gcttcaccgg
    15661 cgcattgcca caccaggcaa aatccatccg tccatacagc gaaggatcgc gggcgcgcca
    15721 gctttcagcg atcacatccc agtacaacgg cgggatcgcc agttgcgtca ggatctcttc
    15781 atcttttacc gcgcgatcca ccacctcaag gcacatctga tgcagttccg cagtcggttt
    15841 ttcgatctgc tcttcaatct ggcgcagagt aaaacggtaa gcccgactct catcccaata
    15901 gatttcattg tcgatgatat gaaagtcgaa accgttgtca gcggcgatct ggtccagatc
    15961 ccgtcgcaca ggaacgttgt gtctcagcat gattagcctc cccaatgccc acgggcgctg
    16021 gaagaacgac catagccgcc gcgagaaacg gtagacgctt ttttggtggt aaagccgccc
    16081 gaagagtaag actctttttt gccagaaccg gagcgccaag agtaatcacc agaagtgctg
    16141 cgccagactg ggcgcgaagc aaaagaggaa ccgccgctgt tataaacaaa cggctcatcg
    16201 cgatctttac gaataacccg gcttaacaaa aatccagaaa cgaccgggat ccaactctgt
    16261 tcaacattgt cgtagtagca attttcgtac ttagactgac agttctgctg agtcatattc
    16321 ttcggaacat cggcataaaa tgccgttttg gcgttgttcc agccacgcgc gcaaatgtcg
    16381 gcattattac cgtcatcaat acaatcctgc actgtcgcgt aaaacgttcc gtcgccgtcg
    16441 ttatcgacat cgctactatc gctacaacct ttcaatacga aaaaagcggc accgcccatt
    16501 agcgccaacg ttaagtattt ttgtgcgtag cgattgcgac gcggttcaaa aggattattc
    16561 ggtctaccaa tgcgactaat cgctccgtga ccaattttac tgttatttct tgatttgcgt
    16621 tttctggcca tgatcgtcca ttaccatgtc atacaagctg cgttaaaaat accgccagcc
    16681 agcgcggcgg ttcccataaa cattcctgct gcggtattgt gattaataat tttttcgctt
    16741 aatgcgggca tataaagtct gacaccagca aaaacgagta actgaatcac cagtgcaatc
    16801 ccgccccagg cgaaatagtc tggaatactc accgcattga ttgccgcact ggataagggg
    16861 ataacgtaac ccaacaatgt accgctgaac gccagtgacg ccgcggtatt attgtttttg
    16921 attaactgcc attcgttgtg cggtgtaatt ttagaataga taaacagaaa aataatcacc
    16981 atagccacgc caataaaaaa ataggcgcta aaggcaagaa gtgaatccag tatatgcatt
    17041 gtaaacgtcc ttgtaattaa ccaataatat gaagtgatgt cagcgggata tcgacaccca
    17101 acgcgcgcga aaataaccac tctggctcgc cgagatcgtt aaaagattcc tcaccattta
    17161 gcagcaaata ttcgtaagta tcttcggtta cttgccgttg gtagcccatg gtaaaattat
    17221 ggacctccca tttggcatgg ttttgatttt ctactttttc cagcatgtag acgggttcga
    17281 tatttcccgg ttcttcgctg ttaaaaaatc gctgccagcg tttttcctgc cagtttaagg
    17341 tcattgctcc catcgctttg gcgttgatcg cctcgcgcca gtgactctct ttactgatac
    17401 cgtagctctc ttcatagaca aagagcttga tatcatcaat gtcatcaata tcttcgccgc
    17461 cggtggtatt gatttgcaga aactcatcgc ctgaagtgta gtagcggaaa atctgactac
    17521 cgccgcccag atcgatgtgg ctgacggcgg ctaccgtaaa ttcctcaccc ggcagcgcaa
    17581 tcagcaattc atcttccagc aaacgaaacg ctaacgtgtc gagcgtaaag ccactattga
    17641 gatgaagtcc cagcggacca cgagcgatag ctggcttatt atccttgcca aacagacgct
    17701 ggaaaaaacc agacatattt tatccttaaa atagttgcca gccttttagc gaggctggca
    17761 aaaaaattac tcgccctgtt ggcgttgcag tcttgctaat acatcctggg cgctactttt
    17821 attgctaccg ccaattccgg cttccgccag cttttcgtca aggtcgcgac cgtctgcgac
    17881 tttctccaac tgtgcggcag catccaggcg agcctgacgt tcggcctgac gcgtttgcag
    17941 gcgttttaag gattctgccg ctgtcgaaac gctggaagat gcgccaacgg tagaggttgt
    18001 tacagcctgt tgtgcacgct gcatggcttc agtggcttta acgacttcca tttgctgctc
    18061 aaactgagcg atacgctgcg ctgtcgctgt aactgctttt tccacgccat cacgggagac
    18121 ttccagattc gacaacactt gctcttcagc ggtaatgaga ttctcaaggc gcgcgatttc
    18181 ttcagcaact tcgttaatca acgacggatt aacgttcttg cttaacgctt ccagcgcacg
    18241 ggcttccaga ctggctttgc gctcacgtaa atctttcagc ttatcgtgac ttaatttcac
    18301 ccgcgtcagc agatcaacgc gagattttcc ggctttatcg agttcagctt tagcgtcacg
    18361 aatatgctgt tccagcatgc gcacgccctg ggtttcttca atggattctt ccgcctggga
    18421 gataaacgat ttccccagcg taaataaact ttttaaaatt cccataaata cccgtccttg
    18481 taattagtgt gaatattctt ctgtaatttc agccagatcc aatgcgttat ctaccagcga
    18541 ggttatctca agcaggatat cttcaagaga agatttaagc gataacgccc cgaaaacaat
    18601 gtaatactct tcctgttgta cgctggagat ccctaccgat gacaacggca tcatcttctg
    18661 atttcttaat aagaaggtat taaattcatc tggattgctg atactactca ccggacaaat
    18721 aaaggtttcg ataatcattt ggcgggaagt aaaaagaata ttgatttgca aatcgccata
    18781 atcattcatt ttaataatta atgcgttctc gctatttgtt acatcaatat tttgttcagg
    18841 tacagtttgc agcgccgtcg ctagcgccaa cggattccat gtcatatatt attccatata
    18901 gattaagttt aaatattaat aaaatgaata tttgcaatac gtaattatct taccagctat
    18961 agacaaaaaa aaaccatcca aatctggatg gcttttcata attctgagaa attagctgcg
    19021 ctggcgcacc gcttcaaata agcaaattcc ggtcgcaacc gaaacgttca gggaagaaac
    19081 gcttccagcc atcgggatgc tgatcaactc atcgcaatgt tcacgagtca ggcgacgcat
    19141 accttcacct tccgcaccca tcaccagcgc caggcgtccg gtcattttgc tctgatagag
    19201 tgtatgatcc gcctcgcctg ccgtaccgac gatccagata ttctcttcct gcaacatacg
    19261 catggtgcgc gcaaggttag tcacccgaat cagcggaacg ctttctgccg cgccgcaggc
    19321 tacttttttc gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa tcaccgcatg
    19381 aacgcctgcg gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat cggttacgcc
    19441 gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc agatccggca gatcgttttc
    19501 ctgatactga cgtcctggct tcacgcgggc gataatgccc tgatgcacgg caccgtcgct
    19561 tttctcgtcg agatattggc ggtttgccaa ctggataacc acgccctgag actcaagggc
    19621 gtgaatcagc ggtaacagac gtttatcttc acggcctttt aaaataaaga cttcctgaaa
    19681 acgttcaggg gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct
    19741 cattaatgta ctcgttgttt acgttttgcc tgatgcacta cgtttatccg gtctacatga
    19801 tccctgcaat atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat
    19861 ccggcatgaa caaagcgcat tttgtcagca atctaaccct cttctgtttg aagagggtaa
    19921 tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt
    19981 ctgcgttttc gccgatggct ttttcgcttt tctcacgtct ttcttcgccg ctttcggctt
    20041 cggttttttc tcgccgcgga aggcactgtc tggctcaaag tttacctttt taccgacctg
    20101 acgacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt
    20161 accgacgttg cgcggagcgc gttcgctgga gatcaggcta aagtcgattt tacgctcgtc
    20221 catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct
    20281 ggattcaccc atcagccgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga
    20341 agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc
    20401 agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca
    20461 cttcagccag tcagctacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca
    20521 gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt
    20581 gttgccctga tgcccctgct ctttcgccag cagatactta atggcgcggt gcagcgtcag
    20641 gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccaggccaaa
    20701 gtgaccacgg ttttccggat cgtaaatcgc ctgtttcatc gagcgcagca gcatggtttg
    20761 cagcatttct gcatcaggac gatcggcaac cgactccagc agctccgcgt agtcacgcgg
    20821 ttccggcttg ttaccgcccg gcagttccag ccccagctcc gccagcactg aacggaaaga
    20881 ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt
    20941 ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg
    21001 cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa
    21061 cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac
    21121 tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag
    21181 atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct
    21241 catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc
    21301 gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac
    21361 ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc
    21421 ttctctgtcc agcggcgttg acggacgcac atagtagctg acgtcggcaa tcgcgaccca
    21481 taaacgccag ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc
    21541 gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaga tcaacacggc ccgcttttgc
    21601 ttcttccggc acttcttctt tcagcccggc aacctgttgc tcaacagcct gcggccagat
    21661 gtacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattatc
    21721 gccaaggact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag
    21781 ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat
    21841 atcgaagctc agacggctgt cgtcaggaac cacaaagccg acgcccgctt cggtaaagta
    21901 gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg
    21961 acctttacgg tcagcaccca gcggctgagc cagcacctga tcgccatgaa tgcaggtttt
    22021 catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta
    22081 gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata
    22141 gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg
    22201 caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct
    22261 ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt
    22321 cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt
    22381 gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc
    22441 tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc
    22501 ttagaaagtg cctgtttcaa cctacaggca ggtgtaatgt ggcaaaactc actgctgcaa
    22561 ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc
    22621 ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga
    22681 ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact
    22741 tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat
    22801 ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat
    22861 acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg
    22921 aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttac ccgacacagc
    22981 aatgtatcac actgttcgcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg
    23041 tcgaacgggt cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg
    23101 atcggcacac cagtcagctc ttcaatacgc ttgatatagt tcagcgccgc ctgcggcagg
    23161 ccgctacgat ctttcacgcc gaaggtggat tcagaccagc ccggcatggt ttcgtaaatc
    23221 ggctctacac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc
    23281 ggcatacggt aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta
    23341 gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc
    23401 agccagccgg tacgacgacg acgccccgta gttgcgccga attcgttacc ctgcttgcag
    23461 aggaactcgc cagtttcatc aaacagttcg gtcgggaacg gacctgcacc tacacgagtg
    23521 gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg
    23581 gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg
    23641 atatccagca gcgtaccctg cgcaccttca aacatgacga aatcgccacg ctgacgcgcc
    23701 tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc
    23761 gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg
    23821 aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg
    23881 tcgccaacac gcagaccgcg acgtgctact ttatcttcat aagcaggccc gataccacga
    23941 ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagcgcaacg
    24001 tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg
    24061 ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg
    24121 ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg
    24181 gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca
    24241 acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat
    24301 tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat
    24361 ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat
    24421 cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc
    24481 aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga
    24541 gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac
    24601 cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc
    24661 accgacatcc ctgaggatgc ggtggcttta ttgacctgtg ccgcagtcgt tatattaacg
    24721 cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat
    24781 cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta
    24841 gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc
    24901 accacgcatg atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc
    24961 ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac
    25021 cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg taggcaggtt
    25081 gatctgctta attcgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt
    25141 gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc
    25201 gttatctgcc gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc
    25261 gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag
    25321 gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg
    25381 cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt
    25441 gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt
    25501 gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa
    25561 atgcagaccc ggctcataaa ccagaggttt gttgtcatcg tcacgcagta ccttaccaaa
    25621 acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac
    25681 cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg
    25741 tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac
    25801 gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg
    25861 ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac
    25921 ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac
    25981 actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat
    26041 tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc
    26101 ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact
    26161 tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc
    26221 gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc
    26281 agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg
    26341 ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact
    26401 ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac
    26461 agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc
    26521 tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc
    26581 ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg
    26641 aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg
    26701 gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc
    26761 gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga
    26821 ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg
    26881 cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg
    26941 tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt
    27001 tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct
    27061 acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc
    27121 aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc
    27181 tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggtg gcagacgcaa
    27241 tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat
    27301 ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc
    27361 aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat
    27421 ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg
    27481 cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt
    27541 ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc
    27601 aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc
    27661 aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt
    27721 accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg
    27781 ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg
    27841 attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat
    27901 cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg
    27961 cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat
    28021 atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg
    28081 ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg tcgctttgac
    28141 agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt
    28201 acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa
    28261 ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg
    28321 ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac
    28381 gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
    28441 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
    28501 gccaccggcg ttgttactgt gatgagaaac cgggcgagac gggacaacag tagaaatcgc
    28561 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
    28621 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    28681 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    28741 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    28801 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg
    28861 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    28921 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    28981 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    29041 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    29101 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    29161 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    29221 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    29281 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    29341 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg tacttccggg tctgccgacg
    29401 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    29461 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    29521 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    29581 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    29641 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    29701 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    29761 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    29821 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    29881 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    29941 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    30001 tgtaaatttt gttggcgtaa gggtaaaggc actgccctga tggtcacatg ctgtgcatct
    30061 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    30121 tcttcggcag aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct
    30181 tcacccggcg tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt
    30241 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    30301 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    30361 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    30421 cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    30481 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    30541 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    30601 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    30661 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    30721 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    30781 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    30841 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    30901 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    30961 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    31021 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    31081 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    31141 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    31201 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    31261 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    31321 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    31381 gcatgacgcg ccagcgccag caccagctcg tcttttttga taccgcagcc gttatcacga
    31441 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgtgt cgcacctgca
    31501 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    31561 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    31621 tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgccccctgc
    31681 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    31741 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    31801 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    31861 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    31921 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    31981 aaataggggt cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac
    32041 tgtttctcgt gctgctccag ccagctggcc atttcactgt tggcacgacg gttagagagc
    32101 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    32161 aggaaattgg cgttttgctt acgtgccaca tcactgcgcc ccatcaccga aataaagtaa
    32221 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtgcgcaat
    32281 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    32341 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    32401 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    32461 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    32521 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    32581 atcgtaaaga cgacattgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg
    32641 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    32701 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    32761 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    32821 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    32881 agcgtcgcgg cacccaccgg cgtgcacagc agcagcaacg tcgctaccaa ccaatttctg
    32941 atgcgatata tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    33001 tcacccgcag aggaaactgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    33061 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    33121 cagatggcat cgttggcaaa ataatcgcgg atccccataa actctagctc ctcgggatcg
    33181 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    33241 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    33301 ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    33361 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    33421 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    33481 aaagagatcg gttgccagca tcccgcgcgt cccaaaacgc tccgccagta cgtcagctgc
    33541 cgcaccgtgc gcaacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag
    33601 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    33661 tccgacatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    33721 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    33781 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    33841 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    33901 caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg
    33961 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg caagagagtc
    34021 catcgtcagt tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttttc
    34081 actgcgggtc agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat
    34141 acgaatagcc cccgcagtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc
    34201 tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg
    34261 ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag
    34321 ttgtcctgta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    34381 gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc
    34441 ggaagggata tcaaccgccg caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    34501 gctaatggat tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag
    34561 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    34621 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcactttcct gggccagcag
    34681 cgtgacctca ataccgaccg ctgtggccag ccgcgcgacc acatagccat cgccgccgtt
    34741 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggataagccg aacgacacac
    34801 ctggaatgct gcttcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcgcatc
    34861 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    34921 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    34981 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    35041 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    35101 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattgcc
    35161 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    35221 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    35281 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    35341 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    35401 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    35461 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    35521 ggaaggatgc ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    35581 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    35641 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    35701 gcttatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg
    35761 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt
    35821 aaaagtcacg gaaggatcgg cgattcgtcg tattggtcac ctgcgttggc tgcgtaatat
    35881 cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    35941 taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    36001 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    36061 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    36121 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    36181 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    36241 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    36301 acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat attttattga gagaaga
//
LOCUS       DAZFZC010000042        35707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFZC010000042 DAZFZC010000000
VERSION     DAZFZC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.042"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            195..734
                     /gene="elfA"
                     /locus_tag="ACHKXP_002441"
     CDS             195..734
                     /gene="elfA"
                     /locus_tag="ACHKXP_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7647424.1"
                     /translation="MKKSVLTAFITVVCVTSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADT
                     TQITAFANQASSDAATNVALQMYMNDGTTAITPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            817..1518
                     /gene="elfD"
                     /locus_tag="ACHKXP_002442"
     CDS             817..1518
                     /gene="elfD"
                     /locus_tag="ACHKXP_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7647425.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLAEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNKMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1543..4128
                     /gene="elfC"
                     /locus_tag="ACHKXP_002443"
     CDS             1543..4128
                     /gene="elfC"
                     /locus_tag="ACHKXP_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO7647426.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVELTPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACALLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGPWGTTW
                     YGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLN
                     QLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGK
                     LQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRG
                     QPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETL
                     LDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGS
                     LVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRI
                     ALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIAT
                     LDGVQANSGIIDDDGSLYMAGLPAKGTISVRWGEAPDQICHINYELTEQQINSAITRM
                     DAICR"
     gene            4119..5189
                     /gene="elfG"
                     /locus_tag="ACHKXP_002444"
     CDS             4119..5189
                     /gene="elfG"
                     /locus_tag="ACHKXP_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7647427.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEANTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLYECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5201..5743
                     /gene="ycbU"
                     /locus_tag="ACHKXP_002445"
     CDS             5201..5743
                     /gene="ycbU"
                     /locus_tag="ACHKXP_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7647428.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5751..6266
                     /gene="ycbV"
                     /locus_tag="ACHKXP_002446"
     CDS             5751..6266
                     /gene="ycbV"
                     /locus_tag="ACHKXP_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7647429.1"
                     /translation="MLKRIIWILFLLGLTWGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6232..6969
                     /gene="ycbF"
                     /locus_tag="ACHKXP_002447"
     CDS             6232..6969
                     /gene="ycbF"
                     /locus_tag="ACHKXP_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7647430.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGEALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            7080..8090
                     /gene="pyrD"
                     /locus_tag="ACHKXP_002448"
     CDS             7080..8090
                     /gene="pyrD"
                     /locus_tag="ACHKXP_002448"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO7647431.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8264..8806
                     /gene="zapC"
                     /locus_tag="ACHKXP_002449"
     CDS             8264..8806
                     /gene="zapC"
                     /locus_tag="ACHKXP_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO7647432.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8803..9912)
                     /gene="ycbX"
                     /locus_tag="ACHKXP_002450"
     CDS             complement(8803..9912)
                     /gene="ycbX"
                     /locus_tag="ACHKXP_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO7647433.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10156..12264
                     /gene="rlmKL"
                     /locus_tag="ACHKXP_002451"
     CDS             10156..12264
                     /gene="rlmKL"
                     /locus_tag="ACHKXP_002451"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO7647434.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12275..14182
                     /gene="uup"
                     /locus_tag="ACHKXP_002452"
     CDS             12275..14182
                     /gene="uup"
                     /locus_tag="ACHKXP_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7647435.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14312..15565
                     /gene="pqiA"
                     /locus_tag="ACHKXP_002453"
     CDS             14312..15565
                     /gene="pqiA"
                     /locus_tag="ACHKXP_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO7647436.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15570..17210
                     /gene="pqiB"
                     /locus_tag="ACHKXP_002454"
     CDS             15570..17210
                     /gene="pqiB"
                     /locus_tag="ACHKXP_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO7647437.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17207..17770
                     /gene="pqiC"
                     /locus_tag="ACHKXP_002455"
     CDS             17207..17770
                     /gene="pqiC"
                     /locus_tag="ACHKXP_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO7647438.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGLQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            18026..18193
                     /gene="rmf"
                     /locus_tag="ACHKXP_002456"
     CDS             18026..18193
                     /gene="rmf"
                     /locus_tag="ACHKXP_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO7647439.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18263..18781)
                     /gene="fabA"
                     /locus_tag="ACHKXP_002457"
     CDS             complement(18263..18781)
                     /gene="fabA"
                     /locus_tag="ACHKXP_002457"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO7647440.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18850..20610)
                     /gene="ycbZ"
                     /locus_tag="ACHKXP_002458"
     CDS             complement(18850..20610)
                     /gene="ycbZ"
                     /locus_tag="ACHKXP_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7647441.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20796..21248
                     /gene="matP"
                     /locus_tag="ACHKXP_002459"
     CDS             20796..21248
                     /gene="matP"
                     /locus_tag="ACHKXP_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO7647442.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21324..22376)
                     /gene="ompA"
                     /locus_tag="ACHKXP_002460"
     CDS             complement(21324..22376)
                     /gene="ompA"
                     /locus_tag="ACHKXP_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO7647443.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(22733..23242)
                     /gene="sulA"
                     /locus_tag="ACHKXP_002461"
     CDS             complement(22733..23242)
                     /gene="sulA"
                     /locus_tag="ACHKXP_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO7647444.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23461..24090
                     /gene="sxy"
                     /locus_tag="ACHKXP_002462"
     CDS             23461..24090
                     /gene="sxy"
                     /locus_tag="ACHKXP_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO7647445.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(24053..26215)
                     /gene="yccS"
                     /locus_tag="ACHKXP_002463"
     CDS             complement(24053..26215)
                     /gene="yccS"
                     /locus_tag="ACHKXP_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO7647446.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDQSQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26225..26671)
                     /gene="yccF"
                     /locus_tag="ACHKXP_002464"
     CDS             complement(26225..26671)
                     /gene="yccF"
                     /locus_tag="ACHKXP_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO7647447.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26794..28848
                     /gene="helD"
                     /locus_tag="ACHKXP_002465"
     CDS             26794..28848
                     /gene="helD"
                     /locus_tag="ACHKXP_002465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO7647448.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28880..29338)
                     /gene="mgsA"
                     /locus_tag="ACHKXP_002466"
     CDS             complement(28880..29338)
                     /gene="mgsA"
                     /locus_tag="ACHKXP_002466"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO7647449.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29434..30096)
                     /gene="yccT"
                     /locus_tag="ACHKXP_002467"
     CDS             complement(29434..30096)
                     /gene="yccT"
                     /locus_tag="ACHKXP_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO7647450.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30269..30682
                     /gene="yccU"
                     /locus_tag="ACHKXP_002468"
     CDS             30269..30682
                     /gene="yccU"
                     /locus_tag="ACHKXP_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO7647451.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQKGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30727..31044)
                     /gene="hspQ"
                     /locus_tag="ACHKXP_002469"
     CDS             complement(30727..31044)
                     /gene="hspQ"
                     /locus_tag="ACHKXP_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO7647452.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31102..32292)
                     /gene="rlmI"
                     /locus_tag="ACHKXP_002470"
     CDS             complement(31102..32292)
                     /gene="rlmI"
                     /locus_tag="ACHKXP_002470"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO7647453.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32387..32665
                     /gene="yccX"
                     /locus_tag="ACHKXP_002471"
     CDS             32387..32665
                     /gene="yccX"
                     /locus_tag="ACHKXP_002471"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO7647454.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32662..32991)
                     /gene="tusE"
                     /locus_tag="ACHKXP_002472"
     CDS             complement(32662..32991)
                     /gene="tusE"
                     /locus_tag="ACHKXP_002472"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO7647455.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(33082..33741)
                     /gene="yccA"
                     /locus_tag="ACHKXP_002473"
     CDS             complement(33082..33741)
                     /gene="yccA"
                     /locus_tag="ACHKXP_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO7647456.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34149..35168)
                     /locus_tag="ACHKXP_002474"
     CDS             complement(34149..35168)
                     /locus_tag="ACHKXP_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7647457.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(35146..35388)
                     /locus_tag="ACHKXP_002475"
     CDS             complement(35146..35388)
                     /locus_tag="ACHKXP_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7647458.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELFSAEPDFEALNNGKTKKK"
     gene            complement(35456..>35707)
                     /locus_tag="ACHKXP_002476"
     CDS             complement(35456..>35707)
                     /locus_tag="ACHKXP_002476"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7647459.1"
                     /translation="NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFD
                     ARTTIPFEGERHNALNDARYQAKYVSAIWQKLLPSQADF"
BASE COUNT         9203 a         8458 c         9235 g         8811 t
ORIGIN      
        1 atagtcatta gatgttgaat caatgaatta ctcgttcagt taatatttaa atattcatga
       61 aatctataaa ttaaagattt gtcacttatt ggatttagta tgcttgattt aaatggcgga
      121 caattattgt tattattaag tctaatgtca tttaaaggca ttatcatatt tacgaagaga
      181 tcaggatgat aacgatgaaa aaaagtgtat tgacggcgtt tataactgtg gtatgtgtaa
      241 cgtccagcgt tatggctgct gatgataatg ctatcacgga tggctcagta acatttaatg
      301 gtaaagttat tgctccagct tgtaccctgg tagctgcgac gaaagattcc gtggtgactt
      361 tgccagatgt tagtgccacg aagttgcaaa ccaatggtca ggtttctggc gtgcaaactg
      421 atgtgccaat tgaattaaaa gattgtgata ctaccgtaac aaaaaatgca acgttcacct
      481 ttaatggcac tgcggatact actcagatta cagcgtttgc taaccaggcc tcatctgatg
      541 ctgctacaaa cgtggccctg caaatgtata tgaatgatgg tacaacggcc atcacgccag
      601 acacagaaac cgggaacatt ttgttgcagg atggagatca gacgttgact tttaaagttg
      661 attatatcgc tacggggaaa gcgacttcag gtaatgtgaa tgcggtaaca aatttccata
      721 ttaactatta ttaatagaac tcattaattg ttttattaat tagtacccct ccagtgttct
      781 ggaggggata ttcatatttt ttaagagtga ctatttatga aaacttgcat aacaaaggga
      841 attgtgaccg taagtttaac ggcaatatta ctcagctgct catccgcatg ggcggcaggt
      901 aaaggcggga ttggacttgc agcaacacgt cttgtttatt cagaaggtga agagcaaatt
      961 tcactgggtg tacgtaacac cagtccggat gttccttatc ttattcagtc atgggtgatg
     1021 accccagata ataaaaaatc agcagacttt attattacac caccgctatt tgtgctgaat
     1081 ccggcaaatg agaatctgtt acgcattatg tacattggag cgccgttggc gaaagacaga
     1141 gaaacccttt tcttcactag cgtacgggca gtcccttcaa caacgaagcg ggaagaggga
     1201 aataccctga agattgccac acaaagcgtc atcaaacttt tctggcgacc aaaaggttta
     1261 gcgtatccct tagccgaggc tccggcgaaa ctgcgttgca cttcgtcagc tgacatggtt
     1321 acggtcagta acccaacacc ttatttcatt accctgacag acctgaaaat aggtggaaag
     1381 gtagttaaaa ataaaatgat ttcccccttt gataaatacc aattctctct gccaaagggt
     1441 gccaaaaata gcagcgtaac gtatcgaacc atcaatgact acggggcgga aacgccgcaa
     1501 ctcaactgta aatcgtaatt cgtcttcagt taagagagcg agatgtatag aactcaccga
     1561 caacacagcc tgttaagctc tggtggagtg ccatcgttta ttggtgggct ggtggtgttt
     1621 gtgtcggcag cgttcaatgc acaagctgaa acctggttcg atcctgcctt tttcaaagat
     1681 gatccctcaa tggtggccga tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg
     1741 gtttatcgtg tcgatattgt tctgaatcag acaattgtag atacgcgcaa cgtcaatttt
     1801 gttgagttaa cgccagagaa ggggattgcc gcctgtttga cgactgaaag cctggatgca
     1861 atgggtgtga atactgatgc gtttccggct tttaaacaac tggacaaaca agcgtgtgcg
     1921 ctattggcgg agattattcc ggatgccagc gtaactttta atgtgaataa actccgtctg
     1981 gaaatttcag taccgcaaat tgctataaaa agtaacgctc gtggttatgt cccccctgaa
     2041 cgttgggatg aagggatcaa cgcgctatta ctgggatatt catttagcgg ggctaacagt
     2101 attcatagca gcgcagacag tgattctggc gacagttatt ttctgaattt aaacagtggc
     2161 gttaatttag gcccatggag attgcgcaac aattcaacat ggagtcgcag tagtggccaa
     2221 accgcagaat ggaagaatct cagcagctat ttgcagcggg cggttattcc actaaaaggc
     2281 gaactgaccg taggtgatga ttatactgca ggcgattttt tcgatagtgt cagctttcgt
     2341 ggtgtgcagc tggcgtcaga tgacaacatg ctgccagaca gcctgaaagg gtttgcgcct
     2401 gtggtgcgtg gtatcgccaa aagcaatgcc cagataacga ttaagcaaaa tggttacacc
     2461 atttaccaaa cttatgtatc gcctggtgct tttgaaatta gtgatctcta ttccacgtcg
     2521 tcgagcggtg atttgttagt tgaaatcaaa gaagcggacg gcagcgtcaa tagctacagc
     2581 gtgccattac tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga
     2641 accaatagta atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga
     2701 ccgtggggaa cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg
     2761 tttggtctgg gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg
     2821 aagagtacgc tggcagacca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc
     2881 aaaacgctca accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg
     2941 ggtttctaca ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac
     3001 ggtgatgatg aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt
     3061 ggcaaactgc aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt
     3121 ggtagccagc aaacttactg gcataccgat caacaggatc ggctattaca gtttggctac
     3181 aacacgcaaa ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt
     3241 caacctgatg ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc
     3301 cccagaagta atgatagcta taccaggaaa aaaaattacg cctggatgac ctctaacacc
     3361 agtatcgata acgaagggca cattacacaa aacctgggtt taacggagac actactcgat
     3421 gacggtaacc tgagctacag cgtgcaacag ggatataaca gcgaggggaa aacggctaat
     3481 ggtagcgcca gcatggacta caaaggggcg tttgcagatg cccgagtggg ctacaactac
     3541 agcgataacg gcagtcaaca acaactgaac tacgctcttt caggcagttt agttgcccat
     3601 tcacagggca ttaccctggg gcaatcgctg ggggaaacta acgttctgat tgcagcacca
     3661 ggcgcagaga atactcgtgt ggcgaacagc accgggctga aaactgactg gcgcggatat
     3721 accgttgttc cttatgccac ttcttatcgg gaaaatcgaa tcgcacttga tgcggcgtcg
     3781 ttaaaacgta acgtggatct tgaaaatgca gtagtcaacg tggttcccac caaaggggcg
     3841 ttggttctgg cggagttcaa tgcccatgcg ggtgcaaggg tattaatgaa aacatcaaag
     3901 cagggtatac cgctgcgttt tggcgcgata gcgacgctgg acggcgtaca ggctaatagc
     3961 ggcataattg atgatgatgg ctcgctctat atggcgggtt taccggcgaa gggaacaata
     4021 agcgtgcgct ggggcgaagc tcccgatcaa atttgtcata tcaattacga gcttaccgaa
     4081 caacaaatta actctgcgat tacgcgaatg gatgccatat gcagataatc tttggagaaa
     4141 aatgcgtgtc attactacga ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa
     4201 ccgctgctta tagcgggcag tgtcatacta ctcaggggaa tccgtatatt ggcgtcaatt
     4261 ttggcgttaa aaccctggag gaagaagcaa atacggcagg ggtagttaaa gacaaatttt
     4321 atcagtggaa cgaatcgaat gattattatg tttcctgtga ttgcgataaa gacaatgtca
     4381 gaagtggccg atgggcattc gccgcggatt caccgttagt ctatttaggc gacaactggt
     4441 acaaaattaa tgactatctt gccgccaaag ttttattgca ggttaaaggc agttctccta
     4501 ctgcggttcc tttcgaaaac gtgggcacag ggggggatac ccgatggcat atttgcgacc
     4561 ctggcggtca acgtttaggt gggcaggggg caagcggtaa tagcggtagc ttttccctga
     4621 aaatattgca gccgttcgtt ggctcggtcg tcattcctcc tatggcgctg gcgcgattat
     4681 atgaatgcta caacataccc gcaggtgatt cctgcacgac tacaggtaca ccggttttag
     4741 tgtattacct gtctggtacg atcaattcac ttggctcatg ttccgtcaat gccggagaga
     4801 caattgaagt tgatttaggt gatgtcttcg ctgccaattt ccgtgttgta gggcataaac
     4861 ctcttggggc cagaacagca gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg
     4921 gattagttaa tgtcaacctg agtctgacgg caaccacaga ccccagctat ccccaggcga
     4981 ttaagacgtc acgtcctggc gtgggcgtgg tggtgaccga tagccagaac aacattattt
     5041 cccctgctgg tggaacatta ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa
     5101 tgaatgtcta tccagtcagc acgacaggtg taccaccaga aaccgggcga tttgaagcca
     5161 cggcaacggt gagaataaat tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt
     5221 tcagtgcgtt atcttgttct ttagcattct taacatccat gtcgggatgg ctgggcctga
     5281 acaagttagt atgcatattt atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa
     5341 aagtgcatta caaaatattc atattggtga ttttaatatc agtgattttc aggccgcgaa
     5401 taccgtaagc actgctgctg atttgaatat cgatatcacc ggttgtgccg ctggtattac
     5461 tggcgcggac gtccttttta gcggcgaggc tgacaccctt gcgccgacac tgctcaaact
     5521 aactgacaca ggcggaagcg gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc
     5581 gcaaagtcag caagaaatcc cgctcaatca ggtccagcct cttacgccct taaaagccgg
     5641 ggataacaca ctcaaatatc aacttcgtta taagtccaca aaggcgggag caacgggcgg
     5701 taatgcgacg gcggttctct attttgatct ggtttaccag tgaaggagtg atgttgaaac
     5761 ggataatctg gattctgttc ttattgggat taacgtgggg ctgtgagcta tttgcccatg
     5821 atggcacggt caacattagc ggatcgtttc gccgtaatac atgcgtgctg gcacaggata
     5881 gcaagcaaat caatgtgcag ttaggcgatg tctcgctgac acgtttttct catgggaatt
     5941 atggcccgga gaaatctttc atcattaatt tgcaggattg cggaacagat gtcagcacgg
     6001 ttgacgtcac cttttcagga acccccgacg gagtgcagag cgaaatgttg tccattgaaa
     6061 gtggtacgga tgcagccagc gggctggcaa ttgcgattct ggatgatgcg aagatattga
     6121 ttccgcttaa ccaggcgagt aaagactaca gcctacatag cggtaaggtg ccattaactt
     6181 tttatgcgca attgcgacct gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg
     6241 cgacatttgt acttcattat gactaatacg tggaacagat tggcgctctt gattttcgcc
     6301 gttttatcgc tgctggtggc gggtgaattg caggcaggag tggtggtcgg cggaacgcga
     6361 tttatctttc cggcagacag agaatcgata tctattttac tgactaatac cagtcaggaa
     6421 tcctggctta ttaacagtaa aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg
     6481 acagtgccag caccattact ggccgctccg ccacttattc tcctgaagcc cggtacgaca
     6541 ggcacgttgc gcttgctgag aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta
     6601 tttgagttaa gcattgccag cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg
     6661 gcgatgcgct cggtatttaa actgttctgg cgaccagaag gtttgccggg cgacccgctg
     6721 gaagcttatc aacaattacg ctggacacgg aattcgcagg gtgtacaact cactaaccca
     6781 acgccttact acattaacct gattcagttg agcgtaaatg gtgaagcgtt aagtaatgcg
     6841 ggagttgtgc cgcctaaaag ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt
     6901 catgtcgcct ggcgggcgat aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat
     6961 ctgccctgaa acaggttcgg aaaacgtttg cgtttttttt gccgcaggtc aattcccttt
     7021 tggtccgaac tcgcacataa tacgcccccg gtttgcacac cgggaatcca ggagagttca
     7081 tgtactaccc cttcgttcgt aaagcccttt tccagctcga tccagagcgc gctcatgagt
     7141 ttacttttca gcaattacgc cgtattacag gaacgccgtt tgaagcactg gtgcggcaga
     7201 aagtgcctgc gaaacctgtt aactgcatgg gcctgacgtt taaaaatccg cttggtctgg
     7261 cagccggtct tgataaagac ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat
     7321 cgatcgagat cggtaccgtc acgccacgtc cacagccagg taatgacaag ccgcgtctct
     7381 ttcgtctggt agatgccgaa ggtttgatca accgtatggg ctttaataat cttggcgttg
     7441 ataacctcgt agagaacgta aaaaaggccc attatgacgg cgtcctgggt attaacatcg
     7501 gcaaaaataa agatacgcca gtggagcagg gcaaagatga ctatctgatt tgtatggaaa
     7561 aaatctatgc ctatgcggga tatatcgcca tcaatatttc atcgccgaat accccaggat
     7621 tacgcacgct gcaatatggt gaagcgctgg atgatctctt aaccgcgatt aaaaataagc
     7681 aaaatgattt gcaagcgatg caccataaat atgtgccgat cgcagtgaag atcgcgccgg
     7741 atctttctga agaagaattg atccaggttg ccgatagttt agttcgccat aatattgatg
     7801 gcgttattgc aaccaatacc acactcgatc gttctcttgt tcagggaatg aaaaattgcg
     7861 atcaaaccgg tggcttaagt ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc
     7921 gcttgtcact ggaattaaac ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg
     7981 ttatcgctgc gcgtgaaaag attgctgcgg gtgcctcact ggtgcaaatt tattctggtt
     8041 ttatttttaa aggtccgccg ctgattaaag aaatcgttac ccatatctaa ttatttcttc
     8101 gaattcctct ttataagata atcagggctt tattttcagc cctggttgtt ttatattcat
     8161 cgctgttgct tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcgaca
     8221 atggattgtg ttgctgcggt tttatagggt aggggagagg cagatgcgaa ttaaaccaga
     8281 cgataactgg cgttggtatt acgatgaaga gcacgatcga atgatgctcg atttagccaa
     8341 tggtatgcta tttcgctcac gttttgcgcg caagatgttg actccagatg ccttttctcc
     8401 cgcaggcttt tgcgttgacg acgccgcgct ctatttctct tttgaagaaa agtgccgcga
     8461 ttttaattta tctaaagaac aaaaagccga actggtgctg aatgcactgg tagcaattcg
     8521 ctatctcaaa ccgcaaatgc cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt
     8581 gcccatgccg ggcgatgcgg cctgcgtttg gttgagcgat actcacgagc aggtcaattt
     8641 gctggtcgtt gaatctggcg aaaatgccgc actatgcctg ctggcacaac cctgcgttgt
     8701 tattgcgggt cgcgcgatgc agttgggcga cgccattaaa atcatgaacg acaggctgaa
     8761 accgcaggtt aatgttgaca gcttcagcct cgaacaggct gtctaacgcg ccaacttaag
     8821 tgcagtcttc ggtacacagc tacagcaaag aatggtgcca tcatcgccca ttgctgattt
     8881 tttcagcggc gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc
     8941 gcggcaagaa taagggatac gaattccctg attttctaat tgctccagca acacctgttg
     9001 gttatttcca cgaaatgcct gtccctgcca gtcaatatct acatttgcgt ccggttgttg
     9061 cgtgatgttg gcggtatcat cagcggcagc tgcgccgtaa atttttgccg gagccgttgc
     9121 cagaatttcc acctcatcgc caacgcgaat cacgccgcta ttacgggcaa ttaaattctg
     9181 accaaaatcg acatcgccgt tatcctgggc agtgcggaaa gattgcaatg tttttaatgg
     9241 ttcgcctgcc ggatgttttt gccctttttc tgggctgacg gtggtgaaaa tacagcggct
     9301 acaaggttta accacatcaa acaccacatc accaatgcga atcactttcc agctatcttc
     9361 ttcccaggct gacgcgccgg aaaccaccag attggggcgg aattgctcca tttttacact
     9421 ggccggacaa cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata
     9481 gccatcagca aatgacaggg gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg
     9541 ccccacccag cgtaattgca cttcgcggga gaaaaatcca cttagccatt tgttgatcgc
     9601 gtctggcgca attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt
     9661 ggcgaaatca gcaaaacgaa catatgcgct actgccatct ggtgcggtga gatgcaagcc
     9721 atcatgcaca ggtgaaggag taaaccgtac catctgggga aactggcgag cggtaataaa
     9781 cgtaccgtca ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatcagc
     9841 cagagcatgt gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt
     9901 taatgtcgcc acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc
     9961 gcttattagc tataatgcgc aacaattttc ttagcgcctg tcccattagg ctattttatt
    10021 tgtcagtttg gccctgggtt gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc
    10081 tccgcgctgt ccatgtccag actgactgca acaattacgc ctactgcggt aggctcttag
    10141 agtaaaagtg acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg
    10201 ttaaaaactg aactggaaaa cctgggggcc gttgaatgcc aggtggttca gggtggggtc
    10261 catttcaagg gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg
    10321 cgtattatgt tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt
    10381 gttcaggcga tcaactggac agagatgttt aatcctggcg cgaccttcgc tgtccacttc
    10441 agtggtttga atgacaccat ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg
    10501 atcgtcgatg ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg
    10561 gatatccgcg ttaacgtctg gctgcataaa gaaaccgcca gtatcgctct tgatctgagt
    10621 ggtgatggtt tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa
    10681 accctggcag ccgctattgt gatgcgatcc ggctggcagc caggaacacc gctgctcgat
    10741 ccgatgtgtg gttccggtac gttgctgatt gaagcggcga tgctggcgac cgatcgcgca
    10801 ccaggcttgc accgtgggcg ttggggcttt agcggctggg cgcagcatga tgaagctatc
    10861 tggcaggaag tgaaagcgga agcgcaaact cgcgcccgta aaggcctggc tgagtatagc
    10921 tctcattttt acggttcgga cagcgacgca cgggtgattc aacgtgcacg cactaacgcc
    10981 cgtcttgcgg ggattggtga actgatcacc tttgaggtga aagatgtcgc gcaactgacc
    11041 aatccgctgc cgaaagggcc gtacggcaca gtgttgagca acccgccata cggggaacgt
    11101 ctggacagcg aaccggcgct gattgcgctg catagcctgc tgggtcggat catgaaaaac
    11161 cagtttggtg gctggaatct ctctttgttt agtgcctcgc cggatctgct aagctgcttg
    11221 cagctgcgtg cagacaaaca gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa
    11281 aattaccacg ttgccgaaag cactccagac agcaaaccgg cgatggtagc ggaagactac
    11341 gccaaccgcc tgcgtaagaa cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt
    11401 gaatgttacc gcctgtatga cgccgatcta ccggaatata acgttgccgt tgaccgttat
    11461 gccgactggg tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg
    11521 cgtcagcgtc tgttcgatat tatcgctgca accatttcgg tactggggat tgcgccaaac
    11581 aaactggtgc tgaaaacccg tgaacgccag aagggcaaaa atcagtacca gaaactgggc
    11641 gagaagggcg agtttcttga agttaccgaa tataacgctc acttgttggt gaacctgacg
    11701 gattacctcg ataccggtct gtttctcgat caccgtatcg cccgtcgtat gctcggtcag
    11761 atgagcaaag gcaaagattt cctcaacctg ttctcttata ccggcagcgc caccgtgcat
    11821 gcgggattag gcggtgcacg cagcaccacc accgtggata tgtcgcgtac ttatctggag
    11881 tgggcagaac gcaacctgcg tttgaatggc ctgaccgggc gtgcgcatcg cctgattcag
    11941 gccgattgcc tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ctttatcgat
    12001 ccgccaacct tctctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat
    12061 ctggcgctga tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg
    12121 aacaataaac gtggcttccg tatggatctc gacggcctgg caaaactggg actgaaagca
    12181 caagaaatta cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca gatccacaac
    12241 tgctggctga ttaccgcagc ctaaaggaat agtaatgtca ttaatcagta tgcatggcgc
    12301 atggctgtcg ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa
    12361 cgaacgtgtt tgtctggtgg gccgcaacgg cgcgggcaaa tcgacgttaa tgaaaatcct
    12421 caaccgtgaa caagggctgg atgacggtcg cattatttac gagcaagatt tgattgtagc
    12481 tcgtctgcaa caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga
    12541 aggcattgaa gaacaagcgg aatatctgaa acgctatcac gatatttcgc gcctggtgat
    12601 gaacgacccg agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca
    12661 ccacaacctg tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga
    12721 tcctaacgtt gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg
    12781 cgcgctggtg agtaatccgc gcgtgctgtt gcttgatgaa ccgacaaacc acctggatat
    12841 tgaaaccatc gactggctgg aagggttttt gaaaactttc aacgggacga ttattttcat
    12901 ctcccacgac cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg
    12961 caagctggtg acctatccag ggaattacga ccagtacctg ctggaaaaag aagaagccct
    13021 gcgcgtggaa gaattacaaa atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg
    13081 gatccgccag gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa
    13141 agcgatgcgt cgcgagcgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt
    13201 ggaagaggcc agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt
    13261 tgacggtaag caactggtga aagatttttc ggcccaggtt ctacgtggcg acaaaattgc
    13321 cctgattggt ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct
    13381 tcaagcggac agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca
    13441 gcaccgcgcg gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca
    13501 agaggtgatg gttaacggca agccacgcca cgtattgggc tatttgcagg actttctgtt
    13561 ccatccgaaa cgggcgatga cgccggtacg tgcgctttct ggcggtgagc ggaaccgctt
    13621 gctgctggcg cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa
    13681 cgatcttgat gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac
    13741 ggtattgctg gttagccacg atcgtcagtt tgtcgataac accgttacag aatgttggat
    13801 cttcgaaggc ggcggtaaaa ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca
    13861 gcaagagcag tatgtggcgc tcaaacagcc tgcggtgaaa aaaaccgaag aagccgccgc
    13921 ggcaaaagca gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga
    13981 actggagcag ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac
    14041 gcaggtggcg gatgcttcct tcttcagtca gccacatgag cagacacaaa aagtgctcgc
    14101 cgatatggct gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga
    14161 agcgttaaaa aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac
    14221 ggtaaaattg cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat
    14281 ctgtaaaata gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca
    14341 catcctgtgc tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa
    14401 agcggcatgt ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg
    14461 tcccaccgcc tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt
    14521 tgtgaatatg aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt
    14581 gcttttttct gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt
    14641 tcccgcgttt tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg
    14701 tttaaaagag caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat
    14761 tttcctcgct ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt
    14821 aggcagcagc tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg
    14881 cgttgatcgt cgctggttat gggacgacat cgccccgatg ccagaactgc gccagccgct
    14941 aaaaccaggc gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat
    15001 ccttcctgct gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag
    15061 aaacagcctg cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc
    15121 taatattttg cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct
    15181 cgctggggtc attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct
    15241 ggccagtatt atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc
    15301 caaagggcat ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt
    15361 tgtaggccgc tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg
    15421 tatgggaggt ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt
    15481 cataatgaca atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc
    15541 tgaatcagag catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc
    15601 agaaagtgaa gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg
    15661 cctgggttct tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga
    15721 atgcggaagg aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg
    15781 tggttgaaag cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga
    15841 attccggtat ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga
    15901 ttggtcgcga agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc
    15961 agccaggcgc gaaaggcagc aaaatggata aatacgattt gctggactcg ccaccgttgg
    16021 ccccgcctga tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct
    16081 cgccaggaga tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct
    16141 tcgatacaca aaaacgcaat atcagctatc aactgttcat caatgcacct tatgaccgac
    16201 tggtgaccaa caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag
    16261 cagggatgcg tgtggagatg ggctcattga caacgctgct gagtggcggt gtcagctttg
    16321 atgtgccgga aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt
    16381 atgatgatca gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt
    16441 tctttaaaga ttcggtacgc ggtctgcaac cgggagctcc ggtagagttc cggggtattc
    16501 gcctgggtac cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg
    16561 atgattaccg tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg
    16621 gcgaaaatgc ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat
    16681 cgctgaaaac cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa
    16741 atacgcctgc aataaccggt attcgtgaat ttaatggtta tcagattatc ccgaccgtta
    16801 gcggcggcct ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac
    16861 tgccattgaa tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa
    16921 tgaaaaacct gcaaacgacg ctggatagca tgaacaagat cctcgctagc cagtcgatgc
    16981 agcagttgcc gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct
    17041 tccagcctgg ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg
    17101 tgttgcgaga actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat
    17161 ttgaagcgaa ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct
    17221 agtgacgatt gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta
    17281 ttaccagtta cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct
    17341 gttatgggta gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca
    17401 aaccagtgat gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca
    17461 acagttgcgc aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc
    17521 ctcccagcct ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg
    17581 tcgctatgat ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact
    17641 gatcaaacgt ccgttccgtc tggaaggatt gcaaactcag gatggttacg atgagatggt
    17701 taaagtgctg gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg
    17761 tctaccttaa ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt
    17821 ttttttgtct ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca
    17881 tgcccgtgta gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg
    17941 attcgcggta ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca
    18001 gaaaccagta tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc
    18061 acatcaacgt ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca
    18121 gacgctgaat caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt
    18181 agtaatggcc tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt
    18241 ttgatactct gtctgattat aatcagaagg cagacgtatc ctggaacaga ccgactttca
    18301 ggtcgctggc ggtatagatc agacgaccat caaccagcac ttcgccatcc gccaggccca
    18361 taatcagacg acggttaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg
    18421 tcggcagtac ctgaccagtg aatttcactt cgccaacgcc cagtgcgcgg cctttacctt
    18481 cgccgcccag ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc
    18541 atcccggcat aaccggatcg ccaataaagt ggcatccgaa gaaccacaga tccggattga
    18601 tatccagttc tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga
    18661 ccacacggtc catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca
    18721 gttcaccgcg accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca
    18781 tgttctctgt aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa
    18841 caagtccgat cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg
    18901 acgtccttcc tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt
    18961 ttggccttcg ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc
    19021 tactgcccag atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact
    19081 taaatggcga acgttagctg tggggataat gacaccttgt ttcccggtta actcacgttg
    19141 ctggcaaata gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg
    19201 accgaactga tcgactgaac ctgtgatagc gatactctga ttcaccggca catcggcgag
    19261 ggcgcttatc agggcgcaga gttcagccat cgaggcacta tctccatcaa cttcactgta
    19321 tgactgctca aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga
    19381 catcaggaac gcttgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc
    19441 tttgcgttcg atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga
    19501 aggttcgcca aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg
    19561 cccgatgcgt tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat
    19621 acgttcagcg aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc
    19681 gccggagaag gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca
    19741 ctgcgggcta agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag
    19801 tatcggccag gcatccgctc ccggtgcagg taagtgatta tgtctggcgg taaatgtcac
    19861 ccagcgacac cactgggtta ctgactccgc atcgacaatc tgcagagtat cttcaaattc
    19921 gctataaata gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc
    19981 gcgttcgcct accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag
    20041 agggcgcgac tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag
    20101 ccgcatccac agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc
    20161 atttgcctga tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca
    20221 gccaaagagt tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa
    20281 attatcatct gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg
    20341 accgcccacc agttggcctg catcgctttg tagcgtacgc gcggcattgg caataagatt
    20401 cagatactca gactcttccg gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg
    20461 cagcaactgc tccagcgcaa attgcagccg cggttgagta tcactgaata aaggatcgtt
    20521 ttcgtcaatc aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag
    20581 gtcacgccat gcaagtttcg taatggtcaa agttgatgtt tttttgtctg ttgtcaaagc
    20641 cgcgattata ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc
    20701 atgggcgtta aagctcacag gatggagatt cttttcttca ctggcctaaa aagctgatat
    20761 tctgtaaaga gttacacggt aacattgaga tcgctatgaa atatcaacaa cttgaaaatc
    20821 ttgaaagcgg ttggaaatgg aagtatctgg ttaagaagca tcgcgaaggg gagttaatca
    20881 cccgttacat agaagccagt gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa
    20941 atgaacccgt gctggtaaat ggctggattg acaagcatat gaatccggaa ctggtcaatc
    21001 gcatgaagca aaccattcgg gcaagacgaa agcgccattt taatgcggaa caccagcata
    21061 cgcgcaaaaa atcgatcgat ctggaattta ttgtctggca acgtctggct ggtcttgcgc
    21121 agcgtcgcgg taaaacgctt tctgaaacga ttgttcagct gattgaagat gcggaaaaca
    21181 aagagaaata cgcgaataaa atgtcttctc tgaagcagga tctgcaggca ttgctgggta
    21241 aggaataact gacgaaagtc agttcaattt actaaaggca aaaaaaaccc cgcagcagcg
    21301 gggtttttct accagacgag aacttaagcc tgcggctgag ttacaacgtc tttgatacct
    21361 ttaacttcga tctctacgcg acgatccgga gccaggcagt cgatcagtgc agcacgctgt
    21421 ttcacgttgt cacaggtgtt gccagtaacc gggttggatt cgcccatacc acgtgcggag
    21481 atcttgtctg ccgggatacc tttggagatc aggtaatcaa caacagactg agcacggcgc
    21541 tcggacagac cctggttgta agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact
    21601 acggaaccgt ctttcggatc caggttgctc agctggctgt acagctgatc cagagcagcc
    21661 tgaccttccg gtttcagggt tgctttgttg aagttgaaca gaacgtcaga cttcagagtg
    21721 aagtgcttgg tctgtacttc cggtgccgga gctggagccg gagcaactac tggagctgct
    21781 tcgccctgac cgaaacggta ggaaacaccc aggctcagca tgccgttgtc cggacgagtg
    21841 ccgatggtgt gtgcgtcacc gatgttgttg gtccactggt attccagacg ggtagcgatt
    21901 tcaggagtga tcgcgtactc aacaccacct gcgaaaaccg gagaaacgcc agtgtcgtgg
    21961 tctttaaagg atgcgccacc aggtacgtta gccttggtgt ctgcacgcca taccatacca
    22021 cccagacgag tgtatacgtc cagatcgtca gtgattgggt aacccagttt agcagtcagt
    22081 tgaacgccct gagctttgta tgcaccgttg atgttgtcgc ctttgtacgg catacgacct
    22141 aaccagtcgt aacccatttc aaaaccaaca tacgggttaa cctggtaacc accaaaagca
    22201 cctgcaccca gttggttttc gtgggtcgga ccattgttag gaataaaacc agtgtcatgg
    22261 tactgggacc agcccagttt agcaccagtg taccaggtgt tatctttcgg agcggcctgc
    22321 gctacggtag cgaaaccagc cagtgccact gcaatcgcga tagctgtctt tttcattttt
    22381 tgcgcctcgt tatcatccaa aatacgccat gaatatctcc aacgagataa cacggttaaa
    22441 tccttcaccg ggggatctgc tcaatattaa ctctaccgat atcttcggct tatgccgagc
    22501 acccctggcg atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga actccgtcag
    22561 gcatatgaaa aaaaagtctt gtataaggta cgtttaatct tttttgtcag cgacaattta
    22621 cagaaaaaaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt agtcacgact
    22681 gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt acttaatgat
    22741 acaaattaga gtgaattttt agcccggaaa gttgtctcgt tgcgtgagag gatgcgctta
    22801 ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat
    22861 gctcttcttc agtcaaatca tctgccaacc aaccgatcac cacactgtaa ttgcccgtgc
    22921 gtaaagcgcg aaccattgac tccacagtgt ggcaaggggg gagctggcta atctgcatta
    22981 ctttcgttaa gggtagccca gatgcctgaa cccattcccg actcagtttt tgttgcggtg
    23041 ttaaccagag ttgccagcgc gattgctgac cgagttgctg taacaatggc aacagtagaa
    23101 gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc ccggctgtag
    23161 tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac gaagaacgat
    23221 gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt gatttactgt atggatgtac
    23281 agtacatcca gtgacaacaa agatcaaccc tattttcgga aagagcctcg caaattttgt
    23341 cgttggtgac gggaaaacat aaattaatct tgcccctaaa gaataagttg cctattttcg
    23401 tatttaacgg atccgttaat gtgaatcatt cttttatgtt atgattttaa aaggaatttt
    23461 atgaaaagcc tctcctataa gcggatctat aaatcacaag aatacctggc aacgttgggc
    23521 acaattgaat accgatcatt gtttggcagt tacagcctga ccgttgatga cacggtgttt
    23581 gcgatggttt ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc acagtactgt
    23641 gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt taccctcaat
    23701 tactatcggg ttgatgaaag tctatggcga aatcaactga agctggtgcg tctgtcgaaa
    23761 tattctctcg atgcagcgct gaaagagaaa agcacgcgca atacccggga aagactgaaa
    23821 gatttgccca atatgtcttt tcatctggaa gcgattctcg gggaggtggg gattaaggat
    23881 gtacgggcgt tacgtatact tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac
    23941 agtctggtga cagaaaagat tctgtttatg cttgaaggtg ccattatcgg cattcatgaa
    24001 gctgcgctcc cggtggcacg ccgccaggag cttgcagaat gggctgactc tcttacgccg
    24061 aaacaggagt ttcctgcgga acttgagtaa tctggcgttg cagacgacca atctcaggca
    24121 gtaatgcaat caataatcca acttgttgta cgaccagagg ttctttgctg tctgcccgtg
    24181 gttcaagttg ctgcatccgc tgtttcaggc tagctaatgc ctcattgacg cgttcttcat
    24241 cagcaggttg atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca agaaacgcca
    24301 gaatttcagg attagttaac tgctcccggt gagcaccgag ggctgagata tagctggtaa
    24361 acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg aatttgcggg gtaacgttcg
    24421 gctcgctgga catatttgat accaccgacg ccagctcagc atcacggttg tgtgcatcgc
    24481 ggcgggcaat acgatacgcc agacggttat cacgcccctg atggtattgc tccagtatgg
    24541 catcgagata ccgacagttg gcctctgtgg cgcgttcgag catgcgcggc agattgcgaa
    24601 actgccagtc aggccagatg tagctcactg ccgcccacgc aatggcacaa ccaatcagcg
    24661 tatcgattac gcgaggtaac gctacttcaa aaccttcacc cagtaagtta aaacacagta
    24721 gcaccaaaag tgtgatgaac atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa
    24781 agagcacgcc ggtaataacc agcagcacca gctgcccttc cagtgatggc acaaaccaca
    24841 gcacaggaat gccaatggcg atacctacca gcgtaccaat aatccttaac ttcaggcggt
    24901 ggcgcgtggc gttatagttt ggctggcaga caaacaaact tgtcagcaag atccaatacc
    24961 cgtgatgcat tccggttatc tgaatgatgg cgtagccgaa gcacaacacc agcgacattc
    25021 ttaccgcatg acggaagagg gcggattccg gcgtgaagtg acggctaaga cgcagccaga
    25081 tatcactcaa cccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta ttatggggta
    25141 gtgcctgggc ctgttctgat tcaattgttg ccagttgggc atcaatggcg cgtaaattgt
    25201 tcagcaaaaa tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg cgcatccgct
    25261 ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt tgataaggct
    25321 gacgcaacaa aatacagcgt gacagttgct ggcacgcctg gccctgcatc gacatcagcc
    25381 gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa
    25441 tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc
    25501 gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt cagcagcgag agtttcgtct
    25561 gattcaatgt cgccatcagc agaccgttgg cgagagccaa atcgtacagc ggtgcctggc
    25621 tttgatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt
    25681 gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc agatgaccaa
    25741 taagtgtcag gacgttgtac cagacggcac cggccagcag atacatcggc tgctgatacc
    25801 agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat gcaccgaagg
    25861 caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg gtagagagcg
    25921 ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag gccgaggcga
    25981 taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt cggtcatcga
    26041 gatcggtcag cgccgctgcc accatcccca gcgttagcgg aatcgtcagt tttacatcac
    26101 ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc gcgtaataca
    26161 gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag gccatattag
    26221 tggattattc aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg
    26281 ataccacgcg acgaccaacc ggccatagtg caatagcggc aattttaaag ttcgcaatgc
    26341 cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc
    26401 ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg ccagtattca
    26461 gcagcacatt tttgccagcc gggttcagtt catcgacatg aatagcttca ttgccataag
    26521 gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagcggtaag gtaaaaatca
    26581 gcacaatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat ccgccaagca
    26641 caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg gttttcagta
    26701 atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac ggttacactg
    26761 ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac aacgcttgga
    26821 aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc
    26881 tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat tcactgtaag
    26941 cgcggtctgg tatggggcga gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg
    27001 cacggcaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc tcactggcgg
    27061 cggtggagtg gcgagatgag cgaaattgcg tctggtgttt tacgccagca actggatttg
    27121 attgccacgc gcactgggga aaataaatgg ctgacgcgtg agcaaacctc tggcgtgcag
    27181 caacaaatcc gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc
    27241 gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagatat tgaaagcgct
    27301 cggttgcagc ataaccaggc gtataccgaa gccatgctta ccgagtatgc ggattttttc
    27361 cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt taatggcgag
    27421 cattctctgt tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt
    27481 gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt gctggcgttt
    27541 ggtcgcaaag ccgctgaaga gatggacgag cggattcgcg aacggctaca taccgaagac
    27601 attaccgcac gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa
    27661 gttccgatag tcagcaaact ggaaaatgat accgctgccc gtcatgaact ctttattgct
    27721 gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg
    27781 acggaagaaa tgcagtggtc agtgccagaa ggtaacttct gggatgatga aaaattacag
    27841 cgtcgccttg cctctcgcct cgatcgctgg gtaagtctga tgcggatgca cggtggtgca
    27901 caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag taaacgtatc
    27961 aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc
    28021 gatttttcgg gccttattca tcaggcgatt gtgattctgg agaaaggtcg ctttatcagc
    28081 ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg ggcagcgttg
    28141 ttagcggcat tacgcaagca aaacagtcag acgacgttgt tcgccgttgg tgatgactgg
    28201 caggcgattt accgattcag cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac
    28261 tttggtgaag gcgatcgctg tgatttagac acgacttacc gttttaacag tcgtatcggt
    28321 gaggtggcaa accggtttat tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc
    28381 ttaaccaatg gagacaaaaa agccgtcacg ttattggatg agagtcaact tgacgctttg
    28441 ctggataagc tctctggtta tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac
    28501 catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc
    28561 gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcat catcgttggc
    28621 ttgcaggagg gaagtgatgg ttttccggct gcggcgcggg agtcgattat ggaagaggcg
    28681 ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat gtacgtggcg
    28741 ctgacccggg cacgccatcg ggtatgggca ctgtttaaca aagagaatcc ctctcccttt
    28801 gtggaaatac tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc
    28861 gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga taatcgggga
    28921 tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat tatgaagtct gccgttgcca
    28981 cgttggtggc gaccggaatg ttccataccg tcgccagacg cagcaaggct ttcacgtcag
    29041 gatcgtgcgg cacggcattt agtggatccc agaagaaaat caatacatca attttccctt
    29101 ctgagatcaa tgcgccaacc tgctggtcac cccccattgg gccactcaac atcgcgttga
    29161 cgttcatgcc ggttgcacgg gaaatcaagt taccggtagt gcctgttgca tacagtacgt
    29221 gttgttccag taacggttga tgccgttcca cccagctcat cagcatttgt ttgcagtgat
    29281 cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc agttccataa
    29341 tgtacatccg tagttaactt tcctacagat tactgtaagc acttatcgct gcaagataaa
    29401 gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt tttccgccca
    29461 ctgcaggaaa gtatttcgcg tttgtgggtc agccagttta aaccaatact tcagccgttg
    29521 ttctgtgagc acctgagact gcggtggaat agcatccagc tcggaaacac ccgaaagcag
    29581 cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt
    29641 gttatagcgt tcaacctcga cctcataatc aatcgttttt gccgttgagg taatggcgag
    29701 aatatccagc tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc
    29761 ggcatcaaaa tggttagctt cccgctcatt ctccaggcga ggcaggcgaa aattgacctg
    29821 gttgatgagc tgggtattaa aactgaccac cagcggtggg gagatataca gccgttcttc
    29881 actattggaa agatgaatcg tcttctcaac gcgaaacact aactgatgcg gtccattatc
    29941 cagttcaatg ctgtccgcac cgcgcagcag agagctggaa accttcttgc cgtcgagtac
    30001 cagaagatcg acatcggttg aaagccgcag cgtggtggca aaaacggata ccggcaaaca
    30061 taatgcaatc aaggtggtca cgatgccggt tttcataggt agctcctgtc aaaagaccgc
    30121 ccgaataata atgtgtcaaa atttttcttt aatcatactt actaacatat agacatattt
    30181 cccgcttttg ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta
    30241 cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag gcattttaac
    30301 gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc ccagctatcg
    30361 cgtgatgaaa tatctactcg accaggggta tcacgtcatt ccggtttcgc caaaagttgc
    30421 cggcaaaacg ctgctgggcc agaaggggta tggcacgctg gcagacgttc cggaaaaagt
    30481 cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcac aggaagctat
    30541 tgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc aagcggcagt
    30601 actggcacgg gatgccgggt taaatgtggt gatggatcgc tgcccggcta ttgagatccc
    30661 tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg gggatttgtt
    30721 cagggattag ttacgcagac gcggggcctg gagttgtttg cggatggtct gcgccagctc
    30781 atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct gggcttcagc
    30841 caggtaggta tgaaccggta ggccgttatc gtcctccatc actacgtgat accacggagc
    30901 ggcgcgaagc tcgtcattca ccgccagctc atcaggcgac ggttcagaga gcgaataaac
    30961 cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga cctgctggcc
    31021 gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttacct ttgatataag
    31081 ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca gatatagccc
    31141 ttccggatag gtagcgatca ccggatgatc ggctgcctga cggaactgct ctataaattg
    31201 tacatcacga ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt
    31261 catcagaccg gaacaggaga aagtcaggag aataccgcct tcattcagca gctgaatcgc
    31321 cagcatgttg atgtctttat aaccacgaca cgcgcccatc aactggcttt tattctcaac
    31381 aaacttcggc gggtccatca cgataacgtc aaatttttca ccgcgatcgc gataagtacg
    31441 cagcaattta aagacatcat cacggacaaa ctcagccttg ctcagatcca gtttgttcag
    31501 ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc tgacaacctg
    31561 gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg gtataggaga aacagttcag
    31621 cacacgttta ttttcaacgt agcggcgggt agccaggcgg ctatcacgct ggtccaggta
    31681 gtagcccgtt ttgtgtccgt gctgaatatc caccagcagt ttcattccgt gttcttcaat
    31741 cggcagcagg gcaggtggca actcgccggt gacggggccc tgggtcagct ccattccttc
    31801 ttttttacgt accgcgacgt cgctgcgatc gtaaatcgaa cattccgggt acagcgtttg
    31861 cagggcacta attaatgccg cgcgctgata ttctgcccca gcactcagca gttgcagcac
    31921 cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt ctccggcgat
    31981 taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt tttgtgcttg
    32041 ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa acgtccagac
    32101 gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt gctaaccatt ttccctgatg
    32161 atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg caacggcccc
    32221 ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg ctaacactaa
    32281 acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt
    32341 tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt cgaaagtctg
    32401 cataattgcc tgggtttacg ggcgagttca gggcgtagga tttcgctaca ccacacagta
    32461 cgaagcgaaa agactgggtt taaccgggta cgccaaaaat cttgacgatg gcagcgtgga
    32521 agtggttgct tgcggtgaag aagggcaggt ggaaaaatta atgcagtggt taaagagtgg
    32581 cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc cctcggggga
    32641 attaaccgat tttcgtatcc gttaaataca ttttaccggt tttggcaggc cagcaatttt
    32701 ggtggcttgc ttagccggac ctttcggaaa cagtcggtat aaatagcggc tgttaccttt
    32761 ttcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg gagaagtatt
    32821 gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttccg gtgacagcga
    32881 aatcccttcg ttctctgcaa tcaccacagc cagcggctca ctccactggc tgctttcttt
    32941 gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca taattattac
    33001 taccttaatc agactggcgg cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta
    33061 tgaaagtgtt agcgggtgag attaatcgcg gctagcgaag cccagaatgc tcagcaggct
    33121 gacgaagatg ttgtacagcg aaacatacag gctaaccgtg gcacgaatat agttcgtctc
    33181 accgccatga atgatgttgc tggtttcaaa caagatagcg ccagaggaga tcagaatgaa
    33241 gaccgcgctg atcgccagat gcagagcagg cagctgcagg aagatattcg caaccatacc
    33301 aatcagcacc accacaatac ccgccatcag cataccgccg aggaacgaca tatctttgcg
    33361 ggtggtcagc acatatgcag agcagcagaa gaacactaac gccgttccgc ccagtgccat
    33421 agcgattacg tcacccattc cggcagacag ataggtgttc agaataggtc cgaggatata
    33481 acccagaaaa ccggtaaagg cgaatgcgga gataatcccg gtcggcttat tcgccgtttt
    33541 ataggtcagg aacatcaaac catacatacc caccagcgtc agaatcagac ccggagatgg
    33601 cagcatcagc accgtactgg cagttgcggt aatcgccgaa aaggccagcg tcaggctcag
    33661 cagaaaatag gtattacgca gcaccttatg ggtgctaagc agtgatgtac ggtcatgtga
    33721 agaactaaca atacgatcca ttgagtcact ctctatgaca gatgtaatta attaagcagc
    33781 ataatgataa tgcgtaaggg cacccagaag ttttacccat ctttacgcat ttgatctgga
    33841 acaggtttaa cagcggatta tcaggtcatt aagcaaatat aacgccctga gaatttcgac
    33901 aggcaaaaga aaaaggggtt agcatttagc taacccctta tcttatttgg cggaagcgca
    33961 gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccgaag aaaaacaaat
    34021 aaaaatcagt gcaatacgtg aaattgctga aaagttaatc aaatttaaca ccaagaaaaa
    34081 tcaaggtatt aggaagttgc tcatagctag tataccaagt ctttttgcca gaaatttttc
    34141 tggattgtct acttgctaac cacgagcggc aaatccagtg ttggtgtgat ttttgtttta
    34201 cgatcgtaaa tcaacacctg attttctgtt ttatgcccgc tgaaaatttg tttgtcgcga
    34261 ctgctgcctt cgtaatctga gatccccttg gcttttatgt cgtgaaaatt gcacccgaac
    34321 ggaacgccaa ctttttgctc ggctgcgcgt ttagcctgat tccaccagtt attcagcgtt
    34381 ttagctatga ctttcccgcc tttggttgta ttgatcacat attcgcatgt acaggaagat
    34441 acatttcggg ctaactggat cgctgtacgt aatcgcggtg accattcctt gatttgtttg
    34501 gtgccggttt tgttttgctc aatgtagatc cctttatcca taatatcctg ccatttcaac
    34561 tcaagcacat caccgagtct tgccgcacag agataggaaa tctccattgc aatgcgtaac
    34621 tgtggaattg cttccgcata tatcgccgca tactgttcat cggtgatgta aacagtgcgg
    34681 gctttaagag agaattttct gactcctttg catgggttat tcttcacata cccacgctca
    34741 tatccccacc cgtatactcg actcaggctt gccagttcat gatttgcctg ggtcttgctc
    34801 tcaagccctc gtttatccat gaaaattctt acctgctcag ttttgacatt atcagcaagc
    34861 acttttccga ataccatcag caacgccttc tgatgttgtc gataatcttt ttgggttcgg
    34921 ggggccagtt ctgtaaatgc aggggagtcc ataaacatgt gccataattt agctacggtc
    34981 attatgttgt ggagttttgc tttttccagt tcataatttt gccagacttt agctacgctg
    35041 gttttccgca ctcttcctag ccctatactt cttgtacttc cttcgggttt ccacacgtaa
    35101 ctgtaaccat tcgatctaac ccgcggtggc agtacattat cttttttatt ttttcttggt
    35161 cttcccattg ttcagcgcct caaaatcggg ttcagcagaa aacagttcag atgtttttgg
    35221 catcgttgtc agaccgtgtg gaatatccct gcggagaact atgggttcgt ttttagggcc
    35281 gattacaaat gggatgccgt gcagtcttaa ctggtgttgc tgttttgtgt atcgctcgta
    35341 tttcgtgatc tctttaatct ctgctggaga tagagttaat tcgtacatgt ggtcacgtgc
    35401 ctttacagca tgaccgccgc cactatagct ggtgggcggc gatatgggtt gaacattaaa
    35461 aatcagcctg actcgggagc agtttttgcc agatggctga aacgtatttc gcctggtaac
    35521 gtgcatcgtt cagagcgtta tggcgttcac cttcgaatgg aatagtcgtt cttgcgtcga
    35581 aatccatcac cagtcccaga gcaaccatcg ttcttacatc gcgatcattg gtgtaacgcc
    35641 acgggcaggg gatcccctgc cgttcatatg aacggcgtaa aatcacgttg tcgaaagttg
    35701 caccgtt
//
LOCUS       DAZFZC010000043        35288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFZC010000043 DAZFZC010000000
VERSION     DAZFZC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.043"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..1485)
                     /gene="xylB"
                     /locus_tag="ACHKXP_002477"
     CDS             complement(31..1485)
                     /gene="xylB"
                     /locus_tag="ACHKXP_002477"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO7647460.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1557..2879)
                     /gene="xylA"
                     /locus_tag="ACHKXP_002478"
     CDS             complement(1557..2879)
                     /gene="xylA"
                     /locus_tag="ACHKXP_002478"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO7647461.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHHLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3245..4237
                     /gene="xylF"
                     /locus_tag="ACHKXP_002479"
     CDS             3245..4237
                     /gene="xylF"
                     /locus_tag="ACHKXP_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7647462.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4315..5856
                     /gene="xylG"
                     /locus_tag="ACHKXP_002480"
     CDS             4315..5856
                     /gene="xylG"
                     /locus_tag="ACHKXP_002480"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO7647463.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVPCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5834..7015
                     /gene="xylH"
                     /locus_tag="ACHKXP_002481"
     CDS             5834..7015
                     /gene="xylH"
                     /locus_tag="ACHKXP_002481"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO7647464.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7093..8271
                     /gene="xylR"
                     /locus_tag="ACHKXP_002482"
     CDS             7093..8271
                     /gene="xylR"
                     /locus_tag="ACHKXP_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO7647465.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8467..9291)
                     /gene="bax"
                     /locus_tag="ACHKXP_002483"
     CDS             complement(8467..9291)
                     /gene="bax"
                     /locus_tag="ACHKXP_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO7647466.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9611..11641
                     /gene="malS"
                     /locus_tag="ACHKXP_002484"
     CDS             9611..11641
                     /gene="malS"
                     /locus_tag="ACHKXP_002484"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7647467.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIM
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKSLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKMDTHAKAIDGYTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLLLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            11819..13072
                     /gene="avtA"
                     /locus_tag="ACHKXP_002485"
     CDS             11819..13072
                     /gene="avtA"
                     /locus_tag="ACHKXP_002485"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO7647468.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13223..13696)
                     /gene="ysaA"
                     /locus_tag="ACHKXP_002486"
     CDS             complement(13223..13696)
                     /gene="ysaA"
                     /locus_tag="ACHKXP_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7647469.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREQGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13798..14646)
                     /gene="yiaJ"
                     /locus_tag="ACHKXP_002487"
     CDS             complement(13798..14646)
                     /gene="yiaJ"
                     /locus_tag="ACHKXP_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO7647470.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14847..15845
                     /gene="yiaK"
                     /locus_tag="ACHKXP_002488"
     CDS             14847..15845
                     /gene="yiaK"
                     /locus_tag="ACHKXP_002488"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO7647471.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15857..16324
                     /gene="yiaL"
                     /locus_tag="ACHKXP_002489"
     CDS             15857..16324
                     /gene="yiaL"
                     /locus_tag="ACHKXP_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO7647472.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYTQIIDLTTREAVVNRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16442..16915
                     /gene="yiaM"
                     /locus_tag="ACHKXP_002490"
     CDS             16442..16915
                     /gene="yiaM"
                     /locus_tag="ACHKXP_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO7647473.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16918..18195
                     /gene="yiaN"
                     /locus_tag="ACHKXP_002491"
     CDS             16918..18195
                     /gene="yiaN"
                     /locus_tag="ACHKXP_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO7647474.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18208..19194
                     /gene="yiaO"
                     /locus_tag="ACHKXP_002492"
     CDS             18208..19194
                     /gene="yiaO"
                     /locus_tag="ACHKXP_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO7647475.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19198..20694
                     /gene="lyxK"
                     /locus_tag="ACHKXP_002493"
     CDS             19198..20694
                     /gene="lyxK"
                     /locus_tag="ACHKXP_002493"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO7647476.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            20691..21353
                     /gene="ulaD"
                     /locus_tag="ACHKXP_002494"
     CDS             20691..21353
                     /gene="ulaD"
                     /locus_tag="ACHKXP_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7647477.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            21346..22206
                     /gene="sgbU"
                     /locus_tag="ACHKXP_002495"
     CDS             21346..22206
                     /gene="sgbU"
                     /locus_tag="ACHKXP_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO7647478.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22200..22895
                     /gene="araD"
                     /locus_tag="ACHKXP_002496"
     CDS             22200..22895
                     /gene="araD"
                     /locus_tag="ACHKXP_002496"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7647479.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKAVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTTEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(22927..23028)
                     /locus_tag="ACHKXP_002497"
                     /pseudo
     CDS             complement(22927..23028)
                     /locus_tag="ACHKXP_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            23032..23295
                     /locus_tag="ACHKXP_002498"
                     /pseudo
     CDS             23032..23295
                     /locus_tag="ACHKXP_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23242..23982)
                     /gene="yiaT"
                     /locus_tag="ACHKXP_002499"
     CDS             complement(23242..23982)
                     /gene="yiaT"
                     /locus_tag="ACHKXP_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO7647480.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            24106..25080
                     /gene="yiaU"
                     /locus_tag="ACHKXP_002500"
     CDS             24106..25080
                     /gene="yiaU"
                     /locus_tag="ACHKXP_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7647481.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(25077..26213)
                     /gene="yiaV"
                     /locus_tag="ACHKXP_002501"
     CDS             complement(25077..26213)
                     /gene="yiaV"
                     /locus_tag="ACHKXP_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7647482.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(26219..26542)
                     /gene="yiaW"
                     /locus_tag="ACHKXP_002502"
     CDS             complement(26219..26542)
                     /gene="yiaW"
                     /locus_tag="ACHKXP_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7647483.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            26665..26868
                     /locus_tag="ACHKXP_002503"
     CDS             26665..26868
                     /locus_tag="ACHKXP_002503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647484.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            complement(27087..28625)
                     /gene="aldB"
                     /locus_tag="ACHKXP_002504"
     CDS             complement(27087..28625)
                     /gene="aldB"
                     /locus_tag="ACHKXP_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO7647485.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(28790..29941)
                     /gene="yiaY"
                     /locus_tag="ACHKXP_002505"
     CDS             complement(28790..29941)
                     /gene="yiaY"
                     /locus_tag="ACHKXP_002505"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO7647486.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGNIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(30131..31975)
                     /gene="selB"
                     /locus_tag="ACHKXP_002506"
     CDS             complement(30131..31975)
                     /gene="selB"
                     /locus_tag="ACHKXP_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO7647487.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDSVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPIRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGLGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(31972..33363)
                     /gene="selA"
                     /locus_tag="ACHKXP_002507"
     CDS             complement(31972..33363)
                     /gene="selA"
                     /locus_tag="ACHKXP_002507"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO7647488.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSKKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(33461..34069)
                     /gene="yibF"
                     /locus_tag="ACHKXP_002508"
     CDS             complement(33461..34069)
                     /gene="yibF"
                     /locus_tag="ACHKXP_002508"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO7647489.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            34298..>35288
                     /locus_tag="ACHKXP_002509"
     CDS             34298..>35288
                     /locus_tag="ACHKXP_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7647490.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVLRLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPSYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAVVYDRSG"
BASE COUNT         8600 a         9289 c         8910 g         8489 t
ORIGIN      
        1 tcccccaccc ggtcaggcag gggataacgt ttacgccatt aatggcagaa gttgctgata
       61 gaggcgacgg aacgtttctc gtcgtggctg ataagcggca taacgctgcg catctggtag
      121 atgcgactgt tctaacggta gttgcggcaa caattcaatg agcgatttct ctggattcgc
      181 cgcgatctgc gccagccttg ctgcgcccag tgctggcccc acatcccccc ccgtacggta
      241 atcgagctgc tgaccgctga tatccgccaa catctgacgc cagtactcac tacgcgcccc
      301 gccaccaatc aacgtaacac tttgcggttt aatgccgcag gcatgcacga catccatgcc
      361 atctgccagc gcataaccca cgccttccag cactgctcgc gccagttcat tggggccatg
      421 ttgatgagtc aaaccaaaga aaaccccctt cgcctgggga ttattgtgtg gcgtacgctc
      481 gccggaaaga taaggcagaa accaaactgg ctcggcactt tcatcagcct gttgagctgc
      541 agcgattaaa gctggaacat tgctcaggcc ggttaatttc gcggcccaat ccagacacga
      601 cgccgcactc agcattacag acattaaatg ccaacgttgc ggtagcgcat ggcaaaagct
      661 gtgtacggcg ctttctggct tgcttaagaa cccttcgctg acagcaaaat agacccccga
      721 cgtccccagc gataacattg cctgattagc atcaaccatt cccacaccaa ccgcaccagc
      781 tgcattgtcg ccaccgcctg cgacaactgg caccgtcgcc ataccccacg ctttcgcaac
      841 ttcaggtaac aaagcaccag taatttcgct gccttcgtat aatgcgggca tctggtcacg
      901 agataagtcg caagcctgca gcatgacgtc actccagtca cgctttgcga catccagcca
      961 catggtgcca gctgcgtcag acatatcgct ggcaaactcc cccgtcatac gcagacgcaa
     1021 gtaatctttc ggtaataata ctttgtcgat ttgacggaat atctccggct catgccgctg
     1081 aacccatagc aatttaggcg cagtaaatcc gggcatcatc aggttgccgg taatcacccg
     1141 cgattgcgga actcgcgctt ccagcaaagt gcactcttgc gcacagcgcc cgtcgttcca
     1201 caaaatggca gggcgtaaca cccgttgctg agcatccagc aaggttgctc cgtgcatctg
     1261 gccggcaata cccaatgctt taacgtcctg cagagaatgc tgatcgccca gagctttcat
     1321 tgcgcgatca gttgcctgcc accactgttc cgggtcttgt tccgaccaga gtggatgcgg
     1381 gcgcgaaacg gtcagctttt ccgtttgcgc agcaaccacc tcaccctgct cgttgagcaa
     1441 aataactttt acgcccgagg tgccaagatc tatcccgata tacatatcga tcgttcctta
     1501 aaaaaatgcc cggtatcgct accgataacc gggccaacgg actgcacagt tagccgttat
     1561 ttgtcgaaca gataatggtt taccagattt tccagttgtt cctggcgacc actctgatgc
     1621 accggagaca aatgatgttc ctgagcatat ttggctaaat ctgccagtga catttggcct
     1681 ttcaggattt gctggcccaa ttcgctattc cagccggaat aacgctgcgc gatgcgttta
     1741 tccagctcgc catcttcaat catgcgcgct gcaattttca gcgccagtgc catcgtatcc
     1801 atcgcgccga tatgaccgta aaacagatca tatttatcag tactttgacg acgtactttg
     1861 gcatcgaagt tcagaccacc ggtggtgaaa ccgcctgctt tgagaatttc atacatcacc
     1921 agcgcattct cttccacact gttcgggaac tggtcggtgt cccagcccag ttgcgcatcg
     1981 ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa tggcggtggc tatttcatga
     2041 tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa tgttcagttt aatctctttt
     2101 tccagaccaa actgtttcag gaagccatag accgtcgcgg catcgtaatc atattgatgt
     2161 ttggtcggtt cttgcggttt cggttcgata agcaacgtgc cctggaaacc gattttatgt
     2221 ttatgctcaa ccaccatctg cataaagcgg cccagttgtt cacgctcctg acgcaagtcg
     2281 gtatttaaca gcgtttcgta accttcacga ccgccccaca ggacatagtt ttcaccgccc
     2341 aatttatggg ttgcttccat cgctgtaaca acttgcgttg ccgcccagct gaagacttca
     2401 ggatctgggt tcgtcgccgc acccgcgccg tagcgagggt ttgtaaagca gttggcggtt
     2461 ccccacagca gcttcacgcc gctctcttct tgcttgcctg ccaggacatc aaccatttgc
     2521 gcaaaattat tgatgtactc ttttaacgac gcgccctcag gggaaacatc cacatcgtgg
     2581 aagcaataaa atggcacatg taacttgtgg aaaaactcaa atgcgacatc tgctttacgc
     2641 ttcgccaacg ccagtgcctc accaggctgc tgccacggac gattaaacgc ccccacacca
     2701 aacatatccg ccccgttcca gcagaaggtg tgccagtagc aggcggcaaa acgcaagtgc
     2761 tcttccatac gcttacccaa caccagttcg tcgggattgt agtgacggaa tgctaacggg
     2821 tttgaggatt ttgagccttc ataacgaacg cgatcgagct ggtcaaaata ggcttgcata
     2881 ttgaactcca taatcaggta atgccgcggg tgatggatga tgtcgtaata ttgggcactc
     2941 cctttcagtt gctcaattat gttatttcac actgctattg agataattca caagtgtgcg
     3001 ctcgctcgca aaataaaatg gaatgatgaa actgggtaat tcctcgaaga gaaaaatgca
     3061 ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata aatcaagaaa taaaccaaaa
     3121 atcgtaatcg aaagataaaa atctgtaatt gttttcccct gtttagttgc taaaaattgg
     3181 ttacgtttat cgcggtgatt gttacttatt aaaactgtcc tctaactaca gaaggcccta
     3241 caccatgaaa ataaagaaca ttctactcac cctttgcacc tcactcctgc ttaccaacgt
     3301 tgctgcacac gccaaagaag tcaaaatagg tatggcgatt gatgatctcc gtcttgaacg
     3361 ctggcaaaaa gatcgagata tctttgtgaa aaaggcagaa tctctcggcg cgaaagtatt
     3421 tgtacagtct gcaaatggca atgaagaaac acaaatgtcg cagattgaaa acatgataaa
     3481 ccggggtgtc gatgttcttg tcattattcc gtataacggt caggtattaa gtaacgttgt
     3541 aaaagaagcc aaacaagaag gcattaaagt attagcttac gaccgtatga ttaacgatgc
     3601 ggatatcgat ttttatattt ctttcgataa cgaaaaagtc ggtgaactgc aggcaaaagc
     3661 cctggtcgat attgttccgc aaggtaatta cttcctgatg ggcggctcgc cggtagataa
     3721 caacgccaag ctgttccgcg ccggacaaat gaaagtgtta aaaccttacg ttgattccgg
     3781 aaaaattaaa gtcgttggtg accaatgggt tgatggctgg ttaccggaaa acgcattgaa
     3841 aattatggaa aacgcgctaa ccgccaataa taacaaaatt gatgctgtag ttgcctcaaa
     3901 cgatgccacc gcaggtgggg caattcaggc attaagcgcg caaggtttat cagggaaagt
     3961 agcaatctcc ggccaggatg cggatctcgc aggtattaaa cgtattgctg ccggtacgca
     4021 aactatgacg gtgtataaac ctattacgtt gttggcaaat actgccgcag aaattgccgt
     4081 tgagttgggc aatggtcagg aaccaaaagc agataccaca ctgaataatg gcctgaaaga
     4141 tgtcccctcc cgcctcctga caccgatcga tgtgaataaa aacaacatca aagatacggt
     4201 aattaaagac ggattccaca aagagagcga gctgtaagcg ttacgcccca gcgcggagcg
     4261 ggggcgtgat ttctctccat gccgcgtgaa tgaattggct taggtggagt cgttatgcct
     4321 tatctacttg aaatgaagaa cattaccaaa accttcggca gtgtgaaggc gattgataac
     4381 gtctgcttgc ggttgaatgc tggcgaaatc gtctcacttt gtggggaaaa tgggtctggt
     4441 aaatcaacgc tgatgaaagt gccgtgtggt atttatcccc atggctccta cgaaggcgaa
     4501 attatttttg cgggagaaga gattcaagcg agtcacatcc gcgataccga acgcaaaggt
     4561 atcgccatca ttcatcagga attggccctg gtgaaagaat tgaccgtgct ggaaaatatc
     4621 ttcctgggta acgaaataac ccacaatggc attatggatt atgacctgat gacgctacgc
     4681 tgtcagaagc tgctcgcaca ggtcagttta tccatttcac ctgatacccg cgttggcgat
     4741 ttagggcttg ggcaacaaca actggttgaa attgccaagg cacttaataa acaggtgcgc
     4801 ttgttaattc tcgatgaacc gacagcctca ttaactgagc aggaaacgtc ggttttactg
     4861 gatattattc gcgatctaca acagcacggt atcgcctgta tttatatttc gcacaaactc
     4921 aacgaagtca aagcgatttc cgatacgatt tgcgttattc gcgacggaca gcacattggt
     4981 acgcgtgatg ctgccggaat gagtgaagac gatattatca ccatgatggt cgggcgagag
     5041 ttaaccgcgc tttaccctaa tgaaccacat accaccggag atgaaatatt acgtattgaa
     5101 catctgacgg catggcatcc ggttaatcgt catattaaac gagttaatga tgtctcgttt
     5161 tccctgaaac gtggcgaaat attgggtatt gccggactcg ttggtgccgg acgtaccgag
     5221 accattcagt gcctgtttgg tgtgtggccc ggacaatggg aaggaaaaat ttatattgat
     5281 ggcaaacagg tagatattcg taactgtcag caagccatcg cccaggggat tgcgatggtc
     5341 cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg cggttggtaa aaatattacc
     5401 ctcgccgcac tcaataaatt taccggtggc attagccagc ttgatgacgc ggcagagcaa
     5461 aaatgtattc tggaatcaat ccagcaactc aaagttaaaa cgtcgtcccc cgaccttgct
     5521 attggacgtt tgagcggcgg caatcagcaa aaagcgatcc tcgctcgctg tctgttactt
     5581 aacccgcgca ttctcattct tgatgaaccc accaggggta tcgatattgg cgcgaaatac
     5641 gagatctaca aattaattaa ccaactcgtc cagcagggta ttgccgttat tgtcatctct
     5701 tccgaattac ctgaagtgct cggccttagc gatcgtgtac tggtgatgca tgaagggaaa
     5761 ctaaaagcca acctgataaa tcataacctg actcaggagc aggtgatgga agccgcattg
     5821 aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg aaattggccg taccgacatc
     5881 cggtggcttc tccgggctga aatcactgaa tttgcaggtc ttcgtgatga ttgcagctat
     5941 catcgcaatc atgctgttct ttacctggac caccgatggt gcctacttaa gcgcccgtaa
     6001 cgtctccaac ctgttacgcc agaccgcgat taccggcatc ctcgcggtag gaatggtgtt
     6061 cgtcataatt tctgctgaaa tcgacctttc cgtcggctca atgatggggc tgttaggtgg
     6121 cgtcgcggcg atttgtgacg tctggttagg ctggcctttg ccacttacca tcattgtgac
     6181 gctggttctg ggactgcttc tcggtgcctg gaacggatgg tgggtcgcgt accgtaaagt
     6241 cccttcattt attgtcaccc tcgcgggcat gttggcattt cgcggcatac tcattggcat
     6301 caccaacggc acgactgtat cccccaccag cgccgcgatg tcacaaattg ggcaaagcta
     6361 tctccccgcc agtaccggct tcatcattgg cgcgcttggc ttaatggctt ttgttggttg
     6421 gcaatggcgc ggaagaatgc gccgtcaggc tttgggttta cagtctccgg cctctaccgc
     6481 agtagtcggt cgccaggctt taaccgctat catcgtatta ggcgcaatct ggctgttgaa
     6541 tgattaccgt ggcgttccca ctcctgttct gctgctgacg ttgctgttac tcggcggaat
     6601 gtttatggca acgcggacgg catttggacg acgcatttat gccatcggcg gcaatctgga
     6661 agcagcacgt ctctccggga ttaacgttga acgcaccaaa cttgccgtgt tcgcgattaa
     6721 cggattaatg gtagccatcg ccggattaat ccttagttct cgacttggcg ctggttcacc
     6781 ttctgcggga aatatcgccg aactggacgc aattgcagca tgcgtgattg gcggcaccag
     6841 cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg ggggcattta tcatggcttc
     6901 actggataac ggcatgagta tgatggatgt accgaccttc tggcagtata tcgttaaagg
     6961 tgcgattctg ttgctggcag tatggatgga ctccgcaacc aaacgccgtt cttgattttg
     7021 ataaaaattt tctcaaagcc ggttacgtat taccggtttt gagtttttgc atgattcagc
     7081 aggaaaagaa ccatgtttac taaacgtcac cgcatcacat tactgttcaa tgccaataaa
     7141 gcctatgacc ggcaggtagt agaaggcgta ggggaatatt tacaggcgtc acaatcggaa
     7201 tgggatattt tcattgaaga agatttccgc gcccgcattg ataaaatcaa ggactggtta
     7261 ggagatggcg tcattgccga cttcgacgac aaacagatcg agcaagcgct ggctgatgtc
     7321 gacgtcccca ttgttggggt tggcggctcg tatcaccttg cagaaagtta cccacccgtt
     7381 cattacattg ccaccgataa ctatgcgctg gttgaaagcg catttttgca tttaaaagag
     7441 aaaggcgtta accgctttgc tttttatggt cttccggaat caagcggcaa acgttgggcc
     7501 actgagcgcg aatatgcatt tcgtcagctt gtcgccgaag aaaagtatcg cggagtggtt
     7561 tatcaggggt tagaaaccgc gccagagaac tggcaacacg cgcaaaatcg gctggcagac
     7621 tggctacaaa cgctaccacc gcaaaccggg attattgccg ttactgacgc ccgagcgcgg
     7681 catattctgc aagtatgtga acatctacat attcccgtac cggaaaaatt atgcgtgatt
     7741 ggcatcgata acgaagaact gacccgctat ctgtcgcgtg tcgccctttc ttcggtcgct
     7801 cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt tgcatcgatt attagataaa
     7861 gaagaaatgc cgctacagcg aattttggtc ccaccagttc gcgtcattga acggcgctca
     7921 acagattatc gctcgctgac cgatcccgcc gttattcagg ccatgcatta cattcgtaat
     7981 cacgcctgta aagggattaa agtggatcag gtactggatg cggtcgggat ctcgcgctcc
     8041 aatcttgaga agcgttttaa agaagaggtg ggtgaaacca tccatgccat gattcatgcc
     8101 gagaagctgg agaaagcgcg cagtctgctg atttcaacca ccttgtcgat caatgagata
     8161 tcgcaaatgt gcggttatcc atcgctgcaa tatttctact ctgtttttaa aaaagcatat
     8221 gacacgacgc caaaagagta tcgcgatgta aatagcgagg tcatgttgta gcgatgatga
     8281 gaattgtcgg cgtcacatca ggtaacgctg cgtggttgtc ggatgcggcg tgaacgcctt
     8341 atccgaccta cccgccaggc atgataaaac gcaccaacaa cgcttcaggc tcgtagctca
     8401 actgcctgag acaaagtaaa aagccttatc cgactgacaa gtcggataag gctcaaggaa
     8461 atgcaattac atatgcgccg cgattaaccg ttggttatcc tggtacattg cgaacaggta
     8521 gttgttataa ctcttccctt tggtcgagta gcccttcagc ttgtgaatca tcgctgtggc
     8581 agtcacttcc tgatccgctt tacgcagctg cgcacgcgat ttacggaacg aagagtaagc
     8641 cgggtgcgta ttcaggttag tgacataggc gctcaccgat tctttgacag aactaaactg
     8701 tgagtaccct ttcactttac ctggcgcatt ggtacaacgt cctttcatgc atttcatgcc
     8761 gaacaggttg ttgttgttgc gcgccagctt cgacgttccc caaccgcttt ctgctgcagc
     8821 catcgtcgcc accatactgg tggggataat gtctacgcgt tcaagcaagg tattccacgg
     8881 gatttttcgc gtattaccgg accacttcac cttgtagcgt ttggcgatgt ctttcagacg
     8941 cgcacgctca gcaggtgacc attggccctg atactgtttt gaaatgagcc agttacgttc
     9001 cgcagtaatg gccgcatttt ggctggtaat gtaaggcatt acggtccgga gaaacgcctt
     9061 tttccttggt gttccggaag ggtattttcg caaatcagga agtgaactac tctttgcact
     9121 attgcgagaa tactcttgtt tactgcttac ctgtttatta ctagctttag ttaagtggga
     9181 cttttgactc gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa acaccagagt
     9241 gagtaacata agaatcatcg ccccatatcg tcgtatggga gtcaaaatca tcaggtctcc
     9301 tggtcggatt taatcattcc aacaccttat atttttcaca aatttgagag ttgaatctca
     9361 aatcatatca aaaatagctg tcaagagcac cccaaggaat agtccaaatc tgaaactatg
     9421 tcacgtgtta acgattcaga ttggcgctaa atcgcagaaa atgtgggggt tatcgcaaaa
     9481 ttcagccgtt ttttgcgcga gatcgctcac ccttgcttct catcctgtgg acttaccgct
     9541 cagggatgag ttttgtttgg cttatcgctg gcaaactgtc tgaaatcgca gcaataagga
     9601 ctcatccgcc atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc
     9661 cgccagctgg acttctccgg ggtttcccgc ctttagcgaa caggggacag gaacatttgt
     9721 cagccacgcg cagttgccca aaggtacgcg tccactaacg ctaaattttg accaacagtg
     9781 ctggcagcct gcggatgcga taaaactcaa tcagatgctt tccctgcaac cttgtagcaa
     9841 cacgccgcct caatggcgat tgttcaggga cggcgaatat acgctgcaaa tagacacccg
     9901 ctccggtacg ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaagcct
     9961 ggtccgtgaa tgcccgaaat gggatggatt accgctcaca gtggatgtca gcgccacttt
    10021 cccggaagga gccgccgtac gggattatta cagccagcaa attgcgatag tgaagaacgg
    10081 tcaaataatg ttacaacccg ctgccaccag caacggttta ctcctgctgg aacgggcaga
    10141 aactgacaca tccgcccctt tcgactggca taacgccacg gtttactttg tgctgacaga
    10201 tcgtttcgaa aacggcgatc ccagtaatga ccagagttac ggacgtcata aagacggtat
    10261 ggcggaaatt ggcacttttc acggcggcga tttacgcggc ctgaccaaca aactggatta
    10321 cctccagcag ttgggcgtta atgctttatg gataagcgcc ccatttgagc aaattcacgg
    10381 ctgggtcggc ggcggtacaa aaggcgattt cccgcattat gcctaccacg gttattacac
    10441 acaggactgg acgaatcttg atgccaatat gggcaacgaa gccgatctac ggacgctggt
    10501 tgatagcgca catcagcgcg gtattcgtat tctctttgat gtcgtgatga accacaccgg
    10561 ctatgccacg ctggcggata tgcaggagta tcagtttggc gcgttatatc tttctggtga
    10621 cgaagtgaaa aaatcgctgg gtgaacgctg gagcgactgg aaacctgccg ccgggcaaac
    10681 ctggcatagc tttaacgatt acattaattt cagcgacaaa acaggctggg ataaatggtg
    10741 gggaaaaaac tggatcagaa cggatatcgg cgattacgac aatcctggat tcgacgatct
    10801 cactatgtcg ctagcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct
    10861 gccggtgttc tataaaaaca aaatggatac ccacgccaaa gccattgacg gctatacgcc
    10921 gcgcgattac ttaacccact ggttaagtca gtgggtccgc gactatggga ttgatggttt
    10981 tcgggtcgat accgccaaac atgttgagtt gcccgcctgg cagcaactga aaaccgaagc
    11041 cagcgccgcg cttcgcgaat ggaaaaaagc taaccccgac aaagcattag atgacaaacc
    11101 tttctggatg accggtgaag cctggggcca cggcgtgatg caaagtgact actatcgcca
    11161 cggcttcgat gcgatgatca atttcgatta tcaggagcag gcggcgaaag cagtcgactg
    11221 tctggcgcag atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt
    11281 gttgagctac ctctcgtcgc atgatacccg cctgttccgt gaagggggcg acaaagcagc
    11341 agagttatta ctattagcgc caggcgcggt acaaatcttt tatggtgatg aatcctcgcg
    11401 tccgttcggt cctacaggtt ctgatccgct gcaaggtaca cgttcggata tgaactggca
    11461 ggatgttagc ggtaaatctg ccgccagcgt cgcgcactgg cagaaaatca gccagttccg
    11521 cgcccgccat cccgcaattg gcgcgggcaa acaaacgaca cttttgctga agcagggcta
    11581 cggctttgtt cgtgagcatg gcgacgataa agtgctggtc gtctgggcag ggcaacagta
    11641 acttttccgg cttcccgttc gtcagtacct cgggaagccg ccaaccagga taaaatgtca
    11701 gccctaatca gcgttgcagg ataaagcacc gctcactctt caacagaccg atttgcaccc
    11761 cagcaaatgt agcgttattg ttaccttcct tgctacagag ttcgacagat atcccgctat
    11821 gacattctcc ctttttggtg acaaatttac ccgccactcc ggcattacgc tgttgatgga
    11881 agatctgaac gacggtttac gcacgcctgg cgcgattatg ctcggcggcg gtaatccggc
    11941 gcagatcccg gaaatgcagg actacttcca gacgctactg accgacatgc tggaaagtgg
    12001 caaagcgact gatgcactgt gtaactacga cggtccacag gggaaaacgg agctactcac
    12061 actgcttgcc ggaatgctgc gcgagaagtt gggttgggat atcgaaccac agaatattgc
    12121 actaacaaac ggcagccaga gcgcgttttt ctacttattt aacctgtttg ccggacgccg
    12181 tgccgatggt cgggtcaaaa aagtgctgtt cccgcttgca ccggaataca ttggctatgc
    12241 tgacgccgga ctggaagaag atctgtttgt ctctgcgcgt ccgaatattg aactgctgcc
    12301 ggaaggccag tttaaatacc acgtcgattt tgagcatctg catattggcg aagaaaccgg
    12361 gatgatttgc gtctcccggc cgacgaatcc aacaggcaat gtgattactg acgaagagtt
    12421 gctgaagctt gacgcgctgg cgaatcaaca cggcattccg ctggtgattg ataacgctta
    12481 tggcgtcccg ttcccgggta tcatcttcag tgaagcgcgc ccgctatgga atccgaatat
    12541 cgtgctgtgc atgagtcttt ccaagctggg tctacctggc tcccgctgcg gcattatcat
    12601 cgccaatgaa aaaatcatca ccgccatcac caatatgaac ggcattatca gcctggcacc
    12661 tggcggtatt ggtccggcga tgatgtgtga aatgattaag cgtaacgatc tgctgcgcct
    12721 gtctgaaaca gtcatcaaac cgttttacta ccagcgtgtt caggaaacta tcgccatcat
    12781 tcgccgctat ttaccggaaa atcgctgcct gattcataaa ccggaaggag ccattttcct
    12841 ctggctatgg tttaaggatt tgcccattac gaccgagcag ctctatcagc gcctgaaagc
    12901 acgcggcgtg ctgatggtgc cggggcacaa cttcttccca gggctggata aaccgtggcc
    12961 gcatacgcat caatgtatgc gcatgaacta cgtaccagag ccggagaaaa ttgaggcggg
    13021 ggtgaagatt ctggcggaag agatagaaag agcctgggct gaaagtcact aaaaattgcc
    13081 tgatgcgcta cgcttatcag gcctacatga tttctgcaat atattgaatt tggaagaatt
    13141 tgtaggccgg atagggcgtt tacgccgcat ctggcataaa caaagcacgc tattccggcg
    13201 ttgagaaacg ccggaaaacg tttcaaacag gctgctgccg tagccgatgc cgctgcactt
    13261 tctcgacatc catgcactgc aacgcctttg tcgggcaggc ttcaacacag gccggtcccg
    13321 tctcccgatg ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga
    13381 cctccatcgc accaaacggg caagccagca tacagctttt acagccaatg caacgtgttt
    13441 gttcaacgaa aatatgccct tgttcgcggc ttatcgcgtc aacagggcag acattcgcgc
    13501 acggtgcatc ttcacactga tgacaggcta ctgccgtggt ccagctgtgg tctttaatga
    13561 cacgaatacg ggaaataaac tcgtctggtg acaacgcagc gcaatcctga ttctcctgat
    13621 gcgacactgc gcaagccact tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa
    13681 taataaaccg gttcatgatc actcctttgc actaaaacag cagagtgcca gagagcgcgt
    13741 ccctactgct ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc caaagcgtta
    13801 tgtgatcgcg cccaggtcat ccctgacagt aaatcccagt tcattagaaa tagcctgcgc
    13861 ggtttcacgc agtggtttca ggagattttt ctctcccacc tgtttcagac gtgatgtcga
    13921 aagcgaaatc gacacggcgt acggcacccg cccatgaata tcaaacaccg gaacagcaat
    13981 acaggagacg ccgagttcgt tttcttctct gtccatcgcc gctccgcttt cacgaatgtg
    14041 cgccagttcg tcgaacatcg cgggcagctc ggtaatggta ttgcgggtta acggctggat
    14101 ctcatgctga tggctttccc agtatgactt cacgtagtcc gggtgaccaa acgccatgta
    14161 gatcttgccc attgcggaac agtagagcgg catatgctgg ccaatatagg cacgggttcg
    14221 cagcatcccg gttgtcggct ccagcttata aatcaaaata gcgtgatcgt cttcgcggct
    14281 ggagaagtta atggtttcac cagtggcgat gttcagtgcc tcaagatgcg gagcggcgat
    14341 atgaatgata ttcagcgaag acagcgcctt ctgcccgacg gcaataaatt tggtagtcag
    14401 gcggtaactc cctgcggcgg gcgcggtggt cacatagccg caggactgta atccctgcaa
    14461 taagcgatgg acggtactct tatttaaacc agccagctcc gaaagatgcg ccaacggaca
    14521 accgtttgga tagttgctca aaatctcaat cagcatcaac ccgcgaaaca gactctggct
    14581 tccggctgga cgctcttttt cctgcgccat ctcgttctct ttttttccca tcacttcttt
    14641 ccccattttg tcgcgtcctg atggtagcgc aaagtgtgcc gtagttcacg atctcgacag
    14701 ataatttata accacttgat ttttatgtat tttgaaattc atcaatcaga ttgctttgtt
    14761 aaaaagtgat cgatatattt gaaatcatgt ttcgcatatt gaaattttaa gccaaaaaag
    14821 cgatcaaaaa aacaaggaag cctgggatga aagttacatt tgagcagtta aaagcagcct
    14881 ttaatcgggt cttaatttca cgcggcgttg acagcgaaac ggctgacgcc tgtgcagaga
    14941 tgttcgcccg caccaccgaa tccggcgttt attctcacgg cgttaatcgt ttccctcgtt
    15001 tcattcaaca actggaaaac ggcgatatca ttcctgatgc ccaacccaaa cgtataacca
    15061 gcctcggcgc aattgaacag tgggacgccc agcgttcgat cggtaacctg acagcgaaaa
    15121 agatgatgga tcgcgccatt gaactggctg ccgatcacgg tattggtctg gtggcactac
    15181 gtaatgccaa ccactggatg cgcggcggca gctacggctg gcaggcggcg gaaaaaggct
    15241 atattggcat ttgctggacc aactccatcg ccgtaatgcc gccgtggggc gcaaaagagt
    15301 gtcgcatcgg cactaacccg ctgatcgtcg ccattccttc cacgccgatc accatggtcg
    15361 atatgtcgat gtcgatgttc tcttacggca tgttagaagt taaccgtctg gcaggtcgtc
    15421 agctcccggt agatggtggc tttgatgatg agggcaattt gaccaaagaa cctggcgtta
    15481 tcgagaagaa tcgccgcatt ttgccgatgg gctactggaa aggttctggc atgtcgattg
    15541 tgctggatat gatcgctact ctcctttccg acggcgcatc cgttgccgaa gtcacccagg
    15601 acaacagcga cgaatacggc atttcacaaa tttttattgc cattgaagtg gacaagctta
    15661 tcgacggtcc cacccgcgat gccaagctgc aacgcatcat ggattacgtt actagtgccg
    15721 agcgtgctga cgaaaatcag gccattcgct tacccggcca tgaatttact accctgctgg
    15781 ccgaaaaccg ccgtaacggc atcactgttg atgacagcgt gtgggccaaa atccaggcgt
    15841 tatgaggaga taagtcatga tttttggaca tatcgcgcag cccaatccgt gccgtttgcc
    15901 cgccgccatt gaaaaggcgc tcgattttct gcgcgctacc gatttcaacg ccctggagcc
    15961 gggcgttgtc gaaatcgacg gcaaaaatat ttatacgcaa attatcgatt taaccacacg
    16021 cgaagcggtg gtaaaccgtc cggaagtgca tcgtcggtat atcgatattc agtttctggc
    16081 gtggggcgaa gagaaaatcg gcattgctat tgatacggga aataataaag tcagcgaatc
    16141 actattagag cagcgcgata ttatttttta tcacgacagt gaacatgaat catttataga
    16201 aatgataccg ggcagctacg caattttctt tccgcaggat gttcaccgac caggttgtat
    16261 tttgcaaaca gcctctgaga tacgaaaaat tgtggttaaa gtcgcattaa cggcgctgaa
    16321 ttaatcgaat aatcgtctac gccagaacga ctgggtaatg ttattgctct aactatatta
    16381 ataatttagg ttgctgcaca tgcaacttga attatgacag acatagcctc aaggaatagc
    16441 tatgaaaaaa atacttgaag caatactggc gattaatctc gccgtacttt cctgtattgt
    16501 gtttataaac attattttaa gatatggttt tcagacaagc attttatctg tagatgaatt
    16561 gtcacgttat ttatttgtct ggttaacatt tattggcgcg attgtggctt ttatggataa
    16621 cgcccacgtt caggtcacct ttctggtgga gaaactctcc cccgcatggc aacgacgagt
    16681 cgctctggtt acccattcct taatcttatt tatttgtggc gcactggcct ggggcgcaac
    16741 gctaaaaaca attcaggact ggagcgatta ttcaccgatc ctcggtttac ccatcggcct
    16801 gatgtatgcc gcttgtctgc ccaccagcct cgttatcgca ttctttgaac tgcgtcattt
    16861 atatcaactc atcacgcgta gcaattctct aacgtcgcca ccgcaaggag cttaatcatg
    16921 gctgtgctga tttttctggg ctgtctgttg ggtggtattg ctatcggttt gcctattgcc
    16981 tgggcactgt tgttgtgcgg ggcggcgtta atgttctggc tggacatgtt tgatgtccag
    17041 atcatggcac aaacgctggt gaacggagcc gatagcttct ccctgctggc gattccgttc
    17101 tttgtgctgg cgggtgaaat aatgaatgcg ggcggcttgt caaaacgcat tgttgacctg
    17161 ccgatgaaac tggtggggca taaaccaggc gggctgggct acgtcggcgt gctggcagct
    17221 atgattatgg cgagcctttc tggttctgcc gttgcggata ccgccgccgt tgccgctcta
    17281 ctggtgccga tgatgcgcag cgccaactac ccggtcaacc gggcggcggg gctgattgcc
    17341 tctggcggca ttatcgcgcc aattattccc ccttccattc cgtttattat cttcggcgtt
    17401 tccagcggat tatccatcag taagctgttt atggcgggca ttgcccctgg catgatgatg
    17461 ggcgcaacgc tgatgcttac ctggtggtgg caggccagtc gccttaattt accccgccag
    17521 caaaaagcaa cgatgcagga aatctggcac tcttttgtct ccggtatctg ggcgctgttt
    17581 cttccagtca tcattattgg cgggtttcgc tccggtctgt ttacccctac cgaagccggg
    17641 gccgttgctg ctttctatgc gctgtttgtc gccacagtta tttaccgtga aatgaccttc
    17701 gccacacttt ggcatgtact cattggcgcg gcgaaaacca cctcagtggt tatgtttctg
    17761 gtggcctcgg cacaagtttc cgcctggctg attaccattg ctgaactgcc gatgatggtt
    17821 tctgatttac tgcaaccgtt ggtcgattca ccgcgtctgc tgtttatcgt cattatggtg
    17881 gctatcctga ttgtcggcat ggtcatggat ttaacgccga cggtattaat tcttaccccg
    17941 gtattaatgc ctttagttaa agaggcagga atcgatccga tttatttcgg tgtcatgttt
    18001 atcattaact gctcaatcgg cttaattaca ccgcctatcg gtaatgtact taacgttatt
    18061 tcaggggtgg caaaactcaa attcgatgat gcagtcagag gcgttttccc ttacgtcctg
    18121 gttttatatt cattattagt cgtatttgtt tttattcccg atctcatcat cctccctttg
    18181 aaatggatta attaaaagga aaatattatg aaattacgct ctgtaaccta cgcattattc
    18241 attgctggcc tggctgcatt cagcacatct tctctggcgg cgcaatcttt acgtttcggt
    18301 tatgaaacct cacaaaccga ctcgcaacat attgcggcga aaaaattcaa tgatttattg
    18361 caggagagaa ccaaaggcga gctgaaatta aaattgtttc cggatagcac cctcggtaac
    18421 gcgcaggcga tgatcagcgg cgtacgtggc ggcaccatcg atatggaaat gtccggctcg
    18481 aataactttg ccgggttatc accagtgatg aacttgcttg atgtcccttt cctgttccgc
    18541 gataccgctc acgcgcataa aacgctcgac ggcaaagtcg gtgatgatct gaaagcctca
    18601 cttgaaggta aaggactaaa agtactggcc tactgggaaa acggctggcg cgatgtcacc
    18661 aactcgcgcg caccggttaa aacccccgcc gacctgaaag ggctgaaaat tcgcaccaac
    18721 aatagcccga tgaatatcgc cgcattcaaa gtctttggcg ctaacccgat cccgatgccg
    18781 tttgccgaag tctataccgg gctggaaacc cgcactatcg acgctcagga acacccgatc
    18841 aacgtcgtct ggtcagcaaa atttttcgaa gtgcagaagt acctttctct gacgcaccac
    18901 gcctattccc cgcttctggt ggtgatcaac aaagcgaagt ttgatggctt aaccccggag
    18961 ttccagcaag cgctaatttc atctgcgcag gaagcgggta actaccagcg caaactggtt
    19021 gccgaagatc agcaaaaaat catcgacggc atgaaagaag cgggcgtgga agtcatcacc
    19081 gatctcgacc gcaaagcctt tagcgacgca ctgggtactc aggtccgcga catgtttgta
    19141 aaagatgttc cgcagggtgc tgatctactg aaagccgtgg atgaggtgca ataaaccatg
    19201 acgcaatact ggctggggtt agattgtggc ggtagctggc tgaaagccgg gctgtatgac
    19261 cgcgaaggcc gggaggcagg cgtgcagcgc ctgccgctgt gcgcattaag cccgcagcca
    19321 ggttgggcag agcgcgatat ggcagaactg tggcaatgct gcatggctgt cattcgcgcc
    19381 ctgcttactc attctggtgt tagcggggaa caaattgtcg gtatcggcat ctccgcacag
    19441 ggaaagggct tgtttttgct ggataaaaac gacaaaccgc tcgggaatgc tattttgtcc
    19501 tcggaccgcc gggcgatgga aatcgttcgt cgctggcagg aagatggcat cccggaaaaa
    19561 ctctacccgc tgacccgaca aaccttgtgg accgggcatc cggtgtcgct gttacgctgg
    19621 ctgaaagagc acgaaccaga acgctacgcg caaattggct gcgtgatgat gacgcacgac
    19681 tacctgcgct ggtgtttaac tggcgtcaaa ggctgtgaag agagcaatat ttccgagtcc
    19741 aacctctaca acatgagtct tggggaatat gacccgtgcc tcaccgactg gctggggatc
    19801 gctgaaatca atcacgccct gccgcctgtt gtcggatctg ccgaaatctg cggggagatc
    19861 accgctcaga cagccgccct gaccggtctg aaagcgggta cgcccgttgt tggcggcctg
    19921 tttgatgtgg tttccaccgc actctgcgcc gggatcgaag acgaatttac cctcaatgcg
    19981 gtgatgggga cctgggcggt gaccagcggc ataacccgcg gtttacgtga cggtgaagcg
    20041 catccgtatg tctatggtcg ctacgttaac gatggtgaat ttatcgttca cgaagccagc
    20101 cctacctctt ccggcaacct cgaatggttt accgcacagt ggggagaaat ctcgtttgat
    20161 gagatcaatc aggccgttgc cagcttgccg aaggctgggg gcgatctctt tttcctgccg
    20221 ttcctgtacg gcagcaacgc cggactcgag atgaccagtg gtttctacgg gatgcaggcc
    20281 attcacaccc gcgcgcacct gttgcaggcc atctatgaag gggtggtgtt cagccatatg
    20341 acccacctca accgaatgcg cgaacgtttt actgatgttc acaccctacg cgtcactggc
    20401 ggcccggcgc actccgatgt ctggatgcaa atgctggcgg acgtcagcgg tctgcgtatc
    20461 gagctgccgc aggtggaaga aaccggctgc tttggtgcgg cccttgccgc ccgcgtcggc
    20521 accggggttt atcacaactt cagcgaagcc caacgtgact tgcgacaccc ggtgcgcacc
    20581 ctgctgccag atatgaccgc ccatcagctt taccaaaaaa aatatcaacg ttatcagcat
    20641 ctcattgccg cacttcaggg ctttcacgcc cgcattaagg agcacacatt atgagccgac
    20701 cacttctgca actggccctc gaccactcat cacttgaagc cgcgcagcgc gacgtgacgc
    20761 tgttaaaaga cagcgtcgat atcgtcgaag cgggcaccat tctctgttta aacgaagggc
    20821 ttggcgcggt gaaagccttg cgcgaacagt gcccggacaa aatcatcgtt gctgactgga
    20881 aggtcgccga cgctggtgaa acgctcgcgc aacaggcgtt tggcgcaggc gctaactgga
    20941 tgaccatcat ctgcgccgcg ccgctcgcga cggtagaaaa aggccacgca atggcacaac
    21001 gctgcggggg tgaaattcag atagagctgt tcggtaactg gacgctggac gacgcccgcg
    21061 actggcatcg tattggcgtg cggcaggcca tttatcatcg cggtcgtgat gcacaggcca
    21121 gcgggcaaca gtggggcgaa gccgatctgg cacgcatgaa ggcgctttca gatatcggcc
    21181 ttgagctttc cattactggc gggattaccc ctgctgacct gccgctgttt aaagatatcc
    21241 gcgtgaaagc gtttattgcc gggcgggcac tggcaggcgc ggcaaatccg gcgcaagtgg
    21301 ctggcgattt ccatgcgcaa atcgacgcta tctggggagg tgcgcgtgcg taatcatccg
    21361 ttagggattt atgaaaaagc gctggcgaaa gatctctcct ggccggagcg gctggtgctg
    21421 gcaaaaagct gcggttttga ttttgtcgaa atgtcggtgg atgaaaccga cgaacggctc
    21481 tcacgtcttg actggagcgc cgcacaaagg acttcgctgg ttgccgcgat gatcgaaaca
    21541 ggtgttggga tcccgtcgat gtgcctgtcc gcacatcgtc gctttccctt tggtagccgt
    21601 gacgaggcag tgcgcgaacg ggcgcgggaa atcatgagta aagcgattcg cctggcgcgc
    21661 gatctcggca ttcgcaccat ccagctggca ggttatgacg tctattacga agaccacgac
    21721 gaaggcaccc ggcaacgttt tgctgaaggg ctggcgtggg cagtcgaaca ggcggcggca
    21781 tcacaagtaa tgctggcggt ggagattatg gataccgcgt ttatgaactc catcagcaaa
    21841 tggaaaaaat gggacgagat gctcgcctcg ccgtggttca ccgtctaccc ggacgtcggc
    21901 aacctcagcg cctggggcaa tgatgttccc gccgaactga aactgggcat tgaccgtatc
    21961 gccgcgatcc acctgaaaga cacccagcca gttaccgagc aaagtcccgg acagttccgc
    22021 gacgtgccgt ttggcgaagg ctgcgtcgat ttcgttggca tcttcaaaac gctgcataaa
    22081 ctgaactatc gcggttcttt cctgattgag atgtggaccg aaaaagccaa agagccggta
    22141 ctggagatta ttcaggcgcg gcgttggatt gaagcgcgta tgcaggaggc tggatttata
    22201 tgttagagca actgaaagcc gacgtgctgg cggcaaatct ggcgcttccc gctcaccatc
    22261 tggtgacgtt cacctggggc aatgtcagcg cggtagacga cacgcggcaa tggatggtaa
    22321 tcaaaccttc cggcgtcgaa tacgacgtga tgaccgccga cgatatggtg gtggttgaga
    22381 tagccagcgg taaggcggtg gaaggcagca aaaagccctc ttccgatacg ccaacgcatc
    22441 tggcgctcta ccgtcgctat gcagagatcg gtggcattgt gcatacccac tcgcgccacg
    22501 cgaccatctg gtcacaggcc gggctggatc tccccgcctg gggcaccacc cacgccgatt
    22561 atttttacgg tgccatcccc tgcacgcgcc agatgaccac tgaggagatc aacggcgagt
    22621 atgaatacca gactggcgaa gtgatcatta aaaccttcga agaacgtggt ttgaacccgg
    22681 cacaaatccc ggcggtactg gtacattctc acggcccgtt cgcctgggga aaaaacgccg
    22741 ccgatgcggt gcataacgcc gtggtgctgg aagagtgcgc ctatatgggc ctgttctcgc
    22801 gccagcttgc gccgcagctc cctgccatgc aaaacgaact gctggataaa cactacctgc
    22861 gtaagcatgg agacaatgcc tattacgggc agtaatccct cacgccgggg cttcatcgcc
    22921 ccggcactac gaattgatat gttccttgct gtaacgccgt ttccacgctg ctggcgttaa
    22981 accggtgtgt ttgcgaaaaa tctgccggaa aaagccaacg tcattaaacc gtattgcatg
    23041 gagcaggccg acaacgtcga gtcactgcat aacctgttgc tgcccgccga tgcgccattt
    23101 acgacatttg aaggcaaggg attgttcagc cataagatcc tgattcagac gccaggttcc
    23161 cggtatgaac agagcaatcc agagcaacaa cctttgtggc attacgacgg agcaccagcc
    23221 gcaccaacat ccaccggtga attaaaaacg ccagctcacg ccggtgacaa aggtaaaact
    23281 ctcattacga tcaatcatcg ggctgtcggt aatctcttcc ggcagctcgc tgtatcctgc
    23341 actcgccatc aatacgacgt gctctcctat cgggtatttt gccgtcaggc tgacataggg
    23401 cacccaggca tcctgcgcgg aataacttgc cagaccgcta cgacgggact cactctctga
    23461 aatgccatag taatagtcac tgaaattctc gtcataatag agcacgccca gcgcaggtgt
    23521 cagcgacagg cgacctatct gcattttgtg gaaaaccgat agctccccca cccagccgtt
    23581 gctgttatcc agaacgtccg cagccgcaga ggctttcaca gttccccaac gctcgtggtg
    23641 ataccaggca acccccgcca tagccgtact atcacgctta tcaagctgtt gcatggcata
    23701 atcgtcatta tcggcagggt caaattccag cggcatccag gatgcggtca ggctaagttc
    23761 atttttttca ctttgcgaca gaataaaacc taacgtggtc tgacgaacat aaaaagaatc
    23821 accttcataa ctaatcaggg gaattgcctt tgtattttca ttataaccgc gataaggcga
    23881 ttcattataa gccgcaccgg caccaatgga taattcagaa gcagttgcgc ttgccataaa
    23941 aggcaacgca aataacgcca caatattgcg gttaattaac atgttcggta ttccatttta
    24001 aaataagata aaaaagagtc ggcatagtaa tgcttacaat ttctccggtg caagattcat
    24061 atttatatag taatatataa aattatatat aattgggctg ttgcgatgac aaaattacaa
    24121 cttaagtacc gggagttaaa aattatctcg gtaatcgctg ccagtgaaaa tatcagccat
    24181 gccgcgactg tacttggcat cgcacaggcc aacgtcagca aataccttgc tgattttgaa
    24241 tcaaaagtgg gtttaaaagt ctttgaccga accacccggc agctgatgct cacacctttt
    24301 ggtaccgcgc tgttaccgta catcaacgac atgctggaca gaaacgagca gcttaataat
    24361 tttattgcag attataagca tgagaaacgt ggtcgggtca ccatctacgc cccaaccggc
    24421 ataattacct atttatccaa acatgtaatc gataaaatta aagatatcgg tgatatcacc
    24481 ttatcgctaa aaacctgcaa tcttgagcgt aatgcctttt atgaaggcgt cgaatttcct
    24541 gatgattgtg atgttttaat cagctacgct ccaccaaaag atgaatcgct ggttgccagt
    24601 tttattaccc aatatgccgt aaccgcttat gccagccagc gctatcttga gaaacatcct
    24661 attagccgtc ctgacgaact cgaacatcac tcctgtattt tgatcgactc gatgatgatt
    24721 gatgacgcga atatctggcg cttcaacgtc gccgggagta aagaggtgcg ggattatcgc
    24781 gtgaagggaa attacgtttg cgataacacg caatcggcgc tggagctggc acgaaatcac
    24841 ctggggattg tgtttgcgcc agacaaaagt gtgcagagcg atctgcaaga cggcacgctg
    24901 gtgccctgct ttcagcaccc gtatgaatgg tggctggatc tggtggctat cttccgtaaa
    24961 cgggaatacc agccctggcg ggtccagtat gtgctggatg aaatgctgcg tgaaattcgc
    25021 caccagcttg ctcagtcgca gcaactgcgg cctgaacaag cggcggaaag cgaagattaa
    25081 tgatcgagat aaaggtaatg cacccaactg gtcatgcgta acagcacttt gcgcatcacc
    25141 gagacatggc tgaaatgatc agagtagacc gccacctggg cgtaaatacc gtctggtaaa
    25201 gcgctcaaat cagtgtgctc atccagttca atggtcgcga taacgccatc tgaacccggc
    25261 gctgtgttta acgtctgtaa ggtgccggtc gactgataag ctccgccggg aacagctgga
    25321 ctaatggctg ccagcttacc gctgaacact ttgcctggca gagcgttaaa taccacttcc
    25381 gcatcatcgc cgggagccag gcgcagcaag gagttctgac ggaactgcgc cacgatttgt
    25441 cgtttctgat cgggtataaa caccatcacc ggacgtagcg gcagcgacgc ggcataggta
    25501 cccggacgaa tcagcacctg ggtcacataa ccatcgctcg gcgcacgcac tatcgtctgc
    25561 tcaaggttat attttgcttc cgcgagctgc gctttcaggc tggcgatttg agaatgttca
    25621 cccaacacca ggctatccag ctggctctgg atctgttttt gttccgccgc cgatgacttt
    25681 acggaggctt cctgcgccag ataattttgc cgcgccacat caatatcgcg ttctgaaaac
    25741 gggtttactt tcgcctggct gccgcgtgcg taacgctgat actctttagc gaatttatcc
    25801 cgcgtggcct ttgcctgctg agtattcgcc gccatctcat ctaactctgc gcccaacgcc
    25861 cgctgtttat gttctgcggt aacgatatcc gccatcagcc gatccacccg cgcctgataa
    25921 cgcgtcgggt ccagtcgaaa tagcacctca ccttttttaa tcagcgtatt tttcttatcc
    25981 gtcacttcga tcaccacgcc tgtcacctgt gggacaacag gaatagaaat aaccgctttt
    26041 tgcgctttga aggtatacgg atggttatag ttcattaaca gaattaaacc actgacaata
    26101 aatatgccac ccagggccgc tgtgggaatg gtccatttat ttacaggaat tttgaagatc
    26161 ttaaacattg cccatgcaaa agccacatag gtcaaaataa tcagtaaatc cataagtatt
    26221 actcggcaga agaggatttg atgtcggcga gctgtttctc cagcccggca atacgctgtt
    26281 gtagttgcac aacggaggag tcatggtttt gcattcccca tccccgctcc gggcggtaaa
    26341 gcgtggccca aatccacaaa aatggccaga taacatgcag cgtaaaaaga ctcacccaac
    26401 cagcaacatg aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa
    26461 tatcatgtaa aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca
    26521 gtgcaaaata atccagaaac atatttccct cagagaatat aaaaaagaac aattaacgca
    26581 tattatgaaa tcccatgatg cagtgcaatt tccttattta tataacggca tattgttttt
    26641 tatatacttt ctgaagtgtg ctgcgtggct tttcaacagg tcgaggttta tcatattgaa
    26701 ataatggaac tattgaccaa gctaatgaat aactccagta aaacatccac agtacagata
    26761 aagcgtatta aaccttcaat tatctaccgt ttattgctga ttggcctcgg aacgccaatg
    26821 gtgatttacg gcctggtttg cccgctcacc atcgaaacgc gagagtaaac tcctggcaaa
    26881 acacgcccca gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa
    26941 aacgtaaatt gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc
    27001 ggcctacggt gaggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga
    27061 atacccaatg cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc
    27121 accagcaggc acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg
    27181 atacctgatt gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg atagcagttg
    27241 gtccacacgc gcccggcctg aatgccgcgt cccatcttat aggccagatt accgttacga
    27301 ctccagacgc ctgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc
    27361 atcgttttga aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc
    27421 atattgttct gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct
    27481 tccagcagct tgcgccgccc gccggtgagc acgtctgcgc cctctttttt accgatatca
    27541 atgtagttga ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg
    27601 ctgtcgagtg ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag
    27661 cgttcgtaga ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcaccctgg
    27721 ttaaaggcaa acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc
    27781 atcacatcag caaagaagat atttggcgat ttgccgccca gctccagcgt caccggaata
    27841 atgttttgcg tggcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc
    27901 actttggcga tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccgttg
    27961 accacgttca ccacgcccgg cggcagtaaa tcaccgacga tttccattag cagcagtaca
    28021 gaaagcgggg taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga
    28081 gccattttcc agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg
    28141 cctaacggtt catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct
    28201 tcctgcgccc gaatacacga ggcgaaatag cgaaaatggt caatcgccag cggcacgtcg
    28261 gcagcactgg tttcgcgaat gggtttgccg ttatcccagg tttcagccgt cgctaacagc
    28321 tcaaggtttt gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc
    28381 gaggtgtgcg cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct
    28441 cgtttgcccg aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg
    28501 taatactcgc cgtcggcagg ggctacccat tcgccgccaa taaagttgtc atagcgggct
    28561 tttaacttga gggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg
    28621 gtcatgatcg tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct
    28681 tcgccagtcg gtaacagctt tacgacagct atcacgaatt tacgggcaag ggttacagag
    28741 atagttgaaa aagcggctga caatttgcca gccgttgtgg aaatgatgat tacatcgctg
    28801 cgcgataaat cgccacaatt tcttcgtgag ttgcctggat cgggttagta aaaccacagg
    28861 catctttcag ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc
    28921 gtaggcctgc cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt
    28981 cagcaccttc cgcatcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac
    29041 gcagacgtgc ggcggcgact ttgctgttga atacctgaac atgcggcagc aaaacggcgt
    29101 tacatacacc gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat
    29161 aacccagaga agcattattg aacgccatac cagcaaggaa ctgggcataa gccattgctt
    29221 cacgcgcttt cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg
    29281 tcacggcttt cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatatgctt
    29341 cgatagcgtg cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa
    29401 tcatcagaga ggagtcattg acagaaagca gcggagtgac atgtttatca acaatcgcca
    29461 ttttgatatg acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac
    29521 ccgcagtggt gttgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt
    29581 cgtaatcgcg aatattgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt
    29641 gtggagaacc accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca
    29701 aacctgcggc gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa
    29761 aaatattgcg ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca
    29821 tactgtcagt gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat
    29881 cagtcaatga atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca
    29941 taacacactc ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa
    30001 acatgatcat tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta
    30061 aacaatgtgc tttttttaaa atcataagaa atttgtatta atttatttta aaaattaatt
    30121 aatcatttcc ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac
    30181 gacgacgcgt aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc
    30241 ctacgcctaa gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat
    30301 cgcggatcat attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga
    30361 tcgcggtaat tattccttgc tgcgccgcct ggcgcagcgt caggcgcatt gcctgctcgt
    30421 cggttcccgt ctcttttgcc aggtcacgca cccaccacgg ctcgtcacca aacagcggct
    30481 ctgctttttg ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tctggcagat
    30541 gcagccaacc gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca
    30601 gtaccagcgc ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaagac
    30661 caggttcatc gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgctgcc
    30721 agcgggcggc aactggcgca ttcaacaagc tataaccagc ctgaatataa ccaggctgtt
    30781 gcagcaattc gcgcatccct tcgccgttga gctggcgcgc ccaggcgaaa tccgcaaggt
    30841 taaccgcgcc gcgttccaga tgaacagata acgcatcggc atcgctctgc gcccgtgcaa
    30901 gagacgccag ccattgcaga tattccggct tacgtttacc gcgacgcggc gggttaagca
    30961 tcaccacacg cgcccccgcc agcgtgttgc gggccgagat atcgcgcagc actaagcgat
    31021 cgttatccgc cagccacagc ggcgtgtcga acaccagctc ggcaaggtta tcttccagca
    31081 gcgaaacgcg tcccgtaacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg
    31141 tcagcggcgt atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcgcat
    31201 cggcaagcag ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgttaa
    31261 gcgcgatacg ctgcccggca tgggccgttt ctgttggctg gttttgtgca tgcagcgcac
    31321 gtacacggat cggtttattt acaccagtta gccagagtga atcgcctacc ttcacttccc
    31381 cgcttaacgc cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcga
    31441 tcgcgaggcg gaaactatgt tggctggcgt gttcacgttc tggtaactga agcagatgct
    31501 cgcgcagggc atcaattcca cgaccttcgg ttgctgcggt gataaacagt tttgcctcag
    31561 caaaaccgta ttcccgcagc acctccttta cctgacgttc aacctcatca acacgcgctt
    31621 cgtccacgcg atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca
    31681 aaatcgccag atgctcacgg gtctgtgcca tcacgctgtc atcgcacgcc accaccaaca
    31741 gcgcgtgatc gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg
    31801 gtacgtcgat aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat
    31861 cgatggtcat gccgcgcttt ttttcttccg gcagacggtc ggcatttacg ccagtaatcg
    31921 cctgcaataa ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa
    31981 caacatctcc aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc
    32041 gtcataaata cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc
    32101 aaggtggcta ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc
    32161 aaccggcagc gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc
    32221 cgcgccgtaa tgtgcggcaa ggggagcctg taaacgttgt gcctggattt gaatgacctc
    32281 tgcgctgcgg gtaagcaggc gcagggtcgg taatttttta ctcagagctt cagggtgtaa
    32341 ataaagacgc aacgtggctt ccagcgccgc aagggtcatt ttatccgcgc gtaatgcacg
    32401 cttcagcggg tggctttgca ggcgggcgat catctctttt ttaccaacaa taattcctgc
    32461 ctgcggcccg cctaacaact tgtcgccgga gaaactcacc agactgacgc ccgccgcaat
    32521 caactcctgc ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc
    32581 actgcctaaa tcagtcacta cgggaacatc cagctctttg ccgagcgcca ccagttccgc
    32641 ttcatctatc gctttggtga atccctgaat gctgtagtta ctggtatgta ctttcatcaa
    32701 cagcgcggta ttttcattca ccgcctgacg ataatcatta gcgtgcgtgc ggttggtggt
    32761 ccccacttcg tgtagggtgc agcctgcctg acgcatgaca tcaggaatac gaaacgcgcc
    32821 gccaatctcc accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc
    32881 caacatcaat aacaccgccg ccgcattgtt attaacgata caggcatctt ccgcccccgt
    32941 aatgcggcac agcagctgcg ccagcgcacg atcgcgatgt ccgcgtccgg cgtcgtccag
    33001 atcgtactcg agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc
    33061 cgcctgtaaa gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg
    33121 acgcagcgcg ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc
    33181 acaccatgcg ggcagcgtct ggctatcacg aatcacttct cgcgcttcgt caagcatctg
    33241 acgcagcaat tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct
    33301 atcgcgcaat aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt
    33361 cataggaaac ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac
    33421 ggattgtaac gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg
    33481 tgcgagcaaa actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcgat
    33541 cgacacacca gcctggcgca acgcggcgga aattgagata tccgacggca caggcaatgg
    33601 cgatagtcgc cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca
    33661 gcacatccag actacggttg attttctccc gctggcgtaa caattcatct tcagactgct
    33721 gcgccgctgg acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg
    33781 ccagtgcctc aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg
    33841 gagcgacatt cattaattca atatattcag cgatgatcgg cgaatcaaac caacattcgc
    33901 cctcttcggt caccagcacc ggcacttttc ctaacgggtt aaattgcgcc acgccgttgt
    33961 ccgcgttata gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa
    34021 tagaaagttt gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt
    34081 tgtgcgagga aaaatatcag tatggcctgg tgatgggaaa aagggcagaa aatgttgatg
    34141 gtttgttact tccaaataaa tcacatattt atcatggtga tataaatatt ttcctaattg
    34201 tttctatctg atggatatct cacttaaggc tttcttataa atctgtaggg ttttgcctgg
    34261 aagcagacaa ataacccgat aaaacaagga tgagcagatg agcggaaaac cggcagcgcg
    34321 tcagggcgac atgacgcagt atggcggtag cattgttcag ggttcagccg gggtgcgcat
    34381 tggtgccccc accggcgtgg cctgttcggt gtgccccggc ggagtgacgt ccggccatcc
    34441 ggtcaatccc ctgctcggtg caaaggtcct tcccggtgaa accgaccttg ccctgcccgg
    34501 cccgctgccg ttcatcctct cccgcaccta cagcagttac cggacaaaaa cgcccgcgcc
    34561 ggtggggagc ctcggccccg gctggaaaat gcctgcggat atccgcttac agctgcgcga
    34621 taacacactg atactcagtg ataacggcgg cagaagcctg tattttgagc acctgtttcc
    34681 cggtgaggac ggttacagcc gcagcgagtc actgtggctg gtgcgcggcg gcgtcctgag
    34741 actggatgaa ggtcaccggc tggccgcact ctggcaggcg ctgccggaag aactccgctt
    34801 aagtccgcat cgttatctgg cgacaaacag tccgcagggg ccgtggtggc tgctcggctg
    34861 gtgtgagcgg gtgccggaag cggatgaggt gctgcctgcg ccgctgccgt cgtaccgggt
    34921 actgaccggg ctggtggacc gcttcgggcg cacacagacg ttccaccgcg aagccgccgg
    34981 tgaattcagc ggcgaaatca ccggcgtgac ggatggtgcc gggcgtcact tccggctggt
    35041 actgaccacg caggcgcagc gggcagaaga agcccggcag caggccattt ccggcgggac
    35101 ggaaccgtcc gcttttcctg ataccctgcc gggttacacc gaatatggcc gggacaacgg
    35161 catccgtctg tctgccgtgt ggctgacgca cgacccggaa tacccggaga atttacctgc
    35221 cgcgccgctg gtacgctatg gctggacgcc gcgcggcgaa ctggcggtgg tgtatgaccg
    35281 tagtggca
//
LOCUS       DAZFZC010000044        34847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFZC010000044 DAZFZC010000000
VERSION     DAZFZC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.044"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..191)
                     /locus_tag="ACHKXP_002510"
     CDS             complement(<1..191)
                     /locus_tag="ACHKXP_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647491.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETD"
     gene            complement(394..795)
                     /gene="nikR"
                     /locus_tag="ACHKXP_002511"
     CDS             complement(394..795)
                     /gene="nikR"
                     /locus_tag="ACHKXP_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO7647492.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(801..1607)
                     /gene="nikE"
                     /locus_tag="ACHKXP_002512"
     CDS             complement(801..1607)
                     /gene="nikE"
                     /locus_tag="ACHKXP_002512"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO7647493.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1604..2368)
                     /gene="nikD"
                     /locus_tag="ACHKXP_002513"
     CDS             complement(1604..2368)
                     /gene="nikD"
                     /locus_tag="ACHKXP_002513"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO7647494.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2368..3201)
                     /gene="nikC"
                     /locus_tag="ACHKXP_002514"
     CDS             complement(2368..3201)
                     /gene="nikC"
                     /locus_tag="ACHKXP_002514"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO7647495.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3198..4142)
                     /gene="nikB"
                     /locus_tag="ACHKXP_002515"
     CDS             complement(3198..4142)
                     /gene="nikB"
                     /locus_tag="ACHKXP_002515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO7647496.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4142..5716)
                     /gene="nikA"
                     /locus_tag="ACHKXP_002516"
     CDS             complement(4142..5716)
                     /gene="nikA"
                     /locus_tag="ACHKXP_002516"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7647497.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5827..6414)
                     /gene="acpT"
                     /locus_tag="ACHKXP_002517"
     CDS             complement(5827..6414)
                     /gene="acpT"
                     /locus_tag="ACHKXP_002517"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO7647498.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPEQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTAESVQWIDSVN"
     gene            complement(6469..7518)
                     /locus_tag="ACHKXP_002518"
     CDS             complement(6469..7518)
                     /locus_tag="ACHKXP_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7647499.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7650..8867
                     /locus_tag="ACHKXP_002519"
     CDS             7650..8867
                     /locus_tag="ACHKXP_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7647500.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLALPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8871..9428)
                     /gene="dcrB"
                     /locus_tag="ACHKXP_002520"
     CDS             complement(8871..9428)
                     /gene="dcrB"
                     /locus_tag="ACHKXP_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO7647501.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9501..10166)
                     /locus_tag="ACHKXP_002521"
     CDS             complement(9501..10166)
                     /locus_tag="ACHKXP_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO7647502.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10387..10632
                     /gene="tusA"
                     /locus_tag="ACHKXP_002522"
     CDS             10387..10632
                     /gene="tusA"
                     /locus_tag="ACHKXP_002522"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO7647503.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10734..12932)
                     /gene="zntA"
                     /locus_tag="ACHKXP_002523"
     CDS             complement(10734..12932)
                     /gene="zntA"
                     /locus_tag="ACHKXP_002523"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO7647504.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13006..13632)
                     /locus_tag="ACHKXP_002524"
     CDS             complement(13006..13632)
                     /locus_tag="ACHKXP_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO7647505.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLVGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13773..14150
                     /locus_tag="ACHKXP_002525"
     CDS             13773..14150
                     /locus_tag="ACHKXP_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO7647506.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTLSYKGTRFVSFVGEQ"
     gene            complement(14153..14422)
                     /locus_tag="ACHKXP_002526"
     CDS             complement(14153..14422)
                     /locus_tag="ACHKXP_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO7647507.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14412..15008)
                     /gene="rsmD"
                     /locus_tag="ACHKXP_002527"
     CDS             complement(14412..15008)
                     /gene="rsmD"
                     /locus_tag="ACHKXP_002527"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO7647508.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15158..16654
                     /gene="ftsY"
                     /locus_tag="ACHKXP_002528"
     CDS             15158..16654
                     /gene="ftsY"
                     /locus_tag="ACHKXP_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO7647509.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEHAVEEQPQAHTEAEAETFAAEVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16657..17325
                     /gene="ftsE"
                     /locus_tag="ACHKXP_002529"
     CDS             16657..17325
                     /gene="ftsE"
                     /locus_tag="ACHKXP_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO7647510.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDLNLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17318..18376
                     /gene="ftsX"
                     /locus_tag="ACHKXP_002530"
     CDS             17318..18376
                     /gene="ftsX"
                     /locus_tag="ACHKXP_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO7647511.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18621..19475
                     /gene="rpoH"
                     /locus_tag="ACHKXP_002531"
     CDS             18621..19475
                     /gene="rpoH"
                     /locus_tag="ACHKXP_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO7647512.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19746..20849
                     /gene="livJ"
                     /locus_tag="ACHKXP_002532"
     CDS             19746..20849
                     /gene="livJ"
                     /locus_tag="ACHKXP_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO7647513.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARSYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21049..21432)
                     /gene="panM"
                     /locus_tag="ACHKXP_002533"
     CDS             complement(21049..21432)
                     /gene="panM"
                     /locus_tag="ACHKXP_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO7647514.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGAMTAFMQALGFTAQQGGWEKR"
     gene            21856..22965
                     /gene="livK"
                     /locus_tag="ACHKXP_002534"
     CDS             21856..22965
                     /gene="livK"
                     /locus_tag="ACHKXP_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO7647515.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23013..23939
                     /gene="livH"
                     /locus_tag="ACHKXP_002535"
     CDS             23013..23939
                     /gene="livH"
                     /locus_tag="ACHKXP_002535"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO7647516.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23936..25213
                     /gene="livM"
                     /locus_tag="ACHKXP_002536"
     CDS             23936..25213
                     /gene="livM"
                     /locus_tag="ACHKXP_002536"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO7647517.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25210..25977
                     /gene="livG"
                     /locus_tag="ACHKXP_002537"
     CDS             25210..25977
                     /gene="livG"
                     /locus_tag="ACHKXP_002537"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO7647518.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25979..26692
                     /gene="livF"
                     /locus_tag="ACHKXP_002538"
     CDS             25979..26692
                     /gene="livF"
                     /locus_tag="ACHKXP_002538"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO7647519.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            27089..28405
                     /gene="ugpB"
                     /locus_tag="ACHKXP_002539"
     CDS             27089..28405
                     /gene="ugpB"
                     /locus_tag="ACHKXP_002539"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO7647520.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28503..29390
                     /gene="ugpA"
                     /locus_tag="ACHKXP_002540"
     CDS             28503..29390
                     /gene="ugpA"
                     /locus_tag="ACHKXP_002540"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO7647521.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29387..30232
                     /gene="ugpE"
                     /locus_tag="ACHKXP_002541"
     CDS             29387..30232
                     /gene="ugpE"
                     /locus_tag="ACHKXP_002541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO7647522.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30234..31304
                     /gene="ugpC"
                     /locus_tag="ACHKXP_002542"
     CDS             30234..31304
                     /gene="ugpC"
                     /locus_tag="ACHKXP_002542"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO7647523.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPMVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            31301..32044
                     /gene="ugpQ"
                     /locus_tag="ACHKXP_002543"
     CDS             31301..32044
                     /gene="ugpQ"
                     /locus_tag="ACHKXP_002543"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7647524.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32031..32471)
                     /locus_tag="ACHKXP_002544"
     CDS             complement(32031..32471)
                     /locus_tag="ACHKXP_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO7647525.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            32591..34333
                     /gene="ggt"
                     /locus_tag="ACHKXP_002545"
     CDS             32591..34333
                     /gene="ggt"
                     /locus_tag="ACHKXP_002545"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO7647526.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADNLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGLNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(34371..34655)
                     /locus_tag="ACHKXP_002546"
     CDS             complement(34371..34655)
                     /locus_tag="ACHKXP_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO7647527.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         8080 a         9052 c         9755 g         7960 t
ORIGIN      
        1 atgtcggttt caccgggaag gacctttgca ccgagcaggg gattgaccgg atggccggac
       61 gtcactccgc cggggcacac cgaacaggcc acgccggtgg gggcaccaat acgtaccccg
      121 gctgaaccct gaacaatgct accgccatac tgcgtcatgt caccctgacg cgccgccggt
      181 tttccgctca tcgtaaaatc cttttctatt attttaatga cagtatcttc atacgaaaaa
      241 gcgaagaaaa acttgtggtc acaatgaatt gaagattttt aggaatatat ttagaacgtt
      301 acaaaagaga aggttcggat agggtattta gagaggaatc aggtgtgcta gtggcccttt
      361 gttgatcgtt caccaaagga cccaataata gactcaatct tccttcggca agcactgcaa
      421 atgcccgtgc cgcacgccgc gctgggcgat aacgtcatcg gcaaaatgct gcacgtcacc
      481 catgtcacct ttcaacacgg cgatttccag acagtcgtcg tggttgatgt gcacatgcag
      541 cgtggcgacg gagaggtcgt ggtgatgatg ctgggtggag acaatgcggc tggctaagtc
      601 gcgtttttcg tgttcataca catacgacag caccgcgaaa ccttgcgtgc cgtgctgctg
      661 ggtggcctct tgcgccaggg cgctacgcag aatgtcgcgg atagcttcgg aacggttgtt
      721 ataaccacga cgctggctca ggctgtccag cgtctccagt aaatcgtcat caagcgtgat
      781 ggtgactcgt tgcatttgcg ttaaaccttt tctgtggtgc gacggcgcac ggggaatgcg
      841 ggtaataccg cgttttgtag cacacgtccg gcgtcagagg aaaaggttaa tttctctccc
      901 accacctggg tttcgacgat ttgtccgttg tccataacca ttacccgctg gcaaaagcgt
      961 tctaccaggc gtaagtcgtg ggtgatgaac aggcaggcgg tgccaaactg ttgttgtagc
     1021 tttttcagca ggcgaatgac acccgcctgt aacacgagat caaggttaga aacggcttca
     1081 tccagaatca gtagtttcgg ttcgaccgcc agcgcgcgag ccaggcagac gcgctggagc
     1141 tggccgccgc ttaactgcgg tgggcgtttg tcgagaacgc tgtcatcgag atcgaccgcc
     1201 ttcagcattt cgctggcgcg cgccagttgt tcggattttt tcagtgatag caggtggcgc
     1261 atcggttcac gcaggatctc gcgcacggtt ttgcgcggat tcacggcgct gatggagtcc
     1321 tgaaatacca tctgaatatc gcggcggaac gctttacgct gggcgcgatt gagtttcgcc
     1381 agcggttcgc cacgccagct aatattcccc tgcgcgggcg attctaaacc caccagcaac
     1441 cgcgcgaggg tacttttccc gcagccgctg cgccccagca gggcgacggt ttcgccgctt
     1501 ttcagggtca gggaaacgtt attcagcacc gcctgatgtt gatgttttcc gttaaatcca
     1561 ccgtgcgcat agtgatggga aaggccggag atgttaagta aagtcatgat gccagctcca
     1621 taccgtagag ggcgagatga gcggaaacca ggctgcgcgt cactgtatgt ttgggggcgt
     1681 taaacagcgt ttctacatcg ccctgttcga caatcttacc gtcagacatc accgccacgt
     1741 catccgccag acgcgccaca acgcccatat catgggtcac cagcagcatt cccggcgctt
     1801 gtttttgcat aatgctttcc agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt
     1861 cggtggtcgg ttcatcggcg atgataaacg gtgattcaca cagcaccgcc atcgcaatca
     1921 tcatgcgctg caacatgccg ccgctcatct cgaacgggta cagcttcagc acgcgcgcgg
     1981 cgttttccag ccccaccgct tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc
     2041 ctaacgccag gcaggtttcc cgcgcgtggg tgtgcatggt gtgcagtgga ttaaaggcgc
     2101 tgcgcgggtt ctgcatgatg gtggcaattt tgatgccgcg cagggcgcaa ggcgaaaccg
     2161 gtttgccatc ggctaaaatt tcccccgccg tctggcgaac gccagcgggc aaaatgccca
     2221 gcgtcgcggc gcaggttaat gatttcccgc tgccgctacc gccgactaac gccagcacgc
     2281 gcccgcgttg cagggttaac gatacaccgt gtaccagcgg ctgcgcggcc tgtagcgcga
     2341 tattacgtag ttcaatctgt tgcggcatta gtgtgcgtgc tccgtcacca gatgagggtc
     2401 cagatgatcg cgcagtgcgt cacccaccag gttaaaggcc atcacgctga taaacagcgc
     2461 cagccccggc cagaacattt gcagcggctg ggtccagata tactggcgcg cgtcgttaat
     2521 catcacgccc cattcggcgg tcggcgcggt cacaccgagg ccaaggaaag acatccccgc
     2581 gacgtgcagc atcatatggc cgatatccag cgttgccagc accagcagcg aagggatcac
     2641 cgcgcctgcc agatgatcga caaacacccg cacatggccc gcaccggaaa gccgtgacgc
     2701 cagcacaaac tcgcgttggc gtagtgaaat caccaggctg cgcaccatgc gtgcatacca
     2761 cgcccagtgc gacagggcga tggcgataat tacgttggtc agcccggtgc cgagcacgcc
     2821 aaccataaag aacgacagaa tcgaggtcgg gaaggtcata aacatatcgg cgacgcgcat
     2881 ggtggcctga tcaacgcgcc cgccaatcaa cccggcgctg ccgccaataa ccagccctaa
     2941 tgtcagcacc agcagcaggc aggccattac cgaaccgagc gacacgcggg tcgctgccat
     3001 cagccgcgag aaaatatcgc gacctaagtg atcggtgccc agccagtgct gcgcatccgg
     3061 cgaaagcagg cgcgacggca aatcaatcgc ctgggggtca tacggcagcc accactggct
     3121 ggtgagcgca atcagcgcca gcagggcgat aatgatcagc gccaggcgta ccgaccagcg
     3181 ggaagagagg aaaaagttca cgcgtgcgct ccttcatgac gacgaatgcg cgggtccagc
     3241 gcggcgttga gcaaatcgac aatcaaatta cagaccacaa aaaccaccac catcatcagc
     3301 gtaaagcact ggatcaccgg atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg
     3361 acgcccggcc aggcaaagat gttttcgata atcatcgtcc cgccaatcag ttcgccgata
     3421 tgcatcccca cggcggtgat catcggcagc gaggcattgc gcagaatatg gcggcgttcg
     3481 gtctgtttgt cattcaggcc gcgtagccgc gcccaggtga cgtgacgctg accggcgacg
     3541 tccagcatac tggcgcgcag cagtcgcgcg ttaatcgcca gcgacataaa ggcgatggaa
     3601 accgcaggca aaatgatgtg ctgccagccg ccgtagccca tcgcgggtag ccattgcaga
     3661 tacaccgaaa acgccatcac tagcaaaaat gccagccaga agttgggcat cgacacgcca
     3721 agaaacgcga taaagcgcac ggcgaaatcc ggcaaacgat cgcggtggcg cgccgcccag
     3781 ataccgagcg gtacggaagt gagcagaatt aataccagcg ccgcacctgc aagttccagc
     3841 gtggcgggca ggaagttcag catatcgtcc agtaccgggc gttggctggc gaatgagata
     3901 ccaaagtcaa gatgcagcgc cttccacaac caggtaccgt actggacgta cagcggctga
     3961 tccagcccga gcatggtgcg ggtagaggcc agcatctccg gcgtcggcgg caggttagat
     4021 aaacgcagat aatcgagcgc cgggtcgccg gtaccgaggc gcagcatcag aaaaatgatc
     4081 accgaggcgg cgagcaccat cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac
     4141 attaaggttt caccggttta atctgttcga acggaatttc ggtggcgatc ggcgcgtagg
     4201 ggatgttacc cagctccggt tttgatacca ccatcattga gatgtaactg ataggcagat
     4261 aaaccgcctc gtcatgcaga cgggtcagaa tgtcgcgata cagcgcctga cgttgcgttt
     4321 cgtcatgggt cgccagcact tcgccgatct ctttatcaat cagcggtttg tcggctaatc
     4381 cttgctgtgc ctggaagtca gcgtgtgacg gtacgcgcat tgaactgagg aaggcgtgtg
     4441 gatcatatgg cgcgccccag gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac
     4501 gagcatagat actgctctct tcttcgccaa tcagcgagac atctgcgcca atctggcgca
     4561 tatcagcctg aatgatttcc gccatcgatt tgcttaacgc atcggtgccg atgaacgaca
     4621 gttcaatgcg cagcggctga ccatttttct cgcggatgtc tttgcccgca ggcagcgtcc
     4681 aaccggcttt ttccagcaac gctttcgctt tctgcggatc gtactggcgc ggtttcaggc
     4741 cgaggttggc gtagggcaca gaaggggcaa acagggtgtc ggcgacctgc tgggtgccat
     4801 acaacgcgtt atcaatcagc gattttttgt ttaccgcgta attaagagct tcacgtactg
     4861 ccagctcatt ggtgggggct ttggcggtat taagcgccag catcacggtt tcgatcggct
     4921 gtgacagttg ggtatggtaa gccgggttct ggctaaagcg ggcgaaggta tcgagcggta
     4981 ataacccttc gtttccgtac agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg
     5041 tagtcgggtc cgggatgacg ttaaaggtga tctttttaat cgctggcttt tcgccccagt
     5101 agttttcgtt acggacgaag acatcgtact gattcagttt cgattcctgc aaaatccacg
     5161 gtccggtgcc aatcggcgct ttaattccgt tcatggtttc atggttttta aactgcgagg
     5221 gggcgataaa gcggaaaggg cgcggcaggg ccagttcttg caggaaagga tagtaggcgc
     5281 ttttcagggt gatttgcagc tctgttttac tgagtgcttt aacatcaaca atctggtttg
     5341 ccagctccag ccaggcgtga cgttgacggt tatcgagcac tgcgcggaag ttttctgccg
     5401 ccgcctcggc atcgaacggt tcaccgttgg agaatttcac gtcatcacgc agggtgaagg
     5461 tccaggtttt accatcttct gaatgagtcc agctttttgc cagccacggg atcaccgaac
     5521 cgtctgcctg atatttcacc aatggttcat aaaccatgct ctgggcgaac atctggttag
     5581 gcgtgtaaag gtgcgggttt agtggcccga cattcaccgg ccaggcggtg gtgatttcat
     5641 ctggtgcagc ggcatggacg ataaaagacg cacaagccag cagcgcaaat agagtgcggc
     5701 ggagtgtgga gagcatggta accccaatgg attaaaatag atggcggaaa taagtatgac
     5761 gattttaagt attcgtcata ctgattacct gttctggatc aatagatggg cagtcgggtg
     5821 ggcggatcag ttaactgaat cgatccattg cacactctcg gcggtgagcg taaagggggt
     5881 gggggtgcag atcgcaaggc ttaaattttc tagctgacaa tggctgactg acagcgagga
     5941 gtgataggtg ctatctacgc tgacgatttg ccaggcgctg ccgccacgct gtttaacgat
     6001 ggcttctttg cgcgtccaga tgcgccagaa catttccagt tgctgctcag gatgcacggc
     6061 gtccatttca gcgtgttccc cgaggctgaa cacggcgttc gccagccagc gccagttggc
     6121 gcgcgggcga atcacttcga tatcgcagcc gacttcgcct tcatcgctca acagcagagc
     6181 gatatcgtca ccgctatggc ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg
     6241 tttgccttgt tcgccataga tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc
     6301 acgccccgcc agccagcgtt cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccgg
     6361 tggcagtgga gctgcgctta aggtcgaaac tttccccaga actatccgat acatatcagg
     6421 gccaatgttt aatggaaaat gaaagtgcgt atcgtatcac ttgtcgcctc atcccggtaa
     6481 ccgacttttc ggtctgcctg gccccagtaa aatcgccagt ttgctaccgc ctttggtggt
     6541 ttccatccag attttacaca cactggttaa tggcaccgaa agtagcatcc ctaccgggcc
     6601 gagcagccat ccccaaatta acaatgaaag aaataccacc atggtggaca tccccaggcg
     6661 atggcccatc atccgtggct ctaaaatatt gccgatgacc atatggacaa ctaaaaacaa
     6721 tgcgccgacc agaatacatt cgtaaatacc attaaacagc agcacctgaa tcattggcgg
     6781 tacggcggaa attaccgcgc cgatattggg cacgtagtta agcaaaaacg ccagtactgc
     6841 ccacatcagc gcaaactgta cccccatcag ctccagcccc agccagacga tgacacctgt
     6901 ccataaactg agtagcgtct tcaatgcaag atagtgcgaa acgcctttaa gtgcgcggtg
     6961 taatcccgcg atgtgaatct gtggattatt cagcgcaaaa cgcattttgt aagggacgtg
     7021 gcgcacttca aacagcataa aaactacggt catcaccagc aaaagcacgc tcgccattgc
     7081 cccggaaagc ccggtcatta gcgctgtggt gaaggtaacc actttttccg agtccatccg
     7141 ctgcagcatt cgctccggcg acatatgcaa attaagaaaa ggcaacatct cctgcaattt
     7201 aaaaagtttg cgcgtcagct ccttattaaa cttcggcagc atagagataa attcgttaaa
     7261 cgatgccgcc agtacgccga ccagcgcggt tagtgcgatc agcatcacca ccactacaat
     7321 cgtaatggca acggggcgtt gtactccccg acgaataaac caggtgacga gcgggttgag
     7381 gacgatggca aaaaacagcg ccagtaacag ctgcacaatg atatctgccg ctgcgtgaat
     7441 gcccgcgagg atcactacca gcgaggccag cttgagcaga atgtgcatgc ccgttttatc
     7501 gggttgaggg gtttccattg gggcttcctt gtgacttttt gtattaagtg tagcgggagt
     7561 cacgccagca atattctgat tcttactgct gattttcacg tcagcacatg gtaaaaatga
     7621 aacactgttg taaaaatgtg gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa
     7681 acggattgcg cctgaatttg cgcattgtct ccattgtcat gtttaacttc gccagctacc
     7741 tcaccatcgg gttgccgctc gctgtattac cgggctatgt ccatgatgtg atgggcttta
     7801 gcgctttctg ggcaggattg gttatcagcc tgcaatattt cgccaccttg ctgagccgcc
     7861 ctcatgccgg acgttatgcc gatttgctgg gacccaaaaa gattgtcgtc ttcggtttat
     7921 gcggctgctt tttgagcggt ctgggatatc tgacggcagg attaaccgcc agtctgcccg
     7981 tcatcagctt gttattactt tgcctgggac gcgtgatcct tgggattggg caaagttttg
     8041 ccggaacggg atcgaccctg tggggcgttg gcgtggttgg ctcgctgcat atcgggcggg
     8101 tgatttcgtg gaacggcatt gtcacttacg gggcgatggc gatgggtgcg ccgttaggcg
     8161 tcgtgtttta tcactggggc ggcttgcagg cgttagcgtt aatcattatg ggcgtggcgc
     8221 tggtggccat tttgttggcg ctcccgcgtc cgacggtaaa agccagtaaa ggcaaaccgc
     8281 tgccgtttcg cgcggtgctt gggcgcgtct ggctgtacgg tatggcactg gcactggctt
     8341 ccgccggatt tggcgttatc gccaccttta tcacgctgtt ttatgacgct aaaggttggg
     8401 acggtgcggc tttcgcgctg acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat
     8461 tccctaacgg cattaaccgt atcggcggct taaacgtggc gatgatttgc tttagcgttg
     8521 agataatcgg cctgctactg gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg
     8581 tcttactggc gggggcaggg ttttcgctgg tgttcccggc attgggtgta gtggcggtaa
     8641 aagcggttcc gcagcaaaat cagggggcgg cgctggcaac ttacaccgta tttatggatt
     8701 tatcgcttgg cgtgactgga ccactggctg ggctggtgat gagctgggcg ggcgtaccgg
     8761 tgatttatct ggcggcggcg ggactggtcg caatcgcgtt attactgacg tggcgattaa
     8821 aaaaacggcc tccggaacac gtccctgagg ccgcctcatc atcttaaaac ttactgaata
     8881 accagcgtat taatgatgtt ttctgcggtg gtctgcgctt tttgctgatc gtcagcgggc
     8941 agcgtaattt gcatggtcag cagttgatta cccacgttac ccagaataac ggaagagtac
     9001 gccgtctggc ctttcgcgga gataatactg tctaactgct gcattttgtg acctttcagc
     9061 tcaatggctt tattggttac cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc
     9121 agacgcttcg ccagcaccgc cagatcttct tttggatcat cgcccatgat gacgatgact
     9181 gctttctgcc cggtggcgtc ggaccagaca tgcatgttat tggcctgcgt tcccagctta
     9241 ccgctctggt cggtcatatc cgctggcagc gagaaactta acttgccatc aagcaggttg
     9301 acgggattcc cggtagcgtt actttccgcg accgaaccct gcgccgtagc gttagtgtct
     9361 ttatcatcac aggccgcaag ccccataacc agcaggccaa ttccgacata tttaaccaga
     9421 ttgcgcattg acttcttcct ttcgataaac ggccataacg gctcattcat ccatcttatc
     9481 acaactctga taacgaacct ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc
     9541 agtcttttca acagcatatt gagtaatacg ccatacattg gcaggaagaa aacgatactg
     9601 attaacactt tgaaacagta atcgaccagc gcgatttcca tccagtgttc agccataaag
     9661 gcatccgggc tacgccagaa ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg
     9721 aacagtgtgg acgctgtcgg tgccagccac cagcggcgac tctgacgcag gcggttaaaa
     9781 acgtgcacgt cgaggatttg ccccagcgcg taggccatga aactggcggt ggcgatacgg
     9841 gcgacaaaca ggttgaagtg ggcgagtgcg ccgaatccct gccaggaacc catatagaat
     9901 agcgacgaga tgacgtagga gattaataac gcagggatca ttaccgcgaa gataatgcgt
     9961 cgggccagcg gtgcgccaaa aatacgcacg gtcaggtcgg tagcaagaaa aataaacgga
    10021 aagctaaacg cgccccaggt ggtatggaaa cccaaaatgg agacgggaag ctgaaccaga
    10081 tagttactgg aggtgatcac cagcagatga aataacgata accagaacaa cgccttatag
    10141 cgttgagttt gcgagaaaac gttcatattg tacctttttg attaaccatt ggggtgaggg
    10201 aacccaatac gtacgacacg tctgttacct taacataata acgtgcttac ccttttttcg
    10261 agccgccgca tgatactgct ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt
    10321 aacctggttt gcttacggac tcacagggcg taaactagcg ccgttttttt atgtgatgag
    10381 aagaaaatga ccgatctctt ttccagccct gaccacacac tcgacgcact tggcctgcgc
    10441 tgcccggaac ccgtaatgat ggtgcgcaaa actgtgcgca atatgcagcc cggcgaaacg
    10501 ttgctgatta tcgccgacga tccggccact acccgcgata ttcctgggtt ttgtaccttt
    10561 atggaacacg aactggttgc gaaagagacg gatggactgc cttatcgtta tttgattcgt
    10621 aaaggcggtt gatgggggct gattggcttc gatgccgcct tttcccctca ccctaaccct
    10681 ctccccagag gggcgagggg accgatcgcg ctcaatgttg cgatcggttt gccttatctc
    10741 ctgcgcaaca atcttaacgc attcgctgtc accagcaccg tcgcacccgt atctgccagc
    10801 accgccagcc atagtccggt catccctaaa agcgtggtga cgaggaagat ccctttcagt
    10861 cccagcgcaa tggtgatgtt ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc
    10921 atttgcacca ggccgcgcag gtggttatgg gttaatgctg catcagcggt ttccagcgcc
    10981 acgtctgtgc cactgcccat tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg
    11041 ttaataccgt caccgaccat cgccagcggc gcatgttgat tcagctcggt caccgctttg
    11101 actttatctt ccggcaacag gcccgcttta aactccagcc ccagctcccc ggcaattgcc
    11161 gccgctgcgc gtggattatc gccggtgagg atcacccctt tgacgcccag cgcgttcagt
    11221 tcactgatgg cagttgcagc atcggcgcgc agggtatcct gtaacgcaat gacaccaagc
    11281 acgtcatcgt tacgtactac cagcactacc gtttgcccgg cgctttccag ttcgttaatt
    11341 aaaccagcaa atgcatcagc gggatgtttc ccggcagcgc aaatcaatac gcgctcaccg
    11401 ttaacctgcg cttcaatgcc agacccgacc agcgcccgct gtgattcggc ggcgggaatg
    11461 gcgagtgcag caacctgtgc ttcgcgcacg atcgcctgcg ccagtggatg cgtcgcgcct
    11521 tgctcgaccg ccgccgccag tgtcagcagt tcagattcac taatacccgt tgccggatga
    11581 atcgcggtaa cgcgcggttt accgacggtc agcgtaccgg ttttatcaaa cgccacctgg
    11641 gtaacacgac ctagctgctc cagcgccgca ccgcctttaa ttaacgcccc acgacgtgcc
    11701 gctgctgcca gcccggaggt aatcgccgcg ggcgtggaga taactaacgc acacgggcag
    11761 ccaatcagca gcagcgtcag ccctttataa atccactctt gccagctggc ggcaaacagc
    11821 agcggcggca ccagcgttac cagcagggcg acggccataa ttgctggcgt ataaatacgg
    11881 ctgaaacggt cgataaaccg ctcaatgggg gcgcggcgct cttcggcttc ttcgatcagt
    11941 ttcagaatcc ggtcaatggc gctggcaccc ggttctgaca gcacttccag cgttaccaga
    12001 cggtctacgc tggtggctcc tgcaggaact ttatcgcccg ttgcgcgctc caccggaatg
    12061 gattcgccgg tcagggcgct ttcatcaaaa ctggcaaagg gcgagagcag tttaccgtcg
    12121 gcaggcaaac gcccgcccgc cgcaacttca atcacatcgc cagggcgcag gctgttaatc
    12181 gccacctctt cccgctcacc gttacgcagg cgcgtggcgg tttctggttt cagcgccatt
    12241 aacgcgctaa cgccctgacg cgcgcggctg gcggcccagc cttccagtcg ttcaccaatc
    12301 aaaaacagca gcaacaccat cgcagcttca gccgttgcgc caataaacag cgcaccaata
    12361 gcagctacgc tcattaacgt ttcaatggcg aagtaactgc ctgatttgat cagccgtaat
    12421 gcctgacgag caatcgggta cagcccaacc agcgtggtcg cgataaacgc cagttgcccg
    12481 aacggatgat tgaactgctc cagaccccag ctgattgcca tcatcacgat tagcgtaatc
    12541 agcggcagat tctctttcag gcgcgatgct tgtggctcgt cggcggcctg ctcatcacgc
    12601 agggaatagc ccgctttttg caccgcagat tcaacttgtg cacggatgtc attgtcggca
    12661 tcaaccacca gtttttcggt ggcgaacagt acctgcacct gattcacgcc tgcaagctgg
    12721 cgcacggcat tttctacctt gcgcgcacag gcggcgcagt ccatgccgct gactttccag
    12781 ctatagcggg tgccggagac gttttcagag agcgttggcg tgctggaaca tgcgccgtcg
    12841 cagcaacagt cgttggcgtt ctgtaccgtg gttagcggtt tgaacgcggc aaattgaggg
    12901 gctttcttgc cgtgattgtc aggagtcgac atggcatcct ccggttaagt tttttctcat
    12961 taaccgaagg atacactctg gagtcgactc cagagtcaag ttttatcaga gatacagcga
    13021 gcggacgatg aggaagtgac cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg
    13081 gaagcgtcgg cgatagtggc tacccagcca gacaaagtta ctgataaaca gcaacgaagc
    13141 gccgacaaac gcagagagcg ccggagcggt cggacggaag aaccacagtt cacctaccag
    13201 ccacaccatc accagcgtca tgccgataaa cgtgcagata ggccaacgat actcttccag
    13261 ccgcgtccag ataatcgcca gtaacagcgc gcccagaacc agcagtacca acggcagagg
    13321 ccagaagaaa gagagcgtca tctgactggc gaaatagatg gtgtacagca ggtgcgagag
    13381 gaaaaacgcg ccgatggcgt acatcagacg ttgacgtggc aacagggtta gcgcatcgcc
    13441 cagcagtgag gcgcacagcc ctgccagcac cagatagcta atggcgtcga acatcggcgc
    13501 ttgccaggcc agtaacagca ggagaagaag ggttaacggt ttaaacaccc agcgttgcca
    13561 ggttggccca cgatacgatg catccacaga tagccatgcg gaaagacaga cagcgataaa
    13621 cgaccaaagc atcttagttc cttgaattcg ttattttttc gtaagcggaa tgcctacggt
    13681 cttgcttcca gtttagtggc gagggtggac ggatgacaac accataactg gcagggtatg
    13741 cttaattccg cattttccga tgagggataa agatgagtaa accacctctt ttctttattg
    13801 ttatcattgg cttaatcatc gtcgccgcat cgtttcgttt tatgcaacag cgacgggaaa
    13861 aagctgataa tgatatggct ccgctccagc aaaagctggt ggtggtgagc aacaagcggg
    13921 aaaaaccgat taacgatcgc cgttcgcgcc agcaggaagt gactccggca ggcaccagta
    13981 tgcgctatga ggcaagcttc aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg
    14041 acgcccagca gtaccacgcc ctgacagtgg gcgataaagg tacgctgagc tataaaggta
    14101 cgctgagcta taaaggaacg cgctttgtca gctttgtagg cgaacaataa ggttattttt
    14161 taactttaaa tttcttctgc cagaccagca gttcaaacac gccgaaaagg aaaatccgta
    14221 ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa agtggatttc aatagcgcca
    14281 gctgcatacc atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa atattcagcg
    14341 ggcgcgggaa gggatgcacc agattgagga ttaaaaatcc ccaaacgcag agcattaaca
    14401 aacgaccaat attaatcagc atcactttct ccttgtgctt cgcgttgata cagccgataa
    14461 gccacctgac ccgccacttt ttcccgatgt aatgaccagt ttgccggaac agtgggcaga
    14521 ccgttttcga cttcgctttc gacataaatc agggcttcgt cagccagcca gccgttatct
    14581 tccagtaaat ttatcgtctc ttctaacaag ccacggcgga acggtggatc gacaaacacg
    14641 atattatgcg gtgtgccttt ttgcgccagg aatgacattg cgttgctgtt caccacgcgt
    14701 gcattgcctg cttttagtgt cgccagattc ttaattaact gctgagaaac cacgcgatcc
    14761 atctcaatca acgttgcccc cgcagcgtag cgcgataacg cttccagccc cagcgcgccg
    14821 ctcccggcga agcaatccag acattgggcg tcaacaatga ccggagccag ccagttaaac
    14881 aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg aaccgggagt
    14941 ttacggcctc gccactgccc gccaataatg cggatttggc cgctgccgga atgattcggt
    15001 tttttcatga taaattgctc aatccgccag ataacacata cttgcaggcg gtgatgtgaa
    15061 ttaagttaag tgatagacta tttcatcatt tttttagctg ctatgtacat agcgttaacg
    15121 ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg
    15181 ctttttttcc tggctgggct ttggtcaaaa agagcagacc ccggaaaaag agacagaagt
    15241 tcagaatgaa caaccggttg tagaagaaat cgttcaggcg caagagcctg cgaaggcttc
    15301 ggaacacgcc gttgaagagc agccgcaggc gcatactgag gccgaagcgg aaacttttgc
    15361 tgccgaagtt gtggaagtca ctgaacaggt tgctgaaagt gaaaaagcgc agcctgaagc
    15421 ggaagtcgtt gcacagccgg aaccggtcgt agaagaaacg ccggagccag tggctatcga
    15481 acgtgaagag ctgccgttgc cggaagacgt caacgccgaa gaggtttcgc cagaagagtg
    15541 gcaggctgaa gcggaaaccg tagagattgt cgaagcggcg gaagaagaag cggctaaaga
    15601 agaaatcacc gacgaagagc tggaagcaca ggcgctggct gccgaagcgg cagaagaggc
    15661 ggtgatggtg gttcctccgg cagaagaaga gcagccggtg gaagaaatcg ctcaggagca
    15721 ggaaaaaccg accaaagaag gtttcttcgc gcgcctgaaa cgcagcctgt taaaaaccaa
    15781 agaaaatctc ggttccggat ttatcagcct gttccgcggt aaaaaaatcg acgatgatct
    15841 gtttgaagag ctggaagaac agcttttgat cgccgatgtg ggcgtggaaa ccacacgtaa
    15901 aattatcacc aatctgacgg aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct
    15961 ctatggcctg ctgaaagaag agatgggcga gattctggcg aaagtcgatg agccgctgaa
    16021 tgttgaaggt aaaacgccgt tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac
    16081 cacgacgatt ggtaagctgg cacgtcagtt tgagcagcag ggtaaatcgg tgatgctggc
    16141 ggcgggcgat actttccgtg cagcagcggt tgaacagctt caggtctggg gtcagcgcaa
    16201 caatattccg gtgattgccc agcataccgg tgcggattcc gcctctgtta tcttcgacgc
    16261 cattcaggca gctaaagcgc gtaatatcga cgtcctgatt gccgataccg ccggacgcct
    16321 gcagaacaaa tcgcacctga tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact
    16381 cgacgttgaa gcgccgcatg aagttatgct gactatcgat gccagcaccg ggcagaacgc
    16441 ggtaagccag gccaaactgt tccatgaagc cgttggttta accggcatca cgctaacgaa
    16501 actggacggc acggcgaaag gcggggtaat tttctcggtg gctgaccagt ttggtatccc
    16561 tatccgctac attggtgtcg gcgaacgtat tgaggatttg cgtccgttta aggcggacga
    16621 ctttatagag gcactttttg cccgagagga ttaacaatga ttcgctttga acatgtcagc
    16681 aaggcttatc tcggtgggag acaggcgctg cagggcgtta cgttccatat gcagccgggt
    16741 gagatggcgt ttctgaccgg tcattccggc gcagggaaaa gtaccctcct gaagctgatc
    16801 tgtgggattg agcggcccag cgccgggaaa atctggttta gcggccatga catcacgcgt
    16861 ctgaaaaacc gtgaagttcc gtttctgcgc cgccagattg gcatgatttt ccaggatcac
    16921 catctactga tggaccgtac tgtctacgat aacgtggcga tcccgctgat tatcgctggt
    16981 gccagcggtg acgatattcg tcgccgggtg tcggcggcac tggataaagt cgggctactg
    17041 gacaaagcga agaacttccc tattcagctt tcgggcggtg aacaacagcg tgttggcatt
    17101 gcccgcgcgg tggtgaataa gcccgcggta ctgctggcgg acgaaccgac tggtaacctg
    17161 gacgacgcgt tgtcggaagg gattctacgt ctgtttgaag agtttaaccg cgttggggta
    17221 accgtattga tggcaacgca cgacctcaac ctgatttcac ggcgttccta tcgcatgctc
    17281 accctgagcg atggtcactt gcatggaggc gtgggccatg aataagcgcg atgcaatcaa
    17341 tcatattcgg cagtttggcg ggcgtctgga tcgcttccgt aaatcggtcg gcggctcagg
    17401 cgatggcggt cgtaacgcac cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa
    17461 aaccaacgtt ttcaacgaac aggtgcgcta tgccttccac ggcgcattgc aggatctgaa
    17521 aagcaaaccg ttcgccacat ttttaacggt gatggttatc gccatctccc tgacgctgcc
    17581 cagcgtctgt tatatggtgt acaaaaacgt taaccaggcg gcgacgcagt attatccgtc
    17641 accgcaaatc actgtttact tgcaaaaaac gctggacgat gacgccgctg cgggcgtggt
    17701 ggcacagttg caggccgagc aaggcgtgga gaaagtgaac tatctttctc gtgaagacgc
    17761 actgggcgag ttccgtaact ggtctggttt tggtggtgcg ctggatatgc tggaagaaaa
    17821 cccgcttccg gcagtggcgg tggtgatccc gaaactcgat tttcagggga cggaatcact
    17881 caatacgctg cgtgatcgta tcacgcagat taacggcatt gacgaagtgc ggatggatga
    17941 cagctggttt gcccgtctgg cggcgttgac tgggctggtc gggcgcgttt cggcgatgat
    18001 cggcgtattg atggtggcgg ccgtgttcct cgtcatcggc aacagcgtac gtctgagcat
    18061 ctttgctcgc cgtgactcca ttaacgtgca gaaactgatt ggtgcgacgg atggtttcat
    18121 cctgcgcccg ttcctgtacg gtggcgcact gctgggattt tctggcgcat tgttgtcact
    18181 aattttgtca gaaattctgg tactgcgatt gtcatcggcg gttgcggaag tggcacaggt
    18241 tttcggaacg aagtttgata tcaatggttt atcgttcgat gaatgcctgc tgttgctgct
    18301 ggtatgctcg atgattggct gggtggcagc gtggcttgcc acggtacaac atttacgcca
    18361 ctttacgcct gaataataaa agcgtgttat actctttccc tgcaatgggt tccgtagcag
    18421 ggaaagagac cccgttgtct cttcccggta tttcatctct atgtcacatt ttgtgcgtaa
    18481 tttattcaca agcttgcatt gaacttgtgg ataaaatcac ggtctgataa aacagtgaat
    18541 gataacctcg ttgctcttaa gctctggcac agttgttgct accactgaag cgccagaaga
    18601 tatcgattga gaggatttga atgactgaca aaatgcaaag tttagcttta gccccagttg
    18661 gcaacctgga ttcctacatc cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg
    18721 agcgggcgct ggctgaaaag ctgcattacc atggcgatct ggaagcagct aaaacgctga
    18781 tcctgtctca cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc
    18841 cacaggcgga tttgattcag gaaggtaaca tcggcctgat gaaagcagtg cgccgtttta
    18901 acccggaagt gggtgtgcgc ctggtctcct tcgccgttca ctggatcaaa gcagagatcc
    18961 acgaatacgt cctgcgtaac tggcgtattg tcaaagttgc gaccaccaaa gcgcagcgca
    19021 aactgttctt caacctgcgt aaaaccaagc agcgtctggg ctggtttaac caggatgaag
    19081 tcgaaatggt ggcccgtgaa ctgggcgtaa ccagcaaaga cgttcgtgag atggaatcac
    19141 gtatggcggc acaggacatg acctttgacc tgtcttccga cgacgattcc gacagccagc
    19201 cgatggcacc ggtgctctat ctgcaggata aatcatctaa ctttgccgac ggcatcgaag
    19261 atgataactg ggaagagcag gcggcaaacc gtctgaccga cgcgatgcaa ggtctggacg
    19321 agcgtagcca ggacatcatc cgcgcgcgct ggctggacga agacaacaag tccacgttgc
    19381 aggaactggc tgaccgttac ggcgtttccg ctgagcgtgt gcgccagctg gaaaagaacg
    19441 cgatgaaaaa attgcgtgct gctatagaag cgtaatttcc gctattaagc agagaaccct
    19501 ggatgcgagt ccggggtttt tgttttttgg gcctctgtaa taatcaattt cccctccggc
    19561 aaaacgccaa tccccacgca gattgttaat aaactgtcaa aatagctatt ccaatatcat
    19621 aaaaatcggg tatgttttag cagagtatgc tgctaaagca cgggtagtca tgcataaaac
    19681 gaaataaagt gctgaacaac aacatcacaa cacacgtaat aaccagaaga atggggattc
    19741 tcaggatgaa cataaagggt aaagcgttac tggcaggatg tatcgcgctg gcattcagca
    19801 atatggctct ggcagaagat attaaagtcg cggtcgtggg cgcaatgtcc ggtccggtgg
    19861 cccagtacgg tgaccaggag tttaccggcg cagagcaggc ggttgcggat atcaacgcta
    19921 aaggcggcat taaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga
    19981 aacaggcggt tgcggtggcg aacaaagtcg ttaacgacgg cattaaatac gtgattggtc
    20041 acctctgttc ttcatcaacg cagcctgcgt ctgatatcta cgaagacgaa ggcattttaa
    20101 tgatcacccc agcggcaacc gcgccggagc tgaccgcccg tagctatcag ctgatcctgc
    20161 gcaccaccgg cctggactcc gaccaggggc cgacggcggc gaaatatatt cttgagaaag
    20221 tgaaaccgca gcgtattgct atcgttcacg acaaacagca atacggcgaa ggtctggcgc
    20281 gagcggtgca ggacggcctg aagaaaggca atgctaacgt ggtgttcttt gatggtatca
    20341 ccgccgggga aaaagatttc tcaacgctgg tggcgcgtct gaaaaaagag aatatcgact
    20401 tcgtttacta cggcggttat cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg
    20461 cagggctgaa aactcagttt atggggccgg aaggtgtggc taacgtttcg ctgtctaaca
    20521 ttgcgggcga atcagcggaa gggctgctgg tgaccaagcc gaagaactac gatcaggttc
    20581 cggcgaacaa acccattgtt gatgcgatca aagcgaaaaa acaggaccca agcggcgcat
    20641 tcgtttggac cacctacgcc gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg
    20701 atccggctga aatcgccaaa tacctgaaag cgaactccgt ggataccgta atgggaccgc
    20761 tgacgtggga tgagaaaggc gacctgaaag gctttgagtt cggtgtattt gactggcacg
    20821 ccaacggcac ggcgaccgat gcgaagtaat cattaatcgg catctttggg ttgccgccaa
    20881 attgctaata tcgagtacgt tgcttcatgc cggatgcggc gtaaacgcct tatccggcct
    20941 acaagatcca aagaaatcag taaattgcag cacacattgt aggcctgata agcgtagcgc
    21001 atcaggcaat ttctcgttgg tcatcacttt tgaaatcaaa cttgacgatt aacgcttctc
    21061 ccagccgccc tgttgtgccg taaatcccag tgcctgcata aacgccgtca tcgcaccgcg
    21121 atcttccacg cctgcatccg ccatccacca gcatgaaacg ccaggattgt tacgcaaaac
    21181 ctcttccagc agatattgcc ccacaccgcg acggcgggtg acttcccgca cgcgtagaga
    21241 atccagtgct ccctcggtgc cgcttaaggt tacccgcacg gccgcaagca gacgctcgtt
    21301 aaaacgcgcg gcgtagatac ggtggttatc gtcaacctgt aaagaggaag gggaatactc
    21361 cggccagatc ttttgcaggt caatccggtc ctggtcgcta aagttttcta atcgaatgat
    21421 ggtcagcttc atgggtaacc cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat
    21481 ttaatcggag gctttctctt ttttatttct tttggcaggt gattaatttt ttaacagcaa
    21541 taattacaaa gttaaaacat tagagaatga aaaataccca gcataatccc ctgaatggta
    21601 gtgaattatt ccgccctttg tgccgttatt ttatgctgac aacggcgctt ttttctgttt
    21661 atctatcaat aaattcagaa tattatctgt tcttaatcga ctgaaaaata gggattttaa
    21721 tcgctattat cacaaaatac tgcgctaaac ccttaatcaa acaggcaaaa acagtgcagt
    21781 ataaaaaaag aacagtctga tttgttaaca cataaaaaca aagcaacaca acatcacgaa
    21841 tggggatttt tgactatgaa acggaatgcg aaaactatca tcgcagggat gattgcactg
    21901 gcaatttcac acaccgctat ggctgacgat attaaagtcg ccgttgtcgg cgcgatgtcc
    21961 ggcccgattg cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc aattaaagac
    22021 attaatgcca aagggggaat taagggcgat aagctggttg gcgtggaata tgacgacgcc
    22081 tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg ttaatgacgg cattaaatac
    22141 gttattggtc atctgtgttc ttcttctact caacctgcgt cagatatcta tgaagatgaa
    22201 ggtattctga tgatctcgcc gggagcgacc aatccggaac tgacccaacg cggttatcaa
    22261 cacatcatgc gtactgccgg gctggactct tcccaggggc caacggcggc aaaatacatt
    22321 cttgagacgg tgaagcccca gcgcatcgcc atcattcacg acaaacaaca gtatggcgaa
    22381 gggctggcgc gttcggtgca ggacgggctg aaagcggcta acgccaacgt cgtcttcttc
    22441 gacggcatta ccgccgggga gaaagatttc tccgcgctga tcgcccgcct gaaaaaagaa
    22501 aacatcgact tcgtttacta cggcggttac tacccggaaa tggggcagat gctgcgccag
    22561 gcccgttccg ttggcctgaa aactcagttt atggggccgg aaggtgtggg taacgcatca
    22621 ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg tcactatgcc aaaacgctat
    22681 gaccaggatc cggcaaacca gggcatcgtt gatgcgctga aagcagacaa gaaagatccg
    22741 tccgggcctt atgtctggat cacctacgcg gcggtgcaat ctctggcgac tgcccttgag
    22801 cgtactggca gcgatgagcc gctggcgctg gtgaaagatt taaaagctaa cggtgcaaac
    22861 accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc ttaagggatt tgattttggt
    22921 gtcttccagt ggcacgccga cggttcatcc acggcagcca agtgatcatc ccaccgcccg
    22981 taaaatgcgg gcgggtttag aaaggttacc ttatgtctga gcagtttttg tatttcttgc
    23041 agcagatgtt taacggcgtc acgctgggca gtacctacgc gctgatagcc atcggctaca
    23101 ccatggttta cggcattatc ggcatgatca acttcgccca cggcgaggtt tatatgattg
    23161 gcagctacgt ctcatttatg atcatcgccg cgctgatgat gatgggcatt gataccggct
    23221 ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat tgccagcgcc tacggctgga
    23281 gtatcgaacg ggtggcttac cgcccggtgc gtaattctaa gcgcctgatt gcgcttattt
    23341 ccgcaatcgg tatgtccatc ttcctgcaaa actacgtcag cctgaccgaa ggttcgcgcg
    23401 atgtggcgct gcccagcctg tttaacggtc agtgggtggt gggccatagc gaaaacttct
    23461 ctgcctctat taccaccatg caggcggtga tctggattgt caccttcctc gccatgctgg
    23521 cgctgacgat tttcattcgc tattcccgta tggggcgcgc ctgtcgtgcc tgcgcggaag
    23581 atctgaaaat ggcgagcctg cttggcatca acaccgaccg ggtgattgct ttgacctttg
    23641 ttattggcgc ggcgatggcg gcagtggctg gcgtactgct cggtcagttc tatggcgtca
    23701 ttaaccccta tatcggcttt atggccggga tgaaagcctt taccgctgcg gtgctcggtg
    23761 ggattggcag cattccaggg gcgatgattg gcgggctgat cttggggatt gcggaggcgc
    23821 tctcttctgc ctatctgagt acggaatata aggatgtggt ttcatttgcc ctgctgattc
    23881 tggtgctgct ggtgatgccg accggtattc tgggtcgccc ggaggtagag aaagtatgaa
    23941 accgatgcat attgcaatgg cgctgctctc tgccgcgatg ttctttgtcc tcgctggcgt
    24001 ctttatgggc gtgcaactgg agctggatgg caccaaactg gtggtcgaca gcgcttcgga
    24061 tgtccgttgg cagtgggtgt ttatcggcac ggcggtggtc tttttctttc agcttttgcg
    24121 acctgctttc cagaaagggt tgaaaagtgt ttccggaccg aagtttattc tgcccgccat
    24181 tgatggctcc acggtgaagc agaaactgtt cctcgtggcg ctgctggtgc ttgcggtggc
    24241 gtggccgttt atggtgtcac gtgggacggt ggatatcgcc actctgacca tgatctacat
    24301 tatcctcggt ctcgggctga atgtggttgt tggtctttct ggtctgctgg tgctggggta
    24361 cggcggtttt tacgccatcg gcgcttacac ctttgcgctg ctcaatcact attacggctt
    24421 gggcttctgg acttgtctgc cgattgccgg gttaatggca gcggcggcgg gcttcctgct
    24481 cggctttccg gtgctgcgtc ttcgtggtga ttatctggcg atcgttaccc ttggcttcgg
    24541 cgaaattgtg cgcatattgc tgctcaataa caccgaaatt accggcggcc cgaacggaat
    24601 cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc agccgtaccg ctcgtgaagg
    24661 cggctgggac acgttcagta atttctttgg cctgaaatac gatccctccg atcgcgtcat
    24721 tttcctgtat ctggtggcgt tgctgctggt ggtgctaagc ctgtttgtca ttaaccgcct
    24781 gctgcggatg ccgctggggc gtgcgtggga agcattgcgt gaagatgaaa ttgcctgccg
    24841 ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgcc tttaccatca gcgccgcgtt
    24901 tgccggtttt gccggaacgc tgtttgcggc gcgtcagggc tttgtcagcc cggaatcctt
    24961 cacctttgcc gaatcggcgt ttgtgctggc gatagtggtg ctcggcggta tgggctcgca
    25021 atttgcggtg attctggcgg caattttgct ggtggtgtcg cgcgaattga tgcgtgattt
    25081 caacgaatac agcatgttaa tgctcggtgg tttgatggtg ctgatgatga tctggcgtcc
    25141 gcagggcttg ctgcccatga cgcgcccgca actgaagctg aaaaacggcg cagcgaaagg
    25201 agagcaggca tgagtcagcc attattatct gttaacggtc tgatgatgcg cttcggcggc
    25261 ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc aggaaatcgt ctcgttaatc
    25321 ggccctaacg gtgccggaaa aaccacggtt tttaactgcc tgaccggttt ctacaaaccc
    25381 accggcggca ccattttact gcgcgatcag catcttgaag gtttaccggg gcagcaaatt
    25441 gcccgcatgg gcgtggtgcg caccttccag catgtgcgtc tgttccgtga aatgacggta
    25501 attgaaaacc tgctggtggc gcagcatcag caactgaaaa ccgggctgtt ctctggcctg
    25561 ttgaaaacgc cttctttccg tcgcgcccag agcgaagcgc tcgaccgcgc cgccacctgg
    25621 cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg cgagtaacct ggcctatggt
    25681 gaccaacgcc gtctggagat tgcccgttgc atggtgacgc agccggagat tttaatgctc
    25741 gacgaaccgg cggcaggtct taacccgaaa gagacgaaag agctggatga gctgattgcc
    25801 gaactgcgca atcatcacaa caccactatc ttgttgattg aacacgatat gaagctggtg
    25861 atggggattt cagaccgaat ttacgtggtc aatcagggga cgccactggc aaacggtacg
    25921 ccggagcaaa tccgtaataa cccggacgtg atccgtgcct atttaggtga ggcataagat
    25981 ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac tacggcaaaa tccaggcgct
    26041 gcatgaggtg agcttgcata tcaatcaggg cgagattgtc acgctgattg gcgcgaacgg
    26101 ggcggggaaa accaccttgc ttggtacgtt atgcggcgat ccgcgcgcca ccagcgggcg
    26161 aattgtgttt gatgataaag acattaccga ctggcagacg gcgaaaatca tgcgcgaagc
    26221 ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg atgacggtgg aagagaacct
    26281 ggcgatgggc ggcttctttg ccgaacgtga ccagtttcag gagcgcataa agtgggtgta
    26341 tgaactgttt ccacgtctgc atgagcgccg tgttcagcgg gcgggcacca tgtccggcgg
    26401 tgaacagcag atgctggcga ttggtcgcgc gctgatgagc aatccgcgtt tgctgctact
    26461 tgatgagcca tcgctcggtc ttgcgccgat tatcatccag caaattttcg acaccatcga
    26521 gcagctgcgc gagcagggga tgactatctt cctcgtcgag cagaacgcca accaggcgct
    26581 aaagctggcg gatcgcggct acgtgctgga aaacggccat gtggtgctct ccgataccgg
    26641 cgacgcgctg ctggcgaacg aagcagtgag aagtgcgtat ttaggcgggt aataacacgt
    26701 tgattgataa ggagtcaaaa gactcctttg agacaggtga caaatgtaaa attgcctgat
    26761 gcgctgcgct tatcaggcct actgggtgag ttgcaatata ttgaatttgc acgatcttgt
    26821 aggccggata aggcgtttac gccgcatctg gcatgaaacg atgagtaatc tgtagagttt
    26881 gattcagacc ttctattttt ccgcttatcc gtgccccatc tcccattttc cctcacccac
    26941 gccgtcaccg ccttgtcatc tttctgacac cttactatct tacaaatgta acaaaaaagt
    27001 tatttttctg taattcgagc atgtcatgtt accccgcgag cataaaacgc gtgaattcgc
    27061 gcattcggta caacaagaga gataaacgat gaaaccgtta cattatacag cttcagcact
    27121 ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg acgaccattc cgttctggca
    27181 ttctatggaa ggggaactgg gtaaagaggt ggattctctg gcccaacgtt ttaacgccga
    27241 aaatccggat tacaaaattg taccgaccta taaaggcaac tacgaacaga atttaagcgc
    27301 ggggattgcc gcatttcgta ccggcaacgc tccggctatt ttgcaggttt atgaagttgg
    27361 caccgccacc atgatggcat cgaaagccat taaaccggta tatgacgtgt ttaaagaggc
    27421 ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt tccggttact actccgacag
    27481 caaaacgggc cacttactct cccagccatt caacagctcg acccccgttc tctattacaa
    27541 caaagacgcc ttcaagaaag caggattaga cccggaacaa ccgccgaaaa cctggcagga
    27601 tctggcggac tatgccgcga aactgaaagc ctccggcatg aagtgcggct acgccagcgg
    27661 ctggcagggc tggatccaac tggaaaactt tagcgcctgg aacggtctgc cgtttgccag
    27721 caaaaacaac ggctttgacg gcacggacgc ggtgctggag ttcaacaagc cggagcaggt
    27781 gaaacacatc gccatgctcg aagagatgaa caagaagggc gacttcagct acgtcggtcg
    27841 taaggatgaa tccaccgaga agttctataa cggtgattgc gcgatgacca ccgcctcttc
    27901 cggttctctt gccaacattc gcgagtacgc caaattcaac tacggcgtag gcatgatgcc
    27961 ttacgacgcc gatgcgaaag atgcgccaca aaacgccatt atcggcggag ccagcctgtg
    28021 ggtgatgcag ggtaaagata aagaaacgta taccggtgtg gcgaagttcc tcgatttcct
    28081 cgcgaagcca gaaaacgctg ccgagtggca tcagaaaacc ggttatctgc caatcaccaa
    28141 agcagcgtat gacctgaccc gtgagcaggg cttttatgag aaaaacccag gggcggatac
    28201 cgcgacgcgt cagatgctga ataagccgcc gttgccgttc accaaagggc tgcgtctggg
    28261 caacatgccg cagatccgcg tgattgtgga tgaagagctg gagagcgtgt ggaccggtaa
    28321 gaagacacca cagcaggcac tggataccgc cgttgagcgt ggaaatcagt tgctgcgccg
    28381 ctttgagaaa tcgacgaagt cttaatcagt gtaatgtcgg atgcgtttcg cttatctgac
    28441 ctggcatcgc gtgtaggccg gataagcgaa gcgcatccgg cacagttcag gaattaaccg
    28501 taatgtcatc atcccgtccg gtgttccgct cgcgctggct gccttatctg ctggtcgcgc
    28561 cgcagctcat catcaccgtt atctttttta tctggcctgc gggcgaagcg ttgtggtact
    28621 cgctacaaag cgtcgatccg tttggtttct ccagccagtt tgtcggcctg gataacttcg
    28681 tcacgctgtt ccatgacagc tactatctcg actccttctg gacgacgata aaattcagca
    28741 cctttgtcac cgtcagcggt ttgctggtat cgctgttctt tgcggcgctg gtggagtaca
    28801 tcgtgcgcgg cagccgtttc tatcaaacct taatgttgct gccttacgcc gtggctcccg
    28861 ccgttgccgc cgtattgtgg atcttcctgt ttaaccccgg tcgcgggctg atcacccatt
    28921 ttctcgcgga gttcggctac gactggaacc acgcgcaaaa cagcggtcag gcaatgtttc
    28981 tggtggtgtt tgcctcagta tggaagcaaa tcagctacaa cttcctgttc ttctatgccg
    29041 cgctgcaatc cattccccgt tcgttgatcg aagccgcagc catcgacggt gcagggccga
    29101 ttcgccgctt ctttaagatt gcgctgccgc ttatcgcccc ggtgagtttc ttcctgctgg
    29161 tagtgaacct ggtgtatgcc ttcttcgaca ccttcccggt gatcgacgcc gccacgtccg
    29221 gcgggccggt tcaggccacc acgacgctga tttataagat ctaccgcgaa ggttttaccg
    29281 gactggatct ggcttcgtct gccgcacagt cggtggtgtt gatgttcctc gtcatcgtgc
    29341 tgacggtggt gcagttccgc tatgttgaaa gcaaggtgcg ttaccaatga ttgagaaccg
    29401 tccgtggctg acgatattca gccataccat gctgatcctc gggatcgcgg tgatcctctt
    29461 cccgctgtac gtggcgtttg tcgcggcgac gctggataaa caggccgtct atgccgcgcc
    29521 gatgacgctc atccccggca cacatctgct ggaaaacatc cacaacatct gggtgaacgg
    29581 ggtaggcacg aatagcgcgc cgttctggcg gatgttgctt aacagctttg tgatggcgtt
    29641 cagcattacg ctcggcaaaa ttaccgtctc gatgctctcg gcatttgcca ttgtctggtt
    29701 tcgttttccg ctacgtaacc tcttcttctg gatgattttt atcaccctga tgctgccggt
    29761 tgaagtacgt atcttcccga cggtggaagt catcgccaac ctgcagatgc tcgacagcta
    29821 cgccggttta acgctgccgc tgatggcctc ggcgaccgct actttcctgt tccgccagtt
    29881 ctttatgacg ctgccggatg agctggtgga agccgcgcgg atcgacggcg catcgccaat
    29941 gcgcttcttt tgcgacatcg tttttccgct ctccaaaact aatctggcgg cgctgtttgt
    30001 gatcaccttt atctacggct ggaatcagta tttgtggccg ttgttgatta ttaccgatgt
    30061 ggatctcggc accaccgtgg cagggatcaa agggatgatc gctacaggcg aaggcaccac
    30121 ggaatggaac tcagtgatgg cggcgatgtt gttaacgctt atccctccgg tggtgattgt
    30181 tttagtgatg cagcgtgcct tcgtgcgcgg cctggtcgat agtgagaaat aagatggcag
    30241 gactgaaatt acaggcagta accaaaagct gggatggtaa aacccaggtc attaaaccgc
    30301 tgacccttga tgtggcggat ggcgaattta tcgtgatggt cgggccgtct ggctgcggga
    30361 aatcgacgct gctgcgcatg gttgccgggc tggagcgggt gacagaaggc gatatctgga
    30421 tcaacgacca gcgcgtgact gaaatggagc caaaagatcg cgggattgcg atggtgttcc
    30481 agaactacgc gctttatccg catatgagtg tcgaagaaaa catggcgtgg gggctgaaaa
    30541 ttcgcggcat gggcaagcag caaattgccg agcgcgttaa agaagcggcg cgcattctgg
    30601 agctggacgg tctgctcaaa cgtcgcccgc gcgagctttc cggcggtcag cgccagcgtg
    30661 tggcgatggg ccgcgcgatt gtgcgcgatc cggcggtgtt cctgtttgat gagccgctct
    30721 ctaacctcga tgccaagctg cgcgtgcaga tgcgtcttga actgcaacag ttgcaccgtc
    30781 gcctgaaaac gacttcactc tacgttactc acgatcaggt tgaagcgatg acgctcgccc
    30841 agcgagtaat ggtgatgaac ggcggtgttg ccgaacagat tggcacacca gttgaagtct
    30901 acgaaaagcc cgccagcctg tttgtagcga gttttatcgg cagtccggcg atgaacctgc
    30961 tgacaggccg cgtgaataac gaaggcacgc atttcgaact ggacggcggt attgagctgc
    31021 cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat gactctcggc attcgcccgg
    31081 aacatattgc gctaagctcg caggcagaag gcggcgtacc gatggtgatg gacacgctgg
    31141 agatcctcgg cgcagataac ctggcgcacg gacgctgggg cgagcagaag ctggtggtgc
    31201 gactggcgca tcaggagcgc ccgacggcag gcagcacgct gtggctgcat ctggcggaaa
    31261 atcagctgca tctttttgat ggtgaaacag gacaacgagt atgagtaact ggccttatcc
    31321 ccgcatcgtc gctcatcgtg gcggcggtaa gctggccccg gaaaacaccc tggcggcaat
    31381 cgacgtcggg gcaaaatacg gtcataagat gatcgaattt gacgcgaagt tatcgaaaga
    31441 tggcgagatc ttcctgctcc atgacgacaa tctcgaacgc accagcaacg gctggggcgt
    31501 cgcgggtgaa ctgaactggc aggatttact gcgcgtggat gcgggcagtt ggtacagcaa
    31561 agcgtttaaa ggtgagccgc taccgttgct ttcgcaggtg gcggaacgct gtcgtgaaca
    31621 cgggatgatg gcgaatatcg aaatcaaacc caccactggc actggaccat taacgggcaa
    31681 aatggtggcg ctggcggcac gccaactgtg ggccggtatg acgccgccgc tgctgtcatc
    31741 gtttgagatt gatgctttag aagccgcaca acaggcagta ccggaactgc cgcgcggttt
    31801 gttgctggat gagtggcgcg acgactggcg cgaactgacc gcgcggctgg gctgcgtctc
    31861 tattcatctc aatcataagt tactcgataa agtgcgagtg atgcagttga aagacgccgg
    31921 actgcggatt ctggtttata ccgtcaataa accccagcgc gcggcagagt tgctgcgctg
    31981 gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt ggtccgaact ttacggccca
    32041 atagttttca acggaatgtc aggctgcggc gtgctgggct gattcagcat gtcgccgttt
    32101 ctcctctccg gcagcatatg ctgctgattc aacgaactat ccggattacg gttgctgttt
    32161 aacatcccgc cgttggtgtt gggcagcatt tgctgccggg cgggatttcg ctcccccggc
    32221 tgtgactgca acacccgctg agaattattg tttatctggt tttctaaatg ctgctgttgc
    32281 aactgagttt gcgttttcag ttgctgattc agcatccctt tttgctggat ttgctgagtc
    32341 tgcatctggg tttgcatccg ctgctggctg ggtatctgat accccggctg gttagggttg
    32401 ttcagagtat taatgggctg tgcaaagccg acaaacggca ggagtgccgt aagaatcaga
    32461 agtcgtttca tcgcgtatcc tcctctgaag atatccttta agtttactcg cttcccgaca
    32521 aaacgatgat taattcagag ttatatacca ggcttagctg gggttgcccc ttaatctctg
    32581 gagaataacg atgataaaac cgacgttttt acgccgggtg gccattgctg ctctgctctc
    32641 aggaagttgt tttagcgccg ccgccgcgcc tcctgcgccg cccgtctcgt atggtgtgga
    32701 ggaagatgtc ttccacccgg tacgcgcgaa acagggaatg gtagcgtctg tggacgccac
    32761 tgccactcag gtgggggtgg atatcctcaa ggagggcggg aatgccgttg atgccgccgt
    32821 ggcggtgggc tacgcgctgg cggtaacgca tccgcaggca gggaatctgg gcggtggtgg
    32881 ttttatgtta atccgctcga aaaatggcaa taccacggct atcgatttcc gcgaaatggc
    32941 acccgccaaa gcgacccgcg atatgttcct cgatgatcag ggcaacccgg acagcaaaaa
    33001 atcactcact tcgcatctgg cttccggcac accgggtacg gtagcaggtt tctcgctggc
    33061 gctggataaa tacggcacca tgccgctgaa caaagtcgtg cagcccgcgt ttaaactggc
    33121 acgcgatggt tttatcgtta acgacgcgct ggctgacaat ctcaaaacct acggtagcga
    33181 agtgttgccg aatcacgaaa acagtaaagc tatcttctgg aaagagggcg agccgctgaa
    33241 aaagggcgac acgctggtgc aggcgaacct ggcaaagagc ctggagatga ttgctgaaaa
    33301 cggcccggac gaattctata aaggcacgat tgcggaacag atcgcccagg agatgcagaa
    33361 aaacggtggc ttgatcacta aagaagattt agcggcctat aaagcggtcg aacgcactcc
    33421 gataagcggc gattatcgcg ggtatcaggt ttactccatg ccaccgccat cctccggcgg
    33481 gatccatatc gtacaaatcc tcaatattct ggaaaacttc gatatgaaga aatacggctt
    33541 tggcagcgcc gatgcgatgc aaatcatggc agaagcggag aaatacgcct acgccgaccg
    33601 ctcggaatat cttggcgacc cggattttgt caaagtaccg tggcaggcgc tgaccaataa
    33661 agcctatgcc aaatctattg ccgatcaaat tgatatcaat aaagcgaagc catccagcga
    33721 aattcgcccc ggcaagcttg cgccttatga gagtaatcaa actacccatt actcagtggt
    33781 ggataaagat ggtaacgcgg tggcggtgac ctatacgctg aacaccacct tcggtacggg
    33841 cattgtcgcg ggcgagagcg gtattctgct taataaccag atggatgatt tctccgccaa
    33901 accgggcgta ccgaacgttt acgggctggt gggcggtgat gccaacgccg tcgggccgaa
    33961 caaacgcccg ctgtcgtcga tgtcgccgac cattgtggtg aaagacggta aaacctggct
    34021 ggttaccggt agcccaggcg gtagccggat catcactaca gtgctgcaaa tggtggtgaa
    34081 tagcatcgat tatggcttga acgtcgccga agcgaccaat gcgccgcgtt tccaccatca
    34141 gtggttgccg gacgagctgc gtgtcgaaaa agggtttagc ccggatacgc tcaagctgct
    34201 ggaagcaaaa ggtcagaaag tggcgctgaa agaggcgatg ggcagtacac aaagcattat
    34261 ggttgggccg gacggtgagt tgtacggcgc atccgacccg cgctcggtgg atgatttaac
    34321 ggcggggtac taaggttagc ggccctcttc gtgggaagag ggctattttg tcagggcaag
    34381 ccgaaggtag ccttttttat ttcgtaatcc tgtagatatt cttccagcgg ttttgccgtt
    34441 cccagagaat gaatttcacc actgtcttta tcaataataa aaggtgcgtt accagctaag
    34501 cgcgcggcct catctccagt ttcgagaaat tctcgtgctt cgaaacagaa ataccagccc
    34561 tggctaaagc gtccatgtag agtaatgacg accgggagat ctgcatcatc aaggtaatgg
    34621 ttcgctttcg cgaatgcgtc gtgataagta atcataatta taataaatat tattgttgag
    34681 tgttatatta ttattcctgt gtgatacatt gagcaaagac gcgttcatct tcgtcaaaga
    34741 tattattaaa tgctggtttt gaaaaaagtt catcggataa gatatagtca cgaaataagt
    34801 atctgctaat attttatgat ttctttttca gaacgtcgaa cgggatt
//
LOCUS       DAZFZC010000045        33775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFZC010000045 DAZFZC010000000
VERSION     DAZFZC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.045"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            233..1291
                     /gene="citC"
                     /locus_tag="ACHKXP_002547"
     CDS             233..1291
                     /gene="citC"
                     /locus_tag="ACHKXP_002547"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO7647528.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1306..1602
                     /gene="citD"
                     /locus_tag="ACHKXP_002548"
     CDS             1306..1602
                     /gene="citD"
                     /locus_tag="ACHKXP_002548"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO7647529.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1599..2507
                     /gene="citE"
                     /locus_tag="ACHKXP_002549"
     CDS             1599..2507
                     /gene="citE"
                     /locus_tag="ACHKXP_002549"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO7647530.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2518..4050
                     /gene="citF"
                     /locus_tag="ACHKXP_002550"
     CDS             2518..4050
                     /gene="citF"
                     /locus_tag="ACHKXP_002550"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO7647531.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4054..4605
                     /gene="citX"
                     /locus_tag="ACHKXP_002551"
     CDS             4054..4605
                     /gene="citX"
                     /locus_tag="ACHKXP_002551"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO7647532.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEELLNDVDACNVN"
     gene            4580..5458
                     /gene="citG"
                     /locus_tag="ACHKXP_002552"
     CDS             4580..5458
                     /gene="citG"
                     /locus_tag="ACHKXP_002552"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO7647533.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVLNHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5509..6972
                     /gene="citT"
                     /locus_tag="ACHKXP_002553"
     CDS             5509..6972
                     /gene="citT"
                     /locus_tag="ACHKXP_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO7647534.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7086..7892
                     /gene="rna"
                     /locus_tag="ACHKXP_002554"
     CDS             7086..7892
                     /gene="rna"
                     /locus_tag="ACHKXP_002554"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO7647535.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8121..8531
                     /gene="rnk"
                     /locus_tag="ACHKXP_002555"
     CDS             8121..8531
                     /gene="rnk"
                     /locus_tag="ACHKXP_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO7647536.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8669..8758
                     /gene="yldA"
                     /locus_tag="ACHKXP_002556"
     CDS             8669..8758
                     /gene="yldA"
                     /locus_tag="ACHKXP_002556"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO7647537.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8762..10000)
                     /gene="ybdR"
                     /locus_tag="ACHKXP_002557"
     CDS             complement(8762..10000)
                     /gene="ybdR"
                     /locus_tag="ACHKXP_002557"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO7647538.1"
                     /translation="MKALTYHGPHHVQVENVPDPGVEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLHFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10221..10649
                     /gene="uspG"
                     /locus_tag="ACHKXP_002558"
     CDS             10221..10649
                     /gene="uspG"
                     /locus_tag="ACHKXP_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO7647539.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAQERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10770..12335)
                     /gene="ahpF"
                     /locus_tag="ACHKXP_002559"
     CDS             complement(10770..12335)
                     /gene="ahpF"
                     /locus_tag="ACHKXP_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO7647540.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12580..13143)
                     /gene="ahpC"
                     /locus_tag="ACHKXP_002560"
     CDS             complement(12580..13143)
                     /gene="ahpC"
                     /locus_tag="ACHKXP_002560"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO7647541.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13515..14261
                     /gene="dsbG"
                     /locus_tag="ACHKXP_002561"
     CDS             13515..14261
                     /gene="dsbG"
                     /locus_tag="ACHKXP_002561"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO7647542.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14470..15372
                     /gene="ybdO"
                     /locus_tag="ACHKXP_002562"
     CDS             14470..15372
                     /gene="ybdO"
                     /locus_tag="ACHKXP_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7647543.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEDPLPSIKPY
                     ISYNKSSLNNLVFSRFIDRLNECF"
     gene            15519..16739
                     /gene="ybdN"
                     /locus_tag="ACHKXP_002563"
     CDS             15519..16739
                     /gene="ybdN"
                     /locus_tag="ACHKXP_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7647544.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKSKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16712..17341
                     /gene="ybdM"
                     /locus_tag="ACHKXP_002564"
     CDS             16712..17341
                     /gene="ybdM"
                     /locus_tag="ACHKXP_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO7647545.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDERIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNDQLVPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17342..18502)
                     /gene="ybdL"
                     /locus_tag="ACHKXP_002565"
     CDS             complement(17342..18502)
                     /gene="ybdL"
                     /locus_tag="ACHKXP_002565"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO7647546.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAHHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18611..19699
                     /gene="hcxA"
                     /locus_tag="ACHKXP_002566"
     CDS             18611..19699
                     /gene="hcxA"
                     /locus_tag="ACHKXP_002566"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO7647547.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFKKVESFKA"
     gene            complement(19709..19906)
                     /gene="ybdD"
                     /locus_tag="ACHKXP_002567"
     CDS             complement(19709..19906)
                     /gene="ybdD"
                     /locus_tag="ACHKXP_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7647548.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(20089..22194)
                     /gene="cstA"
                     /locus_tag="ACHKXP_002568"
     CDS             complement(20089..22194)
                     /gene="cstA"
                     /locus_tag="ACHKXP_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO7647549.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22375..22788)
                     /gene="entH"
                     /locus_tag="ACHKXP_002569"
     CDS             complement(22375..22788)
                     /gene="entH"
                     /locus_tag="ACHKXP_002569"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO7647550.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22791..23537)
                     /gene="entA"
                     /locus_tag="ACHKXP_002570"
     CDS             complement(22791..23537)
                     /gene="entA"
                     /locus_tag="ACHKXP_002570"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO7647551.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23537..24394)
                     /gene="entB"
                     /locus_tag="ACHKXP_002571"
     CDS             complement(23537..24394)
                     /gene="entB"
                     /locus_tag="ACHKXP_002571"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO7647552.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24408..26018)
                     /gene="entE"
                     /locus_tag="ACHKXP_002572"
     CDS             complement(24408..26018)
                     /gene="entE"
                     /locus_tag="ACHKXP_002572"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO7647553.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQVADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(26028..27203)
                     /gene="entC"
                     /locus_tag="ACHKXP_002573"
     CDS             complement(26028..27203)
                     /gene="entC"
                     /locus_tag="ACHKXP_002573"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO7647554.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27392..28348
                     /gene="fepB"
                     /locus_tag="ACHKXP_002574"
     CDS             27392..28348
                     /gene="fepB"
                     /locus_tag="ACHKXP_002574"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7647555.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVANERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(28352..29602)
                     /gene="entS"
                     /locus_tag="ACHKXP_002575"
     CDS             complement(28352..29602)
                     /gene="entS"
                     /locus_tag="ACHKXP_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO7647556.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQRPPQVTASDG"
     gene            29713..30717
                     /gene="fepD"
                     /locus_tag="ACHKXP_002576"
     CDS             29713..30717
                     /gene="fepD"
                     /locus_tag="ACHKXP_002576"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO7647557.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAIVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30714..31706
                     /gene="fepG"
                     /locus_tag="ACHKXP_002577"
     CDS             30714..31706
                     /gene="fepG"
                     /locus_tag="ACHKXP_002577"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO7647558.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31703..32518
                     /gene="fepC"
                     /locus_tag="ACHKXP_002578"
     CDS             31703..32518
                     /gene="fepC"
                     /locus_tag="ACHKXP_002578"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7647559.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32515..33648)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKXP_002579"
     CDS             complement(32515..33648)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKXP_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO7647560.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKSFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
BASE COUNT         8098 a         9210 c         8524 g         7943 t
ORIGIN      
        1 attaattaaa aaacttaaaa ccattaaata aataaaaaac cactaactct atgtgaaata
       61 aatcaaaatt tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata
      121 cctcgatcac ccacctttct cccaagtgaa aataaaaagt tatcagtttg caacattgaa
      181 caacattcgt tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg
      241 caatgatatt ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca
      301 attcctgcat gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac
      361 ccgcgatgaa aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc
      421 tatcagtgaa tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa
      481 cctcgcctat gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc
      541 gctgttccgc cagtgcggtt tttccacgct gacctgcgta cccggcgtga tggtgctgat
      601 ggaaaacagc gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc
      661 agggaacaag attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta
      721 tctgattcaa caggctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga
      781 ttcttcacgc ttcccctatg aagaccggct ggatctggtg ttaaaaggca ccgccgatat
      841 tccacgcctg actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg
      901 ctacttcatt aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat
      961 tttccgtcag tacctcgctc ccgcactggg tgtaactcac cgctttgtcg gtactgaacc
     1021 cttttgtcgc gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac
     1081 tatctccgca ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc
     1141 gatatccgct tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc
     1201 gctggtccct gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga
     1261 cgcggcagct cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag
     1321 cccgccgttg caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat
     1381 acgcaggata tcgacctgca aatcaacagc agcgttgaga aacagtttgg cgatgcaatt
     1441 cgcaccacca ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc
     1501 gatgacaaag gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc
     1561 gccagcggta tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac
     1621 aacgtaaaac tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg
     1681 tcagcaactc cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtag
     1741 cattgcgtga aaaagacacc gcccgccgca tggtttacca cgcgctgcaa catccgctgt
     1801 atcgcgatat tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgtcaacg
     1861 acctggaagc cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg
     1921 ctcaggatgt tctggatatc gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg
     1981 aacccggcag caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag
     2041 tggaaatcgc tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg
     2101 tgcgcaacct gcgtacagaa cgctccccgg aaggaactga actgctgttc gctcgctgct
     2161 ccattttgca ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg
     2221 ctaacaacga agctggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg
     2281 gcaaatcgct gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc
     2341 agaaagaagt ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag
     2401 gcctcggcgt ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc
     2461 gtctggtgct ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg
     2521 acgcagaaaa ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct
     2581 gaatgcgatc ttgccgcttt ccagaactcg ccgaaacaaa cctatcaggc tgaaaaagcg
     2641 cgcgatcgca aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac
     2701 ggcatgacgg tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg
     2761 atggacgtca tcgcgaagat gggctttaaa aacctgaccc tggcgtccag ctccctgagt
     2821 gattgccatg cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc
     2881 tccggcctgc gtggtccact ggcggaagag atctcccgtg gtctgctggc agaaccggtg
     2941 cagatccact ctcacggcgg acgtgtgcat ctggtacaga gcggcgaact gaatatcgac
     3001 gtggctttcc tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt
     3061 aaagcctgct gcggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc
     3121 gtgatgctta ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat
     3181 caggttgatt tgatcgtcaa agttgaccgc gttggcgatg ctgcaaaaat cggcgctggc
     3241 gcgacccgta tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg
     3301 attgtcaact ctggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgca
     3361 tcgctggcgg taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac
     3421 ttcgcccttg gcggtattac cgcgacgatg gttgacctgc acgaaaaagg tctgatccgc
     3481 aaactgctgg atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc
     3541 aatcacatcg aaatcagcgc caaccagtac gctaactggg gttcgaaagg cgcatcggtt
     3601 gatcgtctcg acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac
     3661 gtgctgaccg gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg
     3721 attgcctctg cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgactctg
     3781 gtggataacg tgctgacctg catcacccca ggctccagtg tcgatattct ggtcacagac
     3841 cacggtatcg cagttaaccc ggcacgtccg gaactggcag aacgtctgca ggaagcgggc
     3901 attaaagtgg tttccattga gtggctgcgc gaacgtgcgc gtctgctgac cggtgaacca
     3961 cagccgattg aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg
     4021 atcgatgttg tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc
     4081 caccatgcgg tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa
     4141 cacgcctggc tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg
     4201 ccgattaaag acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt
     4261 gccttagcca caaagcaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc
     4321 gggccggagg gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt
     4381 gagcttgaac atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa
     4441 ggcgaaattc tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag
     4501 caaagcgcag ctgtttgtgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc
     4561 atggaggagc tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc
     4621 gacgtcatta atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa
     4681 tctgtcaccg aaaccaggcc ttgtggatcg cattaactgc ggtgcgcaca aagatatggc
     4741 gctggaagat ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga
     4801 atttggtgcc tgtagtgcag aaatggcacc agaagcggta ctccacggat tacgcccaat
     4861 tggtatggct tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg
     4921 cagcattttt tctttagggc tgctatgtgc ggcaattggc cgtttgcttc agctcaacca
     4981 gccggtaacg ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga
     5041 tcgcgaactg cgtaccaata attcacaact gacggcaggt caacggttgt accagcagct
     5101 tggccttacc ggcgcacgcg gtgaagccga agcgggttat ccactagtgc tcaaccacgc
     5161 cctgccgcat tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga
     5221 taccttgctc ctactgatgg cgatcaacgg cgataccaac gttgcatcgc gcggtggcga
     5281 ggggggcctg cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg
     5341 aacccccgcc gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct
     5401 cagtccaggc ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt
     5461 atttaagcac ttgataaatt tggaaatatt aattttcgga gaacccgtat gtctttagca
     5521 aaagataata tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc
     5581 cctgtccccg acggtatgcc accacaggca tggcattact tcgctgtgtt tgtggcaatg
     5641 attgtcggca tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact
     5701 atttgcgtta ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg
     5761 tttaatgcgc aaaaacaggc gctgaaatgg ggtctggctg gtttttccag caccacggta
     5821 tggctggtat ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt
     5881 cgcattgccc ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg
     5941 attgtcatta tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg
     6001 ggtacggttt ttccggtcat taaaaacctg ccgccgctgt ttaaatcatt ccccaacgat
     6061 ccgtccgcgc gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg
     6121 agttcgtcca tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc
     6181 aaaattgccg gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg
     6241 gttatcttgc ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca
     6301 cacagtgaag aagtggcaac ctgggcgggt gatgaactaa aaaccatggg tgcgctgaca
     6361 cgcagagagt ggacgctgat tggccttgta ttgctcagct taggtttgtg ggtatttggc
     6421 agtgaagtca ttaatgctac tgcggttggt ctgctggcag tttcgctaat gctggctctg
     6481 cacgttgtgc cgtggaaaga cattacccgc tataacagcg catggaacac gctggtcaac
     6541 ctggcaactc tggttgtgat ggctaacggc ctgactcgtt ctggttttat tgactggttc
     6601 gccggtacca tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg
     6661 gttctggtgt tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc
     6721 atgctgccgg ttattctggc cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg
     6781 tgtatcctgc tggtgctgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt
     6841 cctggggtga ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc
     6901 gcaatcttcg gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc
     6961 atgtggaact aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggtaagccc
     7021 acctggcggg cttttttatg atttaataga tagtattcag tgtatgtatg agttccacac
     7081 ccattatgaa agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttct
     7141 cttctgccaa cgccgtagcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc
     7201 tggccctctc ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac
     7261 gagatgaatg tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg
     7321 gtctgtggcc aggattgcct aaatcggtcg ctgcccgtgg tgttgatgaa cgccgctgga
     7381 tgcgcttcgg ttgcgctact cgcccaatcc cgaatctacc agaagcgcgc gccagccgga
     7441 tgtgttcatc gccggaaacc ggattatcac tggaaacggc cgctaaacta agtgaagtca
     7501 tgccaggagc tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct
     7561 gctttggttt tgatccggac gcatacttcg gtactatggt acgcctgaat caagaaatta
     7621 aagagagcga agccggaaaa ttccttgcgg ataattacgg taaaacagtg agccgccgtg
     7681 actttgacgc cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa
     7741 cgtgccaggg taaccctgcg tatttgactg aaattcagat ctcgatcaaa gctgacgcca
     7801 tcaacgctcc gctttctgca aactcattct tgccacaacc tcacccaggt aactgtggca
     7861 aaacctttgt gattgataaa gcgggttatt aatcctctga tttacaaaac acgctaattc
     7921 gtcttccata accatgacga ttaagaatta tacataatgc gctgcattga ggaattccct
     7981 gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa
     8041 ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt
     8101 accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg
     8161 aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt
     8221 taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg
     8281 tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca
     8341 cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg
     8401 taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg
     8461 gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact
     8521 acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca
     8581 tcctctttac gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa
     8641 ttattccgtt tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt
     8701 tctgattatg gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg
     8761 atcatattgt tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct
     8821 ctgcgctttg tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga
     8881 aaatctcata tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg
     8941 gtttcagtaa tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg
     9001 tctgtcccat tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa
     9061 taaatccagc gtagacgccg ggtacgctaa caatgccgcc acgcctgacc gccgcaatac
     9121 actggcgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg
     9181 tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac
     9241 ctgccgtttg ttcaataatt gactgtgccg gatcgctgtc ttcatcaaaa ttaatcggga
     9301 tcgcgccgta gcggtcggcg gcgaaatgca agcggtaggg atgatgatca acaacaaaaa
     9361 tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccaca ggaccagcac
     9421 catagactgc aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg
     9481 ttggcagaat atcagaaagg aaaagcgctt tatcatctga aagcaaaggc ggtactttaa
     9541 acggccccac attcccttta gggacgcgga catattccgc ctgcccacca ggaacgccgc
     9601 catacaggtg actataacca aacaatgccg ctggagctgg tatctgtttt ttattgagcg
     9661 cagcgccttt acccgcattg gtattttcgc aggcggcata ttgttgcaat cgacagaaaa
     9721 aacagtcgcc acaagcaatg acgaacggaa ttaccactcg gtcgcctttt tgcaaatttt
     9781 ttacgtcctt tccggtttca actacttccc ccataaattc atgaccaaaa atatcgccat
     9841 gtttaacctg aggtattttg cctcgataaa gatggaggtc agagccacag atcgccgttg
     9901 ccgtaatacg cagaataata tcatctgcct gttcaacgcc cggatcggga acattttcta
     9961 cctgaacgtg atgtgggccg tgataagtca atgctttcat gcaacctcca gagacagttc
    10021 aaaaaagagt cagtctttaa gggtagctat cacagcggga aaacgggcgg taccacaggt
    10081 tttgggagaa ttcctggtgt tcagtgagaa tatattcatc acagaaacag ttcgtaagga
    10141 aaaagaacca gacaaatatc agggataaat attataatca tggcgaatga tttgttcatg
    10201 attaacaggg agaaagggtt atgtataaga caatcattat gccagttgat gtatttgaaa
    10261 tggaattgag cgacaaagct gttcgccacg ctgaattcct cgcccaggat gacggagtta
    10321 ttcatctact tcacgtacta cccgggtcag ccagcctgag cctgcaccgt tttgccgctg
    10381 atgtgcgtcg ttttgaagag catctgcaac atgaagcaca agaacgtctg caaacgatgg
    10441 tcagccactt caccatcgat ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc
    10501 gggatgaagt caatgagttg gcagaagaac tgggggctga tgttgtagtt attggttctc
    10561 gcaacccatc gatttcgacc catctgttag gttctaacgc ctcgagcgtg atccgccacg
    10621 ccaatctgcc ggtgctggtt gtgcgttaat tgccagaata agtatcccgc cctgcccgga
    10681 gccatcccgg cggggctttt taatgccata aaaaagccgc caggtttgac cccggcggct
    10741 aagcaattgc aggtgaatct tacttcttct tatgcagttt tggtgcgaat caggtagtca
    10801 aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgatgatctg cttgtacgga
    10861 accgtcgtac agtcacccgc tgcgaacacg cctttcacgt tggtttcgca tttcgcatca
    10921 atgataatct cgcccatgcg gttacgttcg actgcgcctt cgagccagtt ggtgttcggc
    10981 agcagaccaa tctggacgaa aataccggcc agttcgatgt tgtgaatatc gccgctgaca
    11041 cgatctcgat attccagacc aacgactttg ctgccgtcgc ctttcacttc cgtggtttgc
    11101 gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag aacctggtcg
    11161 gctttcattt ctggcgcaaa ttccagcagc gttacgtgct caacgatacc cgccaggtca
    11221 attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt acctttaaac
    11281 agcgggccgt cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg atcttcgccc
    11341 ggaacgttca tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg tgctttcagt
    11401 accgcgccag aagctgtttc aatctgatgc agaccacctt caactgctgc tgggatcagt
    11461 ttgctggcgc tctggctgtc gatcacatca acgtcgtatt catcaacgtg aactttcagt
    11521 gcgcctgcca gcttctgccc ttcagtcttc ggtacagaaa tgtagttttc gatatcaacg
    11581 gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat gcctttacgt
    11641 gcggagtaaa ttgctgccgc tgcacccgcc gggccggaac cgacgattaa tacgtcataa
    11701 gcatcacgct tgttcaactc ttctgccgca cgtttttccg cgccagtatc aattttggca
    11761 acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac gaacactgcc
    11821 ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc gtcaattgca
    11881 gtgtgcttga tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac cacgtccggg
    11941 cagttgtggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc aatatggcga
    12001 atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca cagcaacgcc
    12061 agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg cccctggttg
    12121 gaacctgggt tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt atcttcttta
    12181 aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat ttctgccgat
    12241 ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa
    12301 gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc tttaaacatc
    12361 atcatgcaag cggcctctgc ggctacctgg atgcagcttg catcatggtg cggggaagaa
    12421 aatcgggtgc cgcagcaccc gaagaattag gtgaatttcc gaagtatttc gcgtcagagc
    12481 aaggcggcta gcctgtgaat ccccgggagc ttacacaagt aagtgaccgg ggtgagcggg
    12541 cgacgccaac gccgctatgg cgtgaaagac gaaggaaatt tagattttac caaccaggtc
    12601 cagagacgga gccagagttg cttcaccttc tttccattta gccgggcaaa cttcacctgg
    12661 gtgagaagct acgtactgtg ctgctttgat tttacgcagc aggtcagacg cgtcacggcc
    12721 aatgccttca gcggtaactt cgattgcctg gatgataccc tgcgggtcaa caacgaaggt
    12781 cgcacggtca gccagacctt catcttcacg catgttgtcg aagttacggg tcagggcgcc
    12841 agtcgggtcg ccgatcatcg catatttgat tttagcgatg gtttcagagc tgctgtgcca
    12901 tgctttgtgg gtgaagtgag tatcggtaga tactgcgtat acgtctacgc ccagtttctg
    12961 cagttcttcg tagtggtcag caacgtcacc cagttcggtc gggcatacga aagtaaagtc
    13021 agccgggtag aagaagaaga cgctccagcg gccttcggta tctttttcgg tgatttcgat
    13081 gaattcgccg tttttgaatg cctggttttt aaaaggttta attttggtgt taatcaagga
    13141 catctatact tcctccgtgt tttcgatgag atgtaaggta accaattttt gccgattcgg
    13201 ctaatgcgtt tccattatca aatcgataag ttttacctta caaccttcgt aagacaactt
    13261 tgtgaacttt gcaccgccag cggcgataaa aagtaaaatg gccccctttc caggtgaccg
    13321 tgtaatctga actacccttt ggtatcttca gtgcctgcct gcaggctgag gtgttgccct
    13381 tacaaatgca acaacgacat ggattacaac accctcataa acaaagggca atcacctgat
    13441 ctaagctctt acctatgaca gtgataggtt atgcctttta ctcgactttt gcactgactg
    13501 aaaaggacaa attaatgtta aaaaagatac ttttactggc tctgcttcct gcaatcgcct
    13561 tcgcagagga acttcctgct ccagtaaaag cgattgaaaa acagggcatt acaatcatca
    13621 aaacattcga tgcccccgga ggaatgaaag gttatctcgg aaagtatcag gatatgggcg
    13681 tcaccatcta cctgactcca gatggtaagc acgctatctc tggttacatg tacaacgaga
    13741 aaggtgaaaa cctgagtaac acacttatcg aaaaagaaat ttacgcacca gccggacgcg
    13801 aaatgtggca acggatggaa caatcccact ggctcctcga cggtaaaaaa gatgcgccgg
    13861 tcattgtcta cgtcttcgcc gatccgttct gcccatattg taaacagttc tggcagcagg
    13921 cgcgcccgtg ggtagattct ggcaaagtgc aattaagaac attgttggtt ggggttatca
    13981 agccagaaag cccggcgaca gcagcggcaa ttcttgcctc caaagatccc gcaaaaacct
    14041 ggcaacaata tgaagcctct ggtggcaagc ttaagctaaa cgtgcctgca aacgtaagta
    14101 cagagcaaat gaaagtgtta agtgacaatg agaaactgat ggacgatctg ggggcaaatg
    14161 tcacgccggc tatctattac atgagtaagg aaaatacgct acaacaggcc gtggggttgc
    14221 ccgatcagaa aacgcttaat atcattatgg ggaataaata aaaggtaatt catggatggt
    14281 tgaactatat ccctgctttt caaccatcct aaaaaagcat gaattgctcg ctctgcccta
    14341 agtggctgtg ctattttaga aaaacttaat cctgagttaa gcaaaaaaac ataatcaata
    14401 aaaatatata cgcaaaaaac attgattaag tgaatatatc atggaagaaa aatataaccg
    14461 gagtagtgta tggccaatct ctacgacttg aaaaagttcg acttaaatct tcttgtcata
    14521 tttgagtgta tttaccagca tttaagcatt agcaaagctg ctgaatcact atatatcacc
    14581 ccatcagccg tgagccagtc gttacaacgc ttacgtgcgc aatttaatga ccctttattc
    14641 attcgctcag gaaaaggtat cgcgccgacg acaacagggt taaatctgca tcaccatctt
    14701 gagaaaaatc tcagaggcct tgaacaaaca attaatatag tcaataagtc cgaactaaag
    14761 aaaaacttta ttatctacgg ccctcagctc atttcttgct ctaataatag tatgctgatc
    14821 cgctgcctac ggcaagattc ctctgtcgag attgagtgcc atgatatcct catgtcagca
    14881 gagaatgctg aagaactgtt ggttcaccgc aaagccgatc tggtgatcac ccaaatgcca
    14941 gtgatcagcc gttccgtcat ttgcatgcca ttgcatacga ttcgcaatac tctaatttgt
    15001 agtaataggc atccacgaat tactgacaat agcacttatg aacaaattat ggcagaagag
    15061 ttcacccaat taatatctaa atcagcaggg gttgatgata ttcaaatgga aatagacgag
    15121 agatttatga atcgcaaaat ctcatttcgt ggttcctctt tgctgacaat cattaatagc
    15181 attgcagtta cagatttact gggaatagtc ccctatgaac tttataattc ctaccgtgac
    15241 tttcttaatt taaaagagat aaaactggaa gatccgttac cttcaataaa gccttacatt
    15301 tcctataata aatcttcttt aaacaatctg gtattctcac gatttattga ccgcctaaat
    15361 gagtgttttt gatatataac ttcagaaagc tactataact ttcacctatt tattcacggc
    15421 gcgaatgaaa aataaaacac tctgctttac agtgtttact ttataattaa cagacttatc
    15481 tcactgatca ccctgtaacg ttcagagagc gtcttcagat gtctatttat aaaattcctc
    15541 ttccgctcaa tattctggaa gctgcacgag agcgtattac ctggacactc aacaccctac
    15601 cccgcgtttg tgtctcattt tctggcggca aagactctgg tttaatgctg catctgacag
    15661 ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt gtttatcgac tgggaggcgc
    15721 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
    15781 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
    15841 ccgaatggca gtgctgggaa cctgatgtcg aatgggtacg tcaacctccc caagatgcga
    15901 taaccgaccc tgactttttc tccttttacc agccaggcat gaccttcgaa caatttgtac
    15961 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
    16021 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
    16081 acgataaacc ctggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
    16141 acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaagt ctctgcaacc
    16201 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
    16261 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
    16321 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
    16381 acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac tggcaagaat
    16441 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
    16501 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
    16561 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
    16621 acgattactg gtgtcgggca ttatcattta gccccacaaa atcgaagaac tatcagcgtt
    16681 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
    16741 gcaggattta acgcaatttc tcgccagctt gccggaagac gaacgcatca aagcgattaa
    16801 tgaaatccgc atggcgatcc atcaggtaag tccttttcgg gaagaaccgg tagattgtgt
    16861 tttgtgggtc aaaaatgatc agcttgtgcc gaatgattac aacccaaaca acgtggcacc
    16921 acccgaaaaa aaactgctga aaaaatccat tgaaatagat ggttttaccc aacccattgt
    16981 ggtcacgcat acggataaaa atgccctgga aatcgtggat ggttttcacc gccatgaaat
    17041 tggtaaaggc tcatcatcgt taaaattacg cttaaaaggt tatttgccgg tgacctgtct
    17101 ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt cataatcgcg ctcgtggacg
    17161 ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt agccagttgg gatgggacga
    17221 taataaaatt ggcaaagagc tgggaatgga cagcgacgaa gtgctgcgcc tgaagcaaat
    17281 taacggcctg caagagttgt ttgccgaccg tcaatattcc cgcgcctgga cggttaaata
    17341 gctaaagctg gcgcaggcgt tcagctgctg ccagcaacgt cgattccttc ttggcaaaac
    17401 agagacgaat cagtttatgt gggaagggat cggcgcaaaa caccgacagc ggaatcgccg
    17461 ctacgccgtg ctcctgcgtc agccactggc aaaactcaac atcatccagg gtagaaaccg
    17521 cgctgtaatc caccagcaaa aagtatgtac cttcacacgg taaaatctcc agccggcttt
    17581 catttaaagc attcaccaga atatcgcgct tctggcgata aaagtccggt aacgcaagat
    17641 aatgctcagg ttctgcacgt agcatatcag caagcgccag ctgtgccggg gtattcaccg
    17701 aaaaggtcag atactgatgt accttgcgaa tttcggcgct gatgggcgct ggcgcaacac
    17761 aataacccac tttccagccg gtcatatgat aggtcttgcc aaatgaagaa accgccactg
    17821 cccgctcacg cagctgcgga tgcgccagca cactggcatg gccctgttgt gaaaagttga
    17881 tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg cccggcgatc gcctgccaca
    17941 aagcggcgaa atcagcctgc tgccagacag ttgcactggg gttatgcgga gtgttgagga
    18001 tcaccagtct ggtgcgctcg cttaataatg cggcaaattc ctgccagtca acgcgaaaat
    18061 gcggtggttg cagtgccata cgcttcacta ttcccccaga aagcgcgatg gcgggggcgt
    18121 aactgtcata gctgggatca aaacaaatca cttcatcgcc attgcgcacc agtgcggtaa
    18181 tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt gatatcgcta tcggcatctg
    18241 gttgatagcc atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag gcctgcacgc
    18301 cggtcatggg cgcgtattgg tttgccccct gtgcaacgtg gtgcgccagc cgctcctgta
    18361 aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc tggtgttgct
    18421 gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt ttgctttgtg
    18481 gaatcagagg gttatttgtc atttttattg tacctgacga agttgcgtgt tgaaggcact
    18541 ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa aacaataagg
    18601 acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta actacttttc
    18661 acatccagga agtttcaatc acctgcacga ttttttcact gatgaacaac tttctcgcgc
    18721 ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa accaaacttc cgccagcgtt
    18781 tggactgcca ggggcaaagc atattttgtt tcgcggtcat tgcagcgaaa gcgatgtaca
    18841 acaactggcg gctgagtccg gtgacgaccg cagcgtggtg attggcgtcg gtggcggtgc
    18901 actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctgccgtttg ttgccgttcc
    18961 gacgatcgcc gccacctgcg ccgcctggac accgctctcc gtctggtata atgatgccgg
    19021 acaggcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg tggaaccgga
    19081 gattatcctc aatgcaccgc aacaatatct gctggcgggg atcggtgaca cgctggcgaa
    19141 atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa ccgtgcgact
    19201 ggggatcaat aatgcgcaag ccattcgcga cgtcttgtta aacagtagcg aacaggcgct
    19261 gagcgatcag caaaatcaac agttaacgca atcattttgc gatgtggtgg atgctattat
    19321 tgctggtggt gggatggttg gtggtctggg cgatcgtttt acgcgtgtgg cggcagctca
    19381 tgccgtgcat aacggtctga ccgtgctgcc gcaaaccgag aagtttctcc acggcaccaa
    19441 agtcgcctac ggaattctgg tgcaaagcgc cttgctgggt caggatgatg tgctggcgca
    19501 attaactgga gcgtatcagc gttttcatct gccgactaca ctggcggagc tggaagtgga
    19561 tatcaataat caggcggaga tcgacaaagt gattgcccac accctgcgtc cggtggagtc
    19621 cattcattac ctgccagtca cgctgacacc agatacgttg cgtgcagcgt tcaaaaaagt
    19681 ggaatcgttt aaagcctgac gtacaaattt agcagcagcg cgcgccgcct tttccaccgt
    19741 agcgcgcgtc ctgccgctca cggaaaaact cttcgtaggt catcggcgtt tgatcgggat
    19801 ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc atcagcttcg
    19861 ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcttg gcctccgcta
    19921 accatgttgc tgaaagagtg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatgct
    19981 atcaacgcgc tcggtcagtc ccctcgcccc accggggaga gggttagggt gagagggaac
    20041 cctgggcact aagccaacat tcgccaacat ccccccctca ctctgacttt agtgtgcgcc
    20101 ttttgcctgc gccacgatct cctcgacatt ttccggcatt ggttcatacg gcgtttcttt
    20161 cgccgttggc ttcggatctt tcaatgccgc aagtgccgtc ttaatcgaga acagtgccag
    20221 aaccaccacg accaccataa agaagatggt taacccggca tccagacggt tgttgaacac
    20281 cagttgcgcc agctgtgact cagtatactg cgacggaata ttgccgctgt cgatcattgc
    20341 ctggaactta ttagcaatgg ccaggaagcc gactttcgca tccgggctaa acgctttctg
    20401 ccagcctgcg gtcagggtac aaatcagcag ccaggccgtt ggtaccagcg ccacccaggc
    20461 gtaacgttga cgtttcatct tgaacaacac cacggcacag agcatcagcg ccatccctgc
    20521 cagcatctgg ttggcaatac caaacagcgg ccacagagtg ttaatgccgc ccagcggatc
    20581 gaccacgccc tgatggagga agtagcccca cgccagtacg cacagcgctg ttgccagcag
    20641 gttagcaggc agtgaatcgg tccgcttcag gccaggagac accacgccca gcagatcctg
    20701 caacataaag cgcgcagcac gcgtacctgc atccaccgcc gtcagaataa acagtgcttc
    20761 aaacaaaatg gcgaagtgat accagaacgc cacatccatc atgccgccca gcgcgccgtg
    20821 cagaatgtag gccatcccca ccgccagcgt cggcgcaccg cccgcacggg aaatgatcga
    20881 ctgttcaccc acttcgctgg caatctggtt tagcgtatct ggtgtaatgc taaagcccca
    20941 gctactcacc acctgcgcgg cagaagcgac cacatccgcc gtccctgccg gagccagcac
    21001 cgccatcggg ctgttcatgg caaaatacac gcccggatcg atgatacagg cggaaaccag
    21061 cgccataatc gccacgaagg attccattaa catcccaccg tagccgataa agcacgcctg
    21121 cccttcgttc gccagcatct tcggcgtggt cccagaagag atcagcgcat ggaagccaga
    21181 caccgcgcca caggcgatgg tgataaacag gaacgggaac aggttaccgg tccataccgg
    21241 gccagtgcca tcgacaaatt tggtcagcgc aggcatggtc agcgtcgggc gcataatcaa
    21301 aatgcctacc gccagaccga cgatagtccc gattttcagg aaggtagaga ggtagtcacg
    21361 cggggccagc agtaaccaca ccggcagcac cgccgccaca aaaccgtaac ccaccagcat
    21421 ccaggtcagc tgcacgccgg taaagtcaaa gtacggtgcc caggtcggac tttctgccac
    21481 ccagccgcca gagataatgg cgaaaatcag gaacaccagg ccaatcactg acacttcgcc
    21541 aatgcggccc ggacgcagat agcgcaggta gattcccata aaaatcgcca gcggaatggt
    21601 gaacgcaacg gtatacgttc cccacgggct atgagtcagg gctttcacca cgatcatcgc
    21661 cagtactgca aggataatga ccatgatcat aaagcaggct accagcgcaa tcaccccggc
    21721 agttggcccc atctcttctt tgaccagctc acccagcgat cgaccgtcac ggcgcgtaga
    21781 aacaaacagc accatgaaat cctgcaccgc accagcgaga accaccccgg cgagcagcca
    21841 gatcatccct ggcaggtaac ccatttgcgc cgccagtacc ggcccaacca acggacctgc
    21901 tccggcaatg gccgcaaaat ggtgaccgaa cagcactttc ttgtccgtcg gcacatagtc
    21961 cagcccgtcg ttatggcgca ccgctggcgt catacgcgtc gggtcaaccg ccagcacatt
    22021 tttggcgata tacagcccat aaaaacggta agcgatcaga tagatacaga ccgacgccac
    22081 cacaatccac agcgcgttga tctgttcccc acgatttaaa gcaatgtatc ccagagcaaa
    22141 tgctcccatt acagagagca ctgtccagac gaggtatttc cctgatttgt tcatagttgt
    22201 tatccgtgtg cgtgtccata gagatgttac attttgtttc tataacatct ttgtacgatt
    22261 taacaacacg gtaaccatgt aaatcggagt tgaaaccaat atttaactta acaatgttaa
    22321 ctcgatcact ccgttaaccg agttttcctg caatctcatt taattctgtc gggttcatcc
    22381 caaaactgcc gtacccagcc gacaagtgca gcaacgccgc ccctgttcat cgaaaacgac
    22441 gatttcccag ctctgatttt gccgaccaag atgcagcggc tggcagacgc cgcgtacctt
    22501 tccctcagac accgggcgat ggtgtgttgc attaagttct gtgcctacca cacactgtcc
    22561 gtcgcgggtc atcataaatc cggccatcga tcccagcgtt tccgccagcg ccgccgacgc
    22621 gccgccatgt agtaaaccga acggctgatg agtacgggta tcaaccggca tttcggcttc
    22681 cagcacatca tcgcccagac gggtatacac aattcccaga tgcgccacca ttgtgttatc
    22741 gctggtggcg ttcagttcgt cgagcgttaa atggcgtttc cagatcatgc ttatgccccc
    22801 agcgttgagc cgccatcgac cacaatatcc tgtagggtaa tatggctggc gaggtcagag
    22861 gcgaggaaca aaatcgtgtt ggcgatctct tgtggacggg cgattttccc cagcggaatg
    22921 ccgagtttaa actgctcgcc aaagccgcga atacgctgtt cttcggcgtc atcgctcacc
    22981 cacagcgtgc gttgcatatc ggtgtcggtg gagccaggcg aaaccacatt acagcgcacg
    23041 ccgctacccg ccagttccag cccgacgctc aacgccaggc ttttcagcgc cgctttcgat
    23101 gcgccataag cactcatgcc aatacgcggc gtgtgtgcgg cgtcggacgc cacagtgaca
    23161 atcgccccgc cccgctgacg gcgaaactgg ttcatggttt gctgaaacag gttaaacgca
    23221 ccgccgacgt taaccgcaaa agtctgctgc cagtcctctt tactgagctg atcggtcgcg
    23281 cccatgcgca aaattcccgc cgcattgacc agcacgtcca gtcgctccgt ttcagctaac
    23341 agtcgctgac acacctgcgc cacctgcgca gcatcggcaa catccatcac ttcggtcgca
    23401 aagggatatt gctcctgagc gaacgcttga tcaaaaccgg taactttcgc tcccgcctca
    23461 acaaacgcca gcgccgtggc gtagccgata cctttacctg cgccggttac ccagacattt
    23521 ttaccgctga aatccattat ttcacctcgc gggagagtag cttccaccag gcgtcgatgg
    23581 tcgggttttt cgccagcatg acaaagtcga tatcaccatg cactttgcgc cagcgcgccg
    23641 ccagcgccat catgcgcacc gaatccagac cgtagtcgat caggttgtcg tcatcgaacg
    23701 gttcatcgga ctcgtccagc aacggcagga taacctcacg cagcgccgct ttgctggcgg
    23761 ggacaggtgc tggcagtaat tcttcagtca tcaccacccg gccagaccgc ccggccacat
    23821 atttcagcga catcaaatgc tcgtcacggc tgaaatcggc cagcgcatcc gccaccataa
    23881 acggtttaat atcgcgcata aatgcgtcgg ttgcggtggt catacagcca atgtgggcat
    23941 ataccccggt aataatcagc tggttacgtc cactctcttt gagcatttgc tccagcggag
    24001 aacgatgaaa cgcgctgtag cgccacttca ccagcaccgt gtcgtcggca tctggcgtca
    24061 ggcgatccac caccttttgc tgttccggcg aacgggtcag gcccggcccc cacatatcat
    24121 tcaacagcgc ccgatcttca tcgctctgct ctttcggctg ggcggtgtaa taaaccggga
    24181 tattgtgctg tttgcagtag tcgcgcagcg cagcaatatt cgcgatcacc tgctccatca
    24241 tcgggcagtt ctcgccccag aagctgacaa aatagtcctg catatcatgg attaacaacg
    24301 cggcacgttg cggttcaaag gcccagtcaa ctttattctg cggaatatcg tgagactccg
    24361 gcagtgcgta agcctgtaat tttggaatag ccatcgtgtt ctctccttca ggctgatgcg
    24421 cgtgacgcca gccactgacg taattgtttt ttatcgactt tcccgaccgc cgtcagcgga
    24481 agtgaatcca cacactccac gcgatccggt aatttaaatt cggcaatacc ctgttcacgc
    24541 aggaaacgac gcacctgcac cgcgcgcagc ggctctttta ccaccagata agcacagctt
    24601 ttttcgccca tcagctcatc ttccatgctc accagtgcgg catagatcac cgccggatgg
    24661 cgcagcagca ggttttcgat ctcttcggca gcgatcttct cgccgccacg gttgatctga
    24721 tctttctcgc gcccctgcac ggtgatgtaa ccctctggat caatagagat cagatcgccg
    24781 gaacagtaaa aaccgttggc atcaaaggca ctggcattgt gctgcggact tttgtaataa
    24841 ccgcggaagg tgtacggccc gcgcgtcatc aggcgtccaa cttccccttg cggcagtgga
    24901 tttccttcag catcagcaac ccatacttca tcgtccggac acattgggta accctgggta
    24961 tggataattt tctccgcgct atcatcaagt cgggtgtagt tcaccagccc ttccgccatg
    25021 ccaaacacct gctgcaacag gcagccaatc tcagcgggaa tacgcgccgc aagggtggct
    25081 gaaagacgtg cgccgccgac ctgtaacagt ttcagcgagg caagctgcgc ccggctttca
    25141 ccttcggtca gcgcctgcaa ccacaggctg actgccggtg gcaccagcgc ggtgacgttc
    25201 acctgatgtt tttcaatcaa tgggaagcaa agcgtggcgc tgggatcggc cgccagaaca
    25261 accgttcctc cggcaagaaa gacgcccagc gatcctggcg aactcatggc gtagttatga
    25321 gccgccggga tcgcgcacag gtagcgtgtc tgttgtgtga actgacaaat ctcgacgcta
    25381 cgacgcacgc tgtagtagta gtcgttatga gtgcgcggga tcagtttcgg tgtgccggtg
    25441 gtgccgccgg aaagctggaa ataggccact tcatcagcag gtgatggcgt ggcagtaaaa
    25501 tcctcagccg gatggttaat cgcatcctgc aagttatgct caccgctgtc gttgtgcagt
    25561 tgcaccacac gaatggagga atgttctgtg acgaaagtat tgaggaaatc atccccgcta
    25621 aacagcgcat gttggcgatc ggcaatcagc aatgcgggtt caatctggct ggcataggcg
    25681 ttcagttcac tacgctgatg gctgaacagc gccagcaccg gcgcaacgcc cagtttcagc
    25741 agcgcgaaaa aggtaatata caattcagcg acgttaccca gttgtaccag cgcggtttca
    25801 ccaggtttaa tgccctgacg gcgtaaacta cacgcgaggt tatccgccac ctgattcagc
    25861 tcccgataac tcaactgccg ctcgccgtcg ataaccgcga tgctatcact cgcagcgtgg
    25921 cgagtcagaa tgtcggtcag tggcaaatcc tgccagtagc ctttttcccg atagcgacgg
    25981 gcaaactctt ccggccagcg ggtgaatgga atgctcatcc tcgctcctta atgcaatcca
    26041 aaaacgttca acatggtaga aagtttgacg cctgtttcgc gccactcacc caacggtgac
    26101 gacgcaggca caatccccgc tccggcaaac agacgcacct gattttcccg cagcttcgcg
    26161 cagcggatgg tcaccaccca ttcgccgtta ccttcgctgt cacaccaacc cacaatgccg
    26221 ccaaacagtt cgcggtcgaa cggctccagt tcagcaataa cctgggtcgc ggcctgatgc
    26281 gggaaaccgc tcagcgcggg ggtcggatgc agcagacagg ccagagtcag tgcgttttct
    26341 tgcgaattcg ctttaccttc aaagggagtt gcgagatgcc acagcgtcgg cgtggtaatc
    26401 aattgtggag aggaaggaac gtgtaactca ctactgcgtt cgcgcagtac ctctttcatc
    26461 gcctgagtca ccagttcatg ttcatggcga tctttttctg acgccagtag acgattaccc
    26521 gcttcgcgat cgagcacttc atccggctga cgacgcgcgg aaccggctaa cggaatggag
    26581 ctaaaacgct cgccgtcttt acgtagcagc agttccgggc tggcccccag cagcacgcca
    26641 ccatcagcca gcgggacatg gaagttgtaa ctaaccgggt tttgcgcaat caaccgttcc
    26701 agcaatacgc cactatcaat ggcggcgtca gtggtgatat caatcaatcg tgacaacacc
    26761 actttgtcga cctgcggcgt ggcggtaagt gcggcggcgc gggcaaccat ctgttcaaac
    26821 gtggtttgtt caggaattgc ctggcgttcc accacgttca gcgactggct gcgggtaaaa
    26881 cggcgggctg aggtctgttt ttcctggcgg gagaacgact gccaggattc gggaatatac
    26941 agcgacgaag gctgacgtgg atcgaaggga atcgccccga ccatcaccgg atttttgatg
    27001 ccctgcgctt tggcatcggc aaacagcgcg gcgagttttt gctggaaggg actgtcgggc
    27061 gaatccccgt tcacagccgg ttcatcgaag cgggcgaaac atcctgacgt cgtaaaactg
    27121 cggtacggcg acataaagaa aaagcgattg ggcgcaagtg ttgccatggt ctgctgtact
    27181 tcctcagcca gtgacgtatc catatcatcc tccacaaaat gataaaggct ttaataatga
    27241 ttatcattta tattttcggt cgctaaccta aaagcaaacg cgcactatgt caactcttga
    27301 ggtaacgcaa tctgcaactc ggggttgcat ctggttgtgg ttacaatgaa aatgagaagc
    27361 attaacttca ttaataacag gaagctgact tgtgagactc gccccgctct accgcaacgc
    27421 ccttctatta acaggacttt tgctttcagg aatagccgca gttcaggctg ccgactggcc
    27481 gcgtcagatt actgacagcc ggggcactca taccctggaa agccagccgc agcgtattgt
    27541 ttccaccagc gtcaccctga ctggctcact gctggcgatt gatgctccgg tgatcgccag
    27601 cggcgcgacc acgccgaata accgcgtcgc ggatgaccag ggctttttac gccagtggag
    27661 caaggtggcg aacgaacgca aactgcaacg gctctatatc ggcgaaccga gcgccgaagc
    27721 cgttgccgcg caaatgccgg atctgatttt aattagcgca accggcgggg attcggcgct
    27781 ggcgctgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt acgacgacaa
    27841 aagctggcag tcgctgttaa cgcagcttgg cgaaattacc ggtcatgaga aacaagcggc
    27901 agagcggatt gcacagtttg ataagcaact ggcagcggcg aaagagcaaa tcaaattacc
    27961 gccgcagccg gtcactgcca ttgtctatac cgccgctgca cacagtgcca atctctggac
    28021 gccggaatca gcacaagggc agatgctgga acaactcggc tttacgctgg cgaagttgcc
    28081 cgcagggtta aacgccagcc aaagccaggg caaacgccat gacatcattc agcttggtgg
    28141 ggaaaatctg gctgctgggt taaacggtga atcgctattc ctgttcgccg gtgatcagaa
    28201 agacgccgat gctatttatg ctaatccgct gctcgcgcac ctgcctgcag tacaaaacaa
    28261 gcaggtttat gcgctgggaa ccgagacgtt ccgcctggat tactacagcg ccatgcaagt
    28321 gctggaacga cttaaggcgc tgttttaagc attaaccgtc agacgctgtc acctgcggcg
    28381 gcctctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg ccgataatca
    28441 acaaaccaaa accgctcgcg cttgcggaag caaccggtgt catcatcgct cccaaaccgc
    28501 ccagcagcgc cgcgcctatg gcatcgcccg tcacgttttg tgccgtccac aaaccgttaa
    28561 tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtgtattgc agcaacgagc
    28621 tgaccgcact caaccagccg aacagcgcca gacaaaccac gcctaaaatc cacatcggca
    28681 tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc atcaataacc
    28741 ctggtcgcgc gctatgcgcc agcttcccgc tggttaacgc gccaatagcc gcgccgagcg
    28801 ggatcgccgc atagagaaaa ccaatctgtg ccgccgacat ctgccagttg tcagccagcg
    28861 ccggatacag tacccgcacc gcgctcgcca tcgtcaataa accacccagc aacgcaatcc
    28921 ctcctaccag cgggctggcg agcagaaaac gaaatcctgc cagtaatgat ttcaacggat
    28981 gctcacgcgg ctgcggtggc ggtggcaacg ccggaaggct taacaacggt agcaaggtaa
    29041 taaacgtgcc cgccgccgcc agcccgtagt tccaggctac gccaccggtc gccagcaata
    29101 aaccgccaat catgggcgaa atcaccgacc ccagacgcac ggtcaacatg gtgatcgccc
    29161 cggcctgcat taagttttca cgccctacca gtgctggtgt cgccgccaat agcgccgtaa
    29221 cgccaagcga tgcgaaaaaa ccatcccata aaccaagtaa atagattgcc agcaatgacg
    29281 gctccggcag cagtgcatta aggcacagtc caatgaagcc aatgccacag gtgccgcgcg
    29341 ccagcaaaat cacttttttg cgctcatagc gatccgccag cacaccgccg accatcaggc
    29401 caacaaacat cgcgccgccg gtcagcgtca ccgaaagccc cacctgccag gtggaatgtg
    29461 tcatcatctg gatctgcacc ggcaccgcga cgccgagcaa acccagagac acaattgaga
    29521 tgaaacgagc gaggaatact gcgcgaaacg ccgggtgcgt tttcaacagg ctgaggttaa
    29581 gcagccagga ttgtttattc attacaatgc cttgccatca aatattttaa atacctattc
    29641 ttaggctgca catgctaaca tatccaaata agatcgataa cgataattaa tttcattatc
    29701 atggaagttc gtatgtctgg ttctgttgcc gtgacacgcg ccattgccgt gcccggattg
    29761 ctgttattac tgattatcgc gacggcatta agcctgctca ttggggcaaa atcactcccc
    29821 gcttccgtag tgctggaggc cttctccggc acctgccaga gcgccgactg caccatcgtg
    29881 ctcgacgcgc ggctgccgcg tacccttgcc ggtttactgg caggcggcgc gcttggcctt
    29941 gccggggcgt taatgcaaac cctcacccga aacccacttg ccgaccccgg cttgcttggc
    30001 gtgaacgccg gagccagctt tgccattgtg ctgggtgcgg cgctgtttgg ttactcttcc
    30061 gcgcaggaac aactggcgat ggccttcgcc ggggcgctgg tggcctcatt gattgttgcc
    30121 tttaccggca gtcagggcgg cgggcagtta agtccggtgc gtttaaccct ggcgggcgtg
    30181 gcgctggcgg cggtgctgga aggactgacc agcggcatcg ccctgcttaa tcctgacgtc
    30241 tacgatcagt tgcgtttctg gcaagccggt tcgctggata ttcgcaatct acatacctta
    30301 aaagtggtgc tgatcccggt gctgatcgcc ggagcaactg cgctattact gagtcgcgcg
    30361 ctgaacagtt tgagcctcgg cagcgacacc gcgacggcgc tgggcagtcg cgtggcgcgc
    30421 acacagttga ttggtctgct ggcgattacc gtgctttgtg gtagtgcgac ggcaatagtt
    30481 ggcccgattg cctttattgg cctgatgatg ccgcatatgg cgcgttggct ggtgggtgcc
    30541 gatcatcgct ggtcgctgcc cgtcacgcta cttgctaccc ctgccctgct gctgtttgcc
    30601 gatatcatcg ggcgggtgat tgtgcccggc gaactgcgcg tttctgtggt cagtgcgttt
    30661 attggtgcac cggtgctgat cttcctcgtg cgacgtaaaa cgcgaggtgg tgcatgattt
    30721 acgtctctcg ccgattactc atcacctgtt tgctgctggt ttccgcctgt gtggttgcag
    30781 gtatctgggg attacgcagc ggtgccgtca cgctggaaac ctcgcaggta ttcgccgcgc
    30841 tgatgggcga tgcgccgcgc agtatgacga tggtggtcac cgaatggcgt ttaccacgcg
    30901 tgctgatggc gctgttgatt ggcgcagcac tgggcgtcag tggcgcgatt tttcagtcgc
    30961 tgatgcgtaa cccgctcggc agccctgacg taatgggctt taacaccggg gcgtggagcg
    31021 gcgtgctggt ggcgatggtg ctgtttggtc aggacctgac ggctatcgcg ctgtcagcaa
    31081 tggtgggcgg cattgtcact tcgcttctgg tctggctgct cgcctggcgt aacggcatcg
    31141 acacctttcg gttgattatt atcggtatcg gtgttcgcgc catgctggtg gcctttaata
    31201 cctggctgtt gctgaaagcg tctttagaaa cggcgctaac agcgggtttg tggaatgccg
    31261 gatcgctcaa cggcctgacg tgggcaaaaa cctcgccttc cgcacccatc attatattga
    31321 tgctcattgc cgccaccttg ctggtgcggc gaatgcgctt gctggagatg ggcgatgata
    31381 ccgcctgcgc gctgggcgtc agcgtcgaac gttcgcgtct gttaatgatg ctggttgcag
    31441 tggtgcttac cgctgcggca acggcgcttg ccgggccgat ttcctttatt gctttagtcg
    31501 cgccgcacat tgcccgacgc attagcggca ccgctcgctg ggggctaacc caggcggcgc
    31561 tatgcggggc gctgttactg ctggcggccg atctctgcgc ccaacaactg tttatgccgt
    31621 atcaacttcc ggttggcgtc gttaccgtca gcctcggcgg tatttacctt atcgtcttgt
    31681 taattcagga gtctcgcaaa aaatgaccga atcagtagcc cgtttgcgcg gcgaacagtt
    31741 aaccctggga tatggcaaat ataccgttgc ggaaaatctg actgtagaaa tacctgatgg
    31801 tcacttcacg gcaattatcg ggccaaatgg ctgcggtaaa tccacgttgc tgcgtacctt
    31861 aagccgcctg atgacgcctg ctcacgggca tgtctggctg gatggcgagc acattcaaca
    31921 ttacgccagt aaagaagttg cacgccggat tggtctgttg gcgcaaaatg ctaccacgcc
    31981 gggtgatatc accgtgcagg agctggtggc gcgtgggcgt tatccgcatc aaccgctgtt
    32041 tacccgctgg cgcaaagaag atgaagaagc ggtaacgaaa gcgatgcagg ccacgggaat
    32101 aactcatctg gcagatcaaa gcgtggatac cctttctggc ggacaacgcc agcgagcgtg
    32161 gatcgcgatg gtgctggccc aggaaacggc aattatgctg ctcgacgaac ccacgacctg
    32221 gctggatatc agtcatcaga ttgatttgct ggagttgtta agcgaactga accgcgaaaa
    32281 aggctatacc ctggcggcgg tgctgcacga tcttaatcag gcctgtcgtt acgccagcca
    32341 tttgattgca ttgcgggaag ggaaaattgt tgctcaggga gcgccgaagg agattgtcac
    32401 tgctgaactg attgagcgca tttatggtct gcgctgcatg atcattgacg atccagttgc
    32461 cggaacgccg cttgtggtgc cgctcggacg aacggcacct tcaaccgcaa atagttaaac
    32521 taagtggtct gccatcatgg catcctgttt tctggatgcc atcgcatagc gcaacagcac
    32581 gccaccacaa gccaccatcc cgccgatcaa cgcggaaagg atcacaataa tcgccttacc
    32641 cggaccgtct tttttcactg gcaatgacgg gcttaactga tatttaaacg gcgtaaaatt
    32701 cacatcgttg acatgtgctt ttgttaattg ctcgacaaga tactgccgat tacgtaattc
    32761 accgttcagt tccgcaacgt cagtgaccgc tttttctatt tccagtttgc gttcaatacc
    32821 gtctgcaccg agagaaatag aaaaatcggg gtcatcttta acggcctgac cattactgta
    32881 aacgggcttt ttaattcctg ccgcgttggc aatgtcgagt gaataattga ggcgctgaat
    32941 gtttgcatca agttgatttt tcgttttaat gcgatcctga gccagttttt ctttttcaaa
    33001 ctgggttttg atctccagtt tattacggac gttttctagc gactctttca ccaccaacgt
    33061 agagatataa tcgatatacc cgctcaaaac ggtctgcgcc tcttcactgg ttggcgcggt
    33121 aaaacttagc gtccaggagg tatacagtga cggttcatct tttttcttac tggcattgtc
    33181 atcaaccgct ttcatttttt cgctcaatgc gacaattgcg cgatgcaaat ccagttcgtc
    33241 gattttcgcc tcttttaatt ggtccatcac ataaggtgat gaacgcaggt actcttccag
    33301 caagctaacc gactgaaact tcttgataaa caggttaaat gcttctgtac gatcaatttt
    33361 gatatccaga tccagcacac gaagcttagt gaatgatttc tccaactctt gccactgaac
    33421 aggttctgga ggcgtgacaa ccgccgcgct ggtccatttt tgcggcagga tgaaagagat
    33481 cagcaagcct gcgcaggcaa acgcaaaaac gaccgccatg accgtttttt tggcccgcca
    33541 taaaactgag attagattaa gtaaatcaat ttcattatta ctgggcgacg ccagaggata
    33601 atcgggaaaa tgagcgtcac ttccctgttt aatattcagt gatgacatgg ggtataacct
    33661 gaaaatgagt ccaatagggg gaaatctcca cggcgctcac tttaccagtt ggtgataatt
    33721 atccgaagct gaagtttgta aattccttcc actgaatgca acattcactc tattt
//
LOCUS       DAZFZC010000046        33727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFZC010000046 DAZFZC010000000
VERSION     DAZFZC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.046"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(164..748)
                     /locus_tag="ACHKXP_002580"
     CDS             complement(164..748)
                     /locus_tag="ACHKXP_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFO7647561.1"
                     /translation="MPANISGTPFNSFGISFIQKQSCWRKSDDILRCSMGQRTIKLST
                     NTLNNRILTSVARQSTKDINAWKRDEQTVYPSRVINQGIDKYCAENSRNISSEVRQRV
                     FKFIEKDYSLKLNIIAAQSSINHLIIGNGRFGDKINMLCKGVSREVKNQTMDVIANQL
                     ADQFFQKHISPDVDIKQLRDDIPRYIMAASVISA"
     gene            946..1584
                     /locus_tag="ACHKXP_002581"
     CDS             946..1584
                     /locus_tag="ACHKXP_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7647562.1"
                     /translation="MPVVLNISDGRALPESELEALRNIARSNQSDTLIVGSRSLRLHH
                     VSFMDAFSVEPIQGSLLDRLGGRCRCLAESLERQLNHGNTFLQAFSLYMEQVRLTPFW
                     LEGGRNAVQTRINSHAFTVNPGDFPCCEQHLSCPITLCIPKTGVFVKNALHSKVCSLY
                     DKDALSEAIRHNVFHPLSREAFSPEMIVGREECYFDLADQRFCIISGQDVRF"
     gene            1876..3241
                     /locus_tag="ACHKXP_002582"
                     /pseudo
     CDS             1876..3241
                     /locus_tag="ACHKXP_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EspK/GogB family type III secretion system
                     effector"
     gene            3299..3577
                     /locus_tag="ACHKXP_002583"
     CDS             3299..3577
                     /locus_tag="ACHKXP_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO7647563.1"
                     /translation="MPLSIGSCFSCHTGRRPEISDPIIDKPSTKNCELISTTCNVDGI
                     TVISRSSYGFDMKPPGAGDRVPRLKLSASEAQWMAAIIDAEGRNISNT"
     gene            complement(4312..4387)
                     /locus_tag="ACHKXP_002584"
     tRNA            complement(4312..4387)
                     /locus_tag="ACHKXP_002584"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4352..4354),aa:Phe,seq:gaa)
     gene            complement(4494..5069)
                     /locus_tag="ACHKXP_002585"
     CDS             complement(4494..5069)
                     /locus_tag="ACHKXP_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7647564.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(5106..6803)
                     /gene="dsbD"
                     /locus_tag="ACHKXP_002586"
     CDS             complement(5106..6803)
                     /gene="dsbD"
                     /locus_tag="ACHKXP_002586"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO7647565.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(6779..7117)
                     /gene="cutA"
                     /locus_tag="ACHKXP_002587"
     CDS             complement(6779..7117)
                     /gene="cutA"
                     /locus_tag="ACHKXP_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO7647566.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(7233..8534)
                     /gene="dcuA"
                     /locus_tag="ACHKXP_002588"
     CDS             complement(7233..8534)
                     /gene="dcuA"
                     /locus_tag="ACHKXP_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO7647567.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(8652..10088)
                     /gene="aspA"
                     /locus_tag="ACHKXP_002589"
     CDS             complement(8652..10088)
                     /gene="aspA"
                     /locus_tag="ACHKXP_002589"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO7647568.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            10425..10901
                     /locus_tag="ACHKXP_002590"
     CDS             10425..10901
                     /locus_tag="ACHKXP_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO7647569.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(10917..12173)
                     /gene="yjeH"
                     /locus_tag="ACHKXP_002591"
     CDS             complement(10917..12173)
                     /gene="yjeH"
                     /locus_tag="ACHKXP_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO7647570.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            12449..12742
                     /locus_tag="ACHKXP_002592"
     CDS             12449..12742
                     /locus_tag="ACHKXP_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO7647571.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            12786..14432
                     /gene="groL"
                     /locus_tag="ACHKXP_002593"
     CDS             12786..14432
                     /gene="groL"
                     /locus_tag="ACHKXP_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO7647572.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            14570..14923
                     /locus_tag="ACHKXP_002594"
     CDS             14570..14923
                     /locus_tag="ACHKXP_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO7647573.1"
                     /translation="MHVKYLAGIVGAALLMAGCGSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(15126..15995)
                     /locus_tag="ACHKXP_002595"
     CDS             complement(15126..15995)
                     /locus_tag="ACHKXP_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7647574.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(16390..17418)
                     /gene="epmB"
                     /locus_tag="ACHKXP_002596"
     CDS             complement(16390..17418)
                     /gene="epmB"
                     /locus_tag="ACHKXP_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7647575.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENKGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARVTDALVERFSHTTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17460..18026
                     /gene="efp"
                     /locus_tag="ACHKXP_002597"
     CDS             17460..18026
                     /gene="efp"
                     /locus_tag="ACHKXP_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7647576.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            18078..18203
                     /gene="ecnA"
                     /locus_tag="ACHKXP_002598"
     CDS             18078..18203
                     /gene="ecnA"
                     /locus_tag="ACHKXP_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7647577.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            18314..18460
                     /gene="ecnB"
                     /locus_tag="ACHKXP_002599"
     CDS             18314..18460
                     /gene="ecnB"
                     /locus_tag="ACHKXP_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7647578.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            18636..18953
                     /gene="sugE"
                     /locus_tag="ACHKXP_002600"
     CDS             18636..18953
                     /gene="sugE"
                     /locus_tag="ACHKXP_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7647579.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STQ"
     gene            complement(18947..19483)
                     /gene="blc"
                     /locus_tag="ACHKXP_002601"
     CDS             complement(18947..19483)
                     /gene="blc"
                     /locus_tag="ACHKXP_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7647580.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGSY"
     gene            complement(19572..20705)
                     /locus_tag="ACHKXP_002602"
     CDS             complement(19572..20705)
                     /locus_tag="ACHKXP_002602"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7647581.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQVAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(20768..21127)
                     /gene="frdD"
                     /locus_tag="ACHKXP_002603"
     CDS             complement(20768..21127)
                     /gene="frdD"
                     /locus_tag="ACHKXP_002603"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7647582.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(21138..21533)
                     /gene="frdC"
                     /locus_tag="ACHKXP_002604"
     CDS             complement(21138..21533)
                     /gene="frdC"
                     /locus_tag="ACHKXP_002604"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7647583.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(21544..22278)
                     /gene="frdB"
                     /locus_tag="ACHKXP_002605"
     CDS             complement(21544..22278)
                     /gene="frdB"
                     /locus_tag="ACHKXP_002605"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7647584.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(22271..24079)
                     /gene="frdA"
                     /locus_tag="ACHKXP_002606"
     CDS             complement(22271..24079)
                     /gene="frdA"
                     /locus_tag="ACHKXP_002606"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7647585.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            24404..25381
                     /gene="epmA"
                     /locus_tag="ACHKXP_002607"
     CDS             24404..25381
                     /gene="epmA"
                     /locus_tag="ACHKXP_002607"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO7647586.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            25600..27102
                     /gene="yjeM"
                     /locus_tag="ACHKXP_002608"
     CDS             25600..27102
                     /gene="yjeM"
                     /locus_tag="ACHKXP_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO7647587.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(27253..30576)
                     /gene="mscM"
                     /locus_tag="ACHKXP_002609"
     CDS             complement(27253..30576)
                     /gene="mscM"
                     /locus_tag="ACHKXP_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO7647588.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(30598..31566)
                     /gene="asd"
                     /locus_tag="ACHKXP_002610"
     CDS             complement(30598..31566)
                     /gene="asd"
                     /locus_tag="ACHKXP_002610"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO7647589.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(31663..32715)
                     /gene="rsgA"
                     /locus_tag="ACHKXP_002611"
     CDS             complement(31663..32715)
                     /gene="rsgA"
                     /locus_tag="ACHKXP_002611"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO7647590.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDISDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            32810..33355
                     /gene="orn"
                     /locus_tag="ACHKXP_002612"
     CDS             32810..33355
                     /gene="orn"
                     /locus_tag="ACHKXP_002612"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO7647591.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            33566..33641
                     /locus_tag="ACHKXP_002613"
     tRNA            33566..33641
                     /locus_tag="ACHKXP_002613"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33599..33601,aa:Gly,seq:gcc)
BASE COUNT         8314 a         8224 c         8675 g         8514 t
ORIGIN      
        1 gaatagagca agagggggtt gtttcgttaa ccagagcctg taatttttta tccataaatc
       61 taccgctatt tgatgttgga tttgacatat caacatcagg caacgacaca actctatgta
      121 caggctctta taggtatttt ttagcgttaa ggggtatctc tatttatgcc gatatgacgc
      181 tggcagccat aatatatctg gggatgtcat ctcgtagctg tttgatgtca acatctggac
      241 taatatgctt ttggaagaac tggtctgcga gttgattggc tattacatcc attgtctggt
      301 tctttacttc tctactcact cccttgcaaa gcatatttat tttatcaccg aaacggccat
      361 ttcctattat gagatggttg atagagcttt gcgctgctat gatattcaac ttgagactgt
      421 aatccttttc aatgaattta aaaactcttt gccgtacttc actggaaatg tttctgctat
      481 tttcagcaca atatttatca attccctggt tgatcacgcg agatgggtaa actgtctgtt
      541 catctctttt ccaggcatta atatctttgg tgctttgtcg ggcgacggag gtcaaaattc
      601 ggttgttcag cgtattagtg gataacttga tagtcctctg acccattgaa catcgcagga
      661 tatcatcaga tttcctccag caggactgtt tctggataaa gcttatacca aatgagttga
      721 agggtgttcc tgatatattt gctggcatag taattccttt tatttgtcta tatttacaat
      781 gtagcaagtt tataattatt ttagtatcgc aacgctttgg tgtaaataat gataagtcaa
      841 taattgatgg tatgttgcca aaatgttttg tctgttttat tttatgtgtg gatgtttatt
      901 tgttgttggc ctggataagc taaatacatt acagagggag ataatatgcc cgtcgtatta
      961 aatatctcag atggaagggc gctgcctgaa agtgaactgg aagctctgcg taatatagca
     1021 agaagcaatc agagtgatac tctgattgtg ggaagtcgta gtctgaggct gcaccatgtt
     1081 tcatttatgg atgcttttag tgtggagccg attcagggca gtcttcttga ccgtcttgga
     1141 gggaggtgtc gttgccttgc agagagtctg gagagacagc ttaatcatgg caatactttc
     1201 ctgcaggcgt tcagtttata tatggagcag gttcgtttaa ccccattctg gttggaaggg
     1261 ggcagaaatg cagtgcagac cagaattaat tctcatgcat ttaccgtcaa tcctggagat
     1321 tttccttgct gtgagcaaca tctgagttgc ccgataacat tatgtatacc aaaaacaggc
     1381 gtttttgtta aaaatgcact gcattcaaaa gtctgttctt tatacgataa ggatgccttg
     1441 tctgaggcta ttcgacacaa tgtttttcat cccctcagtc gtgaagcgtt ttctccagaa
     1501 atgatcgttg gcagagagga gtgttatttt gatctagctg atcagcgttt ctgcatcatt
     1561 tccgggcagg atgtacgttt ttgagtgggg gatattaata cagactgagt tgtactgact
     1621 gctgcttttt tattcgtctt cgattgacaa cattattttg ctggttgtta gaatccgggt
     1681 cagattgctg ggtttattct ggaatgaaca tgtagtagct gtacttgctt ttccagcgtg
     1741 aaatagcgtc tgtaatgatg ccttttcgcc atcgggaaac cctagataca ggctctctgt
     1801 atgaaatttg tcaggtaagc acctcggatg gggcttacct tatagtgatt tattttctga
     1861 aaatggaatt tattgatgct ttccacatca caattaaaat tgtcagggac tgcctgtcat
     1921 catattactg aaacacctgc aagctttaaa agtgaaatta cgtcccggca actggaagat
     1981 gcacgggaaa gtggttgtct gcatataaaa aattgtgaca tggagttctt gcctcattta
     2041 ccggaagaaa tcacagcgat aacaatagag aactgtaata agctgaccgg tcttacaggg
     2101 ctaccggtca atataaaaag cctgtccgtt attaattgtg agaatctgaa agttagtgaa
     2161 ctgccgccga cagtaagaga gctacatatt gaattgaata attcgtcttt tgtacatact
     2221 gttccagagg gcgttgagca tttgaaactt ggtcactgtc aggtatcggg tgtgcctgat
     2281 agtgtccggt ctcttgagtt gacgggtagg gctgcggaga cgcttaccgg cgtaccatca
     2341 gggttgtgtt ctctgagtat ccgggattat aacccggagt atcaggtcag aatcgataat
     2401 tttatatccc cttcactgca gacgttgtct ctgacgggtt gcagtaatat cgtgttgccg
     2461 gaaaaactcc caaaagggat ggtgtcagca accatttata cagagcagaa aacgacatgg
     2521 aacatcgttg ctgaaggatt tcccgaaggg atggatcttg atttacagaa tgtacagctt
     2581 tctccagatg tgattaaaga aaaaaatatt acttttcagg gctatgccat ggaggcggct
     2641 ttacattttc gtccagggga tattgtgtat gggctttcgg aacccagagg aaaagttgtt
     2701 aatacaataa aactggttaa tgattttttc taaaaaagat attttaatac agaatacgtt
     2761 aacaagtgct gtatgggatt ataaatctcc tcggaaatat aagaaagacg ctcttatcaa
     2821 gagagcatta agtgagtctg atagaggaat taactttaag cagtatttga agaaccatag
     2881 taaatataat gtcactatgg ccgatctttc tgtgtgtaat cgtgacaagt tatgggcaaa
     2941 aaccagtaag gctggcctgg agtttcagtt attagtaagg aacaaaatgg tgcatttttg
     3001 tgccgatggg cttgtcgata ctctaaaaaa tatagccaat aagggggatg cttatgggca
     3061 aagtattaca gccagtgagt tgcgatggat ttatcgtaat caggataatg agcagataat
     3121 gaaaaatata aatttttatc tacgtgggaa agaagttccg gcagagaaaa ttttaggcat
     3181 gccagaatgg aaagattatc atccgaaacg ttctggattg atatctgaat actctaaata
     3241 atgcacttct attatgacag tgcatatcag taaagtaaca tcggcaggag ataaaattat
     3301 gccattgtca atcggaagtt gtttttcatg tcacacagga cgtcgaccag aaatatctga
     3361 tccaataatt gataagccat caactaaaaa ttgtgaactg atatcaacaa catgtaatgt
     3421 ggatggtata acggttatat ccagaagctc ttatggtttt gatatgaagc cacctggtgc
     3481 gggggatcga gttccacggc taaaattatc ggcctcagaa gctcagtgga tggcagcaat
     3541 aatagatgca gaggggcgta atataagcaa tacctgacca tttactcagt gatgtgttat
     3601 ctgagggctg gcggagtttg tacacagatt gtgccgttgc ctgatgttga tatgttcagc
     3661 tcagcatcaa atgaaggcga ttatggatga aaaacggacc tccatggagg tccgttgata
     3721 tgagtctggt gcctgattca gaatcgaatt aaggattcat tgacacggca atgaaatcac
     3781 caaataacag taagttacct ttacacttcg attccaaatg taccactatt ttgtactgtt
     3841 caagtcgtat tacttctgat acatcatttt atgatctcgt ggaggacaga ggattctggt
     3901 aagggtaaga agcgagaagg aaagaagagt ctgttgcagg tggacggagt ggtgaagagc
     3961 cccatgtcaa tggcgtatca ggttttcccg cttgttattg aagattttcc caccttattc
     4021 tccgcgacaa aatcacgtta aaacctgcgg attaaggcga aatgataact ctcctgtttc
     4081 cccgtgtttt ctacttcatt atgaatgttc tgagaaaagg gaaccgtaga aagaccgtag
     4141 tagtagagta aggaaaaggc acacatcata ggatgttggg gtgtctaaaa gtcacgaaca
     4201 tcggcgaaaa tggtgagaaa tacaacaaac aaataacgtg ttgcaacgac cggaaatcta
     4261 ccgtagccag aaaagaaaaa agccccgctt tgcagcgagg ccttatgaat ttggtgcccg
     4321 gactcggaat cgaaccaagg acacggggat tttcaatccc ctgctctacc gactgagcta
     4381 tccgggcaac ggggcgcatt aaaccgtaat ctgcacatct cgtcaaccta atttcagtaa
     4441 aagcgattca actgcttaag attgcggcaa atcgcttctt ttctgtgttt ctgtcaggtc
     4501 aatgcaccac cctggcggca gcgggcgaag cgcaggatat cttccgccag ccgatgtgca
     4561 gtgtcgacat ccgcctggct acgattcacc agcattcggc ttaaacagcc ttccagcacc
     4621 agttccatct gctttgctac catcgccgga tcgtcaactt ccagtgtggt taacagttcg
     4681 tgggtgaaat cgtaggccgc gcttttttgc tgatcggcca gttgatgaat agggtggcca
     4741 ggatcgggat aaaacgtaca ggcagcgata aatagacagc ccggatagcg gttgttttta
     4801 acgcactccg ataacgcctg ataacgtgcc agcagctttt gttcggcggt ttgcgtttcg
     4861 tccagcatca gctgacgacg ccagacatct atctgttggc taagataacg cagcgcatcg
     4921 tagaggattg cctctttgtc tggccagaag cggcgtagct cgtccagtgg ataatccaca
     4981 cgttcagcaa ccatctccag cgtggtgttg gcaatccctt gtaattctaa taatttcagg
     5041 gcttctccca gtacatcttc acgttgcacg ctattttcct ccgtctttcc cactgcaagt
     5101 gtcgttcacg gttggcgatc gcgcaaatgt gcgctgaagg tttcagcatc cataaagccc
     5161 gtgacgcgtg cttgtggatg ctcctggcct tgtccgtcaa aaaagagaat tgtcggtagg
     5221 ccaaggacat taagatgctt taacagcgcc acatcttgtg cgtcgttggc cgtgacgttg
     5281 gcctgaagta agaccgtgtc tgctaacgct ttttgcacct gcgggtcgct gaaggtgtat
     5341 ttctcaaact ctttacaggc gacgcaccag tcggcataaa gatctaacat caccggtttg
     5401 cctttggctt caacgagcgc ctgatttaac tcatctaccg ttttgatttg tgtaaagttg
     5461 agatgcgtct gagtttgcgc ggtatgcgtc gcaccaaatg cccaatcctg aagtgggcgc
     5521 acgctaacca atgccgctgc cagcaggatt atttgcacca cgcgcatcca gccgcgttta
     5581 gcctgtaggc tggtgataaa ggcccagcca aagaatgcga cacccagcgc cgaccacaag
     5641 cgtaatcccc atacatcacc aatcactcgc tccagcagga agaccggcag tgcgaggatc
     5701 acaaaaccaa acgcggtttt gacttgttcc atccacgggc cgcttttcgg cagcaagcgg
     5761 ttaccaaaga cggtaattag catcagcggc aggcccatgc ccaacgcata gagataaagc
     5821 gtgccgccgc ccagccacat gttcccgctt tgggcgatat acagcagaat cgcgctaagc
     5881 ggtgcggtgg tgcatggtga acagatcagt ccggcaatcg cccccataac aaacacaccg
     5941 ccaggtgagc cgccctgttg gcgattgctc atcaacgtga gacgtgtttg cagcgaagag
     6001 gggagttgta gagtaaacaa gccaaacatt gacatcgcca gcaaggtaaa gacgatggcg
     6061 aggccaatga gcacgtatgg gtgctgtagc gccgcctgga actgtaaccc tgcggcggca
     6121 actaccagac ccagcgccgt gtaagtcagt gccatcccct gcacataaat aaaggtcagc
     6181 aacaatgctc tggcagtgga gagccgctgt ttaccaccca gcacgatgcc agaaatcagt
     6241 gggtacattg gcagcacgca tggcgtaaag gcgataccaa taccgatcaa caacgcccag
     6301 agcgcggaaa agggcaattg cgcggtgtgc tgctcttgct gcggaacaga cacaggctgt
     6361 gacgctgcgt tgttggctac cacttcgctt aacggaacgg ttttggtttc tggcggataa
     6421 cagaaaccgg catcagcaca gccctggtag gtgacagtta acgtcgctcc cgcactcgcc
     6481 tggttgatgg ttacgggaag cgtcagccga tcgcggtaaa tctcgctttt gccgtaaaac
     6541 tcatcttcat gccagacgcc ttgcggcagc tgcacgtcgg caattttcgc gtgttccggc
     6601 gtaatgcgga tctgtttacg gtagaggtag taaccgtctt tgatctgcca ggtcagatta
     6661 aggtcatgtt ggttttgctg aaaatcaaaa gcaaaggctt gatccgctgg gacaaattgt
     6721 gaacgtcccg gcgcgtcgaa taatccggca aaaacggaag tgctgcaaag tagcaggatc
     6781 agcgtaaaga tgcgttgagc catgagaggt aatctgtgtc tccgtgtgta acaggtaaaa
     6841 ccagaagttc cggggtttga tatggatgat gagacttcag gcattccagc agtgcctgct
     6901 ggtgagatac ggtagttttt aaaatcatct gcacttcgta ttcttgctcc agcttacctt
     6961 cccagtaata gagagaggta gcgccgggga tcaaggtcgc gcaggccgcc agtttttccg
     7021 ccagcacttt ggcggctaaa tcctgggctg tcgcttcatc cggtgcggta catagcacca
     7081 cgacagacgc ggtattcgaa cttttttcat caagcataaa cacctcgcaa gaacagatgg
     7141 aaccgcaaga gagaggtcac tatacaacgg acgggggaag gatgttagtc ggcgggaaag
     7201 aaagcggggc gtgaacgccc cgcgattggt cattacagca tgaagctacc cagcacgaag
     7261 ccgaagcaaa cggccagggc aacacccaga gtacccggga tgaagaacgg atggttgaag
     7321 acgaatttac cgatacgggt agtacccgtg tcatccatct gtaccgcagc aaccagcgtc
     7381 gggtaggtcg gcagaatgaa cagaccagac accgcagcga aagaagcaac agcggtcagc
     7441 ggagaaacgt tcagtgccag agccatcggc atcagtgctt ttgcggttgc agcctgagag
     7501 tacagcagag cagaagcaaa gaagaagatg acggccagca gccacggatg accctgaatc
     7561 acttcaccag cggtatcttt gatccagtcg atgttgttgg aaacgaaagt atcgcccagc
     7621 cacgcaacac ccaggataca aatacaggcg ctcatacctg ctttgaaggt gctggagttg
     7681 aggatgttgt cggtatcgac tttacagata acggtggtca gagttgcaac gctgagcatg
     7741 atgatcagga ttgcgttggt ggtgttcatc agcggttttt caaccagacc catgcttggg
     7801 ctgttgatga ttgcatagat aaccacgcca actacgccca gcaggaacag ccagacggac
     7861 gtttttgcac cggatttgat ttcaatctgc ttttcaccgc gcagttcaac caggccctct
     7921 tccagacgct tgcgataaat cggatcgtca gagagtttgg agttgaacag catagtgacc
     7981 agaaaggaca tcaccagaac cgccagcagg gtggacggga tgaccacgga gagcagatgg
     8041 aggtagctga tgccatgacc ttccatcacg gaagacatgt aaaccactgc cgctgagatt
     8101 ggcgatgcgg tgatcgcaat ctgcgcggat accactgcag tagacagcgg acggcaaggt
     8161 ttgacgcctt gttccttcgc aacttcagcg ataactggca gtgtcgccag agagatgttg
     8221 ccagtaccag caaagatagt caggaaatag gtcacgatcg gtgcgaggat cgtgatgtat
     8281 ttcgggttac ggcgcagcag cttttctgtc tgatgaacca gatagtccag accgccagca
     8341 acctgcatgg cagaaatagc ggcgataacc gccatgataa tggaaatgac atcgaacggg
     8401 atgttaccgg gtttaacgcc aatagcggca agaaccagca cccccaatcc gcctgcaaaa
     8461 ccaataccta ttccccccaa tctggcgccc aagaagatcg ccagcaaaac tatgatgagt
     8521 tctacaacta gcatattagc cttccttgtt ttttaacaag ttgatattag gttgttattt
     8581 ttaagttact gctcacaaga aaaaaggcac gtcatctgac gtgccttctt tatttgtact
     8641 accctgtacg attactgttc gctttcatca gtatagcgtt ttgctttgta agccgggtgc
     8701 atcagattct gtacggagaa aatatcgtca agttccgctt cagtcaacag accgcgttcc
     8761 agaacgactt cacgtacact cttaccggtt tcggcacaga ttttacccac gatgtcaccg
     8821 ttgtggtgac cgatgaacgg gttcaggtaa gtaacgatac cgatagagtt gtaaacgtaa
     8881 ccttcgcaca cttctttgtt agcagtgatg ccgttaatgc atttttccag caggttgtag
     8941 caagcgttgg tcagaatgtg aacggattcg aacatagcct ggccaatgac cggctccata
     9001 acgttcaact gcagctgacc tgcttctgct gccatggtaa cagtggtgtc gttaccgatg
     9061 actttgaagc atacctggtt aaccacttcc ggaacaaccg ggtttacttt agctggcatg
     9121 atggaagagc ccgcctgcag ttccggcagg ttgatctcgt tcaggccggc acgtgggcca
     9181 gaagagagca agcgcaggtc gttacagatt ttggacatct tcacagccag gcgtttcagc
     9241 gcgccgtgaa ccataacata agcgccgcag tcagaggtcg cttcgatcag gtcttcagcc
     9301 ggtacgcatg ggaagccagt gacttcagcc agttttttca ctgccagcgg agagtactct
     9361 ttcggcgtgt tcagaccagt accgattgct gttgcgccaa ggttaacttc cagcagcagt
     9421 tcagcggtac gttggatgtt tttcacttct tctttcagca ggatgctgaa agcgcggaat
     9481 tcctgaccga gggtcatcgg tactgcgtcc tgcagctggg tacgacccat tttcaggatg
     9541 tcctggaatt cgacagcttt acgttcaaag ccttcacgca gttggttaat cgcatctacc
     9601 agcttaatca gggaagagta aactgcgata cggaaaccgg tcgggtaggc gtcgttagtg
     9661 gactgacatt tgttaacatg gtcgttcggg ttcaggtact gatattcacc tttctggtga
     9721 cccatcagtt ccagaccgat attggccagc acttcgttgg tgttcatgtt tacggaagta
     9781 cctgcgccgc cctggtagac gtctaccggg aactgatcca tgcattttcc gttgttcagg
     9841 acttcatcac atgcggcaat gatggcattc gctacacttt taggaatggt ttgcagctct
     9901 ttgtttgcca tagctgcggc ttttttaacc attaccatac cgcgaacaaa ttcaggaata
     9961 tcactgattt tgttgttgct gatatagaag ttttcaatcg ctctcagagt gtgaacacca
    10021 tagtaggcat cagctggaac ttccctggta cccaacagat cttcttcgat acgaatgttg
    10081 tttgacatgt gaaccttctt tttcaagctg ccaatgattt gctttaaaca cacagaatat
    10141 atgtggtttc gaatgttttt cgaccgacga ttatcccctg catcgaccga atacccgaga
    10201 tcatatgctg cttgaggatt tctaccgtaa tctggatcac tttaagtgtc ggtttttacc
    10261 ccttaattat taatttgtga aatagatcac cgctttggga ttactaccaa aaatagttgc
    10321 gcaaacatct tgaaattttg ctaatgacca caatataagc tgaacgcgat tcgcaaccca
    10381 ttcaggtagc cgggttttac cggctgctat tacaggagaa acctttgcgc tggttacctt
    10441 ttattgccat tttcctttat gtctatattg agatttcaat ctttattcag gttgcccatg
    10501 tattgggggt attgctgacc ctcgtgctgg ttatattcac gtcagttatc gggatgtcac
    10561 tggtacgtaa ccagggcttt aagaatttcg tgctgatgca gcaaaaaatg gcggcgggtg
    10621 aaaacccagc ggcggagatg attaaaagtg tttcgctgat cattgccggt ttgctgcttt
    10681 tattaccggg ctttttcacc gacttcctcg gtcttctgct tttattaccg ccggtgcaaa
    10741 agcatctgac agtgaagttg atgccgcatt tgcgcttttc tcgtatgcca ggcggcggtt
    10801 ttagcgccgg gaccggtggc ggtaatactt ttgatggtga gtaccagcga aaggatgatg
    10861 agcgcgaccg ccttgatcat aaagacgatc gccaggatta atgtcgaaac gccggattat
    10921 gtggttatgc cattttccgg cgtttttcgt tttggcagaa acagccataa ccccgccagc
    10981 atgatcagcg catagagact tttccagccg accattgcca gtaacagaac gcataacagc
    11041 ccgccaacca ccgccagtag tcgataacgt ccttgcaata atttacagcc tgccagcatg
    11101 cataacagat aaatcataat aaagatgcca ttggcataaa taataagagc gtccagattg
    11161 atctctaaag catgaatcac caacgtgctc accacgcagc agccgagcac cgcattgagg
    11221 gcattattcg ggatatggcg agaagagagg cgtgccaggt agtggtcagg attatgttgc
    11281 gcctgcgacc agaccaggcg ggcgaagctc tgtatataaa tgttgagact ggcaaagcag
    11341 gccagatagc caattacgca ggcaatccat aacgctccta caccgaacag ttgcactaca
    11401 atttttggaa gcgatgctgc cgcggccatt tgttcaccat aggcgtcgaa gtgtaagaca
    11461 actaccgtac agccccagta gactaatcct gccagcagca gaccaatcat caacgcacga
    11521 ggaaaatcac gctctggatt tttaaattcc gaggcaagat gggcaaatgc ttccagaccg
    11581 acaaaacacc agaacatcac tgataacgca gcaaataacc cggtaagttc gatatttcct
    11641 ggcgcaggga aggggatact cgcaggtttg atatcgcccg cccaccagat agcgacaatc
    11701 agcgcgacga taagtccggc aataacggtt tgtagatttg cactggaact ggcaccgcga
    11761 gtaccgatat accacaccaa cgccagcgta ccgagttctg ccaacaacag ttgccagcta
    11821 tgccagccaa acattgcctg gccgaatccg gcagcaattt gtagcgcggc aggcaaaccc
    11881 acgggaatga ccgataaaaa caaccagccg gtgactcgct caagccgcga accaaaagcc
    11941 ataccgacga agtgcgcgac accgcctgcg ctgggatagt ggcgacccag aatcgcaaac
    12001 acaatcgcaa tcgggaacac taagataatc aaaacgggcc acgcccacag gctgttattg
    12061 cctgctacca gcgcagctaa cgcaggaacg gcaaacacgc cagtgcctaa taatgacgtc
    12121 gatagcaggc caatgccctg ggccagcccc agttcttgtt tgagtccact catgggttga
    12181 tgtccgattg cgcccaaatt ttgggcaact gcgtagattt tcgatggtag cacaatcaga
    12241 ttcgcttatg acggtgatga agaaattgcg atgaaatgtg aggtgaatca gggttttcac
    12301 ccgattttgt gctgatcaga attttttttc tttttccccc ttgaaggggc gaagcctcat
    12361 ccccatttct ctggtcacca gccgggaaac cacgtaagct ccggcgtcac ccataacaga
    12421 tacggacttt ctcaaaggag agttatcaat gaatattcgt ccattgcatg atcgcgtgat
    12481 cgtcaagcgt aaagaagttg aaactaaatc tgctggcggc atcgttctga ccggctctgc
    12541 agcggctaaa tccacccgtg gcgaagtgct ggctgtcggc aatggccgta tccttgaaaa
    12601 tggcgaagtg aagccgctgg atgtgaaagt tggcgacatc gttattttca acgatggcta
    12661 cggtgtgaaa tctgagaaga tcgacaatga agaagtgttg atcatgtccg aaagcgacat
    12721 tctggcaatt gttgaagcgt aatccgcgca cgacactgaa catacgaatt taaggaataa
    12781 agataatggc agctaaagac gtaaaattcg gtaacgacgc tcgtgtgaaa atgctgcgcg
    12841 gcgtaaacgt actggcagat gcagtgaaag ttaccctcgg tccgaaaggc cgtaacgtag
    12901 ttctggataa atctttcggt gcaccgacca tcaccaaaga tggtgtttcc gttgctcgtg
    12961 aaatcgaact ggaagacaag ttcgaaaata tgggtgcgca gatggtgaaa gaagttgcct
    13021 ccaaagcgaa cgacgctgca ggcgacggta ccaccactgc aaccgtactg gctcaggcta
    13081 tcatcactga aggtctgaaa gctgttgctg cgggcatgaa cccgatggac ctgaaacgtg
    13141 gtatcgacaa agcggttacc gctgcagttg aagaactgaa agcgctgtcc gtaccgtgct
    13201 ctgattctaa agcgattgct caggttggta ccatctccgc taactccgac gaaaccgtag
    13261 gtaaactgat cgcagaagcg atggacaaag tcggtaaaga aggcgttatc accgttgaag
    13321 acggtaccgg tctgcaggac gaactggacg tggttgaagg tatgcagttc gaccgtggct
    13381 acctgtctcc ttacttcatc aacaagccgg aaactggcgc agtagaactg gaaagcccgt
    13441 tcatcctgct ggctgacaag aaaatctcca acatccgcga aatgctgccg gttctggaag
    13501 ctgttgccaa agcaggcaaa ccgctgctga tcatcgctga agatgttgaa ggcgaagcgc
    13561 tggcaactct ggttgttaac accatgcgtg gcatcgtgaa agttgctgca gttaaagcac
    13621 cgggcttcgg cgatcgtcgt aaagctatgc tgcaggatat cgcaaccctg actggcggta
    13681 ccgtgatctc tgaagagatc ggtatggagc tggaaaaagc aaccctggaa gacctgggtc
    13741 aggctaaacg tgttgtgatc aacaaagaca ccaccactat catcgatggc gtgggtgaag
    13801 aagctgcaat ccagggccgt gttgctcaga tccgtcagca gattgaagaa gcaacttctg
    13861 actacgaccg tgaaaaactg caggaacgcg tagcgaaact ggcaggcggc gttgcagtta
    13921 tcaaagtagg tgctgctacc gaagttgaaa tgaaagagaa aaaagcacgc gttgaagatg
    13981 ccctgcacgc gacccgtgca gcggtagaag agggcgtggt tgctggtggt ggtgttgcgc
    14041 tgatccgcgt agcgtctaaa ctggctgacc tgcgtggtca gaacgaagac cagaacgtgg
    14101 gtatcaaagt tgcactgcgt gcaatggaag ctccgctgcg tcagatcgta ttgaactgcg
    14161 gcgaagaacc gtctgttgtt gctaacaccg ttaaaggcgg cgacggcaac tacggttaca
    14221 acgcagcaac cgaagaatac ggcaacatga tcgacatggg tatcctggat ccaaccaaag
    14281 taactcgttc tgctctgcag tacgcagctt ctgtggctgg cctgatgatc accaccgagt
    14341 gcatggttac cgacctgccg aaaaacgatg cagctgactt aggcgctgct ggcggtatgg
    14401 gcggcatggg tggcatgggc ggcatgatgt aattgccctg cacctcgcag aaataaacaa
    14461 acccccgggc agaaatgtct gggggttttt cttttggtca tctttctagt ataagattca
    14521 gacacggacg acgcgagtgg cgtccagctc attgattatg gggaataaca tgcacgtaaa
    14581 atacttagca gggattgtcg gtgccgcgct actgatggcg ggttgtggct ccagcaacga
    14641 attgagtgct gccggtcaga gtgtacgcat tgtggacgag cagccaggcg cagagtgcca
    14701 gctgattggt actgcgacag gtaagcaaag caactggctt tccgggcaac acggagaaga
    14761 gggcggttct atgcgcggcg cagcaaacga tctgcgcaac caggcggctg caatgggcgg
    14821 taacgtgatt tatggcatca gcagcccgtc gcagggaatg ttgtccagtt ttgtcccgac
    14881 ggatagccag attatcggtc aggtttataa gtgcccgaac tgatgtgatc cagaggctgg
    14941 gtgacaaacg tattattgcc tgatgcgctt cgcttatcag gcctacagca ggcattgcaa
    15001 ctgattgaat gcttgagaat ttgtaggccg gataaggcgt ttacgccgca tccggcatga
    15061 ccaacgatca cgttatcagt catttcaggc tggatggctc cagcctcgca ttagcaaaga
    15121 tgagattatt tcgcctgtgg tgcagttttg ctggtggatt gaacacgtaa gtgatgcagc
    15181 gcgcgtaagc attgatcctg ggttagcggt tgggcgttat tcgccgctac ggtgcgaaca
    15241 tagacctgac atggcacacc cgcgagtttt ttcagtcgtg ggctgaaatc aagcattcgg
    15301 cgagtgatcg cctctatttc tttacccggc gttgcttcac gcgttttcac cactgcgccg
    15361 ctaaattgtt ctttgccact ttcatcttta aagcggtaaa gcaccgccag ataatggtcg
    15421 aacaagttat gtttccattc cggttgggaa taggtgtcat attccagatt gccattttcc
    15481 tggcagtcaa cgtcatataa cggcaggaaa tctaacagtg aggtaatacg taatgccagt
    15541 tcacgcattt ccgcattatt aattgcatga atgattgctc gcgccagcat ttcaatctgc
    15601 aactcattga cgactaactc acatttgcta ccgtcatgta gcgttaatac gaaggtaaga
    15661 ttttcgcctt gattatcggt aagttccagc gtgttaaccc gacgattaat atcggcattt
    15721 ttaagctcat caaccagaat ttctgggatg ttttctgcca ttttcttaat gactttatcg
    15781 cgagcttgct catactgaag gcgtgcagca gcatccagtt tatgcttttg atgcagacga
    15841 ctttccagcg caatcagcag gtcgcgcagt tccatgacag acataaagaa caacgattct
    15901 ttattgcgag gttctttaat tttcagcgcc agtgcaatga aattattact cttgcgaata
    15961 acaccggtat taacaccctt gatgcttatg gccatgtagg ttctccctaa ccatttctca
    16021 attaaataat taattttaat ttataagcca gataaatggg cttggtagta atagttgtta
    16081 aaataacata aatagccgtg ctcactctat ataaacagaa aagaaaggtt aattattggt
    16141 gttagctata taaaaagtaa caacagcaat gcatatgaat aattaaaggt tatgcaacgt
    16201 gcaaagatta aatttctcag tttaatgaca ggttgctgta cttgcgtttg ttgctcttgc
    16261 cggatgcggc gtaaacgcct tatccggcct acagttcatt gcaaatccat tcaatttcaa
    16321 tgcacgatgt aggcctgata agcgtagcgc atcaggcaat tttaatgttt aacttccctg
    16381 tttaatcagt tactgctggc gtagctggag atccaacggc gttttgctgg gttcgccacc
    16441 aatttcacgc gccagtttcg gcaccagata tcccgacacc agtgtcagca actcacgcat
    16501 aatctgccgt gcttcgtcat cactcaccat aaaatgcgcc gcgccctgta ctttatcgag
    16561 cacatgcagg taatacggca tcacgccggc atcgaacaac gcattactca ggtttgccag
    16621 tgtttgcgcg ttatcgttca cgccacgtaa cagaacactc tggttcagca aagtaacgcc
    16681 tacccggcgc aacttagcca ttgcctgacg gaatgtttca tctacctcat tggcatggtt
    16741 gatgtgattc accagcaaga tttgcagcgt agtatgtgaa aagcgttcaa ccagcgcatc
    16801 agtgacgcgt gccgggatca caatcggcag acggctgtga atccgcagac gtttgatatg
    16861 cgggatggct tccagttgcg tgagcaacca gtccagctcg tgatctttcg ccatcagcgg
    16921 atcgccgccg gagaaaatca tctcgtccag ttccggatgt gcagcaacat actcaagtgc
    16981 agtttgccag ttacgcttgt tgcccttatt ttcggcatag gggaaatggc gacggaagca
    17041 ataacggcaa tttaccgcgc agccgccttt gaccagcaaa agcgcccggt tgtggtattt
    17101 atgcaacaaa ccaggcacta cgctgtgctg ttcttccagc gggtcggtgg agaatccggg
    17161 cgcgacgaca aactcatctt gcgaggtaag tacctgacgc aaaagaggat cgtccggatt
    17221 gcctttctcc atacgatcga taaatgagcg gggcacacgc agggcaaaca gctttttggc
    17281 gctgcgtccg gccaacagtt tttcgtccgc gtctatattc aaaagacgca gaagttcatc
    17341 aggatcggtc acaacatcgg caagttgcgt taaccaatct tctctggatg gggtatttag
    17401 ggttacaata tgcgccattt tgtggcttag ctaccaatta acaaatttca gagggcctta
    17461 tggcaacgta ctatagcaac gattttcgtg ctggtcttaa aatcatgtta gacggcgaac
    17521 cttacgcggt tgaagcgagt gaattcgtaa aaccgggtaa aggccaggca tttgctcgcg
    17581 ttaaactgcg tcgtctgctg accggtactc gcgtagaaaa aaccttcaaa tctactgatt
    17641 ccgctgaagg cgctgatgtt gtcgatatga acctgactta cctgtacaac gacggtgagt
    17701 tctggcactt catgaacaac gaaactttcg agcagctgtc tgctgatgca aaagcaattg
    17761 gcgataacgc taaatggttg ctggatcagg cagagtgtat cgtaactctg tggaatggtc
    17821 agccgatctc cgttactccg ccgaacttcg ttgaactgga aatcgttgat accgatccgg
    17881 gcctgaaagg tgataccgca ggtactggcg gcaaaccggc taccctgtct actggcgctg
    17941 tggttaaagt tccgctgttt gtacaaatcg gcgaagtcat caaagtggat acccgctctg
    18001 gtgaatacgt ctctcgcgtt aagtaatgcg gttgtggtgc ggcctgcagg ctgcaccatc
    18061 acttattcag gtcagagatg atgaaacgcc ttatcgttct tgttttgctt gccagcacgc
    18121 tgctcacggg ctgtaacacc gctcgcggtt tcggcgaaga catcaaacat ctcggcaact
    18181 ccatctcccg cgctgccagc taatttttct tctcttccga aaaatcatca gatttccatc
    18241 atttttggtg atgttgtcta ttattaattt gctataggca aacataaata acattaccta
    18301 aaaggaagac gttatggtga agaagacaat tgcagcgatc ttttctgttc tggtgctttc
    18361 aacagtatta actgcctgca acaccacgcg tggcgttggt gaagacattt ctgatggcgg
    18421 taacgcgatt tctggcgcag caacgaaagc gcagcaataa gcaataacgg tacgacagct
    18481 gtgtcgtgcc gtttgttttt tctgtgatag tcataaaggt aatagttgaa attcccctgc
    18541 cacctggcaa aatatccgtt caaccatcag ctttgcagga cgacctgcaa acgcctcttt
    18601 tcaccgggga cggccccaat tctccggagc ctgatatgtc ctggattatc ttagttattg
    18661 ctggtctgct ggaagtggta tgggccgttg gcctgaaata tacccacggc tttagtcgtt
    18721 tgacgccgag tgttattact gtgacggcga tgattgtcag tatggcgcta cttgcctggg
    18781 cgatgaaatc gttaccagta gggacggctt atgccgtgtg gacgggtatt ggcgcagtcg
    18841 gcgcggccat caccggcatt gtactgctcg gtgagtccgc taacccgatg cgcctggcga
    18901 gtctggcgtt aatcgtattg gggattattg gtctgaaact cagcactcaa taactaccgg
    18961 gctgctgtac ccaaataaac ttactgacat caaacccttc ccgggtcgcg actgccagca
    19021 tctcctgttt cacttcgtca gaaatggttg gcgtgcggga gagtatccac aggtagtcgc
    19081 ggtccgggcc gcaaaccagc gcatggcggt attcccgatc aagtgcaatg acgttataac
    19141 cgccatagaa aggaccaaag aatgacactt tcagcgcagc gcgagttggt gcgccggtaa
    19201 agtacgcttt cccttcactc tgttgccaca ttcctctgtc agggttatag cctttattaa
    19261 tgacattcag gccgccgtca tcacgcaggc tgtatgttgc ggtgactttt tccagtccac
    19321 gttcaaagcg gtgatcaaaa cgggcaatct cataccaggt accgagataa cgtttggcat
    19381 cgaaattatt cactacggtc acgccacgcg gcggcgtagg agaactgcag gcaacgacca
    19441 gaaatgcagc tgtcgctgcg gcaacgagag ggagcaggcg cataaatgtt tccttactgg
    19501 tttttttcta agtgtagatg acagcaagga aaagcgcaga aaaggtccga aaattcggac
    19561 ccgatgaaat tttactgcag agcgttgaga atctgccagg cggcggcgac tctcgctgga
    19621 ttgggatagt ttttgttagc cagcatcacg atacccagct ctttttctgg aataaacgcg
    19681 acatagctac caaatccgcc ggtcgcccct gttttatgta cccatgatgc gcgtactgca
    19741 ggagttgggg gcgtaatcgc ttttacgggg cgtgctgcca gtgcaatttt attgtcactg
    19801 ccgttaacga tgatgtcagg atttaccggc cagtccagca tttcccagcc cagaccctga
    19861 tacatatcgc cggtttgcca gtagcgagat tgtgccactt gtatcccttg ttgaagcgtt
    19921 ttctcattga tatcaagggg ttttaaattg atttgcaccc agcgggccat atcttcaatg
    19981 gtcgacttca caccataagc ttcagcatct aacgcccctg gcgaaacatg cactgcctta
    20041 ccttcgcgat atccccaggc gtaattcttt tcttctgcgg gcggtacatt aatccatgta
    20101 tggttgagtt tgagtggctg gaagacacga gtttgcatcg cctgctcaaa actcaaacca
    20161 gacggcttca cagccagtgc gccgaacaaa ccgatactgg agttggcata cagacgttgt
    20221 gttcctggag cccatgcagg ctgccagttt tgatagaagc gcagcaagtc gcttgaggat
    20281 ttcacttcat ccggcacctg caatggcagg ccgccagcag tgtaggttgc gagatgtaat
    20341 agtgtgatcc cattccactg tttagcggta agttcaggcc agtattttgt tgcgggatcg
    20401 cttaacttga tttcccctcg agcaatagcg tcgccaccaa gcacgcccgt aaatgttttg
    20461 ctgaccgaac ctaactcaaa caacgtttgc tgtgtgacgg gctgcttttt ggcgatgtcc
    20521 gcatagcccc aggtaaagta ataaggttta ccctgataaa ttaccgccac cgccataccg
    20581 gggatctttt gttgctctat aagcggggta attgtgcgat gcacaatatc gttgattttt
    20641 tggggggcag caaatgtgga gcaagaggcg gtaattaata aggcgcagag cgtcgttttg
    20701 aacatagggt ctggtttcca tacaaaacgg cccgccatag gcgggccgga tttacattgg
    20761 cgatgcgtta gattgtaacg acaccaatca gcgtgacaac tgtcaggata gcagccagac
    20821 cgtagaaaac ccatttgccc gcaggtacgt ggattttcag atcgtgcatc gcgtggtgca
    20881 tacggtgtaa accacaccac agcggcagaa cgatcatcag gaacaggaat acgcgaccaa
    20941 tgaagctctg cgcgaacgcc agaacgcgct cgtagctcag tgcatcaccc ggaaacagcc
    21001 ccagcggcag cagaataccc accagcagga tcatcaccgg cgcaataatt gcgctccaca
    21061 taccaccggc cccgaagagg ccccagaata ccggttcgtc agaacgcttt ggatttggat
    21121 tgatcatctc aggctcctta ccagtacagg gcaacaaaca ggattacgat ggtggcaacc
    21181 acagttaccg cccagagact tttgataatt ggctctggac ccattttttc gtcttttaca
    21241 atgatattgg ccgctttcgg tgccagttca aaccaggttt tggtgtgcag cagagctgcc
    21301 gccagagtga tcaggttaat gatcacaata accgggtttt gtaaaaagtc gacgaatccc
    21361 gcccaggctt ccgggccatt tttcagggca aacagcccga aaatcagttc aatgctgaac
    21421 cacacagccg gaaccgccgt gccttcgcgc agcatgtaaa agcgataaaa cggcaatttt
    21481 ttccaccagg tggacgtcat tggccgtaca tacggtttac gtttagtcgt catgttgcac
    21541 tccttagcgt ggtttcaggg tcgcgataag aaagtctttc gaactttcta ctttgccctg
    21601 ctgaatggcc gcagccggat cgacgtgttt cgggcagact tcggagcaat agcccacgaa
    21661 agtacagctc catacgccgt tctggctgtt caactgcgcc atacgctcct tcttaccgtg
    21721 gtcgcggcta tcttcgttat aacgatgcgc cagcgtaatg gcagccggac cgatgaactc
    21781 tgggttcagg ccaaactgcg ggcacgcggc gtagcacaga ccacagttga tgcaaccgga
    21841 gaactggtga tacttcgcca tctgcgccgg ggtctggatg ttagtaccct gatccgcggt
    21901 gcgggagttg ccgatgatgt acggtttgat cgcttccaga ctttcgatga agtgggtcat
    21961 atcgaccacc agatcgcgtt caatcgggaa gttagctaac gcttcaacct tcataccgtc
    22021 ggtgtaatca cgcaggaagg ttttacatgc cagttttggc acgttgttaa ccatcatgcc
    22081 gcaggaacca caaatcgcca tacggcagga ccagcggtag ctcaggtccg gtgccaggtt
    22141 gtctttgatg tagcccagcg catccagtaa tgaggtagtt gcgtcataag gcacttcata
    22201 gaatgcgcta tgcggtgcgg tatcgacttc cgggttatag cgcaccacct caattttcag
    22261 gtttttcatc tcagccattc gccttctcct tcttattggc tgcttccgcc ttatcggctg
    22321 catccgcttc gccaccgtaa acgcgtttag ctggcggcag cgtagtaatc ttcacgtcgc
    22381 tgtactccag gcgagtcgtg ccatcagcat cgcggaaggc gagggtgtgt ttgaggaagt
    22441 tgacgtcgtc acgctcggtg caaccttcgt ccagacgctg gtgtgcgccg cgggactctt
    22501 tacgtgccat tgcggagtgc gccatacatt cagcaacgtt cagaccgtgg cccagttcaa
    22561 tggtgtagag caggtcggtg ttgaacacgc tggaagtgtc ggtgatgcgc acgcgcttga
    22621 agcgttcctg cagctcagcc agcttgtcga tggttttctg catcagttcc ggcgtacggt
    22681 agataccgca gccttcttcc attgccaggc ccatttcgtc gcggatcttc gcccagtttt
    22741 cgccgccatc ctggttaacc agatctttca gacgttgttc aacgccagct gcctgcgctt
    22801 caattgccgc ttcgttgcca ttaccggcag ttgctgcacg ctctgtcgct tgttcaccgg
    22861 ccagacggcc gaagaccacc agttccgcca gggagttgga gcccagacgg tttgcaccgt
    22921 gcagaccaac agaggaacat tcacccacgg cgaacagacc tttaatgcgg gtttcacagt
    22981 tctgatcggt ttcgataccg cccatggtgt agtgtgcggt cggacgtacc ggaatcggtt
    23041 ctttaaccgg atcgacgcca acgtacgctt tcgccagttc gcagatgaac ggcagacgtt
    23101 catgcagttt tttctcgccg aggtgacgca ggtcgagata aaccacatca ccacgcggcg
    23161 tggagatggt gttgccttta cgccattcgt gccagaaggc ctgagaaact ttgtcgcgtg
    23221 gacccagttc catatatttg tttttcggct cgcccagcgg agtttccggg cccatgccgt
    23281 aatcttgcag ataacggtag ccatttttgt tgaccagaat accgccttca ccacggcagc
    23341 cttcggtcat caggataccg gaacctggca gaccggttgg gtgatactga acgaattcca
    23401 tgtcacgcag cggaacgccg tggcttagcg ccatacccat accgtcaccg gtaacgatgc
    23461 cgccgttggt gttgtaacga taaacgcgac ccgcaccgcc ggtagccata acgaccgcgt
    23521 tagcacggat ctgcaccagc gtgccttcca tcatgttcat tgctaccagg ccgcgaacat
    23581 gaccatcatc aaccagaata tccagcacga aatgttcgtc aaaacgctgg atctgcggga
    23641 attgcagaga ggtctggaac agcgtgtgca gcatatggaa gccggtctta tcggcggcga
    23701 accaggtacg ctcgattttc atgccgccga agcgacgtac gttgacgcta ccatccgggc
    23761 gacggctcca tgggcacccc cacagttcca gttgggtcat ttcggttggg cagtggtgga
    23821 cgaaataatc cacgacatcc tgctcacaca accagtcgcc acccgctact gtatcgtgaa
    23881 agtgatattc gaagctgtca tgatcctgcg cgacagcggc ggagccccct tctgcagcaa
    23941 cggtatggct acgcatcggg tatacttttg agattagtgc gatttttgca tttggatttg
    24001 cctgcgcggc agcaattgca gcacgtaatc ccgcgccacc ggcgcctaca atggcaagat
    24061 cggcttgaaa ggtttgcacg acattcctcc agattgtttt tatcccacag ccccgtactt
    24121 cagggtaagt gcctgaaagt tacggtctgc gaacgctatt ccactgctcc tttataggta
    24181 caacagtata gtctgatgga taagtctgaa atttgacgag atcgattttt ttagtgcgca
    24241 agagggctaa attatcactg aagatgatta atttaattac taaaccatca gatcgtgctt
    24301 tttttagtca ctgcccaccg ttgtttgatt cctgcgtaaa atttgtctcg ccgtcgcgtt
    24361 gcgagtagac ttcgtgccct tgtcaaaaac tggagattta actatgagcg aaacggcatc
    24421 ctggcagccg agcgcatcca ttcctaactt attaaaacgc gcggcgatta tggcggagat
    24481 ccgtcgtttc tttgccgatc gtggagtgct ggaggtggag acgccttgta tgagccaggc
    24541 gacggtaacc gatattcatt tggtcccgtt tgagacacgt ttcgttggcc ccgggcattc
    24601 gcaggggatg aatctctggt taatgaccag cccggaatac catatgaaac gcctgctggt
    24661 tgccggttgt gggccggtat tccagctgtg ccgcagcttc cgtaatgaag agatggggcg
    24721 ttatcacaac cctgagttca ctatgctgga gtggtatcga ccgcactatg atatgtaccg
    24781 gttgatgaac gaggtggacg atctcttaca acaggtgctg gactgcccgg cagcagaaag
    24841 cctttcttat caacaagctt tcttgcgtta tctggaaatt gacccactct ctgccgacaa
    24901 aacgcaactg cgggaagtgg cagcgaaact ggatttgagc aatgttgctg ataccgaaga
    24961 agaccgcgac acgttgctac aattgctgtt tacctttggc gtagagccaa atattggtaa
    25021 agaaaaaccg acctttgtgt accactttcc agccagccag gcatcactgg cgcaaatcag
    25081 taccgaagat catcgggtcg ctgaacgctt tgaggtttat tataaaggta ttgagctggc
    25141 gaatggtttc catgaattga cggatgcccg tgagcagcaa caacgctttg aacaagataa
    25201 ccgtaagcgc gcggcgcgcg gtttgccgca gcaccccatt gaccagaatc tgattgacgc
    25261 cttgaaagtc ggtatgcctg actgttccgg cgtggcatta ggtgttgatc gtctggtgat
    25321 gttggcgctg ggcgcggaga cactggctga agtcatcgcc tttagcgttg accgggcata
    25381 attctgaaaa attactgaat taacagcgaa gaatggcgtg ataagcgcca ttctgtagca
    25441 tatgttttca tttttccgtt aaaggccaca atttaagact ggatatttga tatcatccag
    25501 gtatcaattc tgtattggtt tttacgctgc ctgctcaatg ttgcgcagtg cagttcagtg
    25561 ggcgcgtgtt atacacgcgc tgaaatgaag gatggtttca tgcctcacac gataaaaaag
    25621 atgagtctga taggactcat attgatgatc tttacttccg tatttggatt tgccaatagc
    25681 ccatcggctt attacttaat gggttatagt gcgattccct tttatatatt ttctgcattg
    25741 ttattcttta ttccattcgc cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa
    25801 gaaggtggta tctattcctg gatgaataat agtgtcggac cacgttttgc cttcattggt
    25861 acgtttatgt ggttttcctc ttatatcatc tggatggtga gtacctccgc gaaagtttgg
    25921 gtaccgttct caacattcct ctatggtagc gacatgaccc agcactggcg tattgccgga
    25981 ctggagccta cgcaggtggt tggtctgctg gcagtggcat ggatgattct ggtcaccgtc
    26041 gttgcttcta aggggattaa taaaattgcc cgcattactg cggtgggcgg tattgcagta
    26101 atgtgtctga atttagtatt gctgttagta agcattacta ttttgttatt aaatggtggg
    26161 catttcgcgc aggatattaa tttccttgca tcaccgaacc cgggttatca gtccggtctg
    26221 gcaatgctat cgtttgtggt atttgctatt tttgcctatg gcgggattga agcggttggt
    26281 ggtctggtcg ataaaacgga aaatccagaa aagaactttg ccaaaggtat tgtttttgcc
    26341 gctattgtta tttcaatcgg ttattcgctg gcaatatttt tatggggcgt cagcacaaac
    26401 tggcagcagg tattaagtaa tggttccgtt aacctcggca atattaccta tgtgctgatg
    26461 aagagcctcg gggtgacgct gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg
    26521 ctgggcgtat ggtttgcgcg tattaccgga ctttcgatgt tcctcgctta taccggtgcg
    26581 ttctttacgc tttgctattc accgctgaaa gccatcatcc aggggacgcc gaaagcgttg
    26641 tggccggaac cgatgacgcg cctgaatgcg atggggatgc cgtctatcgc catgtggatg
    26701 cagtgcgggt tggttactgt cttcatcctg ctggtttcgt ttggtggcgg taccgcatcg
    26761 gcgttcttta acaagctgac gctgatggcg aacgtgtcta tgacgcttcc ttacctgttc
    26821 ctcgcgctgg ctttcccatt ctttaaagca cgtcaggatc tcgacagacc atttgtgatt
    26881 ttcaaaacgc gtatgtcggc aatgattgcg acggtggttg tcgtactggt ggtgacattt
    26941 gcgaacgtct tcaccatcat tcaacctgtg gttgaagccg gagactggga cagcacattg
    27001 tggatgattg gcggccctgt cttcttctcg ctgttagcga tggcgattta ccagaactat
    27061 tgcagtcgca tggcgaataa acctgagtta gctctcgact gatcatatga aatcctgcaa
    27121 ctcatcgtct ctccggcgat gagttgtcag gaagtaatgc gtatggatga tactctccag
    27181 ggccggtgat aactgaagat gatgttaagc tgctaaatga gccacgttga catcatttgg
    27241 cagcttcatg cattacaaac ttcccgcctg acgacctttg cccgcagacg tcagcgttct
    27301 ccccgtttgt ttaccgttga ggctttccag acgcatctgg aagggcggga atggcatatc
    27361 gataccgtgg gcatggaagc ctgccagaat cagctggtgg atctcatggc gtagcggcat
    27421 acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa atctgaatcc cctgttgcag
    27481 atccaccagg aagacttccg gtgccgggtt gtcgatcacc agcgagcagc gacgcgctgc
    27541 ggtgagcagg atttccgtca cttcttcgct attggcatcg gcaggggccg gtatcgtcaa
    27601 caccacgcgc gtgaccgagt cagagagcga ccagttgata aactgctcgg taataaacgc
    27661 cttgttcggc acgattatct ctttacggtc ccagtcgctg atggtggtgg cgcgggtgtt
    27721 aattttcgtc acgctaccgg tgagatcgcg aattgtcacc gtatcgccaa tgcgaatcgg
    27781 tttttcgaac aggataatca gaccagagat aaagttggcg aaaatttcct gcaaaccaaa
    27841 accgagacca acaccgagcg cggcaaccag ccactgcaat ttcgaccact caataccaat
    27901 cattgagaag ccgaccagcc cgccaatcag catcagcaga tattttgtga tggtggtgat
    27961 ggcgtaaccc gtacccggcg ttaaatccag gtgctgcaaa atcgccagtt ccagcagcgc
    28021 gggcaggttg cgcaccagct gcgtggtgat gataaatacc agaatggcaa tcagcaccgc
    28081 accgagggta attggctcca gactttctac gccctgtacc gtggaggtga catcccacag
    28141 cgaaatattt tcgaggaagc cgaaagcgga atggatttct gaccacagca caatgacaga
    28201 aagcagggcg atcaacatca aaattgagcg caccagccgc aaggattgcg cactgatggc
    28261 atcgagatcg acttcgcttt catcgacttc aattgctcct tccgggctac tgtgatgatg
    28321 cgcttcctct tcgccacgcg cacgttgcgc taacatctct gcccggcgat gcttcgcccg
    28381 atcaaacgcc agcctgcggc gctggatcag catccagcgg cggataacgt gataaaccac
    28441 cagtagcagg aaccagatgg caaccgaggt ttcaagcctc gctaacagcg cctgtgccgt
    28501 tgccagataa cccaccgccg acgccagaat ggcaaccaac ggcgcgccaa tcatcatgtt
    28561 ccacagcata tggttggtaa tgttgtcgcc gctgccctct ttgttgagat acagcggaat
    28621 cccggccttt ttcaggctga gggtgaccac cgccagcgca ccgcaaatga ggataaagca
    28681 aagccgtccc agcgaaccgg agaattcacg gtcgtcgagg ttatcgaaca tcatcagcgc
    28741 cataatcagc ggcacaataa gcccgatgct catcaggtag tagcgcatcc cacgggaaac
    28801 acgttcgcgc ggccagccaa aatgagcgat aaacaagccg ttcgggcggg caaaggtggc
    28861 gcaaatcatc actacccaca gcagcggcac ggtggccgtt acaccatcgc caatcgcgac
    28921 cgccagcgga taaggccacg cctcgcgcaa gccgtaaccc agcgtcatcc acagcaccgg
    28981 taacggtgac gcgacgagaa tcgaccagaa aagagtgcgc aacgtcagcc agaagtgatc
    29041 ctgagtcact ttgccaactt tcgccgccga acgttcaaga aaacgggtga aatagcggcg
    29101 cgagtaaata ctgcaaccga ccagaatcaa cgcgccaaac agcggcaaaa ttgtctcttt
    29161 gctggtcagc atcatcacac tggctttgcc caactgactg aaggtgtcca gcgaaatgag
    29221 acgacgcaga tcctgggcga tttccagcgg ccaggcgatg gtcatcgggc gcacgtcaga
    29281 ggtccagaac agatagcggt gcgttgcttc gttcacctct ttcagcgcat cctccagttg
    29341 cccgttggag actttcagct tggtcagttc caatagtagc gtgtcgccac cctgcaacaa
    29401 tgagttcagc aactcacgct gagtgcgcag ttgtgcttcc agaatacggt tttgctcggc
    29461 agtcagcggc tgaccgtcgg cctgatgaat ttgccgtagc agcggctgtt tattaagcag
    29521 atcctcataa cgtaaccgtt gcacacgcaa ctgcgccatt tcggtatcaa gctgttgtgg
    29581 tttcggcatt tccggcagcc gtgccacctg cgcccgcagc gcttcgccga gcagattgga
    29641 cgatcccagc cattgcgact gttcacgcag cgtattcaac gcctgccgga cctgtaacgt
    29701 ctggctggca gcctgacgct gttgcgaggc aacgagatcc atccgctgcg cctgttgatt
    29761 caaagccgcc gatagttcgc ggttaatttt gaattgcgcg acgatatctt tcggcaaatc
    29821 ggcgctgttt tctgccagca attcggtact ttccagcgcc cgctccgcct caagctgacg
    29881 ttggctgttt aattgattac gcaaggcctg caaatacgca tccagttgct ggctctcttt
    29941 ttccgccagc tctgagcgta agcgcgctaa ttcctggcgg ttattggcag acagctgcgc
    30001 cagctccagt tcatcaacga gcgccttaag acgtgcagag tcagactgca acgcgaaatt
    30061 ttgtgcctga ttgagcggag tattgccggt aagcgttccc aggcggcgct cgatctcatt
    30121 taactgacgg cgggcgtcgg tttgctgttg cggcagttga ttcagcgaat cggcaatctc
    30181 gcgggcgcgc tcctgctctt gctgggcctg acggctttta tccagcaact ggctgctgac
    30241 ctggagaatt tcctgattca gcgcgtcggt agacattccc ggcgacacgc tgcgcggctc
    30301 gtcacgcatg ttgtttaatt gtgcgcgcag agtagcggag agtttcggat aattatcgat
    30361 aacttgctga tattgtttga tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa
    30421 ggcagactgg agcgcctcta cgacttccgg ctgtgcgggt ttcgccgctt ttgcctgctc
    30481 cagttcctga gtgatttgtt tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag
    30541 gcaccaggcc atcagaaaag tgataatcag gcgcacgtca gcgtttcctt tgatggatta
    30601 gacctggtct tttttgtcgt caaccaatgg gctggcgtcg tgttctgctt cgatctcttc
    30661 agcaggaagc ggggcaggtt cagcgtctgg cgtaacaaag gtttcggtag atactgccag
    30721 cggctggcca attttcgtga cagacaggct ttccagttgc tcaaccagat tcactttacc
    30781 cggtgcaaac aggttgataa cggtggaacc gagtttaaag cgacccattt cctggccttt
    30841 cagcagtgcc acagaaccgt cgttttcccc ggcaggccag gtccagcgct tgatgatacc
    30901 ttcgcgcggc ggcgtaatgg tgcccgccca gaccgtctca atgctgccaa caatcgtcgc
    30961 tccgaccaga atctgcgcca ttgggccaaa ttcggtatcg aaaaggcaaa tcacgcgttc
    31021 gttacgggca aacagattcg gcacgttctg agccgtgaga tggttaacgg agaagagatc
    31081 gcccggcacg tagatcatct cacgcagaat accgttgcac ggcatgtgta cgcggtggta
    31141 gtcacgcggg gagaggtaag tggtcacaaa cgtaccgttg cggaacaggt ccgccatcag
    31201 atagttgcct gccagcaggg cttcgaggct gtagttgtgg cctttggctt gcaggatttt
    31261 atcttcttcg attttaccca actggctgat aacgccatcg gcaggcatga ccagtacatt
    31321 cggatcggta tcgattgggc gtacttcgtc acgcagcgga cggacaaaga attcgttaaa
    31381 ggtgcggtag ctggcggtgt ccggcttttg cgcctctttc atgtcgacct tgtagtattt
    31441 aacgaacaga tcgataacca gttttgtcag ccatcctgcc cgcttgcttg cgccccaacc
    31501 cgccaggcga gtaagccata gtttcggcag aatgtactgt agcgaaagtt taaatgaatt
    31561 taacaaggta gcctccaggc cattgttttg tcgttcctga tccggcctac atgccggatc
    31621 ctgaaaaaaa ggggacgatt ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga
    31681 aaagttttta cgcgttttta cctgcgccat gctttccaga atacggtgat agttttcgaa
    31741 acgggtttcc gcgattttcc cttcctcaac cgcttcccgg atagcgcagc ccggatcggt
    31801 atcgtgtttg caatcgcgat atttacacag acctaaatag tcatggaatt cgacaaagcc
    31861 ctgagtgatt tgttccggct ccaggtgcca gaggccgaac tcacgcactc ctggggagtc
    31921 aatcacatca ccgccgtgcg ggaagtgata cagccgagcg gcggtcgtgg tgtgctgacc
    31981 gagccccgag ttgtcagaga tatcgttggt caggatctct ttttgcagcc ccagcaacgc
    32041 attcagcagg ctggatttgc cgacgccaga ctgcccggca aaaatgctga tgcgcccggt
    32101 caacgcctct tccagcggtt ttagcccatc ctgagtatgg ctggaaacca tcaatacgcg
    32161 ataaccgata ttgcggtaga tatccatctg ctcgttgacg aacgccatgc cttcgtcgtc
    32221 cagcaggtct atcttgttga gcacaataat cggctcaatc tgcaaggttt cgcaggccac
    32281 caggtaacgg tcgataatat tgagcgacag ctccggcaaa atggcggaga caatgacaat
    32341 ctggtcgatg ttggcggcaa taggtttcac gccgtcgtag aaatccggac gcgtcaacac
    32401 cgaggtgcgc tcatgcaccg cttccacgat ccctttgaca tttacgcctt ccgccgccgg
    32461 tttacccgga cgccagacta cgcggtcgcc ggtcaccagc gaacggatgg tacggcgaat
    32521 attgcagcgg tgaacgtcgc catcggcgga ttccacatca gcgtgcatac caaagcggct
    32581 gatgacgata ccttcatcag gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt
    32641 agacgtttta agacgacgct ggtgattggc gttcacgcgg cgctgctggc ctttggagag
    32701 tttattttta ctcaatcgta cagactcctg gtcgcccctg atgggcaaaa catctatgat
    32761 acacgcaatt gtggatcaat atagtcactg tgaatgggtg gaaaatagca tgagtgccaa
    32821 tgaaaacaac ctgatttgga tcgatcttga gatgaccggt ctggatcccg agcgcgatcg
    32881 cattattgag attgccacgc tggtgaccga tgccaacctg aatattctgg cagaagggcc
    32941 gaccattgca gtacaccagt ccgatgaaca gctggcgctg atggatgact ggaacgtgcg
    33001 cacccatacc gccagcgggc tggtagagcg cgtgaaagcg agcacgatgg gcgatcggga
    33061 agctgaactg gcaacgctcg aatttttaaa acagtgggtg cctgcgggaa aatcgccgat
    33121 ttgcggtaac agcatcggtc aggaccgtcg tttcctgttt aaatacatgc cggagctgga
    33181 agcctacttc cactaccgtt atctcgatgt cagcaccctg aaagagctgg cgcgccgctg
    33241 gaagccggaa attctggatg gttttaccaa gcaggggacg catcaggcga tggatgatat
    33301 ccgtgaatcg gtggcggagc tggcttacta ccgcgagcat tttatcaagc tgtaaaattt
    33361 ttcctggtca cgtaagcgcc ggaatccact tgcggcggcg tgattttgac gctaaattga
    33421 acgtcttgct gataatttca gcacttgaga taaaaacgca aaaaaaactt ttttgggggg
    33481 ttgcagaggg aaagatttct cgtataatgc gcctcccgta acgacgcaga aatgcgaaaa
    33541 ttacgaaagc aaaattaagt agtacgcggg aatagctcag ttggtagagc acgaccttgc
    33601 caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttgaa aagtgctgca
    33661 aagcacagac cacccaagcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg
    33721 gggtcgc
//
LOCUS       DAZFZC010000047        32963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFZC010000047 DAZFZC010000000
VERSION     DAZFZC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.047"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(154..1746)
                     /gene="lsrK"
                     /locus_tag="ACHKXP_002614"
     CDS             complement(154..1746)
                     /gene="lsrK"
                     /locus_tag="ACHKXP_002614"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO7647592.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGSLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1825..2778)
                     /gene="lsrR"
                     /locus_tag="ACHKXP_002615"
     CDS             complement(1825..2778)
                     /gene="lsrR"
                     /locus_tag="ACHKXP_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO7647593.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRC"
     gene            3026..4561
                     /gene="lsrA"
                     /locus_tag="ACHKXP_002616"
     CDS             3026..4561
                     /gene="lsrA"
                     /locus_tag="ACHKXP_002616"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO7647594.1"
                     /translation="MQTSDTCALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFRRLQELLSTGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHVAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4555..5583
                     /gene="lsrC"
                     /locus_tag="ACHKXP_002617"
     CDS             4555..5583
                     /gene="lsrC"
                     /locus_tag="ACHKXP_002617"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO7647595.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLFA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNIRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5583..6575
                     /gene="lsrD"
                     /locus_tag="ACHKXP_002618"
     CDS             5583..6575
                     /gene="lsrD"
                     /locus_tag="ACHKXP_002618"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO7647596.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLVRRANNPLP"
     gene            6587..7609
                     /gene="lsrB"
                     /locus_tag="ACHKXP_002619"
     CDS             6587..7609
                     /gene="lsrB"
                     /locus_tag="ACHKXP_002619"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO7647597.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7636..8511
                     /gene="lsrF"
                     /locus_tag="ACHKXP_002620"
     CDS             7636..8511
                     /gene="lsrF"
                     /locus_tag="ACHKXP_002620"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO7647598.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8535..8825
                     /gene="lsrG"
                     /locus_tag="ACHKXP_002621"
     CDS             8535..8825
                     /gene="lsrG"
                     /locus_tag="ACHKXP_002621"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO7647599.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8882..9640
                     /gene="tam"
                     /locus_tag="ACHKXP_002622"
     CDS             8882..9640
                     /gene="tam"
                     /locus_tag="ACHKXP_002622"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO7647600.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9644..10558)
                     /locus_tag="ACHKXP_002623"
     CDS             complement(9644..10558)
                     /locus_tag="ACHKXP_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO7647601.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFFIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10765..12216)
                     /gene="uxaB"
                     /locus_tag="ACHKXP_002624"
     CDS             complement(10765..12216)
                     /gene="uxaB"
                     /locus_tag="ACHKXP_002624"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO7647602.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12443..13861)
                     /locus_tag="ACHKXP_002625"
     CDS             complement(12443..13861)
                     /locus_tag="ACHKXP_002625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7647603.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14000..14359)
                     /locus_tag="ACHKXP_002626"
     CDS             complement(14000..14359)
                     /locus_tag="ACHKXP_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO7647604.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14359..15285)
                     /gene="glsB"
                     /locus_tag="ACHKXP_002627"
     CDS             complement(14359..15285)
                     /gene="glsB"
                     /locus_tag="ACHKXP_002627"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO7647605.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15349..16737)
                     /gene="sad"
                     /locus_tag="ACHKXP_002628"
     CDS             complement(15349..16737)
                     /gene="sad"
                     /locus_tag="ACHKXP_002628"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO7647606.1"
                     /translation="MTITPATHAISINPANGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALLARNEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNNADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16838..17713
                     /locus_tag="ACHKXP_002629"
     CDS             16838..17713
                     /locus_tag="ACHKXP_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7647607.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            17797..18912
                     /gene="yneK"
                     /locus_tag="ACHKXP_002630"
     CDS             17797..18912
                     /gene="yneK"
                     /locus_tag="ACHKXP_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO7647608.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPARNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLPSDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQNLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            19062..20252
                     /gene="ydeA"
                     /locus_tag="ACHKXP_002631"
     CDS             19062..20252
                     /gene="ydeA"
                     /locus_tag="ACHKXP_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO7647609.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20277..20942)
                     /gene="marC"
                     /locus_tag="ACHKXP_002632"
     CDS             complement(20277..20942)
                     /gene="marC"
                     /locus_tag="ACHKXP_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO7647610.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21154..21588
                     /gene="marR"
                     /locus_tag="ACHKXP_002633"
     CDS             21154..21588
                     /gene="marR"
                     /locus_tag="ACHKXP_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO7647611.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21608..21991
                     /gene="marA"
                     /locus_tag="ACHKXP_002634"
     CDS             21608..21991
                     /gene="marA"
                     /locus_tag="ACHKXP_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO7647612.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22023..22241
                     /gene="marB"
                     /locus_tag="ACHKXP_002635"
     CDS             22023..22241
                     /gene="marB"
                     /locus_tag="ACHKXP_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO7647613.1"
                     /translation="MKPLLSAIAAALILISAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22298..23737)
                     /locus_tag="ACHKXP_002636"
     CDS             complement(22298..23737)
                     /locus_tag="ACHKXP_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7647614.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWSEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLRFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23762..25435)
                     /locus_tag="ACHKXP_002637"
     CDS             complement(23762..25435)
                     /locus_tag="ACHKXP_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO7647615.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     VTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYMTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDEQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25491..25802)
                     /locus_tag="ACHKXP_002638"
     CDS             complement(25491..25802)
                     /locus_tag="ACHKXP_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO7647616.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25830..27152)
                     /locus_tag="ACHKXP_002639"
     CDS             complement(25830..27152)
                     /locus_tag="ACHKXP_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO7647617.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQSIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGVVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27267..27578)
                     /locus_tag="ACHKXP_002640"
     CDS             complement(27267..27578)
                     /locus_tag="ACHKXP_002640"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7647618.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     Q"
     gene            27777..28475
                     /locus_tag="ACHKXP_002641"
     CDS             27777..28475
                     /locus_tag="ACHKXP_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7647619.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIKKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28520..29419)
                     /gene="eamA"
                     /locus_tag="ACHKXP_002642"
     CDS             complement(28520..29419)
                     /gene="eamA"
                     /locus_tag="ACHKXP_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO7647620.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29614..30801
                     /gene="ydeE"
                     /locus_tag="ACHKXP_002643"
     CDS             29614..30801
                     /gene="ydeE"
                     /locus_tag="ACHKXP_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO7647621.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYNLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            30928..31023
                     /gene="mgtS"
                     /locus_tag="ACHKXP_002644"
     CDS             30928..31023
                     /gene="mgtS"
                     /locus_tag="ACHKXP_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO7647622.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            31023..31127
                     /gene="mgtT"
                     /locus_tag="ACHKXP_002645"
     CDS             31023..31127
                     /gene="mgtT"
                     /locus_tag="ACHKXP_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO7647623.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(31242..32132)
                     /gene="dgcZ"
                     /locus_tag="ACHKXP_002646"
     CDS             complement(31242..32132)
                     /gene="dgcZ"
                     /locus_tag="ACHKXP_002646"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO7647624.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADLAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(32387..32779)
                     /gene="ydeI"
                     /locus_tag="ACHKXP_002647"
     CDS             complement(32387..32779)
                     /gene="ydeI"
                     /locus_tag="ACHKXP_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO7647625.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDHYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         8630 a         8126 c         7651 g         8556 t
ORIGIN      
        1 aaaatagcaa tttccccttg aatatattac tggtgatgga aggatgaatc tgcaagctgg
       61 catttaacaa gccagttcta aaagaaggga aataagaata actatactca aaaactaaca
      121 gccacggtca tcatgatgtg gctgtcaatg aaactataac ccaggcgctt tccataacga
      181 cgtcgtcagt ccatgatcaa ccagccccag ctgatcctga taaactgcct gccacttatc
      241 gcgtgaatcc tgataaagtt catgcttttc cgggtctggt gtgtgcgtcc gttcccagcg
      301 aaccaggcgt tctccggttt ctgccattga tgaaaaaatt ccggcaccga cgccagctgc
      361 aatggcacat cctaatgcag tggcttcttt gaccaccgga atattgacgg gtaatcccga
      421 gacatcagcg agaatttgac tccataattt cccttttgaa cctccgcctg caaagactaa
      481 cgatgaagga tgaatattcg agaaatcagc aatttgctgc aagttacacg ctgatacaat
      541 cgccgcattt tcttccagcg cacggaacaa tgtcgctttg ttacatttat ccgggtcaat
      601 ggacaagtta ataaaggaag gcgcagcgtg ataccaggtt ttaaagcgca ttctgtcgga
      661 gaatatcggc attacgcccc acgacccagg cggcacccgg ctggccatct cttccagcag
      721 cgtataggtg tcgatgccta aacgttcggc aatcagtttt tcttcggcac agaaagcatc
      781 gcggaaccag cgcatggtga gtccggtaaa aaagcttata gattcagctt gtaccatgcc
      841 aggaataaca tgaggattaa cgcgcacgtt catttctggg tctgtcaccg gcgcggctaa
      901 atttacaact tgctgccaga atgtgccgcc aagaaccgcg gtttgtgccg gacgcacaac
      961 gcctaaccca aggctaccaa gctgcacgtc gcctcctcca acgaccaccg gagtgcctgc
     1021 cttcagaccg cagagttccg ccgcttgtga acttaccacg cccagcaatg tgccggtttc
     1081 tttgacagga gaaagaatat cggcacgcag gccagccata tccagcaatg caggtttcca
     1141 gtcacgggtg gttagatcaa gaagtcccgt ggtgccagcg ttagagggat ccaccgccag
     1201 ttcgccgctg agcatatagg ccagccagtc gctgatcatg gtgatggttg atgcctgacg
     1261 gtaaatatcg gaacgatggt gcgccagcca aagtaatctg gggatggcac ttaaagccag
     1321 tgtttgtccg gtcgcgcgat aaacttcgtt ttcaaaggta ttgttgtgca gttctttaag
     1381 ttcgctaact tcgcgcgccg ctctggcatc cacattggcg caggcccaga tcggtgttcc
     1441 ttcattgtta tataaaacaa tgccttcacg catagaacat gccgaaacgg cagcgatata
     1501 ctccgggtct atgccggcgt tgtgcagcgc ctggcgcata cactcacacg ccagttgcca
     1561 gtttttgttg agatcaaatt ccatagaacc aggaacgtcc ggtactgcca gatgccgcca
     1621 ctccgcctgt cccactgcta tttgattgcc ttccaggtcg aatatcacag cccgaatact
     1681 tccggtgcct gcatccagcg ccatcaggta gtactttgat tctgaagggg taaagagtcg
     1741 agccattaga ttatcctcgg cttatgcgtt ctgtctgttc ctctatacgt tctccatcat
     1801 tcccggtaat aaggtcatgc aaatttaaca acgtaaaatc gccgctgctg tgtcctgatc
     1861 ggtaaccagt gcgttgatat aaccgccttt cattgcagcg gcaattgctt cggctttatt
     1921 ttctccccct gccacgccaa cccggacggg tatggtcttc agcgcgctta aaggtaagcc
     1981 aatcagttcg ttatgtattt tgatatccgt gacaacgtca ccttttgcat caaaaaagta
     2041 gcctaaaatg tcgccaaccg ccccttttcg gccaatcatt aactgttcgc cctggctgat
     2101 ataaccggag cgaatgatcg tcgcgtcgtc ttgttgactc acggccccaa tgccgacaat
     2161 cgccacatcc gctgcttgcg cggctagcag aacatctttg acgcaatttt cattttttag
     2221 cgtacgggca atgtcagcgg aggatgcccg caacggagcc ggaataatat tcacactgca
     2281 cgccgcgtta agctgcccga tacccgtcat ataagaaccg acgccaccgg agagcgtgac
     2341 caggcgaatt tgctgtgacg aaataaaacc acttaagcgt tgcagcgtat tcatggttgc
     2401 ctcgccaaaa ccaatcgcca gcatctgttg tggttgaagt aaactcatca acatatgcgc
     2461 cgcgcctatc cctagtcgcc cgccgacatc agcatccgca agcccaggga tcacccggac
     2521 atgttgcagc gaaaactgac gacgtaattg tgtttcatat tccagacagc cttcaaagcg
     2581 agaattaatc tgcacgcgaa tgatgccgga ctgatgccct ttctccagta atcgcgacac
     2641 tttcaaacgg gtcaggccga gacgatcgct gatctcactc tgggtcagcc cgtcgtggta
     2701 gtaaaaccac gcgatccgcg cgacctgttc ttcttcacac attccctgtt ctgaaattac
     2761 tgaatcgttg attgtcataa ttcattcttc actttgaaca tatttaaatc tttaatgcaa
     2821 ttgttcagtt cttggcattt atatctgtga tggcaaccac agtttgactc tacgagcatg
     2881 aacaaacgca accgtgaaaa tcaaaatagc ataaattgtg atctattcgt cggaaatatg
     2941 tgcaatgtcc acctaaggtt atgaacaaat taaaagcaga aatacatttg ttcaaaactc
     3001 acctgcaaaa ctgaacgggg gaaatatgca aacgagtgat acctgcgcgt taccgctact
     3061 ttgcgcccgc tcggtttata aacagtattc aggggtcaat gtcctgaaag gcatcgattt
     3121 tacgttgcat cagggggagg tccacgccct gctcggcggc aatggtgccg gtaaatcgac
     3181 gttaatgaag attattgccg gtattacccc tgctgatagc ggtacgctgg agattggagg
     3241 caacaactac gtcagattaa cgccagttca tgctcatcag ctgggtattt atctcgttcc
     3301 ccaggaaccg ctgcttttcc caagcctgtc gataaaagaa aacatcctgt ttgggctggc
     3361 aaaaaaacag ctctccatgc agaaaatgaa gaacttgctg gcggcgctgg gctgccagtt
     3421 tgatctgcat agtctggcag gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct
     3481 ccgcgggctg atgcgcgact cgcggattct gatcctcgat gaacctaccg cctcgcttac
     3541 ccctgcggaa accgaacgct tgtttcgtcg cttgcaagag ttgctttcta ctggcgtggg
     3601 tattgttttt atctcgcata agctgccgga aattcgccag attgccgatc gaattagcgt
     3661 gatgcgcgac ggaaccatcg ccttaagcgg caaaaccagc gaactgtcta ccgacgacat
     3721 tattcaggcc atcactccag tggtacggga aaaatcgctc tctgccagcc aaaaattatg
     3781 gctggagtta cctggtaacc gcccacaaca tgtcgccgga acgccggtgc tgacactgga
     3841 aaatctgacc ggcgaaggtt tcaggaatgt cagcatgacg ctcaatgccg gagaaattct
     3901 gggcctggct gggctggtgg gggccggacg cacagaactg gccgagacgc tctatggtct
     3961 gcgtactttg cgtggcggac gcattatgct gaatggtaaa gagatcaata aattatccac
     4021 tggagaacgt ttactgcgcg gtctggttta tctgccggaa gatcgccagt catccggact
     4081 gaatctcgat gcttcgctgg cctggaacgt ctgcgccctt actcataacc ttcgtggatt
     4141 ctgggcgaaa accgcgaaag ataatgccac cctggaacgt tatcgtcggg cgctgaatat
     4201 taaatttaac caaccggaac aagctgcacg gacattatcc ggtggcaacc agcaaaaaat
     4261 cctcattgcc aaatgcctgg aagcttcgcc gcaagtattg attgtcgatg agccgacgcg
     4321 cggcgtggat gtctcggccc gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca
     4381 aaatgtggct gtgctgctta tctcctccga cctggaagag atcgaactga tggcagatcg
     4441 tgtgtatgtg atgcatcagg gcgaaattac ccactctgca ctgaccgggc gcgatattaa
     4501 tgtcgagact attatgcgcg ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg
     4561 aagtttattc agaacaaccg tgaaatcacg gcactgctgg cggtggtgct gctgtttgta
     4621 ttacccggtt ttctcgaccg ccagtattta agtgtgcaaa cgctgaccat ggtttatagc
     4681 agcgcgcaaa tcctgatcct gctggcaatg ggcgcgacgc tggtaatgct tacgcgcaat
     4741 attgatgttt cagtgggttc gattaccgga atgtgcgcgg tgctgttggg gatgttactg
     4801 aacgcaggat attcactacc tgttgcttgt gtcgcgactt tactgcttgg tttgttcgcg
     4861 ggatttttca acggtgtcct ggtcgcgtgg ctaaagatcc ctgccattgt tgccaccctt
     4921 ggcacgttag ggttgtacag aggcatcatg ttgctgtgga ctggcggtaa atggattgaa
     4981 gggttacccg ctgaactgaa acagctctcc gccccgctgc tgcttggcgt ttcagcaatt
     5041 ggttggttga cgataattct ggtggcattt atggcctggc tgctggcgaa gacggcgttt
     5101 ggacgcagtt tttatgccac gggcgataat ttacagggcg ctcgtcaact gggcgttcgt
     5161 acggaagcca ttcgcattgt ggcattttcg ttgaacggct gcatggcggc actggcggga
     5221 attgtgtttg cttcgcagat tggttttatc cccaaccaga ccggtaccgg gctggagatg
     5281 aaagcaattg cagcctgcgt gctgggcggc attagtttgc tcggcggttc cggtgcgatc
     5341 atcggtgcgg tactcggcgc atggttcctg acgcagatcg atagcgtact ggtgctgttg
     5401 cgcattccgg catggtggaa cgattttatc gcgggcatgg ttctgctggc ggtgctggtg
     5461 tttgatggac gcctgcgttg tgcgctggaa cgtaatatac ggcggcaaaa atatgcccgc
     5521 tttatgacgc caccgccatc cgttaaaccc gcttcgtcag gtaaaaaacg ggaggccgca
     5581 taatgcgtat tcgctacggt tgggaactgg ctcttgccgc actgctcgtt attgagattg
     5641 tcgcatttgg tgcaattaac ccgcggatgt tagatctcaa tatgttgctg ttcagcacca
     5701 gtgactttat ctgcattggc attgtcgccc taccgctgac gatggtgatt gtcagtggcg
     5761 ggatcgatat ttcgtttggt tcgaccatcg gcctctgcgc cattgcattg ggcgtactgt
     5821 ttcaaagtgg tgtgccgatg ccgctggcga tactcctgac cttactgctc ggcgcattgt
     5881 gcgggctgat caacgccgga ttaattatct ataccaaagt taacccgctg gtgattacgc
     5941 ttggcacgct gtatctgttt gccggaagtg ccctgctgct ttccgggatg gccggagcga
     6001 cggggtacga aggtattggt ggattcccga tggcgtttac agatttcgct aacctcgatg
     6061 tgctgggact ccccgttccg ctgattatct ttctgatatg tctcctcgtt ttctggctct
     6121 ggctgcataa aacccatgcc ggacgtaatg tgtttttgat tgggcaaagc ccgcgcgtgg
     6181 cgctttatag cgcgattcct gttaaccgta ccttatgtgc gctctatgcc atgacggggc
     6241 tggcgtctgc ggtcgccgct gtgctgctgg tgtcgtattt tggttcagca cgttccgatc
     6301 tcggtgcgtc atttctgatg cccgccatca ccgccgtggt gcttggcggg gccaatattt
     6361 atggtggttc cggttccatt atcggcaccg ccattgcggt tttattagtg ggatatttgc
     6421 aacaaggttt gcaaatggca ggagtgccaa atcaggtgtc cagcgccctt tccggtgcgc
     6481 tacttatcgt cgttgtcgta ggtcgttccg ttagcctgca tcgccagcaa attaaagagt
     6541 ggctggtgcg tcgggccaat aacccattgc cataaaggat atcttcatga cacttcatcg
     6601 ctttaagaaa atcgccttac ttagcgctct tggcattgcc gcaatctcta tgaatgtgca
     6661 ggccgcagag cgtattgcat ttattcccaa actggttggc gtgggatttt ttaccagcgg
     6721 tggcaacggc gcacaacaag cgggtaaaga gctgggcgtt gatgtgacct acgacgggcc
     6781 gacagaaccc agtgtttctg gtcaggtaca gttgattaat aacttcgtca atcagggtta
     6841 taacgccatt atcgtttctg cggtttcgcc tgatggcttg tgtccggcac tgaaacgcgc
     6901 catgcaacgt ggtgtgagag tgctgacctg ggactctgat actaaaccgg agtgccgctc
     6961 ttactacatt aatcagggaa cgcccgccca gttgggaggt atgttggtgg atatggcggc
     7021 gcgtcaggtg aataaagaca aagccaaagt cgcgtttttc tactcaagcc ccaccgttac
     7081 ggaccaaaac cagtgggtga aagaagcgaa agcgaaaatc gccaaagagc atcctggctg
     7141 ggaaattgtc actacgcagt ttggctataa cgatgccact aaatcattac aaaccgcaga
     7201 aggaatatta aaagcgtata gcgatctcga cgccattatc gcccccgatg ccaatgccct
     7261 gcccgctgcc gcacaagccg cagaaaactt gaaaaatgac aaagttgcga ttgtcggatt
     7321 cagtacgcca aatgtgatgc gtccatatgt ggaacgcggc acggtgaaag aatttggcct
     7381 gtgggatgtg gttcagcaag gcaaaatttc agtgtatgtc gcggatgcat tattgaaaaa
     7441 aggatcaatg aaaacgggcg acaagctgga tatccagggc gtaggtcagg ttgaagtctc
     7501 gccaaatagc gttcagggct atgactacga agcggatggt aatggcatcg tactgttacc
     7561 ggagcgcgtg atattcaaca aagagaatat cggcaaatac gatttctgat gtgcattact
     7621 taaccggagt aagttatggc agatttagac gatattaaag atggtaaaga ttttcgtacc
     7681 gatcaaccgc aacaaaatat cccttttacc ctgaaaggtt gcggtgcgct ggattgggga
     7741 atgcagtcac gcttatcgcg gatatttaat ccgaaaacgg gtaataccgt gatgctggcc
     7801 tttgatcatg gttattttca ggggccgact accggacttg aacgcattga tataaatatc
     7861 gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt gcgcagcgta
     7921 gttcccccag cgaccaataa gccggtggta ctgagggcgt caggtgcgaa ctctattctg
     7981 gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg cctgaacagt
     8041 tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc gatcaaaaat
     8101 attattcagc tggttgatgc cggaatgaaa gtgggaatgc cgaccatggc cgtgactggc
     8161 gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg aatcgccgct
     8221 gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga acggattgtt
     8281 gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga gcgcgaggcg
     8341 ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat ggggcgtaat
     8401 attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt ggttcaccat
     8461 aacgaaacgg ctgatcgagc atatgaactc tatctgagtg aaaaacagta actgcggatc
     8521 taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa gacaaggttg
     8581 acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa gaaggcaatt
     8641 tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatc tacgaagcct
     8701 ataaagatga agacgcagtg gcgttccata aaaccacgcc ccactacaaa acctgtgtcg
     8761 cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat ggtttgatgc
     8821 cgtgaggcga atttatcaat tttatctaca attggggtaa cgcgctgacg ggagtaaaaa
     8881 aatgtctgac tggaacccct ctttatattt gcacttttcc gctgaacgat cgcgtccggc
     8941 ggtggagctg cttgccagag tgccgctgga aaatgtcgaa tatgttgccg atcttggctg
     9001 tggcccaggt aacagcaccg cccttctaca acaacgttgg cctgcggcca ggataacagg
     9061 catcgactcg tctccggcga tgattgctga agcgcgcagt gctttgccag actgccagtt
     9121 cgtggaagca gacatccgca actggcaacc ggtacaggca ctcgatctga tttttgctaa
     9181 tgcctcactg caatggctgc ccgaccacta cgaattgttt cctcatctgg tttctttact
     9241 taatccgcag ggcgtgctgg cagtacagat gccagataac tggctggagc cgacccatgt
     9301 gctcatgcgc gaagttgcct gggaacaaaa ctacccggat cgcgggcgtg agccgttggc
     9361 tggcgttcat gcttactacg atattttgag cgaagccgga tgtgaggtcg atatctggcg
     9421 aaccacctac tatcaccaga tgccgtcaca ccaggcgatt atcgattggg tgactgccac
     9481 tggattacgt ccgtggttac aggatctgac cgagagcgaa cagcagcttt ttcttaagcg
     9541 ctaccatcag atgctggaag agcagtatcc actgcaagag aacggacaga tactgctggc
     9601 atttccgcgt ctgtttattg ttgcccggcg tatggagtaa atatcacgtc agctggtaat
     9661 gacgatcggg aagaattttt gctggaattt cggcttcatc gttcatctgt aacaggtcaa
     9721 tttcaatagc gttgcagatg gcatccagtg gtaaatcatt gttttcagta ccgaacggat
     9781 cttccagttc ttccgccaga caatccagcg aaataaaagt gtaggaaatc agcacagaga
     9841 taaaaggcgt catgtaatgc aggtccacga ccagcgcgaa cggcagcatg atacaaaaca
     9901 gataaacggt acgatgcaaa atcagggtgt aggcaaaggg aattggcgta taggcaatgc
     9961 gctcgcatcc tgccaggacc gctgaaatat cattaagccg atcgttgagg ctaataaaca
    10021 ggatatctga aagctgtcca ttgcggcgct gaaccgccaa ccattctccc attattaaca
    10081 agatacggtt agctggagag ttcgaagcca gtacacgctg aagatcttca gtcttgagat
    10141 aatgagccag cacttccgcc tgtggctgtt tgcgtaatgt catgcgtaaa cagtgggcga
    10201 aagcgatttg cagccgggca aactccctta cacttgccga atccggcaat gtcgttttta
    10261 cctcgcgcag taacgaccgt gaggcaatca tcaactgccc ccaaagtttt cgcgcttcaa
    10321 cgtaacgggc gtacccggca ttattacgaa aaccaagaaa aatggcgatg gcgacaccga
    10381 gaatgctgaa cggtgcgagg gtgaatttga tgcccagatg cgtgtaccag ggcagcatga
    10441 aaataacagc gataaaaaag agaaaattga gtagtaagcg cgaggatatc ttggataata
    10501 ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga acaatcatgg
    10561 ttatcttcag gcgtggaaaa gtcgccctat taaacgtgat tacgatcaca ttctcaagac
    10621 gctacttaca aattacctac aagcttacaa cattagcagg cgctgcatgt ggcacctgtt
    10681 aatgataaag gcatatagga tgttgtaact aatatggtga aataagaaac ccggtcgaaa
    10741 ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc gcatcccttt
    10801 ttcgagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca cttgcgtcag
    10861 gtcctgctcc cagtggtctt tttctgccaa aacaatcgct accagttcct gggtgccgat
    10921 aacacggtca cgatgctggc tccagagttg ctgataacgt tccagccagt gtgcatcgtc
    10981 ctgtaccgga tatgtttcgc cgttacgctc accacgatag aacgcaatca atgccgctaa
    11041 tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca gcagctgtgg
    11101 caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca gctgatgctt
    11161 aatgtacggg ttacggaaac gcccggtgac tgcactggcg aaagattcca gttcatcacg
    11221 aggcaaatcc agtaccggaa taatttcttc gtagatagct ttttcaacga atgcgcaaat
    11281 ttcagcatcg ttcatcgctt cacctacggt atccagccct gcctggaacg ccaccggcac
    11341 cagcgcggtg tgcgcaccgt tgaggattgc cactttgcgt tctttgtacg gtttaatatc
    11401 gtcaacaatc agcacgttga gcggatattt gtccagacgc agttcagtcg ctaaggattt
    11461 cggtccctga atcacaaaca ggtaaaagtg ttcagcggtg tcgagaaaac catcgtgata
    11521 acccagttct tcttccagtt tagccacttc atcgcgcgga taaccggtaa cgatacggtc
    11581 caccagcgta gaacagaagc tgttagcctg atccagccat tgaataaatg cttctggcag
    11641 tgcccactct tgcgcatagc gcagcaccag ttcacgcaac gcgtcgccat tgtagtcaat
    11701 caactcacac ggaatgatga tccaaccttt atccagcgca ccgttgaaat ggctaaagcg
    11761 ttcgaacagc agacgggtca gttttgccgg atagcttacc gctggcgcgt catcgaattt
    11821 atcgcccgca tggtagctga tacccgcttc ggtggtgtta gagaaaacaa agcgcatttc
    11881 cgggttgtga gccagtttca ggaattcatc gtattcactg tagacgctga tttcacgatt
    11941 aaccgagcga atcagacgcg cgtcgctgac cgcttccccc ttctcattca ggccacggat
    12001 aatggtggtg tacagaccat cctgcgtgct cagtgacggc gggaatgaag tttcaatcgg
    12061 acgaacaacg accacgccag aattcagatc ggtgtgctca ttcaggagat cgatttgcca
    12121 gtcaacaaag gcgcgcagga agttaccttc accaaactga atgatacgtt ctggatactg
    12181 tgcaccggga aaatcgcgac gatttagtgt tttcacaatg ggttcccttc tgattagtca
    12241 tacaacctgt ttgaattggt acgacaggtt agcaaacttt aatacgccga acccctgttt
    12301 tgatcaactc ctgatgatta atgagcagtt ttatgagaaa agtgtggcgc ggatcatggt
    12361 ttaatcgagg aaaaacgcct tttcctggat cataaagtgg tagaacacat tgcattcaaa
    12421 tcgcgcgtaa tgaataaaga tgtcagacaa cttcctcacc gtaacgcata gtgctggtac
    12481 ggttgcgccc atctttcttc gagcgataca gacacgtatc ggcttcgacc atcaatttat
    12541 taaagtcatc ggtcagcgtt cggtacgatg cgcgaccact accgacgcca atacttaccg
    12601 tgagataaag cgttttttgt tgccaggtga atggttgcag ttcaacgcct ttacggattt
    12661 tttccgccat tagcagacca tcgacaggat tcaccgacgg cactgcaaca gcaaattctt
    12721 cgccgcccat tcgcgccacc agccccttat caccgacaat cttctgaata tgccgggcaa
    12781 acacgcttaa cactttatcg ccacattcat gtccatagtt atcgttaatg cttttgaaat
    12841 agtcgatatc aagcagcatg accgtcagat gttgtgtctg tttcagcgat ggacttttca
    12901 gcgcctcata aagaccggac cgtgagtaaa cctgagtcag aaaatcaaag tcggctcgca
    12961 gcgcaacttg cttcattagc gaattgatcg ctgccacgct aaaagaaacc ataattgggc
    13021 atatcgccat cgtggcaata ccgagacgtg cggagaacat ttccggaatg gagaacggcg
    13081 aaccgaccga gatatcaatc accgaatttg ccaccagtac gatttccacc gcaccggtga
    13141 caaaggtcaa cagacatgtt acctgcggcg tatagcgcac tgcacaccag attaatgcag
    13201 gcagcgggaa cgccagactc cccgctccac caatgactac cgaggcaata accgacacaa
    13261 taagcgcgat ggcgggcatc atctgttctg ctttaaagcg cggcagcact ccaggaatag
    13321 ccaacgtcag catacaaggc acgatcaaca cgcccgttga gaattgctca ctgaaccagt
    13381 cggcaagcaa aggccagaaa tccagactat caatactgac cgaaccaatc gccccgacaa
    13441 tagcgcataa taatgcggca atcagacagt aattaaatag ccgtaaggca cttactggct
    13501 catacttatt tttccccaga cgcttatccc gcacgaccag taaggcgacg gtaacaataa
    13561 acatcatatt ggagaaattg atagccagtg aaaccagtcc ccattcggtg gttatggcat
    13621 cataaaccag catcgccaca taactgatcg catagtaatg cagtcgatta agccagacat
    13681 agcgggcaaa cacccccgcc attacgccat tcagcggcca gaaaagagaa agttcgtgaa
    13741 ccagtcgtaa ctctgcacca ataaaataga acagcgtggt tagcgcaaaa atagcgatgc
    13801 tattacgtag cagatgacct tcctggaaca aacggaaagt tgaaatgggg tgtacatgca
    13861 ttaattatct gtccatacaa ttgcgggtgg caattatctc aacgtaataa gtaaattctc
    13921 ataaaggata gcatgttcgc taacagcggc ggcaaataaa ggtaccctgg gaatgctatt
    13981 ttatcgtttt ccgcaattct catggctgat tcatctgtac aaccagccag tgatagatta
    14041 ccgccacgat ataacgttgt tgttcttcgc ttaacgaaac accctgcgga atgttatgcc
    14101 acttagccag acaaccgcga cagcaggtcg cggtggcgtg ctgagcgata aacaccggat
    14161 gcccgcgcat gggggtttgc ttaccgtcat tagccggtaa tgccggagca agacgtttag
    14221 cgacaaaatc cgccgcatgt tgttcgatga ctggcgcgcc tttctccagg caatactgac
    14281 gctctttcat gcccagacga aagcgagagc gaaattttga acgggataaa cgcgcgaaga
    14341 gtggatcgag agactgcatt aataaaccga acgccctaac tgtttcgtca attgttcaag
    14401 gacggcaata cccgcaagcg agttacctgc atcatccagt tccggactcc agacagcgat
    14461 ggccatttca tgcggaacaa tcgccacaat accgccacca acgccagatt tcgccggtag
    14521 ccccacccgc caggcaaact cccccgcgtt ctggtacata ccactggtcg ccatcagcgc
    14581 gttaatttgc cgcgcctgca ttggcgtcac cactggttca tcaatatgaa tagctttccc
    14641 ctgattagcc agaaagacaa acgtccgggc cagctctaca cagctcattt tcagagcgca
    14701 gtaatgaaag tagttttgca gaacggttgt cacgtcatga tggaaattgc caaacgactt
    14761 catcagccag gcgatagccg catttcgcgc ggaatgttca aattcggaac gcgctaccac
    14821 cgtatcgtag gaaatatcag acacaccgct taagccgcgc acgacttcca gcatacgttg
    14881 ccgtggtgcg cttaatcgcc cttgcaacat atcgcagacc accagcgcac cggcattaat
    14941 gaacggatta cgcggtatac cctgctccat ttccagttgc actaaggaat tgaacggtga
    15001 tccagacgga tctttgccga cgcgttgcca gatttcctct tcggagtaat gacgcatggc
    15061 gacaacgaga ctcagcactt tggaaataga ctgaatggaa aaacgttctt gcgcgtctcc
    15121 ggcctgaaaa agctgtccgt caacggtaca gatagcaatc cccaatcggg aaccgtctac
    15181 tgtagccagc gccggaatat aatccgcgac tttaccctga ccaatgagcg gccgcacttg
    15241 ccgcaagatg ttttctaaaa ttgcattatc catggcgact gccactttct actcctggac
    15301 cgcaggtctg aaaagacctg cgagtatatc agagctgaat atgtcgcgtc agatccggtc
    15361 tttccacacc gtctggatat tacagaattc gtgtaagccg aaatgggaaa gctcacgacc
    15421 aaagccactc tttttcacgc caccaaaggc cactcgcgcg tcgctggcac aataaccatt
    15481 gataaacacc ccaccgcatt ccagacgtgc cgccatctgt ctggcctgtg tttcgtcggt
    15541 ggtaaaaatg gtcgctgaaa ggccgaactc actatcatta gccagttcca gcgcatgttc
    15601 tgcatctttc gcaacggtga ttgccgcaac ggggccaaac atttcttccc gaaacgcggt
    15661 catttctggg gtaacattcg ccagaaccgt tggcggatag tagttacctg ccccagccat
    15721 cttttccccg cccagtaaca aacgcgcacc ctgcgccagg gttttctcca cctgatgatg
    15781 cagctcatca cgtaaatcaa aacgagccat tggtccgaga gcgttctctt catcacgggg
    15841 atcgcccatt ttcaaggctg ccgcagctgc cacaaaacgt tcggtaaatg cagaagcaat
    15901 tccctcttcg ataataaagc gttttgccgc tgcacatacc tgtccggtat tctgataacg
    15961 tccggctacc gccgctttca ccgccagttc cagatcggca ttgttaagca caataaacgg
    16021 atccgaaccg cccagttcca gtacgcattt tttcagtgcc gctccagcct gtgcgccaat
    16081 agccgctcca gcacgaacac ttccggtcac cgtgacagca gcaatgcgcg agtctttaat
    16141 catctgactg acaccgtcgt tgtcggcatt cagccagcca tatacgcctt gcgggatccc
    16201 cgcatcttta aacacctggg caatgagctg agcacagccc atcacattcg gcgcatgttt
    16261 aagtaagtag ccgttacctg caagaatgat gggaaccgcg ccacgcatca cctgccataa
    16321 cggaaaattc cacggcataa tcgccagaat cgtccccaac ggtcgatact caataaccgc
    16381 ctgctgattt tccaccagcg taggttccgc cttcagcatt gccggaccat gttctgcata
    16441 ccagtcacac aaattcgccg atttcgccac ttcagcgcgc gcctggttga ttggtttgcc
    16501 catttcgcgg gtgatcattt gcgccatttc ttcgttacgg gcgagcagag ccttaccgat
    16561 atcacgcagt ttttcggcac gataatctat atttgtctca cgccagtcgc gaaagccagc
    16621 tgccgccagc tgaagtgcgt tttcgatatc gtcagcgcca gcccacggca gcacagaaag
    16681 ttgttcaccg ttggcaggat ttatcgaaat tgcatgagtt gccggagtaa tggtcatcgg
    16741 ggtatctcct ttatgagtca tggtatgaag atacgcagat ttactcttgc tttaaaatga
    16801 ataatattaa gccacttatt cacgaatcga gaatattatg gatctgaccc aactggagat
    16861 gttcaacgcg gttgccgaag ctggcagcat aacccaggct gcagcaaaag tgcatcgcgt
    16921 gccgtcgaat ctcactaccc gtctacgcca gctggaaaca gaactggggg ttgatctgtt
    16981 tattcgcgag aatcagcgtt tacgtctctc tcctgccggg cataactttt tacgctatag
    17041 ccaacaaatt ctcgcgttag tggatgaagc gcggagcgtt gtcgctggcg atgagccgca
    17101 aggtttattt tctcttggtt cgctggaaag caccgcagca gtgcgcattc cggccacgct
    17161 ggcggaattc aaccgtcgtt atcccaaaat tcagttttca ctttccaccg gcccttccgg
    17221 cactatgctg gaaggggtgc tggagggaaa actgaatgcg gcatttattg atgggcccat
    17281 taaccatact gccattgacg ggataccggt gtaccgcgag gagttgatga ttgtcacgcc
    17341 acaaggacat gcgccagtaa cccgtgccag tcaggttaat ggcagtaaca tttatgcctt
    17401 ccgcgccaat tgttcgtatc gtcgccactt cgagagctgg tttcatgctg acggtgccgc
    17461 tccgggaact attcacgaga tggagtctta tcacggaatg ttggcctgtg tggtcgcagg
    17521 agcaggcatt gcgcttattc cacgctctat gctggaaagt atgccggggc atcaccaggt
    17581 tgaagcgtgg ccgttagctg agcaatggcg ttggttaaca acatggctgg tctggcgtcg
    17641 tggtgcaaaa acgcgtccgc ttgaggcatt tattcaacta ctggatgtgt ctgactcggc
    17701 aaaacagagt tagcaatgag ctatttttga tagttcttgc ggctaatatg ctctatatag
    17761 tgatgttccg atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatcagc
    17821 aattacatat ctcttcctga tgattttccc gttcgtaata ttgcaccaca ggtaaaagaa
    17881 gttttaaagg attttattga tgcactcagt actataatat gtgatgaaga gtggcgcacg
    17941 tctttaaaca ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat
    18001 attcagagga cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa
    18061 ctcacttatc ccgccaggaa tggaagacac aaagaaaata ttgaatttca ggtagtaata
    18121 aatttaagcc ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc
    18181 gctcccaacc caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgta
    18241 ctctttctgt ttctgaatga ttttattgaa caatttccaa tgatcaaccc tggtgttccc
    18301 atcaaaagag cgcatacacc acatattgaa cccctgcctt cggatcacca taccgctgca
    18361 gattacttac gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc
    18421 atactccccc gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct
    18481 aacttgatca ccgccattct cgacataacg gacagcgaat taaaagagga tttgttgcaa
    18541 agcctgatgg attcattagg ttctaacaaa catgtactac ccgaagtctg catctgcttt
    18601 ttatcccttt tagcagaaca agaatctcct cattttcaga acttgttttt atttttcgcc
    18661 aatatgttat tgcactatca ccaatttatg aatcccaatg aaagtgattt gaatgacgtg
    18721 ttgatgccag catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc
    18781 aaactgtttg taaaaaatga aacaccgcca aaagttactc acgaagacct ggtgaaaaac
    18841 aggcctcgct cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct
    18901 gaacgtcatt aaaccgtgat gttaccgact ctctgacgcg tgaaagaatc agcgtcagag
    18961 aaacggaaaa cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag
    19021 atcatttcat cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt
    19081 cccgcaaagt ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca
    19141 ccaccgaatt tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa
    19201 ccgctcaggt cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc
    19261 cttttatgtt aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg
    19321 tgtttattgc cagccacgta ctgtcgtttt tgtcgtggag ctttaccgtt ctggtgatca
    19381 gtcgcattgg tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga
    19441 tccgtatggc tccggccggg aagcgagcac aggcattgag tttaattgcc accggtacag
    19501 cactggcgat ggtcttaggt ttacctctcg ggcgcattgt gggccagtat ttcggttggc
    19561 gaatgacctt cttcgcgatt ggtattgggg cgcttatcac ccttttgtgc ctgattaagt
    19621 tacttccctt actgcccagt gagcattccg gttcactgaa aagcctcccg ctattgttcc
    19681 gccgcccggc attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca
    19741 cggcatacag ctatatcgag ccttttgtac aaaacattgc gggattcagc gccaactttg
    19801 ccacggcatt actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta
    19861 aactgggtaa tcagtatgcg tctgcgttgg tgagtacggc gattgcgctg ttgctggtgt
    19921 gcctggcatt gctgttacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt
    19981 tctgggggat cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg
    20041 caccagatgc taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg
    20101 gggcgggtgc gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt
    20161 atgtgggcgc ggtgcctgct tttgccgcgt taatttggtc aatcattata tttcgccgct
    20221 ggccagtgac actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa
    20281 tggtacgttt taatgatttc caggatgccg ttaataataa actgcacacc catacatacc
    20341 agcaggaatc ccatcagacg ggagatcgct tcaatgccac ccttgcccac cagccgcata
    20401 attgcgccgg agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc
    20461 ggcggcgcaa ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc
    20521 gagctaataa tcatcgctat ggttcccgga ccggcagtac ttggcattgc cagcggcaca
    20581 aaggcaatat tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt
    20641 gaatcaatcg ctttctgttg cggaaagagc atccgaaaac cgataaacgc gacgattaag
    20701 ccgcctgcaa ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgcccg
    20761 gcgtaatacg ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga
    20821 ttacgttcgg cactgttcat gttgcctgcc aggccaagaa ataacgcgac agttgttaat
    20881 gggttagcta acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac
    20941 attggtggtt gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag
    21001 gataaagtgt cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta
    21061 gcaaaacgtg gcatcggtga attcattcat ttgacttata cttgcctggg caatattatc
    21121 ctctgcaact aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat
    21181 gaaattattc cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt
    21241 aacgagtatc tgtctccgct ggatattact gcggcacagt ttaaggtgct ctgctctatc
    21301 cgctgcgcgg cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga
    21361 gcactgaccc gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac
    21421 ccgaatgata agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa
    21481 caatgccatc aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg
    21541 gacgaagtgg caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg
    21601 tatgacgatg tccagacgca atactgacgc tattaccatt catagcattt tggactggat
    21661 cgaggacaac ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt caggttactc
    21721 taaatggcac ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat
    21781 ccgcagccgt aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact
    21841 ctatctggca gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa
    21901 ttactttgat gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt
    21961 tttacatcca ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa
    22021 tcatgaaacc acttttatcc gcaatagcag ctgcgcttat tctcatttcc gcgcagggcg
    22081 ttgcggaaca aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc
    22141 catcgcagga acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg
    22201 atgcgctcgg cgtgccctat tataatcaac acgctatgta gttattgtca ggctctgatc
    22261 acagagcctg acatctccgt aatatcttcc tgaagtctca aatattttcg ccattgttgg
    22321 cgatgagatc ccgataccag taaaacgacg ctttacgtat ccgttccagc gttcccttcc
    22381 cttcgttgtc tttatcgaca taaatcattc cgtagcgctt tttcatttcc cctgttccag
    22441 cagaaacaag gtcaatgcag ccccacgggg tgtagccaat taagtcaaca ccatcttcga
    22501 ctacggcttt tttcatttcc cgaatatggg aagcaaagta atcaatgcga tagtgatcgt
    22561 tcaccgtgcc gtcagcttgt ctctggtcaa ccgcaccaaa tccattttcg acaataaaca
    22621 gcggcaattg aaaatgatcc cagaaccagt tgagtgaata acgtagccct gccggatcaa
    22681 tttgccatcc ccattcggat gtatcgatat aagggttgct gaccaaatct cgtggttcaa
    22741 cataatccag ttgcgggtta tcgggcgaaa attgtgtcgt aaaagacatg tagtagctaa
    22801 aaccgataaa gtcgacacat ccactggcaa gaatcgcgtt atcttctggt gtgatatcga
    22861 ggttgaatcc tttcctggca aagtaattca gcatatgttg cggataatat ccacgagcat
    22921 gaacatcagt aaaccagtaa cgacgatgca tcgctttcgt ggccatcatc atatcgttgg
    22981 gtgcacacgt cagaggatag atgggacaca tggcgatcat acagccaatg ttaaattcag
    23041 gattgatcga ttttccagtc tgtaccgcca gggcactggc aactaactcg taatgtaccg
    23101 cctggtacat tatttgttcg cgctcattga tatcttcctc tggcgaatac aagataccgg
    23161 aattagtaaa tggacacagg ctttcgctga aattcacctg attattgatt tcgttaaacg
    23221 tcatccagta ctttactttt tctttatagc gcgtgaagac cgttgatgcg aagcggataa
    23281 aaaagtcgat cagtttacgg tttcgccagc caccatattt tgtcaccaga tgataaggca
    23341 tctcaaaatg tgaaagcgtc accacaggtt ccattccctg cttaaggcat tcatcgaaca
    23401 gatcatcata aaatcgtaaa ccctcttcat tcggctcctg ttcgtcacct tgcggaaaga
    23461 ttcgtgtcca ggcaatggaa gttcgaaagc atttgaatcc catctcggca aataactgaa
    23521 tatctgtttt atagcgatga taaaaatcaa ttgcttcatg attgggataa ttaagcccgt
    23581 cgataacgcc ttctgtcact tcacgcggta ccccgtgagc gccagcagtc atcacatcag
    23641 cgatactgat gccttttcct ccttcactcc agccaccttc caactgatgc gcggctaccg
    23701 cgccgcccca taaaaaacct tttttaaatc ctgacataca caactcctta actaaaatag
    23761 attaccacca gacttccgcc tggatgccgg ccataaactc gtctttgctg ttctcattaa
    23821 acttaaaatt cgataattcg ttatccagaa tattgatata cgttccatag aagcgaattt
    23881 ctggtcgtga acctaaaatg ctttcaccca ctttcaatgc atgccagagt gtcgttttat
    23941 aagccgattc atttagtgtc acaccctgct gagttttgtt ctgttgctta aaccagccta
    24001 attcaagccc cgtctgattc catgtattcc agatccaggc cggtcttatt acagtgcgaa
    24061 tactgtcaaa atcactatga gcgccacttt cataactata aacatcttcg ccgtgagaat
    24121 agacaagcgc gttagccata ataatattgt cagtaagata catctcgccc tgagagatta
    24181 aacgccaggc gatcccattg gtatggtcac catagtaata gcgaccgtgc gccatcgtat
    24241 tactggcatc tgagaaactg gcaaaactgc tggcatagga attattggca acctgtaacg
    24301 taaattcatt aaacgtatcg cgttgcaggt tttgccgtaa aacagaagtc agcatccagc
    24361 tatctttcat ttcaaaataa ctgtcgtcat tttcgttatc ttgttgttca tccgttttat
    24421 taggtgcgga atatttagcc attaatgata atgtcgcatc atcccataac gggatattgc
    24481 gataacgaac atccacagaa ttggtattca tctggctggt acgcgaaaaa tcacgagagt
    24541 aaacatcgac atcatcacgg cttaaggaca tatcaaacag ccctacacca agtgcccagt
    24601 tttcaatccc cacacccgcg gcgacatccg tggttaaggt tttccagtcc agcatctgga
    24661 tctcatattg cgggagttta tgtttgccga cccagaagtc tgcctctggt gcgaagggta
    24721 aaaaacctcg cgttgtcatg taaatatcgc tgaactgcat gatattttca ttggcactgt
    24781 caccaaacca ggcatcgtta tactgctcac ccacgttgcc atcataggta acgaccgcat
    24841 ttgccgtttt accgtcctga ttataaacac gctgatttaa ggtcaggtca aaccaaccgc
    24901 tcatctcatt accaaaacgt cccagagaac ccgctgcata agtttgtggt gagccgtgat
    24961 taccagtacc ccagcctgag cggaagtacc cctgatagct gaacccaata tcatccttta
    25021 tatatttact gatatctttc agggtcacgc tttcgatagt ggttttgtcg cctttacttt
    25081 caataacggc aacttgctgc gatccattta tagtatgcgc tgcggtaacc gagttctgtt
    25141 caccgttttc atttttgatg ttatcagcga cgggagatgt cgctgatatt tcagcaagcg
    25201 aggctgattt atatttgttt tccgtgatgc ttttttgtaa ggtcgaaagt cgggatttat
    25261 atactcccag ttcattctgt gttgctttca gctcttgttt attttgcgac aattcatttt
    25321 caagcaattc aaggcgctgc tctatggtta attttgttgc catcgcgttc aatggaaaca
    25381 atgctgtaat aatagaaaaa gacaaaaggc tgacgttaag cgtctttata ttcatgactt
    25441 atccttaaga gagtaataat ttcctccagc tatcgcactg gaggaaaaaa tcaggcatgg
    25501 agttcttttc ttaatgaaat aatttccctg gctaaatcct ggcataatat tgcattcatc
    25561 aaatgatcct gtgcatgaac cagaattaaa ttaactttta ttttgccaca gccttcatcc
    25621 tgactaatta atgcggtctg aatcttatgt gcctgaagac aggcagattc agaactggcc
    25681 attagctgag ttgcgccctg ccagtctttt tgcccggcac aacgtatggc ttccatcgcg
    25741 tcagaacgtg cctgccccgc gttgaccagt aactccataa caagttcttc atctgcaaac
    25801 atcacaatct ccttcctggg taaatggaat tatccgttag ttgtcatgtt attgactttg
    25861 ttggcggcaa taacaaaagg taaatagatc attgtcgcca cacctaaaca taaaaaacca
    25921 accagtaaag ccagccagtt tcccccggtt ccgaggaaag caattaaagg tccgggcgta
    25981 gtccagggca cggcataagc aaccggtgga ataatttcca tcgaaacaaa gaagtaacca
    26041 atggcacagt taacaatggg aaccataata aacggcacca tcatgatggg gttaagtaca
    26101 ataggaaggc caaacattat cggttcattg atattgaaaa taccggggat aaatgacatt
    26161 tttgccagat cacggtattc cgcacgctta gaagcgataa aaatagccaa caataacccc
    26221 aacgtcatac ctgaaccgcc gcagttggcg aatgcatcat tgatagaagt ccaggtaatc
    26281 ggatatggcg cgccccaggt agtgccgtgt tgagcggccc aggagagatt ctccagatta
    26341 gcctcagaaa aaacagtttc tcgaattgcc gacgtggtat taggtccgtg gatccctagc
    26401 acccacagga aattacccac aactcccagg ataattaccg caaagatatt cgttcccata
    26461 tgtttgagcg gcgtctggat taatgtgtaa ataagatcgt ttaatcctgc cggggatatc
    26521 agtgtcaggc aataattaag tgcggaaaag aacaccatga caaaaaatat tggcaataaa
    26581 actttaaatg aacgcgctac cgcaggcggt acagctgccg gcatcgttat ggtgattttt
    26641 gggtttcgag ctaagcgaca aaatatttca ctggtgatca atgcaacgat aacagcaaca
    26701 aacaacccct gcgcgccgag atatttggtc gtcagaaatg cttgcccatc tatcatctga
    26761 tatggtgtat aaacaataaa aaaggctcct attgccgtta atccgcataa aagatcatct
    26821 tgctcatagc taatcgccat attcctggcg accaggaaag caatcataat tgacatgata
    26881 tttacagaac cattcattac cggagtaaaa atagattgtg ctttttcaag gttggggaaa
    26941 atgctgttca gatgcagtaa tccggcaata aaaccgtctg gcgataatat ggcaaagtta
    27001 attaatataa ttaatgaact tgccatgata attggaaacg taaaaacgaa tccatctctc
    27061 accgctgcaa catgtttttg tgagtttaac ttgatagcaa ctggaacaag aaaacgttcc
    27121 attccacgtt cgaatgaggc cattaatccc atttttttat acctgctgtt ctggatattt
    27181 atacatccca ataaagtatg tatggcatat ccattttatg atgtgtgaaa agttagttca
    27241 ggtaatagtt taataatatt agattcttac tgttgcagta gtttcatcgc aaattcaaga
    27301 actgcctgtc cgttcatagt gccatagtct ttcatctcga taacggctac aggcattttc
    27361 ccctgtgcaa cggcttcaat ctccggtttc tgaaagcgaa cttgcgggcc aagtaaaaca
    27421 acgtcaactt cgttattttt aattttccct ttagcctctg caatcgccaa tgcggaaata
    27481 ttaacttcaa gtgaaatagc gttagcatga tcaatcatac gtttaaccag catactggtt
    27541 gacatgcccg cagcgcaaac taacaatatc tttttcatat tttcccttac tggtatataa
    27601 cagactacat tgtgtaaata tcatatcccg cagtagctat acatcctggt acattgatca
    27661 cagtaataca gtaataaagt tgttcaacat cagggtgata attaaacgac tcggccatac
    27721 gataaaccaa tcaggactca accccttgtt ttcaatggct cttaaaaggt gcaaaagtga
    27781 tttttcaaaa gattgcccgc ttacttaaat ccgagatcaa tggctattca tggcatgttg
    27841 gtgatttgtt gccgtcagaa gctgaactcg ctgttcgtta taatgtttcg cgtaataccc
    27901 tgcgtaaggc attatccttg ctggaaggcg aaggaattat tcacagaaag catggttcag
    27961 gaacatacat taaaaaaaag aattttgttg cacacattga tcacatgaac agtttcagtg
    28021 aaattgcgca taaaagcggc aaagaggcag ggagtcagat tatgaaattt gaagtgcagg
    28081 atgcttcacc cactattgcg actgagctga atttagtgac tggtgagcag gtttattaca
    28141 taaagagact gcgatttatt gaggataatg cagcacaatt agaagaaacg tggatgtcag
    28201 tggcacgttt tcctgattta accgtagcac atatgcaaaa atccaaattt tcgtatattg
    28261 agaacgaatg cgggatcaaa atcattggca cctttgaaac tttctcccct acttttccta
    28321 ccccagaaat cgccagtatt ttacggatca gcccacggga tcccatactt aaaattcaga
    28381 cccaggctgt ggacagtaac tctattccgc tggattattc gttactttac agcaatattt
    28441 tcgagttcca ggtaaagtac tttttcccgc gataaaagca gacgcacaga gtgctgacat
    28501 cacattctgt gcgtatggtt tagcctctaa cctttaccgc tttacgccac cgcaagccaa
    28561 atacattgat atacagcccg gtcataatga gcaccgcacc taaaaattgc agacccgtta
    28621 agcgttcatc caacaatagt gccgcacttg ccagtcctac tacgggcacc agtaacgata
    28681 acggtgcaac ccgccaggtt tcatagcgtc ccagtaacgt cccccagatc ccataaccaa
    28741 caattgtcgc cacaaacgcc agatacatca gagacaagat ggtggtcata tcgatagtaa
    28801 ccagactgtg aatcatggtt gcggaaccat cgagaatcag cgaggcaaca aagaagggaa
    28861 tgattgggat taaagcgctc cagattacca gcgacatcac cgccggacgc gttgagtgcg
    28921 acatgatctt tttattgaag atgttgccac acgcccaact aaatgctgcc gccagggtca
    28981 acataaagcc gagcatcgcc acatgctgac cgttcagact atcttcgatt aacaccagta
    29041 cgccaaaaat cgctaaggcg atccccgcca attgtttgcc atgcagtcgc tccccgaaag
    29101 taaacgcgcc aagcacgata gtaaaaaacg cctgtgcctg taacaccagc gaagccagtc
    29161 cagcaggcat accaaagtta atggcgcaga acagaaaggc aaactgcgca aaactgatgg
    29221 ttaatccata ccccagcagc aaattcagtg gtactttcgg tcgtgcgaca aaaaagatgg
    29281 ccggaaaagc gaccagcata aagcgcaaac cggccagcat cagcggtggc atgttatgaa
    29341 gccccacttt gatgaccaca aaatttagcc cccatacgac cactaccagt agcgccaaca
    29401 ccccatcttt tcgcgacatt ctaccgcctc tgaatttcat cttttgtaag caatcaactt
    29461 agctgaattt acttttcttt aacagttgat tcgttagtcg ccggttacga cggcattaat
    29521 gcgcaaataa gtcgctatac ttcggatttt tgccatgcta tttctttaca tctctaaaac
    29581 aaaacataac gaaacgcact accggacaga caaatgaact tatccctacg acgctctacc
    29641 agcgcccttc ttgcctcgtc gttgttatta accatcggac gcggcgctac gctgccgttt
    29701 atgaccattt acttgagtcg ccagtacaac ctgagtgtcg atctaatcgg ttatgcgatg
    29761 acaattgcgc tcactattgg cgtcgttttt agcctcggtt ttggtatcct ggcggataag
    29821 ttcgacaaga aacgctatat gttactggca attaccgcct tcgccagcgg ttttattgcc
    29881 attcctttag tgaataacgt aacgctggtg gtgctctttt ttgcccttat taactgcgcc
    29941 tattccgttt ttgctaccgt gctgaaagcc tggtttgccg acaatctttc gtccaccagc
    30001 aaaacgaaaa tcttctcaat caactacacc atgctcaaca ttggctggac catcggtccg
    30061 ccgctcggca cgctgttggt gatgcagagc atcaatctgc ccttttggct ggcagctatc
    30121 tgttccgcgt ttcccatgct tttcattcaa atttgggtaa agcgcagcga gaaaatcatc
    30181 gccacggaaa caggcagtgt ctggtcgccg aaagttttat tacaagataa agcactgttg
    30241 tggtttacct gctctggctt tctggcttct tttgttagcg gtgcatttgc atcatgcatt
    30301 tcacaatatg tgatggtgat tgctgatggg gattttgccg aaaaggtggt cgcggttgtt
    30361 cttccggtga atgctgccat ggtggttacg ttgcaatatt ccgtgggccg ccgacttaac
    30421 ccggctaaca tccgcgcgct gatgacagca ggcaccctct gtttcgtcat cggtctggtc
    30481 ggttttattt tttccggcaa cagcctgcta ttgtggggta tgtcagctgc ggtatttact
    30541 gtcggtgaaa tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca
    30601 ggaatgaaag ccagctattt ttccgcccag tctttaggct ggcttggtgc cgcgattaac
    30661 ccattagtga gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt tgtcatctta
    30721 gcgttggtga tcattgctgc gtgggtgctg atgttaaaag ggattcgtgc aagaccgtgg
    30781 gggcagcccg cgctttgttg atttaagtcg aatacaataa aaatttaatt cagccttcgt
    30841 ttaggttacc tctgctaata tctttctcat tgagatgaaa attaaggtaa gcgaggaaac
    30901 acaccacacc ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta
    30961 ctgggaataa ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac
    31021 taatgaacgg agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc
    31081 ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaaag cttgcatgga
    31141 tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg
    31201 acacagtagc atcagttttc tcaatgaatg ttaaacggag cttaaactcg gttaatcaca
    31261 ttttgttcgt caataaacat gcagcgattt cttccggttt gcttaccctc atacattgcc
    31321 aggtctgctc ttccaatgac cacatccaga ggctcttcag gaaatgcgcg actgacacct
    31381 gctgtcacgg taatgttgat atgcccttca gaatgtgtga tggcatggtt atcgactaac
    31441 tggcaaattc tgacacctgc acgacatgct tcttcatcat tagccgcttt gacaataatg
    31501 ataaattctt cgcccccgta gcgataaacc gtttcgtaat cacgcgtcca actggctaag
    31561 taagttgcca gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc
    31621 aatttaaatc ggtcaatatc caacaacatt aaataaagat tcagaggctc agcgttgcgt
    31681 aactgatgat caaaggattc atcaagaacc cgacgacccg gcaatcccgt caaaacatcc
    31741 atattgctac ggatcgtcag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa
    31801 agcaacccct cctgaaaggc gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc
    31861 agcattaatt cccgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg
    31921 taaggtaatt catcgttatc gagtggcccc agatgatcaa tccaccgacc aaactggcac
    31981 agtccataag aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg
    32041 tgaaacatac tcaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag
    32101 atggcatcaa tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg
    32161 cgcgctattc taacgagaga aaagcaaaat tacgtcaata ttttcataga aatccgaagt
    32221 tatgagtcat ctctgagata acattgtgat ttaaaacaaa atcagcggat aaaaaagtgt
    32281 ttaattctgt aaattacctc tgcattatcg taaataaaag gatgacaaat agcataaccc
    32341 aataccctaa tggcccagta gttcaggcca tcaggctaat ttatttttat ttctgcaaat
    32401 gagtgacccg aacgacggcc ggcgcgcttt tcttatccag actgccgcta atgttgatca
    32461 tctggtccgg ctgaacttct cgtccatcaa aaacggccgc aggaataaca acattaattt
    32521 caccgctctt atcgcgaaaa acgtaatggt cctctccttt gtgagaaatc aaattaccgc
    32581 gtagtgaaac cgaagcgcca tcatgcatgg tttttgcgaa atcaacggtc attttttttg
    32641 catcatcggt tccgcgatag ccatcttcta ttgcatgagg cggcggtggc gctgcatcct
    32701 gttttaaacc gccctggtca tctgccaacg cataaggcat gacaagaaaa cttgctaata
    32761 caatggcctg aaatttcata ctgactcctt aattgcgttt ggtttgactt attaagtctg
    32821 gttgctattt ttataattgc caaataagaa tactaccaat tgttataagg catttaaaat
    32881 cagccaacta gctgtcaaat atacagagaa tttaactcac taaagttaag aagattgaaa
    32941 agtcttaaac atattttcag aat
//
LOCUS       DAZFZC010000048        32559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFZC010000048 DAZFZC010000000
VERSION     DAZFZC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.048"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(105..1295)
                     /locus_tag="ACHKXP_002648"
     CDS             complement(105..1295)
                     /locus_tag="ACHKXP_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7647626.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
     gene            complement(1472..1547)
                     /locus_tag="ACHKXP_002649"
     tRNA            complement(1472..1547)
                     /locus_tag="ACHKXP_002649"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1512..1514),aa:Phe,seq:gaa)
     gene            complement(1653..2360)
                     /gene="yqgA"
                     /locus_tag="ACHKXP_002650"
     CDS             complement(1653..2360)
                     /gene="yqgA"
                     /locus_tag="ACHKXP_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO7647627.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..2596
                     /gene="yqgH"
                     /locus_tag="ACHKXP_002651"
     CDS             2513..2596
                     /gene="yqgH"
                     /locus_tag="ACHKXP_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO7647628.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2758..4893
                     /gene="speC"
                     /locus_tag="ACHKXP_002652"
     CDS             2758..4893
                     /gene="speC"
                     /locus_tag="ACHKXP_002652"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO7647629.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4943..6199)
                     /gene="nupG"
                     /locus_tag="ACHKXP_002653"
     CDS             complement(4943..6199)
                     /gene="nupG"
                     /locus_tag="ACHKXP_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO7647630.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGRVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6401..7480)
                     /gene="mltC"
                     /locus_tag="ACHKXP_002654"
     CDS             complement(6401..7480)
                     /gene="mltC"
                     /locus_tag="ACHKXP_002654"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO7647631.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7545..7820)
                     /gene="yggX"
                     /locus_tag="ACHKXP_002655"
     CDS             complement(7545..7820)
                     /gene="yggX"
                     /locus_tag="ACHKXP_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO7647632.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7848..8900)
                     /gene="mutY"
                     /locus_tag="ACHKXP_002656"
     CDS             complement(7848..8900)
                     /gene="mutY"
                     /locus_tag="ACHKXP_002656"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO7647633.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9061..9780
                     /gene="trmB"
                     /locus_tag="ACHKXP_002657"
     CDS             9061..9780
                     /gene="trmB"
                     /locus_tag="ACHKXP_002657"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO7647634.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9780..10106
                     /gene="yggL"
                     /locus_tag="ACHKXP_002658"
     CDS             9780..10106
                     /gene="yggL"
                     /locus_tag="ACHKXP_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO7647635.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10290..11009
                     /gene="yggN"
                     /locus_tag="ACHKXP_002659"
     CDS             10290..11009
                     /gene="yggN"
                     /locus_tag="ACHKXP_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO7647636.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11185..12231
                     /gene="ansB"
                     /locus_tag="ACHKXP_002660"
     CDS             11185..12231
                     /gene="ansB"
                     /locus_tag="ACHKXP_002660"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO7647637.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKTGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12348..13355
                     /gene="yggM"
                     /locus_tag="ACHKXP_002661"
     CDS             12348..13355
                     /gene="yggM"
                     /locus_tag="ACHKXP_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO7647638.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13510..14646)
                     /gene="hemW"
                     /locus_tag="ACHKXP_002662"
     CDS             complement(13510..14646)
                     /gene="hemW"
                     /locus_tag="ACHKXP_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO7647639.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14639..15232)
                     /gene="rdgB"
                     /locus_tag="ACHKXP_002663"
     CDS             complement(14639..15232)
                     /gene="rdgB"
                     /locus_tag="ACHKXP_002663"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO7647640.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(15240..15530)
                     /gene="yggU"
                     /locus_tag="ACHKXP_002664"
     CDS             complement(15240..15530)
                     /gene="yggU"
                     /locus_tag="ACHKXP_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO7647641.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(15527..16093)
                     /gene="yggT"
                     /locus_tag="ACHKXP_002665"
     CDS             complement(15527..16093)
                     /gene="yggT"
                     /locus_tag="ACHKXP_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO7647642.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(16111..16815)
                     /gene="yggS"
                     /locus_tag="ACHKXP_002666"
     CDS             complement(16111..16815)
                     /gene="yggS"
                     /locus_tag="ACHKXP_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO7647643.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMQAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16833..17813
                     /gene="yggR"
                     /locus_tag="ACHKXP_002667"
     CDS             16833..17813
                     /gene="yggR"
                     /locus_tag="ACHKXP_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO7647644.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHRVGEGRL"
     gene            17862..17990
                     /locus_tag="ACHKXP_002668"
     CDS             17862..17990
                     /locus_tag="ACHKXP_002668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647645.1"
                     /translation="MSDATLTRLIRPTAAARCVLDKVFTQHPAKWPDDGNDYPPGF"
     gene            complement(17997..18413)
                     /gene="ruvX"
                     /locus_tag="ACHKXP_002669"
     CDS             complement(17997..18413)
                     /gene="ruvX"
                     /locus_tag="ACHKXP_002669"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO7647646.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(18413..18976)
                     /gene="yqgE"
                     /locus_tag="ACHKXP_002670"
     CDS             complement(18413..18976)
                     /gene="yqgE"
                     /locus_tag="ACHKXP_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO7647647.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(19085..20035)
                     /gene="gshB"
                     /locus_tag="ACHKXP_002671"
     CDS             complement(19085..20035)
                     /gene="gshB"
                     /locus_tag="ACHKXP_002671"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO7647648.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(20048..20779)
                     /gene="rsmE"
                     /locus_tag="ACHKXP_002672"
     CDS             complement(20048..20779)
                     /gene="rsmE"
                     /locus_tag="ACHKXP_002672"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO7647649.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQLDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(20859..21566)
                     /gene="endA"
                     /locus_tag="ACHKXP_002673"
     CDS             complement(20859..21566)
                     /gene="endA"
                     /locus_tag="ACHKXP_002673"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO7647650.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(21661..22158)
                     /gene="yggI"
                     /locus_tag="ACHKXP_002674"
     CDS             complement(21661..22158)
                     /gene="yggI"
                     /locus_tag="ACHKXP_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO7647651.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(22235..23629)
                     /gene="galP"
                     /locus_tag="ACHKXP_002675"
     CDS             complement(22235..23629)
                     /gene="galP"
                     /locus_tag="ACHKXP_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO7647652.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(24053..25207)
                     /gene="metK"
                     /locus_tag="ACHKXP_002676"
     CDS             complement(24053..25207)
                     /gene="metK"
                     /locus_tag="ACHKXP_002676"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO7647653.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            25263..25514
                     /gene="yqgD"
                     /locus_tag="ACHKXP_002677"
                     /pseudo
     CDS             25263..25514
                     /gene="yqgD"
                     /locus_tag="ACHKXP_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
     gene            25625..25768
                     /gene="yqgG"
                     /locus_tag="ACHKXP_002678"
     CDS             25625..25768
                     /gene="yqgG"
                     /locus_tag="ACHKXP_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO7647654.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            25862..25993
                     /gene="yqgB"
                     /locus_tag="ACHKXP_002679"
     CDS             25862..25993
                     /gene="yqgB"
                     /locus_tag="ACHKXP_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO7647655.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            26002..27978
                     /gene="speA"
                     /locus_tag="ACHKXP_002680"
     CDS             26002..27978
                     /gene="speA"
                     /locus_tag="ACHKXP_002680"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO7647656.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGIFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            28116..29036
                     /gene="speB"
                     /locus_tag="ACHKXP_002681"
     CDS             28116..29036
                     /gene="speB"
                     /locus_tag="ACHKXP_002681"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO7647657.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(29242..30000)
                     /gene="loiP"
                     /locus_tag="ACHKXP_002682"
                     /gene_synonym="yggG"
     CDS             complement(29242..30000)
                     /gene="loiP"
                     /locus_tag="ACHKXP_002682"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO7647658.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGNLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            30278..32269
                     /gene="tkt"
                     /locus_tag="ACHKXP_002683"
     CDS             30278..32269
                     /gene="tkt"
                     /locus_tag="ACHKXP_002683"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7647659.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT         7893 a         8218 c         8591 g         7857 t
ORIGIN      
        1 aaaacaaaaa atatctaaaa ataaagcatg aaaacaaaat tcctccggac ctggaggccg
       61 gaggagaact tgtggattga gagaaaatat cagaacaagt gttcttaacc ctgactctct
      121 tgcctggaaa gcgaatcaat gtaatctgcg taccattgca tcatttcacg gcgaccatcg
      181 agatactggg catgattata ggtacccctg atggcgtttt tatccacatg agctaactgc
      241 atttcaatcc aggcagaatt aaagccctgt tcgtgcagaa tggtactcat ggtgtgacga
      301 aaaccatgac cggttgccct gccatcgtaa ccgatacgtt tgagcaccat attgatgctg
      361 gcttcgctca ttggcttgtt gatatcacaa cgtcccggga aaaccagccg gtattgtccg
      421 gagaaagttt tgagctgcag cagaatgtca gtggcctgtc gtgacagtgg tacgacatgt
      481 ttacgtcgca ttttcatgcg gctttcaggg attgtccaca gggcattatc aaaatcaatt
      541 tctgaccatt cagcggaccg tagttcaatc gttctgaggc ctgtcagaag cagcagattt
      601 gtcgccatgc gggtaacagg actgccatga tattcagcga gcttccggag aaaaacaggg
      661 agctcatctg ctctcaggtg ggggaaatgt gcagcttttg gcgccgccag cgtactggtc
      721 agttcagatg ccggattgac agtggctcgt ccggtggcga tggcataacg aaaaatctga
      781 ttacagtatt gacgcacctt acgaagtttt tccagcgagc cacgtttttc tatgcggcgc
      841 agaatttcca gcatttccag cgggaggata tcagtaacgc ttcgtttgcc cagatacggg
      901 aaaatatctt tatccagagc ctccagtacg cttctggcgt aactttcaga ccatgacaaa
      961 gatttatgct gatgccattc cgtagcaagg gcatggaacg tattggtggc agagattttc
     1021 tgagtgagtt tttcctttcg cttcttttcg cccgggtcac caccttcgga gatgattttt
     1081 cgggcatcgt cgcgtttgat tctggcctca gcaagggaga cgtcgggata gacgccgagg
     1141 gccagttttt tctcttttcc tgctatccgg tatttaagcc gccagtagcg ggcacccgtg
     1201 gtttttacca gcagatataa accacgttcg tcagagagtt tatagtcttt ttctttaggt
     1261 ctggctgttt cgacctgtct ggctgtaagt gccatatggg ggctcctgaa actgactgaa
     1321 cacaatgagc ccccaccggg gcccccaatg aacgcttgat tgagagagag ttcagtagag
     1381 tctggtggag ttcagaatgt tcagattgct tgtaacgatg tgattatact tgagtttcgt
     1441 ggagttcggg agatgaatct ggaagtgaaa gtggtgcccg gactcggaat cgaaccaagg
     1501 acacggggat tttcaatccc ctgctctacc gactgagcta tccgggcaac ggggcgcatt
     1561 aaacctgatt cgcctcgtct cgtcaatcaa atttcttcaa tttactgcag actgcacaat
     1621 ctatcatcac tttgccgata ttgcacgcat tgtcaggcaa accaggcggt ccaggcggcg
     1681 gaaagcggca ttgccagaag gagtgctgga agcatgttga ccaccgggaa cattttaatg
     1741 ccacagatgc gtaatccggt tgccagcagc aataaaccgc ctacggcgct gaagtctgcc
     1801 atcatcgacg gtgtggtcag cggtaaaatc agcgcggcag cccacgccag cgttaactgg
     1861 atgatcagta atgggatact aatcaccgat accgcaatac ccagcgagca ggcgaagatc
     1921 atcgccgtaa agaaatcaag aaatgacttg gcgattaaaa tactcggatc gccggtcatc
     1981 ccttcgttca tcgccccgaa gatcccggtg ccgctggcgc aaaacaggac aataatcgcg
     2041 acataattct gaataaaaga ttcatgcgct ggcttcttac gtgagtgacg aaacagattt
     2101 tgtgctttgg cgactgctgt attgacacct ttctccagca aacaaatttc gccaattaga
     2161 gcgccaagta gcgtagctaa aaccatcgct ggaaggttgg cacatttcac cactagtaaa
     2221 ataccaatcc ccagcgatgc cagaccaaaa atagatgtca tggagacgcg gatacgttcc
     2281 ggtaagcgtt ggctgagcag tgcgccgaga acgccaccca gtaatactgc actagcgttg
     2341 ataaaagggc cgatgaccac aagagttcct gttaactgta ttgtttgatt ttcttattat
     2401 gaccgtttgg tgccgtcgat ggcttgtttg ttcgcaaagt cctggcttgc acgctttagc
     2461 gaaaggtgcc atgattgcgc gaattttctc ctctctgtac ggagtttgcc cgatgcacgc
     2521 catctcctta cattctctcg cttatcgccg tttcgcgcga aacgtttccc tttctatgtt
     2581 actgctcatg cggtgaagtt aacgcacgct cactgcagga caacagtaaa atcagagcgt
     2641 ttctgctttt actgatgtct ggcggtcgga gctggtgacc agtttgaccc atatctcatg
     2701 gggcagggtt ttccaccttg tccggtattc ttacttcccc gaaacgggtt tgcgcttatg
     2761 aaatcaatga atattgccgc cagtagtgaa ctggtatccc gactttcttc tcatcgtcgc
     2821 gtggtggcgt tgggagatac tgattttacg gacgtcgcgg cagtcgtcat taccgctgcg
     2881 gatagtcgca gtggcattct tgcgttgctt aagcgcaccg gttttcatct accggtgttt
     2941 ttgtattccg aacatgctgt tgaattacct gcgggcgtta cggcggtaat caacggcaac
     3001 gagcagcagt ggctggagct ggaatccgca gcctgtcagt atgaagagaa tttgctgcca
     3061 ccgttttatg acacgctgac gcagtacgtt gagatgggca acagcacctt tgcttgccct
     3121 ggacatcaac atggtgcgtt ttttaaaaag catcctgccg gacgccattt ttacgatttc
     3181 tttggtgaga acgtctttcg cgccgatatg tgtaacgctg acgtaaaatt gggcgatctg
     3241 cttattcatg aaggatcggc gaaagatgcg cagaaattcg cagccaaagt ctttcatgcc
     3301 gataaaacct attttgtgct gaacggcaca tcggcagcga ataaagtggt gacgaatgcg
     3361 ctgttaacgc gtggcgatct ggtgctcttc gaccgtaaca accataagtc gaatcatcac
     3421 ggcgcgctga ttcaggcggg ggcgacgccg gtctatctgg aagcttcacg caacccgttt
     3481 ggtttcattg gcggtattga tgcgcactgt tttaatgaag agtatctgcg ccagcaaatt
     3541 cgcgacgttg cgccagaaaa agccgacctg ccgcgcccgt atcgcctggc gattattcag
     3601 ctgggaacct atgacggcac tgtctataac gcccgtcagg tgatcgatac cgttgggcat
     3661 ctgtgtgatt acattctgtt tgattccgcg tgggtcggtt atgaacaatt tatcccgatg
     3721 atggcggata gctcgccgct gctgttagaa cttaacgaaa acgatccggg gatctttgtg
     3781 actcagtcgg tgcacaaaca gcaggcggga ttctcacaga cgtcgcagat ccataaaaaa
     3841 gataaccata tccgcggaca ggcgcgtttt tgcccgcata agcggttgaa taacgccttt
     3901 atgctccatg cttctaccag ccctttctat ccgctgtttg ctgcactgga tgttaacgcc
     3961 aaaattcatg aaggggagag tgggcgtcgg ctgtgggctg agtgtgttga gatagggatt
     4021 gaagcgcgca aggctattct tgcgcgctgt aagctgttcc gcccgtttat cccgcccgtt
     4081 gttgatggca aattgtggca ggattatccg acatcagtgt tagccagcga ccgccgtttt
     4141 ttcagttttg agccgggggc gaagtggcac ggctttgaag gatatgccgc ggatcagtat
     4201 tttgttgatc cgtgcaagct gttactcact acaccaggta tcgatgccga aaccggcgaa
     4261 tatagcgact ttggcgttcc ggcgacgatt ctggcgcact atctgcgtga gaacggcatt
     4321 gtgccggaga agtgcgatct caactccatt ctgtttttat taactccggc ggaaagccac
     4381 gagaagctgg cacaactggt ggcgatgctg gcgcaatttg aacagcatat tgaggatgac
     4441 tcgccgctgg ttgaggtgtt gccgagcgtt tataacaagt atccggtgcg ctatcgcgac
     4501 tacaccctgc gccagttgtg tcaggagatg cacgatctgt atgtcagttt cgacgtcaaa
     4561 gacctacaaa aagcgatgtt ccgccagcag agtttcccgt cagtggtgat gaacccccag
     4621 gatgcgcata gcgcttatat tcgcggtgac gtggagttgg tgcggattcg tgatgccgaa
     4681 gggcgaattg cggcagaagg ggcgttgcct tatccacctg gcgtgctttg cgtggtaccc
     4741 ggggaagtct ggggtggggc ggttcaacgt tatttccttg cactggaaga aggggtgaat
     4801 ttgttgccgg gattttcgcc ggagctgcaa ggtgtttata gcgaaaccga tgcggatggc
     4861 atgaaacggt tgtacggtta tgtgttgaag taagaataaa aaaaacgggt caccttctgg
     4921 cgacccgttt ttctttgcgt aattagtggc taaccgtctg tgtgcctgtc gggacacgaa
     4981 cgtgtttata tttgaacatc gccatgaacg cgaaggccag aaccacggag taaccagcga
     5041 aaatcaacca tacggtctgc cagtcggtaa tgccgttttg ggtgtacatc tcaacaactc
     5101 taccgctcac gatgccgccg aggatacagc cgaagccgtt agtcatcatc aggaacatcc
     5161 cttgtgcact ggcgcgaatt gccgggctaa cttctttttc gacaaacacc gaaccagaga
     5221 tgttgaagaa gtcgaatgcg caaccgtaaa cgatcatcga cagtaccagc agtacagtac
     5281 cgaacggagt cgggtcgccg taagcaaaca gcgcaaaacg caggatccac gccacaatac
     5341 tgatcatcat tacgttctta ataccgtagc ggcttaagaa gaacgggatg gtcagaatga
     5401 acagggtttc agagatctgc gaaatcgaca tgatgattga cgcatgctgc acaataaagc
     5461 tgctggcaaa catcggatct ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg
     5521 taatctgcag ttccgcgccc agcagcattg agaagataaa gaagattgcc atacgcttgt
     5581 ttttaaacag cgcgaatgca tcgaggccca gcagggttgt ccagctctga ttcgcttgct
     5641 gtttagcaac cggaatatgc ggcagagtca gggtaaacag aaccagaatg gcggaaagtg
     5701 ctgcgccaat atacagctgc atgtggctta attcgaagcc agacaggctc accacccaca
     5761 ttgccatgat aaagccgatg gtgccccaga tacggattgg cgggaagtca gtaacgatat
     5821 ccatcccggc attttgcagg cgatagtaag agatggtgtt gattaaccca agcgttggca
     5881 tataagcaaa cgagttaatc aatatcacaa ggaacatcgc ctccggcgtc gtgacctgtg
     5941 ccgccatgaa cagcgtgata gcgccaatgg tgtggcaaat ggcatatacc catttcgcac
     6001 ttaaccattt gtcggccaca atccccagca gcgcaggcat aaagaccgcg gcgataccca
     6061 gtgagctata aactgcgcca atagaagcac cgtcaaactt cagggtaaca aacatatagg
     6121 aaccgagggt cgtcagccaa cttccccaca gacagaactg cagaaaagag aggattttca
     6181 gctgcagctt aagattcatg ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa
     6241 agcacatttg accgtgcgag atgatgggat tctatcaact tcgcggactg gttttttgtt
     6301 acctgtggca aataattgca aacattccca atttgcaaaa cgaaattgtg atgtggataa
     6361 catttttgcc cctgagcatc gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt
     6421 tttgcgcggt attcacttta taaagataac ggcgagattc cgcagaggga tggcgggtcg
     6481 tcagcgtctg ataaacatcg cccggcgtca tggtgttaat aatattggca gcctgaatct
     6541 tatcattcga aaagactcgc agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg
     6601 cataacgccg cgatgttggg ttatcaattc cgccgagata aacattgttc agcatcgcca
     6661 gatacgcggt gccggtatca atattgctgg caggatcaaa caagaaactg cggctcggcg
     6721 tgccggattt cccctgcgag cggaacacat ctttcccggc agtatgttgt accacctgca
     6781 ttaatcccag cgcatcggaa cggctgaccg catacgggtt aaaggaagat tcggtctgca
     6841 taattgccag aatcagcgac tcatcaacgc catatttccg tgacgcctgg cggaccatgc
     6901 cgagatattt gtgcgcacgt ttatcaaggt ggttcggcac catgttaatg gtgacgctgt
     6961 agatgatacg cagcccgttg ctgcggctct tcagacggtt tttcagcaga taatccgcga
     7021 agttgcttgc gcgaccttcc cagcgaatcg gctgcccggt gttgtccacc acctgaccgt
     7081 aaaggaaagg ttctttcgaa atcgtaatat catcaacgtc ggaatagaga tcgaccgaac
     7141 tcggatcgtc acccatcaat aacgttttga taattgcccg gcgcaaatgc gcggcaggtt
     7201 ctgtcccggc gatggtttcg atagtaatcg taccgtcatc gaagttgatg tggctgcggg
     7261 tctgatattg atcggtgtat ttcacgtagt ccttaggacc agcgatcacc acctctttga
     7321 agccccagat gttctcaata ttgtgggcaa attgccccat cagaatatca aaaccgttgg
     7381 tatctttgac ccaggcttcg ttataggtat cgcctttttt ggtcgtcgaa caggagatga
     7441 gcaacggcgc aatcaaagcc agcgcgagat attttttcat cattccgggt gcgtgttgtg
     7501 tttgttttta tgcaagttgc cggaggcgtg ctccggcact gtttttattt tttatcttcc
     7561 ggcgtatagc cctcgatatg cacctcttta ccctcgaaca ggaagttgac catctcctgc
     7621 tcaagcagct tgcggtgctc ggcattcatc atgttgagtt tcttttcatt aatcagcatg
     7681 gtttgcttgt gctgccactg cgcccaggct tctttggaga tctcgttata gatgcgtttt
     7741 cccagctcgc cggggtacag ctgaaaatcc tgaccttctg cttcacgttg caggaaagta
     7801 caaaaaatcg ttctgctcat aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg
     7861 ccagtgcgta actgctgtaa caaacgctcc acgggagccg ccaggccaac tgacggcggt
     7921 tgcgctaagt tataccagag cgcattgcct tcatccatgc agccggtgaa tgacgacacg
     7981 ggaagccaca taggcacaat atctaagtgg aaatggctga aggtatgccg aaacgcggtc
     8041 agttgcgtca ggttatcggc agcaatctgc cgttgcgcca gccactgccg caaactttct
     8101 tcgtcggcaa actgcgggaa acagtataaa ccgccccaca atccgctcgg cggacgctgc
     8161 gccagcaata cttcatcttc gtgctgtaat agcaaaaagt agccggtgcg ctccggcagc
     8221 gtctgtttcg gttttttgcc cggataaagc gcccagctat tgttggcggc ggcaatacat
     8281 ccgttttgta gcggacagag cgaacatttc ggtttcgagc gcgtacaaat catcgcaccc
     8341 aaatccatca tcgcctgatt aaaccgttcc acgccaaccg cgggcgtcac ctgctcgctc
     8401 aaactccata atttattctc gacctctttt ttccctggcc agccgcttac agcatagcag
     8461 cgcgccagca cccgtttgac gttaccgtcg agaatcggaa agtgcttacc cagagaaagc
     8521 gagagaatcg cgcctgcggt ggaacgcccg acgcccggca gtgctgcaac ttcctcaaag
     8581 gtttccggga atttaccgcc gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg
     8641 cgcgcgcggg cgtaatagcc aagcccggtc cacaagtgga gaacttcgtc gagcggcgca
     8701 ttggcgagat cggtcaccgt cgggaagcgc gccataaagc gttcaaaata ggggataacg
     8761 gtcgcaacct gagtttgttg caacatcact tctgagagcc atactttgta gggcgtcttg
     8821 tcaatttgcc agggcagagt ttttcgcccg tatttatcgt accagtccag aacctgggct
     8881 gaaaattgcg acgcttgcat ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca
     8941 cagcgacggc agggtgtaaa ccggaacttt ccgcagccac ggccttatca tgcttgcatc
     9001 cggcaactaa ctttggataa tgcccgtttt cagaacactt tcacaagcga ctaaaccttt
     9061 atgaaaaacg acgtcatttc accggaattt gatgaaaacg gccgcccact gcgccgtatc
     9121 cgtagttttg tgcgccgcca ggggcgactg accaaaggcc aggaacatgc gctggaaaac
     9181 tactggccgg tgatgggcgt tgagttcagc gaagatatgc tggatttccc cgcgcttttt
     9241 ggccgtgaag cgccggtgac gcttgagatt ggttttggca tgggggcgtc gctggtggca
     9301 atggctaaag atcgccctga gcaggacttc ctcggcattg aagtgcattc accgggcgtt
     9361 ggtgcgtgcc tggcttctgc gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac
     9421 gatgcggttg aagtgctgca taaaatgatt cctgacaatt cattgcgcat ggtgcagctc
     9481 tttttccctg acccgtggca caaagcgcgc cataataaac gccgtatcgt tcaggtgccg
     9541 tttgccgaac tggtaaaaag caaactgcag ctggggggcg tattccatat ggcgaccgac
     9601 tgggaacctt atgcggaaca tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac
     9661 ctgtcagaga gcaatgatta cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa
     9721 caacgtggtc atcgtcttgg tcacggagta tgggacttaa tgttcgagag ggtgaaataa
     9781 tggcaaagaa ccgtagccgt cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat
     9841 taggattttc ggtggcatgg cgattcccgg aaggtacatc ggaagaacag attgataaaa
     9901 ccgttgatga ttttattaac gaggttatcg aaccgaacaa actggccttt gacggcagcg
     9961 gttatctggc ctgggaaggt ctgatctgca tgcaggaaat cggcaaatgc accgaagaac
    10021 atcaggcgat tgtgcgtaag tggctggaag agcgcaaact ggatgaggta cgcaccagcg
    10081 aacttttcga cgtttggtgg gactaagaaa gcatacgggc gatgacaaat gcaaaactgc
    10141 ctgatgcgct acgcttatca ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg
    10201 tttacgccgc atccggcatg gaaaacgcgt actttgttat caatctgggg ccagcaaatg
    10261 ctggcctgat tttttcttga gggaagacta tgatgcgcaa aatgctgctg gcggcagcac
    10321 tttcagtgac ggcaatgacc gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg
    10381 atgtgattgt cagcccgcaa accgtgcagg tgaagggcga aaacggcaat ctggtgatca
    10441 cgccagacgg caacgtgatg tataacggta agcaatattc cctgaatgcc gcccagcgcg
    10501 agcaggcgaa ggattatcag gctgaactac gcagcacgct gccgtggatt gatgaaggcg
    10561 cgaaaagccg cgtcgagaaa gcccgtattg ctctggataa aattatcgtt caggagatgg
    10621 gcgaaagcag caaaatgcgc agccgtctga ccaaacttga tgcgcagctg aaagagcaga
    10681 tgaaccgcat tattgaaacg cgcagcgatg gcctgacgtt tcactataaa gccattgatc
    10741 aggttcgcgc cgaaggccag caattagtga atcaggcaat gggtggaatt ttacaggaca
    10801 gcattaatga aatgggcgcg aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg
    10861 tgctgggaag cctggggggg ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa
    10921 aagatttcca gcagtttggc aaagatgttt gtagccgcgt tgtgactctg gaagatagcc
    10981 gcaaagccct ggtcgggaat ttaaaataat cctctatttt aagacggcat aatacttttt
    11041 tatgccgttt aattcttcgt tttgttacct gcctctaact ttgtagatct ccaaaatata
    11101 ttcacgttgt aaattgttta acgtcaaatt tcccatacag agctaaggga taatgcgtag
    11161 cgttcacgta actggaggaa tgaaatggag tttttcaaaa agacggcact tgccgcactg
    11221 gttatgggtt ttagtggtgc agcattggca ttacccaata tcaccatttt agcaaccggc
    11281 gggaccattg ccggtggtgg tgactccgca accaaatcta actacacagc gggtaaagtt
    11341 ggcgtagaaa atctggttaa tgcggtgccg caactaaaag acattgcgaa cgttaaaggc
    11401 gagcaggtag tgaatatcgg ctcccaggac atgaacgata atgtctggct gacactggcg
    11461 aaaaaaatta acaccgactg cgataagacc gacggcttcg tcattaccca cggtaccgac
    11521 acgatggaag aaactgctta cttcctcgac ctgacggtga aatgcgacaa accggtggtg
    11581 atggtcggcg caatgcgtcc gtccacgtct atgagcgcag acggtccatt caacctgtat
    11641 aacgcggtag tgaccgcagc tgataaagcc tccgccaacc gtggcgtgct ggtagtgatg
    11701 aatgacaccg tgcttgatgg ccgtgacgtc accaaaacca acaccaccga cgtagcgacc
    11761 ttcaagtctg ttaactacgg tcctctgggt tacattcaca acggtaagat tgactaccag
    11821 cgtaccccgg cacgtaagca taccagcgac acgccattcg atgtctctaa gctgaatgaa
    11881 ctgccgaaag tcggcattgt ttataactac gctaacgcat ccgatcttcc ggctaaagca
    11941 ctggtagatg cgggctatga tggcatcgtt agcgctggtg tgggtaacgg caacctgtat
    12001 aaatctgtgt tcgacacgct ggcgaccgcc gcgaaaaccg gtactgcagt cgtgcgttct
    12061 tcccgcgtac cgacgggcgc taccactcag gatgccgaag tggatgatgc gaaatacggc
    12121 ttcgtcgcct ctggcacgct gaacccgcaa aaagcgcgcg ttctgctgca actggctctg
    12181 acgcaaacca aagatccgca gcagatccag cagatcttca atcagtacta atcacctcgc
    12241 cccggtatcg tgccggggct ttttcacttc agacttacgt ccattgccaa ttttcattac
    12301 cctaatgata atcaccggaa taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat
    12361 ggattgttgg cacggcactg cttatgttga tgactggtaa tgcctgggca gatggcgaac
    12421 cgccaactga aaatatctta aaagatcaat tcaaaaagca gtatcacggc attctcaagc
    12481 ttgatgctat caccttaaaa aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg
    12541 aaggcgatgt ctcttccagt gacgatctct atacctgggt cggtcagttg gcagattacg
    12601 aactgctcga acagacctgg acgaaagata aaccggtaaa attctcggcg atgttaacca
    12661 gtaaaggaac gcccgcgtct ggctggtcgg tgaactttta ctcttttcag gcggcagcca
    12721 gcgatcgtgg gcgggtggtc gacgatatca aaacgaataa taaatatctg atcgtgaata
    12781 gcgaagattt caattatcgc tttagtcagc ttgaatctgc gttgaatacc cagaagaact
    12841 cgattcctgc gctggaaaag gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga
    12901 aagcagcgga tgcatattgg ggaaaagatg caaacggtaa gcaaatgacc cgcgaagaag
    12961 cctttaaaaa aattcaccaa cagcgtgatg agtttaataa acagaacgat agcgaggcgt
    13021 tcgcggttaa atatgacaaa gaggtttatc aaccggcgat tgcggcatgt cataaacaga
    13081 gtgaagagtg ttatgaagtg ccgattcagc agaaacgaga tttcgatatc aacgaacaac
    13141 ggcgacagac ttttctgcaa tcacaaaaac tcagccgtaa attgcaggat gactgggtaa
    13201 cgctcgaaaa agggcaatat ccgttaacga tgaaagtttc agaaataaac agtaaaaaag
    13261 tcgcgatttt gatgaaaatt gacgatatca accaagctaa tgaacgctgg aaaaaagata
    13321 ccgaacaact gcggcgcaat ggggtgatta agtaagtttg atatagtgca cgatttgtag
    13381 caggatgcgg cgttgacccc gcatccggca atcaacgtct gatgcgacgc ttgcgcgtct
    13441 tatcagacct acaaccgctg ccacatgtcg gctggataag gcgttcacac cgcatccggc
    13501 aatacaagtt tactcagcca gaaaaagctc cagcagcgaa tttaaaaaca gcttcccatg
    13561 ttccgttatc tgccagtaat ccgcacattc ggtgagataa ccctgggcaa tcgcctcgtc
    13621 taactgtggg cgaatcacat cttcgcaaag cccggtatac gcactaaact ctgcgcgtgg
    13681 cgcggcctcc agcaagcgaa agcggttcat aaagaactca aacggcttat ctgcggcttc
    13741 gacatcacgc tggctttcca gataccttcc ttgcataaaa ccacgcggat gacgcgtttt
    13801 agtggtacgc agaatgcgcc catccgggaa ggtcactttg ccgtgcgcgc cgcagccaat
    13861 accgatgtag tcgccaaagc gccagtagtt gagattgtgc tggcactgat aactgggttt
    13921 ggcgtaagcg gaagtttcat attgctgata acccgctgcg gttaataact gatgcccctg
    13981 ttcgaatata tcccacagcg cgtcgtcgtc cggcagcacc ggtggtcgcg aaccaaacag
    14041 cgtattgggt tcgatggtca gttgatacca ggaaagatgc ggcggattca gttcaatggc
    14101 ctggcgtaga tcgccaagcg cctcttccag tgattgatcc ggtagtccat gcatcaaatc
    14161 aaggttaaag ctacgtaacc ctaaaccgct cgccagcttt gccgctcgtt tcgcttcttg
    14221 cgggccatga atgcgcccaa gtcgtttcag cttttcttcg ctaaaactct gcacaccaat
    14281 agagatgcgg ttcacaccag cacgctgata atcgacaaag cgatcggctt ctaccgtacc
    14341 agggttcgct tccatagtaa tttctgcatc cgctgccagc ggcaaacgcg cacgcacgcc
    14401 gtccagcagc gtttgcatcg ccgggccgga aagcaggctc ggcgtaccac cgccaataaa
    14461 gattgtcttt acttcacggc cctgagcgta agccacatca ttgtccagat cgcaaagcag
    14521 atgctgaacg taatcgtcgt gcggcacttc tcctttcaac gcgtgagagt tgaaatcgca
    14581 gtacgggcat ttctgcacgc accacgggat gtgaatgtag agactcagcg gcggtaattt
    14641 aaccattacg taaagcgtcc aacagcagtt tcaatgcctg accacggtgg gaaatggcgc
    14701 tcttttcttc gcgggtcagt tcggcagcgg ttttcccttc ggaaggtacg aagaagattg
    14761 gatcataacc aaagccacca gtgcccgctg gttcacgagt aatcacgccc ggccagctac
    14821 cgtggcacac cagcggagtc ggatcttccg cgtgacgcag atataccagc acgcagtgga
    14881 aacgcgcctg acgttggtcg tccggtacgt ctttcagtgt ttccaacagt ttttgcagat
    14941 tcttttgatc ggtcgcgtct tcaccggaat aacgcgcgga gtaaataccc ggcgcgccac
    15001 ccagcgcatc taccgccaga ccagagtcgt cggcaattgc cggtaaaccg gtcacttttg
    15061 ccgcatggcg cgctttcaga atcgcgtttt cgataaaggt caggccggtt tcttcagcgg
    15121 aatctacgcc gaggtctgtt tgggccacga tatcaagacc gaagtcgcta agcagcgacg
    15181 ccagctcacg cactttaccg gcattgccgg ttgcgaggac aactttttgc ataggatacc
    15241 taattaatta acgccgcgat ttctggcggg atttgttgcg gattaatgat tttaatttgt
    15301 ttgtggcggc caagttcgcc tttttcaatt accacctggc ttttggctac ccggaattgc
    15361 ttaccgagaa acttcaccag atggctgttg gcctggccgt caaccggcgg cgcggtaatg
    15421 gcgactttaa cttcgtcgcc atgtaaaccg acaatagaat cacggctggc tttcggctga
    15481 atatagagcc gtaaaaccag accgtcatca ttaactgtta cggcactcat aacgccatcc
    15541 acagccccgg cagcagcatg tttccggttg cctgtaatac ttctgcgaca cccatattga
    15601 tgacatacag cagcagaacg aggatcatcg gcgagaaatc aatcccaccc attgccggta
    15661 gcaggcggcg aatcgggcgc agcagcggat cggccagctg aatcagcacg tattcaatcg
    15721 gactacgacc ctggcttacc cagctcataa tcgccatcac cagcaggacc cagaaaatca
    15781 gcaagccgat ggttttcagc agaatcagta aaccggcaat ccagatgatt ggcaggaagg
    15841 tcaccacttt aaacagcaca atggctttga taaaactgag aatataggca accagcagcg
    15901 aggcgctgtc aatgggaccc attgccggaa taacgcggcg cagtggcccg ataattggct
    15961 gcgttacctt cactacaaac tgtgagaagg ggttgtaaaa atcacaatga gcccactgca
    16021 tccagatgcg taataacagc accatggtat acagctcaat gaccgttgaa agcaggaaag
    16081 tcaacgtatt catggcgttc ctttaattcc ttatttttta gagtaatcac gcgcaccaaa
    16141 aattgcagtg ccgatacgaa ccatcgtgct acctgccgca atggcggctt gcatatcgtc
    16201 cgacattccc agagagagcg tgtcgatatg cgggtagcgc gttttcagtc cggcaaatgc
    16261 tacagccatt tggcgtgcaa cttcaaactg ccttacatat tctgactcag gcgcagggat
    16321 tgccatcagc ccgcgcagac gtaaacgcgg tagttcagcg accgccgctg ccagctcgtc
    16381 cagttctgcc agttgaatcc cggacttact gttttcatca ctaatattaa tttgaatcag
    16441 tacgttaaga gggggaagtt ctgccgggcg ctgatcgttg aggcgggtag caatgcgcaa
    16501 acggtcgatg gtatgacacc agtcgaaatg ctctgccacc aggcggcttt tattagactg
    16561 caacgggcca ataaaatgcc attctaatcc tgttacgccc agttcctgaa agtggcgaat
    16621 tttatctacc ccttcctgaa cgtagttttc accaaattga cgctgcccgg catcaatggc
    16681 ttctgcgatg gcgctcgcag gttttgtttt actgactgca agcagcgtaa tttcttctgg
    16741 agaacggccg caacgcgttg cagccgctga gattttgtcc cggacctgtg ccaggttatg
    16801 cgcaatatcg ttcattttcc gaggatctta gtatgaatat ggaagaaatt gtggccctta
    16861 gtgtaaagca taacgtctcg gatctacacc tgtgcagcgc ctggcccgca cgatggcgca
    16921 ttcgcgggct aatggaagct gcgccgtttg atgcgccgga cgtcgaagag ctactgcggg
    16981 agtggctgga tgacgatcag cgggcaatat tgctggaaaa tggccagctg gattttgccg
    17041 tgtcgctggc ggaaaaccag cggttgcgtg gcagtgcgtt cgcgcaacgg caaggtattt
    17101 ctctggcatt acggttgtta ccttcgcact gtccacagct cgaacagctt ggtgcgccac
    17161 cggtattgcc ggaattactc aagagcgaga atggcatgat tctggtgacg ggggcgacgg
    17221 ggagtggcaa atctaccacg ctggcggcga tggttggcta tcttaatcaa catgccgatg
    17281 cgcatattct gacgctggaa gatcctgtgg aatatctcta tgccagccag cgatgtttga
    17341 tccagcagcg ggaaattggt ttgcactgta tgacgttcgc atcggggttg cgggccgcat
    17401 tgcgggaaga tcctgatgtg attttgctcg gagagctacg tgacagtgag acaatccgtc
    17461 tggcgctgac ggcggcagaa accgggcatc tggtgctggc aaccttacat acacgtggtg
    17521 ccgcgcaggc agttgagcga ctggtggatt catttccggc gcaggaaaaa gaccccgtgc
    17581 gtaatcaact ggcaggtagt ttacgggcag tgctgtcaca aaagctggaa gtggataaac
    17641 aggaaggacg cgtggcgcta tttgaattgc tgattaacac acccgcggtg gggaatttga
    17701 ttcgtgaagg gaaaacccac cagttaccgc atgttattca aaccgggcag caggtgggga
    17761 tgataacgtt tcagcagagt tatcagcacc gggtggggga agggcgtttg tgacgttgcc
    17821 atgcccgatg cgcaagctta tcgggcctac aaaggcaata aatgtctgat gcgacgctaa
    17881 cgcgtcttat cagacctaca gccgctgcca gatgtgtgct ggataaggtg ttcacgcagc
    17941 atccggcaaa atggccggat gacggtaacg actatccgcc aggcttttaa atcgccttaa
    18001 tatccctgct cgaaatagct ttcgagaata ataaccgcag aggcagagtc aactttgcct
    18061 ttgttgagcg cccgatagcc gccctgttca aacagaccgg aacgggcttc cacggtgcta
    18121 agacgctcgt catggagctt tacttcaaca ccgaaacgac catgaatacg gttggcgaat
    18181 ttacgcgctc tggcagtcaa tggttgctcg gtgccgtcca tattcagcgg caaaccgacg
    18241 atgatttcgt ccggctgcca ctctttcagt aaacgttcga tgaggttcca gtccggcgta
    18301 ccgtcctgtg ctttaattgc aggcaaaggg cgagcggtgc cggtaatgcg ttggccgacc
    18361 gctacgccaa tgcttttggt accgaagtcg aaggcgagta aggttccact catcaggcgt
    18421 gccctgccac accaggcatg gtgagaatat ccacaccaat cagttttgcc gcctcgcgcc
    18481 agcggtcggc aatcggcgtt ttgaacagaa tattcagatc tgccggggcc gttagccacg
    18541 cgttatcgag aatttcttgt tccagttgac ctttctccca ggaggcataa cccagagcca
    18601 ccaatacgtc agacggttgt ttatcggtgc cgagcgtttc cagcacatcg cgggaagtgg
    18661 tcatcaccgt gttgtctgaa atgcgaatgc tggaagcaaa attggagggc ggagtatgca
    18721 aaataaaccc gcgatcttca gccagtgggc cgccgagcat aaccggttta tccagacgga
    18781 ttgattcatc acgcggctcc ggcgtgatct tcagcttttc cagaatccct tcaattttga
    18841 gattttccag cggcttgttg acgatgatcc ccattgcacc attggtatta tgttcgcaaa
    18901 tgtagaccac ggaacgacgg aaaatcggat cctggagagc aggcatggca ataagaaagt
    18961 gatgctgtaa attcattgtc agaggttctg tttcctggtt caaaaaagca acagcgccca
    19021 gtatgcgtgg aaagaagggc gctgtcactc agagtctcaa cgagatcctt ctcgctaagg
    19081 tgggttactg ctgctgtaaa cgtgcttcga tggcatccat taacattccg gtgatcgaca
    19141 ccggaaactc tgcttcaatc tcacgaatac aggttgggct ggtgacgtta atttcagtca
    19201 gacggtcgcc gatgatatcc agaccaacaa aaatcagccc tttttctttc agcgtcggcc
    19261 cgatctgacg ggcgattttc cagtcacttt ccgtcagcgg acgaggttca ccgcgaccac
    19321 cggcagccag attgccacgg gtttcgcccc cctgcggaat acgcgccagg cagtacggta
    19381 ccggctcgcc atccaccacc agcacgcgtt tgtcgccatc tttaatggct ggcaggtaat
    19441 tttgcgccat gcagtagcga gtgccatgct cagtcagggt ttcggcaatc acgccgaggt
    19501 ttggatcgcc ttctttcacg cggaaaatcg acgcgccgcc cataccgtcc agcggcttaa
    19561 gaatgatgtc gctgtgtttc tcccagaacg cttttagctg cgctttattg cgcgtaacca
    19621 gcgtttctgg cgttaagtca gagaaccagg cggtaaacag tttctcgtta cagtcgcgca
    19681 ggctctgcgg cttgttaacg atcagcgtcc ctttctcttc ggcacgttcc agaatatagg
    19741 tcgcgtagat aaactcggta tcaaacggcg ggtctttacg catcaggatc acatcgagat
    19801 cggccagcgg cagatcctgt tcaccgacga acgaaaacca ctcttcgtag ttctgcttca
    19861 cgttcagcgt gcgggtatgg gcgcgggctt cgccattgat cagatacaga tcgcccatct
    19921 ccatatagtg aagttcgtaa ccacgacgct gtgcttccag caacatagcg aaactggaat
    19981 ctttcttgat gttgatgttt gcgatggggt ccatcacgat gccgagcttg atcattattc
    20041 ttctccgtta gcccaaatcg ccaaatcgta cttgtagcgc ggtaatggcg gtgagcgcag
    20101 ttgtctctgt acgcaaaacg cgaggtccca acaggatatc agtaaattga tagcgggcag
    20161 tcatggcaat ttcatctgcc gataaaccgc cttccgggcc aatcagcagg cggacgcgtt
    20221 caaccggtaa cggcaacgta ttgatgctgt tactggcgcg cgggtgaaga ttcagtttca
    20281 gtccttcatc ctgctctgca caccaggctt ccagatccat cgctggacgg atttccggca
    20341 cccggttacg accacactgc tcacaggcag caattgcaat cttctgccac tgctggagct
    20401 tcttgttcag acgttcacta tccagtttaa cgccgcagcg ctcagaaaaa agtggcgtaa
    20461 tgaggcttac accgagttcg atcgatttct ggatcgtaaa ttccattttt tcaccacgcg
    20521 acatcacctg accgaggtga atatgcagcg gagattcgcg atcgtcgagc tggccttcca
    20581 gcaccttcac ttccacgctt tttttgctgg cgctggtaat ttcggcgtca aagacctggt
    20641 tgctaccgtc aaacaattgc aacgcctgcc ccggccccat gcgcagtacg cgcccgatat
    20701 ggttggcggc atcttcgcaa agcgcgatgt gagaatggct ggtcagtggt tcaggatgat
    20761 aaatgcgggg gatacgcata gttaaaaatc cgcgtcgtct ccccacgcgg ttgtacgcgt
    20821 ggggtagggg ttaacaaaaa gaatcccgct agtgtaggtt agctctttcg cgcctggcaa
    20881 gcgcgttgca catacgggtt atgattgccc tgcaccttcg cgatgcgttc atcgcgctcg
    20941 cactcccagt cggtaaccgg atacatcttg ttccatgcgt tgaacagctg cgtttgctgg
    21001 cgagagagtg tcaggttgta ttggtcgcgc atatagaagt aggtgcgcgc aatggcaccg
    21061 cgtgcacgcg ctggtggttc ggcagctttt tctttgaaat cgaccttcat ggcgcattga
    21121 ccgtactggc cttcgccgcc attccactgg ctgtacataa agttgccgcg atcgccattc
    21181 acctcaccga ctgacggctg caggttatgc atatcgcttt ccatcttgcg atagaccgga
    21241 tctttagcgc agtttttacg tccaccgtcc tgccagcact ggcgctggtg accgaattgc
    21301 caggcgggaa cgacgtgttc ccactctacg cggctggcgc ggttttcatt tttgcgcacc
    21361 tgatagccgc acgattgcag atcaacaacg ccttttttgc cctgccagtt aattttacat
    21421 ccgcaataaa acgtaccggg cgcgtcagcg tggactttta ccgccgcggc tttcgcctga
    21481 gaaaaactat tgataccttc ggccaacgcc gggccggaaa atgctgcgct cagtaccacc
    21541 gcagcaatag acaaataacg gtacatcact tactccgtgt taaaacgagc cagcaacgta
    21601 gcgaaagttg ttttggtatg caatcagcca gatcaggaaa gttcctgata gttcagatgg
    21661 ttattttgcg accagttgtt cgccgcagtg aacacagcga tagacggctt cgccacgaac
    21721 tacgcgatta tggcggcgta cggtaagctg atgctcctgg cacttgcagc ggtaggggaa
    21781 ggtgttgcga cgcacggatt gcagttcgaa ctgatgcgta cgacgggcgg gaacacccag
    21841 cacgttttcc atcatccact tccactcttt gccatgtggt gctacgcgcc cgaaatgttt
    21901 ccataccagc aaatgtgcca gttcgtgcgg caccacttct tcaataaagg cttcactgtt
    21961 ttccaacagc aaaacgggat tgaggcggat ttcatagctt tccagccagg ctgttccggc
    22021 ggaggtgccg cgctgggtgt aagagagttt tggctccggg tagttacgcc ccaatttcag
    22081 gttagcctgg gcgagttttt cccgcaggcg acgcataacg gcctgttgga tggcgatagg
    22141 gagacgggat gttttcatag cggcagagga tagagcgaag aaaagatgac tgcaagaggc
    22201 ggcttcctcc gcgacgggag gaagcctgag gagattaatc gtgagcgccg atttcgcgca
    22261 gtttacgacc tttcatcaga ttacgttcaa tatgttccag cgaaacgtgt ttggtttccg
    22321 gtaccagcca caatgtcagc aggataaaca gtacgttcag agccgcgtac acccagaagg
    22381 tgttggcgtt acccagcgtg ttgagcatgg tcaggaacgt tgcgccaacg atcatgttgg
    22441 caatccagtt ggtggcggtg gagcaggtga tgccaaaatc gcggcctttc agcggctgaa
    22501 tttcggagca cagtacccaa atcagcggac cggcactcat ggcaaaaccg acaataaaca
    22561 tcagcagcat ggcgatggcg aaatactgcg ccgacggaga gtggataccg atatgcatca
    22621 ttgtaccgag tacgcccata ccagcagcca tcaccaggaa gcccagcgtt agcgttggtt
    22681 tacgtcccca gcggtcaaca aggccgattg cgataaaggt ggcaagtacg ttggtcaggc
    22741 cgacaatcac tgtcccccac atttgctcgg tggtgttggt ataacccgcc agttcgaaga
    22801 ttttcggcgc gtaatacatg atgacgttca tcccggtgaa ttgctgcatt acctgtaaca
    22861 gtacgccaag gaacaccgcg cggcggaagt tgctattctc tttaaacagc gcccagccac
    22921 tctgtttaac ctgcaaactt tcacggattt catccagttc gcgtttcgct tccgcgctgg
    22981 tgtcacgcag gcgtagcagc acgcgttcgg catcaacaaa acggcgtttg gcggcaaacc
    23041 aacgtgggct gtctggcagg aagaagacac caatcagcag caaaattgcc gggatgataa
    23101 tcacacccag catccagcgc catgcaccgg tgtagctgaa ggcggtatca gaaagataag
    23161 caccgaggat cccgatagtg atcatcaact gatacataga gatcatactg ccgcgaattt
    23221 tttccggcgc aatttcagag aggtacagtg gtgcggtata agaggccaca cccaccgcca
    23281 gccccagcag aacgcgggaa agaatcagta cttcaacgtt tggcgcagcc gcagagaaca
    23341 gcgaaccggc aacaaacaaa attgcgccga tcatcaggct ctttttgcgc ccgagtttaa
    23401 aggagagcca gccgctgccc accgcaccga ctgccgcacc gaacatcatg gagcttacga
    23461 cccattcttg cgtgtgcgaa gtaatctgga attcatctgc aataaacggc agtgcgccag
    23521 caattacacc gatatccagg ccaaagagta atcccgccag agcggcaagg aagcagacga
    23581 aaaacgtcat tgccttgttt gaccgcccct gttttttagc gtcaggcatg atgccctcca
    23641 atatggttat tttttattgt gaattaagat aggtgagtac gacgtaaaaa gatgtgaagc
    23701 aaatcacacc agtgtaatcg cttacactaa catttatctt agttatgatt taagtgcatg
    23761 aaaataattg agttatatca tcatagatta aacgctgtta tctgcaatta agactttact
    23821 gaaaagaaat gtaacaactg tgaaaaccga ataataatat gaaaatatta caaatctttg
    23881 tctgttgtgg ttggtgtaat cggtttcagt tgtgatgata atctgcggtg aaacgtgatc
    23941 aggaagaatg atgttattgt gcgggcggtt tttttgccgg atgcggcgtg aacgcctatc
    24001 caaaagaaaa aggccagcgc gaggctggcc tttgaacgca ggtgaagaaa gattacttca
    24061 gaccggcagc atcgcgcagc agctgcgctt tgtcggtttt ttcccacggg aaatgttcac
    24121 gaccaaagtg accgtatgct gcggtttctt tgtagatcgg gtgcagcaga tccagcatct
    24181 gaatcagacc gtatgggcgc aggtcgaaga actcacgtac cagcagggtc agttgttcag
    24241 aaggcacttt ctcagtaccg aaagtttcta ccatgatgga ggtcggttca gccacgccga
    24301 ttgcgtagga aacctgaatt tcacaacgat cggccaggcc agcagcaacg atgtttttcg
    24361 cgacataacg tgctgcgtag gctgcggaac ggtccacttt tgatggatct ttaccagaga
    24421 atgcaccgcc accgtgacgc gccatgccgc cgtaggtatc aacgataatt ttacgaccag
    24481 tcagaccgca gtcacccatt gggccaccga taacgaaacg accggtcggg ttgatgaaga
    24541 atttggtggc agaagtcagc cattcagcgg gcagaattgg cttgatgatc tcttccatta
    24601 ccgcttcttg cagcgatttc tggtcgatct cttcagagtg ctgagtggaa agcacgacag
    24661 catcgatacc aacgattttg ccgtcgtcat actgaaaagt cacctggctt ttcgcgtccg
    24721 ggcgcagcca cggcagagtg ccgtttttac gcacttcagc ctgacgctgt accagacggt
    24781 gtgcataggt gataggtgct ggcatcagca cgtcggtttc attagttgcg tagccaaaca
    24841 tcagaccctg gtcacccgcg ccctgttcca gcggatcggc acggtcaacg ccctggttga
    24901 tgtcaggaga ctgtttgccg atagcgctca gaaccgcaca ggagttagcg tcaaagccca
    24961 tgtcggaatg cacatagcca atttcgcgaa cggtgttacg ggtgatctct tcgatgtcta
    25021 cccaggcgct ggtggtgatt tcgccgccaa ctaaaaccat gccggttttt acgtaggttt
    25081 cgcaagcaac gcgtgctttc ggatcctgtt cgaggatcgc gtctaaaacg gcatcagaaa
    25141 tttggtcagc aattttgtca ggatgccctt cagagacgga ctcggacgta aaaaggtgtt
    25201 ttgccatatt taatatcacc taaagagaat ttggttagct caaactgttg tgtggatttt
    25261 ctgtggtagc ggatcctacc acgactctgc aggttaaaaa cactggcagt ctgagtgtta
    25321 atcggtatgg atggattaac atctggatgg ctattttagg tcaattcttc accctatttc
    25381 cacttttttt tgaatcgtgt ctcattctgt taaaaacgta gctggaaatt tttcctgaca
    25441 atgccggcat tctgcgtatt tatcttttgc aattttctgc cattgtgggg tataaaacgc
    25501 ggcgcgcggc ttaaataaaa agcacacgac gtttctttcg tgttgccact tccagccggg
    25561 ttgaaatcag agttttggct tgtgggttcg tcttaacagg cggccgtgga ggtgatacga
    25621 aataatgaac cgttgtctgc tgcttaacct gtctcaccgt tctggtgaag attcgttccc
    25681 cgcactctgc atctctgctt tgcatacctg ccgatgttat acccatctcg gcgcttctca
    25741 ggattcaaga gctggttaca gttactgagg actgaacaag ggcgctcttg taaaaacaag
    25801 agttttctcg tggtttcgcc gaactttcac acttacgttc ggttatgtgc ttaataatgt
    25861 tatgaaaaag aaaccggttg cgcagttgga gcgtcagcat tcactgctgg aaaatccatg
    25921 tgcttatggg ttgttatcgc agttccaggc tgcgatagtc gttaactgtt ttacacttaa
    25981 taaaataatt tgaggttcgc tatgtctgac gacatgtcta tgggtttgcc ttcgtcagcg
    26041 ggcgaacacg gtgtactacg ctccatgcag gaggttgcaa tgagctccca ggaagccagc
    26101 aagatgctgc gtacttacaa tattgcctgg tggggcaata actactatga cgttaacgag
    26161 ctgggccaca ttagcgtgtg cccggacccg gacgtcccgg aagctcgcgt cgatctcgcg
    26221 cagttagtga aaactcgtga agcacagggc cagcgtctgc ctgcactgtt ctgtttccca
    26281 cagatcctgc agcaccgttt gcgttccatt aacgccgcgt tcaaacgtgc gagggaatcc
    26341 tacggctata acggcgatta cttccttgtt tatccgatca aagttaacca gcaccgccgc
    26401 gtgattgagt ccctgattca ttcgggcgaa ccgctgggtc tggaagccgg ttccaaagcc
    26461 gagttgatgg cagtactggc acatgctggc atgacccgta gcgtcatcgt ctgcaacggt
    26521 tataaagacc gcgaatatat ccgcctggca ttaattggcg agaagatggg gcacaaggtc
    26581 tatctggtca ttgagaagat gtcagaaatc gccattgtgc tggatgaagc agaacgtctg
    26641 aatgtcgttc ctcgtctggg cgtgcgtgca cgtctggctt cgcagggttc gggtaaatgg
    26701 cagtcctccg gcggggaaaa atcgaagttc ggcctggctg cgactcaggt actgcaactg
    26761 gttgaaaccc tgcgtgaagc cgggcgtctc gacagcctgc aactactgca cttccacctc
    26821 ggttcgcaga tggcgaatat tcgcgatatc gcgacaggcg ttcgtgaatc cgcgcgtttc
    26881 tatgtggaac tgcacaagct gggcgtcaat attcagtgct tcgacgtcgg cggcggtctg
    26941 ggcgtggatt atgaaggtac tcgttcgcag tccgactgtt cggtgaacta cggcctcaat
    27001 gaatacgcca acaacattat ctgggcgatt ggcgatgcgt gtgaagaaaa cggtctgccg
    27061 catccgacgg taatcaccga atcgggtcgt gcggtgactg cgcatcacac cgtgctggtg
    27121 tctaatatca tcggcgtgga acgtaacgaa tacacggtgc cgaccgcgcc tgcagaagat
    27181 gcgccgcgcg cgctgcaaag catgtgggaa acctggcagg agatgcacga accgggaact
    27241 cgccgttctc tgcgtgaatg gttacacgac agtcagatgg atctgcacga cattcatatc
    27301 ggctactctt ccggcatctt tagcctgcaa gaacgtgcat gggctgagca gctttatttg
    27361 agcatgtgcc atgaagtgca aaagcagctg gatccgcaaa accgtgctca tcgtccgatt
    27421 atcgacgagc tgcaggaacg tatggcggac aaaatgtacg tcaacttctc gctgttccag
    27481 tcgatgccgg acgcatgggg gatcgaccag ttgttcccgg ttctgccgct ggaagggctg
    27541 gatcaagtgc cggaacgtcg cgctgtgctg ctggatatta cctgtgactc tgacggtgct
    27601 atcgaccact atattgatgg tgacggtatt gccacgacaa tgccaatgcc ggagtacgat
    27661 ccagagaatc cgccgatgct cggtttcttt atggtcggcg catatcagga gatcctcggc
    27721 aacatgcaca acctgttcgg tgataccgaa gcggttgacg tgttcgtctt ccctgacggt
    27781 agcgtagaag tagaactgtc tgacgaaggc gataccgtgg cggacatgct gcaatatgta
    27841 cagctcgatc cgaaaacgct gttaacccag ttccgcgatc aagtgaagaa aaccgatctt
    27901 gatgctgaac tgcaacaaca gttccttgaa gagttcgagg caggtttgta cggttatact
    27961 tatcttgaag atgagtaagt cctgtgttac ttgaatccgc ttaatttagc ggtgataatc
    28021 cgccacaatt tattgtgaca aatccaaccc ttcctcgtcg ggcctaacga cgcggaaggg
    28081 tttttttata tcgactttgt aataggagtc catccatgag caccttaggt catcaatacg
    28141 ataactcact ggtttccaat gcctttggtt ttttacgcct gccgatgaac ttccagccgt
    28201 atgacagcga tgcagactgg gtgattactg gcgtgccgtt cgatatggcc acttctggtc
    28261 gtgcgggtgg tcgccacggt ccggcagcga tccgtcaggt ttcgacgaat ctggcctggg
    28321 aacacaaccg cttcccgtgg aatttcgaca tgcgtgagcg tctgaacgtc gtggactgcg
    28381 gcgatctggt atatgccttt ggcgatgccc gtgagatgag cgaaaagctg caggcgcacg
    28441 ccgagaagct gctggctgcc ggtaagcgta tgctctcttt cggtggtgac cactttgtta
    28501 cgctgccgct gctgcgtgct catgcgaagc atttcggcaa aatggcgctg gtacactttg
    28561 acgcccacac cgatacctat gcgaacggtt gtgaatttga ccacggcact atgttctata
    28621 ccgcgccgaa agaaggtctg atcgacccga atcattccgt gcagattggt attcgtaccg
    28681 agtttgataa agacaacggc tttaccgtgc tggacgcctg ccaggtgaac gatcgcagcg
    28741 tggatgacgt tatcgcccaa gtgaaacaga ttgtgggtga tatgccggtt tacctgactt
    28801 ttgatatcga ctgcctggat cctgcttttg caccaggcac cggtacgcca gtgattggcg
    28861 gcctgacctc cgatcgcgct attaaactgg tacgcggcct gaaagatctc aacattgttg
    28921 ggatggacgt agtggaagtg gctccggcat acgatcagtc ggaaatcact gctctggcag
    28981 cggcaacgct ggcgctggaa atgctgtata ttcaggcggc gaaaaagggc gagtaagcac
    29041 cagatgcgat gcgcacgggt aaaacgtgcc attaatgtcg gatgcggcgt gaacgcctta
    29101 tccgacctac gttcggcacc cgtaggccgg ataagatgcg ccagcatcgc atccggcaat
    29161 gcgcacaagg taacaaatgt gccattcatg tcagatgcgg cgtgaacgcc ttatctgacc
    29221 tacgttcgac accaccaggc tttacttaat cccatccgcg ctcatgcgat cgcgaatatg
    29281 ctgggcgcgt tcggcggatg caggatggtc gtcaaacatt gagctttggc gaccttcttc
    29341 cagttttgcc agtttttcaa agctggtggc aagacctgcc gggctgatgc cgcgttggcg
    29401 cagaagatcg taagaataat catcggcttc tgcttcctgg cgctgggaga attgcgaatt
    29461 gactaatttc tcgcccagat taccaagttg tgattgagat aaacttccga caatcccgcc
    29521 cgcagaggcc gcagctactc gcacggcatt tgtaccaagt gccacctgca ttcctttttt
    29581 cacatggcct aacgccacgt gccccatttc gtgaccgatc accgcttcga cttcgttatc
    29641 cgtcatcata tccatcagcc cgctatagac gcggatacag ccgttagcca ttgcaaaggc
    29701 gttcacatcc ttcgccatat acactttgta atttaccggc tgaccgttga tattgttgcc
    29761 tagcgcattg gcaatagttg tcagacgttt agcgtattcg ctattggctg gcgcaatcgt
    29821 cgccttgctg tccatctcct gacatgcctg atcgctcagg gttttcacct gcgcatcact
    29881 caaactgtaa gcctgaaaag cttccgctcc tgatgagagc agtccgttgg agtccatatt
    29941 ctggcaacca gtcagtaccg ttgccacgct cattgctacc aataaggcgc gaattttcat
    30001 gttttctctt ccgcacaagg tcaggattat tctaaaaatg cgccgtttgc aggtgaatcg
    30061 acgctcagtc tcagtataag gaaaagcgca gcggacgggc gagtagattg cgcaacatgc
    30121 gagcatgatc cagagatttc tgaagcggca aaaggatgtt ccatgtacat gacgcgcggc
    30181 ttgcggtaaa ttgttggcaa attttccggc gtagcccaaa acgcgctgtc gtcaagtcgt
    30241 taagggcgtg cccttcatca tccgatctgg agtcaaaatg tcctcacgta aagagcttgc
    30301 caatgctatt cgtgcgctga gcatggacgc agtacagaaa gccaaatccg gtcacccggg
    30361 tgcccctatg ggtatggctg acattgccga agtcctgtgg cgtgatttcc tgaaacacaa
    30421 cccgcagaat ccgtcctggg ctgaccgtga ccgcttcgtg ctgtccaacg gccacggctc
    30481 catgctgatc tacagcctgc tgcacctcac cggttacgat ctgccgatgg aagaactgaa
    30541 aaacttccgt cagctgcact ctaaaactcc gggtcacccg gaagtgggtt acaccgctgg
    30601 tgtggaaacc accaccggtc cgctgggtca gggtattgcc aacgcagtcg gtatggcgat
    30661 tgcagaaaaa acgctggcgg cgcagtttaa ccgtccgggc cacgacattg tcgaccacta
    30721 cacctacgcc ttcatgggcg acggctgcat gatggaaggc atctcccacg aagtttgctc
    30781 tctggcgggt acgctgaagc tgggtaaact gattgcattc tacgatgaca acggtatttc
    30841 tatcgatggt cacgttgaag gctggttcac cgacgacacc gcaatgcgtt tcgaagctta
    30901 cggctggcac gttattcgcg acatcgacgg tcatgacgcg gcatctatca aacgcgcagt
    30961 agaagaagcg cgcgcagtga ctgacaaacc ttccctgctg atgtgcaaaa ccatcatcgg
    31021 tttcggttcc ccgaacaaag ccggtaccca cgactcccac ggtgcgccgc tgggcgacgc
    31081 tgaaattgcc ctgacccgcg aacaactggg ctggaaatat gcgccgttcg aaatcccgtc
    31141 tgaaatctat gctcagtggg atgcgaaaga agcaggccag gcgaaagaat ccgcatggaa
    31201 cgagaaattc gctgcttacg cgaaagctta tccgcaggaa gccgctgaat ttacccgccg
    31261 tatgaaaggc gaaatgccgt ctgacttcga cgctaaagcg aaagagttca tcgctaaact
    31321 gcaggctaat ccggcgaaaa tcgccagccg taaagcgtct cagaatgcta tcgaagcgtt
    31381 cggtccgctg ttgccggaat tcctcggcgg ttctgctgac ctggcgccgt ctaacctgac
    31441 cctgtggtct ggttctaaag caatcaacga agatgctgcg ggtaactaca tccactacgg
    31501 tgttcgcgag ttcggtatga ccgcgattgc taacggtatc tccctgcacg gtggcttcct
    31561 gccgtacacc tccaccttcc tgatgttcgt ggaatacgca cgtaacgccg tacgtatggc
    31621 tgcgctgatg aaacagcgtc aggtgatggt ttacacccac gactccatcg gtctgggcga
    31681 agatggcccg actcaccagc cggttgagca ggtcgcttct ctgcgcgtga ccccgaacat
    31741 gtctacatgg cgtccgtgtg accaggttga atccgcggtc gcgtggaaat acggcgttga
    31801 gcgtcaggac ggcccgactg cgcttatcct ctcccgtcag aacctggcgc agcaggaacg
    31861 aactgaagag caactggcaa acatcgcgcg cggtggttat gtgctgaaag actgcgccgg
    31921 tcagccggaa ctgattttca tcgctaccgg ttcagaagtt gaactggctg ttgctgccta
    31981 cgaaaaactg actgccgaag gcgtgaaagc gcgcgtggtg tccatgccgt ctaccgacgc
    32041 atttgacaag caggatgctg cttaccgtga atccgtactg ccgaaagcgg ttactgcacg
    32101 cgttgctgta gaagcgggta ttgctgacta ctggtacaag tatgttggcc tgaacggtgc
    32161 tatcgtcggt atgaccacct tcggtgaatc tgctccggca gagctgctgt ttgaagagtt
    32221 cggcttcact gttgataacg ttgttgcgaa agcaaaagaa ctgctgtaat tagcatttcg
    32281 ggtaaaaagg tcgcttcggc gacctttttt attaccttga tatgtccgtt tgcggacaag
    32341 caatagataa agcgtgttgt agatcacaaa tatttatatg caataaatat caattatgta
    32401 atatgcatca cgatatgcgt attgacattt gttgttataa ctataactca atgttatata
    32461 agaaattaat aattaactgt tttcaaaaca ccggtttccc tgctcaattg ctttcattaa
    32521 accgcacgag tattttattt ttaccgccgg acggtaccc
//
LOCUS       DAZFZC010000049        32553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFZC010000049 DAZFZC010000000
VERSION     DAZFZC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.049"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..513
                     /locus_tag="ACHKXP_002684"
     CDS             <1..513
                     /locus_tag="ACHKXP_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7647660.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            529..1068
                     /locus_tag="ACHKXP_002685"
     CDS             529..1068
                     /locus_tag="ACHKXP_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO7647661.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ACHKXP_002686"
     CDS             complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ACHKXP_002686"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO7647662.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFITVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ACHKXP_002687"
     CDS             complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ACHKXP_002687"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO7647663.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4435..5805
                     /gene="xseA"
                     /locus_tag="ACHKXP_002688"
     CDS             4435..5805
                     /gene="xseA"
                     /locus_tag="ACHKXP_002688"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO7647664.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPAAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFTSRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5802..6017)
                     /locus_tag="ACHKXP_002689"
     CDS             complement(5802..6017)
                     /locus_tag="ACHKXP_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO7647665.1"
                     /translation="MELHCPQCQHVLDQDNGHARCRSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6087..7559)
                     /gene="der"
                     /locus_tag="ACHKXP_002690"
     CDS             complement(6087..7559)
                     /gene="der"
                     /locus_tag="ACHKXP_002690"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO7647666.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7677..8855)
                     /gene="bamB"
                     /locus_tag="ACHKXP_002691"
     CDS             complement(7677..8855)
                     /gene="bamB"
                     /locus_tag="ACHKXP_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO7647667.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8866..9486)
                     /gene="yfgM"
                     /locus_tag="ACHKXP_002692"
     CDS             complement(8866..9486)
                     /gene="yfgM"
                     /locus_tag="ACHKXP_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO7647668.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9504..10778)
                     /gene="hisS"
                     /locus_tag="ACHKXP_002693"
     CDS             complement(9504..10778)
                     /gene="hisS"
                     /locus_tag="ACHKXP_002693"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO7647669.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10889..12007)
                     /gene="ispG"
                     /locus_tag="ACHKXP_002694"
                     /gene_synonym="gcpE"
     CDS             complement(10889..12007)
                     /gene="ispG"
                     /locus_tag="ACHKXP_002694"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO7647670.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12034..13047)
                     /gene="rodZ"
                     /locus_tag="ACHKXP_002695"
     CDS             complement(12034..13047)
                     /gene="rodZ"
                     /locus_tag="ACHKXP_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO7647671.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13332..14486)
                     /locus_tag="ACHKXP_002696"
     CDS             complement(13332..14486)
                     /locus_tag="ACHKXP_002696"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO7647672.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14636..15067)
                     /gene="ndk"
                     /locus_tag="ACHKXP_002697"
     CDS             complement(14636..15067)
                     /gene="ndk"
                     /locus_tag="ACHKXP_002697"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO7647673.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15216..17528)
                     /gene="pbpC"
                     /locus_tag="ACHKXP_002698"
     CDS             complement(15216..17528)
                     /gene="pbpC"
                     /locus_tag="ACHKXP_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO7647674.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAEQGVWSRKQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17529..22490)
                     /gene="yfhM"
                     /locus_tag="ACHKXP_002699"
     CDS             complement(17529..22490)
                     /gene="yfhM"
                     /locus_tag="ACHKXP_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO7647675.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPLFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAVPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGGTWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNALSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVSNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22697..23542
                     /gene="sseA"
                     /locus_tag="ACHKXP_002700"
     CDS             22697..23542
                     /gene="sseA"
                     /locus_tag="ACHKXP_002700"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO7647676.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLVGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24204..24329
                     /gene="timP"
                     /locus_tag="ACHKXP_002701"
     CDS             24204..24329
                     /gene="timP"
                     /locus_tag="ACHKXP_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO7647677.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24360..25136)
                     /gene="sseB"
                     /locus_tag="ACHKXP_002702"
     CDS             complement(24360..25136)
                     /gene="sseB"
                     /locus_tag="ACHKXP_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO7647678.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25278..26561)
                     /gene="pepB"
                     /locus_tag="ACHKXP_002703"
     CDS             complement(25278..26561)
                     /gene="pepB"
                     /locus_tag="ACHKXP_002703"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO7647679.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26739..26939)
                     /gene="iscX"
                     /locus_tag="ACHKXP_002704"
     CDS             complement(26739..26939)
                     /gene="iscX"
                     /locus_tag="ACHKXP_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO7647680.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26951..27286)
                     /gene="fdx"
                     /locus_tag="ACHKXP_002705"
     CDS             complement(26951..27286)
                     /gene="fdx"
                     /locus_tag="ACHKXP_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO7647681.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27288..29138)
                     /gene="hscA"
                     /locus_tag="ACHKXP_002706"
     CDS             complement(27288..29138)
                     /gene="hscA"
                     /locus_tag="ACHKXP_002706"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO7647682.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29155..29670)
                     /gene="hscB"
                     /locus_tag="ACHKXP_002707"
     CDS             complement(29155..29670)
                     /gene="hscB"
                     /locus_tag="ACHKXP_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO7647683.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29766..30089)
                     /gene="iscA"
                     /locus_tag="ACHKXP_002708"
     CDS             complement(29766..30089)
                     /gene="iscA"
                     /locus_tag="ACHKXP_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO7647684.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30106..30492)
                     /gene="iscU"
                     /locus_tag="ACHKXP_002709"
     CDS             complement(30106..30492)
                     /gene="iscU"
                     /locus_tag="ACHKXP_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO7647685.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30520..31734)
                     /gene="iscS"
                     /locus_tag="ACHKXP_002710"
     CDS             complement(30520..31734)
                     /gene="iscS"
                     /locus_tag="ACHKXP_002710"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO7647686.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFITMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31846..32334)
                     /gene="iscR"
                     /locus_tag="ACHKXP_002711"
     CDS             complement(31846..32334)
                     /gene="iscR"
                     /locus_tag="ACHKXP_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO7647687.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
BASE COUNT         7643 a         9058 c         8116 g         7736 t
ORIGIN      
        1 aaatttaaaa aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc
       61 cagtcaaatg ctgacgatca tgctgccgat gtttatcaaa ccgatcaact gaataccaaa
      121 caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac
      181 tcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt
      241 gttatcggcc acaacgtcgg gtctggcagc aattccggaa cgacggcagg tgcagttggc
      301 ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt agtggaaggt
      361 gtttctttaa cctataagga aggcaccaaa gtgtatacct ctacccaggt gggtaaagag
      421 tgccagttta cgacaggttt agccgttgtt attaccacga cgtataacga aacgcgtatt
      481 cagccaaata ccaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt
      541 tttctttgcg ccatcttagc ctccttaagc tatccggcta tcgcctcatc attgcaggat
      601 caactctctg ctgtcgcaga agcggaacag caaggtaaaa atgaagagca aaggcagcat
      661 gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat
      721 gcccaggccg ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag
      781 gataaactgg acgccgaagc ctctgcggac aaaaaacgcg atcaaagtta tgaagatgag
      841 ctacgcagct tagagattca gaaacaaaaa ctggcgctgg caaaagaaga agcccgcgtt
      901 aagcgagaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg
      961 gtgcaatctg aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa
     1021 agcgtgggca aagcagaaga gaacaaatcg gacagctggt ttaattaatc gatgttagta
     1081 acttcaatcc tataattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc
     1141 attcatagtg cagggtcaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1201 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1261 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1321 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtacc
     1381 ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1441 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1501 agaacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
     1561 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1621 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1681 tgtgctttac cggttgcaga cgccgcagat tcgataacgt cagggtagat ggtgccctgc
     1741 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1801 cgaccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1861 cgatcttctg ccggaacgtg aacaatgttc agaccaaagt gatcgccaaa catatccaga
     1921 acctgctctg cttcgttgag gcgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     1981 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2041 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
     2101 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2161 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2221 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgatgaag
     2281 tcggacggaa tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2341 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2401 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2461 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2521 tgcggcgcac gcggactgtt ttcttcagta gtactttccg ggccgccgga aagaataatg
     2581 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2641 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2701 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2761 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2821 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2881 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     2941 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3001 ctttataggc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3061 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3121 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3181 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     3241 cggagaagcg aataccgcca tcagcgataa ccggaatacc ggtgccttcc agggcttcta
     3301 ctgcgtcagc aacagcggta atctgcggaa caccgacgcc agtcacgata cgagttgtac
     3361 agatagagcc agggccaatg ccgactttaa ccgcactgca accagcttct gccagagcgc
     3421 gtgcacctgc agctgttgcc acgttgccgc cgataatttg cagatccgga tatttagcac
     3481 gggtttcacg gatacgttgc agtacacctt ctgagtgacc gtgggaggag tcgatcagca
     3541 gaacgtcaac gcctgcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3601 caaccgctgc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3661 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3721 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3781 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3841 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3901 taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
     3961 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4021 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4081 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4141 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4201 gcagaacggt agagtgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4261 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4321 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4381 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4441 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4501 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tttctaattt cacgcaacca
     4561 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4621 ttccgcaaca gcaaccgccg ggtgaccttc cgcccacagc atgggcaaca agttttagtt
     4681 cgcgccaata ttacgctcta cgagccgcgc ggcgactacc agataatcgt tgagagtatg
     4741 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4801 gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4861 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4921 cgcgatcctt ctctgccggt gatcatctac cctgccgccg ttcagggcga tgacgcgccg
     4981 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtattgatc
     5041 gtcgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5101 cgggcgattt ttaccagccg cattccggtt gtcagcgccg tcgggcatga gacggatgtg
     5161 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     5221 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5281 atggcgatgg attattatct cgccaaccgc acacgtcgct ttacgcaaat tcatcaccga
     5341 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     5401 caaaagcgaa tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     5461 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5521 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgcacagct tagcgccacg
     5581 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5641 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5701 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagta
     5761 aaaaacatcc agccagtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5821 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccacag
     5881 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataaat
     5941 tccccgcagg atcggcaacg cgcatggccg ttatcctgat caagcacatg ctgacattgt
     6001 ggacaatgca gttccatatg aattcctccg gtagccattc cctctacatt catagaggga
     6061 atggcagata aaatacttac ggataattat ttatttttct tgatgtgctt catcagacgc
     6121 ttacgtttac gcatctgggt tggcgtcagg gtgttacgct tattcgcata cgggttttcc
     6181 ccttctttga actgaatacg aatcggcgat cccattacgt ccagcgattt gcggaagtag
     6241 ttcatcaagt agcgcttgta ggaatcaggc aggtctttca cctgattacc gtgaatcacc
     6301 acaatcggcg ggttataacc accggcgtgg gcatatttca gcttcacacg acgaccgcgt
     6361 accagcggcg gttggtgatc ttcaacagcc atcgtcatga tgcgcgtcag catagaggtc
     6421 cccacacgac gggtggagct gtcatacgct tcacgtactg attcaaacaa gttaccaaca
     6481 ccactgccgt gcaaggcaga gataaagtgc acacgagcaa aatcgataaa gcccagacgg
     6541 aagtccagcg tttctttcac ctgctctttc acttcctgac tcaggccatc ccacttattc
     6601 accacaatga caagtgagcg cccactattg agaataaagc ccagcagcga gagatcctga
     6661 tcggaaatac cttcgcgcgc atcaatcact aacatcacca cgttggcgtc ttcaatggcc
     6721 tgcaacgttt tgattacgga gaatttctct acagcatcgg tgattttgcc gcgtttacgt
     6781 acgccagcgg tgtcaatgag cacatactca cgtccatcgc gttccattgg gatgtagatg
     6841 ctgtcacgcg tcgtgccagg catgtcgtaa acaacaacgc gctcttcacc aagaatacgg
     6901 ttagtgagtg tagacttacc tacgttcgga cgacccacaa tcgccagttt gatcggcaga
     6961 ctttgcgggt cgaagtcgtc ttcctcttct tcttcgccgt tctcttccgc ttcaaattgc
     7021 gcccagtatt cagcgtcttc gtcgacttcc tcttgcggtg cgagatcttc catccacggc
     7081 agcagcacat gctccagcag acttaatacg ccacgaccgt gagacgcggc gatcgggtag
     7141 atttcaccta aaccaagcga gtagaaatca accactgcct gatcgggatc cagaccgtca
     7201 gttttgtttg ccaccaggaa ggtcggtttt tcacgggagc gcagatgttt ggcaatcgct
     7261 tcatctgccg gcatcaggcc cgcgcgcgca tccaccataa acagtacgac gtccgcttct
     7321 tcaatcgcca gcagcgactg ttccgccatg cgggtttcta caccgtcttc tgtgccatca
     7381 atcccgccgg tatcaataca gataaactca cggccttcaa tttccgcacg accgtactta
     7441 cggtcacgag tcagacccgg gaaatccgca accagcgcat ctcgggtgcg agttagacgg
     7501 ttaaataacg tggattttcc tacgttaggg cgcccgacaa gcgcgaccac aggtaccatg
     7561 tttaaagcct catttttata aatcatcaga caacgcacgc tatattcgcg tcgttgttaa
     7621 aaacaggaaa acggcccctg tccaggagcc gttttcaaag tgaacgacag agacgattaa
     7681 cgtgtaatag agtacacggt tccgtctttt gcctggatca gcagtttgcc gtcagcggca
     7741 accggttcag tctggaaacc ggaactatca actttttgct gggcaacgaa acgaccatct
     7801 tcgacgttaa tccagtgcag ataaccttca ctgtcaccga ccaccaggtt gccattatac
     7861 agcaccggag aagtcagcag gcgatgcagc agatcgcttt gtgtccacag cgtaacgccg
     7921 ccatcaatgg tcaacgccat cacccggtca ttttgatcga ccagatagat gcgattgccg
     7981 tcgacgatga aatcattcac cgaacccagt tcgcgtttcc acataatctg accactgcgc
     8041 agatcaagcg ccgtcaggtt accattatag gccagcgcga aaacaacgcc gttaacaacg
     8101 acgggagtcg tgtcaacatc gctcagacgg tcaatttcgg tagaaccggt cgcctgggaa
     8161 atacgctgct gccaaatcat ctggccctgt tccatcagca ctgcgctgac gcgaccatta
     8221 tcgcccccca cgacggccgc accaaaagcc gttgccggcg cagactcgcc acgcaaagag
     8281 agcgaaggca tatcgaggtt aactgtccat ttgacagcgc cgtcagcttc gttcagcgct
     8341 tgtaactgac cgttactggt gtggattaac accagaccgt cgctgaccac cgggcgcgaa
     8401 agtgcttcac ccgcgacttt agtttgccat gccacagtac catcgctggt attcagcgcg
     8461 taaacctgcg ccttttcgct gccaatgtag acatgcccac cagacacggt cacaccgcca
     8521 gaaagtaatg caggctcttt agagaaccag ccatctttct cggccaggct gacagaccag
     8581 atttctttgc catcatccgc attcagcgct tttactaaac cagcgcggtc cgctgcatag
     8641 acaacgttgt ccgccagtgc cggatgaaga ttggaataga agttgccaat gccgctacca
     8701 acggaagtgc tccacgccgt ggtcggcgta aactggtttt caacggttgg caatggggac
     8761 atctttacca catcttcttc gctgttaaac agcgaacagc cgcttaaaag ggtaacggaa
     8821 agcagtcctg gcagcagtaa tttacgcaat tgcatcgggt ccctctcaga tggacaaatt
     8881 attaattttc atctgcatca tttcgctcag tgccggagta acatcgcttt tcacgcctgc
     8941 ttcccatgca ctacgcgcac cttgcttatc acctttgctc agcaatgctt caccacgcag
     9001 gtcggcaaca atggcagccc acccttcacc tttgatggta tcaagggttt tcagcgcggc
     9061 atcagcctgc ttgagctgta cctgaacgcg agcaagacgc agatttatca cggctttgag
     9121 attttcatcg ctcgtgtctg ccagcccctg ttgtaactgg gcggcagctt tctctagttc
     9181 atttttgtca acaaattgct gcgcaagttc caaagaagcc agcgcaccat aagtattttt
     9241 attttcagca gcaaattttt ccgccgccgg gatgctatcc ggtttgcctt cgctcactgc
     9301 ggtaaccgca ttttgatagg caagagaagc ggagcgtgca gaatcaacct gatggctgtt
     9361 ccagtagcgc cagccaatca gtgcgccaac gcccaaaatc accccaacag ccagtgcttt
     9421 gccattttca gcaaaaaagc gtttaaccgc ttctacctgg tcgttttcgt tctcgtaaat
     9481 ttccacgctg tccttctcct tccttaaccc agtaacgtgc gcaaatgcgc ggctacgcta
     9541 tcctgcgcaa ctgccgtttg ctcaccagag cgcaaatcct tcactactgc tgtgccgtta
     9601 gccacttcag actcacccag caccacagca acgcgggcac cccatttatc agcacgggca
     9661 aactgtttct taaagttgcc gccgccgtgg ttggtcatca atttcacgcc cggtaattca
     9721 tcacgcagac gctcagctaa tgccatagcc gcagattgtg tatcagcacc tgaagccacc
     9781 aggtatatat cgacaacagg atcggcttta aattccggat taacggcctg tactaacaat
     9841 acaagacgtt cgaggcccat cgcaaaaccg acagccggtg ttgcacgacc gcccagttgt
     9901 tccacaagac cgtcataacg accgcctgca cacacggtgc cctgggagcc gagactgtta
     9961 gtcacccact cgaaaacggt acggttgtag taatccagac cacgcaccag acgctggttt
    10021 acggtgtaag cgatccccgc gctctccagc agtttgcaca gaccggcaaa atgctcacga
    10081 gattcctcgt ccagatagtc acctaatgcc ggagcgtcgt tgagaagcgc ctgcacttcc
    10141 ggattttttg aatccagcac gcgcagcggg ttagtgtaca tgcggcgttt gcagtcttcg
    10201 tccagctttt ctttatgctg ctcaaggaat gccaccagcg catcgcggta attggcgcgt
    10261 gcttccagcg aaccgataga gttcagctca agagttacgt gctcggaaat acccagcgcg
    10321 cgccaccagc gggcagtgag cataatcagt tcagcgtcga tatccggacc ttgcagaccg
    10381 aaaacttcgc agcccaactg atggaactga cgataacgcc ctttctgcgg acgctcgtga
    10441 cggaacatcg gcccgatata ccacagacgc tgttcctgat tgtacagaag accatgctcg
    10501 atgccggcgc gtacacagcc cgccgtccct tcagggcgca gagtcaggct gtcgccattg
    10561 cgatcctcaa aggtgtacat ctctttttca accacgtcgg tgacttcacc aatcgcacgt
    10621 ttgaatagcg gggtctgctc tacaatcggc aagcggattt cactgtaacc gtagctgccg
    10681 agcacgtttt tcagtgtgcc ttcaatgcgc tgccagatgg ccgtttcgcc aggcaggtaa
    10741 tcgttcatgc cgcgaatggc ttgaatgttt tttgccacgt ttattctctt tctgaatata
    10801 aaaatgaacc ctcaacgctt ccctcaatgt ttcgggagcc atgcgggttc aatcatacac
    10861 gggaagcgag gcgcttccca tcacgttatt atttttcaac ctgctgaacg tcaattcgac
    10921 gcgcttcgtc cagctgactg gctttcgcac gaatgcgtgc ttccagctgg tcgatcatat
    10981 cgttgttgtc cagacggtct ttgcgcacgc catcttcata gaggccgctt ttcttgttgc
    11041 cgccggtgac gccgagtgta gaaaccagcg cctcacctgg gccattcacc acgcagccga
    11101 taatcgaaac gtccatcgga gtgatgatat cttccaggcg ttgctccagc gcgttaaccg
    11161 taccgataac atcaaattcc tgacgcgaac aggtcgggca ggcgatgaag ttgatccctc
    11221 gcgaacggat acgcagcgat ttcaaaatat cgaaaccgac tttgatctct tcgaccggat
    11281 cggccgccag cgatacgcgc agcgtgtcgc cgatgccttc agacagcagc agacctaaac
    11341 caatggcgga ttttactgcc ccgctgcgcg caccaccggc ttcggtgatc cccagatgca
    11401 acggctgatc gatctgtttt gccagcaaac gataagactc aacagcgagg aagacgtcag
    11461 acgctttcac gctgactttg aactgatcga agttcaggcg atcgagatga tcaacatgac
    11521 gcatggcaga ttccagcaac gcctgcggcg tcggttcgcc atacttttct tgcagatctt
    11581 tttccagcga tccggcgtta acgccaatac ggatcggaat gtttttatcg cgcgcacagt
    11641 caaccaccat gcgaatacgc tcttcattac cgatattgcc agggttaata cgcagacaat
    11701 cgacgccgta ttccgctact ttcagcgcaa tgcgatagtc gaagtggatg tcagccacca
    11761 gcggcacgtt aacctgctgt ttgatgagtt tgaacgcttc tgccgcgtcc atcgtcggta
    11821 cggatacacg gacgatatca gcgccaacgc gttccagcgc cttgatttga ttgaccgttg
    11881 cttcgacgtc tgtcgtacgc gtattggtca tggactgtac ggcgatggga gcaccatcgc
    11941 caatcggcac attcccaacg taaatacgtg ttgattttct acgttgaatt ggagcctggt
    12001 tatgcatgaa aaatctcccg cgttacccgt ctgttactgc gccggtgatt gttcggcatt
    12061 gagggtcaga cgcgcaacct ggttagttct gataaaacga ctcagatcga caggtttccc
    12121 ttgatactgg atctgtactg cggctggcgc accaattttc agtttgtacg gtgcctggcc
    12181 ggttaagttc aaattaccgt ctttacgctg cataccgcta aacaattttt taccggtagc
    12241 atcagtgacc tccagccagc aatcggcagt aaagttcatc accagcgcat tcggatcagc
    12301 caccggcgtg gtcacgccag cctgatcggt tggcaacggc gcagcaccat ctggcgttgt
    12361 tgctgccgtt ggtgccgggg tcgcggcggt atcaacattt gcctgcgaag gcgaaacaac
    12421 cgcattctgt tgcggatcaa cagctggtgc tggcgcagtt acggcaggtg tttgcgtgtt
    12481 ggttgcggta gtatccacag acgctggcgg cgtggacgtt gtagccgggt ctgtagttgt
    12541 cgacgtattt aatggaacac tctgcccctg ctcgctatta ctgctcagtt ccgccgaaga
    12601 ttgatcggcc atagtggtga tctcttcctg ctgagctttg tggtcttgcc accaccaggc
    12661 accgctcagg ccgataacca caaacaacac cagccaagtg aaggtcatca gccagccgtc
    12721 gcgttttttg cggcgtttac cgagggaaaa actctgcatc ggcgcaactt ttgcagcccg
    12781 aagtggagcc tgcttttcca gccctggcag cagttcttct tctggaatat gtaccagacg
    12841 cgcataagag cggatatatc cgcgcaggaa tgttgaagca agatcggcgg gtgccttatc
    12901 ttcttcaatg tcgcgtaccg tggaaacctt caggcaaagt cgctcggcaa cggcctgctg
    12961 actaagtcct agttgttcgc gagcattacg caggcgagcg ccggtagtaa gtgcttcatt
    13021 ttggtcgtgc gtggcttcag tattcattcg ctgcaggtac gtttaaatga gaattgagat
    13081 gccggtgaac catcatgctc acccacaccg cgaaacatcc gttaagttaa ccgttatcat
    13141 acagtataag accgtctgtg tgctcgtgac aaagcccaca ttcactaagg ctaacttact
    13201 gttgcatcgt tacatactgc cttaaagtca gcaaaaacgc accgttaata ttgaccagac
    13261 aattgcaact taatacttca ttcataaaac attacgccac ggtacataaa gtaaccgtgg
    13321 cgtaatggct atcagaccgc tttaatgtcg atggcttcac cctgcatccg tttacgcagg
    13381 gtacgtttcg tacggtcgat aacatcgccc gccaactgac cacaggcagc atcgatatca
    13441 tcaccacgag ttttacgcac aatagtggtg aaaccgtagc tcatcagcac ttttgagaaa
    13501 cggtcgatac ggctgttcga gctgcgtcca tacggcgcac ccgggaacgg gttccacggg
    13561 atcaggttga tcttacacgg cgtatctttc agcagttccg ccagttggtg cgcgtgttca
    13621 gtgccgtcgt taacgtggtc aagcatcacg tattcaatag tgactcggcc ctgattggcg
    13681 ttggatttct ccagataacg gcgcaccgca gcaaggaacg tttcgatatt gtactttttg
    13741 ttgatcggca caatttcgtc acgaatttcg tcgttcggcg cgtgcaggga aattgccagt
    13801 gcaacgtcga tcatatcgcc cagtttatcc agcgccggaa ctacaccgga agtggaaagc
    13861 gtgacgcgac gtttagacag gccaaaaccg aaatcatcaa gcatgatttc catcgccgga
    13921 acgacgttgt tcaggttgag cagcggctcg cccatgccca tcatcacgac gttagtgatc
    13981 ggacgctgac cggtgacttt tgctgcgccg acgattttcg ccgcacgcca cacctggccg
    14041 ataatttccg acacccgcag gttgcggtta aagccctgct gggcggtgga acagaattta
    14101 cactccagcg cacaccccac ctgcgaagag acgcagagcg tggcacggtc gtcttccggg
    14161 atatacaccg tttcgacgcg ctgatcgcca acggcgatcg cccatttaat ggtgccgtcg
    14221 gatgaacgct gttcttcaac cacttccggt gcgcggatct ccgccacctc tttcagtttg
    14281 ccgcgcaaca ctttgttgat gtcggtcatc tcatcaaagt tgtcgcagca atagtgatac
    14341 atccacttca tcacctgatc ggcgcggaag ggtttttcac ctaaatcttt aaaaaactcc
    14401 cgcatctgct gacggttgag atccagcagg ttgatttttc catctttcgt ggtgacgttt
    14461 tcaggtgtga ctaattgttc agacatatgc tattccggcc tcgttattac acgttatggc
    14521 ccctggaggg ttgaaaaaag aaacgccccg gtgagcggct gctcgtccgg gggcgctgca
    14581 ttgtacaaat tctggcgcac ggatgccacg tttgcacgcg gcatttacga aattattaac
    14641 gggtgcgcgg acacacttcg ccttcgccga agaaataagc gatttcgcga gcggcagatt
    14701 cgacggaatc agaaccgtgg gtaccgtttt cggtcaggct gtcagcgtaa tcagcgcgca
    14761 gagtaccagc cagtgcgttt gccggattgg tcgcgcccag cagatcgcgg tgacgctgaa
    14821 cggcgttttc accttccagc acggaaacca cgatcgggcc agaggtcatg aattcaacca
    14881 gaccatcaaa gaacggtttt ccatcgtgtt cagcataaaa gccacgtgcc tgttcaacgg
    14941 tcaggtgcag cattttggtg ccgacaattt tgaaccctgc agcttcaaag cgcgcaaaga
    15001 tattaccaat gacgtttttt gctaccgcgt tcggtttgat gatggaaaaa gtacgttcaa
    15061 tagccatttt tacctctgta aattgttctg ttgttgtctg taccagcgta cgagatggcg
    15121 cggattataa tgagcaacag ggccgttgac tattgatgaa ggtaacattt ttttaaaata
    15181 aacccagttt aagcaacaat cagggacaaa acagactatt gcatgacaaa tttcactgtc
    15241 gcgatttgcc ccacgtcatc catcaccagc aattgataat cacctttatc cgtcaaatgc
    15301 agggtcacgt tgcgcccgcg ttcagttaac ggttcgccat tcagaaacca ccagcgttca
    15361 cctgcccctc cacttgattg caacggcaaa gtcgcttccg ctgcgcccgg taaacgttta
    15421 ataatcgctc catcgcgaac gcctgtcagc tgcagcggga gctgggcatc gtgaccgtat
    15481 ggcgggcaaa ttgtcgaggc cggtggcaag cgcacagcgc gacgttctga tgcgggcagc
    15541 cagggttcca gcggtagcgg ccagacattt atcatttctt gtcgcgcttg cgggcaatca
    15601 gcggcaacac gtttgccgtt ttcatccagc cagatgggga aacgaatgcc attaatgcct
    15661 tcctgctccg gcagtaatag agttggcggc tggctcccgt ccagcaacca ggttgccagg
    15721 cggcgccgac agttaccgtc accttccggc aaagactgtc cgcccggcca gcagataacg
    15781 ccacgagtga ctgagttcgg gcgcgggtct tccggcagat tcgcactgcg cgacagtaaa
    15841 atattattga cctgattcaa caatggtacg gcgctggcaa aaccaaactg accaacaacg
    15901 ggcgtgccgt ccggtctgcc agtccagatc ccaatgacat agcgagcgtt aaccccaatc
    15961 gcccaggcat cacgatagcc atagctggtg cccgttttcc atgccagtgg ggcgacgcgc
    16021 ggcaaggcac tatccggtaa gggttgcgct tcatcagcca taatccggcg aatgatccac
    16081 gccgcccccg acgacattaa aggccgttca agcagcggat cgtcaggctg taagcgcaat
    16141 ttgcctgcct tgccgtggcg agcaaacgcg gtatacgctg ccgccatatc ttccagtttt
    16201 gcaccagcgc cgccgagaat gagtgaaaga ttcggcgcag caccgttggg caaatataac
    16261 ggcaatccaa cattgcgtaa ctttgccgca aaccgtttcg gtccataggc ttccagcacc
    16321 tgcacagcag gtaagttcag cgagcgcacc agcgcctcgc tcatgctgat cgggccatga
    16381 aaaccgctat caaagttacc tggtcgataa tcaccggtgc gccgggggac gtcttgcagc
    16441 agtgatgccg ggtggatcaa gccttcatcc agcgccagac cataaacaaa cggtttgagc
    16501 accgatcctg gcgatcggat cgcattgacc atatcaacat gaccaaagcg tgaatcatcg
    16561 ttgagatcta ccgatcccac ccagccgcga acacgcatat cggtatgatc aaccacgatc
    16621 atcgccagtg agctgcgcgg tggtaaacgt cctttccagt tttgcgccag ttcttccaga
    16681 cgtcgttgaa gaccggcatc cagcgtagta acgattttgt cgcttttgct tttaccgagc
    16741 atcatgcgcg aaaacagcgg tgccagttgc ggcatttgtc ggggtgccag ccagatgggt
    16801 tcttcccttg actcttttac ctgcttacgg gaccacacac cttgctcagc catccgttcg
    16861 agcactttat tacgcgcggc ttcggcacgc tccggccaac gatccgggcg cagtcggctg
    16921 ggcgcttgcg gtaacaccgc cagcattgcc gcctcggaat agcttaaatt cgcaggcgat
    16981 tttccgagat aagcccaact tgccgcaccg atcccctgca acgtaccgcc aaacggagcg
    17041 cggttaagat acaaggtcag aatttcacgc ttagacagat gccattccag ttgcaacgcg
    17101 cgccagagct ggcgaatttt gccgccaaat gttttggggt gaggatcaag cagacgagca
    17161 acctgcatag tgagcgtgct gccaccggaa ataacccgtc ccgaagtgag atcttgccat
    17221 gctgcgcgcg ccaccgagaa tggattcacc cccggatgct tccagaacca gcgatcttca
    17281 taattgatca gcgcttcaag gtaacgtgga gaaacatctt cgattgttac cggataacgc
    17341 cagatgccgt cagcatcggc gaagcgccag agcggcgtac catcctgcgc cacgaccact
    17401 cgtgcgggat tgacttcatg cagcggtaga ggccagagtt tatctgctcc ccacgcagca
    17461 agaaaaagga gaaagggcgc agctgccagc gttatccagc agccgcgttt ggttaacaag
    17521 cgaggcattt acggtctgac aatcagcaaa tcttcagccg cgccggtcgc ccgccattgg
    17581 ggaacataca ttgattccac catcggttgc ggtacctgat acgttcccgg cgtcaccgcc
    17641 cgcgccagat acaccagcgt taccggttgg tattcatcaa cggcaaccgc cgccacaaag
    17701 cgatcgtcac ggaactcaat gtgcttaata ctcgcctgct gcatctggtt cagtaagttc
    17761 tgcacttcgc caccactttg ctccaggctg gcgctaccgt tcgccagatt ctggttttcc
    17821 agttccagac ccgcaggcag cagatccacg accaacgcat ccggcacgct gttactggct
    17881 tttacctgca accacaccag caccagatcg ccgctacgta acgagtccag cgatttgctc
    17941 ttaccatcag taccaagaat atgacgctcg atttgcagca cattgttcgc cggtaaaggt
    18001 gcggattgcg gataaccgct ggcatccaca cgcaaccata acggctgatc gccactgttg
    18061 ctcacctgca aggtggcaag ttgatcgcta ttcagattgc tgttttgcgc tttctcgcct
    18121 gtcagcggct cagctgagaa agaggtttgc gcctgccatt tacccggtaa atcctgaatc
    18181 gtacgggcag ccaggaacaa cgcgttactt tcctgcgtcg atagccagcg ttcaccaaac
    18241 gcctgctgcg aaagtgcgtt cagcagggag tactgctcat cgggtagcag tttgttctct
    18301 tccagcaagg agagcattaa cgcgttgtcg cgcagtggac taccgtaatc acccagccat
    18361 atccgctcat cactatttct cggcgttttc agcgccagca caatcgcttc ttcaccacgc
    18421 gttgcatcac ccatggtttt cagcgcaacg ccaagttgca gcagcggtaa accagaagcg
    18481 gcatctgcac gatgctccca gatttcacgc agcgcaccca gcggagcctt ttgctgacgg
    18541 gccaacacca gcgcagcgta agactgtacg gcgaatttac tggctttgag attatccgcg
    18601 tacgggatcg acatcatgcc cggatcttgt aaatagcgca gcagacgctc attaccccgg
    18661 ttaatggcgt ctgtcggcac gctgtaaccc tgttcgcctg cgcggaccag gaaatccatc
    18721 acgtaagccg tcagccagta ctcttcgtca ccgtttttat cccacagcgc aaagccgccg
    18781 ttatcacgtt gcatttgcag caaacgggaa atgccgatat cgaccgatgc acggcgtttc
    18841 tcatcactgt cgcctttgat gcccaacgcc tgcagttggg ctgcgttggt ataaagtgac
    18901 gggaacaggc cgctggcggt ttgctcaaga cagccgtacg gatacgcttt taactctttg
    18961 atataacgtg cgatgttcag cggtggtttg ccgctcaaca acaattgccc ttccagcgta
    19021 acaggcgaga agttttgcaa tccatccgcc ggaatcgccc atgtcccacc aggctgtaac
    19081 gccgtaccgt aattaaccgt ttgtgccggg aacgccggac ggacaccgat tttccactgc
    19141 ttatgctgat cggcaacggt ttcacccggt aacgctaacc cgctaatggt ggcctgaatt
    19201 tctccatcgc cataacccgg caatgctcgc accgggataa acagcgtagt acgcacacct
    19261 ggcgctaatt caacggctgc gggtgaatcg ctgaccagtt caagcaaacc actggcggtc
    19321 agggcaacgt tcagtttttg cggtttatcg gtaagattag tgatatccag cgtcagacgc
    19381 gaggtatcgc cactcgccat aaagcgcggc atgttcagtt cagcaatcac cggtgcggca
    19441 actatcactt tgctttcgtt gctaccgaag tcatctgccg tccaggcttg cgccatgacg
    19501 cgcaattcac cgttaaaatc gccaatcggc agtgtaaccg agccttcgcc ctgttcgttg
    19561 agcgttaccg gcagcgcctg ctgcgcgaca atattgacgt gattgaccgg cggtttacca
    19621 ccacgtttca gctcatcacc atcgccacca aaacgcagag ctgccagacg cccctgacct
    19681 tcaataacct gaccgtaaat atcgtaaatg tctgcgccat agcgtttctg accaaagaac
    19741 gcctgccacg gatctggcgt gacgtagtca gtaatattca gcacaccact atcaacggca
    19801 gacaccagca cattcacctg tttaggcttc tcgccatttt tagtgctggc tttaattttc
    19861 acggttaatg gttgattggg acgcattttt gctggtgttt ccagcgccag atcgaggcga
    19921 cggttttcat caccaagcgg cagatgcaac acaccaaccg cgcgttttgg cgtcgcggag
    19981 cgagatttat cgccaggacg caccaccagc gtactcaaat aaagatcatg acgattccag
    20041 gttttatcga ccggaatcgt cagatccagc ccttgagccg gaacatcaat ctcttgccac
    20101 cacagcggcc cttcactgga ctcgaccatc gcataacctt tacccgccgt tggcacggca
    20161 atatgcaact taatggtatc gccagggcga taactggctt tatccagttt cagcgtgaca
    20221 cggtcgggtc gcacagcacc gctcccgtcg ctgttgtcct gccagctata gccagcccag
    20281 aaacgcacac tactgaccgc ttcattcggc gctttgactt ccagacgata agcgccccac
    20341 tctaccggga aactgacctt accggtttca tccgcctgaa gatccagagt ttgttcattt
    20401 tcgatcagat ctttttgatc aaactgtgac tgccagcctt catcttctga ccagttccag
    20461 tagtaatcgc ggcgttcgcg aatcaggcgc acctgcaagc ccgacacggc ttttttcacg
    20521 ccttgtgcat cgctataaac gatgtcaaaa gcggcgttac tgccttcatc aacaatgggc
    20581 tgtttcaccg tgctgtcagt gcgataatcg taaaccgatt tcgaggcgaa cagcggacgg
    20641 atccccggca atgcatcggc tggccagata gcctgctcag cacggcgcgt caccgggcga
    20701 ccacctgact caagcaaact gccctggaaa ataacctgta atggggaatg cgtttccttc
    20761 cactggcttt ctgttgaaac ttcgccgcgc cctttatcat ccagcgtcaa ctgaacttca
    20821 tccagcgtgc gggaaaggtt ttcggcagct atatcgccga attcaaaacc aggtaaggct
    20881 gacacagcct cacgcagtgg gcgcaggaaa agttgccctt gcaaagtatt accattagca
    20941 ggtgcaccat acaggtagta ccccaccacg gagaatttca cttcatcttt cggcgttagc
    21001 ggggttttct caccggtcag attcagcgcc atgcgctctg gcataaaatc ttcgacgtgg
    21061 aaatcccaca tccgatactg attatcgccc gtgttagcgc gaatatgcca cataccggtt
    21121 gccgcattgc tatcgagtgg ccaggtaaag tggtagaggc cattctccgg ctgactaacg
    21181 acgctcctga gtacctgccc atcgggttta atcacgtcta acttgatggg ttgattgggc
    21241 aacgctttac cgtctgcatc acgcagcaaa ccattgagga ttaccgtttc acctgggcga
    21301 taaagatcgc gtgggccaaa catgaaaaac tgtttgctat agcctggcgc gccagcaatg
    21361 ttaaattctg ctaagtccag cgccggaagt tttaaatcga gtagcgttgt ctgaccgtct
    21421 ttacgcgcca acagtaatgc cgcgttttta tcattttcca gctgcacatg cccctgagcg
    21481 tcactggttg cctgagtcag agtctgccct ttctcattta ataaagagac ttcaattcct
    21541 tgctgggccg cgccgttttc cagactttgg gtaaagatat ccagacgatt gtgataacgg
    21601 tgagctgaaa cgccgatatc acttaacgta aacagcgtcg cgggattact gtaatcgtaa
    21661 cgtccagcct gattcatcac agccagatac acgcccgcct gctgaagcgg tttgatatcg
    21721 cccagcggca gcaataattt ttcacgggtg ttacgcgcag gattgagatc aaaccgtccg
    21781 gtgtagacca gatccgccat ctgcagcagt ttgtctgact gccagttcgc cagcgaattg
    21841 cggtattccc attggctaat gaatgctggc agagattctg gcttaacgcg gaagaagtta
    21901 acatcgacat tattaacgtt gagcgccatt accggcagcc cttcaacgac tttgccaggc
    21961 agcagcgaac cacggctggc aaaaccgacg ctgggttgga tgtcgcgggt agttatagtt
    22021 ttttcgtaat ctttactgaa ggttgcgttg ttgagtgctt tgacctcctt gccaatagta
    22081 acgatcaaat cacgtttcgg ttcgaggtgg cgtaaacgta actctttaag attatctgac
    22141 agctcccagg caccatccac tttgccgctt tttttatcga cgacatgaat aacgcgtgag
    22201 aaatcctgat ccgggtcgag agggatggag aacgtcagca ccagcgtggc tgcaccatca
    22261 agttggactt cagagagatc cagcaatgtc agcgccttac cggcactctg ttgtgccagt
    22321 ttttgtcgct ctggcgcgga gagttttgct gaactcgcgt tttctgaaga ggccgcttta
    22381 gtaacttcag aaggcgcatc ttttttcacc gctgttggcg cgttatcgtt gttgtcgcac
    22441 cctgccagcg ccagcattag catgcaggcg gctacgcgta actttttcat ttttcatccc
    22501 tgccacaatg gcccgttagc aacgtcgaat aattattatg cgtgagaatt tacgttatgt
    22561 aattcagtat cacgcccata caatccgaat tttagtgaga attgtggctc agacagccaa
    22621 atcagcctct tttgcttgtc gcttttcact aaacatccga caatttagcc taaccccggc
    22681 aaaaatggag atgcctatgt ccacgacatg gtttgtagga gccgactggc tcgccgaaca
    22741 tattgatgac ccggaaattc agattatcga tgcccgcatg gcgtcgcctg gacaggagga
    22801 tcgtaacgtt gctcaggagt atctgaatgg acatattccc ggcgcagtgt tttttgatat
    22861 cgaagcgctt tctgatcaca cttccccgct tccgcacatg ctgccgcgcc cggaaacgtt
    22921 cgccgtggcg atgcgtgaat taggcgttaa ccaggataag cacctgattg tctatgacga
    22981 aggtaatctt ttctcagccc cacgagcatg gtggatgctg cgcacctttg gtgtagagaa
    23041 agtgtcgatt ctggggggtg gacttgtagg ctggcagcgc gatgatctgc tgttagaaga
    23101 aggtgcagta gagctgccgg aaggagagtt taacgccgcg tttaatcctg aagccgtggt
    23161 gaaagtaacc gatgtattat tggcaagcca tgaaaatacg gcgcaaatta ttgatgcccg
    23221 cccggctgca cgttttaacg cagaagttga tgaacctcgc ccaggtttac gtcgcggaca
    23281 tattcccgga gcactgaatg ttccgtggac ggaactggtg cgcgaaggcg aactaaaaac
    23341 gaccgatgaa ctggatgcga tattttttgg tcgcggcgtc agctacgaca aaccaattat
    23401 cgtcagctgc ggctctggtg taacggcagc cgtggttttg ttagcactcg cgacgctgga
    23461 tgtgccaaac gtgaaactgt acgacggcgc atggagtgaa tggggcgcgc gggcagattt
    23521 accggttgag ccagtgaaat aagtatttta caggcaataa aaaaccgccg aatttggcgg
    23581 ttttttattg ctagtctggt tcgcggcctt tccagcaggt tgacttgtgt tacatgagca
    23641 acgcaggtgc ttcacagcaa aacaatactc accagtaact ctctttttgt caagcaaaag
    23701 agagtaatta ttgtttattt agcgtattat cgacaccggc cctttccgcc gtgttcggta
    23761 ataaaataac ctggcttatt agtccgaatt cagacaaata taaacaaatc ctgctcaaaa
    23821 ttaaaaattc taaccggtaa aagatattac ttaaacatgt aaattcactt tcctttaaaa
    23881 aacaaaaaac cgccaaaatc aggcggtttt ttgttgctgg tccggttcgc ggcctttcca
    23941 gcaggttgta ttaccgtagt aatgcaagcg cgtctcagcg gagacaatac tcgccagtaa
    24001 ctctcttttt gccaagcaaa agagagttat tattgttctg ttagtgtatt atccactgcg
    24061 gccctttccg ccgtctcgca aacgggcgct ggctttagga aaggatgttc cgtggccgta
    24121 aatgcaggtg tttcacagcg cttgctatcg cggcaatatc gccagtggtg ctgtcgtgat
    24181 gcggtcttcg catggaccgc acaatgaaga tacggtgctt ttgtatcgta cttattgttt
    24241 ctggtgcgct gttaaccgag gtaaataata accggagtct ctccggcgac aatttactgg
    24301 tggttaacaa ccttcagagc agcaagtaag cccgaatgcc gccctttggg cggcatattt
    24361 tagattatcc gattctgttt aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat
    24421 ggcgcaatat gttcggtaat aaagtggcta attccttttt cccctttttt cacctgacaa
    24481 atatcgattg gttcatcgcc aggtaatgta tcggtcgcta cacttcccgt cgcctgaata
    24541 atttcttcga tatcaccatc ggcttcaatg ccaataagta aattaggctg tgcctcttcg
    24601 ttctctttaa ttgaacaaat aaaagcacgc ttcaccggct taatggtttt aaataaggtg
    24661 gtgagtgaat caatcatttg tgctggcggc tctgcgactt ccgataatat cagcgattca
    24721 ccgccttcca ggatttcctg gctgctcagc ggatttccct cttcaccaat caacaaactg
    24781 atttcacgcg gcataaattc tttaccggtt ggcagtttgg cattaaggaa gagcgtttcg
    24841 ccaagtgtca tctcaaacag cgtgcgaacg ggcattacga caaatgcctg ttcgtcttca
    24901 accgcctgtt gaagtgcttc taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct
    24961 ttttcccagt gctgtaaatc aagcgcgcta tcttcaacca cagcctcgcc ctgcgccgcc
    25021 gtaccaggca cccagacggt ggattccagt agagtacgga aaaaggccgg gcggtgcgcc
    25081 ggttcagttg ctgctttttc cagcaggtct tcaagttcgt tttttgtttc ggacataaga
    25141 accacaattc attcaacgtt cggggcgcaa atgcctgatg cgctacgctt atcaggccta
    25201 caagggattc gcaatttgtt gaatttgcag aatttgtagg ccggataagg cgtttacgcc
    25261 gcatccggca tattagttta cgccgttaac agattagcta tcgtgcgcac accaagtccc
    25321 gtagcgcccg cagaccactg ttcaaccggc gctttacggt aagtcgccga gcagtcgata
    25381 tgcagccagc cttgctgata gttctcaaca aagtgcgaca ggaagcccgc cgccgtgctc
    25441 gcgcctgccg gatacgccgc gcttccggta ttgttcagtt cggcaaagtt agacggcagc
    25501 tggctgcggt ggaactccgc cagcggcaga cgccagaacg gttcgttctc ctgcgcggca
    25561 ctcgccagca agcgaccggc cagcgcatcg tcaaaactga acagcgcgtg ataatcatta
    25621 cccagcgcag ttttcgccgc cccggtgagg gtcgccgcat caatgatcat ttccggtttc
    25681 tgcgcactgg catcaatcag accatcggca agcaccagac gcccttcggc atcagtgttc
    25741 atcacttcaa cttttttacc gttgcgatag gtgatgatat cgcccagctt gaacgcattg
    25801 ccgctaatca ggttatccgc acagcagagg aacagcttca cgcgcttgtt cagtccgcgc
    25861 gtaatggcaa atgccagcgc cccggtaacc gttgccgcgc cgcccatgtc cgacttcatc
    25921 gagtccataa acgcagtctg tttgatgctg tagccgccgg agtcaaaagt gataccttta
    25981 cctaccaggc acgcgtacac tggcgcttct ttatcgccag ttgggttgta atccagcgcc
    26041 agcaataccg gagaacgttc tgaaccgcgt ccgacggtgt gcagccccat ataaccttgc
    26101 tcacgcagat cttcgccttt ggtgatccga taagtcacac gatcgcccgc gacgttgctg
    26161 atcagatcaa cagcacgctg tgccagttgc gatggtccca attcttctgc cggtgcgttg
    26221 atggtgtcac gcacccagtc gatgatcatc aggcggttat ccagttcctg gcgctgggca
    26281 tcgtccagat ccggccactc cactttacgc gtgcctttcg gggctttgta accttgccag
    26341 aatgcccagc agcgatccgc atcccaacct tcaccgctta actgaacatg cttgatgccc
    26401 agaccgtcaa tcttgcgggc cgcacgctgg atcagcccca gatcgtctgc cccgttcaaa
    26461 tgcagggtaa tgccatcatt attaatgctg taagttgctt tttctcccca gcgcgcgtcg
    26521 gcaggttggg tagagagggt aatcttcatc gcttctgtca ttttagttat ccttctttgt
    26581 aaaagggccg ccagttggca gccccaggat ttttccgcta aatgattcgc gttgcaggaa
    26641 ggcggcaagt gagtgaagcc ccaggagcat agataactat gtgactgggg tgaacgagcg
    26701 cagccaacgc atctgcggcg tgaagcatga cgcggaaatt attcggcctc gtccagccag
    26761 actaacaaaa tcgcttcgag gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct
    26821 tccagatcgc aaatccactg atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg
    26881 ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat cggtccactt aagtcccata
    26941 ctaacctctg ttaatgctca cgcgcatggt tgatagtgta acgcgggatt tcgactacta
    27001 aatcttcgtc ggtaacgcgc gcctggcagc ttaaacggct ttccggctcc agtccccagg
    27061 ctttgtccag catgtcgtct tcctgctctg agctttccgg cagtgagtca aaaccttcac
    27121 gaacgatgca gtggcaggtg gtgcaagcac aggatttttc acaggcgtgt tcaatctcga
    27181 taccgttacg cagagctgcg tcgagaatgg tttcaccgct attagcttcc agaacagcgc
    27241 catcagggca gagatcctga tgaggcaaaa taacaatctt tggcatatta aacctcgtcc
    27301 acggaatggc ctttcagcgc acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc
    27361 tgggtttgtt tgtctacgtt tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc
    27421 gccacttcac tcaggtgagc ggcagcatcg tcaatgacct gacgttctgc ggcgcttaac
    27481 agcgcggcat cagcagccag cgcgccgtgc agactttcca gcacacgcgc cgcttctact
    27541 ttttgttctg ccagcattcg ggcttttacg tcctgctcgg catagctcat tgagtctttg
    27601 atcatcgaag cgatttcgct atcggtcaga ccgtaagacg gtttgacctg aatagacgcc
    27661 tcaacgccgg tggatttctc catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg
    27721 aacgtcacgc gaatatgcgc accgccagcc ggtagcgccg gaataccacg cagcgcaaaa
    27781 cgcgccagtg agcggcagtc ctgcaccagt tcgcgctcac cctgcattac atggatagac
    27841 atcgccgtct gaccatcttt aaaggtggtg aaatcctgag cgcgggccac cggaatagtg
    27901 gtattacgcg gaatcacttt ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt
    27961 gggatcacat caagcaacag catttcgctg tctggcttgt tacccaccag aatatccgcc
    28021 tgaatcgccg cgccaatagc gacgacttta tccgggtcga tggaagtcag cggtggacga
    28081 ccgaaaaatt cgcctacccg ttcacgcacc agcggcacgc gagtagaacc gcccaccatc
    28141 accacttcca gcacttcatc agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc
    28201 agtaaggttc gttttaccag tggcgcgatc agttcattga attgttcacg gctgatttcg
    28261 ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat cgctcagcgc gattttggct
    28321 gcaatggcgg catccagcag ttcacgctga acgcggttat cgctacgatc aggaatgccc
    28381 gcctgctcgc gaatgtaatc cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa
    28441 tcaccgccgg ttgccagcac ttcaaacacg ccgcgactta agcgcagaat ggaaatatca
    28501 aacgtcccgc caccgaggtc ataaacggcg atcacgcctt cctgaccgga atccagcccg
    28561 taggcgatag ccgcagcggt cggttcgtta agtaagcgca ggacgtgaag gcccgccaga
    28621 cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata
    28681 actacaccat ccagctcgcc tgccagggct tcagttgccc gcgccgccag tgctttgagg
    28741 atgtccgcag aaacgcgcac cgggttcagc agccccgccg ccgtttcaat catcggcagg
    28801 ccgttttcgc tggcctggaa ttgataaggc agatgcggat agcgttgctg gatatcagcc
    28861 agcgagcgtc ccatcaggcg tttaacagaa ctaattgtgt tggcggtatc gagcgctgca
    28921 ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt gatagtgaac aacagatggc
    28981 agcaggtgac ggccttcatg atcggctaac gtttcggcct gaccgctgcg cactgtcgcc
    29041 accagcgagt tggttgtgcc caggtcaata ccggccgcca gacgacgctg atgcggcgca
    29101 gcactcaaac caggttcact aatttgtaat aaggccatgt ttagcttcca gaaattaaaa
    29161 atcgagcagt ttttcttcga gttgttcggc actgcttcgc agtttatcga gaaaacgcag
    29221 cttacgcacg gtatccgccg ccgcgtccca cgtctcgttg tctaactgtt caaccatcaa
    29281 ctgatggcgg gtatcaaaca tctttttcac acgtttgata aagctttcca gccgcgcttc
    29341 atctttcgcc tgttcgatct cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa
    29401 cgcggtgtcg cgcacagtat gctgctcgct ggcgagatca aagccgtgca aagaaagcaa
    29461 atattccgcg cgcattaacg gatgacgcag cgtttgccag gcctggttaa tggttgcaga
    29521 ttgctgtacg gcggcgagtt gttccgcctg gcttccgctg gcgaatttat caggatgata
    29581 ctgacgttgt agatcctgaa aacgcaggct cagcgcctgg gtatcgagtt gatagcgggc
    29641 aggcaagcca aagagggtga agtaatccat aacattctca gggctgcgat ccgccgcgat
    29701 aacggcgaat caggtaaaac aaaccccacg cgcaggcgac cacggtgggg ttatcggtat
    29761 gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat ctttgacgtt cgggttggtg
    29821 aatttaaacc cttcgttcag gccttctttt acgaagtcca gctgcgtacc gtccagaaat
    29881 tgcaggcttt tgccatcgac cacgactttc acgcctttgt cttcaaacac gatgtcttcc
    29941 ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac ctgaacaccc ggaggttctc
    30001 acgcccagac gcaggccaaa ccctttaccg cggttagcca gaaaggtatt tactcgcgct
    30061 gctgcactgt cgctcagtgt aatcgacata accaaacctc aactcttatt ttgcttcacg
    30121 tttgctttta tagtccgcaa tggcggcttt gatcgcgtct tctgccagaa tagaacagtg
    30181 aattttcacc ggcggcagtt caagttcttc agcaatatcg gtgtttttga tcgcctgcgc
    30241 ttcgtcgaga gacttccctt tcacccattc ggtgaccagg gagctggaag cgatagcgga
    30301 accgcagccg taagttttaa aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat
    30361 ctgcaacttc atcacgtcgc cacaggccgg tgcccccacc atgccgctgc cgacgttctc
    30421 gtcgttgttg tcaaaggaac ccacgttacg cggattctcg taatggtcga taactttttc
    30481 gctgtaagcc attataaatt ctcctgattc cgataccgat taatgatgag cccattcgat
    30541 gctgttcaga tccacgccct gcttgtacat ttcccacagc ggagaaaggt cacgcagacg
    30601 accgatggat ttacgaacta actcgatggt gtagtcgatc tcttcttcag tagtaaaacg
    30661 acctaaagag aaacggatag agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag
    30721 cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc
    30781 tttcagcgcc ataatcagcg actcaccttc aacgtagttg aagctgacgt tgagaatgtt
    30841 cggcgcaccg tgttccaggt caccgttcag gtaaacttct tcgatatctt tgatgccgtt
    30901 ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg gtcgccatct cttcttttgc
    30961 gatgcgatag gcctcgccca ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat
    31021 accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg cgtacgcgcg gtttacgacg
    31081 tacatacagc gcaccgatac ctttcgggcc atagattttg tgaccggaga aagacatcag
    31141 gtcaactttc aactggctca ggtcgatagg cagtttaccc acgctctggg ttgcatcaac
    31201 gtgatagata atgccacgag cacggcacat ttcgccgata gccgcgatat cctgcaccac
    31261 gccgatttcg ttatttacgt gcatgatgga cacgaggatg gtgtcgtcac gcatcgctgc
    31321 ttcaagttct ttcaggtcga taatgccgtt acgctgcggt gccaggtagg tgacttcaaa
    31381 accttcgcgc tccagctgac ggcaggtatc cagtaccgct ttgtgttcgg ttttgctggt
    31441 gatgatgtgc ttgccttttt tctgataaaa gttggctgca cctttgatcg ccaggttgtc
    31501 agattcggtt gcaccagagg taaagacgat ttcacgcgga tcagcgccga ccagatcggc
    31561 aatctgatta cgggcgatat ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg
    31621 ggaggccggg ttaccaaagg ttccgtccat cgttataaac tgcatcattt tctcggcaac
    31681 acgcgggtcc accggcgtgg ttgcggagta gtcgagataa atcggtaatt tcattgctct
    31741 ataaactccg tacatcactc aatgcaagga atcaggctac cggctggatg tacgaccgtg
    31801 tttacggagt atttagcact ccggcctgat tctgaattct ttttattaag cgcgtaactt
    31861 aacgtcgatc gcgtcttgtg tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga
    31921 cacatccagc acttcctggt tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc
    31981 ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc aggcatttat cgccgccctg
    32041 gcagccgcct ttaccctgac aacgggtggc atctacagat tcgtcaacgg cgctaattac
    32101 ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg
    32161 tacgctggaa accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag
    32221 ggaaattccc tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag
    32281 cgcaacgtca agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatgtctta
    32341 cttcacctca aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata
    32401 gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt
    32461 cgggtattta accttcagtg ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc
    32521 ggcgtgaacg ccatatccgc cctggggttc tgt
//
LOCUS       DAZFZC010000050        32494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFZC010000050 DAZFZC010000000
VERSION     DAZFZC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.050"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(128..289)
                     /gene="rhuM"
                     /locus_tag="ACHKXP_002712"
                     /pseudo
     CDS             complement(128..289)
                     /gene="rhuM"
                     /locus_tag="ACHKXP_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(368..462)
                     /locus_tag="ACHKXP_002713"
     tRNA            complement(368..462)
                     /locus_tag="ACHKXP_002713"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(426..428),aa:Sec,seq:tca)
     gene            755..2137
                     /gene="yicJ"
                     /locus_tag="ACHKXP_002714"
     CDS             755..2137
                     /gene="yicJ"
                     /locus_tag="ACHKXP_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO7647688.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2147..4465
                     /gene="yicI"
                     /locus_tag="ACHKXP_002715"
     CDS             2147..4465
                     /gene="yicI"
                     /locus_tag="ACHKXP_002715"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO7647689.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4518..6227)
                     /gene="yicH"
                     /locus_tag="ACHKXP_002716"
     CDS             complement(4518..6227)
                     /gene="yicH"
                     /locus_tag="ACHKXP_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7647690.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINTEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(6348..7739)
                     /gene="xanP"
                     /locus_tag="ACHKXP_002717"
     CDS             complement(6348..7739)
                     /gene="xanP"
                     /locus_tag="ACHKXP_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO7647691.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8019..9224
                     /gene="gltS"
                     /locus_tag="ACHKXP_002718"
     CDS             8019..9224
                     /gene="gltS"
                     /locus_tag="ACHKXP_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO7647692.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9393..11474)
                     /gene="recG"
                     /locus_tag="ACHKXP_002719"
     CDS             complement(9393..11474)
                     /gene="recG"
                     /locus_tag="ACHKXP_002719"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO7647693.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11480..12169)
                     /gene="trmH"
                     /locus_tag="ACHKXP_002720"
     CDS             complement(11480..12169)
                     /gene="trmH"
                     /locus_tag="ACHKXP_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO7647694.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12176..14284)
                     /gene="spoT"
                     /locus_tag="ACHKXP_002721"
     CDS             complement(12176..14284)
                     /gene="spoT"
                     /locus_tag="ACHKXP_002721"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO7647695.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14303..14578)
                     /gene="rpoZ"
                     /locus_tag="ACHKXP_002722"
     CDS             complement(14303..14578)
                     /gene="rpoZ"
                     /locus_tag="ACHKXP_002722"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO7647696.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14633..15256)
                     /gene="gmk"
                     /locus_tag="ACHKXP_002723"
     CDS             complement(14633..15256)
                     /gene="gmk"
                     /locus_tag="ACHKXP_002723"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO7647697.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15514..17196
                     /gene="ligB"
                     /locus_tag="ACHKXP_002724"
     CDS             15514..17196
                     /gene="ligB"
                     /locus_tag="ACHKXP_002724"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO7647698.1"
                     /translation="MKVWMAILIGILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGAKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17193..17810)
                     /gene="yicG"
                     /locus_tag="ACHKXP_002725"
     CDS             complement(17193..17810)
                     /gene="yicG"
                     /locus_tag="ACHKXP_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO7647699.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18102..18926)
                     /gene="dinD"
                     /locus_tag="ACHKXP_002726"
     CDS             complement(18102..18926)
                     /gene="dinD"
                     /locus_tag="ACHKXP_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO7647700.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYQGLYGGLDQKAIHQRKGLKKNQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVMQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19147..20010)
                     /gene="yicC"
                     /locus_tag="ACHKXP_002727"
     CDS             complement(19147..20010)
                     /gene="yicC"
                     /locus_tag="ACHKXP_002727"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO7647701.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20137..20853
                     /gene="rph"
                     /locus_tag="ACHKXP_002728"
     CDS             20137..20853
                     /gene="rph"
                     /locus_tag="ACHKXP_002728"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO7647702.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALVDALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            20919..21560
                     /gene="pyrE"
                     /locus_tag="ACHKXP_002729"
     CDS             20919..21560
                     /gene="pyrE"
                     /locus_tag="ACHKXP_002729"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO7647703.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(21597..22193)
                     /gene="slmA"
                     /locus_tag="ACHKXP_002730"
     CDS             complement(21597..22193)
                     /gene="slmA"
                     /locus_tag="ACHKXP_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO7647704.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22300..22758)
                     /gene="dut"
                     /locus_tag="ACHKXP_002731"
     CDS             complement(22300..22758)
                     /gene="dut"
                     /locus_tag="ACHKXP_002731"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO7647705.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(22736..23956)
                     /gene="coaBC"
                     /locus_tag="ACHKXP_002732"
     CDS             complement(22736..23956)
                     /gene="coaBC"
                     /locus_tag="ACHKXP_002732"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO7647706.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24128..24796
                     /gene="radC"
                     /locus_tag="ACHKXP_002733"
     CDS             24128..24796
                     /gene="radC"
                     /locus_tag="ACHKXP_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7647707.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25013..25249
                     /gene="rpmB"
                     /locus_tag="ACHKXP_002734"
     CDS             25013..25249
                     /gene="rpmB"
                     /locus_tag="ACHKXP_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO7647708.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25270..25437
                     /gene="rpmG"
                     /locus_tag="ACHKXP_002735"
     CDS             25270..25437
                     /gene="rpmG"
                     /locus_tag="ACHKXP_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO7647709.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            25535..26344
                     /gene="mutM"
                     /locus_tag="ACHKXP_002736"
     CDS             25535..26344
                     /gene="mutM"
                     /locus_tag="ACHKXP_002736"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO7647710.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(26383..26862)
                     /gene="coaD"
                     /locus_tag="ACHKXP_002737"
     CDS             complement(26383..26862)
                     /gene="coaD"
                     /locus_tag="ACHKXP_002737"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO7647711.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(26870..28147)
                     /gene="waaA"
                     /locus_tag="ACHKXP_002738"
     CDS             complement(26870..28147)
                     /gene="waaA"
                     /locus_tag="ACHKXP_002738"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO7647712.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            28560..29618
                     /gene="rfaQ"
                     /locus_tag="ACHKXP_002739"
     CDS             28560..29618
                     /gene="rfaQ"
                     /locus_tag="ACHKXP_002739"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO7647713.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLIPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            29615..30739
                     /gene="waaG"
                     /locus_tag="ACHKXP_002740"
     CDS             29615..30739
                     /gene="waaG"
                     /locus_tag="ACHKXP_002740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7647714.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYIVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            30732..31529
                     /gene="rfaP"
                     /locus_tag="ACHKXP_002741"
     CDS             30732..31529
                     /gene="rfaP"
                     /locus_tag="ACHKXP_002741"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO7647715.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            31545..>32494
                     /gene="waaO"
                     /locus_tag="ACHKXP_002742"
                     /gene_synonym="rfaI"
     CDS             31545..>32494
                     /gene="waaO"
                     /locus_tag="ACHKXP_002742"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFO7647716.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLDDKAIVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPLMRPVNSNYARYCAKHNF"
BASE COUNT         7731 a         8024 c         8646 g         8093 t
ORIGIN      
        1 tgatggcagt ctgattattg gtgaagttta tagtcagttt ttttcgccag cggttaaaga
       61 aatagaatag tcgtataaaa aagggtgttc tggggcgagt aaaacaacat cttcacctta
      121 atggcgatac agttggctga gagtcagcag gggggcaaat cacctgcaaa taaacacggc
      181 gcatactggc gtgaatatcg cggatgcgtt cgaggacaga agaaccgaca ggcggattct
      241 tcagccgctc atcgtccatc acaaaacctt agaccaggta ttcctgaagc cgattgaata
      301 ttgagcagta gaaaatatct ggattgacag tattacaagg ggttggagaa cgtcaggaga
      361 ggcattttgg cggaagatca caggagtcga acctgcccgg gaccgctggc ggccccaact
      421 ggatttgaag tccagccgcc tcaccggaga cgacgatctt ccgcgcctcg attgctacat
      481 ggaggcgggg cgcattatag ctacttcctt gagtttctac atcccccaga tcagtttttt
      541 ccgcctaata cacgcagcgt attatcacga aagcattata gttcatataa aacccatatt
      601 ttacaatgat atatgagcgg aattaacgct accacgatca cataaaacaa gaatcataaa
      661 ttaataacca gatatcggaa tattcgctct cacagggatg gtttacaaaa tgcgttatcg
      721 gtctcagcac ccctatagca tcaaggaaaa gcagatgaag agtgaagtgt tgtccgttaa
      781 agagaaaatt ggttatggca tgggagacgc cgccagccac attattttcg ataacgtaat
      841 gttatatatg atgttctttt ataccgatat ttttggcatt cctgccggtt ttgtcggaac
      901 catgtttttg gtcgctcgtg cactggatgc gatttccgat ccttgcatgg ggttgttggc
      961 cgatcgaacg cgctctcgct ggggtaaatt tcgtccgtgg gtactgtttg gcgcactgcc
     1021 attcgggatc gtctgtgtac tggcctatag cacgccagat ctcagtatga acggcaaaat
     1081 gatctatgca gcaattactt acaccctact taccttactt tataccgtcg tcaatatccc
     1141 ttactgcgca ttgggtggtg taatcaccaa tgacccgact cagcgtatct cgctgcaatc
     1201 ctggcgtttt gtgctggcga cagcgggagg catgctttct actgttctga tgatgccact
     1261 ggttaattta attggcggtg ataataaacc actcggtttc cagggcggta tcgcggtcct
     1321 ttccgtggtg gcattcatga tgctggcatt ttgtttcttc accactaaag aacgcgttga
     1381 agcaccacct acaacaacgt ctatgcggga agatttacgt gatatctggc aaaacgacca
     1441 gtggcggatt gtcggtttac taaccatttt caatatcctg gcggtgtgcg tacgcggtgg
     1501 ggcgatgatg tattacgtca catggatttt gggcacgccg gaagtgtttg tcgcttttct
     1561 caccacttat tgcgtgggta acctgattgg ttccgcactg gcaaaacctc tgaccgactg
     1621 gaaatgtaaa gtcactatct tctggtggac gaacgccctg ctggcagtga ttagcctcgc
     1681 gatgttcttt gttcccatgc aggccagcat cactatgttt gtcttcatct tcgtgattgg
     1741 tgtgttgcat caactggtga cacctatcca gtgggtaatg atgtccgata ccgtcgacta
     1801 cggcgagtgg tgcaatggta aacgcctgac cgggatcagt tttgctggca cgctgtttgt
     1861 gctcaaactg gggttggcct tcggcggcgc tcttatcggc tggatgctgg cttatggcgg
     1921 atatgatgcg gcagaaaaag cgcagaacag cgccacgatt agcatcatta ttgcgctatt
     1981 cacgattgtt ccggcgatct gttatttgct gagcgcgatt atcgctaaac gctactactc
     2041 actcacgacg cacaatctga aaaccgttat ggaacagctg gctcagggta aacgccgttg
     2101 ccagcaacaa ttcacctctc aagaagtgca gaactaagga acggcaatga aaattagcga
     2161 tggaaactgg ttgattcaac ctggcctcaa tttgattcac ccgcttcagg tgttcgaggt
     2221 tgaacagcag gataatgaaa tggtggtcta tgctgccccc cgtgatgtgc gtgaacgtac
     2281 ctggcagctt gatacgcctt tatttacgtt gcgctttttc tccccacagg aaggtattgt
     2341 cggtgtgcgg attgagcatt ttcagggggc gctgaataac ggtcctcatt atccgctcaa
     2401 tattttgcag gacgtgaagg tcacaatcga aaacacagaa cgttatgctg agtttaaaag
     2461 tggcaactta agcgcgcgtg tcagcaaagg tgagttctgg tcactggatt ttctgcgcaa
     2521 cggcgaacgt attaccggta gtcaggtgaa aaataatggc tacgtgcagg acacgaataa
     2581 tcaacgcaat tatatgtttg agcggcttga tcttggcgtt ggcgaaacag tttacggtct
     2641 gggagagcgc tttactgccc tggtgcgcaa tggccagacg gtagagacct ggaaccggga
     2701 cggcggcaca agtactgaac aggcgtataa aaatatcccg ttctacatga ctaaccgtgg
     2761 ttatggggta ctggtcaatc atccccagtg tgtctctttt gaagtgggat cggagaaagt
     2821 ctccaaagtg cagttcagcg ttgagagtga atatctcgaa tactttgtta tcgacggccc
     2881 gacgccgaaa gcggtacttg atcgttatac ccgctttact ggtcgtccgg cgctgccgcc
     2941 cgcgtggtcc ttcggcctgt ggctaaccac ttcatttacc accaactacg acgaagcgac
     3001 ggtaaacagc tttatcgatg gtatggcgga acgcaatctg ccgctgcatg ttttccactt
     3061 tgactgtttc tggatgaaag ccttccagtg gtgcgatttt gagtgggacc cgctgacttt
     3121 ccctgacccg gaagggatga tccgccgcct gaaagcgaaa ggactgaaaa tctgcgtctg
     3181 gattaacccc tatatcggtc aaaaatcccc cgtctttaaa gagttacaag agaaaggcta
     3241 tttactcaaa cgcccggacg gttcgctatg gcagtgggat aaatggcagc caggtctggc
     3301 gatttatgac tttaccaatc cggatgcctg caaatggtac gccgacaaac tgaaaggtct
     3361 ggtcgcgatg ggcgttgatt gctttaagac cgactttggc gaacgtatcc caactgatgt
     3421 tcagtggttt gacggttccg atccgcagaa aatgcataac cattatgcgt acatctacaa
     3481 cgaactggtg tggaacgtgc tcaaggacac cgttggtgag gaagaagctg tcttgtttgc
     3541 ccgctcggcc tccgtcggtg cgcagaaatt cccggtacac tggggtggcg attgttacgc
     3601 taactacgaa tcaatggcgg aaagcctgcg cggtggtttg tctattggcc tttcaggttt
     3661 tggcttctgg agccacgata tcggcggctt tgaaaatacc gctccggcgc acgtttacaa
     3721 acgctggtgc gcgtttggtt tgctctccag ccatagccgt ttacacggta gcaaatctta
     3781 tcgtgtgccg tgggcctacg atgatgagtc ctgtgatgtg gtgcgcttct tcacgcaact
     3841 gaaatgccgc atgatgccgt atctgtatcg tgaagctgcg cgtgcgaacg cgcggggtac
     3901 gccgatgatg cgggccatga tgatggagtt cccggacgat ccggcttgtg attaccttga
     3961 ccgtcaatac atgttaggcg acaacgtgat ggttgcgccg gtgttcactg aagcgggcga
     4021 tgtgcagttc tacctgccgg aaggtcgctg gacacacctg tggcacaacg atgaactcga
     4081 cggtagtcgc tggcataaac agcagcacgg cttcctgagt ctgcccgttt atgtgcgtga
     4141 taacactcta ctggcgctgg gcaacaacga tcaacgtccc gattacgtgt ggcacgaagg
     4201 cacggcattc cacctcttta atctgcaaga cgggcatgaa gccgtctgtg aagtgcccgc
     4261 tgctgacgga tcggtgatct ttactttaaa agcagcacgt actggcaaca cgattactgt
     4321 gactggtgcg ggcgaggcga agaactggac actgtgcctg cgcaatgttg tgaaagtaaa
     4381 tggtctgcaa gacggttcgc aggctgaaag tgagcagggg ctggtggtga agcctcaagg
     4441 gaatgcgctg acaattacgt tgtaatctat tgatggaatg ccgggtacgt ctgagtcgta
     4501 cccggcgaga ttgatgttta ccgcaccggc tccgtcgatg taacttcacc gctggaaacg
     4561 atgccagcat tcatggtttg agtcacttgc tgctttgcgg cgttcacggc atgcaattgc
     4621 ccgctaaccg taggtttcaa cgggacattg gcctgaatat cgccactggc ggtaagctga
     4681 atattgccgt cgccagtcaa cggtaattca ggccatcccc actgttgcaa aatattcacc
     4741 ggcacaccgc gtccattcag gctgatatgt gtctgacgtt gtggcgtttg tgaaacactg
     4801 gcggtggctt ccagaatgcc tttttcagta aatgcactca gttcgctgat attcaccgtg
     4861 ctgctgttgg cggtcagcgc cagcgacggg cgacgaacat caacacgatt gaatgtcgcg
     4921 gcggcggcat tcagattcgc cgagccactc cagacgcccc atttatgatc ggtaaccagc
     4981 gtcaggttgg caccgtaacc atcgagcgcg gtgagctgcc acgggaagtc agggtcgata
     5041 tcaatgatca gattgcggct ggcgctaaat ctcttcagtt gcaggctgtt taaccaaccg
     5101 ggtgtcgttt ccatccacaa ctgttgccag tttttcggca aggtatattc cagcccggca
     5161 attgccgcat catcaaggat caacgttttc ccgtcacgca gccagttccc tgacgttctg
     5221 accatacccc cttcccagcg gctggtgaac tggcgcagcg ctacgccctg cggggaaaat
     5281 tccgtgttta taatcgggtc aaataaatgc agcgaaccat aaatgaactc gctagcgttc
     5341 atcgacagtt tgccttcctg tgtctgccag tcatctttac tgaaggtcat gttgcgcaag
     5401 ctgagatcga ggtcggtcac cgcccagtcc ggaccttgca aacgagcatc gatcacttcc
     5461 aggcgaccaa tctgcaacga cgggacagag cgtaatggcg caaagaagtc ggttagcgat
     5521 ttttcgcttt gtagacgtat atccgccatg cgcaggtttt ccacttgcca gctgccgtcg
     5581 gcgttacgct gcgcgtttcc ggttaatgtc ccgcgggcga tgtcggcacc caggttagtc
     5641 agcgtaacgc gatcgttatc aatactgcct tcaatcagta cattggtggc aggaacatca
     5701 ttgagcgaaa gcgatccggc actaaactga atctgcgcct tcgtacccag cactttaccg
     5761 gcttttggtg accacggaac tacgccgcca tttacccgct gcgcgctcag tttccattcg
     5821 ctattcgggc tattaaacgc catatcacgc agttgcagac gatcggcttt gaacggtagc
     5881 ggcgcggtct ggtcggtgag attcagcgtc ccgttttcca gcaggatggt atcgacatgg
     5941 cgtggttcgg tcagttgccg actgcttagc gcaatgtcga cacttttggc caccagggtc
     6001 gcgggctggc catcacgacc aaacgtgacg ttctccagca cgatatgaga tggcgcggaa
     6061 aaacggtgat ccatcgcccc gaaggccaga tgatagtcgc tattctcgga aacccatgcg
     6121 ctgatatgtt ctgctcccca gcgggtttgc agaagaaaat agaggccagc gatcgccact
     6181 aacagagcga tgagaatgta gagaagcagc ttcccaataa atttcatggt cttccatccc
     6241 gtgaaatgca cataagggag ttatgcacga tttacgcgca atcctcaagg cgggaatggt
     6301 taaagaggtg agactgcggc ggtaattatc ttaccgccgc cagtgaatta ctgtttttct
     6361 ggtgggaaaa tcagattcag cacgatggca gtaataccgc ccgcggcgat cccggaggag
     6421 agcaggtttt tcagccattc aggggcaaac tgcaaaatca gcggctgctg agacacgccc
     6481 agaccaaccg ccagcgacag cgcgataatc agaatcgccc gacggttcag cggctcacga
     6541 gaaacgatac gcacaccgga ggcggcgatg gtgccaaaca ttacaagcgt tgcgccgccc
     6601 agaaccggtt ctggaatgtg ttgtacaaaa ccgctcactg ccgggaacag acccagcacg
     6661 atcaacatca gcgcgacgac aaaaccgaca tagcggctgg caacaccagt caactggatc
     6721 actccgttgt tttgcccgaa gcaggagttc gggaaggtgt taaacaccgc cgaaacaaac
     6781 gagttcaggc cgtttgccag cacgccgcct ttcaggcgtt tcatgtacag cggaccggac
     6841 actggctgtt cggaaacgtc agaggtcgcc gtgatatcgc caatggtttc cagcgaagtg
     6901 atcataaaga ccagcatcag cggcagcagc agactccatt caatgccaag accgtaatag
     6961 agcggcgttg gcaccataat cagttcttgc gtcataggtt cgttgctttc tggcaacatg
     7021 cccataaacc acgccagcgc atatccggcc gccatcgcaa ttaccagtga ggccacgcgt
     7081 aagtaagggt tacgttgacg gttaagcagg ataattaagg ctaagaccac gcctgccagc
     7141 agcagatttt tcggtgcgcc gaaggtgtta tcgctcatgg ctgcgtaacc gccgccaatg
     7201 gacgttaacc caacctgaat tagcgacagg ccgataatca tcaccacaac gccagaaacc
     7261 agcggcgtaa taatgcggcg cgccagatgc agaacgcggg agatcaccat ctcggtgcaa
     7321 cttgccagca tcaacgtgcc gaacaaagcc gccatcatgg taggaacatc agcaccaccg
     7381 gttttcagcg cggtaccgcc cataatcagc ggggcaacaa agttgaagct ggtgccctga
     7441 atagacaaca gcccggagcc aaccggaccc caggccttaa tttgaataat cgatgccaca
     7501 ccggaggcaa acagcgacat actaataatg tgttgcgtgt cttgtgccgg taaacccagc
     7561 gcctggcaga ttaatagcgc tggcgtgatc accgcaacga acatcgccag cagatgctga
     7621 caggcggcaa acagggtttg aggaagcggc ggacgatctt caagacggta aatcagttcg
     7681 ctgttttgag tctgcgcaac cggttgcgca ttttctgact cgagggtgga aacagacatc
     7741 gaaaacaatc ccgtggtagc aaagcgggca ttttagctga ccgaaagaga aaagcaaacg
     7801 tttgccacat cctcatctaa atatttaaat gagataaatg cactttttta tataactttt
     7861 ggtatttttc tgcttaaaat ccatgccatt tgttcattaa cagctgagaa tttgacccag
     7921 gaaagcataa ctcgataccc gtcatagttc atgaagttgc cactgcgcga tgaagtatga
     7981 cgagtatgaa agagtgatgc ggatacaaag gagtaactat gtttcatctc gatactttag
     8041 caacgcttgt tgccgcaacg ctgacgttgc tgctcgggcg taagttggtc cattccgtct
     8101 cctttttgaa gaaatacacc ataccggaac ctgttgcggg tggtttgttg gtggcgctgg
     8161 cgctactagt actgaaaaaa agcatgggct gggaagtcaa ctttgatatg tccctgcgcg
     8221 atccgttaat gctggctttc ttcgccacca ttggcctgaa cgccaacatt gccagtttgc
     8281 gtgccggtgg gcgtgtggtt ggcatcttct tgattgtggt tgttggtctg ttggtgatgc
     8341 aaaatgccat tggcattggt atggctagct tgttagggct tgatccgctg atggggctgt
     8401 tggccggttc tattacgctt tccggcggtc acggtacggg cgctgcgtgg agtaaattgt
     8461 tcattgaacg ttatggcttc accaatgcga cggaagtggc gatggcctgt gcaacgttcg
     8521 gtctggtgct gggcggcttg attggcggtc cggtggcgcg ctatctggtg aaacactcca
     8581 ccacgccgaa cggtattccg gatgaccagg aagtcccgac ggcgtttgaa aagccggatg
     8641 tgggacgcat gatcacctcg ttggtgctga ttgaaactat cgcgctgatt gctatctgcc
     8701 tgacggtggg gaaaattgtt gcgcaacttt tggctggcac tgcttttgaa ctgccgacct
     8761 tcgtctgtgt actgtttgtt ggcgtgattc tgagcaacgg tctgtcaata atgggctttt
     8821 accgcgtctt tgagcgtgcg gtatccgtgc tgggtaacgt aagcttgtcg ttgttcctgg
     8881 cgatggcgtt gatggggctg aaactgtggg agctggcttc gctggcgctg ccgatgctgg
     8941 cgattctggt ggtacagacc atcttcatgg cgttgtatgc catcttcgtt acctggcgca
     9001 tgatgggcaa aaactacgat gcggcagtgc tggctgcggg tcactgtggt tttggcctcg
     9061 gtgcaacgcc aacggcaatc gccaacatgc aggcgatcac tgaacgcttt ggcccgtcgc
     9121 acatggcgtt tttggtggtg ccgatggtcg gtgcgttctt tatcgatatc gtcaatgcgc
     9181 tggtaattaa gttgtatttg atgttgccga tttttgccgg ttaaccgatg aagcggcggt
     9241 agaagtgccg ccgcaacaaa gacaaatgcc tgatacgctt cgcttatcaa gcctgcgtat
     9301 tgattcataa tttattgaat ttgtagggtg atgaatcgca tccggcagga aggtagggta
     9361 acctgaaatg gcggtcttct cactgccgcc ttttacgcat tcgagtaacg ttccgtctcc
     9421 ggcatccagc gttctatcag ggcttttgcc tgttgtgggt aacgttcgtg aatatggcgt
     9481 gccaggcgct gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc cactttaaat
     9541 tcagcattac ccgtctgacg cgtgcctaac aattcgccag ggccgcgaat ctccagatct
     9601 ttttgcgcaa tcacaaaacc gtcgttactg tcgcgcagca cttgcaggcg aatttgcgcc
     9661 gttttagaaa gcggcgtttt gtagagcagc acgcagtgag aagccaccgc gccacgacct
     9721 acgcgcccgc gcagctggtg taactgcgcc agacccagac gctccgggtt ttcgataatc
     9781 atcagactgg cgttaggcac atcaacgccg acttcaataa cggttgtggc aaccagcagg
     9841 tgtagctcac cttgtttaaa cgacgccatc accgcctgtt tctcggcagg tttcatccgc
     9901 ccgtgtacca ggccaacgtt caactctggt agcgccagtt tcaactcttc ccaggtagct
     9961 tccgccgcct gcgcttccag caattccgac tcttcaatca acgtacaaac ccagtatgcc
    10021 tgacgacctt cagttatgca ggcgtggtgc acgcggtcaa tgatgtcggt acggcgggta
    10081 tcaggaatag cgaccgtagt cactggcgtg cggcctggcg gcagctcatc tatcaccgag
    10141 gtatcgagat cggcatacgc agtcattgcc agcgtgcggg ggatcggcgt ggcggtcatg
    10201 atcaactgat gcggatggaa gccctgctgc tggcctttct cccacaatgc cagacgctga
    10261 tgcacgccaa aacgatgctg ttcgtcgata atcaccagcg ccaggccgtt aaactgcacc
    10321 tgttcctgga agatggcgtg tgtaccgaca atcatctgca cctgaccgct ggcgatggct
    10381 tcctgctgtg ccagccgtgc tttacctttc tgcttaccgg cgagccagcc cacttcgata
    10441 ccgagcggtg caaaccagtt gcggaagtta ttggcgtgct gctcggcgag taattcggtt
    10501 ggtgccatca atgctacctg tttgccgtgg gcaatcgcac gcaacgcggc gagggcggcg
    10561 accagcgttt taccggaacc tacatcgccc tgcaccagac gcatcatcgg cacatccagc
    10621 gccatatcgc gctcgatctc cgccactacg cgtgcctgtg cgcccgttgg cttgaacggt
    10681 aaggcggcga ggagtttatt tttcagcgtg tcattggcgc tcagcggctg ggcatgaaaa
    10741 cgctgtgctc cggcacgtaa ggctaacatg ctgaggttgt gcgccagcag ttcttccaga
    10801 atcagacgac gttgcgccgg atgctgcccg gtttccagat cgctaagctg tagcgtcggt
    10861 ggcgggcggt gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg tgacagttcc
    10921 ggcggcagga gttcttcaat ggcgcaggtg tcgagcagat ccagcgcctg gtcggttaat
    10981 ttacgcagcg tggcctgctt tacgccttcc gttgttggat aaaccggcgt gagcgtttcc
    11041 tgtaattctg gcgtgctgag atcgccctgc acgcggtatt ccgggtggat catctccgca
    11101 ccatatttac cgcgctttgc ttcgccataa gccagtacac ggcggcccgc cgccaggcta
    11161 tttttcattg ccgcgctgaa attgaaaaag cgcatggtga ggatgccgga accgtcgctg
    11221 atctggcagg tcatcatccg ccgaccgccg aaggagatat tgcagttcag cacttcgcct
    11281 tccaccgtgg cataaacgcc cggcagtagt tctccgatgg ggtagagatg ggtgcgatct
    11341 tcgtagcgca ggggaaggtg taagagtaga tcctgcacgg tatgcaggtt gattttcgcc
    11401 agtttgttac taagtgctgc gccaacgccc gttagggaac tgagtgggac agcatctaac
    11461 aggcgacctt tcatggcact taccctgcag cctgcatagt agcccaccag tcggcatcag
    11521 cttcgatctc gccttgctga ttgacgtggg gataaggcag gcctttgcgt tttgcgactt
    11581 tcgccagcac cggatagccg ccttcaaaca acaggcgttg ttgctctgct tccggcaaca
    11641 tgctgttttc acgcaggtac atgcctgcat tttgccgctg acgctgggct tcgtaaagaa
    11701 tgagggctga ggcaacggaa acattcagcg actgcaccat gccgatcatc ggaatgatga
    11761 tgtcctgatc cgccagggcc aatgcttcct gcgtgatgcc cgttttctcc tgtcccatca
    11821 aaatgcaggt cgggcgagtg taatcaattt cgcggaaatc gacagcgtta tcagaaagat
    11881 gggttgccag aatctgcatg ccctggcctt tgagatgagc gacggcatcg ccaatggtgc
    11941 ggtgtgtttt cacctgtacc cagctgttac tacccgccgc tgccgaagcc atggtgcgca
    12001 tgcggctacc aggccagacg gcgtgaactt catgtacgcc aacggcatct gcggtacgaa
    12061 taatcgcaga aacgttatga ggtttgtgga cctgctccat gcagacggtc agatcaggct
    12121 gccgcctggc gagcatttcg cagatgcgtg cataacgtgt tgggttcata aaacattaat
    12181 ttcggtttcg ggtgacttta atcacgtctg gcatcacgcg gattttgcgc atgatattcg
    12241 ccagatgcac acggtcacga gcggtcagac gaataaaggc gctgtagacg cgaccatctt
    12301 tctcttccgt attcaaactt tgaatattcg aagtcgtggt gttaattgcc gccgtcaggt
    12361 ttgccagcgc accctgatga ttgaacatct ccaccttgat ttcggtgatg aactcctgcg
    12421 ccgtctcttt atcccattcc acagccataa acttctctgg ctctttctgg tagccacgga
    12481 tattacggca ggattcatgg tggatcacca gacctttacc ggggctgacg tgggcgataa
    12541 tcgggtcgcc aggaataggg cggcagcatt tcgcaaaggt gatcagcacg ccatcggcac
    12601 ctttaatggg cagatgtccg tggctttggg ttgccggtgg aatggaggcg tccccatgtt
    12661 gcagattttt cgcgaccacc acgctcattg cgttaccaag tccgatttct gccagcagat
    12721 cgtcaagcgt tgccagcttc atgcgatcca gctcgcgctg aatattttcc tgcgggattt
    12781 cattcagctt acggctacca cccaaagcat ggttgagcag acgacggccc aggcttacag
    12841 aatcatcacg cttgaggttt ttcagcaact gacgaatttt ggcgcgcgct ttcgagctaa
    12901 cgacaaagtt cagccaagcg gcattcgggc gagcgcccgg agcggtaatg atttcaacgg
    12961 tttgaccgct ggtaagcggc tgcgacagcg ggtaaggctg gcggtcaacg cgtgcgccca
    13021 cgcaggcatg accgatatcg gtatgcactg cataagcgaa gtcgacgggc gttgcaccgg
    13081 caggcagctc gacaatgcgc ccttccggtg tgaaaacgta aatctcatcc gggaagagat
    13141 cggatttaac gctctcgata aattcaaacg aactaccggc gctctgttgc agctccagca
    13201 ggctttgcat ccagcgctgg gcgcggattt gtgcggtagt actggtttcg ccgtgctctt
    13261 tataagccca gtgcgcggca acacccatct ccgccatctg gtccatatct tcggtacgga
    13321 tctggacctc aaccggcaca ccgtgcgggc cgatcatcga ggtgtgcaaa gactgatagc
    13381 cgttcgcttt tggaatggcg atatagtctt tcacgcggcc cggacgcggc ttgtacaggc
    13441 tgtgcatctg gcccagcacg cgataacagg tgtcagaatc attgacgatc acgcggaaag
    13501 cgtagatgtc catgatcgag tgaaaacgct gctctttgag caccattttg cagtaaatcg
    13561 aataaagatg cttctcgcga ccactgacgc ggcacggtat tcccgcttcc tgcaaacgcc
    13621 cttcgatttc agaaagaatc ttctggatca tctctttacg gttgccgcgc gcggctttca
    13681 ccacttcttt gattacgcga taacggttgg gatacagcgc ctcaaaaccc agctcttcga
    13741 gttcggtttt aatgtggtgg atacctaaac ggtgcgccag cgggctataa atttcgagag
    13801 tttcacgggc gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg cgcatgttgt
    13861 gggtacggtc ggcaagtttg atgaggatga cgcggatatc ctgcaccatc gccataatca
    13921 tcttgcgaaa gttttcggcc tgcgcctctt tcttatcgcg gaacttgagt ttatcaagtt
    13981 tcgacacccc ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc atatcctggt
    14041 aggtggcggg agtatcttca atcacgtcat gcagcagcgc cgccatcagc gtttcatagt
    14101 cgagtttcat ctcggccaga atgcaggcaa ccgctaccgg gtgcgtgata tagggttcac
    14161 cgcttgaacg tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc tgccgcagac
    14221 gcttgatttg gtcttccggc aggtaggttt gaatcagttg attcaggctt tcaaacagat
    14281 acaagggcga cccgctttgt gattaacgac gaccttcagc aatagcggta acggcttgta
    14341 attcagcggc ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg atctggttgt
    14401 tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg ttttcttccg
    14461 gtaccagcgg atcctttccg cctacctgca tctgacgagc gcgacgcgcg gcgaccagta
    14521 ccaggtcaaa acggttacca attttctcta cagcgtcctg aacagttacg cgtgccatac
    14581 ttaaaaagct ccacaggtga agaaatgact gggcatgata ctgaaatcag gttcagtctg
    14641 ccaacaattt gctgattaaa gcgtcatgac gctgcttttg gcggctcatg cgcagacgtt
    14701 cggcgcgaat aatggtcttc aaatcggtca acgcggtatc gaagtcatca ttcacaatca
    14761 gataatcata ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata cgctttgcaa
    14821 tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg gtccagttca attttggacg
    14881 gcggtaaaat aaagatactc cgcgcgtgcg gcatcttctg gcgaatttgc tgcgcgccct
    14941 gccagtcgat atcgagaaaa acatcgacac cggtcgccag tacttgctca atggcctcac
    15001 gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca tctctgctaa
    15061 tcatttcttt aaattcatca tgattaacaa agaaataatg ttcaccgtgg acttcaccag
    15121 gacgcggttg gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac ggttgggttt
    15181 ttaataaagc ctgaatcagg ctggatttac ccgcgccact gggggcagaa acaatataaa
    15241 gcgtgccttg agccatgagt atctttcgta tgtgattagc gaaataaagc ctacatacga
    15301 gcttattata cacggcgcgg ccatgtgacg tagcccttgc tcacactttt tgcgcaaagt
    15361 tgttgcgttt tgcgtagctg cttccgcatt tgcctgtcat tcactgcaat aaagacgatt
    15421 ttcaggaagc gtcctcgcag actccgtttt tgccactcgg ctttgctttc atcacgttgt
    15481 atatctgcct gactatctac agcggaggtg atgatgaaag tatggatggc gatattaata
    15541 ggtatcttgt gctggcaatc atctgtgtgg gcggtctgtc cggcctggtc gccagccaga
    15601 gcacaggaag aaatttcccg cctgcaacag caaataaaac agtgggacga tgactactgg
    15661 aaggaaggaa aaagtgaggt ggaagacggt gtttacgatc agttaagcgc ccgtcttacg
    15721 cagtggcaac gctgctttgg gagcgagccc cgcgacgtga tgatgccgcc tttaaatggt
    15781 gcggttatgc atccggttgc ccatacgggt gtacgcaaaa tggtggataa aaacgcatta
    15841 agtctgtgga tgcgagaacg tagcgatctt tgggtgcagc caaaagttga tggcgttgcg
    15901 gtaaccctgg tttatcggga cgggaaactg aacaaagcaa tcagtcgcgg taatggcctg
    15961 aaaggcgagg actggacgca gaaagttagc ttaatttccg ctgtgccgca aaccgttagc
    16021 gggcctttag ccaacagtac gcttcagggg gaaatatttc tccagcgcga ggggcatatc
    16081 caacaacaaa tggggggaat aaatgcccgc gcaaaagttg ctggcttgat gatgcgccag
    16141 gacgatagcg acacgctgaa ttctctgggc gtttttgtct gggcatggcc ggatggaccg
    16201 cagttaatgt ctgatcgttt aaaagagctg gctaccgcag gttttactct aacgcagacg
    16261 tatacccgtg cggtgaaaaa tgctgatgaa gttgcgcgcg tacgcaatga gtggtggaaa
    16321 gcggaattac ccttcgtcac cgatggcgta gttgtacgag cggcgaaaga gccagaatcc
    16381 cgccattggc taccgggcca ggcagagtgg ctggtggcct ggaaatatca acctgtagct
    16441 caggttgccg aagtgaaggc aattcagttt gcggtgggta agagcggtaa aatatcggtg
    16501 gttgcgtcac tcgcacctgt catgctggat gataaaaaag tccagcgggt gaatattggt
    16561 tccgtcaggc gctggcagga gtgggatatt gcgcctggtg atcagattct cgtcagcctt
    16621 gccggtcagg gtattcctcg cattgatgat gtggtgtggc gcggtgcaga acgtacaaaa
    16681 ccgacaccgc cagaaaaccg ctttaactcg ttgacctgct actttgcttc tgatgtttgt
    16741 caggaacagt tcatttcacg cttagtctgg ctgggggcaa aacaggttct tgggctggat
    16801 ggcattggtg aggccggttg gcgcgcgctg catcagactc atcgctttga acatatcttt
    16861 tcctggcttt tattaacgcc agagcaatta cagaacacgc cggggatcgc gaaaagtaaa
    16921 agtgcgcagc tatggcatca gtttaatctg gctcgtaagc agccttttac tcgctgggtg
    16981 atggcaatgg gaataccgct aacccgggcg gcgcttaatg ccagtgatga acggtcctgg
    17041 tcgcaacttt tatttagcac ggagcagttc tggcagcagc tgccggggac tggatcggga
    17101 cgcgccagac aggttattga atggaaggaa aatgcgcaaa tcaagaagct gggcagttgg
    17161 ctggctgccc agcagatcac aggttttgaa ccttagtgtc cttcgtggct gtagtaaaag
    17221 accggtaatc ccagcttaag gcgtagcgcc agtaagcggg caaaaaagcc gaataccagg
    17281 gtggagataa ttaccacatc atggttagaa acatagtgtt gcagcgcgat gtacagcacg
    17341 gcggaggcaa atgagacacc ggcatacaac tctttctgga ataccagtgg gatacgttta
    17401 cagaacatat cgcgcaaaac gccgccaaac acgccggtgg ttaccgccgc gacaacggca
    17461 ataattgggc cgtgccccat atccagtgca acctgtgcgc cgatgataga aaagaccacc
    17521 agtccaagcg catcgagcac cagaaacact ttgcgcaggt aaggcattac aggagcaacg
    17581 atagtggtga gtactgcggc ggtagcgacg ataatcacat attccgggtg tttgacccag
    17641 ccgagcggat agtggcccag cagaatatcg cgcactgacc ctccgccaat tgcggtggcg
    17701 gtcgcaataa taattacgcc aaatgtatcc atgcggcgtc gcccggccgc cagagccccc
    17761 gtcatggctt cggcagtaat gccaaccaaa tacaaaatgt gtaacagcat ggttctctcc
    17821 ctaaaaatct gtctggcagg gtagcgattt gtgcgtattg tcacgattga gattttttaa
    17881 ggtgagagga aattgataac ttgtagttat gtgaaatcgc tattttctgt agcgtagatg
    17941 cattcttctg acttcttgtc tgagaaaatg cataagaaaa aataggttaa aattgagggg
    18001 tggaaaagaa aggtgcaatg tttagccatt gtgttcaccg cacctggact ttggcttacg
    18061 catcgggtat ctcaatcaac aaaaagagtt tttagtttct gctatttctt ctctgtaatt
    18121 tttacactgt tttccaactg cttgatgctg acctgcgggg tcggcaactc ctcaggcatg
    18181 gttccgccaa gttcctgaat ggtttgcatc accttgctac ccacgtcaaa gtgtgtggta
    18241 ttagcttgct gttttgaatt cacgccatcc cgcttgagtt tttcttctgt ttgggtagct
    18301 cgaaagagat tagccgccag ttctgttgaa cccatatgat ccaggatctt ctgattcttt
    18361 ttcagcccct tccgctgatg aatagctttc tgatctaatc caccatacag cccctggtaa
    18421 ccatgattct gaaatatggc gaaatcaata gctgtcgcta ccccagcttg ctgtgctgcc
    18481 tcaaccaatt gtttgttatg ttctttcaac tcatttctca gaaacagacg cttttcgtct
    18541 tcacgaagtt gcttaaatgc ttcatcgtca gcaagctcct gccgtcgggt ctggatagca
    18601 aaataagtct gccctgccgc aatgaccggt ttcgcagggt cgccgttttg tactaccagg
    18661 taacaggcat agcgggagag tacgatgtct tttaatgctc ttttggcatt tgagccaatt
    18721 ttgaccatat cgaggatctc ctcgaaatgg tcatcaattg tgtgaccact gttctcacag
    18781 gcttctttgg cgcgcgttaa tacaggtata aagtgacgat actctgaata acccagtagt
    18841 ttcccaagtt gtcttgctga ccattgttct gctccgtttg cattaatcag ttttatctct
    18901 tcaaaaggtt gatgatgttc gttcattgcc cactccatgt gatatcaatt gcaaagaaag
    18961 taaatctgta actgtattta tatacagttg tgagtatctc acgttctcag tgtgctgggc
    19021 aaaaaaacat cgcaaaatct ggagcaggca cgcaaaacag aattgaatgt tgcggggaag
    19081 aaaaatgccg ctctgtttga gtggcggcat ttgtgaaggc ggaaaagtaa gaattggcgt
    19141 tacgagttat tcgatgttct gaatctgctc gcgcatctgc tcaatcaaca ctttcagctc
    19201 gatggcggag tttgtcactt cggcattgat agacttcgac gcaagagtgt tcgactcgcg
    19261 gttgaactcc tgcatcataa aatccagacg acgaccaacc gcttcttttt tcttcagaat
    19321 gttgtaggtc tctttgacat gcgcttcgag gcgatccagt tcttcggcaa cgtcaattcg
    19381 ttgtgccagc agaaccagtt cctgctccag acggttgttt tccagttgca cctgagcatc
    19441 ttccagcttc gcgaccagac gctcacgctg ccattgcagg atttccggca tatgggagcg
    19501 gactttgacc acttcggcgg tgacgccttc cagacgctgc tcgatcaatg ctttcagtgc
    19561 ctgaccttcg gtttcgcgcg cgacaataaa gtcgtccagc gtaccatcca gcgccgcgag
    19621 aatttcagcg gcaatggcgt caagatcctg ctcctgggct gccatcacgc ccggccagcg
    19681 tagaatatca accgggttga tttccccttc gtcactctgc atttttaccc agttcgcggc
    19741 agttaccagc tgtttagcca gtttttcgtt gaggatcagc tcaccttgcg cgctaacatc
    19801 tggctcatag cgcagggtac attccacttt accgcgcgtc aggcgagaac gaatacgctc
    19861 gcgaacgaca ggttcaaggc tacggaactg ctccggcaga cgaaagtaag tttccagata
    19921 acgctggttt accgagcgca tttcccaggt tgcgctcccc cattcaccct tgatttcacg
    19981 ccgggcgtag gcggtcatac tgcggatcat agacgttcct gtttataaaa ggagaggtgg
    20041 aaggattata gccatcgatg ccttgtcagg ataggaataa ccgccggaag tccgtataat
    20101 gcgcagccac atttgtttca agccggagat ttcaatatgc gtccagcagg ccgtagcaat
    20161 aatcaggtgc gtcccgttac cctgactcgt aactatacaa aacatgcaga aggctcggtg
    20221 ctggtcgaat ttggcgatac caaagtgttg tgtaccgcct ctattgaaga aggcgtgccg
    20281 cgcttcctga aaggtcaggg ccagggctgg atcaccgcag agtacggcat gctgccacgt
    20341 tctacccaca cccgtaacgc tcgtgaagcg gcgaaaggta agcagggtgg acgcacaatg
    20401 gaaatccagc gtctgatcgc ccgtgctctt cgcgcggcag tagatttgaa agcgctgggt
    20461 gagttcacca ttacgctgga ctgcgacgtg cttcaggctg atggtggcac gcgtaccgcg
    20521 tcgattacgg gtgcctgcgt ggcgctggta gatgcgctac agaagctggt ggaaaacggc
    20581 aagctgaaaa ccaatccgat gaaagggatg gtagccgcag tttctgtcgg aattgtgaac
    20641 ggcgaagcgg tttgcgatct ggaatacgtt gaagactctg ccgcagagac cgacatgaac
    20701 gtagtgatga ccgaagacgg gcgcatcatt gaagtgcagg ggacggcaga aggcgagccg
    20761 ttcacccatg aagagctact caccttgttg gctctggccc gagggggaat cgaatccatt
    20821 gtagcgacgc agaaggcggc gctggcaaac tgatttttaa ggcgactgat gagtcgcctt
    20881 ttttttgtct gtagaaaagt aagatgagga gcgaaggcat gaaaccatat cagcgccagt
    20941 ttattgaatt tgcgcttagc aagcaggtgt taaagtttgg cgagtttacg ctgaaatccg
    21001 ggcgcaaaag cccctatttc ttcaacgccg ggctgtttaa taccgggcgc gatctggcac
    21061 tgttaggccg tttttacgct gaagcgttgg tggattccgg cattgagttc gatctgctgt
    21121 ttggccctgc ttacaaaggg atcccgattg ccaccacaac cgccgtggcg ttggcggagc
    21181 atcatgacct ggacctgccg tactgcttta accgcaaaga ggcaaaagac cacggtgaag
    21241 gcggcaatct ggttggtagc gcgttacaag gacgcgtaat gctggtagat gatgtgatca
    21301 ccgccggaac ggcgattcgt gagtcgatgg aaattattca ggccaatggc gcgacgcttg
    21361 ctggcgtgtt gatttcgctc gatcgtcagg aacgggggcg cggcgagatt tcggcgattc
    21421 aggaagttga gcgtgattac aactgcaaag tgatctctat catcaccctg aaagatctga
    21481 ttgcttacct ggaagagaag ccggaaatgg cggaacatct ggcggcggtt aaggcctatc
    21541 gcgaagagtt tggcgtttaa agaaactcgc cggatgaaaa ttcatccggc gtcatattac
    21601 tgcaactgcg cggcaattag cggccagcgg gcgtcaaaat catccgtcgg gcggtattta
    21661 aattcgctgc ggacaaaacg tgacagcata ccttcacaga aggccaggat ctggcttgcc
    21721 agcagggttt catcggtggt gtaaccttca ccctcacgca ttctcttttc acgcaatacc
    21781 tggcgcagct gcgcttcaat acgctcgaac agctggttga tgcgcccttg caggcgatcc
    21841 tgttcaaaca ttagcgcatg accagtgagg atgcgggtca ggccaggatt acgctcacca
    21901 aaaccgagaa gcagcaacac aatcagacgc aggcgcgctg tggtgtcttt ctcatctttc
    21961 agaatcagat tgatgcgagt aatcaggcta tcttcgataa actcaatcag gctatcgaac
    22021 atgcgggtct tactggggaa gtggcgatac agtgccgctt cggaaacgcc gacagaggcg
    22081 gccagttttg ccgtcgtgat acgttggctt ccatcgctgg attccagcat cagcgccaga
    22141 gactgaagta tttcctcgcg acggttcctt ttcgcagttt gtttttctgc catgttacaa
    22201 aatacccctg aaaataagca cttgccaggc ggcacccacg ctatgaccgc aaacgaaatg
    22261 tttgcggcta tgttatgacg ttattcggat gcgtatgtgt tattgacgac cagagtgacc
    22321 aaagccgcct tcaccgcggt cggtggcgtc gaaatcttcc accagattaa attcagcctg
    22381 tactaccgga acaaaaatca tctgggcgat gcgttcgcca ggttgaatgg tgaagctgtc
    22441 ctgaccacgg ttccacacgg aaatcatcaa ctggccctga tagtcggaat cgatcaatcc
    22501 taccaggtta ccaagcacga taccgtgctt atgtcccaat ccggagcgcg gcagcatcat
    22561 tgccgccagt gaaggatcgg caatatgaat cgccagcccg gtcggaacca gcgtagtgtc
    22621 acccggagcc agttctacgg cgtcgtcgag acaggcacgc aggtcaagtc cggcagagcc
    22681 agaggtggca taagtcggga gcggaaattc cttcccaacg cgcgggtcca gaatcttaac
    22741 gtcgattttt ttcatcataa cgggtcacga tctcgtcgag taataattgg ccaaggagct
    22801 ctttgcgctc aagcggtaag actttatctc cgtcctgcca gaaaaggtgt aatgcgttgt
    22861 tgtcgctgtt aaatccttga gttggctggg aaacatcgtt cgcgcagatc agatcaaggt
    22921 ttttacggat acgtttttgc cgggcgtatt cttccacatt atttgtttcg gcggcaaatc
    22981 caacgacgta gggtcgatgg tcttttagtg cggcaacgcc tgcgacgata tcggggtttt
    23041 taaccatttt tattgttaat tcatcaccct gcgtggcctg ctttttgatt ttttctgggg
    23101 ccacggtagc tgcgcgataa tccgccacgg cggcgcagcc gataaaaata ttttgctgct
    23161 gtacagaagc attcacggcg gcttccattt ccagcgcggt catcacatca acacgtttaa
    23221 caaacggtgg cgtcggtagt gaaaccggac ctgataccag cgtgacgttc gcgccacgac
    23281 gggcagcggc ggcggcgatt gcaaaaccca tcttgccgga gctgtgatta gagatataac
    23341 gcacgggatc gagcggttca cgcgtcggac cggcggtaat cataatgttc agatgtttca
    23401 ggtcgttgac gggcgaaaaa tgcgctaccg ccatatccac aatggttaac ggatcgagca
    23461 ttcgccctgg gccgatatca ccacaagcct gactgccact gtctggcccc cagatgagca
    23521 aaccacggga agcaagcacc tctaaattat gctgcgtggc agcggcacgg tacatctgct
    23581 ggttcatggc ggggagcacg gctacaggcg caggtgtagc cagacaaatc gtcgatacca
    23641 ggtcattcgc cattccggca gcaacacggg caatcaaatc tgccgtggca ggggcgagaa
    23701 tcactaaatc agcccattta cccagctcaa tatggcccat agcggcttcg gctgccgggt
    23761 ccagcagact gtcggaaacg ggataaccag aaaccgcctg caagctaagt ggggtgataa
    23821 aggcttttgc cgcttcggtc atggctacgc ggacgtcggc cccgcgatcg cgcaaacgac
    23881 gcaccagttc aggggtttta taggcagcaa taccgccgct aacgccgaga acgatttttt
    23941 taccggccag gctcatgatg atttttcctg tagggtgaca ccagaagttg gcgattttat
    24001 cacaatactt ttgttgtcgt gccttcacct gagattcact ttgcgaggcg ctttccagga
    24061 ttgaaaactg gccgtcgatt taacgaaacg gctatgacag gatgcgagca ccacaaagga
    24121 ggtgaaggtg aaaaacaatt cacagctgtt gatgccgcgc gaaaaaatgc tgaagtttgg
    24181 tattagcgcc ttaacggatg tcgagctgct ggcgctattt ctgcgtaccg gaacgcgcgg
    24241 taaagatgta ttaaccctgg caaaagagat gctggagaat ttcggctctc tttatggctt
    24301 gttaacctct gaatatgaac aatttagtgg tgttcatgga attggcgtag cgaaatttgc
    24361 ccagttaaag gggattgctg aactggcgcg acgttactac aacgtgcgga tgcgtgaaga
    24421 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
    24481 ggagcgggag atctttatgg tgatctttct cgactcccaa caccgggtta taacgcatag
    24541 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccgcga
    24601 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcatcctt cgggttgtgc
    24661 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
    24721 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
    24781 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
    24841 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
    24901 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
    24961 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
    25021 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
    25081 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
    25141 gaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
    25201 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
    25261 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
    25321 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
    25381 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
    25441 tcgctttgat gtaacgaaaa acctcgctcc ggcggggttt tttgttatct gcttaccccc
    25501 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
    25561 cagccgccgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg ctgtggtgcg
    25621 caacggacgc ttgcgctggc cggtttcaga agagatctac cgattaagcg atcaaccagt
    25681 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
    25741 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgagaa
    25801 gcatgaccat gttgatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
    25861 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggcccatct
    25921 tggaccggag ccgcttagcg acgatttcaa cggtgagtac ctgcatcaga agtgcgcgaa
    25981 gaaaaaaacg gcgatcaaac cgtggctgat ggataacaag ctggtggtag gcgtagggaa
    26041 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
    26101 actgtcgctg gcagagtgtg aattgctggc tcgggtgatt aaagcggtgt tgctgcgttc
    26161 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
    26221 tttcgcgcag gaattgcagg tttacgggcg aaaaggcgag ccctgtcggg tgtgcggtac
    26281 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
    26341 gtaattcatg cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccat
    26401 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
    26461 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
    26521 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
    26581 cgccaccgca cgcagaccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
    26641 catcaaatca ctaaacccga ccacttccac gttccccaga tgcgcggttg cctgctgtgc
    26701 cagctcaaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
    26761 cagaataacg tgatcgaaca tctgcgtggc gcgcgtcacg atatcgatat gaccattggt
    26821 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
    26881 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
    26941 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
    27001 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
    27061 ccagccgcgc gcaaatgtct ttaaagttaa aagtatgcgg ccccatcaat accggaattg
    27121 cgtgtgcggc agcttccagc ggattatgcc caccacgttc aaccagtgaa ccgccaacaa
    27181 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
    27241 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
    27301 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
    27361 ataaattcgg gaattgctgt aacaatgcct gatgtgcggc gatcaccaca ctctcttcgc
    27421 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccattgg cggcgcagcg
    27481 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
    27541 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
    27601 gggcggcaat cagcgtaata cgacgcagca agcgacggac gaatttaccc agtttggcat
    27661 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
    27721 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttag
    27781 ggtcgacttt attcaggaaa cggttgagtg catcgggcag atcatacggc agataaacgt
    27841 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
    27901 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
    27961 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
    28021 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
    28081 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
    28141 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
    28201 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
    28261 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
    28321 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attatatttg attgatttct
    28381 ttattgaaaa tcaaaaatgt gcttaaaaat cattcgggtc ggattgccgc gaaagaagtg
    28441 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
    28501 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
    28561 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
    28621 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
    28681 tgctttatca ggacaccatc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
    28741 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
    28801 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
    28861 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
    28921 agtctgcttt ttggcgtaaa agtttcaccc atttggtgcc gttgcagggt ggaaatgtgg
    28981 tggaaagtaa cttatccgtg ctgataccat tgggacttga ttcgttggtg aagcagacaa
    29041 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
    29101 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
    29161 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
    29221 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
    29281 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
    29341 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
    29401 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccctggtcaa
    29461 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
    29521 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
    29581 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
    29641 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
    29701 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
    29761 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
    29821 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
    29881 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtagcgca
    29941 ggaaaaaggc tttttctatc gcctgacgtc acgttatcgc cattatgccg cctttgagcg
    30001 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
    30061 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
    30121 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
    30181 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
    30241 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
    30301 attgctatat attgttgggc aggataaacc gcgaaaattt gaggcgctgg cagaaaaacg
    30361 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
    30421 ggcggcggat ttattactgc atcctgccta ccaggaagcg gcgggaattg tgctgctgga
    30481 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
    30541 tgtcgaggcc aattgcggcg aggctattgt tgagccattc cgccaggaaa cattgaatga
    30601 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
    30661 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
    30721 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
    30781 cttttgagga agttaaaacc ttgcagggtg aggtatttcg tgaactggaa actcgccgta
    30841 ctctgcgctt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
    30901 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
    30961 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
    31021 ttggcgaaaa aggcatgaat ccgctgacca gaacttcatt tattattacc gaagatctga
    31081 caccaaccat aagtctggaa gattactgtg ctgactgggc gactaaccct ccagatgttc
    31141 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgcgg
    31201 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
    31261 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
    31321 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
    31381 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgccgcc ccgcttaaag
    31441 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
    31501 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
    31561 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
    31621 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggcg
    31681 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
    31741 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
    31801 cctgtatcca gatccatcta gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
    31861 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
    31921 ataagatcct ttacctagat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
    31981 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
    32041 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
    32101 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
    32161 tgcttgatga taaagccatc gtttcccgtt taacctatat ggatcaagat atccttaatc
    32221 ttatcctgtt agggaaagtt aaattcattg atgctaaata caatacgcaa tttagtttaa
    32281 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
    32341 atgtcggccc gacaaaacca tggcattact gggccggtta tccaagtgcg caacctttta
    32401 tcaatgccaa agaagcatcg ccctggaaaa atgaaccgtt aatgcggcca gttaactcaa
    32461 actatgctcg ttattgcgcc aagcataatt ttaa
//
LOCUS       DAZFZC010000051        32422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFZC010000051 DAZFZC010000000
VERSION     DAZFZC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.051"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..1874)
                     /gene="ycaO"
                     /locus_tag="ACHKXP_002743"
     CDS             complement(114..1874)
                     /gene="ycaO"
                     /locus_tag="ACHKXP_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO7647717.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            2004..2696
                     /locus_tag="ACHKXP_002744"
     CDS             2004..2696
                     /locus_tag="ACHKXP_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO7647718.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2895..3983
                     /gene="serC"
                     /locus_tag="ACHKXP_002745"
     CDS             2895..3983
                     /gene="serC"
                     /locus_tag="ACHKXP_002745"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO7647719.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4054..5337
                     /gene="aroA"
                     /locus_tag="ACHKXP_002746"
     CDS             4054..5337
                     /gene="aroA"
                     /locus_tag="ACHKXP_002746"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO7647720.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTGLGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5506..6270
                     /gene="ycaL"
                     /locus_tag="ACHKXP_002747"
     CDS             5506..6270
                     /gene="ycaL"
                     /locus_tag="ACHKXP_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO7647721.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6443..7126
                     /gene="cmk"
                     /locus_tag="ACHKXP_002748"
     CDS             6443..7126
                     /gene="cmk"
                     /locus_tag="ACHKXP_002748"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO7647722.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7237..8910
                     /gene="rpsA"
                     /locus_tag="ACHKXP_002749"
     CDS             7237..8910
                     /gene="rpsA"
                     /locus_tag="ACHKXP_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO7647723.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9070..9354
                     /gene="ihfB"
                     /locus_tag="ACHKXP_002750"
     CDS             9070..9354
                     /gene="ihfB"
                     /locus_tag="ACHKXP_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO7647724.1"
                     /translation="MTKSELIERLATQQSHIPVKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9562..11826
                     /locus_tag="ACHKXP_002751"
     CDS             9562..11826
                     /locus_tag="ACHKXP_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO7647725.1"
                     /translation="MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQLQGYQWIDTPHQGQTTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11863..13611
                     /gene="msbA"
                     /locus_tag="ACHKXP_002752"
     CDS             11863..13611
                     /gene="msbA"
                     /locus_tag="ACHKXP_002752"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO7647726.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13608..14594
                     /gene="lpxK"
                     /locus_tag="ACHKXP_002753"
     CDS             13608..14594
                     /gene="lpxK"
                     /locus_tag="ACHKXP_002753"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO7647727.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTLLASGN"
     gene            14631..15863
                     /gene="ycaQ"
                     /locus_tag="ACHKXP_002754"
     CDS             14631..15863
                     /gene="ycaQ"
                     /locus_tag="ACHKXP_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO7647728.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHDDLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGCC
                     PQGLFTDCRTGWEIDPVA"
     gene            15915..16097
                     /gene="ycaR"
                     /locus_tag="ACHKXP_002755"
     CDS             15915..16097
                     /gene="ycaR"
                     /locus_tag="ACHKXP_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO7647729.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16094..16840
                     /gene="kdsB"
                     /locus_tag="ACHKXP_002756"
     CDS             16094..16840
                     /gene="kdsB"
                     /locus_tag="ACHKXP_002756"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO7647730.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16994..17887
                     /locus_tag="ACHKXP_002757"
     CDS             16994..17887
                     /locus_tag="ACHKXP_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO7647731.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17864..18643)
                     /gene="elyC"
                     /locus_tag="ACHKXP_002758"
     CDS             complement(17864..18643)
                     /gene="elyC"
                     /locus_tag="ACHKXP_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO7647732.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18779..19564
                     /gene="cmoM"
                     /locus_tag="ACHKXP_002759"
     CDS             18779..19564
                     /gene="cmoM"
                     /locus_tag="ACHKXP_002759"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO7647733.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19561..20883
                     /gene="mukF"
                     /locus_tag="ACHKXP_002760"
     CDS             19561..20883
                     /gene="mukF"
                     /locus_tag="ACHKXP_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO7647734.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20864..21568
                     /gene="mukE"
                     /locus_tag="ACHKXP_002761"
     CDS             20864..21568
                     /gene="mukE"
                     /locus_tag="ACHKXP_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO7647735.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21568..26028
                     /gene="mukB"
                     /locus_tag="ACHKXP_002762"
     CDS             21568..26028
                     /gene="mukB"
                     /locus_tag="ACHKXP_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO7647736.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            26289..28136
                     /gene="ldtD"
                     /locus_tag="ACHKXP_002763"
     CDS             26289..28136
                     /gene="ldtD"
                     /locus_tag="ACHKXP_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7647737.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLCDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28317..28865
                     /locus_tag="ACHKXP_002764"
     CDS             28317..28865
                     /locus_tag="ACHKXP_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO7647738.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28892..29539
                     /gene="gloC"
                     /locus_tag="ACHKXP_002765"
     CDS             28892..29539
                     /gene="gloC"
                     /locus_tag="ACHKXP_002765"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO7647739.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29761..30951)
                     /gene="aspC"
                     /locus_tag="ACHKXP_002766"
     CDS             complement(29761..30951)
                     /gene="aspC"
                     /locus_tag="ACHKXP_002766"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO7647740.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31136..32224)
                     /gene="ompF"
                     /locus_tag="ACHKXP_002767"
     CDS             complement(31136..32224)
                     /gene="ompF"
                     /locus_tag="ACHKXP_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO7647741.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7900 a         7887 c         8717 g         7918 t
ORIGIN      
        1 ataattacaa ttattttatt aatgcaaata tatgtaaagc ggggcattaa aaaaacgccc
       61 cgtaatataa ctcagactaa tcattaagcc tacattgcgt aggctatttg attttatttt
      121 gcccagaatg ctgctttggc gcgctgcagc ttttcgtagg ccttcaacaa cgactgatgt
      181 gcagcaaacg cgtgcagatc gctatctacc ggttgcaggc cgtaaaacgc cgcttcgccg
      241 ctcattgccg cactggcggc ttctacggca tctgcgccgt acatgcgaac aaacgcattc
      301 agatattgca gcggctggcg atcttcttcc tgtgccagta ataacaacgt ttgcaggcag
      361 cgataatagt tggcgcgttc cggactaaat actgatgagt taaactccat cgtccattcg
      421 gtccagacca gagcctgttc cagatcgcca ccagccagcg ccagcatggc ttttaattca
      481 ccgatacgca gggtgtacca accgttatcc gacccggtcg ccagacccaa cagctcacgc
      541 acgcgggtaa agtcatcaaa accttcttca tccagttgct cgatgaggtt caggtaatct
      601 tctttttccc actcgctgcc tggtagcgaa agaatcgttt cacgtaaatg gctgcccata
      661 ctgttattcg cgagccacag atcttcagcc ggataaatat cggacatgcc aggcacgata
      721 atacggcaag cataaacgcc cagatgctcg taatcggcaa tataaacttc tttatcttct
      781 ttgttgaaga tagccatcag tgtggcgaac tcttcttccg tggtgccgga gaaattccag
      841 tccacaaacg gataatcggc atcctgcttg aacaggtccc aggagattaa accgctggaa
      901 tcgataaagt gcgtttcgag gttggtatgt tcagcgactt cttcatcatc gaaggttggc
      961 ggagtaaaca catccaaatc tttcaggcca cgaccttgca gcagctcggt cacggtacgt
     1021 tccagtgcta cgccaaaatc aggatgcgca ccgaaagagg caaagcaggt accgttagca
     1081 ggattgaaca gtaccacgca aatcaccgga tactggccgc caagcgaacc gtcataagcg
     1141 aagattggga aaccctccgc ttccagcgtt tcgatggctt caactactgc tgggtaacgc
     1201 gccagcacgt ctgccgggat ctctggcagg ctgatgcttt cagcaataat gcggtttttc
     1261 acgtagcgtt cgaaaacttc agacaatccc tgaacgcgtg cttcgttgcg ggtattacct
     1321 gcggacatac cgttggagac gtacaggtta ccaatgatat tcatcggaat ataaacggtc
     1381 tgattgtcgg actgacgcgt aaacggcagg ccgcaaatac cacgatcttc gttaccggat
     1441 tgtaggtcaa tcagcatgct gccggtcagt tcattctccg gatcataaaa cgcgcgcaga
     1501 cggtcatcga gcagcccttc tggcacatcg tcattttcgg tcagtgggaa ccatttttcg
     1561 ttgggataat gcacgaacgg accgttggcg atggtttcgc ccagccagaa gtccgcaaaa
     1621 aagtagttgg ttgagagacg ctcgaaatat tcaccgagtg cagaagccag cgccgctttc
     1681 ttggttgcgc ctttaccgtt ggtaaaacac agtgcgcact ctttgtcgcg aatatgtaca
     1741 gaccagacgt taggcacggg attcagccag gaggcctctt caatctgaaa gccgaggtct
     1801 gaaagttttt gctggaagcg agcgatggaa tcttccagag cggcatcttt gccggggata
     1861 aatgtttgcg tcatgaaaat cactttagtc gtacggaaag cgcgcaataa tacgggtttt
     1921 atctcaaagg cgctatcacc gccgccatgc cgatgaactg ttgactatgc ttttagcgga
     1981 taaaccacgt taagagcata aaaatgaagg cgttcgatct ccaccgtatg gcatttgata
     2041 aagtgccttt tgatttcctt ggcgaagttg cactacgtag tctttatacc tttgtactgg
     2101 tctttttgtt cctcaaaatg accggaagac gcggtgtgcg gcagatgtcg ctgtttgaag
     2161 ttttaatcat tctgacgctg ggatcagcgg cgggagatgt ggcgttttat gatgatgtgc
     2221 cgatggtccc ggtacttatc gtctttatta ctctggcgtt gttataccgc ctggtaatgt
     2281 ggttgatggc gcacagtgaa aaactggaag atcttctgga aggcaagcca gttgtcatta
     2341 ttgaagatgg cgagctggcc tggtcgaaac tcaataactc caacatgacg gaatttgagt
     2401 tctttatgga gctacgattg cgtggcgtgg agcagctggg gcaggtacgt ctggcgattc
     2461 tcgaaaccaa cgggcaaatc agtgtctatt tctttgaaga tgacaaggtg aaaccgggtt
     2521 tacttatttt acccagtgat tgtacgcagc gttacaaagt ggtgccggag tcggcggact
     2581 atgcctgtat tcgttgtagt gaaatcattc atatgaaagc gggggaaaaa caattatgtc
     2641 cgcgctgtgc aaatccagaa tggacgaagg caagtcgggc aaaacgggtg acctgacagt
     2701 aaaaacatcg gctttttgct aataatccga gagattcttt tgtgtgatgc aagccacatt
     2761 tttgccctca acggttttac tcattgcgat gtgtgtcact gaatgataaa accgatagcc
     2821 acaggaataa tgtattacct gtggtcgcaa tcgattgacc gcgggttaat agcaacgcaa
     2881 cgtggtgagg ggaaatggct caaatcttca attttagttc tggtccggca atgctaccgg
     2941 cagaggtgct taaacaggct caacaggaac tgcgcgactg gaacggtctt ggtacgtcgg
     3001 tgatggaagt gagtcaccgt ggcaaagagt tcattcaggt tgcagaggaa gccgagaagg
     3061 attttcgcga tcttcttaat gtcccctcca actacaaggt attattctgc catggcggtg
     3121 gtcgcggtca gtttgctgcg gtaccgctga atattctcgg tgataaaacc accgcagatt
     3181 atgttgatgc cggttactgg gcggcaagtg ccattaaaga agcgaaaaaa tactgcacgc
     3241 ctaatgtctt tgacgccaaa gtgactgttg atggtctgcg cgcggttaag ccaatgcgtg
     3301 aatggcaact ctctgataat gcggcttata tgcattactg cccgaatgaa accatcgacg
     3361 gtatcgccat cgacgagacg ccagacttcg gcaaagatgt ggtggtcgcc gccgacttct
     3421 cttcaaccat tctttcccgt ccgattgacg tcagccgtta tggtgtaatt tacgctggcg
     3481 cgcagaaaaa tatcggcccg gctggcctga caatcgtcat cgttcgtgaa gatttgctgg
     3541 gcaaagcgaa tatcgcgtgt ccgtcgattc tggattattc catcctcaac gataacggct
     3601 ccatgtttaa cacgccgccg acatttgcct ggtatctatc tggtctggtc tttaaatggc
     3661 tgaaagcgaa cggcggtgta gctgaaatgg ataaaatcaa tcagcaaaaa gcagaactgc
     3721 tatatggggt gattgataac agcgatttct accgcaatga cgtggcgaaa gctaaccgtt
     3781 cgcggatgaa cgtgccgttc cagttggcgg acagtgcgct tgacaaattg ttccttgaag
     3841 agtcttttgc tgctggcctt catgcactga aaggtcaccg tgtggtcggc ggaatgcgcg
     3901 cgtctattta taacgccatg ccgctggaag gcgttaaagc gctgacagac ttcatggttg
     3961 agttcgaacg ccgtcacggc taatgccgaa attttgctta atccccacag ccagcctgtg
     4021 gggtttttat ttctgttgta gagagttgag ttcatggaat ccctgacgtt acaacccatc
     4081 gctcgtgtcg atggcactat taatctgccc ggttccaaga gcgtttctaa ccgcgcttta
     4141 ttgctggcgg cattagcaca cggcaaaaca gtattaacca atctgctgga tagcgatgac
     4201 gtgcgccata tgctgaatgc attaacaggg ttaggggtaa gctatacgct ttcagccgat
     4261 cgtacgcgtt gcgaaattat cggtaacggc ggtccattac acgcagaagg tgccctggag
     4321 ttgttcctcg gtaacgccgg aacggcaatg cgtccgctgg cggcagctct ttgtctgggt
     4381 agcaatgata ttgtgctgac cggtgagccg cgtatgaaag aacgcccgat tggtcatctg
     4441 gtggatgctc tgcgcctggg cggggcgaag atcacttacc tggaacaaga aaattatccg
     4501 ccgttgcgtt tacagggcgg ctttaccggc ggcaacgttg acgttgatgg ctccgtttcc
     4561 agccaattcc tcaccgcact gttaatgact gcgcctcttg cgccggaaga tacggtgatt
     4621 cgtattaaag gcgatctggt ttctaaacct tatatcgaca tcacactcaa tctgatgaag
     4681 acgtttggtg ttgaaattga aaatcagcac tatcaacaat ttgtcgtaaa aggcgggcag
     4741 tcttatcagt ctccgggtac ttatttggtc gaaggcgatg catcttcggc ttcttacttt
     4801 ctggcagcag cagcaatcaa aggcggcact gtaaaagtga ccggtattgg acgtaacagt
     4861 atgcagggtg atattcgctt tgctgatgtg ctggaaaaaa tgggcgcgac catttgctgg
     4921 ggcgatgatt atatttcctg cacgcgtggt gaactgaacg ctattgatat ggatatgaac
     4981 catattcccg atgcggcgat gaccattgcc acggcggcgt tatttgcaaa aggcaccacc
     5041 acgctgcgca atatctataa ctggcgtgtt aaagaaaccg atcgcctgtt tgcgatggca
     5101 acagaactgc gtaaagtcgg tgcggaagta gaagaggggc acgattacat tcgtatcact
     5161 ccaccggaaa aactgaactt tgccgagatc gcgacataca atgatcaccg gatggcgatg
     5221 tgtttctcgc tggtggcgtt gtcagataca ccagtgacga ttcttgatcc caaatgcacg
     5281 gccaaaacat ttccggatta tttcgagcag ctggcgcgga ttagccaggc agcctgaatg
     5341 aacaacgggc aataaatagc caaatctttc tttatcaaaa cgtcggcaca ttgtcggcgt
     5401 tttttttcgg accttgtgag tcattttgat taatggtagc gtcgcttgtc aatttaagtt
     5461 gttgatacat aatatttata tatgattaat caacggatga ttcacatgaa gaatactaaa
     5521 ttactgctgg cgattgcgac ctctgcagca ttactgacag ggtgtcaaaa tacccacggt
     5581 attgatacca atatggctat cagctccggt ttaaatgcct ataaagcagc aacattaagc
     5641 gatgccgatg caaaagcgat tgccaatcag ggctgtgccg aaatggacag cggcaatcaa
     5701 gtcgcaagta aatccagcaa gtacggtaaa cgtctggcaa aaatcgccaa agcattgggt
     5761 aacaatatta atggcacgcc ggtcaactat aaggtttata tgaccagcga cgtcaacgca
     5821 tgggcgatgg cgaacggctg tgttcgtgtc tacagtggcc tgatggacat gatgaatgac
     5881 aacgaaattg aaggcgttct gggccatgaa ctgggccacg tcgcgttggg tcactcgctg
     5941 gctgaaatga aagcttctta tgcgatcgtt gccgcacgcg atgccatttc agctaccagc
     6001 ggtgtggctt cccagctttc ccgctcacaa ttaggtgata tcgcagaagg cgctatcaat
     6061 gctaaatact cccgtgataa agagtccgaa gcagatgatt tctcctttga tctgttgaag
     6121 aaacgtggca tcagcaccca ggggctggtt ggcagctttg aaaaactggc tagcctggat
     6181 ggcggtcgca cccagtccat gtttgactct cacccaccat caacagagcg tgcgcaacac
     6241 atccgtgatc gtatcgcctc tggtaagtaa atcattgtca tctttcgggc tggtcttctg
     6301 ccagcccgct ataattgcgc aataaatccc catctgaata cagacaaaac tggtttttgc
     6361 acacaacgtt aacgatttgt ggcgtcggcg cgtataatgc gcgcggttat gttaacggta
     6421 cgcctgtttt aaggagataa agatgacggc aattgccccg gttattacca ttgatggccc
     6481 aagcggtgca gggaaaggca ccttgtgtaa ggctatggcg gaagcgttgc aatggcatct
     6541 gctggactcg ggtgcaattt atcgcgtact ggcattggcg gcattacatc accatgttga
     6601 tgttgcgtcg gaagatgcgc tggtaccgct ggcatcccat ctggatgtac gttttgtgtc
     6661 gaccaatggc aatctggaag tgatcctcga aggggaagat gtcagcggcg aaattcgtac
     6721 tcaggaagtg gcgaatgcag cttcacaagt cgcggcattc ccacgcgttc gtgaagcatt
     6781 attgcgtcgc caacgcgcgt ttcgcgaatt accaggtctg attgccgatg gccgcgacat
     6841 gggaacggtg gtattccctg atgcaccagt gaaaattttc cttgacgcct cctcggaaga
     6901 acgtgcgcat cgccgcatgc tacagttgca ggagaagggc tttagtgtta actttgagcg
     6961 ccttttggcc gagatcaaag aacgcgacga ccgcgatcgt aaccgagcgg tagcgccact
     7021 ggttccggca gccgatgctt tagtgttgga ttccaccacc ttaagcattg agcaagtgat
     7081 tgaaaaagcg ctacaatacg cgcgccagaa attggctctc gcataagcga ccgaatttgc
     7141 agtacccccg ttgcaatgga atgactgcgg gtatgttaaa caaccccatc cggcatggag
     7201 ccaggtggac gttaaatata aacctgaaga ttaaacatga ctgaatcttt tgctcaactc
     7261 tttgaagagt ccttaaaaga aatcgaaacc cgcccgggtt ctatcgttcg tggcgttgtt
     7321 gttgctatcg acaaagacgt agtactggtt gacgctggtc tgaaatctga gtccgccatc
     7381 ccggctgagc agttcaaaaa cgcccagggc gagctggaaa tccaggtagg tgacgaagtt
     7441 gacgttgctc tggacgcagt agaagacggc ttcggtgaaa ctctgctgtc ccgtgagaaa
     7501 gctaaacgtc acgaagcctg gatcacgctg gaaaaagctt acgaagatgc tgaaactgtt
     7561 accggtgtta tcaacggcaa agttaagggc ggcttcactg ttgagctgaa cggtattcgt
     7621 gcgttcctgc caggttctct ggtagacgtt cgtccggtgc gtgacactct gcacctggaa
     7681 ggcaaagagc ttgaatttaa agtaatcaag ctggatcaga agcgcaacaa cgttgttgtt
     7741 tctcgtcgtg ccgttatcga atccgaaaac agcgcagagc gcgatcagct gctggaaaac
     7801 ctgcaggaag gcatggaagt taaaggtatc gttaagaacc tcactgacta cggtgcattc
     7861 gttgatctgg gcggcgttga cggcctgctg cacatcactg acatggcctg gaaacgcgtt
     7921 aagcatccga gcgaaatcgt caacgtgggc gacgaaatca ctgttaaagt gctgaagttc
     7981 gaccgcgaac gtacccgtgt atccctgggc ctgaaacagc tgggcgaaga tccgtgggta
     8041 gctatcgcta aacgttatcc ggaaggtacc aaactgactg gtcgcgtgac caacctgacc
     8101 gactacggct gcttcgttga aatcgaagaa ggcgttgaag gcctggtaca cgtttccgaa
     8161 atggattgga ccaacaaaaa catccacccg tccaaagttg ttaacgttgg cgatgtagtg
     8221 gaagttatgg ttctggatat cgacgaagaa cgtcgtcgta tctccctggg tctgaaacag
     8281 tgcaaagcta acccgtggca gcagttcgcg gaaacccaca acaagggcga ccgtgttgaa
     8341 ggtaaaatca agtctatcac tgacttcggt atcttcatcg gcctggacgg cggcatcgac
     8401 ggcctggttc acctgtctga catctcctgg aacgttgcag gcgaagaagc agttcgtgaa
     8461 tacaaaaaag gcgacgaaat cgctgcagtt gttctgcagg ttgacgcaga acgtgaacgt
     8521 atctccctgg gcgttaaaca gctcgcagaa gatccgttca acaactgggt tgctctgaac
     8581 aagaaaggcg ctatcgtaac cggtaaagta actgcagttg acgctaaagg cgcaaccgta
     8641 gaactggctg acggcgttga aggttacctg cgtgcttctg aagcatcccg tgaccgcgtt
     8701 gaagacgcta ccctggttct gagcgttggc gacgaagttg aagctaaatt caccggcgtt
     8761 gatcgtaaaa accgcgcaat cagcctgtct gttcgtgcga aagacgaagc tgacgagaaa
     8821 gatgcaatcg caactgttaa caaacaggaa gatgcaaact tctccaacaa cgcaatggct
     8881 gaagctttca aagcagctaa aggcgagtaa ttctctgact cttcgggatt tttattccga
     8941 agtttgttga gtttacttga cagattgcag gtttcgtcct gtaatcaagc actaagggcg
     9001 gctacggccg cccttaatca atgcagcaac agcagccgct taatttgcct ttaaggaacc
     9061 ggaggaatca tgaccaagtc agaattgata gaaagacttg ccacccagca atcgcacatt
     9121 cccgtcaaga cggttgaaga tgcagtaaaa gagatgctgg agcatatggc ctcgactctt
     9181 gcgcagggcg agcgtattga aatccgcggt ttcggcagtt tctctttgca ctaccgcgca
     9241 ccacgtaccg gacgtaatcc gaagactggc gataaagtag aactggaagg aaaatacgtt
     9301 cctcacttta aacctggtaa agaactgcgc gatcgcgcca atatttacgg ttaagttttt
     9361 tactcaaact tgaacgagag aaaagcacct gtcgggtgct tttttcattt ctctaatctg
     9421 gaactggaag ctgcctcgca gagttttgaa cagttttcac cctttcgtta aattcttctg
     9481 aatatgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag ttgcacactt
     9541 tccataaaca gggaggggtg catgaaaata acgacagtcg gtgtatgcat aattagcgga
     9601 atttttccgt tgctgatttt gccccaattg cctgggacat taacccttgc gtttctgact
     9661 ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat cgcgctgacg
     9721 ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgtgggc aggagaaacc
     9781 ttaactggcg cgacgcagga tgcaattgtt gagatcactg caactgacgg catgaccact
     9841 cattacggtc aaattactca tctacaaggt cgacgtatat tccctgcgtc aggtctcgtg
     9901 atgtatggcg aatatcttcc gcaagcggtt tgtgctggac aacaatggtc aatgaaactc
     9961 aaagttcgtg cagttcatgg ccaacttaat gatggcggct ttgatagcca gcgttatgcc
    10021 attgcccagc atcagccgct caccggccgt tttctgcagg caagtgtcat tgaaccgaat
    10081 tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc ctatccgtgg
    10141 aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccccaa agaaatcaaa
    10201 aatatcatgc gcgatactgg aacggcgcat ttaatggcga tatcgggatt gcacatcgct
    10261 tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct gcctgggcgc
    10321 tggatccact ggcaaatacc attaattggc ggaatctgct gtgctgcttt ttatgcctgg
    10381 ttgacgggaa tgcaacctcc tgcattgcgt accatggtgg cgcttgctac gtggggaatg
    10441 cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg tctggcggca
    10501 attttgctga tggatcctgt tgccattctc tcgcaaagtt tatggctctc tgccgctgcg
    10561 gtcgcggcat tgatattttg gtatcagtgg tttccctgtc ctgagtggca actgccgccg
    10621 gtattgcgtg cagttgtttc cctcatccat ctgcaactgg gaatcacact tctgcttatg
    10681 cccgtgcaaa tcgtcatatt tcatggcatt agtctgacct cgtttattgc aaatctatta
    10741 gcaattccct tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat ggttgtgcat
    10801 ttaagcgggc cgttaatcct ggagcaaggg ttatggtttc ttgccgaccg gtctttggct
    10861 ttacttttct gggggttaaa gagtttgccg gaagggtgga tcaacattgc tgaatgttgg
    10921 caatggctat cattttcccc atggttctta ctggtggtat ggcgattaaa cgcctggcga
    10981 acgttgccag caatgtgtgt ggctggaggc ttgctgatgt gctggccgct gtggcaaaaa
    11041 cctcgacctg acgagtggca gctgtacatg cttgatgtcg ggcaagggct ggcaatggtg
    11101 atagccagaa acggcaaagc gattctctat gacacaggac tggcctggcc tgaaggggat
    11161 agtgggcaac aactgattat cccctggctc cactggcata atcttgaacc ggaaggcgtt
    11221 attctgagcc atgaacatct ggatcaccgg ggagggctgg attcaatatt gcatatatgg
    11281 ccgatgttat ggatcagaag tccgttaaac tgggaacatc atcagccctg tgtgcgtggc
    11341 gaagcgtggc aatggcaagg attgcgtttc agcgcgcact ggcctttaca aggtagcaac
    11401 gataaaggaa ataaccattc ctgtgtggtt aaggttgatg acgggacgaa tagcattctt
    11461 ctaaccggtg atattgaagc cccagctgaa caaaagatgc taagccgtta ctggcagcaa
    11521 gtgcaggcaa cattgcttca ggtacctcac catggcagta atacctcatc atcgttgcca
    11581 ttaattcagc gagtgaatgg aaaagtggca ctcgcatcgg catcgcgcta taacgcatgg
    11641 cgactgccct ctaacaaagt taagcatcgc tatcaactgc aaggatatca atggattgat
    11701 actccacatc aaggtcaaac aacggtcaat ttttcagcgc aaggctggcg gattagcagc
    11761 ctcagggagc aaattttacc tcgttggtat catcagtggt ttggcgtgcc agtggataac
    11821 gggtagaata tgcggctatt tcaacaaatg ctggtttttt gaatgcataa cgacaaagat
    11881 ctctctacgt ggcagacatt ccgccgactg tggccaacca ttgcgccttt caaagcgggt
    11941 ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt catgttatcg
    12001 ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg
    12061 atgccgctgg tggtgatcgg gctgatgatt ttacgtggta tcaccagcta tgtctccagc
    12121 tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt
    12181 cacatgatgg gaatgccagt ttcattcttt gacaaacagt caacgggtac gctgttgtca
    12241 cgtattacct acgattccga acaggttgct tcttcttctt ccggcgcact gattactgtt
    12301 gtgcgtgaag gtgcgtcgat catcggcctg ttcatcatga tgttctatta cagttggcaa
    12361 ctgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg cgttgtatcg
    12421 aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt gaccaccagc
    12481 gcagaacaaa tgctgaaggg ccacaaagaa gtattgattt tcggtggtca ggaagtggaa
    12541 acgaaacgct ttgataaagt cagcaaccga atgcgtcttc aggggatgaa aatggtttca
    12601 gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt
    12661 ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac gattaccgtt
    12721 gttttctctt caatgattgc actgatgcgt ccgctgaaat cgctgaccaa cgttaacgcc
    12781 cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga cagtgagcag
    12841 gagaaagatg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga attccgcaat
    12901 gtcaccttta cttatccggg acgtgacgta cctgcattgc gtaacatcaa cctgaaaatt
    12961 ccggcaggga agacggttgc tctggttgga cgctctggtt cgggtaaatc aaccatcgcc
    13021 agcctgatca cgcgttttta cgatattgat gaaggcgaaa tcctgatgga tggtcacgat
    13081 ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc
    13141 catctgttta acgatacggt tgctaacaac attgcttacg cacggactga acagtacagc
    13201 cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat caataagatg
    13261 gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt
    13321 cagcgtattg ctatcgctcg agccttgttg cgtgatagcc cgattctgat tctggacgaa
    13381 gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact ggatgagttg
    13441 cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga aaaggcagac
    13501 gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt
    13561 gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg atcgaaaaaa
    13621 tctggtctgg tgaatcccct ttgtggcggc tattgctgcc actctcctgg ttgtatggcc
    13681 tggtgagtgg cgcgatccgt ctttgctata aactaaaact gaagcgcgcc tggcgtgccc
    13741 ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg caacggaaaa accccggtcg
    13801 ttgtctggct ggtggaacag ttgcaacagc gcggtattcg cgtgggggtc gtatcgcggg
    13861 gatatggtgg taaggctgaa tcttatccgc tgttattgtc ggcagatacc acaacagcac
    13921 aggcgggtga tgaacctgtg ttgatttatc aacgcactga tgcgcctgtt gcggtttctc
    13981 ccgttcgttc tgatgcggta aaagccattc tggcgcaaca ccctgatgtg cagatcatcg
    14041 taaccgacga cggtttacag cattaccgtc tggcgcgtga tgtggaaatt gtcgttattg
    14101 atggtgtgcg tcgctttggc aatggctggt ggttgccggc ggggccaatg cgtgagcgag
    14161 cggggcgctt aaagtcggtt gatgcggtaa tcgtcaacgg cggtgtccct cgcagcggtg
    14221 aaatccccat gcatctgctg ccgggtcagg cggtgaattt acgtaccggt acgcgttgtg
    14281 acgttgctca gcttgaacat gtagtggcga tggcggggat tgggcatccg ccgcgctttt
    14341 ttgccacgct gaagatgtgt ggcgtacaac cggaaaaatg tgtaccgctg gccgatcatc
    14401 agtctttgaa ccatgcggat gtcagtgcgt tggtaagcgc cgggcaaacg ctggtaatga
    14461 ctgaaaaaga tgcggtgaaa tgccgggcct ttgcagaaga aaattggtgg tatttgcctg
    14521 tagacgcaca gctttcaggt gatgaaccag cgaaactgct tacgcaacta accttgctgg
    14581 cttctggcaa ctagttacgc cgcggcagcg ttcgattgat ggagtcatga atgtcgctgc
    14641 cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc cgcacaaggc ctgttaaaca
    14701 aaccccgccg tcgagcgtcg ttggaggata ttccggcaac gatctcccgc atgtccttgc
    14761 tgcaaatcga taccatcaat attgttgccc gtagtccata tctggtgctt ttcagtcgtc
    14821 tgggaaatta tcctgcccag tggctggatg agtctctggc gcgtggcgaa ttaatggaat
    14881 actgggcgca tgaagcctgc tttatgccgc gtagcgactt tcgtcttatt cgccaccgca
    14941 tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc ctggatgcag gaacatgagg
    15001 cggaaattgc acagttaatt cagcatattc atgataaggg gccggtacgt tcagccgatt
    15061 ttgagcatcc tcgtaaaggt gcaagcggct ggtgggaatg gaagccgcat aaacggcatc
    15121 tggaaggttt atttactgcc ggaaaggtga tggtgattga acggcgcaac ttccagcgcg
    15181 tttatgattt aacccaccgt gtcatgcctg actgggatga tgagcgcgat ctcgtttcgc
    15241 aaacagaagc agaaatcatc atgctggata acagtgcgcg tagcctggga atattccgcg
    15301 aacagtggct ggcagattac tatcggctga aacgtccggc actggcggcg tggcgcgaag
    15361 cgagggctga acagcagcaa atcattgctg tgcatgttga aaaattgggc aatctttggc
    15421 tgcatgatga tttgctgccg ctactcgagc gagcgctggc cggaaagctc actgcaacgc
    15481 acagcgcggt actttcgcct tttgatcctg ttgtctggga tcgcaaacgc gcagagcagc
    15541 tttttgattt tagctaccgg ctggagtgct ataccccagc gccgaaacgc cagtatggct
    15601 attttgttct gccgttatta catcgtgggc aattagttgg gcgaatggat gccaaaatgc
    15661 atcgccagac aggcatcctt gaagttatct ctctgtggtt acaggaaggt attaaaccaa
    15721 cgacaacgct gcaaaaaggg ctacgtcagg cgattactga tttcgctaac tggcagcagg
    15781 caacgcgggt gacattagga tgctgcccgc aaggcctctt tactgattgc cgcaccggct
    15841 gggaaataga ccccgtcgca taaatgatta tgataagatt tatgcattca taacccgatc
    15901 catctggagg aactatggat catcgtctgc ttgaaatcat tgcctgcccg gtttgcaacg
    15961 gaaaactttg gtataaccag gaaaaacaag agcttatttg caaactggac aacctcgctt
    16021 tcccgctgcg tgatggcatt ccagtgttgc tggaaacgga agcccgcgtg ctgactgctg
    16081 atgagagtaa atcatgagtt ttgtggtcat tattcccgcg cgctacgcgt ccacgcgtct
    16141 gcccggtaaa ccattggttg atattaacgg caaacccatg attgttcatg ttcttgaacg
    16201 cgcgcgtgaa tcaggtgccg agcgcatcat cgtggcaacc gatcatgagg atgttgcccg
    16261 cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc gccgatcatc agtcaggaac
    16321 agaacgtctg gcggaagttg tcgaaaaatg cgcattcagc gacgacacgg tgatcgttaa
    16381 tgtgcagggt gatgaaccga tgatccctgc gacaatcatt cgtcaggttg ctgataacct
    16441 cgctcagcgt caggtgggta tggcgactct ggcggtgcca atccacaatg cggaagaagc
    16501 gtttaacccg aatgcggtga aagtggttct cgacgctgaa gggtatgcac tgtacttctc
    16561 tcgcgccacc attccttggg atcgtgatcg ttttgcagaa ggccttgaaa ccgttggcga
    16621 taacttcctg cgtcatcttg gtatttatgg ctaccgtgca ggctttatcc gtcgttacgt
    16681 caactggcag ccaagtccgt tagaacacat cgaaatgtta gagcagcttc gtgttctgtg
    16741 gtacggcgaa aaaatccatg ttgctgttgc tcaggaagtt cctggcacag gtgtggatac
    16801 ccctgaagat cttgagcgcg ttcgcgctga aatgcgctaa tttcacttca cgacacttca
    16861 gccaattttg ggaggagtgt cgtaccgtta cgattttcct caatttttct tttcaacaat
    16921 tgatctcatt caggtgacat cttttatatt ggcgctcatt atgaaagcag tagcttttat
    16981 gagggtaatc tgaatggaac agctgcgtgc cgaattaagc catttactgg gcgaaaaact
    17041 cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg ttgtgggctt tgtatgacag
    17101 ccagggaaac ccaatgccgt taatggcaag aagctttagt acgcccggaa aagcccgaca
    17161 actggcatgg aaaaccacca tgctggcaag aagtgggact gtccgtatgc cgactattta
    17221 tggtgtgatg acgcatgaag aacaccccgg ccccgatgtc ctgctactgg agcggatgcg
    17281 tggtgtttcg gtggaggcac cagcccgaac accagaacgc tgggaacaac tcaaagacca
    17341 aatcgttgaa gccttactgg cctggcaccg tcaggacagt cgcggttgcg tcggcgcggt
    17401 cgacaatact caggaaaatt tctggccctc atggtaccgg caacatgttg aagtgctatg
    17461 gaccacgctc aatcagttca ataacaccgg tctgacgatg caggataagc ggatcctgtt
    17521 tcgcactcgc gaatgtctcc cggcattatt tgaaggcttt aacgacaatt gtgtgctgat
    17581 tcacggtaac ttctgtttac gcagcatgtt gaaagattcg cgcagcgatc agttactggc
    17641 gatggtcggg ccgggactaa tgctttgggc accgcgagaa tacgaactgt tccgactaat
    17701 ggataattct ctggcggaag atttgctctg gagttacctg caacgcgcgc cagtggcgga
    17761 gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat gaagttgcgc aactggttaa
    17821 taccggacga tttagtcggc gcaacttcga tctggcatca aaatcactct tgccgtggct
    17881 cgcctgacga accttttagc cactgccaga tacggccaag cgtttcatag ccaacacgat
    17941 cgctatgcat caaccagacc ggagaaggga ttgcccgttc ccacgggtta agcggcgagt
    18001 cgatggccaa ctgattcgcc ggggctggta gcggatttaa cccttcctgc tgaaaaaaga
    18061 tcatcgcccg cggcagatgt gatgcggagg tcaccagcag gaaaggggcg tcaccaatcg
    18121 cctgtttcac tgctgcagct tcttcttcgg tatcttttgg caaatccagg gtgataattt
    18181 gctcgcgcgg cacgcccagc gattgcgcaa ctctggcacc tacttccgct gtactcaccg
    18241 tattggtttt tgctacgcct cccgtgaaga tcagttttga tcccggattt tcgcgccata
    18301 agcgaatacc ttcattcagg cgtggcagac tgttattgat taaattagag ctcggtgccc
    18361 actgcgggtt ccaggtataa ccacctccga gcaccacgat atagtccact ttttgcgaat
    18421 tattccatgt cggataggtg ctttcgatgg gacgcagcag gcggtcggct accggttgca
    18481 aactcaataa caagagcgcc agccacccaa tgctaataaa aatcttcccg gttttttgaa
    18541 aacggctaaa ccacagcagc gccaggccag cgccaataat caacaacatc agcggaagtg
    18601 gcagcaacat attgccaatc acttttttca gtgtaaaaag catcctttct ggttcctttt
    18661 ttaaccatat agcaagggat cgccagcgat attacaccag acgggttcat tctcgccgtg
    18721 gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc caaccggtgg agaaagcaat
    18781 gcaggatcgc aattttgatg atattgcgga aaagttttcc cgtaacattt acggcaccac
    18841 caaagggcag cttcgacagg ctattctgtg gcaggatctc gatcgcgtgc tggcggaaat
    18901 gggcccgcaa aaactgcgtg tgctggatgc tggcggtgga gaagggcaga ccgcaatcaa
    18961 aatggccgag cgtgggcatc aggtcatttt atgcgatctt tctgcgcaga tgatcgaccg
    19021 cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac atgcaattta tacattgcgc
    19081 cgctcaggat gttgcttcgc atttggaaac gcccgttgat ctgatattgt tccatgcggt
    19141 gctcgagtgg gtggctgatc cccgcagcgt attgcagacc ctgtggtcag tgttgcgtcc
    19201 aggcggcgtg ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc ataacatggt
    19261 cgccgggaat tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac ggacgctttc
    19321 gccagattat ccacgcgacc cggcgcaggt ttatctgtgg ctggaagaag ctggttggca
    19381 aattatgggt aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga aacaccagca
    19441 gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt tattgccgtc aggaaccgta
    19501 tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca aggataaagt
    19561 atgagtgaat tttcccagac agtccccgaa ctggttgcct gggccagaaa aaatgacttc
    19621 tccatctcgc tgccggtaga ccgactctct tttctgctgg cggttgccac gctgaacggc
    19681 gagcgtctgg atggtgagat gagtgaaggc gagctggtgg atgcattccg ccatgtgagt
    19741 gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg ccaataacgc gatcaacgac
    19801 atggtgcgtc aacgtctgct gaaccgcttt accagcgagc aggcggaagg gaacgcaatt
    19861 taccgtctga cgccgctcgg catcggcatt actgactact acatccgtca gcgcgagttt
    19921 tctacgctgc gtctttctat gcagttgtcg attgtggcgg gtgagctcaa acgcgcagca
    19981 gatgccgccg aagagggcgg tgatgaattt cactggcacc gtaatgtcta tgcgccactg
    20041 aaatattcgg tagcagaaat tttcgacagt atcgacctga cgcaacgtct gatggacgaa
    20101 cagcagcagc aggtgaagga cgatatcgcc cagttgctga acaaagactg gcgggcggcg
    20161 atttccagct gtgaattgtt gctttcggaa acttccggaa cgctgcgtga attgcaggat
    20221 acgctggaag cggcaggcga caaattgcag gctaatctgt tgcgcattca ggatgcgacg
    20281 atgacccatg acgatctgca tttcgtcgat cgtctggtgt tcgatctgca gagcaaactc
    20341 gatcgtatta tcagttgggg ccagcaatcc atcgacttgt ggattggcta cgaccgccac
    20401 gtacacaaat ttattcgtac cgcgatcgat atggataaaa accgcgtctt tgctcagcgg
    20461 ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaactta tgccaatgcc
    20521 gatcgtctgc tggatatgcg tgacgaagag atggcactgc gcgatgaaga agtgactggg
    20581 gaacttcctg aggatctgga atacgaagag tttaacgaga tccgcgaaca gctggcggcg
    20641 atcatcgaag aacaacttgc cgtgtacaaa accagacaag tgccgctgga tcttggtctg
    20701 gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc actttgacgt tgcgcgtatt
    20761 gttattgatc aggcggtacg tcttggcgta gcgcaagcag atttcaccgg actgccagcg
    20821 aaatggcagc cgattaatga ttacggagcc aaggtacagg cgcatgtcat cgacaaatat
    20881 tgaacaagtg atgccggtta agctggcgca ggcgctggcg aatccgttat ttccggcgct
    20941 ggacagcgcc ttacgttcag gacgccatat tggcctcgac gaactggata atcatgcatt
    21001 cctgatggat tttcaggaat atctggaaga gttttacgcg cgttataacg ttgagcttat
    21061 tcgcgcaccg gaagggttct tctatttacg cccacgttcc accactctta tcccgcgttc
    21121 cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc tgttatctct atctcagccc
    21181 ggaacggctg gcgaatgagg ggattttcac ccagcaggaa ctgtacgacg aactgctcac
    21241 cctggccgat gaagcaaaac tgctgaaact ggtgaacaac cgttcaaccg gttcagacgt
    21301 tgaccgtcag aagttgcagg agaaagtacg ttcttcgctc aaccgtctgc gtcgtttagg
    21361 catggtgtgg tttatgggcc acgacagcag caagttccgc attaccgaat cggtgttccg
    21421 cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc gcctgattcg
    21481 tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa ccgaagagag
    21541 ccagccagat agcggagagg aagaataatg attgaacgcg gtaaatttcg ctcactgacg
    21601 ctgattaact ggaacggctt ttttgcccga acttttgacc ttgacgagct ggtcacgacg
    21661 ctttccggcg gtaacggggc gggtaaatcc accacgatgg cggcgttcgt tacggcgctg
    21721 atccccgacc tgaccctgct gcatttccgt aacactacgg aagccggggc caccagcggt
    21781 tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg tctgttattc gatgctcgac
    21841 accattaact cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca ggttgccgga
    21901 cgcgatcgta aagtggatat caaaccattt gccattcagg gactgccgat gtcggtgcag
    21961 ccgacacagc tggtgaccga aaccctgaac gaacgccagg cgcgcgtgct gccgcttaac
    22021 gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt caactccatt
    22081 accgattacc actcgctgat gttcgatctg ggcatcatcg cgcgtcgtct gcgctccgca
    22141 tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg gatctccagt
    22201 gccattaccc gttctctgcg cgactacctg ttgccggaaa acagcggcgt gcgtaaagcg
    22261 ttccaggaca tggaagcggc gctgcgtgaa aaccgcatga cgctggaagc gattcgtgtc
    22321 acccagtcgg accgtgacct gtttaagcat ctgatcagcg aagctaccaa ctacgtggcg
    22381 gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc tcgacaaagc cctggagttt
    22441 cgtcgcgagt tacatacttc gcgtcagcaa ctggcggctg agcagtacaa acacgtcgat
    22501 atggcgcgtg agctggcaga gcacaacggt gccgaaggcg atctggaagc ggattatcag
    22561 gcggccagcg atcacctgaa cctggtgcaa accgcactgc gtcagcagga gaagatcgaa
    22621 cgctacgaag cggatctcga tgagctgcag atccgtctgg aagagcaaaa cgaagtggtg
    22681 gcagaagcca tcgaacgcca ggaagagaac gaggctcgcg cggaagctgc cgaactggaa
    22741 gtggacgagc tgaaaagcca gcttgctgac taccagcagg cgctggacgt ccagcaaacg
    22801 cgcgcgatcc agtataacca ggcgattgct gcacttaatc gtgccaaaga actgtgccat
    22861 ctgccggact taaccgccga cagcgccgcc gaatggctgg aaaccttcca ggcgaaagag
    22921 ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc gcaaaccgcg
    22981 cacagccagt ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc actggcgcgt
    23041 aacgaggcgt gggatgtcgc tcgcgaacta ttgcgcgaag gggtcgatca gcgtcacctg
    23101 gcagagcagg ttcagccgct gcggatgcga ttaagcgaac tggaacagcg tctacgcgag
    23161 cagcaagaag ctgagcgtct gctggcagat ttctgcaaac gtcagggcaa gaattttgat
    23221 atcgacgaac tggaagccct gcatcaggaa ctggaagcac gcattgcctc tctttccgat
    23281 agcgtgtcta acgcccgtga agagcgcatg gcactgcgcc aggagcagga acagctgcag
    23341 tctcgcattc agagtttgat gcagcgtgcg ccggtttggc tggcagcgca aaacagtctc
    23401 aaccagttga gcgaacagtg cggcgaagag tttacctcca gccaggacgt caccgaatat
    23461 ctgcaacagt tgctggagcg tgagcgagag gcgattgttg aacgcgatga agtgggcgcg
    23521 cgcaaaaacg ccgtcgatga agagatcgaa cgtttaagcc agcctggcgg ctctgaagat
    23581 cagcgtctga acgcgctggc ggagcgtttt ggtggtgtgc tgctgtcaga aatttatgac
    23641 gacgttagcc tggaagatgc gccgtacttc tcagcgctgt atggcccgtc acgccacgcc
    23701 atcgtggtgc cagatctgtc acaggtaact gaacacctgg aaggcttgac cgattgcccg
    23761 gaagatctct atctgatcga aggagatccg cagtcattcg atgacagcgt gttcagcgtt
    23821 gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg ttattcacgt
    23881 ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga aagcctccat
    23941 gccgagcgtg aagtgctttc cgaacgcttc gccacgctct cctttgatgt acagaaaact
    24001 cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc atctggcggt tgcgtttgag
    24061 tctgacccgg aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact ggagcgggcg
    24121 ttaagtaatc atgaaaatga taaccagcag cagcgtattc agtttgagca ggcgaaagag
    24181 ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc tgttggctga tgacagcctg
    24241 gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc cgcgcgtttt
    24301 gttcagcagt ttggcaatca actggcgaaa ctggaaccga ttgtttcggt attgcagagc
    24361 gacccggaac agttcgaaca gttaaaagaa gattacgcgt actctcagca gatgcagcgc
    24421 gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc gcactttagc
    24481 tattctgact cggcagaaat gcttagcggt aacagcgatc tcaacgaaaa actgcgtgaa
    24541 cgtctggaac aggcggaagc ggagcgtacc cgcgctcgcg aagcgttgcg cggtcacgca
    24601 gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga aaagttctta cgacaccaaa
    24661 aaagagctac tcaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg tgctgatagc
    24721 ggggcagaag agcgggcgcg tattcgccgt gacgagctgc atgcgcaact gagcaataac
    24781 cgttcacgcc gcaatcaact ggaaaaagcg cttaccttct gcgaagcgga gatggacaac
    24841 ctgacccgca aactgcgcaa gctggagcgg gattactttg agatgcgcga gcaggtagtg
    24901 accgccaaag cgggctggtg tgcggtgatg cgcatggtga aagataacgg cgttgagcgc
    24961 cgcttacacc gtcgtgagct ggcttatctc tccgctgatg atttgcgttc catgtcggat
    25021 aaggcgttag gtgcgctgcg tctggcggtg gcggataacg aacatctgcg cgacgtgctg
    25081 cgcatgtcgg aagatccgaa acgtccggag cgtaaaattc agttcttcgt ggcggtttat
    25141 cagcatctgc gtgaacgtat tcgtcaggat attattcgta ccgatgatcc ggtggaagct
    25201 atcgaacaga tggagattga acttagccgt ctgaccgaag aattaacctc ccgtgaacag
    25261 aaactggcga tcagttcccg cagcgtggcg aacatcattc gcaaaaccat tcagcgcgag
    25321 cagaaccgta tccgtatgct caaccagggg ttgcagaacg tatcgtttgg tcaggtgaac
    25381 agcgtgcgtc tcaacgtgaa cgtgcgtgaa acgcacgcca tgctactgga tgtgctctct
    25441 gaacagcacg agcagcatca ggatctgttt aacagcaacc gtttgacctt ctcggaagcg
    25501 ctggcgaaac tgtatcaacg tcttaacccg cagattgata tggggcagcg cacgccgcag
    25561 accatcggtg aagaactgct ggattaccgc aactatctgg aaatggaagt tgaggttaac
    25621 cgtggttccg atggctggct gcgcgcagag tctggtgcat tgtcgaccgg tgaggcgatt
    25681 ggtaccggta tgtcgattct ggtgatggtg gtacaaagct gggaagatga atctcgccgc
    25741 ctgcgcggta aagatatctc tccttgccgc ctgctgttcc tcgatgaagc agcgcgactg
    25801 gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgagc gtttgcaaat gcaactcatc
    25861 atcgcagcgc cggaaaatat cagcccggag aaaggcacca cctataaact ggtgcgtaaa
    25921 gtcttccaga ataccgaaca cgttcatgtc gtcggcctgc gaggatttgc gccgcaactc
    25981 cctgaaacgc ttccaggaac tgacgaagcg ccttctcagg cgagttaaaa ttaaggcggc
    26041 agcaatgccg ccttttcttt ttccgaaaac tccgtttctg cactaaaaaa gtggcacatt
    26101 acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta tcggcgttta
    26161 tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg tgagtattgg
    26221 cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat aaaaacaggg
    26281 ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc
    26341 tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg
    26401 gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg caagccacgg
    26461 caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa
    26521 tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt caactgttgt
    26581 atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa gccttccagc
    26641 aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa tgggtagagt
    26701 tactgaccga tcctggtgtt aacgggatgg cacgcgacgt ggtgctctct gatgcgatga
    26761 tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg ctatatagca
    26821 gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg cagctggcgc
    26881 tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat ccgcaatatg
    26941 cggcgatgca tgaatcgtta ctggccttac tctgtgacac caaaccgtgg ccccaactga
    27001 ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa
    27061 tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct ggcgatgaca
    27121 cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca gaaactaagc
    27181 cgatggataa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgc gccgcctacg
    27241 ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg
    27301 gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag cgtgctggcg
    27361 tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct accgggatca
    27421 tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag gtgctggatt
    27481 cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt gcccttaaca
    27541 acgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa gatattctgc
    27601 caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg atgcgcggct
    27661 ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc acggcctcga
    27721 atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg cgctataaat
    27781 tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac aatctgttca
    27841 agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct tccgatctgg
    27901 cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat gcgctgaagc
    27961 agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc tactacctga
    28021 cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac aattatgatc
    28081 tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc aggtaaatga
    28141 agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga ttctctgcag
    28201 cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc
    28261 tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg attatcatgg
    28321 acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg
    28381 ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc attttgacac
    28441 tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc agaggctata
    28501 ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa
    28561 agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc
    28621 gcaaaccggt gcaactcatt tccggttatc gttctattga taccaacaat gaactacgcg
    28681 cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg atggatttcc
    28741 atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg cgcgcaggtg
    28801 gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca gcacggcact
    28861 ggtagcaatc gcttaacgaa acaggggcag tatgaactat cgtattattc cggtcaccgc
    28921 attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg cactggtcga
    28981 tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc tgacactgat
    29041 gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg aactggcgca
    29101 acattacggc gtgccagttt ttggcccgga aaaagaagat gagttctggc tgcaaggctt
    29161 gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc cagatcgttg
    29221 gctgaacgaa ggcgatacca tcagcatagg gaatgtgact ttacaggtgt tacattgccc
    29281 tgggcatacg ccgggtcatg tagtgttttt tgatgatcgg gcaaagctgc tgatttctgg
    29341 cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg atcataatca
    29401 actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga catttattcc
    29461 gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct tcctgcaaga
    29521 cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg cctggatttc
    29581 tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg gcctacaaaa
    29641 tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag cgcatcaggc
    29701 aatgttacgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg tttttaatgc
    29761 ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg tcatcccggc
    29821 cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca gacgcagcac
    29881 ttgttctttt gtcaggccac tgaaggagaa catgccgttc tgtttgatga taaagctgaa
    29941 gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca tacgctgaat
    30001 acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag
    30061 gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc
    30121 cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca
    30181 agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg caacaatcag
    30241 ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca gaccacgggc
    30301 aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg agagttgtgc
    30361 cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc agcagccatg
    30421 gaacagcact acgtcgccag cctgagcttc attcaggctg ttaatcagtg catcgaagtc
    30481 aagagtgtga ttttccgcat cataataagc gtattcacga acttccagac ctgcagagtt
    30541 aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct taacgctggt
    30601 attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg gagtctgtgc
    30661 cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca gttcctgagt
    30721 gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg tttcattttc
    30781 gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc ccgtctcatc
    30841 tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac gaaacagatc
    30901 ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt
    30961 ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta caaaattcca
    31021 actattactg atgaaaactc aggctgtttt tgcaagacgt gagattgctc tggaaggtat
    31081 aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg tgctattaga
    31141 actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt ttgttgtcag
    31201 aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg aagtagtagg
    31261 ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct acgtctttcg
    31321 ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg tattgcgcaa
    31381 ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat ttattagtga
    31441 tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg ttcgcgtcgt
    31501 acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt tgagcttctt
    31561 gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct tcgtattcgt
    31621 agctgataga accgccaaca ccgtcgccgt tagagcggcg tgcagtgtca cgctcgtttt
    31681 tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag ttggagttac
    31741 gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat gccgtgtcac
    31801 cgccaaactc aggcagcata tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac
    31861 ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt
    31921 tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta tattcccact
    31981 gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca agacgggcat
    32041 aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggag aaataatgca
    32101 gaccgacagc tttaccgtac agatctactt tgttgccatc tttgttatag atttctgcag
    32161 cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata ttgcgcttca
    32221 tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt caataagttc
    32281 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
    32341 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttgg
    32401 tttcaaaaag aaaaaatatg ta
//
LOCUS       DAZFZC010000052        32254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFZC010000052 DAZFZC010000000
VERSION     DAZFZC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.052"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..356)
                     /locus_tag="ACHKXP_002768"
     CDS             complement(<1..356)
                     /locus_tag="ACHKXP_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFO7647742.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSA"
     gene            complement(387..1079)
                     /gene="fimC"
                     /locus_tag="ACHKXP_002769"
     CDS             complement(387..1079)
                     /gene="fimC"
                     /locus_tag="ACHKXP_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO7647743.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1299..1841)
                     /gene="fimA"
                     /locus_tag="ACHKXP_002770"
     CDS             complement(1299..1841)
                     /gene="fimA"
                     /locus_tag="ACHKXP_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7647744.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2312..3178
                     /gene="folD"
                     /locus_tag="ACHKXP_002771"
     CDS             2312..3178
                     /gene="folD"
                     /locus_tag="ACHKXP_002771"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO7647745.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            3180..3392
                     /gene="ybcJ"
                     /locus_tag="ACHKXP_002772"
     CDS             3180..3392
                     /gene="ybcJ"
                     /locus_tag="ACHKXP_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO7647746.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3500..4021
                     /gene="ybcI"
                     /locus_tag="ACHKXP_002773"
     CDS             3500..4021
                     /gene="ybcI"
                     /locus_tag="ACHKXP_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7647747.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4057..5442)
                     /gene="cysS"
                     /locus_tag="ACHKXP_002774"
     CDS             complement(4057..5442)
                     /gene="cysS"
                     /locus_tag="ACHKXP_002774"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO7647748.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5616..6110
                     /gene="ppiB"
                     /locus_tag="ACHKXP_002775"
     CDS             5616..6110
                     /gene="ppiB"
                     /locus_tag="ACHKXP_002775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO7647749.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            6113..6835
                     /gene="lpxH"
                     /locus_tag="ACHKXP_002776"
     CDS             6113..6835
                     /gene="lpxH"
                     /locus_tag="ACHKXP_002776"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO7647750.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            6953..7462
                     /gene="purE"
                     /locus_tag="ACHKXP_002777"
     CDS             6953..7462
                     /gene="purE"
                     /locus_tag="ACHKXP_002777"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO7647751.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            7459..8526
                     /gene="purK"
                     /locus_tag="ACHKXP_002778"
     CDS             7459..8526
                     /gene="purK"
                     /locus_tag="ACHKXP_002778"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO7647752.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(8721..9614)
                     /gene="ybcF"
                     /locus_tag="ACHKXP_002779"
     CDS             complement(8721..9614)
                     /gene="ybcF"
                     /locus_tag="ACHKXP_002779"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7647753.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSGGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9611..10426)
                     /gene="ylbF"
                     /locus_tag="ACHKXP_002780"
     CDS             complement(9611..10426)
                     /gene="ylbF"
                     /locus_tag="ACHKXP_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7647754.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWMLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(10437..11696)
                     /gene="ylbE"
                     /locus_tag="ACHKXP_002781"
     CDS             complement(10437..11696)
                     /gene="ylbE"
                     /locus_tag="ACHKXP_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7647755.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(11706..13373)
                     /gene="fdrA"
                     /locus_tag="ACHKXP_002782"
     CDS             complement(11706..13373)
                     /gene="fdrA"
                     /locus_tag="ACHKXP_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7647756.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATSNDICVTIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     SRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLITDLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            13690..14739
                     /gene="allD"
                     /locus_tag="ACHKXP_002783"
     CDS             13690..14739
                     /gene="allD"
                     /locus_tag="ACHKXP_002783"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO7647757.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEVLTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            14761..15996
                     /gene="allC"
                     /locus_tag="ACHKXP_002784"
     CDS             14761..15996
                     /gene="allC"
                     /locus_tag="ACHKXP_002784"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO7647758.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTLEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAILRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLAIMLYQLAWQK"
     gene            16007..16792
                     /gene="allE"
                     /locus_tag="ACHKXP_002785"
     CDS             16007..16792
                     /gene="allE"
                     /locus_tag="ACHKXP_002785"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO7647759.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNS
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(17020..18165)
                     /gene="glxK"
                     /locus_tag="ACHKXP_002786"
     CDS             complement(17020..18165)
                     /gene="glxK"
                     /locus_tag="ACHKXP_002786"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO7647760.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(18187..19488)
                     /gene="ybbY"
                     /locus_tag="ACHKXP_002787"
     CDS             complement(18187..19488)
                     /gene="ybbY"
                     /locus_tag="ACHKXP_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO7647761.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(19545..20906)
                     /gene="allB"
                     /locus_tag="ACHKXP_002788"
     CDS             complement(19545..20906)
                     /gene="allB"
                     /locus_tag="ACHKXP_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO7647762.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDVHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHGYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(20966..22420)
                     /gene="ybbW"
                     /locus_tag="ACHKXP_002789"
     CDS             complement(20966..22420)
                     /gene="ybbW"
                     /locus_tag="ACHKXP_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO7647763.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIATIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(22589..23467)
                     /gene="glxR"
                     /locus_tag="ACHKXP_002790"
     CDS             complement(22589..23467)
                     /gene="glxR"
                     /locus_tag="ACHKXP_002790"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7647764.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(23567..24343)
                     /gene="hyi"
                     /locus_tag="ACHKXP_002791"
     CDS             complement(23567..24343)
                     /gene="hyi"
                     /locus_tag="ACHKXP_002791"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO7647765.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKHVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(24356..26137)
                     /gene="gcl"
                     /locus_tag="ACHKXP_002792"
     CDS             complement(24356..26137)
                     /gene="gcl"
                     /locus_tag="ACHKXP_002792"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO7647766.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(26227..27042)
                     /gene="allR"
                     /locus_tag="ACHKXP_002793"
     CDS             complement(26227..27042)
                     /gene="allR"
                     /locus_tag="ACHKXP_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO7647767.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(27120..27602)
                     /gene="allA"
                     /locus_tag="ACHKXP_002794"
     CDS             complement(27120..27602)
                     /gene="allA"
                     /locus_tag="ACHKXP_002794"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO7647768.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            27832..28758
                     /gene="allS"
                     /locus_tag="ACHKXP_002795"
     CDS             27832..28758
                     /gene="allS"
                     /locus_tag="ACHKXP_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO7647769.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            28827..29921
                     /gene="mnmH"
                     /locus_tag="ACHKXP_002796"
     CDS             28827..29921
                     /gene="mnmH"
                     /locus_tag="ACHKXP_002796"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO7647770.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEVGINYPLVEGGYKVLRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            30037..30402
                     /gene="ylbG"
                     /locus_tag="ACHKXP_002797"
                     /pseudo
     CDS             30037..30402
                     /gene="ylbG"
                     /locus_tag="ACHKXP_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(30923..31096)
                     /locus_tag="ACHKXP_002798"
     CDS             complement(30923..31096)
                     /locus_tag="ACHKXP_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647771.1"
                     /translation="MAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFI
                     TNIGAGLPDACAE"
     gene            complement(31153..31413)
                     /locus_tag="ACHKXP_002799"
     CDS             complement(31153..31413)
                     /locus_tag="ACHKXP_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647772.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFLIFP"
     gene            complement(31394..32101)
                     /locus_tag="ACHKXP_002800"
                     /pseudo
     CDS             complement(31394..32101)
                     /locus_tag="ACHKXP_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(32104..>32254)
                     /locus_tag="ACHKXP_002801"
     CDS             complement(32104..>32254)
                     /locus_tag="ACHKXP_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO7647773.1"
                     /translation="EEEKPYLITELPDSWVVEGAKLPYEVAGGVFIIEINKKNGCVLN
                     FLHSK"
BASE COUNT         8128 a         8566 c         7777 g         7783 t
ORIGIN      
        1 aatgcactgc tattaaggcc aattcgttca agaagtacct ggttaaaaca gggcattaac
       61 ccacctgatt tatcacctgt attttctgtt gtcgattcaa atacgatatc ctgcgaacca
      121 atgaactcat cattacgcca gagatccacc cgatacacgc ctgcaggttg atgattacct
      181 ctctcaaagc gcgataaatc agcaacggat gtgccattct ctaataaaaa tgccgggttg
      241 aaataacttt ccgcatagct taaaaaggag ggtaaaatgg caagagatga ataacaaatt
      301 ccggccagac accaggtata cctctgcgga atatccgtag tagtgggtat tttcataatt
      361 agctctgata ttgtcagatg attcggttag tttaagttca cttctctgac cggagttact
      421 gaaccataat cattaacggt ctgaaatttc agctttccct gcactcctga gggtaaggga
      481 atttgtgaat taactctggg tgcagccata gcattcccca acttttggct gcccacttgt
      541 aagttaacca gggtgacata aaaaggtgat ggattatgaa cattgatata gttaccggat
      601 cgcgaaaact tgagtgtgtc cggcgcttct gcgggtaatt cttgtaattg aattggacgg
      661 agaaataatt tcatgcgcga taaaatcgcc agttgcaaaa cattcctgcc gttcaatgca
      721 tttttatcta ccgaagggat cgtcttaaca ttcatccaga acagagactc acgatctgct
      781 gccagcggtg gaccggtgta aataatacgc aaagtatttt cgcttttggg ttcactaaca
      841 aacagtggcg gtgtaatgat gaatgacttt tcttttacac cgctgctgtt ttcaatccac
      901 gaattgacca gaaagcgctc attggtatgg ctatttctaa tccataccgc agtctgttta
      961 gcatcagcgg gataaataat acgcgtggca cctaatgcga tccctccggc agcatgggcg
     1021 ctggccgaaa tgattaaata aaatataatg agcatcaata actttatttt agtcatcata
     1081 gttaatacgc cttgtgtatt cctgacggca aaaataagga atataaagaa atgattgctt
     1141 tggtttctta caacacatca ttcccttttc atttaataaa gcggcatgat acaaagtgaa
     1201 catgatttcg atttacattg ccaccatacc gcttcagaca accggtacat tactttgtta
     1261 taagacgtga tatataattc aaaacaacgt ggttttgatt attcatatct cataataaaa
     1321 gtcgcgtcag catttgcttg ccctgctgat gcacttgtac cggtgccttt ataacgtgca
     1381 gaaaaatgaa gaacgttggt cccggggatc aagttctggt tggttgagaa gctattccca
     1441 tcaggtttga gaattgcgga tgtattatca agtatttcaa taccgacacc cgttgctgtt
     1501 gttgtattgg tactggatgc aatggccaat aaattatcat tgattgcatc agcctgacca
     1561 gaaaatgcaa cggcagcatt agcggcaaca acaggatcgc agtcgttcaa ctgaatggtg
     1621 aatggaatta atgcagaggt attaccaaca gcattgaaaa tatcggtacg atattgaccg
     1681 agtgtgacaa cctggtctgc cgagtcagta ttcactgaac aggcagcatt caccagttcg
     1741 ccttcaaagt gaattgtacc gccatctaca acggcagcat aactacccgt agcggcgaat
     1801 agtgcggcag ccagcgcaga cgaaataaat cttaatttca tatatattcc ttcaatctca
     1861 tttatcgact ccacatccgt atataaccga ttactttatt taagacactg atagtagtaa
     1921 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     1981 gagagccccg ttagatgaat acttccgcgc ataatatatt caacacaaat atagacctga
     2041 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2101 taatccttag acaaggaaat aaatcagttc cagatttaca acgccatcat ggacgaaaaa
     2161 tgaagctttc agtctcagcg acggtgcgcc tcaccttcgc aagaggtcgc ttcacgcgat
     2221 aaatctgaaa cgaaacctga cagcgcgccc cgcttctgac aaaataggcg catccccttc
     2281 gacctacgta acagatggaa tcctctctct gatggcagca aagattattg acggtaaaac
     2341 gattgcgcag caggtgcgct ctgaagttgc tcaaaaagtt caggcgcgta ttgcagccgg
     2401 actgcgggca ccaggactgg ccgttgtgct ggtgggtagt aaccctgcat cgcaaattta
     2461 tgtcgcaagc aaacgcaagg cttgtgaaga agtcgggttc gtctcccgct cttatgacct
     2521 cccggaaacc accagcgaag cggagctgct ggagcttatc gatacgctga atgccgacaa
     2581 caccatcgat ggcattctgg ttcaactgcc gttaccggcg ggtattgata acgtcaaagt
     2641 gctggaacgt attcatccgg acaaagacgt ggacggtttc catccttaca acgtcggtcg
     2701 tctgtgccag cgcgcgccgc gtctgcgtcc ctgcaccccg cgcggtatcg tcacgctgct
     2761 tgagcgttac aacattgata ccttcggcct caacgccgtg gtgattggcg catcgaatat
     2821 cgttggccgc ccgatgagca tggaactgct gctggcaggt tgcaccacta cagtgactca
     2881 ccgcttcact aaaaatctgc gtcatcacgt agaaaatgcc gatctattga tcgttgccgt
     2941 tggcaagcca ggctttattc ccggtgactg gatcaaagaa ggcgcaattg tgattgatgt
     3001 cggcatcaac cgtctggaaa atggcaaagt tgtgggcgac gtcgtgtttg aagacgcggc
     3061 taaacgcgcc tcatacatta cgcctgttcc cggcggcgtt ggcccgatga cggttgccac
     3121 gctgattgaa aacacgctac aggcgtgcgt tgaatatcat gatccacagg atgagtaaca
     3181 tggcgacatt ttctttaggt aaacatccgc acgttgagct gtgcgacttg ctgaaactgg
     3241 aaggctggag cgaaagcggc gcgcaggcga aaatcgcgat tgccgaaggc caggtgaaag
     3301 tcgacggtgc ggttgaaacg cgcaaacgct gcaaaatcgt cgccggtcag acagtgagtt
     3361 ttgcaggtca cagcgtacag gttgttgcct gatccctttc ccggcctgta tcaacaggcc
     3421 ggttctcttt tcagactttt gccctctacg ccccgttcat ccatgctgat agcatcgaca
     3481 ttttgccgaa gcgaccatca tgccaaccgt tattacgcac gccgccgttc ccctatgcat
     3541 tggtttaggg ttgggttcga aagtcatccc tccgcgcctg ttatttgccg gaatcatcct
     3601 ggcgatgctg cccgatgccg acgtcttgtc gtttaatttt ggcgttgctt acggcaatgt
     3661 ttttggtcat cgcgggttta cccattcgct ggtgtttgcg tttgttgtcc cgctgttatg
     3721 tgtgctgatt ggacgtcgat ggttcagggc tgggctgatt cgctgctggc tgtttttaac
     3781 cgtctcgttg ctatcgcaca gcttgctgga ttcggtaacc actggcggta aaggggttgg
     3841 ctggctgtgg ccgtggtcag atgaacgctt tttcgctccc tggcaggtga ttaaagtcgc
     3901 gccgtttgca ttgtctcgtt acaccacagc gtacgggcat caggtgatta tttctgaatt
     3961 gatgtgggtg tggttgccgg ggatgctact gatgggaatg ttgtggtggc gcagacgata
     4021 accggatgcg aaaactcgca tccggcaata gcgcaattac ttacgacgcc aggtggtccc
     4081 ttgcgggcca tcttccagca cgatccccat ctcgttaaga cgatcacgcg ccgcatccgc
     4141 cgccgcccag tctttcgctt tacgggcatc cagacgctgt tgaattaacg cttcaatctc
     4201 agccacttcg ctgtcgtctg cctgcgcgcc gctttgcagg aacgcttccg gttcttgctc
     4261 cagcaggccc aatacagcgg aaagtttacg caggtgagat gccattgcat tcgccgctgc
     4321 catatcttct gctttcagac ggtttacttc acgcgccata tcaaacagta cggaataggc
     4381 ttccggggtg ttgaaatcgt cgtccatcgc ttcaataaag cgcgcttcaa acgcttcgcc
     4441 accggcaggc gcaacggttt tatctgtgcc gcgcagcgca gtgtagagac gctccagcgc
     4501 cgcacgcgcc tgcttcaggt tctcttcgct gtagttcaac tggctgcgat agtggcccga
     4561 catcaggaag taacgcacgg tttccgcgtc gtagtatttc agcacatcgc gcacggtaaa
     4621 gaagttaccc agcgatttgg acatcttctc gcggtcaacc atcaccatcc ccgagtgcat
     4681 ccagtagttc acatactgac catcatgggc acaggtggac tgcgcgattt cgttttcgtg
     4741 gtgcgggaac atcaggtctg aaccgccgcc gtggatatca aagtggttac ccagctgctt
     4801 gcagttcatt gccgaacatt caatgtgcca gccaggacga cccgcgcccc acggagacgg
     4861 ccagctcggt tcgccctctt tcgacatctt ccacagaacg aagtccattg ggttgcgttt
     4921 gtcgtcgacc acgtcaacgc gcgcgcctgc ctgcagctgg tcgagatcct gacgcgacag
     4981 cacgccataa gttggatcgg tcgggacgtc gaacatcacg tcgccgttgt ccgccacata
     5041 agcgtgacct ttggcgatca gttgttcagt gagttcaata atttctgcga tatggtgcgt
     5101 cgcgcgcggc tccatatccg ggcgcagaat gttcaacgca tcaaaatctt tgtgcatttc
     5161 ggcgatcatg cgatccacca gcgccacaaa gctttcgcca ttttcattgg cgcgtttgat
     5221 gattttgtcg tcgatatcgg taatgttgcg cacatacttc agcttatagc cgaggaaacg
     5281 cagatagcgc gcaaccacgt caaaagcaac aaaggtacgc ccgtgaccga tatgacagag
     5341 atcgtaaacg gtgattccac acacgtacat gccgacttcc ccggcgtgaa taggcttaaa
     5401 ttcctctttt tggcgtgtca gagtattgaa gatttttagc atcgaagatt ccgtttagac
     5461 atgtgtgggt aattgagttg cgtataatac ccatatttcc cgcgcgaatc agcatacatt
     5521 gcgtgatgat ccgatcgtgc ggttatgcta taacaccacc ctatatatga cccgaactgg
     5581 gttgaagcac caatcaaacg gaacaggatg caaaaatggt tactttccac accaatcacg
     5641 gcgatattgt catcaaaact tttgacgata aagcacctga aacagttaaa aacttcctgg
     5701 actactgccg cgaaggtttt tacaacaaca ccattttcca ccgtgttatc aacggcttta
     5761 tgattcaggg cggcggtttt gaaccgggca tgaaacaaaa agccaccaaa gaaccgatca
     5821 aaaacgaagc caacaacggc ctgaaaaata cccgtggtac gctggcaatg gcacgtactc
     5881 aggctccgca ctctgcaact gcacagttct tcatcaacgt ggttgataac gacttcctga
     5941 acttctctgg cgaaagcctg caaggttggg gctactgcgt gtttgctgaa gtggttgacg
     6001 gcatggacgt ggtagacaaa atcaaaggtg ttgcaaccgg tcgtagcggt atgcaccagg
     6061 acgtgccaaa agaagacgtt atcattgaaa gcgtgaccgt tagcgagtaa tcgtggcgac
     6121 actctttatt gcagatcttc atctctgcgt ggaagaaccg gcgatcaccg ccggttttct
     6181 gcgtttttta gcgggggaag cccgcaaggc cgacgcgctg tatattcttg gcgatctgtt
     6241 tgaagcatgg attggcgacg acgatcccaa cccactccat cgccagatgg cggcagcgat
     6301 caaagcggtg tccgattccg gcgttccctg ttatttcatt catggcaacc gtgattttct
     6361 gctcggcaaa cgctttgccc gtgaaagcgg catgacatta ttgccggaag aaaaggtgct
     6421 cgaactttat ggtcgccggg tgttgattat gcatggcgac acgctgtgca ccgatgacgc
     6481 gggttatcag gcttttcgcg ccaaagtcca caaaccctgg ctgcaaacgc tattcctcgc
     6541 cctgccgttg tttgtgcgca aacgcattgc cgcgcgaatg cgagcgaaca gtaaagaagc
     6601 caacagcagt aaatcgctgg cgatcatgga cgttaaccaa aacgcggtag tcagtgcgat
     6661 ggaaaaacat caggtgcaat ggctgatcca cgggcatacc catcgcccgg cggtacatga
     6721 acttatcgcc aaccagcaac ctgcttttcg cgtggtactc ggtgcctggc atacagaagg
     6781 ttcgatggtg aaagtcacgg cggatgacgt tgagctgatt cattttccgt tttaaaaaac
     6841 ccgcaacttt gctgatttca cagccacgca accgttttcc ttgctctctt tccgtgctat
     6901 tctctgtgcc ctctaaagcc gagagttgtg caccacagga gttttaagac gcatgtcttc
     6961 ccgcaataat ccggcgcgtg tcgccatcgt gatggggtcc aaaagcgact gggctaccat
     7021 gcagttcgcc gccgaaatct tcgaaatcct gaatgtcccg caccacgttg aagtggtttc
     7081 tgctcaccgc acccccgata aactgttcag cttcgccgaa agcgccgaag agaacggtta
     7141 tcaggtgatt attgcgggcg caggcggcgc agcgcatctg ccaggcatga ttgccgccaa
     7201 aacgctggtg ccggtgctgg gcgtgccagt acagagcgcc gcactgagcg gtgtcgatag
     7261 cctctactcc atcgtacaaa tgccgcgcgg cattccggtg ggtacgctgg cgattggtaa
     7321 agctggcgcg gcaaacgcgg cgttactggc agcacaaatt cttgcgactc atgataaaga
     7381 actgcaccag cgtctgaatg actggcgcaa agcccagacc gacgaagtgc tggaaaaccc
     7441 ggacccgcga ggtgcggcat gaaacaggtt tgcgtcctcg gtaacgggca gttaggccgt
     7501 atgctgcgtc aggcaggcga accgttaggc attgctgtct ggccagtcgg gctggacgct
     7561 gaaccggcgg cggtgccttt tcaacaaagc gtgattaccg ctgagatcga acgctggccg
     7621 gaaaccgcat taacccgcga gctggcgcgc catccggcct ttgtgaaccg cgatgtgttc
     7681 ccgattattg ctgaccgtct gactcagaag cagcttttcg ataagctcca cctgccgact
     7741 gcaccgtggc agttacttgc cgaacgcagc gagtggcctg cggtgtttga tcgtttaggt
     7801 gagctggcga ttgttaagcg tcgcactggt ggctatgacg gtcgcggtca atggcgttta
     7861 cgcgccgatg aaaccgagca gttaccggct gaatgctacg gcgaatgtat tgtcgagcag
     7921 ggcataaact tctctggtga agtgtcgctg gttggcgcgc gcggctttga tggcagtacc
     7981 gtgttttatc cgctgacgca taacctgcat caggacggta ttttgcgcac cagcgtcgct
     8041 ttcccgcagg ccaatgcgca gcagcaagcg caagccgaag agatgctttc ggcgattatg
     8101 caggagctgg gctatgtggg cgtgatggcg atggagtgtt ttgtcacccc gcaaggtctg
     8161 ttgatcaacg aactggcacc gcgtgtgcat aacagcggtc actggacaca aaacggtgcc
     8221 agcatcagcc agtttgagct gcatctgcgg gcgattaccg atctgccgtt accgcaacca
     8281 gtggtgaata atccgtcggt gatgatcaat ctgattggta gcgatgcgaa ttatgactgg
     8341 ctgaaattgc cgctggtgca tctgcactgg tacgacaaag aagtccgtcc ggggcgtaaa
     8401 gtggggcatc tgaatttgac cgacagcgac acatcgcgtc tgactgcgac gctggaagcc
     8461 ttaatcccgc tgctgccgcc ggaatatgcc agcggcgtga tttgggcgca gagtaagttc
     8521 ggttaactgg tgctctattc ttgccggatg cggcgtaaac gccttatccg gcctaccgat
     8581 ccggtaccca ttgtaggcct gataagatgc gtcaagcatc gcatcaggca ttgtgcacca
     8641 attgccggat gcggcaccgg ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     8701 caaatgtcta atgcctacga ctacagcgaa atacaggtcc ccgcttcgcc cgccagcgtc
     8761 tcttcaattc gcgataacgc cccaatccac gcgggtttac caccgcttct gacataacca
     8821 ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg cgctaactca
     8881 tccggtgtgg catggcgaat ggcgcgttgc tgcggcgttc cccagttttc atataccgca
     8941 tcagcatcag tgaggatcac cagcccatcg gcgttaatct gctcagcgag caacgcagca
     9001 gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac aggcacaccg
     9061 ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat ggcttcgctg
     9121 tcgagaattt tacgcggttg cggagacgcc accacccggc gcaaatattt accatcacgt
     9181 ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg ataaaccgga
     9241 ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc aatgcgcgtc
     9301 agcaccgtcg tcacgggcgg catctgcggc tgtgcgctca aactctgcgc cagcatatag
     9361 ccaatcatcc cctgactttc tgccaccagc acatccagcg gatacggttc tacctctttc
     9421 cacgccagat tctgcaatgc cagcagcccc acctgcggcc cgttgccgtg aacaatcgcc
     9481 agccgataag aacgggccag gcgcgccagc gcgggtacag cactggcgat attgcgatat
     9541 tgattttctg ccgtcagcgc ctcaccgcgc tggagtaagg cgtttccccc aagagctaca
     9601 accagtgttt tcatggtttt ccttgtaata attgttgacc aagccagaac cccagcagtg
     9661 tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca gcggtacgtt
     9721 tcgggcaact cagagcatga acaaagtgca ggagtggcga ggcgaaatat ccttgcgcgg
     9781 cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgcagattg tcggaacagg
     9841 caaaaaactg gcgaccggag cgcgcatcta aagcgccata ataccaggcc gccagcagca
     9901 ttccgctcag cgtgtcatca tgactcggcg ttaatccggg gcctttaccc agccagtgcc
     9961 gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac tggcgtaact
    10021 cagcaggcag cggatcgctt gccgccaacg ccagtggccc gaaaagcccg gtttcctccg
    10081 cgcgttgcat ccatgcagct gcaagtggtt gaggatgcgc aggcggcgta atacgcagca
    10141 aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct tgagccacaa
    10201 cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc gcacggcgaa
    10261 gcatccatcc tccggggccg aaaccactac cctgacgatg cagcgttaac agttcgccgc
    10321 tttccgtcat caggttaatc gcccgtcgcc acactcccgc taaccgccag gactggcgat
    10381 aattcggtgc actacggctg gcaagcagag gatggatgat cgtcattgcg cgttcctcaa
    10441 ccaatcccca tgctttcagc cagtgcttcc agcgcctgtt caaaacacgc cagcggtgcc
    10501 cgcacagtgc ctgcgccaat ctgcccgatc cccgcctctt tatgggcgat accggtattg
    10561 atgagtggcg taatgccggt ttctaccacg cgacgaatgt ccagtcccag gcacgcgccc
    10621 tgaaaatccc agcctgggat ctgcaactgc atattgcgct caaggtaaat ttccgccatc
    10681 tcttcagata ccgctctggc cgcttccatg ccacccgcac cgacaaagcg cgttacgcca
    10741 ggcgctgcga tcattgccgc acctccgata ccaaaggttt cggtaatcgc gctatcgccc
    10801 atatccgggt tcgcctgctc ctgcgagaag ccggtgaaaa acagaccttg cggagtgttt
    10861 acaggcgcag taaaccagcg ttcgcccagc ccgcttaccc gaatcccgaa catattgccg
    10921 ttgcgggtca ttgccgtgac gatgctgcct gcgcggatca tcgcgccagc atccatcgcc
    10981 gccttgcagt aagccatcgc gaggttgagg aagaactgat cggtcacgct gaggaaatcc
    11041 atcacttcgg cgatgtgctg tttatcatga tcgaggcgag caatttgtgg ggccagcgca
    11101 cgcattaaca gtgcagagga agcaatattg cgttgatgga actcatcgcc catcgtaatg
    11161 ccctgcgcca tcatcgccgt gagatcgata ccgcgctcca tgcgccccag cgccgcgctt
    11221 aatactggca ttaacacatc gcgcatccag cgatggcgag tcaggacatc ttcgccgtaa
    11281 gcgccaaaac gcatcacttt gccgatacct tcgttgaggt tgcagtacgc ccggttgccg
    11341 tcggtcacgt tctcaaccac cagcatcggc atactggcag aagtaatacc gcccattgga
    11401 ccgacggcat tcacatggtg acaaggaatg aagttcactt ccccctgctc cagtattgcc
    11461 agcgcctgcg cttcatcttt cgcccagcct tcgaacagac atgcgcccac gcacgcccct
    11521 ttcatgggtc cggtcatctc ctgccagcgc attggcggcc cggcgtgaag cagtgttttg
    11581 ccctcgttta gttcgctgat aagtgaagaa gccggttgca catccagcca gtgtggacga
    11641 gcgcgacgaa tttgttcgat aaccgcagca ttggcttgcg ccactgatgt aaacatggga
    11701 accccttatt gcaaacgttc taataaacga gccagttttt tattgccgcc ggcgactggc
    11761 gaccattggt aatgcacaac cggtttgctg gcgctttgta gctccagcgc aaagctgcgt
    11821 aatccgatgt taatcacggc gacgttttcc agtaatgacg gtgtgtgttg ctgagtggca
    11881 ggcgaaagcg gacgaatcag tgccgccgcc agcaaggtgg cttccggcag cgaactcact
    11941 accgcgatcc ctgcatcttc cagcgtggcg atttgctgcg agcggcattg cgggtcacgt
    12001 tcagtgcctg tcaccgtggc aatggcatac agtggttgat tgtctgaacg cgcagcacag
    12061 gctttttgcc aggcgcttac cagcgaggcg gcaggatcgg cggtcgcacc gaagccaatc
    12121 acgacatcaa gcagcagcac acgcacttgc ggtttagcgc cgagatcggt aattaactgg
    12181 ttgcgtaagg ttgggtcgat catcggatgg ggacgcccga cggtgtagaa atcatcgccg
    12241 aggtcaataa tctggtggcc atcggcgtcc agcatcatgc catgttgatg ggtatcgtcg
    12301 gcttccacgc caaggtgccc ggcaagtaat cccgccgctt cggcggccag cgtaccgccg
    12361 gtatacaaac cgcaaataaa tccgctgctg acaggtgcta ttgcgttacg tcgcgccgtg
    12421 acgcgtgaaa gcaggcaagc caggcgtgcg gcctcatcca gcgaggaggc aaaccagaca
    12481 ttctcgtcgc gggccaccgc cggggtataa cctaaaaaca gcgccaccgt cggtttgccg
    12541 gttgctttca tggcattaac aattttcaga cgcacagctt cggcaggcgg ttttgaaaca
    12601 aatgccagca cttcgctttt ctcgtctgca ctgagcattt ccagcgccgt cagcgcactg
    12661 atgccgccca cttcacggct gaggtcgcgc ccgccaaggc caatcgcgtg agtaattccc
    12721 tcccctgcca gcgcaatctg cgaacacagt tcctgaatcc cggtaccgga agcgccaatg
    12781 acgccaatat tgccttccgg catcacgtta gcaaaagcca gcggcgtgcc ggcaatcatc
    12841 gacgtaccgc agtccggccc catcaccagc aagccttttt cccgcgcgcg gcttttaagt
    12901 tggatttcat cttccagcgt gacgttatcg gagaacatca tcacgttgag gttgcgatcc
    12961 agcgcctggt tcgccagctc cgccgcatac tcgccagcca ctgaaatcag cgccagactg
    13021 gcgtcgggta atttctgaca ggcactgtcc cagcgacgca cctgcgtcaa ggcctggctg
    13081 ctgcctgacc cctgcgccag ttgttttagc gcctcttcaa gctgctgcat aatcgcctgc
    13141 gcgatccccg catccgccgc ttcgctacga attgtcacgc aaatatcgtt cgacgtggcg
    13201 ttattaaaat cgtcatgcca gaaacctgtg gtatctaata acgctttatt ggcgggcgta
    13261 cccatcatta cggaaacatc atcaacattt tctgattcgc tgagttttcg tgaaataatc
    13321 attaaactga ccgaatcctg aaaacaccct tttttaataa aggcgtggat cataacaact
    13381 ccttaaaaat tgcacccatg gcaattgcac gagcagtgtt aatgtggcga ttacggtatc
    13441 gatgacaacg ggtaaataag gtgatgaata tcacacaggg attattaaca aaagaattca
    13501 aataaccgcc atgctattac gtgttatttt ttaataagcg tggttgtttt aaaaaccagg
    13561 ttaaaagaga gcggattatc aaataaattc taactatatt tttttgcctg tctggatcac
    13621 ataatccaga tattttccct gtatgttaat tgcagtcatg cttcacaccg tcgttaaaaa
    13681 ggaagacaga tgaaaatcag tcgggaaaca ctccaccagc taatcgagaa taaactctgc
    13741 caggctgggt taaaacgtga gcacgctgca accgtggctg aagtattggt ttacgccgat
    13801 gccagaggga tccactctca tggcgcggtg cgcgttgaat actacgcaga acgcatttca
    13861 aaaggcggca ccaaccgcga accggagttt cgtcttgaag aaaccgggcc gtgctcggca
    13921 attttacatg ccgacaatgc cgccggacag gtcgcggcga aaatgggtat ggaacatgcc
    13981 atcaaaaccg cccagcaaaa aggcgttgcg gtggtcggta tcagccggat gggtcacagc
    14041 ggcgcaatct cttattttgt gcagcaggca gcccgcgctg gattaattgg catttcgatg
    14101 tgccagtccg atccaatggt ggtaccgttt ggcggcgcgg aaatttacta cggtactaac
    14161 ccattggcct ttgccgcgcc gggagaaggc gacgaggtcc ttacctttga tatggcgact
    14221 accgtacagg catggggaaa agtgctcgac gcccgctcgc gtaatatgtc tattccggat
    14281 acctgggcgg tcgataaaaa cggtgcacca acaaccgatc cgttcgcggt acatgctctg
    14341 ctccccgccg ccgggccgaa agggtatggc ctgatgatga tgattgacgt cctctcaggc
    14401 gtcttactcg gcttaccgtt cgggcgacag gttagttcga tgtatgacga tttacacgcc
    14461 gggcgtaatt tggggcaatt acatgtagtt attaacccga actttttctc ctccagcgaa
    14521 ttattccgtc aacatcttag ccagaccatg cgcgaattaa atgccattac ccccgcgccc
    14581 ggttttaatc aggtttatta tcccggacag gatcaggata ttaaacaacg caaagccgcc
    14641 gtcgaaggca tcgaaattgt tgatgatatt taccagtatt taatttccga cgcgctttat
    14701 aacacgtcat acgaaacgaa aaatcccttt gcgcaataat tattgagaca ggactttctt
    14761 atgattacac attttcgtca agccatagaa gaaacgctgc cctggctttc ctcttttggc
    14821 gctgacccag cgggtgggat gacccgttta ctttattcgc cggaatggct ggaaacccag
    14881 cagcaattta aaaaaagaat ggcagcaagc gggctggaaa cacgtttcga tgaagtgggg
    14941 aatttatacg gtcgcctgag tggcaccgaa tatccacagg aagtggttct gagcggttcg
    15001 catatcgata ccgtggttaa cggcggtaac ctcgacgggc aattcggcgc gctggcggcg
    15061 tggctggcaa ttgactggtt gaaaacgcaa tacggcgcgc cgttacgtac gcttgaagtg
    15121 gtggcgatgg cggaagaaga aggcagccgc ttcccgtatg tcttctgggg cagtaaaaat
    15181 atctttgggc tggcgaatcc tgacgacgtg cggaatatct gtgatgccaa aggaaatagt
    15241 tttgtcgatg cgatgaaggc ttgcggattt actctgccga acgccccact aactccgcgt
    15301 caggatatta aagcctttgt cgaactgcat atcgaacagg gctgtgtgct ggaaagtaat
    15361 gggcaatcaa ttggcgtggt gaatgcaatt gtcgggcaac gtcgctatac ggtgacgctg
    15421 aacggcgaat caaaccatgc aggcaccacg ccgatgggtt atcgtcgtga tacggtttac
    15481 gctttcagtc gcatttgcca tcagtcgatc gaaaaagcga aaaagatggg cgacccgctg
    15541 gtgctgacct ttggcaaagt agagccgcgc ccgaatacgg tgaatgtggt gccgggtaaa
    15601 accacgttca ccattgattg tcgtcatacc gacgccgcca tcctgcgtga tttcacccaa
    15661 cagttagaaa acgacatgcg ggcgatttgc gatgaaatgg acattggtat tgatatcgat
    15721 ttatggatgg acgaagaacc cgtgccgatg aataaggacc tggtcgccac cctgacagaa
    15781 ttgtgtgaaa gtgaaaaact gaattaccgg gtgatgcaca gtggtgccgg gcatgacgcg
    15841 caaattttcg cgcctcgcgt accaacctgc atgattttca ttcccagcat caacgggatc
    15901 agccataacc cggcggaacg caccaatatt accgaccttg ccgaaggggt caaaacgttg
    15961 gcaatcatgc tttatcaact tgcctggcag aaataaggag tcataaatgg gatatttaaa
    16021 taacgtcacc ggttaccgcg atgatttact ggctaaccgt gcgattgtga aacacggtaa
    16081 tttcgcactg ttaaccccgg atggtctggt aaaaaatatt attccgggct ttgaaaattg
    16141 tgacgcgaca atcctctcca cgccaaagct gggtgcctct tttgttgatt atctggtcac
    16201 actgcatcaa aacggcggca accaacaggg cttcggtggc gaaggcattg aaacgttcct
    16261 gtatgtgatc tctggaaata tcactgccaa agccgaaggc aaaacatttg ccttaagcga
    16321 aggtggctat ctttattgcc cgccaggctc cttaatgacg tttgttaacg cccaggccga
    16381 agacagccaa atctttttat ataagcgccg ctatgttccg gtagaaggcc atgcaccgtg
    16441 gctggtttct ggcaatgcca gcgaactgga acgcattcac tatgaaggta tggatgatgt
    16501 gatcctgctg gattttctgc ccaaagagtt aggttttgat atgaacatgc atatcctctc
    16561 ttttgcacca ggtgccagcc acggttatat cgaaacacac gttcaggaac acggtgccta
    16621 tattctttcc ggtcaggggg tttataacct cgacaatagc tggatcccgg tgaaaaaagg
    16681 cgattacatc tttatgggcg cttattcttt acaggctggt tatggcgtag ggcgtggtga
    16741 agcgttcagc tatatttact cgaaagattg caaccgcgac gtagagattt aagtcatacc
    16801 actcgcctga ttatttttag ctatgttgga ttttgccacg gtttataccg tggctttttt
    16861 tgtaacattg cctgatgcgc ttcgcttatc aggcctacat gatctctgca atatatagaa
    16921 tttgcgcgat tttcaggaag gatatggtac tcatgccgca tccggcatga gtactgcact
    16981 cttatgcgat ataaatcgca tccgctttaa aggtaagggt tagtttttaa ttccctgacc
    17041 tattttaatg gcgcaggcaa tatttcgcgc gctgttgaag agattggttt caccgctggc
    17101 gagcacttcg gctaaaggtg ccagacgagg caaaatgctg aataccgcgt caatgccgta
    17161 ctggtgcacc acttccacgc catcacccaa tacgccagca atcccaatca ccggtacatt
    17221 aaactgcttc gccaccgacg ccacacccag cggcgcttta ccgcctgccg tttgcgagtc
    17281 gatgcgccct tccccggtaa tcaccagtgc tgcgccctgc actgcctgcg caagattgac
    17341 cgcattcaac acaatctcaa tacctggttt aatatccgca ttgagaaata ccgccgccgc
    17401 aatacccata ccgcccgcag cgccgccgcc cgccatctgg cagacgttga tttcagtttg
    17461 ctgttgtaaa acacgggcgt aattttgcag cccctgttcc agctcttcga ccatttccgg
    17521 cgttgcccct ttttgcgggc caaacaccgc cgccgcgccg cgtgccccta ccagcgggtt
    17581 atcaacgtca catgctactt caatatggca attcgccaga cgcggatcgc attcatccat
    17641 ctcaatgctt gccacgcgcg ctaaattacc gccgttagcc accagcacct gaccgtcggc
    17701 atcaaggaaa cgcacaccga gcgcctgcgc catgcccata ccgccgtcga ccgtcgcact
    17761 gccgccaatg ccgagaataa tatggcgaat gccgttatcc agcgcatgac gaattaactc
    17821 ccccgtacca aaactggagg ccagcaacgg attacgcttt tcaggcgcga ccagcatcag
    17881 gccacttgct gccgccatct caatcaccgc cgttttcccg tcgccggtaa ggccataaaa
    17941 agcattcact gtttcgccca tcggcccgca gacttcaagc gtcacgatgt tgccgcccgt
    18001 cgcggcgacc atcgcatcca ccgttccttc gccgccatcc gctatcggca aacagatata
    18061 gtgcgcatcg ggaaagaggg tcgaaaaccc ggctttaatt gcctggcaac atttttctgc
    18121 acttaagctc tctttaaaag agtctggcgc aatgacaatc ttcattatca atcctttttc
    18181 aacaaattat tcgatgcgtt cccacggaat aagattatcc ataagaacag ccagtaaaat
    18241 cccgaccaat aagccgttac tgagcagagg acgcagcgtt aatggaaggt cttgcagata
    18301 cacaggcggt aatgccatta aaaatatgcc gacaaataac ggcaatgcga gtcgataaat
    18361 attgcgagcg gtaaaggtta tttgctggct aaacaccagc gcggaaaaga gtaaaggcag
    18421 ataagaaacc agcatgaccg cgctactcac gggtaaaggg atactgcaaa acagtcgtgt
    18481 gagtgccgga accagcgcca ccagcaggca aataacgctg ccataaataa acgaacgccg
    18541 cgtgtaatcg ccagtctggg ttaataaacc aatggatgaa acaaacggtg aaaatggaat
    18601 taccgccagc ggtacggtta tcagcgtcat aaatccggtc gccacaaagc tacgacgata
    18661 acgcgtattc cccgcgccct gctgcggata aaaaacatcc gtgccccgaa tcgcaccgta
    18721 ggtattgctg atattcacca gacctgtaat caccgccgtc agaataattc ccggcgacaa
    18781 agcaccgcca ctgccgagcg ggaaccactg ccagtgcaac tcaccggaga gcgaatgcga
    18841 agaaggaaag caaaagtacc acaacaacca gccggttatc gtaccgacca gcaggccata
    18901 acgggcaaaa cgttgcggca gaaagataat catcgccagt accaggcaca tcaccgccac
    18961 cgagagcgcg aacggtggta actgaatttt aaaatttggg tcggctatgc caaacggcag
    19021 cccgagcata cctttgaaaa agatagtggt cagctgcgcg cccagcatca acataaacaa
    19081 gaccatcacc gacggcgtaa ataaccgtgc caggcgatgg cctaatccgc taaaaccaat
    19141 caacatcgtc agcacgccgg agagtgcaat ccccaccgcc agactggtag cgatatcgtt
    19201 gatcggtgtc ccgcgcgatg cttcgccaag ggtgatagta aggatggttc cccaccacag
    19261 gccacccggc ccttccataa tagcgcgacg atgaccgcag aacgcctgag cgaagcaggc
    19321 cagtgcggta gcaagaaaag catattgcgt gagcgtaagc aggctacttt gcggcaactg
    19381 aaaagcagaa agtagcgtag gaggaaccac aaccgtgttg caaaaaataa agaaaaacca
    19441 ctgaaatcct gataacaggc tttcgcggct gactgcaaaa ttgaacataa acacacctta
    19501 accggagaat gcccgccgca aggacgggca ttgcaggggc cagattactg ctgatgttta
    19561 aggataaatt gacctttcgg cgcaacaggg aagccctgtt caatgtcgta aatcacatca
    19621 ccacgtaaga tggttttcgt gatacgcgcg ccaatggtac ggccaacata cgggctgact
    19681 ttgtggcgat attccagatc gtcattggta agaacatagc tgctattcgg ctgaatgaag
    19741 acgaagtcgg catcttttcc tggggcgata cggccttttt gctgcagacc gaaaatatct
    19801 gctgcgttag tcgccattaa tttgccgaac attggcagag acataccgcg tttctgtacc
    19861 gcttcatcga acatcacgtc catgcagctt tgcagaccgg cgataccgcc ccatgctttc
    19921 atgatgttac cggctttcat ttccggcggg catggagagt ggtcggaaac caggcagtcg
    19981 atttcaccgt taaacagttt ttcccacatg cctttctggt tttccagatc gcggatcggc
    20041 ggtgaacact tcgccagagt accgatttct tcgaactgat cggtatccag tacaaagtaa
    20101 tgcgggcagg attcacaagt aacgtcctga ccttcctgac gtgcacgagt cacttcctca
    20161 acaccttccg ggctgctgac gtggcaaacg tgcagacggc aaccagcaac tttcgccaga
    20221 tacagtacgc ggcgaattgc ttccacttcg gtaaataccg gacgcgaagc cacatagcca
    20281 tgagcggtta cgcgaccttc acgcttcgct tcttcgccca gttcgtcaca aatcagcgcg
    20341 ttttcgcagt gcaccagcac cggctgaccc agttcgccca gcttctgcgc acctttgaag
    20401 aactgccagt cgtttacatc acggaagtcg ttgtcgatac cgcgatcgcc acaggtcgca
    20461 acgaagcatt tgaagccgac aacgcccact tcatccagct catgcagccg gtcgatgttg
    20521 taagacacca ggccaccgag ttgtgccgca tcaatagtca gcttgccttt agcggcatcg
    20581 aacttcagtt caattgaagc gcggtcaacc gttgcaggca gctggttgag cggcatttcg
    20641 atcatggtgg tgataccacc ttttgccgct gcgcgagtac cggtttcata accttcccag
    20701 tggctacgac ccggttcaga aatatgggtg tgcacatcaa ccatgcccgg cgaaaccacc
    20761 agaccagacg catccataac ttcttttgca tcgcccagat cctgaccgat agcagcaatt
    20821 tttccgcctt taacggcgat atctacaacg cgagcttcgt tttctaaaat aacggtgccg
    20881 tttttaatga ttaaatcaaa agacataaca aactccttgt aaaatacaga ttggttttta
    20941 actcaatgtt caatatcggg attaattaac ctatggtttt ttgctctcct gttttttctg
    21001 ctgttgtacg tttctttaat aaggcgtagg ccgcaaaggc gacgatgacg ccgacaaacc
    21061 atgaaacacg cgataacggt tccataaagt gaataaactt accgccaaga gataaaataa
    21121 cggccaccag agttactgaa aacgcagtga ggttaaaacc gttatcgtaa tatttataat
    21181 cgccaggtgc ggtatacagt tcatcaagat taatttgtcc gcgcatcacc acaaaataat
    21241 gcgccatcat gacaccaatt accggaccaa gcattccgcc gataatatcg aggaaaagat
    21301 aaatgctgtc ctgattttcc attaatttcc acgggcagat cagcaagctg ataatactgg
    21361 caatcagtac gccgttttta taggtcagtt ttgtcggtgc aatggtggca atctgataac
    21421 cggctggaat aatattaccg gtcgcgttag tggagatagt tgtcatcaga ataaccagta
    21481 ccgcaaagaa cgaggcgaac aggctgtccc aacgctgaac aatatccagc acgttccagg
    21541 tatcagcgcc gtagtgaata ctggctccgg caataataca taccccggcg accgcaaaca
    21601 gaatataggc cacaactaaa cccagcgttt gccccagcgc ctgctcacga aacgagtggg
    21661 cgttttgcgt aaagtcggat gcgctcaccg ccggtgccgc ccagaccgca actaccgcgt
    21721 taatcaccac caggaacagg aagccaccgt tttctgcttt ctgaataccg ctcggaatgt
    21781 agtcaaagat tggaccgatc ccgaccagtg aaatcgccca aatcgccata ccgccgaaaa
    21841 cgatatagat gcacgggtta agaatggcag tgaatttatt taaaactttg ccaccgccaa
    21901 aacctatacc aacgttgacc agccagaaga ttaagaaagt aattaagccc ggtagagaaa
    21961 ggcctaacag agtgaaatca ccaccgagag ttaaaaatcc cggccagatt ttgccaatca
    22021 gaatcaagca ggccagtgac cccgcgtaac attgcaaacc aaaccacatg atggcggcaa
    22081 ttccgcccct taataatccg ggaaacagtg caccacgtac accgtaagaa gcacgcagga
    22141 tcatggcaaa aggcacaccg tatttactgc ccgcagcacc gtttaatacc attaccgcgg
    22201 caatgaaaaa ggcgctgagg ataattgcca gcataatact aaaggtagac aatccgagaa
    22261 taaaaaagcc gccgaccatc acataattgg gaacgttatg aaccgaaccc atccataagg
    22321 taaaatagtt aaatgttttc caggtccgct ggctttgcgt tttcggcaat agatcttcgc
    22381 tatagccgcg ttgctggaat agttttctct gatgttccat aaatccctct taatttcacg
    22441 tatcgaataa cgttgtttta atctagctat gaggaacaaa taaaataaaa atattatttt
    22501 taatactgaa gcaacatctt tggctgcttt cttttttaat attgctggcc gattcatttt
    22561 ctgatatcgg ccatttttat tgcgggtatc aggccagttt atggttagcc attaattcca
    22621 gcgcctgcac taacgcagag tgatccaact ggctgccacc gtttgccgca caggtattaa
    22681 ataactcctg gcaggtcgca gtgtttggca ggttcagcgc aagtgctttc gcactttgca
    22741 gtgccaggtt gagatctttc tggtgcagag cgattttgaa gcccggatta aaggtgcgtt
    22801 taatcatacg ctcgccatga acttccagaa tacgtgagga agcaaagccg cccatcagcg
    22861 cctggcgcac acgtaccggg tccgcaccgg cttttgaagc aaatagcagg gcttcagaaa
    22921 ccgcttcaat attgagcgcc acgataatct gatttgccac tttgcaggtt tgaccatcgc
    22981 cgttaccgcc cacgagggtg atatttttac cgagcagttc aaacagcggt ttaacacgtt
    23041 caaataccgc ttcatcaccg ccaaccataa tcgacaacgt cccttcacgc gcaccgattt
    23101 caccgccgga gactggcgca tcgagataat cgccgcccag ttcattcacc tgacgagcga
    23161 aacgcttagt ttcaatcggg gaaatggagc tcatatcaac aatggttttg cccttcagcg
    23221 aggctttggt acaaccattt tcaccgaaca gaacttcttc aacctgaggt gtgtccggca
    23281 ccataataaa aatgatgtcc gatgcttccg ttacctggcg agcagtttca acactgacgg
    23341 cacccagtga cagtaattca tcagcaaccg gtccaatggt cgtgacatgt aattgatgac
    23401 cggcacgcgc cagattaatg gccatcggtg tacccataat gcctaagcca ataaatccca
    23461 gtttcataat taacctcttt taaatttcgc ttttcctgaa ttcagacaac acgatcccct
    23521 gccaacttag cggcctaaaa aagcattgtc tgaatagcgt tacgttttaa cggtacggat
    23581 ccatccagcg taaaccggct tccgtggtgg tttggggttt atattcacac ccaacccaac
    23641 cgttgtagtc agaattttcg attaccttaa agagataatc ataattaatt tctccggttc
    23701 ccggttcgcc gcgatgcgga ttatcggcaa tttgcaggtg accaatttta tcagcccact
    23761 gagtcatggt gttggttaat tcaccttcca tccgctgcat atgataaatg tcatactgaa
    23821 ttttcaaatt gcagcaacca acatcatcaa tcaatttcag cgcctgccga gttccggtga
    23881 gatggaaacc aggaatatca aaatggttaa taggttcaat cagtaataaa atatcttctt
    23941 tcatcagcat attcgcggca taacgcaggt tttctacaag cgttgcgtga atctgttcac
    24001 tgctgaaacc agccggcgtt ttaccgacca gacagttaat ttttttatta cccagcgcac
    24061 gggcataacg aatcgctgct gctacgccat cccgaaactc ttcttcacgg ccaggaatac
    24121 aggcaatacc gcgctccccc gccgcccagt cacccgccgg taaattgtgc agcgtatgtt
    24181 cgagtttatt actcgccagc acatgtttta attcttcaat gtcgtagtca taaggaaaca
    24241 taaattcaac gccgcgaaaa ccacactgcg cagctttctc aaaacgggcg agaaaatcat
    24301 attctccaaa taacatcgat aaattagcag agaaacgtaa cattatttat ctcccttatt
    24361 catagtgcat gaagcaggtt tcagtcggtg cgtccgctgc gttatcggcg atatcttcaa
    24421 attccatgac gttatccagt tcgctgccca tcgaaatatt ggtcacacgc tcgagaataa
    24481 cttccacgac taccggtacc cgatattgcg ccattaaggc tttcgcctgt tcaaaggctg
    24541 gcgcaatatc ttccggtttg aagacgcgaa tagctttaca acctaaacct tccgctactt
    24601 ttacgtggtc aacaccgtag ccattcactt cactggagtt gatattctcg aaagcgagtt
    24661 gcacgcagta gtccatgtca aaagcgcgtt gtgactgacg aatcagcccc agataagcgt
    24721 tgttgaccag cacatggatg tacggaatgt tgaactgcgc gccaacagct aactcttcaa
    24781 tcaggaactg gaagtcaaag tcgccagaaa tcgccaccac attgcgtttc ggatcagcgg
    24841 cacaaacccc tagcgcagcc ggaatcgtcc agcctaacgg accagcctga ccacagttga
    24901 tccagtggcg gtctttaaag acatgcagca tttgtgccgc agcgatttgt gacagaccaa
    24961 tggtggtgac ataacaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct
    25021 gcggtttcac cggcacgttg tcgaagtggg ttttgcgcag caaagtgcgt ttacgctgct
    25081 ggcagtcggc gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg
    25141 ccacttcaac cagcagtgtc agcgccgctt tagcatcaga gacaataccg agatccggac
    25201 acagcacgcg accaatttgc gtcggctcaa tatcaatatg aacgattttg cgcccttcgg
    25261 tgtatttctc taccgagccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca
    25321 tgtcagacgc cagcagcgtt gcgttaccgt aacgatgcgc ggtttgcaga cccaccatcc
    25381 cggccatcag ttcatgatcg tccgggatac agccccagcc cattagcgtt gggatcaccg
    25441 gaacgctggt cagttcagca aactgttgta acagtgcagc tgcgtcagca ttaattaccc
    25501 cgcccccggc aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga
    25561 tctgcatacg gctggcagca ggtttgtaga ccggcagcgg ttcgtacatg tcaggatcaa
    25621 actcgatttc cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac
    25681 cagaacgcat caggtgaaat gcctgttgca gcacgcgagg caccagcgcc gcttcacgaa
    25741 ctgtaaccgc cattttgctg accggtttag caattgcttc aatatctacg gcctgaaaat
    25801 cttctttatg cagacgggcg cgcggtgcct ggccggtaat gcacagaata ggaatggaat
    25861 cagcagaagc ggaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca
    25921 gacatacgcc gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaagcac
    25981 cttccacatg acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga
    26041 acggattgat tgcagctccc ggaacaccga aggcggtagt gataccttct ttctccagca
    26101 catacattgc cgcgtcaacg gctctcattt ttgccatttt attcctacct ctatttattg
    26161 gaaaattttt ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga
    26221 cagacattat ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct
    26281 gaccagctca ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat
    26341 ggagatagcg gcaacaacac taccgacatc atcgtaaatt gctgaagcta tgcaattcag
    26401 acctacaaca tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc
    26461 cttcagcaag gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg
    26521 cagaatgatg ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc
    26581 ggaagcatgc agtggcagac gactgcccag tggcgcacac atcctgacca tcgatttaca
    26641 ctctaactga ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc
    26701 gccggaaagt aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt
    26761 atggatgtac gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaattg
    26821 actgtcctga tagacaaaat ccgctgcctg taaaaccttc agcaagcgaa aggtcgtgga
    26881 gagcggcaaa tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc
    26941 caaatattgc agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg
    27001 ctccgcctgt cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac
    27061 ctacaagtga gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt
    27121 cacgcaaaac agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg
    27181 cgatcgatgg tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg
    27241 ttacgatggt aattcactcc ctgttcgccg ttggtgataa acgcccgcag cgttgacagg
    27301 tctggtttgt cgtcacctaa cgccacgacc accacaaaca cctcaccttt catcgggata
    27361 aaggcctgag tacccagcgg atgacgttcg agttcgtgaa tggtcagcgg cagattcgcc
    27421 ggttgcgcgc ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc
    27481 aaatcgtggt aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgcgtt
    27541 tcgattacgt cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc
    27601 atctttttgt ccttgtaaca taattccgtt caaccaccgc cgaccggtca gcttggtttc
    27661 aataacaggc aatctaaaac cttaagagaa caacatcaat acaaaagttg gaatattttt
    27721 ccaaaactag atttagtcca caaattgaac agggttatca tgtaaaaata ctgataaatc
    27781 agaggataag aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat
    27841 ccagaaacct tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca
    27901 gaacgattat gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat
    27961 accggagtag cgctgttttt ccgtacgact cgcagcgtga cgttgacagc ggctggcgag
    28021 catctacttt cccaggccag agactggctg agctggctgg aaagtatgcc aagcgagctg
    28081 caacaggtga atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctac
    28141 aacccccagg ccgtcgccca gttgctggcg tggctgaatg agcgttaccc ctttacccag
    28201 tttcacatct cccgacaaat ctatatgggc gtctgggact cgctattgta cgaaggtttt
    28261 tcgctggcta tcggcgtcac gggaactgag gcgctggcaa atacctttag tcttgatccc
    28321 ttaggatcgg tgcaatggcg ctttgtcatg gcggcggatc atccgctggc gaacgttgaa
    28381 gagccgctaa cagaagcgca gttgcggcgc tttccggcgg tcaatattga agacagcgcc
    28441 cgcaccttaa ccaaacgcgt cgcctggcga ttgccagggc aaaaagagat tattgttccc
    28501 gatatggaaa cgaaaatcgc cgcccatctg gcgggcgttg gcattggttt tttgccaaaa
    28561 tcgctttgcc agtcaatgat cgataatcaa caactggtca gccgggtaat cccaacgatg
    28621 cgccctcctt cgccattgag tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa
    28681 gatattgtga ccttgtttac ccagcgcagg ccggaaatca gcggattttt agaaattttc
    28741 ggcaacccac gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca
    28801 agctgatttg aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg
    28861 ccctgctgat tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcacg
    28921 gcgcaatgcc cgccgctatc aatctgccgt taatgaataa cgatgaacgc gccgccgttg
    28981 gcacctgcta taaacagcaa ggctcagacg cagcgctggc gctgggacat aaactggtgg
    29041 cgggtgaaat tcgtcagcag cgcatggacg cctggcgggc agcgtgcctg caaaatccgc
    29101 aaggtattct ctgctgcgcc cgtggcggtc aacgttcaca tattgtgcaa cgctggttgc
    29161 atgaagtggg gattaattat ccgctggtgg aaggcggtta taaggtactg cgccagactg
    29221 cgattcaggc gaccattgaa ctgtcacaaa aaccgatagt gctgattggc ggttgtaccg
    29281 gctgcggcaa aacgctgtta gtgcagcaac agccgaacgg tgttgatctg gaagggttgg
    29341 cgcgtcatcg cggttcggcg tttggtcgca ccttacaacc acaacttagc caggcgagtt
    29401 ttgaaaacct gctggctgcc gaaatgctaa aaaccgacgc ccgtcagaat ttgcgcctgt
    29461 gggtgctgga agacgaaagc cggatgatcg gttcgaatca cctgccggaa tgtctgcgcg
    29521 agcgaatgac tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgagc
    29581 gcctgaacga agagtatttc ttgcgtatgc atcatgattt tacccacgcg tacggcgacg
    29641 aacagggctg gcaggagtat tgcgaatacc tgcatcacgg actttcggcg attaagcgtc
    29701 ggctggggct acagcgctat aacgaactgg ctgcaaggct ggatgcagca ctgacaacgc
    29761 aactcaccac cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttgaagaat
    29821 attacgaccc gatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg
    29881 gtgagtgggc ggaagtggcg gaatgggtta aggcgcggta atgagggacg tatttgtatt
    29941 agtcgcgact tgatctgaca catggccttg aaaggttgag agttaccggt tttgatatga
    30001 gtgtcgaatc cttatccaaa acaagaggta actctcatgc ttcatactgc caatcccgtc
    30061 atcaaacaca aagccggttt gctcaatctg gctgaagaac tcagcaacgt atcaaaagcc
    30121 tgtaaaatca tgggcgtttc gcgtgatacg ttttatcgtt accgcgaact ggtcgctgaa
    30181 ggcggtgtgg atgcgcagat taatcgtagt cgccgcgctc ctaaccttaa gaaccgtacc
    30241 gatgaggcaa ctgaacaggc tgttgttgat tacgccgtcg ctttcccgac acatggtcag
    30301 caccgggcca gcaacgagct gcgtaaacag ggggttttta tctccgacag tggtgtccgt
    30361 tctgtctggt tactccataa ccttgagaat ctcaaaagac gatactgaac aaattttatc
    30421 aggtgacgtt ccgtaaaaag ttgtatggag ttctcgatgc agtcggcgcg actatcagta
    30481 cccccgctga accctggact atcgggccgc cgtactgggg catgtcaccc tggcgtgccg
    30541 ctggttttcc gctaatggtt tatttcctct ttctttcccg gcaagaattt attttaatcc
    30601 ggaaggatta tttattttca gatagggact cacattaaac acgtaacatt ttaattaatg
    30661 catggctgaa tatgtattac acaatgtcag atatcgtaaa agaccagtgc aattctatgt
    30721 aaactctctg actgttcatt ttatttgttg tttcagggtc ggaactcacc ctcaataaac
    30781 aactgaatac gagtagatat tcatcgtctg agctatatgg ctttacacaa tagccgacat
    30841 gtcttttctc agtcaggaat tttacttttc ctcactacga agttgacgac ttcgatatgg
    30901 gatagactct taattcaagc aattactctg cacaagcatc aggcaatcca gctccaatat
    30961 ttgttataaa ataatgcgta acattaattt caccaacaaa aaaaaggttt acaccatccc
    31021 cttgccctgg tatcttacca tatttacacc acactttttt tgtatctgga tcattctttg
    31081 atacgatttt cgccacttgt tccctggccc tcactgtcaa tgttttctta aacctacccc
    31141 catcatcatt tgttagggaa aaataagaaa aaaataatca tcttctacat ttgtgtatat
    31201 tgatatatcc accgctacgt gcgaaaaaaa atctgggagc catgtaaaaa ctccatcatt
    31261 ttctggtatt gccatatagt ttatgtaatg tttacttaca agagattcat ctttgtataa
    31321 ataaagataa gtaattacgc ataaaacaac aatgattata atagcaaaaa taaatattat
    31381 catctttgat agattacttg agatagccag catcttgtaa agcctttatc gtttttttat
    31441 gctctggatt aatataatca ctacatctat ctgagcaatc tgttgttgat ggacatgtca
    31501 acccatggtc atttacagcc aagtcttttt tattatcttc aatcatttca gaatgggata
    31561 gctttacttt tctgccgtac ataccgaaca gatttaaccc gtaatctcta atctcttttt
    31621 cataacccag ccctttcgcc gatcggctaa caccagcgtc atttaattta gacactcgac
    31681 aaaatgccat acaatggaaa aattgatcag tcccacctaa atttaatctc ttcatatctt
    31741 cataagtatc agataatata tcccatgctc tttgatggtt tagttgatct tttcttctta
    31801 ttagcgctac atctgcggga cttaacccta atggatcaat accattcacc ggattcagcg
    31861 gatacgcata cagactccat cccccctcca gccctatcgg gtcctgagtg atataccgcc
    31921 cctgcaacgg atcgtagtac cggttccggt tgtagtacag ccccgactcc ttatcatact
    31981 gctgccccgg cagccggtac ggctggtgca ggtgatgcgg gttctcttca ttcagctggt
    32041 tgccccactc atcatactcc ccgctccacg caatgtttcc atccgcatcc atcagcgcca
    32101 gcattattta ctatgtagga aattcaaaac acatccattt ttcttattaa tttctataat
    32161 aaatacacca cccgcaactt cataaggtaa ctttgctccc tcaacaaccc aactatctgg
    32221 tagttccgta attaaatatg gtttttcttc ttct
//
LOCUS       DAZFZC010000053        31507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFZC010000053 DAZFZC010000000
VERSION     DAZFZC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.053"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(193..456)
                     /gene="cedA"
                     /locus_tag="ACHKXP_002802"
     CDS             complement(193..456)
                     /gene="cedA"
                     /locus_tag="ACHKXP_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO7647774.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            639..2900
                     /gene="katE"
                     /locus_tag="ACHKXP_002803"
     CDS             639..2900
                     /gene="katE"
                     /locus_tag="ACHKXP_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO7647775.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3158..3907)
                     /gene="chbG"
                     /locus_tag="ACHKXP_002804"
     CDS             complement(3158..3907)
                     /gene="chbG"
                     /locus_tag="ACHKXP_002804"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO7647776.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELVSQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEQGIALRADRQMAFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDAGHRGDRSLEVMCHPAFIDNTIRQSAYCF
                     PRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3920..5272)
                     /gene="celF"
                     /locus_tag="ACHKXP_002805"
                     /gene_synonym="chbF"
     CDS             complement(3920..5272)
                     /gene="celF"
                     /locus_tag="ACHKXP_002805"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7647777.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGQIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5377..6219)
                     /gene="chbR"
                     /locus_tag="ACHKXP_002806"
     CDS             complement(5377..6219)
                     /gene="chbR"
                     /locus_tag="ACHKXP_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO7647778.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVALSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6227..6577)
                     /gene="chbA"
                     /locus_tag="ACHKXP_002807"
     CDS             complement(6227..6577)
                     /gene="chbA"
                     /locus_tag="ACHKXP_002807"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO7647779.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6628..7986)
                     /gene="chbC"
                     /locus_tag="ACHKXP_002808"
     CDS             complement(6628..7986)
                     /gene="chbC"
                     /locus_tag="ACHKXP_002808"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO7647780.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRCADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8071..8391)
                     /gene="chbB"
                     /locus_tag="ACHKXP_002809"
     CDS             complement(8071..8391)
                     /gene="chbB"
                     /locus_tag="ACHKXP_002809"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO7647781.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8690..9028)
                     /gene="osmE"
                     /locus_tag="ACHKXP_002810"
     CDS             complement(8690..9028)
                     /gene="osmE"
                     /locus_tag="ACHKXP_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO7647782.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9230..10057
                     /gene="nadE"
                     /locus_tag="ACHKXP_002811"
     CDS             9230..10057
                     /gene="nadE"
                     /locus_tag="ACHKXP_002811"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO7647783.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10287..11174
                     /gene="cho"
                     /locus_tag="ACHKXP_002812"
     CDS             10287..11174
                     /gene="cho"
                     /locus_tag="ACHKXP_002812"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO7647784.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11134..11709)
                     /gene="ves"
                     /locus_tag="ACHKXP_002813"
     CDS             complement(11134..11709)
                     /gene="ves"
                     /locus_tag="ACHKXP_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO7647785.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFSHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11912..12397)
                     /gene="spy"
                     /locus_tag="ACHKXP_002814"
     CDS             complement(11912..12397)
                     /gene="spy"
                     /locus_tag="ACHKXP_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO7647786.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKVKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12727..13695)
                     /gene="astE"
                     /locus_tag="ACHKXP_002815"
     CDS             complement(12727..13695)
                     /gene="astE"
                     /locus_tag="ACHKXP_002815"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO7647787.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13688..15031)
                     /gene="astB"
                     /locus_tag="ACHKXP_002816"
     CDS             complement(13688..15031)
                     /gene="astB"
                     /locus_tag="ACHKXP_002816"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO7647788.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDTVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15028..16506)
                     /gene="astD"
                     /locus_tag="ACHKXP_002817"
     CDS             complement(15028..16506)
                     /gene="astD"
                     /locus_tag="ACHKXP_002817"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO7647789.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDAGAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVQ"
     gene            complement(16503..17537)
                     /gene="astA"
                     /locus_tag="ACHKXP_002818"
     CDS             complement(16503..17537)
                     /gene="astA"
                     /locus_tag="ACHKXP_002818"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO7647790.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17534..18754)
                     /gene="astC"
                     /locus_tag="ACHKXP_002819"
     CDS             complement(17534..18754)
                     /gene="astC"
                     /locus_tag="ACHKXP_002819"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO7647791.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTLNHRYGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19200..20006
                     /gene="xthA"
                     /locus_tag="ACHKXP_002820"
     CDS             19200..20006
                     /gene="xthA"
                     /locus_tag="ACHKXP_002820"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO7647792.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20173..20883
                     /gene="ydjX"
                     /locus_tag="ACHKXP_002821"
     CDS             20173..20883
                     /gene="ydjX"
                     /locus_tag="ACHKXP_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7647793.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     FFGYSLYILLFIIATLLLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSNTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20888..21565
                     /gene="ydjY"
                     /locus_tag="ACHKXP_002822"
     CDS             20888..21565
                     /gene="ydjY"
                     /locus_tag="ACHKXP_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO7647794.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQQENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRDEVKFKGNASVLPADNTLAT
                     VTFKIAE"
     gene            21580..22287
                     /gene="ydjZ"
                     /locus_tag="ACHKXP_002823"
     CDS             21580..22287
                     /gene="ydjZ"
                     /locus_tag="ACHKXP_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7647795.1"
                     /translation="MMMMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22287..22835
                     /gene="ynjA"
                     /locus_tag="ACHKXP_002824"
     CDS             22287..22835
                     /gene="ynjA"
                     /locus_tag="ACHKXP_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO7647796.1"
                     /translation="MGLPPLSKIPLILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22845..24011
                     /gene="ynjB"
                     /locus_tag="ACHKXP_002825"
     CDS             22845..24011
                     /gene="ynjB"
                     /locus_tag="ACHKXP_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7647797.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQVLLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDVLHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23984..25519
                     /gene="ynjC"
                     /locus_tag="ACHKXP_002826"
     CDS             23984..25519
                     /gene="ynjC"
                     /locus_tag="ACHKXP_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO7647798.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKWL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDIDQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGVRKP
                     ATPLLPGNTLAIFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLKLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFAVGIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25519..26172
                     /gene="ynjD"
                     /locus_tag="ACHKXP_002827"
     CDS             25519..26172
                     /gene="ynjD"
                     /locus_tag="ACHKXP_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7647799.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAEQFSCTGELWLNEQRIDILPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLEGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26239..27546
                     /gene="ynjE"
                     /locus_tag="ACHKXP_002828"
     CDS             26239..27546
                     /gene="ynjE"
                     /locus_tag="ACHKXP_002828"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO7647800.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGLTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27555..28175)
                     /gene="ynjF"
                     /locus_tag="ACHKXP_002829"
     CDS             complement(27555..28175)
                     /gene="ynjF"
                     /locus_tag="ACHKXP_002829"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7647801.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28262..28669
                     /gene="nudG"
                     /locus_tag="ACHKXP_002830"
     CDS             28262..28669
                     /gene="nudG"
                     /locus_tag="ACHKXP_002830"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO7647802.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28635..28907)
                     /gene="ynjH"
                     /locus_tag="ACHKXP_002831"
     CDS             complement(28635..28907)
                     /gene="ynjH"
                     /locus_tag="ACHKXP_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO7647803.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29143..30486
                     /gene="gdhA"
                     /locus_tag="ACHKXP_002832"
     CDS             29143..30486
                     /gene="gdhA"
                     /locus_tag="ACHKXP_002832"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO7647804.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30603..>31507)
                     /locus_tag="ACHKXP_002833"
     CDS             complement(30603..>31507)
                     /locus_tag="ACHKXP_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7647805.1"
                     /translation="TCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAG
                     FKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTF
                     QYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPE
                     QVIALLNKFAVFPSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEEL
                     AWHYIMLASRKAHELFESEEDYQKNSQMGFLYWHICCYRRKLTDAELEACYRYDKQFW
                     EHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         7770 a         8301 c         7770 g         7666 t
ORIGIN      
        1 aaatagctca tcatgattag caaaacttaa ccattttaaa ataaataaac aattaaagaa
       61 aaaagatcac ttatttatag caatagatcg tcaaaggcag ctttttgtta caggtggttt
      121 gaatgaatgt agcaatgaaa tacagaattt caggccatgt aactcccggc aaaaccggga
      181 ggtacgtaat ctttactcag tcacttcccc ttcctggcgg atctgatttg cccaacgctg
      241 ggcggattca ggcacagtaa acgccggtga gcgcagaaat gactctccca tcagtacaaa
      301 cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct ttatcgcatg
      361 ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt tttgctggcg
      421 gagcggtttc ttcattactg gcttcactaa acgcatatta aaaatcagaa aaactgtagt
      481 ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac cgtttccaga
      541 atagtctccg aagcggggtc tggctggtgg tctatagtta gagagttttt tgaccaaaac
      601 agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacat aacgaaaaga
      661 acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg gggatggact
      721 cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg ccaggtgcac
      781 aacctaccgc cccagggagc ctgaaagccc ctgatacgcg taacgaaaaa cttaattctc
      841 tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca
      901 tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg ctggaagatt
      961 ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa cgtattgttc
     1021 atgcacgcgg atcagcagct cacggttatt tccagccata taaaagctta agcgatatca
     1081 ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt aggttctcta
     1141 ccgttcaggg tggcgctggc tctgccgata ccgtgcgtga tatccgtggt tttgccacca
     1201 agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca atcttcttta
     1261 tccaggatgc gcataaattt cccgattttg ttcatgcggt aaaaccagaa ccgcactggg
     1321 caattccaca agggcaaagc gcccacgata ctttctggga ttatgtttct ctgcaacctg
     1381 aaactctgca caacgtgatg tgggcgatgt cggatcgcgg gatcccgcgc agttaccgca
     1441 ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg aaggcaacgt
     1501 ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg gatgaagcac
     1561 aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa gccattgaag
     1621 caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa gatgaattca
     1681 agttcgactt cgatcttctc gatccaacca aacttatccc ggaagaactg gtgcccgttc
     1741 agcgtgtcgg caaaatggtg ctcaatcgca acccggataa cttctttgcc gaaaacgaac
     1801 aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac gatccgctgt
     1861 tgcaggggcg tttattctcc tataccgata cacaaatcag tcgtcttggc gggccaaatt
     1921 tccatgagat tccgattaac cgtccgacct gcccttacca taatttccag cgtgacggca
     1981 tgcatcgcat ggggatcgac actaacccgg ctaattacga accgaactcg attaacgata
     2041 actggccgcg cgaaacaccg ccggggccga aacgcggcgg ttttgaatca taccaggagc
     2101 gcgtggaagg caataaagtt cgcgagcgca gcccatcgtt tggcgaatat tattcccatc
     2161 cgcgtctgtt ctggctaagt cagacgccat ttgagcagcg ccatattgtc gatggtttca
     2221 gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg
     2281 cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc gaactgactg
     2341 acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag gatccatcct
     2401 taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg attttactta
     2461 atgatgaagt gagatcggca gaccttctgg ccattctcaa ggcgctgaag gccaaaggcg
     2521 ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt
     2581 tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg gtcattgtcc
     2641 cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactac ctgatggaag
     2701 cctacaaaca ccttaaaccg attgcgctgg cgggtgacgc gcgcaagttt aaagcaacaa
     2761 tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc tgacagcgct gacggtagtt
     2821 ttatggatga actgctaacg ctgatggcag cacaccgcgt gtggtcacgc attcctaaga
     2881 ttgacaaaat tcctgcctga tgggaggcgg cgcaattgcg ccgcctcaaa tgatttacat
     2941 agtgcgcttt gtttatgccg gatgcggcgt gaacgcctta tccggcctac aaaactgtgc
     3001 aaattcaata tattgcagga aacacgtagg cctgataagc gaagcgcatc aggcagtttt
     3061 gcgtttgtca gcagtctcaa gcggcgcagt tacgccgcct ttgtaggaat taatcgccgg
     3121 atgcaaggtt cacgccgcat ctggcaaaca tcctcactta cacatcccga taactcccca
     3181 accgataacc acgctgagcg atagcacctt tcaacgacgc tgatgtcaac acatccagct
     3241 ccgttaagcg tgggaaacag taagcactct gacggatagt attatcgata aacgccggat
     3301 gacacatcac ctccagcgaa cgatcacccc gatggcctgc gtcatccagc acttgcagga
     3361 acagcgactc actgatctct tcaccgtaga atgcactgct gaatccctgg gtagtgcgca
     3421 ggttgaccgg caaatcaaac gccatctgac gatccgcccg caacgcaatg ccctgctctg
     3481 ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat atgcacatga tgatggctat
     3541 caagatgcgt aggtttgcgt ccaaatagct caatgaaacg caaatactga ctgacaagct
     3601 cctgagtaat ttcttccagc ggtaaagcat cttcttctgc caactgccag atccattttc
     3661 ccagcacacc atcgcgggtt aaccccggca tagctgtcag tggcttaccc atagtaagga
     3721 caaagtgcat ccctatggcc agactcggtt catcacgact caactgcacc gcatggtcaa
     3781 tagcctgccc attcaccagc gccgtcgtcg acgtgacaat cccattgcga caggcctcga
     3841 taatgccgta gttctgacct ttgcttaagc caaaatcatc ggcattaaca atcagtaagc
     3901 gttccataat cagcctcggt taatgtgctt ttttaagctc tgcgatgcag tcggcaaagt
     3961 ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc cagcagctca gcatcgcgat
     4021 cggaatgcac caacggacta aggtttagcg ccagtaacac atcgttaaat tctccgctaa
     4081 gtgcggcgtt actggcagca atctcgaagc ctttaatggt gtgaatcagc cccattactt
     4141 tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc agcttacagg
     4201 tcatttctac tgcccagtct gccggaatat tatcaatctg cccatgatgc gggatattaa
     4261 cgtaatgttc tgcttgcttg tcgttgtaga tagcgttgat cacttcgcat gctgcatcag
     4321 agtaataagc cccaccgcgc tgttccagtt ctttcggctt aactttcagc tcaggatttt
     4381 tatacagctc aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt gcgccgcctt
     4441 tgtagtattc gcccatttca atagccagca tctctttctg cttgaagtaa tacagcagat
     4501 aagaacatgg cagcagattc aacgaacgaa ttaagccctc actaaatggc agatcgaaaa
     4561 tattttttac agaggacgct tttaactgcc ctgacgccac accatcaagc aattcggcaa
     4621 agcgcgactt gccatttacc agcacatcct taatgaacac catatggttg aggccgaaca
     4681 aatcgataga taaatcatcg ctgtctttca gcatcagaac atcgcgaata aacatcttca
     4741 tgccgatcgg aatattacac acgccgataa agcgtttaaa tccggtatga cgataaacgg
     4801 cttcagtgac cattcccgcc gggttagtga agttaatcac ccatgcattc ggacaaagtt
     4861 cttcgacatc ttttacgatg tcaaaaatca ccggaatggt acgcagacct ttaaacaaac
     4921 cgcccgcgcc gttggtttcc tgaccaagat aaccatgact taatggaata cgttcatcca
     4981 gttcacgcgc cggtaattgg ccaacgcgca gttgggtagt aacgaaatca gcatctttca
     5041 atgcttcgcg gcgatccagc gttttataaa gcttcatcgg gacgccagcg ttatcaatca
     5101 tccgttggca gagatcgaaa ataatatcca gtttcgcttt accaccttcg acatccacca
     5161 gccataattc gctgaccggc aattcgtgat aacgcttaat aaatccttcc agtaactccg
     5221 gggtatagct gctcccgcca ccaatagtga cgacttttaa tttctggctc ataatttctc
     5281 ccttcagtac agaatactga tatctggcat atctgccccc ccggacataa ataatccagc
     5341 aacaggacag atatgtgaat tgtcaggtat aacgacttac tgattaaatt cagtcaattt
     5401 cttacgatag ctcttaggcg taaaggacgt taatttctta aacgttttaa taaacaaact
     5461 cgggctacta taaccggcct caaacgcaat atccgtcact gaatagttgg tcatttccag
     5521 ttgttttttg gcaaaattaa tacggatttc attaataatc tgcattggcg ttttgccata
     5581 atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg ccaccatatt
     5641 ctccagcgcc gattcactga actgctcttt atcatgcatc ttttctaccg tacttttcag
     5701 ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac ggttaatgac
     5761 ataaaaagta accatctcaa caaactcttc cagccctgtt tcacggaaat tcaatgaaga
     5821 aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct gcgaagcaac
     5881 aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac tgatcccaac
     5941 gttcaatatg cgcgtggcac caaactcata aaaactttga tggtgcgaac ctaacggaat
     6001 aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct ggaaataacg
     6061 cccggttaat accagagtaa attcataata gtcgtgctga tgcagtccgc tgatactctc
     6121 agttttgtta tagataaaca catggaagtt tttgccatta aacaactgct gttctcgggc
     6181 agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat gccttcagtt
     6241 tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac gtcattaaat
     6301 gatcctgagc gtggaccagc accagactca ctttcatctt accttcgccc gcatcgcctt
     6361 caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac tgatccatca
     6421 tggcttttgc tgcggcaaaa tcgccctgct tcgcctgttt cagtgccgca tacgccaggc
     6481 tgcgcgcttg tccggagttg atgataagcc ccatcaccac ttcttccagc tcttcagctt
     6541 ctgtttgcgt atcgggaatg ttatcgagat ccatcataca tcgttcctct cttttcttac
     6601 cggcacgatt acccgtaccg gcatcgatta aaatttcagg gcgttagcga tatcttcttc
     6661 gctctcttct ttatcaatcg cattttgtgc tttgttagcc accacaacaa agggcagata
     6721 aattaacgtt gcgatgccaa ggttgaagag tgcgaccagc aatgcggcga cgctaccgtt
     6781 ggtattaaag aaggctccca gaccggttgg catggtccac ggtgcaatat tggtcaccgg
     6841 aggaataatg cccatgtagt acgctgcgag ggtgattgcc gccagaatcg gttgtaccag
     6901 tacaaacggg ataaacatca ccgggttcat gataattggc agaccaaaca gaatcggttc
     6961 gttaatctgg aagatgccgg acggcagcgc cagttttgcc acctgacgat aatcagcaca
     7021 gcgagaggcg ataaagatag ccaggatcag gcctaaagtc gcaccactgc ccccaaggaa
     7081 aataaaggag tccagcatcg gcttggccca gatatggaag gtcttaccgg ctgccagcgc
     7141 cgcttcgacg gaaccatatt gctgataggt cgcgatattt tccagcgccc acggcgtcat
     7201 tatgccgttg tccagtgcgg tcagcgccag cgcgccatga ataccgaaga accagagcag
     7261 tggaacaaag atcacatagg cccagcccac cacgctaccc aacgatgcca gtggagttga
     7321 gatggtatcc ataatgatct gatggaagtt ggtgccccag gtattcaacg cccaggcaat
     7381 aatccccatc acggaaagaa taataaagcc gggaattaat gccgagaagg aacgcgatac
     7441 tgaagcaggt acgctgtcgg gcagtttaat gacccaattg cggcggacaa taaaggtaaa
     7501 catttctgcc accaccaggc caataataat cccggagatg atattcgccc cacctaacca
     7561 gtttgcgcca accgcatagg cctcaccgac actatatggg gtgacggtca taaatgctgc
     7621 aacggataac aacccagccg ccagcgcatc gactttacgc tcttctgcca gcgccatgcc
     7681 aataaagaac ggtgccatta aagacattat ccctaatgtt ccgttatata cgttgccgcc
     7741 aatacctttc agaccattaa gtgtttcaat ggttgaggcg tcgaggcgaa tacctaagga
     7801 ataaaaaaac gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc
     7861 ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg
     7921 ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt
     7981 actcatagaa aaataccctt aaaaaccgca atttaaatat tgcggtattg atttatgaaa
     8041 taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt taatcgctgc
     8101 aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac
     8161 ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg
     8221 ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc
     8281 ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa
     8341 agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc
     8401 ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct tccatgctct
     8461 gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtatagac
     8521 ctgagcaata gtatttcttt gaccttataa attgtctctc cttgacctgc ggttatgacc
     8581 ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcagac caaaaaaaac
     8641 cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaagtgat tacttcgcag
     8701 cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg acatgtccgg
     8761 tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc aggatgtagg
     8821 tctggcaagt accacgagca tggatcatgc tcacttcaga cgaaggttta cccgcaattt
     8881 gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc tgtacaaact
     8941 ggtctttggt acgatcataa gccgtacaac ccgccagcat ggttaatacc gccgctgcac
     9001 tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag cgtgttagat
     9061 aagcctggaa tacattgggc gctttttcaa gcccgtgaac gaaacggctc cgctttcaga
     9121 ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc
     9181 aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa tgacattgca
     9241 acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag aggaaattcg
     9301 tcgtagtgtc gattttctga aaagctacct gcaaacttat ccgttcatta aatcactggt
     9361 gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc agatggcgat
     9421 taatgagctg cgcctggaaa ccggcaacga atcactgcaa tttattgccg tacgcctgcc
     9481 ctatggtgtt caggccgacg aacaagattg ccaggatgcc attgccttta ttcaaccgga
     9541 tcgcgtatta accgttaata tcaagggcgc ggtattggcc agcgaacagg cattgcggga
     9601 agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa
     9661 agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc
     9721 agaagccatt accggattct tcactaaata tggtgacggc ggtacggaca ttaatccgct
     9781 gtatcgtctc aacaaacgtc agggtaaaca gttactggcg gcattagctt gcccggaaca
     9841 cctttataag aaagcgccaa cggccgatct ggaagatgat cgcccttctc tgccagatga
     9901 agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga aaaacgtacc
     9961 tcaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata aacgccgtcc
    10021 gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcacata ttggattgtg
    10081 cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattcg acctataaaa
    10141 ctatgcaaat tcaatacatt gcaggagtcg aataggcctg acaggcgtag cacgtcagac
    10201 ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc ttcccgcctg ttacactgga
    10261 tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg cggctcgaat
    10321 ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt aatgacttac
    10381 ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg ctctatatcg
    10441 gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg gatgaagccg
    10501 ccatgctacg gcaatcccgg cgaattagct ggatctgcac cgcaggcgaa atcggcgctc
    10561 tgctccttga agcgcgatta atcaaagaac aacagccact gtttaataaa cggttgcgcc
    10621 gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca
    10681 aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat aggcgcgcag
    10741 ctttgcaagc attgcagacc atcgctgatg aacaaaaact ttgttatggc ctgctgggac
    10801 tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag
    10861 catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag tctctggagc
    10921 gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa cagcacccgg
    10981 aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt aattcgctgg
    11041 aagacgcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt tataaaattc
    11101 tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg gcgaatgacc
    11161 agcgagccag ttgatttcac tgaacaacag tttaccctgc ggttgtaaaa gccgcagggt
    11221 gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcagtttgt cgcccagttg
    11281 ccacgccccg ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag taaacgtacg
    11341 ttcggcaatt ctgactttcg ccttacagac atccaggcgc gtcataatat tgaaatccat
    11401 cgacatctgc cctgccgtca gtttcgcttt taccacctgg tccgccgcaa aggcaaaagg
    11461 ctgtaacggt tttaaggtat ggctaaagtg gtctgcgctt tcaaggaaca tctcgccgcc
    11521 ttccagcaac gtcactatcc tttccatacc gggaaacaaa gaaaactccc cattcgccgc
    11581 gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga aagtgcaaat
    11641 ttctcgcgtt tcaccggcag cgtttcgcca cagatttacc gacattttac gcatatcaaa
    11701 gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt aaccgtggat
    11761 ggttatcggc aactcgggtg atttacgtta gtggtgatca ggcttttttt acaactgttt
    11821 gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag
    11881 accggcggtc ttaagttttt tggctgaaag attattcagc agttgcaggc attttacctt
    11941 ttgccgctgg acgttctgtc agacgcttct caaaattagc attaaattgc tttttctgtt
    12001 ccggcgtcag gatgttgtaa attttgttct gggtttccat gtgcgccagc atgttagctt
    12061 tgcgctgttc ttccattttt gcgatctgcg cttcagcttt tactttatcg aaggtatcgc
    12121 tggcaatgat gtcatgcatt gcgcggcgtt cttccagcgg cggacgtttc atctggtcac
    12181 gctggccttt catgatttcg cggatctgct gtttctgcgc gtcggtcagg ttcaggtctt
    12241 tgaacatcat gtcctgatgc ggaccgaact tgcctttgtg gtgcatcatc ggcttcgcgt
    12301 cagccggtgc tgcggtagtg gtgtctgcgg catgggccag gttagccgcg ccaagagcca
    12361 gggtagaggc aacaaacagt gcagttaatt tacgcatatt ctatatcctt cctttcagtt
    12421 atttattacg gctttcttaa gtagcgtgcc gtgttgacga gattaacttt actgacttta
    12481 gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa
    12541 ttatggagaa aaaaagaaaa tttgatggag agtgatgaga gaatattaca acacgatgat
    12601 tttgcagaga ttatgaagaa ctataccgga tgactggtga taaataaagc aaataaccag
    12661 gattaatctg tattaattta taagaaagca acttaatacc cgcagaatga tttctgcggg
    12721 taagtattag cttatttttt cgagcattaa tcccgcgcgt aatcccaacg ctaccaacgg
    12781 attagggaat aacacatact ctacatcatg ggttacggta aaacgttcct ctccgtcctg
    12841 cgccagcaat gttcctttct caaacggcat aaaattcagc gtgtcacttg ccatatgcat
    12901 ttcgaaggac ggcgagtggc gagtaatttg cgaaaccacc cgataacgga gcggcggtgt
    12961 tctcacgata ccgacactct caccagatag cagcgcagca attgcgctgg cagttactgc
    13021 aaactggcga agatcgtttt gcccaaaggg caacgctttg ccaagttcca gcgtacaggc
    13081 cagcgcgcca aaatgtctgg cgctgaaatg ggtaaacgta ccaccaggtt cctgatggaa
    13141 caccagcgcc tccagccccg ccgcacccag ccacgtcaga aatttctcgt cccagggaat
    13201 gtcgcgttgc ggtaatacac cgaactgcgg atgcaaggag ccacgaattg cggtatgtag
    13261 atcaaggtgc cagcgcacag attctttgcc ctggtcataa aaatcttcca ggcactgttc
    13321 cagttcgcgc gcccgacagg tttctccgct ttcagcaaat agctgccaac gaccgccaaa
    13381 cattcgattc atatcgctat ggcaataacg tttcccttgc ttcagcgcag gaggattccc
    13441 gaggatcacc agcaaccgcc aacgtaacgg gatctcgccg tgagatatcg cgccaagcaa
    13501 cgcgtccagc atctccacag gtgccgtctc attaccgtgt attcccgctg aaatcaccag
    13561 tgcgccttgc ggtggcgtta atggcgtcag ttccagcaca ccatcgccca gccagcgcca
    13621 gcgaacgccg ttgatttctc gctcggtgat aaccggtttt ttacccgtta aggtcagagc
    13681 aagaaaatta tccattgccc cctccctcgc gctggaacgg ataaaccgaa ccgagattca
    13741 gtaattggct caatacatcc agtgcttccc gcccttcgcg cagcaattgc gggtcggcca
    13801 gatcggcagc agtaaggcga tcgcggtagt aacgatccac ccagtcattg agcgcattaa
    13861 acagcgtatc gttcatcatc accgccggat tcaccgcccg gcgttcttct tctgtcaata
    13921 ccacccgcaa ccgcaggcac gccgggccgc cgccattcgc catgctttca cgtaaatcaa
    13981 agacttttag ttcgctaatc gggttgtcag cggcaaggag ttcattgaga taaccccata
    14041 ctccggcgtg ttcccgacac tcctgaggca gcaccaacat catggaacca tcatcgcggc
    14101 tcagcagttg gctgttaaac agataggtag acaccgtatc agacacggaa acctgagttg
    14161 ccggaacttc tatcgccata aaaccattga cccgcgcacg caggtttgcc agtaactgtg
    14221 actggcgagc gaacgcctgt tggtggcaaa acagcacctg gcggttactc acggcaatca
    14281 cgtcattatg aaaaacgccc tggtcgataa cgtccgggtt ttgctgggcg aaaatcacct
    14341 gttggggatt cacctgattc agccttgcca ccgcctcgct ggcttcgcga gtctgtcgcg
    14401 ccggataacg ggaaggccgg gtatcattgc cttcttctcg cccgtagaca aaaagttgca
    14461 tacccggttc accgtaatga ccgccgagac gattgtggtt tgccgccccc tcatcaccga
    14521 gcaacgctac ctgtggcaac gccgaatgga cgctaaattt ctcttcgtcg ttaaaaatcg
    14581 cttttaacag cgattcagtg acgggcgctt ccagcgaacg gtgaaattta ttgttcaggt
    14641 tggcaacggt gagatgcact ttgccatcca gcgtatcggc agatggcgcg atcgttgccg
    14701 cattggctac ccacattggc gaagcggaac tgacgctgga aagccagtgc ggtgcctggc
    14761 gtgcaacttt ttccagtacc tgctcatcgc taccactgaa tcccaactga cgcagcaccg
    14821 gaataaacgg acgctcgtgc ggcgggatca cggcctgggg gaatcccgca tcggcaaggg
    14881 ctttcatttt cagtaagccc tgttttgccg cctgtcgcgg gttagacacc tgaaaacggt
    14941 gacgggtaga ggcttcatta ccaaacgaca ggccagcgta atgatgcgtc agccctacca
    15001 gcccgtcgaa attaacttcc caggcgttca ttgcaccacc tcatcggaaa aatccagccc
    15061 ggggttaagc gttgcgggca aagttaacga gtccgactcc aggctcgcca taggccatgc
    15121 gcagtaatct gcggcatacc aagcgctggg gcgatggtta ccggatgcac caatgccgcc
    15181 gaatggcgcg gtactggcag caccagtaag cggtttgttc cagttaacaa tccccgcccg
    15241 cgcctccagc aacagttgat cgaatttttc ccgctcgggg gaaaccagac cgcaagagag
    15301 tccgaagcga gtgttattcg ccattcgaat cgcttcctcg aaagaatcat aacgccagac
    15361 gcgcaaaagc ggcccgaaca cctcttcatc tggtacgcca gcaacgcctg tcatttcaat
    15421 gatccccggc gtcagcaacg atgtctctga ttgtaataag cgcggcgcaa gcagggttcg
    15481 tccgcccatc gcttccagtt gctgccaggc agtaaccacc tgctgtgcgg cctgttcaga
    15541 aatcagcccg ccaataaacg gctgcggttc gtcatcccag ttgcccggcg ttaatcgctg
    15601 gctgacggca accagacgag caagaaacgc atcgccctgc gctccgcttt tgagcaataa
    15661 acggcgggcg caggtgcagc gttgcccggc tgtgacaaac gccgactgaa tggtcagatg
    15721 gacagccgcg tcgatatccg ccacctcatc gataattagc ggattattac cgcccatctc
    15781 aagggcgaga attttctccg gctgaccgga gagctggcga tgcagctggt agccggtatt
    15841 ggcgctaccg gtaaacagca aaccgtcgag atcctccagc gcactcagcg cctgacccgt
    15901 ttcacgcccg ccctgcacca ggttcagtac gcccggcggc aagccagcct gctgccataa
    15961 acgcattacc gcttcgccac tccacggtgt cagttcgctg ggtttaaaga taatggtgtt
    16021 acctgccagc aatgccggaa cgatatgtcc gttcggcaaa tgaccaggga aattatacgg
    16081 cccaaacacc gccagcacgc cgtgcgggcg atgtcgcagg ctcgccgcac cgtccggcat
    16141 ttcactacgc tgctcgccgg tacgaacgtg atacgcctta attgatatcg cgattttatt
    16201 gatcatcgcc gtcacttcgg ttgccgcttc ccagcgcggc ttacccgttt ctctggcaat
    16261 aatcgcggtt aattcggctt tattgctttc cagcaatccg gcaaagcgtt cgacaacgac
    16321 ctgacgttca gccaatgaga gccgcgccca gcgcggaaac gccgcacggg ctgcccgaca
    16381 agcctgttcg acctgagcgg caccggcatc attgccttgc cataacacct cgcccgatac
    16441 cggattacgc ttcacacgcg atgcgccctg gcccgttatc cagtcaccgt taatccataa
    16501 agtcatgctg ttttctcctc tgcgcacagg cgcaccagac gaacgcgatc ccctgcgtgg
    16561 catttgaggg catccagttg tgcggcggtt aaaatcaaac gctcggttgc cggatcggta
    16621 cgcaccagca ccacgcggaa atggtgataa ttttcattgg cgaccaggca ggccggaaaa
    16681 tcgccctgcg caggctgccc ttctgccact tccaccagcc gacttttacg gatggcgcgc
    16741 acgcggtcga tgtcacactc aagcgttggc ccgccgtcga agatgtcgat atagttgcgg
    16801 taacgaaaac cttctttctc cagcaccgcg cgggcaggcg cggtttgcgg atgtacctga
    16861 ccgatgacgt cctgggcttc ctgggataaa aagtgggtat agatcggatg tttcggcatc
    16921 agttctgcaa taaatgcctt ttgcccggtg ccgcagagaa aatcggcgcg gctaaaatcc
    16981 atcgaaaaga agcgtttacc gaggctttgc cagaacggtg aatagccgtg ttcgtcaatc
    17041 accccgcgca tttcagcaac cactttgtca ttaaacttgt cgcgaaaagc cgccataaac
    17101 ataaagcgcg atttcgacag caaatagccg ttgccctctt tgcgccagtc cgggtcgaga
    17161 aacagcgtgc acagctcgct gctgccggtg tgatcgttac tgagaaacag cgtcggcaat
    17221 gcgttataga cattcagctc ttttgaggcg tgaaccaacg tgccgacgcg atagttgtac
    17281 cagggatcgt tcagcccaac cgccacctca atggcacaaa tccccgccac ggtgcctgtc
    17341 tcgctatctt ccagcacgaa cacatagccc tgctcacttt tgggcagttc gccttgccag
    17401 gttttgattg ccctttcgat acgcgccgaa agcgtggctt cattggcggg aagcgacgtc
    17461 aggccgccgc ccgttttgct ggcaagctgc atcagcgccg agacatctga tcgctcaacg
    17521 ggacggatga ccatcatgat gaacctcggc taacaaagtg ttcgcaagca gctgcaaagc
    17581 gatccagtcc ggtcgtcacc tcttcttcgc tgacattgag cgcaggcgca aaacgcacca
    17641 cgttgccacc tgcaatcagt accatcacgc ctgctttcgc cgcttcctga gagatctgtt
    17701 tcgcttgccc ggcgtaatcg gcattcagca cacagccaat caacaaacct aagccgcgaa
    17761 tttcgctgaa caaaccgtag cggtgattaa gggtattaag acgctcaaca aaccagtcgt
    17821 gacgctgttt aacgccatta agcatctctg gtgtgttgat gagctccagc actttgcctg
    17881 ccaccgccga ggccagcgga ttaccgccat aggtggtgcc atgagtgcca acggtcatca
    17941 cgcgggcgca ctcttcggtt gccaacaacg caccgaccgg gaaaccgccg cccagcgctt
    18001 tggcggtagt taacagatca ggcgtcacgc cgtagtgcat ataggcatac agttccccgg
    18061 tgcgcccgac gccggtttgt acttcatcaa aaatcaacag cgcattgtgg cggttacaca
    18121 attcacgcag accttgtaaa aacgcgttgc tggctggcac cacaccgcct tccccctgga
    18181 tgggttcgac aatcaccgca caggtagagt cgtcaatcag cgcgctggca gagttaatat
    18241 cgttatatgc agcatgacga atatccgccg gcagtggcgc aaaatcctgt gaataggctg
    18301 gctgcccacc cgcactgaca gtaaacagcg tgcgaccatg aaacgcattt ttgaacgcca
    18361 cgatgccgct cttatggctg ccgtagcggt cgtgagcgaa tttacgcgcc agttttagcg
    18421 ccgcttcgtt ggcttccgca ccggagttac aaaagaagac gcgatcggca aacgtggcgt
    18481 cgatcaattt tttcgccagt cgcagtaccg gctcgttggt gtaaccgttg ccggtatgcc
    18541 agaatttact cgcctgttcg ttcagcgctt cacgcagttc cggatgcgca tggcccagcg
    18601 cgttcaccgc aatgccaccc gcgaagtcga tatactcttt cccctgctga tcccacaagc
    18661 gcgaaccttc gccacgtacc ggtataaagg gtgccggagc gtaaacaggt atcatccatt
    18721 catcaaagtt ttcacgcgta attggctgag acatagcgac ctctacagta aataattcgt
    18781 tatttatatg ttaataataa gtaatgtttg cgctgtaaat gtagattgca gggttcgtgc
    18841 cagccagtga taaaagtgca taaacggcgg aggctaactg gaaatcaagg agttataacc
    18901 aaaccatatg catttaaagt gcatataaag tgaatacgtt tgcgatgtgg gtgaataaaa
    18961 agaataaaaa acgcaatgtt atgcagaagt aaaatataat tctggaattg tgatcattga
    19021 cgaaatttac tggaaattac tgcgccattc tgacgcagcg cgcaccaaaa gcgggcattt
    19081 tttgcgccat cgttgacatc attaacaacc atcgatcaaa tcacttaaca acaggcggta
    19141 agcaacgcga aattctgcta ccatccacgc actctttatc tgaataaatg gcagcgacta
    19201 tgaaatttgt ctcttttaat atcaacggcc tgcgcgccag acctcaccag cttgaagcca
    19261 tcgtcgaaaa gcaccaaccg gatgtgattg gcctgcagga gacaaaagtt catgacgata
    19321 tgtttccgct cgaagaggtg gcgaagctcg gctacaacgt gttttatcac gggcagaaag
    19381 gccattatgg cgtggcgctg ctgaccaaag agacgccgat tgccgtgcgt cgcggctttc
    19441 ccggtgacga cgaagaggcg cagcggcgga ttattatggc ggaaatcccc tcactgctgg
    19501 gtaatgtcac cgtgatcaac ggttacttcc cgcagggtga aagccgcgac catccgataa
    19561 aattcccggc aaaagcgcag ttttatcaga atctgcaaaa ctacctggaa accgaactca
    19621 aacgtgataa tccggtactg attatgggcg atatgaatat cagccctaca gatctggata
    19681 tcggcattgg cgaagaaaac cgtaagcgct ggctgcgtac cggtaaatgc tctttcctgc
    19741 cggaagagcg cgaatggatg gacaggctga tgagctgggg gttggtcgat accttccgcc
    19801 atgcgaatcc gcaaacagca gatcgtttct catggtttga ttaccgctca aaaggttttg
    19861 acgataaccg tggtctgcgc atcgacctgc tgctcgccag ccaaccgctg gcagaatgtt
    19921 gcgtagaaac cggcatcgac tatgaaatcc gcagcatgga aaaaccgtcc gatcacgccc
    19981 ccgtctgggc gaccttccgc cgctaattta gcagctctcc tggctcaaac tgggtcagga
    20041 gaattaacct tgagaaaaat caacaaactg tcagtaatga tttgttgcct gccgtccttt
    20101 gttataccgt ctctgcgttt ttagttgtct gaccacttct ctattatcaa gtttgatata
    20161 ggaaactcca cgatgaacgc tgagcgtaaa tttctttttg cctgtcttat ttttgcgctg
    20221 gtcatttacg ctatccacgc tttcggttta ttcgatctgc tcaccgattt accccactta
    20281 cagacactca tccgccagag cggatttttc ggctatagcc tctatattct gttattcatc
    20341 attgccaccc tcttgctgtt accaggaagc atactggtga tcgcgggagg aatagttttt
    20401 ggcccgctct tagggacact actctcatta attgccgcca cgctggcctc ctcgtgctca
    20461 ttcctgctgg cgcgctggct gggacgtgat ttactgctga aatacgttgg ccatagcaat
    20521 accttccagg ccattgaaaa aggcattgcg cgtaacggta ttgattttct tattctgacc
    20581 cgcttaatcc cgttgtttcc ttacaatatt caaaattacg cttacggatt aaccacaatc
    20641 gccttctggc cttataccct tatttcggca ctcacgaccc tacccggtat tgttatttat
    20701 accgtgatgg caagcgatct cgccaatgaa ggcattacgc tgcgctttat tttacaactc
    20761 tgtctggcgg gcctggcgct gtttattctc gtccagctcg caaaactcta cgcccgacac
    20821 aaacatgtgg atctgtctgc ttcgcgccgc agcccactta ctcacccaaa aaatgaagga
    20881 tagaacgatg ttgcaacatt attcagtgtc atggaaaaaa ggactggctg cactctgttt
    20941 actggctgtt gctgggctta gcggctgcga tcaacaagag aatgcggcgg caaaagtgga
    21001 atacgacgga ctttcgaaca gccaaccgct gcgtgtcgat gccaataacc atacggtaac
    21061 catgctggtg caaattaatg gtcgtttcct caccgacgac actcgtcacg gtattgtgtt
    21121 taaagatggc tccaacggac ataaatcgct gtttatgggt tatgcgaccc cgaaagcatt
    21181 ttatgaagcc ctgaaagagg caggtggtac gccgggcgaa aacatgacga tggataataa
    21241 agaaacgact catgtcacag gcagcaaact ggatatttcg gtcaactggc aaggggcggc
    21301 aaaagcgtat tccttcgatg aagtgattgt tgacagtaat ggcaagaaac tggacatgcg
    21361 ctttggcggt aatttaacgg cagcagaaga gaagaaaacg ggttgcctgg tgtgtctgga
    21421 tagctgcccg gtcggcatcg tcagcaatgc aacatacact tatggtgcgg ttgaaaaacg
    21481 tgatgaagtt aaattcaaag gcaatgcctc agttctcccg gcggataaca cgctggcaac
    21541 ggttaccttt aaaatcgccg aataaagcca ggataaagga tgatgatgat gcaatcgcga
    21601 aaaatctggt actaccgtat aaccctcatc atcctgttgt tcgctatgct gctggcatgg
    21661 gcgctgcttc ccggcgtcca tgagtttatc aatcgcagcg ttgcggcgtt tgccgccgtg
    21721 gaccaacagg gtatagaacg ctttattcag tcttacggcg cactggcggc agttgtctcg
    21781 ttcttgttga tgattttgca ggccattgct gcaccgctac ctgcgttttt gatcaccttt
    21841 gccaatgcgt cgctgtttgg cgcgttctgg ggcggcttgc tgtcgtggac cagttcgatg
    21901 gccggcgcgg cgctgtgctt ttttatcgcc agagtgatgg gccgcgaagt ggtggaaaaa
    21961 ttaaccggca aaaccgtgct tgacagtatg gacggctttt tcactcgcta cggcaaacac
    22021 accattctgg tctgtcggtt attgcctttt gtccctttcg atccaatcag ctatgctgcc
    22081 ggtttgactt caatacgttt tcgctcgttt tttatcgcca ccgggcttgg tcagttaccg
    22141 gcaactattg tttattcctg ggcgggcagc atgttaacag gcggtacttt ctggtttgtc
    22201 accggactgt ttattctgtt tgccctgacc gtggtgattt ttatggcgaa gaaaatatgg
    22261 cttgaacgcc agaagaggaa tgcctgatgg gtttaccgcc gcttagcaaa attcctttaa
    22321 ttttacgtcc acaggcgtgg ctgcatcgtc gccattacgg cgaggtgcta agccccattc
    22381 gctggtgggg gcggatcccg tttatctttt atctggtgtc gatgtttgtt ggctggctgg
    22441 agcgcaaacg ctcaccgctc gatccggtag tacgatcgct tgtcagcgcg cgcattgcgc
    22501 aaatgtgcct gtgtgagttt tgtgtggata tcaccagtat gaaagtcgcc gagcgcaccg
    22561 gcagcagcga taaactgctg gcagtggctg actggcggca aagcccgctc tttagcgatg
    22621 aagaacggct ggcgctggag tacgccgaag ccgcaagcgt aacgccgcca acggtcgatg
    22681 atgccctgcg tacccgactg gctgcgcatt ttgacgctca ggcgctcacc gaactgacgg
    22741 cattgatcgg cctgcaaaat ctgtcagccc gttttaattc tgccatggac attcccgctc
    22801 aggggctgtg ccgtattcct gaaaaacgtt cttaaggaga gatgatgcgc cattgtgggt
    22861 ggttgctggg attgttatcg ctgttttctc tggcaacaca tgccagtgac tggcaagaaa
    22921 ttaaaaatga ggccaaaggg caaaccgtct ggtttaacgc ctggggcggc gataccgcaa
    22981 ttaaccgcta tctcgactgg gtgagcggcg agatgaaaac ccattacgct ataaacctga
    23041 agattgtccg tctggcggat gccgcagacg cggtgaagcg cattcagacc gaagccgcag
    23101 ccggacgtaa aacgggcggc tcggtggatc tgctctgggt gaacggcgaa aacttccgca
    23161 ccttaaaaga ggccaattta ttacaaacgg gctgggcgga gactctgccc aactggcgct
    23221 atgtcgacac acagctgccg gtgcgggaag atttttctgt gccgacacaa ggtgcggaat
    23281 cgccctgggg cggcgcacaa ctgacgttta tcgcccgccg cgatgttacg ccacagccac
    23341 cacaaacgcc gcaagtctta ctggagtttg ctaaagccaa tcccggcacg gttacctatc
    23401 cgcgcccacc ggactttacc ggcacggcgt ttcttgaaca gttgctgatt atgctgacgc
    23461 ccgatcccgc cgcattaaaa gaagcgccgg acgatgcgac tttcgcccgt gtcactgctc
    23521 ccttgtggca atatcttgat gtgctgcatc cgtatttgtg gcgcgaagga aaggatttcc
    23581 cgccttcacc cgcgcggatg gatgctctgc tgaaagccgg cacattgcgc ctgtcgctga
    23641 cctttaaccc cgcgcatgcg cagcaaaaaa tcgccagcgg cgatttgcct gcaagcagtt
    23701 acagttttgg ctttcgcgag gggatgattg gcaacgtgca tttcgtcacc attcctgcca
    23761 acgcgaatgc cagtgctgcg gcgaaggtag ttgccaattt cctgctctca cccgatgcgc
    23821 aactgcgtaa agcagatccc gctgtctggg gcgatccttc tgttctcgat ccgcaaaaac
    23881 tgcctgacgg gcagcgcgaa tcattgcaat caagaatgcc gcaggatctg ccgccggtac
    23941 tggctgaacc gcacgcaggt tgggtaaatg cgctggaaca agaatggcta caccgttacg
    24001 gtacgcatta atctttttgc tgtgggcgat ggtggcggtg atttatgcac cgctgatccc
    24061 ggcagctctc acgctgatat cgcctgcttt gtcgttgaca cactggcagg cgttatttgc
    24121 cgatccacag ttaccgcagg cattactggc aacgctggtg tcgacaacca tcgcggcggt
    24181 cggggcattg ttgattgccc tgctggtgat tgtggcgctg tggcctgggc cgaaatggca
    24241 gcgtatgtgc gcccgtctgc cgtggctgct cgccattccc catgtggctt ttgccaccag
    24301 cgcccttctg ctctttgctg acggagggct gctttatgac tatttcccgt atttcactcc
    24361 gccaatggac cgatttggca tcggtctggg cctcaccctt gcagtgaaag aaagcgcatt
    24421 tctgctgtgg atcttagcag cagtattgag cgaaaaatgg ctgttgcagc aggtcattgt
    24481 gctggattcg ctgggctaca gccgctggca atgcctgaac tggctgctgt tgccctccgt
    24541 cgcgcctgcg ctggcaatgg cgatgctggc gattgttgcc tggtcgctgt cggtcgtgga
    24601 tgtggcaatt attctcgggc caggtaatcc cccgacgctg gcggtaatta gctggcagtg
    24661 gttaacccag ggcgacattg accaacaaac aaaaggcgcg cttgccagcc tgctgttgat
    24721 gctgttactc gccgcctacg ttttgctgag ctatctgcta tggcgcagct ggcggcgcac
    24781 tattccccgc gtagatggcg ttcgcaagcc tgccacgcct ttattgccgg gcaatacgct
    24841 ggcgattttt ttacccttaa ccggtgtgct gtgtgtggtt ctgctggcga tcctcgcgga
    24901 tcagtcgacg atcaatagtg aagcgctcat caacagcctg acaatggggc tggtggcgac
    24961 attcatcgct ttgctcctgt tactgctgtg gctggaatgg gggccacagc gtcgccagtt
    25021 gtggctatgg ttgcccattt tattacctgc tctgccactg gtggcgggcc agtacacgct
    25081 ggcgctatgg ctgaaactgg atggaagctg gacggcggtg gtctgggggc atctgctgtg
    25141 ggtgatgccg tggatgctgt ttatcctgca acccgcctgg cagcgcattg attcacggtt
    25201 aattttgatt gcgcaaacac tgggctggtc gcgggccaaa atcttctttt acgtgaaatg
    25261 cccactcatg ttgcgccctg tgctgattgc cttcgcggtg ggatttgcag tcggtattgc
    25321 gcagtatatg ccaacgctgt ggctgggcgc ggggcgtttt ccgacgctca ccactgaggc
    25381 ggtggcatta agcagcggcg gcagcaacgg tattctcgcc gcccaggctt tatggcaact
    25441 gctattaccg cttattattt ttgccctgac cgcgttagtc gcaaaatggg taggttatgt
    25501 cagacaagga ctccgctaat gctctgcgtg aaaaatgttt cgctacgttt accagaaagc
    25561 cgcttgctga caaacgttaa ctttacggtg gataaaggtg acattgtcac gttaatgggg
    25621 ccgtctggct gtggaaaatc cactctgttt tcatggatga ttggtgcact ggccgaacag
    25681 ttttcttgta caggtgagct atggctcaat gagcaacgga ttgacatcct acccaccgca
    25741 cagcgtcaga ttggcattct ttttcaggat gcactgttat ttgaccagtt cagtgtcggg
    25801 caaaatttac tgctggcgct accggcgaca cttaaaggga atgcccgacg taatgccgtg
    25861 aatgatgcac ttgagcgttc aggccttgag ggagctttcc atcaggatcc tgccactttg
    25921 tctggcggtc agcgagcgcg cgttgctcta ctacgcgccc ttctcgccca accaaaagcg
    25981 ttactcctgg atgagccatt cagccgtctt gatgtggctc tgcgcgataa ttttcgccag
    26041 tgggtgttca gcgaagttcg cgccctggcg atccccgtcg ttcaggtaac gcacgatctc
    26101 caggatgttc ctgctgatag ttctgttctg gatatggcgc agtggtcaga aaattacaac
    26161 aaactgcgat aacgcaaagt ttttctcaat gcgtcagttc agaatggcgc actcaaaact
    26221 acaatgtcgg gattttcgat gaaacgtgtt tctcaaatga ccgcgctggc aatggcttta
    26281 gggctggctt gcgcttcttc gtgggccgct gaactggcga agcctcttac acttgaccag
    26341 cttcaacaac aaaatggcaa agcgatagat actcgcccca gcgcgtttta taacggctgg
    26401 ccacaaacct taaatggccc ttctggtcat gaacctgccg ccttaaacct ctctgccagc
    26461 tggcttgaca aaatgagcac cgaacagctc aacgcgtgga tcaagcaaca taacctgaaa
    26521 accgatgctc cggtggcgct gtacggtaat gacaaagatg tcgacgccgt caaaacgcga
    26581 ctgcaaaaag caggtttaac gcatatctcc atcctgagtg acgcgctaag cgaaccttcc
    26641 cgtctgcaaa aactgccgca ttttgagcag ctggtttatc cgcaatggct gcacgacctg
    26701 caacaaggta aagaggttac ggcgaaacct gccggtgact ggaaagtcat tgaagcggcc
    26761 tggggcgctc ctaagcttta ccttatcagc catattcccg gcgctgacta catcgatacc
    26821 aacgaagtgg aaagtgaacc gctgtggaac aaagtttctg atgaacaact aaaagcgatg
    26881 ctggcaaaac acggcattcg ccatgacacc acggtcattc tgtatgggcg tgacgtatac
    26941 gctgcagcgc gtgtggcgca gattatgctt tatgctggcg tgaaagatgt gcgcctgctg
    27001 gatggcggct ggcaaacctg gtccgacgcg ggactgcctg ttgagcgcgg aacgccaccg
    27061 aaagtgaaag cggaaccgga tttcggcgtg aagatcccgg cacaaccgca gttgatgctc
    27121 gatatggaac aggcccgtgg actgctgcat cgccaggatg catcgctggt gagcattcgt
    27181 tcgtggccag aatttatcgg tacgaccagc ggttacagct atattaaacc aaaaggtgaa
    27241 atagccggag cacgttgggg acacgctggt agcgactcga cgcatatgga agattttcat
    27301 aacccggatg gcaccatgcg cagcgccgat gatattaccg ctatgtggaa agcgtggaat
    27361 atcaaaccag agcagcaagt ttcattctac tgcggcaccg gctggcgcgc gtccgaaacc
    27421 tttatgtacg cacgcgccat gggttggaag aatgtttccg tgtatgacgg cggctggtac
    27481 gaatggagca gcgatccaaa aaatccggta gcaaccggtg aacgcggccc ggacagtagc
    27541 aaataacatt gcaattactg acgctggagc gacttcagcg tcagataccc actccacacc
    27601 cgcgtaaagg tcgtcatcca gcacaacgca ccgaagatcc acgcaaacca cgcaaaccat
    27661 gcagggaata aacatcccag cacaaacagt aagatcgttt ctgtgccttc ggttaatccc
    27721 cccagatagt aaaacgactt atgtgcataa ccggggttat caatctgatg ttttgctgcc
    27781 agtgcagcaa aggcgagaaa actgctgccc gtgccgataa acgcaaacaa caaccagccg
    27841 cctgccagcg cattttgctc cggtgcagca agaataaagc caaacggcac cagcgcgtaa
    27901 aagagaaaat cgagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc
    27961 gccagcgcac cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc
    28021 agataccagc ccagcgccag aaacggcaga gccagtacgc cgatggcaaa tcccactaat
    28081 gttaaaccat ctggcgtaat gcccggttta tcaagaaccc gcacgcattg atgcaacaac
    28141 ggtttaatcc ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt
    28201 tctgattgag caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt
    28261 tatgaaaatg attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc
    28321 gcaacgcccc gcccagagcg atcaggcggg attatgggag tttgccggtg gtaaagtcga
    28381 gccggatgaa agtcagcggc aggcgctggt gcgtgagtta cgcgaagaac tgggcatcga
    28441 agcaactgtg ggtgagtatg ttgccagcca tcagcgagaa gtttcggggc ggattatcca
    28501 tcttcatgcc tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc
    28561 gctggtctgg tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc
    28621 actattagag gcgtttatgg ctttacgcgc cgccagacca gcggattagt gctaagggtc
    28681 ttgtcatcac gctggcattg cagcagtatt ccctcggctt taattaccgc tccttcagaa
    28741 taatttttat cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa
    28801 ctgaacactt ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt
    28861 gcaattaatg gaaatgctaa tactacggcg aacaatgctc gactcacagg gagctcctta
    28921 acgttattgt ctctgctact gataacggta gccgggcggc aaaactttag cgtctgagtt
    28981 atcgcatttg gttatgagat tactctcgtt attaatttgc tttcctgggt catttttttc
    29041 ttgcttaccg tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac
    29101 aacataagca caatcgtatt aatatataag ggttttatat ctatggatca gacatattct
    29161 ctggagtcat tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa
    29221 gccgttcgtg aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc
    29281 cagatgtcat tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta
    29341 tgggttgatg atcgcaacca ggtacaggtc aaccgtgcat ggcgtgtgca gttcagctct
    29401 gccatcggcc cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc
    29461 aaattcctcg gctttgaaca aaccttcaaa aatgccctga ctactctgcc gatgggcggt
    29521 ggtaaaggcg gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt
    29581 tgccaggcgc tgatgactga actgtatcgc cacctgggcg cggataccga cgttccggca
    29641 ggtgatatcg gggttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc
    29701 tccaacaata ccgcctgcgt cttcaccggt aagggccttt catttggcgg cagtcttatt
    29761 cgcccggaag ctaccggcta cggtctggtt tatttcacag aagcaatgct aaaacgccac
    29821 ggtatgggtt ttgaagggat gcgagtttcc gtttctggct ccggcaacgt cgcccagtac
    29881 gctatcgaaa aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc
    29941 actgtagttg atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa
    30001 gccagccgcg atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa
    30061 ggccaacagc cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa
    30121 ctggatgttg acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgccgaaggg
    30181 gcaaatatgc cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt
    30241 gcaccgggta aagcggctaa tgctggcggt gtagcaacgt caggccttga aatggcgcaa
    30301 aacgctgcgc gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg
    30361 ctggatatcc accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg
    30421 cagggcgcga acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg
    30481 atttaagttg taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc
    30541 attgcagtta cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc
    30601 gtttatgagt atttaacgga tgatgctccc cacggaacat ttcttatggg ccaacggcat
    30661 ttcttactgt agtgctccca aaactgcttg tcgtaacgat aacacgcttc aagttcagca
    30721 tccgttaact ttctgcgata gcagcagata tgccagtaaa gaaatcccat ttgactattt
    30781 ttttgataat cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata
    30841 taatgccagg ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag
    30901 atcatccatg ataattccgc cgcccatatt agcttcgccg aggatttacc ggagctatga
    30961 ttagcgcaat cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata
    31021 acctgctctg gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa
    31081 agaaaatgct ccattaaggc acaataactt tcgctatctt cgatacccca ttgatcctct
    31141 aaagactcgc gtcttttact tatgatatcg atcgagtcaa aaggaagcac atgatattgg
    31201 aaggtatctt tgccaggttc aggctttcgc ggccagaact ccagcgtttc agaccattgc
    31261 ttatgataga atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc
    31321 ggctcaatac ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc
    31381 cgaacaataa gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg
    31441 cctctttcat tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca
    31501 catgtta
//
LOCUS       DAZFZC010000054        31482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFZC010000054 DAZFZC010000000
VERSION     DAZFZC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.054"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(101..3250)
                     /gene="acrB"
                     /locus_tag="ACHKXP_002834"
     CDS             complement(101..3250)
                     /gene="acrB"
                     /locus_tag="ACHKXP_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO7647806.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3273..4466)
                     /gene="acrA"
                     /locus_tag="ACHKXP_002835"
     CDS             complement(3273..4466)
                     /gene="acrA"
                     /locus_tag="ACHKXP_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO7647807.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4608..5255
                     /gene="acrR"
                     /locus_tag="ACHKXP_002836"
     CDS             4608..5255
                     /gene="acrR"
                     /locus_tag="ACHKXP_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO7647808.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5383..8745
                     /gene="mscK"
                     /locus_tag="ACHKXP_002837"
     CDS             5383..8745
                     /gene="mscK"
                     /locus_tag="ACHKXP_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO7647809.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHANEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8957..9118)
                     /gene="rsmS"
                     /locus_tag="ACHKXP_002838"
     CDS             complement(8957..9118)
                     /gene="rsmS"
                     /locus_tag="ACHKXP_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO7647810.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9132..9659)
                     /gene="priC"
                     /locus_tag="ACHKXP_002839"
     CDS             complement(9132..9659)
                     /gene="priC"
                     /locus_tag="ACHKXP_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO7647811.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9729..10106
                     /gene="ybaN"
                     /locus_tag="ACHKXP_002840"
     CDS             9729..10106
                     /gene="ybaN"
                     /locus_tag="ACHKXP_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO7647812.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10259..10810
                     /gene="apt"
                     /locus_tag="ACHKXP_002841"
     CDS             10259..10810
                     /gene="apt"
                     /locus_tag="ACHKXP_002841"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO7647813.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10939..12870
                     /gene="dnaX"
                     /locus_tag="ACHKXP_002842"
     CDS             10939..12870
                     /gene="dnaX"
                     /locus_tag="ACHKXP_002842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO7647814.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQTRPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12203..12267
                     /gene="dnaX"
                     /locus_tag="ACHKXP_002842"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12923..13252
                     /gene="ybaB"
                     /locus_tag="ACHKXP_002843"
     CDS             12923..13252
                     /gene="ybaB"
                     /locus_tag="ACHKXP_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO7647815.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13252..13857
                     /gene="recR"
                     /locus_tag="ACHKXP_002844"
     CDS             13252..13857
                     /gene="recR"
                     /locus_tag="ACHKXP_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO7647816.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13967..15841
                     /gene="htpG"
                     /locus_tag="ACHKXP_002845"
     CDS             13967..15841
                     /gene="htpG"
                     /locus_tag="ACHKXP_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO7647817.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTIADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDASDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16022..16666
                     /gene="adk"
                     /locus_tag="ACHKXP_002846"
     CDS             16022..16666
                     /gene="adk"
                     /locus_tag="ACHKXP_002846"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO7647818.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVNRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16902..17864
                     /gene="hemH"
                     /locus_tag="ACHKXP_002847"
     CDS             16902..17864
                     /gene="hemH"
                     /locus_tag="ACHKXP_002847"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO7647819.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPEMPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17861..18820)
                     /gene="aes"
                     /locus_tag="ACHKXP_002848"
     CDS             complement(17861..18820)
                     /gene="aes"
                     /locus_tag="ACHKXP_002848"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO7647820.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18972..20276
                     /gene="gsk"
                     /locus_tag="ACHKXP_002849"
     CDS             18972..20276
                     /gene="gsk"
                     /locus_tag="ACHKXP_002849"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO7647821.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20409..22085)
                     /gene="ybaL"
                     /locus_tag="ACHKXP_002850"
     CDS             complement(20409..22085)
                     /gene="ybaL"
                     /locus_tag="ACHKXP_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO7647822.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGVAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22323..23543)
                     /gene="fsr"
                     /locus_tag="ACHKXP_002851"
     CDS             complement(22323..23543)
                     /gene="fsr"
                     /locus_tag="ACHKXP_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO7647823.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23761..25413
                     /gene="ushA"
                     /locus_tag="ACHKXP_002852"
     CDS             23761..25413
                     /gene="ushA"
                     /locus_tag="ACHKXP_002852"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7647824.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLDVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25450..25929)
                     /gene="ybaK"
                     /locus_tag="ACHKXP_002853"
     CDS             complement(25450..25929)
                     /gene="ybaK"
                     /locus_tag="ACHKXP_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO7647825.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26133..26927)
                     /gene="ybaP"
                     /locus_tag="ACHKXP_002854"
     CDS             complement(26133..26927)
                     /gene="ybaP"
                     /locus_tag="ACHKXP_002854"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO7647826.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27065..27406
                     /gene="ybaQ"
                     /locus_tag="ACHKXP_002855"
     CDS             27065..27406
                     /gene="ybaQ"
                     /locus_tag="ACHKXP_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7647827.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27722..30226)
                     /gene="copA"
                     /locus_tag="ACHKXP_002856"
     CDS             complement(27722..30226)
                     /gene="copA"
                     /locus_tag="ACHKXP_002856"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO7647828.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKHLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLHNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30488..31420
                     /gene="glsA"
                     /locus_tag="ACHKXP_002857"
     CDS             30488..31420
                     /gene="glsA"
                     /locus_tag="ACHKXP_002857"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO7647829.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEDGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         7688 a         8364 c         8264 g         7166 t
ORIGIN      
        1 ttcgtatgag atcctgagtt ggtggttcaa ttactcctta atgttcgtag gttatgcata
       61 aaaaaggccg cttacgcggc cttagtgatt acacgttgta tcaatgatga tcgacagtat
      121 ggctgtgctc gatatcttca ttcttgcggc taaagcggcg gcgaaccacc acaaagaata
      181 ccggaacgaa gaagattgcc agtaccgttg cggtcaccat cccgcccatt acaccggtac
      241 ctactgcgtt ctgcgcgccg gaaccagcac cagtactgat aaccagcggc ataacgccga
      301 ggataaacgc cagcgaggtc atcaggatcg gacgtaaacg catccgcacc gcatcaagcg
      361 tcgcttcaat cagaccttta ccttctttat ccatcaagtc tttggcgaat tcgacgataa
      421 gtatcgcgtt cttcgccgac aacccaatgg ttgtgagcag gcctacctgg aagtaaacgt
      481 cattggtcag gccacggaag gtggcagcca gcaacgcacc gataaccccc agcggaacga
      541 ccagcataac ggagaacgga atcgaccagc tctcgtacag cgccgccaga cacaggaaca
      601 cgacaatcaa cgaaatcgcg tacagtgaag gtgcctggtt gccggagaga cgttcctgat
      661 aggacatccc cgtccagtca tagccaacac cggtaggcag tttgctcgcc agttgttcca
      721 tcagctccat tgcttcaccg gtacttttac ccggtgccgc ctggcctaag atttccatgg
      781 atggcaggcc attgtaacgt tccagacgcg gcgaaccgta ctcccaacgc gaagaggaga
      841 acgccgagaa tggcaccatc tgaccatcag cagcacgaac ataccagtcg ccgatatcat
      901 ccggcagcat acggtatttc gcttctgaca tgacgtaaac tttcttcaca cgaccgcggt
      961 cgataaagtc gttcacatag ctgccgcccc atgcagcgcc cagagtggtg ttaatgtcgt
     1021 tgatagaaac acccagcgcc tgcgcttttt cctggtcgat atcaatctta aactgcgggg
     1081 tatcttccag accgtttgga cgtacgctgg tcaacatatc agggtgcttc gctgcttctg
     1141 caagcagctg gttacgcgcc tgagtcagtt tttcgtgacc aaggccagcc tggtcaatca
     1201 gctcaaagtc aaagccggtt gcagtaccca gttccacgat tgcgggcagg ttaaaggcga
     1261 aaaccatcgc atctttgatt tgcgagaaag cgcgtgttgc acgcatggta atcgcttcaa
     1321 ctttgttttc ttcgcccgga cgatcggccc agtccttcaa ggaaacgaac gcaataccgg
     1381 tattctgacc acgtcccgca aagccgaagc cgttaacggc gaacaccgac tcaacgttgt
     1441 tcttttcttt ggtcagatag taatgcgtta cctcattgag cactttctgt gtacgttcct
     1501 gcgttgcacc tgctggcagc tgaaccatgg tcataaacac gccctggtcc tcatctggca
     1561 agaaggagct tggcagacgc acgaacagat aggccatgcc gaccacgatg atcagataca
     1621 gcaccaggta acgccccgta ctgcgcagaa taccgcctac gctgtcggtg tagtggtgcg
     1681 tgctcttctc gaacatgcgg ttaaaccagc cgaagaagcc ttttttacct tccccgtgat
     1741 cgcctttggc aatcggtttc agcatggtgg cacaaagagc tggagtcagg atcaacgcca
     1801 ccagtaccga cagcgccatt gctgaaacaa tggtaataga gaactgacga tagatagcac
     1861 cagtagaacc gccaaagaag gccatcggta cgaataccgc cgacagtacc atcgcgatac
     1921 cgaccagagc gccctgaatc tgccccatcg acttacgggt ggcttctttt ggcggcaaac
     1981 cttcttccgc catcacacgc tcaacgtttt ctaccacaac gatagcgtca tccaccaaca
     2041 ggccgatggc gagcaccatc ccgaacattg ttagcgtgtt tatcgagaag ccaaaggcgg
     2101 caaggacggc aaaggtaccg agcaatacca ccggtacggc aatggtcgga atcaacgtcg
     2161 cgcggaagtt ctgcaggaac agatacataa ccaggaacac gaggatgatc gcttcgacca
     2221 gcgttttaac cacttcgtga atagagattt tcacgaacgg cgtggtgtcg tacgggtaaa
     2281 caattttcag acccgacggg aagaacggtt ccatcttcgc cagttcagca cggattgccg
     2341 cagcggtatc cagcgcgttt gcaccggtcg ccagcttgat ccccagaccg gaagccggtt
     2401 ggccgttaaa ctctgcgatg atgtcgtagt tctcaccacc cagctcaatc ttcgccacat
     2461 cacgcagcag tacgcgggaa ccatcctgat tcactttcag caggattttg ccgaactctt
     2521 cagtagaggt cagacgcgtc tgagcaataa tagaggcgtt aagctgttgg cctttcaccg
     2581 gcggcgtacc accgagctga cccgccgcaa cctgggcgtt ctgcgctttg atggcggtaa
     2641 tgacatcaac cggcgttagc tggaatttgt tcagctcatt cgggttcatc catatacgca
     2701 tcgcgtactg tgaaccgaac aactgaacat cacccacgcc cgacgtacgg ctgatggcat
     2761 ctttcatatt cgccgccacg tagtcggaga tatcctcctg cgtcatggtg ccatcggtgt
     2821 tgataacgcc gacaaccatc aggaagctgc tggatgattt ctcaacgctc accccttgct
     2881 gctgaacttc ttgcggcagc aacggcatcg ccagctgcag tttgttctgc acctgaacct
     2941 gcgcgatatc cgcatcagta ccagactcaa aggtcagggt gatctgcacg gtacccgtgg
     3001 agtcactgtt agaggacatg tacatcaggt tatcgatacc gttcatattc tgttcgataa
     3061 cctgtgtcac cgtgtcctgc actgttttcg catcagcgcc ggggtaggag gcggagatcg
     3121 ttactgccgg cggtgcaatc gtaggatatt gcgccaccgg cagtttgagg atcgccagcc
     3181 cccctgccaa catgatgata atggcgatca cccacgcaaa aatcgggcga tcgataaaga
     3241 aattaggcat gtcttaacgg ctcctgttta agttaagact tggactgttc aggctgagca
     3301 ccgcttgcgg cttgctggtt attatcagcg gtaacttctt gtgcttttac ctggacacca
     3361 ggacgcactt tctgcagccc acttattact acgcgatcgc ctgctttcag accttctgtc
     3421 accagccact tatcgccaat agcctggctt gcaacgatcg gacgggtttc cactttgtca
     3481 tccgcgccaa ccaccagtac ggtggcatcg ccacgcggcg tacgggttac gccctgttgc
     3541 gggactaaaa tagcgtttgg attaagccct tcttccagac gtgcgcgcac gaacataccc
     3601 ggcagcagag tgtgatccgg gttcgggaag atagcgcgta gggtgataga cccagtggtc
     3661 tgatcaacgg taacgtcaga gaattccagc gtaccgtcct gcgggaactt aatgccgtca
     3721 ctggtgatca gcgacacttt ggctttgccg ttctcttgtt tcagcgtgcc attcgccagt
     3781 tcctgtttca ggcgcaggaa gtcgttgctg gactgggtca catcaacgta gatcggatca
     3841 agttgctgca cggttgccag cgcagtcgcc tgaccgttct gtaccagtgc gccttccgtc
     3901 acgttcgact taccaatgcg accgctaatc ggagaggtga ctttggtgta agccagattg
     3961 atccgcgcag tttcaacggc agctttcgcc gcagttaccg cagcattcgc ctgttgcgca
     4021 tcagccagag cctgatcgta ctcttgctta ctgatgtact gagtaccgag cagtttctga
     4081 taacgattca ccgtcaattg cgcgatattg gctgcagcct gggctttcgc cagatcacct
     4141 ttcgcactgt cgtatgtcgc ctgataggtc gcaggatcaa tctgatagag agagacacct
     4201 gcttcgatgt cgctaccttc tttgaaatta cgcttcagga taatcccgct aacttgagga
     4261 cgaacttctg cgatccggta ggcactggtg cgacccggaa gctcggttgt gatctgcaga
     4321 ggttcagttt tgactgttac tacgccaacg gcgggcatct gctggccacc ttgttgggcc
     4381 tgtttgtcgt cacatcctgt tagggctaag ctgcctgaga gcatcagaac gaccgccaga
     4441 ggcgtaaacc ctctgttttt gttcatatgt aaacctcgag tgtccgattt caaattggtc
     4501 aatggtcaaa agttaataaa cccattgctg cgtttatatt atcgtcgtgc tatggtacat
     4561 acattcacaa atgtatgtaa atctaacgcc tgtaaattca cgaacatatg gcacgaaaaa
     4621 ccaaacaaga agcgcaagaa acgcgccaac acatcctcga tgtggctcta cgtcttttct
     4681 cacagcaggg ggtatcatcc acctcgctgg gcgagattgc aaaagcagct ggcgttacgc
     4741 gcggtgcaat ctactggcat tttaaagaca agtcggattt gttcagtgag atctgggaac
     4801 tgtcagaatc caatattggt gaactagagc ttgagtatca ggcaaaattc cctggcgatc
     4861 cactctcagt attaagagag atattaattc atgttcttga atccacggtg acagaagaac
     4921 ggcgtcgatt attgatggag attatattcc acaaatgcga atttgtcgga gaaatggctg
     4981 ttgtgcaaca ggcacaacgt aatctctgtc tggaaagtta tgaccgtata gaacaaacgt
     5041 taaaacattg tattgaagcg aaaatgttgc ctgcggattt aatgacgcgt cgcgcagcaa
     5101 ttattatgcg cggctatatt tccggcctga tggaaaactg gctctttgcc ccgcaatctt
     5161 ttgatcttaa aaaagaagcc cgcgattacg tcgccatctt actggagatg tatctcctgt
     5221 gccccacgct tcgtaatcct gccactaacg aataaccctg aatctgactc caggattttt
     5281 cctggacatt ttcgtcgttg ctattctggt tcactgcgtc gtgatattct tgcggtttga
     5341 ctttttcagg tcgttcttca ggttcagaaa ccttcattca tcatgactat gttccagtat
     5401 tacaaacgat cacggcattt tgttttttca gcatttattg cttttgtttt tgtcttgtta
     5461 tgccagaaca cggcgtttgc gcgggcgtca tcgaatggtg atctgccgac aaaagcggac
     5521 ctgcaggcgc aacttgactc actaaataaa caaaaagatc tttctgctca ggacaaactg
     5581 gtgcagcagg atctgacaga tacattagcc accctcgata aaatcgatcg cgtaaaagaa
     5641 gagacagttc agctacggca aaaagtcgct gaagcgccgg aaaaaatgcg ccaggcgacc
     5701 gcggcgttaa cagcgcttag cgatgtcgat aacgacgaag aaacgcgcaa aatcctgagt
     5761 acgctttcgt tgcgccagct ggaaactcgc gttgcccagg cgctggacga tttgcaaaac
     5821 gcacaaaacg atctggcgtc ttataacagc cagctggttt cgttacagac gcagcccgaa
     5881 cgcgtgcaaa atgcgatgta taacgcttcg cagcagctgc aacaaattcg cagtcgtctg
     5941 gatgggactg atgtcggcga gactgcctta cgtcccagcc agaaagtgtt aatgcaggct
     6001 cagcaggcgt tgctgaatgc ggagattgac cagcagcgta aaagcctgga agggaacacc
     6061 gtcttgcagg ataccttgca aaagcaacgt gattacgtga cggcgaacag cgcccgtctg
     6121 gagcaccagt tacaactgtt gcaagaagcg gtaaacagca agcgcctgac tttaaccgaa
     6181 aaaacggcgc aggaagccgt ctccccggat gaagccgcgc gtattcaggc taatccgctg
     6241 gtgaagcagg aactggaaat taaccagcag ttaagtcagc gtctgattac cgcgactgaa
     6301 aacggtaatc agttgatgca gcaaaacatt aaagtcaaaa actggctgga gcgggcgctg
     6361 caatcggaac gcaatattaa agagcagatt gccgtcctga agggcagcct gctgttatct
     6421 cgtatccttt accagcaaca acaaacgctg ccctcggcgg atgaactgga aaacatgacc
     6481 aaccgcatcg cggatttgcg tctcgaacag tttgaagtca accagcagcg tgatgcactc
     6541 ttccagagcg atgcgttcgt caacaaactg gaagaaggtc acgccaacga agtcaacagc
     6601 gaagttcacg atgcgttatt gcaagtggtt gatatgcgtc gcgaattgct ggatcaactc
     6661 aacaaacagt tgggtaacca gctgatgatg gccattaacc tgcaaatcaa ccagcagcag
     6721 ttaatgagtg tgtcgaaaaa cctgaaatcc atcctgactc agcaaatctt ttgggtgaac
     6781 agtaaccgtc caatggactg ggactggatc aaagcgttcc cgcaaagcct gaaagatgaa
     6841 tttaagtcga tgaaaatcac ggtgaactgg gaaaaagcct ggcctgccgt ttttatcgct
     6901 ttcctcgccg gtttgccgct gctgttgatt gccgggctga tccactggcg tctgggctgg
     6961 ctgaaagcgt atcaacagaa actggcttcc gctgtgggtt ccctgcgtaa cgatagccag
     7021 ctcaacacac caaaagcgat tcttatcgat ctgatccgtg cgcttccggt gtgcctgatt
     7081 attctcgcgg ttggcctgat tctgttgacc atgcagctca acatcagcga actgctatgg
     7141 tcgttcagca aaaaactggc gattttctgg ctggtgtttg gcctgtgctg gaaggtactg
     7201 gagaagaacg gcgttgccgt gcgtcacttc ggtatgccgg aacagcagac cagccactgg
     7261 cgtcggcaaa ttgtccgcat cagcctcgcg ttgctgccta tccatttctg gtctgtggtg
     7321 gcagaacttt ccccgctgca tctgatggat gatgtgctgg ggcaggcgat gattttcttc
     7381 aacctgctgc tgattgcctt cctggtatgg ccaatgtgcc gcgaaagctg gcgtgataaa
     7441 gagtcgcaca ccatgcgact ggtcaccatt accgtgctgt cgattatccc gattgcgttg
     7501 atggtgctga ctgccacggg ttatttctac accacgctgc gcctctccgg gcgttggatt
     7561 gaaaccgttt atttggtgat catctggaac ctgctgtacc agacggtatt gcgcggctta
     7621 agcgtagcgg cgcggcgtat cgcctggcgc cgtgcgctgg cgcgtcggca gaatctggtg
     7681 aaagagggcg cagaaggtgc cgaaccgccg gaagaaccca ccattgcact ggagcaggtt
     7741 aaccagcaga cgctgcgtat taccatgttg ctgatgtttg cgctgttcgg tgtcatgttc
     7801 tgggcaattt ggtccgattt gatcaccgtg ttcagctatc tcgacagcat tacgctctgg
     7861 cattacaacg gtactgaagc tggtgctgcg gtggtgaaaa acgtcaccat gggcagtctg
     7921 ttgtttgcga ttatcgcctc aatggtggcc tgggcgttga ttcgcaacct gcctggtttg
     7981 ctggaagtgc tggtgctctc gcgactgaat atgcgccagg gcgcgtcgta tgccattact
     8041 accatcctta actacatcat tattgccgtt ggtgcgatga cggtgttcgg atcgctgggc
     8101 gtctcgtggg ataaactcca gtggctggcg gcagcattat ccgtaggtct tggttttggt
     8161 ttacaagaaa ttttcggtaa cttcgtctcc ggtttgatca ttctgttcga acgtccggtg
     8221 cgtattggcg atacggtaac cattggtagc ttctcgggga cggtaagtaa gatccgtatt
     8281 cgtgcgacaa cgattaccga tttcgatcgc aaagaagtga tcatcccgaa caaagcgttt
     8341 gttaccgagc gtctgatcaa ctggtcgttg actgacacta ctacgcgtct ggtgatccgt
     8401 ctcggcgtgg cctatggctc cgatctggaa aaagtgcgta aagtgttact gaaggcggcg
     8461 actgagcacc caagggtgat gcacgaacca atgccggaag tcttctttac ggcatttggt
     8521 gccagcacgt tggatcatga gctgcgtctg tatgtgcgtg aactgcgtga ccgtagtcgt
     8581 actgtcgatg agctgaaccg tactatcgat cagctgtgcc gtgaaaacga catcaacatt
     8641 gcctttaacc agcttgaagt gcatctgcac aacgagaagg gcgatgaggt gacggaagta
     8701 aaacgcgact acaaaggcga tgacccgacg ccagcggtag ggtaaaaacg aaagggggca
     8761 acatttagtt gccccgagat tgctaacaaa gtgcgcgttg ttcatgccgg atgcggcgtg
     8821 accgccttat ccggcctacg aaaccgcaag aattcaatat attgcaggag cggtgtaggc
     8881 ctgataagcg tagcgcatca ggcagttttg cgtttgcccg caaccttagg ggacatttag
     8941 cgaccccatt tatttctcac ttttccgcct catcatcgcg cgttaatttc ttttcataat
     9001 cacgctttac aatatccagc gcgcgcagca cggtactggc agggatctga ttttcctcca
     9061 gcagcacaat caaatcgaca gccagtttga catcgtcagg ggcattttcc agtgacatac
     9121 tctctccatt gctagcgggt taaacgcgct aacctgtttt cgattttttc cagcgcatgg
     9181 cggcagcgtg ccaggcgcgc ttcataggct tccacttcac gatgcagcgt ttgctgttcc
     9241 acgagatcgg tcacccgcgc caggcgggct ctgcgttcgg caaccatctc acgtagccgc
     9301 cgctcaaaat cctgatgctg aatacgttta cgctgccagc gggcaatttt cggtggcgca
     9361 ctgtcccact cgcgcaatga ccaggcggag gcttcacgcg cgatggcttc cagttgtgcc
     9421 gccagatgtt ccgccagcca ggccacttgc ggcagctgtt gctgctcaac cgcatgacga
     9481 agcgcagcca ggttatcgcc cgcctcatcg agacaagctt gtagtgttgt cgcacgagtc
     9541 tgaaaaagat gcctgtcgaa acgagcactt agcgtggcga actgtgacac cggggcacaa
     9601 cgctgacgca gcgtagcgag ctgtccttcc agtttttcca gcagcagggc ggttttcacg
     9661 ataagacctc aatgaaaatg ataattgtta tgctaaagta gccactctgt aagctgacaa
     9721 cactcaatat gcaacgaatc attttaatca tcattggctg gctggcggta gtgctgggta
     9781 cgctgggcgt ggtattaccg gtattaccga cgacgccgtt tatcctgctg gcggcctggt
     9841 gctttgcccg ttcttccccg cgctttcacg cctggttgct gtaccgctca tggtttggca
     9901 gctatctacg tttctggcag aaacatcatg cgatgccgcg cggcgtcaaa ccgcgggcga
     9961 ttttgcttat tttgctcacg tttgccattt ctctgtggtt cgtccagatg ccatgggtgc
    10021 gcatcatgtt gctggtaatt ctcgcctgtt tgcttttcta tatgtggcga attccggtga
    10081 ttgatgaaaa gcaagaaaag cactgaagca caacaatcgc agttgcaatt attgcgtaca
    10141 gccagtacat tctggcgttt tcgagcacag gcgcaggcgg tcaaaggtta aacaactgtt
    10201 acttttgata cgtttaaaac gcgccgtgag taccaccgta acaagcaggc atacacttat
    10261 gaccgcgact gcacagcagc ttgagtatct caaaaatagc atcaaaagca ttcaggacta
    10321 cccaaaaccc ggcattcttt tccgcgatgt caccagctta ctggaagacc cgaaagctta
    10381 cgctctcagc atcgacttgc tggttgagcg ttacaaaaat gcgggcatta ccaaagttgt
    10441 cggcaccgaa gcgcgtggct tcttgtttgg cgctccggta gctctgggtc tgggcgttgg
    10501 ctttgtaccg gtccgtaaac cgggcaaact gccgcgtgaa actatcagtg aaacttacga
    10561 tctggaatac ggcaccgatc agctggagat ccacgttgat gccatcaaac cgggcgacaa
    10621 agttctggtg gtggacgatc tgctggcaac cggcggcact atcgaagcga ccgttaaact
    10681 gatccgtcgt ctgggtggtg aagtggctga cgctgcgttc attatcaacc tgttcgatct
    10741 cggcggcgaa cagcgtctcg aaaaacaggg cattaccagc tacagccttg tcccgttccc
    10801 gggccattaa ttatcgccag tcttgtgctg cccacgctac ggacagcaca agatgtgcat
    10861 tcagcctcgc cgttctgacg gggctgtgtt agcattaccc cttcgtgaat ccaccttcca
    10921 gcgtttcaga gcctgccaat gagttatcag gtcttagccc gaaaatggcg cccacaaacc
    10981 tttgctgacg tcgtcggcca ggaacatgtg ctgaccgcac tggcgaacgg cttgtcgtta
    11041 gggcgtattc atcatgctta tcttttttcc ggtacccgtg gcgtcggaaa aacctctatc
    11101 gcccgactgc tggcgaaggg gctaaactgc gaaaccggca ttaccgcgac gccgtgcggt
    11161 gtgtgcgata actgtcgtga aatcgagcag gggcgctttg tcgatctgat tgaaatcgac
    11221 gccgcctcgc gcaccaaagt tgaagacact cgcgacctgc tggataacgt ccagtacgct
    11281 ccggcgcgtg gtcgtttcaa agtttatctc atcgacgaag tgcatatgct gtcgcgtcac
    11341 agctttaacg cactgttaaa aactcttgaa gagccgccgg agcacgttaa gtttctgctg
    11401 gcgacgaccg atccacagaa attgccggtg acgattttgt cgcgctgttt gcaatttcat
    11461 ctcaaggcgc tggatgtcga gcaaattcgc catcagcttg agcacatcct caacgaagaa
    11521 catatcgctc acgagccgcg ggcgctgcaa ttgctggcac gcgccgctga aggcagcctg
    11581 cgagatgcct taagtctgac cgaccaggcg attgccagcg gtgacggtca ggtttcaacc
    11641 caggcggtca gtgcgatgct gggtacgctt gacgacgatc aggcgctgtc gctggttgaa
    11701 gcgatggtcg aggccaacgg cgagcgcgta atggcgctga ttaatgaagc cgctgcccgt
    11761 ggtatcgagt gggaagcgtt gctggtggaa atgctcggcc tgttgcatcg tattgcgatg
    11821 gtacaacttt cgcctgctgc acttggcaac gacatggccg ccatcgagct gcggatgcgt
    11881 gaactggcgc gtaccatacc gccgacggat attcagcttt actatcagac gctgttgatt
    11941 ggtcgcaaag aattaccgta tgcgccggac cgccgtatgg gcgttgagat gacgctgctg
    12001 cgcgcgctgg cgttccatcc gcgtatgccg ctgcctgagc cagaagtgcc acgccagtcc
    12061 tttgcacccg tcgcgccaac ggcagtaatg acgccaaccc aggtgccgcc gcaaccgcaa
    12121 tcagcgccgc agcaggcacc gactgtaccg ctcccggaaa ccaccagcca ggtgctggcg
    12181 gcgcgccagc agttgcagcg cgtgcaggga gcaaccaaag caaaaaagag tgaaccggca
    12241 gccgctaccc gcgcgcggcc ggtgaataac gctgcgctgg aaaggctggc ttcggtcaca
    12301 gatcgcgttc agacgcgtcc ggtgccatcg gcgctggaaa aagcgccagc taaaaaagaa
    12361 gcgtatcgct ggaaggcgac cactccagtg atgcagcaaa aagaagtggt cgccacgccg
    12421 aaggcgctga aaaaagcgct ggaacatgaa aaaacgccgg aactggcggc gaagctggcg
    12481 gcagaagcca ttgagcgcga cgcgtgggcg gctcaggtga gtcaactttc gctaccaaaa
    12541 ctggtcgaac aggtggcgtt aaatgcctgg aaagaggaga gtgataacgc agtatgtttg
    12601 catttgcgct cctctcagcg gcatttgaac aaccgcggtg cacagcaaaa actggctgaa
    12661 gcgttgagca tgttaaaagg ttcaacggtt gaactgacta tcgttgaaga tgataatccc
    12721 gcggtgcgta cgccgctgga gtggcgtcag gcgatatacg aagaaaaact tgcgcaggcg
    12781 cgcgagtcca ttattgcgga taataatatt cagaccctgc gtcgtttttt cgatgcggag
    12841 ctggatgaag aaagtatccg ccccatttga tcgtaagcac agcttacgtt cgtcatcctt
    12901 aacgtgattg agagagaaac ctatgtttgg taaaggcggt ctgggtaacc tgatgaagca
    12961 agcccagcag atgcaagaaa aaatgcagaa aatgcaggaa gagatcgcgc agctggaagt
    13021 caccggcgaa tctggcgcag gtctggtaaa agtgaccatc aacggtgcac acaactgccg
    13081 tcgcgtagag atcgacccga gcctgctgga agacgacaaa gagatgctgg aagacctggt
    13141 ggctgcagca ttcaacgacg cagcacgtcg tattgaagaa acgcagaaag aaaaaatggc
    13201 ctctgtatcc tccggaatgc aactgccgcc tggctttaag atgccgttct gatgcaaacc
    13261 agcccgctgt taacacagct tatggaagca ctgcgctgtc tgccgggcgt tggcccgaag
    13321 tcggcgcagc gtatggcgtt cacgctgctt cagcgcgatc gtagcggcgg gatgcgtctg
    13381 gcgcaggcgc tcacccgggc gatgtcggaa atcggccact gcgccgattg ccgcactttc
    13441 accgaacagg aagtctgtaa catctgttcg aatccgcgtc gtcaggaaaa cggtcaaatc
    13501 tgtgtggtgg agagtccggc ggacatttac gccattgagc agacggggca gttttcaggt
    13561 cgttattttg tgttgatggg gcatctgtca ccgctggacg gcatcggtcc ggatgatatc
    13621 gggcttgatc gtctggaaca gcgtctggca gaggaaaaaa tcactgaagt gatcctcgcc
    13681 accaatccca cggttgaagg tgaagctacc gctaactaca ttgccgagct ttgcgcgcaa
    13741 tatgacgtgg aagccagccg aatcgctcat ggcgtaccgg ttggcggcga gctggaaatg
    13801 gtcgacggca ccacgttgtc acactccctt gccgggcgtc ataagattcg tttttaagca
    13861 aacgagagca ggatcacctg ctctcgcttg aaattattct cccttgtccc catctctccc
    13921 acatcctgtt tttaacctta aaatggcatt attgaggtag acctacatga aaggacaaga
    13981 aactcgtggt tttcagtcag aagtgaaaca gcttctgcac ctgatgatcc attctctcta
    14041 ttccaataaa gaaatcttcc tgcgtgagct tatctctaac gcctccgatg cggcggacaa
    14101 gctgcgtttc cgtgcgctct ctaacccgga cctgtacgaa ggtgatggcg aactacgcgt
    14161 tcgtgtctct ttcgataaag acaagcgtac gctgaccatc tccgataacg gcgtggggat
    14221 gacccgcgac gaagtgattg accatctggg gactatcgct aaatccggta ccaaatcatt
    14281 cctcgaatcc ctgggttctg accaggcgaa agacagccag ctgatcggtc agtttggtgt
    14341 tggtttctac tctgcgttta tcgtggccga caaagtgacc gtgcgtactc gcgcggcagg
    14401 cgaaaaacca gaaaatggcg tcttctggga atcggctggc gaaggtgaat acaccattgc
    14461 cgacatcacc aaagaagatc gtggtactga aatcaccctg catctgcgtg aaggcgaaga
    14521 cgagttcctc gatgactggc gcgtgcgttc catcatcagc aaatactccg accatatcgc
    14581 gctgccggta gagatcgaaa aacgcgaaga gaaagacggc gaaaccgtta tctcctggga
    14641 gaaaatcaac aaagcgcagg cgctgtggac tcgtaacaag tcggaaatca ccgatgaaga
    14701 gtacaaagag ttctacaaac acatcgccca cgactttaat gatccgctga cctggagcca
    14761 caaccgtgtt gaaggtaagc aggagtacac cagcctgctg tacatcccgt cccaggctcc
    14821 gtgggatatg tggaaccgcg atcataaaca cggcctgaaa ctgtatgttc agcgtgtgtt
    14881 catcatggac gacgcagaac agttcatgcc gaactatctg cgcttcgtgc gtggtctgat
    14941 tgacgccagc gatctgccgc tgaacgtttc ccgtgaaatc ctccaggaca gcacggtaac
    15001 gcgtaacctg cgcaatgcgc tgaccaagcg tgtgctgcaa atgctggaaa aactggcgaa
    15061 agacgacgcg gaaaaatacc agaccttctg gcaacagttt ggcctggtac tgaaagaagg
    15121 tccggcggaa gatttcgcta accaggaagc gatcgccaaa ctgctgcgtt ttgcttctac
    15181 ccataccgat tcttctgcgc agaccgtatc tctggaagac tacgtttccc gcatgaaaga
    15241 agggcaggag aaaatctact acatcaccgc agacagctat gcggcagcga agagcagccc
    15301 gcacctggaa ctgctgcgta agaaaggcat cgaagttctg ctgctttccg accgcatcga
    15361 tgagtggatg atgaactatc tgactgagtt cgacggtaaa ccgttccagt cggtgtctaa
    15421 agttgacgag tcgcttgaaa aactggctga cgaagttgat gagagcgcga aagaagcgga
    15481 gaaagcactg actccgttca tcgaccgtgt gaaagccctg ctcggcgagc gcgtgaaaga
    15541 tgtccgtctg actcaccgtc tgaccgatac gccagcgatc gtttcgaccg acgcggacga
    15601 aatgagcact cagatggcga aactgttcgc tgcggcgggc cagaaagtgc cagaagtgaa
    15661 atacatcttc gaactgaacc cggatcacgt actggtgaaa cgtgcggcag atactgaaga
    15721 tgaagcgaag ttcagcgagt gggtagaact gctgctggat caggcgctgc tggcagaacg
    15781 cggcacgctg gaagatccga acctgtttat tcgtcgtatg aaccagctgc tggtttcctg
    15841 atgtaatgcc ggatgacctt cgtgtcatcc ggcatttttc ttttcatcat ctgcactttc
    15901 cgcaaattat ctcgccatta accgtttcag ccccaggtgc ctttcttgag gcaatcgcct
    15961 gttggtggta tcgtttatcg ctttttcaaa aaattcgaca cattttaagg ggattttcgc
    16021 aatgcgtatc attctgcttg gcgctccggg cgcggggaaa gggactcagg ctcagttcat
    16081 catggagaaa tatggtattc cgcaaatctc cactggcgat atgctgcgtg ctgcggtcaa
    16141 atctggctcc gagctgggta aacaagcaaa agacattatg gatgctggca aactggtcac
    16201 cgacgaactg gtgatcgcgc tggttaaaga gcgcattgct caggaagact gccgtaatgg
    16261 tttcctgttg gacggcttcc cgcgtaccat tccgcaggca gacgcgatga aagaagcggg
    16321 catcaatgtt gattacgttc tggaattcga cgtaccggac gaactgatcg ttaaccgtat
    16381 cgtcggtcgc cgcgttcatg cgccgtctgg tcgtgtttat cacgttaaat tcaatccgcc
    16441 gaaagtagaa ggcaaagacg acgttaccgg tgaagaactg actacccgta aagatgatca
    16501 ggaagagacc gtacgtaaac gtctggttga ataccatcag atgacagcac cgctgatcgg
    16561 ctactactcc aaagaagcag aagcgggtaa taccaaatac gcgaaagttg acggcaccaa
    16621 gccggttgct gaagttcgcg ctgatctgga aaaaatcctc ggctaattcg aaagcgcgca
    16681 cggacagtcc cctcgccccc tcggggagag ggttagggtg aggggaacag gcccgcacaa
    16741 gcaaacttat cagcaatctc aggccggata ttcattcggc cttttacaaa aacctcaatc
    16801 tgcgctattc ctttttctga tttgacctct cacagcaatt agttcttctt cctcactttt
    16861 ccgctacaat tatcaacaag ttgaatcgat aagaggcggt aatgcgtcag actaaaaccg
    16921 gtatcctgct ggcaaacctg ggtacgcccg atgcccccac acctgaagcg gtaaaacgct
    16981 atctgaaaca atttttaagc gacagacgcg tggttgatac ctcacggttg ttatggtggc
    17041 cattgctgcg cggcgtgatt ttgccgctgc gctcgccgcg tgtggcgaag ctgtatgcct
    17101 ctgtctggat ggaaggtggc tcgccgctga tggtttacag ccgccagcaa cagcaggcgc
    17161 tggcacaacg tttaccggag atgcccgtag cgctgggaat gagctacggc tcgccatcac
    17221 tggaaagcgc cgtagatgaa ctcctggcag agcatgtaga tcatattgtg gtgctgccgc
    17281 tttatccgca attctcctgt tctacggtcg gtgcggtatg ggatgaactg gcacgcattc
    17341 tggcgcgcaa acgtagcatt ccggggatat cgtttatacg tgattacgcc gataaccacg
    17401 attacattaa tgcactggcg aacagcgtac gcgcttcttt tgccaaacat ggcgaaccgg
    17461 atctgctact gctctcttat catggcattc cccagcgtta tgcagatgaa ggcgatgatt
    17521 acccgcaacg ttgccgcaca acgactcgtg aactggcttc cgcattgggg atggcaccgg
    17581 aaaaagtgat gatgaccttt cagtcgcgct ttggtcggga accctggctg atgccttata
    17641 ccgacgaaac gctgaaaatg ctcggagaaa aaggcgtagg tcatattcag gtgatgtgcc
    17701 cgggctttgc tgcggattgt ctggagacgc tggaagagat tgccgagcaa aaccgtgagg
    17761 tcttcctcgg tgccggcggg aaaaaatatg aatatattcc ggcgcttaat gccacgccgg
    17821 aacatatcga aatgatggct aatcttgttg ccgcgtatcg ctaaagctga gcggtaaaga
    17881 actgagcgcc gtcgcgaaga gcctcgtcgg cggttttcat catccgtgaa taatgcaaaa
    17941 aggcgtgcag cgtgcctggg tagagtttga actcacaggg ctgctgatgc gccgctaacg
    18001 tctggtaaag cagacggctg tcatccagca gcggatcgaa ctccgccccg gcaataaaac
    18061 agggcggaac ttcgcgagtg agatcattat taaacagaca gtaatacggc gactcgcggt
    18121 ccgcgtcgtt gcttaaatat gcctcttcgt acatctgcaa atcctgttgc gttaagccat
    18181 cccagacacc gcccaacaga cgacgagtca cggaatcccg taatccgtaa agcccatacc
    18241 acagcaaaac gcccgcaact ttaccgcaat cgatctgttt atcacgcaac cacaacgcac
    18301 tggcgagcgc cagcatggca cctgcggaat caccggcaaa gccaatgcgg gacatattga
    18361 tttgataatc ctccgcctgc tggtggaaat aacaacaagc agccacaatt tcctctatcg
    18421 cttgcggaaa acgcgcttca ggtgaaaggg tgtaatcaat accaatcacc gtacattggc
    18481 tgtagcttgc cagcaggcgc atgatgcgat cgtgggtatc gagattgccg agaataaaac
    18541 cgcctccatg caaataaaat agcgtcgctg ggctatctgg ctgcggacaa aagagacgtg
    18601 tttccacctg cccatatttt gttggaacca tgtaagctct ggttgccatt tctggagcgc
    18661 ccgcattcca gaatcggcgc tcaagcgtgt aatactgtcg ttgctcagca atcgttcccg
    18721 ttgcgggcca gggcggtaaa tccggctgaa gagtattcac aacggtcttc atttcagcag
    18781 aaataaggtc cagaacgggt aatttgtttt ccggcttcat acaaaaactc ctttcaaatt
    18841 acgtcattgt aaggaaccac tgccatgaaa atgcgatccc gcctgctgat attgaaactg
    18901 gctgcgtctc gcgcgctccc gtcagattgt gttaacattc gccgctcagt taaccacccg
    18961 taaaaacaac catgaaattt cccggtaaac gtaaatccaa acattacttc cctgtaaatg
    19021 cacgcgatcc gctgcttcag caattccagc cagaaaacga aaccagcgcc gcctgggtag
    19081 tgggtatcga tcaaacgctg gtcgatattg aagcgaaagt ggatgatgaa ttcattgagc
    19141 gttatggatt aagcgccggg cattcactgg tgattgagga tgacgtagcc gaagcgcttt
    19201 atcaggaact aaaacagaaa aacctgatta cccatcagtt tgcgggtggc accattggta
    19261 acaccatgca caactactcg gtgctcgcgg acgaccgttc ggtgctgctg ggcgtcatgt
    19321 gcagcaatat tgaaattggc agttatgcct atcgttacct gtgtaacact tccagccgta
    19381 ccgatcttaa ctatctacaa ggcgtggatg gcccgattgg tcgttgcttt acgctgattg
    19441 gcgagtccgg ggaacgtacc tttgctatca gccctggcca catgaaccag ctgcgggctg
    19501 aaagtattcc ggaagatgtg attgccggag cctcggctct ggttctcacc tcttatctgg
    19561 tgcgttgcaa gccgggtgaa cccatgccgg aagcaaccat gaaagccatt gagtacgcga
    19621 agaaatataa cgtaccggtg gtgctgacgc tgggcaccaa gtttgtcatt gccgagaatc
    19681 cgcagtggtg gcagcaattc ctcaaagacc acgtctctat ccttgcgatg aacgaagatg
    19741 aagccgaagc gttgaccgga gaaagcgatc cgttgttggc atctgacaag gcgctggact
    19801 gggtagatct ggtgctgtgc accgccgggc caatcggctt gtatatggcg ggctttaccg
    19861 aagacgaagc gaaacgtaaa acccagcatc cgctgctgcc gggcgctata gcggaattca
    19921 accagtatga gtttagccgc gccatgcgcc acaaggattg ccagaatccg ctgcgtgtat
    19981 attcgcacat tgcgccgtac atgggcgggc cggaaaaaat catgaacact aacggagcgg
    20041 gggatggcgc actggcagcg ttgctgcatg acattaccgc caacagctac caccgtagca
    20101 acgtaccaaa ctccagcaaa cataaattca cctggttaac ttattcatcg ttagcccagg
    20161 tgtgtaaata tgctaaccgt gtgagctatc aggtactgaa ccagcattca cctcgtttaa
    20221 cgcgcggctt gccggagcgt gaagacagcc tggaagagtc ttactgggat cgttaagtta
    20281 tcgtcggttc gtaggccaga taaggcgttc acgccgcatc tggcatttgg ctctcgatgc
    20341 ctgatgcgac gctggcgcgt cttatcatgc ctacatattt ttcatatttt acatccggca
    20401 accaccgttt accccgtcac cacctcaccc gccggtggcg tttccagcag ttccagcatg
    20461 gtacgggcga tttcacgctc gcccatcact acctgattcg caccacgttc ggtgatatac
    20521 gccacttcat cgtcataatg ggcgcgggca ataatctcaa tatccggatt tttcgcgcgg
    20581 gcagatgcca caatctcacc cgcttcataa ccgttgggaa tcgtcaggat cagccatttt
    20641 gcacattcca gatgcgccag ttgcataatt tcttcgttcg ccgcattgcc caatactgcg
    20701 cggaccccgc gctctcgcag ctcatcaaca cgggttcgtg acgtctcaat caccaccagc
    20761 ggaatatcag aggcgagcaa tttctccccc agcaggctgc ctacacgacc gtaacccacc
    20821 agtagcgcat ggttgcaaat atccactggg atctgcttct cttcttcgat tgcctcttcc
    20881 agcgtctgct cttccagcgt ttcggtcttc gccagatatt tctccagtag tgcgaacagt
    20941 accgggttga gcataatcga caggatcgcc cctgccagta ccaggttttg tccggcctgc
    21001 ggcagtaaat tcaatgccat tcccagtccc gccaggataa acgcgaactc accaatctgc
    21061 gccaggctgg cggcgatggt taatgccgta cgttgggagt gaccaaacag tcgcaccagg
    21121 aaaaatgcgg ctaacgactt accaaacaga ataatcgcca gcgtcgccag cactgccagc
    21181 ggttgctgaa tcagaattaa cggatcaaac aacatcccga cggagacaaa aaacagcacc
    21241 gcaaacgcgt cgcgcaatgg cagcgtatcg tgggcggcac ggtgactcag ttcagactcg
    21301 ttcagtacca tcccggcaaa gaacgcaccg agtgcaaagg agacatcaaa cagctctacc
    21361 gcaccaaagg caacccctaa cgccagcgcc agcaccgaca gggtaaacag ctcgcgagaa
    21421 ccggttgccg cgctgcgtgc cataatccac ggcaccagac ggcgacctac cagcatcata
    21481 atggcgataa atgcgatcac tttgccgatg gtgatcccca tatcgactgc aagagtggca
    21541 aagcccacat cgccctgttc catcattcct gccactgcgg gcagcaacac cagcgtcaga
    21601 accattacca ggtcttccac aatcaaccaa ccgatggcga tttgcccacg ctgactgtca
    21661 attaattgcc gttcttcaag tgcgcgcagt aacaccacgg tactggcggt ggaaagacat
    21721 aaaccgaaca cgataccggt cattaacgac cagcccagca cggcagagag cgccataccc
    21781 agcagcgtcg ccacggctat ctgggcgatc gcaccgggaa tggcgatggc ctttaccgcc
    21841 atcaaatcct tcagcgaaaa gtgcaaaccg acgccaaaca tcaacagaat gacgcccagt
    21901 tcagccagtt ccggggcaag cttggtatcg gcaacaaagc ccggagtgaa tggtcctgcc
    21961 agcacacccg ctaacagata tcccaccaga ggagaaatac gtagtttatt ggccagcatg
    22021 ccgaggataa aggcgagcac aaggccgcca acaatggtgg tgataagcgg ggtggcgtga
    22081 tgcattccgt ctccttttcc tggtggttat tgtccatttt tggccgggaa aaccaaaatt
    22141 acaggtaata gtttatgaca atttcattga tgatgtttat gaataattgt tgaattttgc
    22201 agaaaaatgg aattagctgc aaaaaaagca cggatcggaa aacagaaggc ggtttaacag
    22261 aggaaaggct tatggcgact gtaaaacgat gcagccaaag tttgcctttg gctgcaatga
    22321 aatcagtctt tatgccggtt atcaggcagg aatatggtca acatccccaa tagtggcagg
    22381 aaagcacaga ttttatagac taactcgatg ctggtgtgat cggcgataag ccccagaact
    22441 gccgcaccca gacctcccat gccgaaagca aaaccgaaaa agagtccaga aaccataccg
    22501 atacgtcccg gaagcagctc ctgagcgtaa accagaatgg cagagaatgc cgaagcgagg
    22561 ataaatccaa taatcaccgt taaaaccccc gtccagtgca ggctggcgta gggtaaaatc
    22621 agcgtaaacg gcgcaacgcc gaggatagag ccccaaatca catatttccg cccaatttta
    22681 tcccctacag gcccgccgat caccgtacct gccgcaacgg caaacaggaa ggcaaacaga
    22741 tgaagctgag cattctggat agataatccg aatttttgca tcagatagaa ggtgtaatag
    22801 ctgctgatgc tcgccatata gaaatatttc gagaaaatga ggattaacag aatgctgacc
    22861 gccagtacca ctttgttgcg cggcagtgga ttgataatcg tcgctttggg ttttccttta
    22921 ttcattcggt gctgtgccga gtaccaacgg ctgatttgtg ccaacaccac gatcgccagc
    22981 agtgccgcaa gcacaaacca ggcaacgttg cctttgccat aaggcgcgat aatcaccgcc
    23041 gccagcaagg gtcccaggga actgccaaag ttgccgccga cctgaaagat agattgtgcc
    23101 aggccatgcc gcccgccgga agccatacgg gccacgcgag aagattccgg atgaaagacc
    23161 gatgaaccgg taccgaccag cgccgccgcc agcagaactg cgccaaaact gcccgccagc
    23221 gcaagcagca ctagaccact taaggtaaag cacatgccaa ttggcaacga ccatggcatt
    23281 ggatatttat cggtccagta gccgaccact ggttgcagta gcgaagaggc gagctggaag
    23341 gtgagggtta tcatgccaat ctgcataaat gtcagagaaa attctgactg aagcagcgga
    23401 taaatcgcca gaatcagcga ttggatcatg tcgttcagca gatgtgagag gctgatagca
    23461 cctaaaatac caaacgatgt tcgggccttg gtcgttgacg cagccgcgcc cgccacaggc
    23521 tgggtttgtt cactcattgc cataggaaag tcactttttc agggttgcga tgtaaagaat
    23581 gatcttattt gtgattatta cccgactaac atacctgtat gcgtcgcctg aaggaagtct
    23641 caacgccgaa tacagaattt ctaatctgga tgcagattta tcttcaccgg acgcagactt
    23701 gtctatgatg tcgcgtcata ctatttttca acacgttgaa atcaggtcag ggagagaagt
    23761 atgaaattat tgcagcgggg cgtggcgtta gcactgttaa ccacatttac actggcgagt
    23821 gaaactgctc tggcgtatga gcaggataaa acctacaaaa ttacagttct gcataccaat
    23881 gatcatcatg ggcatttttg gcgcaatgaa tatggcgaat atggtctggc ggcgcaaaaa
    23941 acgctggtgg atggtatccg caaagaggtt gcggctgaag gcggtagcgt gctgctactt
    24001 tccggtggcg acattaacac tggcgtgccc gagtctgact tacaggatgc cgaacctgat
    24061 tttcgcggta tgaatctggt gggctatgac gcgatggcga tcggtaatca tgaatttgat
    24121 aacccgctca ccgtattacg ccagcaggaa aagtgggcca agttcccgtt actttctgcc
    24181 aatatctacc agaaaagtac cggcgagcgc ctgtttaaac catgggcgct gtttaagcgt
    24241 caggatctga aaattgccgt tattggtctg acgacggatg acacagcgaa gttaggcaat
    24301 ccagaaaact tcaccgatat tgagttccgt aagcccgccg atgaagcgaa gctggtgatt
    24361 caggagctgc aacagacaga aaagccagac attattatcg cggcgaccca tatggggcat
    24421 tacgacaatg gtgagcacgg ctctaacgca ccgggcgatg tggagatggc gcgcgcgctg
    24481 cctgccggat cgctggcgat gatcgtcggt ggtcactcgc aagatccggt ctgcatggcg
    24541 gcagaaaaca aaaaacaggt cgattacgtg ccgggtacgc catgcaaacc agatcaacaa
    24601 aacggcatct ggattgtgca ggcgcatgag tggggcaaat acgtgggacg ggctgatttt
    24661 gagtttcgta atggcgaaat gaaaatggtt aactaccagc tgattccggt gaacctgaag
    24721 aagaaagtga cctgggaaga cgggaaaagc gagcgcgtgc tgtacacccc tgaaatcgct
    24781 gaaaaccagc aaatgatctc gctgttatcg ccgttccaga acaaaggcaa agcgcagctg
    24841 gacgtgaaaa taggcgaaac caatggtcgt ctggaaggcg atcgtgacaa agtgcgtttt
    24901 gtacagacca atatggggcg gttaattctg gcagcccaaa tggatcgcac tggtgccgac
    24961 tttgcggtga tgagcggagg cggaattcgt gattctatcg aagcaggcga tatcagctat
    25021 aaaaacgtgc tgaaagtgca gccattcggc aatgtggtgg tgtatgccga catgaccggt
    25081 aaagaggtga ttgattacct gaccgccgtc gcgcagatga agccagattc aggtgcctac
    25141 ccgcaatttg ccaacgttag ctttgtggcg aaagacggca aactgaacga ccttaaaatc
    25201 aaaggcgaac cggtcgatcc ggcgaaaact taccgtatgg cgacattaaa cttcaatgcc
    25261 accggcggtg atggatatcc gcgccttgat aacaaaccgg gctatgtgaa taccggcttt
    25321 attgatgccg aagtgctgaa agcgtatatc cagaaaagct cgccgctgga tgtgagtgtt
    25381 tatgaaccga aaggtgaggt gagctggcag taatccgaaa gtgccggatg tttgcatccg
    25441 gcacaatgct taatcgcggc gggcgatatc agcaaatttg gcatcgagga tctttgccag
    25501 atcgcctgcc gccagttcga tatccagtcc gcgcttgccg ccggaaacat aaatagtggc
    25561 aaattcttgt gcgggggcgt cgataatcgt tggcagacgt tttttctgcc ccagtgggct
    25621 aatcccccca accaggtatc ccgtcgaacg ctgcgcgacc atcggatcgg ccatctcaac
    25681 tttcttggca cccagcgctt ttgctacttt tttaagatcc agttgacctg cgaccggcgt
    25741 aacggccacg gcaaggtgtt tcatatcacc gttcactgcc accagcagcg ttttgtagac
    25801 ctgatccgga ttcaaaccta attttttgac gacttcatcg ccaaaattgg tttcagccgg
    25861 atcgtgctcg taggtatgga tttgaaacga aatcttgttt ttttcgagta atttaactgc
    25921 gggtgtcatg gtaattttcc tgccttaaac aaaaaaacga agcaagcata cgcctgatgg
    25981 cggtccaaaa aaaagagtca tcttgcctaa gagtattggc aggatggtga gattgagcga
    26041 caatcgagtt acaccgtcgc ttaaagtgac ggcataataa taaaaaaatg aaattcctct
    26101 ttgacgggcc aatagcgata ttggccattt ttttagcgca acatttgcgg taaattccct
    26161 tctccataca ggtgtagtgc accgaccgcg accacatatc gccccggcgg catggcgcgt
    26221 aatttatccc gccaggcgag atttcgctga tgcatcagca catcgtacag cgactgactg
    26281 aacgtattgg gcagcgttat atcattattt tgcggcggtg cattcagcca ccagctcatc
    26341 atttgttgca gcaaccgtgc gttggtatgc cagtgggtca gcgtatcgtc cagcagcgcc
    26401 agtcctttgt cagggagctg gagcaacatg gcaatctggt tttcagcccc ttccagttca
    26461 atcacgggtt tatgttgttg cttcgccgcc tgcaatagct ggtaatcgat accgtattct
    26521 gcccgcagcc ccagtttttg tgcctgcgtc gcctgaagaa ccatcgcgat ttgccacagc
    26581 ggttgggtag aaaagagtga gggagaaatg cccatctcct ggctaatgtg ctgcaggttt
    26641 tggagttgct cctcgctaat gcgctcttcc agcgcctcgc aggcaggcaa attagcaaaa
    26701 ggcgtatcgc tggtggaaac atccgcctcg acgatcagcg catcggcgtt tttgagcttt
    26761 ttgagcaaac gggtgggcag gggagccata tcgtggctac ccatatgaat gctgccaatc
    26821 agatgaaaat ggcgattgcc ggggagggtg atatcgatgg caggccagga gtaatgatta
    26881 ccgcgcagcg cggcccaaag cgtttttacc cggtacaaca gatccatacg acctcccttt
    26941 gtgaaatatc atgctagcgc gcggggaggg atggcgcaaa tggagttggg gacttgttta
    27001 atgtgtttgt atgattcagt atgttcttgt atcgctattc acagggaagc aacagttaaa
    27061 aactatgaaa caggcaacaa gaaaaccgac gacacccgga gatattcttc tctatgaata
    27121 tctggaaccg ctcgatttga aaatcaatga gttagcagag ttgctgcatg ttcatcgtaa
    27181 tagcgtcagt gcactgatca ataacaatcg taaactcacc actgagatgg cattccgtct
    27241 ggcgaaagtt tttgatacca cagtcgattt ttggctaaac ctccaggcgg cagttgatct
    27301 ttgggaagtt gaaaacaaca tgcgcaccca ggaagaattg ggacggattg aaacagtggc
    27361 tgaatatttg gcacgccgtg aagagcgtgc aaaaaaggtc gcgtaaggcc aaaacatcgc
    27421 ttcatctcgt aggccggata aggcgttcac gccgcatccg gcatccgagc accattgcct
    27481 gatgcgacgc ttacccgtct tatcaggcct acaaacttgt gcctgaaccg taggccggat
    27541 aaggcgttta cgccgcatcc ggcatccgag caccattgcc tgatgcgacg cttacgcgtc
    27601 ttatcaggcc tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc
    27661 cggcagtcat gcgtcgatgc caaatgcgcc accctaaagc agcgcatccg caatgatgta
    27721 cttattcctt cggtttaaac cgcagcaacc ggttggcgtt actcactacg gtaatcgacg
    27781 agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagtg aacggccaca
    27841 aaataccggc ggcgaccgga ataccgatac tgttgtagat aaacgcaccg agcaggttct
    27901 gcttcatgtt gtgcagcgtt gcgcgggaaa tagcgagcgc atccgcaacg cccatcaggc
    27961 tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg ccacccatcg
    28021 caatgccgac atccgcctga gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg
    28081 ccacctgacg tccttcactt tgcagatgtt tgatcgcttc ggctttaccg tccggcagca
    28141 ccccggcgat cacctcatca atccctgctt ctttggcgat cgcattggcg gtggttgggt
    28201 tatccccggt caacatcacc agacgatatc ccgctttatg caggcgttgc agcgccgcca
    28261 cgctatcact acgcaacgga tcgcgtactg ccagcagggc taccgctttc ccgtcaaccg
    28321 ccagcagcac aggcgttgcc ccttgcgatg cctgagcagt aatctccgct tcgatagctt
    28381 tggtaccaac ctgttgctca tttaacagcg cctgattgcc cagcaataac gcatgacctt
    28441 cagcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggtagct
    28501 gcatatcacc tgctttatcg aggatcgccc gtgccagcgg atggctggaa ccttgctcca
    28561 gtgccgccgc cagacgcaat gcctgcgctt catcaacatc agcaaatgtt ttcactgcga
    28621 caacctgcgg cttcccttca gtcagcgtcc cggttttatc gaacactaca gtgtcgagtg
    28681 tactggcgcg ttgcagcgcg tcagcgtccc gcaccagcac gccaaactca gccgcccgcc
    28741 cgacgccgga aataatcgac atcggcgtcg ccagccccag cgcacacgga caggcaataa
    28801 tcagtaccgt ggtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat
    28861 accagattgc cgcactgaca agcgcaataa ccaccactac cggcacaaat acggctgaga
    28921 ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc accatgcgaa
    28981 tgattcgtga cagcgtagta tggctgccaa ccgcactggc acgaaacagc acactgccgt
    29041 cctgtaccac tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggattggtt
    29101 cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctaccg
    29161 gcacgcgatc gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca
    29221 cgcttttttc accttcgtca gtaaccaggc gtgccgtcgg cggggttaaa tcgagtaact
    29281 tttccagcgc cttagaagaa cgctggcgtg cgcgcgcttc cagcatatgg ccgagattga
    29341 tcagaccgat aatcatcgcg ctggcttcgt aataaagatg tcgcgcttcc atcgggaacc
    29401 actgcggcca caggttgacg ctcatcgaat agagccacgc cacgccagta cccagcgcca
    29461 ccagcgtatc catcgtcgcc gcaccgttca gcaggctttt ccatgcactg cggtaaaaat
    29521 ggccgccggc gaaaaccatc actgccaggg ttatcaggcc gataaccaac cacaggctgc
    29581 ggttgtcagc ggtgaccatc atgttatcgc cgatcatccc ccagaccatc accgggatac
    29641 ccaccgccag tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt
    29701 cttgctggcg ctcgcggcgt ttagcgtcat cttcaatcgc ttccgcgccg tagcccgctt
    29761 tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct
    29821 ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc
    29881 gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat
    29941 cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg
    30001 actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga
    30061 tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca
    30121 catccgcctg ctcaacatcc ggacgctgtt caagactttc tttcacgcgt ttaacgcagt
    30181 gaccgcagga caggccgtcc agggtcaggt cgatagtttg tgacataaaa cactccttta
    30241 agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa
    30301 ttaataaacc aggcgggtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag
    30361 gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag
    30421 gatgcctgaa aatcggcacc ggggtgagga attacctccc gcatctataa aaaggagtta
    30481 acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt
    30541 tcactcactt aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg
    30601 tcaactggcg gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag
    30661 tgattaccgc tttgcactgg aatccatctc gaaagtctgt acgttagccc ttgcgttaga
    30721 agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt
    30781 taactcagtt atcgccttag agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc
    30841 tggcgctatt gccaccacca gcctgattaa cgctgaaaat gttgaacaac gctggcagcg
    30901 aattttacat atccaacagc aactggctgg cgagcaggta gcgctctctg acgaagtcaa
    30961 ccagtcggaa caaacaacca acttccataa ccgggccata gcctggctgc tgtactccgc
    31021 cggatatctc tattgtgatg caatggaagc ctgtgacgtg tatacccgtc agtgctccac
    31081 gctcctcaat actattgaac tggcaacgct tggcgcgacg ctggcggcag gtggtgtgaa
    31141 tccgttgacg cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat
    31201 gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgtgttg gtttaccggg
    31261 caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggagtgatgg gaattgccgc
    31321 gttctcacca ccgctggacg aagatggcaa cagtgttcgc ggtcaaaaaa tggtggcatc
    31381 ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt
    31441 aataacggta acaaacctct cggtctatgg aacgtcgttt cc
//
LOCUS       DAZFZC010000055        31461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFZC010000055 DAZFZC010000000
VERSION     DAZFZC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.055"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..567
                     /locus_tag="ACHKXP_002858"
     CDS             <1..567
                     /locus_tag="ACHKXP_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO7647830.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            797..1180
                     /gene="secE"
                     /locus_tag="ACHKXP_002859"
     CDS             797..1180
                     /gene="secE"
                     /locus_tag="ACHKXP_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO7647831.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1182..1727
                     /gene="nusG"
                     /locus_tag="ACHKXP_002860"
     CDS             1182..1727
                     /gene="nusG"
                     /locus_tag="ACHKXP_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO7647832.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1886..2314
                     /gene="rplK"
                     /locus_tag="ACHKXP_002861"
     CDS             1886..2314
                     /gene="rplK"
                     /locus_tag="ACHKXP_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO7647833.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2318..3022
                     /gene="rplA"
                     /locus_tag="ACHKXP_002862"
     CDS             2318..3022
                     /gene="rplA"
                     /locus_tag="ACHKXP_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO7647834.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3435..3932
                     /gene="rplJ"
                     /locus_tag="ACHKXP_002863"
     CDS             3435..3932
                     /gene="rplJ"
                     /locus_tag="ACHKXP_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO7647835.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3999..4364
                     /gene="rplL"
                     /locus_tag="ACHKXP_002864"
     CDS             3999..4364
                     /gene="rplL"
                     /locus_tag="ACHKXP_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO7647836.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4684..8712
                     /gene="rpoB"
                     /locus_tag="ACHKXP_002865"
     CDS             4684..8712
                     /gene="rpoB"
                     /locus_tag="ACHKXP_002865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO7647837.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8789..13012
                     /gene="rpoC"
                     /locus_tag="ACHKXP_002866"
     CDS             8789..13012
                     /gene="rpoC"
                     /locus_tag="ACHKXP_002866"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO7647838.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13225..13764
                     /gene="yjaZ"
                     /locus_tag="ACHKXP_002867"
     CDS             13225..13764
                     /gene="yjaZ"
                     /locus_tag="ACHKXP_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO7647839.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14174..15307)
                     /gene="thiH"
                     /locus_tag="ACHKXP_002868"
     CDS             complement(14174..15307)
                     /gene="thiH"
                     /locus_tag="ACHKXP_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO7647840.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDSYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15304..16074)
                     /gene="thiG"
                     /locus_tag="ACHKXP_002869"
     CDS             complement(15304..16074)
                     /gene="thiG"
                     /locus_tag="ACHKXP_002869"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO7647841.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(16076..16276)
                     /gene="thiS"
                     /locus_tag="ACHKXP_002870"
     CDS             complement(16076..16276)
                     /gene="thiS"
                     /locus_tag="ACHKXP_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO7647842.1"
                     /translation="MQILFNDQAMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16260..17015)
                     /gene="thiF"
                     /locus_tag="ACHKXP_002871"
     CDS             complement(16260..17015)
                     /gene="thiF"
                     /locus_tag="ACHKXP_002871"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO7647843.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(17008..17643)
                     /gene="thiE"
                     /locus_tag="ACHKXP_002872"
     CDS             complement(17008..17643)
                     /gene="thiE"
                     /locus_tag="ACHKXP_002872"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO7647844.1"
                     /translation="MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17643..19538)
                     /gene="thiC"
                     /locus_tag="ACHKXP_002873"
     CDS             complement(17643..19538)
                     /gene="thiC"
                     /locus_tag="ACHKXP_002873"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO7647845.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19619..19719)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19771..20247)
                     /gene="rsd"
                     /locus_tag="ACHKXP_002874"
     CDS             complement(19771..20247)
                     /gene="rsd"
                     /locus_tag="ACHKXP_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO7647846.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20342..21115
                     /gene="nudC"
                     /locus_tag="ACHKXP_002875"
     CDS             20342..21115
                     /gene="nudC"
                     /locus_tag="ACHKXP_002875"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO7647847.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21155..22219
                     /gene="hemE"
                     /locus_tag="ACHKXP_002876"
     CDS             21155..22219
                     /gene="hemE"
                     /locus_tag="ACHKXP_002876"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO7647848.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22229..22900
                     /gene="nfi"
                     /locus_tag="ACHKXP_002877"
     CDS             22229..22900
                     /gene="nfi"
                     /locus_tag="ACHKXP_002877"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO7647849.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22943..23533
                     /gene="yjaG"
                     /locus_tag="ACHKXP_002878"
     CDS             22943..23533
                     /gene="yjaG"
                     /locus_tag="ACHKXP_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO7647850.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23720..23992
                     /gene="hupA"
                     /locus_tag="ACHKXP_002879"
     CDS             23720..23992
                     /gene="hupA"
                     /locus_tag="ACHKXP_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO7647851.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24005..24700
                     /gene="yjaH"
                     /locus_tag="ACHKXP_002880"
     CDS             24005..24700
                     /gene="yjaH"
                     /locus_tag="ACHKXP_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO7647852.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24702..25112)
                     /gene="zraP"
                     /locus_tag="ACHKXP_002881"
     CDS             complement(24702..25112)
                     /gene="zraP"
                     /locus_tag="ACHKXP_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO7647853.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGGGHMGMGHW"
     gene            25350..26726
                     /gene="zraS"
                     /locus_tag="ACHKXP_002882"
     CDS             25350..26726
                     /gene="zraS"
                     /locus_tag="ACHKXP_002882"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO7647854.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPVIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAAGQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLCLPVN
                     ITRKDPQG"
     gene            26723..28048
                     /gene="zraR"
                     /locus_tag="ACHKXP_002883"
     CDS             26723..28048
                     /gene="zraR"
                     /locus_tag="ACHKXP_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO7647855.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28045..29334)
                     /gene="purD"
                     /locus_tag="ACHKXP_002884"
     CDS             complement(28045..29334)
                     /gene="purD"
                     /locus_tag="ACHKXP_002884"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO7647856.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVDTVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29346..30935)
                     /gene="purH"
                     /locus_tag="ACHKXP_002885"
     CDS             complement(29346..30935)
                     /gene="purH"
                     /locus_tag="ACHKXP_002885"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO7647857.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7432 a         8130 c         8605 g         7294 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggccgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccgtac cgttggcgcg
      541 ggcgttgtag caaaagttct gagctaattg ccgataacat ttgacgcaat gcgcactaaa
      601 agggcatcat ttgatgccct ttttgcacgc tttcgtacca gaacctggct catcagtgat
      661 tttctttgtc ataatcattg ctgagacagg ctctgttgag ggcgtataat ccgaaaagct
      721 aatacgcgtt tcgatttggt ttgcctcgcg atcgcggggt gaaaatgttt gtagaaaact
      781 tctgacaggt tggtttatga gtgcgaatac cgaagctcaa ggaagcgggc gcggcctgga
      841 agcgatgaag tgggtcgttg tggtggcatt gctcctggtg gcgattgtcg gcaactatct
      901 ttatcgcgac attatgctgc cgctgcgtgc gctggccgta gtaattctga ttgctgcagc
      961 gggtggtgtc gcgctgttaa cgacaaaagg taaagctacc gttgcttttg cccgtgaagc
     1021 gcgtaccgaa gtccgtaagg tcatttggcc gactcgccag gaaacattgc acaccacgct
     1081 gattgtggct gcggttaccg cagtaatgtc actgatcctg tggggactgg atggtattct
     1141 ggttcgcctg gtatccttta tcactggcct gaggttctga gatgtctgaa gctcctaaaa
     1201 agcgctggta cgtcgttcag gcgttttccg gttttgaagg ccgcgtagca acgtcgctgc
     1261 gtgagcatat caaattacac aacatggaag atttgtttgg tgaagtcatg gtaccaaccg
     1321 aagaagtggt tgaaatccgt ggcggtcagc gtcgcaaaag cgaacgtaaa ttcttccctg
     1381 gctacgtcct cgttcagatg gtgatgaacg acgcgagctg gcacctggtg cgcagcgtac
     1441 cgcgtgtgat gggcttcatc ggcggtactt ccgatcgtcc tgcgccaatc agcgataaag
     1501 aagtcgatgc gattatgaac cgcctgcagc aggttggtga taagccgcgt ccgaaaacgc
     1561 tgtttgaacc gggtgaaatg gtccgtgtta atgatggtcc gttcgctgac ttcaacggtg
     1621 ttgttgaaga agtggattac gagaaatctc gtctgaaagt gtctgtttct atcttcggtc
     1681 gtgcgacccc ggtagagctg gacttcagcc aggttgaaaa agcctaaccc agcgatcaaa
     1741 aaagcggcga tttaatcgtt gcacaaggcg tgagattgga atacaatttc gcgccttttg
     1801 tttttatggg ccttgcccgt aaaacgattt tttatatcac ggggagcctc tcagaggcgt
     1861 tattacccaa cttgaggaat ttataatggc taagaaagta caagcctatg tcaagctgca
     1921 ggttgcagct ggtatggcta acccgagtcc gccagtaggt ccggctctgg gtcagcaggg
     1981 cgtaaacatc atggaattct gcaaagcgtt caacgcaaaa actgattcca tcgaaaaagg
     2041 tctgccgatt ccggtagtaa tcaccgttta cgctgaccgt tctttcactt tcgttaccaa
     2101 gaccccgccg gcagcagttc tgctgaaaaa agcggctggt atcaagtctg gttccggtaa
     2161 gccgaacaaa gacaaagtgg gtaaaatttc ccgcgctcag ctgcaggaaa tcgcgcagac
     2221 caaagctgcc gacatgactg gtgccgacat tgaagcgatg actcgctcca tcgaaggtac
     2281 tgcacgttcc atgggcctgg tagtggagga ctaagaaatg gctaaactga ccaagcgcat
     2341 gcgtgttatc cgcgagaaag ttgatgcaac caaacagtac gacatcaacg aagctatcgc
     2401 actgctgaaa gagctggcga ctgctaaatt cgtagaaagc gtggacgtag ctgttaacct
     2461 cggcatcgac gctcgtaaat ctgaccagaa cgtacgtggt gcaactgtac tgccgcacgg
     2521 tactggccgt tccgttcgcg tagccgtatt tacccaaggt gcaaacgctg aagctgctaa
     2581 agctgcaggc gcagaactgg taggtatgga agatctggct gaccagatca agaaaggcga
     2641 aatgaacttt gacgttgtta ttgcttctcc ggatgcaatg cgcgttgttg gccagctggg
     2701 ccaggttctg ggtccgcgcg gcctgatgcc aaacccgaaa gtgggtactg taacaccgaa
     2761 cgttgctgaa gcggttaaaa acgctaaagc tggccaggtt cgttaccgta acgacaaaaa
     2821 cggcatcatc cacaccacca tcggtaaagt ggactttgac gctgacaaac tgaaagaaaa
     2881 cctggaagct ctgctggttg cgctgaaaaa agcaaaaccg actcaggcga aaggcgtgta
     2941 catcaagaaa gttagcatct ccaccaccat gggtgcaggt gttgcagttg accaggctgg
     3001 cctgagcgct tctgtaaact aatgccttta cgtgggcggt gattttgtct acaatcttac
     3061 ccccacgtat aatgcttaat gcagacgtat atccgagata ttcgggttgt ggcaaggcgg
     3121 caactgagtg agtcgccagg agcatagcta actatgtgac tggtgcgaat gaaggaagcc
     3181 aacgccgtca caagctgaat agcgacggat agaaaagatt tgttcgttgg agcctggcct
     3241 atccaggcct ccgtcgaaga ccgcaggagt ttcgcaagaa acttaatccc ctgcgtagac
     3301 ggtgacagaa cgctaagatt attcttttat attctggctt gtttctgctc accgtaatta
     3361 agacgctctc tccgtttgga ggagtgaagt gagttccaga gattttctct ggcaaacatc
     3421 caggagcaaa gctaatggct ttaaatcttc aagacaaaca agcgattgtt gctgaagtca
     3481 gcgaagtagc caaaggcgcg ctgtctgcag tagttgcgga ttcccgtggc gtaactgtag
     3541 ataaaatgac tgaactgcgt aaagcaggtc gcgaagctgg cgtatacatg cgtgttgttc
     3601 gtaacaccct gctgcgccgt gctgttgaag gtactccgtt cgagtgcctg aaagacgcgt
     3661 ttgttggtcc gaccctgatt gcatactcta tggaacaccc gggcgctgct gctcgtctgt
     3721 tcaaagagtt cgcgaaagcg aatgcaaaat ttgaggtcaa agccgctgcc tttgaaggtg
     3781 agctgatccc ggcgtctcag atcgaccgcc tggcaactct gccgacctac gaagaagcaa
     3841 ttgcacgcct gatggcaacc atgaaagaag cttcggctgg caaactggtt cgtactctgg
     3901 ctgctgtacg cgatgcgaaa gaagctgctt aatcgcagtt atctttttaa cgcattcgct
     3961 tacgtataaa cttattctga tattcaggaa caatttaaat gtctatcact aaagatcaaa
     4021 tcattgaagc agttgcagct atgtctgtaa tggacgttgt agaactgatc tctgcaatgg
     4081 aagaaaaatt cggtgtttcc gctgctgctg ctgtagctgt agctgctggc ccggttgaag
     4141 ctgctgaaga aaaaactgaa ttcgacgtaa ttctgaaagc tgctggcgct aacaaagttg
     4201 ctgttatcaa agcagtacgt ggcgcaactg gcctgggtct gaaagaagct aaagacctgg
     4261 tagaatctgc accggctgct ctgaaagaag gcgtgagcaa agacgacgca gaagcactga
     4321 aaaaagctct ggaagaagct ggcgctgaag ttgaagttaa ataagccaac ccttccggtt
     4381 gcagcctgag aaatcaggct gatggctggt gactttttag tcaccagcct ttttgcgctg
     4441 taaggcgcca gtagcgtttc acactgtttg actactgctg tgcctttcaa tgcttgtttc
     4501 tatcgacgac ttaatatact gcgacaggac gtccgttctg tgtaaatcgc aatgaaatgg
     4561 tttaagcgtg atagcaacag gcattgcgga aagtgttcca ttttccggtc aacaaaatag
     4621 tgttgcacaa actgtccgct caatggacag atgggtcgac ttgtcagcga gctgaggaac
     4681 cctatggttt actcctatac cgagaaaaaa cgtattcgta aggattttgg taaacgtcca
     4741 caagttctgg atgtacctta tctcctttct atccagcttg actcgtttca gaaatttatc
     4801 gagcaagatc ctgaagggca gtatggtctg gaagctgctt tccgttccgt attcccgatt
     4861 cagagctaca gcggtaattc cgagctgcaa tacgtcagct accgccttgg cgaaccggtg
     4921 tttgacgtcc aggaatgtca aatccgtggc gtgacctatt ccgcaccgct gcgcgttaaa
     4981 ctgcgtctgg tgatctatga gcgcgaagcg ccggaaggca ccgtaaaaga cattaaagaa
     5041 caagaagtct acatgggcga aattccgctc atgacagaca acggtacctt tgttatcaac
     5101 ggtactgagc gtgttatcgt ttcccagctg caccgtagtc cgggcgtctt ctttgactcc
     5161 gacaaaggta aaacccactc ttcgggtaaa gtgctgtata acgcgcgtat catcccttac
     5221 cgtggttcct ggctggactt cgaattcgat ccgaaggaca acctgttcgt acgtatcgac
     5281 cgtcgccgta aactgcctgc gaccatcatt ctgcgcgccc tgaactacac cacagagcag
     5341 atcctcgacc tgttctttga aaaagttatc tttgaaatcc gtgataacaa gctgcagatg
     5401 gaactggtgc cggaacgcct gcgtggtgaa accgcatctt ttgacatcga agctaacggt
     5461 aaagtgtacg tagaaaaagg ccgccgtatc actgcgcgcc acattcgcca gctggaaaaa
     5521 gacgacgtca aactgatcga agtcccggtt gagtacatcg caggtaaagt ggttgctaaa
     5581 gactatattg atgagtctac cggcgagctg atctgcgcag cgaacatgga gctgagcctg
     5641 gatctgctgg ctaagctgag ccagtctggt cacaagcgta tcgaaacgct gttcaccaac
     5701 gatctggatc acggcccata tatctctgaa accttacgtg tcgacccaac taacgaccgt
     5761 ctgagcgcac tggtagaaat ctaccgcatg atgcgccctg gcgagccgcc gactcgtgaa
     5821 gcagctgaaa gcctgttcga gaacctgttc ttctccgaag accgttatga cttgtctgcg
     5881 gttggtcgta tgaagttcaa ccgttctctg ctgcgcgaag aaatcgaagg ttccggtatc
     5941 ctgagcaaag acgacatcat tgatgttatg aaaaagctca tcgatatccg taacggtaaa
     6001 ggcgaagtcg atgatatcga ccacctcggc aaccgtcgta tccgttccgt tggcgaaatg
     6061 gcggaaaacc agttccgcgt tggcctggta cgtgtagagc gtgcggtgaa agagcgtctg
     6121 tctctgggcg atctggatac cctgatgcca caggatatga tcaacgccaa gccgatttcc
     6181 gcagcagtga aagagttctt cggttccagc cagctgtctc agtttatgga ccagaacaac
     6241 ccgctgtctg agattacgca caaacgtcgt atctccgcac tcggcccagg cggtctgacc
     6301 cgtgaacgtg caggcttcga agttcgagac gtacacccga ctcactacgg tcgcgtatgt
     6361 ccaatcgaaa cccctgaagg tccgaacatc ggtctgatca actctctgtc cgtgtacgca
     6421 cagactaacg aatacggctt ccttgagact ccgtatcgta aagtgaccga cggtgttgta
     6481 actgacgaaa ttcactacct gtctgctatc gaagaaggca actacgttat cgcccaggcg
     6541 aactccaact tggatgaaga aggccacttc gtagaagacc tggtaacttg ccgtagcaaa
     6601 ggcgaatcca gcttgttcag ccgcgaccag gttgactaca tggacgtatc cacccagcag
     6661 gtggtatccg tcggtgcgtc cctgatcccg ttcctggaac acgatgacgc caaccgtgca
     6721 ttgatgggtg cgaacatgca acgtcaggcc gttccgactc tgcgcgctga taagccgctg
     6781 gttggtactg gtatggaacg tgctgttgcc gttgactccg gtgtaactgc ggtagctaaa
     6841 cgtggtggtg tcgttcagta cgtggatgct tcccgtatcg ttatcaaagt taacgaagac
     6901 gagatgtatc cgggtgaagc aggtatcgac atctacaacc tgaccaaata cacccgttct
     6961 aaccagaaca cctgtatcaa ccagatgccg tgtgtgtctc tgggtgaacc ggttgaacgt
     7021 ggcgacgtgc tggcagacgg tccgtccacc gacctcggtg aactggcgct tggtcagaac
     7081 atgcgcgtag cgttcatgcc gtggaatggt tacaacttcg aagactccat cctcgtatcc
     7141 gagcgtgttg ttcaggaaga ccgtttcacc accatccaca ttcaggaact ggcgtgtgtg
     7201 tcccgtgaca ccaagctggg tccggaagag atcaccgctg acatcccgaa cgtgggtgaa
     7261 gctgcgctct ccaaactgga tgaatccggt atcgtttaca ttggtgcgga agtgaccggt
     7321 ggcgacattc tggttggtaa ggtaacgccg aaaggtgaaa ctcagctgac cccagaagaa
     7381 aaactgctgc gtgcgatctt cggtgagaaa gcctctgacg ttaaagactc ttctctgcgc
     7441 gtaccaaacg gtgtatccgg tacggttatc gacgttcagg tctttactcg cgatggcgta
     7501 gaaaaagaca aacgtgcgct ggaaatcgaa gaaatgcagc tcaaacaggc gaagaaagac
     7561 ctgtctgaag aactgcagat cctcgaagcg ggtctgttca gccgtatccg tgctgtgctg
     7621 gtagccggtg gcgttgaagc tgagaagctc gacaaactgc cgcgcgatcg ctggctggag
     7681 ctgggcctga cagacgaaga gaaacaaaat cagctggaac agctggctga gcagtatgac
     7741 gaactgaaac acgagttcga gaagaaactc gaagcgaaac gccgcaaaat cacccagggc
     7801 gacgatctgg caccgggcgt gctgaagatt gttaaggtat atctggcggt taaacgccgt
     7861 atccagcctg gtgacaagat ggcaggtcgt cacggtaaca agggtgtaat ttctaagatc
     7921 aacccgatcg aagatatgcc ttacgatgaa aacggtacgc cggtagacat cgtactgaac
     7981 ccgctgggcg taccgtctcg tatgaacatc ggtcagatcc tcgaaaccca cctgggtatg
     8041 gctgcgaaag gtatcggcga caagatcaac gccatgctga aacagcagca agaagtcgcg
     8101 aaactgcgcg aattcatcca gcgtgcgtac gatctgggcg ctgacgttcg tcagaaagtt
     8161 gacctgagta ccttcagcga tgaagaagtt atgcgtctgg ctgaaaacct gcgcaaaggt
     8221 atgccaatcg caacgccggt gttcgacggt gcgaaagaag cagaaattaa agagctgctg
     8281 aaacttggcg acctgccgac ttccggtcag atccgcctgt acgatggtcg cactggtgaa
     8341 cagttcgagc gtccggtaac cgttggttac atgtacatgc tgaaactgaa ccacctggtc
     8401 gacgacaaga tgcacgcgcg ttccaccggt tcttacagcc tggttactca gcagccgctg
     8461 ggtggtaagg cacagttcgg tggtcagcgt ttcggggaga tggaagtgtg ggcgctggaa
     8521 gcatacggcg cagcatacac cctgcaggaa atgctcaccg ttaagtctga tgacgtgaac
     8581 ggtcgtacca agatgtataa aaacatcgtg gacggcaacc atcagatgga gccgggcatg
     8641 ccagaatcct tcaacgtatt gttgaaagag attcgttcgc tgggtatcaa catcgaactg
     8701 gaagacgagt aattctcgct caaacaggtc actgctgtcg ggttaaaacc cggcagcgga
     8761 ttgtgctaac tccgacggga gcaaatccgt gaaagattta ttaaagtttc tgaaagcgca
     8821 gactaaaacc gaagagtttg atgcgatcaa aattgctctg gcttcgccag acatgatccg
     8881 ttcatggtct ttcggtgaag ttaaaaagcc ggaaaccatc aactaccgta cgttcaaacc
     8941 agaacgtgac ggccttttct gcgcccgtat ctttgggccg gtaaaagatt acgagtgcct
     9001 gtgcggtaag tacaagcgcc tgaaacaccg tggcgtcatc tgtgagaagt gcggcgttga
     9061 agtgacccag actaaagtac gccgtgagcg tatgggccac atcgaactgg cttccccgac
     9121 tgcgcacatc tggttcctga aatcgctgcc gtcccgtatc ggtctgctgc tcgatatgcc
     9181 gctgcgcgat atcgaacgcg tactgtactt tgaatcctat gtggttatcg aaggcggtat
     9241 gaccaacctg gaacgtcagc agatcctgac tgaagagcag tatctggacg cgctggaaga
     9301 gttcggtgac gaattcgacg cgaagatggg ggcggaagca atccaggctc tgctgaagag
     9361 catggatctg gagcaagagt gcgaacagct gcgtgaagag ctgaacgaaa ccaactccga
     9421 aaccaagcgt aaaaagctga ccaagcgtat caaactgctg gaagcgttcg ttcagtctgg
     9481 taacaaacca gagtggatga tcctgaccgt tctgccggta ctgccgccag atctgcgtcc
     9541 gctggttccg ctggatggtg gtcgtttcgc gacttctgac ctgaacgatc tgtatcgtcg
     9601 cgtcattaac cgtaacaacc gtctgaaacg tctgctggat ctggctgcgc cggacatcat
     9661 cgtacgtaac gaaaaacgta tgctgcagga agcggtagac gccctgctgg ataacggtcg
     9721 tcgcggtcgt gcgatcaccg gttctaacaa gcgtcctctg aaatctttgg ccgacatgat
     9781 caaaggtaaa cagggtcgtt tccgtcagaa cctgctcggt aagcgtgttg actactccgg
     9841 tcgttctgta atcaccgtag gtccatacct gcgtctgcat cagtgcggtc tgccgaagaa
     9901 aatggcactg gagctgttca aaccgttcat ctacggcaag ctggaactgc gtggtcttgc
     9961 taccaccatt aaagctgcga agaaaatggt tgagcgcgaa gaagctgtcg tttgggatat
    10021 cctggacgaa gttatccgcg aacacccggt actgctgaac cgtgcaccga ctctgcaccg
    10081 tctgggtatc caggcatttg aaccggtact gatcgaaggt aaagctatcc agctgcaccc
    10141 gctggtttgt gcggcatata acgccgactt cgatggtgac cagatggctg ttcacgtacc
    10201 gctgacgctg gaagcccagc tggaagcgcg tgcgctgatg atgtctacca acaacatcct
    10261 gtccccggcg aacggcgaac caatcatcgt tccgtctcag gacgttgtac tgggtctgta
    10321 ctacatgacc cgtgactgtg ttaacgccaa aggcgaaggc atggtgctga ctggcccgaa
    10381 agaagcagaa cgtctgtatc gctctggtct ggcttctctg catgcgcgcg ttaaagtgcg
    10441 tatcaccgag tatgaaaaag atgctaacgg tgaattagta gcgaaaacca gcctgaaaga
    10501 cacgactgtt ggccgtgcca ttctgtggat gattgtaccg aaaggtctgc cttactccat
    10561 cgtcaaccag gcgctgggta aaaaagcaat ctccaaaatg ctgaacacct gctaccgcat
    10621 tctcggtctg aaaccgaccg ttatttttgc ggaccagatc atgtacaccg gcttcgccta
    10681 tgcagcgcgt tctggtgcat ctgttggtat cgatgacatg gtcatcccgg agaagaaaca
    10741 cgaaatcatc tccgaggcag aagcagaagt tgctgaaatt caggagcagt tccagtctgg
    10801 tctggtaact gcgggcgaac gctacaacaa agttatcgat atctgggctg cggcgaacga
    10861 tcgtgtatcc aaagcgatga tggataacct gcaaactgaa accgtgatta accgtgacgg
    10921 tcaggaagag aagcaggttt ccttcaacag catctacatg atggccgact ccggtgcgcg
    10981 tggttctgcg gcacagattc gtcagcttgc tggtatgcgt ggtctgatgg cgaagccgga
    11041 tggctccatc atcgaaacgc caatcaccgc gaacttccgt gaaggtctga acgtactcca
    11101 gtacttcatc tccacccacg gtgctcgtaa aggtctggcg gataccgcac tgaaaactgc
    11161 gaactccggt tacctgactc gtcgtctggt tgacgtggcg caggacctgg tggttaccga
    11221 agacgattgt ggtacccatg aaggtatcat gatgactccg gttatcgagg gtggtgacgt
    11281 taaagagccg ctgcgcgatc gcgtactggg tcgtgtaact gctgaagacg ttctgaagcc
    11341 gggtactgct gatatcctcg ttccgcgcaa cacgctgctg cacgaacagt ggtgtgacct
    11401 gctggaagag aactctgtcg acgcggttaa agtacgttct gttgtatctt gtgacaccga
    11461 ctttggtgta tgtgcgcact gctacggtcg tgacctggcg cgtggccaca tcatcaacaa
    11521 gggtgaagca atcggtgtta tcgcggcaca gtccatcggt gaaccgggta cacagctgac
    11581 catgcgtacg ttccacatcg gtggtgcggc atctcgtgcg gctgctgaat ccagcatcca
    11641 agtgaaaaac aaaggtagca tcaagctcag caacgtgaag tcggttgtga actccagcgg
    11701 taaactggtt atcacttccc gtaatactga actgaaactg atcgacgaat tcggtcgtac
    11761 taaagaaagc tacaaagtac cttacggtgc ggtactggcg aaaggcgatg gcgaacaggt
    11821 tgctggcggc gaaaccgttg caaactggga cccgcacacc atgccggtta tcaccgaagt
    11881 aagcggtttt gtacgcttta ctgacatgat cgacggccag accattacgc gtcagaccga
    11941 cgaactgacc ggtctgtctt cgctggtggt tctggattcc gcagaacgta ccgcaggtgg
    12001 taaagatctg cgtccggcac tgaaaatcgt tgatgctcag ggtaacgacg ttctgatccc
    12061 aggtaccgat atgccagcgc agtacttcct gccgggtaaa gcgattgttc agctggaaga
    12121 tggcgtacag atcagctctg gtgacaccct ggcgcgtatt ccgcaggaat ccggcggtac
    12181 caaggacatc accggtggtc tgccgcgcgt tgcggacctg ttcgaagcac gtcgtccgaa
    12241 agagccggca atcctggctg aaatcagcgg tatcgtttcc ttcggtaaag aaaccaaagg
    12301 taaacgtcgt ctggttatca ccccggtaga cggtagcgat ccgtacgaag agatgattcc
    12361 gaaatggcgt cagctcaacg tgttcgaagg tgaacgtgta gaacgtggtg acgtaatttc
    12421 cgacggtccg gaagcgccgc acgacattct gcgtctgcgt ggtgttcatg ctgttactcg
    12481 ttacatcgtt aacgaagtac aggacgtata ccgtctgcag ggcgttaaga ttaacgataa
    12541 acacatcgaa gttatcgttc gtcagatgct gcgtaaagct accatcgtta acgcgggtag
    12601 ctccgacttc ctggaaggcg aacaggttga atactctcgc gtcaagatcg caaaccgcga
    12661 actggaagcg aacggcaaag tgggtgcaac ttactcccgc gatctgctgg gtatcaccaa
    12721 agcgtctctg gcaaccgagt ccttcatctc cgcggcatcg ttccaggaga ccactcgcgt
    12781 gctgaccgaa gcagccgttg cgggcaaacg cgacgaactg cgcggcctga aagagaacgt
    12841 tatcgtgggt cgtctgatcc cggcaggtac cggttacgcg taccaccagg atcgtatgcg
    12901 tcgccgtgct gcgggtgaag ctccggctgc accgcaggtg actgcagaag acgcatctgc
    12961 cagcctggca gaactgctga acgcaggtct gggcggttct gataacgagt aatcgttaat
    13021 ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag
    13081 agcatttgtc agaatattta aggaatttct gaatactcat aatcaatgta gagatgacta
    13141 atatcctgaa actgactgaa ctaattgagt caaactcggc aaggattcga tactattcct
    13201 gtgtaacttt cttaaggaac gagaatgaaa caggaagtgg aaaagtggcg accttttgga
    13261 catccggatg gtgatattcg tgatttatca tttcttgatg ctcatcaggc tgtctacgtt
    13321 cagcatcatg agggcaaaga gcctttagag tatcgctttt gggttaccta ctctcttcac
    13381 tgcttcacaa aagattatga acatcagacg aacgaagaaa aacaatcgtt aatgtaccac
    13441 gcgcctaaag aatctcgtcc cttctgccag caccgttata acttagcgcg cacacactta
    13501 aaaagaacta ttttggcgct gccagaaagc aacgttattc atgccgggta tggtagctat
    13561 gccgtgattg aggtggactt agacggagga gataaggcat tttactttgt tgcgttcagg
    13621 gctttcaggg aaaagaaaaa actccgtttg catgtaacta gcgcttatcc catttctgaa
    13681 aaacagaaag gtaaatcagt gaaatttttc accattgcct acaacttatt gagaaataag
    13741 cagcttcctc agccctcaaa ataacaaaac ccaccttaag gtgggtttcg ccagagaatt
    13801 atctctggta ttcagaacgc cattaccgga ctttgccttg accttgcgat aatcgcaggt
    13861 tgcgggatgt ctgaatttct tcagtctgct gcatcctgga agatgagaac atgtgttctt
    13921 attttcgtct ctatcatagt tgagtattta ctctcttaca atcagatctc tttcattgct
    13981 caacaggcga tggcttcaga ctttgcatta cggaattttt aagaaaggca gggcgaaacg
    14041 aggaagaagc ttttcgccct ggacaacatt cctgctgacg gcactaccat aaaaaaacac
    14101 aacgctttca acaaccttgc gtttttcatc ttccgtagcg gctcggaata aatttacgtt
    14161 cttacattcc gtctcatagt ctttgcgagg cgcgtcccag atagctgtcc cagtctttcc
    14221 ataccggctg caaaccctga gcggttaacg cggcagcaac cgcttccggt ctgcgatcgt
    14281 cgtgcggtga gaactgttcc aactcggggt gattatcggc atagccacct ggctgcgttt
    14341 tcgagaaggc gctgacgtta ttgatcgcca gcggaataac gcgatcgcga aaccacggtg
    14401 attcccgcgt ggagagtgac agttcaatct ccggtgcaag cagtcggaag gcgcagatgg
    14461 tttgcactaa ctggcgttca tccataatcg acgcaggctc aatgccgcca gtacacgggc
    14521 gcaggcgcgg aaaggagaca gagtaacggc tttgccagta atgctgttgc agccatagca
    14581 aatgttcggc aaccatatag ctgtcaacgc gccagttgtc ggaaaggcca attagcgcgc
    14641 cgaggcctat cttatcaatc cccgcacgcc ccagccgatc cggcgtttcc agccgccaga
    14701 agaagtcctg ttttttgcct ttcagatgat ggcgggcata agtcgcctcg tgatatgtct
    14761 cctgataaac catcacgcca tccagaccaa gttgctttaa ctcggcgtat tccgtctccg
    14821 ccagcggttg cacttccatc tgtagtgaag agaactgttc acgaagggca gggagatgac
    14881 gacgaaagta atccatcccc actttcgcct gatgttcacc agtgactaac agcagatgtt
    14941 caaagcccat ctcccgtata gcggcacttt ccctggcaat atccgcttca tccagcgttt
    15001 tgcgcttgat gcgattactc atggaaaatc cacagtacgt gcagtcgtta gcgcaaagat
    15061 tggaaagata aagcgggacg tagaaactaa ctgtgttgcc aaatcgctga cgggtcagac
    15121 gctgcgcccg ttgggccagt tgttccagat agccactggc ggcaggcgat aacagcgcca
    15181 tcatgtcgtc gcgggtgagt tgcgaggcat ttagcgcccg ctctacgtca gcagccgttt
    15241 tgccgttgat acgcaggcgg atgtcgtccc agtccagttg tcgccagcga tcgctgaagg
    15301 ttttcatgcc gatgcctcca gaaatccggt cagcgggctg gtggcatgag caaaataact
    15361 gcggctgccc ggtccggact gacgtgccag taggcctgct tctaccgcca gacgaaatgc
    15421 cttcgccatg ttgacgggat cgtccgcgac ggcaatcgcc gtattcacta acaccgcgtc
    15481 ggcccccatt tccagcgcct gcgcggcatg gctgggaacg ccgatgccag catcgacaac
    15541 caccggcact gtggcctgct ggataataat ctccagcatg gcgcgggttt ccagtccctg
    15601 attcgagcca atcggcgcgc cgagcggcat caccgctgca cagccgactt cttccagacg
    15661 tttacacaat accggatcgg ccccgcagta aggcagcacg acaaatccct gttgtaccag
    15721 cgtttcggcg gctttcaggg tttcgatggg atcgggcaac agccagcggg cgtcagggtg
    15781 aatctctaat tttaaccagt ttgtgcctaa cgcttcacga gccagatggg cggcgaaaat
    15841 ggcttcttcc gctgttttcg ccccggatgt atttggcagc agggtcacac ccgccgcgat
    15901 aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg acacgtttca tcgccagtgt
    15961 caccagctgg ctgccggaag cgcggatcgc ctccaccatc agttgtgaag aagcgaattt
    16021 ccctgtgccg gtaaacagat gtgaatcaaa cgttttgtcc gcaatacgta acatttcaac
    16081 cccctgcaat aacctgaaaa agcaggatct ggtcgccatc ctgcacgata tgttgcgccc
    16141 actgctcacg cgggacgatt tgctgattaa tcgccagagc cgcgcccgct tgtcgttggt
    16201 cgagttgctc cagtagttcg tgaacagttt gcccggcggc gcactgcatc gcttgatcgt
    16261 taaacaggat ctgcattgct tcctccgcat accgggcaac cactggcgcg gcgcaacgcc
    16321 aggctgcgcc actggctcga tttaccgtcg aacagtcgga gttctcccgc aggtgtctct
    16381 ataccgctta ataacttaat ggcttccagt gcctgcaaag tgcccataac cccgaccacc
    16441 gggccaacca cgcccgccgt gcggcagttg cgttctggct cctggttatc tggccacagg
    16501 cagcggtaac acccctgctc ccagggcggc gtcagtacca tcaactgacc gccaaatccg
    16561 accgcgctgg cggtgataag cggcgtgttg agtgccacgc aggcggcatt aatctcctgg
    16621 cgagtcgcca tattgtcggt acagtcgagc accacatcgg cccgtgcaac cgcatctttt
    16681 aacgcctcac ccgttaaccg ttgttgtaat gctgtcagtt gaatgtcggg attcaactgt
    16741 gtcagtcgct gttggctgac ctgcgatttc gggcgatcga tatcttcagt ggtaaagagg
    16801 atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat ctgccagtac cagcgtcccg
    16861 acgccagcgc ccgccaggta cagcgcagca ggtgtaccca gcccgcccag accgataatc
    16921 agcacctggc tgtctagcag tttttgctgc ccgtccagag cgatatcgtc gagcaggatt
    16981 tggcggctat aacgcataaa gtcacggtca ttcatcgcca actcctgcaa tttccagcaa
    17041 ctgtgccgtt gccaaacgcc agtctgcggc ttgagtaatg gcgctgacga cggcgatact
    17101 gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga ctgataccgc caatcgccac
    17161 ggtgggataa tccgccagtc gctcaacatg ccgtgccagc tgttccagcc cctgcggtgc
    17221 agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc agcgcgatat aagaggggcg
    17281 tgctgccagc gcgacgtcga tttccatatc gtcatgtgtc gaaacgccca gccgcaggcc
    17341 tgccgcgcgg atggcattga gatcggtggc ttgcaaatct tcctgcccca aatggacgcc
    17401 atacgcctga tgcttgatcg ccagccgcca gtaatcgttg ataaacaatc gcgcgttata
    17461 gcggcggccc agcgcaattg ccgccacgac atcggcttcc acctcttcat cgcgccgatc
    17521 tttgatgcgt agctggagag tacgtacgcc tgcatccaac agacgttcga tccactgtac
    17581 gctgtccacc accgggtaca gtcctgaacg aaaaggtaca ggaggaaaat caggctgata
    17641 catcacgctt cctccttacg caggtagatt tctccgcctc tggcacggaa gttctccgac
    17701 atatccgcca ttcccatttc aatagtttgc gtggcggcgt aatcacgcac ttcctggctg
    17761 attttcatcg agcagaattt cggcccacac atggagcaaa aatgggcgac tttacctgac
    17821 tcttgcggca gggtttcatc gtgataagcg cgggcggtaa acgggtcgag ggccagatta
    17881 aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca tggcgttatc gcgaatttgc
    17941 gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg caatcttata ggtgataagc
    18001 ccctgcttaa catcttcttt attgggcaga cccagatgct cttttggcgt tacgtaacag
    18061 agcatcgcgc agccaaacca gccaatcatc gccgcaccaa tccccgacgt gaagtggtca
    18121 tagcccggcg caatatcggt agttagcggc cccagagtgt aaaacggcgc ttcgtggcag
    18181 tgctctaact cctcggtcat attgcggcgg atcatctgca tcggcacgtg gcctgggcct
    18241 tcaatcatca cctgcacgtc atattcccag gcaattttgg tcagttcgcc cagcgtatgc
    18301 agctcggcaa actgcgcttc atcgttggcg tcctgaatag aaccggggcg cagaccgtcg
    18361 cccagcgaca gcgaaacgtc ataagcggca cagatttcac aaatttcgcg gaagtgttga
    18421 tagaggaaat tttcctgatg atgggagagg caccatttcg ccataatcga accgccgcga
    18481 gagacgatac cggtcaggcg tttcgcggtc atcggcacat agcgcagcag tacgcccgca
    18541 tggatagtga agtaatccac accttgctcg gcctgttcca gcagcgtgtc gcggaacgct
    18601 tcccaggtaa gatcttcggc gatcccgtta accttctcca gcgcctggta gatcggcact
    18661 gtaccgatcg gcaccgggct gttacgcaaa atccactcgc gggtttcgtg aatatagcga
    18721 ccggtggaga gatccatcac cgtatccgct ccccagcgcg tggaccatac cagcttttcc
    18781 acttcttctt cgatggaaga ggtgaccgcc gagttgccga tattggcgtt aacttttacc
    18841 aggaaattgc gaccaataat catcggctcc gattccggat gattaatgtt ggccgggata
    18901 atcgcacgtc cggcagcaac ttcatcacgg acaaattccg cagtgatatt ttccggcaga
    18961 tgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa cctcgctacg gatgcgctcg
    19021 cggcccatat tctcgcggat ggcgatgaat tccatttccg gcgtgatgat gccctggcgg
    19081 gcgtagtgca gttgggtgac acggcgtcct gctttggcgc gttttggtgt tagtacgccg
    19141 ctaaaacgca gttcgtcgag gccatcatct gccagccgcg ctttagtgta atcggaactg
    19201 cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc acggctggcg tagttttgcc
    19261 agcccttgct gcacgttaat ggcaatctgc ggatcaccat acgggccgga ggtgtcgtag
    19321 accggaatcg cttcgttttc ttcgtactgc ggctgttctt tgctaccgcc aattagcgtc
    19381 gggctaagct ggatctcacg catcggcacg cgcacgccgg ggtgtgtgcc agtgatataa
    19441 atgcgttttg agttgggaaa ggcggtgcct tccagggtgt cgataaaatg ttgggcccgg
    19501 gcgcgttgtt cgcggcgggt cagttttgtt gcagacatag ctcattccaa aaagttaagg
    19561 acgtggcttg tcagacgacg gatgaagcaa gagacgatcg ccgcaggggc gatgcgatag
    19621 cagattaact cttgttccct tcgcaggtat tagcctgatc aggttccgcg gatcccgaat
    19681 aaacggtctc agccagttaa ggcactccga caagaaatta gccccgcaaa tggggcattg
    19741 aatgtaaatt acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc
    19801 aagcaccagc agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat
    19861 gtcagataaa acctgttgaa actcaaggta attatcatga tcgatggcgg tttccagact
    19921 ggaatcgtag taatccataa tctgttgagt attggcttcg agttgcggcc aaatcttcgc
    19981 ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa
    20041 atgtccggca gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt
    20101 tagcctcatg tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag
    20161 cagatgctta cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc
    20221 cgtcaggtta tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc
    20281 tcagtgagaa atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc
    20341 tatggatcgt ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa
    20401 attatggttg ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg
    20461 tcagcgcgca ctacaaatcg gcgaatggca gggggaacct gtttggttag tacaacagca
    20521 gcggcgtcac gatatggggt cggtacgtca ggtcattgat ctcgatgttg ggctgtttca
    20581 actggccgga cgaggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta
    20641 ctgcgggcat gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg
    20701 tgagcgttac tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc
    20761 gatcctcctc gcccagcata cccgccatcg taacggtgtc catacggtac ttgccggatt
    20821 cgtcgaagtg ggcgaaaccc tcgagcaggc ggtcgcgcgg gaagtgatgg aagagagcgg
    20881 aattaaagtt aaaaacttgc gttatgtgac ttcccagccg tggccgtttc ctcagtcttt
    20941 aatgaccgcg tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt
    21001 gcttgaggcg aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt
    21061 agcgcgccgt ctgatagaag atactgtggc gatgtgtcgg gcagagtatg agtgatgata
    21121 cactgaccgc ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta
    21181 tctgcgggcg ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc
    21241 gggtcgctat ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct
    21301 gtgcaaaaac gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct
    21361 ggatgcggcg atcctctttt ccgatatcct caccgtgccg gacgcgatgg ggttagggct
    21421 ctattttgaa gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt
    21481 cgataaactg ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg
    21541 taccattcgt cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg
    21601 gacgctggcg acctacatgg tggaaggcgg cagcagcaaa gcgttcaccg tgatcaaaaa
    21661 aatgatgtat gccgatccgc aggcgctgca cgctctactc gataaactgg cgaaaagcgt
    21721 cactttgtat ctgaatgcgc agattaaagc cggtgctcag gcagtgatga ttttcgacac
    21781 ctggggcggt gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt acatgcataa
    21841 aattgttgat ggtttactgc gtgaaaacga cggtcgccgc gtaccggtca cgctgtttac
    21901 caaaggcggc ggacagtggc tggaagcgat ggcagaaacc ggttgcgatg cgttgggcct
    21961 cgactggaca acggatatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca
    22021 gggtaatatg gatccgtcga tgctgtacgc tccgcctgcc cgcattgaag aagaagtagc
    22081 gactatactt gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat
    22141 tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc
    22201 tgaacaatat caccgctaag gagtgattat ggatctcgcg tcattacgcg ctcaacaaat
    22261 cgaactggct tcttctgtga tccgcgagga tcgactcgat aaagatccac cggatctgat
    22321 cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct
    22381 gctgaaatat ccctcgcttg agctggtgga gtataaagtt gcccgcatcg ccaccaccat
    22441 gccttacatt ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga
    22501 gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg
    22561 ccgtcttggc gtcgccagcc attttggctt attggtggat gtgccgacca ttggcgtggc
    22621 gaaaaaacgg ctctgcggta aattcgaacc gctctccagc gaaccgggcg cgctggcccc
    22681 actgatggat aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc
    22741 gttgtttatc gctaccggcc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg
    22801 ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga
    22861 acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa
    22921 tttccgtatt tgagaactca acatgttaca aaacccaatt catctgcgtc tggagcgcct
    22981 ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta
    23041 cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gcattctcga
    23101 tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta aatttcgaca gccaactgga
    23161 gaaatttgaa gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc
    23221 aatcgatgca tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct
    23281 cgaacacgcg gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac
    23341 tcaggctggt cgcgaaatga gcgatgaaga gcttaaagaa aacccagctg tagagcaaga
    23401 atgggacatt cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga
    23461 gctgataaaa ggccttaggg cagacctgcg tgaggcgggt gagagcaata ttggtataat
    23521 ttttcagcaa taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt
    23581 ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt
    23641 gtatgcagga gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa
    23701 cacattgtaa ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc
    23761 agaactgtcc aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga
    23821 gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca
    23881 ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc
    23941 taacgtaccg gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt
    24001 ggcagtgaac agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc
    24061 attgatgctg gcaggtgcgc tgtttctcac tgcctgtagc cacaactctt cacttcctcc
    24121 ctttaccgcc agtggatttg ctgaagacca gggcgcggta cgcatctggc gaaaagacag
    24181 cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac
    24241 gcgagagtat cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa
    24301 accgccggtg aatattcgcg cgcgttttga cgatcgcggt gatctgagct ttatgcaacg
    24361 tgaatccgat ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg
    24421 tgctgatcag atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca
    24481 ggggcgctgg catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc
    24541 tgatttagat tcgcaggcga tagcgcatat cgagcgccgc cagagccgct cttctgttga
    24601 tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa ttactgttgg tggcaaactc
    24661 tgatttctgt cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc
    24721 atatgaccgc caccaccgta acccattccg gtaccgcgcg gaatacccgc ttcagccatc
    24781 gcaatatctc gtttcacccg taactcatct aacgactgac gcaaattctc catctctttg
    24841 gcgaccgcgt taattttgct gctatccggt gggttcgcgg ctaacagggc attgtattca
    24901 taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa gtcattatgg
    24961 attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg ctgccacata
    25021 ccgtgtccgc cgtgagcaaa tgcagatgtc gagcccatcg ccattgctga aagcgccatc
    25081 attaccaggg caattttcgt gttccgtttc atggttaatc ctccgatggt tgtcttactt
    25141 cgggtattgc atcttccgtg ccaacgatga aacgctgata tgacgggtaa tctggcatga
    25201 taaacgagta aaaatgactc gcctgctgcg ggtagcgagt catttttact cattgaaact
    25261 taagcctttg tgttacagcg cagggtaagc gctgataaaa gatggcatga tttctgctgt
    25321 cagaaaggga tgagcaggca aagaagaaga tgcgttttat gcaacgttct aaagactcct
    25381 tagctaaatg gttaagcgcg atcctccccg tggtcattgt tgggctggtg gggctgtttg
    25441 cggtgacggt gattcgcgat tatgggcgcg agactgccgc cgccagacaa acgctgctgg
    25501 aaaaaggcag tgtacttatt cgcgctcttg aatccggctc gcgcgtcggc atggggatgc
    25561 gcatgcatca tgcgcagcag caggccttac tggaagaaat ggccgggcag cctggagtac
    25621 gttggtttgc ggtcacggat gaacaaggaa caatcgtgat gcatagcaac tccggcatgg
    25681 tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt acatccggga gatgaagaag
    25741 cgtggcggcg gatcgatagc gcagacggcg agcctgttct ggaaatttat cgccagtttc
    25801 aaccgatgtt tgctgctgga atgcaccgga tgcgccatat gcagcagtat gccgcgacac
    25861 cacaagcaat tttcatcgct ttcgacgcca gtaatattgt gagtgccgaa gatcgtgagc
    25921 agagaaacac cctgattatc ctcttcgccc tggcgacggt cttgctggca agcgtgttgt
    25981 cattcttctg gtatcgccgc tatctgcgct cgcgccagct gttgcaggat gaaatgaagc
    26041 gcaaagagaa gctggtggca ctggggcatc ttgcagcagg cgttgcccac gaaatccgta
    26101 atccactttc ctcaattaaa gggctggcga aatactttgc cgaacgcgcg ccagcagggg
    26161 gagaagcgca tcaattggcg caggtgatgg cgaaagaagc cgaccgttta aaccgtgtgg
    26221 taagcgagtt gctggaactg gttaagccaa cgcatctggc tttgcaggcg gtggatctca
    26281 acacgctgat taaccactca ttacagctgg taagccagga tgcaaacagc cgggagatcc
    26341 agttacgctt taccgccaac gacacattac cggtaattca ggccgaccca gacaggctga
    26401 ctcaggtcct gttgaatctc tatctcaatg ctattcaggc gattggtcag catggtgtga
    26461 ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat cagcgttacc gacagcggta
    26521 agggaattgc ggcaggtcag cttgaagcca tcttcactcc gtacttcacc accaaagccg
    26581 aaggcaccgg attggggctg gcggtcgtgc ataatattgt tgaacaacac ggtggtacaa
    26641 ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac cctctgtctt ccggtcaata
    26701 ttacgcgtaa ggacccacaa ggatgacgca cgataatatc gatattctgg tggtggatga
    26761 tgacattagc cactgcacta ttttgcaggc tttactgcgc ggctggggct ataacgtcgc
    26821 gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg gagcgagttt ttgatcttgt
    26881 gctttgcgat gtgcgaatgg cggagatgga cggcattgcc acgctaaaag agatcaaagc
    26941 gttaaatccg gcgattccgg tactgattat gaccgcatac tccagcgtcg agacggcggt
    27001 agaggcactg aaaaccgggg cgctggatta tctcatcaag ccgctggatt tcgataacct
    27061 acaggcgacg ctggaaaagg cgctcgcgca tacgcacagt attgatgctg aaacacctgc
    27121 ggtgactgcc agccagttcg gtatggtcgg taaaagcccg gcgatgcaac acctgctcag
    27181 tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg atccacggcg attccggcac
    27241 cggtaaagag ctggtcgcca gggcgattca cgccagtagc gcacgtagcg aaaaaccgct
    27301 ggtaacgctc aactgtgcgg cactcaacga atccttgctg gaatctgaat tgttcggtca
    27361 cgaaaaaggg gcgtttactg gagccgataa acgccgggaa gggcgctttg ttgaggcgga
    27421 cggcggcacg ctgtttctcg atgaaattgg cgatatctcg ccgatgatgc aggtgcgtct
    27481 gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt agcaaccaga ctatctcggt
    27541 tgatgtccgg ctgattgcgg cgacccatcg cgatcttgcc gcagaggtga atgccgggcg
    27601 ttttcgccag gatctctact atcgcctgaa tgtggtggcg attgaagtac catcgctgcg
    27661 gcaacggcgg gaagatatcc ctctgctggc tggccatttt ctgcagcgct ttgccgagcg
    27721 taatcgcaag gcggtaaaag gttttacgcc ccaggcgatg gatctgttga ttcattacga
    27781 ctggccggga aatattcgtg agctggaaaa cgcggtggaa cgggcagtgg tgctgctgac
    27841 cggggaatat atttccgaac gcgagctgcc gctggcgatt gccagtacgc cgatcccgct
    27901 gggacaaagt caggatattc agccgttggt ggaagtggaa aaagaggtga ttctggcggc
    27961 gctggagaaa acgggcggca acaaaaccga agccgcccgt cagttaggga tcacgcgcaa
    28021 aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt tcgatggcgc gccagccgat
    28081 atctttccgg cagaagcagt cgtcccagtg aatatcggtc attaaggcat aagcgcgttt
    28141 ctgcgcttct gccacggtat gacccagcgc ggtgacgcac agtacgcgcc cgccgctggt
    28201 cactacctgc tcgtcatccg ccagttttgt gcctgcatgg aacactttgc cgtcttccac
    28261 ttcttccagc ggcaggccgt ggatcacatc accggtgcgg taatcgcccg gatatccacc
    28321 cgcagccatc accacgccga gagaagcgcg ttcatcccac tcggatgttt tctcgtccag
    28381 cttcccttca caggctgcca ggcagagctc aaccagatcg gacttcatgc gcagcataat
    28441 cggctgggtt tccggatcgc caaagcggca gttaaattcg ataaccttcg gattgccctg
    28501 tttgtcgatc atcaggcccg cgtagagaaa accggtgtag gtgttgcctt ccgccgccat
    28561 gcctttcacg gttggccaga tgatacgttc catggtgcgc tgatgaactt catcggttac
    28621 taccggcgcg ggggagtaag cgcccatccc gccggtgttt ggtccggtat ctttatcgcc
    28681 tacgcgtttg tgatcctggc tggtagccat cggcagcaca tgctctccgt ccaccatcac
    28741 gataaagctc gcttcttcgc catcgaggaa ctcttcgata acgatgcgat gacccgcgtc
    28801 gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct tccgcttctt ccagcgtcat
    28861 cgccacgata acgcctttcc cggcagccag accgtccgct ttaatgacga ttggcgcgcc
    28921 tttctcacgc agatacgcca gcgcaggttc tacctcggtg aagttctggt attccgccgt
    28981 agggatttta tggcgggcca ggaaatcttt ggtaaacgct tttgagcctt ccagttgggc
    29041 cgcacctgct gttgggccga agattttcag cccggcggcg cggaaggtat cgaccacgcc
    29101 tttaaccagc ggcgcttccg ggccgacgat ggtcagatca atcttttcgt tttgtgcgaa
    29161 atccaacagc gccgggatat cggtcacgcc gatagcaacg ttttgcagcg cgggttccag
    29221 cgcagtgcct gcattacccg gagcaacaaa aacagtgtca accagcggcg actgggccgc
    29281 tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc actaatactt tcatctattg
    29341 ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc gtgctcgtcg
    29401 gcggcggcaa tcacttcgtc atcacggatg gaaccgccag gctggattac gcaggtcacg
    29461 cccgcagcgg cggcggcatc aataccgtcg cggaacggga agaacgcgtc agaagccatc
    29521 gaggaacctt tcacttccag gccttcatcg gcagctttaa taccggcgat tttcgcggag
    29581 tacacgcggc tcatctggcc cgcgccaata ccgatggtca tattgttttt ggcatagacg
    29641 atagcattgg atttcacgaa cttcgccact ttccagcaga acagcgcatc acgcagttcc
    29701 tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt ccgcaccgac catccccagg
    29761 tcacgatcct gaaccagcag accgccgttc acgcgtttga aatcaagacc cggaacacgc
    29821 tcgccccact gaccgcaggt cagaacgcgc acgttctgtt tggcggcggt gattttcagg
    29881 gcttcttcgc tggcggaagg cgcaataatc acttcgacaa actgacgaga aatgatggcc
    29941 tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga tgccgccgaa tgcggaggtt
    30001 gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg aattgccgat agccacgccg
    30061 caagggttgg cgtgcttcac aatcacacat gccggctcgg cgaactcttt cacgcactcc
    30121 agcgccgcat cggtatcggc gatgttgtta taagagaggg ctttaccctg aacctgggtt
    30181 gcggtagcaa cggaggcttc tttcacattc tcttctatat agaaggcagc ctgctggtgg
    30241 ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag cgtgcgtggg
    30301 aagcgaccgg cggcttcttt gctttcaccg tggtaagccg gaaccatgct gccgaagtag
    30361 ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg ctttgatggc gaggtcgaaa
    30421 cgggttgcaa gcgtcagcga tccttcgttg tcatccatct ctttaataat ggcgtcgtag
    30481 tcgctgctct tcaccacgat tgcgacatct ttatggttct tggcggcgga gcgcaccatc
    30541 gttgggccgc cgatatcgat gttctcaacc gcatcttcca gcgagcaacc ttcacgggcc
    30601 acggtctggg cgaacggata caggttaaca accaccatat cgataggctg gatctgatgt
    30661 tcttccataa tggcatcgtc ctggccacga cggcccagaa tgccaccatg tactttcgga
    30721 tgcagggtct tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc ggaaacttcg
    30781 gttaccggca gacctttttc tgctaacaga cgggcagtgc cccctgtaga cagcagctcc
    30841 acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc agaaacactg
    30901 agcagagcgc ggcggactgg acgacgttgt tgcatggtaa atcccctgga tttgactatt
    30961 acagagagcg ttagctgaat ttttcgcgaa aaactcagct aacgccccta acggggcatc
    31021 cttatttttc gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt
    31081 tcaatggtga tcacaatttt gacagtggtt accgtgggaa aaatacagaa attacattga
    31141 tgattgtgga taactctgtg cgtaaaaagg tataaagcgg gcttttgctg gggaatgcag
    31201 cagtcagtca tttttctgca atttttctat tgcggcctgc ggagaactcc ctataatgcg
    31261 cctccatcga cacggcggat gtgaatcact tcacacaaac agccggttcg gttgaagaga
    31321 aaaatcctga aattcagggt tgactctgaa agaggaaagc gtaatatacg ccacctcgcg
    31381 acagtgcgct aaagcgcgtc gcaactgctc tttaacaatt tatcagacaa tctgtgtggg
    31441 cactcgaaga tacggattct t
//
LOCUS       DAZFZC010000056        31460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFZC010000056 DAZFZC010000000
VERSION     DAZFZC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.056"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            91..432
                     /gene="yiaB"
                     /locus_tag="ACHKXP_002886"
     CDS             91..432
                     /gene="yiaB"
                     /locus_tag="ACHKXP_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO7647858.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFTHICQLVALITIGLLFIGVLNAPINTYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            478..915
                     /gene="yiaA"
                     /locus_tag="ACHKXP_002887"
     CDS             478..915
                     /gene="yiaA"
                     /locus_tag="ACHKXP_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO7647859.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(957..1952)
                     /gene="wecH"
                     /locus_tag="ACHKXP_002888"
     CDS             complement(957..1952)
                     /gene="wecH"
                     /locus_tag="ACHKXP_002888"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO7647860.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2127..2426
                     /gene="ysaB"
                     /locus_tag="ACHKXP_002889"
     CDS             2127..2426
                     /gene="ysaB"
                     /locus_tag="ACHKXP_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO7647861.1"
                     /translation="MMMNAFFPAMALMVLVGCSIPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2521..3432
                     /gene="glyQ"
                     /locus_tag="ACHKXP_002890"
     CDS             2521..3432
                     /gene="glyQ"
                     /locus_tag="ACHKXP_002890"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO7647862.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3442..5511
                     /gene="glyS"
                     /locus_tag="ACHKXP_002891"
     CDS             3442..5511
                     /gene="glyS"
                     /locus_tag="ACHKXP_002891"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO7647863.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFTEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(5790..7159)
                     /locus_tag="ACHKXP_002892"
     CDS             complement(join(5790..6679,6679..7159))
                     /locus_tag="ACHKXP_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HFO7647864.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     HISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            7239..7391
                     /gene="hokA"
                     /locus_tag="ACHKXP_002893"
     CDS             7239..7391
                     /gene="hokA"
                     /locus_tag="ACHKXP_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO7647865.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(7368..7439)
                     /gene="ysaE"
                     /locus_tag="ACHKXP_002894"
     CDS             complement(7368..7439)
                     /gene="ysaE"
                     /locus_tag="ACHKXP_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFO7647866.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFAG"
     gene            complement(7578..7790)
                     /gene="cspA"
                     /locus_tag="ACHKXP_002895"
     CDS             complement(7578..7790)
                     /gene="cspA"
                     /locus_tag="ACHKXP_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO7647867.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(8071..8361)
                     /gene="yiaG"
                     /locus_tag="ACHKXP_002896"
     CDS             complement(8071..8361)
                     /gene="yiaG"
                     /locus_tag="ACHKXP_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO7647868.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            8795..9505
                     /gene="yiaF"
                     /locus_tag="ACHKXP_002897"
     CDS             8795..9505
                     /gene="yiaF"
                     /locus_tag="ACHKXP_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO7647869.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(9555..10529)
                     /gene="ghrB"
                     /locus_tag="ACHKXP_002898"
     CDS             complement(9555..10529)
                     /gene="ghrB"
                     /locus_tag="ACHKXP_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO7647870.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVNAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(10633..11292)
                     /gene="yiaD"
                     /locus_tag="ACHKXP_002899"
     CDS             complement(10633..11292)
                     /gene="yiaD"
                     /locus_tag="ACHKXP_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO7647871.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            11445..13778
                     /gene="bisC"
                     /locus_tag="ACHKXP_002900"
     CDS             11445..13778
                     /gene="bisC"
                     /locus_tag="ACHKXP_002900"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO7647872.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(13747..14187)
                     /gene="yiaC"
                     /locus_tag="ACHKXP_002901"
     CDS             complement(13747..14187)
                     /gene="yiaC"
                     /locus_tag="ACHKXP_002901"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7647873.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(14184..14747)
                     /gene="tag"
                     /locus_tag="ACHKXP_002902"
     CDS             complement(14184..14747)
                     /gene="tag"
                     /locus_tag="ACHKXP_002902"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO7647874.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            14905..15603
                     /gene="yhjY"
                     /locus_tag="ACHKXP_002903"
     CDS             14905..15603
                     /gene="yhjY"
                     /locus_tag="ACHKXP_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO7647875.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            15832..17040
                     /gene="yhjX"
                     /locus_tag="ACHKXP_002904"
     CDS             15832..17040
                     /gene="yhjX"
                     /locus_tag="ACHKXP_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7647876.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            17364..19055
                     /gene="eptB"
                     /locus_tag="ACHKXP_002905"
     CDS             17364..19055
                     /gene="eptB"
                     /locus_tag="ACHKXP_002905"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO7647877.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            19147..19223
                     /locus_tag="ACHKXP_002906"
     tRNA            19147..19223
                     /locus_tag="ACHKXP_002906"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19181..19183,aa:Pro,seq:cgg)
     gene            19258..19391
                     /locus_tag="ACHKXP_002907"
     ncRNA           19258..19391
                     /ncRNA_class="other"
                     /locus_tag="ACHKXP_002907"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            20134..21741
                     /gene="dppA"
                     /locus_tag="ACHKXP_002908"
     CDS             20134..21741
                     /gene="dppA"
                     /locus_tag="ACHKXP_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO7647878.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(21775..21885)
                     /locus_tag="ACHKXP_002909"
                     /pseudo
     CDS             complement(21775..21885)
                     /locus_tag="ACHKXP_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22049..23068
                     /gene="dppB"
                     /locus_tag="ACHKXP_002910"
     CDS             22049..23068
                     /gene="dppB"
                     /locus_tag="ACHKXP_002910"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO7647879.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVYMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            23078..23980
                     /gene="dppC"
                     /locus_tag="ACHKXP_002911"
     CDS             23078..23980
                     /gene="dppC"
                     /locus_tag="ACHKXP_002911"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO7647880.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            23991..24974
                     /gene="dppD"
                     /locus_tag="ACHKXP_002912"
     CDS             23991..24974
                     /gene="dppD"
                     /locus_tag="ACHKXP_002912"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO7647881.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            24971..25975
                     /gene="dppF"
                     /locus_tag="ACHKXP_002913"
     CDS             24971..25975
                     /gene="dppF"
                     /locus_tag="ACHKXP_002913"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO7647882.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(26005..27276)
                     /gene="yhjV"
                     /locus_tag="ACHKXP_002914"
     CDS             complement(26005..27276)
                     /gene="yhjV"
                     /locus_tag="ACHKXP_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO7647883.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            27752..27859
                     /gene="ldrD"
                     /locus_tag="ACHKXP_002915"
     CDS             27752..27859
                     /gene="ldrD"
                     /locus_tag="ACHKXP_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFO7647884.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(27946..29625)
                     /gene="bcsG"
                     /locus_tag="ACHKXP_002916"
     CDS             complement(27946..29625)
                     /gene="bcsG"
                     /locus_tag="ACHKXP_002916"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO7647885.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(29622..29813)
                     /gene="bcsF"
                     /locus_tag="ACHKXP_002917"
     CDS             complement(29622..29813)
                     /gene="bcsF"
                     /locus_tag="ACHKXP_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO7647886.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(29810..31381)
                     /gene="bcsE"
                     /locus_tag="ACHKXP_002918"
     CDS             complement(29810..31381)
                     /gene="bcsE"
                     /locus_tag="ACHKXP_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO7647887.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPDRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         7716 a         7760 c         8117 g         7867 t
ORIGIN      
        1 ttattctgaa aatcatttgt taatggcatt tttcagtttt gtctttcgtt ggttactcgt
       61 aatgtatcgc tggtagatat ggagatcgtt atgaaaacct caaagactgt ggcaaaacta
      121 ttatttgttg tcggggcgct ggtttatctg gttgggctat ggatctcatg cccattgtta
      181 agtggaaaag gctattttct tggcgtgtta atgacagcaa cttttggcaa ctatgcatat
      241 cttcgcgcag aaaaactcgg gcaactggat gattttttta cccatatctg ccagttagtt
      301 gcgttaatca ctatcggtct cttgtttatc ggtgttttaa acgcacctat caatacttat
      361 gaaatggtga tctatcccat cgcctttttt gtctgcttgt ttggtcaaat gcgtttgttt
      421 cgctcggcat gagcaacata aagctcttac atattcagga atgaaaggaa tactgtgatg
      481 gacaacaaaa tatcaaccta ttcaccggcc tttagtattg tgtcatggat agctctcgtt
      541 ggtggtatcg ttacctatct gttagggcta tggaatgcag agatgcagtt aaatgaaaaa
      601 ggatattatt ttgccgtact ggtattagga ctgttttctg cggcgtctta tcaaaagacc
      661 gttcgggaca agtatgaagg cataccgacc acttccattt attatatgac ctgcctgact
      721 gtctttatta tctctgttgc attactgatg gtaggtctgt ggaatgcgac attattactc
      781 agcgaaaaag gtttttatgg actggctttc ttcttaagct tgtttggtgc agtagcggtg
      841 cagaaaaata ttcgtgatgc cggaataaac ccaccaaaag aaacacaggt tacccaggaa
      901 gaatacagcg aataactcac gtaagcccgg tcagtccaat gtgaccgggc ttttacttaa
      961 ctcactaatc tgtttctgtc gattcgttgt accagcatag aaagtaacaa actcgctgcc
     1021 aacgtcgcgc aaaagatcca aataatatcc agtattggcc aatttttaag ctcaattccc
     1081 cgggtgcgca gcgcatggat aatcaaggcg tggaatccgt atatacccaa tgaatggcgg
     1141 gagattaagc caagtccgcg aatggtacgc gtatccagcg tgtttttaac cagagtcaat
     1201 agcgcgattg cgcagataaa aaccatcggc ccacagtaaa gataccaggt atcggcaaaa
     1261 tttccgcgcc actgcaattc atataatgtc ccgcgagaga taataaaaac ccccgtcgca
     1321 aacagcgcgg cgctcaccca cgacagtgct ttatgctgtg tgtccatcat ccctatagcg
     1381 cggcccaaca tgccatacag aatgtagtaa aaagtatcgc cattgatata taagttaatt
     1441 ggcagccatt caaaaccgtc aattttctgc ggcactgtgt ttgggttagc gataatgcca
     1501 atcaccgcca ttagcaccag caacattttt ccgccgacgt tcttcacctg aatcagcggt
     1561 gaaaccagat aaatcaccgc aatcgcgaag aaaaaccaca agtggtaaaa cactggcttt
     1621 tgcagcaggt ttttcagcgc taactccata ttgatggagg taaacagcgc aatgtagagc
     1681 agtgcgattg cgctataaaa aatcagacat aagccgatac gcaagaaatg gcgcggctgg
     1741 gcgctgcgtt cgccaaaaaa gagatagccg gaaatcatga aaaatagcgg cacgctgaca
     1801 cgagaggcag aattcagaac attggcgata tcccatgtga cggggctaac actatgagca
     1861 ttggtcacat accaggtagt ggtgtgaatc atcaccacca ttaaacacgc tatccctcgc
     1921 aggttatcaa tccagtaaat tttgggctgc atctgtgtct ctgtatctgg ttaaaaaaag
     1981 tctgaccgat aaatcatttg gttggcgcac tggaataatc tgagttttat cactacagct
     2041 tatagaggct taaggaaatt cgtaagatat cagccactat accgatataa ataataagac
     2101 tcacctgcaa accagacggt aatttaatga tgatgaacgc tttctttccg gcaatggcgc
     2161 ttatggtgct agtgggttgt tctataccgt cacccgtgca gaaagcacaa cgggtaaagg
     2221 ttgatcctct gcgttcgttg aatatggaag cgttatgcaa ggatcaggcg gcaaaacgtt
     2281 ataacaccgg cgagcaaaaa atcgacgtca ccgccttcga acagttccag ggaagctatg
     2341 aaatgcgcgg ttataccttc cgtaaagagc agtttgtctg ttcttttgac gcggatggcc
     2401 attttttgca tctttccatg cgttaagccc tgctttttcc cgtttcgtac tgtatatctt
     2461 ccatccagcg ggtatactga tcccttcctt taaatccaca cgtatccagc acgaaataat
     2521 atgcaaaagt ttgataccag gaccttccag ggcttgatcc tgaccttaca ggattactgg
     2581 gctcgccagg gctgcaccat tgttcaacca ttggacatgg aagtcggcgc gggaacctct
     2641 cacccaatga cctgtctgcg cgcgctgggg ccagaaccga tggcggctgc ttatgttcag
     2701 ccttctcgtc gcccgaccga tggtcgctac ggcgaaaacc ccaaccgttt acagcactac
     2761 tatcagttcc aggtggtcat taagccatcg ccggacaata ttcaggagct gtacctcggt
     2821 tctctgaaag agctgggcat ggacccgact attcacgaca tccgtttcgt ggaagataac
     2881 tgggaaaacc cgacgctggg tgcctgggga ctgggctggg aagtgtggct gaacggcatg
     2941 gaagtgacgc agttcactta cttccagcag gttggtggtc tggagtgtaa accggttacc
     3001 ggcgagatca cctacggtct ggaacgtctg gccatgtaca ttcagggcgt agacagcgtt
     3061 tacgacctgg tctggagcga cggcccgctg ggtaaaacca cctacggcga cgtgttccat
     3121 cagaacgaag tggagcagtc cacttacaac ttcgaatacg cggatgtgga cttcctgttc
     3181 acctgcttcg agcagtacga gaaagaagcg cagcagctgc tggcgctgga aaatccgctg
     3241 ccgctgccag cctacgagcg tattctgaaa gccgcccaca gcttcaacct gctggatgcg
     3301 cgtaaagcca tctccgtcac cgagcgtcag cgctacattc tgcgcattcg caccctgacc
     3361 aaagcagtgg cagaagcata ctacgcttcc cgtgaagccc tcggcttccc gatgtgcaac
     3421 aaagataagt aagaggcggc tatgtctgag aaaacttttc tggtggaaat cggcactgaa
     3481 gagctgccac caaaagcact gcgcagcctg gctgagtcct ttgctgcgaa ctttactgcg
     3541 gagctggata acgctggcct cgcacacggc accgttcaat ggtttgctgc tccgcgtcgt
     3601 ctggcgctga aagtagctaa cctggcggaa gcgcaaccgg atcgtgaaat cgaaaaacgc
     3661 ggcccggcga ttgcccaggc gttcgacgct gaaggcaaac cgagcaaagc ggcagaaggt
     3721 tgggcgcgtg gttgcggtat taccgttgac caggctgagc gtctgactac cgataaaggc
     3781 gaatggctgc tgtatcgcgc ccatgtgaag ggcgaaagca ccgaagcact gctgccgaat
     3841 atggttgcga cttctctggc gaaactgccg atcccgaaac tgatgcgttg gggcgcaagc
     3901 gacgtgcact tcgtgcgtcc ggtgcacacc gtgaccctgc tgctgggcga caaagtcatt
     3961 ccggcaacca ttctgggcat tcagtccgat cgcgtgattc gcggccaccg ctttatgggc
     4021 gagccggaat tcaccatcga taacgccgat cagtatccgg aaattctgcg tgagcgtggg
     4081 aaagtcatcg ccgattacga agaacgtaag gcgaagatta aagccgatgc cgaagaagca
     4141 gcgcgtaaga ttggcggtaa cgctgactta agcgaaagcc tgctggaaga agtggcttcg
     4201 ctggtggagt ggccggtcgt tctgaccgca aaattcgaag agaaattcct cgcggtgccg
     4261 gctgaagcgc tggtttacac catgaaaggt gaccagaaat acttcccggt gtatgcgaac
     4321 gacggcaaac tgctgccgaa ctttatcttc gttgccaaca tcgaatcgaa agatccgcag
     4381 cagattatct ccggtaacga gaaagtcgtt cgtccgcgtc tggcggatgc cgagttcttc
     4441 ttcaacaccg accgtaaaaa acgtcttgaa gataacctgc cgcgcctgca aaccgtgttg
     4501 ttccagcaac agttggggac gctgcgcgac aaaactgacc gcatccaggc gctggctggc
     4561 tggattgctg aacagattgg cgctgacgtt aaccacgcta cccgtgcggg tctgctgtct
     4621 aagtgcgacc tgatgaccaa catggtcttc gagttcaccg acacccaggg cgttatgggg
     4681 atgcactatg cgcgtcacga tggcgaagcg gaagatgtcg cggtggcgct gaatgagcag
     4741 tatcagccgc gttttgctgg tgatgacctg ccgtccaacc cagtagcttg tgcgctggcg
     4801 attgctgaca agatggatac cctggcgggt atcttcggta tcggtcagca tccgaaaggc
     4861 gacaaagacc cgtttgcgct gcgtcgtgcc gcgcttggcg tgctgcgaat tatcgttgag
     4921 aagaacctca accttgatct gcaaacgctg accgaagaag cggtgcgtct gtatggcgat
     4981 aagctgacta atgccaacgt agttgatgat gttatcgact ttatgctcgg tcgcttccgc
     5041 gcctggtatc aggacgaagg ttataccgtt gacaccatcc aggcggtact ggcgcgtcgt
     5101 ccgactcgtc cggctgattt cgatgcccgt atgaaagcgg tatcgcattt ccgtaccctg
     5161 gatgcagctg ctgcactggc ggcggcgaac aaacgtgtat ctaacattct ggcgaaatct
     5221 gacgaagtgc tgagcgaccg cgtgaatgcc tctaccctga aagagccgga agaaattaaa
     5281 ctggcgatgc aggttgtggt gctacgtgac aagctggagc cgtactttac ggaaggtcgt
     5341 taccaggatg cgctggtcga actggctgag ctgcgtgaac cggttgatgc tttcttcgat
     5401 aaagtgatgg tcatggttga tgacaaagaa ttgcgtatca accgtctgac catgctggag
     5461 aaactgcgcg aactgttcct gcgcgttgcg gatatttcgc tgttgcaata ataacgccgt
     5521 tattaaatag cctgccatct ggcaggcttt ttttatcgct aaataataca gcaaccttta
     5581 ataatcttct gctgaataaa gattatctca tatattaatt ttatgagatt tttttaggat
     5641 tatatcaagg agaagaaaca aacttattaa gctagaatag ccacgggtgc ttgagactgt
     5701 ttgtctcagg tattcaccga aaggcagaca gagaaaagcc ccacctgact ataaatcaaa
     5761 gtttgtactg accccaaaaa gttggacagt taaacacgag gcatataggt ctgattccga
     5821 tattcaattg gagtcagacc ttttaatttc aggctaattc ttctgctgtt gtagtattca
     5881 atatattccg taacagcatc cttcagttcg cttatattac tgaactcatc aagataaaaa
     5941 cactccgact ttaaggttcc aaagaaacac tccaccacag cattatccag acaattgcct
     6001 tttctggaca tgctttgttt aataccatgt tctttaagga tattttgata tcttctcata
     6061 cgatactgcc atccctggtc agagtgcaga acaggatgct cgtgaggatt aagctttttg
     6121 aatgcctgat cgagcatatt ctcaaccatg ttcatcactg gtctttccga aaggctgtaa
     6181 gaaataactt cgttgttgaa gagatctatt actggagaca aatacagctt gcgcccattg
     6241 actgcaaatt cagtaacatc ggtaacccac ttctcgtttg gccgcgtagc cttgaaatct
     6301 ctttggagaa cattaggggc ggtttgccct acctctcctc tgtaagagcg gtatcgcttg
     6361 accttaatcg ctgctttaag tgagagggtt cccatcaggc gctgaacagc tttatggtta
     6421 atctgtttcc cttctcgatg aagagacagc gttaccctac ggtatccgta tcggcctcta
     6481 ttctcgtgat aaatctcact aatatgcttt ttaacgtccg catacttgtc aggcttgctg
     6541 agagccttta gatgataata aaacgtactg cgcggtatct ccgcagccct gagaagctca
     6601 tcaagaggat aaaactgcct tagctcgttg agtactttca ctttttcgtg ggatgaacta
     6661 aggctttcag cttttttaga tacataagcc gcgtttcaag aaatcgaact tgcctttcaa
     6721 gatcctcaat gcgtcggtct tttgacagct ccaatgctga tgccgctttt tctggatcaa
     6781 ctgatattgc aatgtttctt ttggtgccaa tcttgagcgc gcgtaaacca gcttctccgc
     6841 gctcttcata gaccttcagc cacctggcta cagaaccact accagcaagc ataaagtgag
     6901 cagcagcctg attaagggac atgtgctgct cgatcacagc tttcacgacc ttaatacgca
     6961 actctggatc agcactaacg cctttaggtt tgggaattaa acctttttct ccatgttttt
     7021 catagagggc aacccatgtc ctgacctggg ttcgggggac accaaaacgt gccgagatga
     7081 tcctgtaacc atcatcagtt gtgaagtagt gattcacgac ttcaaggcgc ttttcaaaag
     7141 ggtattttgg ctttgacata ttaggggcta ttccatttca tcgtccaaca aaatgggtgc
     7201 agtacatact cgttggaaat caacacagga ggctgggaat gccgcagaaa tatagattac
     7261 tttctttaat agtgatttgt ttcacgcttt tatttttcac ctggatgata agagattcac
     7321 tgtgtgaatt gcatattaaa caggagagtt atgagctggc ggcgttttta gcctgcaaat
     7381 tgaaagagta agagtcttcg gcgggaaatt attcccgcct tacttacggc gttgcgcatt
     7441 ctcattgcac ccaaatttat tcttcacaaa aataataata gattttatta cgcgatcgat
     7501 tatttatttc ctgaaaacaa ataaaaaaat ccccgccaaa tggcagggat cttagattct
     7561 gtgcttttaa gcagagatta caggctggtt acgttaccag ctgccgggcc tttagcgccg
     7621 ctttcgatgg tgaaggacac tttctgacct tcgtccagag atttgtaacc atcgttctgg
     7681 atagcagaga agtgtacgaa cacatcttta gagccatcgt caggagtgat gaagccgaag
     7741 cctttgtcag cgttgaacca ttttacgata ccagtcattt taccggacat agtgtattac
     7801 ctttaataat taagtgtgcc tttcggcgat atggcgtgct ttacagattt tgaagcgtta
     7861 aaggaatgtg cactacgagg ggtatcaacg ataactcttg aagggacttg ccttactaca
     7921 ctgctttaat ggtctgtacg tcaaaccgtt gatgtgcatt aagccacgca ttggcgggtg
     7981 atgcaacaat tatttttcat atttatgatt aatcggttgg aaaacggtcc tgtcatcagg
     8041 accgtaaaca gcaataaagt ggataaaagt ctattccatc aactgcttac ttaatgccgg
     8101 gttggcttga atcaaacgca tcaattttag ttcggcactt gaaggcttca cgcgtctgga
     8161 ttcccattcc tttaccatgg cgactgatac gcccaaaacc cgggcgaaat catcgatttt
     8221 taatcctgtc ccttttcgta actgctcaat ttcggtacag gacgttgttc tgtgcgtcag
     8281 ggtaattttc tgcgtttcat ctttaaaaac aatctgttcc aggctgctca acagctcatg
     8341 cattggatct ttatattcca ttgaaaactc ctcaaatcac actgcgggat cgtgaattgc
     8401 atcgaagctc attaagaata gtcgggaaaa gcagacacag cggggtgaag tcagggcatg
     8461 ctctggctat gattcgattc agcctgtcat ttagcgtgac ttatagccac ctaacgatct
     8521 gataaatgcc gatatatctc ttaccactcc tgttttgtaa agatttcgaa aatctaacct
     8581 tctgcgaagg cgtaaatctc tggctacaaa aaaggcaacg gcactatcat tttttaacaa
     8641 tgaaacaaca aattaacctt gtttcatttt ctccatgatt ttaccaggaa gattacgtag
     8701 aaagggtatc ctgcaagcct gtcctggact atccttgtca cgtcagacac gcgtgtgtcg
     8761 ttgtgcgctt tttttgggtg aaaggagtaa gaaaatggcg acaggaaagt cctgctctcg
     8821 ctggtttgcg cctcttgcgg cgttattaat ggtagttagc ctgagtgggt gttttgataa
     8881 agaaggcgat cagcgtaaag cgtttatcga cttcctgcag aatacagtga tgcgtagcgg
     8941 tgaacgtcta ccaaccctga ctgccgatca gaaaaaacag tttggtcctt ttgtctctga
     9001 ttacgcgatt ctgtatggtt attctcagca ggtgaatcag gcgatggatt ccggtctgcg
     9061 cccggttgta gacagcgtta acgcaattcg cgtaccgcag gattacgtta cgcaaagcgg
     9121 tccgctgcgt gaaatgaatg gttcgctggg tgtgctggcg caacaactgc aaaatgcgaa
     9181 gttgcaggcc gatgctgcgc actctgcgct aaaacagagt gatgacctga aaccggtctt
     9241 tgatcaggca ttcaccaaag tggtaacgac gccagctgat gcattgcaac cgttgattcc
     9301 ggcggcacaa actttcacgc aacaactggt gatggttgga gactacattg cccaacaagg
     9361 tactcaggtg agctttgtag caaatggcat ccagttcccg acttcacagc aggcaagtga
     9421 atataacaaa ctgattgcgc cattaccggc acaacatcag gcgtttaatc aggcctggac
     9481 tacagcagtt actgcaaccc aataaagagt aaaagcccga gcgattatcg ggctttactc
     9541 cacgcagtcg cggcttagtc cgcgacgtgc ggattcacac agttcttctc aacctttcct
     9601 tgtaacgcat caatcaaatt atccacggca caggcggcca tgccataacg cgtctcatgg
     9661 gtggcagatc caatatgcgg tactgcgacg acgttggcca ttgagagcaa cggcgaatct
     9721 acggaaagtg gctcttgttc gaagacatcc agcccggcgg cgtgaatttc gcctttctgc
     9781 aatgcagcga tcagtgcatt ttcatcaacc accgggccac gtccggcatt aatgaatatg
     9841 gcggaggatt tcattttggc gaattgttct gcgccaaata gatgatacgt ctcatcagtt
     9901 aacggcagga tcaggcaaac gaaatctgac tcttgtaaca gtgtatccaa atcgcagtag
     9961 cgggcgttga agcgttcttc tgcttcttta tggtggcggc gcgcgttata gaggatgggc
    10021 atgttgaagc caaagtgcgc acgttgtgcc agcgccatac cgatccgtcc catcccgaca
    10081 atgcccagtg ttttatggtg aacgtcagtg ccgtaccagt ccgggcctat gctcgcggtc
    10141 cattcgcctg cttttacccg ttctgctacc tccacaaccc gacgagcggt agacaacacc
    10201 agcgccatca gcgtatcggc gacggtttct gttaatacgg ttggcgtgtg catcagcaga
    10261 atttttcggg cggtaagcgc atcgacatca aaattgtcat agccgacgga gatcgttgat
    10321 gtggcacgca gtttcggcat tttttccagc aatgcggcat ttacattctc gtttgaaccc
    10381 agtaaacctt cagcttctgc aaaaattgct gcattttgat cgacggtttg tgggctgagg
    10441 tttgccacct ggtgaacggt gaaatgctct tgcaggcgtt gcagtaaatc atcaggtaag
    10501 gctttgtaga ggataacgga cggcttcatg cttctctcca ttcactgata aggcgatcac
    10561 cttagcctga aatatgatga aggaaaactg aagaatgcca ggtgatttct gcatcacctg
    10621 gcatgaaagg gattacagcg ggcttaaggt aatttctaca cggcggtttt gcgccttacc
    10681 ttctgcggtg ctgttgctgg cgattgggtt agccgggcca aggccctgag tacggatgcg
    10741 gctggcgtcc acgccctggg tgatcaacgc gctggcaacg gaatccgcac gttgctggga
    10801 gagacgcatg ttcaggtcgt gaccacccgt gctgtcggta taaccaatca cgttaaccgc
    10861 cgttttcgga tactctttca gtaccattgc cacgccggtc agggtgttag cgcccgccgg
    10921 tttcagggtc gcgctgctgc tgtcgaaggt cacattgttc ggcatattga ggataatgtt
    10981 atccccgctg cgggttacgc taacaccagt gccgcgcatt ttgtcgcgca gcttcgcttc
    11041 ctgcacatcc atgtaataac caacgccgcc gcccagagct gcgcctgctg ctgcgccaat
    11101 cagcgcgcct ttaccgcgat ctttcttcga agaagagagc gcaccaatac ccgcgcccac
    11161 gagagagccc agacctgcgc cgatagcaga tttacctgct tcgcgttcgc cggtgtaagg
    11221 gttagttgtg cagccagata ccgccagagc gccactcact acggcggcaa taagataaac
    11281 acgtttcttc attgttaatc cttaataacc tttttattct ttgccacggg ttccgtggcg
    11341 ggagattatg ccgcgtgaac atgaagatta ttcctgggaa tactcggaaa tttgtaagta
    11401 atatttaact gctcaataca tctaaccttt caggagtctt cggtttggcc aactcatcct
    11461 cacgatattc cgttctgact gccgcccact gggggcccat gctggttgaa accgacggcg
    11521 aaaccgtgtt tagctcgcgt ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg
    11581 cggttcgcga ccaggttcac agcaatacgc gggtacgatt tccaatggtg cgcaaaggct
    11641 ttctcgcgtc accggaaaac ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga
    11701 gttgggatga ggcgctggat cttattcacc aacaacataa acgcattcgt gaggcttatg
    11761 gtccggcatc gatttttgct ggttcctacg gctggcgttc aaacggcgtg ctgcataagg
    11821 cctcgacatt attacaacgc tatatggcgc tggcaggcgg ttataccggg catctggggg
    11881 attattcgac tggtgcggca caggcgatca tgccgtatgt cgtaggtggc agcgaagttt
    11941 atcaacagca aaccagttgg ccgctggtgc tggaacatag cgatgtcgtg gtgctgtgga
    12001 gtgctaatcc actcaatacg ctgaaaattg cgtggaatgc atccgatgag caggggcttt
    12061 cttacttttc tgcactgcgt gacagtggga aaaagctgat ctgtattgat ccaatgcgat
    12121 cggaaaccgt cgatttcttt ggcgataaaa tggagtgggt ggcaccgcac atgggcaccg
    12181 atgttgcgct gatgctgggg atcgcccata cgctggtgga aaatggttgg cacgacgaag
    12241 cgtttctggc gcgttgcacc acaggttatg ccgtcttcgc ctcttatttg ctgggcgaga
    12301 gtgacggaat agcgaaaaac gccgaatggg cagcagagat ttgtggtgtt ggcgcagcga
    12361 aaatccgcga gctggcggct attttccacc aaaataccac catgctgatg gcaggctggg
    12421 gaatgcagcg ccaacagttt ggtgagcaaa aacactggat gatcgtcacg ctggcagcaa
    12481 tgttggggca aatcggcaca cccggcggcg gttttggtct ttcttaccat tttgccaatg
    12541 gtggtaaccc cacgcggcgt tctgcggtgc tctcttccat gcagggcagc ttgccgggtg
    12601 gctgcgatgc ggtggataaa atccctgttg cccgcattgt tgaagcactg gaaaaccctg
    12661 gtggcgcata tcaacacaac ggtatgaacc gacatttccc ggatattcgt tttatctggt
    12721 gggcgggcgg tgccaacttt actcatcatc aggataccaa tcgtctgatc cgtgcctggc
    12781 aaaaaccgga gctggtggtg atctctgaat gcttctggac ggcggcggcg aaacacgcgg
    12841 atatcgttct gcctgcgact acctcttttg agcgtaatga tctcaccatg accggtgatt
    12901 acagtaatca gcatctggtg cccatgaagc aagtggtgcc gccacgctat gaagcgcgta
    12961 atgattttga tgtttttgcc gagttgagtg aacgttggga gaagggcggc tatgcgcggt
    13021 ttacggaagg aaaaagtcag ctgcaatggc tggaaacgtt ttataacgtt gcccggcagc
    13081 gcggggcaag ccagcaggtt gaattgccgc catttgctga gttctggcaa gccaaccagt
    13141 taattgagat gccggaaaac ccggacagcg agcggtttat tcgcttcgct gatttttgcc
    13201 gcgatccgct ggcgcatccg ttaaaaaccg ccagcggcaa gattgaaatc ttctcacagc
    13261 gtattgccga ttacggttac ccggattgcc ctgggcatcc aatgtggctg gagccggacg
    13321 aatggcaggg caatgccgaa ccagaacagt tgcaggtact ttctgcccat ccggcgcacc
    13381 gcctgcacag ccagctgaat tacagttctc tgcgcgaatt gtacgcggta gcaaaccgtg
    13441 agcctgtcac cattcatcct gacgatgctc aggcgcgcgg cattcaagat ggcgatactg
    13501 ttcggctgtg gaacgcacgc gggcaaattc ttgccggagc ggtcattagc gagggaatta
    13561 aacctggcgt gatttgtatt cacgaagggg catggccgga tttggattta accgctgacg
    13621 gtatttgtaa aaacggcgca gtcaacgtgc tgaccaaaga tctccccagc tcgcggctgg
    13681 ggaatggctg tgcgggtaat acggcgctgg catggctgga aaaatacaac ggtccggaac
    13741 tgacacttac agcgtttgaa ccaccggcca gctcataatc catgtgggta gttgggtttc
    13801 atcctgccat gcgcaatcga caatgtgaaa accctgtgcc tggtaaaaat ttatcgccgg
    13861 ttgatttttt tgataaacct ccagcatcag gtggggatgg cgctgctgca catactgcat
    13921 cagcgcctta ccaataccgc gcctgacggc cttcggtgcg acaaacatcg ctgccagaaa
    13981 tcggccttcc ataatgctga caaaaccgag aagcttaccg tcttcttccc agacccagtt
    14041 ttgcgcgttg gcaagatagg catcccgcac cagcggaatg cagtcacgcc agtaattcgc
    14101 ttttataaag ggatgccccc aggttgtact ttccagccac agttcgagga tcgcggggag
    14161 ttctgaacgt tgcgcttccc gaatcatggt ttatttcccg gatagcaaca gcagccaacc
    14221 acatgatcat tcaccagccc acatgcctgc ataaaggagt aacagattgt ggtgccgaca
    14281 aacttaaaac cacgtttttt cagtgcctta gatagggcgt cggaggcggg cgtagatgtg
    14341 gggatttcgc tcaacgttgt ggcttgtgtc acctgtggct gatgatttac aaacgaccag
    14401 acaaagtcgg caaacggttc gccgttctgt tccatttgca ggtacgcccg cgcattacca
    14461 ataattgcct gaattttccc gcgatggcgg ataatcccgg cgtcctgtac cagtctttcg
    14521 acatcctctt cctgcattgc ggcaaccttc accggatcga actgatgaaa gcaggcgcga
    14581 tagttttcgc gttttttgag gacggtgatc cacgataacc cagcctgctg cccttccagg
    14641 cagatcattt cgaacagttt tttactgtca gtttcaggca cgccccactc attatcatgg
    14701 taggcaatat aaagcgggtc ctgactcacc cagccgcaac gttccatact ttccctcgct
    14761 atgtacccga tcgtaaaaat ttcagccgct tttcttgacg catcggtgaa aaagtcaata
    14821 tttaatacag ggctactatg cccgatatcc ttcatacaat gacaataata ttgccgaatt
    14881 cggctcttcc ctggagtcgt gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta
    14941 agcctgctgg cgagtgtggt aatcagtgcc ttttttctca acacagctta cgcctggcaa
    15001 caagaatata tcgttgatac gcaacccggg cattccacag agcgttacac ttgggatagt
    15061 gatcatcaac ctgattacaa cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg
    15121 ctgggactgg aagtcaatct ggcggaagaa acccctgtgg atgtgaccag cagtatgagt
    15181 atgggctgga attttccttt atatgaacag gttacaactg gcccggtcgc ggcattacat
    15241 tacgatggca caaccacctc gatgtataac gagtttggcg acagtactac cacgctgacc
    15301 gatccgttat ggcatgccag cgtgagtacc ttaggctggc gtgttgactc ccggcttggc
    15361 gatctccgac cctgggcgca aatcagctat aaccagcaat ttggcgagaa tatctggaag
    15421 gcgcaatcag gcctgagccg gatgacggcg acaaaccaga acggcaactg gctggatgtc
    15481 accgtaggcg ctgatatgtt gctcaatcaa aatattgccg cctatgccgc gctatctcag
    15541 gcagaaaata ccactaacaa tagcgattat ctgtatacga tgggggttag tgccagattt
    15601 taacgtaaca atcacaattg aaaccattga ataacaatag ttgtggcgat agtgggtgct
    15661 aacttaccaa ataataaatt tggtgaataa ttgtcgcgtc attcattcct gaactaaggc
    15721 atttcattcc gttctgatgg catttcatgc cgtttttccc caggcataaa gtgcacttcg
    15781 ttatggttgt cggcagagat ttttcctttt tattactgca ggaatactgc catgacacct
    15841 tcaaattatc agcgtacccg ctggctgaca ctcatcggta ctatcattac ccagtttgcg
    15901 ctggggtcgg tttatacctg gagcctgttt aacggcgcgc tttccgccaa gctggatgcg
    15961 ccggtaagcc aagtcgcttt ctctttcggc ttgttaagtc tggggctggc aatttcgtct
    16021 tctgttgcgg gcaaattgca ggaacgtttt ggtgttaaac gcgtcacaat cgcttccggc
    16081 attttgctgg gattagggtt cttcctgaca gcgcattctg acaacctgat gatgctgtgg
    16141 ttaagcgccg gtgtgctggt cggtctggcg gatggcgcgg gttacctgct gacgctctct
    16201 aactgcgtga aatggttccc ggagcgtaaa ggtctgatct ccgcgttcgc tatcggttct
    16261 tatggtctgg gtagcctggg tttcaaattt atcgacacgc agctgctgga aacggtcggt
    16321 ctggaaaaaa cctttgtgat ttggggagcg attgcgctgg tgatgattgt tttcggcgca
    16381 acgttaatga aagacgcacc aaaacaggaa gtgaaaacca gcaatggcgt agtggagaaa
    16441 gattacacgc tggcagagtc gatgcgtaaa ccgcagtact ggatgttagc ggtaatgttc
    16501 ctgactgcct gcatgagcgg cctgtatgtg attggggtag cgaaagatat cgcccaaagt
    16561 ctggcacacc ttgatgtggt ttccgcagcc aatgcagtca ctgttatttc catcgccaac
    16621 ctttcaggtc gtctggtgct gggcattctg tctgacaaaa tcgcccgtat ccgtgttatc
    16681 accattggtc aggtaatttc gcttgtgggt atggcggccc tgctgtttgc accattgaat
    16741 gcagtgacgt tctttgcagc gattgcctgc gtggcattta actttggcgg cactattacc
    16801 gtctttccgt cactggtcag tgagttcttc ggcctcaata acctggcgaa aaactacggt
    16861 gtgatttacc tcggtttcgg tatcggtagc atttgtggtt cgattatcgc ctcactgttt
    16921 ggcggcttct atgtgacgtt ctacgtcatc tttgctctgc tgattctgtc attggcgctt
    16981 tctacgacga ttcgtcagcc agagcagaaa atgttgcgtg aggcgcatgg ctccctttaa
    17041 tgctcagccg cgagctactg ggcttcagct tccgtgtgca tcaaacgcat caacgaaatg
    17101 tataaacaat gactcgtaaa atttgggcca attaactggc cctttttact gctttttgta
    17161 acgctaaagt ctcttttcaa acttgcattt ttgtaaattt gtgcttcatg cacactcttt
    17221 ccccacactt tttccctttg ctgtggtcta cttattcgcg cgtgtagatt ttacttatct
    17281 gactacctcc gcactttttc cctgccgggc ctgaaaagcc actaagcagg gtgttatcac
    17341 ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc gattacacag cagaagctga
    17401 gctttttgct tgcaatctat attggccttt ttatgaatgg cgcggttttt taccgccgct
    17461 tcggcagcta tgcgcacgat tttaccgtct ggaaaggcat ttctgctgtt gttgaactgg
    17521 ccgccaccgt actggtgacc ttctttttac tacgtcttct ttcgctgttt ggccgccgca
    17581 gctggcgtat tctggcatcg ctggtggtgc tcttttccgc aggtgccagc tattacatga
    17641 ccttccttaa tgtggtcatt ggttatggca tcatcgcttc cgtcatgacc accgatatcg
    17701 acctgtcaaa agaagttgtt ggtctgaact ttattctctg gttaatcgcc gttagtgcat
    17761 tgcctcttat ccttatctgg aataaccgct gtcgctacac cttgctccga caactgcgaa
    17821 ccccagggca gcgtattcgc agcctggcgg tcgtcgtact ggcgggtatt atggtttggg
    17881 caccgattcg tttgctggat atccagcaga agaaagtgga gagggcgacc ggcgttgatt
    17941 tgccgagtta tggcggtgtc gtagcgaact cttatctgcc atcaaactgg ctttctgcgt
    18001 tggggctgta tgcctgggcg cgggtcgatg aatcttccga taataattca ttgcttaatc
    18061 cggcgaagaa attcacttat caggcaccgc aaaacgttga tgacacttat gtcgtgttta
    18121 tcatcggtga aaccacgcgt tgggaccata tgggtatttt cggctatgag cgtaatacca
    18181 cgccgaaact ggcccaggag aaaaatctgg cggcgttccg tggttactcc tgtgataccg
    18241 caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg gggcgcggaa gataatccgc
    18301 agcgcacatt aaaagaacag aacattttcg cggttctgaa gcagttagga ttcagttctg
    18361 acctctacgc tatgcagagc gaaatgtggt tctacagcaa cacgatggcg gacaacattg
    18421 cttatcgtga gcagattggt gcggagccac gtaatcgtgg caagccggta gatgatatgt
    18481 tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc ggatggtaag catctgatca
    18541 ttctgcatac caaaggttcg cattttaact acacccagcg ttatccgcgt agcttcgcgc
    18601 agtggaagcc ggaatgtatt ggtgttgata gcggctgtac caaagcgcag atgatcaact
    18661 cctatgacaa ctcggtgacc tatgtggatc actttatctc cagcgtgatt gatcaggttc
    18721 gcgataagaa agcgattgtg ttctacgcag ctgaccacgg tgagtcaatt aatgaacgcg
    18781 agcacctgca cggcacgccg cgtgaactgg caccgccgga gcagttccgc gtaccgatga
    18841 tggtctggat gtcagataaa tatctggaaa atccggccaa tgcgcaggcg tttgcgcagc
    18901 tgaaaaaaga agccgacatg aaagtgccac gccgtcacgt agagctgtac gataccatca
    18961 tgggttgtct tggctatact tcaccggatg gtggaattaa cgaaaacaac aactggtgtc
    19021 acatcccgca ggcaaaagag gcagcggcta actaagcggc ctgctgactt tctcgccgat
    19081 caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta agatgcgccc
    19141 cgcattcggt gattggcgca gcctggtagc gcacttcgtt cgggacgaag gggtcggagg
    19201 ttcgaatcct ctatcaccga ccaaattcga aaagcctgct caacgagcag gcttttttgc
    19261 atctgcagct catgaggatg agaacctccg ggggcaggga ggttcgactc gagcgaagcg
    19321 agagaacgtt gcgccagcaa cggcccgcag ggcgagccac gaagtggcga gtaatcctct
    19381 atcaccgacc aaattcgaaa agcctgctca acgagcaggc ttttttgcat ctgcagctca
    19441 tgaggatgag aacctccggg ggcagggagg ttcgactcga cgtaggcctg ataagacgcg
    19501 tcagcgtcgc atcaggcaaa tgtgccggcc attgtcggat gcggcgcgac cgccttattc
    19561 gacctacacg acacacattt ccctgccaaa tttgcgcccc atatctcact atccattaac
    19621 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt tttgcgttac
    19681 ttatggctgg ccctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca
    19741 gatatccacc ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt
    19801 tgctaaatat taagctgatt gttctggtca tgttggagta gcacaaattt ctctgctgca
    19861 aatagctgtt tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc
    19921 cggttggctg tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt
    19981 ttatggagga tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac
    20041 gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt
    20101 taaaacaaca aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg
    20161 atgctgaagc ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa
    20221 actctggttt attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc
    20281 ggcaccacct atgacgcctc ttccgtcccg ctttataacc gtctggttga atttaaaatc
    20341 ggcaccaccg aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa
    20401 acctatacct tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg
    20461 acgcgtgaac tgaacgccga tgatgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa
    20521 aacccgtacc ataaagtttc tggcggcagc tacgaatact tcgaaggcat gggcttgcca
    20581 gagctgatca gtgaagtgaa aaaggtggac gacaacaccg ttcagtttgt gctgactcgc
    20641 ccggaagcgc cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa
    20701 tatgctgatg cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga
    20761 accggtccgt tccagttaca gcagtatcaa aaagattccc gtatccgcta caaagcgttt
    20821 gatggctact ggggcaccaa accgcagatc gatacgctgg ttttctctat tacccctgac
    20881 gcttccgtgc gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac
    20941 ccggcagata tcgctcgcat gaagcaggat aaatccatca atctgatgga aatgccgggg
    21001 ctgaacgtcg gttatctctc gtataacgtg cagaaaaaac cactcgatga cgtgaaagtt
    21061 cgccaggctc tgacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc
    21121 gcgggcgtat cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt
    21181 caggactaca cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa
    21241 aaaggtttct ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct
    21301 cgccgcatgg cggagatgat tcaggcagac tgggcgaaag tcggcgtgca ggccaaaatt
    21361 gtcacctacg aatggggtga gtacctcaag cgtgcgaaag atggcgagca ccagacggta
    21421 atgatgggct ggactggcga taacggggat ccggataact tcttcgccac cctgttcagc
    21481 tgcgccgcct ctgaacaagg ctccaactac tcaaaatggt gctacaaacc gtttgaagat
    21541 ctgattcaac cggcgcgtgc taccgacgac cacaataaac gcgttgaact gtacaaacaa
    21601 gcgcaggtgg tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt
    21661 gaaccggtac gtaaagaagt taaaggctat gtggttgatc cattaggcaa acatcacttc
    21721 gaaaacgtct ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaaatgc
    21781 ctgatgcgct ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt
    21841 ttgtaggccg gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac
    21901 aatctgtata cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc
    21961 attccctgtc cgataagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg
    22021 ctgcgggtca ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact
    22081 cgtcatcccc acgtttatcg gtattaccct tctcacattt gcctttgtct acatgatccc
    22141 gggcgatccg gtgatgatca tggcgggcga acgtgggatc tccccagagc gtcacgcgca
    22201 gctgctggct gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg
    22261 gggcgttatg catggcgatc taggcatttc aatgaaaagc cgcatcccgg tttgggaaga
    22321 gttcgtgccg cgcttccagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac
    22381 ggcagtcggt attccggtcg gcgtgctggc tgcggttaaa cgcggttcca ttttcgatca
    22441 cacagcggtt ggcctggcgc tgacaggtta ttcaatgcct atcttctggt ggggcatgat
    22501 gctgatcatg ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga
    22561 tatggtgttc ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat
    22621 ctggggtgaa gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ctgccattgt
    22681 gctgggtact attccgctgg cggtcattgt gcgtatgaca cgctcctcga tgctggaagt
    22741 gctgggcgag gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat
    22801 tatcgtccat gcgctgcgta acgcgatgct gccggtggtg accgttatcg gcctgcaggt
    22861 gggaacattg ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg
    22921 acgctggttg attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt
    22981 gctggtggcg acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt
    23041 gaacccgcgt attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa
    23101 agtgattagc gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg
    23161 caacaaaggc gcggtcgtcg ggctggttta cgtcgtcatc gtgctgttca tcgcgatctt
    23221 tgccaactgg attgcaccct ataacccggc ggaacagttc cgcgatgcac tgctcgcccc
    23281 gccagcctgg caggaaggcg gcagcatggc gcacttgctg ggcaccgatg acgtaggccg
    23341 tgatgtgctg tcgcgcctga tgtacggtgc gcgcctgtcg ctgctggttg gctgtctggt
    23401 agttgtgtta tcgctgatta tgggcgttat tctcggcctg atcgccggtt actttggcgg
    23461 cctggtcgat aacatcatta tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct
    23521 gctggcgctg gtgctggtgg caattttcgg cccgtcgatt ggtaacgccg cgctggcact
    23581 gaccttcgtt gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaagt
    23641 taaccgcgat tacgtcaccg cgtctcgcgt ggcgggtgcc ggggcgatgc gtcagatgtt
    23701 tattaacatc ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc
    23761 taacgccatt ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac
    23821 gcctgagtgg ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt
    23881 cgtgaccttc ccgggtctgg cgatcctgct gacggtgctg gcatttaacc tgatgggtga
    23941 cggtctgcgt gacgcgctcg atcccaaact gaagcagtaa gaggttcgag atggcgttat
    24001 taaatgtaga taaattatcg gtgcatttcg gcgacgaaag cgcgccgttc cgcgccgtag
    24061 accgcatcag ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct
    24121 ccggtaagtc ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa
    24181 tggcagaaaa actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc
    24241 gcaacctggt gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc
    24301 cgtgctacac cgtgggtttc cagattatgg aagcgattaa ggtgcatcag ggcggcaaca
    24361 aaagtacccg ccgtcagcga gcgattgacc tgctgaatca ggtcggtatt cccgatccgg
    24421 catcgcgtct ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga
    24481 tcgccatggc gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc
    24541 tggacgtgac cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga
    24601 acatggcgct ggtgttaatt acccatgacc tggcgctggt ggcggaagcg gcacataaaa
    24661 tcatcgtgat gtatgcaggc caggtggtgg aaaccggtga tgcgcacgcc atcttccatg
    24721 cgccgcgtca cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca
    24781 aagaacgtct ggcgtcgttg ccaggtgtcg ttcccggcaa gtacgaccgc ccgaacggct
    24841 gcctgcttaa cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc
    24901 tgaatatgct cgctgacggg cgtcagtcca aatgccatta cccacttgat gatgccggga
    24961 ggccgacact atgagtacgc aagaggccac cctgcaacaa ccgctgttgc aggctatcga
    25021 cctgaaaaaa cattatccgg tgaagaaagg catgttcgcg ccggaacgtc tggttaaagc
    25081 gctggatggc gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc
    25141 tggctgcggt aaatcgaccc tcggtcggtt gctgacgatg attgaaatgc ccaccggtgg
    25201 cgagctgtat taccaggggc aggatctgct taagcacgat ccgcaggcgc agaagctgcg
    25261 tcggcagaaa atccagatcg tcttccagaa cccttacggt tcgctgaatc cgcgtaaaaa
    25321 agtcgggcaa attcttgaag agccgctgct gatcaacacc agcttaagca aagaacagcg
    25381 tcgggaaaaa gccctgtcga tgatggcgaa agtcggcctg aaaaccgagc actatgaccg
    25441 ctatccgcat atgttctccg gcggtcagcg tcagcgtatc gccatcgccc gtggtctgat
    25501 gctcgacccg gatgtggtga ttgccgatga accggtttcc gcgctggatg tttcagtgcg
    25561 cgcgcaggtg ctgaatctga tgatggattt gcagcaggag ttggggctgt cttatgtctt
    25621 tatctcccac gacctgtcgg tggtggagca cattgctgat gaagtgatgg tgatgtacct
    25681 gggccgctgc gtggagaagg gaacgaaaga ccaaatcttc aataacccgc gccatccgta
    25741 cactcaggcg ctactttccg cgacgccgcg cctgaacccg gacgatcgcc gcgagcgcat
    25801 caagctcagc ggtgaactac caagcccact gaatccaccg ccgggttgcg ccttcaacgc
    25861 ccgctgtcgt cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg
    25921 cggtcaactg gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac
    25981 aaaaccgggg ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag
    26041 caaaatgccg tacagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc
    26101 gactttatag atcaggaaaa acggaatgag gcacgacaca ataccatata acgggcttcc
    26161 caactgaaag aacaccagca ccgatacacg aaacgaaacc caaatcgtca acgtaatgac
    26221 gataaaagcg cagatcgcca gagtcagcac gcgtgagtta attttcttgg tatcaataat
    26281 tcggcttaac agattgagaa taatgccttt aatggcctcg tggaaaccga gataaatgcc
    26341 aaagaatgcg gtcagtacgg caaagatatt aagcaccgta gaggtgatat gaatgatatg
    26401 cccagggatc acctgcgcgg ccagcgccag tgctgagata ttttgttcaa aggcagaaac
    26461 ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat
    26521 gagcgtgata taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc
    26581 cgcttcccgt ttacgatagg caatattcat tgggttaagt acctgaataa atactgcaga
    26641 aaagaagcaa aatggaatgg taagcaagac atcgcggaaa aagacggagg cttgcgggaa
    26701 ggcggttata ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat
    26761 ccctactttg accaccacca ttggcccgga aatcttaaac agtaatcgtt caccaccaga
    26821 cgcaatcgcc accagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc
    26881 ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga
    26941 gtagataaaa atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca
    27001 gttcttcccc aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga
    27061 aagggttttt aaataaatgt cctgcactac ccaggtggca ggataagcaa tgatcgctgc
    27121 ggtaataaat acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc
    27181 accaatcgcc atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa
    27241 tttctgattg tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg
    27301 gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg
    27361 cagtatgtaa aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta
    27421 caaaattgcg catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc
    27481 aaataaagaa attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag
    27541 taaaaagcaa tcgtagaatc agcggcaagc cggaaaggtt ccggtgaggc gcaatgttgc
    27601 gggggcttta tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg
    27661 tatgcacctg acaacaccac gggggctaat cttgactcta gaccactcaa gaatagccgc
    27721 gaaacgttgt cattacaaca caggcggcta tatgacgttc gcagagctgg gcatggcctt
    27781 ctggcatgat ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg
    27841 gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg
    27901 cccggatcag gccgcagagg ttaaactctg cggctttttt agtgcttact gcgggtaagg
    27961 cacccagtcg ccgccgttca ggcgaacgta cggtttatcc tggtattgaa taactactgc
    28021 atttgagttc tcggagaccg gtgctgtttg tggcaaccca ctggtgagtt ttttccagtc
    28081 aacattgtct tcggtgaaaa tcttgccatc aagaacgcga accaccagat cggagatagc
    28141 caggaagctg ctcggttggt cgatgacaat cggtgccccc tgatgtggtg ccttcatgcc
    28201 gaagaatttc accccaacgg ggacgtctgt gatagacggg ctagggatat cacgtaggcc
    28261 agatacctgc attctgtcgc ccttcagcgc gccgccgtgt tccggcacca cgaccaccat
    28321 cactttacga cccgatttct ccagttcagt aaagaaggcg tccagttcat caaagaattt
    28381 ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc ggataatggt tgccgtcatg
    28441 cagtggaagc gtgttgtaga acgtggcact acggctgttt ttatcttttt cggtaacgtc
    28501 cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc gaaccatcaa agcccagcaa
    28561 aataaccggc agatttgttt gatccatcaa ttcagtctgc atgccgccat tttcgcgaac
    28621 ttctttcaaa aaaccgccga actggccgtt atgtcccatc atcaggtgct gggtaaagcc
    28681 cagtttcgaa aggttatcaa acagatagca gtcgttattt gccggttgat acagattagt
    28741 gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc gccgccgggc cactgtagga
    28801 ggtggcggag ttaaagttct tgaactcaat atcgaaatgc gaccacagtg gatgcgacat
    28861 caacccggcg gcttctatat ccgaccagga aagcgaacag atgttaatca ccagtagttc
    28921 aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc gatttacgtt tcgcctccgc
    28981 gttatagaaa ttattcagcc aggcgttaag gttcgccgtt gttggcggtg cagtttgtgc
    29041 gggcatatca cccactaccg gtgcgccacc cgtcgccgca acggttgccg ctgcgttacc
    29101 acccgtcgtt gttacagtgg tcgtcggttg tccggctggc cacaaggaga aacttggtcc
    29161 cgccagggta agtacgttca gccatagcag tatggcaacc acaaaaacag taatgcgaat
    29221 ccattgtgac aggaataacc aggccactaa taaaacaaaa atggccccaa tcatctgcca
    29281 gttaataaag cgtgtgacaa ggtcgattaa ataatcggta ctgaaccccg ccacctgcga
    29341 accctggctc attatgcttt ccgggccagg caaccaggtg tcatgccaga acaaagcaaa
    29401 gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg ctgtagcgcg gaatgggcat
    29461 cagcagaaac gcggcaaaca ccaaattgag gagcggatgg aagttaagat atcccgccca
    29521 caacaggccg aacttaacca gaaaatagaa gttccagccg gaaaggccgc gccagtattg
    29581 ccagagggaa gaaggcatgg cggtattttg cgtaaattga gtcatttttt ggttgccctg
    29641 gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat aacgaggttt agcaaagaac
    29701 aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg ccagatagcc cagcgggaaa
    29761 aatatcagtg cgcaaacgac aataatttca atgatatcgc tgatggtcat catgatgagc
    29821 gctccacagc atcatctaac agtcgcatgg gttctggtat tcgtcgccag tggcgaccat
    29881 cgtgctcggc attgatgacc ggtttagaac tttgcgttaa aggcagcggc atgccccatt
    29941 gttctggggc aagcaagcgc atctgcacca gctcggcact gatttgatca tcttcaaacc
    30001 agaccatacg gtttgagaaa atgtcgccag taggcaatgg gaaaatatga ttcaacgcgg
    30061 tatccagatc gttaatccga cagaatgaga gaaacagcac cagccgatta ccgccaatgg
    30121 tcatgatatc gccggtacgg tcagggcgac acagcgtcag ggcttgttca acgcggatac
    30181 ccggtaccgg acgtagggca accagaacgc ctttaccgtg ggcaggtaat agagggttat
    30241 tcatcatgtt gttgacggca ttacagaaca catcccactt ctggaaacca cgcagtttga
    30301 gcggctgggt cattgacagc aaggtagtga tatcttccgg cacatagcga ctaaacttct
    30361 gcccttgcac gctttcgatc atcgtcagac aacgggagag tggcgcattc cacggaataa
    30421 ccatatttgc accgcaggcc aataacaaac gttcatcggt ggcgcgcagg ctagcggtat
    30481 tttcccgcac gaggattttc atcgcactac cgcgctggcg acgcagggta tgaatgctgc
    30541 gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa gaccaccgtc gccgcctgag
    30601 cggtacgggc ttcattgaac aggacttcgt tattgttgaa cagttgccag tgttccgata
    30661 gcggcggcgc accttccagt acagcaacat tactcagaat gcgtttttcg tcgctgcgtg
    30721 gttggatctc cgcctcttcg ctttgaacta atgtccagat accattttgt tgctgaacgc
    30781 taagctgctg acgggcgctg accccttttt cgttgcacca gaaggcaata tccagcaaat
    30841 gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag tgaacggtat tcctcaagca
    30901 acaatgaaaa ttgtttatcg ttattatttc cgggattaat taccaaaagc gaacaatggt
    30961 ttaacctgct ccatttattc attttatcca accatgagcg aagccgctcg gcaggaatgt
    31021 tttgccatgc gttatttgcg caaacaagaa taaaaaggta attatgggga tcaatagaac
    31081 actgcaaatc acgggtcaaa tagtatagac ctttttcatg atttagcatt gaaaataatt
    31141 ttattttttc cggcccttga gaatcatcta attgaaagat tttcgccgga tcgctgtcca
    31201 tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc aattgtttga ttcgccagac
    31261 tgatagcatc ttcatggcga tcgacgttaa accaccagac gccgcctgct ggcatatgtc
    31321 gcagctcatc ccataatgat gagataccga tagagaatac agggtccaca atgtccctca
    31381 tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc taagaaataa acctaccatt
    31441 gaaattaaaa cttatcagat
//
LOCUS       DAZFZC010000057        31179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFZC010000057 DAZFZC010000000
VERSION     DAZFZC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.057"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            156..3908
                     /gene="yfaL"
                     /locus_tag="ACHKXP_002919"
     CDS             156..3908
                     /gene="yfaL"
                     /locus_tag="ACHKXP_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO7647888.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPEPDPTPDPDPTPDPDPTPDPAPVPVYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVDGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYA
                     WFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4036..4758)
                     /gene="ubiG"
                     /locus_tag="ACHKXP_002920"
     CDS             complement(4036..4758)
                     /gene="ubiG"
                     /locus_tag="ACHKXP_002920"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO7647889.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4905..7532
                     /gene="gyrA"
                     /locus_tag="ACHKXP_002921"
     CDS             4905..7532
                     /gene="gyrA"
                     /locus_tag="ACHKXP_002921"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO7647890.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATAHGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7681..9369
                     /gene="yfaA"
                     /locus_tag="ACHKXP_002922"
     CDS             7681..9369
                     /gene="yfaA"
                     /locus_tag="ACHKXP_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO7647891.1"
                     /translation="MSGEKKAEGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9366..9989
                     /gene="yfaT"
                     /locus_tag="ACHKXP_002923"
     CDS             9366..9989
                     /gene="yfaT"
                     /locus_tag="ACHKXP_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO7647892.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQAGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10013..14527
                     /locus_tag="ACHKXP_002924"
     CDS             10013..14527
                     /locus_tag="ACHKXP_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO7647893.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQTKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPVQQSVAA
                     GSEWTGMQVK"
     gene            14528..16177
                     /gene="yfaQ"
                     /locus_tag="ACHKXP_002925"
     CDS             14528..16177
                     /gene="yfaQ"
                     /locus_tag="ACHKXP_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO7647894.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVNPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16182..16958
                     /gene="yfaP"
                     /locus_tag="ACHKXP_002926"
     CDS             16182..16958
                     /gene="yfaP"
                     /locus_tag="ACHKXP_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO7647895.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17032..18216)
                     /gene="atoB"
                     /locus_tag="ACHKXP_002927"
     CDS             complement(17032..18216)
                     /gene="atoB"
                     /locus_tag="ACHKXP_002927"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO7647896.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SQHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKNLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18247..19569)
                     /gene="atoE"
                     /locus_tag="ACHKXP_002928"
     CDS             complement(18247..19569)
                     /gene="atoE"
                     /locus_tag="ACHKXP_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO7647897.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19566..20216)
                     /gene="atoA"
                     /locus_tag="ACHKXP_002929"
     CDS             complement(19566..20216)
                     /gene="atoA"
                     /locus_tag="ACHKXP_002929"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO7647898.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20216..20878)
                     /gene="atoD"
                     /locus_tag="ACHKXP_002930"
     CDS             complement(20216..20878)
                     /gene="atoD"
                     /locus_tag="ACHKXP_002930"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO7647899.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21074..22459)
                     /gene="atoC"
                     /locus_tag="ACHKXP_002931"
     CDS             complement(21074..22459)
                     /gene="atoC"
                     /locus_tag="ACHKXP_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO7647900.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRVKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22456..24282)
                     /gene="atoS"
                     /locus_tag="ACHKXP_002932"
     CDS             complement(22456..24282)
                     /gene="atoS"
                     /locus_tag="ACHKXP_002932"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO7647901.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24449..27298
                     /gene="rcsC"
                     /locus_tag="ACHKXP_002933"
     CDS             24449..27298
                     /gene="rcsC"
                     /locus_tag="ACHKXP_002933"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO7647902.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKLRC
                     LESGMDSCLSKPVTLDVIKQTLTLYAERVRKSRDS"
     gene            complement(27498..28148)
                     /gene="rcsB"
                     /locus_tag="ACHKXP_002934"
     CDS             complement(27498..28148)
                     /gene="rcsB"
                     /locus_tag="ACHKXP_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO7647903.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28165..30837)
                     /gene="rcsD"
                     /locus_tag="ACHKXP_002935"
     CDS             complement(28165..30837)
                     /gene="rcsD"
                     /locus_tag="ACHKXP_002935"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO7647904.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDNWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMIFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         7611 a         7684 c         8375 g         7509 t
ORIGIN      
        1 ggttattaac ttgaattatg acgcactgat attattcatc aaataataac aaaatagcca
       61 ttgcaccggg ttgaacaatt tacaaaagaa agatattcca tatcgtataa tgcgattaaa
      121 tacgccgtct tatagaaaat gatggttaga ttaatatgcg gattatcttt ctacgcaagg
      181 agtatttatc tttactcccg tcaatgattg catctctttt ctctgctaac ggtgtcgcgg
      241 cggtcactga ttcatgccag ggatatgatg tcaaagcgag ttgtcaggcc agcaggcaaa
      301 gcctttcagg cattacgcag gactggagta tcgctgatgg gcaatggctg gttttttcgg
      361 atatgaccaa taacgccagc ggtggggccg tatttttgca acaaggagcg gaattttcac
      421 tattaccaga aaatgaaact ggaatgactc tgtttgccaa taacaccgtt acaggagaat
      481 ataataacgg cggggccata tttgctaaag aaaactcaac gctgaatctt actgatgtta
      541 ttttttccgg taacgtcgca ggcggctatg gtggcgcaat ctattcttct ggtactaacg
      601 atactggtgc cgtcgattta cgtgtcacta acgccatgtt tcgcaataac atcgctaatg
      661 atggcaaagg tggcgcaatt tataccatta ataatgacgt ttatttaagt gatgttattt
      721 ttgataacaa ccaggcatat acatcaacaa gttacagtga tggcgatggc ggggcaatcg
      781 atgttaccga taataatagc gacagcaagc atccttcagg ttatacgata gtaaataaca
      841 ctgcctttac aaataacact gccgaaggtt atggcggggc gatatatacc aatagcgtga
      901 cggctcccta tcttattgat atttctgttg atgacagcta cagccagaac ggaggcgtgt
      961 tagtcgatga gaacaatagc gcagcaggct atggagatgg tccttcctct gcggcgggtg
     1021 gctttatgta tctcggctta agtgaagtta cctttgatat tgccgacgga aaaacgctgg
     1081 ttattggcaa tacagagaat gacggagctg ttgactctat tgctggtacc gggttaatca
     1141 ccaaaacagg ttccggcgat ctggtactta atgcagataa caatgacttt actggtgaga
     1201 tgcagattga aaacggtgaa gttaccctgg gccgcagcaa ctccctgatg aatgtcggcg
     1261 atacgcattg ccaggacgat ccgcaagact gctacggtct gacgataggg agtattgatc
     1321 agtatcagaa tcaggctgag ctaaacgttg gctcgaccca acaaactttt gtgcacgcat
     1381 tgacgggctt tcagaatggc actttaaata tcgatgctgg tggcaacgtt actgttaatc
     1441 agggcagttt tgctggcatc atcgaaggtg ctggtcagct caccattgcg caaaacggca
     1501 gctacgtgct ggcaggggcg cagtcgatgg cgctaaccgg cgatatagtc gttgatgatg
     1561 gtgcggtgct ttcgctggaa ggcgacgcgg cagatcttac cgctctccag gacgatccgc
     1621 agtcgatcgt gttaaacggc ggtgtgctcg atctctctga tttctccacc tggcagagcg
     1681 gcacatcata caacgatggc cttgaagtca gtggcagcag cggaacggtt atcggcagtc
     1741 aggatgtggt agatcttgca ggtggcgaca atttgcatat cggcggcgac gggaaagatg
     1801 gcgtctacgt ggtggtcgat gcgagcgacg ggcaggtaag tctggcaaac aataatagtt
     1861 atttgggcac aacacaaatc gcctccggta cgctgatggt gagcgacaac tcgcagcttg
     1921 gagataccca ctataaccgc caggttatct ttaccgataa gcaacaagaa agcgtgatgg
     1981 agattacctc cgacgttgac acgcgttcag atgcggcagg ccacggacgt gatattgaaa
     2041 tgcgcgccga cggtgaagtg gcagttgatg cgggggtaga cacgcagtgg ggcgcactga
     2101 tggctgacag cagcgggcag catcaggatg agggtagcac attgactaaa acgggggctg
     2161 gtacactgga gctgaccgcc agcggtacaa cgcagtcggc ggtacgtgtc gaagaaggca
     2221 ccctgaaagg tgatgttgct gatatccttc cttatgcttc gtcactgtgg gttggtgatg
     2281 gggcaacgtt cgttactggc gcggatcagg atattcagtc aattgatgct atttccagcg
     2341 gcactatcga catcagcgat ggtacggttt tgcgcctgac cgggcaggat acttccgtcg
     2401 cccttaatgc ctcactattt aacggcgatg ggacgctggt gaatgccacc gatggtgtga
     2461 cgttgacagg tgagcttaat accaaccttg aaactgacag cctgacttat ctttccaacg
     2521 tgacggttaa tggcaatctg accaatacgt ccggtgcggt tagcctgcaa aatggcgtcg
     2581 ctggcgatac gctgacggta aacggtgatt ataccggcgg cggtacgcta ctgctcgata
     2641 gcgaattaaa cggcgatgac tcggtaagcg atcaattggt gatgaacggt aatactgctg
     2701 gcaacacaac tgtggtggtt aactccatta cagggattgg tgagccgaca tcgacaggca
     2761 ttaaagtggt tgatttcgca gctgatccca cgcagtttca aaacaatgcg cagttcagtc
     2821 tggcaggcag cggctacgtc aatatgggag cgtatgacta cacgctggtg gaagataaca
     2881 acgactggta tctgcgatcg caagaagtaa cgccgccatc gccacctgag ccagacccga
     2941 ctcccgatcc tgatcccacg ccggatcctg acccaacccc cgacccggcc cctgtgcctg
     3001 tttaccagcc ggtgctgaat gccaaagttg gcggttatct caataacctg cgggcggcaa
     3061 atcaggcgtt tatgatggag cgacgcgatc acgcaggtgg cgatggtcag acgctgaatt
     3121 tacgtgttat cggcggagat tatcattaca cagcagcggg gcaactggct caacatgaag
     3181 acacttctac ggtgcaactt agcggcgatc tgtttagcgg gcgctggggc acggatggcg
     3241 agtggatgct tgggattgtt gatggctaca gcgataacca gggcgacagc cgctcgaata
     3301 tgaccggaac tcgcgccgat aaccagaacc acggttatgc cgttgggctg acatcaagct
     3361 ggtttcagca cggtaatcag aagcaagggg cctggctgga tagctggctg caatacgcgt
     3421 ggtttagcaa tgatgtttcc gaacaagaag atggcacaga tcattaccac tcgtcgggga
     3481 ttatcgcctc gctggaggcg gggtatcagt ggttaccggg gcgtggtgtg gtgattgaac
     3541 cgcaggcgca ggtgatttat cagggcgtgc agcaggatga ttttaccgcc gctaaccgtg
     3601 cgcgcgtgtc acaatcgcag ggtgatgata ttcagacgcg gctgggttta cacagcgaat
     3661 ggcgtaccgc tgttcatgtc ataccaacat tagatctgaa ttattatcac gatccccatt
     3721 cgacggaaat tgaagaggat ggcagcacta tcagtgacga tgcggtgaag caacggggtg
     3781 aaataaaagt gggagtcacg ggcaatatca gtcagcgagt gtccctgcgc ggcagcgtgg
     3841 cgtggcagaa agggagtgat gattttgccc agacggcagg gtttttgtcg atgacggtga
     3901 aatggtaatc actgtctagt cggcacggtt gccggatgcg gcgtaaacgc cttatccggc
     3961 ctacggggag catttgtagg cctgataaga cgcggcaagc gtcgcatcag gcattggcgc
     4021 ggccaaacat caacctcact tattctgcgt gtgcagcata tagttcacat ccacgccggg
     4081 gccgagttta aaagtattag tgatcgggtt gtaatgcagc ccagtgatat gccgctcttt
     4141 taaactggtc tgatccaccc agcccagcaa ttctgccggt ttaataaact tcttcacatc
     4201 atgcgtgcct ttgggcacca tgcgcaaaat atattccgca ccaaccaccg ccatcagcca
     4261 tgacttgccg ttgcggttaa gtgtcgagaa aaagacatcg ccgcctggtt tcaccagttg
     4321 cgcacaggct ctgaccactg actgcggatc ggggacgtgc tccagcatct ccatgcaggt
     4381 caccacatca tactgcccgg catgttttgc cgcgtgctct tccacggttt cctgcacata
     4441 atccacctga atgccgcttt ccagcgcgtg cagttttgcc acctgcaatg gctcaaagcc
     4501 catatccaga ccggtcaccg tcgcgccttc gcgcgccata ctctcggcca gaatgccgcc
     4561 gccacaaccg acatcgagca cctttttgcc aaataaaccg ccagcacgct cggcaatata
     4621 gcccagacgc agcgggttaa tgcggtgcaa cggtttgaac tcaccttcaa gatcccacca
     4681 gcgggaggcg acggcttcaa atttagcgat ctcttcgtgg tctacgttat ggttttccgg
     4741 cgatttttcg gcattcattg gcacttctac tccgtaattg gcaagacaaa cgagtatatc
     4801 aggcattgga tgtgaataaa gcgtataggt ttacctcaaa ctgcgcggct gtgttataat
     4861 ttgcgacctt tgaatccggg atacagtaga gggatagcgg ttagatgagc gaccttgcga
     4921 gagaaattac accggtcaac attgaggaag agctgaagag ctcctatctg gattatgcga
     4981 tgtcggtcat tgttggccgt gcgctgccag atgtccgaga tggcctgaag ccggtacacc
     5041 gtcgcgtact ttacgccatg aacgtactag gcaatgactg gaacaaagcc tataaaaaat
     5101 ctgcccgtgt cgttggtgac gtaatcggta aataccatcc ccatggtgac ttggcggttt
     5161 atgacacgat cgtccgtatg gcgcagccat tctcgctgcg ttacatgctg gtagacggtc
     5221 agggtaactt cggttccatc gacggcgact ctgcggcggc aatgcgttat acggaaatcc
     5281 gtctggcgaa aattgcccat gaactgatgg ccgatctcga aaaagagacg gtcgatttcg
     5341 ttgataacta tgacggcacg gaaaaaattc ccgacgtcat gccaaccaaa attcctaacc
     5401 tgctggtgaa cggttcttcc ggtatcgccg taggtatggc aaccaacatc ccgccgcaca
     5461 acctgacgga agtcatcaac ggttgtctgg cgtatatcga tgatgaagac atcagcattg
     5521 aagggctgat ggaacacatc ccggggccgg acttcccgac ggcggcaatc attaacggtc
     5581 gtcgcggtat tgaagaagct taccgtaccg gtcgcggcaa ggtgtatatc cgcgctcgcg
     5641 cagaagtgga agttgacgcc aaaaccggtc gtgaaaccat tatcgtccac gaaattccgt
     5701 atcaggtaaa caaagcgcgc ctgatcgaga agattgcgga actggtaaaa gaaaaacgcg
     5761 tggaaggcat cagcgcgctg cgtgacgagt ctgacaaaga cggtatgcgc atcgtgattg
     5821 aagtgaaacg cgatgcggtc ggtgaagttg tgctcaacaa cctctactcc cagacccagt
     5881 tgcaggtttc tttcggtatc aacatggtgg cattgcacca tggtcagccg aagatcatga
     5941 acctgaaaga catcatcgcg gcgtttgttc gtcaccgccg tgaagtggtg acccgtcgta
     6001 ctattttcga actgcgtaaa gctcgcgatc gtgctcatat ccttgaagca ttagccgtgg
     6061 cgctggcgaa catcgacccg atcatcgaac tgatccgtca tgcgccgacg cctgcagaag
     6121 cgaaaactgc gctggttgct aatccgtggc agctgggcaa cgttgccgcg atgctcgaac
     6181 gtgctggcga cgatgctgcg cgtccggaat ggctggagcc agagttcggc gtgcgtgatg
     6241 gtctgtacta cctgaccgaa cagcaagctc aggcgattct ggatctgcgt ttgcagaaac
     6301 tgaccggcct tgagcacgaa aaactgctcg acgaatacaa agagctgctg gatcagatcg
     6361 cggaactgtt gcgtattctt ggtagcgccg atcgtttgat ggaagtgatc cgtgaagaac
     6421 tggagctggt tcgtgaacag ttcggtgaca aacgtcgtac tgaaatcacc gccaacagcg
     6481 cagacatcaa cctggaagat ctgatcactc aggaagatgt ggtagtgacg ctctctcacc
     6541 agggctacgt taagtatcag ccgctttctg aatacgaagc gcagcgtcgt ggcgggaaag
     6601 gtaaatctgc cgcacgtatt aaagaagaag actttatcga ccgactgctg gtggcgaaca
     6661 ctcacgacca tattctgtgc ttctccagcc gtggtcgcgt ctattcgatg aaagtttatc
     6721 agttgccgga agccactcgt ggcgcgcgcg gtcgtccgat cgtcaacctg ctgccgctgg
     6781 agcaggacga acgtatcact gcgatcctgc cagtgaccga gtttgaagaa ggcgtgaaag
     6841 tcttcatggc gaccgctcac ggtaccgtga agaaaactgt cctcaccgag ttcaaccgtc
     6901 tgcgtaccgc cggtaaagtg gcgatcaaac tggttgacgg cgatgagctg atcggcgttg
     6961 acctgaccag cggcgaagac gaagtaatgc tgttctccgc tgaaggtaaa gtggtgcgct
     7021 ttaaagagtc ttctgtccgt gcgatgggct gcaacaccac cggtgttcgc ggtattcgct
     7081 taggtgaagg cgataaagtc gtctctctga tcgtgcctcg tggcgatggc gcaatcctca
     7141 ccgcaacgca aaacggttac ggtaaacgta ccgcagtggc ggaataccca accaagtcgc
     7201 gtgcgacgaa aggggttatc tccatcaagg ttaccgaacg taacggttta gttgttggcg
     7261 cggtacaggt agatgactgc gaccagatca tgatgatcac cgatgccggt acgctggtac
     7321 gtactcgcgt ttcggaaatc agcatcgtgg gccgtaacac ccagggcgtg atcctcatcc
     7381 gtactgcgga agatgaaaac gtagtgggtc tgcaacgtgt tgctgaaccg gttgacgagg
     7441 aagatctgga taccatcgac ggcagtgccg cggaagggga cgatgaaatc gctccggaag
     7501 tggacgttga cgacgagcca gaagaagaat aattttactt cttcatgcca aaagggagct
     7561 atctcccttg tttgaattga aaagtccagg ctgcaaagtc tgggcttttg tcgtattagg
     7621 gcacggtaaa gtttggctgt gcccgtaaaa aatggctggc tatacacaag gaatgtggca
     7681 atgagtggtg aaaaaaaggc ggaaggctgg cggttctatg gtcttgtagg ttttggcgca
     7741 atagcactgc tttccgctgg cgtctgggcg ttgcaatatg ctggcagtgg gccagaaaaa
     7801 acgttgtcgc cgctggtggt gcacaacaat ctgcaaatcg atctcaatga gccggacctc
     7861 tttctcgaca gcgactctct gagccagctt cccaaagatc tcctcaccat tccgtttctc
     7921 cacgatgttc tgagcgaaga tttcgttttc tattatcaga atcatgccga tcgtctgggc
     7981 attgaaggca gcattcgtcg tattgtctat gaacacgatc tcacgctgaa agataagctc
     8041 ttttcgtcac tcttagatca gcccgcgcag gcagcgctgt ggcacgataa acaaggccat
     8101 ctttcacatt atatggtgct gatccagcgc agtggtttaa gcaaactgct ggagccattg
     8161 ttgtttgccg ctaccagcga cagccagtta agcaaaacgg aaatcagtag catcaagata
     8221 aatagtgaaa ctgttcctgt ttatcagttg cgctataacg gcaataacgc tctgatgttc
     8281 gcgacatatc aggacaagat gctagtgttt tccagcacgg atatgctgtt taaagatgat
     8341 cagcaggata ccgaagccac ggcgatcgca ggtgatttgt tgagcggcaa aaaacgctgg
     8401 caagcaagct ttggcctgga agagcgtgct gctgaaaaaa cgccagtacg ccagcgcatc
     8461 gtggtcagcg ccaggttgct gggttttggc taccagcggt taatgccttc ttttgctggc
     8521 gtacgcttcg aaatgggtaa cgacggctgg cacagttttg tggcgttaaa tgatgaatcc
     8581 gccagcgtag ataccagttt cgattttacg ccggtctgga acagtatgcc tgccggggcc
     8641 agcttctgtg tggcggtgcc gtattcacac ggtattgccg aagagatgct ttcgcacatc
     8701 agccaggaaa acgacaagct gaatggggcg ttagacggtg ccgcggggct gtgctggtat
     8761 gaagactcaa aattgcaaac cccgctgttt gtcggtcagt ttgatggcac tgccgaacag
     8821 gcgcaattgc cagggaaact gtttacgcaa aatattggtg cgcacgaaag caaagcgcca
     8881 gaaggtgttt tgccgataag ccagactcag cagggcgaag cgcaaatctg gcgtcgcgaa
     8941 gtgagttccc gatacggtca gtatccgaaa gcgcaggcgg cgcaacccga tcaattaatg
     9001 tcggattatt ttttccgcgt gtcgctggcg atgcaaaaca aaacgctgct tttctccctc
     9061 gatgacacgc tggttaataa cgccctgcaa acactgaata aaaaccgccc ggcaatggtg
     9121 gatgtaatac ccactgatgg catcgttccg ctctatatca atccacaagg cgtggcgaaa
     9181 ctgctgcgta acgaaacgtt gaccagtctg ccgaagaatc tcgaaccggt tttttataac
     9241 gccgcacaaa ctttattaat gccgaagctg gacgctttat ctcaacaacc gcgttatgtc
     9301 atgaagctgg cccagatgga acccggtgcc gcctggcagt ggctacccat aacctggcag
     9361 ccgctatgag gcacgggctg ctggcgctga tttgctggct gtgttgtgtt gttgcccata
     9421 gcgaaatgct gaatgtcgaa caagccgggc tgtttcgcgc ctggtttgta cgcattgcac
     9481 aagaacagct ccgccagggg ccaagtccac gctggtatca gcaggattgt gcgggcctgg
     9541 tgcgatttgc ggcgaacgag acgctgaaag ttcacgacag taaatggcta aaaagtaacg
     9601 gtttatctaa ccagtatttg ccgccagaga tgacgctaac acctgaacag cgtcaactgg
     9661 cacaaaactg gaatcagggg aacgggaaaa ccggccccta cgtgaccgcg attaatttga
     9721 ttcagtacaa cagccagttt attggccagg acataaacca ggcgctgcct ggcgatatga
     9781 tttttttcga tcagggcgat gcccagcact taatggtctg gatggggcgt tacgtcatct
     9841 accacaccgg aagcgccacg aaaactgaca acggaatgcg cgcagtcagt ctgcaacaac
     9901 ttatgacatg gaaggacacc cgatggatac ccaacgattc caatcccaat ttcattggca
     9961 tttatcgttt aaattttctg gcgcgatagc agcgtgcttg tccctgtctc ttgtgggaac
    10021 agggcttgct aaagctgatg attcgcttcc ttccagtaac tatgcgccgc ccgccggggg
    10081 aacattcttt ttgcttgctg acagcagttt tagcagcagt gaagaggcga aagtgcgact
    10141 ggaagcgccg gggcgtgatt atcggcgcta tcagatggaa gagtacggcg gcgtggacgt
    10201 tcgcctgtat cgtattcctg acccgatggc atttttgcgc cagcagaaaa acctgcatcg
    10261 cattgtggtg caaccgcaat atctgggcga cgggctgaac aatacgctga cctggctgtg
    10321 ggataactgg tacggcaaat ctcgccgcgt gatgcagcgt actttctctt ctcagtcacg
    10381 gcagaatgtg actcaggcgt tacccgaatt acagctcggc aatgccatta ttaaaccttc
    10441 ccgttatgta cagaacaacc agttttcccc gctgaaaaaa tatccactgg tggaacagtt
    10501 ccgttaccca ttatggcaga ctaaaccggt cgagccgcag caaggggtaa aactggaagg
    10561 cgcatccagc aatttcatct cgccgcagcc gggtaacatt tatattcctc tcggccaaca
    10621 agagccggga ctgtacctcg tcgaggcgat ggttggtggg tatcgggcga cgacggtggt
    10681 gtttgtttcc gataccgtgg cgcttagcaa agtgtcaggt aacgagcttc tggtatggac
    10741 cgcgggtaaa aaacagggtg aagcgaagcc cggctcagag atcttgtgga ctgacggtct
    10801 tggcgtgatg acccgcggtg tgaccgatga cagcggtacc ttgcagttac aacatatatc
    10861 gccagaacgt tcatacattc tgggtaagga tgctgaaggc ggcgtttttg tctccgagaa
    10921 cttcttctac gaaagcgaaa tctacaacac ccgcttgtat atttttaccg atcgcccgct
    10981 atatcgcgca ggcgatcgtg tcgatgttaa agtgatgggc cgcgagttcc acgatccgtt
    11041 gcattcatcc cccatcgtca gcgccccggc gaagctttcg gtgctggacg ctaacggcag
    11101 tctgttgcaa accgtcgatg tcacgctgga tgcgcgcaat ggcgggcagg gaagtttccg
    11161 cctgccagaa aatgccgtag ccggaggtta tgagttacgt cttgcttacc gcaatcaggt
    11221 ctatagcagc agttttcgcg tggcaaacta catcaagcca catttcgaga ttggtttagc
    11281 tctcgacaaa aaagagttca aaactggcga agcggtcagc ggcaaactgc aactgctcta
    11341 cccggatggc gagccggtaa aaaatgcccg cgtgcagtta agtttgcgcg ctcagcaatt
    11401 atcaatggtc ggtaacgatt tgcgttatgc cggacgtttc cccgtgtcgc tggaaggcag
    11461 cgaaacggtg tccgacgcca gcggtcatgt gacgttaaat ctccccgccg ccgataaacc
    11521 gagccgctat ttgttaaccg tctccgccag tgacggcgcg gcgtatcgcg tcaccaccac
    11581 caaagagatc ctcattgaac gcggcctggc gcattactct ttaagtaccg ccgcacaata
    11641 cagtaatagc ggcgagtcgg ttgtgttccg ttatgccgcg ctggaatctt caaaacaggt
    11701 tcctgttacg tatgaatggt tgcgtctcga agaccgcacg agccatagcg gagagctacc
    11761 gtcaggcggc aaatccttta ccgtcaattt cgctaaacct ggcaactaca atctgacatt
    11821 acgcgataaa gacggcttaa ttctcgctgg gttaagtcat gccgtcagcg gtaagggcag
    11881 cacggcgcat actggtacgg tagatatcgt ggcggataaa acgctgtacc agccaggcga
    11941 aaccgcgaag atgctgatta cctttccgga gccaattgat gaagcattat tgacgctgga
    12001 acgcgatcgc gtggaacagc agtcgctgct ttcgcatccg gcaaactggc taacgctaca
    12061 acgtttaaac gatacccagt atgaagcccg ggttccagtg agcaattcct ttgcgcctaa
    12121 catcactttt tcggtgctgt atacccgtaa cggtcagtac agttttcaga acgccgggat
    12181 caaagttgcc gttccccagc tggatatccg ggtgaaaacg gacaaaaccc attaccagcc
    12241 tggtgaactg gtcaatgtcg aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca
    12301 gctaacggta ggcgtggtcg atgaaatgat ctacgcgctg caaccagaaa tcgcgccgaa
    12361 tatcggcaaa tttttctatc cgctggggcg taacaatgtg cgtaccagct ccagtctgtc
    12421 gtttatcagc tacgaccagg cgctctccag cgagccggtt gcgcctggcg caactaaccg
    12481 cagcgagcgg cgagtaaaaa tgcttgaacg tccacggcgt gaagaggtgg ataccgcggc
    12541 atggatgccg tcactcacaa ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc
    12601 tgattcgtta acccgctggc gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg
    12661 gcaggggcgt gcttatctgc gttcggaaaa aaatctctac atgaagtgga gtatgccaac
    12721 ggtgtatcgc gtgggcgaca aaccggcggc aggactgttt atcttcagtc agcaggataa
    12781 cgaaccggtg gcgctggtga ctaaatttgc aggcgctgag atgcgccaga cgctgacgct
    12841 gcacaaaggg gcgaattata tttcgctgac gcagaatatt cagcaatctg gcttgttaag
    12901 tgcagaactg caacaaaatg ggcaagtgca ggacagcatt agcacaaaac tgtcttttgt
    12961 ggataacagc tggcccgttg aacagcagaa aaatgtcatg ctcggtggtg gcgataacgc
    13021 gctgatgttg cccgagcagg cgagcaatat ccggctacaa agtagtgaaa cgccgcagga
    13081 gattttccgc aacaatcttg atgcgttagt cgatgaaccg tggggtggcg taatcaacac
    13141 cggtagccgt ctgatcccgc tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc
    13201 cgccgctaac gacattcgtc agatgattca ggataaccgt ctgcggctga tgcaactggc
    13261 ggggcccgga gcgcgcttta cctggtgggg tgaagatggc aatggtgacg ccttccttac
    13321 ggcatgggca tggtacgccg actggcaggc cagccaggcg atcggcgtaa cgcaacaacc
    13381 ggaatactgg cagcatatgc tcgacagcta cgcggagcag gcagataaca tgccgttatt
    13441 gcatcgggcg ctggtgctgg catgggcgca ggagatgaat ttgccgtgca aaacgttgtt
    13501 gaaagggttg gatgaagcta tcgcccggcg cggaactaaa actgaagatt tctctgagga
    13561 agacacccgc gatatcaatg atagcctgat cctcgataca ccggagtctc cactggcaga
    13621 tgcggtggca aacgtcttaa ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt
    13681 gatgccacag gttcagcaat atgcgtggga taaagcggca aacagcaatc agccgctggc
    13741 gcacacggtt gtgctgctta atagcggtgg cgacgctacc cagacggccg ctattttaag
    13801 tggtttgacc gctgagcaat ccactattga gcgcgcgctg gccatgaact ggctggcgaa
    13861 atatatggcg acaatgcctc cagttgtttt gcctgcgcct gcgggcgcat gggctaaaca
    13921 taagttaact ggagggggcg aagactggcg ttgggttggt cagggcgtgc cggacattct
    13981 ctcttttggt gacgaattat cgccgcaaaa tgtgcaggtc cgctggcgtg agccggcaaa
    14041 aatggctcaa caaagtaaca ttccggtgac cgttgaacgc cagttgtatc ggcttatccc
    14101 tggtgaagaa gagatgagct ttattctgca accggtgacc agcaatgaga ttgacagcga
    14161 tgcgctgtat ctcgatgaaa tcacgcttac cagcgagcag gatgcagttc tgcgctacgg
    14221 tcaggtggaa gtaccgctgc caccgggagc cgacgttgag cgcacaacat ggggcatttc
    14281 ggtcaataaa cccaacgccg cgaaacagca ggggcaattg ctggaaaaag cgcgaaatga
    14341 aatgggcgaa ctggcctata tggtgccggt gaaagaactg acgggaacgg tcactttccg
    14401 ccatttgctg cgcttctcgc aaaaagggca attcgttctg cctcctgctc gttatgtgcg
    14461 ttcctatgca cctgtgcagc aaagtgttgc ggcagggagt gaatggaccg ggatgcaggt
    14521 gaaataagtg aactggcgca gaatcgtctg gctattggcg ttggttactc tgccaacact
    14581 tgcggaggag acgccgttac aactggtgct gcgcggtgcg cagcacgatc aactgtatca
    14641 gctgtcgtca tcaggcgtga ccaaagtctc agcattacct gactcgctca ccacgccgtt
    14701 agggagtttg tggaaactgt atgtttacgc ctggctggag gatacccatc aaccggagca
    14761 gccctaccag tgtcgcggaa actccccgga agaggtttat tgttgtcagg ctggggagag
    14821 cataacgcgc gataccgcgc tggtacgttc ctgtgggctc tattttgcgc cgcagcggtt
    14881 gcatattggc gcagatgtgt gggggcaata ctggcaacag cgtcaggcac ctgcgtggtt
    14941 agcgtctctg actacgctga agccagaaac ctcggtaacg gttaaatcgc tgcttgattc
    15001 attagcgacg ttacccgcgc aaaacaaagc tcaggaagtc ttactggatg tggtgctgga
    15061 cgaggcgaaa atcggtgtcg catccatgct cggtagtcgg gtacgggtga aaacctggag
    15121 ctggtttgcc gatgataaac aggaaatccg tcagggtggt tttgctggct ggttaaccga
    15181 tggtacaccg ctgtgggtta ctggcagtgg caccagcaaa acggtactaa cccgttatgc
    15241 gacggtgctt aatcgggttt tgccagtgcc gacgcaggtt gccagtgggc aatgtgtaga
    15301 ggtggagctg tttgcccgct atccgctgaa gaaaatcaca gcggaaaaga gtacgacggc
    15361 agtaaatccc ggcgtactta atgggcgcta tcgtgtgacg tttactaatg gtaaccacat
    15421 tacgtttgtc agccacggtg agacgacctt actgagcgaa aagggcaaac tgaaattgca
    15481 gtcgcatctc gatcgtgaag agtacgttgc cagagttctg gatcgcgagg cgaaaagtac
    15541 gccccctgag gcagcgaaag ccatgaccgt ggcgattcgc acttttttac agcaaaatgc
    15601 caatcgggaa ggcgattgcc tgaccatccc cgacagcagc gccacgcagc gtgtttctgc
    15661 ttcgccagcg accacggggg cgcgaacgat gaccgcctgg acgcaggatc tcatctatgc
    15721 aggcgatccg gttcattatc atggcagtcg cgctaccgaa gggacacttt cctggcggca
    15781 agccacggcc caggcggggc agggagagcg ttacgatcag atactcgcgt ttgcttatcc
    15841 cgacaacagt cttagccgct ggggagcgcc acgctcaacc tgccagttat tacccaaagc
    15901 gaaagcctgg ctggcgaaaa aaatgccgca gtggcggcgt atattacaag ctgagacggg
    15961 gtacaacgaa ccagacgtgt ttgcggtttg ccgtttagtc tcaggtttcc cctataccga
    16021 tcggcagcag aaacggctgt ttattcgtaa cttctttacg cttcaggatc ggctggattt
    16081 aacccatgag tatctgcacc tggcctttga tggttatccc accgggcttg atgagaacta
    16141 tatcgaaacg ctgacccgac aattattaat ggactaatgc tatgcgaaag atttttcttc
    16201 cgttgttact ggtggcgctt tcgcctgtcg ctcacagtga aggtgtgcag gaagtcgaga
    16261 ttgatgcacc gctctcaggc tggcatccgg cagagggcga agatgccagt ttttcacaat
    16321 cgatcaatta cccggcgtcg tccgtcaaca tggcagatga tcaaaatatc tcagcgcaga
    16381 tccgcggcaa aataaagaat tatgctgcgg cgggtaaagt tcagcagggc cggctggtgg
    16441 tcaacggtgc cagtatgccg cagagaattg aatccgatgg ttcatttgca cgcccttata
    16501 ttttcactga aggcagcaac agcgtgcagg tcatcagccc ggatgggcaa agccgacaaa
    16561 aaatgcagtt ttactcaacg ccgggtacag gaacgattcg tgcacgttta cggctggttc
    16621 tctcgtggga tacggacaat accgacctcg accttcacgt tgttacgccc gatggcgaac
    16681 acgcctggta cggtaacacc gtgctgaaaa acagtggtgc actggatatg gatgtcacga
    16741 cggggtacgg ccccgagatt ttcgccatgc ccgcgccaat tcacgggcgc tatcaggtgt
    16801 atatcaacta ctatggtggg cgcagcgaaa cggaattaac caccgcccaa ctgacgctga
    16861 tcaccgatga aggatcggtc aatgagaaac aggaaacatt tattgtaccg atgcgtaatg
    16921 ctggcgagct gacgctggtg aaaagttttg actggtgatt aatggggtta ccttcgccgt
    16981 cgatgttcaa gaaaacaccc gataactttc gctatcgggt gtttttattg attaattcaa
    17041 ccgttcaatc accatcgcaa ttccctggcc gccgccaatg cacagtgttg ccagccccag
    17101 cgttttatcg cgtgcctgca tggcatgtaa tagtgtgacc agaatacgag caccactggc
    17161 accgatagga tgcccgagcg cgatggcccc gccgttgaca ttcactttct cagaatcaaa
    17221 gcccaggttt ttcccaacgg caaggaactg tgcagcaaat gcttcattag cctcaatgag
    17281 atcaatatcc gccagttgca gccccgccag ttgtaacgct ttttgcgtgg caggtactgg
    17341 ccccataccc atcaatgcgg ggggcacgcc accgctggca taacttttaa tgcgagccag
    17401 gggggtaagg cctgctgcca gcgccgcaga ttcttccata atcaccagag cggcagcacc
    17461 gtcgttaata ccagacgcgt tcccggcggt aactgttcct gctttatcga aggccgggcg
    17521 caatgcgcct aacgcttcag tcgtagaatc cgctttcggg aattcgtctt gactgaagac
    17581 gaaggttttc ttccgagtga caacatttac cgggacgatt tcggctgtaa aagcaccgga
    17641 ctcaattgcg gctgccgctt tacgctgtga atgtagcgcc agttcatcct gcatttctcg
    17701 ggtaattccg tactctttgg ccacgttctc ggcggtaatc cccatatgat aaccatgggt
    17761 ggcgcacatc aggccatcgc gcaggattac gtcataaacc tgtccgtctc caagacgata
    17821 accagagcgt gcttttgcat cgagtaagta gggggctaaa ctcatatttt ccataccccc
    17881 cgccacaatg ctctgcgcct gacctgcctg aatggcctgg gcggcaagcg ccacactttt
    17941 aagacccgaa ccacatactt tattgaccgt gaatccgcac accgtttctg ccagcccgct
    18001 ttttaacagt gcctgacgcg ccggattttg ccccagcccg gcttgtaaca cgttacccat
    18061 aatcacttca tcaacgtgtt gtgaatcgat ttttgcacgt tcaatggcgg ctttaattac
    18121 tgtcgccccc aggtcgatgg cgctggtgga agcgagtgaa ccgttaaaac taccgatagc
    18181 agtacgtacc gcactgacga tgacacaatt tttcattttt tattccttct gtctgtaggg
    18241 ttgccgtcag aacagcgtta aaccaatgac gaaaatgacg ccggagaaga gcagggcagt
    18301 gatgcaatag cccatgatgt cgcggacacc gagtccggcg attgccagtg ctggcagcgc
    18361 ccagaatggt tgtgccatgt tcatccattg ctcgccgtag gcgatcgcca ttaccgattt
    18421 accgagatca gcgcccagcg cctgggctgc gggtatcacg aaaggtccct gaataaccca
    18481 gtgaccgccg ccagacggaa cggcgaagtt aatcagtgca gaactaaaaa aggtcattac
    18541 cgggaaggtg tctttgttcg caacattgat gaagaattcg gtaatgagtc cgcccagacc
    18601 ggaatgctcc atcatcagtt ggatcccagc gtagaagggg aattgcacca gaataccggc
    18661 agtactgcgt gctgccgcgc tgatagcacg catataagcc attggcgttt tatgtagcag
    18721 cagacccgca atcataaaca tcaggttgac ggtattgatg gtgatgttga agccatgttc
    18781 gctgaagtac atcgcaaggt aagcgatacc gagtgcgccg atgatcaacg tcagaatgcg
    18841 gctttcttcc agtcgctcgg atggtggggc atctttcggt agctgctttt gaaaatcagc
    18901 ctcttccatg agtagtttcg gatcgatact caccacgtca gacggttttg gcatcatcat
    18961 gcgggtgata aatggcatca ccacaatcaa cgccacagtg atgaaaatat taaaaccact
    19021 gaacagagta tcgcccaccg ggatcagccc ggcgatatgc tcaaccgggt tgcccggtgt
    19081 tgcagccaac agaggcattg atccagagaa gccgccaccc caggtgagaa aaccaatgta
    19141 ggcgcaggca atgagcaacg gataatcaga accggggact cgccgggcga cttcacgggc
    19201 aaacattgcg ccgacaacca aaccaaatcc ccagttgatg acacaagcga ctgaaccgaa
    19261 gaaagtgacc agcatgacgc cctgtacggg cgtctttgcg gcggaggcgg cagtacgcag
    19321 caaacttttc accggagcag agctggcaag ggcatgaccg gtaacgatga taagcgccat
    19381 ctgcatacca aacgccagca agttccagaa accgtcaccc cacattttca ccatgctgat
    19441 cggcgtttgt ggtgttaacc aaagcgcgat cacgaatgtt agcaatgtca gcaacatggc
    19501 aaagatcagt ggatcgggaa gccaccggct gacaaaacgc gtcataaaac gcgatatgcg
    19561 accaatcata aatcaccccg ttgcgtattc agatcggcgg cgacttcaaa ccgagcttct
    19621 gttttggcac gcacggtggc taaatcacac ccgtcggcaa tttcggtgag ccacattttg
    19681 ccgtcaataa aacgaaagac agccagttca gtaaccagca tatgcaccgc atgttgtgca
    19741 gtgagtggca tggtgcagcg gcgcaaaatt tttgctgaac catctttggc gcaatgttcc
    19801 atggcgatga tcactttgcg cgacccggtc accagatcca tcgcgccacc cataccgggc
    19861 accattttcc ctggcactac ccagttcgcg aggtttgctt cttcgtctac ttgcaaaccg
    19921 ccgagcacgc aggcatcaat atgaccgcca cggattagcg caaatgacat ggcgctatca
    19981 aacatggctg caccgggtaa aacaccgcac ggttgtccgc cagcgttcac cagatctgga
    20041 tgcgctgtcg tgaccgggcc taaaccgagg aagccgtttt ccgattgcag agtgatatga
    20101 ataccctccg gtaaataatt ggcgaccatt gtgggtaaac cgatccctaa gttaacgatg
    20161 tcaccatcac gaagctcttg cgccacacgg cgcgcaatac gttgtttcgc atccattatt
    20221 tgctctcctg tgaaacgatg atgtggtcga taacggcacc aggggtgaca atatggtcag
    20281 gttgcagctc gccggtttcg accagttcat ctggctctac cagcgtgata tcagctgcaa
    20341 gggctatcag ggggttaaag ttgcgggcgc taagttgata ggtcaggttg ccaagtgtgt
    20401 cgcaacgatg agcgcgaatt agcgccaggt cggcgcgcag tgggcgttcg agcagccagg
    20461 ttttaccgtc gagtgtcagt gtctgtttgc cttcctctac gacggtgccg acacccgttg
    20521 gggtgagaaa accaccaagt ccagctccac cacagcgaat ttgctcgatt agcgtacctt
    20581 gcggcaccag aacgacgtcc atctcaccag atatcatgcg ccgacctgtt tccgggttgg
    20641 tgccgatatg tgaagcaatc actttgcgga ctcgaccatt gacgatgagc ggaccgatgc
    20701 cggtatcaac aaacgcggta tcattggcta tcaatgtcag gtcgcgaaca ccagattcca
    20761 gtaatgcttc aaccaggcgg gatggagtgc caatccccat aaatccgccc accatgatgg
    20821 tcatgccgtc acgaaagaag ccggtggcgt cttgtaatgt catcaatttt gttttcattt
    20881 tttatccctc ttgcatacac cgttgtgggt acagtcaggc aatagcaagg ggagtgccag
    20941 aatgagatta ttggatttag ttatttaact aattgaatat gaagggataa attggatggg
    21001 ggtttataag gagaatgagc ggctatgcag aaaattgcgc actgtgcaaa tttctgcata
    21061 gcaagttttg gtgttataca tccgccggat cgataccgta ttcctggagt ttatacatca
    21121 atgcacggcg actgatgccc agcattaaag cagtgcgggt tcggtttcct tcttgttgtt
    21181 ccagcacttc cataatgatg cgtttttcga cgcgtttaat ttcctctttt aaattacgct
    21241 caccgacagg ggctgttttt acctcgccag cattacagac tggctgacga atctgtggcg
    21301 gaagatcctc agaaaaaatg atcgggcctg aattcatcac gacggcgcgt tcaataacgt
    21361 tggaaagctc tcgaatattt cccggccatg accaggcggt aagcagtgac attgccatcg
    21421 gatcgatgtc gataatatcg cgctgattct cactactgaa tttttgcaaa aagtgattag
    21481 ctaacaggga aatatcttcc cggcgatcgc gcagaggcgg cagtattaaa tgaataacgt
    21541 taaggcgata aaagagatct tcacggaagg tgccttcttt taccattgcc tgcaagtcgc
    21601 ggttggtggc agcaatgatg cggatatcaa cttttatggt ctgatggccg ccaatccgtt
    21661 caaattcccg ttcctgtaga atgcgtagta atttggcttg tagtaccagc ggcatttcgc
    21721 caatttcgtc gaggagcaga gtaccttcgt tggctcgttc aaataatccc tgacgcaagg
    21781 tttgtgcacc agtaaatgca cctttttcat gaccaaacag ttcactttcg agcaacgatt
    21841 ccggcagcgc cgcgcagttg actttaatga acggcccctt tacccgccgc gaattgtagt
    21901 gaatcgctct ggcaatcaac tctttcccgg tgccgctttc accgctaatc aagacgctgg
    21961 cctgagaaag ggcaattttg gcggtgtctt tgcagatgtc catcatcgcc gggctgttgg
    22021 tgagaatgtg cccccattgc cagctggtgc tcagtgcctg gtgcagatga cggatctctt
    22081 ttttcattga ctggagttgt aaagcgcgct gaacgattaa attcaactca tcgagatcaa
    22141 acggtttaat aacatagtcg aaggctccgc agcgtagcgc ttcgacggcg gtttccactt
    22201 ccgcataggc cgtcatcaga ataacgggtg tccgggtctc atggctgcgc atctccttta
    22261 gtgccttgat gccgtccatc tctggcatgc ggatatccat caacaccaca tcagggtgaa
    22321 tatcggcaaa caggtgtaat gctgtgcgtc cgttgttcgc acaatgtgtt tcgaatcctt
    22381 gtagtgcaaa agcggtgctc agcatacggc gaacattatc ttcatcatcc acaataagga
    22441 tgcgattaat agcagtcata cagtctgatt tccctgcggg ttgatcggta aaataagcgt
    22501 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
    22561 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
    22621 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
    22681 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
    22741 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
    22801 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
    22861 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
    22921 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
    22981 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
    23041 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
    23101 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
    23161 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
    23221 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
    23281 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
    23341 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
    23401 gataacttct gctgccgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
    23461 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
    23521 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
    23581 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
    23641 caggcgacgg gagaaaagga caatcaacag caggcttatc agcaaaccgg cggtgagcac
    23701 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
    23761 ggcccagata tagccgagga tttcaccatt acgctcaatg gggatcattg aattcaaaat
    23821 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
    23881 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
    23941 gtaggtgatt atcgcatcca gcgttttgtt gtaataacca gcgccgatgc cagggaaggc
    24001 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
    24061 acgcggtaag tcgatgtaga gatcgtagcg attgcctagc gcctgattaa gcaggttgac
    24121 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
    24181 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
    24241 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
    24301 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
    24361 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
    24421 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
    24481 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
    24541 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
    24601 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
    24661 agagctgaag tacatcgccg aaaatcgctt atcggcagag aacggtgtgc tttccccgcg
    24721 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
    24781 ttccgcgatg agtaacactt ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
    24841 ctggcgcgat aatttttctg cggcttacga tctcaaccgg gtatttttaa tcggcagtga
    24901 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
    24961 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
    25021 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
    25081 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
    25141 ggaaaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
    25201 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
    25261 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
    25321 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
    25381 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
    25441 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
    25501 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
    25561 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
    25621 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
    25681 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
    25741 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
    25801 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
    25861 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
    25921 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
    25981 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
    26041 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
    26101 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
    26161 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
    26221 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgttcg
    26281 cgcggatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga taccggcgaa
    26341 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgtaattt
    26401 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
    26461 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
    26521 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
    26581 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
    26641 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttga tcactgacga
    26701 ggtagtgagt aaaaaatggc agggcagagc ggtagtgacc ttctgtcgtc gccatattgg
    26761 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgctc cgcatgagct
    26821 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
    26881 tctgccgtca acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
    26941 tgatcatccg attaaccggc gtttgctggc agatcagttg ggatcgttgg gctatcaatg
    27001 taaaaccgcg aatgatggcg tcgatgcgct taatgtactt agcaagaatc atattgatat
    27061 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
    27121 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
    27181 gctgcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
    27241 gataaaacag acgctgacgt tatatgccga gagggtcagg aaatcgcggg attcgtagga
    27301 cggataaggc gtttacgccg catccggcat tcagtgcaaa tgccagatgc gacgctgacg
    27361 cgtcttatct ggcctacttt aatgtaattt gttgaaataa tgggaatcgt aggccggata
    27421 aggcgtttat gccgcatccg gcaatcggtg caaatgccag ataagacact aacgcgtctt
    27481 atctggccta caggtgatta gtctttatct gccggactta aggtcactga agagagataa
    27541 ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta
    27601 ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc
    27661 gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca
    27721 gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc
    27781 agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac gatcccttcg
    27841 atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt cagaacaatg
    27901 atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcgccgtac
    27961 ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt cggcaggttg
    28021 ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca
    28081 agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata
    28141 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
    28201 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
    28261 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
    28321 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
    28381 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
    28441 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
    28501 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
    28561 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
    28621 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
    28681 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
    28741 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggtc
    28801 agctgcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
    28861 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
    28921 cgccttgcca tagcgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
    28981 ttcatgaata cttacgcctt ctcccgtgtc cagaatgcgg aacgtcaggc ggtcttcgga
    29041 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
    29101 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
    29161 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
    29221 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
    29281 gctatcatcc gcaagcatgt tcgctaactg tatttcatca accagccgca ccagcacatc
    29341 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
    29401 ggactgtgcg ggttctttca gcgcatcgcc aatgtttttc ataaagatca tccgcccctg
    29461 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
    29521 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
    29581 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat
    29641 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
    29701 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
    29761 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgc tggggacgtt
    29821 ttctgtactg cggctggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
    29881 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
    29941 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
    30001 aatcttcgta ctgttaaagt ggatactgac gctatccgtc ccttctttct cattatcatt
    30061 gtttcccgtt gccgtcgcgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
    30121 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ctggctggtt
    30181 aaaggtagta cgcaatgtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
    30241 aaagctttcg cgttcatcga gggcgttggc ctgttgcaac atctctgcac gacgtgaatc
    30301 aacaatgtcg ctgacggtcg attctttaaa tccggaggtg agatctttaa ggggtagggt
    30361 tgagatcagc accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt
    30421 ttctgcgccc cacaatgtat ccagataagt ggacatccgc tgagtcatct caagcgttga
    30481 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta
    30541 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
    30601 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag ttatcgacgc gcttttggat
    30661 agcatgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
    30721 ttgtaccatt acacccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
    30781 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
    30841 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
    30901 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
    30961 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
    31021 cgaaaataat tgcaaataaa caaagattgc tggaaattat gctgatgtta attatttgtg
    31081 aaatagttaa caagcgttat agtttttatg tggtagcaca gaataatgaa aagtgtgtaa
    31141 agaagggtaa aaaaaaccga atgcgaggca tccggttga
//
LOCUS       DAZFZC010000058        30896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFZC010000058 DAZFZC010000000
VERSION     DAZFZC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.058"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(296..2869)
                     /gene="clpB"
                     /locus_tag="ACHKXP_002936"
     CDS             complement(296..2869)
                     /gene="clpB"
                     /locus_tag="ACHKXP_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO7647905.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="ACHKXP_002937"
     CDS             complement(2999..3730)
                     /gene="yfiH"
                     /locus_tag="ACHKXP_002937"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO7647906.1"
                     /translation="MSKLIVPQWPQPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGGEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="ACHKXP_002938"
     CDS             complement(3727..4707)
                     /gene="rluD"
                     /locus_tag="ACHKXP_002938"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO7647907.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4842..5579
                     /gene="bamD"
                     /locus_tag="ACHKXP_002939"
     CDS             4842..5579
                     /gene="bamD"
                     /locus_tag="ACHKXP_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO7647908.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5850..6191
                     /gene="raiA"
                     /locus_tag="ACHKXP_002940"
     CDS             5850..6191
                     /gene="raiA"
                     /locus_tag="ACHKXP_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO7647909.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6294..6419
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6295..6342
                     /gene="pheL"
                     /locus_tag="ACHKXP_002941"
     CDS             6295..6342
                     /gene="pheL"
                     /locus_tag="ACHKXP_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFO7647910.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            6441..7601
                     /gene="pheA"
                     /locus_tag="ACHKXP_002942"
     CDS             6441..7601
                     /gene="pheA"
                     /locus_tag="ACHKXP_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO7647911.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSDTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="ACHKXP_002943"
     CDS             complement(7644..8765)
                     /gene="tyrA"
                     /locus_tag="ACHKXP_002943"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO7647912.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLVAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="ACHKXP_002944"
     CDS             complement(8776..9846)
                     /gene="aroF"
                     /locus_tag="ACHKXP_002944"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO7647913.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKSI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10056..10421
                     /gene="yfiL"
                     /locus_tag="ACHKXP_002945"
     CDS             10056..10421
                     /gene="yfiL"
                     /locus_tag="ACHKXP_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO7647914.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10571..11089
                     /gene="yfiR"
                     /locus_tag="ACHKXP_002946"
     CDS             10571..11089
                     /gene="yfiR"
                     /locus_tag="ACHKXP_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO7647915.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11079..12305
                     /gene="dgcN"
                     /locus_tag="ACHKXP_002947"
     CDS             11079..12305
                     /gene="dgcN"
                     /locus_tag="ACHKXP_002947"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO7647916.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12321..12803
                     /gene="yfiB"
                     /locus_tag="ACHKXP_002948"
     CDS             12321..12803
                     /gene="yfiB"
                     /locus_tag="ACHKXP_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7647917.1"
                     /translation="MIKHLVAPLVFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWTMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12879..13226)
                     /gene="rplS"
                     /locus_tag="ACHKXP_002949"
     CDS             complement(12879..13226)
                     /gene="rplS"
                     /locus_tag="ACHKXP_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO7647918.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13268..14035)
                     /gene="trmD"
                     /locus_tag="ACHKXP_002950"
     CDS             complement(13268..14035)
                     /gene="trmD"
                     /locus_tag="ACHKXP_002950"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO7647919.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14066..14614)
                     /gene="rimM"
                     /locus_tag="ACHKXP_002951"
     CDS             complement(14066..14614)
                     /gene="rimM"
                     /locus_tag="ACHKXP_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO7647920.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14633..14881)
                     /gene="rpsP"
                     /locus_tag="ACHKXP_002952"
     CDS             complement(14633..14881)
                     /gene="rpsP"
                     /locus_tag="ACHKXP_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO7647921.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15130..16491)
                     /gene="ffh"
                     /locus_tag="ACHKXP_002953"
     CDS             complement(15130..16491)
                     /gene="ffh"
                     /locus_tag="ACHKXP_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO7647922.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16658..17449
                     /gene="ypjD"
                     /locus_tag="ACHKXP_002954"
     CDS             16658..17449
                     /gene="ypjD"
                     /locus_tag="ACHKXP_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO7647923.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17470..18756
                     /gene="yfjD"
                     /locus_tag="ACHKXP_002955"
     CDS             17470..18756
                     /gene="yfjD"
                     /locus_tag="ACHKXP_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO7647924.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18811..19404)
                     /gene="grpE"
                     /locus_tag="ACHKXP_002956"
     CDS             complement(18811..19404)
                     /gene="grpE"
                     /locus_tag="ACHKXP_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO7647925.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19527..20405
                     /gene="nadK"
                     /locus_tag="ACHKXP_002957"
     CDS             19527..20405
                     /gene="nadK"
                     /locus_tag="ACHKXP_002957"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO7647926.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20491..22152
                     /gene="recN"
                     /locus_tag="ACHKXP_002958"
     CDS             20491..22152
                     /gene="recN"
                     /locus_tag="ACHKXP_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO7647927.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22301..22642
                     /gene="bamE"
                     /locus_tag="ACHKXP_002959"
     CDS             22301..22642
                     /gene="bamE"
                     /locus_tag="ACHKXP_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO7647928.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22704..22994)
                     /gene="yfjF"
                     /locus_tag="ACHKXP_002960"
     CDS             complement(22704..22994)
                     /gene="yfjF"
                     /locus_tag="ACHKXP_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO7647929.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22984..23421)
                     /gene="ratA"
                     /locus_tag="ACHKXP_002961"
     CDS             complement(22984..23421)
                     /gene="ratA"
                     /locus_tag="ACHKXP_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO7647930.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23592..24074
                     /gene="smpB"
                     /locus_tag="ACHKXP_002962"
     CDS             23592..24074
                     /gene="smpB"
                     /locus_tag="ACHKXP_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO7647931.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24289..24651
                     /gene="ssrA"
                     /locus_tag="ACHKXP_002963"
     tmRNA           24289..24651
                     /gene="ssrA"
                     /locus_tag="ACHKXP_002963"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24812..29386)
                     /gene="ypjA"
                     /locus_tag="ACHKXP_002964"
     CDS             complement(24812..29386)
                     /gene="ypjA"
                     /locus_tag="ACHKXP_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFO7647932.1"
                     /translation="MNRTSPHYFRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGVQKVDERGTTNNTHIINHGQQEVYGGISNSSIIETGGEQLVSIHADINGQANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVHKGGTSNDTTIKGGTSRIEGGTANGTIIDGGSQ
                     RVTTQGHVDSTTINKSGSQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEKRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTHIYSGGTQIVDNTSTSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNKVADNVLLKNGGHLEVSHSANKTI
                     IKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNINSV
                     TQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETGSA
                     LVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTVDAGGKLI
                     VQKEAKTDTTRLNNGGILEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDAFY
                     IRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSSGTQTIYAS
                     ATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTDKTHINGGTQTVQNYGKAINTD
                     IVSGLQQIMANGTAEDSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQSS
                     QGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNMGG
                     REIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTLTV
                     NDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNMLLENGGNLLVLA
                     GTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGTAI
                     VYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLT
                     AASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTTNE
                     LSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDA
                     SFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPIPNPKPDPKPDPKPDPNP
                     KPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNN
                     VWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGITTLGAFMGYSHSHIGFD
                     RGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFKSNVAGKMSSGGAANGSYHSNGL
                     GGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRELGATLS
                     YNMRLGNGMEVEPWLKAAVRKEFVDDNRVKVNSDGNFVNYLSGRRGIYQAGIKASFSS
                     TLSGHLGVGYSHSAGVESPWNAVAGVNWSF"
     gene            complement(29451..29570)
                     /locus_tag="ACHKXP_002965"
     CDS             complement(29451..29570)
                     /locus_tag="ACHKXP_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7647933.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(29794..29952)
                     /locus_tag="ACHKXP_002966"
                     /pseudo
     CDS             complement(29794..29952)
                     /locus_tag="ACHKXP_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(30298..30896)
                     /locus_tag="ACHKXP_002967"
                     /pseudo
     CDS             complement(30298..30896)
                     /locus_tag="ACHKXP_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7738 a         8124 c         7149 g         7885 t
ORIGIN      
        1 ccgcttcgct ttttctcagc ggcgcggggt gtgcataata caccttcccg ctacagagtc
       61 aagcatttct ttgcttttct ctgttgagat tctcaggaga accccgctga cccggcggcg
      121 tgtttgccgt tgttccgtgt cagtggtggc gcattatagg gagttattcc ggcctgacaa
      181 gcgaaaaata taaaaacttt atcgttcgct cacttttcag gcaaaacatc ttaaatatag
      241 tcttttccgt ctaacttata gacaaaaacg agccccgaag ggctcgtttt atcatttact
      301 ggacggcgac aatccggtct tcattaactt ccaggcgaat cactttaccc ggaaccaatt
      361 caccagacag tatttgctgt gccagcgggt tttcgatctg ctgctgaatt gcacgtttca
      421 gaggacgtgc accatagacc ggatcgtaac cgttctcgct cagcagtttc agcgcctcgt
      481 cagaaatgtg gatttcataa ccacgttctt ccagacgttt gtacagacgt ttcaactgaa
      541 tctgcgcaat cgaggcaatg tgctgttcac ccagcggatg gaagaccacc acttcatcga
      601 tacggttaat gaattccgga cggaagttat ggcttaccac accgagcacc agctctttca
      661 tgtgcgcata atccagttca ccgaagcgtt cctgaatcag atcggaaccg aggttagagg
      721 tcataatgac gaccgtatta cggaagtcga ccgttctccc ttgcccgtca gtcagacgcc
      781 catcatccag tacctgcaac agaatgttga agacatccgg atgcgctttt tccacttcat
      841 ccagcaggat gacggaatac ggacgacgac gcaccgcttc ggtcaggtag ccaccttctt
      901 cataaccgac atatcccgga ggcgcaccaa ccaaacgaga caccgagtgt ttctccataa
      961 actcggacat atcgatacgg accatcgcct cgtcgctatc aaacataaag ttcgccagcg
     1021 ccttacaaag ctctgttttc cccacaccag ttgggccgag gaacaggaat gaaccaatcg
     1081 ggcgatttgg atccgccagc cccgcacggc tacgacgaat agcgttagat accgcatcaa
     1141 ccgcttcgtt ctgaccaatt acgcgatggt gcagttcttg ctccatacgc agcagttttt
     1201 cgcgctcgct ttccatcatg cgagaaaccg gaatccccgt ccaacgcgcc agcacttcag
     1261 caatttcggc gtcggtcact ttattacgca acagacgcat agttttgcct tcgagctgcg
     1321 ttgcggcttc cagttgcttt tccagttccg ggattttgcc gtattgcagt tcagacatcc
     1381 gcgccaggtc ccccacacgg cgagcctgtt caatagcgat tttcgcctgt tccagttccg
     1441 ctttaatggt ctgcgtacca gaaagcgatg ccttctctgc tttccactct tcttctaact
     1501 cggagtactg acgttctttg tcgctcagtt cttcgttgag catatccaga cgttttttac
     1561 tggcttcatc agactctttc attaacgcct gttgttccag tttgagctgg atgatacgac
     1621 gatcgagtcg gtcgagttct tctggttttg agtcaatctg catacgaatg ctggatgctg
     1681 cttcatcgat caggtcgatg gctttatccg gcagctgacg gtcagcaatg tagcgatgag
     1741 acaacgtcgc cgctgcaaca attgccgggt cagtaatttg cacatggtgg tgcaattcgt
     1801 aacgttcttt caggccacgc agaatcgcaa tggtatcttc aacagaaggc tcggcaacaa
     1861 acactttctg gaaacgacgt tccagcgcag catctttttc aatgtactgg cgatattcgt
     1921 caagcgtcgt ggcacctacg cagtgcaatt caccacgcgc cagcgccggt ttcagcatgt
     1981 ttccggcgtc cattgcgcca tcggctttac ccgcgccgac catggtatgt aattcgtcga
     2041 taaataggat gacgttgcct tcctgtttgg caagatcgtt aagcacgcct tttaaacgtt
     2101 cttcaaactc accgcgatat ttcgccccag ccaccagcgc gcccatatcc agcgccagta
     2161 cccggcggcc tttcaaccct tccggcactt cgccgttgat aatacgctgc gccagacctt
     2221 caacgatggc agttttaccg acgccgggtt caccaatcag taccgggtta tttttagtac
     2281 gacgttgcag cacctgaatg gtacggcgaa tttcttcatc acgaccaatc accggatcga
     2341 gtttgccctg ttcggctcgt tcggtaaggt cgatggtata ttttttcaaa gcctgacgtt
     2401 ggtcttcagc accttgatcg ttcacgcttt cacctccacg catttgttca atcgcttgag
     2461 taatgttggc ggtggtcgcc cctgctgctt tcaggatgtc ggccagcgtg ccgcgagact
     2521 caagtgccgc cagaacgaac agttctgacg agataaagtt atcaccacgt ttttgcgcca
     2581 gcttgtcgca aagattaaga acgcgcacca gatcctgtga tggctggaca tcaccaccag
     2641 taccttcaac ctgcggtaaa cgatttaatg cctgattgat atctgtgcgc aactggccag
     2701 catttatgcc agcggatgtt aataaaggac taaccgaacc cccttcctga ttcagcaggg
     2761 cgctcattaa atgaagtggt tcgataaatt ggttgtcgtg cccaagtgca agtgattggg
     2821 catcggcaag agcaagctgg aatttattag taagacgatc cagacgcata actcctccca
     2881 taacggatca aagttgctac tggagattaa atgaggtcat ccctcaatta ttcaaggtta
     2941 ttgaccagat taatgtgaaa agaaaatcac gcgtaccgga tcgtcttgat tctttaggtt
     3001 atatcagcca aatgaaactt gccatacgac cggtggtctt gtcgcgacga taagagaaga
     3061 aagtctcatt ttccgtatat gtacaacggt cgccgccgaa aatttgctca acacccacgt
     3121 tcgccagacg ctgccgggca agctgataaa tatccgccag atacttatca ccatgctgaa
     3181 tgaaagctgc acttgcttta gcgtctactg ccataaacgc ctcgcgaacc tcccccccca
     3241 cttcgaacgc gcgtggacca attgccggcc ctaaccaggc gagaatattt tccggattat
     3301 cagcaaaaca ggaaaccgtc tcttccagca cgcctgcgca cagtccacgc cagccagcat
     3361 gagcggcggc gacttccgtt cccgctcgat tgcaaaacag cacagggagg cagtcggcag
     3421 tcatcactgc gcaaaccgtg ccgggcgtat tgctataaga ggcatccgcc cgttttgagg
     3481 cataaggttc gccagtgagc ttaagcacat ctttgccgtg tacctgctca agccagaccg
     3541 gtttagaagg caaattgccc gcagcaaaaa gtcgcttgcg attctcctca acgtgatccg
     3601 ggttatcgcc acaatgggca ccgaggttga gtgagtcata cgggggcaag ctcacgccgc
     3661 cgatacgagt ggagctacag gccgcaacac cttttggctg cggccactgc gggacaatca
     3721 gcttactcat aaccagtcca cttcatcctt atgttcttcg aaatcggcgc gcatcacctc
     3781 aatcagctcc accatatctt gtggaatagg cgcatgccat tccatttcga tgccggagat
     3841 cgggtgataa agacgcagca tggttgcatg tagcgcctgg cggtcaaact tacgcagcgt
     3901 ggagataaat gcttccgaag caccttttgg cggacgcgga cggccaccat aaaccggatc
     3961 gcccaccagc ggatgagtga tatgggccat atgcacgcgg atctggtgcg tacgtccagt
     4021 ttccagacgc aaccgcagac gcgtgtgcac acggaagtgt tccatgatgc gatagtgagt
     4081 caccgctggt ttgcccatcg gatgcaccgc catatgggta cgtttggtcg ggtggcgact
     4141 gattggctcg tccaccgtgc cacctgcggt catatgacca atcgccaccg cttcatactc
     4201 acgagtaatt tcacgccgtt gcaaagattc gactaaacgc gtctgagccg gaacggtttt
     4261 tgccacaacc atcaggccag tggtgtcttt atccagacga tggacgatgc ccgcacgcgg
     4321 tacatcggca atgggtggat agtaatgaag caacgcattc agtaccgtgc catccgggtt
     4381 acccgcgcca ggatgtacca ccaggtcgcg cggtttatta atgataataa tgtcttcatc
     4441 ttcatagacg atatccagcg ggatatcctg cggttcaaaa cgcgcttctt cttcaatctc
     4501 agcgttgatg gcaacctgct cgccacccaa tactttttct ttcggcttat cacaaacttt
     4561 gccgttaacc agcactcgct ggtcgaggat ccattctttt attcgcgaac gtgaataatc
     4621 cgggaacatt tcggccaaag cctgatctaa gcgttgaccg agttggtttt cggacaccgt
     4681 tgcagtgagc tgtactcgtt gtgccatata tactgcttct tcgtttatcg ttggggtttt
     4741 acggctttgc cgtttaatat agtgtgctat tgtagctggt cttaaccggg agcaggaaca
     4801 gagaatctcc cgtattacat tttgaggaaa gtcaaaacgt catgacgcgc atgaaatatc
     4861 tggtggcagc cgccacacta agcctgtttt tggcgggttg ctcggggtca aaggaagaag
     4921 tacctgataa tccgccaaat gaaatttacg cgactgcaca acaaaagctg caggacggta
     4981 actggagaca ggcaataacg caactggaag cgttagataa tcgctatccg tttggtccgt
     5041 attcgcagca ggtgcagctg gatctcatct acgcctacta taaaaacgcc gatttgccgt
     5101 tagcacaggc tgccatcgat cgttttattc gccttaaccc gacccatccg aatatcgatt
     5161 atgtcatgta catgcgtggc ctgaccaata tggcgctgga tgacagtgcg ctgcaagggt
     5221 tctttggcgt cgatcgtagc gatcgcgatc ctcaacatgc acgagctgcg tttagtgact
     5281 tttccaaact ggtgcgcggc tatccgaaca gtcagtacac caccgatgcc accaaacgtc
     5341 tggtattcct gaaagatcgt ctggcgaaat atgaatactc cgtggccgag tactatacag
     5401 aacgtggcgc atgggttgcc gtcgttaacc gcgtagaagg catgttgcgc gactacccgg
     5461 atacccaggc tacgcgtgat gcgctgccgc tgatggaaaa tgcataccgt cagatgcaga
     5521 tgaatgcgca agctgaaaaa gtagcgaaaa tcatcgccgc aaacagcagc aatacataac
     5581 agaaacctga aacacaaaac ggcagccctt gagctgccgt ttttttattc tgtcagttgt
     5641 gaaactgaag cgatttagtc gctatcgatc tcatcaaata tggctcgctt tgagatattc
     5701 ctcaagtaaa aaaacatctc ttcctgcgat ttctcacaaa aaagattcgt tgacaaaaag
     5761 tgacaaaatt atgagatttt catcacacat tttgacatca ggaacggtat gctgaattca
     5821 ccaagacggg aagacaagag gtaaaattta tgacaatgaa cattaccagc aaacaaatgg
     5881 aaattactcc ggccatccgc caacatgtcg cagaccgtct cgccaaactg gaaaaatggc
     5941 aaacacatct gattaatcca catatcattc tgtccaaaga gccacaaggg tttgttgctg
     6001 acgccacaat caatacacct aacggcgttc tggttgccag tggtaaacat gaagatatgt
     6061 acaccgcaat taacgaattg atcaacaagc tggaacggca gctcaataaa ctgcagcaca
     6121 aaggcgaagc acgtcgtgcc gcaacatcgg tgaaagacgc caacttcgtc gaagaagttg
     6181 aagaagagta gtcctttata ttgagtgtat cgccaacgcg ccttcgggcg cgttttttgt
     6241 tgacagcgtg aaaacagtac gggtactgta ctaaagtcac ttaaggaaac aaacatgaaa
     6301 cacataccgt ttttcttcgc attctttttt accttcccct gaatgggagg cgtttcgtcg
     6361 tgtgaaacag aatgcgaaga cgaacaataa ggcctcccaa atcggggggc cttttttatt
     6421 gataacaaaa aggcaacact atgacatcgg aaaacccgtt actggcgctg cgagagaaaa
     6481 tcagcgcgct ggatgaaaaa ttattagcgt tactggcaga acggcgcgaa ctggccgtcg
     6541 aggtgggaaa agccaaactg ctctcgcatc gcccggtacg tgatattgat cgtgaacgcg
     6601 atttgctgga aagattaatt acgctcggta aagcgcacca tctggacgcc cattacatta
     6661 ctcgcctgtt ccagctcatc attgaagatt ccgtattaac tcagcaggct ttgctccaac
     6721 aacatctcaa taaaattaat ccgcactcag cacgcatcgc ttttctcggc cccaaaggtt
     6781 cttattccca tcttgcggcg cgccagtatg ctgcccgtca ctttgagcaa ttcattgaaa
     6841 gtggctgcgc caaatttgcc gatattttta atcaggtgga aaccggccag gccgactatg
     6901 ccgtcgtacc gattgaaaat accagctccg gtgccataaa cgacgtttac gatctgctgc
     6961 aacataccag cttgtcgatt gttggcgaga tgacgttaac tatcgaccat tgtttgttgg
     7021 tctccgacac tactgattta tccaccatca atacggtcta cagccatccg cagccattcc
     7081 agcaatgcag caaattcctt aatcgttatc cgcactggaa gattgaatat accgaaagta
     7141 cgtctgcggc aatggaaaag gttgcacagg caaaatcacc gcatgttgct gcgttgggaa
     7201 gcgaagctgg cggcactttg tacggtttgc aggtactgga gcgtattgaa gcaaatcagc
     7261 gacaaaactt cacccgattt gtggtgttgg cgcgtaaagc cattaacgtg tctgatcagg
     7321 ttccggcgaa aaccacgttg ttaatggcga ccgggcaaca agccggtgcg ctggttgaag
     7381 cgttgctggt actgcgcaac cacaatctga ttatgacccg tctggaatca cgcccgattc
     7441 acggtaatcc atgggaagag atgttctatc tggatattca ggccaatctt gaatcagcgg
     7501 aaatgcaaaa agcattgaaa gagttagggg aaatcacccg ttcaatgaag gtattgggct
     7561 gttacccaag tgagaacgta gtgcctgttg atccaacctg atgaaaaggt gccggatgat
     7621 gtgaatcatc cggcactgga ttattactgg cgattgtcat tcgcctgacg caataacacg
     7681 cggctttcac tctgaaaacg ctgtgcgtaa tcgccgaacc agtgctccac cttgcggaaa
     7741 ctgtcaataa acgcctgctt atcgccctgc tccagcaact caatcgcctc gccgaaacgc
     7801 ttatagtaac gtttgattaa cgccagatta cgctctgacg acataatgat gtcggcataa
     7861 agctgcggat cctgagcaaa cagtcgcccg accatcgcca gctcaaggcg gtaaatcggc
     7921 gaagagagcg ccagaagttg ctcaagctga acattttctt ctgccaggtg cagcccgtaa
     7981 gcaaaagtag caaagtggcg cagtgcctga ataaacgcca tattctgatc gtgctcgacg
     8041 gcgctaatac gatgcagccg agcgccccag acctgaattt gctccagaaa ccattggtat
     8101 gcttccggtt tacgtccatc acaccagacc acaacttgct ttgccaggct accgctgtcc
     8161 ggaccgaaca tcgggtgtag ccccagcacc ggaccatcat gcgccaccag catggcctgt
     8221 aatggcccat ttttcactga tgccagatcg accagaatac aatctttcgg taaaggcggt
     8281 aatttgccaa taacttgctc agtaacgtgg attggcacac taacaatcac cattccggca
     8341 tcggcaacaa tatcagccgc tcgatcccag tcatgttgct ccagaatccg cacctgataa
     8401 cccgagaggg tcagcatctt ctcgaacagg cgtcccatct gaccgccacc gccgacgata
     8461 accaccggac gcagtgacgg acaaagtgtt ttaaatcctt tgtcgttttc actggagtaa
     8521 gattcacgca tcacccgacg caaaacatcc tcaatcagat ctggcggtac acccagagct
     8581 tccgcctctg cacgacgcga ggccaacata gatgcctcgc gctccggaac ataaataggc
     8641 agtccaaagc ggcttttcac ctcgcccact tcagcaacca gttccagacg cttcgctaat
     8701 aaattcagca gcgctttatc gacttcatca atttgatcgc gtaatgcggt caattcagca
     8761 accataataa acctcttaag ccacgcgagc cgtcagctgc ccgttcagat cctgatgaat
     8821 ttcacgcagc aaggcatcgg tcatttccca gctaatgcag gcatcggtta cggatacacc
     8881 gtatttcatt tcactgcgcg gttgctcgga agactgattg ccctcgtgga tattactttc
     8941 gatcatcaga ccaataattg agcgattgcc atctttgatt tgagcaacca cggattctgc
     9001 caccgcaggc tgacggcgat aatctttatt ggaattaccg tggctgcaat ctaccatcag
     9061 agacgggcgc agtcccgcct gttccatctc tttttcacat tgcgcaacat ccgcagggct
     9121 atagttcggc gctttaccac cgcgcaggat cacatggccg tccggattcc cctgagtttg
     9181 tagcaacgca acctgccctg cctggttaat gccaacaaaa cggtgcggct gggcggcggc
     9241 gcgcatagcg ttaattgctg ttgccagact gccgtcggtg ccgtttttaa aaccaaccgg
     9301 catggaaagc ccggaggcca tttcacggtg agtttgcgat tccgttgtac gagcaccaat
     9361 tgctgaccag ctaaacagat cgcccaggta ttgcgggcta ttcggatcta acgcttccgt
     9421 cgccagtggc agtcccatat tcaccagctc aagcagcaat ttacgcgcga tctgcagccc
     9481 ggcttctaca tcaaaagagc catccatatg gggatcgtta attaaccctt tccagccgac
     9541 agtggtacgg ggtttttcaa aatagacgcg cattaccaga tagaggctat cgctgacctc
     9601 tgcggcaagg gctttaaatc gacgagcata ttccagagca gtttccggat catgaatgga
     9661 acaaggacca catactacca gcagacgagg atcgcgcccg gcgataatat ctgaaatgct
     9721 tttacgcgag tcagcaatct gggcttcttg ttgcaggctc aatggaaaag cggccttcag
     9781 ttgttccgga gtcattaaaa cctgttcgtc ggtaatatgt acgttattca gcgcgtcttt
     9841 ttgcatgatg gcgatcctgt ttatgctcgt ttgcgatagt tgatcctcag cgaggatgac
     9901 gtaacgataa cacataaagt aaagttttca atccatattt cgtacatttt tatttacaca
     9961 ggcaatttag tcgcgctttc aacccttacc tctgtataga taaatttaca ctccctttga
    10021 aaacaatccg ctatgctttg aaaaaggaga aagaaatgat gaaaaagttt atcgccccct
    10081 tgttggcttt actggttagc ggatgtcaga ttgatcctta tactcacgcg ccaaccttga
    10141 ccagcaccga ctggtatgat gtcggtatgg aagatgcgat atcgggcagc gccataaaag
    10201 atgacgatgc atttagcgat tcacaggcgg atcgcggtct ataccttaaa ggatatgccg
    10261 aaggacaaaa gaaaacttgc cagaccgatt ttacttatgc ccgaggactt tccggtaaaa
    10321 gctttcctgc cagctgcaac aatgttgaaa atgccagcca actgcatgaa gtatggcaaa
    10381 aaggggctga tgaaaatgcc agcacaatac gattaaatta attaaaaccc cacaaataaa
    10441 ttaaacataa gattttactc attgtgtttt atttctcaca ttgatgacgg tcgcatgaaa
    10501 aaatgatatt ttaatgagca ttctgaaaaa atatcgatgc atttcgagcg aagatggtgg
    10561 ggatccctga atgcgttttt ctcaccgact gttcttactt ctgattctcc tgctgacggg
    10621 agcaccaata ctcgctcagg agccttctga cgttgcgaaa aacgtgcgta tgatggtttc
    10681 cggtattgtc agttacaccc gctggcctgc gctatcaggg ccaccaaagt tatgtatctt
    10741 ttcatcttca cgttttagta ccgccctaca agagaatgcc gcgacatctt tgccttatct
    10801 gcctgtcatc attcacactc aacaagaggc gatgatttca ggctgcaatg gtttttattt
    10861 tggcaacgag tcaccgacat tccagatgga attaaccgaa caatatccgt caaaggcgtt
    10921 attattaatt gccgaacaaa ataccgagtg cattattggt agcgcctttt gtctgatcat
    10981 ccacaataat gacgtcagat ttgccgtaaa cctggatgcc ttatcgcgta gcggtgtaaa
    11041 ggtcaacccg gatgtcttaa tgctcgcacg gaagaaaaat gatggataac gataattctc
    11101 ttaataagcg ccccacgttt aaaagagcat tacgcaacat cagtatgacc agcatattta
    11161 tcactatgat gctgatctgg ttgctgcttt ccgtgacctc ggtgctgacc ctgaaacagt
    11221 acgcgcaaaa aaacctggca ctgacagcag caacaatgac ttacagtctg gaagcagctg
    11281 tcgtttttgc cgatggccct gcagcaactg aaacactggc agcgctgggc cagcaagggc
    11341 aattttcaac tgcagaagta cgtgataagc agcaaaatat tctggcatcc tggcattaca
    11401 cccgtaagga tccaggcgat actttcagta atttcataag ccactggctc ttccctgccc
    11461 ccatcattca gccgattcgt cacaatggtg aaaccattgg cgaagtacgc ttaaccgctc
    11521 gcgacagttc aatcagccat tttatctggt tttcgctcgc cgtactgacc ggttgtattc
    11581 tgctggcatc aggcatcgca attaccctca cccgccattt gcacaatggc ctggtagaag
    11641 cgctgaaaaa tatcaccgat gtcgtacatg atgtgcgttc caaccgcaat ttttcccgac
    11701 gagtttcgga agaacgtatc gctgagtttc accgcttcgc tctcgacttc aacagtctgc
    11761 tggatgaaat ggaagagtgg cagcttcgtt tacaggctaa aaatgcgcag cttctacgta
    11821 ccgcgctaca tgacccatta accgggctgg ctaaccgcgc agcgtttcgt agcggcatca
    11881 acacgttgat gaacaattcc gatgcccgaa aaacgtcggc gttactattt cttgatggcg
    11941 ataatttcaa atacatcaat gatacctggg gtcatgcgac gggcgataga gtcttgattg
    12001 aaatcgcaaa acggttagct gaatttggcg ggctgcgaca taaagcatac cgcctgggcg
    12061 gcgatgaatt cgctatggtg ctctatgatg tacagtcgga atctgaagtg cagcagatat
    12121 gttcagcact gacacaaatc tttaatctcc cgtttgatct tcataatggc catcagacca
    12181 ccatgacatt aagcattggt tacgcgatga ccattgagca cgcctctgcg gaaaaattac
    12241 aagagcttgc cgatcacaat atgtatcagg ccaaacacca gcgtgccgaa aagctggtga
    12301 gataacaagg atatatcgat atgataaagc acctggtagc acccctggtt ttcacctcac
    12361 taatactgac tggctgccag tcccctcagg gaaagtttac tcctgagcaa gtcgccgcta
    12421 tgcaatctta tggatttact gaatccgccg gcgactggtc gctgggctta tcagatgcca
    12481 ttctgttcgc aaaaaatgac tacaaattgc tcccggaaag ccagcaacag atccaaacca
    12541 tggcagctaa attggcctcg acagggctaa cacatgcccg tatggatgga cacaccgata
    12601 actatggtga agacagttac aacgaaggct tatcattgaa acgggcgaat gtcgtggccg
    12661 atgcatggac tatgggtgga caaattccac gcagcaatct caccacacag ggtttaggaa
    12721 aaaaatatcc catagccagt aacaagaccg cccagggccg cgccgagaac cgccgcgtcg
    12781 cagtggtgat tactacccct taaaacacca gcaaacagat aaaaaagggc cagccaattg
    12841 gccagccctt cttaacagga tgtcgcttaa gcgaaatctt agttaagacg ctctttgata
    12901 cgagcagcct taccagtacg ctcacgcagg tagtacagtt tagctttacg aacagcacca
    12961 cgacgtttga cagaaatgct gtcaactacc ggagagtgag tctggaagac acgctcaacg
    13021 ccttcgccgt tggaaatttt acgaacagtg aatgcagagt gcagaccgcg gttacgaata
    13081 gcgataacca cgccctcgaa tgcctgcaga cgttttttgg aaccttcaac aacccatact
    13141 ttcacttcca cggtatcacc cggacggaag gaaggtacgt cctgcttcat ctgctcttgt
    13201 tcaagttgct taataatgtt gctcataatt taatctctta tcctgggtaa actgatatct
    13261 cgggggctta cgccatccca tcatgtttat gttgctgttg tgcgtgttcc gttttgaact
    13321 ccgccagcaa ccttgcttgc tcttcagtca gagccaggtt ttccagaagt tcaggtcttc
    13381 taagccaggt acggcccagc gactgtttca aacgccagcg acgtatctcg gcatggttgc
    13441 ccgacagtaa cactggcgga acttccatcc cttctaacac ctcaggccgc gtatagtgcg
    13501 ggcaatccag caatccttca gcaaaggaat cttccgttgc cgaggcttca tgtcccagta
    13561 ctcccggaat aaaccgggaa acggagtcaa tcagcgtcat tgctggtaac tcaccaccac
    13621 tgagaacgta atcgccgatt gaccattctt cgtcaatttc ggtttggatc acgcgctcat
    13681 ctataccttc gtagcgaccg cacaccagaa tcaatttttg attcgttgcc agttcgctga
    13741 cgcccgcttg atcaagcttg cgtccctgtg gtgacagata aatcaccttt gcgccttcac
    13801 ccgccgcggc ttttgctgca tgaatggcgt cccgcaaggg ttgcaccatc attaacatcc
    13861 ccggtccgcc gccgtaagga cgatcgtcca cggtacggtg ccggtcatgc gtgaagtcgc
    13921 gaggactcca gctctggatg ctcagcaggc catttttaac tgcccggcca gttaccccgt
    13981 aatcggtaat tgcgcggaac atttcaggaa acaggctaat tatgccaatc cacatagcgc
    14041 cgtcttttac cgtttatccg gtggtttaaa aaccaggatc ccaatctact tcgattgaac
    14101 gagtagtgag atcgactttc ttgataacct gcccatcgag gaacggtacg agacgttcct
    14161 tgataccaaa cgcatctttc aggtttgcct taatgacgag aacgtcatta gatccggttt
    14221 ccatcatatc gacgacttta ccgagatcgt agccttcagt ggttactacc tggcagccca
    14281 tcaggtcttt ccagtagtag tccccctctt caagctgagg cagctgcgat gaatccacga
    14341 caatttcaca attcgtcagc aggttcgccg catcacgatc gtcaacgcct ttcagcttga
    14401 tgatcatgtc ctgattgtgg tgcttccagc tttccagctg gacttgctgc cactgacccg
    14461 ccttctggat aaaccagggc tgatagtcaa aaatgctttc ggcgtcttcg gtggaagaaa
    14521 acactctgag ccacccacga ataccgtaag acgaacccat ttttcccaaa acgatgggat
    14581 caacaggtgc ttgcgcggtg agttgtttgc tcatcatgac caccgtgaca gattaagctg
    14641 ctttgtttac ttctttgatc agcgcagcaa cgcgatcaga aatagttgcg ccctggccaa
    14701 cccagtgagc gatgcgatcc agatccaggc gagtgccttc ttctttttcg ctagcgattg
    14761 ggttgaagaa accaacgcgc tcgatgaagc gaccgttgcg tgcattacgg ctgtcagcga
    14821 caacaacctg gtagaacgga cgctttttag cgccgtgacg tgctaaacga atagttacca
    14881 taacatcctc ttgtgtgaat aaaacaaccg gaccccatcg aggaacggag tccggtgtca
    14941 tattaaaagc ccgaaaattt tactcatttt tgcgggaatt gcaatcaaca gttgctaact
    15001 ctgctgtaaa aggccgtcgg cggtgcagcc agtttggtgc cggagtgcgc gcagtcaccg
    15061 gagcgtacac gcagtacgtg aggatgacga gcacatcccg gtgccaaaat ggcaaacaag
    15121 ccaggccgat tagcgaccag ggaagcctgg gggcatcata cccttcatgc ttctcatcat
    15181 cttcgccatt ccgcccttct tcattttctt catcatgcgc tgcatgtcgt cgaactgttt
    15241 cagaagacgg ttaacgtcct gcacctgcat accgcaaccg gcagcaatac ggcgtttacg
    15301 cgaacctttg atgatttctg gcttagcgcg ctctttcatc gtcatcgagt tgatgatggc
    15361 ttccatacgc accagcactt tatcgtccat ctgtgacttg acgttatccg ggatctgccc
    15421 catgcccggc agcttgccca tcagactagc catgccgccc atatttttca tctggcgcag
    15481 ctgctcaaga aagtcgttga gatcgaagcc gtcacctttt ttcagcttgc tggctaattt
    15541 ctctgcctgc gcgcggtcaa ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc
    15601 catgccgaga atacgcgacg cgatgcggtc cggatggaac ggctccagcg cctcagtttt
    15661 ctcgccaaca ccgaggaact tgatcggttt gccagtgatg tgacgaatag agagcgccgc
    15721 accgccgcgg gcatcgccgt ccactttggt caacactacg ccggtaagcg gtaacgcttc
    15781 attgaatgct tttgccgtat tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag
    15841 ggtttcaacc gggttaatcg acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc
    15901 aacgtgcaga cgaccagcgg tatccaccag cagcacgtcg tagaatttca gtttggcttc
    15961 tttcagcgcc gcgttaacga tatctaccgg cttctgacca acatcagaag ggaagaaatc
    16021 aacgcccacc tgctctgcca gcgtctcaag ctgtttgatt gccgccgggc gataaacgtc
    16081 ggcagaaacc accagcactt tcttcttgtg cttctcgcgc aggaacttac cgagtttacc
    16141 aacgctggtt gttttaccgg caccttgcag gcccgccatc agtacgaccg caggcggttg
    16201 cgcagccagg ttcagggtct ggttctcttc gcccatcgcc gcaaccagtt cgttacggac
    16261 tattttgacg aactcctgcc ccggcgtcag gctcttatta acttcatgac caaccgcttt
    16321 ctcttttacg cgattgataa actcacgcac taccggcaga gctacgtccg cctccagcag
    16381 cgccatgcgc acttcgcgca gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc
    16441 actgatattg cgcagcgtgc gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg
    16501 cctggggtgg aaacggttgg ccgcaatcgc gacacatcat cagtattttg ccgcagtata
    16561 acatgaaggc gtctttgttg ttatgcaacg gttggagcag cgttcacctg acgctatact
    16621 gcttctcttt cttattgctc aaactgtcga catcactatg cccgtttttg ctctgctcgc
    16681 gcttgtcgcc tactccgtca gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg
    16741 cggctggcgg cgcatggcta ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc
    16801 tctggaagcc cgcatcctgc ccgacggtga tagcggacaa aacctcagcc tgctgaacgt
    16861 tggttcattg gtcagtttga tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg
    16921 tggctggctg ctgctaccca ttgtctatgc ctttgcgctt atcaacctgg cgctggcaac
    16981 cttcatgccc aatgaataca tcacccatct ggaagctacg cctgggatgc tggtgcacat
    17041 tggcttatcg ctcttttcct atgccacgct aattatcgcc gccctgtacg cgctgcaact
    17101 ggcgtggatt gattaccaac tgaagaacaa gaagctggcg tttaaccagg aaatgccgcc
    17161 attgatgagt atcgagcgta aaatgttcca catcacgcag attggcgtgg tgctgctaac
    17221 gctcacgctt tgcactggcc tgttctacat gcacaactta tttagcatgg aaaatatcga
    17281 caaggctgtg ctctctatcg tggcgtggtt tgtctatatt gtgctgctgt ggggacatta
    17341 tcatgaagga tggcgtggac gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct
    17401 gacactggcc tacttcggca gccgaattgt ccagcagtta atcagctaaa cccagaaaag
    17461 gagtttcccc tggaacacat ttctactact acgttgatca ttattctgat catcatggtg
    17521 gtcatttcag cctatttttc cgggtccgaa accggaatga tgaccctcaa ccgctatcgt
    17581 ctgcgacata tggcgaaaca gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt
    17641 aagccagacc gcctgataag cctggtgtta atcggcaata acctggtcaa tattcttgcc
    17701 tctgcgctcg gcactattgt tgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg
    17761 actggtgtgc tgacttttgt cgtactggta tttgctgagg tattgccgaa aaccattgcc
    17821 gcgctgtacc cggaaaaagt cgcttatccg agtagttttc tgctggctcc gctgcaaatt
    17881 ttgatgatgc cgctggtctg gttgctgaat gctatcaccc gtatgctgat gcgcatgatg
    17941 ggtatcaaaa ccgatatcgt ggttagcggc tctttgagca aagaagagtt gcgcactatc
    18001 gtgcacgaat cacgctcaca aatttcccgt cgcaatcagg atatgctgct gtcggtgctc
    18061 gatctggaaa aaatgaccgt tgatgacatc atggtgccgc gcagtgaaat tatcggtatt
    18121 gatatcaacg atgactggaa atcgattctg cgccaactct cccactcacc tcacgggcgc
    18181 atcgtgctct accgtgattc gctggacgac gccatcagta tgctgcgagt acgtgaagcc
    18241 tggcggttga tgtcggagaa aaaagagttc accaaagaaa ccatgctgcg cgccgcggac
    18301 gagatctatt ttgtgccgga aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc
    18361 aacaaaaaga aagtcggcct ggtcgtcaac gagtatggag acattcaggg gctggtgacg
    18421 gttgaagata ttctggaaga gattgtcggc gatttcacta cgtcgatgtc gccaacactt
    18481 gccgaagagg tcacgccgca aaacgacggt tcggtgatta tcgatggcac cgccaacgtg
    18541 cgggaaatca acaaagcctt taactggcat ctaccggaag atgatgcccg cacggttaat
    18601 ggcgtcattc ttgaggcact ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc
    18661 gagtacgata tcgatattct cgacgtacag gacaatatga ttaagcaggt aaaagttttt
    18721 cctgtgaaac cgctgcgcga gagtgtggcg gagtaacgaa aacggcccgg cattcgcatg
    18781 cagggccgtg aattattacg aaagcagaaa ttaagctttt gctttcgcta cagtaaccat
    18841 cgccgcacga atcgtacgac cattcagcgt ataacccttc tgcataatgc ccagtacgtt
    18901 acctggcgca acgtcatcag attccaccat tgcgatggcc tgatgcacat tcgggtccag
    18961 tgggacgtta gtttcggcga tcacttcaac gccaaactta cgcacaacat ccagcatcga
    19021 cttcagcgtc agctcaatgc cttcaaccat cgcagacata tccgggttag ctttatcagc
    19081 cacttccagc gcacgatcca ggctatcaat caccggcagc aattcgttga tgaatttctc
    19141 cagcgcgaat ttgtgggctt tttcaatatc cagttcagta cgacgacgca ggttttccat
    19201 ttcggctttt acacgcaaaa tgccgtcacg ttcacgggtc tgggcttcag ccagctgagc
    19261 ttcgagattc gcaatttttt catcgcgcgg atccacctgc tcagcagaag cttctggctc
    19321 aactgcctca atctcttcgt gctgatccat gataatttct tccggggctt gcccctcagg
    19381 cgttttctgt tctttactac tcatgaattt ctccgcgttt ttttcgcatt catctcgcta
    19441 acttcgctta ttatggggat cagtttcagg gtttcaaggg aagcactcac attgtcatca
    19501 atcttcgcaa caaggacctc ggaaaaatga ataatcattt caagtgtatt ggcattgtgg
    19561 gacacccacg gcaccccact gcactgacaa cacatgaaat gctctaccgc tggctgtgca
    19621 caaaaggtta cgaggtcatc gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg
    19681 tgaaaactgg cacgctcgcg gagattgggc aactagctga tctcgcggta gtcgttggtg
    19741 gcgacggtaa tatgctgggc gcggcacgca cactcgcccg ttacgatatt aaagttattg
    19801 gaatcaaccg tggcaacctg ggtttcctga ctgaccttga ccccgataac gcccagcaac
    19861 agttagccga tgtgctggaa ggccactaca tcagcgagaa acgttttttg ctggaagcgc
    19921 aagtctgtca gcaagattgc cagaaacgca tcagcaccgc gataaatgaa gtggtgcttc
    19981 atccaggcaa agtggcgcat atgattgagt tcgaagtgta tatcgacgag atctttgcgt
    20041 tttctcagcg atctgatgga ctaattattt cgacgccaac aggctccacc gcctattccc
    20101 tctctgcagg cggtcctatt ctgaccccct ctctggatgc gattaccctg gtgcccatgt
    20161 tcccgcatac gttgtcagca cgaccactgg tcataaacag cagcagcacg atccgtctgc
    20221 gtttttcgca tcgccgtaac gacctggaaa tcagttgcga cagccagata gcactgccga
    20281 ttcaggaagg tgaagatgtc ctgattcgtc gctgtgatta ccatctgaat ctgattcatc
    20341 cgaaagatta cagttatttc aacacattaa gcaccaagct cggctggtca aaaaaattat
    20401 tctaatttta cgccagcctc tttactgtat ataaaaccag tttatactgt acacaataac
    20461 agtaatggtt tttcatacag gaaaacgact atgttggcac aactgaccat cagcaacttt
    20521 gctatcgttc gtgagcttga gattgatttt catagcggca tgaccgtaat aactggcgag
    20581 accggcgcgg gtaaatctat tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct
    20641 gaagccgaca tggtgcgtac cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg
    20701 aaagatacgc cagcggctct gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa
    20761 tgtttgcttc gtcgcgtgat cagcagcgat ggtcgctccc gtggtttcat caacggtaca
    20821 gctgttcctc tgtcacaact gcgcgaactg ggtcagttgc tgattcagat ccatggtcag
    20881 cacgctcatc aattactcac caaacctgag caccaaaaat tcctgcttga tggctatgcc
    20941 aatgaaacct ctctactgca ggaaatgacc gcacgttatc agttgtggca tcaaagctgc
    21001 cgtgacctcg cgcatcatca acagttaagt caggaacgcg ccgcccgtgc ggaactgctg
    21061 caataccaat taaaagaact taacgaattt aatccgcagc ccggagagtt tgaacaaatc
    21121 gacgaagagt acaaacgtct ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca
    21181 ttggcattaa tggccgacgg tgaagacgca aacctgcaaa gtcagcttta cacggctaaa
    21241 caactggtga gcgaattgat tggcatggac agcaaactgt ccggcgtact tgatatgctg
    21301 gaagaagcta ccatccagat tgctgaagcc agcgatgaac tgcgccacta ctgcgatcgt
    21361 ctggatctcg atcccaaccg actatttgaa cttgaacagc gcatctcaaa acagatttcg
    21421 ctggcacgta aacatcacgt cagccctgag gcattgccac agtattacca gtcgctactg
    21481 gaagaacagc agcaactgga cgatcaggcc gactcacaag aaacgcttgc gctggcggta
    21541 acgaaacatc atcagcaggc actggaaatc gcgcgcgcat tacaccaaca acgccagcaa
    21601 tatgcagaag aacttgcaca gctgatcacc gacagtatgc atgcgctctc aatgccgcat
    21661 gggcagttta cgatcgatgt taaatttgac gagcatcacc tgggcgctga cggtgccgat
    21721 cgtattgagt ttcgggtaac caccaaccca ggtcagccaa tgcagcctat tgccaaagtc
    21781 gcatccggtg gtgaattgtc ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa
    21841 atggaaaccc cggcactgat ttttgatgaa gtggatgtag ggattagcgg tccaacagcg
    21901 gcagttgtcg gcaaactgct gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc
    21961 cacctgccac aagtcgcggg atgtggtcat caacactatt ttgtcagcaa agaaaccgat
    22021 ggtgcgatga cagaaacgca tatgcaatcc ctgaataaaa aagcgcggtt acaagagctg
    22081 gcgcgcctgc ttggtggcag tgaagtcaca cgtaatacac tggcgaatgc gaaagaactg
    22141 cttgcagcgt aaactttttt cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga
    22201 ccggaaagca aaaggtttta aagtgatgaa aggtctatta tcatcggcat attacagatg
    22261 agccacgtac tgctcgggcc cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac
    22321 tgctgcagca gcagtactat tgatgttgac cgcaggctgt tccactctgg agcgagtggt
    22381 ttaccgtcct gacatcaacc aggggaacta tctgaccgct aacgacgtat ccaaaatacg
    22441 tgttggcatg acgcaacaac aagttgcgta cgcattgggt acaccgctga tgtccgatcc
    22501 atttggtacg aatacctggt tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac
    22561 tcagcaaacg ctgacgctga cctttaacag tagcggtgtg ttgaccaata ttgataacaa
    22621 acctgcgctg agtggtaact aataataaag ttgctctcaa agacgttaaa aaaggtgctc
    22681 aatgagcacc ttttttctgt ctgttattta ttcgctgatt tttctgctcg ttgcctgcga
    22741 agctctttcg gatcggcaat gagagggcga taaatctcca cccgatcgcc atcatgcaca
    22801 ctatcgctta gttttgccgg acggctgtaa atgccgactt tgtttttagt taaatcgata
    22861 tcggtacgca attccagcaa gccactggcg cgaatagctt cttcaaccgt cgcgccctcc
    22921 tgcagcgtca ctcgctgcag gtactgcttc tcaggtagcg cataagccac ctcaacggca
    22981 attttacctg gcactgtaaa cctctttcgc acgaaccgta aaagcctgga ccatattagc
    23041 cgccagctct ttaaacacgc gaccaaaggc gagttcaatc aacttattgg taaactcaaa
    23101 gtcgagatga aactcgatac gacacgcctc ctggctcagc ggcgtaaact tccatccacc
    23161 aatcaatttc ttgaacggcc catccaccag attcataaga atactttggt tactggtcaa
    23221 ctggttgcgg gtagtaaacg ttttgctgat cccagcctta gagacatcta ccgccgcagt
    23281 catctgccca ggagtggact ccagaatccg acttccggta caacccggca aaaactgagg
    23341 ataagactga acgtcattca ctaactgata catttgctcc gcgctgtagg gtaccagtgc
    23401 ggtccggcta atctgaggca taacaatttc catcaacaaa aatccaacaa ataatatcat
    23461 ttatcctgct gtaaaaaaaa cgctatcccg gcgctgggta acatcgggtt catgctaaga
    23521 tagagccttg tcccccgcag gattgatatg gggtgttttc gatttcagat taccgatgat
    23581 tcacgacgct tatgacgaag aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta
    23641 acaagcgcgc ccgtcacgaa tactttatcg aagaagagtt cgaagcggga cttgccctgc
    23701 aaggctggga agttaaatcc ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc
    23761 ttctgcgtga cggagaggca tttctgtttg gcgctaacat cacgccaatg gccgtggcct
    23821 ccacgcatgt ggtgtgcgat cctacccgta cccgcaagtt acttctcaac cagcgcgaac
    23881 tggactcatt gtacggtcgc gtcaatcgag aaggctatac cgtagtggcg ctctccctgt
    23941 actggaaaaa tgcctggtgc aaagtgaaaa tcggcgtcgc caaaggtaag aaacagcacg
    24001 ataaacgttc agatatcaaa gagcgcgaat ggcaggtgga taaagcacgt atcatgaaaa
    24061 acgcccaccg ttaaacctgc actccaatta ttgaccagtt cctcaccgcg cctccctctc
    24121 cggcggcgcg aatgaacatc ttattggcta tcacatccga cacaaatgtt gccatcccat
    24181 tgcttaatcg aataaaaatc aggctacatg ggtgctaaat ctttaacgat aacgccattg
    24241 aggctggtca tggcgctcat aaatctggta tacttacctt tacacattgg ggctgattct
    24301 ggattcgacg ggatttgcga aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa
    24361 agccgcaaaa aatagtcgca aacgacgaaa actacgcttt agcagcttaa taacctgctt
    24421 agagccctct ctccctagcc tccgctctta ggacggggat caagagaggt caaacccaaa
    24481 agagatcgcg tggaagccct gcctggggtt gaagcgttaa aacttaatca ggctagtttg
    24541 ttagtggcgt gtccgtccgc agctggcaag cgaatgtaaa gactgactaa gcatgtagta
    24601 ccgaggatgt aggaatttcg gacgcgggtt caactcccgc cagctccacc aatcatgatt
    24661 ggacggtgta aggacaacac caacaaaaac aggaagttag aagtctcagc aaaacaccga
    24721 ccagacggtg aggagacata aaaggatacg caaaggagcc gcggctcctg gtaacatgaa
    24781 agcccacaga tgtgggcttt ttcgttgatg gtcagaacga ccagttcaca ccagctaccg
    24841 cgttccacgg ggattccaca ccggcactat ggctataccc caccccaaga tgcccgctta
    24901 acgtactgct gaatgaggct ttaatacctg cctggtatat tccacgtctg cccgacaaat
    24961 aattgacgaa attaccgtca ctattcactt tcacccggtt atcatcgaca aattctttgc
    25021 gcacagccgc cttcagccac ggctcaactt ccataccgtt ccccagacgc atgttgtaac
    25081 tcagcgttgc gcccagttca cgatatatac tgcgggtatc gactgatttc gatttcatgc
    25141 cattggataa atgatattcg gggttatcag cggtgaaccc cgttaacgat gcatacggcg
    25201 tcaggttcca gttaccatcg gtaaatcgca tcccggtttc aatgtgaccg cccagcccgt
    25261 tgctgtggta actgccattg gcggctccac cgctgctcat tttacctgct acgttacttt
    25321 taaaacggtt cagcttcacg acaccgtcca gatagaaacc actttcatgt tcccaactgg
    25381 catagccgcc cagagaataa ctgcccacac tgccatgtcc tccgcgatca aaaccgatat
    25441 gtgaatggga atagcccata aaagcgccta gcgtggtaat tccttcagga atatcattac
    25501 ggctgtcgat ccccactgtc attccggtca gcgtctgctc aaacccggca cccgcatcgg
    25561 tggtgacatt attacgggtg ttatacgtcg ccccccagac attattgttg tgtggactcg
    25621 ctttcattat gttcaaccgc tcgcgaatac tgtttagctc agcatcaaat accaacggta
    25681 atgttgctgc catattgagt acggctgccg tagaaggcgt aatgcgtttc tccggaacgg
    25741 gtgtcggcgt tggatcggga gtagggtctg gttttggatt tgggtctggc tttggatcgg
    25801 gttttgggtc tggctttgga tttgggatgg ggtccgggtt gggtttgaca tcattggtca
    25861 ggttccagtt gctgttgccg tcacttttca ggacatactc ataggtccca agatcaacga
    25921 aaccgccggt attgcccagc gtaaacgaag catcccctcc ccctgttttc accagcgtca
    25981 tcgcgtcgtc agactgtggg ctgacgccgg tatcctgaac aaagatttta aaattaccag
    26041 tggcgttgtt gttgacgacc agttgatcgc cccgggagcc tgcaacgttg gtatgcaggt
    26101 agaaattacc gctaccggaa agttcattgg ttgtcagcgt attgtagata ccgttagttg
    26161 tggcaggcgc tgctgtttgt gctgacaaat aaacatcacc gtcgttgagt agcaaactgt
    26221 ttactctata ttcgcagttg ctggtacctg cacaggaatt attgctgtta agccagacac
    26281 tgccccggct ggcagccgtc aggtcggcaa gcgtggtatc gacgccattt cccagagtca
    26341 atgtcgcgct atcggtaatc cggaccgccc cttcgagttt aactggcgta acattatccc
    26401 gtggcgtcat taacgacagg cttcctgtcg cgccactgac cgatgcatcc gccagcgtac
    26461 ctgcgtagac gattgccgta ccgccagcga cctggagatc ttctgcccgg gccagagcct
    26521 gaaactcatc ttttgaccgc ccaagggtat attgcccgcc agagttaacc tttgtggcgg
    26581 agtcctgacc caggttttgc attgccccac ccttgccaac cgtggagtcg cgagcttcgg
    26641 tacctgctaa taccaataaa ttaccgccat tttccagcaa catattggtc gctaaattgc
    26701 cggaaatgga aaaagtaccg tactggtgag taccgctgat ttcaataccg ttagccgtgc
    26761 tcgtctggag agcggcaccg ctgttctgga cgatatctgt cgctttgcca ttatcgttga
    26821 ctgtcagcgt accgccttca ttgatctttg tttttattgc ctctccgtta gctgaaactg
    26881 tttgtattcc gccgtcgtta attgttgtct cattcgccac accctcgaca atttgttcac
    26941 cgccggtgag cgtcgtgcct gtcgcagtgg cttttgtttt gacgatctcc cgtccgccca
    27001 tattgacctg tgttttgtca gaagaggtgt ctgactccac ggttaacacg ccgccatttg
    27061 ccagcaggat attgttcgcc gcaccctgct cgatgctgaa cgcgacgcca tccgcgcgtg
    27121 ttcctgtgac ccgcgtcgcc ctggtggttg caaccaaagc gccctggcta ctctgctgta
    27181 tccccgttgc gctgcctttc tcccgcacat cgagtgtgcc gccgtcatta agcaccgagt
    27241 tttcagccag accgccctca ttaactacct gtgaaccccc attaataatg gaatcttccg
    27301 ctgtcccgtt tgccataatt tgttgtaggc cagagacgat atcggtattg atcgccttac
    27361 cataattctg aacggtttgc gtgccaccat tgatgtgtgt tttatctgtt gacccaccat
    27421 caacaatttg ttcaccactt tcgatatttg cctcagtggc taaaccatat accgtttgct
    27481 tgccaccttt gatatttgct ttatcagaag tggcactggc atatattgtt tgggtgccag
    27541 aactattaag tacagtgcca acatcttttc cataaacatc cattttgccg ttggcattaa
    27601 taatcgtatc aactgcccgg gaaccagtga cgactgttaa tgagccagcg ttttccagca
    27661 ctacattttt agcttctgaa ttcctgatgt agaaagcatc accataactg ttggttcctt
    27721 cgataagtgt tccggaagtc gtggaagcaa ttaatgcgcc gccggattgt tgctctacat
    27781 gcttagcctc tccaccgtcc tgaacctcca gaatgccgcc attgttaagt ctggtggtat
    27841 ctgttttggc ctccttctgg acgatcagtt taccgccagc atcaacggtg gtatttttcg
    27901 ccgaggtttt agccactacc gtcagttcgc cggtattttc cagcacaaca taattagcct
    27961 cccctccggt aatagtgaag tgagagagct tgttgtatcc ttcgatatca gtccctgcgc
    28021 ccgtgttggc aactaaagca ctgcccgtct cctggttaac cccatgtgca ataccgccgg
    28081 tatagacaat cagcgagcct ccggcgctaa tattgctgcc aattgccgta ccatctttct
    28141 caacaacctg ccggctcccg gaggatataa ttgttgtgtc agctttcccg ccgcttttga
    28201 tattttgcgt tacgctgttg atattggtgc ctgtggctat gccataatta ttaatattct
    28261 gtgtgccacc attaattatg gtatttgtcg cgtttcctgc aacatccata accccgccat
    28321 tatctattcg ggtcgcatca gctttagcat tggttaaaac tgacattgtt cctttatctt
    28381 taataatcgt cttgtttgcg gaatgagata cttctaaatg gcctccattt ttcagcaaca
    28441 cattgtctgc cactttattg tggatggaga atgcaccttc actattcgta ccgctcaccg
    28501 tcgtaccgtt agtgttagtt tttaaaattg caccatcgtg ctgggtaaca tttgttgccg
    28561 taccaccact aacatcaagc acgccaccag aataaacttc aataacatcc gaggtgctgg
    28621 tgttatcaac aatttgcgtg ccaccagaat agatatgcgt attttttgcc gttgacttac
    28681 tattcagaga ctgagttccg ccttcaatcg tcgtgtccag cgcacggctc tcatatactc
    28741 ttttctcacc gccattttta atggtggttg tttctactgt gctctgttca acatactgtc
    28801 gaccaccatt tatggttgtg ttcgttgcca gacttccttg cgtgatgtcc tgagagccag
    28861 acttatttat cgttgtactg tcgacatgcc cctgagttgt tactcgttgg ctgccaccat
    28921 caatgattgt tccattcgca gtccccccct ctatgcgtga agtaccgccc ttaattgtcg
    28981 tgtcattgct ggtaccacct ttatgaacat attgattgcc actttcgatt atcgtgccag
    29041 tagaaattcc tccgtactca attgactgtc tgcctccatt aatggttgta ttattagctt
    29101 gcccgttaat atcggcatgg attgatacta gttgctcgcc accagtttca ataatactac
    29161 tattagatat cccaccatag acctcctgct ggccatggtt gataatatga gtgttatttg
    29221 ttgtacctcg ttcatctact ttttggacac catctacagt ctcatcgttt accacaccaa
    29281 taacattagt agtaaaggcc gccatcacgg gcggggcata tatcaaggca gatatcaata
    29341 aggaaagtac tgagcggcga aaataatggg gactggtcct gttcataaat ttcatcctct
    29401 gaaaagtgaa tactgagtag cgtttaagcg accttagctt tgctgcaaca tcagcccaca
    29461 ggcaccagac caggggattc atcctgaaga gacagcgcaa gtgtattgtg ttcaccgctc
    29521 atcaaagaca tcatgatgaa atggtgatat tccgcataag aatgaggcat tttttaaacg
    29581 cagtgcgctg aagtgtggtt ggataaaaaa gtcaatctat tcaggaaaca cgggcgtatt
    29641 cttttctttc gacaatcagg cagttggcaa aataaaatga tttacataat cgtttctgat
    29701 gaatatcttc tgctcacata aaaatcacac aataactttg agatcgcaga ttgttttact
    29761 tttacagcat tcgtcccccc attgttgggc aaatatagat tgggccagag cacgaaagtt
    29821 aataccacgt tcgcacagct cctccaacag cacgacaaga tgccacatac tgcgccccag
    29881 tcggttcagt ttacagacta gcagtgcgtt ccctgccgat gatgtcctga ccagtttttt
    29941 cagtccggac ctcctgccac ggttttacgt tgaagtggta accctgagca cgcagttctt
    30001 cagtcaggcg tggtgcaccg taacgctgtt attgctgggt aaaatcaaaa aactttcagg
    30061 cagctaagga aagttgaacc agacattaga tgaaatattt caaccaatta cagcaccaat
    30121 tcagtcactg ccagcccacc aaataaatca aggggttaca tgaaaacgta gccccttttt
    30181 ctttggtagt gacactaaaa tggatgtagt gtgaagaata atcccgttta ctcaatcaat
    30241 aatacatatt gtttcaatct acgttattat ctctttgtaa aaattgccat ttattaatca
    30301 ttgaaaactg cttttagaac ttgatacaac gggactagtc acaacaggac tattctcaac
    30361 gggatcatcc tcagaggaac tatcatcaaa gtcatcatcc ataaataaaa tatcatcgaa
    30421 tggtgccacg cccgtgatga gttttatttt attattacga tcagtcaata ctccacttaa
    30481 accgttttcg ctcacaggtt ttaatgattt ttcattactc ttgttgtaag caggcgcatt
    30541 aaaaatacac ggagtatcaa catcaaacaa tgacgttccc cagttcacat attgaatatc
    30601 atagttactg aaaatccaat ccacgtaaat tatataaacc accctcttct ttttggagag
    30661 taatgttaat tttggctatc tcccggacat catcgccatt tttgtatttg aataccgttt
    30721 caagatgttc tccagacagc ttgacttcag gaaataattt gaaatcaaag cctatattat
    30781 tatgtgtcaa cgtagatgaa caaaaaatgg aaaaagcttg cagtgctgaa ttatagctat
    30841 cgattttatc ttgaggctga gctcttggta aaaacttata gcaacactca taaaaa
//
LOCUS       DAZFZC010000059        30355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFZC010000059 DAZFZC010000000
VERSION     DAZFZC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.059"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..732
                     /locus_tag="ACHKXP_002968"
                     /pseudo
     CDS             1..732
                     /locus_tag="ACHKXP_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
     gene            760..1718
                     /locus_tag="ACHKXP_002969"
                     /pseudo
     CDS             760..1718
                     /locus_tag="ACHKXP_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1730..2191
                     /gene="yibG"
                     /locus_tag="ACHKXP_002970"
     CDS             1730..2191
                     /gene="yibG"
                     /locus_tag="ACHKXP_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO7647934.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2815..3336
                     /locus_tag="ACHKXP_002971"
                     /pseudo
     CDS             2815..3336
                     /locus_tag="ACHKXP_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
     gene            complement(3796..4932)
                     /gene="yibH"
                     /locus_tag="ACHKXP_002972"
     CDS             complement(3796..4932)
                     /gene="yibH"
                     /locus_tag="ACHKXP_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7647935.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(4935..5297)
                     /gene="yibI"
                     /locus_tag="ACHKXP_002973"
     CDS             complement(4935..5297)
                     /gene="yibI"
                     /locus_tag="ACHKXP_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7647936.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5834..7747
                     /gene="mtlA"
                     /locus_tag="ACHKXP_002974"
     CDS             5834..7747
                     /gene="mtlA"
                     /locus_tag="ACHKXP_002974"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO7647937.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7977..9125
                     /gene="mtlD"
                     /locus_tag="ACHKXP_002975"
     CDS             7977..9125
                     /gene="mtlD"
                     /locus_tag="ACHKXP_002975"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7647938.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9125..9712
                     /gene="mtlR"
                     /locus_tag="ACHKXP_002976"
     CDS             9125..9712
                     /gene="mtlR"
                     /locus_tag="ACHKXP_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO7647939.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9724..9933)
                     /gene="yibT"
                     /locus_tag="ACHKXP_002977"
     CDS             complement(9724..9933)
                     /gene="yibT"
                     /locus_tag="ACHKXP_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO7647940.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10218..10580
                     /gene="yibL"
                     /locus_tag="ACHKXP_002978"
     CDS             10218..10580
                     /gene="yibL"
                     /locus_tag="ACHKXP_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO7647941.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            10954..12609
                     /gene="lldP"
                     /locus_tag="ACHKXP_002979"
     CDS             10954..12609
                     /gene="lldP"
                     /locus_tag="ACHKXP_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO7647942.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            12609..13385
                     /gene="lldR"
                     /locus_tag="ACHKXP_002980"
     CDS             12609..13385
                     /gene="lldR"
                     /locus_tag="ACHKXP_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO7647943.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            13382..14572
                     /gene="lldD"
                     /locus_tag="ACHKXP_002981"
     CDS             13382..14572
                     /gene="lldD"
                     /locus_tag="ACHKXP_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO7647944.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            14770..15243
                     /gene="trmL"
                     /locus_tag="ACHKXP_002982"
     CDS             14770..15243
                     /gene="trmL"
                     /locus_tag="ACHKXP_002982"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO7647945.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(15296..16117)
                     /gene="cysE"
                     /locus_tag="ACHKXP_002983"
     CDS             complement(15296..16117)
                     /gene="cysE"
                     /locus_tag="ACHKXP_002983"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO7647946.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(16197..17216)
                     /gene="gpsA"
                     /locus_tag="ACHKXP_002984"
     CDS             complement(16197..17216)
                     /gene="gpsA"
                     /locus_tag="ACHKXP_002984"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO7647947.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(17216..17683)
                     /gene="secB"
                     /locus_tag="ACHKXP_002985"
     CDS             complement(17216..17683)
                     /gene="secB"
                     /locus_tag="ACHKXP_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO7647948.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(17746..17997)
                     /gene="grxC"
                     /locus_tag="ACHKXP_002986"
     CDS             complement(17746..17997)
                     /gene="grxC"
                     /locus_tag="ACHKXP_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO7647949.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(18139..18570)
                     /gene="yibN"
                     /locus_tag="ACHKXP_002987"
     CDS             complement(18139..18570)
                     /gene="yibN"
                     /locus_tag="ACHKXP_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO7647950.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            18815..20359
                     /gene="gpmM"
                     /locus_tag="ACHKXP_002988"
                     /gene_synonym="pgmI"
     CDS             18815..20359
                     /gene="gpmM"
                     /locus_tag="ACHKXP_002988"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO7647951.1"
                     /translation="MLVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            20393..21652
                     /gene="envC"
                     /locus_tag="ACHKXP_002989"
     CDS             20393..21652
                     /gene="envC"
                     /locus_tag="ACHKXP_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO7647952.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            21656..22615
                     /gene="yibQ"
                     /locus_tag="ACHKXP_002990"
     CDS             21656..22615
                     /gene="yibQ"
                     /locus_tag="ACHKXP_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO7647953.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPATVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(22602..23636)
                     /gene="waaH"
                     /locus_tag="ACHKXP_002991"
     CDS             complement(22602..23636)
                     /gene="waaH"
                     /locus_tag="ACHKXP_002991"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO7647954.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLH"
     gene            complement(23875..24900)
                     /gene="tdh"
                     /locus_tag="ACHKXP_002992"
     CDS             complement(23875..24900)
                     /gene="tdh"
                     /locus_tag="ACHKXP_002992"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO7647955.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLAAIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(24910..26106)
                     /gene="kbl"
                     /locus_tag="ACHKXP_002993"
     CDS             complement(24910..26106)
                     /gene="kbl"
                     /locus_tag="ACHKXP_002993"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO7647956.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26320..27252
                     /gene="rfaD"
                     /locus_tag="ACHKXP_002994"
     CDS             26320..27252
                     /gene="rfaD"
                     /locus_tag="ACHKXP_002994"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO7647957.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIIAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            27262..28308
                     /gene="rfaF"
                     /locus_tag="ACHKXP_002995"
     CDS             27262..28308
                     /gene="rfaF"
                     /locus_tag="ACHKXP_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO7647958.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            28312..29271
                     /gene="rfaC"
                     /locus_tag="ACHKXP_002996"
     CDS             28312..29271
                     /gene="rfaC"
                     /locus_tag="ACHKXP_002996"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO7647959.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPLMNLNNLEAAAVYKKITLI"
     gene            complement(29319..>30355)
                     /locus_tag="ACHKXP_002997"
     CDS             complement(29319..>30355)
                     /locus_tag="ACHKXP_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO7647960.1"
                     /translation="MNLIWYEFYKTEDVVYRNAYRGYLESGKLLVFSSLTFLLLSKNK
                     FRQNEKLHFISAILVQIIMLCRAYYQGIYLHADRIPLSAMNGNIGQMGAATIAAYVIT
                     FTTLYSSIVFIKMQSKYKWFLFYANFALSFAAVTMTGTRAAIFTFPLMIMVILLIQYR
                     NQRVFLFKGLSGFFFLFLVCGLIFNKGIERRINALQTDVISYTTKNNSNSSVGARFAM
                     VNAGIKGSPDTLGWQSLEERAKKIKALSLDNNIYNSVLPFLDVHMHNEIVESLSTKGK
                     IGLLVLIMFYVAMIYYCIREKKYILLVFPASIMLFGISDVITHAKPIPASWIVCLFLS
                     TSFLSKKEPQ"
BASE COUNT         7837 a         7541 c         7621 g         7356 t
ORIGIN      
        1 ttgaaaagga tttcatctgc cagagtatta cagttaagag gggggcaaga cgctgttaga
       61 ttggcaattg aattctgcac tgacaaaaat tatatccgta gagatatcgg agcatttata
      121 ctcgggcaaa tataaatttg caaaaaatgc gaagataatg tttttaatat tttgaacaat
      181 atggcattga atgataagag cgcttgcgtt cgagctacgg caatcgagtc aacggctcag
      241 cgatgcaaga aaaacccaat ttattcacct aaaatagtag aacaatctca aattactgct
      301 tttgataaat cgactaatgt cagacgtgct acagcatttg ctatttctgt tatcaatgat
      361 aaagcaacaa ttccactatt gattaatctg ttaaaagatc caaatggaga tgtcagaaac
      421 tgggccgcat ttgcaataaa tatcaataaa tatgataata gtgatattag ggattgtttt
      481 atggagatgc ttcaggataa aaatgaggaa gtccgtattg aagcaataat cggactttcc
      541 tacagaaaag ataaaagggt tttatctgtt ttatgcgatg agttaaaaaa aaatactgtt
      601 tatgatgata tcattgaagc tgcgggtgaa ttaggtgata aaacgctact tcctgtttta
      661 gatactatgt tgtacaagtt tgatgacaat gaaattataa cttccgctat tgataagctg
      721 aagcgttcat agcgtgagtt gcctatgcac agtgggggat tcccgccggc acggtgcaga
      781 tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct
      841 ggctggcatc gtgcggcctg accgtggagc agatgaaaaa ccagatgggc ccggtgtaca
      901 cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg ctggcgctca
      961 tcagcacgga aggggcaaca gcgtggtgcg cagaatatga tgaatggggc aacctgctga
     1021 gtgatgagaa cccgcatcat ctgcagcagc tcattcgcct gccgggtcag cagtatgatg
     1081 aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag gggcggtata
     1141 tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat ccgttgaatc
     1201 cggtcataaa tgtagatccg caaggtttgg ttgatataaa tttatacccc gaaagtgatc
     1261 ttatccattc tgtagctgat gagattaata tcccaggcgt tttcacaatc ggggggcatg
     1321 gtacccccac atctattgaa tccgcaacgc gcagtatcat gacagctaaa gatctagcat
     1381 atctaattaa atttgatggg aattataaag atgggatgac agtttggtta ttttcttgta
     1441 atacaggtaa aggacaaaat tcatttgcta gccaattagc taaagagtta catacaaatg
     1501 taataggacc tgacacgcta tggacgtggt gggggcgagg aactaatggt aagttaaaaa
     1561 tggatacagt gctaacagca ccaacgaacc ttaattcaaa taaggatcta atggctataa
     1621 caacaaaaga ccttggtaat tggataacat atgggccatc tgggcacccc atttctaata
     1681 tgcaaggtac gccagaaaaa cccagtgata taagataggt tgtagatgta tgaaagcatg
     1741 cttgttacta tttttttatt tttctcttat tggtcaattg catggtgctg atgtgaaaat
     1801 aaaagaaaac gaaagtgtga tgggatctac agcaatgacc tatgatttaa gtgaagaaaa
     1861 gctgatgaaa ctgaagtata aatcacaaca tggtgattca gaggcatcat ttcgactata
     1921 tcaatattac tgctttacta aaaataatat tgataaacaa ttgcgattct tggaaagatc
     1981 agcatctcag gggaatgtta cagcgcagtt taattatggg gtctttttat cagatacaaa
     2041 tccaacatta tcagaatatt ataatttgaa tagagctatt tattggatgg aattcgctgt
     2101 aaataacggt aatattgatg caaagagcaa acttcaagag ctcaaaaagc taaagcgaat
     2161 ggatagaagg aagaataagg agaatccgta aaagcaatag ttcatcagac tgccgcagca
     2221 gtagtatgat gatgaatcag ggctgtacta taactagtat cggtaacatt aaccgcagca
     2281 ggggcggtat aacactcagg ctccgattgg gctggaaggt aggtggaatt tttatcagta
     2341 tccgttgaac ccgatttctg gaatcgatcc ccttgggatt agccacctgt ttatacagca
     2401 taactttagg tatgctttcg tgtgtttcag atataccatg tgatgataat ttttatgatg
     2461 tgcttagtat tccagtcgct tctggtaata atatgcaatg taaaaataat ccccgttgta
     2521 cacatttata aatagatggc caatacctca aggcctatgg agttggaatg ttaatgaccc
     2581 gggagcaagc aatagaaaac ctgacggcat acgattagta ccgtcagtta atacaggaac
     2641 ttataatcgc aatggatttt caatacatag ttgtttaaac gcttttggtc cgtctttagg
     2701 acctcgattc tgttccgagg gttgtataac tggtttatct aatgacatgc aaaaattaaa
     2761 tgagttaata ttttcagagc cagatagcac attaacagtt acggactaaa aaatatggca
     2821 aggttattaa caattgtatt tttttctcta tttctgcgag tgcagttact gcaagacaat
     2881 ttcagagaga gttagatttg gcagacaaag caatattatg ggcactaata tcagccagta
     2941 ctaaagaggg acgaaaagcg tgttcactaa gctattttgc ctgtaaggcc gcagaggctg
     3001 aactcggact ggcatatatg gctgcgaatg ataacaagga atttctcaca tccttatcta
     3061 atataatgag gtacaaaata gatgctggac tttccgaatc ctatacatgc tatttattaa
     3121 gtaagggaaa gatcataaga ccatatctga aaaatctaaa tccactccaa cttgccgctg
     3181 attgcattga aacagtaaat aaaataaaag ataagaataa aaaaaatcat tgatataaat
     3241 tcagttaata tttgtagtga tgataaaaat ataaaattga gagttaatag tactattatg
     3301 gccattgatg attctataaa gtgcatcgat gaatgataac ttaattttat ttaaaaatat
     3361 attaataact cactctccag gagttttttt aacttagttt tataatgaat acgataatta
     3421 aaagtgaaga agaaaatagt tgtatacata tattccatga ccctacattg tgatctatag
     3481 attaaatatt ttttaaatgt gactgtcatc cttcagataa taaagccatc atcgcaatag
     3541 atggaaaagc actcctgctc ttttttgatt agagccgtcg caggaggaac aactcatatc
     3601 atcattgcga tctcgacgat gtatagtctc atcctgcggc agaacaagac agataaaaaa
     3661 tccaatgaga gcacactcat ccagaactta ttaacataat gaatattaaa gaaaaatcat
     3721 cgcagctaat gaagtaatat gcaaaaataa ttagtcctta caaggagcaa agttccataa
     3781 tctcccccct ccccctcaat gatccaaata aagataatgc atccagctgg tcattcttag
     3841 cagcactttc cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg ccacctgggc
     3901 gtagatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa tggttcccag
     3961 cacaccgtcc gtgccgggca cgaccgttaa tgattgcaat accccctgcg cctgataaga
     4021 accgcctggc acgacaggta aaatactagt cagtttgccg tgaaacacct gcccaggtag
     4081 cgcgttaaac accacttccg catcatcgcc gggtttcaga cgtaacagcg agttttgccg
     4141 aaattgggcg acaatttgcc gtttttgctc ggggatgaag accatcaccg gacgcagcgg
     4201 caaggcagct gcgtatgtac ctgggcggat cagtacctga gtaacgtagc cattgctcgg
     4261 cgcgcgaatg acagtctgct caaggttata ttttgcttca gtaagttgcg ctcttaagct
     4321 cacaatctgc gattgctcgc cgttaaccat actgtcgagc tggctctgga tctgcgcctg
     4381 ctccgccacc gagcctttca ccagcgcatc ctgcgcgagg aaattttgcc gcgcatcgtc
     4441 gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaagt aacgttgata
     4501 atttttaaac agacggtcgc gctccgctga aacctgggtg gtgttggcct gcgcttcagt
     4561 gagctgcgca cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag cctgaagtct
     4621 gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat
     4681 aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg gcgtgatagg
     4741 gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt tcatcaacaa
     4801 aatcaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg tccactgatt
     4861 taccggaatg cggaagattt taaagaccgc ccacgccagc gccacgtaag ttaaaacaat
     4921 caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt ctcggcggcg
     4981 agttgatgct ccagccggga aattcggtca gaaagtgagg cgatttccgg gtcagtcgct
     5041 ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg atagagcgtc
     5101 gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac ccagcccgcc
     5161 gtatgaatag cgtcggcatg gggatggttg cgctttttgg caatcagata agggatgtca
     5221 tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac tcccaacgcg
     5281 aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt tttgggctgg
     5341 cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga ttctcgccat
     5401 aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg attgtatgac
     5461 gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata tcttgtgatt
     5521 cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacactactc acatttaaat
     5581 gccattttta gcgaaaatcg ccgccttgtt gcttttttac acaagcgttt tgtgatgaac
     5641 gtcacgtcaa ttacctctct accccctata tttatgtgat tgatatcaca caaaaggccg
     5701 tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag gtccggctac
     5761 ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag caaacataag
     5821 aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg gtcgtttcct
     5881 cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca tcaccgcgtt
     5941 atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg ggccgatgat
     6001 cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag gcggcgaacg
     6061 tggcggcgta gtcggtgcca tcaccaccat gggcgttatc gtcggcgcag acatgccgat
     6121 gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc acttcgaccg
     6181 ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact tctccgcagg
     6241 catcatcggg atgatcctcg ctattctggc attcctcggc attggcccga ttgttgaagc
     6301 cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca tgctgccgct
     6361 ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca ttaaccacgg
     6421 tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa tcttcttcct
     6481 gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt tctttggtcg
     6541 tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg ggggtatcca
     6601 cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag tcatcctcgg
     6661 cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt ctccggcatc
     6721 tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttact tcgctaacat
     6781 cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctgctattt tgctgaaaac
     6841 cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc aggacatgaa
     6901 agctgagtct aaaggcgcat ctccgctgtc tgctggcgat gtgactaacg acctgagcca
     6961 cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tccagtgcga tgggcgcagg
     7021 cgttctgcgt aagaaaattc aggatgcagg tctgtcgcag atttctgtta ctaacagcgc
     7081 gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc tgaccgaacg
     7141 cgctatgcgc caggttccgc aggcacagca tatttcgctg accaacttcc tcgacagcgg
     7201 cctgtacacc agcctgaccg aacgtctggt tgctgcccaa cgccacacgg caaacgaaga
     7261 gaaagtaaaa gacagcctga aagacagctt tgacgattcc agtgctaacc tgttcaagct
     7321 aggcgcggag aacatcttcc tcggtcgcaa agcggcaacc aaagaagaag cgattcgttt
     7381 tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc aggcgatgct
     7441 ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc cacacggtac
     7501 ggttgaagcg aaagatcgcg tactgaaaac gggcgtcgtg ttctgccagt acccggaagg
     7561 cgtgcgcttc ggtgaagaag aagatgacat tgcccgtctg gtgattggta ttgctgcccg
     7621 taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg atgagtccgt
     7681 catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgc tggcaggtcg
     7741 taagtaatcc aatcccaccc tctccacatg gagaaggtgg ggttaattgc ctgatgcgct
     7801 acgcttatca ggcctacagg atgcatcaca atttgttgaa tttgcacgtt cttgtaggcc
     7861 ggataaggcg cttacgccgc atccggcgct gcccctctcc tcacggagag ggtttgggtg
     7921 agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga
     7981 aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag
     8041 acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg
     8101 cccgtcatag ctatcaggta catgtggttg gtgaaaccga gcaggtagat accgtttccg
     8161 gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctgattgct caggttgatt
     8221 tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggca atcgccaaag
     8281 ggctggtgaa acgtaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa
     8341 acatggtacg cggtaccacg cagctgaaag gccatgtgat gaacgccctg ccggaagacg
     8401 ccaaagcgtg ggtagaagaa cacgttggct ttgtcgattc cgccgttgac cgcatcgtac
     8461 cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaacc ttcagcgaat
     8521 ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa
     8581 ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta
     8641 taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgcg attctcgacg
     8701 agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcagta ttgatcaagc
     8761 gctacggctt tgacgctgac aagcatgcgg cgtacatcca gaaaattctc ggccgttttg
     8821 agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccactg cgtaaactga
     8881 gtgctggcga ccgtctgatc aagccactgc tcggtacgct ggaatatggt ctgccacata
     8941 aaaacctgat tgaaggtatt gccgctgcaa tgcacttccg cagtgaagat gatccgcagg
     9001 ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt
     9061 ccggtcttga tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc
     9121 aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca
     9181 ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg
     9241 caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctc ctgaccgagg cggtaaatct
     9301 tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt
     9361 actcgacggc gatggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt
     9421 gggcgtcatt aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga
     9481 gctaaatcac gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg
     9541 tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag
     9601 tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc
     9661 actgactgag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc
     9721 tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg
     9781 tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag
     9841 ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat
     9901 aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc
     9961 gggaatgcgc ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac
    10021 gtgctgaatc ctcaataatc atccgctgct gtttattatc ttttttctat agatttctta
    10081 attaatcaac gaattgcgta gtttttgagc atgttaatga ttacgttaca gaactattac
    10141 aatgtcatta gttgtgaaca aagcacctgg tcgcgcatac taggggctat aaatttatct
    10201 ttatcagaag ccatcacatg aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc
    10261 gcctggacgc catccgccac cagcaggccg atctttcgct ggttgaagcc gcagacaaat
    10321 atgccgagct ggaaaaagag aaagccacgc tggaagcaga aattgctcgc ctgcgtgagg
    10381 ttcatagcca aaaactgagt aaagaagcac aaaaactgat gaagatgccg tttcagcgcg
    10441 caattaccaa aaaagagcag gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg
    10501 tcgttgtgca cccaatgacc gcactgggcc gcgaaatggg cctgcaggag atgaccgggt
    10561 tttcaaagac cgcgttttaa gaacacagta tctacagggt gattctgcac attcctatag
    10621 gccgagtaag gtgttcacgc cgcatccggc aagataaggc gctctggatc aacaatctaa
    10681 gggcaattct ctgatgagga ttgccctttt ctttaccaga catctccctt caccaaaaaa
    10741 ttggccctac caattcttcg cttatctgac ctctggttca caattcccca attaaaactc
    10801 acatcaacgt tgccaataca taacatttag ttaactattc attgtcatta tccctacaca
    10861 acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca acagactcat
    10921 tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca aaactacgat
    10981 cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct gtttttcttc
    11041 tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtggcaatc
    11101 gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg
    11161 gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc agcagtgttc
    11221 gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc tattctttcg
    11281 ataaccccag accagcgcct gcaaatgctg atcgtcggtt tctgtttcgg cgcgttcctc
    11341 gaaggagctg caggctttgg cgcaccggta gcaattaccg ccgcattgct ggtcggtctg
    11401 ggctttaaac cgctgtacgc cgccgggctg tgcctgattg ttaacaccgc gccagtggca
    11461 tttggtgcga tgggcattcc aatcctggtt gccggacagg taacaggcat cgacagcttt
    11521 gagattggcc agatggtggg gcgtcagcta ccgtttatga ccatcatcgt gctgttctgg
    11581 atcatggcga ttatggacgg ctggcgcggt atcaaagaaa cctggcctgc ggtcgtggtt
    11641 gcgggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg gccggagctg
    11701 ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct caaacgctgg
    11761 cagccagtgc gcgtattccg ttttggtgat ttgggggcgt cacaggttga tatgacgctg
    11821 gcccacaccg gttacactgc gggtcaggtg ttacgtgcct ggacaccatt cctgttcctg
    11881 acagctaccg taacactgtg gagtatcccg ccgtttaaag ccctgttcgc atcgggtggc
    11941 gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact ggttgcccgt
    12001 atgccgccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt tgactggttc
    12061 tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg gctgaagatg
    12121 aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc tctgcccatc
    12181 tactccatcg gtatggtgct ggcattcgcc tttatttcga actattccgg actgtcatca
    12241 accctggcgc tggcactggc gcacaccggt catgcattca ccttcttctc gccgttcctc
    12301 ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct gttcgccgcg
    12361 ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt tgccgccaat
    12421 accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat cgcctgtgcg
    12481 gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg
    12541 atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt aacgtggatg
    12601 attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg
    12661 cgctgattga tgaaaaaaac ctggaggcgg gcatgaagtt gcccgctgaa cgtcaactgg
    12721 cgatgcaact cggcgtatcg cgtaattcac tgcgcgaggc actggcaaaa ctggtgagcg
    12781 aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt catgacacat
    12841 ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat ccggattaca
    12901 gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg catgcggcaa
    12961 tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca acgctaagtg
    13021 aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct
    13081 cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctcag
    13141 tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa ctgaccgaac
    13201 aacatcaggc tgtcattgac gccatttttg ccggtgatgc tgacggggcg cgtaaagcaa
    13261 tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa gatcaggctc
    13321 gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg gagaaaaacg
    13381 catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc tgccgccgtt
    13441 cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga
    13501 agatttgtca gaagtggcgc tgcgccagcg tattctgaaa aacatgtccg acttaagcct
    13561 ggaaacgacg ctgtttaatg agaaattgtc gatgccggtg gcactggctc cggtgggttt
    13621 gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg
    13681 tattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg cgccagccat
    13741 caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta tgcgtaacgc
    13801 gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg atatgccgac
    13861 accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccgaacg cggcaatgcg
    13921 ccgctacttg caagcggtga cacatccgca atgggcgtgg gatgtgggcc tgaacggtcg
    13981 tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac tggaagatta
    14041 catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc ttgaatggat
    14101 ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg aagatgcgcg
    14161 cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg gccgccagct
    14221 ggacggtgta ctctcttccg cccgtgcact gcctgctatt gcagatgcgg tgaaaggtga
    14281 tatagccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc gtatgattgc
    14341 gctcggtgcc gacaccgtac tgctgggtcg tgctttcttg tatgcgctgg caacagcggg
    14401 ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac
    14461 gctgactggc gcgaaatcga tcagcgaaat tacgcaagat tcgctggtgc aggggctggg
    14521 taaagagttg cctgcggcac tggctcccat ggcgaaaggg aatgcggcat agtcgtttgc
    14581 gccccctcac cctaaccctc tccctcaggg agaggggacc gttcggcgct gtatgtactc
    14641 cctcactctg aaacgacacc gcactctttt tttctccctc gcccctccgg ggagagggcc
    14701 ggggtgaggg gaaaaggccg cactgctcac caattttctg ctatcctgcc cccgcactaa
    14761 gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa tactggcaac
    14821 atcatccgtc tttgcgctaa taccggcttt cgtctgcata tcatcgaacc gatgggattt
    14881 gcctgggacg ataagcgcct gcgccgcgcg gggctggact atcacgagtt taccgccgtt
    14941 acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg cctgttcgcc
    15001 ctcaccacga aaggtacacc tgctcacagc gccgtaagct atcaggatgg cgattatctg
    15061 atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct gcccgctgaa
    15121 caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc caatgcggtg
    15181 tcggtagtgg tgtatgaagc ctggcggcag ttggggtatc cgggagcggt attgagagat
    15241 tagttactgt aggccggata gatgattaca tcgcatccgg cacgatcaca ggacattaga
    15301 tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc atatccattg
    15361 atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg gcggtggtat
    15421 gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg cgcccaactt
    15481 caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga attttcgggt
    15541 gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga atcgatacgt
    15601 cgttttcaat caccgccgtt tcaccaacga cgatgcctgt cgcgtggtca agcatgatac
    15661 cgcgaccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa acctggtttt
    15721 gcagaaagat tgccagtgcg cgacgcccct gattccacaa ccagtgaccg atgcgatagg
    15781 cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta tcgactgccg
    15841 ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt tccgggtcag
    15901 cggcgtaggc ttcttccacc acttcacgga tagcaatagc aggcataatt ggcgatgaca
    15961 gcttgttcgc cagcatgtag ctcagtgcac tgccaaggtt ttcgtgcttg agtagcgtcg
    16021 cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct tcggctttaa
    16081 tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca cgatgagata
    16141 atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc ctggggttag
    16201 tggctgctgc gctcgtcctt gcgtgcacga cctagtaaag tcaatgctgc ctcgcgcgcg
    16261 ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc aacgccgaag
    16321 cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac ctgaccaatc
    16381 ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcatgccaaa acggcggtta
    16441 cgcgactggt tgtcggtaca ggtaagcacc agatcgccaa gccccgccat gcccataaag
    16501 gtggcagggt cggcacccag cgccgcacca agacgcgaca tttcagccag cccacgggtg
    16561 atcagcgccg tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc cgcaccaatg
    16621 gcaataacgt ttttcaccgc gccgccaagc tgcacgccaa tgaaatccgg attgctgtaa
    16681 acgcggaaac ttttgccgca gtgcagcagc tgctggagat catcggcaaa ggtctgatcg
    16741 gtcgaggcca gcgaaatagc tgtcggtaaa cctgccgcca gttctttcgc aaacgttggg
    16801 ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac gtcctgtaac
    16861 agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc atcaggacgc
    16921 atcagtggtt taatctggcg cagcacttca ccaaagacat ggctgggtac gacgacgaga
    16981 atattacggc tggctgccag cgcagtggcg agatcgcttt caagatggag cgtatcggga
    17041 aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt tgcgatatgt
    17101 tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt gatggcaaga
    17161 gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg gttcatcagg
    17221 catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc atgaacagcg
    17281 catcgaagtt aaccggcgca aggttcagtt gcgggaatgt accgcgggat accatgctgg
    17341 tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgctccc aggcaatgcg
    17401 ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga acttcacaca
    17461 ggaacgcggt ttcttcgccc aaagaggccg ttacggtaac acgcagtacc acttcgtata
    17521 cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct ggttgccaat
    17581 ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg gtataaatac
    17641 gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga aaacccttaa
    17701 gtgttgtcct taaatactgc gcaatgtaat gccaacgttc acacgttatt tcagcagggg
    17761 atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa tgtgctgtgc
    17821 gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt ttgatcatct cttcacgctt
    17881 ggcggcgttg ccatcgatcg gcagctcctg gaaactcacg cccttgctgc tcagcagtgc
    17941 ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat tggccataaa
    18001 ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggcagcaa aatggagtgt
    18061 ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg gcctgcatca
    18121 tgaaagacga caggtaaatt atttgccgcg caccaaaggc aagttttcgc cagcccagcc
    18181 agcgacgcct tctttcagca cgaatacttg cgcaaaacca gctttcgtca gtgcgtttgc
    18241 aggctcctgg cactgcatgc cagaaccgtc taccacgata accggtttgt ctttgtgctt
    18301 ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattaa tagaacctgc
    18361 gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag cgtcttcttt
    18421 gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg tcaggctctt
    18481 aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca gtatgggatg
    18541 acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac gtagtgatta
    18601 aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc gcgttaactg
    18661 gaatgcaatt ttgcggggcg cgacgaaaaa aaagcgtaaa tctggcatcg gcttgccgcg
    18721 agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta gtaaaattac
    18781 gcaaattttg actcttgagt atgaggttgt cgcaatgttg gtttctaaaa aacctatggt
    18841 actggtgatt ctggatggct atggctatcg cgaagaacag caggataacg ccatttttag
    18901 tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc taatcgacgc
    18961 ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag taggccacgt
    19021 taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg ttgaaatcaa
    19081 agatcgcgct ttctttgcta atccggtgct gactggcgcg gtagataaag cgaaaaacgc
    19141 aggcaaagcg gtacacatta tgggtctgct ctccgcaggc ggcgtacaca gccacgaaga
    19201 tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa tctacctgca
    19261 cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc tgaaaaaatt
    19321 tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg gtcgttacta
    19381 cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc tgctgactct
    19441 ggcgcagggt gagttccagg ccgatactgc cgttgctggt ttgcaggcag cttatgctcg
    19501 cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc agccagatgc
    19561 ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc gcgcgcgcga
    19621 aatcactcgt gcattcgtaa acgcagattt cgacggcttc gcgcgtaaga aagtggttaa
    19681 cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg ttgcttaccc
    19741 acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca aaactcagtt
    19801 gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg gtggcgtaga
    19861 agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg ctacctacga
    19921 tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg caatcaagag
    19981 cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag gtcacaccgg
    20041 ggtgatggaa gcggcggtta aagcagttga agcgctggat cactgcgtgg aagaagtcgc
    20101 gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg gtaacgctga
    20161 gcagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc tgccagttcc
    20221 gctgatttac gttggtgata agaacgtgaa agcggttgaa ggcggcaaac tttctgacat
    20281 cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga ctggtaagcc
    20341 gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata ccatgacacg
    20401 ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg ttcttagcgc
    20461 tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac tcaaatctat
    20521 tcaggctgac atcgccgcaa aagagcgcgc ggtacgccaa aagcaacaac aacgcgcaag
    20581 cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc gtaagctgcg
    20641 cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga acgcgtcgat
    20701 tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac aactggatgc
    20761 cgcattccgt cagggcgagc ataccggtat tcagctgatt ctcagcggtg aagaaagcca
    20821 gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac aagaaaccat
    20881 tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac tggaagagaa
    20941 acagagcgag caacaaacgc tgttatatga gcagcgcgcc caacaggcga aactgactca
    21001 ggcgctgaac gagcgtaaaa agacgctggc agggctggag tcttccatcc agcaaggtca
    21061 gcaacagttg agcgagctgc gcgccaacga atcccgtctg cgtaacagca ttgcccgtgc
    21121 ggaagccgcg gcgaaagcgc gtgcagaacg agaagcacgt gaggcccagg cggttcgcga
    21181 ccgccagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa gcgaaaaatc
    21241 gctgatgtcc cgtaccggtg gtctgggcgc gccgcgtggt caggcattct ggccggttcg
    21301 cgggccgacg ctgcatcgct atggcgaaca gctacagggt gaattacgct ggaaaggtat
    21361 ggttatcggt gcttctgaag gtactgaagt taaagcgatt gccgacggtc gggtgattct
    21421 ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcatggta aaggcgacat
    21481 gagtctttac ggctataatc agagcgcact ggtgagcgtt ggttcgcagg ttcgcgcggg
    21541 ccagccgatt gcactggtgg gcagcagtgg cggtcagggt cggccttcac tctatttcga
    21601 aattcgccgc cagggtcagg cggtcaatcc acagccgtgg ttgggaagat aagttttgtt
    21661 tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct cccccgtact
    21721 tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgcaca acgaaaacca
    21781 ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac cgcacgccag
    21841 agagatggcg accaaagcgc ataacagcgg gcacgaagtg ttgattcatc tcccgatggc
    21901 accgttgagt aaacagccgc tggagaaaaa tacgctacgc ccggagatga gcagcgacga
    21961 aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg ggatcaacaa
    22021 ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga tgcaggcgct
    22081 ggagcgttac aatctttact tcctcgacag cgtaaccatc ggtaataccc aggcgatgcg
    22141 cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtattcctcg acgattcgca
    22201 aaatgaagcg gacatccgtg tgcaatttaa tcgcgcaatt gacctggcgc gtcgcaacgg
    22261 ttcgaccatt gccattgggc atcctcaccc tgcaacggta cgcgtgttgc aacagatggt
    22321 ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga atgaaccgca
    22381 ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat gcaccgcgta atccgttccg
    22441 tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta atcgcttctt
    22501 tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc agcatcagtg
    22561 gcaaggctgg ggcaagcagc ccgaagcggc aaaacttaac gctagtgcaa attaaaggcg
    22621 ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa gcggaaagac
    22681 cacagtaacg cctggtaacc caccttcacg ctgcgcacat tggtaatcag gcgcttatac
    22741 ataccggaag taaatatctc ggcaatcatc cgttggcggg taagaatatc cggctctttg
    22801 cgcaccgcat ggcaaacacg caatgcttcg taagttattt gctgatgaaa ttccggataa
    22861 atcgtaattt tgtcggcata atttcgattt aatttctcca gcaggcgggt aatcttaata
    22921 tagtgacgtt gataattaag gtttttattc ccttgtctat gcaaccgact cactgacgta
    22981 ttatgcagat aatatttata taacgattgc tcggtatatc gcgcacgcaa agcgttaaac
    23041 atgaattctg ttgtccagac aatatcctga tgatgtaatc cggcaataaa tttaatgtta
    23101 tttttaacaa taacatcgcg gcgataaacc cccatccaga caacgtgagt ccaacggcgc
    23161 gaagaaagcc ccatccgcag ccagtccggg ccggttaata cgccggttga gcgaaggcga
    23221 tcgctgggga tggattgcca ggtttctccc gtttcacgaa aacaccagtc agcgttgcac
    23281 tgcgctacat cgaggtcgtc ctctaacgcc atggtcatca gcgtttcgta catggtggga
    23341 tagacttcat cgtcagcatc gacaaaagcg acatatttgc ccgttgccac ctcgatccca
    23401 cgattacgcg ccaccgatgc gccagcattc gcctgatgca acaaacgaac gtgcggatag
    23461 ttttctgcgt aatgctttgc tatttcaaca gaattatccg ttgaaccatc gttaataata
    23521 atgatttcca gagcagtcca ggtttgcgta attaaagatt ccatacaagt gcggaaatca
    23581 tcgcccgcat tatataacgg aataataaca ctaagtttat tggtgctgtt catcataaat
    23641 taccgacgac ctctgaagtt atcctgacgt tttacaggct ttgtattaag aaactattaa
    23701 gcctgtgaga tattattttt acaggtgaga gagtcacttc agggtagaga gataatgaga
    23761 gcagctatat aagagatcag cgcaattacg atatctttat tacactgaca ggattttaca
    23821 tatttaacgg attatcctca ggcaaaaggg ctggaatacc agcccttttt cgtgttaatc
    23881 ccagctcaga ataactttcc cggactggcc cgaacgcatg gcgtcaaagc ccttctggaa
    23941 atcatcgata gagaaacgat gggtaatgat cggcgagaga tcgaggccag actgaatcag
    24001 cgccgccatc ttgtaccagg tttcaaacat ctcacgaccg taaatgcctt taatgaacaa
    24061 gcctttaaag atcactttgg tccagtcgat agacatgtca gacggtggaa tacccagcat
    24121 cgcaatacga ccgccgtggt tcatggtatc gagcatggta cgaaacgctg gcggcgcgcc
    24181 ggacatttcc agaccgacat caaagccttc ggtcatgccc agttcagcca tcacatcatt
    24241 aaggttttct ttcgcgacgt taaccgcacg ggtgataccc attttgcgcg ccagctcaag
    24301 gcggtattcg ttaacatcgg tgatcaccac attgcgtgca ccaacgtgtt tcgccaccgc
    24361 cgcagccata atacctatcg gacctgcacc agaaaccagc acatcttcgc ccaccagatc
    24421 aaacgacagc gcggtatgca cggcgttacc gaagggatca aaaattgcgg ctaagtcatc
    24481 ggaaatattg tcggggattt tgaaggcgtt gaatgccggg atcaccagat attcggcaaa
    24541 gcagcccggg cgattaacac caacgcctat cgtgttgcgg cacaaatggg tacgaccacc
    24601 acgacagttg cggcaatgac cacaggtgat atggccttcg ccagaaacgc gatcgccgat
    24661 cttgaagcct ttcacttcct gaccaatacc taccacttca ccgacatatt catggcccac
    24721 gaccatcggc accgggatgg ttttttgcga ccactcatcc cagttataga tgtgaacgtc
    24781 agtcccgcag atggctgttt tacggatttt aatcagcaga tcgttatgcc cgagttccgg
    24841 tacaggaacg tcggtcatcc agatgccctc ttccgctttc agtttggata acgctttcat
    24901 ctcacatcct caggcgataa cgcccagttg tttaccaata cgcgtaaatg cttctactgc
    24961 acgcgtaatt tgctcagggg tatgcgccgc agacatctgg gtacgaatac gcgcctgacc
    25021 tttcggaacg accggataga agaaaccggt aacgtaaatg ccctcttttt gcagctcacg
    25081 ggcaaatttc tgcgccacta ccgcatcacc aagcatgacc ggaataatgg cgtgatcggc
    25141 tcccgccagg gtaaagcccg ccgccgacat ttgctcacgg aactgacgcg cgttcgccca
    25201 cagacggtca cgcagttcgc tgcccgcttc gaccatctcc agtactttga tggatgcggc
    25261 aacaatggcc ggtgccagcg agttggagaa cagatacgga cgagaacgct ggcgcagcca
    25321 ctcaaccact tctttgcgcg ccgcagtata accaccagaa gccccgccca gcgctttacc
    25381 aagcgtaccg gtgataatat cgacccggcc catcacatcg cagtattcat gggaaccacg
    25441 accattttca ccgacaaaac cgaccgcgtg ggagtcgtct accataacca gggcatcata
    25501 tttatctgcc agatcgcaaa cgcccttcag gttggcaatc acgccgtcca ttgagaacac
    25561 accatcggtg gcaatcagca catgacgcgc accggcttca cgcgcttctt tcagacgagc
    25621 ttcaagctcc tgcatatcgt tgttggcata gcgatagcgt ttagctttac acagacgcac
    25681 accatcaata atagacgcgt ggttcagtgc gtcggagata atggcgtctt ccgcgcccag
    25741 cagcgtttca aacaggccac cgttagcatc aaagcaggaa gagtagagaa tcgcatcttc
    25801 catccccagg aaggccgcca gtttttgttc aagctcttta tggctgtcct gagtgccgca
    25861 aataaaacgc accgaagcca tgccgaaacc gtgagaatcc attcccgcct ttgccgccgc
    25921 aatcagatca ggatgattcg ccagcccgag atagttgttg gcacaaaagt taatgacgtg
    25981 gcttccatca gccacagtga tatctgcttg ctgcgcagac gtaataatgc gctcttcttt
    26041 aaacaaccct tccgcccgtg cggtttccag atcgttggtt aactgctgat aaaattctcc
    26101 acgcattgcg attctccaga cagggcaaat tccagcacat attacccaaa cttataggtc
    26161 gcgacgagat gacgcgttaa cacttctgca aaattcagga taaataacgt ccatcaatct
    26221 cacttttggc gaatggctga agccaacgcc gtgtagtgat atgatagaag cattcgtgtc
    26281 tgagattgtc tctgactcca taattcgaag gttacagtta tgatcatcgt taccggcggc
    26341 gcgggcttta tcggcagcaa catcgttaag gccctgaatg ataaaggcat caccgatatt
    26401 ctggtggtgg acaacctgaa agacggcacc aagtttgtga acctggtgga tctggatatc
    26461 gcggactata tggataagga agacttcctg atccagatta tagctggcga agagttcggc
    26521 gatgtcgaag caattttcca cgaaggcgca tgctcttcca ccaccgagtg ggacggcaag
    26581 tatatgatgg ataacaacta tcaatactcc aaggagctgc tgcactactg cctggagcgc
    26641 gaaatcccgt tcctgtacgc ttcttccgca gccacctacg gcggacgcac ctccgacttt
    26701 attgaatccc gcgagtacga aaaaccgttg aacgtctacg gttactcaaa attcctgttt
    26761 gatgaatatg ttcgccaaat cctgcctgaa gcgaactcgc agattgttgg cttccgctat
    26821 ttcaacgttt atggaccgcg tgaaggccat aaaggcagca tggcgagcgt cgctttccat
    26881 ctcaacactc agcttaacaa aggtgaatca ccgaagctgt ttgaaggtag cgagaacttc
    26941 aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa acctgtggtt cctggaaaat
    27001 ggcgtttccg gtatcttcaa cctcggtact ggtcgtgcgg aatccttcca ggccgtagca
    27061 gatgctactc tggcttatca caagaaaggc cagatcgaat acattccgtt cccggataag
    27121 ctgaaaggcc gctaccaggc gttcactcag gcagatctga caaatctgcg cgcggcgggt
    27181 tacgacaaac cgttcaaaac cgttgctgaa ggtgtaatgg aatacatggc ctggctgaat
    27241 cgcgacgcat aagagctctg catgaaaata ctggtgatcg gcccgtcttg ggttggcgac
    27301 atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc gctatcccca ggcgataatc
    27361 gatgtgatgg caccggcatg gtgccgtcca ttattatcgc ggatgccgga agttaacgaa
    27421 gctatcccta tgcctctcgg tcacggagcg ctggaaatcg gcgaacgccg caaactgggt
    27481 catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct tacctaactc cttcaaatcc
    27541 gcattagtgc ctttcttcgc gggtattcct catcgcaccg gctggcgcgg cgagatgcgc
    27601 tacggtttac tcaacgatgt acgcgtgctc gataaagaag cctggccgct aatggtggaa
    27661 cgctatatag cgctggccta tgacaaaggc attatgcgca cagcacaaga tctgccgcag
    27721 ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa aatcatatac ctgtaatcaa
    27781 ttttcgcttt catcagaacg tccgatgatt ggcttttgcc cgggtgcgga gtttggtccg
    27841 gcaaaacgct ggccacacta ccactatgcg gaactggcaa agcagctgat tgatgaaggt
    27901 tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag cgggcaatga gattcttgcc
    27961 gctttgaata ccgagcagca ggcatggtgt cggaacctgg cgggggaaac acagcttgat
    28021 caagcggtta tcctgattgc agcctgtaaa gccattgtca ctaacgattc tggcctgatg
    28081 cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt atggtccaag tagcccggac
    28141 ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc tgattaccgg ctatcacaaa
    28201 gtgcgtaaag gtgacgctgc ggagggttat caccagagct tgatcgacat tactccccag
    28261 cgcgtactgg aagaactcaa cgcgctattg ttacaagagg aagcctgacg gatgcgggtt
    28321 ttgatcgtta aaacatcgtc gatgggcgat gttctccata cgttgcccgc actcactgat
    28381 gcccagcagg caatcccagg gattaagttt gactgggtgg tggaagaagg gttcgcacag
    28441 attccttcct ggcacgctgc cgttgagcga gttattcctg tggcaatacg tcgctggcgt
    28501 aaagcctggt tctcggcccc cataaaagct gaacgcaaag cgtttcgtga agcgctacaa
    28561 gcagagaact atgacgcagt tatcgacgct caggggctgg taaaaagcgc ggcactggtg
    28621 acacgtctgg cgcatggcgt aaagcatgga ttggactggc aaaccgctcg cgaaccttta
    28681 gccagcctgt tttacaattg taagcatcat attgcaaaac agcagcacgc cgtagaacgc
    28741 acccgcgaac tgtttgccaa aagtttgggc tatagcaaac cgcaaaccca gggcgattat
    28801 gctatcgcac agcattttct gacgaacctg cctacagatg ctggcgaata tgccgtattt
    28861 cttcatgcga cgacccgtga tgataaacac tggccggaag aacactggcg agaattgatt
    28921 ggtttactgg ctgattcagg aatacggatt aaacttccgt ggggcgcgcc gcatgaggaa
    28981 gaacgggcga aacgactggc ggaaggattt gcttatgttg aagtattgcc gaagatgagt
    29041 ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg tagtgtcggt ggatacgggg
    29101 ttaagccatt taacggcggc actggataga cccaatatca cggtttatgg acctaccgac
    29161 cctggattaa ttggcgggta tgggaagaat cagatggttt gtagggctcc gctaatgaat
    29221 ttaaataatt tagaagcagc ggctgtttat aaaaaaatca cattgattta ataaatagta
    29281 agtgggtatt atacccactt acttagtcaa cgcttttatt attgtggttc ttttttactc
    29341 aaaaacgatg tagacaggaa gaggcacaca atccacgatg ccggtattgg tttagcatgt
    29401 gtaatgacat cactaatgcc aaataacata atagaagcag ggaaaaccaa gagtatatat
    29461 tttttctccc ggatacagta atagatcatt gcaacataga acataataag aacaagaaga
    29521 ccaatttttc ctttcgtgga aagagattcc acaatttcgt tatgcatatg tacatccaga
    29581 aaaggaagaa cactattata aatattatta tctaagctta aagcttttat ctttttggct
    29641 cgctcttcaa gggattgcca tcctaaagta tcgggactac ccttaatacc tgcattaacc
    29701 atagcaaacc gcgcacctac tgatgaattg ctattatttt ttgtagtata ggaaatcaca
    29761 tctgtttgta atgcgttgat ccgtctttca attcccttat tgaatattaa tccgcatact
    29821 agaaataaaa aaaagaaacc actcaatcct ttaaataaaa acactctctg attacgatat
    29881 tgtattaata aaataaccat aatcattagt gggaatgtaa aaatcgccgc cctagtgcca
    29941 gtcattgtta ctgcagcaaa acttaaagca aagttagcat aaaatagaaa ccatttatat
    30001 ttcgattgca ttttgatgaa tacaattgaa gaatataaag tggtgaatgt aatcacataa
    30061 gccgcaatag tcgctgcccc catttgacca atattcccat tcatggcgga gaggggaatg
    30121 cggtctgcat gcaaatatat cccttgataa tatgctctac atagcatgat tatttgaact
    30181 aagatggctg aaataaaatg tagtttttca ttttgcctga atttattttt acttagtaat
    30241 aaaaatgtca acgagctaaa tactaatagt tttccagact caaggtagcc tcgataagca
    30301 tttctatata ctacatcttc tgttttataa aattcatacc atatcaggtt cattg
//
LOCUS       DAZFZC010000060        29546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFZC010000060 DAZFZC010000000
VERSION     DAZFZC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.060"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(8..2497)
                     /locus_tag="ACHKXP_002998"
     CDS             complement(8..2497)
                     /locus_tag="ACHKXP_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFO7647961.1"
                     /translation="MTTNKRHILLNGYVSPENYRSRSNGRSPQVPARDRAVHGISLLN
                     QYSRILNHYDERPRLPPVTDEKGIYVRLISFEQCDLPIDKIDNTYFKLCSLVKSNNRE
                     TAIIYINENDRTKFTKKINDYLNPSKDGIEFPRNHLLIDSIQNIELADITSFWTDKKD
                     LIPDDHGVEKWFELWLKGNKEDVLNIARRLCERINGRLGNTSINFFDTTVVLIRTSLS
                     RLKVCPELISNLKEIRSARDDISVIVNSLPTEQHQWAENVAARITRNNEADVSVCILD
                     TGVNYNNPLLSRFTNSSLAAAWDISWPLFDDYNQRPYNDHGSRQAGLCVYGDFLSVLL
                     NDQDISIPYNIESGRILPPRATNDPNLYGAITTGTSSRLELENPNWRRVYSLAVTAEP
                     NTLGGQPSSWSAEIDKFSFGLEDDIRRLFIISAGNSQPTNLELDYWDSVTLAEIEDPA
                     QSWNALTVGAYTDKTTHTDREYDGWSPFAMSEDIAPSSRSSVSWGWKKHAPYKPDLVE
                     EGGNKLISPSRDEITNTIELSLLTTSGRATNQLFEVNSDTSAACALVSKHAAMLMAQY
                     PEYWPETIRGLLVHTARWTSRMHERYRTERAQGTPKSAKESLLRMVGYGVPNLNRAMH
                     SAENALTLISQSEITPFKRDGSTDPTLNEMHLFSLPWPVEALRLLPPETNVILRITLS
                     YFIEPNPSQKGFRRQYSYQSHGLRFAVIRPNQTLENFRASINRNANNEEYNGPEGDAS
                     GWFLGPQLRVRGSLHSDAWKGSAADLTEMNTIAVYPVGGWWKYRTAQDRYINNVKYSL
                     LVSIDVPDENIDIYSEIQNIIQIDNQIDIEH"
     gene            complement(2506..3492)
                     /locus_tag="ACHKXP_002999"
     CDS             complement(2506..3492)
                     /locus_tag="ACHKXP_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7647962.1"
                     /translation="MATAEQIKALLKSHVDRDDQRFFSIALQVAAKEARQGHHKLAND
                     IKNLVDKNQKTTSSVGLVEKRLTPFVKQPDGDLKGLLEQTNKPVHLQDLVISGSVRER
                     LNQVLLEQKQKDKLSEFGLIPRRKILFTGPPGTGKTMSASVIATELKLPLYTVVLDNL
                     ITRYMGETAAKLRLIFDHIRQTRAVYFFDEFDAIGTQRGAQNDVGEIRRVLNSFLMFV
                     EQDDSESIVLAATNHPELLDRALYRRFDDIIPFTRPEDNLIRNLIEQRLAVFDLGNLF
                     WSEIIDSASGLSAAEITRASEDAAKESVLYNANNITTDLLVKAIKRRQESRQ"
     gene            4174..4533
                     /locus_tag="ACHKXP_003000"
     CDS             4174..4533
                     /locus_tag="ACHKXP_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647963.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGDTNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            complement(4526..5068)
                     /locus_tag="ACHKXP_003001"
     CDS             complement(4526..5068)
                     /locus_tag="ACHKXP_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFO7647964.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSKLHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNEGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPAENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSRINDRLQAIN"
     gene            complement(5189..5374)
                     /locus_tag="ACHKXP_003002"
     CDS             complement(5189..5374)
                     /locus_tag="ACHKXP_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647965.1"
                     /translation="MTPREIALLTIAKLEHGGHQLTQADQREIERSVNADIARRDRFR
                     EMMRAPAYQWKKPAPRR"
     gene            complement(5672..5953)
                     /locus_tag="ACHKXP_003003"
     CDS             complement(5672..5953)
                     /locus_tag="ACHKXP_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7647966.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWMEVMLLCTEDDDREWIKRRRE
                     TCLENVKRPPVKVEEFGDLHKAVTETQHRMGIAQPNGNAFRLNGGKRQR"
     gene            6213..6668
                     /locus_tag="ACHKXP_003004"
     CDS             6213..6668
                     /locus_tag="ACHKXP_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647967.1"
                     /translation="MVFAMSKSNLIAFRIPSELQDEFNRSVLASGGDKTSWLVDAIRM
                     KLGQPEKSIDSRMLGLVERMEKAAASLIAGKPNIPPKPYNETAVIKIIADTIQQGFDN
                     GRVIAERINEAGYQTKAGKAWDKDIYSAWKRQGSNAENLKAVIDCKVSV"
     gene            6748..6969
                     /locus_tag="ACHKXP_003005"
     CDS             6748..6969
                     /locus_tag="ACHKXP_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647968.1"
                     /translation="MKGRTPHMEHQANHFSDVRNEIIAIRGDMHGLERRIMDKIDENR
                     DKASENQKWIIGLLILSMLIPLLIALVTK"
     gene            7490..8104
                     /locus_tag="ACHKXP_003006"
     CDS             7490..8104
                     /locus_tag="ACHKXP_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647969.1"
                     /translation="MSRKDTKELLTICMVYFSIYFCAYMIVPDEFEHGSIIYSITFYF
                     LELTPILAILLAIHIYFSLKKESTNWEKVWRDRGFLTSPKDDAYESDFSDFITNYYSS
                     EQFKNRLSDVWETAMYSIGIILLILTGIAQYVFNIDLSLNPENLISPLIFGEILIFSF
                     GIFLSVVLCSLCKLLTNRYPGEARRARKANPYYLLFPNGDSRDI"
     gene            complement(8123..10228)
                     /locus_tag="ACHKXP_003007"
     CDS             complement(8123..10228)
                     /locus_tag="ACHKXP_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="injection protein"
                     /protein_id="HFO7647970.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGESVEQ
                     AKQAFYAAYPLPSTNEIYRAQSATQNIQHTSSDNSLASGYAKLATQQREGLERSAEQG
                     ASLGAAMRDAITGESRMTPEMERLQNVDLAPELTSLSMDALKARWSQLFGSDASQEKI
                     LQSMGAKLRQDEKGNTIVSLPSGDYALNKPGLSPQDVASFLANALAFTPAGRAGTVLG
                     AIGRSAATDLALQGATSLAGGEDIDPLQTVISAGIGGIGKGLENTASAVSRAVRGDMS
                     PEAKAAVDFASERNLPLMTSDMLKDKTFMQSQAQTLGERVPFFGTGKNRLNQQQAREN
                     LVRTFSDGLGGISDKQLYESATKGQQKFIEAAGKRYNRIIDAMGDTPVDLSNTVKAID
                     NQIAVLSRPGKSQDRAAVKVLQQFKDDITSGPNDLRLARENRTDLRKRFMASSDTVDK
                     DTLQKASDIIYKAYTADMKKAVAKNLGADEAINMARVDRSWSKFNDMMGRTRVQKAIA
                     SGKATPEDVTKLVFSQSPSERSQLYRLLDDNGRQNARAAIVQNAVDKATDPSGNISVE
                     KFINALHRNRKQSATFFKGVHGKELDGVIKYLNDTRHAAKANVQNLNGQQLYGLLVGG
                     GIINAAVLAGMLKTAAFVVPAAGAVGGAAKAYESPVIRNALLRLANTPKGSTAYDRAI
                     STVTQSLTRVAQASQKEAQ"
     gene            complement(10228..11664)
                     /locus_tag="ACHKXP_003008"
     CDS             complement(10228..11664)
                     /locus_tag="ACHKXP_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO7647971.1"
                     /translation="MATWQQGINSGGFLAGIGAQNENAPKASDINTTLGLIRENNELA
                     RSGANNVALTGLRGLAGVADIYKQQQQQERKAAFQKGYADAYASGDREQMRNLITAFP
                     EEFEEVRKGMSYVDDAQRDDYGNLALKAQVASSLGPGAFGRFMMDNEQEMRRLGIPPE
                     TIAEMQVNDPQGFQHFAGNLALFSLGHEKYFDIKDRMEGRDIERGELAETIRSNKAGE
                     GLQAQSIAVSRENSLRTAGGAVPASVKEYQYFNSLSPEQQKTYLRVRGRPAAGGENVV
                     QLADGRTVTVGRKLHGAGANAFYEGIDNEGNMIRVPASSIAAPATSAANAQNYEMKKD
                     LDAISGASIDDLGFMTGITGSSGSFALGADIRSRASGVEERKLYNAAQRIMGKMQNQG
                     IAAARDMGASGINTEAEANRYFQGMPKPDFSSPEALQQSMRDIQQYTDNYNQQYNVNV
                     GKSQRQQSQPAQVSQPAASSNFSSLWGD"
     gene            complement(11669..11842)
                     /locus_tag="ACHKXP_003009"
     CDS             complement(11669..11842)
                     /locus_tag="ACHKXP_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647972.1"
                     /translation="MAISVKPVLISEKQMEAIKKIQEEQRKKSEVGVAPTIHEIARGL
                     MDKALAYTLTGRG"
     gene            complement(12426..15182)
                     /locus_tag="ACHKXP_003010"
     CDS             complement(12426..15182)
                     /locus_tag="ACHKXP_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO7647973.1"
                     /translation="MRNIDLIRQVISASENNWPHVLGCLNINVPDSPRRHAPCPACGG
                     KDRFRFDDNGRGSFICNQCGAGDGLDLIKRVNNCDTTEAALLAADVLGIDYRTTETPE
                     ATSQKREQLETERQRREQERLKRAEKDEQQRRDTFSRQFDDMRRKAVNGKSDYLVAKG
                     VGDFTFPVLPDGSLLLALVDKSGAVTAAQTITSHGEKRLLTGSAKRGAYHAINAPETT
                     QSILIAEGLATALSAHLIRPEALTVAAIDAGNLLYVSQVLRDKFPSAQIIIAADNDHS
                     EGRQNTGRIAAEKAALSVSGWVALPPTDHKADWNDYHQKHGIKCATEAFNKSMYQPQG
                     NGVKQEPQTIEGSDFKVMDTDPLKPRIESREDGIYWVSPRADSQSGEIINNESWLCSP
                     LSVIGTGRDDKDQYLILRWLSFGSETPTTAAIPLADIGEREGWRTLKAGGVNVTTKSS
                     LRAILADWLQRSGSRELWRVAHATGWQCGAYIMPDGEIIGTPENPVLFSGRSSAAAGY
                     TVSGSAKSWRDNVARLAFGNYSMMTGIGAALAAPLIGLVGADGFGIHFYEQSSAGKTT
                     TANVASSLYGNPDLLRLTWYGTALGLANEAAAHNDGLMPLDEVGQGADPVSVSQSAYA
                     LFNGVGKLQGAKDGGNRDLKRWRTVAISTGEMDLETFIATSGRKTKAGQLVRLLNIPL
                     SKAVRFHDYQNGKQHADALKDAYQHHHGAAGREWIKWLADHQQQAIKTVRDCESRWRS
                     LIPSDYGEQVHRVAARFAILEAALLLGEVVTGWDAQTCRDAIQHSYNAWLREFGTGNK
                     EHQQIIEQTEAFLNAYGLSRFAPFPYSPADLPIKDLAGYRQRGEHDESPMIFYTFPAT
                     FEKEIACGFNAKQFAEVLKKAGMLTPPNSGRGYQRKSPRIQGRQINVYVLNYQPGDYN
                     SSEE"
     gene            complement(15195..15797)
                     /locus_tag="ACHKXP_003011"
     CDS             complement(15195..15797)
                     /locus_tag="ACHKXP_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647974.1"
                     /translation="MNNFLTFHAEATPDGVNIMYRSNDGMTERVETVSYIDAVNRLDA
                     GDYDDKPDEGMFIHLAIASGGNQGYFDYTSQHHVIMWRWLIATAFINEMRKENGTVSI
                     IDDSGNHSVVSVYSNGIVAMPLYPVAERLAMANNIEGAMIEKYGVDVGTNNAIIFYSN
                     MFDVEQGTLTSFGREVLADLHNSFIAELNENGIPEAPVTH"
     gene            complement(15790..16011)
                     /locus_tag="ACHKXP_003012"
     CDS             complement(15790..16011)
                     /locus_tag="ACHKXP_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647975.1"
                     /translation="MTNIQLIEAQCRIEQVQTVLGFWLEGASPSNRDKLMIGAVMSLL
                     NGVPEAIQEADELLGKYELQNHSGEAKHE"
     gene            complement(16008..16271)
                     /locus_tag="ACHKXP_003013"
     CDS             complement(16008..16271)
                     /locus_tag="ACHKXP_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acetylcholine receptor subunit beta"
                     /protein_id="HFO7647976.1"
                     /translation="MNINLIYRHPCELEIESLLSREEPYPDTFTLADRTTERLTRART
                     GLVHVMNEILPSVGGEQATVITSWLQKVTSLIDISLIDAESAK"
     gene            complement(16268..16462)
                     /locus_tag="ACHKXP_003014"
     CDS             complement(16268..16462)
                     /locus_tag="ACHKXP_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647977.1"
                     /translation="MNNSINAPRLTSALQLIEQAAAVLVAVSLSAEEMDAADVVDAIK
                     ACSSLVNDARAELVILGGEK"
     gene            complement(16666..16884)
                     /locus_tag="ACHKXP_003015"
     CDS             complement(16666..16884)
                     /locus_tag="ACHKXP_003015"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO7647978.1"
                     /translation="MLSNGGCHMLATTPTQKPQFIWIIAAVRRDCPTITAKIHHIAAE
                     SERDARRSLVRDHVCFFAGRIRMEVAHD"
     gene            complement(16887..17522)
                     /locus_tag="ACHKXP_003016"
                     /pseudo
     CDS             complement(16887..17522)
                     /locus_tag="ACHKXP_003016"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(17516..17698)
                     /locus_tag="ACHKXP_003017"
     CDS             complement(17516..17698)
                     /locus_tag="ACHKXP_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7647979.1"
                     /translation="MSKPTGTPQPQKRYKDAHGALVTVESVSHNRVTFYRDGYQSPCV
                     QPLARFMKEFAEVNKC"
     gene            complement(17691..18524)
                     /locus_tag="ACHKXP_003018"
     CDS             complement(17691..18524)
                     /locus_tag="ACHKXP_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO7647980.1"
                     /translation="MNIEKSRLISEAAPHLNASLSTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGAAYSK
                     IKHSGRPGKDYLLSVGMAKELAMIERNDQGRAIRRYFIQCEEELQRSVPEIAARYRRQ
                     LKARLSAANNFKPMCDALNMARAEMGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVVN
                     V"
     gene            complement(18537..18968)
                     /locus_tag="ACHKXP_003019"
     CDS             complement(18537..18968)
                     /locus_tag="ACHKXP_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7647981.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITHINHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            complement(18968..19171)
                     /locus_tag="ACHKXP_003020"
     CDS             complement(18968..19171)
                     /locus_tag="ACHKXP_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7647982.1"
                     /translation="MANYNSLIRLSEVQRRTGYSKAWIYRLISQGRFPKQVKIGSRAI
                     AFVESEIDEWIEKCILESRDEVA"
     gene            complement(19599..20813)
                     /locus_tag="ACHKXP_003021"
     CDS             complement(19599..20813)
                     /locus_tag="ACHKXP_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7647983.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIITGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRTHIVP
                     LSEQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEHG
                     GNVVHMVFEKHA"
     gene            complement(20979..21054)
                     /locus_tag="ACHKXP_003022"
     tRNA            complement(20979..21054)
                     /locus_tag="ACHKXP_003022"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21019..21021),aa:Thr,seq:cgt)
     gene            complement(21169..22422)
                     /gene="proA"
                     /locus_tag="ACHKXP_003023"
     CDS             complement(21169..22422)
                     /gene="proA"
                     /locus_tag="ACHKXP_003023"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO7647984.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(22434..23537)
                     /gene="proB"
                     /locus_tag="ACHKXP_003024"
     CDS             complement(22434..23537)
                     /gene="proB"
                     /locus_tag="ACHKXP_003024"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO7647985.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            23825..24880
                     /gene="phoE"
                     /locus_tag="ACHKXP_003025"
     CDS             23825..24880
                     /gene="phoE"
                     /locus_tag="ACHKXP_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO7647986.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(24919..25320)
                     /gene="crl"
                     /locus_tag="ACHKXP_003026"
     CDS             complement(24919..25320)
                     /gene="crl"
                     /locus_tag="ACHKXP_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7647987.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(25378..26622)
                     /gene="frsA"
                     /locus_tag="ACHKXP_003027"
     CDS             complement(25378..26622)
                     /gene="frsA"
                     /locus_tag="ACHKXP_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7647988.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(26714..27172)
                     /gene="gpt"
                     /locus_tag="ACHKXP_003028"
     CDS             complement(26714..27172)
                     /gene="gpt"
                     /locus_tag="ACHKXP_003028"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7647989.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            27433..28890
                     /gene="pepD"
                     /locus_tag="ACHKXP_003029"
     CDS             27433..28890
                     /gene="pepD"
                     /locus_tag="ACHKXP_003029"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7647990.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            complement(28947..29468)
                     /gene="prfH"
                     /locus_tag="ACHKXP_003030"
                     /pseudo
     CDS             complement(28947..29468)
                     /gene="prfH"
                     /locus_tag="ACHKXP_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(29467..29546)
                     /locus_tag="ACHKXP_003031"
                     /pseudo
     CDS             complement(29467..29546)
                     /locus_tag="ACHKXP_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7397 a         7325 c         6657 g         8167 t
ORIGIN      
        1 taaaacctta atgttcaata tctatttgat tatcaatttg aataatgttt tgaatctcac
       61 tgtaaatatc aatgttctca tctggtacat ctatgctaac caataaacta tatttaacat
      121 tgttaatata gcgatcctgc gcagtacgat atttccacca tccaccaaca ggatagacag
      181 cgatagtatt catctctgtt aaatctgcag cactgccttt ccaagcatct gagtgtaatg
      241 aacctctaac tctgagttga ggccccagaa accatcctga cgcatctcct tcaggtccat
      301 tgtattcttc attattcgca ttacggttta tcgaagcacg gaaattttca agggtctgat
      361 taggtctaat aactgcaaat ctcaatccat gagattgata cgaatattgt cgtctgaatc
      421 ctttttgact tggattaggt tcaataaaat acgacaatgt gattcttaaa ataacatttg
      481 tttctggtgg tagtaagcga agagcttcta cgggccaagg gagtgaaaac agatgcattt
      541 cattcaatgt aggatcagta gaaccatctc ttttaaatgg ggtgatttcc gactgagata
      601 ttaatgtaag tgcattttcc gcactatgca ttgctcgatt taaattaggt actccataac
      661 caaccatcct taataagctt tctttagccg attttggtgt cccctgtgca cgttctgttc
      721 tatatcgttc gtgcatacga ctagtccatc ttgctgtatg aacaagtaat cccctaatag
      781 tttcaggcca atattctggg tactgagcca ttagcatagc agcatgtttt gatactagag
      841 cacaggctgc gctagtatct gaattaactt caaacaattg atttgttgcc ctgccagagg
      901 ttgtgagcaa agataattca attgtatttg tgatttcatc acggctaggt gatataagtt
      961 tgtttccgcc ttcctctact aaatctggct tatatggggc atgctttttc catccccagg
     1021 ataccgatga ccgagatgac ggtgcaatat cttctgacat agcgaaagga gaccaaccat
     1081 catattcgcg gtctgtatgg gttgttttat cagtatacgc ccctacagtt aatgcattcc
     1141 aagattgagc aggatcttca atttcagcaa gagtcactga atcccaataa tctaattcta
     1201 aatttgtagg ttgagagtta cccgcagaaa ttataaataa tctgcggata tcatcctcta
     1261 aaccaaaact aaacttgtca atctctgcag accatgagga cggttggcct ccaagagtat
     1321 taggctctgc tgtcacagca agcgaataaa ctctgcgcca gttcgggttt tccagctcca
     1381 gacgacttga cgttcctgta gtaatagctc cataaagatt aggatcatta gtagctcttg
     1441 gaggtagtat ccttcctgat tcgatattgt acggaatcga aatgtcctga tcgttcaata
     1501 aaacagacag gaaatctcca taaacacata gtcctgcttg tctggaaccg tggtcattat
     1561 aaggcctttg attataatca tcgaaaagtg gccaagatat gtcccaagca gctgccagtg
     1621 atgagttagt aaatctagat aatagtggat tattgtagtt aacacctgta tctaatatac
     1681 aaacagaaac atcagcttca ttgttacgcg taattcttgc agcaacattt tctgcccact
     1741 gatgctgttc tgtaggtaag gaattaacta taactgatat atcatccctc gctgatctta
     1801 tctcttttaa attagatatt aattcaggac aaacttttaa tctcgataga ctcgtacgga
     1861 taagaacaac agtagtatcg aaaaaattaa tagaagtatt cccgagcctt ccattaattc
     1921 tttcgcataa acgccgagca atatttagca catcctcctt attaccctta agccaaagct
     1981 caaaccactt ttcaacaccg tgatcatccg gaataagatc ttttttatct gtccagaaag
     2041 aagttatatc tgctaactcg atattttgta tgctatcaat taacaaatga tttctaggga
     2101 actcgatacc atccttcgat ggattcaaat agtcatttat ttttttagtg aatttagttc
     2161 tgtcattttc attaatgtat ataatcgcag tttcacgatt atttgattta actaaagaac
     2221 aaagcttgaa ataagtatta tcgattttat ctataggaag atcgcattgt tcaaaactga
     2281 ttagcctaac ataaatccct ttttcatcag taacaggggg aagcctcggt ctttcatcat
     2341 aatgattcaa tatacggcta tactgattta gtaatgatat accatgtacc gctcgatcac
     2401 gagctgggac ttggggacta cgaccattgc tcctagagcg atagttttcg ggggaaacat
     2461 agccgtttaa taaaatatgc ctcttgttgg tagtcatttc atcccttatt gtctactttc
     2521 ttgcctacgc tttatagcct ttactaacaa atcggttgta atattgtttg cattataaag
     2581 cactgattct ttggcagcat cttcacttgc tcgcgtgatc tccgctgcac ttagacctga
     2641 agcactatca atgatctcac tccaaaataa attaccgagg tcaaagacag cgagtctctg
     2701 ttcaataaga ttcctgatta gattatcctc aggccttgtg aacggtataa tatcgtcaaa
     2761 tcgtctatat aaggcgcgat ctaaaagctc tggatgattg gttgcagcta aaactatgct
     2821 ctcagaatca tcctgctcta caaacattaa aaaagaattt aagaccctac gaatttctcc
     2881 aacgtcattc tgagcgccac gctgagttcc tatagcatcg aactcgtcaa aaaaatatac
     2941 agctcttgtt tgccgtatgt ggtcaaaaat taaacgcagc ttagctgcag tttcacccat
     3001 atagcgagtg attagattat ctaagacgac tgtataaagt ggtagcttta actctgtagc
     3061 aatgactgat gcggacattg tcttaccagt accgggagga ccagtgaaaa gaatttttct
     3121 tcttggaata agcccaaact cagaaagttt atctttctgt ttttgttcaa gcagaacctg
     3181 attcaatctt tccctaacgc ttccagaaat caccagatct tgaagatgta ctggcttgtt
     3241 cgtttgctca agtaacccct taagatcacc atcaggctgc ttaacaaatg gtgtaagtcg
     3301 tttttcaact aaacctacag aactcgttgt tttctgattt ttatcaacta agttttttat
     3361 atcattagca agcttatgat gaccttgcct tgcttcctta gctgccacct gcaaagcaat
     3421 agaaaagaaa cgctgatcat cacgatcaac gtggcttttc aataaagctt tgatctgctc
     3481 tgcagtagcc ataacccctc atactccctt gttctcttgt tgcaaagttt actataaatt
     3541 catttagttc gacatcgtta cctacctgct cattacagga aagtaatacc ttcatgagcg
     3601 tatcatcaat gatcaaagtg taatcaaaca cctaggcata gtaccttcac tacgtgaact
     3661 gtatcactca ttgttatgta acctgagtgt ccctttctgg cacttagctc tatcggataa
     3721 tgtatttaag ctgttaattt ataagatttt tcatcactaa actgtctttc atgcaacgca
     3781 tcgataaggt caacacatta cgtttataag gaagtagttt gtttagcgta atgatgcaaa
     3841 tgctgaccaa ttagctttgc cacaattttg ccacacctac aacgcgcaaa attgcacatt
     3901 agtgttgaac aaagcaaaat actgcaatag taacttgatg attttacgag aaaagatggt
     3961 gcgataatag gaacaataca cataggcgat aagctttgtt tttgttgcct tttttattgt
     4021 tcatttttta tagatacccc caaaagtacc cccatatctt tttccgcccc tgaaatttgg
     4081 ttgttattca tacagcatat caggcggtaa cgctctccct ttaacccact tattgtatga
     4141 tagtgctatc ccacaataag gaatcagcca gccatgagaa tgaccagtcg caaaaaagag
     4201 atacttagct actttgaacc ggataacctc gaatgggtga ctggtgagat tggtgcgccg
     4261 ccgttcgatg tgtcgggtgt ggcctacctg cttcatggca tggtgtcgtt cgataagcgt
     4321 caccagctcg aatcaacccg gcgcacactg gaaagcatgg ttgctggtgg actgcttgag
     4381 cgggtgacag tgtatgaatc gcgccagata cggcgcggcg gtgatactaa cgcaacggtc
     4441 gtgcgctacg gtttacccgg tcagtgcgct gttatgcgcg atactggcgg cgctgacaac
     4501 gcaatatcag gtgaatacat gcgggtcagt taatagcctg caatctgtcg ttgatgcggc
     4561 tcaggtcgta aacccggcgt tcaatatcca gcatcaggaa attgtggatc tcggcctgct
     4621 caggattatc agactctctg gcctgtaata gcaattcatt gtaaccatgc aagccgcctg
     4681 agttttccgc cggggtggtg cctttcccac gcaggcagct aatgggctga ttactcgtat
     4741 cgtgtgtttg cttctccagc acaatgacgc aatcccacca gtcgccaaag tcatacgtgt
     4801 aaaccagctt gtcaccctca ttctggagcg ccgcccatag tggcgtgatt gccgcgttac
     4861 ccatgctttc atcctcaccc catacgcgat actcgctacc tggatcacct ttacgccctt
     4921 tctcgaacat atacaggtgt tcgtcattcc agccaaaagc taactggatc acggcatgaa
     4981 gtttactaaa ggggatttgc tctggaacca gcacacgccg ccatataggt ggtttgctgt
     5041 ctcggatggt tatctttagc tggtacatgc aaatcctgaa taaatgcgaa tcggaaggat
     5101 aacgatactg acgtactcac aattctgtaa aggtgaataa aggaaatttg cttaatacgc
     5161 ataagttaca tgtggaataa tcccatatct acctgcgcgg cgctggcttc ttccactggt
     5221 aggcaggtgc tcgcatcatt tcgcggaacc tgtcgcgccg ggctatatcg gcattaactg
     5281 atcgttctat ctcccgttga tctgcctgtg taagctggtg gcctccgtgc tcaagtttgg
     5341 cgatggtcaa taaggctatt tcgcgtggtg tcatacgtca taccctctgg aaggatgaaa
     5401 cacgcccgga ttaaatttca tttttcgcgg gtataaaaaa acgatggggc gggcagtccc
     5461 ggatagtcag ggctgtagag atttgactac cccctcccca ctggattcag gttactgcta
     5521 ttccgttaag cgctgcttac ggggatagct gcgttgtgat tttgaccatt caggtcgccg
     5581 ctatttcctc accgattggg tgctacgcgc gcgcgtactt gtcccgaaat accagaaacc
     5641 gccagccaga ggatgatttc cctctggtgg tctacctttg cctcttgccg ccatttaacc
     5701 ggaaggcgtt accgttcggt tgcgctatcc ccatgcggtg ttgcgtttcg gtaacagctt
     5761 tatgcaggtc gccaaattcc tcaaccttca cgggcgggcg cttcacgttc tccagacacg
     5821 tttcacggcg gcgctttatc cattcccgat catcgtcctc ggtgcataac agcatgactt
     5881 ccatccatcg tgcggcagct ctccggtata gcccttttga ctccagctct tcagctttcc
     5941 tgtcccttat catttctgcc tctcagatta tatagataag caaaaaggtg ttggttcagt
     6001 tggttcagtt ggttcaattt gtaaagatcg ttgtttttat tatgtttttt ctaaaatatt
     6061 gaaccaacat caactcattt tgaaccaaca cagcccagct ctgaaccaac aacacagcga
     6121 tagctttgtg attggtccgt tattttctgg ctgatatttg agcgtataac agtgttatac
     6181 agtggtatac tgtatggata ttactcactg atatggtctt tgcaatgagc aaatctaatc
     6241 tgatcgcgtt ccgcatccca tctgaattgc aggatgagtt taatcggtct gttctcgctt
     6301 ctggagggga taaaacctcc tggcttgttg atgcgattcg catgaagctg ggacagccgg
     6361 aaaagtctat tgattcacga atgcttgggc tggtggagcg catggaaaaa gccgctgcgt
     6421 cgcttattgc cggaaagccg aacatcccac caaagccata taacgaaacg gcagttataa
     6481 agatcattgc cgataccatc caacagggat ttgataatgg gcgtgtgata gctgaacgga
     6541 tcaatgaagc aggctatcaa acaaaagctg gtaaagcgtg ggataaagat atttacagcg
     6601 cgtggaagcg tcagggaagc aatgctgaaa atttaaaagc ggtgattgat tgtaaggtca
     6661 gcgtataaac atgagtgccc attggaaagc tgtttgggcc aataccttta tacaccagca
     6721 ggtggtgacg atgggtgagg atcaggcatg aaaggcagaa cgccacacat ggaacatcaa
     6781 gccaaccact tctcggatgt tcgcaatgag ataattgcca ttcgcggtga tatgcacggc
     6841 ttggagcgtc gaattatgga taagattgat gaaaatagag ataaggccag tgaaaatcaa
     6901 aagtggatca tcggcctgtt gattttatca atgcttatcc cgctacttat cgcattggtc
     6961 acaaaatgaa tatccaatgt gtatgctgtc gatgctacgc gcacgtactt gtcgcaaaat
     7021 atcagacaaa agccagattc agaacgtcgt gataaccacc agcacaacag agaggtcatg
     7081 taacgcgatt tatcacacca gatacatgtg attggtgtac ccgcataaaa catcgtcata
     7141 gcgcgtcctg tgcgcatata cgccgataat caacacatca ccaccactct gtctcgtgta
     7201 gagaacagtt tcatggtatc ccccgccatc gtgtgcgtac tggtcggacg cagttgcaaa
     7261 aaattttgca actgataccc ctcgcgcgcg tactggtcgc gaaattggtg ttttgctgtg
     7321 gcagtgatat cagaaaaatc ttcctgtttc acataaagcg gatatgcgcg tactgtaacg
     7381 gccccatcaa cggggatcgg atttgaaatc ccgacccaag ctatgtacca aatgccatgt
     7441 cttggatata tgatgattgc cattctaaac aacgctaaag ggggttggtg tgagcagaaa
     7501 agatactaaa gaattgctaa ctatctgcat ggtgtacttt tcaatttatt tttgtgcata
     7561 catgatagtc ccagatgagt ttgaacatgg cagtattatt tattccataa cattctattt
     7621 tttagagcta acaccaattt tggctatcct tttggcaata catatttatt tttccttaaa
     7681 aaaagaatcg actaattggg agaaggtatg gagagacaga ggatttctaa catcacctaa
     7741 agatgatgca tatgagagtg atttttctga ctttataaca aactactact ccagcgaaca
     7801 attcaaaaat agactgtctg atgtttggga aacagcaatg tattccatag ggattatatt
     7861 attgatactt actggtattg ctcaatatgt ttttaacatt gacttatcat taaatcctga
     7921 aaatttaata tcgccattga tttttggtga gatattgatc ttctcattcg gaattttctt
     7981 gagtgttgtt ctctgctcat tatgcaaact actgactaac aggtatccag gtgaagcaag
     8041 gagagcaaga aaggcaaatc catattatct tttatttcct aatggagaca gcagagatat
     8101 ctaagccatc catggctacc agttattgag cttctttttg tgatgcctgt gcgactctgg
     8161 tgagcgattg tgtgaccgta ctgatcgctc tgtcatatgc tgtgctacct tttggcgtat
     8221 ttgccaaacg taacaaggca tttcgtataa cagggctttc gtatgccttc gcggctccgc
     8281 ctacggcacc agcagcagga acaacaaacg cagccgtttt tagcatcccc gctaatactg
     8341 ctgcgtttat gatgccacca ccaactaaca atccataaag ctgctgacca tttaagtttt
     8401 gaacgttcgc ttttgccgcg tgtcttgtat cgttgaggta tttaataacg ccgtccagtt
     8461 cctttccatg tacgccttta aagaaagttg ctgattgctt cctgttcctg tgtaacgcat
     8521 taataaactt ctcaacactg atatttccag acggatcagt cgccttatct acagcattct
     8581 gaactatggc tgctcgtgcg ttttgcctac cattgtcatc cagaagcctg taaagctgag
     8641 aacgttctga tgggctttgg ctaaaaacga gttttgttac atcctcaggc gtggccttgc
     8701 cgctggctat tgccttttga acgcgcgttc ttcccatcat gtcattgaat ttagaccatg
     8761 agcgatcaac ccttgccata ttaatggctt cgtctgctcc aagatttttg gctacggctt
     8821 ttttcatatc cgccgtatat gccttgtaga taatatcgct ggctttctgg agcgtatctt
     8881 tatcaacagt gtcagatgac gccataaacc gctttcgaag atcggttctg ttttccctcg
     8941 ccagacgcag gtcatttggt ccgctggtga tatcgtcttt aaattgctgc aagactttta
     9001 ccgcggctct atcctgagat ttacccgggc ggcttaacac agcaatctga ttgtcgatag
     9061 cttttaccgt gtttgagaga tcgacagggg tatcccccat agcgtcaatt atgcggttat
     9121 atcgctttcc tgctgcctca atgaattttt gttgcccttt agtcgcagat tcataaagct
     9181 gtttatcaga aatgccaccc agaccatcgc taaatgttct gactaaattt tctcgtgctt
     9241 gttgttgatt cagccgattc ttaccggttc caaaaaaagg aactctttcg cctaatgtct
     9301 gagcctgact ctgcataaag gttttatctt tcagcatgtc actggtcatt aacggcagat
     9361 ttctttccga tgcaaagtcg acagcagcct ttgcttcagg cgacatatca ccacgaacag
     9421 ccctcgaaac cgcactcgct gtattttcca gccccttacc aataccacca atgccagcag
     9481 aaattaccgt ttgtaacgga tcaatatctt ctccaccagc gaggctggtg gctccctgta
     9541 gtgctaaatc tgtagctgct gatctcccta tggcccccag caccgttccg gccctgcccg
     9601 ctggtgtaaa cgccagcgca ttagccagaa acgatgccac gtcttgcggc gataaacccg
     9661 gcttgttcag agcataatca ccggatggca gggaaacgat agtgttaccc ttctcatcct
     9721 gccttaattt cgctcccatg ctctgaagaa tcttttcctg agatgcgtca gagccgaaaa
     9781 gttgagacca tcgagctttt agagcatcca tgctgagtga ggttaactct ggggcaaggt
     9841 cgacattttg cagtctctcc atctctggag tcattcggct ttcgcctgtt atagcatcgc
     9901 gcatcgcagc cccaaggctg gctccctgtt ctgctgaacg ctcaagcccc tctctttgct
     9961 gagtagctaa ctttgcatac cctgacgcaa gagaattatc agatgatgta tgttgaatat
    10021 tttgagttgc tgattgggct cggtatattt cattcgttga tggtagtgga taggcagcat
    10081 aaaaagcctg cttagcctgc tctacacttt ctccggcttg cggggcaacg acttcattga
    10141 agtattgctc ctgagcctgc gctttttgtt ctggtgctaa cgcctgatac tgtggagaag
    10201 cgataacatc tttccatgct ttagccatta atcaccccat agtgaagaaa agttactgct
    10261 ggctgctggc tgtgatacct gtgcaggttg agattgctgc cgctgagatt taccaacatt
    10321 aacgttatat tgctggttgt aattgtcggt atattgctga atgtcgcgca ttgattgttg
    10381 tagtgcttca gggcttgaga aatcaggctt tggcataccc tgaaaatacc tattggcctc
    10441 tgcttcggtg ttgatgccag atgcccccat atctcgggct gccgcaatcc cctgattttg
    10501 cattttgccc atgattcgct gtgcagcgtt gtatagtttc ctctcctcaa caccagatgc
    10561 ccggctacga atatctgcac caagagcaaa agaacctgaa gaacctgtaa tacctgtcat
    10621 gaagccaaga tcgtcaattg atgcgccaga aattgcatca agatctttct tcatttcgta
    10681 attctgcgca ttagctgccg atgtagccgg agcagcaata gaacttgcgg ggacgcgaat
    10741 catattgccc tcgttgtcta ttccttcgta gaacgcatta gcaccagcgc cgtgaagctt
    10801 cctaccaacc gttaccgttc taccatctgc cagttgcaca acattctccc cgccagcagc
    10861 aggacggcct cgaacacgaa gatatgtctt ttgttgctct ggagacaggc tgttgaaata
    10921 ttgatattct ttaacagatg caggaacagc acctccagca gtgcgcagag agttttcacg
    10981 gctaacagca atgctttgcg cctgtaaacc ttccccagct ttattgctgc gaatcgtctc
    11041 tgccaactcg ccacgctcaa tatcccgacc ctccattcga tctttgatat cgaagtactt
    11101 ctcatgaccg agagaaaaca gcgccagatt acctgcgaaa tgctggaagc cctgcgggtc
    11161 attaacctgc atttcagcaa tagtttctgg agggatacct aaacgacgca tctcctgctc
    11221 attatccatc atgaacctgc caaatgcgcc cggaccaagc gatgaggcta cctgtgcttt
    11281 aagcgccaga ttgccataat catccctttg agcatcatcg acataactca tccctttacg
    11341 gacttcctca aactcttcgg ggaatgctgt aataagatta cgcatctgct ccctgtcgcc
    11401 ggacgcataa gcatctgcat aacctttctg gaatgccgct ttacgctcct gctgttgctg
    11461 ctgcttataa atgtcagcaa ctccagccag accacgaagg cctgtcagag caacgttatt
    11521 tgcacctgat cgagccagtt cattgttttc gcggatcaga ccaagcgttg tgttaatgtc
    11581 gcttgccttt ggcgcattct cgttttgcgc accaatgcca gccagaaaac caccagaatt
    11641 aataccctgt tgccacgtag ccataaattt acccacgtcc agttaaagtg taagccagcg
    11701 ccttatccat taatccccga gcaatttcgt ggatcgttgg cgcaactcct acctctgatt
    11761 ttttacgctg ctcctcctga attttcttta tcgcttccat ttgcttctca cttatcaata
    11821 ctggctttac tgaaatagcc ataatgcccc cttagaactg tataaaacaa cagtcattat
    11881 agtttcataa aacgcaacat tcatcattat gattgcaaaa tatgaaacaa atagaacaaa
    11941 aaaatgccac cgttcagtag ctggtggcat cgttctgtat gtcaggtttt gacaggtttt
    12001 ttatggactc taaaaactat atttatggtg tcgcattact cttcacggca ggcgcttttc
    12061 agttctgccc ttgcttctag ctccattggt aatttgattc gcgatcagct atgcggatgg
    12121 tttgctgaac aggcataaca acttctcagg ggctgttttc tgaactacga aaatttcgta
    12181 ttgctcactt tcaaaacgtc acgcccttgt acacaggatt ttacgaaagt ctcgtaaaac
    12241 actctctgat gccagccagg tgttgtgcgc atcaattcag tcacattgtg ttggttcaaa
    12301 ataccgtgtt gttggttcag tgttggttca ataaaaagaa taaaatcctt ataaaacaat
    12361 actctttaca aattgaacca actgaaccaa ctgaaccaac accttttcta cgcacatgaa
    12421 taagctcatt cttcagatga gttgtaatct cctggctggt aattgagtac ataaacgttt
    12481 atttgtcgtc cctgaatacg tggtgatttt ctttggtatc cacgaccgct gtttggtggg
    12541 gttagcatcc cggctttttt caacacttca gcaaactgtt ttgcgttgaa accacatgcg
    12601 atctcctttt caaatgtggc cgggaatgtg tagaaaatca ttggactttc gtcatgttct
    12661 ccacgctgac ggtatccggc taaatctttg atgggcaaat ctgccgggct gtatggaaat
    12721 ggtgcaaaac ggctaagccc ataggcatta agaaatgcct ccgtttgttc aataatctgc
    12781 tgatgctctt tgttacctgt gccgaactcg cgcaaccatg cgttatagct gtgctgtatc
    12841 gcatcacggc atgtctgagc atcccagcct gttacaacct cgcctaacaa caatgcggcc
    12901 tccagaatgg caaatcgcgc ggctacacgg tgaacctgct caccataatc ggatgggatc
    12961 aggcttctcc atctgctttc gcaatcacga acagttttaa tagcctgttg ctggtggtct
    13021 gccagccatt taatccactc acgcccggct gcgccatggt gatgctggta agcgtctttc
    13081 agtgcgtctg cgtgctgttt tccgttctgg taatcgtgaa agcgtactgc tttactcaac
    13141 ggaatattca gcagtcgcac cagttgcccg gctttggttt ttcgcccgga agtagcgata
    13201 aatgtttcta aatccatttc tccggtgctg attgccactg tacgccagcg ttttagatcc
    13261 cggtttccac catctttagc gccttgcagt ttccctacac cgttaaataa cgcataggca
    13321 gactgtgaaa cactgacagg atcagcgccc tgaccaactt catcaagcgg cattagtccg
    13381 tcattgtggg cggcggcttc atttgccagc cccagcgcgg ttccgtacca cgtcagccgc
    13441 agtaaatcag ggttgccgta taagctgctc gccacgtttg ccgtggtggt cttacctgcg
    13501 ctcgactgct catagaaatg aatgccgaac ccgtcagcgc ccaccagccc aatcaatggc
    13561 gctgccagtg ctgcgccgat cccggtcatc attgaatagt taccaaaggc cagacgcgcc
    13621 acgttatcac gccagctttt cgcgctccct gagacggtat atccggctgc ggctgagctt
    13681 ctcccgctga acagcacagg attttcaggt gttccgatga tttccccatc aggcatgatg
    13741 tacgctccgc attgccagcc agtggcatga gcaacacgcc acaattcacg agagccactg
    13801 cgttgtagcc agtctgccaa tattgctctg aggctgcttt tggtcgtgac atttacccca
    13861 ccagctttaa gtgtgcgcca tccttcacgt tcaccaatat cagccagtgg aatggcagcg
    13921 gttgtcggcg tttcagaacc aaacgacagc cagcgaagaa tgagatactg gtctttatcg
    13981 tctctgccag taccgataac gcttaacgga gaacataacc agctttcgtt attgatgatt
    14041 tctccactct ggctgtcagc tctcggagat acccaataaa taccatcctc gcggctttct
    14101 attcttggtt tcagtgggtc ggtatccata accttaaaat cgcttccctc aatggtctgt
    14161 ggctcctgtt tcacgccatt accctgtggt tggtacattg acttattgaa cgcttctgtg
    14221 gcgcatttaa tgccgtgttt ttggtggtaa tcattccagt ctgctttgtg atctgtcggg
    14281 ggtaatgcca cccatccaga aacagataaa gctgcttttt ctgctgctat tctccccgtg
    14341 ttttgtcttc cttcgctgtg gtcgttatcc gcagcaatga tgatttgtgc tgaaggaaat
    14401 ttatcccgca gtacctggga gacatataac aggttcccgg catcaatcgc cgccactgtc
    14461 agcgcttctg gacgaattaa atgggcagat aatgccgttg ccagcccttc tgcgattaag
    14521 atgctctgtg ttgtttctgg tgcgtttatg gcgtgatatg cccctcgctt tgctgaacct
    14581 gtcaggagtc ttttttcacc atgtgaggta atagtctgtg ctgctgtgac tgcgccggat
    14641 ttatccacca gcgccagcaa cagagatcca tcgggcaaca ctgggaatgt aaaatcacct
    14701 acccctttcg caaccagata atcagatttg ccgtttacag cctttctgcg catgtcatca
    14761 aactgacggg aaaacgtatc ccgtctttgt tgttcgtcct tctctgccct tttcaggcgc
    14821 tcctgttcgc gtcgctggcg ctcggtttcc agttgttccc gtttctggct ggtggcttct
    14881 ggtgtttccg ttgtccggta atcaataccc agaacatcag cggcaagaag cgccgcctct
    14941 gttgtgtcgc agttatttac gcgtttaatt aaatccagcc catcaccagc gccgcactga
    15001 ttacagatga agctaccgcg cccgttgtca tcgaaccgga atcgatcttt gcccccacat
    15061 gcagggcagg gagcatgacg gcgcggagag tcagggacat ttatgttcag gcagcccagc
    15121 acatgaggcc agttgttttc agacgcactg ataacctgac ggataagatc aatgtttcgc
    15181 attctggccc ccgtttagtg cgtcactggt gcttctggga tgccgttttc gtttagttcg
    15241 gcaataaagc tgttgtgaag atcggcaagc acttctcgcc caaacgaagt gagtgttccc
    15301 tgttcgacat cgaacatgtt gctgtaaaaa atgatggcat tatttgttcc gacatcaaca
    15361 ccatatttct cgatcattgc gccctcaatg ttgtttgcca tagcgaggcg ctctgctact
    15421 ggatacagcg gcatggcgac gatgccattg gaataaacag aaaccacgga atgattgcca
    15481 ctgtcatcaa taatgctgac ggtgccgttt tcctttctca tttcattgat gaatgctgtt
    15541 gctatcagcc agcgccacat aatcacgtga tgctgtgatg tgtaatcgaa atatccctgg
    15601 ttgccgccac tggcaatagc gagatgtata aacatgcctt catctggttt atcgtcataa
    15661 tccccggcat ccagacgatt tacggcatca atatatgaga cggtctcaac gcgttctgtc
    15721 atgccatcgt tgctgcggta catgatgttt acgccgtcag gcgttgcttc tgcatggaaa
    15781 gttaagaaat tattcatgtt tcgcctcgcc tgaatgattc tgtaactcat atttgcccaa
    15841 caattcgtcc gcttcctgaa tagcttctgg tacgccattg agcagtgaca taaccgcgcc
    15901 aatcattaac ttgtctctgt tgctggggct ggccccttca agccagaacc ctaaaacagt
    15961 ctgaacctgt tcgatgcgac attgtgcttc aatgagctgg atgttggtca tttcgcactc
    16021 tccgcatcga ttaaactgat atctatcaga gaggtaactt tttgtagcca gctagtgatt
    16081 actgtcgcct gttcccctcc caccgatgga agaatctcat tcatcacatg aaccagacct
    16141 gtgcgcgctc tggtaagccg ttcagtcgtg cgatctgcaa gagtgaatgt gtctggatat
    16201 ggctcttcac ggctcagcaa tgattcaatt tccagctcac acggatgacg ataaattaag
    16261 ttgatattca tttttcaccc ccaagaatta ccagctcggc acgggcatcg ttaaccaaag
    16321 atgagcacgc tttaatcgca tccacgacat cagcagcgtc catttcttca gccgaaagac
    16381 tgacagcaac caggacagcc gctgcttgct cgattaattg aagtgcggac gtaagtcgag
    16441 gggcattaat tgagttattc attggttgcc cccgtttttc ctggcatttc gccaccttcc
    16501 agcaggaaat gaacgtcacg aacggcgaga tccaatatcg cgagtaacgc cttcagtctt
    16561 tcgtctcgtg agggagagtt gctcaccaga atgtcgttaa tcacttctgt cagtgtgatg
    16621 atctggcttg tgcgttcgag gggatccata tgcacatcgt aggttttaat catgagccac
    16681 ctccatgcgg atacgaccag caaaaaagca gacgtgatcg cgcaccagag aacggcgagc
    16741 atcgcgttca gactcggcag caatatgatg aattttggcg gtaattgtcg ggcaatcgcg
    16801 gcgaactgcg gcgataatcc agataaattg cggtttttgg gtaggggtag tagccagcat
    16861 gtggcagcct ccattcgata gcatgtaacg ctaccaccgg aaacgccaat ttcgctggtg
    16921 gtagcccaga cagggttggc gtaaccggct cgaatggata ccggcgcttc cgaagaagcc
    16981 cctgcctgag ccaccattga ttgacaaagg cgctggatta tacaccaacg cccaaaaaat
    17041 gggtgagtca gacctacgac ataaaaaaag acgctcggcg cgtcatatgt cgccattcga
    17101 tttaccagga cgccaatccc ggcacccgtt ttatgaggtg ccttataacc ataaaccgtg
    17161 ttgccgcata acggcaagct cttttttatg gactccgaaa actcgctatt ggaagcacca
    17221 gatgcacagt acatcgcgct catttcgtta acgtcacgcg cgtggacgaa tgtttttgtg
    17281 gagtcactac acgagcggaa gacatcatct acgaccagaa attttctggc gcagccgctg
    17341 accttctcaa tgctaatatc atcgcccgag atttctgata tggttaaggc atccgttccg
    17401 accaccagca aatgataaac tttgcttgta tctgctgtat taatgcctga ctttgccgtc
    17461 atattgctgg attttggcga cataccagcc agtgaaaatg tcttcttctg gacggttagc
    17521 atttgttaac ctccgcgaac tccttcatga aacgcgccag cggttgtacg catggcgatt
    17581 gatacccgtc gcgataaaac gtcactcggt tgtgagacac gctttcgaca gtaacgagtg
    17641 ctccgtgggc atctttgtag cgtttttgtg gttgtggtgt tcctgttggc ttagacattg
    17701 accacctcca gacgcttggc gagccagcgt tgcgacaggc gggtgagttc tcctttgcgc
    17761 tgctcatatt ccatgcccat atcaattaac gtgatattgg tgctttcgag atatgagagg
    17821 tgctcaagct gttctgcgtt catatggtcg cgaggttcgc cagaatagcc atttatccgc
    17881 gcccactgct tagcagttag cccaccaaga acgatacgag aaatcatatt gctctcgttt
    17941 gtgtagtggt gttgctgcgt cgttttaccc atctcggcac gggccatatt cagcgcatcg
    18001 cacattggct taaagttgtt tgcggcactg agacgggctt ttagctggcg acgatagcgg
    18061 gcggcgattt caggcacgct acgctgtaat tcttcctcgc actggatgaa ataacggcgg
    18121 atagcgcgac cctgatcatt gcgttcgatc attgccagtt ctttcgccat tccgacactt
    18181 aacagatagt ctttgccggg tctgccagag tgctttatct tgctgtaagc cgcgccattc
    18241 gggcttttcc ccaaatttgg ggaaattgtt ttatggactg agtaatcgtg cccaatggtg
    18301 aagtcatatt cagagatgcg atcagtgatc catgtagaga agtcttttcc cacgcccaac
    18361 gctttgtgta acgcttttgc gctaacaata ttggtttcac gcccaccgat atgaccagga
    18421 ataaccggga caattgcggc aaattcatta ccgttaattg tgctcagaga ggcgttcaga
    18481 tgaggggcgg cctcagaaat taatctgctt ttttcgatgt tcattttcag tctccgttag
    18541 gcggcgttaa acgcgtccgg gtagagtttc agaatgtcgg caatttcctg ctgagaaagt
    18601 ccgttgtgat catttatgtt cgcaagatgg ttaatatgag tgatcacttt cagaacatca
    18661 ccacggcagg agaaacggta gcgaaggtgt gttccgatac catcaggatt cttttcgtcg
    18721 atacgctcca gagtgattcc aagctggcgt tctaactctg ttgcatagtt acgaccagaa
    18781 gacagacggc agtagcgaag aatgtcattt tctgtccatc cctctatacc agtacgcagc
    18841 atatagactc tggcacgatg ctttttcggt gtgcgctttg gtgcttgaag ggattttgta
    18901 aggggcgtaa catcagatac acgaatatct gaattagccg cctgtaatgg gcggtttttc
    18961 ttttccatca ggccacctcg tctctagatt ctaatataca cttctcaatc cattcatcta
    19021 tttcagactc aacaaaagca attgcccgac ttccaatttt tacctgttta gggaacctcc
    19081 cctgactaat aagacgataa atccatgctt tgctataacc agttcgcctt tgaacttcag
    19141 agagacgaat taatgaattg taattagcca tttattaata tcctgtacta atgacgcaat
    19201 aaccccctcc tgcaacgaat ggcgagagtt ttatttgcgt caaatgtcga atacgccaaa
    19261 gaccacaata tgaaaaacat attgcggtct tttataaaag atagtcagat tgttttggtt
    19321 tgtttgctcc acaagggtag agctaccctg catttcggca ggatcacgct attaggcgtg
    19381 acatccagta gttttaaaga acaattgcgc tttgtcagcg ccaaagcctg ttaaagaaca
    19441 ttccgctttt cagcagcgta gaaggctcaa tacaccttcg tttataaaag agcgattgcc
    19501 cttctccagg gctaactgtt aaatagcaat cctctgtgag tttttatcaa tctactcccc
    19561 tatactttag taaacctctg tatacttgtc cagttgtttc atgcgtgttt ttcaaacacc
    19621 atatgcacca catttccgcc atgctcgaga gaatccatat agtcggcata ccattgaagc
    19681 atctcccgcc gtccatccag atattgcgca tggttgtatg tgccacgaat tgagttctta
    19741 tcaacgtgag cgagctgcgt ttctatccac gcggtgttat agccctgctc atgcaaaatc
    19801 gtgctcatag tgtgtcggaa cccatgacca gttacacgtc cagcgtagcc aatgcgctta
    19861 aacacttggt ttatgctggc ctcactcatt gttttccttg gatcattgcg cccgggaaac
    19921 ataagagggt aattgcctgt tatttctcta atcttcccaa taagcgaaag agcctgctca
    19981 gacaatggca caatatgagt ccgccgcatt ttcatacgtg aagcgggtat ctcccagata
    20041 gccttgttga aatcaatttc atcccataat gcgccacgta gttcgccagt ccgcaagccg
    20101 gtgataatca gtagacgagc cgccataaca accaatgcgc ttcctgagta actggacaat
    20161 gccttgaaaa aatcaggtaa ttctttggct gtaaggaaag ggtaatgatt agattcatgg
    20221 ccttgcatcg cgctagtaag gtctggtgca gggttatact ctgcgcgacc agtgactatt
    20281 gcatagcgga aaacttcccc gcaccgttgc ctcacttttt tggctttttc tgtagcaccg
    20341 cgcccctcga tgcgacgcag cacattcaga agttcaagtg gtttgatttc ggcgatgggt
    20401 tttttgccaa tgtaagggaa cacatctttg ttgaaggctt cgaggatgtc tgaagcataa
    20461 ccagcagacc atttttttaa tttgctgctg tgccactcaa gggcaatatc tttgaaggtg
    20521 ttgtttaact gcgtttcccg ggcaatcttt tcctctcgtt tcgcttccat cggatcgata
    20581 ccaccagcga tacctctttt cgcatcttca cgttttgccc tagcatcggc tagagtaacc
    20641 tcaggataaa cacctagtgc caatagcttc tctttaccag ctacacgata tttaagtcgc
    20701 cagtatttac ctccattagg ttttaccaag agatacaaac cgccaccatc ggccagcttg
    20761 taagccttct cttttggctt ggctgtgtct atttgacggg cattgagttt cacttggggg
    20821 tacctccact aaaccgaaca gcaaataccc ccataagtac ccccatctga ccgtagattt
    20881 tgagggactt tagtagacgt caaaagacga acggggcttt aatacgcgga ttataagggg
    20941 ttttgaaaga ctttagtaga cttggggaga tgtttgaatg gtgccgataa taggagtcga
    21001 acctacgacc ttcgcattac gaatgcgctg ctctaccaac tgagctatat cggccctgaa
    21061 aggacatgtt cacgaacgtg aatcacggtg gacaaggtta aaactaaccg ggcgatgcgt
    21121 caatggcctt gtgaatcaaa tggctacttt tgcatcaccc ggttttattt acgcacgaat
    21181 ggtgtaatca ccaatgccga tccacttgta agtggtcagt gcttccagcc ccattgggcc
    21241 acgcgcgtgg agtttttgtg tgcttaccgc cacttccgca cccagtccaa actggccgcc
    21301 gtcggtaaaa cgcgtagagg cgttaacgta aacagcggac gaatccactt cgttaacaaa
    21361 acgctgggcg ttgcgcatat cgcgggtcag gatcgcatcg gagtgttgtg tgccgtgttc
    21421 acgaatatgg gcgatggcat cgtcaagatc actgacgatt ttgacgttca aatctaatga
    21481 cagaaactca tcgtcatact cttccgcttt aacagccacc accttcgcgg ggcctgtctg
    21541 caactgcgcc agcgcagctg catctgcgtg taatgccacg ccgctttcct ccatttgttt
    21601 gcttaatgcg ggcaggaagc tatcggcgat gtttttattc accagcaacg tttctaccgt
    21661 attacatgtg ctcggacgct gagttttcgc gttgacgatc acttttaatg cttcagcaat
    21721 ctctacactt tcatcaacat aaatatggca tacgcctata ccacctgtga tcaccgggat
    21781 cgtcgactgt tcgcggcaca gtttatgcaa accagcgcca ccacgcggga tcagcatgtc
    21841 gatgtattta tccatacgca gcatttcact gaccagcgca cggtcaggat tatcaatcgc
    21901 ctgcacggca cccgccggta agccgcagga tttcagggcg tcctgaatca ccgccaccgt
    21961 tgcagcgtta gtgcgacacg tttctttgcc accgcgcagg atcaccgcat taccggtttt
    22021 caggcacagc gaagcgacat caaccgtcac gttcgggcgc gcttcataaa tcacgccaat
    22081 aacccccagc ggtacgcgac gacgctcaag acgcaggccg ctgtccagta cgccgccatc
    22141 gattacctgc cccaccggat cggcgaggtt gcacacctga cgtacatcgt cggcaatgcc
    22201 tttcagccgt gcgggcgtca gtgccagacg gtcaagcatc gcttcgctaa ggccattggc
    22261 tcgcgcgtca gcaacatcct gggcgttagc gttgaggatg atttcgcttt gtgcttccag
    22321 ttcatcggcg attttttcca gcacgcgatt tttttcgcgg ctggagagtt gcgctaattt
    22381 atacgaggct tgcttcgcgg caatgcccat ttgttccagc atcagcctgc tccttaacgg
    22441 gtaatcatgt catcacggtg aacggcaacc gggccgtatt catatcccag tattgcatca
    22501 atttcttgcg agtggtgtcc ggcaatacgg cgtaatgcat cgctgttgta acgactgacg
    22561 ccgtgggcga tatcgcggcc ttcgaggttg caaatgcgga tgacttcacc acgcgagaaa
    22621 ttgccagtca cgcttttaat gcctttcggc aacagggagc tgccgcgttc cagaatggcg
    22681 gcagttgccc cttcatctac cgtgatttca cccgccggcg gcgcaccgaa aatccagcgt
    22741 ttacggtttt caagcggagt cgcctgggca tggaacagcg taccgacgga aatgccttcc
    22801 atcacatcac caataacgcc cggcttgctg cccgcggcaa taatggtgtc gatacccgca
    22861 cggcaagcca cgtcagcggc ctgcaatttg gtactcatgc cgccagttcc gaggcctgaa
    22921 acgctgtcac cggcaatcgc gcgcagtgcg tcatcaatgc cgtaaacatc tttaatcagt
    22981 tctgcctgcg gattgctgcg cgggtcagcg gtatacaaac ctttttgatc ggtcagcagc
    23041 aacagtttat cggcacccgc aagaatcgcc gccagcgcag aaaggttatc gttatcgccg
    23101 accttaatct ctgccgtagc gacagcatcg ttctcattga ttaccggaac gatattgtta
    23161 tcgagcaacg ctcgcagggt gtcgcgggcg ttcaggaagc gttcacggtc ttccatatca
    23221 gcacgggtca gcagcatttg cccgacgtga atgccataaa tcgaaaacag ctgttcccac
    23281 agttgaatca gtcgactctg ccctaccgcc gccagcagtt gtttcgaggc gatggtcgct
    23341 ggcagttccg ggtaacccag gtgctcacgt ccggcggcga tcgcgcccga cgtcacaata
    23401 acaatccgat gcccggcggc atgtaactgc gcgcactggc gaacaagttc aacgatatgg
    23461 gcacggttca gacggcgcga tccgcctgtt agcacactgg tgccgagttt taccaccagc
    23521 gtctggctgt cactcatgat tctctgccat tcaattttag gaaaaatgat atcaaacgaa
    23581 cgttttagca ggactgtcgt cggttgccaa ccatctgcga gcaaagcatg gcgttttgtt
    23641 gcgcgggatc agcaagccta gcggcagttg tttacgcttt tattacagat ttaataaatt
    23701 accacatttt aagaatatta ttaatctgta atatatcttt aacaatctca ggttaaaaac
    23761 tttcctgttt tcaacgggac tctcccgctg aatattcgcg cgttaattaa aatcaggaat
    23821 gaaaatgaaa aagagcactc tggcattagt ggtgatgggc attgtggcat ctgcatctgt
    23881 acaggctgca gaaatatata ataaagacgg taataaactg gatgtctatg gcaaagttaa
    23941 agccatgcat tatatgagtg ataacgccag taaagatggc gaccagagtt atatccgttt
    24001 tggttttaaa ggcgaaacac aaattaacga tcaactgact ggttatggtc gttgggaagc
    24061 agagtttgcc ggtaataaag cagagagtga tactgcacag caaaaaacgc gtctcgcttt
    24121 tgccgggttg aaatataaag atttgggttc tttcgattat ggtcgtaacc tgggggcgtt
    24181 gtatgacgtg gaagcctgga ccgatatgtt cccggaattt ggtggcgatt cctcggcgca
    24241 gaccgacaac tttatgacca aacgcgccag cggtctggcg acgtatcgga acaccgactt
    24301 cttcggcgtt atcgatggcc tgaacttaac cctgcaatat caagggaaaa acgaaaaccg
    24361 cgacgttaaa aagcaaaacg gcgatggctt cggtacgtca ttgacatatg actttggcgg
    24421 cagcgatttc gccattagtg gggcctatac caactcagat cgcaccaacg agcagaacct
    24481 gcaaagccgt ggcacaggca agcgtgcaga agcatgggca acaggtctga aatacgatgc
    24541 caataatatt tatctggcaa ctttctattc tgaaacacgc aaaatgacgc caataactgg
    24601 cggctttgcc aataagacac agaactttga agcggtcgct caataccagt ttgactttgg
    24661 tctgcgtcca tcgctgggtt atgtcttatc gaaagggaaa gatattgaag gtatcggtga
    24721 tgaagatctg gtcaattata tcgacgtcgg tgctacgtat tatttcaaca aaaatatgtc
    24781 agcgtttgtt gattataaaa tcaaccaact ggatagcgat aacaaattga atattaataa
    24841 tgatgatatt gtcgcggttg gcatgaccta tcagttttaa tggatattgc cggatgtgat
    24901 acatccggca catttcactc acgccgttaa cttcaccggt tcatcacgaa aatcatccgc
    24961 cggttccagc ttcagattca gcgtcgtcag cagttcacgc agcttctcgt gaaactcacg
    25021 tagggtgtgc tccagtcgtt caaccacttc agtgtctttt accggaacac tcttccagtc
    25081 gcctgcttta tcgaacagac caaactggta actgtaggta aaacgggatt cctgcgcttc
    25141 aagctccatc caccagcccc agaactcacg cacttccggt gccggtttca cgttgacgca
    25201 tacagccaga caatcgaaaa agaatcgatt atcttcgcac ttaccttcac gaatatacgg
    25261 gcctagtgcg gtaaattttt tgatcaatct gctcttcggg tgtccactcg gtaacgtcat
    25321 tgcaatctcc tgttgtgatg caactgtttt accaaattgg caaattttag caaattttta
    25381 acacaaacgt ttttcgatcc agtctgtgat ttcctgaaga cctttgtcaa aattccgata
    25441 caccgggtta aatgggatct ctaataattt accgtcagca gatgatgagg tgattaagcg
    25501 tgagtcctct tccgggctga acggatcgtt cttccagtag cccgataaca ttggcgttgg
    25561 gcagcgacgt cccagcaatc cttgcacttt taatgaatag cgattcagct ccacgcgcaa
    25621 cgcttcgtcg gaagcatcat gcatccccaa acgactcgcc agaacgtcaa gatacatttc
    25681 cggcacctgt tgctggcact taaaatcact caacagggta tgaactaccg gaccaagaca
    25741 ggcaaccgct ttcagacgcg gcgattcaag gtatgccaga cgcacggcaa cgttagcgcc
    25801 gaaacggaaa ccaaaggccg cgacgcgagt gtgatccacc cacggtacgt taggcagcgc
    25861 ctttaagacg tgctgatgca acaggctgga gtcctgggtc agcttccatt ttgaagaaaa
    25921 gcccaccgac ggcatatcaa tagtcagcat cgcaatgccg cgcggcgcaa aataacgttc
    25981 gtacaggctg taatagtccg tctgcatcgc atccagacca ccacacatta atactgtcgg
    26041 gaacgggcca tcgcctttcg gcatatgcaa aaagccggtg atgggcgcac cgccgggtac
    26101 ggtaaactcc atctgccgca tcgtgcccgg cagacgctga gcggcctctt cataggcgcg
    26161 gtttgacaaa gcctgcgctt gctcggccag gtcatctcct ttcagatgag gataggcagc
    26221 aatgttatac aacgtagccg catgcagcca gtgacgacca ctgagttgcg gatcttcttc
    26281 cgcacaggct ttttgttgcc acaccatcgc ctgggtggcc cactcataaa tccagttgcc
    26341 accacggtag ccaatcacag tatcgtataa atcatcgtcg gtacgttcag catcgctcat
    26401 cacaatacgt gcctggacgt cgaggatttc acgtgggtca ataccgcgcc agatccacat
    26461 cagacggtta atcatgcgat accagtgagg gatggtttta ccatcaagag ccgactgcac
    26521 aggcggttgt gcgccgtgat taaagcggcg gactagcgtc gaggtttcag gatgtttaaa
    26581 gcggggtttg aacagggttt cgctcaggtt tgcctgtgtc atggatgcag cctccagaat
    26641 acttactgga aactattgta acccgcctga agttaaaaag aacaacgccc ggcagtgcca
    26701 ggcgttgaaa agattagcga ccggagattg gcgggacgaa tacgacgccc atatcccacg
    26761 gctgttcaat ccaggtatct tgcgggatat caacaacata gtcatcaacc agcggacgac
    26821 cagccggttt tgcgaagatg gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa
    26881 ccgcagtacc accggtatcc accaggtcat caataacgat gaagccttcg ccatcgcctt
    26941 ctgcgcgttt cagcacttta agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa
    27001 cggtatcgac atgacgaata cccagttcac gcgccagtaa cgcacccggt accagaccgc
    27061 cacggcttac ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt
    27121 tacgtgcatg gatctgcaac atgtcccagg tgacgatgta tttttcgctc atgtgaagtg
    27181 tcccagcctg tttatctacg gcttaaaaag tgttcgaggg gaaaataggt tgcgcgagat
    27241 tatagagatc tggcgcacta aaaaccagta tttcacatga gtccgcgtct ttttacgcac
    27301 tgcctctccc tgacgcggga taaagtggta ttctcaaaca tatctcgcaa gcctgtcttg
    27361 tgttgacaac attttctgct aaccctgtga cctgcaatac tgttttgcgg gtgatcgaca
    27421 aggagactta acgtgtctga actgtctcaa ttatctccac agccgctgtg ggatattttt
    27481 gccaaaatct gttctattcc tcacccgtcc tatcatgaag agcaactcgc tgaatacatt
    27541 gttggttggg caaaagagaa aggtttccat gtcgaacgcg atcaggtagg taatatcctg
    27601 attcgtaaac ctgccaccgc aggtatggaa aatcgtaaac cggtcgtctt gcaggcccac
    27661 ctcgatatgg tgccgcagaa aaataacgac accgtgcatg acttcacgaa agatcctatc
    27721 cagccttata ttgatggcga atgggttaaa gcgcgcggca ccacgctggg tgcagataac
    27781 ggcattggta tggcgtctgc gctggcggtt ctggctgacg aaaacgtggt tcacggcccg
    27841 ctggaagtgc tgctgaccat gaccgaagaa gccggtatgg acggtgcgtt cggcttacag
    27901 agcaactggt tgcaggctga tattctgatt aacaccgact ccgaagaaga aggtgaaatc
    27961 tacatgggtt gcgcgggggg tatcgacttc acctccaacc tgcatttgga tcgtgaagcg
    28021 gttccagctg gctttgaaac cttcaagtta accttaaaag gtctaaaagg cggtcactcc
    28081 ggcggtgaaa ttcacgttgg cctgggtaat gccaacaaac tgctggtgcg cttcctggcg
    28141 ggtcatgcgg aagagctgga cctgcgcctt atcgatttca acggtggcac actgcgtaac
    28201 gccatcccgc gtgaagcctt tgcgaccatt gctgtcgcag ctgataaagt cgacgccctg
    28261 aaatctctgg tgaataccta tcaggacatc ctgaaaaacg agctggcaga gaaagagaag
    28321 aatctggcct tgttgctgga ctctgtagcg aacgataaag ctgctctgat tgcgaaatct
    28381 cgcgatacct ttattcgtct gctgaacgcc accccgaacg gtgtgatccg caattccgac
    28441 gtggcaaaag gtgtggtcga aacctccctg aacgtcggtg tggtgaccat gactgacaat
    28501 aacgtagaaa ttcactgcct gatccgttca ctgatcgaca gcggtaaaga ctacgtggtg
    28561 agcatgctgg attcgctggg taaactggct ggcgcgaaaa ccgaagcgaa aggcgcatat
    28621 cctggctggc agccggacgc taattctccg gtgatgcatc tggtacgtga aacctatcag
    28681 cgcctgttca acaagacgcc gaacatccag attatccacg cgggcctgga atgtggtctg
    28741 tttaaaaaac cgtatccgga aatggacatg gtttctatcg ggccaactat caccggtcca
    28801 cactctccgg atgagcaagt tcacatcaaa agcgtaggtc attactggac actgctgact
    28861 gaactgctga aagaaattcc ggcgaagtaa ttatttgatt tgctgccgga tggcgtttaa
    28921 tcgccttccg gcagtttcat ccttcattat ccttcgataa aagccatccc tgtaaatgtc
    28981 cgtcgcgggt tgccacgttc aatctggtga tggaacattc gccgctgcga tttcagcgcc
    29041 gcgctatttt cctgttgctg ctgctccagc ttccaggcaa tcagcaatcg cgccagccgt
    29101 ttgttagcat gctggctgcg ctctgactga accttcacgc taataccgga tgccagatgc
    29161 gtggcgcgta ccgccgagtc ggttttattg acatgttgac cgcccggccc cgacgaacgc
    29221 agcgtctcat aacggattgc atccgattgt tccttctcat cagcggtaaa acgcccaatg
    29281 cccagaaacc agtttttgcg cccatgatga ggccgatacg gactcggaca aatccactga
    29341 atagtgccac accagctttc gcttaatgcc catgcgttat cgccatccag agaaaccagc
    29401 gccgaacgca gcgtgtcaga gtagcggccc gtttctgttt ccagcaccgt tacagcgacg
    29461 tcttgtcggg taaaaacgac caacagagaa gaacgctgcg ccaatcgggt agccgcgtcc
    29521 ggggtgtaaa tctggcatcc ccacta
//
LOCUS       DAZFZC010000061        29395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFZC010000061 DAZFZC010000000
VERSION     DAZFZC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.061"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..259
                     /locus_tag="ACHKXP_003032"
     CDS             <1..259
                     /locus_tag="ACHKXP_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7647991.1"
                     /translation="FSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSV
                     APGNAVPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            271..1383
                     /locus_tag="ACHKXP_003033"
     CDS             271..1383
                     /locus_tag="ACHKXP_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7647992.1"
                     /translation="MQRKGNKLLIQLCSAILLFFTTSWYALANECYIESNAEGPYDMV
                     INPTPFSIQTRMVDAPEEIAFATWDSHINLRGDKIGCKSLGSNTSSVHFLNTADASLL
                     STYTTSRGDALLKTTVPGIVYSVELVCKSCGAAEELDLYLPAQSGADNHTDNASAKWA
                     YEYSDDSWYLRFRFFWTPEFKPQTGVSEGTAMPGKIASWYIGNNDQPWINFFVDTSSV
                     HFYVDEPTCQTVALAQDQGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TVKLKAANKPSDGTLVGKTTGSASGVAVKVNSTYDNSKVLLKADGSNAVDYNFAIWSN
                     NLLNFPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            1519..2313
                     /gene="panB"
                     /locus_tag="ACHKXP_003034"
     CDS             1519..2313
                     /gene="panB"
                     /locus_tag="ACHKXP_003034"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO7647993.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            2325..3176
                     /gene="panC"
                     /locus_tag="ACHKXP_003035"
     CDS             2325..3176
                     /gene="panC"
                     /locus_tag="ACHKXP_003035"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO7647994.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(3468..4364)
                     /gene="rpnC"
                     /locus_tag="ACHKXP_003036"
     CDS             complement(3468..4364)
                     /gene="rpnC"
                     /locus_tag="ACHKXP_003036"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO7647995.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKLDKKMAFRIMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSESQLVAMRNYMLQR
                     GHTEQADLFYGVLRDRETGGESMVTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            4638..5018
                     /gene="panD"
                     /locus_tag="ACHKXP_003037"
     CDS             4638..5018
                     /gene="panD"
                     /locus_tag="ACHKXP_003037"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO7647996.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(5022..6251)
                     /gene="yadE"
                     /locus_tag="ACHKXP_003038"
     CDS             complement(5022..6251)
                     /gene="yadE"
                     /locus_tag="ACHKXP_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7647997.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLSVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDNAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(6315..6755)
                     /gene="yadI"
                     /locus_tag="ACHKXP_003039"
     CDS             complement(6315..6755)
                     /gene="yadI"
                     /locus_tag="ACHKXP_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7647998.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(6860..7630)
                     /gene="yadH"
                     /locus_tag="ACHKXP_003040"
     CDS             complement(6860..7630)
                     /gene="yadH"
                     /locus_tag="ACHKXP_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7647999.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(7627..8553)
                     /gene="yadG"
                     /locus_tag="ACHKXP_003041"
     CDS             complement(7627..8553)
                     /gene="yadG"
                     /locus_tag="ACHKXP_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7648000.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGLNSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            8662..9324
                     /gene="can"
                     /locus_tag="ACHKXP_003042"
     CDS             8662..9324
                     /gene="can"
                     /locus_tag="ACHKXP_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO7648001.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(9365..9901)
                     /gene="hpt"
                     /locus_tag="ACHKXP_003043"
     CDS             complement(9365..9901)
                     /gene="hpt"
                     /locus_tag="ACHKXP_003043"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO7648002.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            10107..12497
                     /gene="gcd"
                     /locus_tag="ACHKXP_003044"
     CDS             10107..12497
                     /gene="gcd"
                     /locus_tag="ACHKXP_003044"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO7648003.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYS
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMVLPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            12536..12634
                     /locus_tag="ACHKXP_003045"
                     /pseudo
     CDS             12536..12634
                     /locus_tag="ACHKXP_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12699..14240)
                     /gene="cueO"
                     /locus_tag="ACHKXP_003046"
     CDS             complement(12699..14240)
                     /gene="cueO"
                     /locus_tag="ACHKXP_003046"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO7648004.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGTQFR
                     ILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDTGMM
                     LGFTV"
     gene            14406..14753
                     /gene="yacC"
                     /locus_tag="ACHKXP_003047"
     CDS             14406..14753
                     /gene="yacC"
                     /locus_tag="ACHKXP_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO7648005.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            14859..15725
                     /gene="speE"
                     /locus_tag="ACHKXP_003048"
     CDS             14859..15725
                     /gene="speE"
                     /locus_tag="ACHKXP_003048"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO7648006.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            15741..16535
                     /gene="speD"
                     /locus_tag="ACHKXP_003049"
     CDS             15741..16535
                     /gene="speD"
                     /locus_tag="ACHKXP_003049"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO7648007.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(16573..16935)
                     /gene="yacL"
                     /locus_tag="ACHKXP_003050"
     CDS             complement(16573..16935)
                     /gene="yacL"
                     /locus_tag="ACHKXP_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO7648008.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(17111..19708)
                     /gene="acnB"
                     /locus_tag="ACHKXP_003051"
     CDS             complement(17111..19708)
                     /gene="acnB"
                     /locus_tag="ACHKXP_003051"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO7648009.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            20063..21916
                     /gene="yacH"
                     /locus_tag="ACHKXP_003052"
     CDS             20063..21916
                     /gene="yacH"
                     /locus_tag="ACHKXP_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO7648010.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTFPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQSQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAANQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFHDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPKIQQRREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(21987..23411)
                     /gene="lpdA"
                     /locus_tag="ACHKXP_003053"
     CDS             complement(21987..23411)
                     /gene="lpdA"
                     /locus_tag="ACHKXP_003053"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO7648011.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(23736..25628)
                     /gene="aceF"
                     /locus_tag="ACHKXP_003054"
     CDS             complement(23736..25628)
                     /gene="aceF"
                     /locus_tag="ACHKXP_003054"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO7648012.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSRSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(25643..28306)
                     /gene="aceE"
                     /locus_tag="ACHKXP_003055"
     CDS             complement(25643..28306)
                     /gene="aceE"
                     /locus_tag="ACHKXP_003055"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO7648013.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(28467..29231)
                     /gene="pdhR"
                     /locus_tag="ACHKXP_003056"
     CDS             complement(28467..29231)
                     /gene="pdhR"
                     /locus_tag="ACHKXP_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO7648014.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         7064 a         7945 c         7258 g         7128 t
ORIGIN      
        1 ttttagtaat gaatatgaac agcaggctac cggggcgaaa aacgtcggta ttgtgatatt
       61 ttctgcccaa ccgaatcagc agacatttaa cgtgcgagga acggatggtt cctcaacagc
      121 aatttattcg gtcgcgcctg gcaatgctgt tccctcaacc tggacctttt attcccgtat
      181 gcagcgggtg aataatgctc tcccgcccga atcgggaatg gtgagaagcc aggtgattgt
      241 taacgtatct tacgaataat aaggataatt atgcaaagga aaggcaataa actgttgatt
      301 cagttatgca gtgcgatact gctatttttt accacatcct ggtatgcatt ggcgaatgaa
      361 tgttatatag agagcaatgc tgaagggcct tatgatatgg tgataaaccc aacacccttc
      421 agtatacaga cacgaatggt tgatgcacca gaggaaatag ccttcgcgac ctgggattct
      481 catattaatc ttcggggcga taagattggt tgtaaatcct taggcagtaa cacgagctct
      541 gtgcattttc ttaatactgc tgatgccagt ctcttatcaa cctatactac cagccgtggc
      601 gatgctttat tgaaaacaac cgttcccggt attgtttatt cagtagaatt agtatgtaaa
      661 agttgtggag ccgcggagga acttgatttg tatcttccag cacaaagtgg tgctgataat
      721 catacagata acgccagcgc aaagtgggct tatgagtata gtgacgatag ctggtattta
      781 cgatttcgtt ttttctggac cccggaattt aaacctcaaa ctggggtttc agaaggaact
      841 gcgatgccag gtaaaatagc ttcctggtat ataggtaata atgatcaacc atggataaat
      901 tttttcgttg atacaagctc cgttcatttt tacgttgacg aaccgacctg tcaaaccgtt
      961 gccctggcac aagatcaggg caacgtcagt ggcaaccagg taacgcttgg gaacagctat
     1021 gtatcagaag ttaaaaatgg gcttacgcgg gaaattcctt tttctatccg tgctgaatac
     1081 tgttatgcca gtaaaattac ggttaagttg aaagcggcaa ataagcccag cgacggcaca
     1141 ctggtaggta aaacaacggg ctctgcttcc ggcgtggctg taaaagttaa ttcaacttac
     1201 gacaatagca aagttttgtt gaaagcagat ggtagcaacg cggttgacta taacttcgcc
     1261 atctggtcaa acaacctgct aaatttcccc tttacggcac agttggtacc ggatggtagc
     1321 ggtaatgctg tcggtgttgg aaccttctct ggtaacgcga ccttctcctt tacctacgaa
     1381 taagtaaaac gtcattcctt cctcatcgca ccagagttct gctctggtgc gaatattttc
     1441 cccacactaa aaaccattgc ccccctgatt tcgactgtta gaatgcgatc aatctgactt
     1501 tagccatcag ggtacgttat gaaaccgacc accattgctt cattgcaaaa atgcaaacag
     1561 gaaaaaaaac gtttcgcgac catcaccgct tacgactata gcttcgccaa actctttgcc
     1621 gatgaaggac ttaatgtcat gctggtaggc gactcgctgg gcatgacggt tcaggggcac
     1681 gactccaccc tgccagttac cgttgctgat atcgcctacc acactgccgc cgtacgtcgt
     1741 ggcgcgccaa actgcctgtt gttggctgac ctgccgttta tggcgtatgc cacgccggaa
     1801 caagcttttg aaaacgccgc aacggttatg cgtgccggtg ctaacatggt caaaattgaa
     1861 ggcggtgagt ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt
     1921 ggccacttag gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg
     1981 cgcggcgatg aagcgagcga tcgactgctt agcgatgcat tagccttaga agccgctggt
     2041 gcacagttgc tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgaagca
     2101 ctggtgatcc cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg
     2161 atgcacgatg cgttcggcat taccggcggc catatcccta aatttgccaa aaatttcctc
     2221 gctgaaacgg gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc
     2281 gtttatccgg gcgaagaaca cagtttccat taaggagtga cgttgtgtta attatcgaaa
     2341 ccctgccact gctgcgccag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc
     2401 tggtacctac catgggcaac ctgcatgatg gccacatgaa gctggtcgac gaagccaaag
     2461 cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg
     2521 aggacctggc tcgttaccca cggaccttgc aggaagactg cgaaaaactg aacaagcgca
     2581 aagtggatct ggttttcgcg ccctcggtaa aagagatcta cccgaacggt actgaaaccc
     2641 atacttacgt tgacgttccc gggctttcga ccatgctgga aggcgccagc cgtccggggc
     2701 attttcgcgg cgtgtcgact atcgtcagca agctgtttaa cctggtccag ccggacatcg
     2761 cctgcttcgg tgaaaaggac tttcagcaac tggcgctgat ccgcaaaatg gttgccgata
     2821 tgggcttcga tattgagatt gttggcgtcc cgattatgcg cgccaaagat ggccttgcgc
     2881 tcagttcccg taatggttat ctgacggcag aacaacgcaa aattgcgccc ggtctgtaca
     2941 aagttttaag ttcgattgcc gacaaattac aggctggcga gcgggatctc gatgaaatta
     3001 ttgccattgc agggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc
     3061 gcgatgccga cacattgctg gaagtctctg aaaccagcaa acgggcagta attctggtag
     3121 ccgcctggct tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact
     3181 tatactctaa ataattcaag ttgcaggaag gcggcaagcg agtgacaaat tcgtcaggaa
     3241 cgaatttgcc cagccgcagg ctggccttcg gtgagagaca tggatgtctc tcattaatcc
     3301 ccaggagctt acataagtaa gtgactgggg ttagcgaacg cagacgcagc acatgcaatt
     3361 tgaagtatga cgagtataac tggcgctacg gctgatggcg ccaattattg atttgcccca
     3421 cgtcatggcg atgacgtctg taagcgatta ttcataacgc agacaactta gttaatcagc
     3481 ctatcaatct cagcaagaga taaatttgtc atctctgcaa cgtcttcccg agacatccct
     3541 ttactcagaa aacgctgggc gaattcttgc ctttcctcct gtcttccctg ctgaatcccc
     3601 ttctcaatcc ctttctcttc aaaccactgc gccagcgtca ccatagactc ccctcccgtt
     3661 tccctgtctc tcagcacacc ataaaacaaa tccgcttgtt cagtatgacc gcgttgcaac
     3721 atatagtttc gcatggcaac taactgactt tcgctagtgt acccttcgtc aatcagcgtg
     3781 accagttgct ccagcaataa cattaagtcg cgctggcgaa tatgtttttg cagtagttcg
     3841 agaatcgcaa tccgccgatg ttgcatgatt tcgtcatccg gtgtgatggt gatatccacc
     3901 agcgggaaag gactgttata gacgcgtcgc gccagctccg gcgagtaaaa catatcaaac
     3961 cagcacattg agagcggata aggtgtggcc tcgccctgat aaaacagtat cggcaccacc
     4021 agcggcagct tatcgtggtc agcctccaga tgccggtgca tggcggcaat agaataacgc
     4081 attatgcgaa aagccatttt cttatctagc ttgctttgat gttcaatcac tacatgcaga
     4141 taaccgggat tgccctgcat ttgcacggaa tagagcacgt ccgtgctgtg tcctttcagg
     4201 ctctcttcaa tgaaactccc cgactctaaa tgaagcgtat tgaggtcaca aagttcgcgt
     4261 aattccactg gcaaatgtat ctccagaaag tcgcgagccg tctccgcatg cattaaaaat
     4321 tgtttaaata ccgcgtcatg cggtgtggta ctcggtgcat ccatcttccc tgtcttccgt
     4381 gttgtcttgc gtgaaggcag tatccagtca aggcgtgcta tcgtcagcca cctctttatt
     4441 gtttaccgac tagcgttcag agaaatatca attcgttaca ggcgatacat ggcacgcttc
     4501 ggcgcggtac gcaaactgct gatgcaaatg ccgtcagatg agtagacact aaacaaaaat
     4561 cgggcaatac tgcgtgagaa tttcctcaaa gcaggcatcg cctgcttcgt taacgacagg
     4621 gtagaaaggt agaagttatg attcgcacga tgctgcaggg caaactccac cgcgtgaaag
     4681 tgactcatgc ggacctgcac tatgaaggtt cttgcgccat tgaccaggat tttcttgacg
     4741 cagccggtat tctcgaaaac gaagccattg atatctggaa tgtcaccaac ggcaagcgtt
     4801 tctccactta tgccatcgcg gcagaacgcg gttcgagaat tatttctgtt aacggtgcgg
     4861 cggcccactg cgccagtgtc ggcgatattg tcatcatcgc cagcttcgtt accatgccag
     4921 atgaagaagc tcgcacctgg cgacccaacg tcgcctattt tgaaggcgac aatgaaatga
     4981 aacgtaccgc gaaagcgatt ccggtacagg ttgcttgatt gttatccctg cggctggtta
     5041 ctcaccagcc gcgacatcgt ctccagcgaa tccgttctta agatataaag tcgttttagt
     5101 aacaacggat tatcccccgg ttttactttg cctttcatgg ttgtcaccgc caggtgaaat
     5161 ccggcatcgt ttgctgcctt cacggcgttg tcattaaatc cgccaaacgg atacgaaaga
     5221 taccagacat gcggattaaa ttgcgccaga gcgcggcgtg aacgtgcaaa atcaaacaga
     5281 atattgtgct cactacggct cagtaatatg ggtcggcgat aaccatctac ccgatgcaaa
     5341 aaatgggtat gtgactggaa atcaaataca tcgcgaattt cgttaagctc agaaacgctc
     5401 ataaattgca gcgattttgg gttccacttc tgcgggtgac gtttgatgcg tgaggtaaca
     5461 ataaacgccg tcgccttcat gccatattgt ttcaacacag gatacgcata gcggctcacc
     5521 gacttgaggc catcatcaaa ggtaatcacc accgctcgcg cagggagatt gatcttattc
     5581 ttcacgtagc cttccagctg caccatgctc agtgtcgcgt atcccctgtc acgcagccag
     5641 gccatctggt tattgaaagc gcgtaccgat gtggtcgtcg aagtatggcg aaaacgggtg
     5701 ttttcttcgt cgcgcagaat atggtgatag gttagcaccg acaggccatt atcgggttgg
     5761 gcatccagtg cgctgatata ggccagtcga tcgccaatac ggatctgata ccaggtttga
     5821 tttaacctgt ctttcagttt atgcaaaatc gggtagcgca aattgtccgc cagtacccca
     5881 aatggcgcac ttcccgcact cggcgcgtta tagaccggcg tatctttcca ggtaactaag
     5941 ttctgattac tcagcggctt gttgaggtcg cccaaaccgt cttcaacttt ttgtcgcccc
     6001 tgaaccggct cgagatgacc tttatcgata aaacctttgc caaagccaaa attaaatgcg
     6061 taataacttg cggcagtggg ctccacggca atgatttgtc cggcccgaat attccccacg
     6121 gtcaccatct tgtcaccaat ttgcgcccag accgcagcat tttcgatggt ttgcatataa
     6181 cgggcaggta acgcggcact gacacttgcg gtaaacagca tcagcaggag aataacagct
     6241 tgtttgtaca tgaaaaaata accgagggtg aaacaacgct tttattgtag caaaagcgcc
     6301 atcagaatcg cgggttaata ctcatacaaa ttatgtttga ggacgaaagg cggatttttt
     6361 tgttgttggt gccaaagact actcacctcc ggcgcaccca gttcgacaat acgctcgcga
     6421 aactctgaac tggtcatggt ttcacggcaa gccatcatct gttcaattaa cggtaacgtg
     6481 acaccagaaa tcacttcgca acgggagtgt ttgtggctta ataatgaagc cacgcgatac
     6541 ggtggcgctc cggctatatc ggttaagaag atgacaccct caccagagtc cgcttcatgc
     6601 agagcatcgc acatcatgcg gctgagcata ttagagctta atccgcgcca gaaattcacg
     6661 gcccggcact gaagaagtgc cccatgtttt ttctccagcg catccagtat ctcttgcgcc
     6721 cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag ccgaaaatca
     6781 gtctaccctg accattgttg ataaagggtg atagccgtca aaactcctct cccccaaatc
     6841 cagaggagag gaaatagcct tagctacgca aaccacgtcc acgttggatc agcgaccaac
     6901 agatcaaata aaacgccaca ataaagacca ccagtacgcc aaaggtagtg accagcggaa
     6961 catcattgat accgaggaag ccgtagcgga aaccactgat catataaacg attgggttca
     7021 ggtgcgacag cccttgccag aacggcggca acaaagtcag tgagtaaaag accccgccca
     7081 aatacgtgag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg aacgttttgg
     7141 caaacacacc gttcagcaaa cccgcaaggg agaacaacac cgccgtgagc accagcgtta
     7201 aggcaacgaa tacccacgaa tgcacctgaa atggcacaaa aaacagtgaa attgccgtca
     7261 ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg gcaataatga
     7321 cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaac ttggcaccaa
     7381 aaaatgatga cgcaacgttg gcgtaggcat tggtgatcac cgacatcatg atcagccccg
     7441 gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc aatacgcgaa ccaatcaggt
     7501 taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc gtctgcaccc
     7561 agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc acccagtaaa
     7621 gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca gctcttccag
     7681 acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta actgcgtaaa
     7741 tacgctgttg agcccctgct cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag
     7801 tcgatactga tagccatcga gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa
     7861 ggtttccgat ttcagcttcg ccagcagcgc cttcatcgag gtattttcca ccagctcacc
     7921 gtgttgaata atgccgatat tgcggcacag catttctgct tcttccaggt agtgtgtggt
     7981 gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg
     8041 aagttcaata tccacgcctg cggtcggttc gtcgagaatc agtagtttag gttcatgcat
     8101 taacgcacgg gcaatcatta aacggcgctt catcccgcca gataacatac gcgcacgttc
     8161 gttgcgtttt ccccataaat cgagctgctt aagatacttt tcgctgcgga tgtacgcttc
     8221 tttgcgctcc acaccgtagt accctgcctg attaaccaca atttgctgca cggtttcaaa
     8281 cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt tcacgacatc
     8341 cttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta ccagagagct
     8401 gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg cataaaaatc
     8461 acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg gataggtttt
     8521 tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt acgttcttac
     8581 actttatctg tggtttaaat cgtcccggag ttgccctata ttagccaaac gtaattattt
     8641 ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa caatgcacta
     8701 tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc acaagcgcaa
     8761 aaaccgcgct ttctatggat tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc
     8821 ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt cattcacacc
     8881 gacctgaact gcctttccgt ggttcagtat gcagtggatg tactcgaagt tgaacacatt
     8941 attatctgtg gccactacgg ttgcggcggc gtacaagccg ccgttgaaaa cccggaactg
     9001 gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca tagctcattg
     9061 ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa
     9121 caggtttata acctgggcca ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa
     9181 gttaccattc acggctgggc ctatggcatt cacgacggct tgctgcgtga tctggatgtc
     9241 acagcgacta accgcgaaac ccttgagcaa cgttaccgtc acgggatttc caacctcaag
     9301 ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc cggcaacttc
     9361 acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg taacgctgtg
     9421 cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaacca ataaattcta
     9481 ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc gccagcgact
     9541 tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattcccc gagtcgatga
     9601 tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct ttgaggattt
     9661 tcacatcacg ggtggtggac atgccgctac cgtagctgga ggcggtcata aagtcgactt
     9721 catgagatac ctgaacttca cggcacaggt ccgccataaa cataaatgag ccacgcagca
     9781 gaccaaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca
     9841 gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca gtatgtttca
     9901 tatctctaac catatgattt aaaaacaaat cacttcgcac gggcaggttt tggcaccgca
     9961 acaaagcgca agacacgcag tataccagta aaactattca ttcgcggaat ctgttaataa
    10021 agctcgaatt gtgatgacga tcacacatgt taaaacctat aattaattgc tactaaaata
    10081 ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg cgacgattac
    10141 tcgtcacgct aacagccctt tttgcagcgc tttgcgggct gtatctactc attggcggag
    10201 gctggctggt cgcgattggc ggctcctggt actactctat cgctggcctt gtgatgctcg
    10261 gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac gccgccctgc
    10321 tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg gcgctgactc
    10381 cgcgcagcga tattctggtc ttcttcggca tctggctgat cctgccgttt gtctggcgtc
    10441 gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg ctgattagcg
    10501 gtagtatcct gacctgggcc ggatttaacg atccgcagga aatcaacggc accttaagcg
    10561 ccgatgccac acctgcggaa gctatctcac ccgttgccga tcaggactgg cctgcctatg
    10621 gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc gataacgtcc
    10681 ataatctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg aacgatccgg
    10741 gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac ctgtgtaccg
    10801 ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg cattacgatc
    10861 ctgagctgaa aactaacgag tctttccagc acgtaacctg ccgtggcgtc tcttatcatg
    10921 aagccaaagc agaaacggct tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc
    10981 ttccggtcaa tgatggtcga ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa
    11041 ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccagacacc aaaccgggtc
    11101 tgtatgaacc gacttcgcca ccgattatca ccgataaaac catcgtgatg gcaggttcag
    11161 ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca
    11221 ccggggagct gctgtgggct tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg
    11281 acgaacacac ctttaccttt aactcgccaa actcgtgggc accagcggcc tatgacgcga
    11341 agctggatct ggtgtatctg ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc
    11401 gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc actaccggga
    11461 aactggcgtg gagctaccag accgttcacc acgacctgtg ggatatggat cttccggcac
    11521 agccgacgct ggcggacatc accgttaatg gtcagaaagt gccagttatt tacgctccgg
    11581 cgaaaaccgg caacattttt gtgctcgatc gtcgtaatgg cgaactggtg gttccggcac
    11641 cggaaaaacc ggttccccaa ggtgctgcca aaggcgatta cgttacccct actcaaccat
    11701 tctcggaact gagcttccgt ccgacgaaag atttgagcgg tgcggatatg tggggagcca
    11761 ccatgtttga ccaactggtg tgccgcgtga tgttccacca gatgcgctat gaaggcattt
    11821 tcaccccgcc atctgaacag ggtacgctgg tcttcccggg taacctgggg atgttcgaat
    11881 ggggcgggat ttccgttgat ccaaatcgtg aagtggcgat tgccaaccca atggtactgc
    11941 cgtttgtttc gaaactgatc ccgcgtggtc ctggcaaccc gatggagcag ccgaaagatg
    12001 ccaaaggcac gggtacggaa tccggcattc agccacagta cggtgtaccg tatggtgtca
    12061 cgctcaaccc gttcctctca ccatttggtc tgccatgtaa acagccagca tggggttata
    12121 tctcggcgct ggatctgaaa actaatgaag tggtgtggaa gaaacgtatt ggtacgccgc
    12181 aggacagtat gccgttcccg atgccggttc cggtgccgtt caatatgggt atgccgatgc
    12241 tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg gcagataact
    12301 acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg
    12361 gtggtcaggc tacgccaatg acctatgaag tgaatggtaa gcagtatgtg gtgatctccg
    12421 caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct tatgcgctgc
    12481 cggatgatgt gaagtaagac ttgctcagat tgctgacaac gtgcgcgttg ttcatgccgg
    12541 atgcggcgtg aacgccttat ccagcctaca aaattgtgca aaatcaatgg attgcacagc
    12601 tagcgtaggc ctgataagcg tagcgcatca ggcagatttg cgtttgtcat cagtttaatg
    12661 cccggagaga tccgggcata tttccgaata cggtcttttt ataccgtaaa ccctaacatc
    12721 atccccgtat cttcatgctc cagcagatgg cagtgcgcca tataagcatg ttctttcggt
    12781 gcatcgtgat taaacttcac cagcacttcg ctgacattac cttctacctt aacggtatct
    12841 ttccagcccg cgcgatgagc cgctggcggt ttgccatttt ctgacaagat acggaactgc
    12901 gtgccgtgga tatggaacgg atgcagcatc atgtcgccca cgccagagat aacccaacgt
    12961 tcgtattgcc ctttcgccgc cgcaaacatc ggcttgttca tatcaaacgc ctgtttgttg
    13021 gcatggtgga aatcgaactt cccgccgtgg ttcatatgat tcatattgcc gtgccccata
    13081 tggcccatca tctggctgtg atccatcccg gccatcgcct gatcgccata tttctccatt
    13141 agcatctgca tccccatcat atcgagcatc gggtccatag agagttgcag cttgcgtacc
    13201 gtcagccctt ccagcgaagg taacgcaggc aggctactta atgtgtctgg caaagcaccg
    13261 gaggcactaa tagcaatcgg ctgaatccgc attaccggat gaggcttatc aaacggcgca
    13321 atcgccatcc ccatctggct gaccggcagc gtcaccaggt caaagggttt gttatcgtta
    13381 acctccacca gcacttcaaa acgctcgccc atcagcaccg gcagttcgct caccttcact
    13441 ggttcaggta gcagaccacc gtcgctggca atcacataca gcgggcgatt gtcgctggtg
    13501 gcgaaattga gcgaacgggc attacagcca ttgagcaaac gcaggcgcag ccaaccacgc
    13561 ggggcagcgt gttgcgggta gattgcaccg ttggtcagca acgtatcgcc aaaccagccc
    13621 acggcggcgg tcatcacatc cagttgataa tcaatctgcc cgtcggcgct aaatttctta
    13681 tcctgaacga tcaccggaac atcatcgata ccccactgtt ttggcagcat taatttcagg
    13741 atctcgtcat cttcaatcac caccagccca gccagcccca tcgccacctg tcgcccggtt
    13801 ttgccgtgct gatgcggatg gaaccagcag gtagcggcag gttgatcaac gttcaacgtc
    13861 accgagcgct tgccacctgg cggaataatt ccctgcgggc cgccgtcgac ttcacccggt
    13921 acttccagcc cgtgccagtg caacgttgtc tcttccgtca gttggttgta gatatcaacc
    13981 gttaccgctt tgccgcgctg taatttcacc gccggcccca gcagattgcc gttatagccc
    14041 caggtagttg cagttttccc gccaaaggtg gactggcctg cgccaatagt taactgaatg
    14101 cgattacggg catcggtcgt gagcaaatca gggatcggta acgttgggcg ttctgccgca
    14161 aatactgcgc ggctccacag cggcaaagcc gaagccacac ccagcgcgac ggaatatttt
    14221 aagaaatcac gacgttgcat agttatttcc ttattcttaa gcaggcgaaa caaaatcaaa
    14281 cgttgagcat agtccttccc cttacgggaa ggtcaagccg cagacacaat ttaatcgaca
    14341 ataatggtcg tcgcctcgct cgcagtgtgc taacgtttat cttctttaag cccctggtag
    14401 aagcaatgaa gacgtttttc agaacagtgt tattcggcag cctgatggcc gtctgcgcaa
    14461 acagttacgc gctcagcgag tctgaagccg aagatatggc cgatttaacg gcagtttttg
    14521 tctttctgaa gaacgattgt ggttaccaga acttacctaa cgggcaaatt cgtcgcgcac
    14581 tggtcttttt cgctcagcaa aaccagtggg acctcagtaa ttacgacacc ttcgacatga
    14641 aagccctcgg tgaagacagc taccgcgatc tcagcggcat tggcattccc gtcgctaaaa
    14701 aatgcaaagc cctggcccgc gattccttaa gcctgcttgc ctacgtcaaa taatccctga
    14761 tacctttttg tagaaatatt gaccgtgcat ccgcggtcaa tgttagctat tatgttgcgc
    14821 ccttttttta cgggtgttaa caaaggaggt atcaacccat ggccgaaaaa aaacagtggc
    14881 atgaaacgct acacgaccag tttgggcagt actttgcggt agataacgtt ctgtatcatg
    14941 aaaagaccga tcaccaggat ctgatcattt ttgagaacgc tgcatttggt cgcgtaatgg
    15001 cgctggatgg cgtagtacaa accaccgagc gcgacgagtt tatctatcat gagatgatga
    15061 cccatgttcc gctactggcc catggtcacg cgaaacatgt gctgattatc ggcggcggcg
    15121 acggtgccat gctgcgtgaa gtaacccgac ataaaaacgt tgagtcaatc acgatggtgg
    15181 aaatcgatgc gggtgtcgta tcgttctgcc gtcagtatct acccaaccat aacgccggta
    15241 gctacgacga tccgcgcttt aagctggtga tcgacgatgg cgtcaatttc gttaatcaaa
    15301 ccagccagac ctttgatgtc attatctccg actgcaccga tcctatcggt cccggcgaaa
    15361 gccttttcac ttcggcattt tatgaaggct gcaaacgttg cctgaatcct ggcggtatct
    15421 tcgtcgcaca aaacggcgtc tgctttttac agcaggaaga agccatcgac agccatcgca
    15481 aactcagcca ttacttcagc gacgttggct tttatcaggc ggcgatcccg acctattacg
    15541 gcggtatcat gacttttgca tgggcgacag ataacgacgc cttacgccat ctctcaaccg
    15601 aaattattca ggcgcgtttt ctcgcctctg gcctgaaatg ccgttattac aatccggcaa
    15661 tccatacggc agcttttgcc ttacctcagt atctgcaaga cgcactggct tcacagccgt
    15721 cctaaggaga agataagaaa ttgaaaaaac tgaaactgca tggctttaat aatctgacca
    15781 aaagtctgag tttttgtatt tacgatatct gctacgccaa aactgccgaa gagcgcgacg
    15841 gttatattgc ttatatcgat gaactctata atgccaaccg tctgaccgaa atcctgtcag
    15901 aaacctgttc cattatcggg gctaatattc ttaacatcgc ccgccaggat tacgaaccac
    15961 agggtgccag cgtcactatt ctggtgagtg aagaaccggt tgacccgaaa ctcatcgaca
    16021 aaacagaaca ccccggccca ctgccagaaa cggtcgttgc ccatcttgat aaaagtcata
    16081 tttgcgtaca tacctacccg gaaagtcatc ctgaaggcgg tttatgtacc ttccgcgccg
    16141 atattgaagt ctctacctgc ggcgtgattt ctccgctgaa ggcgctgaat tacctgatcc
    16201 accagcttga gtccgatatc gtaaccattg attatcgcgt gcgcggtttt acccgcgaca
    16261 ttaacggtat gaagcacttt atcgaccatg agattaattc gattcagaac tttatgtctg
    16321 acgatatgaa ggcgctgtat gacatggtgg atgtgaacgt ctatcaggaa aatatcttcc
    16381 ataccaagat gttgcttaaa gagttcgacc ttaagcacta catgttccac accaaaccgg
    16441 aagacttaac cgacagcgag cgccaggaaa ttaccgctgc gctgtggaaa gaaatgcgcg
    16501 agatttatta cgggcgcaat atgccagctg tttaacggct ctggcggagc tcccaggctc
    16561 cgccagattt atttacttct gctgcacgaa attgcggtaa gccgccacga cctgcagaaa
    16621 atcctcaacg ccgcatagcg acaggctttc ttcgtcgtag tagttcatcc cctcttccat
    16681 ttcatcgcca gcgaattcca gttgattggc gcgaaccatc acctcttcac cgtccatcca
    16741 cagggtgtat tcatgccctg cccgttgcca ggaccgttcg ctacctttca gtgcgtgcgc
    16801 tgcttgttcc acttcatcaa gcaaggccag gttttctttc acctcttcat taaaccagtg
    16861 cccgaccact tcatgcccca tggacatacg cacctttacc actccggtaa tatcgcgcag
    16921 aaattcgtaa tccatagtgt gttcctctgc tcccggcaat gcaccgaaat cgcattgcgc
    16981 cattgatgta attatcgcag ccttaccgga gaaaaacagc ggaacggcga tggtttagaa
    17041 ataaaaaaat gcccgggaat ctcattcacc gggcattgtg tcgtttatgc gcagcgcgtg
    17101 cgctgacttt ttaaaccgca gtctggaaaa tcaccccatc ggctttctcg gtgtactgag
    17161 aaagctggtt gaagttcaga taacggtaag tatcaacggc tgttttatct acctgcgcca
    17221 cgtaggtctg gtactcttcc ggcgtcggca gtttgccaat cagcgccgca acagccgcca
    17281 gttccgcaga agccaggaag acattcgcgc cagtacccag acggttcggg aagttacggg
    17341 tagaggtgga aaccaccgtt gcaccgtccg ccacacgcgc ctggttaccc atacacaggg
    17401 aacagccagg gatctcgata cgcgcaccac tcttaccgaa gacgctgtag tagccttctt
    17461 cggtcaactg tgcggcgtcc atacgggttg gcggtgccac ccacaggcgg gtcggcaact
    17521 gacctttatg cgcatccagc agtttacccg cagcacggaa gtgaccgatg ttggtcatgc
    17581 aggaaccgat aaacacttcg tcgatcttct caccctgtac cgcagacagc ggacgcgcgt
    17641 catccgggtc gttcggagca cacaggattg gctctttaat atccgccaga tcgatgtcga
    17701 tcactgccgc gtattccgca tctgcatcgg cttccagcag ctcaggattc gccagccatt
    17761 tttccatgcc ctgaatacgg cgttccaggg tacgacgatc gccgtaacct tccgcgatca
    17821 tccacttcag caggacgatg ttggagttca ggtattcgat gatcggttct ttgttcagct
    17881 tgatagtaca accagcggca gaacgctcgg cggacgcatc ggtcagctca aaggcctgct
    17941 caactttcag atccggcaga ccttcgattt ccaggatgcg gccagagaag atgtttttct
    18001 tgcctttctt ctcaacggtc agcagacctt gtttgatcgc atacagcggg atcgcgtgta
    18061 ccagatcgcg cagggtgatg cccggctgca ttttgccttt gaagcgcacc agaacggatt
    18121 ccggcatatc cagcggcatt acgccagttg cggcagcaaa cgccaccaga ccagaacccg
    18181 ccgggaaaga gataccgatc gggaaacggg tatgggagtc accaccggta ccgacggtat
    18241 ccggcagcag catacggttc agccaggagt gaatgacgcc gtcacccgga cgcagcgaca
    18301 caccgccacg gttcataatg aagtccggca gcgtgtggtg cgtgttcacg tcaactggct
    18361 tcggatacgc cgcggtgtgg cagaaagact gcatcaccag gtcagccgag aagcccaggc
    18421 acgccaggtc tttcagttca tcacgggtca tcgggccggt ggtgtcctga gagcctacag
    18481 aagtcatttt cggttcgcag tacgcgcccg gacgaatgcc tttcacgcca caggcacggc
    18541 ctaccatttt ttgcgccagc gagaagccgc gatcgctctc agcgacatct ttcgcctgac
    18601 ggaacacatc actgtgcggc agaccaagtg cttcacgcgc tttggtggtc aggccacgcc
    18661 caataatcag cggaatacgg ccgccagcac gcacttcatc aatcagcacg tcggttttca
    18721 gttcgaaggt cgccagcagt tcgccggttt cgtggttacg cacttcacct ttgtacgggt
    18781 aaacgtcaat tacgtcgccc atgttcaggt tagagacgtc gacttcgatt ggcagtgcac
    18841 ctgcgtcttc cattgtgtta aagaagattg gtgcaatttt accgccgagg cacaaaccac
    18901 cgccgcgttt gttcggcaca tgtggaatat catcacccat aaaccacagt acggagttcg
    18961 tggcggattt acgcgatgaa cccgtaccca caacgtcacc gacgtacgcc agcgggaaac
    19021 ctttctgttg cagagcttca atctgtttga tcgggccaac aacacctggc tggtctggct
    19081 cgatgccttc acgggcgttt ttcagcatcg ccagcgcgtg cagtgggata tccgggcgtg
    19141 accacgcatc cggtgccgga gagaggtcat cggtgttggt ttcgccagtg actttgaaga
    19201 cggtaacggt cagtttttca gccagcgccg ggcgattcag gaaccattcg gcatccgccc
    19261 aggactgcat tacctgcttc gcatattcgt tgcctgcttt cgctttctct tctacgtcat
    19321 agaagttatc gaacatcagc agtgtgtgag aaagtgcttt ggcagcgatc ggtgccagtt
    19381 tggcatcatc cagcgcgtcg atcagcggat gaatgttgta accaccctgc atggtgccca
    19441 gcagttcgat ggctttttcc ggagtcagca gaggggattt ggcttcgcct ttagcgatag
    19501 cagccaggaa gcctgctttg acataggcgg cttcatcgac gcctggggga acacggttgg
    19561 ttaacagatc taacaggaat tcttcttcgc ccgcgggcgg gtttttcagc agctctacaa
    19621 gtgcggccat ttggtttgca tccaggggtt tgggcgcaat cccctcagcg gcacgctcag
    19681 ctacgtgctt acggtattct tctagcacga cggttctcct cgctctcatt gtcatagtgc
    19741 ggcaggcgat tctcttcacg ctcctgtgag acagcagttt gtagggtaaa tgcccagtac
    19801 cgcaacgggc agaatagcag gattttgacg aggtgttaat ctgtttacaa aaaagcaaca
    19861 taaaaaagtg gctgaatcgt taaggatggt ctagagattg tttccctccg taaaaacttc
    19921 gttaattcgc atggtgataa tcacctttct caacgaatcc caagattagt caaaatttaa
    19981 actaccgcct ctttatactc ggattcacag cacctgcggg tggcagttcg ccgaccattg
    20041 cgatttcctt gagatccgaa ttatgaaaat gactttgccg tttaaacccc atgtgctggc
    20101 gctaatttgc agtgccgggc tttgtgccgc ctctaccggg ctatatataa aaagccgcac
    20161 agtggaagcg cctgtggaac cgcaatcgac acaactggct gtgtctgacg ctgccgcggt
    20221 tacgtttcct gcaacggttt ccgcacctcc cgtaacaccc gccgtcgtca aatccgcatt
    20281 cagcactgca caaatagatc aatgggtcgc gcccgtcgcg ctgtatcccg acgccctact
    20341 ttcgcaggtg ctgatggcat caacctatcc gacaaacgtt gctcaagcag tgcaatggtc
    20401 gcacgataat ccacttaaac aaggcgatgc tgctattcag gcggtatctg accagccgtg
    20461 ggacgccagc gttaaatcac tggtggcctt tccacaattg atggcattga tgggcgaaaa
    20521 cccgcaatgg gtgcaaaacc tgggcgatgc ttttctggcc cagtcgcagg acgtgatgga
    20581 ctcggtacag cgattgcggc aactggcgca acaaaccggc tcgctgaagt catcaaccga
    20641 acagaaagtt attaccacaa cgaagaaagc tgtaccggta aaacagacag tcacggcacc
    20701 cgtcatacca tccaatacag ttttaactgc caaccccgtc attacagagc cggcaacaac
    20761 cgtcatttcc attgagcccg ccaatcctga tgtggtctat attcccaact acaacccaac
    20821 cgtggtttac gggaactggg ccaatactgc gtatccgccg gtttatctgc caccaccagc
    20881 cggagaaccg tttgttgaca gctttgtacg cggattcggc tatagcatgg gcgttgctac
    20941 cacgtacgca ctattcagca gcatcgactg ggacgacgac gatcatgacc atcatcatca
    21001 tgacgatgat gattatcatc accacgatgg cggtcatcgt gacggtaatg gctggcaaca
    21061 caacggcgac aacatcaata tcgacgtcaa caatttcaac cgtatcaccg gtgagcatct
    21121 tactgataag aatatggcat ggcggcacaa tccaaactac cgtaatggtg tgccctatca
    21181 tgatcaggat atggcaaagc ggtttcatca aaccgatgtc aacggcggaa tgagcgccac
    21241 gcaattacct gccccaacgc gcgacagcca gcgtcaggcg gcagcaaatc agtttcagca
    21301 acgaacacac gccgcaccag tcattacacg agatacccaa cgtcaggcag cggcacagcg
    21361 gtttaatgaa gctgaacact atgggagcta tgacgacttc cacgacttca gccgtcgcca
    21421 accactgacc cagcaacaaa aggacgccgc tcgtcagcgt tatcagtccg cctcgcctga
    21481 gcagcgccag gcagttcgcg agagaatgca gactaacccg aagatccagc agcgaagaga
    21541 ggcagcgcgt gagcgcattc agtccgcctc gcctgagcag cgccaggcgg ttcgcgagaa
    21601 aatgcagact aacccgcaga accagcagcg aagagacgca gcgcgtgagc gtattcagtc
    21661 cgcatcacca gagcagcgcc aggtgtttaa ggaaaaagta cagcagcgcc cactgaatca
    21721 acagcaacgt gataacgccc gccagcgtgt tcaatcggca tcacctgaac aacgtcaggt
    21781 ttttcgggag aaagttcagg agagccgccc acaacgtcta aacgacagta accatactgt
    21841 caggctgaat aacgagcaac ggtcagcagt acgcgaacgt ctctctgaac gtggagcaag
    21901 aagactggaa aggtaaattg cagacgtaaa aaaagcggcg tggttagccg cttttttaat
    21961 tgccggatgt tccggcaaac gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg
    22021 gtaatgctac cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc
    22081 gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca tttcgattgc caggccgatt
    22141 tcacccagca gctcgccgcc gttagtaccg acaatcgcac caccgatcac acggtgagat
    22201 tctttgtcga aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca
    22261 gaagcagccc acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc
    22321 tcagtcagac ccacccatgc aacttctggt tcggtatagg cgatggacgg gataactttc
    22381 ggatcgaagt agtgtttctt accggcgata acttcagcgg caacgtgacc ttcgtgaaca
    22441 cctttgtgtg ccagcatcgg ttgaccgacg atatcgccga tagcaaagat gtgcggtacg
    22501 ttggtacgca gctgtttgtc aacgcggatg aaaccacggt cgtcaacttc cacgcctgct
    22561 ttgcctgcgt cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg
    22621 taacgctgcg gttcagcggg tgcttttttg ccttccatcg tcacataaat gccgtcttct
    22681 ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc
    22741 ttggtgaaga ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca
    22801 accacgtcaa tctgtgaacc cagcgcgtgg tatacggtac ccatttccag accgatgata
    22861 ccgccgccca ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag
    22921 tcccagatac gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgcc
    22981 gcgatgatcg cgttgtcgaa gttgatcacg gttttaccgt tctcaccttc aacttccagg
    23041 gtgttagccc cggtaaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc
    23101 ataccagcca gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg
    23161 tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct
    23221 tcgataactt ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa
    23281 acaccgccaa gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa
    23341 cggaaggcag cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt
    23401 tcagtactca tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag
    23461 gccgcagcgg cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg
    23521 gattctggcg cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc
    23581 ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa
    23641 acaacaaacg gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac
    23701 cagaaaaaag ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca
    23761 gacagcgtgt tgttaatgat ggtaatgaaa cgagcaccat cagcaccgtc gatcacgcgg
    23821 tggtcgaagg agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac
    23881 accggctcca tcgcggacct ggaaacgccg aggatagcca cttccggcgc gttcacaatc
    23941 ggcgcgaagt gggtagtacc caggccgccg atgctggaga tggtgaagca accgccctgc
    24001 atttcgcccg cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga
    24061 gacagctcga tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc
    24121 ggggtatcca ccgccacacc gatgttgatg tacttcttca gggtcagacg ctgaccgtct
    24181 tccgacagcg aactgttgaa gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg
    24241 atgaagacaa ccggggttat cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc
    24301 tgtttacgga acgcttccag ctcggtgata tcggttttgt cgaagtgagt aacatgcggg
    24361 atcatcaccc agttacggct caggttcgcc ccagagattt tctggatacg gcccagttcc
    24421 acttcttcga tttcaccaaa cttgctgaag tccaccttcg gccacggcag catgcctggg
    24481 ataccgccgc cagtcgccgc cggagctgct tctgcacgtt tgatagcttc tttcacgtaa
    24541 gcctgaacgt cttcgcgcag gatacgacct ttacggccag tgcccttcac tttcgccagg
    24601 ttaacgccaa actcgcgtgc cagacggcgg atcagcggag tcgcgtgaac gtaagcgtcg
    24661 ttttcagcaa attcagattt gccttccgct tttgcagccg gagctgctgc cggggcttca
    24721 gcttttgctg ccggagccgg cgctgccgct tcctgtttcg caggagctgc agcaggcgct
    24781 gcgccttcaa cttcgaagat cataatcagc gaaccagttt tcactttatc gccaacgttg
    24841 actttcagtt ccttcacgac gcctgcaaac ggagccggaa cttccataga cgctttatcg
    24901 ccttctacgg tgatcagtga ctgttcagcg gcaactttgt cgcccacttt caccatcact
    24961 tcggtgactt caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagct
    25021 gctggtgcag gagccgctgc cggagccgct tcctgtttag ccgccggagc tgccgcgcct
    25081 gcttcacccg cgacttcgaa gaccataatc agcgagccgg tagacacttt gtcacccacg
    25141 ttcactttga tctctttcac ggtgccagca aacggagccg gaacttccat agaagccttg
    25201 tcgccttcta cggtgatcag cgactgttca gcttcaactt tatcgccaac tttcaccagg
    25261 atttcggtca cttcaacttc gtcgctgccg atatccggaa cgttaacgtc ttttgccgct
    25321 gcagccgctg gtgctgctgc cggagctgct tctttcttct cttctgcctg agcaggtgca
    25381 gcgtctgctg caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgggtttta
    25441 tcgccaacag agactttgat ctctttaaca atacccgcct gcggagacgg aacttccata
    25501 gaggctttgt cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact
    25561 ttgaccagga tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg
    25621 atagccatta ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt
    25681 tgaatttggc gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt
    25741 cgcccagcgc cgctacaacc acgtaggaag catcaacttc gaagtggtga cgcaggttct
    25801 cacggctgtc ggaacgaccg aagccatcag tacccagtac gcggtagtcg tcagccggta
    25861 cgtaagtacg gacctgctca gcgaacagtt tcatatagtc ggtagatgcc actgccggag
    25921 cgtcgttcat cacctgagcg atatacggaa cgcgcggagt ttccagcggg tgcagcatgt
    25981 tccagcgttc acaatcctga ccatcacgcg ccagctcggt gaaggaggtc acgctataaa
    26041 cgtcagaacc tacgccgtaa tctttcgcca ggatctctgc cgcttcacgg acgtgacgca
    26101 ggatagaacc ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt
    26161 tgtagatacc tttacggata ccttcctcag caccttccgg cattgccggc atgtgatagt
    26221 tttcgttcag cgtggtgatg tagtagtaaa cgttctcttg tttttcaccg tacatacgct
    26281 ccagaccgtc atgcatgatg acagcaactt cgtaagcgta agccgggtcg taagagatac
    26341 agttcgggat agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcgc
    26401 cgttcagggt ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtcgc
    26461 cagccgccca gcacagatcg ccaatacgct ggaagccgaa catcgagtaa tagatgtaga
    26521 acggaatcat cggcagattg ttggtgctgt aagaggtcgc cgctgccagc caggaacaac
    26581 ctgcgcccag ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt
    26641 aagcaacctg ctcgcggtcc tgcggggtgt actgctgacc gttcgggctg taaataccaa
    26701 tctgacggaa cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca
    26761 gacgatcttt gatcgacttg ttcttcagca tcacgttcag agcacgaacg aaagcgatag
    26821 tggtagagat ctctttgctc tgctcttcca acagcgcgcc gaagtcttgc aggctcggca
    26881 gctcaagctt ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac
    26941 gctgagcgtg cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcagtt
    27001 tttcgatatc tgcatcagac accggcacat tgaaacggtc gcggatatga cgcacaccgt
    27061 ccatgttcat tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc
    27121 cgtaaccttt aatggtatga gcaaggatta ctgtcgcttt gcctttggtt tcctgcgctt
    27181 tcttgaatgc agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct
    27241 gctcgtcagt ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt
    27301 cacgaacgta cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt
    27361 tcatcagctg gatcagttta ccgctggtat ccttacgcag cagttcatcc caacggctac
    27421 cccacatcac tttgatcacg ttccagccag caccttcgaa gatgccttcc agttcgttga
    27481 tgatcttgcc gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga
    27541 agaccaggtt atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt
    27601 cgtccatttc accgtcaccg aggaacgcgt aaacggtttg tttagaggta tctttcaggc
    27661 cacggtgttc cagatatttc aggaatttag cctggtaaat agcaccaatc ggacccagac
    27721 ccatagatac ggtcgggaac tgccagaatt ccggcatcag tttcgggtgc ggataggaag
    27781 agaggccatt gccgtgaact tcctgacgga agttatccag ctgctcctga gtcagacgac
    27841 cttccaggaa agcacgagcg tacacgcccg gggagatgtg gccctggaag taaaccaggt
    27901 cgccgccatc ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg
    27961 ttgcggaaga ctggaaggac gccatatggc cgcccagttc gaggtctttt ttcgacgcac
    28021 gcagcaccgt catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca
    28081 gattacccgg atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac
    28141 ctgtgcctgc ggctacgttt acaccgcctt tgcgggcttc agcaagcagt tggtcgatca
    28201 gatactgagc acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt
    28261 cgcgagtttc gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat
    28321 ctatctaata acgttgagtt ttctggaacc tgtcccattg aactctcgcc ggaaagctca
    28381 ataagacagg ttctacgttt agttgccgcg cttttatatg cgcttgattt acaacatctt
    28441 ctggataatt tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgcagagaa
    28501 cgctcacggc ggctctcttc acgacttctg tcgagcaaaa tttcttcgat aaaggccaga
    28561 tggcgatgcg atgcttcgcg cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg
    28621 cgggtgcggt gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag
    28681 ttctggcgga cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc
    28741 acattgtggg ccgcttcggt gacggcaatc tgatactgga gtacggcgtt tgattccgcg
    28801 tccagatcgc cagactgctg cgccagctct atggcgtggt ggagttcacg gatgcgttcc
    28861 ttgtcttcat cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt
    28921 cgtgtttcga gcaagtcata ctgtgattca ggatggtcgg agagcagctc caccagcgga
    28981 tcgctgaagc tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc
    29041 aacaagccct tcgcttcgag acgttgaatc gcctcacgca aggagggacg ggagacgtcg
    29101 aactgttttg ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg
    29161 aggatcaaaa actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg
    29221 ctgtaggcca tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt
    29281 catgtctgtg acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga
    29341 aatcatgaaa ctgtgcacat tttaacaact tgacatatat aacgtttcaa agttg
//
LOCUS       DAZFZC010000062        29242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFZC010000062 DAZFZC010000000
VERSION     DAZFZC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.062"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..260
                     /locus_tag="ACHKXP_003057"
     CDS             78..260
                     /locus_tag="ACHKXP_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO7648015.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(361..690)
                     /gene="tmaR"
                     /locus_tag="ACHKXP_003058"
     CDS             complement(361..690)
                     /gene="tmaR"
                     /locus_tag="ACHKXP_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO7648016.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(862..1920)
                     /gene="yeeA"
                     /locus_tag="ACHKXP_003059"
     CDS             complement(862..1920)
                     /gene="yeeA"
                     /locus_tag="ACHKXP_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7648017.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEINTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(2118..2591)
                     /gene="sbmC"
                     /locus_tag="ACHKXP_003060"
     CDS             complement(2118..2591)
                     /gene="sbmC"
                     /locus_tag="ACHKXP_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO7648018.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(2710..3876)
                     /gene="dacD"
                     /locus_tag="ACHKXP_003061"
     CDS             complement(2710..3876)
                     /gene="dacD"
                     /locus_tag="ACHKXP_003061"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO7648019.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTSQILTAVNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            complement(4698..6797)
                     /locus_tag="ACHKXP_003062"
     CDS             complement(4698..6797)
                     /locus_tag="ACHKXP_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO7648020.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NRIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFEKRLSGVDGFKLIGQVDSFDELRTIVPEVGGQRILLASYYSGGRTG
                     GGEFIARSTVGMSNIPSDDGGIIAVVSSMWYWERVNKENVTVDDFGAVSFEVQDDGSL
                     PSGATSSSAAFQNMFDSLGKIKTPDEKGRGYLIDQPVLLQATDYWSVNLGESTIVKTS
                     TTTTGLDAYIPTQGAYVEGDVNCVFLIGGNDSCRYWTFSGGYIDCHLAPSDNRPVGIY
                     MPQVVGYSIEKVRTRGCKHGLWVKSGWLGGLTLCRFSENTSHGVFYDAARFTSTGGTP
                     AAAVNQAATSLHISSCYVAGAELDGWHMERCQYINFQSTACDNAGSVSGGSSYYFSEC
                     DVAGDIGAESPSPSANAYINMVAGSATLTFNCYDTIDTTNAVILASGGASAVLNLSLR
                     TQRQRLFTITGANTHIIVPRITFWNGATFNFPASLVDTGSTLEIDTGRVGRGVVNYAE
                     SVQAFHKESAYRGQLLSTGSYETFRVSQHKKGFSGPGKTFQIPLTEINGAFPSFLGAA
                     SVYREIFKIRVNNYQSYADGVTFSYDGSTLSVNTANQVVYGGSGISITSISVSGGNLE
                     VTLGASQTNVTFTITPL"
     gene            complement(6902..7627)
                     /locus_tag="ACHKXP_003063"
     CDS             complement(6902..7627)
                     /locus_tag="ACHKXP_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648021.1"
                     /translation="MTFKNVANIGSVVTDKTIDSQSLLMMVNEARKLCGEPSVRNNKF
                     IEKIEDELEGETYTKSVGRKNGADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQ
                     EAHIKSGKSASGLVEYRQARTLKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGF
                     NAIPLPAIEEHYYSAGEVAEQLGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQV
                     EAFRYNLKGVEVLRNLINVSTAA"
     gene            complement(7716..8342)
                     /locus_tag="ACHKXP_003064"
     CDS             complement(7716..8342)
                     /locus_tag="ACHKXP_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO7648022.1"
                     /translation="MNIVAKSDYNFHGVELVPTRDMHGVWFTSSNIASALKYANSRAV
                     TMIYNKYSDEFSAGMTQVLEVSTSGNYRKKVRVFSLRGAHLIAMFARTPVAKEFRRWV
                     LDILDRQAECSPIAKQFTDEELVNLCYLQLWMEKSQQMCKHIYPGMKQIGSELSGRIY
                     DIAYETRYMSEETKKSLLREMKNLDTNNFVVNNAQPMLAKLRGEEWIH"
     gene            complement(8416..8589)
                     /locus_tag="ACHKXP_003065"
     CDS             complement(8416..8589)
                     /locus_tag="ACHKXP_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004136225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO7648023.1"
                     /translation="MSDVLYSGRKSQSFQLRLPARMKEEIRRVAEMDGISINSAIVQR
                     LAKSLREERANAQ"
     gene            8713..8856
                     /locus_tag="ACHKXP_003066"
                     /pseudo
     CDS             8713..8856
                     /locus_tag="ACHKXP_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
     gene            9011..9334
                     /locus_tag="ACHKXP_003067"
     CDS             9011..9334
                     /locus_tag="ACHKXP_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648024.1"
                     /translation="MYTTVIVIAIALIVVQYQLASLKQKISDLKTENEALKNSIKDEK
                     NKLLFTISDIEQSIEIIENNINRLKKEDIHEINDNIKDLKSWLRNVGQIATSTRDKLN
                     PSMDN"
     gene            complement(9335..11494)
                     /locus_tag="ACHKXP_003068"
     CDS             complement(9335..11494)
                     /locus_tag="ACHKXP_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO7648025.1"
                     /translation="MAKAWKDVIASPQYQALTEEQKAQAQAQYFDEVVAPKAGDKWAE
                     ARDQFYAAYPPPQQQKEEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGA
                     SLINAISQGLGGPKVLDDVYRPVDRPTDPYAQAGETIGGYLLPIGTAAKAAGAPAKLA
                     GDIGSAGNMIAGSLADAANQEGDFAQNAAINGGINIGAQGVLSGVGRVIAPRVSQALG
                     GAALNSANDVSRMAKSGAGRQSIASQAANVSEDVAKAAESAGIDINALTPGMRSGSRG
                     IAQAEGALASTPGIVQDAHQAAFNEISSKLSRNLDEFGAASGTASEKSAAIKQRILQN
                     LDQMKDAERAAWDDVRSTMPNQKARMLNGNAVIQAERSAGIPLTPEMKQFVQANNQGG
                     VTFDGMKAWRAKFADAEQKYKRSGEANAARRAGEIRRAITDDMRTMAENGGFLDDWQK
                     ANDLSKARLSAQESAESVFGRDLATDALITNGVKSLQSSSAKGLNGPAGFHSMIRALP
                     ESERVPAISSMLQDAISHGVRGGKADAAGINHIAEILTPQNVKAISRYSSELGRIADA
                     YGTLARAAVKPQQYIERTGRTANVLRDLDAGLSNVTSTVLNAIANSTSGAIVGGAGGG
                     IAGAAAGALVGAGLKGAVSKIATTRSGRYAIEKAVQEATKAVRAGGSKEALAAAERRF
                     MANKAAIKAIRDAVGNEEFNRLARAGIVASLSGMAQE"
     gene            complement(11494..12843)
                     /locus_tag="ACHKXP_003069"
     CDS             complement(11494..12843)
                     /locus_tag="ACHKXP_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO7648026.1"
                     /translation="MATWQQGINSGGFLAGIGTQNENAPKASDINATLGLIRENNELA
                     RSGANNVGLTALRGLAGAADIYKQEQQQKAISAFNKVHADAWASGDPSGLFKFAQENP
                     AFVAQAQQAFSGLNEQQRNDMGDLAMRANVALSQGPEAYSKFITDNKDRLNRVGANAD
                     WMIQTGIQNPEQLSHMLTTMSLGALGPEKAFAVQDKMVGRQQEQQRINETIRNNDMTN
                     ARAIRGQDLSYKAQMARLNHDKYVFKQSQAALERAGQLQDMDVLSLNSQIAATGIDPL
                     TGKAATSARMSQAKRWLDGNNNYNNALITGERGIEKIDSLLGKKELEGIGRFEGRNID
                     GFTSAEGLANRNAIEELKSGAFVQNVQTMRGMGSLSNAEGQKLENLIAKLDITQPEEV
                     VRKQLSEIRSQYSVFQKVAAREAESMGYSSSGYDTYVSERKSGSDSNKSGFSSLWGD"
     gene            complement(12853..13545)
                     /locus_tag="ACHKXP_003070"
     CDS             complement(12853..13545)
                     /locus_tag="ACHKXP_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO7648027.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNKQFNTIMNNLKPFTPLADKYLGSLENLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQQWLSGQMNNYNNLANIGLGALQGQANAG
                     QTYANNMSQISQQSAALAAANANRPSALQQGVSGAASGALLGGGIASALELSTPWGAG
                     IGAGLGLLGSLF"
     gene            complement(13548..14003)
                     /locus_tag="ACHKXP_003071"
     CDS             complement(13548..14003)
                     /locus_tag="ACHKXP_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO7648028.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPQTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQIYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(14003..14704)
                     /locus_tag="ACHKXP_003072"
     CDS             complement(14003..14704)
                     /locus_tag="ACHKXP_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7648029.1"
                     /translation="MADSSLNNPVAVQATRLDASILPRNIFSQSYLLYVINQGADVGA
                     IAGKANQAGQGAYDAQVKNDEQDVELADHDARITANTKAINLLEVRLTTAEGKIVVLR
                     SDVDYLLDEVIDIQAHLVTVDQRLDGVESDVSDIKSDYVSKTVTESQSLASPLDVKTS
                     YSVDGIQVVGARNTGWTAATGTPLLGSFNANQSYTVGTTYTQSEVAALATGLQQARQR
                     ILALETALRLHGLID"
     gene            complement(14704..16122)
                     /locus_tag="ACHKXP_003073"
     CDS             complement(14704..16122)
                     /locus_tag="ACHKXP_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO7648030.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(16123..16623)
                     /locus_tag="ACHKXP_003074"
     CDS             complement(16123..16623)
                     /locus_tag="ACHKXP_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO7648031.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDVSPGINVGYIFADADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAP
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGGE"
     gene            complement(16601..16690)
                     /locus_tag="ACHKXP_003075"
                     /pseudo
     CDS             complement(16601..16690)
                     /locus_tag="ACHKXP_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17238..18533)
                     /locus_tag="ACHKXP_003076"
     CDS             complement(17238..18533)
                     /locus_tag="ACHKXP_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO7648032.1"
                     /translation="MALNEGQLVTYALDEIIETVQNLTPMASKVTKYTPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLSGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     TGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            complement(18533..19444)
                     /locus_tag="ACHKXP_003077"
     CDS             complement(18533..19444)
                     /locus_tag="ACHKXP_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFO7648033.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDNAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKFDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYDAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            complement(19458..21623)
                     /locus_tag="ACHKXP_003078"
     CDS             complement(19458..21623)
                     /locus_tag="ACHKXP_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO7648034.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPVVRKLVAEMRRNPIDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDSDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEKKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQEANSQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLKLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            complement(21624..23123)
                     /locus_tag="ACHKXP_003079"
     CDS             complement(21624..23123)
                     /locus_tag="ACHKXP_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO7648035.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENDEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKPWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            complement(23101..23589)
                     /locus_tag="ACHKXP_003080"
     CDS             complement(23101..23589)
                     /locus_tag="ACHKXP_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7648036.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWVEANPL
                     WEMKAFSYQGEVIQEPIAKMRAMTITGLTLFIDVTLETWRTYRLREDLSEVVTRAEQV
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            complement(23625..23867)
                     /locus_tag="ACHKXP_003081"
     CDS             complement(23625..23867)
                     /locus_tag="ACHKXP_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO7648037.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            complement(24115..24600)
                     /locus_tag="ACHKXP_003082"
     CDS             complement(24115..24600)
                     /locus_tag="ACHKXP_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO7648038.1"
                     /translation="MNNFVEITSRIGRMYQDFLISGKGSGDIIEEIDKLSAELRRNGC
                     VNSIFFETLLKQGFMFDMINYNKVAPSASQKSYVYVLHAEDSGLTKIGFSRRVNKRIS
                     EISRMSGGKLNLIAKIPADRELETKLHQKYYNYRSHGEWFSLNRCHLKELKEMPGNEL
                     K"
     gene            complement(24805..24957)
                     /locus_tag="ACHKXP_003083"
                     /pseudo
     CDS             complement(24805..24957)
                     /locus_tag="ACHKXP_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24945..25382)
                     /locus_tag="ACHKXP_003084"
     CDS             complement(24945..25382)
                     /locus_tag="ACHKXP_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7648039.1"
                     /translation="MSRVTAIISALVICIIVSLSWAVNHYRDNAMTYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLR"
     gene            complement(25379..25855)
                     /locus_tag="ACHKXP_003085"
     CDS             complement(25379..25855)
                     /locus_tag="ACHKXP_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7648040.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQFGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKVDSLIAKFKEAGGTVREIEV"
     gene            complement(25839..26162)
                     /locus_tag="ACHKXP_003086"
     CDS             complement(25839..26162)
                     /locus_tag="ACHKXP_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO7648041.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGDQ"
     gene            complement(26597..27220)
                     /locus_tag="ACHKXP_003087"
     CDS             complement(26597..27220)
                     /locus_tag="ACHKXP_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7648042.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNTVTR"
     gene            complement(27217..27405)
                     /locus_tag="ACHKXP_003088"
     CDS             complement(27217..27405)
                     /locus_tag="ACHKXP_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO7648043.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(27402..27764)
                     /locus_tag="ACHKXP_003089"
     CDS             complement(27402..27764)
                     /locus_tag="ACHKXP_003089"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7648044.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            complement(27761..28051)
                     /locus_tag="ACHKXP_003090"
     CDS             complement(27761..28051)
                     /locus_tag="ACHKXP_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO7648045.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQSILLKEGKIKS"
     gene            complement(28044..28256)
                     /locus_tag="ACHKXP_003091"
     CDS             complement(28044..28256)
                     /locus_tag="ACHKXP_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648046.1"
                     /translation="MSDYLKWYLCHRWLIKFAVKDWMAADANKLKQRKDYYYARMKEN
                     YCSIRTRIFIKKDLQSILQLRGKVNG"
     gene            complement(28249..28458)
                     /locus_tag="ACHKXP_003092"
     CDS             complement(28249..28458)
                     /locus_tag="ACHKXP_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO7648047.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCSDCCAELMSDPNSSMYEEEDDE"
     gene            complement(28418..28819)
                     /locus_tag="ACHKXP_003093"
     CDS             complement(28418..28819)
                     /locus_tag="ACHKXP_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648048.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKAKTTYIKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            complement(28822..28997)
                     /locus_tag="ACHKXP_003094"
                     /pseudo
     CDS             complement(28822..28997)
                     /locus_tag="ACHKXP_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            complement(28994..>29242)
                     /locus_tag="ACHKXP_003095"
     CDS             complement(28994..>29242)
                     /locus_tag="ACHKXP_003095"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7648049.1"
                     /translation="MLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGHGVRRAA"
BASE COUNT         7083 a         7414 c         6609 g         8136 t
ORIGIN      
        1 ggtgcctgct tttccggcaa caagcaggaa tctcagtgct gaagcaggaa ttagaggtaa
       61 aaacgcccta ccgggccatg aatcacccgg tcattggagt agtaaccaaa gcagatttag
      121 ccagcatgga acaaatatct ttggtgaaaa gttggttacg ggaagctgga gcgcacaacg
      181 tgttagtaac cagtgcagtt aataacaatg gagttacaga actttttgcc ttgctgcata
      241 cagaagaagg ctgttgttaa tttcgattgg ttctcagatg agaatgatga cggtgataca
      301 cagataagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat aaaacgggaa
      361 ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg
      421 gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc
      481 acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatcccag gcttgatgta
      541 atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct ttttctcaac
      601 gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac
      661 gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga
      721 ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc gcagatgaca
      781 atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa
      841 gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa
      901 ctcaagctga tgcgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac
      961 taccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag catcgttcaa
     1021 tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat gaacaaggct
     1081 cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac
     1141 gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca tacaaaccag
     1201 attgcggtta atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct
     1261 ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct
     1321 ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata
     1381 ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccagat
     1441 accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc gccataacgc
     1501 cgtattaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc caatcaataa
     1561 accttgatac ggtttcttgc ccagcgccag ccaaccgcaa agaaacatag ccgccgcgca
     1621 ccagactaac atcagcggta acgagattaa ctccagttgc agagcgataa gacctaaaat
     1681 cgaccccaac accgtaccgc caatacgttc gaatgcgcga gggacaacgt tcccccagaa
     1741 cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt ccgggatagt
     1801 aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag taccatgcac
     1861 aatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat cggcacgcac
     1921 gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcgac cagaaggcgc
     1981 gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa
     2041 aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtctt cgcgtcaaca
     2101 caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat ttcgatatcc
     2161 cagtacccat cttccgcgcc attgttcaag taaacctcga agcagggctt tggtaacatt
     2221 tcataagcac tgtcctgcaa gaggctatta aagaactgat accagggttt agcaaaatca
     2281 tcaccgacta cacgagccac cgccaccgca tattgaccac ctgaaatttc tgtcagaatg
     2341 acgccctcac tgttttcggg gagcgtaaag ttattcggta ccgtcacgac ggtgtcgcag
     2401 cgtaattttt cggcgggtgt ttcatccgga ttgtcgtaat agacagcaac ccactccttc
     2461 ggcacaatat ttttgctatc tacccacatc atcaactgct caaaaccttt ctttaccgtc
     2521 tgttcccacg ggccaacgag atggaaacct gcaacggtac gtttatcttc ctgcttaatc
     2581 tcgtagttca tgacgcctcc attaacactg tttttatata cagtatagtt gcaatattaa
     2641 aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca gtctgctgca
     2701 aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca tgccgccttc
     2761 cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac ggtcgtaaag
     2821 ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt taccatcaag
     2881 ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc agaactcttg
     2941 ttccgttccc aggtcgatat tttctttatc gccataccag atgcgttccg ttccgacctt
     3001 tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac gcagtaattt
     3061 tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg caatgagacg
     3121 acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag tatgacccgt
     3181 tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt tttgctgggt
     3241 gataccgttc caggtgagac ttttctcact gtacatatga taaaactcgg gctcgccgtg
     3301 gatgatagcg cgagaaagca cagctaaatc ataagccgag ctatgctggc caggtgcatc
     3361 cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg catagttgtt
     3421 catcatttca acaaactgcc gttgcccacc ggcaatatag tcagccagag caacacaagc
     3481 gtcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata cgcgatcgcc
     3541 ctctttcaaa aacatcagtg aagaaccgac aaacaccgga ttatctttcg cccacgcatc
     3601 gcgccccacg gtgacaatat cgtctggcgt aatgcgatga ctatcgatag cgcgatccac
     3661 gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat gctcattaac
     3721 cgcggtgagg atctgactgg tggtgtaatc catcaatacc caggacccgg catgaatctc
     3781 tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata agttaaaaac
     3841 gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag gagtttcaaa
     3901 tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca agaaaatgtg
     3961 actaccctct catttttatc tgacatgatc tgttgccact cgctgccaaa ttgtggcgct
     4021 aaagctgatt agcacggtga tatttgatac tctggcagac agcagaaata acggatttaa
     4081 cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacaatt acgcgcaaca
     4141 cccctgagag ccgccatgaa caagggtttc atgatttgtg attttagttt ggtacgcaat
     4201 ttggtacaca acacaatttt cacttcagcg ggtagtcatc aaactcacca taacgagcat
     4261 cgttgatgat gtatgtgatc accacaaata tcacatctgg actagttccg tcttcatgcg
     4321 caggtatctg ttcacgcctc cctttctgca aatcctaaag atgagatttt gggattttgg
     4381 gatatctttt tatcctcagt tctctatcca ttctacaaag cagtaatgaa ccatcaccgt
     4441 gtgatagtga ggcatccgct acgttaagcg cagagaaatg atgcagtggt ggtcggactg
     4501 gattgatgaa aaggtgtagc aaccaacatt gctatatctc agatagcaca aagccttgca
     4561 atccagtgca aagctttgtg tgtctcagtt ttgtcttatc aaccctattg atgcaataag
     4621 acaaaaaaag gcattaatga cacaagtgaa taattagaaa atgtggccgc aacacttaag
     4681 atgcggcctt caatgcatta aagaggtgtt atggtaaatg ttacatttgt ctggcttgca
     4741 ccaagtgtta cttccaaatt cccaccacta acgctgatag aggttatcga aattccagag
     4801 ccaccataga caacctgatt agcggtgttt acagaaagcg tagaaccatc atagctgaac
     4861 gtaaccccgt cagcataaga ttgatagtta ttgacgcgta ttttaaaaat ctccctataa
     4921 actgacgcgg ctcctaaaaa actagggaat gcgccattga tttctgtcag tgggatttgg
     4981 aaagttttcc caggtccaga aaatcccttt ttgtgttggg atactctgaa tgtctcatag
     5041 gaaccagttg acagtaactg ccctctatat gctgattctt tatgaaatgc ttgtactgac
     5101 tccgcataat ttacgacacc tctgccaacc cttcctgtat caatttctaa tgttgaacca
     5161 gtatccacta aagatgccgg gaagttaaat gtggcaccat tccagaatgt tattcttgga
     5221 actatgatgt gggtatttgc acctgtaatt gtaaataagc gttggcgttg tgtgcgtaga
     5281 gacaggttca aaacagccga cgctcctccg ctagccagta tcactgcatt tgtagtatct
     5341 atagtgtcgt agcagttgaa ggtcagcgtt gccgaaccag ctaccatatt tatgtacgcg
     5401 ttcgctgacg gtgacggact ctccgcacca atatcaccag caacgtcaca ctctgaaaaa
     5461 taataagatg aaccaccaga aacactacct gcgttatcgc atgccgttga ttgaaagttt
     5521 atgtactggc atctttccat atgccatcca tctaactcag cgccggccac atagcagctg
     5581 cttatatgta gacttgttgc tgcttggtta actgctgctg ctggcgtccc gcctgtagag
     5641 gtaaagcgtg ctgcgtcata aaagacaccg tgagatgtat tctcagagaa tcggcataag
     5701 gtaagcccac ctaaccaacc gcttttaacc cacagaccat gtttacaacc tctggttctc
     5761 actttctcaa tggagtagcc aacaacctgc ggcatataaa taccaacagg tcggttgtca
     5821 gatggcgcta aatgacagtc aatatatcca ccactgaatg tccagtatcg gcagctatca
     5881 tttccgccga taaggaagac gcagttaaca tcgccttcta cgtaagctcc ttgcgtggga
     5941 atgtatgcat caagtccagt agtggtagtt gatgttttaa caattgtaga ttcgccgaga
     6001 tttaccgacc aatagtcagt agcctgcaat aaaacaggtt ggtctataag atagccacga
     6061 cctttttcgt caggtgtttt aatcttacca agagaatcga acatgttctg gaatgcagcg
     6121 gatgaggagg ttgctccaga cggtaaagag ccatcatcct gaacttcaaa ggacactgcg
     6181 ccgaagtcat ctacagttac attttcttta tttacccgct cccagtacca catggatgac
     6241 accacagcga ttatgccgcc gtcatcggat ggaatattag acattccaac tgtagagcgg
     6301 gcaataaatt caccaccacc tgtacggcct ccagagtagt aagatgccag aagaattctc
     6361 tgacctccta cttctggaac aatggttcgt agttcatcaa aagaatctac ctgaccgatt
     6421 aacttaaatc catcaacgcc agaaagacgc ttctcaaatt ggtcagggtc atacttcagc
     6481 acattcggaa aatagaactg ctgcgcacca tatgcatcat aaacagccat agaatggcct
     6541 tgcacggtaa cgaatttggc aatctgtccg ttatataccg gatatccggc agcgttaatg
     6601 atgattggtt gagatacagg aacgtgagaa ccgtcttcat tctccacata aacctgaatc
     6661 cggttttcag gatttaccgg gtcagtgtca attttaccga tataaatttt gccattggct
     6721 acggctttaa aagaacgagc catagtgaat agttgcgaag gcatcgatac gatcacattg
     6781 gctgtaatgt ctgtcattta atttgctcca gatgcaaaga atcgccgcag catggctacg
     6841 gtgaattttg ggcataaaaa aacccagccg aagctgggtt ggtttttatg aaaagattaa
     6901 attaggctgc ggtagaaaca ttaattagat ttctcaatac ttcaactcct tttaagttgt
     6961 agcgaaacgc ttctacctgc ttgctggaat gcgcggattt atccaagaag aacttcccat
     7021 actgctcagt tttgaggttg tttgcgttag caatgcgacc aatcttgttg gccgttactc
     7081 caagctgctc tgcaacctca cctgctgagt agtaatgctc ttctattgcc ggaagaggta
     7141 ttgcattaaa accaacgagc gggttgatta tacttgctgc cgctgtctgc tttgcctcag
     7201 gcgcaagatt tggcatcaga tcgaacagat tggtaacagc ttcaaccgtc attttcaatg
     7261 ttcgcgcttg acgatactca acaagtccac tcgctgattt accgctttta atgtgtgctt
     7321 cctgcatact ttcaagtttg tctacaagtg ttcggcgaac tgctttagat tcgcgagcag
     7381 caacacgaag cgcctgcttg atagtcatgg agataacatc aatgtcagcc ccgtttttcc
     7441 gacctacact ttttgtgtag gtctcgcctt ccaattcatc ctcaattttc tcgatgaatt
     7501 tgttgttacg tactgatggc tctccacata acttgcgagc ttcattaacc atcatcagaa
     7561 gactttggct gtcaatggtt ttatccgtga caacagatcc gatgtttgct acattcttaa
     7621 aagtcattag gcattcctta tgtggtagta agggtgtgac ataggccgcc agcagcacac
     7681 tggcggtttt ctttttgcgc cgtccagcgc accaatcaat gaatccattc ctcgccgcga
     7741 agttttgcca gcattggctg agcgttattt acgacaaaat tgttggtatc aagattcttc
     7801 atttcacgaa gaagtgattt cttggtttct tctgacatgt agcgagtctc atatgcaata
     7861 tcgtaaatcc ttcctgaaag ctcagaacca atttgcttca ttcctgggta gatgtgtttg
     7921 cacatttgtt gactcttctc catccacaat tgtaagtagc agagattaac cagttcttcg
     7981 tcagtaaact gttttgcaat cggtgagcat tctgcctgcc gatccaaaat atccagcacc
     8041 cagcggcgga actctttggc taccggagtg cgagcaaaca tcgcgattag gtgggcaccg
     8101 cgtagtgaga aaactcgcac ttttttgcga taatttcctg aggtactcac ttcgagtacc
     8161 tgagtcattc cggcgctaaa ctcatcgcta tacttgttat aaatcattgt tactgcacga
     8221 ctatttgcgt atttaagtgc agatgcaata ttagatgatg taaaccaaac accatgcata
     8281 tcacgggtgg gcaccaactc aactccgtgg aagttgtaat ctgattttgc tacaatattc
     8341 atgttagttt ccttgcatac ggttactgac atagaggccc ggtttgtgtt cgcgcactgc
     8401 cgggcttcac tatttttact gggcattagc tctttcctct ctcaggcttt tagccagtcg
     8461 ctgcacaatc gcagagttga tagaaattcc atccatttca gctacgcgtc tgatctcctc
     8521 cttcattcgc gctggcaaac gaagctggaa actctgactt ttacgaccac tgtaaagaac
     8581 atcgctcatc atcaatatct ccgtcataat gacatcacca tgatgtcatg acaccattat
     8641 gatgccattg ctcataatgt caatatgatg ctactgtgtt ttttattgct catatgcctt
     8701 gcgaataaga aaatgtcaga aaaagacgaa tcaaatttca tagaaagatt tacggttaga
     8761 atgcctgacg gcatgcgtag tgctattgcg gaaagggcta aacgaaacgg tcgatctatg
     8821 aactcagaga tagttcaaat tcttgaggat gcgttatcat atgacgtctc atcagtagac
     8881 aaatcattag atcttaaaca aataaaatct ttacttgata aaatttccaa cgagttactg
     8941 gatagaatcc caaaagacac ataacaaaac attatcatct ggtatcctgc gcaaaactaa
     9001 ggaggttggt gtgtatacaa cagttatcgt gatagctatc gcactcattg tggttcagta
     9061 ccaactggca tctttaaaac aaaagatatc tgacctcaag actgagaacg aagcacttaa
     9121 aaactcaata aaagatgaga agaacaaact attattcact atatctgata ttgagcaatc
     9181 cattgaaatt attgagaata atattaacag attaaaaaaa gaagatattc atgaaatcaa
     9241 tgacaatatc aaggatttga aatcttggct taggaatgta ggacaaatcg ccacatcaac
     9301 acgagataag ctcaatccat ccatggataa ttaattactc ctgtgccatt ccgcttagcg
     9361 acgctacaat gccagccctc gctaagcgat tgaactcttc gtttccaact gcatcacgta
     9421 ttgcttttat ggcggcttta tttgccataa atctgcgttc cgctgccgct aatgcttctt
     9481 tgcttcctcc tgctcttact gctttagtgg cttcctgaac tgctttctct atcgcatacc
     9541 gaccactacg tgtggtggca attttagata cagcgccttt taacccagcg ccaactaaag
     9601 cacctgcggc agcgcctgca atgcctcctc cagcgccacc aacaatggca cctgatgttg
     9661 agttggcaat tgcatttaac actgttgatg tgacgttgga taaaccagca tccagatcgc
     9721 gtagtacatt ggcagttctc cctgttcttt caatatactg ctgaggtttc actgctgctc
     9781 ttgcaagagt gccatacgca tcagcaattc ttccaagctc tgaggaatat ctgctaatgg
     9841 cttttacatt ttgtggagtg agtatctctg cgatatggtt aattcctgct gcatcagctt
     9901 tgccaccacg tacaccatgc gagatggcat cctgcaacat tgatgatata gcaggaacac
     9961 gctctgattc tggcagcgcg cggatcatag aatggaatcc agcaggacca ttaagacctt
    10021 tagctgacga tgattgaagg gattttactc cattcgtaat cagtgcatct gttgccaaat
    10081 cacgcccgaa aacagactct gcactctctt gtgctgataa cctcgcttta gacagatcat
    10141 tagctttttg ccagtcatca agaaatccgc cgttttccgc cattgtgcgc atatcatcag
    10201 taattgcccg gcgtatttcc cctgctctcc ttgccgcatt tgcctctccg ctacgcttat
    10261 atttttgctc cgcatcagca aatttcgctc tccatgcttt catgccatca aatgttactc
    10321 caccttgatt gtttgcctga acaaactgtt tcatttcagg agtaagcggt atgccagcag
    10381 atcgctctgc ctgaataacg gcattaccat ttagcattct tgctttttga tttggcattg
    10441 ttgaccgcac gtcatcccat gccgcgcgct cggcatcctt catctgatca agattttgaa
    10501 gaatcctttg ttttatagcc gcactttttt ctgatgccgt tccagatgca gccccaaatt
    10561 catcaaggtt tcgacttaac tttgatgata tttcgttaaa tgctgcctga tgggcgtcct
    10621 gaacaattcc aggtgttgat gccaatgcgc cttcggcttg tgcaattcca cgacttccag
    10681 atcgcattcc tggtgttaat gcgtttatat caattccagc agactcagcc gcttttgcta
    10741 catcttcgga cacattggcg gcctgactgg caattgactg acgcccagca cctgactttg
    10801 ccatcctgga aacatcatta gcagaattca gtgctgcacc accaagagcc tgtgaaaccc
    10861 ttggcgcaat aacgcgcccg acacctgaaa gaacgccttg agcaccaata ttgataccac
    10921 cgttaatggc agcattttgt gcaaagtcgc cctcctgatt tgcagcatca gcaagagaac
    10981 ctgcaatcat gtttcctgcg gaaccgatat ctccagcgag ctttgctggc gctccagcag
    11041 cttttgccgc tgtgccaatt ggcaggagat acccaccaat tgtttcaccg gcttgcgcgt
    11101 aagggtctgt cggtcgatcg acagggcgat aaacatcatc caaaaccttg gggccgccaa
    11161 gcccctggct gattgcatta atcagacttg cgccaccctg caatacgtca aatggtatgt
    11221 ttaccagacc acgaccagcc tgttcagcaa tttgccctgc actttgacca cctgtgagcc
    11281 aatcaccagc ttgttgcatc aatgatggtt cttctttctg ctgctgaggc ggagggtatg
    11341 ctgcataaaa ctgatctctt gcttcagccc atttgtcacc agccttaggg gcaacaacct
    11401 catcaaaata ttgcgcttga gcctgtgctt tctgttcttc agttaacgcc tgatactgtg
    11461 gagaagcgat aacatctttc catgctttag ccattaatca ccccataaag acgagaaacc
    11521 ggacttattg ctgtcgcttc ctgattttcg ctcactaaca tatgtgtcat aacctgatga
    11581 actatatccc attgattcag cctcccttgc tgcaaccttt tgaaatacag aatattgcga
    11641 tcggatttca gataactgtt ttctgacgac ctcttcaggc tgtgttatat cgagtttcgc
    11701 gatcaggttt tccagttttt ggccttcagc attggagagg ctacccatac ctcgcatagt
    11761 ctgcacgttc tggacaaacg cacccgactt taattcttct atcgcattac ggtttgcaag
    11821 cccttcagca cttgtgaagc catctatatt tcttccttcg aagcgaccga taccttcaag
    11881 ctccttctta ccaagcaaag aatcaatttt ctctatccct cgctcaccag taatcaacgc
    11941 attgttgtaa ttattgttgc catcaagcca tctcttagcc tgagacattc tggctgacgt
    12001 tgcagcttta ccggttagcg gatcaattcc cgtcgctgct atctgtgagt taagagacaa
    12061 aacatccata tcctgaagtt gtcctgctct ttcaagggcc gcctgtgact gcttaaacac
    12121 atacttgtcg tgattcagtc ttgccatttg agccttatag gaaagatcct gccccctaat
    12181 agccctcgca ttcgtcatgt cattattgcg aatggtttcg ttaattcttt gctgctcctg
    12241 ctggcgacca accatcttat cctgaacagc aaacgccttt tctggtccaa gcgcaccgag
    12301 agacatagta gtcagcatgt gtgatagctg ttctggattc tggatacctg tctgaatcat
    12361 ccagtcagca ttcgcaccca cgcgatttaa cctgtccttg ttgtcagtaa tgaatttact
    12421 gtaggcttcc ggtccctgag aaagagcgac gttagccctc atggctaaat cgcccatatc
    12481 gttgcgttgc tgctcattaa gaccggaaaa cgcctgttgt gcctgtgcaa caaacgctgg
    12541 attttcctgg gcaaacttaa atagtcccga tggatcacca gaagcccatg catcagcgtg
    12601 aaccttattg aatgcactaa tcgctttctg ttgctgttcc tgcttataaa tatcagcagc
    12661 cccagccaga ccacgtaacg cggtcagacc aacgttattt gcacctgagc gagccagttc
    12721 attgttttcg cggatcagac caagcgttgc gttaatgtcg cttgcctttg gcgcattctc
    12781 attttgcgta ccgatgccag ccagaaaacc accagaatta ataccctgtt gccacgtagc
    12841 catgattacc ccttaaaaca acgagccaag cagaccaaga ccagcaccga taccagcacc
    12901 ccacggagtt gatagctcga gagcactggc tatgccacca cccaaaagcg caccggatgc
    12961 agcaccacta accccctgct gcaatgctga cggtcggttg gcgtttgccg cagccagcgc
    13021 cgcgctttgc tgtgaaatct gactcatgtt gttggcatat gtttgcccgg cgtttgcctg
    13081 cccctgaaga gcgccaagac cgatatttgc caggttgttg taattgttca tttgtccaga
    13141 tagccattgc tgaccaagcg ttggtgcgat tgttgctaac tgattactgg ttgcggtgga
    13201 acccaatcca cctgttgctt ccgctgccgc cagactctga tagcgagcct gaccagcaag
    13261 atctttgtac tgctgagagt tgtaatactg gttaagcgcc tgaccttgcc cctccagaga
    13321 cgataagttc tcgaggctgc ctagatactt atcagccaga ggagtaaacg gcttcaggtt
    13381 gttcatgatg gtgttgaact gcttattttg caggtctgcg gcatacttct gagcttctgc
    13441 ggcatacttt gcacttttat cagaactgcc acctttcccg cctttttcag ggcaataagg
    13501 ttcctcgccg cgcagttttc tgcccagctt aaatgcatat aacatggcta tctcccgtga
    13561 ttcaggaagt cgattagttc ttcgcgtgtg gcgctgtaaa aagtcacgtc atccacgcct
    13621 ttaaagtatt tcttgatggt tccgacacgc ttaaggccaa tcattgcgca gtaaatctgc
    13681 ccgtggcgga atttgcgtgc ggcgaacgat gtgacgcact gaacggtggt gttagtcaga
    13741 atgtatcgcc agaacgccag accgatttcc ttgctgaagc cgcgaatctc tggcaggtac
    13801 atggcgtggc aatcgaatgt ctgcggctga atctcctgat agtaaacaat gccgccaaac
    13861 tgcccgtgca cgttcacctc aaagtaacgg cattcaggtt tgtagtcgta tccatcgccg
    13921 ttgttgctac cggcgataat gtcagggtga tttcctacgg cttctatcag gtcgatgttt
    13981 cgcgttggtt tgaatgtaat catcagtcaa tcagcccatg taatctaagt gccgtttcaa
    14041 gcgccagaat acgctgccgc gcctgctgca aacctgttgc gagagctgcg acttcggatt
    14101 gcgtgtacgt agtgccgacc gtgtatgact ggttagcgtt gaatgagcca agaagtggcg
    14161 tacctgtggc tgcagtccat ccggtatttc ttgctccaac aacctgaatt ccatcaactg
    14221 aatatgatgt ttttacatcc agcggtgacg caagagactg cgattcggtt acagttttcg
    14281 atacgtaatc actcttaatg tcagatacat cgctttctac gccatccagt ctttggtcaa
    14341 cagtgaccag atgcgcctga atatcgataa cctcatccag caagtaatca acatcgctac
    14401 gcagtacgac tatcttccct tcggcagttg ttaacctgac ctcaagtaga tttatcgctt
    14461 ttgtgtttgc ggtgattctt gcgtcgtgat cagccagttc gacgtcctgt tcatcgtttt
    14521 ttacctgagc atcgtaagcg ccctgaccag cctgatttgc cttcccggca attgcaccga
    14581 catcagctcc ctgatttatg acatacagca ggtaagactg gctgaatata ttgcgtggca
    14641 aaattgaagc atcaaggcgc gtagcctgaa ccgcgacagg attattcagt gatgaatcag
    14701 ccattactca atccttatct ggcagccaga cagagtgaca ggtgacttcg tgataacgcg
    14761 caatttgaag ccgacatttt tcctgatgcg cccgactcgc ttccacaaaa cgcgtttgtc
    14821 gtaaacgaac ggttcattct gctcaatcat ctgctcacga ccgtaattta tgccgtcagt
    14881 ggttgcagag aggaacaggc ggtcggcgta ctgcgcaacg ccagttgacg attcaacctc
    14941 aaggtcgaaa actctggcgt tatccgcttt gaacaacgga gtaaacagca ggtgttcctg
    15001 ttgcttgtcg tactggctgc tgatgtcgaa ttgcagtttc ccggtaacag attccagctt
    15061 atcgccgcac gttatctgat tgccttcgta aatgaagtcg atagcgcggt acacatcgtc
    15121 atacaagcca gttttcaaca cacaccattg cggaccattg gcgcttgaag atgcgtcgta
    15181 cacgaggacg tgacgcggaa gatggataat cagcaactca tgagcatcaa atcgcagcga
    15241 ttccatcaca ccatcagcca gttcatcagc agtgtaggag cggaggattt tctcaatgct
    15301 cgcgctggcg attggtgata cctgaccgga gccgatgata tacacagacg gagcacctgt
    15361 tgccggattg ctgatgaacg cataagaatc agcgaatggc gttttgcagt aagttccggc
    15421 aatgcctttc tgcaccatca gcgatggctg ggcgacatac aaagcagcac caacggtggt
    15481 tgcaccagtc agggaaaaat actcaatcgt cgatgaacca aagcagacga tgaagtctcg
    15541 ccatgtcccg ataccgatga tgccgtccgg ctgcgattct gcgcgatatt gtgcactgta
    15601 tcggtcaggg tgcgattcgt cttcaaggtc agtgataaac catgaatcag ttccgtcttt
    15661 tgaccacgca taacgcccac gtaagcgcgt aatgtcgcga actgaaccta actcgtactg
    15721 tgtgaatccg ctgtctgtag gccagtttga gacggtttta accgtgccat catagcgata
    15781 ctcgaccagt tgaccattaa cgcctacagc ctgagatgtt cgaccatgcg ccattgatac
    15841 gcgaccactt ccggcaacat caccaacctc gctttctcct ttgtacagtt tgccgccaca
    15901 cacacgatag acagcattct gcgccatgtt gtactcgacg cctcgagata caccgttcac
    15961 atcagaacgt ttggcaatgc ccgggaatga gcgaagatat ccgctgctgt tcaggatttc
    16021 tttgggtgta gccagcatat tcactggcag atagtcgata tagtcggcat ttcggaagtc
    16081 tttgccgaca cctttcataa gcggaagttg ctgaataggc atttattcgc ccccgttatc
    16141 gcaaggttcc ttccggtgga agtaattcca accattccac ttcgccaact ggttaccgct
    16201 accaacaggc atacggtttg gataaccgga cttacattta gcggcttttg ctctgtccat
    16261 tgcagacagt ttgacgagtc gctctttccc gtatctggca gtggttataa gttttgctgg
    16321 cgcttccagc gcataatctg gagcaatgcg gcaggcaagg ttgaaaatga cggcattgat
    16381 agcgttattt gataaaccgt gctcatcgcc cggttctgga gcgacatctg catcagcgaa
    16441 aatgtagcca acgttgatac caggtgacac atcaccgcca agccattcag ccatcatcat
    16501 ttcaaggtcg ttgacgccgt cttccataga ctgcggttct acatcggtta acgtggcatt
    16561 tgatgccaca ccgagcttac gtaatgccgc aagaactaaa tcacccttcg ttgtcaggtt
    16621 catctgctgc cgccttaggt tttcgaccag gctttttacg ctgtttttct tctggctctg
    16681 gctctgcaat agccggacgc aaactcagga gtcgtccaag aacatcattt gcttcatgac
    16741 catcccactc tttcccgaac tcaagctcag taccttcagg aaggaactcg atttcttcaa
    16801 caggtaggtg ataagtgatt tcgccttctg gagtggtgat accagcaatg atccagccat
    16861 cccactcttc accgtcactg tgtttgcgag accaccacga aagctcagcg taagcatgca
    16921 tcagagatga gaagaggcgc actcggtgag cgtaaagctc gttaaaagtg tgataaccgt
    16981 cggacacttc gcccatatca actggggaag tttcacctcc gccaacactc ccaatttgat
    17041 caccaacaag agaatcatca ggaacatcgt cagggtgctt ataccagcca tttgctaagt
    17101 gcacagctac atcatcagga tcaacggttt tcgttttcag cttgcgcccc cagattttgg
    17161 tatctccgcc agcctgaaaa atcattacgc tcattggtat ctccaataga aaagggagcc
    17221 gaagctccct ctggttatca cgcggtctgg ttaggcagac caacaccaat tgcctctggt
    17281 cgtacagcac atgctgaata ccacacagca atacggcact taccagacag agtgttgata
    17341 tcaccctgcg ttgcgaagat accgttaaca ccaataccag gaatgctgaa ggaagacgtt
    17401 ttcataccag caaacagttc atgggttacc gggatcggct gagacagcag acggattgag
    17461 tcatcagccc agaacacgtt agcggtggtt gttgccacgt tcagaacgtt taccggagtg
    17521 gtatcagcaa gagatgtgtt tacattagcg taagccttct cttcttttgt cagtgacgcg
    17581 tcatccagtg caatcggttt cggcgtgatt tcgatgtgag taccatcgat cacacgggtg
    17641 attgagaaag tcgcatcatc agtcagcacg ttcttcgcca tctgagacag gaatttcaca
    17701 ccagtgaagc tgattttgtc gccgcgctta aatccggtgg tggaggatac ggtcaccgtt
    17761 gcaacacggt tgtcgacgtt ctctttgtta ccatcggtat caagggtgta tgcctgcggc
    17821 ttaaacttct gcgcaccaga aacagttaca ccagtagcgg ttgacttggt aactgccgga
    17881 agtttcggtg agcgaagaat ttcatcaaag ccagcaatct gacgctgaat agtaccgttg
    17941 cgatacgctt cttcaggaac gcgcccgaag atgtcaccat ctaccaggtt gcggcctgct
    18001 ttgcggtaat cgtcagggtt caggaagtaa ctgatgccca tgtcgcggtt gagttcgcgg
    18061 gagaacatca ggcgctctgc atcagacaca aaatcccagc cagacaggcc agtagatgga
    18121 ccaattgcgc gggtatcgtg aacaacaagt gagcccattt cagttgcctg tttggcaatc
    18181 gctgactcaa tgttattcgc cagttttttg gcagatgcct ggatgcggcg acggtaagaa
    18241 cgctcatcac gcaggtcatc tgcacgaagc tcgaagaaat cgttatccgg atcgcccatg
    18301 ttgcatttca cggagagctc cagaatcccg gttgcattgc cagttaaatc ccagccagtc
    18361 tgggttggcg cttcctgctc aacaggcatc cacacggtgt tgcttgagcg ctgcatggat
    18421 tctgccggag gggtgtattt tgtcactttg gacgccattg gcgtcagatt ctggacggtt
    18481 tcgatgattt catccagagc atacgtgacc agttgacctt catttaatgc cattatcgaa
    18541 ttcctttatt cagttgcgcc ttgagcttgc ggtatgtctc tacatcccct ttgtttgctg
    18601 ccgcttccat ctgcttttca atcgcagata tatttgcagc aacagcgtgt ccctgaatgg
    18661 gttcatcagg tagcggggct tctgaaacag gtttggctcg aggcttgaga gttaaacgtt
    18721 ctgaaagtcg agtgagttca atcagcgcgg attgcccgtc catcgccagc aactggcgtg
    18781 ttttctcagg attagcacca aggtgataca tgagagcggc ggatttctcc gggaagaggc
    18841 gcatgatgtc ggcaccgact gctggcggca ccagttgcat gaatgcatcc tctttctcct
    18901 gatagtcagg gatattgagc ttttccgctg cgtcgtagtg cttacgggct gcctcgacgt
    18961 attgcgctga ttgctgggtg aactcctgag ttttgcgacc ctgctcggcg acagcctggc
    19021 ttcgtgcgtc catagccttg atctgccatt cactgtttgc ctgctggaag gcagccagtg
    19081 cgcggctctg gtcatagtcg aacttagcca gtgcgtcttc ggaaagataa tcgttagggt
    19141 ctggttgttt tggtaactca gggttcaccc gcaggtgctc cggcaactct ccacgcttaa
    19201 ccgcttccat ctgctgctca agctcacgct ggcgtttgcg ttcgatgcgg cgacgggcaa
    19261 attcagcatt agttgccggg tcttgttttg gtttctcatc gtctttcagg acaatctcga
    19321 agccttcttc ctgacctgcg ttgtcgttgg cattatcgac aactaagcca tcagcagatg
    19381 ccgctgcatg attgccgggc agggttaatt cttcagaagc ctgaatgtcg gtggtttggt
    19441 ccatgattaa ctctctctta ttgaggtgtc tcggctactc cgccggaggg gatttgaact
    19501 tgacgcataa gattcgcgaa atccatgcgt tgtgaatgag tctggtctgc atctttaaga
    19561 agcagctcag cgttagcacg agcatctttg ctgcgctgtt gctggaattg acctacgagc
    19621 ttgaggtact cacgcagttc tgcctgcttg tcgaggtcca tattgttgaa gatttctgca
    19681 atcttcgcgg cgttgagttg gttttgggct tcaaccttgg cggcttcaac ctgaatctgc
    19741 gcctgttggt tctctgcctt gagcaattca gcctgacctt gcagaaggat accctgcgcc
    19801 tgaatttgct ctgctgatgg ctgctgcggc tgctgttgag cctgctgtac catctccatc
    19861 tcttcaggtg tttctggttt cttcagcccc atcatcacca gttgcttgtt cgcgtactct
    19921 cgcatcatct cgacgccttt accgtcaagc agcgtgaagt attgcagcat cagcatctgg
    19981 aactctggag taccttgcgg aaccttggtg agcaactcct gaatctctgc gcggttctgt
    20041 tccttcatgc tctggaagga tggcccaacg tccgtatagc actcatagcg accgcgaatg
    20101 tcgttgagtg tgaccacatt gccggactgg taatcgacaa cttgcgcata gagttgaacg
    20161 tctttctcgc ttccatcttc gagtgtcagc gttacatgac gaggaacgtc ataaatatcg
    20221 ttgaccattg aggcataaat ctcgccatca cgtcgcattg cggtagccag gttatcctga
    20281 aacacgtatg tctcaaggtc tgcccgcatg ttcagttgat tgacggtatc gaaagctacc
    20341 tgagagtttg cttcctgcgc atccacacca agactagcca cctctttcac tgcgttggtg
    20401 gcagcctcaa gcatgtaagc gttggcttgc ggcacttcag ggttttccat gtaggagatt
    20461 ggaccaatcg gcaggtcgtt accgttttca tcggtcctgt tctgcagata gtacggatag
    20521 tcatcatttc caccgtacat gtattcgtag ccttcgattt gctcagggaa gaaggtcggt
    20581 ttcttcttcg gtgaacgagc aacaatatcg gcgttgaacg acatgatcat gttacgaagg
    20641 cgttgaccgt ctttcgtcag ccttaccacg ccctcgtagc actccttgtc accagcgaat
    20701 gaccattcgc cgtacacagg aacgattggg atatgctctc cggctatctt ctcgcggtct
    20761 ttcagtatct gcgtgcaggt gatgatcgac ttatacacac gccgacgctt gaccttacgc
    20821 tctgctacct taatgaatcc acgattagcc aggtcgtcga tgacgtcttt gatatcctgc
    20881 tggtaatagc tgaccggctc acctgtcagc gggtcgcggt agatgaagac tttctctttc
    20941 ttctcttcga cctcgtaata ctcagcgacg tagacgacat cattcgatac ccacggaaac
    21001 agccatgtat cgtttggatt ctggaaagat ggcaaggtgt ccgaatcaat accgtaatcc
    21061 tctgcgaact ctttccagcc attgcgtgac aaggcgttaa tcaccgtgca gtgcttagcg
    21121 tcgctcttat ccatctgctt gctgtttgcg tcccatatga cgtgtgagca ggcttcatgg
    21181 attggcaggc gtcgaattac ctgattgttg cttgttgggt cgttgtcttc gtactgtgtg
    21241 accagacgcc atgcaccaac gccggactct atctgctcac gaacgccaac gttaacggca
    21301 atctttgccg tgttatggcg catatcagta cgatacatcc ccatcagcac atcggcagca
    21361 tcaggattag caccgtcttt tggtcggaag agaacgtcga tagggttccg gcgcatctct
    21421 gcgaccaact tcctgacaac cgggcggaca acatcgaatt gtccgcgata ttgcagggtg
    21481 gtgtagtttg atagccagtc atcccattgc gacactcggc taaaatacag gtcatttgtc
    21541 gcctcggttc tggcttcatc gctcgccatc cagtctgcgt caaacttaca cagaatggaa
    21601 ttgagtctgt tttcgtcggc catttaagtt ctccgtgcga tgggcctgat tggggctggt
    21661 atctttttct cttttggttt tttgatgtcg cgcatcattt ttgcgaagcg gcgcatcatg
    21721 tatgcatagc gaacggcgga tagcacgtcg tcgttaagct tgacgatctt cccgttttca
    21781 tcacggtgat agaggcggaa ctcctcaaag aatggctcac aggtgttgaa tactttgaag
    21841 cgaccatcaa gcatcatgtc gcgcaattca gtgattccag gctccacagc gttaccgcca
    21901 tcaggccatg tcgcatgctc ctgcaacatc ataaaaccag cgtccgcgta ctgcccttta
    21961 agctgctcac cgccgccctt ctcgtgctgg tttccgtcat ggggccatgc ggttggcact
    22021 ttatgcgccc atggtttaac ggctccccac gcctgaacag ctgtcttttc tttcgccttc
    22081 cacacgcgcg aaacgtagat tgtgtctgcg tccttatccc accaaagctg aacctgcgcc
    22141 tgagggtgat cccatccgaa atccatcccg ccaattacgt agaagtgatc aggacactcg
    22201 aacggctgac acttaatcgt ctcttccggt atctggaaga ttcgaccact acccatcgta
    22261 ggaataccgc gagctcgagc ttctctctca tgctctggat aggatgcgat gatttgctct
    22321 ttctgctcgt cggtatagtg ctcagcgtca tagatggtca tgttgaccac tttctgcgac
    22381 ttgctgggat tcttcaggaa cttggtaaca acgtcagaca tccccatcag cggggtaaac
    22441 gtcaggattg agaattgccc gtatttgttg gtacgggtaa gcccttcgcc ataaatgctg
    22501 tatggtggtt cttcgtcaaa ccacactccg tggattgtgt caccctgcca gcgagcacga
    22561 ccttgcgagt atggcttgaa gtagcagatt gaaatgccat cttcaacgcc atcagccgtg
    22621 tgatgcttaa ccagaaggtg atcaacaagg ttcggaaaga aaggagactt cttccagcta
    22681 atgatgtcct ctttcggtat ggagccgtag ccaggctcat cattctcttc gatacgaccg
    22741 cacaggatgc gttgagtcgt tttggttaca gtctcgtttg tctcgccgcc aatccagaag
    22801 acaacaggct catagaaacg cttacctttc cactccccac catatttacc atcagccgga
    22861 tagccttttg tgcccggata acgcccggta aggtgaaacg cgacttcagc agcaccagta
    22921 aatgacttac caagctggtt accagccata aaacagcgct ctggatagtc atgcccggcg
    22981 tcgatgaact cacgctgttt gctgtatggc gtaaattcat atagcaggtg tgtgttccgg
    23041 tagttctctt cttcttcgag tagctcgagc aactcgattt gctcttcgtc gctcaggtta
    23101 tcaagaatcg cgtccagttc cacggttgaa tagctccttg atacgagagc gtcgcttatc
    23161 gcgatctccc ttatcaggtg tcacgtcttc aacttgcgac tgctctttga ggcccaaatc
    23221 acgggcgatg atgttagcgt tgagaaggtc agcggctgcg ccagagaatt tctgatcgta
    23281 gatgacctgc tctgctcgcg taacgacttc agataaatct tctctcaggc gatatgtgcg
    23341 ccatgtttca agcgtcacat caatgaacag agtgaggcct gtaatagtca tcgctcgcat
    23401 cttggcgata ggctcttgta tcacttcacc ctgatacgag aacgccttca tctcccatag
    23461 cgggttagct tctacccact cgaagtattc acaacaagca gcccacagcg cctcaggcga
    23521 ttcgaattta ggatttcgcc catgactact gcgggcctcc caaaatcggt tgccctttgg
    23581 tgctgccata ttcatctcac ttagttgtta tttcaggctg agcatcatgc tccggtagtg
    23641 aacaggtcta acgcttcctt cgatttacgc accgcttcaa atgtgcggat cgtgatatcc
    23701 gaattagcgc cgcctgactg gaagtgaatt ttgaatagct caagcttcag ttcgtcagtg
    23761 ccaatgaact gaaatgcttc ttctgcggct gcgttctggt tcatgaccag cttgtaaatc
    23821 tctaactgga atttctgttc ttcagtcatg ggaataatct ctgccattgt tggctccgtt
    23881 tatccgttaa aagggatatc agttaagtta tcccgtgtag ggtataagcc attgtcgaga
    23941 ccactcattg aatggcctct gcaataaccg atgtctttcc atcagtccgc caccacaaag
    24001 aatctttttt gccataaggc tggaggttca tctttcagtg gctgccagtg ttatttcccc
    24061 acttactggc ttgggttgtt tcgctgtact gccgttaatt ggtgagtccg gggattattt
    24121 cagttcgtta ccaggcattt cttttagctc tttcaaatga caacgattga gactaaacca
    24181 ctccccgtgc gacctatagt tgtaatattt ttggtgcaat ttggtttcaa gctctctatc
    24241 ggccggaatc tttgcaatta gatttagctt cccaccactc atgcgagata tctctgaaat
    24301 ccgtttatta accctgcgac taaaccctat ttttgttagc ccactatctt cagcatgcag
    24361 aacgtacaca tatgattttt gcgaagcact gggtgcgact ttgttgtaat taatcatgtc
    24421 aaacatgaag ccttgcttta gcaaagtttc aaaaaagata gaattaacac acccattcct
    24481 tctcagctct gcacttagct tgtcaatttc ctctatgatg tcgccagacc cctttccact
    24541 tataagaaaa tcttggtaca tgcgaccaat tcttgaggta atttcaacaa agttattcat
    24601 agcgtttacc ttttagaaag atgagcctgt tcgcacagaa aagccgtccc cgagatggtc
    24661 gccaccatat acggcaattc tcaggctcag ctttctgaaa gactcgggat tgttacgcgc
    24721 tgcgatgcgc ggtttactgc agatgtaaaa aagccccgca aatgcgaggc taaatcctgg
    24781 tatttgtaat gactggctct tatctcaacg cagcccctta ccgcgcgcca gatgctcaat
    24841 atcaagcatc agcaatgaga tgtttaatct ggattcactc cagaagtgat catcaccctg
    24901 tctacagagc tagatgtgaa ggatgatgag taaaattatc gctatcatcg aaggcattgc
    24961 gtcctgatgt attcctgcag gtagttaacc tgcgcggtta tcctgtcgat tccacttctg
    25021 agacggtaat aattgagttc agcatctgct gtaagtcttg ggctttctcc atcgcccatg
    25081 ctgctggctc cggtcgttga ctttgcacag gtggcggcga cttgcaggcg cttacgccca
    25141 gcagaaacat cagcacggag gctttcgata gtcgcgttag catcagcaag ctcctttgtg
    25201 tatctggcgt caagttctgc tacatcacgt tgacgcttct gcatgtcagc gattgtggat
    25261 gtggccttat cgcgctgctc tttgtaggtc atggcgttat cacggtaatg attaacagcc
    25321 catgacaggc tgacgatgat gcagataacc agagcggaga taatcgcggt gactctgctc
    25381 atacctcaat ctctctgacc gttccgccag cttctttgaa ttttgcaatc aggctgtcaa
    25441 ccttatgctc gaactgacca taaccagcgc caggcagtga agcccagata ttgctgcaac
    25501 ggtcgatagc ctgacgaata tcaccgcgat caatcatcgg taaagcgcca cgctctttaa
    25561 tctgttgcaa tgccacagcg tcctggcttt tcggagagaa atctttcagg ccaaactgct
    25621 tacgataggc atcccaccaa cgggaaagaa gctggtaacg gcctgcagct gttgatttga
    25681 gtttggggtt tagcgtgaca agtttgcgag ggtgatctga gtaatcagtg aatagctctc
    25741 cgcctacaat gacgtcataa ccatgatttc tggttttctg acgtccgtta tcagtcccct
    25801 ctgaccacgc cagcatatcg aggaacgcct tacgttgatt attgatctcc accatcttct
    25861 actccggctt ttttagcagc gaagcgtttg ataagcgaac caatcgagtc agtaccgatg
    25921 tagccgatga acacgctcgt tatataagcg agattgctac ttagtccggc gaagtcgaga
    25981 aggtcacgaa tgaaccaggc gataatggcg cacatcgttg cgtcgattac tgtttttgta
    26041 aacgcaccgc cattatatct gccgcgaagg tacgccattg caaacgcaag gattgccccg
    26101 atgccttgtt cctttgccgc gagaatggcg gctaacaggt catgtttttc tggcatcttc
    26161 atgtcttacc cccaataagg ggatttgctc tatttaatta ggaataaggt cgattactga
    26221 tagaacaaat ccaggctact gtgtttagta atcagatttg ttcgtgaccg atatgcacgg
    26281 gcaaaacggc aggaggttgt tagcgcaacc tcctgtcacc agctttcacg aagccagaca
    26341 ttgagctagt tttcttttat gcaaagcaca ccgcaccgta gccacagcgg ataaggtgat
    26401 tatttttgtc tgtctggtat ttggtttgat gtgctttcag aaaggccgtg cttaaaacac
    26461 aaaaagcccc gagctattaa ctcagggctt tatttaacga gtgcatttat ccatcgttga
    26521 tgtcaaattt acccaacttt attcaaaaag tcaatatcat gctgttaata tgttgccatc
    26581 cgtggcaatc atgcagctaa cgtgtgaccg tattcaaaat gttgtctgcg attgactctt
    26641 ccttgtggca ttgcaccacc agagcgtcat acagcggctt aacagtgcgt gaccaggtgg
    26701 gttgagtaag gtttgggatt agcatcgtca cagcgcgata tgctgcgctt gctggcatcc
    26761 ttgaatagcc gacgcctttg catcttccgc actctttctc aacaactctc ccccactgct
    26821 ctgttttggc tatatcaacc gcacggcctg taccgtggca atctctgcat cttgcgcccg
    26881 gcgtcgcggc actacggcaa taatccgcat aagcgaatgt tgcgagaact tgcagtacct
    26941 ttgccttagt atttccttca agctttgcca caccacggta tttccccgat accttgtgtg
    27001 caaattgcat cagatagttg atagcctttt gtttgtcgtt ctggctgagt tcatgcttac
    27061 cgcagaatgc agccattccg aatccggctt gtgattgcgc catccccata gcagccatca
    27121 catcagtacc ggaaagagag tcagaagccg tggcccgtgg tgagtcgctc atcatcgggc
    27181 tttttggcga atgaaattta gctacgcttt cgagtctcat cgtcttcccc tcttgccctg
    27241 tttgaccatc aggacgccgt taactattac atgacgctcg cctttgctgt ctcggttgta
    27301 cttgagcact gttcctcttg cgcaggaaag catccttgcc acttcagtct gattgcctcg
    27361 tgtctggata agaagctctg gtatcgtttg aattgtggcg ttcatgcgtt ctccagttcg
    27421 gtgattttta ttccaagcct tccgcctggt actttcacgc cacgaattac gcgaatgtca
    27481 tcgaattgct cgtcgtcttc cgcaaatccg gcgtggataa gggagtcgag taaacctttc
    27541 aggatgttgt cgaggtcgcg gcggcgggag tctggaacgt ctgcgatgac tttgatgcgg
    27601 agtcgtgatt tggtgaaaat gtctaactta agttggcgga tgatttgctg aacgtctttt
    27661 cggtatttct ggcctttatc gctgatgtag tattggcttc cccgtcttcg ccagtaggtg
    27721 ttcaccgacg gcgggtatgg aagcacaaac tgatattcgc tcatgactta atcttcccct
    27781 ccttcagcag tatcgactgc gtcctgatca cgccttcgag gtggtaaagc ctggcgtctt
    27841 tgttgtcgag attatgggtg cgtcggtcga tttcatcgtg acacgcgcta caagcccatg
    27901 cgccgatcag gtcgtcaggc ttcattccag ttccgcaaat tccagccatc cggtaatgtg
    27961 ccagaactgt ggtttcagga ttaccattgc atacgccgta aatacgtacc tggcattctc
    28021 tgccgcgcgc ttctttgcgt aggttagcca tttaccttcc ctcgcaattg aagaattgac
    28081 tgaaggtctt ttttaataaa tatgcgagtg cgaattgagc agtagttttc cttcattctg
    28141 gcgtagtaat agtcttttct ttgcttaagc ttgttggcat ccgctgccat ccagtctttt
    28201 acagcaaact taattaacca gcggtggcag agataccatt tcaggtaatc actcatcgtc
    28261 ttcttcctcg tacattgagc tattcggatc gctcatcagt tctgcgcagc aatcggagca
    28321 cacgtgaact tccagcacat gcagcttctg accgcagtta gcgcacgtta aagcccgctc
    28381 gacgctttct ttctggtatt gaagggattg ggatgggcta agcatggctt tcaccattaa
    28441 aaagtcgctt gtaagcatca atgtctcgtt ttgcttcacc gagctttcgt cttaattcca
    28501 tgttttctga ttcaagcttt tccatgtctt gttggtatcg atcgcggtgt tctttccatg
    28561 cttttcgata cgcctttatg tatgtcgttt tggcctttct ctttgcctga cgaacagcgt
    28621 gatggttttc tacaaaccag tcagggtcgt taaatgctgc tctggcgcat gtataccaat
    28681 aatttgttgc ctccctgttt agccaataaa tactgataaa tggcaaccgg atagacacca
    28741 tttttcgttg tgactctttc tcgccaaaca tgtggccttt tttgatgcta agtccaaatc
    28801 caggttgaat taaaagcatt gtcatttcct cgcacgatgt cttagccacc ggatatccca
    28861 caggtgagcc gtgtaattga atgttttcac atcagattct tttgggattg gcttgggttt
    28921 atttctggag cgtttggttg gaaggtattt gcagttttcg cagatgatgt cgtgatactt
    28981 cgtcgctgtc gcctcatgcc gccctcctga cgccatgccc gatcgccatc aatgccgctt
    29041 tggatacggt agtaaacatt cgtcgaggac tgatgaacgg tcgccaaatc agcagcatgg
    29101 agcctttgct gtttcccttc ttctccagcc ctgtcgatgg ctcgataaaa ttaatccgtc
    29161 catcagtgat aatgcgaact tcgtcgacac tctccagagc cttgctgaac catccgactg
    29221 acatatcctc tggcacaagc at
//
LOCUS       DAZFZC010000063        29069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFZC010000063 DAZFZC010000000
VERSION     DAZFZC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.063"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            118..1701
                     /gene="yegH"
                     /locus_tag="ACHKXP_003096"
     CDS             118..1701
                     /gene="yegH"
                     /locus_tag="ACHKXP_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7648050.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1994..3847)
                     /gene="asmA"
                     /locus_tag="ACHKXP_003097"
     CDS             complement(1994..3847)
                     /gene="asmA"
                     /locus_tag="ACHKXP_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO7648051.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQVSNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3869..4450)
                     /gene="dcd"
                     /locus_tag="ACHKXP_003098"
     CDS             complement(3869..4450)
                     /gene="dcd"
                     /locus_tag="ACHKXP_003098"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO7648052.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4542..5183)
                     /gene="udk"
                     /locus_tag="ACHKXP_003099"
     CDS             complement(4542..5183)
                     /gene="udk"
                     /locus_tag="ACHKXP_003099"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO7648053.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5501..8818
                     /gene="dgcE"
                     /locus_tag="ACHKXP_003100"
     CDS             5501..8818
                     /gene="dgcE"
                     /locus_tag="ACHKXP_003100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7648054.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETHFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKALQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYCITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISISLPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8927..9775)
                     /gene="alkA"
                     /locus_tag="ACHKXP_003101"
     CDS             complement(8927..9775)
                     /gene="alkA"
                     /locus_tag="ACHKXP_003101"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO7648055.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9909..11261
                     /gene="yegD"
                     /locus_tag="ACHKXP_003102"
     CDS             9909..11261
                     /gene="yegD"
                     /locus_tag="ACHKXP_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7648056.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11273..13213)
                     /gene="yegI"
                     /locus_tag="ACHKXP_003103"
     CDS             complement(11273..13213)
                     /gene="yegI"
                     /locus_tag="ACHKXP_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO7648057.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13210..13971)
                     /gene="pphC"
                     /locus_tag="ACHKXP_003104"
     CDS             complement(13210..13971)
                     /gene="pphC"
                     /locus_tag="ACHKXP_003104"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO7648058.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13968..14627)
                     /gene="yegL"
                     /locus_tag="ACHKXP_003105"
     CDS             complement(13968..14627)
                     /gene="yegL"
                     /locus_tag="ACHKXP_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7648059.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSSSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14848..14907)
                     /gene="ibsA"
                     /locus_tag="ACHKXP_003106"
     CDS             complement(14848..14907)
                     /gene="ibsA"
                     /locus_tag="ACHKXP_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO7648060.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15180..15236)
                     /locus_tag="ACHKXP_003107"
     CDS             complement(15180..15236)
                     /locus_tag="ACHKXP_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7648061.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15508..15564)
                     /locus_tag="ACHKXP_003108"
     CDS             complement(15508..15564)
                     /locus_tag="ACHKXP_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7648062.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15843..17090
                     /gene="mdtA"
                     /locus_tag="ACHKXP_003109"
     CDS             15843..17090
                     /gene="mdtA"
                     /locus_tag="ACHKXP_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO7648063.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPSAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAADRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17090..20212
                     /gene="mdtB"
                     /locus_tag="ACHKXP_003110"
     CDS             17090..20212
                     /gene="mdtB"
                     /locus_tag="ACHKXP_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO7648064.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20213..23290
                     /gene="mdtC"
                     /locus_tag="ACHKXP_003111"
     CDS             20213..23290
                     /gene="mdtC"
                     /locus_tag="ACHKXP_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO7648065.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23291..24706
                     /gene="mdtD"
                     /locus_tag="ACHKXP_003112"
     CDS             23291..24706
                     /gene="mdtD"
                     /locus_tag="ACHKXP_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648066.1"
                     /translation="MTNLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHCIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPLVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24703..26106
                     /gene="baeS"
                     /locus_tag="ACHKXP_003113"
     CDS             24703..26106
                     /gene="baeS"
                     /locus_tag="ACHKXP_003113"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO7648067.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLIQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26103..26825
                     /gene="baeR"
                     /locus_tag="ACHKXP_003114"
     CDS             26103..26825
                     /gene="baeR"
                     /locus_tag="ACHKXP_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO7648068.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27016..27348
                     /gene="yegP"
                     /locus_tag="ACHKXP_003115"
     CDS             27016..27348
                     /gene="yegP"
                     /locus_tag="ACHKXP_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO7648069.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27495..28856
                     /gene="yegQ"
                     /locus_tag="ACHKXP_003116"
     CDS             27495..28856
                     /gene="yegQ"
                     /locus_tag="ACHKXP_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO7648070.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
BASE COUNT         6645 a         7805 c         7748 g         6871 t
ORIGIN      
        1 ggttattacc ctcaaataat atgagataaa tagtgctgca acattgcatt tttgccccga
       61 tttatccatg atcgaattgt gacatttgtc atacaacgaa taggttttgt acttactatg
      121 gaatggattg ccgatccgtc tatctgggcc gggttaatca cgctgattgt gatcgaactg
      181 gtcctcggca ttgataacct ggtctttatt gccatcctcg ccgaaaaact accgccgaag
      241 cagcgtgacc gcgcacgggt taccgggctg ctgctggcaa tgttaatgcg cctgttactg
      301 ctggcgtcaa tctcctggct ggtcaccctg actcaaccgc tgttcagctt ccgctcgttt
      361 acctttagcg cccgcgactt aatcatgctg tttggtggtt tcttcctgtt gttcaaagcc
      421 acgatggagc tgaacgaacg gctggaaggg aaagacagca ataatcccac acaacgcaaa
      481 ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac tggacgccat tttctcactc
      541 gactcagtga ttaccgccgt cgggatggtc gaccatttac tggtcatgat ggccgccgtg
      601 gttatcgcta tcagcctgat gttgatggcc agcaagccgt taacgcaatt tgttaacagt
      661 cacccgacga tcgttattct ctgcttaagc ttcctgttga tgattggctt tagcctggtg
      721 gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt acgctgccat tggtttctcg
      781 gtgatgatcg aggcgctcaa tcagttggct atctttaacc ggcgacgttt tctttccgct
      841 aaccagacgc tgcgccagcg gactaccgaa gcggtaatgc gcctgcttag cgggcaaaaa
      901 gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg tggatcatgg taaccagcag
      961 atctttaatc cgcaggaacg gcggatgatt gagcgggtac taaatcttaa ccagcgtacc
     1021 gtcagcagca ttatgacgtc gcgccacgat attgagcata tcgatctcaa cgcgccggaa
     1081 gacgagatcc gccagttgct ggagcgaaat cagcatacgc ggctggtggt taccgacggt
     1141 gatgacgcag aagatttgct cggtgttgtt cacgttatcg acctgctaca acagtctctg
     1201 cgcggcgaac cgctcaacct gcgggtgttg attcgccagc cgctggtgtt cccggaaacc
     1261 ttgccgttgt tacctgccct ggagcagttc cgtaatgccc gcacgcactt tgcttttgtg
     1321 gtggatgagt ttggctcggt ggaagggatt gtgacattaa gtgacgtcac tgaaaccatt
     1381 gccggtaact taccgaacga agtggaagag atcgacgccc gccatgatat tcagaagaat
     1441 gcggacggtt cctggacggc gaatggtcat atgccgctgg aggatctggt gcaatatgtg
     1501 ccgctgccgc tggatgaaaa acgtgaatat cacaccattg ccgggctgtt gatggagtat
     1561 ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg gggattattt gcttaaaacg
     1621 ttgcaggtag aaagccatcg cgtgcagaag gtgcagatta taccgctgcg taaggatggc
     1681 gagatggagt acgaggtgtg atggtatcgg tcattttacc cgatgctgcg taacgcctta
     1741 taacttcagc aatgaagcgt tgtctgtggt acatggacat cacagcgggg gtgtgggccc
     1801 gggttgccag ggtggtggcg attgagccac cctggcacgt tcaccggctt gagcgttcca
     1861 tagtaacgag gaacatcaag tgaacggaac agccttcgat cttacatatt cagaatataa
     1921 tgatgccgcc acgaacgcct gatgcgacgc tttacgtgtc ttatcaggcc tacaggactg
     1981 gtcacctggt aacctacatc ttctcaagca acttcttcac atctttgcca ttacgggaat
     2041 ctttattccg ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt agatgtttgc
     2101 gcagtaactg atccacttgc aggctgtaat tgagttgctg ccagttgcca taaacccgca
     2161 gcggtactgg cgtttctttc aggaaatcaa tcagtttgct ttccccgttc cagccaccca
     2221 cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc cagattcaac atgccttccc
     2281 ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt taacgtcacg acgccatcct
     2341 tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac gttatcaaag ttttcagcgg
     2401 ccttcacatc accgccatta cgctctaccg cttgctgaat catctgctgg aagttcatcc
     2461 cttccatgcg cgtgtcggtc atttcgacat gtgcctgtcc ttgccagttg tggcggaatg
     2521 cgtcggcatc tatgtcagca ccggagaaat caccagccag tgacattttt ccggtcaacg
     2581 aaatcggata gttaaacgcc ttcagaatgg taccaatctc aacgttttcc agccgtggct
     2641 ggaagtttat ccgcggattt attgatgtcg cgtccagcgt gcccggcagt gaaacctgtc
     2701 caccgttaag cttgccctgc agttgagtaa tttccagcaa acccgacttg ttggtcattt
     2761 gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac gttactgacc tgcaacaaaa
     2821 tatcagccgt aaagccttgc agtccctgat aggccggttc atcaatacgc gaagaaataa
     2881 ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc ggcaccgttt tcaccattcg
     2941 ctgtttcatt aagcgggatg aggttgtcaa gattcagttg cgggaattgc agcctcagct
     3001 gccattccgg tttctctgtc agcgtaacct gtgcttgccc gctcagcgta ctgtcattgg
     3061 cggtcaagct gatttggtta aatgaaaggc gtttatgtga ctcctgccac tgggcctgga
     3121 aactcccctg cccctgaata ccttgttttg gtaaatcggc accctgcaac tgccagttaa
     3181 tttgttcaat agccgccgtt aaatcatgcg gataatcaga agcatctacc gtaccgttaa
     3241 gggatatcgt gagatcgcgc tgatcgcgat taacccgccc ggaaaactca aatgagccac
     3301 gatgttgggg atcttgttcc atttgcaggc ggatattgcg gattgtcacc tgctcgtcat
     3361 cttcatgctg gaacaccaga acgctgtccg ccaccttaag actggatata tcaaacgacc
     3421 atccgcgatc gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc ggagcgtctt
     3481 cactgcgcac cgcttccgtc tgcggcgtca gttggatcac tgcccctttt agcatcacct
     3541 gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga cgcatgttgt
     3601 cggcgcgaac cagtggctgg cttgcgccct gggcggtgag agacattcgc ccggagagga
     3661 tactaagctg cggccagacg tgccaacgca gtggcccgtc gagctgcaat tgataaccgc
     3721 tacgtgcagc aacttgcttg accatatagt cgcggaaatc attcggattc accagcaaca
     3781 ctaacgcaga taacccggcc accagcacga ccaggagtat catcagcgtc gtcagaaatc
     3841 gtctcatggt atcctcaatg ggcctgaatt agtctttatc gattcggctg gctaccgcgc
     3901 cctgctggtt gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc gccgggccgg
     3961 aaagcggctc aaagctcagc gcaccaatta acatgcccgg acgcagcgcc agcggcagct
     4021 taccggagtt gtagaactct agcacaatgc aaccagacca gcccggatcg atgcggtgcg
     4081 cggtgacgtg caccatcagc cccagacgcg ccagtgagga acgcccgtcc agccagccca
     4141 ccagatcggc tggcagcgtc accgactcca gcgtcaccgc cagcgccagc tctcctgggt
     4201 gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc gctcatcacg cggtcaagcg
     4261 cggcgctcac ttcatctttg ggaccgctca aatcgataaa cgctgccgtg tgaccacgga
     4321 aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc gccgttaata cgctccactg
     4381 gcggacgtgg gttgatcgac aaacggcctt catcaagcca ggcttcaata tctcggtcac
     4441 acagacgcat ggcattttct cctttcgcgc atcactccct taacgccaat tacgtcaagg
     4501 gcataccagg ttatcactga acggtacaca atttatcaag cttattcaaa gaactgactt
     4561 attttcgctt tcaatatatc gatcgcgatg cggtttttcc cgccgcgcgg cacgataatg
     4621 tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca tcgggcgcac ggttttttga
     4681 tattgcgcca tcactgaatc cattgaacgc ccacgctcgt taacgtcacg cttgatgcgg
     4741 cgcatcaggc agatatccag cggggtatca acgaaaatgg agaagttaag ttcgtcacgc
     4801 aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga tgaccttctt cggctcaacc
     4861 gtcaccgttt ctttcatacg cgtatgttca acatagctgt aaaccggcag gtcaattgcc
     4921 gagccgcgtt tcaacgcttg taaatgctca agcagcagac tgtgatccat cgcgctggga
     4981 tggtcgtagt tggtcttaac gcgttcttcc atcgacagat ggctttgatc tttgtaatag
     5041 cagtcttcgg gaattacgcc gatgtgttca tcaccgactt gctcacgcaa ttcacgataa
     5101 agggtactgg caataagact cttgccggaa gccgatgcgc cagcgatacc gataatgacg
     5161 cactgatgag actgatcagt catatattta gcgacctgat taacctggat gttaggaagg
     5221 gggcgacgaa gcgccaaacg cggcaattat agggatttca tccgtctgat accagtcgaa
     5281 tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa ttcccggggg caaattacgt
     5341 tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt gcgcaaatta tccgccttta
     5401 cgtcacttta tgagcaattc gcatataaaa tgtaaaactt ttgtactagc ataaacacag
     5461 aaacgaatac tggcgaccag gtcttgcgga taaagcggta atgagcaaac aatcacagca
     5521 tgtattaatt gccctgcccc acccgctgct tcacctggtc agtttaggtt tagtctcgtt
     5581 tatctttacc cttttctcgc ttgagctttc gcagtttggc acccaactcg ccccactgtg
     5641 gttcccgacg tccatcatga tggtggcgtt ttatcgccat gccgggcgca tgtggccggg
     5701 aattgcgctg agctgttcgc tgggaaatat cgccgcatcc atcctgcttt tttccaccag
     5761 ctcgctgaac atgacctgga cgaccatcaa tattgttgaa gccgtggtcg gggcagtgct
     5821 gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat ctggctgact ggctgcgtct
     5881 ggcactcggc agcgccattg ttccgcctct gttggggggt gttctggttg tcctgctgac
     5941 gcccggagac gatcctctca gggcattttt gatatgggta ctgtcagaat ccatcggcgc
     6001 tctggcactg gtgccgctgg gattgttatt taaaccacac tatctgctgc gccatcgcaa
     6061 cccacggttg ctttttgagt cgctgctcac gttagccatc acactgacgt taagctggct
     6121 ttcgatgctg tatctgccgt ggccttttac tttcattatt gtgctgttga tgtggagcgc
     6181 cgtgcgtctg ccacgaatgg aagccttttt gatcttcctt accacggtga tgatggtgtc
     6241 gctgatgatg gccgcggatc cctccctgct tgctacgccg cgtacgtacc tgatgagcca
     6301 tatgccgtgg ctaccgtttt tgctgatcct gctgcccgcc aacatcatga ccatggtgat
     6361 gtatgccttt cgtgcggaac gcaaacacat ttccgaaagc gaaacccact ttcggaacgc
     6421 gatggaatat tccgctatcg gtatggcgtt agtgggcacc gagggacaat ggctgcaaac
     6481 caacaaagcg ctctgccagt ttctcgggta cagtcaggaa gagctgcgcg gactcacctt
     6541 tcagcaactg acctggccgg aggatctcaa taaagctctc caacaggttg aaaagctgat
     6601 aagcggtgaa ataaacacct attcaatgga aaaacgctac tacaaccgca atggcgatgt
     6661 tgtctgggcg ttgcttgccg tctcactggt gcgccacacg gatggcacgc cgctctattt
     6721 tatcgctcag attgaagaca ttaacgagct aaaacgcacc gaacaggtca atcagcaact
     6781 gatggagcgc atcacgctgg ctaacgaagc gggcgggatt ggcatctggg agtgggagtt
     6841 gaagccgaat atttttagct gggataagcg gatgttcgag ctgtatgaaa ttcctccgca
     6901 tatcaaaccg aactggcagg tgtggtacga gtgtgtattg ccggaagatc gccaacacgc
     6961 cgaaaaagtg attcgtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat
     7021 tgccgtgaaa gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga
     7081 aggcgaagtc gaacgcctgc tcggcattaa tatggatatg actgaggtta aacagcttaa
     7141 cgaggcattg tttcaggaaa aagagcgcct gcacattacg cttgattcca tcggtgaagc
     7201 cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagtgg cggagaagat
     7261 gagcggctgg acgcaggaag aagcgttagg tgttccgctc ctgacggtgt tgcatattac
     7321 ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc
     7381 gatcgagcaa gatgtggtgt tgcactgtcg gagcggcggc agttacgacg tgcattactg
     7441 tattacgccg ttaagtactc tggacggcag caatattggt tcggttctgg tgattcagga
     7501 cgttaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact
     7561 gacgcatctc gccaaccgcg ccagttttga gaaacaactg cgtatcctgc tgcaaacggt
     7621 aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt
     7681 gaatgacagc gccgggcatg cggcgggtga cgctttgctg cgcgaactgg cgtcgttaat
     7741 gctgagtatg ctgcgttcca gcgacgtgct ggcgcgactc ggtggggatg aatttggtct
     7801 gctgctacca gactgtaatg ttgaaagcgc gcgttttatc gctacacgca ttatcagtgc
     7861 cgtgaatgac tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg
     7921 gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat
     7981 cgcctgttat gcctccaaaa atggtggccg gggccgggtg acggtttacg aaccgcagca
     8041 agctgccgca catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa
     8101 agagaatcag ttgatgatgc tcgcccacgg tgtcgcttcg ccacggatcc cggaagcgcg
     8161 taatttgtgg ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca
     8221 aacatttcgt cgtagcttca gcgatccggc gcttagccat gctcttgacc gccgggtatt
     8281 ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt aaaggcataa gcatctccct
     8341 ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct
     8401 ggaaaatagc cctctaccac cacggttatt acatctgatt attccggctg aagcgatttt
     8461 agatcacgca gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag tgctcagcca
     8521 ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct
     8581 acttgatggt gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac
     8641 gattattcag gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt
     8701 acccttagtg atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgaggtgat
     8761 tgccgatgcc caaccgctgg atttgctggt gaatagtagt tatttcgcga ttaactgagg
     8821 cgtgcttccc catcgcctga tgcgacgcta acgcgtctta tcatgcctac aaatcgctca
     8881 ttccccaggc cggataaggc gctcgcaccg catccggcga ccaacgtcat gcttcgtctg
     8941 gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag ggcttccagc
     9001 gctcggcata acggcggatt tgtgccggtg tcatccccgg aaatcgctgt ttaatcagat
     9061 aatcatccgg cagaaaaaca tctttcgcct gccagccacg caaagcgaaa taattcgccg
     9121 tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc acatcgcccg
     9181 gaattgtcat tggtaagctg ccctccagcg ccgcatttgc cagatgaatc agtgcctctg
     9241 cccgtttcaa cggcatgcct aacgctttta atgcctgcgg gtcggctgct gccagccgct
     9301 gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca taaagctgta
     9361 caactttggc agtcaatttt gccgccatcg ccacgctcac cagttggcct aaaatcgccc
     9421 gcacaccctg ctcaaaagca tcaatacagc cgggtaaacg caatccgggc cgcgccgcgc
     9481 ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca aacaggcggc
     9541 tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa tttatgtgca
     9601 gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg cccaccgcca
     9661 gactacgggc ataataatcc tcagcgaccg tttccacacc actcaccgca cgggcggcga
     9721 gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcagggta tacatcgcat
     9781 ctccttattc atccgctttc agcataaacg ttattcagac gctacgcttt gctttcatat
     9841 tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac cggcatgggg
     9901 attaattcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc
     9961 gtgacggtaa accgcaatta ctgaaaatgg aaaacgacag cacgctgctg ccttcaatgc
    10021 tttgcgcgcc aacgcgtgaa gcagtaagtg aatggctgta ccgccatcat gatgttccgg
    10081 cagacgacga tgaaacgcag gcgctgttac gtcgggcgat tcgttataac cgcgaagaag
    10141 atatcgatgt tacggcgaaa agcgtgcagt tcggtctttc ctcactggca cagtacattg
    10201 atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt gccagcggct
    10261 taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg ttgcacattc
    10321 gccagcaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt ggtcgcccga
    10381 tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcaaggg attctggaac
    10441 gcgcggcgaa gcgtgccgga ttcagggacg tggtattcca gtacgagccg gtcgcggctg
    10501 ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg gatatcggcg
    10561 gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg cgtctcgatc
    10621 gtgaagccag cctgctgggt cacagtggtt gccgtattgg cggtaacgat ctggacatcg
    10681 cactggcgtt taaaaacctg atgccgttac tgggcatggg tggcgaaacc gaaaaaggca
    10741 tcgccctgcc gattctgccg tggtggaatg cggttgccat caacgacgta cctgcgcaga
    10801 gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc gatgcccgcg
    10861 aaccggaaaa agtggccctg ctacagaaag tctggcgtca gcgtttaagc tatcgcctgg
    10921 tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc cgcgcctcac
    10981 tgccgtttat cagcgatgaa ctggctacgc tgattagcca gcaagggctg gaaagcgccc
    11041 tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat aacgcccagg
    11101 aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttcgccgctg attaaaaaag
    11161 cgctggcaga acagttgccg ggaattccga ttgcaggcgg cgatgacttt ggctccgtca
    11221 ccgccgggct ggcacgctgg gcggaagtgg tgtttcgtta aaagaaaatg gctcagcagc
    11281 ggcttaaatc tgcctgcgcc tgagccagtt tttctgccgc ctgacgtaaa ggttccatca
    11341 acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc gcgccgacag
    11401 tcaacgtcga ttccagccga tgtcgtttgg cggtcatttt cgccattacc gcttgtctgt
    11461 ctgctggcgt tatcgcttca ttcggcctga acacaaaacg gcgttcacag ctcgctttcc
    11521 agtcgataac cgcctgggtc agatggtcgc caaacccttt cacttgctta acgccacgac
    11581 gggtaacatc tgcggctgtt tcaataccaa aagaccgtaa cgccgcttta cgcgcagggc
    11641 caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc tgcttctgcc
    11701 gttcccttgc agtgtcatga agtgctgcca gagcgcgttt ctcttcttcc ggtaacccga
    11761 gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct tccaggccgc
    11821 ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga ttaaatgcct
    11881 cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca ataatccaga
    11941 tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg ccgcaaagca
    12001 gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc cgtaacaggc
    12061 ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt tgcaatgctg
    12121 gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa tccccgccgg
    12181 tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta tccagcgcac
    12241 accacgggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg ggacatttct
    12301 ttaactgttg gcgcagagaa tcaagtgccg ctacccacgc tttagccgtc ggacgcccgg
    12361 ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca ctcggtaaca
    12421 tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta tctgacgcgt
    12481 aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca gagataagcg
    12541 gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc aacgcaaggc
    12601 caaaattatc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag gtttgtagct
    12661 ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca ttggcgttaa
    12721 tctgaaagga gtcgctatca atcagtacca ctttgctgtc gcgacctacc ataaaactgt
    12781 tctggtttac gtcacccacg acgtgcccgt gctcgtgaac cgtagcaaaa gatgaagcaa
    12841 tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt tgacgacgat
    12901 gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc atcataaaac
    12961 cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg acataattca
    13021 acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt aaggcgggtg
    13081 gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca taaaccgcgc
    13141 cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcaccagta gcagtatata
    13201 aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat ccgtacgctc
    13261 gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat cgagttgttc
    13321 ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggtacat gaggagaatt
    13381 atccagcata tttaacgcca gtcgctggat accatccgta aatgctgcaa ctttatgcac
    13441 acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg taatgaagtg
    13501 cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac cgtgaccaaa
    13561 atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag ccgatgaaat
    13621 caatcccaga aacgtgcagg caaaatcgcg aactgctaat tctttggctt ccgcctcggc
    13681 aaaaagtcgc tgacgaatag tcagcacaat atccgtggcg aggatatcat taagccccaa
    13741 ttccccaccc tgcacttttt gcgacatata agccatcgct tcattgacgg cgagcatcgc
    13801 gccttcgcca ccctgcgaga cgctgcctgc accatcagca agaaacatca ccagtaatgg
    13861 ttgctgatcg tttaaccagg caacctgcat ctggcaggca tcctgacaag gtaaatcagc
    13921 gctaatgtgc gacgttccaa cagcggatgc ataaaccaga cgccagctca cactgacgtc
    13981 cagccttttg gcgcttccag cacaacttcc gttcccggcg tggagcggga aaccgaacgc
    14041 agcgaactgg ataaccagct aaacagttca cggaattgta atccttgcag aggcaaaggc
    14101 tgacgaacgc tgatttgtgc cagcgtcttc atatccgccc cctgaacagc gatggaaaag
    14161 aaggcaaact tcttatcttc ttccccctga aacactttgt tggcggcagc ctgccactca
    14221 tcggttggag cgccatcggt gatcaggaaa atccacgggc gataatacga aataccattt
    14281 gcgcggtact cacgtttcct ctcttcaacc atatccaggg ctttggtaat ggcggccccc
    14341 attggcgtat ccccctgggc aaacaggatg ggcgggaaaa agttagcggc gctggtaaag
    14401 ggttgttcca catgcaccgg gccgaacgtc acaataccga gttccactct ttttaaggcc
    14461 agcgaatcgg caagcagctc atcgcgaaag gtaaccaatc cggcgttaag ttcattaatg
    14521 ggtctgccgc tcatcgagct agaaacatcc agcagcaaaa tgcaggggca acgtggttcc
    14581 gggttactgg caaaatcgct ggtggcaaac gtgatttgtt cgctcataag aaatcctttc
    14641 ttagagaatt attgaggtcc ggtatcatac cgactgcaat aatgaaataa gagcaaacga
    14701 agaaactttc agaattaggc ccggcggcgc agaaaatttt gctattcgcc tcgcaagaac
    14761 ggagagccgt cgattgacac taagggcgga gtgacataat ttcaggagtg agggttaggg
    14821 agaggtttcc ccctccccct ggtgttctta gtaagcctgg aagctaatca ctaagagtat
    14881 caccagtatg atgacgtgct tcatcataac cctttcctta ttaaaagccc tcttctccgg
    14941 gagaggcttt cccgtttcag cgtcccgctg aaatcatcgg cttacctcct ttcgccctga
    15001 atgcagtcta tcgctaacgc gtgaataaaa tagtttcctc tgtgattatt gcgtaacgac
    15061 tcgcaaagaa tatttgctgg caggatcgca gactacaaag cctgcggatt gacaatctta
    15121 tcgtgaaggc atactttcag gagtgagggt agagcggggt ttcccccgcc ctggtagtct
    15181 tagtaagcgg ggaagcttat gactaagagc accacgatga tgagtagctt catcatgacc
    15241 ctttccttat ttaaggcccc ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa
    15301 atcgtcggct tacctccttt cgccatgcac ttagtctatc gctgacttgc agataaaata
    15361 gtttccagtg tgattattgc gtaacgtctc gcaaagaatt tttgctggca ggatcgcaga
    15421 cggcaaatcc tgcggattga caatcttatc gtgaaggcat actttcagga gtgagggtag
    15481 agcggggttt cccccgccct ggtagtctta gtatgctggg tagctaatca ctaagagcac
    15541 cacgatgatg agtagcttca tcatgaccct ttccttattt aaggcccctt cctcgggagg
    15601 ggctttcccg tttcagcatc cggctgaaat ctgcggctta cctcctttcg ccctgtatgc
    15661 agtctatcgc taacgcgtag ataaaatagt ttcctgtgtt attactggat gcgtgctcgc
    15721 aaatgtgccc gccattcaga cgattcccga tagtgtttca taattcctcc atttttctcc
    15781 cttattggct ggctacacta gtatcattcc gcgaaacgtt tcaggaagag aaactcttaa
    15841 cgatgaaagg cagttataaa tcccgttggg taatcgtaat cgtggtggtt atcgccgcca
    15901 tcgccgcatt ctggttctgg caaggccgca atgactcccg gagtgcagcc ccaggggcga
    15961 cgaaacaagc gcagcaatcg ccagcgggtg gtcgccgtgg tatgcgttcc ggcccattag
    16021 ccccggttca ggcggcgacc gccgtagaac aggcagttcc gcgttacctc accgggcttg
    16081 gcaccattac cgccgctaat accgttacgg tgcgcagccg cgtggacggc caactgatag
    16141 cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca gaaattgacc
    16201 ccagccagtt caaagttgca ttagcacaag cccagggcca actggcaaaa gataaagcca
    16261 cgcttgccaa cgcccgccgt gacctggcgc gttatcaaca actggcaaaa accaatctcg
    16321 tttcccgcca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa ggcaccatta
    16381 aggctgatga agcaagcgtt gccagcgcgc agctgcaact cgactggagc cggattaccg
    16441 caccagtcga tggtcgcgtt ggtctcaagc aggttgatgt tggtaaccaa atctccagtg
    16501 gtgataccac cgggatcgtg gtgatcaccc agacgcatcc tatcgattta gtctttaccc
    16561 tgccggaaag cgatatcgct accgtagtgc aggcgcagaa agccggaaaa ccgctggtgg
    16621 tagaagcctg ggatcgcacc aactcgaaga aattaagtga aggcacgctg ttaagtctcg
    16681 ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt aataatcagg
    16741 atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac accgaacaaa
    16801 acgccgtagt gatcccatca gccgccctgc aaatgggcaa tgaaggccat tttgtctggg
    16861 tgctgaatag tgaaaacaag gtcagcaaac atctggtgac gccgggcatt caggacagtc
    16921 agaaagtggt gatccgtgca ggtatttctg cggccgatcg cgtggtgaca gacggcattg
    16981 atcgcctgac cgaaggggcg aaagtggaag tggtggaagc ccagagcgcc accactccag
    17041 aagagaaagc caccagccgc gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt
    17101 acccccgagc agcacaggcg gcccgtctcg cctgtttatt atgcgtcctg tcgccaccac
    17161 gctgctgatg gtggcgatct tactcgccgg gattatcggt tatcgcgccc tgcccgtttc
    17221 ggcgctgccg gaagtggact atccgaccat tcaggtagtc acgctttacc caggtgcaag
    17281 cccggatgtc atgacctctg ccgttaccgc gccgctcgag cgccagttcg ggcagatgtc
    17341 tggcctgaaa cagatgtcgt cgcaaagttc cggcggtgcg tcagttatca ccttacagtt
    17401 ccagctaaca ttaccgctcg atgtcgccga gcaggaagtg caggccgcga ttaacgccgc
    17461 cactaacctg ctaccgagcg atctgcctaa cccgccggtt tacagcaaag tgaacccggc
    17521 agatccgccg atcatgacgc tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga
    17581 agatatggtg gaaacccgcg tcgcgcagaa aatctcgcag atttccggcg tcggcctggt
    17641 gacgctttcc ggcggtcagc gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc
    17701 cgccctcggc ctgaccagcg aaaccgtgcg caccgccatt accggcgcta acgttaactc
    17761 ggcaaaaggt agcctcgacg gcccttcccg tgcggtcacg ctttccgcga acgaccagat
    17821 gcaatccgcc gaagagtatc gccagctaat catcgcctac cagaacggcg cgccaattcg
    17881 tctgggcgat gtcgcaactg tagagcaagg tgcagaaaac agctggctcg gcgcgtgggc
    17941 gaacaaagaa caggccattg tgatgaatgt tcagcgccag cccggtgcta acattatctc
    18001 caccgccgac agcattcggc agatgctgcc acagctcact gagagtctgc cgaaatcggt
    18061 gaaggtgaca gtgctttccg atcgcaccac caatatccgc gcatccgtcg atgatactca
    18121 gtttgaattg atgatggcta tcgcgctggt agtcatgatt atctacctgt ttttgcgcaa
    18181 tattccggcg accatcattc ccggtgttgc tgtaccgctg tcgttaatcg gcacttttgc
    18241 ggttatggtg tttctcgatt tttcaatcaa taacctgaca ctgatggcgt taactatcgc
    18301 caccggattc gtggtcgatg acgccatcgt agtgatcgaa aacatttccc gctatatcga
    18361 aaaaggcgaa aaaccgttgg cggcggcgct caagggcgca ggtgaaatcg gctttaccat
    18421 tatctcgctg accttctcgc tgattgcggt gttgatccca ctgctgttta tgggcgatat
    18481 cgtcgggcga ctgttccgcg aatttgctat taccctggcg gtagcgattt tgatctcagc
    18541 ggtggtgtcg ctgaccctga caccgatgat gtgcgcgcgg atgctcagcc aggagtcgtt
    18601 gcgtaaacag aaccgcttct cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc
    18661 ctatggtcgt ggactggcga aagtgctgaa tcatccgtgg ctgaccttaa gcgtggcact
    18721 cagcacgctg ctgcttagcg tgctgctgtg ggtgttcatt ccgaaaggtt tcttcccggt
    18781 acaggacaat ggcattattc agggcacttt gcaggcaccg caatccagct cctttgccaa
    18841 tatggcccag cgacaacgcc aggtcgcgga cgtgattttg caggatccgg cagtgcaaag
    18901 cctgacctca tttgttggcg ttgatggcac taacccgtcg ctgaacagtg cacgtttaca
    18961 aatcaacctc aaaccgttgg atgaacgtga tgatcgggtg caaaaagtca tcgcccgtct
    19021 gcaaacggcg gtagataaag tgccgggcgt cgatctcttc ctgcaaccaa cgcaggatct
    19081 gactattgat actcaggtca gccgcaccca gtaccagttt accttgcagg ccacgtcact
    19141 ggatgcgctc agtacctggg tgccacagtt gatggaaaaa ctccagcaac tgccacagct
    19201 ttctgatgtc tccagcgact ggcaggacaa agggctggtg gcgtatgtca atgttgatcg
    19261 cgacagcgcc agccgtctgg ggatcagcat ggcggatgtc gataacgccc tgtacaacgc
    19321 gtttggtcag cggctgattt ccactattta tactcaggcc aaccagtatc gcgtggtgct
    19381 ggagcacaac accgaaaata ccccaggcct cgcggcgctg gataccattc gcctgaccag
    19441 cagcgacggc ggcgtggtgc cgctaagctc aattgccaaa attgagcagc gttttgcgcc
    19501 gctctccatc aaccatctgg atcagttccc ggtaacgacc atctccttta acgtgccgga
    19561 taactattcg ctgggcgatg cggtgcaggc gattatggac accgaaaaga cgctgaatct
    19621 gccggtggat atcaccacgc agttccaggg cagcaccctc gccttccagt cggcgctggg
    19681 cagcactgtc tggctgattg tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct
    19741 gtacgagagc tttattcacc cgatcaccat tctctcgacg ctacccaccg caggggttgg
    19801 cgcactgctg gcgttgctga ttgctggtag cgaactggat gtgattgcga ttatcggcat
    19861 tattttgctg atcggtatcg tgaagaagaa cgccatcatg atgatcgact tcgcgctggc
    19921 tgccgagcgc gagcaaggca tgtcgccgcg cgaggcaatc taccaggctt gtctgttgcg
    19981 ttttcgtccg atcctgatga ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt
    20041 gagtaccggg gtcggcgcgg aactgcgtcg tccgttaggt atcggcatgg tcggcggcct
    20101 gattgtcagc caggtgctga cgctgttcac cacgccggtg atttatctgc tgttcgaccg
    20161 cctggcattg tggaccaaaa gccgctttgc ccgtcatgaa gaggaggcgt aagtgaagtt
    20221 ttttgccctc ttcatttacc gtccggtggc gacgatttta ctgtcggttg ccattaccct
    20281 gtgcggcata ctgggcttcc gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc
    20341 ggtgattatg gtcagcgcct cgctgcccgg tgcgtcacca gaaacaatgg cgtcttccgt
    20401 tgccacgccg ctggagcgct cacttgggcg cattgccgga gtcagtgaaa tgacctccag
    20461 cagttcgctc ggcagcacgc gtattatttt gcagtttgat tttgaccggg atatcaacgg
    20521 cgcagcgcgt gatgtgcagg cggcgatcaa cgctgcacaa agtttgctgc ccagtgggat
    20581 gcccagccgc ccgacctatc gcaaagcgaa cccgtcggat gcaccaatta tgatcctcac
    20641 gctgacgtcc gatacttatt cgcagggtga actgtacgat ttcgcctcga cgcagctggc
    20701 tccgacgatt tcgcaaatcg acggtgttgg tgatgtcgat gtcggaggca gctcactgcc
    20761 cgccgtacgc gtcgggctga atccgcaggc gctgtttaat cagggcgtgt cgctggacga
    20821 cgtacgcacc gccgtcagca atgccaacgt gcgtaaaccg cagggcgcgc tggaagatgg
    20881 cactcaccgc tggcagatcc agaccaatga tgagctaaaa accgccgctg aatatcagcc
    20941 gttgattatt cactacaaca acggcggcgc ggttcgtctg ggcgatgtgg cgacggtgac
    21001 cgactcagtg caggatgtgc gcaacgccgg gatgaccaac gccaaaccgg ctattttact
    21061 gatgatccgc aaactgccgg aagccaatat tatccagacg gttgacagca tccgggcaaa
    21121 attaccggag ttgcaggaaa ccattccggc ggcgattgat ctgcaaattg cccaggatcg
    21181 ctcccccacc attcgcgcct cgctggaaga agtcgagcaa acgctgatta tctcggtggc
    21241 gctggtgatt ctggtggtgt ttttattcct gcgctcgggt cgcgccacta ttattcccgc
    21301 cgtttcggtg ccggtttcgc tgattggtac gtttgcggcg atgtacctgt gcggattcag
    21361 tctcaataac ctttcgttaa tggcgctcac catcgctact ggtttcgtgg tggatgacgc
    21421 catcgtggtg ctggaaaaca ttgcacgtca tctggaagcg ggaatgaaac cgttgcaagc
    21481 cgcactgcaa ggtactcgcg aagtcggttt tacggtgctg tcgatgagtc tgtcactggt
    21541 ggcggtgttc ctgccgctgc tgttgatggg cggattgccg ggccgactgt tacgcgaatt
    21601 tgccgtgacg ctttctgtcg ccattggtat atcgttgctg gtttctctga cattaacgcc
    21661 aatgatgtgt ggctggatgc tgaaagccag caagccgcgc gagcaaaagc gactgcgtgg
    21721 ttttggtcgc atgttggtag ccctgcaaca aggctacggc aagtcactga aatgggtgct
    21781 caatcatacc cgtctggtgg gcgtggtgct gcttggcacc attgcgctga atatctggct
    21841 gtatatctcg atcccgaaaa ccttcttccc ggagcaggac actggcgtgt tgatgggcgg
    21901 gattcaggcg gatcagagta tttcgtttca ggcgatgcgc ggtaagttgc aggatttcat
    21961 gaaaattatc cgtgacgatc cggcagtgga taatgtcacc ggctttacag gcggttcgcg
    22021 agtgaacagc gggatgatgt ttatcaccct caagccacgc gacgaacgca gcgaaacggc
    22081 gcagcaaatt atcgaccgtc tgcgcgtaaa actggcgaaa gaaccggggg cgaatctgtt
    22141 cctgatggcg gtacaggata ttcgcgttgg tgggcgtcag tcgaacgcca gctaccagta
    22201 cacgttgtta tccgacgacc tggcggcact gcgagaatgg gagccgaaaa tccgcaaaaa
    22261 actggcgacg ttgccggaac tggcggacgt gaactccgat cagcaggata acggcgcgga
    22321 gatgaatctg gtttacgacc gcgacaccat ggcacggctg ggaatcgacg tacaagccgc
    22381 caacagtctg ttaaataacg ccttcggtca gcggcaaatc tcgaccattt accagccgat
    22441 gaaccagtat aaagtggtga tggaagtgga tccgcgctat acccaggaca tcagtgcgct
    22501 ggaaaaaatg ttcgttatca ataacgaagg caaagcgatc ccgctgtcgt atttcgctaa
    22561 atggcaaccg gcgaatgccc cactatcggt gaatcatcag ggattatcgg cggcctcgac
    22621 catttcgttt aacctgccga ccggaaaatc gctctcggac gccagtgcgg cgatcgatcg
    22681 cgcaatgacc cagcttggtg tgccttcgac ggtgcgcggc agttttgccg gcacggcgca
    22741 ggtgttccag gagacgatga actcgcaggt gatcctgatt atcgccgcca tcgccacggt
    22801 gtatatcgtg ctgggtatcc tttacgagag ttacgtacat ccgctgacga ttctctccac
    22861 cctgccctcg gcgggcgttg gagcgctgtt ggcgctggag ctgttcaatg ccccgttcag
    22921 cctaatcgcc ctgataggga tcatgctatt aatcggcatc gtgaagaaaa acgccattat
    22981 gatggtcgat tttgcgcttg aagcccaacg gcacggtaac ctgacgccgc aggaagctat
    23041 tttccaggcc tgtctgctgc gttttcgccc gattatgatg actaccctgg cggcgctgtt
    23101 tggtgcgctg ccgctggtat tgtcgggcgg cgacggctcg gagctgcggc aacccctggg
    23161 gatcaccatt gtcggcggac tggtaatgag ccagctcctt acgctgtata ccacgccggt
    23221 ggtgtatctc tttttcgacc gtctgcggct gcgtttttcg cgtaaaccta aacaaacggt
    23281 aaccgagtaa atgacaaatc ttcccgacag cacccgttgg caattgtgga ttgtggcttt
    23341 cggcttcttt atgcagtcgc tggacaccac catcgtaaac accgcccttc cctcaatggc
    23401 gcaaagcctc ggggaaagtc cgttgcatat gcacatggtc attgtctctt atgtgctgac
    23461 cgtggcggtg atgctgcccg ccagcggctg gctggcggac aaagtcggcg tgcgcaatat
    23521 tttctttacc gccatcgtgc tgtttactct cggttcactg ttttgcgcgc tttccggcac
    23581 gctgaacgaa ctgttgctgg cacgcgcgtt acagggcgtt ggcggcgcga tgatggtgcc
    23641 ggtcggcaga ttgacggtga tgaaaatcgt accgcgcgag caatatatgg cggcgatgac
    23701 ctttgtcacg ttacccggtc aggtcggtcc gctgctcggt ccggcgctcg gcggtctgct
    23761 ggtggagtac gcatcgtggc actgtatctt tttgatcaac attccggtgg ggattatcgg
    23821 tgcgatcgcc acattgctgt taatgccaaa ctacaccatg cagacgcggc gctttgatct
    23881 ctccggattt ttattgctgg cggttggcat ggcggtatta accctggcgc tggacggcag
    23941 taaaggtaca ggtttatcgc cgctgacgat tgcaggcctg gtcgcagttg gcgtggtggc
    24001 actggtgctt tatctgctgc acgccagaaa taacaaccgt gccctgttca gtctgaaact
    24061 gttccgtact cgtacctttt cgctgggcct ggcggggagc tttgccggac gtattggcag
    24121 tggcatgttg ccctttatga caccggtttt cctgcaaatt ggcctcggtt tctcgccgtt
    24181 tcatgccgga ctgatgatga tcccgatggt gcttggcagc atgggaatga agcgaattgt
    24241 ggtacaggtg gtgaatcgct ttggttatcg tcgggtactg gtagcgacca cgctgggtct
    24301 gtcgctggtc accctgttgt ttatgactac cgccctgctg ggctggtact acgttttgcc
    24361 gctcgtcctg tttttacaag ggatggtcaa ctcgacgcgt ttctcctcca tgaacaccct
    24421 gacgctgaaa gatctcccgg acaatctggc gagcagcggc aacagcctgc tgtcgatgat
    24481 tatgcaattg tcgatgagta tcggcgtcac tatcgccggg ctgttgctgg gactttttgg
    24541 ttcacagcat gtcagcgtcg acagcggcac cacacaaacc gtctttatgt acacctggct
    24601 tagcatggcg ttgatcatcg cccttccggc gttcatcttt gccagagtgc cgaacgatac
    24661 gcatcaaaat gtagctattt cgcggcgaaa aaggagcgcg caatgaagtt ctggcgaccc
    24721 ggtattaccg gcaaactgtt tctggcgatt ttcgccacct gcattgtctt gctgatcagt
    24781 atgcactggg cggtgcgtat cagttttgag cgtggcttta ttgattacat caagcatggt
    24841 aatgaacagc gattacaact gttaagtgat gcgcttggcg agcagtatgc gcagcatggc
    24901 aactggcgct tcctgcgcaa caatgatcgc tttgtctttc agatcctgcg ttcatttgaa
    24961 cacgataatt cggaagataa acccggcccg ggtatgccac cgcacggctg gcgtacccag
    25021 ttctgggtgg ttgatcaaaa caacaaagtg ctggttggtc cgcgagcgcc gattccacct
    25081 gacggtacac ggcgacccat tctggtcaac ggtgcggaag ttggcgcggt gatcgcctcc
    25141 cccgttgagc ggttaacgcg caatactgat atcaatttcg ataaacaaca gcggcaaacc
    25201 agctggttga ttgtcgccct ggcaacgtta ctcgcggcac ttgccacttt tctgctggcg
    25261 cgcggtttac tggcaccggt aaaacgactt gtcgatggca cgcacaaact ggcggcgggc
    25321 gatttcacta cccgcgtaac gcccaccagt gaagatgaac tgggcaaact ggcgcaagac
    25381 ttcaaccagc ttgccagcac actggagaaa aaccagcaaa tgcggcgcga ttttatggcc
    25441 gatatttctc acgaactgcg tacgccatta gcggtgctgc gcggtgaact ggaagccatt
    25501 caggatggcg tgcgtaaatt cacgccggag acggtggcgt ctttacaggc ggaggtcggt
    25561 acactgacca aactggttga cgatctccat cagttgtcga tgtctgatga aggcgctctc
    25621 gcctatcaaa aagcaccggt agatttgatc ccactgctgg aagtggcggg cggcgcattt
    25681 cgcgaacgat tcgccagtcg tggcctgaaa ctgcaatttt ccctgccaga cagtattacc
    25741 gtatttggcg atcgcgaccg tttaatacag ttattcaata acttactgga aaacagcctg
    25801 cgctacactg acagcggcgg cagcctgcaa atctctgccg ggcagcgcga caaaacggtg
    25861 cgcctgacct ttgccgacag tgcgccaggt gtcagtgacg atcagctaca aaaattgttt
    25921 gaacgttttt atcgcaccga aggttcccgc aaccgtgcca gcggcggttc cgggctgggg
    25981 ctggcgattt gcctgaacat tgttgaagca cataatggtc gcattattgc tgcccattcg
    26041 ccttttggcg gggtaagcat tacagtagag ttaccgctgg aacgggattt acagagagaa
    26101 gtatgaccga gttaccaatc gacgaaaaca caccgcgtat tttgatcgtg gaagatgaac
    26161 cgaagctggg gcagttgctc attgattatc tgcgtgctgc gagctatgcg ccgacgctta
    26221 tcagccacgg cgatcaggta ctgccgtatg tgcgccagac accaccggat ctgatcctgt
    26281 tagatctgat gctccctggc accgatggcc tgacgctgtg ccgggaaatt cgtcgttttt
    26341 ctgacattcc gatcgtgatg gtgacggcaa aaatcgaaga gatcgatcgc ctgctggggc
    26401 tggagattgg cgcagatgat tatatctgta agccgtacag cccacgggaa gtggtagcgc
    26461 gcgtcaaaac cattttgcgc cgttgcaaac cgcagcgcga gttgcagcaa caggatgctg
    26521 aaagcccgtt gattatcgac gaaggtcgtt ttcaggcttc atggcgcggt aaaatgcttg
    26581 acctgacgcc tgcggaattt cgtctgctga aaacgctctc tcacgaacca ggaaaagtgt
    26641 tctcccgcga gcaattgctc aatcatcttt atgacgacta ccgcgtagta accgaccgca
    26701 ccatcgacag ccacattaaa aacctgcgcc gcaagctgga atctctcgac gccgaacagt
    26761 catttatccg cgccgtttat ggcgtcggtt accgctggga agccgacgcc tgccgcatcg
    26821 tttagtttta gcgacattat tttgttagcc ggagatggcg ttcagaccag atccggcaac
    26881 attatcccac gcatggtcag caaactgaca ttatctctcc gccctggcag gcatcagaaa
    26941 atcgctcata ctttaatcgg taaacagcac ctttagatgc tgttttcgat acacaatttc
    27001 aatcaaggag tcattatggc tggttggttt gaactcagta agagcagtga taatcagttc
    27061 cggtttgtgc taaaagcggg caatggtgag actatcctca ccagcgagct ttatacctca
    27121 aaagcctctg cggaaaaggg catcgcgtcg gtgcgtagca acagcccgca agaagaacgc
    27181 tatgagaaaa aaacggcaag taacggcaaa ttctatttca atctgaaagc cgctaatcat
    27241 caaattatcg gctccagcca gatgtacgcc accgcgcaat ctcgtgaaac cggaattgcc
    27301 tccgttaaag ccaatggcac aagccagacg gtgaaagaca atacgtaatt acgatgccgg
    27361 gcgtgggttg ttggcgtccg gcgctttacc agatcccccc acagcgctac aatgcccgcc
    27421 cttaaagtgg gggcactccc ctaaccgctt catcaggtga agcggatctg acctgtcatc
    27481 agaacgagag aattatgttt aaaccggaac tcctttcccc ggcgggaacg ctgaaaaata
    27541 tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc gggccagccg cgttactccc
    27601 tgcgtgtgcg caacaacgaa ttcaaccacg aaaatcttca gctcggcatc aatgaagccc
    27661 acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac gccaagctga
    27721 aaacctttat ccgtgacctg aaaccggtgg tggaaatggg gccggatgcg ctgattatgt
    27781 ccgatccagg gctgattatg ctggtgcgtg agcacttccc tgaaatgccg atccacctct
    27841 cggtgcaggc taacgccgta aactgggcga cggtgaaatt ctggcagcaa atgggcctga
    27901 cccgcgtgat cctctctcgc gagctgtcgc tggaagagat tgaagagatc cgcaatcagg
    27961 tgccggatat ggagatcgag atcttcgttc acggcgcact gtgcatggcc tactccggtc
    28021 gctgcctgct ctctggctat atcaacaagc gcgatccgaa ccagggtacc tgcaccaacg
    28081 cctgccgctg ggagtacaac gtccaggaag ggaaagaaga tgacgttggc aacatcgtac
    28141 acaagtacga gccgattccg gtgcaaaatg ttgagccgac gctgggtatc ggcgcgccaa
    28201 ccgacaaagt gtttatgatc gaagaagccc agcgtccggg cgagtatatg accgcgtttg
    28261 aagatgagca cggcacttac atcatgaact cgaaagatct gcgcgccatc gcccatgtag
    28321 aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga aggccgtact aaatctttct
    28381 actattgcgc acgcaccgca caggtttacc gtaaagctat cgatgacgcc gctgcgggca
    28441 aaccgttcga taccagcctg ctggaaactc tggaaggtct ggcgcatcgt ggctataccg
    28501 aaggtttcct gcgtcgtcat actcacgacg attatcagaa ctacgaatac ggttattcag
    28561 tttctgaccg ccagcagttt gttggtgagt ttaccggtga gcgcaagggg gacctcgcgg
    28621 cggtagcggt gaaaaataaa ttctccgttg gcgacagcct tgagctgatg acgccgcaag
    28681 gcaacattaa ctttaccctt gagcacatgg aaaacgccaa aggtgaagcg atgccggtcg
    28741 caccaggcga tggttatact gtgtggctcc cggtcccgca ggatcttgag ctcaattacg
    28801 ctctgctgat gcgtaatttc tccggggaaa ccacgcgtaa cccacacggt aagtgattaa
    28861 tttcgattat ttttcccgga tggaaaattc ttagaaaccg atcacataca gctgcattta
    28921 ttaaggttat catccgtttc gctgaaaaac ataacccata aaatgctagc tgtaccagga
    28981 accacctcct tagcctgtgt aatctccctt acacgggctt atttttttat gcataagccc
    29041 tatctctggt aaccgtcttc cattgacca
//
LOCUS       DAZFZC010000064        28783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFZC010000064 DAZFZC010000000
VERSION     DAZFZC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.064"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(170..257)
                     /locus_tag="ACHKXP_003117"
     tRNA            complement(170..257)
                     /locus_tag="ACHKXP_003117"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Ser,seq:gga)
     gene            complement(510..728)
                     /gene="infA"
                     /locus_tag="ACHKXP_003118"
     CDS             complement(510..728)
                     /gene="infA"
                     /locus_tag="ACHKXP_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO7648071.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(1013..1717)
                     /gene="aat"
                     /locus_tag="ACHKXP_003119"
     CDS             complement(1013..1717)
                     /gene="aat"
                     /locus_tag="ACHKXP_003119"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO7648072.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEILRRDYLNYLNKMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1759..3480)
                     /gene="cydC"
                     /locus_tag="ACHKXP_003120"
     CDS             complement(1759..3480)
                     /gene="cydC"
                     /locus_tag="ACHKXP_003120"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO7648073.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     YRVSLTLRDVQFTYPEQSQQALKGISLQVNTGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3481..5247)
                     /gene="cydD"
                     /locus_tag="ACHKXP_003121"
     CDS             complement(3481..5247)
                     /gene="cydD"
                     /locus_tag="ACHKXP_003121"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO7648074.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLAEWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5370..6335)
                     /gene="trxB"
                     /locus_tag="ACHKXP_003122"
     CDS             complement(5370..6335)
                     /gene="trxB"
                     /locus_tag="ACHKXP_003122"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO7648075.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6880..7374
                     /gene="lrp"
                     /locus_tag="ACHKXP_003123"
     CDS             6880..7374
                     /gene="lrp"
                     /locus_tag="ACHKXP_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO7648076.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7509..11498
                     /gene="ftsK"
                     /locus_tag="ACHKXP_003124"
     CDS             7509..11498
                     /gene="ftsK"
                     /locus_tag="ACHKXP_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO7648077.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDVYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            11653..12264
                     /gene="lolA"
                     /locus_tag="ACHKXP_003125"
     CDS             11653..12264
                     /gene="lolA"
                     /locus_tag="ACHKXP_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO7648078.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12275..13618
                     /gene="rarA"
                     /locus_tag="ACHKXP_003126"
                     /gene_synonym="mgsA"
     CDS             12275..13618
                     /gene="rarA"
                     /locus_tag="ACHKXP_003126"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO7648079.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13709..15001
                     /gene="serS"
                     /locus_tag="ACHKXP_003127"
     CDS             13709..15001
                     /gene="serS"
                     /locus_tag="ACHKXP_003127"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO7648080.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15240..17684
                     /gene="dmsA"
                     /locus_tag="ACHKXP_003128"
     CDS             15240..17684
                     /gene="dmsA"
                     /locus_tag="ACHKXP_003128"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO7648081.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17695..18312
                     /gene="dmsB"
                     /locus_tag="ACHKXP_003129"
     CDS             17695..18312
                     /gene="dmsB"
                     /locus_tag="ACHKXP_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO7648082.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18314..19177
                     /gene="dmsC"
                     /locus_tag="ACHKXP_003130"
     CDS             18314..19177
                     /gene="dmsC"
                     /locus_tag="ACHKXP_003130"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO7648083.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGTLMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19212..19838)
                     /gene="ycaC"
                     /locus_tag="ACHKXP_003131"
     CDS             complement(19212..19838)
                     /gene="ycaC"
                     /locus_tag="ACHKXP_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO7648084.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRLSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20152..21300
                     /gene="ycaD"
                     /locus_tag="ACHKXP_003132"
     CDS             20152..21300
                     /gene="ycaD"
                     /locus_tag="ACHKXP_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648085.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21510..22940
                     /gene="ycaM"
                     /locus_tag="ACHKXP_003133"
     CDS             21510..22940
                     /gene="ycaM"
                     /locus_tag="ACHKXP_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7648086.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22941..23849)
                     /gene="ycaN"
                     /locus_tag="ACHKXP_003134"
     CDS             complement(22941..23849)
                     /gene="ycaN"
                     /locus_tag="ACHKXP_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7648087.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMSLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSCIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            23949..24539
                     /gene="ycaK"
                     /locus_tag="ACHKXP_003135"
     CDS             23949..24539
                     /gene="ycaK"
                     /locus_tag="ACHKXP_003135"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO7648088.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(24621..25361)
                     /gene="pflA"
                     /locus_tag="ACHKXP_003136"
     CDS             complement(24621..25361)
                     /gene="pflA"
                     /locus_tag="ACHKXP_003136"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO7648089.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(25553..27835)
                     /gene="pflB"
                     /locus_tag="ACHKXP_003137"
     CDS             complement(25553..27835)
                     /gene="pflB"
                     /locus_tag="ACHKXP_003137"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7648090.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(27890..28747)
                     /gene="focA"
                     /locus_tag="ACHKXP_003138"
     CDS             complement(27890..28747)
                     /gene="focA"
                     /locus_tag="ACHKXP_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO7648091.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         7103 a         7108 c         7529 g         7043 t
ORIGIN      
        1 agggggggat tgactcgctt cgctcgccct gcgggcagcc cactcactgc gttcgtggtc
       61 tgtccaactg gctgcgccag ttgtcgaacc ccggtcgggg cttctcaccc ccctggagtg
      121 cattatgcga aaaaaagctc gcactttcgt acgagctctt ctttaaatat ggcggtgagg
      181 gggggattcg aacccccgat acgttgccgt atacacactt tccaggcgtg ctccttcagc
      241 cactcggaca cctcaccaaa ttgttttgtc gcctgacctc atgggggcaa cggggcgcta
      301 ctatagggag ttggagtaaa acggtcaaga agaattttaa tgataattat tgtttgctca
      361 tactgtaaac aagttgtgca gtatatctac atcgagacaa gttacggact tatacttcca
      421 aagtacttca tacatatcac aaaataaaaa ggccggttaa accgaccttt tactcgttct
      481 ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac ggaagacaat gcggcctttg
      541 ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc ccgtcaggat gcggatgtag
      601 tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt gaccgttttc taactctacg
      661 cggaacatgg tattaggcaa cgtttcaaga acggtacctt gcatttcaat attgtcttct
      721 ttggccatct aatcctctgg ggtatcacta ccgtaatttg aaccggcaag ataatgccga
      781 agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa caaacaggtt cggcacatta
      841 ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg tataagggag cggtgagata
      901 aacgatgggc gttacctgac gcgaaaaatt ccttatcggc agcggggtaa tgagcgtaac
      961 caactctgcg accgcaatta taacactctg gggagaaatg tgccgaaaac attcattctt
     1021 gtggtgaaaa caagcatcgt ggtacccaga aattattcgg caatcgtccg aggcgcattt
     1081 tattgagata attaaggtaa tcccggcgga gaatttcgca ggcaccaagc gatgctgtgt
     1141 gatcgttaag gacctggcag tcgataagct taccgccatg accgataaat tcctcacaga
     1201 ataccagaag cgccgtttta gacgcatttt ccatccggct gaacatggac tcgccacaaa
     1261 atagcgttcc ctgggccacg ccgtacatac cgccgacaag ctcatcttca cgccagactt
     1321 caatggagtg ggcatggccg agttcgtgaa ggcgatggta ggcttcgacc acgccacgcg
     1381 tgatccaggt tccttcttcg cgatcgctgg cacagccttc aatgacctga ccaaaagcgt
     1441 aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg cttcatacta cggctgatat
     1501 gcagtgattc tggccatagc accgcgcgag gatcgggcga ccaccagagg atggggtcgc
     1561 ctggagaaaa ccacggaaaa ataccacgct ggtaagccat taacaggcgc gcagggctaa
     1621 gatcgccccc cagagccagc aggccgttag gctcacgtaa tgcgccttcc ggggaaggga
     1681 aggctattga atggcgagaa agctgaacca ggcgcatgac cgcaaaactc cacgcaagtc
     1741 ggatcgttca ataatagctt acaaaccctg cttaaactgg taataacgcc cctgtctggc
     1801 aagcagttct gcgtgagtac cttgctcaat aatttgcccg ttgtccatca ctattatttg
     1861 ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt aacaccgttt tctcatgcat
     1921 catttctgca agcaattcaa ggatctgact ttcggttgtg gcatctaagc cttctgtagg
     1981 ttcatccagc aacaccagtg gcgcatcatg taacagcgca cgggcgatag ccagacggcg
     2041 cagttcacca ccggagagct ggcgtccgcc ttcacctaac caactgttga gacctgcatc
     2101 ctcgagcagc ttctccagac caacgcgacg caagatctcc gccagagcct catcactact
     2161 gccaggcgag gcgagtaaca gattatcacg cagcgtggcg ctaaacagat gcactcgctg
     2221 aggaacaacg ctgatggtct gtcgtagagc cgcttcattc aggctggcta tggggctatc
     2281 gttaagcaaa atctcgccct gttgcgggtc ccatgcgcgg gtcagcagtt gtaacagtgt
     2341 tgatttgccg catccggttc gcccgagaat cgctatatgt tccccggtgt ttacctgaag
     2401 agaaatccct ttaagtgcct gttgagattg ctccggataa gtgaactgaa catcccgtaa
     2461 cgtcagcgaa acgcgatagg caacacgagt ttgggtatca gggaaggtga cttccggttt
     2521 ttgatccgtt aaatcagtga tccgaacggc agaggcaatg acttgcccca gatgctgaaa
     2581 tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac gcgcagaaga caaacagggc
     2641 aattaacgcg ccgggttgag cattgccgcc aacgccgcca gacgccatcc acagcatcag
     2701 gatcaccgct aacgcgccaa tgagcagcat tatcgcttgc gacaatgcgg tcagttcaga
     2761 ttgacggcgt tgcgcttcca gccattgaat ttctgtattc tctaattgcg tgcgataacg
     2821 atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc agccaggccg tcagttgttg
     2881 gcgatactgc ccgcgaagat gagtcagatt ttgcccggtg ctttttcccg cacgataaaa
     2941 cagcggtggc atcaggaaaa gcgtcagtaa cataatgccg cccagcgtaa aggcgagggt
     3001 gaaatcaagg aaacttaacc cgattgtcac caccataatc accacaaaag cgcccaccag
     3061 cggcgagata acgcgcaggt aaagatgatc gagcgtatca acatccgcca ccacgcggtt
     3121 gagcaattcg ccctgacgat agcgcgccag tccggcaggg gagaggggca acaatttgct
     3181 gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc gcgtcgtgac ttaccagacg
     3241 ttcaaaataa cgcccggcag tacgggtgat tgcggcacca cgcacgcccg cagcgggtag
     3301 catatagttg aagctgtaca gtccggcaac ccccgcaacc gctgaggccg agaggaacca
     3361 gccggaaagt gtcaacagac cgatactggc gagcagcgtc acaattgcca gcacaatacc
     3421 aagacttaac atccatttat gacgtttata cagtgccaga tagggtagca aagcgcgcat
     3481 ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat gggccaccag ccacacttaa
     3541 ttccgcgtaa cgtccttgct caataatccg gccatcctgc ataacccaaa tgacatccca
     3601 ctcagcaaga tcttctaact ggtgggtgac cattaacgtt gtctggcgca gagaggcggc
     3661 attcagcgcc tccattacgc gctgttcact gtgagcatca aggctggcag cgggttcatc
     3721 caacagtaat agcgaacagg gatttagtaa cgcacgggcc accgccacgc gctgcgcctg
     3781 ccccacggaa aggcgggcag cctggtcgcc aacaggcgta tcaacgcctt gtggcaggag
     3841 cggtagaaac tcgctgaccc aggcgttatc cagcgctgct tgtaattctt gttcgctggc
     3901 atcaggtcgc gccagtagta cgttatcccg taatgttgct gccggtaatt gtgggttttg
     3961 cccaacccag gagagatgtt tacgccatga ttctggtgat aaatcgcgta attctatccc
     4021 gttgattcgt aatgatccct gatatgagag aaaaccagaa agcgcgttca gcagtgagct
     4081 tttacctgaa ccgctgcgac caaccaacac tgcacgttgg cctgctggca aagtaaagtt
     4141 cagcggtccg gccagcgttt taccttccgg cgacgtgata aacagatcct cggcctcaat
     4201 ggtcagcgga tcggtcaatg ctaattccgc ctcaccgcgc tgcggatggg cgagcggggt
     4261 ttccataaac gttttcagac tgtcagctgc accaacagcc tgggctttag catgataaaa
     4321 cgtaccgaga tcgcgtaatg gctggaaaaa ctctggcgca aggatcaggg ccagaaaacc
     4381 cgcagccagc gtcacgccag tatcgtagtg accaaaatcc agctcgccga gataggaaaa
     4441 accaaagtag accgccacca gagcaattga cagcgaggta aaaaattcga gaatgccgga
     4501 ggataaaaac gccagtcgta gcacttccat tgtccgttgg cggaaatctt ccgaagcaga
     4561 acgaatactt tcaatttcag cttcaccacg accaaaaata cgcaatgttt ccatgccgcg
     4621 caggcgatcg aggaaatgcc cacttaagcg agcaagagcg agaaagttac gtcggttagc
     4681 atcggcagcc cccattccaa ccagcgccat aaacaacgga attagcggtg ctgtgcccag
     4741 cagaatgagc gccgcagccc agttagaggg gaagatcgcc accacaatca gcaacggcac
     4801 cgacactgcc agcgccattt gcggcagata gcgtgcatag taatcatgca tatcgtcaat
     4861 ttgctcgagt accagcgtcg cccagctccc cgcaggttta ccctgaatcc acgctggccc
     4921 tgcttgttgc agacggtcga gaacctgacg gcggatggca aagcggatat gctgcccggc
     4981 gtgataaccc acccgttcgc gtaaccagac cacccatgcg cgcagtacaa aggtcagaac
     5041 cagtaacgta aagggaagca gcagggcttc acggggaata ttctccataa tcatatgttg
     5101 cagaattcgc gccatgaacc aggcctgggc aatgatcaat atgccgctca caaagcccag
     5161 cagacgagaa atattcagcc aacgttggga gatgacgctt tgctgtttta accagcgggt
     5221 taactctttt tgacgagatt tattcattgc acgcttagca ggtgagttat cagaattatt
     5281 tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc catagtcgca tggtgtcgcc
     5341 ttctttactt ttgttactga tttgtaaaat tattttgcgt cagctaaacc atcgaggtag
     5401 cgttccgcat caagtgctgc catgcagcct gtaccggccg aagtaatggc ctggcgataa
     5461 atgtgatcca tcacgtcgcc tgcggcaaag acgccaggaa tgctggtctg ggtggcatta
     5521 ccatgaatac ccgactgtac tttgatgtag ccgttttcca gttccagctg cccttcgaaa
     5581 atcgcagtat tcgggctgtg accgatagca acaaacagac cggcaacgtc gagtgactcg
     5641 atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag tgacacccat ttgatcgccg
     5701 gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt tgccgttctc cactttatcc
     5761 atcaggcgct taatgaggat tttttccgcg cggaaaccgt cacggcggtg aatcagatgc
     5821 acttccgaag cgatgttaga cagatacagc gcctcttcaa ccgcggtatt gccgccgccg
     5881 atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc aggttgcaca agcagaaacc
     5941 ccacggcctt taaaggcttc ttcagagggc aggccgagat agcgtgcaga agctccggtg
     6001 gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc cattcagacg gaacggacgg
     6061 ttttgcagat ccaccttgtt gatatgatca aaaatgatct cagtttcaaa cttggtggca
     6121 tgttcgtgca tgcgctccat taataacgga ccggtcagat cgtttggatc gccaggccag
     6181 ttttccactt ccgtggtggt ggtcagttgg ccgccttttt ccatgccggt aatcagcaca
     6241 ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt atcccgccgg gcctgaaccc
     6301 aggataagca gtttactgtg tttggtcgtg cccatgagat ccccatagtt gttggcagac
     6361 aatgggcagg attgtaggga atttacagac gtaaaaaaag agtatgacga ttttgttaac
     6421 aatttgtgca atcggcagca tcgataagca ggtcaaattc tcccgtcatt atcacctctg
     6481 ctacttaaat ttcccgcttt ataagccgat taaatgatga ataaacgccc ctgttaatga
     6541 atatctggca tgttgtacta aaaatcgatg ttttgctttg acaatcccct ggtgttttgc
     6601 gaaaacattc gaggaagaaa aaaaacagta ttcttatatg cgcataacca tgcatgtaaa
     6661 taccatgttt accgtgctag tgaaatctac gtatggcgtg gacagacgcc attcgtgatg
     6721 tcgatagctg ccacaaggca acggtcttct caccgtagac ccaggcattg cgcgccgtga
     6781 atcttcatga tttcggtcta tcgtgacggg tagcgactct gaacagtgat gtttcagggt
     6841 cagacaggag tagggaagga atacagagag acaataataa tggtagatag caagaagcgc
     6901 cctggcaaag atctcgaccg tatcgatcgt aacattctta atgagttgca aaaggatggg
     6961 cgtatttcta acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag
     7021 cgtgtgcgtc ggctggaaag acaagggttt attcagggct atacggcgct gcttaacccc
     7081 cattatctgg atgcatcact tctggtattc gttgagatta ctctgaatcg tggcgcaccg
     7141 gatgtgtttg aacaattcaa taccgctgta caaaaacttg aagaaattca ggagtgtcat
     7201 ttagtatccg gtgatttcga ctacctgttg aaaacacgcg tgccggatat gtcagcctac
     7261 cgtaagttgc tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac acggacatac
     7321 gttgttatgg aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg ctaacacgga
     7381 acaggtgcaa aatcggcgta ttttgattac actcctgtta atccatacag caacagtact
     7441 ggggtaacct ggtactgttg tccgttttag catcgggcag gaaaagcctg taacctggag
     7501 agcctttctt gagccaggaa tacactgaag acaaagaagt cacattgaca aagttaagta
     7561 gcggccgccg ccttctggag gcgttgctga tccttattgt cctgtttgcc gtctggttga
     7621 tggctgcctt actaagcttt aacccttcgg accccagctg gtcgcaaacg gcctggcatg
     7681 aacctatcca taatttaggt gggatgcccg gtgcgtggtt ggcagatacg ctgttcttta
     7741 tttttggcgt gatggcttac accattcccg tcattattgt cggcggttgt tggtttgcct
     7801 ggcgtcatca gtccagcgac gaatacattg attattttgc cgtttcgcta cgcatcattg
     7861 gcgttttggc gctcatcctt acctcctgtg gtctggcggc aatcaacgct gacgatatct
     7921 ggtattttgc ctccggtggc gtcattggca gcttactaag cactacgcta caaccactgc
     7981 tacacagtag cgggggaact attgcgctgc tctgcgtttg ggcagcgggc ctgacgttgt
     8041 tcaccggttg gtcatgggtg accattgctg aaaaactcgg cggctggatt ttaaacattc
     8101 tcaccttcgc cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg
     8161 aagacgacga agagtatgaa gatgaaaatc acggcaaaca gcatgaatca cgccgtgccc
     8221 gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc ggaaaaattc attaatccga
     8281 tggggcggca aacagacgct gcgttgttct ccggtaagcg gatggatgat gacgaagaga
     8341 ttacctacac tgcacgcggt gtggctgccg acccggacga cgtcctattt tcgggcaatc
     8401 gtgcaacgca gccagaatat gacgtatacg atccattatt aaacggtgcg ccaattaccg
     8461 aacctgtcgc tgtagcagct gctgctacca cggcgacaca aagctgggct gcgccggttg
     8521 aacctgtgac tcagacgccg cctgttgcct ctgttgatgt tccacctgcg caacctacag
     8581 tagcctggca gcctgtaccg ggtccacaaa cgggagagcc ggttattgct cctgcaccgg
     8641 aaggttaccc acagcagtca caatatgcgc agcctgcagt gcaatataat gagccgctgc
     8701 aacaaccagt acagccgcag cagccgtatt atgcacctgc agctgaacaa cctgcgcaac
     8761 agccgtatta tgcccctgcg ccagaacaac cggtggcagg taacgcctgg caagccgaag
     8821 agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcagc
     8881 cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag cctgttgtgg
     8941 agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccgctttac tactttgaag
     9001 aagttgaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat caaccgattc
     9061 cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct tctgttgcag
     9121 cagtacctcc agtagaagcc gctgccgctg tttccccgct ggcatctggc gtgaaaaaag
     9181 cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata
     9241 gcggtggacc gcgtcctcag gtcaaagagg ggattggtcc gcagttgcca cgaccgaaac
     9301 gtatccgcgt gccaactcgt cgtgaactgg cgtcttacgg tattaagctg ccctcacagc
     9361 gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct ggcgatcagt
     9421 acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga
     9481 cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac gcagaagatg
     9541 cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa caacaacgtt
     9601 attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt gaattttcgc
     9661 caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca attgttgaac
     9721 ctgtacagca gccgcaacaa ccggttgcac cgcagcagca atatcagcag ccgcaacaac
     9781 cagttccgcc gcagccgcag tatcagcagc cacaacagcc ggttgcgccg cagccacaat
     9841 atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg caacaaccgg
     9901 ttgcgccgca gcagcagtat cagcagccac aacagccagt tgcgccacaa ccgcaggata
     9961 ccctgcttca tccgctgttg atgcgtaatg gcgacagccg tccgttgcat aaaccgacga
    10021 cgccgctgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata
    10081 cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg tctggctgat ttccgtatta
    10141 aagccgatgt cgtcaattac tctccggggc cggttatcac tcgctttgaa ttgaacctgg
    10201 caccgggcgt aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt
    10261 cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt
    10321 taccgaataa aaaacgacaa accgtttatc tgcgcgaagt tttggataac gccaaattcc
    10381 gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg
    10441 ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaaat
    10501 ctgtcggtgt gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc
    10561 gtttcatcat gatcgacccg aaaatgctgg agctttcggt ttatgaaggc attccgcatc
    10621 tgttaacgga agtcgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta
    10681 acgagatgga gcgtcggtat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt
    10741 ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga
    10801 agccgggtga cagtatggat gcccagcatc cggtgctgaa aaaagaacca tacattgtgg
    10861 tgttggttga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga
    10921 tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaactcagc
    10981 gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct
    11041 ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac
    11101 tgctgggtat gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc
    11161 atggtgcttt tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg
    11221 gtcgcccaca gtatgttgat ggcatcacct ccgacagcga aagcgaaggt ggtgcgggtg
    11281 gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg
    11341 aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg
    11401 cagcgcgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg
    11461 gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcattgc cggataaggc
    11521 gctgtagcgt cgcatccggc actctatcaa ctgaaaattc agtattttct tctttcctca
    11581 agctgattat tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt
    11641 tgaggaataa tgatgaaaaa aattgccatc acctgtgcat tactctcaag cttagtagca
    11701 agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc
    11761 ttccacgcca gcttcacaca aaaagtgact gacggtagcg gcgcggcggt gcaggaaggt
    11821 cagggcgatc tgtgggtgaa acgtccaaac ttattcaact ggcatatgac acaacctgat
    11881 gaaagcattc tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa
    11941 gctacggcaa cctggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc
    12001 aaccagtcca gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg
    12061 acgccgaaag ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc
    12121 acaatccatc agtttagcgc ggtggagcag gacgatcagc gcagcagtta tcaactgaaa
    12181 tcccagcaaa atggggctgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc
    12241 acggtagatg atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc
    12301 ggataatact tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat
    12361 cggccagcaa catttgctgg ctgcggggaa gccgttgccg cgcgctatcg aagccgggca
    12421 tttacattct atgatcctct gggggccgcc gggtaccggc aaaacaactc tcgctgaagt
    12481 gattgcccgc tatgcgaacg ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa
    12541 agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat
    12601 tctttttgtt gacgaagttc accgtttcaa caaaagccag caggatgcat ttctgccaca
    12661 tattgaagac ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgagct
    12721 taattcggca ctgctttccc gtgcccgtgt ctatctgttg aaatccctga gtacagagga
    12781 tattgagcaa gtactaactc aggcgatgga agacaaaacc cgtggctatg gtggtcagga
    12841 tattgttctg ccagatgaaa cacgacgcgc cattgctgaa ctggtgaatg gcgacgcgcg
    12901 ccgggcgtta aatacgctgg aaatgatggc ggatatggcc gaagtcgatg atagcggtaa
    12961 gcgggtcctg aagcctgaat tactgaccga aatcgccggt gaacgtagcg cccgctttga
    13021 taacaaaggc gatcgctttt acgatctgat ttccgcactg cataagtcgg tacgtggtag
    13081 cgcacccgat gcggcgctgt actggtatgc gcgaattatt accgctggtg gcgatccgtt
    13141 atatgtcgcg cgtcgctgtc tggcgattgc gtctgaagac gtcggtaatg ccgatccacg
    13201 ggcgatgcag gtggcaattg cggcctggga ttgctttact cgcgttggcc cggcggaagg
    13261 tgaacgcgcc attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaacgctgt
    13321 ctacactgcg tttaaagccg cgctggccga tgctcgcgaa cgcccggatt atgacgtgcc
    13381 ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa atgggctacg ggcaggaata
    13441 tcgttacgct catgatgaag caaacgctta tgctgccggt gaggtttact tcccgccgga
    13501 aatagcacaa acacgctatt atttcccgac aaacaggggc cttgaaggca agattggcga
    13561 aaagctcgcc tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg
    13621 ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc
    13681 catttaattc gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc
    13741 agacgcagtc gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg
    13801 cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg
    13861 taactcccga tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt
    13921 acgtctggaa gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc
    13981 tttacaggct gaaattcgcg atatcgcgct aaccatccct aacttgcctg cagatgaagt
    14041 gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc cgctggggta ccccgcgtga
    14101 gtttgacttt gaagttcgtg accacgtgac gctgggtgaa atgcactctg gcctcgactt
    14161 tgcagctgca gttaagctga ctggttcccg ctttgtggta atgaaagggc agattgctcg
    14221 catgcaccgc gcactgtcgc agtttatgct ggatctgcat accgaacagc atggctacag
    14281 tgagaactat gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcaactgcc
    14341 gaaatttgct ggcgatctgt tccatactcg tccgctggaa gaagaagcag acaccagtaa
    14401 ctatgcgctg atcccaacgg cagaagttcc gctgactaac ctggtgcgcg gtgaaatcat
    14461 cgatgaagat gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc
    14521 cggttcatat ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga
    14581 aatggtgcag atcgtgcgcc cagaagactc aatggcggcg ctggaagaga tgactggtca
    14641 tgcagaaaaa gtcctgcagt tgctgggcct gccgtaccgt aaaatcatcc tttgcactgg
    14701 cgacatgggc tttggcgctt gcaaaactta cgacctggaa gtatggatcc cggcacagaa
    14761 cacctaccgt gagatctctt cctgctccaa cgtttgggat ttccaggcac gtcgtatgca
    14821 ggcacgttgc cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc
    14881 tggtctggct gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg
    14941 tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta
    15001 atacccaatt tttctgaatc taaaaagcgc ctgcgggcgc tttttttgtc tccctttgat
    15061 accgaacaat aattactcct cacttacacg taatactact ttcgagtgaa aatctaccta
    15121 tctctttgat tttcaaatta ttcgatgtat acaagcctat atagcgaact gctatagaaa
    15181 taattacaca atacggtttg ttactggaat caatcgtgag caagcttgag tgagccatta
    15241 tgaaaacgaa aatccctgat gcggtattgg ctgctgaggt gagtcgccgt ggtttggtaa
    15301 aaacgacagc gatcggcggc ctggcaatgg ccagcagcgc attaacatta ccttttagtc
    15361 ggattgcgca cgctgtcgat agcgccattc caacaaaatc agacgaaaag gttatctgga
    15421 gcgcctgtac agttaactgt ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg
    15481 aaatcaaata tgtcgaaacg gacaataccg gcgatgacaa ttacgacggc ctgcaccagg
    15541 ttcgcgcctg cctgcgtggg cgttccatgc gtcgccgtgt ctacaatccg gaccgcctga
    15601 aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa attcgagcgc attagctggg
    15661 aagaagccta cgacatcatc gcgaccaata tgcagcgcct gatcaaagag tacggcaacg
    15721 agtctatcta tctgaactat ggcaccggta cgctgggcgg caccatgacc cgctcctggc
    15781 cgccgggaaa taccctggtc gcgcggctga tgaactgctg cggcggctat ctgaaccatt
    15841 acggcgacta ctcctccgcg caaattgcgg aaggtttgaa ctatacctac ggcggctggg
    15901 cagatggcaa cagcccgtcg gatatcgaaa acagtaagct ggtagtgctg tttggtaata
    15961 accctggcga aacgcgaatg agtggcggtg gggtgactta ctatcttgaa caggcacgcc
    16021 agaaatctaa tgcccgcatg atcatcatcg atccgcgcta taccgacacc ggtgccgggc
    16081 gcgaagatga gtggatccct attcgtccgg gaacagatgc cgcactggtt aacggtctgg
    16141 cgtacgtcat gatcactgaa aacctggtgg atcaggcatt cctcgataaa tattgcgttg
    16201 gctacgatga gaaaaccctg ccagccagtg cgccgaaaaa tggccactat aaagcttata
    16261 ttctgggtga agggccagat ggcgtggcta aaacgccgga atgggcctcg caaatcactg
    16321 gtgttccggc agacaaaatc atcaaattgg ctcgtgaaat cggtagtacc aaaccggcgt
    16381 ttatcagcca gggatggggc ccgcagcgtc acgctaacgg tgaaatcgca acccgtgcta
    16441 tctcgatgct ggcgattctg accggtaacg ttggtattaa tggagggaac agcggcgcgc
    16501 gtgaaggttc atacagctta ccgtttgtcc gtatgccgac cttggaaaac ccaatccaga
    16561 ccagcatttc tatgtttatg tggaccgatg ccattgaacg tggcccggaa atgacggcgc
    16621 tgcgtgatgg tgtacgcggg aaagataagc tggatgtgcc gatcaaaatg atctggaact
    16681 atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa ccgtacccat gaaatccttc
    16741 aggatgataa gaagtgcgag ctgattgtgg ttatcgactg ccacatgacc tcatcggcga
    16801 aatatgctga catcctgctg cctgactgca ccgcttccga acagatggac tttgcgctgg
    16861 atgcatcctg cgggaatatg tcttacgtga ttttcaacga tcaggtgatt aaaccgcgct
    16921 ttgaatgtaa gaccatctat gaaatgacca gcgaactggc aaaacgtctt ggcgttgagc
    16981 aacagtttac tgaaggccgt acccaggaag agtggatgcg gcatctgtat gcccagtcgc
    17041 gggaagcgat tcctgaactg ccaacgtttg aagagttccg caagcagggg atctttaaaa
    17101 agcgcgaccc acaagggcat cacgttgctt ataaagcctt ccgtgaagat ccgcaggcaa
    17161 acccactgac tacgccatcg ggcaaaattg agatttattc gcaggcgctg gctgacattg
    17221 ccgctacctg ggaattgcct gaaggcgatg tgatcgatcc actgccgatc tacacgccgg
    17281 gctttgaaag ttatcaggat ccgctgaaca aacagtatcc gctgcagctt acaggtttcc
    17341 actataaatc tcgcgttcac tcaacttacg gcaacgttga tgtgctgaaa gcggcttgcc
    17401 gtcaggaaat gtggatcaac ccgcttgatg cgcaaaaacg cggtatcaac aacggcgata
    17461 aagtcaggat ctttaacgat cgtggtgagg ttcatattga ggcgaaagtg acgccacgaa
    17521 tgatgccggg tgtggtcgca ctgggtgaag gtgcctggta tgacccggat acaaaacgtg
    17581 tcgataaggg cggttgtatc aacgtactga ccactcaacg tccgtctcct ctcgctaagg
    17641 ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt gtaaggagta accgatgaca
    17701 acccagtatg gattttttat tgattccagc cgttgcaccg gttgcaaaac ctgcgagctg
    17761 gcctgtaaag actacaaaga tttgacgcca gaagtcagct tccgccgcat ttatgaatat
    17821 gctggcggcg actggcagga agataacggt gtctggcacc agaacgtgtt tgcctactat
    17881 ctgtcgattt catgtaacca ctgcgaagat ccggcttgta ctaaagtctg cccgagcggt
    17941 gcgatgcata aacgtgaaga tggttttgtt gtggtcgatg aagatgtgtg cattggctgc
    18001 cgctactgcc atatggcttg cccgtatggc gcaccgcaat ataacgaaac gaaaggccat
    18061 atgaccaaat gcgatggttg ttatgaccgt gttgcggagg gtaaaaagcc gatctgtgtt
    18121 gaatcttgtc cgctgcgggc gctggatttc ggccctatcg acgagctgcg taaaaaacat
    18181 ggcgatctgg cggccgttgc gccgttgccg cgagctcact ttaccaaacc gaatattgtg
    18241 atcaaaccca atgccaatag ccgcccgacc ggggatacca ctggctatct ggcaaacccg
    18301 aaggaggtgt gagatgggaa gtggatggca tgaatggccg ctgatgatct tcacggtctt
    18361 cgggcaatgt gtagcaggtg gttttatcgt tctggctttg gcgctgctca aaggcgacct
    18421 gcgagcagaa gcccagcagc gtgttatcgc ctgcatgttt ggtttatggg tgctgatggg
    18481 cattggcttt atcgcctcta tgctccatct tggttcacca atgcgcgctt ttaactcgct
    18541 caaccgggta ggggcttcag cactcagtaa cgaaatcgcc agcggttcga tcttttttgc
    18601 cgtaggcggc atcggctggt tgctggcaat gctgaaaaag ctgtcaccgg cattgcgtac
    18661 gctgtggctg atagtgacga tggttcttgg cgtcatcttt gtctggatga tggtgcgtgt
    18721 gtataacagc attgataccg ttccgacctg gtacagcatc tggacgccga tgggcttctt
    18781 cctgacgatg tttatgggcg gcccgttact gggttacctg ttgttgagtc tggctggcgt
    18841 cgatggctgg gcgatgcgtc tgctgccagc aatttctgta ctggcactgg tagtgagtgg
    18901 cgtggtgtca gtgatgcagg gcgcagagct ggcgactatt catagttctg tgcagcaggc
    18961 cgcagcgctg gtgccggact atggtacgct gatgtcctgg cggatcgtgc ttttggccgt
    19021 tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact
    19081 ttctgtatca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg
    19141 tttgcatatg accgtgggga tggccgtcgc aagctaatca taacaaccgg ggtttcggcc
    19201 ccggttctct tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa
    19261 tcaggaatgt ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcgcga
    19321 tgcagctcgc acgccacgcc gaaccatgtc atcaattgcg caccagcttg cgacaagcga
    19381 tcccatgccg aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacgacaaag
    19441 acgtcaaacc cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc
    19501 acaccggcaa taattaactg ttttttacct gtcgctttga cagcttttac aaaatcttcg
    19561 ttatcccagg cgttaatatt tcccgggcga gcaatgtaag gtgtatcggg gaattgtgct
    19621 ttcagttctg gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt
    19681 ggtaagttga aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg
    19741 ggttcgatat cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacggca
    19801 gcatcatttt tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca
    19861 gtggtacgtc tgaaatcgat cagacgcgat gcattgctct gaaagcatag acgggaaata
    19921 tgagtttgct gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttagt
    19981 tcaataaaaa caatcagatg agactaatca tctcttcagc attagcgtta tgaatgagta
    20041 atataaatcg ataataccgc cgctgggata tcgcgtattt tcacccattg acaatgtttt
    20101 tggcggtggc atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg
    20161 tatacccggc ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgattgcg
    20221 gtgttaaata cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta
    20281 ggcgttgtca gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat
    20341 gtcattaagc gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct
    20401 ggctgtgccg gccttggcct gatgattgga ttctggagct ggttggcttg gcgttttgtc
    20461 gcgggcgtcg gctgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg
    20521 acgtcacgta accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg
    20581 tttttaggcc agttactggt cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg
    20641 gttacaggtt tgacgttggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag
    20701 caggctgaaa accatgattc gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg
    20761 cttggcgtga atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg
    20821 ccgctgtacc tcaatcacaa aggggtgagc aatgccagca ttggtttctg gatggcggta
    20881 ctggtcagtg cgggtatcct tggacaatgg ccgattggac gtctggcgga taagtttggt
    20941 cgactgctgg tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt
    21001 agccaggcgg cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat
    21061 ccggtggcga tggcatgggc ttgcgagaaa gttgaacatc atcaactggt ggcgatgaac
    21121 caggccttac tgttgagcta tactgtggga agtctgcttg gcccgtcatt taccgctatg
    21181 ctaatgcaga atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc
    21241 tatttgctga tgctgctgcg caacgccggt catacgccga aacccgttgc tcacgtgtaa
    21301 atgaattcaa gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc
    21361 tgacttgctt tattccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt
    21421 ttgcatttcg ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg
    21481 cccctttaat tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagttgc
    21541 gatggtacaa cattgcgctg atgtctttta tcactgtctg gggttttggc aacgttgtta
    21601 ataactatgc caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcactct
    21661 atttcacacc ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg
    21721 gcggggtcag tacctggatt aaacacacga tgggacccgg actggcttat ctcgccgcgt
    21781 ggacctactg ggtggtgcat attccctatc tggcacaaaa accccaggca attctgattg
    21841 cgctcggttg ggcgatgaaa ggcgacggtt cgctaatcaa agaatattca gtcgtagcgt
    21901 tacaggggtt aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga
    21961 aatcgctgaa aatcgtcggt tctgtggcag ggattgctat gtttgttatg tcgctcctgt
    22021 atgtggcgat ggcggtaacc gcgcctgcaa ttactgaagt gcatattgcg accacaaaca
    22081 ttacctggga aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc
    22141 tggttttcgc ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc
    22201 caggaaaaga atttccaaaa gggatgttat gcctggcggt gatggttgcg gtttgtgcca
    22261 ttctgggctc gctggcgatg gggatgatgt ttgattcgcg taatatcccg gatgacttaa
    22321 tgaccaacgg tcagtattac gcctttcaga agctgggcga gtattacaac atgggtaata
    22381 ctttaatggt gatttacgcc attgcgaata ccctgggaca agtagcggcg ctggtattct
    22441 cgattgatgc cccgcttaaa gtgctattag gtgatgctga cagcaaatat attccagcca
    22501 gtttatgtcg taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtac
    22561 tggtggcgat tctgattatg ctgccgactc tcggcattgg tgatatgaac aatctctata
    22621 aatggctgtt gaaccttaat tcggtagtga tgccgctgcg ttatttatgg gtatttgttg
    22681 catttattgc agtcgttcgc ttggcgcaga aatataaacc agaatatgtc tttattcgta
    22741 ataagcctct ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc tttgcctgtt
    22801 tgacggggat cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc
    22861 tgaatgttgc aacgccgttt gtgctggtag gattaggact gatattcccg ctgctggcgc
    22921 gtaaagcgaa tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc
    22981 gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttggataa
    23041 tagatctgga agcctggacg ctctgtgctc cagtcttcca gtacacattc taaacgacca
    23101 gtatcaaggt actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa
    23161 acggcctcca gttcagcatc aacatcatcc agaatgatat ttcctgcgac agcaatttcc
    23221 agctcctttg caaattgcca gtggaaaggt ttgccgctgg gataacgaaa aacaacacat
    23281 tgatgattaa gcagatcatg aggatggcgg ggtttgccat atcttgcaaa gtactctggc
    23341 gttgctgcta cacataattt cactggcggg ccgatcgcca cgctgatcat atctttttcg
    23401 acgatgcaac taagtcgaac gccagcgtca aagccctgtt ggacgatatc gacaagagag
    23461 tcgtcagttg tgagctcgac tttaatatca ggatattcac gtgtgaaacc aaccagtaaa
    23521 gacattaaaa atatacgtgc agctacccgg gcggcattga ttttgagcgt tcctgtcggt
    23581 gtcagacgaa aatcattcat ctcatctaac atgatctgaa tttcgtcgaa agctgggcgc
    23641 aatcgttcat agagattaga acccgcttcc gttaacgata cgcttcgggt tgtccgattg
    23701 aataagcgaa ttttaagacg ttgttccagt gttttaatac tatggctaat ggcggacgag
    23761 gataagccta actcatcgcc cgctgcacga aagctttgat tacgggccac ggcaaagaaa
    23821 gtggcaaagt cagacatatt catccgcatt agtgaaaatc cttcattaac tcatcctgaa
    23881 tcatcacgtt tacaggatta tgccgcctgc gtaaagtgcg ctccagaact taacgtggag
    23941 gtaaaattat gcagtctgaa cgtatttatt tggtatgggc ccatcctcgt catgattcat
    24001 tgaccgcaca tattgctgat gcgatccatc agcgggcaat ggagcggaaa atacaggtga
    24061 cggaactcga tttatatcgg cgtaatttca acccagtgat gacgccggaa gatgaaccag
    24121 actggaagaa tatggataaa cgttattctc cagaggttca tcagctttat tcagagctgc
    24181 ttgaacatga cacgttagtg gtggtttttc ctctctggtg gtacagcttc ccggcaatgc
    24241 taaaaggata tattgacaga gtatggaata atgggctggc ttatggagat gggcacaaat
    24301 taccattcaa taaagttcgt tgggtggcgc tggttggagg agacaaagaa tcatttgtcc
    24361 agatgggctg ggaaaaaaat ataagcgatt atttaaaaaa tatgtgcagt tatcttggta
    24421 ttgaagatgc cgatgtcact ttcttgtgta atacagtggt attcgatggg gaagaacttc
    24481 acgcgagcta ttatcagtcg ttattatctc aggtacggga tatggtagat gcactataag
    24541 atgtgttaaa aacgctgtag cagaatgaag cgcggaataa aaaagcggca actcaataaa
    24601 gttgccgctt tacggggaaa ttagaacatt accttatgac cgtactgctc aagaatgcct
    24661 ttcacgcgtt ccatggtctc tttcttcggt ggtttaacac cgtcgagttt gtactcttca
    24721 cccattgcca cccatttgtg tttgcccagc tcgtggtagg ggagaagctc gattttctca
    24781 acgttgccca tatcacgggt aaattcaccg aggcgatgcg ctgaatcgtc atcgtcagac
    24841 cagcctggga caacaacgta gcggatccac accttcacat ttttgttcgc cagatattta
    24901 gcgaactcca gcgtgcggtg gttggaaact ccaaccagat tttggtggat ctcgtcgttc
    24961 atctgtttga gatcgagcat taccaggtcg gttacttcca gcagttcatc aatcaccgga
    25021 tcgtaacgac gaacaaaacc gttggtgtcc agacaggtat gaatgccttc ttttttgcag
    25081 gcgcggaacc agtcacgaac aaactcagct tgcaggattg cttcaccgcc ggatgcggta
    25141 acgccgccgc cggaagcgtt cataaagtgg cgataggtca ccacttcctt catcaaatct
    25201 tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt cgcggttatg acaatacagg
    25261 cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa tacccgggcc gtctacggtt
    25321 ccacaggatt caaaggagtg aatgcgacca ataactgaca ttgcggtgtt tctccagatg
    25381 tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc tgttttggca
    25441 gtcaccttaa agtatagata gctgacaaaa aaggctctcg cgctaaaaaa ggccccactt
    25501 tcgtggagcc tttattgtac gctttttact gtacgatttc agtcaaatct aattacatag
    25561 attgagtgaa ggtacgagta ataacgtcct gctgctgttc tttagtcagc gagttgaaac
    25621 gtactgcgta gccagataca cggatggtca gctgcggata tttttccggg ttttccatcg
    25681 cgtcgagcag catttcacgg ttcatcacgt taacgttcag gtgctgacca ccttcgatgg
    25741 atgcttcgtg gtggaagtaa ccatccatca gaccagccag gttggtctta cgaacttcgt
    25801 cgtctttacc cagtgcgttc ggaacgatag agaaggtgta ggagatacca tctttagcgt
    25861 aagcaaacgg cagtttagca acggaagtca gagaggctac tgcacctttc tggtcacgac
    25921 cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg tctggggtgt
    25981 tacccgtttt cttaccatac acaacgttag aagtgatggt cagaacagac tgagtcggga
    26041 tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct accaggtcaa
    26101 cagccaggtc atctacacgc ggatcattgt taccaaactg cgggtattcg ccttcgattt
    26161 cgaagtcgat agccagaccg tcttcgtcac gaatcggttt aactttcgca tatttgattg
    26221 cagacaggga gtcagcagca acggacagac cagcgatacc acacgccatg gtgcggataa
    26281 cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg tgcatgtagt
    26341 ggatgatgtt cagtgcagtg atgtactgtt tagccagcca gtccatgaag tgatccatgc
    26401 gctccatcac ttcatcatag ttcaggacat cgcctttgat cggttcagac ttcggaccaa
    26461 cctgcatttt cagtttttcg tcaacgccgc cgttgattgc gtacagcatg gttttcgcca
    26521 ggtttgcacg cgcaccgaag aactgcattt gtttaccaac gatcatcggg cttacgcagc
    26581 aagcaatagc gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg ttctcatact
    26641 gcagagaaga ggtgtcgatg gacactttag cggcgaattt cttgaagttc agcggcagtt
    26701 tttcagacca cagaatggtc atgttcggtt ccggagacgg acccatggtg tacagggtgt
    26761 tcaggaaacg gaagctgttt ttggtaacca gggtacgacc gtcgaggccc ataccaccga
    26821 tagattcggt tgcccagatc gggtcgccag agaacagttc atcgtattcc ggagtacgca
    26881 ggaagcgaac catacgcagt ttcatgacca ggtggtcaac catttcctgc gcttcttgtt
    26941 cggtgatctt gccagctttc aggtcacgtt cgatgtacac atccaagaag gtggaggtac
    27001 gaccgaagga cattgcagca ccgttctgag acttaacagc agccaggtag ccgaagtaag
    27061 tccactggat agcttcctga gcgttggtag ccggaccaga gatgtcgtag ccgtatttcg
    27121 cagccatttc tttcatctga cccagagcgc ggtgctgttc agcgatttct tcgcgcagac
    27181 ggatagtctg ttccaggttt acgccgtttt ccagatcagc ctgcagagaa gtgaactgtg
    27241 ccagtttgtc tttcatcagg tagtcgatac cgtacagcgc aacgcgacgg tagtcaccga
    27301 tgatacggcc acggccatat gcatctggca gaccggtcag aacaccagat ttacggcaac
    27361 gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta cggtattcag
    27421 tgaagatttt tttgatcatc ggatccagtt cgcggttgta cgctttgcag gaaccttcga
    27481 tcattttgat accaccgaac gggataagag cacgtttcag cggagcttca gtctgcagac
    27541 caacgatttt ctcaagctgc ttgttgatgt agccagcgtc gtgagaggtg atggtggaag
    27601 caacagcggt gtcaaagtca actggcgcgt gagtgcggtt ttccagttta acgccttcca
    27661 ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc agccaggaag gactcgtcac
    27721 cctcgtacgg agtgtagttt ttctgaatga agtcacggac gtttacttca ttctgccagt
    27781 cacctttggt aaaaccttcc caggctgtgg ctaacttttc attaagctcg gacatgtaac
    27841 acctaccttc ttaagtggat tttttattta ctgcgtactt cgacaaccat taatggtggt
    27901 cgttttcacg caggtaaatg acccagtatg tcaacccaac caacaaacca ccaccgataa
    27961 tgttgccgat cgtaaccgga atcaggttat cagtgatgaa attcatcacg gtcaggtgag
    28021 aaaaattttc cggtgcagaa ccgactgcgg tccaaaattc cggggatgcg aagtcgcgga
    28081 ttacaatacc catcgggatc ataaacatgt ttgcgatact gtgctcaaaa ccgctggcaa
    28141 caaacatcgc gaccggcagc accataatga acgctttgtc catcaggctg cggccagaat
    28201 aactcatcca tactgccaga cataccatca ggtttgccag gataccaaga cagacggcct
    28261 caataaaagt atggtgcact ttgtggtcgg cggtttgtag gacgtttagt ccccattgac
    28321 catttgcggt catatactcg ccggaaagcc acattaaaag tacaaacagc agtgcgccga
    28381 ccaggttgcc aaaatagaca tttagccagt ttttcgccaa ctgaccccag gtgatgcgcc
    28441 cactcgcctt agcaacaaca atcaacacgg tggaagtaaa gagatcggct ccgcagacaa
    28501 cacaaagaat cagccccaga gagaagcaaa tgccgccaac cagttttgcc atgccgaagg
    28561 gcattgtgcc tgtgccagtg gttgctgtga tatagaagac gaatgcgatt gagatgaaaa
    28621 caccggcggt aatcgccaga tagaaagtct taagcggatg tttcgttgct ttatagacac
    28681 ccgcctcttc ggccactttg gccattgcag caggaagtaa aagatcaaaa gggttgtcag
    28741 ctttcacact aactctctct ttattaagtc ggcgacgaga tac
//
LOCUS       DAZFZC010000065        28594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFZC010000065 DAZFZC010000000
VERSION     DAZFZC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.065"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..344
                     /locus_tag="ACHKXP_003139"
     CDS             <1..344
                     /locus_tag="ACHKXP_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO7648092.1"
                     /translation="FEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPFMP
                     FWQVTVLTGGLWGISWGCAMWFIYWGPSGMVVGEAIIISITGGFLFGLCMASFHWWRR
                     KVNRLPPWDDV"
     gene            complement(364..954)
                     /locus_tag="ACHKXP_003140"
     CDS             complement(364..954)
                     /locus_tag="ACHKXP_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648093.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQKRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(954..1211)
                     /locus_tag="ACHKXP_003141"
     CDS             complement(954..1211)
                     /locus_tag="ACHKXP_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648094.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            1485..1976
                     /locus_tag="ACHKXP_003142"
     CDS             1485..1976
                     /locus_tag="ACHKXP_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN family nuclease"
                     /protein_id="HFO7648095.1"
                     /translation="MGNYSDFETDFIERTLALIDQYNNMIEGKPFPEQYNYTLTLNCL
                     LGLIVMPRERAVSYLPSDRLTPELKAEIGLNESQLPGEEMNLRELIHKMRNSVAHFCV
                     QVESISDARLVDQIIFKETHGAGRAYAIFSAPELLPFLKYYAALLIANMRRHRGVPTT
                     DVV"
     gene            1988..2746
                     /locus_tag="ACHKXP_003143"
     CDS             1988..2746
                     /locus_tag="ACHKXP_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648096.1"
                     /translation="MFTLDCSTRSQALLSLSSGFGCSVMELKKVLLSLDLEQIYETDH
                     SIMIDSRQYLREYVCRELGIPGEFTTAYWFHGTRTSADNTFENGLLALNQTESLVMDM
                     LVNLAPDAEVKEKLQAWNFHAGVPDHLFRTRTRDKMHWGPYGHLVREVHLHARKLWQH
                     DYVRLPELVEDVCNAYKKKYGQDLTGHYLEVLKPCIVCFRADIDYEKGALEAALSYAY
                     TSVRELPPDSGAVFGIDRHGKSVSVDEIVNVEFI"
     gene            2968..4560
                     /locus_tag="ACHKXP_003144"
     CDS             2968..4560
                     /locus_tag="ACHKXP_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648097.1"
                     /translation="MTSIIVTSLLKTGPCLSSVLVEEMLKTSGVNRDTARKQISRAAS
                     AGQIHCVDKLFPKRERFIYLKQEYGTGRFWSSLNAALLDTGSAYGLALSCLRARGGIL
                     PVRNFSAACGSPVAMKNRLSWKSVLDGLLQYKMVRVVTLPGLGECVALTEKNDNGYLR
                     ALHPLKARLLTESVLMKSLSQWVRNNGIISYDTLRTREELNSDQTPCVANFDFDVTAA
                     SYLNPLLQFSRSGEIRPGFFVCDMLLGCKLSLVHLQPFITKCRSINSLRNSPRCLFMF
                     IADEYSEEAFLEMKRSGIIPATPENLFGKDFADALFQLRDLVGSITLSLKDNIAAIDD
                     IMSKLANIAGATNQLQGDLFEYIVAETVRIDSKDVEVGKICKSLKGETAECDVLSLNG
                     HAKITFIECKGYKPYSTVRHEDVKKWIGKQVPVFFSYAKREYPNAEINVQLWTTGKLC
                     DDSRESLRKFQENNLTNQRYNITVMEPHEVCARIKATRNDALIRVFDKHFLSYPEKIV
                     RRKHVPDPVRLAGHDEAIEFDF"
     gene            4595..5719
                     /locus_tag="ACHKXP_003145"
     CDS             4595..5719
                     /locus_tag="ACHKXP_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HFO7648098.1"
                     /translation="MDYTKNQHVLSQWVLRNFRSDDTALYAKEKQRVWCHTVYMTPEK
                     ENVLHTQPLPISSVAVSKNCFRLIDAETGQPFDIEDELGVYERLLAGIVNDIIHEHNF
                     SRLRHTHPDDFPVEKLTSFAVMQLMLNLYNPQNKNPYKQEMLEQFHADIQDHLSEYIH
                     SINQLPHSNPELMDDHFYRKILRVANSASSDENKCRALFVLFGLLSTLNKPTLYDKIN
                     KLRNNIFTGIHTIEVIHTGHDFDSTEPRPAFAIAPNVFCIYKDENRIYLPLSHNITLC
                     FITGTALHFRPRIDVFSPRPERLKCCHDRSLNFYNVSFDYIDNVMTTIDMYNVGTSST
                     FYSAYELSKLNEYLDKQQQDHDYYYTPENPVKWQPAQTVT"
     gene            5719..6009
                     /locus_tag="ACHKXP_003146"
     CDS             5719..6009
                     /locus_tag="ACHKXP_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="korC"
                     /protein_id="HFO7648099.1"
                     /translation="MKAVNENDFRELIEAKQFNGYTLVSGEDWQIPTAHEILLVRGLI
                     PLTDIQLANRLDVDERTIRKWKTGQTRMVFTTWCCLCWLAGLGMPLDNHLSG"
     gene            6582..7583
                     /locus_tag="ACHKXP_003147"
     CDS             6582..7583
                     /locus_tag="ACHKXP_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HFO7648100.1"
                     /translation="MADSVNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLENHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQALETLGLSSGGDTKD
                     IFLRQLVQQMSHIDHLLKKDWYLLETRPESPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMIRQKQHLHNLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLFMPLTPDHVTHYNALQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
     gene            complement(7757..8101)
                     /locus_tag="ACHKXP_003148"
     CDS             complement(7757..8101)
                     /locus_tag="ACHKXP_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HFO7648101.1"
                     /translation="MKNLFDTEGTVLFPFQTEIHFIWTIYTTAKTLYVSACGHIGQKQ
                     YWSACLCILLLMAYVGLCAAVVWAVVPWLKAFIVSKFAVSALWVTPVKSLLLRGIGKM
                     VGAICRKFTLLK"
     gene            complement(8599..8853)
                     /locus_tag="ACHKXP_003149"
     CDS             complement(8599..8853)
                     /locus_tag="ACHKXP_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648102.1"
                     /translation="MKGRQSRYVTGGESFAEIARRPAGTVVILSLNTDLEDALREVSK
                     SLKSAFCRCGRKCQLSAGTSEGPFSGRRQGVATHLFVSVL"
     gene            complement(8903..9829)
                     /locus_tag="ACHKXP_003150"
     CDS             complement(8903..9829)
                     /locus_tag="ACHKXP_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648103.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADIDRVITDLATHARKPKAT
                     FLREFLEDSFRDVIDGFALKNPLIASLDDELASYLGAEVMEKRYQSHYITRWNQEYQK
                     LLGISTEEELRRVVLTNTPFLHARADQVLKGWKKIPRGISLTFSLFAEIAGRDRETID
                     SAWNRIFYSQLREKKHRFYRDIDVIRALKKQHAVCGYSWTRDDITVRIYRPDDYGYGA
                     WRVLLVLDESVITQTWNIPFPELDGRRFTTDPGYDALISTAPDSWDKAFRFVDGICEL
                     HLYSNGVEEDQNPTPLPAVAEALIEAVVHDLL"
     gene            10361..10774
                     /locus_tag="ACHKXP_003151"
     CDS             10361..10774
                     /locus_tag="ACHKXP_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648104.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            10776..11561
                     /locus_tag="ACHKXP_003152"
     CDS             10776..11561
                     /locus_tag="ACHKXP_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7648105.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFAMPEADAVALIKQLERR"
     gene            12163..12795
                     /locus_tag="ACHKXP_003153"
     CDS             12163..12795
                     /locus_tag="ACHKXP_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7648106.1"
                     /translation="MAWIVGFISQKGGVGKSTKARALAREASACGIKTKLADLDLEQA
                     TSAEWHRRRLSAGLSPVASVEVFSTAKQAIESAGDFDLLILDGPARASKGTSEIAKVA
                     DLIVQPTGASLDDLVPAIKVFNALVKEGIPRSKLVFALSRVGTEAEELDARAYISEAG
                     YETLSGCLFEKPAYRKAMNSGLAVTETRYKGLNERADELIQALIDKIGEE"
     gene            12795..13169
                     /locus_tag="ACHKXP_003154"
     CDS             12795..13169
                     /locus_tag="ACHKXP_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648107.1"
                     /translation="MADASKLRTKRTRSGLGVPPGPDEVATSLNAPEIAPAVAATDVQ
                     RQQADSSPVHHEPVVTQTVTEVYTRRDGRSARKTNRTLAFATRVTPAFDQEIRDIAER
                     EGLKLVEVLEKAVEAYKEKQGY"
     gene            13408..14382
                     /gene="parM"
                     /locus_tag="ACHKXP_003155"
     CDS             13408..14382
                     /gene="parM"
                     /locus_tag="ACHKXP_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO7648108.1"
                     /translation="MRIFCDDGSTNVKLAWFEGKTLKSAVSVNSFRHNWKVEGLGSSR
                     TYNYLLDGRKYTYDPVSEDAISTTHIEYQYSDTNVLAVHHALLNSGIEPQEIDLTVTL
                     PISEFYTADCQKNTLNIERKIRNLMREVTLNKGGTFTIKSVEVMPESLPAVFTRLVAD
                     NVGQYEKSLVIDLGGTTLDVGVIVGQFEDVSAVHGNPDIGVSMVTKATLTALKMASSD
                     TSPMIADELIKNRKNLDFVGQVVNEVSKLNLVLDTIDTAINKLGELVVDDLLQYRNVN
                     RVYIVGGGAALIADAVRKAWNHLGEKVVLMDEPQTALVQAIARFKAEE"
     gene            14386..14778
                     /locus_tag="ACHKXP_003156"
     CDS             14386..14778
                     /locus_tag="ACHKXP_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7648109.1"
                     /translation="MYDGEERKKLSLYLHPEDSADCLALAEIETVPRKKRGELYRQAL
                     ITGLIMHQLDERIPAVLTALFTRELNADEVISQIARITGWKPSSGDLKEVLKALGGLQ
                     STVSPEHSQDDGEQARLKAARVKMQNLI"
     gene            complement(14862..15041)
                     /locus_tag="ACHKXP_003157"
     CDS             complement(14862..15041)
                     /locus_tag="ACHKXP_003157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648110.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLHMW
                     ESPSFRAGSCQPALH"
     gene            16064..17788
                     /gene="ltrA"
                     /locus_tag="ACHKXP_003158"
     CDS             16064..17788
                     /gene="ltrA"
                     /locus_tag="ACHKXP_003158"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO7648111.1"
                     /translation="MTEQATTCKGASLLNGDSWHSINWRQCYREVRRLQARIVKATRE
                     GKHGKVKSLQWILTHSFSGRAVAVRRVTDNSGKRTPGVDGQTWSSPEVKFLAINLLKR
                     RGYKPQPLKRVYIPKSNGKSRPLGIPTMKDRAMQALYLLALEPVAEVTADQRSFGFRT
                     GRSTADAIAQCFCVLAQKTSAEWVLEGDIRGCFDNISHQWLIDNTSTDRQILTKWLKA
                     GYREKGQLFPVNSGTPQGGIISPVLANIALDGLEALLASEFKKRTVKGRLVNPKVNYV
                     RYADDFIITGESKELLESQVLPVVRRFMAERGLMLSPEKTKITHIEEGFDFLGQNIRK
                     YGGKMLIKPSKANVSSFLKKIRAVIKGNKAMDQLTLIRMLNPMIKGWAAYHQHIVAKV
                     AFNKVDNEIWLALWRWAVRRHPNKGKKWIRKRYFHQQGARNWSFSTATGELLANGKPQ
                     YANLRKAIDTPINRFKPIKIAANPFDPQWEMYFEERCADKMRHKLQGRKKLIQIWFEQ
                     RGRCPICDERITSDSQWQVHHIIRRVDGGSNCLSNLIMLHPMCHTLVHAKGIHVVKPA
                     HESGLRKA"
     gene            17865..18767
                     /locus_tag="ACHKXP_003159"
     CDS             17865..18767
                     /locus_tag="ACHKXP_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7648112.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            18764..19075
                     /locus_tag="ACHKXP_003160"
     CDS             18764..19075
                     /locus_tag="ACHKXP_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648113.1"
                     /translation="MKYDGNGRETARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLPSERRRLRYSTVGLTRYRT
                     R"
     gene            19151..19834
                     /locus_tag="ACHKXP_003161"
     CDS             19151..19834
                     /locus_tag="ACHKXP_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO7648114.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECGYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            19835..20056
                     /locus_tag="ACHKXP_003162"
     CDS             19835..20056
                     /locus_tag="ACHKXP_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648115.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            20037..20504
                     /locus_tag="ACHKXP_003163"
     CDS             20037..20504
                     /locus_tag="ACHKXP_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7648116.1"
                     /translation="MRALKIEKETRMYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQ
                     ALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            20549..21319
                     /locus_tag="ACHKXP_003164"
     CDS             20549..21319
                     /locus_tag="ACHKXP_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648117.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEIYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRDVLREVVREARAEIRSMADALAKIRG"
     gene            21733..22158
                     /locus_tag="ACHKXP_003165"
     CDS             21733..22158
                     /locus_tag="ACHKXP_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7648118.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDVGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            22205..22627
                     /locus_tag="ACHKXP_003166"
     CDS             22205..22627
                     /locus_tag="ACHKXP_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7648119.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            22624..22815
                     /locus_tag="ACHKXP_003167"
     CDS             22624..22815
                     /locus_tag="ACHKXP_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648120.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            22886..23200
                     /locus_tag="ACHKXP_003168"
     CDS             22886..23200
                     /locus_tag="ACHKXP_003168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648121.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDTVTVSGCLLLRP
                     LRLPAAWRHPVVRPDSDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPV
                     AP"
     gene            23585..24112
                     /locus_tag="ACHKXP_003169"
     CDS             23585..24112
                     /locus_tag="ACHKXP_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7648122.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            24170..24395
                     /locus_tag="ACHKXP_003170"
                     /pseudo
     CDS             24170..24395
                     /locus_tag="ACHKXP_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            24454..26412
                     /locus_tag="ACHKXP_003171"
     CDS             24454..26412
                     /locus_tag="ACHKXP_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7648123.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTAEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMVMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQMDATEHTTNLADAA"
     gene            26467..26901
                     /gene="psiB"
                     /locus_tag="ACHKXP_003172"
     CDS             26467..26901
                     /gene="psiB"
                     /locus_tag="ACHKXP_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7648124.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPV
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            26898..27617
                     /locus_tag="ACHKXP_003173"
     CDS             26898..27617
                     /locus_tag="ACHKXP_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7648125.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            27614..28210
                     /locus_tag="ACHKXP_003174"
     CDS             27614..28210
                     /locus_tag="ACHKXP_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648126.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
BASE COUNT         6723 a         7174 c         7902 g         6795 t
ORIGIN      
        1 cctttgagca gaaaaaagcc cgggccattg ccctgatgga cagcaaaaag atgtggcgga
       61 gcaattatgc ccctcctctt ctgcgtattc tatggcggct gggtatccgg cttccgccat
      121 taccctttat gccgttctgg caggtcacgg tgctgacggg gggcttatgg ggcatttcct
      181 ggggatgtgc aatgtggttt atctactggg ggccgtcagg aatggtggta ggtgaggcca
      241 ttattataag tatcacgggt ggttttttgt tcggactgtg catggcctct ttccactggt
      301 ggcgcaggaa agttaaccgg ctgccaccat gggatgatgt gtaaaccgtg cacaggcgtg
      361 ttcttacgga gcggcacttt catttatctg tacgcgaact tctattcctg cgtcatgaca
      421 ggcctgcagc cactgcgcca cttccagcgg atcgccctcc cggcgtacca ccctgccttc
      481 tttattccat aactgcagac aggtgctgcc gtcgagacgc accacaaaat ccccacggca
      541 ggcctgatac gtcatgccgt tcagctccac cgccagatca gtggcagaag tgccgtctgc
      601 gggcagacct gctgccggga ccagcggcag tacgacgact tctgccgcac gctgttctgc
      661 ccggaggcgc ttctgttctg ccagcagcag aggctgtgcc acggcccggc cggcgtccgt
      721 cagctccact gccagctgca gattcggggc tcgcagggtg cgcagccagc cggcatcctc
      781 caggcgacgg caggaggcgc gcaggtttgg cccgtagact ggggcttccc cgcttcgctc
      841 cagcacccgt tctatgtccc gcgtcagcac ggggcctggt cgtttttcat caagggcagc
      901 cagcacaatc agcactctcc gctgcagtgg cgacggtcgg cggctctggg tcattacctg
      961 tcatcctccg gccagatgag gtttctgagg cgctgaaagt cggcgtcaat gagctcctgt
     1021 ctctttatgg cattcattga ggcatagcca tttatggcca gttcatgcca gaagatcagc
     1081 acggctccgg ctttggctga ctcgcagaga taaatcgcgc cctggctgtc cagattgcat
     1141 tcccggatgc gtttttccgc aaaccgggtc agttgttcat agctgagtga aaacgtcatt
     1201 tcctgatcca tcctgcacgc tccatctgat aatgttctga caggtaaaaa tcatatcctg
     1261 catgatttgt gtgctcctga tttctgataa tatcagatat tgtttaattt ttaatgatga
     1321 aaatcatatc tgatatgatt ttatgtgaac tgcttaatta tcatatcgtt tgcccgtttc
     1381 ccgccgtgca gatccggttt ccggcaaaat cggaaagtaa ggatatgaac cgtagggata
     1441 tctgaatggt atagttagat tttctgcttc cgaaggagga tttagtggga aactattcag
     1501 actttgagac tgattttatc gaaagaacac tggccctaat cgatcaatat aacaatatga
     1561 ttgaggggaa gcccttccct gagcagtaca actatacgct tactctgaac tgtcttttgg
     1621 gcctaatcgt gatgccgaga gagcgcgctg tttcttacct cccgtcggat cgtctaacgc
     1681 cagagcttaa agctgaaatt gggttaaacg agtcgcagtt accgggtgag gaaatgaact
     1741 taagagaact gattcataaa atgcgaaatt ctgtggcgca tttctgtgtt caggttgaat
     1801 ctatcagcga tgcacgccta gttgatcaaa tcatcttcaa agagacgcat ggtgctggcc
     1861 gggcttatgc aattttcagt gccccggagc ttctcccatt tctaaaatat tacgcggctt
     1921 tgttgatcgc caatatgaga cgccatcggg gtgtccccac gactgacgtt gtgtaaggaa
     1981 tgcaaccatg tttactctcg attgttcaac acgatctcag gcgctgcttt cactttcatc
     2041 cggttttggt tgtagtgtaa tggagctaaa aaaggttttg ttatctctcg atcttgagca
     2101 gatttacgag acggatcatt ccattatgat cgattcacga cagtatctga gagagtacgt
     2161 atgtagggaa ctgggtattc cgggtgagtt caccaccgca tactggtttc atggtacccg
     2221 cacttctgct gataatactt ttgaaaatgg tttacttgca ctgaaccaaa ctgagtcact
     2281 ggtgatggac atgctggtaa atctggctcc agacgctgag gtcaaagaga aattacaggc
     2341 ctggaatttt catgcaggcg tacctgatca cctgtttcgg acccgaacca gggataaaat
     2401 gcactgggga ccatatggtc accttgtgcg ggaggtccat cttcatgcac gtaaactctg
     2461 gcagcacgac tatgtacggc tgccggagct ggtagaggat gtctgcaatg cttacaaaaa
     2521 aaaatatggg caggatctta caggacatta tcttgaggta ctgaaacctt gcatagtctg
     2581 tttcagggcg gatattgatt atgaaaaggg tgcgcttgaa gctgcgctta gttacgcata
     2641 cacgtctgtt agagaattgc caccggatag tggcgctgtg ttcggtattg atcgacatgg
     2701 taaatccgtt tctgtcgatg aaattgtgaa tgttgaattc atatgagctt gatgactgaa
     2761 gcccgtaggg ttatagatgt tatcgatact tcttttttta gtggcaaaat tttctacagg
     2821 aaaatgaagt ttgtgacaga taatctaaag ggcctcattt atgtcattta acctattgta
     2881 tttacagctc taaactgcag gctttgtcgt ttcgatgctt gagtgtattg ttattattaa
     2941 atacgcagtg tgacaagtga gcaaaagatg acatcgatta tagttacttc gttactcaaa
     3001 accggtcctt gccttagctc tgtacttgta gaggaaatgc tgaaaacctc gggcgttaac
     3061 cgtgataccg cgcgcaaaca gatttcccgg gctgcttctg cagggcaaat ccactgtgtt
     3121 gacaagcttt tccccaagcg cgaacgattt atttatctga aacaagagta tggtaccggg
     3181 cgcttctggt ccagtctcaa tgcggccctt cttgataccg ggtcggccta cggtctcgcc
     3241 ctgagttgcc tccgggcacg cggggggatc ctgccggtca gaaatttttc tgcagcctgc
     3301 ggttcgccgg tagcaatgaa aaacagatta tcctggaagt cagttctgga cggtctgctt
     3361 cagtacaaaa tggtcagggt cgttacgtta ccgggtctgg gggaatgtgt tgcgctgaca
     3421 gaaaaaaatg ataacggtta tctgcgcgcg ctccatcccc tgaaagccag actactcacc
     3481 gagtcagttc tgatgaaatc cctgagtcag tgggtccgta acaatggcat catcagttac
     3541 gacacgctgc gtacgcgaga ggaactcaat tcggatcaga ctccctgcgt ggcaaatttt
     3601 gattttgacg tcactgctgc atcctatctt aatcctcttc tgcagttctc ccggtcaggt
     3661 gaaatccggc ctggtttctt tgtctgcgac atgctcttgg gctgtaagct cagtctggtg
     3721 catctccagc cttttattac gaaatgtcgc tcgataaaca gcctccggaa cagccccaga
     3781 tgcctgttta tgtttatcgc tgatgaatac agtgaagagg catttctgga aatgaagcgt
     3841 tccgggataa tacccgccac gccggaaaat ctcttcggga aagactttgc tgatgcactc
     3901 tttcagctac gggatcttgt cggctccata acgctcagct taaaagataa tatagccgca
     3961 atagatgaca tcatgtccaa gctggcgaat atagccggcg cgactaatca gcttcagggc
     4021 gatttatttg aatatattgt cgctgagacc gtgcgtatag acagtaagga cgttgaggtt
     4081 ggaaaaatat gtaagtcgct taagggggag acggcggaat gtgatgtact cagtttgaat
     4141 gggcatgcga aaattacctt cattgagtgc aagggttata aaccctattc gacggtgaga
     4201 catgaagatg taaaaaaatg gattggtaag caggttccgg tattcttcag ctatgccaaa
     4261 agggaatacc ccaacgctga aattaatgtt cagttatgga ctacaggaaa gctctgcgat
     4321 gattccagag agtcgcttcg taaattccaa gaaaataact taactaatca gcgttataat
     4381 ataaccgtca tggagccgca tgaagtttgt gccagaataa aggcgaccag gaatgacgcg
     4441 cttatccggg tttttgataa gcattttctc agttatccag aaaaaatcgt tcgccgtaaa
     4501 catgtgcccg acccggtcag gcttgcaggt catgatgaag caatagaatt tgatttctga
     4561 accggtcggc tcgtttaatc agagagaaaa cgttatggat tacacaaaaa atcagcatgt
     4621 gctttcacag tgggtgttac gcaactttcg cagcgatgac acagccctgt atgcaaagga
     4681 aaagcagcgg gtatggtgtc ataccgtgta catgacgccc gaaaaagaaa atgttctcca
     4741 cacgcaacct ctgcctatat ccagtgtggc agtatcgaaa aactgctttc gtcttattga
     4801 tgctgagaca ggacaacctt tcgatattga agacgaactg ggtgtctacg agcgcctgct
     4861 cgctgggatc gtgaacgata tcattcatga gcacaatttc tcacgccttc gtcacacaca
     4921 tcccgatgat ttcccggtag agaagctcac cagcttcgcg gtgatgcagt taatgctaaa
     4981 tctgtacaat ccgcaaaata aaaatccgta taagcaagaa atgcttgaac agttccatgc
     5041 cgatatacag gaccatttat ccgaatacat ccattctata aatcagttgc cccacagtaa
     5101 cccagagtta atggatgatc atttctatcg taaaatactg cgtgttgcaa actcagcctc
     5161 ttccgatgag aataaatgca gagcactttt tgtgttattt ggccttttat ccaccctgaa
     5221 taaaccgacc ctttacgata aaatcaacaa attaaggaat aatattttta ccggtatcca
     5281 taccattgag gtcattcata caggtcatga tttcgacagc actgaaccga gacctgcatt
     5341 cgcaatagca cctaatgttt tttgcattta taaagatgaa aacagaattt atcttccgct
     5401 atctcataac ataaccctgt gcttcattac tggcactgca ttacatttca gaccccgcat
     5461 agatgtcttc tctcctcgtc ccgagcggct gaaatgttgc cacgaccgga gtcttaattt
     5521 ttataatgtt tcgtttgatt acattgacaa tgtgatgaca actattgata tgtataatgt
     5581 gggtacatcc agcacatttt attcagccta tgagttgagc aaacttaatg aatatctgga
     5641 taagcagcag caggatcatg actattatta tacgccggaa aatcctgtta aatggcagcc
     5701 tgcgcagacg gtcacctaat gaaagccgtt aacgaaaatg atttccgcga gctgattgag
     5761 gctaaacaat ttaatggcta tacgctggtc agcggagaag actggcagat acccacggcc
     5821 cacgaaatcc tgctggtcag gggccttatc cccctgactg acatccagct ggccaaccgc
     5881 ctggatgtgg acgaacgcac tatccgcaaa tggaaaaccg gacagacccg gatggtgttc
     5941 accacctggt gctgtctgtg ctggctggcc ggactgggga tgccgctgga taaccatctc
     6001 agcggttaaa gagacgcttc cagaggcgtt taagaatgaa tcgtctctgg tatcgccagc
     6061 gtgagcggta gatatgggga aggtcgtcat agttcttgaa gaaccaggtc caacctatat
     6121 aggcatttct cactaagaga atgagagatc ccgctgtcaa aatgccataa cccatggcga
     6181 ggataaatac agttatacaa tgttccttat gtaaaactgt atttaggaaa tgcaatgcgg
     6241 cctttaaggc cggttgacaa aaaaacctga aaaagttttc ctttcctatt tgttgcaatt
     6301 taagtctcag tcagtcctga attttgcatt ttttgcaaca atccccacgt agcaacaatt
     6361 cttaactttt tgtgattgtc aggcaataat ccctctcttc tgcctttgag atccgttttc
     6421 tgctataacc ggagatttaa atttcatcag tactgacagg gtgttaaaca caggtattat
     6481 cgaacggttc tgttttctaa tgttatgccg caaaccagct gcccatgccg tcaatacaga
     6541 tgcaccggtg cgcctgcgtg gcttttcagg gagataaagt tatggccgat tcagtcaatc
     6601 tgcagaccga tgtggtcaga cagcatacgg ttccccgttt tttactgaag catttcagta
     6661 cgccgggaaa gggtaagcgt cagcgtttat acgcctttga caaggctgca gggcgcgcct
     6721 atgccaccac gccggatgat gcaaccgtcc gcaacacgtt ctacaacctc gaaaatcacc
     6781 cggaccgtct gagtcttgaa ccactgctgg ggatttacga gcaccacgcg gcaccggtaa
     6841 ttgcagccct gctggcgcac agggatattc gcaggctgac agatgacgaa cgctacaggc
     6901 tggccgtgtt tgttgccgtg cagcgcgcac gaaccttcgg cgaactggag cgtatctcag
     6961 gcatgatttc ggtgctcacc gataagatgg aggcaatcgg ctcgactaaa gagcaggccc
     7021 tggaaaccct gggcctgtcg tccgggggcg ataccaagga tatttttctg cggcagctcg
     7081 tccagcagat gtcacatatt gatcacctgc tgaaaaagga ctggtacctt ctggaaacca
     7141 ggccagaaag tccgttttac gtatcagaca atcccgtcgt gcttaagaac agcaatgact
     7201 tcggtccgta cggtaatctt ggtctggccg ttcgcgggat acagatttac ctccccctgt
     7261 catcgacgct gatgctggcc atgtattgcc cctccatccg tgaacagatg attcgtcaaa
     7321 agcagcatct tcacaatctg cttgccagag cgccgcatct tatcccccgt cacattcggc
     7381 cttttgaaag actggaacat atccggaggt atacggacta tcttttcatg ccgcttaccc
     7441 cggatcatgt cacgcattac aacgccctgc aggtggagtt cgcggagcag tacgtgttct
     7501 gcggtgaaaa ggatttctct ctggttgaga gaatgctggc cgatagtgaa cgctaccgga
     7561 caggcccgcg gtttacgttt tagttccaat tccggcctca cctgtgccgg aaaatgacgg
     7621 cgggagcccc tgttgtacag aggagttttg ccgtgttaca cccacgcgaa agcggcagtg
     7681 gccacaggga ccaccgccgt aaggagatgg tgaatgcctg ttgacattca ccatccgttg
     7741 gtgtagcttg ctgctcttac tttaatagag taaactttcg gcagatggcc cccaccatct
     7801 taccgatacc cctgagcaac aaactcttta ctggcgtgac ccagagtgct gaaaccgcaa
     7861 atttcgacac aataaaggcc ttaagccagg gcactacagc ccagaccacc gcagcacaga
     7921 gacccacgta ggccataaga agcaaaatac agagacaggc gctccagtat tgcttctgtc
     7981 cgatgtgccc acaggcactg acgtaaaggg ttttagctgt ggtgtaaatc gtccagataa
     8041 aatggatttc agtctggaat gggaatagaa ccgttccctc ggtgtcgaac aaatttttca
     8101 taaagccctc ctgggcaggt tgttgttaac actgatgtgt taatcttgat catcttcttg
     8161 acctcttttt tcagataaaa ggcacccggc gcctcacaca cgtgaacgct gttgataccg
     8221 tacagaatgt tttctctttc aattctgcta tgatgtgttc attcgcatcc tcctgtctgt
     8281 cataccgatt ttaagctcgt cccgggtgct cccaggaaaa aaatccaaaa aaatccgggc
     8341 cggaatggtg ataaccggtc tggcgggaaa taaacggatt tgttggagtg gagcgggacg
     8401 gaacaatact atgaaagtaa tgttaacaga aaaccacgcc atcgtcaagg cgcagctacc
     8461 cattaaagta actatagcat gctattgttg atattccttt agcaaaaagt gctatccatc
     8521 ccgtcgctgt accttcattt tcgcccggag cggggcagcc aggcgaatac ctttttcagg
     8581 tttttgcccc atgccccctc agagtaccga cacaaacaga tgggtggcca cgccctgccg
     8641 gcgcccgctg aacggccctt cagatgttcc ggctgacagc tggcattttc gtccgcaacg
     8701 gcagaaggcc gacttgaggg atttggacac ctcccgcagc gcgtcttcca gatcggtgtt
     8761 caggctgagt atcaccactg tcccggccgg gcgacgggcg atctccgcga aactctcccc
     8821 accggtaaca tagcggctct gtcgaccttt cattatgcct cctgatgatg cagttgtggt
     8881 tgtgcccgta cacccgggtc cgttacagta gatcgtgcac caccgcttca atcagcgcct
     8941 cagcgacggc cggcagcggg gtcggattct gatcttcttc cacgccattg ctataaagat
     9001 gaagctcaca aatcccgtcc acaaaacgga aggccttatc ccagctgtcg ggagcggtgc
     9061 tgatcagggc gtcatagccc gggtcagtgg taaagcgacg tccgtcaagt tccgggaacg
     9121 ggatgttcca cgtctgggta atgacacttt catccagtac cagcagcacc cgccaggcac
     9181 cgtagccgta gtcatccggg cggtaaatac gcacggtgat gtcatccctg gtccagctgt
     9241 agccgcagac ggcatgctgt ttcttcaggg cacgaataac atcaatatcc cggtaaaagc
     9301 ggtgcttttt ctccctcagc tgtgagtaga aaatcctgtt ccaggcactg tcaatggttt
     9361 ccctgtcccg gccggcgatc tcggcaaaca gggaaaaggt cagggagatc ccgcgcggga
     9421 tctttttcca gcccttaagc acctggtcgg cacgtgcgtg caggaagggc gtgttggtca
     9481 gcaccacccg gcgaagctcc tcttccgtcg agatcccgag cagcttctgg tattcctggt
     9541 tccagcgggt gatgtaatgg ctctggtagc gtttctccat gacctcggcg cccagataac
     9601 tggccagctc gtcatcgaga gaggcgatga gcgggttttt cagggcgaaa ccgtcgatga
     9661 cgtcccggaa gctgtcttcc aggaactcac gcagaaacgt cgcctttggc ttacgggcgt
     9721 gagttgccag gtcggtgatc acccggtcga tgtcggcggg aatgttccgc agcgtgtacg
     9781 ttacgctctc tccaccgtcg tttgtcatgt catgttcttt ttcactcatt ccgtgtacct
     9841 tttcatgtgt ggatgcatta ctgcaatact catacactat cctgggttta agactattta
     9901 aatactgcga agcatatgtt tttataattc gtatgctttg atgtttattt tctgttcacc
     9961 ttcttttctg agatccgaaa acttccggtt caggatcttc ctctcccccc acataacgcc
    10021 acctcctgtc agcacaacat gtagtgctga attcagctgc taatgacact atatgttgtg
    10081 tcatctccct gacctgtgat gcgccgcgca ggggaggaaa acagcgatat gattatttcc
    10141 tcagcgtggt aaacttccgg aaagttgtga tattccggaa agtcggatct gacggaaacg
    10201 gctctccggt aatttaacgg cgtggttata tggatgcttg ttatcatggt aatgatgata
    10261 acggcatgat gttatcagac ggcgtgacgg taagggcagt gatgatggat gacgttatcg
    10321 catgaacgtc cctgcccgga aaagaaaaaa ggagtcaccc atgtttttta ttgagaatga
    10381 aggtcaggct gtcgccagaa cggattactg gcagtctgta caggcgcagg ccggatatgt
    10441 ctacctcagc tggaatgccg gcgcagccag gctgcttgtc ccggatgcgg caaaacattt
    10501 actcagggag atgcgggggg ctgagtacgt catcatcagt aagggaacgc tgcatggccg
    10561 cgatgcgctg gaactggtat ttgaagacgg cagcgatgcg ccgtttgtga tccacatgct
    10621 gagtgagcag tgcgatcgcc tgctccccga aaacaaccag ggagggggtt ttgttgtcac
    10681 cgtctggacg cgtggcggta accagctccg ttatccggga aagtaccgtg ttgtggaaaa
    10741 cctgcccgac gtttccccgt ggagtgaaca ctgatatgca gcacctgccg gcaccgatcc
    10801 accatgcccg ggatgctgct cagcttcctg ttgccatcga ttatccggca gcgctggcac
    10861 tccgccagat gtcgatggtt catgatgaac tgccaaaata cctgctggcc cctgaagtga
    10921 gcgccctgct ccattacgtc ccggatctgc gccgcaagat gctgctggcc acactgtgga
    10981 acaccggtgc gcgcattaat gaagcactgg cgctgacgcg gggggatttt tcgcttgcgc
    11041 ctccgtatcc gtttgtgcag ctggccactc tgaagcagcg gacagaaaaa gccgccagga
    11101 cggcaggaag aatgcccgcc ggtcagcaga ctcaccggct ggttccgctt tccgacacct
    11161 ggtacgtcag ccagctgcag acgatggtgg ccacactgaa aatccccatg gaacggcgta
    11221 ataaacgaac aggcaggaca gagaaagcgc ggatctggga agtgacggac agaacggtca
    11281 ggacctggat tggggaggcg gttgccgccg ctgcagctga tggtgtgacg ttctctgtcc
    11341 cggtcacgcc acatacgttc cgccattcct atgcgatgca catgctgtat gccggtatac
    11401 cgctgaaggt cctgcagagc ctgatggggc ataagtccat cagctcaacg gaggtctaca
    11461 cgaaggtgtt tgcactggat gtcgcggcgc gccatcgtgt gcagttcgcc atgccggaag
    11521 ccgatgcggt agcgctgata aaacaacttg agaggcgtta aaaagccgta ttcgcggttc
    11581 agtcaagggg gagctggtgc atccatctgt ggaagataga gcaccaaact catagaggcc
    11641 acgttctcag gccagaacag tcttatatct ctggcgctga caacccatag tacttgcctg
    11701 gatgggaaac tcttcttgtg ctgagcgtaa gagccatctg ccggaacgct tcctcgccag
    11761 ttcgctcgct acgcacagtt acatggctgc ggcgagggct gcctgattct aataatgata
    11821 tttcagcatg atagcagcaa taaaatagat tgcagctatc atgctgtaca catacggcct
    11881 agcgctctgc ttatgttatg ctatctgcaa tcttgcggca atcttgcttc atctgtcgat
    11941 gcgattctga atgaggtttc ttttattgtt aacgtgacac aagtattcgg ctgtcaattc
    12001 cagcactctg ctaagtagca agatttaatc ttgctactag attttttatt gttgcaatct
    12061 tgctgcaagc tgttatcttg ttctggaaga gttactctac ctaacctcaa tctggtttat
    12121 atttatgttt gctgcaagat agcagcaagt aaagggggct aaatggcctg gatagttggt
    12181 tttatttctc agaaaggtgg tgtgggcaaa agcactaaag cgagagcgtt agcgcgtgaa
    12241 gcatccgcct gtggtatcaa aacaaaatta gctgatctcg acttggaaca ggcaacgagt
    12301 gctgagtggc atcgtagacg tctgtcagct ggtttgtcac cggttgcttc agttgaagtg
    12361 ttttcaacag caaagcaggc gatagaatct gccggcgatt ttgaccttct gattcttgat
    12421 ggccctgctc gtgcaagtaa aggtacttca gaaattgcta aggtcgccga ccttatcgtc
    12481 cagccaacgg gggcctccct tgacgatctt gtcccggcaa ttaaagtttt caacgcgttg
    12541 gtaaaagaag gtataccacg ttcaaaactg gtcttcgctt taagccgggt aggtaccgaa
    12601 gcggaagagc tggatgctcg tgcatatatc agtgaagcag gttatgaaac tttatctgga
    12661 tgcctttttg agaagccagc atacagaaaa gcgatgaatt caggactcgc tgtgacagaa
    12721 acacgttata aaggtttaaa cgagagagct gatgaactta tccaggcact catcgataag
    12781 attggagaag agtaatggct gatgcgtcaa aacttagaac taaaagaacc cgtagtgggt
    12841 tgggggtacc cccgggcccc gacgaagtgg ctaccagctt gaatgcgcct gagatagctc
    12901 cggctgtcgc tgccactgat gttcagagac aacaggcaga ctcatcgcca gtgcaccatg
    12961 agcctgtggt tacgcaaact gtgaccgagg tatatacccg ccgcgatggc cgttctgcca
    13021 gaaaaacaaa caggacgctg gcatttgcaa cacgtgtcac acctgctttc gaccaagaga
    13081 tccgggatat cgcagagcgt gaggggttga aactggtgga agttctggaa aaagcagttg
    13141 aggcatataa ggaaaaacag ggctattaat ccgggatgca gcaatcttgc tgcattcttt
    13201 ttttgtgtgc aatcttgcag caagatttat gctagataaa tgctagattg ctgcaagata
    13261 aatgatataa tgctcaaatt gagtagttaa ctgccccagt gaagtacgca gcttgagtac
    13321 ttacctgtgg tttatgaacg tatttactta atgtgagtag ttattgggtg aagattaata
    13381 ctcaaattga gtataaatgg agatggtatg agaatttttt gcgatgatgg ttctacaaat
    13441 gtgaaacttg cctggtttga aggcaagacg ctgaaaagtg cggtatcagt gaactctttt
    13501 cgccataact ggaaagtcga gggcttaggc agcagtcgta cctataacta tttgcttgat
    13561 ggccgtaaat atacctacga cccggtcagc gaagatgcga tcagcacgac tcatattgag
    13621 tatcagtact ctgatacaaa tgtgttggca gttcaccacg cattactgaa cagtggaatt
    13681 gaaccccagg agatcgatct caccgttacg ttacctatca gcgagtttta cacggccgac
    13741 tgccagaaaa atacgcttaa cattgagaga aaaattcgca acctgatgcg ggaagtcacc
    13801 ctgaacaaag ggggaacttt taccattaaa agcgttgaag tgatgcctga atcactcccg
    13861 gctgtattta ctcgcctagt tgctgacaat gttggtcagt acgaaaaatc attggttatc
    13921 gatctcggtg gtacaactct ggatgtcggg gttattgttg gccagtttga ggatgtttcg
    13981 gctgtacatg gtaatcctga tattggtgta tcaatggtca cgaaagccac gctcactgca
    14041 ctgaaaatgg ccagcagtga taccagtcca atgattgctg acgagttgat caaaaacagg
    14101 aagaacctgg attttgtggg gcaggttgta aatgaggttt ctaaactaaa tctggtgctg
    14161 gataccattg atacagcgat aaataaactg ggcgagctag ttgttgatga tttgctgcag
    14221 tatcgaaacg taaaccgcgt atatattgtc ggtggcgggg ctgcactcat tgctgatgct
    14281 gttcgtaagg catggaacca ccttggcgaa aaggtggttc ttatggatga acctcagaca
    14341 gcgctggtcc aggctattgc acgcttcaaa gcggaggagt aatcgatgta cgacggcgaa
    14401 gaaaggaaaa aactttcact ctaccttcac ccggaggact ctgccgattg tcttgcacta
    14461 gctgaaatcg aaactgttcc ccggaaaaaa cggggggagt tatatcggca ggctttgata
    14521 accggcctga taatgcatca gcttgatgag cgaattcccg ccgtgttaac cgcgctattc
    14581 accagagaac tgaatgcgga tgaggtcatt agtcagattg ccaggataac aggctggaaa
    14641 ccctcctccg gtgatttaaa agaggtactg aaggcgttgg gagggcttca gagtacagtt
    14701 tcaccagaac actctcagga tgacggtgaa caggcccgtc tgaaagcggc tagggtgaag
    14761 atgcaaaacc taatctaaaa aagtctcgct ccgtgaaacc tgcaaaattt ctcggggtgt
    14821 gcggcttaat ttttagattt ccgcgtttcc cgtcatttga gtcaatggag tgctggttga
    14881 cagctccctg ctctgaagga cggggattcc cacatatgca gccagatttc cgggtagtgt
    14941 ggtttcagac gttgttcgat ttcattctgt agcgcctgaa gtgtgcctga tttgaggttt
    15001 tttgtgctct ggcggtcgaa aagaatttca atgcggatca tgttctcatc tcccttttat
    15061 tttgctgtat gcatatacag tattataata actgaatcca cacacagtca aatgtcatta
    15121 ttgtgactaa aaaacgctca attctctgag agtgtggctg tatgcctgct gtggacggct
    15181 gtgtccagct tatccacagc attttgcgca cggttctgtg aataaaatac ctggttaccc
    15241 aggccgtgcc ggcacgttaa ccgggctgca tccgatgcaa gtgtgtcgct gtcgccggcc
    15301 tcctcacccg gtcacgtttc gtcgttcctc ctccacgcgc tgcggcttcg gggccgcacc
    15361 tgcattcgta tgcggtcgcc cggttacagg tgcggcacgg cctgatggag gccgcatgtg
    15421 agaggagaat tcccatgcca aactggtgct caaatgtgcg acatgaagtc gcctgaataa
    15481 ttgttccagc ggagttcgat tccgtcaggg aacctgatgt tccgtcatca gtagcctact
    15541 gacacatgcg tcactggtaa cggtggggtg tgaagctgtc aggacaatga aaccggatct
    15601 tcggatcgca tgaaaccgtg aggttacatg taatctgcca gcatcaggcg gaggaggtct
    15661 aggctcggta gcatgactaa catatgtgaa ctgctgaagc gtcgttaacc agaacaagcc
    15721 aaagatgctg acaggcttga accaaaaggt atgtggttgg ttactcctct gtttgctagg
    15781 ggtacacgga caataaacca ccggtgttgt gagcagagtc taacctactg ttgttattca
    15841 ggtggaacat ggtaagcccg tatcgctgcc tcgtgaggca ggttaaccgc aagggatact
    15901 gttggcggtg cgggtaaaag aaggtggaaa aagcgaatgc cagtctgtaa tggaccggat
    15961 aagggttgag cccggcaaca ttaccctacg cgaaagcggg cagacttcca ctgggtcttt
    16021 cattacgaga gagtttgagt aatcttccag aaaggaaaag cagatgactg agcaggctac
    16081 aacctgtaaa ggtgcgtcct tacttaacgg tgactcctgg cacagtatca actggcgtca
    16141 gtgctatcgg gaagtgcgga gactgcaagc gcgtatcgta aaggcaacac gagaaggtaa
    16201 acatggcaag gtcaaatcct tgcaatggat actgactcac tcgtttagtg gcagagcagt
    16261 tgctgtcaga cgagttacag ataatagcgg gaaacgtact cctggggttg atggtcagac
    16321 atggtcttcc ccggaggtta aatttcttgc tattaacctg ctgaaacggc gtggttacaa
    16381 accacagcca cttaagcggg tttatatacc taagtcaaat gggaagtctc gtccccttgg
    16441 gatacccact atgaaggatc gggcgatgca ggcgttgtat ttgctggctc ttgagcccgt
    16501 tgctgaagtc actgctgacc agagatcctt cggctttcgg actggaaggt ctactgcaga
    16561 tgcgatagca cagtgcttct gtgttcttgc gcagaaaaca tccgctgaat gggtgcttga
    16621 gggggatata cggggatgct ttgacaatat atctcatcaa tggctaatcg ataacacctc
    16681 aacagacaga caaatcctga caaagtggct taaagcgggt tacagggaaa aggggcagtt
    16741 attccctgta aattcaggaa ctcctcaggg gggaataatt tctcctgtac tggctaacat
    16801 tgctctggat ggactggagg cgcttcttgc ttcggagttt aaaaagagaa cagtcaaggg
    16861 acgattggtt aacccgaaag ttaactatgt gagatatgct gatgatttta ttatcactgg
    16921 agagtcaaaa gaacttcttg agtctcaggt attacccgtc gtcaggcggt ttatggctga
    16981 aagaggatta atgctttctc cggaaaagac gaaaatcaca catatcgagg aaggatttga
    17041 ttttcttgga cagaacattc gcaaatatgg cggtaagatg ctgatcaaac cctctaaagc
    17101 caatgtcagt tcctttttaa agaaaatacg cgctgtaatc aaaggcaaca aagctatgga
    17161 tcagcttacg ttgatccgca tgctgaaccc aatgatcaaa gggtgggcgg cttaccatca
    17221 acacattgtg gccaaggtcg catttaacaa agttgacaat gagatctggc tggctttatg
    17281 gcgttgggct gttcggcgtc atcccaataa gggaaagaag tggattcgga aaagatattt
    17341 ccatcaacaa ggtgcccgaa actggtcttt ctccacggct accggagaac tccttgccaa
    17401 cggtaagcct cagtatgcaa atctgaggaa agcaatcgat acgcctatca accggtttaa
    17461 accgattaaa attgcagcta atccgttcga tcctcaatgg gagatgtact ttgaggagcg
    17521 ttgtgcagac aaaatgcggc acaaactgca aggacggaaa aagctcattc aaatctggtt
    17581 tgagcagcgg ggccgatgcc ccatatgtga tgagaggatt acatctgatt cccagtggca
    17641 ggttcatcac atcatacggc gagtcgatgg agggagcaac tgcctctcca atttgattat
    17701 gctgcatccg atgtgtcaca cacttgttca tgcgaaaggt atacatgtag tgaaaccggc
    17761 tcatgagagt gggcttagaa aggcttgagc cgtatgccgg gaaactggca cgtacggttc
    17821 ttagggggcg gtgatgcagt aatgcatcac tgctacccga tcgtatgtat ttttctggtg
    17881 aaccggcaca gatcgctgag attaaacgac tggccagcgg cgcagtcaca ccgttttatc
    17941 gccgcgccac aaatgaaggt attcagctgt ttctggccgg aagtgccgga cttctgcaga
    18001 ccactgaaga tgtgcagttt gaaccgtgcc ccggactgac ggctgccgga cgtggtgttg
    18061 tatcgccgga gaatatcgcg ttcacccgct ggctgacaca cctgcagaac ggtgtgctac
    18121 tggatgaaca aaactgcctg atgctgcatg aactctggct gcagagcggt actggacagc
    18181 gtcgctggga aggattaccg gacgatgtca gggataccat caccgcactt ttcaccgcaa
    18241 aaagaggtga ctggtgtggc ttctggagta acgaggatgt atcggtgtgg tggaaccgtc
    18301 tgtgtgacaa cgtactgccg gaaaaaacca tgccgtttga cctgctgacg gttctgccca
    18361 cccgcctgga tgttgaagtg aatggcttta acggtggtgt tctgaacggt gttccttctg
    18421 catatcactg gtatacggaa cagtatggcg tgaagtggcc tgtggggtat gaggtgaata
    18481 tcagtagtca gggagacaac ttcattcagg ttgatttcga cacgccgtgg tgtcagccgg
    18541 aaagcgacgt tattgcagaa ttaagccgcc gtttcagctg cacgctggag cactggtatg
    18601 ccgaacaggg ctgtgatttc tgtggctggc agttgtatga gcgcggagag ctcgttgatg
    18661 tgctgtgggg ggaactggaa tggtcttccc cgacagatga cgatgagctg ccggaagtca
    18721 ccggacctgc gtggatagtc gacaatgtgg cgcattatgg cggatgaagt atgacggaaa
    18781 cgggcgggaa accgcccgtt tcttttccga caaaggatgt cgccgtgctc ctctttttac
    18841 tgcgccggct gacgcggcgc ggcataaacg ctgcctgtgg tcagtgtccc gcgtccggcg
    18901 ggcacgggac gggcgctttt accgctcccg gctggcgtcg gtgacagtgt acgccagccc
    18961 gtcgcccttt tctgatgaac gcccttcaag ccgctttcgc ggcataacct tgccgtcaga
    19021 aagacgacgg ctgcggtatt ccacggtcgg cctgacccgt taccggacgc ggtgaacagc
    19081 ccacaggcag cggggaacgg gcaccacagg ggtgccctcc cggtgccctc tgtaagagaa
    19141 ggagtttcgt atgtcccgtt ttgtcctcgg taactgcatc gatgttatgg cccgtattcc
    19201 tgataacgcc attgatttca tcctcaccga cccgccatat ctcgtcggtt tccgtgaccg
    19261 ttccgggcgc accatcgccg gcgataaaac cgatgagtgg ctgcaaccgg cctgtaatga
    19321 aatgttccgc gtgctgaaaa aagacgcgtt aatggtgagc ttctacggct ggaaccgcgt
    19381 cgatcgcttt atggccgcct ggaaaaatgc gggattcagc gttgttggtc acttggtctt
    19441 caccaaaaac tacacatcga aggccgcata tgtgggctat cgccacgaat gcggctacat
    19501 cctggcaaaa ggccgtccac gtctgccaca aaacccgctg ccggacgtgc tgggctggaa
    19561 atattcgggc aatcgccatc acccgacgga aaagcccgtt accagcctgc aaccgctgat
    19621 tgagagcttc acacacccga acgcaattgt gctggacccg tttgcaggca gcggctcaac
    19681 ctgcgtcgcc gccctccagt ccggacgccg gtatatcggt atcgagctgc ttgagcagta
    19741 tcaccgtgcc gggcagcaac gccttgccgc cgtgcaacgg gccatgcagc agggggccgc
    19801 gaatgatgac tggtttatgc cggaggctgc gtaaatgaac tatgcaggac acgaaaaact
    19861 gcgcgccgaa gtggcggagg tggccaatgc catgtgcgac ctgcgtacaa ccatgaatga
    19921 gatggagcag cggtacagct ttaatgccga caccctgccg gaacgtctgg tgcgtcagac
    19981 gctgtttcgc gcaaaccgcc tcctgatgga ggcatatacc gaaattctgg aactggatgc
    20041 gtgctttaaa gattgagaag gagacgagaa tgtacggaac atgcgaaacg ctatgccggg
    20101 agctggcagt aaagtatccg ggagacatgc cgctgatgct ggttatctgg tccccggaag
    20161 agattcaggc cctcgctgac ggaatggata tttccctgtc cgatcatgaa atcagaaccg
    20221 tcctggcgcg cctggaggac atcccggaag accagcggac tgaatccggt atttcttccg
    20281 gcgtggcgat ggagatcatc aataacgtga gcgaaaaccg ccaggtgacc gtccctgctg
    20341 aactgctggc gtccctgatt cagaccgctg aacaggcatt gtggaaacgt gaatgggccg
    20401 cccgggatca tggcctcgcc gtcccggaat gcgtcacccg ccgtcaggcg gtgattaatc
    20461 aggcccgcac cctgctgaaa aacaacagac acgaaaacga ctgatgttat cgccgcctcc
    20521 gggcggcgat tcagggagac catgaatcat gaacgaaacg ctgaatgcac tgatttgtcg
    20581 ccacgcccgt aacctgctgc tggcgcaggg ctggccggaa gagacggatg ttgaccagcg
    20641 aaatccgaac catccgggct ggatcagcat ttacgtgcgg ctggatgcgc cccggctggc
    20701 gacgttactt atcaaccgtc acggcggcgt actgccgccg cacctggcct ctgccattca
    20761 gaaactgacc ggaaccgggg cggaacttgt tctgtccggc agtcagtggc agtcgctgcc
    20821 ggtacttccg gcagacggaa cgcaggtgtc tttcccgtat gccggtgagt ggctggcaga
    20881 agacgaaatc agggctgtac ttgcggcggt gcgcgatgca atacgaagca tctgttacca
    20941 ggtggcagac gatgcgcggc gtatccgggc ggcgctgacc accaccggtc agacgttgct
    21001 gacccgccag acgcgccgct ttcgtctggt tgtgaaggaa agcgatcacc cctgctggct
    21061 cgatgaagat gacgaaaacc tgcccgtggt gctcgatgcc atcgtgaacc ggggagcacg
    21121 tttttcgtcg gtggaaattt acctggtcag cgattgtatt gagcatatcc tgtccagtgg
    21181 gctggcctgc gatgtgctgc gtataccgga tgaaccgccc cgccgctggt ttgaccgtga
    21241 tgttctgcgg gaggtggtcc gggaagcccg ggccgaaatc cgcagcatgg cggatgccct
    21301 ggcaaaaatc aggggctgat gatggcgggg aaaatccccg ctttttttct gccggtcccc
    21361 ggacagagca caaaggaagt cgccgccggc tccgctttac ccggccatgc aggcatggcc
    21421 ttgtgggttt tcagttatgt ggccgccgtg tcgtgtgcgg gcggtgccgt gcctccatct
    21481 tagccgggct ggcggggatg caagggtacg cttcgccgct gcggtcaccc ggtccctcct
    21541 tcccgtctgc cgtgattttc cggtcgttca cccgctcagg tttcgcggtc tcaccctcca
    21601 actcccgtca gccgtgtgcg tcaggctgcg gcttcccttg cgccctgtgc atccccgcct
    21661 tatcgcccgg cttttatgga ggcacggcac cgcccggtgt cgcggaatat gtgaataacg
    21721 gaggaaaaca tcatgcaata tgcgaaacct gtcactctga acgttgaaga gtgcgaccgt
    21781 ctctcctttc tgccttacct gtttggcaac gattttctgt atgccgaggc gtatgtgtac
    21841 gcgctggcgc aaaaaatgat gccggaatat cagggaggct tctggcactt catccgcctg
    21901 ccggacggtg gcggttacat gatgccggat ggcgaccgtt ttcatctggt caacggtgaa
    21961 aactggtttg accgcacggt cagcgccgat gccgcaggca tcattctgac ttcccttgtg
    22021 attaaccgcc agttgtggct gtaccacgac agcggggatg taggactgac ccagctttac
    22081 cggatgcgcg atgcgcagtt gtggcgtcac atcgaatttc accccgaatg caacgcgatt
    22141 tacgcggcac tggactgatt aacggacggg gcggcaatgc cgcccctgta aaggagacga
    22201 gaacatgtac tgtactgtta aagaaatcat ccgcgatgta ctggatacag acgtgccgga
    22261 cagtgaatgc gtttttgccg tggtgctgac ccgtggggat gtgcgccaca tagcccagga
    22321 ctggaacctg tcagacgatg agctggaaac cgtcatgcag cggctggacg atgcctttga
    22381 atatggtgcg gatgtcagca ttgttcacga cgttgttcgt gagctgatgg aagaaaagcg
    22441 tgccagccgt caggtgacag ttccggcggt gatgctggaa aaagtgatgg cgctggcagg
    22501 cagtgaaatg aagcgcctgt atgccgtcgg gagtgagaac gggggcgacg gtgatgcgtt
    22561 cgtcagggag gaacgcgaag caatggacgt tgtgttacag gcgctggacg gggagcacat
    22621 gtcatgaata tcagcacaga aacccgcgaa attctgcgta attacagggc cgtgattaat
    22681 gcgcggcgtc gtgaaatggg gcagaaaccg ctcaccactg cgcagattgt tgatgaaatc
    22741 tgcgattttg tggcgaatca gcaggcggtt ttcctcggtg gtcactatat ccttcagggc
    22801 agcagaaaca ggtgatacag ggcaggcggt ggaggccgcc tgtcagcccg cgcgaaaacc
    22861 cctggcaggc tgacgcctgc cggaaatgcg gggtgcagcg cctgcggcgc tgccggcccg
    22921 gctttccggt cagaaggtag tgtatggctg tatgcaggga acaggtgtga tattccgtga
    22981 cactgtcacc gtttctggct gtcttttatt gcgcccactt cggctcccgg ctgcctggcg
    23041 gcatccggtc gtcaggcccg actccgacag gcagctgctg gcgcacccgc cagtctccgc
    23101 cgaacccgct ggcgcggttt cgggctgccg ctacggtccg atgcgtgaaa acccggcttg
    23161 cagcgaccgt tccggtcgcg ccgcccctgt cgcgccgtga cctgcgctcc ctgcacacct
    23221 gacggtgtgt gaggaaactg cgcctgatgg cgcagcagtc ttccctcctg ccgccacccc
    23281 tgcggcctgt ttcccgctgt ggactttccg ggggggcaga cgtaaccccc tcccgttcca
    23341 tctgtccagg gtgagatgca ccggccacaa aatttttaac catgaacccg gctaaaaatt
    23401 ttgcgtccgc ccccggacag ctgtcccgtg aggcggtaaa cgggccccca tcggaaaggt
    23461 ccactgcggg gagaaacccc tgtgaagtgg acggcaggag gagctttcgc tcctctgaag
    23521 gggcacatga acgtcccgtc tccggtatcc ggtttatctc acatctttca gtcaggaggt
    23581 tcttatgagt gcacgtggta tcaacaaggt catcctcgtc gggcgtctgg gcaatgatcc
    23641 ggaagtccgt tacatcccca acgggggcgc agtggcaaac ctgcaggtgg ccacatcaga
    23701 aagctggcgt gacaaacaga cgggggagat gcgggagcag acggaatggc accgcgtggt
    23761 gctgttcggc aagctcgcgg aagtggcagg tgaatatctg cgcaagggtg cgcaggtcta
    23821 catcgaaggt cagcttcgca cccgtagctg ggaagataac ggtatcaccc gttacgtcac
    23881 tgaaattctt gttaagacca cgggcaccat gcagatgctg gggagtgcac cacagcagaa
    23941 cgctcaggcg caaccgaagc ctcagcagaa tgggcagcca cagagtgctg acgcgacgaa
    24001 aaaaggtggc gcgaaaacga aaggccgtgg acgtaaggcc gcgcagcccg agcctcagcc
    24061 gcaaccgccg gagggggagg attacgggtt ttcagacgat atcccgttct gactggcctg
    24121 actgtgataa ccgccccgcc ctgcgcgggg catcaccgga gatatgagga tgagcgaata
    24181 tttcagaata cttcaggggc tgcctgacgg ctcctttacc cgcgaacagg cggaagccgt
    24241 tgccgcacag taccggaacg tctttatcga gcggggaaca ttttcgcttg gttgtccgca
    24301 ataacggtgc cctggtctgg cgtacctgga attttgagga cggtgccggg cactggatga
    24361 atcatgccat cagggatttc gggattctta agtaagagag gtgccggacg cgcgaacgtc
    24421 cggcaggaca taagcaatta taaggggatg attatgcctg taacgaagtg tgaaccagaa
    24481 accacccgca aagcaagccg taaatctgta aaaactcagg aaaccgtcct gtctgccctg
    24541 ctggcgcaga cggcggaagt gagcgtgccg ctggcctcac tgattaagtc accgctgaat
    24601 gtgcgcacgg tgccgtattc tgcggagtcc gtcagcgaac tggcggactc cattaaaggt
    24661 gtcggcctgt tacagaatct ggttgttcat gccctgccag gtgaccgcca cggtgtcgcc
    24721 gcaggtggtc gccgactggc agcactcaac atgctggcag agcgtggcat cattccggct
    24781 gactggcctg tacgcgtgaa ggtcattccg caggagctgg cgactgccgc atcgatgacc
    24841 gagaacggtc agcgtcggga tatgcaccct gccgaacaga ttgccggatt ccgcgcaatg
    24901 gcgcaggaag gcaaaacacc tgcacaaatc ggtgatttgc tgggctattc accccgccac
    24961 gttcagcgaa tgctgaaact ggctgacctt gcgcctgtca tcctcgatgc gctggcagaa
    25021 gaccgcatca ccaccgaaca ctgtcaggcg ctggcgctgg agaacgacac cgcgcgtcag
    25081 gtgcaggtgt ttgaagccgc ctgtcagtcg ggatggggcg gaaaaccgga agtacagacc
    25141 attcgtcgtc tggtgaccga aagtgaagtg gcggtggcgg ggaacagtaa attccgcttc
    25201 gtgggggctg atgccttctc gccagatgaa ctgcgcactg acctgttcag cgacgacgag
    25261 ggtggctatg tggactgcgt tgcgctcgat gctgccctgc tggaaaaact ccaggctgtt
    25321 gctgaacacc ttcgggaagc cgaaggctgg gaatggtgcg ccgggcgcat ggagcctgtc
    25381 ggtgagtgcc gtgaggatgc cggaacatac cgctgtctgc cggagccgga agcggtgctg
    25441 acggaggcgg aagaagaacg tctgaacgaa ctgatgacgc gttacgacgc gctggaaaac
    25501 cagtgtgagg aatctgacct gctggaagca gaaatgaagc tgatgcgctg catggcgaag
    25561 gtcagagcgt ggacgccgga gatgcgtgcc ggaagcggtg tggtggtgtc ctggcgttat
    25621 ggcaacgtgt gtgtccagcg tggtgtgcag ttgcgcagcg aggatgacgt ggctgacgac
    25681 gctgaccgca cggaacagat gcaggagaaa gcgtcagtgg aggaaatcag tctgccactg
    25741 ctgacgaaaa tgtcttccga acgcacgctg gcagtccagg cggcactcat gcagcaaccg
    25801 gacaaatctc tggcactgct ggcatggacg ctctgcctga atgtgtttgg cagcggagcg
    25861 tacagtaaac cagcacaaat cagcctggaa tgtaaacatt attcgctgac cagcgatgcg
    25921 ccgtcaggga aggaaggtgc cgcgttcatg gtgatgatgg cagaaaaagc ccgtcttgcg
    25981 gccctgttac cggagggatg gtcacgggac atgacgacgt tcctgtcact cagtcaggag
    26041 gtgctgttat ccctgctcag tttctgcacc gcgtgcagca tccacggcgt tcagacccgc
    26101 gagtgtggtc acacgtcacg cagcccgctg gacacgctgg agagcgccat cggttttcac
    26161 atgcgcgact ggtggcagcc gacaaaagca aacttcttcg gacacctgaa aaagccgcag
    26221 attatcgcag ccctgaatga ggcaggactg tccggtgccg cacgggacgc ggagaagatg
    26281 aagaaaggcg atgcggctga acatgcagag ttccatatga aagacaaccg ctgggtgcct
    26341 ggctggatgt gtgcaccacg tccacagatg gatgccactg aacacaccac taacctggct
    26401 gatgccgcct gatgaacaac cacaccgccc cgccggagac ggggcggcag caagggagat
    26461 accgtgatga aaactgaact gaccctgaat gccctgcagt ccatgaacgc acaggaatat
    26521 gaagatatcc gtgctgcggg aagcgatatg cgccgtaatc tcactcacga ggtgatgcgt
    26581 gaagtggacg caccggttaa ctggatgatg aatggcgagt atggcagtga gttcgggggc
    26641 tttttccccg tccaggttcg tttcacgcca gcccacgaac gttttcacct ggcattatgt
    26701 tcgccgggag acgtctctca actctggatg ctggttctgg tgaatggtgg tggacagcct
    26761 ttcgccgtcg ttcaggtgca acatatcttc acgcctgtcg cgatcagtca cacgctggcg
    26821 ctcgccgcga cacttgatgc gcagggatac agtgttaacg acatcattca tattttgatg
    26881 gcagaaggag gtcaggcatg agcgcacgtt cacgggcact gatccccctc agcgcagaac
    26941 aacaggccgc catgcaggct gtggctgtca ccgaacaacg tcgtcgtcag ggacgcacac
    27001 tttcagcatg gccttatgcc agcgctttct ttcgctgcct gaatggcagt cgccggattt
    27061 cgctgaccga tctccgcttt tttgcccctg cgctgacgaa ggaggaattt catggcaacc
    27121 gtctcctgtg gctggctgcc gtggataaac tgattgaaag ttttggtgaa gtctgtgttc
    27181 ttcccctgcc atccgatgcg gggcatcgtc tgttcccgtc cgttcctttt cgtgaaggtg
    27241 agcgtcgtcg tcagaaaacc acgctgacag agcagaaata cagccgccag cgggaacgtg
    27301 aggcagaacg acgggaactg gaataccaga catgttttgc tcaggcgcag attgaccttg
    27361 cgtttcatac tcccgccacg gtcggaagct ggttgtcccg ctggtctggt gttgttgagg
    27421 agcatgatct ggaaacgatt ttctgggggt ggtgcgggcg ttttccatca ctgtcatcat
    27481 ttgaccggtt tttctggcag gaggaaccac tctggcggct gatttttgaa gccggtgagg
    27541 ccggtcgtgg tgcaccggta caggtacgtg cacttgagca gtggatgatc ccgaacaagc
    27601 tggagaacgc aatatgatga aatcagacga acaatatcag gttcccgtgt ggatgcgacc
    27661 tctgttgccg ttgctctgca ataccggtgg taatgatccg gaggaactgc tgaatgatac
    27721 agaaaccacc gccagtgcga atattgtccg ttatgtactg atagttgcag tgcggtcgca
    27781 gattgatctg ctgcagctac tgtacaggaa aggactgttg cgcacagaga taccaggtgg
    27841 cttttcaccg gaagaagcgc aggaactcct ggataatctg gtgcgcagcc atatcagcaa
    27901 ggcgctgtcg ggtgagcgaa tggcagcccg tgacagaaat gccgatctga cctggattcg
    27961 ccagcaactg gtcgatgccg cctggtttgt ccgtgccaca ctggaagcgc atgggatggg
    28021 cgtcggaaat gagagtccct ctgctccgcc ggagacaatg ccggacatac agacacggga
    28081 actggttatg ttgatcaagc gactggcatc atcgctgaaa gcggtgaaac ccgacagtag
    28141 cgtggtgcgt gaagcgcagg actggctgtg cgacagaaaa cttgtggata tcacagatat
    28201 tctccggtga accagacatg tatattttac gcagggacac gccgatcatg atcgtttcat
    28261 atcaggggtg gtctggaggt catgcggcgt gtcctctgca ctcgccggaa taaggaagtc
    28321 gccggcggct ccgcttttac ccggccatgc ggggcatggc cttgtgggtt ttcagctctg
    28381 tggcctcagc gttgtgtgcg ggctgtgccg tgcctccatc ttagccgggc tggcagggat
    28441 gcaagggtac gcttcgccgc tgcggtcacc cggtccctcc ttcccgtctg ccgtgatttt
    28501 ccggtcgttc acccgctcag atttcgcggt ctcaccctcc aactcccgcc agccgtgtgc
    28561 gtcaggctgc ggcttccctt gcgccctgtg catc
//
LOCUS       DAZFZC010000066        28584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFZC010000066 DAZFZC010000000
VERSION     DAZFZC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.066"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1392)
                     /gene="ccp"
                     /locus_tag="ACHKXP_003175"
     CDS             complement(<1..1392)
                     /gene="ccp"
                     /locus_tag="ACHKXP_003175"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO7648127.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKTQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDK"
     gene            1796..3445
                     /locus_tag="ACHKXP_003176"
     CDS             1796..3445
                     /locus_tag="ACHKXP_003176"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7648128.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3496..4098)
                     /locus_tag="ACHKXP_003177"
     CDS             complement(3496..4098)
                     /locus_tag="ACHKXP_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO7648129.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4618..5517
                     /locus_tag="ACHKXP_003178"
     CDS             4618..5517
                     /locus_tag="ACHKXP_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7648130.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLTAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5566..6579
                     /gene="yhjD"
                     /locus_tag="ACHKXP_003179"
     CDS             5566..6579
                     /gene="yhjD"
                     /locus_tag="ACHKXP_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO7648131.1"
                     /translation="MTQENEIKRPIQDLEHEPIKPLDNSEKGSKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            6990..8312
                     /locus_tag="ACHKXP_003180"
     CDS             6990..8312
                     /locus_tag="ACHKXP_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648132.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8494..10554)
                     /locus_tag="ACHKXP_003181"
     CDS             complement(8494..10554)
                     /locus_tag="ACHKXP_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7648133.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10624..11391)
                     /gene="pdeH"
                     /locus_tag="ACHKXP_003182"
     CDS             complement(10624..11391)
                     /gene="pdeH"
                     /locus_tag="ACHKXP_003182"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO7648134.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11623..12552
                     /gene="kdgK"
                     /locus_tag="ACHKXP_003183"
     CDS             11623..12552
                     /gene="kdgK"
                     /locus_tag="ACHKXP_003183"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO7648135.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12648..14144)
                     /locus_tag="ACHKXP_003184"
     CDS             complement(12648..14144)
                     /locus_tag="ACHKXP_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7648136.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAVWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14365..15651)
                     /gene="dctA"
                     /locus_tag="ACHKXP_003185"
     CDS             complement(14365..15651)
                     /gene="dctA"
                     /locus_tag="ACHKXP_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO7648137.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15834..17822)
                     /gene="hmsP"
                     /locus_tag="ACHKXP_003186"
     CDS             complement(15834..17822)
                     /gene="hmsP"
                     /locus_tag="ACHKXP_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO7648138.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPGDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17904..21377)
                     /gene="bcsC"
                     /locus_tag="ACHKXP_003187"
     CDS             complement(17904..21377)
                     /gene="bcsC"
                     /locus_tag="ACHKXP_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO7648139.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21359..22465)
                     /gene="bcsZ"
                     /locus_tag="ACHKXP_003188"
     CDS             complement(21359..22465)
                     /gene="bcsZ"
                     /locus_tag="ACHKXP_003188"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO7648140.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKEGLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22472..24811)
                     /gene="bcsB"
                     /locus_tag="ACHKXP_003189"
     CDS             complement(22472..24811)
                     /gene="bcsB"
                     /locus_tag="ACHKXP_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO7648141.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24822..27440)
                     /gene="bcsA"
                     /locus_tag="ACHKXP_003190"
     CDS             complement(24822..27440)
                     /gene="bcsA"
                     /locus_tag="ACHKXP_003190"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO7648142.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27437..28189)
                     /gene="bcsQ"
                     /locus_tag="ACHKXP_003191"
     CDS             complement(27437..28189)
                     /gene="bcsQ"
                     /locus_tag="ACHKXP_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO7648143.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28201..28389)
                     /gene="bcsR"
                     /locus_tag="ACHKXP_003192"
     CDS             complement(28201..28389)
                     /gene="bcsR"
                     /locus_tag="ACHKXP_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO7648144.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         6726 a         7901 c         7197 g         6760 t
ORIGIN      
        1 tttatcctgc atatacggcg tgtacacccc gttcagactg tgcaggaaag ctacgatatc
       61 atccacatcc tcctgcggca gctctttgcc tacctgatag cgcagcatca gtttcaccgc
      121 cccgtccagc gtcggcacgt caccgcgatg gaagtacggt gccgttaaag caacgttacg
      181 taaaccgggt actttctgac gcaatttatc acgctcttct ttagtcacat tcatacgacc
      241 aatatccgcc gccgtaattt ccccaaagtt aaagtctttt ttcagcccca acggttcaaa
      301 ggaacgtccg ccgagaataa taccaccatg acaagttgca catttattat ctttaaataa
      361 ttgatagcct tttttctgtt gcgccgtcag cgcattttca tctccgcgca accatttatc
      421 aaatggggaa tccggcgtaa ttaatgtttt ctcaaattca gcaatggcat cagtaatatt
      481 ttcgccactg aaaccttgcg gatagacttc gaggaactgc gttttaagct gcggatcttt
      541 ttccagctta gcaataattt cgtcccagga tttcgacgcc atttcaatcg ggttcaacgg
      601 cggtccacca gcctgatcct gcaatgttgc cgcacgacca tcccagaact gctcaacgtt
      661 aaatactgag ttaaataccg tcggcgcgtt aatcggccca actgcgccac caacaccaat
      721 cgatgttttt ctgccatcga cgccccccgc attcaacgca tggcaatgag cgcatgaaat
      781 ggtgctatca gccgataaac ggggatcgtg atacagcgca aaacccaacg ccactttttg
      841 cgcatcggta ggcagttttt gcgggatggg ctgcaccggt tcattgcgat gttccggagc
      901 agtatcattg ctggcgtaat attccgcgcg ctgttttgca atccaggcca gtatttccgc
      961 ccgctcttca tcactcacct tacccgccca gtgtagcgcg gtataacgcg ttggtggcat
     1021 agtttcatac tgcatcaccc attcaatctt attcaaatcg ctttgcgaaa cgggtttatc
     1081 agccagcagt gccgcacgca cggcctcaag gttaaaagat ttatatccaa gcttaatgtc
     1141 gtaatccatc aactgtttcg cgccaggaat ataataatag gcgggtaatt ctgccgaagg
     1201 cgtgtggcaa tagtcgcatc ctttttcgcg gagaaagcct aaaaccttat tattttcact
     1261 gacagcagat gcctgaacat cggcctgttt actgcgttta ttatcgtggt accacacata
     1321 accagataac cctaaatagc aaatcgcgac gccagccagg ccgatcgcgg taatacgtga
     1381 gaccattttc attattcttc cctcacggtg gtgagttatt gtgacccaga aaattttcca
     1441 gaatgctgca aacgtctctg cgagacaaag ccatcataaa aaacagggag atacgctttt
     1501 tgataggaat caatcaatta catgaatata aactatcaat aagatagcct gtgcctatta
     1561 cccagatcag gaatggcata tcatgattgc ggcgagagca ggattgaatg ttctgaaaat
     1621 gaaaaacaac accaaatcgg tatttttata aattccattg atattagtgc gttatatttt
     1681 ttgacaggtt aataaacgtg atccaccgca cgctttgtcg cccaccaggc ggagcgaatg
     1741 actaccctta aagaaaagcc aataattagc gacgaatttc ggaggttgga tccttatgct
     1801 caatcagaaa attcaaaacc ctaatccaga cgaactgatg atcgaagtcg atctctgcta
     1861 tgagctggac ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga
     1921 gatgattgaa gggctgcctg cctctgatgc gctgacgcct gccgatcgct atctcgaact
     1981 gttcgagcat gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc
     2041 acctaaaatg gacccgctgg atatcttaat ccgctaccgt aaagtgcgcc gtcatcgtga
     2101 ttttgacttg cgcaagtttg ttgaaaacca cttctggctg ccggaggtct actccagcga
     2161 gtatgtatcg gacccgcaaa attccctgaa agagcatatc gaccagctgt ggccggtgct
     2221 aacccgcgaa ccacaggatc acattccgtg gtcttctctg ctggcgctgc cgcagtcata
     2281 tattgtcccg ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct
     2341 ggggctggcg gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg
     2401 gatgatcgaa aactacggtc acatccccaa cggcaaccgc acctattatt tgagccgctc
     2461 gcaaccaccg gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc
     2521 gcgccgctat ctcgaccacc ttaaaatgga atatgccttc tggatggacg gtgcagaatc
     2581 gttaatccct aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa
     2641 ccgttactgg gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac
     2701 cgcgaaacat tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggggcggc
     2761 ctccggttgg gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg
     2821 taccacccag ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat
     2881 cgccaacatc tcggcgctga aaggcgagaa agagacagaa gcactgttcc gccagaaagc
     2941 cagtgcccgt cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg
     3001 cgattacgac tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccact
     3061 ctatgtcggt atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tgcgcagtcg
     3121 gttactgaca cctggcggga ttctggcaag cgagtacgaa accggtgaac agtgggataa
     3181 acccaacggc tgggcaccgt tacaatggat ggcgattcag ggatttaaaa tgtacggcga
     3241 tgaccttctg ggtgatgaaa tcgcgcgaag ctggctgaag acggtgaatc agttctatct
     3301 ggaacagcac aaactgatcg aaaaatacca tattgccgat ggtgttcccc gcgaaggcgg
     3361 cggtggcgag tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt
     3421 aattggtttg tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct
     3481 gtaaaattat cctcttcagg aggagatatt taacatcatt gccgcctggg tgcgattttt
     3541 cacttccaga cggcgataca gggattccag atgcgctttt accgttccgg tactgatatt
     3601 cagcgctctg ccgatctcct tatttgattc gcccgccgct aacatggtta aaatctcccg
     3661 ctggcgggcg cttaacgatt tgagatcttt aatgtccttt tccggcgtcg tccgccagtc
     3721 tccaggcaga aacatcatcc ccatcgccgc actatttacc gccaacgcaa atgtctcgac
     3781 ggttgaatca cgaggcacaa tggccagcac attaaaatgg ataacttcct gtaaccaccg
     3841 tttattgcaa tccgtcgccg taattaacac cttaacctca ggaaattgca ccacggtttt
     3901 ttgcagcaac cagtagcaaa actcaccatc ctgatcgcca tcgagcataa ctaaggcttc
     3961 agggtaactt tccagctttt gccataactc gtctgcctga ctggccccct gaatactcac
     4021 tcctggaata cgctgctgta aactgatttt cattccatga ataaatattg actgcctgtc
     4081 aaacatgact atttgcataa ctgaatctcc acctgaatac gttaaaaaga cttaagtagt
     4141 ggaagggtat tacccgcgag aaaaaataag aattcgccat ttggcggtgg ccattctaca
     4201 gagatgacgt gtagaaaata gttaccgata taaatagtta cagctaaacg cctgaaatta
     4261 catgtcgagg gcactattta aaacaatttt gaggatttcc ttatattggt ggttagtacg
     4321 catgcaatta aaaatgaaat tccgcgacca caagccaaaa taacaaacgg caaggagaca
     4381 aaaataagca caaatagcca acacgtcctc tgttcacttt aaagggaatc gctgaaaaat
     4441 acgctctgtt taaggggatt cacctttctc agaaagctat tccgcccttt tcctgctgag
     4501 aaatcgccac attcggcatg acaacattgt gaaacccggc attagatgtt agaaaaaaca
     4561 ataaacaatg cgatatgcgc gttactgttt cgctgtgaac aacaatcggt caaagaaatg
     4621 gataaaattc acgcaatgca gttgttcatc aaagtcgcgg agctggaaag tttttcccgc
     4681 gcggcggatt tctttgcttt gccaaaagga agtgtttcgc gccagataca ggcactggaa
     4741 catcaacttg gcacccagct tctccagcgc accacgcgac gggtcaaact cacgccagaa
     4801 ggcatgacct attatcaacg agcaaaagat gtgttgagta atctcagcga actggacggt
     4861 ctgtttcaac aggatgccac cagtatcagc ggtaaattac gcatcgacat cccgccagga
     4921 atcgcgaaaa gcctgttact gccgcgcctg tcggaatttc tctatcagca tccgggaatt
     4981 gagctggaac tgagtagcca tgaccgtccg gtagatattc ttcatgatgg ttttgattgc
     5041 gtgatacgca ctggcgcgtt accggaagat ggcgttatcg cccgtcccct cggcaaactg
     5101 accatggtca actgtgccag tccgcactat ctgacgcgct ttggttatcc gcaaagcccc
     5161 gatgatctga cttcacacgc aatagtgcgt tatacaccgc acctgggtgt acatccgtta
     5221 ggttttgagg ttgccagcgt taatggcgtc cagtggttta agtctggcgg catgttgacg
     5281 gtaaacagta gcgaaaacta tctcaccgcc ggtcttgccg gtctggggat tattcagatc
     5341 ccgcgcattg ccgtgcgcga agccctgcgt gccgggcggc ttattgaagt attacctggc
     5401 taccgtgccg agccgctctc cctttcgctg gtttatccgc agcgtcggga gctttcccgg
     5461 cgtgtaaacc tgtttatgca gtggctggct ggcgtaatga aagagtacct ggactgaccg
     5521 actatacttt ttaagaatga ccacgacaga aggacaaaag agcggatgac gcaggaaaac
     5581 gagatcaaac gtcccatcca ggatctggag cacgagccga ttaagccgtt ggataatagc
     5641 gagaaaggca gcaaagttag ccaggcgctg gaaaccgtca ccaccactgc cgaaaaagtc
     5701 caacgccaac cggttattgc gcacctgatt cgcgcgacag aacgctttaa cgatcggctg
     5761 ggtaaccagt ttggtgcggc tatcacctat ttctcatttt tgtcgatgat accaattttg
     5821 atggtgtcgt ttgccgcagg gggctttgtg ctggcctccc atccgatgtt gctacaggat
     5881 atcttcgaca aaattctgca aaacatcagc gatccgacgc tagccgccac gttgaaaaac
     5941 accatcaaca ccgccgttca gcagcgtacg actgtagggc ttgtcggcct ggcggtggca
     6001 ctttattccg gcatcaactg gatgggtaac ctgcgtgaag cgattcgtgc ccagtcgcgc
     6061 gatgtctggg aacgctcgcc gcaagatcag gagaagttct gggtaaaata tctgcgagat
     6121 tttatttcgc tgattggttt gttgattgcg ctgattgtga cgctttcgat cacctcggtt
     6181 gccggttcgg cgcagcaaat gattattagc gccctgcacc tgaacagcat tgagtggctg
     6241 aaaccgacgt ggcgattgat tggcctggcg atttccatct tcgccaacta tctgcttttc
     6301 ttctggatct tctggcggtt gccgcgccac cgtccgcgca aaaaagccct gattcgcgga
     6361 acatttctgg ctgctattgg ttttgaagtg attaaaatcg tgatgaccta caccctgcca
     6421 tcgttgatga aatccccctc tggcgcagca tttggttccg tgctggggct gatggcattt
     6481 ttctacttct tcgcccgttt gacgctgttt tgcgcggcgt ggattgccac cgccgaatat
     6541 aaagacgacc cgcgaatgcc ggggaaaacg cagccttaaa ataggccgga tgcggcgtaa
     6601 acgccttatc cggcatacga ttttattcgg cctggctccc cgtaggccgg ataagatgcg
     6661 ccagcatcgc atccggctat aatgcgcaca taacctcttg aaactcgtcc ccagagcctc
     6721 ttcagccatc tatttgggag caaacaattt cattccaact cataacccca gcatataaat
     6781 ccagttggta acttttattt aacctgaaac cagttttatc cactatttat aaaattatgt
     6841 gaagcatttc atagaagaaa aatcactggc ctaaacatta tccccttttt gcctgatttt
     6901 tgaccatttc cgcgatttgt tacacattga aatatcactt ttgctgtgcg taatatggct
     6961 attcgttagc caaaaaataa gaaaagatta tgcaagcaac agccacaaca ctcgaccacg
     7021 agcaagaata cacgccgatc aactcgcgta ataaagtcct tgtcgcctct ctcattggca
     7081 cagccattga gttcttcgac ttttacattt acgccactgc ggccgttatt gtgtttccgc
     7141 atatcttctt cccgcagggc gatcctacgg cagcaacgct acagtcgctc gccaccttcg
     7201 ccatcgcctt cgtcgcgcgc cccattggct ctgccgtttt tggtcatttt ggcgatcgcg
     7261 ttgggcgtaa agcgacgctg gtcgcctcgt tgctaacgat ggggatttcg accgtggtga
     7321 ttggtctgct gccgggctat gccacgattg gtattttcgc cccgctgctg ctggcgctgg
     7381 ctcgatttgg tcagggtctg ggcttaggcg gtgaatgggg cggcgcggcg ctgctggcga
     7441 ctgaaaacgc cccaccgcgc aaacgtgcac tgtatggctc ctttccgcag ctgggcgcac
     7501 cgattggctt cttctttgcc aatggcactt tcttgctgct ttcctggcta ctgaccgacg
     7561 agcagtttat gagctggggc tggcgtgtgc catttatctt ctcggcggtg ctggtcatta
     7621 tcggcctgta tgttcgcgtg tcgctgcatg agtcgccggt gtttgagaaa gtcgctaaag
     7681 cgaaaaaaca ggtgaagatc ccgctgggta cgctgctgac caaacatgtt cgcgtaaccg
     7741 tactgggtac gttcattatg ctggcaacct atacgctgtt ttacatcatg acggtctact
     7801 ctatgacctt tagtaccgcc gccgcgccag ttgggcttgg cctgccgcgt aacgaagtgt
     7861 tgtggatgtt gatgatggca gttattggtt ttggcgtgat ggtgccagtc gctggattac
     7921 tggctgatgc ctttggtcgc cgtaaaagca tggtaatcat caccacgctg atcatcctgt
     7981 tcgcgctgtt cgcctttaac ccactgctcg gttctggcaa cccgattctg gtttttgcct
     8041 tcctgctgct ggggttaagt ctgatgggtc tgaccttcgg gccaatgggt gcgctgttac
     8101 cagagctgtt tccgacagaa gtgcgttata ccggagcatc gttctcttac aacgtagcgt
     8161 cgattctcgg ggcttccgtt gcgccatata tcgcagcctg gttgcagact aactacgggc
     8221 taggtgcggt ggggttatat ctggcggcga tggctggctt gacgttaatc gccctgctgc
     8281 tgacccatga gacgcgacat cagtcgttgt aataagagtg aaaagcctga tgcgctacgc
     8341 ttatcaggcc tgaatttcca tacaatatat tgaattcgca aggatttgta ggccggataa
     8401 ggcgttcacg ccgcatccgg catgaacaaa gcacactttg ccaacaatct aaatctgttt
     8461 aattgccgga tgtcagacat ccggcaatta tcatcacttc ttcatctgcg acaaaatcgt
     8521 ccgacactga ttagcctccc cttcggaagg tgagatcagt gccagtaacg ccgccgccgg
     8581 tgttaccagc gttgccagtg ccgcagcaac agcaccacgg gcaatcagcg gtccggcttt
     8641 caccccagcc tgcggatttt taaacgtccc ccgcacatac agcggcgaac gcagtgtgat
     8701 aatccgaatt cctttactct ccggatcaat agtcaaatcc agctgttccg aagcaaaact
     8761 tgccgtgccg gtaacattaa tcaacgcgtt ctcagtatcg aaagcaaaaa tctgcgggcg
     8821 cgccacgccg ttggcaatat tcagattcgc cgccgcgcag ttcacccgca cctcatcatc
     8881 accaaatatc gcaccgacaa tgtagttgcc gacattcagc ccaacaatct ccatcaggtt
     8941 gcggctcacc agcccgtcat tcatcaacag tttcaggttg ccgttactgt tgcctaaaag
     9001 tgccgccacc gagttaccgc taccgcgtag ttccgcgtca ccgttcattt cccccagcgt
     9061 cttctgcatc agttccacat cgggcatcag ttctttcagt ttcagtcgac gagcctgaat
     9121 atctgcccgc ccctgcatcg gctttttatc gccttccaga tgaatattcg ccgcaatgct
     9181 gccgcccgcc atgccaaatt tcagcggttg caggcgcagg tcagcatttt tgaggatgat
     9241 atgagtagaa agatcgctaa tcggcaggct actgccatgc tcaatgcgcc gccctttgaa
     9301 gcgaacatcg gcatccataa cgtcccattt atcggtttcg aagcggtcat aaggcagcac
     9361 tttgcccgca ggctgaacgc ttttttcgcc cttcttctgt tcagaccgtt tcgacttttc
     9421 tgcccctttc ccggaatcaa cgccaatcaa cggtcccagg tccgccagcc gcaattgccg
     9481 cgactcgaca tcaccttcca gttttggtcg tggctttccg gtggtgtaga ccagagaacc
     9541 gtggatatcg ctatcaccaa ttcgcccatt aaaaccgcga taatcaaaga ccgacgattt
     9601 ttcagtgtcg attttcgcta ccagccgacc atccgtttca aacggcgggg tatcgggcag
     9661 cagaacgccc gtcagttcat agagatcacc cagtgaatcg ccagaaaatt taagccgtaa
     9721 atcgacaccg cccatcttca ttgggtcatt cacgacgcca tcaaaagcaa cacgggtgtt
     9781 accagagcgg aaatcagcct gtaccggaaa cggcgtccct tcgccacgca gcgccagcat
     9841 accgcctatt tttcccgtac ccgtgagcgg ttcaccgtta tatcgtccct gcgccttcag
     9901 gccaaaaacg taatcgccca ccttttcttt atccgcttta cctttcgatc cagtaacttc
     9961 gctgaacggc agcggcttgc ctaagggatc aacaaaaatc tccagatccg ctttgcttac
    10021 tttgtcatca atggcgatcc gcccttgatc gaaaagaata ttatccagcc gaaacgacca
    10081 tgccgacggc tttgcattcg cgtctttgtt atcatcgttg gcaagattaa acgtccagtt
    10141 attgttcttt tcagagaggc gaatcaggcg cgcgtcgggc ttttcgagct tgatccacgg
    10201 cagccagacc gttttggtca gcagcgccag cggggccagc gttgcctcta cgcgtggcaa
    10261 atgcaccatc gtgacttcgg gaatatccgg tggattgcca agaatgatgt cttccgcgtg
    10321 tacatggggc cacggcaccc agctgcgcca gccagtttct tgtttttgcc gctcccacac
    10381 cacgcccaga tcgccacgga tagcgaacgg acgattcaac tccgcagaga ctttctggtt
    10441 gatagtcggt ttgagtcgat tccagtcaaa agttgcaatc aaaatgatcg ccacgacaat
    10501 caacaacaag aaagcccctg aaatcgcagc ggttattttg cctgccttgc tcatcgttcg
    10561 cttcctcata ttcttcctgt gccagtccta aagatagtcc agccaggcgg aaaatgaggc
    10621 agcttatagc gccagaaccg ccgtattcag cgtttctatc ggtgccgggc gtgaaagaaa
    10681 ccagccttgt gcggcgaatg cgggcgagtt ctgaacatca cgccactctt ccggcgtttc
    10741 tacgccctcg acaatcaccc cgcgacaata gcgattcatt agatgtaaaa gctgagaaaa
    10801 gagtgtgcgt ccttccggcg actgacgcag catcacaaac agttctcgcg cgattttgat
    10861 gtagtcataa cgcacttcac ttagcgcaga gaaatttgcc atcccggtac caaaatcatc
    10921 cagccacagc gggccaaatt cacacatcga ggcaaaggtt gaatctttcg gcagacggat
    10981 atgctccacc agttcgaaac gcagccaggg aagacgctca atctggcgca ggatttttgg
    11041 ttgctgacgc agggcgatga gcgtagggcc atcaatattg accgatgcca gcaggccgtg
    11101 ctctataaag aagtcggctt tttgcgccag caaatcaatc tgctctttca caacctccat
    11161 ccgatggctg acggtgattt cagtaaaata gcgatccggc ggcaggcgtt gcgaagggtt
    11221 caagggatgc gtgaccaccg ttaatagctc cacggccatt aaccgcccgc atgtttgata
    11281 gatcggctgc caggtgtaag cacgctcaca ctgcaaccaa aaacgccgtt cctgcaagct
    11341 ctcgatgctt gcttcagggt tgcttattcg ctggataacc tgccttatca tcaaagatgt
    11401 cctgtgttta aaagtgatgc tgcccggact cgacaaagat tatcggcgcg taagggcaga
    11461 actttaccgc ctggccgggg taaatttcct cactccacaa cggaatcttt caggaatacg
    11521 ttctggctca aaaaaataat ggaacactgt tttaatatgg ttgaccagca aaccaccaca
    11581 gcgcaaacta acgctaattt tttacagatc aggttcacga ctatgtccaa aaagattgcc
    11641 gtgattggcg aatgcatgat tgagctttcc gagaaaggcg cggacgttaa gcgcggtttc
    11701 ggcggcgata ccctgaacac ttccgtctat atcgcccgtc aggtcgatcc tgcggcatta
    11761 accgttcatt acgtaacggc gctgggaacg gacagtttta gccagcagat gctggacgcc
    11821 tggcacggcg agaacgttga tacttccctg acccaacgga tggaaaaccg tctgccgggc
    11881 ctttactaca ttgaaaccga cagcaccggc gagcgtacgt tctactactg gcggaacgaa
    11941 gccgccgcca aattctggct ggagagtgag cagtctgcgg cgatttgcga agagctggcg
    12001 aatttcgatt atctctacct gagcgggatt agcctggcga tcttaagccc gaccagccgc
    12061 gaaaagctgc tttccctgct gcgcgaatgc cgcgccaacg gcggaaaagt gattttcgac
    12121 aataactatc gtccgcgcct gtgggccagc aaagaagaga cacagcaggt gtaccaacaa
    12181 atgctggaat gcacggatat cgccttcctg acgctggacg acgaagacgc gctgtggggt
    12241 caacagccgg tggaagacgt cattgcgcgc acccataacg cgggcgtgaa agaagtggtg
    12301 gtgaaacgcg gggcggattc ttgcctggtg tccattgctg gcgaagggtt agtggatgtt
    12361 ccggcggtga aactgccgaa agaaaaagtg atcgatacca ccgcagctgg cgactctttc
    12421 agtgccggtt atctggcggt acgtctgaca ggcggcagcg cggaagacgc ggcgaaacgt
    12481 gggcacctga ccgcaagtac cgttattcag tatcgcggcg cgattatccc gcgtgaggcg
    12541 atgccagcgt aaacgcagca tatgcaaaag gctggataaa gtgttacccc tctatccagc
    12601 tcatccataa atacctgatg gttgtgctaa ctcatcacca tcaggcatta ctgtgcaggt
    12661 ggaatatccg tcacttcagg atgtacgtca tccgtggcga cagaggtggt cgcagcggcg
    12721 gtagatgggg ccatgatttg atcccagacc gcctgcaacg ctttcatgtt aaattccggc
    12781 tcgcctttcg gctgcagcag tattaacgcc atatcattcg acaactgttg acgcagatcc
    12841 tgatttaaca tctctaccgt caggctatta aggaaatcct gccgcagttt ctgatattgc
    12901 tccggcgcga tatcgacaac ctgattttgc aacgaacgca tccgctgacc catcagaata
    12961 tcggtatcag ctcgtgcata ggcggcaaac agtttctgca gctccagttt cttttgcgcc
    13021 actaacgcat tgaactcttc ttccggcaga cctttatcgc gaaccttcgc cagttcacgc
    13081 gccaccagat taaggttgct gttcagcttg tcgtttggtg attcgatgtt gatggcacac
    13141 tgcgcacgca gatacagcac acggcagtca aatccaagac cgatgtcttt gctgttactg
    13201 gcacttaacg cttgctgaac atgccagaac agcgcctcgc gggccaggtc cgcacgccag
    13261 tagcgcagca gtgcggctga ttcacgaatc ggctgccacg gcgtatccca catgatagat
    13321 aaccggtcct gacgcaccgc gtcagtcata atactcaccg cttccgcacg cagcggagaa
    13381 agcgtcggca ccggagctgg cgtttcacgt ttgcctttca gttcgccaaa cgttttgttt
    13441 atttggtcga caaccgagcg cgcatccacg tttcccacca ccagtagcgt cattgcatcc
    13501 ggggtgtacc atttctgata gaaatcttta attttttccg cttcaacggg ttgtttcagc
    13561 ggatcggcag gatcgtgacc taacaaggtt gaccctttca gacgatagcg ccaccagccc
    13621 tctttagtat cggcaggcca ggttgccacc atgtcctgac tttgcagcgc gtggttgatg
    13681 gtttctggtg tgatggtcaa tttgccagtg gcatttgcca gataagagag cgcttctttc
    13741 agcaaatcgt tacggttatt gggcaaactc agattaaaca gcgtggtgtc ataagagaca
    13801 attaccggcg gcatcgggcg tttagggtcg atcccctgct gccacaatga acgcgcctgt
    13861 gctgcgtcaa ggccaccgct ttgcgttagc gcaatacgag ggatggcgtg actgtaaccg
    13921 ctctgttgtg tactttcggc gagcgaaccg gtattaacca gcaggcgaat ttcaacacga
    13981 tcgctgggac gctggggggt ggtcagcact tgccactgta aaccgttgga aagcgtcccc
    14041 tgttgccatg ctggatcagg ctggagcgca tctgcctgca catagccagc agtggccatc
    14101 atcagcaaac cgcccgctaa aagtcgaatt tttgtgccct gcatgtgaac ccctgatcaa
    14161 ctatcctggt aataaaaaaa ctgcccgttg tcggtcagtc taaatgacgt taaaaacact
    14221 tcgtgttaga ccgcaagaac atgaaaatgt cacggaagaa gtgaaataaa cctgaactca
    14281 cccaggtggt aagttcaggc acaggggtca attatgcgca aacacccgca ctcggggaag
    14341 ggagtgcggg cataagtgat gagattaaga ggataattcg tgcgttttgc catccggcgc
    14401 acgattattc agcacatcgt ccagtttttt gtggtccagt tctttcaccc acttagcaac
    14461 gacaatggtc gctacgccgt taccgaccag gttagtcagc gcacgagctt ctgacataaa
    14521 gcggtcgata ccgaggatca gcgccagacc cgctaccggc aaatggccca ccgcagagag
    14581 cgtcgccgcc agcacgataa agccactacc cgttacccct gccgcccctt tagaagaaag
    14641 cagcaacacg attaacagcg tgatttggtg gacgatatcc atctgactgt tagtggcctg
    14701 ggcgataaac accgccgcca ttgtcaggta tatcgatgtg ccatcaaggt taaacgagta
    14761 gcctgtcggg atgaccagcc ccaccaccga tttacggcag ccgagtttct ccatcttgtc
    14821 gagcatacgc ggcagcgccg actcggaaga tgaagtcccc agtacaatca gcagttcttc
    14881 acggatgtag cggataaatt tgaagatact gaaaccagtc gctttagcga ttgaacccaa
    14941 taccagcacc acaaacagga tacaggtaat gtagaaacag ataatcagct gccccagttg
    15001 caccagtgtg ccgacgccgt atttaccgat ggtaaacgcc attgccccga acgcaccaat
    15061 aggtgccaga cgcatgatca tattgatgat gccgaagatg acctgcgaga aactttcgat
    15121 gacgttaaaa atcagttggc ctttgctgcc cagacggtgg agcgcaaaac caaacagtac
    15181 ggcaaacagc agcacctgca gaatgttacc gctggcaaat gcgccaatga cgctcgccgg
    15241 gatgacatcc ataatgaagg cgacaatgcc ctggtctttc gcctgatcgg cgtaaaccgc
    15301 taccgctttc gcatcaagcg ttgccggatc gacgttcatt ccggcaccag gctgcacgac
    15361 gttaacgatg ataagaccaa taatcagcgc gatggtactg acaatttcaa agtaaagcag
    15421 tgcgactgcg ccggtacgac cgaccgcctt catgctttcc atgcccgcaa tgcccgttac
    15481 gacggtacaa aagatgacag gagcgatgat catcttaatg agcttaacga agccgtcgcc
    15541 aagcggtttc atttgctcgc ctatttcagg atagaaatgg ccaaggagaa taccaatggc
    15601 tatcgctgtc aggacctgaa agtaaaggct tttaaacaga gaggttttca tagggtgtcc
    15661 tttagtaaaa ccacaggtct tgtaaggtta tggggtacct gcggccttaa aataacaccc
    15721 agacaacatc acagaaatgt acctggatca taattgaaac aaaaaggtta aaaagtttga
    15781 gctggctcgc acaaaccagc actttttaaa gttttgtaat cagtttgggg tagctacttt
    15841 tcttccaggt aactctcttc gaagatttca atagggagtg ggcgagcaaa aaggaagccc
    15901 tgggcaatac caacgcccgc ttttgccagc cagtcgcgtt gtgcttcagt ctccacgcct
    15961 tcggcaatca tttgtaagtt caggctctgc gccagcatga taattgcagc aatcatgctg
    16021 ctatctcccg gcaagccttc aacaaacatt ttgtcgattt tcagtacgtc gattggcaac
    16081 gatttcatat gctgcagctg acgcagccct gcgtagccca tgccgaaatc atccagcgcc
    16141 acccgaactc cggcattgcg cagcggacgg aggattgcca ccgcagcatg agggtcgtca
    16201 atacgtcggc tttctgtcac ttccagaatc agtgttcccg gctgaatgcg atagcgggtt
    16261 aacagttcca gcatatccgc caccatattt gggtgcatca gttgcagcgc agagaggttt
    16321 accgacaagg gcagcataat gccgcgctct tgccaggctg caagcaatcg acaggactct
    16381 tccagcaccc agtgaccgac ggtaaccatc agcccacagc actcaatgcg atcgattaag
    16441 ccatccggca ggtcccaact gccatccggt tgctggatac gcagtaacac ttccgcactg
    16501 accagttttc cgctggtcat ctcgacctgt ggctgtaacc aaatagcaaa ctgatgattt
    16561 tccagtgcat taaggatatc gctctcttcc gtcaaccgct tctgggcggc ttccatctgc
    16621 tgcggatcaa agaactgaat ctgattcttg cctttatggc gagcggtaaa tgccgcagaa
    16681 atagcgcgac tgtaaagctg ttcggcggtg agatcgccgt agaacatcgc cacgccaatg
    16741 ctacagtgcg gacggagttg aatacgttca atcggcaggc gctcgctcat gatagtgagc
    16801 acttgctgac ctaaggtgat tgcgtgccac ggttcctgta caccgttggc aatgacagca
    16861 aagtcataac cgctaatctg cgcgaggatc atacgtggcg acagtaccga tttgagtttt
    16921 tccaccagcg tcagcagcag aatttctcgt tgcgcctctt tcagcacgcc cgcagtatca
    16981 cgcagggttt cacaggtgat gatcatcagc gcggtggttt gtttacgcgc gacaacctgc
    17041 tccagcatct ccatcagcaa ggctttgttc ggcaaatccg acaccgggaa gcgcatcgca
    17101 ttttcgttct gttcttcata atggcgctgc agcaattgct ggttgaggtt gtaactgcgc
    17161 accaacatac cgatttcatc gtcctgatgc agacgcggta atgccagttg gtgaccaaca
    17221 agctcctggg ctgggatggc gttaagttcg cgagcaatat tgcgtaacgg atgcaaaatc
    17281 aggcggttaa tgcaccagct gatggcgacg gtcaggataa gcgacaaaag taagtaaatg
    17341 gtcactaacg ttgagagggt gctcatcacg aacttataca tacggaagga atccgcctgt
    17401 agtaccagat aggcaattgg ctgcgggttt gccggacgtt cgagcgagta aacgcccagc
    17461 gagatttgaa ccggtagctc aaacaggcga gtaaccatta ccggcaccgg gcgctctgga
    17521 ataaaacttt tacgcagcgc ctggaactgg ttaggcagca ctacatcggc acggctgacc
    17581 acgcctgccg gtttaatgct ggcaagaata gcttccgctt cgggaatatc gcctttcaaa
    17641 atagctgaag ataagggttc gcggacagag cgagcaatgc tttccagttg cgtagccgtg
    17701 ttatagcgat tctgctggac cagatggaac agcaaaacgg tgcaaaaaat aaaaacgaac
    17761 accaggacaa cggctgccac cattgccatc tgcttgattg ttaacgagcg acttacgcgc
    17821 aaattgtctc cccccggaaa tcccagattg ccgcccgagt atacccgatc gcagcggcat
    17881 taagagaggc gctatctgaa aacttaccag tcggcgtaag gtatcagcgg ctgcggcggt
    17941 aaatccatgt caccctgcca tccggcggcg gaataacgta cgtagagcag gaaatggctg
    18001 ggtgcgtaat ctttcgcctg ctggatatca attgccgtgc caacaaacca gttggaagta
    18061 acacgtcgct caagtaatgc ccgcgccgtg tagccgaagc cctgactgct gccgccatcg
    18121 ttggattgcc gcgcagcttc ttcctgccag tcggtcggga tcagattcat cagcggataa
    18181 cgcggcatgg ttttggtgcg tgaatgcgac cacgagccag acgcacccag ctcccacgac
    18241 cagttttccg tgcgctcccg ccacatcacc ggtatggcaa acgacaggta ttcctgcgga
    18301 ctgtagtaac cgccctgacc gagtgagtag ccactcagat ctttgtcgta atgccagatc
    18361 atgttgttca ggccgattgt gacgcggcga ttgttctggt taatgacctt atagtaatag
    18421 cccgtcatcc agcgcacgcg ccagttatct tcgacatttt taccggttaa ctggtcgcca
    18481 ctaagcgatg cccagacgcc gtttgcttca cctttatcgt agctcagact tagccccaca
    18541 ccgtcggcac gtacgccacc ccattttttc ccggtattgc tcggggagtc tttttgccca
    18601 ccaaaggcca gcaaagaact ggagatgggc cgacggtggg cgttaacggt gtaacccagc
    18661 ggcccgatat catcgctgta actgatgccg ccgaccacat ccaccacgtt gaagcccatc
    18721 ggcgtggtac cgatatccca gctccagacg tcatttcgcc agccgaccgc cacgctggca
    18781 ccggaatccg actggctgcg gttgccgcta cagtcctgta atgtacaggt gccccagttg
    18841 tcatcccatt tgccatcggc attagtggag aaactgccga cgttcatatt gacgaaatca
    18901 ctgcgaaaga acatccgccc gtcagaatac ggcgcatcca cctgcaacat ggtagtgtgc
    18961 gctttcagat cggagtaacc accggtgccg ctcgaacccc agtaatcgtg ctcaagggtg
    19021 acgttaagat cctgctggcg atagaggtcc gccgcatcgc tgcgcacgcc acgtttcagc
    19081 cagtcatctt tctcgtcgtt acgggtcagt cgggtaaagg tgtcgttatc ctgcggacgc
    19141 gtcgtagtca caccggatgc gaccatggcg tctttgtagg tttccagcgc ctgcgttgga
    19201 tcgcccgcct gcgcttcaaa cttcgcacca tcacgcagca ccatcgcgct ttccatcgac
    19261 ggtggctgag attttgcctg cgggatcaac ttattaaacg tccgctgcgc tgctgcggta
    19321 tcgccaagct gcgcctgtgc cagcgccacg cgccgctgtg tgttcagcga ggcgttatcg
    19381 gtagcgggca gtttcgccag ctggctacgt gccgccgctt tgtcaccggc agcaatatcc
    19441 acttccgtca gaccaagaat ggcgtcggcg ttagctggct cccgcgtcag gacattctga
    19501 tatgcagcgc gggcggcggt gtaatcacgt cgttgttgcg cccagtcagc cagcgtgagg
    19561 tcaatacgcg tggaaggtgg ttgctggcgc agcatcgctt ccgcttctgc ctctttgccg
    19621 ctttctcgca ggcggttagc ggtttccagc acctgatcgc tttgcagtcg attaaccagc
    19681 tcctgaatat tgctgttcca ctgcgcacgc ggcaggctgt tgatatgcgc cagcgccgct
    19741 ctgtcctggt catgaccaga gagatacagc ccgtaagcgt aaacctgctc cgggtcattc
    19801 ggcttctgct gcgccagatt gcgcattaac gtatcggcct ggctgcgttg tccggcctgc
    19861 cagagatcct gcgaaagtcg gtaagtaatc catacactgc cggggtccag cgccagtcgt
    19921 tgccgctgaa gtgctgccgc ctgcgcccat ttgccctggt tttccagtgc ctctgcctgc
    19981 tgtgccagac ggtcgttttg caggctgcgt tcgatatcat caatgctacg ccgctgactg
    20041 gcagagagcg aggcgataaa cgcttcagct ttttctggcg attgctggcg gtaaatattt
    20101 gccagcccgc gcacggcgtt agtgttgccg ctgtccatac gcaaggtctg ctgataataa
    20161 cgttcggcgg cggggtaatc ttttcgcgcc atcgccacat cgcccagccc cagcactgca
    20221 taactgtcgg tgttatcgac attacgcgcc tgctggaaca ggcgttctgc ccggtcagga
    20281 ttattggctt tcagcgcagc atcgccctgc tggatcgcca gccagtagcg gttcactttc
    20341 agcagactat tccatttgtc gttgttgctg ctgtgcgggt ccagtgcgag ggctttttcc
    20401 agattcgcca ctgcattggc gcgatcgcct ttctgtgaat atgcctggcc cagcgccccc
    20461 agagcctcac tgtctttcgg gttcgcccgc actgcctgtt gtagttcggg aatggcttta
    20521 cccgccatac cagagtccac cgccgctaaa ccttgcgcac gagcgcggaa agcaggatcg
    20581 gccagctgtt tttgctgttc tgccagttgc gattgcgcag ccgccacgct atcgccatca
    20641 ctaaagatcg aaagatattt tttcagcgcc gacacactgg catcgctgac gggcatgtct
    20701 ttaatctgcc cgtaccagat tttagaggcc ccttcgcgcc cggcgttcga tttcgccatc
    20761 tgttccagga cggcaaaacc ttcgtcacgg cgatcgctac taaacagcaa tagcgccaga
    20821 ttgttttgca ggcccgtatt gcccggcgta tccgcattaa tgcgttttag ctgattaatc
    20881 gcttcgccac ggcgagccgg aattttcgcc accgtactcc agtactcgac agcaatgtca
    20941 ccttccggcg gcgcaccgtt gaacagtttg ttgtaactcg ccacagcttc ttccgcatga
    21001 ccggtagtcg cctgcaatct tgcctgttgc agtgcctgac gaccatccgg cgtggaaagt
    21061 agcatcgtag tccgcgacga tttatacgca tttgaactcg gcgctaactg cgacagccga
    21121 tcgagctgtt tttgcgcgcc atcaatatcg ccctgacgta acaaagaacg gaaacgggcg
    21181 gcaacgacgt ccgggttatt cggatcaata agttccagcc gatataacga ctgttgcacc
    21241 agatcttcac gatgggtcgc ttcgcctaac cgaacttgct ccagcaactg ttgctgagcg
    21301 gttggtgctg cctcgaccat cggcatgacg gccagaccga gggaaagcgt gaatatgttt
    21361 agtgtgaatt tgcgcattcc tggccccagt caggtaataa ctcacctttt gtcgagaagc
    21421 ggaaacggtg ttgatcccag ccttgtccaa acagggtcag cacatagttg taataggcat
    21481 cgctgccggg aaagttatcg gccacgcgct ggcgctgaac ggcctgcgca tcgcggtttt
    21541 gtaaaaaagg cagcatggcg gcagaaaaac cgaccggtcc tttaccctgc gctttccccg
    21601 tagccacatc cactttttcc ggcggataac cgtttttctc agtgaatgtc gccatcggtt
    21661 taaaccggtt gagcatccgc gctttttgcg gatcgctgtc aggcatcatg cctacccaca
    21721 tgtaaacgcg gatagcgtcg tagctgctga tcaatgtttt ttcggctttt agctgccagc
    21781 ctttgtcttt ctcatagcgc acccagtctg gcgaaaagcc tttcggggcg gtttccagca
    21841 ataaacgttg attggtttcg cgcagcgtag tccacggcgc gccaaagcgg gtgaaatact
    21901 gcgccagcgt cggcggcagg tagctggggt taaaacgcca gctgttatcc tcagcaaaac
    21961 ccactttgcc cggtaacaac atggaaccca gcccaggcac cgtcaccact tcctcccgcg
    22021 cgatacgttt tagcaacgcg ctgccgatgt cggtataacg ctgctctttc cacaaacgcc
    22081 ccgcctccag caatgaccag gccatccaga catcaccatc ggaggccgaa ttgctgtcca
    22141 gcacttccca cttactgttc tctttcttgc cccacagcca ggcgggcaaa ccttctttta
    22201 aagaaccctg agcgagattg ttctgcgtcc agtcgagaat attatcgaaa gctgcacggt
    22261 cgttagccgc cagggcaaag aacataccgt aactttgccc ttcggaggtg gtgattttgc
    22321 gcgcgtcgct ggggtcgatg acgcgccctt cctgactgat gtaatccttt ttaaactgct
    22381 cccaggcagg ccaggtacag gctgcctgaa cactaaaggc agccagcagc agcatcgtca
    22441 cgattccact acgcaacaca ttcatcttca attactcgtt atccgggtta agacgacgac
    22501 gactaataat tcgcagcaga cgccacagta cccatgccag caatatcaca ctgatagccg
    22561 ccagcaccgc cagcagaatc ggatggtttg ccagcgcata ccacacgcgc tcgaaccacg
    22621 gcagatgacc tacgtaataa acgtcgccaa cacgtaggct gttgataccg gactcgcgga
    22681 tcaccgcgac cgaaccgaac atggtggcgc gtttgccgct atcgttcacc gcatcgttaa
    22741 gcatttcata accgcgtggg ctatctgcca acagcgcaat cacgctgcgc tggtcgttat
    22801 acggcgactg gaagccaatc accgccgcca tcgcaccgga agaggtcagc gttgaccggg
    22861 tttctgccgc gcgatcgctc tcgtccggca caatgccggg gaacggggtc tggcgcatcg
    22921 gtgttttcac ccagctttcg gtcgcctgca ccaataggtc gatctgctta tcgtctttca
    22981 gtttgtccgg gataccaccg atgatcatga tgtcggcatc tttgccctga atggtgctgc
    23041 catcatcggt caccgtcaga ttaatcgccg ggaagcccgt ctgtgcgccg ataaaaccaa
    23101 cagtattcag caacgtttcc atctgtgctt cgttaggcgc tttcggcatc acggtgatgg
    23161 tttgcgacag atccgccatc cggctgaatg ggaagcccgc gttagcaaag gcgcgtagat
    23221 ccggcatcgg gatgaagtgg taatacttcg agaagtcgat ggtggagtcg tcaccaatca
    23281 ccacatgatt ctgcaccggc tggaaggtaa tacagttatc caccgaaccg cccggcatcg
    23341 ggttcatata ctcaaagtcg aagcgcagct ggttggtcgc gcccagtttc agcgccggaa
    23401 tagagacatc tgttttgcca tccagcaaac cttgtaatac cggaatccgc agcagcaggc
    23461 ggttcgcctc ctgtttgctg ctcaggttga aggattgcag gaactggtta ttcaggctga
    23521 tatccatccg cgaactgtct ttcaccggcg gcatggtgta gcggtaatta atatccatat
    23581 caatgccggt actgcgcatc aggtagagat ccggcggcag gtttagcgaa acgttaatcg
    23641 ctgctggctc aagaccgctg gattgtaact gttcttcata ggttttcagt tcgccaaatg
    23701 tgaccggacg atcggtacgt acccagttcg gcgcatcgta cggcttacgc ggtagcagcg
    23761 gtttcacttc attcactacc acgctttcac cacggaacag aatgttaccc tgagcgatac
    23821 ctttagctgc ctgcaacagg tctttgtcgt cacgaccaaa caccaccagc agtttgacgt
    23881 aaggattctg cggatggtta atcatctcaa tcaccggggc ttttaccgcc ggatgatcgc
    23941 gcaggaagtc cggccgtttg tcgttggttg caaagacaat ggcattacga tccggcagtt
    24001 ggttatagag caccgggaag ttctgcccac gccagccaga acgcgaacca aaccacgagg
    24061 cgacaatggc agaggcttgt tgcagcccaa catccggcgc acccgcaaag accatcggca
    24121 aggtgttagt acggttatcg cgcgggtcaa agaatggcac cgggaagtgt gacaggtcat
    24181 tcttcacatt cagggtctga taggtcagat ccagtccact gctccgccca acatccagcc
    24241 aaagcgtggt gctggccggg ttttcgcaca cgtcctgata atggccgaca aactccagcc
    24301 gtacacggtt gaagtcggta ataaacagtg ggttaatggg catttgcgcc agcgtttttt
    24361 tacccaactg ttctttggtc actggcagca cgcccatcag ttcatcattg agataaacct
    24421 ttaactgcga ctggacaggc agtaacgatg gcgatggggt gtattcgagg ttgagcatcg
    24481 ccttcgtcac cacttcatcg ctgcgcatac caaactcaat gctgccgttc gggttaatgc
    24541 cacgtagcac catgctgccc ggcggcggtg caatttgcgc aaaggtcagc ttcacatcac
    24601 gcgaaggacc gttctgcgcc acgactggcg catcagcgcc ctgcacgccc ggcatcactt
    24661 gccccacctg cggattttgt tccgtctggg cggctacagc tggctcagca ttgatcagtg
    24721 gttgcgttgc tggcgtcgcc tgcgtcatga aagaggggaa cgcactcatc cccatagcca
    24781 ctgcacaaat ccagaatagt tttcttttca tcgcgttatc atcattgttg agccaaagcc
    24841 tgatccgatg gttgtgccgt ttcgctccgc tccgggcggc gcggaataaa cgatacaacc
    24901 caggaaacca gagaagtcag cacacggaat atgcccttca ccgaagaagg cgcaaactcc
    24961 gccagatggc ggtagccacg gaagccgagc ttcagaatat ccagcagact ttccagcggc
    25021 ttatcttccg ggtagctgtc ctgccagagc gcccatgtat ccgcacgggc aaacgtacac
    25081 tgcacaaaat cgatatgttg ctgggtggtg agcggcatta atttcagccc aacttcatta
    25141 cccatcacgc gcgccacctg ggtcgggaag acgtattcct gctgaccgcg tttaagcaac
    25201 agattcactt tctgcccttc cagaatctgc gcctgaccgt tgatcttgat ccccaaacca
    25261 ccgtcggaga aatcctgaac ggtacacgag aagaggtgac catcttcgcg ggcaattgcc
    25321 gcgggcatcg tcatctccac gcggtgcgat cggcgtacct gtttgctttc taccgatacc
    25381 gcaactgcgc cgccaagaac aatcaggttg tagaacaccc acaccatact gacgaccacg
    25441 gtgagcatct cggttggcgg gccatagaag tagcgccaga tgcctaccgc aacgcccacc
    25501 aggttgagca ggacaaggaa gatgtagggc cgcgagatca cccagtcgac gtactcttct
    25561 tccaccagtc cacctttggc ggtgacgtta aatttgcctt tgtgcgggtt aatcagcgcc
    25621 accagcgtcg gtggtgcgat ataccacgcc agcaccgttt cgtagatttc actccagaaa
    25681 gagtggcgat atttgccctg gatcttggag ttggtcaggc tggcatggat catatgcggc
    25741 agcacgaata gggcgatcat caacgctggc gcatagatga tgtaggcatg aagcagcagg
    25801 aacgccagcg gcgcagtcag gaagatcagc cgtggaatgc ccgacaagaa gtggaacatg
    25861 gcgttgacgt aacatagccg ctgagcaaac ttcagccctt taccggtgag cgggttatcg
    25921 agacggaaga tttgtaccat cccgcgcgcc cagcgaatac gctgaccgat atgcgccgac
    25981 agactttcgg tcgccagccc cgccgcctgc ggaatacgca tatacgcgga ggtatagcca
    26041 cgacggtgca accgcagaga agtatgcgca tcttcagtca cggtttcgac agcaatgccg
    26101 ccaatttcat ccagcggctt acgacgaatc accgcacagg aaccgcagaa gaaagtggcg
    26161 tcccacatat cgttgccatc ctgcaccaga ccatagaaca gcgtgccttc gttcggcgtt
    26221 ttacggaaac gccccaggtt gcgttcaaac gggtccggtg agaagaagtg gtgcggcgtc
    26281 tgcatcatcg ccagctgttt ttctttcagg aaccagccca tggtcatttg caagaacgat
    26341 cgcgttggta cgtggtcgca gtcgaaaatc gacacgaact cgcctttggc atatttcagc
    26401 gcattgttga tgttgcctgc tttcgcatgt tcatgagtgg tgcgggcgat atatttcacc
    26461 cccacgtttt gcgcaaactg gcgaaactct tccctaccgc cgtcatcgag gatccagatg
    26521 ttcagcttgt ctttcggcca gtcgataccc agcgaggcgt aaatagtatt tttcaccacg
    26581 ttgagatctt cgttgtaagt cgggacaaag atatccaccg acggccacag cgacatatct
    26641 ttcggcaatg gcaccggctg acgattcagc ggccatacta cctggaagta gccgagcacc
    26701 agcacaatcc acgcgtacgt ttcagcgaag agcagaataa gcccgcacac caggctgacc
    26761 ggatcgtccc agttcagcgt agaggtgtaa cgccaccaga tataacggca agaaacggtc
    26821 agcgacagca caatcaacat tagcgccgag aagcgccccg gcatccgccg tacgatcagc
    26881 gctacacccc acagcagcat caggaagata aactgcgcca gcgggttaaa cggttgagta
    26941 acgcagatta acgccagaat cagcgagaag gtgacgatga taccgaggat caaccgccgc
    27001 gccccggcac tcaaatgacc gagctctttt ttctcatcaa gatgctgtgt tttatggcta
    27061 acgcgctcag gcagctcgtt catccattga tggtaacgtc cacgaatatt ttgcagacct
    27121 gaaaatgccc gcctgcgcgg tttcggcgtt tctttgcgcg atgcaccgat caataaccag
    27181 catgtttgaa tgagataacg gaccgggtcc agcggacgcg gacgcgaggc gttgatatgc
    27241 ggatacaggt ttttatgttc tgcgcgaata cgctgccagc gcgggtgctc cagcggaata
    27301 aaaatccagg ccaggatcat ccagaaacag ccgagcgtcg cgctgaaagc cgacgcaccg
    27361 tgacgacgat aatcgcgata acgcccgata agccgcgcgt tgaccggcgg gataagcaac
    27421 caccgggtca ggatactcat gatgcactcc cgactggcgt tttcagcccg gagtagttca
    27481 acaggcacca gttcgccagc gtcagtatct cttcagccgc cagcgcatca ctgcggtatt
    27541 caccgactgg ttgcttagcc gccaggcatt cagccatcgc ttcatcacga tgaatgagca
    27601 tcggcagcaa tcggcgctgg ctttgcaacc aaagctggta aatatcgtcc tgaacctgac
    27661 tgccaatacg gaagtcatta atcaaaatat gtgcgccatc cggcagcgct tgctgatgca
    27721 gtcggatatg gcagttggca tccacattga cgattgccag cgagtgatcg cacaaactca
    27781 gcagctggtg ggttatctgc gaggcatcac gcggtaagtc gattaaaatc cactggtaac
    27841 gcccgctggc ttttagttgc tgtaagccgg agcaaatatc gctcagccgg gtttgccagt
    27901 gctgtggatt ttcttgttct tcaatggata actgaccaaa aggcagcaaa tcgagctgcg
    27961 aggtgtagcg caacccagcg tcacgccagt cctggccatc cagcatcgct ctggcccagc
    28021 cctgacggtg ggtaaaatca acattaaatg acaggcgcaa caagttgtcc gggcaggcat
    28081 cgaccaccag aacattttct cccaacattt gtaatgacca ggctaatgcg gcggtgatgg
    28141 ttgttgtccc cacgcctccc cgcaccccct gcaatcccag tacggccatt caccaatccc
    28201 ctacttttgt tgcgcaaact ctgccagcaa cggccagcgt tttaatgccg cggccaactg
    28261 ttcgcgttgg gaaatatcgg cataatcaat atcaggcagt gaaaatgcct gctttaacgc
    28321 cacaatatca ttctggaaga tatagcctat cgcgggatca ggcagagtat ctggttcatt
    28381 gttattcatt ttgcttgatc cctatgaaat aactgtaccg ttcatgagac aattctaacc
    28441 gtgagcattt cgccggttta ttgtttacgg cgagacggga atttgctcac tttttgactt
    28501 tccgttacgc aacagaaaaa gttaaaaacg aaaagactaa aatcacacgt cttgtgacct
    28561 tctaaattga ttcacatgct aaat
//
LOCUS       DAZFZC010000067        28349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFZC010000067 DAZFZC010000000
VERSION     DAZFZC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.067"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            337..951
                     /gene="evgA"
                     /locus_tag="ACHKXP_003193"
     CDS             337..951
                     /gene="evgA"
                     /locus_tag="ACHKXP_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO7648145.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            956..4549
                     /gene="evgS"
                     /locus_tag="ACHKXP_003194"
     CDS             956..4549
                     /gene="evgS"
                     /locus_tag="ACHKXP_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO7648146.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKITLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4605..5750)
                     /gene="yfdE"
                     /locus_tag="ACHKXP_003195"
     CDS             complement(4605..5750)
                     /gene="yfdE"
                     /locus_tag="ACHKXP_003195"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO7648147.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5824..6768)
                     /gene="yfdV"
                     /locus_tag="ACHKXP_003196"
     CDS             complement(5824..6768)
                     /gene="yfdV"
                     /locus_tag="ACHKXP_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO7648148.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLFVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6838..8532)
                     /gene="oxc"
                     /locus_tag="ACHKXP_003197"
     CDS             complement(6838..8532)
                     /gene="oxc"
                     /locus_tag="ACHKXP_003197"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO7648149.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8586..9836)
                     /gene="frc"
                     /locus_tag="ACHKXP_003198"
     CDS             complement(8586..9836)
                     /gene="frc"
                     /locus_tag="ACHKXP_003198"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO7648150.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWNGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10349..10984)
                     /gene="yfdX"
                     /locus_tag="ACHKXP_003199"
     CDS             complement(10349..10984)
                     /gene="yfdX"
                     /locus_tag="ACHKXP_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO7648151.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AAERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKK
                     TNLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVM
                     ENQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11280..11555
                     /gene="ypdI"
                     /locus_tag="ACHKXP_003200"
     CDS             11280..11555
                     /gene="ypdI"
                     /locus_tag="ACHKXP_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO7648152.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGGSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEMPPPFPTKANYLIHPVR"
     gene            complement(11632..11874)
                     /gene="yfdY"
                     /locus_tag="ACHKXP_003201"
     CDS             complement(11632..11874)
                     /gene="yfdY"
                     /locus_tag="ACHKXP_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO7648153.1"
                     /translation="MINLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            12227..13147
                     /gene="lpxP"
                     /locus_tag="ACHKXP_003202"
     CDS             12227..13147
                     /gene="lpxP"
                     /locus_tag="ACHKXP_003202"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO7648154.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13503..13574
                     /gene="ypdK"
                     /locus_tag="ACHKXP_003203"
     CDS             13503..13574
                     /gene="ypdK"
                     /locus_tag="ACHKXP_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO7648155.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13639..14877)
                     /gene="alaC"
                     /locus_tag="ACHKXP_003204"
     CDS             complement(13639..14877)
                     /gene="alaC"
                     /locus_tag="ACHKXP_003204"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO7648156.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            15254..16951
                     /gene="ypdA"
                     /locus_tag="ACHKXP_003205"
     CDS             15254..16951
                     /gene="ypdA"
                     /locus_tag="ACHKXP_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO7648157.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            16966..17700
                     /gene="ypdB"
                     /locus_tag="ACHKXP_003206"
     CDS             16966..17700
                     /gene="ypdB"
                     /locus_tag="ACHKXP_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO7648158.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            17713..18570
                     /gene="ypdC"
                     /locus_tag="ACHKXP_003207"
     CDS             17713..18570
                     /gene="ypdC"
                     /locus_tag="ACHKXP_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7648159.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKQVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRSVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18573..21068)
                     /gene="ptsP"
                     /locus_tag="ACHKXP_003208"
     CDS             complement(18573..21068)
                     /gene="ptsP"
                     /locus_tag="ACHKXP_003208"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7648160.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(21093..22130)
                     /gene="ypdE"
                     /locus_tag="ACHKXP_003209"
     CDS             complement(21093..22130)
                     /gene="ypdE"
                     /locus_tag="ACHKXP_003209"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7648161.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWVETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(22130..23215)
                     /gene="ypdF"
                     /locus_tag="ACHKXP_003210"
     CDS             complement(22130..23215)
                     /gene="ypdF"
                     /locus_tag="ACHKXP_003210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7648162.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVVSGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(23230..24477)
                     /gene="fryC"
                     /locus_tag="ACHKXP_003211"
     CDS             complement(23230..24477)
                     /gene="fryC"
                     /locus_tag="ACHKXP_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO7648163.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(24499..24825)
                     /gene="fryB"
                     /locus_tag="ACHKXP_003212"
     CDS             complement(24499..24825)
                     /gene="fryB"
                     /locus_tag="ACHKXP_003212"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO7648164.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(25044..26009)
                     /gene="glk"
                     /locus_tag="ACHKXP_003213"
     CDS             complement(25044..26009)
                     /gene="glk"
                     /locus_tag="ACHKXP_003213"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO7648165.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            26213..27469
                     /gene="yfeO"
                     /locus_tag="ACHKXP_003214"
     CDS             26213..27469
                     /gene="yfeO"
                     /locus_tag="ACHKXP_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO7648166.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            27584..27910
                     /gene="ypeC"
                     /locus_tag="ACHKXP_003215"
     CDS             27584..27910
                     /gene="ypeC"
                     /locus_tag="ACHKXP_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO7648167.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(28051..>28349)
                     /locus_tag="ACHKXP_003216"
     CDS             complement(28051..>28349)
                     /locus_tag="ACHKXP_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFO7648168.1"
                     /translation="QGFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSF
                     GIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
BASE COUNT         7436 a         7100 c         6603 g         7210 t
ORIGIN      
        1 tgtgttgcac attatcccag gaacgttgga ggcctaattt aaatgaggtg caaaaaaaac
       61 aacagaggac taaaccgtgg cttttgcaat acaattctta cgcctgtagg attagtaaga
      121 agacttatag tgccaacttg aaactataaa tcatcggtac aatccctgat tttattgttg
      181 acatttcatt tatgccgact atttatatgg tatacttgtc gaattatctt aaaggaagct
      241 cagattttct tatttttatt gagaaaatga gatgacgcct tatgtctgta ttactacagg
      301 gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat tattgatgac
      361 catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat tgaaatctta
      421 gcagagttga ctgaaggcgg aagtgccgtt cagcgggtgg aaacacttaa gcctgatatc
      481 gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga aacgctgagg
      541 aagcgccaat atagcggaat tattattatc gtctccgcta aaaatgacca tttttacggg
      601 aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg catgaacaat
      661 atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt ctctctcaac
      721 cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc gaaacaagaa
      781 attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc tgaaaaaatg
      841 ttcatcagca acaaaactgt cagcacttat aaaagtcgcc tgatggaaaa attagaatgt
      901 aaatcactga tggatcttta cacattcgca caacgtaaca aaatcggcta accacatgaa
      961 gtttttaccc tatatttttc ttctctgttg tggtctttgg tcgaccataa gtttcgcaga
     1021 cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac ttgatccact
     1081 acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg tgattgcagt
     1141 acataagtcc caaacggcta cgttgttgca taccgattcg cagcaacggg ttcgtggtat
     1201 taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaaataa cactccggga
     1261 atacgcagat catcaaaaag caatggacgc gcttgcagaa ggtgaagtcg atatagtgtt
     1321 atcacattta gttacttcgc cgcctcttaa taatgacatt gctgcaacca aaccattgat
     1381 aattaccttt ccggcgctgg taaccaccct tcacgactca atgcgaccgc ttacctcacc
     1441 aaaaccagta aatattgctc gggtagcaaa ttacccccca gacgaggtaa ttcatcaatc
     1501 atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag catccgtctc
     1561 agctgggcac aatgattact ttattggtag taacatcatt accagcagta tgatttcccg
     1621 ctatttcact cactccttaa atgtagtgaa atattataac tcgccgcgtc aatataattt
     1681 tttcttgacc agaaaagaat ctgtcattct taatgaagta ctcaatagat ttgttgatgc
     1741 tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag gaaacctggc
     1801 ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta agcagcatcc
     1861 caatttaaag gtgctggaaa atccttactc gcccccctat tctatgacgg atgaaaatgg
     1921 ctcggttcgg ggcgttatgg gggacattct taatattatt accttgcaaa caggtttaaa
     1981 tttttctccg atcaccgttt cacacaatat ccatgctgga acacagctta gccccggagg
     2041 atgggatata atacctggcg ctatttatag tgaagatcga gaaaataatg ttttatttgc
     2101 tgaagccttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg acagtgaaca
     2161 aacattaaaa aaaggaatga aagttgccat tccatattat tatgagctgc attcgcaatt
     2221 aaaagagatg tatccggagg ttgaatggat acaggtcgat aatgccagcg ctgcatttca
     2281 caaggttaag gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt cgcgttacat
     2341 gatcgatcat tactatccta atgaacttta tcattttctt attcctggcg ttccgaatgc
     2401 atcgctttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta ttaataaagc
     2461 actgaatgca attcccccaa gcgaagttct gcgcctgacg gaaaaatgga ttaaaatgcc
     2521 caatgtgacc attgacacat gggacctata tagcgagcaa ttttatattg ttacgacatt
     2581 atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac gctcagttcg
     2641 tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata tcattccgaa aagcactctc
     2701 ggattcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca ttagtcataa
     2761 tagtgctttt gaacattatt tcactgcgga ttactacaaa aatgcaatgt taccattaga
     2821 aaacagtgac tcacccttta aagatgtttt ttctaatgcg catgaagtca cagcagaaac
     2881 gaaagaaaat cgaacaatat acacacaggt atttgaaatt gataatggca tcgagaaaag
     2941 atgcattaat cactggcata cattatgcaa tcttcctgca agtgacaatg cagtatatat
     3001 ttgtggttgg caagatatta ctgaaacgcg tgatctaatt aatgcactcg aggtagaaaa
     3061 aaataaagcg ataaaggcta ccgtagcaaa aagtcagttt ctggcaacga tgagtcacga
     3121 aataagaaca ccaataagct ctattatggg cttcctggaa cttctgtcgg gttctggtct
     3181 tagcaaggag caacgggtgg aggcgatttc acttgcctac gccaccggac aatcactcct
     3241 cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact atcaacttca
     3301 accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt tcggtgcgat
     3361 tgctgcaagc aaatcgatcg cattaagttg cagcagtacg tttcctgaac attacctggt
     3421 taagatcgac cctcaggcgt ttaagcaggt cttatcaaat ttactgagta atgctctcaa
     3481 atttaccacc gagggggcag taaaaattac gacctccctg ggtcacattg atgacaacca
     3541 cgctgttatc aaaatgacga ttatggattc tggaagtgga ttatcgcagg aagaacaaca
     3601 acaactgttt aaacgctaca gccaaacaag tgcaggtcgt cagcaaacag gttctggttt
     3661 aggcttaatg atctgcaaag aattaattaa aaatatgcag ggcgatttgt cattagaaag
     3721 tcatccaggc ataggaacaa catttacgat cacaatcccg gtagaaatta gccagcaagt
     3781 ggcgactgtc gaggcaaaag cagaacaacc catcacacta cctgaaaagt tgagcatatt
     3841 aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa atctattagg
     3901 atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca gtatgcaaca
     3961 ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg agttgactcg
     4021 caaactccgt gagcaaaatt cttccttacc catctggggg cttacagcca acgcacaggc
     4081 taacgaacgt gaaaaagggt taagttgcgg catgaactta tgtttgttca aaccgttgac
     4141 cctggatgta ctgaaaacac atttaagtca gttacaccaa gttgcgcata ttgcacctca
     4201 gtatcgccac cttgatatcg aagccctgaa aaataatacg gcgaacgatc tacaactgat
     4261 gcaggagatt ctcatgactt tccagcatga aacgcataaa gatctacccg ctgcgtttca
     4321 agcactagaa gctggcgata acagaacttt ccatcagtgt attcatcgca tccacggtgc
     4381 ggctaacatc ctgaatttgc aaaagttgat taatattagc catcagttag aaataacacc
     4441 tgtttcagat gacagtaagc ctgaaattct tcagttgctg aactctgtaa aagaacacat
     4501 tgcagagctg gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa tagcggctcc
     4561 cacaatgttc aaatgtggga gctatttacc agcacatctt acatttatga tgagaactcc
     4621 tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac atgcgggtcc
     4681 gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcccccggc ttcaatcaac
     4741 atatttctcg cctgagtttg tggcaattta atggcctcag ccacacttaa taacggcgcg
     4801 acgggtacac caacttcatg tattctggct aaccaaactt cagctgcctg cgtttttaac
     4861 gtccgctcaa tatattgttt aagaatagcc tggttttgta cgcgtaaaat attgctgcta
     4921 aatcggggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc agaaaaaagc
     4981 ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tattgaaaac atcaaaaggt
     5041 gccatgtagg gatggcgatt tcccagacgt tgtggtgact tccctgtcgc gatatatgcc
     5101 atcagaccat gctccagaaa actcagcgtg gcatcaaaca tcgctatatc gacatgcgcc
     5161 cctctctggc tcttttcacg gccataaagt gcactcacta ttccgctgaa taaatagaca
     5221 ccgccgcata gatccgcaag agatgtacca acgcgcactg gcggagcatc aggatatcct
     5281 gtttccatca ttatcccgct cattgcctga atgatggtat cgtaggcagg agcatctttt
     5341 agcggaccgg tatgtccgaa acctgacgat gaagcatata tgaggcgcgg gttgatttct
     5401 tgaagcgttt cccatgaaaa ccccagtttt tccattgtac ctgggcgaaa attctcagct
     5461 aatacatcag cttgtttgag catatttata aatatacttt tatcgtgatc attctttaaa
     5521 tcaagaacca cactctcttt gccatgatta ataaaactgt aatagagtga ctgtccatcc
     5581 acatagggac caaatgtgcg ggtatcatca ccatgacccg gcggctcaac tttaattacc
     5641 cttgcgccca tattacaaag aagttgagtt ccgaaaggtc cattaaggac atgtgtcata
     5701 tcgataacta ataagccttc aaacgggcct ttgctttcat tatttgtcat ttgcttatgc
     5761 cttgaaaaaa gccgaaggtg aaaagacact tcggcattta tgtaaaatca ataacatgat
     5821 taattatgaa accagtcgac tgacataaat ccacaaggga gccgtgacga caaagcccag
     5881 tacgctcaca gccaatgacg ctgtaccagt acgggtgtag acattaaacc ggctggcaat
     5941 aatgattccg gagaatgccg gcggtaatgc gcctgccagt accatcatct gcagatgttc
     6001 gctgttcaaa tgacatgcca taccaacgaa aagcagtgcc agtggcatca gaatcagctt
     6061 caggaaggtg ttataagcaa tttcggcact gaactcgaat ttatgtgcag ccagagtcaa
     6121 ccccgcagcg aataccgcta cccctgagtt agccttcgca atcagattaa aggttgggtc
     6181 ccatgctgcc ggaattttta ccccaaccaa caccaggatc gttgccagaa caggtgccca
     6241 tactactggc tcctttgccg cagaaattaa tgcgctcaga ttactattct tcttaccatc
     6301 cgctcctgaa gaaggattca gcaaatacag accaatagga atagtaattg cgttaacaat
     6361 aatagaaata attgctacca ctaaacctgt tgatacggaa tcaccataaa taggatcgag
     6421 aactgcaaac cccaggaatc caatggtagg tgaacctgca attaatgcac atacagctgc
     6481 ttctgcatgg gtacgtttaa aaaatttgta gcaaccgaac caggagaaaa agaaacatcc
     6541 gacaataacc acaagtgata caagggtcag acgagtgtcc gcaaaaatca tttccctgtt
     6601 tgcccgagta atagatacaa atagagccgc aggaagcgcg tagtttaata ccagtttatt
     6661 aaatgcccga gcttgatctt ctgaaaatgt ttctcgtctg ccgctaaaat aacccaacag
     6721 catgattacg ataataggca ataaatcgcc aataaaaaat gttagcataa tagtatttcc
     6781 gataagggtc ccctcaccat cgcataatga gttggcaaga ggagacgatg tcagtattta
     6841 attaccagcg acttgttttg ggttaagttt ggtaatatgg ccactttcag ttcctgctgc
     6901 agggtcgatg accacattaa taatggtcgg tttgcgcgac tgaataccgg tggttaaagc
     6961 atgacgaagt tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg catccattaa
     7021 tttgtcatac cttgcatggt gcaacagatc cgttggtgat ggtgcaccag cgccactgag
     7081 atcaacaccg tctcctctgt agatgccgcc attattaaaa ataacgatcg tcaccggcag
     7141 gttatatcga caaatcgttt caatttccat cccactgaaa ccaaaagcac tatcaccttc
     7201 aatggcgaca accggagaac cagaagtcac gctagcaccg atggcatagc ccataccgat
     7261 gcccatgaca ccccaggtac cacaatccag acgacgacgt ggtttataca tatcaataat
     7321 atttcgtgca ttatccaggg tatttgcacc ttcattaact aaataaatat cctggttctc
     7381 gcgcaatacg tcgcgcacag cacttaatgc attaaagtaa tttaatggtt gagtatctgt
     7441 acttaatttt tcatgcattt tttgtgcatt ttgctgcttg tggatattta aaatatcgcg
     7501 ccataccagt ggagtcgtaa atgtgttttg tttcagttct gccagcatac cttgcatact
     7561 ggatgcaata tcaccaacga ctggcacagc aatggggcgg ttgctgtcaa tttcctgcgg
     7621 ttcaatatcc agttgaataa actgtgtatc tgccgcccat ccttttttac cgtgtgccaa
     7681 taaccaattc agtcgtgcac caacaagcat gacaacgtca gcatttgcca gggcaaacga
     7741 acgcgcagct gccgcagaaa gtggatgcgt atcttcaagg atccctttcg ccatagacat
     7801 tggcaggaat ggaatctgag cactttcaat aaattcacga agctgttcat cagcttgtga
     7861 atacgccgcg cctttgccaa ggataattaa tggccgttca gcttttgcta aaagcgaaat
     7921 tgcgctagtg actgacttcg ggcatggcaa taatgctggc gacggatttt caactttaac
     7981 aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc aggacatttg ctggcaaatc
     8041 aagataaact ccgccagggc gacccgatac agagacccgg atagcgcgtg ccaatgcaat
     8101 gccaagatcc tgcggctgat taacgcgaaa tgctgctttg gcatacggtt ttgccgcatt
     8161 catttggtcc agctcttcat aatcaccttg ctgtaggtcg acgatcgcgc ggtcgctgga
     8221 gccgctaatc atgatcatcg gaaaaccatt taccgttgcg ttggccaatg cggtcaaacc
     8281 attgaggaat cctggcgcag aaactgtcag gcagatcccc ggtttttggg taagaaaacc
     8341 gcttgccgca gcggcatagc ctgccgactg ctcatgacga aaaccaatat aacgaatgcc
     8401 ttccgcctgg gcatggcgtg ccatatccgt cacaggaata cctacaacac cataaatagt
     8461 gtcaatatta ttctgtttta atgcttcaac gatgatatgc ataccatctg tcatttgaag
     8521 ttgatctgac atgattaata acctcgaaat ttttataaat tgggagccag aagcccccgt
     8581 taatatcaga tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct ataacccagc
     8641 tcctgcaata cagcagcggt atgttcacct aatagcggcg cagctttaat atccggcgta
     8701 aaggcagaga atttcattgg acaaccaacg gtcagatatt ttccacgcaa cggttgttcc
     8761 acttcaacaa cactgccact ttggcgcaaa gagggatcaa gtgaaatttc tttcatactt
     8821 aaaaccggtg cacaaggaat atcaaactga gtcaaatagg ccaccgcttc atgtttatca
     8881 atagtgacag tgtatttttc gatttcagca aaaatatcga aaatatgtgg ctgtcgtgca
     8941 tgggctgtac tgtatgccgg atcggtaatc cattctggtt tgccgatggc tttacaggtg
     9001 ttttcccagt tttgctcctg aatagtgaaa taaatatagg cgttaggatc ggtttcccag
     9061 cctttacatt tcaggatcca gccaggctga ccgccaccac ctgcattacc accgcgggga
     9121 actgcatcac caaatgtacc attcggatac tgcgggtatt cttccagata acccaattta
     9181 tcgagacgct gctggtcacg taatttcacg cggcaaaggt tcaatacggc atcctgcatt
     9241 gacatggtga ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc agcaagtaaa
     9301 ccgatcagca aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct taccagcggc
     9361 ggaccattcc aaaaacccgt agtggatgcc gcgccacccg ctgcctgagc aacgttttca
     9421 taggctttta cattcacata aggcgaacac tcatcaaacc ctttgatcga accaaaaatc
     9481 agacgtggat tgatttcttg aatatgctcc caggtgaagc ccatgtgatc aatggcccct
     9541 ggatgaaagt tctcgactaa gatatcagct tcgcggatca gcttttccat tacctctttg
     9601 ccttccgctg ttttggtatt taactcaata gaacgtttgt tactgttaag catggtgaag
     9661 taaagcgcat cgatatcagg aatatctcgc agctggtgac gcgttacgtc accaacgccg
     9721 ggacgttcaa ttttaataac gtcagcgcca aaccaggcca gcatttgagt acaagatggg
     9781 ccagattgca caccggtgaa atcgagaact ttaattcctt gaagtggagt tgacatagca
     9841 atacctatta gttaattaaa tatgtgttaa tttagcgttt atttaaggcg taatatcttc
     9901 tataaataac ccgcaaaata tcgtcgtagc tgcactcgat atttttaaaa tcctgtatca
     9961 agtgctgaaa atcatttcat cgccacttcc aattcctttc tgcatggtaa gtagtgagga
    10021 cgaaaagaag aatatgataa atattgattc gtggtaatca gagagagtcg cgacatttgt
    10081 gatgttattc ttacaactgt aagaagcgcc ggtgatttac aagcaagggt atgattacgc
    10141 tttttaaagc gtaccataaa acataacata ataaatgatt tgtgatgaca cgggattatt
    10201 gcggtttact ttgaggaata taaaatgcga gtttatagca ccgcgagcgc caaataattt
    10261 tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt cggctggagg gagttaaatc
    10321 ctttacctca atatactgcc tgtcagcatt agttcacaaa cagtgcttcg ctatcgacga
    10381 tgattccatc ttccgcacct ttaagcgcca ggtttgcctc gtagtattgc tttttatcaa
    10441 gcagtttctg cgcatcagca agtgcattac gagtctgttt aagtggcatc agatattggt
    10501 tttccattac gccaacaccg gccaggcgaa gctcttccat tgcgcccttt ttatcccctt
    10561 tcgccatttt ttcattggca atttttatcg cggcttcttt ttcgggagtt gctacataac
    10621 tctcagaaat tccgacagat gcattaatga caatatattg gtcatcattg agattggtct
    10681 ttttacctgg cttagcgaac tttgcccatt cggtactgtc atctgacagc aaagcggaag
    10741 cttcattggt cagttctttt gccttttctg gatcgccatg aaacagagcc aggcgcgcta
    10801 cctgcacgtc acgcatcgca tataaacctt gttcagagat acgctctgct gccgctgccg
    10861 ttttctgggt ttgctgaact tgctgagttt gctgttgtgc cgctaccgga gcattatcag
    10921 cagcccatac agttgaagat gccagaattg ctgtgaccat cgtggccata attaaacgtt
    10981 tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat
    11041 gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacag ttgtagttat
    11101 tatcgcgaga agtattttag tcttacaatt gtaggaatat gcttcctgtc gcagagaact
    11161 attcttatat accacaaaga gatgtcattt attatatcga tgtaaatact gattaattaa
    11221 atgtaaatac agccataaca tcgtaggttc tcagtgaacc attttcagga taactttcta
    11281 tgaaagtaaa cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg
    11341 tttttgcatt ttccatttcg ggtggtggaa gtgagaggag ctataaagag actgaaaaaa
    11401 catcagcgat gacgaccaca cactctacaa aacttcagcc atcacaggcg attttgttta
    11461 agatgagaga agatgcgcca ccattaaacc tcacagaaga aatgccgccc ccttttccga
    11521 caaaggcgaa ttatcttatt catcctgtgc gatagcgtct ttcacatggc aggtgaacaa
    11581 tcatgtctga tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actaccgcct
    11641 gccatacccc accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat
    11701 ccccgtcact agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc
    11761 gaggatcacc atgccgaaga aagaagagac cgccgatacc acgttcagta cctgaccgat
    11821 gtagccgctt acgcagacaa tacacagggc gagaaacatc cataaattaa tcatagctca
    11881 acctcctgcc gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta
    11941 acctctggca atttttgcta agcatcaatg aaaacagcat gttaaatgca agactgttgt
    12001 gtacggaaaa atatttactt tgcacgatta ataatcagta gctgaaagca gtcagcgagt
    12061 gataaagtac agataccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac
    12121 tctttacagg aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac
    12181 atggaatctt ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg
    12241 caaattttcc cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt
    12301 actctggctt tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgtattgg
    12361 cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact
    12421 ttgtttcccg cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc
    12481 actcggcatg gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg
    12541 taaatggttt gatgttgaag ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg
    12601 cgtaatggtt gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact
    12661 gtgccaacca atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca
    12721 gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat
    12781 tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg
    12841 taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac
    12901 ctatgttctc tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc
    12961 ggattacagc ggatatcgtt tgttcatcac cccagagatg gaaggctacc cgacagatga
    13021 aaatcaagcc gctgcctata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga
    13081 gcagtacctc tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta
    13141 catttaacaa actgctctta ctctttccct cctcaaatcg gccataataa cctcatggtt
    13201 tttatggctt ttttcttaag gttttttcct tcgctgaata tctaattatt accgtcagaa
    13261 attaaactgt cgccgatatg agacacttgt aagttactaa aagtaaataa gaaaatgcct
    13321 cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct cgagcagttt agctcagaat
    13381 tctggggtga tgcccacggt aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac
    13441 tcactgtatc aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta
    13501 aagtgaaata tttctttatg ggcatttctt ttatggtcat cgtttgggcc ggtacttttg
    13561 ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg ctcctgtttg
    13621 catttctgac gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag
    13681 taaaccgtcg gcacggaaca tcgctttaat ccctctgatg gcctgacgaa tacggtcgcg
    13741 gttttcaatc agtgcaaagc gaacatgcgt atcaccatag tcgccaaagc caatccccgg
    13801 cgagacacaa accttcgctt cgttaaggag cttcttggca aattccagcg atcccatggc
    13861 cgcatatggt tccgggattt tcgcccagac atacatcgaa gccttcggca tttcaaccat
    13921 ccagcccgct tcatgcagcc ctttaaccaa tacatcacgg cggcgtttgt actgttcggc
    13981 aatgtcgcgc acgcactgtt gatcgccctc cagcgccgca atcgccgcca cctgcaacgg
    14041 cgtaaaggtg ccgtaatcgt gatagctttt aatacgagcc agggcgctga ccagcgtttt
    14101 gttgccaacc ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa
    14161 gaactcgact gccacatcgc gtgcacccgg tacctgcata attgacggcg ctttccagcc
    14221 atcgtagacg atatcggcat aggccaggtc atggaccacc agcacatcgt agcgtttcgc
    14281 cagcgccact actttttcga agaactccag ttccacgcat tgcgcggttg ggtttgacgg
    14341 gaagccgagg atcatcatct tcggtttcgg ataactttca cgaatggctc gctccagttc
    14401 gttgaagaaa tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac
    14461 cgcgccataa atatggatcg ggtaacttgg attcggcacc agcaccgtgt caccatgatc
    14521 cagcgtcgcc agcatcagat gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc
    14581 tgattccggg tcgatttcaa cgtcgtagcg atcctgatac catcgggaaa tggcgcgacg
    14641 taaccgcgga atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccacagt
    14701 acataatttt tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc
    14761 gataatatct tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga tgttaaaaac
    14821 gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat
    14881 ttcctcagat taacgttagc gcccggaccg tccgagcgac gctgccacaa tggtggctgt
    14941 tttgaaaata gcctgattaa tttctgtctg tccagcgttt tagaacattt ttatcaatgg
    15001 ctttaaatga ggaactcaac ttcaaaaaca ggaaattttt ctacgctaac aagaaaatta
    15061 ccaccaattt tattaacatt ataatttcaa taaatttacc atccagaagc gcattatcaa
    15121 gagatacatt gctaatacac catttatctt tcaatcccct tttaaattta tggtttttcc
    15181 tcaaaaacgg cctgctggtc atcagccttc aggttaccta tcatagaggt ttaatcctta
    15241 ttcagagtca cccgtgcacg aaatattcaa catgctgctg gcggtcttcg atcgggcagc
    15301 gttaatgctt atctgcctgt tctttctcat ccgtatccgc ctgtttcgcg aactgttgca
    15361 caagtcggcg cactccccaa aagaattgct cgccgttacc gccattttct cgctgttcgc
    15421 cctgttcagc acctggtccg gcgttcccgt agaaggctcg ctggtgaacg tacgtattat
    15481 cgcggtgatg tccggcggga ttctgtttgg cccgtgggta ggcatcatta ccggcgtgat
    15541 tgcgggtatt caccggtatt taattgatat cggcggcgtg acggcgatcc cctgctttat
    15601 caccagcatt ctggcgggtt gtatatcggg ctggatcaac ctgaaaatcc ccaaagcaca
    15661 gcgctggcgc gtcggtattc tcggcggcat gttgtgtgag acgttgacca tgattctggt
    15721 aattgtctgg gcaccaacta ccgcgttggg gatcgatatc gtctctaaaa tcggcattcc
    15781 aatgatcctc ggtagcgtct gtatcggctt tattgtgctt ctggtgcaaa gcgttgaggg
    15841 cgaaaaagag gccagcgccg cgcggcaggc caagctggcg ctggatatcg ccaacaaaac
    15901 gctaccgctg tttcgccatg tcaatagcga gtcattacgc aaggtctgcg aaattatccg
    15961 cgatgacatt cacgccgatg cggtggcgat tactaatacc gatcatgtgc tggcctatgt
    16021 tggcgtgggt gaacataact atcagaatgg cgatgacttc attagcccga ctacccgtca
    16081 ggcgatgaat tacggaaaaa tcatcattaa aaacaatgat gaagcccacc gcacaccaga
    16141 gattcattcc atgctggtga tcccattgtg ggagaaaggg gtcgtgaccg gaacgctgaa
    16201 aatttactac tgccacgcgc atcagatcac ctcgtcatta caggaaatgg cggtcggtct
    16261 gtcgcaaatc atctccacgc aactggaggt ttcacgcgcc gagcagctac gtgaaatggc
    16321 aaataaggca gagcttcgcg ccctgcaaag caaaattaat ccccattttc tgtttaacgc
    16381 tctgaacgct atttcatcgt caatccgtct gaatccggat accgctcgcc agttgatctt
    16441 taatctgtcg cgttatctgc gctataacat tgaattaaaa gacgatgagc aaatcgatat
    16501 caaaaaagag ctgtatcaaa ttaaagacta tattgccatt gagcaggccc gctttggtga
    16561 caagctgacg gttatctatg atattgatga agaggtgaat tgctgcattc ccagcctgct
    16621 gatccagccg ttggtggaga acgccattgt ccacggtatt cagccttgca aaggtaaagg
    16681 cgttgtcacc atcagcgttg cagagtgcgg aaatcgggta cgcattgcgg tgcgagatac
    16741 cgggcacggc atcgatccaa aggtgattga gcgggtcgaa gcgaatgaaa tgccgggcaa
    16801 taaaattggc ctgctgaatg tccatcatcg cgtgaagtta ttgtatggcg aggggctgca
    16861 tatccgccgc ctggagccgg ggacggaaat tgcgttttac attcctaacc aacgcacccc
    16921 agtcgcctca caggctacgt tattgctttg agccggagtg atattgtgaa agtcatcatt
    16981 gttgaagacg aattcctggc acaacaggaa ctgagctggc taattaaaga gcacagccag
    17041 atggagattg tcggcacctt tgacgacggt ctggacgtgt tgaagttttt gcagcataac
    17101 cgcgtcgacg ccatttttct ggatatcaat attccgtcgc tggatggcgt gttgctggcg
    17161 caaaacatca gccagttcgc ccataaaccg tttattgtgt tcatcaccgc gtggaaagaa
    17221 catgcggtag aagcgtttga actggaggcg tttgactaca ttctcaaacc gtatcaggag
    17281 tcacgtatta ccgggatgct gcaaaaactg gaagcggcct ggcaacaaca gcagaccagc
    17341 agtacgcctg ccgcgacggt aacgcgtgag aatgacacca ttaatctggt gaaagatgag
    17401 cgaataatcg tcacgccaat taacgatatc tattacgccg aagcgcacga gaaaatgacc
    17461 tttgtctata cgcggcgtga atcctacgta atgccgatga acattaccga attttgcagc
    17521 aaactgccgc cgtcgcattt tttccgctgc catcgctcat tttgtgtcaa tctgaacaaa
    17581 atacgcgaaa tcgaaccgtg gtttaataac acctacattc tgcgactgaa agatctggat
    17641 tttgaagtgc cggtcagccg cagcaaagtg aaagaatttc gccagttaat gcatctttaa
    17701 tcgggaactt tcatgaaagc accaggcttg cccgccgatc aacaattttt cgccgatctg
    17761 ttcagcggcc tggtgcttaa cccgcaacta ctggggcgcg tctggtttgc cagccagcct
    17821 gcctcgttac cggtgggcag tttatgtatt gattttcccc gtctggatat cgtgctgcgc
    17881 ggcgaatacg gcaacctgct ggaagcaaag cagcagcgaa tggtggaagg agaaatgctg
    17941 tttattccgg cacgcgcggc taatttaccg atcaacaaca aacaggtgat gctgttaagc
    18001 ctggtgttcg caccgacctg gcttgggtta tcgttttacg atagccgcac cacgtcgttg
    18061 ttgcatcctg ctcgccagat ccagcttccc agcctgcaac gcggtgaagg tgaagcgatg
    18121 cttaccgccc tcacccatct tagccgttcg ccgctggagc agaatatcat tcagccactg
    18181 gtgttaagtt tgctgcatct ttgccgtagc gtggtgaata tgccgccggg taattcgcaa
    18241 ccgcgcggcg attttctcta tcacagcatt tgtaactggg ttcaggataa ttatgcccag
    18301 ccgctcaccc gcgagagcgt ggcgcagttt tttaatatca cgcccaatca tctgtcaaaa
    18361 ctgtttgctc agcatggaac gatgcgtttt atcgagtatg tgcgttgggt gcgaatggcg
    18421 aaggcgagga tgattttgca gaaatatcat ctgtcaattc atgaagtggc acagcgttgc
    18481 ggttttccgg acagcgacta tttttgtcgc gttttccggc gtcagtttgg tctgacgccg
    18541 ggagagtaca gcgcccgttt tcagggctaa cgtcagaagg ttaattccgt ttccagcagc
    18601 gtcaggatgc tttgcgcatc ttgcgcggca aacaatgact ggcggaagtt tttattcacc
    18661 agtttacgcg ccagctgcga gaagactttc acatggttca tcccttcgtt agcacccaac
    18721 gtcagcatga tcaccagttc gacttcgccc atctctgact gccagtcaac cggtttcacc
    18781 agccgggcaa tgctgatact ggaatgacgg atccactgag atttggtgtg cgggatcgcc
    18841 acgccaaaac caacgccggt agtgacaatc tcttcccgct gccagacatc ttcttccagc
    18901 tcaaacggat gttcagtacg cccgttaacg ccgaggttgc cgcacaggaa ctggatcgcc
    18961 tgctctttat tgctaaaatc ctgatcaaca aagatattct ccagcgccag cagtgggcga
    19021 acgtcttctt ccggcgtaaa ggcggtgagt aacgcttcaa tttcctgcgc actgcggcat
    19081 tcacatgcct gacgcgccag ttcccgacac gcctcgctat ccagttgacg aagctggctt
    19141 ttcactgccg gaatgcgcgg gctactcata ctcagctcat ccaggcccag cccaagcagt
    19201 agcggtagat aacgactttc accgcccagt tcaccgcaaa tacctaccca tttgccgcgc
    19261 tgatgcgcag tggtaacaat ctgctgcaac atgcgcagga acgatggcgt aatcgggtta
    19321 tacagcggcg atacgcgcgg gttattacga tcgaccgcat acagatactg ggtcatatcg
    19381 ttagaaccga tactgaagaa atcgacctca tcgcagaagt ggtcgatgat gtaacacacc
    19441 gacggcactt ccaccatgat acccagagta atcgtctctg catgacgcag gccatcgcgc
    19501 ttaagctcaa cgatcgcttt ttggatctcg cccttcaccc ataagatctg atcgaggctg
    19561 tgaaccatcg ggatcatcaa ctgggcgttg ccaaaactgg cagcacgcaa aatcgcccgt
    19621 agttgagtgc ggaacaggcc agcaaattcc gggtaaatac gtaccgcgcg atagccgagg
    19681 aacgggttct cttcctgggg aatattcaga taaggaatgc ttttatcgcc tccgatgtcc
    19741 attgtgcgga agataatcgg cttgtcgccc gctgccagta gcacctgctg gtaggcttca
    19801 aattgctcct gctcgtccgg cgcgctgtca cgatccatat acagcatttc ggtacggaac
    19861 agcccgacgc cttccgcgcc gttggcaaac gcgcctggtg cttccagagc ggtgccaata
    19921 ttcgccgcga tatcaatacg cttgttatca cgggaatagg ccagctgcgc ggcagcctgc
    19981 gcctgttgtt tttggcgttt atccgccagc gtctgcgcaa cctgataata accgctcacc
    20041 gcgtcattcg ggttaatcgc cagcacgccg cactgggcgt caagcaccgc cggttgcccg
    20101 gcataacggg caatcgcatc cagcggcagg ccgctcagta ccgggatcgc cgaggcgcgg
    20161 gccagaatca gggtatgcga ggtgcgcccg gttttctcca gaatcatgcc cgcaagattt
    20221 ttcaaatcaa ggctcaaaaa ctggcttggg gttaaatctt cagccaccag aatggtcggt
    20281 ttttccagca ccagattgtt gcgcggcttc agttccggcc aggtgatatg cagcaactgt
    20341 tcgctaatat cgcgaatgtc gctaacacgt tcgcgcagat aatcgctggc agaagcagaa
    20401 agcttggcgc aaacctgctc catattgctg atgatcgccg cccccagtcc ctgatgctgt
    20461 tctgtcatca ggcgacggat attgcctgca aattcatcat cctgaatcag cgacaaatgg
    20521 gcgctgagga tagttttgct ttcgccgtca cgctcacgca attgctggtt cagttgctcg
    20581 gcaagcgttg ccaggctgtg ctccagccgg gtggaatctt gcacactggc ggggattgcc
    20641 cgataactgt cgaggctgtc gctttgtaac agggtcagcg tacccacgcc gacgccgctt
    20701 gccagcacat tgccgtacag taaatccggg ttaaggcggc ttaatgaacg cggcagcgga
    20761 tgcgccgtca gttctgcctg cgtaggctgg acgctgtcgc tgtcgataaa gcgcacctgg
    20821 atgtactctt ccagcacgcg ccgcgcctgc tcttcatcgc tgccgctaat gttcaggctg
    20881 caactgtcat taaatagggt gccggtgcca atcagcgcca gcgaactttt cgcatcggcc
    20941 tttgcgttct ggcgatggtt aataaaggtg acttcacttt gccactggct gcactgttct
    21001 ttaagttccc acgccggacg agcatgtaga ccgttaggca aaggacagag aaattgaatc
    21061 gttaacataa ccgctccggg ttaatcagga gatcatctga aatccgtcag ttgaacaacc
    21121 gtctcacgcg taagacgttg aataagggca gataaaagtt gctgcatctg caaaatgtcg
    21181 cggcaatcgg caatcgatgc ggcgcaatgt ccatggcggg ttgctggccc catcaccact
    21241 gtgggcacgc cggtgccggt taagtgcacc gccccgccgt ccgtgccgcc gttgctgaac
    21301 atatctgcct gcaacggcac gccaatttct gccgccacgg tttcgaccca ggcggtaagt
    21361 tttggcggcg caatcagcga cttgtcgctt aacaccagca tcggcccgtt accaatctgg
    21421 cgatggttag ccgcgccata atcaaagttt ttcgcccagc aggcggtatc aagcacaatg
    21481 gcgacgtccg gcgacaccgc gcgggtggca gtttgcccgc cgcgtaatcc cacctcttcg
    21541 ctggaacttg ccaccagcca cacttccgca ggtagttcgg cgtcgtgcag ttcgcgcagt
    21601 aacgtcacca gcagatagca accgaggcgg tcatcaaagg ctttccccat cactcgctgg
    21661 tgagggagaa cctgaaaagt ggtatcaaac gtgacgcgat cgccgggacg aattcccgcc
    21721 tgcatcactt cgtcatagga gcgcgcacca atgtccacgc gcatggcgct gacgtcattc
    21781 ccctgccggt cgccgtcaag caggcctgga attttgcact cttcacgggt ggtgatgcgc
    21841 accggctgca gctggcgggc agccatgcgt acgttgccaa ccggcagcac atcaatcgcc
    21901 ccttcgcggg agatgctgcg caccataaat cccacttcgt ccatatgcgc acagatcatc
    21961 accttcggac ctgtcgattc attcaggcgg atcagcaccg atcccagacc atcaaatcgc
    22021 acttcttttt gcaggcgatc cgcttcttcc agcaggatct gccgcacttc ctgttccgag
    22081 gaggcgatcg catctgcctc gctcaacgct tttaatagcg ataaatccat tatgcctctc
    22141 ccgtgagcaa cactgttttc ggcatggcgt agagcacttc tgcgccttgc ggggtaacca
    22201 gcacaacatc ttcgatgcgc acgccccctt gccctggcaa ataaatcccc ggctccacgg
    22261 tcagtaacat gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc ggatcttcat
    22321 gaacttcaat gccgatagcg tgaccggtgt tatgaccgaa atagtcgcca taacctgctt
    22381 ctgtaatgac ccggcgcgcg gcatcgtcaa cctgctggca gcgcacgccg gggcgaatcg
    22441 cggagattgc tgcgagctgt gcctgcagga caatttgata gacgttaaac agcgggtgag
    22501 attcggcgct caccccttcg ccattcacca gcaaggtgcg cgtcatatca gagcagtagc
    22561 cctgatatag cgcaccgaaa tcgagagtga caaactcgcc cgctgcaaca atcttgtcgc
    22621 tggctttgcc gtgcggcagc gccccacgcc agccactgac gacaatggtg tcaaaagagg
    22681 ctttttctgc gccctgctgg cgcataaacc actccagttc agcggctatc tcgcgctcgc
    22741 tcatccccgc ctgaataaag cggcgaatat gctctgcacc gcgatcagca atcccacagg
    22801 cgaggcggat tttctccacc tcctctggcg ttttgatttg ccgcagcaca tccggcgtgg
    22861 cgctaaccag tttcgcattg agttcagact gccagcggtg cgcggtttcc caactcacct
    22921 gctggccctc aaaaccgagc gtttgcaact gttcatcggc aatgatttga ttgacgatag
    22981 tggtaagcgt gttcgtcgcg tcaagcaaat gcagctggta gccttgcgca cgggcttcca
    23041 cctcaacgta atagcgcgaa tccaccagaa tgtgcgcact ttcacggcta atcaccacat
    23101 aacctgatcc ggtggagatc cccagatgcg gctgtttgtt ctgccgtgag gagagaagca
    23161 ctgcatccag ttgttgcgcc ttaagccagt cgcgcagcga agcgagtaat gtcatctgga
    23221 ttttccttct tacaggctat cgattaacaa tttgccttta cggaacatca tcagacgcag
    23281 gaacaccacc atcagcgcag ttatgactgc ccccagcgcg atccccgcca tatagacgcc
    23341 caggttagtg accagcggcc atgcccagat agcggactcc gggaaccatt gcactgcgcc
    23401 cagccagaca gcggcggttg agccgacaat agcgccgacc atatacgacg gaatggcggt
    23461 gatggggctt tccagcgcaa acggaatcgc accctcactg atccccataa acgccaggaa
    23521 catggcggtc ttaccctgcg gataaagttg agcgttgaac aggcgtttgc cggttaaacg
    23581 acggtcgata atggtcgcca gccccagacc aatcggcgga ataacgatag cgatagaacg
    23641 agcggtgacc ggtaatacgt ggtcagtggt aaagctgaag gcaacaaaac ctgcggcttt
    23701 gttgatcggg ccaccaaggt cgattgctgt cgccgcggcg atccccattg cgtacatcag
    23761 cgcgcccttc tcacctgcgg cagtcagcac agtacggata ccgccgttga tccagccacc
    23821 aaagggggtg atgacgtagt acatcgccag catgacaaaa atggcggaaa gaatcgggag
    23881 caaaaatgtg gttttgaacg ctaacaggaa atccggtaac tggattttct ggttcatcca
    23941 cttcacaagg taacccgcga cgatagaaat aatcagcgca ccaatgaagg tggacggcac
    24001 cggcgaagag gtcgcccatt gcatagtgct gggatcgaag ttcagcagtt gcgtcggctg
    24061 ggtagacatc aacccgccga taaaaccagc cgggaacgcc agtttgccac cgatagagtt
    24121 cgccacaaaa gcagcaaaca tcggaatggc aaaaccaaac aacacgccgc cgaaggactg
    24181 cgacagccag gcaaatttaa gtaaggagag gtcgaaaccg gagaatttcc cgctattaag
    24241 agcatccatg atgccgatct cagcaggaat tttaagccag ctataagcaa tcagctggct
    24301 gaaagcgagg atcacaccgc ccataattaa ggtcggcacc atgcgtgaaa tccctgacat
    24361 cacatgctgc gggagttcac cccagaagct ggttttagag gcctgcggat tcttaactgc
    24421 cgctgccgca ccggatgcgc caggcacaac ggttgcactg cgttttttaa tggccataac
    24481 aattatccct gtcgaatatt actgttgttc agaggcaatc atctcttcga tttctttgat
    24541 gatgcccgca gcgtttttaa ttgcgtcctg caaagtgatt tcataaacgt cgcgtgattc
    24601 gaaacgttcg ttatcttccg gggtaactgc cacggagtgg atgatgatgg tcgcttcggc
    24661 gatatcctgc gccgtcaggc gattctggat accgtccgcg ccctgggttt caattttcac
    24721 ttcataaccg gcttctaccg ccgcttcttc cagcgcctga gcggccataa aggtgtgagc
    24781 caggcccatc gggcaggcac ataaggcaat cagtttctta ctcatcgtta attcctcgtt
    24841 cagtgatagt tgacgagtga aattatgaac gtgcaaaaca aatcgccgtt accagacaaa
    24901 taatgcattt actggagaat taatcccgct gtacggaagt gtgacgcagg tcggccttgt
    24961 gaggggacac aaagaaaaat tgaataaact gtatgattta aaagattatc gggagagtta
    25021 cctcccgata taaaaggaag gatttacaga atgtgaccta aggtctggcg taaatgtgca
    25081 ccggaaccga gaaggcccgg attgtcatgg acgatgagat acaccggaat atcatggaca
    25141 tattctttaa agcgcccttt atcttcaaat gcggcacgga aaccggaggc tttgaagaac
    25201 tcaaggaagc gcggcacgat accgcccgca ataaacacgc cgccaaatgt cccgagattg
    25261 agcgccagat tgccgccaaa acggcccata atgacgcaaa acagcgacaa tgcgcggcgg
    25321 caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat cttttggctt gagattttct
    25381 ggcaggcggt tgtcagcttt cacaattgcg cgatacaaat tcaccagccc agggccagaa
    25441 agcacgcgct ccgccgaaac atgaccaatt tccgcacgca atatttcgag gataatggcc
    25501 tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt cgcctggcaa gcttacccaa
    25561 cgcttatcga catggaccag atgcgcaacc ccaagccccg ttccggcacc gtaaaccgca
    25621 ataggcttac cttcgaccgg ttctgcgcca ccaaactgaa tcagatgctc ttttttcagc
    25681 atcgggatcg ccatcgatac agcggtaaaa tcgttaataa tttccagatg gctaaaaccg
    25741 agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat cgccacccag
    25801 tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac cttatgttct
    25861 tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga ataggtctta
    25921 gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt ggtgccgccc
    25981 acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag tcaaaataat
    26041 tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg caggctccct
    26101 gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt gcagcgatgc
    26161 ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga acatgctcca
    26221 tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg ggattgcgtc
    26281 cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta ttacagaatt tgctctggca
    26341 acgactgccg ggaactctgg ggatagccca ggattcaccc ctctggatca tcggtgtatt
    26401 aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc atgccggacc
    26461 agaccccgcc tgtgaaccgc tgatcggcgc accggttccg ccctctgcgc tacctggact
    26521 tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg aacatccgat
    26581 catgaccgtc aatatcgccc ttgcggttgc cattggcgct cgtctgttac cgcgcgtcaa
    26641 ccgaatggag tggactattt tagcctctgc cggaaccatc ggcgcgctgt ttggtacacc
    26701 tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc agtagtgaag ttccgctatg
    26761 ggatcgtctt tttgcgccgt taatggcggc agcagctggt gcacttacta ccggattatt
    26821 tttccatcct catttttcac tgcccattgc tcattacgga cagatggaaa tgaccgatat
    26881 tctcagcggc gcaattgtcg cggcgattgc catcgccgca gggatggttg ccgtatggtg
    26941 cttaccacgg ttgcacgcga tgatgcatca aatgaaaaat ccggtgctcg tgctgggtat
    27001 tggcggattt attctcggta ttctgggggt tattggtgga ccagtttcgc tgtttaaagg
    27061 gctggatgag atgcagcaga tggtggcaaa tcaggctttc agcaccagcg attacttttt
    27121 gctggcggta attaaacttg ccgccctggt cgttgctgcc gccagtggct ttcgcggtgg
    27181 gcgaattttc ccggcagtgt ttgtcggcgt ggcattaggg ttgatgctgc atgagcacgt
    27241 tcccgccgta ccagcggcaa taaccgtttc ttgcgctatt ctcggcatcg tgctggtggt
    27301 aacacgcgat ggctggttaa gtctttttat ggcggcagtc gttgtaccca ataccacatt
    27361 gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg ttattagcag gtaagccgat
    27421 gatgatggtc aatcgtccga agcaacagcc cccccacgat aacgtttagc aaaaaatgct
    27481 ctcctttatg atttaagagt tatggcgatg atacggggct ttacctcccc gtaatattgc
    27541 gttaacaggc cgctgacggc ttcgatcgtg aaggagaata acaatgttca ggtcactgtt
    27601 tctggcggcc gccctgatgg catttacccc gcttgcagca aacgcaggtg aaatcaccct
    27661 actgccatca atcaaattac aaattggcga tcgcgatcat tacggtaatt actgggacgg
    27721 tggtcactgg cgagatcgtg attactggca tcgcaattat gagtggcgca aaaaccgctg
    27781 gtggcgtcat gataatggct atcatcgtgg ctgggataag cgtaaagcgt atgagcgtgg
    27841 ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga aaaggccgcg gacatggaca
    27901 ccgccattaa acacattcgc caatggagca caatgcctga tgcgacgcta gcgcgtctta
    27961 tcaggcctac aaaccggctc tttaacgcgt agggcgggta aggcgtttac gccgcatccg
    28021 ccaatagtgc cagatgcgac gctcattcaa ctacaatccc agtgccgtac ccaccaacaa
    28081 ccagatattc agcgccacta ccagcaccac aatcacccag cctgtctgtt ttacgcgttt
    28141 gctgttcacc agatcgccca tcaacttgct gtcactggtg aaaatcagca gtggaaccag
    28201 cgccagagcg ataccaaaac ttaacagtac ctgactcata accagaatcc gtgtcggatc
    28261 taatcccatc agaatgacaa taaatgacgg caacatggtg actgtacgac gcacccacag
    28321 cgggatatga aagcgaatga agccctgca
//
LOCUS       DAZFZC010000068        28275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFZC010000068 DAZFZC010000000
VERSION     DAZFZC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.068"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..120
                     /gene="yqcG"
                     /locus_tag="ACHKXP_003217"
     CDS             <1..120
                     /gene="yqcG"
                     /locus_tag="ACHKXP_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFO7648169.1"
                     /translation="NGFNHVKTFTKIIFIFSVLVFNDNEYKITDAAVNLFIQI"
     gene            134..1006
                     /gene="ygcG"
                     /locus_tag="ACHKXP_003218"
     CDS             134..1006
                     /gene="ygcG"
                     /locus_tag="ACHKXP_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO7648170.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1066..2364)
                     /gene="eno"
                     /locus_tag="ACHKXP_003219"
     CDS             complement(1066..2364)
                     /gene="eno"
                     /locus_tag="ACHKXP_003219"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO7648171.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2452..4089)
                     /gene="pyrG"
                     /locus_tag="ACHKXP_003220"
     CDS             complement(2452..4089)
                     /gene="pyrG"
                     /locus_tag="ACHKXP_003220"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO7648172.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4317..5108)
                     /gene="mazG"
                     /locus_tag="ACHKXP_003221"
     CDS             complement(4317..5108)
                     /gene="mazG"
                     /locus_tag="ACHKXP_003221"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO7648173.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5179..5514)
                     /gene="mazF"
                     /locus_tag="ACHKXP_003222"
     CDS             complement(5179..5514)
                     /gene="mazF"
                     /locus_tag="ACHKXP_003222"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO7648174.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5514..5762)
                     /gene="mazE"
                     /locus_tag="ACHKXP_003223"
     CDS             complement(5514..5762)
                     /gene="mazE"
                     /locus_tag="ACHKXP_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO7648175.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5840..8074)
                     /gene="relA"
                     /locus_tag="ACHKXP_003224"
     CDS             complement(5840..8074)
                     /gene="relA"
                     /locus_tag="ACHKXP_003224"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO7648176.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8122..9423)
                     /gene="rlmD"
                     /locus_tag="ACHKXP_003225"
     CDS             complement(8122..9423)
                     /gene="rlmD"
                     /locus_tag="ACHKXP_003225"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO7648177.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9480..12236
                     /gene="barA"
                     /locus_tag="ACHKXP_003226"
     CDS             9480..12236
                     /gene="barA"
                     /locus_tag="ACHKXP_003226"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO7648178.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12469..13809)
                     /gene="gudD"
                     /locus_tag="ACHKXP_003227"
     CDS             complement(12469..13809)
                     /gene="gudD"
                     /locus_tag="ACHKXP_003227"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO7648179.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13830..15170)
                     /gene="gudX"
                     /locus_tag="ACHKXP_003228"
     CDS             complement(13830..15170)
                     /gene="gudX"
                     /locus_tag="ACHKXP_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO7648180.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGDVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQREAITVLGYLFYIGDRTKTD
                     LPYVENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15172..16524)
                     /gene="gudP"
                     /locus_tag="ACHKXP_003229"
     CDS             complement(15172..16524)
                     /gene="gudP"
                     /locus_tag="ACHKXP_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO7648181.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNKKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16959..17408)
                     /gene="yqcA"
                     /locus_tag="ACHKXP_003230"
     CDS             complement(16959..17408)
                     /gene="yqcA"
                     /locus_tag="ACHKXP_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO7648182.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17426..18208)
                     /gene="truC"
                     /locus_tag="ACHKXP_003231"
     CDS             complement(17426..18208)
                     /gene="truC"
                     /locus_tag="ACHKXP_003231"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO7648183.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18208..18537)
                     /gene="yqcC"
                     /locus_tag="ACHKXP_003232"
     CDS             complement(18208..18537)
                     /gene="yqcC"
                     /locus_tag="ACHKXP_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO7648184.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19159..19704)
                     /gene="syd"
                     /locus_tag="ACHKXP_003233"
     CDS             complement(19159..19704)
                     /gene="syd"
                     /locus_tag="ACHKXP_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO7648185.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19772..20620
                     /gene="queF"
                     /locus_tag="ACHKXP_003234"
     CDS             19772..20620
                     /gene="queF"
                     /locus_tag="ACHKXP_003234"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO7648186.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20732..22096
                     /gene="ppnN"
                     /locus_tag="ACHKXP_003235"
     CDS             20732..22096
                     /gene="ppnN"
                     /locus_tag="ACHKXP_003235"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO7648187.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22653..23942
                     /gene="sdaC"
                     /locus_tag="ACHKXP_003236"
     CDS             22653..23942
                     /gene="sdaC"
                     /locus_tag="ACHKXP_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO7648188.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            24000..25367
                     /gene="sdaB"
                     /locus_tag="ACHKXP_003237"
     CDS             24000..25367
                     /gene="sdaB"
                     /locus_tag="ACHKXP_003237"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO7648189.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIAHNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25479..26234
                     /gene="xni"
                     /locus_tag="ACHKXP_003238"
     CDS             25479..26234
                     /gene="xni"
                     /locus_tag="ACHKXP_003238"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO7648190.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26289..27437)
                     /gene="fucO"
                     /locus_tag="ACHKXP_003239"
     CDS             complement(26289..27437)
                     /gene="fucO"
                     /locus_tag="ACHKXP_003239"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO7648191.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27465..28112)
                     /gene="fucA"
                     /locus_tag="ACHKXP_003240"
     CDS             complement(27465..28112)
                     /gene="fucA"
                     /locus_tag="ACHKXP_003240"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO7648192.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6885 a         7518 c         6754 g         7118 t
ORIGIN      
        1 aatggattta atcatgtcaa aacattcacc aaaattatat ttattttttc tgtattagtt
       61 tttaatgata acgaatataa aattaccgat gccgccgtca atttatttat ccagatttaa
      121 tgagaaattt ataatgcgct atttcatact aatgttcact ttcgtatgtt cctttgttgc
      181 agcccaacca actattgtcc cacaattaca gcaacaagtt acggatctta cgagtagctt
      241 aaactcacaa gaaaagaaag aactgacgca caagttagaa tctattttca ataataccca
      301 agtgcaaatt gccgtattaa ttgttcccac aaccaaagac gagacaattg aacaatatgc
      361 tacaagagtt tttgacaatt ggcgtttagg agatgccaaa cgtaatgatg ggatactgat
      421 cgttgttgcc tggtcggatc gcactgtccg catccaggta ggttatgggc tggaagaaaa
      481 ggtaaccgat gctctggctg gagatatcat ccgtagcaac atgatacccg catttaaaca
      541 acaaaaatta gctaagggat tagagctagc tataaacgct ttgaataacc aactcacttc
      601 acaacaccaa tatccgacta atccttcaga aagtgaatca gcgtcttcca gtgatcatta
      661 ttactttgct attttttggg tatttgcagt gatgttcttc cctttctggt tttttcatca
      721 aggcagtaat ttttgtcgcg catgtaaaag tggagtctgt atttcagcaa tctatctttt
      781 agatttattc ctgttctccg ataaaatttt ttccattgct gtattttcct tcttttttac
      841 tttcaccata tttatggtct ttacctgttt atgtgtactt cagaaaagag catctggtag
      901 aagttatcat tcagacaaca gcggttccgc tggaggctca gatagcggcg gatttagcgg
      961 cgggggcggt tcttctggag gtggcggcgc atccggccgc tggtaactca accataaaaa
     1021 atgccagccc ggaggctggc atttttaaat cagataaagt cagtcttatg cctggccttt
     1081 gatctcttta cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag
     1141 ctggttgtat ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc
     1201 agcagtacca acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga
     1261 gataactgca gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag
     1321 agaaccgatc tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc
     1381 tttcaggatc ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc
     1441 cagaactttg gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat
     1501 agaaacgatc gggtactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt
     1561 gaacgctttg ttgccttcgc cagccagaac gtatttacca tctttgtaga attcagaagc
     1621 tgcgcagtcc atcgccaaag tgatgtcttt gcccagttca taaccagcag ctttaacagc
     1681 ttcagcgata acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc
     1741 gtcaccaaca gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac
     1801 ttcagaaccc atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa
     1861 ttcctggata tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg
     1921 aaccggcata gagtatttgc ccggagtacc gttcagttca gcgatgtgct cgtacagcgg
     1981 cataccttta gcagctgcag cagctttggc gttagccaga gatacagcca ggattgcgtt
     2041 cgcgccgaat ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc
     2101 agcctgatct ttagcatctt tgccaatcag cgcctgagcg atcgggccgt ttaccgcagc
     2161 aacagctttg gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag
     2221 agcttcacgg gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc
     2281 ctccagatgt acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc
     2341 gatgattttt acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc
     2401 cagacaaaca acgcgtaccc agggtacgcg ttgccgctct aactttttta cttacttcgc
     2461 ctgacgtttc tggaactcgc tggcggcttt cacaaagcct gcaaacagcg ggtgaccatc
     2521 acgtggagta gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg
     2581 aacttcgatg atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc
     2641 atcttcaatc tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac
     2701 aattgtcggc gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg
     2761 tgcgccgaga cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc
     2821 gttttcatcg cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa
     2881 ttccgtagag ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac
     2941 ctgcataccc aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt
     3001 cgtaatcatg ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag
     3061 acctttaagg atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt
     3121 gacgctgaca cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc
     3181 atccggcagt tcaatgtact tgccgaccat accgatggtg acttcactta ccgggttcgc
     3241 ttcttcgaag ataacctgtt cccattcgga cagattcgct tccgggcagt ttaagctgaa
     3301 tcgtttacaa atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat
     3361 ggaatcgacg tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat
     3421 cttcgcacgt tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg
     3481 gataccgatg gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc
     3541 agacgctgcc atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat
     3601 ttcaacagcc atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt
     3661 accgccgatt tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt
     3721 gattgcgtta gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg
     3781 ttctttacgc agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat
     3841 tttggtacga atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc
     3901 agtaacgaac acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta
     3961 aggatccagt ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga
     4021 ggctgcggca atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt
     4081 cgttgtcatg ctgaacctga gaagttaggt tgaaaagacg atggaataac caagacggga
     4141 aagcagtata cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag
     4201 cgcactattg aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa
     4261 atcaagcgct tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag
     4321 agatcaattt cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca
     4381 cctgtcattt ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg
     4441 aatttttcgt tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga
     4501 ttaaccgtgg caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca
     4561 gcctgccgcg cttcgtacat cacctcgtcg atctcttcgt agactttatc gactaccgga
     4621 ccaagcgtcg tccaatcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcacgc
     4681 attaaagccg gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg
     4741 cgctcttcgg ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa
     4801 ctatcagcaa aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata
     4861 tcattaaagt caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat
     4921 agcagatcgc ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc
     4981 acttcgtagg tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc
     5041 cacgggcagc cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt
     5101 tgattcattg aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat
     5161 agaagtgagt tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa
     5221 ttcctctggg gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt
     5281 tacctgatca gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg
     5341 atatcctttt gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta
     5401 catgaaagga ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg
     5461 gtcaaaatca acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag
     5521 acttccttat ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg
     5581 tcgttgacca gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat
     5641 ttgccatcca ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt
     5701 aacgtagccg ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc
     5761 ataacccttt cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta
     5821 gatacgagca aatttcggcc taactcccgt gcaaccgacg cgcgtcgata acatccggca
     5881 cctggttgag tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca
     5941 tgtcgatggt cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca
     6001 ccttctcgtt ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcta
     6061 ccacgcggac caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa
     6121 tgcgttctgg cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg
     6181 aaataccgcg cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc
     6241 agcgcgcgat gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt
     6301 tactgcggtt ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct
     6361 gctcttcggc actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac
     6421 ggatatcgcc gccaccaatc gccgccagca actcgtcgac atcattgaag ttgtaacgcg
     6481 gcagcagatg tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa
     6541 ggatttgccg cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa
     6601 ttttcgaacg cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc
     6661 tggggttcgg ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg
     6721 tgaacggcac aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt
     6781 ggatgtggta agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt
     6841 tcggcgtaaa gacgtacacc cggtcgtcaa agacctgact acgtacttcg tcgagcattt
     6901 cgccggaatc agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt
     6961 cttcatgtcc cgaacgtgcg ccgccagcag ccgcgccctc tttatatttc cagtgcgcag
     7021 caacacccaa ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt
     7081 ttccacccgg ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag
     7141 cgacgtaatc gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg
     7201 cggcatagca atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct
     7261 catcaaaggc gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac
     7321 gaccatacac ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga
     7381 actcttcgat gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt
     7441 tgtattcggt tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc
     7501 cgattccgag acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta
     7561 cacgttcatc ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt
     7621 tgatgactac gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga
     7681 cctgttcgga ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat
     7741 cacgcacgcc gtgaataagg ttaacgaccg acttaccgac gctctcacgc agcacatctt
     7801 cgctgactac gttggcatcc gccagaggga aaagcagcgc cgcccgcagc gtgtcaatgt
     7861 ccatacttaa tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg
     7921 catccggatg cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac
     7981 acgacttctg gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag
     8041 ccttattgat atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct
     8101 gccgaaatcg gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg
     8161 tcccgtgtgt gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa
     8221 taacgcttcg ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat
     8281 aggttccagt tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag
     8341 caacacttta tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt
     8401 ttcgtgataa aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc
     8461 caccagcgcc ggaacacctt ctacaccgac cacactggca gcttgtgtcg ccaatggcag
     8521 tgtaaagttg cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag
     8581 ccattccaac gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc
     8641 gcgcgggcta aaagttaagc gcaacccgtt tgagtcatac cagggcatct caccagagac
     8701 ggtttcgagt atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa
     8761 gcgttccagt ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag
     8821 cgtgccgctg gttgcctgta ccagttcaac atgaccaaga tggcgcatag cttgtaagct
     8881 gcccagacat gccctgactt tgggcagcaa tgcttcaagt tggggcgcta aaatggggca
     8941 ttgtttgacg tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg
     9001 tgttttcggt aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc
     9061 ggcgatcact tcagagacat cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg
     9121 ctgctgtaaa tccacgctgg cgtgttgttg ctgacagcca ccgcatacgc caaaatgagg
     9181 acagcgtggc gtttcgcgtt ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc
     9241 atactgtttt ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg
     9301 gataaatagc gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt
     9361 gactgaaacg gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc
     9421 cattggcgag actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca
     9481 tgaccaacta cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta
     9541 ttggtttatt gctgagtatc tttttcgtcg tgcatcgcta taacgacttg cagcgtcaac
     9601 tggaagatgc cggtgccagc attattgagc cgcttgcagt ttctactgaa tatggcatga
     9661 gcctgcaaaa tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg
     9721 atattgttcg cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt
     9781 ttcatcttga tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca
     9841 ctgtcactcg tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact
     9901 cccccgacga atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg
     9961 cgctggagct ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca
    10021 gcgtgatgat gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg
    10081 atgtaaccgg tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac
    10141 tcgacagccg ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca
    10201 actcgatggc aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg
    10261 cgacgtccga tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc
    10321 tggcgaaaaa gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt
    10381 cacacgagct gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa
    10441 cagaattaac accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt
    10501 tgctggcaat tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc
    10561 tggaaagtat tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac
    10621 attcttctca cgataaaggg ttagaactga cgctcaatat taaaagcgac gtgcctgata
    10681 acgtgatcgg cgacccgctg cgattacagc aaatcatcac taacctggtg gggaatgcaa
    10741 ttaaattcac cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata
    10801 ccaaagtgca gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc
    10861 aatcgcgctt attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg
    10921 gcaccgggct ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt
    10981 cgttccatag ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga
    11041 acccgaacat tattatcgaa gggccatcca cccagtgcct cgcaggtaag cgcctggcct
    11101 atgtcgaacc aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc
    11161 tggaagtggt ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt
    11221 tgttaggcat cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga
    11281 aagcggtatc gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg
    11341 ctgaaaaact caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacac
    11401 gcctgttgcc tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa
    11461 ccgatgaaag taagctggca atgacagtca tggcggttga tgacaacccc gctaacctga
    11521 aacttatcgg cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc
    11581 atcaggcggt tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa
    11641 tgccggacat ggatggcatt cgggcctgcg aactcatcca ccaactcccg catcagcaac
    11701 aaacgccggt tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg
    11761 gcgcagggat gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt
    11821 tgttgcgcta caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg
    11881 aaattgtggt gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag
    11941 gaaaaaccga tttagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc
    12001 gcaacaaagt tgaggaacag ctggttggag aaaacccgga aggcctggtt gatttgattc
    12061 ataaactgca tggcagttgc ggctatagcg gtgtgccacg tatgaagaat ctctgccaac
    12121 tgatcgaaca acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg
    12181 aactgttgga cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga
    12241 cgtcttgtcg agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc
    12301 ctgtgtttat gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc
    12361 aataaattaa aaagcatgta ggcctgataa gcgtagcgca tcaggcaatt ttgtgtttgg
    12421 catcagtctc aaaccggctc ctgacagagc cggttttggt tttctgtctt aacgcaccat
    12481 gcacgggcgc ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc
    12541 gtcacgcgcg ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat
    12601 atcgatttct acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc
    12661 aaacggttct ttggtcaggc gctgattgcc ttcctgccaa atccagtgcg tatcaatagc
    12721 agtaatttta cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa
    12781 gtggttgtta gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg
    12841 taccgaacct tgcattgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag
    12901 ggagagcgta tggcccattt gccgccagtc ggtggcgatc atattggttg cagtcggtag
    12961 acctgtcgcg cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc
    13021 acacggatct tctgcataag ccagcgaacc tttcaggtat ttaccgattt taatcgcttc
    13081 gttcagcgac caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc
    13141 cagtgccaca atagactcgg cctcttcttc cccggccagt acaccgccct tcagtttgaa
    13201 atcgttgaag ccatattttt catatgccgc ttccgccagg cgcaccaccg catccggcgt
    13261 catcgcttct tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata
    13321 cggcagcggc gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc
    13381 gctacgctgt tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag
    13441 cagatccagc attgccgctt ctatcccggt aactacatga atagtggtac gtaggtcaaa
    13501 tgtctgcaaa ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag
    13561 aacgtttttg tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt
    13621 tttacggatt ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt
    13681 gataatcaca atattacgcg taaagaacgg tgcgtgtgca ccactcagat tcatcagcat
    13741 actgtcatga cccgccaccg ggataacctg catttcagta acaacaggcg tcgtaaattg
    13801 agaactcata tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg
    13861 tcaaaggtcc agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcca
    13921 ggcagacgtt tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc
    13981 agaccgggcg catcaggaac ggcaattttt ccgtttttaa tctccagcgg attttgggtc
    14041 aggcgacaat cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt
    14101 gccgccgcgc ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg
    14161 cagccccagg tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc
    14221 gtccagaagt gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc
    14281 atttcgcgcc agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga
    14341 aattccgcca tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca
    14401 taggtaagaa catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca
    14461 ccgttgggat caacggtaat ccgcgcatcc gggaagcgtt tcttcaatgc acgaacagtg
    14521 tcgatttctt gctcgccagg taacacgccg cccttaagtt tgaaatcttt aaagccgtag
    14581 cgatcctgtg aggcttccgc cagacgcaca acggcttcgc tgttcattgc tttctgatgc
    14641 cgcaactgat accactcatg gttgcccggc gtattttcca cataaggaag atcggttttg
    14701 gtccgatcac cgatataaaa cagataaccg aggacggtaa tagcctcgcg ttgcttgcct
    14761 ggccctaaca gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaagcg
    14821 gcttccagcg ccgccacggc gttaacgcgt aattcaaagg tccaggcacc tttaccgaag
    14881 gtatcaaaat cggcggcctg attaccttta tgcacctgct ggaccacttt attcagtcgc
    14941 gcaacttcct ggcccagaac catcggaata gcatcgacaa gcgtctgata aatcacatct
    15001 ccgcccggcg cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacaatattg
    15061 cgggtgaaat atgcgttatg tgccccacca atattaagca acatgctgtc atgcccggcc
    15121 accggaatga ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc
    15181 cgcaacaggt ttcaactcga tacgcttgat atcgcccacc agcaccaggt agctcagtac
    15241 cgcgattaag gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc
    15301 aacgatataa ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa
    15361 caggccaccg gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc
    15421 cagcgccccg atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc
    15481 aacgttgacg tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg
    15541 tgttttacgc gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc
    15601 acccagcaca ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt
    15661 cagaatcgac atcccgcgtg cctgcaccag ataaaccggg aaccaggtaa taaagaagta
    15721 agtcagggcg ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgaccctag
    15781 cagctgtttg atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg
    15841 atccatattg atcagcgcac cacccgcggc gatgtactcc agctctttct tatttacccc
    15901 cggatgttga tttggctcgt ggatgacttt caaccagata aagctgatga caatccccag
    15961 accgcccata aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat
    16021 cggcgcgaag atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc
    16081 cctttcctgc gccggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc
    16141 ttcagcaagc ccgaccagga agcgcagcgt aaacagggca acgataatgc cgaatccact
    16201 aaagatatcg acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa
    16261 gtagacgcgt tttgaaccaa aacggtccag caaccagcca ccagggatct gcccgataac
    16321 ataagcccat gagaaagcag agaacacata gcccattccc acgggatcaa ggccgatatc
    16381 tttggccatt tccgaaccgg cgatagagag cgtagcgcgg tcgccgtagt tgaaggatgt
    16441 gacgataaac aacatcacca ctatccagta acgagcattt gtgcgttttt ccacactgct
    16501 cgcagcctga cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca
    16561 ctctggactg cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt
    16621 ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc
    16681 gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg
    16741 gcattagccc tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa
    16801 gggttaaaac gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc
    16861 cagtaaaaat ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc
    16921 agcaccgcta aaacgacatt taccgctcgc tgaacatatc aggacaacag cgtgccccac
    16981 tgttcgaccc acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac
    17041 agcatttcgc caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg
    17101 ccattgcaga aattcacata actactgtcg ccgagcgcaa tcacgccata acgcagattc
    17161 ggctggaagc ccagactatc tttgattccc tgaaagagtg gcacaatgct atcaggaagg
    17221 tcgccctgcc cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag
    17281 tcgcttaatt caggatcttc aaataccgtt gctttgtggc cctgcgcggt cagaatcgct
    17341 tccgcttctt cggccactaa cagtgaattc ccgtacatgg tgccgacaaa aataccaatt
    17401 tccgccatac cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg
    17461 cactgaactc aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg
    17521 cctgcatcca ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat
    17581 gcgtcaatga caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac
    17641 cgctgcgatt ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat
    17701 gggcaagatg tcggcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc
    17761 cgtagcgcgt ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc
    17821 gataatgcgt cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt
    17881 tgtccagttc ttccaccagt ggataatcca gcaccgcttc ttccatcaac cagccgcgca
    17941 caatcgcatg gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc
    18001 cggcctcgct ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag
    18061 cagtaaaaac atgctggcct atctggtcac gcacggtttg catgaccact actttctcgt
    18121 cgcgatccag ccagctgcgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc
    18181 attcatcctg atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag
    18241 tttttccagc tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc
    18301 ataatagggt gcaacggcaa aagcgcccgg taacggctgt ttgttatcta acaaatcgtg
    18361 cattcgcggg atcagcaccc actgcaacca ctccagtggt tccatggtgt ccataaagaa
    18421 cggttgggta ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc
    18481 acgcagtaac gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa
    18541 gcaacctcaa taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt
    18601 attcacagcg ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca
    18661 tccttgacaa cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct
    18721 gctcacacca ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc
    18781 gaatcatcct gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt
    18841 catcctcttc gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag
    18901 aagtgtcatc atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc
    18961 gtctttttcg cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat
    19021 gaaagttaag aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt
    19081 tttaagtatt cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa
    19141 ccagatgggg tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt
    19201 tgaggctaaa tgcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag
    19261 attacacacc gagatgacct ctaactcttc ttccagcgtc gcgataaata gtgtgggcgg
    19321 cagttttagc cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa
    19381 gtcgtcttca ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat
    19441 atccccggca aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa
    19501 agcgcgttca accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac
    19561 ggcatcttcg gtagttgaaa taatgcacgg tgaaggaaca ccgtacagtt cctcacttaa
    19621 cggccagctt ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag
    19681 ggcttgtgcg gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc
    19741 gtttagttta tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact
    19801 tgcgggcctg actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact
    19861 gcaaggcgtt ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc
    19921 ttttcacggt acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt
    19981 gccgcaggtc gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc
    20041 gaagagtttt aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga
    20101 ggtgcgccag acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc
    20161 gttatatcgt cttgatgaac tggaaggcca gccgataggt cattttaatg gcacttgcat
    20221 tgatgaccag gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc
    20281 cacctgtggt gaaaaagtag tggaagagac acttgtcagc cacctgctga aatcaaactg
    20341 cctgatcacc catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat
    20401 tgacagagaa aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga
    20461 acagtgcgtg gaacgcatct ttaatgacct gttacgcttc tgccagccag aaaaattgag
    20521 cgtttacgca cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag
    20581 cgattttgtc ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt
    20641 gctggattca cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga
    20701 atgacgatat tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc
    20761 catggatatg ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct
    20821 ctatcaactg tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa
    20881 cagcaaagaa ttgctgtctc gttttgaaaa tttcgatatt aacgtcttgc gccgtgaacg
    20941 cggcgtaaag ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat
    21001 tcgcgctttg caggccaact tgttcgcggt cctgcgtgac attctcttcg tttacgggca
    21061 aatccataac accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa
    21121 cctggtcttt tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat
    21181 ggtggtctgc tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt
    21241 cggaaaccag ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc cgggagcgat
    21301 ggaagcgccg atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg
    21361 ttttattggt atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa
    21421 cgaattgatc atcatgccag atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca
    21481 cggtatcatt atcttccctg gcggtgtggg tacggcagaa gagttgctct atttgctggg
    21541 aattttaatg aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa
    21601 agagagcgcc gactacttcc gcgtactgga cgagtttgtc gtgcatacgc tgggtgaaaa
    21661 cgcgcgccgc cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa
    21721 aaaatcgatg ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa
    21781 ctggtcaatg cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat
    21841 ggctaatctg aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg
    21901 cgcgttctcc ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga
    21961 gtttggtcct tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct
    22021 acagggtttt gttgcccagc atcgtatgaa gctgccaggc tcagcctaca tcccttgcta
    22081 cgaaatctgc acgtaatctc cgctcccccg gtgacgtttg ttgccgggtt ttctcgtttt
    22141 tggtcactta ctcatcaact catttcattt gttatatgaa tgtttcttac caccctcacg
    22201 cgacaaatat catcacagtt aatatgtcat acaatttatg ttgcaacgca aacgtttccc
    22261 tattttcata aaccgtattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa
    22321 agtattgatt ttttcagttc aacctacata tattgcgcgc cccggaagaa gtcagatgtc
    22381 gtttaatggg caaatattgc ccttaaattc tcttttactt ttgatttaca gagtaaagcg
    22441 ttgggataat ctatcttcca agtagattat tgtatttggg atcaagatca ctgatagata
    22501 cataacttgt gtgtatcttt ccgccctcaa attattacgg cggtaaatga ttaagccatc
    22561 gccgatagac agatttcatt tttacggtca ggcaccttcc cgggctgaac tggctaaaag
    22621 ctgaattatt tgcattcctc caggagaaat agatggaaac gactcaaacc agcacgattg
    22681 cgtcgaaaga ctctcgtagt gcctggcgca agacagacac catgtggatg ctgggccttt
    22741 acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt gttggcggta
    22801 tgatcccgct gatcatcatg gctatccttg cgttcccgat gacgtttttt gctcaccgcg
    22861 gcctgactcg cttcgtactg tctggtaaaa acccgggcga agacatcacc gaggttgtag
    22921 aagaacactt tggtattggc gcaggtaaac tgattacctt gctctatttc ttcgctatct
    22981 acccgatcct gctggtttat agcgtagcaa tcaccaatac agttgaaagc ttcatgtctc
    23041 accagctggg tatgacacca ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta
    23101 tgatgaccat cgttcgcttc ggtgagcaga tgatcgttaa agcgatgagt attctggtat
    23161 tcccgtttgt tggcgtactg atgctgctgg ctctgtacct gatcccgcag tggaacggcg
    23221 cagcactgga aacgctgtct ctggacactg catctgcaac cgggaacggt ctgtggatga
    23281 ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa ccactctccg atcatctctt
    23341 ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa tgctcgaaga
    23401 tccttgcatt cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc ttcagctgcg
    23461 tactgagcct gactccggca gacctggctg cggctaaaga gcagaacatc tcgattctgt
    23521 cttacctggc taaccacttt aacgcaccgg ttatcgcgtg gatggctccg attatcgcga
    23581 ttatcgctat caccaaatcc ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg
    23641 gtatggtgat taaatctctg cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc
    23701 gtatcactgc gctgttcatg ctggtaacga cctggattgt tgccaccctg aacccgagca
    23761 tcctgggtat gattgaaacc ctgggcggcc caatcatcgc gatgatcctg ttcctgatgc
    23821 cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg
    23881 tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat cttctactct ctgttcagct
    23941 aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta
    24001 tgattagcgt attcgatatt ttcaaaatcg gcattggccc ttccagttct cataccgttg
    24061 gaccaatgaa agcgggtaaa caatttaccg acgatctgat tgcccataat ctgcttaaag
    24121 acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc tctgaccggt aaaggccacc
    24181 acactgatat cgccattatt atgggcctgg cgggtaacct gccggatacc gtggatatcg
    24241 attccatccc tggttttatt caggatgtga atactcatgg tcgcctgatg ctggcaaacg
    24301 gtcagcatga agtggagttc ccggttgatc agtgcatgaa cttccatgca gacaaccttt
    24361 ctctgcatga aaacggtatg cgcattaccg cgctggcggg cgataaagtc gtttacagcc
    24421 agacttacta ctctattggc ggtggcttta tcgttgatga agagcatttt ggccagcaga
    24481 atagcgcacc ggttgaagtt ccttatccgt acagttcagc agccgatctg caaaaacatt
    24541 gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa aaatgagctg gcgctgcaca
    24601 gcaaagaaga gctggaacag cacctggcga acgtatggga agtcatgcgt ggcggtattg
    24661 agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg
    24721 cggcactacg ccggatgctg gtcagccagg ataaaaccac cactgacccg atggcggttg
    24781 ttgactggat caacatgttt gcactggcag tgaacgaaga gaacgctgct ggcggacgcg
    24841 tggtgactgc gccgactaac ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg
    24901 acaagtttat ccgcgaagtg aacgctaact cactggctcg ttacctgctg gtagccagtg
    24961 ccattggttc tctttataag atgaacgcgt caatttctgg tgctgaagtc ggctgccagg
    25021 gtgaagttgg tgtggcgtgc tcaatggcgg cggctggtct ggcagagctg ttaggtgcaa
    25081 gcccggcgca ggtgtgcatc gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt
    25141 gtgacccggt cgccggacag gtacaggtgc catgcatcga gcgtaacgcc attgcggcag
    25201 taaaagcggt gaacgccgca cgtatggcgc tgcgccgtac cagcgagccg cgcgtctgcc
    25261 tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg
    25321 aaacctctcg cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg ctctccagag
    25381 gcctcgtttt gcgaggcctc ttcccgattt ctcatccagc cgtagcctgt tccggcatcg
    25441 aatgttaccc ttatcgcctg atctttaagg gggttatcgt ggctgttcat ttgcttattg
    25501 tcgatgcact gaatcttatt cgtcgcattc atgccgttca ggggtcgccc tgtgtcgaaa
    25561 cctgccagca tgcgctcgat cagctcatta tgcacagcca gccaacccac gcggtcgccg
    25621 tttttgatga tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca gattacaaag
    25681 cgggtcgacc gccaatgccg gaagagttgc acgacgagat gcctgcatta cgcgccgcct
    25741 ttgagcaacg cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat gacttagccg
    25801 ccacgctggc ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt tcgacagata
    25861 aaggctactg tcagttactt tcaccgacat tacgtattcg tgattacttc cagaaacgtt
    25921 ggctggatgc gccatttatc gataaagaat ttggcgttca accgcagcag ttgcccgatt
    25981 actggggact tgcggggatc agcagttcaa aggtaccggg tgttgcggga atcggaccaa
    26041 aaagcgccac gcagctgctg gtcgagtttc agagtctgga agggatatat gagaatctgg
    26101 atgcggttgc cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt
    26161 gccgcgatat tgcccgctta caaaccgatt tgcatatcga cggcaattta cagcaattgc
    26221 ggttggtacg gtaacggcga gccggatacg ccgcaaacgt cgtatccggc attatcacat
    26281 cagcgcattt accaggcggt atggtaaagc tctacaatat cctcaagcgt tgcttcacgc
    26341 gggttgccac cggtacaaac atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc
    26401 ttgcgtacac caacatcacg caaatgtggc ggaataccga catcacggtt gagagcaaac
    26461 accgcttcaa cagcggcatt acgcgcctct tccaggctca taccttccac tttcacgccc
    26521 ataacgcgcg cgatatcgcg gtacttctca ccggtaaagt cagcgttata acgcatgaca
    26581 tgcggtaaca ggatggcgtt cgcaacaccg tgtggagtgt tataaaacgc gcccagtgga
    26641 tgcgccatac catgcaccaa ccctaaccca acattcgaga agcccatacc cgcaacatac
    26701 tgcccgagcg ccatttcttc tccggcatcc ttatcaccag caaccgatcc tcgcagcgcc
    26761 ccagcaatga tttcaatcgc tttaatgtgc agtgcatcgg ttagcgccca cgcgccacgg
    26821 gtaatatacc cctcaatagc atgagtgagc gcatcgacac ccgtcgcagc tttcagcgct
    26881 ggaggcatac catccatcat gtcagcgtca ataaacgcca cctgcgggat atcatgcgga
    26941 tcaacgcaaa caaacttgcg ccgtttctct tcgtcagtga tcacgtagtt aatggtcact
    27001 tctgccgcag tacctgctgt ggtaggaatt gccagaatcg gtacactggg tttattggtc
    27061 ggggaaagcc cttccaggct acgcacatcg gcaaactccg ggttgttgct gataatgcca
    27121 atcgctttac aagtatcctg tggagaacca ccaccaatag cgatcaggta atccgcgccg
    27181 ctattctgga atacaccgag cccttctttg acgacagtaa ttgttgggtt gggcactacg
    27241 ccgtcgtaaa tcgcccatgc cagccctgca gcatccatct tatcggtcac tttcgccacc
    27301 acgccgcatt gcaccagcgt tttatcggtg acgatcagcg ccttctgata accacggcgt
    27361 ttcacctcat cggttaaagc cccaacagca ccccgaccaa accatgccgt ttcgttcaga
    27421 atcattctgt tagccatcat ccttctcctt gttgctttac gaaattactc ttcaattcgt
    27481 aacccatagg ttttgaattt ctccagcact acggcaatct cttcatcgct cagcactggc
    27541 accgggtccg taatcgccag ggtcgtcagg taaagttgcg ccagcacttc aacttcatgc
    27601 gccagccata acgctttttc cagattcacc tcacaagcga taagcccatg atgttgtaac
    27661 aaagttgcct tacgattttt gagagccagc gcaacatgtt cagaaagttc gcgtgttcca
    27721 aaggtcgcat aaggcgcgca aggaatagaa ttaccgccag ccgccgcaat catgtagtga
    27781 atagcgggga tcgatcggtt aagaatggaa actgccgtgc aatgaacggc atgattgtga
    27841 acaaccgcgt tggcatccgg tctgctttga taggctgcca tatggaaacg ccattcgctt
    27901 gaggggagct ttccttcctc atgtttaccg ttgccatcaa taaagacaat atgcgactcc
    27961 gtcagttttt catatggaat gcctgtaggc gtaatcagca tcccatcctg ataacgtaca
    28021 ctgacgttcc ccgctgtccc ctggttcagt cccaggcggg tcatttccag gcaagtgtca
    28081 ataatctgac gagcaagttt atttcgttcc attagctacc tctctctgat tcaaaacagg
    28141 gcaataatgt tgttcctttc acactattga attagccgtt taattaccca ccatcttctt
    28201 cctgattaac aagaaagaaa ttcacaagct tatattttgt gacctggttc aactaatcac
    28261 agtaaataac tgcaa
//
LOCUS       DAZFZC010000069        27912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFZC010000069 DAZFZC010000000
VERSION     DAZFZC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.069"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..1001
                     /locus_tag="ACHKXP_003241"
     CDS             33..1001
                     /locus_tag="ACHKXP_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HFO7648193.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEVKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            1026..1157
                     /locus_tag="ACHKXP_003242"
     CDS             1026..1157
                     /locus_tag="ACHKXP_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO7648194.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            1142..1294
                     /gene="kil"
                     /locus_tag="ACHKXP_003243"
     CDS             1142..1294
                     /gene="kil"
                     /locus_tag="ACHKXP_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO7648195.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            1549..2256
                     /locus_tag="ACHKXP_003244"
     CDS             1549..2256
                     /locus_tag="ACHKXP_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HFO7648196.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYAATETDKKKLIERYQHDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            2257..2772
                     /gene="ssb"
                     /locus_tag="ACHKXP_003245"
     CDS             2257..2772
                     /gene="ssb"
                     /locus_tag="ACHKXP_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7648197.1"
                     /translation="MASRGVNKVIIIGRLGHDPEIRYSPSGTAFANLTVATSEQWRDK
                     QTGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYLEGKLRTRKWQDQSGQDRFTTEV
                     IVGVGGTMQMLGGKQGGNEQSSPQRNNGQQQRQQPQQQGNHSEPPMDFDDDIPFAPVI
                     LPFPRHAIHAI"
     gene            2786..3082
                     /locus_tag="ACHKXP_003246"
     CDS             2786..3082
                     /locus_tag="ACHKXP_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO7648198.1"
                     /translation="MPAPLYGAEDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEYIDPRLRNYRSRYGAISND"
     gene            3093..3257
                     /locus_tag="ACHKXP_003247"
     CDS             3093..3257
                     /locus_tag="ACHKXP_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO7648199.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
     gene            3254..3769
                     /locus_tag="ACHKXP_003248"
     CDS             3254..3769
                     /locus_tag="ACHKXP_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7648200.1"
                     /translation="MKQMSLIEMDGFLKGKCIPRDLMVNETNAEYLVRKFAEAEAKIS
                     ALSEDQQRAIESIKQADAAVKLAHEKFSALAAENELARKAVQAFCDVVGDNTEVIAEV
                     VGRDGVLVILEAMKATGNMPATDAFLAEVRAQGVEMAMEHMQSSGSLTFGDCYISLNE
                     FAAQLRKGGNQ"
     gene            3766..4347
                     /locus_tag="ACHKXP_003249"
     CDS             3766..4347
                     /locus_tag="ACHKXP_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7648201.1"
                     /translation="MSKINYQALREKAEKATCGVWSLEYGEERLDAGDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAASPVTVLALLDERERNQQYIKCRDQE
                     NEDIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            4349..5104
                     /locus_tag="ACHKXP_003250"
     CDS             4349..5104
                     /locus_tag="ACHKXP_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648202.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEFCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHMNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAV"
     gene            5115..5561
                     /locus_tag="ACHKXP_003251"
                     /pseudo
     CDS             5115..5561
                     /locus_tag="ACHKXP_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5581..5790
                     /locus_tag="ACHKXP_003252"
     CDS             5581..5790
                     /locus_tag="ACHKXP_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648203.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRVNK"
     gene            5787..6422
                     /locus_tag="ACHKXP_003253"
                     /pseudo
     CDS             5787..6422
                     /locus_tag="ACHKXP_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            6708..7004
                     /locus_tag="ACHKXP_003254"
     CDS             6708..7004
                     /locus_tag="ACHKXP_003254"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7648204.1"
                     /translation="MLDRIDTLENCDDDRIEAVKAVLRRLAGNYPVIPDGWISCSERM
                     PDKLIPVMVMYEDGEMWSAMWNGNRWDDGTEYPDPHSVTHWREMPAAPKQEVKS"
     gene            7001..7213
                     /locus_tag="ACHKXP_003255"
     CDS             7001..7213
                     /locus_tag="ACHKXP_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648205.1"
                     /translation="MSKHIIKYDYREGVKLAKHEIETWCGHAPQFSDWLFQDAQHALL
                     SIEQGTLLVPCKNCLAAIIKTAQEVK"
     gene            7213..7491
                     /locus_tag="ACHKXP_003256"
     CDS             7213..7491
                     /locus_tag="ACHKXP_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO7648206.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGSVIVVTTQSGMTIQIHKPKEEK"
     gene            7646..7813
                     /locus_tag="ACHKXP_003257"
     CDS             7646..7813
                     /locus_tag="ACHKXP_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648207.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAENINDCYDHWMIWAQIAHANVTNI
                     RIEELKEHQTA"
     gene            7844..8035
                     /locus_tag="ACHKXP_003258"
     CDS             7844..8035
                     /locus_tag="ACHKXP_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7648208.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(8016..9194)
                     /locus_tag="ACHKXP_003259"
     CDS             complement(8016..9194)
                     /locus_tag="ACHKXP_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7648209.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            9376..10800
                     /gene="sbcB"
                     /locus_tag="ACHKXP_003260"
     CDS             9376..10800
                     /gene="sbcB"
                     /locus_tag="ACHKXP_003260"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO7648210.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEATFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(10843..11070)
                     /gene="tsuB"
                     /locus_tag="ACHKXP_003261"
     CDS             complement(10843..11070)
                     /gene="tsuB"
                     /locus_tag="ACHKXP_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO7648211.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(11084..12142)
                     /gene="tsuA"
                     /locus_tag="ACHKXP_003262"
     CDS             complement(11084..12142)
                     /gene="tsuA"
                     /locus_tag="ACHKXP_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO7648212.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(12321..13679)
                     /gene="plaP"
                     /locus_tag="ACHKXP_003263"
     CDS             complement(12321..13679)
                     /gene="plaP"
                     /locus_tag="ACHKXP_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO7648213.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(13669..13731)
                     /gene="yoeI"
                     /locus_tag="ACHKXP_003264"
     CDS             complement(13669..13731)
                     /gene="yoeI"
                     /locus_tag="ACHKXP_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO7648214.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(13946..14875)
                     /gene="yeeY"
                     /locus_tag="ACHKXP_003265"
     CDS             complement(13946..14875)
                     /gene="yeeY"
                     /locus_tag="ACHKXP_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7648215.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(14921..15745)
                     /gene="yeeZ"
                     /locus_tag="ACHKXP_003266"
     CDS             complement(14921..15745)
                     /gene="yeeZ"
                     /locus_tag="ACHKXP_003266"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7648216.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDTDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEEFEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(15828..16082)
                     /gene="yoeB"
                     /locus_tag="ACHKXP_003267"
     CDS             complement(15828..16082)
                     /gene="yoeB"
                     /locus_tag="ACHKXP_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO7648217.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(16079..16330)
                     /gene="yefM"
                     /locus_tag="ACHKXP_003268"
     CDS             complement(16079..16330)
                     /gene="yefM"
                     /locus_tag="ACHKXP_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO7648218.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            16614..16664
                     /gene="hisL"
                     /locus_tag="ACHKXP_003269"
     CDS             16614..16664
                     /gene="hisL"
                     /locus_tag="ACHKXP_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO7648219.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    16640..16763
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            16810..17709
                     /gene="hisG"
                     /locus_tag="ACHKXP_003270"
     CDS             16810..17709
                     /gene="hisG"
                     /locus_tag="ACHKXP_003270"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO7648220.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            17715..19019
                     /gene="hisD"
                     /locus_tag="ACHKXP_003271"
     CDS             17715..19019
                     /gene="hisD"
                     /locus_tag="ACHKXP_003271"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO7648221.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            19016..20086
                     /gene="hisC"
                     /locus_tag="ACHKXP_003272"
     CDS             19016..20086
                     /gene="hisC"
                     /locus_tag="ACHKXP_003272"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO7648222.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            20086..21153
                     /gene="hisB"
                     /locus_tag="ACHKXP_003273"
     CDS             20086..21153
                     /gene="hisB"
                     /locus_tag="ACHKXP_003273"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO7648223.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFLQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            21153..21743
                     /gene="hisH"
                     /locus_tag="ACHKXP_003274"
     CDS             21153..21743
                     /gene="hisH"
                     /locus_tag="ACHKXP_003274"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO7648224.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            21743..22480
                     /gene="hisA"
                     /locus_tag="ACHKXP_003275"
     CDS             21743..22480
                     /gene="hisA"
                     /locus_tag="ACHKXP_003275"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO7648225.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            22462..23238
                     /gene="hisF"
                     /locus_tag="ACHKXP_003276"
     CDS             22462..23238
                     /gene="hisF"
                     /locus_tag="ACHKXP_003276"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO7648226.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            23232..23843
                     /gene="hisIE"
                     /locus_tag="ACHKXP_003277"
     CDS             23232..23843
                     /gene="hisIE"
                     /locus_tag="ACHKXP_003277"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO7648227.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPKALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(23940..24917)
                     /gene="wzzB"
                     /locus_tag="ACHKXP_003278"
     CDS             complement(23940..24917)
                     /gene="wzzB"
                     /locus_tag="ACHKXP_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO7648228.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQTSLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQPLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPVRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(25064..26230)
                     /gene="ugd"
                     /locus_tag="ACHKXP_003279"
     CDS             complement(25064..26230)
                     /gene="ugd"
                     /locus_tag="ACHKXP_003279"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO7648229.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKECLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(26479..27885)
                     /gene="gndA"
                     /locus_tag="ACHKXP_003280"
     CDS             complement(26479..27885)
                     /gene="gndA"
                     /locus_tag="ACHKXP_003280"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO7648230.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKTEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         7495 a         6821 c         7090 g         6506 t
ORIGIN      
        1 aatgtgaggg cgattttttt gacggaggat atatgagtga agtaacagat ttagttgtta
       61 ttgaaaaagc aaatgcaatg accgtatttc agtctgccga ccagattgaa gaaatccttc
      121 aaaaggttga acgtgaagtt atgtcctttg tgcctgatat cacaacggca aagggcagaa
      181 aggagatcgc ttctctggcg tataaagttg cgcagacgaa aacatatctc gatggtcttg
      241 gcaaagacct tgttgctgaa ctgaaggaaa ttccaaagct aattgatgct aaccgcaaga
      301 cagtgcgtga tcgccttgat gaactgaaag ccaaggcgcg ccagcctctt actgattatg
      361 aggaggaaca ggcacggatt aaagccgaag aagaagctaa ggcagcagct gaagctctcg
      421 caaagcaaat tgagtctgac catgaaatag cgattttgat ggatcgcgaa tttgaccgcc
      481 aaagagaaga ggcaagactc aaagcggagc aggaaaagcg agagcatgaa gaacgcttaa
      541 aaagagaagc tgaagagaaa gccagagctg aagccgaagc aaaggcaaaa gccgaaattg
      601 aagcagcagc aaggcgagaa gcagaagcta aggccgcagc ggaacgtgca gagcgtgaac
      661 gcattgaagc cgagcaacga gcacagcgcg aagcaaaaga ggcagcagaa cgagctgaaa
      721 gagaaaagca ggcggcaatt gaagcagaac gccgaaaagc acaggaggag gctgaacgaa
      781 tccggcgcga ggctgaagta aaagagcaag ccagaatagc agaagaaaaa agaatcaagg
      841 acgaagaaga gcgtagagca aaggataaag ctcaccggaa agaagtaaat aacaaaatac
      901 ttgctgacct tatcaaggtt ggcgcatcag aagatgttgc taaaaatatc ataacagcca
      961 tcgtaaaagg cgaagtattc gcaacaaaaa taacctacta ataaaaccaa cataaggaac
     1021 cacccatgat ttacgcaatc gcgggaggcg ctcgcatggg tgccttccaa ctaaatgaat
     1081 ctttacttga acgaatcacc cgtaaattac gtgacggatg gaaaagagtt gaggtcttat
     1141 tatgcgcaat gaaatagcca ttaatcacca gatgcttcgt gcggcacaaa acaaagcagt
     1201 aatagccaga tttattggtg attcaaaaat gtggcttgaa gcaaataaag cgatgaaatc
     1261 agccatcaac cttccgtggt atcgcaggaa atgagtttta cagataactg gtcagacgaa
     1321 gaattcattc gtcagatgaa agatttaatc ggtaacgaag gagatattca tgtcacttgc
     1381 aaccacagtg aaggagagca agttacagag acgcatgtac acgcagaaag ctctctggta
     1441 tcgccataat ggcgaccgcg aaggaatgcg ggtatgcctt aatttgtccc gagtcgaagt
     1501 attaaatcag cgttatttcc ttgggccgtg tccattctga ggtgaattat ggatttgaat
     1561 aaattcgatg agccattcag ccccgaagat atcgaatggc gaatacagca aagcggtaaa
     1621 acacgcgatg gcaaggtgtg ggctatggtg ctggcttatg tcacgaaccg ggcaatcatg
     1681 aaacgcctgg acgatgtttg cggcaaagca ggatggcgca atgaataccg cgatattccc
     1741 aacaacggcg gagttgaatg cggcatatca atcaagattg attccgaatg ggtaaccaaa
     1801 tgggatgctg ctgaaaacac gcaggtagaa gccgtcaaag gtggtcgttc cggtgcaatg
     1861 aagcgtgctg ccgttcagtg gggaatcggt cggtatctgt ataaccttga ggaaggtttc
     1921 gcacaaacat ctctcgacaa aaagcaggga tggcacaggg caaaactcaa ggatggaaca
     1981 ggattttact ggctccctcc atcgctgccg ggctgggcaa tcccagcatc agataacaaa
     2041 ccatcaccag aaaataccaa ccagaaatct ccatcggttg actgcgaaca aatcctgaaa
     2101 gacttcagcg attatgcagc gacagaaact gacaagaaaa aactcatcga gcgttatcag
     2161 catgactggc aattaatggc tggaaacgag gaggcgcagg ctaaatgcgt tcaggtaatg
     2221 aacatcagag ttaacgagct aaaacaggcg gcataaatgg caagcagagg cgtaaataag
     2281 gtgatcatta ttggtcgcct tgggcatgat ccagaaatca gatattcacc atcaggaacg
     2341 gcatttgcaa accttacagt tgctacgtca gaacaatggc gtgataagca aactggagag
     2401 caaaaggagc agacggagtg gcaccgcgtg gtaatgagcg ggaaactggc agaaattgcc
     2461 agcgaatatc tgcgaaaagg ctctgaggtt tatcttgaag gaaaattgcg gacaagaaaa
     2521 tggcaggatc aaagcggaca ggatcggttc actaccgaag tcatcgtggg cgttggtgga
     2581 accatgcaaa tgcttggtgg caagcaagga ggcaatgaac agtcttcacc tcagcgaaat
     2641 aatggtcagc aacaaagaca gcaacctcag cagcagggaa atcacagcga accacctatg
     2701 gattttgacg acgatatacc ctttgcacca gtaattctcc ccttccctcg tcacgctatt
     2761 cacgcaattt aatcaggaga aaatcatgcc agcgcctctg tatggtgcgg aggacccgcg
     2821 ccgctgttcc ggcaattccg tatcggaggt gctggataaa ttcagaaaaa actacgatcg
     2881 aataatgtcg ctaccgcagg aaacgaaaga ggaaaaggaa tttcgccact gtatatggct
     2941 tgcagagaaa gaagaacgcg agcgaattta ccagacatca atccgaccat tccgcaaagc
     3001 cacatatacc cacttccctg aatatatcga cccgcgcctg cgtaattacc gctcacgcta
     3061 tggcgctatc agtaatgact gaggaattta ccatgagagg acttgcatac aatcccggca
     3121 ttcttccggc agaaatgatt attcgccaac gcgtaaagcc aatgccatcg agagaggaat
     3181 tgcttaagag aaattctttt ccgtcagtaa atcaaaacaa atatctgaat gcgatgtggc
     3241 ggagtgggaa gaaatgaaac aaatgtcact aattgagatg gatggttttc tgaaaggtaa
     3301 atgcatccca cgagatttaa tggttaacga aacaaacgct gaatatctgg tgcgtaaatt
     3361 tgctgaagcg gaggccaaga tttcggctct gtccgaagac cagcagagag cgattgagtc
     3421 aattaagcag gctgatgcag ctgttaagtt ggcacacgag aagttttcgg cgcttgcggc
     3481 ggagaatgag ctggctcgta aggcagttca ggcattctgc gatgttgttg gcgacaacac
     3541 cgaggttatc gctgaggtgg ttgggcgaga tggcgttctt gttattttgg aggccatgaa
     3601 ggcaacagga aatatgccag ccaccgatgc tttcctggct gaagtacggg cgcagggcgt
     3661 ggagatggct atggagcata tgcagtcgag cggttcgtta acatttggag attgctacat
     3721 atcacttaac gagttcgccg cccagcttcg taaaggaggc aaccagtgag caagattaac
     3781 tatcaggcac tgcgtgaaaa ggcagagaaa gcaacgtgtg gtgtgtggtc gctcgaatat
     3841 ggagaggaga gacttgatgc tggtgatgca ctaattcatc gtgaagttgt tggatatctt
     3901 cccatttgca gaattgaagg agcgcatcct gaaagcggtt tcgatgaaga tttccaaatg
     3961 gaacagcagg ccaatgctga attcatcgcc gcagccagtc cagttaccgt gctggcattg
     4021 ttggatgaac gggaaagaaa ccagcaatac atcaaatgcc gcgaccagga gaacgaggat
     4081 attgcgctaa cggtagggaa gctgagagtt gagttggaag cagcaaaatc aaaactcaac
     4141 gagcagcgtg aatattacga gggagtaatc gcggatggaa gtaagcgcat tgctgaactg
     4201 gaagcgcggg aaatactgct cccggaacgt agcagcatgc ttcatcgaac agattttcac
     4261 gatgattacc aaacggtaat ggcatacaaa gtttctgaag tcatcgatgc aatccgcgct
     4321 actggcattc gcatcaaagg agagtgagat gattcactac cacggtgggc ctattactcc
     4381 tgatacgtgc gcaatgagag catggaaagg gcgacatgcg tttatcagtt ttgcgcattc
     4441 aggccagatc aatctcgcgg ctgaattctg tcagtcgttc gcgctggaca acggtgcatt
     4501 caccgcctgg aaagcagctg gcaaaaacaa aatcgactgg agcgattact acgagtttgt
     4561 tgctcgctgg aagaatcacc caggattcga ttttgccatt atcccggatg ttattgatgg
     4621 cggagaggag gaaaatgatg cgcttctgaa tgagtggcct cacggaaaac tagctggcgt
     4681 tccagtgtgg cacatgaatg aaagtgacga gcgatttatt catttgtgca atgagtttcc
     4741 gcgagtggct atcggtagtt gtggcgacta tgacgtaaag cgcccaactc ttgcggtagc
     4801 cagaatgaaa gacctgattc gtcacattgt tgatgggcat ggtcagccgg ttacgaaact
     4861 acatggattg cgcatgttaa atccgctgat attcacaaaa ttacccttag ccagcgcaga
     4921 tagtacgaac gtcgctcgaa acatcggtat tgataaagcc tggtctgggg cttatgcacc
     4981 tgcaagtaaa gagacacgcg cagcattaat ggtagaacgg attgaggcac acaatagccc
     5041 tggttctctt gcgtattgtg aacaacgcga ccgctttgaa atgcaattgc aactagcagt
     5101 ttaaggacta acaaatgacc actattacca ataagaaaca gtatcccagc gagcaatatc
     5161 ttaatgagct gatcaccaac atagagtttg ccgcaagggc accagttgaa gtcgtgagag
     5221 caatggcagc agagctacag aagcggcgcg aagctgatag tgcagaacca gttgcttata
     5281 ttttcaaaca tccggccggg aaattattct gggctttaac ggatgaaagc aataaagagc
     5341 aagcggacgt tattcctgtt tatgctgccg cgcctgcgtc ggttgtgccg gataatgcat
     5401 cagagcctct tgcttatgct tacaaagagc ttacgcctga gattatgcgc aaccatttag
     5461 ctgtattcga gcgatatgga atagccccaa acgatagcac taccacaatt caggcactgc
     5521 gaatcgcgct ggatggtata gagcggagcg acgccatgct tcagggtaaa ggagggtgat
     5581 atggctattg ccgcaagtta cacaatgcat ctctattgtg actgccgtca gtgcacggaa
     5641 ggtgtatatc cagtgccaga cttcggtgag tatataggta cgtcatgggc tggctgtgca
     5701 aaagaggcgc gcaaggatgg ctggcgaata agcaaagaca aaacgcgtgc ttttgcgccc
     5761 gggcataaag ttttgagggt taacaaatga ccactataac caaagagcga ctactgacaa
     5821 tcaagcagtg gcgcgaaaca tacggacctg gtagcaacgt tgtactgcca gcagaagaag
     5881 cggaagaact ggcacggatt gcgctggcat cgctggaagc agagccgata ggtttccgtt
     5941 gcaggcgcaa tgataacctt ggtgattgga gttacgtata tcatcgagag ccagatgatt
     6001 ttgagcgcaa acatttagtg atagagggca tttacgccgc ccctccagca ccggtagtgc
     6061 ctgaagaagc aactccggaa aacgtagaaa tgctctctgg ctatgtttca acgtacaaat
     6121 taaccgatag cgagcgcgat attgctgccg aaatatggaa cgcctgccgc gccgccatgc
     6181 ttcagtccgg aaactttcgg gaaaacaaga attcgtcaac caataatttt cgggaaatcg
     6241 cggaaacgtc aaccaactat ccggcaattc ctagtgaggt gttgtccgca atcctgaagg
     6301 ttgccaggat tcgtgccgat ttcgatgatt ttgacggtga caggcgaggt atcggtgatt
     6361 gtctggatga ggctgagcaa gagcttatcg ttaccattaa caaatatgcc agtcagttgg
     6421 cagcagaacc gatagcggct aatgacgttc gagagcagca gacagccgtt ccgccagttc
     6481 cagaaataca ggctgatgtc gcgcaagcaa ttgaaaatct caagcagaag ttagtggaat
     6541 gcaatcgcta taactactgt gcagatgcag ttaaaggcgt agaggatgcc tgccgtgctg
     6601 ttagctatag ccaagccgac aatcagccag catctggcaa ccaggctgcc gaatccaatc
     6661 gcgataatga gtggactggc aatcctgata ttgataacgc catcatcatg ctcgaccgca
     6721 tagatacgct ggaaaattgc gatgatgacc gtattgaggc tgttaaggct gttttgcgta
     6781 gactggctgg caactatccg gtaattccgg atggttggat aagctgtagt gagcgaatgc
     6841 cggataagtt aattccggta atggtcatgt atgaagacgg tgagatgtgg tctgcaatgt
     6901 ggaatggcaa tcgctgggat gatggcaccg aatatccgga tccgcactca gttacgcact
     6961 ggcgtgaaat gccagcagcg ccgaaacagg aggtgaagtc gtgagcaagc acatcatcaa
     7021 atatgactat cgagagggag ttaaacttgc aaagcatgaa atagaaacgt ggtgcggaca
     7081 tgcgccacaa ttttcagact ggttgtttca ggatgctcag cacgcgctgt tgagcattga
     7141 gcagggaacg ctgcttgttc cttgcaagaa ttgtctggca gcaatcatca aaacggcgca
     7201 ggaggtgaag tgatggactc cttcgcgaaa tatacaatta ttgactggat agccttcctt
     7261 caggttttgc tcatctggtt ttatatggct tacaggagtg gacagtggat tgtcagtgta
     7321 gcctgtagca agggatggcg ttggtggaac cgaaagaata aaaaagcact ggcattggat
     7381 tcgttttacg aagcattcaa tcttaacagt cttcagcctg gttctgtcat tgtagtcacc
     7441 actcaaagcg gcatgaccat tcagattcat aaaccaaaag aggaaaaatg atgtggccta
     7501 tatgtgttaa ttgcggacgg atgtgcctat ctggatggtg ccgaaagtgc gacaaatgca
     7561 cgaagaaaag acaataacat tcctcgcact cgcggggatt tcttttatct gaactcgcta
     7621 cggcggtttt ttatggagat gataaatgca cttccgagtc acaggagaat ggaatggaga
     7681 gccattcgac agagttatcg aagcagagaa catcaacgac tgctatgacc actggatgat
     7741 atgggcacag atagcacatg caaacgtaac caatattcga attgaagaac tgaaagaaca
     7801 ccaaaccgcc tgatggcggt ttctttttgc ctggagaatt aagatgaccg ataccagcct
     7861 gattcctgag aaagaagtga tgaacaagct cggtgtttca tcacgtcaga caatctggaa
     7921 ctataccaaa cggcatggat ttccgaagcc agtcagaacc caccccaaat cataccttcg
     7981 tgaagctgtt gaagggtgga ttcttaacgg tggcgttaat cagaaatgct cctgatgtgc
     8041 cagaacaatt tatcagcgtg aagctcatac gctttgcgct gagcctctat ccaatcgtgt
     8101 ttattgtata cggacattat cccgccaagt tcatgaccga gcattcgctc agtaacatga
     8161 ggctcgacat tcatctcgga cagacgtgta acaagcgttc ttctgaaatc atgtgttcgc
     8221 cagtaaccta gatccatccc tccccttatt ctatttacga atcggttggc tgcagcgatg
     8281 ctgatcggtt tgtttatgtc ttctccaggg aaaagaacat cattgtacgt tgtcatggcc
     8341 ttttcgagaa aaggcttaat ttgttcgaag attggacggc ggataacatt acccatcttg
     8401 ctatgctctt tcggtacggt ccatacgttg tccagcaggt caaattctgt ctttgttgcc
     8461 agcctaagct ctgagagcct cgccccccac agcataagca tctgatgaag gagcttattt
     8521 gacgtagacg cacggcttct ttcaatagca agccaaatct tagccagttc gtgatacgac
     8581 agtacccgat cccctacctc agcgcgggag ccgaagtccc ttggctggat gctcataatt
     8641 gcgcaactat ctatcaactg acgccgcatg caccaactaa ttgctgatct tagttgactt
     8701 agcacctgcc ttgctcggcg tggattatct ttttcttctt cggtaagcag gtcaacccat
     8761 tgcttaaccg tgatagaaga tgccggacga ttagggaagg cgtcatgcat gcgcttcata
     8821 accgttgatc ggtaaagtgc ctgggtcttt tctctgagag ttgtagagac gtagttgtcg
     8881 aaccagtagt cgagacactg ggcgaccgtc atggagttct ccaccttctc ttcaaaataa
     8941 gtgcgtggat ccgttcctga gaaatagagc tttcgcaagt cagcagtgat ctgtctggca
     9001 tccttcaaag acagggatgg gtatcgacca agcccaagtc gattaggctt gccatgccag
     9061 cgatagcggt actggaactg gatgaccccc ttcggtgaaa ttcgtacgct gaggccatcg
     9121 gcatcagcca cttcttgtgg gcccgaatat ggtttaccat aaatagtacg cagttttgtg
     9181 tcgcttatag ccataaataa tatttggtac gctcaaaaat aatggtttgg tacacatctg
     9241 gtacacaata tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac
     9301 atactcaggt gaaaaaatgg ttgtttttcg agttataaca gacagttaat taactacagt
     9361 ggacaatgct agacaatgac agacacagat aaacaaccta ccttcctctt tcacgattac
     9421 gaaacctttg gaacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcact
     9481 gataacgaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgcggatgac
     9541 tatttacccc agcctggagc agtattaatt accggtatta ccccgcagga agcacgggcg
     9601 aaaggagaaa acgaagccac gtttgccgcc cgtattcact cgctttttac cgtaccgaag
     9661 acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt
     9721 tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat
     9781 ttactggatg ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa
     9841 aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggtattgaa
     9901 catagcaacg cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg
     9961 gtaaaaacgc gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa
    10021 ctgatggcgt tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt
    10081 ggggcatggc gtggcaatac cagctgggtg gcaccgctgg cgtggcatcc tgaaaatcgc
    10141 aatgccgtaa ttatggtgga tttggcagga gacatttcgc cattactgga gctggatagc
    10201 gacacattgc gcgagcgttt atataccgca aaagccgatc ttggcgataa cgccgccgtt
    10261 ccggttaagc tggtgcacat caataaatgt ccggtactgg cccaggcgaa tacgctacgc
    10321 ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctaaaaatt
    10381 ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtggcga tattcgcgga agccgaaccg
    10441 tttacgcctt cagataacgt ggatgcacag ctttataacg gctttttcag tgacgccgat
    10501 cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc
    10561 acttttgttg ataaacgaat tgaaaaactg ttgttcaatt atcgggcgcg caacttcccg
    10621 gggacgctgg attatgccga gcagcaacgc tggcttgagc accgccgcca ggtcttcacg
    10681 ccagagtttt tgcagggcta cgctgatgaa ttgcagatgc tggcacaaca gtatgccgat
    10741 gacaaagaga aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa
    10801 tttgaagcct ccgccgctgg tacggcggag gacaatgttg agtcaggctt tttgaacggt
    10861 gatgctccac gctgcatcgc caatctgctg ataatcggtg atggcatgtc cttcttctgc
    10921 cgcccactgt gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc
    10981 gccgctggcc atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg
    11041 cgtcaccaca tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag
    11101 ttgccagtcg cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc
    11161 cgagaatcat aaatacaagg ccaatccagc cctgccaggt catcattgca gtcatgacca
    11221 gtccattacc gatagagcaa ccacctgcaa tgctggcacc gaagcccatc agtacaccac
    11281 ctaacccgct acgtaatgtt gtttgtgcat cagcagcgcg aacgcggaac tcacggctcg
    11341 ctttggcggc aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa
    11401 tgtatttcac gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgatgc
    11461 ctaacccaaa catacgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac
    11521 cgatgagtac agctgtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc
    11581 cggttcggcg cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca
    11641 ccacccagag cgttatcacc agcaaaacgg caaccaacgg ccacacagac aaattaaaag
    11701 tatcagcaat agagttatgt tcagtagtgt agtgctgcaa ggtttgattt aaaccactgg
    11761 cgtgtggaga acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc
    11821 cgatcaatcc ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa
    11881 ttcccagccc gaaaagatac ccacctataa cagtaccaag ccacgggaac gccccggctt
    11941 cgtaagtcag taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg
    12001 caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac
    12061 ctgtcaggca aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc
    12121 cgcttaatat cattgaaaac ataaattaat taaccagatg aatgttaatg aggaaattat
    12181 tcatgactgg tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt
    12241 aatgaggatt tcgataaaaa agaataaaaa aaccggagcc aaatattcat tcgactccgg
    12301 ttgattatta gaaaagatga ttatgctacg tcttcgtact gcggaaccgg gttacggaag
    12361 ctcttggtga cgcaagccag gtaaatcaga ccgatagcag cccagatcag acccagaacc
    12421 attgagcttt cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc
    12481 aggaacagat actggaagtg atctttcagc gtcttgttac gcttctcacg gatccagaac
    12541 tgcgagataa ccgacaggtt aacgaaggtg aacgccacca acgcaccaaa gttaatcagc
    12601 gccgtagcca ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg
    12661 atgttcatcg ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaac
    12721 acgccgtcac gacccattac gtacatcaga cgcgctacgc ctgcgtgcgc cgccataccg
    12781 gacgccagta cggtaatggt ggagaagatc agcgcgccaa cttggaacgc tttacccgca
    12841 acgtacagca tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg
    12901 aagtacagct gcaggaagta agttgcaaag atgaagatca tgccgccaat cagcgcggtc
    12961 aggaaaatcg cacgcgggat cacgcgctct gcatctttgg tttcttccga caggttgctg
    13021 atgccgtcaa agccggtaaa ggagaagcac aggattgtcg ccccggtaat catcgggata
    13081 acatgcgcat cgccagacca gaacggacga gtgctcgcca gcgtacccgc gccttcacct
    13141 tcaaatactc cgtaaactac catgcccaga attaccgcga tcagcactac ctgcaacacc
    13201 acgatgacgg tgttgaagtt cgctacggat ttcagactac gcaggttaaa ggcggtcata
    13261 aaagccacca gcgccaccac aaacatccac gatggaatgg aaggcaccag agcttcaaaa
    13321 tagattttcg ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac
    13381 caacccacca taaagccgac ggtcgggcta atggatttct gggcgtaagt gtatgcagag
    13441 ccagcagaag gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca
    13501 atcaatgcga acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca
    13561 aaagtatcaa acagcgtcat cggctgcata taggcaagac ccatcattac aaccggaact
    13621 aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg
    13681 gtcattctcc tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga
    13741 ttcctcagcg acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc
    13801 tcttggtatg aatgattggt ttgaagcaaa aaataaccga cgctgatgaa acgtcggttt
    13861 ttagtcattt tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg
    13921 agaggcttgt cataataatt tttctttaaa tggctgattt tccgtcatca gattttgcat
    13981 aaacaccggc gatcgcacta tttgctaaaa tttcatctcg ccaccagccg ctggcaagcc
    14041 ctgcggtctg ttcgttccag ccaatccaga cgggttcaaa gagagtttgg gcaaccactt
    14101 tcttctcgat taacgcgcca ctatcgagaa aacgttgcgc cagataacgg ggtaaatagc
    14161 cgcagccaag gccgctaatt tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg
    14221 cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtatccccca
    14281 ccacaatcgc ccgatatcgc ttaatgatac gacggtttaa cggttcttct tccagggcta
    14341 acggatgatg tggcgcgacc gcaaagactt gttccagatc gcctaaccgc gagaaaccaa
    14401 actcactgga agatggtggc tcatgcatcg cgccaacaat aatatccgct cgcccctgag
    14461 tcagggcatc ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacactat
    14521 gatgttgata aaaagcttca atgagtggcg caagcagaga aaaagggaag gtgtcgtcca
    14581 caccaatcac cagctcgttt tcccagcctt catgcagttt gatcgcctgc ttttccagtt
    14641 cccgcacggt atgcagaact tcccgccctt tctctaataa catttttccg gtgcgggtga
    14701 atttagcgcg gtgtccgcta cgatcaagca attgaatatt aaggtcgctt tccaacttgt
    14761 gaacggtgta actcagagcg gacggcgtct tgtagagttt ggccgacgcc gccgcaaaac
    14821 tgccttcttt ttctaaggca tcgaggatca tcaacacatc cagcagtggt ttcatacacg
    14881 cccccttgca gtgcatgcgg cgtcctgctg gcgtgatcgt ttactccagc ggcattacca
    14941 gcggatcggg atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa
    15001 tcttaccttt gccgctgtcc agactatttc tgaactgcgg aggttccagc cccagtaaac
    15061 gggccatctg cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga
    15121 tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc gccaataaca tcttctaaat
    15181 ggactaaatt aacaccatgt tcaccatcag gggcggtttt tccggcaaag aagcgtccgg
    15241 gatgacgtcc cggtccgacc agccccgcaa gacgcagaat atcgaccgaa gtaccgggta
    15301 aattgtgcag ccagtcttcg aactcttcta atactcgtcc actgttggtc accggattac
    15361 gcggggtagt ttctttcacc gtgccttgcg catcgccata gacagatgtt gagctggtga
    15421 aaataatgcg gggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta
    15481 aatagaactc atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg
    15541 tatccatcag ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca
    15601 gataactatc aatgccactc attcgggccg cttcgacacc atcttgcgtg gttttactcc
    15661 cggtgacttg ccagcctcgc gctgaaagtg acatcgccag cggcatgcct aaccacccta
    15721 acccgacaat tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc
    15781 tacgctagcg tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa
    15841 cgacatgctg caatgagcag tgaatcgtcg gtaaccgcgt ataccagacg gtgctcctct
    15901 gtaatgcgtc gggaccagaa gcctgacaaa ttatgcttca ggggttctgg cttcccctta
    15961 ccttcaaatg gtgttctgcg ggtatctttg ataagttcaa tgatcttttt aacaattcgc
    16021 ttatctgttt cctgccagta cagataatca tcccatgact cctcagacca gattagtttc
    16081 actcaataat gtccttttcc gttcctttgc ctgatttcag gctatcgatt gagtccatca
    16141 atctccgggc gttagcgggg gagcgcagta gataagccgt ctcttccagc gagttgtatt
    16201 cttcgagtga catcagaaca caagcctctc cattctgacg agtaataagg atcggggcat
    16261 gatcttcaac ggctttcatc attgttgccg acaaattctg gcgcgcttcg ctgtagctaa
    16321 ttgtacgcat ttcaatctcc tcttttgtac agttcattgt acaatgatag gcgttaatta
    16381 actatttatt aattagtctg tagatcaaga cattgtcagt gagacgaaaa atccaggttt
    16441 cgctattttg ggtgccgtca ggttagggga cactcctctt agccccctcc tttccccgct
    16501 cattcattaa acaaatccat cgccataaaa tatataaaaa agcccttgct ttctaacgtg
    16561 aaagtggttt aggttaaaag acatcagttg aataaacatt cacagagact tttatgacac
    16621 gcgttcaatt taaacaccac catcatcacc atcatcctga ctagtctttc aggcgatgtg
    16681 tgctggaaga cattcagatc ttccagtggt gcatgaacgc atgagaaagc ccccggaaga
    16741 tcaccttccg ggggcttttt tattgcgcgg ttgataacgg ttcagacagg tttaaagagg
    16801 aataagaaaa tgacagacaa cactcgttta cgcatagcta tgcagaaatc cggccgttta
    16861 agtgatgact cacgcgaatt gctggcgcgc tgtggcatta aaatcaatct tcacacccag
    16921 cgcctgatcg cgatggcaga aaacatgccg attgatattc tgcgcgtgcg tgacgacgac
    16981 attcccggtc tggtaatgga cggcgtggta gaccttggga ttatcggcga aaacgtgctg
    17041 gaagaagagc tgcttaaccg ccgagcccag ggtgaagatc cgcgctactt taccctgcgt
    17101 cgactggatt tcggcggctg ccgactttca ctggcaacgc cggttgatga agcctgggat
    17161 ggcccgctct ccttaaacgg taaacgtatc gccacctctt atcctcacct gctcaagcgt
    17221 tatctcgacc agaaaggcat ctcttttaaa tcctgcttac tgaacggttc tgttgaagtc
    17281 gccccgcgtg ccggactggc ggatgcgatt tgcgatctgg tttccaccgg tgccacgctg
    17341 gaagctaacg gcctgcgcga agtcgaagtt atctatcgct cgaaagcctg cctgatacaa
    17401 cgcgatggcg aaatggaaga atccaaacag caactgatcg acaaactgct gacccgtatt
    17461 cagggtgtga ttcaggcgcg cgaatcaaaa tacatcatga tgcacgcacc gaccgaacgt
    17521 ctggatgaag tcatcgccct gctgccaggt gccgaacgcc caaccattct gccgctggcg
    17581 ggtgaccaac agcgcgtagc gatgcacatg gtcagcagtg aaaccctgtt ctgggaaacg
    17641 atggaaaaac tgaaagcgct gggtgccagt tcaattctgg tcctgccgat tgagaagatg
    17701 atggagtgat cgccatgagc tttaacacaa tcattgactg gaatagctgt actgcggagc
    17761 aacaacgcca gctgttaatg cgcccggcga tctccgcttc tgaaagcatt acccgcactg
    17821 ttaacgatat tctcgataac gtgaaagcac gcggtgatga cgcccttcgg gaatacagcg
    17881 cgaagtttga taaaaccacg gttaacgcgc tgaaggtgtc tgctgaggag attgccgccg
    17941 ctagcgaacg cctgagcgac gagctaaaac aggcgatggc ggtggcagta aaaaatattg
    18001 aaaccttcca cactgcgcaa aaactgccgc cggtagatgt agaaacgcag ccaggcgtgc
    18061 gttgccagca ggtcacgcgc ccggtcgctt cagttgggtt gtatattcct ggcggctccg
    18121 ccccgctctt ctcaacggta ttaatgctgg cgactccggc gcgtattgcg ggctgtaaaa
    18181 aagtggtgct gtgctcaccg ccgccgattg ccgatgagat cctttatgcg gcgcagctgt
    18241 gcggtgtgca ggacgtgttt aacgtcggcg gcgcacaggc cattgccgcg ctggcgtttg
    18301 gtacggaatc tgtgccgaaa gtggacaaaa tcttcgggcc aggcaacgcc tttgtcaccg
    18361 aggcaaaacg ccaggtaagc cagcgtctgg acggtgcggc gatcgatatg cccgcaggcc
    18421 cgtcggaagt gctggtgatt gccgacagcg gcgctacgcc agatttcgtg gcttctgatt
    18481 tgctttctca ggcagaacac ggcccggact ctcaggtgat tttactgacg cccgatgctg
    18541 atatggcgcg tcgcgttgcc gaagctgtcg aacgccagct ggcagaactg ccgcgtgccg
    18601 aaaccgcacg tcaggcactg agcgccagcc gcctaatcgt gactaaagat ttagcgcagt
    18661 gcgtagagat ttccaaccag tacggcccgg agcacctgat cattcagacc cgcaacgccc
    18721 gcgaactggt cgatggcatc accagcgccg gttcggtatt tcttggtgac tggtcaccgg
    18781 aatcggcagg tgattacgcc tccggcacca accacgttct gccgacttac ggttacaccg
    18841 ccacctgttc cagcctcggg ctggcggatt tccagaagcg gatgaccgtg caggaactgt
    18901 cgaaagtagg tttctccgcg ctggcttcga ccattgaaac actggccgcc gccgagcgcc
    18961 tgaccgccca caaaaatgcc gttactttgc gtgttaacgc ccttaaggag caagcatgag
    19021 caccgtgact attaccgatt tagcgcgtga aaacgtccgc aacctgacgc cgtatcagtc
    19081 ggcgcgtcgt ctgggcggta atggcgacgt ctggctgaac gccaacgaat atcccacagc
    19141 cgtggagttt cagcttactc agcaaacgct caaccgctac ccggaatgcc agccgaaagc
    19201 ggtgattgaa aattacgcgc aatatgcagg cgtaaaaccg gaacaggtgc tggtcagccg
    19261 tggcgcggac gaaggtattg aactactgat tcgcgctttt tgcgaaccgg gtaaagacgc
    19321 catcctctac tgcccgccaa cgtacggcat gtacagcgtc agcgccgaaa ccattggcgt
    19381 cgagtgccgc acagtgccga cgctggacaa ctggcaactg gacttacagg gcatttccga
    19441 caagctggac ggcgtaaaag tggtctatgt ttgcagcccc aacaacccaa ccgggcaact
    19501 gatcaatccg caggattttc gtaccctgct ggagttaacg cgcggtaagg cgattgtggt
    19561 tgccgatgaa gcctatatcg agttttgccc acaggcatcg ctggctggct ggctggcgga
    19621 atatccgcac ctggctattt tgcgcacact gtcgaaagct tttgctctgg cgggccttcg
    19681 ttgcgggttt acgctggcaa acgaagaagt catcaacctg ctgatgaaag tgatcgcccc
    19741 ctacccgctc tcgacgccgg ttgccgacat tgcggcccag gcgttaagcc cgcaggggat
    19801 cgtcgccatg cgtgagcggg tggcgcaaat tattgcagaa cgcgaatacc tgattgccgc
    19861 actgaaagaa atctcctgcg tggagcaggt ttttgactct gaaaccaact acattctggc
    19921 gcgctttaaa gcctccagtg cagtgtttaa atctttgtgg gatcagggca ttatcttacg
    19981 tgatcagaat aaacaaccct ctttaagcgg ctgcctgcga attaccgtcg gaacccgtga
    20041 agaaagccag cgcgtcattg acgccttacg tgcggagcaa gtttaatgag tcagaagtat
    20101 ctttttatcg atcgcgatgg aaccctgatt agcgaaccgc cgagtgattt tcaggtggac
    20161 cgttttgaca aactcgcctt tgaaccgggc gtgatcccgg aactgctgaa gctgcaaaaa
    20221 gcgggctaca agctggtgat gatcactaat caggatggtc tgggaacaca aagtttcctg
    20281 caggcggatt ttgatggccc gcacaacctg atgatgcaga tcttcacctc gcaaggcgtg
    20341 cagtttgatg aagtgctgat ttgtccgcac ctgcccgccg atgagtgcga ctgccgtaag
    20401 ccgaaagtaa aactggtaga gcgttatctg gctgagcaag cgatggaccg tgccaacagt
    20461 tatgtgattg gcgatcgcgc gaccgacatt caactggcgg aaaacatggg cattaatggt
    20521 ttacgctatg accgcgaaac cctgaactgg ccaatgattg gcgagcagct cactaaacga
    20581 gaccgttacg cccatgtggt gcgcaacacc aaagagacgc aaattgacgt ccaggtgtgg
    20641 ctggatcgcg aaggtggcag caagattaac accggcgttg gcttctttga tcacatgctg
    20701 gatcagatcg ccacccacgg cggtttccgg atggaaatca acgtcaaagg cgacctctat
    20761 atcgacgatc accacaccgt cgaagatacc ggcctggcgc tgggcgaagc gttaaaaatt
    20821 gctcttggcg acaaacgcgg tatttgccgc tttggttttg tgctgccgat ggacgaatgc
    20881 cttgcccgct gcgcactgga tatctctggt cgcccgcacc tggaatataa agctgaattt
    20941 acctaccagc gcgtgggcga tctcagcact gaaatgatcg agcacttctt ccgttcgctc
    21001 tcttacacca tgggcgtgac gttgcacctg aaaaccaaag gtaaaaacga tcatcaccgt
    21061 gtagagagcc tgttcaaagc ttttggtcgc accctgcgcc aggccatccg cgtggaaggc
    21121 gataccctgc cctcgtcgaa aggagtgctg taatgaacgt ggtgatcctt gatactggct
    21181 gcgccaacct gaactcggtg aagtctgcca ttgcgcgcca cggttatgaa cccaaagtca
    21241 gccgtgaccc ggacgtggtg ttgctggccg ataaactatt tctacccggc gttggcaccg
    21301 cacaagcagc gatggatcag gtgcgtgagc gcgaactgtt tgatctcatt aaagcctgta
    21361 cccaaccggt gctgggcatc tgcttaggga tgcaactgct ggggcggcgc agcgaagaga
    21421 gcaacggcgt cgatttgctg ggcatcatcg acgaagacgt gccgaaaatg accgactttg
    21481 gtctaccgct gccccatatg ggctggaacc gcgtttaccc gcaggcaggc aaccgcctgt
    21541 ttcaggggat tgaagacggc gcgtactttt actttgttca cagctacgca atgccagtca
    21601 atccgtggac catcgcccag tgtaattacg gcgaaccgtt caccgcggcg gtacaaaaag
    21661 ataacttcta cggcgtgcag ttccacccgg agcgttctgg tgccgctggc gctaagttgc
    21721 tgaaaaactt cctggagatg tgatgattat tccggcatta gatttaatcg acggcaccgt
    21781 ggtgcgtctc catcagggcg attacggcaa acagcgcgat tacggtaacg acccgctgcc
    21841 gcgcttacag gattacgcca cgcagggtgc cgaagtgttg catctggtgg atctgaccgg
    21901 ggcaaaagat ccagcaaaac gtcaaatccc gttgattaaa accctggtcg cgggcgttaa
    21961 cgttccggtg caggttggcg gcggcgtgcg tagcgaagaa gacgtggcgg cgttactgga
    22021 agcgggcgtt gcgcgcgtgg tggtcggctc caccgcggtg aaatcaccag aaatggtgaa
    22081 aggctggttt gaacgtttcg gtgccgatgc cttagtgctg gcgctggatg tccgtattga
    22141 cgagcaaggc aacaagcagg tggcagtcag cggctggcaa gagaactcgg gcgtttcact
    22201 ggaacaactg gtggaaacct atctgcccgt cggcctgaaa catgtgctgt gtaccgatat
    22261 ctcgcgcgac ggcacgctgg caggttctaa cgtctcttta tatgaagaag tgtgcgccag
    22321 atacccgcag gtggcatttc agtcttccgg cggtattggc gacattaacg atgttgctgc
    22381 cctgcgtggc actggcgtgc gcggcgtaat agttggtcgg gcattactgg aaggtaaatt
    22441 caccgtgaag gaggccatcg catgctggca aaacgcataa tcccatgtct cgacgttcgt
    22501 gatggtcagg tggtgaaagg cgtacagttt cgcaaccatg aaatcattgg cgacatcgtg
    22561 ccgctggcaa aacgctacgc cgaagaaggt gcagacgaac tggtgttcta cgatatcacc
    22621 gcttccagcg atggccgtgt ggtagataaa agctgggtat ctcgcgtggc ggaagtgatc
    22681 gacattccgt tttgtgtggc gggtgggatt aagtctctgg aagatgccgc gaaaattctt
    22741 tcctttggcg cggataaaat ttccatcaac tcccctgcgc tggcggaccc gacgttaatt
    22801 actcgcctgg cggatcgatt tggcgtgcag tgtattgtgg tcggtattga tacctggtac
    22861 gacgctgaaa ccggtaaata tcatgtgaat caatataccg gcgatgaaag ccgcacccgc
    22921 gtcactcagt gggaaacgct cgactgggta caggaagtgc aaaaacgcgg tgccggagaa
    22981 atcgtcctca acatgatgaa tcaggacggc gtgcgtaacg gttacgacct cgaacaactg
    23041 aaaaaagtgc gtgaagtttg ccacgtcccg ctgattgctt ccggtggcgc gggcaccatg
    23101 gaacacttcc tcgaagcctt ccgcgatgcc gacgttgacg gcgcgctggc agcttccgta
    23161 ttccacaaac aaataatcaa tattggtgaa ttaaaagcgt acctggcaac acagggcgtg
    23221 gagatcagga tatgttaaca gaacaacaac gtcgcgaact ggactgggaa aaaaccgacg
    23281 gacttatgcc ggtgattgtg caacacgcgg tatctggcga agtgttaatg ctgggctata
    23341 tgaacccgaa agctctggac aaaaccatcg aaagcggcaa agtcaccttc ttctcgcgca
    23401 ctaaacagcg actgtggacc aaaggcgaaa cgtcgggcaa tttcctcaat gtggtgagta
    23461 ttgcgccgga ctgcgacaac gacacgttac tggtgctggc gaatcccatc gggccaacct
    23521 gccacaaagg caccagcagc tgcttcggcg acaccgctca ccagtggctg ttcctctatc
    23581 aactggaaca actgctcgcc gaacgtaaat ctgccgaccc ggaaacctcc tacactgcca
    23641 aactgtatgc cagcggcacc aagcgcattg cgcagaaagt gggcgaagaa ggcgtggaaa
    23701 ccgcactggc agcaacggta catgaccgct ttgagctgac caacgaggca tctgatttga
    23761 tgtatcacct gctggtgttg ttgcaggatc aggatctgga tttaacaacg gtgattgaga
    23821 acctgcgtaa acggcatcag taagttgcgg gctgagcgga tgcgatattg ttgtcgcatc
    23881 cggccaaaaa aatgggcaag gtgtcaccac cctgcccatt ttctttaaaa ccgaaaagat
    23941 tacttcgcgt tgtaattacg cagagcgtta cgccccagca caatccccgc gccaaccatg
    24001 ccgcccagca gcaccgccag aatcaaggta attgcctttt tcgggctatc gcgacgaaca
    24061 ggtaacgtcg gtttcattac atagcggtaa gcatgaatat caagatcatc aacttttaag
    24121 ctttcgatat ccagcaggtt ttgacgtgtc tgatagtagt ttggtgagaa taccaacgga
    24181 cgggtcgcct cgtgcttaat catcgactcc agcgcttcgc tccccaaaag gaacaacgta
    24241 tcttgtgtca catcttcagt ctgctgaacc tgtggctttg tcacctgcgc ctgattcgca
    24301 tactgcaacg cttcctgaat ctgacggata cgcagatctt tctgctcctg cgcgactact
    24361 tcctgagttt tcagagaatc ctgcaagttt ttcatacgca gcgcgatgtt gtctttcagg
    24421 tctttttcca gttcttcatt aacctggtcg tcaacttgct ggatatactt cgccaactgt
    24481 ttctgcgcac cttcaggtgt ttcaccaaca taagacaccg ccagtggtaa cggctggttt
    24541 ttaacggtag gctcaatggt cagcttctct gcttcttcct ggttatccag cgtttccgct
    24601 aatgctgaga atgcagtgct gtaacgtccg ataagtgaag tctgaatgtc ggagactttc
    24661 ggtacagccg atccgtagat cacattcatc gcgttggtat aaccagcgat ttgccccaca
    24721 tcaggttgag taacgatagc tgtggaagtc cacttttctt tggcaacggc cagataccct
    24781 atagccaggg caatagcaac aatgacagag atgataattg tcatcttgcc acgccacaac
    24841 tgcaccagta aatcaatcaa atcaatctgt tccgggtcat ggttttgccc agaaacatta
    24901 ttattttcta ctctcatcat taccctaacg gacgaaataa ttttaagtcg ccatagttta
    24961 gcgataatcg ccatgaatgc tataggataa atgataaaaa taatgaatta ttacaaaggg
    25021 aacataagag aatcagggcg aaaatcgccc tgataacagg atgttagtcg cttccaaaga
    25081 gatcgcgggt gtagactttg tctgccacat ccttaagttc ttctgccata cggttggaga
    25141 taatgacgtc ggcttgttgt ttgaaggtgg cgagatcacg ttccaggcga gagttgaaga
    25201 atgaatcttc tttcatcacc ggttcgtaga tgatcacttc aacgcctttc gccttgatac
    25261 gtttcataat cccctgaatg gaagacgcac ggaagttatc tgacccgctc ttcataatca
    25321 gacgataaat acccaccact tgcggtttgc gtgacaaaat ggcatcggca ataaagtctt
    25381 tacgtgtgcg gttagcatcg acaattgccg agatcaggtt attcggcaca gactggtagt
    25441 tcgccagtaa ctgcttggta tctttcggca gacaataacc accataacca aacgacggat
    25501 tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat
    25561 tcagccctaa actttctgca tagctatcca gttcattaaa gtatgctacg cgcatcgcca
    25621 gataagtatt agcgaaaagt ttaatcgctt ctgcttcggt tgaatcggta aacagggttg
    25681 ggatattttg cttaatcgcg ccttcctgta ataacgccgc aaaacgttct gcgcgttctg
    25741 aacgctcacc gatgacaata cgtgacggat ggagattatc gtaaagagct ttaccttcac
    25801 ggagaaattc cggagagaaa ataaggttat cgatacccag acattcttta attgatttgg
    25861 tgaaacctac cggaattgta gatttaatca ccatgacagc attaggatta atttctgtca
    25921 catcgcgaat gaccgcttcc acgcttgagg tattaaaata atttgttttc ggatcataat
    25981 cggtaggtgt ggcaataata acgtaatcgg catttttata cgcgtcatac ttatctgtcg
    26041 tagcgcggaa attgagatct ttagtcgcca gatactcttc aatctcctta tcaacaatcg
    26101 gtgactgctt cttgttaagc atgtccactt tggcctgaac gatatccagt gcaaccactt
    26161 cgtgattttg cgcaatcaga ataccatttg aaagaccaac ataacctgtt cctgaaattg
    26221 ttattttcat tagctctgac ttcttccggt taaacattta gagtggtcat taatccacca
    26281 ccacaacttc atctactgcg ggatttttaa cgctgatgta gatgaactgt caaggcagcg
    26341 atcctgctgt gcggcgcttt attatatcgc gtttttaact ataaattata aaaaaaggcc
    26401 ataacctgcc gctttgtata ataaaaaagc ccggagggtt tctccgggcc ttgcattgat
    26461 taattggttt aaatcagatt aatccagcca ttcggtatgg aagacacctt ctttatcaat
    26521 gcgcttataa gtatgcgcac cgaaatagtc acgctgtgcc tggatcaggt tcgcaggcag
    26581 aacagcagca cggtagctat cataataggc aaccgcagcg gcgaaggtcg gaaccgggat
    26641 accgttctgt accgcataag caacgacatc gcgcagcgcc tgctggtagt catcggcaat
    26701 ttgtttgaag tacggagcca gcagcaggtt agcgatctgc ggattttcgg cataggcatc
    26761 ggtgattttc tgcaggaact gggcacggat gatgcagcca gcacggaaaa tcttcgcgat
    26821 ttcgccgtag ttcagatccc agttgtactc ttcagacgcg gcgcgtagct gagagaagcc
    26881 ctgagcgtaa gaaacgattt tgcccagata cagagcacga cgaacttttt cgatgaactc
    26941 agtcttgtcg cctgctggct gtgcttgcgg gccagagaga actttagatg cggcaacacg
    27001 ctgatctttc agagaagaga tataacgtgc aaacacagac tcggtaatca gcgacagcgg
    27061 ttcgccgaga tccagcgcac tctggctggt ccatttgccc gtacctttgt ttgctgcttc
    27121 atccagaatc acatcaacca ggtagttacc gtcttcatct tttttggtga agatatcttt
    27181 ggtgatgtcg atcaggtagc tgcttagttc accgttattc cactcggtaa acgtctgcgc
    27241 cagttcttcg ttggaaagat tcaggccacc tttaagcaga gaataggctt cagcaatcag
    27301 ctgcatatcg ccgtattcaa taccgttgtg aaccatcttc acatagtgac ctgcaccatc
    27361 ggcaccaata taggtaacgc atggctcacc gtcttcagct actgcggcga ttttggtcag
    27421 gattggtgca accagctcat aggcttcttt ctgcccacca ggcataatgg aaggaccttt
    27481 cagcgcgcct tcttcaccgc cggaaacacc ggtaccgata aagttaaagc cttcggcaga
    27541 aagctcgcgg ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat caatgatgat
    27601 gtcgccttta tcgaggtatg gcttgagaga atcaatagca gcatccgtgc ctgcacctgc
    27661 tttcaccatt aacaggatgc gacgaggcgt ttccagagat tcaacaaact ctttcaccgt
    27721 atagtaagga accagtttct tgcctggatt ttcggcaatc acttcttcgg tcttttcacg
    27781 ggaacggttg aaaatagaga cggtataacc acggctttcg atgttgagcg caaggttgcg
    27841 ccccatcact gccataccga cgacgccgat ctgttgcttt gacattgttt actcctgtca
    27901 ggggggtcac ca
//
LOCUS       DAZFZC010000070        27483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFZC010000070 DAZFZC010000000
VERSION     DAZFZC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.070"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..259
                     /locus_tag="ACHKXP_003281"
     CDS             71..259
                     /locus_tag="ACHKXP_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648231.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            554..2179
                     /locus_tag="ACHKXP_003282"
     CDS             554..2179
                     /locus_tag="ACHKXP_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648232.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGLEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSKIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNVALATEQIVSPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            2176..3444
                     /locus_tag="ACHKXP_003283"
     CDS             2176..3444
                     /locus_tag="ACHKXP_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7648233.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKAAGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3459..3737
                     /locus_tag="ACHKXP_003284"
     CDS             3459..3737
                     /locus_tag="ACHKXP_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFO7648234.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPIFFLDRFTAESGSKTYTNKPEGK
                     TLQAVCSLFPWNNVFKDRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            3743..4360
                     /locus_tag="ACHKXP_003285"
     CDS             3743..4360
                     /locus_tag="ACHKXP_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648235.1"
                     /translation="MYGLSITKPDGSLWISPGFTPQCLINKGTIPATEKAFFKTSIPS
                     GKSCFFFIRTENKADVMYTHEQIDGYHALRLHQIVRGTNPGVTTVYAFANMVTQPSEY
                     GIAMYNPSGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINSSIYD"
     gene            4440..5177
                     /locus_tag="ACHKXP_003286"
     CDS             4440..5177
                     /locus_tag="ACHKXP_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7648236.1"
                     /translation="MKSIMKFFACAVLVMSCLTAQVNAASGDSTVSLGFAHIRFPGLK
                     DFVRDAGVYNRDTFRNVVNVNHFNSSGEYENAIARGHDGTAKSPLGMSIRYRYEITDE
                     LGVIASFTWARSMTNAQAFIDVKPSDPSREVKNPAASARTDIRANYWSLLSGPSWRFN
                     EYLSVYAMAGMGVAKVTTDLKINDNLNHGAGSFSESNSTKKTSIAWSVGAQFNFNESV
                     TMDVTYESSGSGDWKTDGLFAGIGLKF"
     gene            5410..5550
                     /locus_tag="ACHKXP_003287"
     CDS             5410..5550
                     /locus_tag="ACHKXP_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7648237.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            5607..6008
                     /locus_tag="ACHKXP_003288"
     CDS             5607..6008
                     /locus_tag="ACHKXP_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648238.1"
                     /translation="MAPVSGERMDDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSAGVERYLPEVKTLTSLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            6102..6755
                     /locus_tag="ACHKXP_003289"
     CDS             6102..6755
                     /locus_tag="ACHKXP_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648239.1"
                     /translation="MGGKKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGETRRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYIAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGSGGSKTPDAGANAIQSQAQNWRL"
     gene            6758..7204
                     /locus_tag="ACHKXP_003290"
     CDS             6758..7204
                     /locus_tag="ACHKXP_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648240.1"
                     /translation="MEYGKYETLARAGYSGADRPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPNSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQQLSVGGQMMTA"
     gene            7215..7466
                     /locus_tag="ACHKXP_003291"
     CDS             7215..7466
                     /locus_tag="ACHKXP_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO7648241.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            7477..8742
                     /locus_tag="ACHKXP_003292"
     CDS             7477..8742
                     /locus_tag="ACHKXP_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648242.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYALQEREFNANQNYRNASLGMELQRLQLQKY
                     NQRRLEYNDMLARDQSVMVALGKAIDAGDHDAASRLFGKLSDANPIKIISTEGYAAKA
                     GQAVNNLQKIFDDKPDRAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            8812..17166
                     /locus_tag="ACHKXP_003293"
     CDS             8812..17166
                     /locus_tag="ACHKXP_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO7648243.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDSNRWKD
                     NSTSFSLGDVLPTMGKGFARSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKVTGKAVSDGAKSSDEWLTSK
                     MSPGALRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKTLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLLNLAMRGTANTVKSGIVRNATEQGTINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAALGAAMGAPFGAAAGYRGKRQAADEAAMREAESVPQDESA
                     PQPEPVDPVAQHRESMQGMNREQLLEQYADADMAHEGDTSAVHRREAASQLLNELDEQ
                     AKRQAVMDELKAKPRHELLEEYRKLSQKEGRTDTEEQQLQAIRDVLRPQREARPEAQP
                     QPENADDGDGSIYPTVRFRDPDEVRIEINESGASRPAERIEKVRPDNRYFTDEKSALG
                     SDVFRNASATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITDADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPENAAGR
                     QGEILEGETVRRGLPSPDEQNATAPVREGLPAPESQRGVDMPQPESLPRMVRDSLPEL
                     AQQAEARRQTGDNRQTITDVPDTEVPVPVDKPSTHQQARGAKIEDFGEEIKGAAKHRY
                     AQLAEAMGKTLEDGEYATQPLSKLFPKPDYARLEKEGVDSDTLAMMALYRSEIPTRTS
                     RNMQKWISIVKSGREATAGMLEGKIPAAKLADMMDSKPGLRSMSDTWKLLRTLSALQI
                     DKASGYRVRSGVYSFVGGKRYDPPQMMYSLRDSKGRDLFFSESRDELLKKAKAYFDEQ
                     GSRERETPATSADDRITFDVYRHKASGDIFIGYGKNRQKLKGGFESARDAHDYVRTHR
                     DELVNQVKALREVSREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGNYVES
                     PRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVAIN
                     LTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGRYVDA
                     EYPVRQEVYDAFKGVMKAINSSDMLRRSERLDGVRSKPYWSTDVEMAARAFERYVQDK
                     ARMAGVENDYLVNIRKADDHGQPDTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGV
                     AFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQ
                     KEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVV
                     TPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKF
                     VAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGLRVREGYTPREDGAGASS
                     QYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAPDLPL
                     VISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGD
                     PVVSAVHMKATDKRIEINKVASVYGTKGGMNKANSLDKAGLTLYRKEKLSRDNPQYSG
                     LQLPKEERSYRGSVDKILYPEDIRNGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKA
                     VQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADL
                     DEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGII
                     DDMLARRRELIRQAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGR
                     ESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDD
                     RPDTMRTIAERKDQETGETIREAVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMR
                     AMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRS
                     DGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGW
                     FNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHA
                     RDTGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGE
                     GPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERN
                     LVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGA
                     FSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQL
                     GRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKYWVDYISGGTGRFISKTTDA
                     AVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTA
                     FIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAV
                     DRFNREYNKKVGVE"
     gene            complement(17388..18320)
                     /gene="yfdC"
                     /locus_tag="ACHKXP_003294"
     CDS             complement(17388..18320)
                     /gene="yfdC"
                     /locus_tag="ACHKXP_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO7648244.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLIRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNYKNPA"
     gene            18614..19369
                     /gene="mlaA"
                     /locus_tag="ACHKXP_003295"
     CDS             18614..19369
                     /gene="mlaA"
                     /locus_tag="ACHKXP_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO7648245.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(19551..20610)
                     /gene="yfdF"
                     /locus_tag="ACHKXP_003296"
                     /pseudo
     CDS             complement(19551..20610)
                     /gene="yfdF"
                     /locus_tag="ACHKXP_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
     gene            complement(20976..22316)
                     /gene="fadL"
                     /locus_tag="ACHKXP_003297"
     CDS             complement(20976..22316)
                     /gene="fadL"
                     /locus_tag="ACHKXP_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO7648246.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            22688..22972
                     /gene="yfcZ"
                     /locus_tag="ACHKXP_003298"
     CDS             22688..22972
                     /gene="yfcZ"
                     /locus_tag="ACHKXP_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO7648247.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            23153..24463
                     /gene="fadI"
                     /locus_tag="ACHKXP_003299"
     CDS             23153..24463
                     /gene="fadI"
                     /locus_tag="ACHKXP_003299"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO7648248.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            24463..26607
                     /gene="fadJ"
                     /locus_tag="ACHKXP_003300"
     CDS             24463..26607
                     /gene="fadJ"
                     /locus_tag="ACHKXP_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO7648249.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELVKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            26810..27295
                     /gene="sixA"
                     /locus_tag="ACHKXP_003301"
     CDS             26810..27295
                     /gene="sixA"
                     /locus_tag="ACHKXP_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO7648250.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         6981 a         6168 c         7659 g         6675 t
ORIGIN      
        1 gagtgaagta ataaacatgt taatacgatg gagtgaagga tgccgtgtaa ttctggttca
       61 agagtttttt atgccggaaa atcgcaggat tattctggat agtaaagaat cctggttaat
      121 aatctgtgat agtcagttgg gccatttaat gcgcagtatg tatcagggac gccgttttat
      181 tcagctgaat ctggaaaaat tgaaaggggt acatgatgtc gccttgccag tgaaatggga
      241 attcacacga agacagtgaa tagctttctg tatacgggaa tggcgaaaaa tggactgtat
      301 ggtgtgagtg tgaaacatct tgcgtgtgcg gagtgatgct tctcgttgct acagcggcaa
      361 tgataatgca gtgaaaaaag gggagcaata tgctccccca aaccgaaaga aaattacaat
      421 aatcaatgaa gttatttagt catcatcaga atgtcatgca aggcattttg tttcagtgat
      481 gccgatcgcg attttagcga attccatcat aaatcccctg atttttaagc ctgaagcagt
      541 caaaggaatt tctatgccct atatcgatat caccacgatg cgtgggatga tgccgcgcgt
      601 tgtgacatcc atgctgcccg agcattccgc tgtactggcg gaggactgcc atttccggtt
      661 tggtgttatt acaccagaac gtcagatatc cgggttagag aaaacattca caattaagcc
      721 aaaaacaatt tttcattacc gtgacgattt ctggtttgca tggccggatg tggtggatgt
      781 gatccgcagt ccgatcgctc aggaccccca cgggcgtatt tactacactg acgggcgttt
      841 tcctaaagtg acggatgcga ctattgccac aaaaggggac gggaatcacc cgacatcatc
      901 gtatcgtctg gggatccccg cgccgacgac agctcctgtc tgtactgttc agcagggcgg
      961 tgatgtttct gacgataacc cgaatgatga tgaaacccgg ttttatacgg aaacctttgt
     1021 ctcagattat ggtgaagaag gtccgccagg tccggcgtct ctggaggtaa cactccgtac
     1081 tccgggaact gcggtacaac tgacgctggc tccggtgcca ttgcagaatg ccagtattaa
     1141 acgtcgccgg atttatcgct ctgcatcagg tggaggggag gcggattttt tacttgtggc
     1201 tgaactggat gcatccgtgc tcagttacac ggacaaaata ccggcgaaaa accttgggcc
     1261 ttccctggcg acatgggatt acctgccgcc gccagagaat atgacaggcc tttgcctgat
     1321 ggctaacggt attgccgccg ggtttgccgg taatgaagtg atgttttcgg aagcgtatct
     1381 gccgtatgca tggccggaag tgaatcgtca cacgacggca gaagatattg tagctatctg
     1441 tccgctggga acgtcactgg tggtggcgac aaagggggag ccttatttgt tcagtggggt
     1501 atcaccgtcc acaatttctg gttccaaaat cccttcaatg caggcgtgtc tgagcaggcg
     1561 gagtatggtt gcgatggagg gctttgtgct gtatgcagga acaaatggcc tggtgtctgt
     1621 tgatgcaaac ggtaatgtcg cgctggcgac ggaacagatt gtttcaccgg aacagtggca
     1681 gagtcagttt aatccggcct ccattgtggc ttattcctgg cgtggtgagt acattgcctg
     1741 ttacacgaaa ccggatggta agcaggatgt gtttgtattc agtccggtga acatggatat
     1801 ccgttatctc agtacaccgt ttgactgcgc atgggttgat ctcgcgaaag atatgatgcg
     1861 cgtggtgaca ggagacaaaa tgtcagtgct tgccgggggc tctctgccct ccacgataag
     1921 gtggcattca aaaatttttt cattacctga aagaacctct ttttcctgta tcagggtgaa
     1981 atctccggcg cctgagcggg tggggatcac cattatggct gatgatgttc ctgtgattca
     2041 ttttgcgccg ggtacgttta agggaagtgt ggtgagactt ccggcagcaa ccgggcaaaa
     2101 ctggcaggtg atggtatccg gattcgggca ggtggaacga ataaccctga gtacatcgat
     2161 gtcggagatg ccggtatgac cagaaaaccg tggcgtgcgg ggaaggattt atccacagtt
     2221 gtggagaaca tggaaattgg caccgggcag cgtggtgacg gacgccacgc atttgtgacc
     2281 cgtgaggaac tggttggtct taaactcgcc cggcgtcgaa catcgggtgg tgcctcatat
     2341 gcactgaatc cgggtattga gattgacagt actttaatga ctgttgattt tcccacaaaa
     2401 ccgctgaatt ttaaggcggc cggtggattt ggctcggttc ttcttgaatg ggatatgcct
     2461 aattatcgcg gacattcact gactgaaatc tggcggggta cggaggatga ccttgctgat
     2521 gcagtgcttg ttgccacgac gccggggcag gtttacggcg atccggttga ccctggctgg
     2581 tcgggatttt actggatacg ttttgttaac gcggcaggag tgaaaggtcc atggaatgct
     2641 gaaaaaggca ctcaggcaca aacacagatc ggcgtgaagg ccatcattga ccagatccgc
     2701 gatgaggctg caaagtcgcc ggttgtgtcc gagctgcgta aagaaataaa aaacgcgcag
     2761 gggcaggctg taaaggatgc tgcaattaag acaaccgaag ttgtggggac tctcagggaa
     2821 gaaacgacaa gaacgattgg tggtattgaa acccgcatta gcacactgga ttcgtcaacc
     2881 agtgaatcgc ttaatgaggt cgacaagcgc atcactaaac tggataaaga aggcggtgag
     2941 gcgtttctgg caatgtggtc aaaaaaagcg ggagttgatg gtatcactgc ggggatcggg
     3001 attgtcgccg gaaaagacag tgaaggcagg cctgtaagtc aggttgcaat ttctgcgtcg
     3061 cagttgtttg tctttgaccc gaacaacccg gataacaccg cctatccgtt tgcggtatca
     3121 ggtggcaagg ttgtgatccc gaaagcgatg atttatgacg cggtgattga aacactggtg
     3181 tcgcggaagg ttgtggcgga tgaggtaaaa gccggggtaa gtatcacttc gccagttatc
     3241 cggagtgccg ttattcagaa cggaaacttt caggttgatt ctcagggtaa cctgaatatt
     3301 ggaggccttt tcagtgttac gtcacaaggg caactgacaa ttcgttactc taatcagaat
     3361 gtaggactgg tgatccgcaa tgataaaatt gaggtttatg atcagaatgg acgactggct
     3421 gttcgcatag gcagattacg ctgatcagga ggtgagtatt ggaatacggt tttgccattt
     3481 ataacagaaa taacgttaat gttacgggcg tgcttactcc catatttttc ctggacaggt
     3541 ttacggcgga gtcgggatca aaaacgtata cgaataaacc agaagggaaa acattgcagg
     3601 cagtctgttc attatttccg tggaataatg tatttaagga ccggaaagta ccgaagataa
     3661 ccattaatgg caatacggtg acgtggtcaa atcttgagca ggggatgggg tcatatattt
     3721 atacattctg ggggtgagtt gtatgtatgg tttaagcatt acgaaaccag atggcagctt
     3781 gtggataagc ccggggttta cgcctcagtg tctgatcaat aagggaacta ttccggcgac
     3841 tgagaaggct ttttttaaaa cgtcaattcc gtcaggaaaa agttgttttt tctttatcag
     3901 aacagagaat aaggccgatg tcatgtacac gcatgaacag attgatggat atcatgcctt
     3961 aaggcttcat caaattgtca ggggaacgaa tcctggtgtt acgactgttt atgcttttgc
     4021 gaatatggtt actcaacctt ctgagtatgg tatcgccatg tataacccgt cgggtgagat
     4081 gatttaccat ggcgaaatga tgctgcttga cgcgaagtta ataccagttg atatcaagtt
     4141 tgagaaggac cttggatatc catgcgcaat tatgcctgca ctggttggat attataactg
     4201 gaaacgaaca ccttatgacc gaccgattta caccacatcc actggtgcta caggaaataa
     4261 aatatattcc tgtgagcatt attccggtgg tgcaacatgg gatatccgaa agccgtatat
     4321 agataaagtt ctggttatta attcctcaat atatgattag cgtaagaaaa actttaatag
     4381 tgtaaaacga aagcgttcct ttcagacatc gcatccttta attcagagaa atacttaaaa
     4441 tgaaaagtat catgaaattt tttgcgtgtg cagttctggt gatgagttgc ttaactgcac
     4501 aggtaaatgc agcatcaggc gatagtacgg tatctctcgg gtttgcacac atacgcttcc
     4561 ccgggctgaa ggattttgtt agagacgcgg gtgtttataa ccgggatacg ttcaggaatg
     4621 ttgtaaacgt aaaccatttt aattcatcag gtgaatacga aaatgctatt gcccgggggc
     4681 atgacggcac agccaaaagc cccctgggga tgagcatcag gtatcgttac gagataactg
     4741 atgaactggg tgttatagcc tcttttacat gggcgcgctc catgactaat gcgcaggcat
     4801 ttattgatgt taagccttcc gatccatcac gagaggtaaa aaacccggca gcttcagcga
     4861 gaactgatat cagggctaat tactggagtc tactatccgg tccttcgtgg cggtttaacg
     4921 aatacctcag tgtgtatgca atggctggta tgggggttgc aaaagtcacc actgacttga
     4981 aaattaacga caatcttaat catggtgccg gtagtttttc tgaaagtaac agcaccaaaa
     5041 aaacgtctat tgcatggtct gtcggcgcgc aatttaactt caatgaaagt gttactatgg
     5101 acgtcactta tgaaagctca ggctctggcg actggaaaac tgatggttta tttgcaggca
     5161 ttggcctgaa attctgagta atacccgaca acatgtcata acccgctgtg atggcgggtt
     5221 ttttgttgcc cgtgcagggc aaaaatcgta gattatgcgc gggtgccttt cggctgatgg
     5281 ctggagggag agcctgaagg cctgatgtgg aaaggccccg agtcaaacat tttgtttaac
     5341 ccgaggccct aaccatcata ccttaagcaa gtagaaggtt agcgcctctc cactcaggag
     5401 gcaagcgcta tgtcgcaaaa atcgctcatc accgtcacaa tttgtatgac ggtaatcttc
     5461 accatctgga tgttacacgg ctcgttgtgt gagttccggc tgaatttgtg gggagcggag
     5521 tttgcggcgt tcttacagtg taagcagtag gaaaaccgcg acggggacga aagtccccgt
     5581 caactggttg ctgaggttca gccgatatgg cacccgtttc tggtgagaga atggacgaca
     5641 aaattctccg gtatatgcag cgtgttgtga gaaattcccg caaccctgaa tttatgaatg
     5701 aagttaaaga cgcctgcctt aaaaagcagg cgttttgctt tgaggcaccg gatggttttc
     5761 tggtgctgcg ttctgtgctc agtgacgacg gtatccctta tgttctggtg ttgctgggtg
     5821 tgtgtacggg gagtgccggt gttgagcgtt atctgccgga ggtgaagaca ttaaccagtc
     5881 tggctggcgg acgctgggct gaattccaca cggcaaggcg gggatttatc cggctgggaa
     5941 aacgtctggg ctttgagcga atgccggatg atgaggatgg cttcatggtg ttcaggatag
     6001 cggtctgact gccactgtat tcatcatcgt gtgtaaacca acattgcact tcacattctg
     6061 accctgcccc ggcagggttt tttgttatct gaggagccat aatgggcgga aaaaaaggcg
     6121 gtggtgatac caaagtaaaa ccaacagcag cgcaaatagc acaggaagag gtggcctgga
     6181 aagggtggca ggattacaaa aatattctcc gtccggcaga agataacttc atggaaaagg
     6241 tcgatgacct taacagtgag cagcagtacg acaatatcgc cggtacaaca aatctgggat
     6301 atcagaaaca gtttggtgaa acgcgaaggg agcttgcggg taatcttgct cagtccggcg
     6361 ttgacccatc cagtggtcgt tttaatgcgg taatggatgc aaatcaaagc gaccaggtga
     6421 ccgggcagat tgacacgacc acacgggggc aggtatcgca ggcagataaa tatattgccg
     6481 ggctacagga tgttgcggct ctcggttccg gtcagaaggc cgatgcgtta cagagtttta
     6541 actctctggc agacagcagt ctggcaaaag ccaaatcaga tgcacaggcg gcgtttacaa
     6601 aacaacaggg gcgggcctct cttgttggcg ctggtctggg ggcggttggg gcttatgcaa
     6661 tgcataaggc tggcggtagc ggaagtggag gttctaaaac gccagacgcc ggcgctaacg
     6721 ctattcagag tcaggctcag aactggagac tgtaattatg gagtatggca aatacgaaac
     6781 tctcgcaagg gctggttatt caggagcaga ccgcccacag ggtgactggc agacgtcagc
     6841 agcgctgaca cgccaacaat acgacgactg gcgaaccaga tatttgcccc gcgtggcaag
     6901 gctggctgac cttggtgaga acaacagcct gatgaatgca cagcttgccc gggtgggcgg
     6961 ccttgccact tccagtctcc gtacagcgca gatggcacag gataaccaga tggcgagata
     7021 cggggtaaat cgtccggata atcccaacag taacacactg ggtttgcgta atgctctggc
     7081 aattgctggc gcgaaaaatg gtatccgtga agcagagcag gatcgccaga tgaacatact
     7141 gacgggggct tctgcacctg caagacaaca actgagtgtt ggcggtcaaa tgatgacagc
     7201 gtaaggggga agatatgggg tacggcttac tggatattgc gaatcagtca cggcgtgagg
     7261 cattacaggg aataagtgac gcagaccgac gacgtgaaga aattgaggct gcgaacaaac
     7321 agatggcggc gcaacagaaa gcgcagaaca agcagaatat cggtacgggc attggtacgg
     7381 gggcggctat tggcgcatcc gttggtggtc ctgttggcgc tgttgctggt gcagtaattg
     7441 gcggcattgc tggttctttg ttttaaggag tggtgaatga gcggatttgc acaggggtta
     7501 cttgccggat tcagcactgt tgaccaggca atgacccgtc gtaaggagct tggtttgcga
     7561 gaagcacagc ttgctcagca acagaaaaat aacgagcgcg attttgaatt tgcgcagtct
     7621 cagtttgaac ataataaaga cgttgatcag cggaactttg attacagagc caaagttgat
     7681 gaccgcaatt acgcactgca ggaaagggag tttaacgcca accagaatta ccggaatgcg
     7741 tcgctgggga tggaactgca acgactccag ttgcagaaat acaaccagcg acggcttgag
     7801 tataacgata tgctggcacg tgatcagtcg gtgatggtgg cgctgggaaa agcgattgat
     7861 gcaggtgacc atgatgcggc ctctcgtctg ttcggcaaat tatcggatgc caaccccatt
     7921 aaaataatat caacggaagg ctatgctgcg aaggcgggtc aggccgtgaa caacctgcag
     7981 aaaatctttg atgataagcc ggacagggcg atcgattcgc tgaatacccc ggaaaatctc
     8041 gatgtgcttt ccggcgtgtt tgccccggaa ctacaacagc gtattggcat gcctgattca
     8101 accggggaaa aaacgataaa agaggccagg attggcagta tcgtaccggc gcagcaggaa
     8161 gggtacgtac ttattggcct tgatctcaca tacagcgatg gctccaccgc gcataaacct
     8221 gtaacagaat acggcagtgc gcaccctgat gatcaaaccg tgctggcgat acccgttgat
     8281 aaggctatcg ctcaggtcag ggatcgcagc aaatttgcag agatatcgaa aaattatggt
     8341 tattttatgc cgaagcagca gggactttct ctgaaagagc ttcagaaggg ggccagcaac
     8401 gtagcggcgg acgcgattaa gaatggcggt aatgctcagg ctgcggtgga tgaatattat
     8461 gctgcgactg gttcacaacc gcatcaacag aaaattcagc aacaaaaact tcagcaacag
     8521 gttatcaact gggcgggaga tgatcctgat aagctgtcat ttgccagaaa tgtagcggcc
     8581 cgtcagcctg aaatgctgga acctcagaat cagaaattgc tggagaacgg gtatgcgaat
     8641 tttctccgta ttcaaaaggc caggggggaa caggccagag atgaaagtgc ttcatctgca
     8701 tctcagttta tccgtggact gaaacagaat tacgcccagt aattcacgat attccattaa
     8761 taccatttcc tgatgcccgg ccattgtgcc gggttttttt atggagtctg tatggcctat
     8821 tcagaggaac agcgtcctga ggcgcaactc ggtaaccaga atcgtaacag cctgaacatt
     8881 cagcaacccg gcgaaactga cagctatgaa gcatttttct ctgattcaaa tcgctggaag
     8941 gataacagta cgtcgttcag cctgggcgat gtattgccaa caatgggtaa aggtttcgcc
     9001 cggtccgtcc ggggaacagg ggaaatggcc cgtggactcg gtgatgcgat gattcagagc
     9061 ccggtaaaaa caggggcgcg tattttaaat gagttcagcc gtatggggct gccgggtgtc
     9121 gcaactgtgc aggatatttt tgccggtggc agcagggggg ctgatgaggt catcgatacc
     9181 ctgcctgatg gcaaaaacgc ggttacagat actgtcggta aaggtctgaa ggtcaccggt
     9241 aaggctgtca gtgacggcgc taaatcctcg gatgaatggc tgaccagtaa gatgtctccg
     9301 ggtgctctgc gtgcgctgaa tacgccgatg accgaaggct atgatgattc tgcggtctgg
     9361 gtggcgaagg gcgtaaacct gattggtgcc cttgtacctg atatggttgc tggcggtgtg
     9421 gctaaaaagg tgggtgatgt cacattgcga aaaacgctga ccgccgggct ggagaaaaaa
     9481 tacatcgcgg cagggatgca gccggaaaga gccacggcac tggcagcaga agctgtcgat
     9541 aaaaaaatgc cggatttatt ccaggcgggc ctgatcaccc attccacagc cagtgcacag
     9601 gggcagagtg caatggcggc agcagatgct gttcttaatg cggattactc tgagctggcg
     9661 cagtcaccga aatttcagca gacgtttttg tccattgacg ccgatccgca gcacgcacag
     9721 cttactgatc gccagaaaat ggatctggca aaagagcgtg ttgccgatga ggtgcgcgcg
     9781 cagctggcaa cagatcctga attgctggct gtgaatgcca tggcggcaaa actgggcgat
     9841 gctcagttgc ttaatctggc gatgcggggc acggcgaata ccgttaaaag cggcattgtc
     9901 agaaatgcca cggaacaggg gacgattaat gcggcgcagg gtggctattc acgctatcag
     9961 gaaaacacgg cattgcgtga gaccgccgga atggatgtgt caccgtggga gggcgtggct
    10021 gacgcaacga tcgaaggtgc agcccttggt gctgcaatgg gggcaccatt cggtgcggct
    10081 gccggatatc gtggaaaacg tcaggcggca gatgaagcag ccatgcgtga ggctgaaagc
    10141 gttcctcagg acgaatcggc tccgcaaccc gaaccggttg atccggtggc acagcatcgt
    10201 gaatctatgc aggggatgaa tcgcgagcaa cttctggaac agtatgctga tgcggatatg
    10261 gcacatgagg gagacacgtc tgccgttcat cgccgggaag ccgccagcca gctgttgaat
    10321 gaactggatg aacaggcgaa gcgacaggcg gtgatggatg agctgaaggc gaagccgcgc
    10381 catgaactgc ttgaggaata ccgaaaactc agccagaagg aagggcgtac tgatactgaa
    10441 gagcagcagt tacaggcaat acgtgatgtg cttcgccctc agcgggaggc cagaccggag
    10501 gcacagccac agccggaaaa tgcggatgat ggcgacggga gtatttaccc gacggtgcgg
    10561 ttccgcgatc cggatgaagt tcgtattgaa attaacgaga gtggtgcgtc cagaccagca
    10621 gaacgcattg aaaaggtgcg tccggacaac cgttatttca cggatgaaaa aagtgctctg
    10681 gggagcgacg ttttccgcaa tgccagcgcc accggcctga aaccgtccgt agtgaagaaa
    10741 ggcgagaatc agtatgccgt tgaaatggat aatcctgcgt tctctgaaga tgtggcaacg
    10801 gagaccatta acactctggc tgacggagag cgtattactg atgctgatcc gatggaacag
    10861 cccgcgttca tgcgtgaccc gcgattccgt ggttttacgg gggatgatac agaagtgcag
    10921 gcccgtcttg ctcgtggcaa cgcgccgacg gcggaagaac tggtgcgttc ccagatggct
    10981 gaaggtgatg ccggtccgac agcacaggag ttaactgagc gtccacgcct tcccgctccc
    11041 ggcgatattc atcccggaca gggatatccg ttaccaggag aagtagcgcg tacgccggat
    11101 gaaaatcagg ccggacgtgg tggtcgtttt accacaaccg gtgaggtgaa gggccagagt
    11161 ttccagaaag gacgtgcgca ggcaccggaa aatgccgctg gtcgccaggg ggaaatactg
    11221 gagggcgaaa cagttcgtcg tggtctgccg tcaccggatg agcagaacgc cacagcacca
    11281 gtgcgtgaag gtcttcctgc acctgaaagt cagcgtgggg ttgatatgcc acagcctgaa
    11341 tcactccctc gtatggttcg tgactccctt ccggaactgg cacagcaggc agaagcacgc
    11401 agacagaccg gggataatcg ccagaccata accgatgttc cggatactga ggtgccggtg
    11461 ccggtagata aaccgagcac tcaccagcag gcgcgtggtg cgaaaattga agactttggc
    11521 gaggagatta agggcgcggc aaaacaccgt tatgcgcagc ttgctgaagc tatgggtaaa
    11581 acgctggagg atggggagta cgcgacacaa ccactgagca aactgtttcc aaagccggat
    11641 tatgcccgtc tggaaaaaga aggtgtggat agtgacacgc tggcaatgat ggcgttgtat
    11701 cgcagtgaaa ttcccaccag aacaagccgt aacatgcaga aatggatcag cattgttaaa
    11761 agcggtcgcg aagctactgc cggtatgctg gaggggaaaa ttccggcagc gaaactggct
    11821 gatatgatgg acagtaagcc ggggttacgg agcatgtcgg atacctggaa actgctccgt
    11881 acgctttctg cattgcagat tgataaagca tccggctatc gtgtcaggtc cggtgtttat
    11941 tcgtttgtcg ggggaaaacg ttacgatccg ccgcaaatga tgtattcgct tcgtgatagc
    12001 aaggggcgcg acctcttttt ctcggaaagc cgggatgagt tactgaaaaa ggcgaaagca
    12061 tatttcgatg aacaaggctc cagagaaagg gaaacgccag caacctctgc tgatgacaga
    12121 ataacctttg acgtgtaccg gcataaggcc agtggcgaca tttttatcgg ttatggcaaa
    12181 aacaggcaga aactgaaggg cggttttgag tctgcccgtg atgcacatga ttatgtgcgc
    12241 acacatcgtg atgagctggt taatcaggtg aaggcgctgc gtgaagtctc gcgtgaggag
    12301 cagcgtaacg ccaccaaccg cgatcgtacc ggaccagaac gccgcaaggg ggatgtttcg
    12361 ccggagcagt tcagtgatgc gtttggtttt cgtggtgtgc agtttggtaa ttatgtggaa
    12421 agtccgcgtc gtcaggctga tttgaaccgg gcttatgact cgctgcatga cctggctgac
    12481 gtgctgaacg tgccgacaaa agcgctttct ctgaacggtc gtcttgggct tgcttttggt
    12541 gcccgtggta agggtaaggc ggcggcacac tatgagccgg gtgaggtggc aatcaacctg
    12601 acaaaaggta acggaccggg ggcgctggcc cacgaatggt tccattcact ggataattat
    12661 tttggtcgtt atgatgtttc cactgacggg aagatcacgt caggtggcga ctatatgacg
    12721 gaagcacagc gtgccgggcg cgtatttaaa gacggcaggt atgttgatgc ggaatatccg
    12781 gtacgtcagg aggtttacga cgcgtttaaa ggcgtgatga aagccattaa cagcagtgac
    12841 atgttgcgtc gttctgaacg cctggatggc gttcgttcaa aaccgtactg gtcaacggat
    12901 gttgaaatgg cggcgcgagc ctttgagcgt tatgttcagg ataaagcgcg tatggctggc
    12961 gtggagaatg attatctggt caatatccgt aaggcggatg accatggaca gccggacacc
    13021 tacgcttatc cgacgaatgc agaactggat ggcggtattc gtgaggcatt cgatcacttg
    13081 ttccgaacac tgaaaacccg tgagacggac aagggagttg cgttttattc ccgtaagggc
    13141 gtgacccgca cacctgaagg caatctgatt tcggatgtta accgcagtgc ggaagccaaa
    13201 ggcagtccgg tcccgcaggt tgaagcggtt gcccgtggcg tgatgagcgg cattaaggac
    13261 agtgacctga aggtccgtgt ggtgaagtca cagaaagagg ctgaagcgct ggcgggtgaa
    13321 ttgttcgatg gttacggcag ggtgcacgca ttctatcgtc cggataaacg agaaattgtc
    13381 ctggtggcgg ataacatccc tgacgggcgg accgttcgcg agaagctgcg tcacgagatc
    13441 attcaccatg ccatggagca tgttgtcaca ccagcggaat atcagacgat tatcaaaacc
    13501 gtgctgaaaa cccgcgacag tgataacgtc accatccgtg aagcctggcg taaggttgat
    13561 gcttcctatg gtaaggaatc accggaagta caggcgggtg aatttctggc acatatggcg
    13621 gagaaacagc cgaataaatt cgtggcggca tgggagcgtg ttgttgccct ggtcaaaggg
    13681 gtactgcgtc gtacggggtt actgaagccg acggaactga acgatatcag acttgttcgc
    13741 gaaactattc gcacgttagg cctgcgtgtg cgggaaggtt acacgccgcg tgaggatggc
    13801 gcgggcgcat cgtctcagta ctcccgtagt ggtaaacctg atccgttcaa agtgccggaa
    13861 ggtgagggcg agcgttatcg tgatgacctt gccagaatga tgaagtcgtt acgttctggt
    13921 gcaatgactg caaacatcgg gcgtacgccg ccggtattgc gccaccttgg cgcaccagat
    13981 ttgccgctgg ttatctcccg cgacactgtg cggaaggcaa ccaatggagt gaaacatgtg
    14041 gtgccaatgg atgttatcga gagacttcct gagctgatgc acgatccgga tgcaatttac
    14101 cgttccgcga cagaaagaaa tgcggttgtg atgctgcttg atgccgtgga taaaaatggt
    14161 gatccggtgg tgtcggcagt gcacatgaag gcaacagata aacgaataga aattaacaag
    14221 gtggcttctg tatatggaac aaaagggggg atgaacaaag ctaatagcct ggataaagca
    14281 ggtttgacgc tttaccggaa ggaaaaatta agccgcgata accctcagta cagtgggctt
    14341 caattgccca aagaggagcg ttcttatcgc ggctctgtag ataaaatact ctatcctgaa
    14401 gatattcgca atgggccgta ttactcccgt accagcagtc tgacaccgga agagacaatt
    14461 gcatcgcgtt ttgtgcgtca gatgcaggat aaattccagg tgctgaaagc tgttcaggag
    14521 aacatccgta aaactggcgg aaaaatagac gacagtaaca acgcttatat ggcggaagaa
    14581 ctcttccacg ggaaggcgga aaacgacctg aacgtgatga aggagcgcta cgttcagcca
    14641 ctggctaaat tactggcgga ctacaaaatt gcgcaggccg atctggatga gtacctctac
    14701 gcccgtcacg cgccggaacg taacgcgcat atcgcgaaaa tcaacccgaa aatgccggac
    14761 ggcggttcgg ggatgaccaa cgcggaagcg gcggaaatca tgcagcgtgt acgtaacagt
    14821 ggcaaacagg cacagtatga ccgtctggca gggattattg acgatatgct ggcccgtcgc
    14881 cgtgagctta tccgtcaggc cggacttgaa gagaacggtg tggtggatgc ctggcagaac
    14941 gcctaccgtt actacgttcc cctgaaaggt caggatgttg acggtgtggt gtcactgccc
    15001 cgtacaggta agggcttcac catcggcgga cgtgaaagca ggcaggccat ggggcgtgca
    15061 tcccgggcac agtctccgtc cactcaggcg atacaggacc tgagcgaatc gctgatccgc
    15121 catcgcaaaa acgaagtggg taacgccttc ctgaaactgg tgcaggataa tcccgacaag
    15181 gattactggc aggtattcac cgatgacaga ccggatacca tgcggacgat tgcagagcgc
    15241 aaggaccagg aaactggtga aaccattcgc gaagctgtcg aacgccctgt accgatggca
    15301 atgatggcag accggtactt caccaccaaa aagaacggca aaacgtacta catcaaactc
    15361 catgatccgc gcctgatgcg tgcgatgaag aatatgggac cggaaaccag taacgccgta
    15421 atccgcacgc tggggaaagt taaccgcttc ctggcaacgg tgaacacgtc gtataacccg
    15481 gaattcctgg tcagtaactt catccgtgac gtgcagacgg cggtgatgaa cctgaaggcg
    15541 gagcagggaa ggagcgacgg taaactgaaa gggctggata acttatccgc cctggctgtg
    15601 gtgaaagaca gccgttctgc catgtcagcc gtatacgcca gtctgcgtgg taaaaccctc
    15661 acgggaaaag gtgcacagtg gcagaaggtg tggaaagagt ttgttgagga cggagggaaa
    15721 accggctggt ttaacatggg tgaccttgaa ggccagcaga aggaaatgga tcgccttgtc
    15781 tcactggcga aggggggatg gaaaggccag agtatcggtg catggaattc gttccttaac
    15841 cttgtcgagg atgccaacgg tgcggttgaa aacgctctgc gtctttctgc ctataagcac
    15901 gcccgtgata ccggtttgtc acgccagcag gcggcgtctc ttgccaaaaa catgacggtg
    15961 aactttaacc gtcgtggtga gcagggggcg ctgatgaatt cgctgtacat gttcgccaac
    16021 gccagcattc agggaacggc aaatctggtg agaacgctcg gacatcttaa tggtgaggga
    16081 cctttactgg agcgccttcg ctggaagaat ctgaatgttc cgcagaaaat cgcgcttgcc
    16141 gctgtgggag caggttatct gcttggctcg cttaaccgca gcgtggcggg tgaggatgat
    16201 gacggggtta actggtatga caaggtgccg tctcatgtga aagagcgtaa cctcgtcatt
    16261 atgaaatcgg tgttcggggg caaggccgga gagtactgga gtattcctct gccttacggg
    16321 tacaacgttt tcttcctgct cgggcatact gctgaaggtg tggcggcggg tgacctgacg
    16381 gcgtcccgtg ctgccggtaa tgttgttggt ggtgtgcttg gtgcattcag cccgattggc
    16441 agtgagacgt cggaaacact gtccggggca ttgctgaaaa atgcagcgcc gaccattctg
    16501 cgtccgtttg cgaaccttgc catgaatgaa aacttcatgg gggcgcagat ttaccaggag
    16561 aacatgccgt ttggtacacc aaaacctgac agccagctgg gaagacgttc aacgccagaa
    16621 gcgtacaagg cgtttgcatc ctggctgaat gcgttctctg gtggcagcca gtaccgtccc
    16681 ggcgcggtgg atatcacacc ggaatcgctg aaatactggg tggactatat ctccggaggg
    16741 acagggcgct tcatttccaa aaccacggat gcggcggtga aatcgctgaa tggtattgat
    16801 ataccggagc agcaggtgcc cttcctgggg aaaatttcag gtgaggtgat gccgtatgct
    16861 gaccagcaga agatgtacga ccggatgaca gaggttgcgc agtatcacgc agagctgaag
    16921 agtctgaccg gtgcagaaag aacggcgttc attgacgaga acaacggaaa attgtcgatg
    16981 aacgggctta tgcaggatac ccggaagaga ctgaaggatt tgcgtaaaca gcgtgatgcc
    17041 atttacgccg acagtactct cagtctggcg caacagtcgg cgatggtgaa atcggttgag
    17101 cgggatatga aaattgccgt ggatcggttt aaccgcgagt acaacaaaaa agtgggagtg
    17161 gagtaaagaa catgccccgt acggaagtgc ggggcatgct taacgggatg tgtacataaa
    17221 tgggtactga tatgacgatt atttcggtgc tttctgtatt taaattaatg agttaatact
    17281 tgtattcatt atattaattt aactaagagg acaatgcggc atgagtatac ccgctaatgg
    17341 agtgcggggt aagtacgctg ccgctcgatt gcttaaaccc tcgccattta tgccgggttt
    17401 ttataatttt tcttaatgtt ttccgcacgt tctgcttttt ggcgtgcttc tgctttacgc
    17461 ttattgctca tgtcgttacg aatctgtgca tgactcatta acgcgaagat aaaggtgccg
    17521 ccgcagatgt tccccgctaa agtaggtagt gcgaagggcc agatgaaatc gctccagtgc
    17581 agcgtaccgt taaacaccag atagaggatt tcaacagaac cgaccacgat atgggtggtg
    17641 tcacccaggg caataagcca ggtcatcaat ataatcacca caatctttgc cgcacccgct
    17701 gcaggaaaca tccaaaccat agtggcgatc agccagccgg aaatgatcgc gttggcaaac
    17761 atctcgctgg gggtgttctt catcacatcc atgccgattt tgacaaatgc atcgcgagtt
    17821 tcttcattga agataggcat atattcaaat gcccacgccg caatacctgt cccgagaata
    17881 ttacccagca gcacgacgcc ccataaccgt ataagtaagc cgacgttgct cattgtcggt
    17941 ttttgcatga cgggtagtac cgcagtcacg gtattttcgg taaataattg ctggcgggcc
    18001 ataatgacga taataaaacc aaaggtataa ccgagattct ccagcaagaa gctgcccggc
    18061 acaccttcca gttcgacatg aaatatccct tttgccagta acgaagcgcc catcgacaga
    18121 cccgccgcaa tggctgacca cagtagcgcc attgcgtcgc gttccagctc tttttcacca
    18181 tcctggcgga tatgctcatg aattgccatc gcccgggagg ggagtcggtc ttcatctatt
    18241 tctatttttt tgccgcgctc tttttcttcg ctctcaactt caatttcgtc gctgtgttga
    18301 tcaattttgt cgttgtccat ggtctcttct cttgaattag cacgtatagc taaagcgtag
    18361 cggctttttt gctcgcaact gacgggagtt actctgaaaa tgtagaaaag gctgcgtttg
    18421 cctttttctg tttctataaa atcaagtagc ctacagggcg gcgattacca ggctatgatc
    18481 aaatcagcaa atcagggcgt ctggacatca gttgacgtgc tgttacaatc gcccacacct
    18541 aaacaggcgg atacggtatc gttccgtcat ggatggcaaa ctgcataagc cataaaaaaa
    18601 cagggagaca tttatgaagc ttcgcctgtc ggcgcttgct ctgggaacta cgcttctggt
    18661 ggggtgtgcg agttccggta cagatcagca agggcgttct gacccgttag aagggttcaa
    18721 ccgcaccatg tacaacttca acttcaatgt attagacccg tatattgttc gaccggtcgc
    18781 tgtcgcctgg cgtgattatg ttccgcaacc ggcgcgtaac ggtttgagca actttactgg
    18841 caaccttgaa gaacctgcgg tgatggttaa ctacttcttg cagggcgacc cttatcaggg
    18901 gatggtccac tttacccgct ttttcctgaa caccattttg gggatgggcg gttttattga
    18961 tgttgcaggg atggcgaacc cgaaactgca acggactgaa cctcaccgct tcggtagtac
    19021 gcttggtcat tatggcgtgg gttatgggcc ttacgtccag ttaccgttct acggtagctt
    19081 cacgctgcgt gatgacggtg gtgatatggc ggatggtctt tacccggttc tttcctggct
    19141 gacctggccg atgtctgtgg gtaaatggac gcttgaaggg atagaaactc gtgcgcagtt
    19201 gttggattcc gatggtctgc tgcgtcagtc gtccgatcct tatattatgg tgcgcgaagc
    19261 gtacttccag cgtcatgatt tcatcgctaa tggcggcgaa ctcaaaccgc aggaaaatcc
    19321 gaacgcacaa gcgattcagg atgatttaaa agatattgat tctgaataag aaacaaataa
    19381 aaaaggtgag tcgcaaaact cacctttttt gttgttatcc ctcaattacc acaagtaaaa
    19441 ggagataaac cgacaatgac ccggacgtaa tgatgtaaaa atactgatgt tcgtcactga
    19501 ctatttccga tattcaggcg ttgaaaaatc aaaaaactgg aaaaataatt ttaaatatca
    19561 tcaaaattaa cacctggcgc agatagttga ttaatttcat tttgatattt cgcctggcga
    19621 ttttgaagtc ttttttcaac gttatcttcg agatccttaa agtctttgta tttattttca
    19681 ggatggtcaa cgtcaacttt gttagcgaat aaattagaaa ttatttcttt tacattatcg
    19741 cgtctaaatt ttgcatcgtc cacttgaaaa tcatatctta ttgctcgacc ataacgttta
    19801 aaaacgtcgt ttggttctct cagtctttcc ccgttgagct cgtttacaac ctgatgatcg
    19861 gaaccagttg caaatgattc tgccatgata gctttactgc tttcaaacca cgctgaagca
    19921 ctcctggcgt tttcactaca atattgggtg agactatcac cattaatacg aattcctctt
    19981 tgaatgtggt agtcgtcctg atcgtagtaa ccgagatata agtaatcatt tccgttttta
    20041 tttttaatta caatagcgag agagtcgcct tttttatctt taccgtacca ggtaaataag
    20101 tccgttggtt cgcttttccc gctctcatca aattccgtta ccgtatgtct gagtttgtgt
    20161 gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa tgcgctgaag
    20221 atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt aatgatagta
    20281 cggacgagtt ctttgtcttt atagttcgca ggtaatttta ttggttcttg gttatcgata
    20341 ctaatagtaa tatggtctgt atcattggag ccaatatcga tataagtaac tgcggaatta
    20401 ttgatattaa ttctctcagg attgctgttt cctcgtatgg cattttttat aagctcaagt
    20461 agatgtttaa tacaggtgct tattccttcc ttaccattaa aaatggtttt tgaactcttg
    20521 tactaatcca ttaacttcat cattgttatt ttcattgatg gattctgtgt aagcagcgct
    20581 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
    20641 aaatatatat tacctaataa aatagactat caaaaaacgc tcatttattt agaacctatc
    20701 tttaatttgc gtgtatgagc gcacaatatc cagggggggt gagtatcctg gaaattcaga
    20761 aaagtaaact ctttgagaag gacgagcaga acgcctgaaa aaatgcgttc cccttcaggt
    20821 ttgtctgtgg ataccactga ttgatttgaa agggtaagct gacgtcaggc tgtttgcaaa
    20881 actcaggagg taagcaatga ggacactact ttcggtgaag tgggggagtt aaagtcacct
    20941 gctatgcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
    21001 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
    21061 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
    21121 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctgt
    21181 gcaggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
    21241 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
    21301 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
    21361 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
    21421 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
    21481 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
    21541 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
    21601 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
    21661 ttactgtcaa taccgttggc ggtagctgcc aatgcttgcc cttgctgagt ttggccagca
    21721 ggagattgca taatttggcc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
    21781 ttcgcgcgag cgtagacggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
    21841 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcgg ttgtaccccc gacagagccg
    21901 cctgcataag tatcgttaaa ctctgtagcc agaccatagt tagaggtaat agaagcgccc
    21961 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
    22021 gcgatgttat cggctttcag gctacgacca gatggagacg ttccgctgat atttacatcc
    22081 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatagt aatcaatgcg
    22141 gggttacggc tgacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
    22201 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
    22261 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
    22321 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
    22381 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
    22441 ctggaatgtt gcaagtatgt ttctatcttt tttcaaatat gatctaagaa acgcgatcca
    22501 gctggcaaaa tttggaggtg gcgcactcat ggattcgggt gaattttgtt ttagatcatt
    22561 tttgagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
    22621 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
    22681 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
    22741 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
    22801 agaagcagag caaacgctgg cggcgctgac tgaaaaagcc cgtagcgtgg aatctgaacc
    22861 ttgcaaaatc accccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
    22921 cacttttgcc tgcgaagctg aaatgctgat tttccagctg ggtctgcgtt aattcctctc
    22981 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctctt cttcttttct
    23041 ctctgtgtca tagctcccac tttccctttt ctccacttgg ctaaatgtaa aaaaatggtt
    23101 aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca
    23161 ggttttaccg ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac
    23221 gccttttgcc cgtcaggcga cggcttttca tggcattccc gcggttgatt tagggaagat
    23281 ggtggtaggc gaactgctgg cacgcagcga gatccccgcc gaagtgattg aacaactggt
    23341 ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg
    23401 tacgggaatg aatgtacata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt
    23461 ccaggcagtt gcaaacgtcg cagaaagcct gatggcggga actattcgag cggggattgc
    23521 cggtggggca gattcctctt cggtattgcc aattggcgtc agtaaaaaac tggcgcgcgt
    23581 gctggttgat gtcaacaaag ctcgtaccat gagccagcga ctgaaactct tctctcgcct
    23641 gcgtttgcgc gacttaatgc ccgtaccacc tgcggtagca gaatattcta ccggcttgcg
    23701 gatgggcgac accgcagagc aaatggcgaa aacctacggc atcacccgag aacagcaaga
    23761 tgcattagcg caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa
    23821 agaagaggtg atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa
    23881 tattcgcggt aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa
    23941 acacggaacg gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcggtgat
    24001 cctgatgact gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag
    24061 ctacgcattt actgcgattg atgtctggca ggacatgttg ctcggtccag cctggtcaac
    24121 accgctggcg ctggagcgtg ccggtttgac gatgagcgat ctgacattga tcgatatgca
    24181 cgaagccttt gcagctcaga cgctggcgaa tattcagttg ctgggtagtg aacgttttgc
    24241 tcgtgaagca ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt
    24301 gcttggcggt tcgattgctt acgggcatcc cttcgcggcg accggcgcgc ggatgattac
    24361 ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc
    24421 tgccggtggg cttggcgcgg caatggttct ggaggcggaa taatggaaat gacatcagcg
    24481 tttaccctta atgttcgtct ggacaacatt gccgttatca ccatcgacgt accgggtgag
    24541 aaaatgaata ccctgaaggc ggagtttgcc tcgcaggtgc gcgccattat taagcaactc
    24601 cgtgaaaaca aagagttgcg cggcgtggtg tttgtctccg ctaaaccgga caactttatc
    24661 gccggagcag acatcaacat gatcggcaac tgcaaaacgg cgcaagaagc ggaagctctg
    24721 gcgcggcagg gccaacagtt gatggcggag attcatgctt tgcccattcc ggttattgcc
    24781 gctattcatg gcgcttgcct gggcggtggg ctggagctgg cgctggcgtg ccacggtcgc
    24841 gtttgtactg acgatcctaa aacggtgctc ggtttgcctg aagtacaact tggattgtta
    24901 cccggttcag gcggcaccca gcgtttaccg cgtctcatag gcgtcagcac agcattagag
    24961 atgatcctca ccggaaaaca acttcgggcg aaacaggcgt taaagctggg gctggtggat
    25021 gacgtcgttc cgcactccat tttgctggaa gccgctgttg agctggtaaa gcaggatcgt
    25081 ccatcttccc gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg
    25141 ttgctgttca aaatggtcgg caagaaaaca gaacacaaaa ctcaaggtaa ctatccggcg
    25201 acagaacgca tcctggaggt tgtcgaaacg ggattagcgc agggcaccag cagtggctat
    25261 gacgctgaag cacgggcgtt tggcgaactg gcgatgacgc cgcaatcgca ggctctgcgt
    25321 agtatctttt ttgccagtac ggacgtgaag aaagatcccg gcagtgatgc gccgcctgcg
    25381 ccattaaaca gcgtggggat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc
    25441 actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat
    25501 catgcgctga agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa
    25561 gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt
    25621 gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg
    25681 gtggcggaag tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa cacgtcatct
    25741 ttaccgattg gtgatatcgc cgctcacgcc gcgcgacctg agcaagttat tggcctgcat
    25801 ttcttcagtc cggtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg
    25861 gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac tcccattgtc
    25921 gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct
    25981 atccgcatgc tgaccgaagg tgaacgggta gagcacattg atgctgcgct agtgaaattt
    26041 ggttttccgg taggcccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa
    26101 attattcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt
    26161 tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat
    26221 ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcctg ccatttaccc gctcattggc
    26281 gcacaagggc aggggcgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg
    26341 ctgaatgaag cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat
    26401 attggtgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc
    26461 gattctctcg gcgcgggcga agtggttgca ataatgcaac gactggccac gcagtatggt
    26521 tcccgtttta ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg
    26581 aaaacaactg caactgacct gcaataagaa ggtcaaagct atatgaatcc gcgctgaatg
    26641 gcggagtgtt ggtcaaaatg taaacgcata ttgactatac ttacgccatt gaggtaaaaa
    26701 acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg
    26761 tttcagatga aagaaaatgg gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt
    26821 tatcatgcgt cacggcgacg cagccctcga tgccgccagt gattcggttc gtcctctgac
    26881 cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg ctaaaaggtc aaaaagtgga
    26941 aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg
    27001 ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga
    27061 tgttggtctg gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact
    27121 ggtgatctct cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac
    27181 gccgcctatg tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg
    27241 cacattcaac tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgagt
    27301 tccaaattat gcccaatgag gtagattcat tgggcataat tgctcatcag gcagcggata
    27361 tctgtcaacc tcactactat cgaagactct ccccatatat aaataaaaca ctttctaatt
    27421 tcattcggaa attatttttc tgcgcgtatc ttgattttgc ccaaagtaac atgttgaaat
    27481 gaa
//
LOCUS       DAZFZC010000071        27332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFZC010000071 DAZFZC010000000
VERSION     DAZFZC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.071"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(82..363)
                     /gene="yehE"
                     /locus_tag="ACHKXP_003302"
     CDS             complement(82..363)
                     /gene="yehE"
                     /locus_tag="ACHKXP_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO7648251.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(626..1735)
                     /gene="apbC"
                     /locus_tag="ACHKXP_003303"
     CDS             complement(626..1735)
                     /gene="apbC"
                     /locus_tag="ACHKXP_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO7648252.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1867..3900
                     /gene="metG"
                     /locus_tag="ACHKXP_003304"
     CDS             1867..3900
                     /gene="metG"
                     /locus_tag="ACHKXP_003304"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO7648253.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLANELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4041..7835
                     /locus_tag="ACHKXP_003305"
     CDS             4041..7835
                     /locus_tag="ACHKXP_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO7648254.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYSMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARSWLLKYPEHAITGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDNALLHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDASGSRLKDLPKPNKSDDESRADEAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPMVRHLTRRLIWGVYSADNQLQACFRVAEDNSYST
                     ADDDLFTLPEGDISLGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEVE
                     RNASELIRWAGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAERYGWGSNSTQEAQFSVIDAITASELINDIEAL
                     FE"
     gene            7845..11477
                     /gene="yehI"
                     /locus_tag="ACHKXP_003306"
     CDS             7845..11477
                     /gene="yehI"
                     /locus_tag="ACHKXP_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO7648255.1"
                     /translation="MDKELPWLVDNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPAQTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMQWLSTQARAVLNSYLSAPPKPVIDSTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATESARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     VFVKPCDNSEAALFALRLLYEQGHSELLQTVANRWDRADMWPALEKILTQNPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTNDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYNLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11538..11855
                     /gene="yehK"
                     /locus_tag="ACHKXP_003307"
     CDS             11538..11855
                     /gene="yehK"
                     /locus_tag="ACHKXP_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO7648256.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            12162..13250
                     /gene="yehL"
                     /locus_tag="ACHKXP_003308"
     CDS             12162..13250
                     /gene="yehL"
                     /locus_tag="ACHKXP_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO7648257.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVECIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13261..15540
                     /gene="yehM"
                     /locus_tag="ACHKXP_003309"
     CDS             13261..15540
                     /gene="yehM"
                     /locus_tag="ACHKXP_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO7648258.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQKIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCATIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     EADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            15533..16669
                     /gene="yehP"
                     /locus_tag="ACHKXP_003310"
     CDS             15533..16669
                     /gene="yehP"
                     /locus_tag="ACHKXP_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7648259.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVCQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIQSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16666..18666
                     /gene="yehQ"
                     /locus_tag="ACHKXP_003311"
     CDS             16666..18666
                     /gene="yehQ"
                     /locus_tag="ACHKXP_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO7648260.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQTLWLGGISQPLIHYEAAFNRALQAAETCNWRWVSESLRQLRASVDA
                     FHARASHYNAGECLHQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLGQFRNTESEARVEAINNGVLLCEQLFPMLQQQG"
     gene            18791..19253
                     /gene="yehR"
                     /locus_tag="ACHKXP_003312"
                     /pseudo
     CDS             18791..19253
                     /gene="yehR"
                     /locus_tag="ACHKXP_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
     gene            complement(19294..19764)
                     /gene="yehS"
                     /locus_tag="ACHKXP_003313"
     CDS             complement(19294..19764)
                     /gene="yehS"
                     /locus_tag="ACHKXP_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO7648261.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(19811..20530)
                     /gene="btsR"
                     /locus_tag="ACHKXP_003314"
                     /gene_synonym="yehT"
     CDS             complement(19811..20530)
                     /gene="btsR"
                     /locus_tag="ACHKXP_003314"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO7648262.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(20527..22212)
                     /gene="btsS"
                     /locus_tag="ACHKXP_003315"
     CDS             complement(20527..22212)
                     /gene="btsS"
                     /locus_tag="ACHKXP_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO7648263.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            22434..22499
                     /locus_tag="ACHKXP_003316"
                     /pseudo
     CDS             22434..22499
                     /locus_tag="ACHKXP_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22727..22975
                     /locus_tag="ACHKXP_003317"
     CDS             22727..22975
                     /locus_tag="ACHKXP_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7648264.1"
                     /translation="MRVESCIAKEKISKMPKGSVPALQQEMLRRIRKRYDNAEIIIKS
                     ASNDGLTILRATDKESAHKFVQETLQEARETADDWFIH"
     gene            23180..24394
                     /locus_tag="ACHKXP_003318"
     CDS             23180..24394
                     /locus_tag="ACHKXP_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
                     /protein_id="HFO7648265.1"
                     /translation="MFAAKPELSQRCISGLLTTGPNDENVNNKPSSDMPNRIKPVVSD
                     SQIGIYTTDSNTLKDGLCSQAHIGTQPYRPGADSPITVRSFLQITLSNNNYKSLEHLQ
                     TRAENYLRYRKAEENILRSTVEGLTNPESPVFKQTAWMGHLERGLWKTETRWDGNDRE
                     QLGKEALGSEEPKPGSPFYGSRGLKLSDSAHSAFSMMLCGSEGPFTKEQALSGFELAQ
                     TGQVLAGRLKIQERVKFRADNRIDAQRNGTHSTRTPTGMDLSQDIGTIMRDKAGLPVM
                     SGTSGSSSDATLATRYAAEHFGKTWAAPGLSQAEGCKAISDLSHHYFRAEGSSPPQSM
                     AMATGINKVRYDAGMEEKYVNTLDIFTHSYPEIYAGVALTIAGAGGNDEQAMYNVTQE
                     AARILHEAETKD"
     gene            25046..26287
                     /gene="nleA"
                     /locus_tag="ACHKXP_003319"
     CDS             25046..26287
                     /gene="nleA"
                     /locus_tag="ACHKXP_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO7648266.1"
                     /translation="MNIQPTIQSGITTQHNQHHHTEQTPPTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDAYIAPHLEEGSAGKKALDMFGLFNV
                     TQMALEIPSTVPGISGKYGVQMNIVKPDIHPTTGNYFLQLFPLHDEVGINFGALPGPL
                     QKFLSQNPNPWFGVTAQVVRNHGIELPIVKTDNGWKLVGETPLTPEGPKANYTEEWVI
                     RPGELDLKYGSTPFQSTLGLEFGAHFKWDLDNPNTKYAVLTNALANAAGAAGGYTLSQ
                     YTGTNAMLSPHIGSMAGQALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAEFGVAR
                     FTDYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNG
                     SPHHNPSNYVDTFDIIQETRV"
     gene            complement(26415..26537)
                     /locus_tag="ACHKXP_003320"
                     /pseudo
     CDS             complement(26415..26537)
                     /locus_tag="ACHKXP_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(26585..27173)
                     /locus_tag="ACHKXP_003321"
                     /pseudo
     CDS             complement(26585..27173)
                     /locus_tag="ACHKXP_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7244 a         6868 c         6592 g         6628 t
ORIGIN      
        1 tgttgatatc aataaaaaag ccgaaatcat ttatataaat gatttcggct ttctaaatgc
       61 aattaaaatc aggttgccaa attaatcgta gcgatttaac acgattcgac gtttgctatc
      121 gctacctgca acaaccactt ctgtcgttac acctttgacg ggcgaagaaa acagatgacg
      181 ggtgtcaaca ttatacgtta actgaccaca atgctgttga ggttgaccat taaccatagc
      241 catactgcat gttgggggac tgatcctgcc attaatcgct aatgtggcag tctctgaaga
      301 gaacgcggga gaagccaatc cataggcaag aaaaataatt cctgacagcc aatacttatt
      361 cattgaacgt tatccctgta gtaaaggtta tgcctggata gaatgagtgc ataacaaact
      421 atagctgtac atccactaca cagccacgaa ggatgataat gaagcattgc ctgtacgatc
      481 aatcgacttt gtagagtttc ggacgaaggt ccgcagaata ttcgcagtat taaataagtg
      541 ttcattaatg cggcaaaaac aaatacaccg catcaatata acatctctgc ggtgtatgaa
      601 gaataaccag aatggttaat aggcgttaga ccgcgcggaa ggaaatctcg cctggaatga
      661 cttcaccctg ccagtagagc tgagctgcaa cgcggtcagc cagctggcgg tagatagcgg
      721 taaattcgct ctccggacgg ctgataaccg ttggcgtgcc tttatcgaga tcctcacgta
      781 aggagatgtg taacggcatc tgccccagca gctgtgtgtg gtatttttca gccagtttct
      841 cagcaccacc ggtaccgaaa atcggttcat ggtgaccaca gttactgcaa atatgcacac
      901 tcatgttctc gacaatacca agcaccggaa cctcgacttt ctcgaacatc acaatgcctt
      961 ttttcgcatc gatcaacgcg atgtcctgcg gggtggtgac cacgactgca ccagttactg
     1021 gaatgttttg cgccagcgtc aattgaatgt caccggtgcc tggcggcata tcaagcacca
     1081 gatagtcgag atccggccac aaggtttcct gcaacatctg cattaacgcc ttgctcgcca
     1141 tcggtccacg ccacaccatc gcattgtcgt cagtgaccag ataaccaatt gaattggttg
     1201 ccaggccatg agacataatc ggtgccatat gggtaccgtc cggcgaagtt ggacgctgat
     1261 tttctgcgcc cagcatggtg ggaattgatg ggccgtagat atcggcgtcc agaatgccga
     1321 ctttcgcccc ttccgccgcc agtgccagcg ccaggtttac cgccgtggag gatttaccca
     1381 cgccgccttt accggaactg accgcaatga tgttcttcac accattaata cccggctgat
     1441 tcttcacgcg ttttaaggtg gcgatattgt gcgacagttt ccagtcgata gctttcgccc
     1501 cggtgatacg cagcagttcg gcgctacatt gctcttttaa ctcttcaaac gcgctatgcc
     1561 agacgaatgg cataaccagt tccacatgca acgtgtcgtc catccaggcg acatggtgca
     1621 acgctttcag cgtggtgagg ttatgcttca gggttgggtg ctgaaaattg gccagtgtcc
     1681 cggcgaccat tgcgcgcaag gcttctggtg atttggcctg ggattgttcg ttcatcccga
     1741 ctcctttttt gtataaataa accatcagct gatagtttac ctgaagaata tagagaagta
     1801 cttacttaac attttcccat ttggtactat ctaacccctt ttcactatta agaagtaatg
     1861 cctactatga ctcaagtcgc gaagaaaatt ctggtgacgt gcgcactgcc gtacgctaac
     1921 ggctcaatcc acctcggcca tatgctggag cacatccagg ctgatgtctg ggtccgttac
     1981 cagcgaatgc gcggccacga ggtcaacttc atctgcgccg acgatgccca cggtacaccg
     2041 atcatgctga aagctcagca gcttggtatc accccggagc agatgattgg cgaaatgagt
     2101 caggagcatc agactgattt cgcaggcttt aacatcagct atgacaacta tcactcgacg
     2161 cacagcgaag agaaccgcca gttgtcagaa cttatctact ctcgcctgaa agaaaacggt
     2221 tttattaaaa accgcaccat ctctcagctg tacgatccgg aaaaaggcat gttcctgccg
     2281 gaccgttttg tgaaaggcac ctgcccgaaa tgtaaatccc cggatcaata cggcgataac
     2341 tgcgaagtct gcggcgcgac ctacagccca actgaactga tcgagccgaa atcggtggtt
     2401 tctggcgcta cgccggtaat gcgtgattct gaacacttct tctttgatct gccctctttc
     2461 agcgaaatgt tgcaggcatg gacccgcagc ggtgcgttgc aggagcaggt ggcaaacaaa
     2521 atgcaggagt ggtttgaatc tggcctgcaa cagtgggata tctcccgcga cgcgccgtac
     2581 ttcggttttg aaattccgaa cgcgccgggt aaatatttct acgtctggct ggatgcgccg
     2641 attggctata tgggttcttt caagaatctg tgcgacaagc gcggcgacag cgtaagcttc
     2701 gatgaatact ggaagaaaga ctccaccgcc gagctgtacc acttcatcgg taaagatatt
     2761 gtttacttcc acagcctgtt ctggccagca atgctggaag gcagcaactt ccgcaagccg
     2821 accaacctgt ttgttcatgg ctatgtgacg gtgaacggcg cgaagatgtc taagtctcgc
     2881 ggtaccttta ttaaagccag cacctggctg aatcattttg acgctgacag cctgcgttac
     2941 tactacactg cgaaactctc ttcgcgcatt gatgatatcg atctcaacct ggaagatttc
     3001 gttcagcgtg tgaatgccga tatcgttaac aaagtggtta acctggcctc ccgcaacgca
     3061 ggctttatca acaagcgttt tgacggcgtg ctggcaaacg aactggctga cccgcagttg
     3121 tacaaaacct tcactgatgc cgctgaagtg attggtgaag cgtgggaaag ccgtgaattt
     3181 ggtaaagctg tgcgcgaaat catggcgctg gctgatctgg ctaaccgcta tgtcgatgaa
     3241 caggctccgt gggtagtcgc aaaacaggaa ggtcgcgatg ctgacctgca ggcgatttgc
     3301 tcaatgggca tcaacctgtt ccgcgtgctg atgacttacc tgaagccggt actgccgaaa
     3361 ctgaccgagc gtgcagaagc cttcctcaat acggaactga cctgggatgg tatccagcaa
     3421 ccgctgctgg gccacaaagt gaatccgttc aaggcgctgt ataaccgcat cgatatgaaa
     3481 caggttgaag ctctggtgga agcctctaaa gaagaagtga aagcagctgc cgcgccagtt
     3541 actggcccac tggcagatga tccgattcag gaaaccatca cctttgacga cttcgccaaa
     3601 gttgacctgc gcgtagcgct gatcgaaaac gcagagtttg ttgaaggttc tgacaaactg
     3661 ctgcgcctga cactggatct cggcggtgaa aaacgcaatg tcttctccgg tattcgttct
     3721 gcctacccgg atccgcaggc actgattggt cgtcacacca ttatggtggc taacctggca
     3781 ccacgtaaaa tgcgctttgg aatctccgaa ggcatggtga tggctgccgg tccaggcggg
     3841 aaagatatct tcctgctaag cccggatgct ggtgctaaac cgggtcatca ggtgaaataa
     3901 tcccccttca aggcgctgca tcgacagcgc cttttcttta taaattccta aagttgtttt
     3961 cttgcgattt tgtctctctc taacccgcat aaatactggt agcatctgca ttcaactgga
     4021 taaaattaca gggatgcaga atgagacact ttatctatca ggacgaaaaa tcacataaat
     4081 tctgggcggt ggagcaacag ggaaacgagt tgcatatcag ttggggaaaa gttggcacca
     4141 aagggcaaag tcagataaaa agtttttcag atgctgcggc agcggaaaaa gcggaactta
     4201 agctgattgc ggagaaggtg aagaaggggt atgtggagca agcgaaggat aattctttgc
     4261 aaccttccca aacggtaacg ggctctctca aggtagcgga cttatccacc attattcagg
     4321 aacaaccctc tttcgtagca gaaacccgtg cgcctgacaa aaatacagat gctgttttac
     4381 cgtggctggc gaaagatatt gctgtcgttt ttccgcccga agttgtacac accacgttaa
     4441 gtcatcgccg ctttcccgga gttcctgttc agcaagcaga caaattgacc caattacgtc
     4501 gcttagcctg tagtgtgtcg caacgggata ataaaacagc cacatttgac ttcagcgcct
     4561 gttctttaga atggcaaaac accgtcgccc aggcgatcag tcagatcgac ggcctgaaaa
     4621 caacacagtt accatcacca gtaatggctg tactcacggc acttgaaatg aaatgcacaa
     4681 gatataaagt gcgtgaggat gttatggatc agatcgtcca ggaaggcggt ctggaatatg
     4741 ctactgatgt aataatacac cttcaacaga ttgatattga atgggattat gcgaataatg
     4801 tcattattat tctgccgtct ggcattgcac ctagctactt ggagcaatat tccagatttg
     4861 aattacgcct acgtaaacat ttatcactga cggaagagtc tctctggcaa aaatgtgcac
     4921 aaaaacttat tgccgcaatt ccacatattc cagaatggcg gcaaccatta attgctttgt
     4981 tattacccga aaaaccagaa attgcacatg aaattgccca gcgtctactg gggcaaaaaa
     5041 aattaccctc gcttgagtgg ttaaaaatag tggcgactga tgagcacatt cttgcctcat
     5101 tagaaaaata tcacgaacca tatgccattt ttgatgatta ctattgtggt gcgatatggt
     5161 cagccaccgt attacaggag caaggtgttg cagccctgcc ccgatttgct ccctatgccg
     5221 caagtgacta ctgcgccgat gtgttgcgtc atatcaatca tccgttcgca ttgacactgc
     5281 ttatacgtgt agccgggcaa actaaacgct gtcacgatcg gatgacgaaa gccattgctg
     5341 cgttcccaca tgcagcaatg gcggcactga cggaacttct tgggcaaaaa gaagagaaca
     5401 gttggcgcat tatgctaatg acaatgctta tctcacaacc agcactggca gaacaggtca
     5461 ttccctggct ctcgacaccc gcagttgccg tactgaaatc atgccagcaa caactgacac
     5521 agccctcaaa ccatgccagc gccgatctac tgccagccgt agtagtctcc cctccctggc
     5581 tttcgaaaaa gaaaaaatcg ccgattccgg tgctggattt agcgccatta ggcattgagc
     5641 caatctgtta tctgacagaa gaaatcagta atcaactttt ggcgaaatat atctggtatt
     5701 caaaacacat cacggttagc catgaagaaa gtactaccaa cctgttggca aggatgggtt
     5761 ttcaacgacg gatcgctggt acatatatta aagctcccga agcggtagtt gaggcatggc
     5821 taaatgaaga ttattcaacc ttactaagtg aatttaaggt gtttcattca cctaccgggc
     5881 attattggca gttggggatt ttgacaacat tgccgctgga gaaagcagta aaagcatgga
     5941 atgcccttac cctatctcca cataccgata ccgaatactc catgttacat tttggactca
     6001 aagggttacc tgggttagta aactcacttg cacgctatcc acaagaagcc ttgcccatca
     6061 cgaattactt cgcagcgagt gagctggcac ctgccgtcgc ccgcgccttc aacaaactca
     6121 aaaccctacg acaagacgcc cgtagctggc tgctaaaata cccggaacat gccataaccg
     6181 gcctgctacc tgcggcgctc ggcaaagccg gtgaagccca ggataacgcc cgcgctgcct
     6241 tgcgtatgct taccgaaaac ggtcatcagc cattactgca agaaatcgcc cggcgttata
     6301 accagccgga agtaacagac gcggtgaacg ctctgcttgc gctcgatccc ttagataatc
     6361 acccgacaaa aatccccact cttccggcct tttatcagcc atcgctctgg acgcgcccgg
     6421 tattaaaagc aaacgcccaa tcactgccag ataacgccct cctccacctc ggtgaaatgc
     6481 tccgcttccc tcaggaagag gctctgtatc cgggattatt gcaggtgaaa gatgcctgta
     6541 ccgccgactc actggctgaa tttgcctggg atctgtttac cgcctggcag accgctggcg
     6601 cgccgtcgaa agaaagctgg gcgttcactg cgttaggcgt tctcggtaac gatgacaccg
     6661 cccgcaaact gacgccatta atccgcgcct ggcctggcga atctcagcat aaacgtgcca
     6721 ccgttgggtt ggatattctc gctgctatcg gtagtgatat cgccctgatg cagcttaacg
     6781 gcatcgccca gaaactgaaa ttcaaagcat tacaggagcg ggcaaaagaa aaaattgccg
     6841 acattgccga aagtcgcgaa ctcacggtgg cggagcttga agatcggtta gcaccggatc
     6901 tcggtctgga tgataacggt tcgctgctgc tggatttcgg cccacgtcag ttcaccgtca
     6961 gctttgatga aaccttaaaa ccgtttgtgc gcgatgcttc cggcagccgc ctgaaagacc
     7021 tgcccaaacc aaacaaaagc gatgatgaat cgcgggctga tgaggcggtt aaccgctaca
     7081 aactgctgaa aaaagatgcg cgtaccgtcg ccgcccagca ggtggcaagg cttgaatccg
     7141 ccatgtgcct gcgccgccgc tggtcaccag aaaacttcca gctcttcctg gttgagcacc
     7201 cgatggttcg ccacttaacc cgacgtctga tttggggcgt ttatagcgcc gacaaccagc
     7261 tacaggcgtg ctttagggta gcggaagata acagctacag caccgctgac gatgatcttt
     7321 tcaccctgcc ggaaggcgat atctctcttg gtattcctca cgttctggaa atatcaccga
     7381 cggatgctgc cgcctttggt cagctttttg ccgactacga actgctacca ccgttccgtc
     7441 agcttgaccg taacagttac gccctgacag aagtcgagcg caatgccagc gaattgatcc
     7501 gctgggcagg cagaaaatgc ccgagtggtc gggtaatggg gctggcgaat aaaggctgga
     7561 taaagggcac cccgcaggat gcaggctgga tcggctggat gatcaaacct ctcggtcgct
     7621 ggtcgttaat catggaaatc gatgaaggct ttgcggtagg catgtcgcca gccgaactca
     7681 gcgctgagca gctcttaagc aagctgtggc tatgggaagg caaagcagaa agatatggct
     7741 gggggagtaa ttcaacacag gaagcgcagt tctccgtaat cgatgccatc accgccagcg
     7801 agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac aaggaattac
     7861 cgtggctggt ggataacgcc caactggaac tgaaatataa aaaaggcaaa acgccgctca
     7921 gtcatcgtcg ctggccgggc gaaccagtgt ccgttatcac tggaagtctc atccagacat
     7981 tgggtgatga attgctacaa aaagctgaga agaaaaaaaa cattgtctgg cgttatgaga
     8041 atttttcact ggagtggcag tccgccatca cgcaggccat caacttgatc ggcgaacaca
     8101 aaccctcagt cccggcccag acaatggcgg cgctagcctg tatcgcgcaa aatgacagcc
     8161 aacagttgct cgacgaaatc gtccaacaag aggggctgga atatgcgact gaggtggtga
     8221 ttgcacgcca gtttattgcg cggtgttatg agagtgatcc tctggtagtg acattgcagt
     8281 atcaggacga ggattatggc tatggttatc gctcagaaac ctataacgaa ttcgatctcc
     8341 gactgcgtaa gcatctctct ctggcagagg aaagctgctg gcagcgttgc gccgacaaac
     8401 tcattgccgc actaccagga ataaccaaag ttcgccgccc ttttattgcg ctgatcctcc
     8461 cggaaaaacc agaaatagcc aatgagttgg taggccttga atgcccgcga actcattttc
     8521 attctaagga gtggttaaaa gttgttgcta atgaccccac agcggtgaga aaactcgaac
     8581 actactggag ccaggatata tttagcgatc gagaagccag ctacatgtcg catgaaaacc
     8641 acttcggcta cgcggcctgc gccgcccttt tgcgcgaaca aggactggca gccattccgc
     8701 gcctcgcgat gtatgcccat aaagaagatt gcggcagtct gctggtacaa attaaccatc
     8761 cgcaagtcat ccgcaccttg ctactggtgg ctgataaaaa caaacccagc ctgcaacgtg
     8821 tagctaaata ccataaaaac ttcccccatg cgacgctcgc cgcactggca gaactgctgg
     8881 cgttaacaga accaccagcc cgccctggtt atccaatcat cgaagacaaa aagctgcctg
     8941 cacagcaaaa agcacgcgat gaatactggc gtacgctgtt acagacgctg atggcatcgc
     9001 agccacaact ggcagaagag gtgatgcagt ggttaagtac tcaagccagg gcagtgctga
     9061 atagttattt atcggcaccg cccaaaccgg ttattgatag taccgataac agcaatctgc
     9121 ctgaaatcct cgtttcgcca ccgtggcgta gtaagaaaaa aatgacagct ccacgtcttg
     9181 atttggcacc gctcgaatta actccgcaag tttactggca accaggcgaa caagagaggc
     9241 ttgccgccac tgagtctgcc cgttatttca gcacggaatc tcttgcgcaa cgcatggaac
     9301 aaaaaagtgg acgagttgta ttacaggaac tgggttttgg ggatgatgta tggctgtttc
     9361 tgaattatat actccccgga aaactggatg ctgcacgcaa ttcactcatt gttcagtggc
     9421 attactacca ggggcgggtt gaagagatcc tgaatggctg gaactccccg gaagcacaat
     9481 tagcagaaca ggcgctccgc agcggtcaca tagaagcgtt aattaacata tgggaaaatg
     9541 acaactactc acgttatcgt ccggaaaaga gtgtctggaa cctgtattta ttggcacagt
     9601 tgccgcgtga gatggctttg accttctggc tgcgtatcaa tgagaaaaag catctgttcg
     9661 cgggtgagga ctattttctc agtatcctcg gattggatgc gctaccaggt ctgctgttgg
     9721 ctttttcaca tcgtccaaaa gaaacatttc cgttaatttt aaatttcggc gcaacagaac
     9781 tggccctgcc cgttgcccgc gtctggcacc gttttgcggg ccagcgtaat ctggctcgcc
     9841 agtggatttt acaatggccg gaacatacgg ctactgcact tattccactc gtctttgtta
     9901 aaccctgcga caacagcgaa gcggcattat ttgcccttcg tttactgtat gaacaaggac
     9961 atagtgaatt actgcaaacg gttgcaaacc gctgggatcg cgctgatatg tggccagccc
    10021 tggaaaaaat acttacccag aacccgatgg aaatttaccc ggcacgcatt ccaaaagccc
    10081 ctgatttctg gcatccgcaa atgtggtcca ggccgcgcct tatcactaat aatcaaactg
    10141 ttaccaatga cgctctggaa attatcggcg aaatgctgcg ctttacccag gggggacgtt
    10201 tttatagcgg gctggaacaa ctgaaaacgt tctgccagcc acaaacgctg gcagcttttg
    10261 cttgggatct cttcactgcg tggcaacaag ctggtgcccc cgcaaaagac aactgggcat
    10321 ttctggcgtt aagtctcttt ggtgacgaaa gcacggcacg ggatctgacg acacagatcc
    10381 tcgcctggcc acaagaaggc aaatctgccc gtgctgtcag cggcctgaac atccttaccc
    10441 tgatgaataa tgatatggcg ctgatacagc tgcatcatat atcgcaacgg gctaaatccc
    10501 gccccttacg tgataacgcg gcggaatttc ttcaggtggt cgcagaaaat cgcgggctaa
    10561 gccaggaaga gctagcggac agattagtcc caaccctggg ccttgatgat ccgcaggcgt
    10621 tgagttttga ttttggtccc cggcagttta ccgttcgctt cgatgaaaat ctcaacccgg
    10681 ttatctttga tcagcaaaac gttcgccaga aaagcgttcc ccgtttgcgc gccgatgacg
    10741 atcaactgaa agcgcccgag gcactggccc gactaaaagg gctaaaaaaa gatgctactc
    10801 aggtgagcaa aaacctgctc ccgcgtcttg aaacggccct acgtaccacc cgacgctggt
    10861 cgctggcaga ttttcattct ctgtttgtta atcatccctt tacccgtctg gttacccagc
    10921 gattaatatg gggggtttat ccggcaaatg aaccgcgttg tttactcaaa gcctttcgtg
    10981 tggccgcaga gggggagttc tgcaatgcgc aagatgagcc aattgacctg cctgcggacg
    11041 ctctgattgg cattgcccac ccgttagaaa tgacagcaga aatgcgcagt gaatttgcac
    11101 agctttttgc cgattacgaa attatgccgc cttttcgcca gttgtcgcgc cgcacggtgc
    11161 tgctcacacc tgacgagtca accagtaaca gcctgactcg ctgggaaggt aaatccgcta
    11221 ccgttgggca acttatggga atgcgataca aaggctggga gtcaggctat gaggacgcat
    11281 ttgtctataa cctgggtgag taccggctgg tccttaagtt ttcacccggt tttaaccact
    11341 acaatgttga tagcaaagcg ctaatgagct tccgttctct tcgagtgtac cgtgacaata
    11401 aatccgtcac ttttgccgaa cttgatgtgt ttgatttgag tgaggcgtta agcgcacctg
    11461 acgtcatttt ccattaaaac acagcgggca gtgatgcaac tgcccgttat caacacgact
    11521 taccagcggg gaaagcgatg atcgtgcaaa aagagctggt tgctatttac gattatgagg
    11581 tccctgtacc tgaagatccg ttttccttca gacttgagat ccataaatgc tctgaattat
    11641 ttacaggttc cgtctatcga ctggagcgat tccggctacg tccaacattt catcaacgtg
    11701 atcgagaaga tgctgacccg ctaataaatg atgcgttgat ttatataaga gatgagtgta
    11761 ttgatgagcg gaaattacga ggtgaatcac ctgaaactgt aatagcaatt tttaatcgtg
    11821 aactacagaa tatattcaac caagaaatag aataatatac tctaaataat tcaaattggt
    11881 ccgatccggc gcaacgtccc aatggcctgg attataaatc tcattatctt aattgcaacg
    11941 gggtccagcc gtggttataa ttcgttcgtt ggaacacgat gttgcacagg ctgtggtgtt
    12001 aggcctgaaa atagtaaata aaagaacctg cctcaccagc aggttttttt atttactgtg
    12061 atctgctttc cagatatttt tcgctcaaac aactaatgcg ccaaacattt attgcgcgta
    12121 aaatatcgtt tatttcatta atacatttca gggatgaata tatgtcaccg cagaataatc
    12181 atctgcagcg tccgcctgct gctgtgttat acgccgatga actggcaaaa ttaaaacaaa
    12241 atgataacgc accttgcccg cccggttggc agttaagttt gcctgcggcc cgtgctttta
    12301 tccttggcga cgaagcgcaa aatatcagcc gtaaagtggt gattagcccc tccgctgtcg
    12361 aacgtatgtt agtcactctt gctaccggcc gcggtttgat gttggtgggg gaaccgggta
    12421 ccgcaaaatc tcttctttct gaattactgg caaccgctat tagcggcgat gccgggttaa
    12481 ccatccaggg cggggcatct accaccgaag atcaaatcaa gtatggctgg aactacgccc
    12541 tgcttatcaa tcatggtccg tcaacggaag cgctggtccc tgccccgctt tatcagggaa
    12601 tgcgcgacgg caaaatcgtc cgtttcgaag agataacgcg tacaccgctg gaagtgcagg
    12661 actgtctgtt aggtatgctt tctgacaggg tgatgacggt gccggaactc actggtgaag
    12721 ccagtcagct ctatgcgcga gaaggcttta atattattgc cactgccaat acccgcgatc
    12781 gcggcgtcaa tgaaatgagt gctgcgctaa agcgtcgctt cgattttgaa acggtttttc
    12841 cgattatgga tttcgcccag gagttggaac tggtcgccag tgcttcggcg cgtttgctgg
    12901 cccatagcgg tattccgcat aaagtgcctg acgcggtact ggaattactg gtccgcacct
    12961 tccgcgatct gcgtgccaac ggcgaaaaga aaacgtcaat ggatacactg acagcgatta
    13021 tgtccaccgc tgaagccgtg aacgtggccc atgctgtggg cgtccgcgcc tggtttttag
    13081 cgaatcgcgc gggggagcct gccgacctgg tggaatgtat tgccggaacc atcgtcaaag
    13141 ataacgaaga agatcgcgcg cgtctgcgcc gttacttcga acagcgtgtc gccacacata
    13201 aagaagctca ctggcaggct tattatcaag cccgccaccg cctgccgtga ggaaagatgc
    13261 atgagcgagc cgttaattgt cggcatccgg catcatagtc cggcctgcgc ccggctggtg
    13321 aaatcgttaa tcgaaagcca gcggccacga tacgtgttga ttgaaggccc ggctgatttt
    13381 aatgaccggg tagacgaact gtttttagcc caccagcttc cggtagctat ttacagttat
    13441 tgccagtatc aggacggtgc agcccccggg cgtggtgcct ggacgccatt tgctgaattt
    13501 tcgccggagt ggcaggcgct acaagccgca cgtcgcattc aggcacaaac ttacttcatc
    13561 gatttgcctt gctgggcgca gagtgaagaa gaggacgatt cgcctgatac gcaagatgaa
    13621 agccaggcct tactgctgcg tgccacccgc atggataaca gcgataccct gtgggatcac
    13681 ttgttcgaag atgaaagcca gcaaactgca ttaccctctg cgctggcgca ctattttgcc
    13741 caactgcggg gcgacgcctc cggcgatgcg ctcaatcgtc agcgcgaagc ctttatggcc
    13801 cgctggattg gatgggcgat gcagcaaaat aatggcgacg tgttagttgt ctgcggtggc
    13861 tggcacgctc cggcactggc aaagatgtgg cgcgaatgcc ctcagaaaat taacaagcca
    13921 gaattgccct cgctggcaga tgccgttaca ggttgttatc tcacacccta cagtgaaaag
    13981 cgccttgatg tgctggcagg atacctttca ggaatgcctg ccccggtatg gcaaaactgg
    14041 tgctggcagt ggggcttgca gaaggccggt gaacaactgc taaaaactat ccttacccgt
    14101 ttgcgccagc acaaattgcc cgcttctacc gcggatatgg ctgccgctca tctgcatgcg
    14161 atggcgctgg cacagttgcg cggtcataca ctaccgttac gcactgactg gctggatgcc
    14221 atagcaggct cgctgattaa agaagccctg aacgcgccgt tgccgtggag ctatcgcggc
    14281 gttattcatc ccgataccga tccgattctg ctaacgttga tagacacatt agcgggtgac
    14341 ggattcggta aacttgcccc ttctacgcca caaccgcctc tgccaaaaga tgtcacctgc
    14401 gaactggaac gtaccgcaat ctcccttccg gcggagctta ccttaaatcg ctttaccccc
    14461 gatgggctgg cgcaaagtca ggtgttacat cggctggcaa tactggagat ccctgggatt
    14521 gtacgccagc agggaagtac actgacactt gcaggcaacg gtgaagaacg ctggaaatta
    14581 acccgcccgc ttagccagca tgcggcattg attgaggccg cctgttttgg tgccacactc
    14641 caggaagccg cacgcaataa attagaagcc gatatgctgg acgcgggcgg aatcggcagt
    14701 atcaccacat gtcttagcca ggcggcgtta gcgggtctgg cgtccttcag tcaacaatta
    14761 ctggagcaac tcacactatt aatcgcccag gaaaatcaat ttgccgaaat gggccaggcg
    14821 ctggaagtgc tttatgcctt atggcggctg gatgaaatta gcggtatgca aggcgcgcag
    14881 atattacaaa cgacattatg cgcgactatc gatcgcacgc tgtggctgtg tgaatctaac
    14941 ggcagaccgg atgaaaagga gtttcacgct cacctgcata gctggcaagc gctttgccat
    15001 attctgcgcg atctacatag cggcgttaat ttacccggcg tttctctttc tgcggcggta
    15061 gccttactgg agcgacgcag tcaggcaatt catgccccgg cgctggatcg cggcgcggct
    15121 cttggcgcac taatgcgtct ggaacatccc aacgccagtg ccgaagcggc gctgacgatg
    15181 ctggcgcagt tatccccggc acaatctggt gaggcgctgc acggtttgct ggcgctggcc
    15241 cgccatcaac tggcctgtca gccggcattt atcgccggtt tcagcagtca tttaaatcaa
    15301 ctgagtgaag ccgattttat taacgccctg cccgatttac gcgcggcgat ggcctggcta
    15361 ccaccacgag aacgcgggac gctggcgcat caggtgcttg agcattatca actggcgcaa
    15421 cttcccgttt cggcgctgca aatgccgttg cattgtccac cacaggccat tgcacatcat
    15481 caacaactcg aacagcaggc actggcatcg ctgcaaaact ggggagtttt ccatgtctga
    15541 actgaacgat cttctgacca cccgtgagct acaacgctgg cgattaattc ttggcgaagc
    15601 ggcagaaacg acgctttgtg ggctggatga caacgcccgg cagatagacc acgcgctgga
    15661 gtggctgtat gggcgcgatc ctgaacggct ccagcgtggt gaacgttccg gtggattagg
    15721 tggctcaaat ctcaccaccc ctgagtggat caacagtatt cacacgctgt ttccgcagca
    15781 ggtgattgag cggctggaaa gcgatgccgt gctgcgctac ggcattgaag atgtggtgac
    15841 gaatctcgac gtgctggaac gtatgcagcc ttctgaaagc ctgctacgcg ctgttttgca
    15901 caccaaacat ctgatgaacc ccgaagtact ggctgccgcc cgccggatag tgtgccaggt
    15961 tgttgaagaa attatggctc gactggcaaa ggaagttcgt caggcttttt ctggtgtccg
    16021 cgatcgccgt cgccgttcat ttattccact ggcgcgaaac tttgatttca aaagtactct
    16081 gcgcgccaac ctgcaacact ggcacccgca acacggcaag ttgtatatcg aatccccccg
    16141 ctttaacagc cgcattaaac gccaaagcga acaatggcaa ctggtcttac tggttgatca
    16201 aagcggatcg atggtcgatt cggtgatcca atctgcggtg atggcggcct gtttgtggca
    16261 gttacccggc attcgtaccc atctggtggc gtttgacaca agcgtcgttg atctcacggc
    16321 agacgttgcc gatccggtag agttattaat gaaagtacag ttgggcggcg ggaccaatat
    16381 cgccagtgcc gtggagtatg gtcggcaact tattgaacaa ccagcgaaaa gcgtcattat
    16441 cctcgtgagc gatttttacg aagggggttc atcatcatta ctgacgcatc aggtgaaaaa
    16501 gtgtgtccag agcggcatca aagtgctggg actggcagcg ctcgatagca ccgcaacacc
    16561 ttgctatgac cgcgatacgg cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat
    16621 gacgccgggc gagctggcat catggcttgc ggagaatctt cagtcatgaa ttcactacgt
    16681 ccggaattat tagaactgac accgcaggcc ctgacggcgt taagcaatgc cggttttgtt
    16741 aagcgcagtc ttaaggaact ggaaaatggc aacgtcccgg agatcagcca tgagaacgac
    16801 gctttaatcg ccaccttcag tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg
    16861 aaagaggctc agtgcagttg cggggccaac ggtatgtgcc gtcatcgcgt gatgctggtg
    16921 ttaagttatc aacgactttg tgccaccact cagtctacgg aaaaagaaga agagtgggat
    16981 ccggcaatct ggctggaaga actggctacc cttcccgatg ctacccgcaa acgcgcacag
    17041 gcgctggtcg ctaaaggcat caccattgag ttgttctgtg cgccgggtga aattccctct
    17101 gcccgcttac cgatgagcga tgtgcgtttt tattcccgca gcagtattcg tttcgcccgt
    17161 tgtgattgta ttgaaggcac actttgcgaa catgtcgtac tggcggtaca ggccttcgtc
    17221 gaggccaaag cgcagcaagc agaatttaac catttaatct ggcagatgcg cagcgaacac
    17281 gtcacatcat ctgacgatcc gtttgccagc gaagaaggca acgcgtgtcg tcaatatgtt
    17341 cagcaattaa gccagacatt atggcttggc ggcatcagcc agccgctcat ccattacgag
    17401 gcagcattca accgcgcatt gcaggcggca gagacctgca actggcgctg ggtgagtgaa
    17461 tcgctacggc aactgcgcgc cagcgttgat gccttccacg cccgcgccag ccactataat
    17521 gccggagaat gcttacatca gcttgcggca ttaaacagtc gattaaattg cgcacaagag
    17581 atggcccggc gcgacagtat tggtgaagtt cctcctgtgc cgtggcgcac ggtcgttggc
    17641 tctggcattg ccggagaagc aaagcttgat catctgcggc tggtgtcttt aggtatgcgt
    17701 tgctggcagg atattgagca ttatggttta cgcatctggt ttaccgatcc cgacaccggc
    17761 agtattttgc acctttcgcg cagttggccg cgaagtgaac aggaaaactc accggcagct
    17821 acgcgtcggc tgtttagttt tcaggctggc gcactggcgg gcgggcaaat tgtttcacaa
    17881 gcagcaaaac gcagtgccga tggcgagctg ctgttagcta cccgcaaccg cttaagcagc
    17941 gttgtgccgc tgtcgcctga tgcctggcaa atgttgagcg cgccgttacg ccagccgggc
    18001 attgtggctt tgcgggaata tttacgccag cgtccccccg cctgcatacg gcctcttaat
    18061 caggtcgata acttatttat tctgccggtc gctgagtgta tttcgctcgg ttgggatagc
    18121 agccgccaga cgctggatgc gcaggtaatc agcggcgaag gtgaagataa tctgctgacg
    18181 ttatcactgc cagcctcagc cagcgcgcct tatgccgttg aacgcatggc ggcgcttttg
    18241 caacaaacag acgaccccgt gtgtctggtt tctggctttg tcagttttgt tgatgggcaa
    18301 ttgacactgg aaccacaggt aatgatgaca aagactcgtg cctgggcgct ggacgctgaa
    18361 actgcgcctg tggcaccact accttctgcc agcgttttgc ctgtgccgtc tactgctcat
    18421 cagttgctga tgcgctgcca ggcgttactt attcaactgc tccataacgg ctggcgctat
    18481 caggaacaga gcgctattag tcaggcagag ttgctggcga atgacctcac cgcggtgggt
    18541 ttttatcggc tggcacatgt gttggggcaa tttcgtaata cagaaagcga ggcacgggta
    18601 gaagcaataa ataacggtgt tttgctttgc gaacaattat tccccatgct tcagcaacaa
    18661 ggatgaaata gtgcttttta ctaagagctc tactccagtt ccggactgct cacgccacgg
    18721 tattaggcat atcctatata gcccctggtg agagtcacca gtttcttgat taaataaaat
    18781 ggagttttac atgaaggctt tcaataagct gttttccctc gttgttgcat ctgttctggt
    18841 tttctctctt gctggctgcg gtgacaaaga agaatcgaag aaattcagcg ccaatctgaa
    18901 cggcactgaa attgccatta cctatgtcta caaaggtgac aaggtgctta agcaatcttc
    18961 tgaaaccaaa attcaatttg cctccattgg tgcaaccacc aaagaagatg ctgccagggc
    19021 acttgagccg ttaagcgcca aatacaaaaa catcgcgggt gttgaagaaa aattaaccta
    19081 taccgatacc tacgcgcagg aaaacgtgac tatcgatatg gaaaaagtgg atttttaaag
    19141 ccctgcaggg tatttcagga atcaacgttt ctgctgaaga tgccaaaaaa ggtatcacta
    19201 tggcgcaaat ggaactggtg atgaaagccg ctggtttcaa agaagtgaaa taatctgtcg
    19261 gcggccatgt ttcgcatggc cgccataccc gtcttagctt ttcttcacat gctggcgcgc
    19321 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
    19381 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
    19441 gaggatcgcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt tttttagcac
    19501 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
    19561 tttttcataa atcaggccat tgaggaatga cgacaaaaca atgtccggac aacgctgaaa
    19621 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
    19681 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
    19741 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
    19801 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
    19861 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
    19921 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
    19981 tttccagggt acgtaatgtc aattcggtaa atccctcttt cccttcgtgg ctggtaacgt
    20041 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
    20101 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
    20161 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
    20221 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaatt gcgtattcgt
    20281 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc cccaccattt
    20341 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
    20401 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
    20461 gctcctgcaa aaatacacgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
    20521 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
    20581 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
    20641 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
    20701 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
    20761 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
    20821 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
    20881 gaagcgcgcc ttttcaattt gcagataagc attcacatgt tcaatttcgt cggcgagagt
    20941 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
    21001 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
    21061 caaaaaatgg ggattcacct gggcgtgaag cagtttgatc tctgactggg tgagcatcgc
    21121 tttttgccgc tcatattgcc cggcaaggat ctgcgccgaa agcaattgcg caatcccctc
    21181 acccaacgtg cggttgattg aactgaataa acggtttttg gcttcataca atttgatggt
    21241 gcccatcacc cgctgatttt caccacgcaa cggaattacc agcgtcgacc ccagtttgca
    21301 ttgcggatgc aaagagcaac ggtaaggtac ttcgttgcca tcagcgtaga ccacttcacc
    21361 ggtttcaatc gcttttaaag tataagttga agaaatcggt ttgccgggta aatggtggtc
    21421 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
    21481 atccagttcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
    21541 gcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagccgaagt
    21601 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
    21661 ggtattggtg accatcattg gcgcagcaat attactcacc agacgcaccg catcttcata
    21721 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
    21781 accggcggta atggggttaa agactttatc agtgcgcccg cggcggatca ggatgctgtg
    21841 taccaggcca ccgagtaatc cttcaacgat ggtcgagatc atgcaactta acgcggtcat
    21901 gccccccatc gaatatcgat gtaagccgcc ggtcagccca accagcccac cgacgaccgg
    21961 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
    22021 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
    22081 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
    22141 cattaaccat gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
    22201 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
    22261 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
    22321 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
    22381 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
    22441 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa tcctgtcacg ttacgcgcgt
    22501 gacgcaacat ttttaaaaat caacgaagat cgtccaggag cgccgatttt caagggttgg
    22561 acagataatt cgttttcgat gtcgacgaac ttcgacggat ttcgcctata aaaagtgtcc
    22621 catacatgcc ccattgatgc cccatagaca atatcagcaa ttgacactgg ttatctatac
    22681 aactaaaaat aacacctgta cataaccaca gttacaggag ggtgttatgc gtgttgagag
    22741 ctgtatcgct aaagaaaaaa tcagcaaaat gccaaaaggt tctgttcctg ccctgcaaca
    22801 ggaaatgctg cgccgtatca ggaaacgata tgacaatgca gaaatcatca tcaaatctgc
    22861 cagtaatgat ggactcacta ttttaagagc cacagataaa gagtccgcac acaaatttgt
    22921 acaggaaaca cttcaggaag ccagggaaac agcggacgac tggtttattc actgactaaa
    22981 atcccgtcca catgccggga atttcctatg tccgaagaaa atggtgtggt taactaagca
    23041 aaagtgtccc tttatcggta tagcaactca ttttgttaac atacggttgc aaaattgatg
    23101 acaagtgttt attttatcca caagaaatgt atgcaaacca gaattgggaa atgcatttat
    23161 aaaataagga atagcagata tgtttgcagc aaagcccgaa ttaagtcaaa gatgtatttc
    23221 tggactcctc acaactgggc caaatgatga aaacgtaaat aataaaccgt caagtgatat
    23281 gccgaacaga atcaaaccag ttgtaagcga ttcacaaatc ggtatatata caactgacag
    23341 taatactctg aaggatggat tgtgtagcca agcccacata ggaactcaac cttatcggcc
    23401 aggtgctgac tcgccgataa ctgtgagatc attcctacaa atcacacttt ccaacaataa
    23461 ttacaaatca ttagagcatc tgcaaacacg agcagagaat tatctgaggt atcgcaaagc
    23521 agaagagaat attctgcgtt cgaccgttga ggggctaacc aatccagaat ctcctgtatt
    23581 caaacaaaca gcatggatgg ggcatctgga gcgtggcctt tggaaaacgg aaacacgctg
    23641 ggatggaaac gaccgcgagc agctaggcaa agaggctctt ggctcagagg agccaaaacc
    23701 tggttcacca ttttacggta gtcgtggact aaaattatcc gacagtgcgc attctgcctt
    23761 ctcaatgatg ctgtgcggtt ctgaaggtcc attcacaaaa gaacaagcac tctctggttt
    23821 cgagcttgct caaactggcc aagtgctggc tggaaggcta aagattcagg agcgagttaa
    23881 attccgcgca gacaaccgca ttgatgctca gcgtaacggt acacactcaa cccgcacacc
    23941 aacaggcatg gatctttctc aagatatcgg cacaattatg cgtgataaag ctgggctacc
    24001 ggtcatgagc ggaacatccg gttcctcctc tgatgccact cttgcaacac gttacgctgc
    24061 cgagcatttt ggtaagacat gggccgctcc aggcttaagc caagcagaag gatgcaaagc
    24121 tatctcggat ctgtcacatc actattttcg tgcagaaggc tccagtccac cacaatcaat
    24181 ggcaatggca acgggaataa ataaggttcg ttatgatgca ggaatggaag agaaatatgt
    24241 aaatacactg gatatattta cacatagcta cccagaaatt tatgctgggg ttgcattgac
    24301 gattgctggg gctggcggta atgatgagca ggcaatgtat aatgttacgc aagaagccgc
    24361 tcgtattctg cacgaagctg aaactaaaga ttgaacatgt tctagtgaag aggcctaccc
    24421 cagaagaata tattccacat tcaaggcatt attgatgaat tttataatca atgcgaaaaa
    24481 actggcattg attgtataca gattctcctg aaggagaatc tgttctgtcc cttctgccaa
    24541 gcagtgttac gcaacgatca atctttcgcg ttcctattaa tcaatgagtt agagttgatt
    24601 agtgacctga gacagagcat tagcgcaagg tgattttttg tcctcttgcg ctaatttttt
    24661 gtcatcaaac atacagccaa tcagaagggc ataaagctgc caagcattat atgtcttagt
    24721 tttatgctca ttttcattaa aaatatatat aataagacta atatcagtat aaaacattta
    24781 taacacttag agtaaaaatg caatcagctt atacaatcaa caataattta aaccataaaa
    24841 aacaatagta gaaacattta taatttattt taagtattca aacgataaac tggtaaattt
    24901 aacaacttaa ttttgagatc taagtcacat caatcaagca ttcaaacagc tatatattaa
    24961 agctgtccac atcggatatg tgacactgat aatatcaatg gattgatatt attaatggat
    25021 ataaattacg tcataaggat ttatcatgaa cattcaaccg accatacaat ccggaattac
    25081 cacacaacac aatcaacatc atcacacaga acaaacgccc ccaacacaaa taccgcaatc
    25141 cgaattacct ctaggatgcc aagctggatt tgttgttaat attccagatg atatacagca
    25201 acatgcaccg gaatgcggtg aaacaacagc tctactgagc ttgataaaag ataaaggtct
    25261 gctctcaggg ttagacgcat atattgctcc tcaccttgaa gaaggctcag caggaaaaaa
    25321 agcattggat atgtttggtt tattcaatgt tactcagatg gcattagaaa tacccagcac
    25381 cgttcctggt atctctggta aatatggtgt tcagatgaac attgtaaaac cagatattca
    25441 ccccacaacc gggaattatt ttttacagtt attcccccta catgatgagg ttggaataaa
    25501 cttcggtgcg ctcccaggac cactccagaa gtttctctct caaaatccca atccatggtt
    25561 tggagtaaca gctcaagttg ttcgtaacca cggcatagaa ctacctatag tcaaaacaga
    25621 taatggatgg aaactagttg gagaaacacc acttacacct gaagggccta aagcaaatta
    25681 tacggaagaa tgggtcatca gacctggtga gcttgactta aagtatggca gcactccgtt
    25741 tcagagcact ttaggtttag aatttggagc tcattttaaa tgggatttag ataatcctaa
    25801 taccaaatat gccgttctta ccaatgcact tgcgaatgca gctggtgctg cagggggata
    25861 tactctatct caatatacag gaacaaacgc aatgctgagc ccccatattg gctcgatggc
    25921 cggacaggct ttggggcatg ctgtacaatg ctacactcca ggattaaagc cagacactat
    25981 tttatggtgg gcaggtacga ctctggggct ggcagattta aacaaggccg agtttggagt
    26041 agctagattc actgactatc ctcgtatatg gtggcatgca agagaaggtg ccatcttccc
    26101 taataaagaa gatattgccc gtgcaacagg ggctgatata cgagctatgg aagaaggtat
    26161 acccgttgga cagcgacatc caaatccaga ggatgtggta atcgatatcg aaagcaatgg
    26221 ctcaccacat cataacccat caaattatgt tgataccttt gatataatcc aagaaacaag
    26281 agtataagct ttaacttgtt tattttaaaa taatagctga aatggatgac agctattatt
    26341 aataagaatc aatacatctt ttcctaaaaa cctcacaaaa gagcctgttt aagattccgt
    26401 gtaaatgcct tttctcagaa gtgaccgtcc aggcggtcac cgaactcgat aataaagcgg
    26461 ctcattgcca ttcgccagtc cttcaacggc atcgtccatt tctgtgaagc cgcctggatt
    26521 gccagccaca ccacttttta ccaactccat ccatgcaaca aatccactac actggataaa
    26581 attactaaat tttacttaat accacactaa taagatcttg ctttccgcca tgataagatt
    26641 gcattatttg gctttcactc catgaacggt cagaaacatt ataagatgat atatctattg
    26701 gattaaattc attccttaca cggtcatata agacatttgt ctctgttgta tcgataaaaa
    26761 gaattccttt ttgctccagc ctgtcaaaca tatcgtaaat agcatgctca gcctgtactg
    26821 gcaatgacga aatattaaaa agcgactccc cgtttatttt attcattcta ataccaataa
    26881 tatcgccatt atcgccatat attttttctg cactcccggc accataatat tggttgaagc
    26941 aacgaacttc gtttgttacc tcttcattgc tttgagatgt agtaaacatc ttcaggactt
    27001 ttgttgcatc ctcagcatct tcatatacta cagcgttacc ccctttgcca ataacattac
    27061 cagctagccg ctgcatccct tacagaggat aaaatacgac tgtcaggaga agtcaggttt
    27121 ctggttaaag aattccatga acaccccaaa tttacagaag atggtgatag catacatttc
    27181 aaccttcaaa atgaatcaat ctttactttc ttaacaaaca tcaccatgac atgacaacaa
    27241 aaaccggagc cggactccgg ttttttgtga acccgtcggc tatttcatcc cgccaacatt
    27301 ttcccacacc ccgtcagcac gcaggatttg ca
//
LOCUS       DAZFZC010000072        26900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFZC010000072 DAZFZC010000000
VERSION     DAZFZC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.072"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            66..797
                     /gene="artJ"
                     /locus_tag="ACHKXP_003322"
     CDS             66..797
                     /gene="artJ"
                     /locus_tag="ACHKXP_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO7648267.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(847..945)
                     /locus_tag="ACHKXP_003323"
                     /pseudo
     CDS             complement(847..945)
                     /locus_tag="ACHKXP_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(996..2123)
                     /gene="rlmC"
                     /locus_tag="ACHKXP_003324"
     CDS             complement(996..2123)
                     /gene="rlmC"
                     /locus_tag="ACHKXP_003324"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO7648268.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPQQLSAKTADLKNLLADFPVVEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2164..2652)
                     /gene="ybjO"
                     /locus_tag="ACHKXP_003325"
     CDS             complement(2164..2652)
                     /gene="ybjO"
                     /locus_tag="ACHKXP_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO7648269.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2712..3557)
                     /gene="potI"
                     /locus_tag="ACHKXP_003326"
     CDS             complement(2712..3557)
                     /gene="potI"
                     /locus_tag="ACHKXP_003326"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO7648270.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3554..4507)
                     /gene="potH"
                     /locus_tag="ACHKXP_003327"
     CDS             complement(3554..4507)
                     /gene="potH"
                     /locus_tag="ACHKXP_003327"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO7648271.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFNVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4517..5650)
                     /gene="potG"
                     /locus_tag="ACHKXP_003328"
     CDS             complement(4517..5650)
                     /gene="potG"
                     /locus_tag="ACHKXP_003328"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO7648272.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5745..6857)
                     /gene="potF"
                     /locus_tag="ACHKXP_003329"
     CDS             complement(5745..6857)
                     /gene="potF"
                     /locus_tag="ACHKXP_003329"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO7648273.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRANLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7208..7684)
                     /gene="ybjN"
                     /locus_tag="ACHKXP_003330"
     CDS             complement(7208..7684)
                     /gene="ybjN"
                     /locus_tag="ACHKXP_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO7648274.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7772..8674)
                     /gene="rimK"
                     /locus_tag="ACHKXP_003331"
     CDS             complement(7772..8674)
                     /gene="rimK"
                     /locus_tag="ACHKXP_003331"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO7648275.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8735..9457)
                     /gene="nfsA"
                     /locus_tag="ACHKXP_003332"
     CDS             complement(8735..9457)
                     /gene="nfsA"
                     /locus_tag="ACHKXP_003332"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO7648276.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9441..9728)
                     /gene="ybjC"
                     /locus_tag="ACHKXP_003333"
     CDS             complement(9441..9728)
                     /gene="ybjC"
                     /locus_tag="ACHKXP_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO7648277.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9888..10145
                     /gene="grxA"
                     /locus_tag="ACHKXP_003334"
     CDS             9888..10145
                     /gene="grxA"
                     /locus_tag="ACHKXP_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO7648278.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNEHDDFQYHYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10175..10552)
                     /gene="ybjM"
                     /locus_tag="ACHKXP_003335"
     CDS             complement(10175..10552)
                     /gene="ybjM"
                     /locus_tag="ACHKXP_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO7648279.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10822..12507
                     /gene="ybjL"
                     /locus_tag="ACHKXP_003336"
     CDS             10822..12507
                     /gene="ybjL"
                     /locus_tag="ACHKXP_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO7648280.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12682..13218)
                     /gene="rcdA"
                     /locus_tag="ACHKXP_003337"
     CDS             complement(12682..13218)
                     /gene="rcdA"
                     /locus_tag="ACHKXP_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO7648281.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13302..14510
                     /gene="ybjJ"
                     /locus_tag="ACHKXP_003338"
     CDS             13302..14510
                     /gene="ybjJ"
                     /locus_tag="ACHKXP_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648282.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14510..15325
                     /gene="ybjI"
                     /locus_tag="ACHKXP_003339"
     CDS             14510..15325
                     /gene="ybjI"
                     /locus_tag="ACHKXP_003339"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO7648283.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVSVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15411..15695
                     /gene="ybjH"
                     /locus_tag="ACHKXP_003340"
     CDS             15411..15695
                     /gene="ybjH"
                     /locus_tag="ACHKXP_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO7648284.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15736..16968)
                     /gene="mdfA"
                     /locus_tag="ACHKXP_003341"
     CDS             complement(15736..16968)
                     /gene="mdfA"
                     /locus_tag="ACHKXP_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO7648285.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17253..17849
                     /gene="ybjG"
                     /locus_tag="ACHKXP_003342"
     CDS             17253..17849
                     /gene="ybjG"
                     /locus_tag="ACHKXP_003342"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7648286.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAVV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            17907..18665
                     /gene="deoR"
                     /locus_tag="ACHKXP_003343"
     CDS             17907..18665
                     /gene="deoR"
                     /locus_tag="ACHKXP_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO7648287.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18712..19914)
                     /gene="dacC"
                     /locus_tag="ACHKXP_003344"
     CDS             complement(18712..19914)
                     /gene="dacC"
                     /locus_tag="ACHKXP_003344"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7648288.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRVWDFVMMKFH
                     QWFGSWFS"
     gene            20161..20787
                     /gene="gstB"
                     /locus_tag="ACHKXP_003345"
     CDS             20161..20787
                     /gene="gstB"
                     /locus_tag="ACHKXP_003345"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO7648289.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20784..21899)
                     /gene="yliI"
                     /locus_tag="ACHKXP_003346"
     CDS             complement(20784..21899)
                     /gene="yliI"
                     /locus_tag="ACHKXP_003346"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO7648290.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKPDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(22010..22393)
                     /gene="bssR"
                     /locus_tag="ACHKXP_003347"
     CDS             complement(22010..22393)
                     /gene="bssR"
                     /locus_tag="ACHKXP_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO7648291.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22606..23931
                     /gene="rimO"
                     /locus_tag="ACHKXP_003348"
     CDS             22606..23931
                     /gene="rimO"
                     /locus_tag="ACHKXP_003348"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO7648292.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23978..25306)
                     /gene="dgcI"
                     /locus_tag="ACHKXP_003349"
     CDS             complement(23978..25306)
                     /gene="dgcI"
                     /locus_tag="ACHKXP_003349"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7648293.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLVDERLYKSKKNGRAQISWQ"
     gene            complement(25314..>26900)
                     /locus_tag="ACHKXP_003350"
     CDS             complement(25314..>26900)
                     /locus_tag="ACHKXP_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO7648294.1"
                     /translation="YIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVL
                     LVVYLMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEM
                     KDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVD
                     ERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGY
                     RNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWI
                     ARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIG
                     FINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHECFTTIMKENG
                     LKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDS
                     IKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGY
                     LYQKPMPFSAWDKSGKLVKE"
BASE COUNT         6830 a         6968 c         6481 g         6621 t
ORIGIN      
        1 ggcattgtta tcccatgccg cagacacggc caaaaatcat aagattgaca gacgggagtt
       61 ccatcatgaa aaagttagtt cttgccgctt tacttgcttc ctttactttc ggtgcttctg
      121 ccgcagagaa aatcaatttt ggcgtttcag ccacctatcc accctttgaa tctataggtg
      181 ctaataatga gattgtcggc tttgatatcg atctggcaaa agccttgtgc aaacaaatgc
      241 aggcagaatg tacttttact aatcacgcgt tcgacagcct gatcccgtcc ctgaaattca
      301 gaaaatatga cgccgtaatc tccggtatgg atatcacccc ggagcgtagc aaacaggtat
      361 cgtttaccac gccctactat gaaaactcag ccgtcgtgat tgccaaaaaa gatacctaca
      421 aaacgtttgc cgatctgaaa ggcaaacgta ttgggatgga aaacggtact acgcaccaga
      481 aatatattca ggatcagcac ccggaagtga aaactgtctc ttatgacagt tatcagaatg
      541 cctttatcga tctgaaaaat ggtcgtattg atggggtatt tggtgacaca gcggtggtaa
      601 acgaatggct gaaaaccaat ccacaacttg gtgttgctac tgagaaagtg accgatccgc
      661 aatattttgg caccggcctg ggcatcgctg tacgtccgga taacaaagcc ctgctggaaa
      721 aactgaataa cgcgctggca gcaattaaag ctgacggtac ttatcaaaaa atcagtgacc
      781 agtggttccc acagtaagat tcagaaagcc gcaaccgcgg cttgagactg atgacaaagg
      841 taaaattgcc tgatgcgcta cgcttatcag gcctacatgg cccatgcaat gtattgaatt
      901 tgcacgcttt tgtaggccgg ataaggcgtt tacgccgcat ccggcataaa caacgcgcac
      961 tttgtcagca atctgaagcc gcacatgcgg cttttttatt gcttcaccag cagcgtcagc
     1021 acttcatagt gcgcggtatg cgggaacata tcgaaaagct gcacccgttc aatacgaaac
     1081 ccaggcagtt cgcggatatc tttcgccata gtttgggcgt tacagctgga gtagatgata
     1141 aaacgcggtg ccatcgttga gagataatca cacagcggtt taccaatgcc gcggcgcggc
     1201 gggttaacca gcaccagctc cggcacatcc ccctgagcgg tggcaaactg agtggagtcc
     1261 agcgcctgaa attgcaaacg cgttaagccc agttcagcgg ctgactgctt tgcacaggca
     1321 atggcctctg atgcaatttc gatcccggtt aactgcatgt caggcgtcgc gcagtgtaaa
     1381 ccaaagcccc ccacaccgca gaacagatcc cacatatgtt taaccggcag ctgtcgtacc
     1441 cagtcgcgcg cggtggcgta caactggctg gcgaccgccg gattagtctg gaagaaactt
     1501 tgcggacgga tccacagcgg tacgtcatta aaacgctccg ccagtgcctg ttgttcggtc
     1561 aggtagatct ccgtctcccc ttccataatc gccatatgta ccggctgaat attgacggta
     1621 ataactttca gctgcggtag ttgttcgtgt aaccacggca gcgccttacg cagttgcgcc
     1681 agcttggtat cagaacgcag tacaaagcgc agcatcatgc cgccatcact ctggctttca
     1741 gtcagcagaa tgtatttcag ttcgccacgc ttacgcgcca cgttataagg cgttagcccc
     1801 gctcgggcga taaacggttt tagcgccgca aaaacgggcg caaatgaggc aggataaagc
     1861 gggcagtcac aaaggtcttc cggtgtgccg tcgcgatgca gcataccgag cagtggtttt
     1921 tcaacgctac cactcaccac cattttggct ttattacgaa acccttgttc cgggcctgac
     1981 accggcgcgc accattccac gaccggaaaa tcggcgagca gatttttaag atcggcggtt
     2041 ttagcggaga gttgctgtgg aatcggctgc gttatccact gacaggaacg acagcgaccc
     2101 gcgtcgtaaa gtgcgcactg catacattga ccttcacatc atcaggggcg acgattatac
     2161 acattattgc aactggaaga accgccgact ggtcgaggga acgaacagca gcatcaggat
     2221 gagcatatcc ggcagcttct gcagcatcag gctatggaag atttcacgtt ttgattcacc
     2281 gggaatgctg aacagctccg gataaccata ccccagcgaa gccgcccaca agtaacttgc
     2341 ggccgtgatt tgtgtcaaca gatacagcca tcgcgcccag cgacgccctt tcaccagtga
     2401 aaacgcacac cagatctcaa tgaaaaccag caccagactg cttaaaaaga ccagcgttaa
     2461 actccaggtt tgtacgctgc gatgaatgaa ctcgccaata ccgcgcacgc ccagcgtatt
     2521 gaaaatcatc agcacgtcga ggccacggat cataataatg gcgagcgccg ccacctgcac
     2581 cagcgcaggc acattcaggc gagcatgaga tgaagatgtt ttcttaaaaa atcccaacgt
     2641 ttcgtcttcc atgaaaacaa tgccgcgaca tgcgcggcat tatgtagcca ggttggcaaa
     2701 ttttagtgtc ttcagccacg tcttgcacgc tggatgtcgc gtatccgctg tttttctgcg
     2761 cgagccatca gataccaggc gataaatccg acaattccga ccgcgccgag gatcaatgtt
     2821 gccagggcgt tgatttccgg atttaccccc atccgcacgc tggagaagac cagcatcggt
     2881 aacgtggtgg ctcccggccc ggaaacaaag ctggcgatca ccagatcatc aagcgacaaa
     2941 gtaaaagcca gtaaccagcc agaaataatc gcgggcatga tcatcggtag cgtaatgaca
     3001 aaaaacactt tcagcggcgt cgcaccgaga tccatcgctg cttcttctat cgagcgatcc
     3061 agttcccgca gacgcgacga aataacgact gccacataag ccgtacagaa agtgacatgc
     3121 gccagccaga tggtgagcat accgcggtcc gcaggccagc caatggcgtg tgcaagcgcg
     3181 acaaacagta gtagcaacga cagcccggtg atcacatccg gcatcaccag cggcgcggtg
     3241 atcataaagg caaaaccatt tgatccgcga aacctgccaa agcgcaccag cacgaccgct
     3301 gcaatagtcc cgaggattgc cgccgccgtt gccgcacagg cagcaattgt caggcttaaa
     3361 ccaaccgcac tcatcatcgc gtcatcgcgc aataactcac cataccagcg cgttgaccag
     3421 ccggcccaca ccgtcaccag cttcgagctg ttaaacgaat agataaccag catcagcatt
     3481 ggcgcgtaga gaaaggtaaa gcccagcagc aaaatcacaa tccgccaggg tgaacgaact
     3541 accggtaaat tattcatccg tgttctccca cgcttttttg ctggtgtttg tggaaccaca
     3601 ttatcggcac aattagcagc agcaacatga tgatcgctac cgccgaggcc accggccagt
     3661 cgcggttatt gaaaaactct tgccatagca cgcgcccgat catgatgctg tccgggccac
     3721 cgagcagttc cgggatcaca aactcgccca cagccgggat aaacaccagc atcgatccgg
     3781 caataatccc acctttagtc agcggcacga tcacattaaa gaacgttttc agcggtcgtg
     3841 caccgagatc cagcgctgct tccaccagcg aataatcaat acgaatcaac gcggtataaa
     3901 tcggcagtac cataaacggc acgtaagcgt aaacaatgcc gatataaacg gccagattgg
     3961 tatgcagaat ggtcagcggt tgatcgataa cccccagcca cagcagaaaa ttattcagca
     4021 caccgttgtt ttttaatatt cccatccagg cgtagacacg aatcagaaac gaggtccacg
     4081 acggcaggat caccagtagt aataagatat tacgggtcga aggcttactg tgcgccactg
     4141 cccacgccag cggatagccg atcagtaaac agcaaaatgt cgaaatcgca gccacctgta
     4201 gcgactggag ataagcatcg aaatagagcg gatcgtcggt cagttgcagg aaattaccga
     4261 gattaagagt gatggacagt tgcccgtcag cccactccat cagttcggta taaggcggaa
     4321 tggcgcgcgc catctccgcc agacttattt taaagacgat cagaaatggc agcagaaaca
     4381 gcaagatcaa ccagatatat ggcaacgcaa tgaccagttt gcgcccatgc ttcatttgca
     4441 gctgcgacaa ccacagctta aatccgcccg gcggtttcga ctgggcagca ggttcaagtg
     4501 tattcatctt tgctccttaa accgtcagca ccacacagct gtccacttcc cagcacaaac
     4561 gcacttcgtc gccccaggtc ggtaaccctt tacgatgacg atgggcgttt tgtagctggg
     4621 cgctaatcat ctgcccactt ttcagacgaa cgtgatacac cgaaagatcg ccgagatagg
     4681 caatatgtat cacctccccc accgcgaagt tacaaccatt ggcgggcggc tcttcgcaaa
     4741 gcatgatttt ttccgggcgc agagccacat gtaccggcac gttatcgacc accgaggcat
     4801 ccgcgtcgac tttcagtgga tgcaccagcc ccggcgaatc aagcaccagg ccatcttcct
     4861 gacgctcttt gagtacgcct tcaaagacgt ttaccgagcc aataaattcg gcgctatagc
     4921 gggtagtcgg atgctcgtag atctcttccg gttcgccaat ctggacaaat ttcccacgat
     4981 tcataatggc gatgcgcccc gccatggtca tcgcctcttc ctgatcgtga gtaaccatca
     5041 cgcaggtcac accgacgcgc tccagaatat ccaccacttc aagctgcatc cggtcacgca
     5101 attttttatc cagcgcgccc atcggttcgt cgagcagcag aagttttggg cgcttggcaa
     5161 ggcttcgggc cagcgccaca cgctgtctct gaccgccgga aagctgatgc ggtttgcgtt
     5221 tggcgaactc ctgcatatgc accagcccaa gcatctcgtt gacccggctg gcaatttccg
     5281 ctttcggtag tttgtcctgt ttcaggccaa aggcgatgtt ctgctccaca gtcatatggg
     5341 gaaacaatgc gtaagactga aacatcatat tgatggggcg tagataaggt ggcacctgtg
     5401 acaaatcgac gccatcaagc ataatctgtc cggcagaggg ttgttcaaaa cctgccagca
     5461 tacgcagcag cgtggattta ccacagccgg aagcgcccag cagcgcgaag atttcacctt
     5521 tgtagatagt caggctgaca tcatccaccg catgttgacc atcgtaggat ttggtgaggt
     5581 tgcggatttc taatagcggc gtcagcgcct tacgggtttt cgcctgcggg cgagggatag
     5641 cgtcattcac ggctgctctc cggcattgaa tgtgtgcaag cgcaccatga tggtgcgaat
     5701 tgccggcggg tgtggtgcgc ccgcccggca tctaaggctg cggattattt tccgctcttc
     5761 actttggtcc acgcgcgggt gcgcacacgg tcgattttcg ggtcctgcac tttcagagtg
     5821 aacagattcg cacgaacatc cgcaggcgga taaatacccg ggttctcacg gacttccgca
     5881 ctcaccagcg gcgtggctgc tttattggcg ttggcataga acacatggtc ggaaatatgc
     5941 gctactacat ccgggcgcag caggtaattc aggaactgat aggcttcgtc tttgtttttg
     6001 gcatccgcag gcatggcgaa tacatcaaag aacgccatcg ccccttcttt tggaatcgag
     6061 aacgagacat tcactccatt cttcgcttcc ttcgcgcggt ttgacgcctg ccagacatca
     6121 cctgcccagc cgatagcgac gcaaatatcg ccgtttgcca ggtcgttaat gtattgagat
     6181 gaatggaaat aacgaatgtt cgggcgcagc tttaacagca gatctgttgc cggtccggtg
     6241 taatcatccg ctttagtgct gttgggatct ttgccgagat aattcaacac ggtagcaaaa
     6301 acttcttctg gcgcatccag gaaagagaca ccgcagcttt tcagtttttc cagattttca
     6361 ggtttgagga tcaagtccca gctatcgacg ggcgcgtttt cgcccagcac cgctttaact
     6421 ttatcaacgt tatagccaat cccggtcgtc gcccacatat agggcatagc aaatttattg
     6481 tcgggatcgt gtttggcgac cagcttcagc agttccgggt cgagattctt ccactccggc
     6541 aatttgcttt tgtccagcgg ctggaaaact cccgcagtca actggcgctc cagaaagctg
     6601 gcagatggaa ccaccagatc aaagccggta ctcccggcca ttaatttgcc ttccagtact
     6661 tcgttagagt cgaaaacatc gtagacgact ttaataccgg tttctttttc aaaattggcc
     6721 accgtgtccg gggcgatata atcagaccag ttataaatgt ggagtgtttt ttgttcagcc
     6781 gcgagcgtgc cgacagagac ggccatcaga gcacccgcaa ccagacccga tagccatttt
     6841 ttatttaagg cggtcatatc tctttccttc tgaaagttcg ttaacaaacg aacaaaagtt
     6901 gtcacactga aagatatgca ataaacgtgc atatttttta tcggcgcggt gtaagaaaag
     6961 agaactttct cccggccagc actgctcgct atataaaagc atcgcaagaa taactttagc
     7021 caggatttga gactatagct cggattaaaa aacggtattt aacaaatttt tatgcatatt
     7081 tgctctatgt gataaagcca aatcgacaca agagaagtga aataatcttt aagaaaaggt
     7141 taaaaacagc agataatttt gcagcgcgca gccactgcac cagcgactgc gtgctcgaaa
     7201 ggagttatca gtggaggaaa tagttttcgg taacgttgtt ttgcccttca tcatcagtcg
     7261 gtaacagcag ttgatgggca ttagcttcaa gaatgaccat cgaaatctgc tcttcgctct
     7321 gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg cataacggat aaagactggc
     7381 aaaccaccag ctttggcaga ttatcatcct gcatatcaag aaatgctttc acggtcagcg
     7441 aactggcatt gatggcagat aaatccgccg ccagcggcaa tacggctgaa ggtcggactt
     7501 ccgccatggc agaaaacagg atcgtgttat cgatcagatc gattttggca tcaaagacac
     7561 cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca gttatcacat tcaaaaaaac
     7621 tcatccccag gtcatcgagc cattgacgca gcgtatccag accaggaacg accagcgatg
     7681 tcataatttt gtacaacctc tttcgataaa aagaccggca cagcttacgc aaaaagcgca
     7741 ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc cgttttcagg caatattctg
     7801 tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc gatgtcgata ccggtggttt
     7861 tttctattcc ttccagcccc ggcgacgcat tcacctccat caccaacggc ccgcgattag
     7921 cacgcagaat atccacacca gcaacgtcca gcgccatcgt tcgcgcggct tttatcgcga
     7981 tttcacgctc ctgtggtgtg atactggcga cgcttgccgc gccgccacga tgcaaattgg
     8041 aacgaaaatc gccctctttc gcccgccgtt caatcgcagc gaccacttca tcgccaacaa
     8101 ccagacagcg gatatcgcac ccttgcgcct ctttgatata ttcctgcacc agaatatgcg
     8161 cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc ctgacgcgtc tccgccagca
     8221 cgacgccaat tccctgcgtg ccttcaacca acttgaccac cagcggcgca ccaccgacca
     8281 tgtcgattaa atcgctggta tcatccggcg aatgcgcaat gcccgtgaca ggcaggtcga
     8341 tgccctgacg cgccagcagt tgcatggaac gcaatttgtc acgcgcccgg gcaatggcga
     8401 ccgactcatt gagcggatag ctccccagca tctcgaactg gcgcagtgcc gccgtcccat
     8461 aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc aaaatggggt aacttgcggc
     8521 ctttgtagtg aatagaagac gccgcaggat ttatgttcat gtagcaagaa agcggatcaa
     8581 gaatttcaac caggtgaccg cgctgtatag cggcttcacg cagccgctta cacgaataga
     8641 gcgttccatc ccgggacaat atggcaattt tcaccctgca cctctctgtc agacctggtc
     8701 aaaagcccgc gtatcataca tcgacgtggc ggttttagcg cgtcgcccaa ccctgtttgt
     8761 gcaaataatc cagaataaat gggcggcttt ctttaatgat tgttcggcgg atatgatcgc
     8821 tccaggtatc ccggcgatta ttgctgccac gggtgaggta atattccgcc agttgctcgt
     8881 catactgcgc cagtgcgcct ttatccagcg gttgatagct gttttcatgc accaaaatgg
     8941 aggccggtaa acgcggctta agatccggat tatccgcagg ccagccaagg cacagcccaa
     9001 acagcggcag aacatgctgc ggtaatttaa gcagtttcgt caccgcttca atattattgc
     9061 gcaggccgcc gatatatacc ccgcccaatc ccagcgattc cgctgcgatt aatgcattct
     9121 gcgccatcat tgccgtatca acgacaccga gcaacagttg ttccgccagg ccgagctgag
     9181 catccggaca gatctgtaaa tggcggttaa agtcggcaca gaacacccag aactccgccg
     9241 cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag ttcttcacgt aacgctttgt
     9301 cggtaatgcg aataatgcta ctgcactgca aaaaactgga actggacgtc gcacgggcgc
     9361 tgttaataat cgcctcacgc tgcgcttcgg aaatgggttc atcagtgaaa tggcgaatgg
     9421 agcgatggcc acaaataagt tcaatggttg gcgtcattat ctttttctct ttctgaacgt
     9481 gaatattgcg gtggacggtt catcagctgt ggggcaagac gttttgccac ctgaagaata
     9541 accaccaccg cagcgggaag catgagcaaa acaccgagaa aaatcatcag aatctgcact
     9601 tctggccgag aaaatggctc aggcagcgac agggagtcgc ttaccgacag cagcgccacc
     9661 gccagtagca tcattccgat aaattccagt atcaacacgc ctttaggcaa tttaccgatc
     9721 gcgcgcatac gcttccctct gcaaagtgag ccttcagtct aaaacttttc actgtattgt
     9781 gtttaacagt tatagctttt agcaattaat gcaacaggtt aaacctactt tcagcgaata
     9841 cattttagcg tgatcattac aggcataaat ctatgaggag agaaataatg caaaccgtta
     9901 tttttggtcg tccgggttgc ccttactgtg tgcgtgcaaa agatctggct gagaaattga
     9961 gcaatgaaca cgatgatttt cagtatcact atgtagatat tcgtgcggaa gggatcacta
    10021 aagaagatct acaacaaaag gcaggtaaac ccgtagaaac cgtgccgcag atttttgtcg
    10081 atcagcaaca tatcggcggc tataccgatt ttgctgcatg ggtgaaagaa aatctggacg
    10141 cctgatcgtc tgacaagccc tcgcgttgag ggctttactg attttttctg tgctgtggtt
    10201 taaacaaact actgataaat aagaaacaca gtgcccccag cgcacaccag aacaccgcac
    10261 ttaataacca tgccagctct tgccagaatg agcgcgtcgg tgaaaaaaac agccgcataa
    10321 tgagcatcga acagggtgcc gccagcattg cgccaaacag aggtttcagg acttctctac
    10381 gctgtgaaaa gaagctggcg actgctccag gaagaatgaa aaatagcaag ccgatttcag
    10441 gatgcccggc agcccgaaaa gcgcctttca tgtgcgtcgc cagaaaaagg cacaccacaa
    10501 tgaagaggac aaaacagcag attgcccccg cccaacgttg tttatgtttc actcgttcct
    10561 cctgacactg cgtctatcga acacattttt cgccagtgtg gcgttcagta agataaagcc
    10621 gcttcgcatt ccatgctaat ataggccaac gcaattcata tagccgttga tacctaatgt
    10681 gattacacta gtaaaatata ttgttacttt actatcgttt aggtgcgctg aatgaatctg
    10741 cgccctgaat tctggtaaaa aacattatcg taaattacca tttctttcaa cagcttacta
    10801 gtaaacaaga agttagcctc cgtgaatata aacgtcgccg aattgttaaa tgggaattac
    10861 attctgttat tatttgtggt cctcgcgctt gggctatgtc tcggaaagtt acgacttggt
    10921 tcgatccaac tgggtaattc cattggcgtt ttagtcgtat cgctgttatt aggccaacaa
    10981 catttcagca ttaacaccga tgcgcttaat cttggcttta tgctgtttat tttctgtgtt
    11041 ggggttgaag ccggaccgaa ctttttttcc attttttttc gcgatgggaa aaattaccta
    11101 atgttagcac tggtgatggt tggcagtgcg ctggtgatcg ccttagggtt aggtaagctg
    11161 tttggctggg atattggcct gacggccggt atgttagcag gctctatgac gtcgacaccg
    11221 gttctggtcg gtgctggcga tacactgcgt cattccggca tggaaagcag gcagctctca
    11281 ctggcactgg ataatctgag cctcgggtat gccttaacct atttaatcgg tctggtgagt
    11341 ttgattgttg gtgcgcgtta cttgccgaaa ttgcagcatc aggacttaca gaccagcgcc
    11401 cagcaaatcg cccgcgaacg tggcctggac actgatgcca accgtaaggt ttatttaccg
    11461 gtgatccgcg cctatcgcgt cggcccggag ctggtggcct ggaccgacgg caaaaatctg
    11521 cgtgaactgg gtatttatcg acaaaccggc tgctacattg aacgtattcg acgtaacggg
    11581 attctggcaa atccagacgg tgatgccgtg ctacaaatgg gcgatgaaat agcgttggta
    11641 ggctatcccg acgcccatgc ccgactcgat cccagcttcc gtaacggtaa agaagttttc
    11701 gatcgtgacc ttctcgacat gcgtatcgtc actgaagaag tggtcgttaa aaaccataac
    11761 gctgtaggta aacgtctcgc acaactgaag ttgaccgatc acggttgctt ccttaaccgc
    11821 gtcattcgta gccagattga gatgccgata gatgacaacg tcgtgcttaa caaaggtgac
    11881 gttttacaag tcagcggtga tgcccgtcgc gtaaaaacca tcgccgatcg catcggcttt
    11941 atctcgattc acagccaggt cactgacctg ctggcatttt gcgccttctt tgttattggg
    12001 ctgatgatcg ggatgatcac cttccagttc agcacattca gtttcggcat ggggaacgct
    12061 gccgggttgt tattcgccgg aattatgctg ggctttatgc gtgctaacca cccgaccttc
    12121 ggttacattc cgcagggtgc attaagcatg gtgaaagagt tcggcttgat ggtgtttatg
    12181 gcaggcgttg gtctgagcgc cggtagcggt attaataacg gtctgggcgc gattggcggt
    12241 cagatgttga ttgccggatt gattgtcagt ctggtgcccg tggttatctg tttcttgttc
    12301 ggtgcttatg tattgcgaat gaaccgcgcg ctgttgttcg gcgcaatgat gggcgcacgt
    12361 acctgcgcgc cggcaatgga gatcatcagt gatacagctc gcagtaacat cccggcgctg
    12421 ggctatgcgg gcacctatgc aatcgccaac gtcctgctga cgctggcagg gacaatcatc
    12481 gtcatggtat ggccaggatt aggataaaac tgaagttgcc ctgaaaatga aatttttttg
    12541 cacaaccgca gaacttttcc gcagggcatc agtcttaatt agtgccactg cttttctttg
    12601 atgtccccat tttgtggagc ccatcaaccc cgccatttcg gttcaaggtt gatgggtttt
    12661 ttgttatcta aaacttatct actaccctgc aaccctctca accatcctca aaatctcctc
    12721 gcgcgatagc ggcttacggt cggtgacaaa atgcagcgtc atcccctcaa taaacgcatc
    12781 aagcgcgcgg gcggttccgg gttcaaacca ttgttcgagc gtttgctgac tgcgctgcat
    12841 ccagttttgc attaccgttt ttaatagcgg ttttcggcta gccagcgcgt agagctggta
    12901 catcagctcc atgttatccg gcgttgcaac ctgtgagctg tagatcatat cggtgatagc
    12961 ctggcatgcg cccggagcat cactaacatc gctaaaaaat gcctgatatt gccgggacat
    13021 gatctcagta aaactgctga acgcctccag taacaactca tcaattcctg aaaaatagta
    13081 ggtcatcgat cccaacggta ccccggcaag ggtagcaatt ttgcggtgcg taacagcatg
    13141 tattccgtaa agtttcaccg cctccagcgt ggcctggata attttttctc gccgttgcgg
    13201 atcgttagcg cgacgcatgt catttcctct ctctgcaatt gtgtacaaat gtacacaacc
    13261 ttgctaatgt tgtcttccct cttcttattt tgacgtgatc tatgaccgta aattcttcac
    13321 gtaatgcatt gaaacgccga acctgggcgc tgtttatgtt cttctttttg ccaggcctgt
    13381 taatggcgtc ctgggcaacc cgtacgcctg ctatccgcga tattctctct gtctcgatcg
    13441 ctgaaatggg cggtgttctc tttggtctgt cgatcggttc gatgagcggt attctctgct
    13501 cggcgtggtt agtgaaacgc tttgggacac gtaatgtcat cctggtcacg atgtcctgcg
    13561 cattgatcgg gatgatgata ttaagtctgg cactctggct gacatcgccc ctgctctttg
    13621 ccgttggtct cggcgtcttt ggggcaagtt ttggttctgc ggaagtggcg ataaacgttg
    13681 aaggtgctgc cgttgagcga gaaatgaata agacggtttt gccgatgatg cacggttttt
    13741 atagcctggg cacgctggca ggcgcaggtg tcgggatggc actgacggcc tttggcgttc
    13801 cggcaacggt gcacatttta ttggcggcgc tggtaggtat cgcacctatt tatatcgcca
    13861 ttcaggcaat ccctgacggt acgggcaaaa atgctgccga tggcacccag catggcgaaa
    13921 aaggcgtacc tttttatcgc gatatccagt tgctgctgat aggtgttgtg gtgctggcga
    13981 tggcctttgc cgaaggttct gccaacgact ggttaccctt attaatggtt gacggtcacg
    14041 gttttagccc tacttccggc tcgctgattt atgccggttt taccctgggg atgaccgttg
    14101 gacgcttcac cggcggttgg ttcatcgacc gttacagtcg cgttgccgtg gttcgggcca
    14161 gtgcactaat gggggcgttg ggtattgggc tgattatttt tgtcgatagc gcctgggtcg
    14221 ctggggtgtc tgttgtactc tggggactgg gtgcctcgct gggcttcccg ctgaccattt
    14281 ctgccgccag cgataccggc cccgatgcac caacccgcgt cagtgtggta gcaacgaccg
    14341 gttatctggc tttcctcgtc gggccgccgc tgctgggcta tctcggcgaa cattatggat
    14401 tacgtagtgc aatgctggtt gtactggcgc tggttattct cgcggctatt gtcgcgaaag
    14461 ccgtcgccaa acccgatacc aaaacgcaga cggcgatgga gaatagttga tgagcattaa
    14521 attaattgcg gtagacatgg atggtacttt cttaagcgat caaaaaacct ataaccgtga
    14581 gcggtttatg gctcagtatc agcaaatgaa agcacaagga attcgctttg tagtcgccag
    14641 cgggaatcaa tattatcagt tgatctcttt cttccctgaa attgctaatg aaattgcctt
    14701 tgtggctgaa aacggcggct gggtagtgag cgaaggcaaa gatgttttta atggcgagct
    14761 atcgaaggat gcgtttgcta ctgtcgtgga acatttgctg acgcgcccgg aagtggaaat
    14821 tattgcctgc ggaaaaaata gtgcctatac actcaaaaaa tatgacgatg ccatgaaaac
    14881 ggtggcggaa atgtattatc accgtctgga atacgtcgat aactttgaca acttagagga
    14941 tatcttcttt aagtttggcc tgaatctttc cgatgaactg attccacaag tacaaaaagc
    15001 attacatgag gccatcggcg atattatggt gtcggtccac accggcaacg gcagcatcga
    15061 tctgattatc cccggcgtac ataaagccaa tggccttcgc caactgcaga aattatgggg
    15121 aatagacgac agcgaagtgg tggtctttgg cgatggcggt aacgatattg agatgctgcg
    15181 tcaggcaggc tttagttttg caatggaaaa tgccggtagc gcggtcgtcg cagcggcaaa
    15241 ataccgcgca ggctccaata accgtgaagg cgtactggat gtgatcgata aagttcttaa
    15301 acacgaagcg ccatttgacc aataaaaccg gcagcacgcc cggcaacaca atgcctgaat
    15361 gaaggctgca taattcgcgg aaattaaccc gctgcattat ttcaggcgaa atgattatga
    15421 aaaattgtct actgttgggc gcgcttttaa tgggctttac tggtgtggcg atggcgcaaa
    15481 gtgtcaccgt cgatgtgccg agtggataca aagtggttgt ggttcctgat tcagtcagcg
    15541 ttccacaagc ggtcagcgtc gcgactgtgc cgcaaaccgt ctatgtggct cctgcgccag
    15601 ctccggctta ccgcccgcat ccttatgttc gccatcttgc cagcgtaggt gaagggatgg
    15661 ttatcgaaca ccagattgac gaccatcacc attaattcga gaatgcctga tcgcacaaag
    15721 cagtcaggca tttttttacc cttcgtgaga atttcccatc tgtttatctt ttaaaaagat
    15781 aaccatcagc gacagccaca aaattccgtt gacaaggttg aagagattaa acagtccgtt
    15841 gcccccgttc agccaggcat gtttgctgat ttcaatacca acggtaaaga tcagcatttg
    15901 cagcattccc atcgcggcag aaaccgtacc tttactcata tcgctggcaa acagggttaa
    15961 tcgcaccagt cccgcattcg ccagaccaat accgaaagca taaatactta acccggcagt
    16021 catccataaa tacgcgtgcg atgagataac cgttgccgca gcagcgacca atagaccaat
    16081 cataatcggc cagccgccca taataatcag cgaacgtacg gtgcggcgcg aggtcagacg
    16141 cgctaacagc aagttacccg caattaacgc cccgaaaata ggcacttgca gcaagccata
    16201 ttcatagctg ctcaactgct cgccggtaat gatgataatc ggcgactggg cgatccacgc
    16261 cagcaacggc agactaacga atcccagcgc cagcgccccc gccacaaagc ggccgttctt
    16321 cagcaccagc ttatagtcac gaccgagttc tttcagtgac agtttctcgc ctatacgcgt
    16381 ggcggtttca ggcatggctc gttgcagacc gaaaaaggag atcgctgcca atgcggcaaa
    16441 caaaacaaac atcccctccc agggcagcac atggatccac gccgcgccca ccagcggacc
    16501 aagtagcgga gcaatcagcg ccacgttcgc catcagcgcg gtgatcttga tacaaaccgc
    16561 ctcttcgaag gattcctgaa ttgcggcgta tcccacagcg ccaatgaaac agaggcttat
    16621 gccctgcaag aagcgcaaca gggtgaattg ttcaatgttt tgcgccagca atattgccag
    16681 acaggtgacg ataaaccaca ccactcccgc cagcatcacc ggacggcgac caatacgatc
    16741 cgacagcggc cccagcagcc attgtaaaaa catcccgccc gccagatacg cggtcatcga
    16801 agtaggaacc caatcaatgc ccgcctgata ttgttccacc acggccaaca taccgggttg
    16861 aatcatatcg ttgccgatat aggttgaaaa ttcgtaaagc accagacaga gagggaaaag
    16921 taacgcctga cgtccaagcc tggcaccgga agctaattta ttttgcatgc aatttcttcg
    16981 ccaataataa tcgcgcagag tttaataaaa gcgcagctaa cgagaaagcg aattttgtag
    17041 ctgaaaccac ggttaagcac attcttacat tattacgagt atagctacgc tttctttaag
    17101 ttttatttaa cctatgcccg ttacaatcac ccaccgtaaa caggccgctt gagggaaata
    17161 agacgatgcc gctttaccca gtttaacctg cactttattc tcaacgactt gcctgtattg
    17221 gctccctttt aatcactttg cgtcgggaag ttatgctgga aaatttgaat ctctctctat
    17281 tctctcttat taacgcgacg ccagactcgg ctccgtggat gatctcgttg gcgattttta
    17341 ttgctaaaga tttgattacc gtggtgccgt tgctggccgt ggtactttgg ttgtgggggc
    17401 ttacagcaca acggcaactg gtgataaaaa tcgctatcgc gctggcggtc agcctgtttg
    17461 tgtcctggac gatgggacat ctttttccgc acgaccgacc ctttgtcgaa aatatcggct
    17521 ataacttcct gcatcatgcg gcggatgact cattcccaag cgatcacggt acggtgattt
    17581 tcacctttgc actggcattt ttatgctggc atcgcctgtg gtccggctca cttttaatgg
    17641 tgctggccgt cgtcattgcc tggtcgcgcg tttatcttgg cgtccactgg ccgctggata
    17701 tgctcggtgg attgctggca ggtatgattg gctgccttag tgcccagatt atctggcaag
    17761 cgatggggca taaactctat caacgtctgc aatcgtggta tcgcgtctgt tttgcattac
    17821 cgatccgcaa aggctgggtg cgtgactgat ctctgctaaa aagtgtagta ttgagcggct
    17881 cgcttcaata actattcaga gggattatgg aaacacgtcg cgaagagcgt atcgggcagc
    17941 tgctgcaaga attaaaacgc agcgataagt tacatcttaa agacgccgcc gccctgcttg
    18001 gggtttcgga gatgacgatt cgtcgcgatc tgaacaacca cagtgcgccc gtcgttttgc
    18061 tcggcggcta tattgttctg gaaccgcgca gtgccagcca ttacctgtta agcgatcaaa
    18121 aatcccgcct ggtggaagaa aaacgccggg cggcaaaact ggctgcgacg ctggtagaac
    18181 ccgatcagac cctctttttt gactgtggca ccaccacgcc gtggattatt gaagcgattg
    18241 ataatgaaat cccttttacc gccgtttgtt attcgctaaa tacctttctg gcgctgaaag
    18301 agaaacccca ttgccgcgcg tttctttgcg gtggtgaatt tcacgccagc aacgccattt
    18361 tcaaacccat cgattttcag caaacgctga ataatttttg cccggatatc gctttttatt
    18421 ctgcggcggg cgtgcatgtc agtaaaggcg ctacctgttt taatcttgaa gagttgccgg
    18481 taaaacactg ggccatgtcg atggcgcaaa agcatgtgct ggttgtcgac cacagtaaat
    18541 ttggcaaggt gcgtccggcg cgcatgggtg acctgaaacg ctttgatatt gtggtgagcg
    18601 attgttgccc ggaagatgag tatgtgaagt acgcgcagac gcagcgcatt aagttgatgt
    18661 attaatgacg tataaccgga tgacgtttcg cgccatccgg ttatcagaag attaagagaa
    18721 ccagctgccg aaccactgat ggaatttcat catcacgaaa tcccacaccc gaccaaagaa
    18781 tccgccctct tccacatttt ccatcacgat cagcggacgc tgctcaatgg atttaccgtt
    18841 aagctggaaa tcaatggtcc cgacaacctg accttttttc agcggtgcgg taagctgcgg
    18901 ttccgttaac gtataactcg ctttcaggtt tttcagctgc ccacgcggta tggtcactga
    18961 gcccgcttca cctgccccga gattcacttc gctcttatca ccaaaccaga cgcgctgagt
    19021 cacaaaggtg gcatcaggtt taattggcgt cacggtttca aagaagcgga aaccccaggt
    19081 caataatttc tcagactcat taaaacggat acggtcggtt ttcgccccca acactacgga
    19141 gattaaacgc atatcgccct gggtagccga agcaaccaga ttatatcccg cgcctgccgt
    19201 ggttcctgtc ttcatgccat caacattcag attgctgctc cacagcagac ggttacggtt
    19261 aggctgacga attttgttga aggtgaactc tttctcttta tgaatggcgt actcttccgg
    19321 cacatcgtgg atcaatgctt tacccagcaa tgccatatcg cgcgcggtgc tgaactgccc
    19381 cggcgcatcc aggccgtgca ccgtctggaa ggtagtgttg gtcagaccca gttttttggc
    19441 ataaccattc atcagaccaa taaatgactc ctggctcccg gcaacgtaat cagccagcgc
    19501 aatacaggcg tcattaccgg actggataat cacacctttg ttcaagtctg ccaccgaaac
    19561 ctgatcgccc ggtttgagga acattaccga tgaaccacgc agtgccggat ttcccgtcgc
    19621 ccaggcatct ttaccgaccg tcaccatatc ggtgagttta atcttatcgg ccttaagcgc
    19681 ctgcccaacc acatagctgg tcatgatttt agtcaggctc gcgggatcca gtttctcatc
    19741 cgcattgcct tctgccagca ctttaccgct ggcgtaatcc attaaaatcc atgcacgcgc
    19801 atccacgctc ggcgcttcaa cggtttgttc cgccgcgaat gccgttgggg caaaaaggaa
    19861 taaaaatgca gaacccgctg caagaccacg aaggagagag gagtattgcg tcataaatgc
    19921 cacccgagta tccattcaaa aaaaatacgt tatatcgccg tatgataagt ttggtgatta
    19981 ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa gtttatgcaa
    20041 tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct taaaagttaa
    20101 ctttacttca ccatcggagt cctgttcagc ctgtggctgc ctgtcggaaa aggggtgagt
    20161 atgattacgc tgtggggtcg gaataattca actaacgtaa aaaaagtatt gctgacgctc
    20221 gaagaactgg aactacctta tgagcaaatt ctcgcgggcc gtgagtttgg gataaatcac
    20281 gatgctgatt ttctggcgat gaaccctaac gggctggtgc cgttgttgcg tgacgacgaa
    20341 agtgatctca ttctttggga atcaaacgcc attgtccgct atctggcggc acagtacggg
    20401 caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg aagcggaaaa atggatggac
    20461 tgggcaaacc agacgctcag taatgctcat cgcgggatcc tgatgggatt agtcagaaca
    20521 ccaccggaag agcgcgatca ggccgccatt gatgccagtt gcaaagagtg cgacgccctg
    20581 tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt tctctggcga cgagtttggt
    20641 gtgggcgata tcgctatcgc accgttcatc tacaatttgt ttaacgttgg cctgacctgg
    20701 acaccgcgtc caaatctgca acgctggtat cagcaactca ctgaacgccc cgcagtgcgc
    20761 aaagtggtga tgattcccgt tagctaattg cgtgggctaa ctttaagtaa ttccccactg
    20821 gactcgtcgg tgagaacgta taaataaccg tcgggtccag tgcgaacatc acgaattcgc
    20881 tgccctctgt ccgttaaaat acggccatct tctgtcactt tgtcgccgtt gacgctcatc
    20941 acaatgacat ctttatcttt cagcgcgcca ataaataatt tttgctgcca ctgggggaat
    21001 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
    21061 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
    21121 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
    21181 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
    21241 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
    21301 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
    21361 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
    21421 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
    21481 ggcatctggc gaaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
    21541 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
    21601 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
    21661 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
    21721 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
    21781 agtgcccagg gatggtcggg tttgtcttgc agtacttcga catttaccgt tgcaggagcc
    21841 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
    21901 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
    21961 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
    22021 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
    22081 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
    22141 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
    22201 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
    22261 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
    22321 cgtatgcggc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
    22381 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
    22441 tattttttga gcaaaatgct gtccaggatc aattattcac gccgaattaa caaaatttac
    22501 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
    22561 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
    22621 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt tgattcagag
    22681 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
    22741 gacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
    22801 gccattggtg aagcgttgaa tgaaaacggc aaggttattg tgaccggttg tctgggggca
    22861 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
    22921 tatgagcagg ttctggagca cgttcatcac tacgtgccga aaccgaaaca caacccattc
    22981 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
    23041 atttctgaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
    23101 ctggtgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
    23161 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
    23221 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
    23281 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
    23341 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
    23401 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
    23461 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
    23521 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
    23581 gcgcgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
    23641 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
    23701 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
    23761 attatcgacg aagtggacga agaaggcgca attggtcgca gcatggcaga tgcaccggaa
    23821 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
    23881 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
    23941 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
    24001 tgccgttttt cttactctta tacagccgct catccaccag cattttcaag cgttcgacgg
    24061 taggacattc atttgaatgt gctacaccga tactggtact taaattaatg cttcccacct
    24121 ggaaatgaac gatttctttt agaatcgata gcaactcttc cagcgttcca cccgtataaa
    24181 tcaccgcaaa ttcgtcaccg ccgaagcggt agactttacc tttatctacc gggatctgtg
    24241 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
    24301 cgttaatgtt tttgaattta tcgacgtcaa acaggcacaa ataaccgcct ttttccggca
    24361 gcgcttttaa atcttcgtca aaggattttc tgcccattgc tttggtcagg tcgtccagga
    24421 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
    24481 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
    24541 taatcactct gcgtaccaga tagaggcaac tggcacccga tatcactgcc accaatgcgg
    24601 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacat
    24661 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
    24721 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
    24781 ggttctcgct gtccatcgcc agcgattttg acagtaaaga aaaacgctcc agaccaagca
    24841 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
    24901 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
    24961 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
    25021 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
    25081 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
    25141 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
    25201 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
    25261 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
    25321 tttactaatt ttccactttt atcccaggcg gagaatggca tgggtttctg gtacagatac
    25381 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
    25441 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
    25501 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
    25561 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
    25621 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
    25681 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
    25741 atggtggtaa aacactcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
    25801 tgcagtaaga aatcatcctt cagcgacgca cgctgactaa aatgtctgaa ttcacccatt
    25861 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
    25921 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
    25981 gcgcccagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
    26041 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
    26101 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
    26161 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
    26221 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
    26281 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
    26341 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
    26401 ctgacgattt tattgcgttc atcaacacca aacaccgctt ccacggcttc cagaccacac
    26461 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
    26521 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
    26581 atttcggcat ctttcatttc cgcgatcgaa tttttaatgc tgataatctc tttcgcatcg
    26641 ccttcgcgaa ttttcagagc ctcgatttga tccgttgagt cgggcaaaaa tgccagcgta
    26701 ttaaccagct ctgtcatttc agaaaattgc cgacggaaca cacgagtggt cattaaataa
    26761 accaccagca atacgataat aatgaacgca aacggcagat aaaaaagttt atctgaggcg
    26821 ttcaataaat taccgccagg ataataaaaa tagatttcga ataatgcgtt gtttggcacc
    26881 gctgatgttt tttcgatata
//
LOCUS       DAZFZC010000073        26536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFZC010000073 DAZFZC010000000
VERSION     DAZFZC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.073"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            48..1517
                     /gene="gspA"
                     /locus_tag="ACHKXP_003351"
     CDS             48..1517
                     /gene="gspA"
                     /locus_tag="ACHKXP_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO7648295.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1519..1938
                     /gene="gspB"
                     /locus_tag="ACHKXP_003352"
     CDS             1519..1938
                     /gene="gspB"
                     /locus_tag="ACHKXP_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO7648296.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESEEKAGLRERVKNALNELER"
     gene            2176..2487
                     /gene="rpsJ"
                     /locus_tag="ACHKXP_003353"
     CDS             2176..2487
                     /gene="rpsJ"
                     /locus_tag="ACHKXP_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO7648297.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2520..3149
                     /gene="rplC"
                     /locus_tag="ACHKXP_003354"
     CDS             2520..3149
                     /gene="rplC"
                     /locus_tag="ACHKXP_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO7648298.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3160..3765
                     /gene="rplD"
                     /locus_tag="ACHKXP_003355"
     CDS             3160..3765
                     /gene="rplD"
                     /locus_tag="ACHKXP_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO7648299.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3762..4064
                     /gene="rplW"
                     /locus_tag="ACHKXP_003356"
     CDS             3762..4064
                     /gene="rplW"
                     /locus_tag="ACHKXP_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO7648300.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4082..4903
                     /gene="rplB"
                     /locus_tag="ACHKXP_003357"
     CDS             4082..4903
                     /gene="rplB"
                     /locus_tag="ACHKXP_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO7648301.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4920..5198
                     /gene="rpsS"
                     /locus_tag="ACHKXP_003358"
     CDS             4920..5198
                     /gene="rpsS"
                     /locus_tag="ACHKXP_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO7648302.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5213..5545
                     /gene="rplV"
                     /locus_tag="ACHKXP_003359"
     CDS             5213..5545
                     /gene="rplV"
                     /locus_tag="ACHKXP_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO7648303.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5563..6264
                     /gene="rpsC"
                     /locus_tag="ACHKXP_003360"
     CDS             5563..6264
                     /gene="rpsC"
                     /locus_tag="ACHKXP_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO7648304.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6277..6687
                     /gene="rplP"
                     /locus_tag="ACHKXP_003361"
     CDS             6277..6687
                     /gene="rplP"
                     /locus_tag="ACHKXP_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO7648305.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6687..6878
                     /gene="rpmC"
                     /locus_tag="ACHKXP_003362"
     CDS             6687..6878
                     /gene="rpmC"
                     /locus_tag="ACHKXP_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO7648306.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6878..7132
                     /gene="rpsQ"
                     /locus_tag="ACHKXP_003363"
     CDS             6878..7132
                     /gene="rpsQ"
                     /locus_tag="ACHKXP_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO7648307.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7297..7668
                     /gene="rplN"
                     /locus_tag="ACHKXP_003364"
     CDS             7297..7668
                     /gene="rplN"
                     /locus_tag="ACHKXP_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO7648308.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7679..7993
                     /gene="rplX"
                     /locus_tag="ACHKXP_003365"
     CDS             7679..7993
                     /gene="rplX"
                     /locus_tag="ACHKXP_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO7648309.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8008..8547
                     /gene="rplE"
                     /locus_tag="ACHKXP_003366"
     CDS             8008..8547
                     /gene="rplE"
                     /locus_tag="ACHKXP_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO7648310.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8562..8867
                     /gene="rpsN"
                     /locus_tag="ACHKXP_003367"
     CDS             8562..8867
                     /gene="rpsN"
                     /locus_tag="ACHKXP_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO7648311.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8901..9293
                     /gene="rpsH"
                     /locus_tag="ACHKXP_003368"
     CDS             8901..9293
                     /gene="rpsH"
                     /locus_tag="ACHKXP_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO7648312.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9306..9839
                     /gene="rplF"
                     /locus_tag="ACHKXP_003369"
     CDS             9306..9839
                     /gene="rplF"
                     /locus_tag="ACHKXP_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO7648313.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9849..10202
                     /gene="rplR"
                     /locus_tag="ACHKXP_003370"
     CDS             9849..10202
                     /gene="rplR"
                     /locus_tag="ACHKXP_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO7648314.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10217..10720
                     /gene="rpsE"
                     /locus_tag="ACHKXP_003371"
     CDS             10217..10720
                     /gene="rpsE"
                     /locus_tag="ACHKXP_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO7648315.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10724..10903
                     /gene="rpmD"
                     /locus_tag="ACHKXP_003372"
     CDS             10724..10903
                     /gene="rpmD"
                     /locus_tag="ACHKXP_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO7648316.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10907..11341
                     /gene="rplO"
                     /locus_tag="ACHKXP_003373"
     CDS             10907..11341
                     /gene="rplO"
                     /locus_tag="ACHKXP_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO7648317.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11349..12680
                     /gene="secY"
                     /locus_tag="ACHKXP_003374"
     CDS             11349..12680
                     /gene="secY"
                     /locus_tag="ACHKXP_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO7648318.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12712..12828
                     /gene="rpmJ"
                     /locus_tag="ACHKXP_003375"
     CDS             12712..12828
                     /gene="rpmJ"
                     /locus_tag="ACHKXP_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO7648319.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12975..13331
                     /gene="rpsM"
                     /locus_tag="ACHKXP_003376"
     CDS             12975..13331
                     /gene="rpsM"
                     /locus_tag="ACHKXP_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO7648320.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13348..13737
                     /gene="rpsK"
                     /locus_tag="ACHKXP_003377"
     CDS             13348..13737
                     /gene="rpsK"
                     /locus_tag="ACHKXP_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO7648321.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13771..14391
                     /gene="rpsD"
                     /locus_tag="ACHKXP_003378"
     CDS             13771..14391
                     /gene="rpsD"
                     /locus_tag="ACHKXP_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO7648322.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14417..15406
                     /gene="rpoA"
                     /locus_tag="ACHKXP_003379"
     CDS             14417..15406
                     /gene="rpoA"
                     /locus_tag="ACHKXP_003379"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO7648323.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15447..15830
                     /gene="rplQ"
                     /locus_tag="ACHKXP_003380"
     CDS             15447..15830
                     /gene="rplQ"
                     /locus_tag="ACHKXP_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO7648324.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15937..16305
                     /locus_tag="ACHKXP_003381"
     CDS             15937..16305
                     /locus_tag="ACHKXP_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO7648325.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16316..16741
                     /gene="zntR"
                     /locus_tag="ACHKXP_003382"
     CDS             16316..16741
                     /gene="zntR"
                     /locus_tag="ACHKXP_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO7648326.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16797..17015
                     /gene="arfA"
                     /locus_tag="ACHKXP_003383"
     CDS             16797..17015
                     /gene="arfA"
                     /locus_tag="ACHKXP_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO7648327.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(17012..17422)
                     /gene="mscL"
                     /locus_tag="ACHKXP_003384"
     CDS             complement(17012..17422)
                     /gene="mscL"
                     /locus_tag="ACHKXP_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO7648328.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17552..18928)
                     /gene="trkA"
                     /locus_tag="ACHKXP_003385"
     CDS             complement(17552..18928)
                     /gene="trkA"
                     /locus_tag="ACHKXP_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO7648329.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18950..20239)
                     /gene="rsmB"
                     /locus_tag="ACHKXP_003386"
     CDS             complement(18950..20239)
                     /gene="rsmB"
                     /locus_tag="ACHKXP_003386"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO7648330.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKTGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20285..21232)
                     /gene="fmt"
                     /locus_tag="ACHKXP_003387"
     CDS             complement(20285..21232)
                     /gene="fmt"
                     /locus_tag="ACHKXP_003387"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO7648331.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVAELQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21247..21756)
                     /gene="def"
                     /locus_tag="ACHKXP_003388"
     CDS             complement(21247..21756)
                     /gene="def"
                     /locus_tag="ACHKXP_003388"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO7648332.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21886..23010
                     /gene="dprA"
                     /locus_tag="ACHKXP_003389"
     CDS             21886..23010
                     /gene="dprA"
                     /locus_tag="ACHKXP_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO7648333.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HAQHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     CVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGSHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22982..23455
                     /gene="smg"
                     /locus_tag="ACHKXP_003390"
     CDS             22982..23455
                     /gene="smg"
                     /locus_tag="ACHKXP_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO7648334.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23484..24026
                     /locus_tag="ACHKXP_003391"
     CDS             23484..24026
                     /locus_tag="ACHKXP_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO7648335.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCINYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24031..24603
                     /gene="tsaC"
                     /locus_tag="ACHKXP_003392"
     CDS             24031..24603
                     /gene="tsaC"
                     /locus_tag="ACHKXP_003392"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO7648336.1"
                     /translation="MNNNLQRDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24608..25426
                     /gene="aroE"
                     /locus_tag="ACHKXP_003393"
     CDS             24608..25426
                     /gene="aroE"
                     /locus_tag="ACHKXP_003393"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO7648337.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANMTVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25423..25680
                     /locus_tag="ACHKXP_003394"
     CDS             25423..25680
                     /locus_tag="ACHKXP_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO7648338.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25656..26210)
                     /locus_tag="ACHKXP_003395"
     CDS             complement(25656..26210)
                     /locus_tag="ACHKXP_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO7648339.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6780 a         6260 c         6939 g         6557 t
ORIGIN      
        1 attattttta tgattaagga taatcaataa ttaaaacagg aagttctatg tctacgagaa
       61 gagaagttat tctttcctgg ttgtgtgaga aacgtcaaac ctggcgtcta tgctatttgt
      121 tgggtgaggc tggaagtgga aaaacctggc tggcgcagca actgcaaaaa gataaacatc
      181 gccgtgtgat tactttaagc ctcgttgttt cctggcaagg taaggccgca tggatcgtta
      241 ccgacgataa cgcggctgaa cagggctgcc gtgacagcgc ctggacgcga gatgagatgg
      301 cggggcaatt actgcatgcg cttcatcgaa ctgatagtcg ctgccctctt attattattg
      361 aaaatgctca cctgaatcac cgccgcatac ttgatgatct ccaacgcgca atatctctta
      421 ttcctgacgg tcaatttttg ctgatcggca gacccgatcg aaaagtcgaa cgtgatttta
      481 aaaaacaggg cattgaactt gtctcgatag gacgtctgac ggagcacgaa cttaaggcaa
      541 gcatccttga aggtcagaat attgaccaac ccgatctcct tttaaccgcc agagttctga
      601 aacggatagc tttattatgt cggggcgatc gcagaaagct ggcgcttgcc ggcgaaacaa
      661 ttcgcttatt acagcaagcc gagcagacca gcgttttcac agcaaaacaa tggcgaatga
      721 tttaccgcat attgggtgat aaccgccccc ggaaaatgca gcttgccgtg gtgatgagtg
      781 gaaccattat cgctcttacc tgtggttggc tactgctgtc ctcttttaca gctacgttac
      841 ccgttcctgc gtggctgata cccgtaacgc cagtagttaa acaagatatg actaaggata
      901 ttgctcatgt ggtgatgcgc gatagtgagg cgctaagcgt gttatacggc gtatgggggt
      961 acgaggtccc agctgattcg gcctggtgcg atcaggcagt aagagcgggg ctggcatgta
     1021 aaagtgggaa tgcttcttta caaacattgg tagatcagaa tttgccctgg atcgcatcgc
     1081 ttaaagtcgg ggacaaaaaa ttgcctgttg tggtcgttcg cgtcggtgag gctagcgttg
     1141 atgtcctcgt aggtcagcaa acgtggacgc tgacacataa atggtttgag tcggtatgga
     1201 cgggtgatta tcttctgttg tggaaaatgt cgccggaagg ggagagtacc attacgcgtg
     1261 atagcagcga ggaagagatt ctctggctgg aaacgatgtt aaaccgcgca ttgcatattt
     1321 cgacagaacc ttcggcggag tggcgtccct tgttggtaga aaaaataaag caatttcaaa
     1381 aaagccacca cttgaaaact gacggtgttg ttggcttttc aacgttagta catctctggc
     1441 aggtggcagg ggagagtgcc tacttatatc gggatgaagc aaatatttcc ccggaaacaa
     1501 cagtgaaggg gaaatgacat gtttgaattc tatatcgccg cgcgtgagca gaaagaaaca
     1561 ggtcaccctg gtatcttctc ccgacaaaaa cacagcacca taatatatgt aatttgttta
     1621 ttactgattt gcctgtggtt tgcaggaatg gtgctggttg gtgggtatgc caggcagcta
     1681 tgggtgcttt ggatagtaaa agccgaagtc actgtagagg ctgaaacgcc cgcttttaaa
     1741 caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga atccgtcgag
     1801 gaggaagatg acccaggcgt cgcggttgag aatgccccct cttcttctga agacgaagaa
     1861 aatactgtgg aagaaagcga agagaaagcc ggtttaagag agcgtgtcaa aaatgcactg
     1921 aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc ttgcgttcgg
     1981 tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca atctgaacct
     2041 cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg taagaacggt
     2101 tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt ataaaataat
     2161 tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc gtttgatcat
     2221 cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac tggtgcgcag
     2281 gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct gatctccccg
     2341 cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg tctggttgac
     2401 atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct ggctgccggt
     2461 gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag gttgaaacaa
     2521 tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa gacggcgttt
     2581 ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt aaagacctgg
     2641 ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct aaccgtgtga
     2701 ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt ggtctgtggg
     2761 aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc gttgaactgt
     2821 ttgctgacgt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt ttcgcaggta
     2881 ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc ttgtctcacc
     2941 gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa ggcaagaaaa
     3001 tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta gtacgcgttg
     3061 acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc ggtagcgacc
     3121 tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt attgaaagac
     3181 gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa cgaagcgctg
     3241 gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg tgctcagaag
     3301 actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg caccggccgt
     3361 gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac ctttgctgct
     3421 cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc gctgaaaagc
     3481 atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt ctctgtagaa
     3541 gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga agatgtgctg
     3601 atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct gcacaaggtt
     3661 gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga caaagtcgta
     3721 atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt gaagaacgtc
     3781 tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg atggaaaaat
     3841 ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc aaagctgctg
     3901 tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt aaagggaaag
     3961 ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct tacgtcaccc
     4021 tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga ggagtaatac
     4081 aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag ttaaagtggt
     4141 taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa acagcaaatc
     4201 cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg gccacaagca
     4261 ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag ttgttgaacg
     4321 tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca aagacggtga
     4381 acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc agtctggcgt
     4441 tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg ttggttctac
     4501 tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt ccgctggtac
     4561 ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc gttctggtga
     4621 aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca atgctgagca
     4681 tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc gtccgaccgt
     4741 tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag gtcgtaactt
     4801 tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga cccgcagcaa
     4861 caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga ggataagcca
     4921 tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag aaggtagaga
     4981 aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt tcaacgatct
     5041 ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac gttccggtat
     5101 ttgtaaccga cgaaatggtt ggtcacaaac tgggtgaatt cgcaccgact cgtacttatc
     5161 gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag agatggaaac
     5221 tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg ttgctgacct
     5281 gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca acaagaaagc
     5341 ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac acaacgatgg
     5401 cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc cgagcatgaa
     5461 gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca ccagccacat
     5521 cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca gaaagtacat
     5581 cctaatggta ttcgcctggg tattgtaaaa ccatggaact ctacctggtt tgcgaacacc
     5641 aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct gactaaggaa
     5701 ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag catccgtgta
     5761 accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga cgtagaaaaa
     5821 ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat cgccgaagtt
     5881 cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca gctggaacgt
     5941 cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg tctgggcgct
     6001 aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc acgtaccgaa
     6061 tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga ctacaacacc
     6121 tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt caaaggcgag
     6181 atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca gcctaaaaag
     6241 cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa gcgtacaaaa
     6301 ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga tgttagcttc
     6361 ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca gatcgaagca
     6421 gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat ccgtgtgttc
     6481 ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg taaaggtaac
     6541 gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat ggacggtgtt
     6601 ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc gattaaaacc
     6661 acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag aagagcgttg
     6721 aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg cgtatgcagg
     6781 ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc gatgtcgcac
     6841 gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa tccgtactct
     6901 gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta tcgaacgttt
     6961 tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc acgtacatga
     7021 cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc gtccgctgtc
     7081 caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt aatacagtac
     7141 actctctcaa tacgaataaa cggctcagaa atgagccgtt tattttttct acccatatcc
     7201 ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac ctgattttcg
     7261 ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca gactatgctg
     7321 aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct gggtggctcg
     7381 caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga agcaattccg
     7441 cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac caagaagggt
     7501 gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt tcttctgaac
     7561 aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg tgagcttcgt
     7621 agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg agcgaatcat
     7681 ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag ataaaggtaa
     7741 acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag gtatcaacct
     7801 ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca tcgttgaaaa
     7861 agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg gcaaggctga
     7921 ccgtgtaggc tttagattcg aagacggtaa aaaagtccgt ttcttcaagt ctaacagcga
     7981 aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta caaagacgaa
     8041 gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt ccctcgggtc
     8101 gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa actgctggat
     8161 aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac caaagcacgc
     8221 aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa agtaactctg
     8281 cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt acctcgtatc
     8341 cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta cagcatgggt
     8401 gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg cgttcgtggt
     8461 ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc tctgctggct
     8521 gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag caatcaatga
     8581 aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa cgcgctgaac
     8641 tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac gctgttctca
     8701 agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc tgccgtcaaa
     8761 caggtcgtcc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag gtccgtgaag
     8821 ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg tcaccaattg
     8881 aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg ctgacccgta
     8941 tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc aagctgaaag
     9001 tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa gttgaaggcg
     9061 acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct gttgtagaaa
     9121 gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat gagctgccga
     9181 aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt atgactgatc
     9241 gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc taatcggagg
     9301 aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt gacgtaaaaa
     9361 tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt actctcaacg
     9421 atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt gatggttacg
     9481 cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg gttatcggtg
     9541 ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt gcagcggtta
     9601 aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat cagctgcctg
     9661 cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc gctgataagc
     9721 aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag ccttataaag
     9781 gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag aagaagtaag
     9841 gtaacactat ggataagaaa tctgctcgta tccgtcgtgc gacccgcgca cgccgcaagc
     9901 tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac atttacgcac
     9961 aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta gaaaaagcta
    10021 tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg ggtaaagctg
    10081 tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt tccgggttcc
    10141 aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc cttcagttct
    10201 aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc aggaaaagct
    10261 gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct ccttcacagc
    10321 tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta aagcgcgtga
    10381 agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga ttaacgtcgc
    10441 gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt ctcgcgtatt
    10501 catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc gcgccgttct
    10561 ggaagtcgct ggggttcata acgttctggc taaagcctat ggttccacca acccgatcaa
    10621 cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa tggtcgctgc
    10681 caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa agactattaa
    10741 aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa cgctgcttgg
    10801 cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg ctattcgcgg
    10861 tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc gtttaaatac
    10921 tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg gtatcggttc
    10981 tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt ctggcggtgg
    11041 cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc cgaaattcgg
    11101 cttcacttct cgtaaagcag cgattacagc cgaaattcgt ctgtctgacc tggctaaagt
    11161 agaaggcggt gtagtagacc tgaacacgct gaaagcggct aacattatcg gtatccagat
    11221 cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg ttcgtggcct
    11281 gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa tcgaggaata
    11341 agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt ggcttaggcg
    11401 agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt attggctctt
    11461 ttattccgat ccctggtatt gatgccgctg tacttgccaa actgcttgag caacagcgag
    11521 gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt gcttctatct
    11581 ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg ctgacggtgg
    11641 ttcacccaac gttggcagaa attaagaaag aaggggagtc tggtcgtcgt aagatcagcc
    11701 agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt attgctaccg
    11761 gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt gcattctact
    11821 tcaccgctgt tgtaagtctg gtcacaggaa ccatgttcct gatgtggttg ggcgaacaga
    11881 ttactgaacg aggtatcggc aacggtattt caatcattat cttcgccggt attgtcgcgg
    11941 gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac ctgcacttcc
    12001 tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt gtatttgttg
    12061 agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt cgtcgtgtct
    12121 atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta atcccggcaa
    12181 tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc gggggcggta
    12241 ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa ccgctttatg
    12301 tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg ttggttttca
    12361 acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca ggaattcgtc
    12421 cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc ctggttggtg
    12481 cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca atgaaagtac
    12541 cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg gactttatgg
    12601 ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag aaggcgaacc
    12661 tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa aatgaaagtt
    12721 cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga tggtgtcatc
    12781 cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt ttttcgcata
    12841 tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt tgtatgtctg
    12901 tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta catattaaat
    12961 agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat aagcatgccg
    13021 taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc atcctggctg
    13081 cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa atcgacacgc
    13141 tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa atcagcatga
    13201 gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt cgtggtctcc
    13261 cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg cgcaaaccga
    13321 tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc acgtaaacgt
    13381 gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa caacaccatc
    13441 gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg tggttccggt
    13501 ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga gcgttgcgct
    13561 gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg tccgggtcca
    13621 ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac taacattact
    13681 gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg cgtataacgc
    13741 ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc taagctcaag
    13801 ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc gatcgatacc
    13861 aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg tctgtctgac
    13921 tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt gctggagcgt
    13981 cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg tgaaaacctg
    14041 ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt cggtgccact
    14101 cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg tcgtgttgtt
    14161 aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga gaaagcgaag
    14221 aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa gccaacctgg
    14281 ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga gcgttctgat
    14341 ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta aagcttagta
    14401 ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc gcctggttga
    14461 tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag agcgtggctt
    14521 tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc cgggttgcgc
    14581 ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag aaggcgttca
    14641 ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag ttcagggcaa
    14701 agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg cagccgatat
    14761 cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc acctgaccga
    14821 tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt atgtgccggc
    14881 ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc tggtcgacgc
    14941 atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg tagaacagcg
    15001 taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg atcctgaaga
    15061 ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg ttgacttacg
    15121 tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga tcctgctgcg
    15181 ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag cagaagctat
    15241 ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa cgcctaacct
    15301 tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac tgtctctggg
    15361 catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg atcacaggtt
    15421 aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt caactgaacc
    15481 gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg gttcgtcatg
    15541 aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt gagccgctga
    15601 ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc cgtactcgtg
    15661 ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg agccgtgccg
    15721 gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg ccgatggctt
    15781 acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa tctgaagcaa
    15841 cgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac ttatctacaa
    15901 tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga ccagtgggca
    15961 gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc aggtagcggc
    16021 gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc ggggtatcgc
    16081 ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga agcaattcag
    16141 cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga ggttagccgt
    16201 cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga ttttttacgc
    16261 ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag tatgtatgta
    16321 tcgcattggt gagctggcaa aaatggcgga agtaacaccc gacacgattc gttattacga
    16381 aaaacagcag atgatggagc atgaagtgcg tactgaaggt gggtttcgcc tatataccga
    16441 aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt tcagtctgga
    16501 gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct gtcaggagtc
    16561 aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg agttgcagag
    16621 tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggactgctc atagcagtgt
    16681 ttattgttcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga gtggttgttg
    16741 attttttgca ctggcaggat tacactcgcg ccgttaaata accaactgga gtttttatga
    16801 gtcgatatca gcatactaaa gggcagataa aggataatgc gatagaagca ttactacatg
    16861 atcccttatt ccgacagcgc gtagagaaaa ataagaaggg gaaaggcagt tacatgcgaa
    16921 aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta aatcactttt
    16981 ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc tctttcagca
    17041 aatcacgaat ttctgtcagt aatacttctt ctttagttgg tgcaggtgcg gctgctggtt
    17101 cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg gcaaaggcca
    17161 caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca acagcaggga
    17221 tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaaatcgatc ccgccaatta
    17281 ataagcccag aggaggcatg atgatatcgg caaccagtga agagacaatc ttcccgaatg
    17341 ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc gcaaattcgc
    17401 gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca taagtctaac
    17461 aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata atcaaaggaa
    17521 aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt ggctggaaga
    17581 gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg tgatcgcctt
    17641 gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc accgctccaa
    17701 taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg cgtgaggtgc
    17761 tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc aatgaggaaa
    17821 caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt gcttgttgtg
    17881 gtgaaatcgc aatatcgata acgctcccct gaaccagatc cacataagcg cgacgctgga
    17941 tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac atgatattgg
    18001 cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt tcttcggcca
    18061 gtagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgtaac ttttccgcca
    18121 gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct ttttccagac
    18181 gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc ttatacggtt
    18241 tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg gcaataaaga
    18301 acacttcatc accagcttca acaatggtcg aaccttgcgg acgaatgggg cgatcgtggc
    18361 ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg
    18421 cattaccaat cagcgggccg ccataatagg ctttaaccac agccaggctg actttaccct
    18481 cagcgaagtt caccacctgc aatgcgccgg gatactcaat cagtcggtaa atattatcga
    18541 taaccaactg ctctggtgcg atcagatgat caatcggcac agcatctgaa tgaaatagct
    18601 tatcggcatc gcgcacgtag tctggtgagc ggatacgagc gatgcgatta ggggtgttga
    18661 aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg gttacagcaa
    18721 ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga gagccatgcc
    18781 cctgcacgac ccgcaggtca aatttatcct gtaaggtccg cagacgctca ccgttggtat
    18841 cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct
    18901 ggccggcacc cagaatgata attttcatca gtcgcgaccc gttatctcat cactttttga
    18961 ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggtagattt tgtttacccg
    19021 gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc aaaaaggctt
    19081 taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc agagttccac
    19141 cggtttttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc gcgagttccg
    19201 ggatatcgcg atcgcgacgt aaccatttaa tatctggatg gcgacgaatc acaccggttg
    19261 ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac cattgagaag
    19321 ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt aaattgtcgt
    19381 aaacccgaga gaggcgctgt tcgtcgatat caaccgcaac aacctgcgct tctggtgcca
    19441 cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaaa atgtgttcac
    19501 cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga acggtaaccc
    19561 atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccaga cgtacagcat
    19621 cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccagcaat gcaagccagc
    19681 tgtcgcggga atgatgcgta cgattaatac gcagccacat tggcggacgc tggttattgg
    19741 cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc agcaaccagg
    19801 aaggatgcag ataacgtgca tcactggcat taaactcggc taataactct tcttgctgac
    19861 gctggaactg gcgtaataca ccgtttatca accctttaag ttgcggacgc ttaattgcga
    19921 tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta taaagcagtt
    19981 gatacaaacc aaccataatc aggtaatgca cggtccgctg tttgccggtc atcggacggg
    20041 ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg ccaaagcaca
    20101 actcttgaag aagtgctttg tctttatcgg aaactttttg ctggagcggt ggcagaatgt
    20161 tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc atgctacgta
    20221 aattacgttg ttttttcata agtataaaaa cgcccggcaa gaccgggctt agaagagtgg
    20281 actatcagac cagacggttg cccggaacaa accattcccg acgagagttc aggaggtctt
    20341 gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg atgccatcac
    20401 cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt gcagcgttgg
    20461 ttgccgtatc aatgaccgat gctttccaga ctttaaccgg ctgtccttca atttccagcc
    20521 agctcattgg ccatggattg aaagcgcgaa tgcagcgttc aagctgtgct gccgaaagtg
    20581 accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca agagtttcgt
    20641 cctgaacttc tggtttcgcc gtgccgtctg ccagttgttt caacgtggtg ataagccctt
    20701 gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct gcagtaatcg
    20761 ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt tgcataatgg
    20821 tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca gcaccgcgcc
    20881 agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc tccagcactg
    20941 ctttcggcag aattaaacca taggcgacga cgaccataac atcagcctgc agttcggcga
    21001 ccagttgctg gttttcttgt ggacgcaggg aaacaggttg aaaaacgggc agaccttttt
    21061 cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct gccggtcggt
    21121 ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg tcgagatgac
    21181 gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac acgttagttc
    21241 ttatccttaa gcccgggctt tcagacgatc cagtttttca actttctgac gaatacgttg
    21301 ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat ccatctcatg
    21361 ctgaatacag atggctaaca gaccgtctgc ttccagttca aatggtttac cgtcgcggtc
    21421 aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt cagggatcga
    21481 caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctctg gattgattaa
    21541 cactagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt gatggatatc
    21601 aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga acatatcatc
    21661 gacgatacgc tgaatttctg cattcacttc ttctaccggt ttagcaactt tgcgaagccg
    21721 ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat gtgttcagga
    21781 gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca gcatcactga
    21841 ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt cgatacagaa
    21901 atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt ccgtatcgct
    21961 cactgggtgg caaaacagtc gcatattgat gcggttgtat tgcagcaaac agggcttaca
    22021 ttgcggcagg cacaacgctt tctttcattt ccacgaaaga gtatcgaaag ctcactttgt
    22081 tggttggagc aacccaacca tcatttaatt cctgcggaca gcgaatttta tcctcctcaa
    22141 cttctggcga cgacagatta ccccggcgca ctgtttgttg aaggagaact gcacgcgcag
    22201 cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg cgagcgatgg
    22261 ggacgattat tttgcgaaac tctggcgacg cgtggagtga caattacgag tggactggcg
    22321 cgtggaatcg atggtgtagc gcataaagca gccttacagg taaattgcgt cagcattgct
    22381 gtattgggga atggacttaa taccattcat ccccgccgtc atgcccgact ggctgccagt
    22441 ctgcttgaac aggggggcgc tctcgtctcg gaatttcccc tcgatgttcc accccttgct
    22501 tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt actggtggtg
    22561 gaagcggctt tgcgtagtgg ttcgctggtg acagcacgtt gtgcgcttga gcaggggcga
    22621 gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggtc tcactggtta
    22681 ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt
    22741 ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga tcagcaagac
    22801 gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac acctgttgac
    22861 gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact actcgaactg
    22921 gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag gagggcatgc
    22981 catgttcgac gtactaatgt atttgtttga aacctatatt cacacagaag ctgagttgcg
    23041 tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc gagaagatat
    23101 ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt tggcagaacc
    23161 gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag agtgtgaacg
    23221 actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg tgctcaacct
    23281 tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag agttcgaact
    23341 ggatgatctg aaatgggtga tcctgatggt gttgttcaat attccgggct gcgaaaatgc
    23401 gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc attaatcatt
    23461 tctttaattc agcataagtt gttatggcga aatcagcact gttcacggtg cgtaataatg
    23521 agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac ggtccgtttc
    23581 ttggatgctc acagtatccg gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg
    23641 gacatatcgt caaagttctg gaggggcagg tttgccctgc atgtggcgca aatctggtat
    23701 tacgccaggg acgctttggt atgtttattg gttgcattaa ctaccctgaa tgcgaacata
    23761 ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt cggacgggcc
    23821 atctggtcca gcgccgctcc cgttatggca aaacatttca ctcttgtgat cgctacccgg
    23881 agtgtcaatt tgccattaac ttcaaaccca tagctggaga atgccctgag tgtcattatc
    23941 cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat
    24001 gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaaag agacgctatc
    24061 gcagctgcga tagatgttct caatgaagaa cgtgtcatcg cctatccaac ggaagccgtt
    24121 ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgcgactgtt ggagttaaaa
    24181 cagcgtccgg ttgataaggg gctgatttta atcgcagcaa attacgagca gcttaaaccc
    24241 tatattgatg acaccatgtt gactgacgtg cagcgtgaaa ccattttttc ccgctggcca
    24301 ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc
    24361 tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt gtgccaggct
    24421 tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc ttgtcgaaca
    24481 gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg
    24541 gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt tcgacagggg
    24601 taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca aatcgccatt
    24661 cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc gcgtgttggc
    24721 acccatcaat gatttcatca acacactgaa cgctttcttt agtgctggtg gtaaaggtgc
    24781 gaatatgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc ttactgaacg
    24841 ggcagcgttg gctggtgctg ttaataccct catgcggtta gaagatggac gcctgctggg
    24901 tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt ttatccgccc
    24961 tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac tactgccact
    25021 cctttccctg gactgtgcgg tgacaataac taatcggacg gtatcccgcg cggaagagtt
    25081 ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg agtatggacg aactggaagg
    25141 tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg atattccggc
    25201 gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct atcagaaagg
    25261 aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt
    25321 aggaatgctg gtggcacagg cggctcatgc ctttcttctc tggcacggtg ttctgcctga
    25381 cgtagaacca gttataaagc aattgcagga ggaattgtcc gcgtgaatca ggccatccag
    25441 tttccggaca gggaagagtg ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg
    25501 aatggtatgc aactgacatg cgcgatctct ggcgagagtc tggcgtatcg ctttactgga
    25561 gatacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct ggaagaagaa
    25621 gcggaaaact taattcagga acaaagtgaa gatgatcaag gctgggtctg gttaccctga
    25681 tccagatatt cgtccttcca tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc
    25741 tcttcatcac ttaacgggcg gatctgtttg acggggctac cgagatacag atatccgctc
    25801 tccagccgtt tattttgtgg gaccagacta cccgcaccaa tcatcacatc atcttctact
    25861 attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag
    25921 ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa tgggttgcca
    25981 tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg gatattggtg
    26041 cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg ccagatcccc
    26101 acatcatcag ccagacgaac gtcaccaatc acgacactgc tatcgtcgat cattacgcgc
    26161 tgaccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat acttacctca
    26221 gcaataaatg atttactaat gactttgggg gcattattgg ccttgtgcaa gtcttttagt
    26281 atgcaaaaaa gcaccgtttt gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca
    26341 gaaaaaaaga tcaaaaaaat acttgtgcaa aaaattggga tccctataat gcgcctccgt
    26401 tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga aaaaaatcct
    26461 gaaattcagg gttgactctg aaagaggaaa gcgtaatata cgccacctcg cgacagtgag
    26521 ctgaaagccg cgtcgc
//
LOCUS       DAZFZC010000074        26001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFZC010000074 DAZFZC010000000
VERSION     DAZFZC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.074"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..96
                     /locus_tag="ACHKXP_003396"
     CDS             <1..96
                     /locus_tag="ACHKXP_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO7648340.1"
                     /translation="GFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            242..1474
                     /gene="mdtM"
                     /locus_tag="ACHKXP_003397"
     CDS             242..1474
                     /gene="mdtM"
                     /locus_tag="ACHKXP_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO7648341.1"
                     /translation="MAGLKATHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIRLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAIMGFISFVGLLLAMPETVKRGAVSFSAKSVLRDFRNVFCNRQFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            1515..2795
                     /gene="yjiN"
                     /locus_tag="ACHKXP_003398"
     CDS             1515..2795
                     /gene="yjiN"
                     /locus_tag="ACHKXP_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO7648342.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            2911..4062
                     /gene="yjiM"
                     /locus_tag="ACHKXP_003399"
     CDS             2911..4062
                     /gene="yjiM"
                     /locus_tag="ACHKXP_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO7648343.1"
                     /translation="MSLVTDLPAIFDQFSEARQTGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            4072..4839
                     /gene="yjiL"
                     /locus_tag="ACHKXP_003400"
     CDS             4072..4839
                     /gene="yjiL"
                     /locus_tag="ACHKXP_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO7648344.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTNVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            4836..5093
                     /gene="ytiA"
                     /locus_tag="ACHKXP_003401"
     CDS             4836..5093
                     /gene="ytiA"
                     /locus_tag="ACHKXP_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO7648345.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            5158..6018
                     /gene="yjiK"
                     /locus_tag="ACHKXP_003402"
     CDS             5158..6018
                     /gene="yjiK"
                     /locus_tag="ACHKXP_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO7648346.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            6086..7264
                     /gene="yjiJ"
                     /locus_tag="ACHKXP_003403"
     CDS             6086..7264
                     /gene="yjiJ"
                     /locus_tag="ACHKXP_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648347.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(7277..7831)
                     /gene="kptA"
                     /locus_tag="ACHKXP_003404"
     CDS             complement(7277..7831)
                     /gene="kptA"
                     /locus_tag="ACHKXP_003404"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO7648348.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            8081..8764
                     /gene="yjiH"
                     /locus_tag="ACHKXP_003405"
     CDS             8081..8764
                     /gene="yjiH"
                     /locus_tag="ACHKXP_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO7648349.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            8761..9222
                     /gene="yjiG"
                     /locus_tag="ACHKXP_003406"
     CDS             8761..9222
                     /gene="yjiG"
                     /locus_tag="ACHKXP_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO7648350.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9235..10407
                     /gene="iadA"
                     /locus_tag="ACHKXP_003407"
     CDS             9235..10407
                     /gene="iadA"
                     /locus_tag="ACHKXP_003407"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7648351.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10472..11383
                     /gene="hypT"
                     /locus_tag="ACHKXP_003408"
                     /gene_synonym="qseD"
     CDS             10472..11383
                     /gene="hypT"
                     /locus_tag="ACHKXP_003408"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO7648352.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11376..11768)
                     /gene="iraD"
                     /locus_tag="ACHKXP_003409"
     CDS             complement(11376..11768)
                     /gene="iraD"
                     /locus_tag="ACHKXP_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO7648353.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(11765..11848)
                     /gene="idlP"
                     /locus_tag="ACHKXP_003410"
     CDS             complement(11765..11848)
                     /gene="idlP"
                     /locus_tag="ACHKXP_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO7648354.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(11964..12107)
                     /gene="ytiD"
                     /locus_tag="ACHKXP_003411"
     CDS             complement(11964..12107)
                     /gene="ytiD"
                     /locus_tag="ACHKXP_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO7648355.1"
                     /translation="MADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            12441..13271
                     /gene="yjiC"
                     /locus_tag="ACHKXP_003412"
     CDS             12441..13271
                     /gene="yjiC"
                     /locus_tag="ACHKXP_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO7648356.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(13412..14185)
                     /gene="uxuR"
                     /locus_tag="ACHKXP_003413"
     CDS             complement(13412..14185)
                     /gene="uxuR"
                     /locus_tag="ACHKXP_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO7648357.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSSDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(14400..15860)
                     /gene="uxuB"
                     /locus_tag="ACHKXP_003414"
     CDS             complement(14400..15860)
                     /gene="uxuB"
                     /locus_tag="ACHKXP_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO7648358.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(15941..17125)
                     /gene="uxuA"
                     /locus_tag="ACHKXP_003415"
     CDS             complement(15941..17125)
                     /gene="uxuA"
                     /locus_tag="ACHKXP_003415"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO7648359.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            17465..18808
                     /gene="gntP"
                     /locus_tag="ACHKXP_003416"
     CDS             17465..18808
                     /gene="gntP"
                     /locus_tag="ACHKXP_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO7648360.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(18981..19883)
                     /gene="fimH"
                     /locus_tag="ACHKXP_003417"
     CDS             complement(18981..19883)
                     /gene="fimH"
                     /locus_tag="ACHKXP_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO7648361.1"
                     /translation="MKRVINLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVVIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIAVTFVYQ"
     gene            complement(19903..20346)
                     /locus_tag="ACHKXP_003418"
     CDS             complement(19903..20346)
                     /locus_tag="ACHKXP_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7648362.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNSG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(20419..20949)
                     /gene="fimF"
                     /locus_tag="ACHKXP_003419"
     CDS             complement(20419..20949)
                     /gene="fimF"
                     /locus_tag="ACHKXP_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO7648363.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAVAGLGIQLLNEQQNQIPINAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(20959..23595)
                     /gene="fimD"
                     /locus_tag="ACHKXP_003420"
     CDS             complement(20959..23595)
                     /gene="fimD"
                     /locus_tag="ACHKXP_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO7648364.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMELNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLVGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(23662..24387)
                     /gene="fimC"
                     /locus_tag="ACHKXP_003421"
     CDS             complement(23662..24387)
                     /gene="fimC"
                     /locus_tag="ACHKXP_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO7648365.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(24424..24963)
                     /gene="fimI"
                     /locus_tag="ACHKXP_003422"
     CDS             complement(24424..24963)
                     /gene="fimI"
                     /locus_tag="ACHKXP_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7648366.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(25028..25582)
                     /gene="fimA"
                     /locus_tag="ACHKXP_003423"
     CDS             complement(25028..25582)
                     /gene="fimA"
                     /locus_tag="ACHKXP_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7648367.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPMTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGNELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         6180 a         6605 c         6442 g         6774 t
ORIGIN      
        1 ggattccata atgattccac gccccgttat gacaaatctg ccgccgatct tgcctggcaa
       61 aggacactga aatggtttga taaatatctc tcctgatagg tttatctctt acgggattac
      121 gtcttaaaca agcatgaaaa aatagcgtgc gcaaaagtcg ttctttgcct aaaacatcgc
      181 tatatatggc gatatatagc gaataaggtg aacgatgaat aaccattttg gtaaaggctt
      241 aatggcggga ttaaaagcaa cgcatgccgc cacgctgttt ttcccgatgg cgttgatttt
      301 gtatgatttt gccgcgtacc tgtcgacgga cctgattcag ccggggatca ttaatgtggt
      361 ccgtgatttt aatgccgatg tcagtctggc cccggcagcc gtcagtctct atctcgcggg
      421 cggtatggcg ttacagtggc tgctggggcc gctttccgac aggattggcc gcaggccggt
      481 gctgattacc ggggcgctaa tatttaccct tgcctgcgct gcgacaatgt tcacaacgtc
      541 tatgacacag tttcttatcg cgcgtgcaat tcagggcacc agtatctgtt ttattgccac
      601 cgttggttat gtcacggtgc aggaggcgtt cggccagaca aaagggatca ggttgatggc
      661 gattatcacc tccatcgtac tgattgcgcc gattatcggc ccgctttccg gcgcagctct
      721 gatgcacttt gtacactgga aagtcctttt tgccatcatt gcgattatgg gttttatctc
      781 atttgttggc ttactgctgg cgatgccaga gacggtgaag cgcggcgcgg tttcatttag
      841 cgccaaaagc gtcttgcgcg attttcgtaa tgtcttttgc aatcggcagt tcctctttgg
      901 tgcagcaacc atctctttaa gctatatccc aatgatgagt tgggtggctg tctcgccggt
      961 gatcctgatt gacgcaggcg gcttaacgac ctcgcagttc gcctggacac aggttccggt
     1021 gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt ttcgttaaag atccgaccga
     1081 accgcgcttt atctggcgtg ccgtacccat tcaactggtt ggcctcgcgc tgttgattat
     1141 cggcaatctg ctgtcaccgc acgtatggct gtggtcggtg ctgggcacca gtttgtatgc
     1201 tttcgggatt ggtttgatct tcccgacctt attccgcttt acgctgtttt ccaataactt
     1261 accgaaaggg accgtctccg catcgctgaa tatggtgatc ctgatggtga tgtctgtctc
     1321 ggtcgaaatc ggccggtggc tgtggtttaa cggcggtcgc ttgccgtttc atctgttagc
     1381 cgtcgtggcg ggcgttatcg tcgttttcac cctggcggga ttgcttaatc gcgtgcgcca
     1441 acatcaggct gccgagctgg cagaggagca gtgatttttg cgcgatcctg ccgtcaggct
     1501 ctattcttaa cgttatgaat aaactcattg aactcagacg cgccaaaatg ttggcgctct
     1561 ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgccg cccaattttt
     1621 gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt cggcgcgctg gcggactggt
     1681 ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat catttctcgc catacggcga
     1741 ttatcccgcg taataaagac cggattggcg aaaatctcgg ccagttcgtg caggaaaaat
     1801 ttcttgatac tcaatccctg gtggcattga ttcgacgcca cgaaccggcg ttgctgattg
     1861 gcaactggtt tagccagcca gaaaacgccc gccgcgttgg tcagcatctg ttgcagatca
     1921 tgagcggttt tctcgaactt accgatgatg cgcgtattca gcgcctgctt aagcgcgcgg
     1981 tccatcgggc gattgataag gtcgatcttt ccggcaccag tgcgttgatg ctggagagta
     2041 tgaccaaaaa cgatcgtcat caggtgctgc tggatacgct gatcgcacag ttgatcgccc
     2101 ttctccagcg cgataaatcg cgcaagttta tcgcccagca aattgttcgc tggctggaga
     2161 gcgagcatcc actgaaagcc aaaattctcc ccaccgaatg gttgggcgaa catagcgcgg
     2221 agttggtttc tgacgcggtg aattctttgc ttgatgatat tagtcgcgat cgtgcgcatc
     2281 agatccgcca tgcgtttgat cgcgccacct tcgccctgat cgacaagctg aaaaacgatc
     2341 cggaaatggc agcgcgagcc gatgccgtaa aaagctatct gaaagaagat gaagctttta
     2401 atcgctatct cagtgaattg tggggggatt tacgggaatg gctgaaagtg gatatcaaca
     2461 gtgaagattc tcgtgtgaaa gaacgcatcg cacgagcggg tcaatggttt ggcgaaacgt
     2521 taattgccga tgatgccttg cgggcgtcgt taaatggtca tcttgaacaa gccgcgcacc
     2581 gcgtcgcgcc tgagttttcc gcattcctga cgcgccacat cagcgatacg gtaaaaagct
     2641 gggatgcgcg ggatatgtcg cggcaaatag agttaaatat cggcaaagat ctgcagttta
     2701 ttcgtgtcaa cggtacgctg gttggcggtt gtattgggct aattttatat ttgctgtcgc
     2761 agctcccggc cttgttcccc ctcggcaatt tttagaaatc catgaaaaac taagtggcta
     2821 accgcgagag agatcaaata aaccatacta aaagaatggt aataatgtgg cctcttttca
     2881 ttatctcccg tggtacgggg aaggaaaatc atgtcacttg tcaccgatct acccgccatt
     2941 ttcgatcagt tctctgaagc tcgccagaca ggctttctca ccgtcatgga tctcaaggag
     3001 cgcggcattc cgctggttgg cacttactgc acctttatgc cgcaagagat cccgatggca
     3061 gccggtgcgg ttgtggtttc gctctgttcc acctctgatg aaaccattga agaagcggag
     3121 aaagatctgc cgcgcaacct ctgcccgctg attaaaagca gctacggctt cggcaaaacc
     3181 gataaatgcc cctacttcta cttttcggat ctggtggtcg gtgaaaccac ctgcgacggc
     3241 aaaaagaaaa tgtatgaata catggcggag tttaagcctg ttcatgtgat gcaattgccc
     3301 aacagcgtta aggacgatgc ctcgcgtgcg ttatggaaag ccgagatgct gcgcttgcaa
     3361 aaaacggtag aagaacgttt tgggcacgag attagcgaag atgctctgcg cgatgccatt
     3421 gcgctgaaaa accgcgaacg tcgcgcactg gctaattttt atcatcttgg gcagttaaat
     3481 cctccggcgc ttagcggcag cgacattctg aaagtggttt acggcgcaac cttccggttc
     3541 gataaagagg cgttgatcaa tgaactggat gcaatgaccg cccgcgttcg tcagcagtgg
     3601 gaagaaggcc agcgactgga cccgcgtccg cgcattttaa tcaccggctg cccgattggc
     3661 ggcgcagcag aaaaagtggt gcgcgcgatt gaagagaatg gcggctgggt tgtcggttat
     3721 gaaaactgca ccggggcgaa agcgaccgag caatgcgtgg cagaaacggg cgatgtctac
     3781 gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag
     3841 cgcctgaaaa tgctcagcca gatggtggag gaatatcagg tcgatggcgt agttgatgtg
     3901 attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgc
     3961 cagcagcaca acattcctta tatcgctatt gaaacagact actccacctc ggatgtcggg
     4021 cagctcagta cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc agtggcatat
     4081 tcgattggca ttgattccgg ctcaaccgcc accaaaggga tcttactggc agacggcgtg
     4141 attacgcgcc gtttcctcgt tccaacaccc tttcgcccgg caacagcaat tactgaagcc
     4201 tgggaaactc tgcgcgaagg gttagaaaca acgccgtttc tgacgctcac cggctacgga
     4261 cggcaactgg tggattttgc cgataaacag gtgacggaaa tctcctgtca cgggctgggc
     4321 gcacggtttc ttgcgccagc aacgcgcgca gtaatcgaca tcggtggtca ggacagcaaa
     4381 gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg
     4441 gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccaa cgtcgaacag
     4501 ctcgacagca ttaccgaaaa tgtcacgccg cacgccatca cgagtatgtg cacagtattt
     4561 gccgaatcag aagtgatcag cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc
     4621 ggagtgatta acgcgatggc gcggcggagt gccaatttca ttgctcgtct ctcctgtgaa
     4681 gcgccgattc tgtttactgg tggcgttagc cactgtcaaa ggtttaccca catgctggaa
     4741 agccatctgg gaatgccggt acaaactcac cctgatgcgc aattcgctgg tgcaattggc
     4801 gcggcggtga tcggtcagcg gcagagaaaa cgcgcatgaa agagtatcta tttttatttc
     4861 actccacggt cggcgtcata caaacccgca aggcgttgca ggcagcgggc atgacttttc
     4921 gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt tggctgacct
     4981 gtccgcctgg cgaggaaatt caatgggtga tccctgggca tacggaatca gtttattgtc
     5041 agcaggatgg tggctggcgc tgcattgcac attacgggat ttctccgcgt taaggattca
     5101 ttaagatcgc tacgtatact tctcgatatc ttattgattt gccttaaagg gattaccgtg
     5161 acaaaaagta tttcgttaag taaacgcatc ttcgttattg tgattctttt tgtcattgtg
     5221 gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc agcatcattt
     5281 cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa taatatctct
     5341 tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa acctgccgcc
     5401 attgttgaaa tgaccaccaa tggtgatttg atccgcacaa ttcctctgga ttttgttaag
     5461 gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga tgagcgcgat
     5521 tatgccattt atgtgatttc actgacccca aactcggaag taaaaatcct caaaaaaata
     5581 aaaatcccgc tgcaggaatc cccaaccaat tgcggttttg aaggattggc ctattctcgc
     5641 caggatcaca ctttctggtt ttttaaagag aaaaacccga ttgaggtcta caaagttaac
     5701 gggctattaa gtagcaatga attgcacatc agcaaagaca aggcactaca acggcaattt
     5761 actttagatg atgtgtccgg cgcagaattt aaccaacaaa aaaatacgct tctggtgctg
     5821 tctcatgaat ctcgcgcact ccaggaagta acgctggttg gcgaggttat cggtgaaatg
     5881 tcactaacca aaggcagtcg aggtttgtcg cataacatca aacaggccga aggggttgcg
     5941 atggatgcct ctggtaatat ttatatcgtt agcgaaccaa atcgttttta tcgctttacc
     6001 cctcaatcat cgcattaaca ggctggcatg cttcaccttt ttgttattaa cttgttaaca
     6061 acaaaaatga caaacaaggt taaacatgcc ttcgtccacg catcccgtag aacgcttttc
     6121 tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggtt taggccgctt
     6181 cctttatacg cctatgttgc ccgtcatgat ggcggaagga tcgttttcat ttagccagct
     6241 ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc tattttcttt
     6301 tggcgcattt caccagccat cgcgcctgcg cccatttctg ttggcttctg ccctggcgag
     6361 cgggttgttg atcctcgcaa tggcatggtt gccgccattt attctggtgt tattgattcg
     6421 cgtcctggcg ggtgtcgcca gcgccggtat gctgattttt ggttcgacgc tgattatgca
     6481 gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca ttggcatcgc
     6541 actgggcaat gaatatgttc tggcaggcct gcattttgac ctctcctcgc aaacgttatg
     6601 gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc
     6661 gaaaaaacac gccatcacac caatgccatt ggcaaaaacg gagcaacaga taatgagctg
     6721 gtggttactg gctattctgt acggcctggc gggatttggt tatatcatcg tcgcgactta
     6781 cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt
     6841 agtcggctta tcaattgtgc ctggttgctt tggctggcta tgggcagcaa aacgttgggg
     6901 ggctttgccc tgcctgacgg cgaatttgct ggtgcaggct atctgtgtgc tgcttactct
     6961 cgccagcgac tcgcctctct tgcttatcat cagcagtctt ggttttggcg gtacctttat
     7021 gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgtaccgg gaaatctcaa
     7081 tcttttaggc tttgtgacgc tcatttacgg cattgggcaa atcctcggcc cggcgctgac
     7141 cagtatgctc ggcaatggaa catcggcgct tgccagcgcc actctctgcg gcgcggcggc
     7201 gttgtttatt gctgcgctta tcagcacagt gcaattattt aagctacaag tggtcacttc
     7261 atgagggaag tttcccttac cactcaagaa actcaaccgc aaccgttgat gtcagccaga
     7321 ccccgttttc cgcttgccag aaagggaggc ctcgtttcgc catttcctgt gctttgacgg
     7381 ttaaaataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag
     7441 agagatgtac gtagtgacgc tcacctgcaa tcagcccctg tttttttatc tcgtcaagga
     7501 aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt tttttcggca aaggaaatag
     7561 cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac
     7621 taaaacgctt tttatcgctg gtggcaacca cagtatccag caatgcacgg gttaaccgct
     7681 tgcccgcttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca
     7741 gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat
     7801 ccgccagctc tttttcgttg tactttgcca tcgcgttttc gtccctggat tatctgtttt
     7861 ttatttacta tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgtaga
     7921 accttttcca cacagcgtaa ttttaccgct atgcaaaaaa cgcatagtca gaaacatact
     7981 tgcattttcc ccatacccac atggctgata aggtttttca gctccaggaa ttttcctggc
     8041 tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc
     8101 tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct
     8161 caccataatc tttttttccg gtgttttttc cgggacagac agttggtggc gcgtttttga
     8221 tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc
     8281 gtttcgcggc gcgggtggcg cgggggctaa agatggcttt ctctttgcgc tggagctggc
     8341 accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg gcggattacg
     8401 cgccgcgcag cagttaatga cgccagtgct aaaaccgttg ttgggcattc cagggatctg
     8461 ctcgctggcg ctgatcgcta acctgcaaaa caccgatgcc gccgcaggta tgaccaaaga
     8521 gctggcgcag gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac
     8581 cagcggtagc gccattatca ccaactactt ctcttccggc gtcgcggtgt ttgcctttct
     8641 gggcacgtcc gtaatcgtac ctttagcagt catcctggtg tttaaatttg tcggagccaa
     8701 cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc
     8761 atgacaactc aggtccgcaa aaacgtcatg gatatgttta tcgatggtgc gcgccgtggc
     8821 tttactatcg ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt catcattcag
     8881 gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg
     8941 ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggt
     9001 ggcgcagtcg gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc
     9061 accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc
     9121 ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt
     9181 aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt
     9241 gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat
     9301 cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagtaatatc
     9361 ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca gcgggcagat cctctgccca
     9421 ggttttattg atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg
     9481 cgcacgccgg aagtggcgtt aagtcgtctg acggaagcag gcgtcacgtc agtggttggt
     9541 ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg
     9601 ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc
     9661 actattacgg gttccgtgga aaaagacgtg gcgattatcg atcgcgtgat tggcgtgaaa
     9721 tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg
     9781 gcggaatccc gcgttggcgg tttgctcggc ggtaaacctg gcgtcaccgt gttccacatg
     9841 ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg cgatgtgccg
     9901 attagcaagt tgctgccgac ccacgttaac cgcaacgttc cgttgtttga gcaggcgctg
     9961 gagttcgcgc gcaaaggcgg caccatcgat atcaccagca gcattgacga accagtcgcc
    10021 cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc
    10081 agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc
    10141 ggtgttgccg gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agattatgat
    10201 ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg
    10261 agcgggaaag gcgaaattct gccaggcaat gacgcagact tactggtcat gacgccagaa
    10321 ttacgcattg agcaggtata cgctcgcggc aagctgatgg tcaaagacgg caaagcctgc
    10381 gtgaaaggaa cgtttgaaac ggcataaacg gttgtcagcc gcccgaatgg acgtagaatg
    10441 cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcgg ttttgcataa
    10501 tattgaaact aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca
    10561 ggcagcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc gtgcgctgga
    10621 acaggcgatt ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac tctcggaaca
    10681 aggtaaaata ttccattcgc agatccgcca tctgttgcaa cagttagaga gcaaccttgc
    10741 agaactccgt ggtggcagcg attacgcgca acgcaaaatt aaaatagccg ctgcacactc
    10801 tctttccctc gggctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc
    10861 aattgaagct attgatgtcg atgaagcggt cgataaactg cgtgaagggc aaagtgactg
    10921 tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca ttcgcttatt
    10981 tgaatctcaa ttgttccctg tctgcgccag tgacgaacac ggagaagcac tttttaacct
    11041 cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat
    11101 taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc accttttttg tctcttcgat
    11161 gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggagta
    11221 cgccatccaa caagaaattc gcagcgggca gctcgttgta ctgaatcggg acgaactggt
    11281 gatcccgatt caggcctatg cataccgaat gaatacccgc atgaatccag ttgccgaacg
    11341 cttctggcgt gaactgcgcg agctggagat tgtacttagc tgacattctc gagcgtcgca
    11401 ctacgccaac cgccattatc ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac
    11461 agcattatct caaggctgat cgccagcgtg tggctgtacg cttctttcag attgacctgc
    11521 aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag
    11581 ctctggagat gtttgtcgct ctggttatgc acttgccact cgggcatgcc tgaaacaagt
    11641 aatgacggta atgccgaatg aggagaatta aaaagagtga ggagatcgct acatattgat
    11701 ttacgcagcg atgacgtttt attgcctgaa atttcaggta aaacagcctg acgtgattgg
    11761 cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg
    11821 caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc
    11881 agtctgagac aggcctgaat cctgtcagca aagtataaaa ccttacttgc ctatgaatat
    11941 ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa
    12001 ccgctagtat attctgaata ttccgaatag ttacgtagat gaaaatactt gtcaccaccg
    12061 ctacttaatt ccggtgtgaa attatttatt tccgcataat cagccatgta ttatttgaaa
    12121 caattagttt gtaaataata taaattgata acatatcaaa tacatcaaaa ataccattca
    12181 caggcattat taatatagac atcaatatag caacatcaaa ttccaattag taataagata
    12241 tgcttttaaa ttgcatgaaa ataatttcaa atgagcgttt tttgatactt tagagtgttt
    12301 cctacccttt aatatcaccg tgtagagttg atatcactac aacagaaaca tgcatgtcat
    12361 ttgtttactt ctcattttac tacagaaaaa tttatctatt tttctaaaca tcagtgaaat
    12421 actcataaaa ggtaactatt atgtctatgc cattaagcaa tacccttcag agccaaatta
    12481 ttacagataa tcacttcctt catcatccaa aagtcgacag tgagttaaca cgtaaatatg
    12541 aacgtgcacg cctggatacg gaaaatatct atcttctgcc tcttgctcgt ggcaataacc
    12601 ataattacga tggaaaaagc gttgtcgaaa ttagaaaact tgatattagt aaagagtcct
    12661 ggccatttaa ttatgtgaca gaagcatgca gagaatccga tggtattaca acaacaggac
    12721 gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgaaatatat ggtggcatat
    12781 gtaaaaaggc gcatgctgct acagaattag cagaaggttt acggctaaat ttatttatga
    12841 agagcccttt cgatcctgtt gaggactata cagttcatga aataacatta ggccctggtt
    12901 gcaatgttcc aggttacgcc ggaaccacga taggatatat ttcaaccctt ccagtttctc
    12961 aggcaaaaag atggaccaat gagcagccaa gaattgatat ctatattgat cagataataa
    13021 ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc cgcattactt aatgctaata
    13081 ttgagatggg aaatgaccca atcattggta tagaagccta tcctggtacc gcggagatcc
    13141 atgcaaaaat gggatataaa gttatccccg gtgatgaaga tgcgccactc aaacgcatga
    13201 ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa tggaattata
    13261 ttggtaaata gccactgaga agaatgagta ctaatattca ggttatacag atatttaaac
    13321 aataccctgc aatttacaaa tcaatactca ttgcgacttc ttaatttaag aaagcgcaag
    13381 gaacgtttac ccttgcgctt attataataa gtcagacgtc gactttatcc agcggccatg
    13441 aatcaaacag atagccgtca aaataaatat cgtcaacgtt cgagaattcc agcagacgtt
    13501 gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca tcttttttga
    13561 ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat
    13621 ccagatggct gtgcaactgg atccacattg gattgttttc ccgccactgc caggactgac
    13681 ggaataactc caccagcatg ctgttatgcg ttgcttcggc aatggcgaga tggaactgca
    13741 tatcaccgct ttcgctgcta cccggcgcgc tggaagccag ttcacgctct tccagttgca
    13801 atgcctgacg cattttgacg atatcttcgc gggtagcctg caaagcggca aattccgcga
    13861 tgttactttc cagtaactga cgcgcctgta acagctcaaa aggacccgca tcattacaga
    13921 cgttggcatc cgaactgtct gtgttgtggc tgcctgagct atcaagaaca tagataccgg
    13981 caccccggcg cacttccacc agccctttga tctccagcat gatcagcgct tcacgtacca
    14041 ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggcagc cgttcgccag
    14101 gattgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc tggtaaggtc
    14161 tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt
    14221 ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta cggcccctgt
    14281 taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc aaaaaaacga
    14341 aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta atcagttagt
    14401 tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata tgccgccgtc
    14461 accgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc gctcaggcct
    14521 agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt taatcttctg gaactcggcc
    14581 agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg ggtgtaacgc
    14641 atccagccag ccacgcccag cgccaggtga cgccagctgc cgccgttttg caggtgcaga
    14701 cgcaccgggt ccagcagacg ctgcggtaac ttctggctgc cgtccatcgc aatctgccag
    14761 gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc ataggcgttc
    14821 aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat cagggcaaag
    14881 gccgctttgc gataatccgg gttagtcatg gtgtcggcaa tagtttcata gccgccgagg
    14941 taaccgaggt acgccaggaa agagtggcta ccgttcagca tacgcagctt catcatttcg
    15001 aacggcacaa cgtctgcaac gaactgtgcg cccactttat cccagtccgg gcgaccatta
    15061 acgaaattat cttcaatcac ccactgacgg aacggttcgc aggcaatggc gcacgggtcg
    15121 taaacgccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg
    15181 cggtcaacca tggtgcacgg gaaggtgacg ttctcttcaa tccatgccgc tagctgcggg
    15241 tcacgcgcct gagccagccc cagtaccgcg accttcgcca catgaccgtt ttcacgcacg
    15301 ttatcgcagg acatcaccgt aaatgctttc agcccttttt cacgacgcag acgcaaggct
    15361 tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc gtgcttgatc
    15421 agcgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg
    15481 accgtcagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat accttcgcag
    15541 ccgtcgattt ccgggtgcag cgcttctttc atcgaaccga taattttcag ctcggtgctt
    15601 tccgcgcctt tttccgccac ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc
    15661 acgcggtcgt tgcctggcat caggttaact tcgcagatac cccagtcgct gtcggtgctt
    15721 tccagcagat gatgggtgta cagcgcctgg tgcgcacggt gaaacgcccc gcaaccgaga
    15781 tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac cggcagattg
    15841 ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcagagagt gacggcaagg
    15901 aagaaggaac cggcacggcg cgcagccatg ccggtggata ttaacggcta aagaaagcgc
    15961 gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga ccaattgcgg
    16021 agtaacctgg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg tggtccggac
    16081 gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct tcaacaatcg
    16141 ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt
    16201 tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact
    16261 gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg
    16321 tgaaaccgtt cgccatgcta tttacggtat caaccatcca ctgcatatct tcaatggtag
    16381 aaacaatgcg cggcaggccg aggattgggc gcggcggatc gtccgggtga acagccatac
    16441 gcacgccaac ttcttcggca accggaataa tcgccttcag gaagacggca aagttttcgc
    16501 gcagtttggc tttgtcgata tctttgtaca gctccaggtg cttacggaac tggtcgaggg
    16561 tgtacccttc ttccgcgccc ggcagaccgg caatgatgtt acgggtcaga cgcgctttgt
    16621 cttcgtcgct catagtggcg aagcgttcag ctgcctgagc aatttcttct tcggtgtaat
    16681 ccgcttccgc acctgggcgt ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt
    16741 cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta cgggtccagt
    16801 cgagcaccgg catgaagttg tagcacacgg tgcgaatacc gcactgcgcc aggttgcgca
    16861 gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt ttgatatctt
    16921 cgtgaattgg cacgctttct acgacagacc acaccaggcc tgcatcttca acgatagcct
    16981 tgcgtttgag gatctcttct acggaccata cttcaccgtt cgggatatgg tgcaaagcgg
    17041 taaccacgcc agtcgcgccc gcctgacgga catcagctaa agaaaccgga tcgtttgggc
    17101 cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg ttccacagca
    17161 aagagacgcg gaaccctgga gttgtttagg tatccggatg aacaaccaga ccgcagccgt
    17221 ggcgttgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca tgtcaacccg
    17281 aatcctgaaa ttggttaacc acatcacaag aatttcactc tcctggttgt tgctgcaaat
    17341 tgcagcaaaa ggtaatcgat ggttattgat cagaggatgt gacattcatc gcaacaatgg
    17401 ttgaccaatt tacataacat gtcgcccaaa ataacaacgg ttcaaccagg taaggtaaaa
    17461 cgttatgcat gtgcttaaca ttctctgggt ggtattcggc attggtctga tgctggtact
    17521 gaatttgaag ttcaaaatca attcaatggt ggccttgttg gtggcggcgc tgtccgtcgg
    17581 gatgctggcg ggcatggatt tgatgtcgct gctgcacacc atgaaagcgg gcttcggcaa
    17641 cacgctgggg gaactggcta tcatcgtggt gttcggtgcg gtcatcggta aattgatggt
    17701 cgactccggc gcggctcacc agatagcgca tacgctgctg gcacgtctcg gtctgcgtta
    17761 tgtacagctg tcggtgatta tcatcggcct gattttcggt ctggcgatgt tctatgaagt
    17821 ggcctttatc atgttagcac cgctggttat tgttattgcc gccgaagcta aaattccgtt
    17881 cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac tgttcccacc
    17941 gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg gatatgggga tggtttatat
    18001 ctatggcgta ctggtgacga tcccaagtgt catctgcgca ggtctgatcc tgccgaagtt
    18061 cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac cggtagatat
    18121 gaataatctg ccctctttcg gcgtttcgat tctggtgccg ttgatcccgg cgatcattat
    18181 gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcgt gggaagtggt
    18241 taactttatc ggttcctcgc cgattgcaat gtttattgcg atggtggttg cattcgtact
    18301 ctttggcacc gcgcgcgggc acgatatgca gtgggtaatg aacgcttttg aaagcgcggt
    18361 gaaaagcatt gcaatggtga ttctgatcat cggtgcaggt ggcgtgctga agcagaccat
    18421 catcgacacc ggcatcggcg acaccatcgg catgctgatg tcccacggca atatctcgcc
    18481 ctacatcatg gcatggctga tcaccgtact gattcgtctg gcgacgggtc agggtgtggt
    18541 ttcagcgatg accgccgccg ggattatcag tgcagcaatc cttgatccag caactggtca
    18601 gctggttggc gtgaatccgg cgctgctagt actggcgacg gctgcgggtt ccaacaccct
    18661 cacccacatt aacgatgcct cattctggtt gttcaaaggt tactttgacc tgtcagtaaa
    18721 agacacgttg aaaacctggg gtctgctgga gctggtcaac tccgtggttg ggctgattat
    18781 tgtgctgatt attagcatgg tagcgtaaaa gagcacctga gcctgccatt ggcaggctct
    18841 gttcggattg tcggtaaagt gcgctttgtt tatgccagat gcgacgctga cgcgtcttat
    18901 ctggcctaca aagggctaac gtgcaggttt tagcttcagg taatattgcg tacctgcatt
    18961 agcaatgccc tgtgatttct ttattgataa acaaaagtca cggcaataat cgattgcaca
    19021 ttccctgcag tcacctgccc tccggtacgt gcgtaatttg ccgttaatcc cagacttacc
    19081 gccgaagtcc ctactgctcc taacgatacc gtgttattcg ctggaataat cgtaccgttg
    19141 cgcgtcaact gtacgccgac gccctgtgca ggtgaaaacg acgcggtatt ggtgaaaatc
    19201 gagttgcccg catctgcggt tgtgccggag aggtaatacc ccaggttttg gcttttcgca
    19261 caataaacgg taagaggaat cggcactgaa ccagggtagt ccggcagagt aacggtgaca
    19321 tcacgagcag aaacatcgca gccgccagtg ggcaccacca catcattatt ggcgtaaata
    19381 ttccacacaa actggaaatc atcgctgtta tagttgttgg tctgtcgcaa aataagcacg
    19441 gcaattaatg agccagcttt aatcaccacc ccgcccgcac tgctcacagg cgtcaaataa
    19501 agcgccaccg gccacggctt atccgttctc gaattataaa caacccgcgg cgtttcactg
    19561 gtggtcggaa atggatagct actgccacta tattttacgg tcccggaaaa attagataac
    19621 acgccgccat aagccgagcc tcgttgcagt gtgacataat ctgtaatggt ttccgggtaa
    19681 tcgttatggc aaaagatttg cgtcgaaaga tccacgacca ggttttgccc cacattcacg
    19741 gcgggcgcaa ggtttacata aacattggcg ctgccaccgc caatagggat agcggtgcca
    19801 ttggcggttt tacaggcgaa tgaccaggca tttaccgacc agcccatcag cagtacagca
    19861 aacaggttaa taactcgttt cattacaatc atctcttcgg cttcagctgt aggtataggt
    19921 gatgctaatc actgcctgaa tggttccctg agtggctccg ccatttaccg tcaatgctct
    19981 gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc acctgaactg ttttggttgc
    20041 gccactattc aatgtgttgc cactgtcatc ctgtagctct aactggatgt tttgcgcggt
    20101 cccctggttt ttataatatc cggtactgtc ggctgccccg ctgaagctgg cagtgaccct
    20161 cgacgttccc accggacaat tagtcaactc aagcgcaaca tcatgccagg ccgatgccgc
    20221 cccggcagac atcagactga aagaataaag atcgccgaga tctaccgtgg cattggtggt
    20281 ggaaaccgtg cacggtttgg cgacgacctt accgttcacc gtgatggtga catcggctgc
    20341 ctgtatcgtc gcacttgcga gcgccaatat tgccgccaat acatacccac gtttgcacca
    20401 tttcatgagc ctctccagtt actgatattc aagagtgaag gtagccgtgg cattgatatg
    20461 ccccgcagtg acaggcacct gtgtcgccat tagccgggcg taaaaattca gcgtatttgg
    20521 tttacccggc gtcagggtcg tccacgaaat cgcggacgat ggggcattaa tgggtatctg
    20581 attttgttgc tcattcagaa gctgtattcc cagtcccgca accgctgaca ccgtattttc
    20641 aagtgcaagc aggttggcat tgtggctatc tgcaacaccg gtaaacccaa cttttacggc
    20701 agaaacggca ttaccacagg gtgacagcaa aatacgaaat ggaacgacag gagtcgtcgc
    20761 gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa aattggttga
    20821 ttcagcggcc acactacagc cgttatctct gacatagccg cggatagtaa tcgtgctatc
    20881 cgcagccaaa gcgtgactta ccgccagcca caaaaaagcg cacagaagat aaaaaggttt
    20941 gtttctcatc acgccccctt aacgacattc agctgatagc tgagttaata actgctgctg
    21001 actctctggt ggcagttgat aattggcgac acagtgagca ttttcctctt ctccccattt
    21061 cacctgaact tttcccgcta aaggcattcc gctgaggtaa acctgaccat tatccgcaac
    21121 aatgccgcta ctctggctac tctctgatgt caccatcgcc ccaaacggca gcggcttatt
    21181 attgtgggtt agcgtcatga gcagttttat cccaacgcgc gctttaaact ctgctcgcac
    21241 gatcgcccca cgggtgggaa caacgttagc gaccgcgtta tctaaatcga cgttatcagc
    21301 cagggtattg gtatccagcg ctactctgtt ctcccgatat tcagtggcat aaggcagcac
    21361 ggcataaccg cgccagtcgg tacgcacccc cgtctggttt tcgacttttg catcttttgc
    21421 gccaggcgct ttaacaagca ccaccgtatc gtttaacggc tgccccagcg ttacgccatt
    21481 ggcatgagcc agtaccccac cgctgactcc gtaatagagc tgcttaatat catcgctatg
    21541 gctgtaaccg atgttggcat tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt
    21601 acttccgcta ttaccatcac ctcccccggc gtaaccggtt tgcacgctat agctgaggtt
    21661 gttgtcttcc agcaacgtac cgtatacacc gaccagattg gtcatccgac cgttgagatc
    21721 gtgtgacatg ctgtaactgg cactggcatg tcgccactga gatttactgt cagaacgcag
    21781 ccagtggctg aaaggaatat tgacgttaag cgctaacatc tgatcccgtc ctttttgcca
    21841 ggcgtttttc gtcaggctat agctgagcgt ccagttgata tcttcgaatg cagtatttaa
    21901 accagcctgg aattgctcat cgacattact cgttccccaa taagtttgat ggctaccact
    21961 caaatacagt gttgatgtgc gcccgagttg ctgagtaacg gtgagttgta atttcccgcg
    22021 tttgttataa gcgagattgt aatagtcggt gaatttcggc ttaacctgaa taacaccatc
    22081 ctgtgtttcg atgttgtagc cattcattcg actgtatgtt gtatcagcga aattaaaata
    22141 tccgctggtc gaataacggt aacccactaa ctgaatattc gtgcctgatt cattgagcga
    22201 tttgttatag agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga
    22261 attagcctgc gtcatatcca cagacagagc gcccagtgcc cccatgtttt tcccgatacc
    22321 gaaattaaac gcacgataac gatccgccag ttgcgttcca ccatatattg tccagccagc
    22381 cggaaggccg tggagtaatg tactctggaa aaagcggggt ttttcctgct gcgcatttcc
    22441 actacggtat tctcctgccg taatggaata acgagtatgc ccttcacgtt gcaaaagcgg
    22501 gactgacgaa tagggtacgg taaagatctg cgtgctgccg tcagcctctt taatcgttac
    22561 ctgcaagtca ccactattac ctgcggcata gatatcgttg atggtaaaag gccccggcgg
    22621 caccgtacta ttataaatgt catacccatt ttgtttaata gtgacctgtg cagtaccacg
    22681 agcaataccg tggatcaccg gagcaaatcc tctttggcta tcgggtaaca tattgtcatc
    22741 tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa atatcgccct gggtataacc
    22801 atcacccagc gtcagccggg aacgtaacgg aattatgtct cgctcaagcc aggtattgat
    22861 atgctgccat ttatttttgc tacctgatga actgtcgcta ctgttataac tccaggtggt
    22921 attgtcgcgt aaacgccacg caccaatatt taacccactc tgtaggttta aatatgcata
    22981 atggctgtta cccccaatcc gattctgtac actatttccg ctgaaattat aattgagcaa
    23041 tccggcatta ataccaggat cccataactc aggaggaata taaccacgcg cgcgattact
    23101 cataaatgcc tgagggatcg tcaggttgag tcgctgctga ccaacatcca gatgcgcagt
    23161 agcgtcatgg atcattgagg ttaatggcac gcaggcatca tccgccagca gattcatacc
    23221 ggagacagaa gccgtattca gctccatact tgcaagttgc gcgcgtgtca ggcagggaac
    23281 aatcccttgt tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca tataaccatt
    23341 attcaaatag atatcgacac gatacgtccc tggcggtaat tcttgcccat tttcaaaacg
    23401 cgataaatcg gccacagcct ggggatcatc cgctaaaaag cgcggattaa aatagagttc
    23461 ggcagatgac aaaggtgcct gtgcggcaaa agcacaggcg acaaagagcc ggacaaaaaa
    23521 accagccaaa cgatgcttac gaatatgcaa gcattgtgtg tttcgctggt aaagtcttaa
    23581 attcagatat gacattattc ctccggttta aaatcacccg gcagtcaatt ctttttattc
    23641 aggcgaaaaa ttccccctgc gttattccat tacgcccgtc attttggggg taagtgcgcc
    23701 ataatcattt attgttcggt aagtaatatt gcttcctgca tcagaaggca atttaaccgt
    23761 gctttcgccc attggaggca ccaatgcatt ttcaagaacc cgggttccgg cattcaactc
    23821 tgttaccgtc aggtaatagg gtgtcgggtt aatcagagtc agagaattcg cgctacgacg
    23881 aaatcttaat ttttctgcgg cctgatcggg tggcaacgct aatttagccg ggcgatagta
    23941 cagtttaatg cggctgataa ttgcgagctg tagcgtattc tcagtcaatt ttgatttatc
    24001 cattgacgga atcgctttaa cgttcatcca gaataaactt tcccgatcct gtggcaattg
    24061 gttatttgtt gcatcaagaa tacgtaaggt attctctttt tttcccttca tcgcaaacag
    24121 aggtggcgtc acgataaaac gaccatcctt tacaccatcg gcattttcca cccatgattg
    24181 aattaaatag gtgctatttt catcattatt tgtcacggca agttgcactt gtttttgccc
    24241 tgccggataa attacgcgag tcgcacctaa ggccactccc gcttcggcgc gtccggcaac
    24301 catcattgcc atgaacatca ggatacctgc cagcaagcag aatgttattt cctgcgattt
    24361 ccttacattg acgtttttat tactcactgt cctgttcctg ttatcacatt atctgctgaa
    24421 caattactga taggttaaag agaaccaggc ctgggcattg gcgatgccgc cagtaacccg
    24481 acgcccggta gcacgatatt tggcgatgaa atgtagcgaa gtagagcctg agtaaagccg
    24541 tttccagttt gctggaggac gattaatcgg tacgaggttt ccttcatcat caaacaacgc
    24601 tacgccaata ttggtggcta tccctggccc ctctcccacg gaaagcacat ctggattttt
    24661 accatccgcg acaccgtgaa acgccacacc cacacgttca ctcaccaccg tgctacattc
    24721 ccgtaaatga ataacaaaag gcaccggtgc gctatcttcc cctaccgcat gaaaccggtt
    24781 actgctgatt tgccccatat tgaccgtcat ttgtttatca ccggcttcaa tccggcaagt
    24841 ttccgcaata atgacgccct gaaattgcat atttccgccg ggcagcgtgg tgttccattt
    24901 atttccggcc agagcaaaca ttggcagcaa cgacgctaat agaaataatc tcttcctttt
    24961 catcgttatt tttatcgcac aagggtgggc atccctgccc gtaatgacgt ccctgaacct
    25021 gggtaggtta ttgatactga accttgaagg tcgcatccgc attagcagca cccggggttg
    25081 cctcgccgat tgcataataa cgcgcctgga acggaatggt gttggtaccg ttattcaggg
    25141 ttgtttgtgc actaaatgtc gcaccgtcca gcgtcagctc atttcctgtt ctgtccagta
    25201 tctgcacacc aacatttgtt gcgctacccg cagctgaact ctgcagagcc agtactttag
    25261 gatgagcact gtcaatcgca gtccccaaga aggcaacagc ggcttttgtg gcaacactgg
    25321 tatcgcaatc attcagctga atgttaaaac cgacagcaga gctggttgct ccagtctgct
    25381 tcagactagc ggtacgaacc tggcccaact gaacggtttg atcaacagag cctgcatcaa
    25441 ctgcgcaagc ggcgttaaca acttcccctt taaagtgaac ggtcccacca tttaccgtca
    25501 tgggtgtagt ttcagccaga gccgctgtag aactcaggga cagagccgac agaacaacaa
    25561 ttgccagagt tttaattttc atactgcttt cctttcaaaa aactatttct aaatcgacat
    25621 gggcagtcgt tccgtacact ttgttttgtc aacgagtttg actgccaaca ctgcacagtt
    25681 tccccccaaa agatgaaaca tttggggcca ttttgactca tagaggaaag catcgcgcac
    25741 aaactttttc agtttatttg ttggctttat attctattgt tatctttatt tatagatttt
    25801 tatattgcat gaggtggttt ttggagagaa gaatgaggaa gttgcttcga gacacagaaa
    25861 cgttagcttt acatttagcg gaggtgatgt gaaattaatt tacaagagaa atgatttaca
    25921 tatcaaacag ttagatgctt tttgtcgttt tataatattt ttatgcttga gaaaaaatac
    25981 gtaacttatt tatgatatgg a
//
LOCUS       DAZFZC010000075        25546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFZC010000075 DAZFZC010000000
VERSION     DAZFZC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.075"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            218..637
                     /locus_tag="ACHKXP_003424"
     CDS             218..637
                     /locus_tag="ACHKXP_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7648368.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            637..1905
                     /gene="umuC"
                     /locus_tag="ACHKXP_003425"
     CDS             637..1905
                     /gene="umuC"
                     /locus_tag="ACHKXP_003425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO7648369.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1951..2481)
                     /gene="dsbB"
                     /locus_tag="ACHKXP_003426"
     CDS             complement(1951..2481)
                     /gene="dsbB"
                     /locus_tag="ACHKXP_003426"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO7648370.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2627..4168)
                     /gene="nhaB"
                     /locus_tag="ACHKXP_003427"
     CDS             complement(2627..4168)
                     /gene="nhaB"
                     /locus_tag="ACHKXP_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO7648371.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4389..5108
                     /gene="fadR"
                     /locus_tag="ACHKXP_003428"
     CDS             4389..5108
                     /gene="fadR"
                     /locus_tag="ACHKXP_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO7648372.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5160..6692)
                     /locus_tag="ACHKXP_003429"
     CDS             complement(5160..6692)
                     /locus_tag="ACHKXP_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO7648373.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7022..8320
                     /gene="dadA"
                     /locus_tag="ACHKXP_003430"
     CDS             7022..8320
                     /gene="dadA"
                     /locus_tag="ACHKXP_003430"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO7648374.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8330..9400
                     /gene="dadX"
                     /locus_tag="ACHKXP_003431"
     CDS             8330..9400
                     /gene="dadX"
                     /locus_tag="ACHKXP_003431"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO7648375.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIE
                     RIWSAIGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9786..11522)
                     /locus_tag="ACHKXP_003432"
     CDS             complement(9786..11522)
                     /locus_tag="ACHKXP_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO7648376.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQHHESNISWMFIVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFSLTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11617..12531)
                     /gene="ldcA"
                     /locus_tag="ACHKXP_003433"
     CDS             complement(11617..12531)
                     /gene="ldcA"
                     /locus_tag="ACHKXP_003433"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO7648377.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12631..13242
                     /gene="emtA"
                     /locus_tag="ACHKXP_003434"
     CDS             12631..13242
                     /gene="emtA"
                     /locus_tag="ACHKXP_003434"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO7648378.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPLWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13244..13978)
                     /locus_tag="ACHKXP_003435"
     CDS             complement(13244..13978)
                     /locus_tag="ACHKXP_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO7648379.1"
                     /translation="MSHYHEQFLKQNPLAVLDVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14179..14433
                     /locus_tag="ACHKXP_003436"
     CDS             14179..14433
                     /locus_tag="ACHKXP_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7648380.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14611..15051
                     /gene="ycgY"
                     /locus_tag="ACHKXP_003437"
     CDS             14611..15051
                     /gene="ycgY"
                     /locus_tag="ACHKXP_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO7648381.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15130..16827)
                     /gene="treA"
                     /locus_tag="ACHKXP_003438"
     CDS             complement(15130..16827)
                     /gene="treA"
                     /locus_tag="ACHKXP_003438"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7648382.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17147..18565)
                     /gene="dhaM"
                     /locus_tag="ACHKXP_003439"
     CDS             complement(17147..18565)
                     /gene="dhaM"
                     /locus_tag="ACHKXP_003439"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO7648383.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQVRYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18576..19208)
                     /gene="dhaL"
                     /locus_tag="ACHKXP_003440"
     CDS             complement(18576..19208)
                     /gene="dhaL"
                     /locus_tag="ACHKXP_003440"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO7648384.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19219..20289)
                     /gene="dhaK"
                     /locus_tag="ACHKXP_003441"
     CDS             complement(19219..20289)
                     /gene="dhaK"
                     /locus_tag="ACHKXP_003441"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO7648385.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVTG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVNVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20517..22436
                     /gene="dhaR"
                     /locus_tag="ACHKXP_003442"
     CDS             20517..22436
                     /gene="dhaR"
                     /locus_tag="ACHKXP_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO7648386.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22536..25403)
                     /locus_tag="ACHKXP_003443"
     CDS             complement(22536..25403)
                     /locus_tag="ACHKXP_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7648387.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMTMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         6287 a         6352 c         6615 g         6292 t
ORIGIN      
        1 attatggcga atgcttctat tctatttttt tgccgggtga tatttttcat ttctgctgga
       61 tgagcgtcgt cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc
      121 tatgcagcga caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacta
      181 ctgtatataa aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga
      241 tctccgcgaa attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc
      301 accggcagca gattacgttg aacagcgcat cgatctgaat caactgttga tccagcatcc
      361 cagcgcgact tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga
      421 cggtgattta ctgattgtcg atagcgctat taccgccagc catggtgata ttgtcatcgc
      481 tgctgttgac ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat
      541 tcccatgaac agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt
      601 tggtgtggtg atccacgtcg ttaaggcgat gcgctgatgt ttgctctctg tgatgtaaac
      661 gcgttttatg ccagctgtga gacggtgttt cgccctgatt tatggggtaa accggtggtt
      721 gtgctatcga ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggt
      781 gttaaaatgg gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt
      841 tgctttagca gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg
      901 gaagagctat cgccccgcgt cgagatttac agtattgatg aggcattctg cgatctgaca
      961 ggtgtgcgta attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta
     1021 caacgtaccc atcttactgt tggcgtgggg atcgcccaga ccaaaacgct ggctaagctt
     1081 gccaatcatg cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat
     1141 ctggaacgcc agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga
     1201 cggcggatca gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat
     1261 acagatatcc ggtttatccg taaacatttt aatgtcgtgc tcgaaagaac ggtgcgtgaa
     1321 ctgcgcggcg aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc
     1381 tgttcccgct cgtttggtga acgcatcacg gattatccgt cgatgcggca ggccatttgt
     1441 agttacgctg cccgggcggc ggaaaaactt cgcagcgagc atcaatattg tcggtttatc
     1501 tccacgttta ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcgcg
     1561 tcggtaaaac tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga
     1621 tctctggatg ccatctggca agcgggccat cgttaccaaa aagcgggcgt gatgctgggg
     1681 gatttcttca gtcagggagt cgcgcagctc aatttattcg atgacaacgc accgcgcccc
     1741 gggagtgagc aattgatgac ggtaatggat acactgaatg ctaaagaggg cagaggaaca
     1801 ctctattttg ccgggcaggg gatccagcaa caatggcaga tgaagcgagc catgctttca
     1861 ccacgttata caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa
     1921 aaaagcgctc ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc
     1981 tttaaacggc tgggaaatca ccaccagcac tgcgacaatc aggtaagcga taaaaatacc
     2041 gagcagccac tgcggcattt ccagacctaa aaaatcccac tgacgctcgg cgcaatcgcc
     2101 agaggcgaca aacacttgcg gcacccactt atccagcggc agccattccg ggaaacgaac
     2161 cataaaatca caggtggcaa acggcgaagg atagagctga agcatggtgt gctcgtaagt
     2221 taactgcaca ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg
     2281 agttttcggg gcgatcgcgc caatcagcgc cgcacccaga acgccgaata acgcgcagcg
     2341 ttcataaata cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt
     2401 cagttccagt gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca
     2461 ttggttcaaa aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa
     2521 accaattcat tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat
     2581 cgggcgaaaa ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta
     2641 tccagcccat ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca
     2701 agccgacgag tgtcaggacg agggtgtaag gcagggccat ccacaccatg cggccataag
     2761 agaggcgaat caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt
     2821 tcggcgtagc gacggagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt
     2881 attgcttcaa cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg
     2941 tccccacgaa gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg
     3001 acagctgagc atgttccgat gcctgcaaca caaactgaat aattggcgaa aacagttgtt
     3061 ggtcgataat caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag
     3121 attcggtgaa ggctttaccg atagcatgct catcggtgac accggtcaat gatgttgcca
     3181 gaataatgac tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca
     3241 ccagccagac gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct
     3301 ggtggcggct ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt
     3361 caccgtagcc aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca
     3421 gaaccggaac ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg
     3481 ctttagcgat gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg
     3541 tgccgacacc ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca
     3601 ctttgtaatg cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag
     3661 aggctacgcg gtgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga
     3721 cggttaaggc atcgaggaac gcggagagga acgcagccgc cacgcaaaaa gagagcgaca
     3781 gcagcatttt ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt
     3841 tcataaaata gatacccgcc accataaaca tcagtaacag caagacttca agatttgccg
     3901 ccacctcttc acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca
     3961 acagaccacc ggggagcagc gggtagcatt tcagggccat cgccagagtg aaaataaatt
     4021 ccgctaccag caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga
     4081 ttaagaaaat gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc
     4141 atagcgcgcg gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg
     4201 atgtttttat gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata
     4261 gtcaagggga aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc
     4321 tatcagcgta gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa
     4381 atctcactat ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg
     4441 aaagtatctg gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt
     4501 cagaattaat tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag
     4561 atggctggtt gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt
     4621 ccggtttaaa tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta
     4681 ttgataattt gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc
     4741 gtcagcatcc cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg
     4801 ccgatgcctt tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca
     4861 acccgattta cggtctgatt cttaacggga tgaaagggct gtatacgcgt attggtcgtc
     4921 actatttcgc caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg
     4981 cgttgtgcag tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg
     5041 agagtggcga gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg
     5101 ggcgataatc ccttccgttt aaagagcaaa cccctcaaac gaggggtttt ttgttgtttt
     5161 tacagatttc ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt
     5221 tgctgttcga gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg
     5281 cgcccccgat ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg
     5341 ccgcgcaaat ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc
     5401 gacgataacc ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc
     5461 agataattat gccgatcgtc atccagcacg gtgaagaagc ggaaatcacg catcactttc
     5521 ggtgacagga actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc
     5581 gtttccagcc agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc
     5641 gactgacaaa tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg
     5701 ccgctgtacc acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa
     5761 aactccagca taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggtg
     5821 tagtgccaga aggtcgccca gccttcgttc atcacctgag tctgtttttg cggataaaaa
     5881 tactggctca ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc
     5941 gcatttttct ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct
     6001 tcagcaaccg ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga
     6061 ctttgcagat actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt
     6121 tgcgggcgtt tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc
     6181 cgttctactt catcaacgcc ataacgctct tcgcactcgg taatatattt acgggcaaaa
     6241 atcagataat cgacaatcga actggcgtcg gtccagctac ggaataagta attgtttttg
     6301 aagaaagagt tatgcccata gcaggcatga gccatcacca gcgcttgcat ggtaatggtg
     6361 ttctcttcca tcaggtaagc gatacacggg ttagagttaa tgacgatttc ataggccagt
     6421 ccttgctgac cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag
     6481 tgcggatagt taattggcat gccgacgctg gagtaggcat ccatcatctg ttctgaggtt
     6541 atcacttcaa tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc
     6601 tctgccagat aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg
     6661 gtgtccttat tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct
     6721 ctgtgtggag cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa
     6781 tttttttctt tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt
     6841 tatgctggat aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt
     6901 cagggagatg tgagccagct caccataaaa aagccgtatg ttgaataata ttttcaactg
     6961 agttatcaag atgtgattag attattattc ttttactgta tctaccgtta tcggagtggc
     7021 tatgcgagtt gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa
     7081 tcaggcagga catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag
     7141 tgccgcgaat gccgggcaaa tctcccccgg atatgctgca ccgtgggcgg caccaggtgt
     7201 gcctttaaaa gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga
     7261 cggtacgcag ttccagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca
     7321 ctacatggaa aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa
     7381 agcattgcgc gccgaaacca atattcagta tgaagggcgc cagggtggga cgctgcaact
     7441 gttccgtacc gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc
     7501 cggcgtaccg tatcagctgc tggaatccag ccgcctggcg gaagtggagc ccgcgctggc
     7561 agaagtggcg cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg
     7621 tcagctattt acccagaatc tggcgcggat ggcggagcag gcgggggtta aattccgctt
     7681 taatacgccc gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtgtgg
     7741 cgatgaagtg attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat
     7801 gctcaaaggc attgttgata ttccggtgta tccgctgaaa ggctactcgc tgaccattcc
     7861 aattgcgcag gaagatggtg cgccggtatc caccattctt gatgaaacct acaaaatcgc
     7921 cattacccgt ttcgataacc gcattcgtgt tggcggaatg gcggagattg ttggttttaa
     7981 taccgagctg ttgcaaccgc gtcgtgaaac gctggagatg gtggttcgcg atctctatcc
     8041 acgcggcggt catgtcgagc aggcgacttt ctggactggt ctgcgcccga tgacgccaga
     8101 cggcacgccg gttgtcgggc gtacacgctt taaaaatctg tggctgaata ccggtcacgg
     8161 cacgctcggc tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg
     8221 tcgcacgcca gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac
     8281 gccatcacgt ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc
     8341 gatacaggcc agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc
     8401 cgcgacgcac gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga
     8461 gcgtatctgg agcgcgatcg gggccaccga tggctttgca ttgcttaacc tggaagaggc
     8521 aataacgtta cgtgagcgcg gctggaaagg accgatcctg atgctggaag gatttttcca
     8581 tgctcaggat ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg
     8641 gcagctcaaa gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt
     8701 gaacagtggg atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg tctggcagca
     8761 gttgcgggca atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ccgaagcgga
     8821 acatcctgat ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga
     8881 gtgtcggcgt tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga
     8941 ctgggttcgg cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat
     9001 cgccaatacc ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac
     9061 gctaaaagcg ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg
     9121 aatcggcatt gtcgccgcag ggtacgccga cggttatccg cgccacgcgc ctaccggtac
     9181 ccctgtttta gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct
     9241 agcggtcgat ttaacgcctt gcccgcaggc gggtattggt acgccggttg agctgtgggg
     9301 caaggagatc aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat
     9361 gtgcgcgctg gcgctgcgcg tcccggttgt gacggtgtaa cttgttgtaa gccggatcgg
     9421 aggcaacgtc ttctgggtgc aaaaaaatca tccatccggc tggtcagcaa ctgtagttgt
     9481 taatgtgaca gagccattgc ccatgatagt gtccattaaa aggatggaca ctatttcccc
     9541 ggaacctgaa ctcaccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc
     9601 gaactcgact tcagataaat cacgctttac ccttgatgga gcctgtacat agatttgtgt
     9661 aattgcctga ttttgatatg ttcaattcaa catcaaatga aggttaaatt atggacgaca
     9721 aacaattgca ggctcaggct gcgttcagca aagcatcgca accggcgata gatgcttcat
     9781 taaatttaag attcagcttc ctcttcagcc acccgtacgc caatcttcaa cacttcatta
     9841 tcttctttct cggccaccgt ccagatcatc ccggcaaact ctacctggtc accgacaacc
     9901 ggtgccgcgc ctaacaactg ctggacaatt tcacccagcg tttgctgctt atcacgatat
     9961 tctcgcccgt cttccaggcc atatatcagc gcaacatcag catatttggc gctggcttcg
    10021 agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg gcggcgactg gctgaacagt
    10081 ttaccgagcg ccggaagatc gcgttcccga ccaattacac acaacacatc gccttcgcgc
    10141 agtcgggtgc tgccggtagg atgaagcaac tggttatcac gaaacagtgc cgcaatacgc
    10201 gtctcttttg gcatatgcaa atcgcgcagt gccgcgccca cgcaccattt atcggcactc
    10261 aattgataaa caaactgctc ccacggattt tccggatgaa tatccaggcc aacgcgtgac
    10321 accggacgtc ccactggcgg aaccaccact ttggcttttt tagccgccca cgagagtgat
    10381 gttccctgca agagcagtga aaccagaacc acaaagaagg cgacattaaa gaacagacgt
    10441 gcattctcca gccccgccat catcgggaac acggccagga tgattggcac cgcgccgcgt
    10501 aatcctaccc agctgataaa cacgcgctca cgcagattga aaccgcggaa ggggagcaat
    10561 ccagcaaata ccgaaagggg acgggcgaag aatatcatcc atgcggacaa aatgagcgcc
    10621 ggaatggcaa taggcagcag atcgcttggg ttaaccaaca gccccagcac caggaacatg
    10681 gcgatttgcg ccagccaggc gaggccgtcg aaattttgca ggatgccgta gcggttgcga
    10741 atagggcgat tacccagcag aaaaccgcac agatacaccg ccagaatacc gctaccttcc
    10801 agcgcagtag ttaatgagaa aatcagaata ccgccactta atgccagcaa tggatataat
    10861 ccggcgggca gggcgatgcg gttgatcatt tgcagtagta aataaccgcc gccaagacct
    10921 attacaatgc ccagaccaaa ttgttgcaga atatcgacaa tgaacatcca gctaatattg
    10981 ctctcatgat gctggatcat cgcaatcagg gtaatcgtca gaaagaccgc cattggatca
    11041 ttactgccgg attcaatttc cagcgtcgag ccaacacgtt cgttaagccc cttaccaccc
    11101 agcagagaaa agaccgctgc agcatcggta gagccgacga tagcgccgat taataagcct
    11161 tcaatcaaat caagattaaa cagccacgcc gccatcatgc cggttaaacc agaggtgata
    11221 agcacgccca gcgtcgccag cgacagtgcc ggtcctaacg ccacacgaaa ggaactggcc
    11281 tgagtgcgca tcccgccgtc gagcaaaata atcgccagtg ccaggttact taccatgtag
    11341 gcgaaggggt aattatcaaa cgggataccg ccgacgccat cgactcctgc cagcatgccg
    11401 atcgccagaa aaataaccag aataggaatg ccaagacggg aagaaaatga actaagtaat
    11461 atactgctgg tgacaaggat ggatcctaag atgaaaaggc taattattgt tgtggcatcc
    11521 aacggtcggt tactcctgat tacgctgtct cttatataaa ccctaccata ttagcggcag
    11581 agacagcgtt ttacttagcc ctgaagcgtg atttttttac attttaagaa caggatgacc
    11641 gctaatcgtc aactgagtgc cttcccgggt gttattcaga atggcatgtg cgcccaacgg
    11701 cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg gaatcgacag
    11761 gcgggaacgc aaaaacgcgt acactgactc caggttgtaa cctgcgtcat aatcattggg
    11821 cgtgctgccg ctaaagctac cgagaataat cgccttctga cgtggcaaaa ttcccgcatg
    11881 atagagctgc aacagcatac gttcgacccg gaaagggtgc tcgttaatat cttccagcac
    11941 cagaataccg ttctcaattt ttggcatcca cggtgtaccg atcagtgaaa tcagcatcgc
    12001 aagattgcct ccccacaacg tgccttcggc ccgacatgtc gggccttccc cttgccattc
    12061 aatggtgaag gtttcattgc gtaacgccag ccagaaatgg tgctcggtaa aggcgttcag
    12121 ttcatccgcg ccaaagtttg ctaccagcat ggggccgcta aaggtgatga cattgccatg
    12181 cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt ccgcaaatga gcaacgggtc
    12241 atgttgttgg cgagccacca gtgcctgcca gtcaatatct gccagtaaac gactggcacc
    12301 gtaaccgccg cgtacagcca gcacgatggt gttgggggta gtcagtctgg caagggaatt
    12361 gagatcttcc agacgctctg tttccgtacc cgcaaaacgc tcacaacgac gggcaataac
    12421 ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc tggataccac gcagcgcggc
    12481 gtgctgttta atgcagtaac ccgatggggc aattaagtga aacagagaca tggcaattcc
    12541 ttgctgacaa cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt gtcacctcaa
    12601 cggcgattcc aggctataag gatagaagaa gtgaaattga gatggtttgc ctttttgatt
    12661 gtgttattag cgggttgttc atcaaagcat gactatacga acccgctgtg gaacgcgaaa
    12721 gttccggtgc aacgtgcgat gcagtggatg ccaataagcc agaaagccgg tgcagcctgg
    12781 ggcgtcgatc cacaattgat cacggcgatt atcgctatcg aatcgggtgg taatccgaac
    12841 gcggtgagta aatcgaatgc cattggtttg atgcagttaa aagcttcaac ctccggacgt
    12901 gatgtttatc gccgtatggg ctggagtggt gagccgacga ccagcgagct gaaaaatcca
    12961 gagcgtaata tttcaatggg ggcggcttac ctgaatattc tggaaaccgg cccgctggca
    13021 ggcattgaag atccgaaggt actgcaatat gcgctggtgg tgtcatacgc taacggggca
    13081 ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa aatcaacgat
    13141 ttggatgctg acgagttcct cgaacacgta gcgcgaaatc accctgcgcc gcaggctccg
    13201 cgctatatct acaaacttga gcaggcactg gacgcgatgt aaatcagtcg cgcactttgt
    13261 ccgctttttc ccgggcttct cgctcgagag agaaaataat ccgctgtaat tgccgctcca
    13321 ccgtcgggct gacgttaaga aaacggaagc tcagacgggg agtggtgatg gtttcattct
    13381 tgccatcaat cactttgcgc tcgctgatgg agattaactg ggcgtcaaag tgaaaaacac
    13441 cccattgcct catgttgact tcaatctgag cgaagcgcat gccttcttgt aattcggcag
    13501 gctttgctgt ttccagtaat gcgcccatgc cgcctaacga caaatcatac aggcggaaac
    13561 gtaacgtact gttatccgcc agtttggtct ggcaaaaata aggcggatgg agtggggcgg
    13621 agatgcggaa atatcggcgt cgttgtacaa accataaggt gggaggcggt acggtgataa
    13681 atgccggaag ctgcaagtat tcactctgct gtagttgttc aacagtaaac tcgactttcg
    13741 caccctgagt ttcggcggta atggtaatgt gctgtgcctt tagcacggcg atgttgtctt
    13801 cggcttgact gccgaaatcc agcaccagtt tatccggggt tattgccagt attttgctga
    13861 tcagctgccc gccattccaa ctgagacgca aaggaattgc ggctttgtgc aaatcgcgta
    13921 acacgtccag gacggctaac ggattttgtt tcaggaactg ctcatggtaa tgactcacgc
    13981 ggaaaaactc ctgactgata aactgtcttt ggtttatcgg tcacagttaa caaaacttaa
    14041 tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc agccgacata cgcccagcat
    14101 cgatcccgcc cttgcctata cttagagcgt tgacgtaagc atttcttgcg tccgattcat
    14161 cgaaacgagg gcctgaacat gggaattatt gcctggatta tttttggcct gatagccggc
    14221 attatcgcca agctaatcat gccggggcgt gatggtggtg gatttttcct gacctgtatt
    14281 ctcgggatag tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg cattggcggc
    14341 tccatcagtg gttttaatct gcacagcttc ctggtggcgg tggtgggagc tattctcgtt
    14401 ctgggcatat tccgcctcct gcgaagagaa taagattttc ataaggcgga tagcgataca
    14461 gatgccgcta tccgctttca catcagaacg tatactcgac acctgcttta cgggtgaaaa
    14521 aaatcaatat agcacttcga tatccgataa agtcggttcc cggtaacaac aacagaatcc
    14581 ttttttaatt attgtttcgt tgtttttggt gtgattcaga aaataaatat tttagacagt
    14641 agccagtcag gcgttaaaaa tggggcggat agtgaggcgg ttttggctgc gcttattgaa
    14701 catttagaat tgatcaatcc ttctggcaga ttaagccaaa atacccgttc ggcaatgctt
    14761 cagttacgcg aagaggaatg gtctgaattc tttttttggt tgctaaattc tctggagtgt
    14821 ctggattatg tgattattaa tttaactcca gaaagcaaaa aaacgttgat gagtgaacac
    14881 cgtaacaata ttcaggtagc aattgatgct ttatatagcc aacgtcggcg taaatcgccg
    14941 ggtgatgaaa gtgaaacact tacccgtaga aatgacgcta tcttcggcaa ccacgtatgg
    15001 caaacatttg cgcaatactt cccgccagga cttgaaaaac cgtctgtgta gaaaaaacca
    15061 tagctatggt gttatctgcc gacaacgcca tccggagaag ccgaatgacg ttgtcggagt
    15121 aagcgctggt taaggtgtgg gttgtgcctc tttggttgag ggttgcgtcg ttgctgactt
    15181 aacggtcgga cgcgtcgccg gaacattgtc acacggttgc tctttcgggc agatcaaatc
    15241 cagcattttc agcgtcacgc cattggtcca gccaaagcca tcctgtaatg gatattcgcc
    15301 accgccgccc cccgttccgg tggtgctgac atcatatttt tccaccagct ttttctcccg
    15361 gtcataggtg tgctgaacat tggtcaggaa gtgccagcta atgtccatcg ccacctcttt
    15421 ttgcccgtag ttttgtaatc cttctgtcgc gacccactgt aacggtgccc agccatttgg
    15481 cgcatcccat tgttgcccac ttttcaccga cgtggtgttc aggccgccgg gttgcagcag
    15541 atgtgttttc gtcgccgtcg ccattttgtt ggcgcgatct ttcgctgccg cattgacgta
    15601 cagcgggaac agggcggccg cggttaactg attgcgcact ttatgacttt tcaggtcgta
    15661 atcggcatac cagccttgtt gatcgttcca caggtatttt tcgatccctt tttgacgggc
    15721 atttgccagc gtttcgtact ggtttgccat cgcgttatct ccggcagctt tgctggcgcg
    15781 ggcgaggatt ttttccattt taaacatcag gctgttcaga tcgaccggta cgatgctggt
    15841 ggtgcgtaag gtatttaact gctgcgggtt gtccatccag cgcgagctga aatcccagcc
    15901 agacgcagcg gcagagcgca ggtcgcggta aatttcagtg gcaggtcgat tcggattgct
    15961 tttggcggtg gcaatatctt ccacccatga ctctggtcgt ggcgtatcgc gatcgtccca
    16021 gtagcggttg agaagggtac catcctgaag tttgacaacg cgtttttcct gttgtccggc
    16081 ttgcaggttt tcaacaccgt ccatccagta agcatattct ttttgcattt gcggcaggta
    16141 ttgcttcaac gcggcatcgc cttcatgctg cgccagtaac tctaccatca gggcaaagaa
    16201 gggcggttgc gagcggctta aatagtaact gcggttgccg ttgggaatat gaccgtaagt
    16261 gtctatttca tgagcaaaat tggccaccat atccgcgact ttatcccagt gaccgctttc
    16321 ggcaagtcct aacatggtga agtaactgtc ccagtaatat acctcgcgaa aacgtccgcc
    16381 cggcacgaca taaggttccg gcagcggtaa cagagaatcc catttttcgg tgttttcggt
    16441 agaacgcgtt aataccggcc aaagtccgtc aatatgttcg cgcagtgact gtccctctgg
    16501 cggaacatat ttctcgccct ctttcggcag ggtgaaattg acgttaacga aatggcgcag
    16561 atcaaatccg ctctggtttt gctgcatccg ataatcagca aggatcatca gcggatcgct
    16621 gttcggcacg gcatcggcaa aggttttttg gtccggaaaa agtttggcgt tttgcacatc
    16681 attaaacagc ggccctaata aaatatcagg cggctgtggt gttaccggtg tttcttctgc
    16741 ctgcaccgat agcgcagcga aacacaaaaa gatgcaggct ggaattaacg ccattttttg
    16801 cgggcgagaa ggtgcggggg atttcatcaa tcattctcct ttggcgaaac cgaataaaac
    16861 ggtattcaag tatcagaaaa ccttagttca ggatcgcact agctgcatga tcgaaatctc
    16921 attctgcgaa ctgtcaggca ttttagaaaa gggaattaaa gggataaata acggcgaaat
    16981 agagtgatca aataacgtct gtttattagc cagccagcgg cgttgggttc gtgattagcg
    17041 aagaaaaatc atcataaatt ggtgaaaaat ataacaggta tttaaaaatc attagtgctg
    17101 ggtaaattgc cggatgacat cagaacgatg ccatccgaac ggtggcttaa ccctgacggt
    17161 tgaaacgttg cgttttaacg tccagcgtta gcgtttcttc tggttgtatc gcatacagtt
    17221 tctcaccctg ctggcaaatc cagccaatcc ctagttcacg ggcgattagg gcgctgtggg
    17281 ataccggact tccggcgcta aggcagatac cttttacaac cgccggatcc agttgcagta
    17341 ctgtggaagg ataaatgttc tccgccagta gaatagttgg cgagttaaac tgcgggagtt
    17401 cttctttcgt ttgggtcagg tggaccaggg tgcgatgcag aagatcgtcc acatcaatat
    17461 agcgaacttg tagatattca tcatccagtt gctggtattg ctggctaagt tctttaagaa
    17521 cttgctgcca ggcatattct gccgtgcaat gttcatgctg aaggagttcg cttgccgccg
    17581 ccagcagttc cggatcatct aacagtgtat ggtgaccaga aaagattgcg gcaatatcgt
    17641 caagcccgct ggcttctgct ttcgctgtta acgtcatcag atctaataac gtgaagtcaa
    17701 tagcctggcg taatcgttct tgttcttctt ccacggtcag ggttgatttt gcctgtaccg
    17761 tacataaaac tggttgataa taaaaggctt tacccgaaac aggcggaacg ggacgcagag
    17821 taggtggagc gacttcctcc gtttcaccaa agttatcttc agccagctga cggaaagcga
    17881 tcagtgcctc ttcagcttct ggccctttcg caatcaggcg cagcgtatcg ttatagcgaa
    17941 cttgtagtaa cgcaatctgg ttaatactct ctggtgtgac gcatttgccg tttttttcca
    18001 gcaacatatc ggcattaaat gtcgataagg tataaaccag ccgggaggcc ggacgtacat
    18061 gcaggccgtt acggtttttt atgaccaccg ccagagaacg ggcttcttca tcgtacgcag
    18121 gacatgtgtc agagatttca gtgtcggagg acggtaaacc cagttgttca cgtttggctt
    18181 ccagcgcatg catagcgtca aagataactt tgtcgatatc cgctcccgag gccgcgctga
    18241 ccgttgctgc cagtgtacct tcgaccaacg gcgcagcaca caaacgtact tttgcggcga
    18301 tctcgggagc cagcaattcc agcgcagttt cagcactcaa taatgcgcta cccatatcca
    18361 tcatgaccag cacatggtcg gcatcagcaa cagattcgat ggcctccatc actttgacgg
    18421 catcggtacc aatgggattt tgtggatcgt caattcccgc ggcaatggcg attttacaac
    18481 tatcactcat taacatctga cgggctaatt caccgacacc ttctcccagt cggctgctat
    18541 gtgaaactat gaccaggttt accatcacca attccttact cttttgcggc taacgccaac
    18601 atttgcatca taaacatcac cgaggtcgcg ccgggatcct ggtgaccaat actgcgttca
    18661 ccgagataac tggcgcggcc tttgcgggct tgcatcgtaa tcgtactttg tgcagcggat
    18721 tcggcgatgc tactggcagc ttcgagcgcc gccggaacag agagattttg ctcgctggac
    18781 tgacgtaacg attccaccac cggcacccac acatcacaca tggttttatc gccaggttcg
    18841 gctttcccgc gactgattac gccgtccgcg ccatcgcgga acatctgata aagctcttcc
    18901 agcgtcaggc tttgccgtgc ctgggtcgcc tgtgcggcgc ggataaagaa ggtaccgaac
    18961 agcggaccac tggcaccacc gacgctggaa agcagcgtca taccggtatt cttgagaatg
    19021 aaaccgatat ctttatctgc gatagcaggg agtttttcca ctactttgct aaagcctcgg
    19081 ttcatattta gcccgtggtc agcgtcgcca atttcgcgat ccagtccggt aagatactcg
    19141 ctctcggtgc tgaaaatatc gccacaacga gtgagccagt taacaatttg agttctgctc
    19201 agtgacattg ctttctcctt atttacccca gttaagggcc ggggtgtgga ccggggcgtc
    19261 ccagagtgcc agcgtttcgt catcaacttt cagtaaggtg attgagaaac cggtcatatc
    19321 cagtgaggtg cagtacgcgc caattaaatt acgttcgata gtcaatcccg cttgctggca
    19381 acgtgtggtc aggcggttat agacgccgta cagctcagaa agcggagttg cgccaagatt
    19441 gttaaccagc gcaatcaccc gatcgccaga ctggagcggt tgtttggttt gttgttcttc
    19501 ctgccaactg ccttgttgat aatcccagaa acgcaaagtg cgatggtatg agccattttc
    19561 cagcagggtg tcgaacattt catcgacggt ttgatcaagg gaagagaagg agcggcggtc
    19621 aatacccggc tcaccatgaa tgccgacgcc aaactccatc tcattatccg ccagggtaaa
    19681 agaaggtttg cccgcggcag gaacggtaca ggcaccgaga gcgataccta ttgagtggcc
    19741 ttgattattc aacttacgcc ccagttccgc acaggcgtcc agtgagtcgc cacgctcggc
    19801 cgctgcgcct acgagttttt caattaatac ggtgttggca acgccgcgtc gcccggcggt
    19861 ataaagactg tcttttactg caacgtcgtc atcaatgacc acagtggtca cgtttacgcc
    19921 gctatcgtgc agtaactcgg ttgctgtttc gaagttaaga atatcgccgg tgtaattttt
    19981 gataatcaac agtacacctt cgccgccatc aacttgcatg gcgcattcaa agattttatc
    20041 gggcgtcggt gaggtgaaaa tttcgcccgg acaggccccc gaaagcatcc cctgaccgat
    20101 ataaccacag tgcatcggct cgtgtccgct gccgccaccc gacagcaggg cgacttttcc
    20161 tgtaacgggg gcatcagctc gggtgacata caccggatcc tgatgcagtg tcagcgatgg
    20221 atgtgctttc gccagtcctg ccagttgttc gtccagtacg tcttgcacat cattgatcaa
    20281 ttttttcatt attttgctcc agcaattacg gtagggcatg gatgatgttc aacgacacgg
    20341 cgaccactga ctgccgatga atccattgtg catcaggcaa gggaaaagaa aattagccca
    20401 aattatgttt catagtgaaa catatgcttt aatgaatgtt ccatattgaa acttttacgt
    20461 gtattaatac ttaaaattgc gagccggaac accttttgtc ataagggatg cgggatatga
    20521 gtggcgcttt taacaacgat ggtcggggca tatctccctt aattgcaacc tcctgggagc
    20581 gatgcaataa gctgatgaaa cgggagacat ggaacgtacc acatcaggcc cagggcgtga
    20641 catttgcttc tatttatcgg cgtaagaaag cgatgctgac gctcgggcag gctgcgctgg
    20701 aagatgcctg ggaatatatg gcaccgcgag agtgtgcgct gtttatcctc gatgaaaccg
    20761 cctgcattct cagccgtaat ggcgatccgc aaaccttgca gcagctaagt gcactgggat
    20821 tcaatgacgg cacgtattgc gccgagggaa ttattggtac ttgtgcgcta tcgttagcgg
    20881 ctatctctgg tcaggccgtg aaaacgatgg ccgatcaaca tttcaaacag gcactctgga
    20941 actgggcctt ttgtgcaacg ccgttgtttg acagcaaggg ccgattgacg ggaacaatag
    21001 cgctggcgtg tccggttgag caaactaccg cagctgattt gccgttgacg ttggcaatcg
    21061 cccgcgaggt cggaaattta ctgctgacgg acagtttgct cgctgaaact aaccgtcatt
    21121 taaatcaact taatgccctg ttagaaagta tggatgatgg cgtgattagc tgggacgagc
    21181 agggtaattt gcaatttatt aatgcccagg cggcgcgggt cttgcgcctt gacgcgacgg
    21241 caagtcaggg acgggcaatc actgaactct taacgttacc cgccgtattg caacaagcaa
    21301 taaaacaggc acatccgctc aaacacgtag aagcaacctt tgaaagccag caccagttta
    21361 ttgatgcggt gataaccctt aaaccgataa tagaaacgca gggaaccagc tttattttgt
    21421 tgctccatcc tgtggaacag atgcggcagt tgatgaccag tcaattagga aaagtcagcc
    21481 ataccttcgc tcatatgcca caggacgatc cgcaaacccg ccgcttgatt cattttggtc
    21541 gccaggcggc gcgcagtagc tttcctgtcc tgctttgtgg agaagagggc gtgggcaagg
    21601 cactgctaag tcaggcaatt cataatgaaa gcgagcgtgc tgcaggtcct tatatcgccg
    21661 tcaattgtga gttatatggt gatgctgcgc tggcggaaga atttattggt ggcgatcgca
    21721 cggacaatga aaatggccgt ctgagtcggc tggaactggc acacggcggc acgctgtttc
    21781 ttgaaaagat tgaatatctg gcggtggagt tacagtctgc tttgcttcag gttatcaagc
    21841 agggggttat cacgcgactg gatgcgcggc gtttaatacc aattgatgtc aaagtgattg
    21901 caacaacgac cgcggacctc gcaatgctgg tggaacaaaa tcgttttagt cgccagctgt
    21961 attacgcgct gcatgcattt gaaattacca tcccgcctct gcgtatgcgg cgtggcagca
    22021 ttccggcgct ggtgaataac aaattacgca gtcttgaaaa acgcttctct acgcggctga
    22081 aaattgatga cgatgccctc gctcgcctgg tttcttgtgc atggccaggc aacgattttg
    22141 aactttacag cgtcatcgag aatcttgctc tgagtagtga taacgggcgc attcgcgtca
    22201 gtgatttgcc ggaacatctg tttaccgagc aggcgacaga tgatgtcagc gccacccgcc
    22261 tttccaccag tctgtcattt gcggaagttg aaaaagaggc aattattaac gcagcccagg
    22321 tcacaggcgg tcgcattcag gaaatgtcgg ctttacttgg gatcggccgc actacgctgt
    22381 ggcggaaaat gaagcaacat ggcattgatg cagggcagtt taagcgccgg gtatgaaaga
    22441 cagaaacgat ttctgataca tcagagtgat ctgtatttca ttccggcgca cgctaacaat
    22501 tttcagcatc gtttaagggc ttgtctatcc cgcacttaaa agctgaagcg atatcctccg
    22561 ttgacttgct tctgatcaaa gaggttaccc tgcgtgtaat ccgcttcgag atagaatgtg
    22621 tgctgtttgt tatactgtgc actgactccc acgccgttat tccagccatt acctttgaaa
    22681 ctgtacttct cccgggagtt gttcaacaga tattcggtat cccctgaaaa ctcgcggata
    22741 gcgccagtct tcacatagac attcagctgg ctgttgcctg cggtgatgtc ataccccagt
    22801 atcatgctgg cacgccccag cagcgattcg tagtgattca gatgtatatt gaggccatta
    22861 ctcgccttca tagtcatctc attctggtgg ctgtatgtaa gctgggtttg cggctctata
    22921 tagaacccat aaccagtagg ggacaggttg aacctctgcc cggcttccag ggagatgctc
    22981 attccattcg cagtgccgtt ggcgttaacg ccgttgttct gactgtccag tacgtggaaa
    23041 ctatttttct ggcgcgatgc ttttataacg agatcgctgt aaaaaccgtt ttgtgccatg
    23101 taactggcgt acattcccat gtagtctgaa cgtgcggtac cgtcgcctcc gctatagtcc
    23161 ggcgatgcat gtgttgagcc aatatacaga ccgacataca acggcattac atcagagaga
    23221 cgtttatccc caccaaactg gataccgctg taacccatgt caaagccgct cagtttgcca
    23281 ctggcaaagg agtccaggct tcccccataa ctgcgcaacc agatattacc gtctttactc
    23341 tgatttcgca ggtcacccat ccgttgcatc agcgtacggt tttcaacata gttcaataag
    23401 taaccgacat tgagataatt gccgccagca tctgcggtcg tggtgggttt aggcgtggga
    23461 gcgggttcag gcgtaggatc ggggttgact atgggaggct gagcgggagc tggtgtgggt
    23521 tcaggattag gagtcggttc cggaactgtc ccggaagcgt aaagctccca gttagtgcca
    23581 tttttacgca catcgtacag atatcccccc aactcaacct gagaagacgc gctgaacgag
    23641 gccgcgccgt cagtggtttt taccactgtc agaacttcat ttcccgttgt ggcctcgctg
    23701 ccctggttgc ggatagccaa tacgtgatta ccagcactac tcccgctgat attcaataaa
    23761 tcccctttat tattaacgcc attaccctcg ccaacaacat cagcacgcat aataaaggta
    23821 ctgttaccgc tcaggttctc tacgtttaat gtggcaaatg tgccggcagt tgacccgtgg
    23881 ctggcaaaat cgacagttga atggctcagc gccagcgtgt cgagattaga attacttgtt
    23941 acgttccaga cgctgttatt cattgcaacg tccagttttc cgccattgac attatcgctg
    24001 agggaggaac ctgtccaaac cgacccaggg tgcatatcca gattgattag cccacctttg
    24061 gaaagaacgc taccgttgat aagcatacga cccgaggcgt tgatgcggct ggcggcataa
    24121 ccatcgtcat gttgcgttgc gatggccatc tggtcgggtg tactcatatc aatgaccaga
    24181 tcgctcgtga ggtcgatctg gctgttggtc atggcataaa ttcccctggc tcctgccgcg
    24241 ccggtgatag ccaaactgtc tccggttatt ctaccgccgc tgagcgccca caaacccagc
    24301 gccagactgc cattacgatc aactgtaata tcggtatttt gcatgttgac aacagccgtt
    24361 gccgtctggg ccgaggcacc ataagaaccg ccggaaaaga tgctgtttcg ttgcgctgcc
    24421 gtgccagtaa aattgattat cgcgtctgaa ccactggtga cgaggccacc gccctgcgcg
    24481 gaagaaatat ggctatctgc accgatagtg gttgtaccac cacgaacttc gacgccattg
    24541 gccgcggctc cagttacatc aacagtgagt gcattcgcgc taacctggcc aaagctccag
    24601 aggccgtgag cattatcgcc attggtttta atggtactgt ttgttccgag atcgacaaca
    24661 gagtttttct gtacgtttat ccccatggcg ctgtagccct gaacatcgat tgtcaggtct
    24721 gtagccgtaa aacgcgcagc accattggcg ttattgccgt tgagaccacc gatataaaca
    24781 cctgtacttc catcggtcgt gattttactt ccgcttccaa gatcgacact ggtgccatag
    24841 tcattgatgg ttaggcctat accgttcgag ttttcaatgg tgaattgagt ggctgttagt
    24901 gttgagctat gcccaataat gatgccgtca tcgttactct taatggtgct gcctgtgcct
    24961 aagtcagcat gggtatagtc accaattaag ttaatgccga tggcagaggt ttgaccaaca
    25021 acatctactg tgagtcggtt agctgtcagg gagctgtttt tgccctgaat aatgattcct
    25081 tttgcatatc cagaggcgtc gttgacattg acggtgacat cattacccag gttgatattg
    25141 ccaggcgtgt ttcctgctgg ggtcattaca ccatagaggt ttcctgatgg atcggcggta
    25201 tctcctgtaa ttctgtcacc atcattaata gtaatattgg tgccatattg acttcctgta
    25261 tcagcagcta tcagcagggg ggaaaagata attgcattca aaccaatagc gccaaatttt
    25321 atcagttgaa ttgagggcga acggattttt aattcagcga gacgatctgc tttggtattc
    25381 ccattgtgtt gtttgatgcc cataccactc ctgtatagta cccagactat gggtctatgg
    25441 attttgtcat tttgtttaaa tcaatgaata atatcctctt atcatcatga tatttgtatg
    25501 gtgcaatcat gatgagcttc tcagagaata attctctatt accggg
//
LOCUS       DAZFZC010000076        25236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFZC010000076 DAZFZC010000000
VERSION     DAZFZC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.076"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..380)
                     /locus_tag="ACHKXP_003444"
     CDS             complement(<1..380)
                     /locus_tag="ACHKXP_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012667719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFO7648388.1"
                     /translation="MMILVTGGAGYVGSHTVLALLNQGINVTVIDNLSNSSIEAIRRI
                     EKISNKKVNFVHGDINNRAIIDRVFGDHKIKAVMHFAALKSVSESVKKPLNYYQNNVS
                     GTITLLEAMQKAGVNNFIFSSSATV"
     gene            complement(706..1599)
                     /gene="galF"
                     /locus_tag="ACHKXP_003445"
     CDS             complement(706..1599)
                     /gene="galF"
                     /locus_tag="ACHKXP_003445"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO7648389.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(1774..3168)
                     /gene="wcaM"
                     /locus_tag="ACHKXP_003446"
     CDS             complement(1774..3168)
                     /gene="wcaM"
                     /locus_tag="ACHKXP_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO7648390.1"
                     /translation="MPFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELYNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            complement(3179..4399)
                     /gene="wcaL"
                     /locus_tag="ACHKXP_003447"
     CDS             complement(3179..4399)
                     /gene="wcaL"
                     /locus_tag="ACHKXP_003447"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO7648391.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLNTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(4396..5676)
                     /gene="wcaK"
                     /locus_tag="ACHKXP_003448"
     CDS             complement(4396..5676)
                     /gene="wcaK"
                     /locus_tag="ACHKXP_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO7648392.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSSERQTGMLMVQSVLERIGEAK"
     gene            complement(6048..7526)
                     /gene="wzxC"
                     /locus_tag="ACHKXP_003449"
     CDS             complement(6048..7526)
                     /gene="wzxC"
                     /locus_tag="ACHKXP_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO7648393.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMIAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLVSVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYVLGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(7528..8922)
                     /gene="wcaJ"
                     /locus_tag="ACHKXP_003450"
     CDS             complement(7528..8922)
                     /gene="wcaJ"
                     /locus_tag="ACHKXP_003450"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO7648394.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(8977..10347)
                     /gene="cpsG"
                     /locus_tag="ACHKXP_003451"
                     /gene_synonym="manB"
     CDS             complement(8977..10347)
                     /gene="cpsG"
                     /locus_tag="ACHKXP_003451"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO7648395.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKTLGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELLCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(10540..11976)
                     /gene="cpsB"
                     /locus_tag="ACHKXP_003452"
     CDS             complement(10540..11976)
                     /gene="cpsB"
                     /locus_tag="ACHKXP_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO7648396.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLKGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAIPYAEAGKLVTFGIVPDLPEIGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKTMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(11979..13202)
                     /gene="wcaI"
                     /locus_tag="ACHKXP_003453"
     CDS             complement(11979..13202)
                     /gene="wcaI"
                     /locus_tag="ACHKXP_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO7648397.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADVDV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(13199..13678)
                     /gene="gmm"
                     /locus_tag="ACHKXP_003454"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(13199..13678)
                     /gene="gmm"
                     /locus_tag="ACHKXP_003454"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO7648398.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDEMLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDVLLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(13681..14646)
                     /gene="fcl"
                     /locus_tag="ACHKXP_003455"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(13681..14646)
                     /gene="fcl"
                     /locus_tag="ACHKXP_003455"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO7648399.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(14649..15770)
                     /gene="gmd"
                     /locus_tag="ACHKXP_003456"
     CDS             complement(14649..15770)
                     /gene="gmd"
                     /locus_tag="ACHKXP_003456"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO7648400.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(15796..16344)
                     /gene="wcaF"
                     /locus_tag="ACHKXP_003457"
     CDS             complement(15796..16344)
                     /gene="wcaF"
                     /locus_tag="ACHKXP_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO7648401.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(16360..17106)
                     /gene="wcaE"
                     /locus_tag="ACHKXP_003458"
     CDS             complement(16360..17106)
                     /gene="wcaE"
                     /locus_tag="ACHKXP_003458"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO7648402.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSN
                     DGTREYLENLNGIFNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(17117..18334)
                     /gene="wcaD"
                     /locus_tag="ACHKXP_003459"
     CDS             complement(17117..18334)
                     /gene="wcaD"
                     /locus_tag="ACHKXP_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO7648403.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(18309..19526)
                     /gene="wcaC"
                     /locus_tag="ACHKXP_003460"
     CDS             complement(18309..19526)
                     /gene="wcaC"
                     /locus_tag="ACHKXP_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO7648404.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(19523..20011)
                     /gene="wcaB"
                     /locus_tag="ACHKXP_003461"
     CDS             complement(19523..20011)
                     /gene="wcaB"
                     /locus_tag="ACHKXP_003461"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO7648405.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(20014..20853)
                     /gene="wcaA"
                     /locus_tag="ACHKXP_003462"
     CDS             complement(20014..20853)
                     /gene="wcaA"
                     /locus_tag="ACHKXP_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO7648406.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(21031..23193)
                     /gene="wzc"
                     /locus_tag="ACHKXP_003463"
     CDS             complement(21031..23193)
                     /gene="wzc"
                     /locus_tag="ACHKXP_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO7648407.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(23196..23639)
                     /gene="wzb"
                     /locus_tag="ACHKXP_003464"
     CDS             complement(23196..23639)
                     /gene="wzb"
                     /locus_tag="ACHKXP_003464"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO7648408.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(23645..24784)
                     /gene="wza"
                     /locus_tag="ACHKXP_003465"
     CDS             complement(23645..24784)
                     /gene="wza"
                     /locus_tag="ACHKXP_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO7648409.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         5943 a         6827 c         6244 g         6222 t
ORIGIN      
        1 actgttgcag atgaactaaa aataaaatta tttacacctg ctttttgcat agcttccaat
       61 agtgttatcg ttccagaaac attattctga taataattta agggtttttt gacagactca
      121 cttaccgatt tcagcgctgc aaagtgcatt acggctttta ttttatgatc accaaaaact
      181 ctatcgatga tagcacgatt attgatatcg ccgtgtacaa aatttacttt tttgttgctt
      241 attttttcaa tacgtcgaat agcttcaatt gacgagttac taagattgtc aataacggtc
      301 acattaatcc cctgatttag caaagctaaa acagtatgcg atccaacata gccagcgcca
      361 ccagtcacta atatcattat cagtacctgc taataagaaa taaaaacaca cgctaccgcc
      421 cctggcttta cagctaccag agcactgcat gcatgcctac gatgtgacga gcgttaccca
      481 ctcgcgctta ccccgaaaaa ttcaaacgct aattgtctta ccaatccgct ctggaaacaa
      541 ggaaaatcct ggaaaacttt gactaaaatc ctattgctaa ctcgttgtta ttctgattgt
      601 ttatataaaa taacggcagg aatattcgca acaaattact ttcgccacga atcttcactg
      661 ccgttataat tttcttatca accgttacat ccggtcagat tttcattatt cgcttagcag
      721 cttctcaatc cctttacgga acttcgcccc ttctttgagg ttgcgcagcc catacttcac
      781 gaacgcttgc atatagccca tttttttacc gcagtcgtag ctgtcacctg tcatcagcat
      841 ggcatcaacg gactgttttt ttgccagttc ggcaatggca tcagtcagct gaatacgccc
      901 ccatgcacca ggctgagtgc gttcaagttc cggccaaata tcggcagaaa gcacatagcg
      961 accaacggcc atgatgtctg agtccagtgt ctgcggctga tccggttttt cgataaattc
     1021 gacgatgcgg ctgactttac cttcgcggtc cagcggctct ttggtctgga tgacagagta
     1081 ttcagagagg tcacccggca tacgttttgc cagcacctgg ctgcgtcctg tttcattgaa
     1141 gcgcgcaatc atggcagcaa ggttgtagcg cagcgggtcg gcgctggcgt cgtcgatcac
     1201 aacgtctggc agcaccacga caaatggatt gtcaccaatg gcaggtcgtg cacataaaat
     1261 ggagtggccc aaacctaaag gttcgccctg acgcacgttc ataatggtca cgcccggtgg
     1321 acagatggac tgcacttccg caagcagttg acgcttcacg cgctgctcaa ggagagattc
     1381 taattcataa gaggtgtcga agtggttttc gaccgcgttc ttggacgcgt gagttaccag
     1441 gaggatttct ttgatccctg cagccacaat ctcgtcaaca atgtactgaa tcattggctt
     1501 gtcgacgatc ggtagcatct ctttgggaat cgccttagtg gcaggcaaca tatgcatccc
     1561 aagacccgct acaggaataa ctgcttttaa attcgtcatt atttcatcca cctgtaaaat
     1621 ggttgctgaa ttatagcttg ttcgattttt ttcgccagca tcaattaccc tgaattgatt
     1681 actgaattac ttgtgatgtt acgccgcttc gttgtggatt gcagtagcat tgttcctaag
     1741 tatggctcca tttttccagg aatggtcgca aatctactct ctccgttccg gcaatctaaa
     1801 gttaatcttt tccacattaa caatatggtg attaaccctg tcgatatcga cggagctttg
     1861 tcctttctca ttcaccgcat gaacatttgc aagagacagc agtgtttctt ttttcgccat
     1921 aaagacgccg cgaacgtctt tgcgcatgtc gaagttcatg ctcaatgcgg gtccaactga
     1981 ggattcctgc atcacattga tattacgcat aaaaaggtgt tgcggtttat tgtatagctc
     2041 aagcgacgca cgcttcatct caatgttagt cagcgccaca aaggagacgg cattcccggc
     2101 ggagatttgg ataccgcgca atttataagc aaggtgggta ttatccagtt gaatattatt
     2161 cactcggaaa ttttgcggta tcgagagata tttgccttta attaccccat agccgattaa
     2221 catcccggcg ctattaatca tttcaatatt atcaatcacg aaattgtcac aaccgtaaat
     2281 agcaactgtc gcgttatcaa tgcctgcttt cttactgaaa tccggcgtga tattgcgggc
     2341 tttgatatta cggataacaa aatgtttgcc attttcaaca tggatcaact gccgacaatc
     2401 cgatcccgtg atattcgcca cgacaaagtt tttcactgcc tgatcttccg ggtaattatt
     2461 atcgtaagtg cttcccgcaa ggcctatgcc aatgccccag ttgattttgc cgttggtaca
     2521 gttgatgcgc tcgatgacat ggtcggagat caaaatatca ctgtcgttaa tcgccacgtt
     2581 ccattcaata gcgtccccct gtaaatcact aaatttacag ttggtgatgt tggcaccaat
     2641 tatctggtta tgaaatccct ggcgtaagat ggcgtaatta gcgtggctga cagtgaggtt
     2701 atcgatggtc aggttgcgca tgacccgttt gtttttgccg ccgatataaa tctgcgttac
     2761 cgggccaaag ccgctcatcg ccagcccttt gatggtgcag tcagaaccac gcacatccag
     2821 ggtgatgtta tgcatactgc cgccctcctc ccctgtcacc tggctgccgt cctgtaagac
     2881 aaatcgccct ctgccgttgc cgcgcaggct tccaaggatg tgtaacgttt ttccaggagg
     2941 aatgaagatg ccggtgttga tattgtcaca aacgaatcca gcaggcacga cgaccgtttg
     3001 cccttcgctg aaggcttgtt taaatgaggc gatccagtcg tgagggttgt agtcgttaat
     3061 gttaacgctt tgtcgggcgg gaagtgcgcg agcgaaaggg gtatggagga aggcaagcgc
     3121 cgagcttgcc gtcaggaagg tgcgtcggga gattttttta aatggcatac gttctcctct
     3181 ataaagcctg caacaagctg gcgagttctc ggttaatcac ctgctggtta aaatcgtgtt
     3241 cgactttttc gcgcgcacgt ttgacaaccg gagccagttc gtcggtgtta agttggctaa
     3301 acgccgccag tcgttgcgcc agtgcgcgag catcgttctc aggcaccagc cagccggatt
     3361 tgtcggcctc caccaattcc ggtattccgc tatgcagagt agaaaccacc ggaatgccga
     3421 ccgccatcgc ttccattagc gccactggaa tgccttccat atcgccatcc gcgcccgtta
     3481 ccgatggcaa caggaagaca tccgcgtcgt cgagcatcgc tttcacttcg tggctcggtt
     3541 taaagcccgg catctctacc acatcttcca gttgatattg ttcgatgagg gtacgcaggc
     3601 gtcgttccca cgggccaatg ccgaggatgc gatagcgaaa tgccatgccc tgctctttca
     3661 actgacggca ggcttcgatc gccacatgca ggcctttttt ttcggttaag cgtgcgacag
     3721 atatgatttc cagcggcgtt gcgggcgctt tcaccggacg cgggctaaaa cgcgtcatgt
     3781 ccacgcccat gcgcgatacg gcaatttttt ccctcgggca gcccattttt tgcagccttc
     3841 cggcccacag atcgcttatc ggtaacatca ggtcgccacg gcgaaacagt tgttgatatt
     3901 cgggagtgta gtggttgagc acttcccgac tggagatatc aataccgtgg aagatggtgg
     3961 caattttgcc gcgaatgaca cccagttcgc gtagttttgc tgcggttacc cccgcagggc
     4021 caaaatgagc gatgaacaca tcagcatgaa acggtgttgc aacctggccg caaatggcag
     4081 acaaaatcag gttccgcgac tctgcgccat agcgtttgag gttgagcgcc tgccaggtat
     4141 ttttacgatg aatgccgcgt aaggtctggc tggcgcggtg gcgcagcttc gccactttgc
     4201 ccgtaggttc gtcctgtaac cagcgggttc tggcggcaag gttgtatttc gtccatgccg
     4261 cgtgggtgtt ctgggtgtcg cctttttgca gcgcaacaat ctccacctca aatcccatat
     4321 caataaacgc ggtaatttga ttgaggacga aggtttccga cgacagcgga aatttcagta
     4381 aaaaaaagcc gaccttcatt tcgcctcccc gatgcgttca agcacagatt gcaccatcag
     4441 cattcctgtc tgacgctcac tactgacagc ttcgttaagt cgtgcgttca gcgccggaag
     4501 ttggcctaag gtatccgcaa ccatcgcttg caagctaccg tccagtaaat gacggatatc
     4561 aatcgccatc tctggcagcc ccagttgttg cataatcccg gcggatttgt gttcgtagtt
     4621 gatggcaatt gccggagtag caaaattcat cgagataatg gcagagtgca ggcgcgtacc
     4681 gacggtgagt tcacaggctc cgagaatttt gcccatttcc agatcgttga gttcatccat
     4741 cactacgtgg taacgggcag gatcgctgat gtgctggcgc aggttgagcg ccaccatgcg
     4801 gtcgtctttg ttatagctgt cgatacccgt acaggtggag agcgcaatca cctgataccc
     4861 ttcgtcgaga atgcgattga ccaccccggc aaaggctttt tcatacgctt gttgagtggt
     4921 gccgagacgt ttgtcgaacg gtgccagttc gcgcagggta atggccaccg ttttctgttg
     4981 tgcggcaacg tccagccagt gttgaacggc atagctggca gtgaagtctt cggtgtggtg
     5041 atcgaccagc cacgcggtat cgacgccatg ttccactttt gcggtagtga tattgctgcg
     5101 tttcatcaaa tcaaggctga ccgattcgcg caggatcagc gcgtcgcaat gaccaaagac
     5161 atagttcgcc agttggttaa attgctcatc ctggaaggga ccgacgctgt gaccaatcat
     5221 aaacagcggc tttttcgcca taaacgtgca aagtgcgtgt tcaaactgcg gcacaccata
     5281 gagatcgaca aaaaacgatc cgccgacctg aataatggcg tcgtaacctg acagcaagcg
     5341 cacgaagtcg gtgaatccct gggcgatggc gatattacgc agcttgccag tgtcggtgac
     5401 gcgtgagagc aatacctgat gctggtagcg gcgacggagg acttttttaa cgcgcccgac
     5461 aacgcccgcc gcgctgttgt gttgtttcat ttgcaggaac agcggatcgc ccattaccgg
     5521 gcggttgagc agccaggaag aactgaccgg atagcggctc atcacgtcca cttcggcgtg
     5581 tggattgagg atgttgatgg catcaagtaa gccacgcaag atggcgctgt cgccacgatt
     5641 gccgcaagtg tggttgccca gaataagtaa tttcattttt tcctcataaa tttgatgcca
     5701 ggtgaggctg cgtttgtttt gttcgttgcc gttttttgcc tgatgcgacg ctggcgcgtc
     5761 ttatcaggcc tacaagaccc gagcacagaa ccgtaggccg gataaggcgt tcacgccgca
     5821 tccggcattc agtgcctgat gcgacgctga cgcgtcttat caggcctaca agtcccaagc
     5881 acagaaccgt aggccggata aggcgttcac gccgcatccg gcattcagtg cctgatgcga
     5941 cgctgacgcg tcttatcagg cctacaggtc ccgagcacag aaccgtagga tggataaggc
     6001 gttttacgct gcacccggca accgttgtcg gaaccgaaaa caacaactca ccccgcccgt
     6061 aaaagcattt tcattttttc gctgcgacaa aactgacgct tcacttccac caccagcgga
     6121 tggcgcgaca gcacaatcat caccacaaac gccagcacgc ccgcggctat ttgcaccgcc
     6181 agcagcatcc ccagcgccag ttgccctttc agcacaatgc ccagcacata actgaccacc
     6241 agcgtcggca gcgagagata aaacggcagc cacaaactca ggatgtactg gcgataactg
     6301 gaaccaagaa ccggtttaat catcacgaag taactcagaa tggtgttgat aatctgcacc
     6361 agcaggaaac cgagcgtgac gccgattgcg cccgccatct gcccaccaat aacaatcgcc
     6421 ggaataaaca gaaaggtttt gaatacgttg aatttaaagc tgatgtcgac ccgcgctttc
     6481 gccatcagca gcgaaccaat cgggttaccc acggagcgca gcagacccac aatacacagc
     6541 aattgcagca ccggaataat gctgttccac ttttcgccga ataccagcgg tacgaagtta
     6601 ttcgacacca ccatcagccc cagcagcgct ggaaagttaa taatccccac caccgacagc
     6661 agcttgtaga agttgacgcg cagcttttcg gtgtcgtcct gaattttggc gaatgccgga
     6721 aacaacacac gggtgatgat cgggttcagc ttcatcggcg gcacaacggc cacgttgtac
     6781 gccaggttgt atcccccggc cacgcccgcg ccgagaatac gcgccagcac cagcgttgaa
     6841 aggttggtgt tgagatagtt gatgatgctg tccgccgtca gccaggcacc aaagcgtaag
     6901 ttcggtgcca ccgacaccag cgagaagtgc agaccggggc gataaatttt gcggccaaag
     6961 tagccaaaca gcagcgttct taccgcacta ttgaccagat aaccgaggat cgcgatcatc
     7021 gccagcggcc agaaatgggc gctaaccacc gtaaaagtaa agcccgccag caccgcgctg
     7081 gtttcgatca tgccgatttt gttgaactcg agctccttct gcatcaacgc gcggaactgt
     7141 tgcccgtggg ggatcaccac aaacgccagc gacaatgttt taatcaacgg tgccaggtcc
     7201 gggttattca gcacgtcacc aatgagatca ctcaacaaaa acaccgccac gcacaccaca
     7261 agccccagcc cgacgttcag ccagtacaac gtggtgagtt caaggtgact gatttctttt
     7321 cgctgaataa tcgagttagc gataccgaag tcagaaagcg tatctgccag cgcgataatc
     7381 accagcgaca cggtaagcag gccgaactgg tggttgtcga taatccgcgc cagcacggtc
     7441 atctgcacca gcccgaggcc gatgatgatc accgtggcaa tcgccgacca cttcgcgccg
     7501 ctgatggtct tttcacgtaa gctcatatca atatgccgct ttgttaacga agcccttgaa
     7561 taccgtcagg aaaacgattt tgatatcgaa ccagacgctc cattcgcgga tgtactcaag
     7621 gtcgaactcg acgcgttttt ccattttctc cagcgtgtcg gtttcgccgc gccagccatt
     7681 aatctgcgcc cagccggtaa tgcccggttt cactttgtgg cgcagcatgt agccttcaat
     7741 gagctggcga tactgttcgt tatgcgccac cgcgtgtgga cgtggcccga caatcgacat
     7801 ccccccggtc agcacattga taaactgcgg caattcgtcc agcgaggtgc ggcgcagaaa
     7861 gttccccact ttggtgacgc gcggatcgtt ctgcgtcgcc tgagtcacga ctttgtcgtt
     7921 ctccatcact ttcatcgaac ggaacttcca cactttgatc ggcttgccat ccatgccata
     7981 gcgagtctgg cggaaaataa ccggtcctgg tgaactgagt tttaccgcca gcgcaataca
     8041 gcacagcacc ggggagatca gcagcagaat aagcgtcgcc agcacaatat cttccgcacg
     8101 ttttagcagg cggttaaccc cggaaagcgg cgtatcgtac agcggcacca ccggaacgcc
     8161 gttcatctct tcgagacgtg aatggagaat gttgaaggta aagacgtcgg ggatcagcag
     8221 caccgaacag gtggtgtccg ccagttgatg gaccagtttt ttcactcgtg cgccgtcgca
     8281 catttgcatc gcgatataga cgttatgaat cttgcccgct ttcgcatcct cgaccagctg
     8341 ttgcagattg cccgcccagt cgttagaaac gccacccggt ttcgggtcgt ggtaaacgcc
     8401 caccacttca aaccctaacc acggctgatt acggaagctc tccatcagca tttgtccggc
     8461 ggctaaatct cccgctacgg cgaccatacg cttgttatag ccatgattac gcagccagcc
     8521 cgccccaatg cgaatacatg aacggcaaac caccagtccg atactggtca gcccatacca
     8581 cgccagccag attttcagtt gcgtgtcgaa atcattattg aacgccacca gtccggcgct
     8641 gaaaatcacg cttaaggtcc agttttgtag caacagggca aattctgtcg ctgcccgaac
     8701 accgcgccat gagcgataaa aatcggtgat gccgcccagc atctggaaca ccaccagcgt
     8761 aatcaacgcc accaacaggt gcatgtagag gaatgacagt ccgctcactt cgcaaaccag
     8821 ccatagtccg gcaaacatga tggtgatatc tgaaaagcgt tgcaccatag agattaacga
     8881 tgcattggtt ttcgctcgct cgcgcttttt tagatttgtc atcgttgttc ctgttattag
     8941 ccccttaccc gcagtggggt aagggaagat ccgacattac tcattcagca acgtcagcag
     9001 agttcgcgtt cgcgcttcca tcagcggcac atcaccgcgc gattccacat tcaaccgcac
     9061 caccggttcg gtgttggagg tgcgcaggtt aaagcgccag tcggcaaagg tcatgctgat
     9121 gccatcggta cgatccaccg ccagcgcctc acggctaaaa tgctgttcga cgcgattaat
     9181 cgcctcaacg ggttgcgcca gtttgctgtt gatctcaccg cttgccggaa acgccgccat
     9241 ccggtcgcgc accagttcgc ccagcgtttt tcctttcagg cacaacagtt cggcgaccag
     9301 cagccacggg atcatgccgc tgtcgcagta agcgaaatca cggaagtaat ggtgagcgct
     9361 catttcgcca ccgtagatgg cgtcttcctt gcgcatccgt tctttaataa aggcgtgtcc
     9421 ggttttcgac attaccgggg tgccgcctgc ggcagtcacc acatcaatag tgttccagga
     9481 gagacgcgga tcatggatga tcttcgcgcc gggatttttt tcgaggaacg cttctgccag
     9541 caggccgaca atgtagtagc cctcgataaa ctgccctttt tcgtcaaaca gaaaacagcg
     9601 gtcaaaatcg ccatcaaagg caatgcccat atccgcgccg tgtttgatga ccgcattgcg
     9661 ggtgtcgtcg cggcattcag gcagcagcgg gttaggaata ccgttgggga aattgccgtc
     9721 gggcgtgttg tgcactttga tcaattccac gggcgcgccg agggttttaa agcgggcttc
     9781 aatggcgtcc accaccggac ccgctgcgcc attcccggag ttaatcacca gcttgagcgg
     9841 cgtgaggttt ttgacattga tataaccgaa caggtgatca acgtaagcgt cacgcaggtt
     9901 gatttgctga tagcgaccgc gtttggtttc atcgacggga ggaaagtcgt tggcttctgc
     9961 cagacgctgg acgtcgcgca gtccggtatc accgctgatt gggcgagccc cttcgcgcac
    10021 cagtttcatg ccgttataat ccatcggatt atggctggcg gtaacttcaa tgccgccatc
    10081 cacgccgaga tggaacgtgg cgaaatagat ctcttcggtg ccggacatgc caatatccag
    10141 cacatcgacg cccgcatcct gtaaaccttt cgccagcgcc agttttaagg tttcgctggt
    10201 gagacggaca tcaccgccta acacaatggt tttcggtttg agaaattcgc cataggcgcg
    10261 accaatgcgc caggcgatat cttcattcag ttcttcgcct aatttcccgc gaatatcata
    10321 ggctttaaag caggttaatt ttttcatgtc gttacccttt ttcaggcaat agttggccct
    10381 gaccgaaacg gccaattttg tttttgtcgt tatttacatc tcggtttcgc attcattgcc
    10441 tgatgcgacg ctgccgcgtc ttatcaggcc tacaaatccg aaccgtaggt cggataaggc
    10501 gttcacgccg catccgacaa acagcgcctg atgcgacgtt tacacccgtc cgtagcgatc
    10561 cgcgaagcgc accacatcat cctcttcgag ataagagccg gagcgcactt caattaaatc
    10621 gagcggaatt ttccccgggt tttccaggca atgcgtcgcc cccagcggaa tataaatgga
    10681 ctcgttttca ccaagcagtt tgatatcacc gtcgatagtg acttttgccg ttcctgcgac
    10741 aaccacccag tgttccgcgc ggtgatgatg catctgtacc gacaagcctt cgcccggttt
    10801 cacggtgatg cgtttcacct ggtagcggtc gcccgcgtcg atagagtcat atttgcccca
    10861 cggacgatac acttcgcgat gcacccgatg ctcatggcga ccatcggctt tgatctgctc
    10921 gaccactttt ttcacatcct gcaccgcgtt acggtcggca atcagcactg catctttggt
    10981 ctgcactacc accaaatctt tcaccccgac ggtggtgacc aggccagatt cggcatacac
    11041 atagctgttt tcagttttgt gattaatcac atcgccgtgg caaacattgc cctcggcggt
    11101 gtgggcgctg atctcccata atgaagacca agaaccgaca tcgctccagc ccgcatccat
    11161 cggcaccaca acagcatctg ccgtacgttc catgaccgcg taatccaccg actcttccgg
    11221 gcaggcgaga aacgcttctt catccacgcg aataaaatcg agatccggat cgacggcgct
    11281 catcgttttt tcacaggcat cgagaatatc cgggcgatat tttttcagtt cttcgagata
    11341 gcgtccggcg cggaacagga acatgccgct gttccagtaa tattcgccac ttgccacata
    11401 ggcctgagcg gtttccagat tcggtttttc gacaaactgc gccacttcaa aggccaccgt
    11461 atcctgctca cccgcagaca cttcaccgcg acgaatatag ccataaccga tttcaggtag
    11521 atccggcaca atgccaaagg tcaccagctt gcccgcttcg gcatacggta tggcattacg
    11581 cacggcggca cggaacgcgt cttcatcggc aatcacatga tccgccgcca gcaccagcat
    11641 taacgggtcg ctctccggac tatgacgtgt tgccgccagc gccgccagcg caatggctgg
    11701 tgcagtgtta cgccctgccg gttcgagaat aatgttctcg gtgagtttgt tcagttggcg
    11761 cagctgttcc gcgacaataa agcggtgctg ctcattgcaa atcaccaccg ggctttcgca
    11821 ctctacgcct ttcaggcggc agatggtggt ttgcagcatg gtgagatcgc ctttcaggca
    11881 taaaaactgc ttggggtaaa gtacgcggga aagcggccat aagcggctac cggagccacc
    11941 tgccatcaca actggataga gtttcgactg cgccataatt atccccgaat atcatttata
    12001 aattgacgta acacgttctc tttatcgagc gtgcgttcgg catattcacg tgccaccgtg
    12061 ttgtgtttgg gcagcaggag cgcctgacga atccccgcca ccagcgcctc gaccgattcc
    12121 ggttcaacgc aaaccgcaat gcccggaaag gtttcgcaaa gctgtcccag ttctgtgtgg
    12181 gcttctgcag taatcaccgc gttaccgcct accgccagaa tattggtcag tttcgacggc
    12241 aatacggcat ctgccgcgcc gcgtttttgc accaccagat ggcaatcgcc catcttcagc
    12301 agtgcgggta aagcgtcata cgattgcagc ggaaaaaatt gcatgttgcg cagaccacgc
    12361 tgttgcgcca ttttttccag ccgcgctttg ccgccgccct gcccgacaat ggcaaaaatc
    12421 agcggttcat cacgcaggcg atcggcagct tcaataacgt tttccagccc ctgcttttca
    12481 ccaatattgc cggagtaaag aatgattttt ttgttatccg gcaggccaag ctggttacga
    12541 agggcatcaa catcgacatc tgcgacatgc tgaaaacggg caatttctga ccagttgggg
    12601 aagaagatga cgttttctgc cgccacgcct ttttcgatgg ctttattcat catcgaacgc
    12661 gaaatcgtgg agacgttatc gacgttatgc agtccgctac gttcgaacgc cgttgccagc
    12721 tgtgccactt tgccgccttt gccttttccg gcaaggccca gccccagcat ggcgtccact
    12781 tcgtaatcct gaatatgcag cacggtacgc gcgccagaga gtttcgccag caggcgcatt
    12841 cccggcgtgc aaaacagcgt tggcactacg ccgataatgc gatccggctt ccagcgacgt
    12901 tgcgccatca acgggaaaaa actgctgacg gcaaaactgc cgagatgcaa caggcgtttc
    12961 agggtgctcg gctgttttgg cacgtacagc gggcagcgcc acaccgtagc ggccccctct
    13021 tctcgtttgt accgccaggc ggaatagttc tcgcccacct gccactgcgg gtagtaaggc
    13081 ggtgcggtaa taacccgcac ctcatgacct tgcgccgcca gccattcaac catctctccg
    13141 gtgtatttgc cgatgccggt taactccggc gagtagttaa tgccgtagac cagtattttc
    13201 ataatccggg tactccggca cgcttctcag cgagaaaata ggcgcggctg ttagcgtgaa
    13261 catcgttact ggcgagcagc acgtccggcg tcagccagcg gtaatcgtca tgctgctcat
    13321 ccggcagtaa cagatcttct tcagcgactc tgaagcgaaa accgagcacc acatagtgag
    13381 tggtgaaatc cgtgccggag aagttatcgt cataaaagtg ctgccagaca ccgtaaaact
    13441 ggcctgctgt tatcggtaga cgcagcccca gttccgccat cgtcagccgc tcaaatgcgg
    13501 cttccagcat ttcgtctttc tgcacgcgcc ctcccggcac aaaccagtaa ccctgcgccg
    13561 ggcggttggt tcttttgcca agcagaaact cgccgcgact gttctcgaca ataaaatcga
    13621 gagagacaag cggagtggag cgcactaccg tggcaaagtc ttcctgacgt aaaaacatca
    13681 ttacccccga aagcggtctt gattctcaag gaaccactgg taagtgctgg caagccccgc
    13741 ttccagtgag atttcgtgat accagccaag ctgatgcagg cgcgtcacat ccagcagttt
    13801 gcgcggcgta ccatccggct tgctagcatc aaaaaccacc cgacctttgt aacccaccac
    13861 tttggcgatg gtttgcgcca gctcgcggat agtgcagtca acgcccgtgc cgacgttaat
    13921 gtgtgacagc atcggctggg tgttttccag ccagacttcg tgcgccagct ccatcacatg
    13981 aatgctcgcc gccgccatat catcgacgtg cagaaattca cgcatcggtg taccgctgcc
    14041 ccacaccacc acgtccggcg cattctgtgc cgtcgcctcg tggaagcgac gcagcaatgc
    14101 tgggatcaca tgcgagttac tcggatggaa gttgtcgtgt ggcccgtaca ggttggtcgg
    14161 catgaccgag cggtaatcgc gtccatactg gcggttgtaa gattcgcaca gtttgatccc
    14221 ggcgattttg gcaatggcgt aaggctcgtt ggtcggctcc agcgtaccct gcaataactc
    14281 gctttctgcc atcggctgtt ttgccagttt cgggtagata caggacgatc cgagaaacag
    14341 cagtttgttc acgtcgttct gatgcgcggc gtgaatgatg ttgctctcaa tcatcatgtt
    14401 ctggtagatg aaatccgccg gatacgtgtt gttggcaaca atgccgccca ctttcgccgc
    14461 cgccagatag acctggtcaa tgctttcgct ggcaaagaaa tcatgcacag cgcggctgtc
    14521 cagcaggttc agctcgtcgc gggtgcgtaa taccagttcc acatcaccgc gctgttcgag
    14581 ctgccgcctg atggcagaac cgaccatccc gcgatggcca gcgataaaaa tgcgttgttt
    14641 actcatgctt atgactccag cgcgatcgcc acgtcgtagc cgtgagattt cagcagagag
    14701 tgttttttcg ccgcttcgag gtcattagcc accatttcag acaccatctc tctgagggtg
    14761 atttccggtt tccagcccag tttttcgtgc gctttggtcg ggtcgccgag cagcgtttca
    14821 acttctgccg gacggaagta acgcgggtca acggcgataa tcacatcacc cggtttaacg
    14881 cccggcgcgt catgcccggt gacggaaacc acaatgccct tctcttcaac acccgtgcct
    14941 tcaaagcgca gtttgatgcc cagctgtgct gccgccattt ccacgaactg acgcacggag
    15001 tactgaacgc cggtcgcgat aacgaaatct tccggctgtt cctgctgcag catcatccac
    15061 tgcattttta cgtagtcttt ggcgtggccc cagtcacgca gggaatccat attgccgagg
    15121 tacaggcacg actccagccc ctgggcgatg ttggcgattg cgcgggtgat tttgcgggta
    15181 acgaaggttt cgccgcggcg cggggattca tggttgaaga gaattccgtt acaggcgtac
    15241 atgccgtagg attcacggta gttaacggtg atccagtagg cgtacagttt ggcgaccgca
    15301 tacggagatc gcgggtagaa cggcgtggtc tctttctgcg gaatttcctg caccagacca
    15361 tacagttcag aggtggaagc ctgatagaaa cgagttttct tttccagacc gaggaagcgg
    15421 atcgcctcca gcaggcgcag cgtacccatc gcgtcgacgt cagcggtata ttctggtgac
    15481 tcaaaagaga ccgctacgtg gctcattgcg cccaggttgt acacttcatc cggctgtact
    15541 tcacgcaaaa tgcgcgtcag gttagaggta tcactcaggt cgccataatg cagatggaat
    15601 ttcgggttgc aggtgtgcgg atcctgataa atgtgatcca cgcgctcggt gttgaatgac
    15661 gatgcgcgac gcttaatacc atgcacctcg taaccttttt ccagcagaaa ctctgccagg
    15721 taagaaccgt cttgtccggt tacaccggtg atgagagcga cttttgacat gtattattcc
    15781 tctgtatttt tgaatttatt cagtttcaac gcgttcgcgt atcaccactg cgggattccc
    15841 ccggcaaacc acatttgccg gaagcgattt aaaaacactg cttcgtgcac ccacgacggt
    15901 gccgtcgccg attgtgacgc ctggggcaac aaagacatcg gttgccagcc agcatttctc
    15961 gccaatcaca ataggcgtgg cgttaatggt gaaatgttga cttgcatggt cgtggctacc
    16021 ggtgcataaa taactttttt gcgatatcac cgaatgtgcg ccaatggtta tttcaccgag
    16081 ggtatataaa ttgacgtcat cgccgaccca cgcgtaatca cctaaggtta atttccacgg
    16141 ataggtaatt tttactgacg gacgaataac tacgtttttt cctatttttg ctccgaataa
    16201 acgtaataaa aaagcccgcc agcgatacaa tacttgtggc gaccaggcaa atattgttgc
    16261 ctgtactgcc caccataatt gcactttaat agcgttgccg ccccggaacc ctttcggcac
    16321 cgagaaaccg cttaaatctt gcattggttt tccttatatt cagactttgt tatataaggc
    16381 tttcgtcttt gaggtagtac gttggcgtaa atgccaggat aattcagccc aaaagccagg
    16441 cacatgtaat atttgtcgtt ggactttttt cgcgtcagca cacaattcca tattattggt
    16501 ggtagatacc ccacccatgg aaaattcaga caccaggcca ttgagttttt taaatgcata
    16561 accggcttta tacattttgg ctgccagcgc gtagtcggaa gaaactttat attccaggtc
    16621 ataacgccat tttttcaagc cggatacagg gaaaaatatc gcctgatgac tggcgggcag
    16681 gctgtgataa atataccagc ccggtttggc gctacgttta attttatgcc cgtcgccaaa
    16741 atccagcagc gcatcgccgg tgatcatcac gttatctttt tgcattttta acttacggac
    16801 aaaatttgcg gcattctgat gaaaaatatc gcccgaattg agaaacaacg cgaacttgcc
    16861 ttgtgccatc gcaatgcctt tgttcatggc gtcgtagata ccgttatctg gctcgctgac
    16921 aaagcgtagg ttaaagatac cattgagatt ttccagatac tcacgggtgc cgtcgttgga
    16981 accgccatcg acaacaatcc attcgaagct gatatcttcc acctgcgcca gatgcgccag
    17041 cgaggcatgt gttttgacta tcccttcgag gttacgaaac gcgacagtga ttatgctaag
    17101 caacatgttc ttattcttac ctcgtaatat ttaacgcttt tcgcaaaata aacggacaga
    17161 cgattaaaaa tgcatattcc gggctaaata tcgaaccggt aaaaaacagc gataccggag
    17221 taaaaagata taattgcacg cgaaaattgc ggttatcacc aaaagcgttg atcatcattt
    17281 ttatcacttt ccccatgtac cacagtgata aaaacaccgc gaaccaggaa aaataaataa
    17341 tcagcagata caaaccattg tctatggttt ttccgacatc cgcaccgtta aatattccga
    17401 atgatgcgac atattcataa agtgagccaa atctgactac accgtcaata tgggtcaaag
    17461 aataaccgac catcaccagc ggaccgacaa tacgataata tgatgacgat ccttccgtac
    17521 ctaaatcgcc cagacgggtg gaaatataag gaaacgcgat taccacacca accaggaata
    17581 cagccagaga aatcaatgct aacggtaact ttttcttaat cgcctcttta ttcagatatt
    17641 gaaatgccca ctccagcaaa taaaacagga taaaggtcat aacccctgaa aacgatccgg
    17701 ataatattat ccctgcgaga atcatagcat ctgttttagg cgttttgata ccaaactgtt
    17761 tgatgctgag ccaaattgag attaatgcca gagcgaaaaa tgccggttcg aaataaagtg
    17821 ctgtcgtgcg cttgccgccg aatttaatga aattcagcac atagctgttg ctgtaaatca
    17881 gatatttcga aatcgactcc ataatactgc tgccaccggt gaggataatt tgtgccatct
    17941 ccaccgccgc cagcgccacc accagcccga ccaccagata aaagaaacgt aatatcttgc
    18001 gatggttgtg tggcgaaatt gttttaaaac gaatactcca caccatgcca ataatgatca
    18061 caatatagac aaacagcata gttgaagtga cgtatttact ggcatccagc gactgaccaa
    18121 acagatagtt aaacgccgtg agtcccgcgc caatccctaa ggcaatcatc aattttttaa
    18181 cgctgatgcg ttctaaaaac agcagtaaca agacaggtaa aaaagtgacg atggtgatgg
    18241 ggaaactttc gccaagctgg gcgattttga cgttaaccag cagataaatc agcggcagca
    18301 acaggtagct acagattctg atagaagttg acatactcct ccagcatctg ttgtccactg
    18361 taggcggcgc ggctgcgttg gctgaactca gccagcgtgg taccaaatat cgcctgcgcg
    18421 atttccggtt tgcttaactg caccagttgc agcacctctt cttcgctgac ggttttaccg
    18481 ccggattttt gcaacacttc ccgcgccgca tcgctatggg tggcaatcac cggcacgcca
    18541 atcgatagcg cctcacacaa aatcagcggg tagttatcga cgcgagaact gaataccagc
    18601 gcatccatct gattgagcgc gctcatcagc ttacgtttgt cggtttcaaa gccgtgatta
    18661 accacgttgc cagcggtgaa cggcgagaac ttaccaaagg tatgcagttc gattttgtcg
    18721 cccagcgcca tcatctcacg caccagttgc tggttagttt tgccgtcgta acgcaggtca
    18781 tgcgccacca cagcgatttt cggcttgccc tgggtttcgc gcaccggagg cagatccgcc
    18841 agaatcgctt cggttgccat atcaatacca ttattgataa tccggcaacg ccctggaccg
    18901 tacaggctat tgaaagcgtc agccacatgc tggctggggg aaataaactg acagcccagc
    18961 gccagcatct cacggaataa ctggcgtttg cccgccacca gctggtgcgc ccgatcaatc
    19021 ttcaccggcg gatagttatt taaggtcggg catttctggc agcccgtttt ccagccttca
    19081 caaccgtcgg taaaggcgca gcgtccggta acgctccagt ggtcgtgcag cgtccagacc
    19141 agagtgacgt ccggtttatg gtttttcacc ttttcgcaaa agcgcaccac gctcttaaga
    19201 tttagccagt agctgtgcag cacatgaaaa tgcaggacca ccggacccgg tgtacgagta
    19261 atggtgcgat ataactcatt gaaattgcca aacagatcgc gattaaacag acgaaacagg
    19321 acaatattcg ccattgcagt catccgcggc gtatgtttga tgacctgcgg atagttttga
    19381 tggctgacgc tctctttgcc gcctttgccg taaccgtaga caaaatgtga cgctagcccc
    19441 tgttgcagcg cacgctggtg gagatctaac gccacacctg ctgccccgcc ttccgccagt
    19501 cgcacattaa attgcaaaat attcatttaa ttaccttcac tcgcgctttt tctcccacca
    19561 ccagcgcgtt gtccgggaca gagtcgagca ccacgctgcc cgcacccacg gtgacgttgt
    19621 taccaagcgt gatatcacca agaataataa cgttggcacc gagttcgacg ccgttgccaa
    19681 tatgtggaca tgccatgtta tcggcaccac gattgccgat agtgacgccg tggcgaatgg
    19741 tgaaatcatc ccccgccacc acgtttttat tgatcactac ggcgtaaccg tgatggatag
    19801 taaagcggcg gccaatggtc gcggcagcct ggatttcata accgaaaaag cattcggtga
    19861 taatgcgata cagcaccagc agaggggccg cccaaatatt gttgaggacg ttctttttgc
    19921 gccacaccga acaaaaatga gcgacgcgat acgctagaac catgcagcac ggacgtaaac
    19981 tccagctgtt ggcgcgcaga tcttccagca tctttagcgc ccccgaatac catcagccag
    20041 acgtttgccg ttacgcaccg acagcagcgt cagcaaagtg cgccaggtca tacgtttatt
    20101 gcggatctgg tagagggtaa acagctgata ttttttgctg gcgcggtcaa atttgtcttt
    20161 gtgcttgcgg taaaagtgga agtatccgga gaattttttc ggtgacgagg tgatctgcat
    20221 ttcaccgtga ttgatatgca ggatctgcgt cgcctcttcc actttccacg gttcgccgta
    20281 ctccaccacc atccgcagga agatgtcgta atcctgtgcc gctttcagtt cggtatcgaa
    20341 caaacactct ttgaaacgcc atgcccaggt aaagacctga ttgccaatga tattgcgttt
    20401 atagaacagg cggcgtgagt acggcgattt gggatacagt ggtaagcttg ccggttggga
    20461 ataaacttct ccctggcaaa cgtagtcgtt agcgtacaaa aaggcgtgag tgaccagttg
    20521 ctgtttatgg gcgaggaaga cgctcagacg gttgggtgtc cattcatcat catcatcgat
    20581 cccggtaatg tattcccctt gcgccagcat aatagcctgg ttacgtaccg cgcacgcccc
    20641 gctgttaatg tcgttgtgaa tgtaagtgat acgcggatcg ttgagggcgg tgacgtactg
    20701 ttgcagttgt tcccaggagg tggagcaatc atccacgatg atcatctccc agttgctgta
    20761 gtcctggcgc aagaccgatt ttatcgcccg aatcgccagt tgttggcggt tccaggtcgg
    20821 catatagatt gagatcagcg gattgtcttt cataggttgt tctcccggag tgtcgagatg
    20881 ttctgccggt ttataagctt gtttacgggt tctgttgccg gatgcggcgt gagcgcctta
    20941 tccggcctac ggagctggtt tgcttgccgg atgcggcgtc aacgtaggtc ggataagatg
    21001 cgtcagcatc gcatccgaca acggatctcg ttatttcgca tccgacttat attcgtattc
    21061 gtaatagcca taatcctgat acgcgctggc gcggcggaag atggagttca gaatcacccc
    21121 tttcaccgga ataccgtttt gctcaaagcg gctcagactg gtttccactt ctttcaatgt
    21181 gttgaccgca taacgcgcca ccattaatgt ggttccgaca tgacgaccaa caattgccgc
    21241 atcggtcact gccagaatcg gcggcgtatc aatcaacacc aggtcatagt ttttactcgc
    21301 ccagttcacc agttcggcaa agcgttcgct catcaacagt tcagaaggat ttggcggcac
    21361 ctgaccgcgc gggatcaggt caaatttggc aatagaggtc ggtttagctg cggtagtaat
    21421 atcgccctga ccaatcagaa tttccgacag gccattgacg ttattggtgc ccaacagctc
    21481 gtgggtgtag cctttgcgca tatcgcagtc gatcaacaac acgcgtttat tggtctggct
    21541 gatcaccgcc gccaggttgg cgcagacaaa ggttttacca attgacgggc taaccccggt
    21601 catcatcaac acattgttct gtgcctgcat catcgcgaag tgcaaactgg tacgcagact
    21661 acggatggct tcaatcgcca gatcggttgg attccccacc gccagtagct ggctctgttt
    21721 atagcgttta atccctttga tggttttgac gctatcacgc gctttctgcc attccgacag
    21781 cgggatgctg gcatagacgc taataccgtg ttcttccagt acttgcgggc tttcaatgcc
    21841 gcgattaaac aacgagcgca gcagcacacc cacgatagaa agcatcaggc caagaataat
    21901 cgccccaagg ataatcagcc ctttcttcgg ttttagcaca ccaggctgag tgattgccgg
    21961 gtcaacaatg cgcacatcgc cgacggtgct ggcttcggtg attttcagct cctgctcttt
    22021 attcagcagt tgcatataga cctgctgacc agactcgaca tcgcgggtca gacggacaat
    22081 ctcctgctgg gttttcggca tcgccgttac gcgaccgtta agtttggctt tttcgtcttc
    22141 cagcgcctga cgtttctcca gcagcgtgcg gtacgccggg tgaactttgg tgtacagctt
    22201 ggagatttcc gcctctttaa aggtcagttc gttcaactgg gcgtcgatgt tcaccatcga
    22261 atcgagcacc gctttcgctt ccagcggcag atcaacagaa tctttatcct gacggaaggc
    22321 attcagtttg ttttcggcaa catccaagcg gctacgtact tccggtaact gttgcgcgag
    22381 gaaggcgagg cttttcgacg cttccgccga tttgcgctca atattttgtt cctgatagtt
    22441 acgggcgatg ctgttaagaa tgtcgcggat ctgttcacga tcttcaccgg tataagtcag
    22501 gctcaaaacg ccagcgtctt tgccgttctc cgttaccgtc aggctgtttt gcagttggtt
    22561 gatcatcccc agcgtggagt atttggtgac ggtaaactca ctgcccgggc tggcgtgaat
    22621 ggcttcaacc atcagcgtga cgccttcttt tttcagcatc tggcccgctt gcccccgggc
    22681 gctaaagccg ccgtcgctgc tcagggtgta gtttttgttg tccagcacat taagcgtaaa
    22741 cacctgatcc gccatctctt tcgggcggtt aaaggtggtc actttcaccg tctcgttctg
    22801 acgtcccatc aggcgatccc agcccgcacc gaaaatcggg aacgtgtttt tgctcactgc
    22861 aatatcgagg tcgagatcgt ccaccgtttt accaagcacc agacgcgaac gaatcaactg
    22921 gatctcggcg tccgatgcag gcggtttgtt ggctaatgcc gagccaatgt cctgcactaa
    22981 cgaattgccg ctgttttgtt cgatttgtac cagtgcgtcg gcactataaa tcggcgtggc
    23041 gaagaaagtg taaaccacag cacagagggc aaatacagcg gtgatgccaa tcacccacca
    23101 gcgcgcttca atgacggtgc cgaccaggcg accaatatcg atttcatcac tgcccgttac
    23161 cggagcggca tgttgtttta ctttttctgt cattcttata cctgctctgc gttcaatgcc
    23221 tgcgcccact ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa cgtttcccgg
    23281 cttttgcgat acggatcggg gatttcacat tcgttatccc agtgaccaaa cagcatcact
    23341 ttgccgcgca tctcaggtgc catctcgcat aagcgttcga tatggcgctt ttccatggtc
    23401 aaaatcaggt catagttgcg acacaggcgg cggctgattt gccgtgcaca gtgaccttcc
    23461 agagacagtt gatgttctgc ggcgacgctg atagcggtag gatcagcgcc cttaccgacc
    23521 agcgcgccga gtccagcgga ctccactttc agctccgggt gataacgttg cagtaagcgt
    23581 tccgccgtcg gggaacggca aatattgccg acacagacaa ctaagatgtt gttaaacatg
    23641 acgattacca gttatgaatg tcgctggctg tatccgtcat gtaacggaca ccgctaatag
    23701 ttggcagcaa ctgattgatc agacggttcc agcgggaaac cggggcggtg gtgacataca
    23761 ccacgtcata aggttgcagg cggaattctg tcgccatcac cagcgacgtc gcatcggaca
    23821 tatccagctg gtagatattg gcaatcttgc cgttacgccc gccctcgcct ttcagcggac
    23881 gaatgacaaa gatgccgctg gcgttggagg tggtcatgtc gataccttcg gcattgccca
    23941 gggcttcagt cagggtcatg ccgctaaagt ccattttcag ggtgctctgt ttcttcactt
    24001 cacccatcac aaacactttc agatcatcat tacgcggcac gtagagaata tcgccggggt
    24061 aaagcagtcg gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa gaaatgcgtt
    24121 cttcacgacc attgtgtgtt agcaccacgt tgcgccagtc agcggtgtcg gtcaggccac
    24181 ctgcggcgtt gatggcgtcg agaatggtca gtggtacgtt ggtgatcgcc tgttgaccgg
    24241 atttattcac ctgacctgag atataggcct tttgcgagcg gaaggcggcg atattaacat
    24301 ccacctgcgg gtcagcgatg tacgtcgcta agcgcccggt aatatcactg cggatttcag
    24361 cgagcgtttt cccgactacg tggaccttgc cgatatacgg gtaaaacata gtgccgtcag
    24421 gctgtaccca gttgccggtg tcgctggagc tgcggtactg accggctggc gtggtgagtt
    24481 ccgggtgatc ccagacggtg acattcagaa cgtcgcccgg cccgacgcga tactggtaat
    24541 tcgcgatctc actttccagc gtcatattgg ggcgcgctac attcgggcgt gggcgtaatt
    24601 ggtcaatcag gcgcggggtc agcggataaa cattcaccat tttgtcgaga tcgaaatcag
    24661 cgtcctgctg tttgatgacg tctttgccca tcgtcgacat attgctgccc ggaagtactg
    24721 tgcaaccgct tatcaaggtt actgacacca ataatggcat caatttcatt ttggatttca
    24781 tcattgttta tttatcactt tggcagagta attatcctgt gcactattaa tagcaatgtc
    24841 gccatgcaca tttaccttgc agttaattga atacaaattt aactggcatc agtcctaaaa
    24901 aaattgattt catccgcagg ctattgacag aataattcag actggtcttt caggcatcca
    24961 gacacgctac cgcccctggc tttttagcta ccaatacact gatttagctt aatttttcaa
    25021 accctctctg catacaatcg ttgatgagaa agggttattg cggaaattaa cttccgaata
    25081 taaggtgaca ttatggtaat tgaatattgg ctctccaata atgcaggagg aagtgttaca
    25141 gctaacggaa tagcaggcaa ggtaacaatt cggcaattgg ctatttttaa gaattatatt
    25201 aagtgtcatt caatatggtt tttaggagtt tcttta
//
LOCUS       DAZFZC010000077        24949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFZC010000077 DAZFZC010000000
VERSION     DAZFZC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.077"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..681
                     /locus_tag="ACHKXP_003466"
     CDS             <1..681
                     /locus_tag="ACHKXP_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7648410.1"
                     /translation="HIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGC
                     GWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKV
                     QNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLR
                     LRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA
                     QQNSFKHT"
     gene            837..2372
                     /gene="gadC"
                     /locus_tag="ACHKXP_003467"
     CDS             837..2372
                     /gene="gadC"
                     /locus_tag="ACHKXP_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO7648411.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2503..3822
                     /locus_tag="ACHKXP_003468"
     CDS             2503..3822
                     /locus_tag="ACHKXP_003468"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO7648412.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4186..5568
                     /gene="dosC"
                     /locus_tag="ACHKXP_003469"
     CDS             4186..5568
                     /gene="dosC"
                     /locus_tag="ACHKXP_003469"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO7648413.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5593..7992
                     /gene="dosP"
                     /locus_tag="ACHKXP_003470"
                     /gene_synonym="pdeO"
     CDS             5593..7992
                     /gene="dosP"
                     /locus_tag="ACHKXP_003470"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO7648414.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEIQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDLGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8250..8831
                     /gene="ddpX"
                     /locus_tag="ACHKXP_003471"
     CDS             8250..8831
                     /gene="ddpX"
                     /locus_tag="ACHKXP_003471"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO7648415.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8845..10395
                     /locus_tag="ACHKXP_003472"
     CDS             8845..10395
                     /locus_tag="ACHKXP_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7648416.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALVTQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10397..11419
                     /locus_tag="ACHKXP_003473"
     CDS             10397..11419
                     /locus_tag="ACHKXP_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7648417.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11416..12311
                     /gene="ddpC"
                     /locus_tag="ACHKXP_003474"
                     /pseudo
     CDS             11416..12311
                     /gene="ddpC"
                     /locus_tag="ACHKXP_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
     gene            12308..13294
                     /locus_tag="ACHKXP_003475"
     CDS             12308..13294
                     /locus_tag="ACHKXP_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7648418.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13287..14213
                     /locus_tag="ACHKXP_003476"
     CDS             13287..14213
                     /locus_tag="ACHKXP_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO7648419.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14269..14700)
                     /gene="osmC"
                     /locus_tag="ACHKXP_003477"
     CDS             complement(14269..14700)
                     /gene="osmC"
                     /locus_tag="ACHKXP_003477"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO7648420.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15045..15260
                     /gene="bdm"
                     /locus_tag="ACHKXP_003478"
     CDS             15045..15260
                     /gene="bdm"
                     /locus_tag="ACHKXP_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO7648421.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15362..15499
                     /gene="sra"
                     /locus_tag="ACHKXP_003479"
     CDS             15362..15499
                     /gene="sra"
                     /locus_tag="ACHKXP_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO7648422.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15656..17353
                     /gene="maeA"
                     /locus_tag="ACHKXP_003480"
     CDS             15656..17353
                     /gene="maeA"
                     /locus_tag="ACHKXP_003480"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7648423.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGMV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17487..18497
                     /gene="adhP"
                     /locus_tag="ACHKXP_003481"
     CDS             17487..18497
                     /gene="adhP"
                     /locus_tag="ACHKXP_003481"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO7648424.1"
                     /translation="MKAAVVTKDHHVDVTYKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSHTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQNLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18643..18927
                     /locus_tag="ACHKXP_003482"
     CDS             18643..18927
                     /locus_tag="ACHKXP_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7648425.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19156..19809)
                     /gene="fdnI"
                     /locus_tag="ACHKXP_003483"
     CDS             complement(19156..19809)
                     /gene="fdnI"
                     /locus_tag="ACHKXP_003483"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO7648426.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19802..20686)
                     /gene="fdnH"
                     /locus_tag="ACHKXP_003484"
     CDS             complement(19802..20686)
                     /gene="fdnH"
                     /locus_tag="ACHKXP_003484"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO7648427.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20699..23746)
                     /gene="fdnG"
                     /locus_tag="ACHKXP_003485"
     CDS             complement(20699..23746)
                     /gene="fdnG"
                     /locus_tag="ACHKXP_003485"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23159..23161),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7648428.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYTPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23978..24859
                     /gene="yddG"
                     /locus_tag="ACHKXP_003486"
     CDS             23978..24859
                     /gene="yddG"
                     /locus_tag="ACHKXP_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO7648429.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATHHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMIFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6086 a         6334 c         6321 g         6208 t
ORIGIN      
        1 cacatcgacg ctgccagcgg tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg
       61 gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt cggtctggct
      121 ccgctgggct gcggctgggt tatctggcgt gacgaagaag cgctgccgca ggaactggtg
      181 ttcaacgttg actacctggg tggtcaaatt ggtacttttg ccatcaactt ctcccgcccg
      241 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggctatacc
      301 aaagtacaga acgcctctta ccaggttgcc gcttatctgg cggatgaaat cgccaaactg
      361 gggccgtatg agttcatctg tacgggtcgc ccggacgaag gcatcccggc ggtttgcttc
      421 aaactgaaag atggtgaaga tccgggatac accctgtacg acctctctga acgtctgcgt
      481 ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg aagccaccga catcgtggtg
      541 atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg ctgaactgtt gctggaagac
      601 tacaaagcct ccctgaaata tctcagcgat cacccgaaac tgcagggtat tgcccaacag
      661 aacagcttta aacatacctg ataacgttta acggtaacgg tgtcccgaaa cgaacccgtt
      721 tcgggacaat ttccaaagtc tgttcactgg cattagcaac ggaaaatatt gttctgaata
      781 cgcttcagaa caaaacaggt gcggttccga caggaatacc gttttagggg gataatatgg
      841 ctacatcagt acagacaggt aaagctaagc agctcacatt acttggattc tttgccataa
      901 cggcatcgat ggtaatggct gtttatgaat accctacctt cgcaacatcg ggcttttcat
      961 tagtcttctt cctgctatta ggcgggattt tatggtttat tcccgtggga ctttgtgctg
     1021 cggaaatggc caccgtcgac ggctgggaag aaggtggtgt cttcgcctgg gtatcaaata
     1081 ctctggggcc gagatgggga tttgcagcga tctcatttgg ctatctgcaa atcgccattg
     1141 gttttattcc gatgctctat ttcgtgttag gggcactctc ctacatcctg aaatggccag
     1201 cgctgaatga agaccccatt accaaaacta ttgcagcact catcattctt tgggcgctgg
     1261 cattaacgca gtttggtggc acgaaataca cggcgcgaat tgctaaagtt ggcttcttcg
     1321 ccggtatcct gttacctgca tttattttga tcgcattagc ggctatttat ctgcactccg
     1381 gtgcccccgt tgctatcgaa atggattcga agaccttctt ccctgacttc tctaaagtgg
     1441 gcaccctggt agtatttgtt gccttcattt tgagttatat gggcgtagaa gcatccgcaa
     1501 cccacgtcaa tgaaatgagc aacccagggc gcgactatcc gttggctatg ttactgctga
     1561 tggtggcggc aatctgctta agctctgttg gtggtttgtc tattgcgatg gtcattccgg
     1621 gtaatgaaat caacctctcc gcaggggtaa tgcaaacctt taccgttctg atgtcccatg
     1681 tggcaccaga aattgagtgg acggttcgcg tgatctccgc actgctgttg ctgggtgttc
     1741 tggcggaaat cgcctcctgg attgttggtc cttctcgcgg gatgtatgtc acagcgcaga
     1801 aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa tggcgtaccg gtaacgctgg
     1861 tcatttcgca gctggtgatt acgtctatcg cgttgatcat cctcaccaat accggtggcg
     1921 gtaacaacat gtccttcctg atcgcactgg cgctgacggt ggtgatttat ctgtgtgctt
     1981 atttcatgct gtttattggc tacattgtgt tggttcttaa acatcctgac ttaaaacgca
     2041 catttaatat ccctggtggt aaaggggtga aactggtcgt ggcaattgtc ggtctgctga
     2101 cttcaattat ggcgtttatt gtttccttcc tgccgccgga taacatccag ggtgattcta
     2161 ccgatatgta tgttgaatta ctggttgtta gtttcctggt ggtacttgcc ctgcccttta
     2221 ttctctatgc tgttcatgat cgtaaaggca aagcaaatac cggcgtcact ctggagccaa
     2281 tcaacagtca gaacgcacca aaaggtcact tcttcctgca cccgcgtgca cgttcaccac
     2341 actatattgt gatgaatgac aagaaacact aaccatgtct gaatggttat aagacaaggg
     2401 agcgataatt catcgctccc tttttcgtgc ttgctgtcgt attgacttaa cggggataag
     2461 tacgagaatg agcgcacatc tgtttaccgg aaaccagcac atatggatat ctgctcccga
     2521 aacaagaaat taacgattag aagaccagcg atactagttg cactggcact tttactgtgt
     2581 agttgtaaaa gcacgcctcc agagtccatg gtgacaccac cagcaggttc aaagccacca
     2641 gccacgacgc aacaatcgtc acaaccgatg cgtggcatct ggctggccac ggtttctcgg
     2701 ctcgactggc caccggtttc ctcggttaac attagtaacc ccaccagccg ggcccgtgta
     2761 caacaacagg cgatgatcga caaactggat catctgcaac gtctcggcat aaacacggtc
     2821 tttttccagg tcaagccgga cggtaccgcc ctgtggccat cgaaaatttt gccgtggtcc
     2881 gatcttatga ccggtaagat tggtgaaaat ccgggttacg atccgctgca atttatgctc
     2941 gacgaagccc acaagcgtgg gatgaaagta cacgcctggt ttaaccccta tcgcgtatcg
     3001 gttaatacga agcccggtac tatcagggaa ctgaatagca ctctgtctca acaaccggcg
     3061 agcgtctatg tgcaacaccg cgactggatc agaacgtctg gcgatcgctt tgtcctcgac
     3121 ccgggcatcc ctgaggttca ggactggatc acatcaatag tcgcagaagt ggtttcccgc
     3181 tatcccgtag atggcgtgca gtttgacgac tatttctata cggagtcacc gggttcacgg
     3241 ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat ttgcgtcaaa agcagactgg
     3301 cggcgcaaca atactcagca gttaattgca aaggtatcgc acaccattaa aagcattaag
     3361 ccgggagtcg aatttggtgt tagcccggca ggcgtgtggc gtaaccgatc acacgatccg
     3421 ctcggttccg atacccgagg cgcggcagcc tatgacgaat cctacgctga cacccgtcga
     3481 tgggtggaac aaggattgct ggattacatt gctccccaaa tttactggcc gttctcacgg
     3541 agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg atgtcgttaa accgaccagg
     3601 acccgcctgt atatcggtat cgccttctat aaagtgggtg aaccttcaaa gatagagcca
     3661 gactggatga ttaatggcgg cgtaccggaa ctgaaaaagc agctcgatct taacgatgct
     3721 gtgcccgaaa ttagcggcac catcttgttc cgtgaggact atctgaataa accgcagact
     3781 caacaagcgg tcagctatct gcaaagtcgc tggggcagtt aaactcccat tcttactttg
     3841 taacaagccg gtgctttacc caccggcttg ttaccctctt aaactatccc actaataatc
     3901 atgcttactt aagtcaaatt aaccacactt agttaaacag ccataaatcc ctaataaaaa
     3961 tgtatgaata tgaaaaacca tacgatattc gctgtcaccg aaaaataatt acgatgggct
     4021 atactcttct gaagaacagt agcgtagatt gaaactgtcc taaatcgctt atttcttttc
     4081 agtatatctt catatttcag gagaatatgg tgcagttagt cgatctcgca cgttgcgttt
     4141 cattttgatt ccgtctgatt tcccagcctt ataagggtgt gaattatgga gatgtatttt
     4201 aaaagaatga aagatgagtg gaccggactt gtcgaacagg cagatccgcc cattcgtgct
     4261 aaagccgcgg aaattgccgt tgcgcatgct cattatctga gtatcgagtt ttatcgaatt
     4321 gtccgcatcg acccgcatgc cgaagaattc ttgagtaatg aacaagttga gcggcagttg
     4381 aagagtgcga tggaacgctg gattattaac gtgctttctg cccaggttga cgatgtcgaa
     4441 aggctaatac aaatccagca taccgtcgcg gaagtgcatg cccgcatagg aattccggta
     4501 gaaattgtcg agatggggtt tcgggtgctg aaaaaaatcc tctatccggt gatcttctct
     4561 tcggattatt ccgccgcaga aaaacttcag gtctaccatt tctcgattaa cagtattgat
     4621 atcgcgatgg aagtgatgac tcgcgcgttt acctttagtg acagtagtgc ctcaaaggaa
     4681 gatgaaaact atcgtatctt ctcgttactg gaaaacgccg aagaagaaaa agaacggcaa
     4741 atagcctcaa tactttcatg ggaaatagat attatctata aaatcctgct ggattctgat
     4801 ttaggcagta gtttgccttt aagccaggct gattttggcc tgtggtttaa ccataaaggt
     4861 cgacattatt ttagtggcat tgccgaagta ggccatatct cccgtctgat tcaggatttc
     4921 gacggtattt tcaatcaaac catgcgtaac accaggaatt tgaataacag aagtctgcgg
     4981 gtgaaatttt tattacagat aagaaatacc gtatcgcaaa ttattacctt gctgcgtgaa
     5041 ttgtttgaag aagtatcgcg ccacgaagtc ggtatggatg tactgacgaa attacttaac
     5101 cgccgtttcc tgccgactat cttcaaacgc gaaattgccc atgccaaccg gaccggtaca
     5161 ccgctgtcag tgctgattat tgacgttgat aaattcaaag agatcaacga tacgtggggc
     5221 cataacactg gcgatgaaat tctgcgtaaa gtctctcagg ccttttatga caacgtccgc
     5281 agtagtgatt atgttttccg ctacgggggc gatgaattta tcattgtttt gactgaagct
     5341 tctgaaaacg aaacgttacg taccgcagaa cgtattcgca gtcgggtgga gaaaaccaaa
     5401 ctgaaagccg caaacggcga ggatattgcc ctctcacttt ccatcggtgc cgccatgttt
     5461 aatggtcatc ctgactatga gcgcctcatt caaatagccg atgaagctct gtatatcgcc
     5521 aaaagacgag gtagaaaccg tgttgaactc tggaaagcca gtctttagat gcgccaggat
     5581 gcagaggtaa tcatgaagct aaccgatgcg gataatgccg ccgatggcat ttttttcccc
     5641 gcccttgagc aaaatatgat gggtgcggtg ttaattaacg aaaatgatga agtgatgttt
     5701 ttcaaccccg ccgcagagaa gctctggggg tacaaacgtg aagaagtcat tggcaataac
     5761 attgatatgc tgattccgcg ggatttgcgt cctgcgcatc ctgaatatat tcgtcacaac
     5821 cgtgaaggcg gtaaagcgcg cgtcgagggg atgagtcggg agctgcagct ggagaaaaaa
     5881 gacggcagta aaatctggac ccgttttgcg ctatcgaaag tgagcgccga ggggaaagtt
     5941 tattacctgg cgctggtacg ggatgccagc gtagaaatgg cgcaaaaaga acagacccga
     6001 caattgatta ttgccgttga ccatctcgac cgaccggtga ttgtcctcga tccggaacgc
     6061 catattgtgc agtgcaatcg cgcatttacc gaaatgtttg gttactgcat tagcgaagcc
     6121 agcggtatgc agcccgatac actcctgaac attcctgaat tccctgccga taaccgcatt
     6181 cgtttacaac agttgctatg gaaaaccgcc cgcgatcagg acgaatttct gctgttgacg
     6241 cgcaccggtg aaaaaatctg gattaaagcc tctatcagcc cggtttatga cgtgctcgcg
     6301 catctgcaga acctggtaat gactttctcg gatatcaccg aagaacggca aatccgccag
     6361 cttgaaggta atattctcgc cgccatgtgc agcagcccgc catttcatga aatgggggaa
     6421 atcatttgtc gtaacatcga atctgtactc aacgaatcgc atgtttcgct gttcgcactg
     6481 cgcaacggga tgccgataca ctgggcgtca tcttcccacg gtgcagaaat tcaaaatgcg
     6541 caaagctggt cagcgaccat tcgtcagcgt gatggcgcgc ctgcggggat cctgcaaatt
     6601 aaaacctcgt caggagcaga aaccagcgcc tttatcgaac gcgtggcaga tatcagccag
     6661 catatggccg cgctggcgct ggaacaggaa aaaagccgtc agcatattga acaactcatc
     6721 caatttgatc cgatgaccgg tctgccaaat cgcaataacc tgcacaatta cctcgatgac
     6781 ctggtcgaca aagccgtctc tcccgtggtg tatctcatcg gtgttgacca tattcaggat
     6841 gtgattgata gccttggcta tgcgtgggcc gatcaggcat tgctggaagt ggtcaatcgc
     6901 tttcgtgaaa aactcaaacc ggatcagtat ctctgtcgta tcgaaggtac gcagtttgtc
     6961 ctcgtgagcc tcgaaaacga cgtcagtaac attacccaaa tcgccgatga gctacggaat
     7021 gtggtcagca agccgataat gattgacgat aaacccttcc cgcttacctt gagtattggc
     7081 atcagctacg acctgggtaa aaaccgcgat tacttgctct ccactgctca caatgcaatg
     7141 gattatattc gcaagaatgg cggtaacggc tggcagttct tcagcccggc gatgaacgaa
     7201 atggtaaaag agcgtttggt tttaggcgca gcgctgaaag aagcgattag caataaccaa
     7261 ctgaaactgg tttaccagcc gcaaatcttc gcagaaacgg gtgaactgta cggcatcgaa
     7321 gcccttgctc gctggcacga tcccctgcat ggtcatgtgc ccccttcacg gtttattcct
     7381 ctcgcagaag agattggtga aatcgaaaat attgggcgct gggtcatcgc ggaagcttgc
     7441 cgtcagttag cagaatggcg tagccagaat attcatatcc cggcgttatc cgtgaacttg
     7501 tcggcgctgc actttcgcag taatcaactg cctaatcagg tgtctgatgc aatgcacgcc
     7561 tggggtattg acggccacca gctgacggta gaaatcacgg aaagcatgat gatggaacac
     7621 gataccgaaa tctttaagcg cattcagatc ctgcgtgata tgggcgtggg cttatcggta
     7681 gatgattttg gtacgggctt ttccggatta tcccgcttag tcagtcttcc ggtaacggaa
     7741 atcaaaattg acaaaagttt tgtcgatcgt tgtctgaccg aaaaacgcat ccttgcctta
     7801 cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa ccgtcgtggc ggaaggcgtc
     7861 gaaaccaaag agcaatttga gatgctacgc aagatccact gtcgcgttat tcagggatat
     7921 ttcttttccc gccccctacc cgccgaagaa attccaggct ggatgagcag cgtgttaccg
     7981 ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt tttcatttaa cgtgacactg
     8041 tcacttaaat actgtgattt cagctacgat tccgggagat tccttcctta acttcccctc
     8101 ttgcaccaaa actgcactac gctgcacaaa tgtttatcaa aagttaaaaa atataacttt
     8161 aagccagcat tctggcgctt atcccggcat ggcatgagat ctgcataagc ggaaagcgca
     8221 gcaatttttg tcttatacag gaaccgttta tgtcggatac caccgaactg gttgatttag
     8281 ccgtaatctt ccctgatctg gagatcgaat tgaaatacgc ctgcgctgat aacatcacag
     8341 gtaaagctat ttatcagcaa gcgcgttgtc tgttacacaa ggatgcgatt accgcgctgg
     8401 cgaaaagtat cagcatcgcc cagctgtcag ggttacaact ggtgatttac gatgcgtatc
     8461 gcccacaaca agcacaggcg atgttgtggc aagcctgccc agacccgcaa tatgttgttg
     8521 atgtgacggt cggttctaat cacagccgtg gcacggcgat cgacctgacg cttcgtgatg
     8581 agcacgggaa catcctcgat atgggcgcag ggttcgatga aatgcacgag cgctcccatg
     8641 cctatcaccc ttccgtcccg cccgccgctc agcgcaatcg gctgttgctg aatgcgataa
     8701 tgactggtgg cggttttgtc ggtatctcca gcgaatggtg gcacttcgaa ttaccccagg
     8761 cagcgagtta ccctctgctc gctgatcaat tctcttgttt tatatcccct ggcacacagc
     8821 acgtcagtta atcaggaaac tgtcatgaag agatcgatat cgtttcgtcc cacattgctc
     8881 gcgctcgtcc ttgccacaaa tttcccggtt gcgcacgccg ccgtaccaaa agatatgctg
     8941 gtgattggta aggccgccga tccacaaacc ctcgacccgg cggtaacaat agataataac
     9001 gactggacag tgacctaccc gtcttatcag cggctggttc agtacaaaac ggacggtgat
     9061 aaaggctcaa ccgacgttga aggcgatctg gcaagtagct ggaaagcgtc tgacgatcaa
     9121 aaagagtgga cgttcaccct gaaagataat gctaaatttg ccgatggcac acctgtcact
     9181 gccgaagcag taaaactttc ttttgagcgg ctactaaaaa tcggccaggg gccagcagaa
     9241 gcatttccca aagatttaaa gattgatgct cccgacgaac atacggttaa gtttaccctt
     9301 agccaaccat tcgcaccgtt cctctacacg ctggcgaatg acggtgcatc cattatcaat
     9361 ccggcggtct taaaggaaca tgcagcggat gatgctcgcg gcttcctcgc gcaaaatacc
     9421 gccggttccg gaccatttat gctgaaaagc tggcaaaaag gtcagcaatt agttctggtg
     9481 ccaaatccgc attacccagg caataaaccg aacttcaaac gggtatcggt aaaaattatt
     9541 ggtgaaagtg cctcccgtcg cctgcagctc tcccgtggcg acattgacat tgccgatgcg
     9601 ctgccggtgg atcaactcaa cgccctgaag caggaaaata aagtcaatgt ggcagagtat
     9661 ccgtcactgc gcgttaccta tctgtatctg aataacagca aagcgccact taatcaggcg
     9721 gatctgcgtc gggccatttc ctggtctacc gattatcagg gcatggttaa cggcattctg
     9781 agtggtaacg gaaaacagat gcgcggcccg attccggaag gcatgtgggg ctacgatgcg
     9841 acggcaatgc aatacaacca tgacgaaacg aaagccaaag ccgaatggga taaagtgacg
     9901 agcaaaccca ccagcctgac gtttctctac tccgataacg atccgaactg ggagcctatt
     9961 gctctggtga cacaatccag tctcaacaag ctgggcatca atgtgaagct ggaaaagctg
    10021 gcgaacgcca ccatgcgcga cagagtgggt aaaggtgatt acgacattgc gattggcaac
    10081 tggagtccgg attttgccga cccgtatatg tttatgaatt actggtttga gtcagacaaa
    10141 aaaggtctgc cgggtaaccg ctcgttctat gaaaacagtg aggtcgataa gttactgcgc
    10201 aatgcgcttg cgaccaccga ccagacgcag cgtacccggg actaccagca ggcacagaaa
    10261 atcgtcattg atgacgctgc ttatgtgtac ctgttccaga aaaactacca actggcgatg
    10321 aacaaagagg tgaaaggctt tgtgttcaat cccatgctgg aacaggtctt caatatcaat
    10381 accatgagta aataacgtga ccttctggag tattttacgc caacgctgct gggggctggt
    10441 gctcgtggtg gcgggcgtct gcgtgattac gtttattatc tcgcatctga tccctggcga
    10501 tccggcgcgg ttactggcgg gtgaccgcgc cagcgatgct atcgtggaaa atattcgcca
    10561 gcaactggga ctggaccagc cactgtatgt acagttttac cgctacgtca gcgacctgtt
    10621 tcatggtgac ctgggaacat ccattcgtac cgggcgtccg gtgctggaag agttgcgtat
    10681 atttttcccg gcgacgctgg aactggcttt tggtgccctg ctgctggcac tcctgattgg
    10741 catcccgctg ggcatactct ctgcagtctg gcgaaatcgc tggctggatc atctggtgcg
    10801 aataatggcc attaccggaa tctccacacc tgcgttctgg cttgggctgg gcgtcattgt
    10861 gctgttttat ggtcatctgc aaattcttcc cggcggcgga aggcttgatg actggctgga
    10921 tccaccaacg cacgttaccg gcttttatct gctcgatgcg ctgcttgaag gcaacggtga
    10981 agtcttcttc aatgcgttgc aacatctcat cttaccggca ttaacgctgg cgttcgttca
    11041 cctgggaatt gtcgctcgcc agatccgctc agcgatgctg gaacaattga gtgaagacta
    11101 cattcgtacc gcccgggcca gcggcttgcc cggctggtat atcgttttat gttatgcgct
    11161 acccaatgcg ttgatcccat cgattaccgt attgggtttg gcgctgggcg atttgttgta
    11221 tggcgcagtg ctcaccgaaa ccgtttttgc ctggcccgga atgggtgcat gggtagtaac
    11281 atcaatacag gcgctcgact tcccggcagt gatgggcttt gccgtcgtgg tttcatttgc
    11341 ttatgtgctg gtcaacctgg tggtggattt gctctatttg tggattgatc cgcgtatcgg
    11401 acgtggaggt ggtgaatgat gctaagcgag gaaacgtccg ccgtacgccc acaaaaacaa
    11461 acgcgattta acggtgcaaa actggtatgg atgctgaaag gcagtccgct caccgtgacc
    11521 ggcgcagtca tcattgtatt aatgctactg atgatgattt tttcaccgtg gctggcgacg
    11581 catgatccca acgccattga tttaaccacc cgcctttgcc gccttctgcg gcgcactggt
    11641 ttggcaccga tgaagtggga cgcgatctgt ttagccgcgt actggtcggc agtcagcaat
    11701 caattctcgc cggattagtc gtagttgcca ttgcgggtat gattggttcg ctactcggat
    11761 gtctatccgg tgtgcttggc ggacgcgcag acgccatcat catgcgcatc atggacatta
    11821 tgctgtcgat tccttcgttg gtactgacaa tggcactggc tgccgctctc gggccgagtt
    11881 tgtttaacgc catgctggcg attgctatag tgcgaattcc gttttatgtg cgcctggcgc
    11941 gggggcaagc attagttgta cgccagtata cctatgttca ggcggcgaaa acctttggcg
    12001 cgtcgcgttg gcatctgatc agctggcata ttttacgtaa ttccctgccg ccgctgatcg
    12061 tgcaggcatc gctggatatc ggtagcgcga ttttaatggc cgccacgttg ggatttattg
    12121 gcctgggtgc tcaacaaccg agtgctgaat ggggcgcgat ggtagcaaat ggtcgcaact
    12181 atgtactcga tcagtggtgg tattgcgcat ttccgggggc agcgattttg cttaccgccg
    12241 tcgggtttaa tctctttggt gatggtattc gcgatctgct tgacccgaaa gcaggaggaa
    12301 agcagtcatg acccaacccg ttctggacat tcaacaactg catttgagtt tccccggttt
    12361 taacggtgat gttcacgcgc tcaacaatgt gtccttgcag attaaccgcg gtgaaattgt
    12421 cggtctggtg ggagaatccg gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct
    12481 gctaccgacg ggcagttatt gcgtacatcg ggggcagatt tccctgctgg gagaagatgt
    12541 tcttaacgcg cgggaaaaac agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt
    12601 tcaggaaccg atgaccgccc tcaatccgac acgtcgaata ggtcttcaga tgatggacgt
    12661 gatccgccat catcaaccaa taagtcgtcg ggaagccaga gctaaagcga ttgacctgct
    12721 ggaagagatg caaatcccgg atgccgtgga agttatgtcg cgctatccgt ttgagctttc
    12781 aggtggtatg cgccagcggg taatgattgc gctggcattc tcctgcgagc cgcaattgat
    12841 tattgccgac gaaccgacta cggcgctgga cgtcacggta cagttgcagg tactgcgttt
    12901 gcttaaacat aaagctcgag ccagtggaac tgcggtgctg ttcatcagcc atgatatggc
    12961 cgtggtgtcg caactgtgcg atagcgttta cgtgatgtat gccggaagcg tgattgagag
    13021 cggcgtgacg gcagacgtta tccatcatcc ccggcatccg tataccattg gtttgctgca
    13081 atgcgcacca gaacatggaa taccgcgcca gccattaccc gccattccag ggacggtacc
    13141 aaatctcacc catttgcctg acggctgcgc ttttcgcgat cgttgttatg cggcaggtgc
    13201 acagtgtgaa aacgtcccgg cgctgacagc gtgtggtgac aacaaccagc gctgcgcctg
    13261 ttggtatccg cagcaggagg tcataagtgt ctgacacgtt attaacgtta cgcgacgtcc
    13321 atatcaattt cccggcccgt aaaaactggc ttggtaaaac tacggaacat gttcatgcga
    13381 ttaatggtat tgatttacag atccgccgtg gtgaaacctt agggatcgtc ggcgagtcag
    13441 gctgcggcaa aagcaccctc gcacagcttt taatgggtat gctgcaaccg agccacgggc
    13501 agtacatccg ttctggctca caacgcatta tgcagatggt gtttcaggac ccgctctctt
    13561 cgcttaatcc gcgcttaccg gtgtggcgca tcatcacaga accgctctgg atagctaagc
    13621 gtagtagtga acaacagcgg cgagcgttgg cagaggagtt ggctgtgcag gtgggtattc
    13681 gtccggagta tctcgaccgc ctgcctcatg cgttctccgg cgggcagcgg caacgtatcg
    13741 ccattgccag agcactctct tcgcagcctg acgtgattgt gcttgatgag ccaacctctg
    13801 cgctggatat ctccgttcag gcgcagatcc tcaatttact ggtaacgcta caggaaaatc
    13861 acgggctgac ctatgtgctg atttcacaca atgtctcggt gatacgtcat atgagcgatc
    13921 gggtggcggt gatgtatctc gggcagattg tagaactggg cgacgcgcag caggtgctga
    13981 cggcacctgc acatccatac acccgattat tgctggattc cctccccgcc attgataaac
    14041 cgctggagga agaatgggca ttacgtaaaa cggatctgcc gggaaaccgc acgttgccgc
    14101 aaggctgttt tttctacgaa cgttgcccgc tggcaaccca cggatgtgaa gtccggcaat
    14161 cattagcaat aagggaggac ggacgtgagc tccggtgctg gcgggcgctg tagagatgct
    14221 aacaacgcat caggcattca cgcgcctgac gcgtcatccg gcaatgcttt acgatttcaa
    14281 ctggtaatcc agcgtaattt ccgctttcag tacctgagag accgggcatc ctgcttttgc
    14341 tttctggatt atgccgtcaa aggtagaggc atcaataccc ggcaccgcaa cttcactctt
    14401 cagtgcgatt ttcgtaatcg caaaaccggc atccacttta tccagcgaca catcggcggt
    14461 ggtatcaatc gatgttggcg tgaatcccgc ttcccccagc attaatgaaa gcgccattga
    14521 gaaacatgcg gcatgcgctg cgccaatcag ttcttcaggg ttggttcctt tttcgccttc
    14581 aaaacgcgtg ttaaatccat acggctgttg gttcagcacg ccactctcgg tggatactgt
    14641 tcccttcccg cgtttgatat cgccttccca gtgtgcctga cctttcttat ggattgtcat
    14701 tgttgctctc ctgtgggctt aaaaaggaaa ttatagacta cgtgagaaac agcacgagga
    14761 taagcaggat attccgaata aaatccggcg aaaatattac ttctgcactt aaattaaatt
    14821 cccacaatag attaccggga atatataacc caaaagatat tgtatcaata ttaatcggat
    14881 cataccaatc acaatcccta tgaatatcat ggtgttaaaa tataaaaatc atcaaccagg
    14941 actaatctta acaacgaagc ggcaaatatt tgccatgctg aatgtgcttt ataaatctgc
    15001 gatccgtagc agacaccata aatacacaga cacggagaat cactatgttt acttattatc
    15061 aggcagaaaa ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg
    15121 cacagcatgg tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggttgaag
    15181 cttctgataa cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg
    15241 gccagcaccc tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc
    15301 acgtgtttct tcgctacggc gatgttgtcc ttaaaactag ctacaggatt gaggagttaa
    15361 aatgaaatcg aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca
    15421 gcgcaaaata gttaccgaag gtgacaaatc cagcgtagta aataacccaa ccggcagaaa
    15481 acgccccgct gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc
    15541 ctgcgcaatg gtgtttttgt ttttatctgc tttatacttg aggccgacgc cctggcggta
    15601 aagcaaagac gataaaagcc ccccagggat ggatattcaa aaaagagtga gtgacatgga
    15661 accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga
    15721 atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct
    15781 gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat
    15841 ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga
    15901 cactaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt
    15961 tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc
    16021 acgcggcgtg tttatctctt accagaaccg gcacaatatg gacgatattc tgcaaaacgt
    16081 gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg
    16141 tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg
    16201 tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa cgaacaacca
    16261 acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga
    16321 atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct
    16381 gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa
    16441 tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact
    16501 gatcgcagca agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg
    16561 tgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg
    16621 attaagcgag gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcttgctgac
    16681 cgacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct
    16741 cagtgactgg gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa
    16801 accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg
    16861 tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg
    16921 cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac
    16981 tggcagcccg tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa
    17041 caacgccttt attttcccgg gcatcggtct gggtgttatt gcttccggcg cgtcacgtat
    17101 caccgatgag atgctgatgt cagcaagtga aacgctggcg cagtattcac cattggtgct
    17161 gaacggcgaa ggtatggtac tgccggaact gaaagatatt cagaaagtct cccgcgcaat
    17221 tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga
    17281 agccctgcaa caggccattg acgataattt ctggcaagcc gaataccgcg actaccgccg
    17341 tacctccatc taagcctgcg cccggtagtg aaggctaccg ggctatttcc ctctcccttt
    17401 ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca cccgctatgc
    17461 agaacatcat ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg
    17521 ttgacgttac gtataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg
    17581 agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa
    17641 ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc ccaggtgtca
    17701 cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga tgcggtcatt
    17761 gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca
    17821 gcgttgatgg cgggatggcg gaagagtgca tcgtggtcgc cgattacgcg gtaaaagtgc
    17881 cagatggtct ggactcggcg gcggccagca gcattacctg tgcgggggtc accacctaca
    17941 aagccgttaa gctgtcaaaa attcgtccag ggcagtggat tgctatctac ggtcttggcg
    18001 gtctgggtaa cctcgccctg caatacgcga agaatgtctt taacgccaaa gtgatcgcca
    18061 ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggcgcagat ttagcgatta
    18121 actcacacac cgaagacgcc gccaaaattg tgcaggagaa aactggtggc gctcacgctg
    18181 cggtggtaac agcggtagct aaagctgcgt ttaactcggc agttgatgct gtccgtgcag
    18241 gcggtcgtgt tgtggctgtc ggtctaccgc cggagtctat gagcctggat atcccacgtc
    18301 ttgtgctgga tggtattgaa gtggtcggtt cgctggtcgg cacgcgccag aatttaactg
    18361 aagccttcca gtttgccgcc gaaggtaaag tggtgccgaa agtcgccctg cgtccgttag
    18421 cggacatcaa caccatcttt actgagatgg aagaaggcaa aatccgtggc cgcatggtga
    18481 ttgatttccg tcactaagag gcctttgctg cgactgccat gttcgggtcg cagcatcgca
    18541 cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa caataatgat
    18601 agtgttccca gtgaaggaac gctactgaga gtaaagacac taatgaaaat ggccaatcat
    18661 ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag cctgcgcgag
    18721 tttgccagag caatggaaat tgctccctca acggcaagcc gattgctgac aggaaaagca
    18781 gctttgacgc cagaaatggc gattaaactt tccgtggtga tcggcagttc gccgcaaatg
    18841 tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacagt ggatgtgtcg
    18901 cgattacgcc gtctggttac gcaataaaaa aatggcactt tataaagcgc catttagaac
    18961 tgatgacaaa cgcaacattg cctgatgcgc tacgcttatc aggcctacac gttcactgca
    19021 atctattgga tttgaagggt tttcatgccg gataaggcgt tcacgccgca tccggcatga
    19081 acaaagccta cgttgtctac aatatgaaaa tggcgccttt tacagcgcca ttttcagctt
    19141 aaagttcaaa agagattata tcccttcttc actctctttt ttcgcttctg ccttctcgat
    19201 ttcacgatac cagcgcggat ggtgtttctt cgcccagcga cgacttacct tcccttcgat
    19261 catcccttta atcgatcctt tcacccaaaa tgccatatac atatggatca ggatggcgtg
    19321 gatcaggatg atacccgcag ccgcgtggat cagcaggctg tagcgaacaa cctgcatcgg
    19381 gaagtactgc gcaaagtacg gacgccagat aatcaccccg gtcaccagca gcacgaaaat
    19441 catgctcatg atcgaccaga acatcatctt ttgcccggcg ttgtacttac cgacatccgc
    19501 cactttatgc tcattgcctt tcaatacttc gacaatgttc aacagccacg gaatatcttt
    19561 cttatccggg atgttgtgat gcacaaaacg cacaaacata aacatcagtg cgacgaaaat
    19621 cgcaatgccg aagaacgggt gcaaaatgcg tcccatctgc ggcgtaccga aggtttgcgt
    19681 cagccattgc agcgtcggga agaagaacga aatcccggac agcgccacca ggaagaagca
    19741 aatcaccacg gtccagtgac aggcgcgatc aataaatttg gtgcgcacaa tcattttcga
    19801 cttactcatg atgatcctcc tcgtcatcgt ccacttcctt attcgggcca ataccgatgt
    19861 agtggaaaat caacccggca aaagtggcaa taaagccagc cgctgccagc ggtttcaacg
    19921 cgcctttcca caggcttacc gaggtgtcga tcttcggatc tttcggcaga ccgtgataca
    19981 gctccggctg atcggcgtga tgcagcacgt acataacgtg cgtaccaccg accccttccg
    20041 ggttgtagac gccagcatgt tcgtaaccac gcgctttcag tttcgccacg cgctgttccg
    20101 ccagctccag catctccttc ttggtgccga agtggatagc cccggtcgga caggttttca
    20161 cacaagccgg ttcctggccg acgctgacgc gatcgacgca gagcgtgcat ttatataccc
    20221 ggttatcctc tttgttgagg cgcggaatat taaacggaca cccggcaatg cagtaaccac
    20281 agccgatgca gttttccgac tggaaatcga caatcccgtt agcgtactga atgattgcac
    20341 cagcagacgg gcacgcctta aggcagcccg gatcttcaca gtgcatacag ccgtctttac
    20401 ggatcagcca ctccagcttg ccgttctgtt cggtttcgct aaagcgcatc accgtccagg
    20461 acttggcgct cagatcggcg gggttatcgt aaaccccgac gcagtgcccc acttcatcac
    20521 ggatgtcgtt ccactccgaa cacgccacct gacaggcttt acaaccgata caggtggaaa
    20581 cgtcgataag ttttgcgact tctgctttgt aatcacgcac ctgagaaggc ggcgtgatgg
    20641 agttagttgc ggaccttttg ataatgtcct gcgtttccat agccatctgt tcgccccctt
    20701 acgccttctc gatgttgact aagaacgctt tatattccgg cgtttgcgag tttgcatcac
    20761 cgacattcgg cgtcagagtg ttagcgatat aacctttacg cgcgacaccc tcaaagcccc
    20821 agtggattgg aatacccacc gtttcaacct gctgaccgtt tacattcagc ggcttcagac
    20881 ggcgcgttac cactgccacc gcgcggataa agccacgttt gcttgagaca gtgacacgat
    20941 cgccattatt aatgcctttc gccgccgcca gcgtttcgct gatttccaca aactgttccg
    21001 gctgagcaat tgcgttgagc aatgcgtgct tggtccaggt gtggaagtgc tcggtcagac
    21061 gataggtcgt acccacatac gggaactgct cttttttacc catccgcagc gcgtcttgtt
    21121 catacagacg aacaaccggg ttagacacca cgttcggatg cagcgggtta gtgcccagcg
    21181 gcgtttcaat cggctcgtag tgttccggga acggaccttc cgccattttg ttgatggcaa
    21241 acaggcgtcc catcccttcc ggctgcatga taaacggccc ggttggcgta cccggtgcgg
    21301 cattgccgaa gtcaggaata tcgttacccg tccacttgct gccgttccac tggatcagca
    21361 tccgtttcgg atcccacggt ttaccgttga tatccgccga agcacggttg tacagcacgc
    21421 gacggttgag cggccacgcc caggcccatc caagcgtatt ccccagaccg gacgggtcgg
    21481 agttatcgcg gttagccatc tggttgccct gctctgtcca gctaccggtg tagatccagc
    21541 aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa gctactcagc aactgacctt
    21601 tcttcgcaat cagcacgcca tttgcgtcat agagatcttc cagcgcatag ccgttgttct
    21661 cttttgccac ttcgtcagat tgcggttcgt gcggctgctt gtagttccag ctcatcttca
    21721 tcagcggttc tacgccttta ccaccttcgg cctggtacag ctcgcgcaga tgatggtaga
    21781 tacccgccag aatttcaccg tcgttacgcg cttcgcccgg cgcgtcctga cctttccagt
    21841 gccactgcaa ccagcgaccg gagttagcga tagaaccatc ttcttcagca aagcaggtcg
    21901 aaggcagacg gaatacttca gtctgaatag acgccggatc gacatcgttc gactcaccgt
    21961 ggttctgcca gaaggtagag gtttcagtca ccagcggatc gataaccacc atgtacttca
    22021 gcttgctcag gcagctcacc actttgtttt tgtccgggaa ggacgcaacc gggttaaagc
    22081 cctggcagaa ataaccggtg actttgcctt catccatcat gttgaaatac ttgatgacgt
    22141 cgtaggtctg gtcccacttc ggcagccagt catagcccca gttgttctct ttctgcgcgg
    22201 catcgccata gaaagatttc atcaggctaa cgaagaactt cggatagttg ctccagtagt
    22261 tcacctgatc agccagcgtc gctttcggcg tgttcgcttc cagatacgac tgcaaatcaa
    22321 cctgtttttc tgacggcagc gtcagataac ctggcaggct ggtagagagc aggcctaagt
    22381 cagtcaagcc ctgaatgttg gagtgaccac gcaatgcgtt cacgccgcca ccggccatac
    22441 ccatgttacc gagcagcaac tggatcatcg ccatagtacg gatgttctgc gcacccacag
    22501 tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt gcgatccggt gcgctggtgg
    22561 aggccagcac ttcacacact ttcaggaagt cggcttttgg cgtaccgcag atgttttcaa
    22621 cgacgtccgg cgtgtagcgg gaaacgtgct ctttcagcag gttccacaca cagcgcggat
    22681 gagtcagtgt ttcatcgcgt ttcgcatagc cgttttcatc gaactgatag ttccaggacg
    22741 atttatcgta ttgacgtttt tcagcgtcgt agccgctgaa cagaccgtct tcgaaagcaa
    22801 aatcatcacg caccagcagg ctggcgttgg tgtaatgctt aacgtattcg gcgttgattt
    22861 tgttgttttc gatcaggtag cgcaaaacgc cagacaggaa cgtaatgtcc gtaccggaac
    22921 gaataggcgt gtaaatatcc gccacagaag cggtacgcgt aaaacgggga tcgacaacga
    22981 tcaaggttgc gtcgttgttg tttttcgctt ccatcgccca gcggaaaccg acgggatgcg
    23041 cttcagcagc gttaccgccc atcaccatca cgacgttagc gtttttgata tccacccagt
    23101 ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc
    23161 agacgcgcgc ctggttgtct accgccagca tcccgaggga gcgggcaaat ttctgggtca
    23221 gcatcccggt ttcgttgctg gcaccggagg cacacagcat accggtagaa agccaacggt
    23281 ttaccgttac gccctgctcg ttcttttcaa taaagttagc gtcacggtca gctttcatca
    23341 gcttcgcaat acgggagaat gcttcttccc agctaatgcg ctgccatttg tcagaacctg
    23401 gcgcacgata ttccgggtag cgcagacggt tttcactgtt gacgtaatcc agcaaaccgg
    23461 cccctttcgg gcacagcgca ccacggctta ccggatgatc cgggtcacct tcaatgtgat
    23521 aaatcgcttc tctggcgttt tttgcgccat cacccaggct atacatcaat agcccgcaac
    23581 ctacggaaca gtatgtgcag gtgttacgga tctctttagc gcgtaataat ttgtagtttc
    23641 gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc cgctactgtt gttccagcca
    23701 taccgcccgc gcagatttta aaaaattgtc tgcgactgac gtccattgct ttcctctttt
    23761 ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt ctgaatcttt ttgcgcgaaa
    23821 tcaaaaagaa catctgtttg ccacaaaata atccgccttc gcaaattgac ctacctcaat
    23881 agcggtagaa aaacgcacca ctgcctgaca ggccagttaa aaaaatgcta taaaattcag
    23941 cttaattttt aacggcaaga gagacaaaac agcgagcatg acacgacaaa aagcaacgct
    24001 catagggctg atagcgatcg tcctgtggag cacgatggta ggattgattc gcggtgtcag
    24061 tgaggggctc ggcccggtcg gcggcgcagc tgctatctat tcattaagcg ggctgctgtt
    24121 aatcttcacg gttggatttc cgcgtattcg gcaaatcccg aaaggctatt tactcgccgg
    24181 gagtctgtta ttcgtcagct atgaaatctg tctggcgctt tccttagggt atgcggcgac
    24241 ccatcatcag gcgattgaag tgggtatggt gaactatctg tggcccagcc tgacaattct
    24301 ctttgccatt ctgtttaatg gtcagaaaac caactggttg attgtacctg gattattatt
    24361 agccctcgtc ggcgtctgtt gggtgttagg cggtgacaat gggttacatt atgatgaaat
    24421 catcaataat atcaccacca gcccattgag ttatttcctg gcgttcattg gtgcgtttat
    24481 ctgggcagcc tattgcacag taacgaataa atacgcacgc ggatttaatg gaattaccgt
    24541 ttttgtcctg ctaacgggag caagtctgtg ggtttactat tttcttacgc cacaaccaga
    24601 aatgatattt agcacgcccg tcatgattaa actcatctct gcggcattta ccttaggatt
    24661 tgcttatgct gcatggaatg tcggtatatt gcatggcaat gtcaccatta tggcggtagg
    24721 ttcgtatttt acgcctgtac tttcctcagc gcttgcagcc gtgctgctca gcgccccgct
    24781 gtcgttctcg ttctggcaag gcgcgctgat ggtctgcggc ggttccctgc tctgctggct
    24841 ggcgacacgt cgtggttaaa ataactgccg ggtctacggc ccggtagtta atcagataaa
    24901 ttccgacaat attaaaaaaa taaatttcaa ataccagtaa ttcacctta
//
LOCUS       DAZFZC010000078        24831 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFZC010000078 DAZFZC010000000
VERSION     DAZFZC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.078"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            151..1356
                     /gene="yedE"
                     /locus_tag="ACHKXP_003487"
     CDS             151..1356
                     /gene="yedE"
                     /locus_tag="ACHKXP_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO7648430.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     SKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1353..1586
                     /gene="yedF"
                     /locus_tag="ACHKXP_003488"
     CDS             1353..1586
                     /gene="yedF"
                     /locus_tag="ACHKXP_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO7648431.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1695..2363
                     /gene="yedK"
                     /locus_tag="ACHKXP_003489"
     CDS             1695..2363
                     /gene="yedK"
                     /locus_tag="ACHKXP_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO7648432.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQHV"
     gene            complement(2441..3580)
                     /locus_tag="ACHKXP_003490"
     CDS             complement(2441..3580)
                     /locus_tag="ACHKXP_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5503 domain-containing protein"
                     /protein_id="HFO7648433.1"
                     /translation="MFPLNDLSLKTQSVQLNKVTSNTESTIKQQELVSDDAMINELSS
                     ELVSCLGNGKFTPISEGSKLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKMGSITI
                     EKSQGEGTLPIRHKLEFISTNIAELLGKLTKITDARLCKGFSDWASSVKEGASNDFKE
                     NVDRALVRLFKCVELHSNELNLSYLFLGSVPPLPEWIEMLSLIHNELDSIHVPESCKE
                     LEVDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKAKIISICHNKLSEIPTIPDTA
                     KVFDCSENNIKEIRWFPKNLKEVHIEYNKIEVIPAMPGNLKLLFMECNPIKEAFLMPW
                     TLTGICYEISQRKYIVTNPDDYDKYSDMVKKYVIDGEDHLIKYYM"
     gene            3764..3904
                     /locus_tag="ACHKXP_003491"
                     /pseudo
     CDS             3764..3904
                     /locus_tag="ACHKXP_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3929..4243)
                     /gene="fliE"
                     /locus_tag="ACHKXP_003492"
     CDS             complement(3929..4243)
                     /gene="fliE"
                     /locus_tag="ACHKXP_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO7648434.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLQQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4458..6116
                     /gene="fliF"
                     /locus_tag="ACHKXP_003493"
     CDS             4458..6116
                     /gene="fliF"
                     /locus_tag="ACHKXP_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO7648435.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            6109..7104
                     /gene="fliG"
                     /locus_tag="ACHKXP_003494"
     CDS             6109..7104
                     /gene="fliG"
                     /locus_tag="ACHKXP_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO7648436.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLVNRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7097..7783
                     /gene="fliH"
                     /locus_tag="ACHKXP_003495"
     CDS             7097..7783
                     /gene="fliH"
                     /locus_tag="ACHKXP_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO7648437.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7783..9156
                     /gene="fliI"
                     /locus_tag="ACHKXP_003496"
     CDS             7783..9156
                     /gene="fliI"
                     /locus_tag="ACHKXP_003496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO7648438.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9175..9618
                     /gene="fliJ"
                     /locus_tag="ACHKXP_003497"
     CDS             9175..9618
                     /gene="fliJ"
                     /locus_tag="ACHKXP_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO7648439.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            9615..10742
                     /gene="fliK"
                     /locus_tag="ACHKXP_003498"
     CDS             9615..10742
                     /gene="fliK"
                     /locus_tag="ACHKXP_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO7648440.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQVRVTGNSGVDIFA"
     gene            10847..11311
                     /gene="fliL"
                     /locus_tag="ACHKXP_003499"
     CDS             10847..11311
                     /gene="fliL"
                     /locus_tag="ACHKXP_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO7648441.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11316..12320
                     /gene="fliM"
                     /locus_tag="ACHKXP_003500"
     CDS             11316..12320
                     /gene="fliM"
                     /locus_tag="ACHKXP_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO7648442.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12317..12730
                     /gene="fliN"
                     /locus_tag="ACHKXP_003501"
     CDS             12317..12730
                     /gene="fliN"
                     /locus_tag="ACHKXP_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO7648443.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12733..13098
                     /gene="fliO"
                     /locus_tag="ACHKXP_003502"
     CDS             12733..13098
                     /gene="fliO"
                     /locus_tag="ACHKXP_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO7648444.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            13098..13835
                     /gene="fliP"
                     /locus_tag="ACHKXP_003503"
     CDS             13098..13835
                     /gene="fliP"
                     /locus_tag="ACHKXP_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO7648445.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13845..14114
                     /gene="fliQ"
                     /locus_tag="ACHKXP_003504"
     CDS             13845..14114
                     /gene="fliQ"
                     /locus_tag="ACHKXP_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO7648446.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14122..14907
                     /gene="fliR"
                     /locus_tag="ACHKXP_003505"
     CDS             14122..14907
                     /gene="fliR"
                     /locus_tag="ACHKXP_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO7648447.1"
                     /translation="MLQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            15197..15820
                     /gene="rcsA"
                     /locus_tag="ACHKXP_003506"
     CDS             15197..15820
                     /gene="rcsA"
                     /locus_tag="ACHKXP_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO7648448.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15864..16052)
                     /gene="dsrB"
                     /locus_tag="ACHKXP_003507"
     CDS             complement(15864..16052)
                     /gene="dsrB"
                     /locus_tag="ACHKXP_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO7648449.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16215..16442
                     /gene="yodD"
                     /locus_tag="ACHKXP_003508"
     CDS             16215..16442
                     /gene="yodD"
                     /locus_tag="ACHKXP_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO7648450.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16740..17555
                     /gene="yedP"
                     /locus_tag="ACHKXP_003509"
     CDS             16740..17555
                     /gene="yedP"
                     /locus_tag="ACHKXP_003509"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO7648451.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPAHVWRTQREGPEGWREGLDHFFSAC"
     gene            complement(17552..19246)
                     /gene="dgcQ"
                     /locus_tag="ACHKXP_003510"
                     /pseudo
     CDS             complement(17552..19246)
                     /gene="dgcQ"
                     /locus_tag="ACHKXP_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(19417..19599)
                     /gene="yodC"
                     /locus_tag="ACHKXP_003511"
     CDS             complement(19417..19599)
                     /gene="yodC"
                     /locus_tag="ACHKXP_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO7648452.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(19678..20595)
                     /gene="yedI"
                     /locus_tag="ACHKXP_003512"
     CDS             complement(19678..20595)
                     /gene="yedI"
                     /locus_tag="ACHKXP_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO7648453.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            20768..21688
                     /gene="yedA"
                     /locus_tag="ACHKXP_003513"
     CDS             20768..21688
                     /gene="yedA"
                     /locus_tag="ACHKXP_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO7648454.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21677..22147)
                     /gene="vsr"
                     /locus_tag="ACHKXP_003514"
     CDS             complement(21677..22147)
                     /gene="vsr"
                     /locus_tag="ACHKXP_003514"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO7648455.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22128..23546)
                     /gene="dcm"
                     /locus_tag="ACHKXP_003515"
     CDS             complement(22128..23546)
                     /gene="dcm"
                     /locus_tag="ACHKXP_003515"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO7648456.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23613..24308)
                     /gene="yedJ"
                     /locus_tag="ACHKXP_003516"
     CDS             complement(23613..24308)
                     /gene="yedJ"
                     /locus_tag="ACHKXP_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO7648457.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(24348..24629)
                     /gene="drpB"
                     /locus_tag="ACHKXP_003517"
     CDS             complement(24348..24629)
                     /gene="drpB"
                     /locus_tag="ACHKXP_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO7648458.1"
                     /translation="MEERLSRSPGGKPALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         6030 a         6473 c         6291 g         6037 t
ORIGIN      
        1 attaaagccc tgatgtcgcc agcctttcca ggttcagaga gaaatttgat ctaactatta
       61 tttttgtgaa ttattacccc gaaaataaat ttgcatggtg tatgattcgc gactcttgtt
      121 tcaaaccttc tgagttcaga ggctacattc atgtcatggc agcaattcaa acacgcctgg
      181 ttgattaaat tctgggcgcc catccccgcg gtcatcgcgg cgggtattct ctccacttac
      241 tattttggca ttactggcac cttttgggct gtcacgggtg aatttacccg ttggggcggc
      301 cagctcctgc aactgttcgg cgtccatgct gaagagtggg gttactttaa aattatccat
      361 ctggaaggat cgccattaac ccgcatcgac gggatgatga tcctcggtat gtttggcggc
      421 tgctttgccg cagcgctgtg ggccaacaat gtcaaactgc gaatgccgcg cagccgtatc
      481 cgcattatgc aggccatcat tggcggcatt atcgccggtt ttggcgcgcg tctggcaatg
      541 ggctgtaacc tggcggcgtt ctttaccggt attcctcagt tctcgctgca tgcctggttc
      601 tttgccatcg ccactgccat tggttcatgg tttggcgcgc gctttaccct gctgcccatc
      661 ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct caccgctgac gcaaaaaccg
      721 gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct ttttcggcat gctgggctgg
      781 gcgctgctca cagcgatgaa ccaatccaaa ctggggctgg caatgctgtt tggcgtcggc
      841 tttggtttac tgattgaacg tgcacaaatc tgctttactt cggcgttccg cgatatgtgg
      901 atcaccggac gtacccatat ggcgaaagca atcattattg gtatggcggt gagtgccatc
      961 gggatcttca gttacgtaca gttaggcgtt gaacccaaaa tcatgtgggc gggaccaaac
     1021 gcggtaattg gtggtttact gtttggtttt ggcatcgtgc tggctggcgg ctgcgaaacc
     1081 ggctggatgt accgcgcggt agaaggccag gtgcactact ggtgggtcgg tctgggcaac
     1141 gtgatcggct caacgattct ggcgtattac tgggatgatt tcgctccggc gctggccacc
     1201 gactgggaca aaatcaatct gctgaaaacc tttggtccga tgggtggcct gctggtgaca
     1261 tatttgctgt tgtttgctgc gctgatgttg attatcggct gggaaaaacg cttcttccgc
     1321 cgtgcggcac cgcagactgc taaggagatc gcatgaaaaa tatcgttcct gattaccgcc
     1381 ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc aacccttgag gcgatgccgc
     1441 agttaaaaaa aggggaaatc ctggaagtgg tgagcgactg tccgcagtcg atcaataata
     1501 ttccactgga tgcgcgtaat cacggctata cggtgctgga tattcagcaa gacgggccga
     1561 ccattcgtta tttaattcag aagtaatccc tcattcccgc tgtgacccac agcgggaaca
     1621 tcgttacctt tgtatcacat agattttacc cgccccctct cctattcttt tcagtatgat
     1681 ctcaggaggt ttctatgtgt ggacgctttg cccaatccca aacgcgtgaa gattaccttg
     1741 cgcttctcgc ggaagatatt gaacgcgata ttccctatga tcctgaaccc attggcagat
     1801 acaacgtcgc gccgggaacc aaagtccttc tgctcagtga acgtgatgaa caccttcatc
     1861 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgcca ctgattaacg
     1921 cccgcgtaga aactgccgca acgagtcgca tgtttaaacc gctctggcaa catggtcggg
     1981 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aagcagcctt
     2041 attttatcta tcgtgctgat ggacaacctg tttttatggc cgcgataggc agcacaccgt
     2101 tcgagcgcgg tgacgaagcc gaaggatttt tgattgtcac tgcagcagca gaccagggtc
     2161 tggtagatat tcatgaccgc cgcccactgg tactgtcgcc agaagctgca cgggaatgga
     2221 tgcggcaaga gattggcggt aaagaagcat cagaaattgc taccaatggc tgtgtgccag
     2281 ctaaccagtt cacctggcac ccggtatcgc gcgcggtggg taatgttaaa aaccagggcg
     2341 cggagttaat tcaacatgtt tgataagcct ggagattagt gatgaacaaa aatgtgtcac
     2401 aagaaacctc tattccagtg acacaattgc gcctaattac ttacatataa tatttaatta
     2461 ggtgatcctc accatctatt acatactttt taaccatatc ggaatattta tcataatcat
     2521 cggggttcgt aacaatatat tttcgctgcg atatttcata gcaaatccct gtaagagtcc
     2581 atggcattaa aaatgcctct ttaataggat tacactccat gaaaagtaat tttaaattgc
     2641 caggcatcgc aggaataacc tcaatcttat tatattcaat atgcacttct ttcagatttt
     2701 tggggaacca tctaatttcc tttatattat tctcactaca atcaaaaacc ttagcggtgt
     2761 ctggtattgt tggtatttct gatagcttat tatgacaaat agaaattatc ttagcctttg
     2821 gcggaaatga gtcaatatgc gatatcaggt tattatttac ggagatcagg gtaattccat
     2881 caggtacttg tggaaattct gtaaggttat tgaaatcgac ttctaattct ttgcatgatt
     2941 cgggcacatg tattgaatca agttcattat gaataaggct aagcatttca atccactcgg
     3001 gaagaggcgg cacagaaccc aagaaaagat atgataagtt aagttcatta ctgtggagct
     3061 caacacattt aaacaatctc accaatgctc tatccacatt ttctttgaag tcattggatg
     3121 cgccttcttt gactgaacta gcccagtcac tgaaaccttt gcaaagtctg gcatctgtaa
     3181 ttttggttaa cttgcccagc aactctgcaa tattggtcga tataaactct aatttatgtc
     3241 gaatgggtaa agtcccctcc ccctgacttt tttctatcgt gatagacccc atcttatcat
     3301 aagccccata attttcaaat aatagagtat aattacccca cctgaaacat tgctctctca
     3361 ataacttaaa ttctgacagc atattcaata atttgctgcc ttcactaatt ggtgtaaact
     3421 taccatttcc aagacaactg actaactcac ttgataattc atttatcatt gcatcatcag
     3481 atactaactc ttgttgtttt atcgtagact cagtattaga tgtgacttta tttaattgaa
     3541 ccgactgagt ttttaacgaa agatcattta atggaaacat ttacactcct gtttaataaa
     3601 ttatattgcc cagggagcat tctaaaacac aatgaaatta ccactatcaa aaatcgctca
     3661 tatccatagc ccgggatatg aataaaaaca tcaacaaaga ttaaattatt caaacgttac
     3721 tgttgcgtct gtggtcgtaa caacatccga cgtggtgatg tgtcaaatga gacactctga
     3781 tgatcagagg gtttatcgcg tatacggatc gtggatggca gaaaacgttc aggatcaggt
     3841 atccatttta aaccagaaaa tgagtgagtt tgccccatcc atgccccacg cggtgagatc
     3901 ggatgtaatc aacaacaggt taaaaaacct acacctgcat gctcatcact tcctgatacg
     3961 ccgccaccag cttattacgc acctgaatcc ccatttgcat agaaactgag gctttttgca
     4021 tatcggtcat cacatcgttt aacgccacgc cgggttcacc gagagtgaat ttttctgcct
     4081 gcgtgcgggc ggctgtttgt gtatcgctta tgcgatcgag cgcggcgtgc agctgcccgg
     4141 caaaactaat ggtcggttgc tgcagtgatt cctgcgcacg cgcactcatc gccgtagcct
     4201 gtaactggct gataacccct tcaatcccct gtatcgctga cattttcatc tcccggataa
     4261 tttctggtag caaagcctac cagtaagtca ataagacaaa ggcgctaaat agcaacaaaa
     4321 aaacgggttt attggcggat agaaaaaaac gaaagcacaa ataatgggag cgtcaatttt
     4381 tcgagtttgc tgacccggga gtgagtcttg ttccactttg ccaataacgc cgtccataat
     4441 cagccacgag gtgcgcgatg aatgcgactg cagcccagac aaaatctctt gagtggctta
     4501 atcgcctgcg tgcgaatccg aaaattccat tgattgttgc cggttccgcg gcagtggcgg
     4561 tcatggtcgc actgatcctg tgggcgaaag cccccgacta ccgcacatta ttcagcaatc
     4621 tttccgatca ggatggtggc gcaatcgtca gccaactgac gcaaatgaat attccttacc
     4681 gcttcagcga agccagcggc gctattgaag ttccggcaga taaagttcac gagctgcgtc
     4741 tgcgcctggc gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa ctgctcgatc
     4801 aggaaaagtt tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg gcgctggaag
     4861 gcgagctttc tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc gtacatctgg
     4921 caatgccgaa accgtcttta ttcgtccgtg aacaaaaatc cccttctgca tcggtgacgg
     4981 taaatctgtt acccggccgc gcactcgatg aagggcaaat tagcgccatt gtgcatctgg
     5041 tttccagcgc ggtggctggt ctgccgccgg ggaacgtcac gctggtggat cagggcggac
     5101 atctgttaac ccagtccaat accagcgggc gcgatcttaa tgacgctcaa ttgaaatatg
     5161 ccagcgatgt cgaaggccgt attcagcggc gtattgaagc gatcctgtcg cctattgttg
     5221 gtaacggtaa tattcacgcc caggtcacgg cgcaactgga cttcgccagt aaagaacaaa
     5281 cagaagaaca gtatcgccct aacggtgatg aatctcatgc ggcgcttcgt tcacgccagc
     5341 ttaatgagag cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg gcgttgtcga
     5401 atcaaccggc acctgcgaat aacgcgccaa tcagcacgcc tccggcaaat caaaataacc
     5461 gccagcagca ggcgagcacc accagcaata gtgggccgcg tagcacacag cggaatgaaa
     5521 ccagtaacta cgaagtcgat cgcaccattc gtcataccaa aatgaacgtg ggcgatgtgc
     5581 aacgtctgtc agtcgcggtt gtggtgaatt acaaaacctt gccagatggc aaaccgttgc
     5641 ctctcagcaa cgaacagatg aagcaaattg aagatctgac ccgcgaggcg atgggctttt
     5701 ctgaaaaacg cggtgactcg ctcaatgtcg tcaactcgcc gttcaatagc agtgacgaaa
     5761 gcggcggaga actgccgttc tggcaacagc aagcgtttat cgatcagtta cttgctgccg
     5821 gtcgctggtt gctggtactg ctagtggcgt ggctgctgtg gcgtaaagcg gtacgcccgc
     5881 agctaacacg tcgcgctgag gcgatgaaag ctgtacagca acaggcgcag gcccgcgagg
     5941 aagtggaaga tgcggtggaa gtccgcctga gcaaagacga acaactccaa caacggcgcg
     6001 ctaaccaacg tctgggggca gaagtcatga gccagcgtat ccgtgaaatg tctgataacg
     6061 atccgcgcgt ggtggcgctg gtcattcgcc agtggataaa taacgatcat gagtaacctg
     6121 acaggtaccg ataaaagcgt catcctgctg atgaccattg gcgaagaccg ggcggcagag
     6181 gtgttcaagc acctctccca gcgcgaggtg caaaccctga gcgctgcaat ggcgaacgtc
     6241 acgcagatct ctaacaagca gctaaccgat gtgctggcgg agtttgagca agaagctgaa
     6301 cagtttgccg cactgaatat caacgccaac gattatctgc gttcggtatt ggtgaaagcc
     6361 cttggcgaag agcgtgccgc cagtctgctg gaagatattc tcgaaacccg cgataccgcc
     6421 agcggtattg aaacgctcaa ctttatggag ccgcagagcg ccgccgatct gattcgcgat
     6481 gagcatccgc aaattatcgc caccattctg gtgcatctga agcgcgccca ggccgccgat
     6541 attctggcgt tgttcgatga acgtctgcgc cacgacgtga tgttgcgtat cgctaccttt
     6601 ggcggcgtgc agcctgccgc gctggcggag ctgaccgaag tactgaatgg cttgctcgac
     6661 ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa cggctgccga aattatcaac
     6721 ctgatgaaaa ctcagcagga agaagccgtt attaccgccg tgcgtgaatt cgacggcgag
     6781 ctggcgcaga aaatcatcga cgagatgttc ctgttcgaga atctggtgga tgtcgacgat
     6841 cgcagcattc agcgtctgtt gcaggaagtg gattccgaat cgctgttgat cgcgctaaaa
     6901 ggggccgagc agccactgcg cgagaaattc ctgcgcaata tgtcgcagcg tgccgccgat
     6961 attctgcgcg acgatctcgt caaccgtggt ccggtgcgtc tgtcgcaggt ggaaaacgaa
     7021 cagaaagcga ttctgctgat tgtgcgccgc cttgccgaaa ctggcgagat ggtaattggc
     7081 agcggcgagg atacctatgt ctgataatct gccctggaaa acctggacgc cggacgatct
     7141 cgcgccacca caggcagagt ttgtgcccat ggtcgagccg gaagaaacca tcattgaaga
     7201 gaccgaaccc agccttgagc agcaactggc gcaactgcaa atgcaggccc atgagcaagg
     7261 ttatcaggcg gggattgccg aaggtcgcca gcaaggtcat gagcagggct atcaggaagg
     7321 actggcccgg gggctggaac aaggtctggc agaggcgaag tctcaacaag cgccaattca
     7381 tgcccggatg cagcaactgg tcagcgaatt tcaaactacc cttgatgcgc ttgatagtgt
     7441 gattgcttcg cgcctgatgc agatggcgct ggaggcggca cgtcaggtga tcggtcagac
     7501 gccaacggtg gataactcgg cactgatcaa acagatccaa cagctgttgc agcaagaacc
     7561 gttattcagc ggtaaaccac agctgcgcgt gcatccggat gatctgcaac gtgtggatga
     7621 tatgctcggc gcaaccttaa gtttgcatgg ctggcgcttg cggggcgatc ccaccctcca
     7681 tcctggcggc tgtaaagtct ccgccgatga aggcgatctc gacgccagtg tcgccacccg
     7741 ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg taatgaccac gcgcctgact
     7801 cgctggctaa ccacgctgga taactttgaa gccaaaatgg cgcagttgcc tgcggtacgt
     7861 cgctacgggc gattaactcg cgctaccggg ctggtgctgg aagccaccgg attacaattg
     7921 ccgctcggcg caacctgtgt cattgagcgc cagaacggca gcgaaacgca cgaagtagaa
     7981 agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa tgccgctgga ggaagtcgaa
     8041 ggtgtcctgc ccggcgcgcg tgtatatgcc aaaaacattt cggcagaagg gctgcaaagc
     8101 ggcaaacagt tgccgctcgg tccggcgtta ttaggtcgcg ttctggacgg cagcggtaaa
     8161 cccctcgatg gcctgccctc ccccgatacg acggaaaccg gtgcgctgat taccccacca
     8221 tttaacccat tgcaacgtac accgattgaa catgtgctgg acaccggcgt gcgcccaatc
     8281 aatgccctgc ttaccgtcgg gcgcgggcag cgtatgggtc tgtttgctgg ttctggtgtc
     8341 ggtaaaagtg tgctgctggg gatgatggcc cgttacaccc gcgccgatgt cattgtcgtg
     8401 ggtttgattg gtgaacgtgg gcgcgaagta aaagatttca ttgagaatat cctcggtgct
     8461 gaagggcgtg cacgctcagt ggtgattgcc gctccggcgg atgtgtctcc gctcctgcga
     8521 atgcaaggtg ccgcctatgc cacgcgcatt gccgaagatt ttcgcgatcg tggtcagcat
     8581 gtgttgctga ttatggactc cctcacccgc tacgcgatgg cccagcgtga aattgcgctg
     8641 gcgattggcg aaccacccgc caccaaaggt tatccaccgt cggtgtttgc caaattaccg
     8701 gcactggtcg agcgtgccgg aaatggcatt agcggcggcg gctcgattac tgcgttttat
     8761 accgtgctca ccgagggcga tgaccagcag gatccgattg ccgactccgc gcgggcaatc
     8821 ctcgacggcc acattgtgct gtctcgccga ctggcggaag ccgggcacta tccggctatc
     8881 gatattgaag cgtcgattag tcgtgcaatg acggcgttga tcagtgagca acattacgcg
     8941 cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc gtaaccgcga tctggttagc
     9001 gtcggcgcgt atgccaaagg cagcgatccg atgctcgata aagccatcgc cctgtggccg
     9061 cagctggagg gctatttgca acaaggcatt tttgaacgcg cggactggga agcgtctctc
     9121 caggggctgg agcgtatttt cccgacagtg tcataaccca ggagataacg gcagatggca
     9181 gaacatggtg cgctggcgac cctgaaagat ctggcagaaa aagaggtaga ggatgccgcg
     9241 cgcctgctgg gtgaaatgcg tcgcggatgt cagcaggcgg aagaacagct caaaatgctg
     9301 atcgattatc agaatgaata tcgcaataac ctcaacagcg atatgagtgc cgggatgacc
     9361 agcaaccgct ggatcaacta tcagcagttt atccagacgc tggaaaaagc cattactcag
     9421 catcgccagc aacttaatca gtggacgcag aaagttgaca ttgccctgaa cagctggcga
     9481 gaaaagaaac aacgtttgca ggcctggcag acactgcagg aacggcaatc cacggcggca
     9541 ctgcttgcag aaaaccgcct cgatcagaaa aagatggatg agttcgccca gcgcgccgcc
     9601 atgaggaaac ctgaatgatt cgcttagcgc ccttaattac cgccgacgtt gacaccacca
     9661 cattgcctgg cggcaaagcc agcgatgctg cacaagattt tctcgcgttg ttgagcgaag
     9721 cattagcagg cgagacaact accgacaaag cggcccccca gttgctggtg gcaacagata
     9781 agcccacgac aaaaggcgag ccgctgatca gcgatattct tgccgacgcg caacaagcgg
     9841 atttactgat ccccgtggat gaaacaccgc ctgtcatcaa cgacgaacaa tccacatcaa
     9901 caccattaac caccgctcaa acgatgacgt tggctgcggt ggctggcaac aatacggcaa
     9961 aagacgaaaa agcggatgat ctgaatgaag acgtcaccgc aagcctgagc gccctttttg
    10021 cgatgttgcc gggttttgac aatacgccca aagtgactga tgtgccgtca accgtgttac
    10081 cggcagagaa accaacgctc ttcacaaaac tgacttctgc gcaactcaca acagcacagc
    10141 ctgatgatgc ccccggcacg ccagctcagc cattaacacc gctggtagca gaagcccaga
    10201 gtaaagcgga agtcatcagc acaccttcac cggtgaccgc tgccgccagc ccgctaatca
    10261 ctccacacca gacacagcca ctgcccaccg tcgccgcgcc tgtgttgagt gcaccgctgg
    10321 gttctcacga atggcaacaa tcattaagcc agcatatttc gctgttcacc cgccaggggc
    10381 aacaaagtgc agagttgcgt ctgcacccgc aggatttagg tgaagtgcaa atctccctca
    10441 aagtggatga taaccaggcg caaatccaga tggtttcacc gcatcaacac gtacgcgccg
    10501 ccctggaagc agcgcttccg gtactgcgaa cgcagctggc cgaaagtggc attcagttag
    10561 ggcaaagcaa catcagtggc gaaagcttta gtggtcagca gcaggccgct tcccagcaac
    10621 agcaaagcca acgcacagca aaccatgaac ctctggcggg ggaagacgac gatacgcttc
    10681 cggttcccgt ctctttacaa gtacgtgtaa caggcaacag cggcgttgat attttcgcct
    10741 aacgtcagag gtagcaccgt aatccgcgtc ttttccccgc tttgttgcgc tcaagacgca
    10801 ggataattag ccgataagca gtagcgacac aggaagaccg caacacatga ctgattacgc
    10861 gataagcaag aaaagcaagc gatcgctttg gatcccgatt ctggtattca ttaccctcgc
    10921 ggcctgtgcc agcgcaggtt acagctactg gcattcgcat caggttgccg ctgacgacaa
    10981 agcgcagcaa cgcgtcgtgc cctccccggt cttctatgcg ctggatacct ttacggtcaa
    11041 tctgggcgat gcagatcgcg tactttatat cggcataacc ctgcgcctga aagatgaagc
    11101 tacccgctcg cggctgagtg agtatttgcc ggaagtccgt agccgtttgc tgttactgtt
    11161 ttcgcgtcag gatgctgccg tactggcgac agaagaaggc aagaaaaacc tgattgccga
    11221 gattaaaacc acgctttcca ccccgcttgt tgccgggcaa ccgaaacagg atgtcaccga
    11281 cgtgctgtat accgctttta ttctgcgata acgacatggg cgatagtatt ctttctcaag
    11341 ctgaaattga tgcgctgttg aatggtgaca gcgaagtcaa agacgaaccg acagccagtg
    11401 ttagcggcga aagtgacatt cgtccgtacg atccgaatac ccaacgacgg gttgtgcgcg
    11461 aacgtttgca ggcgctggaa atcattaatg agcgctttgc ccgccatttt cgtatggggc
    11521 tgttcaacct gctgcgtcgt agcccggata taaccgtcgg ggccatccgc attcagccgt
    11581 accatgaatt tgcccgcaac ctgccggtgc cgaccaacct gaaccttatc catctgaaac
    11641 cgctgcgcgg cacagggctg gtggtgtttt caccgagtct ggtgtttatc gccgtggata
    11701 acctgtttgg cggcgatgga cgcttcccga ccaaagtgga aggtcgcgag tttacccata
    11761 ccgaacagcg cgtcatcaac cgcatgttaa agctggcgct tgaaggctat agcgacgcct
    11821 ggaaggcgat taatccgctg gaagttgagt acgtgcgttc ggaaatgcag gtgaaattta
    11881 ccaatatcac cacttcgccg aacgacattg tggttaacac gccgttccat gtggagattg
    11941 gcaacctgac cggcgaattt aatatctgtc tgccattcag catgatcgag ccgctacggg
    12001 aattgttggt taacccgccg ctggaaaact cgcgtaatga agatcagaac tggcgcgata
    12061 acctggtgcg ccaggtgcag cattcacagc tggagctggt cgccaacttt gccgatatct
    12121 cgctacgcct gtcgcagatt ttaaaactga aacccggcga cgtcctgccg atagaaaaac
    12181 ccgatcgcat catcgcccat gttgacggcg tcccggtgct gaccagtcag tacggcactc
    12241 tcaacggtca gtatgcgtta cggatagaac atttgattaa cccgatttta aattctctga
    12301 acgaggaaca gcccaaatga gtgacatgaa taatccggcc gatgacaaca acggcgcaat
    12361 ggacgatctg tgggctgaag cgttgagcga acaaaaatca accagcagca aaagcgctgc
    12421 cgacgcggtg ttccagcaat ttggcggtgg tgatgtcagc ggaacgttgc aggatatcga
    12481 cctgataatg gatattccgg tcaagctgac cgtcgagctg ggccgtacgc ggatgaccat
    12541 caaagagctg ttgcgtctga cgcaagggtc cgtcgtggcg ctggacggtc tggcgggcga
    12601 accactggat attctgatca acggttattt aatcgcccag ggcgaagtgg tggtcgttgc
    12661 cgataaatat ggcgtgcgga tcaccgatat cattactccg tctgagcgaa tgcgtcgcct
    12721 gagccgttag tgatgaataa ccacgctact gtgcaatctt ccacgccggt ttctgctgcg
    12781 ccactgctgc aggtcagcgg cgcactcatc gccattattg ccctgatcct cgctgctgcc
    12841 tggctggtaa aacggttggg atttgccccg aaacgcactg gcgttaacgg tctgaaaatt
    12901 agcgccagtg cttcgctggg cgcgcgtgaa cgggttgtgg tggtcgatgt ggaagatgca
    12961 cggctggtgc tcggcgttac cgcagggcaa atcaatctgc tgcataaact tcccccttca
    13021 gcaccaacag aagagatacc gcagaccgat tttcagtcgg tcatgaaaaa tttgcttaag
    13081 cgtagtggga gatcctgatg cgtcgtttgt tgtctgtcgc acctgtcctt ctctggctgg
    13141 ttacgcccct cgccttcgcg caactgccgg gtatcaccag ccagccgctg cctggcggtg
    13201 gacaaagctg gtcgctcccg gtgcagacgc tggtgttcat cacctcgttg acgtttattc
    13261 cggcaatttt actgatgatg accagtttca cccgcatcat cattgttttt ggtttattgc
    13321 gtaacgcgct gggaacgccc tccgcgccac ctaaccaggt attgctgggg ctggcactat
    13381 ttttgacctt ttttattatg tcaccggtga tcgacaaaat ttatgtcgat gcgtaccagc
    13441 cattcagcga agagaaaata tcaatgcagg aggcgctgga aaaaggggcg cagccgctac
    13501 gtgagtttat gctgcgtcag acccgtgagg cagatttagg gttgtttgcc agactagcga
    13561 ataccggccc gttgcaggga cctgaagccg tgccgatgcg cattttgctc ccggcctacg
    13621 tgaccagcga gttgaaaacc gcatttcaga taggcttcac gattttcatc ccttttttga
    13681 ttatcgacct ggtgatagcc agcgtgttga tggcattagg gatgatgatg gttccccccg
    13741 ccaccattgc tctgcccttt aaactgatgc tgtttgtact ggtggatggc tggcaattgc
    13801 tggtcggttc gctggcgcag agcttttaca gctagagagg caaaatgaca cctgaatcgg
    13861 tcatgatgat ggggactgaa gcgatgaaag tcgcgctggc actggctgcc ccgctattgt
    13921 tggtagcgtt ggtcacgggc cttatcatca gtattttgca ggccgccacc cagattaacg
    13981 aaatgacgct gtcgtttatt ccgaaaatca tcgccgtatt tattgccatt attattgccg
    14041 gaccgtggat gctcaatctg ttgctggatt acgtccgcac cttgttcact aacctgccgt
    14101 atatcatcgg gtagccgtac tatgttgcag gtgacaagcg atcaatggct ttcctggcta
    14161 agtctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc gccaattctg
    14221 agcgaacgca gcgtgccgaa gcgggtaaaa ctgggtctgg caatgatgat cacgttcgct
    14281 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    14341 gccgtgcagc agatcctgat cggcattgcg cttggtttca ccatgcaatt tgcctttgcc
    14401 gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt
    14461 gtcgatccgg ccagccatct caacatgccc gttctagcgc gtatcatgga tatgctggcg
    14521 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc
    14581 tttcacaccc tgccgattgg tggcgaaccg ttgaacagca atgcgtttct ggcactcacc
    14641 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg
    14701 ctgctgaccc tgaatctggc attaggttta ctgaatcgta tggccccgca attatccatt
    14761 tttgttattg gatttccatt aactctgact gtcggtatct ctttaatggc ggcattaatg
    14821 ccgttaattg cgcctttttg cgaacattta ttcagtgaga tgtttaattt gctggctgat
    14881 attattagtg aattgccact aatataattc cgtaacgttt atcatgttat cctaaggatt
    14941 atccgaaaaa taatacctac gaacatcttc caggatactc ctgcggcgaa atatttgttt
    15001 taagctcact cacatatcgc aacatttact ttactttaag acaattccag gcaaattata
    15061 caacacttta cgggatagta agtccgcctg aaaaatcgcg agagtggcgc attaggtgac
    15121 ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat
    15181 tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg
    15241 tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac
    15301 cgttgatgac cttgccatag cttgtgattc acagcgccct tcagtggtgt ttattaatga
    15361 ggactgtttc atccatgatg cttctaacag tcagcacatc aagcacatca ttaatcaaca
    15421 tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct
    15481 attggtcaga aaaaatttat tgataagttc taaatcgatt aaaccggaat ctctcgacga
    15541 tatccttggc gatattctga aaaaagagac aacgataacc tcttttttaa atatgccgac
    15601 gttatcattg agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac
    15661 cattcaaatc tctgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa
    15721 tattaaacgt aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac
    15781 ggataatgtg actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt
    15841 cccaccagtc aggctgaata agattactct gctttctcca caaagatacc gtcctgatgc
    15901 cctgcttcat taaagaacca gatgccgagc gggtagtctt ccagcgaaac caggtacatt
    15961 gtgccttcac taaactcctc aactgccagt accacgccag gacgacgcgg accgccatcc
    16021 gttttgactg ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt
    16081 gtagaacaat ttcgtttttc tggcagcgcc aggcgcgcgc gagtgctgat tttctcgacg
    16141 gtctatactt aagagatgcc aggcggactt aacgactggc ggcaacaaca gagtaacggt
    16201 tgcgaggaaa gatgatgaaa accgcaaaag agtacagcga taccgcaaaa cgtgaggtca
    16261 gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc
    16321 gcagccagaa cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg
    16381 aattggcgga tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt
    16441 gaatgcacaa taaaaaaatc ccgaccctga gggggtcggg atgaaacttg cttaagcaag
    16501 aagcacttaa aaaattcgtt acaccaggaa atctgatgtg ttcatcacct tatccgcaat
    16561 ttttttcgct gacaagaaaa tattcgctat atgaatgatt aacgctactt atgaatagaa
    16621 atgtgacgca cgtcacccct tggtggctct ggcgtcagcg ggatcgctcc cgaaaaaatc
    16681 atctttgata atttgtgctg ttttgagaat catcctggta aggggagaac tgcgcgacca
    16741 tgttttcaat tcaacaacca ctactggttt ttagcgatct tgatggcacc ctgctggaca
    16801 gtcatagtta tgactggcaa ccagctgccc cctggctcag ccgtttacgc gaagcgaatg
    16861 ttcccgtcat tctctgtagc agtaaaactt cagcggaaat gctgtacttg caaaaaacgt
    16921 tggggctaca agggttaccg ctgattgccg aaaatggcgc agtgatccag cttgctgaac
    16981 aatggcagga tatagacggt tttccacgca tcatctcagg tattagccat ggcgaaatca
    17041 gccaggtttt aaatacgcta cgcgagaaag aacattttaa attcacaact tttgatgatg
    17101 tcgacgatgc aaccatcgcc gaatggacgg gattaagccg tagccaggcg gcgctgacgc
    17161 agctgcatga ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt atggcacaat
    17221 ttaccgctcg tctgaacgaa ctgggcttac agtttatgca gggtgcgcgc ttctggcacg
    17281 tcctggatgc ctctgccgga aaagatcagg ctgccaactg gattatcgcg acctatcaac
    17341 aattgtcagg caaacgccca accacacttg gcctgggcga tgggccaaac gatgcgccct
    17401 tactggaggt aatggattac gcggtgattg tgaaagggct aaaccgtgaa ggggtgcatc
    17461 tgcatgatga ggatccggcc cacgtctggc gaacgcagcg tgaaggaccg gaaggatggc
    17521 gtgaagggct ggaccatttt ttctccgcct gttaagcgtt atcgctcgcg aatacccgat
    17581 tacgcccagc ctgtttagcg agataaagcc gacggtcggc cagtgactgg agttgttcaa
    17641 aatcataatc accggtttcc tcgctgctac ttacccccaa cgaggcactg atgcgtatcg
    17701 tcgtactctt ggcaatcaac atctcttttt catttaactt caggcgaata cgttctgcga
    17761 cttccgcagc ctccgtcaga ctcgcgcctg gcagaatcac acaaaactcc tcaccaccga
    17821 cccgcccggc aacgtcctgc gcacgcaagg aactgctaat taatccggca gcatgagaaa
    17881 gaacacggtc gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat
    17941 cgacctgaat gacagaaaaa ggatgttggt gcgtctgaca caatttagcg agcggacggg
    18001 ctttttcgaa cagtgcgcca cgattatata aacgggttaa ggtgtcgtgc cacgcctgcc
    18061 actgcaacga gctttgcaga acatacatgt tgctgaccat ccggcgaatc acataccagg
    18121 agatgagtaa catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc
    18181 cgaaatcgcc gcgcacgcct tcgcttaacg tatggacgcg caccagcaca ccgtcgaaat
    18241 ggttcagacg ttcccagcta acatagcgac tgtccatacg aatgccgccc cgcgtgtcat
    18301 gttccattgc ctgcgccagc aaggccagtt cacgagggtc aaaaatattc cctgttggat
    18361 ggtcaggatt ggaagaggtc aaaaatctca gcttactgtc atagagctga tactcaccat
    18421 cgaggttttt atcgatggcg tttcttaaaa attgctgcat ggtacgcacg ggaatactca
    18481 tccccagcac gccataccag tagttattac tgtctaccgg aacactgacg gtaacctgcg
    18541 gttcagtatt gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat
    18601 tttctcgttg cgaatggccg ataaaccagg gttgggtgac atagccgtaa taacgcgttg
    18661 gtacattgcg cgtaaacaag gtcggctgcg tcgaaacgta aaatccggca cgcgagacat
    18721 acatcgccta ttcaaccatc gacgaggtgt tgtgcgccag tcgcagcaag taaccaactt
    18781 ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc gcgagacagg agattcccct
    18841 cgctgactaa tgcatccgat acgccattga ctgacagggt gcgtcgtcgg ttgagttcga
    18901 tttgccaggc gtgctcgtcg cgatgctgtt cgaactcggt tacagcatca cgcagagagg
    18961 tgaaatccag tggcgctacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt
    19021 cgacgttata ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct
    19081 ggctggagat ataggcatct tccagcacta ccacttcacg ccaggtcagc aaggtggaaa
    19141 aaagcaatac cacgataaag cagagattaa cgacatgacc aggccccagg cggcgtgcga
    19201 gttttttcaa ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactccca
    19261 acctgctttt ttatgattct ggcataacct ggcgatagcg taccgcaaaa cagcgataat
    19321 tttataatgg tggcatgata agacgcgact atcattcatt gcaggcattg gcacaccagg
    19381 ccggatgtga tccggcctgg tgttaatgat accctttcaa acttcttccg cagaacgact
    19441 cccctccccc ggaacaagct cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc
    19501 ataccaacga cactcaacca taccgctgga gtatccagtg acaatcatcc gcgggccgcc
    19561 ctctttaacc gtaacttcct cactaaccat aaagctcata ctcgcctcct tttttcgagt
    19621 gaaacttgtt caccttagtt gaagatggcg aattttgcat aatagcctgt gcgagtatta
    19681 atgtgcctga ccgcgtattt tcgctacggc tttcactccc agcaccacga tgccgccgat
    19741 gataaatcca agaatcagat ttaaaacagt cggtaatatc atcgccacca ctgcactttg
    19801 ctgcccggcg aaatgttcaa tggcgtgatg cagcggcgca ataccatgta ccacaatccc
    19861 gccgccgacg aggaacatcg ccagcgtgcc gacaatcgat aatgctttca tcaaccaggg
    19921 cgcaatgatc aataaccctt tacctaatgc ctgcatcagc gcgctggatt tttccgccag
    19981 ccaatacccc aggtcatcaa tcttaacgat aacccctacc agaccgtaga cgcccacggt
    20041 cactaccagc gcgatgcctg aaagcaccag cacctgatta agcaacggcg cttcggccac
    20101 aatccccagc gtgatggcga cgatttccgc agacaatata aaatcggtac gaatcgcccc
    20161 ttttattttg tccttttcaa acttcagcgg atcctgcgcc gccagcttct ccagacgctg
    20221 ctggctctgc gccggatctt ctttatgttt acgcgcctcc agcatatgca gcactttctc
    20281 tactccttca aagcagagaa acgcgccacc aatcatcaac agaggcgtaa tcgcccacgg
    20341 gataaacgca ctgatgatca gcgccagcgg caccagaatc actttattaa tcagcgatcc
    20401 tttcgccacg ccccagacca cgggaagttc ccggttggcc cgcacgcctg aaacttgttg
    20461 cgcattgagc gataagtcat cccctaatac accggcggtt ttcttcgccg ccagtttgcc
    20521 catcacggag atatcgtcca gcagtgtggc gatatcatcg agcaacgtca gtaaactact
    20581 cccggccaaa agcattccct ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa
    20641 cggcagctct gatacttgct tcacaggtca acattttttt aacaatcaag tgcaattata
    20701 tctctcgcca gcagaatgat tttcacgttt actatgaccc acctttttat ttccaccgtg
    20761 agggattatg cgtttccgcc agttgttacc gctttttggc gcgctgtttg cgttgtatat
    20821 catttggggc tcaacctatt ttgtcattcg gattggcgtg gaaagctggc ctccgttaat
    20881 gatggcaggc gtgcgattcc tggcagccgg tattttattg ctggcatttt tgctactgcg
    20941 cggacacaaa ctccccccgc tacgtccgct gcttaatgcc gcgctgattg gcctattatt
    21001 gctggctgtc ggtaatggca tggtgacggt tgccgaacat caaaatgttc cttccggcat
    21061 cgccgccgta gtggttgcaa ccgtgcccct ctttaccctg tgcttcagcc gcctgtttgg
    21121 cattaaaacg cgcaaactgg aatgggtggg tattgccatt gggcttgccg gaatcatcat
    21181 gctcaatagc ggtggaaatt taagcggcaa tccgtggggc gcgattctga ttttaatcgg
    21241 ctcgattagc tgggcgtttg gctcggttta tggctcgcgc attaccttac ctgtagggat
    21301 gatggcgggt gcgattgaga tgctggcggc aggcgtggtg ttaatgatcg cgtcgatgat
    21361 tgcaggtgaa aaactgacgg cgctcccttc cctttcaggc ttccttgcgg tcggctatct
    21421 ggcgctgttt ggttcgatta tcgccatcaa cgcttatatg tatttaatcc gtaatgtcag
    21481 tccggctctc gccaccagct acgcctacgt taacccggtg gtcgcggtct tgctgggtac
    21541 gggactgggc ggagagacgc tgtcgaagat tgaatggctg gcgctcggcg taattgtctt
    21601 cgcggtggta ctggtcacgt tgggaaaata tctcttcccg gcaaaacccg tagttgcgcc
    21661 agttattcag gacgcatcaa gcgagtaaat gaatcccctg cgtgtcgatc tgcgcgctgg
    21721 caccttcgcc gcagatccac tcttccagac gctcggtaag cgcttcatcc gtcagcttct
    21781 cgcgcccacg taatgcacac tcccagacaa tcagtacgcg ccagccgagt tcctgcaaac
    21841 gactgatatc gcggcgatcg cgttcaacat ttttacctat cttctccagc cagaactcgg
    21901 ttcgagtcgc aggcacttta aacagatagc agtgatgatg atgccagaag cagccatggg
    21961 taaatatcac gcagcgatat tcatcaacga caaaatccgg gcttccgggc agactggcgt
    22021 cctgaacgcg aaacgccagg ccctgcccgg ttaacagact ggcgaggcgc ttctcaatcg
    22081 ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg agtggcctta tcgtgaacgt
    22141 cggccatgtt gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt tggctcaagc
    22201 agttttgcca ccgcggcaaa gaccggcacg accaccgagt taccgaactg gcgataggcc
    22261 tgagtgtccg aaaccggaat gcggaatttc gcttctcccg gcgcttcaaa acccattaag
    22321 cgcgcgcatt cccgaggcgt taaccgacgt ggacgatgtt gctgattcag cggatcgtca
    22381 aaatctttct cacccgtggc catatcccag ccgcgatcga ttaaaatttc cgcgccatct
    22441 ttgtaataac gcgcagacag cgtacgcgtg acgctttgcg gattgttcgg ataaaccatt
    22501 ccataaccga agccgttacc gcgcgcctga tgttttttcg catatcgata gaggtacttc
    22561 cacagcaccg gcgtcaggat atatttcgcc tcgaccatcg ggtccaacag ctgcgccagc
    22621 gtcactcgct gtgcagggaa acattcgctg atatcacgca gagtaaaatc ggctttcaga
    22681 ttaagatcgc gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg cagaaaatgt
    22741 ttaccatcga tgattttcgg atcgtccggc ccgttatctt ctgcatcagc cacgtcatag
    22801 cccagttcgt ccagcgtctg catgatgatg cggaacgttt taccctggtc gtgacttttc
    22861 aggtttttga cgttttcgag cacaaacatc gccggacgac gcgcgtcgat aatgcgtacc
    22921 acatcaaaaa acagcgtgcc ctgggtatcg caggcaaaac cgtgcgcccg cccgagcgag
    22981 ttctttttcg atacgccagc cagcgaaaat ggctgacaag ggaaaccggc cagtaaaaca
    23041 tcgtgttcag gaatgtgttg acgaatatgt tccgccgccg cctcatcact cacgccttct
    23101 ttatggctga gggtgatatc gcggatatct tcattaaaat gatgcgtcgc cggatcgcaa
    23161 taatggttgg ctttataagt gcgtaccgca tgtttgttcc attcgctggt aaacacgcat
    23221 tgtccgccaa tcgattcaaa accgcgacgg atgccgccaa ttccggcaaa tagatcgata
    23281 aagcgaaacg cataatgcgg atgatgtgcc ggtggtttgg gtaacaacgt ttgcagatgg
    23341 gcgaactctt tctcacttaa acggtgccat gccgagtcat tcgccagcgc acgttttaaa
    23401 attgccgcgc tccagtgatt ctcacccaca ccattaagct gcgccaccag cgttttaaca
    23461 tcataaattt gcagcagttt ctccagcatt gcctgtgcgg cattcccggt gctgtatgaa
    23521 tcggttactg atatattttc ctgcatagat ttaaccggcc atctgagatg gccgacaggt
    23581 taacataatt acagccattt agggccacgc gcttatcccg cgggtgaaaa cgcatctatc
    23641 accttgtgat cgacaccttc attctccccc gccagttcgg cactgagctt cgccataaac
    23701 tcgactaaaa aatgcgcgtt gtgctgcgcc agctgcttgc ctcttgcggt ttgcatggtt
    23761 tgcggtagct tcagcaactt agtctggaaa tgatccagcg cgtagcgttt atcatcaagc
    23821 gggcgatgct gtgcgaacgg atcttcacca tcaaacagcg ccacgcccaa tgcccctgaa
    23881 acggcaaaca cgcgcgccag gccaatcgcc cccagggctt ccagccgatc cgcatcctgg
    23941 acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg agcggcaatg
    24001 gcatgacaaa cggcctcgat tttctccgcc ggaaattgct cgaactcttc acggagcagg
    24061 cgacgcgtct cttctgccgc caggattgaa gaacgctgcc tttgcggatg atttttcgcc
    24121 aggctcacaa tatcgtgaaa ataacaggcg gttaaaatca ccagcatatc gacatcgtca
    24181 tctgccgcca gcttctgtgc cgtagcccag acgcggcgaa aatgacacac gtcatgggcc
    24241 gcgtcctggt gctgatgatg gtttttcaac cagttttcga actgtgcctg ccagtgttgt
    24301 aagtccatac gcgctccgtt gccaaagagt cgccacctta gcagttttta ttcatagcgt
    24361 ctgctacgtg cgggacgagg tcgggtgtac caggcaatgc tgcctaacaa tgccccgacc
    24421 agagccataa ataaaaatcc gaccagtgcc cctaaccact ttcccgcggt agagcctaaa
    24481 tcgctttcaa atcccgacac gggggcatcg gggatccggc tcatcaccca gatgtaacgc
    24541 gccattgccc agacgaaata gccacaccat gtatagaaag cccacaaagc cggtttgccg
    24601 ccagggctgc gagagagtct ctcttccatc tttgtcgaac cgtattccat tttgagataa
    24661 cccggaaaat tcagacttag tactattatg tgataaaagt cacatttttc cacattggat
    24721 gagccagtca agactcaatc attttttgaa caaatagata aattttgcgc aacggcagtg
    24781 aatacaggaa aaatcgtttt gagcgatttt atttatcttt gatatcggct t
//
LOCUS       DAZFZC010000079        24542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFZC010000079 DAZFZC010000000
VERSION     DAZFZC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.079"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..1107
                     /gene="ybeQ"
                     /locus_tag="ACHKXP_003518"
     CDS             130..1107
                     /gene="ybeQ"
                     /locus_tag="ACHKXP_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO7648459.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1177..1659)
                     /gene="ybeL"
                     /locus_tag="ACHKXP_003519"
     CDS             complement(1177..1659)
                     /gene="ybeL"
                     /locus_tag="ACHKXP_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO7648460.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1894..4476
                     /gene="leuS"
                     /locus_tag="ACHKXP_003520"
     CDS             1894..4476
                     /gene="leuS"
                     /locus_tag="ACHKXP_003520"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO7648461.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGINSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTENQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4491..5072
                     /gene="lptE"
                     /locus_tag="ACHKXP_003521"
     CDS             4491..5072
                     /gene="lptE"
                     /locus_tag="ACHKXP_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO7648462.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5072..6103
                     /gene="holA"
                     /locus_tag="ACHKXP_003522"
     CDS             5072..6103
                     /gene="holA"
                     /locus_tag="ACHKXP_003522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO7648463.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNGAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6105..6746
                     /gene="nadD"
                     /locus_tag="ACHKXP_003523"
     CDS             6105..6746
                     /gene="nadD"
                     /locus_tag="ACHKXP_003523"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO7648464.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6770..7381
                     /gene="cobC"
                     /locus_tag="ACHKXP_003524"
     CDS             6770..7381
                     /gene="cobC"
                     /locus_tag="ACHKXP_003524"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO7648465.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7641..7958
                     /gene="rsfS"
                     /locus_tag="ACHKXP_003525"
     CDS             7641..7958
                     /gene="rsfS"
                     /locus_tag="ACHKXP_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO7648466.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7962..8429
                     /gene="rlmH"
                     /locus_tag="ACHKXP_003526"
     CDS             7962..8429
                     /gene="rlmH"
                     /locus_tag="ACHKXP_003526"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO7648467.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8460..10361
                     /gene="mrdA"
                     /locus_tag="ACHKXP_003527"
     CDS             8460..10361
                     /gene="mrdA"
                     /locus_tag="ACHKXP_003527"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO7648468.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10364..11476
                     /gene="mrdB"
                     /locus_tag="ACHKXP_003528"
                     /gene_synonym="rodA"
     CDS             10364..11476
                     /gene="mrdB"
                     /locus_tag="ACHKXP_003528"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO7648469.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11487..12575
                     /gene="rlpA"
                     /locus_tag="ACHKXP_003529"
     CDS             11487..12575
                     /gene="rlpA"
                     /locus_tag="ACHKXP_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO7648470.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAVSQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12714..13925
                     /gene="dacA"
                     /locus_tag="ACHKXP_003530"
     CDS             12714..13925
                     /gene="dacA"
                     /locus_tag="ACHKXP_003530"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO7648471.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14035..14298
                     /gene="ybeD"
                     /locus_tag="ACHKXP_003531"
     CDS             14035..14298
                     /gene="ybeD"
                     /locus_tag="ACHKXP_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO7648472.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14399..15040
                     /gene="lipB"
                     /locus_tag="ACHKXP_003532"
     CDS             14399..15040
                     /gene="lipB"
                     /locus_tag="ACHKXP_003532"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO7648473.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15299..16252
                     /gene="ybeF"
                     /locus_tag="ACHKXP_003533"
     CDS             15299..16252
                     /gene="ybeF"
                     /locus_tag="ACHKXP_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO7648474.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPAPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16461..17426
                     /gene="lipA"
                     /locus_tag="ACHKXP_003534"
     CDS             16461..17426
                     /gene="lipA"
                     /locus_tag="ACHKXP_003534"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO7648475.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17527..17730)
                     /gene="tatE"
                     /locus_tag="ACHKXP_003535"
     CDS             complement(17527..17730)
                     /gene="tatE"
                     /locus_tag="ACHKXP_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO7648476.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17859..18647)
                     /locus_tag="ACHKXP_003536"
     CDS             complement(17859..18647)
                     /locus_tag="ACHKXP_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO7648477.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18740..19123
                     /gene="crcB"
                     /locus_tag="ACHKXP_003537"
     CDS             18740..19123
                     /gene="crcB"
                     /locus_tag="ACHKXP_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO7648478.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19177..19386)
                     /gene="cspE"
                     /locus_tag="ACHKXP_003538"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19177..19386)
                     /gene="cspE"
                     /locus_tag="ACHKXP_003538"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7648479.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19561..20121)
                     /gene="pagP"
                     /locus_tag="ACHKXP_003539"
     CDS             complement(19561..20121)
                     /gene="pagP"
                     /locus_tag="ACHKXP_003539"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO7648480.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20709..22094
                     /gene="dcuC"
                     /locus_tag="ACHKXP_003540"
     CDS             20709..22094
                     /gene="dcuC"
                     /locus_tag="ACHKXP_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7648481.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(22135..22815)
                     /gene="dpiA"
                     /locus_tag="ACHKXP_003541"
                     /gene_synonym="citB"
     CDS             complement(22135..22815)
                     /gene="dpiA"
                     /locus_tag="ACHKXP_003541"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO7648482.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(22784..24442)
                     /gene="dpiB"
                     /locus_tag="ACHKXP_003542"
     CDS             complement(22784..24442)
                     /gene="dpiB"
                     /locus_tag="ACHKXP_003542"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO7648483.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIVEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6161 a         6182 c         6071 g         6128 t
ORIGIN      
        1 tagccgcatt gcgaggctcg tttatttata cttttctgac gcgacataat acgcataaaa
       61 taaggcttac gcaaaaatgg aacacataaa aatataatat gttttatctg caaccataag
      121 gaaatgataa tgattttcac gtcaagttgc tgcgataatt tatcaataga tgagattatc
      181 gaacgtgctg aaaaaggcga ttgcgaggct caatatattg ttgggtttta ttataatcgc
      241 gatagcgcaa ttgattctcc ggacgacgaa aaagcctttt actggctgaa gctggccgct
      301 gagcaaggtc attgtgaagc acagtattcc ttagggcaaa agtataccga ggataaaagc
      361 cgtcataaag ataatgagca agccatcttc tggctgaaaa aagctgccct acaaggccat
      421 actttcgctt ccaacgccct tggctggaca ctggatcgtg gagaagcccc caattataaa
      481 gaagcggttg tctggtatca gatagccgcg gagagcggaa tgtcttatgc gcaaaataat
      541 cttgggtgga tgtacagaaa tggcaacgga gtcgcaaaag actatgcgct ggcatttttc
      601 tggtacaaac aagctgcatt acaaggccat agtgacgcgc aaaacaatct ggccgatctt
      661 tatgaagacg gaaaaggcgt tgctcaaaac aagacactcg ccgcattctg gtatttgaaa
      721 agcgcacagc agggtaatcg gcacgcccag tttcaaattg catgggatta taacgctggc
      781 gaaggggtgg accaggacta taagcaagcg atgtactggt atctgaaggc tgccgctcag
      841 gggagcgtcg gcgcttacgt caacatcggt tatatgtata aacacggaca aggcgttgag
      901 aaagattatc aggctgcctt tgaatggttt acgaaagccg ctgaatgcaa tgacgccact
      961 gcctggtata acctggccat tatgtatcac tacggagaag gaagacctgt cgatctccga
     1021 caggctctcg acctgtatcg taaagttcag tcatccggaa ccagagatgt cagtcaggaa
     1081 attcgtgaga ctgaagattt actgtagaga taaaaacaag ggccggggaa tttcattcgg
     1141 cccatattct ctttattgct gtgcgaaatc agactattac ggctcaaacg ggcgtctctg
     1201 gaactggtca tgaccacatt tcgggcatag cgtcagcact tccggtgtgt agatcgggag
     1261 atggaagtga catttctcgc agaccagatt tcccagcccg accacttctc cgctgtgata
     1321 aaccccatga tgattgaggt cctggaaaac ttcgcgccat tcaagctgcg ttttatcggt
     1381 gatgtctgcc agctcctgcc acaagctttc tttaatcacc cgcataaaga cgctgtcaga
     1441 ttcttctttc aggctctctt catagctcat ggcgaactct tccaggtcac gtctgacagc
     1501 tcgcgtcagc tcatcgacct cggttcgcgt taactcccct gtttttatta cgcgctcgcg
     1561 cgcctgttcc accagtgcgt cgatatcacg ttcgccattg cgcaggcgtt cgctcagtga
     1621 cgcgaccagt tcacggtaat attgagcaac cttgttcatc gtttcatctc ccgggtcgtt
     1681 aactgactat aataatagac gttctttgtt ccttgctttg ctaatacgcc cacaaactct
     1741 gtaaattaat gtacgaataa tccgggcatc atttcaacgt cgagtgcgcg aaaggctgtt
     1801 ttgacgcgga cgtttgggct atgctatgcg gatctgaaaa accacctcaa cgctacattt
     1861 gtagccgtat tgaaaacagg accactggct gccatgcaag agcaataccg cccggaagag
     1921 atagaatcca aagtacagct tcattgggat gagaagcgca catttgaagt aaccgaagac
     1981 gagagcaaag agaagtatta ctgcctgtct atgcttccct atccttctgg tcgactacac
     2041 atgggccacg tacgtaacta caccatcggt gacgtgatcg cccgctacca gcgtatgctg
     2101 ggcaaaaacg tcctgcagcc gatcggctgg gacgcgtttg gtctgcctgc ggaaggcgcg
     2161 gcggtgaaaa acaacaccgc tccggcaccg tggacgtacg acaacatcgc gtatatgaaa
     2221 aaccagctca aaatgctggg ctttggttat gactggagcc gcgagctggc aacctgtacg
     2281 ccggaatact accgttggga acagaaattc ttcaccgagc tgtataaaaa aggcctggta
     2341 tataagaaga cttctgcggt caactggtgc ccgaacgacc agaccgtact ggcgaacgaa
     2401 caagttatcg acggctgctg ctggcgctgc gataccaaag ttgaacgtaa agagatcccg
     2461 cagtggttta tcaaaatcac tgcttacgct gacgagctgc tcaacgatct ggataaactg
     2521 gatcactggc cagacaccgt taaaaccatg cagcgtaact ggatcggtcg ttccgaaggc
     2581 gtggagatca ccttcaacgt taacgactat gacaacacgc tgaccgttta cactacccgc
     2641 ccggacacct ttatgggttg tacctacctg gcggtagctg cgggtcatcc gctggcgcag
     2701 aaagcggcgg aaaataatcc tgaactggcg gcctttattg acgaatgccg taacaccaaa
     2761 gttgccgaag ctgaaatggc gacgatggag aaaaaaggcg tcgatactgg ctttaaagct
     2821 gttcacccat taacgggcga agaaattccc gtttgggcag caaacttcgt attgatggag
     2881 tacggcacgg gcgcagttat ggcggttccg ggtcacgacc agcgcgacta cgagtttgcc
     2941 tctaaatacg gcctgaacat caaaccggtt atcctggcag ctgacggctc tgagccagat
     3001 ctttctcagc aagccctgac tgaaaaaggc gtgctgttca actctggcga gttcaacggt
     3061 cttgaccatg aagcggcctt caacgccatc gccgataaac tgactgcgat gggcgttggc
     3121 gagcgtaaag tgaactaccg cctgcgcgac tggggtgttt ctcgtcagcg ttactggggc
     3181 gcgccgattc cgatggtgac gctggaagac ggtaccgtaa tgccgacccc ggacgaccag
     3241 ctgccggtga tcctgccgga agatgtggtg atggacggca ttaacagccc gattaaagca
     3301 gatccggagt gggcaaaaac taccgttaac ggtatgccag cgctgcgtga aaccgacact
     3361 ttcgacacct ttatggagtc ctcctggtac tatgcgcgct acacttgccc agagtacaaa
     3421 gaaggtatgc tggattccga agcggctaac tactggctgc cggtggatat ctacattggt
     3481 ggtattgaac acgccattat gcacctgctc tacttccgct tcttccacaa actgatgcgt
     3541 gatgcaggca tggtgaactc tgacgaacca gcgaaacagt tgctgtgtca gggtatggtg
     3601 ctggcagatg ccttctacta tgttggcgaa aacggcgaac gtaactgggt ttccccggtt
     3661 gatgctatcg ttgaacgtga cgagaaaggc cgtatcgtga aagcgaaaga tgcggcaggc
     3721 catgaactgg tttataccgg catgagcaaa atgtccaagt cgaagaacaa cggtatcgac
     3781 ccgcaggtga tggttgaacg ttacggcgcg gacaccgttc gtctgtttat gatgtttgct
     3841 tctccggctg atatgactct cgaatggcag gaatccggtg tggaaggggc taaccgcttc
     3901 ctgaaacgtg tctggaaact ggtttacgag cacacagcaa aaggtgatgt tgcggcactg
     3961 aacgttgatg cgctgactga aaatcagaaa gcgctgcgtc gcgatgtgca taaaacgatc
     4021 gctaaagtga ccgatgatat cggtcgtcgt cagaccttca acaccgcaat tgcggcgatt
     4081 atggagctga tgaacaaact ggcgaaagca ccaaccgatg gcgagcagga tcgcgctctg
     4141 atgcaggaag cgctgctggc cgttgtccgt atgcttaacc cgttcacccc gcacatctgc
     4201 ttcacgctgt ggcaggaact gaaaggcgaa ggcgatatcg acaacgcgcc gtggccggtt
     4261 gctgacgaaa aagcgatggt ggaagactcc acgctggtcg tggtgcaggt taacggtaaa
     4321 gtccgtgcca aaatcaccgt tccggtggac gcaacggaag aacaggttcg cgaacgtgct
     4381 ggccaggaac atctggtagc aaaatatctt gatggcgtta ctgtacgtaa agtgatttac
     4441 gtaccaggta aactcctcaa tctggtcgtt ggctaagcgc gggaggaagc gtgcgatatc
     4501 tggcaacatt gttgttatct ctggcggtgt taatcaccgc cgggtgtggc tggcatctgc
     4561 gtgataccac gcaggttcct tccactatga aggtcatgat cctggactca ggcgatccga
     4621 acgggccatt aagccgtgcg gtgcgtaacc agttacgtct gaatggtgtc gagttgcttg
     4681 ataaagaaac cacgcgtaag gacgttccat ccctgcgttt gggtaaagtg agcatcgcga
     4741 aagataccgc atcggtattc cgtaacggtc aaacagcaga gtatcagatg atcatgacgg
     4801 tgaatgcgac cgtgttgatc cccggccgtg atatctaccc gattagcgcc aaagtcttcc
     4861 gttcgttctt tgataacccg caaatggcgt tagcgaaaga taacgaacaa gacatgatcg
     4921 taaaagagat gtacgaccgt gctgccgaac agctgattcg taagctgcca agcatccgtg
     4981 ctgcggatat tcgttccgac gaagaacaga cgtcgaccac aacggatact ccggcaacgc
     5041 ctgcccgcgt ctccaccacg ctgggtaact gatgattcgg ttgtacccgg aacaactccg
     5101 cgcgcagctc aatgaagggc tgcgcgcggc gtatctttta cttggtaacg atcctctgtt
     5161 attgcaggaa agccaggacg ctgttcgtca ggtagctgcg gcacaaggat tcgaagaaca
     5221 ccacactttt tccattgatc ccaacactga ctggaatgcg atcttttcgt tatgccaggc
     5281 tatgagtctg tttgccagtc gacaaacgct attgctgttg ttaccagaaa acggaccgaa
     5341 tggggcgatc aatgaacaac ttctcacact caccggactt ctgcatgacg acctgctgtt
     5401 gatcgtccgc ggtaataaat taagcaaagc gcaagaaaat gccgcctggt ttactgctct
     5461 tgcgaatcgc agcgtgcagg tgacctgtca gacaccggag caggctcagc ttccccgctg
     5521 ggttgctgcg cgcgcaaaac agctcaactt agaactggat gacgcggcga atcaggtgct
     5581 ctgctactgt tatgaaggta acctgctggc gctggctcag gcactggagc gtttatcgct
     5641 gctctggcca gacggcaaac tgacattacc gcgcgttgaa caggcggtga atgatgccgc
     5701 gcatttcacc ccttttcatt gggttgatgc tttgttgatg ggaaaaagta agcgcgcgtt
     5761 gcatattctt cagcaactgc gtctggaagg cagcgagccg gttattttgt tgcgcacatt
     5821 acaacgtgaa ctgttgttac tggttaacct gaaacgccag tctgcccata cgccactgcg
     5881 tgcattgttt gataagcatc gggtatggca gaaccgccgg ggcatgatgg gcgaggcgtt
     5941 aaatcgctta agtcagccgc agttacgtca ggccgtgcaa ctccttacac gaacggaact
     6001 caccctcaaa caagattacg gtcagtcagt gtgggcagag ctggaagggt tatctcttct
     6061 gttgtgccat aaacccctgg cggacgtatt tatcgacggt tgatatgaaa tctttacagg
     6121 ctctgtttgg cggcaccttt gatccggtgc actatggtca tctaaaaccc gtggaaacgc
     6181 tggcgaattt gattggtctg acgcgggtca caatcatccc taataatgtt cctccgcatc
     6241 gtccccagcc ggaagcgaac agcgtgcagc gtaaacacat gcttgaactg gcgattgccg
     6301 acaagccatt atttactctt gatgaacgcg agctaaagcg caatgccccc tcttacactg
     6361 cgcaaacact gaaagagtgg cggcaggaac aaggaccgga cgtgccgctg gcgtttatta
     6421 ttggtcagga ttcattgctg acctttccga cctggtacga atacgaaacg atactcgaca
     6481 atgcacattt gatcgtctgt cggcgtccag gttacccact tgaaatggcg caaccgcaat
     6541 accagcaatg gctggaagat catttgacac ataacccgga agatcttcac cttcagcctg
     6601 ccggtaaaat ttatctggct gaaacgccgt ggtttaacat ctcggcgacc atcatccgcg
     6661 aacgtttgca aaacggtgaa tcgtgtgagg atttattgcc ggaaccggta ttgacttaca
     6721 ttaaccaaca aggcttgtat cgctgatacc tgtcgttacg gagggcatga tgcgactgtg
     6781 gttaattcgt catggtgaaa cgcaagcgaa tatcgatggt ctttacagcg gtcatgcgcc
     6841 cacccccctg accgcgcgcg gtattgagca agcgcaaaat ctgcatacgc tgctacatgg
     6901 tgtttccttt gatctggttt tatgcagtga actggaacgg gcacagcata ccgcgcgact
     6961 ggttctcagt gaccgccagc tccccgtgca aatcatacct gaactcaacg aaatgttttt
     7021 tggcgactgg gagatgcgac atcatcgcga cctcatgcaa gaagatgccg aaaactatag
     7081 cgcgtggtgc aatgactggc agcatgcaat ccccacgaac ggtgaaggat ttcaggcatt
     7141 ttcgcaacgt gtggaacgct ttatcgcaag gcttagtgaa tttcagcact atcagaatat
     7201 tttagtcgtc agccatcagg gtgtactgag tctgttaatc gcccgtttaa ttggcatgcc
     7261 tgccgaggct atgtggcatt ttcgcgttga ccaggggtgt tggagcgcca ttgatatcaa
     7321 ccaaaaattc gcgacgctac gcgtcctcaa tagccgtgcc atcggggtcg agaatgcatg
     7381 acttttctgt ttttttacgg gtaagccgca acgaccattg acagacccgg gcaggctgat
     7441 attctccgca gccagacttt ttccgccaga cacgactttg tagaaattgt tttacaaaaa
     7501 tggcgatgca atctgcggcg cggggtggga tgatagccca ctttcgaaag ccgattcggc
     7561 gacaattgtc ccgaaatcgc ctctggttca ggtatactga cagaccattt ttatctattt
     7621 gattcaccca gggggaaaac ttgcagggta aagcactcca ggattttgtt atcgacaaaa
     7681 ttgatgacct caaaggtcag gacatcatcg ccttagacgt tcagggcaaa tccagcatca
     7741 ccgactgcat gatcatctgt acgggtacgt ccagccgtca tgttatgtcc attgctgacc
     7801 acgttgtgca ggagtctcgc gcagcgggcc tgttaccgct cggcgtagaa ggtgaaaaca
     7861 gcgccgactg gattgtcgtg gatttgggcg atgtgattgt ccatgtcatg caggaagaga
     7921 gccgtcgcct gtatgaactg gaaaaactct ggagttaatg cgtgaagctg caacttgtcg
     7981 ccgtgggaac gaaaatgccg gactgggtac aaaccggttt taccgagtac ctgcgtcgtt
     8041 ttccgaaaga tatgcccttc gagctgattg aaattccggc cggaaaacgc ggcaagaatg
     8101 cggacatcaa gcgcatactc gacaaagagg gtgagcagat gttggcggcc gcaggcaaaa
     8161 accgcattgt caccctcgat attccaggca agccctggga tacgccgcag ttagccgctg
     8221 agctggaacg ctggaagctg gatggtcgcg acgtcagtct actgattggc gggcctgaag
     8281 ggttgtcgcc tgcctgtaaa gcggcggctg agcagagctg gtcgctgtcg gcgcttaccc
     8341 tcccccatcc gctggttcgc gtgctggtcg cagagagtct gtaccgggcg tggagcatca
     8401 ccaccaacca tccttatcac cgtgagtgat aagggagctt tgagtagaaa acgcagcgga
     8461 tgaaactaca gaactctttt cgcgactata cggcagagtc cgcgctgttt gtgcgccggg
     8521 cgctggtcgc ctttttgggg attttgctgc tgaccggcgt gcttatcgcc aacctgtata
     8581 atctgcaaat tgttcgcttt accgactacc agacccgctc taatgaaaac cgcattaagc
     8641 tggtgcctat cgcgcccagc cgcggcatta tctacgatcg taacggtatc cctctggccc
     8701 tcaaccgcac tatctaccag atagaaatga tgccggagaa agtcgataac gtgcagcaaa
     8761 cgctggacgc tttgcgcagc gtggtagatc tgaccgatga cgatattgct gcattccgaa
     8821 aagagcgcgc acgttcacac cgtttcacct ctattccggt gaaaactaac ctgaccgaag
     8881 tacaagtagc tcgctttgcc gtcaatcagt accgttttcc gggtgtcgaa gttaaaggct
     8941 ataaacgtcg ttactatcct tacggttcgg cgttgaccca cgtcatcggc tatgtgtcga
     9001 aaatcaacga taaagacgtc gaacgcctga ataatgacgg caaactggcc aactatgcgg
     9061 caacgcatga tatcggtaag ctgggcattg agcgttacta tgaagatgtg ctgcacggtc
     9121 agaccggtta tgaagaggtt gaagttaaca accgtgggcg tgttattcgc cagttaaaag
     9181 aagtaccacc gcaagccgga cacgatattt acctgacgct ggatctcaaa ctccagcaat
     9241 atattgaaac gctgctggcg ggtagccgcg cagctgtggt agtcaccgat ccgcgtacag
     9301 gtggggtgct ggcgctggtt tccacgccta gttatgaccc aaacttgttt gttgacggta
     9361 tctccagcaa agattattcc gccttgttga acgatccgaa tacaccgctg gtgaaccgcg
     9421 ccacacaggg ggtttatcct cccgcgtcta cagttaaacc ctatgtggcg gtttcggcat
     9481 tgagcgccgg ggtgatcacg cgcaatacga cgctgtttga cccaggctgg tggcaactgc
     9541 caggttcgga aaaacgttat cgtgactgga aaaaatgggg ccacgggcgt ctgaatgtca
     9601 caagatcgct ggaagaatct gcggatacct tcttctatca ggtggcctac gatatgggga
     9661 tcgatcgcct ctccgaatgg atgggtaaat tcggttatgg tcattacacc ggtatcgacc
     9721 tggcggaaga acgttccggc aacatgccta cccgcgaatg gaaacagaaa cgctttaaaa
     9781 aaccgtggta tcagggtgac accattccgg ttggtatcgg tcagggttac tggacagcga
     9841 ccccaatcca gatgagtaag gcactgatga tcctgattaa tgacggtatc gtgaaggttc
     9901 ctcatttgct gatgagcacc gccgaagacg gcaaacaggt gccatgggta cagccgcatg
     9961 aaccgcccgt cggcgatatt cattccggtt actgggagct ggcgaaagac ggtatgtacg
    10021 gtgttgctaa ccgccctaac ggtacggcgc ataaatactt tgctagcgca ccgtacaaaa
    10081 ttgcggcgaa atccggtacc gctcaggtct tcggtctgaa agcgaacgaa acctataatg
    10141 cgcacaaaat tgccgagcgt ttacgtgacc acaaactgat gaccgccttt gcgccataca
    10201 acaatccgca agtggctgtc gccatgattc tggagaacgg tggtgcgggt ccggcggttg
    10261 gtacactgat gcgccagatc ctcgaccaca ttatgctggg tgataacaac accgatctgc
    10321 ctgcggaaaa tccagcggtt gccgcagcgg aggaccatta atcatgacgg ataatccgaa
    10381 taaaaaaaca ttctgggata aagtccatct cgatcccaca atgctgctga tcttactggc
    10441 attgctggtt tacagcgccc tggttatctg gagcgccagc ggtcaggata ttggcatgat
    10501 ggagcgtaaa atcggccaaa tcgcgatggg tctggtcatc atggtggtga tggcgcaaat
    10561 tcctccacgc gtttatgaag gctgggcccc ctatctctat atcatctgta ttattttgct
    10621 ggtggcggta gatgctttcg gtgccatctc taaaggtgct caacgctggc tggacctcgg
    10681 tattgttcgt tttcagccgt cggaaattgc caaaatagcc gtaccactga tggttgcgcg
    10741 ctttatcaac cgcgacgttt gcccgccatc gttgaagaac actggcatcg cgctggtgct
    10801 gatatttatg cccacgctgc tggtggctgc acagcctgac ctgggaacat caatcctcgt
    10861 tgcgctttcc ggtctgtttg tactgttcct ctctggcctt agctggcgtc tgattggcgt
    10921 cgcagtagtg ctggtagcgg cgttcattcc gattctgtgg ttcttcctga tgcatgatta
    10981 ccagcgccag cgcgtaatga tgctcctgga cccggaatca gacccactcg gcgcgggcta
    11041 tcacattatt cagtctaaaa ttgctattgg ctccggcgga ttacgcggca aaggctggct
    11101 gcacggcact cagtcacagc ttgaatttct ccccgaacgc catactgact ttatcttcgc
    11161 ggtactggcg gaagagctgg gattagtggg cattctgatt ctgctcgctc tctacattct
    11221 gctgatcatg cgcgggctgt ggatagccgc cagagcgcaa accacctttg gtcgcgtcat
    11281 ggctggcggc ttaatgctga tattattcgt ttatgtcttc gtaaatattg gtatggtaag
    11341 cggtattctg ccggttgtag gggttccgct cccactggtc agttatggag gatcggcgct
    11401 aattgtgctg atggctgggt tcgggattgt aatgtcaatc cacacccaca ggaaaatgtt
    11461 gtcgaaaagc gtgtaagagg tgcgcaatgc gtaagcagtg gctcgggatc tgcatcgcgg
    11521 caggaatgct cgcggcatgt acaagcgatg atggtcagca acagacggta agtgtaccgc
    11581 agcctgcggt atgtaacggc cctatagttg aaattagcgg ggcggacccg cgtttcgaac
    11641 cactgaacgc gacggcaaat caggattacc agcgcgacgg taaaagctac aaaatcgtgc
    11701 aggatccgtc tcgatttagc caggcgggac tggcggcaat ctatgatgcc gaacccggca
    11761 gtaacctgac ggcctctggc gaagcattcg atccgacaca gctgacggca gcccatccaa
    11821 cgcttccgat ccccagctac gccagaatca ctaacctggc taacgggcga atgatcgtgg
    11881 tgcgcattaa tgatcgcggc ccttacggca acgaccgcgt tatttcactt tctcgcgcgg
    11941 cagctgaccg tcttaacacg tcaaacaaca ccaaagttcg tatcgatccg attattgttg
    12001 cccaggatgg ttcgctttct ggtcctggta tggcttgtac cacagtcgcc aaacagactt
    12061 acgccctgcc tgcacctccc gatttaagcg gtggcgcggg aacaagttca gtgtctggcc
    12121 cgcagggtga cattcttccg gtcagtaatt cgacgctaaa aagcgaagat ccgaccggcg
    12181 cgccggtaac cagtagcggt ttcctcggcg caccaacgac cttagcgcct ggtgtactgg
    12241 aaggcagcga accgacgcct gcgccacagc ccgttgttac agctccgtcg acaacgcctg
    12301 caacctcgcc tgcaatggtg acaccgcaag ccgtctcgca aagcgccagc ggcaacttta
    12361 tggtgcaagt cggggccgta agcgatcagg ctcgtgcgca acagtaccaa cagcaactgg
    12421 gacagaagtt cggcgtcccc ggtcgcgtaa ctcaaaatgg cgcggtctgg cggatccagc
    12481 ttggcccatt cgccagcaaa gccgaagcca gtaccttgca gcaacgtttg caaaccgaag
    12541 cccaattaca gtcatttatt actaccgcgc agtagcgtaa agcaggcatc tgatgtgtca
    12601 atttctgtaa atgacgttaa caaagtcacg cggaaagtca gatgcctgcc ggtagtgcat
    12661 ttgctatagt agggcacttt tttaattcca tcacggatgt cgtagttcag accatgaata
    12721 ccattttttc cgctcgtatc atgaagcgcc tggcgctcac cacggctctt tgcacagcct
    12781 ttatctctgc tgcacatgcc gatgacctga atatcaaaac tatgatcccg ggtgtaccgc
    12841 agatcgatgc ggagtcctac atcctgattg actataactc cggcaaagtg ctcgccgaac
    12901 agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc agttacgtta
    12961 tcggccaggc aatgaaagcc ggtaaattta aagaaactga tttagtcact atcggcaacg
    13021 acgcatgggc caccggtaac ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg
    13081 gcatgcaggt tccggtttct cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg
    13141 cttgtgtcgc catggccgat tttgccgctg gtagccagga cgcttttgtt ggcttgatga
    13201 acagctacgt taacgcactg ggcctgaaaa atacccactt ccagacggta catggtctgg
    13261 atgctgatgg tcagtacagc tccgcgcgag atatggcgct gatcggccag gcattgatcc
    13321 gtgacgtacc gaatgaatac tcgatctata aagaaaaaga atttacgttt aacggtattc
    13381 gccagctgaa ccgtaacggc ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa
    13441 ccggacacac tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa ggccagatgc
    13501 gcttgatttc tgcggtaatg ggcggacgta cttttaaagg ccgtgaagcc gaaagtaaaa
    13561 aactgctaac ctggggcttc cgtttctttg aaaccgttaa cccactgaaa gtaggtaaag
    13621 agttcgcctc tgaaccggtt tggtttggtg attctgatcg cgcttcgtta ggggttgata
    13681 aagacgtgta cctgaccatt ccgcgtggtc gcatgaaaga tctgaaagcc agctatgtgc
    13741 tgaacagcag tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga actatcaact
    13801 tccagcttga tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa gaaatcccgg
    13861 aaggtaactt cttcggcaaa atcattgatt acattaaatt aatgttccat cactggtttg
    13921 gttaaaaatt aaacacttga aagtgtaatt tccgtcccca tatactaagc atcagtaaaa
    13981 aactcccgcc ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa
    14041 accaaactta acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag
    14101 gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac
    14161 tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc
    14221 aacgccactc atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt
    14281 gtccgcatgg ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct
    14341 ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt
    14401 gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca
    14461 ggctatgcat gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt
    14521 cgagcactat ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc
    14581 gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg
    14641 gcaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact
    14701 ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca
    14761 tcctcgggct gacgcgccag gtgtctatgt tggggaaaag aaaatttgct cactgggttt
    14821 acgtattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc
    14881 accattttta cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca
    14941 atggaaaccc gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc
    15001 gctactaaac aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca
    15061 atttacactg ggtcattact cataatatct gctaatgctt tacatctcct tcgcgtttgt
    15121 atattttctt tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat
    15181 tgattaacct attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca
    15241 taagattatt gtaatttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt
    15301 ggatagtaat aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca
    15361 aatatttaca accttacgaa atattgatct taaccttctg actatttttg aagctgtata
    15421 tgtacataaa gggatcgtta atgcagcgaa agtgcttaat ctgaccccct cggcaatcag
    15481 tcagtctatt cagaaactgc gcgttatatt ccctgcccca ttgtttattc gcaaaggcca
    15541 gggtgtcact cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga
    15601 gtccattctt ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat
    15661 tgcgaccact ccctcggtcg gagcactggt ccttcctgtc atctatcggg cgattaaaac
    15721 tcactatccg cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag
    15781 tcagtttcaa accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca
    15841 tcatgttctg ttcaccgaca atatggtgtt aatttgccgc gagggaaatc cactactctc
    15901 tttagaagat gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaaga
    15961 gcaaaatttc agcggtctgc ggcagagagt tcaagaaatg tttccggacc ggcaaatcaa
    16021 tttcaccagc tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc
    16081 gattattccc agccgttttt ataacctgtt tagccgctgc tggccgctgg aaaaactgcc
    16141 ttttccgtcc ttaaatgagg agcaaataga tttctctatc cactacaaca aattcagcct
    16201 gcgtgatccc atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat
    16261 tgttacagcc agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca
    16321 ttttcgcgac cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa
    16381 ttcaaaaata gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc
    16441 gcaactggaa cacgcacgct atgagtaaac ccattgtgat ggaacgcgga gttaaatacc
    16501 gcgatgccga taagatggcc cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc
    16561 tgctgcgcaa gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg
    16621 gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc
    16681 ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
    16741 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca
    16801 atgaaccagt gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca
    16861 cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca gcactttgcg gattgcatta
    16921 ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgcg
    16981 gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc accagatgtg ttcaaccata
    17041 acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggtgcagat tacaactggt
    17101 cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
    17161 tgatggtggg actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc
    17221 gtcatggtgt gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc
    17281 cggttcaacg ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga
    17341 tgggctttac ccatgctgca tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc
    17401 aggcgaaagg gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa
    17461 aacccgcttt ttgaagcggg ttttttgtat cgaacaagat attgagggag cgtcctgctc
    17521 gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt
    17581 cgccgcagca tcgtcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc
    17641 tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac
    17701 cagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca
    17761 gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgcgcac acaatagcaa
    17821 tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca
    17881 aagcgacggt tgtttaagac gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc
    17941 acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga
    18001 tctataatcc ggctttgacc gatatttttg ttcccgcact cccccgccgc caccatataa
    18061 caggtggtat ccagcgcacg agcggcaagc aacgttgacc agtgatgctc tttgagcggc
    18121 ccgcgaaccc aggcggcagg aagtaccagg atttcagctc cctgtaatgc ctgtgccagc
    18181 gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccttccacc
    18241 tccagtaacg gagcgatttc attaccagca tcaacacggc gtgattcctg aatggcaaat
    18301 gcatcataga gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc
    18361 agcatattcc atgcgcgccc cggcgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc
    18421 atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct
    18481 gatttaaccg atagatctgc atcatgatca tcgcgcgcca gcaatgcttc cggcaggaca
    18541 aacagcgata cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc
    18601 ttttcccaca cagatgtaac agcaaactgt cctgcggcaa ccagcattgc cacctcctgt
    18661 cagagtcgta aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat
    18721 caaatagcag gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac
    18781 gggaagcgtg gcgagatggc tgttaagtat gcgatttaac ccactgcatc aggcgattcc
    18841 gttggggacg ctgacagcaa acctgattgg ggcattcatc ataggaatgg gattcgcatg
    18901 gttcagcagg atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg
    18961 tggcggtcta acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg
    19021 ccgctttggc tgggcattac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac
    19081 cgcactggca ttctggctgt tttcggcctc aaccgcacac taaagaccaa aaaaaacccg
    19141 ctgattaagc gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc
    19201 agaagggcct ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt
    19261 tttaaaacca ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc
    19321 cggagtaatg aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt
    19381 agacatcaaa attaccttta catgaaaaat agacacaaat gctgtgtcgg ttaccagtac
    19441 accaattgtg gtacgctttg tccagtgtaa ctttactaaa aagtgataaa agtcgcgtta
    19501 tttttgatgt tacttatttt agctattgat tttaaagaag ttactaaaac ttcatttgtc
    19561 tcaaaactga aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat
    19621 gtaggtcatc tgaaaagtca ctgggccata acccacggag gccaatggca gtagaaccgg
    19681 gagagggatg taattccagt tatcgcgtgc cgttacgcca gcggtgaatc ccagacctaa
    19741 atgaaaattt tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg
    19801 ttcccatttg ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt
    19861 ttcatcccaa cgcgacaggc caaaaccgcc accccacggt cgctcgttat agcgatcggt
    19921 tttttctttg tcgtaagcga aacgtgcatg ccaggtgatg gcaggaatat ataaatcata
    19981 atgttcaggc tgttgccagg tttgtgcaat attttctcta aacgttgtca tccactcatc
    20041 tgcgttagca aaaactttac caacgctgat taactgaata aaaacaaagg aaaagatagc
    20101 gacatattta ctcacgttca tttgtgacca taaaacattt atcaaaaatc tactactagc
    20161 atagcaaagc tacaattaac ataaccttaa tagacccaac ataaagaata atctgaatag
    20221 ctgctcattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    20281 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    20341 atgcaatcaa ggaaaaatta ttttattttt taacgagata caacaatcat cttaacgaag
    20401 tatataatat atatcgtcaa cctgtgctca tattagatta ttttctgagc gcacagccgc
    20461 agctaacacc attgcaatta acaaatttgc atcaatccac catcaatttg cacacattat
    20521 tatgtgataa ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagcaac
    20581 ttcaatgtct taatggcagt ttttcttgat tttaatcagc attcatcgac aatttattgg
    20641 gcatattttt tccttaagct ttaggaattt tttattattt actttggggc ctggagacag
    20701 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    20761 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    20821 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    20881 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    20941 acctcggcat gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg
    21001 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    21061 tgctgatgat tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa
    21121 ccggtctggg tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca
    21181 gtcgtggcgc agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    21241 caggggatgt ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca
    21301 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    21361 ggcaacgtta tctggataaa aaagaacaca tctctcatga aatgttagat gtcagtgaaa
    21421 tcaccaccac tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    21481 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    21541 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    21601 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    21661 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    21721 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    21781 ccatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    21841 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    21901 tgcaggcgtc aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg
    21961 ccgggatggc gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg
    22021 ttgggctagt gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg
    22081 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    22141 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    22201 atggcggctg gcgcaatatt caagataacg cctggcagtg gtgcgactga tggtcagtgc
    22261 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    22321 gtttagcgtc aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata
    22381 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    22441 tttacgctgg cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat
    22501 gagataatca aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc
    22561 agtggtaaac accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat
    22621 ccctctgccg tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat
    22681 catcattcgg gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg
    22741 acgaatatat tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt
    22801 taatggagct gtcattaggt ttcactttcg gaatatagat tgaaaacaag gtaccgcagg
    22861 gatcattatc ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt
    22921 acaacccaat gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata
    22981 ttttgtctcg tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat
    23041 catcgccttc atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc
    23101 tggcttcgaa ggcgttatca agtaaattgc ccacaatggc tgcaaactcg gtgctatcca
    23161 gtcctggcgg cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt
    23221 cccgggcgcg ctgcacttta ccaaaaagca gccccgccac ctggcgatcg gcaaacgcct
    23281 cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca
    23341 cgcgatcata ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct
    23401 cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat ttgcgtcagt tgcgcattga
    23461 gggtggaaat ttcgtcttta ctacgaaagc tgataatggc ccccagcaaa tcatcacctg
    23521 aacgaatagc ttcccggttg gcaataacgc tcagaccgtt aaagttcgcc accacatcct
    23581 gacgtttttc atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcaacaatgg
    23641 gtttacccaa ccattgccgt ccgggggaac tcagccccag catctttctt gcgttacgat
    23701 tgatggcggt aatgtaacca tgcggatcca ccgcaatcag cccttcataa accgaactaa
    23761 acagcgcctc ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcatct
    23821 gccgacggat atgcgcggcc aggaaccacg acagcaacat cagaatccct aacagcacga
    23881 caaacacacc tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca
    23941 ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg
    24001 cgcgcatcgc catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca
    24061 gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa
    24121 ggcggatcga gtgccggtcc ccaatcacca cataatcaaa atcggtatct ctttgtaatt
    24181 tgttagcgat ggtcgccagc cgtttgtagt cacgcgtttt caccgcagag atgacactgt
    24241 cattggaggc aataattttc gcctgattca ttgccatgtc gcgtacatga agcgttaaat
    24301 agtcctcaaa actggccgta aaatattgcg ccagggctgc aatgacaaat attgagaaca
    24361 ccagaatcag caaaaagata cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt
    24421 tattctcgtt aagctgcaac attgttttcc ttgcgggttg tcccgagcct ctttgacgga
    24481 tgtaattaaa atgttgaatc aaaatacata gatattaatt atgatcgtgg caaattttat
    24541 tc
//
LOCUS       DAZFZC010000080        23457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFZC010000080 DAZFZC010000000
VERSION     DAZFZC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.080"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(371..715)
                     /gene="yoaB"
                     /locus_tag="ACHKXP_003543"
     CDS             complement(371..715)
                     /gene="yoaB"
                     /locus_tag="ACHKXP_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7648484.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            847..2757
                     /gene="yoaA"
                     /locus_tag="ACHKXP_003544"
     CDS             847..2757
                     /gene="yoaA"
                     /locus_tag="ACHKXP_003544"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO7648485.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2815..3510
                     /gene="tsaB"
                     /locus_tag="ACHKXP_003545"
     CDS             2815..3510
                     /gene="tsaB"
                     /locus_tag="ACHKXP_003545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO7648486.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3550..4131
                     /gene="yeaY"
                     /locus_tag="ACHKXP_003546"
     CDS             3550..4131
                     /gene="yeaY"
                     /locus_tag="ACHKXP_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO7648487.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4336..6021
                     /gene="fadD"
                     /locus_tag="ACHKXP_003547"
     CDS             4336..6021
                     /gene="fadD"
                     /locus_tag="ACHKXP_003547"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO7648488.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6103..7218
                     /gene="rnd"
                     /locus_tag="ACHKXP_003548"
     CDS             6103..7218
                     /gene="rnd"
                     /locus_tag="ACHKXP_003548"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO7648489.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7272..8237)
                     /gene="yeaX"
                     /locus_tag="ACHKXP_003549"
     CDS             complement(7272..8237)
                     /gene="yeaX"
                     /locus_tag="ACHKXP_003549"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO7648490.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8293..9417)
                     /gene="yeaW"
                     /locus_tag="ACHKXP_003550"
     CDS             complement(8293..9417)
                     /gene="yeaW"
                     /locus_tag="ACHKXP_003550"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO7648491.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9449..10930)
                     /gene="yeaV"
                     /locus_tag="ACHKXP_003551"
                     /pseudo
     CDS             complement(9449..10930)
                     /gene="yeaV"
                     /locus_tag="ACHKXP_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
     gene            complement(11085..12170)
                     /gene="dmlA"
                     /locus_tag="ACHKXP_003552"
     CDS             complement(11085..12170)
                     /gene="dmlA"
                     /locus_tag="ACHKXP_003552"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO7648492.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12273..13196
                     /gene="dmlR"
                     /locus_tag="ACHKXP_003553"
     CDS             12273..13196
                     /gene="dmlR"
                     /locus_tag="ACHKXP_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO7648493.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYSQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGPHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            13323..13961
                     /gene="leuE"
                     /locus_tag="ACHKXP_003554"
     CDS             13323..13961
                     /gene="leuE"
                     /locus_tag="ACHKXP_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO7648494.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILAATL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            14134..14493
                     /gene="yeaR"
                     /locus_tag="ACHKXP_003555"
     CDS             14134..14493
                     /gene="yeaR"
                     /locus_tag="ACHKXP_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO7648495.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYFGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            14497..14679
                     /gene="yoaG"
                     /locus_tag="ACHKXP_003556"
     CDS             14497..14679
                     /gene="yoaG"
                     /locus_tag="ACHKXP_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO7648496.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            14827..15075
                     /gene="yeaQ"
                     /locus_tag="ACHKXP_003557"
     CDS             14827..15075
                     /gene="yeaQ"
                     /locus_tag="ACHKXP_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7648497.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(15134..15208)
                     /gene="yoaJ"
                     /locus_tag="ACHKXP_003558"
     CDS             complement(15134..15208)
                     /gene="yoaJ"
                     /locus_tag="ACHKXP_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO7648498.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(15211..15309)
                     /gene="yoaK"
                     /locus_tag="ACHKXP_003559"
     CDS             complement(15211..15309)
                     /gene="yoaK"
                     /locus_tag="ACHKXP_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO7648499.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(15342..16367)
                     /gene="dgcP"
                     /locus_tag="ACHKXP_003560"
     CDS             complement(15342..16367)
                     /gene="dgcP"
                     /locus_tag="ACHKXP_003560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO7648500.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            16550..16804
                     /gene="yoaF"
                     /locus_tag="ACHKXP_003561"
     CDS             16550..16804
                     /gene="yoaF"
                     /locus_tag="ACHKXP_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO7648501.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(16826..17173)
                     /gene="yeaO"
                     /locus_tag="ACHKXP_003562"
     CDS             complement(16826..17173)
                     /gene="yeaO"
                     /locus_tag="ACHKXP_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO7648502.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDYATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(17228..18409)
                     /gene="nimT"
                     /locus_tag="ACHKXP_003563"
     CDS             complement(17228..18409)
                     /gene="nimT"
                     /locus_tag="ACHKXP_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO7648503.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            18506..19327
                     /gene="nimR"
                     /locus_tag="ACHKXP_003564"
     CDS             18506..19327
                     /gene="nimR"
                     /locus_tag="ACHKXP_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO7648504.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGALGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIARLTTVSPQSAKPDPRQ"
     gene            complement(19284..19730)
                     /gene="yeaL"
                     /locus_tag="ACHKXP_003565"
     CDS             complement(19284..19730)
                     /gene="yeaL"
                     /locus_tag="ACHKXP_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO7648505.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            19900..20004
                     /gene="yoaI"
                     /locus_tag="ACHKXP_003566"
     CDS             19900..20004
                     /gene="yoaI"
                     /locus_tag="ACHKXP_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO7648506.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(20005..20508)
                     /gene="yeaK"
                     /locus_tag="ACHKXP_003567"
     CDS             complement(20005..20508)
                     /gene="yeaK"
                     /locus_tag="ACHKXP_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO7648507.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(20551..22041)
                     /gene="dgcJ"
                     /locus_tag="ACHKXP_003568"
     CDS             complement(20551..22041)
                     /gene="dgcJ"
                     /locus_tag="ACHKXP_003568"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO7648508.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMNAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(22222..>23457)
                     /locus_tag="ACHKXP_003569"
     CDS             complement(22222..>23457)
                     /locus_tag="ACHKXP_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7648509.1"
                     /translation="NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLIL
                     SLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYG
                     KDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
                     YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYIS
                     RGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVK
                     DFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFT
                     ELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEAD
                     KALREAKSEGGNKVIIHHI"
BASE COUNT         6079 a         5680 c         5955 g         5743 t
ORIGIN      
        1 aatttgcatc taatatcaaa gggattataa cgatatggca cgagttaacc tgttgacatt
       61 caggtatttt aaaggtagca gctcatggtg tgttatccag taagatggag aaaattaacg
      121 gtcagtccgc acgctataac cagattgttc agctaagaaa gaaaatcaga gttgctaatc
      181 aaagggcgaa cacaacggca tggttatgtt tatttatata gttagcttta tagctaaaat
      241 tgaaattata aaaataaata atgaattagc aggagcatca acagtaatat cttaatctct
      301 ggtcgattta tgtaaaaccg accagtatta tcgataaatt atcgtctgac ttattttgct
      361 tcgataaagc ttacaccgca gccacaatct taatttcaac tttatacttc gggttcatca
      421 gccccgcttg caccgtgcag cgcaccggcg catgacccgc cacaacccag gcatcccacg
      481 ctttattcat cgccgcgaag tcgtttttat cggccaggaa aatggtggca tccagaatgc
      541 tcgatttatt gctgccctgt ttttccagca cggcgtcaat ctgtgccagc gtgttggcgg
      601 tttgctcaaa ggcatcggcg tcgaggtttt ccggtacacc agtgtagtag agcgtgttgt
      661 tgtggattac tacatcagac cagcgggctt cagcatcgat acgaacgata gtcatcattt
      721 tgtcctcatt atacttccat tttcgtgctg caaagactgc cacaatgtga ggtcgccgtc
      781 accacttcaa ctggcgaaag cgccctcatc ctgacataat ccctgttcaa atcaacaggg
      841 ggtagtgtga cggacgattt tgcaccagac ggtcagctgg cgaaagcgat accaggcttt
      901 aagccgcgag aaccacagcg acagatggcg gtagccgtca cccaggcgat agaaaaaggc
      961 cagccgctgg tggtggaagc aggaaccggt acgggcaaaa cctacgctta cctggctcct
     1021 gcgctgcggg cgaaaaagaa agtcattatc tcgaccggct caaaagcgtt gcaggatcag
     1081 ctctacagcc gcgatttgcc aacagtctca aaggcattga aatatacggg caacgtggcg
     1141 ctgctgaaag ggcgctcaaa ctacctctgc ctcgaacgtc tcgaacagca ggcgctggcg
     1201 gggggcgatc tgccggtaca aatcttaagc gatgtgatcc tgctgcgctc ctggtctaat
     1261 caaacagtcg atggtgatat cagcacctgc gtcagcgtgg cggaagattc acaggcgtgg
     1321 ccgctggtca ccagcaccaa cgacaactgt cttggcagcg actgcccgat gtataaagat
     1381 tgctttgtgg tcaaagcacg taaaaaagcg atggacgccg atgtggtggt ggtaaaccat
     1441 catctctttc tggcggatat ggtggttaaa gagagtggat ttggcgaact gatcccggaa
     1501 gcggacgtca tgatcttcga cgaagcccac cagctaccgg acattgccag ccagtatttt
     1561 ggtcagtcac tctccagtcg acaactgctc gacctggcaa aagacatcac catcgcctac
     1621 cgcaccgaat taaaagacac ccagcagtta caaaagtgcg ctgatcgtct tgcccagagt
     1681 gcgcaggatt ttcgtctgca actcggtgag ccaggttatc gcggtaacct gcgtgagctg
     1741 ttagctaatc cgcaaattca gcgggcattt ttactgctcg atgacaccct ggaactttgt
     1801 tatgacgtgg cgaaactttc actggggcgt tccgccttgc tggatgcggc atttgagcgc
     1861 gccacgttgt atcgcacacg gctgaagcgg ctaaaagaga tcaatcagcc gggctacagc
     1921 tactggtacg aatgcacttc gcgccatttt actctggctc tcacgccgct cagcgtggcg
     1981 gataaattca aagagttaat ggcgcaaaag cccggtagct ggatcttcac ctcagcaacg
     2041 ctgtcggtga acgacgatct gcatcatttc acctcgcggc ttgggataga acaggcggag
     2101 tcgttgctat tgccaagccc gtttgattac agccgccagg cgttactctg tgtgccgcgc
     2161 aatctgccgc aaaccaacca gccaggttct gctcgccagt tagcggcaat gctgcgaccg
     2221 atcatcgaag ctaacaacgg tcgttgtttt atgctttgta cctcgcacgc catgatgcgc
     2281 gatctggccg agcagttccg cgctaccatg acgcttcccg ttttgttgca gggggaaacc
     2341 agtaaaggcc aactgttgca gcagtttgtc agtgccggta atgcgcttct tgtggcaacc
     2401 agtagtttct gggaaggggt ggacgtgcgt ggcgatacat tgtcattggt gattatcgac
     2461 aagttgccgt ttacctcacc ggatgacccg ctgttaaaag cgcgcatgga agattgccgt
     2521 ttgcgcggtg gcgacccgtt cgatgaagtg caactaccag atgccgtcat tactctcaaa
     2581 caaggggtag ggcgactgat tcgcgacgcc gacgatcgtg gcgtgctggt gatttgtgac
     2641 aatcggctgg tgatgcgccc ttacggcgcg acgtttctcg ccagtctgcc gcccgcgcca
     2701 cgcacccgtg acattgcccg tgcggttcgt ttccttgcga taccatcctc caggtaattt
     2761 gttacggagt atggtatgat gcgcgccttt ttattgatct aagcacgaag acccatgcga
     2821 attctggcta tcgataccgc gacagaggcc tgctctgtcg ccctgtggaa cgacggtact
     2881 gtcaacgctc attttgagct ttgccctcgt gaacatactc aacgaatctt accgatggtg
     2941 caggatatcc tgaccaccag cggaacttcc ctgactgata ttaacgctct ggcttacggg
     3001 cgcggccccg gtagctttac tggcgtgcgc attggtattg gcatcgcgca agggctggcg
     3061 cttggcgcgg aattaccgat gattggcgtc tccacgctaa tgacgatggc gcaaggggcg
     3121 tggcgcaaaa acggcgcaac ccgtgtgctg gcagccattg acgcgcgaat gggcgaagtt
     3181 tactgggccg aatatcagcg tgatgaaaac ggtatctggc acggtgaaga aaccgaagcc
     3241 gtactcaaac ccgaaatcgt ccatgaacga atgcaacagc tttccggcga atgggtgacg
     3301 gtagggacgg gctggcaagc ttggccggat ctcggtaaag agagcgggct ggttttgcgc
     3361 gatggcgaag tgttactgcc tgctgctgaa gatatgctgc cgattgcgtg tcagatgttt
     3421 gccgagggta aaacggtggc ggtggaacat gccgaaccgg tttatttacg taacaacgtc
     3481 gcatggaaga aacttccggg caaagaatga atcttaagag tatgccctga gaaaaaaagg
     3541 agtcgcacca tggcggttca aaagaatgtt atcaaaggca tactggcagg tacgtttgcg
     3601 ctaatgctga gcggttgtgt cactgtgccg gacgccatta aaggcagcag tcccacgccg
     3661 caacaagatt tagttcgggt gatgagtgcg ccgcagctgt acgttggtca ggaggcacgc
     3721 tttggtggca aagtggttgc ggtacaaaac cagcaaggga aaacccgcct ggaaattgct
     3781 accgtaccgc tggacagcgg agccagaccg acgctgggag aaccttctcg cggtcgcatt
     3841 tatgccgatg tgaacggttt tctggacccg gtggatttcc gtggacaact ggttacggta
     3901 gtcgggccaa tcactggtgc ggttgacgga aaaatcggca acacgcccta taaatttatg
     3961 gtgatgcaag taacgggtta caaacgttgg catttaaccc agcaggtgat tatgccgcct
     4021 cagccgattg atccatggtt ttatggcggt cgtggctggc cctatggcta cggcggatgg
     4081 ggctggtata atcccggccc cgcgagagta caaacagttg taactgaata attgcttgtt
     4141 tttaaagaaa aagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
     4201 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaatct ggtatgatga
     4261 gttaatatta tgttaacggc atgtatatca tttggggttg cgatgacgac gaacacgcat
     4321 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
     4381 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
     4441 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
     4501 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
     4561 cgcgttgcgt tgatgatgcc taatttattg caatatccgg tggcgctgtt tggcattttg
     4621 cgtgccggga tgatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
     4681 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc tcacacactg
     4741 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
     4801 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgtctggtg
     4861 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
     4921 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
     4981 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
     5041 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
     5101 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
     5161 ggtgggcaga acctgcttat cactaacccg cgcgatattc cagggttggt aaaagagtta
     5221 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
     5281 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
     5341 ccagtgcagc aagtggtggc agagcgttgg gtgaaactga ccggacagta tctgctggaa
     5401 ggctatggcc ttaccgagtg tgcgccgctg gtcagcgtta acccatatga tattgattat
     5461 catagtggta gcatcggttt gccggtgccg tcgacggaag ccaaactggt ggatgatgat
     5521 gataatgaag taccaccagg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
     5581 ctgggttact ggcagcgtcc cgatgctacc gatgaaatca tcaaaaatgg ctggttacac
     5641 accggcgaca tcgcggtaat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
     5701 gacatgattc tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
     5761 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
     5821 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
     5881 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
     5941 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
     6001 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
     6061 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
     6121 gcgctggctt ctttgtgtga agccgtccgt gcctttccgg cgatagccct ggatactgaa
     6181 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
     6241 catctggcgc taatcgatcc actcgggatc accgactggt caccgctgaa agcgatcctg
     6301 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
     6361 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
     6421 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
     6481 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
     6541 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
     6601 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
     6661 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcaattacgc
     6721 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
     6781 cgcgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt agcgcgttat
     6841 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
     6901 cacggtaaaa cgctgctagc gctggtggaa aaagcgcaga cattgccgga agatgcctta
     6961 ccgcagccga tgcttaacct gatggacatg ccgggttatc gtaaagcgtt taaagcgatt
     7021 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
     7081 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
     7141 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
     7201 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
     7261 gcttcggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
     7321 aacatacttt gctggctggc acgctcttca tcgctaaaat attgatcccg atggtcagct
     7381 tcaccttcca gtattgctgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
     7441 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
     7501 aactcttttc cggaacgggc aagcaccagg gtaaaggcat cgccggtttt gtcttcgata
     7561 gcaaattgct caaagtgcag cgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
     7621 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
     7681 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg agtgatgcaa gtgaactttc
     7741 tcagcctgcg gatgctggac tagctcatca cgaaatgcgc aactttctgg atttcgcgag
     7801 cagtaatgta gctgccagtc gacgtcgctg tgttgcagct ctgccatgtg cgacaggaaa
     7861 ggggtgatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
     7921 aacaggttat taggcgttga aatcgttaac cgattgccca cttttacctg ctgatgcaaa
     7981 aagcgggaac cgccgcgcga gttttcctcc agccgaacgg caatctgata acaagaggtg
     8041 tcatgcggcg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
     8101 tgcacaatga cgtgacttcc tccggtaaac gcaggtaatg gtttgccatc ggttgccacc
     8161 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
     8221 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
     8281 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
     8341 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
     8401 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gacggaacac
     8461 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
     8521 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
     8581 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
     8641 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
     8701 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
     8761 gaaacctgga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
     8821 ccagttggca ggcgtgcggg tggtaaagcg ggccgccagt ttcagatcgt ggacttccgg
     8881 gcaggcttcc agcactttcg cgcccaggcc gggtaattga tcttctacgc tggtggcgtt
     8941 ggggtccatg ttgatgaaga cgaatccggc atattcttcc agacgcaccg gaaccagttg
     9001 cgctttgtcg ctatcgaaat tggcgacgtt ttcgcagtta cgtgcatggg ccaggttgcc
     9061 atcgagtttg aatgcccatg cgtgatacgg gcaggtaatc acattttttg cttttccttc
     9121 accgctcaac aactgatgac cacggtgcgg acacacgtta tagaacgcgc gcaaaacctt
     9181 atcacgaccg cgtaccagca cgatgctttc gccaatgatc tcacgcgtca cataatcatt
     9241 ggcattcgcc agttcgctgc tgtgagcgac gcaaatccag cttttggcga agacgttctc
     9301 tttttcgtgt tcaaacgcgt tctgatcggt ataaaaacgg gcaggaatgg tccacgcctc
     9361 ttgcgggtta gcgcaaaaat tttcgggtag tacaaagtca gggctcagat tgctcatagg
     9421 gataccttta tcgttaggat gatggcgctc agttgtcgcc tttgaatacg gttcccgcag
     9481 ggagcactgg cgttttttcc agggcttcaa ccggtgtctg cgggagataa tgttcaactt
     9541 gatgagccgg aatgtcggcg taatcctgtt tcagccagcg aataaacccg ccgactttca
     9601 ccagtaaaat gacgaggaag ggaagggctg tgagcacgac ggtggttttc atcgtttcca
     9661 gcgaagcacc ggtaaacaag atggaaagcg ggatcagagt gatcaccacg caccagaaaa
     9721 gacgcagccc acggtcagga tcgtcacctt cctggagatt acgcgtactg gtcgccgcca
     9781 tggtgtaggc cactgcatcc atatgcgagg caaggaaaat aatcatcacg cccaggtatg
     9841 cggcgaggaa caatttaccg gctggcaacg acatcagaac ttgctgtaca gctgtttcgc
     9901 cgcccagtgt ttccagcacc tgtgggacgt tgattacgcc attgataaac tgatgaatgg
     9961 catagctttc cattacgcca aagaagaacc agcaaccgac ggtgctgccg aggatcagtc
    10021 cccagataac ttctttaatc ttacgaccgc gggaaacgcg ggtgacaaac attgctacgc
    10081 caggggtgta tgagatccac cacagccagt agaaaacggt ccagttgcgg gtaaatgaac
    10141 catcgccaag cggatcggtg aataagctca tttgcaggaa gttttgcgtg gtcaggccga
    10201 tggcattgat gatgctgttg gtaataaatt cggttgggcc gacaatcagc accagtaatg
    10261 gcagcaggaa cgcgccccag ccaaccattt tgctcagacg ttgcaaaccg ttgttgatac
    10321 caatccacga gcttaggcaa aaaatgccgc cggaaagcag gatcacaaat gcctgcacgg
    10381 tgaagttatc gggtaaaccg gtcagcgcgg aaagcccacg ggtaaaggtt gctgcggtaa
    10441 caacaaggga aatggtcagt gcgccgacag tggcgatcag gaacatcaaa tcgaccagtt
    10501 ttccccatgg gccttgcggg cgaacgccgg taatagcagc aataatgccg gaaaggctca
    10561 gacctttgtt tttccgcaca tgaaagtgat aagccatgat taatgaggcc agcgtataag
    10621 ttgcccaggc gctgatgccc cagtggaaga aagagtaggg aacgctaaat tcgagtgcct
    10681 gttgtgaacg cggtgcgata tttaatccag gcgtttgata ataataggcc cattcagcaa
    10741 ccccccaata aagcgtagaa gaacctaaac cggcacaaat aaacataaac agccaggaga
    10801 gcgtgctgta ttccggtttt ccttcgccaa gacgaatatt gccgtattta ctggtggcta
    10861 aataaatcac cagtcccatt gccagcaaaa ccaatacctg tacggcggaa cctaacgtac
    10921 gcgtaacaaa atttgcatgt tacataaatt aaacaattaa gaatacggaa agccataaaa
    10981 cacgaaacgg aaacaataaa aggtgaaatg acgcaggtta tttactgcaa agaacgaata
    11041 aaaaaaccgc gtcagtgacg cggttcaaat aaactggttt gaccttaacg caaaataatt
    11101 ttgcaaatcg cgtcggcaac ctgtggcgtg gtggcattgc ctttcatatc aggtgttttc
    11161 ggcccgtgag caatcacttc ttcaattgct gccagaatac cgttatgcgc ttgctggaaa
    11221 cgctcatcgc cattgccgag aaaatcgagc atcattgccc cggcccaaat cgtggcgata
    11281 gggttagcaa tatttttccc gtagatatcc ggcgcggaac cgtggacagg ctcgaagagc
    11341 gacgggaaag tgcgttccgg attcaggttg gcggatgggg caatgccaat ggtgccggtg
    11401 caggccgggc caagatcgga aaggatatcg ccaaacaaat tggacgccac caccacatcg
    11461 aatcgttccg gctgcatcac aaaacgcgcg cagagaatat caatatgctg cttgtcccag
    11521 cggatctccg ggtaattctc ggccattgct tccactcgct catcccagta cggcatgctg
    11581 atggctaaac cgttcgattt agtggcagaa gttagcgtct tacgtgggcg gctttgcgca
    11641 agttcgaagg cataacgcaa aatgcgatcg acaccacgac gcgtaaatac cgattcctga
    11701 atgacgactt catgctctgt accttcattc actctaccgc cgagcgagga atattcgcct
    11761 tcggtgtttt ccctgaccac gtaaaaatcg atgtcgccag gctgttttcc cgccagcggg
    11821 cagggaacgc caggaaagag acgaaccggg cgcaggttga cgtactggtc gaattcacga
    11881 cgaaatttca gcagcgaacc ccacaacgaa atatggtccg gaacggtatc cggccagccg
    11941 acggcaccaa aatagatggc gtcgaaacgg ctaagttgct catgccagtc gtccggcatc
    12001 attttaccgt gatggctgta atactcgcag ctcgcccact ccatttgctc aaaactcaag
    12061 gcgaagcccc agcgctcagc ggcagcctgt aacacgcgaa tcccttcagg aaggacttct
    12121 ttgccaatcc cgtctcccgg gatcgcagca atacgcatcg ttttcatcat tgggttctca
    12181 cttcctgacc atcaaaattg acaacatcac catcctataa ttgactgaaa aggaattaat
    12241 ccccgtaact ggtgaaacat aaaacacgaa tcatgaataa tttaccgctg ctgaatgatt
    12301 tgcgcgtctt tatgctggtg gctcgccggg ctggttttgc cgccgtggcg gaagaactgg
    12361 gcgtttcacc ggcgttcgtc agcaagcgca tcgccttgct ggagcaaacg ctaaacgtgg
    12421 tgttgttaca ccgcaccacc cgccgcgtca ccattaccga agaaggagag cggatttatg
    12481 aatgggcgca gcggattttg caggatgtcg gtcagatgat ggacgaactt tctgacgtgc
    12541 gtcaggtgcc gcaggggatg ctgcgtatta tcagcagctt tggttttgga cgacaggtgg
    12601 tggctccggc gttatcagcg ctggcgaagg cgtattcgca actggaatta cgttttgatg
    12661 tggaagaccg actggtcgat ttggtcaatg aaggtgtcga tctcgatatt cgcattggcg
    12721 atgacatcgc ccctaattta atcgcccgca aactggcgac caattaccgt attctctgcg
    12781 cgtcgccgga atttattgcg caacatggcg cgccgaaaca tttaaccgat ctttctgcat
    12841 tgccgtgcct ggtcattaaa gagcgcgatc atccttttgg tgtctggcaa ttacgcaata
    12901 aagaaggccc ccatgcgatt aaagtcaccg gcccactgtc gtctaatcat ggagaaattg
    12961 tccatcagtg gtgtctcgat gggcagggga ttgctctgcg ttcctggtgg gatgttagcg
    13021 aaaacattgc cagtggtcat ttagtgcaag tgctaccgga atattaccag ccagcgaacg
    13081 tctgggccgt ttatgtttca aggctggcga cgtcagcgaa agtgcggata acggtagaat
    13141 ttttacgcca gtattttgcc gagcactacc cgaatttttc actggagcat gcctgattaa
    13201 tgattcaatt atcgggttga tatcaggtta aaacctgatt ttctcctttc taagccgcta
    13261 cagattggtt agcatattca cctttaatcg cgcatgatcg aaagataatt aaagaggtta
    13321 atgtgttcgc tgaatacggg gttctgaatt actggaccta tctggttggg gccattttta
    13381 ttgtgttggt gccagggcca aataccctgt ttgtactcaa aaatagcgtc agtagcggta
    13441 tgaaaggcgg ttatcttgcg gcctgcggtg tatttattgg cgatgcggta ttgatgtttc
    13501 tggcatgggc tggagtggcg acattaatta agaccacccc gatattattc aacattgtac
    13561 gttatcttgg tgcgttttat ttgctctatc tggggagtaa aattctttac gcgaccctga
    13621 agggtaaaaa tagcgaggcc aaatccgatg agccccaata cggtgctatt tttaaacgcg
    13681 cgttaatttt gagcctgact aatccgaaag ccattttgtt ctatgtgtcg tttttcgtac
    13741 agtttatcga tgttaatgcc ccacatacgg gaatttcatt ctttattctg gcggcgacgc
    13801 tggaactggt gagtttctgc tatttgagct tcctgattat atctggtgct tttgtcacgc
    13861 agtacatacg taccaaaaag aaactggcta aagttggcaa ctcactgatt ggtttgatgt
    13921 tcgtgggttt cgctgcccga ctggcgacgc tgcaatcctg atgctttcag cccgcgttgt
    13981 cgcgggcttc ccatctataa tcctccctga ttcttcgctg atatggtgct aaaaagtaac
    14041 caataaatgg tatttaaaat gcaaattatc aggcgtaccc tgaaacggct ggaataaacc
    14101 gttttcagcg cattcaccga aggagggaaa aggatgcttc aaatcccaca gaattatatt
    14161 catacgcgct caacgccttt ctggaataaa caaactgcac ctgccggaat attcgaacgt
    14221 catcttgata aaggaacgcg cccgggggtt tacccacgcc tttccgttat gcatggggcg
    14281 gtcaaatatt tcggctaccc tgatgaacac agtgcagagc ctgatcaggt gatccttatc
    14341 gaagcggggc agtttgcggt gttccctcca gaaaagtggc acaacattga agccatgact
    14401 gacgatactt atttcaacat tgacttcttc gtggctcctg aagtcctgat ggaaggtgcg
    14461 caacaacgga aagtcattca taacgggaaa tgaatcatgg gcaaagcaac gtataccgtg
    14521 accgtcacca ataacagcaa tggcgtttct gtcgattatg aaacagagac gccgatgact
    14581 ttgctggtgc cagaagtggc ggctgaagtg ataaaagatc tggtgaatac cgtacgttct
    14641 tatgacacgg aaaacgaaca tgatgtttgt ggttggtaat tatcactgat cgttgatggc
    14701 gcaatttctg cgccatcgca aaaaaaacct ccgtgatgct cgtcacaaat cttgccctcc
    14761 cgatgttgtg ctatgctttt tatcagcgac taacccgcta atgcaaaaca gataagtgag
    14821 gaaacaatgg gcattctgtc atggattatt tttgggctta ttgccggtat tctggcgaag
    14881 tggatcatgc caggtaaaga tggcggtgga ttctttatga ctatcctgct ggggatagtc
    14941 ggtgccgtag tcggcggatg gatcagcacg ctgtttggct ttggtaaagt cgatggcttc
    15001 aattttggca gcttcgtggt tgccgttatt ggtgcgattg tcgtgctatt tatctacagg
    15061 aagattaaaa gttaacgctt aaattgcaca aaggctgcac acaggcagcc tttgctattt
    15121 tttagaggtg acgttaccac catcccagct cagtgccgag tacgacaata atcgccacac
    15181 ccatcataat aatcgttgtt tttttcatct ttatgctccc ggggcagcat agccgccaat
    15241 aaaaaaccat gctaaaaata cgaccgctgt aatgaagatt acaaccggaa aaataatacc
    15301 gattcgcatg ttatcaccaa tcaaaaggat gtgaatacgc ctcaggaatg tagcgctgga
    15361 tgcgcgacaa aaggtgtttt ctgtttgtgt tttttctcct gatacatcgc aatatcggca
    15421 gcatgcaggg cactgtctgc atcggttgtt tcaggatcga cttctactat gccaagactg
    15481 gcaccgggat aatacaaatc aacatcacct aagtgatatt ctccacgtat ttgctgctga
    15541 atgcgttctc gcagcgacga aatatccgca ttctcgttgt ttagtgaaac aactaaaaac
    15601 tcatcaccgc cgagacgacc aataacttcg ccattttgct ggagcgtatt aaggcgctcg
    15661 ccaacctgaa tgagaaacag atcgccacta ttatgaccaa aacgatcatt gattaatttg
    15721 aagttatcca gatcgataaa cgcgatcatt atcttatggt taagatgccg ggcgagggaa
    15781 aacagtgtcg tcagattttc aaaaatcgcc cgccgattcg gtagcccggt taacgagtcg
    15841 gtatacgatt gcgcaatcag cgcagcattg gcttcgcgca gctgttcaac cagtgcctct
    15901 ttttgaatat attgtgcaat cagtccggcg aataactgta aaacctgttc cgcgcgttca
    15961 ctccactggc gcttctcact gctggcggcg caaagcgtgc catagaatga tccatccggt
    16021 aagtgaattg gcgtgctcag aaatgtggtg atgccaagat tgcgtgccgc aatacagtca
    16081 ccccaacggt cggggacttc atcgctgaaa aagcagtttt catcaatggc gcgtttgcat
    16141 aacgagtaat cccacgagac ggtaaaattc tccgggatgt gcattttctg gctgttacgg
    16201 gcaaacataa tatgctgcag gcgcgcttcg acatccactt tggtcaggta ggttgattcc
    16261 atatcagtga ccatttccag catctccaga agctgtcgga ccagactttc cagtgactgt
    16321 tctttggcaa gggattgcga gacgcgggcg ataatctgat ctgacacgga acaactccag
    16381 gatacggcgg gtaaatgctg attatttaat ctttgcaggc atgataaaat atcatcaaca
    16441 aagatgaaac aagacgtaat atgttttgag tgaatgtaat aaaaatagtg ctgcaaaaca
    16501 aaacgatacc gcgatactct aatcttggat aaatatgtga gatcaataaa tgaaaattat
    16561 cagttttgtt ctgccttgct tgctggtcct ggctggttgt tcaacccctt ctcagccaga
    16621 agcacctaaa ccgccgcaga ttggtatggc aaatccggcg tcggtctatt gccagcagaa
    16681 gggcgggacg ctcattccag tgcagacagc gcaaggggtc agcaacaatt gcaaattacc
    16741 gggcggtgaa acgattgatg aatgggcatt gtggcggcgt gaccatccgg ctggtgaaaa
    16801 ataaccgccg gatgctgtac taaaatcaca agctacgtag ccagtcggcc agcaccagcg
    16861 catggttctg cgtggtgttt tttgctgagt agagcagggt cagcggctgt tttttggcga
    16921 tgtccgccag ccgctttcct tcttgctcgt gttgcgccag ttctgcaaga tattgctcgc
    16981 gaaaggttgc ataatcgacg acttcgccgt gaaaggcttt gcgcagttcc gttgacggcg
    17041 tgatttcttt atcccactca tcaagggcta aatcggtttt tttgataccg cgcggccaga
    17101 ggcggtcgac cagtatgcga taaccatcgc tctgttcagc cggatcataa acgcgtttgc
    17161 actgaatatt catcgtgttt gttctccctt tcacgcatga tttgttacgt cactatagca
    17221 ccggatatta gcgaatttct ttgtctctcc cggcgcaaag tccgaaaatc gccatcagta
    17281 gtgaaagtat ggcaacaccc ataagtggta cagaccagtt accgttagca tcgtgtattt
    17341 tacccatcag cggcggccca caggctgcca acaaataccc gacggattgt gccatccccg
    17401 agagtgccgc cgcctgatgc gcagaactag cccgcagacc aatgaacgtc aaccccagta
    17461 tcattgttgc gccggaacca aaaccgaaaa gcagagtcca ggtgatcgcg tgcgctggca
    17521 taaagcagag cccaaccgcg cccactgcgc acatcaaggc aacgaacgct gcaataccac
    17581 gctgatcttt cacatgatgt aagaaaagtg ggatcagcaa accgggtgct gctgtggcta
    17641 gttgcagcaa accatgcagt gaacccgcct gtgcttcgct atagccgtga ctgatgagga
    17701 tcgccggaag ccagccaata atcacgtaat agaccagtga gttgatccca agaaacaatg
    17761 tgacctgcca ggcaagcggc gaacgccaga taccccgagt atgtaaggcg cgcgaggtac
    17821 tcaaatttgc atgttgttga cttcgccact gtggcagcca taaaaaaaga gccagcagag
    17881 gaaaacacat cagcatgagc aacgcgcctt gccagccaaa accgttcaaa gccagcggca
    17941 cgaccatagc cgatcccagt gccgctgcag ctcccattgt cagggaatat gcgccggtaa
    18001 gtctggcgac ggaatgaggg aaatcgcgtt taattaatcc tggcagtaag acattgccta
    18061 atgcaatccc accgccaatg accgctgtac cgccaaataa taagtaaggc gaagggagag
    18121 agcgaattgc gataccagca cagatcaaaa gtaacgcggc aaacaggcta cgttccatac
    18181 caaaacgtcg cgctaccggg gcagccaaag gtgagattag cgcaaaggcc aataatggca
    18241 gggtggtcaa taagccggtt tgcgctgtcg tcagcgagta agcggaacga atcgtatcca
    18301 gtaacggtgc tgcgccggta aaggtgacgc gtaatgttgt ggcaatcatc agaatgccag
    18361 cgataaggac aatcctgttt ttgccgctta atgaagttga acaggtcata tgcttctcat
    18421 tgttacaaac gaggctgtac cttaccgttt ttattgttat gttgaatcaa gctaaaatga
    18481 caaattatcg ctaagttcgg acaaaatgat gcacaggttg aatctcaatg gctatgaacc
    18541 cgaccgacac catgaagccg cggtcgcatt ttgtattcac gccggaacgg atgagttaac
    18601 cagtcctgta catcagcacc gcaaaggaca gctgatactg gcgctacatg gtgcaattac
    18661 ctgtacggtg gaaaatgctt tgtggatggt gccgccacaa tatgcggttt ggataccggg
    18721 cggggtggaa catagcaatc aagtaacagc taatgctgaa ctctgttttc tgtttatcga
    18781 acccagcgca gtgacaatgc cgacaacatg ttgcacattg aaaatatcgc ccttatgccg
    18841 ggaattaatt ttaaccctgg caaacaggac gacgactcag cgtgctgagc caatgacccg
    18901 cagattaatt caggtcctgt ttgatgaact ccctcagcag ccacaacaac agctgcacct
    18961 gcccgtttct tctcatccta aaatccgcac gatggtggag atgatggcga aagggcctgt
    19021 cgagtggggg gcattggggc aatgggctgg cttttttgcg atgagtgaac gcaatcttgc
    19081 ccgactgata gtaaaagaga cggggttgag ttttcgccag tggcgtcagc agttacagct
    19141 gattatggcg cttcagggat tagtgaaagg cgatactgta cagaaagttg ctcatacgct
    19201 ggggtatgat tcaacaacgg cctttattac tatgtttaag aaaggcctgg ggcaaacgcc
    19261 aggccgatat attgcgagat taactactgt ttccccacaa tcagcgaaac cagacccgcg
    19321 gcaataagcg gcccaaccgg tacgccgcga aacagcgcta cgcctaaaac agtgcctacc
    19381 agcagcccgg cgaccagttg cggctggctg cccattaacg tcacgccgcg cccacccagc
    19441 caggagacaa tcacgcctac tgcaatcgcc accagtgatt tccagttcag aaacgagtga
    19501 atcaaggttg agggcggtag cgtcccgctg gcgatggggg ccataacgcc aatcgtcaga
    19561 atgattatcc cgatacttag cccctgtttt tcaatccacg gaaaaaaggt gcttaacggt
    19621 gtgacgcgga caatgattaa caccagaatt gaaacggcga cagtggtgtt atgactgata
    19681 aagcccagcg cagctaaccc gagcaggatc agcaaagtga catcaaacat cgtggtttcc
    19741 ttgccaaaaa ataataagcc tgcttacttt acgcttgctg caggaaggaa gtagaaaatc
    19801 tttacgtttt ttgcataaaa aatcaggcga actgtcatca aactgccatt tgtgcacgct
    19861 aacaataacg tgaaaacagc tatctcccgg gggcatgcta tgaacgatca aatgtttgtc
    19921 gagacactga ttatcacgtc atcgtttttt gccattgctg ttgtactggt tttgtccgtt
    19981 cttcttatcg aacggaccgg ctagttacgc agtgcggcgg aaattgacca gttctggttg
    20041 cgcaatgcgc aaataatccg cggttttcaa aataacggat ttatccagca tgcctgcatt
    20101 gaaggcgatt tcatcaaatc gttcgaagag tagagggtcg gcaaccagtt tgagttttgg
    20161 atggaagctg aaagggggga tcgcgccgaa gacacagcct gtgagttcat cgacttccgc
    20221 cgggctggcc aacgaagcgc gtaatccgcc tatatggctg gcgagttggc tcagatcggc
    20281 ctgttgatcg gctgcgagga tcgccagaac atgttgattt acgccattgc ctttgacttt
    20341 acataccagt gccttagcac cctggcctag agcggtgcca cgaatttctg acaccgcttc
    20401 acatttacct accgcttcat gggtaacgac gcggaagtca gcaccttctt gtgataataa
    20461 agcgattaac cgctgatgcg tcacgcttcc tttagccatt tcagtcattt cacgctcctg
    20521 agattacaag caaacaacca cagaaggtta tcatgaacgg ctgtttttgt tctgcttatt
    20581 gacatataaa cgctcatcgg aagctttata cgcatcatgt aacgtatcgt tttctttcat
    20641 cgcgtaaata ccggaagaga agccgatctc tttctgcggc gcgatatgtt gcagacgttt
    20701 ttcgatacgt tcaggcagtt gtgctgcaat ttgcggcgtc gaatcgacaa gaatgatgca
    20761 gaattcatcg ccaccgagtc ggatggcata atcactttta cgaatcgact gtttaatcgc
    20821 ctgagctaat aacgtaatcg ctaaatcccc ctcctgatga ccgagggtgt catttatttg
    20881 ctttaacttg tccatgtcaa tagcaataaa catcaccgaa gaaccggatt gcaccagttt
    20941 ctgcaaccgc tgctccagtt caggggttaa aattttgcga ttatacagtc cagtcatcgc
    21001 atcactaata ttttctcgcg acacattttg gtataaacgg aagtgcatcc gcaccatatt
    21061 cagcaaaatt aacgccgtca aaatccagaa cagaacgctt ttccacgaag acgtgataaa
    21121 gtaaagaata tcaatggata acgagacacg aatgccgccc ggtaagtcat ggacgtaact
    21181 gacatactgg aacagattat cttcgctctg gttgatgata atgtcgcgcc ccgaatcggt
    21241 atcggttagc gtgacattga gaaaacgcca gaggagaggg cggtcatgag tataaaagat
    21301 attccgcaga ttgtttttgt taatatccag cagcacaatc ccttttagct gccctgcgac
    21361 atataccggc gtcagcaaac tcatcacgtt ctgtttagta cgctgatcct ggtaaatgct
    21421 cgacaatacg gtacgcccgg taaatacctt ttcaatatct ttgcgatcga tgccaatact
    21481 tcctgactgc aggaaggacc agttattcat cgcaaagtat tcaacattaa ccggctcata
    21541 aaaatagaca tagcggtcat tgagatcgag ataatagtga aatttctctt cggccatccc
    21601 aagacctgaa ccgtaatcat cctggtggcg gttgccttcc actgcctgat caaaagcggg
    21661 taatagtgct gcagcttcca caattgtgtc gcagtcagtg gatgcggttt gtagtgtgcc
    21721 gcgtaagccc gggtatttat gggcagtcag gtttaagcca tgggtgttat tggcagactc
    21781 aaaagcctcg cagatggcgc gccgctgttc tggcgacact tccgactgct cagcagcgag
    21841 agcgcgcatc acatgcgcgg caatactctg attctgatac ttatcataaa gaaaggagga
    21901 gtctgctttc tgcacgatat agcgcagata ggcattcatt gctctgtcgc tggcgacaag
    21961 ttcaaaaata aggaaggaag atgtcagcac aatgacactt gcggcgataa aatgccggag
    22021 cattctatgg tgcaatttca tgataaatgg cactccttcg gttagtgaaa cgagaaaacc
    22081 agataaagtt cgattgcggc ctggcacagg ggcgctaatt atactcatct ctgcgccgct
    22141 ggggagggga aatttagggg taatagtaat aaattgtatg caaaagccac gcaaaaagca
    22201 tggctctgaa tatttttgca cttaaatatg atgaataatc actttgttgc ccccctcgct
    22261 tttcgcttcg cgtaaggctt tatctgcttc cgtcatgaca agcgagatat tacgcgtgtc
    22321 acccgttgaa aaaaccgtgc caatactgat ggtcatttgt tcaggaacat catatctgtt
    22381 actaaagccg gtcaggagtt cgacattttt acgcatacgt tccgcaatga tttttgcctg
    22441 ggcactattg agttcggtaa atagcaagcc aaacacctca ccctccagtc gcgctaaaat
    22501 atcatcgggg cggatgcttt gctggataat atcgacaatt gaaaccagca ctttatcacc
    22561 cacacgatgt ccccattggg cattgatgcg tttgaactga ttaatatcaa ccagcatgac
    22621 acagaagtcc ttaacaacag gtcgcgacaa taatgactct acgctgttga aaaagtagcg
    22681 ccgattataa atattggtca gcacatcatg aactgccagt ttgctggaga gttgcagctc
    22741 ctgaaaaatg ttataaatca gaaaagacac aacaaacagt ttactggaaa cttcaatccc
    22801 gcgactgata taccagatag tataattata ctcatccaga gtcagtagaa gtaagttgca
    22861 gcatagcgat gccagacata ataatgctat taacggccat agttcgctgg ctaatcgtgt
    22921 gcgcatgata ataaagaaca gtaaaaatgc ccacagacaa accaatattt ttgtatagtt
    22981 gattccccag gtcgcagtat ggttgtccgg acagtaatcg acgacataca aagaatagtc
    23041 agcgttataa ctgctcagat tgtgtgccag aaggggaaaa actaaaaaag ggatcagcgc
    23101 cagcaacagg attcccgttt ttttcttatt gttgtcaagg atgttgtctt ttccataaca
    23161 aaagagcgcc agcgaggtta aacaaataaa actgagttgg cggaaaatat agaaaataga
    23221 aacatcattc gactttgtct ggatcagtgt actgccgtta attggtttat ggataattac
    23281 aatggtttca actaaataga taaggccact taaaaatgca aggcttaaaa tgagtgttgg
    23341 taaaatttgt ttgttagtga aatatttcat taacatgaaa agaaaaatga cggcgttaag
    23401 gaaaaacaaa gccacaacaa ccatcaggta attcatctgt gggatgagtt ttacatt
//
LOCUS       DAZFZC010000081        23419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFZC010000081 DAZFZC010000000
VERSION     DAZFZC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.081"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..102)
                     /gene="cbtA"
                     /locus_tag="ACHKXP_003570"
     CDS             complement(<1..102)
                     /gene="cbtA"
                     /locus_tag="ACHKXP_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO7648510.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHY"
     gene            complement(191..559)
                     /gene="cbeA"
                     /locus_tag="ACHKXP_003571"
     CDS             complement(191..559)
                     /gene="cbeA"
                     /locus_tag="ACHKXP_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO7648511.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(633..854)
                     /locus_tag="ACHKXP_003572"
     CDS             complement(633..854)
                     /locus_tag="ACHKXP_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7648512.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(917..1393)
                     /locus_tag="ACHKXP_003573"
     CDS             complement(917..1393)
                     /locus_tag="ACHKXP_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7648513.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(1409..1888)
                     /locus_tag="ACHKXP_003574"
     CDS             complement(1409..1888)
                     /locus_tag="ACHKXP_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7648514.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(1970..2791)
                     /locus_tag="ACHKXP_003575"
     CDS             complement(1970..2791)
                     /locus_tag="ACHKXP_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7648515.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(3012..3422)
                     /locus_tag="ACHKXP_003576"
     CDS             complement(3012..3422)
                     /locus_tag="ACHKXP_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648516.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(3438..4121)
                     /locus_tag="ACHKXP_003577"
     CDS             complement(3438..4121)
                     /locus_tag="ACHKXP_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648517.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            complement(4257..5327)
                     /locus_tag="ACHKXP_003578"
     CDS             complement(4257..5327)
                     /locus_tag="ACHKXP_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO7648518.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(5324..6229)
                     /locus_tag="ACHKXP_003579"
     CDS             complement(5324..6229)
                     /locus_tag="ACHKXP_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO7648519.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(6226..8622)
                     /locus_tag="ACHKXP_003580"
     CDS             complement(6226..8622)
                     /locus_tag="ACHKXP_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO7648520.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            complement(8840..9712)
                     /locus_tag="ACHKXP_003581"
     CDS             complement(8840..9712)
                     /locus_tag="ACHKXP_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7648521.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGPREVQRFRLNGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILLELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVMQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHRVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            complement(9796..10947)
                     /locus_tag="ACHKXP_003582"
                     /pseudo
     CDS             complement(9796..10947)
                     /locus_tag="ACHKXP_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12215..12484)
                     /locus_tag="ACHKXP_003583"
                     /pseudo
     CDS             complement(12215..12484)
                     /locus_tag="ACHKXP_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            12570..13592
                     /gene="istA"
                     /locus_tag="ACHKXP_003584"
     CDS             12570..13592
                     /gene="istA"
                     /locus_tag="ACHKXP_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFO7648522.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            13589..14371
                     /gene="istB"
                     /locus_tag="ACHKXP_003585"
     CDS             13589..14371
                     /gene="istB"
                     /locus_tag="ACHKXP_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFO7648523.1"
                     /translation="MMMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            14410..14562
                     /locus_tag="ACHKXP_003586"
     CDS             14410..14562
                     /locus_tag="ACHKXP_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648524.1"
                     /translation="MDHFSSVVDICGQSFWLAVQGMFSGYRVPQSRDIAPIVTFQIDR
                     GLKMWH"
     gene            complement(15300..15902)
                     /locus_tag="ACHKXP_003587"
     CDS             complement(15300..15902)
                     /locus_tag="ACHKXP_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648525.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(15996..16202)
                     /locus_tag="ACHKXP_003588"
     CDS             complement(15996..16202)
                     /locus_tag="ACHKXP_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7648526.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSHP"
     gene            16943..17086
                     /locus_tag="ACHKXP_003589"
                     /pseudo
     CDS             16943..17086
                     /locus_tag="ACHKXP_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(17430..17626)
                     /locus_tag="ACHKXP_003590"
                     /pseudo
     CDS             complement(17430..17626)
                     /locus_tag="ACHKXP_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17802..18371
                     /locus_tag="ACHKXP_003591"
     CDS             17802..18371
                     /locus_tag="ACHKXP_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7648527.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKVHCTGERNFGCSRG"
     gene            18537..18929
                     /locus_tag="ACHKXP_003592"
     CDS             18537..18929
                     /locus_tag="ACHKXP_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7648528.1"
                     /translation="MYAKSFLALDSNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSLPRTAQQLS"
     gene            complement(18939..19907)
                     /locus_tag="ACHKXP_003593"
                     /pseudo
     CDS             complement(18939..19907)
                     /locus_tag="ACHKXP_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            20093..20497
                     /locus_tag="ACHKXP_003594"
     CDS             20093..20497
                     /locus_tag="ACHKXP_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO7648529.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRERGAAGGQLEG"
     gene            20788..22406
                     /locus_tag="ACHKXP_003595"
                     /pseudo
     CDS             20788..22406
                     /locus_tag="ACHKXP_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            22486..22778
                     /locus_tag="ACHKXP_003596"
                     /pseudo
     CDS             22486..22778
                     /locus_tag="ACHKXP_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(22818..23419)
                     /locus_tag="ACHKXP_003597"
                     /pseudo
     CDS             complement(22818..23419)
                     /locus_tag="ACHKXP_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
BASE COUNT         5529 a         5990 c         6042 g         5858 t
ORIGIN      
        1 atagtgctgg tccagcagtc gggacagcag tatctgccag atttcaacag gagacgggcg
       61 agaactggcc gcctgcccgg gtaatacagg taatgttttc atactgaaga ttttcctgat
      121 atgcagatat aaaaatggaa aagtggcgtg gtgaaaacac caggccgtag cagaaggcta
      181 ttctggagag ttaatttttc atttcgggcg tcggataaac agccagataa acgtaaccac
      241 aactgctgag ggtatcggct ttgcaggtca gcccttttgc atacagcgtg acggtatgct
      301 gatggcgggg attcagttca ccgctggtga gcatgagttc cagttgtttc atcagcagcg
      361 gaaaggcctg gtccaggtgg tacgcatctg catcgctgaa caggcctctg ataccggcgc
      421 ggtcggcaag gtaatgcaac cggttaccct cctgcaccag acgtgccccg aaacagggcg
      481 tcacggtgca gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga agtgttgtcc
      541 cggggagtgt gtctgacacg ataaaatcct tacagaaaat cggctaagaa tggtccggta
      601 gtggcgataa ttctgctcat cagaattccc actcagttca gggtgacgct catcagccgg
      661 acatacggac caaaactgtc cttacggcgt tcagcaaaca cggccagcac accgggaata
      721 tcctgcactt cacgaccggt atacgcttca gcactgccgt gccagcggta tttaccggta
      781 cagaacggaa aaagacggga tgtcggatgt tgttggtgaa tacgcatggc ttcaccacgg
      841 gtgataattt tcataatggg atacctctga agacagaaga taaaagtgaa aacaggtgtg
      901 atgtggttgt gaagggttaa agcagaccat gttcggcaaa ggagaaaacc tggttgccac
      961 cgactatcag atggtccggc acccggatat ccaccaggcc cagtgcctgt accagacgtt
     1021 cggtgataag ccggtctgcc ttactgggtg tgacttcacc ggacgggtga ttgtgtgcca
     1081 gtaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg ggatggactt
     1141 ccgtgcggtt gatggtgccg gtgaagaggg tttcaccggc aatcagctga ttctggttgt
     1201 tcagatacag cacccggaat tcttcacgct ccagtcccgc catgttcaga atcagccatt
     1261 cccgtgctgc acgggtggag gtgaaggcca cgccgggttc atgaagatgg cggtccaggg
     1321 ttttcagggc ccgttgaatg aggctgcgct cgccgggcgt catctctccg ggcagaaagg
     1381 aaagttgttg cattgtgctt ctctccattc agtcgatgat gcgcataatg gcgctgcatt
     1441 ccggatgctg cagggcgtaa tcccgcaacc ggtaataatg gaccgtcatg gcataattct
     1501 ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc ggcaataccg gcggcttccg
     1561 ggctcatttc tgcgcggtta ccgttcatgg cattgaacag tacccatgtt tcgtcatcat
     1621 cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt cagggtgtac agattccaga
     1681 taccaccgca gtagtcttcg cacagacggt ccatccagcc gaagacacgg ggctccaggg
     1741 tcacccactg tggaatgagg ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag
     1801 ggacgaggct ggcaaccaac tgaggcggat tattctctgg tgttgtagct gaataaattg
     1861 tgggggtatt ctgagaaacg gttttcatga gataactcct taaataacaa ataaatgtgt
     1921 gtttttcagg aacgaaagct gaatgattcc ctcactcagt gaaacggcat cagcgcaggc
     1981 tctccagcag cgtttctgcc atcacccaca atgcgcgatt gagcttaatg tcggtatcaa
     2041 tgctgtgaat ggcccgggta tggatacgtt ttccttttgc actgcgaccg gaaatcccgc
     2101 ctttcagcat attctcctgg atggtctgat atgcgctcca caggtcctta ccgtaatcct
     2161 cccggcgtcg cggcgtcaga atgtcggcgg tggtgacggg ctgatgttcg tcaccataac
     2221 ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg tggcggcagg accagcgact
     2281 gcatggcatc acgcttctcc tctatccggt caaacacgcc caccacctcg taagcccctt
     2341 cgataacttt ctccactaca tttccccggt gtggaacacg cacttccccc agagactggc
     2401 cacagacgca cccgttctgg cagacgaacc tgaagtaacc cggcagcatc tggtagctgg
     2461 aggtaccgtc atgagagttg agcagaataa tttcagggac atgttctccg tttatctctc
     2521 cggcccgccg cagacgcagc atgtgttttg tgtattcccg gcggctctgg tcgcgcacgc
     2581 gggtctggca ggcaaagaac ggctgaaagc cttcctgctg cagattttcc aggacggtga
     2641 tagtgggaat gtacgcatac cgttcactgc gggaggtgtg ccggtcttcc ccaaaaatac
     2701 tgggtacatg gcgtatcagt tcttcatgtg tcagcggacg gtcacggcgt atctggtttg
     2761 cagcataacc aaaacgactt gctaatcgca tactttgctc cttatcggtg gttaagattt
     2821 actggtgcaa taaatgaaaa aagccgcgtc tcccggagaa gacgcggcct gacgggtgaa
     2881 atgaataagg tttattgtct gagaggcctt tcactggcga gccacgcatt aagcggtttt
     2941 ccggtaacaa tggctggggc tgtatttacc tttgatatta ataatgtgca gagaaacgca
     3001 tggtttactc cttagatttt aagtttgtcc tgggctacaa cgacaggttt tccggatgtg
     3061 tcgatgactc ctaccgtcgg tacgccattt ttgatttcga agaacggttt gttgctacct
     3121 gagcacagtt gattgtcccc gtattcaatc atttgtgctt tcccggtttt caggttaact
     3181 accgagacag tgtagtcgta gcaccaggca ccaccacgtt caagcacgaa aacatagctg
     3241 ttatctttat agttgaactg attctttgtc tggcaattca taccgtcaat ttcggtacag
     3301 gaaaacgctt ctgtagtatt aacgtatttg atgttatttg gcagttttac ttgtttcgca
     3361 gtagcgaaga cagagaacag aatagttaca gcaacgagta aagttttgat aaaagttttc
     3421 atgatagact ccttaaatta gttaaccgca gctacgtagt ttttcaccag atacgcgaat
     3481 tcatgcacgc caatttcacc gaaccaaacc aggtaggccg ggtcatctaa accaaagtaa
     3541 cggtagccaa caacttcatg ggtttttggg ttatagagtg ttacgataaa gtcccagccg
     3601 caggtattgg ctttgcaacc gtcgccctgg aaccagactg taccgttaat gtttactggc
     3661 gtgatacctc caccagggcc atccatatag ccgcctttac cgaacatgcc gctcagtccg
     3721 accggtttta atgctttgtc gatagcggct ttgtaggtag cgtcaccttc gtacaactcg
     3781 tacgttgatg gacacgctcc aggttgttca gcccaggctt ctgggttaca actaagagct
     3841 tgttttacgg tagccgcttc tgcgttaaag ttaaagcccg tgaacagaac cgccagaaat
     3901 aacataaggg tatgtaaacg tttcattgta aatattcttc cgttgtaaat ggcgctggaa
     3961 aatttctcat cagtgaaacc ttccggaagt ttccccccct tgtatgatgt atatcgccag
     4021 tattttattt catcccggct gtaattttta tattccccgg cacgtgcaat aacgctgacc
     4081 agagatataa ggctcattaa taactttaca ggtaatttca taattaaatt tccagtgagt
     4141 gaattgacag gtgaggggta aaactttctc tcaggtaata tatatctgaa aatattttca
     4201 ataacaccag acaggaaaaa caccaccaca ggcggtggcg tttctttggg gcgtctttat
     4261 ttaatccggg ccatcgcgtt tctcagtgcc aaatctgcct cttccgttat atccaggctt
     4321 ttctgcaata ccgcctctga ccgggtcagt gcctgacgtg ctgccagcgg tttccggata
     4381 tgctccatcg ccagcatcgt gtcttcatag ccctgctgac gccaggcgtc ggtatgtgcc
     4441 gatgtaaaac tgagcagacc gccgctgttg ccaccatcac ctgcatattt cagccttttc
     4501 cggggacgga tctcaaggat tgtggtgtcc ggaaaagcct ggcgatccca taaagaaccg
     4561 tcactcagat gcgtcacaat caccatattg catccggcat cgaccagagg cgtcacaggg
     4621 gtatttccct gcatatttcg ccatcctccc ataccaccat caccgaacat tgtcccctga
     4681 acctcacggg gacggaacag caggggcagc gcggctgacg caagcagagc ctctttctgc
     4741 tgtccgcggg gcaggctctg gatatgctga aaaacggcgt ttttcgtggt tccgacaccc
     4801 agttcagcac gaatgcagtc aataatatcc tgcatgcccc cttctgtggg gtacagcgac
     4861 acatacagcg gtagcccgtc tgccagagca tcagtatcaa gataatgatc catcagcgct
     4921 gtcaggggct catctgacaa caacggctga gccatcagat tgtcaaaccc gttgattgtc
     4981 gatatatggc gaagaagcgt cgtaagcagt gctcctgcac gtccggggat ctggcagagg
     5041 ttcattgcct ggaacaattt tttcagcaat gaaaagtaaa ccaatctgtt taccgacagc
     5101 acctgattat tccccagatg atcccagagc gcctccaggc gtacggcagc ttctgacaga
     5161 tcgggagagg ccgcgataat ggcaccattg aatgcgccaa tgctggcccc tgaaaccatg
     5221 ctgatctgtg taccacactc tgccagtgcc ttaaccactc ccgcctgata agcgcccacc
     5281 gccccgccac cggacagcac cagccccgtt ttcatttctg tactcatgca gcccccttct
     5341 gaaatatttc atcgaccata ctttcagaga ggcgttctgc agagcagagg cgaggcatgc
     5401 gaaccggtaa gtgttctgca gcctgcgtgg ttgaccagat caccggatgc tgctcttcac
     5461 tgcaccagtc tcccagccag ttaatcgcgt catgccactc gttatctgcc tgctcccggc
     5521 cctgaccacc aagccagtcc agagtgttca gacgcacggt tgcactaccg ccactctcct
     5581 gtgccatctg cgttaatgct ttatgtctga gaatatccag tagctggtta acctggcctg
     5641 tttcgctctg acgtatcaca tcaccaaacg cgccccagcg aagctcttca agtgccacat
     5701 aacaacgtcc ggcaggggaa aatgacgcat atcgcccggc gctccaccat gagaatatct
     5761 gctcaagatg taactgcgcc cgctgaacct ggtcaacacg gtggagtttt tcttcaagat
     5821 gattcagttt atggtgaacc tgatcggcca gggtgagtaa ctgctcccgc gtatctgcgg
     5881 aataatgcgc cagcctggct gtatcactga ccagtgaact gaccctgtcg tttacccggg
     5941 taatcgcgta gtttgtggtg gcaagtgacg ttgtcatctc cgtcagccag cgcagggacg
     6001 cctcaacgcc ctgtgcaagc gacgcattga tggcattctg ccgtgcagcg ccttcgccgg
     6061 tcagcccctc ttttaaacgc cggaagaacg tgcgctctcc ggccgtgcgg atatgctcct
     6121 gtgcaacatc gattccgttt gcgaaatcaa ccacaaattt ttcaggaaga caggtgagta
     6181 cctgacatac ccggttatca gcaatttgtt gaataaatgg tgaagtcaca gcattgtctc
     6241 cagctcttcc ctcagcgcct tactgtcagt aatgagctca ggaacccttt catttaatgc
     6301 ctgaagtcgc ccggtaagcc gttcctgcgc ctgctgatcg cgtttatgat cttcagagct
     6361 ctgaatcagc gtgttacgca gttgttcaac tttctccctg aatgcgcaga aaaaggcatc
     6421 gatttcctgg cggacgggct ggttaatatc ctgctcaatg gatgcctgca gctccccgaa
     6481 atatgcccgg gtcagtgaca taacttcctc tctgaccttg ttgatattga tcacgctgcg
     6541 actcacatcc tcgtcatagt tttcccagcc caggtcactg gtgccaaacc atcgacaaac
     6601 ggttccccat acgccactct gccgacggcg acgggtgacg gtctccgttc tttcctccat
     6661 aagcgcactc agatttattt ttaccgcgag ccgggtctgt aactggctga ctgccggaaa
     6721 actgattttt acggtaaagc cctcatcctc caggcgggca ttgatgcgac cggcaatgtt
     6781 ttctgtggcg tacagggccg tgccacgaaa acctgtttca atcccgctga cgattttctc
     6841 cagctcaggc cggaactgtg cttcagcctc acggtgcagg ctggtcacgg tcgattcgat
     6901 ttgcgtcatc agttcaaggg cttccctgcg atcactgaat tttatttcgg ggctgtccgg
     6961 ggcaaaatct cgtttcctgt aatcctcccg gccctggttg ttgataccgg accattgaga
     7021 aattttcccc cataatccgg actgagacct tcgttgttct gcgtcttctt cctccagcat
     7081 ttgctgttct ttgcgtttac cgcttctgaa atagtcattc agctcctctt ccagtgaggt
     7141 gcagaccccc gtcagtaatt cccgggtttt agtatcgatc tcctccatgg ccattctgac
     7201 atcagtggtc atctgctcct gacagtccgc gatttcccgg atatccgcaa gcaggctggt
     7261 gatatgcgac tgcagtgact gaatgctttg ctgtagcccc tgatggcgta acaacaggta
     7321 ttcactgata ttttctgcat tctgcatcag ttttgcggcg gcagagtcaa ccgccagtgc
     7381 cgcagctctg gaatgtgagc tctgtatgac ttccgttatc agttgatcga taagtgagcc
     7441 ttcccacagg tctgtcgccc cttcagttgc cagttcactg tctttacaga cgtattcttt
     7501 tttcatgcaa ccgaaggcac ctctgataaa atcatcgacc caggcttcat caacaggaag
     7561 ggttccgttt ttccgtaact catgaagcgc acggttagcc aggtatgcct ggcgtgagga
     7621 tccgggataa acgcgggagg cgggcagcac atcgctgttc agcattgccg gaactttctg
     7681 gcgtacggca tctgccccat cgccattgcg gtctttctca tcaaatttat tgaccagtac
     7741 aaacaggcgg ccggctgata cgtcagcaat ggcattaagc tctttacgga cgtcttcatc
     7801 cgcttttgag ttcatctggg tgtaatccat cacagccaga accgcagagg ctttctgcag
     7861 ttgatcccgc atcatcactt ccatctgcgg ttgtccggcc tcattagggc cgggggtatc
     7921 cagcagtgtg agtgttccct ggcatgcatc catccccacc agatggctga attccacgtc
     7981 gatcaccggc agtttctgca cttctgcgta ttcatcaaaa ggaaattcag tccccattgc
     8041 cgcagcaaga cgaaccagat cgtttaacga tgccagtccg gtaaagattt cctcctcccc
     8101 gtggtattca tttttgagcc agccatcatc cgtcagaata tccagcagtt cgcgggtatc
     8161 cccggtttgc tgcagggtct gtgcgacctg ctgtcctgcc ggggtggcga gtttttcctg
     8221 cagtgtgatt aataaattgc ggacaggctg aatatgttcc agatggagaa ccggctcagt
     8281 ttttccggga acgtggcgga tgagcgtcgg tacagaggtc atggggcggt tacggttagg
     8341 cagaatttcc tgccccacaa tggcgttgat ggtggttgat ttgcctgctt tcatcgtgcc
     8401 gacaacagcc agcaccatct ccctgcgggc ggttttgatt tgttcgccct ccagctcctc
     8461 aattcttttc agtgctttct gtttatcaaa aagcagtctg ttttcgttct tactgtcaga
     8521 taacacatct ggctcctcaa ccatttgccg gagcagatta atgttcagtt gcaaaagtcg
     8581 gtcggcttca tcacaaagca gggcgatgtt cttttcgtgc attatttaac cttcctaaga
     8641 cgcattttca tgacaaccac ggaggaggtc atttactaca aacgatcgtt tataacgatc
     8701 gatatgatag taacgatcga tgtattctat taaattgaga aacacaacgc aaggggacaa
     8761 cccgggtatg gctgcgtttg catagtgttt tcatggagtt aaaaatctgt aacaatagcc
     8821 tggtacatca ccaccgggtt cagaagaaaa tccagttcca taccgcgcgg gcaacagaga
     8881 ccaccgagtc acggacggcg cgcagaaccg tgcgtgcaac agaggcaatg cagacgctct
     8941 ccgccgtgtc aaatatccgg tccaccgcac cggtaaactg ttcacgggcc tgagaccgga
     9001 cagactccgt gcgcagctcg tcctgcaggc gggtcatcag gggactggcg gcatggtcgg
     9061 gaagtgctgt catgagcgca ctgaccagcg tatccagttc ccagccggtg cgggccgata
     9121 cggcaacaac ccgatgtacg ggccggaaca gacggaatac cgcctccgtt ttttcgcgaa
     9181 tgttctgtgc ctgtgcggga gaaggctgaa taccggccat atcccattca tggcagggct
     9241 ccgttttatc ggcctgcatc accacaaaca gtacccgctg atgtccccgg tgcaggatgt
     9301 gtcgccagaa atactcatcc acagacaggg cacggtcatc ggctttaatc agccacagta
     9361 ccaggtccag ttcgagcaga atgtcacggt acagggcttc atactctgca tccctgtccc
     9421 ggctctcgcc caccccgggc aggtcagtga taaccatgct gtgaccatgg ccattcagac
     9481 ggaagcgctg cacttcccgg gggccggcgt gaacatcact gaccggggtg acctccccct
     9541 gaaacagcgc attacagagt gaggatttac cggccccgct tttacccata atgccaggca
     9601 cgggttcgtg actggtgagt ttgcgcagat gttccaggat gtgacgggaa agtgagtaag
     9661 gcagggagga gagcggtttt tcaattgcct caatggcatc agacggattc atacatgtat
     9721 tcccggtgaa aaaagacaaa accccgcgca ccggaaaggt ggcgggggat tcacggaaat
     9781 aatatctgtc tgaaattatc tggaatcgat ttggctgttc tgcctggttg ccaccaaatc
     9841 atggtacgcc tgctcaatca tttcattcag cagttttttt gggaggacac cacgacttcg
     9901 ggcaatactt tcaagctgtt tgtggatttt tccggacagt ttcagaggga cggaaccatc
     9961 agcacgttca tcacgccttt tttgttgctc ccgggcactt ttcagctcat caagaagaat
    10021 cgctttatgc tgaatcctgg gggcctggaa ggagacctgc gtaaacttcg ctctgttccg
    10081 tctgggatta ctttccagta aaaactgtac ctgttgctca ttccagccct cccagttatc
    10141 aaacgtggcg caggcaaacc agtatttctg ctccggggta aggggattca gcggggttcc
    10201 cacacaccgg acctgcatgg cgttccataa ccagtcacac tgttcactgt ccctgctgtc
    10261 cacccacgaa aagggggagg ggtaatcatt gtttatgctg gcctattcag agaatactga
    10321 gttgataatc gtcctcgtag cctctggtcc ggatttcagc aggtaactgg agcagaacca
    10381 tataatttca cggtagattt tatccggatg cacggtgcgc atcggctttt ctctgggacc
    10441 ggcaatcttc ggttgcgatg gaaaccccgt ggcaaaccgc agatgttccg ggggcattcc
    10501 tgaacgtttt tcaggaatgg gtgtgagtcc tgcgtttctg gtcttttccg cttgtccggt
    10561 attgcctccg gccggagtgt tgtcgggagt atcttccgga ggcgaggtgt tttcattagt
    10621 tgggggaaca acagcagaac tgtccatctc cggaatgggt tgaggggcat aatcactgtg
    10681 gtagccaggg gaaagcgtta agtcccgaag ggtgacatca ggttccgggc agtgagtgcc
    10741 accgggagtg aacgggagtt gttgcttttg ggagaaaaat gtattgtgaa tgcgggtata
    10801 cagctggaac agaaaaaaat aagatgcccg tggttctttt tttaaccact gtgtatattc
    10861 ctctatggca ccgagctgtt gtttgtgccg ttttacagca tcgatatacg gacagctggt
    10921 gcagatatca tccagttcct ttcccacaat acacctcacc tgcttttctc acatttgcag
    10981 attttaaaaa agcaaacaaa aacctccgta catgatgtac agaacaatct accaggtagt
    11041 ggcgtcagaa aacctccggg ccgcctgcac tttccggaat gcgccggtta tgaaaaaccg
    11101 gaaattcagt ctctgttgta agatgtaccc tctgatacta cgttttctct gtgtatttaa
    11161 ctcactgtat ttattgtctt tttaagcttg actgatattc tccggtcata gtgtgccgga
    11221 tatacatccc gtaaacttct catttttata taaaaatacc tgcactctct tcagcgcatc
    11281 tgtctccttt aagtaaaaaa tgttttcacg tattgctgta aattcatttt aaatcagtat
    11341 attaccttga tattgctgtc tcctggtttg atgcgtaatc gttctgcctt tctggatgat
    11401 tctggtgctt ccgggtgcat tctcctctgc acatgcaaca gttcgtcatc gccacgctcc
    11461 caaaaagcat ataaaaacga atcgaatttg atatttttct caatcgtttt tcataggaat
    11521 ttcgtagggt attctccgct ataacctcca tatttgctgt ctttttcttc ataaatcgta
    11581 tgcaatatag cctgtctgtc aagacctctt ctctgctgtt gaggagacga tctcattttt
    11641 ggtcttttcc gggagagagg ctgtcagatg agcgagccgg atatactgtc agaggtacac
    11701 cccgtagcag acctgtgctc ccatttccgc gatccggaac caacaacacc ctacggttaa
    11761 taccatttga agcgtcaggt actgcaggaa gttcccccct gcgcggacat cggttcctgc
    11821 cgacatctca caatacggcc agactgtcat gcagtaacac ccgcatgcgg tctgcggttt
    11881 taagcgcact ctcccgtttt cagtgcaccg ataatgcgtc atagtaagtc tgggggaagt
    11941 tacggaatgc ctgttaagtg gtgccgtggg tggagacagc ttacttttct acagcaactg
    12001 cagtcattca ggatgatcag gagatattct gtcaatgtca cgatcctgcc attttcgtac
    12061 ccgctaagat acttccgtta ctgatgctgt ccgcttttca ccatttcatc ctcactgaca
    12121 ggcgttgcgt tttgggtcat caccacagct ttacgtttct cttttaaccg gtaacgctct
    12181 cctttgatat tcagcgtggt tgagtgatgc agcagtcaat ccaggagcgc tgttgtccgg
    12241 caaccaccat atcaacttct tcccgggtgt ggcggagagg tggtatcctg gctgagtttc
    12301 gaagcgaacc gttctttttg acgggcgcat tttacgtccg ggctgaatgt agtaacgcgg
    12361 catggaacgt cggcccgtat accattgcct taatctccgc aaagataacc tcgtcattcc
    12421 ggacattctc tgccaggcat atgtcgatgt aatccataaa cgatttcagc ttaccccttt
    12481 tgttgtcacg aacggtgcaa tagtgatcca cacccaacgc ctgaaatcag atccaggggg
    12541 taatctgctc tcctgattca ggagagctta tggtcacttt tgagacagtt atggaaatta
    12601 aaatcctgca caagcaggga atgagtagcc gggcgattgc cagagaactg gggatctccc
    12661 gcaataccgt taaacgttat ttgcaggcaa aatctgagcc gccaaaatat acgccgcgac
    12721 ctgctgttgc ttcactcctg gatgaatacc gggattatat tcgtcaacgc atcgccgatg
    12781 ctcatcctta caaaatcccg gcaacggtaa tcgctcgcga gatcagagac cagggatatc
    12841 gtggcggaat gaccattctc agggcattca ttcgttctct ctcggttcct caggagcagg
    12901 agcctgccgt tcggttcgaa actgaacccg gacgacagat gcaggttgac tggggcacta
    12961 tgcgtaatgg tcgctcaccg cttcacgtgt tcgttgctgt tctcggatac agccgaatgc
    13021 tgtacatcga attcactgac aatatgcgtt atgacacgct ggagacctgc catcgtaatg
    13081 cgttccgctt ctttggtggt gtgccgcgcg aagtgttgta tgacaatatg aaaactgtgg
    13141 ttctgcaacg tgacgcatat cagaccggtc agcaccggtt ccatccttcg ctgtggcagt
    13201 tcggcaagga gatgggcttc tctccccgac tgtgtcgccc cttcagggca cagactaaag
    13261 gtaaggtgga acggatggtg cagtacaccc gtaacagttt ttacatccca ctaatgactc
    13321 gcctgcgccc gatggggatc actgtcgatg ttgaaacagc caaccgccac ggtctgcgct
    13381 ggctgcacga tgtcgctaac caacgaaagc atgaaacaat ccaggcacgt ccctgcgatc
    13441 gctggctcga agagcagcag tccatgctgg cactgcctcc ggagaaaaaa gagtatgacg
    13501 tgcatcttga tgaaaatctg gtgaacttcg acaaacaccc cctgcatcat ccactctcca
    13561 tctacgactc attctgcaga ggagtggcgt gatgatggaa ctgcaacatc aacgactgat
    13621 ggtgctcgcc gggcagttgc aactggaaag ccttataagc gcagcgcctg cgctgtcaca
    13681 acaggcagta gaccaggaat ggagttatat ggacttcctg gagcatctgc ttcatgaaga
    13741 aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag ccttcccggc
    13801 ggtgaaaacg ttcgaagagt atgacttcac attcgccacc ggagcaccgc agaagcaact
    13861 ccagtcgtta cgctcactca gcttcataga acgtaatgaa aatatcgtat tactggggcc
    13921 atcaggtgtg gggaaaaccc atctggcaat agcgatgggc tatgaagcag tccgtgcagg
    13981 tatcaaagtt cgcttcacaa cagcagcaga tctgttactt cagttatcta cggcacaacg
    14041 tcagggccgt tataaaacga cgcttcagcg tggagtaatg gccccccgcc tgctcatcat
    14101 tgatgaaata ggctatctgc cgttcagtca ggaagaagca aagctgttct tccaggtcat
    14161 cgctaaacgt tacgaaaaga gcgcaatgat cctgacatcc aatctgccgt tcgggcagtg
    14221 ggatcaaacg ttcgccggtg atgcagcact gacctcagcg atgctggacc gtatcttaca
    14281 ccactcacat gtcgttcaaa tcaaaggaga aagctatcga ctcagacaga aacgaaaggc
    14341 cggggttata gcagaagcta atcctgagta aaacggtgga tcaatattgg gccgttggtg
    14401 gagatataag tggatcactt ttcatccgtc gttgacattt gtggccagtc tttctggctg
    14461 gcggttcagg ggatgttttc tggttatcgt gtaccacaaa gcagggatat tgccccgata
    14521 gtcacatttc agattgatcg cggtctgaag atgtggcact gaggtaacgt gctggtgtca
    14581 ctggtattgc tgaagttatg gtttttcggg gtcaggtgag cctgttgtgt gtatgctttt
    14641 tgttcaggtc agagggggac tgtgtggaca gcggcttttg tctccgggca ttcacgcctg
    14701 aacgatgaat gcgattttca gcaatatttg tctttgtgac actctctggt tgagaagcta
    14761 atgcgcgccc ctgtttccac agagaatgag gcttgccatc agccgaaaca tcagtattcc
    14821 acgccataca gacggcgcct gaccatgtgc tgatgcaggt aaaatgtttc actacaactg
    14881 ttcgggttgc tatgcaacga aacagcgatt ctcacgatct ttaactgcaa cgtttttccg
    14941 cctgaaagct gactgcctcc tgctgaggtg ggaggctatg attcagtgcg tttatagtct
    15001 ccggggtatg catgtaccgg agcataccat ttctggtgga taacaagccg gcacattcag
    15061 actgcagtga agatggaggc acctggttgt gaagaacgtt tctgcccgtg gctgcttcgc
    15121 atcatcaatg gtattcatac ccgctcagaa atgaccgggt gctgaagccc tccgttatcg
    15181 cgcagtataa tgacgtatat gatctgtgga cgagtgagcg gcgtgcctga tgtacgccgc
    15241 tattactgta ttttaccaca gggctggttt tctgctgcca gccctgtggt tctcagacat
    15301 caactgtgag cggcatcaac cgatgtacgg tatgccataa gcacgtactg atccatcaga
    15361 tttgtcagtg gttcactctg accctgacgt gcaatgatcc attgctccag atctttttct
    15421 gatttgtgtt tcaggttttt cgtccagtcg tcaaggctgt ttccggtcat taagtcaagc
    15481 cagcagagcc agaccagttt gagacgtaac tccgtgccgc caaactgacg gaagaggctg
    15541 atcagtttgt ttgccagaga atgttgcttc aggcgctcac attcagacag cagaatccct
    15601 tccagcatcg gatgatgaac cagcaggtgt tcgctggtgc ataaattcac caccagatca
    15661 tcatccaggg acggggagct ccgtttgtcc ggaccataaa tcagccggtg cagactctgg
    15721 ggaacgatgg caatctccgg tttttcgcca ttgaatgaca cccacccggg cgtcagatta
    15781 tggaaatagt agatattcgg atgacacacg ccccatgata cggcgtgccg ggtgaagccg
    15841 gtcagccatt tctggtagtt ttcatggagc agctcgtaac gggtctgata ctgttgtttc
    15901 attttcatat tcctcacggg ggataacagg cagcagagcg ccgccggcag gccggagatt
    15961 gcgtctgctg tgggcgaagg gttagtggtt aaaaattacg gatgggactg tgcaatacgc
    16021 gcctgcagcc aggcttccac ttcacttttc agccagcggg agctgcgccc cattttgatg
    16081 ggcgcaggaa agcccccatc tttgatgagt ttgtcaaacc acttatcggt taggccggtc
    16141 agttgagtga taaacgccat gtcgaccatc tggtcatcca tcagcgaaac tggggttgcc
    16201 atgagaatgt tcctccggat gttgttaaac cagccggtgt ggtcatgaaa acagcgcgcc
    16261 accttcaccg gcataacaac cggggagaaa cgtaaaaaat aatcgggaat gtggctgtcc
    16321 ggagataaac ggggggattg cgatggcaga gccgtagcgg aatcccgggg gggttgttgc
    16381 gaaaagatgc ttccgcacgc acgcgcgata aagaggggat agttttcgtg aggacaggga
    16441 gagaagcagg cgttctgata attgcagagt tgttttcgga ggtggttaca gtgagtcagc
    16501 ccgttcctgg cgagcaggag actgatatca ccgatgggat aaggttgtct gattggtatt
    16561 ataaatataa gaagtcatca gagagtaacg taaaggattt gccagaataa cctaagagag
    16621 taaatctctc cggtaacaga gcgctgtgaa gcattctgta tcagagtgca gatacatgaa
    16681 cgctgatgcg taaaatcagc agtgacacgc cagctccccc tgtgcccatt cctgtataag
    16741 acatgaactg agcgtattaa tgctcctgcc ctgagactga gccggtgagt gaggggctga
    16801 gaaatccctc ttgacactgc tctaaaccag aaatacttcc ccggttgttg cggtgcgtcc
    16861 ggatccccga taacggaaca ctaaggctgt attgcgggac accaggtaag tggcagcccg
    16921 taatacgtca ggagacgcgg gaaaagtgaa ttcccacacg cgttataaac ttacccttgc
    16981 ggagctggaa gcctttaact ctgccgttga caaccggctg gcagaactga caattaacaa
    17041 actttacgat cgcgcgccgg cttccgtctg gaaatatgtc acctgaatat tgaagcagcg
    17101 ctacgatgac tccggtgtta tctgcaccgg aggaccggga acattaagcg gtatagcgac
    17161 aacaaagaat tcgaaatcaa aagggaatgg tgccggaggc gtgggcggta ataggctgat
    17221 tttcaggtat taccggcctg tatctgctga agggggctcg tatttgtcgg ggctgcgtat
    17281 tccgctcgat tcgatcacgg attggcggaa tacctaactg tgattgattc aggtaccatt
    17341 ttgcagggat ccggtgcgtt actgtctggc acaacaaacg cagcgggtcg ctctgctccg
    17401 ccgattcctg ataatccctc cccagtaacc tactgcaaca gtacaaatac actgacgcct
    17461 gaaacggtaa ccagaatcag caccagcagt aactcagtaa acagagcaga cgatcggtct
    17521 tccggtaata accggaagga acgacagcgc cacaatacag gcaacacact gtattatgag
    17581 taatcgaatt accgcaatcc ccgcatttct ttttgcgtat cacgatagct ccctcgccac
    17641 gccttatccg taaccggttt tttacattaa aaaaaacaac gccgggaatt caatcgtcag
    17701 tccggagacg accgttcggg ttatcacaga gagcctgagc aggggggctg cgcatttttt
    17761 acgatgtggc tattccgtat gaaccatacg gagatataac catgacctac aaatacaacc
    17821 ccttctggca gcaacgtatt cgtgagacgg tgcggcacgc actgaatgtt catccccgcc
    17881 tgacggcatt gcgggttgac ctgcgtttcc cggatgtacc ggcagcaacg gacgcagctg
    17941 tgatatcccg cttcatcaat gccctgaaag cccgaatcga cgcttaccag aaacgtaagc
    18001 atcgggaagg taaacgcgtg catcccacaa ccctgcatta cgtctgggcc cgggagtttg
    18061 gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg gatacctggt
    18121 gtcgtgccgg tgattaccgc gctccggagt ctctggccgg gatgattaaa caggcgtggt
    18181 gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt ccggcctggc
    18241 cggcggtgtg gctggcgcgt aatgatgaca ccggatttca gcaagtgctg gaacgtgctg
    18301 actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac tttggctgca
    18361 gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt gttcacggcc
    18421 accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc ggaccacgct
    18481 gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca tatactatgt
    18541 acgcaaaatc ctttctcgct cttgatagca acggacgtct gacgggcgcc cgtacagcac
    18601 aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc agataccatc
    18661 cgcaatacga cactgaactt ccttggtttg aacacactga cgacaggctg acagagcacg
    18721 gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt aaacgtctgc
    18781 agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac tgcagacaat
    18841 gtcaccacga ttattatggg gagcggtact gcacacactg ccagaccgga ggtttcagtc
    18901 ttccccggac agctcagcaa ctgtcttgaa cgtaaaaatt atggagcaac gtgatggtac
    18961 gattcattcc attcttcgtt ctttctgagc attgcgttca ggatagtcag cagcttgcgt
    19021 atacaggtaa ccagcgccac ttttttgggt tttcctgcgg caagcagtcg catgtaaaac
    19081 acctttatta ccgggttaaa gcgggttgcg acaagcgctg ccatgtacag ggctgttcgc
    19141 acaccagccc tgccgccaaa gatggttcgt cgccctctca tcgtgcccga gtcccggttt
    19201 actggaacca taccgacaag cgcactgatt tcacgcctgg agaggttacc ctgttcgggt
    19261 acttccgcca gcagcgcagc agccgtcatg gtgccaacac ctttaatact gctcagtcgc
    19321 ttagcaagct ctttgaagtg gctatggata tggctgttca tgtcagcatc tagtcgcgca
    19381 agctcgtctt ccagtgcttt gatgatgata ttaatgcttt tcctgttcta tggatgagca
    19441 gggtgcaacc ggtttctctc ggcaaccagc atcgtaatca actgccgcct acgaactacc
    19501 attgcagtga gaacctgaca ttcagtatcc ggcagtgctc tgataaagcg ttcccgctcc
    19561 ggatgccggt tgagaacctc agccaactgg gaaagcactc tggcatcaat actatcagtt
    19621 ttcgcgaggt atcccatggc gcgggcaaag tcacgagctt gtctggggtt aaccacggca
    19681 agctcaaaac cttctgcctg aagcgcacag actatggcgg tttcaagtcc tccagtggct
    19741 ttcattaaaa ccagcgcaat ggcgtacatt ctcaactcag caatgatggc gtcaaagcca
    19801 tcactgtcat tgccgacaga aaattgttca atagcgctgc tggcggcaat atccagtatc
    19861 gctttcgaaa catcgatatc cacacaaagt gaacctggct gactcatgat tacccatcct
    19921 tgcaaatgcg ttatggcgta ctgacaactg ttcgggtttc agatgagtgg ctcagtgtat
    19981 gcgccattcg ctgagctacg ggctatgaac cccttgaggg atcgggctgc atacaccttg
    20041 ccaaatctaa tcacagttat tttaaccaat tgtcaagata caaggaggag atatgcgaat
    20101 tctgaactgc tatatggcga atgacagcaa aggccatttt gttacggcga aagaagccgc
    20161 gaagcacaac cgacaggacg ttttgtgctg tgtgtcctgt ggttgtccgt taacacttaa
    20221 gcggggaaat gacggacaac cgccgtggtt tgaacatgac cagatgactg ttgctgcgaa
    20281 aatcctgctg cgatgcacct ggcttgaccc ggcagagaaa gaggcccgtc gtttgcatct
    20341 gcagggcatg acggttccgg attatacggt gaaggtgaga aagtggtttt gtgtgatgtg
    20401 tgacgaagat tatgaggggg aaaagtgctg cccgcgttgt ggtaccgggg tatacagcag
    20461 ggagaggggg gctgcaggag ggcaactgga aggataggaa ctgagcggtt cagtcgcgga
    20521 aatcagttgc agagctgtaa tgatatctcc tggagtgctg cgttggtagg ggcatcccgg
    20581 gatagagaaa ttttagcact ttgccctgaa tgattgtaga cgtcccgagt actcgtaccg
    20641 gcctgagtta ttacagttag atattcgctt tttctcccgc atacttcaca gctgtcaacg
    20701 acggatgaaa agtggttctt ccgcaacttg ggtggaagaa aatggatgaa actttctggt
    20761 gaacaaggat ctaagctgat ttaagttatg ggcaacgcaa tgcactctct taagacactt
    20821 ctaaagttac cttgcggatg gcgatgcagt cgacaaatta ttagctctga cggtataacc
    20881 ctccatctcc acggaaaacg caaaacagca caatgtcctg aatgctttaa gcgtagcgac
    20941 tctgttcata gttgtcgtcg gcgccggata cagcatctac cctgctcagg gcagacgcta
    21001 tggcttgtat tttccgtccg ccactggtac tgccgtaacc cggcttgtca cgtaaaattt
    21061 ttgccgagtc gcttgctccc ttcgccagtt cacaccagca gtcttcacag gcgttacaaa
    21121 attaacaacg tcaactggga ttaatagctg gaggtgaggc tggaaaacgg gctgcaacgg
    21181 cagcgggtct ccgttgcagt gctgatactc ttcttcgcag gcttatcaat accccggaga
    21241 cgaaacagtc aggcgcgcct catgtcggta ttgatgagtg ggcgtggcat tggggccacc
    21301 gttacggtac gttaatattc aatcttgata ctcaccgccc cctggtcctg cttcccggcc
    21361 gtgatcagcg tacgctggcg acctggttca gaaaaggtcc ggaaatacag gttgtatcgc
    21421 gtgatcgcag tggagtctat gcgacagcag cacgtgaagg tgcacctcag gccagacagg
    21481 tggcctatcg atggcacctg ctaaaaaata ttggtgatga gcctgaacga atgatgtaca
    21541 gacatatgcc tctgatacgt cttgttgtca gagagttatc actgaagaaa tcacctgagc
    21601 cagaaatatc tgtgcctgta gcatcgctcc gtcgtctgga acgccttaaa cagcacaccc
    21661 gcaaaaaacg gcatcagcgt tggacagagg ttatggccct gcataacaag ggatgtagtt
    21721 tcagggaaat atcccgtatt acaggcctgt cgcgagtgac agtcagtcgc tgggtgggtt
    21781 caggaacatt ccctgaaatg tcaactaggc ctccaaagcg agggcttctg gacccatgga
    21841 gggagtggtt aaaagagcaa cgagaatgtg gtaattataa ctccggccgg atatggcggg
    21901 aaatggtggc caggggggtt acaggcagtg aaaccatcgt cagggatgct gttgccaaat
    21961 ggcgtaaagg ctggatccca ccggttacta ctgccgcaag acttccttca gtgtcccggg
    22021 taagccgctg gttgatgccc cggagaataa tcaggggtga agaaaattat gctttccgat
    22081 ttattagtct gatgtgtgaa aaagaaccgg agttgaaaat agcgcagcaa ctggtactcg
    22141 agttctaccg tattctgaaa acctaaaata aatcacagct aagcagctgg ttcactcggg
    22201 tccacgaaag cggctcagca gaacttcggt gcgtagctgc ggggatggaa gctgatgctg
    22261 cggctatacg tgaggcaatc agcagtcgct ggagtactgg tgttgtcgaa ggtcatgtaa
    22321 atcgcctgaa ggtgctgata cgccagatgt atggtcgagc cggacttgaa ctacttaggc
    22381 ggagggtaat gagtccactg gcatgaaaat cttcacctag gttgcggaag agccactttt
    22441 gcactgttcg tgacagtatg gcctttatgt actcactttg ttaccggtag ctggatcatc
    22501 actaccgtgc tgcaaatggt ggtgaacagc atcaattgtg gaatgaatgt tgccgaagcg
    22561 accaatgcgc ctcgtttcca ccatcagtgg ttgccggacg agctgcgtgt tgaaaagggg
    22621 ttagtccgga tacgctcaag ctgctggaag caaaaggtca gaaagtggcg ctgaatgagg
    22681 tgttgggcag cacgcaaagc attatggttg ggtcggatgg tgagttgtac ggcgcatccg
    22741 actcgcgctt ggtggatgat ttaacggcgg ggtattaatt gacatccccc ggcgcaatgt
    22801 gccgggggat aatggtttta tgcggtcaat aatcgtccgg gattatcgat acagagtttt
    22861 ttgaggatcg ttttatcgac gccgcgctcc gccagatagg ccagaaaaac atctggaaca
    22921 aatccccagc cattaccgcc atttttagcc cacatttgtt taaggaagac atcgtggctg
    22981 agaacaagct gatcggcgta tcccaactca atgagatgag cgacggcatc ggcagtctcc
    23041 tgcacccctg gcgctattcc ctctttcggg aaggtaatgt ctaggccaat catgtcgaat
    23101 tccagccaga cacctctatc aagcattttc cgctgatacg ccacgtcttt tcctgacgga
    23161 tccgagtgtg cgagagagac tttatttggc gagacgccca tttcacctaa cacaatgtcg
    23221 agtacttcat caccgcgacg aagccagccc ggcatgtgaa tattcatcgc cacatgagga
    23281 ttgttaatct gtgccagcga ggcagcccgc aagctgttat gctcggcttc ggtaaatgtc
    23341 ggttaaacac ttatttcacc gatcattccg gcacgaatat ccgtatcgcc aatcccttga
    23401 ttcaattctt tatcgatcg
//
LOCUS       DAZFZC010000082        22649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFZC010000082 DAZFZC010000000
VERSION     DAZFZC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.082"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..252
                     /locus_tag="ACHKXP_003598"
     CDS             <1..252
                     /locus_tag="ACHKXP_003598"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7648530.1"
                     /translation="NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFD
                     ARTAIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADS"
     gene            325..576
                     /locus_tag="ACHKXP_003599"
     CDS             325..576
                     /locus_tag="ACHKXP_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7648531.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            611..1891
                     /locus_tag="ACHKXP_003600"
     CDS             611..1891
                     /locus_tag="ACHKXP_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO7648532.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKDVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(2079..3098)
                     /gene="rspB"
                     /locus_tag="ACHKXP_003601"
     CDS             complement(2079..3098)
                     /gene="rspB"
                     /locus_tag="ACHKXP_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO7648533.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(3110..4324)
                     /gene="rspA"
                     /locus_tag="ACHKXP_003602"
     CDS             complement(3110..4324)
                     /gene="rspA"
                     /locus_tag="ACHKXP_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO7648534.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4530..4856)
                     /gene="ynfA"
                     /locus_tag="ACHKXP_003603"
     CDS             complement(4530..4856)
                     /gene="ynfA"
                     /locus_tag="ACHKXP_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO7648535.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4991..5332
                     /gene="ynfB"
                     /locus_tag="ACHKXP_003604"
     CDS             4991..5332
                     /gene="ynfB"
                     /locus_tag="ACHKXP_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO7648536.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5367..5927
                     /gene="speG"
                     /locus_tag="ACHKXP_003605"
     CDS             5367..5927
                     /gene="speG"
                     /locus_tag="ACHKXP_003605"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO7648537.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5930..6640)
                     /gene="ynfC"
                     /locus_tag="ACHKXP_003606"
     CDS             complement(5930..6640)
                     /gene="ynfC"
                     /locus_tag="ACHKXP_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO7648538.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSQEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6748..7053
                     /gene="ynfD"
                     /locus_tag="ACHKXP_003607"
     CDS             6748..7053
                     /gene="ynfD"
                     /locus_tag="ACHKXP_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO7648539.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            7252..9678
                     /gene="ynfE"
                     /locus_tag="ACHKXP_003608"
     CDS             7252..9678
                     /gene="ynfE"
                     /locus_tag="ACHKXP_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO7648540.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKCKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            9739..12163
                     /gene="ynfF"
                     /locus_tag="ACHKXP_003609"
                     /pseudo
     CDS             9739..12163
                     /gene="ynfF"
                     /locus_tag="ACHKXP_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
     gene            12174..12791
                     /gene="ynfG"
                     /locus_tag="ACHKXP_003610"
     CDS             12174..12791
                     /gene="ynfG"
                     /locus_tag="ACHKXP_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO7648541.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12793..13647
                     /gene="ynfH"
                     /locus_tag="ACHKXP_003611"
     CDS             12793..13647
                     /gene="ynfH"
                     /locus_tag="ACHKXP_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO7648542.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13690..14304
                     /gene="dmsD"
                     /locus_tag="ACHKXP_003612"
     CDS             13690..14304
                     /gene="dmsD"
                     /locus_tag="ACHKXP_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO7648543.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14463..15755
                     /gene="clcB"
                     /locus_tag="ACHKXP_003613"
     CDS             14463..15755
                     /gene="clcB"
                     /locus_tag="ACHKXP_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO7648544.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15708..16403)
                     /gene="bioD"
                     /locus_tag="ACHKXP_003614"
     CDS             complement(15708..16403)
                     /gene="bioD"
                     /locus_tag="ACHKXP_003614"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7648545.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16528..17748)
                     /gene="mlc"
                     /locus_tag="ACHKXP_003615"
                     /gene_synonym="dgsA"
     CDS             complement(16528..17748)
                     /gene="mlc"
                     /locus_tag="ACHKXP_003615"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO7648546.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17883..18776)
                     /gene="ynfL"
                     /locus_tag="ACHKXP_003616"
     CDS             complement(17883..18776)
                     /gene="ynfL"
                     /locus_tag="ACHKXP_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7648547.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPESLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18883..20136
                     /gene="ynfM"
                     /locus_tag="ACHKXP_003617"
     CDS             18883..20136
                     /gene="ynfM"
                     /locus_tag="ACHKXP_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648548.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(20185..20304)
                     /locus_tag="ACHKXP_003618"
     CDS             complement(20185..20304)
                     /locus_tag="ACHKXP_003618"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO7648549.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20560..20868
                     /gene="asr"
                     /locus_tag="ACHKXP_003619"
     CDS             20560..20868
                     /gene="asr"
                     /locus_tag="ACHKXP_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO7648550.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETTTTPAPTATTTKAAPAKTIH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAKKHSHQQPAKSAAQPAA
                     "
     gene            20961..21044
                     /locus_tag="ACHKXP_003620"
     CDS             20961..21044
                     /locus_tag="ACHKXP_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648551.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            21144..21965
                     /gene="ydgD"
                     /locus_tag="ACHKXP_003621"
     CDS             21144..21965
                     /gene="ydgD"
                     /locus_tag="ACHKXP_003621"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO7648552.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVARSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(22004..22333)
                     /gene="mdtI"
                     /locus_tag="ACHKXP_003622"
     CDS             complement(22004..22333)
                     /gene="mdtI"
                     /locus_tag="ACHKXP_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO7648553.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(22320..>22649)
                     /gene="mdtJ"
                     /locus_tag="ACHKXP_003623"
     CDS             complement(22320..>22649)
                     /gene="mdtJ"
                     /locus_tag="ACHKXP_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO7648554.1"
                     /translation="ATEITGTLSMKWASVSEGNGGFILMLVMISLSYIFLSFAVKKIA
                     LGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLIKSGTRKARKPEL
                     EVNHGAV"
BASE COUNT         5856 a         5683 c         5473 g         5637 t
ORIGIN      
        1 aatggagcca acttcgacaa cacgattttg cgccgttcat acgaacggca ggggatcccc
       61 tgcccgtggc gttactacaa cgatcgcgat gtacgcacaa tcgttgagct ggggaaagcc
      121 atagacttcg atgccagaac ggctattcca ttcgaaggtg agcgccataa tgcacttgat
      181 gacgcccgtt accaggcaaa atacgtttca gttatctggc aaaaactgat cccgagtcag
      241 gctgattctt aatgttcaac tgtcgccggt tgtgactggt attctgcaac cggcgctcgt
      301 ctgatgtaag agataaagaa atcgatgagc gaagtaatca tgattgtctc tcccggcaaa
      361 tgggtatccg aagagcagtt aattgcgctg aaaggaataa aaaaaggtac gttaaaaaag
      421 gcccgggaaa aatcgtttat ggaaggaagg gaatataagc atgtcgctca tgacggtatg
      481 ccatgggata acagtccatg cttttacaac ctggaagaaa ttgatcgctg gattgagcgc
      541 caggcatcag cgagaccaag acgtcatctt acttgactaa aagccacact aactaatgag
      601 agaagttgaa atgaaatatc cgacaggcgt ggaaaaccat ggagggaaat tacgtatctg
      661 gtttgtttat aaagacgtaa gagtcaggga aaatctgggg gttcctgaca cagcaaaaaa
      721 caggcgcgtt gcaggtgaac tacgctcctc tgtttgttac gcaataaaaa ctggtgtttt
      781 cgactatgca aaacagtttc cctcctcacg caatctggaa aaatttggtg aggcccgaca
      841 agatttaacc ataaaagaac tggctgaaaa atttctggca ctgaaagaaa ctgaagtcgc
      901 caaaacatca ctcaacacat accgtgccgt catcaaaaat atcctgagca taatcggtga
      961 aaaaaatctt gcctcatcga ttaataaaga aaaattactg gaggttcgta aagagttact
     1021 gactggatac cagatcccca aaagtaacta tattgttaca caaccaggga gatcggctgt
     1081 aactgtaaat aattacatga caaatcttaa cgccgtgttc cagtttggtg ttgataacgg
     1141 ttacctggca gataatccgt ttaaggggat ctcgccatta aaggaatcaa gaaccattcc
     1201 ggatcctctt tcgcgggaag aatttatccg tcttatcgat gcgtgcagaa atcagcaagc
     1261 aaaaaattta tggtgtgttt ctgtttatac tggagttcgc cctggtgagc tgtgtgcact
     1321 tggatgggag gacatagatc tgaaaaatgg aacaatgatg atcaggagaa atttagcaaa
     1381 agaccgtttc acggtaccaa aaacacaggc gggaaccaat cgggtcattc atcttattaa
     1441 gccagcaatc gacgctctcc ggagtcagat gacattaacg agactgagca aagagcatat
     1501 cattgatgtt cacctcagag agtatggcag aacagaaaaa caaaaatgca cctttgtttt
     1561 tcaacctgaa gtgtcagcga gagtaaaaaa ttatggtgac cattttaccg ttgactcaat
     1621 aaggcagatg tgggacgcag cgataaaacg tgccggactc cgccatcgaa aatcatatca
     1681 gtcgagacat acttatgcct gctggtcgct gacagctggt gctaacccgg catttatagc
     1741 aaaccagatg ggccatgcag atgcgcaaat ggtatttcag gtatacggaa aatggatgtc
     1801 tgaaaacaat aatgcacagg tagctttgtt aaatacacag ttaagcgagt ttgccccaac
     1861 catgccccat aacgaagcaa tgaaaaatta atttaatatt tatcaaatag ttaacacgca
     1921 tgactcttga aatccataaa ttcaagcgca gtgcccagcc atcccgatac tgctgctttc
     1981 accaaatcct tagtgcttct ttcgtgtttt tctattgtca taatggttat ctctaaaaaa
     2041 gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa agtgagtaag
     2101 actttgcagc aatgtttttg atcctgttca aataaactaa tggcatcagc aacatgctgg
     2161 aaatcaaacg tatgggtaat taatttttct ggtttaatta accctttact taaccagtcg
     2221 ataacgaccg gaaatttatt tgcatttaag cgtgaagaaa aaatagagag ttcttttccg
     2281 gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa tacaatacgt
     2341 gccgctggag aagccagcgt tacggcctct ttcaggatag aaggatgaca agccgcatcg
     2401 ataattaatg tcggcttgat gcctttttca gtgaaaatct cgccaagcgg tgtctggctg
     2461 ttattaatcg cccagtcagc cccgctctct ttcgcttttt ccagtcgttc atcaatgcga
     2521 tcggcaacaa tcacattttt aacgttatag acgcctttta atacctgaac gatcgtcagg
     2581 ccgattggac cggcaccata aaccagaacg gtatcatttt cagtcggttg accatgaccg
     2641 gttacgttag ccgcaatggt aaaaggttcg atcatcaccg catattgatc ggccactgct
     2701 tcaggaattt tccacgcatt ttttgccgga accacggcat attcactgaa accaccgtca
     2761 gcgtgcacac ctaatacagc cagtgtcgta caaacgttcg gtttacctat agagcacgga
     2821 tagcaatgcc cacagctgac caccggatcg acagcaacac gttcaccgac tctggcgctt
     2881 tccacgccgt cacccactgc atcaatgacg ccaaagaatt catgaccaat gacgcgcgga
     2941 tatttcgcaa aaggattatg cccacgataa atatggctat ctgaaccaca aattccggca
     3001 agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcacg ttcgacaatc
     3061 gccagttgat tcggtttttc aattaatatg cttttcatta tcttactcct taccagttcc
     3121 acagcgtgcc atcttccaga cgtgcgactg gtagataagc aggttcatag ggatatttcg
     3181 ccgccagctt ttcatcgaat tcgataccaa gacccggttt gtctcccgga tgcatatagc
     3241 cgttatcgaa agtccagttg tgcgggaaga cttcgagcat ttgttcggaa taacccatgt
     3301 attcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga
     3361 ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat
     3421 cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg cggatataat
     3481 cgatgagttg ctcttcaatc agttgtttgc agtcccagat gctgttgaag acttcaccca
     3541 ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcctgg ttttccgcag
     3601 gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg
     3661 cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta aaaccaaact
     3721 tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca
     3781 gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct ttacctttcg
     3841 acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc
     3901 ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga ccggtggtat
     3961 ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt
     4021 tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg
     4081 taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac
     4141 ggtgcgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa gaggccacgg
     4201 aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga
     4261 tttttaacgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct
     4321 tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa ctgaactact
     4381 accatacaag tatgaagatc gaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg
     4441 cgctgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga cacttcctcg
     4501 tatcttattg ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc aacaatgatc
     4561 aacatgccgc aaagcgcaat caacgccccc gtccagtcat aaagacttag tttcacgcca
     4621 tccacaacgc gcagccacat caacgccgtg cagacataaa cgccaccata agccgcgtaa
     4681 acacgcccac tcgccgcagg atgcaacgtt aacaaccaaa caaacagcgc cagtgaaatc
     4741 cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca gggcagaaag
     4801 catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt
     4861 gtcacttatt gacatcatgt atagttatag ggcgacataa tatcatcaat ataaacaccc
     4921 tcctggtacg ttttgcgttc gcagtggatg gtgtagaatc acctttaatc attcatacag
     4981 ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc
     5041 ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg tgattgagtc
     5101 tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat ggaatgacac
     5161 gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc
     5221 cgcttttgat aaccgagata aatgtgagca aagcgccaac atcaatgcct actgggagcc
     5281 aaatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct aacctgttat
     5341 tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag ctacgcccgc
     5401 tggagcgtga agatttacgc tatgtacatc aactcgacaa taacgccagt gtgatgcgtt
     5461 actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtat gataagcata
     5521 ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg
     5581 tggagctggt ggaaattaac catgtacatc gccgcgcaga atttcagata attatctccc
     5641 cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg gactatggct
     5701 tcaccgttct caatctctat aagttgtatc tgatcgtcga taaagagaat gaaaaagcga
     5761 ttcacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat gagtttttta
     5821 ttaatggtca atatcgtaat gccattcgca tgtgtatatt ccagcatcag tatctggcag
     5881 agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat taataataat
     5941 cgattgtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct tcatcaacaa
     6001 tgatcagctg acagtccacc ggattggcgt gactgtcata ttcacagctc tgttttacat
     6061 taccaactcg ttgattattc agtaaagtaa ccgctgtgta atctaatttt ttgatcgggt
     6121 ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgtg gttttaccca
     6181 gtggataacc ctgatcatca tagcgatatt ccatctgcat ttgtttgctg ctggctttaa
     6241 tcacgaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat tctgctagct
     6301 ggcattttcc ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg taataattgg
     6361 cgtccagtac cagagcgacc acggtattat tttccagatc cagtaattcg agtgaatcaa
     6421 aacagccttc ttgcgacaaa gtcccagaaa cacgtttcgt cacttcacct tgctcatcca
     6481 ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg aattcattag
     6541 agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga
     6601 ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac aacagtcacc agagagtaga
     6661 gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag
     6721 aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg
     6781 ctcgccctcg cctcacccgc agtactcgcc gcgccgggat cctgtgagcg catacagagc
     6841 gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac attaagtatt
     6901 gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg tgctaatgat
     6961 acgcataaaa ttctctacac ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct
     7021 acccaggatg gcgcgcctgc cgaaccgcag taatacatta tcgactgaac gccggatacg
     7081 acacatccgg cgatttgaac atacaacata atcccacctt attactcata cccttctatt
     7141 gatatggatt aataattctt aacccaaaat gggtagactc cctctattgt tagcgcgcta
     7201 aatattcaat atataaactt ttatataacg ataaagaaca gggagtgagt tatgtccaaa
     7261 aatgaacgaa tggtgggcat cagccgcaga acactcgtta aatctaccgc gataggttct
     7321 ctggcgctgg ctgcaggcgg tttttctttg ccgtttaccc tgcgcagtgc agcagcagcg
     7381 gtacaacagg cccgcgaaaa agtggtctgg ggtgcctgtt ccgtcaactg tggtagccgc
     7441 tgtgcacttc gtctacatgt taaagataat gaagtgacct gggtggaaac tgacaatacc
     7501 ggcagcgatg agtacggcaa ccatcaggta cgcgcctgtt tgcgcggtcg ctccatccgc
     7561 cggcgtatta atcatcccga tcgcttgaat tacccaatga aacgcgtggg taaacgcggt
     7621 gaaggcaaat tcgaacggat tagctgggat gaagccctgg atactatcgc cagtagcctg
     7681 aaaaaaaccg tcgaacaata tggcaatgag gctgtatata ttcagtactc ttcggggatt
     7741 gttggcggca atatgacccg ctcttcgcca tcagcctcgg cggtcaaacg cctgatgaac
     7801 tgctacggcg gttcactcaa tcagtatggc tcctacagca ccgcgcaaat ttcctgtgcc
     7861 atgccctaca cctacggcag taatgatggc aacagcacca cggatattga aaacagcaag
     7921 ctggtcgtga tgtttggtaa caacccggca gaaacccgca tgagcggcgg tggcatcact
     7981 tatcttcttg aaaaagcgcg cgagaaatcg aacgccaaaa tgattgttat cgatccgcga
     8041 tataccgata cggctgctgg tcgtgaagat gaatggcttc ctattcgccc gggcaccgat
     8101 gccgcgctgg ttgcggggat tgcctgggta ttgattaacg aaaatctcgt tgatcaacct
     8161 tttctcgata aatactgcgt cggctatgac gaaaaaacct tacccgcaga tgcaccaaaa
     8221 aatggtcact ataaagccta tattcttggt gaaggtgacg ataaaacagc gaaaacgccg
     8281 cagtgggctt cgcaaattac cggtatcccg gtagaccgta tcatcaaact ggcgcgtgaa
     8341 attggcacag caaaacccgc ttatatctgc cagggctggg ggccgcaacg ccaggcaaac
     8401 ggcgagctga ctgcacgcgc tattgctatg ttgcctattt tgacgggcaa tgtcggcatc
     8461 agcggcggaa atagtggcgc gcgtgagtcg acctatacca ttaccataga gcgcctgccg
     8521 gtgctggata atccggtcaa aacgtcaatc tcctgcttca gctggacaga tgctatcgat
     8581 catggtccgc aaatgacggc aatccgcgac ggcgtccgcg gcaaagataa actggatgtg
     8641 cccattaagt tcatctggaa ctacgcggga aataccctgg ttaatcagca ttctgacatc
     8701 aacaaaacgc atgaaattct gcaagacgaa tcgaaatgcg aaatgattgt ggtcatcgaa
     8761 aactttatga cctcatcggc aaaatatgcc gacattctgc tgccagacct gatgaccgtt
     8821 gagcaggaag atattattcc taacgactac gccggtaaca tgggatatct cattttcctc
     8881 cagccagtca ccagcgaaaa attcgaacgt aaaccgattt actggatcct gagtgaagtc
     8941 gcgaaacgtc ttggacctga cgtctatcaa aagttcacag aaggtcgcac gcaggaacaa
     9001 tggttacaac atctgtacgc caaaatgctt gccaaagatc cggcgttacc gtcttacgac
     9061 gaactgaaaa aaatgggtat ctataagtgt aaagatccca atggtcattt tgtcgcctac
     9121 aaagcatttc gtgacgaccc cgaggcaaat ccacttaaaa cgccttccgg taagattgaa
     9181 atttattcca gcaggctggc ggaaattgcc cgtacctggg aactggaaaa agatgaagtg
     9241 ataagcccat tgccagttta tgcctcaacc tttgaaggct gggactcccc tgaacgtaga
     9301 accttcccac tgcaactgtt tggtttccat tacaaatccc gtactcactc gacctacggc
     9361 aatattgatc tcttgaaggc tgcctgccgt caggaggtgt ggatcaaccc tatagatgcg
     9421 cagaaacgtg ggattgccaa cggcgatatg gttcgggtgt ttaaccatcg tggcgaagtt
     9481 cggctaccag ccaaagtaac accacgcatt ctccctggag ttagcgctat gggccaggga
     9541 gcctggcacg aggccaatat gtctggcgat aaaatcgacc atggaggctg tgtgaatacg
     9601 ctaaccactc tgcgcccttc accactggcg aagggaaacc cgcagcacac taatctggtc
     9661 gagatcgaaa aaatataacc cacgacaacc ataaattctg gcatgacatt ttgtttgaaa
     9721 agcaataagt gagtaatgat gaaaatccat accaaagagg cgctcatgaa ggctgaaatc
     9781 agccgtagaa gtctgatgaa aacctccgca cttggcagtc ttgcgctggc aagcagtgct
     9841 ttcactctgc cattttccca aatggtccgg gcggcagagg ctccggtaga agagaaagcg
     9901 gtctggagtt cctgcaccgt taactgcggg agccgctgtc tgttacgttt gcatgtgaaa
     9961 gatgacaccg tgtactgggt ggagtctgat acgacaggtg acgacgtcta cggtaatcat
    10021 caggttcgag cgtgtttacg cggacgctct atccgccgac ggatgaatca tcctgatagg
    10081 ttgaaatatc ccatgaagcg cgtcggcaag cgcggtgaag gtaaatttga acggataagt
    10141 tgggacgaag ccctggatac catcagtgat aatcttcggc ggatcctaaa agattacggc
    10201 aatgaggctg tacatgttct ctatggaaca ggcgtagatg gtggaaacat caccaactca
    10261 aacgtcccgt accgtctgat gaactcttgt ggtggttttc tcagtcgcta tggcagctac
    10321 agtaccgcac agatcagtgc cgcaatgagt tatatgttcg gtgccaatga tggcaacagc
    10381 ccggatgata tcgccaatac gaagctggtc gttatgttcg gaaataaccc ggcagaaacg
    10441 cggatgagcg gcggtggtgt cacttactac gtcgagcaag cccgcgaacg ttcaaacgca
    10501 cgcatgatcg tcatcgatcc acgttataac gacacagctg ccggacgtga agatgaatgg
    10561 ctacccattc gccctggcac cgatggcgca ctggcctgtg cgattgcctg ggtactgatt
    10621 acggaaaaca tggtcgatca gccatttctc gacaaatatt gtgttggtta cgatgaaaaa
    10681 acgctgcccg ccaacgcacc acgtaacgcg cattataaat gcctatattc tgggcgaagg
    10741 gcctgacggc atagctaaaa cgccggaatg ggcagcaaaa atcaccagca taccggcaga
    10801 aaaaattatc cagttggcac gagagatcgg ttcagcaaaa cctgcttata tttgtcaggg
    10861 ttgggggcca caacgacact ccaacggcga acaaacgtcc cgcgctattg ccatgctttc
    10921 cgttctcacc ggtaacgtcg gcataaacgg cggcaactca ggtgtacgcg aaggtagctg
    10981 ggatctgggg gtagaatggt tcccgatgct tgagaatcct gttaaaacgc agatttccgt
    11041 ctttacatgg acagatgcca tcgaccacgg tacggaaatg accgcgaccc gtgatggtgt
    11101 tcgtggaaaa gaaaaactgg atgtccccat caagttttta tggtgctacg ccagtaacac
    11161 attgatcaat caacatggcg acatcaatca cacccatgag gtgcttcagg atgacagcaa
    11221 gtgcgagatg attgttggca ttgaccattt tatgaccgcc tcagcaaaat attgcgatat
    11281 cctgctgccc gacctgatgc caacagagca agaagacctc atctctcatg agtctgccgg
    11341 gaatatgggc tatgtgatcc tcgcccaacc cgcaacctcg gcaaaattcg aacgcaaacc
    11401 tatctactgg atgttgagtg aaattgcgaa acgtttagga ccagacgttt atcaaacctt
    11461 cactgaaggt cgtagccaac atgaatggat caaatacctt catgcgaaaa cgaaggaacg
    11521 taatccggaa atgcccgact acgaggagat gaaaacgacc gggatcttta agaaaaaatg
    11581 cccggaagaa cactacgtcg ctttccgcgc attccgtgaa gatccccagg caaacccgtt
    11641 gaaaacacct tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga ttgcggatac
    11701 ctgggaattg aaaaaagatg aaattattca tccccttcca gcttatacgc caggttttga
    11761 tggctgggac gatcccctgc ggaaaaccta tccactgcag ttaacgggct tccactacaa
    11821 agcgcgtacc cactccagct acggcaatat tgatgtgtta cagcaggcct gcccacaaga
    11881 ggtgtggatc aaccccattg atgctcaggc acgcggtatc cgtcatggcg ataccgtgcg
    11941 ggtatttaac aataatggag aaatgctgat tgccgcaaaa gtgactccgc gtattctgcc
    12001 tggcgttacc gccatcgggc aaggtgcttg gcttaaagcg gatatgtttg gtgaccgggt
    12061 cgatcacggc ggcagtatca atattctgac ctcgcaccgc ccttcaccgc tggcaaaggg
    12121 aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt taaggagtag cccatgacca
    12181 cacaatatgg attttttatc gattccagcc gctgtactgg ctgtaaaacc tgcgaactgg
    12241 cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt ccgccgtatt tatgaatatg
    12301 ctggcggcga ctggcaggaa gataatggcg tctggcacca gaacgtgttc gcttattatc
    12361 tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc cccagcggcg
    12421 caatgcataa gcgtgaagat ggctttgtgg tggttgatga agatgtttgt atcggctgcc
    12481 gctactgcca catggcttgt ccgtacggcg caccacagta caatgctgaa aaagggcaca
    12541 tgacgaagtg cgatggttgt tattcgcgtg tcgccgaggg gaaacaaccc atatgcgtcg
    12601 aatcctgccc gctgcgtgca cttgagttcg gtccaattga agagctgcgc cagaagcacg
    12661 gcactctggc tgccgtcgcg ccactgccgc gcgcgcattt cacaaaaccc aatatcgtta
    12721 tcaaacctaa cgccaacagc cgcccgaccg gcgataccac tggttatctg gctaatccgg
    12781 aggaagtgta aaatgggaaa tggatggcat gaatggccac tggttatctt taccgtattg
    12841 ggtcaatgcg tagtaggcgc gctgattgtt agcggaattg gctggttcgc cgccaaaaat
    12901 gacgcagacc gtcagcacat tgttcgtggc atgtttttcc tctggctgtt gatgggcatc
    12961 gggtttattg cctctgtcat gcacctggga tcgccactgc gggcgtttaa ctcgctgaat
    13021 cgaatcggag cttctggtct gagtaacgag attgctgccg gttccatatt ttttgccgtt
    13081 ggcggactat ggtggctggt ggcggtcatc ggtaaaatgc cgcaagcgtt ggggaaactc
    13141 tggttacttg tcagcatggc gctcggcgtc attttcgtct ggatgatgac ctgcgtgtat
    13201 caaatcgaca ctgtgccaac ctggcataac ggttacacta cgctggcttt cttcctgacg
    13261 gtattgttga gtggtccaat acttgctgcc gcaattctgc gggcagcccg cgttacattt
    13321 aataccacac catttgccat cattagcgtt ctggcgctga tcgcttgtgc cggggtgatt
    13381 gtgctgcaag gtttgtctct gtcgtctatt cactcatccg tgcaacaagc cagcgcactg
    13441 gtaccagatt acgcctcctt acaggtctgg cgtgtggtat tgttatgcgc aggtcttgga
    13501 tgctggcttt gtccgctcat tcgtcgccgt gaacctcacg ttgccggact tattctgggg
    13561 ctaatcctca tacttggcgg tgagatgatc gggcgtgtgc tcttttatgg cctgcacatg
    13621 acagtaggaa tggctattgc aggttaacaa attggtgcgg ggcaacccgc acatttcagg
    13681 atgttaggaa tgacccattt ttcacagcaa gataattttt ctgtcgcggc gcgcgtgttg
    13741 ggcgcgctgt tttattacgc tccagagagt gcggaagccg cacctctggt tgcggtactc
    13801 accagtgacg gctgggaaac tcagtggcct ctaccagaag cgtcattagc gccgctggtg
    13861 acggcttttc agacacagtg tgaagagact cacgcccagg cctggcagcg tttgtttgtc
    13921 ggcccgtggg cactgccgtc tccgccgtgg gggtcggtct ggctggatcg cgaatctgtg
    13981 ctgtttggcg attcaacatt ggcacttcgt cagtggatgc gcgagaaagg cattcagttt
    14041 gaaatgaagc aaaacgaacc ggaagatcat tttggttcac tgctgttgat ggctgcgtgg
    14101 ttggcggaga atggtcgcca gacagaatgc gaagaattgc tggcatggca cctttttccg
    14161 tggtcaacac gttttcttga tgtttttatc gaaaaagcag aacacccttt ctaccgtgca
    14221 ctgggtgaac tggctcgctt aacgctggcg caatggcaat cacaactgtt aattcctgtc
    14281 gcggttaaac cgctgtttcg ataggatcac cgtaatattg ccggatggtg acgattacgc
    14341 gcaatccggc aataataggt tacagtgtca cgttttttta tctcttaaag cacgcactgc
    14401 ttttgcggct ggcctctttt gccgcaaaat agtcgcccgt gtttcattgc ccatttctgc
    14461 tcatgcatca tctacacatc tatccggatc tgcgcactat gttccgccgt ctgcttatcg
    14521 caacagtcgt cggtattctc gcggcctttg ccgttgccgg gtttcgtcat gcgatgctgc
    14581 tactggagtg gttgttcctc aataatgact ccggcagtct ggtcaatgca gcgacaaacc
    14641 tttccccctg gcgacggttg ctaactccgg cgctcggcgg actggcggcg ggtttgttgc
    14701 tgatgggctg gcagaaattt acccaacaac gccctcatgc gccgaccgat tatatggaag
    14761 cgttgcaaac cgatggacag ttcgattacg cagcaagcct ggttaaatcg cttgcctctc
    14821 tgctggtagt aaccagcggc agtgcaattg gtcgcgaagg tgcgatgatt cttttagctg
    14881 cccttgccgc ctcctgtttt gcccaacgtt ttacgccacg ccaggagtgg aaattatgga
    14941 tcgcctgtgg ggccgcggcg ggaatggctg cggcctatcg tgccccgctt gctggcagtt
    15001 tatttatagc cgaagtgctg tttggcacta tgatgttggc ctctctcggc ccggtgatta
    15061 tttccgccgt cgtggcattg ctggttagca atctgattaa tcatagcgac gcgttactct
    15121 acaacgtaca actctcagtg acggttcagg ctcgtgacta tgcgctgatt atcagtacag
    15181 gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt aatgaacgcc tgtcatcgtg
    15241 gatttgtgag tctcaaactt gcgccgccct ggcaactggc actaggcggg ttgatcgtgg
    15301 gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg ctatagcacc gtacaatcct
    15361 ttttaaccgc cccaccactg ttaatgatca ttgccgggat cttcctttgt aaactgtgtg
    15421 ccgtgctggc gagtagcggt tccggcgcac ccggtggggt ctttacaccg acgctattta
    15481 tcggtcttgc cattggcatg ttgtatggtc gtagcctggg attatggttc cctgatggcg
    15541 aagaaattac acttttactc ggattgaccg ggatggcgac actgttggcg gcgaccacgc
    15601 acgcgccgat tatgtcgacg ttgatgatat gtgaaatgac cggggagtat cagctactcc
    15661 ccggtttatt gattgcctgc gtaattgcgt cggtaatttc gcggacgtta caccgtgact
    15721 ctatctaccg ccagcacact gcgcagcata gctaagcgga tgtattgccc cagttcacgc
    15781 tgttcggcgc gcggcagata aggcagttca ccaatgagcg gtgccggaag ttttttaccc
    15841 agcacatcaa tgatttccgc ataatgcgcc agtcctgggt tgattcggtt agccacccag
    15901 ccaatgagcg gcagcccgtc gttggcgatc gcctgagctg ttagcagtgc atggttaatg
    15961 caaccttcct gaataccgac aaccatcaac accggcagtt gttcctgcac tacccattca
    16021 gagagtggac gcaaatcatt catcagactg cgccagccgc cagtcccttc taccacgaca
    16081 tgatcgactt tttcggtcag gtttgccagg ccgtttgaaa tgagggtgta attgattggg
    16141 caactgtgcg ccacgctact ttcttcttcg cttaacgcga taggattaac tgcttcataa
    16201 ggcagttcga tggttgaaac actctgcaac accagggcat ctttattacg cagcccttcg
    16261 ggtgtctctt tgctcccctt cgctacgggt ttatatcccg caaccgtttt tccctgggag
    16321 gctaacgctt gtagcaatgc gcgggaaacc accgttttcc ctacagaagt gtctgtaccg
    16381 gtaataaaga aacgcttcag catcactaac tccaccgtta tgcttcacaa atataaacca
    16441 ggaaaataat taaccttgaa agtctaagtt atgctttcct ggcccaaatt gagatagcgc
    16501 aaattttggt agaacagtta aaaaatgtta accctgcaac agacgaatca acaaagaacc
    16561 gttatacatc gcgtctttta ccagtgcagc gcctgccatc gtgccctggt tagaaaactg
    16621 agtactctca acgctgatgt gctgactata cgcaggaagg gcctgctgac ggatgctgtc
    16681 tgagatgacc gggaagagga tatctgccgc tttacttaac ggtgagccaa tcagtatttt
    16741 ttgtgggtta aataaattca ccatgatggc aagaatgcgc ccgacatgcg cgcccacccc
    16801 ggtaatgatg tcttttgcca gtagatcgcc gcgcaatgcc gcctgacaca atgagtccac
    16861 ggttaacggt tgtccatgta acatcgagct catggattga ttaagacgca gctgtgccag
    16921 ctcaagaata ctgtccacgc tggcgatggt ttcgaggcag ccgtgattcc cgcaataaca
    16981 gcgtttccca tacgggtcga cctgtgtgtg gcctatttcc acgagactac tgctgcctgc
    17041 gtgtagcaga tgaccatcgg taatgacgcc cgcccccacg ttgtgatcga taaccacctg
    17101 aatcacatcg cgcgccccgc gtgaggcacc aaacaaggcc tctgccatcg tccatgcgct
    17161 gatatcatgc tgaatataaa ccggaacgcc ggtatgctgc tccagcgcct cgccgagcgg
    17221 catctctttt acatcctcgt agaacggcat gcgatgtaca ataccatttt ccgtatcaat
    17281 aattcccggc aaggttatgg caatcgaagt tagacgctca agttttttct ggtggcggat
    17341 aaaaaactga tcgatatggg aaataatacg atccagcaat ggcaagtcat cttttaacgc
    17401 cagttcctgc gactcttcca ccaccagttt gctgctcaga tcgcgcagag caaggaaaat
    17461 ctccccgcga ctaatgcgca gagaaagata gtgccaggct tcagtttcaa ccaccagccc
    17521 caccgccgga cggccacggt tccccgcttc tttgatttcc agctcttgca ccaggtgtgc
    17581 ttcgagcatc tcacggacaa ttttagtgat actggcagga gccagttgcg ccagacggga
    17641 aagatcgata cgcgagactg gaccaagctg atcaatcagg cgataaaccg cgcccgcgtt
    17701 ggtctgcttt atttgatcaa tgtgcccagg ctggttttca gcaaccaccg catactccct
    17761 atattttcgc gctccgaaat aatctgtagg ctatggtgaa gcacttcaat acgtgtcgtc
    17821 aaatttttac ttaggcatgt gattaacagc acatttttcg ggctttttcg ctgaaatttc
    17881 cctcacctga gagcattcag cagatgaata cgaaagttac gcgcagccgg actttgttca
    17941 tgatgtttcg gccagaccaa ccacatttca gaaaccgcat cctcttcagc aatcggcacc
    18001 cagcgcattt cgttgagctg aacacgttta aatgacgcag gcaaaattga aacacccaga
    18061 ccggcggaaa ccagaccgat gatggtcatt gcctcgccca cctcctgagt gatgacgggc
    18121 gtcaaatggt aacgtcgcat cagcccgaga atatcgtcat acagccctgt cccgacgtgc
    18181 ggatcaaaaa agacaaaggg ttctttcgcc agttcagcca gcgttacatt cgggttattt
    18241 gccaggggat gatcgtgcgg gatcatcgcc ataagcggtt catggacgat gactgcgtgt
    18301 tcaagcgact ccggtaacgc tgtgttacgc agcaatccca tatccagcgt tccttcaatg
    18361 agcggagcga tttgctcgcg agtgttcatt tcgcgggttt gtaaatggac atcaggataa
    18421 tcacggcgaa acagcgataa cgtatcggac accgcccgaa taaaaggagc cgacgaagta
    18481 aaaccaatgc gcaactcccc cgcttcaccc tgatgcagcc tttcagcgcg agcggcagcg
    18541 tcatccacca tagacaggat ttgccgacta tctgcaagaa actgttttcc tgctgccgtc
    18601 agcaatacac tgcgattggt tcgtgccagc agtcgggcac caatttgttg ctccagcgcc
    18661 tgaatctgct gacttagcgg cggttgcgaa atattcaggc gggcagcggc gcgcccgaaa
    18721 tgcagctctt ccgcaacagc aacaaagtaa cgcagatgac gaagttcaat attcatattt
    18781 aaaacatctt atttgagatt attaatatat tagacagaac aattcgattt tcctacccta
    18841 tgtataagcc tgatctacag gcatatttag caaggatttc aagtgagccg tactacaact
    18901 gttgatggcg ctccggcaag cgacactgac aagcaaagca tttctcagcc aaatcaattt
    18961 attaaacgcg gtacgccgca atttatgcgc gtcaccctgg cgctgttctc tgccggactg
    19021 gcaacatttg cacttctcta ttgtgtgcag cctatccttc cggtgctttc gcaggagttt
    19081 ggcttaaccc ccgcgaacag tagtatttca ctgtccattt ccacggcgat gttggctatt
    19141 ggtttgctgt ttactggccc gctatccgat gccattggtc gcaaaccagt gatggtcacg
    19201 gcgctactgt tggcctccat ttgtacgtta ctttcgacaa tgatgaccag ctggcacggc
    19261 attttgatta tgcgcgcctt gattgggctt tcgttaagtg gcgtggcagc tgttggcatg
    19321 acttatctta gcgaggaaat ccatcccagt ttcgtggcct tttcaatggg gttgtatatc
    19381 agcggcaact caattggcgg catgagcgga cgcttaatta gcggtgtctt cacggacttt
    19441 ttcaactggc gaattgctct ggcggcaatc ggttgtttcg cgctggcctc ggcgttgatg
    19501 ttctggaaaa tcctccctga atcacgccat tttcgcccga cttcgctgcg ccctaagacg
    19561 ttgtttatca actttcgtct gcactggcgt gaccggggat taccgttatt gttcgcagaa
    19621 ggctttttgc tgatggggtc gttcgtcacg ctgtttaatt acatcggcta tcggttgatg
    19681 ctctccccct ggcatgtcag tcaggccgtg gttggcttat tatcgctggc ttatttgacc
    19741 ggtacatgga gctcacccaa agccggaacc atgaccaccc gctatgggcg tggtccagtg
    19801 atgttgtttt cgacgggggt tatgctgttt ggtttactga tgaccttatt cagctcgctg
    19861 tggctgatct ttgccggaat gttactcttc tcagcaggat tcttcgcagc ccactcagta
    19921 gccagcagct ggatcggccc ccgcgcaaaa cgcgctaaag gccaggcctc ctcgctgtat
    19981 ctgttcagtt actatctggg gtcgagtatt gccgggacgc tgggtggtgt tttctggcat
    20041 aactatggct ggaacggcgt cggcgcattt attgctctga tgctggtcat tgctctgctg
    20101 gtcgggacgc gtttgcatcg tcgtctgcac gcctgaaaaa taagtccgga ctgcggtaaa
    20161 tacccgtccg gacttattgc cagctcaaac caacgttaat agccatccta aaatagacga
    20221 agcgccagcc aattcccgca gcgcgtctag cgtcatcagg attataagta cccaaataaa
    20281 cggattcatt ttgctgtgtg tcatttatta ctgatgcgca gttattctac tgctttgtaa
    20341 gtagtaaaat agttaacccg atcaagacta ctattattgg tagctaaatt tcccttaagt
    20401 cacaatacgt tattatcaac gctgtaattt attcagcgtt tgtacatatc gttacacgct
    20461 gaaaccaacc actcacggaa gtctgccatt cccagggata tagttatttc aacggccccg
    20521 cagtggggtt aaatgaaaaa acaaattgag ggtatgacaa tgaaaaaagt attagctctg
    20581 gttgttgccg ctgctatggg tctgtcttct gccgcctttg ctgcagagac tacgaccaca
    20641 cctgctccga ctgcgacgac caccaaagca gcgccggcga aaactataca tcataaaaaa
    20701 cagcataaag cagcacctgc ccagaaagcg caggcggcta aaaagcatca taaaaatacg
    20761 aaagctgaac agaaagcccc tgaacaaaaa gcgcaggcag cgaagaaaca cgccaagaaa
    20821 cacagccatc agcaaccggc aaaatctgct gcacaacccg cagcgtaagt tttcaacagt
    20881 aatgctggcg cgccccctcg cgcctgaaaa ttacggtgct aagcgggtaa cgtttagcac
    20941 cgcctttagc ggagggtagt atggtgggcc gttatcgctt tgagttcatt ctgatcatcc
    21001 ttattttatg cgcactgatt gccgcccgtt tttatctttc ctgattgtag ttatctgatt
    21061 ttactcccac tttcatcccg tcccgtctat agtatttatg agggtttgct tttaataatc
    21121 ataattaccc accagagtgt gatatgcgta caaccattgc tgtagtgttg ggtgcaatta
    21181 gtttgacgtc tgcttttgtg tttgcagata aaccagacgt tgccagatcg gcaaacgatg
    21241 aggtcagcac cctgtttttt ggtcatgatg atcgtgtgcc agtgaatgac acgacccaat
    21301 caccgtggga tgcggttggg caactggaaa cggccagcgg caatttatgt acggcgacgc
    21361 tgattgcacc caatctggca ttaacggcag gacactgttt attgacacct ccaaagggta
    21421 aagccgataa agcagtggcg ctgcgttttg tgtcaaataa aggtctttgg cgctatgaga
    21481 tccacgacat agaaggccgc gttgatccga cactgggaaa gcggttaaaa gcagatgggg
    21541 atggttggat tgtacctccc gcagccgcgc cgtgggactt cggattgatt gtgctacgta
    21601 atcccccttc tggcattacg ccgttgccgt tatttgaggg agataaagcc gcgcttaccg
    21661 ccgcattaaa agcggcaggt cgtaaagtga ctcaggcagg ctaccctgaa gatcatctcg
    21721 atacgttgta cagtcatcaa aactgtgaag tgactggctg ggcgcaaacg tcggtgatgt
    21781 cacatcagtg cgataccttg ccgggtgaca gcggttcgcc tctgatgttg cataccgatg
    21841 acggctggca attaattggg gtgcaaagtt cggctcctgc cgcgaaagat cgctggcgcg
    21901 ccgataaccg ggccatttct gttaccggtt ttcgcgacaa gctggatcaa ctgtcgcaaa
    21961 aataatgttc aaacgctgcc cgacagcgcg ggcagcgtct tcatcaggca agtttcacca
    22021 tgatcattcc agccaacagc aagaccaggc caatccagcc tttacgattt aaccgttgac
    22081 caaacaagat ccaacctgcg gctaacgtgg cggcaatacc aaacccgccc cacaatgcat
    22141 aagcgacaga caagtcgatc cctttaacgg cttgagaaag cgcactaaag gcagccagca
    22201 ccgccgccag ggagagcaag ccaaatattt tgcgacgaaa gccgtcagaa aatttcaaaa
    22261 agacgttagc aacgatttcc agcacgattg ccaatgccag ccaggcggcg tgaacccatt
    22321 caaactgcgc catggttcac ctccagttca ggtttacgcg ctttacgggt acctgatttt
    22381 atcaacacaa tcccggcgac cagggtggtt aacccggcaa ttttcatcag cgataaactt
    22441 tcgtcgaata acaaaacgct aaacaaggta ataaataaaa taccgatacc ttcccacagc
    22501 gcataagcta cgcctaaggc gattttttta acggcgaaag agagaaatat atacgacaga
    22561 gaaatcatca ccagcattaa aataaagccg ccatttccct cactgacgct cgcccatttc
    22621 attgacagcg taccggtaat ttctgtagc
//
LOCUS       DAZFZC010000083        22393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFZC010000083 DAZFZC010000000
VERSION     DAZFZC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.083"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            213..1112
                     /gene="yegS"
                     /locus_tag="ACHKXP_003624"
     CDS             213..1112
                     /gene="yegS"
                     /locus_tag="ACHKXP_003624"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO7648555.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSRFDIQAPHDITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1194..1967)
                     /locus_tag="ACHKXP_003625"
     CDS             complement(1194..1967)
                     /locus_tag="ACHKXP_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7648556.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2073..3113)
                     /gene="gatD"
                     /locus_tag="ACHKXP_003626"
     CDS             complement(2073..3113)
                     /gene="gatD"
                     /locus_tag="ACHKXP_003626"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7648557.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3161..4516)
                     /locus_tag="ACHKXP_003627"
     CDS             complement(3161..4516)
                     /locus_tag="ACHKXP_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO7648558.1"
                     /translation="MFSEVMRYILDLSPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4520..4804)
                     /gene="gatB"
                     /locus_tag="ACHKXP_003628"
     CDS             complement(4520..4804)
                     /gene="gatB"
                     /locus_tag="ACHKXP_003628"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO7648559.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4835..5287)
                     /gene="gatA"
                     /locus_tag="ACHKXP_003629"
     CDS             complement(4835..5287)
                     /gene="gatA"
                     /locus_tag="ACHKXP_003629"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO7648560.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5297..6559)
                     /gene="gatZ"
                     /locus_tag="ACHKXP_003630"
     CDS             complement(5297..6559)
                     /gene="gatZ"
                     /locus_tag="ACHKXP_003630"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO7648561.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADVA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6588..7442)
                     /gene="gatY"
                     /locus_tag="ACHKXP_003631"
     CDS             complement(6588..7442)
                     /gene="gatY"
                     /locus_tag="ACHKXP_003631"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO7648562.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7751..8803)
                     /gene="fbaB"
                     /locus_tag="ACHKXP_003632"
     CDS             complement(7751..8803)
                     /gene="fbaB"
                     /locus_tag="ACHKXP_003632"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO7648563.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9060..10337
                     /gene="yegT"
                     /locus_tag="ACHKXP_003633"
     CDS             9060..10337
                     /gene="yegT"
                     /locus_tag="ACHKXP_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO7648564.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10334..11338
                     /gene="yegU"
                     /locus_tag="ACHKXP_003634"
     CDS             10334..11338
                     /gene="yegU"
                     /locus_tag="ACHKXP_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO7648565.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11335..12300
                     /gene="yegV"
                     /locus_tag="ACHKXP_003635"
     CDS             11335..12300
                     /gene="yegV"
                     /locus_tag="ACHKXP_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO7648566.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12274..13020)
                     /gene="yegW"
                     /locus_tag="ACHKXP_003636"
     CDS             complement(12274..13020)
                     /gene="yegW"
                     /locus_tag="ACHKXP_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7648567.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13072..13890)
                     /gene="yegX"
                     /locus_tag="ACHKXP_003637"
     CDS             complement(13072..13890)
                     /gene="yegX"
                     /locus_tag="ACHKXP_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO7648568.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(13955..14755)
                     /gene="thiD"
                     /locus_tag="ACHKXP_003638"
     CDS             complement(13955..14755)
                     /gene="thiD"
                     /locus_tag="ACHKXP_003638"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO7648569.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14752..15540)
                     /gene="thiM"
                     /locus_tag="ACHKXP_003639"
     CDS             complement(14752..15540)
                     /gene="thiM"
                     /locus_tag="ACHKXP_003639"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO7648570.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRTQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15575..15671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15763..16035)
                     /gene="rcnR"
                     /locus_tag="ACHKXP_003640"
     CDS             complement(15763..16035)
                     /gene="rcnR"
                     /locus_tag="ACHKXP_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO7648571.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16156..16980
                     /gene="rcnA"
                     /locus_tag="ACHKXP_003641"
     CDS             16156..16980
                     /gene="rcnA"
                     /locus_tag="ACHKXP_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO7648572.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17199..17537
                     /gene="rcnB"
                     /locus_tag="ACHKXP_003642"
     CDS             17199..17537
                     /gene="rcnB"
                     /locus_tag="ACHKXP_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO7648573.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17619..18653)
                     /gene="yehA"
                     /locus_tag="ACHKXP_003643"
     CDS             complement(17619..18653)
                     /gene="yehA"
                     /locus_tag="ACHKXP_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO7648574.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNSSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18669..21149)
                     /gene="yehB"
                     /locus_tag="ACHKXP_003644"
     CDS             complement(18669..21149)
                     /gene="yehB"
                     /locus_tag="ACHKXP_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO7648575.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDLAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGAGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQSLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPSSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21165..21839)
                     /gene="yehC"
                     /locus_tag="ACHKXP_003645"
     CDS             complement(21165..21839)
                     /gene="yehC"
                     /locus_tag="ACHKXP_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7648576.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(21919..>22393)
                     /locus_tag="ACHKXP_003646"
     CDS             complement(21919..>22393)
                     /locus_tag="ACHKXP_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7648577.1"
                     /translation="DVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPTTVFEGKAK
                     YSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVGYYLYLGD
                     NSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
BASE COUNT         5648 a         5701 c         5157 g         5887 t
ORIGIN      
        1 gtttagttga atattaggta tattctcttt tttgaaattt atcggtggct gccgttagta
       61 ttcgctgtcc ccattgcaag ctcctggtgg taaccactga atcctccata cttgaactga
      121 cttttatcct ccgactttca tcctgttctg actccacctt ttcttttctg ctctacacta
      181 tctacagacc aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga
      241 ttcttaatgg caaaagtact gacaatttac ccttgcgcga agcaattatg ctgttgcgtg
      301 aggaaggaat gacgatccat gtgcgggtca cctgggagaa aggcgatgcc gcacgatatg
      361 tagaggaggc ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca
      421 ttaatgaagt ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa
      481 ttttgccatt aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg
      541 ataaggcgct gaaactggca attgccggta acgccattgc gatagatatg gcgcaggtca
      601 acaaacaaac ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag
      661 aaacgccgga aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa
      721 tgcgcatgga tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc
      781 aaggtgacgc cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcagcaat
      841 tgtgtccgaa cgcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg
      901 aaatacttcc ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg
      961 aaggcgcttc gtcgaggttt gatattcagg caccacacga catcaccttt aatcttgatg
     1021 gcgaaccgtt gagtgggcaa aattttcata ttgaaatact tccggcagcg ttgcgttgcc
     1081 gtctgccacc agattgtcca ctgttgcgtt aatcaaacta ttctctcttg aaccagggag
     1141 aatccgtaat gccggatgcg gtgtaaacac cttatccggc atacagaaca atactacacc
     1201 gtaattaatt ttacccgcgt cttctgcaat gccagtttat ccacctctga cacagcatca
     1261 tcggtaataa taatgtcaat tttttcgatt ggtagtacct gattaaaacc gcgacggtta
     1321 aatttcgatg aatcgagaac ggcaatcact ttattagcgg ctgtgaccat cgcaccgcta
     1381 atagaataac cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg
     1441 acaaacatta aatcggcatt aatatcctgt aatgaacgct cggcaatgga accatgcatc
     1501 gagcgcgttt tatggcggac cgtgccacca cagacgacca gagtaatgtc tttattttcg
     1561 gaaagggcaa acgctgccgg gagactgttg gtaatcaccg tgatattttt ggcggtcatt
     1621 aatccttcgg cgataagcat tgtcgtactt ccgctgtcga gaataacggt catcccttcg
     1681 tggatcattt tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat
     1741 cgctctttaa acccgacttc ctgggtctcg gtttcactat taccagacat tattttcgcc
     1801 gcaccgccat gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg
     1861 attgtcgctt ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt
     1921 tcattcacta attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca
     1981 tattcaattg tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca
     2041 ataccgggcc agcatcacgc tggcccgcgg tttcagggaa tgagcaacac tttgcccggc
     2101 atagcattac gagcgatgtc acgcaccgcc tgggcgaagc tttcaaagct tccacggtga
     2161 gcgattaatg gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac
     2221 tcctgccccg gccaagggct ggagtagttc atccaactgc cgataaccgt cagctcttta
     2281 cgcaatattt tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc
     2341 agcgccagtt gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg
     2401 ccagccgtct cgaggataag ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc
     2461 ggcgcgctca tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc
     2521 agtttttctg aactaatgtc gatcgccgtc acactctttg ctcccagcgc gacagcgcac
     2581 tgaatggcca gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa
     2641 ccttgcgcta aatgaaaagc atgcagacca acggtaatcg gctcaataaa agccccatcc
     2701 tcaataggca tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca
     2761 gcaaatccac catcacgccg cgagccaata aaatcatatt ttgcgcactg ggaataaaac
     2821 cctttcagac actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg
     2881 ccagggtgta aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg
     2941 cctaacgtta ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca
     3001 cataagcccg agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg
     3061 acgctttctg caacgcgcac gataccatca gtatcattca ccactgattt cataaaaact
     3121 cctgattgtt aagggggata acctccccct caggtaaaaa ttattctgcg agaacgactt
     3181 tctcttgttt aataaagcca cgcgctctac gccaggtcat gaaaataccg gtcagataaa
     3241 ttgcaccgat aataatgaaa ccgggaatat tttgcgggga gaaaacctga atcagtaacc
     3301 aggtaatggg agaaccgccc tgatccattg aagccaccat acccccggct tttaacgccc
     3361 cagcattagc cgccagttgg gtgtgcaaac caatagtttg cgtcgcgatc cacagggtga
     3421 tgctcataat gatgacaccc gagattaagg tgcggaacag atttccacga tgcacggcga
     3481 ccgccatcgc cacgaagaag ccgatggtgg caagatcgcc aaacggcagc acctgattac
     3541 ccggcacaca aacagcaatt aaaatggtga gtgggataaa aatcaggctt gccgatacca
     3601 ccgccgtatg tcccagcagc aacgccggat caaggccaat caggaactcc tgaccgccga
     3661 acttcgcctg taaacgacta cgagcctgct tagcgatggg cgttaaacca tccatgatgg
     3721 gtttaatcac ccgtggcatt agcagcatca ctgccgccgt ttttaccgcc agctgcaata
     3781 cacctttgac atcgtaaccc gcgaggatgc cgataatcag ccccatcaca aaacccacgg
     3841 tgacaggctc gccaaatgga ccaaatttgc gctgaatatc gtcggcgcta aatttaattc
     3901 ggttaacgcc tgggattttc tcgatgatag catcgaccag caccgcaatc ggccccatat
     3961 acgccgacgt accgtgcgga atagcaatac cttccagctc aaagaaattt cgggtatcgc
     4021 gggcaaacca gtcgccgagc ttataaacaa acgccgcgtg aattaccaca cctgccatcc
     4081 ctatcatcca tgaaccggtt gccagatgca gcaacgcgcc ggtgaaggtc atatgccaga
     4141 tattccagat atcaacattt accacccgcg tcatacgggt cagtaacatc gccacgttaa
     4201 ccagaatcgc aatcggaatc gccaccagcg caatttgcga cgcccaggtc attggtgaag
     4261 agcccggcca gccaacatcg accacatgca gattcaggtc gaaattttcc gccatcgctt
     4321 tcgccgccgg gccaatggaa tccagcatta agccaatcac aaggccaatg ccaacaaagc
     4381 caatcccgat atgcagaccc gctttaaagc aatcgcctgc cttcatgcct aatattttag
     4441 aaaaaataat gatgacaatc ggaagcatca ccgtagggct gaggtcgaga atataacgca
     4501 tgacttctga aaacataggt cacccctgta agatagtcag aattttattt tgtaatgctt
     4561 cgataccgac accagaaata aaaggcatgc cgtgaactaa cggaatatcg ccaaaactac
     4621 gatccacttt ggcagtggtg catatcaaat gcacaccatc catataggtt tctatttcat
     4681 taacccgaca ctggattaat tcaacaggaa tattatgatt ctgacacaac tctttaattt
     4741 cttccgccgc catcgtagag gtcgcaaccg cgcctccgca agcgacaata atcttgcgtt
     4801 tcataaatag tcctttttat tgttacagag ggatttattc gtctgaatct aaaacatact
     4861 ttgtgaagta ttcctttaac tgagtttcag gaagagtaat tagtgtctcg acgatatcgg
     4921 gctgttgtaa cttgccaaat aaacagcgta aaagtttcaa ttgctgctgc ggattttcca
     4981 caatcaacgc aataaccaac gacacctcca cgtcgttatc atcatccgct tgctgaaaat
     5041 gaactttatt tgttggcctt aacagataaa tggctgacga cttagcatga atcgcctcac
     5101 aatgcggtat agcaatggcg tgctgctcaa gcattatccc ggtagggaat tctgcttctc
     5161 tggcaattaa tgcctgtggc caggtatcat aaaccacacc tttcgcgagc atctcattac
     5221 cgatatgggt taaaacttcg ctacgatcga caaaagaaat tccgctacga acaaacaggt
     5281 tagtcatata ccgtccttat tccgcacagc cgtagcgata ggcgcgcaaa acatcataaa
     5341 ttttatccat aatcagctga tgcggtattg ctgataattc cccggactga atgcgttcaa
     5401 attgtttggg aagatactga ctaatcatgc ccagtgggat gtccacgcct tcaagattca
     5461 ccatcatcgt ttcgacgcta tttttaatcc gactatgcgg ccagtaataa cgaatacgat
     5521 ccgacaggct gtaacgaata tccagtaatg aatcgttaaa acccgtacga taatattttt
     5581 tccagtattg cggttcgtcg agcatcactt cttcaattac cgccaggcaa ccgctgcgat
     5641 tttcaggggc gataagttcc tgctcaattt gtgccagtgc aaatatcgcc tcgcgtaaag
     5701 caaaggttaa tgcgggaccg actttcaata ttgcaaagtg atcgcggact aattcccaat
     5761 aagccgtccg ggtctggtaa tcggtagaat gtgcttcata aaccattcgg gtgctttcta
     5821 tccattgcgc cagcggctgc gcttcctgcg gctgataatg gataatattg ctgtgatcaa
     5881 attccacgcc cggctgcacc acgatggcaa tcacgcgtgt taacgcctct gtcagcccac
     5941 gggcaataaa ggccttttga tgcgtacgta aagtattggc ggcatcttca acatgggtga
     6001 tgtgtactga ctgaatggcg ctggcctcac cgcccggaac cggaacttcg gtgccaatga
     6061 catagctcag ttgctcacgc tggcaatctg tcgccacact ttccgcagca aagcaaagca
     6121 cagcagctcg ttccgcaacc gtttctggtg ctaacggtat gggatccccc gcgcaggaca
     6181 ttgacgcatc aagatgaatt ttactgaaac cggcacgaac atattccttt accagctcga
     6241 cggatttttc catcgccaca tccgcatttt cttgctgcca gcagtttggc cccagatgat
     6301 cgccgccgag aataatgcgt tcgcgtgcaa acccaacttt atcggcaatc gtaaaaacaa
     6361 attcgcgaaa gtctgccggt gtcattccgg tataaccgcc aaattgattg acctggtttg
     6421 acgttgcttc aatcagcact ttgcgcgtgc tgttgcgatc aaatgccagc gccgcttcga
     6481 taaccaacgg atgggcagaa cagactgaac atatgccgat atgttcacca gctttatgcc
     6541 gggcaattaa cgttttcatg ttttttcctt attaaatggc aggtgcgtta tgccctgccc
     6601 tcgcagccac aatcggcaat cactttgctc accacatcgc gcattgcgga tttagccgac
     6661 tgcaaataat cccggggatc ggtcgcttca gggtgctcgg tcaggtaatt ttttaacgcc
     6721 tgcgagaagg catttttcag ctccgttgca acgttgattt tgcatatccc cagtttgatg
     6781 gtttgctgaa tatccttagt cgataaccct gacgcgccat gcagcaccag cggtaagttc
     6841 acccactggc gaatgttctc cagtcgagaa aaatcaagcg ccggtgcgct ggcatacatc
     6901 ccatgagccg tgccgatggc gaccgccagg gaatcaattc cggttgcctc ggcaaattca
     6961 cgcgcctgag cggggttggt ataaaaagca tcggcttcat tgacttgcac atcatcttcc
     7021 tggccgccaa gttgccccag ctccgcttcg acgctgacat caaagcgatg gcaaaaatcc
     7081 accacctctt tgacccgtga aatattttgc gcaaaaggca aatgcgaggc gtcaatcatg
     7141 actgagcgca cgccagaacg aaccttctga gcgatatcgt caaatttcgt gtgatggtcg
     7201 agatgaattg ccagtggatg gtgatattgc ttcgccatcg cgctgaccag cgccaacaga
     7261 ttttctgtac cagcatgagt aaatgtgcca ggcgttccgg cgatgatgac cggcgcatgc
     7321 aggttggcag cggtttccac caccacttgc atcgtttcga gattgtgaat attgaatgcc
     7381 ggaaccgcat aaccgccgcg ctgtgcgttg ttcagcatct gctttgtcga taccacgtac
     7441 attttcatat cctgtcgttt gctttcgatt tcaaaatata atgaaatgat ttgtttttaa
     7501 atgtcgagat aacgatcaca aaaacgacaa tgtgaaaatt gttcgaggag taaaaggcaa
     7561 aaaaaacggc ctcccgatag ggaagccgta gcaaagtgcg cgtgttttta tgccggatgc
     7621 ggtgtaaaca tacgtggcag tgctatgtag gcatgataag acgcttccgc gtcgcatcag
     7681 gcataggttg ccggatgcag tgtaaacgcc ttatccgtcc tacggggtgg tgctgttcgt
     7741 atgaggcgta tcaggcaata gtaattttgc tatcgagata aacgtcctgc acggcattaa
     7801 tcagtttcac gccgtcagcc atcgatttct tgaacgcctt acgtccaaga atcagcccca
     7861 ttccgccagc gcgtttgttg ataaccgcag tacgcactgc atcgctgagg tcagtttcac
     7921 cgcccgcagc accgccggag tttatcaacc cagcccgacc catatagcag ttagctaact
     7981 gataacgcac cagatcaatc gggttttcgc tggtcaattt gctgtaaaca cgatcgtcgg
     8041 tgtaaccgta attaattgct ttatagccgc cgttattttc cgccattttt tgtttgacga
     8101 tatctgcacc gatggttgcc gccagatggt ttgcctgacc ggtcaggtcg gcggaaacat
     8161 ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa ataggcccac agcactgtca
     8221 ccatacccag ctcgtgcgca cgttcaaaag ccgcagaaat ttcttcaatc tggcgacgtg
     8281 actcttccga gccaaaatag atagtcgcac caaccgcaac cgcgcccatg ttgaacgcct
     8341 gctccacgct ggcatacagc gtttgatcgt aggtattcgg gtaacttagc gtctcgttgt
     8401 gattaagttt gacgaggaat ggaatgcgat gcgcataacg ccgcgatacc gacgccagca
     8461 cgccgtaagt tgacgccaca cagttacagc ccgcttcgat cgccagttca acaatgtttt
     8521 tcgggtcaaa gtagagcggg ttagcagcaa atgaagctcc ggcagagtgc tcaacgccct
     8581 ggtcaaccgg cagaatagaa agatatcctg tgccagccag acgcccggtg ttgtacaacg
     8641 tctgcatatt acgtaacacc gctggcgggc gattattgtc aatcattacg cggtctacgt
     8701 agtcatgtcc ggggagataa agctggtcag aaggaattgt catacaacgg tgctgtaaaa
     8761 ggttgtcggc gtctttgcca agcaactgcg caatatctgt cattactatg ctcccgtaaa
     8821 ttccgattgg atatcggcta tggattgtcc tagcccgcct tttgcgggca atcataatcc
     8881 tggtcgttac gggcgagttt ttccatcttt tggatgtttt ttcagcgttt tctgttggct
     8941 cgattcatca gaaaaaatgt tagcgcggtc aagttttcac cgcaaaggta tttaaaaggt
     9001 attattaagt ggtattgtca tcgcgtacct tacattacct gtcatgaagg aattaaaaga
     9061 tgaaaacaac agcaaagctg tcgttcatga tgtttgttga atggtttatc tggggcgcgt
     9121 ggtttgtgcc attgtggttg tggttaagta aaagcggttt tagtgccgga gaaattggct
     9181 ggtcgtatgc ctgcacagcc attgcggcga tcctgtcgcc aattctggtt ggctccatca
     9241 ctgaccgctt tttctcggcg caaaaagtgc tggcggtatt gatgttcgca ggcgcgctgc
     9301 tgatgtattt cgctgcgcaa cagaccactt ttgccgggtt cttcccgtta ctgctggcct
     9361 actcgctaac ctatatgccg accattgcgc tgactaacag tatcgctttt gccaacgtgc
     9421 cggatgtgga gcgtgatttc ccgcgcattc gtgtgatggg cactatcggc tggattgcct
     9481 ctggtctggc atgtggtttc ttgccgcaaa tgctggggta tgccgatatc tcaccgacta
     9541 acatcccgct gctgattacc gccggaagtt ctgctctgct cggtgtgttt gcgtttttcc
     9601 tgcccgacac gccgccaaaa agcaccggca aaatggacat taaagtcatg ctcggcctgg
     9661 atgcgctgat cctgctgcgc gataaaaact tcctcgtctt tttcttctgt tcattcctgt
     9721 ttgcgatgcc actggcgttc tattacatct ttgccaacgg ttatctgacc gaagttggca
     9781 tgaaaaacgc caccggctgg atgacgctcg gccagttctc tgaaatcttc tttatgctgg
     9841 cattgccgtt tttcactaaa cgctttggta tcaaaaaggt attattgctt ggtctggtca
     9901 ccgctgcgat ccgctatggc ttctttattt acggtagtgc ggatgaatat ttcacttacg
     9961 cgttactgtt cctcggtatt ttgcttcacg gcgtaagtta cgatttttac tacgttaccg
    10021 cttacatcta tgtcgataaa aaagcccccg tgcatatgcg taccgccgcg caggggctga
    10081 tcacgctctg ctgccagggc ttcggcagtt tgctcggcta tcgtcttggc ggtgtgatga
    10141 tggaaaagat gttcgcttat ccggaaccgg taaacgggct gactttcaac tggtccggga
    10201 tgtggacttt cggcgcggtg atgattgcca ttatcgccgt gctgttcatg atttttttcc
    10261 gcgaatccga caacgaaatt acggctatca aggtcgatga tcgcgatatt gcgttgacac
    10321 aaggggaagt taaatgaaaa cagaacgtat tctcggtgct ctttatgggc aggcgttagg
    10381 ggatgcgatg gggatgccct ccgagctttg gccacgcagc cgcgttaaag cacactttgg
    10441 ctggattgac cgttttctgc ctggaccaaa ggagaataac gcggcctgtt attttaaccg
    10501 cgccgaattc accgacgata cctcgatggc gctgtgtctg gcggatgcgt tactggaacg
    10561 tgaaggcaag atcgatccgg atctgattgg gcgtaatatt ctcgactggg cgctgcgttt
    10621 cgacgccttt aacaaaaacg tactaggtcc gacctcgaag attgcgctta acgccattcg
    10681 cgacggtaaa cccgttgctg aactggaaaa caacggcgtg accaacggcg cagcgatgcg
    10741 cgtctcgcca ttaggttgtt tgcttccggc gcgtgatgtt gattccttta ttgatgatgt
    10801 ggcgctggcg tcgagcccga cccataaatc cgatctggca gttgcaggtg cggtagtcat
    10861 cgcatgggcg atttctcgtg ccattgacgg agaaagctgg tcagcgattg ttgattccct
    10921 gccttcaatt gcgcgacatg cacagcaaaa acgcatcact accttcagcg cctcactggc
    10981 agcacgtctg gagattgcgc tgaaaattgt gcgcaatgcc gacggcaccg aatccgccag
    11041 cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg ttccgtgcgc
    11101 cattgcgctg gttgaactgg cacaaaccga cccgaatcgc tgcgccgtcc tgtgcgctaa
    11161 ccttggcggc gacacagaca ccatcggcgc tatggcgacg gcgatttgcg gcgcgttgca
    11221 tggcgttaac gctatcgatc ctgcattaaa ggcggaactg gatgcggtaa atcagcttga
    11281 tttcaaccgc tatgccacag cgctggcgaa atatcgtcaa caacgggagg cggtatgagc
    11341 ggcgctcgat tacacacgct gctgcctgaa ttaacctcgc gtcagccggt gatggtcgtc
    11401 ggcgcggcgg tcattgatgt gatcgccgac gcttatgccc tcccctggcg tgggtgcgat
    11461 atcgaactga agcagcagag cgttaacgtt ggcggctgcg cactgaatat tgccgtggcg
    11521 ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc tcggtcaggg cgtgtgggcg
    11581 gagattattc gcaaccggat ggcaaaagag ggcttaatca gcctgatcga taacgccgaa
    11641 ggtgataacg gctggtgtct ggcactggtt gagccggatg gcgaacgcac ttttatgtca
    11701 ttcagcggtg tggaaaatca gtggaatcgc caatggttgg cacgattaac cgttgcgcct
    11761 ggcaacctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga attgttagtg
    11821 gaatggctgg aaaagctgca agacgtgacg ccgtttatcg attttggccc acgtatcggc
    11881 gatatcccgg acgcattact ggcgcggatc atggcctgtc gacctttagt atcactcaat
    11941 cgtcaagagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat aacaacactt
    12001 ggcaagcaat ggcaggagaa atttgccgcg ccgttgatcg ttcgtctcga taaagaaggc
    12061 gcatggtatt tcagcaacga cgcttctggc tgcattccgg catttccaac gcaagttgtc
    12121 gacaccattg gggcgggcga cagtcatgcc ggtggcgtac ttgccgggct ggcctctggt
    12181 ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgtgggt tgtcgggcat
    12241 cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacaa aaacgtatag
    12301 atcgctgcga cagtggctaa tgctgtactc aataggccgt tgctgttggt caagcgccac
    12361 ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact gaaactcggc
    12421 atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt gggtaaatgt gctggctgcg
    12481 gaaatagtca taaagtgaaa tcccgatggc atcaacatca tgaattaaat gcgccggaac
    12541 ccacgattcc tcaatcgata ccgcttcttc atcgacatag cgaatacgct taagcaaaaa
    12601 gacatcactt cctgcttcga ccgccagctg ctgcgcgact tcttcgggac atttcacgac
    12661 acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct gctgagaaaa
    12721 acctcgcgct tctttcaacg agtattcgaa gatgttgttg atctgcgtgc cgtaaccgcg
    12781 cgaacgcgtc accacaccct cttcttccag cgcctgcatc gccttgcgca ccgtaatgcg
    12841 cgaaacaccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc catgctccag
    12901 caccccgctg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa gcggcgtgtt
    12961 atccgccgct aaaatacgct cgttcaactg agcgataagc tgggtatgcg cttgttccat
    13021 ttgtcttttt cctgccgggg agtctggtgg gagtatatca ctgcttctta tttaaggcgt
    13081 ttctttaatc ccatcaacga atgcctgcaa ctcctcctct gtgccattaa acacattaaa
    13141 atccaccgga ccattaatgc catctacctg cccacggtcg gaatgctgcc agaaacgcca
    13201 ggccatgccg tcattgtccg gacgacgttg atagtagtga gccacccacc acggatattc
    13261 attgaaatag cccgccagat tggtgtgata aaaaacggct cctgagtaaa taatcggctt
    13321 ttttcccgta cttttttcga ccatttttag ccactgactt acccgcttgc gtaattcttt
    13381 tgccgataat tttccccgtt cttctacgtc cagcacggca gggaaatcgc cttgtgagaa
    13441 atccaccgtt tgcagaaata atcgtgcctg aaccggcgct gctaccgacg gggagaaata
    13501 atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac gcgaaaaata
    13561 ggggtccacc agcttttcgc cttccgtcgc cttaataaaa gcaaactgta agcggatacc
    13621 gttgtcgcgc atttttgcca cccgctgcca gtcgatccgc tcctgccagc gagaaacatc
    13681 tattccgtga atggtgtaac tggcaggaat gcgaatagcg aaagatttaa ccgggcgata
    13741 accgtagaaa tggatgtagt ctttaatctg aattgctgtc gaatagaaaa aattcaccgt
    13801 ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata gagaggtgag
    13861 tttttttcga ctggtaattc ttagctgcat tggtttcatc cctgaatgtc agtgcaaaag
    13921 actgacaata accaaagcag ctatagtacg gtgctcacca ccaggcgtgg aagtggtgaa
    13981 ccggaccaat accgtgacca acttccagcg tgtcggcctg ggctaacgcc gatgaaagcc
    14041 agctttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgt agtgcagcca
    14101 acgccgcaga gagtgtacaa ccagtgccgt gggtgttttt ggtcataatg cgcggtgcgg
    14161 taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg gctttgctca tcatccagat
    14221 gaccaccttt cattagcact gcgccacagc ccatcgccaa cagcgatcgc ccttgttcca
    14281 gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcggcagct tcgggcaagt
    14341 ttggcgttat taatgaaacc tgtggcaata atcgactgcg cagcgtagca accgccgaag
    14401 gtgaaagcag cgggtcgccg ctttttgcca gcataacggt gtcgagtacc acgttttgga
    14461 tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcggtttcc gccaacatac
    14521 cgattttagt ggtatcgatt cgcacatcgc tgaacaccga atcgagctgg gcggcgacaa
    14581 aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc accagtgcag
    14641 taataactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg gcctgaatcc
    14701 ccgcaccacc actcggatca gtaccggcaa tcgtcagagc gttaattcgt ttcatgcctg
    14761 cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt ggaacaaaac tgcctggccc
    14821 ctcgcttctg gcgactgcgc gttctccggc ttgtttcatc cagtgacagg cagatgcgac
    14881 attttccagc atatcgcctg gtaacgcaca acaggcagcg acaaccgccg ataatgcaca
    14941 gccagttcct accactttgg tcattaacgg atcgccaccg tgaatgccaa tgatacgatg
    15001 tccatcggta acataatcca cctcgccagt gaccacgacg attgcgccag tttcccgtgc
    15061 cagtgtttgt gcagcgggta tcgcgttagc tgcggcgtca gtggtatcca ctccccgccc
    15121 accattagca atgccagcta atgccatgat ttccgaagca ttaccacgta tcgctgccgg
    15181 tttaaaagat aaaagttcat gacaaaaatg gcggcgataa tcgagcgcac ccaccgctac
    15241 tggatcaagc gtccagggtg tttgagagct ttttgcttgc tcaacggcag cacgcatcgc
    15301 ctgtgtgcgt ggctgcgtca gtgtgccaac gttaatcaac aaggcactgg cgatagccgc
    15361 aaactgactg gcctcttcgg tttcgataac catcgctggc gatgcaccga gcgccagcaa
    15421 ggtattggca gtaaaggttt gcaccacatc gttggtcatg cagtgcacaa gaggggaatg
    15481 ttggtgaaaa aggtgtaacg tgtgcgcaga ttgcgctgaa ctcagcaggt cgacttgcat
    15541 agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac ttccctacgc
    15601 tggcattatc cagatcaggt gatacgggta tttctcagcc ttcacgcaga agggcacccc
    15661 gagtcgtttg gttgcgatga taaatcgcag aggaggatgg taatgtccag cgcacgcgtt
    15721 gtaaacgagg aaaaacggca gttttacaat cgcgttaata aattatttga tatatgaatc
    15781 cagcaccttc agaacgacat ccagatcttc ttcacgtttt agctcatccc cctggtgaac
    15841 gatatgttcc gttagatgac ctttaatcac ttcccgcatc agaccgttta ccgcgccacg
    15901 gatagcagca atctgttgta aaactgcagc gcattcgtgc ggctcgtcga gcattttctt
    15961 gagcgccacg acctggccct gaatcttact ggcacgcgct ttcagtttct gtttatcacg
    16021 gattgtatga gacatggcaa cacctggtta acaagaacat gaaaaatcat agcactatta
    16081 atctactggg gggtagtatc aggtactggg ggggagtaga atcagattgc cgaattaata
    16141 ctaagaatta ttatcatgac cgaatttaca actcttcttc agcaaggaaa cgcctggttc
    16201 ttcatcccca gcgccatctt acttggtgcg cttcatggcc tggaaccagg gcactcaaaa
    16261 acgatgatgg cggcgtttat catcgccatc aaaggcacca ttaaacaggc ggtgatgctc
    16321 ggactggcag caaccatttc gcataccgcg gtggtctggt tgattgcctt tggcgggatg
    16381 gtgatcagta agcgctttac tgctcaatca gcagaaccgt ggctccagct gatttccgca
    16441 gtgatcatta ttggcaccgc gttctggatg ttctggcgta cctggcgcgg cgaacgcaac
    16501 tggctggaga atatgcacgg gcatgattat gagcatcatc accacgatca cgaagatcac
    16561 cacgaccatg gacatcatca ccatcacgaa catggcgagt atcaggatgc ccatgcacga
    16621 gcccatgcca atgacattaa acgacgcttt gatggtagag aggtcaccaa ctggcaaatt
    16681 ttgctatttg gcttaaccgg tggccttatc ccctgcccgg cagcaattac cgtgctgttg
    16741 atatgcattc agttgaaagc cctgacactg ggcgcaacac tggtcgtcag tttcagcatt
    16801 ggcctggcgt taacgcttgt caccgtaggc gttggcgcag caatcagcgt tcagcaggtc
    16861 gcaaaacgct ggagcggatt taacactctc gctaaacgcg ccccctattt ttccagtctg
    16921 ttgattggct tagttggtgt gtatatgggc gtacatggct tcatgggcat aatgcgataa
    16981 ctcaatcctt taggcttgca tgttatcttc agcccaggat gattcttaaa tcagctattt
    17041 ccgctgactc tctgctcgct aaggatgctt ttagggcatc ctttcattta cactttttac
    17101 gaaatcatgg gatcactaac aaaatatcgc ttgtcagtta tattgtatgg caggaaagat
    17161 atgcgactga tattacagat ccccaaagtg gagagtttat gaccattaaa aataagatgt
    17221 tgctgggtgc gcttttgctg gttaccagtg ccgcctgggc cgcaccagcc accgcgggtt
    17281 cgaccaatac ctcgggaatt tctaagtatg agttaagcag tttcattgct gactttaagc
    17341 atttcaaacc aggggacacc gtaccagaaa tgtaccgtac cgatgagtac aacattaagc
    17401 agtggcagtt gcgtaacctg cccgcacctg acgccggaac ccactggacc tatatgggtg
    17461 gcgcgtacgt gttgatcagc gacaccgacg gaaaaatcat taaagcctac gacggtgaga
    17521 ttttttatca tcgctaaaaa aagccccctc ggagagaggg ggaaatgcag acaccttatt
    17581 atttcttatt attagccact tgctcgtctt gcttgttatt agtcgtattt cacgttgatt
    17641 aatgcggttg cttccagtgc gccagattta actttgtttg tatcgtagac gtagtaactg
    17701 gctgttatcg gaattgaaac cgttgtatct gtaagttttg actttgccgc aatttctttt
    17761 aaatttgtgt atcctttact gctgttagtg atatttaaaa gtgtgactgg cgaactatta
    17821 ctttcaagaa taaatcctac gccattatct tccgcacctt tcagtattgt gttgctgttg
    17881 gttaacaagt cagaacttga taaatacacc tgcattttcc gtgatgtttg attttgttga
    17941 cctaataaat ttttacaacg cagaataata tccccttgtt tactatttat tgttgctttg
    18001 ttacctgtgg ctggtaattg tgaaacggga atactgtcta aagtaattaa taaatcgtcc
    18061 ggtttgcagg ttgtggttat cgggttgtat gacaatgtca gattaaagga atagacacct
    18121 gcattttgtg ccaatgcatt tctgtagcta tttacacatg catcccatga cttgccacct
    18181 aataaacaca gtagaaattg tactgcggca ctcccgcgtg tcgatgatga agcactggat
    18241 aatgttgcaa caccattaat catcactgaa ccgccgcccc ccgcagaaac attcactttt
    18301 gcattactgg ccgcaggtgt caccgttacc gaataaccat tggtgaaact ggtctgatta
    18361 tttgacaacg tgatgttatt tttattggtc cattcaaaat gtaaatttaa tttaatggta
    18421 tcgttataaa gtccaacaat aatatccgac gggctcatag gggtgatagt atttgtactt
    18481 acgcatgacg aatcattaaa attatgctca tatgtcgcca tttgtgtttg agtaagtgca
    18541 aaatccccct gaacaaccaa gttgtgctga acagataact cactataaca tgccgcatag
    18601 ctcacaggca taaccatcat cattaatata aatagcatta tgcgaatttc catagaacca
    18661 cctgactatt actggcaaat ataatttcta ctttcatcaa tctctttacc gaaggtgatt
    18721 gtgcatgaaa gtccttgttg cttatcaatt gccacattaa ccgatgacgg tacttcattg
    18781 gtgcgaataa ataactgact tccctggccg acaacgccaa tattatgacc atggatatca
    18841 ttgacttcat aaccaaacgt taatgattgc ccatctgctc ttaacgcttt tataaaccat
    18901 ggcttgcgct gatcggtatc aaaattaacc agtacaaccg cgccgcgata aggggcggca
    18961 attttccggt tgccacgtaa ttctgcttcg ctatcgcttt gcgacacatc cagcatcagg
    19021 tgattttccc gataaggtgt cattccgtcg tatatcacca ctccattacg gtttgttgtg
    19081 cgatattttt gcccattgac ataagcatct ttaattcctg gcgcattcat cacagcaaac
    19141 gtttcggaaa gacggttcgc cagattaacg ccacccgacc aggcgacaat gccccctgaa
    19201 acactggctc cagcctgtcg ataagtactc gactgactat aactgccatt cactgtcgca
    19261 accggcgcgt tccaggtcaa attcgcccca gctgtcgttt catttccctg atgctgataa
    19321 ctcaggttga ccccatagtt aaactgatcc cggcttccaa cggttcctga taatcccgta
    19381 ttatttgagg caaacccctg atcatcaaac gtcgttgagt tagacatata tatttgccga
    19441 cgaggcgtcg aaacgtcatc accccaatca aagggaatcg atataaaaat attaaaacgt
    19501 ttctcttcat gatgattctc gtcataagcc tggcttgccg cgagggtata gcttatccgt
    19561 cgcaggttgt tggaataact caactgataa tccttactac tgccgctacg cccccagtaa
    19621 tctcgccata acgtacttaa tgacacagac ccccaacctt ctggcaatga ctggctcata
    19681 ttggcggaaa agctattttt gcggccaaaa tcgttctggt aataatcggc aatgtcatag
    19741 acatcgtttt catcacggcg ataattatct ttattgtttg cccaaacgtg atcgttaaat
    19801 gtccggtaat cacgcgacga ataacgccag gccgccagac caaaacgcgt cgacgtttgg
    19861 ctcacaaatt tgttgtaggc aatttgataa ctttgcccgt caaacacatc gccgttgtct
    19921 tgtttactat gcgacttagt ggcatcgacg gaaatggcac caatgcgtgt attccagcca
    19981 gcccccaaag taaacgcgta ataattattc gcgaccatcg agccaccata cagcgtcaat
    20041 aaattattaa aaccatactg ataacccgcc tggacaaaat cactttgttt gctcgcccct
    20101 tcaatatggc tacgacccgc cgctaaatca tatttcgaca cgccgggttg cagcatattt
    20161 ggcaccgctg cataaggcac cagataggtg gttaccgagc cgtccgcctc tttcacgctg
    20221 acatcaagat ctgctccacc accggccaac tgcaaatctg taatcgcgaa cgggccagga
    20281 ggaacctctt tctgataaac cacaaaacca ttctgttcaa tagttaccag cgcgttactc
    20341 tgagcaatcc cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa catctgcata
    20401 tcacgaaaca accgcacacc tctgaagcga acagaatcaa aaatatcgct tgatgtgtac
    20461 atatcaccca cgcgaagcgt gccgagaagt tgggcaaatc cacgttccag atacagggta
    20521 ttgcttttcc acacccctgg attgttattt gttttactga aactggcatc agaatgcagt
    20581 tgccacccca gtaaatttaa cccgctgtta aaacgtacat atgtactctt gttattaccc
    20641 gacgctttat agtcgctgta atactgactc agataataag aggtataaaa cgcattaata
    20701 ccccgctccc agttttccgg tggaacatag ccactttcca gttcttccac ccaggcctgc
    20761 gggacactga aatcgagacg aaaaaccccg atatcccagg tatagctccc accctgaaca
    20821 agttgctcaa atgttaaaca ttgcttaccg ctggcgaagt tatcgctatt aatgcctaac
    20881 cgcttgataa cttctcttga taaacatgtt tcttgcgggt tgtctttaac aataatctca
    20941 tatttcccgc gccattgctt attgacataa atatcgatgt catactgccc cggtaagggt
    21001 tgattatcat caagacgaat atttgcaacc tgctggtctt tcattccacc tatcataaaa
    21061 tgggtatcaa aggtttcttc agctgcataa gcttcaatgc cgagcaataa cgctacgatt
    21121 gctgatgcaa gtggggtcat tctcaacata ggcagctcct gcaattaaat tttgtcacta
    21181 atatagttgc catggtcatc gataatggtc agatgccagt tatttgcatt attacttttg
    21241 acattaacac tctgactttc taagggcgca atcataatag tttcataatt gactttgaca
    21301 ttattagctt tgacatccga aatcgtcacc caattagctg agtcattttt tatcaccaaa
    21361 ccattgccac tgcgatttac cagcaatttt ttaaatgtcg ctttatttac cggagcaata
    21421 cccgctggcc ggtaaaacaa cttaattctg ttttgcatcg caaacttcag tgcattctta
    21481 ccttcttgct ctggactatt tggtggaata tccagaacat tcagataaaa aatgctttct
    21541 ttattagtgg gcagtttatt cggcataatt ttgattttta cttgctgccc ggaatttgct
    21601 cctatttttg ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaat
    21661 gacgtatcgc catcatcaat ccacgcctgc agcagcgaag aacggtttcc ctggttcatc
    21721 aactgcacca tcacttcttt attttctgcc gggtaaataa tgcgcgtccc gtagataaca
    21781 attccggcat gtgcaggaag gaccatagaa aaaatgagta aagataataa tcctttcatt
    21841 ttaatgcctc ttaaattaaa aggccgccat gggatagcgg ccatgtttat tacctggaga
    21901 aacttacaat tttatttatt aatcctgcgc cacacgcata accagagaag ataacacatc
    21961 acctgattgc actgagttat ctgtcagttt caggtatttt gctttaaacg gaatagcata
    22021 ttgaccttcc gctgttttat aactctcaat gttaaaaggt gcagaaacat ccaggacatc
    22081 gctgttatca cctaaataga gatagtaacc cacggtatcg ttgctactgg ctatcgcctt
    22141 atcgttaccg gttattttat cgttggtaat caacgagagt tttacaccag caacttcttt
    22201 cgggcagcta atttttaaca tgctgctggt tacaccgact gcatcatcat aggtagaata
    22261 tttagcttta ccttcaaaga cagtggttgg tacagtcggc atattaatac ttactgaatc
    22321 accacccgct tcgaatttac acggagattc tgcaatattc cccttaatag ttaatgttcc
    22381 ggtatcaacg tct
//
LOCUS       DAZFZC010000084        22145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFZC010000084 DAZFZC010000000
VERSION     DAZFZC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.084"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            181..831
                     /locus_tag="ACHKXP_003647"
     CDS             181..831
                     /locus_tag="ACHKXP_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648578.1"
                     /translation="MANIIISKKSIIEAASIVSDELRDKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKSGKCVYSIADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGAGSAKAGTQQYRFIKNDFYNYLADAFKA"
     gene            880..1083
                     /locus_tag="ACHKXP_003648"
     CDS             880..1083
                     /locus_tag="ACHKXP_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648579.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELIDDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLQNSI"
     gene            1104..1334
                     /locus_tag="ACHKXP_003649"
     CDS             1104..1334
                     /locus_tag="ACHKXP_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648580.1"
                     /translation="MFVLIAGVNVHNEYYVNRIAGIAGYAGRAVELIDETTRKIDLLS
                     DQERKKADVNDADIFLMLKAFVEMGFEISLHK"
     gene            complement(1951..2433)
                     /locus_tag="ACHKXP_003650"
     CDS             complement(1951..2433)
                     /locus_tag="ACHKXP_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648581.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(2784..3158)
                     /locus_tag="ACHKXP_003651"
     CDS             complement(2784..3158)
                     /locus_tag="ACHKXP_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648582.1"
                     /translation="MGLDIYIETQPKNDLNNEASRKQVAYFRKFNALVGWMERNVGEV
                     ENCELLELTMNDICLLKAHLMHINESNCEEYLPTREGFFFGSQEYDEGYWHDVDKLKK
                     LVEDLIRNHDFHNNRLTFCAWW"
     gene            complement(3276..3671)
                     /locus_tag="ACHKXP_003652"
     CDS             complement(3276..3671)
                     /locus_tag="ACHKXP_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648583.1"
                     /translation="MTVKREKLTVDVYYASETAEGKNVAKITVVTYNTETGAEVQAST
                     IVRKGDASGGEYATQYQSILDATDPLLLKIENYFRQVDEEVFETMMNMVNTVFASSLN
                     TSTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            complement(3798..4109)
                     /locus_tag="ACHKXP_003653"
     CDS             complement(3798..4109)
                     /locus_tag="ACHKXP_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648584.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            complement(4263..4592)
                     /locus_tag="ACHKXP_003654"
     CDS             complement(4263..4592)
                     /locus_tag="ACHKXP_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648585.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     EFLEVFA"
     gene            complement(6163..7353)
                     /locus_tag="ACHKXP_003655"
     CDS             complement(6163..7353)
                     /locus_tag="ACHKXP_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648586.1"
                     /translation="MTDTAFSKSLQKEVDPEQYLALKNLDDSSVHAIARDDIICPICK
                     VGGGSFVRATRNGGYHKKAHFRFTGEDGQGHHPSCDFYGDRLTSEVKQHLVSFTKDRT
                     KYSQVIRKLVCAGIQEGIFTQEKMWQMREWFFNKRKDSTFEIWLESEHLDWLYYISGL
                     RDAYLAWTTPDILPFAPIQVTVPGFSWKRAIDKEVLRVHIKTLQQLREISVSHRDIAV
                     ILEHINNNRDRTILDPSHLEDEISKTCKLTSFVMGNYIEFQTKTVSDRAYGEAKFLAF
                     AALLLFVSDWDLNIAIGKFSKIARVKEVDDMLAGNFIGLNPYFRYDLANAVKRLQDNW
                     PIEYQELEHWNVEKSMRDAYEKWRATEPEFAPPLLPDLYIAKHEKEVAQDEQVRQWIK
                     NGDV"
     gene            complement(7524..7745)
                     /locus_tag="ACHKXP_003656"
     CDS             complement(7524..7745)
                     /locus_tag="ACHKXP_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648587.1"
                     /translation="MIASNELDWVSQIDGGCIVHAGLQDDVWKDDLRRYGISEGAIAN
                     IQKVLDVGFDSVHFDCGAPIVEGLECWCW"
     gene            complement(7985..10018)
                     /gene="metG"
                     /locus_tag="ACHKXP_003657"
     CDS             complement(7985..10018)
                     /gene="metG"
                     /locus_tag="ACHKXP_003657"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO7648588.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            10176..11276
                     /gene="apbC"
                     /locus_tag="ACHKXP_003658"
     CDS             10176..11276
                     /gene="apbC"
                     /locus_tag="ACHKXP_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO7648589.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            complement(11314..11703)
                     /locus_tag="ACHKXP_003659"
     CDS             complement(11314..11703)
                     /locus_tag="ACHKXP_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7648590.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKYPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(12500..13171)
                     /locus_tag="ACHKXP_003660"
     CDS             complement(12500..13171)
                     /locus_tag="ACHKXP_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648591.1"
                     /translation="MMHKSVAGEFQKEVDNTTDLLDDILSILALLEAGDWSEHCTKTE
                     LGGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSDRVE
                     KVDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWVECTRAAY
                     VRAKDKGELTRVVAHHPNNELKDHQIRELVNELRDIAVQYHGTQQLREKIARAVNNSI
                     RSESM"
     gene            complement(13168..13389)
                     /locus_tag="ACHKXP_003661"
     CDS             complement(13168..13389)
                     /locus_tag="ACHKXP_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648592.1"
                     /translation="MNNAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRGYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            complement(13408..13656)
                     /locus_tag="ACHKXP_003662"
                     /pseudo
     CDS             complement(13408..13656)
                     /locus_tag="ACHKXP_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(13821..14006)
                     /locus_tag="ACHKXP_003663"
     CDS             complement(13821..14006)
                     /locus_tag="ACHKXP_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648593.1"
                     /translation="MRTLEVRAEDVIPGDVLITSKGQQCAVKSFWMENDKVTLFGTDG
                     SETDYDYDELLVVERAA"
     gene            complement(14003..14533)
                     /locus_tag="ACHKXP_003664"
     CDS             complement(14003..14533)
                     /locus_tag="ACHKXP_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648594.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDSGKVMIFPAQPLTTLTPEQVADITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIFTKEGAAR"
     gene            complement(14530..14853)
                     /locus_tag="ACHKXP_003665"
     CDS             complement(14530..14853)
                     /locus_tag="ACHKXP_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFO7648595.1"
                     /translation="MTTSHSAITQEKAFNILERLETLATEEDISPEKLVEFSRVILRR
                     KNDMERLTSGAPSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGE
                     KEGSA"
     gene            complement(14904..15251)
                     /locus_tag="ACHKXP_003666"
                     /pseudo
     CDS             complement(14904..15251)
                     /locus_tag="ACHKXP_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(15248..15748)
                     /locus_tag="ACHKXP_003667"
     CDS             complement(15248..15748)
                     /locus_tag="ACHKXP_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648596.1"
                     /translation="MREKIKNPVVVLYKRETSDSYAVSITDGSQNMHDGLLMASVSPD
                     DSDYPFATFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDAMCAEEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDMARNAMIDFVDGEVGPNKNVPGLIRGAEICVSIAEQL
                     RKGVIQ"
     gene            complement(15748..16290)
                     /locus_tag="ACHKXP_003668"
     CDS             complement(15748..16290)
                     /locus_tag="ACHKXP_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648597.1"
                     /translation="MKVEKIDVLSFVLTDLERLDPVRVMIENYEPGKGRITIICYGKA
                     WTAAWFAMGGDDVQTFIKRVSNEYLIGYFDSQLRSTVDDDNDANLLFVKSEIIKLRRE
                     REIDAVLAREMWDEAENADDVKESCCCFGVGNKLLNLFGDDPWYADWPTVPNPKYQYL
                     ERIVNAVREGLKELDKLEAS"
     gene            complement(16287..16928)
                     /locus_tag="ACHKXP_003669"
     CDS             complement(16287..16928)
                     /locus_tag="ACHKXP_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO7648598.1"
                     /translation="MSSISEAHMFAAGAHGGVGQKRKYTGEDYINHPVAVREIVALHD
                     GTVEMQIAALLHDVVEDTHVTIEMVRDHFGERVAEMVQALTNIARPEDGNRIQRFIIN
                     VRELEQNLDMQTRMIKLADLLDNTSSIVSRDPEFSAVYLAEKELMLDVLFHGKEIGAN
                     ADVVEYMERIGIEHPLLLSAMAKVTEGIALLKPVHIKRYEKHKSLIWSALEAA"
     gene            complement(17048..17428)
                     /locus_tag="ACHKXP_003670"
     CDS             complement(17048..17428)
                     /locus_tag="ACHKXP_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648599.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMPNQPILDGHIQMVINSHEKGWAAIVPCTITYKLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILSGN"
     gene            complement(17428..18132)
                     /locus_tag="ACHKXP_003671"
     CDS             complement(17428..18132)
                     /locus_tag="ACHKXP_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648600.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GNFDESAKTFIDFVAKRWSEQWKDLEKRASEFDRFMDAMDTAKEALAAGTPLDLESLF
                     NGEVASAMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQRKEGKTPGERVAELE
                     AVVDQRNGECDRLVNELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            complement(18191..19879)
                     /locus_tag="ACHKXP_003672"
     CDS             complement(18191..19879)
                     /locus_tag="ACHKXP_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7648601.1"
                     /translation="MNTALSIIDDASSNTAIDYRQEMNVIHEIVAECEKEIAFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRGWLDKVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWREQFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISMPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKPGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTMAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVKR"
     gene            complement(20021..20587)
                     /locus_tag="ACHKXP_003673"
     CDS             complement(20021..20587)
                     /locus_tag="ACHKXP_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648602.1"
                     /translation="MCEKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERLF
                     TILNFNSTFEEPTQIVALAHHFGVHYLAEKERVDKLQATLDMVSETQGTHDGNKSETI
                     IASKDREIAGLKSSLMMLMSAINLMSSKAGYKMPSLNSDDPMAVRQLLGAMADQLDDT
                     KSRLEDMMRELTHRHDLNKQPHKTQYAL"
     gene            complement(20727..20885)
                     /locus_tag="ACHKXP_003674"
     CDS             complement(20727..20885)
                     /locus_tag="ACHKXP_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7648603.1"
                     /translation="MLERFRLVIVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKAKC"
     gene            complement(20885..21310)
                     /locus_tag="ACHKXP_003675"
     CDS             complement(20885..21310)
                     /locus_tag="ACHKXP_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HFO7648604.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGAYMISYADGTCDA
                     KEIAILEKTIAALPAFSPFAGEIAQMSANIRARYEASPRSANAQAIRELSDIAGTPEA
                     VDVLCLCLDIADQDGIGEQEEQALKKIAQALQLSLDAYL"
     gene            complement(21404..21592)
                     /locus_tag="ACHKXP_003676"
     CDS             complement(21404..21592)
                     /locus_tag="ACHKXP_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648605.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            complement(21602..22096)
                     /locus_tag="ACHKXP_003677"
     CDS             complement(21602..22096)
                     /locus_tag="ACHKXP_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648606.1"
                     /translation="MARQTYFTSATKRPRSLRQILAELFSGRVMSRLDELETTVRLLN
                     ERLDNQASVVANVGAIVASGSSREAKNARPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRSVPFDAGFIHHHTFVDDNYHHSSGASCHSGWDGGGCDTSSSSSYSG
                     SCCD"
BASE COUNT         5691 a         5322 c         4870 g         6262 t
ORIGIN      
        1 aatcgattat ttatttaggg aacaccttcg acgatctcgc ttttatttct aggactttca
       61 tccctgcaaa aaatttaaaa aaaacacttg caatcttttt tcgcgtatcg ataattgaac
      121 tcgtcgaaag cgaagacgct aacgacaata aattttaaat ttacataagg aaaaattatc
      181 atggctaaca ttatcatttc taaaaagtcc atcattgaag ctgcatctat tgtatccgac
      241 gagctgcgcg ataaagcaga tctggcaact caaacatata acgaacatta taaaaatggt
      301 acgcacacta aagcagacaa agcaaatatg caagctgcga ccactaaact tgcttacttc
      361 atcaacaacg tcgtaaacgc agtagaagac gaaaaattat gctctgtttt ctactatgcg
      421 attaaagcaa gcaaacaagc gccagaagta tttttccgtg atgcaatgac taatagttat
      481 tctctggaaa aactggttta tctggttaaa tcaattaaat ctggtaaatg cgtttattcc
      541 atcgctgata tgtcaggatc tcgtgtattc gctttaatcg atatgattaa cgacgagatc
      601 gacacgttca ccaatggcgc tgttttcgat ttaatgaatg aagctaaaaa agcgtgtgaa
      661 attaaattgg acgctggcta tactcaagcc aaccagttga tcaatctttg cgaacgtctt
      721 ggacttgttg aaaaagtcaa aggagctggg agtgcgaaag ctggtactca gcaatatcgc
      781 ttcattaaaa atgatttcta caattattta gctgatgctt tcaaagcgta attaatggaa
      841 tcaagcgccc actatgggcg ctttaaagga gctttgaaaa tggtcagcta tgacaagatc
      901 cgcgcggaat atcgcgcaaa gtatcgcgct tataaactgg aattaatcga tgatttaatc
      961 gcgcaacgcg accaattaaa ttttacgttt tctgatttgc ttaacagcaa gcgagattgc
     1021 aagcgcaaac gtgaatactt acgtttaagc gcattaatcg gaaaacttca aaattctatt
     1081 taattttttt aaggagctta atcatgtttg ttcttatcgc tggcgttaac gtccataacg
     1141 aatattatgt taatcgcatc gctgggatcg ctggttacgc tgggcgcgca gtcgagctta
     1201 tagatgaaac gacgcgcaaa attgacttat tgagcgacca ggagcgaaaa aaagcagacg
     1261 tgaacgacgc tgatatattt ttaatgttaa aagcgtttgt agaaatggga tttgaaatca
     1321 gtctacataa ataaaatcga gcgcccacta tgggcgcttt tttcgtttcc aatacttcca
     1381 ccataacgcg ccaatgatgg cgcttttttt attgtctttg actcaagcca acaacataaa
     1441 aataagcgcc aaaataacgc catagacgcg cttctatatc cttaccagta catacccatt
     1501 acttagcgca ttaacgcgct taaatcgcgt tatattgcgt tatggtgtat ggttaatcat
     1561 tggcttttat tcttgctatg tgatccgcgt ttaattgtcg gcgcggatcg gcattttgtt
     1621 ttgttccgta tccgctcgcg tattatgtgc gcgtgatttt tcacataatc acaccactta
     1681 atcacatatg tgattacgct acgaaagaaa tctggatgtc tcaggcgacg aaagtcatca
     1741 tgattttccc cgctcacctg tccgacaacc gctggttgga ttccaccagc ttcccgatgt
     1801 tttttctaca caaaggcgaa tgcagccgtt tcccgaaaaa atcctggccg ttccccgtcg
     1861 gctcacgaat acgttcctcg ccgtttctga aaatttccct gcggcagctg gtggctatag
     1921 agaaagggcc gtttctggcc ctcttctcag ttacacgcca tcaaggatgt ggatgcggtt
     1981 gcttgcgtat acattcagca taaagttagc gcaaaacagt ttccatgtat caacgccagc
     2041 ggtatacgta atgtttttgc atttaattgc attgttggcg atccgcatcc cctgcgatac
     2101 tgcttcatcg tcggaaaatt tgaacgacga ttgagtttta atccagacag aaatctgcgt
     2161 agcgaactca atcagcttgg actggcagaa tcgcccggag cgcaccggaa agacgaacgt
     2221 tccgaatcca gaatttacca catacgcttt ctcaaatacc cgcgagtaac gacgattgcc
     2281 aatgatgtcg cgtgcaataa tgcatttttc ttgtgctgac agttctaccg tctcattgtc
     2341 gcgccatgca ccaagtactc gtttttcaat gtcagagaac gttacagcga tattgccatg
     2401 tgcgggtgcg tttacagtag cgataaaatt catgattaat tccttatcgt aaataacaaa
     2461 ttgttttctt gtttgtgtta attatcgttg tacgaataag gcgtcaaagt ggaaagttgc
     2521 ggtagccgga cgggaacagg tgggtttgtc ggttgcctgg aggtaagagg ttggtgtttt
     2581 tagcctgcgg gaaacaggat ggtcatttaa ggccaccaat gctgatggcc ttaataatta
     2641 tcgaccgatt acgctaagga tcttttcctc aactgatttg ttgttgcgac taaactgcct
     2701 tgcgtatctg atgacagaag aggcgttttg ctttctgacc atctcggctc tttccttcag
     2761 gcgtttcttg aaatcgccca tatttaccac caggcgcaga aggtcagtct gttattgtga
     2821 aagtcgtggt tcctgatcag gtcttccaca agttttttca gcttatccac gtcatgccag
     2881 taaccttcgt cgtactcctg actgccgaag aaaaaacctt cccgagtagg caagtactct
     2941 tcgcaattgc tttcgtttat gtgcatcaaa tgagctttca gaagacaaat gtcattcatc
     3001 gttaattcta aaagctcaca attttcgact tcacctacgt tgcgctccat ccacccaacg
     3061 agcgcattga acttacggaa gtaagcaacc tgctttctgg atgcctcgtt attcagatcg
     3121 tttttaggct gtgtctcgat atagatatca agtcccatga tggtttcctt attaagttgc
     3181 ttcagtgaaa tcattttcac aaatcagata aggcagaaaa caaattgtta tcgggcataa
     3241 taaaatggcg cggtttgcgc gccatttaaa gaggattaag cgaatacgct ttcagggatt
     3301 aaggtgtcgg caggaatgcc agaggtaatg cgcagaccgt actggccaat ccaggtggtg
     3361 ctggtgttca ggctggaggc gaatacggtg ttaaccatat tcatcatggt ttcaaacacc
     3421 tcttcatcaa cctggcggaa gtagttctcg attttcagca gcagcgggtc agttgcatcg
     3481 agaatggact ggtattgagt cgcgtactcg ccgccggagg catcaccttt acgcacgatc
     3541 gtactggcct ggacttcggc accagtttcg gtgttgtacg taacaacggt gatttttgcc
     3601 acattcttac cttcggcggt ttcagaggca tagtaaacat caactgtcag tttttcgcgt
     3661 ttaacggtca tttcactctc cgtagtgtct tggttatttt tctattctat gatcgtaagt
     3721 atgtacttac aatacaaaaa agccccgtaa ggatggcggg gctgtcgtac tgtcgactaa
     3781 ttaccacttg cacattatca ggctattgcc atcactgatg ctctctgaat ttcttgctgg
     3841 gccacgcggt taacttccat caaagccagt tcgagctgat gttccggcca cattctttga
     3901 aatgccatcc agccttttcc acgctggcga cgaacattca ttacataacg agtaaaggtg
     3961 tttttatcga aagacaccgc tgtttcacgg aacagacgaa tagaggttcc attggcaacg
     4021 atgtctagca aagttaattg agcaaccagt gcaggcttct cttttctctt ctggtctttg
     4081 tccagaccaa gaaccaatga ttttttcatc acaactccgt aaacaacttg ttttctaaat
     4141 ggcgtaaata ataccagtaa gaaaacggct acaaagcata atgatcaggt gcatgatcga
     4201 tatgcaggtt cctcccagcg gctatagaga aattaagcca gcaatgctgg ctttggttgg
     4261 gattacgcga atacctcaag aaactcctga acgtcccaat gctggacgag gtgaagatag
     4321 accttagaaa tcacgtcatt ttcgctatcg catgtgaagt agtacttctg gctgcggttg
     4381 tatgtgtcca cacaaaattc gctcatatcc ataaagtcag catcttcacc catgtcgcaa
     4441 atcatcaaag caggtgcgtc atcacgagtc atcagctcaa gcacccaccg cgaattgatc
     4501 agagtggaag accaaccttc taactcataa aactgatcaa actctttttg agttaatttc
     4561 ttgatttggt tgatgtcgat atttgcaaac ataaagcaat ccttaaacaa catgttttct
     4621 tgatggtgtt attatcgcaa taacgcacag gcgaaaaagg attttattcc ggtgaatgtg
     4681 gttttggcag tgagttttca ggttcagtac cgagtacact ttttacatta ttctgattta
     4741 gtggccgaaa gtggctggtg ggcagaataa tcctctcccc gaaaacctac tgaaatcgat
     4801 tgtgacccgt tggctgtctg gcacaattac gctgtagctg ggcattcaga gggaatccag
     4861 cggtaagcaa gtccgcctac gtgagcgcct ctcttcttcc cgcacattac aggtgggtag
     4921 ccgcaactct ccctctcgct ataccggtac gccaccgttc ctgaaccggc atcgagcctt
     4981 ctcccgaaat ccacacaggc aactcgaccg tttccctgaa aacctccagc cgttccccga
     5041 aggcaaccca gccgtttctg tagcgggatg cagccttttc cctgtatgga ccgaaggctg
     5101 accgtagggg tgttacgggg gataaaatca ggagttgaat aaatacgaaa aaggaggcga
     5161 cacccctcct tccctctcca cttatacata cctggcttct tcgctataca tgttgtgttt
     5221 tcttcgtgtt ttatctgtct caacctctct ataggcgtta atgctcactc cttccagaac
     5281 gttctatacg gcgactattt ctgttggtga attgggtgtg ttgttttctc tgtcggtgta
     5341 tgtggtttcg ttagttttgc ggtggttctt ctatggggtg ttctcgtcgt tttggtgatt
     5401 gttcttctcc gtgtatggag taatggcagg tgggcgaatc ctttcgttat tgtgtttgag
     5461 ttgtttgtct ggagtgtgaa gggcttttgt agttattcgt gtattgggta atggcgtttc
     5521 ccgtgattgt tctgtttcgt gtcttttggg attgagagaa tccttgcggt aacaggcttc
     5581 gttggggttt cagtggttca ggtgtttctt cgctgtttgg gtaatggcgt gtcgtgggta
     5641 tcgcgcgtat gaatattttc ggattgcctg tgaaagttag gttggttgtc cggtagcctg
     5701 gggagaagag gtgggtcttt tcggtagcct gccataaaga ggttggttgt tttggggaat
     5761 ggcatgtgtg ttatttagtt gttttgttat agctgaaaat aagttgttta tatagttatg
     5821 taacgcaatg ggagtgattt tgagcgtgtg tttttgttgg tagtgtgttg ggtcgtctgt
     5881 ggtgaaaatt ggacgctgtg gcgctgtctg ctgcgtagaa ttacgggtat aagaagtgat
     5941 gtgtgaatac gtcaattttt tagaccaaat cgggtgaaag cgttgacgtt tcgttgatct
     6001 gttatttagt tgttttctta ttggtataaa caacgttaac ctattgatat ttaaaggtgt
     6061 gattgcaggt atggaagaag agtagtggcg atcaacggat tcttatagaa aagaatcaaa
     6121 ttgtgaccgc cattatcaac tgattgatta agctaattac actcaaacat ccccgttctt
     6181 aatccactgc ctgacttgct cgtcttgtgc aacttctttc tcatgtttgg caatatacaa
     6241 gtctggcaat aggggtggag cgaattcagg ctctgtcgca cgccactttt catatgcgtc
     6301 acgcatggat ttctcaacat tccaatgctc caactcttgg tattcaatcg gccagttatc
     6361 ctgcaatctc ttcactgcat tagccaggtc gtatcggaag taaggattaa gaccaatgaa
     6421 attaccagcc agcatgtcat caacctcctt cactctggca atcttagaaa acttaccaat
     6481 agcaatgttg aggtcccaat ctgacacgaa caacaaaagt gctgcaaatg ccagaaactt
     6541 agcttccccg tatgctctgt cgctaaccgt ttttgtctga aactcaatgt aattgcccat
     6601 cacaaaacta gtaagcttac aggtcttact tatctcgtcc tccaaatgcg atggatcgag
     6661 aattgtgcgg tcacgattat tgtttatgtg ttctaagatt acagcaatgt ctctatgtga
     6721 gactgaaatt tcacgcaatt gctggagagt cttgatgtga actcttagca cctctttgtc
     6781 aatcgcacgc ttccaagaaa aaccaggcac agtaacctga atgggcgcga aaggtagaat
     6841 atcaggagtt gtccatgcta gatatgcatc acgtaaccca ctaatataat aaagccagtc
     6901 taaatgctcg ctttctaacc atatttcaaa tgtggaatcc tttcgcttat tgaagaacca
     6961 ttccctcatc tgccacattt tttcttgagt aaatatccct tcctgtatgc cggcacagac
     7021 cagctttcgt attacctgcg aatactttgt tcgatctttt gtgaatgaga caaggtgctg
     7081 cttcacctca gatgttagtc tatctccata gaaatcacat gatggatgat ggccttgacc
     7141 atcttcaccg gtaaatctga aatgcgcctt cttgtgatag ccaccattcc tagtggctct
     7201 aacgaacgaa ccacctccaa ctttgcaaat ggggcagatg atatcgtcac gggcaatagc
     7261 atgaaccgag ctgtcatcta aattctttag cgccaagtac tgctcaggat cgacttcttt
     7321 ttgtaacgat tttgaaaaag cggtatcagt cattccaagc tccgagcaag gggttatttg
     7381 ttcaattatc attcggaatt atttactgtc tcgcccagtt tcacgtcatt ttattcccta
     7441 gtctagcttt tctagggtat acagcttatt acaagctcct cattattaac tgtttccgta
     7501 actcattgat ccgcttatac actctaccaa caccagcact caagcccttc aacgatcggc
     7561 gcaccacaat caaaatgtac agagtcaaag cctacatcaa gtactttctg aatgttagca
     7621 atcgcgcctt cggatatacc atatcgacgc aagtcgtctt tccagacatc atcctgtaac
     7681 ccggcgtgga caatacaacc gccatcaatt tgtgaaaccc aatccagctc attgcttgcg
     7741 atcatgaatc ggcgagcatc ttcctctgat gtatgagatg aactgatcaa tgccacctgg
     7801 taggcttggg taatgttcaa catttcaacc actccgaaac aatttgtttt cttatgtggc
     7861 ttattatctc aatcaacaaa aggtagaaaa caaattgttt tagggcttgt gtaaatcagg
     7921 agggattgtg tctatatgaa aatgcccagc gtgttgccgg gcattgcgaa cagaggttgc
     7981 ttaattactt aacctggtgg ccaggtttag caccagcatc cgggcttaac aggaagatat
     8041 ctttcccgcc ggggccagca gccatcacca taccttcgga gatgccgaag cgcatcttac
     8101 gcggagccag gttagccacc ataatggtgt gacgaccaat cagtgcctgt gggtctggat
     8161 aagcagaacg gatgcctgag aagacattgc gtttttcacc gccgagatcc agcgtcaggc
     8221 gcagcagttt gtcagaacct tcaacaaact ctgcgttttc gatcagtgcc acgcgcaggt
     8281 caactttggc gaagtcgtca aaggtgatgg tttcctgaat cggatcatct gccagtgggc
     8341 cagttactgg cgcggcagcg gcttttactt cctctttgga tgcttccacc agtgcttcaa
     8401 cctgcttcat gtcgatgcgg ttatacagtg ccttgaacgg attcacttta tggcccagca
     8461 gcggttgctg gataccatcc caggtcagtt ctgtattcag gaatgcttct gcacgttcag
     8521 tcagtttcgg cagtaccggt ttcaggtacg tcatcagcac gcggaacagg ttaatgccca
     8581 tagagcaaat cgcctgcaga tcggcatcgc gcccttcctg cttcgccacc acccacggag
     8641 cctgttcatc gacatagcgg ttagccagat cagccagcgc catgatttca cgaatcgctt
     8701 taccaaattc gcggctttcc catgcttcac caatcacttc agcagcatca gtaaaggttt
     8761 tgtacagttg cgggtcagcc agttcgcttg ccagcacgcc gtcaaaacgc ttgttgataa
     8821 agcctgcatt acgggaggcc aggttaacca ctttgttcac gatatcggca ttcacgcgct
     8881 gaacgaaatc ctccaggttg agatcgatat catcaatgcg cgaagagagt ttcgcagtgt
     8941 agtagtaacg caggctgtca gcgtcaaaat gattcagcca ggtgctggct ttaataaagg
     9001 tgccgcgaga cttggacatc ttcgcaccgt tcaccgtcac atagccatga acaaacaggt
     9061 tggtcggctt gcggaaattg ctgccttcca gcatggcagg ccagaacagg ctgtggaagt
     9121 aaacgatatc tttaccgatg aagtggtaca gctcggcggt ggagtctttc ttccagtatt
     9181 catcgaagct ggtagtgtcg ccgcgcttgt cgcacagatt cttgaaggaa cccatgtagc
     9241 caatcggtgc gtccagccag acgtagaaat atttacccgg cgcgttcgga atttcaaaac
     9301 cgaagtaagg tgcgtcgcgg gagatatccc actgttgcag accagattca aaccactcct
     9361 gcattttgtt tgccacctgc tcctgcaacg caccgctgcg ggtccatgcc tgcaacattt
     9421 cgctgaaaga gggcagatca aagaagaagt gttcagaatc acgcattacc ggggtagcac
     9481 cagaaaccac cgatttcggc tcgatcagtt cagtcgggct gtaggtcgcg ccgcaaactt
     9541 cacagttatc gccgtattga tctggcgctt tacatttcgg gcaggtgcct ttcacaaaac
     9601 gatccggcag gaacatgcct ttttccgggt cgtacaactg agagatagtg cgatttttaa
     9661 taaaaccgtt ctctttcagg cgagtataga taagctcaga caactgacgg ttctcttcgc
     9721 tgtgcgtcga gtgatagttg tcatagctga tgttaaagcc tgcgaaatca gtctggtgtt
     9781 cctgactcat ttcgccaatc atctgctccg gtgtgatacc aagttgctgt gctttcagca
     9841 tgatcggcgt accgtgggca tcgtcggcac agatgaaatt aacctcgtgg ccgcgcattc
     9901 gctggtaacg aacccagaca tcagcctgga tgtgctccag catatggccg aggtggattg
     9961 agccgttagc gtacggcagc gcgcacgtca ccagaatttt cttcgcgact tgggtcatag
    10021 taggcattac ttctttgtag tgaaaagggg cttgatagta acaaaatggc cttatgtctg
    10081 ccatgtgata acagcatttc tcataaatga ttaaatgtcg cagctggagt acactacaag
    10141 gcgacaattg cgcaaattaa aataaaggag acgggatgag cgagtccaaa tcgccggatg
    10201 ccctgagagc aatggtagcc ggtacgctgg ctaattttca gcacccaacc ctgaaacaca
    10261 acctgactac gcttaaagcg ttacaccatg ttgcgttgat ggatgacaca ctgcatgtcg
    10321 aactaatcat gcctttcgtg tggaataagc cttttgaaga cctgaaagag caatgtagtg
    10381 gtgatctgct ccgtatcact ggcgcaaagg ctattgactg gaagctgtcg tacaacattg
    10441 ccacgcttaa gcgcgtcaaa aaccaaccag gcattaatgg tgttaagaat attatcgccg
    10501 taagctcagg caagggcggt gtaggcaaat cgtccacagc agtaaacttg gcactggctt
    10561 tggctgctga aggtgcgaaa gttggtattt tggatgccga tatctatggt ccatcaattc
    10621 caaccatgct gggcgcggaa aaccaacgtc caacctcacc tgacggtact cacatggcac
    10681 ctatcatgtc tcatggcctg gcaaccaact ctattggtta tctggtcacc gacgacaatg
    10741 caatggtgtg gcgtggaccg atggccagca aggcgctgat gcagatgttg caggaaactc
    10801 tatggcctga tctggactat ctcgttctcg atatgccgcc gggcactggt gatattcagt
    10861 tgacgctggc gcagaacatt cctgtaactg gtgcggttgt ggtaactacg ccgcaagaca
    10921 tcgcgctgat cgatgcgaag aaaggcattg tgatgttcga aaaagtcgaa gtgccggtac
    10981 taggtatcgt cgaaaacatg agtgtgcata tttgcagtaa ctgcggtcat cacgagccaa
    11041 tttttggcac cggtggcgca cagaaactgg ccgagaaata caacacgcag ttgttaggcc
    11101 aaatgccact tcatatctct cttcgtgaag atctggataa cggaacacca accgttatta
    11161 gtcgtccaga tagcgaattt acggctattt atcgtgacct cgcagatcgc gttgctgccc
    11221 aaatgtattg gcaaggtgaa gtaatacctg gcgaaatcgc attccgcgca gtgtgataaa
    11281 aaggtggtgc aatgcaccac ctaaacagtt ctcctacatc acaggacaat catcaaactc
    11341 gccacttctt gcgtcgttga taatgtgagt gatcacacca aacacggcat tgctgcccgt
    11401 gcatccatca tcatcctttg gcaatgcttc cttcttacca gttcttaaat cctccagatg
    11461 ttgacgtgga tacttccggt atctttttac gcgatattcc ccatccagag cacatacaag
    11521 aagagatcca tcaacaggag tcagtgagga atcgaccacc agcaatgcac cctgcaatat
    11581 tccctcacga tgatgactat cagccgccct catgaagtag gtcgctgaag gatgtctaat
    11641 tatctgctga tcaagagaaa tacggctctc agcataatcc gccgcaggag aagggaagcc
    11701 cataatactc gcctcttaat actgtatatg catacagtat atgttgaagc tatatatttt
    11761 tgaactctat ctcttaaaaa agatgttaat aagatgctaa ctattgaaag gcataaatat
    11821 atacaattcc aacaaacgtt atttttaaca aatttttact tcccattgac tttcccagac
    11881 tccttgtctg aactaatgtc cgcggaaacc actttttcct ttacgagtta tccacaaagt
    11941 tatgcacttg cacatagtct ctataaatcc tatcttaatt cgcaagacac tgatgttgcg
    12001 gtattgtggg tacatttggt taaaaacgga cattaaaaaa gcccagttga ctaggctggg
    12061 ctttttaaaa ggaaacagag tttgcgcctc tgttcctgag ccataagtat ctggcgactg
    12121 agctaagatt aaccgaatgt actggattca tcaagaaacg ttgagccgtc atgctaggta
    12181 ataatgacca tttaccaaac atcgttgttc tcaaagaaga tttaagtcca ggtttttggt
    12241 gactctaaaa ccttacctta actcgaaatc gtgccgtaag tatctggcga ctacagatat
    12301 tgcaactgca aaggcaagct caaaggtatt tgcagctagg tgcgataaaa ctaagtgagg
    12361 cctatagtgc ttaacttact tttcaaaatc gtgccgccta tgttcgcaat catcgaagcg
    12421 atcattgagt acgtcaacca gcgcccataa tgctagttgt taccggcccc atccaaatgg
    12481 ggccatttta ttgtattgac tacatacttt ctgatctaat tgagttgttc actgctctgg
    12541 caattttctc ccgcaattgc tgcgttccgt ggtactgaac cgcaatatcc cgcaactcat
    12601 tcaccagttc tctaatctgg tgatctttaa gctcattatt tgggtgatgg gcaacaactc
    12661 tggttaattc acccttgtct tttgctctta cgtatgcagc tcttgtgcat tcgacccatg
    12721 tattaccgcc tacattaact tcgtagatta ggctggactt atctgtcaat tcggaacaaa
    12781 tacgggccgt ggtctggcaa ctagagcatt cgcagttatc gcggaaaccg tgatctactg
    12841 gcttcggttt cacatcatca actttttcta ccctatcgga atttaaatac ttcgaagcct
    12901 tgctatgcca atcgctggtt ttaggatctt gtccatccat caaacgccag gcttcagcga
    12961 ttaactcata actagtgact gtagcttcct gcgcatcacc aataagtcgt gtaatctctc
    13021 tttcaagccg accgcctaac tccgttttag tgcaatgttc tgaccaatcg ccagcctcaa
    13081 gcagcgcgag aatgcttaaa atatcgtcca atagatcagt ggtattatcg acttccttct
    13141 gaaactcacc ggctactgat ttatgcatca ttcgtcatcc tcatccgagt catcctcatc
    13201 acaggatgag agtagtgggt tagtcattcg ccctacttga gtggcgtagc cacgccgaca
    13261 gagattgcgc agcacactgt atatttcgaa catctcggtt cgttcatcgc caacatcaag
    13321 ctcagatgct atagcgtggc attcagtcgc gagagccgat attttctgaa ataactctgc
    13381 attattcact gttcgactcc tgcggcgttc tggtagttgc atccagtgtg tgacattgct
    13441 aatcagacca tattcattag tttgaggatg gttgccgtta tcgtctccgt atttaagact
    13501 ctccataaag ccataatgcc tatcaccatt aacgctcaca aagccgtaat aagcaggtat
    13561 aacgccgatc tcacacgtaa ccagtaaagg aaaactagtt ctccaattta gctcgccaat
    13621 tacaggcatt cgctcactac agcttatcca accatcctta gatgcggctg tattggcata
    13681 ggccttacgg tgcttctgta gctctgctgc aatcgctctc acgacttcaa ctggtgccct
    13741 tgcagcaaac tcaatgttgg tgatcagctc attaagatat ttctcgcttg ggtagcattt
    13801 cttattgtta acggtggtca ttaggcagct ctctcaacaa caagcaattc gtcgtagtca
    13861 taatcagttt cggaaccatc cgtaccgaac agagtcactt tgtcattttc catccaaaaa
    13921 gatttaaccg cacattgttg acctttagat gttatgagca catcaccagg gattacgtct
    13981 tcagcgcgaa cctctagtgt tctcatcgtg ctgccccctc tttggtgaaa atgcctgctg
    14041 caatgctgtt tatgatgctg tcagtgcatg gagtagaaag ctgggcatct ccagcaattt
    14101 tcatgacctc aacatccgca tatcgaatac cgaggtgtat cagaccggct atacctgact
    14161 taagtcgaac attttccata aacagatctt tttcccgctg tttagcggct tccaactcca
    14221 tcgacaattt ttccaactgc tctttatgct tcttgtattc ctgatacgcg tgccaagact
    14281 gacctttgcg cacactatca gtgatatcag caacctgctc tggtgttagc gtggtcagtg
    14341 gctgtgctgg aaaaatcatc actttcccgg aatcccaatc aaaaccagcc tgaattgact
    14401 gaacctcaac tgatggtgtt gaaccgatgc gaccaggcga atgaacaacg atcgttacat
    14461 ccatatcgcg acgatggctg tggttgttgg acaaaatacg attcaccaac tcagaaaatt
    14521 tggaaaattt catgctgatc cctctttctc tccccggata attctgttgc acacatccac
    14581 acactcgtcg cagatgaaaa cggcgtcccc agcaattaac tttttgacgg cgtactggga
    14641 tttgttgcag aagctgcaac aaagtgttcg cctgactgat aaggatggag cgccagatgt
    14701 cagccgctcc atatcgttct tgcgacgcaa tatcacacgg ctgaactcaa ccagtttctc
    14761 cggggatata tcctcctccg tagcgagcgt ctctagtcgt tcgagtatgt tgaaggcttt
    14821 ttcttgggta atagcagagt gcgatgtggt cattttcttt caccttcaat gttcgtggcg
    14881 ctggcaagat tgtccttgcg aagaagagcg gctatgcgct tacttccatc cgagataacg
    14941 ctctcgtaat gctcacgctg ctcgttgagt tttgattttg ccaactccag ttgttttgtt
    15001 agttccgcaa tacggcaaac atcgttgata cgcgtttcct ctaatgcgtt gatctcatcc
    15061 agtagtgcca gcacggtttc tggtccggcc agaaatttga aggcgttgag ggcatcaata
    15121 tcaacaccgc tatctttaag ttcctgttcg cttatcagat catcatcaac tggcaacatt
    15181 aacaggcgtt ccattgccgg aattgcacgc tctgccgcct cacgcagtgc ctgatagtca
    15241 atcttgctca ctggatgact cctttacgaa gctgttcagc aatacttacg catatctctg
    15301 cgcctctaat cagccccgga acgttcttgt ttggcccaac ttcaccatca acaaaatcaa
    15361 tcatcgcgtt acgagccata tccacgccct gcgcacgtac ttcagccaga aaagcatcgg
    15421 tggctggagt ctccgttaat tcgctaaccc aattatctcc gtgttcctcg gcacacatcg
    15481 catcgaatgc tcgctcagat tctttcaggg ctgcattctc cgccgctaat gcgtctctct
    15541 gcgctcgcaa cttctcaatt tcggcagcca tgtagtaacc aaccatagcg aaagtggcga
    15601 aagggtagtc agaatcatca ggagatacgg aggccattag cagaccatcg tgcatgttct
    15661 ggcttccatc agtgatggat accgcataag aatcgctggt ttcccgttta tacaatacga
    15721 cgaccgggtt cttgattttc tctctcatta cgatgcctct aacttatcta actctttcag
    15781 cccttcacgt accgcgttca caatgcgttc cagatactgg tatttcgggt ttggtaccgt
    15841 tggccagtcg gcataccacg gatcatcacc aaagagattc agcagtttgt taccgacgcc
    15901 gaaacaacag cagctttctt ttacgtcatc ggcgttttcc gcctcgtccc acatttcacg
    15961 agccagtacg gcgtcgattt ctctctctct tcgtaacttt atgatttctg acttcacgaa
    16021 aagcagattt gcatcgttgt catcgtcgac cgtgcttcgc agttgagagt cgaaatagcc
    16081 gattagatac tcgttgctga cccgcttaat gaacgtctgc acatcatcac cgcccatagc
    16141 aaaccaagcc gcagtccacg cttttccgta gcagatgatg gtgattcttc ccttaccagg
    16201 ttcgtagttt tcaatcatca ctcgaaccgg atcgaggcgt tccagatccg tcagcacaaa
    16261 ggaaagaaca tctatttttt cgaccttcat gcagcctcca acgcgctcca gatcagcgat
    16321 ttgtgttttt cataacgttt gatgtgtacc ggtttcagaa gcgcaatacc ttcagtgact
    16381 ttcgccattg cgcttaatag caacgggtgt tctataccta tcctctccat gtactcgaca
    16441 acatcagcgt tggcaccaat ctctttgccg tgaaagagta cgtccagcat cagttctttc
    16501 tctgcgaggt agacagctga aaactcggga tcgcgactca cgatagaaga ggtattgtcg
    16561 agcaggtcag ccagcttgat catgcgcgtc tgcatgtcga gattttgctc cagctcccgg
    16621 acattgatga tgaaacgctg tattcgattg ccgtcttctg gccttgcaat gttggtcaga
    16681 gcctgaacca tttcagctac gcgctcaccg aagtgatcac gaaccatctc tatggttaca
    16741 tgggtgtctt cgaccacatc atgcagaagc gccgcgattt gcatctccac ggttccgtca
    16801 tgcaatgcaa caatctctcg gacagcaacc gggtggttga tgtagtcttc gccggtatat
    16861 tttcttttct gaccaacgcc accatgagcg ccagcggcga acatatgagc ttcggaaata
    16921 ctagacattc ttaactccgt tgttttctag ttgtggttat tttcacaaat aagaaaatgt
    16981 agaaaacaat ttatttaagg ccacaactta tggcctttat ttattcatgc cgaaggattt
    17041 gtagcgtcta attgccggat aaaatggggt gaggtgcttg ccagtaggct ccaccggtcc
    17101 atgtgaaccc catgcgaata agagtgttta ttgcggcttt ggtagactcc gattcgacac
    17161 tggcttttcg aaactcttga acctctttcg ccaacttgta tgtaattgtg caggggacga
    17221 ttgcagccca gcctttctca tgactgttaa tgaccatctg gatgtgacca tccagaatag
    17281 gttgattagg catgttattg aaaatgtctg cctgtttttg aatgtgaaga aaaagccttg
    17341 ccagctccat ttgctcacta cgagacaagg gattatcaag agggcttttt gcaaaagcgg
    17401 ctatacgctg tgcgtcaatg ccgaacatca gcgcacctca ccatctttat ggctggagtt
    17461 aacttccgcc attaaatgtc tgacgtaatc gacaagagag ccacctggtg gaatctggca
    17521 ttcctcaact aactggaagt agatatccgc tgcattacgt gtattgctac ccgcacatat
    17581 tctttcttcc cgaagtgcat gaagctcgtt gaccaaacgg tcacactctc cgttacgctg
    17641 atccacaacg gcctcaagct ctgcgacgcg ttcacctggc gtcttacctt ctttgcgttg
    17701 gatggtaacg acgaaatcgc caattgcagg gacgttgtaa tccagctcaa ggtagttttt
    17761 ggcaccgctg cggacgaact cacccgcgaa catggtggcg aacatcgcag aggccacttc
    17821 gccgttgaaa agtgactcca gatctaacgg agtaccggca gcaagagcct cttttgctgt
    17881 atccattgca tccatgaacc gatcaaactc actagcgcgc ttttccaggt ctttccattg
    17941 ctcgctccaa cgtttggcaa cgaagtctat gaatgttttc gcggattcat caaaattccc
    18001 ctcaaactta acgatgcctt tatcaataat gatctggcct accgcatcct ctgcataacc
    18061 tttcaatgta aacttgaaag gaaaatcaga atggcctgtg acgttaaaat gctctaactg
    18121 gaggttgttc atgtgttttt ctcttatcgt ttagtaacta cattcttaca caaattaagt
    18181 aagtatttac ctatctttta acgcgtttga aaacgtatac gctgactgtg atccctgtgt
    18241 cttcaaactc gttggtaaac gacttccctc tggcataaac gtaattatcc atcgtcatcc
    18301 agtccagtat tggtgcagtg cccggcaata ctgctacaag acgcccgccg actttcagat
    18361 gccccagcgc agccatcgtg tgctctctat gacgaccaag cgagtacggc gggttcataa
    18421 cgattttgtc gaactgataa cctgcgttgt cctcagacca ctttatgaag tcgcagcaaa
    18481 tcgtgttcgt ataccctttt ccacgcagga tatcagcgaa gagaggtgcg atttctatac
    18541 aggtaacatc ttccagatcg gcgttaatat aggccagaag atccccacgt ccggcttgag
    18601 gctccagcag cttctcacca ggcttcaatt cagtggctct ggcaacgtac tcagcaatca
    18661 agcgtggggt agggtagaac tggtgtgatt ttgtatccgg tattaatccg gtagccacaa
    18721 tcgtatttag cgtatggccg atctcatacg ggaactgcca gtgctttttc tcctgcacgc
    18781 cgccaatgaa gctcagtgtg cgctcaagct cttccacctg cgacttctgg agagctgaat
    18841 cagagaagta ccatacgcct ttgtctttgc tcaatcgccc gtcacgaagc gcagtgcgaa
    18901 ccggcatgga gatcgtcttc tggattaacc cgaactgctt tggtgcccgt gtttttggcg
    18961 cagttcggca tggcgcgggg attgcagcag gcatactgta agccagcacc tcattcaact
    19021 tccaggccac gtcaggatgt atttcaaagt gaacgttgcc gttcttgaac atcttcacgc
    19081 gcatcagatt tccatcgacg ttcatccagt caccggtctg gcagtcgttt gcccgatacg
    19141 cagctgatag cacctcagca gtgcggttga tggtaataaa ttctttgtgc gcgaagaaat
    19201 gaagcaagac acgcagatcg tcgatgtagt cctctttgcg atagttcacg ctaacgctgt
    19261 cccgccagaa atcagaaatg cagtcagcga ttatcagacg ctcgctgaaa ccgtttgtct
    19321 tatttgtctt gtgcgcagga ctcagcgcct taaacaagcc atacacgcgc tcagagagat
    19381 atttgtgcct gtcattcagc aaattaagca tcgtgggtat gaccgtttct gctttgaact
    19441 ccggtacacc aacgaactct ttaaccttca tctggtagcc ggttctgtca gttttgatga
    19501 cttcctgttt gccctctata aactgctcgc gccactcatc tcgacgggaa gctggcatga
    19561 tcagcaaaac gttagtcatg tccgtgacct tcctccagta ctcggcccaa atattctgtt
    19621 tcacccattc cagatcgact ttatccaacc aacctcgatt taaacgcgag cgatcttcat
    19681 ctggtcgatg gtttagtctc agcaggcgat taatcatgtt gtggcgttcg tcgccataaa
    19741 cgaagtcgtg tacctgatac atgaaggcga tctctttctc gcactcggcc acgatttcgt
    19801 ggatgacatt catttcctga cgatagtcga tagccgtgtt tgagctggca tcgtcgatga
    19861 tggaaagggc tgtattcata attacaccat taaataattt gttttctgat tggtgtaatt
    19921 atcgcaaact tgaaaaggca taaaagctgt attcataggc gttacagcag attaaaggcg
    19981 taaaaatggg ggctttcgcc ccctgtcgac tggtgcagta ttagagtgcg tattgcgttt
    20041 tgtgcggctg cttattcaag tcatggcgat gcgtgagctc ccgcatcatg tcttcaaggc
    20101 gactctttgt gtcgtcgagc tggtcggcca ttgcacctaa aagctggcga acggccatcg
    20161 gatcgtcgct gtttagtgat ggcattttat aacctgcttt agaagacata aggttaattg
    20221 cagacatcaa catcattaga gaggatttaa gcccggcaat ttcacgatct ttgctggcaa
    20281 taatcgtctc acttttgtta ccgtcatgag ttccttgagt ctcgctcacc atatccaaag
    20341 tcgcttgcaa cttatcaact ctctcttttt cagcaaggta atgaacaccg aaatggtgtg
    20401 ccaaagctac aatctgagtt ggctcttcaa aggtggaatt aaaattaagt attgtaaaca
    20461 gtctctcaaa tatggaaacg tcttctattc ctcgaagaat ggctaacatt ttgacaagtt
    20521 cattagcatc catttcttcg aatagggcgt tttgttcatc agctattgct ttgcatttct
    20581 cgcacatgtg ttttccttaa tgcttaaaca agttgttttc ttattggctt tattgtgatg
    20641 tggcatatga ggggagcaag ctaaacgtaa aggtgcaaac tctctatttc ggtcaacgaa
    20701 acacaagggc caaatggccc ttgtcattaa cactttgctt tacgaactaa cggataagcg
    20761 aagtagatcg ccaaaccaat gaggacgcca tcagaaatga cagacatcat ctttcccgta
    20821 aaatccacca acaccgccat caccagaaga gcaatgacga tcacaagccg gaatctctca
    20881 agcattagag ataagcatcc agtgacaact gaagcgcctg ggcgatcttt ttcagtgcct
    20941 gctcttcttg ttcaccaatg ccatcctgat cggcaatatc aaggcacaga cacagaacat
    21001 cgaccgcctc tggagtaccc gcaatatcag acagctcacg aatagcctgt gcattagcag
    21061 aacgagggga cgcttcgtaa cgagcacgga tattggcgct catttgggca atttcaccag
    21121 caaacggaga aaacgcaggc agagctgcaa ttgtcttctc aagaatggca atttcttttg
    21181 cgtcacatgt gccgtcggca taggagatca tgtacgcgcc ccacacggtg gcctcaaccg
    21241 catcacggtt ctccattttt ttgacttcga taactgcttt acgagttttc tttttgaaga
    21301 aacctaacat gtgttttcct tttgttattt gtcaaaacaa gttgttttct agttaaataa
    21361 attgcttacg caaagacggt caatgcatat gcactaacaa ccgtcagaaa taaccaagta
    21421 ccgaaccgac tggaaaaata aaaatcccaa ctacacgagc caaggtcata ccagcctgaa
    21481 actggagatc gccagagcaa acaagtttta taatgttcga tacccaaccg gcggccatga
    21541 gggcaagaat tgccagccaa atttttccaa agtgatttga aaaccagttc atacaaccgc
    21601 cttagtcaca gcatgatcct gagtaactgg atgaacttga ggtatcgcat ccaccgccat
    21661 cccatccaga gtgacaggac gctccactgg agtggtggta attgtcgtcg acgaaggtgt
    21721 ggtgatggat gaatccggca tcaaaaggca ctgaccggct ggaacggcta ccgtcgccag
    21781 tgaaactata atgagaacgt aggccattgg tttcagcctc tttctttgaa aattttccat
    21841 tcgaactgtc cttgttgttt ttctccttca ctaaaggccg tgcatttttc gcttcgcgtg
    21901 aagaaccaga ggcaacaatc gcccccacgt tcgcaacaac tgacgcttga ttatctaaac
    21961 gttcattcag caaccgaacg gtagtctcta gttcatcaag acgtgacata acacgaccgc
    22021 taaataattc ggccaaaatt tgacgtaaag aacgaggacg tttagttgca gaagtgaaat
    22081 aggtttgacg tgccatgtgg accccatcca gtgtcagaaa gagttgcggc tagcgattgc
    22141 cagcc
//
LOCUS       DAZFZC010000085        22079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFZC010000085 DAZFZC010000000
VERSION     DAZFZC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.085"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(12..1430)
                     /gene="yahG"
                     /locus_tag="ACHKXP_003678"
     CDS             complement(12..1430)
                     /gene="yahG"
                     /locus_tag="ACHKXP_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7648607.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(1430..2977)
                     /gene="fdrA"
                     /locus_tag="ACHKXP_003679"
     CDS             complement(1430..2977)
                     /gene="fdrA"
                     /locus_tag="ACHKXP_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7648608.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIGSAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLDRGETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIETI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(2967..3830)
                     /gene="yahE"
                     /locus_tag="ACHKXP_003680"
     CDS             complement(2967..3830)
                     /gene="yahE"
                     /locus_tag="ACHKXP_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7648609.1"
                     /translation="MWALTADAYFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHFHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYLGLQRGKNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(3870..4475)
                     /gene="yahD"
                     /locus_tag="ACHKXP_003681"
     CDS             complement(3870..4475)
                     /gene="yahD"
                     /locus_tag="ACHKXP_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO7648610.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            4733..5230
                     /gene="yahC"
                     /locus_tag="ACHKXP_003682"
     CDS             4733..5230
                     /gene="yahC"
                     /locus_tag="ACHKXP_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7648611.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGVISEYGGQKLSEATT
                     KRDGY"
     gene            5322..6254
                     /gene="yahB"
                     /locus_tag="ACHKXP_003683"
     CDS             5322..6254
                     /gene="yahB"
                     /locus_tag="ACHKXP_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7648612.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(6296..7384)
                     /gene="pdeL"
                     /locus_tag="ACHKXP_003684"
     CDS             complement(6296..7384)
                     /gene="pdeL"
                     /locus_tag="ACHKXP_003684"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFO7648613.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PMAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VNKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(7879..7953)
                     /locus_tag="ACHKXP_003685"
     CDS             complement(7879..7953)
                     /locus_tag="ACHKXP_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648614.1"
                     /translation="MCDILLNVLNIVFIGIAIILVIIC"
     gene            complement(8259..10292)
                     /gene="betT"
                     /locus_tag="ACHKXP_003686"
     CDS             complement(8259..10292)
                     /gene="betT"
                     /locus_tag="ACHKXP_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO7648615.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            10421..11008
                     /gene="betI"
                     /locus_tag="ACHKXP_003687"
     CDS             10421..11008
                     /gene="betI"
                     /locus_tag="ACHKXP_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO7648616.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLYALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKTRANSLTRHFITQHLPTD"
     gene            11022..12494
                     /gene="betB"
                     /locus_tag="ACHKXP_003688"
     CDS             11022..12494
                     /gene="betB"
                     /locus_tag="ACHKXP_003688"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO7648617.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            12508..14178
                     /gene="betA"
                     /locus_tag="ACHKXP_003689"
     CDS             12508..14178
                     /gene="betA"
                     /locus_tag="ACHKXP_003689"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO7648618.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEAQPRSTAGYFVANGMPVRAKK"
     gene            14391..15059
                     /gene="ykgH"
                     /locus_tag="ACHKXP_003690"
     CDS             14391..15059
                     /gene="ykgH"
                     /locus_tag="ACHKXP_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO7648619.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(15302..15997)
                     /gene="ykgG"
                     /locus_tag="ACHKXP_003691"
     CDS             complement(15302..15997)
                     /gene="ykgG"
                     /locus_tag="ACHKXP_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO7648620.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(15990..17417)
                     /gene="ykgF"
                     /locus_tag="ACHKXP_003692"
     CDS             complement(15990..17417)
                     /gene="ykgF"
                     /locus_tag="ACHKXP_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO7648621.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(17428..18147)
                     /gene="ykgE"
                     /locus_tag="ACHKXP_003693"
     CDS             complement(17428..18147)
                     /gene="ykgE"
                     /locus_tag="ACHKXP_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HFO7648622.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(18674..19528)
                     /gene="rclR"
                     /locus_tag="ACHKXP_003694"
     CDS             complement(18674..19528)
                     /gene="rclR"
                     /locus_tag="ACHKXP_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO7648623.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            19754..21079
                     /gene="rclA"
                     /locus_tag="ACHKXP_003695"
     CDS             19754..21079
                     /gene="rclA"
                     /locus_tag="ACHKXP_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO7648624.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRMLGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            21188..21424
                     /gene="rclB"
                     /locus_tag="ACHKXP_003696"
     CDS             21188..21424
                     /gene="rclB"
                     /locus_tag="ACHKXP_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO7648625.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            21436..22029
                     /gene="rclC"
                     /locus_tag="ACHKXP_003697"
     CDS             21436..22029
                     /gene="rclC"
                     /locus_tag="ACHKXP_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO7648626.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
BASE COUNT         5630 a         5684 c         5301 g         5464 t
ORIGIN      
        1 gaacatgtag gtcagacgcc gtaacgttcg caccagccaa gaatggcttt ctcgaagcag
       61 gcaaaaggtg gatgcacaat gcccgcgcca atcatgccga cgcccgcatc tttatgggcg
      121 atggcggtgt tgatcaccgg cagaatgccg ctgctaccca cgcgggtgat gtcgattgcc
      181 gacggcacgc ccataaaacc gagcagcgga atggtgacgt tggggttttc accgagggtg
      241 atttcgcgca tctgacggga gaaatcaata gcttcttcca ccgtgccgcc caccagcgcg
      301 acgatagcgg gcgcggtcgc catagcaaat ccgccaatac cgtaggtttc ggtgatggcg
      361 ctgtcgccga tatccagccc cgaatcttcc ggcttataac cggcaaacat cgggccgatc
      421 acctgctgcg ccgggccggt aaaccattgc cccggcaggc cgctgacccg caggccgaac
      481 tcgacgccgt tacgcgccat ggtggtgacc acggtgctgt attcgatgcc atgcgccgca
      541 tccatcgccg ctttacacat cgccatccac gtcgggccgg agaagtagtc gctgctggcg
      601 acaaactcaa acacttcgcg ctgttgctcg acggaataac ccgcctgaat aatccccggc
      661 gtcagcgcct gaatcagcag tgtcgtcccg gcgttattgc ggttatggca ctcatcgccc
      721 atatgcagcg cctgcgccag cattaagcgc agatcgattt cgccgataat tttcatcgcg
      781 tcgcgcagta ttggtccctg cacatcacgc atccagttca ggcggtcaat cacgctctgg
      841 tcgttagcgc ccatacgcaa aatcttcgcc atctgctcgc tcatgttggt ataagcgatg
      901 ttgccgtagg ttttgttttt tacgatgtgc ataaacatcg aggccgaggt aacacccgcc
      961 atcgatccca cgcagtcgtg ctcgtgacac ggcgagaagg tgatctcccc ggaagccgcc
     1021 agttcagccg cctcgtcgag atctttcgcc agtccttcga acaccagcgc tccggtgacc
     1081 gcgcctttca tcgcgccgca cattttttcc caggtgatcg gcggcccggc gtgaagaatg
     1141 gttttcgccg tcatgcccgg caccacgtta atcgcctgat caaaaccaat cagcaccgga
     1201 tgcgactgga taatacgctc cagcgcctgc tggttagcgg cggcgatttt gtccgccagc
     1261 ggcgaatcgg caatgttatc cagcgcctgc accacctgca tattgccctg ccccggcggc
     1321 gtccagtcga gttgagtcac ttctacatgc tgctttttca ggtcatcgct aaacatggcg
     1381 atgccgacgt taataacgtt caatggttgg ctaaacagtg actggctcat caggcttcct
     1441 cccctttgca gataaattca cgcgccagca atccggtatt ggtgctgctg ctcgccagaa
     1501 tcactccggc atcaagcagc atctggcttt gttgttctaa cgatggcgta tcaagatcgg
     1561 tacccagcac ataggcgaga atgatcaact cgcgtccttc ggcagcggcg atcgctttcg
     1621 cttctttgat cgtctcgatg gtggagccga ccggatcttc atgcgatcca aatccgagca
     1681 caaaatccat cacgatcacc gccacttctg gatcgcgcgc ctcttcgatc aagcgactga
     1741 tgcggttggt ggggtcaatc atcgggtgcg gcttgccgtt ggtgaagtcg tcatcgccaa
     1801 agtcgaggaa ggtgtgtttg atgctgcggt tgatatcttt caggcggaat tccggatcgg
     1861 gctgaatgtt gctgtagaca tcgccatgtt tttccatcac cgcgaacatg gtttcgtcgc
     1921 acagcgtgcc gccgcagaac aggccacgaa tgtatttctg ctgcggttgc agacgcgcac
     1981 gcacatccgc aatcaacggc tggttaagcg tatgcaggtc gagattttcc tgtttcacgc
     2041 cggagagcat caccgctttt agcgctgcct ctttggtgcc gcgggcaaac tgtagcccct
     2101 gctcatccac tggcgtttcg ccacgatcga ggaagcagac gaccaccggc ttgcggcagg
     2161 cgcgcgcacg ttccagcact ttgcgggcca ccgcaggcgc aggcggtttg gagataagcg
     2221 caatgatttc agtttgcgga tcgttttcca gcatcccgat ggcgtcgagc atcatcaggc
     2281 cgccgatttt ctcgctcagg tcgcgcccgc cggtgccaat cagttgcgaa acgccgccgc
     2341 caaattcatg aatgcggacg ctcaactcct gactgccggt gccggatgcg ccaacaatac
     2401 cgatgttgcc gcgacgcacg gcgttaccaa aacagagcgc cgcgccgttg ataatcgccg
     2461 tgccacagtc tggccccatc atcagcagcc ctttttcgtg ggccagttgc ttgagcgcca
     2521 gttcatcttc aactgagacg ttatcggaaa acagcatcac gttgagatcg ttttgcagcg
     2581 cctgacgcgc ttcgcgagcg gcaaacagac cgttgaccga aatcaccgcc aggttacttt
     2641 ccgggatatg ctttttggcg ctgccaatag tggcgtaacg cgcctcgtgc gagccgcttt
     2701 gcgctttggt gttgaacagt tcttcaatct ccaccagcaa ctgctcgttg tccgcacccg
     2761 atttaccatt gatgacaatc atcaggtcgc cgtttttcgc ctgctccagc tccggcgtca
     2821 gcagtcctaa attcttcagc acgcctttat tcatttcggt cgccatcgcc acaaatgcct
     2881 gctcgacgcc gtcgagttta tttgcacgcg tggagataga catcagcgag acagaatcaa
     2941 aataggtatt cggtttaatg actattttaa ctgacataat tccctccgta ggtttggctc
     3001 agcgcacaac catcggccat gccatacagc atgtcgcagc cggaactgga gccaatatgt
     3061 ttaatttttt ctattgcctg agttgcgttc ccagggatgt cataaataat gttgtgaata
     3121 aaacgatgaa tattttcccg gcagcgttgt tgtaatgcgg cttccagcgt tatggcactt
     3181 aataatgtgg tattattttt gccgcgttgc agaccgagat aaaattcctc tttgtatttt
     3241 tccgccggat gcccgggaat aaataaaata agcgccagac ctgttaaata atcgtcggct
     3301 gatggcgtaa ggccaatccc taaccccatc atactggcga ccgctgaggc gatattttgt
     3361 ttatcgttta cggcctgaat aactgcccgt cgtttaatat gtaattcttt atttaacgcc
     3421 tgataaaacg gattatcgcc gcgataatta aacagggttt cattttcccc caggtgctga
     3481 tgaataatgt cgtgccactg tagccaggcg ataaggtgaa aattcacagc ggtcatttgc
     3541 aaggttgggg acagccaacg ccgacaacga ctcatctcta tatgaaaatg ttgaccaacc
     3601 gtaatacctt gatcgtcaaa ctgaacctta tcaccatgcc ggaacagatc atcaaagtga
     3661 gtgagtgcca accgacaact gtttggcgca ttgtcgtact cttcacaaag cagcgtcagc
     3721 aactgctggc gagccgggag tgcgatattt accgctctgg caaagacctg ttcaacctgt
     3781 ccttgccccc gctgcgccag aaaatacgca tccgcagtta gcgcccacac acaccgtctt
     3841 tctggtgtgt tgatagcaag cctcccggtt tatgcacctg cggcaatcag taactgcgca
     3901 atctcttcaa agccccgttc ccgcgccagt tccagtggcg ttttgccata tttatcggtc
     3961 agatgcgggc tggcaccgtg ttccagcaat aactgcacaa tcgcctgctg tttaatacca
     4021 ccatcattaa gcacaatcgc ttccagtagc ggcgtccagc ctacatggtt ggtctggtta
     4081 acgttaatct ccgtatgcgc caaaagctct tttacaatac ttaaatggcc tttttcacag
     4141 gcaggcgtca ggccgacacc gccaaaacgg gttacgcaat taagatcggg tttagccggt
     4201 aaaataattc gtagtagcgt taaatcatcg ttcagacagc taattaaaaa aggatttaaa
     4261 caggtatgat cttgcttatt aatatccgct ccggcatcaa ttaatgcctg aacgcaagca
     4321 tattgctgat ataaacttgc cagcgtaatt gccgttttcc cctgacgatc gcaggtatta
     4381 atatcgacac ccagcgcaag gcaggttttt actttatcga tatcaccctg ttgtgcagcc
     4441 aataaatagt cggcaggtag attttttata ctcattcaca ttcccctggt ttttgtggct
     4501 gttttcgatg aattgagaat agcaacgata atctgataaa agaacccgct taaatttaat
     4561 tcgccgtatc tcgccttttt gttgaataat attcaacaat cttttcgcag tgtgcggtag
     4621 tgcaatattt gtttctttga cccgataccc tttcactcaa tcaggtttta tttctctata
     4681 gtctgcacat gtaagagtag ctttcgtcca tgtctaacca gaggggaaca agatgaacgg
     4741 actcacggca acgggtgtca ccgtcggtat ttgtgcaggg ctttggcagt tagtgtcttc
     4801 acatgtcggg ctctctcaag gatgggaatt attaggaacc attggcttcg tcgccttttg
     4861 cagtttttat gccgcaggcg gcggtaaatc ggggtttatc agaagtctgg cggttaatta
     4921 ttccgggatg gtgtgggcgt ttttcgccgc gctgactgct ggttggctgg cctctgtaag
     4981 tggactgtcc gcgttctggg cgagcgtaat tactaccgta cccttctcgg cggtagtggt
     5041 ctggcaaggg cgtttctggt tactgtcctt tattcccggt ggctttctcg gcatgacgct
     5101 gttttttgcc agcgggatga actggacggt gacgttactg ggttttctgg cgggtaactg
     5161 cgtaggggta atttcagaat atggtggaca aaaattaagc gaagcgacga ctaaacgcga
     5221 cgggtattaa aagttattca aaaatgatta tctatcttta accgatacct ctacagtcat
     5281 agctggagaa attcatagta gataatggcg agaatggttt aatgaactca atttttaccg
     5341 aagagaatct gttggcattt actaccgctg cgcgttttgg cagcttcagt aaagccgcag
     5401 aagagttggg tttaaccact tccgccatta gctacaccat taagcgtatg gagacggggc
     5461 tggatgtggt gctgttcact cgcagtaccc gcagcattga gttaacggaa tccgggcgtt
     5521 attttttccg caaagctacc gacctgctga atgattttta tgccatcaaa cgcagtatcg
     5581 atactatttc gcagggcata gaagcgcgtg tgcgcatttg tattaatcag ctgctttata
     5641 cgcccaaaca caccgcacgc ttgttacagg tgctgaaaaa gcagtttcct acctgccaga
     5701 ttaccgtcac caccgaagtg tataacggcg tctgggatgc gattattaat aatcaggcca
     5761 atatcgccat cggcgcaccc gatacactac tggacggcgg cggtattgat tacactgaaa
     5821 ttggcgcgat tcgctgggct tttgctatcg ccccggatca cccgttagcc tttgtgccgg
     5881 aacccatcgc cgaaagccaa ctgcgtctct accctaatat tatggtggag gacaccgcgc
     5941 atacgattaa taaaaaagtg ggctggttgc tgcacgggca ggagtcaatt ctggtgccag
     6001 atttcaacac caaatgtcag tgccagattt taggtgaagg aattggtttt ttgccggatt
     6061 acatggtccg tgaggcgatg acgcaatccc tgctggttac ccgacaaatc cataatccgc
     6121 gccaggattc gcgcatgtta ctggcgactc agcattctgc tacgggtcag gtgacgcagt
     6181 ggattaaaaa acaatttgcg ccaaatggca tattaacggg gatctatcag gatttactgc
     6241 atcgggagaa ttaatcgctg ccaacctccc gatgcgctgt gaatagcggt ttacatcaac
     6301 cacctgcttt cattacccat tcagagataa atttattacc cggtactggc ggagagtaca
     6361 aatagccctg caaaaagtga acgcctttac cgatcattaa atccgcctgc tcctgggttt
     6421 ctaccccttc cgccacgata ctcagaccag gcttacgcgc tagttcgaca atattgtcca
     6481 caatatgacc ggagatttcg tcgacactcg ccatttgcac aaatgactta tcgatcttaa
     6541 taaaatcgac cgggaacgcc tgcaagtaac gataggtcgc ataacccgta ccaaagtcat
     6601 ccagcgcaaa ggtaatgttg tgctgatgaa ggctgtcaaa tatcgctctg gcttctggcg
     6661 ttaccggaat agggttacgt tccgttagct cgagaaccag cttgatttta tcgttaccta
     6721 atttattaac caggttcaga cactcttttt caaatcccgc tgccaaaaaa caacccgccg
     6781 agacgttgat gccaatatgg aaattgtccg gcagcaaatg ttttaccggc atcagaatat
     6841 ccgcagtctg tttcatcagt tggcgggtca ttatgacaat aagaccggat gactccgcca
     6901 taggaataaa ctgatccggt gggataattc ccgtttgtgg atgttcccag cggacaagca
     6961 cctcacagcc cgtcagtacc ccagtctgcg cgcagaaaac cggttggatc cacggtttga
     7021 attcgtggtt ttccaacgcc agactgatgg cttcaggcgt gacgatatgg tgatagtgat
     7081 tatcattaat atatctgtga ctattactcc ccgtggcgga tatgatctcc ggattgtact
     7141 gaaagaaaat atcgcgccag aaagtaatat cgctctgaat ccccagtttc tcaaagagct
     7201 gtttcttttg atgtgaaatt gtctttgcac tgcgatttct gtactgtgat atttctgtta
     7261 cagacattcc ctgcatcagc agcttgagta gcagtcgttc tttctcggat acgctaccag
     7321 gcaatgacaa atgaaccgtt gtaccgaact cttgcagaaa aacacgaaaa tcacatgaat
     7381 tcatgaacac acctttatct tttatcgctg ttcagtatga gtgattacct gtcgattcct
     7441 gagcacacat aaggggtaac cataaacatc agcgatgcca tacgtataac atcccacatc
     7501 actatcaaga agattatctt tacctctgat gctcgctcaa aaacagttac gctaaatacc
     7561 ttaaaaaggt aatcacaagt caaactcagg agataatcac gcaagaatgc aatattaaaa
     7621 ttcagatact catttttggt aataaagaag tagttggact atttcaatat ttataatttc
     7681 ccactacata ctggcatcca ttcatgagca aaggcgcatt atatgaattt aacaatccag
     7741 atcaactgaa aatacctctc cctcataaac acatagcgtc aacattcaat gacataatga
     7801 gtaaagatgt tggttatgca tacgtatcat tactctatgc ctgtccctta aaaacccact
     7861 cattaagact gaatccattc agcaaatgat aaccaatatt atagcaatgc caataaaaac
     7921 aatatttaaa acattcaata aaatatcaca cacagataat taaaccttaa ataacaaatg
     7981 gttgcgttaa tttcagttga tttacgatac acatttctca tgaataatgc gtcatttcaa
     8041 atgatcagca atatttacat taaataagac taaacttatt gaacattcgc aacagcccaa
     8101 atgtaggttt tgggctgcaa tagttctttt cttttttatt atctgttgtc tggtaatcat
     8161 caatagatac taagggctta accgtataga tataacgtat caagaggtag gagaaacagc
     8221 gccatatgca gacatggcgc ggttttatgc aataacaatc acgcgtccgg gaacatcacg
     8281 ctatggcccg gcgcttcacg atggagatga ataaagttaa ggtgccgctc gtactggtca
     8341 agaatatcgg tgatcacctg ctctttgctg tagtccatca ggtcgttgcc ctggctgcct
     8401 tctaacagga aggtttccag ccggtagtag gtcgatttac cgctgcgtgc gcggtaggta
     8461 aagcccggca ccgaatattg ctgcggccaa atctgataga caaagttttg ctcttcgccc
     8521 atatgcacca acaaatccag atgacccaac tgctgtccct cttccggtgg caggcttttt
     8581 agctccacgt acgcgccgcg caaccgcaac tcctgcgcca cttcttccat tgccgggtaa
     8641 cagaccgtct ccatcatctg tttagtgtaa cgcgtgcccg gataattcat caggcgcgag
     8701 agacgttttt tccagctcag gcgatcctga agccccagcg gtcgcggtgc ggtatcgcgg
     8761 ttggcacttt cacggcggta atcttctacc ttcagagatt tatacaaccc cgccatcacg
     8821 aagaagatca caaagctgaa cggcagcccc ataatcaccg tggtgttttg cagcgcggat
     8881 atcccgttag tcatcagcat gccgagcgtc agcaggccaa tcgccaccga ccagaagacg
     8941 cgcagccagc cgggggcgtc gctgttgata tctttaagct gcgaggtgaa attccccagc
     9001 accagcgccc cggagtccgc cgaggtcaca taaaacagca ggccagtaat ggtggcgacg
     9061 gaggcgctaa aggtaaacgc cggatactgc gccagcaggc tgtagaagcc gcgctccgga
     9121 tggaccatcg cttcctcggc aaatgccgcg ccgccgtgga tgatttcata cagcgcgcta
     9181 ttgccgaaca ccgagagcca taacagcgtg aaggtaaacg gaataatcaa cgtgcccagc
     9241 acgaactggc gaatggtacg cccacgcgag atacgcgcca ggaacaagcc gacaaacggc
     9301 gaccatgcca cccaccatgc ccagaagaag agcgtccagt tattcatcca ctcaaccgga
     9361 cggtcgaagg caaaactgtt gagcgtcatg cccataaagc gattcacata gtcgccaaca
     9421 ttcagcacca gtgcattaag caggaacgaa gtgtcgccca taaacaatac gaacaggatc
     9481 aatcccagcg ccagcgcgac attaagctcc gataacacgc gaatgccctt atcgacaccg
     9541 gaggtgacag agatcgtggc gattatcacc gacaaggcga tcagtgccgc tttcgccgcc
     9601 atcgaatcgg gaatatcaaa cagtacgctc aagccatagt taagctgcac cacaccgata
     9661 ccgagcgtag tggcaatacc gaagatagtg ccgatcaccg ctgcaatatc cactgagtga
     9721 cctatcggcc cgttaatccg tttaccgaag atcgggtaca gcgccgagcg gatggtgagc
     9781 ggcaaattat aacgatagct aaagtatccg agcgccatgc ccatcagcgc atacatcgac
     9841 cagccggtta agccgtagtg aaacagcgtc cagaccatcg cctgacgcgc ggcctcaatc
     9901 gtctgtcccg cgccttccgg cggctgcata tactgcgtta ccggttcggc tacggagaag
     9961 aacatcaggt cgataccgat cccggcagca aacagcatcg ccgcccaact cagcaggctg
    10021 aattccggtt tggattgttc tggcccgagc ttcaccgaac caaaacgcga acaagcgata
    10081 cagaccacaa agacaatata gagcgttgcc gccagcagat agtaccaacc gaaggtttta
    10141 gaaacccagt ccagcgtgcg gccaatccac agggccgaga agtcgcgaaa caggatcgtt
    10201 gtcagggaaa acaacaaaat cagtccggcg gaggtgtaaa acaccaccgg attgattttg
    10261 tccttttccc tgctgtgtga aaggtctgtc atccagtatc cccactgtta ttgttaccat
    10321 ttaaaatcaa attcgtaaca attaagacac attttatatt gaacgtccaa tcaataaccg
    10381 ctttaataga taaacaccgc tgatgaatgg agtggcgaaa atgcccaaat tggggatgca
    10441 gtcgatccgg cgcagacaac tgatcgacgc cacactggaa gcaataaatg aagtgggcat
    10501 gcacgatgca acgatcgcgc agatcgcccg ccgtgcaggc gtttctacgg ggatcatcag
    10561 ccactatttc agggacaaaa atggtctgct ggaagcaacc atgcgcgata tcaccagtca
    10621 gctgcgtgac gcggttttga atcgattata tgcacttccg cagggcagtg cagagcagcg
    10681 attacaggcg attgttggcg gaaacttcga tgaaacgcag gtgagcagtg cggcgatgaa
    10741 agcctggctg gcgttctggg ccagcagtat gcatcagccg atgctctatc gtttacagca
    10801 ggtcagcagc cgccgcttgc tgtcgaatct ggtgagcgag tttcgtcgcg aattgccgcg
    10861 cgaacaggca caggaagcgg gctacggcct ggccgcgctg attgatggat tatggctgcg
    10921 cgcggctctg agcggcaaac cgctggataa aacccgcgct aattccctga cccgccactt
    10981 tatcactcag catctaccca ccgattaacc gaggagacgt gatgtcccga atggcagaac
    11041 agcagcttta tatacatggt ggttatacct ccgccaccag cggtcgcacc ttcgagacca
    11101 ttaacccggc caacggtaac gtgctggcga ccgtgcaggc cgccgggcgc gaggatgtcg
    11161 atcgcgccgt gaaaagcgcc cagcaggggc aaaaaatctg ggcgtcgatg accgccatgg
    11221 agcgctcgcg tattctgcgt cgggccgttg atattctgcg tgaacgcaat gacgaactcg
    11281 caaaactgga aaccctcgac accggaaaag catattcgga aacctcaacc gtcgatatcg
    11341 ttaccggtgc ggacgtgctg gagtactacg ccgggctgat cccggcgctg gaaggcagcc
    11401 agatcccgtt gcgtgaaacg tcctttgtgt atacccgccg cgaaccgctg ggcgtagtgg
    11461 cagggattgg cgcatggaac tacccgatcc agattgccct gtggaaatcc gccccggcgc
    11521 tggcggcagg caacgcaatg attttcaaac cgagcgaagt taccccgctt accgcgttaa
    11581 agctggctga aatttacagc gaagcgggcc tgccggacgg cgtatttaac gtgttgccgg
    11641 gcgtgggcgc ggagaccggg caatatctga ccgagcatcc gggcattgcc aaagtgtcat
    11701 ttaccggcgg tgtcgccagc ggcaaaaaag tgatggctaa ctcggcggcc tcttccctga
    11761 aagaagtgac catggaactg ggcggtaaat caccgctgat cgttttcgat gatgcggatc
    11821 tcgatctcgc cgccgatatc gccatgatgg caaacttctt cagctccggt caggtgtgta
    11881 ccaatggcac ccgcgtcttc gttccggcga aatgcaaagc cgcatttgag cagaaaattc
    11941 tggcgcgcgt tgagcgcatt cgcgcgggcg acgttttcga tccgcaaact aacttcggcc
    12001 cgctggtcag cttcccgcat cgcgataacg tgctgcgcta tatcgccaaa ggcaaagagg
    12061 aaggcgcgcg cgtactgtgc ggcggcgatg tactgaaagg cgatggcttc gataacggcg
    12121 catgggttgc accgacagtg ttcaccgatt gcagcgacga tatgaccatc gtgcgtgaag
    12181 agatcttcgg gccagtgatg tccattctga cctacgagtc ggaagacgaa gtcattcgcc
    12241 gcgctaacga taccgactac ggcctggcgg cgggcatcgt gacagcggac ctgaaccgcg
    12301 cgcatcgcgt cattcatcag ctggaagcgg gtatttgctg gatcaacacc tggggcgaat
    12361 ccccggcaga gatgcccgtt ggcggctaca aacactccgg cattggtcgc gagaacggcg
    12421 tgatgacgct ccagagttac acccaggtga agtccatcca ggttgagatg gctaaattcc
    12481 agtccatatt ctaaccagga ggtttatttg caatttgact acatcattat tggtgccggc
    12541 tcagccggca acgttctcgc tacccgtctg actgaagatc cgaatacctc cgtgctgctg
    12601 cttgaagcgg gcggcccgga ctatcgcttt gacttccgca cccagatgcc cgctgccctg
    12661 gcattcccgc tacagggtaa acgctacaac tgggcctatg aaacggaacc tgaaccgttt
    12721 atgaataacc gccgcatgga gtgcggacgc ggtaaaggtc tgggtggatc gtcgctgatc
    12781 aacggcatgt gctacatccg tggcaatgcg ctggatctcg ataactgggc gcaagaaccc
    12841 ggtctggaga actggagcta cctcgactgc ctgccctact accgcaaggc cgagactcgc
    12901 gatatgggtg aaaacgacta tcacggcggt gatggcccgg tgagcgtcac tacctccaaa
    12961 cccggcgtca atccgctgtt tgaagcgatg attgaagcgg gcgtgcaggc gggctacccg
    13021 cgcacggacg atctcaacgg ttatcagcag gaaggttttg gtccgatgga tcgcaccgtc
    13081 acgccgcagg gccgtcgcgc cagcaccgcg cgtggctatc tcgatcaggc caaatcgcgt
    13141 cctaacctga ccattcgtac tcacgctatg accgatcaca tcatttttga cggcaaacgc
    13201 gcggtgggcg tcgaatggct ggaaggcgac agcaccatcc caacccgcgc aacggccaac
    13261 aaagaagtgc tgttatgtgc aggcgcgatt gcctcaccgc agatcctgca acgctccggc
    13321 gtcggcaacg ctgaactgct ggcggagttt gatattccgc tggtgcatga attacccggc
    13381 gtcggcgaaa atcttcagga tcatctggag atgtatctgc aatatgagtg caaagaaccg
    13441 gtttccctct accctgccct gcagtggtgg aaccagccga aaatcggtgc ggagtggctg
    13501 tttggcggca ctggcgttgg tgccagcaac cactttgaag caggtggatt tattcgcagc
    13561 cgtgaggaat ttgcgtggcc gaatattcag taccatttcc tgccagtagc gattaactat
    13621 aacggctcga atgcagtgaa agagcacggt ttccagtgcc acgtcggctc aatgcgctcg
    13681 ccaagccgtg ggcatgtgcg gattaaatcc cgcgacccgc accagcatcc ggcgattctg
    13741 tttaactaca tgtcgcacga gcaggactgg caggagttcc gcgacgcaat tcgcatcacc
    13801 cgcgagatca tgcatcaacc cgcgctggat cagtatcgtg gccgcgaaat cagccccggt
    13861 gtcgaatgcc agacggatga acagctcgat gagttcgtgc gtaaccacgc cgaaaccgcc
    13921 ttccatccgt gcggtacctg caaaatgggt tacgacgaga tgtccgtggt tgacggcgaa
    13981 ggccgcgtac acgggttaga aggcctgcgt gtggtggatg cgtcgattat gccgcagatt
    14041 atcaccggga atttgaacgc cacgacaatt atgattggcg agaaaatagc ggatatgatt
    14101 cgtggacagg aagcgcagcc gaggagcacg gcgggatatt ttgtggcaaa tgggatgccg
    14161 gtgagagcga aaaaatgagt cgtgatgtga actaacgcag gaaccgatca gattgagagt
    14221 taccgttcca gagaggggga ccgaatcctt atataaacac tgaggtaact ctcatgcttc
    14281 atatccagta ttcatgatgc gggctttttg ctgatattta acccgaacat tcctgataca
    14341 gactattaat acaaccttat ctgcgacaga ttttattctc tggatatatt atgcgtgaac
    14401 aaatcaaaca ggatatcgat ctgattgaga ttttatttta tctgaagaaa aagattcgtg
    14461 ttatcctttt tattatggct atatgtatgg ctatggtgct gttgtttctg tatatcaata
    14521 aagacaatat aaaagtgatt tacagcctaa aaataaacca gacaacgcca ggtatacttg
    14581 ttagctgtga tagcaataat aattttgcct gtcagactac aatgactgaa gatgttattc
    14641 agcgaattac tacatttttt cacaccagcc cagatgtcaa gaacagagaa ataaggctgg
    14701 aatggtcagg agataagaga gctttaccaa ctgctgaaga ggaaatatct cgcgtgcagg
    14761 cctctattat caaatggtat gcgtcagaat atcataatgg caggcaagtt ctcgatgaga
    14821 tacaaacgcc ttcagcaatt aacagtgagc tttatacaaa aatgatatac ctgaccagga
    14881 actggtcatt gtatccgaac ggtgatggct gtgtaactat aagctcacca gaaataaaaa
    14941 ataaataccc tgctgccatt tgcctggctc tgggattttt tctaagcatt gtaatttctg
    15001 taatgttttg ccttgtcaaa aaaatggtag atgaatacca acaaaactct gggcagtaat
    15061 aaacaacatt ctgatagaga gtgcctgtat aaagctgaag tgacaatgac cgggaaggga
    15121 acccgttttc aggaatggat gcctgtcttt ttcactgtaa atatcctggc attggtaata
    15181 ccaggatatt tacagaagag cagcataacc atgacggaac ggggttaagt caggttgtcc
    15241 taaacgctat tttcaacctc gtatgccttc ttcaggttta tgtccagact tcatatctct
    15301 ctcaacaatc ctcaataatc agatacaccg ctttcaccgg gccatgaact ccgacgactt
    15361 tgataagctc aatatccgcc gttgaactgg ggccgctaat gatgttaatg caggaaggca
    15421 ttcgttcacc ggcctgcgct ttctgatgca atttttctgc gagttgcgct acacgcggca
    15481 ggatagtgct tttacgcagg ataaaaagag aatattccgg gagcaggctc aatgaacgcc
    15541 cgcgctcggc ggcggaaaaa agaaccacgc ctcccgattc ggttaaacca tattcagcat
    15601 acacaacacc cactttagcc tgctctgcct gcgagatatt ctcggcacct ttcgccggat
    15661 cccaaacaac ggcattgcat tcctgctgca aacgttcgct aatccccaat tcctccagcc
    15721 tcgtgtcacc gctaatcacg accgactgat ctcccagctc tttacacaga cgtattgcag
    15781 cttctgccgc cttcgcctcg ctggtcagct cacagcgcgt caacataaca tcgctggcaa
    15841 actgaataaa cgcgtcacag cgctgctgtt ggttaagttg ggtaagccgc tcgttagcat
    15901 agttgttaag cggcgcatct tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct
    15961 gagcaacgtt attcaaaaat tcgcctcgat tatccattct ttttctcctg cgcctgatgt
    16021 ttcttaaacc aactacggaa actctctccg tcagcttcag gaagatcgcg tgcttccatc
    16081 cagtcgctaa tcgcgccaaa tttgagtggt gttttgccgc cattgataaa ccagcttgcc
    16141 gcatgagcac cggccatcat cccgactttc cacaatcctg gatgactatt ggcataagcg
    16201 aacattttta tcgcccgttg ctctgctttt gcggtgatcc ctttttcagc catcacccga
    16261 cgatgacgca aaatcagttt tgacagcgga atacgcaccg gacacacgtt gtcacaagct
    16321 gtgcataaag agcaggcgta gggtaaatct ttaaaatctt tatagccgcc aagtagcgga
    16381 gaaatcaccg caccaattgg ccctggataa atagagccat atccatgacc gccaatatgg
    16441 cgatatgccg gacaagtatt catacaagcc ccgcagcgaa tacagcgcag cacatcccga
    16501 aattcagagg ccagcacctc agaacgcccg ttatcgacaa taaccagatg aaactcttca
    16561 ggaccatcaa cgtgcccagc ttcgcgcggt cctgtcagcc aggtgttgta tcccgtcaaa
    16621 cgtgcaccaa cggcactgcg cgccagcatg gtgatcaata catctacctc ggcaaacgtg
    16681 ggggcaatac gctccattcc catcactgca atatgcgttt taggcagcgt ggtacacatt
    16741 cgcgcattac cttcattggt caccaggcat accgaaccgg tctctgccac tgcgaaatta
    16801 cagccggtaa tacctatttc agcactgagg aaatcttcgc ggattttttg ccggatgaat
    16861 aaggtcatcg cttcaggcgt ttccggcccc tcatagccca gacgttcgtg tagcactcga
    16921 cggatctgat ggcgatcttt atgaattgcc gggaccacaa catgagatgg cggatcttga
    16981 tccagctgga gaatatattc acccagatcg gtttcaatca cctgaatgcc agcatcctgc
    17041 aacacatgat tgacaccaat ctcttcggtc accatcgatt tagatttcac caccttccgg
    17101 gcatttttgc gttgggcaac ctgtaaaatg tagcgggtag cgtcttcttt ggttcttgca
    17161 aaatagacgt gaccgccgtt ttgcgtcact ttttctgaga gctggtacag ataagcgtcg
    17221 agattactca gaacatgatc acgtatctgg gcggcccgat cgcgccactc ctcccagtgc
    17281 cccaattcat cgaccatttt ttgccgattt gccccaatac gctgctgcgc gtttgccacc
    17341 gctttgcgca tgatcggatc ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta
    17401 ctggttttga tcgacatctt tatatcctca gcggctcatc aacacttcag caatatgcat
    17461 cactttgact ttctgccctt cccgttgtaa tcgcccactg atgtttagca ggcaactcac
    17521 gtcagcacca attaaatact cagggcggac ttccatcagg tgcgcaacct tttctttcac
    17581 catctcgccg gatatttcgg ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt
    17641 atcctgttca gcaaaggtca acagctccag tccacgcaca tttttcagca gcgtaagtgg
    17701 ctcgtccttc actcccagct tacgggccag gctacaagat gggtgataca ccgctctccc
    17761 ttgcaaactg gcacctacat cgactacccc taatttatta acaataaaag aggtgagatc
    17821 ctgcatacgc gcggcaacct ttgcggcacg tgatgcccat tcaggttcat ccgccagata
    17881 cgtcgggtaa ctttttacgg cataggtgca agagccagcc ggtgaaataa tgggatcgtc
    17941 gttatcctcc agtgcggcga tcagattttt catccctgga atcgcttctt tgatataacc
    18001 gctattgatc gcaggctgac cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc
    18061 gagtttttcc agtagcagca cggagtctcg tgccattctt gatttcaggg cgtcaccaat
    18121 acaggtgaca aagaaattga cattcacaac taatactcca ttacttcatg cccatttatg
    18181 cccgatgttt cccccccacc gggggatcaa tcatcaataa atatggcgtt cttttttaat
    18241 tggctagcaa cataccaccc ctcatttgta tgacaaatga taatctttca tccaaacgtc
    18301 aaatgcaata actaatttta tttctttcgg catcgctcac attttatggg gaaatcgacg
    18361 atcatttttg gcataagaaa tccatttaaa aatggctaca tcccttatac ctattcagag
    18421 ctacttattc aggggttatt gagatataca ctgatggtgt acaaaaaaat caccgacaat
    18481 aatcacgtat caagtaatag tcgggatttt atttaattga agcaatttag tttgcatttt
    18541 tatttaaaaa ctgagctaga gtccatcaga aaaaatatat aaagcccaaa ccaccgttgg
    18601 aaatagaaat agttgaaagg ctcacaactc ccggaatgcg gattatcttt gccctgattt
    18661 ctggcggttt tattcagggt gcaagctgtc tgaccctttc ccgatattct cccggggtac
    18721 aaccaaactc gcggacaaac gccttgtgaa aagatgattc actggcatag cctactgact
    18781 cagcgatcac cacaacaggg agcgtttccc gggaaaacat ctgggccgct atttgtagac
    18841 gcaactttgt taatacagcc agcggcgtgg ttccggaaac atcacggaaa agctgggcaa
    18901 aacttgcccg ggacatgtga gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg
    18961 gcatttccag catttgctgt attaccgcac caagacgtgg atgcagaagc aaactgagaa
    19021 tgtttttctc agtattaacc tgtgcaatcc actcacgcac cgcgagggta aagaatgtag
    19081 cgcagatctg gctacacagt gcatccactc ccggcattgc cgatctggat tcctgttgta
    19141 aaaacgggat cgcctccctc agccagttat attccacgct gtggttaacc ggtgctaaaa
    19201 acagcgtttc cggcaaagac gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc
    19261 cgcagacaat acaggtcgat tcattatcga catgactcag gcgatgagct gaattttgtg
    19321 gtagcaggac cacatttccc gggcgtaatg taaaaatctc ccccgtcggc atttccagct
    19381 tcgccgctcc ttgcgttaac gcatgccaac gaataaccga taattccccg gcaccatgtg
    19441 gaagctgcca gtcacttcct aacacgcaat tcttatcgat cgttccttgt ggagcgttaa
    19501 gcatcaacaa acggctaagg gcatccatat cagactcctg agtggttttt gattacaaac
    19561 accaacaata ttagtaaata actaatctaa taccatcaaa tagttaatca tgatttttgt
    19621 tttgtgattc tgattaagaa aaagaaaata tcagacgata agacaaaata tcaagcgttg
    19681 tgaagaaatg ttatttgctc ttttgcgtct atagtcatga tgtcaaatga acgcgtttcg
    19741 acaggaaatc atcatgaata aatatcaggc agtgattatt ggttttggca aggctggaaa
    19801 aacattagcc gtcacgctgg caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa
    19861 tgcaatgtat ggcgggacct gtattaatat cggctgcatc ccaaccaaaa cattggttca
    19921 tgacgcacag cagcacacag attttgtccg tgccatacag cgtaaaaatg aagtggttaa
    19981 ttttttacgt aataagaatt ttcataatct tgcggatatg cccaatatcg acgtgatcga
    20041 cggccaggcg gagtttatca ataatcatag cctgcgtgtt catcggcctg agggaaatct
    20101 ggaaattcat ggcgagaaaa tttttattaa taccggtgca caaaccgtgg ttccgccaat
    20161 tcctggaatt accaccacgc caggagtata tgacagcacc ggattactta atctaaaaga
    20221 attgcctggg catttaggta ttttgggcgg cggatatatt ggcgttgagt tcgcctctat
    20281 gttcgctaat tttggcagca aggtaaccat tttagaagca gcttcgctgt ttttgcctcg
    20341 ggaagatcgg gatattgctg ataatatcgc gacgatttta cgcgatcagg gcgtcgatat
    20401 tatcctcaat gcccatgtgg agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag
    20461 cgagcacgcc caactggcgg tggatgcact gttaatagct tccggtcgtc aaccggctac
    20521 cgcttcgtta catccagaaa atgccggtat cgcagtaaac gagcgcgggg caattgtcgt
    20581 tgacaagcga ttacatacca ccgcagacaa tatttgggcg atgggagatg ttaccggcgg
    20641 gctgcaattt acttacatat cactggatga ttaccgcatt gtacgtgatg agttactggg
    20701 tgaaggcaaa cgtagtactg atgatcggaa aaatgtgcct tattccgtat ttatgacacc
    20761 gcccctgtcc agggttggta tgacagaaga acaagccaga gagagtggtg ctgatattca
    20821 ggtggtgaca ttgcctgtag ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg
    20881 ggtattaaaa gcgattgttg ataataaaac ccaacgtatg ttaggggcat cactgctgtg
    20941 tgttgactcc cacgagatga tcaatatagt gaaaatggtg atggatgccg ggctgcctta
    21001 tagcatatta cgcgatcaga tatttactca tccgtcgatg agcgaatcac tcaatgatct
    21061 attttcatta gtcaaataaa ctcaaaatca gacgccagaa caaatattct ggcgtctcag
    21121 agaaaagaat cttattaatt ctctgttact ctataacttc ttaatcactt cattgatggt
    21181 attttatatg tttaaaaaat ctgttttatt tgcaacacta ttatctggcg ttatggcatt
    21241 ttccaccaat gcagatgata aaataattct gaaacatatc agcgtctcgt cagtatcagc
    21301 atcaccgaca gttctggagg ataccattgc tgatatagcc agaaaatata atgcttcatc
    21361 ctggaaagtc acatcgatgc gaattgataa taattcaacc gcaacagcag tattgtataa
    21421 ataaggatgt tcacaatgga aaaatacctg cacctgttaa gtcggggaga taaaattggc
    21481 ctgacattga ttcgtctgag tattgcaatt gtttttatgt ggattgggtt attaaagttt
    21541 gtcccttacg aggcagacag cattacacca ttcgtcgcaa acagtccact aatgtcgttc
    21601 ttttatgaac acccggaaga ctataaacag tatctgactc acgaaggcga atacaaacca
    21661 gaagcaaggg catggcaaac ggccaataat acctatggtt tttccaacgg tcttggcgtc
    21721 gtggaggtga ttattgctct gctggttttg gctaatcctg tcaatcgctg gttaggttta
    21781 ttgggaggac tgatggcatt taccacgccg ctggtaacac tctcattttt aatcaccacc
    21841 ccggaggcat gggtacccgc attgggtgac gctcatcatg gtttccctta tttatccggt
    21901 gctggtcgcc tggtattgaa agatactctg atgctggcag gtgcagtcat gataatggca
    21961 gattcggcgc gggaaattct taaacaacgc agtaatgaat ccagttcaac gttaaaaact
    22021 gaatattgat aatccgaaca ctgtctgttg ttcacttttt caggcgtcag gcccccttt
//
LOCUS       DAZFZC010000086        21809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFZC010000086 DAZFZC010000000
VERSION     DAZFZC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.086"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..838
                     /locus_tag="ACHKXP_003698"
     CDS             <1..838
                     /locus_tag="ACHKXP_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO7648627.1"
                     /translation="ALRHRDIIIAKIDLFIQRITHNLWHARKKNVFLIEQITNLKEWV
                     NKYIADCTDEELNDRDFIASVVDRAIFHFAVNSICNTEGNKDATPIERYTFDVETKNG
                     LPSTVQLFYEESKDNEPLANIHLQAIGSGFLTFVNACQKYDDNSLKLFASLLISLSYS
                     SAYTDLAGAEKVNINDHNGNYLTAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDK
                     MSHLVNEGTLAPDILSKMRDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKS
                     EK"
     gene            1199..2236
                     /gene="dusA"
                     /locus_tag="ACHKXP_003699"
     CDS             1199..2236
                     /gene="dusA"
                     /locus_tag="ACHKXP_003699"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO7648628.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCNFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDIDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2370..2608
                     /gene="pspG"
                     /locus_tag="ACHKXP_003700"
                     /pseudo
     CDS             2370..2608
                     /gene="pspG"
                     /locus_tag="ACHKXP_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
     gene            complement(2774..3757)
                     /gene="qorA"
                     /locus_tag="ACHKXP_003701"
     CDS             complement(2774..3757)
                     /gene="qorA"
                     /locus_tag="ACHKXP_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO7648629.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3840..5255
                     /gene="dnaB"
                     /locus_tag="ACHKXP_003702"
     CDS             3840..5255
                     /gene="dnaB"
                     /locus_tag="ACHKXP_003702"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO7648630.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5308..6387
                     /gene="alr"
                     /locus_tag="ACHKXP_003703"
     CDS             5308..6387
                     /gene="alr"
                     /locus_tag="ACHKXP_003703"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO7648631.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVSVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6640..7833
                     /gene="tyrB"
                     /locus_tag="ACHKXP_003704"
     CDS             6640..7833
                     /gene="tyrB"
                     /locus_tag="ACHKXP_003704"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO7648632.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKVRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(8335..8538)
                     /gene="yjbS"
                     /locus_tag="ACHKXP_003705"
     CDS             complement(8335..8538)
                     /gene="yjbS"
                     /locus_tag="ACHKXP_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbS"
                     /protein_id="HFO7648633.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDFFLYIETQLKIAK
                     DFLDLEKKYQVLLQVKFFTHLLI"
     gene            8940..9653
                     /gene="aphA"
                     /locus_tag="ACHKXP_003706"
     CDS             8940..9653
                     /gene="aphA"
                     /locus_tag="ACHKXP_003706"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO7648634.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGVDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            9764..10180
                     /gene="yjbQ"
                     /locus_tag="ACHKXP_003707"
     CDS             9764..10180
                     /gene="yjbQ"
                     /locus_tag="ACHKXP_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO7648635.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            10184..10540
                     /gene="yjbR"
                     /locus_tag="ACHKXP_003708"
     CDS             10184..10540
                     /gene="yjbR"
                     /locus_tag="ACHKXP_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7648636.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(10575..13397)
                     /gene="uvrA"
                     /locus_tag="ACHKXP_003709"
     CDS             complement(10575..13397)
                     /gene="uvrA"
                     /locus_tag="ACHKXP_003709"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO7648637.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            13651..14187
                     /gene="ssb1"
                     /locus_tag="ACHKXP_003710"
     CDS             13651..14187
                     /gene="ssb1"
                     /locus_tag="ACHKXP_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO7648638.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(14286..14567)
                     /gene="yjcB"
                     /locus_tag="ACHKXP_003711"
     CDS             complement(14286..14567)
                     /gene="yjcB"
                     /locus_tag="ACHKXP_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO7648639.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            14997..16583
                     /gene="pdeC"
                     /locus_tag="ACHKXP_003712"
     CDS             14997..16583
                     /gene="pdeC"
                     /locus_tag="ACHKXP_003712"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO7648640.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(16586..16909)
                     /gene="soxS"
                     /locus_tag="ACHKXP_003713"
     CDS             complement(16586..16909)
                     /gene="soxS"
                     /locus_tag="ACHKXP_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO7648641.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            16995..17459
                     /gene="soxR"
                     /locus_tag="ACHKXP_003714"
     CDS             16995..17459
                     /gene="soxR"
                     /locus_tag="ACHKXP_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO7648642.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            18005..19354
                     /gene="ghxP"
                     /locus_tag="ACHKXP_003715"
                     /gene_synonym="yjcD"
     CDS             18005..19354
                     /gene="ghxP"
                     /locus_tag="ACHKXP_003715"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO7648643.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            19505..21154
                     /gene="yjcE"
                     /locus_tag="ACHKXP_003716"
     CDS             19505..21154
                     /gene="yjcE"
                     /locus_tag="ACHKXP_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO7648644.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(21308..>21809)
                     /gene="espX5"
                     /locus_tag="ACHKXP_003717"
     CDS             complement(21308..>21809)
                     /gene="espX5"
                     /locus_tag="ACHKXP_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HFO7648645.1"
                     /translation="PYINLFRADLSFSKLDLINFEHADLSRVNLNKATLQNINLIDSK
                     LFFTRLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSVLTKAWMFNIRLYRVNFDE
                     ASVQGMGITILRGEENISINSDILVTLQKFFEEDCATHTGMSQTEDNLHAVAMKITAD
                     IMQDAD"
BASE COUNT         5260 a         5218 c         5606 g         5725 t
ORIGIN      
        1 agcgctgcgt catcgggata taattattgc aaaaatagat ttgtttattc aaagaatcac
       61 tcataattta tggcatgcac gtaaaaaaaa tgtgttttta attgagcaaa taactaatct
      121 caaagagtgg gttaataagt atattgcaga ttgcactgat gaggaattaa acgatcgtga
      181 ctttattgca tcagttgttg accgggctat ttttcatttc gcggttaata gtatatgtaa
      241 tactgagggt aataaagatg caacgcccat agaacgatat acttttgatg tagaaaccaa
      301 gaatggcctt ccctccacgg ttcagctatt ttatgaggaa tctaaagata atgaaccttt
      361 agcgaatata catttgcaag caataggttc tggtttttta acgtttgtta atgcatgtca
      421 gaaatatgat gacaacagct taaaattatt cgcttcgctg ttgatttcac tttcatactc
      481 tagtgcctac acagatttag caggagcaga aaaagtgaat attaatgatc ataatgggaa
      541 ctatctgaca gctcagtttg aagagttatc tcaacgtgat atgaagaagt acctgggaga
      601 aatgaagcgt ctggcagatg ggggagaaat gaattttgat ggctatctgg ataagatgtc
      661 acatctggtg aatgaaggaa cgctcgcccc tgatatttta agcaaaatgc gagatgctgc
      721 accgaaatta attgactttg ctaagtcgtt tgacccaaac tcaaaggaaa agattaaaat
      781 atatacaggt acttctaaat taatttatga tttattcggg gttaaatcgg agaaataata
      841 tgtgaagttc ttcgatggta tgaaaggtat tacataaaag aacccaatgc ttattgggtt
      901 cttttttctc tatcagtgct attagcaggg agagatatca ccggagttta atgtgtgatt
      961 tttatttatc gtcgaatctg tattgtttat cattgctttt cacaacgcta acatctaata
     1021 agattagatc ttcacttcgt gagagcttca tgccttgaga ggatctcatt tttcttttgc
     1081 aatgagacag gcgcttcctg ttgttatggt atagtacccc gttattgagc ctcctgaaca
     1141 gtgatgctga ataacataac tccatgatat atcgataaaa taatctctac atttgaaaat
     1201 gcacggtaat tctgaaatgc aaaaaatcaa ccaaaccagc gcaatgcctg aaaaaactga
     1261 cgttcactgg agtggtcggt ttagcgttgc accaatgctc gactggacgg acagacattg
     1321 ccgctatttc ttgcgtctgc tttcccgcaa tacgctgctg tataccgaaa tggtgaccac
     1381 aggggcgatt attcacggta aaggtgatta cctggcgtac agtgaagaag aacatccggt
     1441 agcgttgcaa ctcggcggta gcgatccggc ggcgctggcg cagtgtgcga agctggcaga
     1501 agcgcgcgga tatgatgaga tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa
     1561 cggcatgttt ggtgcgtgtc tgatgggtaa tgcgcagctg gttgccgact gcgtgaaagc
     1621 gatgcgcgat gtggtgtcga ttccggtgac ggtgaaaacg cgtattggca tcgatgacca
     1681 ggacagctat gaatttctct gcaatttcat caacaccgtt tccggcaaag gcgagtgtga
     1741 gatgttcatc atccacgcac gtaaagcctg gctttcgggg ttaagtccga aagaaaaccg
     1801 tgagatcccg ccgctcgatt atccgcgtgt gtatcaactg aagcgtgact ttccgcatct
     1861 gacaatgtcg attaacggtg gtatcaagtc gctggaagag gccaaagcac acctgcaaca
     1921 tatggatggc gtgatggtcg ggcgcgaggc gtatcagaat ccgggtattc tggcggcggt
     1981 agaccgggag atctttggtt cctcggatat cgatgccgat ccggtggcgg tagtgcgcgc
     2041 catgtatccg tacattgagc gtgaactcag ccaggggacg tatctcggtc atattacccg
     2101 gcatatgttg ggcttgttcc agggtattcc tggcgcgcgg cagtggcggc gttatttaag
     2161 tgaaaatgcc cataaagcgg gtgcagacat taatgtgctg gaacacgcgc tcaaactggt
     2221 ggcggataag cgttaacttt tcaccaaaaa atagtcaaat tcaccacgcc ctgcgcaccg
     2281 tcgcggggcg ttttgctgtt aaatcaatag attatttttg gcatgattct tgtaatgcca
     2341 gcaagagatt tcatatttgg gagtgcatca tgctggaact actttttgtg attggctttt
     2401 ttgtcatgct gatggtcacc ggcgtttcgt tgctgggcat tatcgccgcg ctggggcgac
     2461 ggccattatg ttcctcggcg gtatgctggc attgatgatt aagttgctgc cgtggttact
     2521 actggcgatt gcggtggtgt gggttattaa ggcgattaaa gcaccaaaag tgccgaaata
     2581 tcagcgttat gaccgctggc gttactaagg gattgtgcgg atgatcacaa cctaaggttt
     2641 tatccttaga acaaaatagg aattgataat caaatctgtc actattgcgc ctctaacaga
     2701 ttcatcgtgc tgtaccctac atacagccga actataaaaa gaaagggctt cccaggtgga
     2761 agccctattt cttttatgga atcaacaggc tggaaccttg cgttgcccgg ctttccagaa
     2821 tttcatgcgc acgctgcgca tccttcagcg gatatttctg ctgctcggcg acatcgacct
     2881 taatcacacc gctggcaatc aaagagaaca gttcattact ggcctcggtt aattcctccc
     2941 gcgtggtgat atagccttgc agggaagggc gtgtcacata caacgagcct ttctgattga
     3001 gaatgcctaa gttcacaccg gtaaccgcac ctgatgagtt gccaaaactc accattaagc
     3061 cgcggcgttg caggcaatcc agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca
     3121 ccacgcgcac tttcttaccg ccggtgatct cttttaaccg ctcgaccaga ttctcttcac
     3181 gatagttaat aacctgccac gcgcccgctt ttagcgcgct ctgcgctttt tgcgcggttc
     3241 ctacggtgcc gataagtttc gcgcccaggg cttttgccca ctggcaggca atcaagccaa
     3301 cgccgccagc tgctgcgtgg aacaggaact gctcatcggg tttaatttca taggttttgc
     3361 gcagcagata ataaaccgtt aagcctttca ggaaggatgc cgcagcttgc tcaaaagaaa
     3421 ttgccgcagg cagaatcgcc gctttgtccg caataatgtt atgcacagag ctgtaagcgc
     3481 ctaacgccga ctgcgcatag actacacgat cgcctgcctt aatatgcttt acaccactgc
     3541 cgactttact cacgatgcct gctgcttcgg tgcctaatcc gctgggtagc gatggcggcg
     3601 ggtaaaggcc gctgcggata tatgtgtcga taaaattgat gccgatggct ttattttcga
     3661 cctggatttc attctccgcc ggatcggcag gagtgaactc tacggcttga agtacttccg
     3721 ggccaccgtg cttgtgaaat tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca
     3781 gggtctgctt catatggtaa agtttcgacc cattctttat ctcggtaact ccattcacta
     3841 tggcaggaaa taaacccttc aacaaacagc aggctgaacc ccgcgaacgc gatccacaag
     3901 ttgccgggct gaaagtgcct ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt
     3961 taatgctaga taacgaacgc tgggatgatg tagccgagcg tgtggtagca gacgattttt
     4021 acacccgccc acaccgtcat atctttactg aaatggcgcg tttgcaggaa agcggtagcc
     4081 ctatcgatct aattactctt gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg
     4141 gtggttttgc ttatctggca gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg
     4201 cttatgctga catcgtgcgt gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg
     4261 agattgctga agccggtttt gatccgcagg ggcgtaccag cgaagatctg ctggaccttg
     4321 ctgaatcccg cgtctttaaa attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga
     4381 acatcgccga tgtgctcgac gcaaccgtgg cgcgtattga gcagttgttt cagcagccac
     4441 acgatggcgt taccggagta aacaccggtt atgacgatct caacaaaaaa accgctggct
     4501 tgcagccgtc ggatttgatc atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg
     4561 cgatgaacct cgtcgaaaac gcggcgatgt tgcaggataa accagtactt atcttctcgc
     4621 tggagatgcc ttcagaacag attatgatgc gttctctggc gtcgctgtcg cgcgttgacc
     4681 agactaaaat ccgtaccggg cagctcgatg atgaagactg ggcgcgcatt tccggcacca
     4741 tgggtatttt gctcgaaaaa cgcaatatct atatcgatga ctcctccggc ctgacgccaa
     4801 cggaagtgcg ttcccgcgca cgccgtattg cccgtgaaca cggcggcatc gggcttatca
     4861 tgatcgacta cctgcaactg atgcgcgtac cggcgctttc cgataaccgt acgctggaaa
     4921 ttgcagaaat ctctcgctcg ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg
     4981 cgctgtccca gttgaaccgt tctctggaac aacgtgccga caaacgcccg gtcaactccg
     5041 acctgcgtga atctggctct atcgagcagg atgcggactt gatcatgttt atctatcgtg
     5101 atgaggtgta tcacgaaaac agtgatttaa aaggcatcgc ggaaattatt atcggtaaac
     5161 aacgtaacgg cccaatcggg acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg
     5221 acaactatgc ggggccgcag tacgacgacg aataataatt attttatgaa ttaggtaatt
     5281 aaagcaaaca cttatcaagg aacacaaatg caagcggcaa ctgttgtgat taaccgccgc
     5341 gctctgcgac acaacctgca acgtcttcgt gaactggccc ctgccagtaa aatggttgcg
     5401 gtggtgaaag cgaacgctta tggtcacggt cttcttgaga ccgcgcgaac gctccccgat
     5461 gctgacgcct ttggcgtagc ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc
     5521 accaaacctg tactgttact cgaaggcttt tttgatgcca gagatctgcc gacgatttct
     5581 gcgcaacatt ttcataccgc cgtgcataac gaagaacagc tggctgcgct ggaagaggct
     5641 agcctggacg agccggtttc cgtctggatg aaactcgata ccggtatgca ccgtctgggc
     5701 gtaaggccgg aacaggctga ggcgttttat catcgcctga cccagtgcaa aaacgttcgt
     5761 cagccggtga atatcgtcag ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc
     5821 gagaaacaac tcgctatctt taataccttt tgcgaaggca aacctggtca acgttccatt
     5881 gccgcatcgg gtggcattct gctgtggcca cagtcgcatt ttgactgggt gcgcccgggc
     5941 atcattcttt atggcgtctc gccgctggaa gatcgctcca ccggtgccga ttttggctgt
     6001 cagccagtga tgtcactaac ctccagcctg attgccgtgc gtgagcataa agccggagaa
     6061 cctgttggtt atggtggaac ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg
     6121 atgggctatg gcgatggtta tccgcgcgcc gcgccgtccg gtacgccagt gctggtgaac
     6181 ggtcgcgaag taccgattgt cgggcgcgtg gcgatggata tgatctgcgt agacttaggt
     6241 ccacaggcgc aggacaaagc cggggatccg gtcattttat ggggcgaagg tttgcccgta
     6301 gaacgtatcg ctgaaatgac gaaagtaagc gcttacgaac ttattacgcg cctgacttca
     6361 agggtcgcga tgaaatacgt ggattaatcg ttctgtaata tttgattgtc tgtgccggat
     6421 gcggcgtgaa tgccttatcc ggccaataaa atcctaaaaa ttcaataagt tgatgttctt
     6481 tcatgctctt ataaaggtcg tgcctctggc ggatgtacgt ttgtcatgag tctcactctg
     6541 ttgctaattg ccgttcgctc ctgaacatcc actcgatctt cgccttcttc cggtttattg
     6601 tgttttaacc acctgcccgt aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc
     6661 tacgctggcg acccgattct tacgcttatg gagcgtttta aagaagaccc tcgcagcgac
     6721 aaagtgaatt taagtatcgg tctgtactac aacgaagacg gaattattcc acaactgcaa
     6781 gccgtggcgg aggcggaagc gcgcctgaat gcgcagcctc atggcgcttc gctttattta
     6841 ccgatggaag ggcttaacag ctatcgccat gccattgcgc cgctgctgtt tggtgcggac
     6901 catccggtac tgaaacaaca gcgcgtagca accattcaaa cccttggcgg ctccggggca
     6961 ttgaaagtgg gcgcggattt cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc
     7021 gatcctacct gggaaaacca cgtagcaata ttcgccgggg ctggattcga agtgagtact
     7081 tacccctggt atgacgaagc gactaacggc gtgcgcttta atgacctgtt ggcgacgctg
     7141 aaaacattac ctgcccgcag tattgtgttg ctgcatccat gttgccacaa cccaacgggt
     7201 gccgatctca ctaatgatca gtgggatgcg gtgattgaaa ttctcaaagt ccgcgagctt
     7261 attccattcc tcgatattgc ctatcaagga tttggtgccg gtatggaaga ggatgcctac
     7321 gctattcgcg ccattgccag cgctggatta cccgctctgg tgagcaattc gttctcgaaa
     7381 attttctccc tttacggcga gcgcgtcggc ggactttctg ttatgtgtga agatgccgaa
     7441 gccgctggcc gcgtactggg gcaattgaaa gcaacagttc gccgcaacta ctccagcccg
     7501 ccgaattttg gtgcgcaggt ggtggctgca gtgctgaatg acgaggcatt gaaagccagc
     7561 tggctggcgg aagtagaaga gatgcgtact cgcattctgg caatgcgtca ggaattggtg
     7621 aaggtattaa gcacagagat gccagaacgc aatttcgatt atctgcttaa tcagcgcggc
     7681 atgttcagtt ataccggttt aagtgccgct caggttgacc gactacgtga agaatttggt
     7741 gtctatctca tcgccagcgg tcgcatgtgt gtcgccgggt taaatacggc aaatgtacaa
     7801 cgtgtggcaa aggcgtttgc tgcggtgatg taatgcagga aagcaggctg gagctaccca
     7861 gcctgcagtg aaattaaact gtcgtcgctt tcactctttc tttatagatg atttttttga
     7921 tgccatcgtt ctacgtgaga gataataaac gttgttagtt cttttattgt taagtttatc
     7981 ccaattatct ggaattcctt atcctgtttt tttgggtgga gtaataattt ctgcacatct
     8041 aaccattatt aaatttaata ataagtatcg ggtttgttac gatagaatat ttctatatcc
     8101 tcaagaatat gctttgcctt attgcctgtg actttctgag attcaattat agtatcgtta
     8161 aattctaatg ttaaagagaa ctcttttttc cgctttgtgt aagtttaacc ttatattgat
     8221 taccaatatc aaataaaaag gtcctgtaat gagaataatc acctttaacc tcctcggcaa
     8281 aacagcaaag cgtgcaaata tacataaagt ctttcttacc cctgattttg ttaactaaat
     8341 caataaatgc gtgaaaaact ttacttgcaa tagaacttga tacttcttct ccaggtctaa
     8401 aaaatctttt gcaattttta actgtgtttc tatgtataaa aagaaatctt tggcataaga
     8461 gatatcttta ttatttagtg catctagctc aacaggtaaa gatgtcgttt tgttgctatt
     8521 cacatatgat atattcatat catcatctcc atcgcgagta ataaaattaa tcaccattgt
     8581 agggtagggg gctggtcaat cagaaatcat ctttataaac ttcgattgtt tttgtaatgc
     8641 tgtatcatta agttcattaa atcgtacagc agataaatgt tctatcaaat ttcgctcatt
     8701 tgccgaggat tcatcataat aaacgtaaaa ttaatgtatc cttacatcga gtaataaaca
     8761 ttttttatac aaaaaaagac aggaacgtat ttactgggtt aaatataatc atcctgcttt
     8821 tcatcacaaa aaccgcagat aatccttcct ttccccggca gctggcgtta tggtcagatg
     8881 gtttttgcaa caaatctcac aataaaaagt ttcaacatac tgactattta gggaaaaata
     8941 tgcgcaagat cacacaggca atcagtgccg tttgcttatt gttcgctcta aacagttccg
     9001 ctgttgccct ggcctcatct ccttcaccgc ttaaccctgg gactaacgtt gccaggcttg
     9061 ctgaacaggc acccattcat tgggtttcgg tcgcacaaat tgaaaatagc ctcgcagggc
     9121 gtccgccaat ggcggtgggg gttgatatcg atgacacggt acttttttcc agtccgggct
     9181 tctggcgcgg caaaaaaacc ttctcgccag aaagcgaaga ttatctgaaa aatcctgtgt
     9241 tctgggaaaa aatgaacaat ggctgggatg aattcagcat tccaaaagag gtcgctcgcc
     9301 agctgattga tatgcatgta cgccgcggtg acgcgatctt ctttgtgact ggtcgtagcc
     9361 cgacgaaaac agaaacggtt tcaaaaacgc tggcggataa ttttcatatt cctgccacca
     9421 acatgaatcc ggtgatcttt gcgggcgata aaccagggca aaatacaaaa tcgcaatggc
     9481 tgcaggataa aaatatccga attttttatg gcgattctga taatgatatt accgccgcac
     9541 gcgatgtcgg cgctcgtggt atccgcattc tgcgcgcctc caactctacc tacaaaccct
     9601 tgccacaagc gggtgcgttt ggtgaagagg tgatcgtcaa ttcagaatac tgacagagcg
     9661 ggagagcgtg atgctctccc gaatgctgtt tttttaatca cacctttatc ctttcgctgt
     9721 cttgctgcaa actgattaag agagttttat caaggagcag cacatgtggt atcaaaagac
     9781 gctcacgctt agcgccaaat ctcgtgggtt tcatctggta acggatgaaa ttctgaatca
     9841 gctggctgat atgccgcgcg ttaacatcgg cttactgcat ctgttgctgc aacatacctc
     9901 cgcctctctg acacttaatg agaactgcga tcccaccgta cgccacgaca tggagcgttt
     9961 tttcctccgc accgttcccg acaacggaaa ttatgagcat gactatgagg gagcagacga
    10021 tatgccttct catatcaaat cctcaatgct gggaacatcg cttgtattgc cggtgcataa
    10081 agggcgtatt cagaccggca cctggcaagg catttggctg ggggaacatc gcatccacgg
    10141 cggatcgcgt cgcatcatcg cgacactaca aggggagtaa aaaatgacca tttcggagtt
    10201 gctacaatat tgcatggcaa aaccaggcgc agaacagagc gtgcataacg actggaaagc
    10261 gacgcagatc aaagtggaag atgtactgtt tgcgatggtg aaagaagtag aaaatcgccc
    10321 agctgtttcg ctgaaaacca gcccggagct ggcggagctg ctacgtcagc agcacagcga
    10381 tgtgcgtcca agccgccatc tgaataaagc gcactggagc accgtgtatc tcgacggttc
    10441 gctgccagat tcgcaaatct attatctggt ggatgcgtct tatcagcagg cggtgaattt
    10501 actgccggaa gaaaaacgta aattgctggt gcaactctga aaggaaaagg ccgctcagaa
    10561 agcggcctta acgattacag catcggctta aggaagcgtg ccgtgtgtga tgcttcgcac
    10621 tccgcgacgg tttctggcgt accggagacg aggatctcgc cgccaccact gccgccttct
    10681 ggtcccaggt cgacaatcca gtcagcggtt ttgatcacgt cgagattgtg ctcaatcacc
    10741 acaatggtgt tgccctgatc gcgcagttta tgcagtacgt cgagcagttg ctgaatatcg
    10801 gcgaagtgca gaccggtggt cggctcgtcg agaatataca gcgtctgccc ggtgccgcgt
    10861 tttgacagtt cacgcgccag cttcacgcgc tgggcttcac cgcctgaaag ggtggttgcg
    10921 gactgcccca gtcgaatgta cgtcaggcca acgtccatca acgtttgcag cttacgcgcc
    10981 agtgcaggta cggcatcaaa gaactcacgc gcctcttcga tggtcatatc cagcacttcg
    11041 tggatggttt tgcctttgta cttaatctcc agcgtttcac ggttatagcg tttacctttg
    11101 cactggtcgc acggcacgta gatatccggc aggaagtgca tctccacttt gatcacgcca
    11161 tcgccctgac aggcctcgca gcgtccgcca cgaacgttaa agctgaaacg tcccggcgta
    11221 tagccgcgcg cacgggattc cggtacgccc gcaaacagtt cgcgcacagg cgtaaacacg
    11281 ccggtatagg tcgccgggtt agaacgcgga gtacgaccaa ttgggctttg gtcgatatcg
    11341 atcactttgt cgaaatgctc cagtccctga atatcgcgat acggtgccgg ttcggcgatg
    11401 gtcgccccat tcaactggcg ttgggcaatc gggaacagtg tgtcgttaat cagcgtcgat
    11461 ttaccggaac ctgaaacccc ggtgatgcag gtaaacagac ccaccggcag cgtcagcgtc
    11521 acgtccttca ggttgttgcc gcgtgcgcct gtcagcttca gcactttttc cggattcgcc
    11581 ggaacgcgtt tcttcggcac ttcaatcttg cgtttgccgc tcatgtactg cccggtcaac
    11641 gactccggca ccgccataat cgcttccagc ggaccttctg cgaccacttc accgccgtga
    11701 acacctgcgc ccgggccaat gtcgatcaca tggtcagcgg cgcgaattgc gtcttcgtcg
    11761 tgctccacca caatcacggt attaccgaga tcgcgcagat ggataagcgt acccaacagg
    11821 cgctcgttat cacgctggtg caggccgata gacggctcgt ccagcacgta cataacgcca
    11881 accaggcccg caccaatctg gctcgccaga cggatacgct gtgcttcacc gccagaaagc
    11941 gtttctgccg agcgggaaag cgtcaggtaa ttcaggccga cgttaacgag gaatttcaga
    12001 cgatcgccga tctctttaag gattttttct gcaatcttcg cccgctgacc tgcgagtttg
    12061 agattgttga agaattccat cgcatgacca atgctcatgt cggagatagc aggcagcggc
    12121 gtattctcga catacacgtg gcgcgcttcc cgacgcagac gcgtcccttc gcagctggcg
    12181 cacggacgat tactgataaa cttggctaat tcttcgcgta ccgcgctgga ttccgtctct
    12241 ttatagcggc gctccatatt atgcagcacg ccttcgaacg gatgacgacg aatggaggta
    12301 tcgccacgat cgttcatgta tttgaattca atgttttctt tgccagaacc gtacaacacc
    12361 actttatgca cgttcgcgct caggctgccc cacggcgctt cgacgtcgaa cttatagtga
    12421 tctgccagcg atttcagcat ctggaaataa tagaagttgc ggcgatccca gccacggatc
    12481 gcaccaccag ccagcgacag ttccggattc tggatcactc gatcaggatc gaaatattgc
    12541 tgtacgccaa ggccgtcgca ggtcgggcag gcccccgccg ggttgttaaa cgaaaacagt
    12601 cgcggctcca gttcacgcat actgtagccg caaattgggc aggcgaagtt ggcggagaac
    12661 agcagctctt ccgctttcgg gtcgtccata tccgccacta ccgcggtacc accggaaagc
    12721 tccagcgcgg tttcaaatga ctcggcaaga cgttgggtaa gatcgtcacg caccttgaag
    12781 cgatcaacca ccacttcaat ggtatgtttc ttttgcagtt ccagttttgg cggatcggaa
    12841 agatcgcaga cttcgccatc aatacgagca cggatgtagc cctggcttgc caggttctcc
    12901 agcgttttgg tgtgttcgcc tttgcgctct ttaatgattg gcgcgagtag catcagacgc
    12961 ttgccttccg gctgcgacag cacgttatcc accatctggc tgacggtttg cgccgccagc
    13021 gggacgtcgt ggtccggaca gcgcggctcg ccaacgcggg cgaacaacaa acgcaaatag
    13081 tcgtggattt cggtgattgt ccccaccgta gaacgcgggt tatgagacgt cgatttctgc
    13141 tcaattgaga tggcaggaga aagcccctca atatgatcga cgtccggctt ttccatcagt
    13201 gacagaaact gccgcgcgta ggcggaaagg gattcaacgt aacggcgctg cccttcggca
    13261 tataaggtgt cgaaagcgag cgaggatttg ccagaacccg aaagcccggt cacgacaatg
    13321 agcttgtcgc gggggataac gaggttgatg tttttgagat tatgggtgcg ggcgccccga
    13381 acttcgatct tatccattca cctttcccgg attaaacgct tttttgcccg gtggcatggt
    13441 gctaccggcg atcacaaacg gttaattatg acacaaattg acctgaatga atatacagta
    13501 ttggaatgca ttacccggag tgttgtgtaa caatgtctgg ccaggtttgt ttcccggaac
    13561 cgaggtcaca acatagtaaa agcgctattg gtaatggtac aatcgcgcgt ttacacttat
    13621 tcagaacgat ttttttcagg agacacgaac atggccagca gaggcgtaaa caaggttatt
    13681 ctcgttggta atctgggtca ggacccggaa gtacgctaca tgccaaatgg tggcgcagtt
    13741 gccaacatta cgctggctac ttccgaatcc tggcgtgata aagcgaccgg cgagatgaaa
    13801 gaacagactg aatggcaccg cgttgtgctg ttcggcaaac tggcagaagt ggcgagcgaa
    13861 tatctgcgta aaggttctca ggtttatatc gaaggtcagc tgcgtacccg taaatggacc
    13921 gatcaatccg gtcaggatcg ctacaccaca gaagtcgtgg tgaacgttgg cggcaccatg
    13981 cagatgctgg gtggtcgtca gggtggtggc gctccggcag gtggcaatat cggtggtggt
    14041 cagccgcagg gcggttgggg tcagcctcag cagccgcagg gtggcaatca gttcagcggc
    14101 ggcgcgcagt ctcgcccgca gcagtccgct ccggcagcgc cgtctaacga gccgccgatg
    14161 gactttgatg atgacattcc gttctgattt gtcattaaaa caataggtta tattgtttta
    14221 aggtggatga ttaaagcatc tgccagccat aaaaaagaag cctccgttat ggaggcttct
    14281 acgtatcagg tcaaaatcat tggccattgt ggcggtgtct gattcatctc ttctatcatc
    14341 accgactcga tgttgtgcca gatagcggtg atgtccgcca ttgtgatgcc aagcaacccc
    14401 attgcgaacc aacaggcgga aacaacgccc agaccgctgc tgatcaccgc aagcccgaca
    14461 taatcagacc gacgaaaacg gatgttgagt gtgctggcca gaaacatcat tacggcacta
    14521 agaagttgcc agcgaagaag aaccacgcca gtggtgaggg tagccatcaa acaattcctc
    14581 tgaaaagagc cgatgccctg gacagcgcgg cgttgttcac gggaggcgtg gtacactctg
    14641 gctatcgcgg ggcttgcaga acacaaaaat gaaacactct gtttgtttca ttaattttgt
    14701 gaactatatc acaattgatt gtttgttagc catattaggc cgtgactttt attgctgtac
    14761 agattatgtg gtttttcagt ggcattaagg gcatatcttc ccgccgcctc tgcattcctg
    14821 taggaaatta attttgaata tcaatgaatt attttcatcc aggtgacgat tagaaaggta
    14881 tcaatttcaa atcaggcaaa agtgctattt ataccgtaag atttatctaa agacgtcggt
    14941 acccagggtt ttcaccttgc aatggccggg tataaacagg caggaaattg atagcaatga
    15001 gtcatcgtgc acgacaccaa ttactggcgt tgccgggcat tatcttttta gttctctttc
    15061 ccatcattct ttcgctatgg attgccttcc tttgggcaaa atcagaagtg aataatcagc
    15121 tccgaacctt tgctcaactg gcactggata aatccgagct ggtcattcgc caggcagatt
    15181 tagtgagcga tgcagctgaa cgctatcagg ggcaagtttg cactccagcc catcaaaagc
    15241 gaatgttgaa tattattcgt ggctatcttt atattaatga attgatctat gcccgtgata
    15301 accatttttt atgctcatcg ctgatagcgc ctgtaaacgg ctatacgatt gcaccggccg
    15361 attataagcg tgaacctaac gtttctatct attattaccg cgatacgcct tttttctctg
    15421 gctataaaat gacctatatg cagcggggaa attatgtggc agttatcaac cctctcttct
    15481 ggagtgaagt gatgtctgat gatccgacat tgcaatgggg tgtgtatgat acggtgacga
    15541 aaaccttttt ctcgttaagc aaagaggcct cggcagcaac gttttctccg ctgattcatt
    15601 tgaaggattt aaccgtacaa agaaatggct atttatatgc gacagtttat tcgacaaaac
    15661 gcccaattgc agccattgtt gcgacttcat atcaacgtct tataacccat ttttataatc
    15721 atcttatttt tgcgttgccc gccggtattt tggggagtct tgttctgcta ttactctggc
    15781 tacgtattcg acaaaactat ttatctccca aacgtaaatt gcaacgcgcc ctcgaaaaac
    15841 atcaactttg tctttattac cagccaataa tcgatatcaa aacagaaaaa tgtatcggcg
    15901 ctgaagcgtt gttacgttgg cttggtgagc aggggcaaat aatgaatccg gcagagttta
    15961 ttccgctggc agaaaaggag gggatgatag aacagataac tgattatgtt attgataatg
    16021 tcttccgtga tctgggcgcg tacttggcaa cacatgccga tcgctatgtt tctattaacc
    16081 tgtcggcctc cgattttcat acgtcacggt tgatagcgcg aatcaatcag aaaacagagc
    16141 aatacgcggt gcgtccgcag caaattaaat ttgaagtgac tgagcatgca tttcttgatg
    16201 tcgaaaaaat gacgccgatt attctggctt tccgccaggc tggttacgaa gtggcaattg
    16261 atgattttgg tattggctac tctaacttgc ataaccttaa atcattgaat gtcgatattt
    16321 tgaaaatcga taaatcgttt gttgaaacgc tgaccaccca caaaaccagt catttgattg
    16381 cggaacacat catcgagctg gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg
    16441 aaactgagga gcaggttaac tggctgcgca aacgcggcgt gcgctattgc cagggatggt
    16501 tttttgcgaa ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc
    16561 gggagttaac gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg ggagtgcgat
    16621 caaactgccg acggaaaacg cgggagaagg tctgctgcga gacataaccc aggtccattg
    16681 cgatatcaaa aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacaggcgac
    16741 gttggcgaat gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatt cgttgcaagt
    16801 accactttga atagcctgat ttttttgcga ctacatcaat gttaagcggc tggtcaatat
    16861 gctcgtcaat ccatgcgata agatcctgaa taattttctg atgggacata aatctgcctc
    16921 ttttcagtgt tcagttcgtt aattcatctg ttggggagta taattcctca agttaacttg
    16981 aggtaaagcg atttatggaa aagaaattac cccgcattaa agcgctgcta acccccggcg
    17041 aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa agtaaagggt
    17101 tgattaccag tatccgtaac agcggcaatc agcggcgata taaacgtgat gtgttgcgat
    17161 atgttgcaat tatcaaaatt gctcagcgta ttggcattcc gctggcgacc attggtgaag
    17221 cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa cagctttcgt
    17281 cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg cgtgacgaac
    17341 tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg
    17401 gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa
    17461 gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta tcccgctggt
    17521 acacaggagg gtttcccccg acgtcaacac acctcattcg agcacgtggt ggaggttccg
    17581 gttggtgttg atgctttaat tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac
    17641 tttttaaccg caatattttt gtcatctcag acgatttttt atcgcaatcc tgaacggtat
    17701 acggctcgat aacgctgcaa tcttgcgcac cgacgataac gtttgcgcat caattgcctg
    17761 gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt gaaaaagccg
    17821 ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc gcggagctaa
    17881 acgtttgctt ttttgcgacg cagcaaattg tcgcaaacct ggagcaggaa gataacgttt
    17941 cgctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca ggggatgttt
    18001 tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc
    18061 acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat
    18121 ggtctactcg gtcatcgtcg ttccaggtat gttgggtaaa gcaggcttcc cgcctgcggc
    18181 agttttcgtt gcaacctgtc tggttgccgg actcggttct atcgtgatgg gtctgtgggc
    18241 taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg cattcagcct
    18301 ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt
    18361 gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca acttgcctca
    18421 cggtgtggcg cacggcacgg ggattggtat cggtctgttc ctgctgctca ttgccgctaa
    18481 cggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc tgggtgattt
    18541 cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa
    18601 actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg tcggtttgat
    18661 cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga gcgatgaaaa
    18721 cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc
    18781 aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta tccgtgccgt
    18841 cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggtg ggaaagcact
    18901 gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgcggctc cggcagcggt
    18961 atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga cggctatcac
    19021 cgttggcgtg ctgttcctcc tgattctgtt cctctctccg ctctcttacc tcgttccggg
    19081 gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca acgtggcgaa
    19141 aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggctg tattcatcgt
    19201 gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg tgattggtcg
    19261 tctggtttcc ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct
    19321 ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct gaaaacgggt
    19381 ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc gatattctga
    19441 aaaaatgaga attcaggcat aacgtctttc tcagatcata aaaaacatcg caaacaggga
    19501 acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcgc tgtccggggt
    19561 ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg ccatcggtgc
    19621 gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac tctttttagt
    19681 cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc gtgaatttct
    19741 cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca ccgtggtcgg
    19801 cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccctg cctttgcgct
    19861 ggcggcggtg ctttctccga ccgatgctgt ggcgctctcc gggattgttg gcgaagggcg
    19921 catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg acgcctccgg
    19981 tctggtgtcg ttgaagtttg ccgtagcagt ggcgatgggg acgatgatct tcaccgtcgg
    20041 cggtgcgacg gtcgaattta tgaaagtagc cattggcggt attctcgccg gttttgtggt
    20101 gagctggctg tacggtcgct cgctgcgatt cctcagccgc tggggcggtg atgaacccgc
    20161 gacgcagatc gtcctgctgt tcctgctgcc attcgcttct tatctgattg ccgaacatat
    20221 tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc gctccggtgt
    20281 gatgcgccgt gcgccgctgg caatgcgcct gcgtgcaaac agcacctggg cgatgctgga
    20341 atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg gtattctgga
    20401 gacgtcgctg atggcagcag aaatcgaccc taacgtcgaa atctggatgc tgtttaccga
    20461 tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt ggacgatgaa
    20521 aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt ggaccacacg
    20581 agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg ccggtgtgct
    20641 ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg agctggtgtt
    20701 cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt tgcctattct
    20761 gctacaacac attgaagtcg cggatcattc gcaacaattg aaagaggaac gtattgcgcg
    20821 agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc gtctggcggc
    20881 ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc gcgtcattgg
    20941 taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc aggaagagaa
    21001 ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac tttaccacct
    21061 gcgcgccaca cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg atctcgattt
    21121 gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa ccaggcccga
    21181 ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg gctaacattc
    21241 atgattctaa aacaaataag ttatctctct tttaaagtca attcattggc tgttcttcga
    21301 cttaacctca atctgcatct tgcataatat ctgcagtaat cttcatagcg actgcatgaa
    21361 gattatcctc agtttgtgac atgccagtat gagtggcaca atcttcttca aagaatttct
    21421 gtagtgttac caggatatca ctattaatgg agatattttc ctcaccacgg agaatggtaa
    21481 tacccattcc ctggacgcta gcctcatcga aattaacacg atagagacgg atattaaaca
    21541 tccaggcttt tgttaaaaca gaacagttga aatggcaatt gcttaaattg gcattattaa
    21601 aattaacatt agccatatta gagccggtac atataaccat ttcgaggaac gtatttgtta
    21661 accgcgtaaa aaagagtttg ctatcaatta agtttatatt ctggagggtt gctttattca
    21721 ggttgacacg agacagatca gcatgttcaa agttaattaa atcaagttta gagaagctaa
    21781 ggtcggctct aaataaatta atatatggc
//
LOCUS       DAZFZC010000087        19625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFZC010000087 DAZFZC010000000
VERSION     DAZFZC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.088"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..3206
                     /gene="dpdE"
                     /locus_tag="ACHKXP_003718"
     CDS             150..3206
                     /gene="dpdE"
                     /locus_tag="ACHKXP_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdE"
                     /protein_id="HFO7648646.1"
                     /translation="MTTLPNTFSEGDLVTSLLFGGIAKIVRLHPPGKAEIAWFVSPLE
                     YEVNRQMAETVSLTPAKLYEESTVFFRSPESGLWCRGRYGGQRPDNKHLVIVRSDKNV
                     VIDLADIFYPNYGQGLAMNPAHFLAARANDAPFFYPLREKFVSAWIKQRAACRSMSSL
                     ISSRVEIEPHQIAIVRRILQDQNPKYLLADEVGLGKTIEAGLVIREHILECKREARVL
                     VVVPQVLQGQWTQELVDRFALQEVMSGGRHTQIRLCLPEELCQPELIGWKPTMVVIDE
                     AHQLTRQAWADDEDDLAQYAAMAALCHQARIVLLLSGTPMHGNERHFLAMLHCINPQA
                     WQLNAQGIEKFMQRVAERERLGGIYSSLTPETPNGVLEEILEELRLLFADDEQLQARI
                     VGLSPLVDFFAPDESEERTAGILALRQWIGERYRLFHRLLRNRREDPALEYLFPGLAG
                     LEKCVWPVASTEITLDETLDAWRAAAWRNPAQHQHLDSTTLDEWVEALFLNPLVMSRQ
                     AKKALVKGTASEEEFNFLEQIIETSQQEQKAKDVALMKALTDWFEKCPDGKAVIFCGE
                     GSEAAFLFTKLQIQAPWAVVRHAPDQRKQSELLDDSWQVLICDRRGEDGLNLHGNNRL
                     AVHYSLSRDFNRFEQRLGRFNRYSGNLRGVKPVKSLVLLPERDGLRADWVKLLDEGTG
                     LFHRSVASLQFVLSEQLDVVWRDYVGQGLAVFHEAQQRFSGENGFIAQERKRVLAQEN
                     LLSMEQEVIAAREFSEQLAESEDDAEEQAKDMLAWMCKALGFKREKYPEGGFRLRFER
                     GEYQRQTLVDVGTFIDNCLLGLDFSEGYPPSTAMMSLSRTEAGNHKHVYPLRYGQPFV
                     ETVWQLMQSDPRGASMALLRVLSSAVALKQPHTWFHFQWLSEAQVEGENQLAAQRRGD
                     ECFSPVVHNFWLDDGGKEADPQIVVSLLDKPYDEEGNRLFQDINLREEVWTRMPDWFD
                     PHSWKETVLAAAEQARQNVHAHYGDRPVRHQLLAMKAIILCTRDML"
     gene            3219..5669
                     /gene="dpdF"
                     /locus_tag="ACHKXP_003719"
     CDS             3219..5669
                     /gene="dpdF"
                     /locus_tag="ACHKXP_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdF"
                     /protein_id="HFO7648647.1"
                     /translation="MENQWQQLRAVLNGELNERDGLSGQFFNRLLYCLDDVRAGECDR
                     LHAFYDALQCARALGLTPLRLPYRHLTEGTMLQHFGLRRDHDYPGELLLTREAEEIDP
                     HLLPVWKGEQRRFLETPPLDVLLPSVLNDPRYTHYTSAGQQQAVRTVLTCGRDKTLLV
                     NLPTGSGKTFVIHSQMLTSPRRHLTLVIVPTVALAIEQALRAQEVMQQAGQDHGGRYV
                     WHSGQSTEERNEMKSRLASGEQRALFCSPEAATGGLLLQLFSLAERGLLGAVIVDEAH
                     LIDQWGAEFRPEFQLLAPLVRSLKATSPDGVKVVLLSATFSQSTLNTLKALFAGKRQG
                     SVIEVNGSFLRPEPVWYVSEAASHEDHLAQVENAIARLPAPMILYTTEVEQAKFWYQY
                     LRERGYRRCGLFHGATPMQEREGLIHAWRNDGLDIMVATSAFGVGMDKNNVRSVLHVA
                     VPENLDRFYQESGRGGRDGKASVAHIIFHHKQLQVARNINRTKLIGASKGYLRWAKMH
                     QLREQHQPGQFIVPLRAKHADIRMDSQSNVDWNLRTLLLMQRAGFIDIAYPPPDLSAI
                     APDERDEAKIRAWFDHYFNHIQVSVRRDGHMDEARWHEGIQAHRSHELGMRKQGYSAL
                     EEWLNDPSISLCQKLAQFYTLDGFVPEIACGGCPACRSKGYPPFTPTLGRIAHVTGEA
                     VRNVMGNEQRVYYPATLTNRLLLRQWSDWIARLLANRQIQAIRASQSVLAQLSAVLPA
                     GLPFWCSLAVDEENTCWDELVLVLPDEAMPELDIFATINRIIVAPERLQEPGYRGRRW
                     WEVDTSAVALEQFQRNII"
     gene            5669..6568
                     /gene="dpdG"
                     /locus_tag="ACHKXP_003720"
     CDS             5669..6568
                     /gene="dpdG"
                     /locus_tag="ACHKXP_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdG"
                     /protein_id="HFO7648648.1"
                     /translation="MAITNNAAAGSQIRLLCMIDRVLNRRTGQAIPMVDLIELLRPEM
                     LPVSDGARKRLPAEVSFWTKEGLWKNESAGLSQQSPLSSERNLPARVLRTLINSEKTT
                     PLLTGTRGQPLLMCVTSMLAQEKYTLRGSTPLTKEAVPLAVGPMINNKLAGEGRRNLN
                     VSNEAESFLNYAYFLGFTEPYLNGWVMDPTRAIEGILDNLGLTAGMPIQEFLSQLAEF
                     LPMLDGGAYRQQVEPMISADNWQPLEKHMISASLSQALLRLELTMQLVFSSRSDDLDA
                     MVLQGPDGSLRRISTVSPGEARK"
     gene            6571..9714
                     /gene="dpdH"
                     /locus_tag="ACHKXP_003721"
     CDS             6571..9714
                     /gene="dpdH"
                     /locus_tag="ACHKXP_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdH"
                     /protein_id="HFO7648649.1"
                     /translation="MIEYWPSLKNINECIRTEAEELAEYTLLAVHQPVNLLRRDKDGN
                     NLGYAKEEDLLNHFLETPRPIPVVGKAGVGKSHIIRWLDAKLRLRQEYKDKKWHIVRI
                     PKSASLREVLTSMLAGLEGEIFDEAREDINKVSDKRTPREIAEWLLMLMGQELRNLHE
                     RNKQDMEALKKQMAGSTPEQLKAMKPQATRLQKIHIHAEDNALPTLINDAYFKQFLLK
                     EEHCLYRLASRLTNGATSSDLDEGEQQLQAKDLDFSFNINDLSLPARQYIQKSRLNTH
                     EDGRKDASEILNLVLGKATQALFNQLFNFRGRSFSDLFTQIRQALHEKHMTLMVLVED
                     MSLITAIEDVLIDSLEREGIRDGKEVLCPVCSAIAVTDGYQGYARRRQGMLDRAKGEW
                     VIEEVGSGREETRLRIVDFCGRYINAARFGSEALLQRWEQAADKSHWPPDWEASANGD
                     EMAEVFGRSEQGFSLYPFNASAIHALAEAFCRDDRNELKFNPRQIINQILLRVLQHCR
                     RDADEGRFPPPRLGDIAAPAGLRSWLFRQGFADTERAESVVALWGYPADSDATLASTL
                     PPDVARSFDLEDLAQVLENTKSAPLVEQSVMPTRVTKVEQQVKKTVQPPKPVKPVLKE
                     DKDTLAIRALDAAVGDWMLKGAPLEIDPARYIKSSLAFFFDKRAVAEWAGSSYRPTLW
                     LGKSNFVAVELPNAQGNRGVHVVKFISQSEYDKRSVQLTDAAMALARFGYYRENSLTK
                     TEDWSYPEGKTDYLIIQSFCDRWVNYALTELVKHKRNDLPLLLSEQIALADALGVIKT
                     ADGPKEVLGRLLQNSKTLSSQFRTGITKAITELRGEALAKWDDAQDAWLSLVALNDHA
                     LEGDLLLSAIQKVLKKRSNNTHAAVVKKSLSEIRPALDTAALFADCENADDFSELVTG
                     LTQLVKSLGDSGDYPADMTPDSSTLANSLTGLTEGGVWLTILKLRGITQSEDPLRQWQ
                     LLCELDGTLINRLIFTISGWQQVNKRVLANITAYNHSHGSHQISEFRTQIESTLQELH
                     QVLDAMQSVAGEQYDNA"
     gene            9701..10435
                     /gene="dpdI"
                     /locus_tag="ACHKXP_003722"
     CDS             9701..10435
                     /gene="dpdI"
                     /locus_tag="ACHKXP_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdI"
                     /protein_id="HFO7648650.1"
                     /translation="MTTLKTQLTQLREQYQIKQQVQSLEEVYDRYRTIRDGLLDVAEP
                     LQKAQKQLEVIGTLPEPHSAIDFSQEAALFGGAKTQLDALTARFKESGDKTEQCGFWE
                     TVRVLKSVHESIDDKVDATWKAFVADLEARATLDPVFLEQQRTLGNVLVYDDYRKARD
                     NFNRQKLSGPDSLSVVKNLQKYSDEMIALKRSMDLDAPDDVLRFFEALDRHNHASLTL
                     LTPGVITWLEEKDMLPAFNVTRKRMI"
     gene            10435..14949
                     /gene="dpdJ"
                     /locus_tag="ACHKXP_003723"
     CDS             10435..14949
                     /gene="dpdJ"
                     /locus_tag="ACHKXP_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdJ"
                     /protein_id="HFO7648651.1"
                     /translation="MKGIDLSLLQRVLDALEEQENALLVWGDTGGFFNLDELLSVIGR
                     VLPDEDAEVVLDQMLEAAMLIQVPHVSGTPVWRTRMGETVHLMRNLRQWMHKQKLDQS
                     KTLVSDYRFIRRPRSYPDRVYEPATLISGWKKQLKLSDRVCDIMRQALASDKPFLLAG
                     FQVRATERIMQCWQDHQVKSNTASGTIVCAGTGSGKTLSFYLPALTRLAEDICNNPER
                     KVRILAIYPRKELLKDQFAETFSQCRKLDDYMLTAAGRKIRIGAFFGDTPVKAEWSKE
                     DVKGKVGLPFGLMKCQQPHPHHPKQRCGGALIWRREDVFGEREVLTCTQCQHQVDQSE
                     IMITRDAQQHRGDAPDILFTTTEMLNLQMNSTWSNHLFGVGEGYGPTLVLLDEVHTYS
                     GTTGAQTAFLLRRWMQRTDCLPHFVGLSATLTDAQHFFARLVGAAEEQVALIQPYMED
                     MIEEGAEYLLALRGDPVSETALLSTTIQASMLMARMLDSKANKSQGTWGKKTFIFTDT
                     LDGNNRLYHDLSDAEGWETGPGHSRIDHSPLAVLRSPFDNTAPERSKTELGQNWRAAT
                     DIGHDLAENKVISRTSSQDAGVDASADVVVATSSLEVGYNDPLVGAVLQHKAPNDVAS
                     YLQRKGRAGRPRGMRPWMLVVLSEFGRDRVEFQRYEGLMSPEIKRQGLPLDNQHVQKM
                     QAAMATLDWISKVGQFKDLWSMLKKAEHNQLKYNRMYGPLIKLIEEVLSGGRRLNELM
                     RYLQDALQLSDGAVQNILWSPPRSIMFEFLPTILRNLRTRWSVNGVEWAGLRPNQPNS
                     EGEQHRSNSPVPEYIPQNLFSELNLPELDIRLKRGFDDEDHWETLSFWQGIREFAPGR
                     LSKRYAVKSNKSTDWLVPQSYEPMAGEGRQFVDFQISDAFGDSWQNEYEVDYMGKTIK
                     VVKPSKVMTTRADIRRINDKSNAQLQWVFNVINPAIATPDEVPKGPWKHTLSDVTFYN
                     HQHMTPLELVRFSTGSQASLRFRNKERAHVDFTWVNGEEQVGVGSRQWVDAMRLRFNL
                     TCDDVLGLLHQEEIQRGMRPVYFQHLVRQSPEFEFDSFNADWAIECFMAQLAETLANG
                     AHASVESALREMASEKGGERLADIPASLFQPDTDNETGTDQALQIGLNKLLQRPEIQQ
                     LLLNCAQALWKPLDEIDGFVEWARQVLADTLAAGVQQTLSTLLPDVDELAVVTDSSWM
                     SDPRKGAEWLEIWLCEMESGGSGILIRLQQKWAEDPVSFLNVLVRNLSASDYEQIDYD
                     LRTVLQMMQTDDTLRMAISAVREASNMDARREANKNLHLQLSQRGMRLSHSFTTVLYS
                     RILRAGSGEHTDTQLYQLLSDWLSLEASAGIEFSMNTMAHALAVNSLGVETDASVVFD
                     EQCRNQNLLWPRGYTIRQAELGFYNMFCSRKVTTERLLAGALFSEQIEKIALDDDWLG
                     HLHAALRKGGRAELILTRQQRNQLHQVITTVQIEPVDHLGLLLYPRLGEVRREQDVLI
                     LRIELAEAMQ"
     gene            14946..15491
                     /gene="dpdK"
                     /locus_tag="ACHKXP_003724"
     CDS             14946..15491
                     /gene="dpdK"
                     /locus_tag="ACHKXP_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein
                     DpdK"
                     /protein_id="HFO7648652.1"
                     /translation="MIGRAKQRQIFLHGSAGHRQLREVLSAQVVALIMQPEPIWLVSP
                     WVSNFSLLDNRSGNWDSIDPAWGGREVDFIELLACAVNNGAPLSLVTRDEPMNRIFVK
                     ALEKRLSNFATFRLEWRNQLHTKGFLTPNFFLKGSMNFTRSGVNRNEEFVELNCDSHM
                     IGEAMAEFESQYLFIGSKEAF"
     gene            15491..17662
                     /gene="dpdD"
                     /locus_tag="ACHKXP_003725"
     CDS             15491..17662
                     /gene="dpdD"
                     /locus_tag="ACHKXP_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdD"
                     /protein_id="HFO7648653.1"
                     /translation="MCADWLKNYYAEDNYLDYDKAMQGGYGIPQMNTLIQQAAALRMP
                     CIVPSTRTGRTVFYALAENAKSLDELRRILTAALGSADTTPDIKSLNHSDDGGEQLLL
                     EKSPDGILAFDFLPVPDGSPQQVKEWQAARMKRVYAMLQTVMDLYHQRPVLHSLVSRQ
                     TGRILRDFYTACQARDGKIAEQYLEELRGNQTLSSLNLLFLELQGMAASAKWDAILNH
                     PRLEVLLRGRVPERIQRLLLRSSGHLMLNAIRDAHFPLDRREDARRLVLGLLPLYKHK
                     PRFAHQASFLPDWQLWAMGAALLGIDEWQTATPLLAPDWIQQVEGWATGTTSLPAPVA
                     AEEQALIQAPVIMLISLENATDLLLEALLADAERESEIYAQLAAMPEATRQALQKIPK
                     LWEAWQALKKRCEPQDYGWCRWLADLQQTTESERFESLRQQATVHYMDWAPSTFSETQ
                     WQALLEHQSNTQLSKVLRDVLPTLLNWLEEYDVKVSASLWPDWLMLLAVEDIRSEEDV
                     RLGGMILDKFLSSPFTCEEYSAAIESTEILCAENVSVRTLGYSLDIAELLYDRVTANE
                     ASRLGFWIKLQELLKHRWERLDASMQFSARMVERLYLGEHAGNVFPSEDNTPGVASSL
                     HRDLSGKTLAIYSLMKGAARRGKEALLQLYPGLEVELNHDHVATPALVNLAKTADYFI
                     FASSSSKHQAFYAVTDYRKEIIYPSGKGASSMIASFVRAME"
     gene            complement(17925..18287)
                     /gene="queD"
                     /locus_tag="ACHKXP_003726"
     CDS             complement(17925..18287)
                     /gene="queD"
                     /locus_tag="ACHKXP_003726"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO7648654.1"
                     /translation="MKTTLYKDFTFEAAHRLPNVPNGHKCGRLHGHSFVVRLEITGEV
                     DKHTGWIIDFAELKAIFKPTLDRLDHYYLNDITGLENPTSEVLAEWIWNQIKPQLSIL
                     SAVIVKETCSAGCIYRGE"
     gene            complement(18538..18927)
                     /locus_tag="ACHKXP_003727"
     CDS             complement(18538..18927)
                     /locus_tag="ACHKXP_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7648655.1"
                     /translation="MFAKSFIALDGNDRLTGARTAQTAPYDRYTCHLCHSALKYHPEY
                     DTERPWFEHIDAGSAENGQHNCPYVKPDASEIHLARRLQRFLPDATPRVYKADWNCIQ
                     CGKNYYGECYCSACRTGDFSQLTKMGS"
     gene            complement(19098..19190)
                     /locus_tag="ACHKXP_003728"
                     /pseudo
     CDS             complement(19098..19190)
                     /locus_tag="ACHKXP_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            19204..19625
                     /locus_tag="ACHKXP_003729"
                     /pseudo
     CDS             19204..19625
                     /locus_tag="ACHKXP_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         4736 a         4883 c         5534 g         4472 t
ORIGIN      
        1 cactctgcaa gataatgatg agaaataatt tatctcctga ttacaattgc taaaaaatca
       61 ttcccaccat atattaaatt acatgcatac acagcgatat tgttcgcatc gcaatatgat
      121 gctattcgct acagctaata aaaaattaaa tgactacact acccaataca ttctctgaag
      181 gtgatctagt tacttctctt ttgtttggcg gaatcgccaa aattgtacgt cttcacccgc
      241 ctggcaaggc tgagatcgcc tggtttgtct cacctctcga atacgaggtt aaccgccaga
      301 tggcggagac cgtatcactg acgccggcaa aactgtatga agagagcacg gtattcttcc
      361 gctcacccga gagcggactg tggtgtcggg ggcgttatgg cgggcaacgt ccggataata
      421 agcatctggt gatcgtccgc tctgataaga acgtcgtcat cgatctagct gatatttttt
      481 atccaaacta cggacaggga ctggcaatga acccggccca tttcctcgcc gctcgcgcca
      541 acgacgcacc ttttttctac ccactgcgcg agaagtttgt aagtgcatgg atcaaacagc
      601 gcgccgcctg tcggtcaatg tcgtcgttaa tcagcagtcg ggtggagatt gagcctcatc
      661 agatagcgat tgtccgccgc attctccagg atcagaaccc caaatacctg ctagcggacg
      721 aagtaggact gggcaaaacc atcgaagcgg gactggtcat ccgagagcat attctggagt
      781 gtaaacgtga agcccgcgta ttggtggtgg tgcctcaggt attgcaaggc caatggacgc
      841 aggaactggt cgatcgtttt gcgctacagg aagtgatgag cggaggacga cacacccaga
      901 ttcgtctgtg ccttccggaa gaactttgcc agccggaact gatcggctgg aagcccacga
      961 tggtggtgat cgacgaggcc catcaactga ctcgacaggc atgggccgat gatgaagatg
     1021 acctggcgca gtatgccgct atggccgcgt tgtgccatca ggcccgtatt gtgctgctcc
     1081 tgtctggcac accgatgcac ggtaacgagc gtcacttcct ggcaatgctg cactgcataa
     1141 atccacaggc gtggcagctt aatgcacagg gaatcgaaaa atttatgcaa cgcgtggcag
     1201 agcgcgagcg tctgggggga atttacagct ctctgacccc tgaaacacca aacggggtgc
     1261 tggaagagat cctcgaagag ttacgtctgc tttttgctga tgatgagcaa ctccaggcgc
     1321 gcattgttgg tctgagtccg ttggttgatt tttttgcacc tgatgaaagc gaagagcgaa
     1381 cggcgggcat tttggcatta cgacagtgga taggagagcg ctaccgtctt tttcaccgct
     1441 tgctgcgtaa ccgacgtgaa gatcccgcgc tggaatacct atttccaggt ctggccgggt
     1501 tggaaaaatg tgtctggccc gtcgcatcga ccgagatcac gcttgatgaa acccttgatg
     1561 cctggcgtgc cgcggcctgg cgcaaccccg cacagcatca acatctggat tccacaacgc
     1621 ttgatgaatg ggtggaagcg ctgtttctca acccgctggt gatgtcccgc caagccaaaa
     1681 aggcactggt aaaagggacc gccagcgaag aagagttcaa ttttcttgag cagattattg
     1741 aaacgtccca gcaggagcag aaagccaaag atgtggcgct aatgaaggcg ctgaccgact
     1801 ggtttgaaaa atgtcctgat ggcaaagcag tcatcttttg cggtgaagga tcagaagccg
     1861 ccttcctgtt taccaaactg caaattcagg ccccctgggc ggtagttcgc catgcgccgg
     1921 accagcgtaa gcagagcgag ttgttggatg acagttggca ggtattgatc tgcgataggc
     1981 gcggcgaaga cggactgaat ctgcacggaa acaaccggct tgcggtccac tatagtctga
     2041 gccgcgactt taatcgcttt gaacagcggt tggggcgctt taaccgctat tccggaaatc
     2101 tacgcggcgt taagccagtg aaaagcctag ttttactgcc tgagcgtgat gggctgcgtg
     2161 ctgactgggt gaaattactg gacgaaggca cgggattgtt tcatcgaagc gtcgccagcc
     2221 tgcaatttgt tctgtctgaa caattagacg tggtatggcg cgattacgtt gggcaggggc
     2281 ttgccgtctt ccatgaggcg caacagcgct tttcgggaga aaatggcttc atcgctcagg
     2341 agcgtaagcg cgttctggct caagaaaact tgttatcgat ggaacaggag gtcattgctg
     2401 cccgcgagtt ttctgagcaa ctggccgaat ctgaagatga tgccgaagag caggcaaagg
     2461 atatgctggc ttggatgtgc aaggcattag ggtttaagcg agaaaaatat cctgaaggcg
     2521 gatttcgcct gcgcttcgag cgtggcgaat atcaacggca aacgctggtg gacgtgggaa
     2581 cttttattga taactgcctg ctcggtctgg atttttcaga gggatatcca ccctccacgg
     2641 cgatgatgag cctgtctcgt acggaagccg gcaaccataa acatgtttac cccttgcgct
     2701 acgggcagcc tttcgtcgaa accgtatggc aactgatgca gagcgatccg cgaggtgcca
     2761 gtatggcgct gcttcgggtt ctctcctccg cggttgcgct gaagcaaccc cacacctggt
     2821 tccattttca gtggctgagt gaagcacagg ttgaaggtga aaatcagcta gccgcgcagc
     2881 gtcgtggtga tgagtgcttc agcccagtgg tccacaattt ctggctggat gacggtggaa
     2941 aagaggctga cccgcagatc gtcgtctccc tgctggataa accttacgat gaagagggca
     3001 accgtttgtt ccaggatatc aatctgcgag aagaggtctg gacgcgaatg ccggactggt
     3061 ttgatccgca tagctggaaa gagaccgtac tcgctgcggc ggagcaggcc cggcagaacg
     3121 tgcatgctca ctatggtgat cggcccgtcc gccatcaatt gcttgcaatg aaggcgatta
     3181 ttctgtgtac ccgtgacatg ctgtaatcgg aagcaacaat ggaaaatcag tggcaacaac
     3241 ttcgtgctgt tctgaacgga gaactcaacg aacgcgacgg gctgagtggg cagtttttta
     3301 accgcctgct ttactgcttg gatgatgtac gggcgggaga atgcgaccgc ctccacgcat
     3361 tttatgatgc actgcaatgc gctcgggcac tcgggctgac gccgttaagg ttgccgtacc
     3421 gacaccttac cgaaggcaca atgctccagc attttggttt gagacgggat catgactacc
     3481 caggcgaact gctcctgacg cgcgaagcgg aagagattga cccccatcta ttacccgtct
     3541 ggaaaggcga gcagcgccgt tttctggaaa ccccaccgct ggatgtactt ttgccctctg
     3601 tactcaacga tccgcgttat acgcactaca cgtcggcagg gcaacagcag gcggtacgca
     3661 cggtgttgac ctgcggcagg gataaaacgc tactggtgaa tctgcccacc gggtctggta
     3721 aaacctttgt tattcactcc cagatgttga cttcaccgcg aaggcacctg acgctggtga
     3781 ttgtaccaac tgtggcgctg gcgatagaac aggcgctccg ggcgcaagag gtgatgcaac
     3841 aggctgggca ggatcacggt ggtcgttacg tctggcatag tggccagtcg acagaagaac
     3901 gtaatgaaat gaagtcgcga ctggcatccg gcgagcagcg agcgttgttt tgctcccctg
     3961 aagcagcgac aggtgggctc ttgttacaac tgttctcgct tgcggagcgc ggactactgg
     4021 gcgccgtgat agtggatgaa gcgcacctta tcgatcaatg gggggcggag ttccgtccag
     4081 aatttcagct actggcgcca ctagtgcgat cgttgaaggc tacctcgcca gacggtgtaa
     4141 aagtcgtgct gctttctgcc acttttagcc aaagcacctt gaatactctc aaagcgctct
     4201 ttgcaggtaa acgccaaggg tcagtgattg aagtgaacgg cagttttctg cgtccggaac
     4261 cagtgtggta tgtcagcgag gctgccagcc acgaggatca tcttgcacag gtggaaaacg
     4321 caattgctcg cttgccggcg ccgatgatcc tctacaccac cgaagtggaa caggccaaat
     4381 tctggtacca gtatctgcgc gagcgtggtt atcggcgctg tggattgttc catggtgcga
     4441 cgccaatgca ggaacgagaa ggtcttatcc acgcttggcg taacgatggg ctggatatca
     4501 tggtggcgac ttcggcattt ggtgttggga tggataaaaa caacgtccgt tccgtcttgc
     4561 atgtcgcagt tcctgaaaat ttagatcgtt tctaccaaga gagcggtcgt ggcggtcgtg
     4621 atggtaaagc atcggtcgcg cacattattt tccatcacaa acagctacag gtcgcacgga
     4681 acattaaccg taccaaactg atcggtgcca gcaaaggata tttgcgctgg gccaaaatgc
     4741 accagttgcg cgagcagcat cagccgggtc agttcatcgt tccgctacgc gcgaagcatg
     4801 ctgacattcg catggattcg cagagcaatg tggactggaa cctgcgtacc ttactgttaa
     4861 tgcagcgggc cgggtttatc gatatcgcct atccgcctcc ggatcttagc gctatcgccc
     4921 cggacgagcg tgatgaagca aagatccggg cgtggtttga ccattatttc aaccatattc
     4981 aggtttccgt acgccgtgat ggccatatgg atgaagcgcg gtggcatgag ggcattcagg
     5041 cccaccgttc gcatgaactg gggatgcgaa aacagggata tagcgcgttg gaggagtggc
     5101 tcaatgaccc ttcaatctcg ttgtgccaga agttagcgca attttacacc ttggatggct
     5161 ttgtgccgga aatagcctgc ggcggatgcc cagcctgtcg gagtaaaggg tatcctccct
     5221 ttactccgac gctcggtcgt atcgcgcacg tgacgggcga ggcggtgcgg aacgtcatgg
     5281 gaaatgagca acgcgtttat tatcctgcga cgctgacgaa ccggctactg cttcgtcaat
     5341 ggagtgactg gattgcccga ctcctagcca atcgccagat tcaggctatc cgcgccagcc
     5401 agagcgtact cgcccaactt agcgcggtac tgcccgcagg tttgcctttc tggtgttcgc
     5461 ttgccgttga tgaagaaaac acctgctggg acgaactggt gctggtgttg cccgacgaag
     5521 cgatgccgga actggacatt ttcgccacca tcaatcgcat tattgttgcg ccggaacgct
     5581 tacaggaacc gggctaccgc gggcgtcgct ggtgggaggt tgataccagt gccgtggcat
     5641 tagaacaatt tcagaggaat attatctagt ggcaattacc aataacgctg ccgcagggag
     5701 ccagatccgt ctgctgtgca tgatcgaccg ggtactgaat cggcggaccg gacaggcgat
     5761 cccgatggta gatttgattg aattactgcg cccggagatg ctgcccgtca gcgatggcgc
     5821 gagaaaacgt ctgcccgccg aagtctcttt ctggacaaaa gaagggttgt ggaaaaatga
     5881 gagcgctggc ctgagtcaac agtcgccgct ttccagcgaa cgtaatctgc ccgcacgggt
     5941 tttgcgtacg ctaattaaca gtgagaaaac gacgccgctg ctgactggaa cgcgcggtca
     6001 gcccttgctg atgtgtgtca cctccatgct tgcacaggaa aaatatacgt tacgtggaag
     6061 cacgccgctg acaaaagagg cggtgccgct ggccgttggc cccatgatta ataataaatt
     6121 agcaggtgag gggcgacgta acctcaacgt ctctaacgaa gcagaatcct tcctgaatta
     6181 cgcgtatttt ctcggtttta cagaacctta tctgaatggc tgggtgatgg accctacccg
     6241 cgccattgaa ggaattttgg ataacctggg gcttaccgcc gggatgccaa ttcaggagtt
     6301 cctcagccaa ctggcggagt ttctgccaat gctggatggg ggagcctacc gtcagcaggt
     6361 agaaccgatg atctctgctg acaactggca accgctggag aaacatatga ttagtgcttc
     6421 tttgagccag gcgttgcttc gtcttgagct gactatgcag ttggttttca gttcgcgttc
     6481 tgatgacctg gacgccatgg tcctgcaagg tccggatggc agcctgcgtc gtatcagtac
     6541 cgttagccct ggggaggcaa gaaaatgaga atgatcgaat actggccttc cttgaaaaac
     6601 atcaatgaat gtattcgcac cgaggcggaa gagttggcgg agtacacctt actggccgtg
     6661 catcaaccgg tgaatttgct gcgtcgtgat aaagacggca acaacctcgg atatgcgaaa
     6721 gaagaggatc tgctcaacca ctttctggaa acgccgcgcc caatcccggt agtgggtaaa
     6781 gctggggtgg ggaaatcgca catcatccgc tggttggatg cgaaactgcg attgcgccag
     6841 gagtacaaag acaagaagtg gcacattgtt cgtattccca aaagtgccag cctgcgtgaa
     6901 gttctcacct caatgctggc tggcctggaa ggcgagatct tcgacgaggc gcgggaagac
     6961 atcaataagg tgtcggataa acgcactcct cgcgagattg ctgaatggct actgatgctg
     7021 atggggcaag agctgcgcaa cctgcatgaa cgcaacaagc aggatatgga agcactgaaa
     7081 aaacagatgg cggggagtac tcctgagcag ttaaaagcca tgaagcctca ggcgacacgg
     7141 ctccagaaaa tccacatcca cgctgaagat aatgcattgc caacgctgat taacgatgcc
     7201 tacttcaagc agtttttgtt gaaagaagag cactgcctct atcgtcttgc cagccgtctg
     7261 accaacggcg caaccagcag cgatctcgac gaaggggaac agcaacttca ggccaaagac
     7321 cttgatttca gctttaacat caatgacctc agtctgccgg cgcgtcagta tattcagaaa
     7381 agccgcctta atacgcatga ggacgggcgc aaagatgcct ccgagatcct caacctggtg
     7441 ctgggtaaag cgacgcaggc gctgtttaac cagctcttca acttccgtgg ccgcagtttc
     7501 tccgacctgt ttactcagat tcgtcaggcg ctacatgaaa agcatatgac gctgatggtg
     7561 ctggtagagg atatgtcgct catcaccgcg atcgaagatg tgctgatcga cagcctggaa
     7621 agggaaggga tccgagacgg gaaagaggtg ctgtgccctg tgtgttcagc cattgccgtt
     7681 actgacggtt atcaggggta tgcccgccgc cgtcagggga tgctggatcg cgcgaagggt
     7741 gaatgggtga tcgaagaggt agggagcggg cgcgaagaga cgcgactgcg tattgtcgat
     7801 ttttgtggtc gatacatcaa cgcggcccgc tttgggagcg aagccttatt acagcgctgg
     7861 gagcaagccg ccgataaatc tcactggcca cctgactggg aagccagcgc aaacggtgac
     7921 gaaatggccg aagtgtttgg gcgaagcgag caaggttttt ctttgtatcc cttcaacgct
     7981 agcgctatcc atgcgttggc agaggcattc tgccgcgacg atcgcaacga attaaaattc
     8041 aacccacgtc agatcatcaa ccagatactg ttgcgtgtac tacagcactg tcgccgtgat
     8101 gccgatgagg gacgctttcc acctccgcgt cttggcgata ttgctgctcc ggcagggctt
     8161 cgaagctggc tgttccgcca gggctttgcc gataccgagc gtgccgaatc cgtggtcgcg
     8221 ctgtggggtt atcccgccga ttccgacgcc actttggcca gtacgctgcc gcctgacgtt
     8281 gcgcgcagtt tcgatctgga agatctggcg caggtgctgg aaaacacaaa aagtgccccg
     8341 ctagttgaac aatcggtgat gccaacgcgg gtgaccaaag ttgagcagca ggttaaaaaa
     8401 accgtgcagc cacctaaacc agtgaaaccc gtgctcaagg aggataaaga taccttggct
     8461 atccgcgcgc tggatgcagc ggtgggcgac tggatgctaa aaggcgcgcc gcttgagatc
     8521 gatcctgcac gatatatcaa aagctccctg gcctttttct ttgataagcg cgccgtggcg
     8581 gaatgggcgg gcagcagcta tcggccgacc ctttggttgg gtaaatccaa ctttgttgcc
     8641 gttgagttac ctaacgcgca gggtaaccgt ggcgtacatg tggtgaaatt tatttcccag
     8701 tccgaatacg acaagcgctc ggtgcaatta acggatgccg cgatggcgct ggcgcggttt
     8761 ggctactaca gagaaaactc cttaactaag accgaggact ggagctaccc ggagggcaaa
     8821 acggattacc tgattattca gagcttctgc gatcgctggg ttaactatgc gctgacggaa
     8881 ctcgttaagc ataagcgcaa cgatctgcca ctgcttctca gcgagcagat tgcacttgcc
     8941 gatgcgctgg gggtgattaa aacggccgat ggtccgaaag aggtgcttgg gcgactgctg
     9001 caaaacagca aaacgctttc ttcacagttc cgcaccggga tcacgaaggc aattaccgaa
     9061 ctgcgaggag aggcgttagc aaagtgggat gatgcccagg acgcatggct cagcctggtg
     9121 gcgctaaacg atcatgcgct ggaaggcgat ctcctgctga gtgcgataca aaaggtgtta
     9181 aaaaaacgct caaataacac acacgcagcc gtggtgaaaa agtcgctcag tgaaatccgc
     9241 cccgcactgg atactgctgc tctgtttgcc gactgtgaaa atgcggatga tttttccgaa
     9301 ctcgtcaccg gcctgactca gttggttaaa tcccttgggg atagcggtga ttatcctgcg
     9361 gatatgacac ccgacagtag tacgcttgcg aactctctga ctgggttaac ggaaggtgga
     9421 gtctggttga cgatcctcaa gttgcgaggc attacgcaat ctgaagatcc gcttcgccaa
     9481 tggcagttac tttgcgaact ggacggtact ttaattaacc gactcatatt cacaatcagt
     9541 ggctggcagc aggtcaacaa gcgggtgcta gcgaatatta ccgcctacaa ccattcgcat
     9601 ggcagtcacc agatcagcga atttcgcacg cagattgaaa gcacgttgca ggaacttcat
     9661 caggtcctcg acgccatgca gtccgtcgca ggggaacagt atgacaacgc ttaagaccca
     9721 acttacgcaa ctgcgggagc agtatcagat aaaacagcag gttcagtcgc tggaagaggt
     9781 ctacgatcgg taccgcacca ttcgtgacgg cttgctggat gttgccgaac cgctgcaaaa
     9841 agcccaaaag caacttgaag tgatcggaac gctaccggaa ccacactccg ctatcgattt
     9901 ttcgcaagag gcggcgcttt tcggcggtgc caaaacgcag ttggatgcgc tgacggcgcg
     9961 cttcaaagaa agtggcgata aaaccgagca gtgcgggttt tgggaaacgg tgcgggttct
    10021 gaagagcgta catgaaagta ttgatgataa ggtggatgca acctggaaag cgtttgttgc
    10081 cgatcttgaa gcgcgcgcaa cgctggatcc cgtctttctg gaacaacagc ggacgctggg
    10141 gaacgtgttg gtgtatgacg attatcgcaa agcgcgcgat aactttaatc ggcagaagtt
    10201 aagcgggcca gacagcctgt cggtggtaaa aaacctgcag aagtatagcg atgagatgat
    10261 cgcgcttaaa cgttcaatgg acctggatgc gccagacgat gttctgcgct tttttgaggc
    10321 gctggacaga cataatcatg cctcgcttac gctgctgacg ccgggcgtga ttacctggct
    10381 ggaagaaaaa gatatgctgc cagcgtttaa cgtgacacgt aagagaatga tttaatgaag
    10441 ggaatcgatc tttcgctttt gcaacgcgtg ctagatgcac tggaagaaca agaaaatgcg
    10501 ctcctggtat ggggtgacac gggcggcttt tttaatctgg atgaactttt aagcgttatc
    10561 ggacgggttt tacctgacga agatgccgaa gtggttctgg atcaaatgct agaggccgct
    10621 atgctgatcc aggtgccgca cgtcagtggc acgcctgtct ggcgaacccg aatgggggag
    10681 acggtccatc tcatgcgcaa cctgcgccag tggatgcata agcaaaaact ggaccagagc
    10741 aaaacgctgg tatcggacta tcgctttatt cgccggccgc gcagctatcc cgaccgcgtt
    10801 tacgaaccgg caacgctgat tagcggctgg aaaaaacagc tgaaattaag cgatcgcgtt
    10861 tgtgacatca tgcgccaggc gctggcgtca gataagccct tccttctggc gggttttcag
    10921 gtgcgcgcta ccgaacgcat tatgcagtgc tggcaggatc atcaggtgaa aagcaacacg
    10981 gccagcggga ccattgtctg tgctggcacc ggtagcggta aaacgctgtc gttttacctg
    11041 cctgcgctaa cccgtctcgc tgaggatatt tgcaacaacc ctgagcgcaa ggtgcgcatt
    11101 ctcgccatct atccgcgaaa agagttgctc aaggaccagt ttgccgaaac tttttcacag
    11161 tgccgcaagc ttgacgacta tatgctcacg gcggccggaa gaaaaattcg tatcggtgcc
    11221 ttttttggcg atacaccggt gaaggcggaa tggtctaagg aagacgttaa ggggaaggtt
    11281 ggtctgccgt tcgggttgat gaaatgccag cagccccacc cgcatcatcc taagcagagg
    11341 tgcggagggg ctctcatttg gcgtcgtgag gacgtatttg gcgagcgtga ggtacttacc
    11401 tgtacgcagt gccagcatca ggtggatcag agtgaaatca tgatcacccg tgatgcgcag
    11461 caacatcgcg gcgacgcgcc ggacattctg tttaccacaa cggaaatgtt gaacctccag
    11521 atgaactcca cctggagcaa ccacctgttt ggcgtcggcg agggctatgg gccgacgctg
    11581 gttctgctcg atgaggtcca cacctacagc ggaaccaccg gcgcgcaaac tgcgtttctg
    11641 ctgcggcgct ggatgcagcg caccgattgt ttgccgcatt ttgttggcct gtctgcaacg
    11701 cttaccgacg cgcagcactt ctttgccagg ttggtgggtg ccgctgagga gcaggttgcg
    11761 ctgatccagc cctacatgga agatatgatc gaagaggggg ccgagtacct gctggcgctg
    11821 cgcggcgatc cggtttctga aacggcatta ctctccacca ccatccaggc cagtatgcta
    11881 atggcgcgca tgctggatag caaagcgaat aagtcgcaag gtacgtgggg caagaagacc
    11941 ttcatcttca ccgacactct cgacggtaac aaccgtttgt accatgacct cagcgatgcc
    12001 gaaggttggg agacgggccc agggcactcg cgcattgatc actcaccgtt ggctgtgtta
    12061 cgtagccctt ttgataatac tgcacctgag cggtctaaaa ccgaactggg gcaaaactgg
    12121 agggcggcca cggatattgg ccacgacctc gcggaaaaca aagtcatttc ccgaacgtcg
    12181 agccaggatg ccggcgttga tgcatcagcg gatgtcgtgg tcgccacctc ttcgctcgag
    12241 gtggggtata acgatccgct ggtcggtgcc gttcttcagc ataaggcgcc gaacgatgtc
    12301 gcctcctatt tgcagcgtaa aggtcgtgcc ggacgtccac gaggcatgcg tccctggatg
    12361 ctggtggtgc tctccgagtt tggccgcgat cgcgtcgagt tccagcgtta tgaagggcta
    12421 atgagcccgg agatcaaacg tcagggtctg ccgctggaca accagcacgt acagaaaatg
    12481 caggcagcaa tggcaaccct cgactggatc agcaaagtcg gtcagttcaa agatctctgg
    12541 agcatgttga aaaaagctga gcataatcag ctgaaataca atcgaatgta tggcccgctt
    12601 atcaaactga ttgaagaggt gctgagcggc ggcaggcggt tgaacgaatt gatgcgctac
    12661 cttcaggatg ctctgcaatt atctgatggc gcggtgcaga atatcctctg gagcccgccg
    12721 cgctcgatca tgtttgagtt tctaccaacc atactgcgta acctcagaac gcgctggtcg
    12781 gttaacggcg tggagtgggc cggactgcgt ccgaatcagc caaatagcga gggcgaacag
    12841 caccggagca attcacccgt cccggaatac atcccgcaga acctgtttag cgagctaaac
    12901 ttgccggaac tggacattcg cctgaagcgt ggatttgacg atgaagacca ctgggaaact
    12961 ctctctttct ggcagggaat acgtgaattt gcgcctgggc gactctctaa acgctatgcc
    13021 gtcaaatcga acaaaagtac cgactggctg gtgccacaat cctacgagcc gatggcaggg
    13081 gaagggcggc aatttgttga tttccagata agcgatgcgt tcggcgatag ctggcaaaac
    13141 gagtatgaag ttgactatat gggaaaaacc atcaaggttg ttaaaccgtc gaaggtcatg
    13201 accactcggg ccgacattcg ccggatcaat gataaaagta atgcgcaact gcaatgggtg
    13261 ttcaacgtca ttaaccccgc tattgctacg ccagacgagg tgccgaaggg accctggaag
    13321 cacacgctga gcgatgtcac gttctacaac caccagcata tgaccccgct ggaactggtc
    13381 cgcttttcta ctggatcgca ggcgtcgctc cggttcagga ataaggaacg ggctcacgtt
    13441 gatttcacct gggttaatgg cgaggaacag gttggcgtag gaagtcgtca atgggtggat
    13501 gcaatgcgcc tgcgttttaa cctcacctgt gatgatgtgc ttggtctgct acatcaagaa
    13561 gaaatccagc gaggcatgcg ccccgtttat tttcaacatc ttgtaagaca gtcgccggaa
    13621 tttgaatttg acagcttcaa cgcggactgg gccattgagt gttttatggc tcagcttgcg
    13681 gagacactgg caaacggcgc tcacgcctcg gttgagtcgg cattacgaga aatggcctcc
    13741 gaaaaagggg gagaaaggct ggcagatatt ccggccagcc tgttccagcc ggataccgac
    13801 aatgaaacag gcactgatca ggcgctacag attggcctca acaaacttct tcagcgaccg
    13861 gaaatacagc agttgctgct gaactgcgcg caagcgctct ggaaacccct ggatgaaatc
    13921 gacggttttg ttgaatgggc acgccaggta ttagcggaca ccctggctgc gggtgtgcag
    13981 caaacgctaa gcacgctgct tccggacgtt gatgagctag cggtagtgac ggattccagt
    14041 tggatgagcg atccccgcaa gggagcagaa tggctggaaa tctggctctg tgagatggag
    14101 tctggcggca gcggtattct gattcgttta cagcagaagt gggctgaaga tcctgtctcc
    14161 ttcctcaatg tactagtgcg taatctttca gccagcgact acgagcagat cgattacgat
    14221 ctgcgaactg tgttgcagat gatgcaaacg gatgacacgc ttcgtatggc gatctcagcc
    14281 gtacgtgaag ccagcaacat ggacgcccgg cgggaggcta acaagaatct gcatttgcag
    14341 ctttcacagc gcggtatgcg cctttcccac agctttacca cggtgctgta ctcgcggatt
    14401 ctgcgggcgg gcagcggcga gcacacagat acccagcttt atcagctact gtcggactgg
    14461 ttgtcgcttg aagcgagcgc gggtattgaa ttcagtatga acaccatggc gcacgcgttg
    14521 gcggtgaact cgcttggcgt agaaactgat gcttccgtcg tgtttgacga gcagtgccgg
    14581 aatcaaaacc tgctctggcc acgaggctat acgatccgac aggctgagct cggcttttac
    14641 aatatgtttt gctcgcgcaa agtcacaacc gagcgtctgc ttgctggcgc gttatttagc
    14701 gagcaaatcg aaaagatcgc ccttgacgat gactggcttg ggcatttgca tgcggcgctg
    14761 cgtaaaggcg gtcgtgcgga actgatcctc actcgccagc agcgaaatca attacatcag
    14821 gtcattacca ccgtgcaaat tgaacccgtc gatcatttgg gactgctgct gtatccgcgt
    14881 ctcggtgaag tccgccgtga gcaggatgtg ctcatattgc gtattgaact ggcagaggcg
    14941 atgcaatgat cggacgtgca aaacagcggc agattttttt acatggctcc gcagggcacc
    15001 ggcagttgcg agaagtcctc agcgcccagg ttgttgctct gattatgcag ccagagccaa
    15061 tctggctggt ttcgccgtgg gtgagtaact tctcactact ggacaatcgc tcaggcaatt
    15121 gggacagcat tgatcccgcg tggggaggcc gggaggtgga tttcattgag ctgttagcct
    15181 gtgcggtgaa taacggcgcg ccgctttcgc tggtcacccg cgacgaaccg atgaaccgta
    15241 tctttgtaaa agcgctggaa aaacgcctga gtaactttgc gacctttcgt ttggaatggc
    15301 gtaaccagtt gcatacaaaa ggttttctga cgccaaattt ttttctcaag ggctcgatga
    15361 attttacccg gagcggtgtt aatcgaaatg aagaatttgt cgaactgaac tgcgattcgc
    15421 atatgatcgg tgaagcgatg gccgaatttg aaagtcagta tctttttatt ggatcaaagg
    15481 aagcgttctg atgtgtgcag actggctgaa aaattattac gctgaagata actatctgga
    15541 ttatgacaag gcgatgcagg gggggtatgg aatcccacaa atgaatacgc tgattcaaca
    15601 ggccgcagcg ctacggatgc cctgtatcgt tccttcaacg cgaacgggaa gaacggtgtt
    15661 ttatgctctg gctgagaatg cgaaaagcct agacgaatta cgacgtattt taaccgcggc
    15721 gttgggttcc gctgacacta ctccggatat taaatcactc aatcattctg acgatggcgg
    15781 ggagcagttg ctgctggaga aatccccgga cggaattctg gccttcgatt tcctaccggt
    15841 gccggacggt tcgccgcaac aggtaaaaga atggcaggca gcacgaatga aacgggttta
    15901 tgccatgctg caaacggtga tggatcttta tcatcagcga cctgtcttac attcgctggt
    15961 cagtcggcaa accggacgaa ttctcaggga tttctacaca gcatgccagg ctcgcgatgg
    16021 caagattgcc gaacagtatc tggaagagtt acgcggaaat cagacgcttt cttctctgaa
    16081 tctcctgttt cttgagctac aggggatggc ggcttcagca aaatgggacg cgattctcaa
    16141 ccaccctcgg ctggaagtat tattgcgcgg ccgggtgccg gagcgtatcc agcgcctgct
    16201 gttgcgatcc tctggccatt taatgcttaa cgccatccgc gatgcgcact tcccactgga
    16261 caggcgtgaa gatgccaggc gactggtgct gggcttattg ccgctgtaca agcataagcc
    16321 ccgctttgct catcaggcga gtttcctgcc tgactggcaa ctgtgggcca tgggggccgc
    16381 gctgctgggg atcgacgaat ggcaaacggc gacgcctcta ttagctcctg actggataca
    16441 gcaggtcgag gggtgggcga caggcacaac ctcgctgccc gcgccggtag cagcggaaga
    16501 acaggcgctg atccaggcgc cggttatcat gctgataagc cttgaaaacg cgaccgattt
    16561 attactcgaa gcccttctgg cggacgctga gcgcgaaagt gagatctacg cgcaactggc
    16621 ggcgatgcct gaagcaacac gacaggcatt gcaaaaaata cccaaactct gggaggcatg
    16681 gcaggcgctg aaaaaacgct gtgagcctca ggactatggc tggtgccgct ggcttgcaga
    16741 tttacaacag actacggaat ccgaacggtt tgaatcattg cgccagcagg cgaccgttca
    16801 ctacatggac tgggcaccgt caaccttcag cgaaacgcag tggcaggcgc tgcttgagca
    16861 tcagtccaac acccagttga gtaaggtttt gcgagacgta ctgccaactc ttctgaactg
    16921 gttggaagaa tatgacgtta aggtatctgc ctccttgtgg ccagactggc ttatgctgct
    16981 ggcagttgag gacatacgct cagaagaaga tgtacgtctc ggcgggatga ttctggataa
    17041 attcctttcc agtcccttta cctgcgaaga atattcggca gcgattgaat cgacagagat
    17101 tctatgtgct gaaaacgtgt cggtgaggac gttgggttat tctcttgata ttgctgaact
    17161 tctgtatgac agggtcactg caaacgaagc ctccagatta ggtttctgga ttaagttgca
    17221 ggagttactc aagcatcgct gggagcgcct ggatgccagt atgcagtttt ctgcacgcat
    17281 ggtggaaagg ctctatctcg gcgaacatgc cgggaacgtt ttcccgtcgg aggacaatac
    17341 gccgggcgta gcatcttcgc ttcatcgcga tctcagcggt aaaaccttag cgatttacag
    17401 cctgatgaaa ggggcggcaa gacgcggaaa agaggcgttg cttcagcttt atccaggact
    17461 ggaagtagag ctcaaccacg atcatgtcgc tacccctgcg ctggtgaatc tggcgaaaac
    17521 agcggactac tttatcttcg ctagtagcag ttcaaagcat caggcgttct atgctgtgac
    17581 ggattatcgc aaagagatca tctacccctc tggaaagggg gcgtccagta tgatcgcatc
    17641 tttcgtcagg gccatggagt aaataaggaa tagcctctgc tattccttat ttgttgtcac
    17701 ctctgaacca cctcctatgg aggtggtgat tgttcctgta gggctataac ccgaagaaag
    17761 cccatgcgga aatgtttcac cttatacccg gatgcttcag agaagcagtg cggtttttct
    17821 cggtaatgca accagaaaca atcctgaaga acgggtaata aatgttttaa gtctcctttt
    17881 aggggaccgc atcaaagcca ccttctaaga aggtggattc tttactattc accacggtaa
    17941 atgcagcctg cggagcatgt ctctttcact atcaccgcgc tcaggataga tagctgaggc
    18001 ttaatctgat tccaaatcca ttcagcaagc acttcgctgg tagggttctc cagtcccgtt
    18061 atatcattga gatagtagtg atccagtcta tccagagtgg gtttaaaaat cgcttttagt
    18121 tcggcaaagt cgataatcca accggtatgc ttatccactt cacctgttat ctcaaggcgc
    18181 acgacaaaag agtgtccgtg taagcgtccg catttatggc cattcggcac attgggaaga
    18241 cggtgcgcgg cttcaaaggt gaaatctttg tataacgttg ttttcatagg gacctcattt
    18301 tttgttatac cgtaacaata aggggcgata gttcctagca tttatgagtt aacttttaac
    18361 attctattgg gtaggcatga atatttaggt cgagtttcaa ctagactgtt ttttactgtg
    18421 ggacgtcatg taggatgagc atacacctaa attgtccgtt atcgctggta tcagttgcac
    18481 aacgaccacc tcactgacat cgacgaagat tggtgacaag aaagcatttc agcaacctca
    18541 tgatcccatc tttgttagct ggctgaagtc tccggtacgg caggcagagc aatagcattc
    18601 accatagtaa tttttcccgc attgtataca gttccagtcg gctttatata cccttggtgt
    18661 tgcatcggga aggaagcgtt gcaggcgtct tgcgaggtgt atttcactcg cgtctggctt
    18721 tacgtagggg caattgtgct gaccattttc ggcggaaccc gcatcaatgt gttcaaacca
    18781 cgggcgttca gtgtcgtatt caggatggta tttcagtgca ctgtgacaga ggtggcaggt
    18841 atagcggtcg taaggggcgg tctgtgccgt tctggcaccg gtaagacgat cgttaccgtc
    18901 gagagcgata aaagatttag caaacataat attactcccg ccagacaaca cgcaggcgta
    18961 agccgtccct gaaaggcgga cagacgccat gtggttgaat ggtttaaaag gattgagaag
    19021 ggaattgctg tgtggttgtc acactatggg atgttggcgt aaataaatcg ccaccagcaa
    19081 aacggatgta gagaatatca gccctggctg cagccgaatg agcgttcggt actgctgtat
    19141 cgcttggtgc gatgctttgc catatagctc aaacggaaag tcagtgcatc cagtttatcc
    19201 atgaaacccg gcttactgga tcaaaattta ccattgcgaa agcttgttct ggaaaagctg
    19261 gagatgaaat ggtctccaga gcaaatatca ggatggttaa ggcgaacaaa accacgtcac
    19321 aaaacgctgc gaatatcacc tgagacaatt tataaaacgc tgtactttcg tagccgtgaa
    19381 gcgctacacc acctgaatat acagcatctg cgacggtcgc atagccttcg ccatggcagg
    19441 cgtcataccc gcaaaggcga aagaggtacg attaacatag tgaacggaac accaattcac
    19501 gaacgttccc gaaatatcga taacagacgc tctctggggc attgggaggg cgatttagtc
    19561 tcaggtacaa aaaactctca tatagccaca cttgtagacc gaaaatcacc ttatacgatc
    19621 atctt
//
LOCUS       DAZFZC010000088        19305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFZC010000088 DAZFZC010000000
VERSION     DAZFZC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.089"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(224..1873)
                     /gene="pgi"
                     /locus_tag="ACHKXP_003730"
     CDS             complement(224..1873)
                     /gene="pgi"
                     /locus_tag="ACHKXP_003730"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO7648656.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2110..2306
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2398..3747
                     /gene="lysC"
                     /locus_tag="ACHKXP_003731"
     CDS             2398..3747
                     /gene="lysC"
                     /locus_tag="ACHKXP_003731"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO7648657.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4000..4272
                     /locus_tag="ACHKXP_003732"
     CDS             4000..4272
                     /locus_tag="ACHKXP_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO7648658.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4405..5277)
                     /gene="rluF"
                     /locus_tag="ACHKXP_003733"
     CDS             complement(4405..5277)
                     /gene="rluF"
                     /locus_tag="ACHKXP_003733"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO7648659.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5625..6572
                     /locus_tag="ACHKXP_003734"
     CDS             5625..6572
                     /locus_tag="ACHKXP_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO7648660.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            6642..7448
                     /locus_tag="ACHKXP_003735"
     CDS             6642..7448
                     /locus_tag="ACHKXP_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7648661.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDILGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            7458..7865
                     /locus_tag="ACHKXP_003736"
     CDS             7458..7865
                     /locus_tag="ACHKXP_003736"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO7648662.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            7865..8359
                     /locus_tag="ACHKXP_003737"
     CDS             7865..8359
                     /locus_tag="ACHKXP_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO7648663.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8425..9222
                     /locus_tag="ACHKXP_003738"
     CDS             8425..9222
                     /locus_tag="ACHKXP_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO7648664.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQAPASTALDQLDD"
     gene            9233..10057
                     /locus_tag="ACHKXP_003739"
     CDS             9233..10057
                     /locus_tag="ACHKXP_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO7648665.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10109..11338
                     /gene="sorE"
                     /locus_tag="ACHKXP_003740"
     CDS             10109..11338
                     /gene="sorE"
                     /locus_tag="ACHKXP_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO7648666.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11721..12410
                     /gene="pepE"
                     /locus_tag="ACHKXP_003741"
     CDS             11721..12410
                     /gene="pepE"
                     /locus_tag="ACHKXP_003741"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO7648667.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12500..14131)
                     /locus_tag="ACHKXP_003742"
     CDS             complement(12500..14131)
                     /locus_tag="ACHKXP_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO7648668.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14351..18034)
                     /gene="metH"
                     /locus_tag="ACHKXP_003743"
     CDS             complement(14351..18034)
                     /gene="metH"
                     /locus_tag="ACHKXP_003743"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO7648669.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDPDTMAKHIREWAEAGFLNIVGGCCGTTPHHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18234..19058
                     /gene="iclR"
                     /locus_tag="ACHKXP_003744"
     CDS             18234..19058
                     /gene="iclR"
                     /locus_tag="ACHKXP_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO7648670.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4547 a         5031 c         5055 g         4672 t
ORIGIN      
        1 ccgttaaatc acagacaagg ggatttatct gataaaaaaa tgccgacata acgtcggcat
       61 tgttattaag gttatttaat caatcgtgct atgtattaat tgccgaatac gatgtacata
      121 tcggcatcga cctgtaggcc tgataagacg cgaccgcgtc gcatcaggca tcggttgccg
      181 gatgcggcgt gaacgcctta tccggcctac atatcgacga tgattaaccg cgccacgctt
      241 tatagcggtt aatcagacca ttggtcgagc tatcgtggct gctgatttct ttatcatctt
      301 tcagctctgg cagaatacgg ttcgccagct gtttacccag ttccacgccc cactggtcga
      361 aggtgaagat gttcaggatc acgccctgag taaagatttt gtgctcatac agcgcaatca
      421 acgcacccag gctgaacgga gtgatttcac gcagcaggat ggagttggtc gggcggttac
      481 cttcgaatac tttgaacggc accacgtagt caagcgttgc cggatcttta ccctgatcac
      541 gatattcctg ctcaaccact tcgcgggatt taccaaacgc cagcgcttcg gtctgggcga
      601 agaagttaga cagcagtttc tggtgatgat cagagagcgg gttatgggtg atagccggag
      661 cgatgaaatc gcacggtacc attttggttc cctggtggat cagctgatag aacgcgtgct
      721 gaccgttagt acctggttca ccccagataa tcgggccagt ctggtaatcc accacgttac
      781 cgttacggtc aacatactta ccgttggact ccatattgcc ctgctggaag tacgccgcga
      841 aacggtgcat atactggtca tacggcagaa tcgcttcagt ttccgcacca aagaaattgt
      901 tgtaccagat gccaatcagc gccagcagga caggcaggtt tttctcggca ggcgtggtgg
      961 agaaatgctt gtccatcgcg tgtgcgccag aaagcagttc aacgaagtta tcaaagccga
     1021 tggagagaac aatcgacagg ccaatcgctg accacaaaga gtaacggccg ccgacccagt
     1081 cccagaactc gaacatgttg gcagtatcaa taccaaactc gccaacggct ttggcattgg
     1141 ttgagagcgc cgcaaagtgt ttcgcaacgt gtttttcatc acctgccgct ttcaggaacc
     1201 agtcacgcgc gctatgggcg ttggtcatgg tttcctgagt ggtgaaggtt ttagatgcta
     1261 ccaggaacag cgtggtttcc gggtttactt ttttcagcac ttccgcgatg tgagtcccat
     1321 cgacgttaga aacaaagtgc atgttcaggt gatttttgta cggacgcagt gcttcggtca
     1381 ccatgtacgg gccgaggtca gaaccgccga tcccgatgtt cactacgtca gtgattgctt
     1441 tgccggtata acccttccac tcaccggaga taatcgcttc cgagaaggtt ttcatcttct
     1501 ccagcaccgc gttgacttct ggcataacat ctttgccatc aaccagaatc ggggtattgc
     1561 tacggttacg cagcgctacg tgcagcacgg cgcggttttc agtgcggttg atcttctcgc
     1621 cagagaacat cgacttaatc gcgcccgcca gatcgcactc tttcgccaga tcctgtaatt
     1681 tcgccagcgt ctcttcagta atgcggtttt tggagtaatc caccagcatc tgatcgtcga
     1741 atgttgcgga gaacttagaa aaacgatcgc cgtctttagc aaaaagatcg gcgatcgtaa
     1801 cgtctttcat ttcatcgaag tgtttctgta gtgcctgcca ggcagcggtc tgcgttggat
     1861 tgatgttttt cattagcaat actcttctga ttttgagaat tgtgactttg gaagattgta
     1921 gcgccagtca cagaaaaatg tgatggtttt agtgccgtta gcgtaatgtt gagtgtaaac
     1981 ccttagcgca gtgaagcatt tattagctga actactgacc gccaggagtg gatgaaaaat
     2041 ccgcatgacc ccgtcgttga caaccgcccc gctcaccctt tatttataaa tgtactacct
     2101 gcgctagcgc aggccagaag aggcgcgttg cccaagtaac ggtgttggag gagccagtcc
     2161 tgtgataaca cctgaggggg tgcatcgccg aggtgattga acggctggcc acgttcatca
     2221 tcggctacag gggctgaatc ccctgggttg tcaccagaag cgttcgcagt cgggcgtttc
     2281 gcaagtggtg gagcacttct gggtgaaaat agtagcgaag tatcgctctg cgcccacccg
     2341 tcttccgctc ttcccttgtg ccaaggctga aaatggatcc cctgacacga ggtagttatg
     2401 tctgaaattg ttgtctccaa atttggcggt accagcgtag ctgattttga cgccatgaac
     2461 cgcagcgctg atattgtgct ttctgatgcc aacgtgcgtt tagtagtcct ctcggcttct
     2521 gctggtatca ctaatctgct ggtcgcttta gcggaagggc tggaacctgg cgagcgattc
     2581 gaaaaactcg acgctattcg caacatccag tttgccattc tggaacgtct gcgttacccg
     2641 aacgttatcc gtgaagagat tgaacgtctg ctggagaaca ttactgttct ggcagaagcg
     2701 gcggcgctgg caacgtctcc ggcgctgaca gatgagctgg tcagccacgg cgagctgatg
     2761 tcgaccctgc tgtttgttga gatcctgcgc gaacgcgatg ttcaggcaca gtggtttgat
     2821 gtacgtaaag tgatgcgtac caacgaccga tttggtcgtg cagagccaga tgtagccgcg
     2881 ctggcggaac tggccgcgct gcagctgctt ccacgtctca atgaaggctt agtgatcacc
     2941 cagggattta tcggtagcga aaataaaggt cgtacaacga cgcttggccg tggaggcagc
     3001 gattatacgg cagccttgct ggcggaggct ttacacgcat ctcgtgttga tatctggacc
     3061 gacgtcccgg gcatctacac caccgatcca cgcgtggttt ccgcagcaaa acgcattgat
     3121 gaaatcgcgt ttgccgaagc ggcagagatg gcaacttttg gtgcaaaagt actgcatccg
     3181 gcaacgttgc tacccgcagt acgcagcgat atcccggtct ttgtcggctc cagcaaagac
     3241 ccacgcgcag gtggtacgct ggtgtgcaat aaaactgaaa atccgccgct gttccgcgcg
     3301 ctggcgcttc gtcgcaatca gactctgctc actttgcaca gcctgaatat gctgcattct
     3361 cgcggtttcc tcgcggaagt tttcggcatc ctcgcgcggc ataatatttc ggtagactta
     3421 atcaccacat cagaagtgag cgtggcatta acccttgata ccaccggttc aacctccact
     3481 ggcgatacgt tgctgacgca atctctgctg atggagcttt ccgcactgtg ccgggtggag
     3541 gtggaagaag gtctggcgct ggtcgcgttg attggcaatg acctgtcaaa agcctgcggc
     3601 gttggcaaag aggtattcgg cgtactggaa ccgttcaaca ttcgcatgat ttgttacggc
     3661 gcatccagcc ataacctgtg cttcctggtg cccggcgaag atgccgagca ggtggtgcag
     3721 aagctgcatt ttaatttgtt tgagtaaata ctgtatggcc tggaagctat atttcgggcc
     3781 gtattgattt tcttgtcact atgcttatca ataaacgagc ctgtactctg ttaaccagcg
     3841 tctttatcgg agaataattg cctttaattt ttttatctgt atctctaatt aattatcgaa
     3901 agagataaat agttaagaga aggcaaaatg aatattatca gttctgctcg caaacgaatt
     3961 cccgtacact gaactctctg gctcactgag ggcaccatca tggcactccc ccgtattacc
     4021 caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa cactgctgga tcgggcgcgg
     4081 attgcgcatg gtcgcgtact gaccaactcc gaaactaaca gcattaagaa agagtacatc
     4141 gataaattaa tggtcgagcg tgaggctgaa gcgaaaaaag cccgccaatt gaagaaaaag
     4201 caggcttata aaccagatcc tgaagcgtca tttagctggt cagcaaatac gtcgacgcgc
     4261 ggcaggcgct aatgaacaat caacgcctga gattttcact cgtctgtgtg gacacaatct
     4321 tcccctcacc ctaaccctct ccccgaaggg gcgaggggac tgtccgagca ctttttggac
     4381 gttttcatca gtctgaggcg ttaatcagcg ccccttcttt tttcgccccg gcgaggtaaa
     4441 acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc gttttttcca tcttaacgac
     4501 tggacgtttg atgcccgctg ttttcggttt ggccttcgct ttaggttttg cctcggaaga
     4561 ggaattttcg atgagcttaa agaggtcgat taactcatcg tcggttaaat cgcgccactc
     4621 ccccagcgga atgccgctta agctgacgtt cataatgcgc gtacgttcca gctttttcac
     4681 ttcatagccg aaatgctcgc acatgcgccg gatctgacgg ttcagcccct gcaccagggt
     4741 aatgcggaag acaaatggcg cttctttttt gactttgcac tttttagtca cagtcccgag
     4801 gattggcacc cccgccccca taccacgaat aaattcatcg gtaatcggtt tatcgaccgt
     4861 caccagatac tctttctcat ggtcattacc cgcgcggagg attttattca ccagatcgcc
     4921 gtggttggtg aggaaaatca gcccctggga atctttatcc agacgaccaa tcgggaacac
     4981 gcgtttgctg tggttaacga aatcgacaat gttatcgcgc tcgccatctt cggtggtgct
     5041 gacaataccg acgggcttgt tcagggcgat aagtaccaaa tcttcggctt cccgaggctc
     5101 aatcaactga ccgtttactt tcacaacgtc gccgggtttc acctgatcgc caatggtggc
     5161 tcgcttgcca ttaaggaaca cattgccttg ctcgatatag cgatccgctt cgcggcgtga
     5221 gcaaattccg ctttcgctga tgtatttatt taaacggact gatgagtcgg gcagcataaa
     5281 ttctcctgta aaacgcggaa tataccgtac ctcttagcgg ggaaaaacag ggttatgcgg
     5341 caacgtcgaa attaaatgtg atctgccacg ccttttatca caaaagtgtc tccggaagta
     5401 caatgcaaat ttgcctggca aaataaggat aatcaacata ttacgaatcg ctgcacacct
     5461 gtgctgatga ctactgcgca gagaaacaaa tgtgcaagag aaagcgctat ttctccccat
     5521 gcaaagagtg aaaaatcgct ttgaaaggcc ctcatgatgg ggttatatca gatcaagcgg
     5581 tgagaaagcg tatttgcaca aatgtgcaga accaggaggc ggcaatggaa aacagtgacg
     5641 atatccgttt gattgtgaag attgcccaac tctattacga acaggatatg acgcaggcgc
     5701 aaatcgcgcg cgaactgggt atttaccgca ccaccatcag ccgcttgctt aaacgaggcc
     5761 gcgatcaggg aattgtcacc atcgccatca actatgacta caacgaaaat ctctggctcg
     5821 agcagcaact gaagcaaaag tttggcctga aagacgttgt ggtggtgtcg ggaaatgatg
     5881 aggatgaaga gactcaactg gcgatgatgg ggttacatgg cgcacaactg ctggatcgct
     5941 tgctggagcc tggcgatatt gtcggttttt cctggggtcg cgcggtgagc gcactggttg
     6001 aaaacttgcc gcaagcgggg caatcgcggc agttaatctg cgtgccaatt attggcggac
     6061 cgtccggtaa actcgaaagc cgctatcacg taaacacatt aacctacagc gcggcagcga
     6121 agctgaaagg ggaatcgcat ctcgcggatt ttccggctct gctggataac cccttaattc
     6181 gtaatgggat catgcagtct cagcacttta aaaccatctc tgcctactgg gataatctgg
     6241 atgtcgccct ggtgggaatt ggctcaccgg ccattcgcga cggcgctaac tggcatgcgt
     6301 tttatggtgg tgaagagagt gacgacctga atgcccgcca ggttgctggc gatatttgct
     6361 cgcgcttttt tgatattcac ggcgcaatgg ttgaaacgaa tatgagcgaa aaaacactct
     6421 ctatcgaaat gaataaatta aagcaggcac gatattccat tggcattgcc atgagtgaag
     6481 aaaaatacag cggaattatt ggtgcactgc gtggaaaata tattaattgt ctggtaacga
     6541 atagcagcac agctgaacta ttactgaaat aaaaatcttc ccgatgttta ccgtaataaa
     6601 aattacggcg ggtccctatt atctaaaatc aggagtctgt tatgcaaacg tggttaaatt
     6661 tgcaggataa aatcattatc gtcaccggcg gcgcatccgg tattggtttg gcgattgtag
     6721 aggaattatt agcacaaggc gcaaacgtac agatggtaga tattctcggt ggcgatggtc
     6781 aatatgaagg tcataaaggc tatcagtttt ggccgaccga tatttccagc gccaaagagg
     6841 taaatcatac ggtagcggaa attatccagc gttttggtcg catcgacggt ctggttaaca
     6901 acgccggggt caatttcccg cgtctgctgg tcgatgagaa agcgcctgcc gggcagtatg
     6961 aactcaacga agctgcattc gaaaaaatgg tcaatatcaa ccagaaaggc gtttttctga
     7021 tgtcgcaggc ggtggcgcga cagatggtca aacaacatga tggcgtgatt gtgaatgttt
     7081 cctcagaaag tggactggaa ggctcagaag gccaaagctg ttacgccgct accaaagccg
     7141 cgctcaatag cttcacgcgc tcctggtcga aagaattggg taaacacggt atccgtgtgg
     7201 tcggtatcgc gccggggatt ctggaaaaaa caggactgcg cacgccggaa tatgaagaag
     7261 cgctggcgtg gacgcgcaat atcaccgtcg agcagctgcg tgaaggctat accaaaaacg
     7321 ccattcctat tgggcgcgcc ggaagattag cagaagtcgc tgattttgtt tgttatctgc
     7381 tgtctcaacg cgccagctac atcaccggag taaccactaa cattgcgggc ggcaaaacgc
     7441 gcggctaagg aggctttatg gtcaacgcca ttttttgtgc ccacggcaaa ttagcttgcg
     7501 ccatgctgga atcggttcag atggtttacg gcaacgccaa tgttgaagcc gttgaatttg
     7561 tgcccggtga aaacgccgga gacatcgtag caaagctgga aaagttagta agcattcaca
     7621 atcaagatga gtggctgatt gccgtggatt tacagtgcgg cagcccgtgg aatgcggcag
     7681 cgatgcttgc gatgcgcaat cctcgtctgc gggtaatcag cggcctttct ctgccgctgg
     7741 cgctcgaact ggttgataac caggacagca tgaatgttga tgaactgtgt gaacacctga
     7801 cgcaaatcgc aaagcaaacc tgcgttgtct ggaaacaact ggcaacgaca gaggaggatt
     7861 tctgatgaac attacgctcg cccgcattga tgaccgcctg atccacggtc aggtcactac
     7921 cgtctggtcg aaagtggcta acgcccagcg cattattatc tgcaatgacg aagtttataa
     7981 cgatgaagtt cgccggacat tattgcgtca ggctgcgcca ccgggaatga aggttaacgt
     8041 ggtcaatatt gaaaaagccg tcgcggttta tcacaacccg caatatcagg acgaaaccgt
     8101 tttttatctt ttcactcgcc cacaggatgc cttagcgatg gttcgccagg gggtaaaaat
     8161 aggcaccctg aatattggcg gcatggcctg gcggcccggt aaaaaacagt taaccaaagc
     8221 ggtttcatta gatgatgatg atattaacgc atttcatgag ctaaataatc ttggcgtcat
     8281 actggattta cgcgtcgtag cgtcagatcc ctcaatcaat attatcgaca aaataaacga
     8341 acagttaatc gcaaattaaa taatagcgcc tgtgtacata tgctttaaca ggcagggatg
     8401 acgacactta aaaggtgaca aatcatggaa ataagtaccc tacaaattat tgctatattt
     8461 cttttttcct gtattgccgg aatgggcagc gtgctggatg aatttcagac tcatcgcccg
     8521 ttaattgcct gtacggtgat tggtttaatt ctcggtgatt taaaaaccgg aataatactc
     8581 ggtggtacgc tggaattgat agctctcggc tggatgaacg tcggcgcggc gcaatctccg
     8641 gattctgcac tcgccagcat aatctccgcc attctggtta tcgttggtca gcaaagcatc
     8701 gccaccggaa tcgccatcgc gttgcctgtg gctgcggcag gccaggtgct gacggtgttt
     8761 gcccgtacca tcaccgtagt gttccagcac gcggcggata aagcagcgga agaggcgcgg
     8821 tttcgcactc tcgatattct gcatgtctcc gcgcttggcg tgcaggcgct gcgcgtcgcc
     8881 attccggcac tgattgtctc gctgttcgtc agcgccgata tggtcagcaa tatgttaaac
     8941 gccattcccg aatttgtgac ccgaggtctg caaattgctg gcggttttat cgtggtggtc
     9001 ggttacgcca tggtgctacg tatgatgggc gtgaaatatt tgatgccttt ctttttcctc
     9061 ggtttcctcg caggtggcta cctcgatctc agtctgctgg cgttcggtgg cgtcggcgtg
     9121 atcatggccc tggtctacat ccagctaaat ccacagtggc gtaaagctga acctcagctc
     9181 caggcccccg cttccaccgc ccttgaccaa cttgacgatt aacggagcca tcatggaaca
     9241 gagaaaaatt acacgcagcg atctggtgag catgtttctg cgctccaacc tgcaacaggc
     9301 gtcctttaac tttgaacgta ttcacgggct gggcttttgc tacgacatga tccccgccat
     9361 caagcgactt tacccattaa aagaggatca ggttgcggcg ctcaggcgac acctggtgtt
     9421 cttcaatacc acgccagccg tatgtggccc ggtcatcggc gtcactgccg ccatggaaga
     9481 ggcgcgggcc aacggcgcgg aaattgatga cggtaccatt aacggcatca aagtcggtct
     9541 gatgggacca ttggcaggag ttggcgatcc actggtttgg ggaacgctgc gcccgattac
     9601 cgctgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc tgctgttctt
     9661 ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc tcggctttcg
     9721 caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac tcaccgaagg
     9781 cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtc accaaatgga cgtcaatcaa
     9841 cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca ccatgaccgt
     9901 gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga cgctactgat
     9961 ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc tgtttggctt
    10021 agggattatc ggcaatgcgc tgggcttcct gtcttgattc ttcgccccgg cacgactgcc
    10081 ggggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc tttatggtaa
    10141 acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg aaatcctcgc
    10201 gacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaacc tgggtgaaaa
    10261 ccataaaaaa gtacccgacg atgtggcgac caaccccatc atcatcggcc acgagttttg
    10321 cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg gtcagcgtta
    10381 tgtgattcag gccaacctgc aactccccga ccgcccggac tgccccggct actccttccc
    10441 gtgggtgggc ggcgaagcca cgcatgtggt tattcccaac gaggtcatgg aacaagattg
    10501 cctgctggca tacgacggcg aaacctattt tgaaggctcg ctggttgaac cgctttcctg
    10561 cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata accacacgat
    10621 ggggattcgc ccgcaagggc gcatgctgat cctcggcggc accggaccaa tgggactgtt
    10681 ggcgattgat tatgcgctac atggacccgt taacccgtcg ctgctcgtca ttaccgatac
    10741 cgacaacgat aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc aaacactgat
    10801 tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga gtggcggtca
    10861 cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc tcgcctcttc
    10921 cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata aacatttcag
    10981 cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg gtacgtcagg
    11041 cggcaatacc gacgacatgc gcgcagcggt caaactgatt gaagagaaaa aagtgcaggc
    11101 cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca cgcttgaatt
    11161 gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc cgctgacgtc
    11221 actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc aggggatctg
    11281 gtccggtgag gcggagcaat atctgctcac tcatgcagag gcaatttccc atgattaatc
    11341 gcgctactta actctgcatg tcgctggctg gtcgccgcgg caacttcgcg acctgatgtg
    11401 cggctgattg ctgaagcggc ccaatgtgca cgagaaggat aaaaaaacat cagcgcctta
    11461 aggggcgctc aaattggtgg caaagtacac attgtttatg ccggatgcgg aataaagcct
    11521 tattcggcct acataatctc gaaaattcaa tatattgcag ttatctggta ggcctggtaa
    11581 gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc agcgcccggt gcaatacgtg
    11641 acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc ttccgtgatt
    11701 tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg ctgccgggta
    11761 aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt cgccgctcag
    11821 cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca gagaaaacgg
    11881 ctgcggttct tgctccgctg ggtgtttctg tcaccggtat tcatagcgtt gtcgatcccg
    11941 ttgccgcgat tgaaaatgct gaaattgtga ttgtcggcgg cgggaatact ttccagttgc
    12001 tgaaacagtg ccgcgagcgc gggctgctgg ctccaattac tgacgtggtt aaacgtggcg
    12061 cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt cgtaccacca
    12121 acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct gaatctgttc ccgttgcaaa
    12181 tcaacccgca cttcaccaac gcgttgccgg aaggccataa aggtgaaacc cgtgaacagc
    12241 gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtcta ccggaaggta
    12301 actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc acttatgtgt
    12361 ttaaggctgg tgaagaagcg gttccgctgg aagcgggtca ccgtttttaa gtgaattgcc
    12421 ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg cgtcttatca
    12481 ggcctacagg gtcacggtat taatactcgt cccgtccttc atcttcatcc ggctgttcca
    12541 gcacactgta agccaccgaa caaaacagcg agttcagtcg ctgcatatct cccagtaagc
    12601 ctaaatgtag cgaactggtt tcaatactct gcacgttttg ctgatgcagg cgatcgacgt
    12661 gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcgg cgcaaacgac
    12721 gggcgctggt aacatcgcca gagaagaaaa ccgacattgc cagttttaaa ttactgagca
    12781 attgctcata gagtgcatcc agttccttca acccatcaag cgaaaatgcc cgccgtgctg
    12841 ccagtgattt gtcggcaatt tcactcccca tgcgctcgac gatatcggag gcctgttcaa
    12901 ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc gccagctctt
    12961 cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg atatcatctg
    13021 ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta ttcagccctt
    13081 ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg ttcgccagag
    13141 caagcgtggg cgtatccagc gcgctgacat ccagatgttt gggccgtagc tgggtatcca
    13201 gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca acaaatggca
    13261 gcatgaccag gcagcgtaca aggttgtaga agacgtggaa atagatcacc agttccgctt
    13321 ttggcagtgg caacttcccc attgtctctg ccagcaaatg gacaaacggc aggataatca
    13381 ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct gcggcattgg
    13441 cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagcacca atcaccagac
    13501 agagcgccac ggggaaggag ataatgcctg cggcggtcag agtcgcagtc agcagtacag
    13561 cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagtgca tccagcagaa
    13621 tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg atcggcgtta
    13681 cggcctgcac aatcaactcc agcgccagta aaatcagccc aagaccaata ccgacgcggc
    13741 ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg ataaaaataa
    13801 gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc gccgtcccga
    13861 catctgcacc cagcacaatg accagagccg gtgcgagggc taccagatcc tgagcgacaa
    13921 acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta acgccgatcc
    13981 ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta cgcagacgcg
    14041 cgccgaagac gcgcattacg cctgttcgaa caatatgagt cccccagacc agcagggcga
    14101 cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat cgttatatgt
    14161 tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc tttactgtgc
    14221 cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta taagtgtttc
    14281 atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg aaaagtgaca
    14341 gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc tcaacatctg
    14401 agacgctcat acctttacgg cgggcatagt cttcaacctg gtcgcgctga atttgcgcta
    14461 ccgcataata cttgctgtcc gggtggctga agtaccaacc agaaaccgat gcaccaggcc
    14521 acatggcaaa agattctgtg agtttcatgc cagtgtgttt ttccacttcc agcagctccc
    14581 agatggtggc tttttccgta tgttccgggc aggccgggta gcccggtgcc ggacggatgc
    14641 cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc gcatagcccc
    14701 agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct aaacggtcgg
    14761 caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag gcatcagcca
    14821 gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct gctttaccag
    14881 aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt ttttcggtct
    14941 gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca cggtagattt
    15001 caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc ggattcagcg
    15061 ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct
    15121 caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc gaccaggtca
    15181 taaagaacgg tgtccagtcg atgtaattac gcagagtttc gatgctggct tcaacttcct
    15241 gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaaa gcgaagtcgt
    15301 tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgcgg tttcttgcgc ccgtgctgaa
    15361 tacgtacggt ttcgtactcc ttgcgggtac gagcgacaaa atcatcgcgc tgagtatcgg
    15421 aaagcagcgc cgccaccaca ccaacggtgc gcgaggcatt ctgcacatac accgtcgggc
    15481 cgctgtagtt ctgctcaatt ttcaccgccg tgtgcgcttt tgaggtcgtc gcgccaccga
    15541 tcagtaacgg aatggtgaag ccctgacgct ccatctcttt cgccacgtta accatctcgt
    15601 ccagcgacgg cgtgataagc cccgaaagtc caatcagatc agcattcact tctttagcgg
    15661 tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg tagttgttac
    15721 attgcagcac cacaccaacg atatttttcc cgatgtcgtg gacgtcgccc ttcacggtgg
    15781 cgatcaccat cttgccgttg gttttgccct gctctttgct ggcttcaata aacggttcga
    15841 ggtaggccac cgcctgcttc atcacacgcg ccgatttgac cacctgtggc aggaacattt
    15901 tcccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg ccttcaatca
    15961 cttcaatcgg gcgcgtagcc tgctggcggg cttcttcggt atcctgctcg ataaactcag
    16021 taatgccttt gaccagcgag tattccagac gtttattcac ttcccacgag cgccactccg
    16081 cctgctgggc gttagcggtg tcgtcggttt tgctgccgcg atatttctcg gcaagctcca
    16141 gtaaacgctc agtgccatca tcgcgacgat taagaatcac atcttccacc gcgtcgcgca
    16201 gttcagcggg caggtcgtcg taaatagcca gttgcccggc gttgacgatc cccatatcca
    16261 tgccattgcg aatagcgtag tagaggaaca ccgcgtgaat ggcttcgcgc accggatcgt
    16321 tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg tgcggcagtt
    16381 cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg tgctcttcaa
    16441 tgccagttgc gaccgcgaag atgttcgggt cgaagatgat atcttctggc gggaagccaa
    16501 cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt gcgcgagtat
    16561 cggcctgtcc ctgttcgtca aaggccatta ccaccactgc cgcaccgtag cgacgcaaca
    16621 atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag ttaacaatgc
    16681 ctttgccctg gatacacttc agaccttttt caatgacgtc ccattttgag gagtcgatca
    16741 taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa cgcaccatcg
    16801 ccgcttcggc gtcgagcatc ccttcatcca tattgatatc gataatctgc gcgccgtttt
    16861 ccacctgctg acgcgccaca tccagtgcct cgctgtattt ctcttctttg atcaggcgct
    16921 tgaacttagc ggaaccggtg acgttggtgc gttcacccac gttcacaaac aggctatctt
    16981 cgccaatgtt cagcggctcc aggccggaca aacggcaggc tacgggaatt tccggcagtt
    17041 tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgatgt ggcgtggtgc
    17101 cacagcagcc gccgacgata ttgagaaagc ccgcttccgc ccattcgcgg atgtgctttg
    17161 ccatcgtgtc ggggtcgaga tcgtattcac caaaggcgtt gggcagaccg gcgttcgggt
    17221 gcgcggtgac gtagcattcc gcaatccgtg acagctcctg cacgtactgg cgcagttcat
    17281 cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc aatgagttgt
    17341 aaaatgcttc ggtggtttgc ccggagagtg tacgtccgga ggcgtcggtg atggtgccgg
    17401 agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc gcaaataccg
    17461 ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc gcaccacctt
    17521 ccaccagcgc tttggtggac tctcgataag ccgccaccag cccgtcaaaa gtgatattac
    17581 gaaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtc gggccgagaa
    17641 caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcatca gcacaggcgc
    17701 gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc tggtaatccg
    17761 ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg cccgcttcaa
    17821 agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc aggtcgttgt
    17881 tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg gcttcgttca
    17941 gtcgatagct ctggatcatg gtgcccatac cgccgtccag caccagaata cgttcattta
    18001 actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg ctcaacccag
    18061 atttaataaa aattcaacaa accatactgg cataaacgca tctgtggtaa aagcgaccac
    18121 cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa ctattgcatt
    18181 agctaacaat aaaaatgaaa atgatttcca cgatacagaa aaaggaggct gtcatggtcg
    18241 cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca gcgactggac
    18301 aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa tccaatggca
    18361 gtgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg acccaccgcc
    18421 tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt tggcgaactg ggacactggg
    18481 caatcggtgc acatgccttt atggtcggca gcagctttct ccagagccgt aatttgttag
    18541 caattgttca ccctatcctg cgcaatttaa tggaagagtc tggcgaaacg gtcaatatgg
    18601 cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag tgtacgcatc
    18661 tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc ggtgcgggta
    18721 aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt
    18781 tacatgccta tacccacgca acgctggtgt ctcctgtgca tttaaaagaa gatctcgccc
    18841 aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcactgggg ctacgttgcc
    18901 ttgcagcgtg tattttcgat gagcaccgtg aaccgtttgc cgcaatttct atttccggac
    18961 cgatttcacg tattaccgat gaccgcgtga ccgagtttgg cgcgatggtg attaaagcgg
    19021 cgaaggaagt gacgctggcg tacggtggaa tgcgctgact tttttctggc gggcagaggc
    19081 aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta atcgcattga
    19141 cgccaaaata acttaatgtc atacacttca cacactttta ttaactcggc attattttta
    19201 aacatcaaac cacttaatta ttaacaaaat cgcaagatct cattccaccg aaacttttac
    19261 ggctgcatta tgagattgag cgatttatga tatttcttta gttgt
//
LOCUS       DAZFZC010000089        19127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFZC010000089 DAZFZC010000000
VERSION     DAZFZC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.090"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(411..1247)
                     /locus_tag="ACHKXP_003745"
     CDS             complement(411..1247)
                     /locus_tag="ACHKXP_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7648671.1"
                     /translation="MTRLASRFGAANSIRRDRPLTLEELFRTVPSVFSEEKHESRSEK
                     YTYIPTITLLDSLQKEGFYPFFACQTRVRDDSRRDHTKHMLRLRRHDQITGSQVPEII
                     LLNSHDGSSSYQMLPGFFRYVCQNGLVCGDTFGEVRVPHKGDVVCKVIEGAYEVLGTF
                     DAIADKREEMQSLVLPPPAQKALAQAALTYRFGEEHQPITEEQVLQPRRWEDKKDDLW
                     TVYQRLQENLIKGGLSGRNAKGKRARTRSVNGIDGDIKLNKALWVLTAQMHAHFSGRE
                     RL"
     gene            complement(1307..1732)
                     /locus_tag="ACHKXP_003746"
     CDS             complement(1307..1732)
                     /locus_tag="ACHKXP_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7648672.1"
                     /translation="MNNTTESAIAMTLVPDEQRLDFWLNHFGSVKGWSTFEVVIFTTM
                     GQFCDDYQGGYWEYCTLSNGGAFIYPDLPQETLTLFNPHNGNEANVSCEAAGIAVCLM
                     MYSLWSFQTESDILVDHFYQLRDYAAQHPERSAIFHLID"
     gene            complement(1824..2696)
                     /locus_tag="ACHKXP_003747"
     CDS             complement(1824..2696)
                     /locus_tag="ACHKXP_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7648673.1"
                     /translation="MHHNAHFKTLLSTLPTSFQTSFFNQLSHLINYSPIIGLMGKTGA
                     GKSSLINALFQSSLSPVSDVSGCTRQAQRFSMTMNNHTLTFVDLPGVGESLERDKEYH
                     QLYRNLLPELDLIIWVLKADDRAWSSDEQCYRFLTKKCGYQPNQFLFVLNQADKIEPC
                     RQWNEHNHKPSSEQAYNLKLKQQAVITAFKPHHPVIPVSAVENYQLTELAEQLIQALP
                     AKASSGVARQLNIPYRTQSVENAARNDFGQCVSDIVDTLIDIIPLPPLIRSTVSTVKN
                     SIASVAKSLWRMFF"
     gene            complement(2791..3504)
                     /locus_tag="ACHKXP_003748"
     CDS             complement(2791..3504)
                     /locus_tag="ACHKXP_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO7648674.1"
                     /translation="MTMIEIGSIAIQLNRKAIKNLHISVLPPDGRVRVSAPVSMTETA
                     IRMAVIRRIPWIKQQQNNFIKQPRQSEREMVSGECHYLWGRRYRLNVVTHVGKHEAKL
                     GRGRLHLYVSHTATTEQKALVLTEYYREALKKRIADLLPIWESKIGVTVDAWGVRKMK
                     TKWGSCNTPAKRIWLNLELAKKPPECLEYILVHELVHLLERKHNERFKAYMDKLLPDW
                     QERKDLLNKAPLAYSHWLY"
     gene            complement(3504..6596)
                     /locus_tag="ACHKXP_003749"
     CDS             complement(3504..6596)
                     /locus_tag="ACHKXP_003749"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFO7648675.1"
                     /translation="MSNVGQRERATQNRIVQFFQTDLGYRYLGDWQDRANNKNIEVDI
                     LVNWLKKRGVSEALINRAIRQLDTAAALGEGKKLYYANKEVYRLLRYGVKEKEGAGHL
                     NETVWLIDWKNPEANDFAIAEEVSIKGENKKRPDIVLYVNGIALGVIELKRSSVSVSE
                     GIRQNLDNQKKDFIRNFFTTMQLVMAGNDTQGLRYGTIETPEKHYLEWKEDCTNPYEH
                     KLDFHLSRVCNKARLLQIIHDFIVFDAGIKKTCRHNQFFGIEAAKRHIARREGGIIWH
                     TQGSGKSLTMVWLAKWIRENVKDARVLIVTDRTELDEQIEKVFTGVEEDIYRTKSGAD
                     LVATLNQPNPWLVCSLVHKFGRQSDSENDTATDEFIAELKKSLPSDFKAKGDLFVFVD
                     ECHRTQSGKLHEAMKSILPEAMFVGFTGTPLMKKDKKKSVEVFGPYIHTYKFDEAVAD
                     GVVLDLRYEARDIDQNVTSQKKIDEWFEAKTRGLSRLAKTQLKQKWGTMQKVLSSKSR
                     LQQIVNDILLDMDTKPRLMDGYGNAMLVCSSVYQACKAYEMFSQTDLAGKVAIVTSFQ
                     PTAASIKGEETGEGLTEKLFKYDIYRKMLADYFEQPEDKAANRVEEFEKEVKKQFIDE
                     PGQMRLLIVVDKLLTGFDAPSATYLYIDKQMADHNLFQAICRVNRLDGDDKEYGYIID
                     YKDLFRSLDKAISDYTQGAFDGYDKEDVAGLLKDRLEQAKLDLDNALEMVRALCEPVK
                     APRNTEDYIYYFCGKSGLNQDELTEKEALRLTLYQNVAKLLRAFANIANEMPDAGYSA
                     QDVDLIRAEVAHFEKVRDEVKLASGDLVEMKRFEPAMRHLLDMYIRADDSEVLMDFEE
                     LGLIELIVEKGADAVEALPESIRKNQEAMAETIENNVRKIIVDENPVNPKYYEQMSVL
                     LNELIELRRQKAIEYQDYLEKIRELAAKVVRSEQTASNYPASMDTNPKRAFYDNFGKD
                     EWLATKIDSAIRYTKKADWVGDRFKEREIANAIREETADYELDIQAVMELAKAQKEYQ
                     "
     gene            complement(6589..8625)
                     /locus_tag="ACHKXP_003750"
     CDS             complement(6589..8625)
                     /locus_tag="ACHKXP_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO7648676.1"
                     /translation="MSNVRMPYTKYNDGADGKAIASFKVKKLLCDASQYVVPMYQRNY
                     AWGEGEINQLIQDVLDYQHSKPDQTYYIGTLVVFERNDGSFEVIDGQQRFTTLTLLTF
                     ALKRLYAAGKVTVDMGWYTTPNLNFESRQKSSDTFACLIQGVALEHLKTDDYNLDVVN
                     GFSLLEKGLLALGDKLEGFCQYLYEKVEITRVSVPKDTDLNHYFEVMNNRGEQLEKHE
                     VVKARLMSVLNEITDPYEREQSIQLLGKVWDATANMERYVQYGFNPYERHNIFGKDWG
                     QFKPTCYADLATSICEATTQKSENTQTISLTLNEILAKPPQSSSSADIMFTPERFNSV
                     INFSNFLLHVLRVLTKQDIALDDKRLLEQFDAYLKENGSNKINAVKDFVFALLKCKYL
                     FDQYIIKREFSDGKDRWSLKKLHFYNAKSQSYINTFGGSEEQDNEDGFEGINRQILML
                     LSALHVSTPTLVYKHWLNGALFHLFAMDEITPRLYLQKLEYLSRQFVYGRFLSASPAD
                     YYQMIFQNKEYSAFDPNNQQVLVKLRYGVIENNLVFNYLDYLLWCEGKLNKTDDVITQ
                     FEFTFRSSVEHFYPQHPLDGHHVLPDADLHRFGNLCLISHSKNSKLSNLQPTAKRDHF
                     KAAIADKSIDTLKLYEMIKLMNADGEWTKIQIATHEQAMLMVFFKDSNKGFSYE"
     gene            complement(8618..10084)
                     /locus_tag="ACHKXP_003751"
     CDS             complement(8618..10084)
                     /locus_tag="ACHKXP_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFO7648677.1"
                     /translation="MAFVAEQLKQKNTLDKQVIQVKTLVSLPNISIPAYQRPYKWTHA
                     NLVDLLSDLKVYRDKSAYRLGSVVFHRHSDSESKLKTLDIVDGQQRTLTLVLLVKALL
                     DERLDDLKRQDVKDTLASLAVPIDAFLNRQTFNSDISHRNLHQNFMAAKRAVARSDFT
                     EADIDFLLNRCEVVTFVLDDVSEAFQFFDSQNARGRDLDPHDLLKAFHLREFSESETE
                     LKADSVRHWESLESEQLARLFSNYLFRIRNWAQGKSAQYFTKSKVGLFKGVNLDQVGL
                     YPYVEPLRIAHHFVDDYNGQYQRHIDHQHMSFPFHLDQMIINGRRFFEMTSHYQQKIA
                     EIVSDERSRKDKAKVSEEKQSIRILGHELEPLACKILHTINTYRSRTRTGDGYVRTLF
                     DCAVIFYLDKFGAHQLSQAIEKLFIWAYSCRLEMQVVGIERMDKHALTHNVFERIKQA
                     VQPADVLNWPLATLKKSDCKGTNLSEITELFKEMKYYE"
     gene            complement(10097..10627)
                     /locus_tag="ACHKXP_003752"
     CDS             complement(10097..10627)
                     /locus_tag="ACHKXP_003752"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7648678.1"
                     /translation="MNGRAYSLHEWENSGTPVIRLQNLTGRGDEYYYSNLQLPEKQYC
                     KYGDLLFMWSATFGPVIWRGPKAIYHYHIWKIACEVGYSQSYLFYLLDDMTEKLKRSS
                     SSGGTMLHVTKEKMESTKAAFPSYEEQTAIATILSDMDAEIQALEQRLGKTRQIKQGM
                     MQELLTGKTRLPYDKE"
     gene            complement(10703..11473)
                     /gene="yafV"
                     /locus_tag="ACHKXP_003753"
     CDS             complement(10703..11473)
                     /gene="yafV"
                     /locus_tag="ACHKXP_003753"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO7648679.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYREKFPAWRDADEFRLW"
     gene            11654..12100
                     /gene="ivy"
                     /locus_tag="ACHKXP_003754"
     CDS             11654..12100
                     /gene="ivy"
                     /locus_tag="ACHKXP_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO7648680.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(12143..14587)
                     /gene="fadE"
                     /locus_tag="ACHKXP_003755"
     CDS             complement(12143..14587)
                     /gene="fadE"
                     /locus_tag="ACHKXP_003755"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO7648681.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            14827..15405
                     /gene="lpcA"
                     /locus_tag="ACHKXP_003756"
     CDS             14827..15405
                     /gene="lpcA"
                     /locus_tag="ACHKXP_003756"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO7648682.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            15611..16378
                     /gene="yafJ"
                     /locus_tag="ACHKXP_003757"
     CDS             15611..16378
                     /gene="yafJ"
                     /locus_tag="ACHKXP_003757"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO7648683.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(16349..17089)
                     /gene="dpaA"
                     /locus_tag="ACHKXP_003758"
     CDS             complement(16349..17089)
                     /gene="dpaA"
                     /locus_tag="ACHKXP_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO7648684.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(17245..17523)
                     /gene="yafQ"
                     /locus_tag="ACHKXP_003759"
     CDS             complement(17245..17523)
                     /gene="yafQ"
                     /locus_tag="ACHKXP_003759"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO7648685.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAVLFG"
     gene            complement(17526..17786)
                     /gene="dinJ"
                     /locus_tag="ACHKXP_003760"
     CDS             complement(17526..17786)
                     /gene="dinJ"
                     /locus_tag="ACHKXP_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO7648686.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKNADDLFDKLGI"
     gene            17996..18745
                     /gene="yafL"
                     /locus_tag="ACHKXP_003761"
     CDS             17996..18745
                     /gene="yafL"
                     /locus_tag="ACHKXP_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7648687.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSEHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRISRLAEPFWQDHFLGARRILTEETIL"
     gene            18921..>19127
                     /locus_tag="ACHKXP_003762"
     CDS             18921..>19127
                     /locus_tag="ACHKXP_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7648688.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTIQYQMLRNAIIKVKR
                     DRPFEINAWVILPEHMHCIWTLPES"
BASE COUNT         4984 a         4626 c         4273 g         5244 t
ORIGIN      
        1 gtaatcgatc aactgttgtt ttggtgctta aataagaaaa agagttgatg agaaagtgag
       61 tcaaaatgga agggaataaa atgatgttta aagaatgctc tcctttattg aataggttgg
      121 tatgctcgat attatcaaat accaaaatac gggataagtc tttatgttaa ccctaacgct
      181 ttataaacaa cgtcgtttac ccatattgtc gatatgaaaa ccgaactccg ttaagaccca
      241 atggtttgat gtattccatc gaatcatttc ttttttctcc agcgagttga gcgtcctttt
      301 gttgcagagt gagccataat tgagtttcac attactcaag gtttttagtt gacgttctga
      361 tagtttcatc gcgttcaccg taagattcga ttatttaatg tttactgtag ctatagtcgt
      421 tcgcgtccag aaaagtgtgc atgcatttgc gctgtcagta cccataacgc tttatttaat
      481 ttaatatcgc catcaatccc attcactgaa cgcgtgcgag cgcgtttacc tttagcattc
      541 ctaccggata atcccccttt aattaaattt tcttgcaaac gttggtacac cgtccacaaa
      601 tcatctttct tatcctccca acgacgaggt tgtaatactt gttcttctgt aatcggttga
      661 tgctcctcac caaagcgata cgtcaaagct gcttgtgcta aggctttctg tgctggtggc
      721 ggtaatacca agctttgcat ttcttcgcgt ttatctgcga ttgcatcaaa cgtccctaat
      781 acttcgtaag ccccttcaat caccttgcac actacatcac ctttgtgcgg cacacgcact
      841 tcaccaaagg tatcgccaca aacgagtcca ttttgacaaa cataacgaaa aaagcccggt
      901 aacatctgat agctactgga gccatcatgg ctattaagta agatgatttc aggcacttga
      961 ctacccgtaa tttggtcatg acgtcggagt cgtaacatat gcttagtatg atcacgacgg
     1021 ctgtcatctc gcacacgagt ttggcaggca aagaacggat aaaagccttc tttttgcagg
     1081 ctatccaata aggtaatcgt aggaatataa gtatactttt cgctacgaga ttcatgcttt
     1141 tcttcagaga acacactggg tacggtgcga aataactctt cgagagttaa cgggcggtca
     1201 cggcgaatgc tattcgccgc accaaagcga gacgctaagc gagtcataat gaattccctt
     1261 taaaataaag tagatagata atacgaaggc gtaaataaga acgtggttaa tcgattagat
     1321 ggaaaatggc ggaacgttct gggtgttgcg ccgcataatc acgcaattga taaaaatgat
     1381 cgacgaggat gtcactttcc gtttgaaatg accataagct gtacatcatc aaacacacgg
     1441 caatgcctgc ggcttcacaa ctgacgttgg cttcgttacc gttatgtggg ttgaacagcg
     1501 tcagtgtctc ttgaggtaaa tcgggataaa taaaagcgcc accattggat aaggtgcaat
     1561 attcccaata tccaccctga tagtcatcac aaaactgccc catagtggta aagatgacca
     1621 cttcaaaggt gctccagcct ttcacgcttc cgaagtgatt gagccaaaaa tcaaggcgtt
     1681 gctcatcggg gacgagtgtc attgcaatag cagattcagt tgtgttattc attagttgat
     1741 actccaagaa aaagagataa aaaagcgcca atcctgttaa ggattgacgc tgagagtgat
     1801 aattaatggg gtaatgtaag aggttaaaag aacattctcc acagggattt agcgacggat
     1861 gcgatactgt ttttaacggt gctaaccgta cttcttatca atggtggcaa gggaatgata
     1921 tcgattaagg tatccacaat atcactgaca cactgaccaa aatcgttacg tgcggcgttc
     1981 tccaccgatt gcgtccggta agggatgttc agttgcctag ctactccgct actggctttc
     2041 gccggtaacg cctgtatgag ttgctctgct aattcagtga gttgatagtt ttcgaccgca
     2101 gaaactggta tcacaggatg atgtggctta aaggcggtta tgacggcttg ttgctttaat
     2161 tttaagttat aggcctgctc tgaggatggc ttgtggttgt gttcattcca ctggcgacaa
     2221 ggctctattt tatcggcttg gttcaagaca aatagaaatt gattaggttg gtaaccgcat
     2281 ttcttcgtga gaaagcgata acactgttca tcagaagacc atgctcggtc atcggccttg
     2341 agtacccaaa tgattaaatc gagttctggt aataagttgc gatagagctg gtggtactct
     2401 ttatcccgct caaggctttc ccccacgccg ggtaaatcta caaaagtgag cgtatggttg
     2461 ttcatcgtca tgctgaagcg ttgggcttgg cgtgtgcagc ctgaaacatc actcacaggt
     2521 gataatgacg attggaatag tgcgttgata agactagatt ttcccgctcc cgttttaccc
     2581 atcaaaccga tgatgggaga gtagttgatt aggtggctga gttggttgaa aaaagaggtt
     2641 tggaatgaag tcggtagagt cgataggagg gttttaaagt gagcgttgtg atgcatatta
     2701 ctccttgaaa taaaagaaaa tgatagttat tcaaggagat aatatatatc tgagattttt
     2761 tataatactg agtagagttt cgaattaaag ttagtacaac caatggctgt aagccaacgg
     2821 tgctttattc agcaagtctt tgcgttcttg ccaatcagga agtagcttat ccatataggc
     2881 tttaaatcgt tcattatgtt tacgttcaag taggtgaact aactcatgaa ctaggatgta
     2941 ttctaaacat tctggtggtt ttttggcgag ttctaagttt aaccaaatac gttttgctgg
     3001 cgtattgcaa ctgccccatt tggttttcat ttttctcacg ccccaagcgt cgacagtgac
     3061 acctattttg ctttcccaaa taggtaacaa gtcagctatt cgctttttaa gtgcttcacg
     3121 gtagtattca gtgagaacta aagccttttg ctcagtagtt gcagtatggc ttacatacaa
     3181 atgtaatcgc ccacggccta atttggcttc atgctttcct acatgtgtta caacattgag
     3241 gcgatagcgc cgaccccaca agtaatggca ttctccactg accatttcgc gttctgattg
     3301 gcggggttgt tttataaaat tattctgctg ctgtttaatc caagggatac gacggatcac
     3361 cgccatacga atagctgttt ctgtcataga tacaggtgca gatacacgta cacgaccatc
     3421 tggtggtaac acgctgatat gtaaattttt gattgcttta cgattcaact gtattgcgat
     3481 tgaaccaatt tcaatcatcg tcatcattga tactcttttt gagctttagc gagttccatt
     3541 acagcctgaa tatccaattc ataatcagct gtttcctctc gaatggcatt ggcaatttcc
     3601 cgttctttaa aacgatctcc aacccagtcg gccttcttcg tataacgaat tgcactatca
     3661 attttggtgg caagccattc atctttgcca aagttatcat aaaaagcacg tttgggatta
     3721 gtatccattg atgcagggta attagatgct gtttgttcag aacgtaccac tttagcagcg
     3781 agttcgcgaa ttttctcgag gtaatcttga tattcaatgg ctttttgtcg acgaagttcg
     3841 atcagttcat tcagtaatac tgacatctgt tcatagtatt tagggttaac cggattttca
     3901 tcgacaataa tttttcgaac gttattttcg atggtttcgg ccatggcttc ctgattctta
     3961 cgaatacttt caggcaaagc ctctacggca tcagcacctt tttcgacaat gagctcaatt
     4021 agccctagct cttcaaaatc catcagtact tcgctgtcat ccgcacgaat atacatatct
     4081 aacaaatggc gcatggcagg ctcaaagcgt ttcatttcta ccaaatcgcc actggccagt
     4141 tttacttcgt cacggacttt ttcaaaatga gcaacttctg ctcttatcaa gtctacatct
     4201 tgcgcagaat agcctgcatc aggcatttca ttggcgatat tggcgaacgc acgcagcagt
     4261 ttggcaacgt tctgataaag cgttaaacgc agtgcttctt tctcggtgag ctcgtcttgg
     4321 ttcagacctg acttaccaca aaaataataa atgtagtctt ccgtattgcg tggcgctttt
     4381 accggctcac acaaggcgcg caccatttcc agcgcattat ccaaatcgag cttagcttgc
     4441 tctaaacgat ctttcagcag ccctgcaacg tcttccttgt cataaccatc aaatgcgcct
     4501 tgagtataat cagaaatcgc tttatccaaa gagcggaaca gatctttgta atcaatgatg
     4561 taaccgtatt ctttatcatc gccatccaag cggttcacgc ggcaaatggc ctgaaacagg
     4621 ttgtgatcag ccatctgctt gtcgatatac aaataggtag ccgaaggggc gtcgaagcca
     4681 gtcagcaact tatcgaccac gatcaataag cgcatttgcc ctggctcatc aataaattgt
     4741 tttttaactt ctttttcaaa ctcttcaaca cggtttgcgg ccttatcttc cggttgctcg
     4801 aagtaatccg caagcatctt gcggtaaata tcgtacttaa agagcttttc cgttaagcct
     4861 tcaccggttt cttccccttt aatgctcgca gcagtcggct gaaaactggt gacaatagcc
     4921 accttaccgg ctaaatctgt ttggctgaac atttcataag ctttacaagc ttgatagaca
     4981 ctagagcaga ccaacatggc attgccgtag ccatccatca ggcgtggctt agtgtccata
     5041 tccagcaaga tatcgttcac aatttgctgc aaacgcgatt tactggacag caccttttgc
     5101 atggtgcccc atttctgttt caactgggtt ttcgctaatc gagataagcc gcgagttttg
     5161 gcttcaaacc attcatcgat ctttttttgc gacgtgacat tttggtcgat gtctcgtgct
     5221 tcgtagcgta aatcgagcac aacgccatcg gccacagctt catcaaactt ataggtgtga
     5281 atgtaagggc caaatacttc aaccgatttt ttcttgtctt tcttcatcaa gggggtgccg
     5341 gtgaagccaa caaacatggc ttctggcaga atggacttca tcgcttcgtg caacttgccc
     5401 gattgagtac ggtggcattc atcaacaaat acgaataggt cgcctttggc tttaaagtcc
     5461 gatggtaggg atttctttaa ctcggcaata aactcatccg tcgcggtatc gttttccgaa
     5521 tcagactgcc gaccaaattt atggactaaa gagcatacca gccaagggtt gggctgattg
     5581 agcgtcgcca ccaaatcagc accgcttttg gtgcgataaa tatcctcctc gacaccggtg
     5641 aacacttttt caatctgttc gtccagctca gtacggtcag tcacgatcag cacacgagcg
     5701 tctttgacat tctcacgtat ccatttggcc agccacacca tagtgaggct tttgccagaa
     5761 ccttgcgtat gccaaataat gccgccttcc cgccttgcga tatggcgttt tgccgcttca
     5821 ataccaaaga attgattgtg gcgacaggtc ttttttatac ccgcatcgaa aacaataaaa
     5881 tcatggataa tttgcagcaa gcgcgctttg ttacacacgc gactcaggtg aaaatcgagt
     5941 ttatgttcgt atgggttggt gcaatcttcc ttccactcaa ggtagtgctt ttctggtgtc
     6001 tcaatcgtgc cgtaacgcaa gccttgcgta tcgttacccg ccatcaccag ttgcatggtg
     6061 gtaaagaagt tacggataaa atctttcttc tgattatcga ggttctggcg aatcccttca
     6121 gagacagaca ccgaagagcg tttcagctca atcacgccca gagcaatacc gttaacatac
     6181 agcactatgt ctgggcgttt tttgttctcg cccttaatgg acacttcttc ggcaatggca
     6241 aaatcgtttg cttctgggtt tttccagtca atcagccaca cggtttcatt aagatggcca
     6301 gcaccttctt tttctttcac gccgtaacgc agcaggcgat aaacctcttt gttggcgtaa
     6361 tagagctttt tgccttcgcc cagtgccgct gccgtatcca attggcgaat agcacgatta
     6421 atcaacgcct cgctgacacc gcgttttttt agccaattaa cgaggatatc cacttcaata
     6481 tttttattat tagcgcgatc ctgccaatcg cctaaatagc gatagcctaa gtcagtttga
     6541 aaaaactgaa cgatacgatt ttgggtagca cgttcgcgtt gaccgacatt actcatagct
     6601 aaaaccctta ttcgagtcct taaaaaacac cattaacatg gcttgttcat gcgtagctat
     6661 ttgaattttt gtccattctc catcagcatt catcaactta atcatttcat acagttttag
     6721 ggtatcaata cttttatctg cgatggccgc tttaaaatga tcacgcttag cggtgggttg
     6781 taagttactt aattttgaat ttttgctatg gctaatcaag cacaaattac caaagcgatg
     6841 caaatcagca tccggcagta catgatgccc atcaagtggg tgctgcggat aaaagtgttc
     6901 aacagagctt cggaaggtaa actcaaactg agtaatcaca tcatccgttt tgtttagttt
     6961 tccctcacac cacaacaagt aatcgagata gttaaacact aagttatttt caattacgcc
     7021 ataacgcagc tttactaata cttgctgatt atttggatca aatgctgaat attctttgtt
     7081 ttgaaaaatc atttggtagt aatcagcagg agatgcactt aaaaaacgac catatacaaa
     7141 ttgacgagat aaatattcaa gcttctgcaa ataaagacga ggcgtaattt catccatagc
     7201 aaacaaatgg aacaatgcac catttaacca gtgcttataa accagtgtcg gtgtggaaac
     7261 atgcaaagca cttaacagca ttaaaatctg acgatttatc ccttcaaagc catcttcatt
     7321 atcctgttct tcgctgccac caaaagtatt aatgtagctc tgactcttag cgttataaaa
     7381 atgcagcttt ttcaagctcc acctatcttt gccatcacta aattcccgct taatgatgta
     7441 ctggtcaaaa agatatttgc acttgagtag cgcgaataca aagtctttaa ctgcgttaat
     7501 tttgttactg ccattttctt tcaagtaggc gtcgaactgc tctaacaaac gcttatcgtc
     7561 caacgcgata tcctgttttg ttaacactcg taaaacatgc agtaagaagt tagagaaatt
     7621 aatgacgcta ttaaagcgct ctggtgtaaa cattatatca gcgctagaag aggactgagg
     7681 tggctttgcc aatatctcat tgagcgtcag cgaaatggtt tgagtgtttt cgctcttttg
     7741 agtagttgct tcgcaaatgc ttgtagctaa atcagcgtag cacgttggtt taaattgtcc
     7801 ccaatcttta ccaaaaatat tatgcctttc ataggggtta aagccgtatt gaacgtaacg
     7861 ttccatatta gctgttgcgt cccacacttt tcctaacagc tgtatactct gctcacgctc
     7921 ataggggtca gtgatttcat ttaacaccga cattaacctt gctttaacga cttcgtgctt
     7981 ttctagctgc tcaccgcgat tattcattac ctcaaaataa tgatttaaat cggtatcttt
     8041 gggcacagat actcgggtta tttctacctt ttcgtaaagg tattggcaaa atccttcaag
     8101 cttatccccc aaagctaata agcccttttc tagcaaagag aaaccattca ccacatcgag
     8161 gttgtagtca tcggttttta agtgttctag tgcaacgcct tgaattaggc aggcaaaggt
     8221 atccgatgat ttttggcggc tttcaaaatt gagattagga gtagtgtacc agcccatatc
     8281 gacagtcact ttgcctgcag catataagcg tttgagagca aaggttaata aggtaagtgt
     8341 ggtaaaacgt tgttgcccat caatcacttc aaagctgcca tcgttacgct caaacacgac
     8401 caacgtgcca atgtagtagg tttgatctgg tttactatgt tgataatcca atacgtcttg
     8461 gatcagttgg ttgatttcac cctcacccca cgcatagttc cgttgataca tagggacgac
     8521 atattggctt gcatcacaaa gtaatttttt aaccttaaag ctggcaattg ctttaccatc
     8581 agccccatca ttgtatttcg tgtatggcat ccgaacatta ctcatagtac ttcatttcct
     8641 taaacagctc ggtaatttca ctaagattgg ttcccttaca atcagacttt ttaagtgtgg
     8701 ctaacggcca atttaaaaca tcagctggtt gcaccgcttg ctttatacgt tcaaagacat
     8761 tatgtgtcag agcgtgttta tccatgcgct ctataccgac gacttgcatt tctaatcggc
     8821 agctatatgc ccaaataaac agcttctcta tcgcttgcga aagctgatgt gcgccaaact
     8881 tatctagata aaagatcacc gcacaatcaa acagtgtgcg cacatagcca tcccctgtgc
     8941 gggttcgaga gcggtaggta tttatggtat gtaaaatctt acaagctaag ggctctagct
     9001 catggccgag tatgcgaatg ctctgttttt cttcactaac cttggcctta tcttttctac
     9061 ttcgttcatc gcttacgatc tcagctattt tttgttggta atggctggtc atttcaaaaa
     9121 aacgcctacc atttatgatc atctgatcaa ggtgaaaagg gaaactcatg tgttggtgat
     9181 caatatggcg ctggtactgg ccgttatagt catcaacaaa atggtgagcg attcgaagcg
     9241 gctccacata agggtaaagc cctacctgat caagattgac tcctttaaat aaacccactt
     9301 ttgatttagt aaaatactgt gccgatttac cttgtgccca gttgcgaata cgaaacaaat
     9361 agttggaaaa cagtcgcgcc aactgttcac tttccaggct ttcccaatgg cgcaccgagt
     9421 cagctttaag ttcagtttcg ctttctgaaa actcacgcag atgaaacgct tttaacaaat
     9481 catgaggatc taaatctcgg cctctggcgt tttgcgagtc aaaaaactgg aaagcctctg
     9541 acacatcatc aagcacaaag gtaaccactt cacagcggtt tagtaaaaaa tcgatatcgg
     9601 cctcggtaaa gtcactgcga gcaacagcac gttttgccgc cataaagttt tgatgcaaat
     9661 ttcggtgcga aatatcacta ttaaaggttt gtcgatttaa aaatgcatca atcggcacag
     9721 caagtgaggc gagtgtgtct ttaacatctt gccgctttag gtcatccaag cgctcatcta
     9781 acaacgcctt aaccagaagt actaaggtta aagtgcgctg ttgtccgtca acaatatcga
     9841 gcgtttttag tttactttct gagtctgagt gcctatgaaa caccacagaa cccaagcgat
     9901 aagccgattt atcccgatat acttttaagt cgctcaacaa gtcaactaga tttgcatgtg
     9961 tccatttata aggccgttga taggctggaa tagagatgtt cggtaaagaa actaacgtct
    10021 tcacctgtat tacttgttta tccagcgtat ttttttgttt tagttgctcc gcaacaaagg
    10081 ccatagcagc cactccttat tccttgtcat aaggcagccg agtcttaccc gtcagcagct
    10141 cttgcatcat gccttgtttg atttggcggg ttttgccaag gcgttgttcc agtgcctgaa
    10201 tctctgcgtc catatcggag aggatggtgg caatggcagt ttgttcttca tatgaaggaa
    10261 aagcagcctt ggtggactcc attttttctt ttgtgacatg cagcattgtt cctccactag
    10321 aggagctacg ctttaacttc tcagtcatat catcaagcaa atagaacaag taactctggc
    10381 tataaccaac ttcacaagca attttccaaa tatgatagtg ataaatcgcc ttaggtcctc
    10441 gccatatcac aggaccaaaa gttgctgacc acatgaataa caaatcaccg tatttacagt
    10501 attgcttttc aggcaactgt aaatttgaat agtaatactc atcccctcga cctgttaaat
    10561 tttgcaatct aataactggg gttccactat tttcccactc atgcaacgaa tacgctctac
    10621 cattaataaa tgagctagca tcacctcgct cagcatacta aacatccctt tctgaattcc
    10681 ccgccctaaa cacaacaccc actcaccaca acctaaactc atccgcatcc cgccatgccg
    10741 gaaacttttc gcgatattcc cttaacaccg ccatcgacag ctccgcatca atgcgcgttg
    10801 cctgatgcgc gtcggcagta gcgataatct cgccttgtgg attaatcacc cggctgtcac
    10861 cgcgataatg gcagccgttg ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct
    10921 gattctcaat cgcgcgggcc gtcagcaatg cctgccagtg cagagagcgc ggagcaggcc
    10981 agttggcgac gtacagggcg aggtcataat cgttgagatt gcgcgaccac acaggaaaac
    11041 gtaagtcgta gcacaccagc ggcaaaatac gccagccgcg ccattccaca atcactcgcg
    11101 cattgcctgc tttgtaatgc agatgctcat ctgccatgcg gaacagatga cgcttatcat
    11161 aaaaatgtac cgtgccgccc ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg
    11221 tttgtaatgc aacactgcct gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc
    11281 agttcactac gtcatcttgt gctagcgacg aagctgccgc ttccatggca aagccgctgg
    11341 taaacatctc cggtagaacg atcacatcgc gcccggtaat accttccagt tgacgatcaa
    11401 aatgacgcag gttggcagga ccatccatcc acaccagtgg ttgctgcaaa agcgtaatct
    11461 tcaaaccagg cacggtgtac aactccttta tgcgaagggt tttataactt taacacctta
    11521 tcaggcagtt gccttagcgc agaataaatt gataacaaat gctgatattg gaaatatctg
    11581 atttgcaaat tatcgtgtta tcgccaagct ttaggaggtt aataacatgg gcaggataag
    11641 ctcgggagga atgatgttta aggcaataac gacagtcgcc gcactggtca tcgccaccag
    11701 tgcaatggcg caggatgatt taaccattag cagccttgca aagggcgaaa ccaccaaagc
    11761 tgcatttaat cagatggtgc aagggcataa gctgcctgcc tgggtgatga aaggcggtac
    11821 ttatacccct gcacaaaccg taacgttggg agatgagacg tatcaggtga tgagcgcgtg
    11881 caaaccgcat gactgtggct cgcaacgtat cgctgtgatg tggtccgaga aatctaatca
    11941 gatgacgggg ctgttctcga ctattgatga gaaaacgtcg caagagaaac tgacctggtt
    12001 gaatgtgaat gatgcgcttt cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg
    12061 cagcctggaa aaccatccgg atggctttaa ttttaaataa ttagcggata aagaaacgga
    12121 gcctttcggc tccgttattc aattacgcgg cttcaacttt ccgcactttc tccggcaact
    12181 ttaccggctt cgtcgccagc tcttccggat caaagtcatc aacgttaata ctgcgcagac
    12241 ggctttcttc agctttcacc agaatagcgg cttcatcttt atcaatcagc cccttcgcca
    12301 gcgcgttgtg cgccagttca tccagacggg taaatggcag atttttaccc agctctttac
    12361 agatccgctg atgaatcggg tcggcggcaa tcacatccac caacgcctct tccagcaagc
    12421 caaccggatt atgctcgctc ggcgtcaggt actgaccgcg accaatgcgg gaacgggtgg
    12481 cgttcggcac ttgtaaaatc ttcgccactt tatgatccag cttgtcagac ggtgccagat
    12541 aatgacgtcc ggtcgggaag atcaccacat tcagcagccc ggcaaccacg cggttcggga
    12601 agttttgcag taaatcgtcc atcgcctgtt cagcctgata cagcgcatcc tgtacgcccc
    12661 agtgcaccag cggcaggtcg gcttcattac ggccttcgtc gtcataacgt ttcagaacgg
    12721 cggaggcgag gtagagctgg cttaaaatat cacccagacg ggccgagatg cgctcgcgac
    12781 gtttcaggct gccgcccagc accgccatag acacatcaga aagcagggcg aggttggcgc
    12841 tcaggcggtt caggtgctga tagtagcgtt tagtggcatc gccggttggc gtgctgctgg
    12901 ttaaaccgcg cgtcaggccc agccagaagc tgcgaacttt gttgctaccg acgtgaccga
    12961 tatgtttgaa caacagttta tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct
    13021 cttccagcac gtacggatgg caacgaatcg ctccttgtcc gaagatcatc atgctgcggg
    13081 tcagaatgtt agccccttca acggtgatgg caatcggtgc gccctggtaa gcacgcgcca
    13141 ggaagttgct ttgcccgagc ataatgcctt tgccaccagt gatatccatc gcatcaataa
    13201 tcgactgctg cccgcggtga gtacagtgat acttaacgat agccgacagc acggcaggtt
    13261 tttcgccgag cataatgccg taggtaatca gcgatgccgc agcatccatt acataagcgt
    13321 tcccggcaat acgcgccagc ggctcttcaa tcccttccat cttaccaata gagattttga
    13381 actgacgacg aatgtgcgca tacgcacccg ttgccagcgc tactgatttc acgccgccgg
    13441 ttgagttgga aggcagggtg atgccgcggc ctaccgagag gcactccacc agcatccgcc
    13501 agccttgccc ggccattttc ggcccgccga taatgtaatc gatcggtacg aagacatctt
    13561 taccgcgcgt cggtccattc tggaacggca cgttcagcgg gaagtggcga cgaccaattt
    13621 ccacgcccgg cgtggtggtt gggatcagcg cacaggtaat acctaaatct tctgcgccac
    13681 ccagcaattt ctccgggtcg gagagtttaa acgccagccc aagcacggtc gcaatcggtg
    13741 ccagcgtaat gtagcgtttg ttccaggtca gacgcatccc cagcacctgc tggccctgcc
    13801 attcgcccat gcagacaatc ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg
    13861 ggctggtcag tgcaaagcag gggatctcct gaccacgcgc cagacgcggc agatagtgat
    13921 ctttctgctc gtcagtgccg taatgttgca acagttcgcc cgggcctaat gagtttggca
    13981 cgccgacggt aatcgccagg atcccgctca cgccggagag tttttgcagc acgcgagact
    14041 gggcataagc cgagaactcc agcccaccgt actctttttt gatgatcatc gcgaagaagc
    14101 gatgctcttt aaggtacgcc cacaactccg gcggcagatc tgccagctca tgagtaatct
    14161 ggaaatcatt cgccatccgg caggcttctt ctaccgggcc gtcgagaaac gcttgctctt
    14221 cggcggtcag gcgcggctgc ggatagttat gcagcttttt ccagtccggc ttgccctgga
    14281 acaagtcgcc ctcccaccag gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca
    14341 tcggcggcat caccttacgg aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca
    14401 taggcgcaaa gttaaatggc acgaggataa tggccagagg caccagtacc cacgccgacc
    14461 acagaccagc aacgccgagg gcggctgtcc aggcgagcaa aatcagactg ctgataaata
    14521 agctcacgcg gtgatagaac aacgcgccga gcaggacaac cgtagcgaga atactcaaaa
    14581 tcatcataac gaaaagcccc ttacttgtag gaggtctgac cacttgtgat gatatggttg
    14641 tagtggatgt aaaaacattt agcaatgtgt ttacaatata attacaacaa agctcacatt
    14701 gttgctgttt ttatccgcac ttcaggtcaa aaagtcttgg tcatcgcacc tgcccatact
    14761 tctcgctttt ggcggtatcc ggtacactgc attttgtcta ttacatttat gctgaaggat
    14821 atcctcatgt accaggatct tattcgtaac gaactgaacg aagcggcgga aacgctggct
    14881 aactttttaa aagatgacgc caatattcac gccattcagc gcgcggcggt cctgttagca
    14941 gacagcttta aagccggtgg caaggtgctt tcctgcggca acggcggctc ccactgcgac
    15001 gccatgcact ttgccgaaga gttgaccggt cgctaccgtg aaaaccgtcc gggctacccg
    15061 gcgattgcta tttctgacgt tagtcatatt tcctgcgtcg gtaacgattt cggtttcaat
    15121 gatattttct cccgctatgt tgaagcggta ggtcgcgaag gcgatgtgct gctggggatc
    15181 tccacctccg gtaactctgc gaacgtgatc aaagcgatcg cggcggcgcg tgagaagggg
    15241 atgaaagtga tcaccctgac cggtaaagac ggcggcaaaa tggctggcac ggcggatatt
    15301 gaaattcgcg ttccgcactt tggttatgcc gaccgcattc aggagatcca cattaaagtg
    15361 atccatatcc tgatccagtt aattgaaaaa gagatggtta agtaagtctg gcgtaggccg
    15421 gataaggcgt ttacgccgca tccggcattt gtgcgctgat gcctgatgcg acgctgacgc
    15481 gtcttatcat gcctacaaat ctgtacccga accgtaggcc gaataatgcg tttacgccgc
    15541 atccgacctg aaaatactta aatcaatctt cgccgggggc catgcgctcc cgctgttgtg
    15601 gaggttaccc atgtgcgaat tgctcgggat gagcgccaac gtccctaccg atatctgctt
    15661 tagtttcacc gggcttgtac agcgtggtgg tggaaccggg ccacataaag atggctgggg
    15721 cattaccttt tacgaaggta aaggctgtcg cacatttaaa gatccacaac ccagctttaa
    15781 ttcccccatc gccaaacttg ttcaggacta cccgataaaa tcatgttcgg tggtggctca
    15841 tattcgccag gccaatcggg gcgaggtggc gctggaaaat actcacccat ttacccgcga
    15901 gttatgggga cgcaactgga cttatgccca taacgggcaa ctaacgggct ataaatcact
    15961 ggaaaccggc aatttccgcc cggtcggtga aaccgatagc gaaaaagcct tctgctggct
    16021 cctgcataaa ttaacgcagc gttacccgcg cacaccgggc aacatggcgg cagtgtttaa
    16081 atatatcgcc tcactggcgg atgaactgcg gcagaagggc gttttcaaca tgctgctttc
    16141 ggacgggcgc tatgtgatgg cgtattgctc gactaattta cactggatca cccgtcgcgc
    16201 gccgtttggc gtggcaacgt tgctggatca ggatgtggaa atcgacttca gctcgcagac
    16261 cacaccgaat gatgtggtca cggtgattgc gacacagccg ctgacgggca atgaaacctg
    16321 gcaaaagatt atgccaggcg aatggcgctt attttgcctc ggggagcgtg tagtttgatg
    16381 ccagttgtgg ctgcacaact tcgtggctta acggcttgct gaccacgtaa cgaccattga
    16441 ccacagaaac ggttggtggc ttacgggttt gctcaaagta gtcgtaaccc ggcttcagtt
    16501 gctcccagaa gtccttaaag ttggaatatt tatggcgctt catattggcg tcggtcatgc
    16561 ggaacgggta aatactcact tgcacgctcg gctgaccaaa caccagtgca ccagtaacga
    16621 actggaagat ctcatcaata ccctgattgg tcatcgcgta gcagccgatg gatacacaat
    16681 cgccgtggat catcaggtat ttcccttcat aaccatgcgc acggtcataa gcattgggga
    16741 atccaatatt aatcgctttg tagtaacggc tgtctggttt taactgatta cgctggacgc
    16801 tataaaaccc ttccgggctt ttgaaatcac cctgacgctg ttttggccct aatccgccgg
    16861 aatatttaca gattttatag ctgtcgagca gctgatattg ctcgcccatt ttgacgtaga
    16921 gatcgagcgt acgttcttcc ttgaagatct ggatgtagac aggggatccc atcaactgct
    16981 gcttatactc tttgctcact ggcgtggtcg agctactgct gcccagcaaa ccggcaaacg
    17041 aaacgcacgg gatcaacaac atcgcaagaa ttaatgcgat tttacgcata ctgcttattc
    17101 cttgataaaa cggttacaca cgccaggacg gcaaaatgga tcccaaatcg gaatagtctg
    17161 gatttggaag gctcacatta tcaccaaaag agttttacgc aagcctgtcg gcgctgggtt
    17221 tacaaatttc atcggcagcg ggctttaccc aaatagcacc gcgtgagttc cagttctctc
    17281 aaatcgtaaa agtttatcgg taagtttgta aatcaggatc cagtccggtt cgacatgagc
    17341 atcgcgataa cctttccatg aaccttgtag cgggtggtct ttataaacag ctggaagcgg
    17401 taaagcatta tttataagaa gcgtcataag atatttcaat ttattcatat ccttatgacg
    17461 cttttgtgca agttttacat cctttgaaaa ttgtcccgag tattcaatat ccctttgaat
    17521 catatttaaa ttcctaattt atcaaataaa tcatcggcgt ttttggcctt atgaacatca
    17581 acgccagctt cgctgttttt gattgattga atggttaatt gattaggctc gcgtaaatca
    17641 aacggcaatg ccttttcacg cgcgaccttt gtgagggtta tgcgaaccag gtcagagatg
    17701 gtcagcccca tcccggccag tacgtccgcc gcctgattct tcagatcttc atcgattcgg
    17761 gcgcgaacaa acgcgttagc agccattggg gtatctcctg tgctgtatat atattcagct
    17821 tgaattgtag ctcaattgag caacagcggc aattcatgaa caattcaata aactgatgta
    17881 tttacataat tcactgaaat atatctgtta attttataat aatggtttat taagtttgtt
    17941 cagaaacgat ccgggataca ctgcaacatt aaagcatgac cagccattat tgagtatgtc
    18001 cttgccgtcg attccatcct ttgtattgtc gggattactg ttgatttgtt tgccgttttc
    18061 ttcatttgcc agcgccacca catcacatat ctctttcagc tacgccgccc gccagcggat
    18121 gcaaaaccgt gcgcgtttat taaaacagta ccaaacccat ctgaaaaaac aggccagcta
    18181 tattgtggaa ggcaatgccg aaagcagaag ggcgctgcgc cagcacaacc gcgaacagat
    18241 aaaacagcat ccagaatggt ttcctgcgcc gctcaaggcg agtgacagac gctggcaggc
    18301 gctggcggag aataatcatt tcttaagcag cgagcatctg cataacatca ccgaagtggc
    18361 gattcaccgc ctggagcagc agcttggcaa gccttacgtc tggggcggca cgcggcccga
    18421 tcaaggcttc gactgtagcg ggttggtttt ttatgcctac aacaagatcc tcgaggcgaa
    18481 gctcccacgt acggccaatg agatgtatca ctatcgccgg gcaacgattg tggcgaacaa
    18541 cgaccttcgc cggggagatt tgctgttttt ccatatccac agccgcgaga ttgccgacca
    18601 tatgggcgtg tatttgggcg atgggcaatt tatcgagtcg ccacgtaccg gcgaaaccat
    18661 tcggataagc cgattagccg aacctttctg gcaggaccat tttttgggtg cgcgacggat
    18721 tttgacggaa gagacgattt tgtaggccgg ataaggcgtt tacgccgcat ccggtggttg
    18781 tgcaccgatg cctgatgcga cgctggcgcg tcttatcatg cctacagatc tgtacgtttt
    18841 tgcgagccgc tttcccgtta atccatcacc tcattgccga cctttccttt tcttcttatc
    18901 gttgagaaaa aggagtcgcc atgtctgaat atcgtcgtta ttacatcaag gggggaacct
    18961 ggtttttcac ggtgaattta cgaaatcgtc gaagccaact cttgaccatc cagtaccaga
    19021 tgctccgtaa cgccattatt aaagttaagc gagacaggcc ttttgaaatt aacgcctggg
    19081 taattttgcc agagcatatg cattgcatat ggacattacc tgaaagc
//
LOCUS       DAZFZC010000090        19062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFZC010000090 DAZFZC010000000
VERSION     DAZFZC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.091"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHKXP_003763"
     CDS             complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHKXP_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO7648689.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="ACHKXP_003764"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="ACHKXP_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO7648690.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAIRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSTNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHKXP_003765"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHKXP_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO7648691.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHKXP_003766"
     CDS             complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHKXP_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7648692.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHKXP_003767"
     CDS             3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHKXP_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO7648693.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNQDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTVAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHKXP_003768"
     CDS             3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHKXP_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO7648694.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHKXP_003769"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHKXP_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO7648695.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHKXP_003770"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHKXP_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO7648696.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHKXP_003771"
     CDS             5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHKXP_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO7648697.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="ACHKXP_003772"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="ACHKXP_003772"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO7648698.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALSLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEKTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9067..10089)
                     /gene="lgoD"
                     /locus_tag="ACHKXP_003773"
     CDS             complement(9067..10089)
                     /gene="lgoD"
                     /locus_tag="ACHKXP_003773"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO7648699.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIANLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10228..11142
                     /gene="lgoR"
                     /locus_tag="ACHKXP_003774"
     CDS             10228..11142
                     /gene="lgoR"
                     /locus_tag="ACHKXP_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO7648700.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11357..12718
                     /gene="lgoT"
                     /locus_tag="ACHKXP_003775"
     CDS             11357..12718
                     /gene="lgoT"
                     /locus_tag="ACHKXP_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648701.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(12767..14431)
                     /gene="tsr"
                     /locus_tag="ACHKXP_003776"
     CDS             complement(12767..14431)
                     /gene="tsr"
                     /locus_tag="ACHKXP_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO7648702.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQDDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKNVTPATPRKMAVADSGENWETF"
     gene            14808..16958
                     /gene="btsT"
                     /locus_tag="ACHKXP_003777"
     CDS             14808..16958
                     /gene="btsT"
                     /locus_tag="ACHKXP_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO7648703.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            17008..17211
                     /gene="yjiX"
                     /locus_tag="ACHKXP_003778"
     CDS             17008..17211
                     /gene="yjiX"
                     /locus_tag="ACHKXP_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7648704.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            17222..18178
                     /gene="yjiA"
                     /locus_tag="ACHKXP_003779"
     CDS             17222..18178
                     /gene="yjiA"
                     /locus_tag="ACHKXP_003779"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7648705.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(18212..18514)
                     /locus_tag="ACHKXP_003780"
     CDS             complement(18212..18514)
                     /locus_tag="ACHKXP_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO7648706.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(18501..18788)
                     /locus_tag="ACHKXP_003781"
     CDS             complement(18501..18788)
                     /locus_tag="ACHKXP_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFO7648707.1"
                     /translation="MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
     gene            complement(18987..>19062)
                     /locus_tag="ACHKXP_003782"
     CDS             complement(18987..>19062)
                     /locus_tag="ACHKXP_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7648708.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         4655 a         4985 c         4575 g         4847 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttccct gatttttcat ccctgattca aattcattat
       61 catcagcatg atgccagcaa taagcgccag gcacgatggc agcaaaacgt aatgccgcaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tattaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aactaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatgtta tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccata cgggcgacga tcgccgaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcaa
      601 cgaatgcgct aagttctcta ctggcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc gcaatggtat atcggcctga
      721 tggtgccacc attaatgttg gcgctgctga cgcaggaaaa agcattagat gtgtcgccgg
      781 aacatttcca cgttgagttt cacgaaaccg gacgcgccgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ctacattcgc cgcagcagcg aatggaaacg ttaatcagtc
      901 aggcgctggt tccggtagtg caggcattag aagcaacggg agagatcaac ggcaaactta
      961 tctggagcaa taccggttat ttgatcaact ggtatctgac tgagatgaaa cagctgcttg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaatg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtgg tactgcgcga cggcctgctg gtgcgccgca
     1141 cttgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgttcggct ttgattaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccaa cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aacgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactact
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg
     2041 gcgctgatag ttttattact tttcttaagc aatgaagcta tctggttgat gccccatcct
     2101 ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta
     2161 aaatcatgag tttcagtcat tgccggagag gtcagcatgg cgctgatacg ctctgcgtgc
     2221 gtattaccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac
     2281 gtcgccgtag ggcacatgag caattctact gcctgggaat accaggatcg tgacaccatg
     2341 aaaacccagt gaatttcccg atattgtgag attaaagaat aataatgttc acaaacagaa
     2401 cgtggatctt cactttcacc ggctaaatca gcaatgacta atccggaacg acgtaattgt
     2461 aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc
     2521 ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt
     2581 ttgcagcatc ctggcaacat atttcctcca catccactgt ctaaaaatta tgatggttta
     2641 ttcaggatat tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat
     2701 ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt
     2761 cagaaggtta gcatcaaatg ataacctagt gaaatagcct ggagacattt aaaatatcta
     2821 cattaagttg ttagaaatac ttaatgaaaa acagaattct cattcattta atttataatt
     2881 aactgatatc ccttccagta gaatgcaaaa tcacacaatc aatatttatt caataaaatc
     2941 tgatagtttt aacttgtaaa aattaaaaaa cgcgcccagt tatcttttat cctgattcgt
     3001 aaaggctaaa caataaattc attatagtga taatattcat gtgataattt atttgcgacg
     3061 agagataatt cgctttttat caccgagctt ctcccgataa ccaggattaa tcagcgcaat
     3121 tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
     3181 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
     3241 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
     3301 cgttggttga tgagctttcc tcacgactgg gtcgggcgct gggaatggac agcgtcgaaa
     3361 gttctatctc ttcgaacgcc atagtgctga caactattaa agatgggcaa tgcctgacat
     3421 cgacacgaaa aaatcaagat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
     3481 ttgtgattct ggcggagcat catctgctgg attacaaagg tgtagagaaa cgatttagcc
     3541 aaattcagcc attacgttac ccgagatggc tggtagcctt aatggtcggc ctttcttgcg
     3601 cctgtttctg taaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca
     3661 gtacggtcgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacagatca
     3721 acttttgcct caccgctttc gccgccacca ccatttccgg tttgcttttg caacttccca
     3781 ctttcagcaa tacacccacc atcgcgatgg ccgccagcgt tctgctgctg gtgccgggtt
     3841 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
     3901 gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga
     3961 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     4021 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgtgcgggc
     4081 gttacgctgg tgtgcgctgc ttggctcgat aggtcatggt tcccgaatga tcttaatgac
     4141 cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     4201 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggctgttat
     4261 tcctatgttc cctggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca
     4321 gctaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     4381 aattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     4441 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagctgctc
     4801 ccgtagctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact
     4861 ttatccgcct agcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagt gaacctgaca gccaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgctttc aaaacgggtg aagaactact ggcctggcag aaagaacaag gggcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tatgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacacct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc ttcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagaacaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg
     6601 ttggctaaac agcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacagtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc tcactggcgc tgggttcggt ggacgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaggcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcaaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaaa tcgcacggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt agccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ttctcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aaaccgaatc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa gatactgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cagatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctat aagatggcac aactctgggc tccggaactg gcactctcca
     8341 ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcag catgttgaca
     8401 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttata
     8461 aaggcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8581 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaaa aactcttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     8881 tcgggatcgg catggtggaa attaaagttg tggaacgtga agggtaattt ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacat cttctccggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc
     9061 gccttatcag aaagtaatta cacccttaat caactcacga ttgttaatca catcacgctc
     9121 gtaggtttct gccagcgtgg cgaacgggta gcgatgggtt aacatcatgt cggcagtgat
     9181 tttcccttcc gccatcagtc gaccgacttt agcgaaatct tccggcgtgg cgttgcggct
     9241 acccatcatc gtcgtttctt ttttatggaa ttctggatcg gagaactgca actcaccttt
     9301 aaacagaccg acaaatacca ccgtgccgcc gtgacgaatc agattcacgg tgttattcat
     9361 cgcatgttga ttacctgtcg cgtcgatcac tttctgcgcc agagaaccac caaactgcgc
     9421 ccgcagctgc gcgtcaaaat cttcggctga tgggtccagc acgggcaact ccagacgcgt
     9481 tgccacatgt tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc
     9541 tttagcgatt gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc
     9601 gccgggagca atggctgcgc gacgtaccgc atgagcgcta atagcgaaag gttcaatcaa
     9661 tgctgccgcc tgcgggtcaa taccatctgc gggcaaaatg ttcgccaccg gcacgctcaa
     9721 atactcacta aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt
     9781 ggtacgcccg cttttacacg ccgggcattg ctgacaggca acataaggga tcaccgctac
     9841 ttgctgtcca tttttaagat ttgcaatatt tttacccagc ccaacaatct ccccacatat
     9901 ttcatggcct aaaacccgtg gataactaaa aaatggttga tttccacccc aggcatgaat
     9961 atcggtaccg caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat
    10021 aggtatctcg cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat
    10081 cgtagacatg attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat
    10141 ggcagcggaa cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg
    10201 ttttaatgga ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg
    10261 attaaccagg tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa
    10321 agtgcgctgg cggaaatgta caacatcagc cgcactacag tgcgtcatat tctcagccac
    10381 ttacgcgaat gcggcgtctt aacgcaggtt ggaaacgact atgtgattgc gcgtaaacct
    10441 gaccatgatg acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt
    10501 gagcaggctt ttttcacgat gataaaccag cgccagttac gccctgggga aactttttct
    10561 gaactgcaac tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta
    10621 aaattcggcc gttataatct tattcaaagc gaaaagcgcg gccagtggag catgaagcaa
    10681 ttcgatcaat cctacgccga gcagctgttt gaactacgcg aaatgctcga aacacattca
    10741 cttcagcact ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg
    10801 ctcgaacgtc atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa
    10861 cttgacagag attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa
    10921 tctcttgaga tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt
    10981 aaacagcgca atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc
    11041 cgcagtgacc ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca
    11101 atgattcgat ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc
    11161 aaactattag cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc
    11221 tatgctcaca atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt
    11281 taacctgccg ttaatgtcaa ccttgcgtaa tgcacaatta cgtaaacaat taaaattata
    11341 aatacaggag tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac
    11401 tccatcttcg tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg
    11461 aattaaacgc attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta
    11521 tctcgaccgc agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag
    11581 tgccaccgaa atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact
    11641 tccttgcggc ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt
    11701 cttctggtca ctgttccagg ccatgtctgg catggtgcac agctttacgc agttcgtgtt
    11761 ggtgcgtatc ggtatgggga ttggtgaagc gccgatgaac ccatgtggcg taaaagtcat
    11821 taacgactgg ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc
    11881 taccattggc gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg
    11941 gcgtgggatg tttattacca ttggtgtgct ggggattttt ctcgccattg gctggtatat
    12001 gctctatcgc aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc
    12061 aggtagcgtc aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg
    12121 taaccgcaca atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct
    12181 gtatctggcc tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac
    12241 agggttgatg gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta
    12301 cgttactgac tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat
    12361 tattgccggg atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc
    12421 catgacggcg gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc
    12481 ctgctggggc ttaatccacg tcgcagttgc ttctcgcatg acggcgtcgg tgggcagtat
    12541 ccagaacttt gccagcttca tctgcgcctc ttttgcgccg atcattactg gttttattgt
    12601 tgataccacc cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg
    12661 agcactggcg tacatcttcc tggttcgtca gccgatcaac gacccgcgta aagattaaac
    12721 tccctatgcc cgataacatt ttgcttatcg ggcattttta tggcgattaa aacgtttccc
    12781 agttctcccc gctatctgcc actgccattt tacgcggcgt agctggcgtc acgtttttta
    12841 ccacagccga tgtttcacgc tgctgttgct gctgttgctg aatccggaac actgccacag
    12901 cttcggtcag gcgactggcc tgctcttcca gcgcggcggc ggcagcggca gattcttcca
    12961 ccagcgcagc gttctgttga gttacccggt ccatctcagc aaccgctaag ccaacctgat
    13021 cgataccacg gctctgctca tcagaagcag aagcaatttc gcccataatg tccgtcacgc
    13081 gggtcacggc actgacaatc tccgccattg tttcccctgc gctttcgacc agcgtagagc
    13141 caacatctac tttccccacc gagtcttcaa tcaggctttt aatttcacga gccgcctgag
    13201 cgctgcgctg agccagatta cgcacttcac ccgcgaccac cgcaaaaccg cgcccttgct
    13261 cacccgcgcg cgctgcttca accgccgcgt tcaaagccag aatattggtc tggaaggcaa
    13321 tgccgtcaat tacgctgata atatcggcga ttttctgcga actggtggag atatcgcgca
    13381 tggtctgtac tacgttatcc accactttgc cgccgcgttg cgccgtttca gaagcactta
    13441 acgccagatg gctggcctga cgcgcattct cggcgttctg tttcaccgtt gcggtcagtt
    13501 gctccatgct ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga
    13561 gatcgttatt gccggtggcg atttcgctgg caccgctata gatggcattg gccccgttgc
    13621 gcacatcacc gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt
    13681 gccccatctc attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat
    13741 gacgaatgct gtcaatcagg cgattcattg gcgctaccag cgaggcttta ataccgaacc
    13801 agacggcgaa gatgaccgcc agtacgacga tcatcacgcc caccagaatc cacatcgcct
    13861 ggctgtagga ggcattgtta tcgctgacgg cgatatcata gagccgatcg tcttgctcca
    13921 tgtaagccac atactgcttc tcgaaaccgt cctgatatcc ctgggtcggc tgatcaaaga
    13981 actcgttgat tttgcctgca cctaatagtt ggatcagctc cgccagcgca ttgtgataaa
    14041 tatcgtaatt acgtttgatc tctgccgctg cggcggtgct ctgacgcggg tcacgcggca
    14101 acgcttcgta atccgcccag tttttttccg cctgtttcag cgaaatactg gcactctgca
    14161 tcagctcagc aacggttgaa ccgctaccaa tattattctg atccatcatg tagcggatac
    14221 ccgcgcggtt gagggtgtta cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg
    14281 attgctgctg gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc tttaaggcat
    14341 taaagaacag accgcctgat gtcagttgta aaaggccaaa aacggccaaa accagcagta
    14401 agctggtcac aattttgata cgttttaaca tggtttctct ttcctgtgga ccgatgcaag
    14461 attttcggcc tggaaaggaa aaactttatg aatatcccgc ggagattacg ccgagtaatt
    14521 ttattcacac tctgaattta aaaagccatt atttacataa attattcaca atataaatat
    14581 gtgatatgaa tcacatattt atcgtcactt aaacgacgcc tttgccgctc aaccgcaaaa
    14641 ctgaccgctt acatccctaa aataaccact cagttattta ccttacttta cgcgcgcgta
    14701 actctggcaa catcactaca ggatagcggt caatttacct cctcaaacgc aacgcaaacc
    14761 tagaacggct tcggccaact attaatcaat acatgccagg ttttactatg gatactaaaa
    14821 agatattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
    14881 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtat
    14941 cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac
    15001 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
    15061 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg
    15121 tgggtccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg
    15181 gggtcgtgct ggccggtgcg gttcaggact ttatggtgct gtttatctcc tctcgccgta
    15241 atggcgcatc tcttggtgag atgatcaaag aagagatggg accagtaccg gggactatcg
    15301 cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgtcctggcg ctgattgtgg
    15361 ttaaagccct ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg
    15421 cgctgtttat gggtatctac atgcgcttta ttcgtccggg gcgtgtgggt gaagtctctg
    15481 tcattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
    15541 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
    15601 atgcgtttgt ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg
    15661 caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc
    15721 cggaactgaa aatgcctgcc atgacccagt acattgacgg tactggcccg ctgtggaaag
    15781 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
    15841 cgctgatctc ttccggtacg acgccaaaac tgctggctaa tgaaaccgac gcgcgtttca
    15901 tcggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgctgcgt
    15961 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatca
    16021 ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga
    16081 aagacgttac cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag
    16141 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
    16201 gtgcgccgac gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg
    16261 acatgggctt ctggtatcac ttcggtattc tgttcgaagc actgttcatc ctgaccgcgc
    16321 tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
    16381 cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg
    16441 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
    16501 tgtggccgct gttcggtatt tccaaccaga tgctggcagc cgtagcgctg gtactgggca
    16561 ccgttgtgct gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg
    16621 tatggctgct tatctgcacc acctgggcgc tgggcctgaa actgttcagc accaacccgc
    16681 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
    16741 acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
    16801 caggtctgag tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
    16861 cctggcttgc ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa
    16921 tcccggaagg cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc
    16981 ggggctttgt tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat
    17041 ctcggccagg cggcgaagat gttgattggc attccagact acgacaacta cgtcgagcat
    17101 atgaagacca accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc
    17161 cagaacgcac gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag
    17221 catgaacccg attgcagtta ccctactcac cggtttttta ggcgcgggca aaaccaccct
    17281 gctgcgccat attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt
    17341 cggcgaagtc tccgttgatg atcaactgat tggcgaccgc gccacgcaga tcaaaacgct
    17401 gaccaacggc tgcatctgct gttcgcgctc caacgagctg gaagacgcac tactcgacct
    17461 gctggataat ctcgacaagg gcaatattca gttcgaccgt ctggtcattg aatgcaccgg
    17521 catggccgat cccggcccga ttattcagac ctttttctcc catgagattt tatgccagcg
    17581 ctacctgctg gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa
    17641 ccagttcacc atcgcccagt cccaggttgg ctacgccgac cgtattctgc tgacgaaaac
    17701 cgacgtcgca ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc
    17761 accggtctac accgttaccc acggcgatat cgacctgggc cttctgttca acaccaacgg
    17821 ttttatgctg gaagaaaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggacaa
    17881 acaaaacgat atttcgtcga ttgtggtgga actggattac ccggtagata tcagcgaagt
    17941 ttcccgggtg atggaaaacc tgctgctgga gtcggcggat aaactactgc gttacaaagg
    18001 gatgctatgg attgacggcg aacctaaccg cctgctattc cagggcgtcc aacgcctcta
    18061 cagcgccgac tgggaccgcc cgtggggcga tgaaaaaccg catagcacga tggtgtttat
    18121 cgggattcag ttgccggagg aagagattcg ggcagcgttt gcggggttga ggaagtaatt
    18181 ttggatgatg gccgccatgt ggcggcctga attatttttt gttctgatcg cgcagcatcg
    18241 cttcacgtaa aattgcgttc aggcgagttt gatagccttt acctggtcgc ttcaaccatt
    18301 ccatcacatc agcatcaata cgcaccgaag cctgagtttt caatggccga taaaacttac
    18361 cgcgttcggc gttagaccat tgctcatcgc tggaaggtgg gatatcactg taatcaatat
    18421 cttcgtcgga tttattcgcc agcgccttca gttcggcctc atgctgcaca tcgagtgtag
    18481 aggaggtccc tcgtttatgt ttaaccatgc tcatagcgat tcctctcttt acgatctgct
    18541 tttcgtgcac tgatgattcg aataatttca tttccgcttt cgaaacggat ggtatgcgca
    18601 accagaataa ccacgatgcc gtaaaccagg ccgattgtct gccagcgata ctcaccgttt
    18661 tctatgcgtt cttgctgtga caaatgttgt ggatcatcaa acaataacac cgcatcttca
    18721 aagcggatgc catgtttcac ccgattactt ttggccttgt tttcatccca ttcaaactcc
    18781 attggcataa ttgccatccc taattgtata tacaaaaatg tatctacaat tattctgcaa
    18841 ttatagcgtt gatgttggaa atagcatcac gtttcgaata atcaaccatg aaattgacaa
    18901 tgagtaagtt ggacttgatt catccaatag aatttctcgc ctgacatggc acactaaaca
    18961 cctcgctgtc gggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg
    19021 atgaccttgt catgcagctc caccgatttt gagaacgaca gt
//
LOCUS       DAZFZC010000091        19037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFZC010000091 DAZFZC010000000
VERSION     DAZFZC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.092"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1283)
                     /locus_tag="ACHKXP_003783"
     CDS             complement(<1..1283)
                     /locus_tag="ACHKXP_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7648709.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYAT"
     gene            complement(1408..3855)
                     /gene="glgP"
                     /locus_tag="ACHKXP_003784"
     CDS             complement(1408..3855)
                     /gene="glgP"
                     /locus_tag="ACHKXP_003784"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO7648710.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(3874..5307)
                     /gene="glgA"
                     /locus_tag="ACHKXP_003785"
     CDS             complement(3874..5307)
                     /gene="glgA"
                     /locus_tag="ACHKXP_003785"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO7648711.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(5307..6602)
                     /gene="glgC"
                     /locus_tag="ACHKXP_003786"
     CDS             complement(5307..6602)
                     /gene="glgC"
                     /locus_tag="ACHKXP_003786"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO7648712.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(6620..8593)
                     /gene="glgX"
                     /locus_tag="ACHKXP_003787"
     CDS             complement(6620..8593)
                     /gene="glgX"
                     /locus_tag="ACHKXP_003787"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO7648713.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(8590..10776)
                     /gene="glgB"
                     /locus_tag="ACHKXP_003788"
     CDS             complement(8590..10776)
                     /gene="glgB"
                     /locus_tag="ACHKXP_003788"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO7648714.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(11049..12152)
                     /gene="asd"
                     /locus_tag="ACHKXP_003789"
     CDS             complement(11049..12152)
                     /gene="asd"
                     /locus_tag="ACHKXP_003789"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7648715.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            12344..12937
                     /gene="yhgN"
                     /locus_tag="ACHKXP_003790"
     CDS             12344..12937
                     /gene="yhgN"
                     /locus_tag="ACHKXP_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO7648716.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(12994..14334)
                     /gene="gntU"
                     /locus_tag="ACHKXP_003791"
     CDS             complement(12994..14334)
                     /gene="gntU"
                     /locus_tag="ACHKXP_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7648717.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(14338..14865)
                     /gene="gntK"
                     /locus_tag="ACHKXP_003792"
     CDS             complement(14338..14865)
                     /gene="gntK"
                     /locus_tag="ACHKXP_003792"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO7648718.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(15004..15999)
                     /gene="gntR"
                     /locus_tag="ACHKXP_003793"
     CDS             complement(15004..15999)
                     /gene="gntR"
                     /locus_tag="ACHKXP_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO7648719.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(16223..16918)
                     /gene="yhhW"
                     /locus_tag="ACHKXP_003794"
     CDS             complement(16223..16918)
                     /gene="yhhW"
                     /locus_tag="ACHKXP_003794"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO7648720.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(17041..18078)
                     /gene="yhhX"
                     /locus_tag="ACHKXP_003795"
     CDS             complement(17041..18078)
                     /gene="yhhX"
                     /locus_tag="ACHKXP_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7648721.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFSADDSVGV
                     LEYVNDEGVTVREEIKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            18411..18899
                     /gene="yhhY"
                     /locus_tag="ACHKXP_003796"
     CDS             18411..18899
                     /gene="yhhY"
                     /locus_tag="ACHKXP_003796"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7648722.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDYMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSHWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         4725 a         5150 c         4710 g         4452 t
ORIGIN      
        1 tgtgttgcgt aataacggaa ggtgtaacct attgcgttgt ttgtattacg gtattcggca
       61 acgactttaa tcattccctc catcatactt gccacttctg tttcctgcgg cgagatcatg
      121 cggacatttt tcatgacctg ggattgcatc accgtctggc taccagagtc ttccgggcgt
      181 tgccaggtct ggatctcctg atcgttaccg ccaaccgtgc gccagttggt aattgcacca
      241 ctgaagatgt cacgcacttg ttgttcggtc agggaattaa ccgggttatc tgcattgacg
      301 atgaaaacaa acgcttcacg ggcaaaaggc gtgtaaatca aagtgacgcc cgattcctcc
      361 gcgcgttttt tctgcccacc ggaaggttga gccacaaaga taatatcggc attaccttta
      421 acaatcttgt tatatgcttc gggagtacga gagttcgcca gatattccca ttcgtgaaaa
      481 tcttccggca aaacgcttag tgcataaaag gcagaggcat aaatggggta cgccgccgtt
      541 gcgccatcaa gtcgcggcca gttttgcgtg aactgaattt gcggtttacc acgcagtggt
      601 gtcagttgat tattgagttt atcgggtcgc caggcccagg tatcaatatt ttcacgcaca
      661 ccgacgccgg gattaagctg tttatataaa taagcctggt aaccggcgac aaaaccaagc
      721 atgagaagga taaacaagag agaattacgg caacgtaacc cggacgtgcc cgttaaagga
      781 tatctggtcg gccagccgta ccccagcgaa aaactgatat gcgcgccaac aggtataatg
      841 actaaccacc agtactcacc accgactaat gaggctaaca aaacgcccag ccaggggagc
      901 ataagatcag ccaaccaacc cgctcccatc tggcgaaatt gataatctgc cagatgcagc
      961 gcgattatcc agacccccag ggttaataat gcgggtacta aaaaaagaca ttgtcgccag
     1021 acggctattt ctcttttttc tggtgcacta tcgtccgccc acactcgccc cgagaaaaat
     1081 agcattaatg cgcccagcgc cagcataaaa atgttgagca gaaggtaaat tgttggcgtg
     1141 ataaagatat tacaaacttc aataataccg gcaagtccga cacccagcat ggcaataacc
     1201 gccgccaaaa actgcgccag aatggggatg ccgaaaaaag tcattaccag cgaggtcaaa
     1261 atccattttc tgttttgcat tattctttcc attctttctg aatggcgaaa ttatactccc
     1321 cgagtcccct tgccccttct ggacactttt ccgaaatgat ggcgaaaaaa acgggaccct
     1381 ttggccccgt tctatttatt agtgaaatta caatctcacc ggatcaatat gccagatatg
     1441 atcggcgtac tctttgatag tacggtcaga agagaagtag cccatattgg caatgttcag
     1501 catcgctttt gcggtccact cttcctgaag ctcgtagagt tcatcgactt tatcctgaca
     1561 atcgacatag ctgcgataat ccgccagtac ctggtagtga tcgccgaagt tgatcagcga
     1621 atcaaccaga tcgcgatagc gacccggatc ttccggactg aatacaccgc tgccgatttg
     1681 cgtcagcacc tgatgcagct cctcatcttt ctcgtagtat tcacgcggtt tgtagccctg
     1741 acgacgcagt tcttccactt cttccgctgt gttaccaaaa ataaagatat tgtcagcacc
     1801 gacatgatcc agcatctcga cattcgcacc gtccaacgta ccgatagtca gcgcaccgtt
     1861 aagcgcaaac ttcatgttac tggtgccgga agcttccgtc cccgccagcg aaatctgttc
     1921 agacagatcg gccgccggaa tgatcaactg cgccaggcta acgctgtagt tcgggatgaa
     1981 cacgactttc agcttgtcgc caatctgcgg atcgttgttg atcactttcg ctacgtcatt
     2041 gatcaaatga ataatgtgct tcgccatgta ataggccgaa gccgccttac cgccaaaaat
     2101 attcacgcgc ggtacccact tcgcatccgg gtcggccttg atgcggttat agcgggtaat
     2161 cacatgcaac acattcatca attgacgttt gtattcgtga atacgtttga tttgtacatc
     2221 gaacaacgcc tttggattca ccaccacatt cagctgctgg gcgatatact ctgccagacg
     2281 ctttttgttc tccagcttcg cctgatgcac agcgtgatta accattggga aatcacagtg
     2341 ttgttgcagc tcattaagca ggctaaggtc ggtgcgccag ttacggccca ggtgttcgtc
     2401 cagcacggct gaaagcgatg ggttcgctac cgctagccag cgacgcggcg tcacaccgtt
     2461 ggtgacgttg gtgaaacgac ccgggaagat tttcgcaaag tcggcaaaca acgattgcac
     2521 catcagatta gagtgcagtt ccgatacacc gttaactttg tggctcacaa caaccgccag
     2581 ccaggccata cgcacacgac gaccgttgga ttcatcaatg atcgacgccc gtcccagcag
     2641 atcggtatcg ttcggatact gttcctgcaa ggttttcagg aaatagtcgt tgatttcaaa
     2701 gatgatctgc aggtgacgcg gcagaatttt acccagcata tcaaccggcc aggtttccag
     2761 cgcctcgctc atcagcgtgt ggttagtgta ggagaagacc tgacaacaca cctcaaacgc
     2821 gtcgtcccag ctaaattggt gctcatcgat cagcagacgc atcatctcag gaatcgacag
     2881 taccggatgg gtatcattga gatgaatcgc gattttatcc gccaggttat cgtaggtttt
     2941 atgcaactga taatggcggc ttaaaatgtc ctgaatggtc gaggaaacca ggaagtattc
     3001 ctgacgcagg cgcagctcac gcccggagta ggtggagtca tccggataca gtacgcgaga
     3061 tacgttctcg gagtggtttt tatcttccac tgccgcgaag tagtcaccct ggttgaattt
     3121 accgaggtta atttcgctac tggcttgcgc actccacaaa cgcaacgtgt tggtcgcgtc
     3181 ggtatcgtaa ccagggatta tctgatcgta agcgactccc agaatctctt cggtttcaat
     3241 ccagcgcgtt tttttacctt cctgctgaat gcgaccacca aaacggactt tatagcgcgt
     3301 gttgtggcgt ttgaattccc acgggttacc gtattccagc cagtagtctg gcgactcttt
     3361 ctggctaccg ttaacgatgt tctgcttgaa cataccgtag tcatagcgga tgccgtaacc
     3421 gcgccccggc aaccctaacg tcgccagaga atcaaggaag caagccgcca gacgtcccag
     3481 gccaccgtta ccgaggcccg gatcattttc ttcatcaatc agctcttcga gatttaaccc
     3541 catcgcttcc agtgcgccct gtacatcttc gtaaattcct agcgacaaca tggcgttgga
     3601 gagcgtacgg ccaatcaaaa actccatcga caggtagtaa acctgacgag tttcttgcga
     3661 caactgggca cggtttgaac gtaaccagcg ctccacgaga cgatcgcgca cagcaaataa
     3721 cgttgcgttc agccattcat gtttattggc gacgaccggg tcctttccaa tcgtaaacat
     3781 cagcttgtaa gcgatagagt gcttaagagc ttctacgcta agcgtgggcg atgaatatgt
     3841 aaacggagca ttcatatagg cgtttcctga aaactatttc aagcgatagt aaagctcacg
     3901 gtacgacttc gccgcgacct gccagctaaa atccattgcc atagcctgac gttgcacaaa
     3961 ccgccacagt gaaggacggg accacagtac aaaagcacgc cgaatagccc gtaacagcga
     4021 ccaggcatta ctatcttcaa agacaaaccc actggcgacg ccatctgcaa ggttctcaag
     4081 agaacagtca gaaaccgtat cagcaagccc accggtgcgc cgcactaacg gcagcgtacc
     4141 gtacttcaat ccataaagtt gcgttaagcc gcacggttca aaacggctgg gcaccagaat
     4201 gacgtccgcg ccgcccataa tgcgatgcga aaatgcttcg tgatagccaa tctgaacgcc
     4261 cacctgaccg gggtattccg ctgccgccgc aaggaaacct tcctgcagca ccggatcgcc
     4321 cgcgccgagt agcgccagct gcccgccctg ctccagaagg cccggtaagg cttccagcac
     4381 caggtcgaga cctttctggc tggtcagacg gctcaccact gcaaaaagcg gcactttatc
     4441 gtcaacctta agccccattg cgatttgtaa ctggcgctta ttttccgctt tatcttccaa
     4501 cgtatcgcgg gtgtaacgcg aggccaacag taagtccgtc tcaggactcc agattttctc
     4561 gtccacgccg ttcagtacgc cggaaagacg cccttcacgg tgacgctgtt gcaacagacc
     4621 ttccataccg taggcaaact gcggttcggt gatctcgcga gcgtaggttg gactgaccgc
     4681 cgtaatgtga tcggcatagt acagaccggc cttcaggaaa gagatttgtc cgttgaattc
     4741 cagcccatga atattaaaga atgaccatgg caattggatg tcattcatgt gatgtgcata
     4801 aaacatgcct tgataggcca agttgtgcac agtaaacacc gacttcgccg gacgcccgcg
     4861 cgccgccaga tacgcaggcg caaggcctgc atgccagtcg tgcgcatgca ccacatcagg
     4921 acgccagaat gggtcaagcc cgctggccat ttctgcccca acccacccca gcagcgcaaa
     4981 acgcaatacg ttgtcggtat aggcaaataa gttggtatcg tgatacgggc ttcccggacg
     5041 atcatagaga tgcggcgcgt caatcaggta tatgccaacc ccgttgtaat gaccgaacaa
     5101 cagcgtgata tgtccggcga aggtatcacg acgggatact acctgcgcat cggtcacgcc
     5161 acggcgaata tcgggaaatg caggcaacag tacgcgagcg tcaacgccgt ctgcgatttg
     5221 tgctgcgggt aatgccccaa taacatcagc cagaccgccg gttttaagca gcgggaacat
     5281 ctctgaacat acatgtaaaa cctgcattat cgctcctgtt tatgccctaa cttccgtagc
     5341 atttcgcgcg ttaccagcac gatgccttct tctgaacgat agaaacgacg tgcatcttcc
     5401 tctgcgtttt caccaatcac catgccttcc ggaataacac aagcacgatc gatgacgcag
     5461 cggcgcagac ggcacgagcg acctacccat acttccggta acaatacggc ggaatcaatg
     5521 ttgcagaatg aattcacgcg aacgcgcgag aacagaacgg actgcaccac caccgaaccg
     5581 gagatcacac aaccgccgga aaccagtgag ttaagggtca tcccgtggct accggagcga
     5641 tcctgcacga atttcgctgg cggtaatgat tcattgtagg tgcgaattgg ccaattgcga
     5701 tcgtacatat ccagttccgg caccacagag gccagatcga ggttcgcttt ccagtaagct
     5761 tccagcgtac ccacatcgcg ccagtacggc tcggcatccg ggtcggattg tacgcaagag
     5821 agcgggaacg ggtgcgcata ggccagaccg gcttcggtga tcttgggaat caaatctttg
     5881 ccaaagtcgt ggctggagtt ctcatcgcga tcgtcttctt ccagcagttc atacagatag
     5941 tcggcgtcaa agacgtagat acccatactc gccagagatt tgctcggatc gttcggcatt
     6001 gacggcgggt tagcaggttt ttcaacgaat tcgataattt tatcgttctc atcaaccgcc
     6061 ataacgccaa atgcggaggc ttcttcaatc ggtactggca tacaagcaac ggtgcaacgt
     6121 gcgccttttt cgacgtgatc gataagcata cgcgagtagt cttgcttgta gatatggtcg
     6181 cccgccagga tcaccacgta ttccgcttta taacggcgga taatgtcgag gttttgggtg
     6241 accgcatctg cggtgccgcg ataccagttt tcccctttca ttctctgctg tgctggcagc
     6301 agatcgacaa actcgttcat ttcttcattg aagaatgacc agccgcgctg aatgtgctgc
     6361 accagagtgt gggactggta ctgggtgatc acgcccatac gacggatccc ggagttgatg
     6421 cagttagaca gcgcaaagtc gataatgcgg aacttaccgc cgaagtgtac ggccggtttt
     6481 gctcgcttat tggttaaatc cttcaggcgg gtaccacgtc ctcccgccag tatcagggca
     6541 acagatttca atggcagctg gcgcgccaac attaagtgat cgttcttctc taaactaacc
     6601 atgactaact ccttttttat catctctgga acacacacaa tccgtgtgca ggtccctgcc
     6661 agacagccgt aatcactggg ttatcctctc cagcgaatgg gggaatggcg tgccactccc
     6721 cagcaggtaa aacaatctct gttacctcaa gcgtggcgtt aattgcgatc aaaaagcgat
     6781 ccgagagcag aatttgcagc tgtttcggcc cgttttgcca ctcatccgtg cttaaaggtt
     6841 gagcatatcg atttagccaa cggacattgc cgtcgccttc ttcccaccag cgattctcca
     6901 ccaaagcggg aatgcgcttg cgcagatgga ttaacgcggc ggtaaatgcg gttaaaccac
     6961 tgcttgcctg cgaccagtcc aaccaggtta attggttatc ctgacagtag gcattgttat
     7021 tgccatgctg gctgtgacca tgttcgtcac cggccagtaa catcggcgta ccctgggaga
     7081 gcaacaacgt tgttaacagg gcgtgaatgc tgtcgcgccg ccgttcaacc aggtcaagag
     7141 aaccgcctaa cccttcttta ccatgattgt tactgtaatt gttgttggtc ccgtcgcgat
     7201 tttcttctcc gtttgcttca ttgtgtttat ggttgaagca aacgcagtcg cgaagcgtaa
     7261 aaccgtcatg cgcggtgacg agattaatcg cggcactcgg cagacgacca ttacgtttaa
     7321 aaacatcgct ggaggcagca aaacgcccgg caaacgcccc cagaggcaaa tcataatgta
     7381 gccagaaacg acgggcagca tcgcggaaat gatcgttcca ctcggcaaac agcggcggga
     7441 aatttcccac ctgataacca ccaggagcga tatcccacgg ttcagcaatt aacttcacct
     7501 gcgagagcac cgggcagttc tggatagcgg taaacaacgg cgcatcctga cggaactctg
     7561 gcgtacggcc catgactgcc gccagatcaa agcggaaacc atcgacgtgg caggtttcta
     7621 cccaataacg caggcaggcg ctggcataat ccaccaccgc cggatgactc aaattgagcg
     7681 tgttgccgca accggtccag ttgtgataat cgccgtcttc tcttatccaa taatagctac
     7741 ggttatcgat cccacgcagc gagaataacg ggccgtcgag gtccagttcc gcactatggt
     7801 tgagcacgat atcaagaatg acttcgatac ccgctttatg cagtgctttg attgcatcgc
     7861 gaaactcatc cagcgccgtt tctggcgagc aggcatacgc cggatgcagc gcaaacatcg
     7921 ccaccgggtt gtaaccccag tagttactta gccccatgcg ttgcagacgt ggttcactgg
     7981 caaactgcgc cactggcagc agttccagcg cggtaatgcc caattgtttc aaatagttga
     8041 tcatcaccgg atgcccgagg gctttataag tgccacggat ctcgaccggg atctccgggt
     8101 gcaagtacgt taatcctttg acatgggctt cataaatgat ggtgctgccc cacggcgtgc
     8161 gcggcggggc atcatcttcc cagtcatagt gatcaaccac cactacgcat ttcggcgcaa
     8221 tggcggcgtt gtcgcgatag tcaggttcat tatgaccggc gtgcagcagc gggttatctt
     8281 taaactcccc gtcaatttgc cgcgcgcaag gatcaatcaa caacttcgcc gggttaaagc
     8341 gatgcccctc ggcgggttgc caggggccat gaacgcgata accataacgc aaacccgggc
     8401 gcgcatccgg cagataaccg tgccaaatgt cgccactgtg ccctggcaag tcatagcgat
     8461 gttcctggcc attggcgtca aagacacaca gttctacccg ctcggcatga gcggagaaaa
     8521 gtgtgaagtt gacgccctga ccgtcgtaat gcgcgccgag gggagcgggt ttgccaatgg
     8581 cgagttgtgt cattctgcct cccgaaccag ccagatagtg gccagcggtg gtagcgtcag
     8641 gcttagtgaa tgctgacgac cgtggctggc aatctcatcg ctgtgtaccg tgccgccatt
     8701 gcctgcatta ctgccgtgat agtgcatgga atcggtattg aggatttcac gccatttgcc
     8761 cggctggttt atgccgaagc gataatcatg acgcggtacc ggcgtaaagt tactggcaac
     8821 gatgatttcg ttaccctctt tatcgcgacg cacaaagatc agcaccgagc gttctttgtc
     8881 atccaccacc agccattcaa agccgtacgg gtcaaaatcc agttcatgca ttgctttatg
     8941 gtggcggtag gtgaggttca gatcgcgcac cagacgctgg acaccgtggt gccagttatc
     9001 gccgccttcc aacagatgcc agtcgaggct ggcgtcatgg ttccactcgc ggccctgggc
     9061 aaattcgtta cccatgaaca gtagtttctt gcccgggaat gcccacatcc agccatagta
     9121 ggcgcgcagg ttcgcgaatt tctgccatgc gtcgcccggc atgcggtcga gaatcgattt
     9181 tttaccgtgg accacttcat catgcgacaa cggcaggacg aagttttcag tgtagttgta
     9241 gagaatcccg aaggtcagtt tatcgtgatg atactgacga taaaccgggt cgagcttcat
     9301 gtagtccagg gtgtcatgca tccagccgag gttccacttg taccagaagc ccagaccgcc
     9361 catatcctgc ggacgagaaa cgccagggaa atcggtagac tcctcagcca ttgtcaccgc
     9421 accggaaacc tgctcaccaa gaatacggtt ggtattacgc aagaattcaa tcgcttcaag
     9481 attctcgcgc ccgccaaatt cgttcgggat ccactccccc tctttacggc tgtagtcgcg
     9541 ataaatcatt gacgccaccg catcgacgcg cagcgcatca ataccaaaac gttcaatcca
     9601 gtaaagcgcg ttaccgacga ggaagttact gacttcacgg cgaccatagt tgtagatcag
     9661 cgtgttccag tcctgatgat agccttcacg cggatcgctg tgttcataca agttcgtgcc
     9721 atcaaattcg gcaagcgcaa agtcatcagt cgggaagtgg cctggcaccc agtcgagaat
     9781 cacgttcaga ccagctgcgt gtgcggcatc aatgaaataa cggaagtcgt cgcgagtacc
     9841 aaaacggcgg gttggcgcat acaggccggt tggctgataa ccccaactgc catcgaaggg
     9901 atgctcgtta atgggcagta gttcgaggtg ggtaaagccc atccatttag cataaggcac
     9961 cagttgatcg gccagctcgc ggtagctcaa ccagaaattg ttgtcggtgt ggcgacgcca
    10021 ggaacccagg tgaacttcat aaatagagat tggcgcatca aactgattcg cttttttgcg
    10081 ctcttcagtc tgtacaacct tttccggcag cccgcaaata agagacgcgg tttccgggcg
    10141 catttgcgct tcaaaggcat aagggtcgga cttcagacgc aagttgccat tggcatcaat
    10201 catctcgtat ttatagagct gaccgttatg cgccccaggg ataaacagtt cccagatgcc
    10261 gctctcttta cgcaggcgca tcgggtgacg gcgaccgtcc cagtagttga attgcccaac
    10321 caccgagacc cgacgggcgt ttggagccca gacagagaaa cgcgtacctg tgacgccatc
    10381 catagtatct gcatgcgcgc ctaaggtttc atacgggcgc aggtgagtac cttcagataa
    10441 tagccaggca tccatttcct ggattagcgg accaaaacgg taaggatcat caatcaggtt
    10501 ttgctgacca tgccagacaa cagccaactg atagcggaaa aaattcttac gtcgcggaat
    10561 gacgccgcta aagaatcccc gtgagtcgag acactccagt tttgcgagtt tgcgcccggt
    10621 tttcggttca atcacccaca catcggtagc gtcgggtaaa agggcacgga cttccagtcc
    10681 cgcggtggtt ttatgcattc ccagtacgga aaaaggatcc gcaaaatggc ctgcaattag
    10741 cgcgttaatc acgtctctat cgatacgatc ggacatgctt gtcttcctgt tttattgtgt
    10801 caccccatcc aattggattt ttgacttctg gttgtgacat ttttttgacc tgaacggcgc
    10861 agcactctgt gcatcctctc tgcgtcgtcc tcacttcagg taaggctgtg aatactcatg
    10921 tattcagcca cccttaaaga atagccaatg ctctatttaa ctcccggtaa atcatgaaac
    10981 atctgcgctt actcctgtat tacgcactaa caggggcggc atcgcgcccc agatttaatg
    11041 aataaagatt acgccagttg acgaagcatc cgacgcagcg gctccgcggc cccccacagc
    11101 agctggtcgc ccactgtaaa ggctgacagg aactctggtc ccatattcag cttacgcagg
    11161 cggcctaccg gcgtggtcag cgtgccggta acggcagctg gggttagctc acgcatagtg
    11221 atttcccgat cgttcggaac gactttcgcc cacggattgt gcgcagccag cagttcttcc
    11281 acggtcggaa tagacacatc ttttttcaat ttaatagtga atgcctggct gtggcagcgc
    11341 aatgccccga cacgcacaca taaaccatct accggaatta cggaagatgt gttgaggatc
    11401 ttgttggttt ccgcctgccc tttccactct tcgcggctct gaccgttatc gagctgtttg
    11461 tcgatccacg gaatcaggct acccgccagc ggcacgccaa agttatccac cggcagctca
    11521 ccgctacggg ttaaggttgt gactttgcgt tcgatatcga gaatagcaga ggacggggtc
    11581 gcgagttcat ctgccacatg gccatacaga tggcccatct gggttaataa ctcacgcata
    11641 tgtcgcgcac caccgccgga agcggcctgg taggttgcaa cggacaccca atcaacaaga
    11701 tcattggcga ataaaccacc caacgacatc aacatcaggc ttacggtaca gttaccgcca
    11761 acaaaagtcc tgatgccatt atttaatccg tcggtaatga cgtcctgatt gacggggtca
    11821 agaatgatga tggcgtcatc tttcatgcgc agagacgatg ctgcgtcaat ccagtaacct
    11881 tgccatccgc tttcacgaag ctttggatag atttcgttgg tataatcgcc gccctgacag
    11941 gtcacaatga tatcgagggc ctttagcgcc tccagatcaa aggcatcctg aagtgtgcca
    12001 gtggttccgc caaaagacgg cgcagcctgg ccaagctgag aagtagaaaa gaagacaggg
    12061 cgaatggcgt cgaagtcgcg ctcttcaacc atgcgttgca tgagaacgga gccgaccata
    12121 ccgcgccagc cgataaaacc aacatttttc ataagcgttt tttcctgcaa agatgtgtgc
    12181 tgtataaatg tgccggtctc ctcttggcac atctttcacc atacaaaaag cagccaaagt
    12241 cgcaagtgaa attaatcaat gatagcgaag ccatcagtaa tgcgacttat cctgctttgt
    12301 tagcacgcag aaagtccgcg gcaattatca gggaatttga gttatgaatg aaatcatttc
    12361 tgcagcagtt ttattgatcc tgattatgga tccgctcgga aacctaccta ttttcatgtc
    12421 cgtactgaaa catactgaac cgaaaagacg gcgggcaatc atggtgcgag agttgcttat
    12481 tgctctcctg gtgatgctgg tgttcctgtt tgcgggtgag aaaattctgg catttctcag
    12541 cctgcgagca gaaaccgtct ccatttctgg cggcatcatt ctgtttctga tcgccattaa
    12601 gatgattttc cccagcgctt caggaaatag cagcgggctt cctgcaggtg aagagccatt
    12661 tatcgtgccg ttggcaattc cgttagtcgc cgggccgact attctcgcca cgctgatgtt
    12721 gttgtctcat cagtacccga atcagatggg gcatctggtg attgctctgc tgctggcctg
    12781 gggcggcacc tttgtcatcc tgctacagtc ttcgctattt ttacgtctgc tgggcgagaa
    12841 aggggtgaac gcacttgaac gcctgatggg attgattctg gtgatgatgg caacccagat
    12901 gttcctcgac ggcattcgaa tgtggatgaa ggggtaacga ggagagtttt tcgaaaagaa
    12961 cccggtagcg tcacaactac cgggcgaaca aacttaactc aacagctgga acgcaatcat
    13021 cccaacgatg gcaccgacag tgccgagaat ggtttccatc atggtccagg ttttcagcgt
    13081 ttcggcttcg gtcgcgccgg taaatttacc gaacaaccag aaaccagcgt cgttaacgtg
    13141 gctgacaaca atcgaaccac cagcgataca aatcgacagt gccgccattt gcgcaccaga
    13201 gtagttcagt tgttcaatga ctggcatcac cagtcccacc gccgttaaac aggctacggt
    13261 ggcagacccc tgaatgatgc gcactgcagc ggccagcacg aagcaggtga tagcaatcgg
    13321 caggcccatg ccggttaacg cttcgcccag tgccggacct acgccagagt caaccagcac
    13381 ctgtttgaac acgccgcccg caccaatcac cagcagaatg atccccgccg gttgcagcgc
    13441 gtgaccgcaa atctccatca ctttgtcttt tggcatgccc tgacgcatcg ccaggccata
    13501 aatcgccacc agacaagcaa ccagaatcgc ggtaaacgga tgaccaataa actcgaacca
    13561 ttcgtaagcg gtagagcctt ctggcacaaa acgcgcggca atggttttca gccccaccag
    13621 caccagcggc aacaggatca gcgacaggct gaatccgaag gacggcattt tgccttcgcc
    13681 gagatgcggt tcgctgatgt cgtcaggaat atgcaactcc acgtaacggc tgatgaaatt
    13741 accccacagc ggcccggcaa taatcattcc cggaattgcc gcacacaggc caatcaggat
    13801 catccagcca aaatcggcgt tcatctgcga tgccagcagc attggcgctg gccctggcac
    13861 caggaacgcc gccgctgccg ccacgcctgc gaataatggg attaccagct tcaccaggtt
    13921 cgtaccggtg tggcgcgcca ttgagaaagc aacgctaatc agcagaacaa tcgccacttc
    13981 aaagaacagc ggtagcgcac agaccagccc cgcaaggccg atggcataat gcgcgcggct
    14041 gtgaccgaag gatttgagca ttttgacggc aatctgatcg actgcgccgg tttcatgtaa
    14101 gatcttgcca aacatagctc ccagggcgac aaccaccgcc aggaagccga gggtgcctcc
    14161 catccctttt tccatcgtcg ctgcgatttt atcgagcggc ataccggaaa aaaggccagc
    14221 ccccatggac accaccatta aagccaggaa agcgtgcata cgcgccttca tgactaaaaa
    14281 cagcagcagt aaaacagacc ctactgctgt taaaacaagc gttaatgtag tcactactta
    14341 tttgcctttt ttaataacct caatggtgct tgccacaaca ccttccagcg gttgatcgat
    14401 atccaccacc agtacatcag tttcgtccgc acccggctcc tgcagcgttt caaactgcgt
    14461 caccaacatt tgggttttaa agaaatggcc tttgcgcgct ttcaggcggc tttcaatcac
    14521 gtcaaaatcg cctttcaggt agatgaaaga gagattcgga ttaccttcac gcagcaagtc
    14581 gcgatagtgt tttttcaatg cagaacagac gatcagtgac actttattag tgcgctgcat
    14641 agcaaacgcg gcgtcgttca gcgcctgcaa ccagggtttg cgatcgtcgt cattcagcgg
    14701 ttcgccagac gccatttttt cgatattgcg ccgtggatgg aggaaatcgc catcaagaaa
    14761 cgcggcatga agttgatgcg ccacttcact ggcgaccgca gatttgccgc tgcccgatac
    14821 gcccatcaag acgtaaatgt ggtgatcatg gttagtcgtg ctcaaagtgg tgcccccaca
    14881 atacaagaat taaaaatgtt acgggtaact gttatcggta acattgtcca gccggacaat
    14941 atcagaagca atatccatcc gtgccttaag tgtataagtg tgagctactt caaatttgta
    15001 ggcttaaata gatccgcccg gtgacaaggt gaaacctaaa tctaacattt tcggtgtcac
    15061 agattcgcca cgaatacgcg ccagcaggcg ttcagcgcca atactgccca tccgctcacg
    15121 cggcgtcagt acgctcgcca accgtggttc catcacctga ccaatgtcat gaccgtggaa
    15181 accggcaatc gccatatcgt caggaacttt taaccccaga cgctggcatt caaacgccgc
    15241 gccgaccgcc aggtcatcat tagtacagaa cacgccatcc agctgcggat attcccgcct
    15301 cgcctggcga atcagttcaa taccggaaga gtaagaagaa gattgctcaa ccatcacgct
    15361 atatggcacc aggtctgcat ccagcatcgc ctgttcgtat cccttctgtt tgatgatagt
    15421 acgttcgtcg agacgtgcgc cgagataagc aatgtggcga tgcccgcgag caataatggc
    15481 ggtggtcatc tggcgtgccg cttcaaagtt atcaaaaccg acggcaatat cgaggcacgg
    15541 cgacttgctg tccatcaact ccaccaccgg gatccccgcc acttcaatca tctttaaggt
    15601 gcgtggcgtg tgagtacgtt cggtgaggat cagtccatcg atattccatg agagcatgga
    15661 ttcgaggcgt tcttgctcca tttccggttt ataaccgtag tgcgccagca tggtttgata
    15721 accgtgcgcg tcggtgacgc tttcgattcc gcgtaatact tccgcgaaaa cctggttggt
    15781 gagagaaggt aacaggacgc caatcgcccg gctggtggcg ttagagagga tatcgggcgc
    15841 acgattggga atataaccca gttcatcaag agcggcggca atcttgccgc gtagagcgac
    15901 ggaaacctgc tccgggttac gcaaaaagcg gctgaccgtc attttggtca cgcctacacg
    15961 gtcagccaca tcctgaagta cgggtctttt ctttttcatc gtcctgaagg tacaaaagag
    16021 atagattctc ttagtttaac acggacgcat cagaaccttc ccggggaaaa cagtgcttgt
    16081 ttacaattat tagatataca ctaaatcaat tgcatcgcat tgtgctaacg acgcggcaaa
    16141 accccggaag cgtgcatcag cacgcgaccg gagcgagcga ggaagcctgc aaaaaggcag
    16201 gcttcaaaga tgccgttgag ttttaaaccg gcggcagatc gaacagtaac acttcgctgt
    16261 cgctatccgc atggatggag attgcctgct catcccagat tgccagacca tcgctggtcg
    16321 aggctttcac gccgttaatg gtgacattgc ctttcaccac ctggatccag acgcggcgtt
    16381 cagcggcaat ctgatgcacc gactgctcat ctttcagcaa cgcccaacga tacagttcca
    16441 tatcctgatg cactttcaat gaaccatctc gagcatccgg cgagagcacc agctgtttgc
    16501 cctgtacggc atcgaagcga cgctgttcat aacgcggcgt aatgccattt tcttcaggca
    16561 tgatccagat ctgatacaga tgcagacgct cggtgctgct tgggttgtac tctgagtgac
    16621 gaatacccgt acccgcactc ataatctgga actcacccgc cggaacctgc tctttattgc
    16681 ccatgctgtc ctgatgctca acagtacctt ccagcacgta ggtcaaaatt tccatatctt
    16741 tatgtgggtg agtgccgaag ccctgccctg cttcaatcac gtcgtcgtta atcacgcgca
    16801 gcgcggagaa gcccataaag ttcggatcgt aatagttggc aaaagagaaa gtatgccagg
    16861 agtccagcca gccatgattt gcatgaccac gttcatttgc tttgcgtaag tagatcattt
    16921 gtattcgccc cctgaatgat ttcgatgggc ttagtgtgga cccgatctgc ctgggatgat
    16981 agagggtgaa aattgacccc tctgttcaaa aaaattgaac tattcgaggg gccatcaaac
    17041 ttacttcgcg agagtcactg tggagggaga ggcttgctcg aatccgcgtt caaggatttc
    17101 cagattcgta agaacttcag attccttgac gtaatttggc gcaccgtggg tgatggtttg
    17161 atacaacgca tcataaacgc gcccgtaatc gcccatctcc ggcttaatct cttctctgac
    17221 cgtcacgccc tcgtcattga catactccag cacaccgacc gaatcatccg ctgagaatcc
    17281 cggttcgccc ggcataatat tagccttcag gctggtttcc tgctggtcga taccatattt
    17341 aataaacgaa cctttcttac cgtgaacgat aaatttcgga taatcgattt tcaccagatg
    17401 gctggttttg acgatggctt ttagatcgcc ataaaacagt tgcgcttcga aagtatcgtc
    17461 aggattggct ttattgcgca ggctgcggat gtcataagcg acgtgatccg ggcgaccgaa
    17521 cagagaaata atctggtcca tcgtatgcac accaaggcca tagaacgcgc catcctgcgg
    17581 caacccaggt ttggtttctg cgaccgggcg gtaatagtca aaatggcttt ccacttcaac
    17641 aatctctccc aacttgccac tttcaatcgc ttttttcgct gtcaggaagc aggagtcaaa
    17701 gcgacgattc tgatacggcg tgacggtcag ccctttgctt ttcgccaacg caaacagctc
    17761 tttcgcctgc gcaagtgtcg gagtgaacgg tttttcgacc agcacatttt tcccggcttc
    17821 cagcgcgcgt ttcgcgtact cgaaatggct gtccgcgtgg gtgcagacaa caaccagctt
    17881 aacatcggga tcgtttagta cttcgtcgag atcgctggtg aaatggatat gggaataaat
    17941 gggagcctgt tcttccggct tcgcatggcg acgaaaaata tgcgcgacat gccagctatc
    18001 cttgcggtta agtacatacg gcagatggta acgggtggtg cttttgccga agccaataaa
    18061 ggcgcagttg atgaccatga tttcgtcctt tttaaggtgg ttatttacac cttagcgcaa
    18121 agcggacgtg gttcctactg gagtgcgcat aacgaacaca agcactcccg tggataaatt
    18181 gagaacgaaa gatcaaaaaa aaagccagca cccggctggc taagtaatac tggaagcaat
    18241 gtgagcaatg tcgtgctttc aggttctccg cgagggtctt cctgattgcg agacaataat
    18301 aatcattctc attcgcactt gtccaacact ttttgcaaaa aaatgcattt gactcgcatt
    18361 tgaaagtcaa tgatgttgaa agggacattt accccaaaga ggacaaagga atgagtgaga
    18421 tagtaatacg ccatgctgaa acgcgcgatt acgaggccat caggcagatt cacgcccagc
    18481 cggaggtgta ttacaacacg ctacaagtgc ctcatccttc cgattatatg tggcaggagc
    18541 gactcgccga tcgtcccggc atcaagcaac tcgtcgcctg tattgatgga gacgtcgtgg
    18601 gccatctcac cattgacgtg caacagcgcc cacgccgcag tcatgttgcc gattttggta
    18661 tctgtgtcga ctcccattgg aagaatcgcg gcgtcgccag cgcccttatg cgagagatga
    18721 ttgaaatgtg cgacaactgg ttgcgggtag atcgcattga actaaccgtg tttgtcgata
    18781 acgccccggc aattaaggtc tataaaaaat acggctttga aattgaaggg actggtaaga
    18841 agtacgcatt gcgtaatggt gaatatgtcg atgcgtatta tatggcgcgg gtgaagtaag
    18901 atagtgccct ttttctgaga tgggaaaagg gtgtcattca aaatcggcat accttccttt
    18961 aatgtattta ttttcccaat acatatattg agatttacct cataattact tcataaagtg
    19021 taatatttta tttttta
//
LOCUS       DAZFZC010000092        18932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFZC010000092 DAZFZC010000000
VERSION     DAZFZC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.093"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..555
                     /locus_tag="ACHKXP_003797"
     CDS             <1..555
                     /locus_tag="ACHKXP_003797"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7648723.1"
                     /translation="KPRSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYEN
                     DARTLFEFTSGVNVTESPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRL
                     GGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYIASFDEM
                     VPEFIEKMDEALAEIGFVFGEQWR"
     gene            552..734
                     /locus_tag="ACHKXP_003798"
     CDS             552..734
                     /locus_tag="ACHKXP_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO7648724.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            707..898
                     /locus_tag="ACHKXP_003799"
     CDS             707..898
                     /locus_tag="ACHKXP_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7648725.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            975..1190
                     /locus_tag="ACHKXP_003800"
     CDS             975..1190
                     /locus_tag="ACHKXP_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7648726.1"
                     /translation="MTPQQENALRSIARLANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1289..1510
                     /locus_tag="ACHKXP_003801"
     CDS             1289..1510
                     /locus_tag="ACHKXP_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7648727.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            1552..1923
                     /locus_tag="ACHKXP_003802"
     CDS             1552..1923
                     /locus_tag="ACHKXP_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7648728.1"
                     /translation="METVATPQKLLAFRMKVTPQVVLALLDERERNQQYIKRRDQENE
                     DIALTVGKLRVELEAEKQRAKDLFMENARLKSGIAGLIHLGIRYADVEVMRIAGDAQL
                     STPCTDSIINSIATGIRIKGE"
     gene            1925..2050
                     /locus_tag="ACHKXP_003803"
                     /pseudo
     CDS             1925..2050
                     /locus_tag="ACHKXP_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            2048..2590
                     /locus_tag="ACHKXP_003804"
     CDS             2048..2590
                     /locus_tag="ACHKXP_003804"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO7648729.1"
                     /translation="MRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWAD
                     MQFLLWDAQRRAGISDAEITAAMENKLKINMERQWPEPKDGEPRLHIKEPGNSPVTPD
                     GWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGKPTWDDGDGED
                     LYLKEVTHWMQLPEPPQEVK"
     gene            2678..2920
                     /locus_tag="ACHKXP_003805"
     CDS             2678..2920
                     /locus_tag="ACHKXP_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7648730.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            2924..3058
                     /locus_tag="ACHKXP_003806"
     CDS             2924..3058
                     /locus_tag="ACHKXP_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648731.1"
                     /translation="MHTAFEFWVRKTFGNRYGLTRDVDGFYCREVVKRMFDVWFHCRG
                     "
     gene            3077..3331
                     /gene="xisR"
                     /locus_tag="ACHKXP_003807"
     CDS             3077..3331
                     /gene="xisR"
                     /locus_tag="ACHKXP_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7648732.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            3365..4651
                     /locus_tag="ACHKXP_003808"
     CDS             3365..4651
                     /locus_tag="ACHKXP_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7648733.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            4672..5373
                     /gene="mlrA"
                     /locus_tag="ACHKXP_003809"
     CDS             4672..5373
                     /gene="mlrA"
                     /locus_tag="ACHKXP_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO7648734.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            5433..5540
                     /gene="yohO"
                     /locus_tag="ACHKXP_003810"
     CDS             5433..5540
                     /gene="yohO"
                     /locus_tag="ACHKXP_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO7648735.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(5521..6252)
                     /gene="yehW"
                     /locus_tag="ACHKXP_003811"
     CDS             complement(5521..6252)
                     /gene="yehW"
                     /locus_tag="ACHKXP_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO7648736.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(6257..7183)
                     /gene="yehX"
                     /locus_tag="ACHKXP_003812"
     CDS             complement(6257..7183)
                     /gene="yehX"
                     /locus_tag="ACHKXP_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO7648737.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNMQGQPCGTLHFQDLLVEA"
     gene            complement(7176..8333)
                     /gene="yehY"
                     /locus_tag="ACHKXP_003813"
     CDS             complement(7176..8333)
                     /gene="yehY"
                     /locus_tag="ACHKXP_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO7648738.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGARRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(8340..9257)
                     /gene="osmF"
                     /locus_tag="ACHKXP_003814"
     CDS             complement(8340..9257)
                     /gene="osmF"
                     /locus_tag="ACHKXP_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO7648739.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLREYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(9468..11765)
                     /gene="bglX"
                     /locus_tag="ACHKXP_003815"
     CDS             complement(9468..11765)
                     /gene="bglX"
                     /locus_tag="ACHKXP_003815"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO7648740.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11961..13676
                     /gene="dld"
                     /locus_tag="ACHKXP_003816"
     CDS             11961..13676
                     /gene="dld"
                     /locus_tag="ACHKXP_003816"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7648741.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHMLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(13714..14646)
                     /gene="pbpG"
                     /locus_tag="ACHKXP_003817"
     CDS             complement(13714..14646)
                     /gene="pbpG"
                     /locus_tag="ACHKXP_003817"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO7648742.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14820..15407)
                     /gene="yohC"
                     /locus_tag="ACHKXP_003818"
     CDS             complement(14820..15407)
                     /gene="yohC"
                     /locus_tag="ACHKXP_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO7648743.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            15577..16155
                     /gene="yohD"
                     /locus_tag="ACHKXP_003819"
     CDS             15577..16155
                     /gene="yohD"
                     /locus_tag="ACHKXP_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7648744.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHTLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(16285..17046)
                     /gene="yohF"
                     /locus_tag="ACHKXP_003820"
     CDS             complement(16285..17046)
                     /gene="yohF"
                     /locus_tag="ACHKXP_003820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7648745.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(17099..18535)
                     /gene="mdtQ"
                     /locus_tag="ACHKXP_003821"
     CDS             complement(17099..18535)
                     /gene="mdtQ"
                     /locus_tag="ACHKXP_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO7648746.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            18759..18842
                     /gene="yohP"
                     /locus_tag="ACHKXP_003822"
     CDS             18759..18842
                     /gene="yohP"
                     /locus_tag="ACHKXP_003822"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO7648747.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4758 a         5018 c         4784 g         4372 t
ORIGIN      
        1 aagccccgct caggaaagaa gtggcctgac atgaaaatgt cctacttcca caccctgctg
       61 gctgaggttt gcaccggtgt ggctccggaa gttaatgcta aggcgctggc atggggaaaa
      121 cagtacgaga acgacgccag aaccctgttt gaattcactt ccggcgtgaa tgttactgaa
      181 tccccgatca tctatcgcga cgaaagtatg cgcaccgcct gctctcccga tggtttatgc
      241 agtgatggca acggccttga actgaaatgc ccgtttacct cccgggattt catgaagttc
      301 cggctcggtg gtttcgaggc cataaagtcg gcttacatgg cccaggtgca gtacagcatg
      361 tgggtgacgc gaaaagatgc ctggtacttt gccaactatg acccgcgtat gaagcgtgaa
      421 ggcctgcatt atgtcgtggt tgagcgggat gaaaagtaca tagcgagttt tgacgagatg
      481 gtgccggagt tcatcgaaaa aatggacgag gcactggctg aaattggttt tgtatttggg
      541 gagcaatggc gatgacgcat cctcacgata atatccgggt aggcgcgatt actttcgtct
      601 actccgttac aaagcgaggc tgggtatttc ccggcctttc tgttatccga aatccactga
      661 aagcacagcg gctggctgag gagataaata ataaacgagg ggctgtatgc acaaagcatc
      721 tcctgttgag ttaagaacga gtatcgagat ggcacatagc ctcgctcaaa ttggagtcag
      781 gtttgtgcca ataccagtag aaacagacga agaatttcat acgttagccg catccctttc
      841 acaaaagctg gaaatgatgg tggcgaaagc agaagcagat gagagagacc aggtatgaca
      901 accacggaat gcatttttct ggcagcgggc ttcatatttt gtgtgcttat gcttgccgac
      961 atgggacttg ttcaatgaca cctcagcaag aaaacgccct tcgcagcatt gcccgtctgg
     1021 ctaactctga aatcaaaaaa gccagacagc agtttccgga taaaaacgtc gatgacattt
     1081 gccgtagcgt actgaagaag caccgcgaaa cggtaacgct gatgggattc acaccgactc
     1141 atttaagtct ggcgatcggc atgttaaacg gcgtctttaa ggagcgatga acatgaaaag
     1201 caaaatcatc agggagctac aggctccttt tttattgttc gcattcaccc tcaagcgtat
     1261 taaccaacaa ttcagggatt aatggaagat ggcagacatc attgattcag catcagaaat
     1321 tgaagaatta cagcgcaaca cagcaataaa aatgcgccgc ctgaaccacc aggctatatc
     1381 tgccactcat tgttgtgagt gtggcgatcc catagatgaa cgaagacgcc tggtcgttca
     1441 gggttgtcgg acttgtgcaa gttgccagga ggatctggag cttatcagta aacagagagg
     1501 ttcgaagtga gcgaaattaa ctctcaggca ctgcgtgagg cggcagtagc aattgaaaca
     1561 gtagcaacgc ctcaaaaatt gctggcattt cgtatgaaag tcacacctca ggttgtgctg
     1621 gcactgctgg atgaacggga aagaaaccag caatacatca aacgccgcga ccaagagaac
     1681 gaggatattg cgttaacggt agggaagttg cgcgttgagc ttgaggcaga aaaacagcgg
     1741 gcaaaggatc tgtttatgga aaatgctcgg cttaagtcag gtatagccgg tctgatacac
     1801 ctcggtattc gatatgcaga tgttgaggtc atgagaattg ctggagatgc ccagctttct
     1861 accccatgca ctgacagcat cataaacagc attgcaacag gcattcgcat caaaggagag
     1921 tgatatgagc gctataacca aagaacgtat caaattattc attaaaaatc cgcttgataa
     1981 cggacttact cgtggcgaac aaatggaact ggcacgaatt gcactggcat cactggaacg
     2041 cgaacagatt cgccacgagc atgccaaatg gtctgactcc acatttggct gcgttggccc
     2101 cattggtccg ctgaaacatc tctcaaaaga ggcactggaa gccgcagccg aacctgacga
     2161 tctcagcgag tgggctgata tgcagttcct gttgtgggat gcacagcgcc gtgctggtat
     2221 cagtgatgct gaaattaccg ctgctatgga aaataaattg aagatcaaca tggagcgcca
     2281 gtggcctgaa ccaaaagatg gtgagcctcg tttgcacatt aaagaacccg gcaactctcc
     2341 ggtaactccg gatggttgga taagctgtag tgagcgaatg cctgaaatgg gagagcgaca
     2401 atgctatgtg ttagcagctg actttaaaaa caactaccca ccaaacatcc ccaacactca
     2461 ggtcggcgta tatggcgact ggtttaatga tggcaagccc acttgggatg acggtgatgg
     2521 cgaagacctg tatctcaaag aggtaaccca ctggatgcag ctaccagaac cgccgcagga
     2581 ggtgaaatga tgaattggcc tgaagcattc accgcagttg gagttgcaat agcggtggca
     2641 tttattctgt attcgctttt ccgctgggga taaaggaatg ttcgctctga ttcaacgtgg
     2701 tcagatatac acggacagag ccggataccc tgtggtgatt actcgcagta ctcagcactc
     2761 agtgttcttt cgacgcatgg acgggcgctc cggacgggta cgcattggtg agttcaacaa
     2821 cctgttcgaa catattgacc aacaggagta ccgcaaaatt ctggcgggca ctgagcagga
     2881 aatgcgcctg aaaaaattac gcgcaatgca acggaggtga tacatgcata cggcttttga
     2941 gttctgggtt cgcaagacat tcggcaatcg ctacggcctg acccgtgatg tcgacggctt
     3001 ctactgccgt gaagttgtga aacgaatgtt tgacgtgtgg ttccactgcc gtggatgaaa
     3061 gttttatgag gttggcatgc agacaatcat ctatcagata acccccagca aatggtgtac
     3121 ggagagagtc cttattgcat caacagggct aaagcccggc accatcgagc gggccagaag
     3181 aaagtcatgg atgcagggaa aagaataccg ccattacgct gtagaaggtg atcctgggca
     3241 ttacagtgaa tgcctgtaca acatcgaaga aattatgcga tggatcgaaa accagaaaca
     3301 accaggtgcc aaaaatgcaa gttccggtta acctgttaat gctcctggac gtctgggagg
     3361 tttaatgagt aacgcatcat acccgacagg cgttgaaaac catggaggat cactccgtat
     3421 atggtttcac tataatggca aacgtgtcag agaaaacctc ggtgttcctg acacagccaa
     3481 aaaccggaag atcgctggtg aacttcgcac ttccgtttgt tttgcaatca gaatggggag
     3541 tttcgactac gccgcgcagt tccctaattc ccctaacctg aaacactttg gtctgggaaa
     3601 aagagagata accgttaagg cactttcgga aaaatggttg gaccttaaga aaattgagat
     3661 ttgtgcgaat gcacttaacc gttaccagtc agtaattaaa aacatgttac caatgttagg
     3721 tgaaaaaaaa ctggtttcat ccataacaaa agaggattta cttttcgtaa ggagagattt
     3781 gttgaccggt taccaaaagc tttctaatgg aaagacttct tccataaaag ggcgctcagt
     3841 ggtcacggta aactactata tgacaaccat agctggaatg tttcaatttg caacagataa
     3901 tggttatacc tcaggaaacc catttaacgg tctggctccc ttaaaaaagt ccaaggtaaa
     3961 accagatcct ctcacccgtg acgaatttat tcgttttatt gaggcttgcc gtcatcaaca
     4021 aacaaaaaac ctgtggattc tcgctgtata cacgggtatt cgtcacgggg agttggtatc
     4081 gctggcatgg gaagatatag accttaaagc aaggactata accatccgta gaaattatac
     4141 aaaacttggc gaattcactc caccaaaaac cgatgcaggc accggaagga caattcatct
     4201 ggttcaacca gctattgatg ctcttaaaag ccaggcggaa atgaccatgc ttggaaagca
     4261 acattctgta gaggtgaagc agagggaata tgggagaact gctgtgcata aatgcacttt
     4321 tgtttttagt cctcaggtaa caaaacagca gcagttgtcc ggacctcact acaaggttga
     4381 ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac acagaaaatc
     4441 gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gcaggagcta atcctagttt
     4501 tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt acggagcatg
     4561 gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat ctgaaagtgt
     4621 cccatgtatg ccccataaga aagcggggta aaataaaaac ttgcaaaacc aattggttta
     4681 cctttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct gaaaccgcaa
     4741 cggacagacg gcggtcatcg gctgttcaac gatgccgata ttgaccggat ccgcgagatc
     4801 aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct cagtaatgaa
     4861 aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac ttacctgcaa
     4921 agcggcaatc tacatagcct gcgaacgtgg atcaaagagc gcggtcagga ttaccccgcc
     4981 cagacactca ccacacatct gtttattcct ctgcgccgac ggcttcagtg ccaacaaccg
     5041 actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat cgccatttgt
     5101 ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg gaatattcag
     5161 gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg gcgcattgat
     5221 gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg tacattgctg
     5281 gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag ttggcaagaa
     5341 caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg cgctttactg
     5401 tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt ggggtcatcg
     5461 ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca ggttatacct
     5521 ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt ctgcgatgat
     5581 cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa atccgctaag
     5641 cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga tcgtcgccgt
     5701 accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag ccgccagcgg
     5761 tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg caacttctgt
     5821 cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg gcagcacacc
     5881 gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg gaacggcgat
     5941 cgccagcact gcgacgggcg gaaaagtctg tccaacggcg gcaatagttt ccaccagtgg
     6001 gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc caatgatcac
     6061 cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag ctgcaaaact
     6121 ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca gcggctggct
     6181 gtaaggcagc cagaaaatca gcgccacaaa cagagcaatg agccagaaca gcggatcgcg
     6241 caacatcttc atacgcttac gcctccacca gcagatcctg aaaatgcagc gtgccgcaag
     6301 gctggccctg catgttcacc accggcagca cctcgcatcc ccgcgcaaca aacagagaga
     6361 gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc atctgctcgt tcttcgcgac
     6421 gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttca ctacgtccaa
     6481 aaaactggcg gacaaaatca ttcgccggac gagtcagcat cgtcagcgga ttgccctgct
     6541 gcactacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc gcctcatcaa
     6601 tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc gtcatctctt
     6661 gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc attagtaaga
     6721 cttgcggatc ggcagccagt gcacgcgcca ctcccacacg ttgctgctga ccaccggaaa
     6781 gctgatgcgg ataacgctca cgcaaatttg actccagccc cagtagcgcc attaattcgt
     6841 cgatacgatc gtcaatccgc gcccgcgacc atttttgtaa ttgcggcacg gtagcgatgt
     6901 tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag cccatccggc
     6961 ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg atcactccgc
     7021 tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg gagccagatg
     7081 tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg ttaacggctt
     7141 tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt caccttcagc
     7201 agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat taccgggatc
     7261 acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac cagcgcacca
     7321 aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg caccatcacc
     7381 acccgcaggc tgcgcagaaa taccggtaac gccagtggta actgaacatg caggaatcgc
     7441 cgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg gatctggttc
     7501 aagccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag cgcaatcagt
     7561 gcgggtgtca ttccggttcc tgctatgccg agcttcccca gccacggaaa ggccgtcacc
     7621 agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac ggtctgaatg
     7681 acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca ccagatgccc
     7741 aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt cagatgttgt
     7801 gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag tgagagatcg
     7861 ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat atgcaacaac
     7921 cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc cagcaatgcc
     7981 agcgccgcag ccagccagaa accactgccg aggctggtac gcgccagcgc actgccattt
     8041 tgcgccagtt gggtcgccgc ctttccagct ccccacacca gcaatacgaa gacgaattgc
     8101 gccagaatga gtgcacaaat gctgcctttt ttaccgggaa taaaacaggc cgtcagccag
     8161 gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagctgcca gagatgacgc
     8221 ccctcacccg aaactaaacg attaggcgcg taactgataa acggcagcgc cgctgcgatt
     8281 gccgtcagca acagcagcag cgccagaaca ggattaatac ggaaataagt cacgggaaat
     8341 tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc atccagtcct
     8401 tccacagcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct ggcgaagact
     8461 ggctgtagcc actgtgccat ttgcggatac tccctcaaca ccgactcacg caccactggt
     8521 gcaggcgcgt agataggttg cacaccttgc ggatcgctta aggtttgcag ccccagcgcc
     8581 gctaccgggc cgtcagtgcc gtaagccatt gcagcattaa cgccagaggt ttgctgggca
     8641 gcggctttga tcgtcaccgc cgtgtcgcca ccagccagtg acagcaactg atcctgaccg
     8701 agcttaaagc cataggcttt ttcaaacgcg ggtaacgcat cggcgcgttc gataaactct
     8761 gccgaggctg ccagtttgaa ggtgccgccc tcttgcagat aacgactcag gtcggcaagc
     8821 gaagtgagtt tatttttctc tgccacatcc tgacgcacgg cgatagtcca ggtgttattt
     8881 gcaggcgcgg gcgtcagcca gattaacttg ttgtgctccg aatcgagctt tttgactttc
     8941 tcgtaacctt gctgcgcgtt tttccacgct gcatcgtttt catctttaaa gaagaaagcg
     9001 ccattgccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc cccgcgcacc
     9061 acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct ttccagcacc
     9121 tgcaaaatga tattgccgag tagcgcacct tcggtatcga tttttgaacc gactttaacg
     9181 ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga acctgcccag
     9241 agctttaaga gtggcatgat gctttcctca ttcttttact gttgttttca gcgaattaag
     9301 agaaaagcat agttgataat ggcggggtta gcttgagcgg aaggattcag ttgcagaatc
     9361 agataaatac ttaagaggca tattcggtcc ggcgtttcct gccggatgcg gcgcgagcgc
     9421 cttatccggc ctacaaaggg cgcaaacgtc gcgcccttac taaagcatta cagcaactca
     9481 aactcgcctt tcttaacgcg tgcggaatca gtgccgataa agacattgaa cttgccaggc
     9541 tcggcgtcat atttcatctg ttgattccag aacttcagcg cctcaatatc gatcgggaag
     9601 ctgacagtct gagtttcgcc cggtttcagg gtgattttct caaagccttt cagctgtttc
     9661 acagggcgac tcatggaagc cgtcacatcc tgcaagtaca tctgcactac cgtggccccc
     9721 tcgcgcttac cggtgttcgt cacctgcacg ctggcagtca ctttgccgtc acgcttcatg
     9781 gtcggcgcag aaagtttcac atcagatacg gtgaaagtgg tgtagctcag cccatagccg
     9841 aacggataca acgccccgtt agcttcatca aaataacgcg aagtgtattt gttcggcttg
     9901 tcggcattat acgggcgacc ggtattcaga tggctgtagt acaccgggat ctgcccgaca
     9961 gaacgcggga aggacattgg cagcttgccg gacgggttgt aatcgccaaa caatacatcg
    10021 gcaattgcat taccgccttc agtccccgca aaccaggttt ccagaatcgc atcagcctgc
    10081 tgatcttctt tcaccagcgc cagcggacgc ccgttcatca gcaccagcac cagcggttta
    10141 ccggtggctt tcagcgccgc aatcaagtca cgttggcttt gcggaatagt gatatcggtc
    10201 cggctggagg cttcgtgcgc catcccctgt gcttcaccga ctacagccac caccacatca
    10261 gattgtttcg ccgtctgcac cgcttcatca atcatctctt gcggcgaacg cggatcgact
    10321 ttgaccgctt cttcatactg attcaggaaa tcgataatgc ctttgtcact ggtaacgttc
    10381 gcccctttgg catacagcac tttaccgttt tcaccgacgg catttttaat cccggtcagt
    10441 acggtcacgg attgatcggc aacaccggct gcggaccagc tgcccatcac gtcacgttta
    10501 ctgtccgcca gtggcccaac caccgcaatg gtggccgatt ttttcagcgg taacgtttcg
    10561 agacggtttt tcagcaacac caggctttcg cgcgccactt cacgcgcttc tttacggtgc
    10621 aggcggcttt cggcattggt atccaccggg tcagactctt tcggccccaa atggctgtat
    10681 gggtcgttaa acaatcccat atcatattta acgttcagta catggcgggc agcatcgtcc
    10741 agctcttcca tcgtcacttt gccggatttg atcaacccag gcagatactt cgagtaatac
    10801 tcgtcgctca tactcatgtt gattccggat ttcagcgcca cgcgcaccgc atcttccggg
    10861 tctgccgccg tgccatgttt aatcagctct ttgattgcac cgtgatcgga aacggtgatg
    10921 cctttaaagc cccactggtc gcgcagaaca tctttcagca gccaggaatc ggaggtggct
    10981 ggcgtgccgt tcagcgagtt cagcgccacc atcaccgcgc cgcttcctgc gtccagcccc
    11041 gctttgtacg gcggcatata atcattaaac aggcgctgcg gactcatatc gacggtgttg
    11101 tactctttac cgccttctac cgcgccgtat gcggcaaagt gtttgacgct ggtcatcacc
    11161 gagtagcgat ctgccgggct tttaccctgc atcgcttcca ccatggtttt acccattgtt
    11221 gaggtgagat acgtatcttc gccaaaacct tcggaagcac gtccccagcg cggatcgcgc
    11281 gagacatcga ccatcggtgc ccaggtcata ttcaggccat catctgccgc ttcataagca
    11341 gagacacgtc cgaccgtttt cactgcatcg aggttaaaag acgaggccag accgaggcta
    11401 atcgggaaca ccgtgcgctg accgtgcagc acgtcgtaag caaagaaaag aggaattttc
    11461 aggcggctta attccatcac ctgatcctgc atggcgcgga tatcctgacg ggttacggtg
    11521 ttgaaaatcg ccccaacctg accgtctttg atcatctcgc ggatcgcctc tttcgggtta
    11581 tccgggccga cgctgattaa gcgcagctga ccaattttct catcaactgt cattttctta
    11641 agcagttcgg tgacgaacgc atcccgcgct tcgggcgtta atggatggtt gccgaataaa
    11701 tcatccgcca gtgctggctg cagggccaga ctcaccgcga ttcctactga acatagccat
    11761 ttcatatgga tttttcttcc tctgttgccc gacgaggcag cgaaaatggt gcaaaaaccg
    11821 tagtttgcca taagcatgat ggagagagaa aaagaatgct cagtttattg tctgaatttt
    11881 caaaatattc actcgctgaa ttgtcataca aggcgctatt ctagtttgtg atattttttc
    11941 gccaccacaa ggagtggaaa atgtcttcca tgacaacaac tgataataaa gcctttttga
    12001 atgaacttgc ccgtctggtc ggtcattcac acctgctcac cgatcccgca aaaacggccc
    12061 gctatcgcaa gggcttccgt tctggtcagg gcgacgcgct ggctgtcgtt ttccctggct
    12121 cactactgga attgtggcgg gtgctgaaag cctgtgtcac cgccgacaaa attattctga
    12181 tgcaggctgc caatacaggc ctgaccgaag gatcgacgcc aaacggtaac gactatgatc
    12241 gcgatatcgt gatcatcagc accctgcgtc tcgacaagct gcacgttctt ggcaagggcg
    12301 aacaggtgct ggcctatccg ggcaccacgc tctattcgct ggaaaaagcc ctcaaaccgc
    12361 tgggacgcga accgcactcg gtgattggat cgtcgtgtat aggcgcatcg gttatcggcg
    12421 gtatttgtaa caactccggc ggctcgctgg tgcaacgtgg cccggcgtat accgaaatgt
    12481 cgttattcgc gcgtataaat gaagacggca aactgacgct ggtgaaccat ctggggattg
    12541 atctgggcga aacgccggag cagatcctta gcaagctgga tgatgatcgc atcaaagatg
    12601 acgatgtgcg tcacgatggt cgtcacgccc acgattatga ctatgtccac cgcgttcgtg
    12661 atattgaagc cgacacgccc gcacgttata acgccgatcc ggatcggtta tttgaatctt
    12721 ctggttgcgc cgggaagctg gccgtctttg cggtacgtct agataccttc gaagcggaaa
    12781 aaaatcagca ggtgttttat atcggcacca accagccgga agtgctgacc gaaatccgcc
    12841 gtcatattct ggctaacttc gaaaatctgc cggttgccgg ggaatatatg caccgggata
    12901 tctacgatat tgcggaaaaa tacggcaaag acaccttcct gatgattgat aagttaggca
    12961 ccgacaagat gccgttcttc tttaatctca agggacgcac cgatgcgatg ctggagaaag
    13021 tgaaattctt ccgtccgcat tttactgacc gtgcgatgca aaaattcggt cacctgttcc
    13081 ccagccattt accgccgcgc atgaaaaact ggcgcgataa atacgagcat catctgctgt
    13141 taaaaatggc gggcgatggc gtgggcgaag ccaaatcgtg gttggtggat tatttcaaac
    13201 aggccgaagg cgatttcttt gtctgtacgc cggaggaagg cagcaaagcg tttttacacc
    13261 gtttcgccgc tgcgggcgca gcaattcgtt atcaggcggt gcattccgat gaagtcgaag
    13321 acattctggc gttggatatc gctctgcggc gtaacgacac cgagtggtat gagcatttac
    13381 cgccggagat cgacagccag ctggtgcaca tgctctatta cggccatttt atgtgctatg
    13441 tcttccatca ggattacata gtgaaaaaag gcgtggatgt gcatgcgtta aaagaacaga
    13501 tgctggaact gctacagcag cgcggcgcgc aataccctgc cgagcataac gtcggtcatt
    13561 tgtataaagc accggagacg ttgcagaagt tctatcgcga gaacgatccg accaacagca
    13621 tgaatccggg gatcggtaaa accagtaaac ggaaaaactg gcaggaagtg gagtaaaaat
    13681 tacggatggc agagtatcgc catccgaatt cacttaatcg ttctgtgccg tctgccccgc
    13741 cgccgccatt tgggcggctt tttgtttttt atagctcaac gctgctgccg gcacaggcat
    13801 cactttaccg gtttcaatcc aggtacgcag gcggctggca tcggcaaaat gggtatattt
    13861 gccaaacgcg tccatcacta ccagcgccac cggtttatta ttgataaccg tacgcatcac
    13921 cagacaatgg cccgccgcat tggtaaagcc ggttttggtt aactgaatat tccagttatc
    13981 gcgatacacc agatgattag tattgcggaa cggcagcgta tacgtcggat tagagaaggt
    14041 tgccatatct tcccgggtag tacttaactg cccgatcaac ggatattgtt tgctggcaat
    14101 gagcagtttg gttaagtcac gggcagttga aacgttatgc accgacaatc cggtaggttc
    14161 aacaaagcgc gtgttgttca ttccgagcga tttcgctttc gcattcattg ccttaataaa
    14221 ggctttgtaa ccaccgggat aatggtgcgc aaggcttgcc gccgcgcggt tttctgaaga
    14281 catcagcgcc agcaacagca tatctttacg gctgatttcg ctattcagtc gtacgcgcga
    14341 atagacccct ttcatctccg gcgtctggct gatatccact tttagttttt catccagcgg
    14401 cagtcgtgca tccagcacaa ccatcgcggt cattaatttg ctgatagacg caatcggacg
    14461 caccagatcc gggtggttcg aatagatcac tttgttggta ttcagatcaa caatcatcgc
    14521 gctaccggag gcaatttccg gttgtgaagc ggtggtagcg gctgccgttt tcgcaacggc
    14581 ctgcggtgcc aaaggcacag ccagcatcag ggccaggcta aataaagaaa ctcgaaattt
    14641 cggcatgatg agcattcaga tagtggttca cgcgcacggg ttgcgcaccg ccggagtaag
    14701 gatttactga ggctagcgac gccatcataa cgagcaaaaa gtgcgatcgt caaaggagaa
    14761 tcgtgaggaa atgctgcatt gctgacattt acgccagcaa tgcaacgtca aagaactttc
    14821 tagaacaaac gataaccgta gccccatagt ataacggtta gggcgagcag cacttccagt
    14881 accagcacgc caatcgccag cgtcgaacta gagaagctaa ggccttcctc tttgttgata
    14941 ttcaggaagc tcggaatacc aaggtaaagc aggtagccgg tgtaaaacag cgccaccgtg
    15001 ccgaccagcg cacacaacca gaccagtgga taaagcgcca ccagaccgct taaaaacagc
    15061 ggagttgcaa cgtagcccgc gaagaccatg cagtgcgcaa gtgacggacg ctgcggataa
    15121 ttacgcgcca tccaccagat gacccgcccc attaccgcaa ccccagccag cataacgcca
    15181 taaaatagga cagccagcgc cagtccggta aaccaggata acttcaggat agtgccatcg
    15241 ccaaaattcc agccaatctg tgtagtgcca atgaaggcgc aaatcaccgg aatcgccgcc
    15301 atcagcaaaa cgtggtgggt gtaatggtga gaaatcgttt cgttttcgcg attaatcacc
    15361 tgcatttcac gatcgggatg ggaaaacagt ccccagacat ggctcatacc gcccccttgt
    15421 tgtgagttca tgaacctgac agttcaagta taagtcagct tgtgattatt ttttgttcgc
    15481 tcatgtaatt tgcaccgtct gatgtacgtc ctttttcagc gtgaatgatt tccagggtgt
    15541 atgcttaaag gcaaccaaag ggagacagac tggcctatgg atctcaatac actcatctca
    15601 caatatggtt atgccgcgct ggtgatcggt agcctggcgg aaggtgaaac cgtgactttg
    15661 ctgggaggcg ttgcggcaca tcaggggcta ttaaagttcc cgctggtggt actttctgtg
    15721 gcgcttggcg gcatgattgg cgaccaggtg ctttatctgt gcgggcggcg gtttggcggc
    15781 aagctgttac gccgtttctc gaaacatcag gataaaattg agcgggcgca gaaacttatc
    15841 caacgccatc cgtatctgtt tgtcattggt acgcgcttta tgtatggctt tcgggtgatt
    15901 ggcccgacgc tgattggtgc cagccagctg ccgccgaaaa tctttctgcc gcttaatatt
    15961 ctcggcgcat ttgcctgggc gttgattttt accactatcg gttacgctgg tggtcaggtg
    16021 attgcgccgt ggttgcacac tctcgaccag catttgaagc actgggtctg gttgattctg
    16081 gtcgtggttc tggtggtagg cgtgcgctgg tggctgaaac ggcgcgggaa gaaaaagccg
    16141 gatcatcagg cgtaaaacca ttgccctgga taaggcgttc gcgccgcatc cgacatccgg
    16201 agtaccatgc ctgatacgac gcttgtgcgt cttttcatgc cgaccaaaat attacccaat
    16261 gaaatatcca cgcacctcac tgcgctattc tgggttgaac tgtggattcg ccaacataaa
    16321 gccgccatcc actatcaacg actgcccggt ggtgtaattt gcgccctccg aacaaagcca
    16381 caccaccagg ctggcaatct catgcgttgc gccaaaacgc cgcaagggaa tcgaaggctc
    16441 cgcgtcgggc ttcacgtcgc tgtcatccat gccattcatt ggcgtggcga tcgccccagg
    16501 cgcgactgcg ttcaccaaaa tcttatgcct gaccagctcc agcgccatcg ctttggttaa
    16561 cccaccgagc gcatgtttag cggctgtgta ggcgctggca tccggcagcg gcgtatgttc
    16621 atgtaccgac gtaatgttga tgatgcgacc gccctgccct tgtttcacca tctgacgagc
    16681 cgcaatttgc gagcataaga atgcaccatc gacatcaacg gtaaaaatct tgcgccactc
    16741 atcaaaagcc atatcaagaa acggcgcttt ggtcattgca cccgcattat tcaccagcac
    16801 atcaatgcgc cccagccgtt gaatgagttt ctccagcgcc agtgcccctt ctggtagatt
    16861 gccgagatcc agctgcacga tctccgcacg tacgccgtgg ctaactacct cacgcgcggt
    16921 atcttttgcc ccttcttcat ctgagtgcca ggtaatacca atatcaaacc cctgctgcgc
    16981 cagtaataac gcgcactctt tgccgatccc cgaatcggag gcggtaataa tcgcaacctg
    17041 tgccatcgag ttctccactt aacgctgaat aaacgttaag tatagaaggc gcatatcatc
    17101 agcgtttgta acccccgccc aacgcaccag tgagttgaat ggaggcatcc agccactgcc
    17161 cttgcaataa caggccattg gcccgctcac gcagcgcggg gattctggct tcgctgacgc
    17221 gggaaccagc aatgatgccc gcgttaaagc gcgcctgcgc aagacctacc acacgcaagg
    17281 catcgcgctc aatttgcgcc tgatgctggt ttttctccgc cagtgtctga acctgactgg
    17341 ctgcccgcgc cacgtcattc accgcttcaa ccaccgcttt gttgtagctg gcgatagaca
    17401 agttgctttc ggcttttgcg atatcgagat tggcgttaag acgaccacta tcgaaaatgg
    17461 gtagcgtcag gcctgccgta acgcccattt gctgcgcgga atgacggaac agatcgctta
    17521 agtgcaacgc atcctgttgc aggaaggcca tcaggttgat gtcaggataa aatgccgctt
    17581 ttgccgcatc aatggtgctt agcgatgact caacgtacca gtgcgccgcc tgcaaatctg
    17641 cccgccgggc cagtaaggag taccccagtt catcaggaag ctggcttgcc actttcggca
    17701 acgcgaccgg tttaagcttc aatgactttg tctggttatt tgtaagtgcg cttaaccgtg
    17761 cctcaataat tttcattttc cccgcgacat cgttgagctg ctgccgggtt ttgctggcat
    17821 taatatcggt ttccacacct tcaactgaag aagtaatccc gttctgatat agctggcgat
    17881 cggtcgcgat aatggtgttc tgctcttttt ctatttgctg caagaccgtg tttaacgccg
    17941 cctgggtttg ccactcccag tacaggcggg ctacgctgcc agccagcaat tggcgggttt
    18001 gctcgcgttc cgccgcccgt gctttaaccg tacccaggcg ggcagtaacc tccgcccgat
    18061 tctttcccca gatatcgaga tgccagcccg ccgttaagcc aaaagtaccg ttggtgtacc
    18121 acgggccggt cgtacctgcg gccggatcgt tcagagcaaa cggccccatt aagccttctg
    18181 ccgacatttt ttgccgctcc atatccgccg aaaagtcgat ctgcggacca tcctgagtgg
    18241 caactgcctt cgcctgggct tcagctagct gaatgcgctg ttcagccacc tgcatatccg
    18301 gtgcgttctg tagtgcattg ttaattaatg aagtgagttg attatcgtga tactccagcc
    18361 accattggct gtctggccaa ccatttttca gcgccgtggg taatgcggtg tcaacttgtg
    18421 cagcgggcgt ttgctggctt aacgcctggc gggtttcatg cataggcgca cacccggcca
    18481 gcatcagtaa cagcggaaaa caggcgatgg ctggataaaa ggaatcacga ttcatggggg
    18541 aataatcagg taagaaaagg tgcgcggaga ttaccgtgtg ttgcgatata ttttttagtt
    18601 tcgcgtggca atacatcagt ggcaataaaa cgacatatcc agaaaaatat acactaagtg
    18661 aatgatatct tccgatttat cttaatcgtt tatggataac ggcaaagggc ttcgtttttt
    18721 cctatactta ttcagcactc acaaataaag gaacgccaat gaaaattata ctctgggctg
    18781 tattgattat tttcctgatt gggctactgg tggtgactgg cgtatttaag atgatatttt
    18841 aaaattaatt aatgtcatca ggtccgaaaa taacgagaat atttcagtct ctcatcctgt
    18901 tgcgctcctg tcatgtgcat tgcttcatat aa
//
LOCUS       DAZFZC010000093        18275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFZC010000093 DAZFZC010000000
VERSION     DAZFZC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.094"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..585
                     /locus_tag="ACHKXP_003823"
                     /pseudo
     CDS             1..585
                     /locus_tag="ACHKXP_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            688..1191
                     /locus_tag="ACHKXP_003824"
     CDS             688..1191
                     /locus_tag="ACHKXP_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO7648748.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1259..2716)
                     /gene="nuoN"
                     /locus_tag="ACHKXP_003825"
     CDS             complement(1259..2716)
                     /gene="nuoN"
                     /locus_tag="ACHKXP_003825"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO7648749.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2723..4252)
                     /gene="nuoM"
                     /locus_tag="ACHKXP_003826"
     CDS             complement(2723..4252)
                     /gene="nuoM"
                     /locus_tag="ACHKXP_003826"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO7648750.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4416..6257)
                     /gene="nuoL"
                     /locus_tag="ACHKXP_003827"
     CDS             complement(4416..6257)
                     /gene="nuoL"
                     /locus_tag="ACHKXP_003827"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO7648751.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQTYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6254..6556)
                     /gene="nuoK"
                     /locus_tag="ACHKXP_003828"
     CDS             complement(6254..6556)
                     /gene="nuoK"
                     /locus_tag="ACHKXP_003828"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO7648752.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6553..7107)
                     /gene="nuoJ"
                     /locus_tag="ACHKXP_003829"
     CDS             complement(6553..7107)
                     /gene="nuoJ"
                     /locus_tag="ACHKXP_003829"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO7648753.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7119..7661)
                     /gene="nuoI"
                     /locus_tag="ACHKXP_003830"
     CDS             complement(7119..7661)
                     /gene="nuoI"
                     /locus_tag="ACHKXP_003830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO7648754.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7676..8653)
                     /gene="nuoH"
                     /locus_tag="ACHKXP_003831"
     CDS             complement(7676..8653)
                     /gene="nuoH"
                     /locus_tag="ACHKXP_003831"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO7648755.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8650..11376)
                     /gene="nuoG"
                     /locus_tag="ACHKXP_003832"
     CDS             complement(8650..11376)
                     /gene="nuoG"
                     /locus_tag="ACHKXP_003832"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO7648756.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGAEVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASAIRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11429..12766)
                     /gene="nuoF"
                     /locus_tag="ACHKXP_003833"
     CDS             complement(11429..12766)
                     /gene="nuoF"
                     /locus_tag="ACHKXP_003833"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO7648757.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12763..13263)
                     /gene="nuoE"
                     /locus_tag="ACHKXP_003834"
     CDS             complement(12763..13263)
                     /gene="nuoE"
                     /locus_tag="ACHKXP_003834"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO7648758.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13266..15068)
                     /gene="nuoC"
                     /locus_tag="ACHKXP_003835"
     CDS             complement(13266..15068)
                     /gene="nuoC"
                     /locus_tag="ACHKXP_003835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO7648759.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRVRTPSF
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15162..15824)
                     /gene="nuoB"
                     /locus_tag="ACHKXP_003836"
     CDS             complement(15162..15824)
                     /gene="nuoB"
                     /locus_tag="ACHKXP_003836"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO7648760.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15840..16283)
                     /gene="nuoA"
                     /locus_tag="ACHKXP_003837"
     CDS             complement(15840..16283)
                     /gene="nuoA"
                     /locus_tag="ACHKXP_003837"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO7648761.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16914..17852)
                     /gene="lrhA"
                     /locus_tag="ACHKXP_003838"
     CDS             complement(16914..17852)
                     /gene="lrhA"
                     /locus_tag="ACHKXP_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO7648762.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSDNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4561 a         5082 c         4719 g         3913 t
ORIGIN      
        1 tttaccaacc ctgggtcact gcattatcgc caacgccccg acacgtaagt cctgagcaat
       61 tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc acgtaatgcc ggcaatattg
      121 ataatctgaa taggtccgta gagtatataa aagagttctt tgtcagtagc ggtgccagag
      181 ttaccgcgca gggcgtcccc ggttggctat tacgactctg cgcctggtag ccaggattac
      241 ccttacccgg caatgtcctg gctttatccc gatcggggag actttattgc cgtggtcggc
      301 agaatgcagg atatcaacgc cgttcgtcag gtaaaagcgg cattgttgtc atcccgggat
      361 ttatctgttt attctatgaa tgccccaggg tttattcccg gtattgattt ctctgaccac
      421 ctgaattatt ggcaacacga tattcccgcc gtaatgatta ctgacaccgc tttttatcgt
      481 aataaacaat accacttgcc cggtgataca gcagacagat tgaattatca gaaaatggct
      541 caggtagtgg atggtgttat aaccttgtta tacaacagta aataataaaa atttctcgag
      601 gcgggtccag aaacagcgac atgtacattt tattttccct cgtctgtttt aagatgcgaa
      661 acataaaatt caatggagga aaaataaatg ggaatgattg gttattttgc agaaattgac
      721 tcagaaaaga ttaaccaact gcttgagtct actgaaaaac ctttgatgga taatatacat
      781 gacacgcttt caggattacg ccgactggat attgataaac gctgggattt tttacacttt
      841 gggctgacag gcacctctgc ttttgatccc gcgaagaatg atccgctgag tcgtgccgtg
      901 cttggtgaac acagcctcga agatggcata gatggcttcc tgggattgac ctggaatcag
      961 gagttagccg cgacaatcga ccgactggaa tcactggatc gaagtgagct gcgtaaacag
     1021 ttttccatta agcgactcaa tgaaatggag atttatccgg gagtgacctt ttcagaagag
     1081 ttagaggggc aactctttgc ctcgatcatg cttgatatgg aaaagctgat tagcgcgtat
     1141 cgacgtatgc tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag
     1201 agcgccaaaa aaaaaccgcc gagcatgtcg gcggctttct gacttacaaa ctaacagatt
     1261 acatcagcgg cattgccaaa cgcacaatac taatcagcgg ttgtggccat acacccagca
     1321 ccagtaccaa cagtgcagaa atcagcacca cgataccgcc cgcgctgtac tgccagtttg
     1381 acggtgcatc gcgacccggt tgttccggtg cgtgcaggta caggctcacc gccacgcgca
     1441 ggtagtagta gaggccgatt gccgaaccga caaccacggc acccaccagc caccacaagt
     1501 gtgcctggac accgaccgcc agcacgtaga acttaccgat aaagcccagc gtcatcggga
     1561 taccggccag agacagcatc atcaccgtca tcactgccgc gaggatcgga cgatgccaga
     1621 acagaccgcg gtaagagaac agggaatcag catccgggcc acgatacggg ctggacatca
     1681 ggctgaccac gccgaacgcg ccgaggctgc tgaacagata accggccagg taaaccccta
     1741 ccgcttccat cgacatctcg cctgtttgca gcgcaatcag cgccaccagc agatagccga
     1801 ggtgagagat agatgagtaa ccgagcagac gtttgatgtt ggtctggctc aacgccatca
     1861 ggttaccgaa gatgatggag gcaaaggcga taatcgccag caccacgcga atcgcttcgc
     1921 tgtcacccac cggtgcgtag aggaacagac gcatcaccac accgaagata gcgattttgc
     1981 tcgccgtcgc caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg tctggcgtcc
     2041 acaggtggaa cggcaccaga gagagtttga agccgaggcc aacaatcatc aggccgaaac
     2101 ctgccagcaa cagcggctcg ttgagcatac cgtcgccaag gtttttaccc aacgcgacaa
     2161 acgacaggtc gccagactgc gcatacacca gcgccatacc aaacagcagg aaagaagacg
     2221 ctgcggcaga aaggatggtg tatttgatac tggcttccag tgaacgtttc tggcggaaag
     2281 cgtaaccgac caggccaaac agcggcaaag agatcagttc gataccgagg aacagagacg
     2341 ccagatggtt ggcattcgcc agcaggatcc cgcccagcgc ggcaattaac accaacaggt
     2401 agaactcatc cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa gtacaggtgg
     2461 cgaggctcgc caacaatacc agcccggtgt aaagcatggc gaaaccatca acgcgcatca
     2521 gcggcgtaac gtccatagcg cccgcctggc caacaaacca gagcgaaacc agcgccgcgt
     2581 taagcccaat aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac gcaatggaga
     2641 gcatcacaac caccaccgtc aagccgacga tcagcaacgg tagcagtgcg atcaggtttt
     2701 gtggagttat tgtcatggcg atttacggcc ttgtagtagt aacggaatta acaaaccact
     2761 gctggatatt gccaatcgcg gagtgcgagg tatccagaat cggctgcgga tagaagccca
     2821 gcagtaccag cagcaccacc agcaacagga tcataaacag ctcacgcagc gacatccctg
     2881 gcagttcctg gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga tgtaacatcg
     2941 ccagcgaata aacagatgca aagaccagcc caaaggtaga gataacggta atcaccggta
     3001 caacctggaa gctgccgaac agaatcataa attcgccgac gaagttaccg gtgccaggca
     3061 tcccaagcgt tgccaccgca aagaacagcg acagtgctgg cagccatttc atcttgctcc
     3121 acagaccgcc catcatgcgc atgtcgcggg tatggatacg ttcataaagc tgaccacaaa
     3181 gaataaacag acccgccgcc gacaaaccgt gcgcaatcat ctggattacc gcgccctggt
     3241 aggccaactg gctgccggtg tagatagcaa tcagcacgaa gcccatgtgg gaaaccgagg
     3301 tgtaggcgat cagacgtttg atatcggtct gggcgaaggc catccacgca ccgtagaaga
     3361 tgccgataac acccagccac atagcaattg gcgcgaactc tgccgacgcg ttcgggaaca
     3421 gcggcaggga gaaacgcagc aaaccgtaag cggcagtttt cagcaagatc cccgcgaggt
     3481 caacggaacc ggcggtcgga gcctgggagt gcgcatccgg cagccagcca tgcagcggaa
     3541 ccaccggcat tttgactgcg aaggcgatga agaagcccag cattaacagg tattccacac
     3601 cactggacat tggcgtattc agcagctctt catagttgaa ggtccagacg ccggtcgcat
     3661 tgtagtgaac aaaaaccagc gccaggatgg caatcaacat caccaggcca ctcgcctggg
     3721 tgtaaatgaa gaacttggtt gccgccgtga tacgcgtttt accgtcagag gctttatgcc
     3781 cccacagtgc gatcaggaag tacatcggca ccagcatcat ttcccagaag aagaagaaca
     3841 ggaacatgtc gatggcaagg aacacgccga taacgccgcc caggatccac atcaggttga
     3901 ggtggaagaa gccctgatat ttttcgatct ctttccacga acatagtacc gccagcacac
     3961 cgagcagacc ggtcagcacg accatcagca gcgacagccc gtcaatggcg agatgaatag
     4021 agataccaaa acgcgggatc cacggcatgt cgaattcaga ctgccactgc ggaattccgg
     4081 cggattgcgt cagtgaataa ccgccctgca accacagttg cagcgacagc gccagcgtca
     4141 atcccatggt gatcagcgcg atccagcgtg gcaccttgac gccaaagcgt tcggtctgcc
     4201 agcacaggaa gccgccaata aagggaatta atattagcca gggtagtaac atggcgatct
     4261 ttattccttg taaaagtccc gtcaggaccg gattttcaac gaattctcac gacagaaaat
     4321 tctcttcgga ctgggggtag tgtcggatgc accgcgaagg cggcttatcc gacctacgtt
     4381 cttatatgcc caggggcaat cccacaatcc ttaactcaac gcagtaccat caacagtgcc
     4441 agcaccacga ccgcaccgat gctcatggat gccacatacc agcgcagata gccgttctca
     4501 cttaacagca gacctttacc tgcaaagcgg gaaaggacag ccgggatgtt catcattgag
     4561 ttcagcggat cgcgtttcag caaccaggca atacccagga acggcttgac gaacactttg
     4621 tcatacagcc agtcaaatcc ccaggcgttg taccaccagg tgcccagcag acggcccggc
     4681 gcactgttgg cgatggaggt caccagagta cgtttaccca gccacagcca ggctgccagc
     4741 agaatgccga ccaccgcgac cacgccagag gtaatttcca gggtcaacat gctgccgtgc
     4801 gccagttccg tcgtttgcgg aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg
     4861 aaggtggaaa ggatcagcag cacaatcagc ggcaggctgt gagttacccc tttcacggcg
     4921 tgagcgtgaa tttgttcttt tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc
     4981 gaggtcataa acgcaccgac cagacctgcc accatcagat tgatatgacc attcgccatc
     5041 gcacccgcga ggatctcatc cttactgaag aagcccgcag tgaccagcgg tagtgccgac
     5101 agtgctgcgc cgcccaccag gaagcagaga taaaccagcg gaatagattt acgcagaccg
     5161 cccatcttga agatgttctg ttcgtgatgg caggccagaa tgacggaacc ggatgccagg
     5221 aacagcagcg ctttaaagaa cgcgtgggtc atcaagtgga aaatcgccgc atcccatgcc
     5281 tgcacgccaa gcgcgaggaa catgtagcca atctggctca tggtagagta agcgagaaca
     5341 cgtttgatgt cggtctgtac cagcgcggca aaaccggcca gcagcagcgt aaccgccccg
     5401 acaataccca ccagatgcag aacttccggc gtcatcagga acaggccgtg ggtacgggcg
     5461 atcaggtaga cacccgcggt taccatggtt gcggcgtgga tcagcgcgga gacaggcgtc
     5521 gggcccgcca tcgcgtcggc aagccatgtc tgcaacggca actgcgcaga tttaccgacc
     5581 gcaccgccca gcagcatcag cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc
     5641 gctggtgcca gttccaccat ttcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg
     5701 aacagtgcga aagcgaggaa cacgtcaccc acacgggtca cgacgaacgc tttcattgcc
     5761 gctgcgccat tcttcggatc ggtgtaatag aacccgatca gcagatagga gcacaggccc
     5821 acgccttccc agccgaggta catcagcagc aggttgtcgg caagcaccag aaccaccatg
     5881 ctggcgatga acaggttggt gtaagcgaag aagcgagagt agccctcttc accgcgcata
     5941 taccaggagg cgtacatgtg aataaggaaa cccacaccag tgaccaccga gagcatggtc
     6001 agcgacaggc cgtccagcac caggttaaaa ccgatgttaa agtcgcctac cgacatccac
     6061 gtccacagcg gctggctgta tgtctgctcg ccgttagcga agaaatcaac gccgataaag
     6121 gcggttacca gcgccgccag gcccacagag cctacgccga cgatcgccga gacgttttca
     6181 gaccagcgcc cacgggagaa tgccagcagg acgaagccaa tcaatggcaa aataatggtt
     6241 aaggcaagca tgttcatccg cgcatctcac ttactgaatc gatgttcagg ttctggcgac
     6301 gacggtgaag ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc gcgaggctga
     6361 tggcgagaat gtacatcacc tgaccgtcgg tctggcccca gtagcttccg gcgaccacga
     6421 aggccagcgc ggaggcgtta atcatgattt ccagaccaat caacataaac agcagattgc
     6481 gacggataac cagaccggtt aagccaagaa cgaataagat tgccgcgagg atcagtccat
     6541 gttgtaaggg gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt
     6601 gctcagcact tcacccgcac gctcttcacg accgacgtgg aaggccacaa ccagacctgc
     6661 gagcagcagc atagaagcca gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc
     6721 cactgcttta gcactgattg gcgtaccgtc gataccctga tcgttaacac cgaggatggc
     6781 gtaaacaatc accaccagca tgatggccga caaaattgcc ggaccaatcc acacctgcgg
     6841 tttcagccac tggcgttcct gttcgatttc tgaaccgccc aggttgagca tcatcaccac
     6901 gaacacgaac agcaccataa tggcacccgc gtagacgata atttccagcg caccggcgaa
     6961 gtaagcgccc agtgagaaga acaccccgga aatcgccagc agcgaaataa tcaggtacag
     7021 cagtgcgtgt accggattgg tatgggtgat cactcgcaag gtcgcaagta tggctatcag
     7081 gccacagata taaaaagcga actccattgc ccctctcctt acggtaacag gctcttgacg
     7141 tcgataggct tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat tgccatacct
     7201 gccatccggt agaagttata ttccgggtat ttgcccggac cggagatcag cagatcctct
     7261 ttctcgtaaa ccagatcctg gcgcttgtat tcccccattt cgaaatccgg ggttaactga
     7321 atcgccgtgg tcggacaggc ttcttcgcac agaccacaga aaatgcagcg tgagaagttg
     7381 atgcggaaaa attccgggta ccagcgaccg tctttggttt ctgctttttg cagcgagata
     7441 cagccgaccg ggcaggctac cgcgcagagg ttacaggcta cgcaacgctc ttcgccgtcc
     7501 gggtcgcggg tcagaacgat acgaccacga taacggggcg gcagatagac cggctcttcc
     7561 gggtacattc gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca gatactacga
     7621 acctgggtgc cgaaacctac taacaattct tttaaggtca tggtcttatt gccccttatt
     7681 gcgcctgcca gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc
     7741 agattttcca gccgaaggac attacctggt cataacgcgg acgcggtaac gacgcacgaa
     7801 tcaaaatgaa catcatcata aagaacgcgg ttttcagcgc gaaccagatg aatggcggta
     7861 acaacgggcc ttgccagcca ccgaagaaca gcgtcaccat caatgcagag atggtcacaa
     7921 tcccgatgta ctcacccacg aagaacagac cgaacttcat accggaatat tcaatgtggt
     7981 aaccatccgc cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg tgacataccg
     8041 ccacgcccgc gatggcaaag gtaataaaac caaagaattg cgggataacg ttccacacat
     8101 gcgcctggct gttgacgatg tcggtcatgt tgaatgaacc ggcctgcgcc accacgccca
     8161 tcaaggaaag cccgaggaac acttcgtagc tcagggtctg cgcagaagca cgcatcgcac
     8221 ccagcaacga gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca
     8281 gacctgccat catcaggaag aacaaaatcc cgatgttcag gtcggcaacc acccaacccg
     8341 gactgactgg cacaatcgca aaggccagca gcagcgaggt aaaggcaatc atcggtgcca
     8401 gggtaaagat gacgcgatcc gagaatttcg ggatccagtc ttctttaaag aacattttga
     8461 tcatgtccgc aaccagctgg agcgaaccgc cccagccaac acggttaggt ccgtaacggt
     8521 tctggaacag acccagcagg cgacgttcgc caaagctcat gaatgccccg caggtgacaa
     8581 ccaccagcag gatcaccacc gctttgagga tggtcagcag gatctcaatc agttccggtg
     8641 atatccaact cattgttgtg cctccttgag atcctcaaga tgcgcgccag ccagcaccgg
     8701 agcaatgccg gacataccca tcggcaagcc cacctgccct gccgtcagtc cttcggcgat
     8761 ttcaaccggc agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg tacctgcgtt
     8821 cacacccaac ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg gcatacggct
     8881 ctggaagacc ggagcacgct gtgacaattc atcgctgcca aacaggtggt aatacggcgc
     8941 gatacgccat ttcccgtcct gcggctggaa gcgtgccggt acgctggtga agtaatccag
     9001 accattttcg ctggtttcaa acagacgcac gcccggatcg ccaaagcgca gtttgccgcc
     9061 cacttcgtcc tggaatttgt tccacgcctg cggggagttc cagcccggcg cccaggcaaa
     9121 cggcacttgc gaacggtgcg cagtcggctg gttgttacct tccatcgaga aggtgaacat
     9181 ggtgtcaata tcctgcggct gacgcggctc atgaacgctg atattggcgc gcatggcggt
     9241 acgaccgctg taacggtgcg gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc
     9301 atccggcgca gcatctttga tacctgccag ttccgggatt ttcgccacaa cagcgtcaat
     9361 cacatggtcg agctgcgtcc agtccacttc acggctcagc agggtgctgt gcagcgagtg
     9421 taaccagcgc cagctttcca gcatgacagt tttgctgtcg taataagcag gatcgtaaac
     9481 ctggaagaaa cgttgggcgc ggccttcgtt gttgatcacc gtaccgtcgc tttcagcaaa
     9541 gctggcagca gaaagtacca gatgggcgtt ttccataatc gctgtgcgtt gatgatcaac
     9601 caccatcacc agcggtgctt tagccagcgc agcattcacg cggatagcag aagcgtgacg
     9661 atgcagatcg ttttccaaca ccaccaccgc gtcggcgcgt ccggtttcca gttcggttaa
     9721 cgcttcttca agcgaaccgc cacccataat gcccagcccc atgctgttga cggaacgggc
     9781 aatcatggtg ataccgacct cagcgccgcg acctttcagg gctttcgcga cgttagccgc
     9841 cgcctgaatc acctctaagc taccggcgtt cgtcccggag ataatcaacg gtttcttcgc
     9901 acctgccagt gcctgcacga tgacgtcgat tttgctttgc agctcaggtt cgataccgtc
     9961 aaccgctggt gcagagttat ccagcgcatg ggcgatggca aaacctaaac gcgcctgatc
    10021 ttcaaccggt gcgcggtaag tccacgccgc gatatcatcc agacgggtgt catcaacgtt
    10081 ggtaacaaac agcggatgct tcgcacgttg accgatgttg aggattgccg caatctgcca
    10141 gtcagccact ttctgtgctg ccgccatttc gcgcgcttta cctttcacag cctgacgcac
    10201 tgccagcgcg acgcgcgcgc cggtctgggt aacgtcttcg cccagcacca gtaccgcatc
    10261 gtaagattcg atttcgcgca gagccggagt ataaatgccg ccttcacgca gcactttcag
    10321 cgccagttgc agacgttcct gctcaccgtg agcgataccg gtgtagaagt tttcttcgcc
    10381 caccagttca cgcagcgcaa agttgctttc cacgctggca cgcggagaac caataccgat
    10441 cactttcttc gactgacgca gaatatctgc cgcgccctgc attgcctgtt cggcgttgag
    10501 ggtaatgaaa tcatcgccac gacgctgtac tggctgacgc ggacgatcct tcaggttgac
    10561 gtaaccgtaa ccgaaacgac cacggtcgca gaggaagtag tggtttaccg taccgttgta
    10621 acggttttcg atacgacgca gttcgccgta acgttcaccg gggctgatgt tacagccgat
    10681 ggaacattgc tggcagatgc tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc
    10741 ggagtgcgtt ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt taccggaaaa
    10801 ttcgctttcc agcgtgccgt cttccgggcg accgaagtag acgttgtcgt gcgcaccgta
    10861 aacgcccaga tctgtaccgt cagcgtaatc tttgtagtaa cgcacacagc ggtagcaggc
    10921 gatgcagcgg ttcatttcgt gagagatgaa tggccccaaa tcctgattac ggtgggtacg
    10981 tttggtgaaa cggtagcgac ggaagctgtg tccggtcatc acagtcatat cctgaagatg
    11041 gcagttaccg ccctcttcac ataccggaca gtcgtgcggg tggttggtca tcaaccactc
    11101 gaccacgctt tcacggaact gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc
    11161 ggaagccggt gtcatacagg acatcaccag gcgaccacgc gtgtcttccg cgttttggta
    11221 ttgcttcacc gcacactggc ggcaagcacc gacacttccc agcgccggat gccagcaaaa
    11281 gtaaggaata tcaaggccca gagacagaca agcttccagc aggttgtccg ctccgttgac
    11341 ctcgtattct ttgccgtcta catgaattgt agccattagc atgcttccag ttttctcagt
    11401 cagagactga gcgttaatcg aaattcggtt accagcgctc tttcagcagg ttcggctgaa
    11461 tcccattaat caaatgggta ttgctgaacg gctgtttgat tcccgcctca aattcttcgc
    11521 ggaaatattt gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag
    11581 ttttacccgg gcctaagaat cgacacagtt gctcaagtgt ttcgatatcg cccggctgac
    11641 cttcaccacg ctccagcgca cgcagaattt tcacgctcca cggcagaccg tcgcggcacg
    11701 gcgtacacca gccgcaggac tcacgggcga aaaactcttc caggttacgc accagcgaca
    11761 ccatgttgat ctcatggtca accgccatcg ccagcgccgt acccagacgg ctgcccgctt
    11821 taccgatact ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg aagtcagtcc
    11881 ccgcgccgcc tggctgccag gctttaaatt tcagaccatc acgcatacca ccggcgtaat
    11941 cttcgaggat ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc ggatttttca
    12001 cccgaccgga gaagcccatc agcttggtgc cagcatcttt acttttcgag atgttctgat
    12061 accactccac gccgttagcg aggatcgccg gaacgttaca cagggtttcg acgttgttga
    12121 cacaggtcgg tttgccccat acgccggagg ttgccgggaa ggggggtttc gagcgtgggt
    12181 tagcacgacg cccttccagg gagttgatta acgctgtttc ttccccgcag atgtagcgcc
    12241 ctgccccggt atggacgaac agttcgaagt cgaaacctgt tcccataatg tttttgccaa
    12301 gcagacccgc ttcggtggct tcggcaatgg cacggcgcag attaactgcc gcttcgatat
    12361 attcgccacg caggaagatg tagccacggt aagctttcag cgcaaacgcg gagatgagca
    12421 taccttccac cagcaggtgc ggcagttgct ccatcaacag gcggtcttta taggtgcccg
    12481 gctccatttc atcggcatta cacagcaggt aacggatgtt catggattcg tctttcggca
    12541 tcaggctcca tttcaggcca gtcgagaagc ccgcgccgcc gcgccctttc agaccagcgt
    12601 cttttacctg attaacgatt tcgtccggag acagcccggt cagcgcctta cgcgcgcctt
    12661 cgtaaccgtt tttgctgcgg tattcgtcca gccacactgg ctgtttgtca tcgcgcagac
    12721 gccaggtcag cggatgcgtt tcgggagtac ggataatgtt tttcatttat accgctccag
    12781 cagttcaggg atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt
    12841 tggcccttta tcacagttcc ccaggcagca agttggcagc agcgtaaagc ggccatcaaa
    12901 tgtcgtttgc cctggtttga tgttcagctt tttctcgagc gccgcctgaa taccctgata
    12961 accgttgata tgacagacca cgctgtcaca ataacggatc acatggcgac caaccggctg
    13021 gcggaagatc tgactgtaga acgttgccac accttcgacg tcgcttgccg gaatacccag
    13081 cacatcggcg atcgcgtgga tcgcaccatc cggcacccag ccacgctgct tctgaacgat
    13141 tttcagcgct tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcgat
    13201 cgcttcacgc tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg
    13261 cataattagc ggtccacatc tgacataaca aaatcgatac tgcccagata aacaatcagg
    13321 tcagacacca ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc aaagctcggg
    13381 gtacgaacac gggtgcggta actcatggtg ctgccgtcgc tggtcaggta gtaactgttg
    13441 atccctttgg tcgcctcaat catctggaaa gattcattgg caggcatcac cggaccccac
    13501 gacacttgca ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg ctctttcggc
    13561 ggcggcgtgg tcagcgggtg atccgctttg aacgggcctt ccggcatgtt gttgaggcac
    13621 tgctcaagaa tgcgcagact ctggcgcagc tcttccactt taagcattac gcgggtgtag
    13681 cagtcagaaa cgccaccacc caccgggatt tcaaagtcga agttttcata gccagaataa
    13741 ggacgcgcct tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc gccagtggtg
    13801 ccccactcca gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg acctttcaga
    13861 atggtgtttt gcagcgccgc tttctcgtaa gacgccagac gtttcggcat ccagtcgagg
    13921 aactcacgca gcaggcgatc ccagccgcgc ggcaggtcgt gcgctacgcc gccaatacgg
    13981 aaccacgccg ggtgcatacg gaaaccagtg attgcttcca ccagatcgta aattttctga
    14041 cgatcggtaa aggcgaagaa cactggcgtc attgcgccga cgtcctgaat aaaggtcgag
    14101 atatacagca ggtgactgtt gatgcggaac agttcggaga gcataacgcg aatgacgtta
    14161 acgcgatccg gcacggtgat cccggccagt ttctctaccg ccagcacgta aggcatttcg
    14221 ttaacgcagc cgccgaggta ttcgatacgg tcagtatacg gaatgtagct gtgccaggac
    14281 tggcgttcgc ccattttctc cgcaccacgg tggtggtaac cgatgtctgg tacgcagtcg
    14341 acaatctctt cgccatcgag ttgcaaaacg atacggaaag ccccgtgcgc cgacgggtgg
    14401 ttcggaccga ggttgaggaa catgaagtcc tcgttttcgg tgccgcgctt catcccccac
    14461 tcttccggtt tgaaggtcag ggcttccatc tccagatcct gtttggcttt ggtcagctca
    14521 aacggcgaga attcggtagc gcgcgccgga taatctttac gcagcgggtg acctttccag
    14581 gtttgcggca tcatgatgcg tcgcaggttc gggtgaccgt cgaaagtaat gccaaacaga
    14641 tcccaggttt cacgctcata ccagttagcg ttcgggaaca gtttggtgaa ggtcggtacg
    14701 tgcaggtcgt tttctgccag cgccaccttc agcatgatgt cgcggttacg atcgatagaa
    14761 atcagatggt agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg tgtgcgcaga
    14821 cgttcgtcca tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag tttctttaag
    14881 aaatcgccaa cttccagtaa ttgttcacgc ttgatccaca caacgggaac cccggtgcga
    14941 gtcgcctgaa cagtaaaggc atccggccca aaacggttgc gcagttcgcc aatcaccgga
    15001 tcatcaagat gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc ggtcatattg
    15061 ttcaccattg caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg aaatagattt
    15121 aggaataagc gaagaaaaat ccctttgccg acaggcgcaa attaaatctc gtcaggtgta
    15181 cgcaggttag ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg
    15241 gcgcgataaa cgccctgatc gccaaccacc caggagagcg gacgacgttc tttgccgata
    15301 gattcctgca acagcatcag tgcctgcatg tacgcttcag gacgcggcgg gcagcccggg
    15361 atatacacat caaccgggat gaatttatcg acgccctgca caacggaata aatatcgtac
    15421 ataccaccag agttggcaca ggcacccatt gagataaccc attttggttc cagcatctgg
    15481 tcatacagac gctgaataac cggtgccatt ttggtaaagc aggttcctgc aaccaccatc
    15541 aggtcagcct gacgcggcga agcacgcaat acttctgcgc caaaacgcgc cacgtcatgc
    15601 accgcggtaa acgaagtcac catctcaacg taacagcagg aaagaccgaa gttatacggc
    15661 caaattgagt ttttacgacc ccagttaacc atgtcattga gcttgcccat aaacacgttt
    15721 ttgttaactt cttgctccag agggtcggtt acgatctcct gcttttgcag ggggtaacgg
    15781 tcgttctcac cgttgggatc tatgcgggtg agcgtataat ccatcttaat gcctcgcggt
    15841 tagcgttgac gattagcgat actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc
    15901 gcgggcgtcc agtccagcgc gccaatacgc accagataaa ccagacctgc cagtaacaca
    15961 aaaataaaaa ttgcagcttc cacaaagcct acccagccgc tttcgcggat agaggttgac
    16021 catgcgaaca gatacagcgc ttcaacgtcg aagataacga agaacatggc caccagataa
    16081 aacttggcgg acaggcgtaa gcgggcggag ccgaccgagt cgataccgga ttcaaacggc
    16141 acgtttttcg acctcgcgcg tgcgcgaccg cctaaaaacc aaccgcctac cagcatcagg
    16201 caacacaggc caatggcaac gataagaaag atagcgaatg cccagtgatg agcgatgact
    16261 tcagtggatg ttgacatact cattgcttac tcatcaaaag tagcgccaga ttcactgctc
    16321 ttcacggcag atggacgcca catcgattca tggggaggaa taaaaaaaac cttacaatca
    16381 ctgtagaaat tcttttatac agctaattga tgtggtcttt tactcctttc tataaccttt
    16441 tgtcaacttt aacaaaagtt tcttcacatt agtttacata atatcaacac cattagcatt
    16501 taatgccctt tcaccccaga tccttgacga ctccaggata attagatgtt gttgaatcgt
    16561 gtccgttgtg aagcaatgga aaaaatacgg gtctattttg acaggaattt gtgtcgattc
    16621 ctccccccaa aagagagtat tttcttgatc tgtgacacgc ttttgtcatt ccataacaaa
    16681 aacgcagcaa caaatttacg tattttttaa catcattgta gcaggtgatt tttttcaggc
    16741 gattatttgt gcgttcggga cgtgaatctc tggtgattga aaaatgaaca gttttgtgcg
    16801 ttctgcacta tgcgaggaag gatttttact aaaaaaaagc cgctggggtt taaaacaccc
    16861 ccagcggctc gttttttaca ctattgtctc aggaattatc tatcgtccgt cgattactcg
    16921 atatcccttt caatcaacaa tgaatcatcc ccttccggag cagacattgg gctgtattgc
    16981 cacggattat ggtagctctc catcgcctga tagatcacct gggccagctc attatcactg
    17041 gacgggtcat agcacagcaa atattcggta tcaggtagcg gcggtaagcc atctactcca
    17101 ctcagcacac gcaggtccgg gctcatcatt tcaaccggcc ttgccgtcac gccaagaccg
    17161 gctttcactg ccgcacgaac ggccggaagc gtcgaggcga cataagccag tcgccatgga
    17221 atatctgctt tattaagcgt cgccagcacc atatcgcgaa acggacttgg atcatccagc
    17281 aatacaagag ggatcggctc acctttttgc aatacgtatt ccgctgcgca gtaccagtgt
    17341 gttggcgagg tacgcaggtt caatgcctta aaggtagaag gacgatgggt ggttaccatc
    17401 aaatccactt cctgggattc cagcatttct gccatatagg cattacgttt aacgcggaca
    17461 tccagcgcta atttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta
    17521 tcggcagatt catctgaagc accgatagtt aacacgccct gaagattact gaacattaat
    17581 gatgagcagg cctcatcatt aaaacgcagg attttcctgg cgtaaccaag aagttgaatg
    17641 ccatgttcag ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacgagt
    17701 tgttccagac gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct
    17761 gcggcagcaa aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg
    17821 aggttaatta tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact
    17881 gagctggtta atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg
    17941 cagtttcatc cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagcga
    18001 ctcacattgt tccgttataa tgcctgaagt agatcacaga atatatcttc agggatcgca
    18061 tatctattaa gttactcact cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa
    18121 atgtgagtcc ttgttccact ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac
    18181 agcaatggtg aggcggcatc aagagcaggg atccgtcatt tatgcgagca ttttacccca
    18241 aaaacctttt atttataagg gtcattgcga attat
//
LOCUS       DAZFZC010000094        18255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFZC010000094 DAZFZC010000000
VERSION     DAZFZC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.095"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1015)
                     /locus_tag="ACHKXP_003839"
                     /pseudo
     CDS             complement(1..1015)
                     /locus_tag="ACHKXP_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1642..1935
                     /gene="iss"
                     /locus_tag="ACHKXP_003840"
     CDS             1642..1935
                     /gene="iss"
                     /locus_tag="ACHKXP_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO7648763.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSQ"
     gene            complement(1993..2168)
                     /locus_tag="ACHKXP_003841"
                     /pseudo
     CDS             complement(1993..2168)
                     /locus_tag="ACHKXP_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            2746..3770
                     /locus_tag="ACHKXP_003842"
                     /pseudo
     CDS             2746..3770
                     /locus_tag="ACHKXP_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178356.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            5081..6196
                     /gene="iroB"
                     /locus_tag="ACHKXP_003843"
     CDS             5081..6196
                     /gene="iroB"
                     /locus_tag="ACHKXP_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HFO7648764.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            6336..9995
                     /gene="iroC"
                     /locus_tag="ACHKXP_003844"
     CDS             6336..9995
                     /gene="iroC"
                     /locus_tag="ACHKXP_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HFO7648765.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSNISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSIIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLPAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            10099..11328
                     /gene="iroD"
                     /locus_tag="ACHKXP_003845"
     CDS             10099..11328
                     /gene="iroD"
                     /locus_tag="ACHKXP_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HFO7648766.1"
                     /translation="MLNMQQHLSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            11413..12369
                     /gene="iroE"
                     /locus_tag="ACHKXP_003846"
     CDS             11413..12369
                     /gene="iroE"
                     /locus_tag="ACHKXP_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HFO7648767.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(12414..14591)
                     /gene="iroN"
                     /locus_tag="ACHKXP_003847"
     CDS             complement(12414..14591)
                     /gene="iroN"
                     /locus_tag="ACHKXP_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HFO7648768.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKSSDDDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDSDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(15446..16480)
                     /locus_tag="ACHKXP_003848"
     CDS             complement(15446..16480)
                     /locus_tag="ACHKXP_003848"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFO7648769.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKMYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAIIAAREQHIVYMTSLTNS
                     ISTLLTDGNPHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     KRQISVARQVIDNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQSVTDECISWQQTE
                     QLLSTLAAQL"
     gene            16661..16962
                     /locus_tag="ACHKXP_003849"
                     /pseudo
     CDS             16661..16962
                     /locus_tag="ACHKXP_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17040..17333)
                     /locus_tag="ACHKXP_003850"
     CDS             complement(17040..17333)
                     /locus_tag="ACHKXP_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO7648770.1"
                     /translation="MWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTLLMGGLWGISW
                     GCAMWFMYWGPSGMVAGEAIIISITSGFLFGLLMASFHWWRRKVNRLPPWNDV"
     gene            complement(17501..17626)
                     /gene="yacB"
                     /locus_tag="ACHKXP_003851"
                     /pseudo
     CDS             complement(17501..17626)
                     /gene="yacB"
                     /locus_tag="ACHKXP_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            complement(17626..17894)
                     /gene="yacA"
                     /locus_tag="ACHKXP_003852"
                     /pseudo
     CDS             complement(17626..17894)
                     /gene="yacA"
                     /locus_tag="ACHKXP_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
BASE COUNT         4463 a         4672 c         4512 g         4608 t
ORIGIN      
        1 ccgttactct ccggactccg catcgccgtg ttcttcggtt caagccccaa catccgggca
       61 aactgccgtg tttcattagc ccggtagcat gaaccattat ccgtcagcca ctctactgga
      121 gacgccggaa gctcgttgcc gaagcggcgt tccaccgctc ccagcatgac gtcctgtact
      181 gtttcactgt tgaagccgcc cgtagtaacc gcccagtgca gtgcctcacg gtcacagcag
      241 tccagcgcga acgtgactcg cagtttttct ccgttatcac agcggaactc gaacccgtca
      301 gagcaccatc gctgattact ttctttcaca gccactctgc ctgtatgtgc ccgtttcgat
      361 ggcggtacag caggttttcg ctcaagcaac agcgcattct ggcgcatgat ccggtaaaca
      421 cgtttggcat tgatcgcagg cataccatca agttctgcct gtctgcgaag cagcgcccat
      481 acccgacgat aaccatacgt gggcagctct ccgataacat ggtgtatacg gagaagcaca
      541 tccgtatcat cagtgtgacg actgcggcgg ccatccatcc agtcatcggt tcgtctgaga
      601 atgacgtgca actgcgcacg cgacacccgg agacaacggc tgactaagct tactccccat
      661 ccccgggcaa taagggcgcg tgcgctatcc acttttttgc ccgtccatat tcaacggctt
      721 ctttgaggag ttcattttcc atcgttttct tgccgagcag gcgctggagt tctttaatct
      781 gcttcatggc ggcagcaagt tcagaggcag gaacaacctg ttctccggcg gcgacagcag
      841 taagacttcc ttcctggtat tgcttacacc agagaaataa ctggctggct gctacaccat
      901 gttgccgggc aacgagggag accgtcatcc ccggttcaaa gctctgctga acaattgcga
      961 tcttttcctg tgtggtacgc cgtctgcgtt tctccggccc taagacatca atcatctgtt
     1021 ctccaatgac tagtctaaaa actagtatta agactatcac ttatttaagt gatactggtt
     1081 gtctggagat tcagggggcc agtctaaggt gcacgaatcc tagaggcaga atgtttctcc
     1141 acatgcttgt ggagaaaccg ccgcattttg cccgttctgt acgatacacc gtagccagag
     1201 ataagatatt ctgttgctgt atgctacata cattacacta tacgaaatca ggtctgttca
     1261 atcagtaagg aaataaacgg acaaacgtta tgtaacccag acagagatat attgtctggg
     1321 aaaatagtgg cttttgtggc gaaaactagt aaaacagcaa cccgaaccac ttgatgtgca
     1381 tcgtttttga ttattcccgt atactcttgc agaaggagtt ctccgtcggg ctactgtcat
     1441 ggttaatgcg gggaatatgg cgacaataca acacacctaa aagagtaatg gacagatgaa
     1501 gcggtttatt catttcccat gattctgagt acctaccaag tctgagtaac cacttttata
     1561 cttttaattt tcgttcattt agctatcgtt taattattat cacataggat tctgccgttt
     1621 ttaacaatgc aggataataa gatgaaaaaa atgttatttt ctgccgctct ggcaatgctt
     1681 attacaggat gtgctcaaca aacgtttact gttggaaaca aaccgacagc agtaacacca
     1741 aaggaaacca tcactcatca tttcttcgtt tcgggaattg gacaagagaa aactgttgat
     1801 gcagccaaaa tttgtggcgg tgcagaaaat gttgttaaaa cagaaactca gcaaacattc
     1861 gtaaacggat tgctcggttt tatcactttt ggcatctata ctccgctgga agcccgggta
     1921 tattgctcac aatagttgcc catcgatatg gggagctcat ctgcactgtt cattaatata
     1981 cttctgggct ccctacagtt gtttttgcat agtgataagc ctctctctga gggaggaaat
     2041 aatcctgttc agcgatgtct gccagtcggg ggggctgcat tatccacgcc cgaggcggtg
     2101 gtggcttcac gcggggatgg gcagattgat ctgatatgca accgacgacg accagcggca
     2161 acatcatcac gcagagcttc attttcagat ttgggccacc ttttgatttc tcgtttaact
     2221 tttgcagtca ccagactgtg atttatttta acaaatcaat agctttcata aaattctcga
     2281 agctgaaagc ttaactgaac caaccagcct ggctggtgtt ttctaaagac aaaaaggcca
     2341 tcagcaaaac actgatagtc tgaatcacct ttatcaatca tgtatgaaga aaatatgcgc
     2401 aattagttga cagtgattat cattttcatt aaaaagagcg cgtagcattc ttatttcatg
     2461 aggaaattta cccgccaaca acctgagtag cgaaagctgt tcatccccaa tatttttgct
     2521 ggcgggttct tttttctact gtcctcctcc ccagaggaag cattcccatt aatcattact
     2581 ggcgaggagg caagagaaaa agtcggttct ttgtccctaa ataacagcat catttcagtc
     2641 catgcaaaat ctgatttacc agctcacata ataacctgat gatacacagt gtaagttcac
     2701 agagatattg caattgcctc cggataagta aggggagatt gcactatgca aatgcagcac
     2761 ctgaggttgg ctatcctaag cagccgatta tgcgttgagg ctttcagtga tggctgatat
     2821 agcaacaatg agaatgaagg ctctgcactt ctgggataaa cacggtattt ctgcagcttc
     2881 tgaagccttt ggcgtatcct gccgcacgct ttactggtgg cgtcagttac tgatcaaggg
     2941 aggacctgag gggctaatcc cgcacagtaa ggcacctctg gtgcgacgaa aaaagcactg
     3001 gcatcccgat gtgctgaaag agattcgacg cctcaggaca gagctgccga acctcggtaa
     3061 agagcagatt tttgttcgcc tgaagccctg gtgcgcataa cgccatcaga cctgcccgag
     3121 tgtttccacc atcggcagaa tgatcgccac cgcacacgat aaaatgcgga tgataccagt
     3181 acgtctgagc tcgcggggta aggctctgct tgtcaaaaag cgaaccacca cccccccgca
     3241 ggccaaagca ctaccgtccg gtaaagacag gagagctcat tgggatggac gccattgagc
     3301 tcagaatggg tgaaatgcgt cgctatatca ttaccatgat cgacgaacac agcgattatg
     3361 tactggcgct ggctgtgccg tcgctcaaca gtgatatcgt caatcacttt ttcagccgtg
     3421 cagcccggct gttccctgtc ggtatcagcc agatcatcac agataacggg aaagagttcc
     3481 tgggaagctt tgacaaaacg ctgcaggaag ccgccatcaa acacctctgg acctatccct
     3541 acacgccaaa aatgaacgct atctgtgaac gttttaaccg ggcattaaga gagcaattca
     3601 ttgaatttaa tgaaatttta ctctttgagg atctggcgtt gtttaacctg aaactggcgg
     3661 aatatctggc gctgtataac agcaaaagac tccacaaggc gctcgcacta acgacgcccg
     3721 tggaatatat tttaaaagag aacaaaaatt gcaatatgtg gtggacccat acacagcaaa
     3781 cgcacaggcc gccccggttg ggtagatatg tgggatggct ggttcagact tcaggatatg
     3841 gttgaggctg gtgatgaagt ctctttatca ggagatctga tcaagagaca gccgcgcagg
     3901 gggattcact atgctggatt cttttaacaa attctccgct aaccaaaaaa tagacttttt
     3961 gcattcgtaa atgcgttaag agataatttt taaagttcca tcaatctccc ggccagttcg
     4021 tctcgcggca ttactcacta tccgcacaac ttcagtaaga tcatgacttt ttcaatcgtt
     4081 atctgttgac caagctttga aaccactagc gtcccgtcaa cctgacctgg aatcgtctag
     4141 aaccgcccat agttgtgaat tggctagagt gtgatggaaa gcaacgctaa acgacctagt
     4201 cacacattct cttttcccta acaatgacca tttagtgaaa taacgtatcc aaaatgatga
     4261 cgtgaaagcc tgatactggc agcgaaaagc cgaaaaaact cacctggtca ggagcaaaaa
     4321 tcggattatt cgccaacagc gtaatatcag cagtagttct cgacacgctt gcgctataga
     4381 cagaggatga aacgctgctg gtcggagttc tctatacatt tttggcgacg gcagaaccgc
     4441 tcaccaatgc cttaacgtct atcagccttc cggtgagttg gaacagcatg ctcagcgttt
     4501 gcaagatatt ctccacggtc tgtagcagcg taggtttcaa tttaaatatc cccagatagc
     4561 cctctgggag cttccctttt tattaagttt taattaccgt tgcaatagtt aaatgatatt
     4621 ggtaattatt atcattagca tttgtgtctg tgttgtttct ggtaaggctg aaagggaaca
     4681 aacatcgcct gtacaacggc gtttttactt acagaccagc gaaggcgaga ctatcaggat
     4741 gatgcatcgt gtgtctaaga aatcacggtt ctttccagat tcgttgttcg gtctaacact
     4801 gcacaaatgc gtcagaacat cccggcgtag ctgtccgacg ccatttgggc tgttcaacag
     4861 gacaatggcg cgtaagcgtt aatggaagtg gggatacgga gttccctgtt cacctgtgta
     4921 tgtcacggaa aagtctctga ctgagctgtc cataacgctg gtgaagcaca aatggcgcag
     4981 catctggtaa agcaggttta acgaaaagtc gtgtcctgct gctggcatat gagtgcgtcg
     5041 actgcctgat ttagatcgtc aagcggagag ggattttctc atgcgtattt tgtttgtagg
     5101 cccgcctctc tatgggctgt tgtatcctgt gctgtctctg gcgcaggcat ttcgcgttaa
     5161 cggtcatgaa gtgctgatcg ccagtggagg acaattcgca caaaaagcgg cagaggccgg
     5221 gttggtggtg tttgacgccg cacccggtct ggattccgaa gctggttacc gccaccatga
     5281 ggcacagcgg aaaaaaagta atatcggaac ccaaatgggc aacttctcat tcttcagcga
     5341 agaaatggcc gaccatctgg tggagtttgc cggacactgg cggcctgacc tcatcatcta
     5401 ccctccgctc ggagtcatcg ggccgctgat tgccgccaaa tatgatatac cggtcgtgat
     5461 gcaaaccgta ggcttcggcc ataccccctg gcatatcaag ggtgtaacgc gttcgctcac
     5521 ggacgcctat cgtcgccaca atgtgggggc cacaccacgt gatatggcct ggatcgacgt
     5581 aacaccgccg agtatgagca ttctggaaaa cgacggtgaa cccattattc cgatgcagta
     5641 cgtcccgtat aacggtggcg cggtgtggga accatggtgg gagagaaggc ccgagcgtaa
     5701 acgtctgctg gtgagtctcg gcactgtcaa accgatggtt gacggccttg atctaatcgc
     5761 ctgggttatg gattctgcca gtgaggtcga tgccgagatc atcctgcata tttcagccaa
     5821 tgcgcggtcc gatttgcgct cgctgccgtc aaatgttcgc ctggttgact ggatacccat
     5881 gggtgtcttc ctcaacggcg cagatggttt cattcatcat ggcggagcag gcaataccct
     5941 gacagcgcta cacgctggta ttccacaaat cgtcttcggc cagggagccg atcgcccggt
     6001 aaatgcccgc gttgttgccg aacgcggctg cgggattatc cctggcgacg tcggcctgtc
     6061 gagtaacatg atcaatgctt tcctcaacaa tcgctcactt cgcaaagcct ctgaagaggt
     6121 agcagcggaa atggcggcgc aaccctgccc aggtgaagtg gcgaaaagcc tgatcacaat
     6181 ggtacagaaa gggtaaccac tacaccttca tgataatcat atgccagcac ccatccagga
     6241 gtcatgcatc cataacaatg cgggaccgga gcgtgctggc ttttttgcgg aacgatccgg
     6301 ccagtggatc gtattttaca gacggaggag gcttaatgcc tgcgaatcac actcccacac
     6361 cggctcagtc atggatagtt cgcctagcgc gcgtgtgctg ggaacgtaag aaacttagtg
     6421 tcatcgtggt ggtagcgtca gtatcgacta ttttgctggc tgcgctgacg ccactgctga
     6481 caagacaggc tgtgaatgac gcactggcgg gcaatccggc ccgcctgccg tggctggcct
     6541 gcgggttact gttgatcgct ttttttgatt tcatcggtaa ctatgtgcgc cgtggttatg
     6601 ccgggatgct ctcactctgg gtgcagcata ccctcagagg acgggtattc gacagtattc
     6661 agaaacttga cggcgcaggc caggatgcgc tgcgcaccgg gcaggtgatt tcacggacca
     6721 acagcgatct gcagcaggtg cataccctgc tgcagatgtg cccggtgccg ctggcagtgt
     6781 tcacttatta cattgccggc attgccgtga tgctgtggat gtcccctgcc atgacgctta
     6841 tcgtcgtgtg cgtactggta tgcctggcga tcaccgcgct tcgtgcgcgt cgtagggtct
     6901 tcgcgcaaac cgggctggcc tcggaccaac tggcgaatct caccgaacat atacgcgagg
     6961 tgctggcaca gatctcagtg gtaaaatcct gtgtggcaga gatgcgtgaa acgcactggc
     7021 tcgataggca gtcgcggcag attgtgcgtg tacgcatcgg tgcggttatc tcgcaggcga
     7081 tgcctggggc caccatgctg gcgctaccgg tgctcgggca aatcgtcctg ctgtgctacg
     7141 gcgggtggtc ggtcatgcac gggcggatcg atctcggtac cttcgttgca ttcgccagct
     7201 tcctcgcgat gctgaccggg ccaacccgcg tactggcatc gtttctggtt atcgcacagc
     7261 gcactcaggc gtccgtggag cgggtgtttg cactgatcga cacccgttca cagatggagg
     7321 acgggacgga gtcgattaac agtcaggttg tcggactgga actggagaat atgagctttg
     7381 actaccacca tggcgacaga catatcctca gcaatatctc cttttccctg cgcgccggtg
     7441 aaaccgtggc ggtggtgggc gcatcgggtt caggaaaatc gaccctgttg atgctactgg
     7501 cgcgttttta tgatccctgc tccggaaaga tatggctcaa caccagcgaa ggccgacaaa
     7561 atcttcgcga tatcagactg gaggcgcttc gtcgccgggt aggcatcgta tttgaagatg
     7621 cttttctgtt tgccggtacg gtggcggaaa atatcgccta tggccaccct caggcaacgg
     7681 cggacgacat tcgccgtgcg gcagctgctg caggagccag cgattttatt aacgccctgc
     7741 cgaaaggctt cgatagcctg ttaaccgaac ggggtacgaa tctttccggc gggcagaggc
     7801 agcgaatagc gctggcgcgg gcgctcatta ctgcaccgga cgtgttaatc ctggatgata
     7861 ctacctcagc ggttgatgct gttacggaag cggagattaa taccgcgctg ggtcgctatg
     7921 ctgacgaagg gcatatgctg ctggtgattg cccgacggcg ttcaacactt cagctagcca
     7981 gccgggttgt ggtgctggat aagggccgta tggtggatac cggaaccccg gcagaacttg
     8041 aagcgcgctg tccggcgttc cgcgcactga tgaccggcga cagcgatttt ctggccacgt
     8101 cccacaatag ccacaacgaa ttgtggccgg ctgaaccagc gacacaagac gatgtaacgg
     8161 atacggggga taaaggtttt gtcgcccgta tgacccgcgt accggaaaat gcagtacagc
     8221 aggcgctggc cggtaaaggt cgcaaagtca cgtcactact gaagcctgtg gcgtggatgt
     8281 tcgtcatcgc cgctctgctg atcgcactcg attctgcggc aggcgtaggg gtactgatac
     8341 tgttgcagca cggcattgac tccggtgtcg ccgcaggcga tatgtcgatc atcggcctct
     8401 gtgccctgct cgccctgtgc ctggtcattg tgggctggtg cagttattct ctgcagacgg
     8461 tcttcgccgc cagagcggcg gaatcagttc agcattcggt gcgcttgcgc agcttcggcc
     8521 atatgctgcg tcttggactc ccctggcatg aaaagcatgc cgattcgcgt cttacccgca
     8581 tgaccgttga tgtggactct ctcgcccgct ttctgcaaaa cggccttgcc ggtgcggcca
     8641 ccagtctggt gacgatgttc gcaatcgccg ccaccatgtt ctggctcgac ccgttcctgg
     8701 cgctgacggc attaagcgca gtgccagtgg ccgcactggc aaccatgatt tatcgccgcc
     8761 tcagtacccc tgcttatgca caggcacggc tggaaatagg caaagtcaac agcaccctgc
     8821 aggaaaaagt ctctggcctg cgtgtcgtgc aatcgcatgg tcagcaggaa ctggagggcg
     8881 cccggctgcg cgcgttatcg gagcgtttcc gcgcaacccg tgtgcgagca caaaaatacc
     8941 ttgcagtcta ttttccgttc ctgacattct gcaccgaggc ctcctatgcc gctgttctgt
     9001 tagtgggagc ttcgcaggtc gccgctggag aaatgactgc cggggtactg gcggctttct
     9061 tcctgttgct ggggcaattc tatgggccag tgcagcagtt atcagggatt gtcgacgcct
     9121 ggcagcaggc gacagccagc ggcaaacata ttgatgaact actggcgaca gaaggcactg
     9181 agaacctcgg gtcctcttcg gtcctccctg tcaccggtgc actgcatctt gatgaggtca
     9241 cgttcagtta tcccgacagt cacgagccag ctctgaacaa acttaccctg acgatcccag
     9301 agggaatggt tgtcgcggtc gtcggtcgca gcggtgcggg taagtcgacg ctgattaagc
     9361 tgattgccgg gttgtatttc cccacgcacg gcaacatcag aatcggtgtg caaatgctcg
     9421 atgatgcctc gctcactgag tatcgtcgcc agattgggct tgtcgatcag gatgtagcac
     9481 tgtttagtag tgatattgca gaaaacattc gttattcacg gccatccgcc accaatgaag
     9541 acgttgaaat tgcctcacag cgggcagggc tgtatgagat ggtgtgcaat ctgccgcagg
     9601 gattccggac accggtgaat aacggcggag ccgatctgcc cgcaggtcag cgccagttga
     9661 ttgcgctggc ccgcgcgcaa ctggcgaatg cccacatcct gctgctcgac gaagccacgt
     9721 catgtctgga tcgcacatcc gaagaacgac tgatgtcatc gttaacagat gtcgtgcatg
     9781 ccgggaagca ctcggcgctg attgttgcac atcgtctgac caccgcgcaa cgctgcgatc
     9841 tgattgccgt tattgataag gggttacttg cggaatacgg aacccacgaa cagctgttat
     9901 ctgcgggcgg cctctatacc cgcttatggc atgacagcgt cagcagtact gctctccatc
     9961 gccagcacaa catgaaggag gaaaccccgg gatagttact ggacacgtaa tgtattaaaa
    10021 acacagtcag aagcggcggt accgtgaata gccgctttaa ttatttatac tgacatcctt
    10081 aatttttaaa gagtatgaat gctgaacatg caacaacatc tctctgctat cgccagcctg
    10141 cgcaaccaac tggcagcggg ccacattgct aaccttactg acttctggcg cgaagctgag
    10201 tcgctgaatg ttcctcttgt gacgccagtc gaaggagcgg aagatgagcg agaagtgacc
    10261 tttctgtggc gcgcccgaca tcctctgcag ggcgtttatc tgcgtctgaa ccgggtgacg
    10321 gataaagagc acgtagaaaa aggaatgatg agcgcccttc ccgaaacgga tatctggaca
    10381 ctgacactgc gtttacccgc aagttactgc ggctcctatt cgctgctgga aatccccccc
    10441 ggcactacgg ctgagacgat tgcactgtcc ggaggccgtt ttgccaccct tgccggaaag
    10501 gccgatccgc taaacaaaat gccggagatc aacgttcggg gaaacgcaaa ggaatcagtg
    10561 ctgacacttg ataaagctcc cgccctgtcg gaatggaacg gcggcttcca caccggacaa
    10621 ctgcttacct ccatgcgcat tatcgccggg aaatctcgcc aggttcggct ctatattccg
    10681 gatgttgata tttctcagcc cctcgggctg gtcgtgctgc ccgatggtga aacctggttt
    10741 gatcaccttg gcgtatgcgc ggcaattgac gccgccataa ataatgggcg catcgtgccc
    10801 gtggctgtac tgggcattga caacattaat gaacatgaac gcactgagat actcggcggg
    10861 cgcagcaaac tgataaagga tatcgccgga catctgctgc cgatgattcg cgctgaacaa
    10921 ccgcagcgtc agtgggcaga ccgttcgcgc acagtgctgg ccgggcagag cctcggcggg
    10981 atcagtgcgc taatgggggc tcgttacgca ccggaaacgt tcggtctggt gctcagccac
    11041 tctccttcaa tgtggtggac gccagaaaga accagtcgac caggcttgtt cagcgaaacc
    11101 gatacctcat gggtgagtga gcatctgctt tctgccccac cgcagggcgt acgtatcagc
    11161 ctgtgcgtgg gatcgctgga aggttcgaca gtgcctcacg ttcagcagct tcaccagcgg
    11221 ctgattaccg ctggcgtcga aagccattgc gcaatctaca ccggtggtca cgattacgca
    11281 tggtggcgcg gtgcactgat tgacgggatt ggtttactac agggttgagt tgacccacaa
    11341 acactttcag gaaacggtac agacttcctg aataaatcaa atagtcacct gcggaaaagg
    11401 aataatcatc agatgtatgc ccgcgagtat cgctcaacac gcccgcataa agcgattttc
    11461 tttcatcttt cttgcctcac ccttatctgt agtgcgcaag tttatgcgaa gccggatatg
    11521 cggccactgg ggccgaatat agccgataaa ggctccgtgt tttaccattt cagcgtcacc
    11581 tctttcgact ctgtcgatgg cacacgccat tatcgggtat ggacggccgt gccgaataca
    11641 accgcaccgg catcgggtta cccgatttta tatatgcttg acggtaacgc agttatggat
    11701 cgcctggatg acgaactgct caaacaattg tcagaaaaaa caccgccagt gatcgtggct
    11761 gtcgggtatc agaccaacct ccctttcgat ctcaacagca gggcttacga ctatacgcca
    11821 gcagcagaaa gcagaaaaac agatctccac tcagggcgtt ttagccgtaa gagtggtggc
    11881 agcaacaact tccgccagtt actggaaacg cgtattgccc caaaagtgga acagggactg
    11941 aatatcgatc ggcaacgccg cggcttatgg gggcactcct acggcggcct cttcgtgctg
    12001 gattcctggc tgtcctcctc ttacttccgg tcgtactaca gcgccagccc gtcgttgggc
    12061 agaggttatg atgctttgct aagccgcgtt acggcggttg agcctctgca attctgcgcc
    12121 aaacacctgg cgataatgga aggctcggcg acacagggtg ataaccggga aacgcatgct
    12181 gtcggggtgc tgtcgaaaat tcataccacc ctcactatac tgaaagataa aggcgtcaat
    12241 gccgtatttt gggatttccc caacctagga cacgggccga tgttcaatgc ctcctttcgc
    12301 caggcactgt tagatatcag tggtgaaaac gcaaattaca cagcaggttg tcatgagtta
    12361 agccactaaa cactgcccgc ttttacgcgg gcagtacgcc tgaaacacta cgatcagaat
    12421 gatgcggtaa ctccggcata gtaagcccgg cctggctcgt tataggtatt cgccccttca
    12481 gaagatcgga agatctgttt attgaggata ttactgacgc cgacattaag acgcagattt
    12541 ttattaatat cgtaattgaa gttcgtcccc accagtgaat aagcgcccag ctctttacct
    12601 gacagaccgc cagtatcttc actgcgggtt tccgcatgag tacgcggttt ttgtctgcca
    12661 tataacgtcc agttgacgct ggcagaaaac gcctgggtga tggtccagtt aagcgagtta
    12721 ttgatagtat atttcgggat gaccgacaga ggattaccgg tgtctttttg ctccgaagtg
    12781 atcatccatg tggcattggt attccagttc agacgatctt tcaccagtgg gaaagacata
    12841 ctggcttcga taccgtccac cagagctttc ccgccattct gccacttgag gatatatgcg
    12901 cctgaagcgg tttgcccgat aacgttatcc ccggccacga tcttattctg gtaatcattg
    12961 cggaagtagg tcacacttgc gtggtaatct tcccaggtga actccagccc aatttcttta
    13021 ttgacgctga tttccggatc gagatcttta ttaccgatca ggtagcaccc gcctgatgta
    13081 atatcttttg gacagccatt gcctttcgag tagagcagat agccttcact ggattgatac
    13141 aggtttgggg ctttaaaggt tcgggcaacc cctgctttga ctttgaaata atcgcccaat
    13201 tcctgcgaaa gattcagact ggggctgaag ttcccgccgg agtcgctgag ataatcaaag
    13261 cgcaggccgg gaatgatatt cgtgccagga accggctcaa tgttatcttc aatatacagc
    13321 gcactgattt gagaatgatt tttactgctg cgatccgcag cagagccaga aataccgctg
    13381 atatcactgt cattcaccgt caggctggta gaggaaggat catcgagctt atcgcggttc
    13441 cactctgcac caacggtcag cgtttgatca accatcacat tcaaaggaat attaagctcg
    13501 ccgctggttc gccaggaact caggcgattg gtcgtaaact tttcacccgc taaaatacgc
    13561 ccttcaccac cgccggataa tccttcattc atgcgggtat tattggtttt ctcgtaataa
    13621 acaccaaagc gactttgtcc ccagtcccag ataccattat gcgtaatgcc ataattctgt
    13681 cggtacaggc ggttcgtctc tttgccggat tttgccaggc tttcggtaac tgcactggaa
    13741 gaactgtttt gcgtatcgcc cgcatagata ttcccctggc ggctatatcc ggcttcgaaa
    13801 tcgagaatct gctgcggatt taatttccac gagacaacgc cgttaatatc tttgttacgt
    13861 accccttcat gcccggctgc gtttttcgta ccgaccggag aattaatatc ccaactgtca
    13921 gcatccgttt tattcaggtt accatacaaa cgcgtggtaa gagcattacc agccagaggc
    13981 ccactaaggc tgaaattggc gcgacgcgta gcgccctcat cgctactttc cggctgattg
    14041 gtgtataacg acagcgaacc gtgccagtcg ttggtgggac gtttggtaat gatgttcacc
    14101 acccccccgg ctgcccccga accgtagcgc gccgccgcag ggccgcggat cacttcaata
    14161 cgctcaacct gttccggtgg cacccagttg gtgtcaccgc gggtatcacg ctctccacgc
    14221 cagctataac gcacggagtt acgtgacgtc accggtacac catcaattaa aattaaggtg
    14281 ttttccggcc ccataccacg aatatcgatc tggcggttgt taccgcgtgt gcccgaggcg
    14341 ctattgccgg taagattgac gccaggcatt ttacgaataa tatctgaaag gtcgtttacc
    14401 ggaggggtct ttttaatatc ctcgctggta ataaccgaca cgcccggctg ctgttttaat
    14461 acctgctcag cggtggcttc caccaccaga gtctcgtcat tatcatcgtc ggaggatttg
    14521 gctactgata cctggctatt caacccaacc aggagcacag ttagcgacca gaggattttg
    14581 ttaattctca tacctattcc ctaataaatg cctaacttaa aatgtttgat cgttaagctc
    14641 acatccttgc cagatatttt ttactgccat tattgttttt atataagaat gataattaat
    14701 atcatttagc aaaaaaaaaa gcaatccctc acaagataaa tatatcgatt tttcataaat
    14761 atcaaattga tatataacat atgtttttta tttcattgca cttgtcgatc atcgggaatt
    14821 ttgtcacaat ttctcacgga tagtgttcac attgtttctg acctgcattt caggcgcggg
    14881 cgctgcttat gtatataagg gcctgtggtc gacgaatcag cataatcaga tcttcctgcg
    14941 agcaatttct ttcagcgtat caggcagcac tatcggatta tctacgtgcg tttttgattc
    15001 acattgtgtg tgaaacttca tacatcatga ttcgctttcc ctactcagtt ctgatgattg
    15061 ttttttattc tattaacacg gagttaagat tctaccctcg gtgtcctcat tattaatgag
    15121 gaatgctacc tatctaaaaa ctgaccgaaa ctttggggca tccctggagg tttccaagga
    15181 gaaaaaggtg acagtgatag tgccacattg tgaggaaatt cctgagaaac tcctggttca
    15241 cacacataat tcaggagttt gccatagata gtaaccaatt gttttatatt ttaagaccat
    15301 taaatacgcg gctacgatag cgctttatat aatcataaac tgtagagata aaatatctgc
    15361 ttctctgata acactaattg agtgtcttaa actaagtaat ttcatttttc cccctttaaa
    15421 agcaatatat gtgcaatagt tgaagttaaa gctgtgctgc cagagttgat aataattgtt
    15481 ctgtctgttg ccatgatatg cattcatcag taacagactg accatactct agcggaaaac
    15541 tatcggattg ttttccatct tgtaaaaagc tttccaccat aatgccagct acatatcctg
    15601 gtattttttt ccgattatct ataacttgac gtgcaacgga aatttgccgt ttagccactt
    15661 taccgctatt accatggcta caatcaatta tcagacgatg gttaataccc tcatcatgca
    15721 ttaatttcac tgccttagtt atatcagaca aaccatagtt aggttctcta cctccacgta
    15781 atattagatg tccatgtgga ttgccatcgg ttaatagtgt agatatacta tttgttaaag
    15841 atgtcatata tacaatatgt tgttcacggg ctgcaataat agcgtcaata gctaagttaa
    15901 tatttccatc agtactgttt ttaaatccaa ctggacaatg taaaccagac gcaagttgac
    15961 gatgcgtttg tgattcagtg gttctggcac caatagcacc ccaacatatt aaatcagcaa
    16021 tatagggagt aagaaaagga tcgaggaatt ctgtagcggt tgcgacacgc atcgttgtga
    16081 ttgaagacaa acactggcga gcatatcgta tccctttctc aacatcataa cttccattta
    16141 gatcaggatc gtgcattatt cccttccagc ctttacgggt tcgaggtttc tcaaaataag
    16201 tgcgcattac aatgtacatt ttcgataagt acttattctg taatacgaac aatcgcttcg
    16261 cataatcaac tgcggcctga acgtcatgga ttgagcatgg accaacaatc acaagtaagc
    16321 gaggatcttt tcccagcaaa atattcgcta taatatctcg ttgcagagag atccatgtta
    16381 cggtttcttc cgatactgcg atttctttat gaatctcgcc cacagtgggt aaactgccaa
    16441 gcaacttccc gcggtagatt aatttactag ctgactgcat cttgtctcct aaaagcgcta
    16501 tttcagacca tcagttgcac acagtagatg ataatgataa tcatttactg tataattggc
    16561 aatatatggg gattcatagt catttctgag ataatatacg ttaggccttc tctcccgtac
    16621 ggaaatcact tgttttccgt aaattacctg aatcttaatc cctgagctgc ggctagctgt
    16681 ggaactcaca aaagctgacc gggccgtggc acagcctctc tgctggcaga acagcgtact
    16741 gcagaagtcg tcgtactgcc gctggtcccg gcatcaggtc tgcccgcaga tggcacttct
    16801 gccactgatc tgccggtgga gctgaacggc atgacatatc aggcctgccg tggggatttt
    16861 gtggtgcgcc tcgacggcag cacctgtctg cagttataga ataaagaagg cggggaggta
    16921 cgcagggagg gcgatccgct ggaagtggcg tagtggctgt aggcctgtaa tgatgcagga
    16981 atagaagttc gtgtacaggt taatgaaagc atcaccccgt aaaaacatgc cggcacggtt
    17041 tacacatcat tccatggtgg cagccggtta actttcctgc gccaccagtg gaaagaggcc
    17101 atgagcagtc cgaacaaaaa accactcgtg atacttataa taatggcctc acctgctacc
    17161 attcctgacg gcccccagta cataaaccac attgcacatc cccaggaaat accccataag
    17221 ccccccatca gcagcgtaac ctgccagaac ggcataaagg gcaatggcgg aagccggata
    17281 cccagccgcc agagaatacg cagcagagga ggagcataat tactccgcca catctttttg
    17341 ctgtccatca gggcaattgc ccgggctttt tttgctcaaa agtcacagga gcacctccgt
    17401 tccatgatgt aaacgtgaca caacttattg ttattgatta ttattatcat accgtgatta
    17461 tgttgtcatg cacctgtaca ctgataaaag aaagggagaa caggaggctg gctgaacaac
    17521 caatccgttc gtccagttca atggcggcta tcaggttctg ctccgcaatg tactcacgaa
    17581 tgcgcttcct gtcctggctg gtcagcatgg tccagaatat ctccatcatt taccggcgac
    17641 tttcctgctc atttcatccc gccatgcagc agcggaagat tcgaccatgt catggggaag
    17701 cacatcgccg cgctcgagtt gctgacgtcc tgcctcaacc tggtcacgga accaggtatt
    17761 atgctgccgt tccacggtct ggcgcataaa atcccggatt aactgagagc cattacggtc
    17821 catgcttttt gcagggccat aaaagcatcc ttcagttcag cgtcaattct taaactcata
    17881 ttaacctgtg ccatttatca ccccgtcatt accacgtata tacacagtat ataacgattc
    17941 agatatcgtc actaggatat gccgcgccag cggcatggaa ggcggcactc cgctgtttca
    18001 tatgataccg ccgaagcccg atgtaagccg ctacagtcgt ccgaaagtca ccagcctcct
    18061 cccccctgcc gtcatccgtg catcagctat gcactgagta tgcctgccct tccctagaga
    18121 atcctgctag gcttgccaca ctgatatatc ttgactttat gtaaacgata tgacacttta
    18181 acatgataat gattaccatt ctcttttaat atacagagaa actaggaaat agatgaatga
    18241 gttatgttac tttaa
//
LOCUS       DAZFZC010000095        18073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFZC010000095 DAZFZC010000000
VERSION     DAZFZC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.096"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..566
                     /locus_tag="ACHKXP_003853"
     CDS             <1..566
                     /locus_tag="ACHKXP_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO7648771.1"
                     /translation="PAGWAVNTLSQIANITMGQSPAGESYNEDGIGTLFFQGSTDFGW
                     LFPTPRQYTTSPTRMAKKGDILLSVRAPVGDMNIANADCCIGRGLAALNSKSRSDGFL
                     FYVMKYFKQVFERRNAEGTTFGSMTKDDLHSLQVVCPEPGLLKRYDDIVSEYNKMIFT
                     RSLENQDLIKLRDWLLPILMNGQVKIK"
     gene            complement(807..908)
                     /locus_tag="ACHKXP_003854"
                     /pseudo
     CDS             complement(807..908)
                     /locus_tag="ACHKXP_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1272..1403)
                     /locus_tag="ACHKXP_003855"
     CDS             complement(1272..1403)
                     /locus_tag="ACHKXP_003855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648772.1"
                     /translation="MTEQKITDIAQAKTNFYLFSINARGNHAGKIKLSHNQLLNWLV"
     gene            1628..2548
                     /locus_tag="ACHKXP_003856"
     CDS             1628..2548
                     /locus_tag="ACHKXP_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO7648773.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDGLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IVQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            2716..4233
                     /locus_tag="ACHKXP_003857"
     CDS             2716..4233
                     /locus_tag="ACHKXP_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO7648774.1"
                     /translation="MYKKTTLAVLVALLTDATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            4294..5664
                     /locus_tag="ACHKXP_003858"
     CDS             4294..5664
                     /locus_tag="ACHKXP_003858"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFO7648775.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADIARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNTDNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLFK
                     YKTDEE"
     gene            5664..7073
                     /locus_tag="ACHKXP_003859"
     CDS             5664..7073
                     /locus_tag="ACHKXP_003859"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO7648776.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVISLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLNTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFMHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGGPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            7099..8106
                     /locus_tag="ACHKXP_003860"
     CDS             7099..8106
                     /locus_tag="ACHKXP_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7648777.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSTIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(8658..9131)
                     /locus_tag="ACHKXP_003861"
     CDS             complement(8658..9131)
                     /locus_tag="ACHKXP_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO7648778.1"
                     /translation="MCKLTKEEEYSIISRTMTNIRSLRTYVRELDFEQLLDMQEKLNT
                     VIEERREDAERKAAERKELETKCQQVFEYIVSVGLDPEEWQGPVSAIAGTTETKRKPK
                     GGVRKAKYIFEDENGETRTWSGNGKMPLALRKQVNGDCTLETFLIENPNQHEQSE"
     gene            9450..10187
                     /locus_tag="ACHKXP_003862"
     CDS             9450..10187
                     /locus_tag="ACHKXP_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO7648779.1"
                     /translation="MKKVIAVSALAMAGMFSAQTLADESKTGFYVTGKAGASVMSLSE
                     QRFVDGEGAWADKYKGGDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESVGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGVGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
     gene            10607..11503
                     /locus_tag="ACHKXP_003863"
     CDS             10607..11503
                     /locus_tag="ACHKXP_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7648780.1"
                     /translation="MADSTRNRSAERLIDILVELQNYGVVSRHNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKESLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAICNSNVCQIAYAGKIRIVHPYKLTN
                     QRSIWYLLATEENKLKSFSLAKIKWLDIKKEKFAKSDEIQSLVSESCDPWVSDKTFDV
                     VLFIHSNIAHYFLRRDLLPYQQLLHKHDNGITLSCKASHKNQIIPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            11552..12631
                     /locus_tag="ACHKXP_003864"
     CDS             11552..12631
                     /locus_tag="ACHKXP_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HFO7648781.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDIFEKAKERYE
                     EHKGKFEQAEALTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNLNKIHNLAISATEYISTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMATIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACDVERAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DISVSDEVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMETDDNGMN
                     VINKEELDDVLASSVVKFDDFSSRS"
     gene            12678..14249
                     /locus_tag="ACHKXP_003865"
     CDS             12678..14249
                     /locus_tag="ACHKXP_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HFO7648782.1"
                     /translation="MNVEKFDWTEELHQTMVKSLVTSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGDYDSHHYHSHENYIATNRKGKRAHQAGTLTDAYTGEVFA
                     SDDNKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQLAELESQVTLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFCAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAALNHFSLHLDSVNSEIERGLLLREEVSRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            14652..14849
                     /locus_tag="ACHKXP_003866"
     CDS             14652..14849
                     /locus_tag="ACHKXP_003866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648783.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVGLGML
                     FLMIMIAGGMCGSLLSRKKTS"
     gene            14915..16588
                     /locus_tag="ACHKXP_003867"
     CDS             14915..16588
                     /locus_tag="ACHKXP_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7648784.1"
                     /translation="MYLPVTACSASGAGQKYRFYSIIMKINLPFIHWPRISNDKRLVL
                     SVVVFTLSNLLVSSGAHLIYRILFFILALLIIKAMKSITLRLIIAFPFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPSEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEYQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARTDNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSADSVLHHDIRNYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGSRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFAMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPTLGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNELQE"
     gene            complement(16732..17775)
                     /locus_tag="ACHKXP_003868"
     CDS             complement(16732..17775)
                     /locus_tag="ACHKXP_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7648785.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHKQAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVRHNFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPF
                     SAAGMKHSSIQHQ"
BASE COUNT         4833 a         3799 c         4315 g         5126 t
ORIGIN      
        1 ttccggcggg gtgggcggtt aacacgctgt ctcaaatagc gaatatcaca atggggcagt
       61 ctccagccgg ggagtcttat aatgaagatg gaatcggaac actatttttt cagggttcga
      121 cggattttgg ttggcttttc cctactcctc gccagtacac aacatcacct acgagaatgg
      181 cgaaaaaagg tgatatttta ctcagtgttc gtgcgcctgt tggggatatg aatattgcca
      241 atgccgattg ttgtattggg cgagggcttg cggcattaaa tagcaaaagc cgctcagatg
      301 gatttttatt ctacgtgatg aagtatttca aacaggtatt tgagcgtcga aatgcggaag
      361 gaacaacatt tggctccatg acgaaagatg atttacattc actgcaagtc gtttgcccag
      421 aacccggatt actgaaacga tatgacgata ttgtttcaga atacaataaa atgattttta
      481 cgcgtagcct tgaaaatcag gatctaatca aactgcgtga ctggctcctc cctattctta
      541 tgaacggaca ggtcaagatt aaataacccc caacgtattt ttcgaagcgc agtccaagtt
      601 tgaactgcgc ttcacttccc ccaacaaaaa acctgccatc cattactggt agtgattcct
      661 ctcacatttt ccatgtatta attattattg ttactactac acccaaacga cctataatcg
      721 taagcggctc gccagaaccg tattgatatt tactgagcgc tcagatcaac tttccagggt
      781 aacagatcgc gtatccagtt tgcctgtcat tcctagatat gttcaatgac gtaacgcagc
      841 catttttctg gagccatatt gttcagacgg catgtaccaa tccgcaggta cagcatcgcc
      901 gcatcctcaa gtaatgccgg taatttgggg cgtaattctg ttgcgctttt actgaacaca
      961 atgccgtatt cagtaagaag tccaagaaca tgatctatca gttgtgtcct tccatcgaga
     1021 tcaaccgatg cattttcaaa aaagcctgga cgttctgtag gtccagactt tttattggta
     1081 ccagtcgtat agttctatat tgataggttt cagctattgc tgtgcgtcgt tataatcatt
     1141 tttctgacgc cattgataag atttaacatg ttgtgctgga tgcaatatta catcatagtt
     1201 gagttccctt atttcctgtg ggcagtgatg ggatgtcccg caagccttca tcgcaacggt
     1261 cattttggct gttataccag ccagttaagc agttggttgt gagaaagttt gatctttccc
     1321 gcgtgattgc cacgagcatt gatactgaaa agataaaagt tcgttttagc ctgagcgatg
     1381 tcggtaattt tctgttcagt catggccttt ctcttacttt tgtggttaaa atttattcgc
     1441 tatggcgcaa ttgaaagcgg tcgtgaaggt gggggaagac tatctcatca cttaccatat
     1501 ttccgacata ttctcacaac aaaataccac gtaactattg atgaatattg ttctctcctg
     1561 agcagcgttt atgctttaat tgctaaatcg atttaattag tttagcaatt agtatattgg
     1621 agcctttatg agcacaagag tatgggtact tggtgatgcg gttgttgatt tattacccga
     1681 aagccagggg agactactac agtgtcctgg cggggcgcct gctaatgttg cagtcggtat
     1741 cgcaaggctg ggggggaaaa gtgcctttat tggcaaagtt ggcgatgatc ctttcggtcg
     1801 ctttatgtat cagacactga gtacagaaaa tgttgataca cgttatatgt ctcttgatcc
     1861 tcaacatcgc acctcaattg tggctgtagg acttgatgag caaggagaaa gaaactttac
     1921 ctttatggta cgcccaagtg ccgatctttt tttacaacct gatggccttc ctgcatttgg
     1981 gccgggtgaa tggctccatc tttgttccat tgcgctcagt gcagaacctt cccgaagtac
     2041 cgcatttctg gctatggaga aaatacgtca ggctggcgga aacatcagtt ttgatcccaa
     2101 tatccgcagc gatctctggc agagtgaagc gctattaagg aaataccttg atcgcgcact
     2161 ttcgctggcg aatatcgcta aattgtccga agaagagttg ctattcatca gtggcgaaag
     2221 ccaggttcag caaggcgcat attcattagt acaacgttat tcgttgactt tattgcttat
     2281 cacacaagga aaaaatggcg tacttgtgta ttttcagggg cagtttatcc actatcccgc
     2341 caaacctgtt tctgtcgtcg atacgaccgg ggcaggagat gcttttgtcg ctggattact
     2401 tgccggtctg gctgattctg gaataccaac aaataccaga cagcttgaac gaatcattgt
     2461 acaagctcag atttgtggtg ctctggcgac cacggctaaa ggcgcgatga ccgccttacc
     2521 ccgacaacac gatctccctt cacaatagtg atcctgctgt tgtatctgta acccgatttg
     2581 tcgtgcctgt cacatttact aaatcggttt agcgaaaata attgctttgg ctaaaaagtc
     2641 agctttatta tcaacaccta aaccggttta gcattttagc aatttttata attctctgat
     2701 gggacgcgac aaattatgta taaaaaaaca actttggcag tgttagttgc tttgctgacc
     2761 gatgctacaa cggtacatgc gcaaacggat attagcagta tcgaatctcg actggcggcg
     2821 ttggaacaac gtttaaaaaa tgcggaatcc cgcgcccagg cggcagaagc aagggccaaa
     2881 acagctgaat tacaggttca gaaactggct gaaacacaac aacaaaatca gctaacaact
     2941 caagaagtag cacagagaac agttcagctc gaacagaaat ccgcagaaaa cagtggtttt
     3001 gagtttcatg gctatgcccg ttccgggtta ctgatgaatg atgccgcttc cagtagcaaa
     3061 agtgggccgt atctgactcc cgcaggtgaa actggtggag ctgttggccg tctgggaaat
     3121 gaagccgata cctatgtcga gttaaatgta gaacataaac aaacactgga taacggtgcg
     3181 accacacgct ttaaagcaat gttggctgac ggacaaagag attacaacga ctggactggc
     3241 ggctccagta acctgaatat ccgacaggct tttgccgaac tgggcgcatt accaagtttt
     3301 accggagcat tcaaagacag tactgtctgg gctggtaaac gctttgatcg cgacaatttt
     3361 gatattcact ggttagactc cgatgtcgta tttttagcgg gaacgggcgg cggtatctat
     3421 gacgtaaaat ggaacgatac atttcgcagt aacttttctc tctacggacg taatttcggc
     3481 gatcttgatg atatcgacaa taacgttcag aattacatcc tcaccatgaa tcattatgca
     3541 ggccccttcc agttgatggt tagcggatta cgggcaaaag ataatgatga tcgaaaagat
     3601 gccaatggtg atctcattca aactgatgct gcaaatactg gcgtacatgc gttagttggt
     3661 ctgcacaatg acactttcta tggcctgcgt gaagggacgg caaaaacagc actgctatat
     3721 ggccatggcc tgggtgcgga agtcaaaggg attggctccg atggcgccct gctgtctgag
     3781 gcgaatactt ggcgcttcgc atcttacggc acaacacctc tgggaagcgg ttggtatgtc
     3841 gcaccagcaa ttctcgcaca aagcagtaaa gatcgttacg tcaaaggcga tagctacgaa
     3901 tgggtgacct tcaatacacg tctgatcaaa gaggtaacac agaattttgc tctggccttt
     3961 gagggtagct atcaatatat ggatctgaaa ccaaaggggt atcaaaacca caacgccgta
     4021 aacggcagct tctataaact cacctttgct ccaactctaa aagctaacga tatcaataat
     4081 ttctttagcc gtccggagct tcgcctgttt gccacctgga tggactggag cagcaaactt
     4141 gatgattttg ccagcaatga cgctttcggc agcagtggtt tcaatactgg cggagagtgg
     4201 aattttggtg tccaaatgga aacctggttt taataccaga cagcctcaca gatgtgaggt
     4261 ttttactgaa ataacaatga attagagggt tatatggatt tcgataaaat cgcccaatcg
     4321 ttgcttcctt tattaggtgg taaggagaac atcgccagtg cagcacattg cgccactcgc
     4381 ctacggctgg tactggttga cgacacactt gccgatcaac atgccattgg ccagattgat
     4441 ggagtgaaag gttgttttcg taattcaggg caaatgcaaa ttatctttgg caccggcgtg
     4501 gttaataaag tctatgctgc ctttattcag gttgcaggta ttagcgaatc cagtaaagca
     4561 gatatcgctc gactcgccgc tcaaaaactc aatccttttc agcgaatagc acggctactt
     4621 tctaatattt ttgtccccat cattccagcc attgttgctt ctggtttatt aatggggctt
     4681 ctgggaatgg tgaaaacata tggctgggtg aataccgata atgcgattta tatcctgctg
     4741 gatatgtgca gttcagccgc atttattatt ctccccatcc ttattggctt tactgctgct
     4801 cgtgaatttg gtggaaatcc ttatcttggc gcgacattag gagggattct gactcacccg
     4861 gcactcacaa atgcctgggg cgttgctgcg ggatttcaga caatgaactt cttcggtttt
     4921 gaaatcgcca tgattggcta tcagggaacg gttttccccg tgcttctggc agtatggttt
     4981 atgagcatag tggaaaaaca gttacgcagg tttatccctg atgctctgga tctcattctg
     5041 acgccatttc tgactgtcgt catttctggc tttatcgctc ttttgattat tggtccggca
     5101 ggacgagcgt taggtgacgg tatctctttc gttctcagca cgctgattgc acacgcaggc
     5161 tggttagcag ggttgttatt cggcgggcta tattcagcga ttgttattac gggtattcat
     5221 catagcttcc acgcaattga agcaggactt ttaggtaacc ctgcaatcgg tgttaatttc
     5281 ctgctgccta tttgggcaat ggcaaacgtt gcgcaaggcg gtgcatgtct ggcggtatgg
     5341 tttaaaacca aagatacaaa aattaaagca atcaccctac cctcggcctt ttccgcaatg
     5401 ttagggatta ctgaagccgc tatttttggt atcaaccttc gttttgttaa accttttatt
     5461 gcggccttaa ttggtggcgc tgccggcggt gcctgggttg tctctgttca cgtttatatg
     5521 actgccgttg gtctgaccgc aatacccggc atggcaatcg ttcagccaac atcgttggtt
     5581 aattatatta ttggaatggt gattgctttc gctgtcgctt ttagcctctc tttattattc
     5641 aaatacaaaa cggacgagga gtaatgtcga tgccttcaca aatgcccgcg attttacagg
     5701 cagtgatgaa aggtcagtcc aaagcactgg cagatgccca ctatccttgt tggcatctcg
     5761 ctcccgtcac cggactaatg aacgatccaa atggcttttg ctggtctgga ggccgctatc
     5821 atctgtttta tcagtggaat ccactggctt gtgatcataa gtacaaatgt tgggggcact
     5881 ggagttcgac ggatttattg cactggcagc atgaaccatt agcgctaatg cccgataaag
     5941 agtacgaccg taatggttgt tactcaggaa gcgcggttaa taatcagggc gttctcacac
     6001 tttgctatac cggtaatgtt aagtttgatg atggcagcag aacggcatgg caatgtctgg
     6061 ccaccgaaaa taaccagggc gggtttgata aactaggccc tgtcatatcg ttacctgatg
     6121 gttacaccgg acatgttcgt gatccaaaag tctggaagca caacagccaa tggtacatgg
     6181 tgcttggcgc gcaagataaa gagaaacggg ggaaagtgct gctgtattcc tctgtcgatc
     6241 tgaatacctg gtcttttcat ggagaaattg ctggtaacgg tctgaatgaa atagataacg
     6301 caggatacat gtgggaatgt cctgatcttt tcgcccttga tggtgaatac attctcctct
     6361 gttgccccca gggaatggtg cgcgaacatg agcgctatct gaatacttat ccctgcgcct
     6421 ggctacatgg acagtttgat tacgagacag gcaaatttat gcatggcgct ttctcagagc
     6481 tggatgcggg atttgaattc tatgcaccac agacgatgga agccccggac gggcgacgat
     6541 tgctggtcgg ctggatgggg ggtcctgatg gcgaagagat gttgcagcca accagaaagc
     6601 atcattggca gcatcaaatg acctgttttc gcgaactctc attccagaag ggaaaattgt
     6661 ttcagatgcc aatcagggaa ctcgcgcaac tgcgtgaagc tgaacacttc tggcaaggaa
     6721 aagcggatca tgcgccgcat gtcgaaattg aacgcctgga aatggacatc attccgtcgg
     6781 gtgagctgta tctgaatttt ggcaatgcgc tggcactgca tcttaatgac gatggtattc
     6841 aactacagag gagaagcctt gcagggcaag agaaactaac tcgttactgg cgaggaagcg
     6901 taacgtcact gaagattctc tgtgacagtt ccagcgtaga gatattcatc aataatggtg
     6961 aaggtgtaat gagtaatcgt tattttcccc atcatccagc ttcgctgata ttacagggtg
     7021 agtctgacgt cacgttacgc tactggtcgc tacgcgcctg catggtagaa tgagggtttt
     7081 gaattacgga tcctcgtcgt gagaaaaacc aaacgcgtta ctattaaaga cattgctgaa
     7141 ctggcgggag tgtcgaaagc aaccgccagt ctggttctca atggtcgtgg caaagagtta
     7201 cgtgttgcgc aggaaacgcg tgagcgtgtg ctgagtattg cccaacagca gcattatcag
     7261 cccagcattc atgcccgctc attacgggac aaccgcagtc ataccattgg tctcgtcgtt
     7321 cctgaaatca ctaactacgg atttgcagta ttttcccatg aactggaaac attgtgtcgg
     7381 gaagcgggcg tccaactcct catatcctgt actgatgaaa atccttcaca ggaaacaatg
     7441 gtggtgaaca atatgattgc ccgccaggtt gatgggttaa tcgtcgcatc cagtatgctg
     7501 catgataatg actatcaaaa acttagcgag caattacctg ttgtgttgtt cgatcgccac
     7561 atgaacggca gcacattgcc attggtcatt actgactctg tttctcccac cgccgctctg
     7621 gtggcagata ttgcacggag tcacccggat gaattctatt ttctgggggg acaaccccgt
     7681 ttgtcaccga ctcgcgatcg actggaagga tttacacaag gcttgcagca agccggagta
     7741 acgctacaac ctgaatggat tattcacggt aattatcatc cgagctcagg ctatgaaatg
     7801 tttgccgcgc tatgcgctcg tttaggacgt cctcctaaag ccttattcac tgccgcttgt
     7861 ggattactgg aaggcgttct gcgctatatg agccaatata atttactcga cagtacaatc
     7921 cacctggcga gttttgatga tcattatttg tatgattcac tttcggtaag aatcgacaca
     7981 atacaacagg ataatcgcca acttgctttt cactgctttg agcttatttc gcaactgatt
     8041 gaaggtgaaa cgccatcccc attacagcgt tatcttcctg ccagtttaca aaaacgctat
     8101 agataattta ttaattatct tatgtagctg agtattaggt gacaaatgat cagtctttcc
     8161 cacgacaatt gtatcgttct gatgaactca tattgacgca tttttcgggc gtatcagtcc
     8221 ccggtgttac gaatggtgca tacgaacgat cgcagtttga cgatggttga tttattttgt
     8281 tgcgcagggg caatcgcctg ctaggtaccg aaggctggct ctgaagtact gagtcgggtg
     8341 atcgcgtctg gccgctggct gcgaattact gacacgggat tggtggcagg tgatgaaatc
     8401 ctgtcccgga aatggctccg gattgattca agttctgacc cgctgtcagc ccgcgtggtt
     8461 gctgctctgg ctcatttcct gtcgtcagtt ttctcatgcc cggctgcggg ttttcggact
     8521 gaatgataat atctgacgac aggagtcgct catctatcga aggtaaatct ccctgaaagt
     8581 aatagttctg gcagggggaa cagaactgta atcattgtac cgtaatttga gttggctacc
     8641 tttgctacat gaaaacttta ttcactttgt tcatgttgat tggggttttc aatgaggaaa
     8701 gtttccaggg tacaatctcc attaacctgt ttccgcaaag caagtggcat tttaccgtta
     8761 ccagaccacg tcctggtttc gccgttctca tcctcaaaga tatatttcgc ttttctcaca
     8821 ccgcctttgg gtttgcgttt ggtttccgtt gtccctgcaa tagcagaaac tggaccctgc
     8881 cattcttccg ggtcaagccc cacagaaacg atgtactcaa atacctgttg acatttagtt
     8941 tccagttcct tacgttcagc cgcttttcgt tcagcatctt cacggcgttc ttcaataaca
     9001 gtgttgagct tttcctgcat gtcgagtaat tgctcaaaat caagttcgcg tacatacgtg
     9061 cgtagtgaac gaatattcgt cattgtccga ctgatgatgc tgtattcttc ttctttagtc
     9121 agtttgcaca taattcccct ctcttttgca ataaacgtgt tttctctggt gggttgatta
     9181 cccagtcttt tttactgtgt ctgacatttt gtactgtggt gcagttgtaa tgcaaacctt
     9241 ccgggctttt gggacacagc atttcagtcc gggccccatc attaccattt tgttgattcg
     9301 aaacgaaaac cctcaccaat atcgatcgat tttaccgatc gatatgatca catcgatcgg
     9361 ttgtaaccat tttaccctgt cttttgcatg gttttaaaat aggcaaccct ctttgtcggg
     9421 tagggcaaga accaacggag atgattgaaa tgaaaaaggt tattgcggtt tcagcgcttg
     9481 caatggcagg catgttttcg gcccagactc tggctgatga gagcaaaaca ggcttttatg
     9541 tgaccggtaa agcaggtgct tctgttatgt cactttcaga gcaacgtttc gttgatggtg
     9601 aaggggcatg ggcggataaa tataaaggcg gtgacaaaag tgatacggtt tttggcgcag
     9661 gccttgcggt gggatacgat ttttatcagc actacaatgt gccggtgcgt actgaagtgg
     9721 agttttatgg ccgcggtaac gctgaatcaa aatatcgtct gagttattgg gaaagtgtcg
     9781 gtggtgccga gtttgatgat gcacagaaca agctcagtgt aaacacgctg atgctgaatg
     9841 cgtattatga tttcagaaac agcagtgcat tcacgccatg gatatctgct ggtctgggtt
     9901 atgctcgggt tcatcataaa acttcgtaca tatataccga taacagtccg gcaggcagtg
     9961 aagtatattc agcttcagcg tcaaaatacg aaaataacct ggcatggagc cttggtgtcg
    10021 gtgttaaata cgatgtgacg caggatttca gccttgacct cagttaccgg tatctggatg
    10081 caggggattc cactctgact tataaagatg aggatggcgc taaatataaa tcctctgttg
    10141 atgtcagaag taatgagttt atgttaggtg caacatataa cttctgatgg ccatttctgc
    10201 ctgcgctttg gagcacagta aagtgcaggt atgacaaatc aataacagat atcctgatga
    10261 tattctccct ctggcaacta ataatttcag atgaatgaaa tataaataga gcaaaaatat
    10321 tactctcgtt gattttttac agggggcagg tagcgcgcag tatggttgtc agtctgttaa
    10381 atgaaaaagg actgttgttg ggacacgttg ttttttgtca tatcagccct tccggaaaca
    10441 ataaaccgta ttacgaagga ggctcccgga catctggttt aaagttcgaa cataatcaaa
    10501 taaaaatctg ttgccggagg gcgtagtttt gtggaactga caaatgatgt caggagtatt
    10561 tatataattc acttacacaa gagtaatact acaggtgcag tgatttatgg ctgactcaac
    10621 gagaaatcgc tcagcggagc gtttgattga tatccttgtt gaattacaga attatggtgt
    10681 ggtcagtcgc cataatttga tgaaaaaata caacatcact gagcgaacag catatcgtga
    10741 tctgaatatg ctttctccct ttattgaagc ctgtggcgat ggaaaatacc ggcttatttc
    10801 agcaagagca ggaaaccaga gcaaagaatc cctgcacaaa tctctggcca ggttactgga
    10861 tactgatgcg atttttcctg aacgggatga aggtttctgg cagaaattag aaaacagggc
    10921 aaccgaaaag catattcgtg ttcagtttca taaccctgag cacactatca gggatgattt
    10981 aagaaaatat cttgatgttc ttgaaaaagc gatctgcaac agtaatgttt gtcagattgc
    11041 atatgccggt aagatcagaa tcgttcatcc ctataaatta actaatcaga gaagtatatg
    11101 gtatttgctg gctactgaag agaacaagtt aaagtcgttt tcattagcta aaataaaatg
    11161 gctggatata aaaaaagaaa agtttgcaaa gagtgatgaa atacagtcac tagtaagtga
    11221 gtcatgtgac ccgtgggtga gcgacaaaac atttgatgtt gtgttgttca ttcacagtaa
    11281 tatcgcccat tattttttgc gacgggactt acttccttac cagcaattgc tacacaaaca
    11341 tgacaatggc attacgctgt catgcaaggc ttcacataaa aatcaaatta tcccgttgat
    11401 tttatactgg ctaccgaatg ttgaaattat tgaaccagta tggctgaaag aagccgtgtt
    11461 aacaatgtta ggaaaatacc tcaccgctga gaatgtttcc tgaaatattt cagttgactg
    11521 atgataaagc aaactgtaca ggaggaaaag aatggcgtta cctttcatta ttttgggtgt
    11581 ggctgccggc gctgcggcat tcggcggaaa aaaagcttac gatggatatc aaaaaaagtc
    11641 agaagcagag gatatcttcg aaaaagcaaa agagcgttac gaggagcata aaggaaaatt
    11701 tgagcaggct gaagctctga catcatcaaa attaacaacg ctcggaaata aagagcttga
    11761 gattggtaaa gatttcagtg agttcgacag gattgctgct gagctcctgg cgcgtctgga
    11821 aaaagaaggc aataaagacc tgaaactgag tgtcccgcaa cataacctga ataagattca
    11881 taacctggcc atttcagcaa cagaatatat cagtacggtc gttgctgccg gagtttccgg
    11941 ggctgctgcg ggttttgctg tgtatagcgg agtgatggca tttgctgcgg cttccacagg
    12001 tacacccatt gcagcgcttt ctggtgccgc ggcttataac gcgacgatgg cgacaattgg
    12061 tgggggctca ctggctgcag ggggcatggg aatggccggc ggcgcaatgg tattaggcac
    12121 cgcagtggct gcaccgttga ttgccattgc gggctgggct tatgacagac acgcgacgaa
    12181 agctcttgaa aatgcccatc agtgtgcctg cgatgttgaa cgggcagtta agaaaatgaa
    12241 tctggcccgg gagcattttg caaaagttaa tcaatacgtt gatgaaattc tcgcagcact
    12301 gaacagaatg catgctgtat ttattaaaca ctattttgag ccgcttaaat ccatgcattc
    12361 tgttattaca gaacaacagg atatcagtgt atcagatgaa gtcattgtgc ttattgacca
    12421 tggatattca ctggccgcta ttatgacgga tattattaca acgccactgt ttaaaccgcg
    12481 aaaacaggaa aatggtgagg cagtgataaa agataacgtt attgaaatgg aaacggatga
    12541 taatggaatg aacgttatta ataaggaaga gctggatgat gtactggcca gttcggttgt
    12601 gaaatttgat gatttttcat cccggagtta atgatttacc gggcgttaaa gcccggcttt
    12661 acaagaagtg aaataacatg aatgtggaaa aatttgactg gacagaagag ttacatcaga
    12721 ccatggtgaa gagcctggtt acatcattcg ggctggattt tctgttgtta aatgatcgta
    12781 agggtggtga tgttgacact atccataatg tgcgtaatgg catttatgcc acggaggctg
    12841 agcgacagcg gtatgaaggt cgtggtgact atgattctca tcattaccac agccatgaga
    12901 actacatcgc gacgaaccgg aaaggaaagc gtgcacatca ggcagggact cttactgatg
    12961 catatacagg cgaagtgttt gcttcagacg ataacaaaaa tctggatcat attatttccg
    13021 ctaaagaaat tcatgatgat ccggggagaa ttcttgctga acgggatggt gcagaactgg
    13081 caaatgacgc ttcaaatctg gcattcacac atgagtcgct caataaatca aagaaagcag
    13141 actcaatgga tgcgtttatt tgcacactgc agaaaaacag agaagatacg ttaaggcagc
    13201 tagcagagct tgaatctcag gtaacactga cagaaaaaga aaagaaacgt ctgataagtc
    13261 tgagaaaaaa agctgatgca gatactgagc gtatggaaag ggctgataaa tatgcccggg
    13321 aaaaatacga ggccacgatt aatcagagct attacctgag tagtaagttt gctactgctg
    13381 tcgccagtgc ttcacttaat tccggttttc gtatggggac gcgacagatg ctgggactta
    13441 ttcttgccga gatctggttc gaactgaggg aacgaatccc gcatattatc agacagcaac
    13501 ggaactcatt ctgcgccggc aaatgcctgt ccgaaataac gtcagcatta cgtgcctgcc
    13561 aggaacgagt acaggaaaaa ttcagttctt ttttaattgc ctttaaagat ggtgtcatcg
    13621 gagggatact gtcaggtatt accacaacgc tgttaaatat attcctgacg acggaaagaa
    13681 tgattgtccg gcttatcagg gaaatgtgga acaacctcgt tcaggccttt aaagtcctgg
    13741 tatttaaccc ggagggatta tcgccggggc agttagccaa agcggtcact aaactgatag
    13801 cagcaggtgt ggctgttgcc ggtggggtga tgataaatga ggcgatggcg cagatcctta
    13861 tttttccatt tggttcagag cttgctgcct tttgtggtgc gctgacgaca ggtattctcg
    13921 ggctggtgat gaattattat ctcgaatata gcgaggttat gcagcgggta tgggcatttc
    13981 tggacaaatt taaggacaaa ttccagcggt cactggaata ctttcaggca gtcaatgctg
    14041 aacttgatcg ctatcttgtg gaacttactg cactggaatt ttctgttgat gctgccgcgt
    14101 taaatcattt ttcacttcat ctggatagcg taaacagtga gatagagcgc ggacttctgc
    14161 tgagagaaga agtcagccga cgagggattg cgttgccgtt tgagtcagga aatgtggctt
    14221 ctgttcgtga ctggttaaat aagcgatgaa attacaccct ccatttccct gtaaccagtg
    14281 tggggcctgt tgtcgtcatg tcaacagggc gggtgaaacg caatgtctgg atcgtggtga
    14341 cggtatttgc cgtcactatc agactgattc tcatttgtgt gcaatttatg ataaacgccc
    14401 acagatttgc cgggttgaag accagtattt actgaattat cagtctcagt acagctggca
    14461 ggagtttatt gcattaaacc aggccgcctg ccttattctg aataaactgt aatgccatta
    14521 atggcggcgt caggacagaa actgagagaa cataaaacag taaaatgtca ttccgttcag
    14581 cagccagcgt tgctttctcc ggctgctgtt attgtaaggc attttcgtta agaacgcaga
    14641 aggagaggat cgtgagattg ctgttaaaag ggctcgtcgg aatggcgctg gcctggcttg
    14701 ttattggttt cgggatggag ttattttttc tcgccgatcg ggttcatccg gcattaagag
    14761 taatcgttgg cctggggatg ttattcctga tgataatgat tgcgggtggt atgtgcggtt
    14821 ctcttctgtc acgaaagaaa acatcatgac gttcctgttt acgccgttac atttgtggcg
    14881 gcggatgatg cctgttttat gattattcag aaaaatgtat ttacctgtca cggcatgttc
    14941 agcgagcggc gccggacaga aatatcgctt ttactcaatt attatgaaaa ttaatcttcc
    15001 attcatacac tggccacgga ttagcaatga taagcgcctc gtgttatcag tggttgtctt
    15061 taccctttct aatctccttg tatcctcagg agcgcatctg atttaccgga tattattctt
    15121 tatcctggca cttcttatta ttaaggcaat gaaatcgatt acgttgcgtt tgattattgc
    15181 cttcccgttc acattactga ctgctgcaga tatcagtatc agcctgtatt catggtgtac
    15241 atttggcaca acatttaatg atggttttgc aatcagtatc cttcagacag atcctgatga
    15301 agtaataaga atgttcagga tgtatgttgt ttatgttatc gcatttatta tcctcttttt
    15361 attgttcttc tgttcagcaa taaataaaac gtcctcattg ccatcggaaa aggtcacggt
    15421 aattacattt ttattgttaa ttacagtaac gttatattct tccttccagt ttgcgttgaa
    15481 gaaacagtat caaataaatg aagttgatcc ttacatagtc gcttcccggt ttgccacata
    15541 tacccctttc tttaatctga actattttgc actggccgca aaggagtatc agagattaat
    15601 gacgattgct gataccatac ctcactacga tctggtgatt acggataaca acactgacgt
    15661 atttgttctg gtgattggtg aatctgcaag gactgataac atgtccattt acgggtattc
    15721 gcgccctacc acaccagagc ttcagaaaca gaaatcacga ctgaaactgt tcactcaggc
    15781 aatcagtggc gcaccttaca ccgcgctggc tgttccactg gcgttatctg ccgatagtgt
    15841 actgcatcat gatatccgta attaccctga taacattatc aacatggcga atcaggcggg
    15901 gttcgatacc tggtggctca gtgcgcagtc cgctttccga cagaacggaa cggctgtggc
    15961 cagtatcgcc atgcgggcca gaaacagaat ttatgtcaga ggctatgatg agttactgct
    16021 cccacacctg gctgaagcac tgaacagtaa tccggggagc aggaaactga ttgttctgca
    16081 tctgacgggc agccatgaac cggtttgcag taactggccc agagataaag ccgtgtttaa
    16141 accgctggat acagaagaag tctgttacga taattccatt cattataccg acagtttgtt
    16201 gggacaggtt ttcgccatgc tggaaacgcg tcgggcatcc gtcatgtatt tttcagatca
    16261 cggtctggag tatgacccga caaaagaaca tgcctatttt catggtggaa taaaacccag
    16321 tcagcaggct tatcatgttc cgatgtttat ctggtacagc ccgacactgg gagagcatgt
    16381 cgatcgccag acggtgaaca gcgttttttc cactgcgtat aatgattatc tgattaatgc
    16441 atggatgggc gtaaccagac cggctcaacc gaaaacgccg gaggaggtga ttacccgctg
    16501 gcaggggaaa tccgtggttt ttgatgccag tcataacgtg tttgactaca aagtattacg
    16561 aaagaacttt aatgaactcc aggaataatt cgttaaagta atttacgact ctattctttt
    16621 ttaaaggaaa ctttcagaaa atgcccccag ggcgataacc tggggaaggg ggactgtgtt
    16681 attactggtg gataaattat tacttgtttg aaagatacac taatgcaata tctactgatg
    16741 ctggatgctt gagtgtttca ttcctgctgc tgaaaacggc aatatccagg gccagacata
    16801 ttcaccgtta atatttctgc atttctgtac aatttcctca gaaagtgtct catcaattct
    16861 ttcccgcaac agcttctgct gtcttaaaat gctttcgcat atttcgaaat catcttcctg
    16921 catcagcatt tcggcgcatt taatgatttc tgctcgttca tgcagcacag aggaatggtt
    16981 atagagccag tttacaaaca gcaatgcttc cggatattgt ccccataatg gtgagtatgt
    17041 tgcatagtaa tcttctgccg tatccagaga gccggagaag ttatgccgca cagcaagtgg
    17101 aaatggatgc aaatttccgc acagcatttc cgcgatgtaa gtgtttgtag caaagccatg
    17161 acgaaaagct gtggcagcct taacatgttc cccattcaga aaatgcagaa gaccaagttc
    17221 ataccagtaa ggtgaaaatt catcagcatg ctctttcagt acactgaagg cttgcttatg
    17281 atcgcctgtt cgtaacagtt ctgagcccaa caaccagcgg gcaccctgat tatcttcagg
    17341 attgtatgcc agtattttat cagtaagcat aaccgcatcc tgatgacgct gtaagtgcac
    17401 atttgcgaga attgctgcat aaagcgcgcg cagataaggc cggttttcgg gatgcatcca
    17461 gatgatttca ccactgaaac tttccgggat gagacgatta cctgccgcca gtcctttaag
    17521 cgcggcgttc agggccttac gcggggcatt ctgctccaga aatgcatatg ccagatgagc
    17581 gtgtatatca ataaagtcag gctcccgttg agcaaggcgt ctgagtttgt tgatgtaact
    17641 cgtctcgctg atttcatcag tgtcgtatgc atcgagcact tcttcaaact cccccagtaa
    17701 tgggccaaac caggtatccg gaaatacgaa acgcccctgg ttatcgttca cttcaaagcg
    17761 taatctgtcg ttcattttct gagcctgtaa aacggatggg acacaatctg aaaagcataa
    17821 aagtcgtgat gttgtgtatc aagtcttgtt tttgtccggg atgaatgttg tttacaatat
    17881 catcggcagt ggttttacat gagaatgaaa atttatcttt atctgcattc cggtgtattg
    17941 tgaattgtcg gctgaaacgg catattaatc gattttttta ggggaatggt gctgaaataa
    18001 cgacatgatg atgatttata ttacaggtat caaaattgtt agcggtccat aacggtgtga
    18061 tgttttgaga ttg
//
LOCUS       DAZFZC010000096        17996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFZC010000096 DAZFZC010000000
VERSION     DAZFZC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.097"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            67..2295
                     /gene="crfC"
                     /locus_tag="ACHKXP_003869"
     CDS             67..2295
                     /gene="crfC"
                     /locus_tag="ACHKXP_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO7648786.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKVQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2292..3170
                     /gene="rdcB"
                     /locus_tag="ACHKXP_003870"
     CDS             2292..3170
                     /gene="rdcB"
                     /locus_tag="ACHKXP_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO7648787.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLLEKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGEHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            3434..4936
                     /gene="proP"
                     /locus_tag="ACHKXP_003871"
     CDS             3434..4936
                     /gene="proP"
                     /locus_tag="ACHKXP_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO7648788.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5048..5137
                     /gene="pmrR"
                     /locus_tag="ACHKXP_003872"
     CDS             5048..5137
                     /gene="pmrR"
                     /locus_tag="ACHKXP_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO7648789.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5113..6213)
                     /gene="pmrB"
                     /locus_tag="ACHKXP_003873"
     CDS             complement(5113..6213)
                     /gene="pmrB"
                     /locus_tag="ACHKXP_003873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO7648790.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(6214..6882)
                     /gene="basR"
                     /locus_tag="ACHKXP_003874"
     CDS             complement(6214..6882)
                     /gene="basR"
                     /locus_tag="ACHKXP_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO7648791.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6879..8522)
                     /gene="eptA"
                     /locus_tag="ACHKXP_003875"
     CDS             complement(6879..8522)
                     /gene="eptA"
                     /locus_tag="ACHKXP_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO7648792.1"
                     /translation="MLKRLLKRPSLNLFAWLLLAAFYISVCLNIAFFRQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPIACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPQMLLTLGLSGVLAAIIACWIKIKPSTSRLRSVLLRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDARRNPLMQDGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPDTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8626..9963)
                     /gene="adiC"
                     /locus_tag="ACHKXP_003876"
     CDS             complement(8626..9963)
                     /gene="adiC"
                     /locus_tag="ACHKXP_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO7648793.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFHGATYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAITT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10100..10861)
                     /gene="adiY"
                     /locus_tag="ACHKXP_003877"
     CDS             complement(10100..10861)
                     /gene="adiY"
                     /locus_tag="ACHKXP_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO7648794.1"
                     /translation="MRICSNQPCIVLLTEKDVWLRVNGKEPISLKANHMALLSCENNI
                     IDISSLNNTLVAHISHDIIKDYLRFLSKDLSQIPVWQRSATPILTLPCLMPDVFRVAA
                     RYSMSPADTESEKERTRALLFTVLSRFLDSKKFLSLLMYMLRSCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQYRERSVA"
     gene            complement(11196..13463)
                     /gene="adiA"
                     /locus_tag="ACHKXP_003878"
     CDS             complement(11196..13463)
                     /gene="adiA"
                     /locus_tag="ACHKXP_003878"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO7648795.1"
                     /translation="MKVLIVESEFLHQDTWVGSAVERLADALRQQNVTVIKSTAFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHKNVRQLIGKLHERQQNVPVFLLGDREKALAVMD
                     RSLLELVDEFAWILEDTADFIAGRAVAAIRRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMLPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLAKDNIGQKPSYCVVTNCTYDGVCYNAKKAQ
                     DLLEKTADRLHFDEAWYGYARFNPIYADHYAMRGEPGDHSGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTAEGSWFFKPWNKEVVTDPQTGKTYDFADAPTTLLTTVQE
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELADQYPD
                     TYANMGIHDLGDKMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13705..14355)
                     /gene="yjdF"
                     /locus_tag="ACHKXP_003879"
     CDS             complement(13705..14355)
                     /gene="yjdF"
                     /locus_tag="ACHKXP_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO7648796.1"
                     /translation="MTCTLKPLTLNTGALALTLILLYTGISAHDKLTWLMEVTPVIIV
                     VPLLLVTARHYPLTPLLYTLIFFHAIILMVGGQHTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALAAREILVRGVYVRGRKMTAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLTRLHCRQLRRYGLITRAPEVFNL"
     gene            complement(14478..16124)
                     /gene="fumB"
                     /locus_tag="ACHKXP_003880"
     CDS             complement(14478..16124)
                     /gene="fumB"
                     /locus_tag="ACHKXP_003880"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7648797.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVAEFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVASILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDNLPTEGNEHGQAFRDVQLEQEL
                     LKEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDEGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(16202..17542)
                     /gene="dcuB"
                     /locus_tag="ACHKXP_003881"
     CDS             complement(16202..17542)
                     /gene="dcuB"
                     /locus_tag="ACHKXP_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO7648798.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLAAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         4410 a         4391 c         4675 g         4520 t
ORIGIN      
        1 aaaacagaac tcaaaaacaa tctggctaaa taaaataaca aaatttgctt taaggaagaa
       61 ttttctatgt acacacagac cctgtatgag ttaagtcagg aggctgaacg cctgttacag
      121 ctttctcgcc aacagttgca gttactggaa aaaatgcctc tctctgtacc cggagacgac
      181 gcgccacaac tggctttacc ctggagtcag cctaatatcg ccgaacgtca cgcgatgctg
      241 aataatgagt tgcgtaaaat ttcccgactg gaaatggtgc ttgcaattgt cggtaccatg
      301 aaagcaggga aatcaaccac cattaatgcc attgttggta cggaggttct gcctaatcgt
      361 aatcgcccaa tgactgcgct gccgacgctt attcgccata cgcccgggca aaaggaaccg
      421 gtactgcatt tttcacatgt cgcgccaatc gattgtttaa ttcaacaatt acaacagcgc
      481 ctgcgtgatt gcgatattaa gcatctgacc gatgtgctgg aaatagataa agatatgcgt
      541 gcgcttatgc agcggatcga aaatggcgtc gctttcgaaa aatattatct gggtgcccag
      601 cctatttttc attgtctgaa aagtttgaat gatttagtgc gactggcgaa ggcgctggac
      661 gtcgattttc ctttttctgc ttacgccgcc attgagcata ttcccgtgat tgaagtggag
      721 tttgtccatc tagcggggct ggagagttat cccggtcagt tgacgttact ggataccccc
      781 gggccaaatg aagccgggca accgcatctg caaaaaatgc ttaaccagca gctggcacgc
      841 gcctcggcgg tactggcggt gctggattat acgcaactga aatcgatctc cgatgaagag
      901 gtccgtgagg cgattttggc ggtggggcaa tcggtgccgc tgtacgtgct ggtcaataag
      961 ttcgatcaac aggatcgtaa cagtgacgac gccgaccagg tgcgggcact gatttccggg
     1021 acgctgatga aaggctgtat tacgccacag cagatatttc cggtgtcgtc gatgtggggc
     1081 tacctggcga atcgggcgcg ctatgagtta gccaacaatg gtaagttacc accgccagag
     1141 caacaacgct gggtggaaga ttttgcccat gccgcgcttg gcaggcgctg gcgtcatgcc
     1201 gatctggcgg acctcgaaca tattcgtcat gctgccgatc agttgtggga agattcgctg
     1261 ttcgcccagc caattcaggc gttgcttcat gccgcttacg ctaacgcctc gttgtatgct
     1321 ctgcgatctg ccgcgcataa actgttgaat tacgcgcagc aggcgcggga atacctggat
     1381 tttcgtgcgc acgggttaaa cgtcgcttgt gaacaattgc ggcaaaatat ccaccagatc
     1441 gaagaaagtt tgcagctatt gcaactcaat caggcgcagg tgagcggcga gattaaacat
     1501 gaaatcgagc tggccctgac ctccgccaac cactttctgc gtcaacagca agatgcgctg
     1561 aaggtgcagt tagccgcctt gtttcaggat gattcggagc cgttaagcga gattcgtacc
     1621 cgctgtgaga cactgttaca gacggcgcag aacaccatca gtcgcgactt tacgctgcgt
     1681 tttgccgagc ttgaatccac cctttgccgg gtgttaaccg atgttattcg ccccattgag
     1741 caacaagtca aaatggaatt gagcgagtca gggtttcgtc ctgggtttca ttttcctgtt
     1801 tttcacggcg tagttcccca cttcaacact cgccagctgt tcagtgaagt catttcgcgc
     1861 caggaagcaa cggacgagca gagcacgcgt ttaggcgttg tgcgtgagac tttttcgcgc
     1921 tggttgaatc agcccgactg gggacgggga aatgagaaat caccgacaga aacggttgat
     1981 tacagtgtgt tgcaacgagc attaagcgca gaagtcgatc tctattgcca acaaatggct
     2041 aaagttctgg cagagcaggt cgatgaatct gttacggcag gcatgaatac ttttttcgct
     2101 gagttcgctt catgtttgac ggaattacag acgcgtttac gcgaaagtct ggctctgcgt
     2161 caacaaaatg aatcggtggt caggctgatg cagcagcaat tgcagcagac tgtgatgact
     2221 cacggctgga tttacaccga cgcccagctg ttacgcgatg atattcaaac acttttcacg
     2281 gcagaacgat attgaccaag acgttacttg acggccccgg tcgcgtgctg gagtcggttt
     2341 atccccgctt tttagtggat ctggcgcagg gtgatgatgc ccgccttcca caagcccatc
     2401 agcagcagtt tcgtgaacga ctgatgcagg aacttctttc gcgtgtgcag cttcagacat
     2461 ggacgaacgg cggcatgtta aatgcgccgc ttagcctgcg tctgacattg gtggaaaaac
     2521 tggcgtcgat gctggatccc ggtcatctgg cactgacgca gatcgcgcag catctggcgc
     2581 tgctgcaaaa aatggatcac cgccagcact ctgctttccc ggagctcccc cagcaaattg
     2641 ccgccttgta tgagtggttt tcagcccgtt gtcgctggaa ggaaaaggcg ttaacgcaac
     2701 gaggcctact ggtgcaggca ggtgatcaga gcgagcaaat ttttacccgc tggcgtgctg
     2761 gggcgtataa cgcctggtcg ttgcctgggc gctgttttat cgttctggag gagttgcgct
     2821 ggggggcatt tggcgatgcc tgccgtctgg gaagcccgca agcggtggcg ttgttgctgg
     2881 gtgatttgct cgagaaagcg acacaacatc tggcagagag tatcaatgcg gcaccgacca
     2941 cgcgtcacta ttaccatcag tggtttgcct cttcgaccgt tccgacgggc ggggagcatg
     3001 ctgatttttt aagttggctg ggaaagtgga ccacggcaga taaacaaccc gtttgctggt
     3061 cagtgaccca acgctggcaa actgtcgcgc tggggatgcc acgactctgt tcagcgcagc
     3121 gtctggcggg ggcaatgctc gaggaaatct tctctgtaaa tttggcgtaa ataatcagtt
     3181 acatcaatga gtcctaaacg aaatccatgt gtgaagttga tcacaaattt aaacactggt
     3241 agggtaaaaa ggtcattaac tgcccaattc aggcgtcaac tggtttgatt gtacattcct
     3301 taaccggagg gtgtaagcaa acccgctacg cttgttacag agattgcatc ctgcaattcc
     3361 cgctcccctt ttgcggccgt cgcgctgatt tttctggcgt ttgcggaaat gggccaactc
     3421 tgcgaggaaa gctatgctga aaaggaaaaa agtaaaaccg attacccttc gtgatgtcac
     3481 cattattgat gacggtaaac tgcgtaaagc cattaccgca gcatcactgg gtaatgcaat
     3541 ggaatggttc gattttggtg tttatggttt tgttgcttac gcattaggta aagttttttt
     3601 cccgggggct gaccccagcg tgcagatggt tgctgcactt gccactttct ccgttccctt
     3661 tctgattcga ccgcttggcg gactcttctt tggtatgttg ggcgataaat atggtcgcca
     3721 gaagatcctc gctatcacta ttgtgattat gtcgatcagt acgttctgta ttggcttaat
     3781 accgtcctac gacacgattg gtatttgggc accgattctg ctgttgatct gtaagatggc
     3841 acaaggtttc tcggtcggcg gtgaatatac cggggcgtcg atatttgttg cggaatactc
     3901 ccctgaccgt aaacgtggct ttatgggcag ctggctggac ttcggttcta ttgccgggtt
     3961 tgtgctgggt gcgggcgtgg tggtgttaat ttcgaccatt gtcggcgaag cgaacttcct
     4021 cgattggggc tggcgtattc cgttctttat cgctctgccg ttagggatta tcgggcttta
     4081 cctgcgccat gcgctggaag agactccggc gttccagcag catgtcgata aactggaaca
     4141 gggcgaccgt gaaggtttgc aggatggccc gaaagtctcg tttaaagaga ttgccactaa
     4201 atactggcgc agcctgttga catgtattgg tctggtaatt gccaccaacg tgacttacta
     4261 catgttgctg acctatatgc cgagttattt gtcgcataac ctgcattact ccgaagacca
     4321 cggggtgctg attattatcg ccattatgat cggtatgctg tttgtccagc cggtgatggg
     4381 cttgctgagt gaccgttttg gccgtcgtcc gtttgtgcta cttggtagtg ttgccctgtt
     4441 tgtgttggcg atcccggcgt ttattctgat taacagtaac gtcatcggcc tgatttttgc
     4501 cgggttactg atgctggcgg tgatccttaa ctgctttacg ggcgttatgg cttctacctt
     4561 gccagcgatg ttcccgacgc atatccgtta cagcgcgctg gcggcggcat ttaatatttc
     4621 ggtgctggtt gccggtctga cgccaacgct ggcggcctgg ctggtcgaaa gctcgcagaa
     4681 tctgatgatg cctgcctatt acctgatggt agtggcggtg gttggtttaa tcaccggcgt
     4741 aaccatgaaa gagacggcaa atcgtccgtt gaaaggtgcg acaccggcgg cgtcagatat
     4801 acaggaagcg aaggaaattc tcgtcgagca ttacgataat atcgagcaga aaatcgatga
     4861 tattgaccac gagattgccg atttgcaggc gaaacgtacc cgcctggtgc agcaacatcc
     4921 gcgaattgat gaataagctg aaacggatgg cctgatgtga cgctgtctta tcaggccaat
     4981 tgaactctta aggttcactt aatctctgac gcgcatactc tcctccaggt taacggagga
     5041 gagtgcaatg aaaaaccgtg tttatgaaag tttaactacc gtattcagcg tgctggtggt
     5101 cagcagcttt ctttatatct ggtttgccac gtactgatct ttcttcagcc gtacccaggc
     5161 ccgcgtgccg gaagtctctt gccggttttg caggaaaaac tgcccgtgat gcaactgtgt
     5221 aatgcggctg acaatactta accccagacc aatcccgcca taacggctgt ccatacgtac
     5281 aaacgcttta ctcaactccc cgcatttact ctcatcaata cctggtcctt catcttcaac
     5341 tgccatgacc gctccgtcat cttcttgcag cttaatcata atgttgctgc cttgcgggct
     5401 gtaacgatgg gcgttttcta ccaggtttcg caataacatc cgcagcaggg ttgcatcacc
     5461 ctgaacggtg atgtcggcgg cgctctctgg caatagcagg gtttgctgtc gctggtcgag
     5521 catggtactg agttcgtcat acgaggggag aatgacatct tccagcagtt ttacatgttg
     5581 ataattaccg gaagaaaatg actgtccggc acgcgccagt tgcagcagct gggagacgct
     5641 ctccatcatc tgatcaagcc gtgccactaa cggtgctaca tcaatgtgat gcgttttcgc
     5701 cagcagttcc agatgcaaac gcacccccgc cagtggcgtt cgcagttcgt gcgcgacgtc
     5761 agcggtaaac aacctttcgt tatccagcgt gctggtcagg cgactgacca gatcgtttaa
     5821 cgccgaaacc accgcttcga tttcgagggt ggcgctgtga atggcaatgg gcgttaagtt
     5881 gtcggcggtg cgcgcttcca gctctttttg cagctccgcc agcgggcggg tgatgcggcg
     5941 taccgcctga tagcagataa atagcgtcag gctgaccata aagacgccgg ggacaatcag
     6001 gctggcgacc gcctcgcgga tctcacgcat gatgtggcga tcgttgttgc gattgtcgcg
     6061 tagcgcctgc tcaaacagct gaatctgctc ggtactttca tgccatagcc agaagacgct
     6121 gatcagctca aacaccaaca aaatggcccc gatggtcaat atcagccgtt ggcgcagcga
     6181 tattggtcgg cgcagaaaat gcatcagatt caattagttt tcctcattcg cgaccagcat
     6241 atagccaaag ccgcgcacgg tgcggatacg ggctttgccc actttgtcgc gcagattgtg
     6301 gatatgcact tccagggtgt tggtcgaggg ttcattgtcc cagttataga tgtcgttgta
     6361 gagaatttcc cgatgcaccg gactgcctgc tttgagcatt aaccgtgaca gcagagcata
     6421 ttctttgggc gtcagaatca actcttcacc gcccatccat acctgacggc gacccatatt
     6481 cagcgtcaga ttgccaacaa tcagctcact ttcgccctga ttattatggc gtcgtagcag
     6541 ggcgcggata cgggcgtgta actcttccag cgcaaaaggc ttcaccagat agtcatcggc
     6601 accgacatcc agcccggcga ttttgtcggt cagcgtatcg cgagcggtga ggatcagtac
     6661 tggcagggtg tattttttct gccggatacg ggcgagaaaa tgcagtccat cttcgtcggg
     6721 taaccctaaa tccagtacca ccaggctgta atgaccggcc tcaaggcttt gttctgccat
     6781 ccgcgcggtt gtcacgccat cgcacgcgta gccttcggtt tgcgccgcca gaatcaatcc
     6841 ctgcaataac agcgtatcgt cttcaacaat cagaattttc attcactcac tctcctgcaa
     6901 gtttgcagaa tatcatccgc agcctggtaa tacttcgtct caacgccagt taatcccaat
     6961 agcgtggaga ataaattgtc ttgtgaatag tgttgcgttt gcgcctgttt ttgcaggcag
     7021 ttctggtcaa cctgataccg tttttgataa tcctccgaca gccacagcag cattggcacc
     7081 tgtttttggc tatccggggc gatggcataa ggcagaccgt gcagatagat gccattttca
     7141 cctaacgatt caccgtggtc agaaagataa accaggctgg tggtaaattt atcctgatgt
     7201 tctttcagca gattaatcgc tttatcaaca atatagtcga cgtaaaccag cgtgttgtcg
     7261 taagtgttca ccagttgctc tttgctacag gtctggatct cattggtgtc gcaggttggg
     7321 gtaaatttcc tgaactgcgg cggatagcgg ttgtaatagg tcggaccgtg gctgccgatg
     7381 gtgtgtaaga caatcacgcc atcaccctgc aggttattga tgtactcttc cagcccgtgg
     7441 aacagtactt cgtcatagca ttcgccgttg atgcactgac caggcagatt cagcgcggtg
     7501 acgttctggt gaggcacgcg gtcgcaggca cctttacagc cgccatcgtt gtcattccac
     7561 agcacgttga tgcccgctcg ctgaatgata tccagcacgc cttcctggtg ctgtgccagc
     7621 tcttctttgt aatgctcacg cggcatatcc gagaacatgc acggtactga aactgccgtt
     7681 gccgtgccgc aagatgcggt atcagggaaa tagaccacat tatcttttgc cagacgtgga
     7741 ttggtttcgc gtggataacc attcagtgag aagttctctg cgcgcgacgt ttctccgacg
     7801 atcaaaatgg tcaggttttt acgtttcccg tcttgcatta atgggttgcg acgagcatct
     7861 tcaccaatgc gtatcagtgg cagattagcc gaccgctggt gggaatacca cgaccaactg
     7921 gcaacaatgc tgttggacgg gcttaaggat ttcaccagct ctttgttatt gcgaaacagc
     7981 gaggcatagt ctttgtagaa cagtgccgca accagcagaa ttagtagcac ggaaatcagg
     8041 atattggctc cgcgtaaaag aacgcttcgc aaacgtgaag tggagggttt gattttgatc
     8101 cagcaggcaa taatcgcggc caacacgcca cttaatccca gcgttaacag catttgcggg
     8161 gtcatcaggg cataactttc cgctggagtc gtatcaatga tattggtgat cattgaacgg
     8221 tcgatgacga tgccataggt catgatgaaa tattgtgccg ccgcgccgac cagaataaac
     8281 aggcaagcta tcggacggtt aagccataag aaggagccta gcgtcaggac aatattaatc
     8341 acgctgaatg cgacgaccgg catcgacaag aaaaccagaa cgttatgcag tgaatccagc
     8401 ggtaacacct gcaacacctg tctaaaaaag gcgatattca ggcagactga gatataaaaa
     8461 gcagcgagta ataaccaggc gaataaattt aaagagggtc tttttagaag gcgcttcaac
     8521 atcacggtat ttccatcaaa caaagtgcgc atatgctcgc aaagcaaaat taagccaacc
     8581 ttaagttctt aaggttggct tatctgcctg cgggattaag aggcgttaat ctttgcttat
     8641 tggcgcatct aatggatacg ggtttttatg caaccggttg taattcaacg catacatggc
     8701 ggtgatcacc atcagggtga caaatgacca catgacttct tttgcgccag aaccaactac
     8761 cgcccagatg cagtagagaa aagcaatagt cgtaatggcc agatacgccg gacgtgcttt
     8821 gccgaagtgg ccgtgaccta acagcagtaa cgcggcgcag gtataaagat atggcaccag
     8881 cgtaaagata accgacacgg aagaaaccag tccaaactct ttagtggcgt ttggcgagat
     8941 gctgctgagc tggaatatcg tcattaaaat gccgacgata atcaggcctg ctactggtgt
     9001 acctgctttg tttacgcggg caaaaatcgg cgggaacagg ccgtcatcgg cggcggcttt
     9061 tgccgtttgc cccgccagca gggtccaacc gcccagtgag cctaggcaac ctgctgccgc
     9121 gcaaaaggag acaatcgcgc cggcggtgtc gcccagcgcc attcgcgccg catcaccgaa
     9181 cggcgaggca gaaacgcgca gtgccgcgtt gggtatcatc cccataattg cggtggtaga
     9241 gagtacatag caaacggcgg caatcaatac cccgccgatg gtggcgattg gcacattgcg
     9301 tttgggattt ttcaccacgc cagccgcaac ggaagcactt tctaccccaa taaatgacca
     9361 cagggtaacg ttaagggtgc tttgaatcgc gccgaaggtg cccagaccgc tgacgttcca
     9421 ggccgccata taggttgcac cgtgaaacca gaaccaaccg aatacggcaa ttccgacaat
     9481 agggatcagc gccagtacgg tggcaacggc ctgaacgcgg gtgatcattt tagggccgac
     9541 gatattcagc agaacgaaaa tccacagcac cacgacgcaa gtaatggtta ataccaatgg
     9601 atctttcaga atggggaaga aataacttaa atatcctacg ccaatgacca ccatggcgat
     9661 attaccgatc cagcaggcca gccagtagag aacgttggtt tgataaccga ggaaagggcc
     9721 aaagcagcgt cgggcatagg cgtaagagcc gccggggctg gggtcgagga acgacatttt
     9781 ggcgtatacc atcgagagtc ccagcgcacc gataattgtc accaaccatc cataaatggc
     9841 aatcccgcca gtggaggcca ggtttgcagg taacagaaaa acacctgacc ccataatatt
     9901 ccccgacacc atcagggtga cggggattaa gcccactttg tgagcatcag catccgaaga
     9961 cataattaaa ctcctgcgaa ggcgagcttc gtgacaataa attacgtcat atcatacgcc
    10021 tgcatccgcg agttgaatta ttgttggctg ggttatcgct aatgccgtta ttaatatagc
    10081 tctttgttta ggttaaaaat caggcgacag aacgttctct gtactggcta acataatgca
    10141 gcggcgtcat accatagaag tctttaaaaa cagaaataaa gtaagacgta ctgttgtaac
    10201 cgcaggactg ggatacctgg gagatatttt taccgtccat cattaattca tttacggcat
    10261 aacgcatccg gcaggtagtg attatttggc tataactggt attttcactt ttcagctttt
    10321 tctttaacaa gctagggcta aggcataaac aactggctac catactaaga ttccagtctt
    10381 tatgaatatc gctttcaata atttgataaa cgctgtcgct aacacaacta cgtaacatat
    10441 acataagtag cgaaaggaat tttttactgt cgagaaaacg tgatagcaca gtgaataata
    10501 atgcacgcgt ccgttctttt tccgactctg tatcagcggg cgacatgcta tatcgcgcag
    10561 cgacgcgaaa gacgtctggc atcaggcatg gcaaggtgag gataggggta gcgctgcgtt
    10621 gccacaccgg tatttgcgag agatctttac tcagaaaacg gagataatct ttaatgatgt
    10681 catgactaat atgagcaacc aaagtgttat taagagagga gatgtcgata atattatttt
    10741 cacaacttaa taacgccata tggttagctt tcaggctaat aggttctttc ccattcactc
    10801 ttagccagac atctttttca gtcaataaaa caatacaggg ttggttgctg caaatcctca
    10861 tacatgtact cctggatgca aataaaagac caattctcgc catagcagag cgataccggg
    10921 aaatggttat ataattgtat gatgaataaa aacatgcata aaaataacgg gttttactaa
    10981 tattacaaat ttttatagta atttcttatg tacaaaattt tttacagggt taaaaaggat
    11041 aaataacagg caaaaaaaag cgcggggtgg gaccgcgcaa aaagtatgac atttttgttt
    11101 gcagcatttt tttatgaaaa cgttgccata cttaaaaagc ctcagccaga cggcgtaatg
    11161 ttattaaaac aattacgcct gtagtggaat agtggttacg ctttcacaca cattacatgg
    11221 taaataccat caataatttc ggtaccttct gtttcgtgtt caaaaccagg gaaatggtga
    11281 tcccaggatt gtaatgagcg taaataactt acctgtggac tgtttttatc gccgaaattt
    11341 tcgccagaga gcagcattgg aattcccggt ggatacggaa taaccgagtt tgcagcaatg
    11401 cgtccaggca ggttttcaat agataccagt tcaacgttgt tatcgacgat agcgttgtag
    11461 gcctcgcgcg gagtgatttc cgccaccggc aggccggaat aggcttcgtt caatcgtgcg
    11521 ccagggttat tttctttcag ccaggcaaac attttgtcgc ccagatcgtg aatccccatg
    11581 tttgcgtaag tgtcaggata ttgatctgca agttccggca tcacctgcgc cagcggtgtg
    11641 ttcgcgtcat agtggcgttt gaaggagcag agtgtgttaa ccagtgttcc ccatttccca
    11701 cgggttacac ccatcgagaa caagaacatg atctggaagt cggtggtgcg tgtgggcaca
    11761 ataccgtggc gaccaagcca ggcggtgacc agcgctgccg ggactccggt ttcttccagt
    11821 tcaccgtctt cccccatccc cggagcgagg atgctgactt ttatcgggtc gagcatgctc
    11881 cagttatccg ggatatcttt aaagccgtgc cagctttcgc ccggatgcat cacccagcac
    11941 tcctgaacgg tggtcagcaa tgtagtcggt gcgtcggcaa agtcataggt tttgccggtt
    12001 tgtgggtcgg tgaccacttc tttgttccac ggtttaaaga accagctgcc ttcggcggta
    12061 aactctttat acaaccgcgc cattgcctga cgaaaatcaa ccgcttcatc gatcacttcc
    12121 tgagtcagtg acaggccgct gttaccgtcc atcattgata cagccacatc gtttgatgcg
    12181 cagatggcat acagcgggga agtggtggcg tgcatcatat atgcctgatt aaagcgggag
    12241 aagttaatcg caccgcggcc ttcacgcaca tgaatgtaag aggcctgcga caatgcattc
    12301 agcaatttgt gggtggaatg agtggcgaaa acggtcgggc cgctgtgatc gcccggttcg
    12361 ccgcgcatgg catagtgatc ggcatagatc gggttaaaac gcgcataacc gtaccaggcc
    12421 tcgtcaaagt gcagacgatc ggcggttttt tccagcaggt cctgcgcttt tttggcgtta
    12481 taacacacac cgtcataggt acagttggtc accacgcagt aagagggttt ttgcccaatg
    12541 ttatctttgg ccagtgggct ttcactgatt ttcttctgca aggtttcagg ttgcatttct
    12601 tgcggataga ttggtccgat aatgccgtag cggttgcggc ttggcagcat gtagaccggt
    12661 ttcgcgccag ttaacattaa gccttgctca atagatttgt gacagttacg gtcgacgacg
    12721 acgacatcgt tatcggtcat acaggcttgc atgatggtgc ggttagagcc ggaagtaccg
    12781 acgaccaccg accaggagcg atcggcgcca aatacgcgcg cggcatattt ttcgctttcg
    12841 ccaaatgcgc cagtatggtc aagcaaagaa ccgagggatg ttctttcgat ccccatgtcg
    12901 gtgcggaaca gattttcacc atagtagtca tgatagaaac gtccggcagg cgttttggtg
    12961 aaaccgacgc cgccctggtg acccggagcg gcccaggaat attcatggat gtcgctgtat
    13021 ttcatgagcg cgctgaacag tggcggcaat agttgctgac ggtagcgacg gattgcggca
    13081 acggcgcgtc cggcaataaa gtcagcggta tcttccagaa tccaggcgaa ttcatcgaca
    13141 agctccagca gagagcgatc catgacggcg agggcttttt cccggtcgcc taacaggaag
    13201 acgggcacgt tttgttggcg ctcatgaagc ttaccgatca attgtctgac gtttttatgt
    13261 tcgtctggat gttccatttg atagctgaac atcaggcagt caatggcttc gttcgaagag
    13321 agaatggcaa aaccatcatc aaaggcggtt gatttaatta cggtaacatt ttgctgtctt
    13381 aaggcatccg ccagacgctc tacggcgcta ccgacccagg tgtcctgatg cagaaactcg
    13441 ctttcaacaa ttaatacttt catcattgct tacccggtta tgaaggaatc ttcgttgcgg
    13501 gcaagtatct tccggccatt tcaggctgta aagcgcatga aaaatggacg atattgtatt
    13561 tttgttttta aaaacaaaat attatgttcg tttttgaatt aaattttatt gcttattatc
    13621 gacagcaaaa ctattttttt ggctgatact gatatcgtct gtagccggga agcccggcga
    13681 ctggctgccg ggcgggttgc gccgctaaag attgaacact tctggcgctc tggtgattaa
    13741 gccatagcgc cgtaactggc ggcagtgaag acgagtgagg aatatcaccg tggttaatgc
    13801 gcccagcagc gcgcagaaca tatcggattg cgtatcccac ggatcgccct gagtaccgag
    13861 aaaatcatcc gctccctggc ccatcgccag tgccgcccac cactcgatca actcatacat
    13921 cgcgcttatc gccagtgcta cacagcaaac cagaaatgcc gtcattttgc gtccgcgaac
    13981 gtataccccg cgcacaagta tttctcgtgc cgccagtgca ggcaccagcc cctggaaaaa
    14041 atgtcccagc ttgtcatacg gattacggct cagccccagc cattcctgaa cttcaaaacc
    14101 gatggggact tttgcgtagg tgtgctgacc tccgaccatc aggatgatgg cgtgaaagaa
    14161 aatcagcgta tagagcagtg gggttaacgg gtaatgtctg gctgtaacaa gcagtagcgg
    14221 aaccacgata atcaccggcg ttacttccat caaccaggtg agtttgtcat gggccgatat
    14281 acccgtataa agcagaatta acgttagcgc cagcgcgcca gtattgaggg ttaacggctt
    14341 gagtgtgcag gtcatgatga gttcacaagt caggaaaagt actgactatt caccagtaag
    14401 catgaaatta caattgcaga agaggtggcc cggggggatc acctggcagc acgccgccag
    14461 gcgccgggcc gaagaggtta tttagtgcag tttgcgcact gtttgttgac gatttgctgg
    14521 aagaagtcgt tgcctttgtc atcgaccagg ataaacgccg ggaaatcttc cacttcgatt
    14581 ttccagatag cttccattcc cagttctgga taagcaacgc attccagatg cttaatgctc
    14641 tgctgcgcca gcacagctgc cgggccgccg atgctgccga ggtagaagcc gccgtgttta
    14701 tggcacgcgt cggtaacctg ctgactgcgg ttgcctttcg ccagcatgat catgctaccg
    14761 ccgtgggatt gcagtagatc cacgtaagag tccatacggc ctgccgtggt tggaccgagt
    14821 gaaccggaag gataaccggc tggtgttttt gccgggcctg cgtagtagat cggatgatct
    14881 ttaatgtact gcggtagctc tttaccttcg tcaatcagtt ctttcagttt ggcatgtgca
    14941 atatcacgac ccacgataat tgtgccggtg agtgacagac gagtagaaac cggatattgc
    15001 gaaagttggg cgaggatctc tttcattggg cggttgagat cgacttttac cgcttcgcct
    15061 tcgcccgcct gacgtagctc ttcaggaata tactggcctg gattgtgttc cagtttttcg
    15121 atccagatcc cttcacggtt aattttcgct ttgatgttac ggtctgcgga gcaggagacg
    15181 cccataccaa ccggacagga tgcgccatga cgcggtagac gaatcacgcg aatatcgtga
    15241 gcaaagtatt taccaccaaa ctgcgcgccg aggccgagtt tctgagcctc tttcagcagt
    15301 tcctgttcca gttgcacatc gcggaaggcc tgaccgtgtt cattaccttc agtcggtaaa
    15361 ttgtcgtaat agtgagcgct tgctaacttg acggttttca ggttggtttc cgcagacgta
    15421 ccgccaatca caaacgcgat atggtacggc gggcaggctg cggtgccgag agtccgcatt
    15481 ttctcaacga ggaaattctt cagcttgccg ggagtcagca gggctttggt ttcctgatag
    15541 agatacgttt tgttagcaga gccgccgcct ttagcgacgc agaggaattt gtactcgtca
    15601 ccatctaccg catacagatc gatttgcgca ggcaggttag tgccggtgtt aacctctttg
    15661 tacatatcca gtggcgcatt ttgtgaatag cgcaggttat cttcgatata agtgttataa
    15721 acgcctttcg acagcgcttc ttcatcaccg ccgccggtcc acacgcgctg gccttttttg
    15781 ccgacgatga tggcggtacc ggtatcctgg caggttggca gcacgccttt ggcggcgatt
    15841 tctgagtttc ttaaaaattg cagcgccaca tacttatcgt tttcactggc ttctgggtca
    15901 tgaagaatag aagcaacctg tttctggtgt gccgggcgaa gcatgaaaga agcgtcgtgg
    15961 aaggcttgct gcgccagcaa agtcagagct tcaggctcta ctttcaggat agtttcgccg
    16021 tcgaattcgg caacgctgac gtaatcggat gtgagcagat agtattcggt attgtctttc
    16081 cccatcggga aaggcgcctg gtagataaac ggtttgtttg acatagcttc cagcctgtaa
    16141 ctctgtattt gttatacgaa aatggcgtgc cgcacaacga cgggcggcac gcagagtgca
    16201 attacaagaa cccgtacatt gcggcgaaga tccagccaaa gacgcaggat acgcttacgc
    16261 caatcaaccc tggcagaatg aagctatggt tgatcacgaa gcgaccgatg tgggtggtgc
    16321 cggaacggtc aaactgaatt gccgccaggt cgctcggata ggtcggcagg atgtaataac
    16381 cgtagcaagc cggtgctgaa gccacgatgt acgcagggtc aacgccaatc gccagcgcga
    16441 ccggaacaat cgccgccagc gctgcggcct gggagtttac aaacttggag accagtagca
    16501 gaacaatggc ataagcccac ggatactctt tcaccatttc acccagtacg ccctggattt
    16561 cagacatatg cgcaccgaac atggtttccg ccatccacgc aataccgtac accgccacaa
    16621 tggcgatcat accggaacgg aagacttcgt tttttgagat agacgcggga ttggttttcg
    16681 tcaggataat gatcagcgcc ccggttagca gcataaacat ctgaatgacc agtaccatcg
    16741 acagcggttt gccgccgaaa gatggacgca ggtcagaatc tgcaccgagc agagcgacca
    16801 cggcgattgc cgcgaggaag atccacattg ccagccagtt actctttggc agttttttat
    16861 ccagcagcgt ggcggtatca ccgtaaacat attcgcggtt ttctggaacg gagatgaatt
    16921 tctggaactc ttcatcttta tccagatctt taccgcggaa ccagctaaag ataccgatcg
    16981 ccaggatacc gatcagggtt gatggaatgg tgatggcaag caggtcgagg aattcaagat
    17041 gacgcccatc aaaggtgacg ttacccagca tcgcgaccag cgataccacg gcaacggata
    17101 ccgggctggc gataatcccc atttgcgcgc cgatagaact cgccgccatc ggacgctccg
    17161 ggcgaatgtt gttcttaata gcaacgtcgt agatgatagg cagaatggtg tagaccacgt
    17221 gacccgtacc acaaagaatg gtcagcgtac aggttacaaa aggcgcgacg atagagacat
    17281 atttcgggtt gcggcgcagc agtttctcag cgatttgcag catcacatca aggccgcccg
    17341 aggcttgcaa ggttgccgat gccgccacca ccgcaatgat aaccagcata acatcaaccg
    17401 gtggtttacc cggctgaagg tggaagacga ataccagaat gaccaggccg ataccgccta
    17461 ataaacccag cgcgataccg ccctttctgg caccataaaa cagacatatc agtattatga
    17521 tgagctgaat agtaaataac atgtgtgaac cctcgcgata atcccattta aatttttgca
    17581 gaatagatca cagtcacgtt ctggtttgta tgaactgttt tcagagcctg actaaatatc
    17641 cgtttggtcg ttactggctt aagcaaacac ctcacagtga atattgaccg atttacgcaa
    17701 tggtaaattt atgcactgcg ataagtgatt ttttcagaga taaatactct ctggcgatgg
    17761 gtttgttgtt tgtacttcgc agatgttaat ggtttgtttg cttgatatct tattgatatt
    17821 tattggtctg gttttgttct acaggcagaa accgctaagt ttatagatta ttctgatcta
    17881 cctatttgtg ggtaaaaata cacatattgc gggtgacata atagttaatt aacttttgtt
    17941 agcgttttga aattaaaaac actattcatc cgaagggata ttaatttcag gaacag
//
LOCUS       DAZFZC010000097        17375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFZC010000097 DAZFZC010000000
VERSION     DAZFZC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.098"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(239..1240)
                     /locus_tag="ACHKXP_003882"
     CDS             complement(239..1240)
                     /locus_tag="ACHKXP_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7648799.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1347..1643
                     /locus_tag="ACHKXP_003883"
     CDS             1347..1643
                     /locus_tag="ACHKXP_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7648800.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1777..2202)
                     /gene="arsC"
                     /locus_tag="ACHKXP_003884"
     CDS             complement(1777..2202)
                     /gene="arsC"
                     /locus_tag="ACHKXP_003884"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO7648801.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2215..3504)
                     /gene="arsB"
                     /locus_tag="ACHKXP_003885"
     CDS             complement(2215..3504)
                     /gene="arsB"
                     /locus_tag="ACHKXP_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO7648802.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3558..3911)
                     /gene="arsR"
                     /locus_tag="ACHKXP_003886"
     CDS             complement(3558..3911)
                     /gene="arsR"
                     /locus_tag="ACHKXP_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO7648803.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4651..4734
                     /gene="dinQ"
                     /locus_tag="ACHKXP_003887"
     CDS             4651..4734
                     /gene="dinQ"
                     /locus_tag="ACHKXP_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO7648804.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4788..6140)
                     /gene="gorA"
                     /locus_tag="ACHKXP_003888"
     CDS             complement(4788..6140)
                     /gene="gorA"
                     /locus_tag="ACHKXP_003888"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO7648805.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6212..7054)
                     /gene="rlmJ"
                     /locus_tag="ACHKXP_003889"
     CDS             complement(6212..7054)
                     /gene="rlmJ"
                     /locus_tag="ACHKXP_003889"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO7648806.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARLLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGIYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7257..9299
                     /gene="prlC"
                     /locus_tag="ACHKXP_003890"
     CDS             7257..9299
                     /gene="prlC"
                     /locus_tag="ACHKXP_003890"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO7648807.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATMNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9307..10059
                     /gene="rsmJ"
                     /locus_tag="ACHKXP_003891"
     CDS             9307..10059
                     /gene="rsmJ"
                     /locus_tag="ACHKXP_003891"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO7648808.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10108..11577)
                     /gene="dtpB"
                     /locus_tag="ACHKXP_003892"
     CDS             complement(10108..11577)
                     /gene="dtpB"
                     /locus_tag="ACHKXP_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO7648809.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSVVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11743..11796
                     /locus_tag="ACHKXP_003893"
     CDS             11743..11796
                     /locus_tag="ACHKXP_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648810.1"
                     /translation="MVLNVVLAGDFDCVRRP"
     gene            11774..11827
                     /locus_tag="ACHKXP_003894"
     CDS             11774..11827
                     /locus_tag="ACHKXP_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648811.1"
                     /translation="MIAYADRRPDKVFTLIR"
     gene            complement(11894..12328)
                     /gene="uspA"
                     /locus_tag="ACHKXP_003895"
     CDS             complement(11894..12328)
                     /gene="uspA"
                     /locus_tag="ACHKXP_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO7648812.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12719..13054
                     /gene="uspB"
                     /locus_tag="ACHKXP_003896"
     CDS             12719..13054
                     /gene="uspB"
                     /locus_tag="ACHKXP_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO7648813.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13298..14797)
                     /gene="pitA"
                     /locus_tag="ACHKXP_003897"
     CDS             complement(13298..14797)
                     /gene="pitA"
                     /locus_tag="ACHKXP_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO7648814.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15029..16231
                     /gene="yhiN"
                     /locus_tag="ACHKXP_003898"
     CDS             15029..16231
                     /gene="yhiN"
                     /locus_tag="ACHKXP_003898"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO7648815.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAVRGQ"
     gene            16374..17216
                     /locus_tag="ACHKXP_003899"
     CDS             16374..17216
                     /locus_tag="ACHKXP_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFO7648816.1"
                     /translation="MARGRRLKSYLDYENALGDGIGVGYGQSYQPWLRAQDVKSRGNR
                     SIVFGLKTFRNHHLLSSVESNFFYLAEFNDSVIDIREQFPLFPLRLTQQIANHLHFQH
                     PMVRGVRGVPVEVLNVMTTDFLLTLRTPEGGLRYKAIAVKHNESIPEREAQKLEIERM
                     FWQLIDVEFQIYVGSELNNVVGKNICWATSVLRDGSEFYDKYPLDKILWKLKPDVYPI
                     VGLRAMISSIFGVDAQEAMMLLQAMIGLKMINVDLSYPILETGLIKIISNDHYIGLNA
                     NGYY"
BASE COUNT         4484 a         4334 c         4462 g         4095 t
ORIGIN      
        1 tctgcctttt aaaaaggagg agggtactga gacaaagagt cattgcttct ccgataaaat
       61 gtcagagaag catgattacg tggttattgt ctgttgatga cttgttgacc gagccgtcat
      121 cattctcaat gcattagtta aatcatttgt gatttttaag gattatcctg tctatgattt
      181 tctacttaga aggatatggc actaactatg catcttatag tttactgctg gcatctattt
      241 aaaatagcaa ttgagtcaaa tagcccatca agatagccat acctaatata accgtcagga
      301 atgcagctat catcggcatt ctgaacattg atttaagtaa tatcacttcc gtcaggcttg
      361 cgccggcact gccgatgatt aatgccatta atgctcccag acccattcct tttgtcatca
      421 gaacagatgc cagagggata actgcctcag cccggatata cagcggaata ccgacaacgg
      481 cgcttaatgg gatggcgaag ggattatctg ctcctgcatg agcggcaatc cacgctgagg
      541 gaataaagcc ataaataaaa gatcctatta atactcctaa caaaagatat ggcagtacat
      601 ctttaaactg ttgcaacgca tctttggtgg ctagttttat gggattaata cttactacag
      661 ctttggtact gcaacaggta actacggggt tctcaatggc tttagccgct ggactacaac
      721 aactcacttt tatgggcgta tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat
      781 ttgctgatga gcttttactg gcaatgatat gacgttcaaa ccccagagaa tccaggataa
      841 tactggcaag aacggagacg ccggctgcga taatcgcgta caacaaggtg actttccaac
      901 caaaggtcat ccacattagt ccgacgataa tgggattaag taatggtgaa acaaataaaa
      961 aagtgagagt cggaccaaac ccggctttcg ctgacaacaa tccacgtaac atgggtattg
     1021 tcgagcaact acagaacggg gtaacggctc ccaacagagc agccaggaga tagcctctcc
     1081 cttttctggc ccccatcatc tgctggattt tatggtctgg caccttttgt cttatcaggc
     1141 tgacaccggc actaatcact ataaacagca acgaaagctc gacggcgaga aacacaaaca
     1201 tctctgcggc atcttgcagc atcgcaagcc aactactcat acgtaacctc attggataaa
     1261 tctatatttc cagaataatc gaaatgaaat tcagatcaag atatttctgg tattatcgaa
     1321 atatcaacca gacagagggg tgtcatatgc agttggaaga agtcgctaaa gcgctgaaag
     1381 agctaggtca cccgacccgt ttgttcatat ttaaacatct tgtcaaagcc ggggaacaag
     1441 ggttgccggt cggtgagttg caaaagcagc ttgggatacc tggctccaca ctcagccacc
     1501 atatatcagc actggtatcg gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga
     1561 tgtgtgtttc gcaatacgca atgctggaag cgataattga attcttacgc gaggagtgct
     1621 gcgtaaatag caaaattgcc tgatgcgcta cgctctacat atttcatgcc atatattgaa
     1681 tttgcacggt tttgtaggtc ggataagacg cgttagcgtc gcatccgaca taaacaaagc
     1741 gcactttacc aactatcggc agtggatatc gccgctttat ttcaggcgct tacccgcttc
     1801 atcaaccact ttctcgccat cttccttgga gaatgcgcct ttttgcgcat ctggcagaat
     1861 ttccagcacc acttctgaag ggcggcacag gcgagttccc agcggcgtca ccacaatcgg
     1921 gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt
     1981 atcttccgca aggcccagct cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac
     2041 ggaaatcccc atatcggcaa tgagtttgac cagttcatcg cgcgttggcg gagtttccag
     2101 ataatggata atggtcggtt ctgtgccgct gttgcggatc atctccagcg tattacgcga
     2161 cgtgccgcag gccgggttgt gataaatggt aatgttgctc atatcagtat ctcattacaa
     2221 agtgaaagag agacgtagcg ccagcgcagc cagcgtcaca aacagcacag gcagggtcat
     2281 gataatccct gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac
     2341 gtgcagccag agtagcgtag ccaggctacc aattggggta attttcggtc ccaaatcgca
     2401 gccaatcaca ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc
     2461 aatggacaac gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc
     2521 ggtgaggaat ccggtgccga gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag
     2581 tacgccagaa agatattccg ttaatccggc attgcgcagg ccataaacca ccagatacat
     2641 gccgagcgag aagatgacaa tctgccaggg ggcaccgcgc aggactttac ccgtattaat
     2701 cgcatgaccg cgtttagcga cgacaaataa tatcagcgcg cccacagctg caatggcgct
     2761 caccggaatg ccgagcggtt ccaggacgaa aaatcccacc agcagaagca gtaaaacaac
     2821 ccagccagtt ttgaacgtag caggatcttt gatcgcttct gcgggagatt tcagcagcgc
     2881 catatcgtag ttctgcggaa tatctttgcg aaaatagaga tgtaacatca ccagcgtggc
     2941 aacaatcgcg gcgatatcca ccggcaccat caccgaggcg tattcgcgaa agccgaggcc
     3001 aaagaaatca gcggaaacga tattcaccag gttggagaca ataagcggca ggctggcggt
     3061 atcggcaatg aatccggccg ccatcacgaa cgccagcgta gtgcctttac tgaaccctaa
     3121 agccagcagc atggcgatga caatcggtgt caaaataagc gccgcgccat cattggcaaa
     3181 cagggcggca acggcagcac cgagcaggac aatccaggta aacagcaagc gaccacgacc
     3241 attaccccag cgtgagacgt gcagcgccgc ccattcaaaa aagccggact catccagcag
     3301 caggctgatg ataatgacgg cgataaacgc agccgtcgcg ttccagacga tattccacac
     3361 caccggaata tcacccggat ggaccacgcc cgtaactaac gccagtactg cgccgagcgt
     3421 tgcactccag ccgatgccta aacctttcgg ctgccagata accaatacga tggtcaggac
     3481 aaagatagcg cctgccagta acataatgcc tcccggataa aacacatctg aaaattcata
     3541 tgtgtttagc taaattttta actgcaaatg ttcttactgt ccccggaaca gttttgtcga
     3601 gccaggttgc ggacaatcgc ctgaaccttt tcctgttcac atcgccaggc ctcatcaata
     3661 attttcgccg cccatgctgg aatatgcggt gataagcggt aatgaaccca cttaccttgc
     3721 ttgcggtcca gcaatagccc gctttcacgc agcaatgcca ggtggcggga gatcttgggc
     3781 tgcgactggt cgagagcagt gcagagatcg cagacgcata actctcccag ttcgctgagc
     3841 agtaaaacga tgcccagacg ggtttcatca gcaagaattt tgaacaattg gatgggtaac
     3901 agaaatgaca tattgcgctc ctgattgttg caggtagtgt ctctcttcga agcggataag
     3961 tcaaaaacat atatgactta acgaatgtgt aagtgcaggg gaaggtaata ggtgtgaatt
     4021 ttgagttggc tattcatttg aaaggaggtc tgaatcagga cgtaaaaaaa ccgactttgc
     4081 gtcggttttt tactttccag ccctgagttg gtggctctgg ctggagtgag agagttctta
     4141 tctaacagct caatactatt agccagtggc taacattgca agaattagcg atttcttcag
     4201 ctggcggagg gggcacttcg gtatatatcg caatgacgat ttatatactt tcactgggtc
     4261 atcgtcatat taagccttgt ccgaatccgg atgtaaaaaa accgactttg cgtcggtttt
     4321 ttactttcca gccctgagtt ggtggcactg gctggagtga gaatgccatt atctaacgca
     4381 tcaatgctaa taccatactg aaactattgc aaggacatgc tggttttata acctgaatgt
     4441 actgtatgat tatccagtta gctctgaggc attttcactc tggtaatgcg cataaacgct
     4501 ttcaaagtcc tggtcagaag tacgggtggt gccgttaact gatgctctgg ccggagtgag
     4561 aatgccatta tctaacaata gggcatgcgc cgtgacaggc agtggatgag taagcggatg
     4621 cattctcact ccatcgcatg gagaaaacgg gtgattgata aagcaatcat cgttcttggg
     4681 gcgttaattg cgctgctgga actgatccgc tttctgcttc agcttctgaa ctgatagcgg
     4741 aaacgtaatt aagggctaag agcacactac tcttagccct ttaacattta acgcattgtc
     4801 acgaactctt ctgccgccgt tgggtgaatg gcgacggtat tgtcgaagtc ttttttggtt
     4861 gcccccatct tcagcgccac cgcgaagccc tgcaacattt cgtccatacc aaagccaatg
     4921 ccgtgaatac cgacaatctt ctcttccgat ccaacgcaca ccagcttcat gcggcacggc
     4981 tggcggtgag tggtgacggc ggtatacatc gcggtgaaag aggatttata cactttcacc
     5041 tgatcgtcgc catactgctc gcgcgcctgc ggttccgtta aaccaacagt accaatcggc
     5101 ggatggctga agaccacggt cggaatgttg ctgtaatcca gatgctcatc cggcttgtta
     5161 ttaaacaggc gttcagagag acggcgaccc gctgcaactg ccaccggtgt cagctccact
     5221 gcacccgtgt tatcgcccac cgcgtaaata ccttcaatat tggtgttttg atatttatcg
     5281 acgacgatat agcctttttc gttagtttta acgccagcgg cttccaggtt gatgttgtca
     5341 ttggcaggct cgcgaccaat cgcccaaatc aggcaatcca ccgtttcact gcgaccatct
     5401 tccagctcca gcgtcaggct accatcggca tttttcacta ccgctttcgg gatggcgttg
     5461 gtgtgcagct gcgggccttc ggcgttcatc acttcgacca gcgtttcgga aatcatcggg
     5521 tcgaagctgc gcagcggcgc atgtttacgc acaaacagat gcgttttcgc gccgaggccg
     5581 ttaatcacgc ccgccagctc aacggcgatg taacccgcgc caacaaccgc cacgcgctct
     5641 ggcaaagcag gaagggcgaa gaagccatca gaatcaatac cgtattccac gcccggaata
     5701 tccgggtggc tcggacgacc gcctgtggcg atcagaatat gatcggccgt gatggtttcg
     5761 ccgtttacct ccagcgtttt ggcatcaacg aagcgggcaa agcctttgat tacatcaacg
     5821 ttatttttac cgagcacgtt ttcataggaa gtatgaatac ggtcgatata ggcggtacgg
     5881 ctggcgatca acgtttccca gttgaattta ttgatagtgg tatcaaaacc ataatccggg
     5941 ccgtacatat ggatcgcttc acggatttgc gccgcgtgcc acatcacttt tttcggcaca
     6001 cagccaacat ttacgcaggt gccgcccagc tctttggctt caatcagcgc acatttctgg
     6061 ccgtacatag ccgcgcggtt gatggaggcg ataccgccgc tgccgccgcc gatggcgatg
     6121 taatcatagt gtttagtcat gacaaagtgt ccttatcgtt gattaccgcg attgtagcgc
     6181 gagacgtaat aggtgccagc aatggctgca attactccgg cacgatccag cttacggtgg
     6241 cgtgcccggt gcctgccgga accagtttgc tgtgcagcca cggcagcacg ttattcatct
     6301 gttgttccag tttccacggc gggttaatca caatcatgcc ggaagcggtc atgccacggc
     6361 gatcgctgtc tggcagtacc gccagttcaa tttgcagaat tttgcgaata ccggtcgctt
     6421 ccagatcgtg gatcatgcgc ttaatttgct gacgcagcac caccggatac cacagtgcgt
     6481 aaataccagt ggcgaaacgt ttgtaacctt ctgctatccc gctgaccacc gcttgatagt
     6541 cagttttcat ttcatacggc gggtcgataa ggattaaacc acggcgggaa accggcggca
     6601 gtttggcctt aagctgctgg aaaccgtcgg ctttttcgac acgcgcacga ctatctttct
     6661 gaaattcaga acgcaacaac gggtaatcgc tcgggtgcag ttcggtcagt tgcaggctgt
     6721 cctgttcacg cagtagcagg cgagcaatca acggcgaacc ggggtagtaa cgcaactgac
     6781 cgctacggtt gaagtgtttt accacattga tgtacgcctc cagttctgcg ggcaaatcgt
     6841 cctgctgcca gatacgggcg atgccttcga gatattcgcc ggtacgctcg gcatgttcgc
     6901 tgcctaactg ataacgcccg gcccctgcgt gggtgtcgag atagagaaac ggtttatctt
     6961 tctctttcag cgactcgatg atcaggctct gaacggtatg tttaaggacg tcggcgtggt
     7021 tgccagcgtg aaagctgtgg cgataactga gcatgggtaa aggtgttccg gtaagtaaaa
     7081 atcggcccgt attcggggcg cacaaaagcg tatcaggaca gtataccgaa aagaggccgc
     7141 cgccgcgaaa gcgtaacgtt tctcattgaa attcactaca cttaacccca tgctacacac
     7201 attatgtaaa gcgcctgttg agcgcttcct taacctcttt aaccaggact gcgcgaatga
     7261 cgaatccgtt actgactccc tttgaattgc ctccgttttc taaaattctc ccggaacatg
     7321 tcgttccagc cgtgactaag gcattgaacg actgccgcga aaacgtggag cgcgtagtag
     7381 cgcaaggggc accgtacacc tgggaaaatc tctgccagcc gctggcggaa gtggacgatg
     7441 tgttggggcg tatcttctcc ccggtcagcc acctgaactc ggtgaaaaat agcccggaac
     7501 tgcgtgaagc ctacgaacaa accctgccgc tgctgtcgga atacagcacc tgggtagggc
     7561 aacatgaagg gctgtataaa gcgtatcgcg acctgcgcga tggcgatcat tacgccacga
     7621 tgaacacggc gcagaagaaa gcggttgata acgcactgcg cgactttgaa ctctctggca
     7681 taggtctgcc gaaagagaaa cagcagcgtt acggcgaaat tgcgacccgt ctttctgaac
     7741 tgggcaacca gtacagcaac aacgtcctcg atgcgacaat gggctggacc aaactcgtta
     7801 ccgacgaagc ggagctggcg gggatgccag aaagcgcgct ggctgcggca aaagcccagg
     7861 ccgaagcgaa agagctggaa ggttatttgc tgacgctgga tatcccaagc tacttgccgg
     7921 taatgaccta ctgcgacaac caggctctgc gtgaagagat gtatcgcgct tacagcaccc
     7981 gcgcctccga tcaaggccca aacgccggta aatgggataa cagcaaggtg atggaagaga
     8041 tcctcgcgct gcgtcacgaa ctggcgcaac tgctgggctt tgaaaactac gcctttaaat
     8101 cccttgccac taaaatggca gaaaacccgc agcaggtgct ggatttctta accgatctgg
     8161 caaaacgcgc gcgtccacaa ggcgaaaaag agctggcgca attgcgtgcc tttgccaaag
     8221 ccgaatttgg cgtcgatgag ttgcagccgt gggatatcgc ttactacagc gaaaaacaaa
     8281 aacagcacct ctacagcatc agtgacgaac agctgcgtcc gtacttcccg gaaaacaaag
     8341 cggttaacgg cctgtttgaa gtggttaagc gtatttacgg catcaccgct aaagagcgta
     8401 aagatgttga tgtctggcat ccggatgtac gtttcttcga actgtatgac gaaaataacg
     8461 aactgcgcgg tagcttctac ctcgatctgt atgcccgtga aaacaagcgc ggcggggcgt
     8521 ggatggatga ctgcgtaggc cagatgcgta aagctgatgg ttctttgcaa aaaccggtcg
     8581 cgtatttgac ttgtaacttc aaccgcccgg taaatggtaa accggcgctg ttcactcacg
     8641 acgaagtgat caccctcttc cacgagttcg gtcacggcct gcaccatatg ctgacccgca
     8701 tcgaaaccgc tggtgtttcc ggtatcagcg gtgtgccgtg ggatgcggtc gaactgccga
     8761 gtcagtttat ggaaaactgg tgctgggagc cggaggcgct ggcgtttatc tctggtcact
     8821 atgaaaccgg cgaaccgctg ccgaaagagt tgctggataa aatgctggcg gcgaagaact
     8881 accaggcggc gctgtttatt ctgcgtcagc tggagttcgg cctgtttgat ttccgccttc
     8941 atgccgagtt ccgcccggat cagggggcaa aaatcctcga aactctggca gaaatcaaga
     9001 aactggttgc cgtggtgcca tctccgtcct ggggccgttt cccgcacgct ttcagccata
     9061 ttttcgccgg tggttatgcc gcaggttact acagctacct gtgggctgac gtactggcgg
     9121 cagatgcttt ctcgcgcttt gaggaagagg gcattttcaa ccgtgaaacc gggcagtcgt
     9181 tcctcgacaa cattctgagc cgtggcggtt cagaagagcc gatggatctg ttcaaacgct
     9241 tccgtggtcg tgaaccgcag ctggatgcga tgctggagca ttacggcatt aagggctgat
     9301 cattcagtga aaatctgctt aattgatgaa acaggcaccg gagacggtgc cttatctgtt
     9361 ctggcggccc gctgggggct ggagcacgat gaagacaacc tgatggcgct ggtgttaacg
     9421 ccggaacatc tggaattgcg caagcgtgat gagccaaaac ttggcggcat ctttgttgat
     9481 tttgttggcg gagcgatggc gcaccgacgc aaattcggcg gtggtcgcgg tgaggcggtg
     9541 gcgaaagcgg tgggcattaa aggcgattat ttgccggatg tggtggatgc cactgcagga
     9601 cttgggcgcg atgcctttgt actggcttca gtcggctgcc gcgtgcggat gctggagcgt
     9661 aatccagtgg ttgccgcgct actcgacgac ggcctggcgc gtggttatgc ggatgcggaa
     9721 atcggcggct ggttgcagga gcggttgcag ttaattcacg cctccagcct gacggcgctg
     9781 actgatatta ccccgcgccc gcaggtggtt tatctcgacc cgatgttccc gcataagcag
     9841 aaaagcgcgc tagtgaaaaa agagatgcgt gtctttcagt cgctggtggg accggatctt
     9901 gatgccgatg gattactgga gcctgcacgc ttgctggcga ccaaacgcgt ggtggtaaag
     9961 cgcccggact acgcgccacc gctggcgaat gtcgccacgc caaacgcggt agtcactaaa
    10021 gggcatcgct ttgatatcta tgcaggtacg cccgtgtaaa agtaaagcag ggtagcgata
    10081 acgctaccct gctttctgca agaataatta atggctttcc ggcgtcgcaa tcatgcgttt
    10141 cagccacggc accatcaaca gcatcactac tgcaacgccc agcgtgacca gaccaatctt
    10201 accaaacacg ttggtataga cgggcaacgt ctcaagcgga tcggtaatgt tgtccggcac
    10261 cgcggtaaat gttgccacat agccgcccag caagaacgcg gcagcctgcg tcaggaacca
    10321 catcccgaga ataaagccca tcaaatgctg cggcaccagg gcagcaatca tcgccaggcc
    10381 aagggcgcta ataaacagtt cacctaagct ctggaataag tacaccagca cgataaacca
    10441 tggcgatgtc agcccttggg catccgcaaa ccacattccc gcagctgcag ctgtcaaaaa
    10501 gcccagtgag cacataaaca tgccgagagt aaatttcatc ggcatcgaga ggtctttgcc
    10561 tttgttaccc agatgcgtgt aaatgcctgc cagtattggg ctggcgagta ccacccagaa
    10621 cgggttaagc gcctggaagc tgaccgggtt gatggaaaag ccgagaattt catgatgcac
    10681 gttgttgatg gcaaagaagt tcagcgacgt tggcatctgg gcgtagagaa tgtaaaacac
    10741 caccgcttcg agcatcagga caaaggcgac aaacatttta ttgcgcccgg ttttatccag
    10801 cttgaatgcc tgacgaaaga agatgatggt gacgacgacg gaaagaacaa tcagcaccag
    10861 attggcgact tctacgttgt gcatcagcca tgcgcatacg aagatcatca ccacgctgcc
    10921 aagtaacacg tacaacagtt tgctgaagct cattggccgg aagtcgggtt cagaaccaat
    10981 gtctttcacc attccacgac aggcgatgta aaccagtaat gcgataatta accccgcccc
    11041 gcacaggttg taggtgactg aataaccgaa tctatcagcg atcacagggg ccagcgataa
    11101 cgctatcaac gagccgatgt tgatcgacat atagaacagg gtgaatgcgc catcaagccg
    11161 cggatctttc ggcggatagc acttcgaaag caagctggct gggttagctt taaacaggcc
    11221 gttaccgaca gcgatagtcc ccagggcgat gaaaatcagg tcaggcttaa gtagcgacat
    11281 gccggtcatg aagtagccaa tcgccagcac aagtgctcca agaacaatgg tgcgtttggt
    11341 ccccagcagg tggtcgccga catagccgcc aatggaaatg aggccataga ccagcgcagc
    11401 aaaagcacca aaagtgacaa aagcctgctc ttgcgagaat ccaagctgtt taacgaagaa
    11461 aaccgccagt acgccctgca cgccgtagta gccgaatcgc tcccataact cgacaaaaaa
    11521 gatcatgaaa aatgggcgag gttgctgcag catccccatg ggtgttgttg tattcatatt
    11581 tattaacctt ccaataccat cctgaaaagt atgaacgcgt aagggataac gcgcgaaaaa
    11641 gtttacatct gtcagagatt cttaatattg atgcggtgaa tagattaata cactattaca
    11701 acagaaaata accagatgat cacactaatg atggaaaata tgatggtgtt aaatgttgta
    11761 ttggctgggg attttgattg cgtacgcaga ccgtaggcca gataaggtgt ttacgctgat
    11821 caggtaacaa ggcgcgccaa aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt
    11881 cgtagaggga agattattct tcttcgtcgc gcagcggaac aatcagcata tcaacgtgaa
    11941 cggtgttgat cagctgacgt gcggaagaca tcagtttgct ccagaagtcc tggtggtgac
    12001 cacaaaccac caaatccata tcgtatttct tgattgcatc gaccagaacc tggcccaggt
    12061 cgccgctgcc gctcagggtt tcagtgattg ggtagcctgc attagtggaa agctcggtca
    12121 gtgcatgatg tgtctcttca gagatgcgtt tctgcatatc acccagattc acatcaataa
    12181 gcccagtgta taggtcagag tagtttacat ctacgtggat cagagaaact ttcgcattgt
    12241 aggggcgagc catagagact gctttctcta ccagaacttt gctttccggg gagaggtcga
    12301 ccgcgatgag aatgtgttta taagccatag tgttactcct tccataaagt tgtcgatgac
    12361 tggccagcta gcgtttcttg tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg
    12421 ggctaaccca gccgtaaaga ttcagtgtta agaccatcct taccttatag cgacccggat
    12481 gatccgtcaa tccgccttgc ttactcgtta gataaacaaa ttttcgacat gaatatattg
    12541 atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct atagagccgc
    12601 tcgtatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact gattggcagc
    12661 gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg gaggaggtat
    12721 gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg
    12781 ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc cattgctcta
    12841 tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca aacaggtgcg
    12901 tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg aatttattcg
    12961 ccgctgtgag cgggtgcgtc ggcagtttat tctgaccagc gcattgtgtg gtctggtggt
    13021 ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat aacgcataaa
    13081 aaaagcgggc cagtaaactg acccgctcga gactgattac aaacgcaaaa ttgcctgatg
    13141 cgctacgctt atcaggccta cataatctct gcaatctatt gaatttgcat gtttttgtag
    13201 gccggataag gcgttcacgc cgcatccggc atgaacaaag cgcactatgt cacaatctga
    13261 agcgggccag ctgacccgct cgttttggtg cgtacgatta caggaactgc aaggagagcc
    13321 agtacagccc gccggaaagc agtaccgcag ccggaagggt aaacacccag gccatcagaa
    13381 tgctggtcac ggttttacgc tgtaagccgc caccatctac caccatcgtc cccgcgacag
    13441 aagaggagag tacgtgagta gtggaaaccg gcatcccggt ataactcgcc aggccgatag
    13501 acactgccgc cgtcatctgg gcagacatcc cctgagcgta ggtcatgcct ttcttaccga
    13561 ttttctcacc gatagtcgtt gccacacggc gccagccaat catcgtaccg atacctaacg
    13621 ccagcgcgac cgccatgatg atccacaccg gtgcatactc gatggtgcta agcatgtcgg
    13681 acttcagttt cttcaacagg cgctgatcgt cagcactcac gccaggcatc ttcaccactt
    13741 tgtcgatagt gtcagaaacg cacagcataa tgcggcgcat ctggctacgt tgatcaagcg
    13801 acagcttgtc gtagctttcc acatcggtgg tcaacatacc tttcaggcgg ttgagcgcgt
    13861 taatggtatt cgacggatgg cagtggaact ccgcaggttg cgttgcgcca gcttccggag
    13921 ccggtactaa ctgatcagca ccggtagcct gtttgagcag cgcaggatgc tgctcaaagt
    13981 aagcttcgac gttgttgatg gcatcacggg tacgggtgat ttcgtagcca gtggcattca
    14041 tgttcaccac gaagcctgct ggcgcgacgc caatcaatac caacataacc agaccaatgc
    14101 ctttctgacc atcgttcgcg ccgtgcgaaa acgccacgcc gatagcggaa aggatcagcg
    14161 caatacgcgt ccagaacggc ggctttttct tgccgtcttt cttttcacgc tccgctgggg
    14221 tcaggtggat acgggcgcgt ttcttggtgc cgctccagta gcgacgcagc aagaaaatca
    14281 gaccgccagc aaacaccagg ccgacaatag gggaaacgat cagagaaccg aaaatactta
    14341 atactttcgg gatattgagt gcatccacca ctgacgtccc ggtcatcaac gcattggtta
    14401 aaccaatccc gatgatcgcg ccaatcagcg tatgagagct ggatgcaggt aaaccaaagt
    14461 accaggtacc caggttccag ataatcgccg ccagcaacat agagaacacc atggcaaggc
    14521 catgagacga tcccatatta agcagcagat ccgtcggcag catatgcaca atggcatagg
    14581 caacactcag accacccagc aaaacaccca aaaagttgaa taccgccgcc ataaccacgg
    14641 cgagctgaga acgcatcgcg cgggtataga taacggttgc cacggcgttg gctgtgtcat
    14701 ggaaaccatt gatggcttcg tagaacagca caaaagccag tgcaagcaat aataacagcc
    14761 cggtatgcaa atccaggcca gcaaacaaat gtagcatagg acgttacgcc attttgagga
    14821 catgaacgcg gcgcattatc agtgactttc gcggcgcggg caaagtgaaa tatagacttt
    14881 ttttgatttg ccccctgtat ggatttcact caaaaaataa ttatcttata taattcaggc
    14941 aaatacttcc ttttagtaat attgatgctg gtgcgaccac tgaggaatct ttacaattca
    15001 cgcccgtttt ttctaagagg agcgcaacgt ggaaaggttt gatgccatta ttataggcgc
    15061 tggtgcggcg ggtatgttct gttctgcgct ggcaggtcag gcaggacgcc gggttctgct
    15121 gatcgataat ggtaaaaaac cagggcgcaa aatccttatg tctggcggtg ggcgctgcaa
    15181 ctttaccaac ctttatgtcg aaccaggcgc ttatctgagc cagaatccgc atttttgtaa
    15241 gtctgcactc gcacgtttta cccagtggga tttcattgat ctggtcaata aacacggcat
    15301 cgcctggcac gagaaaacgt tagggcaact cttctgcgat gactccgcgc agcagattgt
    15361 cgacatgctg gtggatgagt gcgagaaggg caatgtgacc ttcagattgc gtagcgaagt
    15421 gctgagtgtg gcgaaggatg aaacaggctt cacgcttgat ctgaacggca tgactgtcgg
    15481 ttgcgaaaag ctggtcatcg cgactggtgg gctgtcaatg ccggggctgg gcgcgtcgcc
    15541 gtttggttat aagattgccg aacaatttgg cctcaacgtg ctgccgaccc gcgcgggtct
    15601 ggtgccattc actctgcata aaccgttgct cgaagagtta caggtgctgg cgggcgtggc
    15661 ggtgccttcc gtgattaccg ctgaaaacgg caccgttttc cgtgagaact tactcttcac
    15721 ccaccgcggc ttgtctggac cggcggtgtt gcagatttca agctactggc aaccggggga
    15781 atttgtcagc atcaatctgc taccggatgt ggacctcgaa accttcctga atgagcagcg
    15841 taacgcacat ccgaatcaaa gcctgaaaaa cacactggcg gttcatctac cgaagcggtt
    15901 ggttgaacgc ttacagcaac tcgggcaaat cccggatgtt tcgctaaaac agctcaacgt
    15961 gcgtgaccaa caggcactga ttagcacatt gaccgactgg cgcgtacaac ccaacggcac
    16021 tgaaggctat cgcactgccg aagtgacgct cggcggcgtg gacaccaacg aactctcttc
    16081 acggacgatg gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag tgatggacgt
    16141 caccggctgg ctggggggct ataacttcca gtgggcgtgg tcgagtgcgt gggcttgtgc
    16201 gcaggatttg attgctgtcc gaggacaata aagttgtaca caaagcacta aagttgtaac
    16261 cattctcaaa aaacgacgct aatgttgtat acttactgaa tgtgtggtta tagggttgga
    16321 gactataaaa tgcacatata ggtacgtatc aatgtcgttt ttagggtacg atcatggcta
    16381 ggggtaggcg tctcaaatcc tatttggatt atgaaaatgc gctaggtgac ggcataggag
    16441 tgggctatgg ccaaagttat cagccctggc ttagagctca ggacgttaaa tcccgtggaa
    16501 accgttcgat agtctttggc cttaagacgt ttcgaaacca tcatctcctt tcttctgtcg
    16561 aaagtaactt tttctatctg gctgagttta atgactcggt gattgatatc cgggaacaat
    16621 tcccactctt tcctctccgg cttacccaac aaatagcaaa tcatctacat tttcaacatc
    16681 ctatggtgag gggagtaaga ggagtacctg tcgaagttct gaatgttatg acaaccgatt
    16741 ttttactgac cttgagaact cctgaaggcg gacttcgata caaagctata gcagtaaaac
    16801 ataacgagag catacctgaa cgcgaagccc aaaaacttga aatagagagg atgttttggc
    16861 agttgattga tgttgagttt caaatttatg ttggctcgga actcaataac gtcgtcggta
    16921 aaaacatttg ctgggctact tctgtattaa gagatggttc tgaattttat gataaatatc
    16981 ctcttgataa aatcctatgg aagcttaaac cagatgttta tcccatagta ggactacgtg
    17041 caatgatttc atcaatcttt ggggtagatg cacaagaagc gatgatgtta ttgcaggcaa
    17101 tgattggatt aaaaatgata aatgttgatt tatcatatcc aatactcgaa accggtctga
    17161 taaagataat ttccaatgac cactatatag gactgaacgc aaatggatat tattaggaat
    17221 tcagtctggc tctctcaagg cactgatttg cttgcagagg gactttaccg tgttttggat
    17281 tttgacagaa aggtcgattt gttaattttg tttaaaataa aatcagaaag aacgggtaag
    17341 ccaatccctt tctcattttc aatgtttaaa tatta
//
LOCUS       DAZFZC010000098        16673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFZC010000098 DAZFZC010000000
VERSION     DAZFZC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.099"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(145..681)
                     /gene="yghD"
                     /locus_tag="ACHKXP_003900"
     CDS             complement(145..681)
                     /gene="yghD"
                     /locus_tag="ACHKXP_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO7648817.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIADRGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(683..1861)
                     /gene="gspL"
                     /locus_tag="ACHKXP_003901"
     CDS             complement(683..1861)
                     /gene="gspL"
                     /locus_tag="ACHKXP_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7648818.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMACDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1858..2835)
                     /gene="gspK"
                     /locus_tag="ACHKXP_003902"
     CDS             complement(1858..2835)
                     /gene="gspK"
                     /locus_tag="ACHKXP_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7648819.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRCVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2832..3437)
                     /gene="gspJ"
                     /locus_tag="ACHKXP_003903"
     CDS             complement(2832..3437)
                     /gene="gspJ"
                     /locus_tag="ACHKXP_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7648820.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRMPRSNLLTVGYRIHDGYLERLSWPLTDAAGSVKPTMQKLIPADSLHLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(3434..3805)
                     /gene="gspI"
                     /locus_tag="ACHKXP_003904"
     CDS             complement(3434..3805)
                     /gene="gspI"
                     /locus_tag="ACHKXP_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7648821.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTRGQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3802..4365)
                     /gene="gspH"
                     /locus_tag="ACHKXP_003905"
     CDS             complement(3802..4365)
                     /gene="gspH"
                     /locus_tag="ACHKXP_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7648822.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASSGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(4369..4824)
                     /gene="gspG"
                     /locus_tag="ACHKXP_003906"
     CDS             complement(4369..4824)
                     /gene="gspG"
                     /locus_tag="ACHKXP_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7648823.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4841..6064)
                     /gene="gspF"
                     /locus_tag="ACHKXP_003907"
     CDS             complement(4841..6064)
                     /gene="gspF"
                     /locus_tag="ACHKXP_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7648824.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPVSTSTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVIGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6064..7557)
                     /gene="gspE"
                     /locus_tag="ACHKXP_003908"
     CDS             complement(6064..7557)
                     /gene="gspE"
                     /locus_tag="ACHKXP_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7648825.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7557..9617)
                     /gene="gspD"
                     /locus_tag="ACHKXP_003909"
     CDS             complement(7557..9617)
                     /gene="gspD"
                     /locus_tag="ACHKXP_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO7648826.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9647..10606)
                     /gene="gspC"
                     /locus_tag="ACHKXP_003910"
     CDS             complement(9647..10606)
                     /gene="gspC"
                     /locus_tag="ACHKXP_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7648827.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYMAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTRQKAISDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQHDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10624..11034)
                     /gene="gspS2"
                     /locus_tag="ACHKXP_003911"
     CDS             complement(10624..11034)
                     /gene="gspS2"
                     /locus_tag="ACHKXP_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO7648828.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTLKMTIISEAGTQTTQTPDAFLTSYQRQMCTDPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11100..11909)
                     /gene="pppA"
                     /locus_tag="ACHKXP_003912"
     CDS             complement(11100..11909)
                     /gene="pppA"
                     /locus_tag="ACHKXP_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO7648829.1"
                     /translation="MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12107..>16673)
                     /gene="sslE"
                     /locus_tag="ACHKXP_003913"
     CDS             complement(12107..>16673)
                     /gene="sslE"
                     /locus_tag="ACHKXP_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO7648830.1"
                     /translation="NKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSL
                     PEVKPDPTPNPEPTPEPTPEPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVT
                     GDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQ
                     ELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKK
                     LVNEEVENNAATDKAPSTHTSPVVPATTPGTKPDLNASFVSANAEQFYQYQPSEIILS
                     EGRLVDSQGDGVVGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKST
                     IALTELGDEVRGANIDQLIHRYSKAGQNHTRVVPDEVRKVFAEYPNVINEIINLSLSN
                     GATLGEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQI
                     QGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDK
                     NYWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIG
                     NPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWKPDAKAS
                     MTVGTNLDTVYFKRHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGF
                     EYVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESAS
                     GFVRLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDS
                     KTGEVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKEK
                     IFAAFPGLKECTNPAYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQA
                     ADLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDE
                     LGFKTFTEFLNCYANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYM
                     EKPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTG
                     LWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVK
                     FKVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFV
                     YTTPKKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDSEDGKHRMFTYKNLPGHKHR
                     FTNDVQISIGDAHSGYPVMNSSFSPNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGA
                     TEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLK
                     EWAEKNFDIKKWYPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDEVSNDKFGGKNYC
                     AESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNT
                     LASLDLPKPEQGPETINQVTEHKMSAE"
BASE COUNT         3669 a         4773 c         4232 g         3999 t
ORIGIN      
        1 ttcttaagaa cccgcccaca aggcgggttt ttgcttttgg atctgacaat aaccttcacg
       61 aaaaaagtta gcttataaag tctgggggaa ttactctcgc cacgttaacg agagtaattt
      121 tattgatact aatctcctga tactttaccc ccgtccaaac tccagccgct gcacattcac
      181 catcccagtc ttctcagcag cactggcatc aatttgtgtc acccgcagcg catatttttc
      241 atccagtgcg tttaaccatt tcagcaggtc attaaacacc acaggttcta tccagacctg
      301 aatattctcc ccacgatcgg ctatccgcct gatgaccacc gagtgcgcgg aagcactgtc
      361 actgatgacc cgcgatacct gcgcaggcgt tgttgtggca gattttcgcg ctgcaataat
      421 atccggcgcg gcgttcttca gtcgggtgtt catcgccacc agctgctgca atattgtctc
      481 ctgttgctca atccgttcgc tcaacggctg ccagatgagg acgtaatatc cgacgctaaa
      541 caggaacacc accgctgcca gtaacatgcc cttttcacgc ggagaacgcc ccgccaggtg
      601 ttgtgccagc cagtgttcgc cacggcttaa ctggcgttca cgccattgct gaaaatagtg
      661 aataaattta tcgcgtaaca tgttatttcc tccgcaacgt tacgccgccg gaaaccgcat
      721 caccctcttt ctgtaacgcg tcctgttgca caacataatc ggttgccagt gcactacgca
      781 gtttgtcgaa actggcaaag ttcgcggccc gtagctggag gtgaagtatc tggcgttttt
      841 gatcaaaggt aaaaccacgc atttcgatgt cggaaagtga cgctgatttc agggtgctgg
      901 ctatcgctga caactcggcg agcagccggg tatcgtcggc ctgtgggcga tattttttca
      961 gcgccatcgt cacctgagag cgtaaattca caatccgctt ctgctccggg aacaacgtta
     1021 agaactgttc ctccgcctgg gtgcggcttt gcgccacctg ttcgctgacg ctccataacg
     1081 tcacgccccg ctccactgcc agcgcaacca gaatcagcaa tatcggcaga atcatcaccc
     1141 gccagcgcgc ccactgtttt cggtagctga cgcgaggctg ccacggccct gtcagcaggt
     1201 tcccttccgg ttcgccataa gtggtaatgg cgggcagagc tgtaacggtc aggcgttcag
     1261 cgtctgcgcc agcccatgct gatagctttt ccggtgcaat gccgactacg gttagcgaaa
     1321 gcggtaaatc ctgctcattg agctgggcgc ggaacatgac cggagccagc gccagcccgg
     1381 cgctccatcc ccggcattca tcgatgcggc agataacccg ttgcgcatcg caagccataa
     1441 acccacaagg aatggacatc cagtccggcg cgacggtagc gcgggtgatg ccgttttcct
     1501 gcaaccactg cgcaatgttg cgcatatgct gttggtgaat cactgctacg gttgccagtt
     1561 gctggtcgat attcaacggg gcgaaatgca gttcatcgat atcctggttc agctcttctt
     1621 ccagtaaggc gggcagaatg gtcggtatct gcttacgggc gacatcaggc agttcaacct
     1681 gccagacgct gatccattcg ccgggaatgt agagtcgaat cgcatcggtt tgcagccatt
     1741 gctggagctg ttcgttagca acgtcagacc agataccgtg ctccacatcg ggtgtacggc
     1801 gctgccaatg gatgggatca gcggcgcaaa gcgggaaaaa aatctcaagg atggaactca
     1861 ctcactttct cctgtctgat gccagagaac cgaaaagtgt tgtgggccca tgcggacaat
     1921 taacgagttc atcgtcagtt caatctcatt cacggtgata tctgaacgca gccagaagta
     1981 attgctgtcc acgctcagga cggtttttag ctgtttttta gtacgctcat cgacgtcagc
     2041 aagtagcggc tgtgcaagaa actgatcgac atcttcccag cccttcgccg gacgttgttg
     2101 taataacgcc cgcgcctgaa cagggcttaa ccacgggtca aacagcgcct caagaatcac
     2161 actttgcgtg acgtctaagg tattgatgtt gatttgctgg cgggtcatcg gcagcgcaca
     2221 gaccagcggt ttcagttttt gataaagccc ggcgtccatt ccctgcacca cgcgcatctc
     2281 gctgatatcg gccagcggtt gattggcggc gtagaacggc accgagcggg cgagatactc
     2341 gctgtcttca cggcccagac gcgtctgcac gcagcggtcc tcgtcaataa actcccacag
     2401 gctttcggct atcagttcgg cccgataagc aggcacatcg aggcgcgaga tcagggcaat
     2461 cagttgttgt accgcgagcg gacgtgacgc cgttgtgggc tgagcgaggg cattcaggtt
     2521 aaagcaggcc tgtgcgtcac gcagagtgac ggcgatttgc ccctgcggca gtgggaaaaa
     2581 acgagggcca gaagcccacg gttgtgccag atgcacgcgc ttttcatttt tcaggctcag
     2641 actgagtgca ctcagcgcca ggctttctgc actggcgctg taccacagcg cctgctggta
     2701 ctcctgctgg ctgcgcgttc gcccaagttg ctgctgcatc cgcccggaaa gcgtgatggt
     2761 taccagcatc ataactgcca gcaataccag caccacgacc agtgccattc cgcgttttgg
     2821 tggtgaggtg atcatgataa ttgcggcccg cgtaacaacc agatgcgttc aatctcgccc
     2881 cattgcggcg aatgcagagt catgcgcact gccacaggga tcgcctgtac tgacgaccag
     2941 ctctcctgcc agcgcgtgcc gtcgtagaac tgcaaatgga gcgaatcggc cggaatcaac
     3001 ttttgcattg tcggcttcac gctgcctgcg gcatcggtca gcggccagga caaccgttcg
     3061 agataaccgt catgaatgcg gtaaccgacg gtgagcagat tgctgcgtgg catacgcatc
     3121 aacgggttaa ccacaccgcc gcgcacaaaa cgcattcctt cactctcaga cgccagcacg
     3181 ccagcgccag ccagtaaagc tggttcacgc tggccctgat cgcctcttac cggacgcggc
     3241 atcatttggg tcagatcgtg ggtcagaaaa ctcatcgttt gctgcatgag gtttagtttt
     3301 tgatcgtgat cggcgacggc gctattcacg cgcgtgacgc cgttagtcac ctgctgcgcc
     3361 atcagtgcca gcgaggcaaa aatggcaatt gccaccaaca tttccagtaa cgtgaaacca
     3421 gcgcgagtcc ttctcactgt tggcctccca cggcgctaaa ccaggcgcgt cgtgactgaa
     3481 tgaccgacga aaagtcttcg tgaaggctga cttcaatatc caccgcctgg agcagcgcgt
     3541 tgcctgtact cagcggtgtg gttcgccagt accagcggct tcctgccata tcgctctcgc
     3601 cctgctgcct gaccgcccgc tgcccacggg tcatcaggta gagcagcgca gtctggtttt
     3661 cagccaccca gcccgctacc gttttttcct caagaacgtg ctgattactc agcgcaccgc
     3721 tggcaatctg taataccgcc gtggcagcca gcgcaaaaat cgccagcgcg agcatcactt
     3781 ccagcaaggt aaatccgcgc ttcatggcat cctctcatca cattgattga gggataacgc
     3841 gccatcgtgc gccagtttta ccgcccaaca tgcgttttgc gccgccgagt agaagcgcag
     3901 cgtaaacggc gtggcgggtt caaaaggcga aaaacggatc tgcggcgtct tctttttcgc
     3961 ctctttttgc agctccagtt caatatcgtg cagcgtcagg cgacgacgtt gcagctccag
     4021 cgcatactcc ttctgccaga tatcacttcc gggttgtaac agcatctgca cctgttttgg
     4081 caccgtaacc tgtgccgata accgggtcgc ggaaacgggc aaccactgcc cctgacgacg
     4141 ctgcataaac tgatagccag gcgcgtcgat tagcacaccg agtgtctgcc cttcaatcac
     4201 tgccctgtcc ttaaactgcg caaagcgagt cagaaaatcc tgtgccgctt ttttcgcggg
     4261 cggttcagaa tcggtcgcaa acgtctgtac cacgcccgaa ctggcaaggc cgataaggaa
     4321 aatcaccaac atgatttcca gaagtgtgaa tccgcgttca ggcactgctt actgaaactc
     4381 ctgcaaattc cagttaccga tatctgcgcc agcgccttcc ccgttttcct gaccatccgc
     4441 ccccagggta taaacatcaa acagcccttt ttcacccggg ctgagatact gataatcatt
     4501 gccccacgga tcctttggca gtcgtttaat gtatccacca gtacggtagt tgcgggcgtc
     4561 cgccatattg gctggctgct ggatcagcgc ctcaagcccc tgttcggtag ttggataacg
     4621 cccgttatcc agtcggtaca tatccagtgc gttttccagc gccacgatat cgctgatggc
     4681 tttttgccga tccgctttct ctttattgcc caacaggtta ggcaccacca gacttgccag
     4741 gacgccaaga ataacaatca ccaccatcac ttccagcagg gtaaaacctg cccgtggttt
     4801 ttgtgtgcgg gataacgaat tcatgtgata actccgtaaa ttacattcca accatattgt
     4861 tcagttgcag catcggctcg aggatggcga tgacgataaa cagcaccacg cccgccatca
     4921 tcaccaccag cgccggctca aacagcccca acgccagccc cacctgggta tcaaattccc
     4981 gctcctggtt aactgcggcc tgttcaagca tggtttccag ttcgccgctc tgttcgccgg
     5041 aggcgatcat gtacagcatc atcgggggga acaggcgcaa ctccgccagc gcagcgcgca
     5101 gactgcttcc ttcgcggacg cgatctgccg ccaacagcag ttgttgctcg acatagcgat
     5161 ttgccgacac ggcggcagcg gtctgaatgc cttccagtag cggaacactg ctggcggtga
     5221 ggatgcttaa cgtgcgggaa aaacgcgccg tattcagtcc gcgcgcaaca cgccctatca
     5281 cgggcaggcg cagcaaggtt ttatcccagc gcaggcgcat cgcaggattt ttaagtagcc
     5341 gttgccccag caccagaagc cccagcaaac ccgccagcca gtaaacgccg ctggcctgca
     5401 acgcatcgct catggcaata agcgtgctgg tggagacggg cagcgcgtgt ccgagatgat
     5461 caaactgttc gatgattttc ggtactaccg ccgtcagcaa aatagtgacc acgcccgttg
     5521 ccaccaccag cagaaccagc ggatagagca tggcctgcaa tagacgtgat ttcaggcgct
     5581 gtcgctgttc ggtgtaatct gccaggcgat taagcaccac gtcgagatgc ccggattttt
     5641 ctccggcagc aaccatcgaa caaaacagag aatcaaagac gcggggatgt tcgcgcaggc
     5701 tgtccgacag ggtgtaacct tcctgaatcc ggctgcgcag cgccattccg aggcttttta
     5761 catgcagttt ttcactttgc tcactgaccg cctgtaagca ggtttccagc ggcattgctg
     5821 cctgtaccag cgttgccagt tggcgcgtga acagcgcaag atctgccgcc gccacgcgac
     5881 gatgtgcgtg ccgccgacgc tgcaacatcc cccctgacga agtattcatc cgggcttcaa
     5941 tatgcacggg gataagctct ttaccgcgca acaactggcg ggcatgacgc gcggaatccg
     6001 cctcaatcat acctttggtt ttgcgaccat tacgctccag cgcctgatag taaaacagtg
     6061 ccattacgcc tccatggtta cccgcagaac ttcatcgaga gaggtttctc cggcgagcac
     6121 tttctcaatg ccgttgctgc ggatacccgc agagtgttgt cggacataac gttccagctc
     6181 cagctccccg gcctgacggt ggatcaaatc acgcaatgtg gcatccacca cgatcagctc
     6241 atggatggca gtccgtccgc gaaaaccttt gtgattacag gcgggacagc cctgtggatg
     6301 gtacagagtg acggtacggg cgtcggtaat tcccagcagg cgtttttctt cgtcagtggc
     6361 tggtgcgggc tggcggcagt cgggacacag cgtgcgaacc agtcgctgcg ccatcacgcc
     6421 cgtcagactg gaagagagca ggaaaggctc cacgcccata tcctgcaaac gtgtgatcgc
     6481 ccccaccgct gtgttggtat gcagcgtgga aaggaccagg tgtccggtaa gtgaagcctg
     6541 gacggcgatt tctgcggttt cggtatcgcg gatttcaccg accatcacca catccgggtc
     6601 ctgacgcaaa atcgcgcgca gcccacgggc aaaggtcatg ccgacgcggg tgttaacctg
     6661 cgtctgaccg atcccttcaa tcatgtattc gatgggatct tcaaccgtga gaatgttgcg
     6721 cgaatggttg ttcagctcct gcaatccggc gtacagcgtg gtgcttttgc cggaacccgt
     6781 cggccccgtc accagaaaga tgccgtgcgg tttgtgtaac agctggcgca actgcgcggt
     6841 cagttgctgg ctaagcccca gacgctccag cgtcaggcgg gcctggtttt tgtccagcag
     6901 tcgcagcacc acgcgctcgc cccaggcaga aggcatggtg gagacacgca cgtcaatcgc
     6961 ccggccgccc agcaacagcg caatacgtcc atcctgcggc acgcgctttt cggcaatgtc
     7021 cagccgcgcc atcaccttga tacgcgacac cagcagcgag gccagtttgc gccccggacg
     7081 cagcatttca tgtaatgtgc cgtcaacacg aaaacggatc accagactct tttcaaacgt
     7141 ctcaatgtgg atatccgaag cgccttcttt gattgcctct gccagcatgg cgttgatcag
     7201 tttgatgatc ggcgcatcgt cgtcgctttc cagcagatct tccgtttccg gcagttcttc
     7261 cgcgagggta aaaaagtcct cggcagaacc gagatcttcc atcagctggc gagcctcgga
     7321 agagtcccgc tgccagaccg cattcagccg ctgttcaaat tcggcctcgt cgatttgccg
     7381 cagcgtaaag ggcgcgttca gcccccgctg cagctcctgc aaaacagaga gcgatagcgg
     7441 atgaacgtgg aggatctcca gcgacgcttc gcaccatgcc accaggctaa accgacggct
     7501 gaaactgtag ggcagacgca cggtgttggc ggtggtttcc tgtgctacag gcaccattaa
     7561 cgcgttctcc cggcattgag gaacgcgcga acttccggcg gtaaggcctg gttttgcgct
     7621 ggcagtaccg gctgtgcagt gtgcggcatc aggcttaaac cttgctcatc acgatagatc
     7681 tgttcggcgc gcatatagtt atatttgcgc tgcgacacgc cgtctgccgc cataccgtca
     7741 cgcagaatgg tcggacggat aaacaccatc aggttacgtt tttctttttt atccgccgtc
     7801 gatttaaaca ggttaccaat caatgggata tcgcccagca gcggcacttt cgccacgctt
     7861 tctcccgcct gatcgtccat cagaccacca agcacaatca gctcaccatc gttagccagc
     7921 acggtggttt tcagtttgcg ctcaccaaac accacgtcga ggctggtctg tccttccacc
     7981 ttcgagactt cctgctcaat caccatctgt accgcgttac cttcgttaat ctgcggcgtg
     8041 actttcagca tgatgccgac ttttttcctt tctactgtat tgaaaggatt gctgttattg
     8101 gagccaacgg tagagccggt taataccgga acgtcctggc ccaccatgaa gaaggcttcc
     8161 tggttgtcca gcgtggtgat gctcggcgtg gagagcacgt tagagctgga gtcgttttta
     8221 accgcctgca ccagcgccat ccagtcgcct ttcaccacac caaccgccgt accgctaaag
     8281 ccggaaagaa gctgggcgag cgtggagaga tcgccgttgg tatccggatt aatggtggta
     8341 gcgccgtttt cactgatcac cgtcgagcct ttctgcggtt tcgcctgaga aatggctgcg
     8401 cccagcgtac cgatagggat ctgcgtacca ttagcaaact gcattaatcc ggcatctttc
     8461 gacgcccact gcacgccgaa attgatattg ctaccttcgg caacttccac gatcaacgcc
     8521 tcgacatgca cctgagcacg gcgaatatcc agttgttcaa tcacgctttg cagcgattgc
     8581 atgatgtcct gcggcgcagt aacaatcagg gcattactgt gtttgctggc ggcgatggag
     8641 acaatctcac gcccgctacc gaccgtgcct tccgcctctt ctttagccgc cgtgagcgtg
     8701 ccgctgacct gcttcagtac atcgaccaga tcttcggctt tgctgtattt gagatagaaa
     8761 acctggctgt tgccgctgcg ctccatttct gagtccaggc gacggatcag acggcgcatt
     8821 ttgtcccgcg tggcggggtc accactgaca atcacactgt tggtgcgttc atcggcgaca
     8881 atttgagatt tcagcgtcgc aggctggttc tcgccgctgt ttttcgtcag gctttccagc
     8941 acgcgggcga tttccgaagc cgaggcgtta tccagcggga tcacctcttc agtgcgatta
     9001 cccgcgtgat ccacacgctg gatcacttcc gtcagccgct ccacgacgga ggcgcgtccg
     9061 gtgagcataa tcacgttgga gggatcgtaa ttaacaacgt tgcctgagcc tgcgctgtcg
     9121 atcatctggc gcagaatcgg tgccagctcg cgtactgaca cattacgtac cggcactact
     9181 ttggtgacca tttcatcgcc cgcgtagttg tcgctgcctt caccgaccag cggcagcggc
     9241 tcgactttcg cagcgctgga tttcaccacc ttcagcacgt cgttttccat cggtacgacg
     9301 gcatacccct gtgcttccag caggttaagg aatagctggt aatactggcg ttcattgagc
     9361 ggggtcatgg tgcgaatact cactttcccc tgtacgcccg gccccataat gatggtttta
     9421 ttaaggttag cgccgacggt ttcgatgaac gatttcaggt cggtatcttt aaaattagcg
     9481 gtgaaagtgg cttcttccgc ccagaccggt gaactgcata atgccgctgc cagcaccagc
     9541 ggcagtaaac gcttttttgt tttgaggcca gttgtcttct tacgccagac cgacaacgta
     9601 atatcacgcc aaaacacgat gaatgattct cctggattaa atgcggttag cgcagcgcga
     9661 tggaaatgtc gtggcgcgca cccttgcgta aaaccgtaag ttgaatggaa tccattgaag
     9721 gtaactgccg catcagagca atcattgctc gtggatcagt gaaatcatgc tgatttagcg
     9781 caatggcgat atcgccttcc ttgaaaccgc tggcatcgaa cagagaacga tctgcccccg
     9841 gcttcactgc ataaccgaca attccctcct tacgcacagg cgtaagctgg atatagttaa
     9901 aaattttctg cggatctttc gccagtgcct gacgcacggc agtcgggatc tcaactggcg
     9961 cactgacagc aggttcagca acagcttgct ttgcttcgtc actgatggct ttttggcggg
    10021 tcacagcaac cgtggaacgc tcctcttctg ccaggctcag gcgctctatt tttccctgat
    10081 agcgcagcat cacatggtcg cgattgattt cctcaatcac cgcgttgtga gaaccaagcc
    10141 gttccccctg caaatagacc tgctgtttgc caccttcttc aataaccgcg ccgggtctgg
    10201 caccaaaggc gatcccacgc aacaccacat taagacgcgt ttctgccaca ggtgcaggtt
    10261 cgggttgttt taccggcgtg gcgacgggct gatatttgcc aaaccagttt tgctggctga
    10321 ttaattgcac gtcattttta tcgaacgttt ttgcatccgc acggggcggt ttattcgccg
    10381 atggggaaac agccatatat tccgcagaaa aagagaaata gcgccagagt gaatgcgcca
    10441 cttttgcaga aataataagc agcatcagcc agaacatccc gcgtacaatt tttcgcagat
    10501 gctctttgtc tgtattaaga gattgtctct ggttaagagt gtgacgaatt tttgtcagcc
    10561 attgaatgag ataaattcgt gcgtcacgaa aaacaacccg cgccaattta tatctacccg
    10621 acgttatgct ttaactattc cacaggtagt gcgatccagt ttccgctgat actggttacc
    10681 tgtacgtgta tcattaatcg ttatgctgta attaattccc tcagtgagca ttaatttcac
    10741 cgttgggtca gtgcacattt gtcgttgata gctggttaaa aaggcgtcag gcgtctgcgt
    10801 ggtctgagtc cccgcttcgc tgataatggt catttttaac gtcgtgccgc tactttgtgc
    10861 cagcactaag gtatatcccg cagatttaat cggcaggttt tggctgatat tttgcgcctg
    10921 ttttttcgcc agtaaactgg cgttttcatt atggctggca cagccactta ataatcctgt
    10981 aacgctcaac aatagcgata ttaataccct ccctggcatc tgttttatcg acatggtaaa
    11041 taatctctaa ggttattaat aagagttaaa atgtcacttt gataatgacg tggttatcat
    11101 taaaacaatg cctgtagata aagtgttgct ataccgccca gacttaaaca cggtccaaaa
    11161 ggcagtgtgg ttgatcctct ttttgtaata acggcatata tcaggccgca gcatgaggcg
    11221 attaaagcaa cattgggcag cgacaatgcc cccacccagc cacctaacgc ggcgaaaagt
    11281 aatacatcgc ccatgcctaa tgcttcttta cgcagaacta ttccggctat ccaacgcaga
    11341 gagtaaaaag tgataaatcc caccaggacg ccggtgactg catcttgtag cgtgagcgga
    11401 ctctgctgcg cccatgccgc aatcagtcct gtccacaata cgccctgagt aaaaacatcg
    11461 ggcagccatt ggtgatcgag gtcgatgacg ctcgcggcaa tcagccaggc ggataatatc
    11521 atcaccgcca gcccccatcc actttctggc cagaccagac tcgccagcaa aaaagcaagt
    11581 gctgtcaata actccaccag cggataacgc ttgctgattt tcgcctgaca gtcgcggcag
    11641 cgccctttga gcatcagcca ggagagcagc ggaatattgt cacgaacgcg gatggtctgc
    11701 tgacaatgtg gacagtgcga acgtggcagc gccaggctta tttttgactg cgcactcggc
    11761 atttcaccgt gaaactccgc catttgttgg cgcagcatga tggggtaacg ccaaatcacc
    11821 acattcaaaa aactacctat aatcaatcct ccgacggttg ccaggacggg catcgccgca
    11881 gggtattgct gaaaaacatc aaaaagcatg gctaaaggtt atttgttgta acttgctgga
    11941 tgcggcgtaa aacgccttat ccgtcctacg ggtgtctgcc agcgcaaata atcgcggctt
    12001 tccccgtctg taggcccgat aagcaggcgc atcgggcaaa tgtgttaacc cggtgcgcct
    12061 tatttcatgc cggatgcggc gcgagcgcct tatccggcct acgggcttac tcggcagaca
    12121 tcttatgctc ggtaacctga ttaatggttt ccggtccctg ttccggtttc ggcagatcga
    12181 gtgacgcgag cgtgttgtaa gccgactggc tcacaccgcc ctcgaagctc atctcgctcg
    12241 cccccggcag ctggtaagca ttcgcgcccg gattccattt cttaaagaac tccgaaagat
    12301 ccgtctgggc gacccaggag gcacacagca tcagcgtgtc cgctgcgtta ccgttggatt
    12361 cagcacagta attcttgccg ccaaacttgt cattgctgac ctcatcgccg cgtgctttac
    12421 gatgcatcaa ctggaacagg ttccagcctt tcatcccttc acgctcgctg taaaactctg
    12481 gcagaggagt gccatctgga taccatttct tgatatcaaa gtttttctct gcccattcct
    12541 tcagctgtgc gtacatcagc agacggtcac ccgcaccgcc gcgtgcccat gcctggttgt
    12601 tgctctcctc cagatattcc ggtgcgacgg taatatcgtc agcgacacgg ttcatcttgc
    12661 cgagataacg atcctgcatg tacagcgcca gcacgttgtt agcgacttca gttgcacccg
    12721 gtacagtcaa cggcgtttct gcggcgttat gaccgacttc atgccagatc agccagtcgt
    12781 tcagcggcgt cgtcggcagc gtggtgctgt tcggcgagaa gctgctgttc atcaccggat
    12841 aaccagagtg cgcatcaccg atggagatct gcacatcgtt ggtgaaacga tgtttgtggc
    12901 ccggcaagtt tttataggta aacatccggt gcttaccgtc ttcgctatca cggccgtaga
    12961 agtcattcat cgagctggca aaggtatcca gatcgttagc gaattgctcc agtccgccag
    13021 tgtaattgct ggcattcagg ttcttcttcg gtgtggtata gacgaaagcg tctgattcca
    13081 gctcacccag cggagccggt gagttcagat cgtttttcca tgcgccgtct ttatagaacg
    13141 gtgcttttac cacgccagta aaggtgaagc tggcagattc attggtagag ctattgccct
    13201 tgatataaat caggccaccg taaggcacct tgaacttcac cgtaccgcta gcgtccagag
    13261 agtacgtttt agtcactctt ggcggacggt tcagcgcaac ttcatgcttc tcacgtccgg
    13321 tcaggtcgtc agccagcgcc acggtgacgg tcacaggaac gttcgcattg gacttaatgg
    13381 tgacctcttt ctgagccggt gcccacaggc cagttgactg catgttacct gcaaaccatt
    13441 tggtcggatt cgagtacagg ctgatggttt cagtaacgtt ctgtccctct tccgatactg
    13501 ctcccggata cttctcgaca tcaactttga tgttcagatc ccaccaggaa cggcccagca
    13561 tcaggcgtgt cagcggtttt tccatatagt tgagcgggta gctcgggttc atcatgcccg
    13621 ctttgctgct accgtcaccg tagatcatgt tgttatcgac cagcgatttt ttcagatctg
    13681 cagaacactt ggtgccgcct gcataggcat cattggcgta gcagttcagg aactcggtga
    13741 acgttttaaa gcccagctcg tcatttttgc cttcttcata acgatagctc gtatcgttcc
    13801 acagccagac cgacatgttc tggtacagac gttccagatc gacgctgctc agacgctcac
    13861 ctttgcgacc attggtccgg aagtagagct catgctgata cagacgctga atgttggtgc
    13921 ctaaatccgc agcctgcacc atcgcttttg cagtgtcggc gttaaggctt agttgcgtat
    13981 actgtggaac atacatgcca ccagtaaccg gaacccccgt gccaggacga tactccaggc
    14041 agttgacctc atagtgatat gccggattag tacactcttt cagccccggg aacgcggcga
    14101 aaattttctc cttcgcagcc ttcagagaat cctctgtttt atgatcggcc tcatcaataa
    14161 aggcataacg cgtttcctgt ttgccatcta catcttccag ccagctggca acttccagct
    14221 tcggtttgtc atcaggtttg ttttctacct gatatttcca cttaacttcc cctgtcttac
    14281 tatcgatggt gtacggcagc gcaccatcta cggcaggata acgttcatag acccaaatgc
    14341 ccgttgcgcg ctgctgacga acgcggttcg gatacccttg cggatcgtta tttactaccg
    14401 acttgttcag tgccatcgac agacctgcgg catccaacag acgcacaaaa ccagacgcgc
    14461 tctcttcctt aagattgctc atcacgtttt ccatgatcag caccgatcca cctttgttca
    14521 gataggcgat cagatcggtc acatcctgct gagtcagctt cggtttgctg gtatctgcac
    14581 gcagcgggat tgcataagga tcgttaccca cctgagtcac atattcaaag ccgttaagga
    14641 tcagcagcgg catttcctgc ggatcgagat cgccatagct acttaaatgc tcaacagaga
    14701 tgcccgcaaa atccggatgg aagtcgaacg cagcgctgtt tcctgtaacc tgaccatgac
    14761 gtttaaaata gacagtatcc aggttggtgc ctacggtcat gctggctttc gcgtccggct
    14821 tccatttatc gtcggacaga tagcgcagca cgttctccat gaagttcttc atgtcatccg
    14881 ggtcgctgtt gagcgtacac tgcccatctt tattaacgcc cccgttccag ctgtaaccgt
    14941 tcgggcaacg caaaatgctg ttgtagtgtg ggttaccgat aaccatcagt ttgccctcac
    15001 cgacttgccc cagcgaaata aacggcaggt taaaggtggc ggtatcgcgc gtaacgtttt
    15061 ccggctcaac aagagaaggc gcttccgtaa tgtacgccag ctcgttttta tcccaggcgc
    15121 gtttttcgcc gaaggccagc cagtagtttt tatcattacg cgccatcaga atcgggaagg
    15181 ccgcgttgga gatattcacc accgcctgac cgcgcgcatt acccgtgctg ccatagaagt
    15241 tggtggagtc atggaataca tggaacttac tgacagattt gtagttcgta tccacgcccc
    15301 acagcttgtt gataacgccc tgaatctggc cgtcattaac attgcgcgtc gtcagcgaga
    15361 accagcgagc ctcgttacaa ccgtcggttt tcgcacaaat cgcggtatcg atctctttgg
    15421 cctgacccgt cttaaactgc tcgataaatt cgttaggcag attaacgact tgctcacctt
    15481 cccccagcgt cgcaccattg gataacgaga gattgataat ctcgttaatc acgttgggat
    15541 attcagcaaa aaccttgcgt acttcatccg gaactacacg cgtgtgattt tgtccagcct
    15601 tcgaatagcg gtgaatcaac tgatcgatat ttgccccgcg aacttcatca cccagttcag
    15661 tcaatgcaat agtcgacttg ttaccacgca cagaaccaag ctcaaaagtg tcgatgccaa
    15721 aggagatggt ttccccccaa ctaaaggaaa attccccgtt ttctccggtt acaccacggc
    15781 cggaattggt gtaatagttg acaccaacaa caccatcccc ctgactatcg accaggcgac
    15841 cttctgagag aatgatttcc gaaggttgat actgataaaa ctgttccgcg ttagccgaca
    15901 cgaaggaagc gtttagatcc ggttttgttc ccggagtggt ggcgggcacg accggtgaag
    15961 tatgagtgga tggcgcttta tcggtcgcgg cattgttttc cacctcttca ttgaccagct
    16021 ttttgaactc ttccggtgcc agatcgattt gcttatacag cgagtcgaaa cgtttactct
    16081 cgatcaccga ggagaactcc aggcacacct gttctgtatt cgccgggcaa ctgttcatgg
    16141 aggtgaccag cgagagcgca ttgcttttct tgttgtcgga acccgccagt tcttgcgcgt
    16201 cctcaagact aaacgacact ttttcaaccg cacgcaggct acgcgcagct tctgactggg
    16261 tgtcgaaggt agcaattgtc gtgttgttcc ctgccacaca ggtgactttg tcgcctggtg
    16321 taaaggtaaa gccatcgctg gattcatcat tacaggtgat atcaccagtt acccgcaggc
    16381 ttccgcccag ggtcagataa cccgttttcg taggaacagg ttctggctcc ggttctggtg
    16441 ttggctcagg atcaggtgtc ggttccggcg taggttcagg ctccggcgtt ggctcaggcg
    16501 tcggctccgg gtttggtgtt ggatcaggtt tcacttccgg caaagaccct gttccagaat
    16561 ctacaggcgg cgtatcggag gaagatccgg aaccaccgcc atcacaaccg gctaacaggg
    16621 ttgcgctcaa aatagccgct aaaagcgatt tcttatattt aaatttctta ttc
//
LOCUS       DAZFZC010000099        16606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFZC010000099 DAZFZC010000000
VERSION     DAZFZC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.100"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(88..765)
                     /gene="narI"
                     /locus_tag="ACHKXP_003914"
     CDS             complement(88..765)
                     /gene="narI"
                     /locus_tag="ACHKXP_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7648831.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(765..1475)
                     /gene="narJ"
                     /locus_tag="ACHKXP_003915"
     CDS             complement(765..1475)
                     /gene="narJ"
                     /locus_tag="ACHKXP_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7648832.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1472..3010)
                     /gene="narH"
                     /locus_tag="ACHKXP_003916"
     CDS             complement(1472..3010)
                     /gene="narH"
                     /locus_tag="ACHKXP_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7648833.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3007..6750)
                     /gene="narG"
                     /locus_tag="ACHKXP_003917"
     CDS             complement(3007..6750)
                     /gene="narG"
                     /locus_tag="ACHKXP_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO7648834.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKEKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7266..8657)
                     /gene="narK"
                     /locus_tag="ACHKXP_003918"
     CDS             complement(7266..8657)
                     /gene="narK"
                     /locus_tag="ACHKXP_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO7648835.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            8996..10792
                     /gene="narX"
                     /locus_tag="ACHKXP_003919"
     CDS             8996..10792
                     /gene="narX"
                     /locus_tag="ACHKXP_003919"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO7648836.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10785..11435
                     /gene="narL"
                     /locus_tag="ACHKXP_003920"
     CDS             10785..11435
                     /gene="narL"
                     /locus_tag="ACHKXP_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO7648837.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11436..12830)
                     /gene="ychO"
                     /locus_tag="ACHKXP_003921"
                     /gene_synonym="iatA"
     CDS             complement(11436..12830)
                     /gene="ychO"
                     /locus_tag="ACHKXP_003921"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO7648838.1"
                     /translation="MSRFVPRIISFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMVRGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13016..13369
                     /gene="ychN"
                     /locus_tag="ACHKXP_003922"
     CDS             13016..13369
                     /gene="ychN"
                     /locus_tag="ACHKXP_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO7648839.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13413..14108)
                     /gene="chaC"
                     /locus_tag="ACHKXP_003923"
     CDS             complement(13413..14108)
                     /gene="chaC"
                     /locus_tag="ACHKXP_003923"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO7648840.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14266..14496)
                     /gene="chaB"
                     /locus_tag="ACHKXP_003924"
     CDS             complement(14266..14496)
                     /gene="chaB"
                     /locus_tag="ACHKXP_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO7648841.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14766..15866
                     /gene="chaA"
                     /locus_tag="ACHKXP_003925"
     CDS             14766..15866
                     /gene="chaA"
                     /locus_tag="ACHKXP_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO7648842.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16271..16378
                     /locus_tag="ACHKXP_003926"
     CDS             16271..16378
                     /locus_tag="ACHKXP_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7648843.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(16392..>16606)
                     /locus_tag="ACHKXP_003927"
     CDS             complement(16392..>16606)
                     /locus_tag="ACHKXP_003927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648844.1"
                     /translation="SIHLHFIPEMFAIKSTFRLFNSCCSRFMSALDDASSSGCQYLLV
                     EEIKTKMAAGCYPCGRFRAHIAITAA"
BASE COUNT         4034 a         4427 c         4162 g         3983 t
ORIGIN      
        1 ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa ccccgccgaa
       61 gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct ggtacttacg
      121 tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg ggaacagcag
      181 gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac catcgaggtg
      241 ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca gtttcatcat
      301 ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca gcgcgcactg
      361 gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac gcacgcgtgg
      421 gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca cgccgctggc
      481 accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg cttcatacat
      541 ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa taccgatatg
      601 gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg acgccgcgcg
      661 ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga ccgccccggc
      721 tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg ctgtcctccg
      781 gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg atgagcagtg
      841 attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc ttcccagaca
      901 gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc cgccactttg
      961 tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac ggcataacgg
     1021 ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat atctttcaaa
     1081 ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag cggcagatgg
     1141 tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc cagcaggtcc
     1201 accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag caacagtgac
     1261 gtggcgcggc cacggtcgaa cagttcgctg tactgggcct gggcatcgag cggatccatc
     1321 gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga cagatttttc
     1381 gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc atccggatat
     1441 tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt ttgctggtca
     1501 catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct gaaccgtggc
     1561 agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc cgtgccagtt
     1621 cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc agataacggt
     1681 acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg gtatcaactt
     1741 taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt ttcagtgcgc
     1801 gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt accggaatac
     1861 gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg tctgctgcag
     1921 actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga tattctggat
     1981 gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac tgctgtgcgg
     2041 cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact ttcggatcgt
     2101 tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc tcggtgctgg
     2161 ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg atacgaccga
     2221 cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag cagaagatgc
     2281 acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg catccggtga
     2341 tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca tcttcttcac
     2401 gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg cagtgttcgc
     2461 acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc gccttctgga
     2521 tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg tcatcttccc
     2581 agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa cgcggacggg
     2641 caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgataa tcaaaatcga
     2701 acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt ttacccagca
     2761 gcatggcgcg gttacccatg cgcggctgca gtttgccatt gattttacgg atccagccgc
     2821 ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc ttggtttcca
     2881 cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt ttacaggtaa
     2941 ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg acttgtgaac
     3001 gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta accagtcaat
     3061 gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg tgccgtagta
     3121 gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt tcggcgtgat
     3181 acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac caggcaggtt
     3241 aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac gctggctgac
     3301 aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt cgttatcggc
     3361 gatacccaga tctttggcat cggcttcact caaccagacc accggaccac cgcgacctaa
     3421 agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct ggtgcggcgt
     3481 caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca cttctttcac
     3541 cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat cacgcatcca
     3601 ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga tcagctcgtg
     3661 aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag accaggtcgg
     3721 gctggagata attttgcgcg gctgtgcctg aatatcgcgg aagcggatct tctcgtcttc
     3781 tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg cagcccaggc
     3841 tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct ctgccgcatc
     3901 aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg tgtagttgag
     3961 cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac cgccgttacc
     4021 gattttctcc atcagcgggc cgatagaagt aaagcgttcg taagttgccg gataatcgcg
     4081 ctctacgacc ataatgtgcg gtgcagtttt gcctgggatc aggtcgcatt cgcctttttt
     4141 ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct ggataggcag
     4201 cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga atttcttcgc
     4261 gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga ccgccgcaga
     4321 cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt cgtaccaggt
     4381 cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga agtccagcgt
     4441 aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt cttccggctt
     4501 cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc ccagcaggta
     4561 cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac gccagatgaa
     4621 caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac ggatggaacc
     4681 ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt ccgcttcgcc
     4741 agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc ccatgcgttc
     4801 cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg ccatcggtga
     4861 cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt tatagaaata
     4921 agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg ccagcggctg
     4981 ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc cgcccccgct
     5041 ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt tcatatcgag
     5101 gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt gcgttttatc
     5161 agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa cgccggtaat
     5221 ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg cacagttaac
     5281 gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa cggtggtcac
     5341 cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt gcagcagcac
     5401 gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt acgggaagcc
     5461 tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg tttcttcgcc
     5521 agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc ggaagccaat
     5581 agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt tccattccgg
     5641 attgttttcc tggcccagcg catcaaccag atcagcagcg cgcaacatac gacctgcagc
     5701 gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt agcgacgcac
     5761 atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca ttacgtggcc
     5821 catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca gatcgcacag
     5881 tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt tgtaacgcac
     5941 ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac cccaggcgat
     6001 gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct cgccccaggt
     6061 ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc aagtaccgcc
     6121 aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg ccggaattgg
     6181 cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt tagatgcggc
     6241 gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc cacgcgcctg
     6301 cttaaagctt ttctctttat cggcgtcttc aatgatagaa gcccatgcct caaccggatc
     6361 gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca tcatcgggta
     6421 tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg
     6481 aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct gggtttccca
     6541 ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc agtttacccc
     6601 gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat cctcccagtc
     6661 acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct tctgtttgaa
     6721 gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc ctgtcggccc
     6781 tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga
     6841 attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa gatacccctt
     6901 taggataatt aacagccacc acataacata ctgtaaatta agacgtttta tacctgtagt
     6961 tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc catgggtaac
     7021 attttaatgg ctcttaatga aatccggcga aataaaccat ataacattag tatgttgtaa
     7081 ctaaataacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc aaatatttta
     7141 ctctatataa ttcggtttac aggaaggcgg caagtgaatg aatcccagga gcttactaaa
     7201 gtaagtgact ggggtgaatg aacgcagccg cagcacatgt aacccgaagt atgacgagta
     7261 acggtttatt ttttagaatg ccgaccatat accgcccagg taatcactac gcaggcgata
     7321 tagaaaatca aaaatacctt cattgcgccg actggcgaac ccgttaatgc cagcgagcta
     7381 ccaaacgctt tcgggataaa gaagccacca atcgcgccaa tcgcagagat gaaacccagc
     7441 gccgccgccg tgtcggttgc cgcttcacgc atcgcacgtt cgtcagaacc cccttctgct
     7501 ttcacgcgat ccattgtcag tttacggaag atcactgaaa tcatctggaa agtggaacca
     7561 ctacccagcc cagctgtcag gaacagcgcc aggaagaccg cgaagaacgc catgaagctt
     7621 ccgccctgcc cgtcagtcgg taaggtcagg aacagcaggc cgctgaaaat cgccatcaga
     7681 ataaagttca ccagcgtgac acgagttccg cccagacggt cagataatgc accacctgca
     7741 gaacgcgcca gcgcaccaat aaacggcccg aagaaagcgt attgcagaat ctgaacatcc
     7801 gggaactgcg tttttgacag cattgcaaag cccgcggaga agccgatgaa ggagccgaag
     7861 gttgccagat acagcaggct cataatccac agatgacccc gtttgagtac cggcaactgc
     7921 tccttgatgg aggctttcga ggtagcaaga tcgttcatgc caaaccacgc cgcaatggtg
     7981 aagatggcaa ggaacggcac ccatatccag gacgcattcg ccagatacag ctcagtccca
     8041 tccggctgtt tgacgccctg gctaccaaat actgcgaaaa tcgacagtga taccaccagc
     8101 ggagcaacca actgcatgac gctgacgccc atgtttccca gaccaccatt cagacccagc
     8161 gcgccaccct gcttctgttt cggaaagaag aagctgatgt ttgccatact ggatgcgaag
     8221 ttcgcgccag caaagccgca tagcagagag atgatgatga agacgctata aggcgtggag
     8281 gtatcctgca cggcaaaacc cagccagacg caaggaataa tcagaatacc ggtgctgaac
     8341 gccgtccagc gacgaccacc gaagatagga accataaagg agtatggaac acgtaataac
     8401 gcgccagaaa ccgaaggcag cgcagtcaac ataaatagct gatcggtcgt aaaattaaaa
     8461 ccgactttcg gtaagttcac cgcaacagcg ctgaacaaca tccatacgca aaacgccagc
     8521 agcagacagg gaacggaaat ccacaggttg cggctggcaa tacgttgacc gcgttgttgc
     8581 cagaacgcag gatcttccgg tcgccaatct gtaatgacag ctccagtagc cctttcgggg
     8641 gcggatgagt gactcataga cacctctgat actcgtttcg atgtctgcca ccttagtgtc
     8701 tggagctaaa ggcaatttga tgtaaatcaa acgataagat aactttatct atcattgata
     8761 tttatcatta cccatagtga gtacagtgat ttcataaaaa ttatgagatt tttcacggtg
     8821 ctgtaaaatc cctaccctta ccgatgtaaa gcgactaacc acacggcaaa taaggagtaa
     8881 ctctttccgg gtatgggtat acttcagcca atagccgaga atactgccat tccagaatgt
     8941 atcgtcacat tcattaaggt tattgctcat ttaaagcctg aaggaagagg tttacatgct
     9001 taaacgttgt ctctctccgc tcaccctggt taatcaggtt gcgcttattg tgttgctttc
     9061 tactgctatt ggactggcag ggatggcggt ttctggctgg ctggtgcaag gcgttcaggg
     9121 cagcgcccat gcgatcaaca aagcgggatc gctgcgcatg caaagttacc gtctgttggc
     9181 agcagtgcca ttaagcgaga aagacaagcc cttaattaaa gagatggaac aaacggcatt
     9241 tagcgccgag ttgactcgag cagcagaacg agacggacaa ctggcgcaat tacagggttt
     9301 acaagattac tggcgtaatg aactgatccc tgcactgatg cgtgcacaaa accgcgaaac
     9361 ggtgtcagcg gatgtcagcc agtttgttgc cgggcttgat cagctggtat ctggttttga
     9421 ccgcaccacg gaaatgcgca ttgagacagt ggtactggtc catcgggtaa tggcggtatt
     9481 tatggcactt ttactggtgt tcactattat ctggttgcgg gcgcgactgc tacaaccgtg
     9541 gcggcaactg ctggcaatgg cgagtgccgt cagtcatcgc gattttaccc aacgcgcgaa
     9601 catcagcggg cgcaacgaaa tggcgatgct tggaactgca ttgaacaata tgtctgcaga
     9661 actggccgaa agttatgccg tacttgagca gcgggttcag gagaaaaccg ccgggctgga
     9721 gcataaaaat cagatcctct cttttttatg gcaggctaac cgccgtttgc attcccgcgc
     9781 cccgctgtgt gaacgcctgt cacctgtact caacggctta cagaatttaa ccctgctacg
     9841 tgatatcgaa ttgcgggtgt atgacactga tgatgaagag aatcatcagg agtttacctg
     9901 ccagccagat atgacttgtg atgataaagg ctgccagctc tgcccgcgcg gcgtattacc
     9961 cgttggcgat cgcggcacaa ccctgaagtg gcggctggct gactcacata cgcagtacgg
    10021 tattttgctg gcgaccctac cgcaggggcg tcatcttagc catgatcaac aacaactggt
    10081 ggataccctg gttgaacaac tcaccgccac gctggcgctg gatcggcatc aggaacgtca
    10141 gcaacagttg atcgtgatgg aagagcgtgc caccattgcg cgcgaactgc atgattctat
    10201 tgcccaatct ctctcttgca tgaagatgca ggtgagttgt ttacagatgc agggcgatgc
    10261 gctgccagaa agcagccgcg aactgttaag tcagatccgt aacgaactga atgcatcctg
    10321 ggcgcagttg cgtgaattgc tcaccacatt ccgcttgcag ctcaccgagc ctggattacg
    10381 tccggcgctg gaagcgagtt gcgaagagta cagcgccaaa tttggcttcc cggtgaagct
    10441 ggattatcaa ttgccgcctc gtctggtgcc ttcacatcag gcaatccact tgttgcaaat
    10501 tgcccgtgag gcattaagta acgccctcaa acattcgcaa gcgagtgagg tcgtggtgac
    10561 ggtggcgcaa aacgataatc aggtcaaact gaccgtccag gataacggct gcggcgtgcc
    10621 tgaaaatgcc atccgcagca atcactacgg catgataata atgcgcgacc gtgcgcaaag
    10681 tttacgaggc gattgccgcg tccgccgtcg tgaatcaggt ggcaccgaag tggtggtcac
    10741 ctttattccc gaaaaaactt tcacagacgt ccaaggagat acccatgagt aatcaggaac
    10801 cggctactat cctgctgatt gacgatcacc cgatgctgcg aactggcgta aaacagctta
    10861 tcagtatggc accagatatc accgtggttg gcgaagcgag taatggcgaa cagggtattg
    10921 aactggcgga gtctcttgat cccgatctga tcctgttaga tctcaatatg cccggcatga
    10981 acggtctgga aacgctggat aaactgcgcg aaaagtccct ctcagggcgc attgtggtat
    11041 tcagcgtctc taaccatgaa gaagatgtgg tcaccgcact gaaacgcggc gcggatggct
    11101 atctgttaaa agatatggaa ccggaagatc tgctgaaagc attgcatcag gcagctgctg
    11161 gcgaaatggt attaagcgaa gcattaacgc ctgttctggc cgccagcttg cgcgctaacc
    11221 gtgccactac tgagcgcgat gttaaccagt taaccccacg cgagcgcgat attctcaagc
    11281 tgattgccca gggtttgccg aacaagatga ttgcccgccg cctggatatc accgaaagca
    11341 cagtaaaagt gcacgtcaag cacatgctga agaaaatgaa gctcaagtct cgcgtggaag
    11401 cagcggtatg ggtgcatcag gagcgcattt tctgattacg gttcccagcg cagttcgtcg
    11461 tttgacaatg cgtcgaacgg ttcgacaagc gttagcgtga tctcattgga ggagacacgc
    11521 tgcccctggt tatcttccac caccaccgac aatcgccagt gattgctcgc cccttccccg
    11581 ttctgccagt caggcatgat cagcgtccag ccctccgcgc tgttggcttg tgcgcctggc
    11641 gtcaaactta atatctgcgt atcgccctgc caaatcagtt gccgaatacc gtaacgactg
    11701 cggatttgta atttcagcgg cactgtttcg ccaggtttta gatcccacgg cggtgtcgcc
    11761 agaaacaccg ttaacgtttt tcgctgtcgg tactcaagag tcggtagatt atttcgctgc
    11821 ggattatcat agcgactacc acgtaacgac tgactttcgg caacctcgcc cgcagaaagt
    11881 tgttttttga gcggtacacc aaagcggtaa ttaagattca gcccgaggtt attttgattc
    11941 tcgccacttt caccctgttt atgctgggcc gtcacagtga ctaatggcac aggggtgtaa
    12001 tttaatccca gactcaacgc gacgggattg tgataacccg taccagagtt aaacaaatca
    12061 acacgatcac caaaatactg ttctacgctg acactggtat tgaggtgttg atagaacggc
    12121 atgcgcatcc gggctgtcag gtcgtacccg cgcaccatcc gttgttcctg cgtggctgtc
    12181 tgttcatgcc atgcagcaaa tggctgataa aagtttgccg atagtcgcaa atattcgccc
    12241 cacgcttcgg caccaaagcc cgctcgctga agattttcgt ccagcaagtt gtcataaaaa
    12301 gtgttataac ccaccagcca gttgccgcgc gcccagcgtt gcccaacgcc cacattgctc
    12361 accaacccat catcctgctg agtaagacca agctggctcc aggtgagata acgatcatta
    12421 tcttgtaacg gcacaaacca gcttccacga ctgccggtaa aatgaccttc gttatccact
    12481 ttgacatcaa cactggcatt tccccacggt gatagccagg actctacgtg ctgattaacc
    12541 tgttgactaa gcgcgtcgcg gacttttccc aatgcgaaag cttttgcctg ctcgccagta
    12601 tccagcccgt tatcattcat actggtttcg ccgaaatctt tcacaatttc agcaaagtgt
    12661 ttttccccat catgattttc aggtgccatg cctaaatccg gcagaccatc attgttatta
    12721 tcaaagggat ttgccgcttt ttgctcgaag gtagattgtg cgttagctgt accgcctgcc
    12781 gccagcaaga gtaaataaaa cgaaataatg cgaggaacga aacggctcaa aacgtcggta
    12841 acttccggta aaaaatgaaa aaccatatcc tttaagaata acgattactg gcaacttttg
    12901 tcgtattttc tcgcgctttc aggaaaatcc ttaaaaatgt gaccgattgg tcagacgctg
    12961 cagaagcagt aagatagaag atgagtgtct aattccctga atacaggagt aaataatgca
    13021 aaaaatcgtg atcgttgcca atggcgcacc ttacgggagc gaatccttgt ttaacagctt
    13081 gcggctggcc attgcgttac gagagcagga gagcaatctg gatctgcgtc tgttcctcat
    13141 gtctgatgcg gtcacagccg ggttgcgcgg gcaaaaacca ggggaaggct acaacattca
    13201 gcaaatgctg gagatcctta ccgctcagaa tgtaccggtg aaattgtgca aaacctgtac
    13261 cgacgggcgc gggattagca cacttcctct tattgatggg gtggaaatcg gtactcttgt
    13321 ggaactggcg caatggacgc tgtcagccga taaagtgctc acattttaat gtttgagggg
    13381 gcgtctgacg ccccactatg agggaagttt actcaggcga atcccggccg taacacacca
    13441 tccggataat tctccgccag cagttttttt accgatacca gcaaatcatt cagcccatcg
    13501 tcctgcattc ccagtttgat aagctcctgt tccagtgaaa acaggtattg tgcgttggtt
    13561 cccagcggac cgctcgccgc cgcaatcaac ggcgcgatga cctgagcgcg agtgtcagat
    13621 tcatattctg gatgtcgcgg gtccataata aacacaatgg cgtttactgt gcatccatca
    13681 tcaagatcaa gctgacacca ggttggcaga taacagccgg taatcatctc gcgcttccac
    13741 aacagggtta gttcctgctc cagcgtctct tctggcagtc gataggcgac gcctgtagtg
    13801 cgtccgccct ctttcagtgc aagcatccgt cccggctggt gcgcagtccc gcgcccggcg
    13861 gtcaggcgca ggcaaaacgc gcgatgccat ccaaccagtg taccggtgca cgactcggta
    13921 aactccagtg ccggattcca catcaacgat ccataaccga agatccacac cggtccctca
    13981 tcaggtcgac aagccagcgt cgccgccagc gaagccgccc gttgctctgc tgaccataag
    14041 agtgattctt caatggcacc aaatgccgtt ttacaatcgg cattcatcaa gaaatcacgc
    14101 gttatcacct tacacctccg ccactgcata cttccctgcg acaacttttt ggtgtcttcc
    14161 ggacattttt actgctcatt ttgaaaccac taacgggaca atatgcaatc cacggaccac
    14221 ttgcaaggca acaccgcacg aatagcttta actaagctac cggttttacg attttttatg
    14281 ccatttatca tcatcccctt tggcatattc atgcttcaca gcagcccagg ccactttatg
    14341 cgcggtttct tcgcgactgg cgtcatcacg ccgatcctct ttatctttat attgatccca
    14401 cgcgctgttg aacgcttctt tatagatatc ctgggcatga gacggtagaa cgtgctttac
    14461 gctttccggc agatcgcttt tcgttttata cggcatcgtt gactcctttt acaaagttga
    14521 acattaagtg tggtcaacaa tatctactct tgccaaaaca taagaatttt catgtcagac
    14581 tgttcttaag aaacgctaaa acaaacattg tctaaacgta atacttattt ttatgtccca
    14641 atgagtaaaa aatcgacagt ggggtaaaaa ttcgtaaaat gttaccgata tccagacact
    14701 ttctgttatt tttcctacct attttttaca ttttcgataa attcatctgc aaagggataa
    14761 ccataatgtc aaatgctcaa gaggcggtaa aaacccgcca caaggagact tcgcttattt
    14821 tcccggttct ggcgctggta gtgctgttcc tgtggggaag cagccagaca ctaccagtgg
    14881 tcattgccat caatcttctt gcgcttattg gtattttaag tagcgccttt agtgttgtcc
    14941 gtcatgcgga cgtattagcc catcgcctgg gagaacctta cggttcgctt attcttagcc
    15001 tttcagtggt tattcttgaa gtcagtttga tttcagcttt aatggcaacc ggcgacgccg
    15061 cgccaacgct aatgcgtgat acgctctatt caatcattat gattgttacc ggtgggctgg
    15121 ttggcttttc attattgttg ggcggtcgta agtttgccac ccaatatatg aatctgtttg
    15181 gtatcaagca gtatttaatt gcgctgttcc ccctggcgat aatcgtactg gtatttccaa
    15241 tggctctgcc tgcggcgaat ttttcaaccg gtcaggcgtt actggtagca ttaatttctg
    15301 cggcaatgta tggcgtattt ttgctgatcc agaccaaaac gcatcaaagt ttgtttgtct
    15361 acgagcacga agatgacagt gatgacgacg acccgcatca cggtaaaccg tctgcccata
    15421 gcagcgtgtg gcatgctatc tggttgatta tccatctgat tgccgttatt gcagtcacca
    15481 aaatgaatgc cagcccgctg gagacattgc tcgacagcat gaatgcccct gtcgccttta
    15541 ctggcttcct ggtggctctg ttgattctgt cgccggaagg tttaggtgcg ttaaaagcag
    15601 tgttgaacaa ccaggtccag cgcgcgatga atctgttctt tggttcagtg ttagcaacca
    15661 tttcgcttac cgtacctgtc gtcacgctaa ttgcctttat gacgggtaac gaattacagt
    15721 ttgcacttgg tgcgccagaa atggtggtga tggtggcctc tttagtgctg tgccatatct
    15781 ccttctccac cggacgtact aacgtgctca atggcgcagc gcatctggca ctgtttgccg
    15841 cctatttgat gacgatattt gcctgacagt tatatctcct ccgatgacgg aaatcggggg
    15901 agataagata aattcctgct tattgtattt cttcatacta cctggacagc tcgttaatca
    15961 gaattgcgag cctgtaccac aacctgcacg aaaccactgc aacagctgtt gaaaagccgg
    16021 aatgtactct ttattgcaaa catttcaggg atgaagtgaa gatgaattga gaagtagtat
    16081 ccacaccaag ccggaattat ccggtgaggc gcaatgttgc gggggcttta tccctggtgg
    16141 cattggttgc tggaaagaga aaacccccgc acgttgagag gtaaaacctg acaacaaaac
    16201 gggggctaat cttgaaacca gacacttcaa gaatagccgc aaaatgttgt cattacaaca
    16261 aggcggctat atgacgctcg cgcagtttgc catgactttc tggcacgacc tggcagcacc
    16321 gatcctggcg gggattatta ccgcagcgat tgtcagctgg tggcgtaacc ggaagtaagg
    16381 tgaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc gcaggggtaa
    16441 cagcctgcgg ccatttttgt ctttatctct tccacaagta aatattggca acccgagctg
    16501 gaagcatcat ccagagcact catgaaacga ctgcaacagc tgttgaaaag ccggaatgta
    16561 ctctttattg caaacatttc agggatgaag tgaagatgaa ttgaga
//
LOCUS       DAZFZC010000100        16464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFZC010000100 DAZFZC010000000
VERSION     DAZFZC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.101"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1057
                     /locus_tag="ACHKXP_003928"
     CDS             <1..1057
                     /locus_tag="ACHKXP_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7648845.1"
                     /translation="DILLLTICAVISGAEGWEDIEDFGETHLDFLKQYGDFENGIPVH
                     DTIARVVSCISPAKFHECFINLMRDCHSSDDKDVIAIDGKTLRHSSDKSHRRGAIHVI
                     SAFSTMHSLVIGQIKTDKKSNEITAIPELINMLDIKGKIITTDAMGCQKDIAEKIQKQ
                     GCDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPD
                     ELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSVDLTAEKFATAIRNHW
                     HVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMQKA
                     AMERNYLASVLAGNGLS"
     gene            1328..3565
                     /gene="nrfB"
                     /locus_tag="ACHKXP_003929"
     CDS             1328..3565
                     /gene="nrfB"
                     /locus_tag="ACHKXP_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO7648846.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3552..6524
                     /gene="nfrA"
                     /locus_tag="ACHKXP_003930"
     CDS             3552..6524
                     /gene="nfrA"
                     /locus_tag="ACHKXP_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO7648847.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSDVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6525..7415
                     /gene="ybcH"
                     /locus_tag="ACHKXP_003931"
     CDS             6525..7415
                     /gene="ybcH"
                     /locus_tag="ACHKXP_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO7648848.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNEQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7598..8359
                     /gene="envY"
                     /locus_tag="ACHKXP_003932"
     CDS             7598..8359
                     /gene="envY"
                     /locus_tag="ACHKXP_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO7648849.1"
                     /translation="MQLSSSKPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8872..9825
                     /gene="ompT"
                     /locus_tag="ACHKXP_003933"
     CDS             8872..9825
                     /gene="ompT"
                     /locus_tag="ACHKXP_003933"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO7648850.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPTGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            10012..11496
                     /locus_tag="ACHKXP_003934"
     CDS             10012..11496
                     /locus_tag="ACHKXP_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7648851.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(11680..11880)
                     /locus_tag="ACHKXP_003935"
                     /pseudo
     CDS             complement(11680..11880)
                     /locus_tag="ACHKXP_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            11872..11967
                     /locus_tag="ACHKXP_003936"
                     /pseudo
     CDS             11872..11967
                     /locus_tag="ACHKXP_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12042..12710
                     /locus_tag="ACHKXP_003937"
     CDS             12042..12710
                     /locus_tag="ACHKXP_003937"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7648852.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVIFSA
                     SGKK"
     gene            complement(12655..12792)
                     /locus_tag="ACHKXP_003938"
     CDS             complement(12655..12792)
                     /locus_tag="ACHKXP_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO7648853.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKKLLQYLEPW
                     F"
     gene            complement(12765..13349)
                     /locus_tag="ACHKXP_003939"
     CDS             complement(12765..13349)
                     /locus_tag="ACHKXP_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7648854.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(13349..>16464)
                     /locus_tag="ACHKXP_003940"
     CDS             complement(13349..>16464)
                     /locus_tag="ACHKXP_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7648855.1"
                     /translation="EDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSA
                     REAATHATDAAGSARAASTSAGQAATSAQSASSSAGTASTKATEASKSAAAAESSKSA
                     AATSAGAAKTSETNAAASQKSAATSASTATTKASEAATSARDAAASKEAAKSSETNAS
                     SSASSAASSATAAANSAKAAKTSETNAKASETAAGQSASAAAGSKTAAALSASAASTS
                     AGQASASATAAGKSAESAASSASTATTNAGEAAVQASAAARSASAAKTSETNAKASET
                     SAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTKATEAAGSATAA
                     AQSKSTAESAATRAETAAKRAEDIASVVALEDASTTKKGVVQLSSATNSTSETLAATP
                     KAVKSAYDNAEKRLQKDQNGADIPDKDRFLSNINVYSKGEVDQKKGMRYVVVNAPAGV
                     QEGKYYPLVIKRNDSHRASRVVISTPSRSANHRMNNCEFNGFVCAGGWTDRGSYACGM
                     FWAYSTSERAIHSILMSNKGDTVDSVFYIEGGAFPVEVFLEEGLSVTAPASDYIVAET
                     TYKFGATDPYSESVAVNLILDFKQGNGFYSSYPVLSKSDISGNKIYANDEVIVRSQNA
                     LRMIAGDYGVIWRNDGANTYLLMTDKGDQYGGWNGLRPFAVNNATGEVTINTPLNSPK
                     GIKGNSDTATKLQTAIKISGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNA
                     ESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEV
                     YTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQAFDKSAYPKLAAAYPSGVIPDMRG
                     WTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTH
                     SLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTS
                     TDGNHSHSLSGTAVSAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAF
                     NYIVRLA"
BASE COUNT         4305 a         4081 c         3900 g         4178 t
ORIGIN      
        1 ggatattcta ctgttgacta tttgtgccgt tatttctggt gcagaaggtt gggaagatat
       61 agaggatttt ggggaaacac atctcgattt tttgaagcaa tatggtgatt ttgaaaatgg
      121 tattcctgtt cacgatacca ttgccagagt tgtatcctgt atcagtcctg caaaatttca
      181 cgagtgcttt attaacttga tgcgtgattg tcattcttca gatgataaag acgtcattgc
      241 aattgatgga aaaacgctcc ggcactcttc tgacaagagt caccgcaggg gagcgattca
      301 tgtcattagt gcgttctcaa caatgcacag tctggtcatc ggacagatca agacggataa
      361 gaaatctaat gagattacag ctatccctga acttattaac atgctggata ttaaaggaaa
      421 aatcatcaca accgatgcga tgggttgcca gaaagatatt gcagagaaga tacaaaaaca
      481 gggatgtgat tatttattcg cggtaaaagg aaaccagggg cggctaaata aagcctttga
      541 ggaaaaattt ccgctgaaag aattaaataa tccagagcat gacagttacg caatgagtga
      601 aaagagtcac ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact
      661 tattgatttc acgtttgaat ggaaagggct aaagaaatta tgcgtggcag tctcctttcg
      721 gtccataata gcagaacaaa agaaagagcc agaaatgacg gtcagatatt atatcagttc
      781 tgttgattta accgcagaga agttcgccac agcaatccga aaccactggc acgtggagaa
      841 taagctgcac tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa taagaagagg
      901 aaatgcagca gaattatttt cagggatacg gcacatcgct attaatattt tgactaatga
      961 taaggtattc aaggcagggt taagacgtaa gatgcaaaaa gcagccatgg agagaaacta
     1021 tctggcgtca gtccttgcgg ggaacgggct ttcgtaatct tgccctgcct gctcacctcc
     1081 tccaggcact tccactacat gatgacctgc tcggtgccac cgctcctttt cactatgacg
     1141 ttaacctccc cgtcccagga caccatgctt ttgtctacat tctacgcaaa cggcaacttt
     1201 tataaccagt gtaaaaataa cgtgccgcaa tagtcatacc tattaatggt aaaaagctgt
     1261 cacaattcat aaaaaacctt aatatacgcc accctaaaca taaccagcgt taatgtaagg
     1321 tttttgtgtg gactggcttc ttgatgtttt tgctacctgg ctctacggct taaaagtaat
     1381 cgcgataacg ttagcggtca tcatgttcat cagcgggctg gacgattttt ttattgatgt
     1441 cgtctactgg gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct acccgcgaat
     1501 gagttaccgc gaactgtata aaccagatga aaaaccgtta gcgattatgg ttccggcatg
     1561 gaatgaaacg ggcgtcatcg gcaatatggc cgagctggcg gcgaccacgc tcgactacga
     1621 aaactatcat atctttgttg gcacctaccc caacgacccc gatactcagc gtgatgttga
     1681 cgaagtgtgc gctcgcttcc cgaatgtgca taaggtagtc tgcgcgcgtc ctggccccac
     1741 cagcaaagcc gactgtctga acaacgtgct ggacgccatc acccaatttg agcgtagcgc
     1801 caatttcgct tttgctggtt ttattctgca tgacgccgaa gatgtgattt caccgatgga
     1861 attgcgtctg ttcaactatc tggtcgagcg taaagatctg attcagatcc cggtgtatcc
     1921 gttcgaacgc gaatggacgc acttcaccag catgacttac attgatgagt tttcagagct
     1981 gcatggcaaa gatgttccgg tgcgtgaagc cctcgccgga caagtgccca gcgcaggcgt
     2041 cggcacctgt ttcagccgcc gcgccgtgac cgcactgtta gctgacggtg acggtattgc
     2101 tttcgacgtg cagagtctta ctgaagatta cgacattggc ttccgcctga aagaaaaagg
     2161 tatgacggaa atttttgtcc gttttccggt ggtggacgaa gccaaagaac gcgagcagcg
     2221 taaattttta cagcacgcgc ggacatcaaa catgatctgc gtgcgcgaat atttccccga
     2281 taccttttcg actgcggttc gacaaaaatc ccgctggatc atcggcattg ttttccaagg
     2341 ctttaaaacc cataaatgga cctccagcct gacgctgaac tactttctct ggcgcgaccg
     2401 caaaggggca atcagtaact ttgtcagctt cctcgcgatg ctggtgatga tccagctttt
     2461 gctgttgctg gcgtatgaaa gtttgtggcc cgatgcctgg catttccttt ctattttcag
     2521 cggcagcgca tggttaatga ccctgctgtg gctaaacttt ggtttgatgg ttaaccgcat
     2581 cgtgcagcgg gtgattttcg ttactggcta ctacggcctg acgcaggggc tgctttccgt
     2641 cctgcgtctt ttctggggca acctgattaa cttcatggcc aactggcgcg cgctaaaaca
     2701 ggtacttcaa cacggcgatc cacgtcgcgt ggcgtgggat aaaacaacgc atgacttccc
     2761 cagcgtgact ggcgataccc gctcgttgcg cccgttaggt caaattctgc tggaaaatca
     2821 ggtcatcact gaagaacaac tcgatacagc actgcgtaat cgcgtcgaag gtctacgcct
     2881 gggcggttca atgctgatgc aggggctgat tagcgccgag cagctggcac aggcgctggc
     2941 agagcaaaac ggcgtggcgt gggaatccat cgatgcctgg cagatccctt cctcgctgat
     3001 tgccgaaatg ccggcctccg tggcgctgca ttatgcggta ctgccgctgc gtctggaaaa
     3061 tgacgagtta attgtcggca gtgaagatgg tattgacccg gtttcgctgg cggccctgac
     3121 gcgtaaagtc ggacgcaaag tgcgttacgt cattgttctg cggggacaaa ttgtcacagg
     3181 gttacgtcac tggtatgcac gccgacgcgg tcacgatccg cgggcaatgt tgtacaatgc
     3241 ggttcagcat cagtggctca cggaacagca ggccggtgaa atctggcggc aatatgtgcc
     3301 gcatcagttc ctgttcgccg aaatactgac cacgctcggt catattaatc gttcagcaat
     3361 taacgtgttg ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt ttttggtcac
     3421 cgaaggcgtt atcagccagg aaacgttgga tcgcgtcctg acaattcaac gcgaattaca
     3481 agtttcgatg caatcactat tactcaaagc aggtttaaac acagaacagg ttgcgcaact
     3541 ggagtccgaa aatgaaggag aataacctta atcgcgtcat cggatggtct ggtttactgc
     3601 tgacgtcttt attgagtacc agcgcactcg cagacaatat cggcaccagc gcagaagagc
     3661 tgggtctgag cgattatcgc cattttgtta tttatccccg tctcgataag gcgctgaagg
     3721 cacagaaaaa taacgacgaa gcaaccgcca tccgcgaatt tgaatatata caccagcagg
     3781 tgccggataa tattccgctg actttatacc ttgcggaagc ctatcgccat tttggtcatg
     3841 atgaccgggc gcggctgttg cttgaggatc aactgaaacg tcacccagga gatgcccgac
     3901 ttgagcgcag tctggcggct attccagttg aagtgaaaag cgttacgact gttgaagaac
     3961 tgcttgccca gcaaaaagcg tgcgatgctg caccgaccct gcgttgtcgc agtgatgtcg
     4021 ggcagaatgc cctgcggctg gcacagttac ctgtcgccag agcgcaactg aacgatgcga
     4081 cgtttgctgc atcgccggaa ggaaaaacgc tgcgaaccga tctgctgcaa cgggcaatct
     4141 acctgaaaca atggtcccag gcagatacgc tatacaatga agcacgccag cagaacacat
     4201 taagcgcggc agaacgccgt cagtggtttg acgtgcttct tgccgggcag ctagacgatc
     4261 ggatcctggc actgcaatca caggggatct tcaccgatcc tcagtcatat attacttacg
     4321 cgaccgcgct ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc aatgaaaata
     4381 agccgctgtt taccacggac gcacaagaga aaagttggct ctatctgtta tctaaataca
     4441 gcgccaaccc cgttcaggca ttggcgaatt atacggtaca gtttgccgac aaccgccagt
     4501 atgttgttgg cgcgacgcta ccggtgctgt taaaagaagg tcagtacgac gcagcgcaaa
     4561 aactgctcgc caccctcccc gccaatgaaa tgcttgagga gcgttatgct gtcagcgtgg
     4621 cgacccgtaa caaggctgaa gctctgcgtc tggcacgctt gctgtatcag caagaaccgg
     4681 caaatcttac ccgcctggat caactaacct ggcaactgat gcagaacgag cagtcacgcg
     4741 aagctgccga tttattgctg caacgctatc ctttccaggg cgatgcgcgt gtcagccaga
     4801 ctttaatggc gcgactggcg tctctgctgg aaagtcatcc ttacctggca acgccggcga
     4861 aggtggcgat tttatcgaaa cccttaccgc tggcggagca acgtcagtgg caaagtcagt
     4921 tgccgggtat tgcagataat tgcccggcaa tagttcgctt gctgggcgat atgtcgcctt
     4981 cctacgatgc cgccgcctgg aaccgtctgg caaagtgtta tcgggacacg ctacccggtg
     5041 tggcgttgta tgcatggctt caggccgaac aacgacaacc gaacgcctgg caacatcgtg
     5101 cggtagccta tcaggcgtat caggttgagg actacgccac cgcactggcg gcctggcaga
     5161 aaatcagtct tcacgacatg agcaatgagg atctgcttgc tgctgccaat accgcccagg
     5221 cggcaggaaa tggtgcggct cgcgatcgct ggctacaaca ggcagaaaaa cgtggactgg
     5281 gaagcaatgc gctctactgg tggctgcatg cgcaacgtta cattcctggt cagccggaac
     5341 tcgcactgaa cgatctcacg cgctcaatca atattgcgcc ttctgccaac gcttacgttg
     5401 cgcgggcgac aatttatcgc caacgtcata atgtcccggc cgcggtgagt gatttgcgcg
     5461 ccgcgctgga actggaaccg aataatagca acacccaggc agcgcttggt tacgccttgt
     5521 gggatagcgg tgatatcgca cagtcgcggg aaatgctcga acaggcgcat aaagggctac
     5581 cggacgatcc ggcactgatc cgacaactgg cctatgtgaa ccagcgtctg gatgacatgc
     5641 ctgcgacgca gcactacgcc cggctggtga ttgatgacat tgataatcag gcgctgataa
     5701 ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtttg catgaggagg
     5761 tcggtcgccg ctggacgttc agtttcgatt cttctatcgg cttgcgttcc ggggcaatga
     5821 gcaccgctaa caataatgtc ggcggcgcag cgccagggaa aagctatcgt agctacgggc
     5881 aactggaagc cgagtaccgc atcggacgca atatgctgct ggaaggcgac ctgctctcag
     5941 tttatagccg cgtctttgcc gataccggag aaaacggggt gatgatgccg gtgaaaaatc
     6001 cgatgtccgg caccggtcta cgctggaagc cgctgcgcga tcagatcttt ttcctcgccg
     6061 tcgaacagca gttgccgctg aacgggcaaa atggcgcatc cgataccatg ctgcgcgcca
     6121 gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac ggttcaggct
     6181 ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat attcaggcgt
     6241 ggacggcaga ttatcgcgtc agctggcatc agaaggtagc taacggacag actattgagc
     6301 cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc gcgcagcttg
     6361 gcggtgtcgg ggtccgctgg aatatctgga ccggcgagac gcactacgac gcctggccgc
     6421 acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat caacgtaacg
     6481 gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt aagttcattt
     6541 tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa ggtattatct
     6601 ggcaaccaca aaaccgtgat agtcaggtta ccgataccca gtggcagggg ctgatgagtc
     6661 agttacgttt gcaaggcttc gataccctgg ttttgcaatg gacccgttac ggcgatgcat
     6721 tcacccagcc agaacagcgc gcgttattgt ttaagcgggc cgcagctgcg caacaggctg
     6781 gcctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac cagaaacagt
     6841 cgtccgcagc gctggaaagc tatcttaatc gcctgctggc tgccgatctc cagcaagcca
     6901 gattatggag cgccgcgcct ggcgtaacgc cggatggctg gtacatcagc gcggaaattg
     6961 acgacctgaa ctggcgcagc gaagccgccc gtcagccttt gctaacatgg ttaaacaacg
     7021 agcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt tttttcgccg
     7081 gaaacatgtc gcccgatggc tatcgccaac tgctggaaca cgttaaagca accggcgtta
     7141 atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag cgtgaacgtt
     7201 atctacaggc cagcgccgat tgccaaagct ccgccccagc aagcggcatt gtttatgaac
     7261 tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggac gcagaaattg
     7321 cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacac tctctatttc tctctgcgct
     7381 atttgcccgt cgcgcacggc attctcgagt attaaatctc ctccaggtaa gtcgggtacg
     7441 acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc taaaaagcgt
     7501 gctaaagaaa gaagatcgtt atcggcgaac gggccgatca ctcatgcagg aagcatagat
     7561 cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca gcagtaaacc
     7621 ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc atgctacgtt
     7681 tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca ttgaactctc
     7741 aacgttaaat cacgtattaa tcacccacat caaccgtaac atcatcaacg attatctgtt
     7801 gtttttaaat aagaacttaa cctgtgtaaa gccctggtcg cggctggcta ccccggttat
     7861 cgcttgtcat agccgtacac cggaagtgtt ccggctagcc gccaaccaca gcaagcagca
     7921 acccagcaaa ccctgcgaag cggagttgac gcgcgcattg ctttttaccg tattgtctaa
     7981 ctttcttgag caatcgcggt ttattgccct actgatgtat atcttacgca gcagcgtccg
     8041 cgacagcgtc tgccgcatta ttcaaagcga tattcagcac tactggaatc tgcgaattgt
     8101 cgccagttcg ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa acgaaaatac
     8161 cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga tgttattgat
     8221 ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca cgtcgtactt
     8281 tatctctgtt tttaaggcgt tttacggcct gacaccgttg aattatctcg ccaaacagcg
     8341 acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat aaatgaagaa
     8401 gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagt tcctagaacg
     8461 acattttaag tcaacaaatt accgcgccat ctctgcgctc acacgtccca ctacctcaaa
     8521 acatgtaaag ccttgcaagc cattgtgagg ccttatgtgt ctcagttttg tccctctttt
     8581 ttgtactaaa aaacatagca attgaggata aacctcatgc tattttcgct tatatgcctt
     8641 taaaggcatg gcgcttaaat agataaaaac accacaaaag cataaaaaac cacacagtaa
     8701 aaccgcaaca taaaacaata acagataatt aaaccaaaaa cagatagcgc attgtgataa
     8761 tcattcaata ctaaacaaaa tataaacagt ggagcaatat gtgattgact cattaagtta
     8821 gatataaaaa atacatattc aatcattaaa acgattgaat ggagaacttt tatgcgggcg
     8881 aaacttctgg gaatagttct gacaacccct attgcgatca gctcttttgc ttctaccgag
     8941 actatatcgt ttactcctga caacataaat gcggacatta gtcttggaac tctgagcgga
     9001 aaaacaaaag agcgcgttta tctagctgaa gaaggaggcc gaaaagtcag tcaactcgac
     9061 tggaaattca ataacgctgc aattattaaa ggtgcaatta attgggattt gatgccccag
     9121 atatctattg gggctgcggg ctggactact ctcggcagcc gaggtggcaa tatggtcgat
     9181 caggactgga tggattccag taaccccgga acctggacag atgaaagtag gcaccctgat
     9241 acacaactca attatgccaa cgaatttgat ctgaatatca aaggctggct cctcaacgaa
     9301 cccaattacc gcctgggact catggccgga tatcaggaaa gccgttatag ctttacagcc
     9361 agaggtggtt cctatatcta cagttctgag gagggattca gagatgatat cggctccttc
     9421 ccgactggag aaagagcaat cggctacaaa caacgtttta aaatgcccta cattggcttg
     9481 actggaagtt atcgttatga agattttgag ctaggtggca catttaaata cagcggctgg
     9541 gtggaagcat ctgataacga tgagcactat gacccaggaa aaagaatcac ttatcgcagt
     9601 aaagtcaaag accaaaatta ctattctgtt gcagtcaatg caggttatta cgtaacacct
     9661 aacgcaaaag tttatgttga aggcgcatgg aatcgggtta cgaataaaaa aggtaatact
     9721 tcactttatg atcacaatga taacacttca gactacagca aaaatggtgc aggcatagaa
     9781 aactataact tcatcactac tgctggtctt aagtacacct tttaacgaag ttaaccagat
     9841 tttctccccg gtggtattgt ataaccccgg ggaatttgtt tacagttaca agtaggcaat
     9901 atccactaat tttgtaagca tttaacctat gtcataacgt gtaatctctt accgcaacac
     9961 aagacctcaa tatattcaaa tcttatcttt attccgatat tcagagacac aatgcgaaaa
    10021 ataattactc atttcaaagt tgttttaacg ttacttctac cagtaaccgt atctgcccag
    10081 cagatacagt ggcaatcctg tatggccagt caattcaacc actggtttgg tgaggaaaaa
    10141 ccgtctcctg acttactatg tggttatttg tctgttccat taaaatatac agacacaggc
    10201 ggagatgctt cttatgaaaa aaaatcacaa gtcaaactag cgttgacaaa attgccggca
    10261 aaaagcaagc ataaaggaag tatcctgata ataagtggtg gtcccgggtt accaggcata
    10321 aatccttata ttaactttga ctggccagtc acaaatcttc gtgagtcatg ggatattatt
    10381 ggatttgatc ctcgaggcgt cggacagtcc actccgacaa taaactgccg gcaatcagat
    10441 acagagactc aggaaaacat aaccgaaaag caacaagtat taaataaaat taatgcctgt
    10501 atccataata ccggagccga agtcattcgc catatcggct ctaacgaggc tgtatacgat
    10561 attgatcgta ttaggcaagc cttgggggat aaacaactga cagccgtggc gtattcgtat
    10621 ggaactcaaa ttgcagcctt atatgcagaa cgttttccct acaacgtaag atctatcgtt
    10681 cttgatggag tcgtcgatat cgatgacctg gaggacaact tcacatggca actcaaacag
    10741 gcacagagtt atcaggaaac gtttgatcgc tttgcatcct ggtgtgcgcg tacaaaaagt
    10801 tgcccgcttt cttcagacag agataaggca ataactcagt tccatgagct attatcaaaa
    10861 ttacatcaca aacctttatt agacagtaag agagaaaata tatcttcaga tgaactcata
    10921 tcattaacaa cagaccttct gctatggcgt tcatcatggc caacccttgc aactgccata
    10981 cgccagttct ctcaggggat tgtcagtaat gaaattgaaa ctgcgctcag tgctccgata
    11041 gcctcagaag agtcaagcga tgcttcgggg gtaatcctct gtgtagatca gggggatgag
    11101 caattaacac cagaagagcg aaaatcccga aaagacgctc ttgcgaatgc cttcccggct
    11161 attaactttg acaatggacg ttccgattca cctgattttt gtgaattatg gccaatacat
    11221 agcgacctga acaaaactcg cctgaaaaat actgttctgc cctctggttt actgtttgta
    11281 gcacacaaat acgacccaac aacgccctgg attaatgccc gtaagatggc agagaaattt
    11341 tccagcccgt tactaacaat aaatggtgat gggcatacat tagctctcac cggagttaat
    11401 ttatgtgtag ataaagcagt tgtacatcac ctgatcactc cacaaaaaat agaaaatata
    11461 tactgcccag gaaattctga agcagaaata caataatttc aaaatattcc gctatttgct
    11521 cccaatgcaa aacatatatt gcattgggag catattcata tttttgttat tattgtatga
    11581 aatcgtctca tgtacacggt ccttttttcg ccgaaaactg atattacttt aatcaaaagt
    11641 tgacgtgcca gattcgaagt aaatttataa aatatcaaat caatatacat tttgcacctt
    11701 cgagatagta atgactgtta ctgtaacatg agttacaaac agcttcatac ccagagctac
    11761 tgatgtatca actcgattca acaatacccg atatttattc catgcctcca gcaacgatct
    11821 ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact
    11881 gaattcctag atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgttgtat
    11941 cgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
    12001 tcatcgccat aattgattta attcacaaat aaaactataa catggtgaaa ttaatgaaaa
    12061 aaaacacaga tgatggggct aaaatttaca caccacttac cctaaagctt tatgactggt
    12121 gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa caccttcttc
    12181 cacactttct ggaacattta ggtaacaacc atctggatat tggtgttgga actgggtttt
    12241 accttactca cgtacctgag agtagtctga tatctttaat ggatttgaac gaagctagcc
    12301 tgaacgcggc atctacaagg gctggggaat caaaaattaa acataaaatt agccatgatg
    12361 tttttgaacc ttatcccgcg gcgttacatg gtcaatttga ttccatttcc atgttttacc
    12421 ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc aatgcggcgc
    12481 aggccttaac tgacgatgga actctatacg gagccacaat tcttggcgat ggagttgtgc
    12541 acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca
    12601 caaaagattc cgaagaaggc ttaacacata tactctcaga gcatttcgag aatgttaaaa
    12661 ccaaggttca aggtactgta gtaatttttt ccgcttcagg gaaaaaatag catccaaccg
    12721 cagcacgttc ttgcttaaga cgtgctgcgg cataatccca atgattactc cctgacaggg
    12781 ttcgtaggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga
    12841 tactttttcc aggcttccag caatgaggtt tcttcctccg ttgcgatttc cagatctaca
    12901 gcatcctgaa gtggcgcaat atgctcactg gctacctgca tcaggctgtt ttttatttct
    12961 tccgcctctc ggatccggaa cagtttttct gcttctgcat ctttcaccca ggctgtgccg
    13021 ttccacttct gaaactcccc ttccggcgat aaccaggtaa cattttccgg taacggaccg
    13081 agttcagaaa taaataactc gtcccctgac gctacgtcat aaaccgtttt accccgatgg
    13141 tcttcaacga gatgccacga tgcctcatca ctgttgaaaa cagccacaaa gccagcagga
    13201 atatctggtg gtgcaatatc ggtactgttt gctggcagac ctgtatgagg cggaatatat
    13261 gcatcacctt caccaataaa ttcattagtt ccggccagca gattataaat ttttatggtc
    13321 cgtgcttgtt cactcattct gaatgccatt atgcaagcct cacaatatag ttaaatgcga
    13381 tgtttttgac ggtgttttcc gcgttaccag cagcgttaac ggtgatggtg tgtccatgtg
    13441 accctatcgc aacggagtgc gtatgagcac caataccgac agtatgcgcg tgtgcacctg
    13501 cagatactgc tgtaccggac agtgagtggc tatgattacc atctgtactg gtattagcta
    13561 accaccccgt agacatacct actgagcctt gtacccccca ggtattttga cctgagcttg
    13621 tataaccata ttgataagta tctttaaaaa cactggggtt aaatcgacgg ccatctctat
    13681 ggctgtgatt accagccgca ttcgtgctgc cacttaaact atgggtatgc gcaccggtgt
    13741 tattcgtgga tttagtgccg taatcaaacg acgatgtggt tttcgtcccc aaatccgtac
    13801 tggatgcgct ggcgctgtgg gtatgcgatt taatgccgtc ctgttcctga gacaatacgg
    13861 cccgaccact ggcaggtttg cccttaatcg tccagccacg catatcaggg atcacgcctg
    13921 acggataagc cgctgcaagt ttcgggtatg cagatttgtc aaaagcctgc ccctgcatca
    13981 gggcataacc agacggaacg gtatctgatg gccacggaat tggtgcgccg actgggtagc
    14041 tttctggtgg aagatttttc gaggtataaa cttctgccca gtcttcctca aaaccatagc
    14101 catctcttga ggaacggtag aacagacctc catttctgta atgtgccttc atctgcaagg
    14161 tccggcaact tccgactccg gtatagaagt taaccagaat atagctgtcg ccagagcggg
    14221 tgacattgta agcgcctgat tcggcattcc atggaacgcc cccatccgca tcggcatacg
    14281 tatccgttgc tcttctggca aaagcagcaa catgcgcagc ggttaaagtg atatcagtag
    14341 aaccatcaaa cgaaacacca gatattttta ttgctgtctg aagtttcgta gctgtatcag
    14401 aatttccctt aatcccctta ggagaattaa gtggtgtatt gatcgtcact tcacctgttg
    14461 cgttatttac tgcaaatggt cgaagaccgt tccagccacc gtactggtcg cctttatcag
    14521 tcatgagcaa gtaagtattt gctccatcat ttcgccatat gacaccgtaa tcacctgcaa
    14581 tcatccttaa cgcgttctga ctacggacaa taacttcatc attagcgtaa atcttattcc
    14641 cgctaatgtc tgacttactc aaaacaggat atgaactata aaatccatta ccttgtttaa
    14701 aatctaaaat taaatttacc gcaactgatt cagaataagg atcagttgcg ccaaatttat
    14761 aagtcgtttc agcaacaata taatcagaag caggtgcagt aacggagaga ccttcttcta
    14821 aaaagacctc tacgggaaat gccccacctt ctatatagaa tacgctgtct acggtatcgc
    14881 ctttattact catcagaatg gaatgaatag cgcgttccga tgttgaataa gcccagaaca
    14941 taccacaggc atagctaccc cggtccgtcc agccgcctgc gcaaacaaaa ccattaaact
    15001 cacaattatt cattctgtga ttagctgagc gacttggcgt tgaaatgaca acgcgggatg
    15061 cccgatggct gtcattcctt tttatcacta acggataata tttaccttcc tggacacccg
    15121 ccggggcatt taccacaacg tatcgcatcc cctttttctg atccacttca cctttgctgt
    15181 aaacattaat gttactcagg aagcggtcct tgtcgggtat atcagcgccg ttctggtctt
    15241 tctgcagacg tttctctgca ttgtcatagg ctgattttac tgcctttggc gttgccgcca
    15301 gcgtttcaga cgtactgttg gtcgcactgc tgagctgtac tacccccttt ttcgtcgtgc
    15361 tcgcatcctc aagcgccacg acggatgcaa tatcctctgc ccgttttgcc gctgtctcgg
    15421 cgcgcgttgc cgcggattcc gccgtacttt tgctctgtgc tgccgccgtc gcactgcctg
    15481 ccgcctctgt tgctttcgtg gatgccgtcg tggcgctgct cttcgctgct gacgcctgtc
    15541 tggtcgcctc atcttttgaa gcagacgccg atgatgccga tgacgccgcc gaactggcgg
    15601 acgatgccgc tgccgttttt gaggattctg cgctggtttc cgacgctttc gcgttcgtct
    15661 cggatgtctt cgctgcggaa gctgacctcg ctgctgcgct ggcctgtaca gcggcttcgc
    15721 cagcattcgt tgtggctgtt gaagcagacg atgcagcact ttctgccgat tttccggcgg
    15781 cggtggcact ggctgaggcc tgcccggcac ttgttgacgc ggcactggca gataatgcag
    15841 ccgctgtttt tgagcctgcc gcagctgagg cgctctgtcc cgctgccgtt tcagaagcct
    15901 tggcgttcgt ctcggacgtt tttgccgcct tcgcagaatt tgctgccgcc gttgccgagg
    15961 aagctgcact actggcactt gatgatgcgt tcgtttctga tgatttcgct gcctcttttg
    16021 aggccgccgc atcccgggct gaggtggcag cttctgacgc tttcgtggtc gcggtggatg
    16081 cagaagtggc tgcagatttt tgtgacgctg cagcattcgt ttctgacgtt ttcgccgcac
    16141 cggcactggt ggccgccgcg ctttttgagg actctgcagc ggcagcactt tttgatgctt
    16201 cagtagcctt tgttgatgcc gttcctgcgc tggaagacgc tgactgagcc gacgtcgcgg
    16261 cctgcccggc tgatgtgctg gctgcacgtg ctgagcctgc agcatcagtc gcatgggttg
    16321 ccgcctcacg ggctgatgtg ccggcatcgc tggctgactt cttcgcggct gccgtgttct
    16381 gtgccaccgc ggacgcgtta cgcgccacct cttccaccat cagttcaaaa cggcgcagtg
    16441 cctccggacg ggcatcatcc tccg
//
LOCUS       DAZFZC010000101        16196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFZC010000101 DAZFZC010000000
VERSION     DAZFZC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.102"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            66..335
                     /locus_tag="ACHKXP_003941"
     CDS             66..335
                     /locus_tag="ACHKXP_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7648856.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            304..1422
                     /locus_tag="ACHKXP_003942"
     CDS             304..1422
                     /locus_tag="ACHKXP_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO7648857.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1589..2383
                     /gene="potC"
                     /locus_tag="ACHKXP_003943"
     CDS             1589..2383
                     /gene="potC"
                     /locus_tag="ACHKXP_003943"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO7648858.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2380..3426
                     /gene="potD"
                     /locus_tag="ACHKXP_003944"
     CDS             2380..3426
                     /gene="potD"
                     /locus_tag="ACHKXP_003944"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO7648859.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3484..3945
                     /gene="ymfA"
                     /locus_tag="ACHKXP_003945"
     CDS             3484..3945
                     /gene="ymfA"
                     /locus_tag="ACHKXP_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO7648860.1"
                     /translation="MSQDSKVFFRIFLGIGLVLILISVVVFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKDGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKSRRKRKSRP"
     gene            3942..4730
                     /gene="ycfZ"
                     /locus_tag="ACHKXP_003946"
     CDS             3942..4730
                     /gene="ycfZ"
                     /locus_tag="ACHKXP_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO7648861.1"
                     /translation="MKKFIILLSLLILLPLTAASKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGIAPFIVVLPDINNEASLRQNGKAMLAHASSSLSDVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLHAGLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGKQHENDPPGSTNTLGFIAWAATFVLFSSVFYYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAVLLLTSKR"
     gene            complement(4849..5688)
                     /gene="cobB"
                     /locus_tag="ACHKXP_003947"
     CDS             complement(4849..5688)
                     /gene="cobB"
                     /locus_tag="ACHKXP_003947"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415638.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO7648862.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDITPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGLKAG
                     SIA"
     gene            complement(5704..6615)
                     /gene="nagK"
                     /locus_tag="ACHKXP_003948"
     CDS             complement(5704..6615)
                     /gene="nagK"
                     /locus_tag="ACHKXP_003948"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO7648863.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALIMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6644..7888)
                     /gene="lolE"
                     /locus_tag="ACHKXP_003949"
     CDS             complement(6644..7888)
                     /gene="lolE"
                     /locus_tag="ACHKXP_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO7648864.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQHLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(7888..8589)
                     /gene="lolD"
                     /locus_tag="ACHKXP_003950"
     CDS             complement(7888..8589)
                     /gene="lolD"
                     /locus_tag="ACHKXP_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO7648865.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8582..9781)
                     /gene="lolC"
                     /locus_tag="ACHKXP_003951"
     CDS             complement(8582..9781)
                     /gene="lolC"
                     /locus_tag="ACHKXP_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO7648866.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10043..11116
                     /gene="ycfT"
                     /locus_tag="ACHKXP_003952"
     CDS             10043..11116
                     /gene="ycfT"
                     /locus_tag="ACHKXP_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO7648867.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASSAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLAVLAVAAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SRRAFSDLLFSPPSLPAAVSYSR"
     gene            11244..14690
                     /gene="mfd"
                     /locus_tag="ACHKXP_003953"
     CDS             11244..14690
                     /gene="mfd"
                     /locus_tag="ACHKXP_003953"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO7648868.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFEKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            14834..15796
                     /gene="ldtC"
                     /locus_tag="ACHKXP_003954"
     CDS             14834..15796
                     /gene="ldtC"
                     /locus_tag="ACHKXP_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO7648869.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANLGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(15879..16136)
                     /gene="bhsA"
                     /locus_tag="ACHKXP_003955"
                     /gene_synonym="ycfR"
     CDS             complement(15879..16136)
                     /gene="bhsA"
                     /locus_tag="ACHKXP_003955"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO7648870.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4090 a         4186 c         4056 g         3864 t
ORIGIN      
        1 ttaatgttca accgccgcca gttgtcgttg atattctgca actggcgcgt tccggagtga
       61 tagccatgag cgaacagtac ctgataacgc tcgacgagtg gaaaccaaaa cggttcagtc
      121 tcccaataac aaacactacc ctggtgaaat acggaaaact aggatacatc gttccaagac
      181 cacaaaaaat tcgtgggcgt tggctgatag atcgccgagc agtatttgtt gggcctggtg
      241 aaacgggaat tgcgccggaa attcatactg gcgatgatga tgcactgaag gagattttaa
      301 ctcatgtcac cgaggccacg aaaaaacagc actgacgtag ccggtcttta cgaaaagttt
      361 gatcgcagaa ctggcagagt ttactaccag tataaaaatc ctgtgactgg aaaatttcac
      421 ggactcggaa cagacaaagg taaggcagaa aaaatcgctt ccacagccaa tcagcgaata
      481 gctgcagcag aagctgaata tttcatgcgc aaaattgatg aaagtccgtc agcaacaaaa
      541 cgtcggggta tcagattaaa ggcatgggtt gatcgatatc tgaaaataca ggacacgcga
      601 ctgaaaaatg gagatattgc agctacaact cacaaagaaa aaactcgaat ggctgcatac
      661 ctggtttccc gtctgggaaa ccacccattg aaagaactgg aagtaagaga ctttgcatta
      721 atactggatg agtggctgga taaagacatg gtcagcacag cgagagtaaa tcgtggatta
      781 tgggttgata tttataaaga agcacagcat gcaggggaag ttcctcctgg atggaatcct
      841 ccggaggcta cccgtaaacc gatccctaaa gtaaccagag ccaggctcac catggaagac
      901 tggcaaaaaa tttacaatgc aacgcctgaa aaacacttta tccgtaacgc aatgcttctt
      961 gcgattgtta ctggtcagcg ccgtgatgac atttgccaca tgcgtttttc agatgtgtgg
     1021 aacgaacact tgcatatcac ccagggaaaa accggaatgc gtctggcgtt accgcttaca
     1081 ctacgctgtg atgccattgg gataacgtta aaagaagtta ttgatgggtg ccgagacaga
     1141 atattaagtc catatctaat ccatagtcgg caccagaaac aaccgaagcc gatgagtaaa
     1201 gacaacctga gcgactactt tgccaaagca cgggatctgg ctgggataat tccaccagca
     1261 ggaaaaactc cgccaacatt tcatgaacaa cgctctctat cagaacggct gtaccgtgca
     1321 cagggtatcg atacaaaaac attactagga cataaagtcc aggcaaccac cgatcgctat
     1381 aacgatactc gaggtcagga atgggttaag ttggttattt aacaaacacc atatggaata
     1441 tccgtcacca tctcaggaaa aaagtgttgt aaaatgcgtg gcctagtttt ggggaaacgt
     1501 tttggagaga ttttggagaa aggaaaaaat tgaataaatt caaaatcctg aaagtgtccc
     1561 gccttatgaa taaaaaggtg gaactcgaat gatcggtcga ctgcttcgcg gcggttttat
     1621 gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga actcctttaa
     1681 cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata gcctgctgat
     1741 gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg tgttttcggc
     1801 gacgtttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg
     1861 cggtaagccg ttcgttagcg gaatgctgtt tgtggtaatg atgtcaccag atatcgtgat
     1921 ggcaatttcg ctgctggtgc tgtttatgct gctgggtatt cagcttggct tctggtcgct
     1981 gctgttctcg catatcacct tctgcctgcc atttgtggtg gtgacggtgt attcgcgcct
     2041 gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca gcgaatttac
     2101 cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg gctgggtgtt
     2161 aagctttacc ctgtcgatgg acgatgtggt agtttcttcg ttcgtcaccg gaccaagtta
     2221 tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa
     2281 cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca gccagcttat
     2341 tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac
     2401 ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga tgacaacaac
     2461 acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga acagttcacc
     2521 aaagaaaccg gtattaaggt tatctattcg acttacgagt cgaacgaaac catgtatgcc
     2581 aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac ctattacgtc
     2641 gataaaatgc gtaaagaagg gatgatccag aagatcgaca agtcgaagtt aacgaacttc
     2701 agcaatctcg atccagacat gctcaataag ccgtttgacc cgaataacga ttactccatt
     2761 ccgtatatct ggggtgcgac ggcgattggt gttaacggtg atgcggtgga tccgaaatct
     2821 gtcaccagct gggccgatct gtggaagcct gagtacaaag gcagcctgct gttgaccgac
     2881 gatgcccgtg aagtgttcca gatggcgctg cgtaagctgg gctactccgg taacaccacc
     2941 gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc aaacgtggca
     3001 gcctttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa cctcggcatg
     3061 atctggaacg gttctgcttt cgttgcacgc caggcgggta cgccaattga cgtggtatgg
     3121 ccgaaagaag gcggcatttt ctggatggac agcctggcga tcccggcaaa tgccaaaaac
     3181 aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt
     3241 gccgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt aagcccggaa
     3301 gtggcgaacg ataaaacgct ctacccggat gctgaaacca ttaaaaatgg cgaatggcag
     3361 aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct gaaagcagga
     3421 cgttaattca gcaagcgggc ggtggtatta ccgcccgttt tttagttagc aaggatgtgg
     3481 ctaatgagtc aggacagtaa agttttcttt cgtatattcc tcggtatcgg tctggtactg
     3541 atcctcattt ctgttgttgt cttttataac cagttcacct atagcaaaga cgcgattcat
     3601 accgaaggcg tcattgttga taccgtctgg cactccagtc attctcatcg cacgggtaaa
     3661 gatggttcgt ggtatcccgt cgttgccttt cgaccgacgc cagattacac gctaattttc
     3721 aattcgagta tcggcagcga tttttatgaa gacagcgaag gcgataaagt taatgtttat
     3781 tattctcccg ggcatcctga aaaggctgag ataaataacc cgtgggttaa tttttttaaa
     3841 tgggggttta tcggcattat gggcgttatt tttatcgccg tcggtttatt aataagtatg
     3901 ccctcctcta aaaaatcacg caggaaacgg aagtcccgcc catgaaaaaa ttcattatac
     3961 tgctcagttt actgatactc ctgcctctga cagcagccag caagccatta attccaataa
     4021 tgaagacact ttttacggac gttaccggta ctgtaccaga tgctgaagag atcgcgcata
     4081 aagccgagct ttttcgccaa caaacgggaa ttgcaccgtt tattgtggtt ttacccgaca
     4141 ttaataatga agccagtctc agacaaaatg gtaaagcgat gctggcgcat gcgtcatctt
     4201 cattgagtga tgtaaaagga agtgttctgt tactatttac tacccgcgaa ccacggttaa
     4261 ttatgatcac caacggccag gttgaaagcg gtctggacga taaacatctc ggccttctga
     4321 tagaaaatca cacactggct tatttacatg ctggcctctg gtatcaggga atcaataatg
     4381 cattggctgt tctacaagca cagatattaa aacaaccgac gcctccacta acatattatc
     4441 cgcatccagg aaaacagcat gaaaatgatc cccccggcag caccaacaca ttaggtttta
     4501 tcgcctgggc tgcaacgttt gtcttatttt cctcggtctt ttactacacg actcgtttta
     4561 tttatgcgtt aaaatttgca gtagcgatga ctattgccaa tatgggctat caggctttat
     4621 gcctgtatat cgataatagc tttgcgatta ccagaatatc gccactctgg gcagggctga
     4681 ttggtgtctg tacgtttatt gccgtgttgt tattgacgag taaacggtaa aagagacagt
     4741 aaaatgcgtg tgtattatgc cggttgcggc gcgaacgcct tatccggccc acaaaacccg
     4801 caaattcaat taattgcgtc cccttgcagg cctgataagc gtagtgcatc aggcaatgct
     4861 tcccgctttt aatcccttca gcaacttttc aacaaactca ggcaccacct ggcttgccgg
     4921 gccgtaatat ttctcggcaa attcattacc aacctgactc ggttcaagat tcagttccac
     4981 ggtgtgcgcg ccatgcagtt tcgcttcgtg aacaaaccca gccgccggat aaacatgccc
     5041 ggatgtgcca atggcaatga aaatatcggc catcgacaac gccatataaa tttcatccat
     5101 gccgagtggc atttcgccaa accacactac atgcgggcgc aagggtgccg gaaactggca
     5161 acaatggcat ttatcttctg gggtaatgtc tcctgtccag tcgagaacct gaccactttg
     5221 tgaacaacgc actttcagca gttccccatg catatgaatc acattggtat tacctgcgcg
     5281 ttcatgcagg ttgtcgatat tctgcgtcac cagcaaaaag cgatcgccga gggcgtcttg
     5341 cagtttagcc agcgcaagat gcgcggcgtt aggctgaatt tctggctgct gcagctgtcg
     5401 acgacgggcg ttataaaacg cttgcaccag ttcaggatcg cgatcgaaac cttccggcgt
     5461 tgccacatct tcaacccgat gttcttccca caggccatct gcggcgcgaa aggtacgaat
     5521 acctgattcc gcagaaattc ctgcccctgt cagtacgagt actcttggtt tttccattgc
     5581 ttccggcacc actttatctc tgaaaaaaat acgctgacgc aaacgctcgc gcaggcggcg
     5641 tttattttta cgaaaacgac ttaaccgatg accccgacgc gacagcatag caacctctgt
     5701 tgtttaatcg gttagatgta ggaaggccgc accacgcatt cctcccgcat caccgtggcg
     5761 cgcgcgttca atgcgcggaa cacgagctac aggtaagaga tgacgaggca gcctgtccgc
     5821 cagttgcgtt gtgattgccg ggaaattcga taagccacca ccaatgacga ccaggtcagg
     5881 gtcaacaatg gtcaggatat ttcccagaca aaccgctaat aaatccagat aacgctcaac
     5941 gtgcgccctt gcctgctcat cgccttgatc ataaagcgca ataatttcgg gagcctgcaa
     6001 cggttgatga taatagtgtt gatacagcca cgcaaaaccg cgaccagaca gataattttc
     6061 aatgcagcca tgctgaccac agccgcagcg gcgtaacggg aaatccaacc ccatcatgat
     6121 taacgcatca accggcagac gcatatggcc aaactcgccg gtaatgtagc ttttaccggt
     6181 aatcggtttg ccgttgaaaa tcagcccgcc gccaacgccg gtgccgagaa tcaaccccat
     6241 caccagtgga tattgagtaa attcatcatc ccaggcttct gaaagggcaa aacagttggc
     6301 atcgttatca aggcgtacat cgcgatcaag acgtgcgctc aggtcggcac gcagcggttt
     6361 accgctggca gcggggacat tggcggcata cagcgtacca tcttctgttt ccggcattcc
     6421 cggaataccg atgccgacag aacctttaca gccaaaacgt tggtcagctt cagctaccag
     6481 ctcacacact gcatctaaaa atgcgtcata gctgtcacgc ggtgtcggca cccgcttttc
     6541 ccactgcaac tgccgaccgc tatcaaacac gccaagcgca atttttgttc caccaatatc
     6601 aaacccgtaa tacattgccg ctccttgttt taatgtactg cctttactgg ccgctaagga
     6661 ctcgcgcagg gtcaatatta ctggcgcgcc gcgccggata ccaacttgcc aaaagactca
     6721 gcaacaatgc tgtgaccagt acgtagaaga cgtccagcca gtgcaattcc gatggcagga
     6781 aatcaataaa atagatatcg ctggagagga actgatgacc gatcaacttt tcaatccact
     6841 caataatcgg ggtaagttgc agtgaaacca ccacgccgat aatcacacca cacaggctgc
     6901 cgaacagccc tgccagcaat ccataccaga caaagatggc gcgaattaaa ccatctttcg
     6961 cccccagcgt tcttaatact gcgatatcgc cactcttgtc tttcaccgcc atcactaagg
     7021 tggagacgat gttgaaacag gccacgccaa tcaccagtac catcgccaga tacataatgg
     7081 cgcggatcat ctggatatcg cgatacatat agccgtaagt accaatccaa cttttaatat
     7141 aaacatagct gttggtcact tcacccgcat cgcgtaccag cttattggcg ttgaaaacat
     7201 ccgtcatttt aagggcaata cctgacacgc tggaacccat atcaagatat tgttgggcat
     7261 ccgccagcgg gatcatggca aaactgtgat cgagttgacc actcaactgc aaaataccgg
     7321 caatgtgcaa acgcacacgt tttggctgca tcagtttatg ctcgggattc gagttgggga
     7381 tcatgatcga cacccaatcg ccctgcttca ctttcagcgc atccgccacg cctttgccga
     7441 tgataatttg ctgttcgccc gctttaaaat tgcgccaggc atccccctga acaaacgagg
     7501 gtaatgcgct cagatgctgt tcctgttgtg ggttaacgcc cttcacctgg attgcgcgca
     7561 gattggcacc actttctacc agtccggtga aattgatata cggcgcagcg gcggcaatac
     7621 ccggcacttt ctgcacgtta tccagtgctt cctgccagtt agtccatggc tgatttaccg
     7681 cttcgatttc accatgcggc accaccgcca gaatgcggtt attcagttcg cgttcaaagc
     7741 cgttcatcgc gcttaagccg acgatcaata ccgccacgcc gagggcaata ccaatggtag
     7801 aaatgacaga gatcagcgac accatgccgc cgcgccgccg tccgcgacta aaacgcaggc
     7861 caattaataa cgataaaggc atcgccatta ctccgccccc atcaggctca gttccgccgt
     7921 cagacgacca tcacgcattt ccagttggcg gctcatacgt ttcgccagtt gcaggtcgtg
     7981 agtgaccacc aggaaagcgg tgccctgcaa gcgattcaat tccccaagca actggaagat
     8041 gctgtcggcg ttacgcgcgt caaggttacc ggtaggttca tccgccagta ccaggcgcgg
     8101 gttattcacc agcgcacggg caatcgccac acgctggcgt tcgccgccag aaagttcaga
     8161 tggacggtga ttcgcacgat gctccagccc caccgctttt aacatctcca gcgcacggct
     8221 gttgatttca gcgggctttt tcttgccaat cagcagcggc atagccacgt tttccagggc
     8281 ggtaaaatcc ggcagcagat ggtgaaactg ataaataaag cccagcttct ggttgcgcag
     8341 ctctgctttc gccgccgaag acagtttgct catcggttga ccgttaaaga tcacatcgcc
     8401 ggaggttggc gtatccagcc cgcccaacaa gtgcagcaag gtgcttttac cggaaccaga
     8461 gctaccgacg atcgccatca tttcaccttc gcccacgctg aaactgacat tgtgcagtac
     8521 atcggtttgc acactgcctt cctgatagcg tttgcacagg ttgtcgcatt gcaacaggat
     8581 cttattcata acgtaaagcc tcagcgggtt gtgtggcggc agcgcgccat gaagggtaaa
     8641 gcgtagacag cagcgcgata gccatcgcca ccagcgcaat aacaatgacc tgtaaaggtt
     8701 cgatagccac cggcagcgcc gcgccatcaa gcaggacgcc gattatcggc atcagattat
     8761 ttaactggct ggcaagcagt gcgccaagcg ccgcaccgag gatcgcaccg ataatcccgg
     8821 cgctggcccc ttgcaccata aagaccatca tgatttgtcg cggagttaag ccttgcgttt
     8881 gcaggatcgc tacttcgccc tgcttctcca ttaccatcag ccctagtgag gtaataatgt
     8941 taaacgccgc aacggcgaca atcaggctca gcagtaagcc catcatattt ttttccatgc
     9001 gtacggcctg gaacagctcg cctttacggt cacgccagtc ctgccatttg ctgccttcag
     9061 gcagtttttg ctgacttaaa gagtcaactt tcagcggctc atccagccac aaacgccagc
     9121 cggtaatatt gcctgccgga taacgcatca ggcgtgaggc atcctcaata ttcaccagca
     9181 tttcatagcc atcgacttca ctgttggcgg cgaaagtgcc aatcacattg aacaggcgct
     9241 gacttggaat acgccccatc ggcgtgaact ggctggcaga tggcaccatc acgcggattt
     9301 gatcaccgcg attaacgcct agctgtgagg caagctgttc accgaggatg acattatatt
     9361 tccccggctc gaggtcagtt tgtttcacat tgaccagata cggcgttagt ggatcttttt
     9421 gcgccggatc gatacccagc atcaccccga ccgccacgct gcgcgcgctt tgcagtacca
     9481 catcaccggt agtaataggt gcgacgcgat taacgccgtc cagtttgact gccgtttccg
     9541 ggagttgctg cgggttaaga gagccatgct cagaagagag aattgcctgt ggcatcaggc
     9601 caaggatgtt gttttgcagc tcgcgctcaa agccgttcat cactgacaat actgtgacca
     9661 gcgccatcac cccgagggta atgccgatgg tagaaagcca ggagacgaaa cgaccgaagc
     9721 gatccgctgc acgcccacgc atgtaacgca ggccaataaa tagagcgaca ggttggtaca
     9781 tgaaatccgt ctggttgctg tagcaaagac acgagtatat aagcgaaagc aagatgggta
     9841 aacgtctgaa ctgtaagtga tcgtcagggc taatctgaaa agcccccgta aatcaatcgg
     9901 ctaaattgcc atgttttatc atcaatcttg ccgcctgatc ttaatctgaa aaatcatcca
     9961 atacagacag atacccatta cacctcgggt cataacccgc aggatcaacc gcatgtaaat
    10021 taaaaaagag cgtgttgtcc tgatgaaaca aaaagagcta tggattaacc agatcaaagg
    10081 gttatgtatt tgtctggtgg tgatttatca ctcggtcatt accttttatc cgcatctgac
    10141 cacttttcag catccgttat cggaagtcct gagcaaatgc tggatctatt tcaatcttta
    10201 ccttgcaccc tttcgtatgc cggttttttt ctttatatcc ggctatttga ttcgccgcta
    10261 tatcgacagc gtaccgtggg gaaattgtct cgataaacgc atctggaaca tcttctgggt
    10321 gctggcgctt tggggcgtgg tgcagtggct ggcgttaagt gcgctgaacc agtggctggc
    10381 acctgagcgc gatttaagca atgcctccag tgccgcgtat gccgattcca ccggtgagtt
    10441 cctgcacggg atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta
    10501 tttcgtgata tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact
    10561 gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatccg
    10621 caatttgcct tattacagcc ttggcgcatg gtttggcgca acaataatga cctgtgttaa
    10681 agaggtgccg ttgcgccgcc atctgctgat ggcttctttg ctggctgttc tggcggttgc
    10741 tgcctggttg tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa
    10801 gctgttttat caatacgagc aacgattcgg tatgcgctcc accagcctgc tgaatgtgat
    10861 tggctccaac accattgcta tctacaccac ccatcgcatt ctggttgaaa tattcagctt
    10921 aactctgctt gcgcaaatga acgcagcacg ctggtcgccg caagtcgaac tgacactcct
    10981 gctggtttat ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa
    11041 actttcacgg cgcgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt
    11101 cagttactcc cgctaagcca tcgccccaac cggggcgatt caatttgcta aaccatgtcg
    11161 attacggata atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc
    11221 ctaacgagaa tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag
    11281 cgggtgagca gcgtctgctg ggcgagttaa ccggcgctgc ctgtgcgacg ctggtagcgg
    11341 aaattgccga acgtcacgcc ggtccggtgg tactcattgc accagatatg caaaatgctc
    11401 tgcgtttgca tgatgaaatc agccagttta ccgatcaaat ggtgatgaat ctggcggact
    11461 gggaaactct tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt
    11521 ccacccttta ccagctaccg acgatgcagc gtggcgtact gattgttccg gtgaatacgc
    11581 ttatgcagcg cgtttgccca cacagttttc tccacggtca tgcgctggtg atgaaaaaag
    11641 gtcagcgcct gtcacgagat gcattacgaa cccaactgga cagcgcaggt tatcgccatg
    11701 ttgaccaggt gatggagcac ggcgaatacg ccacgcgcgg cgcgttgcta gatctcttcc
    11761 cgatggggag tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagcctgc
    11821 gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aacctgctgc
    11881 ccgcgcacga attcccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg
    11941 ataccttcga agtgaagcgc gatccagaac atatttacca gcaagtgagt aaaggcacat
    12001 tgcctgccgg gatcgagtac tggcagccac tgttcttcag cgagccgctg ccgccgctgt
    12061 tcagttattt ccctgccaat accttgctgg tgaatactgg cgatctggaa aacagtgccg
    12121 aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgac ccaatgcgcc
    12181 cacttttgcc accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa
    12241 actggccccg ggtgcagcta aaaactgaac atttaccgac aaaagcagcg aatgccaatt
    12301 taggcttcga gaagctgcca gacctggccg ttcaggcaca acaaaaagcg ccgctggatg
    12361 cgctgcgtaa gttcctcgag tctttcgacg gtccggtggt gttctcggta gaaagtgaag
    12421 gtcgccgtga agcgctgggt gaactgctcg cacgaattaa aattgctccg caacgcatta
    12481 tgcgtcttga tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg
    12541 gttttgtcga taaggtgcgt aacctagcgc tgatttgcga aagcgatctg ctcggtgaac
    12601 gtgttgcccg ccgtcgtcag gattctcgcc gcaccatcaa ccccgataca ctgatccgta
    12661 accttgcgga gctgcatatt ggccagccgg tggtccatct ggagcacggc gtcggtcgct
    12721 atgccggaat gaccacgctg gaagcgggcg gcattaccgg cgagtacctg atgctcacct
    12781 atgccaacga cgccaaactg tatgttccgg tgtcgtcact gcatctgatt agccgttacg
    12841 caggtggcgc ggaagaaaac gccccgctgc ataaacttgg cggtgatgcg tggtcacgcg
    12901 cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc ggaattgctg gatatctacg
    12961 cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt
    13021 tctgcgacag cttcccgttt gaaaccacgc cggatcaggc tcaagccatt aatgcggtgc
    13081 ttagcgacat gtgtcagcca ctggcaatgg atcgtctggt gtgcggcgat gttggctttg
    13141 gtaaaacaga agtggcaatg cgcgccgctt tcctggcagt agataaccac aagcaggtag
    13201 cggtgctggt gcctaccacc cttctcgcgc agcagcatta cgacaacttc cgcgaccgtt
    13261 tcgccaactg gccggtccgt atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga
    13321 cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac
    13381 tgctgcaaag tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc
    13441 gcttcggggt gcgtcataaa gagcgcatta aagcgatgcg cgcgaacgtg gatattctga
    13501 cgcttactgc aacgccgatc ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc
    13561 tgtcgattat cgccacgccg cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt
    13621 atgacagcct ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt
    13681 attatctcta caatgatgtg gaaaacattc agaaagccgc cgaacggctg gcagaactgg
    13741 tgccagaagc gcggatcgcc atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg
    13801 tgatgaatga tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa
    13861 ccgggatcga catcccgaca gccaacacca ttatcattga acgggcggat cacttcggtc
    13921 tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc gcatcatcag gcatatgcat
    13981 ggttgctgac accgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga
    14041 ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc
    14101 gcggcgcggg tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct
    14161 cgctgtatat ggagttgctg gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt
    14221 cgctggaaga tctcaccagc cagcaaacag aagtcgagct gcggatgccg tcgctattgc
    14281 cagatgattt catccctgac gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg
    14341 ccaaaacgga aaacgaactg gaagagatca aagtcgagct tatcgatcgc ttcggcctgc
    14401 tgccggatcc ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac
    14461 tggggatcag gaagctggaa ggtaatgaga aaggcggggt gatcgaattt gccgagaaga
    14521 atcacgttaa tccggcctgg ttgattggtt tgctgcaaaa acagccgcag cattaccgcc
    14581 ttgatggtcc gacgcgcctg aaatttattc aggatttgag tgagcggaaa acgcgtatcg
    14641 aatgggtacg ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tggtaaatcg
    14701 ggggcgtttc tgcgccccca tctgcaacat ttacaaattc tttgcacttc cctgcactat
    14761 ccgacactgt caccatccat aattcaggct tatctgttta ttacaataac cttatattta
    14821 ttatggattt ttggtgatga tcaaaacgcg tttttctcgc tggctaactt tttttacgtt
    14881 cgccgctgcc gtggcgctgg cgctaccggc aaaagccaac acctggccgc tgccgccagc
    14941 gggcagtcgt ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct
    15001 ggaagccatc gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaacctcgg
    15061 cgttgatcct tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc aaaccctact
    15121 tccagacgcg ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta
    15181 cccgccgggt aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga
    15241 cacgctgaca ccaacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac
    15301 gccaacggca aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt
    15361 gccggctgga ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg
    15421 cgtttatttg cttcatggta cgaacgccga ttttggcatt ggcatgcggg taagttctgg
    15481 ctgtattcgt ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac
    15541 caaagtgaat atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct
    15601 ggttgaagta catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat
    15661 tacgctgaat agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat
    15721 gcaacatgtg atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag
    15781 cccacaaact ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga
    15841 aaaaatcagc agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt
    15901 ccatggaggg tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca
    15961 tccgcttttt gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg
    16021 attgtaccga ctttttgttg gccttctggc gttgactgaa cttcgaccgc cgcaaagctg
    16081 gcaaatgaca tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata
    16141 gtggccttat gcagatgaat gacgtcgcaa gcaattactt aacgatcgat aactaa
//
LOCUS       DAZFZC010000102        15793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFZC010000102 DAZFZC010000000
VERSION     DAZFZC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.103"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            199..441
                     /gene="yjbE"
                     /locus_tag="ACHKXP_003956"
     CDS             199..441
                     /gene="yjbE"
                     /locus_tag="ACHKXP_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO7648871.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            555..1193
                     /locus_tag="ACHKXP_003957"
     CDS             555..1193
                     /locus_tag="ACHKXP_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO7648872.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGASWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1190..1927
                     /locus_tag="ACHKXP_003958"
     CDS             1190..1927
                     /locus_tag="ACHKXP_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7648873.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1927..4023
                     /locus_tag="ACHKXP_003959"
     CDS             1927..4023
                     /locus_tag="ACHKXP_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7648874.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4070..4348)
                     /locus_tag="ACHKXP_003960"
     CDS             complement(4070..4348)
                     /locus_tag="ACHKXP_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648875.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4349..4492
                     /locus_tag="ACHKXP_003961"
     CDS             4349..4492
                     /locus_tag="ACHKXP_003961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648876.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4618..5028
                     /gene="psiE"
                     /locus_tag="ACHKXP_003962"
     CDS             4618..5028
                     /gene="psiE"
                     /locus_tag="ACHKXP_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO7648877.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5072..6547)
                     /gene="xylE"
                     /locus_tag="ACHKXP_003963"
     CDS             complement(5072..6547)
                     /gene="xylE"
                     /locus_tag="ACHKXP_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO7648878.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6919..7809)
                     /gene="malG"
                     /locus_tag="ACHKXP_003964"
     CDS             complement(6919..7809)
                     /gene="malG"
                     /locus_tag="ACHKXP_003964"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO7648879.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7824..9368)
                     /gene="malF"
                     /locus_tag="ACHKXP_003965"
     CDS             complement(7824..9368)
                     /gene="malF"
                     /locus_tag="ACHKXP_003965"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO7648880.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9522..10712)
                     /gene="malE"
                     /locus_tag="ACHKXP_003966"
     CDS             complement(9522..10712)
                     /gene="malE"
                     /locus_tag="ACHKXP_003966"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO7648881.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11077..12192
                     /gene="malK"
                     /locus_tag="ACHKXP_003967"
     CDS             11077..12192
                     /gene="malK"
                     /locus_tag="ACHKXP_003967"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO7648882.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12264..13604
                     /gene="lamB"
                     /locus_tag="ACHKXP_003968"
     CDS             12264..13604
                     /gene="lamB"
                     /locus_tag="ACHKXP_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO7648883.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAVTRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAYVDEKFSYDINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTLMEIGYDNVKSQRTGDTNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTDKAKGTPNYGKAVPANFEGGSFGRGDSDEWTFGAQMEIWW"
     gene            13847..14767
                     /gene="malM"
                     /locus_tag="ACHKXP_003969"
     CDS             13847..14767
                     /gene="malM"
                     /locus_tag="ACHKXP_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO7648884.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14996..15793
                     /locus_tag="ACHKXP_003970"
                     /pseudo
     CDS             14996..15793
                     /locus_tag="ACHKXP_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3966 a         3961 c         4091 g         3775 t
ORIGIN      
        1 ttgtttttat tttaagttta tgatttttat tgttatttaa atataagttg aaacttatat
       61 ttgatattca ttccaattat cctaaaacgc catcgctaat tccccgcgcc gtaattcgca
      121 tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt
      181 caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt
      241 gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg gtcggcagca
      301 acgtcggttt cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg
      361 ggtgtcgcgc ttgcggcaac cggtggcggt gatggttcta ataccgggac caccacaacc
      421 acgaccacca gtacccagta ataaagtatg tatccccaaa ataattcgag tcattgcatc
      481 tgtggctaga agtatgaagg gattaaccat aaccacactc cggtgtggtt attctgcccc
      541 tctggagaag agtcgtgaag cgacctgcac tcattcttat ctgcctgtta ttacaggcct
      601 gttcagccac gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag
      661 gcgtacagct gacggatgat gatattcaaa atatgcccta cgccagccag tacatgcagc
      721 ttaatggcgg gccgcagtta tttgtggtgc tggccttcgc tgaagacgga caacaaaaat
      781 gggtcactca ggatcaggct actctcgtta cccaacatgg ccgtctggtg aagactctgc
      841 tcggcggcga caacctgatt gaagtgaata accttgctgc cgacccgctg attaaacccg
      901 cacaaatcgt tgatggcgca agctggacgc gcacgatggg ctggaccgag taccagcagg
      961 tacgctacgc caccgcacgc tcagtcttca aatgggatgg caccgatacc gtcaaagtcg
     1021 gcagcgatga aaccccggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct
     1081 ggcataaccg ctattggatc gacagcgaag ggcaaattcg ccagtcggaa cagtatctcg
     1141 gcgcggatta ttttccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca
     1201 aactattgtc gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt
     1261 caaggtgttc agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat
     1321 cgatctggta ggccaaccgc ggctggcaaa cagctggtgg cccggtgcgg tgattagcga
     1381 agagctggca acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga
     1441 acagggcgca gattccagcg ccgacgatgc cgctgcgatt aacgccttac gccagcaaat
     1501 tcaggcgttg aaagtgacgg gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt
     1561 tgccgaacgc ggtaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccgcc
     1621 gtccacggtc acgttgttcg ggcttatcag ccgtcctggc aagcagccat tcactccagg
     1681 tcgcgacgtg gcgagctatc tctctgacca gagcctgctc agcggtgcgg atcgcagcta
     1741 cgcgtgggtg gtttacccgg acggacgcac gcaaaaagcg ccggtggctt actggaacaa
     1801 gcgtcacgta gagccgatgc ccggcagcat tatttatgtt ggcctcgcgg actccgtctg
     1861 gagtgagacg cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc
     1921 tcaataatga aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc
     1981 tacggcgaaa catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga
     2041 ttattacaaa cgcccaccgc gcgtatggca cgggaagggg agttgagtct gaactatcgc
     2101 gataacgatc agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca
     2161 acgctgcgct acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc
     2221 gatcaaacgt ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac
     2281 tggctgccgc aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg
     2341 gaatatcttg ttgccagcaa agcctggggg ccgttcgatt ttacgctcgg cctgggctgg
     2401 ggatatttgg gcaccagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat
     2461 tgctatcgcg ataacagcta caaacaggcg ggatctatcg acggtagcca gatgttccac
     2521 ggtcctgcct cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg
     2581 aaactggagt atgaaggcaa taattatcag caggattttg ccgggaagct ggagcaaaaa
     2641 agtaagttta acgtcggtgc gatttatcgc gttaccgatt gggccgacgt taaccttagc
     2701 tatgaacgtg gcaacacctt tatgtttggc gttacgttgc gcaccaactt taacgatctg
     2761 cgcccgtctt acaacgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt
     2821 ttgcagcatt cggtggtggc gaatcagtta acgctgttga aatacaatgc cggacttgcc
     2881 gatccacaga tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat
     2941 cgtgattcgc gcgaagggat catccgtgcg aatcggatcg tgatgaacga tctgccggat
     3001 gggatcaaaa cgatccgcat tacggaaaat cgccttaaca tgccgcaggt gacgacggaa
     3061 accgatgtcg ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgacg
     3121 ctggcgcaaa aacgcgtcga gccagtggtt ccgcagtcca ccgagcaggg ctggtatatc
     3181 gacaaatcac gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg
     3241 gaaaactttt acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac
     3301 catctgctga ccaccggcag cctgtttgca aatcttgcca acaactacga caagtttaac
     3361 tacactaatc ctccgcagga ctcgcactta ccgcgcgtgc gtacccatgt gcgcgagtat
     3421 gtgcagaacg atgtctatgt gaataacctg caagccaact acttccagca tctgggcaac
     3481 ggcttctacg gtcaggtcta cggtggttat ctcgaaacca tgtttggcgg tgcgggggca
     3541 gaagtgttgt atcgcccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt
     3601 aaacagcgcg actggcgtag tgcaaaagat atgatgaaat tcaccgacta cagcgtgaaa
     3661 accggacatc tgaccgccta ctggacgcca tctttcgctc aggatgtgtt agttaaagcc
     3721 agcgtcgggc agtatctggc aggggataaa ggcggcacgc tggagatcgc caaacgcttt
     3781 gatagcggcg tggtggtggg tggctatgcc acgatcacta atgtttcgaa agaggagtac
     3841 ggcgaagggg acttcaccaa aggcgtgtat gtctcggtac cgttggatct cttctcgtct
     3901 ggcccgacac gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcaa
     3961 caacttgggc gtaagttcca gttgtatgac atgaccagcg accgtagcgt caatttccgc
     4021 taagtcatgg gaaaggtgcc agttttcgca atcactggca ccctcattct taatggcctg
     4081 ttgagccatc aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac
     4141 attctgtctt cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca
     4201 tcattgggct ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac
     4261 cagcataagc aggaagaaca gagactgata acatcaaagc agcaaaataa ggcttcattt
     4321 ttaccacctt tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg
     4381 cattgctggt ggctgggaat cacctgaatg ggtgattttt gaattaccgg ctttggtgcg
     4441 gtttgtcttg ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc
     4501 ggctttggtg gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga
     4561 tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg
     4621 acgtcactct ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat
     4681 cttggcctgc tgtgcctcgg cctgattttg gttgtcttcc tcggcaaaga aacggtgcat
     4741 ctggctgatg tgctgttcgc gccagaacaa accagcaaat atgagctggt agaaggactg
     4801 gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt
     4861 tttcacttcc cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc
     4921 atcgtcgatc ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg
     4981 gtgatcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agtcagcacg
     5041 ccgaaatggc gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct
     5101 tcgtttccgg ttcccagagc gcttccagct cctcaagggt tttacctttg gtttccggga
     5161 caaatttcca cataaacagt gctgccagaa cgcccataca accgtaaatc cagtaggaga
     5221 aaccgttgtg gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg
     5281 agacgaagta gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttaccacgaa
     5341 tagcattcgg gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa
     5401 aggcggcaac atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa
     5461 acgcggtacc gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg
     5521 gcttacgacc aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa
     5581 ctccgacaat aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca
     5641 cttccggcgc gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca
     5701 ttacgccgat tacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc
     5761 catgatccag ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa
     5821 ttttgcgcag gataccttcc gcctgttctt gcttgccgcg cgacatcagc cagcgaggac
     5881 tttctggcac ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa
     5941 acatataacg ccagccgtca gtattcagcc agctggcatc accggaacgg gcaataaaat
     6001 agtttacgca gtaaactaaa agttgcccga aaataatcgc aaactggtta aaagagacca
     6061 gtttcccgcg aatatgagct ggagccagtt ccgcaatata cattggcgag agcattgagg
     6121 ctaaaccaac gccaataccg ccaataatgc gataaataac aaattccggg acataacctg
     6181 ccagataaac aggcactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg
     6241 cagaacctac accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacgac
     6301 cgaagcggtt actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc
     6361 tggccacgca aaaccctaac agggagttgg cagcggattc acttaagttt tgtggagcaa
     6421 caaagacggt attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata
     6481 ataaaccacc taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg
     6541 tattcattca gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat
     6601 tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt
     6661 gatcttaatt gtgataatta tccaaaattg ataaaaacaa tactattgcc gtgactcaga
     6721 gcacgaaaga gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg
     6781 atggaaatat taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt
     6841 ggagcgccga ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac
     6901 attggcagaa caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca
     6961 gcgttgagcc agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc
     7021 aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc tgcatcccca cggccagggt
     7081 gtagctgttt acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat
     7141 aaacgacagg atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg
     7201 gaaggcctgc cacggtgtcg caccatccag cgcagcagct tcttccagcg aactgtcgat
     7261 ggtttcgaaa tagcctttga tggtccagac atgcagcgca atcccaccca gatacgcgaa
     7321 aattacgccg ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa
     7381 caacgcatac aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc
     7441 tttcagcagc gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt
     7501 ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca
     7561 cagcagtacc gggaatggcg gtggcgtaat gcgaccatca gcctgttcaa cgctaaaacc
     7621 taacgccagt ttccagtgat cccaggagat ttgctccggg atcaggctgc cggtcgcaaa
     7681 gtttccctga cgcagcgaga tagcgacgac catcagcagc gggaacataa tcgctgcgat
     7741 aaaaagtagc agtagcaggt gagtaataaa taaacgagct ttttgcgatt tcggttggac
     7801 cattgccatt tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact
     7861 atcgccagcg cacccaccag caggaagatc agcgtggcaa ttgctgccgc cagaccgaag
     7921 tcctgacccc cgccgccttc aaaagcgatg cggtaggtgt agttaacaag caggtcggta
     7981 taaccggctg gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc
     8041 agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta
     8101 atcagcagcg gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct
     8161 gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg
     8221 tacggataac ccagccaggt attgacgata attagcatcg tgcgggcggt ggtcggatcg
     8281 ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ttcaccgaag
     8341 ctctggttaa acaacccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc
     8401 agaatcagca ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacac
     8461 gccagaacca tgccgaccgc caccgttaaa aagacagtga tcagcgagaa caccacggtc
     8521 cagacgaaaa tggcgaggaa cggtttctga atgccttcgt cggtaaagac gcgggtaaag
     8581 tttttccagc cggtggtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg
     8641 tcggcggtaa tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc
     8701 tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac
     8761 tggcgcaggg agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc
     8821 agcgcctgac gattctgggt aatcacgcgc agattcgcgc gttcgccttc gggctgggcg
     8881 gtcgtttctt tcagttgcag tttttgctcg ccgccaaatt taaaagcgtc ggagaggtaa
     8941 tttttgccgg tttcgccgtc gctgagcgcc agttgccact catcgcccgc cgggtaaaga
     9001 ccaaagttat aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgcgcacgt
     9061 tcaaaagtca gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc
     9121 agagggaaga ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg
     9181 taggctttac gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg
     9241 gcgaacaggt attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg
     9301 agcagaccta gcactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg
     9361 acatccatgg ggttcttcct cattccagga cggataaggc tttcacgcct tatccgacaa
     9421 caactgcctg atgcgacgct gacgcgtctt atcaggccta catacgtttc ggttttgtag
     9481 gccggacaag gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc
     9541 tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca
     9601 gtacgcacgg cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc
     9661 tgggcgtttt ccatggtggc ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac
     9721 ttcagcgcta cggcacccag cggtttgtct ttattaaccg cttccagacc ttcgtcagtc
     9781 agcagatagt tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata
     9841 cctgcgctca gcacgccaac gaacggtttg gatggttgac ccttgaaggt cggcagtacc
     9901 gttacaccat aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgatg
     9961 gtcatcgctg tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca
    10021 ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca gacccgcttt cgcgccagcg
    10081 ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt catacttgaa cgcataaccc
    10141 ccgtcagcag caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg
    10201 ctcttacctt tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggc
    10261 gggttcggca gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa
    10321 gcaatcagct tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg
    10381 aacgctttgt ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaagcgg
    10441 tcgtgcgccc agaagataat gtcagggcca tcgccagttg ccgcaacctg tgggaatttc
    10501 tcttccagtt tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc
    10561 ttaccgactt cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta
    10621 ccttcttcga ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat
    10681 gcgaggatgc gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct
    10741 cgtgaaaaca cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt
    10801 gcccctacgc cccaccgtcg ctttgtgtga tctctgttac agaattggcg gtaatgtgga
    10861 gatgcgcaca taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac
    10921 ctcggtttag ttcacagaag ccgtgttctc atcctcccgc ctcctccccc ataaaaaagc
    10981 cagggggtgg aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt
    11041 gctgtcgatg acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat
    11101 gtaacgaaag cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa
    11161 ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg
    11221 attgccgggc ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat
    11281 gacactccgc cagcagaacg cggcgttggt atggtgtttc agtcttacgc gctctatccc
    11341 cacctgtcag tagcagaaaa catgtcattt ggcctgaaac tggctggcgc aaaaaaagag
    11401 gtgattaacc aacgcgttaa ccaggtggcg gaagtgctac aactggcgca tttgctggat
    11461 cgcaaaccga aagcgctctc cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg
    11521 gtggccgagc caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg
    11581 cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt
    11641 tacgtcaccc acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac
    11701 gccggtcgcg tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt
    11761 tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc
    11821 accgcaatcg atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg
    11881 ccagttgaaa gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa
    11941 catctactgc cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag
    12001 caactcggca acgaaactca aatccatatc cagatccctt ccattcgtca aaacctggtg
    12061 taccgccaga acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg
    12121 ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag
    12181 ccgggcgttt aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga
    12241 aaagcaatga ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg
    12301 ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac
    12361 gttccggtat tggttggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg
    12421 ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc
    12481 aggaagtgtg gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg
    12541 tcgcacaaca gaatgactgg gaagctaccg atccggcctt ccgtgaagca aacgtgcagg
    12601 gtaaaaacct gatcgaatgg ctgccaggtt ccaccatctg ggcaggtaag cgcttctacc
    12661 aacgtcatga cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg
    12721 gtctggaaaa catcgatgtt ggcttcggta aactctctct ggcagtaacc cgctcctctg
    12781 aagcaggtgg ttcttcctct ttcgccagca acaatattta tgactatacc aacgaaaccg
    12841 cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat caacccgggc ggcacattag
    12901 aactgggtgt cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg
    12961 catcgaaaga cggttggtta ttcactgctg aacatactca gagtgtcctg aagggcttta
    13021 acaagtttgt tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc
    13081 agggttctgg tgttgcatat gttgacgaaa aattttccta tgacattaac aacaacggtc
    13141 acatgctgcg catcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt
    13201 acgtgggtat gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg
    13261 tcggtattcg cccgatgtat aagtggacgc cgatcatgag taccctgatg gaaatcggct
    13321 acgacaacgt taaatctcaa cgtactggtg ataccaacaa ccagtacaaa atcacacttg
    13381 ctcaacaatg gcaggctggc gacagcatct ggtcgcgtcc ggctattcgt gtgtttgcca
    13441 cctacgccaa gtgggatgag aaatggggtt acgattacac cgacaaagct aaaggtactc
    13501 cgaactacgg caaagccgtt ccagctaact tcgaaggcgg cagcttcggt cgtggcgaca
    13561 gcgacgagtg gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc
    13621 ggataaggtg cttgcgccgc atccggcatc caacgcctga tgcgacgctt gcgcgtctta
    13681 tcaggcctac aacggctgtc aaatgtaggc cggataaggc gtttacgccg catccggcat
    13741 aaaaacaggt tgtcattatc tgaaaggggc gaaagcccct ctgattatcg ggtttagcgc
    13801 gctattgcct ggctaccgct gagctccaga ttttgaggtg aaaacaatga aaatgaataa
    13861 aagtctcatc gccctctgtt tatcagcagg gttactggca agcgcgcctg gaatcagcct
    13921 tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg ccagccattc catctgccgc
    13981 gctgcaacaa ctcacctgga caccggtcga tcaatctaaa actcagacca cccaactggc
    14041 gactggcggc caacaactga acgttcccgg catcagtggt ccggttgctg cgtacagcgt
    14101 cccggcaaac attggcgaac tgaccctgac gctgaccagc gaagtgaaca aacaaaccag
    14161 cgtatttgcg ccgaacgtgc tgattcttga tcagaatatg accccatcag ccttcttccc
    14221 cagcagttat ttcacctacc aggaaccagg cgtgatgagt gcagatcggc tggaaggcgt
    14281 tatgcgcctg acaccggcat tggggcagca aaaactttat gttctggtct ttaccacgga
    14341 aaaagatctc cagcagacga cccaactgct cgacccggct aaagcctatg ccaaaggcgt
    14401 tggtaactcg atcccggata ttcccgatcc ggttgctcgt cataccaccg atggcttact
    14461 gaaactgaaa gtcaaaacga actccagctc cagcgtgttg gtcgggcctt tatttggttc
    14521 ttccgctcca gctccggtta cggtaggcaa cacggcggca ccagctgtgg ctgcacccgc
    14581 tccggcaccg gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt attttaatac
    14641 cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac tgcttgatga
    14701 agctgaacgc ctgggatcga catctgcccg ttccaccttt atcagcagtg taaaaggcaa
    14761 ggggtaatta cgccccacag tgctgatttt gcaacaactg gtgcgtctcc tggcgcacct
    14821 ttttttatgc ttcattccag ggatatgagc gattttttat agtacttcat ttcttcttca
    14881 ctaagaatgt gcattatctc aatgccttat cagagattct tttcctttcg ccggtagtgt
    14941 ctggacgctc aggctacttt ttccagttat ttcatttctg taatgcagag gttttatgtt
    15001 aggtcatatc tcaaagtttg acggcaataa ctctttgata aaacatggtg tggtgcaagg
    15061 aaataatata gtcgatcttg atttactacg taattttaat ggggtgccag ggttaaatcg
    15121 agaaaacttt atttatatca gcaatatttt ttcaaatata aaacaacgga acgaaaaaat
    15181 catgcaataa atatgtttcg tgaagtctca atcagtaatg atactataag tgtaaaattc
    15241 tacagaaatg aaaaaaattg aatgcgcttg caattttctg atggataaag atgcgcaggg
    15301 gtatatcgac ctgtctgatt tggatttaac aagttgtcat tttaaaggtg acgttatatc
    15361 gaaggtgtct tttttatcat caaatctaca acatgtaaca ttcgaatgta aagaaattgg
    15421 ggattgcaat tttactactg caatagttga taatgtcata tttagatgtc gacgtttaca
    15481 caatgtgatt tttatcaaag cgagtggtga atgtgtcgat ttcagcaaaa atattcttga
    15541 tacagttgac ttctcgcaga gtcaacttgg tcatagtaat tttcgcgaat gtcagattag
    15601 aaattcaaac ttcgataatt gttatcttta cgcttcgcac ttcaccagag cagagtttct
    15661 gtctgccaaa gaaatatcat ttattaaatc gaatttgaca gctgttatgt ttgattatgt
    15721 gcgaatgtcg acagggaatt ttaaagattg cattacagaa caattggaat taactattga
    15781 ttattcagat ata
//
LOCUS       DAZFZC010000103        15630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFZC010000103 DAZFZC010000000
VERSION     DAZFZC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.104"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(40..1290)
                     /gene="icd"
                     /locus_tag="ACHKXP_003971"
     CDS             complement(40..1290)
                     /gene="icd"
                     /locus_tag="ACHKXP_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO7648885.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1462..2115
                     /gene="rluE"
                     /locus_tag="ACHKXP_003972"
     CDS             1462..2115
                     /gene="rluE"
                     /locus_tag="ACHKXP_003972"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO7648886.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGFPTQDAIEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2125..2586
                     /gene="nudJ"
                     /locus_tag="ACHKXP_003973"
     CDS             2125..2586
                     /gene="nudJ"
                     /locus_tag="ACHKXP_003973"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO7648887.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICSTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2640..3746
                     /gene="mnmA"
                     /locus_tag="ACHKXP_003974"
                     /gene_synonym="trmU"
     CDS             2640..3746
                     /gene="mnmA"
                     /locus_tag="ACHKXP_003974"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO7648888.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3782..4423
                     /gene="hflD"
                     /locus_tag="ACHKXP_003975"
     CDS             3782..4423
                     /gene="hflD"
                     /locus_tag="ACHKXP_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO7648889.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4427..5797
                     /gene="purB"
                     /locus_tag="ACHKXP_003976"
     CDS             4427..5797
                     /gene="purB"
                     /locus_tag="ACHKXP_003976"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO7648890.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5965..6636
                     /gene="phoP"
                     /locus_tag="ACHKXP_003977"
     CDS             5965..6636
                     /gene="phoP"
                     /locus_tag="ACHKXP_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO7648891.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6636..8096
                     /gene="phoQ"
                     /locus_tag="ACHKXP_003978"
     CDS             6636..8096
                     /gene="phoQ"
                     /locus_tag="ACHKXP_003978"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO7648892.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGDSMLGGARMEVIFGRQHSTPKDE"
     gene            8172..9293
                     /gene="roxA"
                     /locus_tag="ACHKXP_003979"
                     /gene_synonym="ycfD"
     CDS             8172..9293
                     /gene="roxA"
                     /locus_tag="ACHKXP_003979"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO7648893.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9342..10568)
                     /gene="pepT"
                     /locus_tag="ACHKXP_003980"
     CDS             complement(9342..10568)
                     /gene="pepT"
                     /locus_tag="ACHKXP_003980"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO7648894.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10818..11954
                     /gene="potA"
                     /locus_tag="ACHKXP_003981"
     CDS             10818..11954
                     /gene="potA"
                     /locus_tag="ACHKXP_003981"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO7648895.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11938..12801
                     /gene="potB"
                     /locus_tag="ACHKXP_003982"
     CDS             11938..12801
                     /gene="potB"
                     /locus_tag="ACHKXP_003982"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO7648896.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            12966..13049
                     /locus_tag="ACHKXP_003983"
                     /pseudo
     CDS             12966..13049
                     /locus_tag="ACHKXP_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(13075..13665)
                     /locus_tag="ACHKXP_003984"
     CDS             complement(13075..13665)
                     /locus_tag="ACHKXP_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFO7648897.1"
                     /translation="MPMNTTGTSFSSFGISCHRENSFRNSFRGKNDEVIKCSMGERTI
                     RFSVRKFSGNILDTVSRQSTKDINGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQEYQLTLDVKAAQSSINHVIMGNASFGKKIDALCDSMSRDVKNRTADSI
                     ANLLADKFYQKHIEPDIDIVKLRNEIPDYLRCAIQA"
     gene            13848..14498
                     /locus_tag="ACHKXP_003985"
     CDS             13848..14498
                     /locus_tag="ACHKXP_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO7648898.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            14573..>15630
                     /locus_tag="ACHKXP_003986"
     CDS             14573..>15630
                     /locus_tag="ACHKXP_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648899.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNARNQNKFPIDVKETVIKLQGQKFTCIEDFHKKYNETRLKANTNIQQKQI
                     ADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISPDDTDKITGRKFS
                     ELGEYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRLYSDEVLQRNGTLIPTP
                     LHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSP
                     RNYGLHHNIKTWDPLVRN"
BASE COUNT         4084 a         3842 c         3709 g         3995 t
ORIGIN      
        1 gtattatacg cccgttgcaa tattaaacac atgtagtgat tacatgttct tgatgatcgc
       61 atcaccaaat tctgaacatt tcagcagttt agcgccttcc atcagacgct cgaagtcata
      121 ggttacggtc ttcgcattga ttgcgccttc cataccttta acaatcaggt ctgcggcttc
      181 agtccagccc atatggcgta acatcatctc agcggagaga ataatagagc cagggtttac
      241 tttgtcctga ccggcgtatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
      301 gtcaccgatg tttgctccag gggcgatacc gataccgcca acctgcgctg ccagagcgtc
      361 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
      421 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
      481 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
      541 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
      601 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
      661 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
      721 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
      781 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
      841 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
      901 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
      961 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     1021 atcaagagtt tcagcaggca gccagacgtc ctgaccataa acctgtgtgg atttttcacc
     1081 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     1141 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     1201 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     1261 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     1321 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     1381 tttttgttaa tgatttgtaa ttggcttgtc aatactaccc tattgtttgc cgtcatgaaa
     1441 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     1501 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac caggcgtaaa
     1561 cctgaaaacc agcccacgcg tgtgatcctc ttcaataaac cctacgatgt tcttccgcag
     1621 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     1681 gcagcaggtc gtcttgaccg cgatagcgaa gggttactgg tgctgaccaa taacggcgcg
     1741 ttgcaggcgc gtttaaccca gccgggtaaa cgcaccggaa aaatctatta tgtgcaggtg
     1801 gaaggttttc ccacgcaaga cgcaattgaa gccttgcgca atggcgtaac cttaaatgat
     1861 ggccctaccc tgcccgcagg cgcggagctg gttgacgaac ccgcgtggtt atggccgcgg
     1921 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     1981 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggcttccc cacgctgcga
     2041 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     2101 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     2161 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     2221 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     2281 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     2341 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     2401 tcgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     2461 ttacaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagc
     2521 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     2581 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     2641 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     2701 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     2761 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     2821 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     2881 acgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     2941 tgtgcaacaa agagatcaaa tttaaagcct tcctcgaatt tgccgccgaa gatttaggtg
     3001 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     3061 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     3121 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     3181 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     3241 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggcgcaa ccgggcaaaa
     3301 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     3361 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg taccgaagaa ccgtggtatg
     3421 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     3481 ggctgatgtc tgttgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     3541 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     3601 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     3661 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     3721 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     3781 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     3841 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     3901 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     3961 caacctgcgc gtcgggctgg aaaccttgct cggcgtgctc aatgccagca gtcgccaggg
     4021 cttaaacgct gagttaaccc gctacacact cagcctgatg gtgcttgagc gcaaactctc
     4081 ctcagcgaaa ggtgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     4141 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     4201 tgtgattagc ccgcttggcc cgcgcattca ggtcaccggt tcccctgctg tactgcaaag
     4261 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     4321 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     4381 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     4441 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     4501 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     4561 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     4621 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     4681 cgagcgtact actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     4741 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     4801 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     4861 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     4921 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     4981 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     5041 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     5101 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     5161 tacccagatt gaaccgcacg actacattgc cgaactgttt gattgcgttg cgcgcttcaa
     5221 caccattctg atcgactttg accgtgacgt ctggggttat atcgccctta accacttcaa
     5281 acagaaaacc atcgccggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     5341 cgacttcgag aactccgaag ggaacctggg cctttccaac gcggtattgc agcatctggc
     5401 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     5461 cctcggcgta ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     5521 caaactggaa gtgaaccgtg accatctgct ggatgaactg gatcacaact gggaagtgct
     5581 ggcagaacca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaaatt
     5641 gaaagagctg actcgcggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     5701 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg ctaactatat
     5761 tggtcgagct atcacgatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     5821 atgctgtccg gcctgcttat taagattatc cactttttct tttttcactt tacctcccct
     5881 ccccgctggt ttatttaatg tttaccccca taaacacata atcgcgttac actattttaa
     5941 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     6001 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga cgcagaagat
     6061 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     6121 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
     6181 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
     6241 gccggtgctg atgattatgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg
     6301 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct ccccccgttt
     6361 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
     6421 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
     6481 tcgttaatgc tacaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
     6541 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattaccacc
     6601 gttcgcggcc agggctatct gttcgaattg cgctgatgaa aaaattactg cgtctttttt
     6661 tcccgctctc gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt
     6721 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
     6781 ttcggctgtt acgtggcgaa agcaatctgt tctataccct tgcgaagtgg gaaaacaata
     6841 agttgcatgt cgagttacca gaaaatatcg acaagcaaag ccccaccatg acgctaattt
     6901 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
     6961 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
     7021 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
     7081 atgacgacga cgcggagatg acccactcgg tggcggtaaa cgtctacccg gcaacatcgc
     7141 gaatgccaaa gttaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
     7201 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
     7261 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
     7321 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
     7381 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
     7441 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
     7501 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
     7561 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
     7621 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
     7681 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
     7741 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
     7801 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
     7861 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
     7921 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtgggg ctggcggtag
     7981 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagatagc atgcttggcg
     8041 gtgcgcggat ggaggtgatt tttggtcgcc agcattctac gccgaaagat gaataaatat
     8101 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
     8161 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
     8221 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
     8281 cagacgagtt ggcgggtctg gcgatggaaa gcgaagtcga cagtcgactg gtcagtcacc
     8341 aggatggcaa atggcaggtc agccacggtc cgttcgaaag ctacgatcat ctcggtgaaa
     8401 ctaactggtc attgttagtg caagcagtaa atcactggca tgagccgacc gccgcgctga
     8461 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatc tctttttctg
     8521 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
     8581 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacacc
     8641 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggcg
     8701 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
     8761 actattccgt gggttttcgc gcgccaaata cgcgggaatt aattagcgga tttgccgatt
     8821 atgtgctgca acgtgaactg ggcggcaact actacagcga tccggatgtt ccacctcgcg
     8881 ctcatcctgc ggacgttctg ccgcaagaaa tggataaact gcgtgagatg atgctcgaat
     8941 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
     9001 atgaactgga tatcgcgccg ccggaaccgc cttatcagcc agatgaaatc tacgatgcgc
     9061 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
     9121 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
     9181 ccagcaacat tgcgctgacc gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
     9241 tcgcgatgct cgcggcgctg gtcaatagcg gatactggtt cttcgaaggg taagtttgga
     9301 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
     9361 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
     9421 tcatgcttac catgatagtt gtaaccgcca gtgaacaggt tcgggcacgg taatcccata
     9481 aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt tcagttccgg ttcaatatcg
     9541 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctcg
     9601 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
     9661 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
     9721 tcgaaatcac ggatgatgta gtgcatatcg gctcggtcaa cggtgccttt catgctcgcc
     9781 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
     9841 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
     9901 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
     9961 agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca
    10021 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    10081 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaataccg
    10141 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    10201 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    10261 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    10321 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    10381 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    10441 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    10501 ctcacccctg cttttgattg ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt
    10561 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    10621 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    10681 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    10741 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    10801 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    10861 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    10921 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    10981 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    11041 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    11101 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    11161 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    11221 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    11281 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    11341 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    11401 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    11461 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    11521 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    11581 acgccactgt catcgaacgt ctagacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    11641 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    11701 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    11761 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    11821 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    11881 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    11941 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    12001 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    12061 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    12121 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    12181 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    12241 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    12301 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    12361 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    12421 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    12481 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    12541 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    12601 ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    12661 gtacagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    12721 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataagaag
    12781 gcaacagaaa tagacgatta gtgttttata ttcaaaaggt taatagcaaa tttggcatgc
    12841 tttttactgt cgagtaaact gcatgcctct atccataaaa tcaaacagtt gcaatctaat
    12901 tttggagaac gttttttctt caccctttta gcaaatcgct ataaaaaaca acaaatggta
    12961 taaaaatgaa caatcctaat ccatgcatga cgtatggcgc ctgttgtgca tttttccgcg
    13021 tctcttttta ccagagcaaa accaatgata ctgacctagt cgtctcccag agacttatgc
    13081 ctgtatagca cacctcaaat aatctgggat ctcatttcgt agttttacaa tatcaatatc
    13141 cggctcgata tgtttctggt aaaacttgtc tgcaagcaag tttgctatgc tatctgccgt
    13201 acgatttttt acatcccggc tcatactatc acaaagagca tctattttct tgccaaaaga
    13261 agcattcccc attatcacat gattaataga actttgtgct gccttaacat caagagttag
    13321 ttgatattcc tgactcacaa gagaaaagac cctttgcctc tcttcagtgg aaatttttgc
    13381 atttttttga aaacagtaat tatcaatctc ctggttgatc acccttgagg gatatactat
    13441 ccgttcatct tttatccacc cattaatatc tttagtactc tgcctactca ctgtatccag
    13501 tatattgccg ctaaatttgc gaacagaaaa gcgaatcgtc cgttctccca ttgaacattt
    13561 tatgacctca tcattcttcc ctcggaaaga gttcctgaag gagttttctc tatggcaact
    13621 tataccaaaa gaactgaaag acgtacctgt agtattcatc ggcattataa ttccttttta
    13681 taaaaaagtg tttacaatat tgcatacttg tgtttattgt agccagaaaa aacatgcatg
    13741 caataatatt ctattaaaaa ttgtattgtt ttattcacat atcatgagcg gtatgttgac
    13801 tcttgttacc aatatagttc acaaaatggg ttgaaaagga taataatatg ccagtcatat
    13861 taaatttttc taatggaagt gtattgccgg aaaatgagct ggaggcttta cgacatattg
    13921 caagaagcaa tcagaatgac actattacta taggaggccg taatatgagg cttcattata
    13981 tccagtttat ggatggtttt agtgtagagc ctattcctgg tggactttgg gaccatcttg
    14041 gggcaagaga gtctcatcat ctcgcagaca gcctggcaag acaacttaac ggaggtaaca
    14101 cttttctgca ggcatacagc ttatatctcg agcagaggca agctgccccg cttgtgcagg
    14161 aaagcgtcat aaaaacacta ctagatcgaa taaattcgaa tgcatttccc gttagtttac
    14221 aagacttttc ctgtactgag gagcatctta attgtccgat aacgctacat attcctgaga
    14281 caggtgtttt tgtcagaaat gctcgaaatt cagaaatatg tgcattatat gatcaggaag
    14341 cattgactga acttatcctg cgtaacgctc tccaccccct cagccgtgac ccctttgctc
    14401 cagaaatgat tataagcaaa gacaagtgtc attttaatat aacaaaacaa tgtttttacg
    14461 cattacccat ataccccctt caacaaaaca gtatttaaaa ataaaaacac aatataagaa
    14521 gcatacaata agttaatcac catataacaa acatatagcg aggagattat ttatgcccaa
    14581 aatatcatca gttgtatcat catgttacca tctgttcagt gaacatcaac aactttcaaa
    14641 tgaaacaaca atgacgaact ccgtctccag aagaattgtt cataaagaat atggtatatc
    14701 tttaaaatcc gttcctgtat ggttggctac agctaaaact ccccttgctc tactcaatgg
    14761 cagacataca agaagtcact catttattat tgcagggact ccaggaatgg gaagcaggag
    14821 cggagcccaa tactatgcca taaacagtga tgataaacgc tcccgcatag acattgactc
    14881 tttattttta aaaaagttaa ataatgcgcg aaatcaaaat aaatttccaa tcgatgtaaa
    14941 agaaacggtt ataaaactac aaggccaaaa atttacatgc attgaagatt ttcataaaaa
    15001 gtataatgaa actaggctaa aggccaatac caatatccaa caaaaacaaa ttgcagatga
    15061 agtaaaatca cttacatatt tgattccttc agaaaaaaaa gagatgtgga tatataaaaa
    15121 caatggaaaa gataatgcaa aaccaaactt aggagagcga gacgtaagaa tgttcgaaaa
    15181 tattagtcct gatgatacag ataagataac aggaaggaaa ttttcagagt taggtgagta
    15241 tctttattca ggaaatgtaa taaaactcag tcagttatca attcgttact tacccaatat
    15301 caactcaatc tcattaatag agacaaaaca gagtttgttg ctacatcgat tatattcaga
    15361 tgaagtactt cagagaaatg gaacacttat cccgacacca ctacatgaag aaaaatcaat
    15421 tccagctgac aatataaaaa caatgctcaa caacataccg acttacaaaa tgttaccgcc
    15481 attcacagaa acacaaggta attgttcttc tggcgcagcc acgtttttac gcaaatcagg
    15541 cgccgaagaa aaagatattc ttgcatgtag cccccgaaat tatgggctgc atcataacat
    15601 aaaaacatgg gaccccttgg ttagaaatta
//
LOCUS       DAZFZC010000104        15514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFZC010000104 DAZFZC010000000
VERSION     DAZFZC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.105"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(464..2665)
                     /gene="iutA"
                     /locus_tag="ACHKXP_003987"
     CDS             complement(464..2665)
                     /gene="iutA"
                     /locus_tag="ACHKXP_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO7648900.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTADLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(2691..4024)
                     /gene="iucD"
                     /locus_tag="ACHKXP_003988"
                     /pseudo
     CDS             complement(2691..4024)
                     /gene="iucD"
                     /locus_tag="ACHKXP_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            complement(4021..5763)
                     /gene="iucC"
                     /locus_tag="ACHKXP_003989"
     CDS             complement(4021..5763)
                     /gene="iucC"
                     /locus_tag="ACHKXP_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO7648901.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            complement(5763..6710)
                     /gene="iucB"
                     /locus_tag="ACHKXP_003990"
     CDS             complement(5763..6710)
                     /gene="iucB"
                     /locus_tag="ACHKXP_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO7648902.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRIDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            complement(6711..8435)
                     /gene="iucA"
                     /locus_tag="ACHKXP_003991"
     CDS             complement(6711..8435)
                     /gene="iucA"
                     /locus_tag="ACHKXP_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO7648903.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            8571..9764
                     /locus_tag="ACHKXP_003992"
     CDS             8571..9764
                     /locus_tag="ACHKXP_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648904.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            complement(10144..10524)
                     /gene="crcB"
                     /locus_tag="ACHKXP_003993"
     CDS             complement(10144..10524)
                     /gene="crcB"
                     /locus_tag="ACHKXP_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO7648905.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(10865..11278)
                     /locus_tag="ACHKXP_003994"
                     /pseudo
     CDS             complement(10865..11278)
                     /locus_tag="ACHKXP_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            11276..11533
                     /locus_tag="ACHKXP_003995"
                     /pseudo
     CDS             11276..11533
                     /locus_tag="ACHKXP_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(11767..12624)
                     /gene="sitD"
                     /locus_tag="ACHKXP_003996"
     CDS             complement(11767..12624)
                     /gene="sitD"
                     /locus_tag="ACHKXP_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFO7648906.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(12621..13478)
                     /gene="sitC"
                     /locus_tag="ACHKXP_003997"
     CDS             complement(12621..13478)
                     /gene="sitC"
                     /locus_tag="ACHKXP_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFO7648907.1"
                     /translation="MNALLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(13475..14302)
                     /gene="sitB"
                     /locus_tag="ACHKXP_003998"
     CDS             complement(13475..14302)
                     /gene="sitB"
                     /locus_tag="ACHKXP_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFO7648908.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(14302..15216)
                     /gene="sitA"
                     /locus_tag="ACHKXP_003999"
     CDS             complement(14302..15216)
                     /gene="sitA"
                     /locus_tag="ACHKXP_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO7648909.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANSQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         3916 a         3964 c         3901 g         3733 t
ORIGIN      
        1 gtattggtaa tatgacaata ttttagagag tatattttat tcattataaa atgccccgta
       61 atatttatct cacaatgatt atgattagtg gatacgcccc tacatttcca gacagctgtt
      121 atcacttaat ctattactgg cccgcagccg tagatattct agccacggtg gtaacgagta
      181 tccggaagag attccactta cactctttca atcctattgc aaaagctgat ttactcaaca
      241 ggattaaaag agggagtttg atacaattta tttcactttt tgttagaata ctaattcttt
      301 tcttgtattt tttgaggaaa tgagcacgga acacgacata atctgtagtc agtaaagagt
      361 ctatcctccc tgactacaga ttatgtcgtg tattatatga tatcaacttg gctgtcagcg
      421 gccatgttga tatcagcgta cctttgttgt aaaggaatac cggtcagaac agcacagagt
      481 agttcagacc aaaggttcgg cccctgcctt tgtagtcgta cagtgacgct gggccgtaac
      541 ccgggctgta gtacagtggt gcacgctgcc cccagacagt ggtgtagtca cggtcgaaga
      601 ggttttcaat gctgaagctg agtgtaccca ccggaagctg ataactgccg agcagatccg
      661 cggtggtata gccatcgacc ttgtagccct gcgcgtcgct cacgtcaaag gaggtggtgc
      721 tctgcacgcg cagactccac gggtccggtg cccagccaat gtaggctgtc gcttttgatg
      781 ggcttgctgt cttcacatcg tatttctgcc aggtaccgtt cactttcgac tcagttttca
      841 gcacgttgaa gttcactccg gtactccagt cagtatcagg aatcaggtag tccaccgcac
      901 cttccacgcc gtaaatacgg cgtttgtcgt ccaccacgct gatggtcaga tctttattcg
      961 ccaccacgct cttatcagaa atcgaatagt aggccgcgat ttgggtacgc agattattgc
     1021 cagtaaagcg ccagcccagc tcataagaat cgactttcac gccttccagc ttgctgtcgc
     1081 tgacgttcac actctttgtt agaggaagat ggccgttcac tgcagcacca tagatgccgc
     1141 gaccatagta tttacccggg tccggcagct ccacgccctg ggagaagttg agccatgcct
     1201 gctggcgttc ggtgatgtgc atcagcagac cggcgttgaa caggaagttg tcgtaatcga
     1261 ctgagccgcc aggaatggcg tcggcggatg tagccttccc ggcggcaatc tgccgttgct
     1321 gcgcgtagcc gatgaaatca tcaatcttgt tttcagtgta ctgatagcgt acgccaccgt
     1381 tgagggtaaa gagattattg atgtcatagc ctgattgcag gaaggccgcc aggttggtga
     1441 tgtcatacga cggatagcgc ccggtggtgt aaatcttctt gttgttcagc cctccggaag
     1501 cgcttgcctg agccaggtcg aagaacatct ggttggaggt aaagcgctca tgatcagcat
     1561 ccagccccca ggtgatttgc cagccgtcca tcggtttgct gttcagagtc agtttcatgc
     1621 cgtactggtc ggtgtcctgc tgtgacgaag agaaagccgt cacctgtttg ttcgcattta
     1681 ccgtcgggaa cgggtagaat cgcaacgact catcgcggta gtaaacctga ccgaccagct
     1741 cctgtcccag aaaagcgctg tcagagtact gcaggctgat caaatgccgc tcagtgccgg
     1801 gaatacggtc ggaattcagc ccgttactga cgaatggcgt gctggtccct ctgatggcag
     1861 agaagccttt cccgagatta agcccgtaat cgtcgtcgcc ctggctttta tagtactgtg
     1921 tgatcaactg aagctgccgg gattcatcga tgttcagcgt accagttccc atgatgtcca
     1981 gccgatcgga gtactgcagg ccggtctggg tgttatcaag caaggtggca tcgccgttac
     2041 cgtcaaacca gccgccaaat ttctgatatg ccacggaaag acgtccggag atatgctcat
     2101 ttccgccgga gacagctccg gcaatgcgtt catcgtgatc tttactgctg ctaaagccac
     2161 ttttggtgcc agcctcaaac tccatcatgg tttccggctg gccttttttg gtcacgatgt
     2221 tgatcaggcc accggtactg ccgccgccgt acagggacgt cgcaccggag atcacttcaa
     2281 tatggtcgat attaaaagga tctatagagt ccagttgtcg gctgtcggta cgtgaagagt
     2341 tgagacgcac gccgtcaacc agcacgacca gcgggcggcc acgcacattc ataccgtagt
     2401 tggtgcggct ccggctgctg acgtcaaggc cagggatcag ctgagccagt gcgtctttaa
     2461 gctctttgcc gccctgaatc tgctgttcca gttcggcgtt ttcgataacc caggtggttt
     2521 gcgccatctc cgctacggtg cgattgctgc ggttggcaga caccacgaac gtttcatcat
     2581 cggtttgttg agcgactgct ggcgccatca tggtaagaag cagtgggttg agagcccaaa
     2641 gcgtatactt tttgcttatc atcattattc ctccatacgg gcggaaaaaa tcagcccaaa
     2701 gatgctgtgt agcgagttaa agaagcagcc tccggctgcg ttttccctag gtgccgctgc
     2761 gccactgaat cagggcgggc ggcatactga gatcgaataa atcacgtccc attacgcgat
     2821 taagaatacg tgcagatctc catgccatca ggctgagctg gggttcggcg atgccatggg
     2881 tttgcatact ggcgttgacc acgaagatgt tgttctcttt cgggccactc cattccagag
     2941 tgaagtcatc acgcacttta aaggtgttct tatcgtgcat ggtgatcagg ggcatcagtg
     3001 agggaagtat ttgtggcaac gcagaacggt aacctgtggc gaaaatcacc acatcacttt
     3061 ccaggctctc cctgccctga tccagatgat gctccatcag caacttccag ccaggaccac
     3121 tactttccag agttgttacc gagcggctgg gtagcagacg gatatttctt ggttttctca
     3181 gaacttcaaa acggtggtac aactcacgat aaatggtcag taaagaatcg gcagtgatgc
     3241 catccgatgt cattttctgc tcatccagta actgatggcg aatatcttcc tccagtccgg
     3301 agaagcctga aatatattca ggtgtaaaat actcatcagc aaaagcagcc tcatccagtg
     3361 cgttaaaatt attacgccgg gagacccagt ttatttccgc cgcttctccc cattccccgc
     3421 gtaatgcatt aaggaacagg tctgcaccac tctgtcctcc accaaccacg gttatccgtt
     3481 ttccactaag gtccggccga cgaagattca tttcactggc atggaaacag gattgtgtca
     3541 tatgcttcac acagggtggt aaataaggtt gttttcctgt accaaggcag atattgcggg
     3601 caaaatattc tccctggctg gtttgcacca gaaacaatcg acgtttttta tcgaaatcaa
     3661 tgttttcaac ggtatgactg aaatacaggt tattcatatc ttcagcagcc cagcggaggt
     3721 agtcagaaaa ctcttcacgg gatactgtac gtagtctgct tgtaaggaag cgatagaact
     3781 ttttgtgctt caccagatag ttaacaaaac tgtagggatt tgtaggtgca acagcactga
     3841 ccagatcttt cagaaagacg gtctgcatat gacaatccgg taccagcata cccggatgcc
     3901 aggaaaaatg aggatgttca tcaaagaaga gacagtccag ttcttcgatc tgatgtgaca
     3961 acgcagcaat gctgagatta aatggccctg tccctacacc aataaaatcg acactttttt
     4021 tcatgattca tattcctgag ttaccagcca cagcggattt tgcagatcct caaggtaatt
     4081 cggcagcatg cggctgccgc catccagatc aggccaggtc agttttaccg ggttcagcac
     4141 cacgcgaatg atttgtggcc tgaaaagtga gaaaagcgca aaacgctctg acatttgtgg
     4201 atgttttttc atgtaatcac tcaacactgc tgccagcagt tgataaaatc gcctttcagg
     4261 tacgccaaga cgaaccatca gtggcgaaat aaaacgcagt actgtcacga agtgacccgt
     4321 ctgcaaatca tggattaagt agtccgcact caggcgggat gtaacatcac gaacctcctg
     4381 aggcaaagag tccatttcgg ggaactcttc tttcaccagt cgcatatcgc cctggaagtc
     4441 tttcagcaga acacgctgtg gaaccccctc tttcatggcg agagttatat tttgtccatg
     4501 tgcaataagc gcgacaccgt agcggcaaag caggtgatac agaggaacca cgaccacccg
     4561 gaacagttgc gtaagccagg tttcagcgtc cagcccggag cggtctatat atgcgcctgc
     4621 cagcggctga ttgttttcgt cgcactccat cagtgttgcc atcagaaccg gactttcatc
     4681 cggtttcagc cagcggcacg gattctcccg ccagataaca ccaagcattt cctggtagcg
     4741 atagggagcc cgggcaagcg cggcatagcc ttcatgggag acatagcctg cagccggttc
     4801 accaagtatc actgcgccgc tttgcactag ggtggcgtcg gtcgcaaaaa cctgttgtag
     4861 ccagcgtgaa gccagtggtc cggcagcgat gtatctgcca gggatccccc ggtagcatga
     4921 ggtgttgtag atggtcagcg gcagcttgat atccagcccg ccccggcgac tggcgttggt
     4981 cagggtacgc agcgactgct gggcgagcca ctggtcgcca aactcgccga gagacaccat
     5041 cctgccttcg gcaaaatcag cgatgaagtc ggtagcgatt ttttgctgcc actgccacgg
     5101 atgtaccggc agcggcagcc agttatgatc cagtccgttt tcctgccaga cctgactgaa
     5161 gcgggcaaac tcctgcggat ccattgcggc cgtcaacaac tgatgaatat ccatctcgtt
     5221 atcacagcgc cagatcatat gttcacgttt taccgccagc cagtgcagtc tgaaggtgtt
     5281 ggcatactct ggcgcatatc gttccagcgc ctctttaccc cagccacggc gacctttatt
     5341 aaaaacgaat ttaggatgac cgctcagcag gcattgcagg cggtcggcat taagattaat
     5401 caggtcactg gcgctcagcc cgcgacgggc tttcagtagt tgcaggtcgc ccagcagcgt
     5461 ggcatacaaa tcctgcatat gctcagcaac ggttgcatcg ctcattgaca gtacctgctt
     5521 tagctgcatc agcagcgtct gagccagtac tggctcgtcc gcgcagcgca gagtttgagc
     5581 atcaatccag agccagcccc agataccacg ttcagcgatg aagcgccatt gtgctcccgg
     5641 caggttaatg cagtagcggt catcgccctg agattccgcg tggaaaacct gctcatactc
     5701 cagctcagac aacatttttg ccaccaggcg gcggttgacc aaatcccagt ctttgtgatt
     5761 cattacagac cgacctccgt gaagaagtga tgacgatccg ccatcaccat gcgcgagcgt
     5821 ttatgtggga agtcgaactc tttaattgtc cggtatcccg caggctcaag atggcggaac
     5881 aggcgctggt tatcgatgcg tggctccagt actgtgcgct gcgtacgtgg ctcattcagc
     5941 agcaggtaat gtgtcacccc gcgcagccag ctttgcacat agtgggctcc gcgccattgc
     6001 tgttcaccaa ccagcagatg caggccacgg tcaaagggct gccacgaata gtggcgacca
     6061 atgcggtctt ccgccgccca gtagatttcg aaataactga acggccgatc gtcgaaacaa
     6121 ccaatcagcg ggaatgcatg tttactggtg agctggcgtt ccagataggc gatctgtacc
     6181 tccagtgagc cactttgctc ccagaaatac tcaacgcgcg gatcgttcat ccagcgagtg
     6241 aaacgttctg catcagaaac gggatcggca atgcggaaac tcagcatcct gcgaatgcgt
     6301 ggatcgtaac gacgataaac ttcaccctgc ggacgggggg ggcgctgcgg gaaataaatc
     6361 tcacgctctg catcaaaaac catttcaccg gaggcccggt ttgccggaga actcagccat
     6421 aacggtaact gccagaaagc ggaacgatgg ataatgtcgc tttgtacctg tccgaaaagc
     6481 gtcagcgcct gtggctcctc acaccattcg gaccagggaa gaaccactgc tgaaagttgt
     6541 ggtgcggcga taaatatctg atccagcgcg tcgcacagcc accctgttgg cagctccccg
     6601 ggccagtgca acaccgcgct attgtccagc ccccagccaa gattcagagg cttgtcgagt
     6661 ttttcacagc gcagtccata tccgctgtga acaatgtttg ccccagacat tcagacctcc
     6721 tgagcctgca gcgggttagc gaaatcaaaa tagatcaccg aagggtcaac gatggtgttc
     6781 tcgttatgat cgttcagata acagaaaaag ttacctttta cgttccagta cggactttcc
     6841 agaacatagt tgagacaggt tttatgagtc acctggtcac gcacttcagc cagcaatgtt
     6901 cgtacacgag ccatcagatt cgcttcgctg tccagcccgg cagcacccag cgcagcagtt
     6961 acggcaaaag tggagttaac cagcaggtaa taagggaaat agcgcagcaa ctgctcacgg
     7021 gtgaaaatat tttccgcctg cgcctcgtca atggtatcga gccactctgt cgcgtgaggc
     7081 ataaacgcgc tgccctggca gtcacggtaa ataaatccga ccggcagatc cccaagcatc
     7141 tgcacaagaa tattttgctg atgcgccagc agcaccaggc cgtaatccgc ttcagccgta
     7201 aacagaggct tcagcacctg ctgacagtaa gcatcgaccc atgcgtgggc ggcctgttgc
     7261 acagtgatac cgaggcgatc gctcaggcgt tttaccgccg aaaccagcag cgaatcaccg
     7321 ccatccggtg cggcctgagt cagggaaacc agaacgttgg tctggctttg cggctgctcc
     7381 agcagcaggt tttcacgcag ggaaaacaaa ctttcctgca tgatgttgcc gttaagatcg
     7441 cgcagcccgg cccagccgtc ctcctgcatt acccggaaag tagggaagcg ggcctgtagc
     7501 atctgccagc cgtcggtttg cgccagacgt gccaggcgca ttcctcgctc cacctctttc
     7561 acagacagag tacggacgga gttggtcagc cgaacgctca gggagaactt gatcatatcg
     7621 cggctggtag cacagtagag ggagcgggaa gaggtggtcg gcagccacga cgtgccggcc
     7681 tcgcctaagt ctctgataag tcctttagca aaaagtgcct ggcaccacac ttgctgcaaa
     7741 agatattctc cctgccacgg gtgcagcggg aacagccatt gattgtcact taattcgttg
     7801 agtaactggg gcgcattttc tgcggcaaat cgcgtcaacc gctgttgaag gttaagatgc
     7861 agactttcac cagcgatttg cgttttatcc accgaaaacc accgcagcgg gaagtgaggt
     7921 gccatgtcag gcaggtatcg ttcagcttcc tggcggttaa acggttcatg agacttaggt
     7981 gcagggtgga aagcgtgtcc tgtcagcagt gcctgctcag cctcgccaaa attcaacgct
     8041 ttttcacgca ggatggccca gtcatggcgg gcatcaattg tctgttgcgt atgggcgtag
     8101 ctttccagca cgcgttggtg gaaagtttcg cagaggggaa ctggcagaaa aagttgatgc
     8161 cggagcttat caataataag ccgggagaga gtggtaaagt cgacaggata actgcctgat
     8221 gccgtgacca gatgagcagg aaatgcaaag cggtgatgct gggttgggga gaaataagcc
     8281 actctgaaat ggagcgactt ctgttcgtcc agcgggataa tcaattcgtc tggcgggtat
     8341 tccgccagtt gccagtcttt ggtttcacga atcaaggcat tcaggaagca ctgcgcagcc
     8401 acatctgtgg cggatttttc agagggcagg atcataatca aacagctcct acacagtaaa
     8461 ataataacaa ttatgtcaat aatgattctc attatcaatg agaaatgtta ttgtcagaaa
     8521 aattaatttt acaaataaac acaattaata catggtgttg aaacattaca atgtcatcta
     8581 agatcgaaga tacgccccaa aaaaccctgt cctgctggcc actggcgttc agtgccggtc
     8641 ttctcggtat cggacagaac ggtctgctgg ttgtactccc tgttctggtc atacagacaa
     8701 atctgagtct gtctgtatgg gctgccctgc tgatgctggg ctcaatgctg tttctgccat
     8761 cttccccatg gtggggaaag caaatttccc ttactggcag taagactgtg gtgctgtggg
     8821 ctctgggagg atatggcgta agctttaccc tgcttgggct gggaagcgtg ctgatggcta
     8881 ccggtgccgt aacaacagcg gtggggttgg gaatattaat catcgcccgg atcgtctacg
     8941 gtctgaccgt gtcagcaatg gtgccagcct gtcaggtctg ggcattgcag agagcgggag
     9001 aagggaatcg catggccgct ctggcaacca tcagctccgg cctgagctgc ggcaggctat
     9061 tcgggccgct gtgcgcggcg gcaatgttgg tcattcaccc tctggcgcca gtgtggatgc
     9121 tgatggcagc tccggtgtgg gctgtggtga tgcttctgcg gttgcccggc acaccaccac
     9181 agcccacacc ggagcgcaag agcgtcagcc tgaagcggga ttgcctgcct tatctgcttt
     9241 gcgcaatgtt actggctgcg gcaatgagca tgatgcagct tggactttcg ccagccctta
     9301 ctcgccagtt cgatactgat accaccacta ttagccaaca ggtggcgtgg ttgttggggc
     9361 tgtccgcaat agctgcgctt atcgcgcagt tcgtggtact ccgtccacag cgcctgactc
     9421 cagtggctct gctcctgagt gccggggtgt tgatgagtag tggtctggct atcatgctca
     9481 ctgaacagct atggttgttt tacctgggct gtgcagtgct gtcatttggg gccgctctgg
     9541 caacacccgc ttatcaactt ttactgaatg ataagctggc cgacggcgca ggcgcgggct
     9601 ggcttgcggc cagtcacaca cttggctatg ggctgtgcgc attgttggtg ccactggcgt
     9661 cgaaaacagg tgtctcaatc gcgctgattg tgacggcatt atttgccgct gtattattta
     9721 ccatagtgtc tgcatgtatt tggcattacc gtacaatcaa gtaaggctat gatgttgtac
     9781 tcacagccgg cataacgtcc tgtgcaattt cgctgtcgaa accgcctgtg ctgaccgccc
     9841 agtgcactca cctgaagcac atcgcagcat gccaccagga tgccagatcg tcttctgaaa
     9901 tgggtggata cattggcaac gttaaccgca acaatttttc agagagaatt ttggggggtg
     9961 agggccagtg atgtaaaaaa tacgccaaga ggttaattca ttgtttatac tgatatgtca
    10021 ggtaatgcgt ttgcagtcat tacagtcgtc atggagactg taatgactgc aaacgcatac
    10081 catggagcga aattctatcc taagcgtcac attagttgat aaacctgtaa cgttaatgaa
    10141 ttctcatgcg aaaagtatgg ttatgataaa aaagcctaaa gctgtcatga tgagggagcc
    10201 aatgacgtga acaagtacag acgtcaaagc ccatatataa tttcccgcct ggagtaatgc
    10261 gaatacttca acagaaaatg tagagaaagt actcagacct ccacaaagtc ccgttgttat
    10321 cattaatttc caaaaaggat caaggtgtgg ttgtcgtaaa aagtacgcca acgcagtacc
    10381 aataattaac ccggcaagga ggttcaccac cagtgtacct ggaggtagat ttggaaaaag
    10441 actgttaaag cgtgtggaca gtaaccagcg cagagtacat ccaacagaac cgccgataat
    10501 gacggcaaaa agtgatttaa tcatgtaccc ctcataaaat aattttttat cgtaacaata
    10561 tcagaatatg ttctcatttg actgatattg ttaatttata tcctttgact acatgcaaga
    10621 taagggaagg ttctatttat tgacattaat accctcgaca attgctgagc agaagccccc
    10681 acgacaagtg acagaatgac aacatctaca gtggggagcg ttttatcgat gaacaagcaa
    10741 ccaagaagta acacggcata ggtgacgaca atgccagtaa gcatagaaat aacaaaaacg
    10801 tgagctttca tcattcctcc agaataaccc cgacgtgtaa gcctggggga ttggcacaat
    10861 aatgtcattt aaaggcagac tttccggcaa attgcgccga tgaacccagt tcatcctcaa
    10921 ttctcataag ctggttatat ttttcaatac gctcaccccg gcatggagct cccgttttga
    10981 gatgacctgc gcgcattgca actgtcatat cggcgataaa gctgtccact gtctcaccac
    11041 tgcggtgtga gataaaggtg ccccagttgt tgtcatggca aagttgtacc gcatcaaagg
    11101 tttcactcag ggaaccaatc tggttgagtt taatcaatgc tgaattggca aggttttcgt
    11161 caatgccgcg ctgaatatac tttacgttgg tcacaaacag atcgtcgcca accaattcta
    11221 tctgctctcc caatgcacca tgcaggattt tccatccagc ccagtcatct tcagctaccg
    11281 ttaagggcag gaagatcaac taggtgctct ggggctggtg accaatgcgg ttgtgctgtg
    11341 aaacacactt tacatgcagg aggcgttgtc acatcttcgc aaatcgggag aaactcctga
    11401 ggagtagcat ccggcaagat tatcgctgct gatacatagc catataaata tgctggggca
    11461 ttatacgtct tcgttaccgg aagatattct taagggtgag ttaagatcat taaacttcaa
    11521 tacaaacaat taattaaact cttagcgtgg ttttttacat cactggccct cgccccccaa
    11581 ttttctctat gttttgccac tacttccctc attttactgc gctgtttgct acccctgaga
    11641 atcgacatta cttgctatcc agtccgcagc aactctctgg caacaaaact tatcagaatc
    11701 ataagtctgt gcattattcg ttcaagcgca tcgccaataa gcggatgttg ctggccgagt
    11761 gttctgctaa tcggatgata acaaatcccg tggttcggca ttttcctttg tgcgtgcgtt
    11821 gatgctggta acggtaaacg tcatgatgaa catgaccgcg aataagacaa caattgtcgg
    11881 agctggcgcg ctgtcgataa aaaaggacag ccagacacca gtcattgaga ccaatatcga
    11941 tatgccagtc gccagcagga gcgcaatatg aaagcgttgt gtcaggagta ccgcaatggc
    12001 accaggcgca atgagaagag aaatagagag aatgatacct accgctttca gcgtcgccac
    12061 aatggtcagg gagaccatgc acagcagccc ataatgcagc catctcgtat gcaaaccgct
    12121 tgcctgcgcc tgttgataat caaagctgaa cagcaaaaaa tcccgccatt ttacgctaat
    12181 aacaagtgta accagtccgg caataatcac cgtctggatt atatcgccga tggttatccc
    12241 gagcatgtcg ccgaaaagaa tatgatcaag atgtacgtct ggtttgactg caatatacaa
    12301 gatcagacct gcggcaaaca tgccagaaaa aacaatcccc atcactgtat cttgcttgat
    12361 tcggctgttg tctttcaggt atcccgtcgc caccgcacag aatactccgg cgacgaatgc
    12421 ccctgtagcc agaggtaacc ccagaatcca ggcaagcacg atgccaggaa aaaccgcatg
    12481 actcatcgcg tcacccatca gtgcccaact tttcaggacc agaaagaccg acagcagcgc
    12541 acagggtatg gaaactacca gtgaaatcag cagtgcgtga ctcataaagg tgaactgcag
    12601 tggctccagt aataatgcca tcatgagcga tcctccagcg ctttatgcgc acgccgacga
    12661 tttgccaaaa gcccatgggt gggcgcaaag acaaatgcta aaagaaacag cagtgtctgc
    12721 gccaccacga tgattccacc tgtcgcgccg tccagaaagt aactgaccca cgcgccgagg
    12781 aaactggtca cactgccaat agttacagca atcatgagca ggcggggaaa acgatcggtt
    12841 aacaaccatg ctgttgcacc gggagtcacc accagacaga tcaccagaaa ggcaccaacc
    12901 gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga agaagaggat ttttaatctg
    12961 cccggatgca atccaatagc acgggcgtga ttctcgtcga aaaaggtcac cataagatct
    13021 ttccatttga aaaaaagcac gatgatcgag agaatgccaa tgatagtgag ttgcaggata
    13081 tccgccggat cgattgccag aatattgccc aggacgatgg tctgaatgtt cacggatgtc
    13141 gggttcaatg acaccataaa cagccccagg ccaaaaaagg acgagaagat caggccgatg
    13201 atggcatctt cttttaagcg agtgagttgg ttaagaaaca gcatgctgcc cgctgccagt
    13261 ccgccggaaa agaaggctcc cagagaaaac ggaagcccca gcatatatgc ccccgcaaca
    13321 cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg accagccttt tagcatcaga
    13381 tagcatgaca ggaaagcgca gagaccgccg accatcgccg agacccacat cgcattgagc
    13441 atatattcat agctgaaggg ttccagtagc gcgttcatct ttcttccctc tgcaccgctg
    13501 acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat aatgctttct tccgagccgt
    13561 taagtgtgac atggcgcagt acgccgctaa aagccagctc caggttctcg gctgtaaaag
    13621 tggtgtctgt cggcccgctt gccaggacgg tgcctttgac catgaccgta taatcgcaaa
    13681 atgtcgtgac ggatccgaga ttgtgcgttg agacaagcat cgttttgcct tccgcgcgta
    13741 actccctcaa caggctgatg attttcgctt cggttttcac atcaacgccg gtaaaaggtt
    13801 catccagcag gatcacatca ccctgctgtg caatcgctct cgccagaaag acgcgtttct
    13861 tttgtccacc ggaaagctcg ccgatttgtc gatggcgaaa atccaccata tcgacacgtt
    13921 ccagcgcatc ggtgacaatc tgacgatccc tctttttggc gatacgcaac atacccatat
    13981 gcccatagcg gcccatcatc accacatctt ccaccagtac aggaaatgac cagtcaacct
    14041 cttctgactg agggacgtag gcaaccaggt ttttctgtag cgcctgtcgt gtgggaatac
    14101 ccagaacaga tatcttcccg ctggtcagac gcacaaatcc cataatcgct ttaaacaacg
    14161 tcgattttcc ggaaccgttt accccaacca gagcggcaat agatccgcct ggtactgtga
    14221 aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt gacagtaaca tcattaacga
    14281 caatgcctgc agattgcatc attatttctc cctttttccg gctttaattc cctgtaccag
    14341 cgtactggta gtaaccttca gaaggtcgat atacgtaggt accgggccgt tttctgtgct
    14401 cagggaatcg acatagagca caccaccgta gtgcgcgccg gtttcacgcg caacctgacg
    14461 cgctggttta tcggaaatcg tactctcgct aaagactgcc gggatatgat ttttcttaac
    14521 tatatcaaca accttacgta cctgctgcgg tgttccttgt tgatcggcat taatcggcca
    14581 cagataaagc tcttttagcc ccaaatcgcg tgcgagataa gaaaaagccc cttcactggt
    14641 gaccatccat cgctgattct cagggagttc cgcaatctgc ttacgcaggg gggcaagggt
    14701 ttgggtaatc ttggctttat aagtatcggc attacgttgg taggtttgtg aatttgccgg
    14761 atcgtatttt ataaacgcat cacgaatatt atcgacgtaa atcagagcat tatctggcga
    14821 catccaggca tgggggttag gtttgccctc atagggccct tcggtgatcc ctactggcgt
    14881 cacacccgaa gagacaatta cttctggaac cccattgaga tgctggtaaa agcgttggaa
    14941 ccacaattcc agattcatac cattggcgag aatcagttgt gccccctgcg cacgtttaat
    15001 atcgccaggg gtaggctgat actcatgaat ttctgcacca ggcttggtta tggatgagac
    15061 ttctgcagca tctccagcca cgtttttggc catatctgcg atgatggtaa aagttgtaat
    15121 gaccttgaat ttttcagctg cactcgcctg aaatgctatc gagcaggtga ggactaggca
    15181 ccccaagagc atggttactt tttttattga gagcatgatt tagtacctat agtgagcaaa
    15241 attagaataa acatagcaac tactatgttt tatagcagaa gctataaaat gtaaataatt
    15301 atcattagcg attataagat cccaacacag ggattaggtt atttcctgct gacgatgtgg
    15361 tattgaggat aaaagttggg cagcgctttg gctttaaaat gaacttgttc aggacgattg
    15421 gtaatggcaa agcaaaaata atgtaggtta ttggcaagtt ctctaggacg caggagagca
    15481 tgaaaaaccc aggggcagtc actaaatgaa ggga
//
LOCUS       DAZFZC010000105        15137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFZC010000105 DAZFZC010000000
VERSION     DAZFZC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.106"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..408
                     /locus_tag="ACHKXP_004000"
     CDS             169..408
                     /locus_tag="ACHKXP_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7648910.1"
                     /translation="MSLNFKWLNWLYNQGFLLPAREHDINDNLLFKWLRLWLIKGRIS
                     RRLPVRNSSGIGVELLPVEMTLDERPYPAFYARLL"
     gene            complement(619..1875)
                     /locus_tag="ACHKXP_004001"
     CDS             complement(619..1875)
                     /locus_tag="ACHKXP_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7648911.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINILIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSAEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADVVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFML
                     YLLNMTVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(1888..2175)
                     /locus_tag="ACHKXP_004002"
     CDS             complement(1888..2175)
                     /locus_tag="ACHKXP_004002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7648912.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            complement(2191..2634)
                     /locus_tag="ACHKXP_004003"
     CDS             complement(2191..2634)
                     /locus_tag="ACHKXP_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7648913.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILMAKTTQDILSVIARY"
     gene            2905..3936
                     /locus_tag="ACHKXP_004004"
     CDS             2905..3936
                     /locus_tag="ACHKXP_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7648914.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NSDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIATGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            4064..4387
                     /locus_tag="ACHKXP_004005"
                     /pseudo
     CDS             4064..4387
                     /locus_tag="ACHKXP_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(4463..5383)
                     /locus_tag="ACHKXP_004006"
     CDS             complement(4463..5383)
                     /locus_tag="ACHKXP_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFO7648915.1"
                     /translation="MEGGATGTALHTGWQTVGGGYGDMDLEKFFDLVDHRPLMTRLAR
                     TIKDRRVLRLIRRYLKAEMVKDGQREKRQAGMPQGGPLSLLLSNILLDELDKELERRG
                     HSFCRYADDCNIYVSSRKAGDHLLKNIRAFVENKLKLKVNEKKSAVARPWERKFLGYS
                     VTWHKQAKLKIALTSVNRLKEKVRSLTTGNRSKSVKVTINALTPVLRGWISYFRLTEV
                     RGVLEELDGWIKRKLRCLLWRQWKRPGTRTKNLLRVGLSKDRAMISAYNNHGPWWNSG
                     SRHMNQAIKNAWFSRQGLISLLEQQRQFQC"
     gene            complement(5287..5721)
                     /locus_tag="ACHKXP_004007"
     CDS             complement(5287..5721)
                     /locus_tag="ACHKXP_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFO7648916.1"
                     /translation="MEAVITRENLMLAYQRVVENKGAAGVDNLSVAELKPWLKKNWRS
                     VRQALIDGNYQPRAIRRMDIPKPDSGVRTSGIPTVVDRLIQQAIAQQLSAIVDKSFSD
                     SSYGFRPRRNAWKAVQQAQRYIRGGKRWVVDMEIWIWRSSST"
     gene            6630..6950
                     /locus_tag="ACHKXP_004008"
                     /pseudo
     CDS             6630..6950
                     /locus_tag="ACHKXP_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(7166..7510)
                     /locus_tag="ACHKXP_004009"
     CDS             complement(7166..7510)
                     /locus_tag="ACHKXP_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648917.1"
                     /translation="MVDRMYVPPLASRRVKQTQIRSRDKWAETDLFVEQGVRYFFRVI
                     GKWCDMNHCCDANGYTVDYLNFARIWLRCRHEPATWFALIGTIDKSTDTMFVIGDGAR
                     LNNGWLRLAAVS"
     gene            complement(7865..9214)
                     /locus_tag="ACHKXP_004010"
     CDS             complement(7865..9214)
                     /locus_tag="ACHKXP_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7648918.1"
                     /translation="MMNNNIGKPKMTHYRWWVMSFIFVTYTVANADRANIGFAIPFIQ
                     EEFQISNTMAGLLISLFFAGYAFFQIPAGYLIKKFGMRNAFAMGMLLTSVFTGLMGMV
                     NNIFLLKILRFMVGICESPVVIGSTVTINQWFPPKEKGTATGLFLAGSKFGPLIVPPL
                     CAWIITVWGWRYIFVFFAIPGLLLAIFWYLMVRNKPEESRFVNEQEVAYIREEQLPTT
                     TQEAAAPVAPEFNYCWLDKLIRTKQVEELSCSKQVFRCWDIYGVALGYFSMVGIVSVL
                     MSWLPKYLITERGFDLTSSAALAAAPFFGTVMGNFIGGVLSDRVLNKRRKPLMLLSAG
                     ATIFTMYALVYAPESKIVLSLMLFLLGLMLSLGYSAYSVYAMGRANKDIYPIAYSVIN
                     MGGQLGGMCMPLIVGIILDAYNWDAVFMAMTGFCVLCFILVSTVIEPLSKNMKVNYR"
     gene            complement(9235..10152)
                     /locus_tag="ACHKXP_004011"
     CDS             complement(9235..10152)
                     /locus_tag="ACHKXP_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HFO7648919.1"
                     /translation="MSTFIQITEVGPRDGFQNIKAFIPTDLKIKIIDRLVESGIGAME
                     ITSMVSPKAIPQMRDAREVIQHVLNTHPHIIPSVLVPNVKGASLASEAGIQNINYVVS
                     VSPAHNKANINRTHAESLEDLYKIRHTFPEMTITLSLATVFGCPFIGETSRETLMDMI
                     KFADDLGISAVTLCDTIGVANPTQTLSVLRGIQQKFPKMDIGLHLHNTHGMALACMFA
                     GVQLGITRFETAVGGLGGCPFAPGAAGNAATEDAVNMFNRMGFDTGISLPDLLDIANL
                     IRSTIEPTLLSSLSRARTYSEFDFCSEKI"
     gene            complement(10164..11360)
                     /locus_tag="ACHKXP_004012"
     CDS             complement(10164..11360)
                     /locus_tag="ACHKXP_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HFO7648920.1"
                     /translation="MNKQSGVLSGVRVLDLTRVLAGPYCGMMLADFGADVIKIELPGK
                     GDDSRANAPQINGESAYFMNLNRNKRGMTLNLKSEEGRQIFLDLVRDSDVVLENYRPG
                     VMEKLGLGYEDLRKVNPAIIYGAVSGFGHTGPYSKRAGYDIIGQAMSGLMSTTGWPDT
                     PPTRTGTAIADVVGGMSCAIGVLAAYVNRLKTGVGEKVDIALVDSMVSSLEIINIIYL
                     NTGRIPTRIGNRYEAIYPYDSFQARDGYVIIACGNDKLYGLLKNVLQISALEDEKFKS
                     NLDRVAHHAELKEIIECWTRDLDIDTIVSMLLDAGIPSAPINTIDRVTQDPHIAGARE
                     MFVDIEHPVAGKITMTGNQVKFTHNKATIRMPAPTLGQHNYEVLKERLNYSDEKIEKL
                     IRSGIL"
     gene            11597..13510
                     /locus_tag="ACHKXP_004013"
     CDS             11597..13510
                     /locus_tag="ACHKXP_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO7648921.1"
                     /translation="MSHKTLPNLALITPYPALAAVVTEQCTGIFNCHVFHGSLEKAAG
                     IARNLDPDFYDIIISRGGTADYIDRVTKIPVVRILTSALDLLKSLTPLKKHPHKIAFF
                     NYQNYLSDVNLVATALEVEIEEFIFLSHADMVDKLKKCRTRNFQTVAGGLPVSDTAEH
                     YEMQGILIENGLESVNSALREAMAIVETTRRKQQDMARLEIILSSITEGIIVTNENNE
                     IQIFNKSAENIFGLMASQVIGKPVDTVIKNTRINQVLLTKSAEIREIMDLDNTSIITS
                     RVPIFRGTQCIGVVCSFTDTPQIQKVERIIRGKLMKKGFTARYRFEHILTQNKHMESV
                     IHLAKIYANTHSPVMIYGESGTGKELFAQSIHNQSQCHRGPFVAINCAAIPETLLESE
                     LFGYEGGSFTGARREGKEGLIELAHQGTLFLDEIGELPLPIQSRLLRVLQEYEIMRVG
                     GKDIIPVDVRIIGATNRSLEKMAEEGSFRGDLYYRLNVLPLMVPPLREREGDVRFLAE
                     HFLEKSGCGHKLAQFIALFSTYNWPGNVRELNFIIERLALLSAGFPNASLFEILNLTG
                     FNALKSCNTSQTNGRIVADLSKGQLKAVIKDLERQIIAFYMDLFEQDQEKVAAHLGIS
                     KMSLWRKYNQMDR"
     gene            13761..14628
                     /locus_tag="ACHKXP_004014"
                     /pseudo
     CDS             13761..14628
                     /locus_tag="ACHKXP_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061069586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
     gene            complement(14662..>15137)
                     /locus_tag="ACHKXP_004015"
     CDS             complement(14662..>15137)
                     /locus_tag="ACHKXP_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7648922.1"
                     /translation="TGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC"
BASE COUNT         3881 a         3992 c         3548 g         3716 t
ORIGIN      
        1 cgttatattc atgaggccgg agtgcactat aaaagtgtat gcgtaaaccg actgctgtat
       61 gtagccatta agccagtatt ggtaacatag gtacccacct tttcaactag tggacacctg
      121 ttatggaaca aaaaacatta tcagctgaac cccgcaggtc atgttcaaat gagtttaaac
      181 ttcaaatggt taaactggct ttacaaccaa gggtttctgt tgcctgcccg ggagcatgat
      241 atcaatgata atcttctgtt caaatggctc aggctctggc tgatcaaagg gcgtatatca
      301 cggcgtcttc cggtaaggaa ctcttctggc attggcgttg aattattacc tgtcgagatg
      361 acgctggatg agcggcctta ccccgctttt tacgcgaggt tattatgaag atcaatgact
      421 agccgtcgaa ccgtgtgcat gaactgctgc actgaaactt ctcaactgta aaataatcct
      481 tacactacgt acttctcgga gcgcttacga ttaatcagat gcagtcctaa ctggaagaaa
      541 gaaaaatgga aaaaaataag aggaattaat atcctccacg ttcctgaaat cctggaggat
      601 attaatgtca ttaaaatatt agcgaacgat aatccctaac aaaataccca gtgcgccaaa
      661 gtcagcatca ctgaaggtgg tgttagcaaa accaatatca cccagcacag gcaataagaa
      721 tactggcagg aaggtaataa gcaagccctg agcgaaggcc cccaaaatcg caccacgacg
      781 tccacccgtt gcattaccga atacccctgc agccgcacct acgaagaagt gcggcaccac
      841 accaggaata atcacggtca tgttcagcag atacaacatg aacataccga ttaaacctgc
      901 agcaaagctg ctcaggaaac caaccagtac agcgttaggt gcgtaaggga agacgaccgg
      961 gcaatcgaga gcaggtctgg cattcggaac cagcttgtct gaaatacctt tgaatgccgg
     1021 aacaatctcc gcaatcacca tacgcacacc ctgcagaatg atgtacacgc cagctgcgaa
     1081 ggtaatggat tgcatgatgg agaacatgaa ccagttttta ccaccgctaa gctctttcac
     1141 cacatctgcc ccggcaaaca ggcacgtcac caggaaaatt atgctcatgg taaaagagat
     1201 agcaaccggc gtgtcgcgca agaacagcag acttttcggc acgttcatgt cttcagccga
     1261 gtgctcttta ttaccaaact tgctgccgat gaaaccggcc aatacataag ataatgttga
     1321 gaagtgaccg atggcaacgt catcagaacc ggtcactttc ttcatgtacg gatgcgctat
     1381 agccgggaaa aagaccattg ccacgccaac aaccaaagag ccaacggcta taagcgtaat
     1441 gccagtcatg cctgctgttg ccagaattac cgctaccatc atcgacataa acaacgtgtg
     1501 atgaccggtc aggaagataa atttccacgg tgtgaaacgg gcaatcagaa tgttgataac
     1561 catcgcaaag aacataatca ttgccatttc tttcccgaag cttttctgcg ctacggaaac
     1621 aatggcttca ttgttcggca ctacaccctg aataccaaag gcatgctgga aaatgtttgc
     1681 gaagtcaccc agtgacgaca caactaaacc cgcccctgcg cctaaaatca caaaccccat
     1741 aatggtcttg acggtacctt taatgcattc ggttaccggt tttttctgtg caatcaggcc
     1801 aatcagggcg attaaaccga ccagcactgc gggttcggaa agtacatcgc tcatcaaaaa
     1861 acgaaagaaa tccatatcgc ccccagctta cagtgcgcct aattcggtta ataccacgga
     1921 taaacgttct ctcatggctt tcttatcaat catattttcc agtgcgacga ttttgcctgc
     1981 gacagactgc gcgactaact gctcggcaat gtctttggtg ccgacataga tatcactcgg
     2041 cgtccctttc gcggaaccca ggtcgacgtg atcaacatca gcgctgacgg acaaatcctt
     2101 caaaatgctt ttaatgctca tttccatcat caggctggtg cctaagccgt taccgcaaac
     2161 cactgtaatt ttcataaatg ttcctctgga ttaatagcgc gcaataacag acaaaatgtc
     2221 ctgcgtcgtt ttggccatca aaatggcctg tatgtctttt tcgttatcga ataacttggc
     2281 gagttgagaa atcgcttcaa tgtggctggt actgtcggtt gcagccagca caatcagtaa
     2341 tttcactgga tcgttttcat cggcatcaaa attcacacct gaagggatca gggtcagtgc
     2401 taatgacagc ttgttagctc cctcttccgg gcgcgcatga ggcatcgcaa taccagggcc
     2461 aaccacatag tacggaccga tagtgtcgtg cgagcgatag atcgcatcaa cataacgcgg
     2521 ttcgatagaa ccgttttcaa tcaacggccg gcatgaaatc gccacggctt cccgccagtc
     2581 tttggcctgt tccacgacct gaacgacctc tgtatttaat agatttttta acatgctact
     2641 gataactccc gatgataatt aacaggagag tagatatttt acgcattaat taatgtgatg
     2701 attgtcgcat ttgatagcgc tatctgataa cgctaccaat ctttgtgata gcgctatcat
     2761 ttctctgccg ccatgctttg gctatattcg gttcctgcat atacaatgca acaggatatt
     2821 tgctaccgtg ggcaaaaact ggaaagagta atggtgaaat aaaagtaatg aaaacagctg
     2881 gttgtaatta tccatggagt cgaaatggaa aaaacacgta agcgccgtgg taccggacgg
     2941 gtgacgctgc aggaggtcgc taactttgca ggtgtcggaa ccatgaccgt ttcccgtgcg
     3001 ctgagaacac cggaacaggt atctgacaag ctgagggaaa agatagaaca ggccgtagaa
     3061 gagttgggtt atattcccaa cagaactgct ggcgcccttg catctggtca cagtgataca
     3121 gtcgccgtac tggttccttc cctgacagat aaagccagct cccgctttat gcagtcgttg
     3181 cagcaggtgc ttaataaaaa tgaattccag ctactgcttg ggtgtcatga gtataatcag
     3241 ctcaaagaag ctgaaattct gatgacgctt cttcaaggca acccggcggc gttagttatt
     3301 tttggttctc agttggctga taaaactcac cagatactcg agaaaacaaa tatacctacc
     3361 attaacgttg tcggttcccc tttcagtccg gctcaaatta ccatcgaaac agcatttttt
     3421 gaagcatcac ataaactgac cgaacatctg ctggaacagg gttataagaa tatcggattt
     3481 atcggtgcgc atatggacaa ccgcctgcaa cgtcagcaac tgaacggctg gcataaagca
     3541 atgttgagcc attaccagaa ctctgatctt gttatcacca tgccggatgc cgcaagtctg
     3601 caattggggc gttacgcatt aaacgagata ttactgcgac aaccagagct ggatgctgtg
     3661 atttgcagcc acgaagagat tgcgctgggt atcatgtttg agtgccagcg gcgtttgtta
     3721 aaaattcctg gaaatatcgc tgtagcttgt cttgatggtt cagacaattg cgatcaaaca
     3781 cacccaacct tgacatccat ccgtattgac tacaaaaaaa tggggatagc gacgggtaag
     3841 ttactgattg ggctattgaa taataatcat gatgaatcag aagagtcgcg tatcgtgcaa
     3901 ttcaactacc agatagaatt acgtcagagt acatgagcct aaaagggcac tattacaaag
     3961 ttagctattg tggtagggat agccgtttcc ggctattccc cgcgcagatc cggatgtatg
     4021 ctgctaacgc atccattcct acttcaggta tttggcgtag aagcggatgc caaaaacttg
     4081 ccgcaaaacc gcaatatcag cctgcagagt cgctctccag attatccatt cctatattat
     4141 ctctgcaccg gatgaattac gcgaaccact cagaaatatg acacgtatgc aacttatcag
     4201 gactccggga tcctggaagc ctagtgccag tgaatatcgt aatgttacca acgtttatcg
     4261 cattgcattg aaatcacttg cctgacgcta tttcgagtta catgatgaaa tcgccgacct
     4321 taatgtcatg attgcggcaa ttgtcgatga actggcacct gaactgatta aacgtaatgc
     4381 tgttggatcg agtaggaggc gggatcactc ccgccgtcct ctcacaccac cgtacatgcg
     4441 gctccgcata cggcggttca tgttaacact ggaactgcct ctgctgttcc agcagtgata
     4501 tcagaccctg cctgctgaac cacgcattct ttatcgcctg attcatatgg cgtgaccctg
     4561 agttccacca tggcccgtgg ttgttgtacg ctgagatcat cgccctgtcc ttactcagcc
     4621 ccactcttag caggttcttc gtccgagttc ccggcctctt ccactgccgc cacaacaggc
     4681 accgcagttt ccgtttgatc cagccgtcca gctcttccag cactcctctg acttccgtca
     4741 gcctgaagta gcttatccat ccacgcagca ctggcgtcag ggcattgatt gtcactttca
     4801 ctgatttgct ccggtttccc gtggtcagac tgcggacttt ctccttcagc ctgttcacac
     4861 tcgtcagggc tattttcagc ttcgcctgtt tgtgccatgt cacactgtac ccgaggaact
     4921 tccgctccca cgggcgcgcc accgcgctct tcttctcatt caccttcagt ttcagtttgt
     4981 tctccacgaa cgccctgatg ttcttcagca gatggtcgcc cgctttccgg ctgcttacgt
     5041 agatgttgca gtcgtctgcg tagcggcaga agctgtgtcc acgacgctcc agttctttat
     5101 ccagttcatc cagcaggatg ttcgacagta acagcgacag cgggccgccc tgcggcatgc
     5161 ccgcctgccg tttctccctt tgcccgtctt taaccatttc cgctttcagg tagcggcgta
     5221 tcagtctcag cacccgcctg tcctttatcg ttctggccag acgcgtcatc aggggtctgt
     5281 gatccactag gtcgaagaac ttctccagat ccatatctcc atatccacca cccaccgttt
     5341 gccaccccgt atgtaacgct gtgcctgttg caccgccttc catgcgttgc gcctcggccg
     5401 gaagccgtag ctggagtctg agaagctttt gtccacgatg gcgcttaact gctgcgctat
     5461 cgcctgctgg ataagcctgt ccaccaccgt cgggatgcct gaggttctca cgccgctatc
     5521 cggcttcggg atatccatcc ggcgtattgc ccgcggctgg tagtttccgt caatcaatgc
     5581 ctgcctgaca ctgcgccagt tcttcttcag ccacggtttc aactccgcca cgctgaggtt
     5641 atccaccccc gccgcgcctt tgttttccac cacgcgctga taagccagca tcaggttttc
     5701 tctcgttatc accgcttcca tcgtcagcgg cacttccgct ttcgtttgcc cacccgccgc
     5761 cgtgctgatt tcagcgccat catgcagccc tgacggatac cgtccgtctc ctctgccgct
     5821 ggccgcagtt gcctgcgctt atgctttatt aatcatcatc gggttcggat gtcctaatca
     5881 cggttaatca tgttcggccc tgcatgctgc cagttttgca gcactactac ggcgtcggct
     5941 gacttctgcc gccccatccc ggcaccttac gacgccggta gcacactggc aggacggcag
     6001 atctcccggg gtaattcgcg cgactttcct gcttatgcct gtcggctcta cgtcacagcg
     6061 ttccgtgtga gtatcgggct ttggtgatgt atgccacctt acccgctgcg ccgccttaac
     6121 cgcttcctgt tcgtcaggcc agcattttgc atccggcttc cttcagattt cacctcgcga
     6181 tgaacaccct tgccttccgc taacacttcc cctcaccggg tgtgtggagg actttcacct
     6241 cttagtcgtt cttcagccac cacagttgaa gaacagcgcc agtcacggcg ctacgcgcca
     6301 tgcccggcgc acataccgga ggcggtgatg gcgggctcac caccgctgaa catcagcgtc
     6361 tgaaagagct ggaacgtgaa aatcgtgcat aatcgcatct gaatcgatca ccaattcgca
     6421 tcactatcga tcatcatttc gcatctcagt cgatcactga ttcgcatggt ggtcgatcac
     6481 tgtttcgcat cttccgatca gcgattcgca ccatgccgat cactccggga attaacactt
     6541 ttttgcatgg tttccactgc tgagtctcgt tgtctgtgga ggccataata gctaaaaaga
     6601 agaaatccag gagcactatg aggagcacta tggaaacatg ttttaaagtc ctgcagctca
     6661 aattcgaccg gaagctgtcc aacaggacta tcgggctgac actaaatatc agcccttcca
     6721 ccgtctttga ggtgctggct cgcttcaaag catcatcaac gccatggccc ctgccagaaa
     6781 cgctgacgcc ggaggccatt gaatcgctta tattcccgga gaaaaaagcg tctgtgctga
     6841 taccggtgct gcccgactgg cttcacattg ataccgaaat gcggcgaccg ggggtgacgc
     6901 ggcaacttct gtggacggaa tacagggctc aggacggaaa acgggcactg atataaatca
     6961 attttaaagg ccggaattag ccagcttttg gtaagaatcc gacgaagcgc acaaataccg
     7021 actgcttttg tacactaaat tttacgccat agagaatacg tgtaatcaga gagtaatatc
     7081 gacttcgctt ttcatcgcca gacttccagc actaccaaac caaagttatt ccagtacatc
     7141 cctgaaatat catttgcaaa gaccactaac tcaccgctgc gaggcgcaac cagccgttat
     7201 ttagccgggc accgtcgcca atcacaaaca tggtgtcggt tgatttatcg atagtgccaa
     7261 ttaaggcaaa ccaggtcgca ggttcgtggc ggcaacgcag ccagattcgg gcaaaattca
     7321 gatagtcgac ggtataacca ttcgcatcac aacaatgatt catatcacac cactttccga
     7381 tgacacgaaa gaaatacctg acgccctgct caacaaacaa atccgtttca gcccatttat
     7441 cgcgtgagcg aatttgggtt tgcttcaccc gccgtgaagc cagtggcgga acgtacattc
     7501 gatcaaccat acatcatcac ctttgtgcaa atacagatag gcaggccgta aaagaccgcc
     7561 cggataaaac gatgacgaac agaaggggga actctaattt ttatgaactc tgcggccatg
     7621 ctccataact ggaaagtcgt agtcttcgcg tgccgtttca tacctttcat tgacttggcg
     7681 cgatcgcacc tacagatgct cagtccattt actgaaccgt gactggggtt gcccttagcg
     7741 tttaagcacc aggagagcgc aattcaaatc agttatccag gccagatgac aacagtgatg
     7801 ggcggaacac ccggacggcg acgtgcggat aagcctcgcg cttatccgca catcatcacg
     7861 gcggttaccg gtagttgacc ttcatatttt tcgacaacgg ttcaatgacc gtggagacca
     7921 gaataaagca cagaacacag aacccggtca tcgccatgaa caccgcatcc cagttgtaag
     7981 catccagaat aattcccaca atcagtggca tacacattcc gcccaactga ccacccatgt
     8041 tgatcacgct gtaagcgatc gggtaaatgt ctttattcgc ccgccccatc gcgtagaccg
     8101 agtaagcgga ataacccagc gacagcatca gtcccagcag aaacagcatc agcgacagaa
     8161 cgattttact ttccggcgca taaaccagcg cgtacatggt gaagatcgtc gcccctgcac
     8221 tgagcaacat cagcggttta cgacgcttgt tcagcacccg gtcagaaagc acgccgccga
     8281 taaagttgcc cattaccgtc ccgaagaatg gcgccgccgc cagcgcggca gaactcgtca
     8341 gatcgaatcc gcgttcggtg atcagatact tgggtagcca ggacattagc acgctgacga
     8401 tccccaccat cgagaagtat cccagcgcga caccataaat atcccagcag cggaagacct
     8461 gttttgaaca ggacagttct tcaacctgct ttgtgcgaat cagcttgtcg agccagcagt
     8521 aattgaactc aggcgcgacg ggtgcggcgg cttcctgggt cgtcgtcgga agctgttctt
     8581 cgcggatgta agcgacctct tgctcattaa cgaagcggct ttcttccggc ttattgcgaa
     8641 ccatcagata ccagaagatc gccaacagca agccaggaat ggcaaagaag acaaaaatat
     8701 agcgccatcc ccagacggtg atgatccagg cgcacagcgg cgggacgatc aacggaccaa
     8761 acttggatcc cgccagaaac agcccggtcg ctgtcccttt ttccttcggg gggaaccact
     8821 ggttaatcgt caccgtcgag ccaatgacca caggtgactc acagatgccc accataaaac
     8881 gcaggatctt cagcaggaaa atgttgttca ccatccccat caaccccgta aacacggagg
     8941 ttaataacat acccatcgca aaggcattac gcatgccgaa cttcttaatc aagtacccgg
     9001 cagggatctg aaaaaacgca tatccggcaa agaacaggct gattaacaac ccggccatgg
     9061 tattcgagat ttgaaactct tcctgaataa aaggaatagc aaagccgata tttgcgcggt
     9121 cggcattagc cacggtgtag gttacaaaga taaaactcat gacccaccat ctgtaatggg
     9181 tcatcttcgg cttcccgata ttattattca tcatgtctgt ttccgtatca gaggtcagat
     9241 tttttcgctg caaaaatcaa attcactgta ggttctggca cgtgaaagac tgctcaacaa
     9301 cgtcggctca atcgtgctgc ggatcaggtt tgcgatatcc agcaggtcag gcagtgagat
     9361 accggtatcg aaacccattc gattaaacat attcaccgca tcctcggtgg cggcgttacc
     9421 ggcagcccct ggcgcaaacg ggcacccgcc cagtccaccg acagcggtct caaagcgcgt
     9481 gatgcccaac tgaacgccag caaacataca ggccagcgcc ataccgtggg tgttatgcag
     9541 atgcaaaccg atgtccattt tggggaattt ctgctggatc ccacgcagaa cggataacgt
     9601 ttgggtcggg ttggcgacgc caatggtatc gcacagggta accgccgaaa tcccgagatc
     9661 atcggcaaac ttgatcatat ccatcagcgt ttcacggctg gtctcgccaa taaacggaca
     9721 gccaaatacc gtcgccagcg acagcgtgat ggtcatctcc gggaacgtgt ggcgaatttt
     9781 ataaaggtcc tccaacgatt cggcatgcgt ccgattaatg ttggccttgt tatgcgccgg
     9841 gctgaccgaa accacatagt taatattttg aatgcctgcc tcactggcaa gactggcccc
     9901 tttcacattc ggtactaaca ctgatggaat aatatgcgga tgcgtgttca gaacatgctg
     9961 aataacctca cgagcatcac gcatttgcgg gatcgctttc ggactgacca tcgaggtgat
    10021 ttccatcgcg ccgatccccg actcgaccag acgatcgata atttttattt ttaaatcggt
    10081 aggaataaac gctttgatgt tctgaaaacc gtctcttggc ccgacttcag taatctgaat
    10141 aaatgtcgac atagtagaat cccttacaga atgccggaac gaatgagctt ctcaattttt
    10201 tcatctgaat agttcagacg ttctttaagc acttcgtaat tgtgttgacc aagcgtgggc
    10261 gcaggcattc tgatggtcgc cttgttatgt gtgaatttaa cctgattgcc ggtcatggta
    10321 attttccctg cgacggggtg ttcaatatcg acaaacattt cgcgggcgcc agcaatatgc
    10381 ggatcctgcg tcacgcggtc gatggtattg atcggcgcag aaggaatacc ggcatccagt
    10441 agcatcgaca cgatggtgtc aatatctaaa tcacgggtcc agcattcgat aatttctttc
    10501 agctcggcgt gatgggcgac gcggtcgagg ttagatttaa atttctcatc ttccagcgcg
    10561 ctaatttgca ggacgttttt cagcaggccg tataatttat cgttgccgca ggcaataatg
    10621 acgtaaccgt cacgcgcctg gaaagagtca tacggataaa ttgcttcata gcggttacca
    10681 atacgcgtcg gaatacgtcc ggtattaagg taaataatgt tgataatttc cagcgacgac
    10741 accatcgaat cgaccagcgc aatatccact ttttctccga cgccggtttt caggcggttg
    10801 acgtaagcgg caagcacgcc aattgcgcaa ctcattcccc cgaccacatc cgcaatagcg
    10861 gtcccggtgc gggtaggcgg cgtatcaggc caaccggtgg tgctcatcag tccgctcatg
    10921 gcctggccaa taatgtcgta acccgcgcgt ttcgaatacg gaccggtatg accaaagccg
    10981 gatacggcgc cgtaaataat tgccggattc accttacgta aatcctcata gccaagtccc
    11041 agtttttcca tcacacccgg acggtaattt tccagtacca cgtcgctgtc gcgaactaaa
    11101 tcaagaaata tctgccgacc ttcttcagat ttaagattca gcgtcattcc gcgcttattg
    11161 cggtttaaat tcataaagta ggcactttcg ccattgatct gcggcgcgtt ggctcgtgaa
    11221 tcatcccctt ttcccggcaa ttcgatttta attacatctg cgccgaaatc cgccagcatc
    11281 attccgcaat aagggccagc cagcacccgg gttaaatcca gcacacgaac gccacttaat
    11341 acaccagact gtttgttcat tctttccctc acttttttat tagatagagc acaaggccgc
    11401 tcgccttata gtttgtctaa tccaagatgt gtgccagaaa tgaataaaac agaaataaat
    11461 aacctaagta tatgatataa ataaccttac tacgttttac tatttatctg agagagagga
    11521 aaatggcttt caattttgaa atctataaca gttgtgatat tggtttgtga tagccacaaa
    11581 ttttccatgg tgatttatgt cacataaaac gttacccaat ctggccttaa ttacccccta
    11641 tccagcactg gcagccgtag tgactgagca gtgcaccggt atttttaact gccatgtttt
    11701 tcacggaagt ctggaaaaag ccgcggggat tgcccgtaat ctcgaccctg acttctatga
    11761 catcattatc agccgtggcg gtacggcgga ctatatcgat cgcgtgacca aaattccggt
    11821 agtacgtatt ctcacgtccg cgctggattt gcttaaatca ctcacgccac tgaaaaaaca
    11881 tccgcataaa atcgcctttt ttaattatca gaactattta tctgacgtaa atctggtggc
    11941 aaccgcgctg gaagtggaga ttgaagaatt tatctttctg tcgcacgccg acatggtcga
    12001 taaactcaaa aaatgccgta cacgcaattt ccagacggtt gcaggaggat taccggtgtc
    12061 agacacggcg gaacactatg agatgcaagg aattctgatc gaaaatggtc tggaatcggt
    12121 gaacagcgcg ctgcgcgaag cgatggctat cgttgagacg acccgccgca agcagcagga
    12181 tatggcgcga ctggagatca tcctctcctc gattacggaa gggatcatcg tcaccaatga
    12241 aaataacgag atccagatct tcaataaatc cgccgagaac atatttggcc tgatggcctc
    12301 gcaggtaatt ggcaaaccgg tagatacggt tattaaaaat acgcggatta atcaggtatt
    12361 gctgacaaaa tccgccgaaa ttcgggaaat tatggatttg gacaacacgt ccatcattac
    12421 cagtcgggtg ccaatatttc gtggtacgca gtgtattggg gtggtgtgtt cgtttaccga
    12481 tacgccgcaa atccagaaag tggaacggat tattcgcggc aaactgatga aaaaaggctt
    12541 caccgcccgg taccggtttg agcatatcct cacgcaaaat aagcacatgg agtcggtgat
    12601 tcatctggca aaaatttatg ccaataccca ctccccggtg atgatttatg gggaatccgg
    12661 caccggtaaa gagttattcg cgcagagtat tcacaaccag agtcaatgcc accgtggccc
    12721 atttgtcgcc attaactgtg ccgccattcc ggaaaccctg ctggaaagcg aactgtttgg
    12781 ctatgaaggg ggctctttta ccggtgcgcg gcgcgaagga aaagaaggcc tgattgaact
    12841 ggcgcaccag ggaaccttat ttctcgatga aattggtgag ctgccgctac ctattcagag
    12901 ccgcctgtta cgcgtcttac aggagtacga aattatgcgc gtggggggaa aggacattat
    12961 ccctgtcgat gtgcggatta ttggtgccac taaccgatcg ctggaaaaaa tggcggagga
    13021 aggctcgttt cgcggcgacc tctactatcg gttgaacgtc ttgccgctaa tggtgcctcc
    13081 gctacgggaa agagagggcg atgtccgttt tctggctgag cacttcctcg aaaaatcggg
    13141 ctgcgggcat aaactagcgc agttcatcgc gctgttttcc acctacaact ggcccggaaa
    13201 cgtgcgtgaa ctgaatttta ttattgagcg actggcacta ttatccgccg gatttcccaa
    13261 cgcttcgctg tttgaaatat taaatctgac cggctttaac gcgctaaaat cctgcaacac
    13321 gtcacagacg aatgggagga tcgtcgcgga tctgagtaaa ggacaactga aagccgtcat
    13381 taaagatctt gagcgacaga ttattgcctt ctacatggat ctttttgaac aggatcaaga
    13441 gaaagtcgcc gcccatctgg ggatcagcaa aatgagttta tggcgtaaat acaatcagat
    13501 ggatcgctaa gatttcatct catcgttgct ttaaatcttc cagtatcatc ggcggatttt
    13561 gccggtgtaa catcacgtgg caagcgatcg ggtaattcat tcatttgatg agatccatgt
    13621 aatccggggc tttgcttgtc tcattgacag gactccgtga ccccgccagt gtatccctcc
    13681 tttaccgctt gatcaggccg tattttgcga gcatcatcgc aattatttcc cgagtaatga
    13741 aatcaatggg ttccagtcaa atgggttcca gtcaaatgtt gcaacaggca attgacgacc
    13801 tggcgcaatg gcgagcagtg cgcgccgcat aagccattac tgctgttatg cgtactggcg
    13861 ggttatcggc aaggtcatgg gcgtctgttt aattacgctt cggaagatcg cgacaaccta
    13921 catagtctgc tggagcgatt tggcccccaa cgagcccagt accgaccgga tatgcctttc
    13981 tggcgcttac agggggacgg tttctggcaa ctgcaaaacg cggagcgctg ttccacattt
    14041 ggcagcagtc gacgacctcc cgcaggtgag ctggttgagc ataacgttgc tggcggcttt
    14101 gataagcaac attacgacca actgataaaa agtaagagcc tgattaattc cctcgcccga
    14161 caaatcctcg aagcccattt caccgagagc attcaggaag aactcgccga tgaactagga
    14221 tttgagctct tgttgctacg taagcagcgc gatcccctgt tccgccaaca ggtactgcga
    14281 gcctacaact acgaatgcgc catttgcggc ttcaatatgc gccacgacaa tacttctgtc
    14341 gcgctggagg cggcctatat caaatggaag cagtacggtg gcccttgcga aattcctaat
    14401 ggtctggccc tctgcgcaat ttatcataaa gcttttgata agggatccat tggtttagat
    14461 gagagtatgc gagtacaggt ttcagaagcg gtcaacggta gcgggatcgt cggtaggttg
    14521 ttttgggatt ttcaggggaa gagcatttct ctgccgcaag taaaagcata ctacccgcag
    14581 gacgaattcg tggagtggca taggagggag gtgttcagga gaggatgaag atctggcttg
    14641 ctcaggaaac atctgaagtg gtcaacaaaa actggccacc gagttagagt ttttccagta
    14701 tcgattttcc gattcgtttg ggggtaaccc accgttatat tcgtgcggtc ttagtgcgct
    14761 gtaatatcca acgatatagt ccgttatggc gtgagctgcc tcgctgaagc ttacgtaacc
    14821 caccaccggc atccattcgt tcttcagact cctgaagaag cgttccattg ggctgttatc
    14881 ccagcagttt ccgcgccggc tcatactctg tctgatctgg tatcgccaca ataactgccg
    14941 gaactgcctg ctcgtataat gactgccctg atcgctgtgg aacatcaccc cgccgggctt
    15001 accacgggtt tcccatgcca tttccagcgc tttcatggtg agcctgctgt ccggcgagaa
    15061 cgacatggcc cagcccactg gttttcttgc gaacaggtcg agaacaacgg cgaggtacgc
    15121 ccagcgctta cccgtcc
//
LOCUS       DAZFZC010000106        15071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFZC010000106 DAZFZC010000000
VERSION     DAZFZC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.107"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..833)
                     /locus_tag="ACHKXP_004016"
     CDS             complement(<1..833)
                     /locus_tag="ACHKXP_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO7648923.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLVPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQNTENSTGKISPVIAATHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     K"
     gene            complement(926..1117)
                     /gene="ydfD"
                     /locus_tag="ACHKXP_004017"
     CDS             complement(926..1117)
                     /gene="ydfD"
                     /locus_tag="ACHKXP_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO7648924.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(1114..1302)
                     /gene="dicB"
                     /locus_tag="ACHKXP_004018"
                     /gene_synonym="ftsT"
     CDS             complement(1114..1302)
                     /gene="dicB"
                     /locus_tag="ACHKXP_004018"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7648925.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(2263..2364)
                     /gene="ydfB"
                     /locus_tag="ACHKXP_004019"
                     /pseudo
     CDS             complement(2263..2364)
                     /gene="ydfB"
                     /locus_tag="ACHKXP_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            complement(2366..2521)
                     /gene="ydfA"
                     /locus_tag="ACHKXP_004020"
     CDS             complement(2366..2521)
                     /gene="ydfA"
                     /locus_tag="ACHKXP_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7648926.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2722..3129)
                     /locus_tag="ACHKXP_004021"
     CDS             complement(2722..3129)
                     /locus_tag="ACHKXP_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7648927.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            3207..3434
                     /locus_tag="ACHKXP_004022"
     CDS             3207..3434
                     /locus_tag="ACHKXP_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7648928.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3418..3939
                     /locus_tag="ACHKXP_004023"
     CDS             3418..3939
                     /locus_tag="ACHKXP_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7648929.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            3920..4815
                     /locus_tag="ACHKXP_004024"
                     /pseudo
     CDS             3920..4815
                     /locus_tag="ACHKXP_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4856..5278
                     /locus_tag="ACHKXP_004025"
     CDS             4856..5278
                     /locus_tag="ACHKXP_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7648930.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSSPDG
                     EIRRYDSRLNIICRECRKSEVMQRILAFYQGNFQDVAQ"
     gene            5275..5764
                     /locus_tag="ACHKXP_004026"
                     /pseudo
     CDS             5275..5764
                     /locus_tag="ACHKXP_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145667.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            5894..6076
                     /locus_tag="ACHKXP_004027"
                     /pseudo
     CDS             5894..6076
                     /locus_tag="ACHKXP_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6242..6663
                     /locus_tag="ACHKXP_004028"
                     /pseudo
     CDS             6242..6663
                     /locus_tag="ACHKXP_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7319..8389
                     /locus_tag="ACHKXP_004029"
                     /pseudo
     CDS             7319..8389
                     /locus_tag="ACHKXP_004029"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            8462..8611
                     /gene="ydfW"
                     /locus_tag="ACHKXP_004030"
     CDS             8462..8611
                     /gene="ydfW"
                     /locus_tag="ACHKXP_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO7648931.1"
                     /translation="MDNARIDLRSKYYVKPKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKKD"
     gene            complement(9048..9380)
                     /gene="flxA"
                     /locus_tag="ACHKXP_004031"
     CDS             complement(9048..9380)
                     /gene="flxA"
                     /locus_tag="ACHKXP_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO7648932.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLWAQLEQLLRQQAEKKNEDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(9583..9888)
                     /gene="ydfV"
                     /locus_tag="ACHKXP_004032"
     CDS             complement(9583..9888)
                     /gene="ydfV"
                     /locus_tag="ACHKXP_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO7648933.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            9913..10152
                     /gene="relB"
                     /locus_tag="ACHKXP_004033"
     CDS             9913..10152
                     /gene="relB"
                     /locus_tag="ACHKXP_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO7648934.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            10152..10439
                     /gene="relE"
                     /locus_tag="ACHKXP_004034"
     CDS             10152..10439
                     /gene="relE"
                     /locus_tag="ACHKXP_004034"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO7648935.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            10511..10666
                     /gene="hokD"
                     /locus_tag="ACHKXP_004035"
     CDS             10511..10666
                     /gene="hokD"
                     /locus_tag="ACHKXP_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7648936.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            10883..11134
                     /gene="rem"
                     /locus_tag="ACHKXP_004036"
     CDS             10883..11134
                     /gene="rem"
                     /locus_tag="ACHKXP_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO7648937.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            11201..11479
                     /locus_tag="ACHKXP_004037"
     CDS             11201..11479
                     /locus_tag="ACHKXP_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648938.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            11481..12530
                     /gene="ydfU"
                     /locus_tag="ACHKXP_004038"
     CDS             11481..12530
                     /gene="ydfU"
                     /locus_tag="ACHKXP_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7648939.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            12544..13296
                     /gene="ydfT"
                     /locus_tag="ACHKXP_004039"
     CDS             12544..13296
                     /gene="ydfT"
                     /locus_tag="ACHKXP_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7648940.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(13574..13663)
                     /locus_tag="ACHKXP_004040"
     CDS             complement(13574..13663)
                     /locus_tag="ACHKXP_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7648941.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(13718..13930)
                     /gene="cspF"
                     /locus_tag="ACHKXP_004041"
     CDS             complement(13718..13930)
                     /gene="cspF"
                     /locus_tag="ACHKXP_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO7648942.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            14231..14446
                     /gene="cspB"
                     /locus_tag="ACHKXP_004042"
     CDS             14231..14446
                     /gene="cspB"
                     /locus_tag="ACHKXP_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO7648943.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
     gene            complement(14810..14980)
                     /gene="ynfU"
                     /locus_tag="ACHKXP_004043"
     CDS             complement(14810..14980)
                     /gene="ynfU"
                     /locus_tag="ACHKXP_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO7648944.1"
                     /translation="MSERKNSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT         4101 a         3153 c         3729 g         4088 t
ORIGIN      
        1 ttttttgccc agcgatggat gctgccgtca atgtttccgg catccacatc accaggccag
       61 agagcgtagg ccagttcgtc atccagtgtt ttccatgtct gcttgtattc gcgatgagtg
      121 gcagcaatga ccgggctgat ttttcctgtt gaattttcag tgttctgttg attggctctg
      181 gcgcgggcga gatcaacaac agacgtgtat tttccggttt ccttgcgttc accttcgcga
      241 cgttttttcc agatgcgcat ctctgcctga atttcgggcc atttggcacc aggcttacat
      301 ttatgcttaa cccacccgat ggcatgcagc ttaagctccg gatacatggc gttaacttct
      361 ggcattttca tcaacgcttc aacgatatgt ccgtcgaatg ttgccatgtc ttcctgcaac
      421 aattcctgcg cgctaatcac catatcaacg gtgatgtttt cacatgtgtc gaacttaacc
      481 atgacagcgt tctgtacttc agggaccagc ttgtcaaaag tgacgttcat cggatctgat
      541 tcagtctcaa ccgggacaaa ggaagcagac tcctcatccc agcggttttc ctgcatatat
      601 tcagcatccc atgaatcgag ggcagggcgg ggtatgccag gtttatcctc gcagacaata
      661 aatttataag cgcagtcctg agcagccgga taatgttcca ggaattgcca gtgaaatttt
      721 gctcgagcac ggcgttcgtc gccagcttca atggctgtgg ctacagccac agcgccttct
      781 tcccttgttg ccagttcgtc aggaatagcg gcgcaaataa agactttact catttgtttt
      841 aacctcatga cagatttaag gatgaacaaa tccctgccat tgctggcata taagaatgaa
      901 accggatatt tattacggaa ctgttttaaa gacctgccgg gatttcgata ttatcctggt
      961 gaataacttt atcgaccggg taacagttac cgggaatttt ctgttcggtt gctgcagtca
     1021 tacactcctg cattgtcctg tgaacactga ctgcaatatc aactggctct ccggaaacaa
     1081 gaaaaactgt cagaacaagc acaaatgctg aattcattgt gcacatcctt ttggcatcag
     1141 acgtaaacga gccagcattg aaacaatgca tattttattt aatagctccc gttcttgttt
     1201 tctcttgtta atggcatctt cagtaaatac tgggttactg atagtgacac caatttcaaa
     1261 acaaccttca gacgtattaa cgtttggtaa taacgttttc attatcgcgt cctcaacaat
     1321 gaattttgtg atgcagtgcc tggtgcctcc aggtgacgtt aaccagttaa caattaacgc
     1381 cggatacaga gaatccaccc ataacactgt ttttggtttt aactgttccg cgtgcgctca
     1441 gccgcattca ccacatcaca aaattcactt taaaaagggc ggcagagcag tcacggagta
     1501 aaactgatac cgccaaacgt caccagaaaa ttgataacag agggcgttgc agcggggttg
     1561 tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag gaataacccc
     1621 gccatactta ccgccgcgcc attttgcggg ttgccacaac cggaagcgca cggtcgaatt
     1681 aaatttaacg acaccgtaca gtgagacgaa cttcgccgtg cgctttcgtg ttgtgtgcct
     1741 gcttttaacc acgtcaggcg aggtggtatc cttaaaatca ccacagtttt aaggattcat
     1801 taagcaatgt cgcaaccacc aataaatccg cttaagaaca tgaaaattga ttactggtat
     1861 aaagcgctta cagttgttgg cgctgcgttg tttgtcttta atggaacgtc tttttttgac
     1921 agatatcccg ttgttccatt gggttttttg tcctccggca tcttttttat tggtttgggg
     1981 gagtggatta atcaccctct caaagtgaga tttattggtc ctggagtttg gactcgtgga
     2041 tataatcgtt cttcgtgcgc actcggtatc atcttcgaca tacttggttg tttcctgatt
     2101 gttacaggag tcgtcaagtt cttctgatgt aaaaccgcaa atggggcacg taacgggaat
     2161 tttgaaaagc gtttctccgg gttccagaac aaaattttct gcggtctgat tttgcttctc
     2221 atatttgtgc tccgcgtcat tgtgagagca cattcttatt ctgagtgcct gtttaaactc
     2281 actgaagctg agagcttctt cgccttcggc aaggtcttcg aagtattctt cgtaagcctt
     2341 ttccatgatt gtgtcgaaat ccatatcact cacctgagtt tctttccagc cagcgacggg
     2401 caccattttc ggttttaaac gttttgcttt tggtatacgt catcgcggtg aacgtgccgt
     2461 cctggttggg gaacacgcca cataccagag attcgctgtt gccaagatcg atagtatcca
     2521 tgctgacctc atttcccctt aacgccgggg tagcggggta gcggaactgt ttgctgagaa
     2581 caccgtgcgg tgtcttgatg gaggtaattt agttttctca ttatttttcg tcaaggtttt
     2641 ttggtgagaa aactcaagta ttggcgcaag ataaagccaa tacattgaaa tgtaaggctt
     2701 taaaattttg tgaagggatg attattgatg cttgttgcgt ttgcgagcct ctaatagctc
     2761 ggtgaacagg cgattaaaat tctcaacgcg ggcgcggagt tcgctgagtt gtgcttgttg
     2821 ctctgattta ggaagtgcgc gatacaaacg cagcatctct aactcatctt ccgataagtc
     2881 taaggcacta tcgagtgaaa cagggggagc tggtgttttg tcctcgtcgc caaacagtat
     2941 ccaggttggt gaacattgca atacctcggc gaggcgatgc aaattttgcc cgcgcggggc
     3001 tgtatggtca ctttcccata atgaaattga tgagccagat acgccagcag ctttgcttaa
     3061 accgttttga cttaagccta cctgcttacg tctttctcta atgcgttgac ctaaagtttt
     3121 ctcgttcata tttagatatc ttaataaccc ttgacttgag attccttgag tgattagcat
     3181 tgagaaaact caatattgga ggtgcgatgt ttaaatcaga cgtaattaat ttttacggta
     3241 cgaaagccaa agtagcgaaa gctgctggcg ttgacccatc tgctgtttct caatggcaag
     3301 agctggttcc tgaaggtcgc gcgatgcgtc tacaggaggc atctggcggc gagcttctgt
     3361 atgatcccaa ggtttatgac gaatatcgta agacgaagcg ggcggggcgg ttgaacaatg
     3421 aaaatcactc ctgaacaggc tcgtgaggct ctggatgcct ggatatgtcg accaggaatg
     3481 acacaggagc aggcgacgat attaatcact gaagcattct gggctttgaa agagcgcccg
     3541 aacatcgatg ttcagcgtgt cacagatgaa ggtggcgcgg ttgatcagcg agcgcttggc
     3601 gttaatcgag tgaagatatt cgaacgctgg aaggctatcg acaccaggga taagcgtgaa
     3661 aagttcacgg cgctagtgcc tgcaattatg gaggctattc ggattaatga tttcaggttg
     3721 tatcgtgaaa ttagtgacgg aaaaagcatc acgtacatga tcgccgggtt aaacaaagaa
     3781 tatggcgatg tggtggagtc cggactgctt tttgctgatc ctgccgtagt ggatcgtgaa
     3841 actgacgaac ttatagaaaa agcaattgct ttcaaacttg cgtatcgaca gcaataccaa
     3901 caaaaagctg gatggaatta tgagccttct ttttgctgaa cgcccactgg ttataaacac
     3961 acagcttgcg atgaagattg gcttaaatga agccattgtg ttgcagcagt tgcattactg
     4021 gttgagagat accaattccg gcatggaatg tgatggtgtt cgctggattt acaacacaac
     4081 ggaacaatgg ctggaacagt tcccattctg gtcagagtca acgttaaagc gcgcgtttgc
     4141 aagtctgaaa acgctggggc ttttgcgttg tgaaaagctc aataaatcaa agcgcgatat
     4201 gaccaatttc tacacgatca actacgggag cgagctttta gatggtgaca aattgagcga
     4261 atccatcggt ttaaaatgcg ccgctccatc aggtcaaaat gacctgatga tcttacagag
     4321 aatacaacag agattactac agagaataaa aacacttttc gtccggaagc ttcgcaaccg
     4381 gacccgcaga ccgctgaaca ggatttttta acccggaacc ccgacgcggt tgtgtttagt
     4441 gcgaaaaaac gccagtgggg tagcagggag gatttggcgt gtgcgcagtg gatttggggg
     4501 cggatcgtga acctttacga acaggctgcc agcgacgatg gagagatcat gcgaccaaaa
     4561 gagcctaact ggacagcctg ggccaatgac gtgcgcacaa tgcggatgct ggatggcaga
     4621 agccacagac aaatttgcga aatgtttggt cgggtacagc gagatcaatt ctgggtaaaa
     4681 aacatcatga gcccgtcaaa actccgcgaa aaatgggacg aactggtcat ccgcctggga
     4741 cgttcacctg tacagcgttg tgtgaatcat atttctgaac cggatacaga aattccgcct
     4801 ggtttcaggg gataagtgtt gattttaggg cataaagtaa tgttcaggag gacttgtggc
     4861 aaaagttttt acacaagaag agcgggaaaa aattaaaggg caggtggtgg aactcgtacg
     4921 ccagagtggg cgcgagacgt tacgacaact ggaagctaaa actggggcaa caagatatct
     4981 gatgagcgtt ctggccagag agctggttgc cagtggcgat gtatacagct ctggttacgg
     5041 gttattcccg tctgaacagg cgcgtaagga ctggcaaaac gcccgcaaaa aactctcgag
     5101 ggcaaaggtg aagaaacctg ctgtggttga tccggacctt atctggtcat cacctgacgg
     5161 agaaatacgt cgctacgaca gtcgcctaaa cataatctgt cgcgagtgcc ggaagagcga
     5221 agttatgcag cgcatactgg ctttctatca gggtaatttt caggacgtgg cgcagtgagt
     5281 gcaccggcaa ccattcttga tatgtgctgt ggcagccgca tgttctggtt cgataagtct
     5341 gacgaacggg cgatattcag cgatatcaga aaagaaggat acacattacg caatgggaga
     5401 cgcttgatta tcagccctga cattatcgca gattttcgtg cattatcatt tgcagacgca
     5461 tctttttcga tggttgtatt agaccctccg catcttgaga gtgttggtga taacgcctgg
     5521 atgggaaaga aatatggacg gctgaataaa gatgcctggc gtgatgattc gcgacagaga
     5581 tttaaagaag cctttcgggt gttgaggccg cacggcgttc tgatttttta atggaatgaa
     5641 acgcaaatac cggtaagcca gattctggca ctgacagaca gaaaacctgt tatctgtcaa
     5701 cgaacaggga aaaaacgata aaacccactg gattattttt atgaaagagg catccagtga
     5761 gtagattcgt aaggctacag atacgtatat ctgaataatt aaattcagtt ctgtaaataa
     5821 aatttaatcc ttaaccggat ggatttctgc accctcagaa catcaggagg ccgcccgaaa
     5881 gggcggtagt gaaatgcgaa agttcaaaat tattattgaa acgggaatag ctggtggaga
     5941 ttttgaggat gtattcgaag tggacgatga cgcaacacct gaggaaattc atgacggagc
     6001 ataagaaatt ttctttaact actgcaatta ctcataccac gaaataaaag acgatgagga
     6061 agaacaaagt ggctgatttt tgttcagcta aatataacat cagttttgaa gagcgggatg
     6121 aactattaat ggactatggt gagttacgcg gtggaagtgc tgctgatgtc gaatcctagc
     6181 gtgatgacta tgaagcggga aaaactccgg tcgaagcata ttgtgatgag tggggcgatg
     6241 aatgagcgag attaattatc aggaagggca tgaaacggcg gggcaagcaa aaccagtggc
     6301 atggcgatat cgctacgtga aaaaaggcgt tacggacttt caggggaagt agtggtctgg
     6361 tgactggaaa tatgtaccga aaaaagagga ttgtaacgac aggccgaact atcaaattca
     6421 ggccttattc actgcctcac cagtcccagt tacatcagaa gaactggtta aagctgtgca
     6481 cttttatgaa caactaaaac gcgaaaatcc accaacatcc ggcaactaga ttaatggatt
     6541 aactatgtcg gttaaacgac cagccaactg aaaaagcgga aacctgatta caggttgcca
     6601 gataaggcaa tgagctacct ggcgcggaac ggactgataa gtatggggaa tgttttacga
     6661 tgaaccttta gactaaagag tttgtaacgc tatgtaagtg attttttctg gtttagatat
     6721 ttatatgtcc ggccaaattg aggtgtgttt aaatgtaatt gcacattgat tgtaggagga
     6781 ataatgaaaa acgcattgca gtttttgttt gttgcgttct ggttgttcgc atcatgtatg
     6841 cccatcatct tcacagcaag gtatatggaa aaagttgatg ttttgatatt aatatttgga
     6901 tatataaatg cccttttttt aggggtgttc atggcggtca tgtgcattga atactggcgg
     6961 taaatacagc gaactccatt ggtttagttg gatatttact gtgctggaca aaaacggttt
     7021 gcggggaaat cttagttaag tagaatgact gcgggtgctt gaggctatct gcctcgggca
     7081 tggacaccaa cggcagatag agaaaagccc cagttaacat tacgcgtcct gcaagacgct
     7141 taacattaat ctgaggccaa tttcatgcta gacacatgta ggttagcctc ttacgtgccg
     7201 aaaggcacgg agaagcaggc tattgttaac accaagctgt aatgtcccct ttgaaccatt
     7261 ctaaaatgtc cccagacaat tctctggggg atttttcatg atcaaagaga ctgttacgat
     7321 gagtcataag gaactccacc gacttcagat tattcaggaa caagctgcgg cacgcattgg
     7381 catttctatt cggcaggtta aacgtctggt gcaacggtat agaaatgaag ggccttctgg
     7441 tctggtttcc caccgacgtg gaaagcgtcc taataattcc ttttctactg aattcagagc
     7501 aacagtaatt tcactcctca aaggccgtta cgctgatttt ggacctacgt ttgcgtgcga
     7561 aaaattgcgc gagatacacg gtttatcttt atccgttgaa actctcagaa agtgggtgat
     7621 agaagagggg ttatggcgtg aacgccgtcg taaaattgcc cgtatatatc aacgccgcat
     7681 gcgacgacca tcttacggtg aactgatcca gattgatggc tcacctcatg actggtttga
     7741 aaatcgaggc cccagatgta cactgatcgt tttcattgat gatgccacca gtgcgttgat
     7801 ggcgttgcgt tttgtgcctg ctgaaacaac ccgggcttac atggaaaccc tccggggtta
     7861 ccttaatgat catggcgtac cgctcgctct ctactctgat agacacagta tattcagggt
     7921 aaataaccca gagcgggaag gtgagctgac ccagttcact cgtgcgataa agacactggg
     7981 catcgagcca atccatgcca acagcccgca ggcaaaaggg cgggtagagc gcgccaatca
     8041 gacactacag gacaggctgg tcaaagaaat gcggcttcag aatatcagtg atattgaaac
     8101 agcaaatgca tggttgccga cctttattga agcctataac aaccggttcg ctacgtcgcc
     8161 tcgtactact gataatgctc atcttgatgt gcaccattct gaagaggaac tgggttatat
     8221 cttcagccta caggcgaagc gcgttctgtc taaaaatctc actttccagt acaaaagcag
     8281 tgcgtttcag gtacgcagtg agggccgggg atatcgactt aggcattcgg ttgttactgt
     8341 atgcgagaac tttgacggtg aaattaacgt tctgtatgac gggaaagcgc tgggctggga
     8401 aaagtatgtt gatggcccgg agcctatacc actggatgat gaaaagagtg tccatgaacg
     8461 agtggataat gcccgtattg atttacgctc aaaatactat gttaaaccta aagctgacca
     8521 tccctggctt acgcgccgaa cgcaaagtca tcagcaagtt aagcccccga agttacctaa
     8581 aaagaagcct gatcccgata aaaaagattg aaaccaagat cgattcggtt gagtgcatat
     8641 ccattcatag ggtagattct taagtcgcgt ttctggtgtt cattttcggg tggtttgtta
     8701 cttgttttac cggggatatg ccagaaacgc gctgagtcag tctgggcggt gtgcgtaatg
     8761 aggcgttatg gtaaatagcc tatgctaatg tccgctaaga gcaagaagcg gaagttggca
     8821 gttttgtgga ctgtccccac aaaagtgact acagaaatag ttgcaattca taattgatca
     8881 tgggttgtca gttaaactcg tggcgattta aatagactaa ttgggagtgc gtccattact
     8941 tatatcttgt aatgttaact atcagaaatg atacaaagat aatatgtctt taaagaaaag
     9001 gctgatggcg aaaagtggcc cgatgagggc cacaatacgg ctgtcactta gacgtaaata
     9061 tcaatggtgc cagcggtatt tgtatcgtct tttttctctt cttttttatc aggctgaact
     9121 gtcgcgtctt cattcttttt ctctgcctgc tgccttaaca actgctccag ttgagcccag
     9181 aggctttcaa tttgcttctg taccaatgca gccatttctt ttttctgctg tgtcgtcatc
     9241 ccctcttccg atgagatttt cccaagcttt tcagtcagca cctgaatttg tcttgtgatt
     9301 ttggctattt ctgatgttcc ttccggggcg gagttgtttg aaataacggt tgaggtattt
     9361 ccctgaattg tgacagacat agatttctcc ttttaaaaaa gcactatcgg catgtacaaa
     9421 aaaatcttta atcgtatttc ttgtgtcatt aattgtttga tgttcagatt gttttcctcg
     9481 cgggctggcg cgcctcagaa agtaaagctt gttgacaggg gtaaacgttc ggcaataatt
     9541 ttctgccgca tgcgggtgtt gcataaaacg tgttacgttc ctttatcgac aggtcaggtc
     9601 accgctcacc cgccgacgag aaagcaacac tgacatgcta aagcaaaaaa tagatgaata
     9661 agttgagttg tgcatatgta gcctgaccgt cacaaagtat atggtgtctg taccagtaag
     9721 atgatggccg gactctttaa aaacgagctg acctgcacaa tacaggatgg acttagcaat
     9781 ggctgctcct ggcacaaagc ggacagtgat caccgttctt acgactactt tctgacttcc
     9841 ttcgtgactt gccctaagca tgttgtagtg cgatacttgt aatgacattt gtaattacaa
     9901 gaggtgtaag acatgggtag cattaacctg cgtattgacg atgaacttaa agcgcgttct
     9961 tacgccgcgc ttgaaaaaat gggtgtaact ccttctgaag cgcttcgtct catgctcgag
    10021 tatatcgctg acaatgaacg cttgccgttc aaacagacac tcctgagtga tgaagatgct
    10081 gaacttgtgg agatagtgaa agaacggctt cgtaatccta agccagtacg tgtgacgctg
    10141 gatgaactct gatggcgtat tttctggatt ttgacgagcg ggcactaaag gaatggcgaa
    10201 agctgggctc gacggtacgt gaacagttga aaaagaagct ggttgaagta cttgagtcac
    10261 cccggattga agcaaacaag ctccgtggta tgcctgattg ttacaagatt aagctccggt
    10321 cttcaggcta tcgccttgta taccaggtta tagacgagaa agttgtcgtt ttcgtgattt
    10381 ctgttgggaa aagagaacgc tcggaagtat atagcgaggc ggtcaaacgc attctctgaa
    10441 ccaaagcatg acatctctgt ttcgcaccga aggtgacact tctgctttgc gttgacagga
    10501 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac
    10561 cgtcatagtg acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg
    10621 ccagacggag gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg
    10681 gagaaatccc tcgccacctc tgatgtggca ggcatcctca acgcacccgc acttaacccg
    10741 cttcggcggg tttttgtttt tattttcaac gcgtttgaag ttctggacgg tgccggaata
    10801 gaatcaaaaa tacttaagta gcgcgcaggg ataagaggga tggtccctta aaggggagag
    10861 ctaattatcc ggaaggattc tgatgatgaa catcgaagaa ctgcgtaaaa ttttttgtga
    10921 agatggcctc tatgctgtgt gcgttgaaaa tggaaatctt gttagtcatt accgcattat
    10981 gtgtttgcga aagaatgggg ctgcgttaat taattttgtg gatgctcggg tcacggacgg
    11041 atttatcttg cgcgaaggtg agtttgtcac ttcattacag gcattgaaag agatcggaat
    11101 aaaagctggc ttttctgctt tttcaggaga ataaactcat ctacaatctt gcgcggggct
    11161 gaactcccgc tgagtaacac cgtgccaccg gagaaaaccg atggcacgca acgcaaaata
    11221 ttacaattct gataattcgc ccgttcttgc ctgcacgcac gggcggtatt ctcacgcatt
    11281 caagtctgaa tggttccagc accctccatg cactgcagaa caggccgaat ggctgattca
    11341 ttcttaccgc aggcgcgggt tcgaggttaa gaaagctctc agtctcgact atcggcactg
    11401 gataatctct gtcaggctgc cttattccga acgcccacca cgtgcgtccc gcactttcca
    11461 gcaacggatc tggaggtaac gtgcgggtat tacttagacc tgttctggtg cctgagcttg
    11521 ggctggtggt ccttaagccg ggccgtgaat ccatacagat atttcataat cctcgagtgc
    11581 tggtggaacc ggaaccaaaa agcatgcgta atctgccatc cggagtcgtt cctgccgttc
    11641 gccagccgct ggcggaagac aaaacattgc tgccgttttt tagtaacgaa cgggtgattc
    11701 gtgctgctgg cggcgttggc gcattgtccg actggctatt acgtcatgtt acatcctgcc
    11761 agtggcctaa tggcgattac catcacactg aaacagtcat tcaccgttat ggtaccggcg
    11821 caatggtgtt gtgctggcac tgcgacaacc aactgcgtga ccagacatcg gaatcactgg
    11881 agctgcttgc tcaacaaaat ctgacagcat gggtgattga cgtcatccgt cacgcaataa
    11941 gcggtacgca ggagcgggaa ttatctttgg ctgaattatc ctggtgggcg gtctgcaatc
    12001 aggtggtgga tgcactacct gaggctgtat cgcgtcgttc gctgggatta ccagcggaaa
    12061 aaatctgctc ggtgtaccgc gaaagcgaca tcgtaccggg agagcagacc gccaccagca
    12121 tattgaaaca acgcacaaaa aatcttgcac cgttgcctta cgcccaccag caacaaaaat
    12181 caccacagga aaagacggtg gtaagcatca ccgttgatcc agagtctccg gaatctttca
    12241 tgaagctgcc taaacgtcgc cgctgggtta aggagaaata cacacgttgg gttaagacac
    12301 agccgtgtgc ttgctgcggt atgccagccg acgatccgca tcatctgatt ggtcacgggc
    12361 agggcggaat gggaacaaaa gcacatgatc tctttgtgtt gcctttgtgc agaaagcatc
    12421 acaacgagct gcatacggat acagtggcat ttgaagataa gtatggctcc caactggagc
    12481 tgatatttcg ttttatcgat cgcgcgctgg caattggcgt actggcgtaa gtggagaacg
    12541 agcatgaacc ttgaagcctt accaaaatat tactccccaa aatctccaaa attgagcgat
    12601 gacgctccag cgacaggcac cggttgttta acaattacgg atgtaatggc agcgcagggg
    12661 atggtgcagt cgaaagcacc acttgggttg gccttatttc tggcaaaagt tggtgttcag
    12721 gaccctcagt ttgcgattga aggcctgcta aattacgcga tggcactgga taacccgaca
    12781 ttgaacaaat tgagtgaaga aatccggtta cagattattc cttacctcgt gagttttgcc
    12841 tttgctgatt actccaggtc tgcggcaagt aaggctcgct gtgagcattg ttcaggtacg
    12901 ggattttata atgtattgcg cgaagtggtg aaacactaca gacgcgggga atctgtaatc
    12961 aaggaagaat gggtgaagga actatgtcag cattgccatg gtaagggcga agccagcaca
    13021 gcgtgcagag ggtgtaaggg taaagggatt gttctggatg aaaaaagaac ccggtttcat
    13081 ggcgtaccgg tatataagat ttgtgggcgt tgtaatggaa accggtttag tcgtttaccg
    13141 accacgctgg cacgacgtca tgtccagaag ctggtaccag acctgaccga ttatcagtgg
    13201 tataaggggt atgcggacgt cattggtaaa ctggtaacaa agtgctggca ggaagaagca
    13261 tacgcggaag cgcaattgag gaaggtgacg agataaatga tttttgctga agatggcgac
    13321 atgatgtttg catttttcaa aaaatatgga taaaattttt tcaacgatgg gctttgtata
    13381 cccgacgtta agaaaaagta gaaaacccgc tgatgagcgg gttttgtgct ttaaatgggg
    13441 caatggtaat gttgaatctc atcccgggac tcatgtctgt taacttatta tttagctggt
    13501 gacttggtta tttgcctgat gtttaaaatg ttttcttcca gtacaatgtc cctaaacaca
    13561 atgagtctgc ttattatatt attagcagag ctattacggc caaagtacag cataagcttt
    13621 taaagccaat caaccagtca tcaagacaga cggggttatt cataaaaact ctccatgtgt
    13681 gatccgatgg ggcctgaaat taaagcttta atatagctca tgaaaggtaa acattggcag
    13741 ctgaagggcc acgcagacca tttatccggc aaaattccac gcgtaatccg gtggtaattt
    13801 cttctgcatc gcggagattg agcgctgaaa catgaagctg gacatcgata cgaccatcgg
    13861 atggggtgat aagacccttg ccgcttttgc cgtcaaaggt tttgacaatt cctgtcattt
    13921 tacgggacaa aaaaattcct taatactgat aacttggcgc actatacaca cgttcctgaa
    13981 gaaagctata gttttttgat ggggttgaag atggctggat gtctaaaata aacattgctt
    14041 catatgttca actatgcgtt aatgattgcg tcggtttgaa gaacagacga tatacgaagt
    14101 agtttactaa agcagttctc atttcaggtg ttattcactt attccttctt tgagtctctc
    14161 caattaagta cgaagtcgtt tctgttatgc aaaccattta tgccgaaagg ctcaagttaa
    14221 ggaatgtaga atgtcaaata aaatgactgg tttagtaaaa tggtttaacg ctgataaagg
    14281 tttcggcttt atttctcctg ttgatggtag taaagatgtg tttgtgcatt tttctgcgat
    14341 tcagaatgat aattatcgaa ccttatttga aggtcaaaag gttaccttct ctatagagag
    14401 tggtgctaaa ggtcctgcag cagcaaatgt catcattact gattaaaatt catcgctcgt
    14461 ctgtatacga taacgaagaa ggctgatgcc tgagtagaga tacggacaga gtagtgaata
    14521 ttggatctct ttaataaaaa gtaaggaggt ccaatacatg aaacaatggc tagcatattt
    14581 ggcaaaatct taatcaggaa aagtatgcta accattgtgg tgaagtgcag gtttgctgca
    14641 tgaatagttt tacagcagaa gctaactgct ggcatggcaa aacaaagtgc gtaagtggat
    14701 gactcccaca aaaagcacca caatctcaaa cccgctcagg cgggtttttt attatctgct
    14761 ttaaatatat tattaaaata taaaaaatac ttgttactaa taaaatcaat caggctacag
    14821 ctttaagatt tgtctggaat actttgttgc aatgagggca gatcaaaagg gcaccttttt
    14881 gtactcttga aaaactgtgt tctgactctt gggtgcagtt tgggcaggaa catttaacga
    14941 gataattacg gcgtgatttt gagtttttac gttctgacat aggcttttcc tgtataaatg
    15001 gccgtataca gtacactaaa tatgaaaaca tttctcgtat tattatttta tatatgactt
    15061 tctttcaaaa t
//
LOCUS       DAZFZC010000107        15013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFZC010000107 DAZFZC010000000
VERSION     DAZFZC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.108"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            275..1033
                     /gene="ybfD"
                     /locus_tag="ACHKXP_004044"
                     /pseudo
     CDS             275..1033
                     /gene="ybfD"
                     /locus_tag="ACHKXP_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            1184..1693
                     /gene="ybgA"
                     /locus_tag="ACHKXP_004045"
     CDS             1184..1693
                     /gene="ybgA"
                     /locus_tag="ACHKXP_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO7648945.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            1690..3108
                     /gene="phrB"
                     /locus_tag="ACHKXP_004046"
     CDS             1690..3108
                     /gene="phrB"
                     /locus_tag="ACHKXP_004046"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO7648946.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDHEGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(3258..4739)
                     /gene="dtpD"
                     /locus_tag="ACHKXP_004047"
     CDS             complement(3258..4739)
                     /gene="dtpD"
                     /locus_tag="ACHKXP_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO7648947.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITILFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            5010..5753
                     /gene="ybgI"
                     /locus_tag="ACHKXP_004048"
     CDS             5010..5753
                     /gene="ybgI"
                     /locus_tag="ACHKXP_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO7648948.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5776..6432
                     /gene="pxpB"
                     /locus_tag="ACHKXP_004049"
     CDS             5776..6432
                     /gene="pxpB"
                     /locus_tag="ACHKXP_004049"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO7648949.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            6426..7358
                     /gene="pxpC"
                     /locus_tag="ACHKXP_004050"
     CDS             6426..7358
                     /gene="pxpC"
                     /locus_tag="ACHKXP_004050"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO7648950.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKHDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            7348..8082
                     /gene="pxpA"
                     /locus_tag="ACHKXP_004051"
     CDS             7348..8082
                     /gene="pxpA"
                     /locus_tag="ACHKXP_004051"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO7648951.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLAAAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            8118..8909
                     /gene="nei"
                     /locus_tag="ACHKXP_004052"
     CDS             8118..8909
                     /gene="nei"
                     /locus_tag="ACHKXP_004052"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO7648952.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSPLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8906..9952)
                     /gene="abrB"
                     /locus_tag="ACHKXP_004053"
     CDS             complement(8906..9952)
                     /gene="abrB"
                     /locus_tag="ACHKXP_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO7648953.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(10104..11165)
                     /gene="ybgO"
                     /locus_tag="ACHKXP_004054"
     CDS             complement(10104..11165)
                     /gene="ybgO"
                     /locus_tag="ACHKXP_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7648954.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEVIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHNMTKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            complement(11162..11893)
                     /gene="ybgP"
                     /locus_tag="ACHKXP_004055"
     CDS             complement(11162..11893)
                     /gene="ybgP"
                     /locus_tag="ACHKXP_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7648955.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTAKLPGDRE
                     TLFFYNMREIPPAPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVFPGFKTVMIAPFSTVNTNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGECRLQAVEAKK"
     gene            complement(11908..14367)
                     /gene="ybgQ"
                     /locus_tag="ACHKXP_004056"
     CDS             complement(11908..14367)
                     /gene="ybgQ"
                     /locus_tag="ACHKXP_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7648956.1"
                     /translation="MDTVNIYRLSFVSCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWQKKGDKTIPCINDSLVDKFGLKPDIRQ
                     SLPQIDRCIDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWAPPSTWKEGVAGVL
                     MDYNLFASNYRPQDGSSSTNLNAYGTTGINAGSWRLRSDYQLNNTDSEDSHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            complement(14418..14984)
                     /locus_tag="ACHKXP_004057"
     CDS             complement(14418..14984)
                     /locus_tag="ACHKXP_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7648957.1"
                     /translation="MFKGQKTLTALAISLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT         3789 a         3628 c         3960 g         3636 t
ORIGIN      
        1 ttttacgaca tttaattcaa attgttggga tggggtatgc tgggtaaaaa aaaggaggca
       61 aacaaatatc cgctaacgta gtttttcaat agctacacca tgtcacgtaa catgatgcct
      121 ggatattaac cagggaaaga tcatcagaag ttcacttttt gtactaaata attcgcattt
      181 tatgtttaaa aattgagata ttccttatta cctgaagctg ttttttattg cttatacatg
      241 atcaaatact tcttacataa ttaaggagaa caaaatggaa cttaaaaaat tgatggaaca
      301 tatttctatt atccccgatt acagacaagc ctggaaagtg gaacataaat tatcggatat
      361 tctactgttg actatttgtg ccgttatttc tggtgcagaa ggctgggaag atatagagga
      421 ttttggggaa acacatcccg attttttgaa gcaatatggt gattttgaaa atggtattcc
      481 tgttcacgat accattgcca gagttgtatc ctgtatctgt cctgcgaaat ttcatgagag
      541 ctttattaac tggatgcttg actaccattc ttcagatgat aaagacgtca tcgcaattga
      601 tggaaaaata caccggcatt cttatgacaa gagtcgccgt aagggagcga ttcatgtcat
      661 tagtgcgttc tcaacaatgc acagtctggt catcggacag atcaagacgg ataagaaatc
      721 caatgagatc acagctatcc ctgaacttct taacatgctg gatattaaag gaaaaatcat
      781 caaaaccgat gcgatgggtt gccagaaaga tattgcagag aagatacaaa aacagggagg
      841 tgattattta ttcgctgtaa aaggaaacca ggggcggcta aataaagcct tcgaggaaaa
      901 atttccgctg aaagaattaa ataatcccaa gcatgacagt tacgcaatta gtgaaaagag
      961 tcacggcaga gaagaaaccc gtcttcatat tgtttgcgat gtccctgatg agcttattga
     1021 tttcacgttt gaatagaaag ggcagctatg gttagaaact acctgacgtc agtccttgcg
     1081 gggagcaggc tttcgtaaat ttgtcctgct acaacaggat taacttcaca aatatcattt
     1141 ctcaacgtct acacttactc ctgtaaaccg ctcaggagca gtaatgaatc tacaacgatt
     1201 tgatgacagc accctaatcc gtatctttgc ccttcatgag ttacatcgac tgaaagaaca
     1261 tggcttaacg cgcggggcgc ttctcgatta tcacagccgc tataaactcg tctttctggc
     1321 gcattctcag ccggagtacc gcaaacttgg cccgttcgtg gctgatattc accagtggca
     1381 aaatctggat gactattaca accagtaccg ccaacgcgta gttgttttgc tttctcaccc
     1441 cgccaacccg cgcgatcaca ccaatgtttt gatgcacgtt cagggttatt ttcgcccgca
     1501 tattgattcc acagaacgcc agcagctggc tgcgcttatc gacagttatc gccgtggcga
     1561 gcaaccactt cttgcgccgc tgatgcgtat caaacactat atggcgcttt atcctgacgc
     1621 ctggctttca gggcagcgtt atttcgaact ttggccgcgt gtgattaact tgcgccattc
     1681 aggagtttta tgactaccca tctggtctgg tttcgccagg atttacgtct gcacgataat
     1741 ctcgcactgg ctgccgcctg ccgcaattcg tctgcacgcg tgctggcgtt gtatatcgct
     1801 acaccacgcc agtgggcgac gcataacatg tcgccgcgtc aggctgaact tatcaatgct
     1861 caactgaatg ggctacaaat agcgcttgcg gaaaaaggta ttcctttatt gttccgtgaa
     1921 gtggatgact ttgtcgccag tgttgaaata gttaaacagg tgtgcgcgga aaacagcgtt
     1981 acccacctgt tttataacta tcagtatgaa gtgaatgagc gggcgcggga tgtggaagtt
     2041 gaaagagcgc tgcgtaacgt ggtgtgtgaa ggatttgatg acagcgtgat cctgccgcct
     2101 ggcgcggtga tgaccggtaa tcacgagatg tacaaagtct ttacgccttt taagaatgcc
     2161 tggctgaaac ggctgcggga agggatgccg gagtgcgtcg ctgcgccaaa agttcgtagt
     2221 agcggatcga tagagccctc gccatccatt acgctgaatt atcctcgtca gtctttcgat
     2281 actgcgcatt ttccggtgga agaaaaagcg gcgattgcgc aattacgcca gttttgccag
     2341 aacggtgccg gagaatatga gcaacaacga gattttccgg cagtggaagg caccagccgt
     2401 ttgtcggcca gcctggcaac gggcgggtta tcgcctcgcc agtgcttgca tcgcttgttg
     2461 gctgaacagc cgcaggcgct ggacggtggg gccggtagtg tctggcttaa tgagctgatc
     2521 tggcgcgagt tttaccgtca cctgataacg tatcacccct cgttgtgtaa acatcgtcca
     2581 tttattgcct ggacggatcg tgtacagtgg cagagcaatc ccgcacattt acaggcctgg
     2641 caggaaggca aaacgggata cccgattgtt gatgccgcta tgcgtcagct taacagcact
     2701 ggctggatgc ataacaggct acggatgatt acagccagtt ttctggtgaa agatttattg
     2761 atcgactggc gcgaaggcga gcgatatttc atgtcgcagc tgattgatgg tgatttggca
     2821 gccaataacg gtggctggca gtgggccgct tcaaccggaa ccgatgcagc gccgtatttt
     2881 cgtattttca acccgacaac ccagggcgag aaatttgatc atgagggcga gtttatccgc
     2941 cagtggctac cggaactgcg cgatgtgcca gggaaagtgg tgcatgagcc gtggaagtgg
     3001 gcgcagaaag caggtgtgac gctggattat ccgcaaccga tagtcgagca caaagaagcg
     3061 agagtacaaa cgttggcagc gtatgaggcg gcgcggaagg ggaaataaca gacgcgtcag
     3121 gcaatcgagc ccagatgccg gatgcggcgt gaacgcctta tccagcctac aaatttgtgc
     3181 aaattcaata aattgcagga aatacgtagg cctgataaga cgcgtcaagc gtcgcatcag
     3241 gcatcggtgc tggcctatta agactccagc gccagcgcgc ggtttctgaa tttcagcgcc
     3301 tgatacagcc aaatcatcag taccagtcct acacacgcca gtgcgcccca ggtaatttga
     3361 tcaaatactt caatatatgc attgatggag tagttgatcg ccccggaagc atcaaacgaa
     3421 gcctgcgatg tctgatcggc aatcacgccc gccagatagt tcgcgatcgc gccagaaagc
     3481 agcatgtaga tgccggttaa tacgccggtc acaccgggga tttcaatgcg cgtaatttgc
     3541 gacatggcaa ccgggtcgat aaacagttcc gcaaagccca tcaccgccag gcctaatacc
     3601 atcagtggca gagaagagtg accatacatt gcggaccagc gggcgcttaa ggtcagaatg
     3661 cagaatccgg cgctcatcag gccaaggcca agagcaaatt tcccccagat gcgcacggta
     3721 cgattacccg cgacactctc ttttaccacc cacgccagga acacaccgca cagcataact
     3781 gcgaaggcat taatcgactg gaacatcgcg gtcggaacgg tataaccaaa catatcgcgg
     3841 ttaacgaagc ggtcgatata aaggctaatc gagctaccgc cctgttgtgc gaaggcccag
     3901 aacaacatac tgaagaaggt gagcgtcaca atcagcccca gctccttccg ctgtttctgg
     3961 ttttctgctt tgcgataaat ttttgccagt acacccaggc caataatagt cgcgacaatt
     4021 aaggcgtata ccgaccactc tttccagaac agtatggtaa tcagtgcagg cgttgccacc
     4081 agcagaacca gcagccatcc ccagttcggc aggagaaagt ttgtcgcacg cagtactttt
     4141 ttgttaacgc cgcgggtatg agtgaaatga cgattgccac ataagaaaat gaccagaccc
     4201 gcgatcatgc caaccgccgc caggccaaag cccatcgccc agctgtactc ttcctgggcg
     4261 taaccacagg cgataggtgc gataatagac cccacgttac ccgccgcata catcagcgag
     4321 aaaccgccat cacgacgcgg atcggttggc tcatacagct cgccgagcag acagcttacg
     4381 ttagatttaa acaggccata gccgcagacg ataatcgcca gggacagata gaggaatgac
     4441 ggatggatct cactggcacc cagcaccaca tgaccgatcg ccatcaacaa cgcccccagc
     4501 atcaccgcca tgcgattgcc gagaacttta tccgccaaaa agccaccgag gattggcgtg
     4561 acatacacca gcgaacagta ggcgctaaat aactcgtagg cgtgagtatc gttgtatttt
     4621 agttgattgg tgagatagag aatcagcagg gcacgcatgc cgtaaaagct aaaatattcc
     4681 cagatttgca gcgcaacgac atagtaaata gcgcgcggct gtgatgcgtg tttattcata
     4741 ataattccac aattgacggc aacgtcacgt aatagttgcg cggcgtgctt gtgtttcctt
     4801 aaagttaaaa ctttgataca gatctgatta tttatgcaat atgctgtctg attgcataaa
     4861 tatacattag ctgaagcgtg atgatataaa caatttgcgt cttttggcgt cgggtttgtt
     4921 tcttaatatg tcaaaagatt ggctatcgac gaactggcga ggtaacgcta tgttaacggt
     4981 gtagctatca gaatacggaa ggcgcgaaga tgaaaaacac cgaactggaa caactgatta
     5041 acgaaaaact gaacagcgcg gcgattagtg attacgcgcc gaatggtttg caggtggaag
     5101 gcaaagagac ggtgcaaaaa attgttaccg gtgtcaccgc cagccaggct ttgctcgatg
     5161 aggcagtgcg tctgggcgcg gatgcggtca ttgtgcatca tggctacttc tggaaaggag
     5221 agtctccggt cattcgcgga atgaagcgta accgtttaaa aacgttgctg gcgaatgata
     5281 tcaacctgta tggctggcat ttgccgcttg acgcacatcc tgagctgggc aataacgcac
     5341 agctggcggc gttactgggg atcacggtca tgggcgaaat tgagccgttg gtgccgtggg
     5401 gcgaactgac catgcctgtg ccgggactgg agctggcttc ctggattgaa gcgcgtctgg
     5461 gacgtaagcc gttatggtgt ggcgataccg gacctgaggt ggtccagcgc gtcgcctggt
     5521 gcacgggcgg cgggcaaagt tttatcgata gcgccgcgcg ttttggcgtg gatgctttta
     5581 ttactggcga agtttctgaa cagaccattc attcagcccg cgagcaggga ttgcattttt
     5641 atgctgcagg tcaccatgcc actgaacgtg gtggtattcg cgcattgagc gagtggctga
     5701 atgaaaatac cgatcttgat gtgaccttta ttgatattcc taatcctgca taacgaataa
     5761 tcagagggat cgaaagtgca acgagcgcgt tgttatctga taggtgaaac ggcggtagtg
     5821 ctggaactgg aaccgccggt gacgctggct agccagaaac ggatctggcg actggcgcag
     5881 cgtctggtgg atatgccgaa tgtggttgaa gccattcccg gcatgaacaa tatcacggtg
     5941 attttgcgta atcctgagtc gctggcgctg gatgccatag agcgtttgca acgctggtgg
     6001 gaggagagcg aggcgctgga gccggagtct cgctttattg aaattccggt ggtttacggt
     6061 ggtgcaggcg gaccggattt ggcggtggtc gcggcgcatt gcgggttgag cgaaaaacag
     6121 gttgttgaat tgcactcctc cgtggaatac gtggtctggt ttttaggttt tcaaccgggc
     6181 ttcccgtatc tcgggagttt gccggaacaa ctacacacgc cacggcgcgc tgaaccgcgc
     6241 ttactcgttc cggcaggttc tgtcgggatc ggcgggccgc agactggtgt ttatccgctg
     6301 gcaacgccgg gtggctggca gttgattggt catacctcac tcagcctgtt tgatccggcg
     6361 cgtgacgaac ccatcttatt acgtccggga gacagcgtgc gctttgtacc gcagaaggag
     6421 ggagtatgct gaagattatt cgtgcgggca tgtataccac tgtgcaggat ggcggtcgtc
     6481 acggttttcg ccagtcgggt atcagccact gcggcgcact ggatatgccc gcgttacgca
     6541 ttgctaacct actggtgggt aatgacgcca atgcccccgc gctggagatc acgctcggtc
     6601 agttaactgt tgagttcgaa actgatgggt ggtttgctct gacgggtgcc ggttgcgaag
     6661 cgcggctgga tgataatgcc gtctggaccg gctggcgatt gccgatgaaa gcaggccagc
     6721 gtttaacgct taaacgcccg cagcacggga tgcgcagtta tctggcggtc gcgggtggta
     6781 ttgatgttcc gccggtaatg gggtcatgca gcaccgatct caacgtgggg attggcgggc
     6841 tggaaggccg tttactgaag gatggtgacc gactcccgat tggcaaatcg aagcatgatt
     6901 ttatggaagc gcagggcgtt aaacagctgc tgtggggcaa ccgcattcgc gccttgccgg
     6961 ggccggaata tcatgagttc gatcgcgcct cgcaggatgc attctggcgt tcgccctggc
     7021 agcttagccc gcaaagtaac cgcatgggct atcgcttaca ggggcaaatt ttaaaacgca
     7081 ccaccgatcg cgaactgtta tctcacggtt tgttaccggg cgtggtgcag gtgccacata
     7141 acgggcagcc gattgtgttg atgaacgacg cacagaccac cggtggttac ccgcgtattg
     7201 cctgcatcat tgaggctgat atgtaccatc ttgcgcaaat tccgctcggt cagccgattc
     7261 attttgtcca gtgttcactg gaagaggcac tgaaagcgcg gcaagatcag caacgttatt
     7321 ttgaacaatt agcgtggcgg ctgcacaatg aaaattgacc tgaacgccga tctgggcgaa
     7381 ggctgcgcca gcgacgcaga gctattaacg ctggtttcct ctgccaatat tgcctgtgga
     7441 tttcatgcag gcgatgcgca aatcatgcag gcttgcgtgc gtgaagcaat aaaaaatggt
     7501 gtcgcgattg gcgctcaccc gagttttccc gacagggaaa attttggtcg cagcgccatg
     7561 cagctgccgc cagaaaccgt ttacgcccag acgctgtatc aaattggcgc gctggcaacg
     7621 attgcccgtg cgcaaggcgg cgtaatgcgt catgtcaaac cgcacggcat gttgtacaac
     7681 caggcggcga aagaagcaca actggcagcc gccatcgcca gagcggtata cgcttgcgat
     7741 ccagcattgg ttctcgtcgg gctggcagga agcgagctga ttcgtgcagg caagcaatat
     7801 ggcctgacaa cgcgcgagga agtgtttgcc gatcgcggtt atcaggctga cggctcgctg
     7861 gtgccgcgaa gccagccagg cgcgttgatt gaaaacgaag aacaggcgct ggcgcaaacg
     7921 ctggagatgg tgcaacacgg cagagtcaaa agtatcaccg gcgaatgggc aacggtcacg
     7981 gcgcaaacgg tctgcctgca tggcgacggc gagcacgcac tggcattcgc ccgccgacta
     8041 cgctctacat ttgccgaaaa ggggattgtt gtcgcagcat aaccccgctt aataaagaat
     8101 gaaaaaagga tatcaccatg cctgaaggcc cggagatccg ccgtgcagcg gataacctgg
     8161 aggcggcgat caaaggcaaa ccactaactg atgtctggtt tgccttcccg cagttaaaat
     8221 cttatcaatc accacttatc ggtcaacacg ttacccatgt ggaaacgcgt ggtaaggcgt
     8281 tgttaactca tttttccaac gacttaacgc tctacagcca taatcagctt tacggcgttt
     8341 ggcgcgtggt tgataccggc gaagagccgc agaccacgcg agtattgcgg gtaaaactgc
     8401 aaacggttga caaaaccatt ctgctttata gcgcctcgga tattgagatg ttgaccccgg
     8461 aacaactgac cacgcatccg tttttacaac gcgttggtcc cgatgtgctg gatccgaatc
     8521 tgacgccgga ggtggtaaag gaacgattat tgtcgccgcg ctttcgtaac cgtcagtttg
     8581 caggattact gctcgatcag gcgtttctgg ctgggcttgg caattatttg cgggtggaga
     8641 tcctctggca ggttgggttg actggaaatc ataaagcgaa agatctcaat gcggcgcaac
     8701 tggatgcact cgcacacgcg ttactggaga ttcctcggtt ttcttacgct acgcgggggc
     8761 aggtggatga gaataagcat catggggcgc tgtttcgctt taaggttttt catcgtgatg
     8821 gcgaactgtg cgaacgctgt ggcggcatca ttgagaaaac cacgttgtcc tcgcgcccgt
     8881 tttactggtg tccaggctgc cagcactagg ccgaccgctt cggcgcatag gttgaaataa
     8941 accgcgcaat ggctggtccc gtcagcaaaa tactgaacag gcgcagggtt tgcatcgcca
     9001 taataagcgc catatcggca ttgctccctg cggcgatgac cgccacggta tcaagcccgc
     9061 cgggactggt ggcgaggtag gcagtcataa aatcaatatg cataaaccgg gtcagtcccc
     9121 acgccatacc tgcacaaata gccagcagag caaaaatcga cagcaggatt tgcggtagcg
     9181 ggcgtagtgc ccgcagtaag atttgtttat cgaaaccaag accaatccgc cagccaattg
     9241 ccatatacgc catcgccagc agccattccg gtagttcgat ggtgatgagc tgaccagact
     9301 ggagcactgc gcccgccagc atcgggatga gcatcgtgcc ggaaggaaga cgcagcaggc
     9361 atcccactgt accggcaacg accgcgagca aaatagtaag caggagatta atgctcactg
     9421 gcgggaacca gacgatatgc tggttaaccg cttcagcgtt atcgcccagc atcatgcggg
     9481 tgaccagaac agcggccccc gcgacgaaca gcactcgcag atattgcata aacgccacca
     9541 ggcgaatatc tgcgccgtaa tcttgcgcca tagcgaccat tgccgccgcg ccgccaggag
     9601 aggagcccca ggcaccggta tttccaggca atgagctata gcgcaccaat aaccaaccca
     9661 caatggcgct ggaaagcagc gttaccagta aaattgcaag cacgatcggc cagttgacag
     9721 ccagggtggt gagaatcgaa ccagtgaggt tttgcgcaat catgcagcca agaatggcct
     9781 gggcggcgag aaaagcagag cggggaagtt gcagagtaat tccgcgcata ctaaagatga
     9841 tcccggcaat catcggccca agtaatagcg ccgccgggag atgtaccgcg aggaaaccaa
     9901 tagaaaggag aagtgatagc acacataaca ttccccactg caaaactggc atccttcgct
     9961 ccctcattat tttctctttg gtgtatcgat acagcataaa gaaagccggg ccaggaaaaa
    10021 ataacagcaa tcaaggaaaa ggggaaaatc agcaattttc tgaaagagat gccctttccg
    10081 gctgcaaagg gcatcatggc aaatcagtta tagttaatct taaaaagcac cactgtatcg
    10141 aagggcccag cctcgatggg ggtacctgca atggacgaaa gttcagctgt ataggttttt
    10201 tcgtagatca acatgtcact ggtgaaatcg gcgtattcag catatttatt gaaggtataa
    10261 ggcgaggcgt tttcatccag caaacgtagc ttcagaccat taccaatcaa caatgcttta
    10321 tcttcttcaa ccaacgtttc ttcagtataa aacgaggaac tgactttaaa tccgtcggtg
    10381 cattgatcgt tttggctctt ggtcgtttta atactgaacg ttttcgtcat gttatgtcgc
    10441 cgaatatcca gcaaattaaa tttaccaaaa tcaatcacct gtgtttccgg tgaaatagaa
    10501 aaattgacgc tgcaatccag cacgcgaatg ttttccagac cagtaatatg atatttcagg
    10561 ttttgggcgg taggatcttc gttaacgcta ccggcaccgt caaattgcac gacgatatag
    10621 tcgctaagcg tactttgata atcatgcggc ggcatttctc gtattttgac gtataaccgc
    10681 atgcgagcga gaaaggtacg cgacatgtga atattagcgg gatcgcccga acaaattttg
    10741 ttctgccatc ccatcgcgat gatctgctca ggggtataaa tgtcaatgtt cttactgtcg
    10801 atacactggt tagtgtcgat gcggcttttt ccctggttgg cgtcataatc aacgccattg
    10861 taagttacgc cgagttgata atagcgatcc tgcacccccg ggtaggggtt tacccaggcg
    10921 tagacatgct cactttcaaa gttgccgttg gtattgttgt cacaatagac gggaatttta
    10981 atatcgtcag attcccaaat cttatcgccg ggtttggcat tgcctggtac agcaaaaggt
    11041 tgaatcactt ctgatttttc taccgaacca ccggatgaac caaagtagca gttcagggca
    11101 agcgcagact tcaacggcag aaataacaga cagatgacga gcaacactcc cttgccagca
    11161 ctcatttctt cgcctccacg gcttgtaaac ggcattctcc gctgcagttg agcgtcgtca
    11221 tgcgtaacgc gccgtaatca tccatataac cgagataaaa ctggctgccg ctataacttc
    11281 cggtgtttgt gttgaccgta ctaaaaggcg caatcatcac ggttttgaag ccggggaaaa
    11341 cgcctttttc atttctcccc agataggcaa tggtcagata atacgcggta ggatttttca
    11401 gtgtcagttg gttgccctgc tggctaacct gtaactgcag ttcgaccttt tctcctgctt
    11461 tcttgcgtaa tgcagccggt cgccagaaca ccttgatacg actctgaatg gcaacttgca
    11521 gaacggcatg gccgctgttt ttttccgggg cgggaggaat ttcacgcata ttgtagaaaa
    11581 atagcgtttc gcgatcgccc ggtaatttcg cggtcgaggc ttgctttact atccgcacct
    11641 gcgttgttgc tttcggttcc agacgttgta tcggtggcag ggcaaccaga agatcatcgc
    11701 ttttctcccc tttttcattt tcgagccagg aataagcgag gtaggggagt ttatcgctac
    11761 gattatccac gcgcagactg gtagctttat cattagcgtt aaacacaata cgagtgcgat
    11821 cgggttgtag tgcagcgtgg ctgcacagac tggcaaccaa caacaaaagg ggtagtcctt
    11881 tcataaatgt cattttcatt tccttaatta atgacaaggt aaaatcaggc gatttgcggt
    11941 gtcttccata tgttcaggaa gatggagcga gcaatgctga ctatcacccc agaccacggt
    12001 aaacttttga ttttcagcga ctccgcttaa ccaggcatgt ccttcctcgc caaccatccc
    12061 cacgctaatg ccgctgtcat cctggcggat atccgctccg aggggaggaa actgaccgct
    12121 ggcgttgcga atgatgacgt taacgtcttt accggaacgg gaagccagtg atttgtaacc
    12181 tatcgcgcct tcaatccacg tttctttgat aacgttttct gcaactgtta cgccgtcggg
    12241 taagtcattc atgttcaccg ccacggtgga aggctggtaa ctggaaatca acggcaccac
    12301 cgcaatgcca aaatggttgg tgtagtcgag attgccctga accggaatat ctgccacgcc
    12361 atcggtgctg accatcaggc gtggttcatt ggtggagctg cggcgatgaa acgctgcacc
    12421 atattgggtt gcggtgaaag aaccgctcca gctgctgctg acggaactgt aatcattggc
    12481 ggcataggta ccagaaatat cccactcacc cgctgaactc aggtgctgat agttaccgct
    12541 cacctgggct ccattgtcag gacggtcgga ttgcagtccg gcagacatgc cccagctatt
    12601 acgttcatcc agcgtatcgt tccacgacat gcggtgtgtg gtgctgtggc tactgttttg
    12661 catgtcatag ccaacccgac caccgttacc gaaggggagc gaaatcgaca ggtaaatctg
    12721 gttgtcgcga tctttatctt cgtaatgggt tgtattgaat gacgtcgaaa tagagatatc
    12781 tctccagtca ccaatatcaa cattaaaacc tgccgtgatg ttggcggtcg tggaggcgtc
    12841 tgcattccac caggtttgat gtagcaggtt ggcgtaaaga ttgaggttta gtggggtaat
    12901 cggttggccc acagataagc tgatcgtctg tttttcgtcc tgcgcatcgc tgtcgttgta
    12961 tttgtgatcc agatagttgg cgtagctgtg aaaatgacga tcggagaaac gataagcggc
    13021 gagggaaatc gtgctgttag tggcatccac ttgtttgctg taattaaaac ggtagcttaa
    13081 gccccgctcg tcctgctgag tatcaaaatg gctactggcc cacgtgacat caaacgacag
    13141 cgcaccaagc cacagcatat tttgcccaat acccattgcg gcagaatggt agtcatcacc
    13201 agaaagcagc aggccgccgt acagcgaggt gtttgacagc atcccccagg aaacttcatt
    13261 gctaaaaaag gtttcatttt cagtttgatg tgacatggag ggccgtggct gacccgcggc
    13321 cagtttatag cgaacctgcc cctgacgagt caggaagggc gtcgatgctg ccgaaacctg
    13381 gaaattgttc acccgaccat cttcttccgt cactttgaca tcaagcgtgc cctgaacaga
    13441 ctgattgagg tcatcaataa taaacggtcc cggagggact tttttctggt aaatgacgcg
    13501 acctgattga ctgatcgtca ccgtggcatt ggtctgtgca ataccgctaa tttgtggggc
    13561 gtatccgcgt agctcccatg gcaacattcg atcgtcactc gccagtgccg cgccagtata
    13621 agaaaaacca tcgaaaatat tggaactgaa atcggtttcg ccgagggtta atttagagcc
    13681 taattgcggt aatggacgaa aaagataggt gcgcgatatt ccgcctgact gttcatggct
    13741 atcttcgcta tcggtgttat taagctggta atcactgcgt aagcgccatg acccggcgtt
    13801 aattccggtg gtaccgtagg cgttcaggtt agtgctgctg ctgccgtcct gtgggcggta
    13861 gttgctggca aacaggttat aatccatcag gaccccggca acaccttctt tccatgtaga
    13921 ggggggagcc cagttttctg agtgccacgc cagccaggct tgcggaatac taatattcag
    13981 ttgctgattg gcttgatcga aattgaagag catttcaggt cgggaactga aatcaataca
    14041 ccgatctatc tgtggcaagg actgacggat atctggtttt aaaccaaatt tatcgaccag
    14101 tgaatcattg atgcatggaa tggttttgtc accctttttt tgccagttga ttttttgccc
    14161 attactgatt tggttgttat tcaccgcaac gctaacaaaa tattcaccgg gagcaatgac
    14221 tcctttttcc tttaataatg aaatatcaat gcggtcgcgc attgatttat cgagaacatt
    14281 cagattaaat tcgaccgcca atgcgcaagg catcgccatg accaggcagg atacaaaaga
    14341 gagtcgataa atattcacgg tgtccatacc tgataaatat ttgaagaaag gcggcgatag
    14401 cgccgccaaa gtaatactta tttataatcc agcacgtagg tcgcattcgc tgtaactgcg
    14461 cctggcgtta caggtgtagc ctgatcaatt tgttccatcc aggcaaagaa attcagcgtt
    14521 tgttcgctgg aggtcggagc caggtcgata tctggagtag cggtgcctaa aacgatattg
    14581 ctttgatctt tattcattaa acgtacgccg acgccggtgg cttcacccgt gctggtgttg
    14641 ttgagaatcg gatctgcgcc ggtggttttc gctgagctat caaaggtgac tgcaacttta
    14701 gaaatcttgc caccggaacc gttgctggag ttttccagat cacagttgac taagcgcagg
    14761 tcgacagcaa cggcatcgct gtgatgtgac tgattaatgt ggctggtggt cacctgaccg
    14821 agttcaacct ctttatcaat atcatcctga tgtatttcac acggtgcttc aataacttca
    14881 cctttaaagt gaatttcacc ggatccttca tcagctgcat aaacaggtgc agtgaatagc
    14941 agagatatgg ccagagcggt caatgttttt tgtcctttaa acataactga gtcctttaaa
    15001 taatgagata gag
//
LOCUS       DAZFZC010000108        15011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFZC010000108 DAZFZC010000000
VERSION     DAZFZC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.109"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            327..995
                     /gene="ydcL"
                     /locus_tag="ACHKXP_004058"
     CDS             327..995
                     /gene="ydcL"
                     /locus_tag="ACHKXP_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO7648958.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1094..1519)
                     /gene="tnpA"
                     /locus_tag="ACHKXP_004059"
                     /pseudo
     CDS             complement(1094..1519)
                     /gene="tnpA"
                     /locus_tag="ACHKXP_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1527..2735
                     /gene="insQ"
                     /locus_tag="ACHKXP_004060"
     CDS             1527..2735
                     /gene="insQ"
                     /locus_tag="ACHKXP_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO7648959.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLSVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(2775..3989)
                     /gene="ydcO"
                     /locus_tag="ACHKXP_004061"
     CDS             complement(2775..3989)
                     /gene="ydcO"
                     /locus_tag="ACHKXP_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO7648960.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            4042..4578
                     /gene="sutR"
                     /locus_tag="ACHKXP_004062"
     CDS             4042..4578
                     /gene="sutR"
                     /locus_tag="ACHKXP_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO7648961.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            4651..6612
                     /gene="rlhA"
                     /locus_tag="ACHKXP_004063"
     CDS             4651..6612
                     /gene="rlhA"
                     /locus_tag="ACHKXP_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO7648962.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVVKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(6704..6934)
                     /gene="yncJ"
                     /locus_tag="ACHKXP_004064"
     CDS             complement(6704..6934)
                     /gene="yncJ"
                     /locus_tag="ACHKXP_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO7648963.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            7156..7332
                     /gene="hicA"
                     /locus_tag="ACHKXP_004065"
     CDS             7156..7332
                     /gene="hicA"
                     /locus_tag="ACHKXP_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO7648964.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            7378..7794
                     /gene="hicB"
                     /locus_tag="ACHKXP_004066"
     CDS             7378..7794
                     /gene="hicB"
                     /locus_tag="ACHKXP_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO7648965.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            7873..9282
                     /gene="ydcR"
                     /locus_tag="ACHKXP_004067"
     CDS             7873..9282
                     /gene="ydcR"
                     /locus_tag="ACHKXP_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7648966.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQTIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGHLIKEYLK"
     gene            9524..10669
                     /gene="ydcS"
                     /locus_tag="ACHKXP_004068"
     CDS             9524..10669
                     /gene="ydcS"
                     /locus_tag="ACHKXP_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO7648967.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10687..11700
                     /gene="ydcT"
                     /locus_tag="ACHKXP_004069"
     CDS             10687..11700
                     /gene="ydcT"
                     /locus_tag="ACHKXP_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7648968.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRKPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            11701..12642
                     /gene="ydcU"
                     /locus_tag="ACHKXP_004070"
     CDS             11701..12642
                     /gene="ydcU"
                     /locus_tag="ACHKXP_004070"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7648969.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTTFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            12632..13426
                     /gene="ydcV"
                     /locus_tag="ACHKXP_004071"
     CDS             12632..13426
                     /gene="ydcV"
                     /locus_tag="ACHKXP_004071"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7648970.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            13448..14872
                     /gene="patD"
                     /locus_tag="ACHKXP_004072"
     CDS             13448..14872
                     /gene="patD"
                     /locus_tag="ACHKXP_004072"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO7648971.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAACCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3739 a         3641 c         3955 g         3676 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccatcgaagg gatgtgaaat
      121 taatcacagt agtcgaagtt ttttagcagc ttaacttact gaaatttaac tactgatgat
      181 tgacttagcc cctttttcgg cattgactat gtcgtctgaa aaggggctga aaaatttatt
      241 ttcaccaaca ctttttttgc cacaacacga agcggcgctt tttgctataa cttagaaagt
      301 aatataatca tctcaggaaa ctattcatgc gtaccacatc atttgcgaaa gttgcagctt
      361 tatgcggctt attggctctg tctggttgtg cctctaaaat cacccagcca gataaatatt
      421 ctggtttttt aaacaattac tctgatttaa aagaaacaac ctcggctaca ggtaaacctg
      481 ttttacgttg ggtagacccg agttttgatc aaagcaaata tgacagcatc gtctggaacc
      541 caatcactta ttatccggta ccgaaaccgt cgacccaggt agggcagaaa gttctggata
      601 aaattttgaa ctataccaac accgaaatga aagaagcgat agcgcagcgt aaaccactgg
      661 ttaccaccgc tgggccgcgt agtctgattt tccgtggggc cattaccggt gtagatacca
      721 gcaaagaagg gctgcaattc tatgaagtgg ttcctgttgc attagtggtt gcggggacgc
      781 aaatggctac aggccaccgt accatggata ctcgcctcta ttttgaaggt gagctgattg
      841 atgcagcgac taataaaccg gttatcaaag tcgttcgtca gggcgaaggt aaagacctga
      901 ataacgaaag tacgccaatg gctttcgaaa atattaaaca agttattgat gacatggcga
      961 ccgatgccac catgtttgac gttaacaaaa agtagttcca gacgcgccat cgttagatgg
     1021 cgctttttat ccggtgcgcc gtaaaacccc atccttcagg gcggggatat aaggcgcggt
     1081 tttccacctg actaggtgtt tgctgttgtt caatgtattg gcggatgacg gatattggcg
     1141 cacctccgca gctactggca aaatagccag gactccacaa aacgcctttg tagtaatacc
     1201 tgactgcaat atctggtcga tctcgtcgca gtcacctacc cgatacgcct ttgaggctgt
     1261 ttaccagact ggatatcgcc agtttgggag gatagttaat taacaaatgg acgtgatctg
     1321 gttcgccatc catttcaacc agttcagctt caaaatcagc acatacattt gaaaagtaag
     1381 tgcgtagttt ttctgtcgcg tcatggtcaa aaatctggcg tcggtatctg gtgacaaaga
     1441 gcaggtgaac atgcatcagg aaaacacaat gccttccacg ccggatatcg gtttcttttt
     1501 tcacagacca aagtatcatt tgacctgtga aacgattaca ggcatttaaa ttccagttaa
     1561 gaccaggtgg tcaacaggag tgtgaaatga ggcgcttcgc aggcgcatgt cgtttcgttt
     1621 tcaatcgtgc tctggcacgt cagaatgaga atcatgaggc cggtaataaa tacatccctt
     1681 acgggaaaat ggcttcctgg ctggttgagt ggaaaaatgc cactgaaacg caatggctta
     1741 aagattctcc ctcacagcca ttgcaacagt cactgaaaga ccttgagcgg gcttacaaaa
     1801 acttcttccg gaagcgggct gcttttcccc gattcaaaaa gcggggacag aatgatgcat
     1861 tccgctaccc gcagggcgtt aagctcgatc aggaaaacag ccgtattttt ctgccgaaac
     1921 tgggctggat gcgctaccgg aacagccgtc aggtcacggg tgttgtgaaa aatgtcactg
     1981 tcagccagtc ctgcggtaag tggtacatca gtattcagac agaaagtgaa gtatcaactc
     2041 cggttcaccc ttcagcatca atggtcgggc tggatgctgg cgtggctaaa ctcgccacgc
     2101 tgtcagatgg cacagtcttt gagcctgtaa acagttttca gaaaaaccag aagaagctgg
     2161 cgagacttca gcgacagtta agccgcaagg tcaaattcag caacaactgg caaaagcaga
     2221 aacgcaaaat acagcgactg cattcctgta tcgcaaatat ccgcagggac taccttcaca
     2281 aagtcacaac ggccgtcagc aaaaaccacg caatgatagt cattgaggat ttgaaggtca
     2341 gcaacatgtc aaagtcagca gcgggtacgg tcagccagcc ggggcgcaat gtccgggcaa
     2401 aatcaggttt aaaccgttcg atactggatc agggctggta tgaaatgcgc cgccagcttg
     2461 cgtacaagca gctctggcgt ggcggtcagg tgctttctgt tccgccagcg tatacaagcc
     2521 agcgttgcgc gtactgtggt catacagcga aagagaaccg cctgtcacaa agtaaattca
     2581 gatgccaggt atgtggatat acagcgaacg ccgatgtaaa tggcgctcgc aacattttag
     2641 cggcggggca cgccgttctt gcctgtggag agatggtgca gtcaggccgc ccgttgaagc
     2701 aggaacccac cgaaatgatt caggcgacag cctgaacgta gcagggatcc acgtccttca
     2761 gggcgtggag gatgtcaata tcggtttctg tcagcgatta aattcaacac cacgtaacaa
     2821 acgcctccgg caattaatcc ccaaaacgca gaaccaatcc cgaccagcgt caatccactt
     2881 gccgttacca gaaatgccac caccgccgcg tctcgctcac gctcattatg cagcgcctga
     2941 tacaaactgc cgccgatggt acttaacagc gccagacctg ccagcatctg gatccaactt
     3001 acgggcaggg cagccatcat cccggtaatg gcactaccaa acagacctgc gagcaaatag
     3061 aaaatgcctg caacggcagc ggccagccaa cgttgatctt tatccggatg cgcttccggg
     3121 ctttggcaaa tagccgcggt gattgccgca ataccgacgg aataaacgcc gaaaggggaa
     3181 aaaaccagtg ccagcaatcc agtaaataca attaatggcg aaacaggagc cgaatatcca
     3241 gctgctttca ttgctgcgat acccggtgcg ttttgcgatg ccatcgtcac cagaaaaagg
     3301 gggagtgcaa cgctcaggct gtgagcaaac gaaaaatcag gggtaatata agtggggaga
     3361 acgggtttaa agacaacatc agttgtgaca acgtcacctt gcgcgatgac gatcacgatc
     3421 ccaataatca tcgcggcaat taccgcatag cgcggcgcaa cggccttggt tgccagccat
     3481 accagcaaca tacttccaca caacgtaaat tgaccgtcca gactggcaaa cgcctgtaaa
     3541 ccaaagcgta ataaaatccc ggcaagcatt gccgccgcaa gcgagtgcgg aataatgcgc
     3601 atcagacgag caaagagtcc cgttatgccg cagaggacta ttagcgcgtt ggtgacaata
     3661 aaaacgccga tggcttcgtt aagtgttagt ccctgcaatc cggtgaccaa caaagccgcg
     3721 ccaggcgttg accatgcggt gagaacaggt acgcgatacc ataatgtcag agtcagcgta
     3781 ctgacgccca ttgccagccc cagcgccgtc atccagccag agatttgtgc agtggtggct
     3841 ccggcgacaa tcgctgcttg ccagattatt gccgctgaac tggcgtagcc aattaatacc
     3901 gccagaaacc ccgccagtag cgtgggtgga ggaatagaaa acagacgcat agtcacctcg
     3961 tgcgttatag cgtacaggca ccgtagcatt tgtccgttat aacgcacaag tgataaactt
     4021 ccgttttgcc ggaggagtcg catggaaaat ctcgctcgct ttttatccac cacacttaaa
     4081 caactacgcc agcagcgcgg ctggagtctt tcgcgactgg cagaagcgac gggcgtttct
     4141 aaagcaatgt tagggcagat tgagcgtaat gagtccagtc cgacggtcgc gacattatgg
     4201 aaaattgcca ccgggttgaa cgtaccattt tcaacattta tttctccgcc gcagtccgct
     4261 acgccttcag tttatgatcc acaacagcag gcgatggtga tcacttcgct gtttccttat
     4321 gatccacaac tctgctttga acacttctcg attcagatgg catctggtgc aataagcgaa
     4381 tcgacgcccc atgagaaggg ggtaatcgaa catgtcgtcg tcattgatgg acaacttgat
     4441 ctgtgcgttg atggggaatg gcagaccctt aattgtgggg aaggcgttcg atttgctgca
     4501 gacgtcacgc atatctatcg taatggtggg gagcaaaccg tacattttca ttccctcatc
     4561 cattacccgc gcagttaagc gggaaaacta tttcgcaacg tcgcgcttgt ggctaaaata
     4621 gccgccattt ttcagctact ggataagaat gtgaccgtat cttctcatcg acttgaactg
     4681 ttaagcccgg cacgcgatgc cgccattgcc cgcgaagcta ttttgcacgg tgccgatgct
     4741 gtttatatcg gcggccctgg ttttggtgcc cgtcataatg ccagtaatag cttgaaagat
     4801 attgccgagc tggtgccgtt tgcccatcgt tatggtgcaa aaattttcgt cacgcttaac
     4861 accattttgc atgatgatga gctggaaccc gcgcaacggc tgattactga cctctaccag
     4921 accggtgtcg atgcgctgat tgttcaggat atggggattc tggaacttga tattccgccg
     4981 attgaactgc acgccagtac gcagtgcgac attcgtacag ttgaaaaagc gaagttcctc
     5041 tctgatgttg gcttcacgca gattgtgctg gcgcgagagc tgaatcttga tcagatccgc
     5101 gcgattcacc aggctacgga cgcgaccatt gaattcttta ttcatggggc actgtgcgtg
     5161 gcctattcgg gtcagtgcta catttctcat gcgcaaacag ggcgtagcgc caaccgtggc
     5221 gattgctcgc aggcgtgccg tttgccatac acattgaaag acgatcaggg gcgggtggtt
     5281 tcctatgaaa aacatctgct gtcgatgaaa gataacgatc agactgccaa cctcggcgcg
     5341 ctgattgatg ctggtgtacg ctccttcaag attgaagggc gttacaaaga tatgagctac
     5401 gtgaagaata tcaccgccca ttatcgccag atgcttgatg ccattattga agaacgtggc
     5461 gatctggcgc gcgcttcatc aggtcgtact gaacatttct ttgttccatc gacggaaaag
     5521 actttccacc gtggtagcac agattatttt gtgaatgccc gtaaaggcga tattggcgcg
     5581 ttcgattcgc cgaaatttat cggcctgccg gtaggcgaag tagtgaaagt ggcgaaagat
     5641 catctcgatg ttgccgttac cgagccactg gcaaatggcg atggcctgaa cgtgttgatt
     5701 aaacgtgaag tcgtcggttt tcgtgccaat acggtcgaga aaaccggaga aaatcagtac
     5761 cgcgtctggc ccaatgaaat gccagcagat ttgcacaaaa ttcgtccaca tcacccacta
     5821 aaccgtaatc ttgatcataa ctggcagcag gcactgacaa aaacctccag cgaacgtcgg
     5881 gtggcggtag acattgaact gggcggctgg caggaacaac tgattctgac cctcaccagt
     5941 gaagagggtg tcagcatcac gcatacgctg gacgggcagt tcgacgaagc caataacgcc
     6001 gaaaaagcaa tgaacaatct gaaggatggt ctggcaaaac tggggcaaac cctctattac
     6061 gcccgcgatg tgcaaattaa tttgccgggg gcgctgtttg taccaaacag tctgttaaac
     6121 cagttccgcc gtgaagctgc tgacatgctg gatgctgcgc gtcttgccag ttaccagcgc
     6181 ggcagccgta aaccggttgc tgatcctgcg ccggtttatc cgcaaacgca tctgagtttc
     6241 ctcgcgaacg tatacaacca gaaagcgcgt gaattttatc atcgctatgg tgtgcagctg
     6301 attgacgcgg cgtatgaagc acatgaagag aagggcgaag tcccggtgat gatcaccaag
     6361 cattgtctgc gctttgcctt taatctgtgc ccgaaacagg cgaaaggcaa tatcaaaagc
     6421 tggaaggcga cgccaatgca actggttaac ggcgatgaag tattaacgct aaagtttgat
     6481 tgccgcccat gcgagatgca cgtcattggc aaaatcaaaa atcacatact gaaaatgccg
     6541 ttaccgggaa gcgtagtggc atccgtaagt ccggatgagc tgctgaaaac attgccgaag
     6601 cgaaaagggt aaaacgccag ttttctggtt actcacaact tattgaatct gcatgatatt
     6661 gcctgccggg taaggcgtta cgccgcatcc ggcatcaaat gactcaacgt tgtcccgctt
     6721 ctggtttgcg cgatttttgc cagtaaaaat gctcgcgcaa accttccgcc gactcttccg
     6781 ccacagcacg caattcatcg ctgtccgctt catgacgcag ctgatgatcc acattcttta
     6841 cccacacaaa ttcatgtcct ttgtgccctg ccatgagttg tcctgaaaac agagcacacg
     6901 ttaataagac aaccgataac gccttcgtaa acatcctgcc acctttttct taccttttgc
     6961 cgctatgatg ccgatcgttt cttgaggtta ttattcagtt ttgcaaatta gcgcaaagaa
     7021 attctggaat cttccttcct gattttgcat tgcattctgc cgttgcggcg atttagtgct
     7081 attttcgagc aaattacaca cggaggtaaa cactaatgtt tactttgttg atatactcag
     7141 cggcagggag gcgatgtgaa acaaagcgag ttcagacgtt ggctcgaatc tcagggcgtc
     7201 gatgtagcga atggcagcaa ccatttgaaa ctcaggtttc atgggaggcg cagtgtcatg
     7261 ccgcgtcacc cctgcgatga gattaaagaa ccattgcgta aagcaatcct gaaacaactc
     7321 ggtttgagtt aatcgccaat taaaaaggtt aatgacatgc gagagacagt cgaaattatg
     7381 cgttatcccg tcactcttac acccgcgccg gaaggcggtt atatggtttc ttttgtggat
     7441 atccctgaag cgttgacgca gggcgaaact gtcgctgaag cgatggaagc ggcaaaagat
     7501 gctttactga ccgcatttga tttttatttt gaagataacg agcttatccc tttaccttcg
     7561 ccattaaata gtcacgatca ctttattgaa gtacctttga gcgtcgcctc taaggtattg
     7621 ctgttaaatg cttttttaca gtcagaaatc actcagcaag agttagccag gcgaattggc
     7681 aaacctaaac aggagattac tcgcctattt aacttgcatc atgcgacaaa aatcgacgcc
     7741 gtccagctcg cggcaaaggc gcttggcaaa gagttatcgc tggtgatggt ttaattacag
     7801 ttaacgaaaa gttgtcattt ttaacaactg atatagactg ccgaatcatc tgcacataat
     7861 tacgattcga taatgaaaaa ataccagcag cttgcagaac aattacgcga gcagattgcg
     7921 tcgggtatct ggcaacccgg cgatcgtttg ccttcgttgc gtgaccaggt ggcgctttca
     7981 ggcatgagct ttatgactgt cagccatgcc tatcagttgc tcgaaagtca gggatatatt
     8041 atcgcacgac cgcagtcggg ttattacgtt gcgccacaga caataaaaat gccgaaagcg
     8101 ccagtcattc cagtcactcg agatgaagca gtcgatatca acacttatat ttttgatatg
     8161 ttgcaggcca gtcgcgatcc gtcggtcgtt ccgtttgcct cggcctttcc cgacccgcga
     8221 cttttccccc tccaacaact aaaccgctcg ctggcgcagg taagcaaaac cgccacagcg
     8281 atgagcgtga ttgaaaactt accgccagga aacgcagaac tgcgtcaggc tattgctcgt
     8341 cgctatgcct tacagggcat caccatttct cctgatgaaa ttgtcattac tgccggggcg
     8401 ttagaggcat taaacctcag tttgcaagcg gtaactgaac cgggcgattg ggtgatagta
     8461 gagaatcctt gtttctacgg tgcgttgcag gcgctggagc ggctacggct gaaggcgtta
     8521 tcggtggcga cggatgttaa agaagggata gatcttcagg cgctggaact ggcgttgcag
     8581 gagtatccgg tgaaagcgtg ctggctgatg actaatagcc agaatccact cggatttacc
     8641 ttaacgccgc aaaaaaaagc acaactggtg gcgttgctca atcagtacaa cgtaacgctg
     8701 attgaagatg acgtttacag cgaactttat tttggacggg aaaaaccgct gcctgcgaaa
     8761 gcgtgggatc gccacgatgg cgttttgcat tgctcttcgt tttcgaaatg tctggtgcct
     8821 ggttttcgta ttggttgggt cgccgccgga aaacatgcac gtaaaattca acgcttgcag
     8881 ttgatgagta cgctttccac cagctcaccg atgcagcttg cgctggtgga ttacctttcc
     8941 acgcgccgat acgacgccca tcttcgtcgc ctgcgtcgtc agcttgcgga acgtaaacaa
     9001 cgtgcctggc aggcactgct gcgttatctg cctgcggaag tgaaaattca tcataatgac
     9061 agcggttatt ttctctggct ggagcttccc gaaccgttag atgccggcga attaagcctg
     9121 gcggcactga cgcatcatat cagtattgcg ccgggtaaaa tgttttctac cggtgaaaac
     9181 tggtcacgtt tttttcgttt taataccgcg tggcagtggg gagaacgtga agaacaggcg
     9241 gtaaaacaat taggtcacct tattaaagaa tacctgaaat agatccccat tcaatttctc
     9301 tcaggttggg taatatgaat ttcgaatagc agtcatattt cctaactcct tgtctatact
     9361 ccagaagata gcttctcaaa cggcataatg cgcggtagct cacaacctga ttaaattttc
     9421 tcaggggcga aggtgtgcct gctagccgcc gtctatggtt aaacaaggag atatttttac
     9481 ggcacggcgg ctgaacaatt aattacgaca ggagtaagac tttatgagca agacatttgc
     9541 ccgcagcagc ctgtgtgcgc tcagcatgac aataatgacc gctcacgccg ccgaaccacc
     9601 taccaattta gataaaccgg aagggcgact ggatattatc gcctggccgg gatacatcga
     9661 acgcggacaa actgataaac aatacgactg ggtaacgcaa ttcgaaaaag agacaggctg
     9721 cgcggtgaat gtgaaaaccg ccgcgacttc cgatgaaatg gtcagtctga tgaccaaagg
     9781 gggttacgat ctggttacgg catccggcga tgcctcgctg cgtttgatta tgggaaaacg
     9841 cgtgcagccg attaataccg ccttgattcc caactggaaa acgctcgatc cgcgcgtggt
     9901 taaaggcgac tggtttaacg ttggcggcaa agtttacggc acaccttacc aatgggggcc
     9961 gaacctgctg atgtacaaca ctaaaacctt cccgacgccg ccggatagct ggcaagtggt
    10021 ttttgttgag cagaatctgc cggacggcaa gagcaataaa ggccgcgtcc aggcttatga
    10081 tggccctatc tacattgcgg acgctgcgtt gttcgttaaa gccactcagc cgcagttggg
    10141 catcagcgat ccgtatcaac tcaccgaaga acagtaccag gcggtgctga aagtgctgcg
    10201 cgatcaacat agtttgatcc atcgctactg gcatgacact accgtgcaaa tgagcgattt
    10261 caaaaacgag ggtgtggttg cttccagtgc ctggccctat caggccaacg ccctgaaagc
    10321 cgaaggccag cctgttgcta ccgttttccc gaaggagggt gttaccggtt gggctgatac
    10381 caccatgctg catagcgaag cgaaacatcc ggtttgcgcc tacaaatgga tgaactggtc
    10441 attaacgcca aaagtgcagg gcgatgtggc ggcctggttt ggctcgttac cggtagtgcc
    10501 ggaagggtgt aaagccagtc cgttattagg cgaaaaaggt tgtgaaacca acggttttaa
    10561 ctatttcgac aaaatcgcct tctggaaaac gcctatagca gaagggggca agtttgttcc
    10621 ctacagtcgc tggacgcagg attacattgc cattatgggc ggtcgctaac ttcgctgggg
    10681 tgctttatga cgtacgcagt ggagtttgac aacgtctcgc ggttgtacgg tgacgtgcgc
    10741 gcagtagatg gcgtcagtat tgcgataaaa gatggtgagt tcttctctat gctggggccg
    10801 tccggctccg gcaaaaccac ctgcctgcgc ctgattgctg gcttcgaaca gctttccggc
    10861 ggggctatct ctatctttgg taaacccgcc agcaatctgc caccgtggga gcgggacgtg
    10921 aatactgtct ttcaggacta cgcgctattt ccgcatatgt cgattcttga caatgtcgcc
    10981 tatgggctga tggtcaaagg cgtgaataaa aagcagcggc acgcaatggc gcaagaggcg
    11041 ctggagaaag tggcgttggg gtttgtacat caacgtaaac cgtcacaact ttctggtggt
    11101 cagcgccagc gggttgctat cgccagagca ttggtgaatg aaccgcgcgt attgctgttg
    11161 gatgaaccgc tcggcgcact ggatctcaaa ttgcgtgagc agatgcagct ggaactgaaa
    11221 aaactgcaac agtctctcgg tatcactttt atcttcgtta cccacgatca gggcgaagcg
    11281 ttatcgatgt ccgatcgtgt ggcggttttc aataatggac gcattgagca ggtcgattcc
    11341 ccgcgcgatc tctatatgcg cccgcgcaag ccgtttgttg ccggattcgt cgggacatcc
    11401 aacgtttttg atggactgat ggcagagaaa ctttgtggca tgacgggaag cttcgccctg
    11461 cgaccggaac atatccgcct caacacccct ggtgaactgc aggccaatgg cacgatccag
    11521 gcggtgcaat atcagggcgc ggcaactcgt tttgaactga aattgaacgg cggtgaaaaa
    11581 ctgcttgtga gtcaggccaa tatgacaggc gaagaactgc ctgccacgct cacgcccgga
    11641 caacaggtga tggtttcctg gtcgcgtgat gtgatggtgc cgctggttga ggagaggtga
    11701 atggcgatga atgtattgca atcaccttca cgtccaggtc tgggtaaggt gtccggtttc
    11761 ttctggcgta atccggggct ggggctgttt ttactgctgc ttggcccgct aatgtggttt
    11821 ggcattgttt atttcggctc gctgctaacg ctgttatggc agggctttta tacctttgac
    11881 gatttcacca tgtcggtaac gccggaactg acgctggcga atattcgtgc gctgtttaat
    11941 ccggcgaatt acgacatcat tctccgcacg ctgactatgg ctgtggcagt cactatcgcc
    12001 agcgccattc tggcttttcc aatggcgtgg tatatggcgc gctataccag cgggaaaatg
    12061 aaagcgtttt tttatattgc ggtaatgttg ccgatgtggg cgagctacat tgttaaagcc
    12121 tatgcctgga cgttattgct ggcaaaagat ggcgtggctc agtggttttt acaacatctt
    12181 gggctggaac cactgctgac tacgttcctt acattacctg cggtgggcgg caatacgctg
    12241 tcaacttccg ggctggggcg ctttctggtg tttctctata tctggctgcc gttcatgatc
    12301 ctgcccgtgc aggcggcgct tgagcgtttg ccgccgtcat tgttgcaggc gtcagctgat
    12361 ctcggcgcac gtccacgaca aacctttcgc tatgtggtgc tgccgctggc gatcccgggt
    12421 attgccgctg gctctatctt taccttctca ctcacactgg gcgattttat cgtcccgcag
    12481 ctggttggcc cgccaggata ttttatcggc aatatggttt attcccagca gggggcgatt
    12541 ggcaatatgc cgatggcggc ggcattcacc ctggtgccga ttgttcttat cgcactgtac
    12601 ctggcgttcg tgaaacgtct gggagcgttc gatgcactct gaacgcgcac cgtttttcct
    12661 caaactggcg gcctggggcg gcgtggtttt cctacatttt cccatcctga ttatcgccgc
    12721 ttatgcgttt aacactgaag atgcggcgtt tagttttcca ccgcagggcc tgacgctgcg
    12781 ctggtttagc gtggcagcac agcgtagtga tattcttgat gccgtgacac tgtcacttaa
    12841 agtggcggcg ctggcgacat taatggcact ggtgttaggg acgctggcag ctgccgcgct
    12901 gtggcgacga gactttttcg gcaaaaacgc catttcgctg ttactgctgc tgcccattgc
    12961 gctgccgggc attgtcactg gtctggcgtt attaaccgcc tttaaaacca tcaatctgga
    13021 gccgggattt ttcaccatcg tggtcggtca tgcgactttt tgtgtagttg tggtgtttaa
    13081 caatgtcatc gcccgttttc gccgcacctc ctggagtctg gttgaggcgt caatggatct
    13141 tggggccaat ggctggcaaa ccttccgcta cgtagtgttg ccgaatctca gttcggcgtt
    13201 actggcagga ggaatgctgg cgtttgcctt gtcgttcgat gaaatcatcg ttacgacctt
    13261 tacggcaggt catgaacgaa cgttaccgtt gtggttgctc aatcagcttg ggcgaccgcg
    13321 tgatgtaccg gtaactaacg tggtggcact gctggttatg ttggtaacaa ccttgccgat
    13381 cctgggggcc tggtggctaa cccgcgaagg cgacaatggt caataaccac tgatacagga
    13441 atatgctatg caacataagt tactgattaa cggagaactg gttagcggcg aaggggaaaa
    13501 acagcctgtc tataatccgg caacggggga cgttttactg gaaattgccg aggcatccgc
    13561 agagcaggtc gatgctgctg tgcgcgcggc agatgcagca tttgccgaat gggggcaaac
    13621 cacgccgaaa gtgcgtgcgg aatgtctgct gaaactggct gatgttatcg aagaaaatgg
    13681 tcaggttttt gccgaactgg agtcccgtaa ttgtggcaaa ccgctgcata gtgcgttcaa
    13741 tgatgaaatc ccggcgattg tcgatgtttt tcgctttttc gcgggtgcgg cgtgctgtct
    13801 gaatggtctg gcggcaggtg aatatcttga aggtcatact tcgatgatcc gtcgcgatcc
    13861 gttgggggtc gtggcttcta tcgcaccgtg gaattatccg ctgatgatgg ccgcgtggaa
    13921 acttgctccg gcgctggcgg cagggaactg cgtagtgctt aaaccatcag aaattacccc
    13981 gctgaccgcg ttgaagttgg cagagctggc gaaagatatc ttcccggcag gcgtgattaa
    14041 catactgttt ggcagaggca aaacggtggg tgatccgctg accggtcatc ccaaagtgcg
    14101 gatggtgtcg ctgacgggct ctatcgccac cggcgagcac atcatcagcc ataccgcgtc
    14161 gtccattaag cgtactcata tggaacttgg tggcaaagcg ccagtgattg tttttgatga
    14221 tgcggatatt gaagcagtgg tcgaaggtgt acgtacattt ggctattaca atgctggaca
    14281 ggattgtact gcggcttgtc ggatctacgc gcaaaaaggc atttacgata cgctggtgga
    14341 aaaactgggt gctgcggtgg caacgttaaa atctggtgcg ccagatgacg agtctacgga
    14401 gcttggacct ttaagctcgc tggcgcatct cgaacgcgtc agcaaggcag tagaagaggc
    14461 gaaagcgaca gggcacatca aagtgatcac tggcggtgaa aagcgcaagg gtaatggcta
    14521 ttactatgcg ccgacgctgc tggctggcgc attacaggac gatgccatcg tgcaaaaaga
    14581 ggtatttggt ccagtagtga gtgttacgct cttcgacaac gaagaacagg tggtgaactg
    14641 ggcgaatgac agccagtacg gacttgcatc ttcggtatgg acgaaagatg tgggcagggc
    14701 gcatcgcgtc agcgcacggc tgcaatatgg ttgtacttgg gtcaataccc atttcatgct
    14761 ggtaagtgaa atgccgcacg gtggacagaa actttctggt tacggcaagg atatgtcact
    14821 ttatgggctg gaggattaca ccgtcgtccg ccacgtcatg gttaaacatt aagttagatg
    14881 ttagtggctg ttataggacg gccactatcg ttttaggttc tgagtttaat ggcggttaag
    14941 tattagaatg attacattaa taactagcgt aaaaattagg ctaaatgttt gagggcgatt
    15001 tttcatcctg t
//
LOCUS       DAZFZC010000109        14765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFZC010000109 DAZFZC010000000
VERSION     DAZFZC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.110"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(17..169)
                     /gene="hokE"
                     /locus_tag="ACHKXP_004073"
     CDS             complement(17..169)
                     /gene="hokE"
                     /locus_tag="ACHKXP_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO7648972.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            138..406
                     /locus_tag="ACHKXP_004074"
                     /pseudo
     CDS             138..406
                     /locus_tag="ACHKXP_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1740
                     /locus_tag="ACHKXP_004075"
     CDS             622..1740
                     /locus_tag="ACHKXP_004075"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO7648973.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPYTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1806..2054
                     /locus_tag="ACHKXP_004076"
     CDS             1806..2054
                     /locus_tag="ACHKXP_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO7648974.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2119..2487
                     /locus_tag="ACHKXP_004077"
     CDS             2119..2487
                     /locus_tag="ACHKXP_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7648975.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2581..3234
                     /gene="nfsB"
                     /locus_tag="ACHKXP_004078"
     CDS             2581..3234
                     /gene="nfsB"
                     /locus_tag="ACHKXP_004078"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO7648976.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3342..4589
                     /gene="ybdG"
                     /locus_tag="ACHKXP_004079"
     CDS             3342..4589
                     /gene="ybdG"
                     /locus_tag="ACHKXP_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO7648977.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4657..6033)
                     /gene="pheP"
                     /locus_tag="ACHKXP_004080"
     CDS             complement(4657..6033)
                     /gene="pheP"
                     /locus_tag="ACHKXP_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO7648978.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWMEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="ACHKXP_004081"
     CDS             complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="ACHKXP_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO7648979.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVSTDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEML
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKTMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9290..10513)
                     /gene="cusB"
                     /locus_tag="ACHKXP_004082"
     CDS             complement(9290..10513)
                     /gene="cusB"
                     /locus_tag="ACHKXP_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO7648980.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10529..10861)
                     /gene="cusF"
                     /locus_tag="ACHKXP_004083"
     CDS             complement(10529..10861)
                     /gene="cusF"
                     /locus_tag="ACHKXP_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO7648981.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11019..12382)
                     /gene="cusC"
                     /locus_tag="ACHKXP_004084"
                     /pseudo
     CDS             complement(11019..12382)
                     /gene="cusC"
                     /locus_tag="ACHKXP_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            12539..13222
                     /gene="cusR"
                     /locus_tag="ACHKXP_004085"
     CDS             12539..13222
                     /gene="cusR"
                     /locus_tag="ACHKXP_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO7648982.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13212..14660
                     /gene="cusS"
                     /locus_tag="ACHKXP_004086"
     CDS             13212..14660
                     /gene="cusS"
                     /locus_tag="ACHKXP_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO7648983.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3563 a         3975 c         3646 g         3581 t
ORIGIN      
        1 gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat
       61 tacgttcctt tacggtgaac tcacacagcg aatctccgac cagaagcgta aatcccagca
      121 ccgttaaaca cagcactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc
      181 ctccatgcga agaagggact accatccaaa ttgttcgggt ttggagtggc agccccaggg
      241 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
      301 aaagcttcag gcacccgccg ttatcttacc cttcctcaac aaaatagctg tttatttata
      361 cagtgagtcc tgtttcggac ttctcactcc tcatttaatc aactgaagct gaacccgttt
      421 ttttctgcat gactcaatcg tcaaaattct tcgccaccag tcagcactgc cataacttgt
      481 cacattgcaa aagtagccaa attgcttcag gcaccatcag ttcaccatca ccaaaaaaaa
      541 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
      601 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
      661 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
      721 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
      781 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
      841 gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag ccacagacca tcatctggaa
      901 atttgcggcg gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgataac
      961 gaacgctatc aacgcacgct ggaaaacttt ggttatctca ttcagcaggc gaccgttttt
     1021 ggtcagcatg tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc
     1081 ttgtcacgat ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata tatgcaggga
     1141 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc tgataatggc
     1201 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tatttcgctg tttgagttac
     1261 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     1321 ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc ggtaaatatg
     1381 gcgggattaa ttcaggccac cgcccactgg ttactgacgg aacgcccgtt caaacatcag
     1441 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     1501 ggcgtcatta ccgatccgta cactggtgat cgtcgaccgc taacggaaga taccttgcga
     1561 ttgctggaaa aaatcgcccc ttccgcacat aaaattggcg catcgagcgc aattgaagca
     1621 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     1681 gatggcggct cgctgattgg gctggtaaaa aagcattgtg agatctgggc cggtgactaa
     1741 gtcgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     1801 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     1861 tcctctcttg cctgctgctt cccgcccccg cattggggct gacgctggca caaaaactgg
     1921 tgagcacgtt ccatctgatg gatcttagtc agctttacac tttattgttt tgtctgtggt
     1981 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     2041 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     2101 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     2161 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     2221 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
     2281 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
     2341 tatcacatga ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg
     2401 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     2461 gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac
     2521 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     2581 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagtaaa
     2641 aaacttaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag cccatccagc
     2701 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     2761 gccaaatccg ctgccggtaa ttatgtgttc aacgaacgta aaatacttga tgcctcgcac
     2821 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     2881 caggaagatg ctgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     2941 cgcaagttct tcgctgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     3001 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     3061 gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg tctgaaagag
     3121 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     3181 gctacgctgc cgaaatcacg tctgccgcaa aacatcacct taaccgaagt gtaattctct
     3241 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     3301 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     3361 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     3421 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     3481 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     3541 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     3601 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     3661 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     3721 agaaattccc ggcagcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     3781 tcggcgcgat tctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     3841 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggtattt aaagatccga
     3901 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     3961 ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     4021 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     4081 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     4141 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     4201 aagcgcattt gttaaagcct tatttaacca gccgccatca ggaaattaat gagtggaatc
     4261 gccagcaagg ttctacggag tcggtattaa acctgcgccg aatgaccaat attggaacct
     4321 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgactt
     4381 taatggtacg ccaactggct ccaggtgata atggtttacc gctcgagatc tatgcgttta
     4441 ccaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     4501 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     4561 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     4621 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     4681 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     4741 catcgtgcac atcagcagca aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa
     4801 cggataaagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ctgcacgaaa
     4861 acgcagatgc gccagacaga tcataatcca gttcaacagc agcgttgcta ccaccagcgc
     4921 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaacacca ccagcgaagt
     4981 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     5041 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     5101 cccgctgtta tacactgaca gcgatgctac cagaatgacg aagttcagcg cagaagctac
     5161 cacgttgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     5221 catccacgga tagagcgcca gtaaaaccac cagtgaaccg atgtaaaaca gcaggatgcg
     5281 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     5341 agtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     5401 cagcccattc cagccggtgg cgaagaaacc accgtagcgc cagaggttgt cgatactggc
     5461 tttctcgccg ccgtgaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     5521 cgccagcact ttaatcagcg caaaccagaa ctcggtttcg ccatataagc gcacgttcac
     5581 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     5641 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     5701 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     5761 ggcaaagtgg gcaaatgaac cggataccgg ctcctcaacc accatttcgc caagctggcg
     5821 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     5881 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccaacgc
     5941 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     6001 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     6061 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     6121 ggggtatcct gcttttattt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     6181 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggcgcg
     6241 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6301 gcgataatca ccgccaccgt catcgttttc gggcgcacgc gcaggaccgc gccgtgatat
     6361 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggcacggcc
     6421 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggcg
     6481 agggcgataa agccagtgcc cgtcgccacg gaaagatgaa agcccatcca ccacagcaac
     6541 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6601 cgacggaacg ccagatacaa cagcacgaag ataatcatca acgtcatcgg caccatgagt
     6661 ttaagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6721 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6781 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6841 agcatcgacg gtccggtaga gactttaatg tcggccacgt ctgccagggt gatttgctgc
     6901 ttcatcggcg tcaggatcgg cagctggcgc agtgcctgcg gactatcgcg ccagctttgc
     6961 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     7021 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     7081 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc
     7141 gcagaagcta cgcctggcac cgttcgcgct acttcttcaa tttgctcagc catcgcgtcg
     7201 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7261 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagccgc
     7321 acggtgttat ccagttcctc aatgattttg tccatcgtca tgcctggccg ccactgctcc
     7381 tgcggcttaa gctggatggt cgtttctaac atctccagcg gagcagaatc ggtggcggtt
     7441 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7501 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7561 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7621 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7681 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cgggttactg
     7741 ctttccggcg gaattttgcc acggatccag tagcccatca ggatcgggat cactacgatc
     7801 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7861 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7921 ctgataaata gcgccggccc cacttcaaca gacgcatcgg tgctcacctg ccagcgcgtt
     7981 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     8041 ttctcgatca tgacgatagc agcatcgacc atcgccccga cggcaatcgc aatgccaccc
     8101 aacgacataa tattggcatt cagtccctgg aagtgcatga caataaaagc aatacacaac
     8161 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgca
     8221 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcg
     8281 cggtcaatga gctggctgcg atcgtatgtt gtaactatct ccacgccttc cggcagacta
     8341 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8401 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8461 cggcgcatct ccgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8521 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgcag atagccgctg
     8581 gcgcgcacca tatattccgc ttccgccagt tcgatcgacg aaccgcccgc ttcctggttt
     8641 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctgg
     8701 ggatcgataa ccacctgata ctctttcacc acaccgccca ccgacgccac ttccgcaacg
     8761 tcagggatgg ttttcagctc atatttgaga aaccagtcct gtaatgagcg taaatcggcc
     8821 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8881 gcatctggcc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8941 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     9001 acataagagt cgccaaactg tgagaaaccg cgcacagtct tcgcgccagg caccgacaac
     9061 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgtggtgc ctgaccggga
     9121 tagctggttt taataataac ctgcacatcg gagagatccg gcagcgcatc cactggcgta
     9181 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9241 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9301 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9361 tcaggaacag gccgctggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9421 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9481 caacggtaat cacccgctgt tcgctgccgg tatcaatcag cgcttgtgac ggaatgagca
     9541 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9601 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9661 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9721 taaactgcga ggcatctttc accagccagg cgatagactc cgggatcgca gcagtgaccc
     9781 acaccgggtc cataccctga attttcgcta ccacgttatc tttggcgata ttcattcccg
     9841 cgcgcagatc aaacgcggtg atcacgccat caatgggcgc tttgagcgta aagcgagtct
     9901 ggattttttg cgtggcgatc aggcggcgaa tatccgcctc cggcattccc gccagtcgca
     9961 gccgctcaag aatgccttca gtctgggtcg ccgtaccgcc ggtttcgcgc agcagtaaat
    10021 actcactctg cgcttccacc cagtcaggaa tggtcaggtc gagaagcggt gtgccctttt
    10081 gtactttatc gcccacggta agcggataca ccttgtcgat aaacccggca gcgcgagcct
    10141 gcacaatggc atactgatac tcgttgtaac tgacattcgc cgggaaactc tgggcaaaag
    10201 tcagcggtcc gcgcgtgacg gtagccgttt tcacccccag attctgcgtc tgagtcgggt
    10261 caatgcgcac accagacgca gaactctctt catcggcata tttcggcacc agatccatat
    10321 ccataaacgg cgatttacct ggtttatcga accgcgtatt gggatacatt gggtcgtacc
    10381 agaataagat tttacgttct gcggtcgacg ttttttctgc aggcggttcc gcctttgcaa
    10441 cccaggtaaa acctgccgca gaaataatac cgcccgcgat catgctgccg ataataagcg
    10501 cgattttttt catctcatta aacctgggtt actggctgac tttaatatcc tgtaataaag
    10561 aaaggttgcc ctgctggaca aaattaaacg ccactttgtc gccggtttta atttcactca
    10621 ttttcgtctg cggggtgatg gtaaagcgca tggtcatctc cggccagttc acggcagcaa
    10681 tcggatcgtg atggatggtg atttttttgc tttccagatc aatacccttt accacgccag
    10741 tggcgctaat aacctgtggt tgtgcttcgc tcatggtttc atgatgatgt tcgttagcct
    10801 gggcattaaa gccaataacg gtaaacagac tgaacattgc gacttgcagt gcttttttca
    10861 tttattctct cctggagtta aaagtcatac tctaaataat tcgagttgca ggaaggcgac
    10921 aagcgagtga atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg
    10981 cagcacatgc aacttgaagt atgacgagta tattgctgtc aaccgccacc aagtgcggta
    11041 tacaaagaaa tttcgttaac ctgacgggca taattcagat caagtaaagt ttgtcgggtt
    11101 gcaaataaag aacgctcggc atccagcact tccagataac ttactgcgcc gtgctgatat
    11161 aatgcccgcg cccgttgcaa agtaatttgc agcgatgcca gataacgctg ctgggcgcta
    11221 atttgatcgt tcaggctttg acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc
    11281 tggattttct gttcataatt caccaccgac tgctgctggc gaatttcggc gatatccaga
    11341 ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttgggtat aaaattccac
    11401 atcccgctgc tggcgttaaa taatgacgat agatcgctac tggcggtcga tattccgctg
    11461 gtcaggctta tagacggaaa aaatgccgcg cgtgcagcgc caatattggc attagccgcc
    11521 attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg cgacgacaat
    11581 cccgccggta atttaactct ttgcaggctg tcgctgttta ctgtctgcgc ttgccgtagc
    11641 ttcccagcaa cagttgcaat gcattattcg cctgcgccag ttccccctga cgtttagcga
    11701 tgtcgctgcg ggtactttct atcaccccgc gagcctgttc cagcgccaga acattactgc
    11761 tacctgtcaa cagttgtttt tcgacaaacg catatgactg ctgataatta cgcagcgttt
    11821 cttcggctat ttgcaattgc gcatacgcca gttgctgatt gaaatagctt tgcgcgacat
    11881 tagaaaccag cagaatatgc accgcgcgct gagcttcctc agtggctaaa taattttgtc
    11941 gctcggcttc gctcatgttc tttaagcgac cgaaaaaatc gaggtcaaag ctggcgttaa
    12001 ggccggtcga aaactcccgt gtcgtggcgg tattcccttt aagattaccg ctccagctgc
    12061 cgctgccctc gccattgagc tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc
    12121 gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg attattcacc agcgcctcgc
    12181 taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg ccagcccgcg ttctgatagt
    12241 tatctgctgc gttaaccagg ccgttctggc tgagtgagaa ctgctgcggc acgggcattg
    12301 ccggacgctg ataatccggt gccagtgaac aaccggttag cgcaagggcc acacaaaatg
    12361 gcagaagttt acaaggagac ataggctcat aatttctggt gattttatgc cgccaacttt
    12421 actcgccagg ctctgatttt ccggtgacag aaaaatgaca aaattgtcat tttgccaata
    12481 agcgattgcc atctgatccc gctactctag aattgcccgg gcaacatgcg gaggaaatat
    12541 gaaactgttg attgtcgaag atgaaaagaa aaccggagaa tacttgacca aagggttaac
    12601 cgaagccggt tttgtggtcg atttggccga caacgggctg aatggctacc atctggcgat
    12661 gaccggtgat tatgatctga taatcctcga tattatgctg ccggacgtga acggctggga
    12721 tatcgtgcgc atgttacgct ccgccaataa agggatgccg attctgttgc ttaccgcgct
    12781 tggcaccatt gaacatcgcg tcaaggggct ggagttgggg gcagatgact acctggtgaa
    12841 gccattcgct tttgctgaac tgctggcgcg ggtgcgcaca ttactgcggc gcggggcggc
    12901 ggtgattatc gaaagtcagt ttcaggttgc cgatttgatg gtcgatctcg tcagccgcaa
    12961 agtcacccgc agcggcacgc gcatcacttt gaccagtaaa gagtttactc tgctggagtt
    13021 cttccttcgc catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc aggtatggga
    13081 catgaatttt gacagcgata ccaatgctat tgatgtggcg gtgaagcggc tgcgcggcaa
    13141 aatcgacaac gactttgagc cgaagctaat tcagaccgtg cgcggcgtgg gttacatgct
    13201 tgaggtgccg gatggtcagt aagccatttc agcgcccgtt ttcgctggca acccgcctga
    13261 ccttttttat cagcctggcc accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc
    13321 actcagtaaa agttcatttt gccgagcagg atattaatga tttaaaagag attagcgcca
    13381 cccttgaacg ggtactaaat caccctgacg aaacgcaagc ccgacgctta atgacgctgg
    13441 aagatatcgt cagtggttat tccaatgtgt tgatttccct ggcagatagt cagggtaaaa
    13501 cggtgtatca ctcccccggt gcgccggata tccgcgagtt tacgcgtgac gccatacccg
    13561 ataaagacgc tcagggtggc gaggtgtatc tcctttccgg cccgacgatg atgatgccag
    13621 gccacggtca cgggcatatg gaacacagca actggcggat gattaacttg ccggttggcc
    13681 cgttggtgga cggcaaaccg atttatacgc tctacatcgc gctttcgatc gattttcatc
    13741 ttcattacat aaatgatttg atgaataaac ttattatgac cgcatcggta atcagcatcc
    13801 tgatcgtctt tatcgtactg ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca
    13861 gccgtcaaat ccagaatatt acctcgaaag atctcgacgt tcgcctcgac ccgcagaccg
    13921 tgccgattga gctggaacag ctggtactgt cgttcaacca tatgatcgag cgtattgagg
    13981 atgtctttac ccgccagtcc aatttctcag cggatatcgc tcacgaaatt cgcacgccga
    14041 ttaccaatct catcacgcaa acggaaatcg ccctcagcca gtctcgcagc cagaaggagc
    14101 tggaagatgt gctctactct aatctcgaag agctgacgcg aatggcgaaa atggtcagcg
    14161 atatgctgtt tctcgctcag gccgataaca accagctaat ccccgaaaag aaaatgctca
    14221 acctggcgga tgaagtcggc aaagtgttcg attttttcga ggcgttagcg gaagatcgcg
    14281 gcgtggagtt gcgatttgtt ggcgacaaat gtcaggttgc gggcgatccg ctgatgctgc
    14341 gtcgggcgtt aagcaacctg ctttctaatg ccctgcgtta tacgccaccc agtgaggcta
    14401 ttgtagtgcg ctgccagacg gtcaatcatc aggtgcaagt ttccgtcgaa aaccccggta
    14461 cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg tttctatcgc gttgaccctt
    14521 cccgccagcg aaaaggtgaa ggtagcggta ttgggctggc gatagtgaaa tcgattgttg
    14581 tcgcgcataa aggcacggtt gcggtaacgt cagatgcgcg ggggacaagg tttgtgatta
    14641 tgctgccgga gagagagtga ttattaagtt caaattcagg gcgtggtgca tcacctcctg
    14701 gtattgttga tcaacaaatt agagaaaaac ttattaaaca tatgaaatat cgaaggcttt
    14761 tattt
//
LOCUS       DAZFZC010000110        14674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFZC010000110 DAZFZC010000000
VERSION     DAZFZC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.111"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..905
                     /gene="gspC"
                     /locus_tag="ACHKXP_004087"
     CDS             90..905
                     /gene="gspC"
                     /locus_tag="ACHKXP_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7648984.1"
                     /translation="MPTLRFPFHLANHNKDAAINILIIFISIGSIIFNVNYFHTTIVK
                     NGQIINQPTNAFQSDFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVGELTGMNGLRHERTQ"
     gene            889..2841
                     /gene="gspD"
                     /locus_tag="ACHKXP_004088"
     CDS             889..2841
                     /gene="gspD"
                     /locus_tag="ACHKXP_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO7648985.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2851..4332
                     /gene="gspE"
                     /locus_tag="ACHKXP_004089"
     CDS             2851..4332
                     /gene="gspE"
                     /locus_tag="ACHKXP_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7648986.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPDRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHKALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            4329..5525
                     /gene="gspF"
                     /locus_tag="ACHKXP_004090"
     CDS             4329..5525
                     /gene="gspF"
                     /locus_tag="ACHKXP_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7648987.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5535..5972
                     /gene="gspG"
                     /locus_tag="ACHKXP_004091"
     CDS             5535..5972
                     /gene="gspG"
                     /locus_tag="ACHKXP_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7648988.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5980..6489
                     /gene="gspH"
                     /locus_tag="ACHKXP_004092"
     CDS             5980..6489
                     /gene="gspH"
                     /locus_tag="ACHKXP_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7648989.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6486..6863
                     /gene="gspI"
                     /locus_tag="ACHKXP_004093"
     CDS             6486..6863
                     /gene="gspI"
                     /locus_tag="ACHKXP_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7648990.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6856..7443
                     /gene="gspJ"
                     /locus_tag="ACHKXP_004094"
     CDS             6856..7443
                     /gene="gspJ"
                     /locus_tag="ACHKXP_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7648991.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7436..8419
                     /gene="gspK"
                     /locus_tag="ACHKXP_004095"
     CDS             7436..8419
                     /gene="gspK"
                     /locus_tag="ACHKXP_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7648992.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8434..9597
                     /gene="gspL"
                     /locus_tag="ACHKXP_004096"
     CDS             8434..9597
                     /gene="gspL"
                     /locus_tag="ACHKXP_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7648993.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLLLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9594..10055
                     /gene="gspM"
                     /locus_tag="ACHKXP_004097"
     CDS             9594..10055
                     /gene="gspM"
                     /locus_tag="ACHKXP_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO7648994.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            10055..10732
                     /gene="gspO"
                     /locus_tag="ACHKXP_004098"
     CDS             10055..10732
                     /gene="gspO"
                     /locus_tag="ACHKXP_004098"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO7648995.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10761..11237)
                     /gene="bfr"
                     /locus_tag="ACHKXP_004099"
     CDS             complement(10761..11237)
                     /gene="bfr"
                     /locus_tag="ACHKXP_004099"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO7648996.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11309..11503)
                     /gene="bfd"
                     /locus_tag="ACHKXP_004100"
     CDS             complement(11309..11503)
                     /gene="bfd"
                     /locus_tag="ACHKXP_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO7648997.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11672..14365)
                     /gene="chiA"
                     /locus_tag="ACHKXP_004101"
     CDS             complement(11672..14365)
                     /gene="chiA"
                     /locus_tag="ACHKXP_004101"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO7648998.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKSNDASNPWRYVRAATATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNSEPSTPADSVND
                     YSLQAWSGQEGSEIYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLERTATAAE
                     LSQYGNPTTCEIDNGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGD
                     NPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPW
                     KPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRV
                     FVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKD
                     ANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQH
                     YYDIVDNLNLKVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPIL
                     PTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIAN
                     LHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVG
                     IWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3825 a         3745 c         3458 g         3646 t
ORIGIN      
        1 ttttgcttga ttcatgcaag cggcattaat actatttata ctaacgtcaa tatacaaccc
       61 acccaatcta ttttattaga acatacatcg tgcccacact acgtttcccc ttccatctgg
      121 cgaatcataa taaagatgct gccatcaata tattgattat atttataagc atagggtcga
      181 ttatttttaa tgtgaattat ttccatacaa ctatagttaa aaatggacag atcattaatc
      241 aaccaacaaa cgcatttcag tctgatttca gccttgccgc tttatggcgt aacgaaaatc
      301 atgctggggt aaaagatgca aatcccgtgg cagttaatca ggaaacacct aagctctcta
      361 tagcgcttaa tggcattgta ctcacatcga atgatgagac ttcttttgtc ttaattaatg
      421 aggggagcga acaaaagcga tactccctca acgaagccct tgagtcagcg ccgggaacgt
      481 ttattagaaa aataaataaa accagcgttg tgtttgaaac tcacgggcat tacgaaaaag
      541 taactctcca tccagggcta cctgacatca tcaagcagcc tgattcagaa agtcaaaatg
      601 tactcgctga ttatattatc gccacgccta tacgtgatgg agaacagata tacgggcttc
      661 gattgaaccc tcgtaagggc ctcaatgcat ttaccaccag cttgctacaa ccaggggata
      721 tcgccctcag gatcaataat ctttcgctga cccaccctga tgaggtttca caggcattaa
      781 gtttactgct aactcaacaa agtgctcagt ttacaattcg tcgcaacggc gtaccccgct
      841 tgataaatgt ttccgtcggg gaacttacag gaatgaatgg actgcgtcat gaaaggactc
      901 aataaaatca cctgctgctt gctggcagca ctactcatgc cttgtgcagg acacgctgag
      961 aacgaacaat acggcgcgaa cttcaataac gccgatatcc gccagttcgt ggaaatagtg
     1021 ggtcagcatc ttggcaaaac gatcctgatc gacccttcgg tacagggaac catttccgta
     1081 cgcagtaatg atacgtttag ccaacaggag tactaccagt tctttttaag tattcttgat
     1141 ctttacggtt attccgtgat cacgctggac aatggttttc tgaaagtggt tcgctcagct
     1201 aatgtaaaaa catcgccagg gatgattgct gacagttctc gtccaggcgt aggtgatgag
     1261 ttggtcaccc gaattgtacc gcttgagaac gttcctgctc gtgacctggc ccccctgctc
     1321 cgccagatga tggatgcggg tagcgtcggt aatgttgtgc attatgaacc ctccaacgtt
     1381 cttattctga ccggtcgtgc ctccaccatt aataaactga ttgaagtcat aaagcgcgtt
     1441 gatgtcatcg gcacagagaa gcagcaaatt attcatctgg aatatgcgtc agcggaagat
     1501 ctcgccgaga ttcttaatca attaatcagc gaaagccacg gtaaaagcca gatgccagcc
     1561 ctcctctccg cgaagattgt ggcggataag cgaaccaact ctcttatcat cagtggaccg
     1621 gaaaaagcac gccagcgcat cacttcatta ctgaaaagcc ttgatgtcga agagagcgag
     1681 gaaggaaata cccgggttta ttacctgaaa tatgctaaag ccacgaatct ggtggaagtg
     1741 ctaaccggtg tttccgaaaa gctgaaagat gaaaaaggga atgcgcgtaa gccctcctct
     1801 tctggcgcga tggataacgt cgccattacc gccgatgaac agactaactc tctggttatt
     1861 accgctgacc agtccgtcca ggaaaaactc gccacggtaa ttgcgcgtct ggacattcgc
     1921 cgtgcacagg tgctggttga ggcaatcatc gttgaagttc aggatggaaa tggactaaac
     1981 ctcggcgtgc aatgggcgaa taaaaacgtt ggcgcacagc aatttaccaa taccggatta
     2041 ccgattttta acgctgcgca aggtgtggct gattataaaa agaatggtgg gatcaccagc
     2101 gcgaatcctg cctgggatat gtttagcgcc tacaatggca tggccgcagg cttcttcaat
     2161 ggcgactggg gagtactgct taccgcgctg gccagtaaca ataaaaatga catcctcgcc
     2221 accccaagca tcgtaacgct ggataataaa ctcgcgtcct tcaacgtggg gcaggatgtg
     2281 ccggtgctat ccgggtcaca gaccacttca ggggataacg tctttaatac cgtcgaacgc
     2341 aaaacggtgg ggacaaaact caaagttact ccgcaggtca atgaaggcga cgcggtgttg
     2401 ctcgaaatag agcaggaagt ctccagcgtt gactcttcct ctaactcgac gctcggcccg
     2461 acgtttaata cccgtactat tcaaaacgcc gtgctggtca aaaccggtga aacggtggtc
     2521 ctgggcggat tgctggatga tttttctaaa gagcaagtgt caaaggttcc tctgcttggc
     2581 gatattcctt tagtggggca actcttccgc tatacctcca ccgagcgcgc taaacgcaac
     2641 ctgatggtat ttatccgtcc gacgattatc cgtgacgatg atgtttatcg ctcactgtca
     2701 aaagagaaat acacccgtta ccgtcaggag caacaacagc ggatcgacgg gaaatcaaaa
     2761 gcgctggttg gctcggaaga tttgccggtg ctggatgaaa acacgttcaa cagtcacgcc
     2821 cctgcgccat cgtcacggtg aggcattcat atgagaattc actcaccgta ccccgccagt
     2881 tgggcgctgg cacaacgaat tggttatctc tattcagagg gcgagattat ttatctcgcc
     2941 gatacgccat tcgagcggtt actcgatatt caacgtcagg ttggccagtg ccagaccatg
     3001 accagcttgt cacaggctga ttttgaagct cggctggaag cggtattcca tcagaatacc
     3061 ggtgagtcgc aacagattgc gcaggatatc gatcaatccg tcgatcttct ctcgctttcg
     3121 gaagagatgc ccgcaaatga agatctcctg aatgaagatt cagcggcacc ggttatccgc
     3181 ttgatcaatg cgattttgag tgaggccatc aaagaaaccg cctctgatat ccacattgaa
     3241 acctatgaaa aaacaatgtc gatccgtttt cgcatcgacg gcgttttgcg gacaatttta
     3301 cagccaaaca aaaaactggc ggcactgctt atctcccgaa ttaaggtcat ggctcgtctt
     3361 gatatcgccg aaaaacgtat tccacaggat ggaagaatta gtttgcgtat cgggcgacgt
     3421 aacatagatg tccgcgtatc cacactgccg tccatctatg gtgaacgcgc cgtactccgc
     3481 ctgctggata aaaacagcct ccagctttca ttgaacaacc tggggatgac ggcagcggat
     3541 aagcaggatt tagaaaatct cattcagctt ccgcacggta ttatcctggt gacagggccg
     3601 acaggctccg gtaaaagcac cacgctctac gccatccttt cggcgctgaa tactcccgac
     3661 cgcaatattc tgacggtaga agatcccgtg gaatatgagc tggaaggcat tgggcaaacg
     3721 caggtgaata cccgtgtgga tatgtctttc gctcgcggcc tgcgcgccat acttcgccag
     3781 gacccggatg tcgtcatggt gggggaaatt cgtgatacag aaaccgcgca gattgcggtt
     3841 caggcctcgc tcaccggcca tctggtactc tcaacactcc acactaacag tgcatcaggc
     3901 gcagtgaccc ggctccgcga catgggcgtc gaatcattcc tgctttcgtc ttccctggca
     3961 gggattatcg cgcaacgtct ggttcgtcgc ctgtgtccgc aatgccgaca attcacgccc
     4021 gtatcacccc aacaagcgca gatgtttaaa tatcatcagc tcgcggtgac aacaattggc
     4081 actcccgtag gctgccctca ttgccatcaa tccggctatc aggggcgcat ggcgatccac
     4141 gaaatgatgg tggtgacgcc ggaattacgg gccgctattc atgaaaatgt ggatgaacaa
     4201 gcactggagc gactagtccg gcaacaacac aaggccttaa tcaaaaatgg cctgcaaaaa
     4261 gtgataagcg gtgacacctc ctgggatgag gttatgcgcg tcgccagtgc cacgctggag
     4321 agcgaagcat gaattatcgc tatcgcgcca tgacccagga tggtcaaaaa ttgcaaggga
     4381 tcattgatgc taacgatgaa cgtcaggcac gactgcggct gcgtgaagaa gggcttttcc
     4441 tgctggatat tcgcccccaa aaaagttcgg gagtaaaaac acgtcgcccg aggatcagcc
     4501 atagtgaact gacgcttttc acccggcagt tggcaacctt aagcgcagcg gcattacccc
     4561 tggaagagag ccttgccgta atcggtcaac aaagcagtaa taaacgactg ggtgacgtgt
     4621 taaatcaggt acgcagcgcc atccttgaag ggcatcccct ttccgatgca ttacagcatt
     4681 ttcccacgct tttcgattcg ctctatcgta ccctggtaaa agcgggcgaa aagagcgggc
     4741 tgctggcccc ggtgttggaa aagctggctg attacaatga aaaccggcag aaaatccgca
     4801 gcaagctcat tcagtcactg atctacccct gtatgctcac tacggtggcg attggggtcg
     4861 tgattattct cctcactgct gtcgtgccca aaattaccga acagttcgtg catatgaagc
     4921 agcaactgcc gctgagtaca cgcattcttt taggtctgag cgacacgttg caacgtaccg
     4981 gcccgacatt attagcgaca gtgtttattg tcgctgtagg tttctggctc tggttaaaac
     5041 gcggcaataa ccgccaccgt tttcatgcca tgttgctgcg cgttgcgctc atcggcccgc
     5101 tgatttgcgc cattaacagc gcacgctatc tccgcacttt aagtattttg caatccagcg
     5161 gcgtccctct gctggatggg atgaatttgt ccaccgaaag cctcaacaac ctcgaaattc
     5221 gccagcgtct ggcaaatgcg gcagagaacg ttcgccaggg taacagcatt catctttcgc
     5281 tggaacaaac cgcaattttc ccgccgatga tgctctacat ggtggcctct ggcgaaaaaa
     5341 gcgggcagct cggcacatta atggtcagag ccgcagataa ccaggagaca ctccaacaaa
     5401 atcggatcgc cttaacgctc tccatcttcg agccagcact cattattacg atggcactga
     5461 tcgtcctgtt tattgtcgtg tcggtactcc aacctcttct tcaacttaac tcaatgatta
     5521 attaaggaaa gcatatgcgc gcaacggata agcaacgcgg ttttacatta ctggaaatta
     5581 tggtggtgat cgtcattatc ggcgtacttg ccagcctggt ggttcctaac ctaatgggca
     5641 ataaagaaaa ggcggataag caaaaagccg tcagcgatat tgtggcgctg gaaaacgccc
     5701 ttgatatgta caaactcgac aatcatcact atccaaccac aaatcagggg cttgaatcct
     5761 tagtagaagc gccgacactg ccaccgctgg ccgcaaacta taacaaggaa ggttatatca
     5821 agcgtctgcc tgccgatccc tggggcaatg attatgtcct cgttaatcct ggtgaacatg
     5881 gtgcatacga tctgctttca gcagggcccg atggtgaaat gggaaccgag gacgacatca
     5941 ccaactgggg tttgagcaag aagaaaaagt aagtcagcga tgaatcagca acgcgggttt
     6001 accctgctgg agatgatgct ggtgctggcg ctggtcgcta tcacggcaag cgtggtgctc
     6061 ttcacatatg gccgggagga tgtcgccaac actcgtgcgc gcgaaacagc ggcgcggttt
     6121 accgctgcac ttgaattagc catcgaccgc gccacgctca gtggtcagcc tgtcggtatc
     6181 catttctctg attcagcatg gcgcattatg gtaccgggca aaacaccatc agcctggcgc
     6241 tgggttccat tacaagaaga tgccgcagac gagagccaaa atgactggga tgaagaactc
     6301 tcaattcatc ttcagccatt taaacctgat gacagcaacc agccacaggt tgtgattctg
     6361 gccgacggac aaatcacgcc cttctctttg ttgatggcta acgccgggac cggtgagccg
     6421 ctcctgacgt tagtctgttc aggctcatgg cccctggatc aaacgctcgc cagagatacc
     6481 cgcccatgaa caaacaatca gggatgacac tgcttgaagt cttactggcg atgagtattt
     6541 tcaccgctgt tgctctgact ttaatgagca gtatgcaagg gcaacgaaat gccatcgagc
     6601 gaatgcgtaa cgagacgctg gcattgtgga tagcggataa ccagcttcag tcacaagatt
     6661 cattcggcga ggaaaacacg tccagttctg gcaaagagtt aataaatggc gaagagtgga
     6721 actggcgcag cgatatccac tcaagcaaag acggcacgct tctggaacgc accataacgg
     6781 tgacattacc cagcggtcag acaacctcgc ttacgcgtta tcaaagcatc gataataaat
     6841 ccgggcaggc acaagatgat taatcgccag cagggtttca cattgctgga ggtcatggcg
     6901 gcgctggcga ttttctcaat gcttagcgtg ctggcgttta tgattttttc gcaagcttcc
     6961 gagttgcatc aacgctcaca gaaagaaatc cagcagttca atcagctaca gcgcaccatc
     7021 accattcttg ataacgatct cctgcaactg gtcgcccgga ggaatcggag tacggacaaa
     7081 atcatggtgt tgggtgaaga agctattttt accacgcaaa gtcgcgatcc gctggctcct
     7141 ctcagtgaag cacaaacctt actcactgtt cactggtatc tgcgaaatca tacgctctac
     7201 cgtgctgttc gtacttctgt ggatggcagg aaggatcaac ccgcccaggc gatgctggaa
     7261 catgtcgaga gctttcttct ggaaagtaac agcggggaaa gccaggaact tcctctctca
     7321 gtcacgttac atctgcagac tcagcaatat ggggggttac agcgccgttt tgcattacct
     7381 gagcagttag cgagagaaga gtcgccagct cagacgcagg ccgggaataa taaccatgaa
     7441 taatgaacaa cgcggcgtcg cgctgctcat tgtactcatg ttactggcgc tcatggctgc
     7501 tctggcagca gacatgacgc tcagttttca cagccagctt cagcgaaccc gccaggtaaa
     7561 ccatcatctg caacggcaat atgatattga actggcggag aaacttgcgc ttgccagcct
     7621 cacccaggac gttaaagata acgacagaca gaccacgttg cagcagtact gggcacagcc
     7681 acaacagttg cagctggaag atggcaatac agtgaagtgg cagttacgcg atgcccagca
     7741 ctgttttaat ctcaatgcgt tagcaaaaat ctctgacgac ccgctggctt cccctgattt
     7801 ccccgcccag gtattcagtg cgctgctgat taacgccggt atcgatcgcg gaaacaccga
     7861 tgagatcgtg caatcaatcg ctgactatat tgatgtcgat gactcaccac gctttcacgg
     7921 cgcagaagat agcttttacc aaagccagac gccgccccgg catagtgcca atcagatgct
     7981 ttttctgact ggcgaattac gtcagataaa aggcataacc gaaaacatct atcagcggct
     8041 tatcccgtat gtctgcgttc tcccgaccac ggaacttagc atcaatctta atatgctaac
     8101 ggagaacgat attccgctct tcagagcgtt attcctcaat aacatcacgg atgctgacgc
     8161 tcgggtctta ttgcaaaaaa ggccgaggga aggctggcta accacggatg catttcttta
     8221 ctgggcgcag caggatttct ccggtgtgaa gccactggtt gctcaggtga aaaggcatct
     8281 ctttccctat agccgctatt ttacgctgag tacggaaagt atcagcgacg aacaatccca
     8341 gggatggcaa agccatattt ttttcaaccg taaacagcag agcgcccaaa tctatcggcg
     8401 tacgctgcaa ctctactaat cataaggatc gtgatgcctg aatcgttgat ggtcatacgt
     8461 tcttcctcga cgctgcgtaa gcattgggaa tggatgacgt tctccgcaga tagcgtatct
     8521 tcggtacata cgctcacgga cgatcttctg ctggagtcgc tggccgatca gcccggtgcc
     8581 ggaaatgttc atcttttgat cccaccagag ggccttttat atcgttccct cacgctgccc
     8641 aacgccaaat acaaactcac cgcacaaaca ttacaatggc tggcagagga gacgttaccc
     8701 gacaacaccc aggactggca ctggacggtt gtcgataaac aaaacgaaag tgtcgaggtg
     8761 ataggcattc agtcggagaa actcagtcgc tatcttgagc gtttacatac tgccggactc
     8821 aacgtgacgc gggttttgcc agatggctgc tatcttccct gggaagtaga tagctggact
     8881 ctggttaacc agcaaacaag ctggctcatt cgcagtgccg cacatgcctt caacgaactg
     8941 gatgaacact ggttacagca tcttgctgcc cagtttccgc ctgagaatat gctttgttat
     9001 ggtgtagtac ctcacggcgt tgcagcggcc aatccactta tccagcatcc tgaaatacca
     9061 tcactgagtt tgtattctgc cgatattgca ttccagcgct atgacatgct gcatggtatt
     9121 tttcgtaaac agaaaactgt aagcaaatcg ggaaaatggc tcgcccgcct ggcagtaagc
     9181 tgtctcgttc tcgccattct ttcttttgtt ggcagccgga gcatcgcgct ctggcatacg
     9241 cttaagattg aagatcaact tcagcaacaa cagcaagaaa cctggcaacg ttatttcccg
     9301 cagatcaaac gcacccacaa ttttcgtttt tactttaagc agcaactcgc tcagcaatat
     9361 cctgaagctg tgccgttgct ctatcattta caaacacttc tgctggaaca ccctgaactg
     9421 cagttaatgg aggcgaacta tagccagaag caaaagtcgc tgaccttaaa aatgagtgct
     9481 aagagtgaag ctaatatcga tcgtttctgt gagttaacac agtcctggct tccgatggaa
     9541 aaaacagaaa aagatccggt cagtggtgta tggacagtaa ggaactcagg gaaatgatta
     9601 aatcatggtg ggcagaaaaa tccacatcgg aaaaacagat cgttgcggca ctggcggttc
     9661 tctcgctcgg cgttttttgt tggcttgggg tgataaaacc gatagatacc tacattgcag
     9721 agcatcagtc ccacgcgcaa aaaattaaga aggacatcaa atggatgcag gatcaagcga
     9781 gcacccatgg tttactcggt catcctgcgc ttacgcagcc catcaaaaat atccttctcg
     9841 aggaagcaaa acgtgagaat ttggctatca cgctggaaaa cggcccggac aatactctga
     9901 caattcatcc tgtcacagct cccctcgaaa acgtctcccg ctggctcact acggcacagg
     9961 taacatacgg tattgttatt gaagaccttc agtttactct tgccggaaac gaagagatca
    10021 ccctcaggca tttgtccttc agggagcaac aataatgaca atgctactgc cgctcttcat
    10081 tctggttggt tttattgcag gttattttgt taatgccatc gcctatcacc tctcgccgtt
    10141 ggaggataaa acggcgttaa cgtttcgcca ggtattggtt catttcaggc aaaaaaaata
    10201 tgcctggcat gatacagtgc ccctgatact ttgtgttgct gctgctatcg cctgcgccct
    10261 ggcacctttc acgcccatcg tgactggcgc actctttctc tatttctgtt tcgtactcac
    10321 gctcagtgtt attgattttc gcactcagct cctgcccgac aaactcacct taccgctgct
    10381 ctggcttggc ttggtattta atgcgcagta tggattaatt gatttacatg atgcggttta
    10441 cggcgcggta gcgggctatg gggtgctgtg gtgtgtttac tggggcgtct ggttagtttg
    10501 tcacaaagag ggattgggct acggtgattt caagctactg gccgctgcag gcgcatggtg
    10561 tggctggcaa acgttgccaa tgatactgct gattgcctcg ctgggtggca ttggttacgc
    10621 catcgtttca caacttctgc aacgccggac tataaccact atcgcattcg gcccgtggct
    10681 tgcgctcggc agcatgataa acctggggta tctggcctgg atctcttatt aaccgggagg
    10741 gttctccctc ccgacacggc tcaaccttct tcgcggatct gtgcttgcag ataattttgc
    10801 aggcccatct tctgaatcag atcaagttcc gtttccagcc agtcgatatg gccttcttca
    10861 tcacgcaaaa tttctatcat catatcgcgg ctgacgtaat catgaacgct atcggcataa
    10921 ccaattgcct cacgcaaatt cttcgcgcca tccagctcaa gtgccagatc agaacgcagc
    10981 atttcctcaa catcttcacc aatgttcagt ttgcccaggt cctgtaagtt tggaagacct
    11041 tccagaaaaa gaatgcgctc aatataacga tcggcgtgtt tcatctcatc aatggattca
    11101 tgatactcca catcattgag acgtttgaga ccccagtttt taaacattcg ggcatggaga
    11161 aagtactgat tgattgcgac aagctcattt cccaacagtt tgttgagata atttataact
    11221 ttagtatcac ctttcatttt ttgactcctt cgcttccact ctttgaagag tagaacggct
    11281 tacgagtaag tcaaaaaaca cactccgttt atgcggactc cttaaactcc ggcagctgca
    11341 ttaattcatc ctccatcact tcacgcgcgg cccggacaca tttaccgcac tgattgccga
    11401 ccggaataaa tttttttaat tgctggaacg agtgagggga aaactggcgc acagcctgac
    11461 gaattttttt atcgctgata ccattacaaa gacaaacgta catagagact cccgtatact
    11521 ttcttcaaaa ataaatgaaa atagttctta tttcaattac ggaaagactc ttttttaagc
    11581 aaagaataga aatatgaata aaaataatgg gctaccaaaa gtagcccatt gacaaaaaat
    11641 gcggcgatac tggaaggtat cgccaacaca tttattgctt agtaaacggc gcgaagattt
    11701 cgctaaatgc gtacttcggt gcctgttctt tagtcaggcc gtggaattcc ggagacaggt
    11761 tagtgccgcc cggcaggtcg cgcgcgattg accagatacc aaccataccg agattacgct
    11821 tctgcgcatc ctgcatgacc agacgagcat cagagagata gaacacctcg ccctgaacgt
    11881 cgttcacgcc aaccatcggc gtggtaccca tcatagcgtc aatttctgca tcgctcttat
    11941 tgggatggag gcctttcaat tgtgaatgca ggttggcaat cgcagacgtt gcacacttac
    12001 cgtgaatgtt ctggccttcg gtatttgcag actgacaaat cgcgttaccg tagtccattg
    12061 tcatcacgtt cacacccgcc agctcaacac ctttcgcttt ggcatcgctc aggacattca
    12121 tcccttccgg cgtcaggcca gtcggcagaa ttggcaaggt gtaccagata gcaatatctt
    12181 tgccttctga cttccattta tcctgcactt tcttcacagc aaggttacga cgttcaatag
    12241 atgcctgatc cgcaacccag gtgccttcga tatcgaagtc caggactttg aggttcaggt
    12301 tatcaacgat gtcataataa tgctgcatca gatcgtctac gttcttacag gaagcagcca
    12361 gcggagcgtt gttagcacca ccgatagaca gcatcacatc gccgccagcc tcacgcagag
    12421 ctttgatttt gctgtactga gcgtaattct gcataccgta agcggtaccc catgtcggca
    12481 gacaggtgtt cgcatcttta ctcaccacaa acgccagcgt gaagtggttg acgttatgat
    12541 tcttagccag cgcagccaga tccgggatcg tattcagcgt aaagtcgaca tacggtgcat
    12601 aaacgtgttt cggccacgct ttcttcggcg tacctacgcg ctctttggtt ccggtccagt
    12661 caacaaaaac acgccacggg ttgctgtcag aaggagaaac tttctgcact ttactctgag
    12721 aaatgtagtt cacaccgtta aagcgaatca gcgtatcgct ggcataaagc gtttctggat
    12781 taaactgcgg cgcattattc agctcttcgt tgctatagct ctgagcctta cctaacggct
    12841 tccacgggcg gctattgcta ccggtggcgt tttggttcgc caccagagca gggttgtcac
    12901 tttgcgtcca gaacagcgct tcataagcct gcccatcaac aacaacgcga tcacctttca
    12961 cgtacacagt ggatgcggac catgctttcg ccggttcgta tttcttccac gggttatcgc
    13021 cgcccgggac aaagccccat gcgtcttgat cgacagaagt tttataatgt gcatcgttat
    13081 aatctacgat gctgtccgcg ctgtacgctt tgctggcctg gaaaccatcc gcaacaatga
    13141 cgccgccgtt atcaatttca caggtagtcg ggttaccgta ctgactcaat tccgcagcgg
    13201 tagctgtacg ctcgagacgc catgggttat tggagttttc agcgctggta ccccgtgggc
    13261 aatctttaga cccaacccac caggcgttct tgtaaacatt accattgaaa ataacatggt
    13321 aaatttcgct accttcctgg ccgctccacg cttgcaatga gtaatcgtta acgctatccg
    13381 ctggtgttga tggctcgctg ttatccggag ttggggtcac cggtgtttcc ggagttggat
    13441 caaccggagt cacagcaccg ccattattat ccggcttaac ggaacaggag ccagggttac
    13501 catactggct gatttctgtt gccgttgccg cgcgaacata acgccatggg ttactggcat
    13561 cgttttcttt cgcatcgcct gggcaatttg ccgcgcctac ccaccaggcg ttcttataaa
    13621 tgccgccgtt atagataacg taccatgtag aagaaccttc ctgacctttc caggctacta
    13681 cagcatagtt gtcgtttgac ttatcagctg gggctggctg aacatcagga gccggtttaa
    13741 cgtccggtgc cggtttcaca tccggtgaag gctgtggtgc tgaagtacag gactgtggat
    13801 tactggtttc tgagatttcc gttgccgtag cggcacgaac ataacgccat gggttgctgg
    13861 catcattgct tttcgcatca cccggacagt tagaagaggc tacccaccag gcatttttat
    13921 ataccgcacc attaaagacg acataccagg tctgaccgcc ctgctgttta ttccaggcaa
    13981 ctacagaaga attagacgga acagtgccct gtgctggtgt agccgaaccg ccattagccg
    14041 gcgtattgga tgcaggcgta tttgaatttg aagaagatga gctgccgctc ttttcgcagg
    14101 aaagtgtatt gccaaactga ctaatttcag cagctgttgc ggtacgcttt aaacgccacg
    14161 ggttagttgc atcatttgct ttcgcttttc ccgggcaatt tgtagaagaa acccaccagg
    14221 cattttcata aattttgcca tcgaaaataa cctgatattt attaccacct tgttggttat
    14281 tccatgcttc cattgccaat gctggcagag cggaacacac cagccccata ccaatcatag
    14341 atttagtaaa tatatttaat ttcattataa tcccttgtga cgtaaaaact gcaaaacaaa
    14401 agtcctacgc aggaattatt tacgttttac gagaaccctt caatatgaat taaagtgcgg
    14461 cattaaaaat aagaaaaaag cctgacaaat gaagaatttt aaaaacagaa acattcatat
    14521 ttaaaatgtt aaattgaatt gatattttaa atatgaataa tttattcgtt ctgacagtac
    14581 gaataagata tgccgtcaac aaatgcaaaa agggcgccga agcgcccttt tcaattcaaa
    14641 actaattaac gtgtaattag cccagaactt tagc
//
LOCUS       DAZFZC010000111        14524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFZC010000111 DAZFZC010000000
VERSION     DAZFZC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.112"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..252
                     /locus_tag="ACHKXP_004102"
     CDS             49..252
                     /locus_tag="ACHKXP_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7648999.1"
                     /translation="MSNLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            252..1302
                     /locus_tag="ACHKXP_004103"
                     /pseudo
     CDS             252..1302
                     /locus_tag="ACHKXP_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1330..1419)
                     /locus_tag="ACHKXP_004104"
     tRNA            complement(1330..1419)
                     /locus_tag="ACHKXP_004104"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1383..1385),aa:Ser,seq:cga)
     gene            1513..2310
                     /gene="mtfA"
                     /locus_tag="ACHKXP_004105"
     CDS             1513..2310
                     /gene="mtfA"
                     /locus_tag="ACHKXP_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO7649000.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2411..2486
                     /locus_tag="ACHKXP_004106"
     tRNA            2411..2486
                     /locus_tag="ACHKXP_004106"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2444..2446,aa:Asn,seq:gtt)
     gene            2800..9876
                     /gene="yeeJ"
                     /locus_tag="ACHKXP_004107"
     CDS             2800..9876
                     /gene="yeeJ"
                     /locus_tag="ACHKXP_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HFO7649001.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSVTMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQYMTATLQDKNGNPLK
                     DKEITFSVPNDVASKFSISNGGKGMTDSNGVAIASLTGTLAGTHMIMARLANSNVSDA
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPSNHPVAGITVNFTMPQDV
                     AANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDSQPVTFVADKASA
                     QVVLQISKDEITGNGVDSATLTATVKDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNE
                     SGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGT
                     PQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTNEQGKATVTYTNT
                     RSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALFDTVSAGETTSLY
                     IEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASFTATKAGVYQLTA
                     TLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATVADTEGNAIANTE
                     VTFTLPEDVKANFTLSDGGKVITDAEGKAKVTLKGTKAGAHTVTASMTGGKSEQLVVN
                     FIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAVTFTLPADVSASF
                     TLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIADAGTAKLAS
                     LTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEI
                     LVTSTEVGLKTVSASLADKPTEVISRLLNASADVNSATITSLEIPEGQVMVAQDVAVK
                     AHVNDQFGNPVAHQPVTFSAEPSSQMIISQNTVSTNTQGVAEVTMTPERNGSYMVKAS
                     LPNGASLEKQLEAIDEKLTLTASSPLIGVYAPTGATLTATLTSANGTPVEGQVINFSV
                     TPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSST
                     AHVASFIADPSTIAATNTDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVT
                     DQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGD
                     YTDSAELRLVLHDISGNPIKVSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTG
                     GGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGA
                     YYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSY
                     VYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHCSSRGIGSLYSEWGDM
                     GHYTTDAGFQSNMYWSSSPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(10138..11190)
                     /locus_tag="ACHKXP_004108"
     CDS             complement(10138..11190)
                     /locus_tag="ACHKXP_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO7649002.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCFSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            11505..12821
                     /gene="shiA"
                     /locus_tag="ACHKXP_004109"
     CDS             11505..12821
                     /gene="shiA"
                     /locus_tag="ACHKXP_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO7649003.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGTLIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            12923..14377
                     /gene="amn"
                     /locus_tag="ACHKXP_004110"
     CDS             12923..14377
                     /gene="amn"
                     /locus_tag="ACHKXP_004110"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO7649004.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         3963 a         3445 c         3462 g         3654 t
ORIGIN      
        1 taattgccgc taaccgtata tagttagcgg cggttatgag atatagctat gagcaactta
       61 tttttaaccg aagatgaatt gctaatatta acgggctgca aatatgcaag ccaccagcga
      121 aaatggttaa cggaaaacgg gcttccgttc tataccaatc gtagtggcaa accgattgtc
      181 agccgggatc tatttacctg caataaaact ttaccaccac gcgaggtaga gccgaatttt
      241 ggtgcaatct aatgggaaga cgaaggaaaa atcctgaaca cgaaaaacta cctccaaatg
      301 tatacccaaa taaatatagt tatgtatgga aaccaacatc cagagaatct gtcacactaa
      361 ccgccatcaa ggatggttta gctgctttat ggaaaaagta tgaggaaact gtaaataatc
      421 gcgatcgtgc aatgacattc ggtcgcttgt gggaaaaatt cctcgccagc gcctattaca
      481 gtgacctcag tccaagaaca caaaaagatt atctgcaaca tcaaaaaaag ttgcttgccg
      541 tattcggtaa ggtgccggca gattccataa aaccagaaca catccgtcga tacatggaca
      601 agagagggga acagagtaaa acgcaagcca accatgaaaa aagcagtatg tcccgcgttt
      661 acagttgggg gtatgagcga gggtacgtga aggctaaccc atgtgcaggt gtaagtaaat
      721 tcaaggccaa aaaccgcgaa cgatatgtaa ccgacaaaga ataccaggca gtattaagcg
      781 ttgcacctct tcctgttttt atcgcaatgg aaattgccta tctgtgtgca gcgagggttt
      841 ccgatgtgtt atcgctgaaa tgggagcaga ttggaaacga cggaatcttt atccagcaag
      901 ggaaaacagg aaaaaaacag ataaaagcat ggagtccacg attacaggcg gcgatcgaaa
      961 aagcaaaaca gttaccaaca tccgcctatg taatcagtaa tcaatacggc aaccgatata
     1021 tgtacaaagg ctttaacgaa atgtgggtag aagcaagaaa tcgcgcaggc aaaatttcag
     1081 gtattttaac cgacttcacc tttcatgatc tgaaggcgaa aggaatttca gactatgaag
     1141 gaagcagtcg ggataagcaa cttttctctg gtcacaaaac cgaggggcaa gtgctaatct
     1201 atgacaggaa ggttaaagtt tcaccgacac ttgatgtccc gttacctgaa aatattccaa
     1261 gaaaatattc caagtaattc caagtgtgat ttttgtcact gacttaatga tgtataagtg
     1321 attgaatttt ggcggagaga gggggatttg aacccccggt ggagttgccc ccactccggt
     1381 tttcgagacc ggtccgttca gccgctccgg catctctccg ttcagatggt tgccatgatg
     1441 ccaggaaatt tggcatttta acagtccctg tccgtgcaat tttgttcaag tgacgagttt
     1501 gcgagcaaaa cgatgattaa gtggccctgg aaagtacaag aatcagcaca tcaaactgcc
     1561 cttccctggc aggaagcact atcgatcccc cttttaacgt gtctgacgga acaggaacaa
     1621 agcaaattag tcactcttgc cgaacgtttt ttacagcaaa agcggcttgt tcctttacag
     1681 ggctttgaac tggattcatt aagaagctgc cggatagcac ttctattttg cctacccgtt
     1741 ctggagttag gactggaatg gctggatggt tttcatgaag tcttaattta tcctgcgcca
     1801 tttgtggtcg atgatgaatg ggaagacgat atcggtctgg tgcataacca acgtattgtt
     1861 cagtcaggtc agagctggca gcaagggcct atcgttttga actggttgga tatacaagat
     1921 tcttttgatg cttctggttt taacctgatt attcatgaag tcgctcataa gctggacacc
     1981 cgtaacggcg atcgcgccag cggagttccc tttattccgt tgcgtgaggt tgctggctgg
     2041 gaacacgatc ttcatgctgc aatgaacaac attcaggaag aaatcgaatt ggttggcgag
     2101 aatgcggcga gcattgatgc ttatgcagcc agcgatcctg ctgaatgttt tgccgtactt
     2161 tctgaatatt tctttagcgc cccagaactt tttgctcctc gtttcccttc attgtggcaa
     2221 cgtttctgcc aattttatca acaagatcct ttgcagagac tgcatcacgc taatgataca
     2281 gactcgtttt cggcgacgaa tgttcattaa ttaacaactt tgcagattaa ttaaccaatt
     2341 gaaatgactt atgaaattta gtgttgacag acaaggtacc gctaagtaat attcgccccg
     2401 ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
     2461 tggttcgagt ccagtcagag gagccaaatt ctaaaaattc gcttttttag cgcaatgtca
     2521 ctgaccttag ttgaacattg ttttttaacg gatagcgggt ttttaacatc ttaagcgccc
     2581 tcgaccttta tggttgaggg cgttttgcta tgaacgccat caccattttc ccctcgatta
     2641 taaaacttga gttattcagt agtctcccct cttgcaactc acacccaaaa ctgcctaacg
     2701 aaaagttatt aattttcaat catattgcta tcagtattta cattttttcg ctgtgctaga
     2761 aagggcgcat ttatgttagc tcgttcaggg aaggtaagca tggctacgaa gaagagaagt
     2821 ggagaagaaa taaatgaccg acaaatatta tgcgggatgg gaattaaact acgccgctta
     2881 actgcgggta tctgtctgat aactcaactt gcgttcccta tggctgcggc agcacaaggt
     2941 gtggtaaacg ccgcaaccca acaaccagtt cctgcacaaa ttgccattgc aaatgccaat
     3001 acggtgccct acacccttgg agcgttggaa tcggcccaaa gcgttgccga acgtttcggt
     3061 atttcggtgg ctgagttacg caaactcaac cagtttcgta cgtttgctcg aggttttgat
     3121 aatgtccgcc agggtgatga actggatgtc ccggcacaag ttagtgaaaa aaaattaacc
     3181 ccgccgccgg gtaatagcag tgacaacctc gagcaacaga tagccagtac ttcacagcaa
     3241 atcgggtctc tgctcgccga agatatgaac agcgagcaag cggcaaatat ggcgcgtgga
     3301 tgggcctctt ctcaggcttc aggcgcaatg acagactggt taagccgctt cggtaccgca
     3361 agaatcacgc tgggcgtgga tgaggatttt agcctgaaga actcccagtt cgattttctc
     3421 catccgtggt atgaaacgcc tgataatctc tttttcagtc agcatactct ccatcgtact
     3481 gacgagcgta cgcagattaa caacggctta ggttggcgtc atttcactcc cacatggatg
     3541 tcgggcatca acttcttttt cgaccacgat cttagccgtt accactcccg cgccggcatt
     3601 ggcgcggagt actggcgcga ctatctaaaa ttaagcagta acggctattt gcgactgacc
     3661 aactggcgca gcgcacctga actggacaac gattatgaag cacgcccggc caatggctgg
     3721 gatgtacgcg cagaaagctg gctacccgcc tggccgcacc ttggcggtaa actggtctat
     3781 gaacagtatt atggcgatga agtggccctg ttcgataaag acgatcggca aagtaatcct
     3841 catgccataa ccgctggact taactatacc cccttcccgc tgatgacctt cagcgcggag
     3901 caacgccagg gtaaacaggg cgaaaatgac acccgttttg ccgtcgattt tacctggcaa
     3961 cctggcagcg caatgcagaa acagcttgac ccgaatgaag tcgctgcacg gcgtagcctt
     4021 gcaggcagcc gttatgatct ggtggatcgc aacaacaata tcgttctgga atatcgcaaa
     4081 aaagaactgg ttcgcctgac cctgacagac cccgtgacag ggaagtcagg agaagtgaaa
     4141 tcactggttt cgtcgctaca aaccaaatat gccctgaaag gctataacgt cgaagccacc
     4201 gcactggaag ctgccggtgg caaagtggtc acaacgggta aagatattct ggttaccctg
     4261 ccggcttacc ggttcaccag tacgccagaa accgataaca cctggccgat tgaagtcacc
     4321 gccgaagatg tcaaaggcaa tttgtcgaat cgtgaacaga gcatggtggt cgttcaggca
     4381 cctacgctaa gccagaaaga ttcctcggta tcgttaagta cccaaacatt gaacgcggat
     4441 tcccattcaa ccgccacact gacttttatt gcgcatgatg cagcaggtaa tcctgttgtc
     4501 gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca tcaccctttc tgactggaaa
     4561 gataatggtg acggaagcta tacccagatc ctgaccacag gagcgatgtc tggcacgctg
     4621 acgctgatgc cacagctgaa tggtgtggat gcggctaaag cccccgccgt ggtgaatatc
     4681 atttctgttt cgtcatcccg aactcactcg tcaattaaga ttgataagga ccgttatctc
     4741 tccggcaatc ctatcgaggt gacggtagaa ctgagagatg aaaatgacaa acctgttaag
     4801 gaacaaaaac agcaactgaa taacgcagtc agcatcgaca acgtgaaacc aggagtcact
     4861 acagactgga aagaaaccgc agatggcgtc tataaggcga cctataccgc ctataccaaa
     4921 ggcagtggac ttactgcgaa gctattaatg caaaactgga atgaagattt gcataccgct
     4981 ggatttatca tcgacgccaa cccgcagtca gcgaaaattg cgacattatc tgccagcaat
     5041 aatggtgtgc tcgccaatga gaatgcagca aacaccgtct cggtcaatgt cgctgatgaa
     5101 ggaagcaacc caatcaatga tcataccgtc acgtttgcgg tattaagcgg atcggcaact
     5161 tccttcaaca atcaaaacac cgcaaaaacg gatgttaatg gtctggcgac ttttgatctg
     5221 aaaagtagta agcaggaaga caacacggtt gaagtcaccc ttgaaaatgg cgtgaaacaa
     5281 acgttaatcg tcagttttgt cggcgactcg agtaccgcgc aggttgatct gcagaagtcg
     5341 aaaaatgaag tggttgctga cggcaatgac agcgtcacaa tgaccgcgac cgtccgggat
     5401 gcaaaaggca acctgctcaa tgacgtcatg gtcactttca atgttaattc agcagaggcg
     5461 aaactgagcc aaaccgaagt gaatagccac gacgggatcg ccacagctac gctgaccagt
     5521 ttgaaaaatg gtgattatag ggttacggcc tctgtgagct ctggttccca ggctaatcaa
     5581 caggtgaatt ttatcggtga tcaaagtact gctgccctga ccctcagtgt gccttcaggt
     5641 gatatcaccg tcaccaacac agctccgcaa tatatgactg caaccttgca ggataaaaat
     5701 ggcaacccac taaaagataa agaaatcacc ttctctgtgc caaacgacgt cgcaagtaag
     5761 ttctcgatta gcaacggagg aaaaggcatg acggatagta acggggttgc aatcgcctcc
     5821 ctgaccggca cgttagcggg cacgcatatg atcatggctc gtctggctaa cagcaatgtc
     5881 agcgatgcac agccaatgac gtttgtggcg gataaagaca gagcggttgt cgttttgcaa
     5941 acatcgaaag cggaaatcat tgggaatggc gtggatgaga caactctgac agcaacagtg
     6001 aaagatccgt cgaatcatcc ggtggcgggg ataacggtaa acttcaccat gccacaggac
     6061 gttgcggcaa actttaccct tgaaaataac ggtattgcca tcactcaggc caatggggaa
     6121 gcgcatgtca cgctgaaagg taaaaaagcg ggcacgcata cggttaccgc aacgctgggt
     6181 aataacaata ccagtgattc gcagccggta acatttgtgg cggacaaagc ctcggctcag
     6241 gttgtcctgc agatatcaaa agatgagatc acaggtaatg gcgtcgatag cgcaacgcta
     6301 actgcaacgg ttaaagatca gttcgacaat gaggtgaata atcttccggt aacattcagc
     6361 tcagcctctt caggactcac cctgaccccg ggagtaagta ataccaacga gtctggcatc
     6421 gcgcaggcca ctctcgcagg cgttgccttt ggtgagaaga cggttactgc atcactggct
     6481 aataatggtg ccagcgacaa caaaactgtg cattttattg gcgacacagc ggcggcaaaa
     6541 attatcgagt tgacgcctgt cccagacagc ataatcgccg gtaccccgca gaacagctcc
     6601 ggcagcgtca tcaccgccac agtcgttgat aataatggct ttccggtgaa aggtgtgact
     6661 gtgaacttca ccagcaacgc agcgacagcc gaaatgacga acggcggtca agccgtgacg
     6721 aacgaacagg gtaaggctac cgtcacttat accaataccc gctcctcgat agaatcagga
     6781 gcgagaccgg ataccgttga ggccagtctg gaaaatggta gctccacgct tagcacatca
     6841 attaatgtca acgctgatgc gtctacggca catctcacct tgctacaggc actttttgat
     6901 acagtctccg caggcgagac aaccagtctg tatattgagg tgaaggataa ttacggcaac
     6961 ggtgtccccc agcaggaggt aaccctcagc gtttcaccaa gtgaaggcgt gacccccagt
     7021 aataacgcta tatatactac caaccacgac ggcaattttt acgcaagctt taccgctaca
     7081 aaagccgggg tttatcaatt gacggcaacc ctcgaaaatg gcgattcgat gcaacaaaca
     7141 gtgacctatg tgccgaacgt cgcgaatgct gaaatcacgc tggcagcctc gaaggatccg
     7201 gtgattgccg acaataacga tctcacgaca ctaacagcaa cagtcgctga tacagagggc
     7261 aatgcgatag ccaacactga ggtaacattt actctgccgg aagatgtgaa ggcgaacttc
     7321 acgctgagcg atggcggtaa agtgattact gatgctgaag gcaaagcgaa agtcacgctg
     7381 aaaggtacaa aagcaggcgc tcatactgtt acagcatcga tgactggcgg taagagtgag
     7441 cagttggtgg tgaactttat tgcggatacg ctcactgcgc aggttaatct taacgttacc
     7501 gaggacaatt ttatcgctaa taacgtcggg atgaccaggc tgcaggcaac agtgactgat
     7561 ggaaacggca acccgttagc caatgaggcg gtgacattca cgctaccggc agatgtgagc
     7621 gcaagcttta ctctcggaca aggcggttcc gccattactg atatcaacgg caaggctgaa
     7681 gttacactga gcggtacaaa atccggcacc taccccgtga cagttagcgt gaacaattat
     7741 ggtgtcagtg atacgaaaca ggtgactttg attgccgatg ctggtaccgc aaaactagcc
     7801 tccttaacct ctgtatactc attcgtcgtc agcacgaccg agggcgcaac catgacggca
     7861 agcgtcactg acgctaacgg caacccggta gaaggcataa aagttaattt ccgcggaacc
     7921 tccgtcacgc taagcagcac cagcgttgaa acggatgatc ggggtttcgc tgaaattctt
     7981 gtgacaagca ccgaggtcgg actgaaaaca gtttcagcct ctctggcaga taaacctact
     8041 gaagtcatct cgcgattact gaatgccagt gcagatgtta attctgcgac gattaccagt
     8101 ctggagatac cggaaggtca ggtaatggtc gcacaagacg tagcagttaa agctcacgtt
     8161 aacgaccagt ttggcaaccc ggttgcgcat caacccgtga cattcagtgc agagccatcc
     8221 tcgcaaatga tcatcagcca gaatacggtc tctactaata cgcagggtgt agccgaggtc
     8281 accatgacgc ccgaaagaaa cggttcgtat atggtgaaag catccctgcc gaatggagcc
     8341 tcacttgaga aacaactgga ggctattgat gaaaaactga cactcacggc gtccagtccg
     8401 cttatcggtg tctatgcccc tacaggcgct actctgacgg caacgctaac ctctgcaaat
     8461 ggcactccag tggagggtca ggtcatcaac tttagcgtaa cgccagaagg ggcgacgtta
     8521 agtggcggaa aagtgagaac taactcttca ggtcaggctc cagtcgtttt gaccagcaat
     8581 aaagtcggta catatacggt gactgcatct ttccataacg gcgtaacaat acagacacag
     8641 acaaccgtga aagtcactgg caactcaagc accgcccatg ttgctagctt tatcgctgat
     8701 ccatcgacta tcgccgccac caacactgat ttaagtacct taaaggcaac ggttgaggat
     8761 ggcagtggta acctgatcga aggtctcact gtgtacttcg ccttaaaaag cggctctgcc
     8821 acattaacgt cattaacagc ggtgaccgat caaaacggaa tcgcgacaac aagcgtgaaa
     8881 ggagcgatga caggtagcgt cacggtaagc gcagtcacga ccgctggtgg aatgcaaaca
     8941 gtagatataa cgctggtggc tggcccggca gacacctcgc agtccgtcct taagagcaat
     9001 cggtcatcac tgaaagggga ctataccgat agtgctgaat tacgtcttgt tctgcacgat
     9061 atatcaggca atccgatcaa agtttctgaa gggatggaat ttgtgcaatc aggtactaac
     9121 gtgccctata taaaaattag cgcaattgat tacagtctaa atatcaacgg tgattacaaa
     9181 gccactgtta caggaggcgg agagggtatc gcaacgctga tccctgtatt gaatggtgtt
     9241 catcaagctg gtctgagtac cacaatacaa ttcactcgcg cagaagacaa aataatgagc
     9301 ggtacagtat cagtcaatgg tactgaccta ccgacaacta cattcccttc gcaggggttc
     9361 accggggcgt attatcagtt gaataatgac aactttgccc caggaaaaac ggcggctgat
     9421 tatgagtttt caagctctgc ctcctgggtc gatgttgatg ctaccggtaa agtgacattt
     9481 aaaaatgtcg gcagcaattc ggaaaggatt acggcgacgc caaaatcagg aggccctagc
     9541 tatgtatacg aaatccgtgt gaagagttgg tgggtgaacg ccggcgaggc tttcatgata
     9601 tacagccttg ctgaaaattt ttgcagcagc aatggctaca cgctccccag agcaaactat
     9661 ttaaaccact gtagttcccg aggcatcggg tcactgtaca gtgaatgggg agatatgggg
     9721 cattacacga ctgacgctgg ttttcaatca aatatgtatt ggtcatctag tcccgcaaac
     9781 tcaagcgaac aatacgtagt ttccctggca acaggtgatc aaagcgtatt tgaaaagctt
     9841 gggtttgctt atgcgacatg ttataaaaac ctgtgatttt ccgttataat ttcttaaaga
     9901 gtgttccatt cggggcactc ttttttacat tccctctaca gaactagccc ttctctcaca
     9961 gaaaatgaat aaataaaaat gcggcaccgc cagaatcgcg ttcgatgtga ctatgatcat
    10021 tttcggtgag tactgctttg atctaaaggc tggttttctg tgatgattga ccagcctcag
    10081 agtattgatg acaccaccgt tcataacaga tcggcattac tatgcataag tactgtctca
    10141 tattctcgcc aaaacacttt ttaatgcctt tgcgaattca tccagactta tattgtcaat
    10201 tttttgccca acgacgactg actccgagac atcggccatt gcgggccatc gcgttttgta
    10261 atcagcataa attgcaacaa caggggtatt ataggcactt gccatatgta atattgaggt
    10321 gtcaggagtt attgcgatat aagcatcttt tatgattgct gctgaacgtt taatcgaggg
    10381 tggtaaggat aaacggtaga cattattata acaatccact aatgccctgg ctttatcttc
    10441 acctttgggt ccatgaacga tcactattgg tatatctgtt tcactttgaa tttttgctat
    10501 tagattttct gcaatcgata atgaaaatgt acgttcctgc gagctacctt ctaaattgaa
    10561 cgcaatgtaa gatcctaacg atcgcatttc ctcgcgcacc tcatcgagta catcctcact
    10621 tagtggcaat tcatatattg gcctgactgc tggaaaaccc gcctcacgca tcataaatgc
    10681 ccatgtcata ggtacgggag cccggagttt ctgatccata cgggacgcgt tcttgcacaa
    10741 aggggagaag caattcatgg ttataccaac aacctgaaaa ttcgtttttg ctttcaactg
    10801 actgataaat aacatcgttt tcaggttctt tttacgcatc ccctcaatgc aaagatccgg
    10861 cgtaccgtat tgctgtgtta tgttctttgc taaatctttt atttctttta atgttgcgtg
    10921 atcctgcata gtcattgtga ctaatgttaa tttagtctgt tcaagtttaa gcgcattaaa
    10981 gacttctaaa ttaattgtcg acgttacaat taaaagatgc ttaattttat gcaattcaag
    11041 cgcccgaata acaggaaaga tggccatagc atcgccaatc tgatcgggaa tatggatgac
    11101 aacaaagtct gttttttcaa tattgaaatt ataagcttta taatcgtagt aactaaatgc
    11161 aatacgtctc aacaatgatg ctaaaaacat acctaacctc gcctccctac tggttataat
    11221 gcaatgcagt ctatcagact catcagggtg ccattttgtg catatgcgga cttttatgtt
    11281 tcatatctct aacctgtggg tcctctgctt aatccttaaa caacaccagc aactcctgcg
    11341 ctttcatctt ccatcgaatt tttcatgttg ccgctaatca gccataaaat catttgcaga
    11401 tgcgctctgt cgaggtagtc tcataaggtt cgtttataga tcgacggcaa tgtgagttac
    11461 cttttccata ctaattataa aaagacagta caaacaggat cattatggac tccacgctca
    11521 tctccactcg tcccgatgaa gggacgcttt cgttaagtcg cgcccgacga gctgcgttag
    11581 gcagcttcgc tggtgccgtc gtcgactggt atgatttttt actctatggc atcaccgccg
    11641 cactggtgtt taatcgcgag tttttcccgc aagtaagccc ggcgatggga acgctcgccg
    11701 catttgctac ctttggcgtc ggatttcttt tccgtccgct cggcggtgtc attttcggtc
    11761 actttggcga ccgactggga cgtaagcgca tgttaatgct gaccgtctgg atgatgggca
    11821 tcgcgacagc cttgattggt attcttcctt cattctcgac cattgggtgg tgggcaccta
    11881 ttttgctggt gacactgcgt gccattcagg gatttgcagt cggcggcgaa tggggaggcg
    11941 cggcgttgct ttccgttgaa agtgcaccga aaaataaaaa agccttttac agtagcggtg
    12001 tacaagttgg ctacggtgta ggtttactgc tttcaaccgg actggtttca ttgatcagta
    12061 tgatgacgac tgacgaacag tttttaagct ggggctggcg cattcctttc ctgtttagca
    12121 tcgtactggt actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg
    12181 aacaacagca acattatcaa gctgccgcga aaaaacgcat cccggttatc gaagcgctgt
    12241 tacgacatcc cggtgctttc ctgaagatta ttgcgctacg actgtgcgaa ttgctgacga
    12301 tgtacatcgt tactgccttt gcacttaatt attcaaccca gaatatgggg ctaccgcgcg
    12361 aacttttcct taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt
    12421 ttgcctggct tgccgatcgt tttggtcgcc gtagggttta tatcacaggt acgttaatcg
    12481 gaacgttgag cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag
    12541 ttttcttctc cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac
    12601 cgatgtttac cgaaatgttt ggtgccagtt atcgctatag tggcgctgga gtcggttatc
    12661 aggttgccag tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt
    12721 actttgccgg gaactggcat agcgtcgcca tttatttgct ggctggatgc ctgatttccg
    12781 caatgaccgc tttgttgatg aaagacagtc aacgcgcttg atagcctggc gaagatcatc
    12841 cgatcttcgc cttacacttt tgtttcacat ttctgtgaca tactatcgga tgtgcggtaa
    12901 ttgtatggaa caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc
    12961 actggataaa ctcgacgcgc tgtatgagca atctgtagtc gcattacgca acgccattgg
    13021 caactatatt acaagtggcg aattacctga tgaaaacgcc cgcaaacaag gtctttttgt
    13081 ctatccatca ctgaccgtaa cctgggacgg tagcacaacc aatcccccca aaacgcgcgc
    13141 atttggtcgc tttacccacg caggcagcta caccaccacg attactcgcc ctactctctt
    13201 tcgttcgtat cttaatgaac aacttacgtt gctgtatcag gattatggtg cgcatatctc
    13261 agtgcaaccc tcgcagcatg aaatccctta tccttatgtc atcgatggct ctgaattgac
    13321 acttgatcgc tcaatgagcg ctgggttaac tcgctacttc ccgacaacag aactggcgca
    13381 aattggcgat gaaactgcag acggcattta tcatccaact gaattctccc cgctatcgca
    13441 ttttgatgcg cgccgcgtcg atttttccct cgcacggttg cgccattata ccggtacgcc
    13501 agttgaacat tttcagccgt tcgtcttgtt taccaactac acacgttatg tggatgaatt
    13561 cgttcgttgg ggatgcagcc agatcctcga tcctgatagt ccctacattg ccctttcttg
    13621 tgctggcggg aactggatca ccgccgaaac cgaagcgcca gaagaagcca tttccgacct
    13681 tgcatggaaa aaacatcaga tgccagcatg gcatttaatt accgccgatg gtcagggtat
    13741 tactctggtg aatattggcg tgggaccgtc aaatgctaaa accatctgcg atcatctggc
    13801 agtgctacgc ccggatgtct ggttgatgat tggtcactgt ggcggattac gtgaaagtca
    13861 ggccattggc gattatgtac ttgcacacgc ttatttacgc gatgaccacg ttcttgatgc
    13921 ggttctgccg cccgatattc ctattccgag cattgctgaa gtgcaacgtg cgctttatga
    13981 cgccaccaag ctggtgagcg gcaggcccgg tgaggaagtc aaacagcggc tacgtactgg
    14041 tactgtggta accacagatg acaggaactg ggaattacgt tactcagctt ctgcacttcg
    14101 ttttaactta agccgggccg tagcaattga tatggaaagt gcaaccattg ccgcgcaagg
    14161 atatcgtttc cgcgtgccat acgggacact actgtgtgtt tcagataaac cgttgcatgg
    14221 cgagattaaa cttcccggcc aggctaaccg tttttatgaa ggcgctattt ccgaacatct
    14281 gcaaattggc attcgggcga tcgatttgct gcgcgcagaa ggcgaccgac tgcattcgcg
    14341 taaattacga acctttaatg agccgccgtt ccgataataa gttctgaacc ttcttatcag
    14401 aaatagatgt gaaggagtaa gtaagacacc tggcaaatag cctgcaataa tcgtgggctg
    14461 tttgcttcct tgggcggata cgagttttat tatcgtctta atgatttcca catattaaaa
    14521 gcaa
//
LOCUS       DAZFZC010000112        14513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFZC010000112 DAZFZC010000000
VERSION     DAZFZC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.113"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1777)
                     /locus_tag="ACHKXP_004111"
     CDS             complement(<1..1777)
                     /locus_tag="ACHKXP_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFO7649005.1"
                     /translation="MKNDNAVQHNNQTASEQTLSPDEGHVLHKVRDPVCGMAILPDRA
                     HSSIRYQDHQLYFCSASCESKFKAHPDRYLTEDASEHSHHHHHDHHEVSPDQIKQPHH
                     QAEKENSEGVWTCPMHPEIRRSGPGSCPVCGMALEPLVATASTGPSDELHDMTRRFWL
                     GLLLAFPVLVLEMGSHLFPELRNTVPPQYNTWLQLLLASPVVLWCGWPFFARAGMSLR
                     NRSLNMFTLVAMGTGVAWVYSVIATVFPSWFPASFRNMDGLVAVYFEAAAVITVLVLL
                     GQVLELRAREQTSGAITALLNLAPKTARRLDHDGHETDINAEDVLPGDKLRIRPGESI
                     PVDGIVIEGKTTVDESMVTGESMPVTKTEGDPVIGGTINQTGSLIIRAEKVGDETMLS
                     RIVQMVADAQRSRAPIQRMADSVSGWFVPLVILIAVVAFVIWSVWGPEPRMAHGLIAA
                     VSVLIIACPCALGLATPMSIMVGVGKGAQAGVLIRNAEALERLEKVDTLVVDKTGTLT
                     EGSPTVTGIISLNPGGETSLLRVTAAVEKGSQHPLGMAVVKAAQEKGIAIPAVTHFDA
                     PSGKGVSGDVEGQRVVIGNELAMQEN"
     gene            complement(1904..2344)
                     /locus_tag="ACHKXP_004112"
     CDS             complement(1904..2344)
                     /locus_tag="ACHKXP_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HFO7649006.1"
                     /translation="MKKVVLMALALGLSLPAMASEKVIDMYKSENCGCCSLWGKAMEK
                     DGFEVRTHVMNDQALSALKEKHAIPAGLRSCHTAVAGNLIIEGHVPATTIHKAMQSGS
                     GIYGLATPGMPAGSPGMEMGARKEAYDVIAFSPDGSKKVFQRIE"
     gene            complement(2430..5576)
                     /gene="silA"
                     /locus_tag="ACHKXP_004113"
     CDS             complement(2430..5576)
                     /gene="silA"
                     /locus_tag="ACHKXP_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit SilA"
                     /protein_id="HFO7649007.1"
                     /translation="MIEWIIRRSVANRFLVMMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     LYWARSRVLEYLNQVQGKLPAGVSSEIGPDATGVGWIFEYALVDRSGKHDLSELRSLQ
                     DWFLKFELKTIPNVAEVASVGGVVKQYQIQVNPVKLSQYGISLPEVKQALESSNQEAG
                     GSSVEMAEAEYMVRASGYLQSIDDFNNIVLKTGENGVPVYLRDVARVQTGPEMRRGIA
                     ELNGQGEVAGGVVILRSGKNARDVITAVRDKLETLKASLPEGVEIVTTYDRSQLIDRA
                     IDNLSSKLLEEFIVVAIVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWDHQHPGEQIDNATRWKVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYSMAGAAALAIIVIPILMGFWIRGK
                     IPAETSNPLNRVLIKAYHPLLLRVLHWPKTTLLVAALSIFTVIWPLSQVGGEFLPKIN
                     EGDLLYMPSTLPGVSPAEAAALLQTTDKLIKSVPEVASVFGKTGKAETATDSAPLEMV
                     ETTIQLKPEDQWRPGMTIDKIIEELDRTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLSDIDATAQSIEAVAKTVPGVVSALAERLEGGRYIDVDINREKASRYGMTV
                     GDVQLFISSAIGGATVGETVEGVARYPINIRYPQDYRNSPQALKQMPILTPMKQQITL
                     GDVADIKVVSGPTMLKTENARPASWIYIDARGRDMVSVVNDIKTAISQKVKLRPGTSV
                     SFSGQFELLEHANKKLKLMVPMTVMIIFILLYLAFRRVDEALLILMSLPFALVGGIWF
                     LYWQGFHMSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAHPELSRKETFTPEGLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLIWLRRHKKSVS"
     gene            complement(5587..6879)
                     /gene="silB"
                     /locus_tag="ACHKXP_004114"
     CDS             complement(5587..6879)
                     /gene="silB"
                     /locus_tag="ACHKXP_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit SilB"
                     /protein_id="HFO7649008.1"
                     /translation="MASLKIKYAAIIISSLIAGGLISVTAWQYVNSAQKTEKTEQKAP
                     ERKVLFWYDPMKPDTKFDKPGKSPFMDMDLVPKYADDSGDKSSGGIRIDPTQVQNLGL
                     KTQKVTRGMLNYSQTIPANVSYNEYQFVIVQARSDGFVEKVYPMTIGDHVKKGTPLID
                     ITIPDWVEAQSEFLLLSSTGGTSTQIKGVLERLRLAGMPEEDIQRLRSTRSIQTRFTI
                     KAPIDGVITAFDLRTGMNISKDKVVAQIQGMDPVWISAAVPESIAYLLKDTSQFEISV
                     PAYPDKTFHVEKWNILPSVDQTTRTLQVRLQVSNKDEFLKPGMNAYLKLNTRSQEMLL
                     IPSQAVIDTGKEQRVITVDDEGKFVPKQIHVLHESQQQSGIGSGLNEGDTVVVSGLFL
                     IDSEANITGALERMRHPEKTESSMPAMSDQPVNMHSGH"
     gene            complement(6993..7346)
                     /gene="cusF"
                     /locus_tag="ACHKXP_004115"
     CDS             complement(6993..7346)
                     /gene="cusF"
                     /locus_tag="ACHKXP_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux system protein CusF"
                     /protein_id="HFO7649009.1"
                     /translation="MRNSLKAVLFGAFSVMFSAGLHAETHQHGDMNTASDASVQQVIK
                     GTGVVKDIDMNTKKITISHEAIPAVGWPAMTMRFTFVNADDAINALKTGNHVDFSFIQ
                     QGNISLLKSINVTQS"
     gene            complement(7374..8759)
                     /gene="silC"
                     /locus_tag="ACHKXP_004116"
     CDS             complement(7374..8759)
                     /gene="silC"
                     /locus_tag="ACHKXP_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel SilC"
                     /protein_id="HFO7649010.1"
                     /translation="MFKLKLLSISTIFILAGCVSLAPEYQRPAAPVPQQFSLSHNSLT
                     PAVNGYQDTGWRNFFVDPQVTRLIGEALTNNRDLRMAALKVEEARAQFNVTDADRYPQ
                     LNASSGITYSGGLKGDKPTTQEYDARLELSYELDFFGKLKNMSDADRQNYFASEEARR
                     AVHILLVSNVSQSYFSQQLAYEQLRIARETLKNYQQSYAFVEQQLVTGSTNVLALEQA
                     RGQIESTRAEIAKREGDLAQANNALQLVLGTYRALPSEKGMKGGEIAPVKLPPNLSSQ
                     ILLQRPDIMEAEYQLKAADANIGAARAAFFPSITLTSGLSASSTELSSLFTSGSGMWN
                     FIPKIEIPIFNAGRNKANLKLAEIRQQQSVVNYEQKIQSAFKDVSDTLALRDSLSQQL
                     ESQQRYLDSLQITLQRARGLYASGAVSYIEVLDAERSLFATQQTILDLTYSRQVNEIN
                     LFTALGGGWVE"
     gene            8949..9629
                     /gene="silR"
                     /locus_tag="ACHKXP_004117"
     CDS             8949..9629
                     /gene="silR"
                     /locus_tag="ACHKXP_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver response regulator transcription
                     factor SilR"
                     /protein_id="HFO7649011.1"
                     /translation="MKILIVEDEIKTGEYLSKGLTEAGFVVDHADNGLTGYHLAMTAE
                     YDLVILDIMLPDVNGWDIIRMLRTAGKGMPVLLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGNTMITESQFKVADLSIDLVSRKVSRAGNRIVLTSKEFSLLE
                     FFIRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRAKIDNDYETKLIQTVRGVG
                     YMLEVPDA"
     gene            9622..11103
                     /gene="silS"
                     /locus_tag="ACHKXP_004118"
     CDS             9622..11103
                     /gene="silS"
                     /locus_tag="ACHKXP_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005763473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver sensor histidine kinase SilS"
                     /protein_id="HFO7649012.1"
                     /translation="MHSKPSRRPFSLALRLTFFISLSTILAFIAFTWFMLHSVENHFA
                     EQDVSDLQQISTTLNRILQSPVDPDDKKISKIKESIASYRNVALLLLNPRGEVLFSSA
                     QGAALRPAVNSADFSEHSRARDVFLWTVEDPAGPMDTGSEMKMETYRIIASSGQAIFQ
                     GKQQNYVMLTGLSINFHLHYLDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVS
                     NAIKNITSENLDARLEPTRVPIELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRT
                     PITNLVTQTEIALSQDRTQRELEDVLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDR
                     VMFDLRAEVMKVFEFFEAWAEERNITLKFNGMPCLVEGDPQMFRRAINNLLSNALRYT
                     PEGQAITVSIREQESFFDLVIENPGKPIPEEHLSRLFDRFYRVDPSRQRKGEGSGIGL
                     AIVKSIVEAHHGRVQVESDVHSTRFILSVPRLEKMIPDTQCWE"
     gene            11348..11779
                     /gene="silE"
                     /locus_tag="ACHKXP_004119"
     CDS             11348..11779
                     /gene="silE"
                     /locus_tag="ACHKXP_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="silver-binding protein SilE"
                     /protein_id="HFO7649013.1"
                     /translation="MKNIVLASLLGFGLISSAWATETVNIHERVNNAQAPAHQMQSAA
                     APVGIQGTAPRMAGMDQHEQAIIAHETMTNGSADAHQKMVESHQRMMGSQTVSPTGPS
                     KSLAAMNEHERAAVAHEFMNNGQSGPHQAMAEAHRRMLSAG"
     gene            11927..12277
                     /locus_tag="ACHKXP_004120"
     CDS             11927..12277
                     /locus_tag="ACHKXP_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFO7649014.1"
                     /translation="MKARNTLFAVLMLSLPAISAEHSEMKMTDMSTSASSQEYMAGMK
                     DMHDKMMAAVNESDPDKAFAKGMVAHHEGAIAMAETELKYGKDPKMRKLAQDIIKAQK
                     GEIEQMNKWLDSQK"
     gene            complement(12446..14272)
                     /locus_tag="ACHKXP_004121"
     CDS             complement(12446..14272)
                     /locus_tag="ACHKXP_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7649015.1"
                     /translation="MRLRKLKLKNFRGYRNSTEIIIDESMTGIVGRNDFGKSTLLEAL
                     AIFFETEGMKADKNDMNCFSLREGDGRFEIACEFDDLPDFIMIDDRVQTTLASEHLLN
                     EDGNFEIVKTFKATTSGKPEQTCIRCIHPDEEPLRNLLGMKISELKAVGKEVEKNVAD
                     KRTASLWRQAIREAAAPYTCSEIMLDVDKEFGTDTKSLWGKILDLLPTYAIFKADRES
                     SDGDSEAKNPLQQAVKDAQAALQDKITALENEIQDSVLDVAQRTLDKLREMAPELASE
                     LTPRFKEKPKWTFNFTLDGENGIPINKRGSGIRRLILLNFFRAEAEKNVAGTPRNVIY
                     AIEEPETSQHPNYQMMLMKALLALAGQPHRQIIVTTHVPALAGLIPVEGVRYVTRNEA
                     GEPVVKMPDDAVLKEATESLGVLPETGMERAQGIVLVEGKSDVTFLRHAASSLKQSGA
                     LPASLEDVKIVPVLIGGCGSVKHWVTLNLAKDLGLPWCVFLDSDIGGDPAQVLSIQKR
                     KKEVEEAGKVFFATRKREIENYLCPDLIEEITGVAVTFTDTCDAKKIIGRAVGMKPDN
                     VLDKFWPQMTSERIISRSTYHDGTQERSELVEILSDIVSMTR"
BASE COUNT         3531 a         3807 c         3543 g         3632 t
ORIGIN      
        1 tgttttcctg catagccagt tcattaccaa taacaacccg ttgaccttcg acatcgcctg
       61 agacaccttt acccgacggt gcatcgaaat gagtgactgc gggtattgcg atcccctttt
      121 cctgtgctgc tttaactact gccataccca gcggatgctg cgagcctttt tccactgcgg
      181 ctgttacacg caaaagagat gtttccccac ccggattgag actgataatc cctgtcaccg
      241 taggcgaacc ttccgtgagc gtgcctgttt tgtcgacaac cagcgtgtcc actttttcaa
      301 gacgctcaag ggcttcggca ttcctgatta acaccccggc ctgggctcct ttgcccaccc
      361 ccaccattat cgacatcggc gtggccagcc ccagcgcgca gggacaggca ataatcagga
      421 ccgacacagc cgcaatgaga ccgtgcgcca tcctgggctc gggcccccag acagaccaga
      481 tcacgaaagc aacaaccgcg ataagtatca ccagaggaac aaaccagcct gaaacgctgt
      541 cagccattct ctggatgggg gcacgcgaac gctgtgcatc agcgaccatc tgaacaattc
      601 gtgagagcat tgtttcatca ccgactttct ctgcacggat gatgagactc cctgtctgat
      661 taatggtccc cccgatgaca gggtcaccct ccgttttggt aaccggcata gattccccgg
      721 tcaccatcga ttcatcaacg gttgttttgc cttcgatcac gataccgtcg accggaatac
      781 tctctccagg tctgatgcgg agcttatcgc caggcaggac atcttccgca ttaatatccg
      841 tttcatgacc gtcatgatcc agccgtctgg cggttttggg ggcaaggttc agaagcgcag
      901 taatggcgcc tgaggtttgt tcccgtgccc gcagctcaag aacctgtccc agcagaacaa
      961 gcaccgtaat aactgctgcg gcttcaaaat aaacggccac caggccatcc atgtttctga
     1021 acgatgcagg aaaccaggag gggaagacgg ttgcaatgac gctgtaaacc caggctacgc
     1081 cggtccccat tgcaacaagg gtaaacatat tcagggagcg gttacgtaac gacattccgg
     1141 cccgggcgaa gaatggccag ccacaccaca acacgacagg ggaggccaga agcagctgca
     1201 gccatgtgtt gtactgtggc ggtactgtat tcctcaactc gggaaacaga tgagatccca
     1261 tttcgagtac cagaaccgga aacgccagca acaaccccag ccagaagcgt cttgtcatgt
     1321 cgtgaagttc atcactcggc cccgtggatg ccgtagctac gagcggctcc agtgccattc
     1381 cacagacagg acagcttccg ggaccactgc ggcgtatctc cgggtgcatc ggacatgtcc
     1441 acacaccttc agaattctct ttttccgcct ggtggtgagg ctgttttatc tgatcagggc
     1501 tgacttcgtg atgatcgtgg tgatggtgat gggaatgttc actggcatct tcggtaagat
     1561 aacgatcggg atgggcttta aatttactct cacagctggc ggagcagaaa taaagttgat
     1621 ggtcctggta tcgaatgctg ctgtgcgccc tgtcgggcag gatggccatc ccgcacacgg
     1681 gatctctcac cttatgcaat acgtggccct cgtccgggga taatgtctgc tcagaagcag
     1741 tctggttgtt gtgttgcact gcattgtcat ttttcacagt aactctcctt atgcataccg
     1801 aagcatcttc tgtcttcagg gtatgtcatg gatgcgatta ccacgaatat cgccggcaca
     1861 ggagtgcctg ctaccggcgt cccgttatca gccgttccgc tgactattcg attcgctgga
     1921 agactttttt acttccatcc ggtgagaatg cgataacatc gtaagcctct tttcgggctc
     1981 ccatctccat tccaggactt cctgctggca taccgggggt ggcgagaccg tatatacctg
     2041 aaccagactg cattgcctta tgtatcgttg tcgcaggcac atggccttca atgatcaaat
     2101 taccggcaac cgcggtatga caacttcgca gtcctgcagg aatagcatgc ttttctttca
     2161 gggctgacag cgcctgatca ttcatgacgt gagttcgtac ttcaaacccg tctttttcca
     2221 tcgctttgcc ccacagggaa caacagccac agttttcaga tttgtacata tcaatcactt
     2281 tttcactcgc catcgcgggc agtgaaaggc cgagagccag cgccatcaga accacttttt
     2341 tcatactcac ctctgtatat tatcgattta aaccctgacg tcagggtgaa tcagtaaaga
     2401 aggacaccca caggggtgcc ctttcaggtt caggacacgc tttttttatg tctgcgcagc
     2461 cagattaatt tgtaggcggc aggaataatg aacagggaca gcagcggagc cgtgatcatc
     2521 ccaccaatca tgggtgccgc gatacggctc atgacttctg aacctgcacc ggttccccag
     2581 agtattggca gcagacccgc aatgatcacc gccacggtca tggctttcgg ccggacacgc
     2641 agtacggcac catgatagag ggcttcatca aggccttccg gtgtgaacgt ctctttacgg
     2701 gacaattccg ggtgcgcttc aatggcatga cgcagataca tcagcatgac cacgccaaac
     2761 tctgctgcca ccccggccag agcgataaac cccgttccgg ttgcgacaga catatggaag
     2821 ccctgccagt acaggaacca tatcccgcca accagggcga acggcaggct catcaggatc
     2881 agcagggctt catcaacccg gcggaatgcc agatacaaca ggatgaagat gatcatcacc
     2941 gtcatcggca ccatcagctt cagtttcttg ttggcatgct caagcagttc aaactgtccg
     3001 gagaatgaca cgctggtacc cggtctcagt ttcactttct gactgatcgc cgttttaatg
     3061 tcattaacca ccgacaccat atccctgccg cgcgcgtcaa tgtaaatcca gctggctgga
     3121 cgggcatttt ccgttttcag catagtcggc ccggaaacga ccttaatatc cgcaacatcg
     3181 cccagcgtga tctgctgctt catcggggtc aggatcggca tctgtttcag ggcctgcggg
     3241 ctgttccggt aatcctgcgg atagcggata ttaatcgggt accgggccac gccttcaacc
     3301 gtttccccta ccgtcgcgcc gccgatggct gatgagatga acagctgcac atcgcccacc
     3361 gtcattccgt agcgggaggc tttttcccgg ttgatatcca catcaatgta gcgcccgcct
     3421 tccagtcgct ctgcgagagc agacactacg ccgggtacgg ttttggcgac cgcttcgata
     3481 ctctgcgccg ttgcatcgat atcggacaga accgttccgg acaccttgat acctatcggg
     3541 cttttgatcc cggttgagag catatcaata cggttacgga ttggcggcac ccagaggttt
     3601 gccagccccg gtaaacggac ggtcctgtcg agttcttcaa taatcttgtc aatcgtcatg
     3661 cctggacgcc actgatcctc aggtttgagc tggatcgtgg tttcaaccat ttcgagcggt
     3721 gcggaatccg tggcggtctc tgctttaccg gtcttgccaa atacagaggc cacttcagga
     3781 acgcttttga ttaacttgtc cgtcgtctgc aggagcgctg cagcttcagc cggagagaca
     3841 cccggcaggg tcgacggcat atacaacagg tcgccctcgt taatcttcgg cagaaactca
     3901 ccgcccacct ggctcagcgg ccagataacg gtgaaaatgg acaaggccgc aaccagcagg
     3961 gttgtttttg gccagtggag aacccgcaac agcaatggat gatacgcttt gatcagtacc
     4021 cggttcaggg ggttactggt ctctgcagga attttccccc ggatccagaa tcccatcaga
     4081 atcggaatga cgatgatggc cagcgcggcc gcgcccgcca tggagtacgt tttcgtgaat
     4141 gccagcgggc caaacagacg tccttcctga ccttccaggg taaagatagg aataaaggac
     4201 agggtgatga tcagcaggct gataaacagt gcgggtccca cttcaacgga ggcatcggta
     4261 atcaccttcc agcgggtggc gttgtcaatc tgctcacccg gatgctgatg atcccactcc
     4321 tcaagccgct tatgcgcatt ctcaatcatc acaatggcgg catccaccat cgcaccgacg
     4381 gcaatcgcta tccctcccag cgacatgata ttggcgttca gtccctggaa gtgcatgacg
     4441 ataaaggcga tacacagacc aagcggcaga gagataatcg ccaccagggc agaacgtacg
     4501 tgccacagga acagggcaca gacgatggcc accacgataa actcttccag aagtttggaa
     4561 ctgaggttat caatcgcccg gtcgatgagc tggctgcgat cgtaggtggt cacgatttca
     4621 acgccttccg gcaggctggc cttcagcgtc tcaagtttat ccctcactgc cgtgataacg
     4681 tcgcgtgcat ttttacccga ccgcaggatc accacgccgc cagcgacttc gccctggccg
     4741 ttcagctcgg caataccacg ccgcatttca ggcccggtct gcacgcgggc aacatcccgc
     4801 agataaaccg gcacgccgtt ctcgcctgtt ttcaggacga tgttattaaa atcatcaatg
     4861 ctctgaagat aaccgctggc acggaccata tactcagctt cggccatttc aacggatgag
     4921 ccaccggcct cctggttaga cgattcaagg gcctgtttca cttcgggcag gctgatacca
     4981 tactgagaca attttaccgg attgacctga atctggtact gtttcaccac gccgccaacc
     5041 gaagcgacct cagccacgtt cgggatggtt ttcagctcaa atttcaggaa ccagtcctgc
     5101 agagaacgca gttctgaaag gtcgtgtttt ccgctgcgat cgacaagggc atattcaaat
     5161 atccagccca cccccgtggc gtccggaccg atttcagaac tcacaccggc gggcagtttt
     5221 ccctgtacct ggttcaggta ctccagcacg cgggaacggg cccagtacag atcggtgccg
     5281 tcttcaaaaa tgacatacac atacgaatca ccgaactgtg aaaaaccacg cacggttttt
     5341 gcgccaggca cggacagcat ggtggtggta agtggatagg tgacctggtt ttctacaatc
     5401 tgcggggcct ggccgggata gctggttttg ataatgacct gcacatctga caggtcaggc
     5461 aaggcatcga caggcgtgtt aataatcgtc catgtgcccc agatgctgag aaacagggcc
     5521 cccatcatga ccaggaaacg gttggcgaca gagcgccgga taatccattc aatcatcgtc
     5581 gtctcctcag tgccctgaat gcatatttac aggctggtca gacattgctg gcatactgct
     5641 ttctgttttt tcagggtggc gcatacgttc cagtgcgccc gtaatattgg cttcagagtc
     5701 aatgaggaac aggccactga ccaccacggt atcgccttca ttcaggccgg agccgatgcc
     5761 ggactgttgc tgtgactcat gcagaacgtg gatctgtttc ggcacaaact tgccttcatc
     5821 atcaacagta atcacgcgct gttctttgcc ggtatcgata acggcctggc ttggtatcag
     5881 cagcatctcc tggcttctgg tattcagttt cagataggca ttcatgcccg gcttgagaaa
     5941 ctcatcctta ttagaaacct ggagacggac ctgaagcgta cgggttgtct gatccacgct
     6001 gggaagaata ttccattttt cgacatggaa tgttttatcc ggataagccg gtaccgaaat
     6061 ttcaaactgc gacgtatctt tcagcagata ggcgatagat tctggcactg cagcgctgat
     6121 ccagaccggg tccattccct gaatctgagc caccacctta tctttcgaaa tattcatgcc
     6181 ggtgcgcagg tcaaatgcag taatgacacc atcaataggt gctttaatgg taaaacgggt
     6241 ctgaatgctc cgggtagaac gcagtctctg aatatcctct tccggcatac ctgccaggcg
     6301 aagccgttcc agaacgcctt taatttgcgt ggaagtacca ccggtgctgg ataacagaag
     6361 gaattcactc tgtgcttcca cccagtccgg aatggtaata tcgataagtg gcgtcccctt
     6421 cttcacatga tcgccaatcg tcatggggta gactttttcg acaaacccgt cagaacgcgc
     6481 ctgcacaata acaaactgat actcgttata gctgacatta gccgggattg tctgagaata
     6541 attcagcatt cctcgcgtga ctttttgcgt ttttaatccc agattctgaa cctgggttgg
     6601 gtcgatacgg atcccgccac tgcttttatc gccactgtca tcagcatatt ttggcaccag
     6661 gtccatatcc ataaagggcg attttccggg tttatcaaat ttggtatccg gtttcattgg
     6721 gtcataccag aaaagcacct ttcgttccgg tgccttttgt tctgtttttt ctgtcttttg
     6781 tgctgagttt acatactgcc aggcagtaac cgatatcagc ccccctgcta tgaggctgct
     6841 gataattatt gcagcatatt ttatctttaa agaagccata caattttccg ctaaaaaatc
     6901 aaaacacctg gcatatgcgc ctgacggtta actcacacat atacctatgt gaatgtacag
     6961 gcgcacagga tgtaatcgct ccggacagcc aatcaggact gcgtcacgtt aatgcttttg
     7021 agtaaggaga tattgccctg ctgaataaac gagaaatcga catggttgcc tgttttcagg
     7081 gcattaatag catcgtctgc atttacaaaa gtaaagcgca tggtcattgc aggccagccc
     7141 accgctggaa ttgcttcatg cgaaatggtg attttcttag tattcatatc aatgtcttta
     7201 acgacaccgg tgcccttgat aacttgctgt accgaagcat cactggcagt attcatatcg
     7261 ccatgctgat gtgtttcagc atgaagaccg gcagaaaaca tgacagagaa ggcaccaaat
     7321 aaaacggctt taagtgaatt acgcatttta atttcctgat taattaaata aatttactct
     7381 acccaaccgc cacccagcgc ggtaaacaga ttaatttcgt taacctgccg ggaataggta
     7441 agatcgagaa tggtttgctg ggtagcgaag agggaacgtt ctgcatccag cacttcgatg
     7501 taactgacag caccacttgc atacaatcct ctggcacgct ggagagttat ctgaagtgaa
     7561 tcaagataac gctgctgtga ctcaagttgc tggctaaggc tgtcgcgcag cgcaagcgtg
     7621 tcggaaacat ccttaaaggc tgactgaatt ttttgttcgt aattaaccac cgactgttgc
     7681 tggcgaattt cagccagctt cagattggct ttattcctgc cagcattaaa tataggaatt
     7741 tcaattttag ggataaaatt ccacattcca cttcctgacg taaacaggct ggacagctcc
     7801 gtactgcttg cggaaagacc actggtcagg gtaattgagg ggaaaaaggc cgctcgcgct
     7861 gcgccaatat tggcatcagc cgctttcagc tgatattccg cttccataat atccggtcgc
     7921 tgcagcaaaa tttgtgaaga cagatttggt ggcaatttta ctggggctat ctccccgcct
     7981 ttcatccctt tttctgacgg aagtgcgcgg tacgttccca gcaccagttg cagggcattg
     8041 tttgcctgag ccagatcgcc ttctcgtttg gctatttcgg cgcgggtact ttcgatttgt
     8101 cctcgcgcct gttcaagtgc cagaacgttc gtactcccgg tcacgagctg ctgctcaacg
     8161 aaagcatagg actgttgata atttttcagc gtttcccgcg caatacgaag ttgttcgtac
     8221 gccagttgct ggctgaaata gctttgtgaa acgttggata ccagcaggat gtgtacggcc
     8281 cgacgggctt cttcgctggc aaagtagttc tggcgatcag catcactcat gttcttaagt
     8341 ttgccgaaaa aatcgagctc atagctgagc tccagtctcg cgtcgtactc ctgtgtggtc
     8401 ggcttgtcac ctttcagacc accgctgtat gttatcccgg atgaggcatt cagctgggga
     8461 taacgatctg catccgtgac gttgaactgg gctcgggcct cttcaacctt cagggcagcc
     8521 attctcaaat cacggttatt agtcagagct tcaccgatca accgggtaac ctggggatcg
     8581 acaaaaaagt tacgccagcc cgtatcctga tagccattta ccgctggcgt caggctgtta
     8641 tgggacagtg aaaactgctg gggtaccggt gctgcgggcc gctgatattc aggcgcaagc
     8701 gacacgcagc ctgccaggat gaatatcgtg ctaatgctga gtaattttaa tttgaacata
     8761 acgcttctcg tccaggcaga cctggtaaat ggttcaaatg aagtccagag tattgatagg
     8821 ggtactttac ctaacgctct ctgtttgccg ggtgacagga taatgacaat gttgtcattt
     8881 ttgctgtaat ccgttgataa cgatcgtggg cgcaatagaa tgacattagc agccagccgg
     8941 ggagcaagat gaaaatattg atcgtcgaag acgaaattaa aacaggtgaa tatctcagca
     9001 aagggcttac agaggcaggg ttcgtagtgg atcacgctga taatggtctt accggatatc
     9061 atctcgccat gacagccgag tatgatttag tcattctgga tatcatgcta cctgatgtga
     9121 acggctggga tatcatccgc atgctgcgca ctgccggaaa gggtatgccg gtcttactgc
     9181 tgacagccct cggcacgatc gaacataggg tcaaaggact ggaactgggt gcggacgatt
     9241 atctggttaa accctttgcg tttgccgaac tgctcgcccg ggtgagaacc cttctgaggc
     9301 ggggaaacac gatgatcacg gaaagccagt ttaaggtggc tgacctctcg attgatctcg
     9361 tatccagaaa agtcagtcgc gccggaaacc gcattgtgct caccagtaaa gagttcagcc
     9421 tgctggaatt cttcattcgc catcagggag aggttcttcc ccgctccctg attgcctctc
     9481 aggtctggga catgaatttt gacagcgaca ctaatgcgat cgatgtcgca gtaaagcgac
     9541 tccgcgctaa aatagacaac gattacgaga caaagctgat ccagacagtc cggggcgtgg
     9601 gctacatgct ggaggtcccg gatgcatagc aaaccttcca gacgcccttt ctcactcgct
     9661 ctgcggctga ccttttttat cagcctgtcc acgatactgg cttttatcgc attcacctgg
     9721 tttatgctgc attctgttga aaatcatttt gccgagcagg atgtcagcga tcttcaacaa
     9781 atcagcacca cactgaaccg tatactgcag tccccggtgg atccggatga taaaaaaata
     9841 agcaaaataa aggaatcaat tgccagctac cgcaacgttg cccttttgct cctcaatccc
     9901 aggggggaag tgctctttag ctcagctcag ggggcggcac tacgcccggc agtgaattca
     9961 gcagatttta gcgagcacag ccgcgcacgg gatgtctttc tctggacggt ggaggatcct
    10021 gcgggaccga tggataccgg gtccgaaatg aagatggaaa catacaggat tatcgcctcc
    10081 tctggtcagg cgatatttca gggcaaacag cagaactatg tcatgctgac tggcctatcc
    10141 attaatttcc atctccatta cctcgatgcg ctgaaaaaga acctgattgc gattgccgtc
    10201 gtgataagcc tgttgattgt tctgatcatt cgaatcgctg tccgtcaggg gcacctgccc
    10261 cttcgtaatg tcagcaatgc cattaaaaac atcacctccg agaatcttga tgcgcgactg
    10321 gaaccgacac gcgttcccat tgagctggag caactggtta tctcgttcaa tcatatgatt
    10381 ggaaagattg aggatgtctt tacccgccag gccaatttct ctgccgatat cgcgcatgag
    10441 atcagaacgc ccatcaccaa tctggtgacg cagactgaaa tcgcactgag tcaggatcga
    10501 acacagaggg aacttgagga tgtcctctat tccagtcttg aagagtataa ccggatgacc
    10561 aaaatggtca gcgatatgct gttcctggca caggcagata ataatcagct gatacctgac
    10621 agggtcatgt ttgacctcag agcggaagtc atgaaagtct tcgagttttt cgaagcctgg
    10681 gccgaagaac gcaatatcac gctcaaattt aacgggatgc cctgcctggt tgagggagat
    10741 ccacaaatgt tcagaagggc gatcaataat ctgttatcca atgccctgcg ttataccccg
    10801 gagggacagg caatcaccgt ctcaataaga gagcaggaga gcttttttga ccttgtgatt
    10861 gaaaatccgg ggaaaccaat ccctgaagag catttatcaa ggttgtttga ccgtttttat
    10921 cgggtggatc cgtccagaca acgaaaagga gaaggcagcg gcatcggcct tgcgattgtg
    10981 aagtcaatcg tggaagcaca tcacggaaga gtgcaggtgg aatcggacgt acactcaacg
    11041 cgttttatct tatccgtgcc cagactggag aaaatgattc cggacaccca gtgctgggaa
    11101 taaagattta aatgacaaag atgtcattag cctgtcatgc agcaaacaga agccattcga
    11161 tataattagt gcaacttatc aggaaggctg gattgcttta tcaatatccg gcgtcagtgg
    11221 acgccaggaa atgaatatcc agtcccctcc cggagaaata aacacttccg aacctgtttc
    11281 agttatggaa ctgaaaacac cggttgtact cccccggaca tcactaatta agaaatggag
    11341 agttatcatg aaaaatatcg tattagcatc cttgctgggc tttggcttaa tttcttcggc
    11401 ctgggccact gaaaccgtga atatccatga gcgggtcaac aatgcacagg cacctgctca
    11461 ccagatgcag tctgctgcgg ctcctgtcgg gatccagggg actgcacctc gtatggccgg
    11521 tatggaccag catgaacagg ccattattgc tcatgaaacc atgacgaacg ggtcggcgga
    11581 tgcgcaccag aaaatggtgg aaagtcatca gaggatgatg ggaagtcaga ccgtttcccc
    11641 taccgggccg tcgaagtcat tagcggcaat gaatgagcat gaaagagctg cagttgccca
    11701 tgaatttatg aataacggtc agtctggccc acatcaggcc atggccgaag cgcatcgtcg
    11761 catgctcagt gcaggctgac ggcgagggaa gtatctgtgc tgtccttatt tccttgatag
    11821 atttttccgt aaccggatat atggcaacat ctttgccccc tacagggggc atttttatca
    11881 gattctgcta cagttaacgg gcatcaacgc taaaaggata ttttttatga aagccagaaa
    11941 cactttattt gcagtattaa tgttatccct gccagcgatt tcagcagaac attcagaaat
    12001 gaaaatgact gatatgtcta cctcggcttc gtcacaggaa tatatggctg gcatgaaaga
    12061 catgcatgac aaaatgatgg ctgccgtaaa tgagtccgat cccgacaagg cttttgcgaa
    12121 aggcatggta gcgcaccatg aaggggcaat agcaatggct gagaccgagc tcaaatacgg
    12181 aaaagatccg aaaatgagaa agctcgcgca ggacatcatt aaagctcaaa aaggtgaaat
    12241 tgagcagatg aataaatggc ttgatagtca aaagtaaaca ttgtagaaat gtcacgacaa
    12301 gcgtgatttt acatcgttcc tgggcgcggg agtcggttaa tctttatata attcaaaccc
    12361 gattaatttc gggtttttac tactctgtac tccggtcctt tgttgaggac cggaatatat
    12421 gcacctgaga tgcttattta atacattatc tcgtcatgga tacaatgtcg ctcaggatct
    12481 caaccagctc gcttctctcc tgcgttccgt catgataggt tgatcttgag atgattcttt
    12541 ctgatgtcat ctgaggccag aatttatcta gtacattatc gggtttcatt cccacagccc
    12601 ggccgattat ttttttagcg tcacaggtgt ccgtaaacgt gacggctaca ccagtaattt
    12661 cctcgataag atccgggcac agatagtttt ctatctcacg tttgcgcgta gcgaaaaata
    12721 ccttaccggc ctcctctact tcttttttac gcttctggat ggacagaacc tgtgcagggt
    12781 ctcccccaat atcggagtcc agaaatacgc accagggaag ccccagatct ttggccagat
    12841 tcaatgtaac ccagtgtttg acgctaccac accctcctat gaggactggc actattttca
    12901 cgtcctccag agaggctggc agcgcacctg actgttttaa tgaactggcc gcatgcctca
    12961 ggaaagtaac atccgacttt ccctctacca gaacaatccc ctgcgccctt tccataccgg
    13021 tctctggcag cacccccagg ctttcagtgg cttccttcag cactgcgtca tccggcattt
    13081 ttactacggg ttcacccgcc tcatttcggg taacataacg tacgccttcg acagggatta
    13141 atccggccag cgccgggaca tgggtggtga cgataatctg acggtgcggc tggcctgcca
    13201 gtgccagtaa cgctttcatc agcatcatct gatagttcgg atgctgtgac gtttcaggct
    13261 cctctatggc ataaatcaca tttctgggcg tccccgcgac attcttttca gcctcagccc
    13321 gaaaaaaatt caacaggata agcctcctta tcccgctgcc gcgcttattg atggggatgc
    13381 cattttcccc gtccagggtg aaattgaagg tccacttggg tttctcctta aatcgtggag
    13441 tcagttcact ggcgagttcg ggggccattt cacgtaattt atccagcgtt ctctgtgcga
    13501 catccaggac gctgtcctga atctcatttt ccagcgctgt aattttgtcc tgcagcgcag
    13561 cctgagcgtc ttttacggcc tgctgtaagg ggtttttagc ttcggaatcc ccgtcgctgc
    13621 tttccctgtc ggctttgaaa atcgcatacg tgggcagcaa atcgaggatc ttaccccata
    13681 atgattttgt gtcggttccg aactctttat cgacatccag cataatttcc gaacaggtat
    13741 agggggctgc ggcttccctg atggcctgac gccataacga tgcagtgcgt ttatccgcca
    13801 catttttttc aacttctttt cccaccgcct tgagctcaga aattttcatg cccagtaaat
    13861 ttctcagggg ctcctcatcc gggtggatgc agcggatgca ggtctgctcc ggttttcctg
    13921 aggtcgttgc tttaaacgtt ttgacgattt caaaattgcc gtcctcattc agcagatgtt
    13981 ctgaggcgag cgtggtctgc accctgtcgt cgatcatgat aaaatcgggc aggtcatcaa
    14041 attcacaggc tatttcaaaa cggccgtccc cttctcttag gctgaaacag ttcatgtcat
    14101 tcttgtcagc cttcattcct tctgtttcaa aaaaaatagc cagcgcttcc agaagcgttg
    14161 atttcccaaa gtcattacga ccgacaatac ccgtcatgct ttcatcaata atgatttcag
    14221 tagagtttct gtagcctctg aagtttttca gttttaattt tctgagccgc atatgaagtc
    14281 ctgtgtgtaa gaaagcgttt tgctgcaacc tgaatacaaa atatattttc aaatgcctgc
    14341 caacaagcag ggataaccgt tattcagaat atcttaacga catttcggga ttatgatctg
    14401 gtcgcgcttt cagaacttta ctgcccctca aaacaccgga aactaacttg tggttggtta
    14461 catggcaaag ttaaaactcc agaatcacgg gtaatgattt caatgcttat tat
//
LOCUS       DAZFZC010000113        14504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFZC010000113 DAZFZC010000000
VERSION     DAZFZC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.114"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            267..5687
                     /locus_tag="ACHKXP_004122"
                     /pseudo
     CDS             267..5687
                     /locus_tag="ACHKXP_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5910..6176
                     /gene="hipB"
                     /locus_tag="ACHKXP_004123"
     CDS             5910..6176
                     /gene="hipB"
                     /locus_tag="ACHKXP_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO7649016.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPESTEQQNLEW"
     gene            6176..7498
                     /gene="hipA"
                     /locus_tag="ACHKXP_004124"
     CDS             6176..7498
                     /gene="hipA"
                     /locus_tag="ACHKXP_004124"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO7649017.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            7810..8018
                     /locus_tag="ACHKXP_004125"
                     /pseudo
     CDS             7810..8018
                     /locus_tag="ACHKXP_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8273..9499
                     /gene="ydeT"
                     /locus_tag="ACHKXP_004126"
                     /pseudo
     CDS             8273..9499
                     /gene="ydeT"
                     /locus_tag="ACHKXP_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            9513..10043
                     /gene="ydeS"
                     /locus_tag="ACHKXP_004127"
     CDS             9513..10043
                     /gene="ydeS"
                     /locus_tag="ACHKXP_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7649018.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            10056..10559
                     /gene="ydeR"
                     /locus_tag="ACHKXP_004128"
     CDS             10056..10559
                     /gene="ydeR"
                     /locus_tag="ACHKXP_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7649019.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTDSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            10618..11532
                     /gene="ydeQ"
                     /locus_tag="ACHKXP_004129"
     CDS             10618..11532
                     /gene="ydeQ"
                     /locus_tag="ACHKXP_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7649020.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            11866..14145
                     /gene="ydeP"
                     /locus_tag="ACHKXP_004130"
     CDS             11866..14145
                     /gene="ydeP"
                     /locus_tag="ACHKXP_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO7649021.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMITLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            14393..>14504
                     /gene="safA"
                     /locus_tag="ACHKXP_004131"
     CDS             14393..>14504
                     /gene="safA"
                     /locus_tag="ACHKXP_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO7649022.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIF"
BASE COUNT         4032 a         3128 c         3687 g         3657 t
ORIGIN      
        1 gttaaatatg atgatgataa ttttcttatt gaaagaaaca gttttcttat taagcaacaa
       61 taactcatag ttcattttta aataaaataa gaactatgca atttttaatc tatcaattcg
      121 aaagcattag caaattagcc aaattgacac cttcccacct ccaactaaca ctcaatacag
      181 acctctgaca atttaataag tttcattttt aatgtctgac gactcatctc tttcgtggct
      241 gtattgtttg tatatggagc gtttatatga atagaatcta tcgcgtgata tggaattgca
      301 ctctacaggt atttcaggcc tgctcggaat taactcgcag ggcaggtaaa acatcgacgg
      361 ttaatttgcg taaatcctct ggactgacaa cgaaattcag tagattgacg ctgggtgttt
      421 tgctggcact aagcggttca gcgtctggtg caagtctgga agttgataat gatcagatta
      481 ccaatattga tactgatgtt gcttatgatg cctacctggt tggctggtat ggcactggag
      541 tgcttaatat tttggctggc ggtaatgcct ccttaaccac tattactacc agcgtcattg
      601 gcgctaatga ggactcagag ggcaccgtta atgttttggg tggcacctgg cgattgtatg
      661 atagcggaaa taatgcaagg cctttaaatg tgggtcaatc cggaacgggg acgctgaata
      721 ttaaacagaa gggtcacgtc gatggaggct atttaagatt aggttcttcg acaggaggcg
      781 tcgggacggt caatgttgag ggagaggact ctgttctgac gaccgaatta ttcgaaatag
      841 gtagctatgg cacaggttca ttaaatatta cggataaggg ttacgtcacg agttcaatcg
      901 tcgccatttt aggctatcag gcgggcagta atggccaggt tgtcgttgaa aagggtggcg
      961 agtggctaat aaaaaataat gattcctcaa ttgaatttca aattggtaat caaggaactg
     1021 gggaggcgac tattcgcgag ggggggctgg ttacggctga aaatacgatt atcggtggca
     1081 atgccaccgg tatcggaacc ctgaatgtgc aggatcaaga ctctgtcatc acggtacgca
     1141 gactctataa tgaatatttc ggtaatggca cagtcaatat ttccaataat ggactgatta
     1201 ataacaaaga atattcattg gtgggcgttc aggacggttc ccacggtgtc gtcaacgtga
     1261 ccgataaagg gcattggaat ttcctcggaa cgggcgaagc tttccgctat atctatatcg
     1321 gtgatgctgg cgacggtgaa cttaatgtct cgagtgaagg caaagtagat tcgggaatta
     1381 tcactgcggg gatgaaagaa acaggcacag gcaacattac tgttaaggat aagaactccg
     1441 ttatcactaa tctcggaact aatcttggtt atgacggcca cggcgaaatg aatatcagta
     1501 atcaggggct tgttgtcagc aacggaggaa gttcactcgg ttatggagaa accggcgtcg
     1561 ggaatgtcag catcaccacg gggggaatgt gggaggtcaa taagaatgtc tataccacca
     1621 ttggtgttgc gggcgtcgga aacctcaata ttagcgatgg cggtaagttc gtatcgcaaa
     1681 atattacttt tttgggcgat aaagcaagcg gtatcggcac actgaacctg atggatgcga
     1741 catcatcgtt cgatactgtg ggtatcaatg tcggtaattt tggtagcggt atcgtaaatg
     1801 tcagtaatgg tgccaccctt aattcaacgg gctatggatt tatcggagga aatgcctccg
     1861 gtaaggggat agttaatatt tcaacggaca gtctctggaa tttaaagaca tcatctacta
     1921 acgcacaatt gctacaggtc ggtgtattag gcacgggtga actgaatatt accaccggag
     1981 gtatagttaa agcgcgtgat acacagatag ctctcaatga caaaagtaag ggcgacgtga
     2041 gggtggatgg gcagaactct cttcttgaaa cattcaatat gtacgtaggg acatctggta
     2101 cgggtacgtt aaccctgacg aataacggta cgctgaatgt cgaaggtgga gaagtttact
     2161 taggtgtttt tgagcctgct gtaggaacgc taaacattgg tgctgctcac ggtgaggcgg
     2221 cggcagatgc cggatttatc accaatgcga cgaaagtgga gtttggtctt ggcgaaggcg
     2281 tttttgtctt taatcatacc aataacagtg atgccggcta ccaggtcgat atgctgatta
     2341 caggtgacga taaagacgga aaagtgatcc atgatgcagg ccatacggtg ttcaatgcag
     2401 ggaatactta tagcggtaaa acgctggtca atgacggcct cctgaccatt gcgtctcata
     2461 cggcagatgg ggtaacgggc atggggtcga gtgaagtaac cattgcaaac cccggtacgc
     2521 tcgacattct cgcatcaacg aacagtgcag gagattacac gctgaccaat gcgctcaaag
     2581 gcgatgactt gatgcgagtg cagctgtcat cctccgacaa gatgtttggc tttacccatg
     2641 caacagggac tgaattcgcc ggtgttgccc aactgaaaga cagtaccttc actctggaac
     2701 gcgacaacac cgctgcgctt actcacgcga tgttgcagtc tgacagtgaa aataccacat
     2761 cggtaaacgt gggagagcaa tccattggtg gactggccat gaatggcggt acgctcattt
     2821 tcgatacgga tattcctgct gcgacgctcg cggagggata tatcagcgtc gatacgctgg
     2881 ttgtcggcgc gagtgactac acctggaaag gccgtaacta tcaggtaaac gggacgggcg
     2941 acgtgcttat cgacgtgcct aaaccgtgga atgatcccat ggcgaataac cctctgacga
     3001 cgctcaattt gctggaacac gacgatagcc atgtcggcgt tcaactggtg aaggcgcaaa
     3061 cggttattgg gtcgggtggc tcattaacgt tacgtgattt acagggcgac gaggtggaag
     3121 cggacaaaac gttacacatt gcgcaaaacg gaacggtggt cgccgagggt gattatggat
     3181 tccgcctcac gaccgcacca ggtaatggtt tgtacgttaa ctatgggctg aaagcgctga
     3241 acatccatgg tgggcaaaag ctgacgttag ccgaacatgg cggagcctat ggcgcaacgg
     3301 ccgatatgtc ggcaaaaatc ggtggtgaag gggatctggc aatcaatacg gtgcgacagg
     3361 tttcgctttc caacggtcag aacgactatc agggggcaac ctacgttcag atggggacat
     3421 tacgtaccga tgcggatggt gcacttggca acacccggga actgaacatc agcaacgcgg
     3481 ccatcgtcga tcttaatgga tcgacgcaga cggtagagac attcaccggg cagatgggtt
     3541 cgactgtttt gttcaaagag ggggcgctga cggtaaataa aggtgggatc agtcagggtg
     3601 aactgacagg tggcggaaac ctgaatgtta cagggggaac gctggctatc gaggggctta
     3661 atgcacgcta caatgcgtta accagcatta gcccaaatgc ggaagtcagc ctcgataata
     3721 ctcaggggtt aggcagagga aatattgcca atgacggtct gttaacgcta aaaaacgtga
     3781 ctggcgaact gcgtaatagc ataagcggga agggtatcgt gagcgcaacc gccaggacag
     3841 atgtagagtt ggatggcgat aatagccgct ttgtggggca attcaacatt gatacaggca
     3901 gcgcgctcag cgtcaacgag cagaaaaacc tgggtgatgc ttccgttatc aataatggcc
     3961 tgctcaccat ctccactgag cgtagctggg cgatgacgca cagtatcagc ggtagcggtg
     4021 atgtgacaaa actgggtacc gggatcctga ctcttaacaa cgattccgcg gcgtatcagg
     4081 gtacgacgga tatcgtgggg gggaaattgc tttcggttcc gactctgcca ttaatatggc
     4141 aagtcaacac attaatatcc ataacagcgg tgtgatgtcg ggaaatgtca ccactgcagg
     4201 tgatatgaac gttatgcctg gggggggcac tgcgtgtcgc taaaaccact atcggcggca
     4261 acctggagaa tggcggcacg gttcaaatga atagcgaagg ggggaaaccg gggaatgtac
     4321 tgaccgttaa cggcaactat accggaaaca atggcctgat gacgttcaac gcgacgctgg
     4381 gcggcgataa ttcgcccacc gataagatga acgtgaaagg cgatacccaa gggaacactc
     4441 gcgttcgggt tgataacatt ggcggcgtcg gtgcacaaac ggtcaacggt attgaactca
     4501 ttgaggttgg cggtaattct gcaggtaact tcgcgctgac caccggaact gtcgaagctg
     4561 gggcttacgt ctacacgctg gctaaaggga aggggaatga cgagaaaaac tggtatctga
     4621 ccagtaaatg ggacggcgta acgccagcgg atacacccga tcccatcaat aatccccctg
     4681 ttgtggatcc ggaaagccca tcagtttatc gcccggaggc cggaagctat atcagcaaca
     4741 ttgccgcagc caactcgctg tttagccatc gtttacacga ccgtctgggt gagccgcagt
     4801 atacagattc actgcattct cagggggcgg caagcagtat gtggatgcgt catgtcggag
     4861 ggcacgaacg ttcaagggcc ggtgacggtc agctaaatac tcaggctaac cgctatgtat
     4921 tgcagctagg cggcgatttg gcgcagtgga gtagcaacgc gcaggatcgc tggcatcttg
     4981 gcgtgatggc aggctacgcc aatcagcaca gtaatactca gagtaatcgt gtgggttata
     5041 aatcggatgg gcgcatcagc ggttacagcg ctgggctgta cgcgacctgg tatcagaacg
     5101 atgcgaataa gaccggcgct tatgttgaca gctgggcgct gtataactgg tttgataaca
     5161 gcgtcagttc cgataaccgt tctgctgacg actatgattc tcgcggtgtg acggcctctg
     5221 ttgagggtgg gtataccttt gaagcgggaa catttagcgg cagcgaaggg acgctgaata
     5281 cctggtacgt ccagccacag gcgcaaatca cctggatggg tgtgaaagat tccgaccata
     5341 cccggaaaga cggaacgcgc attgaaacgg aaggcgacgg aaatgtgcaa acgcgacttg
     5401 gggtgaaaac ctacctgaac agccatcacc agcgtgacga tggtaaacag cgtgagttcc
     5461 agccttacat tgaagcgaac tggatcaaca atagcaaagt ctacgccgtg aagatgaatg
     5521 gtcaaaccgt aggccgtgaa ggtgcgcgta atctcggtga agtacgtacc ggggttgagg
     5581 cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg tgtgcaacta ggtgataaag
     5641 gctatagcga tactcagggc atgctgggag tgaaatatag ctggtaaacc gtataagccg
     5701 catgtcgaga tggcatgcgg cttaatattg ccgacttcaa acggcgcatc aacgccttat
     5761 ttaaatcctc ctttttatcc gcgatcgcgg atatcgcagc gtttatcccg tagagcggat
     5821 aagatgtgtt tccagattga cttatcctca ctaaaggata aaacttataa tatcccctta
     5881 agcggataaa cttgctgtgg acgtatgaca tgatgagctt tcagaagatc tatagcccaa
     5941 cgcaattggc gaatgcaatg aaactggttc gccagcaaaa tggctggacg cagagcgagc
     6001 tggcgaaaaa aattggtatt aagcaggcga cgatttccaa tttcgaaaac aaccctgaca
     6061 ataccacgct cacgacattt tttaagattt tacagtcgct tgaactctca atgacgctat
     6121 gcgacgcgaa aaatgcctcg ccagaatcaa cagaacagca aaatctggag tggtaatgcc
     6181 taaacttgtc acttggatga acaaccagcg ggtaggcgag ttaacgaagt tagccaacgg
     6241 cgcgcacacc tttaagtatg caccggagtg gttagcaagc cgttatgcca gaccgttgtc
     6301 actttcgctg ccattgcaga gggggaatat cacctctgat gccgtattta acttcttcga
     6361 taacctgtta cccgatagcc cgattgtacg tgaccggatc gttaaacgtt atcatgccaa
     6421 atccagacaa ccgtttgatt tattgtcaga aatagggcga gacagcgttg gtgccgtgac
     6481 gttaataccc gaagacgaaa ccgtaacgca tccgataatg gcatgggaaa agcttactga
     6541 agccagactt gaagaagtat taacggctta taaagcagat atcccgctag gcatgattag
     6601 agaagaaaat gactttcgca tctcggttgc tggcgcacag gagaagacag cactgctcag
     6661 aataggcaat gactggtgca ttccgaaagg aataacgccg acgacgcaca tcattaaatt
     6721 accgattggc gaaatcaggc agcccaatgc gacgctcgat ctcagccaaa gcgttgataa
     6781 tgagtattac tgtctgctgc tggcgaaaga acttgggttg aatgttccgg acgcagaaat
     6841 cattaaagcg ggaaatgtgc gcgcgttagc ggtcgaacgt tttgacaggc gttggaatgc
     6901 tgagcgaacg gttttacttc gcttgccaca ggaggatatg tgtcagacat tcggtttacc
     6961 ttcatcggtg aaatatgaat cagatggagg cccaggcatc gcgcggatca tggctttttt
     7021 gatggggtcc agcgaggcgc tgaaagatcg ctatgatttt atgaaattcc aggtcttcca
     7081 gtggttgatt ggcgcaacgg acggtcatgc aaaaaacttc tccgtattta ttcaggctgg
     7141 cggcagttat cgactcacgc cattttacga catcatttca gcatttccgg tccttggcgg
     7201 tacgggaata cacatcagcg atctcaaact ggcaatgggg cttaacgcat ccaaaggcaa
     7261 aaaaacggca atcgataaaa tttatccgcg acattttttg gcgacagcaa aggtgctgag
     7321 attcccggaa gtgcagatgc atgaaatcct gagtgacttt gccagaatga ttccagcagc
     7381 actggataac gtgaagactt cattaccgac agattttccg gagaacgtgg tgacggcagt
     7441 tgaaagcaat gtgttgaggt tgcatggacg gttaagccga gaatacggta gtaagtgaga
     7501 tatggggttt ggtcattgtt aatgagcatg acaatcatga ccgccaacga ctggattctt
     7561 ccttggttta tattggtgcg cataatgtat attatgttaa atcatgtctg agtgatgcga
     7621 agtgcttttc tggtagtcgt tattcgttca gggttcaaga acttaagcct ctcctctttc
     7681 ataattggcc tgtgcatgga gaaatagcct ggttattccc tgccccatgc taagagcgca
     7741 gtgtctgtcg catagacact gcatacgaaa acaccagagg gtgctgttac ttatttaagg
     7801 acggatcaag cgggaatgag tccactccgg tatcagaaat ggctacgtct caatgaagtc
     7861 aggcgaccga tgctgaatga acattacgat gtcaccacag cagcctatgc tgtcggttac
     7921 gaaagctatc ccatttcagt cgggaatatt cgcggatgtt tggagagtca cccaagagag
     7981 atattaccag gttaagaaag tctgtcggac agctttaata aaccctgcac ttatctgttt
     8041 agagcaattc ggtgttagtt tcagcaagca aacattaacc atagctaatg atttatagcc
     8101 atattaacca ttggtagctc ttgacctttc ctgacacttc ccttgttcgc aataatattc
     8161 tgtttagtca cggtttataa ataacatgca taatgaaatc gtcatgttat ttagcgacat
     8221 aaaatgtttt tgcaaatact ctcttcaaca agtgcagcaa taaacttgtt gtggaaccaa
     8281 tatccaggtc gcaggatatc gctattctac gcaaggtttt tataacttat ccgacagtgc
     8341 atacagtcga atgagtggtt acaccgtcaa gcctcctacc ggagacagca atgagcagac
     8401 acaatttatt gattatttta atctgttcta cagtaagcgt ggtcaggaac aaataagcat
     8461 ctctcagcag cttggaaatt acggtacgac atttttcagt gccagtcgcc aaagttactg
     8521 gaacacgtca cgcagcgacc agcaaatatc atttggatta aatgtgccgt ttggtgatat
     8581 tacgacttcg ctgaattaca gctattccaa taatatatgg caaaacgatc gggatcattt
     8641 actcgctttt acgcttaatg ttccctttag tcattggatg cgtacagaca gtcagtcggc
     8701 atttcgtaat tcaaatgcca gttacagtat gtcaaacgat ttgaaaggcg gcatgaccaa
     8761 tctatcgggg gtttatggca ctctgctgcc ggataataac ctgaattata gcgttcaggt
     8821 cggtaacacc caagggggta atacatcgtc tggcaccagt ggttacagtt ctcttaatta
     8881 tcgtggagct tacggtaata cgaatgtcgg ttacagtcgg agtggtgaca gcagccagat
     8941 ttattacgga atgagtggtg ggattattgc tcatgctgat ggcatcacct ttggacagcc
     9001 gctgggcgac acaatggttc tggttaaggc tcctggcgct gataatgtca aaatagagaa
     9061 ccagaccgga attcataccg actggcgtgg ctatgcaata ttaccatttg cgacagaata
     9121 tagagaaaat cgtgtcgctc ttaacgcgaa ttcccttgca gataatgttg aactggatga
     9181 aaccgtagtc actgtcatcc caactcacgg tgctattgcc agagcaacat ttaatgcaca
     9241 aatcggcggg aaagtattaa tgacgttgaa gtacggtaat aaaagcgttc cattcggtgc
     9301 aattgtcaca gacggagaga ataaaaatgg cagcattgtc gcggaaaacg gtcaggttta
     9361 tctgactgga cttccacagt cagggaaatt acaggtttca tggggcaaag ataaaaactc
     9421 aaactgtatt gtcgagtaca agcttcctga agtttctcct ggtaccctac tgaaccagca
     9481 gacagcaatc tgtcgctaag gaacaataga ttatgaaata caataacatt attttcctcg
     9541 gtttatgtct ggggttaacc acctattctg ctttatccgc agatagcgtt attaaaatta
     9601 gcgggcgcgt cctcgattat ggctgcacag tctcatcgga ttcgcttaat tttaccgtag
     9661 atctccaaaa aaacagtgcc agacaatttc caacgaccgg tagcacaagt ccagccgtcc
     9721 cttttcagat tacgttaagt gaatgcagca aagggacaac gggggttcgg gttgcattta
     9781 acggtattga ggatgcagaa aataatactt tgttgaaact ggatgaagga agcaatacgg
     9841 cctccggttt gggtatagaa atattggacg caaatatgcg tccggtgaaa ctgaatgatc
     9901 ttcatgccgg gatgcagtgg atcccactgg taccagaaca gaacaatatt ttgccttact
     9961 ccgctcgtct gaagtcaact cagaagtccg tcaatccggg actggtgagg gcttcggcaa
    10021 cctttaccct tgaatttcaa taatcagagt cagatatgaa aagacttcac aagaggttcc
    10081 tgttagctac gttttgcgcg ttattcacag caactctcca ggccgccgat gtcactatca
    10141 ctgttaatgg tcgggtagtc gctaaaccct gcactattca aaccaaagaa gctaacgtta
    10201 atctcgggga tctttatacg cgcaatctgc aacaacctgg ttctgcatct ggttggcaca
    10261 atattacttt gtcattaacc gattgtccgg ttgaaacaag tgcagtgacg gcaatcgtga
    10321 cagattcaac tgacaatacg ggttattaca aaaatgaagg tactgccgaa aatattcaga
    10381 tagagctgag ggatgaccag gatgctgcgt taaaaaatgg cgatagcaaa acggttattg
    10441 ttgatgagat cactcgtaat gcacagtttc cacttaaggc aagagctatc acggtgaatg
    10501 gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt gatctacacc tggcaataac
    10561 gtcgattcga ttttcttagc cttcttttta aatcaatttt taaccttagg atatatcatg
    10621 ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat ggcaggcaaa
    10681 gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg tacaacctct
    10741 gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt agacttgtct
    10801 cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca tataaacctg
    10861 gtacaaggct cagctttcgc aggctcccta cagtcatata aaggatcgct gtactggaat
    10921 aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat tggtgataag
    10981 acaccgatgc cattaccgtt aaaactctat attaccccag ttggggcggc cggtggagtt
    11041 gtcataaaag cgggggaagt cattgcccgt attcatatgt ataaaattgc aacattagga
    11101 agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacaatgt tgttatgcca
    11161 acaggcggct gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga ctttcctgga
    11221 agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc attttatctt
    11281 tcgggtgcca ccactgacag tagccgacaa gtgtttgcta acacagcacc tgatgcaaca
    11341 aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa aaatactggc tacgggagaa
    11401 aatgtctccc tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat
    11461 ggacaaacag gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt
    11521 atctatgaat aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgcg
    11581 gataattatt ttgtgaaggc tattagccta cacctgtaag aaaatccgcg cacaattgaa
    11641 gccagagtct tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg
    11701 cttaacttat cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt
    11761 agtcccctac tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat
    11821 cgctattaca aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt
    11881 gaatcctacc agggtgctgc aggtggttgg ggtgctgtca aatccgtagc gaatgcagta
    11941 cgtaagcaga tggatatacg ccaggatgtt attgccatgt ttgacatgaa taagccagag
    12001 ggctttgact gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt
    12061 tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct
    12121 ttctttgctg agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct
    12181 gcgggacgtc tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta
    12241 agctggcaac aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat
    12301 caggttgaat tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt
    12361 tttgcccgtg aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg
    12421 acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac
    12481 tttgagaagt gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc
    12541 atgctgactt cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct
    12601 ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg
    12661 aactctgaga ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg
    12721 ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt
    12781 cggccctcat tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta
    12841 cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag
    12901 acacaaatcg ccgaactggc tgacgcatat gccgctgccg aacgcaccat tatctgttac
    12961 ggaatgggga tcactcagca cgaacatggt acccagaacg tacagcaact ggtcaatttg
    13021 ctgttgatga aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgtggtcac
    13081 tctaatgtac agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg
    13141 gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt
    13201 gccagcatgc aagcaatatg tacggggcag gctcgagcat tgatctgcat ggggggcaac
    13261 tttgcactgg caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg
    13321 gcggtacacg tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat
    13381 attctgccgg ttctggggcg tagcgagatt gatatgcaaa aaagcggtgc gcaggcggta
    13441 accgttgagg attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt
    13501 gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccag
    13561 agcgtggtag cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa
    13621 gctgtgctgc cagagttcgc cgactataac cagcgcatcc gtcatcccgg tggttttcac
    13681 ctgataaatg cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt
    13741 accagcaaag ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg
    13801 acagtacgca gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga
    13861 ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt
    13921 gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca
    13981 cgccgcatgg atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc
    14041 tattttccag aatcgaatca catgataaca cttgataacc acgatccatt aagtggcatt
    14101 cctggctata aaagtattcc ggttgaatta gaaccatcaa attaatgcct cttctcattt
    14161 cttctgctgt catccgcaca gcagaagaat tcctcattga ctattatcgc gcaatttgct
    14221 cacatggatt aaattaaact acatactata agatataaac ttctgcctac agctgtaaga
    14281 aactccgctc agtactgaag caccagtcct atttcctctt ttctccagcc tgttatatta
    14341 agcatactga ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgcatgc
    14401 gaccacagtg aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat
    14461 tgtggttgtc ttattactga cacttacgtt gatagccatt tttt
//
LOCUS       DAZFZC010000114        14392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFZC010000114 DAZFZC010000000
VERSION     DAZFZC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.115"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(175..843)
                     /gene="artM"
                     /locus_tag="ACHKXP_004132"
     CDS             complement(175..843)
                     /gene="artM"
                     /locus_tag="ACHKXP_004132"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO7649023.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(843..1559)
                     /gene="artQ"
                     /locus_tag="ACHKXP_004133"
     CDS             complement(843..1559)
                     /gene="artQ"
                     /locus_tag="ACHKXP_004133"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO7649024.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1566..2297)
                     /gene="artI"
                     /locus_tag="ACHKXP_004134"
     CDS             complement(1566..2297)
                     /gene="artI"
                     /locus_tag="ACHKXP_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO7649025.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2315..3043)
                     /gene="artP"
                     /locus_tag="ACHKXP_004135"
     CDS             complement(2315..3043)
                     /gene="artP"
                     /locus_tag="ACHKXP_004135"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO7649026.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDNAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3261..3776)
                     /gene="ybjP"
                     /locus_tag="ACHKXP_004136"
     CDS             complement(3261..3776)
                     /gene="ybjP"
                     /locus_tag="ACHKXP_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7649027.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3902..4225
                     /gene="ybjQ"
                     /locus_tag="ACHKXP_004137"
     CDS             3902..4225
                     /gene="ybjQ"
                     /locus_tag="ACHKXP_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO7649028.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4222..5052
                     /gene="amiD"
                     /locus_tag="ACHKXP_004138"
     CDS             4222..5052
                     /gene="amiD"
                     /locus_tag="ACHKXP_004138"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO7649029.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVQEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTTSL
                     LELLARYGYDVKPDMIPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5049..6062)
                     /gene="ybjS"
                     /locus_tag="ACHKXP_004139"
     CDS             complement(5049..6062)
                     /gene="ybjS"
                     /locus_tag="ACHKXP_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7649030.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6161..7591)
                     /gene="ybjT"
                     /locus_tag="ACHKXP_004140"
     CDS             complement(6161..7591)
                     /gene="ybjT"
                     /locus_tag="ACHKXP_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO7649031.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSRQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7602..8603)
                     /gene="ltaE"
                     /locus_tag="ACHKXP_004141"
     CDS             complement(7602..8603)
                     /gene="ltaE"
                     /locus_tag="ACHKXP_004141"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO7649032.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8640..10358)
                     /gene="poxB"
                     /locus_tag="ACHKXP_004142"
     CDS             complement(8640..10358)
                     /gene="poxB"
                     /locus_tag="ACHKXP_004142"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO7649033.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10491..11459)
                     /gene="hcr"
                     /locus_tag="ACHKXP_004143"
     CDS             complement(10491..11459)
                     /gene="hcr"
                     /locus_tag="ACHKXP_004143"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO7649034.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11471..13123)
                     /gene="hcp"
                     /locus_tag="ACHKXP_004144"
                     /gene_synonym="priS"
     CDS             complement(11471..13123)
                     /gene="hcp"
                     /locus_tag="ACHKXP_004144"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO7649035.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13267..14166)
                     /gene="lysO"
                     /locus_tag="ACHKXP_004145"
     CDS             complement(13267..14166)
                     /gene="lysO"
                     /locus_tag="ACHKXP_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO7649036.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3477 a         3843 c         3808 g         3264 t
ORIGIN      
        1 aaaaagacgg acaacttagt gggttgtccg tcttcattat aagaatttat gcactatgta
       61 ggccggataa ggcgtccccg ccgcatccgg cacaggcacc gtgctgatgt ctgatgcgac
      121 gctggcgcgt cttatcagac ctacaaaacc ccccggcgaa tgtacgcagc cacattaatt
      181 tcgccgttcg aatgccagcg ctttgcgctc gatcagacgc atcatcagcg tcagcaggcc
      241 gttaacgacc aggtaaataa tccctgccgc accgaacacc attacatcgt aggtgcgtcc
      301 gtacaacaac tggctgtatc ccatcacttc catcagcgta atggtgtatg ccagagaggt
      361 acttttgaat accagcacca cttcgttgga ataagaagag agcgagcgtt taaaggcata
      421 cggcagcagg atcgccagcg tatctttttt gctcattccc agggcgctac aggactgcca
      481 ctgaccttcc gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat acgccgcact
      541 attcagcgac aacgcaatca gcgcacataa ccacggttct gacaacaaat gccacagtgc
      601 cggatactcc tgcaaagtcg gaaactggcc cggcccgtaa taaatcagga agatctgcac
      661 cagcagcggc gtaccggtaa acagcgtgat ataaccccgc accagccaca ccagcaccgg
      721 cgttttcagc gtcaggatga tggtaaaaat caatgccaga atcagtgcca caatcagcga
      781 ggcaacggtt agcgtcaggc tggtgtgtag ccctttcatc agttcgggta aatactcaaa
      841 cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt gagaatgtac
      901 tgactgagca gggtgatcac caggtaaatc gccgccgcca caatgtacca ggtaaatggt
      961 tcctgggtac gagtagcgat gctttttgtt tgcagcatta aatcattcac actaatcaaa
     1021 ctgaccagcg cggtatcttt cagcagcacc agccactggt taccgaggcc aggcagcgca
     1081 tgacgccaca tctgcggcat caccagacgg aaaaagatag ccgatttcga cagccccagc
     1141 gcctgaccag actcccactg acccaccggc accgctttca acgcgccccg aagcgtttgc
     1201 gaggcatagg cggcatacag cagtgacaga gcgatgacac cacaaaggaa cgggctaacg
     1261 tcgaagttct caatgtccat ctgcactggg atctgcacga acccaagatt gatagtgaag
     1321 ccatccgaaa gcgtcagcag cagctgcgag gagccaaaat agataaacag caccaccaga
     1381 atttctggca ggccacgcag aatggttacc agcgctgaac ctgcccacgc gacaggacgc
     1441 cattttgccg actcccatac cgcaaagaac atcgccagcg ccagcccgac aatcaatgca
     1501 caaacggcaa ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa ttcattcatc
     1561 aggaattact tctggaacca tttgttgtag atggtttcgt aagtgccatc tttcttcact
     1621 ttttccagcg cagtgttgag tttctgctgc agctcagtgt tgccctgacg taccgcgatg
     1681 ccgaggccag tgccgaagta atctttatcg gtcactttgt cgcccaccgc cgccagtttc
     1741 gggttatctt tcagccactc agtgaccact gcggtgtcac cgaagacgcc gtcgatacgc
     1801 ccgttttgca gatccagttt tgcgttctgg tagctgtcat acggaacggt agtgatttcc
     1861 gggtgcttat ccataatgaa tttctggtgt gtcgtcccgt tctgtacgcc gacttttttg
     1921 cctttcagct gatcaacact ggtgtatttg ccttgctgac ccacaaacag ggcagagttg
     1981 tcatagtacg gggtggtaaa cagcacctgc ttttcacgct ccggagtgat atccatgccc
     2041 gccatcacgg cttctacgcg acggaatttc aggcttggga tcaggctgtc aaacgcctgg
     2101 ttagagaaag tgcaggttgc atcaatctct ttacacagcg cttgtgccag gtcgacgtca
     2161 aaaccaacga tctggttgtt tgcatcaatc gattcaaacg gaggatagga ggcttcggta
     2221 gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa aacctgcaat taacgcggca
     2281 atcagaactt ttttcattgt tgttatcccg aatcttagtg agagagatag tttttaaatg
     2341 cttcggtttg cggctcggta aagcagctcg cgtcgccttg ttctacgata tgaccatttt
     2401 ccatatacac cactcggctg gcggttttac gcgccacttc aacttcgtgg gtgacgatca
     2461 cctgggtaat attcgtttct gccagctcac gaatgatgct gacgatttgt gccgtaattt
     2521 ccgggtccag tgcggcggtc ggttcatcga acagcagtac ctgcggttcc atcatcaacg
     2581 cacgggcaat agcaacacgc tgctgctgac caccagaaag atgcagcggg taacgatcgc
     2641 tataaggttt gagacgcaga cgttccagca gtttttctgc acgggccagc gcctgatctt
     2701 tactcaaccc cagtacacgg cagggcgctt caatcaggtt ttgctgcacg gtcagatgcg
     2761 gccacaggtt gtattgctga aacaccatgc caacgttacg acgcaaatcg cgaatcgcat
     2821 tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc gtaccggagc
     2881 gcggcatctc aagcagattg agtacacgca gcagcgagct tttacccgcg ccgctggggc
     2941 caagtaacac cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac agcgcctgat
     3001 gcgcgccgta gaagcaatta atgccgttta attgaatact cattgacact cgtatactgg
     3061 cagtctgata gctattgagg tcgaagatag tacctttgac agaataatta tgcaatattt
     3121 ctgctttaaa agttaaaagc aaagcgcatt attcaataaa catagcacaa aataacgggg
     3181 gcggtggtcg gcgagcataa atgtcggcat tcctcacgaa atgccggaca atttacgggg
     3241 tttattggtt gatcaaggcg ttagcgattc tcgatggact gacggagcgt acccgccgtg
     3301 gcatgaacgc taccgcctaa gtaacgcaca tcgtcgatga cccaacactg gccttcctga
     3361 atcattaaca cttcatcctg ccagccctgg tcaccctgtt taagatccac gcgcaaagga
     3421 atgttacggg catcacgatt agggatagtc gatgcactgg caacgtgggc gctgtctggc
     3481 aaggtggttc gactggagaa tggatcgttg gtcagtagtt cccgatggtt attatcccgg
     3541 gaggcatcgc taagcagtgt cgccagtttg tcgctcagat aagggcgcaa ggcggtgatg
     3601 tcgttgctgc ggtgcaaaat gcggtagtca taaaattgct gggccacgtt atccgggcct
     3661 ccttcaacgc aaggaccact gcgtgtgccg ttatctttat aagctggagt gactgtggtg
     3721 caggcactga ggagcagtgc gcaggggata agcattgtca atttgctgta gcgcataatg
     3781 atttccttat aagcgatcgc tctgaaagcg ttctacgata ataatgatat cctttcaata
     3841 atagcgtatc agtctgataa tgcttttgag atcgaaggct tagcaaacaa ggagatcgat
     3901 catgcaattt tctacaaccc caactctgga aggccagacc atcgttgaat attgcggtgt
     3961 ggtgaccggc gaagcgattt taggtgccaa tattttccgt gatttctttg ccggtatccg
     4021 cgatatcgtt ggcggacgtt ccggtgcgta tgaaaaagaa ctgcgtaaag cacgggagat
     4081 cgcctttgag gaattaggct cccaggcgcg ggcgctgggg gccgatgccg tcgtcggtat
     4141 tgatatcgac tacgaaacgg tcgggcaaaa cggcagtatg ctgatggtta gcgtcagcgg
     4201 tacggcggtg aaaacgcgtc gatgagaaga gtcttctggc tggtcgctgc cgctctgtta
     4261 ttggcagggt gtgcaggcga aaaaggcatt gtcgagaaag agggatatca gcttgatacc
     4321 cgacgccagg cgcaggcggc gtatccgcgc attaaagtgc tggtgatcca ctacaccgca
     4381 gatgattttg atagctcgct ggcgacactg accgataagc aggtcagctc gcattatctg
     4441 gtccctgcgg taccaccgcg atacaacggt aaaccgcgca tctggcaact ggtgcaggaa
     4501 caagaactgg cctggcatgc ggggattagc gcctggcgcg gggcaacgcg ccttaacgac
     4561 acctctattg gcattgagct ggaaaaccgt ggctggcaaa aatcggccgg agtgaaatat
     4621 tttgccccgt ttgaaccggc acagattcag gcgcttattc cactggcgaa agatattatt
     4681 gcccgttatc acatcaagcc ggaaaacgta gtggcacatg cggatatcgc accgcagcgc
     4741 aaagacgatc cggggccatt atttccctgg cagcaactgg cgcagcaggg gattggtgcc
     4801 tggccggatg cgcagcgggt taacttttac cttgccgggc gcgcgccgca cactcctgta
     4861 gatactacgt cattgctgga gcttttggcg cgctacggtt atgacgttaa acctgatatg
     4921 ataccgcgcg agcagcggcg cgtgattatg gcattccaga tgcatttccg cccgacgtta
     4981 tataacggcg aagcggatgc agaaactcag gcgattgccg aagcattgct ggagaaatac
     5041 gggcaggatt agcgcggcag ttttccgtgg tcgcgtagcc aggcggcagt tttctcgata
     5101 ccttcatcca gggtgatgac cggctgataa cctaactctt cctgcgcacg cgtaatatcc
     5161 agcgtaaagt caaaattcaa cttggagacg ccgtagtggg tcagcggcgg ctcttttgct
     5221 gacttgcggc ctaaacgctc catgctgcgg gcgatcatat ccagcatcgg gtaggggacg
     5281 gaacgaatac gacagtcaat attcaactcg tcgatcagct tctgcacgat gctgcgcagt
     5341 gtgcgatgct cgccgttggt gatgttgtac acacgcccgg aaggtagctt atcgcaggct
     5401 tcctggcttg ccagccacat tgcgtgcacg gcattttcat agtaggtcat atcgaccagc
     5461 gcactgccgc catgcggtaa cagaatactg ccgtagtggt gcatcatatg cgccagacgg
     5521 ggaataaaga ctttatcgtg cggtccgaac agactttgtg ggcgcagaat agtaaagcgc
     5581 gtttgtggat tcgcctgcga aagcatattg atcacttctt cgctggctgc tttgctgcgg
     5641 gcaaactcgt tggcgaagcg gtgagggcga aaatcttctt taatatcgcg atggtggtga
     5701 taatcgaagt acagggaggg ggaagagata tgaataaagt tacgcacacc ccaggcgaca
     5761 gcccattcac ccaggcggcg agtggcgcga acgttagcca gatcgaaagc ctgttgtgtc
     5821 ccccagggtg aggtaaagct ggagcagtgc cacagcgtat caatgcccgc gagcatcact
     5881 ttagcttgtg atgaaaccag ctcggtcaga tccgccggaa caaactctgc gcccattttt
     5941 tccagcaatt tgcccattgc ctcgttgcga ccggtcgctc gcacgctgat gcctttctgg
     6001 cataaaaact ctaccgcgtt tcgacctaag ccgctggtgg cgccggtaac cagtaccttc
     6061 atatcaatcc actgttgttg agaaaataac gtgcgcattc ttccgtgatt tcccccatga
     6121 tgcaatggga aacatgaaag aataacgcag gttttgtcga ttaatctgtg ctttgttctg
     6181 ccagtctggc gatttgtttt gccattccgc gaaaaataaa cagatgcgcg gggatcatca
     6241 ataaccagta aaacagcccc ggcataccgt gcggatgcca gaaagcgcgg acatcgatag
     6301 tacgatagtc gcctttatct tccaggctaa aacacagtcg tcccagcccc ggcgctttca
     6361 tgccaaataa caacgtaagt tgtttttccg gttcaacgac aatcactttc cagctatcca
     6421 ccgcatcgcc agtctgtaaa tattcgcgct ccgggcggcc tttcgccagc ttatgaccga
     6481 tcgcgcggtc catcaacgcc cgtgtctgcc acaaaatatt gccaaagaaa taacgctctt
     6541 taccgccgat ttggttcact acctgccata aagcagcaag gctggcggac gttttaacgg
     6601 taaaccccgc ctgtttggca aaataaccgt actccggtcg ccagcgggca aaggcctgag
     6661 cgtcgtagcc ccagtcgctg gagttgacca gtttttcctc ctctttcaac gtgctacgta
     6721 ccgcgtcatc gaaagcgatc agccgttgtg ggatgagtgc acgtagcgcg gtatcatccg
     6781 ccagcagatc gtgtttcagc ccctgaatca acgccctggc ggtggtgggc ggtacggaag
     6841 taatcacatt gagaaaccac accgaaatcc agcgggtggg gagggggatg gggatcaacc
     6901 agcggcgctt accgctcacc gccataaaat gttcaaactg ttgctgataa ctgagcacct
     6961 ctggtccggc ggcttcgaag atgcggtgtt cgctggccgg atgatctaac aacgccacca
     7021 gatagtgcag caagttttcc agcgcgatgg gcgtggtgcg tgaacgtacc cagcgtggcg
     7081 gcgttaacac tggcaggttg tagaccatat cgcgcatgac ttcgaacgcc gctgaacctg
     7141 cgccaacgat aattccggcc cgaagttcgg tcacaggtac attcgcttca cgaagaatgt
     7201 ccgccgtagc ctgacgagca cgcagatgat ccgactgctc atgtggcggg gcctgcaacg
     7261 aactgagaaa gattaattgc ttaactggta cttcacgtag cgcatcgcgg acgttgagag
     7321 ccacctggcg ctcctgagcg ataaaatcgc cgccttcgcc catgctgtgc accagaaaat
     7381 agacggtatc gatatcctgc aacagggccg gaaggttatc cggccagctg agatcgactt
     7441 tatggcaact gacgtttgcc agttgcagct ttgcaagcct gtcgacatga cgtgccgccg
     7501 ccaggatctg atgcccttgc cggctgagtg tgcgcaccag atgctgacca atgtagccac
     7561 tggcaccgag aactaaaatg cgttgcggca cgtctctctc cttaacgcgc caggaatgcg
     7621 cgccagtggg cggcgacttc cgccagttgt tcgcgcgaga cgtcaagatg cgtcaccagg
     7681 cggacaatcg gcgaggcgtt aatcagcacg tttctcgctt tcatgtattc gcctaacgcg
     7741 gcagcatttt cttccccgac gcgaacaaac agcatattag tgtcctgacg catcacatcc
     7801 gcgcctgctt cacgcagttg ctccgccatc caggcggcgt tgtcgtgatc ttcctgcaac
     7861 cgtgcgacgt tatttttcag ggcatacatc ccggcggcag ccagaatccc ggactgacgc
     7921 atcccgccac cggccatttt ccgccagcgg atggcacgtt taatgtaatc acgattaccg
     7981 acgagtaatg aaccgactgg cgtcccaaga cctttcgaca ggcaaatggt gaacgaatca
     8041 caatattgcg tgatctcttt cagttcgcag ccgtaagcca ccacggcatt aaagatgcgc
     8101 gcaccgtcaa catgcagcgc cagattgcgc tcgcgggtaa attcccatgc ttctttcagg
     8161 tattcacgcg gcagcacttt gccgttgtgg gtgttttcca gactgagtaa tttggtgcgg
     8221 gcgaaatgga tatcgtcggg tttgattttc atcgccactt tatccagcgg tagcgtgccg
     8281 tcggcagccg cgtcgatggg ttgcggctga atactgccca gcaccgctgc gccaccggct
     8341 tcaaacagat agttatgcgc ggcctgaccg acaatatact cttcgccacg ttcgcagtga
     8401 ctgagcagag cgaccaggtt ggcctgagtg ccggtcggca gaaaaatggc ggcttcttta
     8461 ccggaaagct ctgcggcgta gtcctgcaga gcattaacgg tagggtcgtc tccgtaaacg
     8521 tcgtccccaa ccggggcggc catcattgct tcgagcatgg cgcggctcgg tcgggtaacg
     8581 gtatcactgc gtaaatcaat catggcatgt ccttattatg acgggaaatg ccaccctttt
     8641 taccttagcc agttcgtttt cgccagttcg atcacttcat cgccgcgtcc gctgatgatt
     8701 gcgcgcaaca tatacaagct aaatcctttg gcctgttcga gtttgatctg cggtggaatg
     8761 gctaactctt ctttggcgac caccacatcc accagcaccg gaccgtcgat ggagaaggcg
     8821 cgttgcaggg cttcatcaac ttcagacgct ttttctacac ggatacccgt aatgccgcac
     8881 gcttcggcaa tgcgggcaaa gtttgtgtcg tgtagttcgg tgccgtcagt caaatagcca
     8941 ccagctttca tctccatcgc cacaaagccc agcacgctgt tgttaaagac gacaattttc
     9001 actggcagtt tcatctgcac tactgagagg aaatcgccca tcaacatgct aaaaccgcca
     9061 tcgccgcaca tggcgaccac ctgacgttct ggctctgtcg cctgcgcacc cagcgcctgc
     9121 ggcatggcgt tagccatcga accgtggtta aacgaaccta acaggcgacg cttgccgttc
     9181 atttttagat aacgtgccgc ccacaccgtt ggcgtaccaa cgtcacaggt gaaaatagcg
     9241 tcatcggcgg caaaatgact aatttgctgc gccagatatt gcgggtgaat ggctttctcg
     9301 ctcggtttag ctaaatcgtc cagccctttg cgggcgtcgc ggtaatcttc cagcgcttta
     9361 tccagaaact tgcgatcggc tttttcttcc accaatggaa gcaatgcacg cagagtcgac
     9421 ttgatatcgc cgaccagtgc catatccacc ttgctgtgag cgccgatgct ggctgggttg
     9481 atatcaatct gaatgatttt ggcatcggtc gggtagaagg cgcggtaggg aaattgcgtg
     9541 ccgagtagca ctaacgtgtc ggcgttcatc atggtatgga aacctgacga gaagccgatt
     9601 aacccggtca ttccaacatc atacggatta tcgtattcga catgttcttt accgcgcagg
     9661 gcatgaacaa taggcgcttt aattttcccg gcaaactcaa ctaactcttt atgcgccccc
     9721 gcgcagccgc tgccacacat cagggcgata ttgctggaat aacgcagcag ttgcgccagt
     9781 ttgcgtaact cttcttcttc cggcgtcacg actggttgtg gcgcatgata ccagtgcgtg
     9841 gttgcccctt ctggcgcagg ttttaacgcc acgtcgcctg gtaacacgac aaccgaaacg
     9901 ccacggttaa gcaccgcttt gcgcatggca atcgccagta cttgtgggat ctgctccggg
     9961 ctggaaacca gctcgcaata gtgactacat tcgcggaata gctcttgtgg gtgggtttcc
    10021 tggaaatagc cgctgccaat ttcgctggag ggaatatgag cggcaatcgc cagtaccgga
    10081 acgtgattgc ggtggcaatc gaacaggccg ttgattaagt gcaggttgcc ggggccgcac
    10141 gatccggcgc agaccgccag ttctccgcta agttgtgctt cagcgccagc ggcaaaggcc
    10201 gccacttctt cgtggcgggt ggacatccac tcgatggtgc ccatgcgatt aagactgtca
    10261 ctaagaccgt tcagagagtc gcctgtgact ccccagatgc gtttcacccc tgccgattcg
    10321 agtgttttgg cgatataagc tgcaaccgtt tgtttcatgg ttctccatct cctgaatgtg
    10381 ataacggtaa caagtttagt tcatctgacg gagggggaag ggatgggaga gaaaggaggc
    10441 actaacggtt aaatagcccg atgaaaggaa tatcatcggg cataaggcga ttatgcgaga
    10501 accaaatccc cctgcggatg gcaggagcag gccagtacgt aaccttcagc gatttcggcg
    10561 tcggtcagcg tcattgtact gctcaccgta tattcaccgg aaaccacttt tgtcttacag
    10621 cagccgcaaa cacccgcacg gcaggcagcg acaaccggaa cgttattgct ttccagcgcc
    10681 tccagtagcg tggtgccaac cggggcgtaa aattctcgtg ccggttgcag tttggtgaat
    10741 ttcagaccgc tggtcgccgc ttctgctact ggggtgaaga atttctcttt aaagaaacgc
    10801 gtcacgccga gcgctttcac ttcctgctct acccaatcca tatacggagc cgggccgcag
    10861 gtcatcacgg tacgtgaagc taagtcaggt acacctgcca gcagttcgcg agtgagacga
    10921 ccagcgataa agccttcggt aacgttattt tctgccacca gcgttaccgg atagttacgc
    10981 cactcatcgg cgaaaataac atcctgcggc gtacgcacgt tgtagatcac ccgcacatcg
    11041 gcctgtggac ggttcttcgc aagccagcga cgcatcgaca taatcggcgt gacgccgcag
    11101 cctgccgcca gcaacaggaa tttatcttct gctttatcgt cgcaggtaaa ttcccccatc
    11161 gcgtccgaaa gccagagata atcaccgcgt tttacatcgc gcgtcagcca ctgggagccg
    11221 acaccgtcat caatccgccg cacggtcagg gtgatatatt cactcacgcc tggcgtggag
    11281 gaaatggtgt aagcacgcag cgtttccgct gagtttcgca cgctgaccag tgcatattgc
    11341 ccggcgcgat atgggtagta atcgtggcaa atcagggaaa tcgtccacac atccggcgtt
    11401 tcttgcgtaa tgtgatgaac ctgcatccgc cacgggcatt gattcgttgg catcgtcatc
    11461 gacaaactcc ttacgcgctc aacagttgct tcatgtcttc ttcaacagtg gtgatagaac
    11521 gcaggccgaa tttctcgttc agcaccgcca gcaggtctgg tgtcaggaaa ccaggtgcag
    11581 tcgggccggt gacgatattt ttcacgccca gagaaagcag cgtcagcaga atgacgatcg
    11641 ctttctgttc aaaccaggag agcaccagcg acagcggcag atcgttgaca ccgcagccca
    11701 gtttctctgc cagagtgaca gccagaataa tcgctgagta agcatcgtta cattgacctg
    11761 catctaccag acgcggcaga ccttcgatat cgccaaactc aagtttgtta aagcgatatt
    11821 taccacaggc gagggtcagg atcaggcagt catccggcac gctggtggcg aaatcggtga
    11881 agtagtggcg ctcgccgcgt gcgccgtcac agccaccaag caggaagata tgacgcagtt
    11941 tttcacggct caccagatca atcagcgtat cagcagcgcc aagcagcgtc tggcgaccaa
    12001 aacccacggt gataaggtgc ggaatttcgc tgtacgggaa gcctgccatc tgttgcgcct
    12061 gggtgataac cgcagagaaa tcatcaccgt ccagatgacg cacgccaggc cagccaacaa
    12121 tgctgcgggt ccagatacga tcgtcataag cgcctacggt tgggtcgatg atgcagttcg
    12181 aggtcatcac gatggggcca gggaaacgag cgaactccac ttgctgattc tgccagccgc
    12241 tgccgtagtt accgaccaga tgcttgaatt tacgcagctc cgggtagcca tgcgcaggca
    12301 gcatttcgcc gtgggtgtag acattaacgc ccgtgccttc ggtctgttcc agcaggttgt
    12361 agagatcttt gagatcgtga ccggaaatca gaatgcattt acccgccgtc gctttgacgt
    12421 tgacctgggt tggcgtcggg tgaccgtatt taccggtttc gcctgcatcc agaatgctca
    12481 tcactttgaa gttcatctgg ccgatttcca ttgaacactc aagaagcgcg ttcatatcgg
    12541 caggccaggt ccccagccac gccatgattt tatggtactg ggcataaata tcgttgtcgt
    12601 attgaccgag aacatgcgcg tgttccatat aggccgccgc acctttcagg ccatacaggc
    12661 acagcagacg caggccgaga atgttttcgc caatcgccgc tttatctttg ttaggggtaa
    12721 attctgctgc ctgacgttgc agctcgccga gatcgtcgct caccagttgc aggtcagcca
    12781 tcgggttatc gacgcgcgcg ttggcatcta cagccaggca ttgcgctttc agcgcctcgc
    12841 gcagggcaat cgcttcacga gcgtagccga caatacgcgg agaatcgaag ttaacgttgg
    12901 tcagggttga gaaaaaggca cgtggcgcga agctgtcaac atcgtggttg atgatgccgt
    12961 attcacgcgc ttttaccgcc caggcagaaa gcccttgcag cgccgcgatg agtaaatcct
    13021 gaaggtcaga agtttccgcc gttttaccac acatcccctg cgcgtatgag cagccgtttc
    13081 ctgccggagt acggatagtt tgttcacatt gcacacaaaa catgatcaca ccttttaaag
    13141 ttatatttaa tatacatgtt taaggttaag acgcttaacg cggggataaa agggattttt
    13201 catgcaactt taagggagat tgatttagcg caattttggc ggcagggatc taccgccaga
    13261 gaggtattac gcagagaaaa aggcgatgag gatcggcact aacaggctaa gaataaaacc
    13321 gtgaacaatt gccgccggga ccatatccag cccgccagta cgttgaagaa cgggcagggt
    13381 gaaatccatt gatgtggcac cgcataagcc cagtgcagta gagcggctgc ggcgaatcag
    13441 cccagggatc aacataatag caatcagttc acgggccaga tcattaaaaa acgccgcgct
    13501 cccgattacc gggccaaaag attcggtcaa taaaataccg gaaagagaat accagccgaa
    13561 accggaggcc attgccagcg cggtattgat ggggagatca agaataaagg cgttaattaa
    13621 accaccaatt aatgaactgg caaccaccac cacggcgaca atcattcccc ggcgattaag
    13681 gacaatctgc tttaaggtca tgccattatt gcgcaactga ataccgacga ggaaaagtag
    13741 cagaattaac gtgtattcgc tggcttcggt cgcgtgttgt aagaaagcca gtccactcag
    13801 accaatggca aaaccaatca ctactacgcc gcacagtttt agcgactcca gcgccatcgc
    13861 aatacgcgac gggagttttt cttgctgatg gtgattgcgc catggcaggc ctcgctccag
    13921 ccacatcagg gcggcaatat tacatagtaa aataacggta atactgacag cagaataatg
    13981 cagaatcgcc aacaggttac tggcgaggtt atcgagaaac gccagactga tacccataaa
    14041 aaagagaata aggtaaacca tccagcttaa tagctgatta ataactttta atgcagcttt
    14101 ttggcgaagc ggaatgaggt aacccacaat caggggaacc agaatgatta acagcccaga
    14161 aaacatgaaa acccagtcct tgcaaagatg aagtcgaaat gcgcgatgac acactactga
    14221 aagcggaagg acgagtaaag ttgcaattaa aaggaaatgt tatgcataag gagcagtaga
    14281 gtattcgttt tcatttaaag atattcttgc gctttaatta caaactgcac cgatgttggt
    14341 ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg at
//
LOCUS       DAZFZC010000115        14225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFZC010000115 DAZFZC010000000
VERSION     DAZFZC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.116"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..839)
                     /locus_tag="ACHKXP_004146"
     CDS             complement(<1..839)
                     /locus_tag="ACHKXP_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7649037.1"
                     /translation="MTGSEMAKVGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVVSEWATLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCV"
     gene            complement(836..1375)
                     /locus_tag="ACHKXP_004147"
     CDS             complement(836..1375)
                     /locus_tag="ACHKXP_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO7649038.1"
                     /translation="MQPLTYQQTSGFIPTAVINRSQIKQVPGHEKIRDAIRAWSAVDN
                     QDVVAALIVNEYRAQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLKYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(1406..1633)
                     /locus_tag="ACHKXP_004148"
     CDS             complement(1406..1633)
                     /locus_tag="ACHKXP_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7649039.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            1744..2436
                     /locus_tag="ACHKXP_004149"
     CDS             1744..2436
                     /locus_tag="ACHKXP_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7649040.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVSHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            2518..2991
                     /locus_tag="ACHKXP_004150"
     CDS             2518..2991
                     /locus_tag="ACHKXP_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649041.1"
                     /translation="MEKPSFPPLLTAGFHDMDSDGIKSLCVDTFPKSVRRSMLYCNFI
                     QLIEQFKLVNQQCLCFSEVWIDGSFTTEKPEPDDIDILVVVDYLALNSLPNTLMPLVS
                     SLLNRDFIKENYSIDVLLLPENHPEIDYSERRSYWRGWFGFDRKENPKGLVRVML"
     gene            2988..3920
                     /locus_tag="ACHKXP_004151"
     CDS             2988..3920
                     /locus_tag="ACHKXP_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649042.1"
                     /translation="MSEEKTFNSLKERIAFIQRDVDLLAQNKGRSFADDIIFRSLDSH
                     LSDLRERQRAIDNQHPLRDFMELRLKGVLVDFGSIPLDILSVISGNLASLVQKAVYRL
                     GSGKDSSRVPIDVKKSLDMRLADLRPGSTKLGVTFSTGSCELIETVSSHAVKEIFALL
                     SSIDEETFTAKIAEIGSQSTANLKNIINECEKYSLNFDLTWIGPLSDGTRQVSISSED
                     IKKLNARLALTKVIQLPDEHISGELAILSMYGKLEIASELGKIKASYPIDMLGEIQSK
                     YKVGERISLVVSVSEIHNERLGTSRRNYMVKKIE"
     gene            4405..4611
                     /locus_tag="ACHKXP_004152"
     CDS             4405..4611
                     /locus_tag="ACHKXP_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO7649043.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4687..4983
                     /locus_tag="ACHKXP_004153"
     CDS             4687..4983
                     /locus_tag="ACHKXP_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7649044.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIARHQVDIDSEV"
     gene            4989..5774
                     /gene="bet"
                     /locus_tag="ACHKXP_004154"
     CDS             4989..5774
                     /gene="bet"
                     /locus_tag="ACHKXP_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7649045.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAS"
     gene            5771..6451
                     /locus_tag="ACHKXP_004155"
     CDS             5771..6451
                     /locus_tag="ACHKXP_004155"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7649046.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            6448..6609
                     /locus_tag="ACHKXP_004156"
     CDS             6448..6609
                     /locus_tag="ACHKXP_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO7649047.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWVFPGLSVIRNPLKAQRLAEA
                     INNKRGLYD"
     gene            6602..7159
                     /locus_tag="ACHKXP_004157"
     CDS             6602..7159
                     /locus_tag="ACHKXP_004157"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7649048.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            7236..7451
                     /locus_tag="ACHKXP_004158"
     CDS             7236..7451
                     /locus_tag="ACHKXP_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7649049.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7550..7768
                     /locus_tag="ACHKXP_004159"
     CDS             7550..7768
                     /locus_tag="ACHKXP_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7649050.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            7816..8094
                     /locus_tag="ACHKXP_004160"
     CDS             7816..8094
                     /locus_tag="ACHKXP_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7649051.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            8066..8131
                     /locus_tag="ACHKXP_004161"
                     /pseudo
     CDS             8066..8131
                     /locus_tag="ACHKXP_004161"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8293..9456
                     /gene="intD"
                     /locus_tag="ACHKXP_004162"
     CDS             8293..9456
                     /gene="intD"
                     /locus_tag="ACHKXP_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO7649052.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKELPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9472..9548)
                     /locus_tag="ACHKXP_004163"
     tRNA            complement(9472..9548)
                     /locus_tag="ACHKXP_004163"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9512..9514),aa:Arg,seq:tct)
     gene            9791..10423
                     /gene="fimZ"
                     /locus_tag="ACHKXP_004164"
     CDS             9791..10423
                     /gene="fimZ"
                     /locus_tag="ACHKXP_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO7649053.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(10426..10941)
                     /gene="sfmF"
                     /locus_tag="ACHKXP_004165"
     CDS             complement(10426..10941)
                     /gene="sfmF"
                     /locus_tag="ACHKXP_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO7649054.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(10952..11929)
                     /gene="fimH"
                     /locus_tag="ACHKXP_004166"
     CDS             complement(10952..11929)
                     /gene="fimH"
                     /locus_tag="ACHKXP_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO7649055.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLNDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            complement(11971..>14225)
                     /gene="fimD"
                     /locus_tag="ACHKXP_004167"
     CDS             complement(11971..>14225)
                     /gene="fimD"
                     /locus_tag="ACHKXP_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
                     /protein_id="HFO7649056.1"
                     /translation="PELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIALLSS
                     AHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSWNYF
                     RGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDNMYP
                     DSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDVTID
                     ERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGLPQE
                     FTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFLYAK
                     SMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYHNLR
                     FSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSLSFS
                     WNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSWLAG
                     VGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNWQLS
                     GGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVYRYN
                     RIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPFGSL
                     VRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQAVTV
                     ISAVCTHPGS"
BASE COUNT         3973 a         3374 c         3285 g         3593 t
ORIGIN      
        1 acgcacatgt cgcggtggtt acgtccgtca cgttcacgca tcaggcggat atcgttagcc
       61 caccctgcaa aattcggttt tctggctgag ggcgcgatgg tcttcaccat gtcaaacatc
      121 cactctgcgg cggtcaggtc ttctgctgtc ccccacttgc tgccgctctg aattgcagca
      181 tctggtttca ccacaggaag atcgttttct ggttggtcag aggattcgcc agaattctcg
      241 gacgaaaaag gttttatatt gtcttttgtt agtttgtctt ttgtgtttac ctgattcggg
      301 taaacgcctt tacctgattt gggtaaactt ttcttacctg attcaggtaa atttacctct
      361 ttcaggtaaa ctttattttt cttacctgat tcgggtaatg ttgcccattc actgaccaca
      421 ttattaatac cgatattccg cccgctctga atcaaaatcc cacgctttac cagaacactt
      481 tttgcagcag aacacttgtg cggcaatatc ccggtcaatt cggacagttg ctcgttgctc
      541 acccaatcca gttttttatt aaagccatat gttttgcgca tgacagccag gaagaccaga
      601 agctggtgct gtgttaatcc ggccagcatc acagcttcca gcaactcatt tgcaatgcgc
      661 gtataaccat catcgagatc tgccacgcgc ggctcctttt gtgccgcatc cggcactgga
      721 aaattgaata tctcagcagt gtttgccata attcctcccg caatgagtgt gttacgattt
      781 gcacctgaaa gtcggttctg ttcccgcaga ccgactttcg ccatttctga acctgtcata
      841 ttgcccccag catggtggtc accatcgcca ttaatggacc agccaaatcc gggtccacac
      901 gaaacatcga cacaatgcct tcactcatct ccttcagttt ctggtggcgt ggtgcgttga
      961 gaatgacagc ctgttttgcc tcactgagtt ccttttccat ttcagccagc cgagccatga
     1021 agctatcctg ctcaaccagg tggccgcgat atttcagcgg tagtactgcc agaattgctg
     1081 gagtcagttc acgcacgtta tttcggtatt tttcagaatc gaatttgtta tcgagaaagc
     1141 ggaacagctt ctggcgtgca cgactgacat catcaggaaa atcgatagtg ccgccgccct
     1201 gtgctcgata ctcattcaca atgagtgcgg caacgacatc ctgattatct acagccgacc
     1261 aggcgcggat ggcatcacgg attttttcgt ggcctggcac ctgttttatt tgagaacgat
     1321 ttatcaccgc agtcgggata aatccgctag tctgttggta tgtaagtggt tgcatagtca
     1381 ttgccttatc agttaacgcc gcagtttagg cggcagaatt actcgcgtta aacaatggtg
     1441 cgaggtcggg acgaatatct gctggtttaa tctttccacc agtggctgag acaattttca
     1501 ttacatagcg ggcatcaatt ccgccaccgt gtagccaacg ccaaacagtg ggttgggcta
     1561 caccgcatag atctgccagt cgtttttgac tacctgtaat actgattgcg agttgaatgg
     1621 tttgatttgt cattatcaat tcctattggt attgcaatga atgaataata gcaatgcgta
     1681 ttaatccaag caatagcaaa acatgttttg accatcaata cgcaagcgta taaattaaaa
     1741 cttatgaaaa aagaaactct tgctgatcgc ttaaacctag cgatggaaca atctggaatg
     1801 tctcaaggcg ctcttgcaaa ggcgtctggc gtagctcaac ccacaatctg gagactgaca
     1861 agcggcaacg cgcgcggctc aacaaaaatt gttgaaatag ctaatgcatt gggtgttcga
     1921 acagaatggc tctcatcagg cataggcccg atgagaaatg acggtcaaca atcagggaag
     1981 cctgctgtca gccattccaa atacttcaaa attgacgttc ttgatataga agtcagtgcc
     2041 gggccgggtg taatcaaccg tgagtttgta gaagttctac gctcggttga gtactcgttt
     2101 gacgatgctc gtcacatgtt cgatggcagg aaggcggaaa atatccgcat cattaacgtg
     2161 cgtggtgaca gcatgtcagg aacgatcgaa ccaggtgatc tgctgttcgt tgatatcacg
     2221 gttaaatctt tcgacggtga tggtatctat gcgtttctgt acgacgacac agcccatgta
     2281 aagcgcctgc aaatgatgaa agataagcta ctggttatct ctgataacaa gagctactca
     2341 ccgtgggacc cgatcgagaa agacgagatg aaccgggtgt tcatcttcgg taaggttatt
     2401 gggagcatgc cgcagacgta caggaagcat ggttaataat tagcctgtag ctttttaagg
     2461 cacactcttt tcaagaatat taaatagaac gctatatggc ataaacaagg acttaggatg
     2521 gaaaagccta gcttcccacc tcttcttaca gcaggatttc atgatatgga tagtgatggc
     2581 atcaagtctc tttgtgtcga caccttccca aaatccgtta gaagaagcat gctatactgt
     2641 aattttatac agcttattga gcaatttaaa cttgttaatc agcagtgtct atgtttctct
     2701 gaagtgtgga tcgatggttc attcactaca gagaaacctg aacctgatga catagatata
     2761 ctcgtggtcg tcgattattt ggccctaaac tctttgccaa acacgctgat gccattggtt
     2821 agctcattgt taaatagaga ttttattaaa gaaaactaca gcattgacgt tcttttgctg
     2881 cctgaaaatc atccagaaat cgattatagt gaaaggcgaa gttactggag agggtggttt
     2941 ggttttgatc ggaaggagaa tcctaaaggc ctcgtgaggg taatgttatg agcgaagaaa
     3001 aaacattcaa ctccctcaag gagcgaattg cgttcattca acgcgatgtg gatctgcttg
     3061 ctcaaaataa ggggagatca tttgctgatg acattatatt tagatcactt gactcccatc
     3121 tgagcgatct ccgagagaga caacgtgcaa tcgataacca acatccttta cgtgatttca
     3181 tggagttgag attaaaaggt gttttggtcg atttcgggtc tattccgtta gatattctat
     3241 ctgtaatttc aggtaatctc gcatcccttg tacagaaagc agtttaccga cttggatctg
     3301 ggaaagattc tagtcgtgtg ccgattgatg ttaaaaaatc attagacatg agattggcag
     3361 acttgcgtcc aggttcgacg aagttaggtg ttactttttc tactggttct tgcgagctca
     3421 tcgaaacagt atccagtcat gctgttaagg agatttttgc actactctca tccattgatg
     3481 aagaaacatt cacggctaaa attgccgaaa ttggttcgca atcaacagct aacctaaaaa
     3541 acatcataaa tgagtgcgaa aaatatagtc ttaactttga tcttacttgg attggacctt
     3601 taagtgatgg taccagacaa gtgtcaattt cttcagaaga tataaaaaaa ctgaatgctc
     3661 gcttagcttt aacgaaggtc attcagctac cggatgagca tatatcaggc gaacttgcta
     3721 ttttatccat gtacgggaaa ttggagattg caagcgagct tggaaaaata aaggcttctt
     3781 atccgattga tatgttagga gaaattcaaa gtaaatataa agttggtgaa cgaatatcgc
     3841 tcgtagtaag cgtttctgag attcacaatg aaagattagg aacatcacga cgtaactaca
     3901 tggttaaaaa aatcgaatag tgatgctacc cggccaccgt gccgggtttt cttttgcctc
     3961 cccctcatca cacaaaccgt tcgaaaaacc accataaccc ccgcttcagt tatcgctatg
     4021 cgatgcaagt cacaaaatta attctttttg ctatcaaaca tttaatatca aaacacatca
     4081 gccaatagcg ataagtattg atatcaccaa tagcaatagc tattatcacc atatcgcaac
     4141 aacacaacga tacggcaacc acctgattca ccgttgcgat gaccgcttag atccgcagct
     4201 tgaatttcag caggctccgg ggagtgcgag gggtgaagcg gacgcgtgaa cgtcggtgtg
     4261 accagctgaa atcaactcaa cacttcatac ctcagtcgct tcaacgaggc gacttagtta
     4321 tgacaaccgg cggccatcca ccgcctgaat acgcgcagaa gtctcaatat gttcagcagc
     4381 ccagcttacg ggcaggagtt ttttatggtt catcaacatt acggaacgca gaccgttaat
     4441 cgaggtgcgg tcatgccagg aatgctggtc aaacacaaag atggtacctg gactgcatca
     4501 gctaatttac gcggacggct ttatctgcat cgcggcatcg agcgcactta tacccgtgat
     4561 ttgctcgtag aagtttttct cgacggacgc ggtaacggcc tgaatcacta atcccctttc
     4621 ctgttttcct aatcagcctg gcatttcgcg ggcgatattt tcacagccat tttcaggagt
     4681 tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg ggcgcgtcac
     4741 taccagcaga tcgcccgtga agagaaagag gcagaactgg cagacgacat ggaaaaaggc
     4801 ctgccccagc acctgtttga atcgctatgc atcgatcatt tgcaacgcca cggggccagc
     4861 aaaaaagcca ttacccgtgc gtttgatgac gatgttgagt ttcaggaacg catggcagaa
     4921 cacatccggt acatggttga aactattgcc cgccaccagg ttgatattga ttcagaggta
     4981 taaaacggat gagtacagca ctcgcaacgc tggcagggaa gctggctgaa cgtgtcggca
     5041 tggattctgt cgacccacag gaactgatca ccactcttcg ccagacagca tttaaaggcg
     5101 atgccagcga tgcgcagttc atcgcattgc tgatcgtcgc caaccaatac ggccttaatc
     5161 cgtggacgaa agaaatttac gccttccctg ataagcagaa cggcatcgtt ccggtggtgg
     5221 gcgttgatgg ctggtcccgc atcatcaacg aaaaccagca gtttgacggc atggactttg
     5281 aacaggacaa cgaatcctgc acatgccgga tttaccgcaa agaccgcaat catccgatct
     5341 gcgttaccga gtggatggat gaatgccgcc gcgaaccatt caaaacccgc gaaggcagag
     5401 aaatcaccgg accgtggcag tcgcatccca aacggatgtt acggcataaa gccatgattc
     5461 agtgtgcccg tctggccttc ggatttgcgg gtatctatga caaggatgaa gccgagcgca
     5521 ttgtcgaaaa taccgcatac actgcagaac gtcagccgga acgcgacatc actccggtta
     5581 acgatgaaac catgcaggag attaacactc tgctgatcgc cctggataaa acatgggatg
     5641 acgacttatt gccgctctgt tcccagatat ttcgccgcga cattcgcgca tcgtcagaac
     5701 tgacacaggc cgaagcagtg aaagctcttg gattcctgaa acagaaagcc actgagcaga
     5761 aggtggcatc atgacaccgg acattatcct gcagcgtacc gggatcgacg tgagagctgt
     5821 cgaacagggg gatgatgcat ggcacaaatt acggctcggc gtcatcaccg cttcagaagt
     5881 tcacaacgtg atagcaaagc cccgctcagg aaagaagtgg cctgacatga aaatgtccta
     5941 cttccacacc ctgctggctg aggtttgcac cggtgtggct ccggaagtta atgctaaggc
     6001 gctggcctgg ggaaaacagt acgagaacga cgccagaacc ctgtttgaat tcacttccgg
     6061 cgtgaatgtt actgaatccc cgatcatcta tcgcgacgaa agtatgcgca ccgcctgctc
     6121 tcccgatggt ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg
     6181 ggatttcatg aagttccggc tcggtggttt cgaggccata aaatcggctt acatggccca
     6241 ggtgcagtac agcatgtggg tgacgcgaaa agatgcctgg tactttgcca actatgaccc
     6301 gcgtatgaag cgtgaaggcc tgcattatgt cgtgattgag cggaatgaaa agtacatggc
     6361 gaattttgac gagatggtgc cggagttcat cgaaaaaatg gacgtggcac tggctgaaat
     6421 tggttttgta tttggggagc aatggcgatg aagcatcctc acgataatat ccgggtaggc
     6481 gcgatcactt tcatctactc cgttacaaag cgaggctggg tatttcccgg cctttctgtt
     6541 atcagaaatc cactgaaagc acagcggctg gctgaggcga taaataataa acgggggctg
     6601 tatgactgat ttcaccggaa gcaatactcc tgccgaacat cgcgacagct ggcgcacacc
     6661 accagagatt tttgctgcgc ttaatgcaga gttcgttttt caacttgatg ctgccgccag
     6721 cgaaaaaaac cgactatgtc ggctttttat ctcacaggag cagaacacat taaccacttc
     6781 atggcctgaa gcaatgggat atgcctctgg ttatgtctgg ttgaatccac catacagcaa
     6841 tatttcccct tttgtgaaaa aggcagccac tgaaaacaaa ttcagtagtg tgggatgtgt
     6901 aatgttattg cctgctgaca catctgtcgg atggtttcat gaagcgatac aaaccgccag
     6961 tgaggtcaga ttcatcacgg caggacgact ggcatttatt aacccactca ctgagaaacc
     7021 cgtcagtgga aataataaag gctcgatgct cattatctgg cacccatacc cccgtacaca
     7081 ctgccacttt acgaccgttg atcgtggaga gttgatggcg ttcggctcaa ggattcttgc
     7141 ccgtcgggag gctgcatgac aaccacggaa tgcatttttc tggcagcggg cttcatattc
     7201 tgtgtgctta tgcttgccga catgggactt gttcaatgac acctcagcag gaaaacgccc
     7261 ttcgcagcat tgcccgtcag gctaattctg aaatcaaaaa agccagacag cagtttccgg
     7321 ataaaaacgt cgatgacatt tgccgtagcg tactaaagaa gcaccgcgaa acggtaacgc
     7381 tgatgggatt cacaccgact catttaagcc tggcgatcgg catgttaaac ggcgtcttta
     7441 aggaacggtg aacatgaaaa gcaaaatcat cagggagcta caggctcctt ttttattgtt
     7501 cgcattcatc ctcaagcgta ttaaccaaca attcagggat taatggaaga tggcagacat
     7561 cattgattca gcatcagaaa tcgaagaatt acagcgcaat acagcaataa aaatgcgtcg
     7621 tctgaactac cagactgtat ccgcaactca ttgttgtgag tgtggcgatc cgatagatga
     7681 gcgaagacgc ctggctgttc agggttgtcg gacttgtgca agttgccagg aggagatcga
     7741 acttaagaac aaacaatggg gactgtgatg gcctcaaagc agcaaatttc aacatcgtcc
     7801 aactgaggtg taaaaatgtt cagaatcatt tttcctaaca cctggtacgt cgaccaccac
     7861 ggcactccct gcaaaatcct gcgttctacc cacaacaaag ttcactacat ccgaaaaggc
     7921 agaacatgta tcgccagcat gttccgcttt aatcatgact ttgaacctgt gaataaagct
     7981 gatgcagatc ggatagcaga agagatcgaa acggcagaac acattaagaa gttacgtgac
     8041 atgcgttcaa aaagcagagg taaccatgga atcatacagc ctcacactcg atgaggcctg
     8101 tcagtttctt aagatatcca gaccaaccgc caccaactgg atacgaacag gccgcctaca
     8161 ggcaacacgt aaagatccaa ccaagccaaa atctccttac ctcacaacac ggcaagcctg
     8221 cattgcggcg cttcagtctc cgctgcatac tgtccaggtg agcgcgggtg atggcataac
     8281 agaggaaaga aaatgtcact cttccgcaga aatgaaatat ggtatgcctc gtattcgctc
     8341 ccgggcggga aacgaattaa ggaatctctt ggcacaaagg acaagcggca agctcaggag
     8401 ttgcacgaca agcgaaaagc agaactctgg cgagtagaaa agctagggga tttacctgat
     8461 gtcacttttg aagaggcctg cctaagatgg cttgaggaaa aagctgataa aaaatctctc
     8521 gattcagata aaagccggat tgagttctgg cttgaacatt ttgagggtat aaggcttaaa
     8581 gatatctcgg aggcaaagat ttactctgct gtaagcagaa tgcataacag aaagacgaaa
     8641 gaaatatgga aacagaaagt tcaggccgcc atcaggaaag gtaaagaact gcctgtttat
     8701 gaaccaaagc cagtatcaac tcagacaaag gcaaagcatc ttgccatgat aaaggccatt
     8761 ctccgtgctg cagaacgcga ctggaagtgg ctggaaaaag cgcctgtcat caagatacca
     8821 gcggtcagaa acaagcgagt cagatggctg gaaaaggagg aagcaaaacg ccttattgat
     8881 gagtgccccg aaccactgaa atctgtcgtc aagtttgcgc tggcaactgg tctgagaaag
     8941 tcgaacatca taaatctgga atggcaacaa atcgacatgc agcgacgagt tgcctgggtg
     9001 aatccagaag agagcaaatc aaaccgcgcc attggtgtgg cgctaaacga taccgcctgt
     9061 aaagtgttgc gtgatcaaat aggcaagcat cacaaatggg tgtttgtaca taccaaggcg
     9121 gctaagcgag cagatggaac atcaacgcct gcggtcagga agatgcgcat cgacagcaag
     9181 acatcatggc tatcagcttg tcgtcgtgca ggaattgaag atttccgttt ccatgacctc
     9241 agacacacct gggcaagctg gctgattcag tcaggcgtcc cattatcagt gcttcaggaa
     9301 atgggcggat gggagtccat agaaatggtt cgtaggtatg ctcaccttgc gcctaatcat
     9361 ttgacagagc atgcgaggaa aatagacgac atttttggtg ataatgtccc aaatatgtcc
     9421 cactctggaa ttatggagga tataaagaag gcgtaactga ttgaattgta atggcgcgcc
     9481 ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg ttgctctatc caactgagct
     9541 aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa ttatacggtc aacccttgct
     9601 gagtcaatgg cttttgatct ggttgctgaa caagtgaacg accgcgtctg attttctgat
     9661 ttatttcgct atagtggcaa acaaacgcac accgctgcgc gtctgaatca agaaaacccg
     9721 tattttcatg tatcaaagta caatttaccg ccctaacgga aaattgtccg ctcctatgag
     9781 actggtaact atgaaaccaa cgtcggtgat cattatggat actcatccta tcatcagaat
     9841 gtctattgaa gttctgttgc aaaaaaacag tgaattgcag attgtcctga aaacggatga
     9901 ttaccgcata accatcgatt atctccgaac ccgtcctgtt gatttaatca ttatggatat
     9961 agacttgccc ggaacagacg gttttacctt cctgaaaagg atcaaacaaa tccagagcac
    10021 agtgaaagtg ttatttttat catcgaaatc agaatgcttt tatgctggca gagcgataca
    10081 agctggtgct aacggttttg tcagtaaatg caatgatcag aatgatattt ttcatgccgt
    10141 tcagatgatc ctctccggat acacgttttt tcccagcgaa acgcttaact atataaaaag
    10201 caataaatgt agtacgaata gttcaacgat cactgtgcta tctaatcgtg aagtgaccat
    10261 attacgttat ctggttagcg gattatctaa taaagaaatt gccgataagt tattacttag
    10321 caataaaaca gttagtgcgc ataaatctaa tatttatggc aagctaggtt tgcattcaat
    10381 tgtagagctt atcgactacg ccaaattata cgaattaata taatattaat tataattgat
    10441 cataaatatc gcatccgctt tcgccacacc tggccgaaca ccgctggcta acgctcgata
    10501 attggcaaaa aagtttagtg tgacattgcc atttgcatct acttcctcag tcgggctcgc
    10561 ctcccccagt gcgagccggg agcgatcgct attacgtaat tcgatggcga cggtttgtgc
    10621 cattgcggga tcatccagag ccagcaggtt ggtatcggat gccggcgttc ccgtaaataa
    10681 aatcgcaact gaacccggag gacatccctc cagccgcagg ctaaaaggga cgagtgccgt
    10741 ggtatcgcca gcgttcagta attgtgtcgt aggccatctg cctaaatcta ccgtcttatc
    10801 aatatccgct gtgtttacgg tacaggagaa atcaacaacg ttaccgtgca aattgatatt
    10861 aatcgttcct aaagggtcag ctgcccatcc aatggaactc cacagtaacc cgcagaaaca
    10921 gctaaagagt actcttctca ttctccgtac ctcattgata atcgacgcgt aaatacccca
    10981 gcgcgctaaa cggcccctcg gtcggttttt gaccggtaat actgataggc caggcgcgaa
    11041 gtgtgacatt ggctgccgca gctgcatcca gacggaaagg aataacgcta ttgagatcgt
    11101 taggcgtaat cggcgtatcg ttctgatcgg cgacaataaa acctaaatcc tgattgtccg
    11161 acaccatcgc ctgaccagaa acggcactgg cttccagacg cattgttaaa taagcctgcg
    11221 cagcaacatt cgtacatttg accgcaatgc tcttggtttg cggcatgacg ccagctggtc
    11281 gattccccgg ccctgccgca ctaaataacg atgcgccgat atcaccaaaa tcaaattcaa
    11341 caatctgccc ggcatttaat tcgcagtttt gcggcacttc aacccggcca ccaaaactaa
    11401 tggtataaac aggtgtaacc aacggatcgt aaggcgttga tgtcacatac acggtaaaca
    11461 tcacctgtct ggggatctcc accatgttaa taaagggacg aataaccttt aaacgaaata
    11521 ttaaacgaga atccatcacc ccgaatggaa ggttttgcga aacgttagga tctgttccca
    11581 tccgaatata attttgcggg gggaactgaa tatccataac actatcaacc agactcatcg
    11641 cacctaatag ataatcattt aattgcatat atttataatt atcgatagtt tcctgaacaa
    11701 taaagttagt gacataacta cggtatgtat aattcaccag cgtgccgggt ggacaaattg
    11761 ctgagacacc tacccagccg gatttttccg gtaaaacaac aatctgccct ggctggttat
    11821 tggtgctatt aaagatattt gagagatcat agtgttcatc ttctgctacg ccagtactat
    11881 ttgcacaaac agtagcccag gatatgtttg ccagacacaa acatgccatc gccaataata
    11941 atctgcagat tatcttattg ataatttcct ttatgagcca ggatgtgtgc aaactgccga
    12001 aataacagtg acggcttgct gtaagctttg cttcggcaat acatagtgcg caatgcagcg
    12061 tgagttcgcg ccgtctcccc actgaaccag taattcacca gacaatggcg caccacttaa
    12121 ataaacttgc ccgtcatcac ccaccatact ggttattccg gtactgtttt cccgtaccag
    12181 tgatccaaac ggcaccggtt ttccgccctg ggtaacggta atgagcgccc gcacgcctat
    12241 gcgggtatca aaattggcac gaaccaacgc gccttgcgtc ggcacaacgc tgctaatatt
    12301 tttttcaaca tcgatggaat tccccatcgt attggtatca agcgcgatac ggttataccg
    12361 ataaaccgtg gcatacggca tcaccgcata gccgcgccag tcggttaaaa tgccagtttg
    12421 attttcaatg cgtacaccgc ctgcgccagg tgctttaatc aaaacattgg tatcccctaa
    12481 aggctggctc agcgttatgc cattttcatg cccgaccaca ccgccagaca gctgccagtt
    12541 aacgtcatgt tgatcgcggt cgtagttata cccgaccccc agcgtaccgt aagcggcctg
    12601 ccagtttgcc gtggcgctgc ccgtgtaccc attattcgag gtatcaccct ggcttacgtg
    12661 ataactcagg ttgtggcctt ccagtaaggt cccacctaca cctgccagcc agctattttg
    12721 cccgttgctg ttacggttgg cgttaaagga ggcataagcg cggtcgagcg cattttcccg
    12781 ggtgtagcga cgtttggtca aaacattgaa aggcactgaa acattaagtc cgacaatacg
    12841 ttcgttatcg gggatcccta cagattcatt ccacgaaaat gagagcgaat aactgatgcc
    12901 aacccagctg ctggtatacc ccacctgata ccacgtatct gaatccgaag tattccagta
    12961 tttttgatgg gtaccagaga tataaagcga gccaaagtca ttaagtgatt gtgaaatatt
    13021 taactgcaaa cggtctttac ggctaaagcg taaattgtgg taattcacga ttatcggttc
    13081 atcgcgatgc tcaccgtcgt aatcgtaatc atattcgtac ccctccattc gacgatacgc
    13141 aacatcatca agggtataaa aaccttgtgt cgaatagcgg taacccatta actgaaaatt
    13201 ggtgccgaag gtgttcattg atttcgcata gaggaagcga atggaatccc cctcatgacg
    13261 actgtcgtcg gctaactgac tgcgcgcatg cgttacatcc agcgacactg cgccccagtt
    13321 cccgaggttg cgccccagcc ctaacaaaaa ggcggtgtaa ttggcagata attgcgtccc
    13381 gccgtaggca gtaaattcct gtggtaaacc accgagtgcc gtgccctgaa aaaagaaagg
    13441 tgatgattgc tgactattac cgctacgaaa atcgcccgcc gtcagatcaa atttgaaacg
    13501 cccttcgcgt tgtaaaattg gcactgttga atacggaatt gtgtaattct gctgattgcc
    13561 atcgcgctcg tcgatggtga cgtccagatc gccatttgaa gatgtcgggt gcaaatctgt
    13621 aatttcaaaa gcgccggggg aaacatagct ttgatagata ataaaaccat tttgccgaat
    13681 cgttagctgg gccgccgtac gggcaatccc acgtaccgtt ggggcaaacc cttgctggct
    13741 atcaggatac atattatcag aagaataaag ccgtacacca cgaaatccaa cgccatcgaa
    13801 aatatcactt cctgtattgc cgtctcccat taccagttca cttttcagcg gaataatggc
    13861 gcgctgtacc caggtgccaa tattattcca ctgttctgaa tgatatccat ttccgcgaaa
    13921 atagttccag gaaccattgt tgcgtaaacg ccacgggcca atattaatcc cgctgagctc
    13981 actaaaaaaa tagctatcgt taccattacc tctgttaccg gtgaaattat aattcaggag
    14041 taaagcagga ataccttcat cccactcttc aggcggaatg taaccgtgag cgctactcaa
    14101 caacgctatc tgaggaatag tgatgttcag gcgcatcgct gcaaaatcaa agttaattgt
    14161 ggcatcaggt acagctttca gtaaattgat gcatttattg ttttgctgct gggctaactc
    14221 gggaa
//
LOCUS       DAZFZC010000116        13773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFZC010000116 DAZFZC010000000
VERSION     DAZFZC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.117"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..256)
                     /locus_tag="ACHKXP_004168"
     CDS             complement(<1..256)
                     /locus_tag="ACHKXP_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649057.1"
                     /translation="MLVNSWCNALNEAISTSATICAEDLYKGGHWATAKAILDDYPID
                     LWILSAGLGLLHHKDNVVPYKATFAVGYDESIPLYSQEYVG"
     gene            complement(461..694)
                     /locus_tag="ACHKXP_004169"
     CDS             complement(461..694)
                     /locus_tag="ACHKXP_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013367840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649058.1"
                     /translation="MKTFDTQLAMSIVSHSTGGFSYDTLARALISKDKNENPDDLLES
                     CRNFCLSLEEKGILRRCQRCSHSQDEYFEYIPH"
     gene            complement(797..2041)
                     /locus_tag="ACHKXP_004170"
     CDS             complement(797..2041)
                     /locus_tag="ACHKXP_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7649059.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGSGMHLLVHPNGSKYWRLQYRF
                     DGKQKMLALGVYPDVSLADARTRRDDARNLLANNIDPGDKKKSDKIEQEEARTFEQLA
                     VEWHATNKKWSEEHSRRVLKSLEDNLFPAIGKRNIADLKTRDLLAPIKAVELSGRLEV
                     ASRLQQRTTAIMRYAVQSGLLDYNPAQEMVGAVASSNRQHRPALQLKRIPELLRRIDS
                     YTGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETSMWTIPAEREAIDGVKHSQRGS
                     KMRTPHLVPLSHQALAILKDVYKLSGDRDFVFIGDHDHRKPMSENTVNKALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLDERRLML
                     QWWADFLDANRESEVSPYDFGK"
     gene            complement(2228..2312)
                     /locus_tag="ACHKXP_004171"
     tRNA            complement(2228..2312)
                     /locus_tag="ACHKXP_004171"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2276..2278),aa:Leu,seq:caa)
     gene            2508..3527
                     /gene="ahr"
                     /locus_tag="ACHKXP_004172"
     CDS             2508..3527
                     /gene="ahr"
                     /locus_tag="ACHKXP_004172"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO7649060.1"
                     /translation="MSMIRSYAAKEAGGELEVYEYDPGELRPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLIAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3657..5159
                     /gene="yjgR"
                     /locus_tag="ACHKXP_004173"
     CDS             3657..5159
                     /gene="yjgR"
                     /locus_tag="ACHKXP_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO7649061.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5278..6360)
                     /gene="lptG"
                     /locus_tag="ACHKXP_004174"
     CDS             complement(5278..6360)
                     /gene="lptG"
                     /locus_tag="ACHKXP_004174"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7649062.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6360..7460)
                     /gene="lptF"
                     /locus_tag="ACHKXP_004175"
     CDS             complement(6360..7460)
                     /gene="lptF"
                     /locus_tag="ACHKXP_004175"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7649063.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7523..7573)
                     /gene="ytgA"
                     /locus_tag="ACHKXP_004176"
     CDS             complement(7523..7573)
                     /gene="ytgA"
                     /locus_tag="ACHKXP_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO7649064.1"
                     /translation="MSIIILATISTTQENK"
     gene            7727..9238
                     /gene="pepA"
                     /locus_tag="ACHKXP_004177"
     CDS             7727..9238
                     /gene="pepA"
                     /locus_tag="ACHKXP_004177"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO7649065.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9496..9939
                     /gene="holC"
                     /locus_tag="ACHKXP_004178"
     CDS             9496..9939
                     /gene="holC"
                     /locus_tag="ACHKXP_004178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO7649066.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9939..12794
                     /gene="valS"
                     /locus_tag="ACHKXP_004179"
     CDS             9939..12794
                     /gene="valS"
                     /locus_tag="ACHKXP_004179"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO7649067.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12848..>13773)
                     /locus_tag="ACHKXP_004180"
     CDS             complement(12848..>13773)
                     /locus_tag="ACHKXP_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFO7649068.1"
                     /translation="VSADMPLVGCVLTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSF
                     NCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVVLMAIVGIVSIGI
                     FNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQ
                     ILNAYDSFVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNL
                     SLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTYLLGDL
                     DDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3516 a         3510 c         3595 g         3152 t
ORIGIN      
        1 tccctacata ttcttgagaa tataatggta ttgattcgtc atatccaaca gcaaaggtcg
       61 ctttataagg tacaacattg tctttgtggt gaaggagtcc taaaccagca gaaagaatcc
      121 ataaatcaat aggataatca tctaagatgg ctttagccgt cgcccagtgc ccacctttat
      181 aaagatcctc tgcacatata gttgcagatg tagaaatagc ttcattcaga gcattgcacc
      241 aagaattcac gagcacattt gaaggggttc ggcctgatga aaattgactc agactcaata
      301 tatccctact accgtagtgt ttgccgtttg tacatgtggt tattaagttg atgttgctca
      361 caatcgcccc cccagaatcg tgttacacaa gctacaggag aaaaaagggg atgacaagag
      421 cggagtaatc agtccctgaa aattttttca gggactgatg ttagtgagga atatactcga
      481 aatactcatc ttgagaatga gaacatcttt ggcatcgcct cagaatacct ttttcctcaa
      541 gagagagaca aaaatttcga cacgactcaa gaaggtcgtc aggattttca tttttatctt
      601 tagaaatcaa cgctctagct agggtgtcat aactaaaacc tcccgttgag tgcgatacaa
      661 tgctcatcgc aagttgagta tcgaaagttt tcatagtgca cctctgagtc gccagaataa
      721 tacagttata aaaaagtata ggttatagtt caagccggct tttcaaaatc gaacatagca
      781 agcatcagga attagactat ttgccaaagt cataagggct aacttcgctt tccctattcg
      841 catccaaaaa atcagcccac cactgcagca tcagtcgccg ttcatcaaga tgttcggcct
      901 tatgaatgta agctgcgcgt actgagttac gttccatatg gctcatctgc ctttccactg
      961 catccctcga ccacaatcca gactcgatca atgaactaca ggccattgtc ctgaaaccgt
     1021 gcccacaaac ctctaccttc gtgtcataac ccataacgcg cagcgccttg ttcaccgtgt
     1081 tttcactcat cggtttgcga tggtcatgat cgccgatgaa aacaaaatcg cgatcaccac
     1141 taagtttgta aacatcttta agaattgcta aagcttgatg agaaagcggc accagatgag
     1201 gcgtacgcat ttttgaacca cgctgtgaat gctttactcc gtcaatagct tcgcgctctg
     1261 caggtattgt ccacattgat gtttcgaaat cgatctcaga ccaacgagca aagcgtagtt
     1321 cacttgaacg aatgaatacc aggagcgtga gttcgacggc aagtctagta agaggtctac
     1381 cggtataact atctatgcgc cgtaatagtt ctgggattcg cttcaactga agagcaggtc
     1441 gatgctgtct gttacttgaa gcaacagcgc caaccatctc ctgcgcgggg ttatagtcca
     1501 gtaaaccact ctgcacggca tagcgcataa ttgccgtggt tcgctgttgt aatcgtgaag
     1561 caacctcaag ccgtcctgac agttcaacgg ctttgattgg ggctagcagg tctcgggttt
     1621 tgagatccgc aatattccgc ttaccaatag ctggaaacag attatcctcc aggcttttca
     1681 gtactcgcct gctatgttct tccgaccact ttttatttgt tgcatgccac tcaactgcga
     1741 gttgttcaaa agtgcgggct tcttcttgct caattttatc acttttcttt ttgtctcccg
     1801 ggtcgatgtt gtttgccaac aaattacgag cgtcgtcacg gcgagtccgt gcatcggcaa
     1861 gcgacacgtc aggataaaca cctaaagcca gcatcttttg cttgccgtca aaacggtact
     1921 gcaaacgcca gtacttagaa ccattagggt ggacaagaag atgcatacca ctgccatcag
     1981 ttaacttgta ttgcttatct gttggcttag ctgttctgac tttgatatct gttagagcca
     2041 tgcttatccc ctccctgttg gtacaagcat tatcgaaccg gaaataccat catctgtacc
     2101 aacatctgtt ggtagatgta cgttgaagtc ggttgacctt gagagagtta atatagcggg
     2161 aatgatgggt aaatactgga tttcaggcac aaaaaaagac gtccgttgac gtctattgat
     2221 gctccgatgg tgccgaaggc cggactcgaa ccggcacgta tttctacggt tgattttgaa
     2281 tcaactgcgt ctaccgattt cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg
     2341 gattatacct gtcgcacgcc accatgcaag cgccagcttg ctcgcacctc gctaagtgct
     2401 gaaattttca gcattgcata cagcgatgtg taacctttgt cacactccag gcaccccgcc
     2461 ctgccatgct ctacacttcc cagacaacac cagagaagga ccaaaaaatg tcgatgataa
     2521 gaagctatgc cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac gatcccggtg
     2581 agctgaggcc acaagatatt gaagtgcagg tggattattg cgggatctgc cattccgatc
     2641 tatcgatgat cgacaatgaa tggggatttt cacaatatcc gctgattgcc gggcatgagg
     2701 tgattggtcg cgtagtggcg ctcgggagcg ccgcacagga taaaggtttg caggtcggtc
     2761 agcgtgtcgg gattggctgg acggcgcgta gctgtggtca ctgcgacgcc tgtattagtg
     2821 gtaatcagat caactgtgag caaggtgcgg taccaacaat tatgaatcgc ggtggctttg
     2881 ccgagaagtt gcgtgcggac tggcaatggg tgattccact gccagaaaat attgatatcg
     2941 agtccgccgg gccgctgttg tgcggcggta tcacggtctt taaaccactg ttgatgcacc
     3001 atatcactgc taccagccgc gttggggtaa ttggtattgg cgggctgggg catatcgcta
     3061 taaaacttct gcacgcaatg ggatgcgagg tgacggcctt tagttctaat ccggcgaaag
     3121 agcaggaagt actggcgatg ggtgccgata aagtggtgaa tagccgcgat ccgcaggcac
     3181 tgaaaacact tgcggggcag ttcgatctca ttatcaacac cgtcaacgtc agcctcgact
     3241 ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt ccatacggtc ggtgcggttc
     3301 tcacgccgct gtctgttccg gcctttacgt taattgcggg cgaccgcagt gtctctggct
     3361 ctgctaccgg cacgccttat gagttgcgta agctgatgcg ctttgccgcc cgcagcaagg
     3421 ttgcgccgac caccgaactg ttcccgatgt cgaaaattaa cgatgccatc aagcatgtgc
     3481 gcgacggtaa ggcgcgttac cgcgtggttc tgaaagccga cttctgatcc ctttgccggg
     3541 tgcctgtcgc ccggcactta aatctctttg gaaggcatct cgcaatctgc tgaatttagc
     3601 ggtgaagagt gcgcagccgt tgcctatact ccaggcagga aactggagga tatctcatga
     3661 gcgaacccct gttaattgcc cgcacgccgg acacagaact gtttttattg ccgggaatgg
     3721 ctaaccgtca cgggctgatt actggcgcaa cggggacggg taaaactgtc acgttgcaaa
     3781 aactggcaga gtcattgtcg gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg
     3841 atctgaccgg catcgcgcag gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa
     3901 atatcggcgt caacgactgg caaccgcatg ccaatccggt ggtggtgtgg gatatctttg
     3961 gcgagaaagg ccatccggtg cgggcgacgg tttcggatct ggggccgctg ttgctggcac
     4021 gactgttgaa tctcaacgat gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg
     4081 acgatcaggg attgttgctg ctcgacttta aagatctgcg ggcgattacc cagtacatcg
     4141 gcgataacgc caaatctttc cagaatcagt acggtaatat cagtagcgca tcggttggtg
     4201 ccatccagcg cggactgttg tcgctggaac agcaaggcgc agcacacttc tttggcgagc
     4261 cgatgctgga tatcaaagac tggatgcgca ccgataccaa cggtaaaggc gttatcaata
     4321 tcctcagcgc cgagaagctt tatcagatgc cgaaactgta cgccgccagc ctgctgtgga
     4381 tgctttcaga gttgtatgaa caattgccgg aagcgggcga tctggagaaa ccaaaactgg
     4441 tgtttttctt cgacgaggca catctgctgt ttaacgatgc accgcaggta ctgctggata
     4501 agattgagca ggtgatacgg cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc
     4561 aaaacccgtc tgatattccg gataatgtgc tcgggcagct cggtaatcgc gttcaacacg
     4621 ctttgcgtgc ttttacgccc aaagatcaga aagcggtaaa agctgcggcg caaaccatgc
     4681 gggccaatcc ggcatttgat accgaaaagg caatccagga actggggacc ggcgaggcgt
     4741 taatctcgtt tcttgatgtg aaaggaagtc cttcagtggt ggaacgggcg atggtgattg
     4801 cgccttgttc gcggatggga ccggtgacgg aagatgagcg taatggcttg attaatcact
     4861 ctccggtgta tggcaagtac gaggatgagg tggaccggga atccgcctat gagatgttgc
     4921 aaaaaggctt tcaggccagt accgagcagc aaaataatcc ccccgcgaaa gggaaagagg
     4981 tagcggtgga tgacggcatt cttggtggat tgaaggatat tttgttcggc actaccggac
     5041 cacgcggcgg taagaaagat ggtgtggtgc aaacaatggc gaaaagcgcc gcccgccagg
     5101 tgacgaatca gattgtacgc gggatgttgg ggagtttgct gggggggaga agaaggtaag
     5161 tcaggtgcgg tctgtgcccc tcaccctaac cctctcccca aaggggcgag gggaccgatc
     5221 gaactcgtat ttgactttat caacaatgtg aaagggagct ttcgctccct tgcttggtta
     5281 cgattttctc attaacagcc acaggctgat taagaagaag ctggcgcttg gcaacagtgc
     5341 gccgatgatc ggcgggatgc cataaaccaa cgtcagcggg ccgaagatct ggtccagtac
     5401 gtagaagaca aaaccgaagc tgataccggt gaccacacgc acgcccatcg gtacgctacg
     5461 cagtgggcca aagatgaacg acagcgccat cagcatcatc accgccacgg atagcggctg
     5521 gaagattttg ctccacatgt tgagctgata acgtccggca tcctgaccgc tcgacttcag
     5581 atacttcacg tagttgtgca aaccgctgat tgagagtgca tccgggtcca gcgccaccac
     5641 gcccagtttg tctggcgtga ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt
     5701 aatctgtttc ggattggtca gatcagattc atcaacctgc gacagacgcc agactttatg
     5761 ttccgggtca aacttcgcag tagcggcata gcgtacggat tgcagacgac gattttcgtt
     5821 aaaggcataa atgctgatgc cacctaactc ttcgtcacct ttaacccgct caatgtagac
     5881 gaagttgttg ccatctttcg cccataagcc ttgctgggta gagagcaacg agccgccgta
     5941 catcgcctgc gcacggtagt tacgcgccat ctgctcgccc tgcggcgcga cccattcgcc
     6001 aatcgccatc gtcagcaaga ccagcggaat ggcggttttc atcaccgaca gcgccacctg
     6061 catacgggta aaaccagaag cctgcatcac caccagttcg ctgcgctgcg ccagcatccc
     6121 aagaccaagc aacgccccaa gcagagccgc catcgggaag aagatttgca catctttcgg
     6181 cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg taactcccct gcccggcttt
     6241 tttcaactga tcgacaaatt tgataatgcc cgacagcgac accagcatga acagcgtcat
     6301 catgatggtg gtgaaaatag ttttaccgat atagcggtca agtacgccaa aaggttgcat
     6361 cacaccgctc ctttacgcga aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa
     6421 aggttgagaa caatcgccaa agccagataa atcaggttaa cggtccacat ccacagcgtc
     6481 gggtccagct tacctttacc gccgttcgat ttcagggagg tctggatcag gaagaacaac
     6541 agatacagca gcatggctgg cagcatcgat agtacgcgtc cctgacgcgg gttaaccacg
     6601 ctcagcggta cgaccataag tgccatcata aacacggtga acaccaacgt gatacgccag
     6661 ttcagttctg cgcgagcacg atcggtgtca gtgttccaca atgtgcgcat gtccatctgg
     6721 tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa tgatcgcctg ataatcctgg
     6781 aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt cgaagcgcgt tccctggttg
     6841 agagtgacga cctgggagcc gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc
     6901 acagaaggtc gtgcattacc ttttggtcga atttgcgcga ggaacacatc tttgaaatcg
     6961 ctgccgtcaa cgctttcgat gaacagcacc gagctgccat tagtcgcttg ctggaattgc
     7021 ccttgcgcaa gcgccgccat gccagggttc gctttcgctt ctgctaacac ttcatcctga
     7081 tgacgcgatg accacggtcc cgcccacatc acgttaaccg ccgcgacgat tgccgtgaat
     7141 accgcaagga tcattgccgc tttcaccaga accgctttgc tcaggccgca ggcatgcatt
     7201 accgtaattt cactttcggt atacagtttg cccagcgtca tcagcagccc gaggaacagg
     7261 cttaatggca ggataagctg cgccatttcc ggcacgccca acccgagaag ggagagcacc
     7321 agattcgccg gaatatcgcc gtcaaccgct gcgccgagga tcctcactaa cttttgacag
     7381 aagaagatca aaagcaagat gaagagtatc gccagctggc ttttgagcgt ctcccgcacc
     7441 agatatctta tgattatcac tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt
     7501 agcttgtttc atggcttaaa cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga
     7561 taattatact caacggattc acctctcaga ttttgttctg acgtgccaat gccgtaataa
     7621 cgttaagatt aacacgaagt catcgcaaca gcggacatga gttacgaaag cttgcaattc
     7681 tatctgtagc caccgccgtt gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt
     7741 aaaaagcggt agcccggaga aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc
     7801 acgtcgcctt tctccgattg cagaacagct cgataaaatc agcgatgggt acatcagcgc
     7861 cctgctacgt cggggcgaac tggaaggaaa accggggcag acattgttgc tgcaccatgt
     7921 tccgaatgta ctttccgagc gaattctcct tattggttgc ggcaaagaac gtgagctgga
     7981 tgagcgtcag tacaagcagg ttattcagaa aaccattaat acgctgaatg atactggctc
     8041 aatggaagcg gtctgctttc tgactgaact gcacgttaaa ggccgtaaca actactggaa
     8101 agtgcgtcag gctgtcgaga cggcaaaaga aacgctctac agtttcgatc agctgaaaac
     8161 gaacaagagc gaaccgcgtc gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg
     8221 tgaactgacc agcggtgagc gcgcgatcca gcacggtctg gcgattgccg ccgggattaa
     8281 agcagcaaag gatcttggca atatgccgcc gaatatctgt aacgccgctt acctcgcttc
     8341 acaagcgcgc cagctggctg acagctacag caagaatgtt atcacccgcg ttatcggcga
     8401 acagcagatg aaagagctgg ggatgcattc ctatctggcg gtcggtcagg gttcgcagaa
     8461 cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat
     8521 cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt atctcgatca agccttcaga
     8581 aggcatggat gagatgaagt acgatatgtg cggcgcggcg gcggtttacg gcgtgatgcg
     8641 tatggtcgcg gagctacaac tgccgattaa tgttatcggc gtgctggcag gttgtgaaaa
     8701 catgccaggc ggtcgcgcct atcgtccggg cgatgtgtta accaccatgt ctggtcaaac
     8761 cgttgaagtg ctcaacaccg acgctgaagg ccgcctggta ctgtgcgacg tgttaactta
     8821 cgttgagcgt tttgagccgg aagcggtgat tgacgtggcg acgctgaccg gtgcctgcgt
     8881 gatcgcgctg ggtcatcaca ttactggtct gatggcgaac cacaatccgc tggcccatga
     8941 actgattgcc gcgtctgaac aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga
     9001 gtatcaggaa caactggagt ccaattttgc cgatatggcg aacattggcg gtcgtcctgg
     9061 tggggcgatt accgcaggtt gcttcctgtc acgctttacc cgtaagtaca actgggcgca
     9121 cctggatatc gccggtaccg cctggcgttc tggtaaagca aaaggcgcaa ccggccgtcc
     9181 ggtagcgttg ctggcacagt tcctgttaaa ccgcgctggg tttaacggcg aagagtaatt
     9241 gcgtcaggca aggctgttat tgccggatgc ggcgtgaacg ccttatccga cctacacagc
     9301 actgaactcg taggcctgat aagacgcgca agcgtcgcat caggcaatgc tgttattgtc
     9361 ggatgcggcg tgagcgcctt atccgaccta cacacagcac tgaactcgta ggcctgataa
     9421 gacacaacag cgtcgcatca ggcgctgcgg tgtatacctg atgcgtattt aaatccacca
     9481 caagaagccc catttatgaa aaacgcgacg ttctaccttc tggacaatga caccaccgtc
     9541 gatggcttaa gcgccgttga gcagctggtg tgtgaaattg ccgcagaacg ttggcgcagc
     9601 ggtaagcgcg tgctcatcgc ctgtgaagat gaaaagcagg cttaccggct ggatgaagcc
     9661 ctgtgggcgc gtccggcaga aagctttgtt ccgcataatt tagcgggaga aggaccgcgc
     9721 ggcggtgcgc cggtggagat cgcctggccg caaaaacgca gcagcagccc gcgcgatata
     9781 ttgattagtc tgcgaacaag ctttgcagat tttgccaccg ctttcacaga agtggtagac
     9841 ttcgttcctt atgaagattc tctgaaacaa ctggcgcgcg aacgctataa agcctaccgc
     9901 gtggctggtt tcaacctgaa tacggcaacc tggaaataat ggaaaagaca tacaacccac
     9961 aagatatcga acagccgctt tacgagcact gggaaaagca gggctacttt aagcctaatg
    10021 gcgatgaaag ccaggaaagt ttctgcatca tgatcccgcc gccgaacgtc accggcagtt
    10081 tgcatatggg tcacgccttc cagcaaacca tcatggatac catgatccgc tatcagcgca
    10141 tgcagggcaa aaacaccctg tggcaggtcg gtactgacca cgccgggatc gctacccaga
    10201 tggtcgttga gcgcaagatt gccgcagaag aaggtaaaac ccgtcacgac tacggccgcg
    10261 aagctttcat cgacaaaatt tgggaatgga aagcggaatc cggcggcacc attacccgtc
    10321 agatgcgccg tctcggcaac tccgtcgact gggagcgtga acgcttcacg atggacgaag
    10381 gcctgtccaa tgcggtgaaa gaagttttcg ttcgtctgta taaagaagac ctgatttacc
    10441 gtggcaaacg cctggtaaac tgggatccga aactgcgcac cgctatctct gacctggaag
    10501 tggaaaaccg cgaatcgaaa ggttcgatgt ggcacatccg ctatccgctg gctgacggcg
    10561 cgaaaaccgc agacggtaaa gattatctgg tggttgcgac tacccgtccg gaaaccctgt
    10621 tgggcgatac cggcgtggcc gttaacccgg aagatccgcg ttacaaagac ctgattggca
    10681 aatatgtcat tctaccgctg gttaaccgtc gtattccgat cgttggcgac gaacacgccg
    10741 acatggaaaa aggcaccggc tgcgtgaaaa tcactccggc gcacgacttt aacgactatg
    10801 aagtgggtaa acgtcacgcg ctgccgatga tcaacatcct gacctttgac ggcgatatcc
    10861 gtgaaagcgc ccaggtgttc gataccaaag gtaacgaatc tgacgtttat tccagcgaaa
    10921 tcccggcaga gttccagaaa ctcgagcgtt ttgctgcacg taaagcagtc gttgctgcgg
    10981 ttgacgcact cggcctgctg gaagaaatta aaccgcacga cctgaccgtt ccttacggcg
    11041 accgtggcgg cgtagttatc gaaccaatgc tgaccgacca gtggtacgtg cgtgccgatg
    11101 tactggcgaa accggcggtt gaagcggttg agaacggcga cattcagttc gtaccgaagc
    11161 agtacgaaaa catgtacttc tcctggatgc gcgatattca ggactggtgt atctctcgtc
    11221 agctgtggtg gggtcaccgt atccctgcat ggtacgacga agcaggtaac gtttatgttg
    11281 gccgcaacga agaagaagtg cgcaaggaaa ataacctcgg tgccgacgtt gccctgcgtc
    11341 aggacgaaga cgttctcgac acctggttct cttctgcact gtggaccttc tctacccttg
    11401 gctggccgga aaacaccgac gccctgcgtc agttccaccc aaccagcgtg atggtatccg
    11461 gcttcgacat catcttcttc tggattgccc gcatgatcat gatgaccatg cacttcatca
    11521 aagatgaaaa tggcaaaccg caggtgccgt tccacactgt ttacatgacc ggtctgattc
    11581 gtgacgacga aggccagaag atgtccaaat ccaagggtaa cgttatcgac ccgctggata
    11641 tggttgacgg tatttcgctg ccagaactgc tggaaaaacg taccggcaat atgatgcagc
    11701 cgcagctggc ggacaaaatc cgtaagcgca ccgagaaaca gttcccgaac ggcattgagc
    11761 cgcacggcac cgatgccctg cgcttcaccc tggcggcgct ggcgtctacc ggtcgtgaca
    11821 tcaactggga tatgaagcgc ctggaaggtt accgtaactt ctgtaacaag ctgtggaacg
    11881 ccagccgctt tgtgctgatg aacacagaag gtcaggattg cggctttaac ggcggcgaaa
    11941 tgacactgtc gctggcggac cgctggattc tggcagagtt caaccagacc atcaaagcgt
    12001 accgcgaagc gctggacagc ttccgcttcg atatcgctgc aggcattctg tatgagttca
    12061 cctggaacca gttctgtgac tggtatctgg agctgaccaa gccggtaatg aacggtggca
    12121 ccgaagccga actgcgcggt actcgccata cgctggtgac tgtactggaa ggtctgctgc
    12181 gcctcgcgca tccgatcatt ccgttcatca ccgaaaccat ctggcagcgt gtgaaagtac
    12241 tttgcggtat cactgccgac accatcatgc tgcagccgtt cccgcagtac gatgcatctc
    12301 aggttgatga agccgcactg gccgacaccg aatggctgaa acaggcgatc gttgcggtac
    12361 gtaacatccg tgcagaaatg aacatcgcgc cgggcaaacc gctggagctg ctgctgcgtg
    12421 gttgcagcgc ggatgcagaa cgtcgcgtaa atgaaaaccg tggcttcctg caaaccctgg
    12481 cgcgtctgga aagcatcacc gtgctgcctg ccgatgacaa aggtccggtt tccgttacga
    12541 agatcatcga cggcgcagag ctgctgatcc cgatggctgg cctcatcaac aaagaagatg
    12601 agctggcgcg tctggcgaaa gaagtggcga agatcgaagg cgaaatcagc cgtatcgaga
    12661 acaagctggc gaatgaaggc tttgtcgccc gcgcaccgga agcggtcatc gcgaaagagc
    12721 gtgagaagct ggaaggctat gcggaagcga aagcgaagct gattgaacag caggctgtta
    12781 tcgccgcgct gtaatcaaca ccgtgatgaa aacgaaggcc ggagaatgct ccggcctttt
    12841 ttatctctta cagaaatggt aaagaaggca taattccccg tgaaatactg gctaagaagc
    12901 ctttatctgg ttgttcttct ttattgatta aaggaaggtc atccaaatcg cccagcaaat
    12961 acgtattttt tgcctgccag tcaatcatcc atattttcag cagtggataa gccagaccgc
    13021 ccgtaatccc ggatatcact accaacgcac acatgcgata aagcataccg tggtaagtta
    13081 aagttgagcg aaaacgaata cgcccatcat tcagtgacag gttgctcata aaatgatttc
    13141 gcaacgacac cgttaaataa ctggtgctaa cagcaatccc aaaataatag attaactgcg
    13201 cgattatcat tttacgttgc atttccataa attgctgtaa attctcaata tcatcatttg
    13261 catacacaaa actatcatac gcatttaata tttgatcgaa gataatataa ccagcaacag
    13321 cgagaaaagg taataaagct aaaaatgcga gaatggcata ttttatacaa tacgtagtat
    13381 ctaatttcac cttgaaacga tgtataccga aactggtatt gctccagaga aaactcatta
    13441 ccagactata taaagtacca ttaaaaatac caatggaaac aataccaact attgccatca
    13501 gaaccacaga tataataaca ctacttgaac tattggcatg tagcatcttt gtcgagataa
    13561 agaaaacagt ccccatccca atggccatta aaatcggcaa gaaaaaggtt acccaccaga
    13621 accctttcaa agagcaatta aaactaaatc ttacgccgtt gagactggtc atcaaggcct
    13681 gataacgcag tccttttgct gccataaata taagcaaaac caacagtgac aaagtcaaaa
    13741 cacagccaac aagcggcata tctgctgaca ctg
//
LOCUS       DAZFZC010000117        13737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFZC010000117 DAZFZC010000000
VERSION     DAZFZC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.118"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            4..252
                     /locus_tag="ACHKXP_004181"
                     /pseudo
     CDS             4..252
                     /locus_tag="ACHKXP_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1307..1840
                     /locus_tag="ACHKXP_004182"
     CDS             1307..1840
                     /locus_tag="ACHKXP_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7649069.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2000..2137
                     /locus_tag="ACHKXP_004183"
     CDS             2000..2137
                     /locus_tag="ACHKXP_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649070.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            2359..2544
                     /locus_tag="ACHKXP_004184"
     CDS             2359..2544
                     /locus_tag="ACHKXP_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7649071.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            2783..3334
                     /locus_tag="ACHKXP_004185"
     CDS             2783..3334
                     /locus_tag="ACHKXP_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7649072.1"
                     /translation="MNYLTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            3627..4421
                     /locus_tag="ACHKXP_004186"
     CDS             3627..4421
                     /locus_tag="ACHKXP_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7649073.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLLPPSN
                     AFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGMFADL
                     EEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADRERIN
                     AARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            4402..6108
                     /locus_tag="ACHKXP_004187"
     CDS             4402..6108
                     /locus_tag="ACHKXP_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO7649074.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQMGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            6108..8252
                     /locus_tag="ACHKXP_004188"
     CDS             6108..8252
                     /locus_tag="ACHKXP_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO7649075.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPKWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QESGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            8410..9417
                     /locus_tag="ACHKXP_004189"
     CDS             8410..9417
                     /locus_tag="ACHKXP_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649076.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGIGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            9441..10655
                     /locus_tag="ACHKXP_004190"
     CDS             9441..10655
                     /locus_tag="ACHKXP_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO7649077.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            10711..11100
                     /locus_tag="ACHKXP_004191"
     CDS             10711..11100
                     /locus_tag="ACHKXP_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649078.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            11150..11611
                     /locus_tag="ACHKXP_004192"
     CDS             11150..11611
                     /locus_tag="ACHKXP_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649079.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAALAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            11595..12158
                     /locus_tag="ACHKXP_004193"
     CDS             11595..12158
                     /locus_tag="ACHKXP_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649080.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPSRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            12158..12808
                     /locus_tag="ACHKXP_004194"
     CDS             12158..12808
                     /locus_tag="ACHKXP_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO7649081.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            12805..>13737
                     /locus_tag="ACHKXP_004195"
     CDS             12805..>13737
                     /locus_tag="ACHKXP_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO7649082.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNAQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGL"
BASE COUNT         3591 a         3139 c         3884 g         3123 t
ORIGIN      
        1 acgatgaata aacaatacga actggttgtt aaagggataa atatttaccc ggataagatt
       61 actgttactg tggcgctgga aactggtggg tatacgtcac tgttgttgcc aaatgtggtg
      121 attgatcttg atcgtgttga aggtgccccg ctggagtttt acgaagctga ggcgaaaaag
      181 aaggcgaagc agtttttcat ggatattgct gccgggttat gtgaagggaa cgaaccgttg
      241 ccggaaaagc gcctctgttc agaagagcgg tataccatac aaattaacaa tgcatataac
      301 acgatacagt cagaaaaaga tgatatcgaa tcacgcattg aaaaactgga gaacagcgtc
      361 gttgaactga ataaaaaact gtcggtgcag atcccttccg aaggtgaaaa aaaacgccgt
      421 gatgagcagt tcgcggcgtt ttacgattat tgccggaaag tgatgagcag aaatctcgca
      481 gagtgtttcc gtattcaaaa aaacagagag cgctctgcaa atagtgagat tctggataat
      541 tttttaaagg ggacgcatct cgccagtgat tgtttcattg ctcattcttt tggtggcttt
      601 catgaggcca tcaaacatga tgttgagaga agtccaggta tcctctgttc cattcccgta
      661 agcatcgaaa tagacacaaa tacgataacc ggtgaactcg ttaccgccgg agtcggtata
      721 ttccgtcatg aggggaacga tgccaaaggt tcctttacaa aaatcgggcc gttctttctg
      781 aaggtagagc gatagtttgt taagtaccca tccgacagct cgctcatttt ggtttatttt
      841 cgtatcgcga aacaaaaatt cgaagcggcc agatgacgca tgggtgcaac caaataccgg
      901 ggcgtattca tcaacctttg gtgttcctga aaacatgcag tccgtgctct cgaatgcaga
      961 tgaattgctg ttgatccact taacaaaaga aaaaaaggtc tgaatcgctt gtctgtctga
     1021 atattcaaca aaatcctcaa ggtgtgtctc gatcagttcc gggtgtttac gaaagtcata
     1081 atattgaccg ggacgatatt tatgagtggt tgttttatcc catggttgtg ctctgcgtcc
     1141 ctgtaaacgg aggtctgccg ctggatagat aagcataagt aatctcctgg tggaatgtaa
     1201 tccacgatca tccggggaaa ttaagaaccc gccagtgccc accactggtg gggtgaaggc
     1261 ttaacatatc cagggattcg gaaccgataa atcctgataa atatccatga acaccaaaat
     1321 caaatacggc ctgtcggctg ccgttctggc gctgattgcc gcaggggcgc ctgcgcctga
     1381 tattctcgac cagtttctgg atgaaaaaga aggtaaccac accacggcat accgtgatgg
     1441 tgcgggtatc tggaccattt gtcgtggtgc caccagggtg gatggcaaac ctgtcgtccc
     1501 gggcatgaag ttgtcgaaga aaaaatgcga ccaggttaac gccattgagc gtgataaggc
     1561 gctggcatgg gtggagaaaa acatcaaagt gccactgact gaaccccaga aagcgggtat
     1621 tgcgtcattc tgtccttaca acattggccc cggtaagtgt ttcccgtcga cgttttataa
     1681 acgaattaat gcaggcgatc gcagaggagc gtgtgaagcg attcgctggt ggattaagga
     1741 cggtggcaga gactgccgta tccgctcaaa taactgttat ggtcaggtat cccgtcgtga
     1801 ccaggagagc gcgctggcgt gctggggaat cgacagataa gccgaatatt ttgctgaaaa
     1861 atgacgttgg ccaacgcgga cggataacac gaaatcctgc gaactggcaa aacctaagtg
     1921 aataaaagta aaaaccccgt ttgttggcag caagcggggt tttgtgtttc ctgacttcgg
     1981 aaaagtcaaa ggagaaagtg tgtttgattt tagcaaactg attcgggaga ttcgagtgat
     2041 ggctgaaaaa ttatccacct ggaagttcat ccttatctgg ctggtgtttg tgattatggc
     2101 ctccggttat ttcatcggtc agatacgctg gtggtgaaat gaaccgcgtt ctgtgtgtgg
     2161 tcatcattgc cctgctggtg gcctgtggtg cgcttagtct ggggctgaat cattaccgcg
     2221 ataacgccat cacctacaaa gcgcagcgcg ataaaaaagc cagagagctg gagcaggcaa
     2281 acgcaaccat tactgatatg caggtgcgcc agcgcgatgt tgctgcgctc gatgcaaaat
     2341 actcgaggga attagccgat gcgagagctg aaaatgaaac tctgcgtgct gatgttgccg
     2401 ctggtcgtaa gcgcctgcgg atcaacgcca cctgctccgg taccgtgcgt gaagccaccg
     2461 gcacctccgg cgtggataat gcaaccggcc cccgactggc agacaccgct gaacgggatt
     2521 atttcatcct cagagaacgg ttgatgacaa tgcagaagca gctggaaggg gcacaggaat
     2581 atatccgcac tcagtgcctg aattaacaga gccagcttaa tcgctggctt tttcatatct
     2641 gaatttcatc gcgcatctca cgcgcatatc acatccccga gcctttcaga aagttgagcc
     2701 tgagaactgc cgtatatggt ggcgaccatc tcggggcggc ttttctgtga gacaggctca
     2761 attttctgaa aggtaagacg ctatgaacta tctgacgatt gttaacggta ttgatttccg
     2821 cgatcttgtt tttgtgacag gcaacgagcc ggtgacggat acccgaaagg ttgccgtagc
     2881 atttggcaaa gaacacaaag atgtattgcg gaaaacaaga gccgttgttc aacaatgctc
     2941 aaaagaattt gcagagcgca attttgcgct ttgctatgaa aacaatgagt tacagaacgg
     3001 taaaccccag cccatatatc gaatgacaaa agacggttgg acgatgttgg tatttggttt
     3061 tacggggaaa gctgctatga cctttaagga agcgtacatt caggctttca actggatggc
     3121 tgagcttatt cagcaaggtc tggcgaatct ggaggcggag cgcaacgctg taatgctgga
     3181 gtacatgaaa gagaaagatg tcgccagtat gtcaggtcgt ttgctcaatc gctggggcag
     3241 ggtgaagaag cctcagttgc tggcaaggct ggacaggctg gagcagcagg ggcagattgc
     3301 gttacccgga tttgataaag gcatttcagc ctgacatagc catgcgccgt atcgtcgccg
     3361 tattcccgca ttaacagaga ccgcagccac cttatctgcg tgagtgtgcg gggataatca
     3421 aaaacgatac acaccgggtt ttctcatttt tcacgagatg ggagcgattt cccgcgaagc
     3481 cgcctgtccg gtgcggtggt ggaagaaacc ggataaaaca accgcattgt gcaaatatcg
     3541 atcaaatatg gtgctgctgt gtgaaatctg aaaaatcaca gcggtcatta tgcatcagtt
     3601 tttaacacag gacgtcagaa cgtgacatgg caaagctgga ctggaaaaag ctggagcagg
     3661 cattccgacg cgaacatgcc gaaacgggaa taacattact ggactggtgc cggaagaaaa
     3721 agattaatta caacaccgcc agaacccgta taaaaatggg caaaatcgat catgaaattg
     3781 atcataaaac cgatcatgac atctcagatg aagaaccctg caatgacgcg ggttccggcg
     3841 atgaaaaatg cgcaaaaaac tctgaaaaaa actgcgcaaa ttcggcagaa acgaaacgga
     3901 ttcgtggttc ccgactttta ccaccttcaa acgctttttc tcagcgaaac acccacgccg
     3961 taagacaccg tggatatgcg aagtatcttg aggcagataa cctcatggat gatgcgtccg
     4021 acatggtgct gttcgatgaa ctggtgttca cccgggcccg cgcactttca gtaactaagg
     4081 cacttaaagg gatgttcgcc gacctggaag aggcaactga cgtggaaacc cgtgttgctc
     4141 tttacgacaa aatactcaaa gctgaacagg cccttgaccg gaatattgcc cgtatcgagt
     4201 caattgaacg ctcattgctg acgctggacg tcctagctga gacagcacca aaacttcgtg
     4261 ctgaccggga aagaatcaac gccgccagag ataaactcag agctgaaacc gatattctga
     4321 ccagccagcg tcggggcgtt gttacgcctg tcagtgacat cgtgtcatcg ctgcatgaaa
     4381 tgagtaattc ggggagactg gatgacattc cggaagaatg aaccgcgatg tgatgagccg
     4441 tcagaaatga ccgaggctga acaacgtctg ttcatcatga ctaaactgag caatccctgg
     4501 tggcggctca atcatctcta caaaatacag aacgaaaaag gtgaactggt caccttcaga
     4561 atgcgaccgg cgcagcgcca gttgttccgg agcatgcaca ataaaaatat tatcctgaaa
     4621 gcgcgccaga tgggattttc cacagccatt gatatttatc ttctcgacca ggcattattc
     4681 attccgcatc tcaaatgcgg gatcgtcgct caggataaac aggctgccag tgaaattttc
     4741 cgcacaaaaa ttgctgtacc gtttgatcat ctccctgact ggctgagagc ctcattcacc
     4801 atcgttgaac gtcgtagcgg tgccagcggt ggctatatcc tgtttggtca cggctcgagt
     4861 atccaggtgg caacctcatt ccgttcaggt acggtgcagc gcctgcatat ctcagagcac
     4921 ggcaaaattt gcgcgaaata tccggctaag gcgaaagaac tgcgaaccgg tacgcttaat
     4981 gccgtctctg atgaatgcat tatttttgat gagtccactg ctgaaggcgt gggtggtgat
     5041 ttttacgaga tgagtaaccg agcacaggag atcactgcat caggcttatt gctgacggca
     5101 caggattata aattccattt ttacgcctgg tggcaggatc ctaaatacag cgccagagtg
     5161 ccggaaagcg ggctgaagct gtcacgggaa aaaatgacgt atttttctgc ggttgagaag
     5221 gcaatgaaca tcacgcttac tgatgaacag aagcagtggt acatcaataa ggaaactgaa
     5281 cagcgtgagg aaatgaagca ggagtttccc tcaacgccac aggaggcgtt tctgacgtcc
     5341 ggacgacgtg tgttcagtgc cgaaagtacg ttgcaggcag aatcattctg ttcgccaccg
     5401 atgattgttt atgacattga acctgttaca ggagcgaaga ctaaagctca gtctctgcgt
     5461 gaaggaaata aaaacgagtt gcagcggacg ctgatgaatt atctgctggt atgggaactg
     5521 ccggatccgg atgaagagta tgtttgtggg gcagatactg ccgaagggct ggagcacgga
     5581 gaccgctcat cgctggatgt tgtcaaacgc agtaatggcg agcaggtggc tcactggttc
     5641 gggcatctcg atgctgaact ttttgctcat ctcatttcgc aggtctgtcg tatgtataac
     5701 aacgcgtttg tggggccgga gcgtaataat cacggacatg cagttatcct gaaactccgg
     5761 gaactctatc cgacacgtta tatctacaac gaacagcatc ttgaccaggc atatgacgac
     5821 gatacgcccc gccttggctg gctgacaacc cgtcagagca aacctgttct gaccgaagga
     5881 atgaaaacgc ttctgaataa tggaatatca gggatccgct ggtcaggcac attatcggaa
     5941 atgaacacct acgtttatga cgcgaaaggc tccatgaatg cacaggaagg ctgctttgat
     6001 gatcagctca tgagctacat gattgcccag gagatgcgcg ccagaatgcc ggtgagggta
     6061 aaacagaaaa cggataaacg cagaaccaca cactggatgg cacactgatg aaaaatgaaa
     6121 ttaacaccac agcgatgaaa aacgatcatg gatccacgcc gcgtttttct cagcgtcagt
     6181 tactgtctct ctgttctgat attgacagcc agcctaaatg gcgtgatgcc gcaaacaagg
     6241 cctgtgcgta ttatgatggc gaccagctgg caccggaagt tatccaggta ctgaaagatc
     6301 gcggtcagcc catgaccatc cataacctca ttgcccccac ggtagatggt gtactgggaa
     6361 tggaggcaaa aacaagaacg gacctgatag tgatgtcaga cgatccgaac gatgaaacag
     6421 agaaactggc agaggccatt aatgcggagt ttgctgatgc gtgccgtctt ggcaacatga
     6481 ataaggcccg ctccgatgcc tatgcggaac aaatcaaggc gggcctcagt tgggtggagg
     6541 tcagacgaaa cagtgatccg ttcgggcctg aatttaaggt gtctactgtc agccggaatg
     6601 aggttttctg ggactggctg agccgggagg ctgatttaag tgactgccgc tggctgatgc
     6661 gtcgccgctg gatggatacc gatgaggcaa aagctacatt cccgggaatg gctcaggtta
     6721 tcgattatgc cattgatgac tggcgtggtt ttgtcgatac cacggttact gaaggccagc
     6781 ccagtccgtt gatgagtgca tgggaagagt atcagtcatg ggatcgacag cagaacgaat
     6841 ggcttcagcg tgaacgccgt cgtgtgctgc ttcaggtggt ttattaccgt acattcgagc
     6901 gtcttccggt gattgaactc agtaatggac gggtggtggc ctttgataaa aataatctga
     6961 tgcaggcggt agctgtggca tccgggcggg ttcaggtgaa agtcgggcgg gtaagccgta
     7021 ttcgtgaagc ctggtttgtc gggccacact ttattgtgga tcgcccctgt agtgctccgc
     7081 aggggatgtt tccgctggtt cctttctggg gataccgaaa ggataaaacc ggggagccat
     7141 acgggctaat ttcccgcgcc attccggcac aggatgaggt gaattttcgt cgtatcaagc
     7201 tgacctggtt gcttcaggcc aaacgcgtga ttatggacga ggatgccacc cagttgtcag
     7261 acaacgacct gatggagcag atcgaacgtc cggatggcat tattaaactg aatccggtcc
     7321 gaaaaaatca gaaaagtgtc gcagatgttt ttcgggttga gcaggatttt caggttgcca
     7381 gccagcagtt tcaggtcatg caggaatcgg aaaaacttat ccaggatacc atgggagtgt
     7441 attccgcatt tctcgggcag gagtcaggtg cgacgtcagg cgtggctatc agtaacctgg
     7501 tggagcaggg ggccacaacc cttgcggaaa tcaacgataa ctaccagttt gcctgccagc
     7561 aggtgggaag actgttgctg gcttatcttc tcgatgacct gaaaaaacgc cgtaatcatg
     7621 cagtggtgat taatcgcgat gatcgccagc gtcgccagac cattgtcctc aatgctgaag
     7681 gtgataatgg tgaactgacc aatgatattt caagattaaa tacacatatt gcgctggcgc
     7741 ctgttcagca gacaccggcg tttaaggcac agcttgcaca gagaatgtca gaggttattc
     7801 aggggctgcc gcctcaggtg caggctgttg tgctcgacct gtgggttaat cttctggatg
     7861 tgccgcagaa acaggagttt gttgagcgta ttcgtgctgc gctggggacg ccaaaatcac
     7921 cagatgaaat gacgccggaa gaacaggaag tagcggcaca acaacaggca cttcagcaac
     7981 aacaggcaga actccagatg cgcgagatgg ctggcagagt ggcaaaactg gaagctgacg
     8041 ccgccagggc acatgcagct gcacaacggg ataatgccag tgcacagcgg gaagtcgccc
     8101 tgacacaggg gcagcgttat gtggatgcgc ttaaccaggc acatacggca gaaatcatta
     8161 ccggcgtaca gaatatggaa caggagcagg acgttcttca gcaacagatg ctgtatacgt
     8221 tacaacagcg gatgaatgaa atgtcgctct gaaaactctg gcttcaactg aaccccgtca
     8281 tcgtacgggg ttttttgttt ccggaggtaa gcgttccggg agcggtgcgc ttattcgcgg
     8341 gggcagcgat aagccttatt tactcaacca ttcggatctg tccgataaac agaccatgcg
     8401 gagttattta tggattttga atttacgggt gaagaaaccc cggaacaact ggaaaaaatg
     8461 ctggaaggac ttggggatgt ggatattgac agtcacgcac aggacgtcgt gacggaagat
     8521 accacggaaa aacatgcgga tgaggaagca cagactcaga cgggcgataa caatgtggca
     8581 ccgacgccgg atgccagtgt ggagcagacg caggacgtga aggagccgga agcgaagggg
     8641 gtgctcaccc gcgacggtaa acacgttatt ccctatgaag tccttgaggc tgaacgttcc
     8701 ggtaagcaac gggccgaaca ggaagccgca cttcttcgtg ggcagatagc tgaagaaaaa
     8761 cgcagggtgg aactgctgac gtctcagatc caccaggccg gtatgaagcc cacaccgtta
     8821 ccggaaaacg aaaaaatttc tgatgagcag attgcccgta tcagggagat gtatccggaa
     8881 attggtgacg cggtggcttc gctcatccgt aaaaataact atctccagtc ccgtgttcag
     8941 caatcaacac agcaggcaga aggtaatggt ggtgaggatt tatcaccggt tcttgatgcg
     9001 atgaatgccg tgccggtgct gaaaacgtgg caggagtccg atccggatcg cttctcggtt
     9061 gccgtatcca tcgacgggaa gctccagaat gaccccgcat ggaaagacaa aacgctcact
     9121 gaacgtttcg ctgaagtggc ccgtcgtacg caggttgctt tcggtgaagt cagtgagtcg
     9181 tctgctgaca acaaggcaga caaaacggat atccggaaaa cggcggaaga gaaagtgaag
     9241 accgctgaac aggagcaggc agtacctgct tccccgtcag atttaggcac cacggcctcc
     9301 gtcggaatcg gtgataattt tgaacggtta cttggtgctt ctcattcaga ggcagaggcg
     9361 attatgcgtg gtatgacgaa tgctgaaata gacgcacttc tggagaagct agggtaactt
     9421 actgaaggag tactgaagta atgacgactg taacatcagc ccaggcgaat aagctgtatc
     9481 aggtggcgct ttttactgct gccaaccgca accgctcgat ggttaatatc cttactgaac
     9541 agcaggaagc gccaaaagcg gtttcgccgg acaagaaaag cacgaagcag accagcgcag
     9601 gtgcgccggt tgtccgtatc acagacctta acaaacaggc cggtgatgaa gtgaccttca
     9661 gcatcatgca caaactctca aaacgtccga cgatgggaga tgagcgtgtt gaaggtcgtg
     9721 gtgaggatct cagccatgct gacttctccc tgaaaatcaa tcagggacgt cacctggtgg
     9781 atgcaggcgg acgtatgagt cagcagcgca cgaagtttaa cctggcatcc tcagccagaa
     9841 cgcttctggg gacgtacttt aatgacctgc aggaccagtg tgcgatagtg catcttgctg
     9901 gagctcgtgg tgattttgtt gctgacgaca ctattctgcc gacagcggag caccctgaat
     9961 tcaaaaaaat catgatcaac gatgtactgc ctccgacaca tgaccgtcac ttttttggcg
    10021 gtgatgcgac aagctttgag cagattgaag cggcagatat tttttctatt ggcctggtgg
    10081 acaatctctc cctgttcatt gacgaaatgg cgcatccgtt acagccggtt cgtctgtccg
    10141 gtgatgaact tcacggagaa gatccatatt acgtcctgta cgtcacgccg cgtcagtgga
    10201 atgactggta cacctcgacg tccggtaagg actggaacca gatgatggtt cgtgccgtga
    10261 accgtgcaaa aggttttaat catccgctgt tcaaaggtga atgtgcgatg tggcgcaata
    10321 tcctggttcg taagtatgcg ggtatgccga tccgtttcta tcaggggtca aaggttctgg
    10381 tatcagagaa taacctgacg gcaaccacga aagaggtcgc tgctgcaacc aatattgacc
    10441 gcgccatgtt actgggggct caggcgctgg caaatgctta cggtcagaag gcgggcggtc
    10501 acttcaacat ggttgagaag aaaacggata tggataaccg tactgagata gcaatcagct
    10561 ggatcaacgg tctgaaaaaa atccgtttcc ccgagaagag cggcaagatg caggatcacg
    10621 gcgtgattgc cgttgataca gcagtgaagc tctgattttt tcctttccct atgccgggtt
    10681 ttcgcccggc tttttcagga gtcattaatt atggcaaaga ctatccttgc cccgtcactg
    10741 agtgaacggg tctatacggg tacgcacggt aatgagtcgg tggcagaagg cgtatttacg
    10801 gtgaatgctg cggaagcgga cagtgttatt catcttctct cactgccagt gggcatccgt
    10861 atcaactcac tccaactggt ttcaacgggt ggtctgggta ctgcaaccgt cagcattaag
    10921 tccggtgagc atgctctcat cgataacagc gaagctgttt ctgcaaaatt tgccagatat
    10981 gtgccagtgg agccgtacac cacacagcgt gacggggagc tggttactgt caccattaag
    11041 actgccgctg caaccggcac cctgaatgtt ctgctgcgtt ataccgtggt gggatactga
    11101 ttaaaacctt ccggcccgcg tcatgcgggc tttttatccg gggaattata tgagtgagaa
    11161 aattgccgtt gtctatatcg gcccaaaacc cgtgaaaaag gacaccatta ccggaagtcg
    11221 cacgctgttc ccacgtcttg agccggtgca tgttgacagc gcgatggcct ggcaactgct
    11281 ggggtttccg gatgtctggg ttcgtcatga agagcttgat gatgttctga aaaagcaaca
    11341 acagaatgag cagttgcggc aggcacagca ggcgcaggaa agagtgcttg ctgcgctggc
    11401 agaagcggag aacagttttg ttgtttctgt taacgggcag gaggtggatt taagtaagct
    11461 cacctcagca cggctggcga cgctgtgtga ggcagaagag ctggatattc acaaagaccc
    11521 gaaagaaacg gctgaggcat tccgtatccg ggtgcgtgag gcatttcgcc gtcgtgttgc
    11581 ggagactgaa cagcatggcg gaactgagtg attttttacc gtatgtccgt cgtcatatca
    11641 gcggtccact gaacattatg atgacggatg ctctgtcaat ggctgccgtg gcattcagcc
    11701 gccagtcgtt ggtgtgccgt cgggaggtta ctgttgtacc agtagcagga aaagaaatcg
    11761 tgcttccgta tgacaaagat gatgaggagt gcgttcatat catccgtatc tctgacgata
    11821 atcatgagct ttttgtcggt cgggatgtgg atatcagctc cggacgctcc ctgcgatttg
    11881 cctgttctcc cggtgaggtg agcgtgcttt atgccgtcgc tccgaaagcc ggacgcagcc
    11941 agataccgga tgaactcctc acatggcctg aagaagtggc tgcgggggca cttgagcggt
    12001 tgttcatgca gactggtgtt tcatggtcag atccgtcacg cgcacagtat ttttctgtgc
    12061 agttttctga ggggatccgt cgggcatatc gtcatacact ggcgacaagc ccgtactctt
    12121 cataccgcaa ccctgtacgc aggcagaggt tttactgatg acgacgatta cagaaatcat
    12181 cggacgtgtg aatacacaac tggttgaccc gatgatggtt cgctggcccc tgcaggaatt
    12241 gtgcgattat tacaatgacg ctgtgagggc agtgattctg gcgagaccgg atgctggcgc
    12301 aagcctggaa acaatcagtt gtgttcctgg cgcccgtcag gttttgcccg atggtgtaat
    12361 acaacttctt gacgtgatat gcctcagtga cggtagtgca gtcagaccat tatcccggga
    12421 ggtgctggat gcgcagtatc ctgagtggcc cacaatgaag ggtattcctg aatgttttat
    12481 cagcaacgac ctgtccccgc gcgtattctg gctgtttcct gctcctgaca aagagataag
    12541 tattgatgca gtggtaagcc ggataccgga ggcagtgtat gttctgacgc aggacgatga
    12601 tacgccagtt ccactggaag aggcttatgt taacccactg gtggactgga tgttgtttcg
    12661 cgctttcagt aaggatgctg ccggtggcgc agaatcgggg ctggctgcgc agcattatca
    12721 gagttttgtt gagcaactag ggatcaaaca gggggcagac agtgcattgt ctgcccgtaa
    12781 aaaagtgttt aacggaggtg gagtgtgagt gttgttgttt cggggacgct gaaatctcct
    12841 gatggtgagg cgatatcagg agcaaatatt accctgacgg cgctgacagt ttcaccggat
    12901 gcgctcagcg gcaccagtgc gtcggcagtg acccgtgaag gtggatatta cggaatgacg
    12961 atggatccgg gggagtatgc ggtttcggtg acggtgaaag ggaagactgc tgtctacgga
    13021 cgtgtgcgta ttgaggggac cgaaagtacg gtgacgctca atatgctgtt acgccgcagt
    13081 cttgttgagg ttagcatacc cggagaactg ctgacagatt tccggcagat acagaataat
    13141 gtggctgatg accttgccac tattcgtcgc ctgaatgaag acacggcgac aaaaaacgct
    13201 caggccacac agtcaaaaga aagtgcagca gccagtgcga agagcgcatc tgacagtgca
    13261 aagacggcaa ccagcagggc ggctgaagcc ggacaaaaag cgactgatgc cactgaggct
    13321 gcgacccgtg cagtcacagc agcggggaat gcagaggaaa gctcgacccg tgccggagag
    13381 tctgaaaaag ccgccggagc tgatgcagaa aaagccagac agcatgctga aaaggccagg
    13441 ctggcgcagg agagcgccgg agagatcctt aagcgggcag aggctgccac tgtcagtgct
    13501 gaagaggcca gacgtatggc tgagaatgca cgggggcccc gggggcctca gggagaaact
    13561 ggtccgaagg gggatgtcgg tcctaaaggc gaaacaggtc cagtgggccc tcaagggccc
    13621 gcagggccga aaggtgagcg tggtgacgtt ggtgctcagg gggctgtagg gcccgctggt
    13681 ccgcgtggtg agaagggcga acagggggag cgaggaccgc agggaatacc aggcctg
//
LOCUS       DAZFZC010000118        13358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFZC010000118 DAZFZC010000000
VERSION     DAZFZC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.119"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKXP_004196"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKXP_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO7649083.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHKXP_004197"
     CDS             516..1217
                     /locus_tag="ACHKXP_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO7649084.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKXP_004198"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKXP_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO7649085.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1538..2848)
                     /gene="cyuA"
                     /locus_tag="ACHKXP_004199"
     CDS             complement(1538..2848)
                     /gene="cyuA"
                     /locus_tag="ACHKXP_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO7649086.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACMAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2876..4207)
                     /locus_tag="ACHKXP_004200"
     CDS             complement(2876..4207)
                     /locus_tag="ACHKXP_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7649087.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4482..5846)
                     /gene="tdcG"
                     /locus_tag="ACHKXP_004201"
     CDS             complement(4482..5846)
                     /gene="tdcG"
                     /locus_tag="ACHKXP_004201"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7649088.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5918..6307)
                     /locus_tag="ACHKXP_004202"
     CDS             complement(5918..6307)
                     /locus_tag="ACHKXP_004202"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO7649089.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6321..8615)
                     /gene="tdcE"
                     /locus_tag="ACHKXP_004203"
     CDS             complement(6321..8615)
                     /gene="tdcE"
                     /locus_tag="ACHKXP_004203"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO7649090.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8649..9857)
                     /gene="tdcD"
                     /locus_tag="ACHKXP_004204"
     CDS             complement(8649..9857)
                     /gene="tdcD"
                     /locus_tag="ACHKXP_004204"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO7649091.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGLEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9883..11214)
                     /gene="tdcC"
                     /locus_tag="ACHKXP_004205"
     CDS             complement(9883..11214)
                     /gene="tdcC"
                     /locus_tag="ACHKXP_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO7649092.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11236..12225)
                     /gene="tdcB"
                     /locus_tag="ACHKXP_004206"
     CDS             complement(11236..12225)
                     /gene="tdcB"
                     /locus_tag="ACHKXP_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO7649093.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12324..13262)
                     /gene="tdcA"
                     /locus_tag="ACHKXP_004207"
     CDS             complement(12324..13262)
                     /gene="tdcA"
                     /locus_tag="ACHKXP_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO7649094.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3464 a         3592 c         3153 g         3149 t
ORIGIN      
        1 cgcccccgcc agcacttctg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gagcttccgc atcagtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgctcca gcaacatcct cccgacattg gtgaatttgg tacgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgatagcg aaggcaatca tgttcaggcc agcgccggag
      781 aggcgttgct gctctctacc cagccgggtg tcagctatag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacgcgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa gctggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggtg
      961 cgatgggaag cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa attggccaaa
     1261 aagcgccagc cggtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gctatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc
     1441 ggcctacaaa atccaacaac attaacgaat taaacaaccc gtaggccgga caagatgcgc
     1501 cagcatcgca tccggcacga tccccaaaac ctggcgttta tctggccttg ctcgccataa
     1561 tctcgataat ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg
     1621 caatcgactg ctcaacatca tgcgccacaa tcccttcatt gccggtcacg gcggtatcat
     1681 ccagcgccat taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc
     1741 agctgttcga cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg
     1801 ccatcgagat ggtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca
     1861 ttgctgcggt cgttgcggca cacagcgcag acaaacgcgg taactggtta tggatgtaaa
     1921 ttgcgctcaa atgcgaaagc atcagcgcac gcgccagccg ttcatcatcc gctccgaagt
     1981 gttctgctac caccaccaca ggcattgttg cggtaatccc ctggttaccc gagccggagt
     2041 tactcatagc cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa
     2101 tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg
     2161 tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta
     2221 gcttcgcgga atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca
     2281 ggatctcagc cagcgtcgtt ctggaaagca ccgtcagcgg agactcttgc tcgccctctg
     2341 ccatacacgc ctgctgggta aacaccacac catcgtgcgt ctcgatatgc acaatgttgg
     2401 tatgcccgcc gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg
     2461 agaagaggat ttcatcgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca
     2521 gtgctttggc atcggcaatt gcctgcgctg ttgcgtcttt cagcacttcc agcccggcgt
     2581 tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc
     2641 cgggaacggt gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac
     2701 gttcaaccgg accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg
     2761 gttcagtaca tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc
     2821 ataacggatt taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag
     2881 aaaatgccag gaacggagaa acacacagca acaaaccagt gacgataatc aggtacagag
     2941 acatcccttt gtatttgtgc aatgctggta ctttgtaaac cagccacgcc gggatcaggc
     3001 accctaccat gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca
     3061 gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacgagat
     3121 tttcgttaat cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg
     3181 ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga
     3241 gaatgacgct gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa
     3301 tcgccagcgc ggaaatattc tgctcatagg cttttaccgc ttcgtcatgt cccatcgcca
     3361 gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca
     3421 ttgcccgcaa tgctttatgc cgcgccactt caattgattt ttcccgcgag cgataagaga
     3481 tcaccatcgg acttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa
     3541 taatggcgtt tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc
     3601 acattcccac catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc
     3661 cggtcgaaat tttgaataac aatttctcgc cgcgtgagga gatcgccacc agaatgcaaa
     3721 tcagtaccag accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat
     3781 gcaggtagga agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca
     3841 gcattacgaa atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa
     3901 tgacgctcgg gtaatcttta cattctggtg attctgccag cgtattaata aacaatcgct
     3961 gaaacagata cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca
     4021 tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca
     4081 taatcaccca gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac
     4141 tcattccagc acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa
     4201 tttccataat ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca
     4261 aggcgatatg cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc
     4321 gaggtcagag aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt
     4381 ctactaaatt aatcgcagga gaatattcat tctctatttg cggcagatca caaaaaaaag
     4441 gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta
     4501 atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca
     4561 tacatcgtct cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc
     4621 atccgcgcgg cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt
     4681 tgtacctgtc cggcaaccgg atcgcaggtc agtccaaggt tatgctccat cgcgatttcc
     4741 gccgcattgc atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc
     4801 attgaacagg ccacgccaat ctccccctga cagccgactt ccgcgccaga gatggaggcg
     4861 ttcattttat acagcgcgcc aatagccccc gcggccagaa aatagcgggc aattgaccgc
     4921 tcgtttaccg gacgacggaa cttatcgtaa taagccagta ctgccggaat aatgccgcac
     4981 gcaccgttag tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc
     5041 agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgctg
     5101 gaaaccagct gacgacgcag cgctacggca cggcgcggca cattgagcgg accaggcagc
     5161 acgccttcag tgttcatccc acgttcaata ccgtcatgca tcacttgcca gatacgggca
     5221 aaaccggcgt caatttccgc tttgctgcgc agcgctagct cgttgtgcat catcagacca
     5281 gatatagaca ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg
     5341 taaggtacgg acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac
     5401 ccgccgccga cagagtaata ggttttactt aatagctctt cctgtccctt ccaggcagtg
     5461 atccgcattc cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct
     5521 acaggaaaat caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact
     5581 aactctataa atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt
     5641 cccatgatga tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac
     5701 agatcgacca caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata
     5761 aaactttttc cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt
     5821 ttgaaaatat cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg
     5881 agcggaacga ccttacgacc gtcccgctca cgaggcttta cgcactacgt actgcgatgg
     5941 cttcaatttc cagcttcaca tctttcggca aacgcgcgac ctgcacacag ctccgggtcg
     6001 gataggtcgc ctgatgctca tcgaagaact gcttatagac ttcgttgatg gtggcaaaat
     6061 cattcagatc ggtgataaac acggtcatct tgatgatatc gcccacgctc agcccggcag
     6121 caaccacgat cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg
     6181 ggatctcacc ggtctgtggg caaaccggta tttgcccgga ggtgaagacc atgctgccta
     6241 aatcaacgcc ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct
     6301 ttttcataca tcctccggcg tcagagcgcc tgggtaaagg tacgtgaaat aacatcctgt
     6361 tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg
     6421 ttaggatatt tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg
     6481 acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg
     6541 ccgacaaggt tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag
     6601 aaggtgtacg agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat
     6661 gaggccacgg cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt
     6721 gttccggcgc gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa
     6781 gtgatagtca gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt
     6841 ttcttcataa agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg
     6901 ccgtactgcg gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg
     6961 attggtttca cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc
     7021 gcgatgccgc atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct
     7081 tcgtagctgt acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc
     7141 gccagccagt ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg
     7201 tccatcagcg gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg
     7261 ttaattgcgt agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc
     7321 ttaccaatca ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca
     7381 gtacgcatca gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg
     7441 gcatattttt tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc
     7501 ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc
     7561 gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag
     7621 aacagcgaat caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag
     7681 tgatcgatca gttcctgtgc ctgctgctca ttgagtacgc cagctttaaa gtcgcgctca
     7741 atgtagatat cgaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat
     7801 ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc
     7861 gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga
     7921 cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc
     7981 agacgagact ggagatcggc aaattgcagt tcgcgttcac gtaccagata actgatgcca
     8041 tacagcgcta cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa
     8101 ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg
     8161 ccctggttat gggttttacg cagatcggta aacagatatt caaattcact gtccatttct
     8221 cggccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca
     8281 cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat
     8341 cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg
     8401 gttgcatttt cgatacggat gccttccatt actttttccc acaattccgt ggtggcaggc
     8461 gtcgcttcgg cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa
     8521 tcgcggacat taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg
     8581 tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt
     8641 caactaaatt atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa
     8701 gcaatcattt tttcttcgtt agtcggaata acggcacaaa tgacacgcgc attttcactg
     8761 gaaacaattc gctcaccaca ggagttagag cgattattca tttctgtatc aatctctaag
     8821 cctaatacag ccaaatgttc catgaccaga cgacgaatta agcttgaatt ctctcctatt
     8881 ccgccggtga atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga
     8941 cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc
     9001 caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac
     9061 tctttattca ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag
     9121 gacatcgcac caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc
     9181 gtcattccca ttgaggtatc aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca
     9241 ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt
     9301 gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac
     9361 tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg
     9421 aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca
     9481 ataccactta aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta
     9541 tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg
     9601 cggtggccaa ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt
     9661 gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg
     9721 gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag
     9781 tcgctggcat cgagcacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca
     9841 accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac
     9901 gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg
     9961 gtatttcgcc agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga
    10021 tgcgataatc ggtgcgccca tggcttcaat caggtcaagg atgttcgggt tggcgtaggc
    10081 aacaacccag gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga
    10141 cactttagtt ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag
    10201 cgttcccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc
    10261 cgcatattcc agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt
    10321 agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga
    10381 cagagtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc
    10441 acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata
    10501 ctcttcacgc ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac
    10561 catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag
    10621 cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca gcgacaaaga
    10681 aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag
    10741 atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc
    10801 gcgattcagc ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat
    10861 agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac
    10921 cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt
    10981 agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaacgcga tggggtatgc
    11041 caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa
    11101 cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt
    11161 acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta
    11221 tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc
    11281 gatattgccg ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt
    11341 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
    11401 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
    11461 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
    11521 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
    11581 gccgtgaacg ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
    11641 tgccaccgca ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac
    11701 atcatagaga tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg
    11761 atcatcgtaa ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc
    11821 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
    11881 cgctactttg gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat
    11941 cgcgcaggag agggaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
    12001 gcgtttttcc gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
    12061 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
    12121 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
    12181 aataatgtca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
    12241 cctgaattaa atatgtaggt aaccgtaacc gacacctgca agacagacag gtggattatt
    12301 tatgtttaaa taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca
    12361 ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga
    12421 taatttttcg accataccgc agcatattgt gccacaggta atgtttcttc aaccggaata
    12481 gtaataaatt gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa
    12541 tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca
    12601 atactgatgc catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt
    12661 tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt
    12721 cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc
    12781 tgaagcttca tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt
    12841 gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc
    12901 tctttgaact tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca
    12961 aatgagactt ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc
    13021 atttcacggg taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt
    13081 acaccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg
    13141 attttactga cggccggttg agttaaccct aattcttttg cagccgagcc gatagaacca
    13201 cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc
    13261 ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca
    13321 ggatatgtgc gaccactcac aaattaactt tcaatact
//
LOCUS       DAZFZC010000119        13125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFZC010000119 DAZFZC010000000
VERSION     DAZFZC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.120"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(311..1594)
                     /gene="gltA"
                     /locus_tag="ACHKXP_004208"
     CDS             complement(311..1594)
                     /gene="gltA"
                     /locus_tag="ACHKXP_004208"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO7649095.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1800..1907)
                     /locus_tag="ACHKXP_004209"
     CDS             complement(1800..1907)
                     /locus_tag="ACHKXP_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649096.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2288..2692
                     /gene="sdhC"
                     /locus_tag="ACHKXP_004210"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2288..2692
                     /gene="sdhC"
                     /locus_tag="ACHKXP_004210"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7649097.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2686..3033
                     /gene="sdhD"
                     /locus_tag="ACHKXP_004211"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2686..3033
                     /gene="sdhD"
                     /locus_tag="ACHKXP_004211"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO7649098.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            3033..4799
                     /gene="sdhA"
                     /locus_tag="ACHKXP_004212"
     CDS             3033..4799
                     /gene="sdhA"
                     /locus_tag="ACHKXP_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO7649099.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRADVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4815..5531
                     /gene="sdhB"
                     /locus_tag="ACHKXP_004213"
     CDS             4815..5531
                     /gene="sdhB"
                     /locus_tag="ACHKXP_004213"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO7649100.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5832..8633
                     /gene="sucA"
                     /locus_tag="ACHKXP_004214"
     CDS             5832..8633
                     /gene="sucA"
                     /locus_tag="ACHKXP_004214"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO7649101.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8648..9865
                     /gene="odhB"
                     /locus_tag="ACHKXP_004215"
     CDS             8648..9865
                     /gene="odhB"
                     /locus_tag="ACHKXP_004215"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO7649102.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            10231..11397
                     /gene="sucC"
                     /locus_tag="ACHKXP_004216"
     CDS             10231..11397
                     /gene="sucC"
                     /locus_tag="ACHKXP_004216"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO7649103.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11397..12266
                     /gene="sucD"
                     /locus_tag="ACHKXP_004217"
     CDS             11397..12266
                     /gene="sucD"
                     /locus_tag="ACHKXP_004217"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO7649104.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12370..13092)
                     /gene="mngR"
                     /locus_tag="ACHKXP_004218"
     CDS             complement(12370..13092)
                     /gene="mngR"
                     /locus_tag="ACHKXP_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO7649105.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIERSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3165 a         3332 c         3552 g         3076 t
ORIGIN      
        1 tttaataata ttaaaaagct tgtgatataa agaatggaat aattgagtgc ataaatttat
       61 tgtttaatga gatgaaggat ggggtgggat ggatatgcat aattttcatt gggttaacaa
      121 tgttaccggc agggaagtat aaaaataaac ttatcattct gctcagtatg tgacttgtct
      181 cattttttca ccttattaat gggtatttgc atctggttta atttttacaa ttttttatgt
      241 tcagccatat aaaaaagaac ccgctctatt aaaggcgggt ccggaaagta aacggcttag
      301 caatcaatca ttaacgcttg atatcgcttt taaagtcgcg tttttcatat cctgtataca
      361 gctgacgcgg acgggcaatc ttcataccgt cactgtgcat ctcgctccag tgggcgatcc
      421 agccaacggt acgtgccatt gcgaaaatga cggtgaacat ggaagacgga atacccatcg
      481 ctttcaggat gataccagag tagaaatcga cgttcgggta cagtttcttc tcgataaagt
      541 acgggtcgtt cagcgcgatg ttttccagct ccatagccac ttccagcagg tcatccttcg
      601 tgcccagctc tttcagcact tcatggcagg tttcacgcat tacggtggcg cgcgggtcgt
      661 aatttttgta cacgcggtga ccgaagccca tcaggcggaa agaatcattt ttatctttcg
      721 cacgacgaac aaattccgga atgtgtttaa cggagctgat ttcttccagc attttcagcg
      781 ctgcttcgtt agcaccaccg tgcgcaggtc cccacagtga agcaatacct gctgcgatac
      841 aggcaaacgg gttcgcaccc gaagagccag cggtacgcac ggtggaggtg gaggcgttct
      901 gttcatggtc agcgtgcagg atcaaaatac ggtccatagc acgttccaga atcggattaa
      961 cttcatacgg ttcgcacggc gtagagaaca tcatattcag gaagttaccg gcgtaggaga
     1021 gatcgttgcg cgggtaaaca aatggctgac caatggaata cttgtaacac atcgctgcca
     1081 tggtcggcat tttcgacagc aggcggaacg cggcaatttc acgatgacga ggattgttaa
     1141 catccagcga gtcgtgatag aacgccgcca gcgcgccggt aataccacac atgactgcca
     1201 ttggatgtga gtcgcgacgg aaagcgtgga acagacgggt aatctgctcg tggatcatgg
     1261 tatgacgggt caccgtagtt ttaaattcgt catactgttc ctgagtcggt ttttcaccat
     1321 tcagcaggat gtaacaaact tccaggtagt tagaatcggt cgccagctga tcgatcggga
     1381 aaccgcggtg cagcaaaata ccttcatcac catcaataaa agtaatttta gattcgcagg
     1441 atgcggttga agtgaagcca gggtcaaagg tgaacacacc ttttgaaccg agagtacgga
     1501 tatcaataac atcttgaccc agcgtgcctt tcagcacatc cagttcaaca gctgtgtccc
     1561 cgttgagggt gagttttgct tttgtatcag ccatttaagg tctccttagc gccttattgc
     1621 gtaagactgc cggaacttaa atttgccttc gcacatcaac ctggctttac ccgtttgtta
     1681 tttggctcgc cgctctgtga aagaggggaa aacctgggta cagagctctg ggcgcttgca
     1741 ggtaaaggat ccattgatga cgaataaatg gcgaatcaag tacttagcaa tccgaattat
     1801 taaacttgtc taccactaat aactgtcccg aatgaattgg tcaatacttc cacactgtta
     1861 cataagttaa tcttaggtga aataccgact tcataacttt tacgcattat atgcttttcc
     1921 tggtaatgtt tgtaacaact ttgttgaatg attgtcaaat tagatgatta aaaattaaat
     1981 aaatgttgtt atcgtgacct agatcactgt tcaggataaa acccgacaaa ctatatgtag
     2041 gttaattgta atgattttgt gaacggccta tactgccgcc aggtctccgg aacaccctgc
     2101 aatcccgagc cacccagcgt tgtaacgtgt cgttttcgca tctggaagca gtgttttgca
     2161 tgacgcgcag ttatagaaag gacgctgtct gacccgcaag cagaccggag gaaggaaatc
     2221 ccgacgtctc caggtaacag aaagttaacc tctgtgcccg tagtccccag ggaataataa
     2281 gaacagcatg tgggcgttat tcatgataag aaatgtgaaa aaacaaagac ctgttaatct
     2341 ggacctacag accatccggt tccccatcac ggcgatagcg tccattctcc atcgcgtttc
     2401 cggtgtgatc acctttgttg cagtgggcat cctgctgtgg cttctgggta ccagcctctc
     2461 ttcccctgaa ggtttcgagc aagcttccgc gattatgggc agcttcttcg tcaaatttat
     2521 catgtggggc atccttaccg ctctggcgta tcacgtcgtc gtaggtattc gccacatgat
     2581 gatggatttt ggctatctgg aagaaacatt cgaagcgggt aaacgctccg ccaaaatctc
     2641 ctttgttatt actgtcgtgc tttcacttct cgcaggagtc ctcgtatggt aagcaacgcc
     2701 tccgcattag gacgcaatgg cgtacatgat ttcatcctcg ttcgcgctac cgctatcgtc
     2761 ctgacgctct acatcattta tatggtcggt tttttcgcca ccagtggcga actgacatat
     2821 gaagtctgga tcggtttctt cgcctctgcg ttcaccaaag tgttcaccct gctggcgctg
     2881 ttttctatct tgatccatgc ctggatcggc atgtggcagg tgttgaccga ctacgttaaa
     2941 ccgctggctt tgcgcctgat gctgcaactg gtgattgtcg ttgcactggt ggtttacgtg
     3001 atttatggat tcgttgtggt gtggggtgtg tgatgaaatt gccagtcaga gaatttgatg
     3061 cagttgtgat tggtgccggc ggcgcaggta tgcgcgcggc gctgcaaatt tcccagagcg
     3121 gccagacctg tgcgctgctc tctaaagtct tcccgacccg ttcccatacc gtttctgcgc
     3181 aaggcggcat taccgttgcg ctgggtaata cccatgaaga taactgggaa tggcatatgt
     3241 acgacaccgt aaaagggtcg gactatatcg gtgaccagga cgcgattgaa tatatgtgta
     3301 aaaccgggcc ggaagcgatt ctggaactcg aacacatggg cctgccgttc tcgcgtcttg
     3361 atgatggtcg tatctatcaa cgtccgtttg gcggtcagtc gaaaaacttc ggcggcgagc
     3421 aggcggcacg cactgcggca gcggctgacc gtaccggtca cgcactgttg cacacgcttt
     3481 atcagcagaa cctgaaaaac cacaccacca ttttctccga gtggtatgcg ctggatctgg
     3541 tgaaaaacca ggatggcgcg gtggtgggtt gtaccgcact gtgcatcgaa accggtgaag
     3601 tggtttattt caaagcccgc gctaccgtgc tggcgactgg cggggcaggg cgtatttatc
     3661 agtccaccac taacgcccac attaacactg gcgacggtgt cggcatggct atccgcgcag
     3721 acgtaccggt gcaggatatg gaaatgtggc aattccaccc gaccggcatt gccggtgcgg
     3781 gcgtactggt caccgaaggt tgccgtggtg aaggcggtta tctgctgaac aaacatggcg
     3841 aacgctttat ggagcgttat gcgccgaacg ccaaagacct ggcgggccgt gatgtggtgg
     3901 cgcgttccat catgattgaa atccgtgaag gtcgcggctg tgatggtccg tgggggccac
     3961 acgcgaaact gaaactcgat cacctgggta aagaagttct cgaatcccgt ctgccgggta
     4021 tcctggagct ttcccgtacc ttcgctcacg ttgatccggt gaaagagccg attccggtta
     4081 tcccaacctg tcactacatg atgggcggta ttccgaccaa agtgaccggt caggcgctga
     4141 ctgtgaatga gaaaggcgaa gatgtggttg ttccggggct gttcgcggta ggtgaaattg
     4201 cctgcgtatc ggtacacggt gctaaccgtc tgggcggtaa ctcgctgctg gacctggtgg
     4261 tctttggtcg cgcagcgggt ctgcatctgc aagagtctat cgccgagcag ggcgcattgc
     4321 gcgatgccag cgagtctgat gttgaagcat ctctggatcg tctgaaccgc tggaacaata
     4381 atcgtaacgg tgaagatccg gtggcgattc gtaaagcgct gcaagaatgt atgcagcata
     4441 acttctcggt cttccgtgaa ggtgatgcga tggcgaaagg gcttgagcag ttgaaagtta
     4501 tccgcgagcg tctgaaaaat gcccgtctgg atgacacttc cagcgagttc aacacccagc
     4561 gcgttgagtg tctggaactg gataacctga tggaaacggc gtatgcaacg gctgtttctg
     4621 ccaacttccg taccgaaagc cgtggcgcgc atagccgctt cgacttcccg gatcgtgatg
     4681 atgaaaactg gctgtgccac tccctgtatc tgccagagtc ggaatccatg acgcgccgaa
     4741 gcgtcaacat ggaaccgaaa ctgcgcccgg cattcccgcc gaagattcgt acttactaat
     4801 gcggagacag gaaaatgaga ctcgagtttt caatttatcg ctataacccg gatgttgatg
     4861 atgctccgcg tatgcaggat tacaccctgg aagcggaaga aggtcgcgac atgatgttgc
     4921 tggatgcgct tatccagctg aaagagaaag atcccagcct gtcgttccgc cgctcctgcc
     4981 gtgaaggtgt gtgcggttcc gacggtctga acatgaacgg caagaatggt ctggcctgta
     5041 ttaccccgat ttcggcactc aaccagccgg gcaagaagat tgtgattcgc ccgctgccag
     5101 gtttaccggt gatccgcgat ttggtggtag acatgggaca attctatgcg caatatgaga
     5161 aaattaagcc ttacctgttg aataatggac aaaatccgcc agctcgcgag catttacaga
     5221 tgccagagca gcgcgaaaaa ctcgacgggc tgtatgaatg tattctctgc gcatgttgtt
     5281 caacctcttg tccgtctttc tggtggaatc ccgataagtt tatcggcccg gcaggcttgt
     5341 tagcggcata tcgtttcctg atcgatagcc gtgataccga gactgacagc cgcctcgacg
     5401 gtttgagcga tgcattcagt gtattccgct gtcacagcat catgaactgc gtcagtgtat
     5461 gtccgaaggg gctgaacccg acgcgcgcca tcggccatat caagtcgatg ttgttgcagc
     5521 gtaatgcgta aaccgtaggc ctgataagac gcgcaagcgt cgcatcaggc aaccagtgcc
     5581 ggatgcggcg tgaacgcctt atccggccta caagccatta cccgtaggcc tgataagcgc
     5641 agcgcatcag gcgtaacaaa gaaatgcagg aaatctttaa aaactgcccc tgacactaag
     5701 acagttttta aaggttcctt cgcgagccac tacgtagaca agagctcgca agtgaacccc
     5761 ggcacgcaca tcactgtgcg tggtagtatc cacggcgaag taagcataaa aaagatgctt
     5821 aagggatcac gatgcagaac agcgctttga aagcctggtt ggactcttct tacctctctg
     5881 gcgcaaacca gagctggata gaacagctct atgaagactt cttaaccgat cctgactcgg
     5941 ttgacgctaa ctggcgttcg acgttccagc agttacctgg tacgggagtc aaaccggatc
     6001 aattccactc tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct tcacgttact
     6061 cttcaacgat ctccgaccct gacaccaatg tgaagcaggt aaaagtcctg cagctcatta
     6121 acgcataccg cttccgtggt caccagcatg cgaatctcga tccgctggga ctgtggcagc
     6181 aagataaagt ggccgatctg gatccgtctt tccacgatct gaccgaagca gacttccagg
     6241 agaccttcaa cgtcggttca tttgccagcg gcaaagaaac catgaaactc ggcgagctgc
     6301 tggaagccct caagcaaacc tactgcggcc cgattggtgc cgagtatatg cacatcacca
     6361 gcaccgaaga aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg actttcaata
     6421 gcgaagagaa aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt gaacgttacc
     6481 tcggcgcaaa attccctggc gcaaaacgct tctcgctgga aggcggtgac gcgttaatcc
     6541 cgatgcttaa agagatgatc cgccacgctg gcaacagcgg cacccgcgaa gtggttctcg
     6601 ggatggcgca ccgtggtcgt ctgaacgtgc tggtgaacgt gctgggtaaa aaaccgcaag
     6661 acttgttcga cgagttcgcc ggtaaacata aagaacacct cggcacgggt gacgtgaaat
     6721 accacatggg cttctcgtct gacttccaga ccgatggcgg cctggtgcac ctggcgctgg
     6781 cgtttaaccc gtctcacctt gagattgtaa gcccggtagt tatcggttct gttcgtgccc
     6841 gtctggacag acttgatgag ccgagcagca acaaagtgct gccaatcacc atccacggtg
     6901 acgccgcagt gaccgggcag ggtgtggttc aggaaactct gaacatgtcg aaagcgcgtg
     6961 gttatgaagt tggcggtacg gtacgtatcg ttatcaacaa ccaggttggt ttcaccacct
     7021 ctaatccgct ggatgctcgt tctacaccgt actgtactga tatcggtaag atggttcagg
     7081 caccgatttt ccacgttaac gcggatgatc cggaagccgt tgcctttgtg acccgtctgg
     7141 cgctcgattt ccgtaacacc tttaaacgtg atgtcttcat cgacctggtg tgctaccgcc
     7201 gtcacggcca caacgaagcc gacgagccga gcgcaaccca gccgctgatg tatcagaaaa
     7261 tcaaaaaaca tccgacgccg cgcaaaatct atgctgacaa gctggagcag gaaaaagtgg
     7321 cgacgctgga agatgccacc gagatggtta acctgtaccg cgatgcgctg gatgctggcg
     7381 attgcgtagt ggcagagtgg cgtccgatga acatgcactc tttcacctgg tcgccgtatc
     7441 tcaaccacga atgggacgaa gagtacccga acaaagttga gatgaagcgc ctgcaggaac
     7501 tggctaaacg catcagcacg gtgccggaag cggttgaaat gcagtctcgc gttgccaaaa
     7561 tttatggcga tcgccaggcg atggctgccg gtgagaaact gttcgactgg ggcggcgcgg
     7621 aaaacctcgc ttacgccacg ctggttgacg aaggcattcc ggttcgcctg tcgggtgaag
     7681 actccggtcg cggtaccttc ttccaccgcc acgcggtgat ccacaaccag tctaacggtt
     7741 ccacttacac gccgctgcaa cacatccata acggccaggg cgcgttccgt gtctgggact
     7801 ccgtactgtc tgaagaagca gtgctggcgt ttgaatacgg ttatgccacc gcagaaccac
     7861 gcactctgac tatctgggaa gcgcagttcg gtgacttcgc caacggtgcg caggtggtta
     7921 tcgaccagtt catctcctct ggcgaacaga aatggggccg gatgtgtggt ctggtgatgt
     7981 tgctgccgca cggttatgaa gggcaggggc cggagcactc ctccgcgcgt ctggaacgtt
     8041 atctgcaact ttgtgctgag caaaacatgc aggtgtgcgt accgtctacc ccggcacagg
     8101 tttaccacat gctgcgtcgt caggcgctgc gcgggatgcg tcgtccgctg gtcgtgatgt
     8161 cgccgaaatc cctgctgcgt catccgctgg cggtttccag cctcgaagaa ctggcgaacg
     8221 gcaccttcct gccagccatc ggtgaaatcg acgagcttga tccgaagggt gtgaagcgcg
     8281 tagtgatgtg ttctggtaag gtttattacg acctgctgga acaacgtcgt aagaacaatc
     8341 aacacgatgt cgccattgtg cgtatcgagc aactctaccc gttcccgcat aaagcgatgc
     8401 aggaagtgtt gcagcagttt gctcacgtca aggattttgt ctggtgccag gaagagccgc
     8461 tcaaccaggg cgcatggtac tgcagccagc atcatttccg tgaagtgatt ccgtttgggg
     8521 cttctctgcg ttatgcaggc cgcccggcct ccgcctctcc ggcggtaggg tatatgtccg
     8581 ttcaccagaa acagcaacaa gatctggtta atgacgcgct gaacgtcgaa taaataaagg
     8641 atacacaatg agtagcgtag atattctggt ccctgacctg cctgaatccg tagccgatgc
     8701 caccgtcgca acctggcata aaaaacccgg cgacgcagtc gtacgtgatg aagtgctggt
     8761 agaaatcgaa actgacaaag tggtactgga agtaccggca tcagcagacg gcattctgga
     8821 tgcggttctg gaagatgaag gtacaacggt aacgtctcgt cagatccttg gtcgcctgcg
     8881 tgaaggcaac agcgccggta aagaaaccag cgccaaatct gaagagaaag cgtccactcc
     8941 ggcgcaacgc cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa gcccggcgat
     9001 ccgtcgcctg ctggctgaac acaatctcga cgccagcgcc attaaaggca ccggtgtggg
     9061 tggtcgtctg actcgtgaag atgtggaaaa acatctggcg aaagccccgg cgaaagagtc
     9121 tgctccggca gcggctgctc cggcggcgca accggctctg gctgcacgta gtgaaaaacg
     9181 tgtcccgatg actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag cgaaaaactc
     9241 caccgccatg ctgaccacgt tcaacgaagt caacatgaag ccgattatgg atctgcgtaa
     9301 gcagtacggt gaagcgtttg aaaaacgcca cggcatccgt ctgggcttta tgtccttcta
     9361 cgtgaaagcg gtggttgaag ccctgaaacg ttacccggaa gtgaacgctt ctatcgacgg
     9421 cgatgacgtg gtttaccaca actatttcga cgtcagcatg gcggtttcta cgccgcgcgg
     9481 cctggtgacg ccggttctgc gtgatgtcga taccctcggc atggcagaca tcgagaagaa
     9541 aatcaaagag ctggcagtca aaggccgtga cggcaagctg accgttgaag atctgaccgg
     9601 tggtaacttc accatcacca acggtggtgt gttcggttcc ctgatgtcta cgccgatcat
     9661 caacccgccg cagagcgcaa ttctgggtat gcacgctatc aaagatcgtc cgatggcggt
     9721 gaatggtcag gttgagatcc tgccgatgat gtacctggcg ctgtcctacg atcaccgtct
     9781 gatcgatggt cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc tggaagatcc
     9841 gacgcgtctg ctgctggacg tgtagtagtt taagtttcac ctgcactgta gaccggataa
     9901 ggcattatcg ccttctccgg caattgaagc ctgatgcgac gctgacgcgt cttatcaggc
     9961 ctacgggacc accaatgtag gtcggataag gcgctagcgc cgcatccgac aagcgatgcc
    10021 tgatgtgacg gttaacgtgt tttatcaggc ctacggggac caccaatgta ggtcggataa
    10081 ggcgcaagcg ccgcatccga caagcgatgc ctgatgtgac gtttaacgtg tcttatcagg
    10141 cctacgggtg accgacaatg cccggaagcg atacgaaata ttcggtctac ggtttaaaag
    10201 ataacgatta ctgaaggatg gacagaacac atgaacttac atgaatatca ggcaaaacaa
    10261 ctttttgccc gctatggctt accagcaccg gtgggttatg cctgtactac tccgcgcgaa
    10321 gcagaagaag ccgcttcaaa aatcggtgcc ggcccgtggg tagtgaaatg tcaggttcac
    10381 gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa acagcaaaga agacatccgt
    10441 gcttttgcag aaaactggct cggcaagcgt ctggtaacgt atcaaacaga tgccaatggc
    10501 caaccggtta accagattct ggttgaagca gcgaccgata tcgctaaaga gctgtatctc
    10561 ggtgccgttg tagaccgtag ttcccgtcgt gtggtcttta tggcctccac cgaaggcggc
    10621 gtggaaatcg aaaaagtggc ggaagaaact ccgcacctga tccacaaagt tgcgcttgat
    10681 ccgctgactg gcccgatgcc gtatcaggga cgcgagctgg cgttcaaact gggtctggaa
    10741 ggtaaactgg ttcagcagtt caccaaaatc ttcatgggcc tggcgaccat tttcctggag
    10801 cgcgacctgg cgttgatcga aatcaacccg ctggtcatca ccaaacaggg cgatctgatt
    10861 tgcctcgacg gcaaactggg cgctgacggc aacgcactgt tccgccagcc tgatctgcgc
    10921 gaaatgcgtg accagtcgca ggaagatccg cgtgaagcac aggctgcaca gtgggaactg
    10981 aactacgttg cgctggacgg taacatcggt tgtatggtta acggcgcagg tctggcgatg
    11041 ggtacgatgg acatcgttaa actgcacggc ggcgaaccgg ctaacttcct tgacgttggc
    11101 ggcggcgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa tcatcctctc tgacgacaaa
    11161 gtgaaagccg ttctggttaa catcttcggc ggtatcgttc gttgcgacct gatcgctgac
    11221 ggtatcatcg gcgcggtagc agaagtgggt gttaacgtac cggtcgtggt acgtctggaa
    11281 ggtaacaacg ccgaactcgg cgcgaagaaa ctggctgaca gcggcctgaa tattattgca
    11341 gcaaaaggtc tgacggatgc agctcagcag gttgttgccg cagtggaggg gaaataatgt
    11401 ccattttaat cgataaaaac accaaggtta tctgccaggg ctttaccggt agccagggga
    11461 ctttccactc agaacaggcc attgcatacg gcactaaaat ggttggcggc gtaaccccag
    11521 gtaaaggcgg caccacccac ctcggcctgc cggtgttcaa caccgtgcgt gaagccgttg
    11581 ctgccactgg cgctaccgct tctgttatct acgtaccagc accgttctgc aaagactcca
    11641 ttctggaagc catcgacgca ggcatcaaac tgattatcac catcactgaa ggcatcccga
    11701 cgctggatat gctgaccgtg aaagtgaagc tggatgaagc aggcgttcgt atgatcggcc
    11761 cgaactgccc aggcgttatc actccgggtg aatgcaaaat cggtatccag ccgggtcaca
    11821 ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg tacactgacc tatgaagcgg
    11881 ttaaacagac cacggattac ggtttcggtc agtcgacctg tgtcggtatc ggcggtgacc
    11941 cgatccctgg ctctaacttt atcgacattc tcgaaatgtt cgaaaaagat ccgcagaccg
    12001 aagcgatcgt gatgattggt gagatcggcg gtagcgctga agaagaagcg gcagcgtaca
    12061 tcaaagagca cgttaccaag ccagttgtgg gctacattgc tggtgtgact gcgccgaaag
    12121 gcaaacgtat gggccacgcg ggtgccatca ttgccggtgg gaaagggact gcggatgaga
    12181 aattcgctgc tctggaagcc gcaggcgtga aaaccgttcg cagcctggcg gatatcggtg
    12241 aagcactgaa aactgttctg aaataaatat ctgtaataag aaatagccct cgccgcttcc
    12301 ctctacagga atggcgaagg gctgtcggtt tcgacatggt tggccatcgt atgatggcct
    12361 tttttgtgct tatcgcgatg attttcgctg cgctatcagg gtaaatttat agtcatcggt
    12421 attaaaagcg ttgcggctat attcaaacac ccgaccatca actaaatatc cacgcgatac
    12481 tttttcaaga atcggctttg tctggctgat attaagcaga cggctcatct cttcggttgg
    12541 catcagagga atgatttcct gttcgctacg ctcgataacc attttcttca cttcttcaat
    12601 aaagtgatat ttcgaatttt ccatgacctg ccaggtgaga tccgggaaca acgcaagcgg
    12661 catccaggtt tcttccagcg ccattggctt ttgcttgcga tagcgcacgc gcttcacatg
    12721 ccacacacga tcctgcgcgg tgatttgtag ctgttgctga agaaaatcgt cagccggaat
    12781 cacttcaaat atcaggactt cactgtgtgt atcgacgtga cggtccgaca gtttttcatc
    12841 aaaactggtt aactgaaaaa tatcgtaatt gacccgctct tctttgacgt aagtcccgct
    12901 gccctgaatg ctttcgagga tctgctgctc gactagctgg cgcaaagcct gacgcaccgt
    12961 aacccggctg acgccaaact ctgtttgtaa tgcagattcg gtaggtaacg catcgccagg
    13021 tttaagctcc ccacgcgcaa tttgctcacg aatgcgatcg gcaatctgcc ggtataaggg
    13081 tttgtgtccc atttttagta tctcattaat acgaatttag ccatt
//
LOCUS       DAZFZC010000120        12975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFZC010000120 DAZFZC010000000
VERSION     DAZFZC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.121"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            335..916
                     /gene="mdaB"
                     /locus_tag="ACHKXP_004219"
     CDS             335..916
                     /gene="mdaB"
                     /locus_tag="ACHKXP_004219"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO7649106.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            947..1261
                     /locus_tag="ACHKXP_004220"
     CDS             947..1261
                     /locus_tag="ACHKXP_004220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO7649107.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1309..3201)
                     /gene="parE"
                     /locus_tag="ACHKXP_004221"
     CDS             complement(1309..3201)
                     /gene="parE"
                     /locus_tag="ACHKXP_004221"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO7649108.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3230..3811)
                     /gene="yqiA"
                     /locus_tag="ACHKXP_004222"
     CDS             complement(3230..3811)
                     /gene="yqiA"
                     /locus_tag="ACHKXP_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO7649109.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3811..4638)
                     /gene="cpdA"
                     /locus_tag="ACHKXP_004223"
     CDS             complement(3811..4638)
                     /gene="cpdA"
                     /locus_tag="ACHKXP_004223"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO7649110.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPGRHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4663..5085)
                     /locus_tag="ACHKXP_004224"
     CDS             complement(4663..5085)
                     /locus_tag="ACHKXP_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO7649111.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5086..5715)
                     /gene="nudF"
                     /locus_tag="ACHKXP_004225"
     CDS             complement(5086..5715)
                     /gene="nudF"
                     /locus_tag="ACHKXP_004225"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO7649112.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5920..7401
                     /gene="tolC"
                     /locus_tag="ACHKXP_004226"
     CDS             5920..7401
                     /gene="tolC"
                     /locus_tag="ACHKXP_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO7649113.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7549..8220
                     /locus_tag="ACHKXP_004227"
     CDS             7549..8220
                     /locus_tag="ACHKXP_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO7649114.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8226..9386
                     /locus_tag="ACHKXP_004228"
     CDS             8226..9386
                     /locus_tag="ACHKXP_004228"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7649115.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9424..10212)
                     /gene="ygiD"
                     /locus_tag="ACHKXP_004229"
     CDS             complement(9424..10212)
                     /gene="ygiD"
                     /locus_tag="ACHKXP_004229"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO7649116.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQIG"
     gene            10355..11128
                     /gene="zupT"
                     /locus_tag="ACHKXP_004230"
     CDS             10355..11128
                     /gene="zupT"
                     /locus_tag="ACHKXP_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO7649117.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11186..11356)
                     /gene="yqiD"
                     /locus_tag="ACHKXP_004231"
     CDS             complement(11186..11356)
                     /gene="yqiD"
                     /locus_tag="ACHKXP_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO7649118.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11618..12271)
                     /gene="ribB"
                     /locus_tag="ACHKXP_004232"
     CDS             complement(11618..12271)
                     /gene="ribB"
                     /locus_tag="ACHKXP_004232"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO7649119.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12375..12523)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12645..12935
                     /gene="ubiK"
                     /locus_tag="ACHKXP_004233"
     CDS             12645..12935
                     /gene="ubiK"
                     /locus_tag="ACHKXP_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO7649120.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3280 a         3465 c         3165 g         3065 t
ORIGIN      
        1 ttttctgctt taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga
       61 cacgttgata ctttcatctt ttacttattc aaagccatat tttcaatccc caaaaaaacc
      121 gaaccaaacg tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat
      181 tgtcattttc agaaaatatt tatcctgcat cggtaagtca gagtaagatc agacttttgc
      241 taaattcgca aaagactttg cacattttgc taatttcacc gtaccgctct gtgacgtact
      301 atagtcggca aacgtctcac cttgaggtta aaaaatgagc aacatcctga ttatcaacgg
      361 cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga
      421 tggcacactg cgcgaccttg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacga
      481 tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg
      541 ctggtggatg ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg
      601 tcatggaacg ctgtatgcca gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg
      661 ttccggcggc ctggtacagg gcaaaaaata tatgctttct ctgacctgga acgcaccaat
      721 ggaagccttc accgaaaaag atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct
      781 gccgttccat aaagcaaacc aattccttgg catggaaccg ctgccgacat ttatcgctaa
      841 tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt
      901 ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat
      961 cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc
     1021 taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga
     1081 ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca
     1141 gtgggaaagc atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag
     1201 cgaagccgta aaaggcgacg tgctggagat gaatatccgc atcctgcaac ctgggattta
     1261 aacctgcctt gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat
     1321 ctccgccatg tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc
     1381 cagcagcata tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg
     1441 caccagacgg cgagtgttcg gatcaagcgt ggtttcgcgc aattgcatcg ggttcatttc
     1501 cccgagacct ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag
     1561 tacgccttct ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg
     1621 gtagagcggt ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt
     1681 tacgaacaaa gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag
     1741 gatacagatt ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat
     1801 cgctaccgaa atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca
     1861 ggtgttaagg atcttacctt tcagtggcat gatcgcctga tattcgcgat cgcgcgcctg
     1921 cttggcagat ccgcctgcag agtcaccttc cacaaggaac agttcggtac ggttaagatc
     1981 ctgcgcggta cagtcagcca gtttgccagg cagcgccggg ccgctggtca gctttttacg
     2041 caccactttc ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag
     2101 caactccgcc gcctgaacgt tctggttcag ccacaggata aaggcatctt tcaccacgcc
     2161 agaaacgaat gccgcgcact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg
     2221 cggatcctgc atttttactg acagcacata ggcgcagcga tcccagatat cttccgccga
     2281 caactttaca ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa
     2341 caggccctga cgcagaccgt taacgtgcgt accgccctgc atcgttggga taaggttgac
     2401 gtagctttcg gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc
     2461 ttcagtatca ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt
     2521 taccgcttcc gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt
     2581 gttgatctca tctttaaagg taatctcaac gccggggcac agtaccgctt tggctttcag
     2641 cacatgcgtc aggcgtgaaa cagaaaatcg cgggctgtca aagaaggttt catccggcca
     2701 gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg
     2761 caccttttcg ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac
     2821 ttcaacgcgc ttcgacaggg cgttaaccac cgaaatcccc acgccatgca ggccgccaga
     2881 gaactggtaa tttttgttag agaatttacc gcccgcgtgc aaacggcaaa gaatcagttc
     2941 aaccgccggt acaccctctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat
     3001 cacttctaac gactggtcag catgtaagat tacgtccacg cgttttgcgt gacccgccag
     3061 tgcttcatcg acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc
     3121 ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc
     3181 agcgttataa gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt
     3241 gcagaccaag aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt
     3301 tgccgccttc tatgacagtc tggcggcagg aagcgtagta cgccaccgcc tggcggtaat
     3361 ccagcacttc atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt
     3421 caatctgcat gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg
     3481 tgtaggggtt ctcgttctga ccgagatagt ccgtcagcag ttcaaacggg cgtaccgccg
     3541 ggtttaccac cactgcgggc agcataaaac attgcgacaa ccaggtggcg taatatcccc
     3601 ccagtgacga accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca
     3661 gcagttctgc cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag
     3721 ggtgatgttc cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt
     3781 tgaaaccgtg taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg
     3841 gaaacgtgtg tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc
     3901 gagagtacgc cagccgggcg cgatggtgtc cagcgtaaag ttggaacagt gcggcttaaa
     3961 ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc
     4021 ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc
     4081 cagttcgccc gcgttacgca ggctgtgttg atcgagccaa ctacaacccg caggtagcgg
     4141 atgatgatgc agcagcagca acgtatggcg tcctggcgca tcggccagtt tacgttccag
     4201 ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag
     4261 caacaggatt tgccattgct caccaataaa cacgcgcttc gccggggaga tacccgcatc
     4321 ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg
     4381 cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg
     4441 cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc
     4501 cagcaccgcc tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc
     4561 aaacaggtga gtatcggtaa tttgtaaaat cctgactctg gcctcaccag ccagaggaag
     4621 ggttaacagg ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc
     4681 catcgctcca tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg
     4741 cttttcgtcg cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa
     4801 aatctgctgg cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat
     4861 tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt
     4921 gtatcgggtc gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac
     4981 agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc
     5041 gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt
     5101 ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc
     5161 gattttcccc tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat
     5221 atcttcgttt tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc
     5281 caccataatt gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg
     5341 tttggtccgt ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc
     5401 cacactttca ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcact
     5461 ggtgtcgtat gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc
     5521 aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgca cctcatgact
     5581 catttgcccg ttgaatagac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg
     5641 ataaagtgtt tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt
     5701 gtctggctta agcattgtta atgtcctggc actaatagtg aattaaatgt gaatttcagc
     5761 gacgtttgac tgccgtttga gcagtcatgt gttaaattga ggcacattaa cgccctatgg
     5821 cacgtaacgc caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt
     5881 ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt
     5941 cttatcggcc tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa
     6001 gtttatcagc aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat
     6061 gctgcctttg aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt
     6121 gcagattaca cctatagcaa cggctaccgc gacgcgaacg gcatcaactc taacgcgacc
     6181 agtgcgtccc tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg
     6241 ctgcaggaaa aagcagcagg gattcaggac gtcacgtatc agaccgatca gcaaaccttg
     6301 atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat
     6361 acacaggcac aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac
     6421 gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg cacagtacga taccgtgctg
     6481 gcgaacgaag tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc
     6541 accggtaact actatccgga actggctgcg ctgaatgtcg aaaactttaa aaccgacaaa
     6601 ccacagccgg ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag
     6661 gcacgcttga gccaggacct ggcgcgcgag caaattcgcc aggcgcagga tggtcactta
     6721 ccgactctgg atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa
     6781 acccgtggtg ccgctggtac ccagtatgac gatagcaata tgggccagaa caaagttggc
     6841 ctgagcttct cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca
     6901 cagtacaact ttgtcggtgc cagcgagcaa ctggaaagtg cccatcgtag cgtcgtgcag
     6961 accgtgcgtt cctccttcaa caacattaat gcatctatca gtagcattaa cgcctacaaa
     7021 caagccgtag tttccgctca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt
     7081 acgcgtacca ttgttgatgt gttggatgcg accaccacgt tgtacaacgc caagcaagag
     7141 ctggcgaatg cgcgttataa ctacctgatt aatcagctga atattaagtc agctctgggt
     7201 acgttgaacg agcaggatct gctggcactg aacaatgcgc tgagcaaacc ggtttccact
     7261 aatccggaaa acgttgcacc gcaaacgccg gaacagaatg ctattgctga tggttatgcg
     7321 cctgatagcc cggcaccagt cgttcagcaa acatccgcac gcactaccac cagtaacggt
     7381 cataaccctt tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc
     7441 aacgtaaaga tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag
     7501 ccatcctcta ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca
     7561 aaatccatac gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc
     7621 gctctcgcgg ttgccactgt ttttatgctg gctggctgtg aaaagagtga tgaaacagtg
     7681 tctctctatc aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt
     7741 accaccgcgt acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc
     7801 cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc agcaggcacc agcccaggct
     7861 ggcatggcac cagaaaacca ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg
     7921 ctgatggccg gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag
     7981 ccgctgttct cctcgaaaaa cccagccagt ccggcttacg gtaaatatac cgacgcgacg
     8041 ggtaaaaact atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg
     8101 gcaccaaaac cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc
     8161 aaacaaagca ctatgcagcg tagtgcaacc ggtacctctt ctcgttcaat gggtggctga
     8221 taccgatgga aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat
     8281 acggtttcaa ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca
     8341 agttgaccct cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt
     8401 gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc
     8461 caaaacacac ctggagtttt gtgcgccagt catggctgac gcaccagcca tcgctttatt
     8521 cgcgtcttga tctggcgtgg gatggcactg gtgaacctaa acttctggaa aataacgccg
     8581 atacgccaac gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc
     8641 ttaacgccgg taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga
     8701 tcgatcgctt cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct
     8761 gtcgcgacac ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaacggaag
     8821 ctgaaattgc tactgagttc ctctacatcg atgatatcgg gttaggtgaa aaaggtcagt
     8881 tcacggattt acaggatcag gtaatttcca acctgttcaa actgtatccg tgggaattta
     8941 tgttgcgtga gatgttctca accaagctgg aggatgcagg cgtacgctgg ctggaaccgg
     9001 cgtggaagag cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga
     9061 atcacccgaa cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat
     9121 atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca
     9181 aaaccattga agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc
     9241 acccgttacc gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc
     9301 aacccgccgg aattggcatt cgtgaagacc gtgcattgat cacccaggat atgtctcggt
     9361 tttatccaca tatttttgtt gaataagcca cgataccgga tggcactcgc catccggtaa
     9421 ttgttagcct atctgcaccg acagcatact caggctgccc atttctatac cctcaaccgg
     9481 aatggtaatt ggctcctgcc catcccacgc acctaacaca tacaacaacg gcaaataatg
     9541 ctctggcgtt gggttcgata acgtgccacc ttcatggtcg aggtaattca ccagaggatg
     9601 ttgttccact ggcccttgcc acgtcagatt cgctttcaca tactcattaa acgacgtcgc
     9661 ccatggatac ggtgaactat caccgtgcca cttcactgtg cgcaggttat gcaccacgtt
     9721 accgctggcg accaacatta ttccttcatc tcgcaacgct gccagtttgc gccccatttc
     9781 gaaatgccag gcggcaggtt tgctactgtc gatactcaac tgcaccatcg ggatatcagc
     9841 gtcaggatac atcttaatca gcacgcccca cgagccgtgg tcaaagcccc aggcttcttt
     9901 atccagcgtc accgggatcg gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga
     9961 acccggagca ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat
    10021 cgtgggcggc gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac
    10081 caccacaatc gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata
    10141 caaattatct tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg
    10201 tgttgaagac atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc
    10261 ttgttcggcg gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat
    10321 ctgcctgaag gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat
    10381 actggcgggg gcagccacgt ttattggcgc gtttctcggc gttctcgggc aaaaaccctc
    10441 gaaccgctta ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc tcatctcatt
    10501 aatggaaatg cttcctgccg cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg
    10561 aatgtttatc ttcggtctgc ttggctattt tggcctggac cgcatgttgc cacatgctca
    10621 tccgcaggat ttaatgcaaa aatcggtgca gccgttgcca aaatcgatca agcgcacagc
    10681 cattctgctc actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt
    10741 cacggcgagc agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa
    10801 tatccctgaa ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac
    10861 cgcgattctg tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg
    10921 gttaatcctc ggtagcatga tttccccggt ggtcatggcg gcaatcatgg cggcggttgc
    10981 aggaattatg gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc
    11041 taataataac cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt
    11101 gctgctacaa acggcgggaa ttggttaaaa acaacatcag aagttgtcca gcgcatggac
    11161 aacttctgcc agcttcacga aagccttatt ctaccttttc ggctgtctct tctctcgtac
    11221 tgtttaagta tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac
    11281 aataacgttt caaatgcagg aaataaccca ccacaaccag agcaatcaat acaatccagt
    11341 accaggcgat aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgtata
    11401 aatttcacat atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata
    11461 tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag
    11521 aagcggaatt aacatcttgc atagcaggga aaaataaaaa ccgaagcctg ctacaacttc
    11581 ggtttcttga ttaaggcagt aaattaagca gcggttttca gctggcttta cgctcatgtg
    11641 cctgacggta tgccaccagg tcttcaatag tcacgagcgc catattgtgt ttattggcaa
    11701 actcaataca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc tcacacagta
    11761 caccagccgg tttaaagcct gccagcgtca tcagatcaat agttgcttca gtatgaccgc
    11821 cacgcgtcag tacaccacct gcctgagcgc gaagtgggaa aacgtggcca ggacgattca
    11881 gatctgacgg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata cggtcagcgg
    11941 cagaaacacc ggtagtcaca ccttcagctg cttcaatggt cacggtaaaa ccggtgccat
    12001 aggcgctggt gttattttct accatcattg gcagatcgag ttgtttacgg cgatcttcag
    12061 taatgcacag gcaaacaata ccgctaccgt ggcgaatggt cagcgccatc tgctcaacag
    12121 tcatggtttc tgccgggaag atcatatcac cttcgttttc acggtcttca tcatcaagca
    12181 ccattacacc gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg
    12241 taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa acctcactaa aattatggtt
    12301 accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc
    12361 atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac
    12421 accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg
    12481 atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct
    12541 aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact tacccttatc
    12601 gcactacaat ggcactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa
    12661 aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg
    12721 gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc gcagctgacg cgcctcgacc
    12781 ttgtaagccg tgaagagttc gacgtccaaa cgcaagtcct gttacggact cgtgaaaaac
    12841 tggcgctgct tgaacaaaga atcagcgaac tggaaaatcg cagtactgag atcaaaaagc
    12901 agccagatcc agagactctc cccccaacgc tgtaaacatt cgtctttaat gcactgtaaa
    12961 ttatgaatta atcct
//
LOCUS       DAZFZC010000121        12966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFZC010000121 DAZFZC010000000
VERSION     DAZFZC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.122"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(164..2125)
                     /gene="topB"
                     /locus_tag="ACHKXP_004234"
     CDS             complement(164..2125)
                     /gene="topB"
                     /locus_tag="ACHKXP_004234"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO7649121.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2130..3173)
                     /gene="selD"
                     /locus_tag="ACHKXP_004235"
     CDS             complement(2130..3173)
                     /gene="selD"
                     /locus_tag="ACHKXP_004235"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO7649122.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3290..3841)
                     /locus_tag="ACHKXP_004236"
     CDS             complement(3290..3841)
                     /locus_tag="ACHKXP_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO7649123.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4002..5858
                     /gene="sppA"
                     /locus_tag="ACHKXP_004237"
     CDS             4002..5858
                     /gene="sppA"
                     /locus_tag="ACHKXP_004237"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO7649124.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLFPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAEQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6025..7041
                     /gene="ansA"
                     /locus_tag="ACHKXP_004238"
     CDS             6025..7041
                     /gene="ansA"
                     /locus_tag="ACHKXP_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO7649125.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELHEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7052..7693
                     /gene="pncA"
                     /locus_tag="ACHKXP_004239"
     CDS             7052..7693
                     /gene="pncA"
                     /locus_tag="ACHKXP_004239"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO7649126.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLHQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7786..9144)
                     /locus_tag="ACHKXP_004240"
     CDS             complement(7786..9144)
                     /locus_tag="ACHKXP_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7649127.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWLLSGKYFIESPRWLAGKGQIAGAECQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFIVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9262..10020)
                     /locus_tag="ACHKXP_004241"
     CDS             complement(9262..10020)
                     /locus_tag="ACHKXP_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7649128.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10157..11137)
                     /gene="ydjG"
                     /locus_tag="ACHKXP_004242"
     CDS             complement(10157..11137)
                     /gene="ydjG"
                     /locus_tag="ACHKXP_004242"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO7649129.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALIINLSDADATLMREMAEALER"
     gene            complement(11147..12094)
                     /locus_tag="ACHKXP_004243"
     CDS             complement(11147..12094)
                     /locus_tag="ACHKXP_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7649130.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12099..12935)
                     /locus_tag="ACHKXP_004244"
     CDS             complement(12099..12935)
                     /locus_tag="ACHKXP_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO7649131.1"
                     /translation="MLADIRYWENNATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPYQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
BASE COUNT         3269 a         3405 c         3004 g         3288 t
ORIGIN      
        1 gctcccagga tggttttgta ataaaacttt tttcatcaca ttccctgtta ttacatactt
       61 tagaggcgat aattttatcc atgcaaaaaa aatatccaac gaattttctt gatccgctgg
      121 atatttcaga atatgactcg aatagcacga aagattcact cgcttacgct atcgccccgc
      181 ttccgacttc atctgctggc ggactttttt tcgcactacg tttacgcggt gcagcctttt
      241 tcttatcagc actgccacca ctgcccggag ccacaatgcc gcgaaactgc cgcaccggcg
      301 tacgtttggc ttgatcaata agctgatata gcgtccccac cagcggctgc ataaagtcct
      361 gatagcgaca ctgcttttcg ctgatttgcg tcagcaccga ttcccagtgc gcggtcatgt
      421 ccggtcgcgt cgccatctcc ggcagcgaat ggaatagcgc ttttccggcg tcggtggagt
      481 ggatatagcg cccttttttg gtcaggaaac cacgcttgaa caacagttca ataatcccgg
      541 cacgcgttgc ctctgtcccc agaccatcgg tcgcacggag gatctttttc agatctttat
      601 cctgcacaaa gcgcgcgatc ccggtcatcg ccgaaagcag tgttgcatcg gtaaaatggc
      661 gcggcggctg ggtttgccgc tctaccactt cacctttttc acacagcaac tcatcgcctt
      721 tcgccaccac aggcagtggc gtgccgtcgt tttcttcatc gcgctctttg ctgcctaaca
      781 gcgtgcgcca gcctgcttca gcaagaaaac gcgctttagc gacaaatttg cctttggcaa
      841 tgtccagttc gataacacac ttgcggaaca ccgcatccgg gcagaattgc atcagatact
      901 gacgggcaat caggttatag accttcgctt cgttctccgt caggttgatc gcagaactcc
      961 gtgcggtcgg aatgatggcg tggtgcgcat cgaccttttt gtcatcccaa cagcggttgc
     1021 gtatatctgg atctaccact ggctgcggca acagatccgg tgcatgaaca ctgatggcat
     1081 tcatcaccgc gtggcgtccg gcaaaatgtt cttctggcaa atagcgacaa tcagaacgcg
     1141 gataagtgat tagcttgtgc gtttcgtaca gtttctggca gatatcaagc acgttctgcg
     1201 cactcagacc aaaacgtttt gccgcttcaa tctgcaacgc tgaaagcgaa aaaggcagcg
     1261 gcgcggattc tgattcccgt ttatcgttat agctggtgac aatagccggt tgaccactaa
     1321 tgcggttaac cacatgctcc gccagtggac gatgtaacaa gcgcccttct tcatcctggt
     1381 acggttcaca cgcttcgctc ggttgccaga tagcggtaaa ccgctcatcg gcaggtgtca
     1441 cgatatgtgc tttgacttca aagaagtctt tcgccacgaa gttttcaatc tcttcatcgc
     1501 ggcgcaccac cagcccaagc acgggcgtct gcacgcgtcc cacggaaagt acgccctgat
     1561 aaccggcatt gcgaccgaga atggtatacg cacgggtcat attgatgccg tacagccagt
     1621 cggcacgcgc tcgcgccagc gcagaaacgc acagcggtac aaactcactg ttggaacgaa
     1681 gacggtcgat cgcccgctca accgcctgcg ggttcaggtc gtttatcaag caacgctgta
     1741 cctgctggcg cttttccggt gccagttgca gatagtccag cacttcatcc accagcaatt
     1801 gcccttcacg atccgggtcc ccggcgtgaa cgatttcgct ggcttcatgc aggaaccgtt
     1861 tgatgacgtt aagttgtttg gtcacggagg gtcggggctg taattgccac ttttccggga
     1921 caatcggcaa atccgcaaga ttccagcgcg catagcggct gtcgtaggcg tctggctgcg
     1981 cctgctcaag caggtgaccg atacaccagg tcaccacctg accattaccg cactcgataa
     2041 agccatcgcc tttccggtgc ggtttgggca ggacatcagc aatggcgcgc gccagactcg
     2101 gtttttcggc aataaacaac cgcattgaat taacgaatct caaccatggc acgaccgccg
     2161 cgcgctggca ccagttcgcc aattgccgtc agttcaatgc caaactcggc ggctgtagct
     2221 ttgacctcat tttctgcttc cggcatgacc gccagcagca aaccgccaga agtttgcgga
     2281 tcacacagca gatcgcgcac ttcacgcggc atttcaccca tcagatgacc gtagctggca
     2341 aagttacgtt cagtgccgcc aggtactgcg cccaacttaa tgtactcttc aacaccgggg
     2401 agtttcggga tcgcttcata gtcgacgcgt gcctgcacac cagccccctg acacatttcg
     2461 ctcaagtggc ccagcagacc aaagcccgta acgtcggtca tcgcttttac gccttcgatg
     2521 ttggcaaagg acgcgcctgc gatgttcatc cggcacatca cttccgtcgc cagtccctga
     2581 tgttctggtt tcaacagtga ttttttctca gccgtggtaa gaacgccgat ccccagcggt
     2641 ttcgtcagga acagtttgca tccggcttgt gcggtactgt ttttcttcac ccgctcggtc
     2701 ggtacgatcc ccgttaccgc cagaccaaaa atcggctccg gcgcatcgat ggagtgaccg
     2761 ccagccagcg caatacccgc ctgacgacat gcatagcgtc caccttcggt cacttcgcgg
     2821 gcaatttctg gggaaagttt gttaatcggc cagccgagga tcgcaatcgc cataatcggt
     2881 ttgcccccca tcgcgaagat atcgctgatg gcgttagtcg ccgcaatgcg gccaaaatcg
     2941 aaaggattat caacgatcgg cataaagaag tcggtggtac tgataacgct ggtgccattg
     3001 cccagatcgt acaccgccgc atcgtcgcgg gtttcattac ccacaagcaa attcggatca
     3061 acaaacttcg cctgctcact atgcaggatg gtttccaaca cttttgggga aattttacag
     3121 ccgcaaccag ctccgtggct gtattgggtc aaacgaatcg agttctcgct catggacatc
     3181 tcctgtcaat gcaagccggg tatggtaacc ctcattccgt gaagtgataa gtgagagtgt
     3241 ctgaattcct gcgcctttgc tcacaatcca gacagtttcg cgacaattat cagaaataag
     3301 ttacaaacgg cgtcgggtcc gggacgttaa tcgacgtaga tgctttcagc tgcggcgtac
     3361 cgaggtagag aaaaccgaca attttatcct gctcacggca accgaatgct tcacgcacta
     3421 ccggactttc agttaatgcg ccactgcgcc agatgccgcc aaacccctgg gcaactgctg
     3481 ccatttgcat cgccatgacc gcgcatccgg cagacatttc ctgttcccag cgcgggactt
     3541 tatgattctc ttcgcatttc gccaccaccg tgatgatgag cggtgcgcgg aacggcgcat
     3601 tacgggcttt gtcgatagct ttgtcatcac taccggcagc aatcgccccc tgttccagta
     3661 cggcgctgaa acgctcgcgc ccttcccctt caatcacaaa aaaatgccac ggttgcatgg
     3721 acttatggtc cggcgcacgc atacccgcac gcaggatgtt ttgcagttgt tcacccgttg
     3781 gcgcgggttc agccaagcgg gaggcgctac ggcgattgat caatagttcg agtgcatcca
     3841 tttgattaac tcctgtcgtg atatttattc acaaaattaa cacgagagtg gattttgtta
     3901 cagcacagtc cgcaattcct gctgacaagt accggttggg tcattacgat aaccacatct
     3961 attgcgcctg tgacaggtgt gaccttaagt tgggagaata catgcgaacc ctttggcgat
     4021 ttattgccgg attttttaaa tggacgtggc gtctgctgaa tttcgtccgt gaaatggtac
     4081 ttaacctgtt ctttattttc ctcgtactgg ttggtgtggg gatttggatg caggtcagtg
     4141 gtggtgattc gaaagaaacg gccagtcgtg gcgcactgct gctggacatt tctggtgtga
     4201 tcgtcgataa acccgacagt tctcagcggt ttagtaaatt aagccgccag ctgcttggtg
     4261 ccagttccga tcgtctgcag gaaaactcac tgtttgatat cgtcaacact attcgccagg
     4321 cgaaggacga ccgcaatatc accggtattg tgatggatct gaaaaacttc gcaggcggcg
     4381 accaaccgtc tatgcagtac atcggcaaag ctctgaaaga gtttcgtgac agcgggaaac
     4441 cggtttatgc cgttggcgag aactacagcc aggggcaata ttatctcgcc agtttcgcca
     4501 ataaaatttg gctgtttccg caaggcgtgg ttgatctgca cggctttgcc accaacggtc
     4561 tgtactacaa atcgttgctg gataagctga aagtttccac ccatgtgttc cgcgtgggta
     4621 cgtataaatc tgccgttgaa ccgtttattc gtgatgatat gtcaccggca gcccgcgaag
     4681 ctgacagccg ctggattggt gagctgtggc aaaactatct gaatactgtt gccgctaacc
     4741 ggcagatccc tgctgagcag gtattccctg gcgcgcaagg gttgcttgag ggtttaacca
     4801 aaaccggtgg cgataccgcg aaatatgcac tggaaaacaa gctggtcgat gcactggcat
     4861 cgagtgcgga aatcgaaaaa gcactgacca aagaattcgg ctggagtaag actgataaaa
     4921 attatcgcgc catcagttat tacgattacg cattgaaaac gccggcagat accggtgaca
     4981 gcatcggtgt cgtctttgct aatggcgcaa ttatggatgg cgaggaaact caggggaatg
     5041 ttggcggtga taccactgcg gcacaaatcc gcgacgctcg ccttgacccg aaagtgaaag
     5101 cgattgtcct gcgtgttaat agcccaggcg gcagcgttac cgcgtctgaa gtgattcgcg
     5161 ctgaactggc agcagcccgg gcagcgggta agcctgtggt tgtatcgatg ggcggcatgg
     5221 cggcatctgg tggttactgg atttccacgc cagctaatta cattgtggct aaccccagca
     5281 ccctgaccgg ttctatcggt atcttcggcg tgatcaccac cgtagaaaat agtctggatt
     5341 cgattggtgt tcataccgat ggtgtctcaa cttcaccgct ggcagatgtt tctatcacca
     5401 gggcactgcc gccggaagca cagcagatga tgcagttaag cattgagaat ggctataaac
     5461 gctttatcac gctggttgct gatgcgcgtc attcgacgcc ggagcagatt gataaaatcg
     5521 cccagggcca cgtctggacc ggtcaggatg caaaagctaa cgggctggtc gatagcctcg
     5581 gggatttcga tgatgcagtt gccaaagcag cagagctggc aaaagtgaaa cagtggcatc
     5641 tggaatacta cgttgatgaa ccgaccttct tcgacaaagt gatggacaac atgtctggtt
     5701 ctgtccgggc aatgttgcca gatgcgttcc aggccatgtt acctgcaccg ctggcctcgg
     5761 tagcctctac tgttaaaagt gaaagcgaca agctggccgc gtttaacgac ccacaaaacc
     5821 gttatgcgtt ttgcctgacc tgcgccaacg tgcgttaagt cttgtactga gtggccgaca
     5881 gatcgtcggc cacattattt tttacgtcga cgaatcctct tcccgctgtc tcgccccata
     5941 tttccttatc cgcatagtat caggtgcgct cctcctgcct cacgtatata cttttgctct
     6001 ttcgatatca ttcatatcaa tatcatgcaa aagaaatcaa tttacgtcgc ctacacgggc
     6061 gggaccatcg ggatgcagcg ttccgagcag ggttatattc cggtgtcagg tcatctacaa
     6121 cgccaactgg cgctgatgcc ggaattccat cgcccggaga tgccagattt caccattcat
     6181 gaatatacgc cgttgatgga ttcttctgat atgactccag aagactggca gcatattgct
     6241 gaagatatta aagcgcacta tgacgactat gatggttttg tcattctgca cggcaccgat
     6301 acgatggcat ataccgcctc tgcgctgtcg ttcatgctcg agaatctcgg taaaccagtc
     6361 attgtgacag ggtcacaaat cccgctagct gagttacgct ctgacggaca aattaatctg
     6421 ctgaatgcgt tgtacgttgc ggcgaattat ccgatcaacg aagtaacgct ctttttcaat
     6481 aaccgattgt atcgcggcaa ccgcactacc aaagcccatg ccgatggttt tgatgcgttt
     6541 gcctctccaa accttcctcc gttactggaa gcaggtatcc atattcgtcg tttaaatacg
     6601 ccacccgccc cgcacggtga aggggaatta atcgttcatc caatcacccc acaaccaatt
     6661 ggcgtagtga cgatttatcc agggatttct gctgacgtcg tgcgcaattt tctgcgccaa
     6721 ccggtgaaag cattgatttt gcgctcctat ggcgtgggta atgcgccaca aaacaaagcc
     6781 ttcctgcagg aattacatga agccagcgat cgcggtattg tggtggtcaa cctgacacaa
     6841 tgtatgtccg gtaaagtgaa catgggtggt tatgccaccg gtaacgccct cgcccatgcc
     6901 ggcgttattg gcggtgcaga tatgactgta gaagccacgc taaccaaact gcattacctg
     6961 ctgagccagg aactggatac tgaaaccatt cgcaaggcca tgagccaaaa cctgcgcggc
     7021 gaactgacgc cggatgatta aggagactgc aatgccccct cgcgccctgt tactggtcga
     7081 tttacaaaat gatttctgtg ctggtggcgc gctcgccgtg ccggaaggtg acagtacggt
     7141 ggatgtcgct aaccgcctga ttgactggtg ccagtcgcgc ggtgaagcgg ttatcgccag
     7201 tcaggactgg cacccggcga atcacggcag ttttgccagt cagcacggtg tagagcctta
     7261 tacgccaggc caactcgacg gtttgccaca aaccttctgg ccagatcact gtgtgcagaa
     7321 cagtgaaggc gcacaattac atccgttact gcaccaaaaa gcgatcgcag cggtgttcca
     7381 taaaggcgaa aatcctttag ttgacagtta cagtgccttt tttgataacg gccgtcggca
     7441 gaaaacctct ctcgatgact ggttacgcga tcatgaaatc gatgaattga tcgttatggg
     7501 cctggctact gactattgcg tgaagtttac cgtgctggac gcgttacagt taggttataa
     7561 ggtaaacgtg attaccgatg gttgtcgtgg cgtgaatatc cagccacagg acagtgcgca
     7621 cgcgtttatg gagatgtcag ctgctggggc aacgctatat acgctggcag actgggaaga
     7681 gacacagggg taattttacg ctggcctaca attctgtact ggcattgtag gccaaataaa
     7741 acacgtcagt ggcacatctg gcaattgatg ccatcaacga aagatttaat tcacctcaga
     7801 aatctcttcc aacgacacct tccgcgtttc gatgccaaaa atggagagaa ccagcgcaca
     7861 gagcaataac atgacaccaa gtactataaa caccgtgatc gacccataat gtgttaataa
     7921 tgccgcaacg ccatagggcg tgaagactgc gacgatccgt ccgacggcat taacgaaacc
     7981 cgaaccgcgc aggcgtaaat gcgttggcca aagctccggg atataaaccg ccgacgcgaa
     8041 gcaaacatac atgtataaaa agaagatcat caccaggcca tagattaaaa tcgcccactc
     8101 tgtagtctga attgaataga tatagcctaa cacggcaata ataataagta aggtggagcc
     8161 aaataaccga cgaggaaaat gatcaataat taatgccgca ataaatattc ctaccggagc
     8221 gccaatcata ataacagcgg tcattaatat tgatttatcg acatcaatgc cggagttaac
     8281 aaatatggtc ggtatccata cggtgatggt ataaagcgaa atgttcattg caattaaaac
     8341 agtaatcgcg actaatgtac gtcgtaacat ttcaccttta aacaggagcc agaaagtacc
     8401 cttgattact ttaactttgc tgttgctctg atacgaagta agcgggggta aacgaatact
     8461 cttctctctt tcaatttgct gctctacttc acgaagttgg cattctgcac ctgcgatttg
     8521 ccctttccct gccagccatc gtggcgactc aataaagtat ttacctgaga gaagccaggc
     8581 taacagtatg ccaataccac ccaacagaaa cattatccgc caactaaaaa aagcgataac
     8641 caccacacct atcgccgcag acagcatggg cgaccagtta ccaacaaatg agagccgcgc
     8701 ggaccatttt ccacgcaccg tcgcggggat aaactccgta aatgaggcat agccaaccat
     8761 aatcagcgcc cccattcctg ttcccatcag gaagcgaaag aagatgagcc agtacatatc
     8821 agggacaaaa gcggcccctg ttgcagcaat accgacgatg agaagattta tgcgaaacgc
     8881 cctgcgccgc ccaaagtaat caccgatgaa cccaccggta agagagccga tgaaataacc
     8941 aaacattaat gccgaggtaa aggcggcatt gaggaaatta tttgaccatc cattgcttac
     9001 cagctttgct aagacaacat taccggagta actcaaaaaa cccgttaata acagactaaa
     9061 gcttattata ccaaatatac gataatgaaa ccgggccaaa ggcaagcggt ccagtcttgc
     9121 gccaatttga tcatattgtt ccattgggtg cctcggattt gctaaaaaag gtagcaattg
     9181 cggcaaattc acttgccgca tctttatcat cattttattt caaggcgcag gaacaaataa
     9241 tattgacaac gtgtgcttaa attaccacac cagctggata ttattgtctt tgcaaaatgc
     9301 tatccactct gcacccggtg atttatcagt tattatgtaa ttaatatgac taaaatcagc
     9361 taactggaca aaagctttgc gatcaaactt agagtgatca accaataacg caacttctgt
     9421 cgcctgacgg atcattgtct ttttgatttc agcttccgct tcattagagt ccagcgcgcc
     9481 gctgttaata tcaagacctt tacaactcat taccatgata tcgacatgat agcggctgat
     9541 gatctcttta gtaattcttc cttgcagtga aagtgtattt ttgtttagtt ccccacccgt
     9601 tgaaacgact ttaatttctg actgagccag aacatgaatc gcttccgccg agttggttag
     9661 caacgtcagg tcactacggt cctgtaacaa tttgagcaat tccattacgg tactactgga
     9721 atcggctgcc atggtggttt tattgtcgat aaagggtagt gccttgcgtg caataagctg
     9781 cttctcttca taaaacgatg aagcgcgctt ataaaaatgg atattctccg tcaacatcgc
     9841 tgtatttaaa acagcaccac cataggttct ggtcaaaaag ccttcatctt ccagcttctc
     9901 aagatcgcgg cgaatggttt cttcggttac ctgaaaaatc ccactcaaat ttgagactgt
     9961 cactttttta tcgttggcaa ccatttgctt aattgcctga atcctgtctt ttgccgccac
    10021 gattacaccc ctgtatcttt ttacatcaca ttagcgcgat tatcgcataa ccgatgttta
    10081 ctttcaaaac aacctgtttg aatcacagat tttcatcaca gttttcacag aaacagaggt
    10141 gaatcgtgtt gagtatttaa cgctccaggg cctctgccat ttccctcatc aatgttgcgt
    10201 ctgcatccga taagttgata atcagtgccg cgacattttc gcgtacctgt tccggtgcag
    10261 tagccccact aagaatggag attaaatcac tctgttttaa tatccacgcc agtgccagag
    10321 tgggaattgt gcactgataa cgagcacaaa gtggctgcca ctgttcaagc atatcaatca
    10381 ctttcagcat gttttcacgc tggaaccaga ctttatttgc ccgagcgccg cccggaacgt
    10441 aatcacgagt gatggtgccg gtcaacaatc cctgctctag cggggaataa acctgaacca
    10501 caatgccatt atcacgacat agcggcagca gttcgttttc cattgcccgg tcgaggatac
    10561 tgtatttcgc ctgaataata tccagttcac catattgcag atactcgcgg atatggtcag
    10621 catcgacgtt agcagcgcct atagcgcgaa ttttcccttc agccttcaac tcattaagca
    10681 ctgcgacagt ttcagcgatc ggcgtaaaaa atggcggcac cgactgccag tgcgtcatgt
    10741 agatatcgat gtaatcaata cccagacgtt gcaagctggc ttctacctct tcgcggatag
    10801 attccgggga aaggttttta tacaactgcc gatcgccaac tttgttgaat aaacttcctt
    10861 ttcgttccca gacaatgccg catttggttt ctactacaac ctgttcacgg ggcagttttt
    10921 ttaacgcctg accgacgata acttcactat tgccaaagtt atatcctggc gcagtatcaa
    10981 tcagattaat gccgcaacga tgggcttcaa gaatcgtatc aatacatatt tgccgatcga
    11041 gatcgccatt ccatgcagga ccgccgccaa tggcccatgt ccccaacccc attcgcgaaa
    11101 gcgtaatatc cgttgtgcct aaaggtatct ttttcatttg ccttcattat ccttcgtatt
    11161 cttccagcaa ttgttcgacc agctttctgt tttttacgcc ggtggtggca ccgacgctta
    11221 gaaccgagat agccgccgtt gcattggcaa agcgtgcgca ttcacgcaga tttttgcctt
    11281 ctaacagtgc cgcaataaaa cctgaagcaa agttatcgcc cgcgccaatg gtgtcgatgg
    11341 cggttattcc tgcgaccgcc ggcaccttca tcgtcatgtc accacgcttg ataaagcagc
    11401 cgtctttacc cgttttaatc accaccgttt ttacgccgca cgcaagaaag cagtcagcaa
    11461 tttcatccag tgtctctttc ccggtgagta attttgcctc ggcaaaatta ggaaacagat
    11521 aatcgacata actcaatgct tcgcaaatat catccagcgt ttcattcaac cgcggtttga
    11581 tcatatcggc acagataatc atctgccggg ctttagcttg cgtaaaaatt tctgttagcg
    11641 ctttaccatc caatagtgga ctgttgaaaa tactggccag tgataataat ttcgcctgag
    11701 aaaaccgcgc aaaatcaaca tcgtcaatat tcaatttcca cagactgcca ttacggtttg
    11761 tgacaaacgt ccgctcgcca tcctccgtca ccagtccaac gttaatagag gtatctatgc
    11821 tgacgtcctg cttcaggctt tgaatatcaa tattctcttt gcggcaatgg tcgagaataa
    11881 attgccctgc ggcatcttta ccaatacgac tcattaatgc tgtgcgatgg cccagacgag
    11941 aaataattgt tgcttcattt attgcatcac ccccggtggt cattgcgatt ctttcaagag
    12001 gataagaatc cacatcaaag atatttttac tgaccggttg caatggaata tcaacaatag
    12061 cggcacctat acaaataacg tcgagattat ccatatcatt attccgcttt gccatcagaa
    12121 ccgaacagtt taattttttc caatgcccgt tcttttacag ctttacgtac ttcacgttct
    12181 aaatgcagga aaggctgatc ctggttttct ttcactgcaa ccattgcggc ctgacagagc
    12241 tccgtatgaa tgttgatttt cgcgataccc aacgaaattg cagttttaat gtcggcatca
    12301 ctgatccccg atgcaccgtg caaaaccagc ggaacagaaa cggcatcgcg tacgcgtttg
    12361 acgacctcaa agttcaattg tggctctgac gtataaaccc catgctggtt gccgatggcg
    12421 acagccaatg aatcacagcc cgtacgttca acaaattcag ccgcctggta aggatcggta
    12481 tagtgatagc ccgccagcgc ttcctcataa acggtttcat taccgacatg ccctaattcc
    12541 gcctctaccg gaatacccag cggatggaag aaatcgacag cctctttggt taagcgaata
    12601 ttttcttcga aatcaaatgc ggaagcatca cgcattaatg aattcatacc atgagtccag
    12661 gcgttatgaa taatctccat actccgacca tgatcccaat gagttattac cggcaccgtt
    12721 gctttttgtg ccattgatac catcatgtga gagaaatctt caaatgaggt gttcccgaca
    12781 aaacctgtgc caaaagaaat aataaccggt gatttcgctt cttcggctgc gtcgataacg
    12841 cccatcaaca tttctgcatt ccatacgtta aaatgggcaa ttgcataatg tttatttgtg
    12901 gcattgtttt cccaatatct gatatctgcg agcatgttct gttctcccat attaattaat
    12961 cgctaa
//
LOCUS       DAZFZC010000122        12936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFZC010000122 DAZFZC010000000
VERSION     DAZFZC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.123"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(568..1659)
                     /gene="ychF"
                     /locus_tag="ACHKXP_004245"
     CDS             complement(568..1659)
                     /gene="ychF"
                     /locus_tag="ACHKXP_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO7649132.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1776..2360)
                     /gene="pth"
                     /locus_tag="ACHKXP_004246"
     CDS             complement(1776..2360)
                     /gene="pth"
                     /locus_tag="ACHKXP_004246"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO7649133.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINLDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2638..2916
                     /gene="ychH"
                     /locus_tag="ACHKXP_004247"
     CDS             2638..2916
                     /gene="ychH"
                     /locus_tag="ACHKXP_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO7649134.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2971..4650)
                     /gene="dauA"
                     /locus_tag="ACHKXP_004248"
     CDS             complement(2971..4650)
                     /gene="dauA"
                     /locus_tag="ACHKXP_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO7649135.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4775..5722)
                     /gene="prs"
                     /locus_tag="ACHKXP_004249"
     CDS             complement(4775..5722)
                     /gene="prs"
                     /locus_tag="ACHKXP_004249"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO7649136.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5873..6724)
                     /gene="ispE"
                     /locus_tag="ACHKXP_004250"
     CDS             complement(5873..6724)
                     /gene="ispE"
                     /locus_tag="ACHKXP_004250"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO7649137.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6724..7347)
                     /gene="lolB"
                     /locus_tag="ACHKXP_004251"
     CDS             complement(6724..7347)
                     /gene="lolB"
                     /locus_tag="ACHKXP_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO7649138.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7561..8817
                     /gene="hemA"
                     /locus_tag="ACHKXP_004252"
     CDS             7561..8817
                     /gene="hemA"
                     /locus_tag="ACHKXP_004252"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO7649139.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8859..9941
                     /gene="prfA"
                     /locus_tag="ACHKXP_004253"
     CDS             8859..9941
                     /gene="prfA"
                     /locus_tag="ACHKXP_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO7649140.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9941..10774
                     /gene="prmC"
                     /locus_tag="ACHKXP_004254"
     CDS             9941..10774
                     /gene="prmC"
                     /locus_tag="ACHKXP_004254"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7649141.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IRILQSDWFSALAGQQFTMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10771..11163
                     /gene="sirB2"
                     /locus_tag="ACHKXP_004255"
     CDS             10771..11163
                     /gene="sirB2"
                     /locus_tag="ACHKXP_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO7649142.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIFAFPLALVVLYIIIKLATTKVPLLG"
     gene            11167..11976
                     /gene="sirB1"
                     /locus_tag="ACHKXP_004256"
     CDS             11167..11976
                     /gene="sirB1"
                     /locus_tag="ACHKXP_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO7649143.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPNSEMIRAQINNIAHKHIVLH"
     gene            12012..12866
                     /gene="kdsA"
                     /locus_tag="ACHKXP_004257"
     CDS             12012..12866
                     /gene="kdsA"
                     /locus_tag="ACHKXP_004257"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO7649144.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3259 a         3242 c         3324 g         3111 t
ORIGIN      
        1 tatatcgagt tattccctaa gttgtcgtta cttattccaa ttcgttacta taaatatggc
       61 aaaaatatta caacaaacag cggttaaaaa atttggtgaa gattttttgt aataatccta
      121 attagttatg ggaatttcgc ttgatgtatc tgcataacaa aaacaatatg cgccattttt
      181 gcattactaa taagaaaaag caaaaaacat cgatattcat ttttggtcaa tgggttttct
      241 ggcctatcgt tgttattgtg ctactggtta tcattttcat cttgccagca tattggagcg
      301 tgatcaattt tgatcagctg tgaagggggc tgatgtgtag tgatacaaaa acttacgtta
      361 tatcactgct aatgagatat ccgtcatctg aacacttatg tctttaatta ttctcgtggt
      421 tcactatagg caataagcac aaaagtgtag gatgttacaa gaatgattag gactcggtga
      481 aatgaaaaat ccacgcaatt gcgtggattt tatatacttt tgcgctcttc atgagattta
      541 gcgaaacctc atgagacaat aaattaatta gacgttgaaa aggaagttca tcacatcgcc
      601 atctttcacg atgtaatctt taccttctgc acgcattttg cctgcttctt tcgcgccttg
      661 ttcacctttg taagtgatga aatcttcaaa cgagatggtt tgtgcacgga taaagccttt
      721 ttcaaaatca gtatggattt tgcccgctgc ctgcggcgcg gttgctccaa ccggaatggt
      781 ccatgcacgc acttctttca ccccagcggt gaagtaagtt tgcaggttca gcagtttata
      841 accggcacgg atcacacggt tcaggcccgg ctcttccagc ccaagctcct gcataaactc
      901 gtcacgttct tcgtcgtcca gttcggcaat gtctgcttca acagcagcac aaaccggaac
      961 cacaacagaa ccttctttcg ccgcgatttc acgcacctgg tcaagatatg ggttgttttc
     1021 aaaaccgtct tcgttgacgt tggcgatgta cattgttggt ttcagcgtca ggaagctcag
     1081 gtaacgaata gccgctttct cttcagcgct taaatccagc gcgcgcagca tacctgcatt
     1141 ttccaactgg ggcaggcatt tttccaggac cgccagctca gctttcgcgt ctttatcgcc
     1201 acctttggct ttcttctgta cgcgatgaat cgcacgttcg caggtgtcga ggtctgccag
     1261 cgccagttcg gtgttgataa cttcaatatc gtcagccggg ttaactttgc cggaaacgtg
     1321 aatgatgttg tcattttcaa agcagcgaac aacgtgaccg atcgcttcgg tttcacggat
     1381 gttggtcagg aactggttac ccagaccttc gcctttcgat gcgcctttta ccagaccggc
     1441 gatatcgaca aattccatgg tcgtgggaag cgtacgctgc ggttttacga tttcagccag
     1501 ttgatccagg cgaggatcag gcattggtac gacgcctgtg ttcggctcaa tggtgcagaa
     1561 tggaaagttg gccgcttcaa taccggcttt ggtcagcgcg ttgaacaggg tagatttccc
     1621 gacgttgggc aaaccgacga taccgcattt gaatcccatg atttaaatca ccttaatatc
     1681 ttaataatca acctgttatt gctaacagat tgcagaaatg gaaataactt tgcctattat
     1741 acacggcact cggcaaaaat gccgcagaca acgacttatt gcgctttaaa ggcgtgcaat
     1801 cggttcgttg ctttggtcaa gccatctgta aaccacattt cagtacaacg cgccgcttcg
     1861 tcaatggctt catcaattaa cttctgttca ctaacaggcg gtttgcctaa cacaaaaccg
     1921 acaactttat ttttatcgcc cggatgaccg attccgatgc gtaaacggtg aaagttaggg
     1981 ttattaccca atttactgat gatgtctttc agtccattgt gaccaccatg gccaccgccc
     2041 aatttaaatt tggcgacgcc aggaggcaga tccagttcgt cgtgggccac cagaatttcg
     2101 tccagattaa tgcggaaaaa actggccatc gccgcaacgg ctttgccgct gagattcata
     2161 aatgtagtcg ggactaacag gcggacatct tcgcctccaa gagtgactcg cgaagtataa
     2221 ccaaagaatt tagcctcttc gcgcagcgga gcgcgcaaac gctctgccag taagtcaacg
     2281 aaccaggcac cagcattatg tcgcgttgcg gcgtattcag caccggggtt cgccaggccg
     2341 acaatcaatt taatcgtcac gtttttttgt cctgagtgtg tacataactg gcgcgtagtt
     2401 tactggttgc ggccccgctt gacaaaaaac tgcgtatcaa atgcagataa cgtaataatt
     2461 gcctgagtgg actattagaa agtcaaggtg ttcaggcgtt tatttgtaaa gttttgttga
     2521 aataagggtt gtaattgtga tcacgcccgc acataaccca ctgggtgttg tctatacttt
     2581 acacataagg aagaggggta ttccctgtta caacccagaa agttccggag gtgacatatg
     2641 aaacgcaaaa acgcttcgtt actcggtaac gtgctcatgg ggttgggtct ggtggtaatg
     2701 gtggtcggcg tggggtattc aatcctcaac cagttaccac agtttaatat gccccagtat
     2761 ttcgcacatg gtgcagtgct aagtattttc gtcggtgcca ttctctggct ggcgggtgcc
     2821 cgtgttggcg ggcatgaaca ggtgtgcgac cgttactggt gggttcgcca ctatgacaaa
     2881 cgttgccgcc gtagcgataa tcgccgtcat agctaacaaa taatgcagtt tgctgaccag
     2941 tcagtttgcg ctgactggtc aatctcgtac ttataaatcc gccatcgccg cgcgacgatt
     3001 cgggaagaac gccaggcgtc ccgggatcgg ttgaatgcca gcgcgagcca tagtgcgcag
     3061 tggctggaat tccacgttgc acacgcgcag ttcacatccc tcgggcagac gcttcacaaa
     3121 acgctggaac gcatcaagac caccagcatc aagtaccgga acggcatccc acttcagaat
     3181 cacaatccgt ttgccttcaa gacgtgactc caggtccgtg aataagcctt cagcagcagc
     3241 aaaaaacagc gggccaataa cgcgcagaac caggacatcg tctggaacat ctacgactac
     3301 cggtgccagg cgagtcatac gtgcgatacg acgcataaac agcagcgatg ccagcacgat
     3361 ccccacgctg atggcaataa ccatatcaaa caacacggtc agcgacatgc acagcagcat
     3421 gacgatgatg tcatctttcg gcgcatgacg cagcaagtcg accactttgt gcgcttcact
     3481 catgttccac gccaccatca acagcagggc tgccatggcg gaaagcggca gccaggagag
     3541 cagcggtgcc agtaccagca gggcaagaat aaccagaata gagtggatca ccgccgagat
     3601 aggggacgtt gccccggcac ggacgttagc ggcagaacgc gcgatggcag ctgtagcggt
     3661 aataccacca aagaacggag cgataatatt ccccagtccc tgtccaacca gttcgctgtt
     3721 cgccttgtgt ttcgtcccgg tcataccatc cagcaccacg gcgcagagca gagattcgat
     3781 tgcgccgagc attgccattg agaatgccgc aggcagcagt gtgcgaatag aatcccaggt
     3841 tagcgtgaat tctgaattag gcagatccca cggcagcacc agttgcggca gcagttgcgg
     3901 aataccgtta ccctgagaac catcggccag gacgtagtgg aattgcgaac cgatggtagc
     3961 aacatgtccg ccgagcaggt taacaatccc catcaccgcg caaccagcca gcaaggccgg
     4021 aaggtgaccg ggtaaacgaa tgcccagacg cggccaaaaa acaagaatac ctagcgtcac
     4081 aatgccaatg gcagcatcac ccacattaat ggtcggcagc gccataaata atgcgccgac
     4141 tttttgtaga taatgttccg ggacatgggc catttgcaga ccgagaaaat ctttaatctg
     4201 catggtaccg atggtgatcc cgatacccga ggtgaaacct aaggtgacgg aaaccggaat
     4261 atactcaatc aggcgaccaa agcgtgccag acccatcaga atcaaaaaga tccccgacag
     4321 caaggtcgca accagcagtc ctgccagtcc aaactgttgc gacacgggat agagaattac
     4381 cacaaatgcc gcagtcggac cggaaacgct aaagcgtgac ccacccgtca gagcaatgac
     4441 aatccccgca acagctgcgg tatataaacc gtactggggt gccacaccac taccaatagc
     4501 caacgccatc gccagcggga tagcaataat cccgacggtt atcccggcaa tcaggtcacg
     4561 ggtaaaccgt gcggcagtat atttttcttt ccagcaagcg tcgatcagag cgcggaaagg
     4621 catcacatgt gaggaaaata ttttgttcac aataatgttt catccgtgag cgcatcatct
     4681 gtcaactaaa tggcaggtga aggaggcata ggtcatacaa atggatatta cagacaaaaa
     4741 aacccgccgc agcgggtctt tgagccgggt tcgattagtg ttcgaacatg gcagagatcg
     4801 attcttcgtt gctgatacga cgaatcgctt cggccagcat acctgacagg gtcagagtac
     4861 gcacgttcgg cagtgatttg atttcatcgc tcagcggaat ggtatcgcag acaacgactt
     4921 catcaattac agagttacgc aggttgttcg ccgcgttgcc agagaagatc gggtgagtcg
     4981 cgtacgcaaa tacacgttta gcaccacgtt ctttcagagc ttcagcagct ttacacagcg
     5041 taccgccagt gtcgatcata tcatcgacca gtacgcagtc acgacctgca acgtcaccga
     5101 tgatatgcat cacctgtgaa acgttcgcac gcggacgacg tttgtcgatg attgccatat
     5161 cggtatcgtt cagcagctta gcgatagcgc gggcacgcac aacgccgccg atgtccggag
     5221 aaaccacaat tgggttatcc agattcagct gcagcatgtc ttccagcagg atcgggctac
     5281 caaatacgtt atcaaccgga acgtcgaaga aaccctgaat ctgttcagcg tgcagatcca
     5341 ctgtcagcac acggtcaaca ccgacgctgg agaggaagtc tgcaaccact ttcgcagtga
     5401 ttggtacacg agcggaacgg acgcgacggt cctggcgcgc atagccaaag taggggataa
     5461 cagcggtgat acgacctgcg gaagcacgac gcagggcatc aaccataacg actaattcca
     5521 tcaggttgtc gttagtaggg gcacaagtgg actggatgat gaaaatatca ccaccgcgta
     5581 cattttcatt aatttgtacg ctgacttcgc catcgctaaa gcgacctaca gcggcgtcgc
     5641 cgagtgaagt gtacaggcgg ttggcaatac gttgtgctag ttccggggtg gcgttaccag
     5701 caaaaagctt catatcaggc acgagaagaa cctcaggcat gcgtccattg gtggaaagaa
     5761 tctgccgaaa actgtgcggg ccaggcatga tcctttccag gcggtgtatt aaagagcgcg
     5821 atgcaacgtc tggaacaagg tgacgttgtc accgaaactc agcttgcccg gcttaaagca
     5881 tggctctgtg caatggggaa agattagcgc ctttcgccac aaagccattg agccattccg
     5941 gggcttgctc tagcacctgg cgggcttcag actctgtatc aaattcagca aagacacagg
     6001 cccctgtccc agtcaggcgc gacggggcgt attctaacag ccaggaaagc accgcatcaa
     6061 cctcgcgaaa acgttttctt gcgataacct cgcaatcatt gctgaattca cattttagca
     6121 acgtttctat tgaccttttt ggcgtattgc gcgggagttc aggatcttta aaaatcaccg
     6181 gagtcggaat acttacacca gggtgcgcca ccagatacca cttctctggc ggatccaccg
     6241 gcgttagtat ttcaccaacg ccttcggcaa acgcggcatg cccccgaaca aagacaggaa
     6301 catctgcgcc cagcgtcagc cccatttccg ccagctcatc catgcttagc ccgcattgcc
     6361 agagatgatt taatgccacc aggaccgtcg cggcattgga tgaaccaccg ccgagaccgc
     6421 cgcccatcgg caaacgctta tcaatgctga tatccgcacc gcttcccgtc ggaagacgcc
     6481 cgctgtctgc cgcagttttc atcaacaacc gcgctgcgcg aacgatcagg ttatcttcat
     6541 gttccacgcc ttcaacgggc gttaacagac gaatatcccc atcgtcacga agctcaatgc
     6601 tgatggtgtc gccgtaatca agaaactgaa acagcgtttg cagcgtgtgg taaccatccg
     6661 cacgctgacc ggtaatgtat aaaaacagat taagttttgc cggagagggc cactgtgtcc
     6721 gcattatttc actatccagt tatccatttt taacttgatg cgttgaccac cgtcggtgag
     6781 ttccatattg gctggcatcg caggttgcgt tttggtgtca taaccaccat aaacaacctt
     6841 ccagtttttg ccattctggc tgtaggtaat ttcgctcagg cggtactggt cgtccagttt
     6901 gtagtcggtt gcatcacccg gtaaacctaa aatccactgg cgcaagctgt tgagcggaat
     6961 tggcattccg gtcaatttgc caatcatctc ttcggcgtca tcggcggtat aacgctgacc
     7021 tttattgtcg actaactgca cgttacccgg ttgagcattc agctccagtt ccgtgctgcc
     7081 caatgggtta gtgagcagca gacggtagcg atcctggccg gtttgctgcc agaaaaagcg
     7141 ggcgtacact ttttgttggt cagaaatata agcgaacgcg ccgcgagtct gatactgatt
     7201 aagattgcgc acgtcttgct gatgctgacg ccattgtggc gaatccgggc ttttgccagg
     7261 acctttgggc gtggtaacgg aacaggcagt gagcacaaga gcagccagcg gtagcaggcg
     7321 gataagacga aaatcgggca ggggcatagt gatgacaagt ccttgagata cgttgcagtt
     7381 ataaccctta atgctagcgt taccgtccgc tatcgtctat gttcaagttg tcttaattgc
     7441 cagaatctaa cggctttcgg caattactcc aaaagggggc gctctctttt attgatctta
     7501 cgcatcctgt atgatgcaag cagactaacc ctatcaacgt tggtattatt tcccgcagac
     7561 atgacccttt tagcactcgg tatcaaccat aaaacggcac ctgtatcgct gcgagaacgt
     7621 gtatcgtttt cgccggataa gctcgatcag gcgcttgaca gcctgcttgc gcagccgatg
     7681 gtgcagggcg gcgtggtgct gtcgacgtgc aaccgcacgg aactttatct tagcgttgaa
     7741 gagcaggata acctgcaaga ggcgttaatc cgctggcttt gcgattatca caatcttaat
     7801 gaagaagatc tgcgtaaaag cctctactgg catcaggata acgacgcggt tagccattta
     7861 atgcgtgttg ccagcggcct ggattcattg gttcttgggg agccgcagat cctcggtcag
     7921 gttaaaaaag cgtttgccga ttcgcaaaaa ggccatatga aggccagcga actggaacgc
     7981 atgttccaga aatctttctc tgtagcgaaa cgcgttcgca ctgaaacaga tatcggtgcc
     8041 agcgctgtgt ctgtcgcttt tgcggcttgt acgctggcgc ggcagatctt tgaatcgctc
     8101 tctacggtca cagtgttgct ggtaggcgcg ggcgaaacca tcgagctggt agcgcgtcat
     8161 ctgcgcgaac ataaagtaca gaagatgatt atcgccaacc gcactcgcga acgtgcccaa
     8221 atactggcag atgaagttgg cgcggaagtg attgccctga gtgagatcga cgaacgtctg
     8281 cgcgaagccg atatcatcat cagttccacc gccagcccgt taccgattat cgggaaaggc
     8341 atggtggagc gcgcattaaa aagccgtcgc aaccaaccaa tgctgttggt ggatattgcc
     8401 gttccgcgcg atgttgagcc ggaagttggc aaactggcga atgcttatct ttatagcgtg
     8461 gacgatctgc aaagcatcat ttcgcacaac ctggcgcagc gtaaagccgc agcggttgag
     8521 gcggaaacta ttgtcgctca ggaaaccagc gaatttatgg cgtggctgcg agcacaaagc
     8581 gccagcgaaa ccattcgcga gtatcgcagc caggcagagc aagttcgcga tgagttaacc
     8641 gccaaagcgt tagcggccct tgagcagggc ggcgacgcgc aagccattat gcaggatctg
     8701 gcatggaaac tgactaaccg cttgatccat gcgccaacga aatcacttca acaggccgcc
     8761 cgtgacgggg ataacgaacg cctgaatatt ctgcgcgaca gcctcgggct ggagtagcag
     8821 tacatcattt tcttttttta cagggtgcat ttacgcctat gaagccttct atcgttgcca
     8881 aactggaagc cctgcatgaa cgccatgaag aagttcaggc gttgctgggt gacgcgcaaa
     8941 ctatcgccga ccaggaacgt tttcgcgcat tatcacgcga atatgcgcag ttaagtgatg
     9001 tttcgcgctg ttttaccgac tggcaacagg ttcaggaaga tatcgaaacc gcacagatga
     9061 tgctcgatga tcctgaaatg cgtgagatgg cgcaggatga actgcgcgaa gctaaagaaa
     9121 aaagcgagca actggaacag caattacagg ttctgttact accaaaagat cctgatgacg
     9181 aacgtaacgc cttcctcgaa gtccgtgccg gaaccggcgg cgacgaagcg gcgctgttcg
     9241 caggcgatct gttccgtatg tacagccgtt atgccgaagc ccgccgctgg cgggtagaaa
     9301 tcatgagcgc cagcgagggt gaacatggtg gttataaaga gatcatcgcc aaaattagcg
     9361 gtgatggtgt gtatggtcgt ctgaaatttg aatctggcgg tcatcgcgtg cagcgtgttc
     9421 ctgctacgga atcgcagggg cgtattcata cttctgcttg taccgttgcg gtaatgccag
     9481 agctgcctga cgcagaactg ccggacatca acccagcaga tttacgcatt gatactttcc
     9541 gctcgtcagg ggcgggtggt cagcacgtta acaccaccga ttcggcaatt cgtattactc
     9601 acttgccgac cgggattgtt gttgaatgtc aggacgaacg ttcacaacat aaaaacaaag
     9661 ctaaagcact ttctgtactc ggtgctcgca tccacgctgc tgaaatggca aaacgtcaac
     9721 aggccgaagc gtctacccgt cgtaacctgc tggggagtgg cgatcgcagc gaccgtaacc
     9781 gtacttacaa cttcccacag gggcgcgtta ccgatcaccg catcaacctg acgctctacc
     9841 gcctggatga agtgatggaa ggtaagctgg atatgctgat tgaaccgatt atccaggaac
     9901 atcaggccga ccaactggcg gcgttgtccg agcaggaata atggaatatc aacactggtt
     9961 acgtgaagca ataagccaac ttcaggcgag cgaaagcccg cggcgtgatg ctgaaatcct
    10021 gctggaacat gttaccggca aagggcgtac ttttatcctc gcctttggtg aaacgcagct
    10081 gactgacgaa caatgtcagc aacttgatgc gctactgaca cgtcgtcgcg atggtgaacc
    10141 cattgctcat ttaaccgggg tgcgagaatt ctggtcgttg ccgttatttg tttcgccagc
    10201 gaccttaatt ccgcgcccgg atacggagtg tctggtggag caggcactgg cgcggttgcc
    10261 tgaacaacct tgccgtattc tcgatctcgg gacgggtacc ggggcgattg cgcttgcgct
    10321 ggctagcgag cgcccggact gcgaaattac cgctgtagat cgtatgcccg atgctgtcgc
    10381 tctggcacaa cgtaatgccc agcatctggc gatcaaaaat atccgcattc tgcaaagcga
    10441 ctggtttagt gcgctggccg ggcagcagtt tacgatgatt gtcagcaatc cgccgtatat
    10501 tgacgagcag gacccacatc ttcaacaagg cgatgtccgc tttgagccgc tcactgcgct
    10561 ggttgcggca gacagtggaa tggcagacat cgtgcatatc atcgaacagt cgcgtaacgc
    10621 gctggtatcc ggcggctttc tgcttctgga acatggctgg cagcagggcg aagcggtgcg
    10681 acaagcattt atcctcgcgg ggtatcatga cgtcgaaacc tgccgtgact atggtgataa
    10741 cgagcgcgta acgctcggcc gctattatca atgacaagtt tttctacact gcttagtgtt
    10801 catcttatta gtatcgcgct ttctgttggg ctattaacct tacgtttctg gctacgttat
    10861 cagaagcatc cacaggcatt tgctcgctgg acgcgcattg tgccgccggt tgtcgatacg
    10921 ctgttattgt taagcggcat tgcgttgatg gctaaagcgc acatcctgcc attttccggg
    10981 caggcacagt ggctgactga aaagctgttt ggagttatca tttatatcgt tttgggtttt
    11041 attgcactcg attatcgtcg tatgcacagt cagcaggcgc gcatttttgc cttcccgctg
    11101 gcgttggtgg ttctgtacat catcattaaa ctcgccacca caaaagtacc gttactgggg
    11161 taagtcatga gatcgttagc tgatttcgaa tttaataaag cgccattgtg cgaaggcatg
    11221 atcctggctt gcgaagcaat ccgccgcgat tttccctcgc aagatgttta cgacgaactg
    11281 gagcgtctcg ttagtctggc gaaggaagaa atcagccagc ttctgccttt agaagagcag
    11341 ctggaaaaac tgatcgcgct gttttacggc gactggggat ttaaagcctc acgcggtgtt
    11401 tatcgtcttt ccgatgcatt atggctggac caggtgttaa agaatcgaca gggcagtgcg
    11461 gtatcattag gtgcggtttt attatgggtc gcgaatcgtc tcgatttgcc gctgctgccg
    11521 gtgattttcc ctacgcagct gatattgcgc attgaatgtc cggatggcga aatttggctg
    11581 attaatcctt ttaacggtga atcgttaagc gaacatatgc tggacgtatg gttaaaggga
    11641 aatatcagcc cgtcggcgga actgttttat gaagaccttg atgaagctga taacattgag
    11701 gtaatccgca aattgctgga tacactcaaa gcctcgttga tggaagaaaa tcagatggag
    11761 ctggcgttac gcaccagcga agctttatta caattcaacc ctgaagatcc ctatgaaatt
    11821 cgcgatcgcg ggttgattta tgcgcaactg gattgcgaac acgttgcgtt gaacgattta
    11881 agttatttcg ttgaacagtg tccggaagac ccgaacagcg aaatgatccg tgcgcaaata
    11941 aataacatcg cgcataaaca tattgtgctg cattaattaa tcgacatttt actcaagatt
    12001 aaggcgatcc tatgaaacaa aaagtggtta gcattggcga catcaacgta gcaaatgacc
    12061 tgccgttcgt actgtttggc ggtatgaacg tgctggaatc tcgcgatctg gcgatgcgca
    12121 tttgcgagca ctacgtaacc gtgacccaga aactgggtat cccttacgtg ttcaaagcct
    12181 cttttgacaa agccaaccgc tcctccatcc actcttatcg tggaccgggc ctggaagaag
    12241 ggatgaaaat cttccaggag ttgaagcaga cttttggcgt gaaaattatc accgacgttc
    12301 acgaaccaag tcaggcacag cccgttgctg atgtcgtgga tgtgattcag ttgccggcgt
    12361 ttcttgctcg ccagactgac ctggttgaag ccatggcgaa aaccggtgcg gtaattaacg
    12421 tcaagaaacc acagtttgtc agcccgggac agatgggtaa tatcgttgat aaattcaaag
    12481 aaggcggcaa cgaaaaagtg attctttgcg atcgcggtgc taacttcggc tatgacaacc
    12541 tggttgtcga tatgctgggc ttcagcatta tgaagaaagt gtctggtaac tcgccggtga
    12601 ttttcgacgt gacccacgca ctgcaatgcc gcgatccttt tggcgcagct tccggcggtc
    12661 gtcgtgctca ggtggctgag ctggcacgag ccggtatggc ggtaggtctg gcagggctgt
    12721 ttattgaagc gcatccggat ccggaacatg cgaaatgtga tggtccatcc gcgctgccgc
    12781 tggctaaact ggaaccgttc ctcaagcaga tgaaagcgat tgatgatctg gtgaaaggtt
    12841 tcgaagaact ggataccagc aagtaatctt tttgtttgaa aaataaagtc ttagcgttgt
    12901 gcgttaagac ttttttatgg gtgccggata caaaaa
//
LOCUS       DAZFZC010000123        12872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFZC010000123 DAZFZC010000000
VERSION     DAZFZC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.124"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            232..1746
                     /gene="caiT"
                     /locus_tag="ACHKXP_004258"
     CDS             232..1746
                     /gene="caiT"
                     /locus_tag="ACHKXP_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO7649145.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1776..2918
                     /gene="caiA"
                     /locus_tag="ACHKXP_004259"
     CDS             1776..2918
                     /gene="caiA"
                     /locus_tag="ACHKXP_004259"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO7649146.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3047..4264
                     /gene="caiB"
                     /locus_tag="ACHKXP_004260"
     CDS             3047..4264
                     /gene="caiB"
                     /locus_tag="ACHKXP_004260"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO7649147.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLATHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4338..5891
                     /gene="caiC"
                     /locus_tag="ACHKXP_004261"
     CDS             4338..5891
                     /gene="caiC"
                     /locus_tag="ACHKXP_004261"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO7649148.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNCPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            6000..6785
                     /gene="caiD"
                     /locus_tag="ACHKXP_004262"
     CDS             6000..6785
                     /gene="caiD"
                     /locus_tag="ACHKXP_004262"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO7649149.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6791..7381
                     /gene="caiE"
                     /locus_tag="ACHKXP_004263"
     CDS             6791..7381
                     /gene="caiE"
                     /locus_tag="ACHKXP_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO7649150.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRYHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7590..7985)
                     /gene="caiF"
                     /locus_tag="ACHKXP_004264"
     CDS             complement(7590..7985)
                     /gene="caiF"
                     /locus_tag="ACHKXP_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO7649151.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8247..11468)
                     /gene="carB"
                     /locus_tag="ACHKXP_004265"
     CDS             complement(8247..11468)
                     /gene="carB"
                     /locus_tag="ACHKXP_004265"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO7649152.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11486..12634)
                     /gene="carA"
                     /locus_tag="ACHKXP_004266"
     CDS             complement(11486..12634)
                     /gene="carA"
                     /locus_tag="ACHKXP_004266"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO7649153.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3008 a         3349 c         3416 g         3099 t
ORIGIN      
        1 ctcacaatag agcataacaa actgattatt aatcaaaatt aacgaaaaaa cgccctctat
       61 tcacccgaaa caaaaatgtg ataccaatca cagaatacag cttattgaat acccattatg
      121 agttagccat taacgcgtcc acgaggttaa taataattat attaaatgtt aacaaaaata
      181 aaacaaacgg gaacgcaaaa taaatattcg ttttcacagt ggaattaact catgaagaat
      241 gaaaagagaa aaacgggaat agaaccgaag gttttctttc cgccgttaat aatcgtcggc
      301 atactttgtt ggcttacagt cagagatctg gatgcagcga atgtcgttat taatgctgta
      361 ttcagttacg tcaccaatgt atggggatgg gcatttgaat ggtatatggt ggtgatgctt
      421 ttcggttggt tctggctggt gtttggcccg tatgccaaaa agcgtttagg taacgaaccg
      481 ccagaattta gcaccgccag ttggatcttt atgatgttcg cctcctgtac gtctgctgcc
      541 gtactgttct ggggatcgat tgagatctac tactacatct ccaccccgcc gtttggctta
      601 gaaccgaact cgacaggggc gaaagagttg gggctggctt acagcttgtt ccactgggga
      661 cctctgccgt gggccactta cagcttcctt tcagtcgcct tcgcttactt cttctttgtc
      721 cgcaaaatgg aagtgattcg ccccagctcg acactggtgc cgctggtagg tgaaaaacac
      781 gccaaagggt tgttcggcac tatcgtcgac aacttctatc tcgtcgcctt gatcttcgcg
      841 atgggtacca gtctgggcct tgccacgccg ctggtgaccg agtgtatgca atggttgttt
      901 ggcattccgc ataccctgca actggacgct atcatcatta cctgctggat tatcctcaac
      961 gccatttgcg tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt
     1021 tacctgagct tcctgatgct gggttgggtg ttcattgtca gcggtgccag cttcatcatg
     1081 aactacttca ccgattcggt ggggatgttg ctgatgtatc tgccgcgcat gttgttctat
     1141 accgatccca tcgctaaagg cggcttcccg cagggctgga ccgtgttcta ctgggcatgg
     1201 tgggtgattt atgctatcca gatgagtatc ttcctcgccc gcatctcccg tggtcgtact
     1261 gtgcgtgaac tgtgcttcgg catggtgctg gggctgacag cgtcaacctg gatcctgtgg
     1321 actgtactcg gtagtaacac tctgctgttg atagataaaa acatcatcaa cattccaaat
     1381 ctgatcgaac agtacggtgt ggcgcgcgcc atcattgaaa cctgggccgc tctgccactc
     1441 agcaccgcca ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt tacgctggtt
     1501 aacgcctgct cttataccct ggcgatgtcc acttgccgcg aagtacgcga tggtgaagaa
     1561 ccaccgctgc tggtgcgtat cggctggtca attctggttg gcattatcgg tattgttctg
     1621 ctggcgctcg gcggcctgaa accgattcaa accgccatta tcgccggagg atgcccgctg
     1681 ttcttcgtca acattatggt gacgctctcc tttattaaag acgcgaaaca gaactggaaa
     1741 gattaattaa ccccaaaata tcaagaggtt gaaagatgga ttttaattta aatgatgagc
     1801 aggaactgtt tgtcgccggt atccgcgaac tgatggccag cgaaaactgg gaggcctatt
     1861 ttgccgagtg cgaccgtgac agcgtctacc cggaacgttt tgtcaaagca ctggcggata
     1921 tgggtatcga cagtctgctg atccctgaag agcacggtgg tctggacgcg gggtttgtta
     1981 ctctcgccgc cgtgtggatg gagctgggac gtctgggggc accaacctat gtactgtacc
     2041 agttgccggg cgggttcaat accttcctgc gcgaaggcac acaagagcag atcgacaaga
     2101 ttatggcttt ccgcggcacc ggtaagcaga tgtggaactc agcgattacc gaaccgggcg
     2161 cgggctccga cgtgggtagc ctgaaaacga cttatacccg tagaaatggt aagatttatc
     2221 ttaatggtag taagtgtttt attaccagta gcgcctacac cccgtacatc gtggtgatgg
     2281 cgcgcgacgg ggcttctccg gacaaacctg tctacaccga atggtttgtt gatatgagca
     2341 aaccgggcat caaagtgacc aaacttgaga agctcggtct gcgtatggat agctgctgtg
     2401 aaatcacctt tgacgatgtg gaactggacg agaaagacat gttcggtcgg gaaggtaacg
     2461 gctttaaccg cgtcaaagaa gagttcgacc atgaacgttt cctggtagcc ctgaccaact
     2521 acggtacggc gatgtgcgcc tttgaagatg cggcgcgcta cgccaaccag cgcgtgcagt
     2581 ttggcgaggc tattggtcgt ttccagttga ttcaggaaaa attcgcccac atggcgatca
     2641 aattaaactc catgaaaaac atgctgtatg aagcagcgtg gaaagcagac aacggcacca
     2701 tcacctctgg cgatgcagcg atgtgcaaat acttctgcgc caatgcggca tttgaagtgg
     2761 tggatagcgc aatgcaggtg ctgggcggtg tcgggattgc gggcaaccac cgcatcagcc
     2821 gcttctggcg tgacctgcgt gtagaccgcg tttccggagg atctgacgaa atgcagatcc
     2881 tgacgctggg tcgtgcggtg ctgaagcaat accgctaagt ccgtgcgctg cctgatgcga
     2941 cgctgacgcg tcttatcagg cctacgggag cacgaatgta ggccggataa ggcgtttacg
     3001 ccgcatccgg cagtcatgcg ctacataaat ttttcaggag ctaataatgg atcatctacc
     3061 catgccgaaa ttcgggccgt tggccggatt gcgcgttgtc ttctccggta tcgaaatcgc
     3121 cgggccgttc gccgggcaaa tgttcgcaga atggggtgcg gaagttatct ggatcgagaa
     3181 cgtcgcctgg gccgacacca ttcgcgttca accgaactac ccgcagctct cccgccgcaa
     3241 tttgcacgcg ctgtcgttaa atattttcaa agatgaaggc cgcgaagcgt ttctgaaatt
     3301 aatggaaacc accgatatct tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg
     3361 cattaccgat gaagtactgt ggcagcacaa cccgaaactg gttatcgctc acctgtccgg
     3421 ttttggtcag tacggcaccg aggagtacac caatcttccg gcctataaca ccatcgccca
     3481 ggccttcagt ggttacctga ttcagaacgg tgatgttgac cagccaatgc ctgccttccc
     3541 gtataccgcc gattactttt ctggcctgac cgccaccacg gcggcgctgg cagcactgca
     3601 taaagcgcgt gaaaccggta aaggcgaaag tatcgacatc gccatgtatg aagtgatgct
     3661 gcgtatgggc cagtacttca tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat
     3721 gagcaaaggt aaagatccct actacgccgg ttgcggtctg tataaatgtg ccgacggcta
     3781 catcgtgatg gaactggtgg gcattaccca aattgaagag tgctttaaag atattggcct
     3841 cgcacatctg cttggcacgc cagaaatccc ggaaggcact cagcttatcc accgtatcga
     3901 atgcccttac ggcccactgg ttgaagagaa actcgatgcc tggctggcga cacataccat
     3961 cgcggaagta aaagaacgct ttgctgaact gaatatcgcc tgcgccaaag tgctgaccgt
     4021 accggaactg gaaagcaatc cacagtatgt ggctcgcgaa tcaatcactc agtggcaaac
     4081 gatggatggt cgcacctgca aagggccgaa catcatgccg aaattcaaaa ataaccccgg
     4141 acaaatctgg cgcggaatgc cctcacatgg catggacacg gctgccattt tgaaaaatat
     4201 cggctacagc gaaaacgaca ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga
     4261 ctaaacgcgt ggcgtcagtc ggcttgcctg actagcgccc gcccaataac aaacgaacaa
     4321 aaatggatag aggtgcaatg gatatcattg gcggacaaca tctacgtcaa atgtgggacg
     4381 atcttgcgga cgtttacggt cataaaacgg cgctgatttg tgaatccagc ggcggagtcg
     4441 ttaaccggta tagttatctt gagttaaatc aggagattaa ccgcacggca aacctgtttt
     4501 atacgctggg gattcgcaaa ggcgacaagg ttgcactaca tctcgacaac tgcccggaat
     4561 ttatcttttg ctggttcggg ctggcaaaaa ttggcgcgat tatggtgccg attaacgccc
     4621 gcctgttacg cgaggaaagc gcgtggatcc tgcaaaatag ccaggcgtgc ctgctggtaa
     4681 ccagtgcgca attctatcct atgtatcaac agattcagca ggaagatgcc actcaattgc
     4741 ggcacatttg cctgacagat gtggcacttc ccgctgatga tggcgtgagt tcgtttactc
     4801 aactgaaaaa tcaacaacct gccaccttgt gctatgcacc gccgctatcg actgacgata
     4861 cggcggaaat tctcttcacc tccggcacca cctcccgacc gaaaggtgtg gtgattaccc
     4921 attacaacct gcgcttcgct ggatattact ccgcctggca gtgtgcactg cgtgacgatg
     4981 acgtctacct gacggtaatg cctgcgtttc atatcgattg ccagtgtact gcggcgatgg
     5041 cggcgttttc tgccggggcc acctttgtgc tggtcgagaa atacagcgcc cgcgccttct
     5101 ggggacaggt acagaagtac cgcgccaccg ttaccgaatg tattccgatg atgatccgta
     5161 ccttgatggt gcagccgcct tcagcgaacg atcggcaaca ccgcctgcgg gaagtgatgt
     5221 tttatctcaa cttgtcggag caggaaaaag atgcgttttg tgaacgcttc ggcgttcgct
     5281 tgctgacgtc ttatgggatg acggaaacca ttgtgggcat tatcggcgat cgccctggcg
     5341 ataaacgacg ctggccgtcg attggtcgag cggggttttg ctacgaagcg gagatccgcg
     5401 acgatcacaa ttgcccgctc ccggcaggtg agatcggtga aatctgtatt aaaggcgtac
     5461 cagggaaaac catcttcaaa gagtattttc tcaacccgaa agccactgcg aaagtgctgg
     5521 aagctgatgg ctggctgcat accggcgata ccggataccg cgacgaagaa ggcttttttt
     5581 atttcgtcga tcgccgctgc aatatgatca aacgcggtgg cgagaatgtc tcctgcgtgg
     5641 agctggaaaa tatcatcgcc acccatccga aaattcagga catcgtggtt gtgggtatta
     5701 aagattcgat tcgcgatgaa gccatcaaag catttgtggt gctgaatgaa ggtgaaacat
     5761 tgagcgaaga ggaatttttt cgcttctgcg aacaaaatat ggcgaaattt aaagtgccct
     5821 cttatctgga gatcagaaaa gatctgccac gtaattgctc ggggaaaata attagaaaga
     5881 atctgaaata aatgaaacgg cagggaacta ccctgccagc taataatcac gctctaaaac
     5941 aatatgcatt ttttgcagga atgttgtcgt ccctgaaaaa gcaaaaatgg agaaaaggaa
     6001 tgagtgaatc attacatctg acccgcaatg gatcaattct ggaaattacc cttgatcgcc
     6061 caaaagcgaa tgctattgat gcaaaaacca gctttgaaat gggcgaagta tttctaaatt
     6121 tccgtgacga tccgcaatta cgtgtcgcca ttattaccgg tgccggagag aaattctttt
     6181 ccgcgggctg ggatttaaaa gcggcagcag aaggcgaagc accggatgct gactttggcc
     6241 cgggtggttt tgcgggatta accgaaattt tcaatctcga caaaccagtt atcgcagctg
     6301 tgaacggcta cgcctttggc ggcggtttcg aactggcgct ggcggcagat tttattgttt
     6361 gtgccgataa cgccagcttc gccctgccgg aagccaaact gggcatcgtt cctgacagcg
     6421 gcggtgtgct gcgtctgccg aagatcctgc cgcctgccat cgtcaatgaa atggtgatga
     6481 ccggcagacg aatgggcgca gaagaggcgc tgcgttgggg gatagtcaac cgcgtggtta
     6541 gccaggcgga actgatggat aacgcccgcg aactggcgca gcagctggtt aacagcgctc
     6601 cgctggcgat tgcggcgctg aaagagatct accgcaccac cagcgaaatg ccggtagaag
     6661 aagcgtatcg ctatattcgc agcggcgtgt tgaaacacta tccgtcggtt ctgcattcgg
     6721 aggatgccat tgaagggccg ctggcgtttg ccgagaagcg cgatccggtg tggaaaggac
     6781 gttaacaacc gtgagctatt acgcctttga ggggttaatt ccggtggttc acccgacggc
     6841 gtttgtccat cccagtgccg tcttgattgg cgatgtgatt gtgggagccg gtgtctacat
     6901 cggcccactc gcctcactgc gtggtgacta cgggcggttg atcgtgcaag cgggagccaa
     6961 tattcaggat ggctgcatta tgcatggcta ctgcgacact gacaccattg ttggggaaaa
     7021 cggccatatc gggcacggag cgatcctgca tggttgtgtg attggtcgcg atgcattggt
     7081 cgggatgaac agcgtgatta tggatggcgc ggtcattggc gaagagagca ttgttgccgc
     7141 catgagcttt gtcaaagcgg gctttagcgg cgagaaacgc cagttgttga tgggtacgcc
     7201 agcccgcgct gtacgcagtg ttagtgacga cgagttacac tggaaacggt tgaataccaa
     7261 agagtatcag gatcttgtcg ggcgctatca tgcatcgtta catgaaacgc agccgttgag
     7321 gcaaatggag gaaaatcgcc cccgtttgca ggggacaacg gatgtgacgc ctaaacggtg
     7381 atcttgccgg atgcggcgta aacgccttat ccaactctgc tggagagcaa ccgtaggccg
     7441 gataagatgc gccagcatcg catccggcga tagctgtcgg atgcggcata aacgccttat
     7501 ccaacccggc tggagagcaa ccgtaagccg gataagatgc gccagcatcg catccggcga
     7561 ccaggtcgca cggtatcatc gaaatgacat taacgacgca tactctttga caacatcatc
     7621 tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg cggtgtcttt
     7681 cttacgccga ctaatttttc ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg
     7741 atatcgacca ctttaaccag tcgctgacac tggcatcccc gcccttccag cttattaggg
     7801 atcatcttaa cttcgcagct tatttctgtg acttccgaca gaatataagt cagggtatta
     7861 accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat cacccaccga
     7921 ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac atatccttca
     7981 cacatacgaa ctccagtttc acaaattaaa agtctggcta tgactcgttt tgaagcaatt
     8041 aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac cgtcgataag
     8101 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
     8161 gggcaaaaaa aaacggtcct catcagagaa ccgtattagt tattcgatca aattagcgga
     8221 tgaaaaatat ctgccatgac acgctattat ttgatctgtg cgtgcatttc ctgcaccgaa
     8281 attacttttt cagtcgcatc ggcattcagc gccatcgcgg tggcaaagcc gccgttcagg
     8341 gtggtgtcgt aatgcacttt atattgcagc gcactgcgac gaatcacgcg ggagtcttca
     8401 atcgcacgac ggcctgaggt ggtgttgatg atgtaggtat attcgccatt cttgatacgg
     8461 tcctgaatgt gcggacggcc ttcatgcacc ttgtttacca gacgcgggtt gatacctgct
     8521 tcgcccagca caatcgccgt gccgtgggtc gcatccagct cgaagccctg tttcagcagt
     8581 tttgccgcca ggtccaccac gcgttcttta tcgccttcgc gcacggaaag cagcgcacga
     8641 ccgtgtttct tcatggtgga gttgctgccc agctgcgctt tggcaaacgc ttcagcgaag
     8701 gtgcggccca cgcccatgac ttccccggta gagcgcattt ctggccctaa cagcgggtca
     8761 acgcccggga atttattgaa cggcagcacc acttctttca ccgagtagta cggcgggata
     8821 acttctttgg ttacgccctg ctcagccagc gatttgccag ccatcacgcg cgccgccact
     8881 tttgccagcg gtacgccggt ggctttggag acgaacggaa cggtacgcgc cgcacgcggg
     8941 ttaacttcaa tcaggtagac ttcgttgttt ttcaccgcaa actgcacgtt catcaggccg
     9001 cgcacctgca attcgaaggc cagtttctgc acctgctggc gcatcacatc ctgaatttcc
     9061 tgacttaaag tgtaggctgg cagcgaacat gcggagtcac cggagtgcac gcccgcctgc
     9121 tcaatatgct ccatgatgcc gccaatcagc accatttcgc cgtcgcagat ggcgtccaca
     9181 tccacttcta ccgcgtcatc gaggaagtgg tccagcaaca ctggcgcatc gttagacacg
     9241 ctgaccgccg tctggaagta gcgacgcagg tctgcttcgt catagacgat ttccatcgcc
     9301 cgaccgccga gaacgtaaga cggacgtacc accagcgggt agccaatctc tttcgccttc
     9361 tctaccgcca tttcgatagc ggtaacggtg gcgttcgccg gttgcttcag tttcaggcgg
     9421 tcaaccgcgt gctggaagcg ttcacggtct tccgcacggt cgatagcatc cgggctggtg
     9481 ccgataaccg gtacgccagc ggcttccagt gcacgcgcca gtttcagcgg ggtctgaccg
     9541 ccgtactgaa cgataacgcc tttcggcttc tcaatacgca cgatttccag cacatcttcc
     9601 agggttaccg gctcgaagta gaggcggtcg gaagtgtcgt agtcggtgga gacggtttcc
     9661 gggttacagt taaccataat ggtttcgtaa ccgtcttcgc gcagcgccag cgaggcgtgt
     9721 acacagcagt agtcgaattc gataccctga ccgatacggt tcgggccgcc gccgagaacc
     9781 atgatttttt cacggtcggt agacggattc gcttcgcact cttcttcata agtggagtac
     9841 atgtaagcgg tgtcggtggc gaactctgcc gcacaggtat ccacgcgctt ataaaccggg
     9901 tgcaggtcat actggtcgcg cagcttgcgg atttccgctt cgcgcacgcc cgccagtttt
     9961 gccagacgcg catcggcaaa gcctttgcgt ttcagctggc gcaggaattc agcgttcagg
    10021 ccagtgatgc ccacttccgc tactttctct tccagacgca ccagttcttc aatctgtacc
    10081 aggaaccagc ggtcaatatt ggtcaggttg aagacgccgt ccacagacag gcccgcacgg
    10141 aacgcatcgg cgatgtacca gatacgatct gcgcctgcgt ctttcagttc gcgacggatt
    10201 ttggttaacg cttccgggtc atccaggctc actttcgggt cgaatccagt cgcaccgact
    10261 tccaggccgc gcagcgcttt ttgcagggat tcctgctgcg tgcgaccaat cgccatcact
    10321 tcgccaaccg atttcatctg agtggtcaga cggtcgttag caccggcgaa tttttcgaag
    10381 ttgaagcgag gaattttagt aaccacatag tcgatggacg gctcgaagga ggccggagta
    10441 cgtccgccag tgatgtcgtt catcagttcg tcgagggtgt aacccaccgc cagtttcgcc
    10501 gccactttag caatcgggaa accggtcgct ttcgacgcca gcgccgaaga acgggacacg
    10561 cgtgggttca tttcgataac aatcaggcga ccgtttttcg ggttcaccgc aaactggaca
    10621 ttggaaccac cggtttcaac gccgatttca cgcagcaccg ccatcgaggc gttacgcatg
    10681 atttgatatt ctttgtcggt cagcgtttgg gctggcgcga cagtgatgga gtcaccggta
    10741 tggatgccca tcgcatcaaa gttttcgata gagcagacga tgatgcagtt gtcgttttta
    10801 tcacgcacca cttccatctc gtactctttc cagccgatca gcgactcatc aatcagcagc
    10861 tctttggttg gggagagatc cagaccgcgg gcgcaaattt cttcaaactc ttcgcggtta
    10921 taagcgatac cgccgccgct accgcccatg gtaaaggatg ggcgaataat gcacgggaag
    10981 cccacgtcag cggcaaccgc cagcgcttct tccatcgtgt gtgcgatacc ggaacgcgcg
    11041 gtttccagac caattttctt catcgctacg tcgaaacgac ggcggtcttc tgctttatca
    11101 atcgcatcgg cggtcgcacc aatcatagtc acgccgaact cttccaacac gccctgacgc
    11161 tccagctcca gcgcgcagtt cagcgccgtc tgaccgccca tcgttggcag caccgcgtcc
    11221 gggcgctctt tttcaataat cttgcgtact acttcccagt gaatcggctc gatgtaggtc
    11281 gcatcggcca tttccgggtc ggtcatgatg gtcgccgggt tggagttcac cagaatgacg
    11341 cggtaaccct cttcgcgcag ggctttacat gcttgcgcgc cagagtagtc aaactcacac
    11401 gcctgaccga taacaatcgg gcccgcaccc agaatcagga tactttttat atctgtacgt
    11461 tttggcatgg ctcttttact cctgattact tagcggtttt acggtactgc tcaattaact
    11521 cgataaagtg gtcgaacaac ggcgctgcgt cgtgtggccc agggctggct tccgggtgac
    11581 cctggaagct gaacgccggt ttatcagtgc gatgaatgcc ctgtaacgta ccgtcgaaca
    11641 gggatttatg cgtgacacgc aggtttgcag gtaatgttgc ttcgtccacc gcaaaaccgt
    11701 ggttctgggc ggtgatcatt accacgtttt tctccacatc tttaaccgga tggttgccgc
    11761 cgtggtgacc aaatttcatt ttgacagtct tcgcaccgct cgccagcgcc agcagctgat
    11821 gaccgagaca gatgccgaat accggaatat cggtttcgag gaatttctgg atggcggtaa
    11881 tggcgtaatc gcacggggcc gggtcgccag gaccgttgga gaggaagatg ccgtctggat
    11941 tcattttcag cacatcttcc gcagaagttt gcgccggaac gatggtcagg cgacagcctc
    12001 tgtccaccag catccgcagg atgttgcgct tggcaccaaa atcataagcc acgacgtgga
    12061 acggcagctc gtcttctttt ttcgcttctg gcaggccacc ggtcaacgtc cagctccctt
    12121 gtgtccagct ataggcttct gcggtggtca cttcttttgc cagatccatg ccattcagac
    12181 ctgggaacgc gcgggctttt tctaaagcca gcgccgcatc cgggttatcg cccgcgataa
    12241 tgcagccatt ctgtgcgcct ttctcgcgca gtaaacgcgt cagcttacgg gtatcgatat
    12301 cggcaatcgc cacgatgtta tggcgtttca ggtaagaaga gaggtcttcg gtattacgga
    12361 agttgctggc aatcagcggc aggtcgcgaa tcaccagacc ttgtgcatgt acctgagaag
    12421 attcttcatc ggcgtcattg gtgccgacat tgccaatatg gggataagta agagtaacga
    12481 tttgacgaga ataggaagga tcagtgagga tttcttgata accggtcatt gaagtattga
    12541 aaacgacttc cccaaccgcc gaacctgttg cccctatggc ccgaccgtga aactgggttc
    12601 cgtcttccag aaccaatagc gctgacttaa tcaaaacacc ctccagagaa tattcactca
    12661 ctttatttgc atattaattc acaatgatga cgtaaatcaa tgcaaatctg cttaccgacg
    12721 aatttctggc aaacggcggc attctggaga tatgggcgct aaaagtcaac ttaatggcgg
    12781 gattttttat attattttgt ggctcatgca tgttttacag gcattaagcg acaaaaagat
    12841 catctaacct gttctggaaa acgcttgcgc ag
//
LOCUS       DAZFZC010000124        12779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFZC010000124 DAZFZC010000000
VERSION     DAZFZC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.125"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..192)
                     /locus_tag="ACHKXP_004267"
     CDS             complement(<1..192)
                     /locus_tag="ACHKXP_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7649154.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLL"
     gene            complement(339..1622)
                     /locus_tag="ACHKXP_004268"
     CDS             complement(339..1622)
                     /locus_tag="ACHKXP_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO7649155.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKG"
     gene            complement(1735..3669)
                     /gene="yeaG"
                     /locus_tag="ACHKXP_004269"
     CDS             complement(1735..3669)
                     /gene="yeaG"
                     /locus_tag="ACHKXP_004269"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO7649156.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4104..4850
                     /gene="mipA"
                     /locus_tag="ACHKXP_004270"
     CDS             4104..4850
                     /gene="mipA"
                     /locus_tag="ACHKXP_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO7649157.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4940..5794
                     /gene="yeaE"
                     /locus_tag="ACHKXP_004271"
     CDS             4940..5794
                     /gene="yeaE"
                     /locus_tag="ACHKXP_004271"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO7649158.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5845..6729)
                     /locus_tag="ACHKXP_004272"
     CDS             complement(5845..6729)
                     /locus_tag="ACHKXP_004272"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO7649159.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGIAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDANGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASEMQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6812..7807)
                     /gene="gapA"
                     /locus_tag="ACHKXP_004273"
     CDS             complement(6812..7807)
                     /gene="gapA"
                     /locus_tag="ACHKXP_004273"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7649160.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            8149..8562
                     /gene="msrB"
                     /locus_tag="ACHKXP_004274"
     CDS             8149..8562
                     /gene="msrB"
                     /locus_tag="ACHKXP_004274"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO7649161.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8604..8876
                     /locus_tag="ACHKXP_004275"
     CDS             8604..8876
                     /locus_tag="ACHKXP_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO7649162.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9246..10322
                     /locus_tag="ACHKXP_004276"
     CDS             9246..10322
                     /locus_tag="ACHKXP_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7649163.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10349..11728
                     /locus_tag="ACHKXP_004277"
     CDS             10349..11728
                     /locus_tag="ACHKXP_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7649164.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLRRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11745..>12779
                     /locus_tag="ACHKXP_004278"
     CDS             11745..>12779
                     /locus_tag="ACHKXP_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO7649165.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYQDVQQAFEESVNN
                     KRDIIKGVIKI"
BASE COUNT         3217 a         2972 c         3216 g         3374 t
ORIGIN      
        1 caataaactg aaaataaaaa agctttgagc ttttaatttt tgtgtaccca tagaaaccat
       61 aaaaaggaga gcaatgtgag tgctggattt tattgatggc agagatatat agttttgacc
      121 aggatcaatt ttcgttaacg ataaaaaata tttaaagaat aaccccatgg aaatacgtgt
      181 tgactgaatc atttttccga atgattgttt tctggaacta aattatgtaa aggttatgct
      241 gtcatattta aagcgattgt aagctaatgt atgtaataaa tgagataatt tacagtaagc
      301 taaaaatcag ccaggcgata atgacctggc tgatagtttt agcctttagc tgttgcattt
      361 tgtttatgaa acagttcacg gaacaccgga taaatatcat cctggtcgcg gatgtgctgc
      421 atcgcaaagt tgtcgaaagt agattgcaga tgctcatatt ctcgccacaa tgtctgatgt
      481 gcacgacggg taatttcgat atagctgtaa taacgaacaa caggtaataa ttttttcgcc
      541 aggatttcat ggcaaagcgg agagtcatcg gcccagttat cgccgtccga tgcttgtgca
      601 gcgtaaatat tccactgtgc cgggttataa cgctctttca ctacctcatc catcagtttc
      661 agggcgctgg aaacaatggt gccgcctgtt tcctgcgagt agaaaaactc atgttcatcg
      721 acttctttcg cctgggtatg atggcggatg tatacgactt ccacgttctt atacgttctg
      781 ctgaggaaca gatacagcag aatataaaaa cgcttagcca tatctttagt ggattgatcc
      841 attgaaccgg aaacgtccat caggcaaaac atcactgcct ggctggaggg atcgggccgc
      901 ttctcgtagt tcttgtaacg taaatcgaag gtgtcaataa aagggacgcg ttcaattttg
      961 gcacgtaatt ctgcaatttc tttgcgcaga cgttcctctt ccagcagttg cgcaggttca
     1021 ctgttgctga tgatggccaa attctcttcc agtgcatgaa gttcccgccg cttgcctgcc
     1081 gtcatggctg tgcgtcgcgc cagtgagttc tgcaatgaac gcacaacgct gatattggcc
     1141 ggaacgccgt tagcggtata acccgcccga tgcgttttat attcggtcag ctggcgttgt
     1201 tggttttgtt tcagattcgg taaggccaaa tcttcaaaga gcagatcaag atactcatct
     1261 ttcgaaatct gaaagacaaa ttcatcctga ccttcaccat cctggctggc ctggccctga
     1321 ccactgccgg aacctccgcc gccaccctgg ggacgttcaa ttcggtcgtt ctggacgaaa
     1381 tggtcattgc ccggatgcac gcggtggcgc agaccgccac gcccctgatg aaacatcggt
     1441 tcgctaatat cttccgtggg aatggatacg gattcgccgc tgtcgacgtc agtcaccgaa
     1501 cgcttattaa tggcctcgga gatcgactgt ttaatttgcg ctttataacg gcgtaaaaaa
     1561 cgctggcgat tcaccatgct tttgtttttg ccgttcagac gccggtcaat aaaccaggtc
     1621 atatgccccc cgtactacat ttgccaactg tatgctacgc ctgtgttgcc gcttctcagg
     1681 cccacggttc gctgtcgttg taggcccggt aacatgtgta ccgggccagg gttgttaaga
     1741 cgatttacgt acgcgcaaat accattcgca cagtaaacgc acctgtttac gggtgtagcc
     1801 tttttccatc atacggtcga caaagtcgtc gtgtttcttc tgctcgtcgg ttgacgtttt
     1861 ggcgttaaac gagataaccg gcaacagctc ctcggtattg gagaacattt tcttctcgat
     1921 gaccgtgcgc agtttttcat agctggtcca gttcggattg cgtccgctgt tattcgctct
     1981 ggcgcgcagt acgaagttaa caatctcgtt gcggaaatct tttggattac tgatccccgc
     2041 cggtttctcg attttctcca gctcggcgtt aagagactcg cggtcaaaca gctgcccggt
     2101 atccggatcg cgatactcct gatcctgaat ccagaaatcc gcgtaggtaa cataacggtc
     2161 gaaaatgttt tgcccatatt cggaatagga ttcaaggtag gccgtctgga tctctttgcc
     2221 gataaactcg gcatatttcg ggatcagata acctttcagg aactccagat agcgttctgc
     2281 ctgctcttgt gggaactgct cgcgctcaat ctgctgttcc aggacgtaga acagatggac
     2341 cgggtttgct gccacttcta catgatcgaa gttgaacacg cgggagagga tcttaaacgc
     2401 aaaacgcgtc gacagaccgt tcatcccttc atcgacaccg gcgtagtcac gatattcctg
     2461 atacgacttg gctttgggat cggtgtcttt cagactttcg ccatcataaa cccgcatctt
     2521 tgaataaatg ctggagtttt ctggctcttt caggcgcgaa agaatggaaa aacgtgacag
     2581 tgtttcgagc gtgccagggg cgcatggggc gtgagtcaat tcactgtgat taagcaattt
     2641 ctcgtagatt ttgatctctt cggaaatgcg caagcaatac ggcaccttca cgatgtaaac
     2701 acgatcgagg aaggcttcgt tgtttttgtt attacggaaa gtgacccatt cggactcgtt
     2761 cgagtgtgcg agaataatcc cgttgaacgg cagggcggag ataccttccg tcccgttgta
     2821 gttaccttcc tgagtggcgg ttaacaaggg atgcagcact ttaatcggtg ctttaaacat
     2881 ctcaacgaat tccatgatcc cctgattggc gcggcacagc gcaccggaat agccgtaggc
     2941 gtccgggtca ttctgcgcgt agtgttcgag tttacgaata tcgactttcc caaccagcgc
     3001 ggagatgtcc tggttgttct catcgccggg ttccgttttg gcgatagcaa tttgttgcag
     3061 aattgacggc cagaccttca ctacccggaa cttagtgata tcgccaccaa attcatgcag
     3121 gcgttttgcc gcccacggcg acatgatggt gccgagataa cggcgaggaa tgccatactc
     3181 tttttccaga atctgcgcat cttcctgcgg attgaaaaga cagaacggat gatcgttgac
     3241 cgggctacgc tcaccgttcg cgctcaatac ataaatcggt acgagctgca ttaatgattt
     3301 cagtcgctca gcaagcgatg atttaccccc acccacaggc cccagcagat acaggatttg
     3361 tttcttctct tccagcccct gagccgcgtg tttcagataa gagacaatct gttcaatcgc
     3421 gtcttccatg ccgtaaaact cttcaaacgc cggataacgt gcaatgaccc ggttagaaaa
     3481 gagtcgagaa agtctgggtt cctgggctgt atcgaccatg acaggctcac cgatagccat
     3541 caatagccgc tcagccgcgt tggcataagc actgcgatct tgccgacaag tggtaagaaa
     3601 ctcctgcagt gtgaactctt cgtccttggc agcttcatat cgctggcgat agtgatcgaa
     3661 tatattcatg gtatgccgtc ctttcgtttt ttagcacagg ttaagagccg ttcatatgaa
     3721 tagtagaggc tcccggaaga ggtaaactga acgacgtctc ttgctaaaac acagcaactc
     3781 tcatgccatt ttatcgacga tgagaacaac gtgcccggtg atacaccttc aaaattaagc
     3841 gtagatggca aatggaaaac ttgcctgcac ggctttctta atttcaatgg catatcaaca
     3901 actcatccat aaattttgca taattaatgt aaagaccagg ctcgccagta acgctaaatt
     3961 catttggctg taagcgcggt gtcatccgcg tcaggaaaat taaacagtta ctttaaaaat
     4021 gaaaacgtaa aaaggttggg tttcgatgta ttgacgggta aactttgtcg cccgctaaac
     4081 atttgtttaa ggaatgatta attgtgacca aactcaaact tctggcactt ggagtgctta
     4141 tcgcaacgtc tgcaggcgta gcgcacgctg aaggtaaatt ttccctgggc gcaggcgtag
     4201 gtgtcgttga acacccatat aaagattacg ataccgatgt ttacccagta ccggtaatca
     4261 actatgaagg cgataacttc tggttccgtg gcttaggtgg tggttactac ctgtggaatg
     4321 acgcaacgga taaactttca attaccgctt actggtcgcc gctttacttc aaagcgaaag
     4381 acagtggcga tcaccaaatg cgtcacctgg atgaccgtaa gagcaccatg atggctggtc
     4441 tgtcttatgc tcactttacc cagtacggtt acctgcgtac caccctggct ggcgataccc
     4501 tggataacag caacggcatc gtctgggata tggcctggtt gtatcgttac accaacggtg
     4561 gcctgaccgt gactccgggt attggtgtgc agtggaacag cgaaaaccag aacgaatact
     4621 attatggcgt atcgcgcaaa gagtccgctc gcagcggtct gcgtggctat aacccgaacg
     4681 acagctggag cccttacctg gagctgagcg ccagctacaa cttcctcggc gactggagtg
     4741 tttacggtac cgcacgctac acccgtctgt ctgatgaagt tactgacagc ccgatggtgg
     4801 ataaatcctg gactggcctg atttctaccg ggatcaccta caaattctga taatgcatct
     4861 ttccagggca ttttgtgcat aaaaacaggg cgtcacgccc tgttttgcat tacggtggtg
     4921 caatacctgg gaggaaacaa tgcaacaaaa aatgattcaa tttagtggcg atgtctcact
     4981 gccagccata gggcagggaa catggtatat gggcgaagat gccagtaagt gtaaaacaga
     5041 agttgctgca ctacgcgcgg gcattgagct cggtttaacc ctcattgata ccgccgaaat
     5101 gtatgccgat ggtggtgccg aaaaggtggt tggggaagca ttaaccggtc tgcgtgataa
     5161 cgtctttctc gtctctaaag tctatccgtg gaatgctggc gggcaaaaag cgataaatgc
     5221 atgcgaagcc agtttacgcc gtctcaatac tgattatctc gatctctact tattacactg
     5281 gtctggcagt ttcgcttttg aagagactgt cgcagcgatg gaaaaattga tcgcccaggg
     5341 aaaaatccgc cgctggggcg tttcgaacct tgattatgct gatatgcagg aactctggca
     5401 gctgccgggg ggaaatcagt gtgccactaa tcaggtgctt taccatctcg gttcacgagg
     5461 gattgagtac gatctactcc cctggtgcca gcaacagcag atgccggtga tggcttacag
     5521 tccgttagcc caggccgggc ggttgcgcaa tggactgtta aaaaacgcgg tagtcaacga
     5581 aattgcacat gctcacaata tcagcgcggc gcaagtattg ttggcgtggg taatcagcca
     5641 tcagggtgtg atggcgattc caaaagcggc cacgattgcc catgtccaac agaatgcggc
     5701 tgtgcttgag gtcgaacttt cgtcagcgga attagccatg ctggacaaag cgtatccggc
     5761 accaaaagga aaaactgcgc tggatatggt gtgatgttga tacgtgccgg atgcatcgca
     5821 cccggcacac aacattgacg caaattaacg tttggcaacg cgaatggatt gcgccagatg
     5881 tgcaggtttt tctttggtca ctttttgcat ttctgaggcg taagctgttt ctacacaaac
     5941 aaaggtcttg tagccatcgt ccggcatgtc gcccatgcta actgaaagcg ccggtcccgg
     6001 gttccagcct acaacgttca gatgatgctg gtggcctacg gcgataatac gattcagcgc
     6061 ttcatcatta atcacgctgc aatcttgcgg attcagatac acgcgatcgg tacggtcagg
     6121 gaaggtctga ataccgtcgg tcagtacatc ttctttcgcg tcatttactt tatcaatgaa
     6181 gcgatcgccc agcccactga cgcttacctt agcgatatca cccacgttaa agtaggtatg
     6241 cagggcagag gtggtttcaa attcgccgtg tgcttcaaga tcgatttcac aggttttacc
     6301 cacgcggaaa tgcgctaaca gagtaaagtc gtgcggccag aattttttcg tctcttcgct
     6361 ttgtgtcaat tcaaaagtca gcgctacgcc attagcatct tcacgatgtg atttcagcgt
     6421 ccacggcagg ttgcgggcaa aaccgtgcgc aggcaggcct tgttgtgccg ccggaccaaa
     6481 ccacggccag caaaccggta cgccaccgcg aatagcgatg ccatttttga acggtgtgtt
     6541 gttgctcaac cacagaactt cttcttcacc cgcaggtttc cacgagagaa ggtgtgcgcc
     6601 ctgtaatgca aaagaggctt ttacctgggg atgatcgacc acaatgaggt ccagttcatc
     6661 cagtttacga cgggagagga caggggagat ttgttcgatg accggaaggg caaaaatttt
     6721 cttaatcatg acgcagtcct ttaacttcat tttatcaggt aaaaaaagag cgaccgaagt
     6781 cgctcttttt agatcacagt gtcatctcaa cttatttgga gatgtgagcg atcaggtcca
     6841 gaactttgtt ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt
     6901 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
     6961 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
     7021 cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
     7081 ggtcaactac agatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
     7141 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
     7201 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
     7261 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
     7321 tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt
     7381 cctggccagc atatttgtca aagttagcgc ctttaacgaa catcggagtg ttgtctttag
     7441 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
     7501 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac accaacttcg tcccatttca
     7561 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
     7621 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
     7681 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
     7741 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     7801 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     7861 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct
     7921 aagcattacg cgactgactc gcctcactct tcctttgggc ttgaaaccac atggggtcgg
     7981 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     8041 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     8101 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagcaaat ggctaataaa
     8161 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     8221 catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat
     8281 cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga ttccggctgt
     8341 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     8401 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
     8461 gtcttccctg acgggccgca gccaacgggc gaacgttatt gtgttaactc tgcctcttta
     8521 cgctttaccg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
     8581 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
     8641 aggtatacca gcgtttgtcg accgccgttg aactggggaa atggcccgat ggcgttgcgt
     8701 tgaccgagga acaaaaagag aactgcctgc aactggtgat gctgtggcaa gcccgccata
     8761 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
     8821 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
     8881 tttatcccct ttttgccgcg gttataaagg ggatagccaa caatgacccc acgactcatc
     8941 tcaattatct cgccatgacc tggctaagtc ccagtccgat ctcttgccgt cgcgttcacg
     9001 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcgaa
     9061 tttgctcttt ttgcgttccg ccttccacca acctgtaaca gacccagcca ttttctgcgt
     9121 aattgtgatc taagttgtaa aacgcagatc attatctgtg ttttgcagat ttaatgttga
     9181 cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac acagaggatg
     9241 acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag atgattgatg
     9301 tcccacaacc catttgtggc ccggaagacg tagtgattga aattaaagcc gcggcaatct
     9361 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
     9421 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
     9481 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggtgtttgc ccggcctgtg
     9541 aacaaggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
     9601 ggggcggtgg tttttccaaa tattgtcttg ttcctggtga aattctcaaa attcatcgtc
     9661 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
     9721 tctgtaatgc ctacaaatcc atcgcgcagc aatcgaaatt ccttcctggt caggatgtgg
     9781 tcgtcatcgg cactggccca ctcgggctgt tctccgtaca aatggcgcga attatggggg
     9841 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
     9901 aagaactggg tgcgacggca gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc
     9961 agcaaatttg tggcaaagac aatctgggac tggtgattga atgctccggt gccaatatcg
    10021 cattgaaaca agccatcgaa atgctccgcc ctaatgggga agtagtacgc gttggaatgg
    10081 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaacaaa agcatcattg
    10141 ggcatatggc ctatgactcc acctcatggc gtaacgctat caggctatta gccagcggcg
    10201 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
    10261 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
    10321 aaccactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc attgtggtgc
    10381 acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt
    10441 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
    10501 gttgggctgg acagataatt ccaccacagc cacattctca gcaatcacga ccgccggaat
    10561 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
    10621 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caccgaatat
    10681 ggatttcctt attgcctgcc gttttgtgat gggagttggg ctgggagctt tactggttac
    10741 actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg
    10801 ggtttccttt attggcaact ggtcatatcc gctctgttca ctgattgcga tgggactcac
    10861 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttatccctg caatattgtc
    10921 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
    10981 gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
    11041 ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag cgccacaagc
    11101 ggtgccgtat tcagccttac tgacaggagt attactgaga cgcgtgatat taggctcttg
    11161 tgtgctgatt gccatgaacg ttgtgcagta cacactaatt aactggttgc caacaatatt
    11221 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
    11281 cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
    11341 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac tgcaaaccag
    11401 tatgttgctc ataacgttga ttggtttctt cctgattact ttcgtctata tgtacgtttg
    11461 ctatgcctcg gcagtgtatg tccctgaaat ctggccgaca gaggccaaac tccgtggctc
    11521 cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt
    11581 gctgctcagt agttatggcg taacgggagt ctttattctt ctgggggctg tttcaattgt
    11641 tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctctg ttgaaagttt
    11701 aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa aattcaaaag
    11761 caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacct gtgcctaaag
    11821 aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat gtacatggtt
    11881 ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc ctgggtcatg
    11941 aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa ccgggggatc
    12001 gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat
    12061 ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc ggcgcattaa
    12121 cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat atggacacga
    12181 tggaaggggc gctggtggag cctgccgcag tcgggatgca tgccgcgatg ctggcagatg
    12241 ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg atgacgttgc
    12301 aagcgtgcaa atgcctggga gcaacggaaa ttgccgtcgt tgatgtgctg gaaaaacgtc
    12361 tggcaatggc ggaacagctt ggtgcgacag tggttattaa cggcgcaaaa gaagacacta
    12421 ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc gaaacagcgg
    12481 gttctgcagt caccgttaaa caggcacctt atctggtaat gcgcggcggt aaaattatga
    12541 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaataaat cgcgaagtca
    12601 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    12661 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcag gatgtacaac
    12721 aggcatttga agagtcggtt aacaacaaac gcgacattat taaaggcgtt attaaaatt
//
LOCUS       DAZFZC010000125        12387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFZC010000125 DAZFZC010000000
VERSION     DAZFZC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.126"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..588
                     /locus_tag="ACHKXP_004279"
     CDS             <1..588
                     /locus_tag="ACHKXP_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7649166.1"
                     /translation="GKKVRTTISRKAVVAGDRVNRQFVAERPDQLWVADSTYVSTWQG
                     VVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSL
                     AYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWV
                     DWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            complement(736..1061)
                     /locus_tag="ACHKXP_004280"
                     /pseudo
     CDS             complement(736..1061)
                     /locus_tag="ACHKXP_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1107..1447
                     /locus_tag="ACHKXP_004281"
                     /pseudo
     CDS             1107..1447
                     /locus_tag="ACHKXP_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2042..3229
                     /locus_tag="ACHKXP_004282"
     CDS             2042..3229
                     /locus_tag="ACHKXP_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7649167.1"
                     /translation="MLMKYISHRAIICTLTLLIIIITVLFTFLRSSDVPEYITAPVRK
                     GDIENSVLATGRIDAIERVNVGAQVSGQLKSLKVKQGDHVTKGQLIAEIDDLPQCNDL
                     RNAEAALNEVKAELQSKQALLKQAELRFKRQLRMLRENASSHEDFESAEAMLATTRAE
                     LHSLNAKLVQAQIEVDKKKLALEYTRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKL
                     ARLDVMTIKAQISEADITRISVGQKARFSIFSEPDKHYSATLRAVELAPESVMKDDSL
                     ASNTSASGSGTSNASVYYNALFDVPNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVH
                     RNEGKKQQVLVLAADGRLEPRNVKTGITNSVDIQILEGLNVGENVVLSLPDKKEPEER
                     IML"
     gene            3226..5166
                     /locus_tag="ACHKXP_004283"
     CDS             3226..5166
                     /locus_tag="ACHKXP_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFO7649168.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGVSG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPKTNRIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGTTFRDD
                     RNALQEVIIDENTRKAIFDNTGLQALGQIVFLGSVPARVVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGIKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALADGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            5170..6540
                     /locus_tag="ACHKXP_004284"
     CDS             5170..6540
                     /locus_tag="ACHKXP_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO7649169.1"
                     /translation="MKILSSLTLCLITALCSGCTNVLKSDYRAPEVNYPINWTKGDVD
                     GNTSPFDWEEFNDPNLDNWLHLVMTSNNDIAIAALRIHRAQLDAERTGITNTPALKAA
                     LSMDGKKQLNNSSGWAKSGSASLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARL
                     MLLSEASNNYWRIGFVNQQITTLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNL
                     LTQENQLTGLQREHSQLLNQQAVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMR
                     RPDISAKEWQLREALATVDIKRSEYYPTFNLTGALGTSSASLLALLHNPVGSVGANLT
                     LPFLEWRQRDIEVKIARNDYEQRVLEFKQLLYKAMSSIEDALSFRNQLLLQETRLREE
                     LELARKSEWLNEVRYRHGAVRISFWLDAQEKRRQAELRLDENRFNQLQNLAKIYLEFG
                     GASTFP"
     gene            7014..7208
                     /gene="tnpA"
                     /locus_tag="ACHKXP_004285"
                     /pseudo
     CDS             7014..7208
                     /gene="tnpA"
                     /locus_tag="ACHKXP_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
     gene            complement(7337..8278)
                     /locus_tag="ACHKXP_004286"
     CDS             complement(7337..8278)
                     /locus_tag="ACHKXP_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4504/CBY_0614 family protein"
                     /protein_id="HFO7649170.1"
                     /translation="MAIINIYSKRQRKIRGEVNDVYQYNNIPHALRVQIIKIITDSIG
                     FPSSNERYTSYRNEADKVYAYIHEILSKEYGVFSLKEFAKNDFDALVDFFLKERNTEK
                     CLDFIEICFQILVSHVAKNHYEFKDITSQSPGDAVIELNERFREHGVGYQFESEEIIR
                     IDSQLIHADVVKPTLILLSGEPLFEGANDEFLAAHEHYRHKRYKECLNDCLKSFESIM
                     KAIHDKNNWKYSPNDTASKLINSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKNAG
                     HGQGADIKEVPEELVSYMLHLTATNLLFLLKCEKNIK"
     gene            complement(8513..9902)
                     /locus_tag="ACHKXP_004287"
                     /pseudo
     CDS             complement(8513..9902)
                     /locus_tag="ACHKXP_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996655.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(10212..10361)
                     /locus_tag="ACHKXP_004288"
                     /pseudo
     CDS             complement(10212..10361)
                     /locus_tag="ACHKXP_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10539..11732)
                     /locus_tag="ACHKXP_004289"
     CDS             complement(10539..11732)
                     /locus_tag="ACHKXP_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7649171.1"
                     /translation="METTSVIILNGFLGAGKTTLLKNLLIQAHEKQLSVSVIVNDMSE
                     LDVDGVLIANTDIVDAKDNNFVSITADSISSPAGLKKMDLAINHLLDHNKPDVMLIET
                     SGSSHPLPLVKYLRRHSRVRLKAFLTLVDTVMLHEDYNDGKKLIPTLQENLRHNKRGL
                     ENLLAEQIMFCNRLLLTRNDRLPFDIISAVAKAIHPLNPSVDVLAVSWGNIELSTLLA
                     IPDYNFDRVELLISELEALVGNMDTPCNNEELIWRVIRDDRPFHPQRLWDTCHRFMGM
                     GVYRSKGFFWLPGRDDLALLWNQSAGSISLALIGYWKAGVLEHTDNNLTREERSALQR
                     HIDTASGRFGDRCCQLTIIGNATEVNDFTHALSLCLLTEEEIQWWMSGGVFPDPWPQK
                     VTRLS"
     gene            complement(12147..>12387)
                     /locus_tag="ACHKXP_004290"
     CDS             complement(12147..>12387)
                     /locus_tag="ACHKXP_004290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649172.1"
                     /translation="RKILLVNWLRHTYQETLFPGRTYPPETPLLSGLKFFGIYRTQKN
                     ADITDRLLSVPNSADPKAMYLSTERVPDILGTDVF"
BASE COUNT         3349 a         2806 c         2931 g         3301 t
ORIGIN      
        1 ggtaaaaagg tccgtacgac catcagccgg aaagccgttg tcgcaggcga ccgcgtaaac
       61 cgtcagttcg tggcagaacg tcctgaccag ttgtgggtgg ctgattctac ttacgtcagc
      121 acatggcagg gggtcgtcta tgtggcgttc atcattgatg tgtttgccgg atacatcgtg
      181 gggtggcggg tctcatcgtc tatggaaacg acatttgtgc tggatgctct ggagcaggcg
      241 ttatgggccc gtcgaccgtc cggcacagtc catcacagtg ataaaggttc tcagtatgta
      301 tcgctggcct acacacagcg gcttaaggaa gccggattac tggcatcaac aggaagtaca
      361 ggcgactcgt atgacaacgc gatggcggag agcatcaatg gcctttacaa agcggaggta
      421 atacaccgta agagctggaa aaaccgtgca gaagtggaac tggccacact cacgtgggtg
      481 gactggtata acaatcgacg attgctggaa aggctgggcc atactcctcc ggcagaagca
      541 gaaaaagctt attatgcttc catcggaaac gatgatctgg cagcctgagt tcacagataa
      601 aacactctcc aggaaacccg gggcggttca cctacagagt accgctacca cttgtgaagc
      661 tggcagattc agattgtttt caatcgacaa cgcctcatag ttaaagtcat caacgacatt
      721 gaacgtgtga agtggtcaac aaaaactggc caccgagtta gagtttttcc agtatcgatt
      781 ttccgattcg tttggggata acccaccgtt atattcgtgc ggtcttagtg cgctgtaata
      841 tccaacgata tagaccgtta tggcgtgagc ggcctcgcag aagcttcgta acccaccacc
      901 ggcatccatt cgttcttcag actcctgaag aagcgttcca ttgggctgtt atcccagcag
      961 tttccgcgcc ggctcatact ctgcctgatc tggtatcgcc acaataactg ccggaactgc
     1021 ctgctcgtat cgctgtggaa catcaccccg ccgggcttac ccgtccagat ataggtcaca
     1081 tcaccgcacc acacctgtat agcggcgtaa tccccaaaaa cgatgctggt tgtcagtaaa
     1141 caagccatga atgacagtcg ttcctgaaat cataagacct ggaggagtgt atgctccgtc
     1201 gcagtaatta ccacgatacg cttgcagaat tttttccagt tactgaagcg tgaacggata
     1261 aagaaaaagc tcttcggaac gcgggaaaac gcctccaacg atatttttga acatcgaaat
     1321 gttttataac aataagcgtc ggcatggttc ctgcgaacag atgtcaacaa tagaatataa
     1381 aaacaattat tatcaacaac ttggaaggag taagactacc cgtggcgatt caatattaga
     1441 tcattgatgg agatataaat ggctcacttt ttatcggttg ttgacgatac gtttatcaag
     1501 tatacatgaa aatttcttca taccagctat atgagacgtt aagactaata atcttactcc
     1561 caaaatgact atgttgttgc agtttggttt attgactctt gtctatagtt caccgaatgc
     1621 caagatgagg gagcgaggaa ctttactgtc tacgcctctc ttcctggtta caaagacaag
     1681 cgtaaaactc accatccatt agaactaaag gcactttcct gagcagagtt ttcatctgca
     1741 agataaaagc tattttacag ccgtaaagtc cagtgaaacg aaagagaggt tagtgaatta
     1801 attacttgat gtattaaagt ataatgggcg tagataacac cttaatacga aatcttgtca
     1861 ggctttcccg gtaattagta atactgttga aacaaagcga gagggggtta gcccaattta
     1921 cgcaaataaa taataatctt gattatgatt aattccaaat aagtatattt ttccagtaga
     1981 tgctgggcat taactaagaa aacccagaat accactttca ttgttatgga aaacatggac
     2041 aatgttaatg aaatatattt ctcaccgcgc catcatttgt accttgacac tgttgatcat
     2101 tattatcacc gttttgttca cctttctgag aagctctgat gtgccagaat atattactgc
     2161 cccggtgcgc aaaggcgata tagaaaattc tgttctggcg acaggccgta ttgatgcaat
     2221 tgaacgggta aacgtagggg cacaggtatc agggcaatta aaatcactga aagtgaaaca
     2281 gggcgaccat gtgactaaag ggcagttgat tgccgaaatt gacgacctac ctcagtgtaa
     2341 cgatttgcgt aatgccgaag ctgcactcaa cgaagtgaaa gcagaacttc aatccaagca
     2401 ggcgctcctg aaacaggctg aactgcgttt taaacgccag ctccggatgc taagagaaaa
     2461 tgccagctca cacgaagatt ttgagtctgc ggaggcaatg cttgcgacca ctcgcgctga
     2521 attacattct ctgaatgcca aactggtcca ggcacagata gaagtagata agaaaaagct
     2581 tgcccttgaa tatacgcgag tcgtagcgcc aatggatgga attgttattg ccattgtcac
     2641 tcagcaaggg caaaccgtta actcaaacca gagtgcccca acaattatta aactcgctcg
     2701 tctggatgtc atgaccatta aagcacagat ctccgaggct gatataaccc gtatttctgt
     2761 ggggcaaaaa gcccgcttct ctatcttctc cgaaccggac aagcactaca gcgcaacact
     2821 gcgtgccgtt gaactggcac cagaatcggt aatgaaagat gactctctcg ccagcaacac
     2881 ttcagcatca ggctccggaa cttcaaatgc gtccgtttat tacaatgctc tattcgatgt
     2941 gcctaaccca gaaaatcgac ttcgtattgc catgactgct caggttaccc tgattactga
     3001 tgaagcccaa aatacactgc tggtacccat tcaggcagtg cacagaaatg aggggaagaa
     3061 gcaacaggtc ctggtgctgg cagcagatgg caggctggaa ccccggaatg tgaaaactgg
     3121 tatcacgaac agcgtggata ttcagatcct cgaaggttta aatgtcggag aaaatgtcgt
     3181 gctgtcgctg ccggataaga aagagcccga agaaaggatt atgctgtgaa aaaacttatt
     3241 gagttaaaag gggttagccg aacttatggc aacggagatc aaacgcggac cgtcctgaaa
     3301 aatgtcgatt tgacaatcgt tgctggagaa atggtggcaa tcattggagt ctctggctca
     3361 ggcaaatcaa ccttaatgaa cataatgggt tgccttgacg tacccaatag aggtgattac
     3421 tacatcgacg gacaaaatgc cgcctgtctt tcacccgatg agctggctag agtgcgccgg
     3481 gaacacattg gctttatatt ccagcgctac catttgatac ctgatcttag cgcacttggt
     3541 aacgtggaga tcccggccat ttacgccaac agcgaacgcg acagccgtcg gcaacgtgca
     3601 acggcgttgc tgggcagact gggactggaa ggacgtgagc atcataaacc ttgcgaactt
     3661 tcaggcggtc agcagcaacg cgtcagtatt gcccgagcac tgatcaacgg tggaaagata
     3721 attttggcgg atgagccaac tggtgctctg gattcgcaat ccgggcaaga agtgttagcc
     3781 attctgaatg aacttaatcg acgtggtcat actgtagtaa tggtgaccca cgatatgaag
     3841 gtcgcccggc acgcaaagcg aatcattgag ctgtgtgatg gcgaaatcat cgctgatagt
     3901 ggaggttgcg tatctgcgac ggaaacacta ccaaaaacca acagaatccg gcaaagctat
     3961 tggaaaacac tgctcgatcg aacgcgtgaa tcgatgcaaa tggcgctaaa agcgatgaag
     4021 acgcatcgct tacgcaccac gcttaccatg atcgggattg tttttggtat cgcctccgtt
     4081 gtcactgttg tggcattggg tgaaggagcc aggcaagaaa cactggaaga gattaaaagt
     4141 ctggggacaa acgtggtcag catttatccc gggcaggatt tgttcgatga cagcattgag
     4201 agtattagaa cattggtacc tgctgacgca aatgcactgg cgaagcaagg gtttatcgat
     4261 agcgtcagcc cggaagttag cgcttcagac aacattcgtt ttctgggtaa atctgcgata
     4321 gcgtccatta acggcgttgg gcgggaacat ttccgggtaa aaggtattga gttgttgcaa
     4381 gggactactt ttagagacga tcgcaacgct ctacaggaag ttattatcga tgagaatact
     4441 cgtaaggcca ttttcgacaa cacagggtta caggctctgg ggcaaatagt ctttcttggt
     4501 tctgtaccag cacgagtggt tggcatcgct aaaagtaaca accgtagtga tgcatcaaat
     4561 cgcattactg tgtggatgcc ttacagcacg gtgatgtacc gtattgtggg taaaccagtt
     4621 ctgaccggta ttagcgtcag gctaaaagac aatgtggata acgaggctgc aatcagcgct
     4681 atctcccaac tattaacccg gcgtcacggc ataaaagact tccagcttta taactttgaa
     4741 caaataagaa agtctatcga acacacctca atgaccttca gtattttaat tcttatggtc
     4801 gcatgtatct cactaatgat cggcagtatc ggggtgatga acatcatgtt gatttctgta
     4861 acggaacgaa ctcatgaaat tggcgtacga atggcggttg gcgcgagacg cagcgacatt
     4921 atgcaacagt ttattattga ggccgtattg gtttgcctga ttggtggcgc actcggtatt
     4981 gcactgtcgt atatcacagg tgcgctcttt aacgctctgg cggacgggat attcgcagct
     5041 atatattcgt ggcaggctgc tgttgctgca tttttttgct caaccttaat cggcataatc
     5101 ttcgggtatc tcccagcccg caaggcagca aggatggacc cggttatttc actggccagc
     5161 gaataatata tgaaaatttt atcatcgttg actttatgcc ttatcaccgc actctgttca
     5221 ggttgcacaa acgtgctgaa aagtgactat cgcgctcccg aagtgaacta tcccatcaac
     5281 tggaccaagg gcgatgtgga cggaaataca tccccgtttg actgggaaga atttaacgat
     5341 cctaatctcg ataactggct tcacctggtg atgacaagta ataacgacat agctatagct
     5401 gcactgcgga tacatcgggc gcagctggat gcggaaagaa caggtatcac caatactcct
     5461 gcactaaagg cggcactgag tatggacgga aaaaaacagc tgaataactc gtccggctgg
     5521 gcaaaaagcg gttctgccag ccttggcacc agctatgagc tggatctctg gggaaaaatt
     5581 gcccgtcagc gtgatgttgc tgagtgggca gtccatgcca gtgaagaaga ttttcgctcc
     5641 gcgcgcctga tgctgctttc agaggccagc aataattact ggcgtatcgg ttttgtaaat
     5701 caacaaatta ccaccctcca gcagagtatt gactatgcaa aagagacgct gcgtctggcc
     5761 gaggttcgct atcgtgcagg aaatatctcg tcgctggatg tgattgacgc gcagcaaaac
     5821 ctgctcacgc aagagaacca gcttacaggg ctacaacgag aacattcaca gctactcaat
     5881 cagcaggccg ttttgctcgg caccgtgccc ggttgccaga tagtcgagcc cacaacgctg
     5941 ccaaaaggaa gcctgcctaa ggtcaatgcg aatattcctg ccagcatact gatgcgccgg
     6001 cctgacataa gtgctaaaga gtggcagttg cgtgaggcgc tagctacggt tgatatcaag
     6061 cgtagcgaat attaccccac cttcaacctg acaggcgctt tgggtaccag cagcgcttcg
     6121 ctactggcat tactgcataa tccggtcggt tccgtaggcg ctaacctgac gctaccgttc
     6181 ctggagtggc gacagcggga catagaggta aaaattgccc gtaacgatta cgaacagcga
     6241 gtgctggagt ttaaacagct actttataag gcaatgagca gtatagagga cgcgctttca
     6301 tttcgtaacc agttgctgct tcaggagaca agactcaggg aagaactgga acttgcacgt
     6361 aagtcagagt ggttaaacga agttcgttac cggcatggtg cagtacgtat ttcattctgg
     6421 cttgatgctc aggaaaaacg tcgtcaggcg gagctgcgac tggatgaaaa ccgcttcaat
     6481 cagttgcaga atctagcaaa aatttatctt gagtttggcg gggcatcaac ctttccttaa
     6541 gtctaattgg ggacaaccag gagcaataag gagtgaaaaa tagccacagc tgaaaacacc
     6601 atagccattg acatttgagc tttacctgct cttgaaaaca aaaaatcact gttaagcgca
     6661 atgctattca cttcagcaga gcagtattgc gtgtcacgag catgtttacc attctgtgta
     6721 aatatctttt cttaaaagtg atcatcaaag cagtcatcaa actcgataaa tatccaccgg
     6781 catagcgtaa tgccgagcaa ttaaggatca attgaccgct ccttaaactg cggcactata
     6841 acggcttcta caacagggag ccgttttctt gtgcatagaa aacccccagc taggctgggg
     6901 gttccggaaa gctttcagct ttgagccagt tattaaaacc ccttttgatt tgttaaaaca
     6961 ccttgcggtc tggcaactgc aagtgtcaaa caagaaatca aaagggggtc ccaatgggga
     7021 acgaaaagag cttagcgcac acccgatgga actgtaaata tcacatagta tttgcgccaa
     7081 aataccgaag acaggtgttc tacagagaga agcgtagagc aataggctgt attttgagaa
     7141 agctgtgtga gtggaaaagt gtacggattc tggaagctga atgctgtgca gatcatatcc
     7201 atatgctaca atgtttgtca aagctcagtg aaaatgtcta atcactggct cattataaat
     7261 gtccatatat tgaaaaggcc gctgcgaaaa tccctcccgc agcggcctct ttagcataac
     7321 gtcattatgc gaaccatcac ttaatgttct tctcgcattt taagagaaat agtaaatttg
     7381 tagcagtaag atgaagcata tagcttacca attcttccgg aacctcttta atatcagctc
     7441 cttgcccatg acctgcattt ttgtttctga tggttggaat tcctgtttcc agcattgttt
     7501 tcagacttgt gaactggctt tgtaaatatg ccggaattaa attttgactt aaacagctat
     7561 ttattagttt tgatgctgta tcattaggac tatatttcca gttattctta tcatgtattg
     7621 ctttcattat actttcaaat gatttcagac aatcatttag acattcttta taacgcttat
     7681 gcctgtaatg ctcatgcgca gcaaggaatt catcattggc gccttcaaac aagggttctc
     7741 cacttaaaag aatcagggtt ggctttacga catcagcatg aatcaattga gagtctatcc
     7801 ttattatctc ttcagattca aactgataac caaccccatg ttctctaaac ctttcattta
     7861 gttcaataac tgcatctcca ggactttgac ttgttatgtc tttgaattca taatggtttt
     7921 tcgcaacgtg gctcactagt atctggaagc atatttctat gaaatctagg catttctctg
     7981 tatttctctc tttaaggaaa aagtcgacaa gagcgtcaaa gtcattcttt gcaaattcct
     8041 tcaaagaaaa aacaccatat tccttagaaa gtatctcatg gatatatgca tatactttgt
     8101 cagcttcatt tctatacgat gtgtagcgtt cattagaaga ggggaagccg atagaatcag
     8161 ttattatttt aattatttgc accctgagtg catgtggaat attgttatat tgatatacgt
     8221 catttacttc gccacgaatt tttctttgtc tcttagaata aatattaata attgccatga
     8281 aatagcctca agattttttt acatgctgtt ctgtgatact tgctgtttgg ctctttgcac
     8341 cgcgaagcgg ttcggggttc tgtgacaccc gaattttacc tcggtttcct ttgtatataa
     8401 tatcattgaa gttatttgca tttctcagaa atcttctctg gctttgattg caatagccga
     8461 aagatagcgc acacatcgcc gttggtcagt tagtacgagg tttccgccaa gttcaccttt
     8521 taccaaagat ggcctcagag cttccctgaa gctttatcat ggcttcaagt aggtcatctt
     8581 cgttcagtga gcgctgagat ttgttgatgc taggggcgtg ccgtctgcgg gaaggctcat
     8641 caccagaaca aggtaacgat tgtgagcgat tattgtcccg ttttctctgg acctgacggg
     8701 caacatccaa cacatggcca agtctcttgt tatctacaat ggcgacggga tcaacctcag
     8761 aaagccggtc ataggtggag tagggcagca gcacgccatt tagtcgcagc tccttatgtc
     8821 cgtcaggata atgccagaca tcaatatact tgccgatggc tcttcgactg tactcgttgt
     8881 cctcaatcag atagagcatt ttatcgtatt gtacagtcag attttttgat acccggcgag
     8941 gctctcgcta ggtaaattct gcatcaaggt catcgtctaa tgcctgcggc cggtgcgcgt
     9001 taaaatcatg caagggttct ttggcaaagc gccggttata atccttctgc aaatgcattg
     9061 gccgcttcta ttgtacagat ccccttaagc ctgagctcct taacgagtcg gtcctgaagt
     9121 gtcaaatgag cacgttcaac gcggcctttt gcctggctgg tttcagcaca caaaggagta
     9181 atattaaggc agcgcatggc cctggcaaac tgggtttcgc cggggccggt ggtggcgtgc
     9241 ttattattga ctctgaatac gctggcttta tcgctgtaga ggatcatcgg tttaccgtat
     9301 ttctcgatat agccacgtgt agcttcaaaa taggtaaatg tggactcaga tttcacaaac
     9361 agcaggtgca tcaggcgact ggtggcatcg tcaacataga ccagggcaga acagggtcga
     9421 ccccggtttt caaaccagtg gtggtcgcag ccatcaattt gtatcagctc gccagtacaa
     9481 ggacgccggt aacgaggctg atgaattttc ggtgcgcgtt gtctgcgggg aatccagagt
     9541 ccggccttta tcatgagacg tcggatggtt tcctttccaa ggaccagacc gtgaacttcc
     9601 tccagctttt cccgcgctag cgtggggcca aaatcacggt agtgctcccg gattatactg
     9661 agtgcctgat ctgtcagtga cgctggcaac agacggttac cagtgcggcc acgactctgg
     9721 ttgttcatgc caagcggccc atactggcga taacgtttga gcagacggct gcaatgacgt
     9781 ggcgtgatgc ccagcatttc ggcagcctgt ccaggacgga tattgcgatc gatgacatcc
     9841 tgaaggattt tgaggcgatt gatttctttc acggtgaaca ttccggcgtc atttatagcc
     9901 atagaaaact cctcatcagg aaaaagtgag tcgttgtcta gacataggga tctgagaaac
     9961 tatagcctgg acattcctaa tgagccacaa atagacatta gcattgagcc tctacaatgt
    10021 ttgtgcgcac aatctatctt atgttaaata acattgaata ttaacttact tcatgaagct
    10081 tactgcttct atttccactt ctttttctta ttaatcgctc ccctaatcca cgcattcatc
    10141 tgctctacag gctctaaaat attttttctg agtactgagc acaaactgct ccataaaaaa
    10201 cagaccacaa gtttgggcat gatactgatg tagtcacgtt ttattgtttt catgaagctc
    10261 tctgctattc cattactcac cggactccgc accgccgtgt tcttcgattt aagtcccaac
    10321 atccgagcga actggcgtgt ttcattaacc cggtagtcag agtcagcggt cgaaagacct
    10381 cccaggtagc tactctgcat acccactata atgcaactat tctcgtgacg tgtgtttcag
    10441 ctctcccccg gctatgatac gagatggtat ggagagccct tctggggcca ctgcctgcac
    10501 cagatgcaac aggaagcctg atatgaatgc aatttctgtc aggacaggcg ggtaactttc
    10561 tgtggccatg gatcagggaa tactccaccc gacatccacc actgaatttc ttcttcagtc
    10621 aacaagcaaa gggataaggc atgtgtaaaa tcattaactt cagtggcatt gccaatgata
    10681 gtcaactgac agcagcgatc gccaaatcgc ccagaggctg tgtcgatgtg tcgctgtagc
    10741 gctgaacgtt cttcacgagt taaattattg tcagtatgtt ccagtactcc agctttccag
    10801 tagccaatga gcgccagact gatgcttcct gctgactgat tccagagcag ggcaagatca
    10861 tctctcccgg ggagccaaaa aaaaccttta ctgcgataaa cgcccatgcc cataaagcgg
    10921 tgacaggtat cccataatct ttgcggatgg aatggcctgt cgtccctgat gacgcgccat
    10981 ataagttcct cattgttgca cggagtatcc atgtttccta caagtgcttc cagttcactg
    11041 atgagtaact caacacggtc aaaattgtag tccgggatcg ccagtaatgt gctcagttca
    11101 atatttcccc atgataccgc gagcacatcc acggaggggt taagtgggtg tatggctttt
    11161 gccacagcgg agataatgtc aaagggcagg cggtcatttc tggtcagcag cagcctgttg
    11221 cagaacatga tttgctcggc cagcaaattc tccaggccgc gtttgttatg ccggagattt
    11281 tcctgaagag tggggatcag ttttttccca tcattgtaat cttcatggag catgacagta
    11341 tcaacgagtg ttaaaaacgc cttcaggcgt acacgtgaat ggcgacgcag gtatttgacc
    11401 agaggaagcg gatgactgct tccggaggtt tcaatcagca tcacatccgg cttgttgtga
    11461 tcaagcaggt ggttaattgc gagatccatc tttttgaggc ctgcggggct gctgatgctg
    11521 tctgcggtga tacttacgaa attattgtct tttgcatcta caatatctgt gttggcgatc
    11581 agcacgccgt ccacatccag ttcgctcatg tcattgacta tgacagagac tgaaagctgt
    11641 ttttcgtgtg cctgtatgag caggtttttc agcagagtgg ttttccctgc gccgaggaag
    11701 ccattgagaa tgatgacaga tgttgtttcc atatcagcag tacaccagtt agttagcgag
    11761 gaaggcgctt ggtggtacaa agagcatccg gattgaatcg cataacgtcg acgggagttg
    11821 ttgtcgtttt tcctgtgagg tctcaacctg ccacagaacg caccggactt attggtattt
    11881 agtctccatc agcataatta tcaccatttt tatctccggg ggaagatttg ttctgaacag
    11941 gagtgggagg tgtgacctcc tgaaagtaat tacagattgc tagttgttgt atttttattc
    12001 gaatatgtct cttcgtatca gtgatagcac ctagataaag tatattccgg gagcgttatc
    12061 tgttctagat atcagagagt cagtcatttg taaaaaagag agctgcatca ttctattttt
    12121 cctgtctatt caccctgttg tcgtatctaa aagacatccg ttccgaggat gtcgggcacc
    12181 cgttctgtac taagatacat agcttttggg tctgcactat ttggaaccga cagaagtctg
    12241 tctgtgatat ccgcattttt ctgtgtgcga tatattccga aaaacttcaa accacttaaa
    12301 agaggtgttt ccggaggata agttcgacca gggaatagtg tttcctggta agtgtggcgg
    12361 agccagttca ccaggagtat ctttctt
//
LOCUS       DAZFZC010000126        12368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFZC010000126 DAZFZC010000000
VERSION     DAZFZC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.127"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            160..888
                     /gene="gadW"
                     /locus_tag="ACHKXP_004291"
     CDS             160..888
                     /gene="gadW"
                     /locus_tag="ACHKXP_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO7649173.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1251..4364)
                     /gene="mdtF"
                     /locus_tag="ACHKXP_004292"
     CDS             complement(1251..4364)
                     /gene="mdtF"
                     /locus_tag="ACHKXP_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO7649174.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSASYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4389..5498)
                     /gene="mdtE"
                     /locus_tag="ACHKXP_004293"
     CDS             complement(4389..5498)
                     /gene="mdtE"
                     /locus_tag="ACHKXP_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO7649175.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5606..5883
                     /locus_tag="ACHKXP_004294"
                     /pseudo
     CDS             5606..5883
                     /locus_tag="ACHKXP_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5884..6411)
                     /gene="gadE"
                     /locus_tag="ACHKXP_004295"
     CDS             complement(5884..6411)
                     /gene="gadE"
                     /locus_tag="ACHKXP_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO7649176.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7210..7782)
                     /gene="hdeD"
                     /locus_tag="ACHKXP_004296"
     CDS             complement(7210..7782)
                     /gene="hdeD"
                     /locus_tag="ACHKXP_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO7649177.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            8037..8369
                     /gene="hdeA"
                     /locus_tag="ACHKXP_004297"
     CDS             8037..8369
                     /gene="hdeA"
                     /locus_tag="ACHKXP_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO7649178.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8485..8811
                     /gene="hdeB"
                     /locus_tag="ACHKXP_004298"
     CDS             8485..8811
                     /gene="hdeB"
                     /locus_tag="ACHKXP_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO7649179.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8875..9522
                     /locus_tag="ACHKXP_004299"
     CDS             8875..9522
                     /locus_tag="ACHKXP_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO7649180.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9564..10094)
                     /locus_tag="ACHKXP_004300"
     CDS             complement(9564..10094)
                     /locus_tag="ACHKXP_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7649181.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(10250..10816)
                     /gene="slp"
                     /locus_tag="ACHKXP_004301"
     CDS             complement(10250..10816)
                     /gene="slp"
                     /locus_tag="ACHKXP_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO7649182.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(11063..12286)
                     /locus_tag="ACHKXP_004302"
     CDS             complement(11063..12286)
                     /locus_tag="ACHKXP_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649183.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPEIFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSENENRMEALAGCDFIDFNM
                     TGQDMSRLVLTLSNFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GNRIRLSNHYQNIIP"
BASE COUNT         3385 a         2701 c         2782 g         3500 t
ORIGIN      
        1 gcatttactt attatcaatt aactgttatg caaaactact ttgtggataa attttggtcc
       61 taccaaatct ggcagttttt gcgctaagaa atagtctggc atcatttcat tagtatactg
      121 aaattgaaat aatcgcagta tgaaatataa gggataatca tgactcatgt ctgctcggtg
      181 atcctcattc gtcgttcatt cgatatttat catgaacagc aaaaaatatc gctgcataac
      241 gagagtattc tgctgctgga gaaaaatttg gcagacgatt ttgcgttttg ttcaccggat
      301 acgcgacgac tggatatcga tgagctgaca gtttgccatt acttacaaaa tattcgtcag
      361 ctaccacgca atttagggtt acacagcaaa gaccgtttgt taattaacca gtcacccccc
      421 atgccgctgg tgacggcgat ttttgatagc ttcaatgaat ccggggtaaa ttcaccgata
      481 ctgagcaata tgctctacct ttcctgttta tcgatgtttt ctcataagaa agaactgatc
      541 cccttacttt tcaatagcat cagcactgtt tcaggaaaag ttgaacgcct tattagcttt
      601 gatatcgcca aacgttggta tctgcgcgat atcgcggaaa gaatgtatac cagcgagagt
      661 ctaatcaaaa aaaagttgca ggatgaaaat acctgtttca gtaaaatatt actcgcctcc
      721 aggatgtcga tggccagacg attactcgag ttacgtcaaa ttcctctgca tactattgcg
      781 gaaaaatgtg gctatagcag tacatcgtac tttataaaca catttcgaca atattatggt
      841 gtaacgccac atcagtttgc gcaacattcg ccaggtacct tttcctgaca tatttcgcat
      901 ttgaatattg gtcaggatct cacacctgct tcatgtgaaa ctcttccctg atgatttctg
      961 ccgggctacc ggctagttct ctttcgcagt ttgccaggcg gtatcggtat tttcaggagg
     1021 aaaagactcg gcatgtttgg gattattaag ctgacaattc atacgattac gggaatgtgg
     1081 gtcagtattg tgttgttcaa attgatgacg aatggctgga gtggtttcta ctttcagtgc
     1141 tgtgtactaa gcctggtatt cctgacggtt agctggttgt tgagtggtga atggttagca
     1201 ggaaagagta aggctgaacc ttcatgttca accttactct catttacacg ttacgctttt
     1261 ttaaagcggg caaagagatg ttccactaca acgaaaaaga ccggaacgaa gtaaattgcc
     1321 agcactgttg cggcaaacat cccgcccatc acgccggtac ctaccgcgtt ttgcgcgcca
     1381 gaaccggcac catgactgat aaccagcggc agcacgccga gaataaaggc cagagaggtc
     1441 atcaggattg ggcgtaaacg catccgcgcc gcttcgatga ttgcctctat cggcgttttc
     1501 ccttctttct gcatcatctc aacggcaaat tcgacaatca ggatggcgtt tttggcggaa
     1561 agcccgatgg tggtcagcaa accaacctgg aagtagacgt cattacttaa gccgcgcaga
     1621 tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac cgagaacgga
     1681 attgaccagc tctcatagag tgcggcgagg gcgaggaaca ccacgaccag tgaaatcgca
     1741 tacagcgcag gagcctgatt tgaggataac gcttcctgat acgatagtcc ggtccatgag
     1801 tagccgacgc ctgccggaag tttagcgacc aggtctgcca taaatttcat ggcgtcaccg
     1861 gtacttttcc cggccgccgc ttcacctaaa atctccattg acgggatgcc gttgtagcgt
     1921 tccagtcgcg gtgaaccata ggtccattcg gtagacgagt aggcagaaag cggtgccatc
     1981 gtgccagagg cgttgcgtac ataccattgg ttgatgttat ccggcaacat acggaacggc
     2041 gtgcctgcct ggacatacac ttttttcacc cgcccctggt tgaggaagtc gttcacgtag
     2101 ctgctgccga aggcggtgga aattgtctgg ttgatatcag acagcgccac gcccatcgct
     2161 tcagctttcg cagcgttgac gttcactttg aacatcggcg tatcttccag gccgttcggg
     2221 cgtaccccgg tgacctgatt cggtgattgc gctgccagtg ataacagctc gtttcgcgcc
     2281 tgggttagtt tttcgtgccc caggttaccg ttgtccagca gttccatatc aaaacctgac
     2341 gcggtaccca gttcagccac cgcgggtaag ttgaacggga agacgacggc tttattgata
     2401 ctgcttaacg caatcattgc ccgctgaatg atcgcggtaa ccgagttttc ctcaccgaca
     2461 cgttcagacc acggcttgag actgataaac gccaggccgt tgttttgccc ctgaccgctg
     2521 aagccaaagc cgccaacggt aaacaccgac tggacattat ctttctcttt agtcagataa
     2581 taatccgtca cctgttgcag cactttcgtg gtgttaacca tggtggcacc ggaaggtaac
     2641 tgcgcggtgg tcataaatac cccctgatcc tcttctggta agaaagaggt cggcgtgcgc
     2701 aggaacagca ccgccatccc ggcgcaaatc agcaggtaga ccaccatgta gcgaccggta
     2761 caacgcaaca gcgagcgggt gctatcggta tagtgttgag ttgatttttc aaacagcgtg
     2821 ttgaagcgtg cgaacagggc gttaggtttg tgaccgcctt ccggcgcggc tttcagaatg
     2881 gtggcgcaca gggcaggggt caggctcatt gccacaaata ctgaaagcag catggaggag
     2941 atcagcgtga tggagaactg gcggtagatc tccccggttg caccgctcat aaaggccatc
     3001 ggcataaaca ctgcggaaag aacaacggca ataccgacca gcgcacgttg gatctgcccc
     3061 atcgatttat gcgtcgcttc cttcggcggt agcttatctt ccgcaatgac acgctcgacg
     3121 ttctccacca ccacgatggc gtcatccacc agtaacccta tcgccagcac catcccgaac
     3181 atagtcaacg tgttgatggt gaaaccgacc gccgacaaga tcgcaaacgt cccgagaata
     3241 accaccggta cggcaatcgt cgggatgatt gtggcacgga aattctgcaa aaacagatac
     3301 atgaccagga agactaggat gatagcctca accagtgttt tgaaaacttc ctgaatagaa
     3361 atttcgataa acggcgtggt gtcgtaagga taaaccgtct tcagacttgc cgggaaatag
     3421 gctgataagc ggttcagttc ctctttgact gcccgcgagg tatccagggc gtttgctccg
     3481 gcagccagtt tgatggcgat cccggcagca ggtttgccgt tatagcgcgc cacggtggaa
     3541 taatcttccg ccccaagttc gacgcgagcg acatcacgca gcagcacttg cgaaccatct
     3601 tgctgaactt tcaacaggat tttgccaaat tcttccggcg tttgcagacg cgtctgcaca
     3661 atgatcgagg cgtttagctg ctggtctgcc gcctgtggca tgccacccag ttgaccaccg
     3721 gaaatctggt tgttttgcac cttaatctgg gaaataacat cggaaggtac caggttatat
     3781 ttattgagtt tttgcgggtc cagccagata cgcatggcat actcggaacc aaagagttgt
     3841 acgctaccaa cgcccgcggt acggcttagc gggtctttga tattagacgc tacatagtcc
     3901 gcgatatcgt actggttgag gctgccgtta tcagaaataa acgccgctac catcaggata
     3961 ttactgctcg acttatcgac gctaatcccc tgctgctgca ctgcttcagg taatgaaggc
     4021 atagcgagtt gcagtttatt ttgcacttga acctgtgcga tatcaggaga tgtcccagtc
     4081 tcgaaggtca gagtgataga ggcattgccc gccgcatcac tggttgaaga catgtacatc
     4141 aggccatcaa gcccattcat attttgctca atcacctgag tgaccgagtc ttctaccgtt
     4201 tgcgcatcgg cacctggata ggaagcgctg acggtaatgg tcggtggcgc aatctgcgga
     4261 tactgcgcaa ccggtaagtt catgatcgcc agaccacctg caagcatcat aataatggca
     4321 agtacccagg caaaaaccgg gcgatcaata aaatagttag ccatgaaagt ccccttaagc
     4381 ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct ggctggagga aattgctcgt
     4441 gctttgatac ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc gccagcctgc
     4501 aagccagagg tgacgaccca ctggtcgccg atggctttgc tggcttcaat ttcgcgtagc
     4561 tgcacgacat cgtctttatc cagaatgagc gccgttgctt taccctgggc gttgtgggtg
     4621 acgccttcct gcggcaccag taatacattc tggcggctac cttcatccac taatgccgtg
     4681 acgtacatgc caggcagcaa gtcaccattt gggttgggga aaatcgcccg taacgtcacg
     4741 gagcccgtgg tttcatccac tgtcgggtcg gagaatttca gcgtgccggt ctggctgtag
     4801 cgtttaccat tttccagatt gagctgtact ggcgtactgc cctgaacctg tttgatttgc
     4861 ccactggcga cctcttcttt catgcgtaag aaatcttgca ccgactgcgt gagatcgaca
     4921 taaatcgggt ccagacgttg tacggtaacc agcgaatctg cctgattagc ggtaacgagt
     4981 gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt gacattcgcg
     5041 tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac attggcttct
     5101 gcttcattca actgggtgcg cgcggtgtcg taatcctgac ggctaacgta gttggtcttc
     5161 agcaacgatg cctggcggtt aaaggtgatg cgggcattgc tggcggtaga gagcgctttc
     5221 gccagcgagc ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc aatctgatac
     5281 agcgaatcgc cctggttcac tttatcgcct tcgataaagt tgcgtttaat gataataccg
     5341 cccacctggg gacgtatctc ggcaacttca taaggaacgg ttctaccggg caattcgctc
     5401 aacacattga ccgaaccggg ggagagtgtg acgacaccga cctcaggcgt catggcggcg
     5461 gcgttttccg ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa taacaacggt
     5521 attaacagct ttcttcttct gttcatttta gtccctgaaa attcttgaga aataacggtt
     5581 ctttaaattg cgcgcgagga gagatatgag ggatttacag accagcggca tagtggggtt
     5641 gtcagccagt aaggtgggat acaggcacag tgatcgacat ggtgaggtca acgacaaaaa
     5701 aatgttgccg ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt
     5761 ggatacacat accaggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt
     5821 aatgaaatca gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc
     5881 tgactaaaaa taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag
     5941 cttgtacata atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga
     6001 cgtgatattg ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg
     6061 tgaaaaacat aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag
     6121 atttttgata ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact
     6181 gatgtgataa ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat
     6241 cgcttcttca tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt
     6301 gcctactttt ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa
     6361 ctgccaaaag ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc
     6421 cttagccgtt atcgtttcga atatgtcatc cttttcatta tttacatcct tgtccgaatc
     6481 gttgttcaat atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt
     6541 aacactaatc aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag
     6601 attcctggtt gttatcagct tgtaaatagc aaacaaaaag ttaacgtttg cggattactg
     6661 cccaagaata agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa
     6721 atgtaatccg atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc
     6781 cagctttctt ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt
     6841 tttatatagc gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa
     6901 aatgaacccg gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt
     6961 cactttccca cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata
     7021 atgtttgaaa tggctaattt gccatagagt gaaaaaaatt agatgaaatt cagtaggttg
     7081 aaataatcac tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactgcg
     7141 tagcggagag agtattaatc ggatcatagt cacatcaagt gactatgatc cgggtgacaa
     7201 ccggggtaat tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct
     7261 aaatatcagt tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc
     7321 accaaggaat atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct
     7381 gcctttcatt gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa
     7441 caaaccggca ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa
     7501 atagccgatc aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt
     7561 atgactgcgg ttgctgaata acccgacaat aagcgcaata cccgagcaga ttaataatgc
     7621 acccactact gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc
     7681 gacgataaac agcagcacgg caataaactg gattgctctg cgatgttttt taagcatctc
     7741 cagatcaaac ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat
     7801 taagaagaaa atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgcagtcgat
     7861 ttaataaaaa tttcctaatt gcagtatctg atgcatctgt aactcattgt attgaaataa
     7921 aaatatctga ttttgatatt ttccatcaac atgacatata cagaaaacca ggttataacc
     7981 tcagtgtcga aattgattcg tgacggctct ttcactttat agttgaggat attacgatga
     8041 aaaaagtatt aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag
     8101 cggatgcgca aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt
     8161 tcctggctgt ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca
     8221 acaaagataa accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag
     8281 ctatcgttca ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg
     8341 aatgggacaa aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg
     8401 catattgaac aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa
     8461 tctctatatt gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt
     8521 atgggcgctg tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa
     8581 tccgctaaag atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg
     8641 gttgcatggt ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta
     8701 aatgaaaccg atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag
     8761 aaaaatttgt ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtgca
     8821 agtaaaaagg agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca
     8881 gcagaattta ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa
     8941 aggcaaatgc gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc
     9001 gccctgttta tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc
     9061 ggactcgacc ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca
     9121 ggtaacattc tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg
     9181 gtgaccgccg ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt
     9241 tcagtgatga ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac
     9301 aaaaattacc atttacagct gacattagtg aacggtaata cggtatcaat gctcgactgg
     9361 tttaagcagc aaaaaataaa aacggattta gtttcgttgc aggaaaatga agatcatgaa
     9421 gtggtcgcca ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta
     9481 ttaaaaggta tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc
     9541 cagactcacc gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc
     9601 tcattgatcc gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt
     9661 tttagcgata ttttcagttt ctccagaata tcttccgttg tcatttgatt cgcaatgcag
     9721 ctcagaacct gatgttgctt gcgcgaaagt gacacagccg cttcaggcac agagcagtaa
     9781 ctgccattga tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc
     9841 ggcactttcc ccaggcagcg tttaatatta aagggggaga aaataatgac atggacaggt
     9901 tttaatcgtt caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca
     9961 ataatgacga aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga
    10021 acgacattac ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta
    10081 attataagaa acataaccga ctcctggtgc ggacattaac tttataatca gtttgccaaa
    10141 ggtaattaac aggacagggc ttataataat aatagttagc ttactgtgta ttaatttgat
    10201 gtaatgcatg atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca
    10261 gctcaggtgt tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg
    10321 gccagaatgc accatagccg taatcccaca ggttataggt ggtattaacc acttctctca
    10381 aatgccacac ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga
    10441 taaagcctgg ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt
    10501 tcactggatc aaggaagccg ctttgtctgg cgagcagtcg gccctgataa ttggcttcaa
    10561 tatcaggctt cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt
    10621 tgccattgat aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc
    10681 ccggctggtt atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga
    10741 tattttgcgg aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac
    10801 cttttgtcat gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt
    10861 ctttatagtt ttcatctgat tctgaggtga tatgcctgat agtaacgtaa acctgtctga
    10921 tgtttatatc ccttctgttt agttcaattc cgttattttg gggcgtggta tgagtatcca
    10981 tcgcccaata atataaccct ttgttttaaa attacataaa atctattttt attcaatgat
    11041 tataaagtgc taataatcaa tattatggta ttatattctg ataatgattt gataagcgaa
    11101 ttctgttgcc agatggtatt tctatttcca ccccataatt tatatctttt tcattaggca
    11161 gatctctctt taaataaaag gggtatgaaa tcttttcggg ttgaagtgtc tcgtaatgaa
    11221 atgctatgaa ttcattttta gacattgttg cccatttttg aattttattg tatagagagg
    11281 gtgttgtaaa ctcttgaagt tgtttataac atattttttt gaactcttgc ttatatttta
    11341 atatgtcatc aaatgaaaga ttcactacct ttgcctctaa gaaatcaatt attctcgaag
    11401 caatttccgc tttcgcgcgt aaagagttgt cattaatagt taacaatgta gaaaacaagc
    11461 cattgatttg tttgtcaaga tgctgttcat atttatgtag tttggggatg ggattttcct
    11521 tgtgtgaaaa aatagtgtta aataaatttg cgtcagtgaa atttatatta agtagatctt
    11581 caaaataaaa atttgataag gttaggacta gcctggacat atcttgccct gtcatgttga
    11641 aatcaataaa gtcgcatcca gcaagggctt ccatcctatt ttcattttca ctgtgctcaa
    11701 tatacagctt cgccattttt aagtacaagc tttgaatgtc tcttatatca gttggtaagt
    11761 cagctcgttg cccagcaaaa tttttattat cggaagaaac ggtttggaca ctgaatattt
    11821 cgggtattcc ttcttctacg agaattttag ttatgattgg attgttgggt tctggattaa
    11881 cgacaacaat gatttgacat acagaatcct tatccttttc gtatctaatg ttatgaatct
    11941 gattaagtat tgattgtgtc tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat
    12001 taataagact tacaagttga accacaactt ttgagtagtc tgcatttgta tatgtggtgt
    12061 tcgtgtggca tgcattaatt tgttggagtt gttgttgcgc taaatcaaat tgttcaggat
    12121 tgtatccttc tccagagaat gtggaattaa tgggttgttg ttcttcccaa ttatcacaag
    12181 gccacttcgg tgtgtctggc gttttaacat tatcggtatt tgttgtgcag ttataaatat
    12241 cgccgtgata tgtatttatt atacctgggg taaagtcaat tgacataaca ttccttattc
    12301 atcatctgtt ttatgctaag gtttttaaat tggataatta gaacaggaat ggattttctt
    12361 ctacacac
//
LOCUS       DAZFZC010000127        12144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFZC010000127 DAZFZC010000000
VERSION     DAZFZC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.128"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            218..856
                     /locus_tag="ACHKXP_004303"
     CDS             218..856
                     /locus_tag="ACHKXP_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7649184.1"
                     /translation="MRRLPVYLLLDTSGSMHGEPIEAVKNGVQTLLTTLKQDPYALET
                     AYVSVITFDSSARQAVPLTDLLSFQMPALTASGTTSLGEALTLTASSIAKEVQKTTAD
                     TKGDWRPLVFLMTDGSPNDDWRKGLNDFKAARTGVVVACAAGHDADTSVLKEITEIVV
                     QLDTADSSTIKAFFKWVSASISVGSQKVESSKKEVIGLEDLPPPPPEVNVVL"
     gene            879..1517
                     /locus_tag="ACHKXP_004304"
     CDS             879..1517
                     /locus_tag="ACHKXP_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7649185.1"
                     /translation="MSVSLSKGQGVSLKKNEYDLSSVTIGLGWDINEEKKGFLGGIFG
                     KKEEYDLDVIAFLCNSAGKVTDLGNVENGKPTLVNGDIIFFNSLRHKSGNIWLTGDNR
                     TGAGDGDDEQIIVRLNSLDAQYEKIVFIVQIYNGEKLQQHFGKVQNAFIRAVDARNIE
                     MARFDLSGGPAFASQRSMVFAELIREATGWKLRAIGEPSESDSFVSHLRNYM"
     gene            1517..2155
                     /locus_tag="ACHKXP_004305"
     CDS             1517..2155
                     /locus_tag="ACHKXP_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7649186.1"
                     /translation="MRRLPVYLVIDTSGSMRGESIHSVNVGIQAMLSALRQDPYALES
                     VHISIITYDNEAREFIPLTPLEDFQFSDIVVPSAGGTFTGAALECLMQCVERDVRRSE
                     GDTKGDWRPLVFLMTDGTPSDALAYGEAVKAIRGRGFGSIIACAVGPKAGHEHLKQLT
                     DKVVSLETLDSTAFAGFFKWVSASVSSGSTSAGINNGTDTLPPPPPEIQLVL"
     gene            2240..3280
                     /locus_tag="ACHKXP_004306"
     CDS             2240..3280
                     /locus_tag="ACHKXP_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerY-C metal binding domain-containing protein"
                     /protein_id="HFO7649187.1"
                     /translation="MRRLPVFFVLDCSESMIGENLKKMTDGLQMIVGDLRKDPHALET
                     AWVSVIAFAGVARTIVPLHEIASFYPPRLPVGGGTSLGAALRELTVQIDTQVRKTTHE
                     AKGDWKPVVYLLTDGRPTDDTTAEVKRWKDHYASKVNLIAVGLGPSADLNILRQLTEN
                     VMLFTESQEGDFTRFIKWITASVTAHSRSVGDDKQPELSQTEYIVRLAKDEPVKAYDE
                     NCVTLTGRCSKTRRPYLMKYERPPARVSGLDFSLNLNSFNIAGCYPIDEDYFAWSDAT
                     ATGLQVNTSELHGVPGCPHCGNASAFALCSCGKLLCIDGPDDVICPWCETGLSFSNDG
                     GNTNFDVNRGRG"
     gene            3280..4917
                     /locus_tag="ACHKXP_004307"
     CDS             3280..4917
                     /locus_tag="ACHKXP_004307"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO7649188.1"
                     /translation="MSQNALLEEKIIRLVLSELGHPVEGERLFLLSHAPSLTEEIMRL
                     KERIESLAQGLSARVSEGDNDAEIAASSASSLSDQQQTVVSDADNPGIDATDTPLLQP
                     LPENEPHPPFWKLMPYYEFDKPAQREDENLSSLVLKPGQIPTGQQRARLAVPPAVPPR
                     AKIIIPNARAGERFSSPVAIVLDEGQQATVRDVVFPRNIGLTFDKEHKLLTGTPTENG
                     DIELSVLWSCASHDECETKQLFIVNPDPRSLWKVVEPPADASYPKSHLDAAGLVRGDI
                     RIAAASRRGRSHEHAGSFRDDDFYINHCQETGWSVMLVADGAGSAVNSREGSRIAVQT
                     AGDYLFNQLSGLKGIHLKQHITAWEGSDQQATIKAMLHHFKQAATLAVNSIQNEAICA
                     EQPVKSYSTTLLATVALRTDKELFAAAFWLGDGAIGAYSPSGKVRILGNPDSGEYAGQ
                     TRFLDQSIIADPSFSSRISVGKWNDVSHLILMTDGVSDPLFETDNGLRSDEKWTRLLD
                     ELIPVLTDASIAPERLGDWLNFFSTGNHDDRTIAVLW"
     gene            4943..6442
                     /locus_tag="ACHKXP_004308"
     CDS             4943..6442
                     /locus_tag="ACHKXP_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO7649189.1"
                     /translation="MANIVTCKTKDGETVQYVDEVIGSGSMKDVYFSPDKSYVVAFYH
                     KPQNEQARDRIDMITGRYRQNIFGQSGGEYWKDLFCWPTHVVEHGDKIGIVVPTYKSY
                     FFFKYGSKNDDFLGIKGREKEGKWFASASNQNKFLDPRERGNTLTYLKVCLLLTRAVR
                     RMHAAGLCHSDLSYKNVLIDPEMGHACIIDVDGLVVPGKYPPDVVGTPDFIAPEVVKT
                     SHLSKEDPNRVLPSITTDRHALSVLIYMYLFFRHPLRGGKIHDMSDEVRDETLSMGEK
                     ALFIEHPTDKSNAVKVSQLSSFSLPWADPEKIPYTIMGPYLTPLFERAFIDGLHDATK
                     RPTADEWESALVKTVDLIQPCQNKACEQKWYVFSGKTKPVCPYCSTPYKGKLPVLNLY
                     SSRKEGSYRPDDHRLMVWSGQSIYAWHVNRLIAPNERTTDAQRKRVGYFVFHNDQWWL
                     VNEGINGLMSLPDKRQIAIGEKIELTNNAQFVLSKEEGGRLVVVQLVEN"
     gene            6838..6993
                     /locus_tag="ACHKXP_004309"
                     /pseudo
     CDS             6838..6993
                     /locus_tag="ACHKXP_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
     gene            7522..7981
                     /gene="tnpA"
                     /locus_tag="ACHKXP_004310"
                     /pseudo
     CDS             7522..7981
                     /gene="tnpA"
                     /locus_tag="ACHKXP_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8099..9037)
                     /gene="hypT"
                     /locus_tag="ACHKXP_004311"
                     /gene_synonym="qseD"
     CDS             complement(8099..9037)
                     /gene="hypT"
                     /locus_tag="ACHKXP_004311"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO7649190.1"
                     /translation="MKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKALEA
                     AIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAAAH
                     SLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQSPF
                     CCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGITG
                     RTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIYRM
                     NTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            9388..10107
                     /locus_tag="ACHKXP_004312"
     CDS             9388..10107
                     /locus_tag="ACHKXP_004312"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO7649191.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            10173..11471
                     /locus_tag="ACHKXP_004313"
     CDS             10173..11471
                     /locus_tag="ACHKXP_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HFO7649192.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIYAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
     gene            complement(11715..11870)
                     /locus_tag="ACHKXP_004314"
                     /pseudo
     CDS             complement(11715..11870)
                     /locus_tag="ACHKXP_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11956..>12144)
                     /gene="istB"
                     /locus_tag="ACHKXP_004315"
     CDS             complement(11956..>12144)
                     /gene="istB"
                     /locus_tag="ACHKXP_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HFO7649193.1"
                     /translation="LMYQRGSIIVVSQLPVENWYKMIGDSTHADAILDRLVHGSIKIE
                     LKGESMRKIQSPLTEGDQ"
BASE COUNT         3086 a         2839 c         3051 g         3168 t
ORIGIN      
        1 tcggaatctt atattccagt acggtctgaa tttttttctg ttgataagag gccattcaca
       61 tgtcggtaat tcaatggtgg ttaacttatt tctataatgc cgcttctgag tatttaatct
      121 ccccccgaca gatgtcgtgc cgttttatca aatcaacaaa ctctggcctc ttcctgaaac
      181 tactctcctc ttccgatctc ttaagaagga attacatttg agacgcttac ccgtatatct
      241 gctactcgac acgtccggct ctatgcacgg agagcctatc gaagcggtta agaatggcgt
      301 tcagacgctg cttaccacgc tgaaacagga tccgtatgca ctcgaaacgg cttacgtgtc
      361 agtgatcacc tttgattctt cagcccgaca ggctgtcccc ctgacagacc tcctgagttt
      421 ccagatgcca gcactaacag ccagcggcac cacgtctctt ggcgaagcgc ttactctcac
      481 ggccagctcc attgccaaag aagtacagaa aacgacggct gacactaaag gtgactggcg
      541 tccccttgta ttcctgatga cggatggaag tccgaatgat gactggcgta aaggcctgaa
      601 tgactttaaa gccgccagaa ccggcgttgt tgtggcatgt gcagccgggc atgatgccga
      661 taccagcgtc ctcaaagaaa tcactgaaat cgtggttcag ctcgatacag ctgacagttc
      721 gacgattaaa gctttcttta aatgggtcag tgcgagcatt tcggtaggca gtcagaaagt
      781 ggagtccagc aaaaaagaag tgatcggtct tgaagaccta ccaccgccgc cgccagaagt
      841 aaatgtggtc ttataatttt attgattaag gggatgtcat gtctgtcagt ctgagcaaag
      901 gccagggcgt aagcctgaaa aaaaatgaat acgatctctc gtccgttact atcggcctgg
      961 gttgggatat taatgaggaa aagaaaggtt ttctcggcgg gatctttggt aaaaaagaag
     1021 aatacgacct tgatgtgatc gctttcctgt gtaattcagc cggaaaggtg accgatctcg
     1081 gcaatgtgga aaatggtaaa ccaacgcttg tgaatggcga tatcatcttt ttcaacagcc
     1141 ttcgccataa gtcaggcaat atctggctga cgggcgataa ccgaaccgga gccggtgacg
     1201 gtgacgatga gcaaattatt gtgcgcctga attcccttga cgctcagtac gagaaaattg
     1261 tgttcatcgt tcagatctac aatggtgaaa agctccagca gcactttggt aaagttcaga
     1321 atgccttcat ccgggcagta gatgcccgta atattgaaat ggcacgattc gatctttctg
     1381 gcggacccgc cttcgctagc cagcgttcca tggtctttgc cgagctgata cgcgaggcta
     1441 caggctggaa actcagggca attggtgagc cttcagaatc agattcgttt gtctcgcacc
     1501 tgaggaatta catgtgatgc gccgcctgcc tgtttacctt gttatcgaca catccggctc
     1561 gatgcgcggt gagtcaatcc attccgttaa cgttggaatt caggcgatgc ttagcgctct
     1621 tcgccaggat ccctacgccc tcgaaagcgt tcatatctcc attatcacct atgacaatga
     1681 ggcgcgtgag ttcattcctc tcacgccgct ggaagacttc cagttttctg acatcgttgt
     1741 gccaagcgca ggcgggacgt tcaccggtgc tgcccttgaa tgtctgatgc agtgtgttga
     1801 acgggatgta cgtcgctcag agggtgatac caaaggagac tggcgtcctc tggtattcct
     1861 gatgactgat ggaacccctt ctgatgccct ggcgtacggt gaagcggtaa aagcgattcg
     1921 cggccgggga ttcggatcca tcattgcctg cgccgttggt cctaaagcag gccatgagca
     1981 cttaaaacag ctcaccgata aggttgtgtc tctggagacg ctagattcaa ccgcgtttgc
     2041 aggtttcttt aaatgggtat cggccagcgt gtcttccggc agcacaagcg cggggattaa
     2101 taacggaact gatacccttc cccctcctcc accagaaatc cagctggtgc tctgacagac
     2161 agaaaggtgc tgtttccggg caacaccttt ctgtacagca acgttttctg ctggccgtac
     2221 cagccgtacg aggaaaatta tgagacgcct tcccgtgttt tttgtcctgg actgttcaga
     2281 gtccatgatt ggtgaaaact tgaaaaaaat gaccgatggt ctgcaaatga tcgtcggaga
     2341 tttaagaaag gatccacacg cacttgaaac agcctgggtt tcggtaatcg catttgccgg
     2401 tgtagcccgt acgattgtac ctcttcacga aattgcctcg ttctaccctc cccgccttcc
     2461 cgttggcggc ggtacgagcc ttggggccgc attgcgtgag ctgaccgtgc aaattgatac
     2521 tcaggtcaga aaaaccactc atgaggctaa aggcgactgg aaacccgtcg tgtacctcct
     2581 caccgacgga cgtccgactg acgacacaac cgcagaagtg aagcgctgga aggatcacta
     2641 cgcgagtaaa gtgaatctca ttgccgttgg cctggggccg tcagcagacc tgaatatcct
     2701 gcggcaactg acagagaatg tcatgctgtt caccgagtct caggaagggg actttacccg
     2761 cttcatcaaa tggatcacgg cttcggttac ggcgcacagc cgcagcgtcg gggacgacaa
     2821 acaacctgag ctcagccaga ctgaatacat agtccggctg gccaaagacg agccagtaaa
     2881 agcgtacgac gaaaactgcg ttacgcttac cggccgttgc agcaagaccc gtcgtccgta
     2941 tctgatgaag tatgaacggc caccagcaag ggtttccggg ctcgatttca gcctgaacct
     3001 gaacagcttt aatattgccg gatgctatcc catcgatgag gactattttg catggtcaga
     3061 tgccaccgct accggtttgc aggtaaacac cagcgaactg catggcgtac cgggttgccc
     3121 tcactgcggt aatgccagtg cgtttgccct gtgctcatgc ggaaaattgc tgtgcattga
     3181 cggcccggat gacgtgatct gcccgtggtg tgaaacaggt ctgtcattca gcaatgatgg
     3241 cggaaacact aacttcgacg taaacagagg gagaggttga tgtcgcaaaa tgctttactt
     3301 gaagagaaga ttatccgctt agtgctgtct gagctgggcc accctgtaga aggtgagcgg
     3361 ctttttcttc tatcgcacgc cccttccctt actgaagaga ttatgcggct taaagagcgg
     3421 attgagagcc ttgcacaggg tttatctgcg cgggttagtg aaggtgataa cgacgcagag
     3481 atcgccgcct cgtcagcatc cagcctttcc gatcagcaac aaacggtcgt aagtgatgct
     3541 gataacccgg gcattgacgc taccgataca ccattactgc aacccctgcc agaaaatgaa
     3601 cctcatcctc cgttctggaa gctgatgcct tattacgaat ttgataaacc agcacagagg
     3661 gaagacgaaa acctgtcttc cctggtatta aaacccgggc agataccgac aggccagcag
     3721 cgggccagat tagctgtgcc acctgccgtt cccccgagag ctaaaataat tattcccaac
     3781 gcccgcgcag gcgaacgttt ctcttcgccg gttgctatcg ttctggatga gggccagcag
     3841 gctactgtca gggacgttgt ctttccccgg aacattggcc tgacctttga caaagagcat
     3901 aagttgctga caggtacacc caccgaaaac ggcgatatag agctttcagt actctggtca
     3961 tgcgcttcac acgacgaatg cgaaacaaaa cagcttttta tagtcaatcc cgatccgaga
     4021 agcctgtgga aggtggtaga gccaccagca gacgcttcct accctaagtc ccatctggac
     4081 gctgctggcc tggtcagggg tgacatacgt attgctgccg ccagtcgccg gggacgatct
     4141 catgagcatg ccgggagctt cagagatgac gacttctata tcaaccactg ccaggaaaca
     4201 ggatggtctg tcatgctggt cgcagacggg gccggtagtg cggtaaattc ccgggaaggc
     4261 tcccggattg cagtccaaac ggctggcgat taccttttca atcagctcag tggtttgaaa
     4321 ggcatccatt taaagcagca cattacggcg tgggaaggga gcgatcagca ggcaaccata
     4381 aaagccatgc ttcatcattt taaacaggca gctacgctcg cggtcaacag tatccagaac
     4441 gaagccattt gtgcagaaca acctgtgaaa tcctattcaa caaccctact ggcaactgta
     4501 gcgctacgaa ctgataaaga gctgtttgcg gctgcattct ggcttggtga tggtgcgata
     4561 ggtgcataca gcccttccgg taaagtcaga atactgggaa atcccgatag cggagaatac
     4621 gcaggacaaa cccgctttct tgaccaaagc attatcgcag acccctcgtt tagcagccgc
     4681 atcagcgtgg gtaaatggaa tgatgtatct cacttgatcc tcatgacaga tggcgtatca
     4741 gaccctctgt ttgagacgga taacgggctt cgcagtgacg aaaaatggac ccgtctcctt
     4801 gatgagctca tccctgtcct tacagacgcc agtattgcgc ctgagaggtt aggcgactgg
     4861 cttaactttt tctccacagg gaaccacgat gaccgcacta ttgcggtgtt gtggtaagag
     4921 atacgctttt tcggggatca atatggcaaa tattgttacg tgcaaaacaa aggacggcga
     4981 aacagtccag tatgttgacg aggtaattgg ttcgggctcg atgaaggatg tttacttctc
     5041 gcctgataaa tcatacgtcg tcgcttttta tcataaaccg cagaacgagc aggcccggga
     5101 tcggattgat atgatcaccg gacgctacag gcaaaacatt tttggtcagt ccggaggtga
     5161 atactggaag gacctgtttt gctggccgac ccacgttgtt gagcatgggg ataaaatcgg
     5221 catcgtggtt ccaacctaca aaagctactt tttctttaaa tacggctcta aaaacgatga
     5281 ttttcttggc ataaaaggcc gtgaaaagga aggcaaatgg tttgccagcg ccagcaacca
     5341 gaataaattt ctcgacccac gtgaacgagg caatacgctt acctatctca aggtctgtct
     5401 gctgctaaca agagccgtca gaaggatgca tgcggcaggt ctctgtcaca gcgatctaag
     5461 ctataaaaac gtgcttatcg atccagaaat gggacatgcc tgcatcattg acgtagacgg
     5521 cctggttgtc cctggaaagt atcctcccga cgtcgtgggt accccggatt ttatcgctcc
     5581 ggaagtggtg aaaaccagcc atctttccaa agaggatccg aaccgcgtat tgccaagcat
     5641 tactactgac cgtcatgcgc tgtcggtgct tatctacatg tatctgttct tccgtcatcc
     5701 gctacgtggc ggaaaaatac atgacatgtc ggatgaagta cgtgatgaga ccttatctat
     5761 gggtgagaag gcgctcttca ttgaacatcc gacagacaaa agcaatgcag tcaaagtcag
     5821 tcagctatcc tccttttcac tgccctgggc tgacccggag aaaattcctt acaccatcat
     5881 gggcccttat ctgactcctt tgtttgaacg cgcctttatt gatggcttac acgatgccac
     5941 taaacgcccg accgccgatg agtgggaaag tgccctggtt aaaacagttg atctgataca
     6001 gccctgccag aacaaggcgt gtgaacagaa atggtacgtt ttctcaggta agacaaagcc
     6061 ggtttgtccc tactgcagta cgccatacaa gggtaaatta ccggttctaa atttatattc
     6121 atcccgaaag gaaggcagtt atcgtcctga cgatcaccgg ttgatggtgt ggagcgggca
     6181 gtcaatctat gcgtggcatg tgaatcgcct cattgcgcca aatgagcgca caaccgatgc
     6241 acaaaggaaa cgagttgggt attttgtttt ccataacgat cagtggtggt tagtaaatga
     6301 aggcataaat gggcttatgt cattaccgga taaacgacag attgccattg gtgaaaaaat
     6361 tgaactgacg aataacgctc agtttgttct gtcaaaggag gaaggcggca ggctagtcgt
     6421 cgttcagtta gttgaaaact aaccgtaaga catgggaaaa gatagcaact gagatcctaa
     6481 gtttgaatgt tatcacgcct gcgtttgaga aacttcgttg gaaaagtaac ctcggtaaac
     6541 ccccgtgccc tgagctcatt ccggagatgc tgtcatagtg accgtcttgc tattggtggg
     6601 aattggagac tgaatgtttt gacaagacag tccgggaaat tctgggtgta atcatataag
     6661 acaggtgata ttgcgttctc caacccttta gctcagtggt aagaggactc ataatcgctt
     6721 ggtcgctggt tcaaatccag taagggccac catcacacac tgccactggc ccattgcgcc
     6781 atgcccggcg catatcagcc acagagcctt tcggggataa tttgcagagt ggtctggctg
     6841 actttctctg taggcagatc gttcccgctt gatagctcat cccccaaaga aacgtcacga
     6901 tatttggtat tttcaaaatt caaaaagaaa acccgcagag tggcaacaga gattaaaaag
     6961 tttgagaaac gcgatctggc acaggcggtg attggtgttg gagtgatggt agattttatc
     7021 agccggataa tgtcggttct ggctgatagc tggggttgtt ggtgtggtct tcctattgat
     7081 gaaaaaacaa gcaattactt ggcatgttat ctgtaacact ttaaaactgt taagaagatc
     7141 acaattcatt atgtttatta ttgtctggcc ggagggatat ttgttaaaaa gtgcgccgtg
     7201 tggtgaatcc ccctgtgcgg tggggcgact ggtgtacata gcatttatta tgttatgagc
     7261 gaagcctacg cgggttcagt gacaccgact gaattcaccg ggaggcaccc ggcaccatgc
     7321 aacagacgca agttaggcta agccttatac ccctgtaagt tttttacagg ggttttttgt
     7381 gcacagaaaa ctcccagcta ggctgggggt tccggaaagc tttcagcttt gagccagtta
     7441 ttaaaacccc ttttgatttg ttaaaacacc ttgcggtctg gcaactgcaa gtgtcaaaca
     7501 agaaatcaaa agggggtccc aatggggaac gaaaagagct tagcgcacac ccgatggaac
     7561 tgtaaatatc acatagtttt ttgcgccaaa ataccgaaga caggtgttct acagagagaa
     7621 gcgtagagca ataggcagta ttttgagaaa gctgtgtgag tggaaaagcg tacggattct
     7681 ggaagctgaa tgctgtgcag atcatatcca tatgcttgtg gagatcccgc ccaaaatgag
     7741 cgtatccggg tttatgggat atctgaaagg gaaaagcagt ctgatgcttt acgagcagtt
     7801 tggtgatttg aaattcaaat acaggaacag ggagttctgg tgcagggggg actacgtcga
     7861 tacagtgggt aagaacacgg cgaagataca ggattacata aagcaccagc ttgaagagga
     7921 taaaatggga gagcagttat cgattccctg tccgggcagc ccgtttacgg accgtaagta
     7981 acgaagttgg atgcaaatgt cagatcgtgt gcgcctgtta gggcgcggct ggtaagagag
     8041 ccttataggc gcatttgaaa aacctccggc tatgccggag gatatttatt gcacgtcgtc
     8101 aattgcggcg tgctggctct tccattgata tctgctggat taaatcgtct ggcatatgtt
     8161 cctgtaaaat acgccagagg ttctcggcgg ttctattcag tcgggtgttc attcggtaaa
     8221 tataagcctg aatggggact gtcaatttag cagcatcaag gcatatcaga cgtttggtct
     8281 gtagttcatc gacgatcgac cagatcggta accaggcaat gccgtatccg ttgagcgcca
     8341 tattctttaa taactcgctc atcgaagaga taaatatcgt gcgcccggta atacctccaa
     8401 cctctgccag atgacgattg accaacctgc ccatataaga tgtggcggta tagttgagca
     8461 aaggaacctg gggctgagtg atatcgaata cagggtgccc ctgtgcgtcg gcagcacata
     8521 ccgggtacaa ctcagattca aacagtttca ggcagcaaaa aggcgattgc ataagatctt
     8581 catcgcgaaa cgaaataata aagtcactct tgccttcgcg taacgtattt acggcctgga
     8641 caacatcaat ggcttcaaca tgatagacaa attctccgcc ataggcggta agtgaatgca
     8701 ccagtttggg taatacgctc agggatagtg agtgcgcggc tgcaatttta atgttgggaa
     8761 cgccgagtaa actctggccg tttaactccc ccaaattgca ttcaagctgc tgtaataagc
     8821 tgcgagtctg ggaatgaaag agcttgccct cttcggttaa ttgtaaaggg gtggtcgtgc
     8881 gatcgaataa aacaacgccg atcgctgcct ccagcgcctt aattcgtcgg ctgaatgcgg
     8941 gttgcgaaag attacgctct tttgccgcct gagaaaaatg gcgacaggct tccagtgtca
     9001 ggaaatcgta taaccatttt gtctcgatat tcttcattac catggcacct gtattttcaa
     9061 tccaaagaca taataaaaca tcccggcaat aaaaataatg ttctcaatta gcacaacctg
     9121 gcctttatca ttcgttttgt gcataacccc ttcatttccg gtattgacgt tgatatcaca
     9181 tttcgccaaa aaaacgaaat ttttccgtca gataaccaga atacatcatc actatggtga
     9241 ataagcggaa tgttccatag ctgcatcatg cgtgtcttgc atcgccttgt acaaccttgc
     9301 atttctcatc gcccatttcg actgttaaaa aagagagcag cagttattta ccgcgtattg
     9361 tcattaacgt tttgagaggc aggtattgtg aaaggtttaa ttggtgtact cggtggaatg
     9421 gggccggcag ctaccgtcga cctgtttaat aaatttgtaa cttttaccgc tgcacaatgt
     9481 gatcaggaac atattccttt aattatttct tcgatcccgg atattcctga ccgtaccgat
     9541 gcattaatgc atcatgggcg ttcaccatta cccgttatgc gtgattatat gcataagctg
     9601 gaagatgccg gggcagaatg tattgttatt ccctgtaata ccgcgcatta ctggttcaaa
     9661 gaattaaaag atgcttgtca tattgatata ttaagtatcg tagaaactac gatcaatgaa
     9721 gttcgcgcct gtggtaaaac ccgaattggc ttgttagcca ccaatgccac gctatatatg
     9781 gggttatacc aaaaaggaat tgagtcgctg ggatttacct gtgtcagccc cgatgccgac
     9841 ggtcagaaaa aagtgatgga aagtatttat tccttgaaag ctggcaatat agcgcacgca
     9901 caaaagttga tgaatgagca ggctgaaata ttattttccc gcggtgcaga aatccttgtt
     9961 cttggttgta cagaaattcc ggttattctg gcgcaagccg ttaaagagca gccttcccgc
    10021 tatattgact caacggcgtc actcgttcgt gccggaataa aatggtacga aaatcgtgtc
    10081 ggtaaacatt atcttttaac ccaataatca tttaaatcgc agccagaaag ttattcgctt
    10141 ttaactgaat tatatttata acggagaaca ttatggtttg gctggaaatt atcgtagtac
    10201 ttggtgcaat attttttggt attcgccagg ggggaatcgg tattggttta tgtggcgggc
    10261 ttgggcttgc cattctgact ctgggatttg gtctgcctat ggggtcgccg ccagtcgacg
    10321 tgatcctgat tattatgacc gttgttgtgg cggcctcagc cttacaggcc gcaggcggaa
    10381 tggattatct ggtacgcgtc gctggtaatt ttatgcggcg taacccgaaa tatatcaaca
    10441 taatcgcgcc gattatcacc tggtctatga ccattatggc cggtactggg tttattgttt
    10501 tttccacgct gccggtgatt gctgaagtgg cgaaagagtc cggtatccgt ccttcccgca
    10561 cgcttgccgg ttcggttgtc gcttcgcagg ttgcgatttc cggttcgccg atcagcgccg
    10621 caatggcggc tatgctcact attatggagg taaacggtgt cagttttatt caagtgatgt
    10681 cggtatgtct tcccacctct tttgctgccg cgatggtcgc cgcttttatt gcttcccgac
    10741 agggatgtga gttgcaggat gacgaggtct accttgagcg cctgcaaaaa ggactggtgc
    10801 aaaaatacga gaataatagc agcatcaagc caggtgcgac gctctccgtc ggattgttcc
    10861 tgctggcgac ggtcgctatt gttattcttg ctgccttccc ccaactgcgt ccgggttttg
    10921 atatcggtaa accgatggaa acacgcgata tcattattat ctgtatgctc tctgccgcct
    10981 gcctgatggt ggtgctgtgt aagacggcga ccgatgccat tattctcacc tccacattcc
    11041 gtgcggggat gagttcgctg gcggtaattc tcggcattgt aaccttaggc accactttta
    11101 tttatgcgca tttaactgag attaaagata ttgctggtga tattttgcag gcgtatccga
    11161 tgttgttggc gctggttctt tttggcactt gcgcgctgct ctactcgcaa ggtgcgacca
    11221 cgccgttaat tatcccgctt gccgtcgcgc ttgacgtgcc cacctgggca attctggcgt
    11281 cctatgtggc cgtcaccgga gtatttgtac tgccaacgta tccaacatca ctggctgcca
    11341 tggaatttga tactaccggc acaactcgtg taggcaaata tgtgctgaat catccattta
    11401 tgctgcctgg gttgggggga attattgctg gagtggcatt aggctttgtg attgcgccaa
    11461 tgattattta gcactatcaa acatcagtat cgatggttta tacacaataa agacagatat
    11521 ggcggaatat agtctgttgt aagcgccaac aaagaatagg ggaattccgc catgtctgaa
    11581 catcttcaca atatggtatt aagtgttgtc gtttaatgat ttatttcttg gttatgttgt
    11641 gtattagctg catctatcgc tttttgatac ctgaggtgta ctggcaatag cggacactac
    11701 catttgttct ttttttaagc agtcatctga tgatattttt ccctgaaggc tgccggggag
    11761 atattcccca gacgagagtg acgacgctga cgattgtaga aaatctcaat gtattcccgt
    11821 attactgaga tggcttcatc ccggttatta aaacgatagt agctcaggct tgcgtattac
    11881 cgtgaaggag atcggtgagt aacatcgatg gagatcggtt cgtgtcactt tcacagaacc
    11941 gtttttaaat taccttcact gatctccttc ggtcaacgga gattgtattt tccgcattga
    12001 ttctccttta agttcgatct tgatactgcc atgaaccagt cgatctagga tggcatccgc
    12061 atgtgtggag tctccgatca ttttgtacca gttttccacc ggcaactggc tcactacgat
    12121 gattgagcct cgttggtaca tcag
//
LOCUS       DAZFZC010000128        11870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFZC010000128 DAZFZC010000000
VERSION     DAZFZC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.129"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..630)
                     /locus_tag="ACHKXP_004316"
     CDS             complement(55..630)
                     /locus_tag="ACHKXP_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7649194.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKXP_004317"
     CDS             complement(699..1277)
                     /gene="terD"
                     /locus_tag="ACHKXP_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO7649195.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKXP_004318"
     CDS             complement(1326..2366)
                     /gene="terC"
                     /locus_tag="ACHKXP_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO7649196.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2389..2844)
                     /locus_tag="ACHKXP_004319"
     CDS             complement(2389..2844)
                     /locus_tag="ACHKXP_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7649197.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCVRIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2867..4024)
                     /locus_tag="ACHKXP_004320"
     CDS             complement(2867..4024)
                     /locus_tag="ACHKXP_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7649198.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPVPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4024..4605)
                     /locus_tag="ACHKXP_004321"
     CDS             complement(4024..4605)
                     /locus_tag="ACHKXP_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7649199.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            4928..5986
                     /locus_tag="ACHKXP_004322"
     CDS             4928..5986
                     /locus_tag="ACHKXP_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO7649200.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIESTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQNGLPVVPSWRVNTLAELKTHLAAPPFSDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASASESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDAVIQSARNTFSPVAV
                     RSITDVIVYPESLSNHLN"
     gene            5996..7138
                     /locus_tag="ACHKXP_004323"
     CDS             5996..7138
                     /locus_tag="ACHKXP_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO7649201.1"
                     /translation="MSDTLEDMIYRRTLSCGTIQVTRDQGDVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPATLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSSNALSERTSLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDILGKQSWGRLGMLAPAADLGHEVSVRKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQALYGSTTRSPIAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            7131..7904
                     /locus_tag="ACHKXP_004324"
     CDS             7131..7904
                     /locus_tag="ACHKXP_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649202.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYCETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVRWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            7906..8985
                     /locus_tag="ACHKXP_004325"
     CDS             7906..8985
                     /locus_tag="ACHKXP_004325"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO7649203.1"
                     /translation="MQPHDTFTGSYQPGDVEFLLKPVVIEMTPVEQKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAKRFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            8985..9941
                     /locus_tag="ACHKXP_004326"
     CDS             8985..9941
                     /locus_tag="ACHKXP_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO7649204.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHHDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDIAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            9952..11160
                     /locus_tag="ACHKXP_004327"
     CDS             9952..11160
                     /locus_tag="ACHKXP_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO7649205.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLALPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYARRVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFEGIEHIDPVEALGDHPVIRKMSSTF
                     IFQPIEPLTL"
     gene            11178..11645
                     /gene="terW"
                     /locus_tag="ACHKXP_004328"
     CDS             11178..11645
                     /gene="terW"
                     /locus_tag="ACHKXP_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO7649206.1"
                     /translation="MQLNTRQTRIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSGSTRSEAVEKLALHSVDELIKEYSTKKS"
BASE COUNT         2904 a         3117 c         3042 g         2807 t
ORIGIN      
        1 cggatcgata cccaaaaaaa accccggcat tgccggggtc agactcagcg tcttttagac
       61 attgacgccg tgctgggaag caagcgctga cagaccaccg gcaaaaccct ggccaacagc
      121 tttaaacttc cactcagtac catggcgata cagttcaccg aagaccattg cggtttcggt
      181 tgaggcgtct tcagacagat cgaaacgggc aatttccgtc ccgttgtcgt tgttgtaaac
      241 gcgcatgaag ctgttgctca ccatgccgaa gttttgttta cgcgcttctg catcatagat
      301 ggtaacagca aataccagtt ttttgatgtc tgctgagact ttggtcagat cgattttgac
      361 ctgctcatcg tcgccgtcgc cttcaccggt acggttgtcg ccctggtgct ctactgcgcc
      421 atcagggctg gttttattat tgaagaaaat gaaatgggca tctgacagta ctttaccgtc
      481 ttcacctact gcgaatacgg aagcgtccag atcaaaaccc tgaccatcgg ttacacgggc
      541 atcccagccc aggccaacca tagcgacatt catggttggt gcttctttgg tcagagatac
      601 gttgccgcct tttacgagag aaactgccat ttttagctcc tgcaaacagt tgaatgaggc
      661 tgaattaaca gccccagata tgaaaagtta cttttcgatc aggacgcgtt aatgccgtac
      721 tgagcacata cagatgccag accaccagca taaccctggc ctactgcgcg gaatttccac
      781 tcaccattgt ggcgatacag ctcgccgaac agcatggcag tctcagtgga cgcatcttcg
      841 gtcagatcgt agcgagcgac ttcagtctgg ttatcgtcat taaccagacg aataaacgca
      901 ccggatacct gaccaaagct ctggcgacga gcctgagcat cgtggatggt cacaacgaag
      961 atgatcttgt caacttcaga cgggacggcg tccagtttaa ttttcagcga ttcatcatca
     1021 ccatcgccct caccggtgcg gttatcgccg gtgtgcgtta cggaaccgtc ggatgacgtc
     1081 aggttgttat agaagatgaa atctgaatcg ccgcgcactt tgccgtttga ggccagcagg
     1141 aatgctgaag catccaggtc aaagtcctga ccgtctgttg aacgcgcatc ccagccaagg
     1201 cccaccagga catttttcat tgacggagct gctttactca gggagacgtt cccgcctttg
     1261 gaaagagaaa cactcataaa ataacctctt cgattagtaa ttgttcaggt taacacttaa
     1321 ggggattaac tccccttttc ctcagattca ggtttgcccg ggaacatgac gcttgcgaga
     1381 atgcccagcg ccagtacacc cagtacaaca tacaggctgg ttgttgccgc gatgctgtaa
     1441 ccatgatgcc agatgtgatc ggtcgcattc aggccgagtt ttgccgcgat gaagaacagc
     1501 agcacgataa cggccttctc cagatgaacc aggtactgtt tcagtgcctc aaggacaaaa
     1561 tacagagtac gcaggcccag gatagcaaac atcatggcac tatagacgat aagcggttca
     1621 cgactgacgg caatgattgc cggtaccgag tcaaacgcga acattacgtc cgagagttca
     1681 accacagcca cacacaggaa cagcggggtc gcatacagcg ccgctttttt accacggcca
     1741 atggtgacat cgctgttttc tggtttcgcc agttcagcat ccacttcctt ctggttaagc
     1801 aggaaggcat gccctctgag cttcggccag atagggaaga agcgtttaac catacggtaa
     1861 gccagatgct gggagtaatc ctcaatttca tcatcgtcat caccgctttt aagcatcatg
     1921 accgccgtcc aggcaacgat aatagcgaag acaacttcaa cgtacggccc cagactcagc
     1981 aggctcgtac cgatggcgac aaagatgccc ctgaagacga tggcaccaat gatcccccag
     2041 tagagaacac ggtggcgata acgatccgga acggcgaacc aggagaagat tgccatcatg
     2101 acgaacaggt tatcgaccga cagcactttc tccagcgcat aacccgtgac aaacagactg
     2161 gcaacctcag caccgtggtg gatatagagg aaaccggcaa atgccatcgc aaccacaacc
     2221 cagaatacgg accagagcgc agcactcttc agcgagattg gcttgtcatc acggtgcata
     2281 aacaggtcaa taaagatggc accgactgaa agcgcaataa aaacaatgac cgtttcagtc
     2341 gggaagccga tgtgtgtgga aaccatattt tacccttagg gatgtggatc agaggccaaa
     2401 ttcagccggg tcgaagccca acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg
     2461 atcgaagtta ccgtcacttt tcgcaacggc aataccaaca cgtaaggcca gctgagcggc
     2521 ctcaggctga tctttcagcg ccaggatgta tttcatggtt tcgcccttgc caatttcaac
     2581 atcgaaatcg aagcttgaaa ccagtttatt gaagaactca atcacctcat tggtatcgaa
     2641 gaccttcagc tcttctgaag agcgcagaaa gcccatcatt ttctgctttt cttccgaact
     2701 tacgccgtca ctcgatacgg caatacggac acatacagca acggtgccct gcatgaattt
     2761 tttgtttttg aaacggccaa cctggcgggt cagttcgtca cggcctgagt taatggcacc
     2821 tttaactttg tcgaaaaagc tcatacctgt ttcctttgtt gctgtgttat ttagaaccgg
     2881 cgctccagcg aaatccccag ccaaatgccc ggtccatttc gtcctggcct ttgaagtact
     2941 ggttaattcg ctcgacttta attgcgccgt tttcgtttac gagtctggca atggcgcaca
     3001 atgtgcgacg gttctcacct tcggtcaggc gggtctcaat gggcggttga tccggcacat
     3061 gaatggtgac tacaccatca gtcttgtccc agctgggaac accttcataa ataaaggcgt
     3121 aaatcagcac ttcgcggata tgcttccatt cacgtccatt gatatgcagc cactcgccgt
     3181 ctgatacatc acccgtccgg tcatcgccct tgagctgaac atacggttca tcgcgataat
     3241 caccgaatgc attaccgagc gcctgaatga ccgatttata cccgtcctga agctcaacaa
     3301 aggcccccag atccaggtcg attcctttgg agccgaacat acctgcaaag cctgatttgc
     3361 tactgccccg gtgccagttc aggttgatcc ggatttcacc gaagttatcc cgtttggtca
     3421 ggctgattgc aggcttctct tttgtcagcg ataccttgct caggctgatt tttgtctcaa
     3481 ctggcggagg ggttacaacc ggaggagtag gggctgcggg aaccggttcg tcggcaatat
     3541 ttacgccgaa atgctctgcc agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc
     3601 ggaatttcca gtcattatta cgacggtaaa attcccccag aattaaggct gcttcctgac
     3661 ggccgctcag ttcgacactg ccgctaacca ggccggtagc cccttgctcc acgtcgatgg
     3721 aaagattacg aagaccggac accgtctgcc caccgtcaca ggtcacggtg aacgcaattt
     3781 tctggacatc aggtttcagc cggttgagcg cgacagtaaa ggttgagtac tggccttcac
     3841 tgaccaggct gacggtacca tcatcgttac gtggctgacc ataaaacacc atgtccgcat
     3901 caccctgcac cttcccgtcg gcatacaggc gaaacgccga agcatccacc tggccgcccg
     3961 aggtgatccg gacccgcaac tcctgggctg gaacgggggc attgcccccc ggcgtcaggt
     4021 tcattagcga ataaccgctg aaacgatatc ggagtgcata tcgtcaattg tgcggccgcg
     4081 acaggcatgg ccaagggcgg taaagtccca gttgccgtta ttacggcgca gcgaagaaat
     4141 aacgatcccg gtatgtgagc cctgctcagt cagcttgtag cgagccagct ctttgccggt
     4201 ttgatcgaca acgcggcaga aagcgttttc gacgtcattg aacgactgac cacggaagct
     4261 gtttaccgta aacgccaggt attcaacgtt tgcaggcagg cgggagaggt tgaccttaat
     4321 cacttcatcg tcaccgtcac cctcaccggt caggttatcg ccactgtgca caacagcgcc
     4381 gcaggtggat ttcagtttgc ggaaccagat ggtgtcaatt gttttacctg tgctgtccat
     4441 taatacacag ccagcatcga gatcgattga gtcgttgcct ccaaacaatc ctccaagaag
     4501 accttttttc ttaaccggat cccaaccgag accgaaatga agctggctta atgcagatga
     4561 ttctttgctg agtgataccg tctggttctt aactaatgaa accatgtttt attccttaaa
     4621 atggtaatga ccgagacaat caaaggtata tttagtaact gacgaagaaa atcatatcat
     4681 gatgaagttc tctgacaagg gaaatttttg tggttttctg taaaaacaca aaaagaaatc
     4741 cttttcaggg tgttagtttt tctatgttta atttaaatat caatgtgtta ccaggaatcc
     4801 aaaaaacact ggcgcactcc ataaccacag gagttattaa tcatattatg attgacatgt
     4861 agcttagaca ctcatagact cacttctcaa tcataaattt gcatggcgtt ccggataatt
     4921 aaaaaaaatg aacaagaaaa tttggtttat ggaaggttta tcctcccagc gagatattat
     4981 tcagggggta aaatcatttg cacaaaaaaa taattttgcc attaccgttt ttgcctccca
     5041 ccgtaacgaa agaaatgaaa tcctttccgt tgccgattat tctttgactg aacctgaaga
     5101 tcctcaaaaa cgtcttcagt ttatccagga aaccattcag acctacggca tccaccatat
     5161 tcatactggc cgtaacagcc agtggtttga agaacaccgt tcagccattg aatcgaccgg
     5221 agccaccctt actaccggtg caacaggcgt cgactggtta actctggctg acgaaaaagt
     5281 tacttttgct cagtttatgg agcaaaacgg gctcccggtc gtaccgtcct ggcgggtgaa
     5341 tacgctggca gaattaaaga cgcacctcgc ggccccgccg ttctcggaca gcccggtatg
     5401 cgtgaagccg gtgacgggta tctatggcat gggattctgg cgctttgatg acagtgcttc
     5461 gcccatggcc gtctttaatc atcccgaaca tcgtctggtc agtccgcaac agtatattgc
     5521 agcagcatcg gcttctgagt cgtttaaacc ccttgttttg atgccgtacc tgccaggccc
     5581 ggaattttcc gtcgatatcc tcgcggataa gggcgaaata ctcgcagccg tgggacgccg
     5641 taaggaaggg gctatccagt atctggtaaa cgaaggaagc gcctgggaac tggcttgtga
     5701 ctgcgcccgg gttatgaagg ccgacgggct ggtgaatgtt cagacgcgaa acgatgtgaa
     5761 tggcaacccg gtgctgcttg aaaccaacat gcgtccgtca ggcggggtgg gttataccct
     5821 tcacagcggg gtgaaccttc ccgggttgtt tgctgccttt aagctcggtc tgatgtctga
     5881 agatgcggtg atccagagcg ccagaaatac cttttctccg gttgcagtga gatccatcac
     5941 agatgtcatc gtttacccgg aatcactctc taaccatctg aattaaggcg tgtttatgtc
     6001 tgataccctc gaagatatga tttatcgccg taccctctca tgcggtacga tacaggtaac
     6061 ccgcgaccag ggtgatgttt cgctcgatga cctgtttgat atcgctgaaa gacgtaaccc
     6121 gaaacgcgcc tttctgtttg tcagcaaagt actcggcagg cacattcccg tgccaccctc
     6181 agtcatgcgg caggcctaca gacagcttgc cagccagttc cccgcaacgc tgacagggcc
     6241 cgtacttttt atcggtatgg ctgaaaccgc cgttgggcta ggggccggtg tgtttgatga
     6301 agtgcgccac cagcatcgtg aatctgtcta tctgacctct acccgtcacc cggttgatgg
     6361 cacgttactt tgcgagttca aggaagaaca cagccacgcc accgatcatc tgatctatct
     6421 gccagatgat gaagagaaaa gacgtcgtgt taccaacgca cgaacgctgg ttttgattga
     6481 tgacgaggca accaccggta atacctttat taacctgctt tctgccctgc gtaataccgg
     6541 caagcttcaa catattgaac aggttatcgc cgttacgctt accgactgga gtagcaacgc
     6601 cctgtcggag cgcacatcct tgcccgtcac ttcggtttcg cttgtaagcg gtcagtgggg
     6661 atggacccca ttacctgatg cacctgtccc ggacatgccg aaagtcaacg taacttcacg
     6721 cggagaatgg gacatcctgg gaaaacagtc ctggggtcga ctggggatgc tcgcacctgc
     6781 ggccgatctc ggccatgagg tctccgtccg caagggggaa cgtattctgg ttctcgggac
     6841 cggggaattc gtctgggagc cgttcctgct tgctgaacgg ctcgaagctg ccggagcaca
     6901 ggcactttat ggttcgacca cccgctcccc tatcgccgtt ggttatgcca ttgagtccgc
     6961 catttccttt acggataact acgggctggg catccccaat tttgtctata acgtcgccca
     7021 ccagcagttt gaccgtattc ttgtgtgtac tgagacaccc gcagaaagta ttgacacgca
     7081 gcttcttaaa gccctggctg aggttgcgcc cgtcgtggag attgttacct atgaataaac
     7141 ccgttgttct gagcgacctt gacgacacgc tctttcagac ccgtcgtaaa atggtggacg
     7201 agctggccct ggagccattc cgtaccggtg ccgttgatcg caccctgaat ccacgaagct
     7261 ttatgacgga agaacagtcc atgttggtgg actggctcct ggagcaggct gaactcatac
     7321 ctgttaccgc ccggggaact gaagaaatca gccgcgtccg gatccctttc cactcctggg
     7381 caatcaccac gcacggggcc gtcattctca cgcctgaagg caaacccgac gaggagtgga
     7441 aggcccatat gcttggccag ctggctccct accaggaaaa gctgacatcg atgcagcgtc
     7501 tgatcactga aatgatggac gcaaaaggga tcaatgcctg ggcaaggctg aacttcgaat
     7561 actgtgaaac ggcggtttat atggtgatga agcaccgcga cagcacccgc cttgacgagc
     7621 tcaatgccat tgcagatgag atagaaacgg tgtttccgac cgagggcttc tacatccacc
     7681 gcaacagtaa taacgtggcc tggcttccca ccccggttga gaaagggctg gcagtcagat
     7741 ggctgcttga aaaacttcgg gctgaacgcg gagtcttccc cgtaattggc ctgggcgaca
     7801 gcctgagcga tcatcgtttt atgaaactgt gcagctggtt tggcatcccg cgtcagagtc
     7861 agtttgcaga tgccatttca cagcgaattt ttggagaaaa ttaaaatgca gccacatgac
     7921 acatttaccg gctcatacca gcccggtgac gtggaatttc tgctaaagcc ggttgtcatt
     7981 gagatgacgc cggttgagca aaaagaagag ctgattcagt cagggaagaa acattattcg
     8041 gacatgctca gtcaggagcc agcgccaaca caatggcatc ttgatttgtt tcaccgggcg
     8101 ctggatcggg gcgcagagag acttgcaaaa gaagtcacac agctcgctat tgctctggcc
     8161 aaacgctttg gtgatgagcc cattgtactg gccagtcttg tcagggctgg cgtgccgctc
     8221 ggcgttatgc tgcaccaggc cctgcgtgac atggggaaaa cctcatggca ttacggtatc
     8281 agcattatcc gggatcgtgg aattgacggt gcagccctcg acgtcattga agagcggcat
     8341 ggtaccagcg gtattgtctt tgttgacgga tggacaggta aaggcgcaat taccggagag
     8401 cttgtacgcg cactgaaaga tcgcccgggc tatcctgagc agccgcgact ggttgtcctg
     8461 gccgatccct gtggctgctc ctggcttgca gccagtgatg atgactggct cattcctttt
     8521 ggcatcatgg gggcgccggt atcaggcctg atctcccggt cagtatggtc ctctgaagga
     8581 ttacacgggt gcatggtttg cgagcatctc agtgaattcg aatgcagccg gatgcttgcc
     8641 gataccgtcg ctcatttccg taagaagtta acaccgtcat ccctcgctcc cctgagctgg
     8701 aatacggaat cagcccgggt cttatggcag acaagtcgcg acgttatcgc gttcctggcc
     8761 gatgaattta aagtggacag cgtcaatcgt attaaacccg gtattgctga agcaacccgg
     8821 gctgtattac gtcgggtacc ggaccatgta tttgtgcgtt ctattgacga cccggacgtt
     8881 gccttgcttg tagggcttgc tcgtgaaaag ggaatagttg ttacagaaat ggggggaacc
     8941 ctcggccagt atcgggctgt aaccattatc aagaaggtac tctgatgaaa aatcgtcttt
     9001 ctccctggaa tctgggagcc acgctataca tgcctgcaac acgggaagat attgctgatg
     9061 ccgtcctgca cgggaaaatc ccgggcttgc gctctctggt gatttgcctg gaggatgctg
     9121 tcagtgaagc agacatccct gtcgccctca aaaatctgga gcacctgctg cacgagctca
     9181 gtaacagcat gagtagcctg ggtaaaaatg actggcctct ggtgttcatt cgcccccggc
     9241 atgccgaaat gggcagatgg ttaaaagcgc atcatgatct ttccgctgtc gacgggtttg
     9301 tgctgcctaa atttaccctc agttcgttag ctgaatggtg ggatatcatg gtcgggactc
     9361 acctgtgcat gatgccaacg ctggaaacag aagatgtctt tgacgtggtt cagatgcgcg
     9421 agctggctac ccgtctggag gaacatccct gccacgaccg cattattgcg cttcgaatcg
     9481 gcggcaacga tcttatgaac gttgtgtcgc ttcgccgtcc ccgggacctg acgctgtatg
     9541 acagcccgat gggctacgtc atcaaaatgc tcgtttcggt cttcggcccg cgtgattttg
     9601 ccctgaccgc gcccgtatgt gagcatattg acgatcatgc cgttatggcc agagagctgg
     9661 ctcttgatat tgcacacggg cttgttggga aaacggccat tcacccgggt cagatagagg
     9721 tcattcaaaa cgcgctgatg gtcactcagg gtgagcattc tgacgctctg cggatcctga
     9781 actctaccca ggccgtgttt aagtcgcagg gagcaatgtg tgaacccgcc acacatcgcc
     9841 gctgggcggc tggcattctg gacagggctc gtttttatgg gttacagaac gagcaaagcg
     9901 ctgatggaat cagattactt accgtgaccc agcatcatta aggaaatcat aatggggttc
     9961 aggttcagaa agtcgatcaa cattattcct ggcgttcgcc tcaacctgag taacggtgca
    10021 ccgagcctga gtgtcgggcc gagaggtgct tccgtttctt ttggtagccg ggggacctat
    10081 gccaatctgg gcttgcccgg taccgggctg agttaccgta cccggcttga ccgggccgcg
    10141 cgttccagag gtgaaaaccg gacggcaacc gacccggggc tcagacaggc gcttgagcag
    10201 aaagccgctg aactcatgtc agcggtaacc gcaatccgta atattcacga gctgacgccg
    10261 gatccaaaaa caggcatcag ctgggcagag ctggaagcag tatacctgca taacagaacg
    10321 tcgccttttc aggttccggc accggtgcgt ccagaaaagc cagactacct tgcattgccg
    10381 gaaaagcctg ccgagagcga aggcattagt tttttgggta aatggtttga atcggaatca
    10441 gctaaagctg agcgccacgc cgaaaatctt cgccggtggc agcaggagct gattgatgtg
    10501 gagcgtgaga atacccttcg acagcaccgg taccagcaac aacggacggc ctgggccgaa
    10561 cagtatgcaa actggaagtt tgaagctgaa gaacatgaaa aacggctcgc cacggctcag
    10621 gcagatgccc ggcagcagtt ccggacagac gccgcgtttt tcgaatcata cctggcgggt
    10681 gtgctggcag aaactgaatg gccgcgtgaa acgcttgttg catttgaagt aaagccggag
    10741 ctatcagcag tcctgctgga cgttgattta gctgagattg aagatttccc tgataagatt
    10801 tacggcgtta atgcccgggg aacggagctg acggaaaaag ccatgacgca aaaagccgta
    10861 cgcgaaaact atgcccgccg cgtccatggc tgcttgttcc gcctggtcgg tatcgtttta
    10921 catacgctac ctttcgacaa cgtgattgtg tcaggcttta cgcaacgggt cagtaagcgg
    10981 accggctatc tggaggatga gtatatcctg tcctgcaaat gcactcgcag ccagatgtcg
    11041 tcagtaaatt ttgaaggcat agaacacatt gatccggttg aagcgttagg cgatcacccg
    11101 gttattagaa agatgagcag taccttcatt tttcagccta ttgaaccact aaccctttaa
    11161 gcaggctaaa tcttacgatg caattaaata ccagacagac ccggattttt aagcttgcca
    11221 acttactggg caccggcaag cccgtttccg ctgccgacat tataaccagt ctggaatgct
    11281 cagagccgac attaacccgg gccctcaaag agctacgcga gtcatattct gcggagatca
    11341 aatacagcaa ggcaggtcat tcctatcatc tcgttaatcc gggccagctg gataaaaaga
    11401 cccttcgccg gatgaatgaa gcgctcgcac agaatgctga actgaaaacc ggtgagtcca
    11461 cagggaaggt cgtactcgat aaggataaaa aaacagctgt gtcattatcg ctacgcatgc
    11521 ggattctaag aaaaattgac cgtctggctg cgctgagtgg ctcgacccga agtgaagcgg
    11581 tagagaagct ggctctgcac tccgttgatg aactgataaa agaatacagc actaaaaagt
    11641 cctgacgcta aatctcagac cggggtgaaa actccggttt tgtcatattt cacctctgta
    11701 ttggcgtgag ggagttttga tctattctcc ttccgcaatg tcagcagcgc cttactgcct
    11761 acaaaagctg gtgtcttgac atcctcatgt gctacacatc aatctttggg cgatttacac
    11821 tccattcatg cagatctgtg atgtatgatg atttttatac ataagaaagt
//
LOCUS       DAZFZC010000129        11833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFZC010000129 DAZFZC010000000
VERSION     DAZFZC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.130"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..711
                     /gene="ydeO"
                     /locus_tag="ACHKXP_004329"
     CDS             <1..711
                     /gene="ydeO"
                     /locus_tag="ACHKXP_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO7649207.1"
                     /translation="ILDKDYAFSDGEILMVDNAVRTNFEPYERHFKEIGFNENTIKKY
                     LQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFA
                     ACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTT
                     FSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYR
                     CQSHTGMNTGNTMNALAI"
     gene            1113..2795
                     /gene="ydeN"
                     /locus_tag="ACHKXP_004330"
     CDS             1113..2795
                     /gene="ydeN"
                     /locus_tag="ACHKXP_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7649208.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2847..4004
                     /gene="ydeM"
                     /locus_tag="ACHKXP_004331"
     CDS             2847..4004
                     /gene="ydeM"
                     /locus_tag="ACHKXP_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7649209.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRIPAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3970..4092)
                     /locus_tag="ACHKXP_004332"
     CDS             complement(3970..4092)
                     /locus_tag="ACHKXP_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649210.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            4295..5980
                     /gene="yddA"
                     /locus_tag="ACHKXP_004333"
     CDS             4295..5980
                     /gene="yddA"
                     /locus_tag="ACHKXP_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO7649211.1"
                     /translation="MITIPITLRMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            6018..8390
                     /gene="yddB"
                     /locus_tag="ACHKXP_004334"
     CDS             6018..8390
                     /gene="yddB"
                     /locus_tag="ACHKXP_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO7649212.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGRLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8447..11230
                     /gene="pqqL"
                     /locus_tag="ACHKXP_004335"
     CDS             8447..11230
                     /gene="pqqL"
                     /locus_tag="ACHKXP_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7649213.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYVFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLPTHMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            11592..>11833
                     /locus_tag="ACHKXP_004336"
     CDS             11592..>11833
                     /locus_tag="ACHKXP_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7649214.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         3511 a         2571 c         2534 g         3217 t
ORIGIN      
        1 attttagata aagattacgc cttctctgac ggcgagatcc tgatggtaga taacgctgtt
       61 cgtacaaatt ttgaacctta tgagcggcat tttaaagaaa tcggatttaa tgaaaatacc
      121 attaaaaaat atctacaatg cactaacatc cagacagtga cggtgcctgt tcctgcgaag
      181 tttttacgtg cttcaaatgt accgactgga ttgcttaatg aaatgattgc ttatctcaac
      241 tcggaagaac gcaatcatca taatttttca gaacttttgc ttttttcttg cctgtctatt
      301 tttgccgcat gcaaaggttt cattacacta ttaactaacg gtgtgctatc cgtttctggg
      361 aaagtgagaa atattgtcaa catgaagctg gcgcacccat ggaagctgaa agatatttgt
      421 gactgcctgt acatcagtga aagcctgttg aagaaaaaac ttaagcaaga gcaaacgaca
      481 ttctcacaga ttcttttaga tgcaagaatg cagcacgcaa aaaatttgat acgcgtagaa
      541 ggttcagtca ataaaattgc cgaacaatgt ggttatgcca gtacatctta ttttatttat
      601 gcgttccgca aacatttcgg caacagtccg aagagagttt ctaaggagta ccgttgtcaa
      661 agtcacacgg gtatgaatac gggcaacacg atgaatgctt tagctatttg attatttgct
      721 aacgagtagt caaccacaca cgctgcgtaa gaattaaatg gggcagccat tccctgcccc
      781 gcgttgtttt taggcgatat atttattgaa ataaataagt gacatccatc acatatttat
      841 gcacttgcat aacctgttgc atgattattt atgatctcaa ttctgcattt tgtcagtaaa
      901 atgcaataat ttattaaata tcaataaatt agttgtttat cggcgagaaa ttacttaata
      961 gaacagaaag taatgtcaac gctttatgga ctgttttttc ccttttttta gctaaatctg
     1021 ctatctcttt atgtgactaa cttcacttac atccacttat ttctcttcgt aaaattactt
     1081 tggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag aaaagtgtcg
     1141 taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct gctcatgcgg
     1201 cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac tttacgccga
     1261 cagaatacag taccaaagga aagccaaata ttatcgtact gaccatggat gatcttggtt
     1321 atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa aatcgtgaag
     1381 ttgtcgatac ctacaaaata gggatagata aagccattga agctgcacaa aaatcaacgc
     1441 cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat gtggcacacg
     1501 gtgtttccgg cccctcccgc gccgcaataa tgaccggtcg agctcccgcc cgctttggtg
     1561 tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc ttgcctgaat
     1621 tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg tcaaaaatca
     1681 gtaatgtgcc ggtaccggaa gataaacaaa cgcgtgacta tcatgacaac ttcaccacat
     1741 tttctgcgga agaatggcaa cctcaaaacc gtggctttga ttactttatg ggattccacg
     1801 ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa cgtgtccccg
     1861 caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt gatcgtgcca
     1921 aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac ctgccaaatg
     1981 ataatcctgc accggatcaa tatcagaagc aatttaatac cggtagtcaa acagcagata
     2041 actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc gaacaactga
     2101 agaaaaacgg acagtatgac aatacaatta ttctctttac ctccgataat ggtgcggtta
     2161 tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag acctatcctg
     2221 gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc ggtaattatg
     2281 acaagctgat ttccgcaatg gatttctacc cgacagctct tgatgcagcc gatatcagca
     2341 ttccaaaaga ccttaagctg gatggcgttt ccttgctgcc ctggttgcaa gataagaaac
     2401 aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg tttgacgagg
     2461 aaaatattcc attctgggat aattaccaca aatttgttcg ccatcagtca gacgattacc
     2521 cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga aataacgatt
     2581 attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaaactg acggatctac
     2641 agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg caaggcgtgg
     2701 taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag gagaagttta
     2761 acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg gcggattttt
     2821 ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc agttttcaat
     2881 gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt actcatgaaa
     2941 aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgca gcgtctggca
     3001 atcaggtcta ttttacctgg caaggcggtg aacccactct ggctggcctg gattttttcc
     3061 gtaaagttat tcactatcaa caacgctatg caggccaaaa acgtattttt aatgcattac
     3121 aaacgaatgg cattttattg aataatgaat ggtgtgcctt tctcaaagaa catgaatttc
     3181 tggttggtat ctcgatcgat ggcccccagg agttacatga ccgttacaga cgcagtaatt
     3241 caggtaacgg tacttttgca aaagtgatag cagccatcga gcgtctgaaa tcatatcaag
     3301 tagagtttaa tacgttaacc gtcattaata acgttaatgt ccattaccct cttgaggttt
     3361 atcatttttt aaaatctatc ggcagtaaac atatgcaatt tatcgaattg ctagaaaccg
     3421 ggacgccgaa tattgatttc agtggtcata gtgagaacac attccgtatc attgattttt
     3481 ctgtgcctcc cacggcttat ggcaagttta tgtcaaccat ttttatgcaa tgggttaaaa
     3541 acgatgtggg tgaaattttc atccgtcagt ttgaaagctt tgtcagccgt tttttgggga
     3601 atgggcatac cagttgtatt ttccaggagt cctgcaagga taatctggtt gttgaaagta
     3661 atggagacat ttacgaatgc gaccattttg tctatccaca gtacaaaatt ggaaacatta
     3721 ataaatctga actcaaaacg atgaacagtg tacaactgac agcgcaaaaa aaacggattc
     3781 cagcgaaatg tcagcaatgt gcatataaac ctatctgcaa tggaggttgt cctaagcatc
     3841 gtattactaa agtaaacaat gagactgttt cttatttttg cgaaggttat aaaatccttt
     3901 tttcaaccat ggtaccttat atgaacgcca tggtagagtt agctaagaac agagtaccgc
     3961 tttaccacat tatggatgtt gcaaaacaaa tggagaataa ttaataacgc tctcttttaa
     4021 ctccattctt agcctccttc ttatgacctt aacaaggcac ccgacataaa ttcctgtcgg
     4081 gtgtttcgtc atcatcatct tcccttgcga aaatcctggc ttacgtggta tgcatcaggg
     4141 cattagcaat atccttgatt atcaatagca taaccataac cactgaaaat gaaatattcc
     4201 tttatatttc atattgttaa atgatactta aataaatttc atcagtaaat cattgccata
     4261 gataatcatt ggatggcaca atcaattttc gctaatgata accattccca ttacgcttcg
     4321 tatgttgata gcaaagtatc tctgtctgtt gaagcccttt tggctgcgta agaataataa
     4381 aacttcagtg ttgttaatca ttattatcct cgcaatgatc cttggcgtgg ttaaaattca
     4441 ggtctggtta aacgactgga ataatgattt tttcaatgcg ctaagccaga aagaaaccga
     4501 taagctttgg cagcttgtcc tttggttccc tgccctgctg gggatctttg tattaatatc
     4561 cgtaaacaaa acatggttaa ttaaactgct gactattcgc tggcgcgaat ggttaacaga
     4621 ttactacctt aatcggtggt ttgcagataa aaactactac ttcacgcaga tctacggcga
     4681 gcataaaaat acagataacc ctgaccaacg tatcgctgaa gacattctct tactgatcag
     4741 caaaacgctc agtctttcat ttggcttcat ccagtcactt agcatgctga tcacctttac
     4801 cgttattctt tggcaaagcg cgggtacgct ctcattcact gttggtggaa cagaatggaa
     4861 tatccagggg tatatggtct ataccgtcgt gctcattgtg atcggtggaa ctttatttac
     4921 ccataaggtg ggtaaacgta ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac
     4981 atttcgaaca aatcttgtgc agcataataa gcaggcagaa ctgattgcgc taagcaatgc
     5041 tgagtctcta caacgccagg agttgagcga caattttcat accattaaag agaactggca
     5101 tcgtttaatg aatcgtcagc ggtggcttga ttactggcag aatatttatt cgcgctcgct
     5161 tagcgttctt ccctactttc tgttattgcc gcagtttatt agcgggcaga tcaatctggg
     5221 cggactgatg aaatcgcgcc aggcatttat gctggtatcg aacaatttaa gctggtttat
     5281 ttataaatat gacgaacttg ctgaactggc tgcggttatc gatcgcttgt atgagttcca
     5341 tcaactcact gaacagcgcc ctacgaataa gcctaaaaat tgccaacatg cggtacaagt
     5401 ggctgatgcg agtattcgta cgcctgataa taagatcata ttagagaacc tgaactttca
     5461 tgtttcgcca ggcaaatggc tattactgaa aggctactct ggtgcgggaa aaaccacact
     5521 gcttaaaaca ttatcccact gctggccgtg gtttaaaggt gatatttctt ctcctgctga
     5581 cagttggtat gtgtcacaaa caccgttaat caaaaccggc ttactgaaag agattatttg
     5641 taaagcactt cccctgcccg tagacgataa atcgttgagc gaagtactgc atcaggttgg
     5701 tcttgggaaa ttggctgcgc gtattcatga ccacgatcgc tggggagata ttctttccag
     5761 cggcgaaaaa caacgtatcg ccctggcacg attaatttta cgacgtccga aatggatatt
     5821 tcttgacgaa actacctctc atcttgagga acaagaggct atccgcttac tgcgtttagt
     5881 gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca catcaacccg gtgtctggaa
     5941 cctggccgat gatatttgtg acattagcgc ggttttataa aaacaataaa agccctgctg
     6001 tacggagata taaataaatg aagcgagttc ttattcctgg cgtcatttta tgtggcgctg
     6061 atgtggcgca ggccgtcgat gacaaaaaca tgtacatgca tttttttgaa gagatgacgg
     6121 tctatgctcc tgtccctgta cccgtaaacg gcaacacgca ttacaccagt gaaagcatcg
     6181 agcgtttacc gaccgggaat ggcaatatca gcgatctgct gagaaccaac cctgcggtac
     6241 gcatggattc aacgcaaagt acctcgttga accagggaga tattcgccct gagaaaatct
     6301 ctattcacgg tgcgtcgccc taccagaatg cctatttgat tgacggtatt agtgcaacta
     6361 ataacctgaa cccagcgaat gagtccgatg ccagtagtgc aaccaatatt agcgggatgt
     6421 cacaggggta ttatcttgat gtcagcttac tggacaatgt gacgctttat gacagttttg
     6481 tgccggttga atttggtcgc ttcaatggcg gggtaattga tgcaaagatc aaacgcttca
     6541 acgctgatga tagcaaggtg aaattgggtt atcgcactac gcgttcggac tggttaacat
     6601 cgcatatcga tgagaataac aagagcgcat ttaatcaagg ctcttcagga agtacttatt
     6661 actccccaga ttttaaaaag aacttttata ccttgtcgtt taatcaggaa ctcgctgata
     6721 acttcggcgt taccgccggt ttatcgcgcc gccagtctga tatcacccgc gcggattatg
     6781 tttcgaatga cggcattgtc gccggtcggg cacagtataa aaacgttatc gatactgcat
     6841 taagcaaatt tacctggttt gccagcgacc gctttaccca cgatttaacc ttaaaatata
     6901 ccggctccag ccgtgattat aataccagca ccttcccgca gtctgatcgc gaaatgggta
     6961 ataaatccta tggtctggca tgggatatgg atacgcagct cgcatgggcc aaactgcgta
     7021 ccaccgttgg ttgggatcat attagtgatt atacccgtca cgatcatgac atctggtaca
     7081 ccgaactttc atgtacatat ggtgatatta cagggcgttg cacccgtggc ggattaggac
     7141 acatttccca ggctgtagat aattacacct tcaaaacacg cctggactgg caaaaattcg
     7201 ccgtgggtaa tgtttcgcat caaccctact tcggcgcgga atacatctat tccgatgcgt
     7261 ggactgaacg ccataaccag tctgaatcct atgtgattaa tgctgccgga aagaaaacta
     7321 accataccat ttaccataaa ggtaaaggcc gcctgggaat tgacaactac acgctgtata
     7381 tggcggatcg cattagctgg cgtaatgtgt cattaatgcc cggcgtgcgg tatgactatg
     7441 acaactatct gtcaaaccac aatatctccc cgcgctttat gacggaatgg gatatttttg
     7501 ctaatcaaac ctcaatgatt acagcaggtt ataaccgtta ctatggcggg aatattcttg
     7561 atatgggatt acgtgatatc cgcaatagct ggacggaatc ggtatcaggt aataaaactc
     7621 tgacgcgtta tcaggatttg aaaacgcctt ataacgatga actggcaatg ggattgcagc
     7681 agaaaatcgg taagaacgtt attgcgcgtg caaactatgt ttaccgtgaa gcgcatgatc
     7741 aaatcagcaa aagcagtcgt accgacagcg cgactaaaac caccattact gaatataaca
     7801 acgatggcaa aaccaaaacg cattcgttca gcctcagttt tgaactggcc gaacccctgc
     7861 atatccgcca ggtagatatt aacccacaaa ttgtctttag ctatatcaag agcaagggca
     7921 acttgtcgtt aaacaatggt tatgaggaga gcaataccgg tgataaccag gtggtttata
     7981 acggtaatct ggtctcttac gatagcgttc cagtggcaga ttttaataac ccattaaaga
     8041 tctccttaaa catggatttc acgcatcaac cgagcgggtt agtgtgggcg aatacgctgg
     8101 cctggcaaga agcgcgtaaa gctcgcatta tccttggtaa gacaaatgcg caatacatca
     8161 gcgaatattc agattacaag cagtatgttg acgaaaaact ggatagcagc ctgacctggg
     8221 acacccgctt gtcctggacg ccacaatttc tgaaacaaca aaacctgacg atcagtgccg
     8281 atattctcaa tgtactggat agcaaaaccg ctgttgatac aacgaacacc ggtgtggcga
     8341 cctacgccag tggccgtact ttctggcttg atgtcagcat gaaattttaa tagttcggat
     8401 tttacttaac cgggtaaatt cgcccggttt tcgcatggag ataattatga gaaacctctg
     8461 tttcttactg acgttagtgg caactctgtt gctccccggg cggctgattg ccgccgcctt
     8521 accgcaggat gaaaagttaa ttaccgggca actggacaat ggcttgcgat atatgattta
     8581 tccgcatgct catccaaagg atcaggtaaa tttatggctg caaattcata ccggttcatt
     8641 gcaggaagaa gacaatgagc gcggcgtggc tcattttgta gaacatatga tgtttaacgg
     8701 cacaaaaaca tggccgggta ataaagtcat cgaaacattt gagtcaatgg gcctgcgttt
     8761 tggtcgcgat gttaatgcct ataccagcta tgacgaaacg gtgtatcagg tgagtttgcc
     8821 gactacgcag aaacaaaatc tgcaacaagt gatggcaatc ttcagtgaat ggagtaatgc
     8881 cgccaccttt gaaaaactcg aagtagacgc tgaacgtggc gtaattactg aggaatggcg
     8941 tgcccatcag gatgcgaaat ggcgcacctc tcaggcgcgc cgccctttcc tgctggctaa
     9001 tacccgtaat ttagaccgtg aacctatcgg cctgatggat actgtcgcca cggtcacacc
     9061 ggcacaattg cgccaatttt atcaacgctg gtatcaaccc aataatatga cctttatcgt
     9121 ggtcggcgat atcgacagta aagaagcgct ggcgctgata aaggataatt taagtaagct
     9181 tccggctaac aaagcagctg aaaatcgcgt ctggccgaca aaagctgaaa atcacctgcg
     9241 ctttaatatc attaatgata aagaaaaccg ggtgaacggc atcgcactct attatcgcct
     9301 gccaatggta caagtgaacg atgagcaaag ctttatcgaa caagctgaat ggagcatgtt
     9361 agttcagctg ttcaatcaac gtctgcagga acgcatacag tcgggcgagt tgaagactat
     9421 ttctggcggc actgcgcgca gcgttaaaat tgcacccgat tatcagtcgc tgtttttccg
     9481 tgtaaatgca cgagacgata atatgcagga tgctgcgaat gcattaatgg cagagttggc
     9541 aaccattgat cagcatggtt tttctgctga agaactcgat gatgtcaaat ctacccgcct
     9601 cacctggctg aaaaatgcgg ttgatcagca agctgaacgt gatttacgta tgctgaccag
     9661 tcgcctggca tccagctcat taaataatac gccgttcttg tcgccggaag agacatatca
     9721 actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt ctggcggaaa aatggcagca
     9781 gttaagaaag aaccaggacg cattttggga gcaaatggta aacaatgagg ttgccgccaa
     9841 aaaagcattg tctcctgcgg ctatcctggc gctggaaaag gagtacgcca acaaaaagct
     9901 ggcggcttac gtcttcccag gcagaaattt atcgttaaca gtagacgcag acccacaggc
     9961 ggaaattagc agcaaagaaa cgctggcgga gaatctgaca tcattaacac tttccaatgg
    10021 tgccagggtt attctggcaa aatccgcggg tgaagagcaa aagctacaaa ttattgccgt
    10081 atcgaataaa ggcgatttaa gtttccctgc gcagcaaaaa tcacttatcg cgctggcaaa
    10141 taaagcagtt agcggaagcg gcgttggcga actctcctct tccagcctga aacgctggag
    10201 tgcggaaaat tcggtaacca tgagcagtaa agtcagtggc atgaatacgt tgctctccgt
    10261 tagcgcgcgg actaataacc ctgaacctgg ttttcagttg attaaccagc gaatcaccca
    10321 cagcacgatt aacgataata tttgggcatc gctacaaaat gctcaaattc aggcgttgaa
    10381 aacgctcgac cagcgtccag cggagaaatt cgcccagcag atgtatgaga cgcgctatgc
    10441 tgatgaccgc acgaaattac tgcaagaaaa tcagattgca cagtttactg ccgcagatgc
    10501 gctggctgcc gatcgccaat tgttttcatc tccagcggat atcacgtttg tcattgtcgg
    10561 taatgtcgca gaagacaaac tcgtggcgtt aattacgcgt tacttaggat caatcaaaca
    10621 ctctgattcg ccattagccg caggtaaacc attaactcgc gcgacggaca acgcatcggt
    10681 tactgtaaaa gaacaaaatg aacctgtggc acaggtttca cagtggaagc gttatgattc
    10741 ccggacacct gttaatctgc cgacgcatat ggcgctcgat gcttttaacg tcgcactggc
    10801 aaaagatcta cgcgttaata ttcgtgaaca ggcatctggg gcatacagcg tttcttctcg
    10861 cctctcggtt gatcctcagg ccaaagatat cagtcatttg ctggctttta cttgtcaacc
    10921 agaacgacat gatgaactgt taacgttagc gaatgaagtg atggttaagc gtctggctaa
    10981 agggatcagt gagcaagaac tgaatgaata ccagcaaaac gttcagcgca gcctcgatat
    11041 ccaacagcgt agcgttcaac aattagcgaa caccattgta aatagtctta ttcaatatga
    11101 cgatcctgca gcatggactg agcaggagca attgttgaaa caaatgacgg tagagaatgt
    11161 taacactgcc gttaaacaat atctttctca tccggtaaat acttataccg gagtattatt
    11221 gccaaaataa taacagcccc gtcaacacat cgttggcggg gattttagca atattcgcta
    11281 tttttatgta ataattttat aaatgcgttc aaaataataa tcaagtacta atagtgatat
    11341 tttaaggtct gatttttacg tgataattca ggagacacag aatgcgcata aaaataacag
    11401 cataaaacac cttaccacca cccaagaatt tcatattgca ttgtttttca atgaaaaaat
    11461 attattcgcg taatatctca cgataaataa cattaggatt ttgttattta aacacgagtc
    11521 ctttgcactt gcttacttta tcgataaatc ctactttttt aatgcgatcc aatcatttta
    11581 aggagtttaa aatggataag aagcaagtaa cggatttaag gtcggaacta ctcgattcac
    11641 gttttggtgc gaagtctatt tccactatcg cagaatcaaa acgttttccg ctgcacgaaa
    11701 tgcgcgacga tgtcgcattc cagattatca atgacgaatt atatcttgat ggcaacgctc
    11761 gtcagaacct ggccactttc tgccagacct gggacgacga aaatgtccac aaattgatgg
    11821 atttatccat taa
//
LOCUS       DAZFZC010000130        11767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFZC010000130 DAZFZC010000000
VERSION     DAZFZC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.131"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..139
                     /locus_tag="ACHKXP_004337"
     CDS             <1..139
                     /locus_tag="ACHKXP_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7649215.1"
                     /translation="NIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWP
                     F"
     gene            463..1851
                     /gene="narU"
                     /locus_tag="ACHKXP_004338"
     CDS             463..1851
                     /gene="narU"
                     /locus_tag="ACHKXP_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO7649216.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1933..5673
                     /gene="narZ"
                     /locus_tag="ACHKXP_004339"
     CDS             1933..5673
                     /gene="narZ"
                     /locus_tag="ACHKXP_004339"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO7649217.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5670..7214
                     /gene="narH"
                     /locus_tag="ACHKXP_004340"
     CDS             5670..7214
                     /gene="narH"
                     /locus_tag="ACHKXP_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7649218.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKQMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7214..7909
                     /gene="narW"
                     /locus_tag="ACHKXP_004341"
     CDS             7214..7909
                     /gene="narW"
                     /locus_tag="ACHKXP_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7649219.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTSQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7906..8586
                     /gene="narI"
                     /locus_tag="ACHKXP_004342"
     CDS             7906..8586
                     /gene="narI"
                     /locus_tag="ACHKXP_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7649220.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNDVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8665..9558
                     /gene="yddE"
                     /locus_tag="ACHKXP_004343"
     CDS             8665..9558
                     /gene="yddE"
                     /locus_tag="ACHKXP_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO7649221.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMLPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGNQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9654..10499)
                     /gene="nhoA"
                     /locus_tag="ACHKXP_004344"
     CDS             complement(9654..10499)
                     /gene="nhoA"
                     /locus_tag="ACHKXP_004344"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO7649222.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDNQSPEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLMELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10672..11241
                     /gene="yddH"
                     /locus_tag="ACHKXP_004345"
                     /pseudo
     CDS             10672..11241
                     /gene="yddH"
                     /locus_tag="ACHKXP_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(11238..11471)
                     /gene="pptA"
                     /locus_tag="ACHKXP_004346"
     CDS             complement(11238..11471)
                     /gene="pptA"
                     /locus_tag="ACHKXP_004346"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO7649223.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSMNA"
     gene            complement(11571..>11767)
                     /locus_tag="ACHKXP_004347"
     CDS             complement(11571..>11767)
                     /locus_tag="ACHKXP_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7649224.1"
                     /translation="VVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLTGSGLS"
BASE COUNT         2779 a         3041 c         3081 g         2866 t
ORIGIN      
        1 gaatataaaa gttaaaaaca tgaccttaga tgaaataaac aatacttact gtaataacga
       61 ttattacaat caggcaataa gagaggaacc gatagacctt ctggacagat cgttttcctc
      121 cagttcatgg cctttttagt gaataaatca tcactaattc tataagtgtc tggtcttgtt
      181 gataatatag ttaatatgta accctttaga ttcacgatct gaagggttga aacatgacat
      241 acgttcaaag attaaccccg tttgaaggtt agcttgttag ttacatttag taacacatat
      301 ctggctactt aatccccatt ctgcccccgc ggttacgctt tcttatctat tctttcaatc
      361 agcacttcac ccaattcatc cttaattcta ataactggct attgcgcctt acctcgtgtt
      421 ttgtcttttt ttcattacca atgtgtgcat gtgaggaaca atatggcact gcaaaatgag
      481 aaaaatagtc gttatctttt gcgcgactgg aaaccagaaa atccggcctt ctgggaaaat
      541 aaaggaaaac atattgctcg aagaaatctc tggatatcag tcagttgtct acttcttgcc
      601 ttctgtgtct ggatgctatt tagcgcagtt accgttaatc tcaataaaat cggttttaat
      661 tttactaccg atcaactctt tttattaacc gcattaccct ccgtttctgg cgcattattg
      721 cgtgttccct actcctttat ggtgcctata ttcggtggac gccgatggac ggtttttagt
      781 actgcaatcc tgattattcc ttgcgtctgg ctcggaattg ccgtgcaaaa tccgaatact
      841 ccttttggga tatttatcgt tatcgctttg ctatgcggtt ttgcaggtgc aaactttgct
      901 tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca aacaagggag cgctcttggg
      961 attaatggcg gattaggaaa cttaggtgta agtgtaatgc agctggttgc accgctggtc
     1021 atttttgtac ctgtatttgc ctttctcggc gtcaatggcg taccgcaggc cgacggttcg
     1081 gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc tactggcgat tgccacgatc
     1141 gccgcatggt cagggatgaa tgatatcgcc agttcacgcg cctcaattgc cgaccagctc
     1201 cctgtcttac aacgcctgca tctctggctg ctgagcctgc tttaccttgc caccttcggt
     1261 tcgtttatcg gtttttctgc gggttttgcc atgctggcaa aaacccagtt cccggatgtg
     1321 aatattctgc gcctggcgtt ctttggccca tttatcggtg ccatcgcgcg gtcggttggt
     1381 ggtgctattt ccgataagtt cggcggcgtg cgggtgacgt tgatcaactt tatttttatg
     1441 gcgattttca gtgccctgct gttccttacc ttaccgggca caggctccgg taatttcatc
     1501 gccttttacg ccgtatttat ggggctgttt ctgactgcgg gtctgggaag tggttctact
     1561 ttccagatga tcgccgtcat ctttcgccag ataaccattt atcgggtaaa gatgaaaggc
     1621 ggtagtgatg agcaagctca gaaagaagcc gtcaccgaaa cggcggcggc tctgggcttt
     1681 atctcagcca ttggcgcagt gggcggcttt tttattccgc aggcgtttgg catgtcgctc
     1741 aatatgaccg gctctccggt cggcgcgatg aaagtgtttt taatcttcta catcgtttgt
     1801 gtgctgctga cctggctggt ttatggtcgg cggaagttca gccaaaaata agagatcgat
     1861 gcctgatgta caaccgtgtc gcatccgaca aggcatcaga tcccaattca ttcctggagc
     1921 aggagtcatg tcatgagtaa acttttggat cgctttcgct acttcaaaca aaagggcgaa
     1981 acctttgccg atggtcacgg acaggtgatg catagcaacc gcgactggga ggacagctat
     2041 cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc acggtgttaa ctgtacaggc
     2101 tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga cctgggaaat ccaacagacc
     2161 gactacccgc gcactcgccc tgacctgccc aatcatgaac ctcgcggctg cccgcgtggc
     2221 gcaagttact cctggtatct ttacagcgct aaccgcctga aatacccgct cattcgtaaa
     2281 cgactgattg aactgtggcg cgaagccctc aagcaacaca gcgatccggt actggcgtgg
     2341 gcatcgatta tgaacgatcc gcaaaagtgc ctgagctaca aacaagtgcg tgggcgcggc
     2401 gggtttatcc gctccaactg gcaggaacta aaccagctga ttgccgccgc taacgtctgg
     2461 accatcaaaa cctacggccc ggatcgcgtt gccggtttct cgccgatccc ggcgatgtcg
     2521 atggtttctt acgccgccgg aacgcgttat ctgtcgctgc ttggcggcac ctgtttaagt
     2581 ttctacgact ggtattgcga cctgccgccc gcctcgccga tgacctgggg cgagcaaacc
     2641 gacgtaccgg aatctgccga ctggtataac tccagctaca tcatcgcctg ggggtctaac
     2701 gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg aagtacgcta caaaggcact
     2761 aaaaccatcg ccattacccc tgactactct gaagtggcca aattgtgcga ccagtggctg
     2821 gcaccgaaac aaggcactga tagcgccctg gcgatggcaa tgggccatgt gattttaaaa
     2881 gagtttcatc tcgataatcc cagcgactac tttatcaact actgccgccg ctacagcgac
     2941 atgccgatgc tggtaatgct ggagcctcgc gacgatggta gctacgttcc cgggcgcatg
     3001 atccgcgcat ctgacctggt ggatggactg ggcgaaagca acaatccgca gtggaaaacc
     3061 gtagcagtta ataccgcagg tgaattggta gtgccgaacg gttcgattgg tttccgctgg
     3121 ggagaaaaag gcaaatggaa tctggaatcc attgccgccg gtacggaaac cgaattgtcg
     3181 ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg ccttccccta ctttggcggc
     3241 attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg tgctggtgcg ccaattgccc
     3301 gttaaaaacc tgacgttagt cgatggcaac acctgtccgg tggtcagcgt ttatgatttg
     3361 gtactggcga attacggcct cgatcgcggg ctggaagatg aaaacagtgc gaaagattac
     3421 gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa ttaccggcgt gccgcgccag
     3481 tatattgaaa ccatcgcccg tgaatttgcc gataccgccc ataaaacgca tgggcgctcg
     3541 atgattatcc tcggcgcagg tgttaaccac tggtatcaca tggacatgaa ctaccgcggg
     3601 atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa gcggtggcgg ctgggcacac
     3661 tatgtcggcc aggaaaaact gcgcccacaa accggctggt tgccactggc ctttgcgctc
     3721 gactggaacc gaccaccgcg ccaaatgaac agcacctcgt ttttctacaa tcattccagc
     3781 caatggcgct atgaaaaagt ctctgctcag gagttacttt caccgctcgc cgatgccagt
     3841 aagtacagcg gtcatctgat tgatttcaac gttcgcgccg aacgtatggg ctggctacct
     3901 tctgcgccgc agctggggcg taacccgctc gggattaaag ctgaagccga caaggccgga
     3961 ttatccccca cagaatttac cgcccaggcg ctgaaatcgg gcgatttacg tatggcctgc
     4021 gaacaaccag atagcagcag caatcatccg cgtaatttgt ttgtctggcg ctctaacctg
     4081 cttggctcct ccggcaaagg ccacgagtat atgcagaagt atctgctggg gaccgaaagc
     4141 gggattcagg gcgaggaact cggtgccagc gacgggatca aaccggaaga agtcgagtgg
     4201 caaactgcag cgattgaagg caagctcgac ctgctggtga cgctcgactt ccgcatgtcc
     4261 agtacctgcc tgttctccga tatcgttctg cccaccgcca cctggtacga aaaagacgat
     4321 atgaacacct cggatatgca tccgtttatt catccgcttt ctgcggcggt cgatccggcg
     4381 tgggaatcac gcagcgactg ggaaatctac aaaggtatcg ccaaagcatt ttcgcaagtg
     4441 tgcgtgggtc atcttggcaa agaaaccgac gtggtattac aacccctact gcatgactct
     4501 ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc gcaaaggcga atgcgatctg
     4561 attccaggca aaaccgcgcc gaatattgtg gcggtggagc gcgactaccc tgctacgtat
     4621 gaacgcttta cctcgctcgg gccattgatg gacaaacttg gcaatggcgg taaagggatt
     4681 tcgtggaata cccaggatga aatcgatttc ctcggcaaac tcaattacac caagcgtgat
     4741 ggcccggcac aggggcgtcc gctgattgac accgccattg acgcttcaga agtgattctg
     4801 gcactggcac cggaaaccaa cggtcatgtt gcagtcaaag cgtggcaggc gctgggcgag
     4861 atcaccgggc gcgaacatac ccatctggcg ctgcacaaag aggacgagaa gattcgcttt
     4921 cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc ccacctggtc cggtctggaa
     4981 agcgatcacg tctcctataa cgcgggatac accaacgttc atgagttaat tccgtggcgc
     5041 acgctgtcgg gacgccagca gctctatcag gatcatccgt ggatgcgtgc ttttggtgaa
     5101 agcctggtgg cttatcgccc gcctatcgac acccgtagcg tcagtgagat gcgccagata
     5161 ccgccaaacg gcttcccgga aaaagcactt aacttcctga cgccgcacca gaaatggggc
     5221 attcactcaa cctacagtga aaacctgcta atgctgacgc tctctcgcgg tggaccgatt
     5281 gtctggatca gcgaaacaga tgcccgagaa ctgaccattg tcgataacga ctgggtggaa
     5341 gtattcaacg ccaacggcgc gctgactgcc cgcgcggtgg tcagccaacg tgtaccgccg
     5401 ggcatgacca tgatgtatca cgcccaggaa cgcattatga atattcctgg ttcggaagta
     5461 actggcatgc gcggcggcat tcataactcg gttacccgcg tttgcccgaa accaacgcat
     5521 atgattggcg gttacgcgca gctggcctgg ggctttaact actacggcac cgtcggatcg
     5581 aaccgcgatg agttcatcat gatccgcaag atgaagaacg ttaactggct ggatgatgaa
     5641 ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatccg ttcacaagtc ggcatggtgc
     5701 ttaacctcga caaatgtatc ggctgccata cctgttcggt gacctgtaaa aacgtctgga
     5761 ccgggcgcga aggcatggag tacgcatggt ttaacaacgt cgaaaccaaa ccgggcattg
     5821 gttatccgaa aaactgggaa gatcaggaag agtggcaagg cggctgggtc cgcgatgtga
     5881 atggcaagat ccgtccgcgt ctgggcagca agatgggcgt aataaccaaa atcttcgcca
     5941 acccggtggt gccgcagatt gatgattact acgaaccttt cactttcgac tacgaacatt
     6001 tgcatagcgc accggaaggc aaacatatcc ctactgctcg cccgcgctcg ctgattgacg
     6061 gtaagcagat ggacaaagtg atctgggggc caaactggga agaactgctg ggcggcgagt
     6121 ttgaaaaacg tgcccgcgac cgcaacttcg aggccataca aaaggagatg tacgggcagt
     6181 ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga acactgcctc aatcccagtt
     6241 gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga agaagacggc attgtgctga
     6301 ttgatcagga taaatgccgt ggctggcgtt tgtgcattag cggttgtccg tacaaaaaaa
     6361 tctacttcaa ctggaaaagc ggcaagtcag aaaaatgtat cttctgttat ccgcgaattg
     6421 agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg tcgcatccgg tatctgggcg
     6481 tgctgcttta cgacgccgac cgcatcgagg aagcggcgag caccgagcgc gaagttgacc
     6541 tctatgaacg tcagtgcgaa gtgttcctcg atccacacga tccctcagtg atcgaggaag
     6601 ccctgaaaca aggtattcca caaaacgtga ttgaagctgc ccagcgttcg cctgtctaca
     6661 aaatggcgat ggactggaaa ctggcgctac cgctgcaccc tgaatatcgc accctgccga
     6721 tggtctggta cgttcctccg ctgtcgccga ttcagtccta cgcagatgcg ggcggtttgc
     6781 cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg tattccggtg caatatctcg
     6841 ccaatatgtt gagtgccggc gataccggtc cggtactgcg ggcgctgaaa cggatgatgg
     6901 cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt tactgatact cgtgccatcg
     6961 acgaagtagg cctgagcgtc gcccaggtcg aagaaatgta tcgctatctc gccattgcca
     7021 actatgaaga tcgttttgtt atcccgacca gccatcggga aatggcgggc gatgccttcg
     7081 cagaacgcaa cggctgcggt tttacctttg gcgacggttg tcacggctcg gacagcaaat
     7141 tcaacctgtt caacagtagc cgtatcgatg ccatcaacat caccgaagtg cgcgacaaag
     7201 cggagggcga ataatgcaga tcctcaaagt gatcggcctg ttgatggagt atccggatga
     7261 gctgttgtgg gaatgcaagg aggacgcgct ggcgttgatc cgccgcgacg cgccgatgct
     7321 tacggatttc acccacaacc tgcttaacgc gccgctgctg gataaacagg ccgaatggtg
     7381 cgaagtgttt gaccgtggac gtaccacatc gctgctgctg ttcgaacatg ttcatgccga
     7441 gtcccgcgat cgcggccagg caatggtgga cctgctggcg gagtatgaaa aggtcggcct
     7501 gcaactggat tgtcgggaac tgcccgatta tctaccgctg tatctggagt atttaagcgt
     7561 actgcctgac gatcaggcga aagaaggatt gctcaatgtt gcgccgatcc tcgccctgct
     7621 tggcggtcgc ttaaaacaac gcgaggcacc gtggtacgcg ttgtttgatg ctctgctgca
     7681 actggcggga agctcccttt caagtgacag tgtcacaaaa caagtaaaca gcgaagagcg
     7741 cgatgacacc agccaggcgc ttgatgcagt gtgggaagag gaacaggtga agtttattga
     7801 agataacgcc acggcgtgtg acagctcgcc gcttaatcaa tatcagcgac gctttagcca
     7861 ggatgtcgcg ccgcaatatg tcgacatcag tgcgggaggt gggaaatgat tcagtatctg
     7921 aacgtctttt tttacgatat ctacccgtac atttgtgcga cggtgttttt cctcggcagc
     7981 tggctgcgct acgactacgg gcagtacacc tggcgcgcct cctcaagtca aatgctcgat
     8041 aaacgcggga tggtgatatg gtcgaatctg ttccatatcg gcattttggg gattttcttc
     8101 gggcacttgt tcggcatgtt aacgccgcac tggatgtacg cgtggttttt gccagtggca
     8161 gcgaaacaac tcatggcgat ggtgctcggc ggtatttgcg gtgtgttgac gttgattggc
     8221 ggcgcaggac tgctgtggcg caggctgaca aatcagcgcg tgcgtgccac ttccactacg
     8281 ccggatatca tcatcatgag cattttgctg gtccagtgtc tgttggggct aagtaccata
     8341 ccgttttcag cacagtatcc tgatggtagc gaaatgatga agctggtggg ttgggcgcaa
     8401 agcattgtca ctttccgtgg tggatcgtca gagatgctca acgacgtagc gttcgtcttc
     8461 cgcctgcatc tggtgttggg aatgaccatc ttcctgctct tcccgttcac ccgactggtg
     8521 catgtgtgga gcgcgccgtt tgagtacttt actcgtcgat atcaaattgt gcgttcgcgg
     8581 cgataatttc accctggccg gatagccctt cgctctccgg ccacttgcag gctatgatag
     8641 ttctccagtc acaggagaac gttaatgaaa ccgcaggtgt accacgtcga tgcttttacc
     8701 tcacaaccgt ttcgcggcaa ttctgccggt gttgttttcc ccgccgataa tcttagcgaa
     8761 gcgcaaatgc agcttatcgc ccgcgagtta ggccattcgg aaactgcttt tctgctgcac
     8821 agcgacgaca gcgatgtgcg catccgctac tttacgccaa cggttgaagt gccgatttgc
     8881 ggtcacgcga cagtagctgc gcactatgta cgtgcgaagg tgttaggttt aggaaattgc
     8941 acgatctggc aaacatcgct ggcaggaaaa catcgcgtga ctatcgaaaa gcacaacgat
     9001 gattatcgta tttcgctgga acaaggtacg ccgggctttg aactgccgct ggagggtgaa
     9061 gtacgcgcgg cgattatcaa cgcattgcat cttactgaag acgatattct gccgggtttg
     9121 ccgatccagg tcgcgacgac cgggcattct aaagtgatgc tcccattgaa gccggaagtg
     9181 gatatcgacg ctctttcgcc tgaccttaat gcgctgaccg ctatcagtaa acagattggc
     9241 tgcaatggtt tcttcccgtt ccagatccgt ccaggcaaaa acgaaaccga tggtcgtatg
     9301 ttctcgcctg cgattggtat tgtggaagat ccggtgactg gcaatgccaa tggcccgatg
     9361 ggtgcatggt tggtacatca caacgtattg ccccacgatg gcaaggtgct gcgtgttaaa
     9421 ggcaatcagg ggcgcgcatt ggggcgtgac ggcgtgattg aagtgacggt gacaattcgt
     9481 gataaccaac cggaaaaagt caccatttct ggcgcggcgg tgattttgtt tcatgccgaa
     9541 tgggcaatta aactctgaaa ccgttgcctg atgcgcttcg cttatcaggt ctacaaccgg
     9601 gttaatgtat gccggataag gcgtttacgc cgcatccggc aacctgacat aaattatttt
     9661 cccgcctccg ggtgcgtatc aaacgccgcc atcaccagcg ctaactcatc cacggtaaag
     9721 ccatgtttcg catcatccac gcccagacca aactgttctt gcatcacagc atataatgac
     9781 gccacatccg gtagatttcg ctgctccacc gcgtgcccat tttcataatg ggtaaaatga
     9841 aaattggtca gtgtcagctt gccgccgtcc ggcaaatggc ggcacatcag caaatgatgg
     9901 cgaaaatgcg actgcggcca gtgcgccgac cagaaattgc ccatcacata atcgctttgt
     9961 tgctgctcgc agagatcaaa acggtacatc gactgccaat gctgatgatg attaaactgc
    10021 aacacccaat catcaccctc ctgcaacaac cgatactctc cgtgtggcgt ggtctgcacg
    10081 agatcggaaa ctaaacgaat cggcgcggtt agcgtctgcc caccgaaacc gacatcagca
    10141 atccattttt cctcttccag ttccatcaac agcaaacgat gggtgcgcgg cggtaatgct
    10201 ggcggatttg ataacactac gcgccctaac aagctgcgaa cgttaaaccc cagctcgcgt
    10261 aacacccgct caaacacgcc attctgctca aaacagtaac cgccacgacg ggctatcacc
    10321 agtttctctt ccggcgattg attatcaagc tgtatttccc tcggcagcaa aacgtcgagg
    10381 ttttcaaacg gaatggtgca attgtgtttc aggtgcaatg cacgaagcgt atcaatattg
    10441 accgcagcag ctcccgacca gttaatacgg gcaaaatagt gattcagaat gggcgtcatc
    10501 aggcgtttcc cttagtggtt ttctcaactt ataacctgga atagacgccg caccggctat
    10561 tttgtgcgat cgcggacatg catcacgttc gcaaatcgcc aatcccgtta atttcgttac
    10621 ttattgcgtt acggactacg cttaaaaaaa ccttcttcgc gaggcgatac catgagccga
    10681 ttcatcccca tagaattaca ccatgccagc cgtctgttaa atcatggccc aacggtcttg
    10741 atcaccagtt tcgatgagca atcccagcgg cgtaacatta tggctgcagc ctggtcaatg
    10801 ccggtggagt ttgaacctcc acgcgtggcg attgtggtag ataaatcgac atggaccagg
    10861 gagttgattg aacataacgg taaatttggc atcgttatcc cgggcgttgc agcaactaac
    10921 tggacgtggg cggtgtgaag tgtgtcgggg cgtgatgaag ataaatttaa ttgctatggc
    10981 attccggttg tgagaggccc ggtatttggt ttgcctctgg tcgaggaaaa atgtctggcg
    11041 tggatggagt gtcgattgct acctgcgact tctgcgcaag aagaatacga cacgctgttt
    11101 ggcgaagtag tatcagcagc ggcagacgca cgggtatttg tcgaaggccg ctggcagttt
    11161 gatgatgata agctcaatac gttgcatcat ttaggtgctg ggacgtttgt taccagcggc
    11221 aagcgtgtta cggcgggtta agcattcatg ctataaccag gtttctttat caaagcctcc
    11281 atttggggcg cgatttcggc atcccagata gcttgccaag attctggttg aatctgctgt
    11341 agagcaatgc ttatcgaact gtctttactg ttcagatgac gaataataac gtcggtaata
    11401 tctgcagcaa gtgctgcttt ttgttgttcg tccagttcac gcggaaaaca tttaatgtcg
    11461 atgtgcggca tgattacgct ccttttgaaa gatgtcagta aaattaacac acattgtgaa
    11521 tgtcatgtgt ccggtaaagc gtttatacga caataatgcc cagggcaaga ttacgaaagc
    11581 ccgctccccg taaggactga cgccaggtag tttctgtcca tggctgcttt tcgcatctta
    11641 cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa tattaatagc aatgtgccgt
    11701 atccctgaaa ataattctgc tgcatttcct cttcttattt tgcagtcgtc ttcattcatt
    11761 accacgt
//
LOCUS       DAZFZC010000131        11523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFZC010000131 DAZFZC010000000
VERSION     DAZFZC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.132"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..946)
                     /locus_tag="ACHKXP_004348"
     CDS             complement(<1..946)
                     /locus_tag="ACHKXP_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7649225.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPL"
     gene            complement(1377..3791)
                     /gene="ybbP"
                     /locus_tag="ACHKXP_004349"
     CDS             complement(1377..3791)
                     /gene="ybbP"
                     /locus_tag="ACHKXP_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO7649226.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(3788..4474)
                     /gene="ybbA"
                     /locus_tag="ACHKXP_004350"
     CDS             complement(3788..4474)
                     /gene="ybbA"
                     /locus_tag="ACHKXP_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO7649227.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            4445..5068
                     /gene="tesA"
                     /locus_tag="ACHKXP_004351"
     CDS             4445..5068
                     /gene="tesA"
                     /locus_tag="ACHKXP_004351"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO7649228.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5058..5867
                     /gene="ybbO"
                     /locus_tag="ACHKXP_004352"
     CDS             5058..5867
                     /gene="ybbO"
                     /locus_tag="ACHKXP_004352"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7649229.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            5928..6782
                     /gene="cnoX"
                     /locus_tag="ACHKXP_004353"
     CDS             5928..6782
                     /gene="cnoX"
                     /locus_tag="ACHKXP_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO7649230.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQESNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(6845..7624)
                     /gene="fetB"
                     /locus_tag="ACHKXP_004354"
     CDS             complement(6845..7624)
                     /gene="fetB"
                     /locus_tag="ACHKXP_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO7649231.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(7611..8288)
                     /gene="fetA"
                     /locus_tag="ACHKXP_004355"
     CDS             complement(7611..8288)
                     /gene="fetA"
                     /locus_tag="ACHKXP_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO7649232.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            8434..9351
                     /gene="qmcA"
                     /locus_tag="ACHKXP_004356"
     CDS             8434..9351
                     /gene="qmcA"
                     /locus_tag="ACHKXP_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7649233.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            9348..9806
                     /gene="ybbJ"
                     /locus_tag="ACHKXP_004357"
     CDS             9348..9806
                     /gene="ybbJ"
                     /locus_tag="ACHKXP_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO7649234.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(9807..10214)
                     /gene="cueR"
                     /locus_tag="ACHKXP_004358"
     CDS             complement(9807..10214)
                     /gene="cueR"
                     /locus_tag="ACHKXP_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO7649235.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10211..10308)
                     /locus_tag="ACHKXP_004359"
                     /pseudo
     CDS             complement(10211..10308)
                     /locus_tag="ACHKXP_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10339..>11523)
                     /gene="ybaT"
                     /locus_tag="ACHKXP_004360"
     CDS             complement(10339..>11523)
                     /gene="ybaT"
                     /locus_tag="ACHKXP_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7649236.1"
                     /translation="QAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDF
                     FRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGI
                     IAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFF
                     SCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSA
                     LELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERE
                     LPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRL
                     RHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNK
                     TV"
BASE COUNT         2787 a         3253 c         2973 g         2510 t
ORIGIN      
        1 ccagtggcgc agcgggcagg ctctccgggt atgccgggtc gtgcatcagc cacaccgccg
       61 aaaggcggat acccctgtcg gggccgtatt cggtaccggg cagtgtgtcg gggaacgctg
      121 aggctgagag agggcgggaa ctgtcagaag aagatagcga agaggtgcgg gcctcttccg
      181 cacgctgcgc ctgcgtggtc agcaccagac ggaactcccg cccggcaccg tccgtcacgc
      241 cggtgatttc cccggccagg tcaccggcgg cctcacgccg gtacgtcagc gtccgcccga
      301 agcggtccgc catcccggta agcacccggt acggcggcag cggcgctggc agtacgtcct
      361 cagcacccgg cacccgctca gaccacccca gtatccacca cggcccctgt gcgctgttgg
      421 tcgccaggta aagatgcggg cttaaccgga tatccggcgg cagcgccccc cacagccgcg
      481 ccagcgtatg gccgtccggc tgtgctgcct taccaccgcg caccagccac attgactcac
      541 tgcggctgta caccgcctcc cccggcagca gcggctcaaa gtgaatgctc cgcccgccgt
      601 tgtcgttgag tatcagtccg tcatcacgta gctgtaagcg gatatcagaa ggcgctttcc
      661 agccggggcc gaaaacgccc accggtgcag gcgtcttcgt ccggtagctg ctgtaggtgc
      721 gggagagaat gaacggcagc gggccgggca gcgcaaggtc cgtctcgccg ggcagcacct
      781 tcgcccccag cagcggattt accgggttgc ccgaagtcat cccgcccgga cacaccgagc
      841 acgccacgcc ggtgggcgcg ccaattctta cacctgccga accctggaca atgggaccgc
      901 catactgagt catatctccc tgacgcgccg ctggttttcc gctcatataa agtgcccttt
      961 tagttattgt tcatatccct gtgttagcaa caaaaaaaaa taaaatgaca cacaagacca
     1021 gctaatgtat ttatatattt ttcacataaa cttatttgtt ctcaatcaac aacaactgtt
     1081 attacaggtc actttcaata ttttattaaa ctaacatttt tagcacagat gaattcatca
     1141 tcaacaaatt cactatatgg ataatatcca ttcaacgcat atgatttatg attttattag
     1201 tctgagttat ttttgtaggg ctatatttaa aatcaataaa ctccaccgat tcagattaat
     1261 atcagcaata gaaagatagg aaatcaccaa ttcaataaca tcatcaatga aacatattgt
     1321 agaattaatt taaacaaggc gttccattca accagaacgc cccttattta ctctcatcac
     1381 cccgcaaact gcctgaacag cgccttaccc ttaaccagtc gcgcacccag ccagccgccg
     1441 aaaagcgaca gcagcagcgc tccgctgcac ggcagaacaa tccacaatcg ccagtctggc
     1501 tcccacggaa aatcaaacac tttcgcctgc aacactgcca gcgccgtttc cgcaccaatt
     1561 gcggccacca ggccggaaac aaacccaagc atggcgaact cacaccacaa cgtggtacgc
     1621 agcagttttt tccccgcacc gagtgtgcgc cacaccacca gctcctgatg acgctgacgc
     1681 attcccacct gcacctgtgc cagcaacagc aacataccgc aggcggtgac cagtaccacc
     1741 atcacttcca gcgcccgact tacctgctcc agcacctgac cgacctgttt taaaatcgcg
     1801 ccaatatcta acaggctaat ggtcgggaac tggcggttga gttgtgtcaa catgccgttg
     1861 ccattctccc agcggaaact ggtaagccag ctctgcggtt gcccgtctaa tgccccttca
     1921 gggaaaataa aatagaaatt aggccgcaga ctttcccagt ccactttgcg caggctggtc
     1981 actttagcgc ggaactcctg ggtatcgccc ataaaagtca cggtatcacc gagggcaacg
     2041 tttaagcgtt tcgccagccc ctcttccatc gacacttcat cggcttttgg cggccagtta
     2101 ccggcgacaa tcggattatg atcgggccgc gtattttgcc aggtaagatt gagttcgcgg
     2161 ttaagcgcct catcttcatt accttctgtc ggcttatcgt taatcgccgt cagccgcgcc
     2221 cgcaccaccg gataaaacga ttccgggact atctgatgtt ccgcgaggaa cgcttttagc
     2281 ggtgctacct gttctgtggc gatgttaatt aaaaagtagt tcgggctttc tggaggtagc
     2341 tgctgctgcc agcggtcgag cagatcgcca cgcaacacca gcagcagtgc cagcagcata
     2401 aaggagagcg aaaatgccga aagctgactt aacgttgacc acggctgacg taacaggcgg
     2461 ctaaccgcca ggcgcagagg cagcgatttc agcgtcatgc ggcgaagtac attcagcagc
     2521 atccagccca gcacaccgca cagcaaagcc agtactaccg cgcccgccag caccgcccaa
     2581 agcagcatgc tgccacccat taatccggcg agcagcagca caaccaccac actgacaatc
     2641 ggcagataaa acttcagcgg ccagacgttc gctaccacat cattacgtaa tacgcgtaaa
     2701 ggctgcgttg ccagcaacaa gcgatatggt cgtagcccca ccagcagcga gatgaccgtc
     2761 atggtgccaa gcgcccacag ccacggccag aggctggctg gcggtagtgc agcaggcaga
     2821 acgggcttga gcagcaccat caacacgttt tcgaacaaca ggcctatggc cccaccggta
     2881 acggctgaaa gcgtcagcac catcaaccac tgaccgacga ttagcttacg cagttgcgct
     2941 cgccctgccc ccagcgtttt gaggatcgcc accagatcgt agcgactgcg acagtaatga
     3001 ttcatcgcta ccgccaccgc tgccactgcc agcagcaagg tcagaagcgc cgaaagcagc
     3061 aggaactgtt gcgagcgttc catcgatcgc cccagcgcgc cttcgtcctg ttccagaccg
     3121 taccagcgtt gttcgggttt aagctgaggt aacaaccatt tctcatagcc gtcgagctgg
     3181 ttctcgttgc cgccgaattt ataacgccag gtgacccgac tccccggctg cacggctccg
     3241 gttttatcga catccgccag attcatcatc agacgcggag ccatctggaa ggggttaaaa
     3301 ccggaatccg gttcctgaat cacttctccg gcaatccgca aggtggcatc gcccacgtca
     3361 atggtgtcgc ccgttttcag gttaagcagt gccatcaggc gtggggccag caatacgctg
     3421 cccgcctgcg gtttcaggcc agggggatta gtttgcagat cgccatacat cgggtagata
     3481 tcatccaccg ctttgacgtt cgccagctgc ggtgtgtcgc ctgcaaaggt cattgtggcg
     3541 aaagtcagct gcttgccgac tttcaggccg cgcttttgcg cttcctccag ccacgcttgc
     3601 ggcacttcgc gtgaactgcg caacgcccga tcgcccgcca taaactcacg gctttgctgg
     3661 cttaagccct tctccatgcg atcgctgata ttgcccagcg ccagcacgca ggccaccgcc
     3721 aggcttagcg ccagccagac aattaatagc gacggcgaac gccattcgcg ccagaaccaa
     3781 cgtgcaatca tgcttcctcc tgcaactgcc cgttcaccag ccgtaagcag cggtcgcagc
     3841 gtgccgccag ttgcaggtcg tgggtcacca taatcaacgt ggtgccatgt tcacggttga
     3901 gggaaaacag caggtcggca attttatcgc ccgtctggcg gtcaaggttg ccggtgggtt
     3961 cgtcggcaaa cagcacatca ggtcgaccat taaaggctcg tgccagcgcc actcgttgct
     4021 gttcaccgcc ggaaagctgt gccggaagat gatccagacg tttacccagc cctaactgtt
     4081 cgagcaacgc tttcgccccg ttacgacttt ccgcgctact ctcaccgcgc agcagagccg
     4141 gaagctcgac gttttccagc gcgttaaggg taggaattaa cataaatgac tgaaaaacaa
     4201 agccgacgtg cttcgcgcgc aactttgccc gcgcttcttc gtccatatta tgtagcggtt
     4261 gtcccaccag actcacttcg ccactgctgc cgtcatcaag cccggcgagg atcgccagca
     4321 aggttgactt acccgatccc gactcgccca ccagtgcgat ggtctcgcca cgtttgacaa
     4381 ccagctcaac tccggtgagg atggagagtt catgctcccc ctgaccgacg gacttcttaa
     4441 gatgatgaac ttcaacaatg ttttccgctg gcatttgccc ttcctgttcc tggtcctgtt
     4501 aaccttccgt gccgccgcag cggacacgtt attgattctg ggtgatagcc tgagcgccgg
     4561 gtatcgaatg tctgccagcg cggcctggcc tgccttgttg aatgataagt ggcaaagtaa
     4621 aacgtcggta gttaatgcca gcatcagcgg cgacacctcg caacaaggac tggcgcgcct
     4681 tccggctctg ctgaaacagc atcagccgcg ttgggtgctg gttgaactgg gcggcaatga
     4741 cggtttgcgt ggttttcagc cacagcaaac cgagcaaacg ctgcgccaga ttttgcagga
     4801 tgtcaaagcc gccaacgctg aaccattgtt aatgcaaata cgtctgcctg caaactatgg
     4861 tcgccgttat aatgaagcct ttagcgccat ttaccccaaa ctcgccaaag agtttgatgt
     4921 tccgctgctg ccctttttta tggaagaggt ctacctcaag ccacaatgga tgcaggatga
     4981 cggtattcat cccaaccgcg acgcccagcc gtttattgcc gactggatgg cgaagcagtt
     5041 gcagccttta gtaaatcatg actcataaag caacggagat cctgacaggt aaagttatgc
     5101 aaaaatcggt cttaattacc ggatgttcca gtggaattgg cctggaaagc gcgctcgaat
     5161 taaaacgcca gggttttcat gtgctggcag gttgccggaa accggatgat gttgagcgca
     5221 tgaacagcat gggatttacc ggcgtgttga tcgatctgga ttcaccagaa agtgttgatc
     5281 gcgcagccga cgaggtgatc gccctgaccg ataattgtct gtatgggatc tttaacaatg
     5341 ccggattcgg catgtatggc cccctttcca ccatcagccg tgcgcagatg gaacagcagt
     5401 tttccgccaa ctttttcggc gcacaccagc tcaccatgcg cctgttaccc gcgatgttac
     5461 cgcacggtga agggcgtatt gtgatgacat catcggtgat gggattaatc tccacgccgg
     5521 gtcgtggcgc ttacgcggcc agtaaatatg cgctggaggc gtggtcagac gcactgcgca
     5581 tggagctgcg ccacagcgga attaaagtca gcctgatcga acccggtccc attcgtactc
     5641 gcttcaccga caacgtcaac cagacgcaaa gtgataaacc agtcgaaaat cccggcatcg
     5701 ccgcccgctt tacattggga ccggaagcgg tggtggacaa agtacgccat gcttttatta
     5761 gcgagaagcc gaagatgcgc tatccggtga cgctggtgac ctgggcggta atggtgctta
     5821 agcgcctgct gccggggcgc gtgatggaca aaatattgca ggggtgagtt gaagcgcgcg
     5881 cttaagcccc catgtcaaag aaaatatcga caacagagag cgactccatg tccgtagaaa
     5941 atattgtcaa cattaacgaa tctaacctgc aacaggttct tgaacagtcg atgaccactc
     6001 cggtgctgtt ctatttttgg tctgaacgta gccagcactg tttgcagtta accccaattc
     6061 tggaaagcct cgcggcgcag tacaacgggc aatttattct ggcgaagctg gactgcgacg
     6121 cggagcagat gattgccgcg cagtttggtc tgcgtgcgat tccgaccgtg tatctgttcc
     6181 agaacgggca accggtagat ggcttccagg ggccgcaacc ggaagaggcg atccgcgccc
     6241 tgctggataa agtgctgccg cgcgaagaag agctgaaagc gcagcaggcg atgcaactga
     6301 tgcaggaaag caattacacc gatgccctgc cattgctgaa agacgcctgg cagttgtcga
     6361 atcagaacgg ggagatcggc ctgctgctgg cagaaacgct gattgcgctg aaccgttctg
     6421 aagatgcgga agcggtgctg aaaaccattc cgttgcagga tcaggacacc cgctaccagg
     6481 ggctggtggc gcaaatcgaa ctgctgaagc aggcggctga tacgccggaa attcaacagt
     6541 tgcaacagca ggtggcggag aatccagaag atgccgcact ggcgacgcaa ctggcgctgc
     6601 aactgcatca ggttgggcgc aatgaagagg cgctggagtt gctgttcggg catctgcgta
     6661 aagatctcac cgccgcagac ggtcagacgc gtaaaacgtt ccaggagatc ctcgctgcgc
     6721 tgggtacggg tgatgcactg gcgtcgaagt atcgccgcca gctgtatgca ttgttgtatt
     6781 gatattccgt tgatcggcac atgaaccaca acgccggatg cgatgctgcg catccggcat
     6841 cgcatcattt cttcttcaat tgcgtcacca ccaactggtg gcgcgaatta taaaacttac
     6901 gataggttaa atagcaggca ataatggtcg acaagctggc ggttgagagc agcataaagg
     6961 tcaccataat ctgatattta atcgccttca ccggatcaat cccggcaaat atcagcccgg
     7021 acatcattcc tggtaaactc actaagccaa ccgtttttgc tgaatcgacc gtcggaatta
     7081 aagccgcgcg aatactgtcg cgaatcaata tcgctgaagc ctgcttcggc gtcgcaccaa
     7141 gactcagttt ctcctgaatc tgttgctgtt cgctaatgac ccgttgccct aaattgttgt
     7201 aacacaaccc caccgctacc atggcgttac cggcaatcat cccggcgata gggatcacct
     7261 gcatcgggat aaattcaatc gaccctgaga gaatcagcac cgccagggta attcccgccc
     7321 cgaccgtaat ggcaataaac gatgagataa aagctttagc aatatattta ctgcgttttt
     7381 gcgcgttcca cgccgcatta aagcagataa ataacaccat caataatgtc aggctggcat
     7441 catccacgct gaaaatatac ttcagcacat agccgacaat aatcagctga attatcgctc
     7501 gcccgacgct ccagagaata tctttctcca gcgccagttt ttctttatgg ctaattaaga
     7561 ttgccaccac caccagcatt aatgccagtg ctaatgattc gttagtaata ttatgcgagt
     7621 tcatagcgtg cttcctgcat ttctccggca tgcggttgca gtgtaatcac tttatccgca
     7681 tgattaattt cgtctttatc gtgtgtcacc cacagcacgg caatattttg ctcgcgcaca
     7741 taacgatgga tcatctcatt gacgttatgt ttattacttt catccagcgc actggttatt
     7801 tcatccagca ataaaacctt cggcataaat tgcaggttac gaatcaatga gatgcgttgt
     7861 ttttcaccac cagatagctc ggcgatattc ttcgtcaaaa tgctgtccgg caaggcgaag
     7921 cgttcgagaa aatcgagaaa aatggctggg tcaggctgcc ggttacggat ctgccaggga
     7981 aagatcagat tatcgtatac cgtatcgcca aacagcgtcg gtgtctgggc gcagtaagag
     8041 acttgttggc ggtagatttc tggctttagt gtgctgacat cctcaccttc aaacagtaac
     8101 gttccgctgg ttgggctgat caatgaagca actattttta gcagcgtact tttgccacaa
     8161 ccagaaggac cggtaattaa cttaaattcg ccagcacgca gcgaaaaatt gatgttatta
     8221 agaatcttcg catcacccgc cagatatcct acgttttgta gctgaagcaa aggactattt
     8281 tcctgcatcg ctgttccctt tttctgattt ttactaaaaa cagtttatcc ttcgcaggaa
     8341 taagggggaa ctctctttca gtaatcaggt ataatttgtt taatcaagca gcaattttgc
     8401 tgattatgga cggtacaaca ggaggttttt ccgatgctta tctttatccc gattctcatt
     8461 tttgtcgcgc tggtcattgt cggcgcgggt gtcaaaatcg taccgcaggg ctatcagtgg
     8521 acagtagaac gctttggtcg ctataccaaa acgttacagc cggggctcag tctggtggtg
     8581 ccgtttatgg atcgcattgg tcgcaagatc aatatgatgg agcaagtgct cgatatccct
     8641 tcccaggaag ttatctcgaa agataacgcc aacgttacca tcgacgccgt gtgctttatt
     8701 caggtgattg acgcgccgcg cgcggcttat gaagtcagca atctggagct ggcgatcatc
     8761 aacctgacca tgactaacat ccgtaccgtg ctgggttcaa tggaacttga cgaaatgctc
     8821 tctcagcgcg acagcatcaa ctcacgcctg ctgcgtattg tcgatgaagc caccaacccg
     8881 tgggggatta aagtcacccg tattgaaatt cgcgacgtgc gcccaccggc agagcttatc
     8941 tcttcaatga acgcgcagat gaaagcggaa cgtaccaaac gcgcttacat tcttgaagcg
     9001 gaagggatcc gtcaggcgga aatcctcaaa gccgaaggtg aaaaacagtc gcaaatcctg
     9061 aaagcggaag gcgaacgtca gtcggcgttt ttacaggctg aagcgcgtga acgttccgcc
     9121 gaagcagaag cccgcgccac caaaatggtg tctgaagcta tcgcctccgg tgatattcag
     9181 gcggtgaact acttcgtagc gcagaaatac accgaagcgt tacagcagat cggttcctcc
     9241 agtaacagca aagtagtgat gatgccatta gaggccagca gcctgatggg gtcgattgcc
     9301 gggattgccg agctggtgaa agacagcgcc aacaaacgga ctcagccatg atggagttaa
     9361 tggtcgttca tccacatatt ttctggctca gtctcggcgg tttgctgctg gcagccgaga
     9421 tgctgggcgg aaatggttat ttgttgtgga gcggcgtggc agcggtgatt accggcctgg
     9481 tggtctggct ggtgccgctg ggttgggagt ggcaaggggt aatgtttgcc atcctgacgc
     9541 tgctcgccgc ctggctgtgg tggaaatggt tgtcgcggcg ggtgcgcgaa caaaagcaca
     9601 gcgatagtca tttaaaccag cgcgggcagc agctgattgg ccgacgtttt gtgctggaat
     9661 ctccgctggt caacgggcgc ggtcatatgc gcgtcggtga cagttcatgg cctgtcagcg
     9721 ccagcgagga tctcggtgca ggtacgcatg ttgaagtcat tgcgatagaa gggataaccc
     9781 tacatatccg agccgtctcg tcttaatcac cctgcccgat gatgacagca gccggagaga
     9841 ttttcgataa tcgggcagtc ggcgctgtca tcgccagggc aggcattcgc cagtgccagc
     9901 agctggtcgc gcatggattg cagctcctca atgtgtcgtt cgatctccgc caccttctcc
     9961 agcgtgcgcc gtttgacgtc ggcgctgtgc cgctgcgggt cgttaaacag attcaccagc
    10021 tcgccgctct cttccaggtt aaagcccacc tgccgtgcct ggcgcagtaa ggtcagttcg
    10081 ttgagatgct gctgcgtgta ggtgcgataa ccgttttcgc tgcgcatcgg cggcgtcacc
    10141 agccccttct cttcatagaa gcgaatggct ttgctggtca ggccggtaat ttttgctaca
    10201 tcgctgatgt tcatcgttcg cgcaacgcct cctgtgcttt gttaaagggt ttcaacagat
    10261 attcaagcac gtttttttgc cagtgcgaat atcgaccgcc accacaataa tacaggaagt
    10321 actgcgcaca gagagatgtt atacggtttt attgcgcttc atgaccattg ccacaataag
    10381 gctgagtaag agtgagccaa taatccatat caacgcacgg ctaccctggg accacagact
    10441 gtagataaag ccaacgatca ccaacaacat caccaacgta ccaacgataa gaatcggcaa
    10501 cgaggcgtga atatcatgac gcaggcggat cgccaccaca aacaccgcca ggtagcaaat
    10561 caaaaaggtg gcgctggcaa cgctggcgag tgagcctaaa ttcagtgccg ccgtcatcag
    10621 cataatcaac accacgacaa taatgttgcc ccaggtactc tgccgccaca gggatttatt
    10681 cattagcttc ggcagttcgc gttcgctgcc catgttgtcc atgatgttaa acacggcgaa
    10741 caggttcgcg ttaatggctg aagccgtcgc cagtaaagcg ccgatgacga cgatcacata
    10801 acccacatgc ccgagcagcg gagaagcagc ctgcgctacg gcggtatcgg catatttttc
    10861 taactctaat gccgatacat cgctaagcaa aaccagtgcc agcgagatat aaagcaacgt
    10921 ggtaacgcca atcgccacca gaaacgcccg tggcataatg acctgcggat ctttcacttt
    10981 atccgccgcg ttcgccatca tgccaaagcc cgcataggca aggaaagtta tcccaataca
    11041 ggagaagaac gcaccggagc tggggggcgc agagacggaa atatgcgccg gttgcagcga
    11101 ccagacaccg gcaataatca gcaataacag gatcatcatt ttaatgccga cgaggatcac
    11161 ttccagccgc cctaccgcat ggttgcttaa ggagttgaaa agcgtcatca ccgcaatgat
    11221 ccccaacgcg tagagcaaaa taaggtgctc ctcctggctg ccttcatgca aaaactgcac
    11281 ggcataagcg ccaaaagcac gggcgaccat ggcgatgctc accgccagcg tcaacaggta
    11341 cagtaacgag agcgccagcg aaaagacgcc gttgcctaat ccgcgacgaa agaagtcgat
    11401 aatgccgcca ttgctgggat agctcgcccc cagacgcgca taggcataac cggaaaacat
    11461 cgccacaata ccgccaaaag caaaggcgac ccaggtcgag gcttccatta gcaatgcagc
    11521 ctg
//
LOCUS       DAZFZC010000132        11505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFZC010000132 DAZFZC010000000
VERSION     DAZFZC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.133"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            287..1339
                     /gene="yhiM"
                     /locus_tag="ACHKXP_004361"
     CDS             287..1339
                     /gene="yhiM"
                     /locus_tag="ACHKXP_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO7649237.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1998..2432)
                     /gene="pcoE"
                     /locus_tag="ACHKXP_004362"
     CDS             complement(1998..2432)
                     /gene="pcoE"
                     /locus_tag="ACHKXP_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system metallochaperone PcoE"
                     /protein_id="HFO7649238.1"
                     /translation="MKKILVSFVAIMAAASSAMAAETMNMHDQVNNAQAPAHQMQSSA
                     EKSAIQGDSMTMMDMSSHDQAAMSHDMMQNSNSAAHQDMAEMHKKMMKAKPGATNETA
                     KSFSEMSEHEKAAAVHEKANNGQSSVVHQQQADKHRSQITQN"
     gene            complement(2650..4050)
                     /gene="pcoS"
                     /locus_tag="ACHKXP_004363"
     CDS             complement(2650..4050)
                     /gene="pcoS"
                     /locus_tag="ACHKXP_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance membrane spanning protein
                     PcoS"
                     /protein_id="HFO7649239.1"
                     /translation="MRFKISLTTRLSLIFSAVMLTVWWLSSFILISTLNGYFDNQDRD
                     FLTGKLQLTEEFLKTETFRNKTDIKSLSEKINDAMVGHNGLFISIKNMENEKIVELYA
                     KNSVVPAVLLNKSGDILDYMIQTEENNTVYRSISRRVAVTPEQGKSKHVIITVATDTG
                     YHTLFMDKLSTWLFWFNIGLVFISVFLGWLTTRIGLKPLREMTSLASSMTVHSLDQRL
                     NPDLAPPEISETMQEFNNMFDRLEGAFRKLSDFSSDIAHELRTPVSNLMMQTQFALAK
                     ERDVSHYREILFANLEELKRLSRMTSDMLFLARSEHGLLRLDKHDVDLAAELNELREL
                     FEPLADETGKTITVEGEGVVAGDSDMLRRAFSNLLSNAIKYSPDNTCTAIHLERDSDC
                     VNVMITNTMSGQVPANLERLFDRFYRADSSRFYNTEGAGLGLSITRSIIHAHGGELSA
                     EQQGREIVFKVRLLMD"
     gene            complement(4047..4727)
                     /gene="pcoR"
                     /locus_tag="ACHKXP_004364"
     CDS             complement(4047..4727)
                     /gene="pcoR"
                     /locus_tag="ACHKXP_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     PcoR"
                     /protein_id="HFO7649240.1"
                     /translation="MQRILIVEDEQKTGRYLQQGLVEEGYQADLFNNGRDGLGAASKG
                     QYDLIILDVMLPFLDGWQIISALRESGHEEPVLFLTAKDNVRDKVKGLELGADDYLIK
                     PFDFTELVARVRTLLRRARSQAATVCTIADMTVDMVRRTVIRSGKKIHLTGKEYVLLE
                     LLLQRTGEVLPRSLISSLVWNMNFDSDTNVIDVAVRRLRSKIDDDFEPKLIHTVRGAG
                     YVLEIREE"
     gene            complement(4782..5660)
                     /gene="pcoD"
                     /locus_tag="ACHKXP_004365"
     CDS             complement(4782..5660)
                     /gene="pcoD"
                     /locus_tag="ACHKXP_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance inner membrane protein PcoD"
                     /protein_id="HFO7649241.1"
                     /translation="MVIFGLPFFQIYGISGVRHETYNLTNFRSFITFAVVTGIILTGI
                     NMLLVSNAMSGVTDLRELSIHVIEMVIEETDVGISWIVRLCALFTTLGALFLYTNKRV
                     LSCLLMTMSGGVALATLAWGGHAVMHDGLHYYLHLLSDLTHLGAAGAWTGALVAFAIL
                     LMRRNEHNAQSVIVISDSLAKFATAGTVIVVALILSALVNYLYIAEGNLTPLFNSSWG
                     RILLAKTALFVLMLLLAAANRFHLGPRLEVMVREGNYDRSVALMRNSILTEFVVAIII
                     LGAVAWLGMLAPSQVS"
     gene            complement(5716..6096)
                     /gene="pcoC"
                     /locus_tag="ACHKXP_004366"
     CDS             complement(5716..6096)
                     /gene="pcoC"
                     /locus_tag="ACHKXP_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system metallochaperone PcoC"
                     /protein_id="HFO7649242.1"
                     /translation="MSILNKAILTGGLVMGVAFSAMAHPELKSSVPQADSAVAAPEKI
                     QLNFSENLTVKFSGAKLTMTGMKGMSSHSPMPVAAKVAPGADPKSMVIIPREPLPAGT
                     YRVDWRAVSSDTHPITGNYTFTVK"
     gene            complement(6136..7026)
                     /gene="pcoB"
                     /locus_tag="ACHKXP_004367"
     CDS             complement(6136..7026)
                     /gene="pcoB"
                     /locus_tag="ACHKXP_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance outer membrane transporter
                     PcoB"
                     /protein_id="HFO7649243.1"
                     /translation="MKRNLKAIPVLVAGLFTSQLSIAAGSVSADPHAGHDMSAMQMPA
                     DENFTEMTSMEPIVTESRTPIPPVTDADRKAAFGNLQGHAIHDSAINYLVLLDQLEWQ
                     RSDNTNNFSWSVNSWIGGDTDRIWLKSEGERSNGETEAAEAQLLWGHAVGPWWDLVAG
                     VRQDFRPASARTWAAVGFQGLALYNFESEITGFVSNGGKAALRLGGEYDVLLTNRLIL
                     QPSYEVNFYSQDDESRGRGRGLTDTELGLRLRYEIRREFAPYIGVSWNQLYGKTSDMA
                     KREGEKDHQVVFLAGARIWF"
     gene            complement(7032..8849)
                     /gene="pcoA"
                     /locus_tag="ACHKXP_004368"
     CDS             complement(7032..8849)
                     /gene="pcoA"
                     /locus_tag="ACHKXP_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase PcoA"
                     /protein_id="HFO7649244.1"
                     /translation="MLLKTSRRTFLKGLTLSGVAGSLGVWSFNARSSLSLPVAASLQG
                     TQFDLTIGETAVNITGSERQAKTINGGLPGPVLRWKEGDTITLKVKNRLNEQTSIHWH
                     GIILPANMDGVPGLSFMGIEPDDTYVYTFKVKQNGTYWYHSHSGLQEQEGVYGAIIID
                     AGEPEPFTYDREHVVMLSDWTDENPHSLLKKLKKQSDYYNFNKPTVGSFFRDVNTRGL
                     SATIADRKMWAEMKMNPTDLADVSGYTYTYLMNGQAPLKNWTGLFRPGEKIRLRFING
                     SAMTYFDIRIPGLKMTVVAADGQYVNPVTVDEFRIAVAETYDVIVEPQGEAYTIFAQS
                     MDRTGYARGTLATREGLSAAVPPLDPRPLLTMEDMGMGGMGHDMAGMDHSQMGGMDNS
                     GEMMSMDGADLPDSGTSSAPMDHSSMAGMDHSRMAGMPGMQSHPASETDNPLVDMQAM
                     SVSPKLNDPGIGLRNNGRKVLTYADLKSRFEDPDGREPGRTIELHLTGHMEKFAWSFN
                     GIKFSDAAPVLLKYGERLRITLINDTMMTHPIHLHGMWSDLEDENGNFMVRKHTIDVP
                     PGTKRSYRVTADALGRWAYHCHLLYHMEMGMFREVRVEE"
     gene            9084..9533
                     /locus_tag="ACHKXP_004369"
     CDS             9084..9533
                     /locus_tag="ACHKXP_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004388333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein"
                     /protein_id="HFO7649245.1"
                     /translation="MNILITTTAFTALFCGAAFAQSSDIAHEAHRFVNNASAVSHVNS
                     STHENLPDRVNKNNTPSFSEMNEHERAIVAHSFMNNSASYAHQKMIEEHKKMLSGSDA
                     NSKTSSSSFNELNAGEKAALVHEQVNNAGAEAHQTQARKLRGLYSTR"
     gene            9822..10559
                     /locus_tag="ACHKXP_004370"
     CDS             9822..10559
                     /locus_tag="ACHKXP_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFO7649246.1"
                     /translation="MYSTDVVKENAYLSATRSGLESNEIATLQRSLPSRFNLRHLKKN
                     ESLKLVLQKKAGKSRVVAYKFTSGSFNYTAYRISDKKFYNLSDTSGKGSLDYPLPATA
                     RLSSPFNPARLNPVSGKVSPHNGIDYSMPMNTKIVSVIDGKITRAEYNSTMGYFVEVT
                     GKAGVKTRYLHLNKILVTKGARVTRGGAIALSGNSGRSSGPHLHYELVINNNPVNSLA
                     FRAAAPADNKLEQHAFAHARDYERYLD"
     gene            complement(10593..10790)
                     /locus_tag="ACHKXP_004371"
     CDS             complement(10593..10790)
                     /locus_tag="ACHKXP_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HFO7649247.1"
                     /translation="MKSTTYALIAVAAIAAFALLREHWSHVAGYWPYLLLLVCPLMHL
                     FHGHGGHGDHQHHGSENDKKN"
     gene            complement(10831..>11505)
                     /gene="silP"
                     /locus_tag="ACHKXP_004372"
     CDS             complement(10831..>11505)
                     /gene="silP"
                     /locus_tag="ACHKXP_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag(+)-translocating P-type ATPase SilP"
                     /protein_id="HFO7649248.1"
                     /translation="NSIVIDNQKAVADTLRMEGATVIYVATDGDLAGLIAISDPVKTT
                     TPDALKALRQAGIRIVMLTGDNQLTAEAVARKLGIDEVEAGILPDGKKAVITRLKESG
                     HVVAMAGDGVNDAPALAAADVGIAMGTGTDVAIESAGVTLLKGDLMILNRARHLSEIT
                     MKNIRQNLFFAFIYNALGVPVAAGLLYPVYGILLSPVIAAAAMALSSVSVIVNALRLK
                     SVRLGK"
BASE COUNT         2956 a         2900 c         2596 g         3053 t
ORIGIN      
        1 gttcgctgat tttaagcacc gagactatcc ggtcttttac caaataatga agaataatcc
       61 ctccctggaa gataattgta aaaaacaaca gcataagaat taatcttagg ataaattttt
      121 atttatcatg gccttttgaa cgctgaaaag tagcgctctg caaagtaaac cccgctatag
      181 ttatgctatg catttgtctt taatgatatc tcgtcattat attaacagga tgaaattatc
      241 attaatgcat atttcaatat tagcagggat acctgaggag tttaaagtga acatatatat
      301 cgggtggctt ttcaaattaa tccctttgat tatgggctta atttgtatcg cgttaggtgg
      361 ctttgtgctg gaaagttcag ggcaaagcga gtatttcgtc gcgggtcatg tgctgatttc
      421 tctggcggcc atatgcctgg cattattcac taccgcattt attatcattt cgcagctcac
      481 gcgcggcgtt aatacgtttt acaatacatt gttccccatt attggctatg cggggtcaat
      541 tatcaccatg atatggggtt gggcactgtt agcaggcaat gatgtgatgg cagacgagtt
      601 tgtcgccggc catgttattt tcggcgttgg tatgattgcc gcctgtgtat cgacggtggc
      661 agcgtcatcc ggtcactttc tgctcattcc caaaaatgca gcggggagca agagcgacgg
      721 aacaccggta caggcttatt cttcattaat cggtaactgc ctcattgccg ttcccgtttt
      781 actcaccctg ctcggtttca tttggtctat tacgctgtta cgtagtgctg acataactcc
      841 gcattatgtc gcgggtcacg tattgcttgg gttaaccgca atctgtgcct gtctaattgg
      901 ccttgttgcc acaattgtcc atcaaacacg taatacgttt tcaactaaag aacactggct
      961 gtggtgttat tgggttattt ttctcggctc aatcacggta ctgcagggga tatacgtctt
     1021 agtcagttcc gatgcaagcg cccgactggc tcccggcatt attcttattt gcctcggaat
     1081 gatctgttac agcatattct caaaagtctg gctactggca ctggtatgga gacgtacctg
     1141 ttcgttagcc aacagaatac cgatgattcc cgtcttcacc tgcctgtttt gccttttcct
     1201 ggcatcgttt cttgcggaaa tggcgcagac cgacatggga tattttattc cttcgcgagt
     1261 tctggtcggt ttgggagcgg tatgctttac gttgttctca atcgtttcaa tattagaagc
     1321 gggttctgct aaaaaataat tgcaacgtac cggataaaac cagcgttgac catttgcgta
     1381 acgctggttt ttcttaggca tcatgaaata acgcagcatt aatgcatagt ggttaagtat
     1441 aaaaaacagc aaagttactg tttttttcaa cctgttcata tttcataaag atctggccac
     1501 tgggtgagtt ttcaatcacc tttccatcca ccttatatta agcatggagg gtttcagttc
     1561 acgggctcat tagaaaataa tcacaagata accactatca aaattagctc atttttaatg
     1621 cgactctaat aattttcatc tttaggaaat aggtcaggac gactttgctg caattgtgta
     1681 gagacattaa agttgtatcc tttaacctga aaaagacttt aaagttgtaa agttttaaaa
     1741 aaacacatac aaaacatgat gctaagtgtg tatcacacgc tgttccaaca ttatgccttg
     1801 atgttcttgc aggaactgca atttacagga ggaaaaaggt gtttttgtag ttatctggcg
     1861 ttacgtgaca gtgttggtca gatacttcca cctcttcaga gttaaagttt gatatatggg
     1921 aaaaaaaacg ctcccggacg ggagcgtaag ccagtgactt cggcgtcaaa tgagggtcta
     1981 acaagtggag ctgcgggtta attctgggtg atctgactgc gatgcttatc agcctgctgc
     2041 tggtgaacaa cggaagactg accattattc gccttctcat gtacagctgc ggccttctca
     2101 tgctcgctca tttcagaaaa tgactttgct gtttcgttgg tagctccggg tttagctttc
     2161 atcatttttt tatgcatttc agccatgtcc tggtgggcag cagaattgct gttttgcatc
     2221 atgtcatggg acattgcggc ctgatcgtga ctgctcatat ccatcattgt catgctgtct
     2281 ccctgaattg cacttttttc agcagatgac tgcatctggt gggcaggtgc ctgtgcatta
     2341 tttacctggt catgcatgtt cattgtctct gcagccatgg cagatgaagc ggcagccata
     2401 atggcaacaa atgatacgag aatctttttc atggttgggt ctccggtgtt tacataccca
     2461 aacaatcaat ggcttataac agagaaagca gttgatctca gggcgggtta atcatcactc
     2521 caggaaccag gtgattgtgt tatcacgctg ctcctgattt atgaataatt ataaaaaacc
     2581 gcctctgttt atcgcgtgac tgaatgatga caattttgtc accttccagg tttctcctga
     2641 acaacgggat taatccatta acaggcgcac tttgaacaca atttcacgcc cctgctgttc
     2701 tgctgacagc tcgccgccgt gagcatgaat gatcgacctt gtaattgata atcccagccc
     2761 cgcgccttcc gtgttgtaga accttgatga gtctgcgcga tagaaccggt caaacaaacg
     2821 ttccagatta gcgggaacct ggccggacat cgtattcgta atcatcacgt tcacacagtc
     2881 actgtcacgc tcaaggtgta tcgctgtaca ggtgttatcg ggagaatact tgattgcatt
     2941 ggaaagcagg ttactgaaag cacgtcggag catatcgctg tctccggcaa caacgccctc
     3001 tccttcaacc gtgattgtct ttcctgtttc gtctgccagg ggctcgaaca actcacgtaa
     3061 ttcattcagt tcggctgcca gatccacatc atgtttatcc agccgcagca gaccatgctc
     3121 tgaacgtgcc agaaaaagca tgtcactggt cattcgtgac aaccttttca gttcttccag
     3181 gttagcgaat aaaatttcgc ggtaatgcga aacatccctt tccttagcca gtgcaaactg
     3241 cgtctgcatc atcagattac tgactggtgt gcgcagctca tgcgcgatgt cagacgagaa
     3301 atctgacagt ttccggaatg ccccctccag gcgatcaaac atattattga actcctgcat
     3361 ggtctcagag atttccggcg gagccagatc gggatttaga cgctgatcca ggctgtgtac
     3421 ggtcatggag gaagccagac tggtcatttc ccgtagcggt ttcagaccaa tacgtgtggt
     3481 cagccagccc agaaaaacag aaataaagac cagaccgata ttgaaccaga acagccaggt
     3541 actgagtttg tccataaaca gggtgtgata cccagtatcc gtggcaaccg taatgatgac
     3601 atgtttgctt ttaccctgtt ccggcgtcac ggcaacccgc cgcgagatac tgcggtacac
     3661 ggtgttattt tcttccgtct ggatcatata gtcgagaata tcacccgact tattaagcag
     3721 gaccgctgga acaacagaat ttttggcata gagttcaaca attttttcat tttccatgtt
     3781 ttttatagaa atgaataagc cattgtgccc caccatcgca tcgtttattt tttctgataa
     3841 tgacttaata tccgttttgt tcctgaacgt ctctgtttta agaaactctt cggtgagctg
     3901 aagtttacct gtcagaaaat cgcggtcctg attatcgaaa tagccattaa gggtgctaat
     3961 caggataaaa cttgataacc accataccgt aagcatcacc gcagaaaaaa tcaggctcag
     4021 gcgtgtggtc agggaaattt tgaacctcac tcttctctga tctccaggac atatccggca
     4081 ccgcgaacgg tatggatcag ttttggctca aagtcatcat caattttact tctcagacgt
     4141 ctcacggcga catcaatcac attcgtatca ctgtcaaaat tcatgttcca gaccagggac
     4201 gagataagac tcctgggtaa cacttctccg gtgcgttgca gcagcaactc aagcagaacg
     4261 tattctttac cggtgagatg gatcttcttc cccgaacgga tcacggtccg gcgcaccata
     4321 tcaacggtca tatcggcgat ggtgcagact gttgcggcct gcgagcgtgc ccggcgcagt
     4381 agggttctta cacgtgcaac cagctccgta aaatcaaagg gcttaatcag gtagtcatct
     4441 gcgccaagct ccagtccttt cactttgtcc cgcacgttgt cctttgcggt taaaaacagg
     4501 accggttctt cgtgcccgga ctccctcagt gcgctgatga tttgccaccc gtcgaggaaa
     4561 ggcagcatca cgtccagtat tatcaaatca tactgtccct tcgacgcggc cccgagacca
     4621 tcgcggccat tattaaagag atcggcctga tagccttcct caaccagtcc ctgctgcagg
     4681 taacgacctg ttttttgttc gtcttcaacg attaaaatac gctgcatggt caactcgctg
     4741 atatgaaagt aaaaatctca cgcatgagct ttggctgtcc cctagctgac ctgagacgga
     4801 gcaagcattc cgagccacgc tacggcgccc agaatgataa tcgcaacaac gaattctgtc
     4861 aggatgctgt ttcgcatcag ggcaacgctg cgatcataat tcccttccct gaccataact
     4921 tcaagccggg gacccaggtg aaaccggttt gctgcagcca gaagaagcat cagaacaaac
     4981 agagccgtct tggcaagcaa tatcctcccc caggaactgt tgaataaggg agttaagtta
     5041 ccctcagcaa tatacagata gttgaccagc gcactcagga tcagggctac aacaatcacc
     5101 gttcctgccg tggcaaattt tgccagggag tcagatatca caatgacgct ctgtgcatta
     5161 tgctcgtttc tgcgcatcag caggatagca aatgcaacca gagcacctgt ccaggcacct
     5221 gcagcgccga gatgggtcag atcgctcagt aaatggagat agtaatgcag accgtcatgc
     5281 ataacggcgt gtcctcccca ggcaagtgta gccagcgcca cgcccccact catcgtcatc
     5341 agcaggcagg acaatactct cttattagtg taaaggaaca aagcaccgag tgtggtaaac
     5401 agggcacaga gcctgacaat ccagctaata cccacatcag tttcttctat caccatctcg
     5461 ataacatgga tggataattc tctgaggtca gttactccac tcatggcatt agataccagg
     5521 agcatattaa tgccagtaag aatgatgcct gtaacaacag caaaagttat aaacgacctg
     5581 aaattagtca ggttataggt ttcatgtctg acaccgctta ttccatatat ctgaaaaaat
     5641 ggcaatccaa atattaccat caaatccaga taaagaagaa aacgaataac aatcataatc
     5701 aggtcgttca taatattact tcactgtaaa ggtgtaatta ccggtaatag ggtgcgtatc
     5761 tgaagaaacc gcgcgccagt caacacgata agtgccagcg ggtaaaggct ctcgcggaat
     5821 aatgaccatc gatttagggt cagcgcctgg cgccactttt gccgcgaccg gcatcggaga
     5881 atgtgatgac atgcctttca tacccgtcat cgttaatttt gcacctgaga atttcacggt
     5941 cagattttcc gagaaattaa gctgaatctt ttccggggcc gctacggctg aatcagcctg
     6001 tggcacagag ctttttaatt ccggatgggc catagcagag aaagcaacgc ccataacgag
     6061 gccacctgta agaatggctt tatttaaaat cgacatttta tttacctgtt tagttgagtg
     6121 ttttatatca gtgcgttaaa accagattct ggctcccgcc aggaatacta cctgatggtc
     6181 tttctcacct tctcttttcg ccatatcgga tgttttcccg taaagttgat tccaggaaac
     6241 gcctatatag ggtgcaaact cacggcgtat ttcatagcgc agccggagcc ccagctctgt
     6301 gtcagtcagt cccctgccgc gaccccgcga ttcatcatcc tgactgtaga aattcacctc
     6361 ataggatggc tggagtatga gccggttagt cagtaaaacg tcgtattctc ctcccagacg
     6421 aagggctgct tttccgccat tactgacaaa acccgtaatt tcagactcaa aattatagag
     6481 tgccagcccc tgaaaaccga cagcagccca ggtccgggca gaagcaggtc tgaaatcctg
     6541 cctgacaccc gcaaccaaat cccaccatgg gccaaccgca tgtccccaga gtaactgcgc
     6601 ttcagccgcc tccgtttccc cattgcttcg ttcaccttca ctctttagcc aaatccgatc
     6661 tgtgtcgcct ccaatccagc tgttaacact ccagctgaaa ttgttggtgt tatccgaccg
     6721 ttgccattcc agttgatcca gcagaaccag ataattaatc gcactgtcgt gaatcgcatg
     6781 cccctgtaaa ttgccgaatg cagccttccg gtcggcatcg gtaacaggcg gaattggcgt
     6841 tctgctctca gttacaatgg gctccattga cgtcatctca gtgaaattct catctgctgg
     6901 catctgcatg gcagacatgt cgtgcccggc gtggggatct gcagagacgg agcccgccgc
     6961 aatagaaagc tgtgaggtaa acaaaccggc gaccagaaca ggtatggcct tcaaatttct
     7021 cttcattcgc atcattcctc cacccggact tcacgaaaca ttcccatttc catgtgatag
     7081 agcaaatggc agtgatacgc ccagcggcca agcgcatctg ctgtcactct gtaactgcgt
     7141 tttgtaccag ggggaacatc tattgtgtgt ttacgaacca tgaaattacc gttttcatct
     7201 tccagatcgc tccacatacc atgcaggtga atggggtgag tcatcatggt atcgttgatc
     7261 agcgtgatcc tgagccgctc accgtatttc agcagcaccg gtgcggcatc tgaaaacttg
     7321 attccgttaa atgaccaggc aaacttttcc atgtggccgg ttaaatgcag ttctatggta
     7381 cggccaggtt cacgtccgtc aggatcctca aagcggcttt tcaaatccgc gtacgtgaga
     7441 acctttcttc cgttatttcg aagaccaata cccggatcat ttaatttcgg agagacgctc
     7501 atcgcctgca tatcaaccag tgggttatcc gtttctgacg caggatgact ttgcataccc
     7561 ggcattccgg ccatccggga atgatccata ccggccatgc tgctgtgatc catgggcgcg
     7621 gaggatgtcc cgctatccgg aaggtcagca ccgtccatag acatcatctc tccgctgtta
     7681 tccatgcctc ccatctggct gtggtccatt cctgccatat catgtcccat tccccccata
     7741 cccatatctt ccatggtcaa cagaggacgg ggatcgaggg ggggaacggc agcacttaac
     7801 ccctctctcg tggccagtgt ccctcgagcg taaccggtcc tgtccatgga ttgtgcgaag
     7861 atggtatagg cctcaccctg aggctccaca atgacatcat aggtttcggc aacggcaatc
     7921 ctgaattcgt caacggtaac cgggtttaca tactggccat ctgcagccac gaccgtcatt
     7981 ttcagcccgg ggatacggat atcgaaatag gtcattgccg agccgttgat aaaccgtaag
     8041 cgtatctttt caccgggacg gaacagtccg gtccagtttt tcagcggggc ctgcccgttc
     8101 atgagatagg tgtaggtgta gccactgaca tccgcgaggt cagtcggatt cattttcatt
     8161 tcagcccaca ttttccgatc ggcaatggtg gctgacagcc ccctggtatt cacgtcgcgg
     8221 aaaaaagagc caacggttgg tttattgaaa ttgtagtaat ccgactgttt ttttaatttt
     8281 ttcagcaggc tgtgaggatt ttcatcggtc cagtcagaca acatgaccac atgctcacga
     8341 tcgtaagtaa acggttctgg ctccccggca tcgatgataa tggcaccgta taccccctcc
     8401 tgttcctgca gaccggaatg gctgtggtac cagtaagtcc cgttctgctt aaccttaaag
     8461 gtgtaaacgt aggtatcatc aggctctatg cccataaaac tcagccccgg aacaccatcc
     8521 atattggccg gaagaataat gccgtgccag tgaatggacg tctgttcatt aagacggttt
     8581 ttgaccttca gggtaatggt gtcaccttct ttccagcgaa gaacgggccc cggcaggcct
     8641 ccattgattg ttttggcctg acgctcactg cccgtgatat tgacggccgt ttcaccaatg
     8701 gtcaggtcaa actgagtacc ctgcagggat gcggcaactg gcaggctcag actggaacgc
     8761 gcattgaaac tccatacgcc aagacttccg gctacgccag agagggttaa ccccttcagg
     8821 aaagttcgtc gagacgtttt caacagcatg cgcattccct tatttaaagt atggttactg
     8881 acagaattcg agaaccgatt taaattaaat tactggttca tccacttaca atgaaatgaa
     8941 tctagcacac cttaagaaac aaattcatta caatcgtgta atgtacatag ctgtattaca
     9001 tttacgtcat cttccccaca ggttgattat ttttatatat gatcataagg acatctttta
     9061 tgtacctcag aaggtaatta cacatgaata tattaatcac gaccactgcg tttacagctt
     9121 tattttgtgg ggcagctttt gctcagtcca gtgatattgc ccatgaagca catcgatttg
     9181 ttaataatgc ctcagccgtc agtcatgtga attcctcgac gcatgaaaac ttaccggaca
     9241 gggttaataa aaacaacacg ccctcattct ctgaaatgaa tgaacatgaa agggccattg
     9301 ttgctcattc atttatgaac aacagcgcgt cctatgcgca tcagaaaatg attgaggaac
     9361 ataaaaaaat gctgtccggc agtgatgcaa attcaaagac ctcgtcttct tcttttaacg
     9421 aactgaatgc cggagaaaaa gccgctctcg tgcatgagca ggtcaataat gccggtgcgg
     9481 aagcacatca gacgcaggca agaaagcttc gcgggctgta ttcgaccagg taactgcagg
     9541 cgggttagtg cttagcgtca ggtgcgcagc agggctttac tgacgggtta cgtagcagga
     9601 catgagcccc atattgctct gccgtaacct gtttcagtcc cgttaatcat caccgtcggg
     9661 ctggtcatac agttcccaga gcttcaggag caaacgggaa acaagatatg ttacaaaaat
     9721 gacgaccatc aacggtgctc ccgattaact gacagatgac acgcctcctg aaaaccccta
     9781 gcatacgccg cagtgttcat gctaacggcg aattccagta aatgtattct accgatgtcg
     9841 taaaagaaaa tgcctacctt tcagccaccc gctcggggct ggaatcgaac gagatcgcta
     9901 ctcttcagcg ctccttgcct tcccggttta atctgcggca tttgaaaaaa aatgaatcat
     9961 taaaactcgt actgcaaaag aaagcgggaa aatcacgtgt cgtggcctat aaatttacgt
    10021 ccggttcatt taattacacg gcgtatcgta tatcagataa aaagttctat aacctttccg
    10081 atacttccgg gaaaggcagt ctcgattatc cgttaccggc cacagcaaga ctcagttcgc
    10141 ctttcaatcc tgcaagactt aacccggtat cgggaaaagt gagtccccat aatggcattg
    10201 attattccat gcccatgaac acgaaaatag tcagcgtcat cgacggaaaa atcacccggg
    10261 ccgaatacaa cagtaccatg ggatattttg ttgaagtaac gggaaaagcc ggtgttaaaa
    10321 ctcgctatct ccacctcaat aaaatactcg ttactaaagg ggccagggtt acacggggag
    10381 gtgctattgc gttatccggt aacagcggac gttcatccgg tcctcatctg cattacgagc
    10441 tggtcatcaa taacaaccct gttaactctc tggcgttccg ggcagcggca cccgctgata
    10501 acaaactcga acagcatgcc tttgcgcatg ccagagacta cgaacgatac ctggactgat
    10561 aacgggaccg cgacgcggcc ccgtctgccg gattaatttt ttttatcgtt ttcacttccg
    10621 tgatgttgat gatctccatg ccctccgtgg ccgtggaaaa gatgcattag cgggcagacc
    10681 agcaataaca gatatggcca gtaacctgcc acatgtgacc agtgttcgcg caggagggca
    10741 aatgccgcga tcgcggcgac agcaataagc gcataggtgg tacttttcat actggactcc
    10801 ttctgttcgt aactgaccct tcactcagtg ttatttcccg agcctgacac ttttcagacg
    10861 caacgcattc acaatgacgc tgacggagga aagagccatg gccgccgccg caataactgg
    10921 cgacagcagt attccataca caggataaag cagacctgca gccacaggca cgccaagtgc
    10981 gttgtagata aatgcaaaaa acagattctg acggatattt ttcatggtga tctctgacag
    11041 atgacgggcc ctgttcagta tcatcaagtc gcctttgaga agggtgactc cggcactttc
    11101 aattgccaca tctgtacccg ttcccatggc tatacccacg tcagccgctg ccagcgccgg
    11161 ggcatcattc acaccgtctc cggccatcgc aaccacatgg ccagactctt tcagtcgggt
    11221 tatcactgct tttttgccat ccggcagaat tccggcttca acctcatcta ttcccagttt
    11281 ccgtgcgact gcttcagcag taagctggtt atccccggtg agcataacga tgcggatccc
    11341 cgcctgacgc aaagctttaa gcgcatccgg cgtggttgtt ttcacgggat ccgagatagc
    11401 tatcaggcct gcaaggtccc cgtctgtggc cacatagata acggtagcgc cttccatccg
    11461 caacgtatcc gcaacggcct tttgattatc aataactata ctgtt
//
LOCUS       DAZFZC010000133        11444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFZC010000133 DAZFZC010000000
VERSION     DAZFZC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.134"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(246..1433)
                     /locus_tag="ACHKXP_004373"
     CDS             complement(246..1433)
                     /locus_tag="ACHKXP_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO7649249.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1433..1798)
                     /locus_tag="ACHKXP_004374"
     CDS             complement(1433..1798)
                     /locus_tag="ACHKXP_004374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649250.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            2035..2382
                     /gene="tnpB"
                     /locus_tag="ACHKXP_004375"
     CDS             2035..2382
                     /gene="tnpB"
                     /locus_tag="ACHKXP_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7649251.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            2432..3970
                     /locus_tag="ACHKXP_004376"
     CDS             2432..3970
                     /locus_tag="ACHKXP_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO7649252.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRPRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTRRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGRITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIA
                     LPAE"
     gene            complement(4033..4221)
                     /locus_tag="ACHKXP_004377"
                     /pseudo
     CDS             complement(4033..4221)
                     /locus_tag="ACHKXP_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4274..5272)
                     /gene="lpxM"
                     /locus_tag="ACHKXP_004378"
                     /gene_synonym="msbB"
     CDS             complement(4274..5272)
                     /gene="lpxM"
                     /locus_tag="ACHKXP_004378"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7649253.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            complement(5346..7067)
                     /gene="cptA"
                     /locus_tag="ACHKXP_004379"
     CDS             complement(5346..7067)
                     /gene="cptA"
                     /locus_tag="ACHKXP_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7649254.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            complement(7161..8267)
                     /locus_tag="ACHKXP_004380"
     CDS             complement(7161..8267)
                     /locus_tag="ACHKXP_004380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7649255.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            complement(8267..9088)
                     /locus_tag="ACHKXP_004381"
     CDS             complement(8267..9088)
                     /locus_tag="ACHKXP_004381"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7649256.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            complement(9689..9823)
                     /locus_tag="ACHKXP_004382"
     CDS             complement(9689..9823)
                     /locus_tag="ACHKXP_004382"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649257.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            10561..11213
                     /locus_tag="ACHKXP_004383"
                     /pseudo
     CDS             10561..11213
                     /locus_tag="ACHKXP_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    10842..10958
                     /locus_tag="ACHKXP_004383"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         2967 a         2957 c         2740 g         2780 t
ORIGIN      
        1 gagggaaagc tgctattacc ttcttcagag agcccccagc tacttcctgt gactctcgat
       61 cgataggaat ttaaatccca aaaagattaa aaaaacaccc aaaaacggat gtttattcaa
      121 cactgcctgc gtgccatgag tgcaccgccg tcaattaaat ggatcttatc gattgatact
      181 gcatatcact gatggttttt taccccattc ccctgaaaat ggacgcaact tctccgatgc
      241 ggctctcagc cgcacacccg ctgttgcgcc agtggctcat gcatcaggac cagctctgtc
      301 agacggtagc cccgcttcag ccccgtaaaa cgcatctgac ctccgcacag gatgcacttc
      361 agcgggtcaa ccttcagtaa ccgctgatac atccctctcc acctgatttg catcgccgtc
      421 tttctcaccg tctccgttat gacgtacaca acatcttcca gtaaccggcg cttcaccgga
      481 ctcaggaaac cgtagtacct caccatacgg aaccccttat ccgccacatg ccaggagaac
      541 ctttccataa actcatcacc actcatcacc aggtattctt cccgttttgt ccggtgactg
      601 ttgtaacgca gaccaatttc atcctgacca gcatagtgct ccagacggct catcggcacc
      661 ggcggttttt tcaggtaaga gccgaagtac accgccacat gtgtggcgtt atccatcacc
      721 cgggatacgt tgacattcca gccccgccgg taatgactgt ccaggaagcg attccagtcc
      781 cgtttactgc ttccttctgc tgccagcgca tccggtatca ccaggtccgg atatttccgt
      841 gacagtaacc gcgttatccg gtaacgccac atactcatca ccttacgggc gtaaaaatga
      901 aggtttttcc aggtgtggtc tgatgtcacg ccgccggtcg ttgtcgacag gtgaatgtgc
      961 ggatgccact cctggtcacg tccccatgtg tggatcaccg tgaatatccc cggcaccaca
     1021 tctgcctggc ggcagatttc ctgtatcaca tccgcagcaa tgcggctcat ctctgccagt
     1081 aacctccggt tgtggaacac tagggaccag tactggcagg gaagtgtgaa cacaatatgc
     1141 tgccacggac agtcgggaac cagactcagc agatactgta tccactgtgc gccagccttc
     1201 actccgcagt gcgggcagga gcgactttta caccggaagc agaccttttt tatgtggctg
     1261 cagtccggag atgaacagca ccactgtgtg taccccatca gcgtggtccc gcaggccatg
     1321 atttttgtca cagactcaat gactaccgga cgcactgaac cttccggttg tttctccagc
     1381 cagttaagcc agcggtttcc ctgctgaaaa atgtcggcaa aacggggaag catcagaagg
     1441 gcggggggac tccgtccggc cagtgaaccg tgccacactc cgggcagtac atgccgccgg
     1501 cgctgatacc ggcaagaatg gtcgcaaact cccgctccgt gcagcgggct atttcaggat
     1561 acccttcgtc atcaacacgt acaaaccaga agaccagctt tttgtttccg gcatccacaa
     1621 agaagggaat attcaggtct gcgcagcact caacggcatc gtcaaaatta tcaaagcgca
     1681 gagcttctgc gtcttcttcg tcaaagaagt catcgtcgtg aagcttcacg acatagcggg
     1741 gaagtttgct tctttgaggg gcaggttttc gtttacgggg tttagctgaa cgggacatat
     1801 aactacctga aagacaatga tattgtctgt ttttataacg gtaatttcag gccatgacaa
     1861 gccgcagccg ccaggctgcc tactcgatgc agctgccgaa cagccagaat cgctcgcaga
     1921 ggacccggaa accctcagta tcagctgtga ggtaacgttc cggcacggga cgctccgctt
     1981 caatggcaat gtcagcgaaa agctcctgac tctgctgata caggaactga agcgatgatc
     2041 ccgttacctt ccgggaccaa aatttggctg gttgccggta tcaccgatat gagaaatggc
     2101 ttcaacggcc tggctgcgaa agtacagacg gcgctgaaag acgatcccat gtccggccat
     2161 gttttcattt tccggggccg cagcggcagt caggttaaac tgctgtggtc caccggtgac
     2221 gggctgtgcc tcctgaccaa acggctggag cgtgggcgct tcgcctggcc gtcagcccgt
     2281 gatggcaaag tgttccttac gcaggcgcag ctggcgatgc tgctggaagg tatcgactgg
     2341 cgacagccta agcggctgct gacctccctg accatgctgt aaatctcttt atcctggttg
     2401 tcacagaata agcccggtaa aatacgggct tatgaacgac atctcttctg acgacatctt
     2461 cctgctgaaa cagcgcctgg ccgaacagga agcgctgatc cacgccctgc aggaaaagct
     2521 gagcaaccgg gagcgcgaaa tagaccatct gcaggcgcag ctggataaac tccgccggat
     2581 gaacttcggc agtcgttccg aaaaagtctc ccgccgtatc gcacaaatgg aagccgatct
     2641 gaaccggctt cagaaagaga gcgatacgct gactggtagg gtgtatgacc cggcagtaca
     2701 gcgtccgttg cgtcagaccc gcccccgtaa gccgttccct gaatcactac cccgtgacga
     2761 aaagcgactg ttgccagcgg cgccgtgctg cccgaactgc ggcggttcac tgagctatct
     2821 gggcgaggat accgccgaac agctggagtt gatgcgtagc gccttccggg ttatccggac
     2881 ggtacgggaa aaacatgcct gtactcagtg cgatgccatc gtgcaggcac ctgcaccttc
     2941 gcggcccatc gagcggggta tcgccggacc ggggctgctg gcccgcgtgc tgacctcgaa
     3001 gtatgcagag cacaccccgc tgtatcgcca gtcagaaata tacggccggc aaggtgtgga
     3061 gctgagccgt tcactgctgt cgggctgggt ggatgcatgc tgccggctgc tgtctccgct
     3121 ggaagaggcg cttcatggct atgtcatgac tgacggcaaa ctccatgccg atgatacccc
     3181 ggtccaggta ctgctgccgg gtaataagaa gacgaagacc aggcggttgt gggcgtatgt
     3241 tcgtgatgac cgcaatgccg ggtcagcgtt ggcacctgca gtgtggttcg cttacagccc
     3301 ggacaggaaa ggcatccatc cgcagactca tcttgcttgc ttcagcggtg tgctgcaagc
     3361 ggatgcgtac gccgggttca acgagctgta tcgcaatggt cggataacgg aagctgcctg
     3421 ctgggctcat gcccgccgaa agatccacga tgtgcacgtc cgcatcccgt cagcactgac
     3481 ggaagaagcc ctggagcaga tcggtcagtt gtacgccata gaggcggata taaggggaat
     3541 gccggcagag cagcggcttg ctgaacgtca gcgaaaaacg aaaccgctgt tgaaatccct
     3601 ggaaagctgg ttgcgtgaaa agatgaagac cctgtcgcga cactcagagt tggcgaaggc
     3661 gttcgcgtac gcacttaacc agtggccggc actgacgtac tatgcgaacg atggctgggt
     3721 ggaaatcgac aacaacatcg ctgaaaatgc cctgcgggcg gtcagtctgg gtcgtaaaaa
     3781 cttcctgttc ttcggctctg accatggtgg tgagcgggga gcgctactgt acagcctgat
     3841 cgggacgtgc aaactgaatg acgtggatcc agaaagctac cttcgccatg tgcctggcgt
     3901 catagcagac tggccggtca accgggtcag cgaactgctt ccgtggcgca tagcactgcc
     3961 agctgaataa cacatccccg tcaatacggc cctcgctgta cgcttacgac cgctcagtaa
     4021 atatcaatac agttctgacg agccgcttac aagctgtgat tcatacagac tgagcacacg
     4081 ggctgcgaca gcaacaccga tgcgttcagc aagcttcagg gcttcactgc gaaattcagg
     4141 cgaatgctat ttacggggtt ttttactggt tggtactgtt tttttgtcat atgagccacc
     4201 tctgactgag agtttactca cttagcgtgt gtccactatt gctgggtaag ctcattatcc
     4261 tgaaaaacct gcctcagtaa ggatcctctt ccccttcttt acgcgttttc agcaacttga
     4321 ggatccacgt atactgctca ggatgtggac gaacgaaatt ttcaaccact tcgtttatct
     4381 gtcgcgcaat atgagcatca ctcttctgtc gtatagcaag tggcgggctg acatgaatag
     4441 tcagatgatg ggtctttccg tcataaacag ggaaaagcgg tattatccgt gcaccactga
     4501 tcctgctgag ccgtccgata accggtaaag ttgctttata cgtcgcaaaa aaatctgcaa
     4561 actcactgaa ttccgggcca tgatcctgat caggaagata atatccccag tatccctgcc
     4621 ggactgagcg gacaaaggag gcaataccgt cattacgtgc gtgcagtttt cctccgaaac
     4681 gacgacgaac actgttccag acataatcca ccaccggatt tctctggtta tgaaacatcg
     4741 ctgccatttt tctgcccgac gcagctatca gcattgcggg aatatccact ccccaggcat
     4801 gtggcacaag aaaaatgacc ttttcattgt tctgtgccat cttctccaca atctccagac
     4861 cattccagcg tatacgatgc gaaattttat caggcccgga cagtgctaat tcagccataa
     4921 gcacaacggc catggaggct gttgcaaaca tggcatcaac gatattctcc ttttctttat
     4981 cactgtactc cggaaaacaa agcgataaat taatcaaggc ccgttgacgt gcgcttttcc
     5041 ccagacgccc aaccagcatg cccaattttc ccaacaatgg atctctcagt gcggggggga
     5101 ccattgcaaa cacacagata ataccgatac ctgtccacgt catccagtaa cgggggaagc
     5161 ataatgtaag gttaaagcgg ggaataaact cagttctgat atttttcata tttaccggta
     5221 tctgcctgct tatctcatta tgataacaga gtacagcaga aggtggtacc atccctggag
     5281 gccaccggac agcagaggaa aacacatcct cctgccttca gaacggaacc gaagataaga
     5341 aaatactatg gctcaccata cggaagagtg tcgaaatcag tgagcccatt ctttttatac
     5401 ggatttccaa tccagcgggt agtttccctg aattgcggat tcaccagaga acgggtcgga
     5461 tcgtaaccat cgtatgtcag tcccgccaaa tctgaccagg tgtggatcag ttcagccaga
     5521 ctgtatttgc ggtcaacata ctgcgaaaaa tctcgcggat gtgcagcatg ccacttttcc
     5581 gaggtccaca gcaggaacgg aacagtgtac atgggacgtg tggggttatc ttcattccgt
     5641 ccctgcgttt tatatggcgg agtatcatat acttcttcac catgatctga aaaataaagc
     5701 agaaagccgt ccggcgcagt tgctctgaag tcctttatca gactggccac cacatgatcg
     5761 ttaaacagat tggcattatc gtaatcgtta tacacttcca gctctttcgc atttaatcca
     5821 gtgggaatat gccctgtaat gccatcaaac cgcccctgac cttccggata acggtattta
     5881 tacttaatgt gcgtacccag cagatgcacg atgatcagtt tctttggtgc agggtcattc
     5941 agcacttccc ggaacggctt cagcacgtta gtgtcatatt cacgcgcact ttgtgttcgc
     6001 tgctgattca tgtagtactg cctgtccgtc tggcgcgaaa atacagtgag catggtgtta
     6061 cgggctgtga ttgtctgctg gttggtaatc cagaatgttt tatagcctgc ctgcttcatc
     6121 atgttcatca gcgacggctg cgtcagatac agatcaggat tcttttcgtt ggcgaaagta
     6181 agggcctgtt gcaatgcttc aatggtgtac ggacgcgatg ccaccacatt attaaacaca
     6241 gtaagacccg gatcggtttt acgcagtgca tccagctccg gcgtcgtttc acgtagatac
     6301 ccgtataagc tcatgcgttc gcgctgtgtc gactcaccaa tcaccaggac cagtgtgcgt
     6361 ggtctctccc ctgattcatc cctgagatta cccagtggtg gcagtgcgct attttcattc
     6421 aggaaggttg tcagtgcatt cagttgctga tggtactgat aataactgga tacaaactgc
     6481 cagggggcag caggctccat tcgggatgcc agtttgccta gagtatcatt cagcggctcc
     6541 tgtctgatta acgatttcag aacaaccgga tgcagaagca gagcataaag cagcaggaaa
     6601 gagacaattc tccgccatgg caaaggaata tatacaggac gcagacgtgt ccacagaaaa
     6661 acggacaccg cagtatatac cagcgagata agcaacagtt taaggctgaa atactggctg
     6721 aaatattcac cagcctctct ggcattcgtt tcaaacataa cgaaaagaac actttgagag
     6781 aattcatgac catagagaaa ataataacaa agtgctgcca gcgacgtgcc ccagagaatg
     6841 aaaccgacaa cagctgcaat tatttttatc cgatcaggat agagaaacac cgggatcaac
     6901 cacagacaac tgaataacag tgagtcccgt attccattcg ttccgctgta cccactgctg
     6961 aagatgatga cctgcaggag agtggaaaaa aagccaaaat aaaaaattgc ccatcccagc
     7021 gcactccagc tgaaagtagg cctgttctgt ccggtattta aatgcattga ccgtccccgt
     7081 atttaaacaa tgtgataaat tactccgtta cttgccggaa aaccgctgaa caaaattcgg
     7141 gctgaaaaga ggatccgccg ttatctgtta catttcccct tagcctgact agccagagac
     7201 acaatgatct gtgccgttct gttaatatca aaccggtact caatatcttc tctggcgctg
     7261 gctgccatca tccggaagcg ttccggtcgg gataaaaaat cgcgcagtgc gccggtccat
     7321 gcagacacat cccccacggg taacagcgtc cctgtcacat tcttctgaat gacatcaggg
     7381 atcccgcccg tctcactggc gataacgggc acgccagaga ctgacgcttc agccagtacc
     7441 ataccaaacg cttcattttc cgaaggcatg accaccacac tggcaatccg gtagaccggt
     7501 aacgctggga aaagggcacc tgccattaac acatctccgc tcattcccag gtgttctgtc
     7561 tgctgacgca gacgtgcttc gtattcttca cgcccggcgc ccaccacgag ccagcgaaat
     7621 gatttccctt ccatcttcag ctgatacaat acacgcagca taaattcatg tcctttttcg
     7681 ggacgtagca tccccacctg aacgataagc ggaacattgt ctgctgatgc agcccaggcg
     7741 tggatatgca ggggtaacgg tcgcatggct tcattatgca atgcgggcca gtcgaaaccc
     7801 ggtggaataa ccgttaccgg tgtcctgaca ccttccgcca tcagatgcgc catcatggct
     7861 gagctaggca caacaatgaa atcacacaga taattcaggg aaaacgttcg ggtcttacgg
     7921 gtgatgtagg ttttttgtct gacaatactg aagcggtgac agcatatcag acggctcagt
     7981 cctgctatat tactgtcatg gccactatgg cagatgacca gatcaggttt aaattccccg
     8041 ataatccgtc gaagtctgag gatggaagga aggtgaaggc tgttcctgaa aggaataaaa
     8101 gtgacatcat gccctctttt tctggcttcc ggagcaattt tacttttttc cctgcaggca
     8161 agtaaaacgg aatgtccctg cttttgaaga gcagtcatct gggccagtgc ctgcagctcc
     8221 tggcccccaa tatctgacga agattcagtg aataaaattt tcatcattaa ttatctggta
     8281 atcttggccc cttatgaaga gccagtactg aagaaaaaag tggcgttgta taataaaaaa
     8341 ggcgtcgttt cagccagccg gaatgttccc gctctttggt gctgatacgc cctatgcgta
     8401 atgaaccatt ttcaggacag ttcattcttc tttcggttgt atataaataa gagaaaccaa
     8461 gcttaccggc gagattaata taatcccgat tataatatcc ttcaggccag cacagatggg
     8521 aactacagaa ccccagcttt tccgtcagac attgttttcc caccagaata tcttctttca
     8581 tgagccggca ctgttcagca cgtgatacag acaacctgtc ccagcgttta tgtgaatggg
     8641 tgtgcacatg aaattcaacc agtccggaat cacgcatttc ccggacttca gaccagcgaa
     8701 gcatgacctc atctgaacgg ttatcggcaa tcaaccgttc acagtcgcgg tgagaatatt
     8761 cctgctcctg cctgctacgt acgtttccct taccaatcag ccccgtaatg agaaaaatat
     8821 gtgcatgaag gttatattcc ttcaggaccg gccatgctct gagccagtta tcaagataac
     8881 catcatcgaa tgtgagcatg acactctttc gtggaagcgt tcccccctga taaaaatatt
     8941 caagctcagc agatgttacc gtccgccagt tattatctgc gagccacttc atctgttcac
     9001 aaaatgtttc aggtgagagt gtcacaagcc ccggacaacg actgacatga tgatacatca
     9061 gtacgggaag atgccttgca ttcaacataa aaaattatcg tctgttaagc attctgaaaa
     9121 tatcctcgcg taaacaacag tgaggatcca tagatatcac acatggtgat attattgtgt
     9181 aatcccggaa tggtccggag attaccgaca acagggattt tttaatattt ttatcagtta
     9241 atcaaccaga acttaaattt cccttaacgc atatgctctt tttaatcaga ttttctgttt
     9301 ttcagaaaaa acagaatacc gcaatttcag taaacacagc gagagatatc cgcctcagta
     9361 aaaaatcaga agattatcat cctgatttat tgcataaaac cagtcgagta gcggaatttt
     9421 ctgcatccgg ataccactcc ccctgaaaaa tactcgcaca atatcgcgcc gcatgcactg
     9481 atggaccggg ccatcgtgag aatctcttgc atcgtagata ttaaccacac cagtgccccc
     9541 cagaacaaca gtaatacctg tagcagacct gccatagagg ttaaataacc attcatatcc
     9601 ctcttctacc cattcatccc gaagtttcag ccagcgacga atggactgta cctcctgttt
     9661 cagccgaggg gctgagttga caaaccgttt agagccgccg gatattcagg ttaccggcct
     9721 gcaaatcaat atcagacagt tttataccta ataactctga tgcttgtaca ccatgaagat
     9781 accccataag gagcaggcag tgattacgtt ccgggtgtgg cactttgaag acagcactgt
     9841 gcaacttatg aatctcaccc gggttcagat atttttgttg tccattacct gtccttattt
     9901 attgaaagtc gatattagtt taaaaagctg ctaatcatga caccattaca gaagtaaaat
     9961 taatttattt taaatacata aaattattgt tcatttattt ttttgcgaac attcataaac
    10021 taaaaaaaca atggataaaa ccaataacat tgcataataa tacacctccc ttataaataa
    10081 tggagaagaa aatgaaaagg cggtatatca cctatgctga acctgtttag gatgctgtgt
    10141 aaatgccttt tctccgaagt gaccgtccaa gcggtcaccg aattcgataa taaagcggct
    10201 cattgccata caccagtcct tcaacggcat cgtccatttc tgaggcgcag actggatagc
    10261 taaagtgtag cggtattagg agctgttatt tagttcgaat atcgaaaatt tttgtgtacg
    10321 ttggtcgtat caatctcttt ttatttgaat tagtttgata acagattttt gcaatctgat
    10381 aactcattta tttattgagg gataaatgca atattgtaca atatgtttcc gccattagca
    10441 aaagaatcca caaggcgctt gcactaatgc atggtaatgc ctccaattag ttgaattagg
    10501 ggtatatgaa ccgccccggg aatcctggag actaaactcc ctgagaaaga ggtaaacagg
    10561 atgactaaaa atacacgttt ttcccccgag gtccgtcaac gggcagttcg tatggttctg
    10621 gaaagtcagg gcgaatatga ctcacaatgg gcggcaattt gttccattgc cccaaagatt
    10681 ggctgtacac cagagactct gcgtgtgtgg gttcgtcagc atgagcggga taccgggagt
    10741 ggtgatggtg gactcaccac cgctgaacgt cagcgtctga aagagctgga acgtgaaaat
    10801 cgtgaactgc gccgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg
    10861 gagttcgacc gcctctggaa aaaataatgc ctctgctgga taagctgcgt gagcagtacg
    10921 gggtcggacc ggtatgcagt gaactgcata ttgccccgtc aacgtattac cactgtcagc
    10981 aacagcgaca tcatcctgat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga
    11041 gagagataca gcgcgtatac gatgaaaatc atcaggtata cggtgtgcgt aaagtctggc
    11101 gccagttgtt acgcgaaggt atcagggtgg ccagatgtac agtggcgcgc ctcatggcgg
    11161 ttatgggact tgccggtgct ctccggggta aaaaggtccg tactaccgtc agccgattta
    11221 cgggccgtaa gtaacgaagt tggatgcaaa tgtcagatcg tgtgcgcctg ttatggctcg
    11281 gctggtaaga gagccttata ggcgcatttg aaaaaactcc agctataccg gaagatattt
    11341 attattaaca ggagaggttt gcttttctgt gtaggtttag attttgtgtt tgcagggtaa
    11401 acaaaactga ttaactcgtc atttcggtaa ctgaaatagt ttgc
//
LOCUS       DAZFZC010000134        10962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFZC010000134 DAZFZC010000000
VERSION     DAZFZC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.135"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..193
                     /locus_tag="ACHKXP_004384"
     CDS             <1..193
                     /locus_tag="ACHKXP_004384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649258.1"
                     /translation="SSRFPRNVKTAAPETQRVMFFKNQDLFQAPAIRIYLFCQSSGWQ
                     VMRTQEQKEENTGKRQMQI"
     gene            290..520
                     /locus_tag="ACHKXP_004385"
     CDS             290..520
                     /locus_tag="ACHKXP_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649259.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            572..1933
                     /locus_tag="ACHKXP_004386"
     CDS             572..1933
                     /locus_tag="ACHKXP_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO7649260.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLVER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTIWRAESLD
                     LNMAKLISSHDRISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HKRQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGTVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            1981..2544
                     /locus_tag="ACHKXP_004387"
     CDS             1981..2544
                     /locus_tag="ACHKXP_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO7649261.1"
                     /translation="MYSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAGISNRDHILEPSAGTGAILRAIRDTAPVAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(2630..3037)
                     /locus_tag="ACHKXP_004388"
     CDS             complement(2630..3037)
                     /locus_tag="ACHKXP_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649262.1"
                     /translation="MTTGRDPPQRREARFAKRRCALHPGGLLPGCQERRPRRDRGARP
                     EQSLQARGFASGMEARQGGDSSAGSVHDSPPRQGDARKRHRQKKNKTDSNQSSRKKKN
                     SSSSRNIISNRQMTDLKRQKPTAEFPRNERGGA"
     gene            3350..3847
                     /locus_tag="ACHKXP_004389"
                     /pseudo
     CDS             3350..3847
                     /locus_tag="ACHKXP_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            3945..4178
                     /locus_tag="ACHKXP_004390"
     CDS             3945..4178
                     /locus_tag="ACHKXP_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7649263.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            4242..6200
                     /locus_tag="ACHKXP_004391"
     CDS             4242..6200
                     /locus_tag="ACHKXP_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7649264.1"
                     /translation="MSVTESKTKTERKSSRKPSKEQETALSALLAQTAEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRYVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPDAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            6255..6689
                     /gene="psiB"
                     /locus_tag="ACHKXP_004392"
     CDS             6255..6689
                     /gene="psiB"
                     /locus_tag="ACHKXP_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7649265.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQV"
     gene            6686..7472
                     /locus_tag="ACHKXP_004393"
                     /pseudo
     CDS             6686..7472
                     /locus_tag="ACHKXP_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            7662..7775
                     /locus_tag="ACHKXP_004394"
                     /pseudo
     CDS             7662..7775
                     /locus_tag="ACHKXP_004394"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            7720..7842
                     /locus_tag="ACHKXP_004395"
     CDS             7720..7842
                     /locus_tag="ACHKXP_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7649266.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK"
     gene            complement(8143..8440)
                     /locus_tag="ACHKXP_004396"
                     /pseudo
     CDS             complement(8143..8440)
                     /locus_tag="ACHKXP_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8741..9028
                     /locus_tag="ACHKXP_004397"
     CDS             8741..9028
                     /locus_tag="ACHKXP_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649267.1"
                     /translation="MSTRNIHVNIASYTLLVAGKKKYTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            9147..9968
                     /locus_tag="ACHKXP_004398"
     CDS             9147..9968
                     /locus_tag="ACHKXP_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7649268.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(10264..10773)
                     /locus_tag="ACHKXP_004399"
     CDS             complement(10264..10773)
                     /locus_tag="ACHKXP_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7649269.1"
                     /translation="MKKWMLAICLMFINEICLATDCFDLAGRDYKIDPDLLRAISWQE
                     SRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHMTTDPCMNIYTGAYYLAI
                     AFKKWGVTWEAVGAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRVPTTKKSL
                     SQMNSVQNN"
BASE COUNT         2555 a         2822 c         3172 g         2413 t
ORIGIN      
        1 cagcagcagg ttcccgcgaa acgtcaaaac cgcggctccc gaaactcaac gggtgatgtt
       61 ctttaaaaat caggatttat ttcaggctcc ggcaatacgc atttatttgt tctgtcagtc
      121 atccggctgg caggtaatga gaacgcagga acagaaggag gaaaacaccg gaaagcgtca
      181 gatgcagata tgaaaaaagc aggtcaggga cctgcttaat ccacgccgta gaaatcgccg
      241 caatcgattt tcacggcttc actaacctgt gtcataagga gtttcattta tgacagtaag
      301 tattgtttca ccttccgcag cggctgtaaa gccccgccgt catccccgtt tcagacgcga
      361 agacatcccc gcgccggaaa ttgaccccgt gctgaaagca ttcgggcgac acatagcccg
      421 gagttttcac cggggacgtg gtgtacacat ccccgccatg aaaaacacgg ctttcgggca
      481 ggtgctccgc acacttgaac tgaaacgcgc ttttaactga tgaggtgagc cgccccgcga
      541 ggggcggcaa cagacggaag gagaacattt tatgacatta gcagcaatca ccatgaccgc
      601 cccagaagcc gccagccctg tgcagatgta ccgcgcgacc tactcaccgg atgacaacaa
      661 actgcgcctg tatgctgcgt cacgtcttga cccggagacg tataaaaaag tgcatgatgc
      721 cggttttcgc tgggcaccaa aacaggcgct gtttgtggct ccagcctgga caccgggccg
      781 ggaagacgtg ctcctctcac ttgccggaga gattgaggat gaagacagca cgctcgctga
      841 acgtcaggaa gcacgggcgg agcggtttac cggatacagc ggaaagcggg ccagtgaatc
      901 cgcacaggca cttgatgaag tggaaagact ggccgcgatg atcccgcccg gtcagcccat
      961 tcttgtgggg catcacagcg aacgccgcgc ccgtcgtgat gcgcagcgta ttgaaaacgg
     1021 catgaaacgt gccgtgatgc tcgttgaacg tgcggaatac tgggaagagc gggcgcggtc
     1081 ggcactgctt cacgcgaagt ataaagaacg tccggacgtt cgctggcgtc gtatcaaaaa
     1141 aatcgaagct gatttgcgca aggctgaaaa gaccatcgcg cagtcgcaga aatatctgac
     1201 gatatggcgg gctgaatcgc tggatctgaa tatggcaaaa ctcatcagta gtcatgaccg
     1261 tatcagtgcc tgtttcccgc tggatacgta tccgcgcccg gcagaaaaaa gccagtatga
     1321 aggaagtcga tcgttatggt cggccctgga tgatgacatc atcaccacgg agcaggcccg
     1381 cgaaattgcg atccgctgtc ataaacggca gattcagcat cagcaacgct gggttaacca
     1441 ctatcagaac cgcctgatct atgagcgtgc catgctggac gaaagcggcg gcgtggttac
     1501 ccggacacag gattttgagc cgggcggaca ggttttcagc cggggcgagt ggctgaccat
     1561 catccgcgtg aacaaaagca acgggacggt gagttcagtc acaacgccga attacagttt
     1621 tctcgggtac agtggcacga tgaaagtgac gcccgatcgc attacggact acaaagcacc
     1681 atcggcagaa gaggctgccg tcgccagtca ggccgctaag cgtccgcctg ttgtcaacta
     1741 tccgggagaa ggtttccggg aaatgacaaa ggcacagtgg gctgccctgc cccgggactg
     1801 taaggccgtg cgcagtgtgg cagaggcaga agaccacggg gcatatcgtt accgccgcac
     1861 aatggacaat aatttccgcc tggtgaatgt gtatatcacc gacatgaaga ttacggaaat
     1921 cccacagaaa taaggtatat cctcccggga aaatccccgg gggatgacgg agataatgac
     1981 atgtacagtc agttaagaga acgtatccgg ctgatgcgcg caaggctgga taacgccgcg
     2041 ccggttgctg aaatccgggc tgaatctcag ctttttgtga ctcctgcccc ggtctgtgat
     2101 cgcctggtga cgctggcggg gatcagcaac cgtgatcaca ttctggaacc ctctgccggc
     2161 accggggcca tactgcgggc gattagggat accgcgccgg tagccatgtg tgatgcggtg
     2221 gaaatcaaca gcgggctggt ccggtatctg cgggaaaatt ttaacggtgt cagggttcag
     2281 tgtggcgact tcatggaatg gcagccggtg cagtattaca gccgggttat catgaatccg
     2341 ccgttcagcc acgggcagga tatccggcat atcctgcggg ccttttccct gttgcgtccg
     2401 ggtggcgtgc tggtcgccgt ctgtctcaac gggccacgcc agcaggagaa gctgttaccg
     2461 ttttctgacg tccgcgagga gctgccgcgc ggcacatttg cttataccga tgtcccgacg
     2521 atgattattc gtctgcgtgc ctgaagactc cgggcaggcg ggaggataaa tgctcgtctg
     2581 cccgcgccgg gaaaagaccg cgcgccggtg gcgctgaaag gggccgcgct cacgcgccgc
     2641 ccctttcatt acgcggaaat tctgctgtgg gcttctgtct tttcagatct gtcatttgtc
     2701 tgttactgat gatgttcctg ctactgctac tgtttttctt ttttctgctg ctctgattac
     2761 tgtctgtctt gttcttcttc tgcctgtgcc ttttccgggc gtccccctgg cggggcgggc
     2821 tgtcgtgaac ggagccagcg gagctgtctc cgccctggcg ggcttccatc cctgacgcaa
     2881 aacccctggc ttgcagcgac tgttccggtc gcgcgcctct gtcgcgccgt ggcctgcgct
     2941 cctggcatcc cggcaaaagg ccgccgggat gtaaggcgca acgccgtttc gcaaaccgcg
     3001 cttcacgtcg ttgcggcggg tccctgcccg ttgttatgtc cggtgccatt cacctgttta
     3061 agcccctgtc gccgggggca aagacagcca caaccgtctc cgccagtcca ggggtgaaat
     3121 gcacatcccg caaaactttt tgcccaaaag caggcaaaaa cttttgcggt ctgcccccgg
     3181 acagtcgttt cctgccgtgg cagtgatgcc ccttgcggcg aaggttttca aaaaggtgat
     3241 ggtgaatacc ggagggaaca aaaggcaggt ccccgcaaaa cgtcaaaacc gcggctcccg
     3301 aaactcaaaa gatgatgggg ctgaaaacat caacagaagg agacacatca tggcagttcg
     3361 tggcattaac aaggtcattc tcgtaggacg cctgggaaaa gatccggaag tccgttacat
     3421 ccccaacggg ggcgcagtgg caaacctgca ggtggccaca tcagaaagct ggcgtgacaa
     3481 acagacgggg gagatgcggg agcagacaga atggcatcgt gtggtgctgt tcggcaagct
     3541 cgcggaagtg gcaggtgaat atctgcgcaa gggcgcgcag gtctacatcg aaggtcagct
     3601 tcgcacccgt agctgggagg ataacggtat cacccgttac gtcaccgaaa ttcttgttaa
     3661 gaccacgggc accatgcaga tgctgggacg tgtcgcaggt gctcagactc agccggaaga
     3721 ggggcaacag ttcagcggtc agtctcagcc ggaaccacag gcggaggccg gtacgaaaaa
     3781 aggaggcgca aaaacgaaag gccgtggacg taaggccacg cagccggagt cgcagccgca
     3841 accgccggag agtgacgatt acgggttttc agatgatacc cgttctgaac gactgactgt
     3901 gacaaccgct ccgtcctgtg cggggcatca ccggagagat gaggatgagc gaatatttca
     3961 gaatacttca ggggctgcct gacggtcctt ttacccgcaa acatgccgaa gctgttgccg
     4021 cacagtaccg gaacgtcttt atcgaggatg accacggcga acagtttcgc ctggttgtcc
     4081 gtaataacgg tgcaatggtc tggcgtacct ggaattttga ggacggtgcc gggtactgga
     4141 tgaaccatgc catccgtgat ttcggcattc ttaagtaaga gaggtgccgg acgggaatgc
     4201 gtccggcaga catacaacca tcccggtcaa aggagaaata tatgtcggtt acagagtcta
     4261 agacaaaaac ggagcgtaaa tccagccgta aaccgtcaaa agagcaggag actgccctgt
     4321 ctgccctgct ggcgcagacg gcggaagtga gcgtgccgct ggcctcactg attaaatcac
     4381 cactgaatgt gcgcacggtg ccgtattctg cggagtctgt cagcgaactg gcggagtcca
     4441 ttaagggcgt tggcctgttg cagaatctgg tcgtgcatac cctgcctggt gaccgttacg
     4501 gtgtcgccgc aggtggtcgc cgactggctg cactcaacat gctggcagag cgtggcatca
     4561 ttccggctga ctggcctgtc cgtgtgaagg tcattccgca ggagctggcg acggcggcat
     4621 caatgactga aaacggtcat cgtcgggata tgcaccctgc tgaacagatt gccggattcc
     4681 gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg tgatttgctg ggctattcac
     4741 cccgctatgt ccagcgaatg ctgaaactgg ctgaccttgc gcctgtcatc ctcgatgcgc
     4801 tggcagaaga ccgcatcact accgaacact gtcaggcgct ggcgctggag aacgacaccg
     4861 cgcgtcaggt acaggtgttt gaagccgcct gtcagtcggg atggggcgga aaaccggatg
     4921 tgcgggttat ccgcaatctg attacggaaa gtgaagtggc ggtggcgggg aacagtaaat
     4981 tccgcttcgt gggggctgat gctttctcac ctgacgaact gcgtactgat ttgttcagcg
     5041 atgacgggga cggttatgtc gaccgcgtgg cgctcgatgc tgccctgctg gaaaaactcc
     5101 aggctgtcgc tgaacacctt cgggaagccg aaggctggga atggtgcgcc ggacgcatgg
     5161 agcctgtcgg tgagtgccgt gaggatgccg gaacataccg ctgtttgccg gagccggacg
     5221 cggtgctgac ggaggcggaa gaagaacgcc tgaacgaact gatgacgcgt tacgacgcgc
     5281 tggaaaacca gtgtgaggaa tccgacctgc tggaagcaga aatgaagctg atgcgctgca
     5341 tggcgaaggt cagagcgtgg acgccggaga tgcgtaccgg aagcggtgtg gtggtgtcct
     5401 ggcgttacgg taatgtgtgt gtccagcgtg gtgtgcagtt gcgcagcgag gatgacgcgg
     5461 ctgacgacgc tgaccgcacg gaacaggtgc aggagaaagc atcagtggag gaaatcagtc
     5521 tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggcg gcactcatgc
     5581 agcaaccgga caaatccctg gcactgctgg catggacgct ctgcctgaat gtgtttggca
     5641 gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat tcgctgacca
     5701 gcgatgcgcc gtcagggaag gaaggtgccg cgttcatggc gatgatggca gaaaaagccc
     5761 gtcttgcggc cctgttaccg gagggatggt cacgggacat gacgacgttc ctgtcactca
     5821 gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc gtgcagcatc cacggcgttc
     5881 agacccgcga gtgtggtcac acgtcacgca gcccgctgga cacgctggag agcgccatcg
     5941 gttttcacat gcgtgactgg tggcagccga caaaagcaaa cttcttcgga cacctgaaaa
     6001 agccgcagat tatcgcagct ctgaatgatg ccggactgtc cggtgccgca cgggatgcag
     6061 agaagatgaa gaaaggtgat gcggctgaac atgcagagca ccatatgaaa gacaaccgct
     6121 gggtgcctgg ctggatgtgt gcaccacatc cacagacgga taccactgaa cgcaccgata
     6181 acctggctga tgccgcctga tgaacaacca caccgccccg ccggagacgg ggcagcagca
     6241 agggagatac cgtgatgaaa actgaactga ccctgaatgt attacagacc atgaacgcac
     6301 aggaatatga agatatcagg gctgccggaa gtgatgaacg ccgtgagctg acacacgccg
     6361 tgatgcggga actggatgcg ccggacaact ggacgatgaa tggcgagtac ggcagcgagt
     6421 tcggcggctt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt tttcacctgg
     6481 cgttatgttc accgggtgat gtctcccagg tctgggtgct ggttctggtg aatgccggtg
     6541 gtgagccgtt tgcggtggtt caggtacagc ggcggtttgc accggaagca gtcagccatt
     6601 cactggcact ggcggcgtca ctggatgcgc aggggtacag cgttaacgac atcatccata
     6661 tcctgatggc ggaaggaggt caggtatgaa cgcacgttca cgggcactgg ttcccctcag
     6721 cacagagcaa caggccgcat ggcgggcggt ggcggagacg gaaaaacgcc gccatcaggg
     6781 aaacacgctg gcggaatatc cgtatgcggg agcatttttc cgctgtctga acggaagccg
     6841 ccggatttcg ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag aagaactgca
     6901 cggcaaccgt cttcagtggc tgtgtgccat cgatgtgctg attgaaacac agggcgaggt
     6961 ctgcctcctt ccccttcccg gcgatgcagc agaacgcttg ttcccgtcgg tgcggtttcg
     7021 tgtcagggaa cgcagtcgcc ataaatccgc gctggtcatg cagaagtaca gccgccagca
     7081 agcgagggag gcagagcaga aagcccgtgc gtaccaggtg ctggttgctc aggctgaaat
     7141 cgaactggca tttcattcac cggaaacagt cggaagctgg catgcacgct ggagtgaccg
     7201 ggtggcagag catgacctgg aacccctgtt ctggcagtgg ggcgagcgtt ttccgtcact
     7261 ggccggaatg gaacgctggc agtggcagga tatgccattc tggcaggtta tcgcggaagc
     7321 cagtctggca gccagagagg ccggtcatgc tgtccgtgag atggagcggt ggatggtgcc
     7381 gaacaaactc cgggaggcag cgtgatgcgg caataatcac acggattacc cgtgaactgg
     7441 ttgaacgacc agattatttt cagggaaagt gagtgtggtc agcgtgcagg gatatgagct
     7501 atgatgtgcc cggcgcttga ggctttctgc ctcatgacgt gaaggtggtt tgttaccgtg
     7561 ttgtgtggca gaaggcagaa agccccgtag ttaatttttc attaacccac gaggccccct
     7621 gtatgtctga tcaacaacag tatggcctct taccgtgctt aatgcaagga ggagtaaacc
     7681 atgaaactac cgggaaacgc ccttatctgg tgcgtattaa tcgtgtgctg cacgttgtta
     7741 atattcacac tcctgacccg gaatcgtctg tgcgaagtcc ggctgaagga cggatacagg
     7801 gaggttacgg caagtttggc ttacgaatcc agcggtaagt agcaacctgg aggcgggcgc
     7861 aggcccgcct tttcagggct gatgctggtc tgactgcact gaagcgcctt tataaagggg
     7921 ctgctggttc gccggtggcc cctttctcct tgctggcatt gtgcgggcat gaacaaccag
     7981 actgccagga aggccgtttc cctccgggaa cggggatgaa cgtgcgcctg cggcgcccgt
     8041 tcttttcccc cgccttctct gtttatgacc tgtcagaaat cacatcacct tccctttgat
     8101 tattctcttt ttcctcctgt tctgactctg attactgctg ccctgattat tgtctgtttt
     8161 gttgttctcc tgcttatgcc ttttccgggc gtccccctgg cggggcgggc tgtcgtgaac
     8221 ggagccagcg gagctgtctc cgccctggcg ggcttccatc cctgacgcga aaccccgggc
     8281 ttgcagcgac cgttccggtc gcgccgcctc tgtcgcgccg tggcctgcgc tcccggcatc
     8341 ccggcaaaag gccgccggga tgtaaggggc aacgccgttt cgcaaaccgc gcttcacgtc
     8401 gttgcggcgg gttcctgccc gttgttgtgt ccggtgccat tcacctgttt aagcccctgt
     8461 cgccgggagc tgatacagcc acaacctgcc gcactgactg tccagggtaa atgaacagcc
     8521 cacaaaattt tttacccaaa acccggtaaa aaattttgcg ttctgcccag gacaggtgcg
     8581 tcaggccgtg gcagtgatgc cccttgcggc gaaggttctc aaacaggtga tggtgaatac
     8641 cggagggaac aaacggcagg tccccgcaaa acgtcaaaac cgcggctccc gaaactcaac
     8701 agatgatggg ggctgaaaac cagagagagg agaaagcagg atgagtaccc gaaatatcca
     8761 cgttaacatc gcgtcgtaca cgcttcttgt tgccgggaag aaaaaataca ccggggaaga
     8821 gtgggacgtc ctggaattca gcagcctgac tgagctgaaa aaatatcgca aaagccaccc
     8881 ggaaaagatg gccttcagct atagctatgc gctcagccgg ggtgtggata cgcagttccg
     8941 ccatatcaac attgcggaag ccgatcactt caaacagttc ctgcgtcaga ttaagcgtac
     9001 cggcctcgat atccgggcta tctgctgacc ggatgtgatg aatatccctc ctgattgttg
     9061 caatcttgtt aatggagatc gggagggatg atattaagcg gaaaaatttt ttattaaatt
     9121 tattctttca taaaggagta gctgttatgc gattagcttc ccgttttggt cggtataatt
     9181 ccatccgccg tgaacgtcct ttaacggatg atgaattaat gcagtttgtg ccttcggtgt
     9241 tttccggtga taaacatgag tcccggagtg aacgttatac gtatattcca acaatcaata
     9301 tcatcaataa gttacgtgat gaaggtttcc agccattctt tgcctgtcag agtcgggttc
     9361 gtgatttggg acgtcgcgaa tacagtaaac atatgttacg tcttcgcagg gaagggcata
     9421 ttaacgggca ggaagttcct gaaattatcc tgcttaattc acatgatggt tcatccagtt
     9481 atcagatgat ccccggaatt tttcgttttg tctgcacaaa tggcctggtg tgcggaaata
     9541 attttggcga aatccgcgtt ccacataaag gtgatattgt cgggcaggtt atcgagggag
     9601 cgtatgaagt gctcggtgtc tttgataagg tcactgataa tatggaggcg atgaaagaaa
     9661 ttcatcttaa cagtgacgag caacatttat ttggcagagc tgcactgatg gtcaggtatg
     9721 aagatgaaaa taaaacgccg gtgacgcctg aacagataat tactccccgt cgtcgggagg
     9781 ataaacagaa cgatctctgg acaacctggc agcgggttca ggagaatatg ataaaaggtg
     9841 gattatcggg gcgaagtgcc tccgggaaaa ataccaggac aagagccatt acaggtattg
     9901 atggtgatat aagaatcaac aaagcattat ggatgattgc cgaacagttc agggagtgga
     9961 agtcgtgata atataggtaa agccccgaaa aattttcggg gctttactta tattgtcatc
    10021 cttcggcatt gctatgtcgt ctgatatgcc tcaatttcat caactggctt ttcggtagtc
    10081 tgtaaatatt gtgcccgcga aaaatggtct ggcaggaggc tacgcagtta ccggatatat
    10141 ataatatggg ttcccgcttt gcggggcttc ggccagtcag cattatcact catatgatat
    10201 ttttgtgtgt ggccttccat gccgctgtcg cggcataacc ggtatttgac aatgtataca
    10261 atattaattg ttctgcacac tgttcatttg ggaaagtgat ttcttcgtgg tcggaacccg
    10321 gataccttta ctgcttttta tcccggtata aatccggtaa acatctgatg cgtaggcaag
    10381 acgcctctgg ttctggcgtt cggttttcct gaatccggca ttgtatgcac caacggcctc
    10441 ccaggtgacg ccccattttt taaatgctat tgccagataa taagcaccgg tataaatgtt
    10501 catgcaggga tctgttgtca tatgttccgg cttaattccg tagcgggcca gttcgttaaa
    10561 atgctgggaa tctacctgca tcagtccgct gccatatccc gttaccggat taataccgat
    10621 ggcattaacc cggtaacggg attcttgcca tgatattgct ctcagtaaat caggatctat
    10681 tttgtaatcc ctgcctgcaa gatcaaagca atcagtggcc agacagatct catttataaa
    10741 catcaggcag atggctaaca tccatttttt catttttcca cctctggtga ctttatccgt
    10801 aaataattta actcactcca caaaaaggct caacaggttg gtggttctca ccaccaaaag
    10861 caccacaccc cacgcaaaaa caagtttttg ctgatttttc tttataaata gagtgttatg
    10921 aaaaattagt ttctcttact ctctttatga tatttaaaaa ag
//
LOCUS       DAZFZC010000135        10703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFZC010000135 DAZFZC010000000
VERSION     DAZFZC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.136"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            48..1190
                     /gene="yghO"
                     /locus_tag="ACHKXP_004400"
     CDS             48..1190
                     /gene="yghO"
                     /locus_tag="ACHKXP_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO7649270.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1197..1961)
                     /gene="glcC"
                     /locus_tag="ACHKXP_004401"
     CDS             complement(1197..1961)
                     /gene="glcC"
                     /locus_tag="ACHKXP_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO7649271.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2217..3716
                     /gene="glcD"
                     /locus_tag="ACHKXP_004402"
     CDS             2217..3716
                     /gene="glcD"
                     /locus_tag="ACHKXP_004402"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO7649272.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3716..4768
                     /gene="glcE"
                     /locus_tag="ACHKXP_004403"
     CDS             3716..4768
                     /gene="glcE"
                     /locus_tag="ACHKXP_004403"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO7649273.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARVGTPLVTIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMVGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4779..6002
                     /gene="glcF"
                     /locus_tag="ACHKXP_004404"
     CDS             4779..6002
                     /gene="glcF"
                     /locus_tag="ACHKXP_004404"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO7649274.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6007..6411
                     /gene="glcG"
                     /locus_tag="ACHKXP_004405"
     CDS             6007..6411
                     /gene="glcG"
                     /locus_tag="ACHKXP_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO7649275.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6433..8604
                     /gene="glcB"
                     /locus_tag="ACHKXP_004406"
     CDS             6433..8604
                     /gene="glcB"
                     /locus_tag="ACHKXP_004406"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO7649276.1"
                     /translation="MSQTITQSRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8959..10641
                     /gene="glcA"
                     /locus_tag="ACHKXP_004407"
     CDS             8959..10641
                     /gene="glcA"
                     /locus_tag="ACHKXP_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO7649277.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2487 a         2709 c         3014 g         2493 t
ORIGIN      
        1 caacctgttt gtcaccgtta attatttgtt acccacagaa cagaactatg gaatgcgatc
       61 tgctgatgat taaaattgaa aaagtaatta ataaaaatga ccttaaggca tttatcgctt
      121 ttccgtcatc actttatccc gacgatccaa actggatacc ccctttattc attgaacgca
      181 acgagcattt gtctgcgaaa aatccgggga cggaccatat catctggcag gcgtgggtgg
      241 caaaaaaagc ggggcagata gttgggcgta ttaccgcgca aatagatacc ttgcaccgcg
      301 agcgttacgg caaagatacc ggtcatttcg gcatgattga cgccattgat gatccgcagg
      361 tttttgccgc gttgtttggc gcagcggaag cgtggttgaa gtcacaaggt gcaagtaaga
      421 tcagcggtcc tttcagcctg aatatcaatc aggaaagcgg attactgatt gaaggttttg
      481 acacaccacc ctgtgcgatg atgccacacg gcaaaccgtg gtatgccgcg catattgaac
      541 aactgggtta tcacaaaggc attgatttac tggcgtggtg gatgcagcga accgatctca
      601 ctttctctcc ggcgctaaaa aaactgatgg atcaggtgcg caaaaaggtg accattcgct
      661 gcatcaatcg tcagcggttt gccgaagaga tgcagatcct gcgtgagatt ttcaactctg
      721 gctggcagca caactgggga tttgtgccgt ttaccgaaca tgaattcgcg accatgggcg
      781 atcaacttaa atatctggtg ccggacgata tgatctatat cgctgagatt gattctgcac
      841 cctgcgcgtt tattgtcggc ttgccgaaca tcaacgaggc gattgccgat ctgaacggat
      901 cgctctttcc cttcggctgg gcaaaattgc tgtggcgttt gaaagtcagc ggtgtgcgaa
      961 ctgcgcgagt accgctgatg ggcgtgcgcg acgagtatca gttcagccgc atcggcccgg
     1021 tgattgcgct gttattgatt gaagccttac gcgatccgtt tgcccgccgg aagatcgatg
     1081 cgctggagat gtcatggatc ctcgaaacca atacaggcat gaataacatg ctggaacgca
     1141 ttggggcgga gccgtacaag cgttaccgat tgtatgaaaa gcagatttaa taccagctaa
     1201 ctcaggttca tctccagcgg caccgccgag cgaatcaaat ggtggccttc cagctcgata
     1261 tcgtggagat tctttttcac ggtccgcaca tgatcgcgtg ctgcgcgctg ggcgacgtgc
     1321 ggcagccgct gcaacaccgc gttgtagatc cgcgcatgct ggcgatcgat ctgctttttt
     1381 tgctgtggtc gatggtagag attatttacg ctggcaaaca ctgaattaaa catcagatcg
     1441 gtcaatgatt gcagcgtaaa caccagcacc tgattgtgag aagcctgaca aatggcgaga
     1501 tggaaagcgt gatccaactg cgcatgttcg atcagcgaaa tctctttgtt gttctcactg
     1561 gcggcgagca ttttttcata acagcgggtt atcacaacaa aatcagcctg cgttcccagc
     1621 gttgccgcca gccttgccga ttcgccctcc agtaatgcgc gaacgtcgag cagatcgtac
     1681 agcgttcgcg gctgcgtact gaacagatgg atcagcgggc tggtgtcctg cacccgatta
     1741 agccgtgcga cacgagaatc gcgaccctgc gccgtttcaa taatcccgcg cccgcgcagc
     1801 acggtcagcc cttcacgcag tgcggagcgt gagaagccga gcttttcaca cagtcgacgt
     1861 tccgagggaa gcggctgacc gaccttcagt acgccgtcga taattaaccg ttcgatactc
     1921 tctgcaacca cttcgcaaat agggcgacgt tcatctttca ttcccggacc tcgtgcacag
     1981 gtaggaccaa tttttctgtt cttaactcgc aaaacacgca catcacgtaa gtgattatgt
     2041 tacatcaatt taacattgag ttaaccaaga caaggtcaca gagctggaaa aaaaatggtc
     2101 tgaccggtag ctaaagagat agacgaaaac gaaaagcccg cttaataact gttcacagaa
     2161 gcagcgcgca aaaatcagct gccacacaac acaacacaac aaagcgaagc ctactcatga
     2221 gcatcttgta cgaagagcgt cttgatggcg ctttacccga tgtcgaccgc acatcggtac
     2281 tgatggcact gcgtgagcat gtccctggac ttgagatcct gcataccgat gaggagatca
     2341 ttccttacga gtgtgacggg ttgagcgcgt atcgcacgcg tccattactg gttgttctgc
     2401 ctaagcaaat ggaacaggtg acagcgattc tggctgtctg ccatcgcctg cgtgtaccgg
     2461 tggtgacccg tggtgcaggc accgggcttt ctggtggcgc gctgccgctg gaaaaaggtg
     2521 tgttgttggt gatggcgcgc tttaaagaga tcctcgacat taaccccgtt ggtcgccgcg
     2581 cgcgcgtgca gccaggcgtg cgtaacctgg cgatctccca ggccgttgca ccgcataatc
     2641 tctactacgc accggaccct tcctcacaaa tcgcctgttc cattggcggc aatgtggctg
     2701 aaaatgccgg cggcgtccac tgcctgaaat atggtctgac cgtacataac ctgctgaaaa
     2761 ttgaagtgca aacgctggac ggcgaggcac tgacgcttgg atcggacgcg ctggattcac
     2821 ctggttttga cctgctggcg ctgttcaccg gatcggaagg tatgctcggc gtgaccaccg
     2881 aagtgacggt aaaactgctg ccgaagccgc ccgtggcgcg ggttctgtta gccagctttg
     2941 actcggtaga aaaagccgga cttgcggttg gtgacatcat cgccaatggc attatccccg
     3001 gcgggctgga gatgatggat aacctgtcga tccgcgcggc ggaagatttt attcatgccg
     3061 gttatcccgt cgacgccgaa gcgattttgt tatgcgagct ggacggcgtg gagtctgacg
     3121 tacaggaaga ctgcgagcgg gttaacgaca tcttgttgaa agcgggcgcg actgacgtcc
     3181 gtctggcaca ggacgaagca gagcgcgtac gtttctgggc cggtcgcaaa aatgcgttcc
     3241 cggcggtagg acgtatctcc ccggattact actgcatgga tggcaccatc ccgcgtcgcg
     3301 ccctgcctgg cgtactggaa ggcattgccc gtttatcgca gcaatatgat ttacgtgttg
     3361 ccaacgtctt tcatgccgga gatggcaaca tgcacccgtt aatccttttc gatgccaacg
     3421 aacccggtga atttgcccgc gcggaagagc tgggcgggaa gatcctcgaa ctctgcgttg
     3481 aagttggcgg cagcatcagt ggcgaacatg gcatcgggcg agaaaaaatc aatcaaatgt
     3541 gcgcccagtt caacagcgat gaaatcacga ccttccatgc ggtcaaggcg gcgtttgacc
     3601 ccgatggttt gctgaaccct gggaaaaaca ttcccacgct acaccgctgt gctgaatttg
     3661 gtgccatgca tgtgcatcac ggtcatttac ctttccctga actggagcgt ttctgatgct
     3721 acgcgagtgt gattacagcc aggcgctgct ggagcaggtg aatcaggcga ttagcgataa
     3781 aacgccgctg gtgattcagg gcagcaatag caaagccttt ttaggtcgcc ctgtcaccgg
     3841 gcaaacgctg gatgttcgtt gtcatcgcgg cattgttaat tacgacccga ccgagctggt
     3901 gataaccgcg cgtgtcggaa cgccgctggt gacaattgaa gcggcgctgg aaagcgcggg
     3961 gcaaatgctc ccctgtgagc cgccgcatta tggtgaagaa gccacctggg gcgggatggt
     4021 cgcctgcggg ctggcggggc cgcgtcgccc gtggagcggt tcggtccgcg attttgtcct
     4081 cggcacgcgc atcattaccg gcgctggaaa acatctgcgt tttggtggcg aagtgatgaa
     4141 aaacgttgcc ggatacgatc tctcacggtt aatggtcgga agctacggtt gtcttggcgt
     4201 gctcactgaa atctcaatga aagtgttacc gcgaccgcgc gcctccctga gcctgcgtcg
     4261 ggaaatcagc ctgcaagaag ccatgagtga aatcgccgag tggcaactcc agccattacc
     4321 cattagtggc ttatgttact tcgacaatgc gttgtggatc cgccttgagg gcggcgaagg
     4381 atcggtaaaa gcagcgcgtg aactgctggg tggcgaagag gttgccggtc agttctggca
     4441 gcaattgcgt gaacaacaac tgccgttctt ctcgttacca ggtaccttat ggcgcatttc
     4501 attacccagt gatgcgccga tgatggattt acccggcgag caactgatcg actggggcgg
     4561 ggcgttacgc tggctgaaat cgacagccga ggacaatcaa atccatcgca tcgcccgcaa
     4621 cgctggcggt catgcgaccc gctttagtgc cggagatggt ggctttgccc cgctatcggc
     4681 tcctttattc cgctatcacc agcagcttaa acagcagctc gacccttgcg gcgtgtttaa
     4741 ccccggtcgc atgtacgcgg aactttgagg agcaggctat gcaaacccaa ttaactgaag
     4801 agatgcggca gaacgcgcgc gcgctggaag ccgacagcat cctgcgcgcc tgtgttcact
     4861 gcggattttg taccgcaacc tgcccaacct atcagcttct gggcgatgaa ctggacgggc
     4921 cgcgcgggcg catctatctg attaaacagg tgctggaagg caacgaagtc acgcttaaaa
     4981 cacaggagca tctcgatcgc tgcctcactt gccgtaattg tgaaaccacc tgtccttctg
     5041 gtgtgcgcta tcacaatttg ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac
     5101 gcccactgcc ggagcgaata ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg
     5161 tcttccgtgc gctgacgcag gtagggctgg tgctgcgacc gtttttaccg gaacaggtca
     5221 gagcaaaact gcctgctgaa acggtgaaag ctaaaccgcg tccgccgctg cgccataagc
     5281 gtcgggtttt aatgttggaa ggctgcgccc agcctacgct ttcgcccaac accaacgcgg
     5341 caactgcgcg agtgctggat cgtctgggga tcagcgtcat gccagctaac gaagcaggct
     5401 gttgtggcgc ggtggactat catcttaatg cgcaggagaa agggctggca cgggcgcgca
     5461 ataatattga tgcctggtgg cccgcgattg aagcaggtgc cgaggcaatt ttgcaaaccg
     5521 ccagcggctg cggcgcgttt gtcaaagagt atgggcagat gctgaaaaac gatgcgttat
     5581 atgccgataa agcgcgtcag gtcagtgaac tggcggtcga tttagtcgaa cttctgcgcg
     5641 aggaaccgct ggaaaaactg gcaattcgcg gcgataaaaa gctggccttc cactgtccgt
     5701 gtaccctaca acatgcgcaa aagctgaacg gcgaagtgga aaaagtgttg cttcgtcttg
     5761 gatttacctt aacggacgtt cccgacagcc atctgtgctg cggttcagcg ggaacatatg
     5821 cgttaacgca tcccgatctg gcacgccagc tgcgggataa caaaatgaat gcgctggaaa
     5881 gcggcaaacc ggaaatgatc gtcaccgcca acattggttg ccagacgcat ctggcgagcg
     5941 ccggtcgtac ctctgtgcgt cactggattg aaattgtaga acaagccctt gaaaaggaat
     6001 aacaaaatga aaactaaagt cattcttagc cagcaaatgg cgagtgcaat tattgccgca
     6061 ggtcaggaag aggcgcagaa aaataactgg tctgtttcca ttgctgttgc cgatgacggc
     6121 ggtcatctgc tggcgttaag tcgcatggac gattgcgcgc cgattgcggc ttatatctcc
     6181 caggagaaag cgcgtaccgc cgcgctgggg cgtcgtgaaa ctaagggcta tgaagagatg
     6241 gtgaacaacg gacgtaccgc gttcgtgact gcgccgttat taacgtcgct ggaaggcggc
     6301 gtaccggttg ttgtggatgg gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga
     6361 gcacaggatg cgcaggtcgc gaaagcggca gcagcggtgt tggcgaaata agcgaaaacg
     6421 aggagataaa caatgagtca aaccataacc cagagccgtt tacgcattga cgccaatttt
     6481 aaacgttttg tggatgaaga agttttaccg ggaacagggc tggacgctgc ggcgttctgg
     6541 cgcaattttg atgagatcgt tcatgatctg gcaccagaaa atcgtcagtt gctggcagaa
     6601 cgcgatcgca ttcaggcagc gcttgatgag tggcatcgca gcaatccggg gccggtaaaa
     6661 gataaagcgg cctataaatc tttcctgcgt gaactgggct acctggtgcc gcaaccggag
     6721 cgcgtgacgg tggaaaccac gggcattgac agcgaaatca ccagccaggc ggggccgcag
     6781 ctggtggttc cggcaatgaa cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc
     6841 tcactgtacg atgcgttata cggcagcgac atcatcccgc aggaaggggc gatggtcagc
     6901 ggctacgatc cgcaacgcgg tgagcaggtt atcgcctggg ttcggcgttt cctcgatgaa
     6961 tctctaccgc tggaaaacgg cagctatcag gatgtggtgg cgtttaaggt ggttgataaa
     7021 caattacgca tccagttgaa aaatggtaaa gaaaccacgt tacgtactcc agcacagttt
     7081 gtcggttacc gtggcgatgc cgctgcgccg acctgcattt tgctgaaaaa taacggcctg
     7141 catattgagc tgcaaatcga tgccaatggg cggattggca aagacgatcc ggcgcacatc
     7201 aacgatgtta tcgtcgaagc tgctatcagt accattctcg actgcgaaga ttcggtcgcg
     7261 gcggttgatg cggaagataa aatcctgctg taccgcaacc tgctgggcct gatgcagggg
     7321 actctgcaag agaaaatgga gaaaaacggt cggcaaatcg tgcgtaaact gaatgacgat
     7381 cgtcattaca ccgccgccga tggctctgaa atttctctgc acggacgctc gctgctgttt
     7441 atccgcaacg tgggtcattt gatgaccatt cctgtgattt gggacagcga aggcaatgaa
     7501 atcccggaag gcattcttga tggcgtcatg actggcgcga ttgccctcta tgatttaaaa
     7561 gtgcagaaaa actcgcgcac tggcagcgtc tatattgtga aaccgaaaat gcacggtccg
     7621 caggaagtgg cgttcgccaa caaactgttt acccgcattg agacaatgct cggtatggca
     7681 ccgaataccc tgaaaatggg cattatggat gaagaacgtc ggacctcgct gaacttgcgt
     7741 agctgtatcg ctcaggcgcg caaccgcgtg gcgttcatca ataccggttt cctcgaccgt
     7801 accggcgatg aaatgcattc ggtgatggaa gctggcccga tgctgcgtaa aaatcagatg
     7861 aaatcgacgc cttggatcaa agcctacgag cgtaataacg tgctttccgg tctgttctgt
     7921 gggctgcgcg gtaaagcgca aattggtaaa ggcatgtggg caatgccgga cctgatggca
     7981 gacatgtaca gccagaaggg cgaccaactg cgtgccgggg caaacacagc ctgggttccg
     8041 tcaccaaccg ctgctacgct ccatgcgctg cactaccacc aaaccaacgt acagagcgta
     8101 caagccaaca ttgcccagac cgagttcaat gctgaatttg aaccgctgct ggacgatctg
     8161 ctgactattc cggttgctga aaacgctaac tggtcggcgc aagagatcca acaagagctg
     8221 gataacaacg tgcaggggat tctggggtac gtggtgcgct gggtggagca ggggattggt
     8281 tgttcaaaag tgccggatat tcacaatgtg gcgttgatgg aagaccgcgc aacgctgcgt
     8341 atctccagcc agcatatcgc caactggtta cgtcacggta ttctgaccaa agaacaggtg
     8401 caggcgtcgc tggagaatat ggcgaaagtg gttgatcagc aaaacgctgg cgatccggct
     8461 tatcgtccga tggcggggaa tttcgctaac tcgtgtgctt ttaaagctgc cagcgattta
     8521 atcttcctcg gcgtgaaaca gccaaacggc tataccgaac cgttattaca cgcctggcgt
     8581 ttacgcgaaa aagaaagtca ttaatagcgt caaaaaagcc ccggcaagtt ttattgtcgg
     8641 ggctatgata gtaactgcgg tagtacattt atcccggaaa taaccggagc gattatcagg
     8701 gtttaacaat attaaatacc cgtagcgcat tactagcggg cctaaaacaa caatatggac
     8761 atcttaacga tactctgcgt ctgcgtgtgc ggaacagaaa cccttacccg atatacttgt
     8821 tatgccagag gtagcaattg cgttagcggc gtactgttgc tgtaaagtat aacgcctata
     8881 ttttacagtt cagtcctctt ttgcttatta caaccttaag tatatcaagc atataaagat
     8941 aataagagac tgaacaatat ggttacctgg acccaaatgt atatgccgat gggaggactg
     9001 gggctatccg ctctggtcgc cctgatcccg ataatattct tcttcgttgc actcgcggta
     9061 ttacgtctga aaggacatgt cgctggagca ataaccctta tattatctat cctgattgca
     9121 atattcgcct ttaaaatgcc gattgatatg gcatttgctg ctgcgggcta tggctttatt
     9181 tatggattat ggccaattgc gtggattatt gtcgcggcgg tgttcctgta taaattaacc
     9241 gttgccagcg ggcagttcga tattatccgc agctcggtta tctccatcac cgacgatcag
     9301 cgtttgcagg tgttactgat tggtttctcc tttggtgcgt tgctggaagg agcggctggc
     9361 tttggtgcgc cggtggcgat taccggtgcg ctgctggtgg gcctgggctt caaaccgtta
     9421 tacgcggcgg ggctgtgtct gattgccaat actgcgccgg tggcgtttgg tgcgttgggc
     9481 gtgccgattc tggtcgccgg tcaggtaacg ggaatcgatc cgttccacat tggcgcaatg
     9541 gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt tctggctggt agcaatgatg
     9601 gacggctgga aaggggtgaa agagacgtgg ccagcggcgc tggttgctgg gggaagcttc
     9661 gctgtcactc agttctttac ctctaactat attggtccgg aactgccgga tattacttcg
     9721 gcgctggtga gtatcgtctc actcgcttta ttccttaaag tctggcggcc gaaaaatacc
     9781 gaaacggcaa tcagcatggg acaatccgca ggtgcgatgg tggtaaataa gccatcttct
     9841 ggcggtcccg tgccttcaga atatagtctg gggcaaatca ttcgtgcgtg gtcaccgttt
     9901 ttaatcttaa cggtgctggt caccatctgg accatgaagc cgtttaaagc gttatttgct
     9961 ccgggcggcg cgttttattc actggtgatt aatttccaga tccctcattt gcatcaacaa
    10021 gtgttgaaag cggcacccat tgtcgcccaa ccaacgccaa tggatgcggt gtttaaattc
    10081 gaccccctct cggctggcgg caccgctatt tttattgcgg cgattatctc tatcttcatc
    10141 ctcggtgtgg ggatcaagaa aggtattggc gtctttgccg aaacgctaat tagtctgaag
    10201 tggccgatat tgtcgattgg catggtgctg gcgttcgcct tcgtgaccaa ctattccggc
    10261 atgtccacca cgctggcgct ggtactggcg ggtactggtg tgatgttccc gttcttttcg
    10321 ccattcctcg gctggctggg ggtattcctt accggctcgg acacttcctc gaacgctctg
    10381 tttggttcac tgcaatcgac cacggcgcag caaatcaacg tctctgacac cctgctggtg
    10441 gcagcaaaca ccagcggcgg cgtaactggc aagatgatct ccccgcaatc tatcgccgtg
    10501 gcctgcgccg cgacgggcat ggtgggccga gaatctgaac tgttccgcta caccgtgaag
    10561 cacagtctga tttttgccag cgttatcggc attatcaccc tgctgcaggc gtatgtgttt
    10621 accgggatgt tagtctcgta aatcaataaa gccggatgat cgttaatatc atccggcttc
    10681 atttacatta ttctgtctta tta
//
LOCUS       DAZFZC010000136        10664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFZC010000136 DAZFZC010000000
VERSION     DAZFZC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.137"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(104..799)
                     /gene="aqpZ"
                     /locus_tag="ACHKXP_004408"
     CDS             complement(104..799)
                     /gene="aqpZ"
                     /locus_tag="ACHKXP_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO7649278.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1224..2882
                     /gene="ybjD"
                     /locus_tag="ACHKXP_004409"
     CDS             1224..2882
                     /gene="ybjD"
                     /locus_tag="ACHKXP_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO7649279.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRMAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2879..3835)
                     /gene="ybjX"
                     /locus_tag="ACHKXP_004410"
     CDS             complement(2879..3835)
                     /gene="ybjX"
                     /locus_tag="ACHKXP_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO7649280.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRECMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3986..5101
                     /gene="macA"
                     /locus_tag="ACHKXP_004411"
     CDS             3986..5101
                     /gene="macA"
                     /locus_tag="ACHKXP_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO7649281.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5098..7044
                     /gene="macB"
                     /locus_tag="ACHKXP_004412"
     CDS             5098..7044
                     /gene="macB"
                     /locus_tag="ACHKXP_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO7649282.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7117..7341)
                     /gene="cspD"
                     /locus_tag="ACHKXP_004413"
     CDS             complement(7117..7341)
                     /gene="cspD"
                     /locus_tag="ACHKXP_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO7649283.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7491..7565
                     /gene="yljB"
                     /locus_tag="ACHKXP_004414"
     CDS             7491..7565
                     /gene="yljB"
                     /locus_tag="ACHKXP_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO7649284.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7664..7984
                     /gene="clpS"
                     /locus_tag="ACHKXP_004415"
     CDS             7664..7984
                     /gene="clpS"
                     /locus_tag="ACHKXP_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO7649285.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8015..10291
                     /gene="clpA"
                     /locus_tag="ACHKXP_004416"
     CDS             8015..10291
                     /gene="clpA"
                     /locus_tag="ACHKXP_004416"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO7649286.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         2671 a         2524 c         2918 g         2551 t
ORIGIN      
        1 aatatgtggc ttagctcatg aaaggagcgc aacaagatga tgaatcaaca tataacaaca
       61 tcttaaaaaa aggcctggca ttacgccagg ccttctgcgt taattaatca cgcttttcca
      121 gcagggtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      181 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      241 ggttaacaga agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac
      301 caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcgccg tggatcacca
      361 acaggaaacc tgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      421 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      481 ttttaccact ggcaattaaa tacagcagcg ctgccgcaac aataccgccg acaacctggg
      541 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      601 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcaaaggcc atcgtcagaa
      661 cggtcagacc gaacgccaac gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      721 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
      781 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa aaatatggtc
      841 gttttatagg gccgtcgtaa aaagtgacga cggaaataat gcgcggctat tttaaaaacg
      901 aaggcgagtc attcaccaga taaataaatc cagtaaattt gatttagggc aacagcgggt
      961 taccccatat agtcgtttgt ctgattgaca gtgtagtgca agcaaaagat ttaatccttt
     1021 aggcgtaata aaaaataatt tatcatgcta attatttgat tttgttgttt ttgcagactt
     1081 atcagcaaga gggagtataa cgcgattatt cgctcatttt tcagacattt gccatgctta
     1141 aatgtgatgt catcacgtat tagcaaggcc tttcccgtta tactgccagc gtaaaggata
     1201 agtcacatat ttctggaggg gatatgattc ttgagcgcgt tgaaattgtg ggttttcgcg
     1261 gtatcaaccg tttgtcgttg atgctggaac aaaacaacgt cctgattggg gagaacgcgt
     1321 ggggtaaatc cagcttgctg gacgccttaa ccctgctgct atcgccagaa tcagatctct
     1381 accattttga gcgcgacgat ttctggttcc cgccgggaga tatcaacggg cgagaacatc
     1441 atctgcatat tattttgacc ttccgcgaat cgctgccagg ccgacatcgg gttcgccgtt
     1501 atcggccgct ggaagcgtgc tggacgccat gcaccgatgg ctatcaccgt attttttatc
     1561 gtctggaagg ggagagtgcg gaagacggca gcgtgatgac actgcgcagt tttctcgata
     1621 aagacggaca tccgattgat gtcgaggata ttaacgatca ggcacgccat ctggtgcgtt
     1681 taatgccggt gctgcgcttg cgtgatgccc gttttatgcg ccgtattcgt aacggcacgg
     1741 tgccaaatgt ccctaatgtg gaagtcaccg cgcgccagct cgatttcctc gcccgtgagt
     1801 tatcctcaca tccgcaaaat ctctctgatg ggcagattcg tcagggactt tccgcaatgg
     1861 tacagctgct tgagcattat ttctctgagc agggggccgg acaggcgcga tatcgtttaa
     1921 tgcggcggcg agccagcaat gagcaacgaa gctggcgcta tctggatatc atcaaccgga
     1981 tgattgaccg acctggtggg cgctcgtatc gggttatttt gctcggcctg tttgctactt
     2041 tgttgcaggc aaaaggcaca ttgcgactgg ataaagacgc ccgtccattg ttgctgatcg
     2101 aagatccaga aacccgttta caccccatta tgctttcagt tgcctggcat ctgttgaatc
     2161 ttctgccatt gcagcgcatt gccaccacca actcgggtga gttgctttcg ttaacgccgg
     2221 tagagcatgt ttgccgactg gtacgtgagt cctcgcgcgt tgccgcctgg cgtctggggc
     2281 cgagtggctt gagtaccgaa gatagccgac gcatatcctt tcacattcgt tttaaccgtc
     2341 cgtcatcgct gtttgcacgc tgctggttgc tggtggaagg ggaaacggaa acctgggtta
     2401 tcaatgaact ggcgcgtcag tgcggacatc attttgatgc cgaagggatc aaggtcattg
     2461 agtttgccca gtccgggcta aagccactgg ttaaatttgc ccgccgaatg gggattgaat
     2521 ggcatgtact ggtcgatggc gatgaagcag ggaagaaata tgccgctacg gtacgcagcc
     2581 tgttgaataa cgatcgggaa gccgaacgag aacatttaac ggcgttaccg gcgctggata
     2641 tggaacattt tatgtatcgc cagggatttt ccgatgtgtt ccaccgcatg gcgcaaatcc
     2701 cggaaaatgt accgatgaat ctacgcaaaa ttatctcgaa agcgatccat cgctcttcca
     2761 aacccgatct tgccattgaa gtggcaatgg aggcaggacg tcgtggtgtg gactccgtac
     2821 cgacgctgct gaaaaaaatg ttctcacgcg tgctgtggct ggcgcgcggt cgcgcggatt
     2881 aaccgcgaaa catcgtggcc atttgtggct gaatagcgtc gagcatctca tagcgccgac
     2941 ggtattcagc ccgtttttta ctggcgattt cggcaatctc ttttcgtgct atctgtgctg
     3001 gaaggcggta atggcgttca gcatcacata cgccgccaac cgattcccag aaagcgttgt
     3061 aatcagcgtg gatcttgcct tctttatcgc gataacgcag gctgcggtaa atatgcgttt
     3121 cattgctgac ggcaataatc tgctctacct gcaaacgttg ggcaaacaga caggccgctt
     3181 ccatcacgag gcgtttggga aatagcccgt ggcaggcttt cgtcgcattc tggatttcct
     3241 gatgtggaat ttcccatttt gcgccttgca gtccgccaat aaacatcgtt cttttcccct
     3301 gatattcaca cagggtaaac gtgatctctg ccagaggaat accttcgctg ttgcggaaca
     3361 ggattgtgct gtcaccttct ttatccattg agatcatcat ggtcagctca agcgtgaact
     3421 gctcgccgtt tttgccttcc agcttcgcca gttgcagccc gggggtattc aaatataagc
     3481 tgaattcttc cgccgacata cattcgcgga gtaacgcata atggtaacgt aacgcctcca
     3541 gcaattgctt acggctaaga ttcgccgcaa ggtaagggcg atgcagacgc acaggcagtc
     3601 gcggctggcg cgttaacaat acattgagat taggccagtg ggaaagttcg ttcatccact
     3661 caacgcttaa acgcggcata atcaacgagc gcagcaaaaa tttctggcga aaactacggc
     3721 gatgccagaa tttacccggc cgacactgtc cacgtgccag actaagaaaa agtgacaggc
     3781 tgctgagaga ttcagatggc gtaaaggtcc gttcagttag ctgcgacata ttcatgaaat
     3841 caatggttat acatgacgtc gatttcacca ttgcgtatct taaccaaaca tcaatagtgt
     3901 gattactaac gtaaatttta ggggtttgtt gatatttcgt tgaagttaat gacccggatt
     3961 ggcatatgga gtattcagaa tatttatgaa aaagcggaaa accgtgaaga agcgttacgt
     4021 tattgcgctg gtgatagtca tcgccggact gattacgtta tggagaattc ttaacgcacc
     4081 cgtgccgact tatcagacac tgattgtgcg ccccggtgat ttacagcaaa gcgtgctggc
     4141 gaccggaaag ctggacgcgc tgcgtaaggt tgacgtgggc gcgcaggtca gcggtcagtt
     4201 gaaaacgctg tcggtggcga ttggcgataa agtaaaaaaa gaccagcttt taggggtcat
     4261 tgatcctgaa caggctgaaa accagatcaa ggaggtcgaa gcaacgctga tggagctacg
     4321 cgcgcagcgg cagcaggcgg aagcggagct gaaactggcg cgggtgacgt attcccgtca
     4381 gcaacgtctg gcacaaacgc aggcggtttc acagcaggat ctcgacaccg ccgcgacgga
     4441 gatggctgtg aaacaggcgc aaattggcac cattgacgcg caaatcaagc gcaatcaggc
     4501 ttctctcgat acggctaaaa ccaatctcga ttacactcgc atcgttgccc cgatggccgg
     4561 ggaagtcacg caaatcacca ctctgcaagg ccagacggtg attgccgcac aacaagcacc
     4621 gaacattctg acgctggcag atatgagcac catgctggta aaagcgcagg tttctgaagc
     4681 ggatgtaatc cacctgaagc cggggcaaaa agcctggttt acggtgcttg gcgatccact
     4741 gacgcgctac gaggggcaaa tcaaggatgt actaccgacg ccggaaaagg ttaacgacgc
     4801 tattttctat tacgcccgtt ttgaagtccc caaccccaat ggtttgctgc ggctggatat
     4861 gactgcgcaa gtgcatattc agctcaccga tgtgaaaaat gtgctgacga tccctctgtc
     4921 ggcgttaggc gatccggttg gcgataatcg ttataaagtc aaattgttgc gtaatggtga
     4981 aacacgcgag cgtgaagtga cgattggcgc acgtaacgat accgatgttg agattgtcaa
     5041 agggcttgaa gcgggcgatg aagtggtgat tggtgaggcc aaaccaggag ctgcacaatg
     5101 acgcctttgc tcgaattaaa ggatattcgt cgcagctatc ctgccggtga tgagcaggtt
     5161 gaggtgctga agggcatcag cctcgatatt tatgcgggtg agatggtcgc gattgttggc
     5221 gcttcgggtt ccggtaaatc gaccctgatg aatattctcg gctgtctgga taaggccacc
     5281 agcggcacct atcgcgtcgc cggtcaggat gttgccacgc tggacgccga tgcgctggcg
     5341 caactgcgcc gcgagcattt cggctttatt ttccagcgtt accatttgct ttcgcattta
     5401 accgccgagc agaacgttga agttcccgcc gtctatgctg gtcttgagcg gaaacagcgg
     5461 ctgcttcgcg cccaggagtt gctgcaacgg ctggggctgg aagaccgtac agagtattat
     5521 ccggcacagc tttcgggtgg tcagcaacag cgcgtcagca tcgcgcgggc attgatgaac
     5581 ggcggtcagg taattcttgc cgatgaacca accggcgcac tggacagcca ttctggcgaa
     5641 gaggtgatgg cgatcctgca tcagctgcgc gatcgcgggc atacggtgat tatcgtcacc
     5701 cacgatccgc aggtcgctgc tcaggccgag cgggtgatcg aaattcgcga cggcgaaatt
     5761 gtgcgcaatc ctcccgccat tgaaaaagtg aatgttgctg gcgggacgga gcctgttgtc
     5821 aacacggtgt ctggctggcg gcagtttgtc agcggtttta acgaggcgct gacgatggca
     5881 tggcgggcgc tggcagcgaa taaaatgcgt actttactga ccatgctggg gattattatc
     5941 ggtattgcgt cggtggtttc cattgtcgtg gtgggtgacg ccgccaaaca aatggtgctg
     6001 gcggatattc gttctattgg tacgaatact attgatgtct atcccgggaa agattttggc
     6061 gatgacgatc cgcaatatca acaggcgctg aagtacgacg acttaatcgc catccaaaaa
     6121 caaccgtggg tcgcctcagc cacacctgcc gtctcgcaaa acctgcgcct gcgttataac
     6181 aatgttgatg ttgctgccag tgccaatggc gtgagcggcg attattttaa tgtctatggc
     6241 atgaccttca gtgaagggaa cacctttaat caggagcagc tgaacggtcg tgcgcaggtc
     6301 gtggttctcg acagtaatac tcgccgccag cttttccccc ataaagcaga tgtggttggc
     6361 gaggtgattc tggtcggcaa tatgcccgcc agagtcattg gtgtggcgga agaaaaacag
     6421 tcgatgtttg gtagcagtaa agtgctgcgt gtctggctac cttacagcac gatgtccggg
     6481 cgagttatgg gccagtcgtg gcttaactcc attactgtca gggtgaaaga aggatttgac
     6541 agcgccgagg cggaacagca actcacgcgt ttactttcac tgcgccacgg aaagaaggat
     6601 ttctttacct ggaacatgga cggcgtcttg aaaactgttg aaaagaccac acgtacttta
     6661 caactgtttc tgacgctggt ggcggtgatt tcgctggtgg tgggcggtat tggtgtaatg
     6721 aatattatgc tggtgtcagt gaccgagcgg acgcgggaaa ttggcattcg catggctgta
     6781 ggtgcgcgag caagcgatgt tttgcaacag ttcctgatcg aagccgtact ggtttgcctg
     6841 gtcggtggcg cgttgggaat aacactgtca ctgttaattg ctttcacctt gcagcttttc
     6901 ttacccggct gggagattgg tttttcaccg ttggcgctgc tgctggcgtt tctctgctcg
     6961 acggtcaccg ggattttatt tggctggtta cccgcacgaa atgcggcacg actggatcca
     7021 gtagatgctc tggcacgaga gtaatttttg agataaaaat gccagccgat cgggctggca
     7081 ttttgccttt aggatgtaca caatgagaca gaagagctat gcgactgccg cttctacttc
     7141 gacgggcaca ataacactgg cgtgattgcc ttttggcccc tggtggacat caaactgaac
     7201 ggattgtcca gcttttagcg ttctgtaacc atccatctga atggtggaat aatgagcgaa
     7261 aatatcttcg ccgccgcctt cagggcagat gaaaccaaac cctttggcat tgttgaacca
     7321 cttaacagta cccttttcca tgcttcgaca tccttcgcaa atcttataca agtaagatgg
     7381 aataaaccgg ggtcagagag ggggctgttc aaaacctcgc caactctaga aatacaattt
     7441 agagaattag ggcgagccgt caagcatttg acaggggaca gggggcaggt atgaatcaaa
     7501 aatttgaagc agttaacgct attgacagga atgtgacaga tgtcgctgat gccaacgata
     7561 gatgatagtt atctatcatg tggagtagat tggtcaggca aataagctct tgtcagcggc
     7621 agggcgttct gccgataacc gtaaccgaag atgataactg acaatgggta aaacgaacga
     7681 ctggctggac tttgatcaac tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc
     7741 tatgtataaa gtgatattag tcaatgatga ttacactccg atggagtttg ttattgacgt
     7801 gttacaaaaa ttcttttctt atgatgtaga acgtgcaacg caattgatgc tcgctgttca
     7861 ctaccagggg aaggccattt gcggagtctt taccgccgag gttgcagaaa ccaaagtggc
     7921 gatggtgaac aagtacgcga gggagaatga gcatccattg ctgtgtacgc tagaaaaagc
     7981 ctgaatgcag gcataaaaat tgggggaggt gcctatgctc aatcaagaac tggaactcag
     8041 tttaaatatg gctttcgcca gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca
     8101 cttgttactg gcgctgctca gtaacccatc tgcccgggag gcgctggaag cgtgttctgt
     8161 ggatttggtt gcgctccgtc aggaactgga agcctttatt gaacaaacca cacccgttct
     8221 gcctgccagt gaagaggagc gcgacacaca gccgacgctg agttttcagc gtgtactgca
     8281 acgtgcggtc ttccatgtcc agtcctccgg tcgcaatgag gttaccggtg caaacgttct
     8341 ggtcgctatc tttagcgaac aggagtcgca ggcggcatat ctgttgcgta aacatgaagt
     8401 cagccgtctc gatgtggtga attttatctc tcatggcacg cgtaaagacg agccgacaca
     8461 gtcttctgat cctggcagcc agccaaacag cgaagaacaa gctggtgggg aggaacgtat
     8521 ggagaatttc acgacgaacc tgaatcagct tgcgcgcgtg ggcggaatcg acccactgat
     8581 tggtcgtgag aaggagcttg agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa
     8641 cccgctgctg gtgggggaat ctggtgtcgg taaaaccgcg attgcagaag gtcttgcctg
     8701 gcgaattgtt cagggcgatg tgccggaagt gatggctgac tgtacaattt actctctcga
     8761 tatcggttct ctgttagcgg gcactaaata tcgcggcgac tttgaaaaac gttttaaagc
     8821 gttgctcaag cagctggagc aggacactaa cagcatcctg tttattgatg agatccacac
     8881 cattatcggt gcgggtgcag cgtctggtgg ccaggtcgat gcggctaacc tgatcaaacc
     8941 gttgctctcc agcggtaaaa ttcgcgtaat tggttcgaca acctatcagg agttcagcaa
     9001 cattttcgag aaagaccgtg ctctggcgcg tcgcttccag aaaattgata ttactgaacc
     9061 gtcgatcgaa gaaactgttc aaatcatcaa tggcctgaaa ccgaagtatg aagcgcacca
     9121 cgacgtgcgt tataccgcaa aagcggtgcg tgcggcggta gagctggcgg tgaaatacat
     9181 taacgatcgt catctgccgg ataaagccat tgatgttatc gacgaagcgg gcgctcgcgc
     9241 acgtctgatg ccggtaagca aacgcaagaa aaccgttaat gtggcggata ttgagtccgt
     9301 ggtggcccgt attgcacgca ttccagagaa gagtgtttct cagagtgatc gtgataccct
     9361 gaaaaacctc ggcgatcgct tgaaaatgct ggtcttcggt caggataaag ccattgaggc
     9421 gctgactgaa gccattaaga tggcgcgtgc aggtttaggt cacgaacata aaccggttgg
     9481 ttcgttcctg tttgccggcc ctaccggggt cgggaaaaca gaggtgacgg tacagctttc
     9541 gaaagctttg ggcattgagc ttctgcgctt tgatatgtcc gagtatatgg aacgccatac
     9601 cgtcagccgt cttattggtg cgcctccggg atacgttggt tttgatcagg gcggtttgct
     9661 gactgatgcg gtcatcaagc atccacatgc ggtgctgctg ctggacgaaa tcgagaaagc
     9721 gcacccggac gtgttcaata ttctgttgca ggtgatggat aacggtacgc tgaccgataa
     9781 caacggacgc aaagcagact tccgtaacgt ggtgctggtg atgaccacca acgccggggt
     9841 acgggaaact gagcgcaaat ccattggtct tatccaccag gataacagca ccgatgcgat
     9901 ggaagagatc aagaagatct ttacaccgga attccgtaac cgtctcgaca acattatctg
     9961 gtttgatcat ctgtcaaccg acgtgatcca tcaggtggtg gataaattca tcgtcgagtt
    10021 gcaggttcag ctggatcaga aaggtgtttc tctggaagtg agccaggaag cgcgtaactg
    10081 gctggccgag aaaggttacg accgggcaat gggcgcacgt ccgatggcgc gtgtcatcca
    10141 ggacaacctg aaaaaaccgc tcgccaacga actgctgttt ggttcgctgg tggacggcgg
    10201 tcaggtcacc gtcgcgctgg ataaagagaa aaatgagctg acttacggat tccagagtgc
    10261 acaaaagcac aaggcggaag cagcgcatta atctgattgt caggtaggtt ggtgaagtcc
    10321 gtaatctcga aagaggttgc ggactttttg tttatggggt ggaacggtga aaaccctatt
    10381 tttggaggtg aaggtaagtt gttgataatt agtgctgctg gaaggtaagg ataaaaaagg
    10441 gtgctgcagg agaatgggat ggttttgctt tattaacaac gggctaaacg tgtagtattt
    10501 gagttcactg ccgtacaggc agcttagaaa ttcacaggta acatactcca cccgcccacc
    10561 atgttcactg ccgtacagac agataaaatg cgaaaaaaaa gctcgcactt tcgtacgagc
    10621 tcttctttaa atatggaggt gaggggggat tgactcgctt cgct
//
LOCUS       DAZFZC010000137        10635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFZC010000137 DAZFZC010000000
VERSION     DAZFZC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.138"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..244
                     /locus_tag="ACHKXP_004417"
     CDS             71..244
                     /locus_tag="ACHKXP_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649287.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            270..338
                     /gene="yncP"
                     /locus_tag="ACHKXP_004418"
     CDS             270..338
                     /gene="yncP"
                     /locus_tag="ACHKXP_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO7649288.1"
                     /translation="MNLLVKCAGKIPALALTWTCRP"
     gene            complement(316..465)
                     /gene="hokB"
                     /locus_tag="ACHKXP_004419"
     CDS             complement(316..465)
                     /gene="hokB"
                     /locus_tag="ACHKXP_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO7649289.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            865..2505
                     /gene="trg"
                     /locus_tag="ACHKXP_004420"
     CDS             865..2505
                     /gene="trg"
                     /locus_tag="ACHKXP_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO7649290.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRSLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLAQVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEIDKVTQQNASLVEEASAAALS
                     LEEQAARLTEAVDVFRLHKHSVSAKPRGAGEPVSFATV"
     gene            complement(2543..3466)
                     /gene="ydcI"
                     /locus_tag="ACHKXP_004421"
     CDS             complement(2543..3466)
                     /gene="ydcI"
                     /locus_tag="ACHKXP_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7649291.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGRSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3683..5026
                     /gene="ydcJ"
                     /locus_tag="ACHKXP_004422"
     CDS             3683..5026
                     /gene="ydcJ"
                     /locus_tag="ACHKXP_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7649292.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5251..6906
                     /gene="opgD"
                     /locus_tag="ACHKXP_004423"
                     /gene_synonym="mdoD"
     CDS             5251..6906
                     /gene="opgD"
                     /locus_tag="ACHKXP_004423"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO7649293.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7046..7270
                     /gene="ydcH"
                     /locus_tag="ACHKXP_004424"
     CDS             7046..7270
                     /gene="ydcH"
                     /locus_tag="ACHKXP_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO7649294.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7333..7872
                     /gene="rimL"
                     /locus_tag="ACHKXP_004425"
     CDS             7333..7872
                     /gene="rimL"
                     /locus_tag="ACHKXP_004425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO7649295.1"
                     /translation="MTETIKVSESLELHAVAENHVKPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(7864..8844)
                     /gene="ydcK"
                     /locus_tag="ACHKXP_004426"
                     /pseudo
     CDS             complement(7864..8844)
                     /gene="ydcK"
                     /locus_tag="ACHKXP_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
     gene            8968..9960
                     /gene="tehA"
                     /locus_tag="ACHKXP_004427"
     CDS             8968..9960
                     /gene="tehA"
                     /locus_tag="ACHKXP_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO7649296.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFIARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTLALLMQGKLLVRTERAVLMKAEDKE"
     gene            9957..10550
                     /gene="tehB"
                     /locus_tag="ACHKXP_004428"
     CDS             9957..10550
                     /gene="tehB"
                     /locus_tag="ACHKXP_004428"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO7649297.1"
                     /translation="MIISDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGEYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEKVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         2725 a         2493 c         2710 g         2707 t
ORIGIN      
        1 aacggtatca aaataaacca gcgtataacg ttcaaatcga acataagatt cgataaacca
       61 tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg tttcctttta
      121 tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac actgtacggc
      181 aggcggcaaa tttgtctgta atgatggttc tattagtgca tcgaaaaaaa catgcactaa
      241 ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct agtcaaatgc gcggggaaaa
      301 tccccgcgct tgcccttacc tggacgtgca ggccatgagc gcagcaacct ccttatcacc
      361 gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg agtgtgaatg tcagaatcgt
      421 aatgcagata atgagcagac acaccaccag agggttgtgc ttcatagcct ttctccttgc
      481 cggatggcgg gtaagaggct aagatctgaa ttgctaggtt cattcgttgg cctcggtttg
      541 atagaaatat cggtcggggc cttcgtcttt ctgattcccg gttagcctga aaacagaaag
      601 tctcaggcac ccgcaggcat cctatgaggt ttccttaggg acgaaaataa tcacttcacg
      661 aaattgcgtg ctgttttcca gaatttttcg tcattcgggt tagccagttt agccattcgt
      721 tactctcttc attccaatag cattaatttt ctatgcaata attgttgtaa aaatgtgacg
      781 caaagaggtt tttggtcata agtaattacc gtcaagtgcc gatgactttc tatcaggagt
      841 aaacctggac gagagacaac ggtaatgaat acaactccct cacagcgatt aggttttttg
      901 catcacatca ggttggttcc gttatttgcc tgcattctag gcggtatctt agttctattc
      961 gcattaagtt cagccctggc tggctatttc ctctggcagg ccgatcgcga tcagcgtgat
     1021 gttactgcgg agattgagat tcggaccggg ttagcgaaca gttcagattt tttgcgttca
     1081 gcccggatca atatgattca ggccggggct gcgagtcgta ttgcggaaat ggaagcaatg
     1141 aagcgaaata ttgcgcaagc cgaatcggag attaaacagt cgcagcaagg ttatcgtgct
     1201 tatcagaatc gaccggtgaa aacacctgct gatgaagccc tcgacactga attaaatcaa
     1261 cgctttcagg cttatatcac gggtatgcaa cctatgttga aatatgccaa aaatggcatg
     1321 tttgaagcga ttatcaatca tgaaagtgag cagatccgat cgctggataa tgcttatacc
     1381 gatattttga acaaagccgt taagatacgt agcaccagag ccaaccaact ggcggaactg
     1441 gcccatcagc gcacccgcct gggtgggatg ttcatgattg gcgcgtttgt gcttgcccag
     1501 gtcatgacgc tgataacatt tatggtgcta cgtcggatcg tcattcgtcc actgcaacat
     1561 gccgcacaac ggattgaaaa aatcgccagt ggcgatctga cgatgaatga tgaaccggcg
     1621 ggtcgtaatg aaatcggtcg cttaagtcgt catttacagc aaatgcagca ttcactgggg
     1681 atgacagtag ggactgttcg acagggcgcg gaagagattt atcgtggcac cagcgaaatt
     1741 tcagctggca atgcggacct gtcatctcgc accgaagaac aagcggcggc tatcgaacaa
     1801 actgctgcca gcatggagca actcactgcg acggtgaaac agaatgcgga taacgcgcat
     1861 catgccagca aactggcgca agaggcttct attaaagcca gcgatggcgg gcagacggtt
     1921 tccggtgtag taaaaacgat gggcgctatc tccacgagtt cgaagaaaat ttctgagatc
     1981 accgccgtca tcaacagtat tgctttccag acgaatattc tggcactgaa tgctgccgtt
     2041 gaagccgcgc gagcgggtga gcaagggcgt ggatttgccg ttgtcgccag cgaagtacgg
     2101 acactcgcaa gtcgcagcgc tcaggcggcg aaagagattg aaggcttgat cagtgaatca
     2161 gtcaggttaa ttgacctggg gtcggatgag gtggcaacgg cagggaaaac catgagcact
     2221 attgttgatg ccgtcgcgag tgtcacacat atcatgcagg aaatcgccgc cgcctcggat
     2281 gaacaaagta gaggcataac gcaggttagc caggcgattt ctgaaataga taaggtgacg
     2341 caacagaatg cttctctggt agaagaggcc tcagcggcgg cgttgtccct tgaagaacag
     2401 gcggcacgat taactgaggc ggtggatgta ttccgtctgc acaaacattc tgtgtcggca
     2461 aaacctcgcg gagcgggtga accagttagt ttcgctacgg tgtgaaaatg ttcaaggagg
     2521 gatcgacaga tcccttcacc tttcagaacg gcattgattt tcgaatagcg ttaatcatca
     2581 actggcaacc agaagagaac gtcgcatcta cgcgggtcag tattccaatc ggttcgcctg
     2641 caccatgtcc cggaacaggc agggccacca gcgtggcatg acgcaggtcg tcttttaccg
     2701 cgccagaagg gacaaaccac acgtagtcgt attcaaccgt aagttgacga gatagcgaag
     2761 cagacagcgt ttcgatacaa cccgaaggaa ttttacatcc ctggctctgc actaatgcat
     2821 ctgaatgctg gcgtggcgca gtgccttctg gtgatacaac gaccggccat tccagcaccc
     2881 ggcttagcgt tacgttctcc tgaagtagcg ggtgattagg gcggacaacc agcttcaacg
     2941 attcaagaaa cagcagttcg taattaagcc cggtcatcag ttcaggatct gacatccgac
     3001 caatgccgat atcgatttcc ccggttttta aacccgccag aatcataggg ttactcattg
     3061 tcgcaacttg caaggtcgtc tctttttgtt gctgatgaaa ctgacctata accgaaggta
     3121 atatccccag tgccgcagta ggtagtgcac caaccctgac gacatcatta ttaagacctt
     3181 ctttacgatg aagcgaccgt ccggcagtgt tgatggcgtc aagaactctg actgcatgcg
     3241 ttaaaaattg ttcgccgggt aaggtaagtt gcgccccctg acgaccacgc tcaaacaagc
     3301 gagcgccagt cagctgctcc agttcattca atgtcttaga gagcgcaggt tgactcaaat
     3361 taagggtttc agccgcgcgc cccaaagttc cttgttgtgc gacagctacg aatgtatgaa
     3421 ggtggcgcaa acggatgcgc tgactaaaca gactattttt ttccataagc gatgttaaaa
     3481 acgaagcggt gtcgctgaca agtgaagttg tttgattatg ataacttgat tgcaaaatat
     3541 tattaacaat taaagcaatt atgttacagc aaaatggata atattgatgt tttcgcggcg
     3601 agatcacagt ttgtaaattc ttcccgcaag agtgaatgcg gttacctaca ctccagatta
     3661 ctgaccactg gaggcagaca ctatggcgaa cagcatcacg gcggatgaga ttcgggaaca
     3721 gttttcgcag gcaatgtcag ccatgtacca gcaagaagtt ccgcaatatg gcacgctgct
     3781 ggaactggta gctgatgtga atctggctgt gctggaaaac aatcctcaac tgcacgaaaa
     3841 aatggtaaat gcagacgagc tggcgcgact gaatgttgaa cgtcatgggg cgattcgcgt
     3901 tgggactgca caagagcttg ctactcttcg gcggatgttt gccattatgg ggatgtaccc
     3961 ggtgagctat tacgatctct cgcaggcagg ggtgccggta cattcgacag catttcggcc
     4021 cattgatgat gcttctctgg cgcgtaatcc cttccgcgtt tttacctcct tactccgcct
     4081 tgagcttatc gagaacgaaa ttttgcgcca gaaagcggcg gagattctgc gtcagcgcga
     4141 tatcttcacc ccacgttgtc gacaactgtt agaggaatat gagcagcagg gcggttttaa
     4201 cgaaacacag gcacaggagt ttgtgcagga agccctggaa acgtttcgct ggcaccagtc
     4261 agcaacggta gatgaagaaa cctatcgcgc attgcacaac gaacatcggt tgattgctga
     4321 tgtggtctgt tttcctggat gccatatcaa ccacctgacg ccacgtacgc tggatattga
     4381 ccgggtgcag tcgatgatgc ctgaatgcgg aattgaaccc aaaattctca tcgaggggcc
     4441 gccgcgccgc gaggtaccga ttttactacg ccagaccagc tttaaagcac tggaagagac
     4501 ggtgttgttt gcggggcaga aacagggcac gcataccgcg cgctttggtg aaattgagca
     4561 gcgtggcgtg gcattaacgc cgaaagggcg acaactgtat gatgatcttc tgcgtaacgc
     4621 tggaaccggg caggataatc tcactcacca aatgcattta caggaaacct tccgcacttt
     4681 tcctgacagt gagtttttaa tgcgtcagca agggttggca tggttccggt accgtctgac
     4741 gccttcgggt gaggcgcatc gtcaggcgat tcatcctgga gacgatccac agcccttaat
     4801 tgaacgtggt tgggtagtgg cgcaacccat cacctatgaa gatttcttgc ccgttagcgc
     4861 ggcggggatc ttccagtcaa atctgggtaa tgaaacgcag acacgcagtc acggtaatgc
     4921 cagtcgcgaa gcatttgagc aggcgttggg ttgtccggtt ttggatgagt tccagcttta
     4981 ccaggaagcg gaagaacgca gtaaacgtcg ctgtggtttg ctttaaaatc tgaccatccg
     5041 cctttgcaaa aatttgcctg attttacaaa cgaatcaggc tcatcccatc gacataaaaa
     5101 aaatgccgat ttatgcatat tctctcagtt caacaattgg attattaata aatattgtct
     5161 agagtgagcg gtcataaata agcactttct tgccgctgaa aacgaccagc gcgggaccat
     5221 tcacaacacc agaaggactc actttcaggt atggatcgta gacgatttat taaaggttca
     5281 atggctatgg ccgccgtgtg cggtaccagc ggcattgctt ctcttttttc tcaggcggca
     5341 ttcgcggcag attctgatat tgccgacggg caaacccagc gttttgactt ctccattcta
     5401 cagtcaatgg cgcacgactt agcgcaaaca gcgtggcgtg gtgcgcctcg tccgttacct
     5461 gacacgctgg cgacaatgac gccgcaggct tataacagta ttcaatacga cgccgaaaaa
     5521 tcgctctggc ataacgttga gaaccgtcaa ctggacgctc agttcttcca tatgggaatg
     5581 ggattccgtc gccgcgttcg tatgttttct gtagatccag caacacatct ggcgcgtgaa
     5641 attcactttc gcccggagtt gttcaaatac aacgatgcag gtgttgatac aaaacaatta
     5701 gaagggcaaa gcgatctcgg ctttgccggt tttcgcgtgt ttaaagcccc cgaactggcg
     5761 cgccgtgatg tagtatcatt tctcggcgcg agttatttcc gcgccgttga tgatacatat
     5821 caatacggtt tgtcggcccg cggcctggcg atcgacactt acaccgacag taaagaagag
     5881 ttccccgact ttaccgcctt ctggtttgat acggtaaaac cgggggcaac tacctttacc
     5941 gtttatgcgt tgctcgatag cgccagcatt actggtgcct ataagttcac tatccattgt
     6001 gagaaaagtc aggtgattat ggatgtggaa aatcacctgt atgcgcgcaa agacattaaa
     6061 cagctgggca ttgcgccgat gaccagtatg ttcagctgcg gtactaatga acgtcggatg
     6121 tgcgatacaa ttcatccgca aattcatgac tctgatcgtc tgtccatgtg gcggggcaac
     6181 ggcgagtgga tttgccgtcc gctgaataat ccgcaaaaat tgcagttcaa tgcttacacc
     6241 gacaacaacc cgaaagggtt tggtttattg caactggatc gtgacttctc ccattatcag
     6301 gacattatgg gctggtataa caaacgccca agtctgtggg tggaaccgcg taacaagtgg
     6361 ggtaagggca ccatcggcct gatggaaatc ccaacaacgg gcgaaacgct ggataacatt
     6421 gtctgcttct ggcagccaga aaaagctgta aaagcaggtg atgagtttgc attccagtat
     6481 cgtctgtact ggagtgcgca accgcctgtt cattgcccat tagcgcgcgt tatggcgacg
     6541 cgtaccggca tgggcggttt cccggaaggt tgggcgccag gtgaacacta tcccgaaaaa
     6601 tgggcgcgtc gttttgccgt cgatttcgtt ggtggtgatc tgaaagctgc cgcgccaaaa
     6661 ggcattgagc cggtgattac gctttccagt ggggaagcga agcaaatcga aattctctat
     6721 attgaaccca tcgatggtta tcgtattcag tttgactggt atccgacttc ggactccact
     6781 gatccggtcg atatgcggat gtatctacgt tgtcaggggg acgctatcag tgaaacatgg
     6841 ctgtatcagt atttcccgcc agcgccggat aaacgtcagt atgttgacga ccgcgtgatg
     6901 agttaatcgt tttttcttcg gcaccttctt cgggaggtgc cgtctggtta aacacgatcc
     6961 cgctcgcatt tttccctaag ttaaatgagt aatctgatgg tgtgtatttc agatacacct
     7021 tgtcagccac taacagggag tgcgtatgtt tccagaatac cgagatttaa tatcccgtct
     7081 gaaaaacgaa aatcctcgct ttatgtcctt gttcgataaa cacaataaac ttgatcatga
     7141 aattgccaga aaggaaggtt ccgacggtcg agggtacaat gcggaagtgg tccgcatgaa
     7201 aaaacaaaag ctacagttaa aagatgagat gctcaaaatc ctgcagcagg agagcgtcaa
     7261 agaggtgtaa actttcctaa gccgccagcc aggcggcttt tttaacaact gcatggattg
     7321 actggagata agatgactga aacgataaaa gtaagcgaat cacttgaatt acatgctgtt
     7381 gcagaaaatc acgtcaaacc tctttatcag ttaatctgta aaaataaaac ctggttacag
     7441 cagtcgctaa actggccgca gtttgttcaa agtgaagagg acacgcgaaa aacggtgcag
     7501 ggtaatgtga tgttgcatca acgcggctat gccaaaatgt tcatgatttt caaagaagat
     7561 gaacttatcg gcgttatctc gtttaatcgt attgaaccac tgaataaaac cgctgaaata
     7621 ggctactggc tggacgaatc tcatcagggg caggggatca tttctcaggc gctgcaggca
     7681 ttgattcatc attacgccca gtctggtgaa cttagacgct tcgtgatcaa atgtcgggtg
     7741 gacaatccgc aaagcaacca ggtcgctttg cgcaatggtt ttatccttga aggttgcctg
     7801 aaacaggctg agttcctgaa tgatgcctat gatgatgtga acttatacgc gcgtattatc
     7861 gattcacaat aaccctgcca gcggcgttcg cgtaatgcgc tcctcgccgt taatgacttt
     7921 tggaccacgg acatgaaccg tgtcgccatc aaaggcttca acgacggcgt ggtcggttaa
     7981 ctccacatgg ttttcaatta tcaccgcacc tgtaatacgg ctctcacctt gtatgacaac
     8041 gtgctcgtcg agcagaatcg gtccgccacg taccacggca tttccgccaa ccaagacatg
     8101 atgttttaac acacaattac cttccacaat ggcatattcc gccacctgcg aactgtaatg
     8161 aatcgtcgga atggcatctt cttctatgcc agctttcacc tgcgcgtgac cgtagacttt
     8221 agcgcaatcg catagccaga cattgttctc ttaattacct tcaatactgg caaaatcaaa
     8281 aacttcggcg cgatgttcaa taaaagcata ccggatgacg gcatcgccat aaatttgtgc
     8341 ctggtggaca atacgcgagg cgctaaccct tgcgcgatca taaatttgta gcaggagttg
     8401 atggtcaggc gttaagcctt gtgctgcgac gatcatagaa tgttgattaa ttaaggcgtg
     8461 accaaatatt ctacattgcc cgcaaaccag tgagtcatga atggtgacgc tgtcacttat
     8521 atatgcaccc tggctgattt cactattatc gatccagaca ttatccgttg cgtaaacctc
     8581 tccccacagt acgctggtgc cggtgatgcg cgtattaccg gaaatcaccg ttccgccgaa
     8641 cgcaatagca ttctggtcgt aaatccagca atttccttcc tgtgctaaca ccgtttcgcg
     8701 atcgatccag ccgccagcgg ttcctgctat cacatcgtta aaatcgctaa tggcgataat
     8761 ctgccgtaat aacacacttt ttttagtgtc atcttcctga taactaaagg ctcgctgttc
     8821 ttcacttaga cgatatttgc gcatagcatt ttcccacagg tgacttacta taaccgtagc
     8881 aaattctgcg gctctggcta tgctcgagaa aattccataa aatgcatttc aaatatactt
     8941 tataaattaa acaaaatgag taagaagatg cagagcgata aagtgctcaa tttgccggca
     9001 ggctactttg gtattgtgtt ggggacgata gggatgggat ttgcctggcg ctatgccagc
     9061 caggtttggc aggtcagcca ctggttaggg gatgggctgg tgattctggc gatgatcatc
     9121 tggggattgt taactagcgc atttattgcc cgactcatac gctttccgca tagcgtgctg
     9181 gcggaagttc gccatccagt gctgagcagt tttgtgagtt tgtttccggc aacgacgatg
     9241 ctggtggcga ttggttttgt tccgtggttt cgcccactgg cggtgtgcct gttcagtttt
     9301 ggtgtcgtgg ttcagttggc ttatgccgcc tggcaaactg cgggattatg gcgcggatct
     9361 caccctgaag aagctaccac gcctggactg tatctgccga cagttgccaa caactttatc
     9421 agcgcaatgg cctgtggtgc gttgggctac accgacgccg gtctggtgtt tttaggcgca
     9481 ggcgttttct catggctaag ccttgaacct gtgatcttgc agcgtctgcg cagttcggga
     9541 gaattaccca cggcactgcg gacatcactc ggcattcagc tcgctcctgc gctggtggcc
     9601 tgtagtgcct ggcttagcgt caacggcggc gagggtgaca cgctggcgaa aatgcttttc
     9661 ggttatggac tgctgcaact gctgtttatg ctacgtctga tgccatggta tctctcccag
     9721 ccatttaatg cttcattctg gagtttctcg ttcggcgtat ctgcactggc aaccaccggt
     9781 ttgcatctgg ggagtggcag cgataatgga tttttccata cgctggcggt gccgctgttt
     9841 atctttacca attttattat tgcaatactg ctcatccgta ctttggcgct tctgatgcag
     9901 ggaaaattgt tagtcagaac cgagcgcgcc gttttaatga aagcagagga caaagaatga
     9961 tcattagtga cgaaaattat tttactgata aatatgaatt aacccgcaca cactctgaag
    10021 tactggaagc ggtgaaagtg gttaaaccgg gtaaaacgct ggatctgggc tgtggcaatg
    10081 gtcgtaacag tctttacctg gcagccaatg gttatgatgt tgacgcatgg gataaaaatg
    10141 ccatgagtat cgccaacgtc gagcgcatta aatccattga aaatctggat aatttacaca
    10201 cccgggtcgt tgatctgaat aacctcacat ttgatggaga gtacgatttt attctttcga
    10261 ctgtggtgct gatgttcctt gaggctaaaa ccatccccgg gttgattgcc aatatgcaac
    10321 gttgcacgaa acctggcggt tacaacctga ttgtggcggc gatggatacc gctgattatc
    10381 catgtaccgt cggcttcccg tttgccttca aagagggaga attacgtcga tattacgaag
    10441 gctgggagaa ggtgaaatac aatgaagacg tcggcgagct gcaccgcacc gacgccaacg
    10501 gtaatcgtat taaactacgt ttcgccacga tgctggcacg taaaaaatga cccggtaaga
    10561 acaaagcgcg tgaaaattcc ccacgctgag atagtttact gttcttcttt tcggtaagca
    10621 tattttttat cgaag
//
LOCUS       DAZFZC010000138        10586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFZC010000138 DAZFZC010000000
VERSION     DAZFZC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.139"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..2419
                     /gene="ycgH"
                     /locus_tag="ACHKXP_004429"
                     /pseudo
     CDS             1..2419
                     /gene="ycgH"
                     /locus_tag="ACHKXP_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YcgH"
     gene            complement(2476..2811)
                     /gene="ymgD"
                     /locus_tag="ACHKXP_004430"
     CDS             complement(2476..2811)
                     /gene="ymgD"
                     /locus_tag="ACHKXP_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO7649298.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(2815..3159)
                     /gene="ymgG"
                     /locus_tag="ACHKXP_004431"
     CDS             complement(2815..3159)
                     /gene="ymgG"
                     /locus_tag="ACHKXP_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO7649299.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3161..3334)
                     /gene="ymgI"
                     /locus_tag="ACHKXP_004432"
     CDS             complement(3161..3334)
                     /gene="ymgI"
                     /locus_tag="ACHKXP_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO7649300.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3435..3620
                     /gene="ymgJ"
                     /locus_tag="ACHKXP_004433"
     CDS             3435..3620
                     /gene="ymgJ"
                     /locus_tag="ACHKXP_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO7649301.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYQKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3581..3700
                     /locus_tag="ACHKXP_004434"
     CDS             3581..3700
                     /locus_tag="ACHKXP_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7649302.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            3735..4078
                     /locus_tag="ACHKXP_004435"
                     /pseudo
     CDS             3735..4078
                     /locus_tag="ACHKXP_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4450..4716)
                     /gene="minE"
                     /locus_tag="ACHKXP_004436"
     CDS             complement(4450..4716)
                     /gene="minE"
                     /locus_tag="ACHKXP_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO7649303.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(4720..5532)
                     /gene="minD"
                     /locus_tag="ACHKXP_004437"
     CDS             complement(4720..5532)
                     /gene="minD"
                     /locus_tag="ACHKXP_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO7649304.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5556..6251)
                     /gene="minC"
                     /locus_tag="ACHKXP_004438"
     CDS             complement(5556..6251)
                     /gene="minC"
                     /locus_tag="ACHKXP_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO7649305.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPTPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            6771..7139
                     /gene="ycgJ"
                     /locus_tag="ACHKXP_004439"
     CDS             6771..7139
                     /gene="ycgJ"
                     /locus_tag="ACHKXP_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO7649306.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(7242..7643)
                     /gene="pliG"
                     /locus_tag="ACHKXP_004440"
     CDS             complement(7242..7643)
                     /gene="pliG"
                     /locus_tag="ACHKXP_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO7649307.1"
                     /translation="MKIKSIRKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            7885..8178
                     /locus_tag="ACHKXP_004441"
     CDS             7885..8178
                     /locus_tag="ACHKXP_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO7649308.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            8250..8909
                     /gene="ycgM"
                     /locus_tag="ACHKXP_004442"
     CDS             8250..8909
                     /gene="ycgM"
                     /locus_tag="ACHKXP_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7649309.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            9001..9447
                     /gene="ycgN"
                     /locus_tag="ACHKXP_004443"
     CDS             9001..9447
                     /gene="ycgN"
                     /locus_tag="ACHKXP_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO7649310.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(9654..10553)
                     /gene="hlyE"
                     /locus_tag="ACHKXP_004444"
     CDS             complement(9654..10553)
                     /gene="hlyE"
                     /locus_tag="ACHKXP_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO7649311.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWFGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKNSYFQSQVDKIRKEAYAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFF
                     TTLSNTVKQANKDIDAAKLKLTTEIVAIGEIKTETETTRFYVDYDDLMLSLLKEAAKK
                     MINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         2845 a         2285 c         2483 g         2973 t
ORIGIN      
        1 ataatgccag tgtgatgact agtggtgatg gatcttatgg tatttctgtt gatagtcaaa
       61 acaaagtatt atatataagc gatagcgata ttaagacctc tggaagcgta tctgacaaag
      121 aaaatggagg gataacagcc agcgcagtag ttagtgaatt tggtggcacc atctttatga
      181 atggtgataa ttcagtcgag tcgggtgggg catattcagc gggactttta agccaggtta
      241 atgattctga aaagatggta aataacaccc gtcttgaaac cacagataaa acgaacattg
      301 ttacctctgg ggaaaatgca gtaggtgttc ttgcatgttc aagtcctgga gagtctcgaa
      361 catgtgtcga tgctgtagat gatgaagtta gtgattctaa cagttacgaa gttattagcc
      421 gtgctgattt aaaaatgaat ggtggttcca taacaactaa tggcattaat agctatggtg
      481 cttatgctaa tgggaaaaaa gcatatatta atttagatta tgtggcactt gaaactgtgg
      541 ctgatggaag ttatgcagtt gctattcgac aaggtaacat tgatataaaa aatagttcta
      601 ttacaacaac aggcactaaa gcccccattg caaaaatata caatggtgga gagttatttt
      661 tttccaatgt caccgcggta tcaaaacaag ataaaggaat atcaattgat gcatcaaata
      721 tcgattctca agccaaaata gcactattaa gtgttgaact ttcaagtgct ttggatagta
      781 ttgatgttaa caaaactaca acggatgtaa gtatccttaa tcgaagtatt atcacacctg
      841 gtaataatgt tctggttaat aatactggag gtgacttaaa cataatttcg tccgactcta
      901 ttctaaatgg agcgactaaa ctcatcagcg gcacaaccac gctgaagctt tcagaaaata
      961 caatctggaa tatgaaagat gactccgttg ttacccatct gactaattca gacagtatta
     1021 tcaatctttc gtatgatgat ggtcaaacat ttacccaagg aaaaacatta accgtaaaag
     1081 gtaattatgt cggtaataat ggtcagctta atatccgcac cgtattaggt gatgataaat
     1141 cggctacgga cagacttatt gttgagggta atacttcggg ttcaactacc gtctatgtga
     1201 aaaatgctgg aggaagcggc gcggccacgc taaacgggat cgaactcata actgtgaatg
     1261 gcgatgaatc tccagcagat gccttcagat aaggtgatgc aaggattgca gctggagctt
     1321 tcgaatatca actaaaacaa caaggcaaaa actggtattt gacaagttat cagtcagtga
     1381 atgaggagga taacagctca gagggaaatt cagaatcaac agaaacgcct acccccgtcc
     1441 tgcgccccga agctggaagt tacgtcgcta acctggcagc tgctaacact ctttttgtta
     1501 tgcgtctgaa cgaccgtgcg ggtgaaacgc gctacatcga tcctgtaact gaacaggagc
     1561 gttcaagccg actttggcta cgtcaaattg gcgggcataa tgcctggcgt gacagcaacg
     1621 gacagttgag aacgacctcg catcgctacg tctcgcagtt agggggcgat ctgttaaccg
     1681 gtggttttac cgatagtgac agttggcgtt tgggagtgat ggctggttat gcccgcgact
     1741 acaacttaac tcattccagc gtgtcggatt atcgttcgaa agggagtgtc agaggctata
     1801 gcgcagggct gtatgccacc tggtttgccg atgacatcag taaaaaaggc gcatacattg
     1861 actcctgggc gcaatatagc tggtttaaaa actcggtgaa aggggatgaa ttagcctatg
     1921 aatcctatag cgcgaaaggt gcaaccgtct cgctggaagc gggttacggt tttgccctga
     1981 ataaatcctt tggtctggaa gcggcgaaat atacgtggat cttccagcca caggcacagg
     2041 ctatctggat gggcgtcgat cataatgcgc acacggaagc caatggctca cgtattgaga
     2101 atgacgcaaa taacaacatc cagacccgac tcggcttccg cacctttatt cgtactcagg
     2161 agaaaaacag cggtccgcac ggtgacgact ttgaaccttt tgttgaaatg aactggatcc
     2221 ataacagtaa agattttgct gtctcaatga acggtgtgaa agtcgaacaa gatggggcga
     2281 gtaatttggg ggaaattaaa cttggcgtaa atggcaacct gaatccagcg gccagcgtct
     2341 ggggaatgtg ggcgtgcagc tgggtgataa tggctacaat gacaccgcag tgatggtggg
     2401 cctgaaatat aagttctgat cccgccgtta gctaaaaaac cgcgtcgtat tcatcgacgc
     2461 ggtacatatg aaatattatt ttttgccgat agcacgcatg gtgtcatcaa ttgccgtgat
     2521 caacagcatt tgcgggtctt tagcgcaaac ctgattcagt ttttctacca ctttggcgct
     2581 cagttccgga gattgctcaa tttttaaatc acggctggca acgctggcat tacccattac
     2641 cgcaacaatt tctgcaacct gtgcgctgtc agtttttgcc atttcgttgg cttctgcgca
     2701 agtaatatag gtttctgacg gcaaaccgtt tttaatattg tagtcctgcg cccaggtcat
     2761 tggtgcgaaa acaaacaggc ccgccagtaa agcaaatttt ttcatcatca ttccttattt
     2821 cattttaccc agaattgcac cacccgtacc gccaatcacg gcacctttaa tcgccccttc
     2881 gaggccattg ccggtcagaa cgccagtgac agcaccaacg gcggcaccca cttttgcacc
     2941 tttacgcgca tttttaccgt cgcggccttt ttctgttact gcaccaacac cagcgccaac
     3001 agctgcacct ttcagtacgc cattaacacc attgccagta agtaaaccaa cgcctgcgcc
     3061 tagcaatgca cctttcgtgg tgcggttcat atccgccatc gctggcgtgg agcagaacaa
     3121 tgctgagata agcccgaagg caagtatttt tttcttcaac ttagatgtcc ggtattaagt
     3181 aagttgcaca cacaataatt tcgtcttcaa ttaagatctg cttaactaaa gaacgctcgc
     3241 tattattcag ataattcaaa atgagcgtgg ctgtgatgat aggaattatg ttttttacgt
     3301 gaatgagaat aatcttaaat gaggaataac tcattgattg acaatatttt tattcaagaa
     3361 gtgtcattga ctgttaacgc aatgttgtaa aggtaagata atctgattta tcaatattat
     3421 tgtgtgattt ttatgtgagc agaagatatt catcagcaac gattacatta gtcattttat
     3481 tttgccgacg gcctcattgt cgaaagataa gcgtacgaca gtattatcag aaaagagtga
     3541 ttttttatcc aactacactt cagcgcactg cgtgtaaaaa atgcctcttt cttatgcggg
     3601 atatcatcat ttcatcatga tgtctttggt gagcggtgaa cacaatacac ctgcgctgtc
     3661 tcttcaggat gaatcccctg gtctggtgcc tgcgggctga tgttgcagca gagcttaggt
     3721 cacttagacg ctactatcat ttatccaatg gcatggaatc gaaatcagtc gatacccgca
     3781 gtatatatcg tgaactgggt gcaacgctga gttacaacat gcgcctgggg aacggtatgg
     3841 aaatgaaccc tggctgaagg cggctgtgcg caaagaattt gtcgatgata accgggtgaa
     3901 ggtgaataat gacggtaatt tcgtcaatga tttgtcgggc agacgtggaa tataccaggc
     3961 agctattaaa gcctcattca gcagtacgtt tagcggacat ctcggggtgg ggtatagcca
     4021 tggtgccggt gtggaatccc cgtggaacgc ggtggctggt gtgaactggt cgttctgacc
     4081 atcaacgatt aaactgcgct tcggcgcagt tttcgtttac aggatgttga aagggaaaat
     4141 tctggggcaa aaaaagcccg ccagttacgg cgggaaacct catcctatgg gagaacaatg
     4201 aataatgaaa ttgcggggtt atcatctccc agtatatcca tactaacaat aaggttattt
     4261 actcaaccag gcataaacat tttgttttgt gcgtgggaac agccttaagg tgtaaagggg
     4321 gaggtggaaa tagcaatgag gagtatcagc aagaatactc gccgctttac cacaacgtgg
     4381 atgagaggga tgaaaaactc aaggcagaga taactctgcc ttgaagataa atgcgctttt
     4441 acagcgggct tatttcagct cttctgcttc cggtaaggtc acgttcagct caagaataga
     4501 aatatcgcca tctttttgct caagctgtac ggttaccatc tcaggatcaa tttgtacgta
     4561 tttacaaatg acctcaagaa tatctttacg caactgcggc agataatgcg gttctgcatc
     4621 gctgcgacgg cgttcagcaa caataatctg cagccgttct tttgcaatgt tggctgtgtt
     4681 tttcttccgc gagagaaaga aatcgagtaa tgccataact tatcctccga acaagcgttt
     4741 gaggaagcct ttcttctctt cttcaatgaa gcggaaagga cgttcttctc ccaacagacg
     4801 ttctacggta tctgcgtagg ctttacccgc atcggcgtta atgtcgagaa tgaccggttc
     4861 accctggtta gaggcgcgca atactgattg atcctctggg atcacgccga cgagtttgat
     4921 gcgcaggatc tccagcacat cttccatgct cagcatgtca cctctgctta cgcggcctgg
     4981 gttatagcgc gttaacagca ggtgctcttt aataggctct tcgccatttt ctgcgcggcg
     5041 tgatttcgac gccagaatgc ctaaaatacg gtcagagtcg cgtactgagg agacttccgg
     5101 gttggtggta ataatggctt cgtctgcaaa atagagtgcc attaacgcac cggtttcaat
     5161 ccctgccggg gagtcacaaa cgataaattc aaaatccatc gctttcagat catcaagaac
     5221 tttggcgacc ccttcacggg tgagggcatc tttatcgcgt gtttgcgatg ccggcagaat
     5281 atagagattt tcagtacgct tatctttaat taacgcctga tttagcgttg catcgccctg
     5341 aatgacgttg acgaaatcgt aaacgacccg gcgttcacaa cccataatca ggtcgagatt
     5401 acgcaggccg atatcaaaat ctatcacgac agttttcttt cccttctggg ccaaaccagt
     5461 ggcgatggcc gcgctggagg ttgtcttacc aacaccccct ttgcccgaag taacaacaat
     5521 aatgcgtgcc atagaaattc cttgttaaaa agggatcaat ttaacggttg aacggtcaaa
     5581 gcgttttcga ctaactgcag tcgcgccgct ttgccataaa attctgctgg gatttgatca
     5641 ctcagccagt attcacctgc gatggacacc agttccgcca tcaggttcgt acaaaatatt
     5701 tgcgtttccc ggtcaccact tgcccctgcc agcgcacgac cgcgcatcat gccatagaca
     5761 tgaatgttcc catcggcaat caattcggcc ccagcgctaa cgtggcttgt aacaatcaga
     5821 tcacattgtg gagcataaat acgctgaccg gaacgcaccg gggtatctat taaacgcgtt
     5881 tttgtgaccg gcgttgtatt ttgcgctgga gcctgcggtg tgggagctgg acgtggcgct
     5941 ttttcctttc cttccgtcag gataggcagc cccatctttt caatttcggc tttaagttgc
     6001 gcatctttgc agccacttac gccaataacc cgcaaaccgg ttgccgaaac cgccttatgc
     6061 atcgctgacc agtttaccgg gtcttccagt gcactgacgt tgagtacaac gggggcatgt
     6121 tttaaaaatg cgggggcctg agcgattttg tcttccagcg cctgatggat aaccttaggt
     6181 tctgcctcat gcagatgaac cacagataaa gtgaagctac tgcctttaag ctcgattggc
     6241 gtgtttgaca tcctggcctt actcaattag ctattaatca tcgccagcgc gcgatgatgt
     6301 tccgaagact ataaggcatg ttatagtctg gattatattg aggcaagtca ccctcccatt
     6361 tattcagagt aaaagtctat tctgtgataa atggcgctga ttcatagctt aaaaaatacc
     6421 cttgtcaatc aacccattgc cgtcgtactt ttgattgttc ttatttacgc ttcctttttc
     6481 cgcaccctaa tcgtgacagc atctccagac ctgtttaaat atccagtatt gaggcaagtg
     6541 tattttttca ccacgccagg ctttcttaac tttccatttt ttgcggcatc attaaagaac
     6601 agatacccgt tatcttaata tccgccacag aattgctcta tcatagaaac cgcagaagac
     6661 gtctgatact tctgtcaggt catcgtaact cgtccaggtc agcggttttg attacggatt
     6721 gatgatgaaa tgagcataag tgttgtacgg ttggattatc aggatgtgct atgaatatga
     6781 tgcgtatttt ttatatcgga ttgtcaggtg tggggatgat gttctcatca atggcgtctg
     6841 gaaatgacgc tggcggactt caatctccgg cgtgcggcgt tgtttgcgac ccgtatattt
     6901 gtgtgaactc agatggcatt tccccagagt taacaaggaa atatctcggc gaaaaagccg
     6961 ctgaaaactt acaatcatta caaggctacg atcccagcga atttacattc gctaacggtg
     7021 tattttgcga tgttaaagaa aaattatgtc gtgatgatcg ttattttggt gtggatggta
     7081 agcggagtgg aaaaatcaat caaaccacca caaagatgtt atttatgtgt cgtgagtgat
     7141 gtcttgttta cattatgttc tattcgtcat taacctcttg agcgacagag aggagtgggg
     7201 cattaacgtt tatgacgggg agagtccccg tcggttgaca tttacttaat ttgaatatcg
     7261 acgttatatt ttttagtttt gtttttacgg gcgtcgtttc gggtttgcaa cactcgcagc
     7321 tcatatttgc cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat
     7381 ctggataaat caactgaatc atcgattcct ggcccgaaga ggtaagtatc agcgccttca
     7441 ttagagatac tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa
     7501 tcgtatccct ttatttcgcc ggagtactga gcgctgctat ggccttttct gaactcaaca
     7561 ttgacattct tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc
     7621 ttcctgatgc tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt
     7681 tataatataa caatccctaa cgggagaatt ccagtggctt tcctacgttc ggtaaaatca
     7741 cgttgcggca tttttgccgg ggggattact tataaagggg gcatggactc aacaaaccgt
     7801 agaaatctac agctggctgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa
     7861 cctggcatct taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac
     7921 cagacctatt tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg
     7981 aaaggttttg gtcagcctca gttagcgatg attctgccgc tggatgggcg gaagaaactg
     8041 gtcaatgccg atattgaaaa ggttaaacag gcattaaccg agcaaggtta ctatttgcag
     8101 ttaccgccac cacccgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca
     8161 gacgacacta acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt
     8221 catatccacg ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc
     8281 tgctggatta tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta
     8341 aagagatggg cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac
     8401 tgtgcgatct gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag
     8461 tcgaactggc ggtgttgatt ggcgcgacgc tgcgtcaggc tacggaagag catgtccgca
     8521 aagccattgc cggttatggc gtggcgctcg atctgacgtt gcgtgatgtt cagggaaaaa
     8581 tgaagaaagc cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt
     8641 ccgggtttat tcccgcggcg gaatttaccg gcgacccgca aaatacaacg ctgggcctga
     8701 gcgtaaacgg cgagcaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc
     8761 cgctgatcgc ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag
     8821 gcacgcctga tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg
     8881 ggcattcttt gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa
     8941 cttgcatcgc tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg
     9001 atgagcgatg tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg
     9061 gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc
     9121 gacgaaatct acttcactaa cgtcgcctgt cgccagctca atattaaaac ctgtcaatgt
     9181 cggaactacg aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat
     9241 ctgccaacat tcgaatggct gccaatgacc tgcgcttatc ggttgctggc ggaaggtaaa
     9301 gatttacctg cgtggcatcc gctacttacg ggttcgaaag cggcaatgca tggtgaacgt
     9361 atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc
     9421 ttaaataaac ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta
     9481 atatacgtct ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg
     9541 agagttaccc gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt
     9601 ttttaaatgt gataaacctt ataccttgag tacatggaga gaataatagc ttatcagact
     9661 tcaggtacct caaagagtgt ctttttaccg tgtcttttct gatactcatt acaggtgtta
     9721 atcatttttt tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacatag
     9781 aatctggttg tttcagtttc cgtttttatc tccccgatgg cgactatttc ggtggttaat
     9841 ttcaatttgg cggcatcgat atctttattc gcttgtttaa ccgtgtttga cagggtggta
     9901 aagaaattct gcacagattt taacttgttc ttcaattctg gaatcagttt cccttcaact
     9961 acgcccgcag caatagaata ggaaatgatt aatccaaatg gaccggcgac gacaccggca
    10021 tacgcttcct tcctgatttt atctacctgt gactggaaat agctgttttt ttctgaaaaa
    10081 tcattggtta actggctatc taacgccagc agtttcccgg aagcgttgtt gaaactttgt
    10141 gagcttacca gcagggattt ttgcgcttca ttcagcttcg tgatgccgtc atccagtacc
    10201 ttaatgagaa tgtctttctg ggcggatgct ttcttctcat tgtactcatc aaatagcaaa
    10261 atatacgctg cgagcaattg cgtcgcaaca ccaaaccatt catacactgt ttgggttgct
    10321 tcaaaatact tatcctggct atccataagt aaggttttaa tatcgccgac taaaacggag
    10381 gctgcctgtg aatactcctg tttaaagcga cttaactctt ttatggtttc atcaaaggtc
    10441 tgccagggga tgacctgatc gagatattta ttataaagat ctaatgctcc atctgcggtt
    10501 tcgattgcgt ttttaactac ttctaccgtt ttatctgcaa cgatttcagt cataatcatt
    10561 cgcctcttta aatatataaa ttgtaa
//
LOCUS       DAZFZC010000139        10573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFZC010000139 DAZFZC010000000
VERSION     DAZFZC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.140"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..1129
                     /locus_tag="ACHKXP_004445"
     CDS             56..1129
                     /locus_tag="ACHKXP_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7649312.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
     gene            1722..2573
                     /gene="hchA"
                     /locus_tag="ACHKXP_004446"
     CDS             1722..2573
                     /gene="hchA"
                     /locus_tag="ACHKXP_004446"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO7649313.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2681..4039)
                     /gene="hprS"
                     /locus_tag="ACHKXP_004447"
     CDS             complement(2681..4039)
                     /gene="hprS"
                     /locus_tag="ACHKXP_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO7649314.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNYSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4039..4710)
                     /gene="hprR"
                     /locus_tag="ACHKXP_004448"
     CDS             complement(4039..4710)
                     /gene="hprR"
                     /locus_tag="ACHKXP_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO7649315.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4843..5256
                     /gene="hiuH"
                     /locus_tag="ACHKXP_004449"
     CDS             4843..5256
                     /gene="hiuH"
                     /locus_tag="ACHKXP_004449"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO7649316.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5365..6369
                     /gene="msrP"
                     /locus_tag="ACHKXP_004450"
     CDS             5365..6369
                     /gene="msrP"
                     /locus_tag="ACHKXP_004450"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO7649317.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADLVASLYRGLDLRENF
                     "
     gene            6370..7005
                     /gene="msrQ"
                     /locus_tag="ACHKXP_004451"
     CDS             6370..7005
                     /gene="msrQ"
                     /locus_tag="ACHKXP_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO7649318.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            7262..7912
                     /gene="zinT"
                     /locus_tag="ACHKXP_004452"
     CDS             7262..7912
                     /gene="zinT"
                     /locus_tag="ACHKXP_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO7649319.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8255..8784
                     /gene="yodB"
                     /locus_tag="ACHKXP_004453"
                     /pseudo
     CDS             8255..8784
                     /gene="yodB"
                     /locus_tag="ACHKXP_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            9625..9713
                     /locus_tag="ACHKXP_004454"
                     /pseudo
     CDS             9625..9713
                     /locus_tag="ACHKXP_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(10129..>10573)
                     /locus_tag="ACHKXP_004455"
     CDS             complement(10129..>10573)
                     /locus_tag="ACHKXP_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NleF caspase inhibitor"
                     /protein_id="HFO7649320.1"
                     /translation="QDKLDVMISIYSNARNSNELEEIYQELSAFVSGLMDKRNSVFEV
                     RNENTDEVVGALREGMTIDDRDGYIRELFFLYSLKVKIEESRQGKEGSKCKVYGLLCP
                     HHSSELYGDLRAMKCLVEGCSDDFDPFDIIRVPDLTYNKGALQCG"
BASE COUNT         3049 a         2425 c         2136 g         2963 t
ORIGIN      
        1 gataacggca cttattgata tattcatgaa gattataatc acaagggaat acataatgaa
       61 aagaaaagtt ctggcaatgc tggtcccggc gttattagtt gctggcgcag caaatgcggc
      121 tgaaatttac aataaagatg gcaataaagt tgatttctac ggcaagatgg ttggcgaacg
      181 catctggtca aatactgatg ataataacag cgaaaacgaa gatacctcct atgctcgttt
      241 cggtgttaaa ggtgaaaccc aaatcaccag cgaactgact ggttttggtc agtttgaata
      301 caacctcgac gccagcaagc ccgaaggctc taatcaggaa aaaacccgtt taaccttcgc
      361 aggtttgaaa tataacgagt taggatcttt cgactatggt cgtaactacg gtgttgctta
      421 tgatgccgca gcttataccg atatgttagt tgaatggggt ggtgattcct gggcttccgc
      481 tgacaacttc atgaacggtc gtaccaacgg tgttgcaacc taccgtaact ctgatttctt
      541 tggtctggtt gatggtctga attttgctgt gcaatatcaa ggtaagaaca gcaatcgtgg
      601 cgttactaaa caaaacggtg atggctatgc gttgtctgta gactacaaca tcgaaggttt
      661 tggttttgta ggtgcatata gcaaatctga tcgtactaat gaacaagctg gtgacggcta
      721 cggtgataac gctgaagtgt ggtcattagc agccaagtat gatgcaaata atatctatgc
      781 agcaatgatg tacggtgaaa cccgcaacat gaccgttttg gctaatgatc attttgcaaa
      841 taaaacccaa aactttgaag ctgttgtaca gtatcagttc gacttcggtt tacgtccgtc
      901 tttaggctac gtatattcca aaggcaaaga tctttatgct cgtgatggac ataaaggtgt
      961 tgatgctgac cgcgtaaatt atatcgaagt tggtacctgg tactacttca ataagaacat
     1021 gaacgtctac acagcataca aatttaacct gctggataaa gacgatgcag cgattaccga
     1081 tgccgcaact gatgaccagt ttgcagttgg tatcgtctac cagttctaat cagccaacgc
     1141 gccgcggcgt gttactgtaa aacataacac tagcctgagt tcgtccctca tcgaactcag
     1201 gcttttttat tggcatttca tctccatctg tggcacacgc aattgccaaa gcatatcgat
     1261 acagcacctt accctgcccc tcctgccaca ataacatcta ccagcgactc ttccagccaa
     1321 caccccaaat gcacattctg atgaattacc ccgtaacaaa cacgcttccc aaatagaaat
     1381 ctttgcaaaa atagtgcatg aaataagctt tttctctacc aatccgcgat ataaatattc
     1441 atcagcacca caatattcca catcactaaa tctctcccca ccgccccgca tctctgataa
     1501 tggtctaaaa tcattgaggc cacttgcgac gacgttgctc aataaggaaa atttggcgtg
     1561 aataatattc aacaccagtg cagcatcatc attacatact tacacccgcc gactaccgct
     1621 ttcgatatat tgactcaatt ttccagcctg gttaaaaata gcgaccagct cagtcgcaaa
     1681 tatagtgact accctaacta agcaacaata aggaatacac tatgactgtt caaacaagta
     1741 aaaatccgca ggtcgatatt gctgaagata atgcgttctt cccttcagaa tattcgctta
     1801 gccaatatac cagtcccgtc tctgatcttg atggcgtgga ctatccaaaa ccgtatcgcg
     1861 gtaagcataa aattctggtg atcgccgcgg acgaacgtta tttgccaacc gataacggaa
     1921 aactgttctc gaccggtaac catccgattg aaacgttgct gccgttgtat catctccatg
     1981 ctgcaggttt cgaattcgaa gtggcgacca tttccggtct gatgaccaag tttgaatact
     2041 gggctatgcc gcacaaagat gaaaaagtaa tgccattctt tgagcagcat aaatcgttgt
     2101 tccgcaatcc gaagaaactc gcggatgttg tcgccagcct caacgctgat agcgaatatg
     2161 cagcaatctt tgttcctggt ggtcatggcg cacttattgg tttacctgaa agccaggacg
     2221 tagctgccgc tttacagtgg gcaatcaaaa atgaccgttt tgttatctcc ctttgccacg
     2281 gcccggcggc ttttctggcg cttcgccacg gcgataaccc actgaatggt tattccattt
     2341 gcgcattccc agacgccgca gacaaacaaa cgccagatat tggctatatg ccaggtcatc
     2401 ttacctggta cttcggcgaa gagctgaaga aaatgggcat gaacatcatt aatgacgaca
     2461 tcaccgggcg agtacataag gaccgtaaac ttctcaccgg cgacagtcct ttcgcagcga
     2521 atgcgttggg taaactggcg gcgcaggaaa tgctggcagc ttacgcgggt taatccctgt
     2581 tacgtttgaa tgtaagcgca taattaatcg cagccgcgtt agtactcacg acgtggctgc
     2641 gttttttcac ggttaattta tggcgtactg aagccctatg ttaatttctt tgcggtaacg
     2701 taattcggaa cacattatgc ttattgagat agtgatatga agcacttccc ccatgcaatt
     2761 cggcaatcgc tttgactaaa gaaagaccaa gtccctgacc tacggaatga cgcgaattat
     2821 ctccccgcca aaatctacgg aagagttttt caggctcatg tattttcgtt ccaggactgg
     2881 cgatatcaat attaagatag ccgttggtat caagaaaact ggttatatga atacgcgatt
     2941 tttctggtga atatctaatg gcattaacaa taagattcga taacattcgt tgcagtaaaa
     3001 ttttatccgc aaagatttgc tgattgcact cgaccttaaa gcaaatctct ttctcgtctg
     3061 aaagatattc aagataatcc aacaaatttt cgacttcctt attgagagaa agcgagtcca
     3121 gtttcaccaa aacattgttt ttatctgccc gggcaagaaa cagtatgttc tctgttaacc
     3181 gcgaaatatt ttccaactct tcaatgttac cggcaattgt tttttgatat tcagcgatac
     3241 ttctggtttg actgagcgta acctgattct gacccagtaa tgcattaatg ggcgttctaa
     3301 gttcatgagc gagatcgtca gcaaattgac ttaggcgttc aaaatctttg actaaggctt
     3361 gatgcatttt attcaacgcc tgcccaagag gttttagttc tctcggtaat gcgctaacct
     3421 caacaggctc tcggctatag ttataattca gcgcttccgt tacaccactc aactttttga
     3481 tctctcgtaa tcccgttctg attaacagcg gacttaatac tgagcaaagt acaatggcga
     3541 caatgcaaat tataatgcta ttaattttat actgttcaag catgttatgt ctggctgaag
     3601 ccaatttagc cacagtaacc gttaatgatg gcgaaacttc atcaatatta attcgtaaag
     3661 catcaatctc agtatcatta ataatgcttc tgtaaatgcc agctgcgctg attgtctcac
     3721 tagcaggtat gttatttaac atgtcatcac tgacatttgt ccggttaaca attttattga
     3781 tgccatcacc atgaatgatc aagatatcct gactaacatc catcatccgg ttaaagtata
     3841 caggtaacgt atctggattt accccatcaa ttaacaactg cttgatctgt gctgtccggt
     3901 taatgagtgt tgtatcatcg cgccatttca actcacttgc caggccatta tagagagtcc
     3961 agacaattcc agcgccagca acagacagca gcaatataaa aagcaaggtt aaacggacgg
     4021 ttatagacaa tcttttcatt atttttttac cgctacgaat gaatagccca tcccccggat
     4081 tgtggcgatt agcttttcag gaaaaggatc atcaactttt gcgcggagcc tgcgaatggc
     4141 gacatccacc gtattggtat cactatcaaa gttgattccc caaatttcac tcgcaataac
     4201 cgttctgggt ataatttcgc cagctctgga ggccagtagc caaagtaact gaaactcctt
     4261 gcgcgtcagt gtaatactga tattgtccct gctcacactt tgacttacag agtccattct
     4321 taagccactg atttctaatg ttgaattcaa agcgtgatgt tgccttaatt gtgcccgaac
     4381 ccttgccagc aactcagaaa atgaaaaagg ttttaccaga taatcatttg ctccactgtc
     4441 cagccctctg actctgtcat cgacagaatc ccttgcagta aggcaaataa caggggtttg
     4501 ctttgctgtt cttaacgttt gtaagatctg ccagccatcc atacccggaa gcataatatc
     4561 cagaattatc aatgcataat catccttcag cgcaagataa agcccatctc tgccatcaga
     4621 aacggcatca atgacataac ccgcttcgga aagcccctgc gttacccact cctgggtcct
     4681 ttgattatct tcaataagta aaatcttcat gaaaataata tgccataaat catatgttat
     4741 agaaacagcc tggttcatta caaaattgta atgctgctgt aaggttaccc tggccgcttt
     4801 ttcgctatcc tcaaaactca ttcacatgac aaggatataa acatgttaaa gcgttattta
     4861 gtactctcgg tagcaacggc agcattttca ttaccttctt tggtttatgc cgcacaacaa
     4921 aacattctta gcgtgcacat tttgaaccag caaaccggaa aacccgctgc tgacgtgaca
     4981 gtcactcttg aaaagaaggc ggataacggc tggttacaac ttaataccgc caaaacagat
     5041 aaggatggac gaattaaggc actgtggccc gagcaaactg caactacggg cgattaccgt
     5101 gtcgtattta aaaccgggga ctatttcaag aaacaaaatc ttgaaagttt cttccctgag
     5161 atccccgttg aatttcatat taataaagtg aacgagcatt atcatgtgcc tttattactt
     5221 agccaatatg ggtattcaac ctatcgtggc agttaatgca taaatatgta aaagcagatc
     5281 tctgctactg ttagggagga cactccctga cagattaaca gtaaacggct cttgctggct
     5341 aacgacaaaa aagtgtgatg gcttatgaaa aagaatcaat ttttaaaaga atcagatgtt
     5401 acggccgagt cggtattctt tatgaagcgt cggcaggtgt taaaagcact gggcatcagt
     5461 gcagctgcac tttctttgcc tcacgctgcg catgccgatc tgcttagctg gtttaaaggg
     5521 aacgatcgcc cgcccgcccc cgccggaaaa ccgctggagt tcagcaagcc tgccgcctgg
     5581 caaaataacc tgccactgac gccagtagat aaagtctccg gttataacaa cttctatgaa
     5641 ttcgggctgg ataaagccga tcccgccgct aatgctggta gcctgaaaac cgatccatgg
     5701 acactgaaaa tcagcggcga agtggcaaaa ccattgaccc tcgatcacga tgatttaacc
     5761 cgtcgcttcc cgctggaaga gcgtatttat cgtatgcgct gcgtggaggc atggtcgatg
     5821 gtggtgccgt ggattggttt tccgctgcac aaattgctgg cgcttgccga acccaccagc
     5881 aatgcgaagt atgtcgcttt cgaaacaatt tatgcaccgg aacaaatgcc aggccagcag
     5941 gaccgcttta tcggcggcgg gctgaaatat ccttatgtcg aaggattgcg tctcgacgaa
     6001 gcaatgcatc cgctcacact gatgaccgtg ggtgtttatg gcaaggcgtt accgccacaa
     6061 aatggcgcgc cggtacgact gattgtgccg tggaaatatg gctttaaagg gattaaatcg
     6121 atagtcagta ttaagctgac ccgcgagcgt ccgccaacca cctggaatct ggcagcgcct
     6181 gacgaatacg gtttttacgc caacgttaat ccgcatgttg atcacccgcg ctggtcacag
     6241 gctaccgaac gatttattgg ttcaggcggc atcctcgatg ttcagcgcca gccaacgcta
     6301 ctgtttaatg gttacgccga cctggtggca tcgctgtatc gtggcctgga tttgcgggag
     6361 aatttctgaa tgcgtctgac agcaaaacag gtgacatggc tgaaagttag cctgcatctt
     6421 gccggattgt tgccgtttct ctggctggtc tgggcgatca atcacggtgg actgggtgcc
     6481 gatccggtga aagatattca gcattttacc ggtcgcactg cactgaaatt tttgctggcg
     6541 accttgttaa tcacacctct ggcacgctac gcaaaacagc cgttattgat acgcactcgc
     6601 cgcctgttag gcttatggtg ctttgcctgg gcgacactgc atttaaccag ttacgcattg
     6661 ctggagctgg gcgtgaataa tctggcgtta ttaggcaagg agttaattac ccggccttat
     6721 ttaacgttag gaattatcag ctgggttatt ttgcttgctt tagcgtttac ttcaacccag
     6781 gcgatgcagc gaaaactcgg caagcactgg caacagttgc ataatttcgt ctatcttgtc
     6841 gcgatcctgg ccccaataca ttatctgtgg tcagtgaaga ttatttcacc gcagccgctc
     6901 atctacgctg ggctggctgt actgctttta gccttacggt ataagaagtt gcgttccctt
     6961 tttaaccggt tacgcaagca ggttcacaac aaattatctg tataacttca tattgccgac
     7021 aaagtacgcg ttatccctgc cggatgcggc gcgagcgccc tatccggcct acgcagatca
     7081 catcagatta tgtttcgttt gtcatcaacc tcaaattatc aatataattc attttatttc
     7141 aataagatag ataagtacac ctgagaaaac tatgctctca tctcctaaga gttgttgcat
     7201 tttgccatat gttacaatat aacattacac atcatataca atgactctgg aggaaactgt
     7261 tttggcgatt cgtcttcaca aactggctgt tgctttaggt gtctttattg ttagcgctcc
     7321 tgccttttcg catggtcatc actcacacgg caaaccctta acagaggtcg aacaaaaagc
     7381 tgctaatggt gtttttgatg atgccaatgt acaaaaccga acgctcagtg actgggatgg
     7441 agtctggcaa tccgtttatc ctttactgca aagtggcaaa cttgaccccg tctttcagaa
     7501 gaaagcggat gcagataaaa ctaaaacatt tgctgaaatt aaagattatt atcacaaagg
     7561 ttatgcaaca gatatcgaga tgattggcat tgaggacggc attgttgaat tccatagaaa
     7621 taatgaaaca acatcctgta aatatgatta cgatggatac aaaatactca cctataaatc
     7681 aggcaagaaa ggcgttcgtt acttattcga atgtaaagat cctgaaagca aagcccctaa
     7741 atatatacaa tttagcgatc atataattgc accacgaaaa tcatctcatt ttcacatttt
     7801 tatgggtaat gattcacagc aatctttatt aaatgaaatg gaaaactggc caacgtatta
     7861 tccatatcag ttgagtagcg aagaagtggt cgaggaaatg atgtctcatt gagtattctc
     7921 atgataacgc ctcgatgccg ctttagtaag tcatcataac tgccactggt ccatccacaa
     7981 acgccactga acgcaagcta gctacagaca cgctcatcac tatgacgtgt ctgtatatta
     8041 ataagctaac ccgcattgag ttaaccaata acggattcca tacacaatac ggccaacaga
     8101 gaaagatacc tgtgctcacg ccattgctta tattggctgg ttacaatgtg cactatcaat
     8161 tttttaaata acaaattctt aaaaccacat ctgtataagg aatttttaag gttcgtggga
     8221 tagcttgact gtaaaaatta caggagctac aaaaatgaac cgattctcaa aaactcaaat
     8281 ttatttacat tggataacgc tgcttttcgt tgcaataacc tatgccgcga tggaactccg
     8341 tggctggttt cctaaaggta gtagtactta tctgctgatg cgagaaacac attacaatgc
     8401 gggtatattc gtttgggtgt taatgttttc acgcctgatt ataaaacacc gttatagtga
     8461 tccttctatt gtgccaccgc cacctgcctg gcaaatgaaa gcggcttcgc taatgcacat
     8521 catgctttat ataaccttcc ttgcacttcc tctgctgggg attgctttga tggcttacag
     8581 tggaaaatcg tggagtttcc ttggtttcaa tgtttctccc tttgttaccc caaacagcga
     8641 aattaaagca ctgataaaaa tattcacgaa acctgggcaa atataggtta ctttttaatc
     8701 gcagctcacg ctggcgcagc actctttcat cactacattc agaaagataa tactctgtta
     8761 cgaatgatgc ctcgccgcaa ataagtcgat cccctgacaa ggaaatgtga accatggaca
     8821 tcaacgcgtc ataaatgttt acgcaagtgc acaatgcacc ctttccacta tcaacaagga
     8881 ggagcagaaa ctgtgtaaat cgtacaatgg taagacttat caaaaataca ggttaatttt
     8941 ggtatttaaa ccttttttac ttgttagtat gatacaaagg ctttcaaaaa agctgggaaa
     9001 ttcaacaact cttggaaatt atggactttg tcccgcggct ttaatctgac ggccgcgttc
     9061 cttttttgac tcaattattg aacccttctc tacgcaactt cagttcccac caccaacttt
     9121 gcggcagttt cataggatga atgtctaaaa gaataatggt gagcgataag aaaacgactg
     9181 aataactgca cattttcgct cgaaagcttc ctgtcagatc catagcgaat caagtgctga
     9241 atgtcacagt atcgaacaga aaacagtgac gatctaaccc ttcaagaata ttctacgatt
     9301 gttctgttta ggaaaagcaa ggcgggaagt cgggagataa gtcattgata aagtggcgga
     9361 gagaggggga tttgaacccc cggtagagtt gcccctactc cggttttcga gaccgatgat
     9421 accgaatcgc aaaatcaaaa ggttataaat ttttcttgga ataaatgtct taaaaacata
     9481 cacgaaaaat caataaacta cgttgaattc aataagaata ttccaagcct tctttgagtt
     9541 atttttgacc agtttgctgt ttttctttct cataaaatat ttcctgatat cccttaacat
     9601 tactgatact caagtagtga atttatgagc aatcctaatc catgcatgac gtgtggtgcc
     9661 tgttgtgcat ttttcgcgtc tctttttact gggctgaagc agatgatgct ggcgaaaggt
     9721 ttatcagaaa accgtagcgc cttcttgcat acgacgtgtc cgcagtttta tataatcccg
     9781 accagaaacc tcttataaaa agtcgataca caaatatcat ggataatcac caccttctga
     9841 cgaagaatat ctgatgaaat taatcgcccc tctcctgaga ccattgcgct ggggtaaatt
     9901 tgtaactacg tgtgtacagt gaggtgtcgt atagcacgcc gtcaggaaat gcaaggtgcc
     9961 cccgatatcc ccgactacaa gtcagcaaac aaagtgattg gctggcaatc cacatcacag
    10021 aaatggacaa tgctacttta taatcgagct tggtagccac cttgatggtc acttttaaaa
    10081 aaagcgttta cacaaaatga taagcaggct cacaggacct ccctctaatc atccacattg
    10141 taaagctcct ttgttgtaag taagatccgg caccctaata atatcaaaag gatcaaaatc
    10201 atcactgcat ccttccacaa gacatttcat tgctcgtaga tcaccatata gctctgaaga
    10261 gtgatgcgga cataacagac cataaacttt acatttagaa ccttctttac cttgtctact
    10321 ttcctcaatt tttactttca atgaatacag aaaaaaaagc tccctgatat aaccatccct
    10381 gtcatctatc gtcatcccct ccctcagtgc tccgacaacc tcatcagtat tttcatttct
    10441 cacctcaaat acactatttc tcttatccat cagcccactt acaaaagcac ttagctcttg
    10501 ataaatttcc tcaagctcat tactatttct ggcatttgag tatatagaga tcataacatc
    10561 aagtttatct tgt
//
LOCUS       DAZFZC010000140        10436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFZC010000140 DAZFZC010000000
VERSION     DAZFZC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.141"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..985)
                     /locus_tag="ACHKXP_004456"
     CDS             complement(<1..985)
                     /locus_tag="ACHKXP_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649321.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLAELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRSDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGVSGHNDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNTFKENLFFVEIVSCCFLVELFHDIDSFFINQRLSKLESFIDKWREGKEIW"
     gene            1170..2054
                     /locus_tag="ACHKXP_004457"
     CDS             1170..2054
                     /locus_tag="ACHKXP_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7649322.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDMPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDDAVARFSFPAWMLQLLR
                     KTRDRIIHLLVTLWDHLF"
     gene            2173..2850
                     /locus_tag="ACHKXP_004458"
     CDS             2173..2850
                     /locus_tag="ACHKXP_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649323.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            2856..3089
                     /locus_tag="ACHKXP_004459"
     CDS             2856..3089
                     /locus_tag="ACHKXP_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7649324.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            3179..3997
                     /locus_tag="ACHKXP_004460"
     CDS             3179..3997
                     /locus_tag="ACHKXP_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7649325.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            4079..4558
                     /locus_tag="ACHKXP_004461"
     CDS             4079..4558
                     /locus_tag="ACHKXP_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7649326.1"
                     /translation="MKSVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            4574..5050
                     /locus_tag="ACHKXP_004462"
     CDS             4574..5050
                     /locus_tag="ACHKXP_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7649327.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            5113..5334
                     /locus_tag="ACHKXP_004463"
     CDS             5113..5334
                     /locus_tag="ACHKXP_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7649328.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            5414..5788
                     /locus_tag="ACHKXP_004464"
     CDS             5414..5788
                     /locus_tag="ACHKXP_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7649329.1"
                     /translation="MSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            5835..6212
                     /locus_tag="ACHKXP_004465"
     CDS             5835..6212
                     /locus_tag="ACHKXP_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7649330.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            6209..6697
                     /locus_tag="ACHKXP_004466"
     CDS             6209..6697
                     /locus_tag="ACHKXP_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7649331.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRASAERTPPSAALTGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            6709..6906
                     /locus_tag="ACHKXP_004467"
     CDS             6709..6906
                     /locus_tag="ACHKXP_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7649332.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            6991..7857
                     /locus_tag="ACHKXP_004468"
     CDS             6991..7857
                     /locus_tag="ACHKXP_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7649333.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LTEISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYENNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     ALASTVNKSRKA"
     gene            7929..8192
                     /locus_tag="ACHKXP_004469"
     CDS             7929..8192
                     /locus_tag="ACHKXP_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7649334.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            8189..8515
                     /locus_tag="ACHKXP_004470"
     CDS             8189..8515
                     /locus_tag="ACHKXP_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7649335.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            8979..10244
                     /locus_tag="ACHKXP_004471"
     CDS             8979..10244
                     /locus_tag="ACHKXP_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7649336.1"
                     /translation="MALTDAKIRAAKPSDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKENTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKFISPFEYAKINNPLKQ"
BASE COUNT         2732 a         2676 c         2459 g         2569 t
ORIGIN      
        1 tccagatttc tttaccctcc ctccacttat ctataaaact ttccagctta cttaatcgtt
       61 gatttataaa aaacgaatca atatcatgaa aaagctcaac aagaaaacag cacgagacaa
      121 tctcaacaaa aaacaaattc tccttaaaag tattataaaa ccttatcaac acataacgaa
      181 aaacacctcc tgttaatctt tcctgttcat aaaacaacaa accaaaaaca ggaggaaatc
      241 ttttaaccac accactttta atccagtaag gatgatgatt agtctttact ggcagtgaat
      301 aaaagaacaa ttcggtttct cttttcccac cacaaacaga caaaactgtc ttataaagac
      361 tttttgaatc attatgtcct gataccccag acgaattagc agagtatttt ctttttactt
      421 tttgccttaa tggagctgga agaataataa aacgggatgg cagataacaa tcgctttcag
      481 aagaccggca ttcgcacaca tgaagattta tatttacatc cggacaattc aaaaaccaca
      541 cagaataatt atcgctccgt ttgcaatatg aaacccatga ataaattgca taaagttcag
      601 aaagtacatc taactctgct tttgatttaa atgtgatacc gtttcttcga attctcgttt
      661 ccagcgttct ccatgccgga gttttcagta tctcacgaac ttttttatta agttcagcca
      721 gcgatggtcc ctgctcctgt ttatgtttct tcctgctgtt aatgatttct ctttctttta
      781 ctaatttttt cttacttttg ggtcctttta tctttcttgg caatgcttca tttaatctgt
      841 gaacaggatt ttgctctctt ctttttttga gctcacaaaa cctctcataa tgttttctgc
      901 acagtctttt ctccactgcc atttttactg agaaaacctg attgttacaa ttttttctga
      961 cgcatttgtt tttacgtccg gtcatcctct catctcctga aaccgcagta tgtcattagt
     1021 tcccgttcgg aaaaactcac ttcatactcc attacacttt aacaatcatg tccgtaattc
     1081 agacattcca tcatttttca cgtccatatt acctgaatga taaaccggat tagtgtgatc
     1141 cggttcagcg aaatcaacag gataccggta tgtcattcag ccattcttcc ctctctgcac
     1201 aagtaaaatc atatctgacg tttcttcctg aagaaattcg ccagaaaatc cttgaacatc
     1261 tccacggtgt tattcattac gagcccgtaa ttggcattat gggtaaatcc ggcaccggca
     1321 aaagcagtct gtgtaatgcc atttttcagt cccgtatctg cgccacgcat cccctgaacg
     1381 gctgcacccg ccaggctcat cgtcttaccc tgcagctcgg tgaacgcaga atgacgctgg
     1441 tcgatatgcc cggcattggt gagacaccgc agcatgatca ggaataccgg gagctttacc
     1501 gtcagttact gccggaactg gatctgatta tctggatcct gcgggctgat gaacgtgcgt
     1561 atgctgctga tattgccatg catcagtttt tactgaatga aggtgcagat ccctcgcgtt
     1621 ttctgtttgt tctcagccat gccgatcgcg tgtttcctgc tgaagaatgg aacgacacag
     1681 aaaaatgccc gtcccgtcag caggaactct cactggcgac agtaacagcc cgggtggcaa
     1741 ccctgttccc gtcatcattt ccggtactct ccgttgccgc acctgtcggc tggaatcttc
     1801 cggcactggt gtcgctgatg atccacgcac tgccaccaca ggcaaccagc gcagtttatt
     1861 cacatatccg gggggaaaac cgctctgaac aggcccggaa acacgcacaa cagacttttg
     1921 gtgatgccat cgggaaaagt tttgacgacg ccgttgcccg tttcagtttt ccggcctgga
     1981 tgttacagct tctgcgcaaa acccgggacc gcattatcca cctgctggtc acgctgtggg
     2041 atcacctgtt ctgaacgccc cggagtcccc cgtggcaacc gtataccttt acacgacaac
     2101 ggattacggt aaccactgcc gtataccgtc tgtcattatc aataacacgc tctttaattt
     2161 cagaggtcag tcatgaatac cagaattctt tttcccctgc tgttcactgt ggcatcattc
     2221 tccgcctccg ccggcaactg ggctgtcaaa aacggctggt gtcagaccat gacggaagat
     2281 ggtcaggcgc tggtaatgct gaaaaatggc acgattggta ttactggcct gatgcaggga
     2341 tgcccgaatg gtgtacagac gctcctgggc agccgtatca gtattaacgg taacctgatc
     2401 cccacatcac aaatgtgtaa tcagcagacg ggattcaggg ctgttgaggt ggaaatcgga
     2461 caggcgccgg aaatggtcaa aaaagccgtt cactccatag cagagcgtga tgtgtccgtt
     2521 ttacaggcat ttggtgtacg aatggaattc acccgcggtg atatgctgaa ggtctgtccg
     2581 aaatttgtca catcacttgc cggtttttcc ccgaaacaga cgaccactat taataaagat
     2641 tccgtcctgc aggctgcccg gcaggcatac gcccgggaat atgacgagga aacaacagaa
     2701 accgctgatt ttggctctta cgaagtaaaa ggcaataagg ttgagtttga agtattcaat
     2761 cctgaagacc gtgcgtacga caaagtgacc gtcacggttg gtgctgacgg taatgccacc
     2821 agcgccagcg ttgaatttat cggaaaatag tcggtatgtc ggactgccac cctgttttat
     2881 tgcccgaagg ccctttctca cgcgaacagg cggttgccgt cacaacagct taccgcaatg
     2941 tgcttattga agatgaccag ggaacgcatt tccggctggt tatccgcaat gccgaagggc
     3001 agctacgctg gcggtgctgg aattttgaac ctgatgccgg aaaacagcta aattcgtatc
     3061 tcgccagtga gggaattctc aggcaataaa cgtcttcatt tcatccatca ggccgcgtct
     3121 tctccgggag acgcggcttt ttcgtttata ccgctaattc attcataagg agcaaagtat
     3181 gcaattagcc agtcgttttg gtcatgtaaa tcagatccgt cgggagcgcc cactgacacg
     3241 cgaagaactg atgtaccacg tcccgagtat ttttggagaa gaccggcaca cctcccgcag
     3301 tgaacggtat gcgtacattc ccaccattac cgtcctggaa aatctgcagc gggaaggctt
     3361 tcagccgttc ttcgcctgcc agacccgtgt gcgcgaccag agccgccggg aatataccaa
     3421 acacatgctg cgtctgcggc gggccggaga gataaacgga gaacatgtcc ctgaaattat
     3481 tctgctcaac tctcatgacg gtacctccag ctaccagatg ctgccgggtt acttcaggtt
     3541 cgtctgccag aacgggtgcg tctgtggcca gtctctgggg gaagtgcgtg ttccgcaccg
     3601 gggaaatgtg gtggacagag tcattgaagg ggcttacgaa gtggtgggcg tgtttgaccg
     3661 gatagaggag aagcgtgatg ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc
     3721 gctggcacag gcggcactga cttaccgtta tggtgacgaa catcagcccg tcaccaccgc
     3781 cgacattctg acgccgcgac gccgggagga ttacggtaag gacctgtgga gcgcatatca
     3841 gaccatccag gagaatatgc tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg
     3901 tatccatacc cgggccattc acagcatcga caccgatatt aaactcaacc gcgcattgtg
     3961 ggtgatggca gaaacgatgc tggagagcct gcgctgatgc cgtttccctg agtgagggaa
     4021 tcattcagct ttcgttcctg aaaaacacac ttttatttgt tatttaagga gttatctcat
     4081 gaaaagcgtt tctcagaata cccccacaat ttattcagct acaacaccag agaataatcc
     4141 gcctcagttg gttgccagcc tcgtccctga tgaacagcgc atcagctttt ggccgcagca
     4201 ttttggcctc attccacagt gggtgaccct ggagccccgt gtcttcggct ggatggaccg
     4261 tctgtgtgaa gactactgcg gtggtatctg gaatctgtac accctgaaca acggtggcgc
     4321 atttatggca cccgaaccgg atgacgatga tgacgaaaca tgggtgctgt ttaacgtcat
     4381 gaacggtaac cgcgctgaaa tgagcccgga agccgccggc attgccgcct gtctgatgac
     4441 gtacagtcat cacgcctgtc gtacggagaa ttatgccatg acggtccatt attatcggtt
     4501 gcgggattac gccctgcagc atccggaatg cagcgccatt atgcgcatca tcgactgaat
     4561 ggagagaagc ataatgcaac agctttcctt tctgcccgga gcgatgacgc ccggcgagcg
     4621 cagcctcatt cagcgggccc tgaaaaccct ggaccgtcat cttcatgagc ccggcgtggc
     4681 cttcacctcc acgcatgcgg cacgggaatg gctgattctg aacatggcag gactggagcg
     4741 ggaagagttc cgggtgctgt atctgaacaa ccagaatcag ctgattgcgg gtgaaaccct
     4801 cttcacaggc accatcaacc gcacagaagt ccatccccgg gaggtgatta aacgtgccct
     4861 gtaccacaat gccgctgccg tggtgctggc gcacaatcac ccgtccggtg aagtcacccc
     4921 cagtaaggca gaccggctta tcactgaacg tctggtacag gcgctggccc tggtggatat
     4981 ccgggtgccg gaccatctga tagtcggtgg caaccaggta ttctcctttg ccgaacacgg
     5041 tctgctttaa cccgtcacaa ccacatcaca cctgttttca cttttatctt ctgtcttcag
     5101 aggtatccca ttatgaaaat catcacccgt ggtgaagcca tgcgtattca ccaacagcat
     5161 cctgcatccc gtctttttcc gttctgtacc ggtaaatacc gctggcacgg tagcacggat
     5221 acgtataccg gccgtgaagt gcaggatatt cccggtgtgc tggccgtgtt tgctgaacgc
     5281 cgtaaggaca gttttggtcc gtatgtccgt ctgatgagcg ttaccctgaa ctgaatcaga
     5341 aagggcattc tgatgagccg aattatcgcc accatcggag cattcttaac cgattttctg
     5401 tgaggatttt atcgtgtcag acacacaccc cgggacaacg catcccgaca acaacaacga
     5461 ccgcccctgg tgggggctcc cctgcaccgt gacgccctgt ttcggggcac gtctggtgca
     5521 ggagggcaat cagttgcatt accttgccga ccgcgccggt atcagggggc agtttagcga
     5581 cgcggatgcg tatcatctgg accaggcctt tccgctgctg atgaaacaac tggaactcat
     5641 gctcaccagc ggtgaactga atccccgcca tcagcatacc gtcacgctgt atgccagagg
     5701 gctgacctgc gaagccgaca ccctcggcag ctgtggttac gtttatatgg ctgtttatcc
     5761 gacactggca cccgcaacca cctcataacc ccgaatatct cacctttagc actaccagtt
     5821 acagagaact taagatgaaa acattacctg acacacacgt acgggaggca tcgcgctgcc
     5881 cgtctcccgt caccatctgg cagacactgc tcacccgact gttggaccag cattacggcc
     5941 tcacactgaa tgacacaccg ttcgctgatg aacgtgtgat tgagcagcat attgaggcag
     6001 gcatttcact gtgcgatgcg gtgaactttc tcgttgaaaa atacgcactg gtacgtaccg
     6061 accagccggg attcagcgcc tgtacccgtt ctcagttaat aaacagtatt gatatcctcc
     6121 gggcccgccg ggcaaccggc ctgatggccc gcgacaatta cagaacggta aataacatta
     6181 ccttgggtaa acatccgggg gcgaaacaat gaaactggcc ctgacgctgg aagccgacag
     6241 cattaacgtg caggcactga acatggggcg cattgtcgtt gacgtcgatg gtgttactct
     6301 cgctgaactg attaacgtgg tctgcgataa cggttactcc cttcgtgttg ttgatgaatc
     6361 tgaccgggca tcagcagagc gcacgccacc atctgcggcc cttaccggca tacgctgcag
     6421 taccgcacat atcacggaaa cggacaatgc ctggttgtac tcgctgtcac atcagacgaa
     6481 tgacaccggc gaatcagaat ggattcattt tacaggtagc ggatatctgt tacgtaccga
     6541 tgcgtggtca tacccggttc tgcgacttaa acgcctggga ctgtcaaaaa cgttccgccg
     6601 tctggttgtc actcttatcc ggcgttatgg cgtcagtctc attcatctgg atgccagcgc
     6661 tgaatgcctg cctgacctgc ccactttcga ctggtaacca ggaacaacat gaaatcatta
     6721 accacggaaa cagcactgga tattctgatt gtgtggctgc aggacaatat cgactgcgaa
     6781 tcgggaatta tctttgacaa cgatgaggat aaaacggatt cggcagcact gttgccctgt
     6841 atcgaacggg cccgggagga tgtccgtact cttcgtcatc tgcagcttct gcaccagaac
     6901 cggtaaatat tactcaccag acttcattcc actgacgcca gcctgaacag ggctggcgtt
     6961 ttcatttatc tgcaaaaagg aatatcgatt atgtctgata tcacagcctc ccgtccggaa
     7021 gtggtcaacg ggcatacgga cgttatctgc tcaaccagta tccgccacat tctggctgta
     7081 cgaaagagta cactgctgca aatcgacaca cttatccggc aactgactga aatctcagtt
     7141 ctgacggaaa gcattggagg taaaaccgcc cgggactggg ccatgaaaca ggattttcgc
     7201 tgtggctgct ggctgatgga taaaccggaa acagcaatga aagccatcac ccgcaatctc
     7261 gatcgcgaaa tctggcgtga cctgatgcaa cgttccggga tgctgtcatt aatggatgcg
     7321 caggcccgtg atacatggta ccggtcactg gaatacgata attttccgga aatcagtgaa
     7381 gcgaacattc tgagcacatt tgaacaactg caccagaaca aagatgaagt gtttgagcgt
     7441 ggggtgatca atgttttcag gggactgagc tggaattacg aaaacaattc accctgtaaa
     7501 tttggcagta aaattatcgt caacaacctg gtgaggtggg accggtgggg gtttcatctt
     7561 atcaccgggc aacaggcaga tcgacttgcc gacctggaaa gaatgctgta tctgttcagc
     7621 ggcaaaccga tccccgacaa ccgggaaaac atcaccattc atctggatga ccacatccag
     7681 tctgttcagg gtaaagagga ctatgaagat gagatgttca gcatcagata ctttaagaag
     7741 ggctccgcgc acatcacgtt caggaaacca gaactggttg acaggcttaa tgatattatt
     7801 gccagacact actcggaagc cctggcaagc acagtgaaca aaagccgcaa ggcataaaag
     7861 aggtatttta caattcgcga atattaacta ttattaattg atagtaatca atcggcgaga
     7921 aaatcaccat gcctaccagt gtatccctca gtccttactt tgaaacgttt atccgcgaac
     7981 aaatagaaag tggtcggtat aacaatacaa gtgaagtcat tcgtgccgga cttcgtgctc
     8041 tggaagaacg ggaacagcag ataaagcttg aatcactcca gagcgccgtt accgcaggca
     8101 tcaacagcgg tgaaagtaaa agtgctgaag aggtattcgg gcgactgacg cacaaataca
     8161 aaaagatggc agaaggtgaa cagccaatat gaaactgact gtttcaccat tagcagaaca
     8221 cgatcttgaa gctataggtg actggatcgc tcaggataat cctgtccgtg caatcagttt
     8281 taccgaagag ctttaccaac aatgtctgtt gatagccgaa tcgccagtac tctacagaga
     8341 aagacccgag ttaggggcag ggatcagagg ttgttcgtat ggtcgttacc ttctgctatt
     8401 cagggtactg gatactgaag ttcgtatcga acgtatcgtt catggttccc gggatatcaa
     8461 cagggtactt gcagagccag acgacaaacc atcagatcat ttctggcagc cataaacaaa
     8521 atctgtgcgt taacgctccc taacacaggt agccaggtat tttataaatc tctgtatttt
     8581 gttgttactt cgattccgcg attcagcata tcgaaccaga caaaatgatt acaacaaagc
     8641 acaaaaaaga gggattatag cgtcaaaaga attcgaattg cacatggggg cctcattggt
     8701 ggccccaaaa agaacaagga taaaacagtc gatataaaac aattggttat ataaccagtt
     8761 tcattccgag tccgggcacc aaattcatat caacggacct ccacggaggt ccgtttttcg
     8821 tttcagaaca ccatgattta agtgttcttc ctccaaatca attctaccga actcaaccaa
     8881 attctcccca catcaacctc attgtgtggg tataattgcg ggcatacccc agttcgacag
     8941 aatttgtacc ctctttaacg cattcagaag gctgaactat ggcactgact gacgcaaaaa
     9001 tccgggctgc aaagccctct gacaaggctt ataaactcac tgacggggct ggcatgttcc
     9061 tgctggttca tcctaatggt tcccgttact ggcgtctccg ttatcgtatt ctgggtaagg
     9121 agaatactct ggcacttggt gtgtatccag aagtttctct ctccgaagct cgtacaaaac
     9181 gggatgaggc ccgaaaactg atttcagagg ggattgaccc ttgcgaacag aaaagagcta
     9241 aaaaagtagt ccctgattta caactctctt ttgaacatat tgcacgacgc tggcatgcca
     9301 gtaataaaca atgggcacaa tcacacagcg ataaggtact caaaagcctc gaaacacacg
     9361 ttttcccctt tatcggcaac cgggatatca caacactcaa taccccggac ctgcttatcc
     9421 ctgttcgtgc tgcagaagct aaacaaattt atgaaatcgc cagtcgtctg cagcaaagaa
     9481 tatctgctgt aatgcgttat gctgtacagt ctggcatcat cagatataac ccggctctgg
     9541 atatggctgg tgcattgacc acggtaaaac gccagcatcg ccctgctctg gatctttcac
     9601 gcctgcctga acttctgtcg cgtattgacg gttataaagg ccagcctgtc acccgactgg
     9661 cagttatgct gaatttactg gtttttattc gttccagtga actcagatac gcccgctggt
     9721 ctgaaattga tattgacaat gccatgtgga ctattccagc cgaacgcgaa cctctgccag
     9781 gcgtaaaatt ctcacaccgg ggctccaaga tgcgaacacc acatcttgtg ccactcagca
     9841 aacaggctgt agccatactg acagaacttc agacatgggc aggtgaaaat ggtctgatat
     9901 ttacgggtgc acatgacccg cgtaaaccaa tcagtgaaaa tactgtaaat aaagccctga
     9961 gggtgatggg gtatgacaca acccaggaag tctgtggcca tggattccgg gcgatggcgt
    10021 gcagtgcatt gattgaatca ggtttgtggt cccgcgatgc tgtggaacgt cagatgagcc
    10081 atcaggaacg taacggtgta cgtgctgctt acattcataa agcagaacat ctggaagaac
    10141 gccgcttgat gctacaatgg tgggccgatt ttctggatgc aaacagagaa aaatttatca
    10201 gtccatttga atatgcaaag attaataatc cattaaaaca gtaatcatcc cgggcaaatg
    10261 cccgggaacc tactccagga ttattttctt tgttaaaaaa acagacaaaa cggtattaac
    10321 ttcgtaagcg tacagcgagg gccgtattga cggggatgtg ttattcagct ggcagtgcta
    10381 tgcgccacgg aagcagttcg ctgacccggt tgaccggcca gtctgctatg acgcca
//
LOCUS       DAZFZC010000141        10313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFZC010000141 DAZFZC010000000
VERSION     DAZFZC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.142"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            119..694
                     /gene="ssuE"
                     /locus_tag="ACHKXP_004472"
     CDS             119..694
                     /gene="ssuE"
                     /locus_tag="ACHKXP_004472"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO7649337.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            687..1646
                     /gene="ssuA"
                     /locus_tag="ACHKXP_004473"
     CDS             687..1646
                     /gene="ssuA"
                     /locus_tag="ACHKXP_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO7649338.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1643..2788
                     /gene="ssuD"
                     /locus_tag="ACHKXP_004474"
     CDS             1643..2788
                     /gene="ssuD"
                     /locus_tag="ACHKXP_004474"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO7649339.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLQRETVDFNGKHIHVRGA
                     KLLFPAIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPLLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2799..3590
                     /gene="ssuC"
                     /locus_tag="ACHKXP_004475"
     CDS             2799..3590
                     /gene="ssuC"
                     /locus_tag="ACHKXP_004475"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO7649340.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3587..4354
                     /gene="ssuB"
                     /locus_tag="ACHKXP_004476"
     CDS             3587..4354
                     /gene="ssuB"
                     /locus_tag="ACHKXP_004476"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7649341.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4397..7009)
                     /gene="pepN"
                     /locus_tag="ACHKXP_004477"
     CDS             complement(4397..7009)
                     /gene="pepN"
                     /locus_tag="ACHKXP_004477"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO7649342.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QNGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYLFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7275..8477
                     /gene="pncB"
                     /locus_tag="ACHKXP_004478"
     CDS             7275..8477
                     /gene="pncB"
                     /locus_tag="ACHKXP_004478"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO7649343.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8646..10046
                     /gene="asnS"
                     /locus_tag="ACHKXP_004479"
     CDS             8646..10046
                     /gene="asnS"
                     /locus_tag="ACHKXP_004479"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO7649344.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2332 a         2640 c         2776 g         2565 t
ORIGIN      
        1 ttattttcag aaaaagatac acaaaattta tatttggcat tttcttgtct ctccgacaga
       61 ctgaccgaat tgttatcaat gttaacaaaa aaagaacaat tggttataag gagagagtat
      121 gcgtgtcatc accctggcgg gtagtcctcg ctttccttct cgctccagct ccttgctgga
      181 atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt tatcactgga atctgcaaaa
      241 cttcgccccg gaagatctgc tttatgctcg tttcgatagt ccggcactca agaccttcac
      301 cgaacagctg caacaggctg atggactgat agtcgctaca cctgtgtata aagccgccta
      361 ttccggcgcg ctgaaaaccc tgctcgacct gctgccagag cgcgctttgc aaggcaaagt
      421 ggtgctaccg ctggcgacgg gcggtaccgt ggcccatctg ctggcggtcg attatgccct
      481 taaaccagtt ttaagcgcac tgaaagctca ggagatcctg cacggcgtgt ttgccgatga
      541 ctcacaagta attgattacc atcacagacc ccagttcacg ccaaatctgc aaacccgtct
      601 tgataccgcg ctagaaactt tctggcaggc attgcaccgc cgcgatgttc aggttcctga
      661 ccttctgtct ctgcgaggta atgcccatgc gtaacatcat taaactggcg ctggtgggat
      721 tgcttagcgt ctctacgatt gcggttgctg cagaatcctc gcctgaagcg ttacgtatag
      781 gctatcagaa aggcagtatt ggtatggtac tggcaaaaag ccaccagtta ctggaaaaac
      841 gctatccgca atcaaaaatc tcctgggtgg agttccccgc gggtccgcaa atgttggaag
      901 cgttaaacgt tggcagtatt gatctcggca gtaccgggga tattccgcca atctttgccc
      961 aggctgccgg ggctgatttg gtatatgtgg gcgtcgagcc accgaagccc aaagccgaag
     1021 tgattctggt ggcagaaaac agcccgatca aaaccgtagc cgatcttaaa ggtcacaaag
     1081 ttgcctttca gaaaggttcc agttcacaca accttttact gcgtgcactg cgtcaggccg
     1141 gacttaagtt taccgatatc caacccactt acctgacgcc cgctgatgcc cgcgccgcgt
     1201 tccagcaagg taacgttgac gcctgggcta tctgggatcc ctactactct gctgcattat
     1261 tacagggcgg cgtgcgggtg ctgaaagacg gcaccgatct caatcaaact ggatcgtttt
     1321 atctggcagc tcgtccctat gcagaaaaaa acggcgcttt tattcagggc gtactggcaa
     1381 cctttagtga ggccgatgcg ttaacccgca gccagcgcga gcaaagcatc gctttactgg
     1441 caaaaacgat gggcttaccg gcaccggtga ttgcctctta cttagatcat cgccctccta
     1501 ccaccatcaa accggttaac gccgaggttg ccgccttaca gcagcaaacg gcagatctgt
     1561 tttatgaaaa tcgtctggtg ccgaaaaaag tcgatattcg ccagcgcatc tggcagccca
     1621 ctcaactgga aggaaaacaa ttatgagtct gaatatgttc tggtttttac cgacccacgg
     1681 tgacgggcat tatctgggaa cggaagaagg ttcacgcccg gttgatcacg gttatctgca
     1741 acaaattgcg caagcggcgg atcgtcttgg ctataccggt gtgctaattc caacggggcg
     1801 ctcctgcgaa gatgcgtggc tggttgccgc atcgatgatc ccggtgacgc agcggctgaa
     1861 gtttcttgtc gccctgcgtc ccagcgtaac ctcacctacc gttgccgccc gccaggccgc
     1921 cacgcttgac cgtctctcaa atggacgtgc gttgtttaac ctggtcacag gcagcgatcc
     1981 acaagagctg gcaggcgacg gagtgttcct tgatcatagc gagcgctacg aagcctcggc
     2041 ggaatttacc caggtctggc ggcgtttatt gcagagagaa accgtcgatt tcaacggtaa
     2101 acatattcat gtgcgcggag caaaactgct cttcccggcg attcaacagc cgtatccgcc
     2161 actttacttt ggcggatcgt cagatgtcgc ccaggagctg gcggcagaac aggttgatct
     2221 ctacctcacc tggggcgaac cgccggaact ggttaaagag aaaatcgaac aagtgcgggc
     2281 gaaagctgcc gcgcatggac gcaaaattcg tttcggtatt cgtctgcatg tgattgttcg
     2341 tgaaactaac gacgaagcgt ggcaggccgc cgagcggtta atctcgcatc ttgatgatga
     2401 aactatcgcc aaagcacagg ccgcattcgc ccggacggat tccgtagggc aacagcgaat
     2461 ggcggcgtta cataacggca agcgcgacaa tctggagatc agccccaatt tatgggcggg
     2521 cgttggctta gtgcgcggcg gtgccgggac ggcgctggtg ggcgatggtc ctacggtcgc
     2581 tgcgcgaatc aacgaatatg ccgcgcttgg catcgacagt tttgtgcttt cgggctatcc
     2641 gcatctggaa gaagcgtatc gggttggcga gttgctgttc ccgcttctgg atgtcgccat
     2701 cccggaaatt ccccagccgc agccgctgaa tccgcaaggc gaagcggtgg cgaatgattt
     2761 tatcccccgt aaagtcgcgc aaagctaagg aagaaataat ggcaacgcca gtgaagaagt
     2821 ggttattgcg cgttgccccc tggtttttac cggtgggcat cgtggcggtg tggcaactgg
     2881 cctcctcggt tggctggctg tcgacgcgta ttttgccttc accggaagga gtggtgacgg
     2941 cgttctggac gctctccgcc agcggcgaac tgtggcagca tctggcgatc agctcctggc
     3001 gggcgctgat tggcttttca attggcggat cgctggggtt gattttggga ctgattagcg
     3061 ggctatctcg ctggggagaa cggctgctgg atacctcaat tcagatgttg cgcaacgtgc
     3121 cgcatctggc gctgatcccg ctggtgattt tgtggtttgg cattgatgaa tccgcaaaaa
     3181 tctttctggt ggcgctcggt acgctattcc ccatttatat caacacctgg catggcatcc
     3241 gtaatatcga tcgcgggttg gtggagatgg cgcgtagcta tggattatcc ggcataccgc
     3301 tgtttatcca tgtgatcctg cctggtgccc tgccctcaat tatggtcggc gtgcgttttg
     3361 cgttgggcct gatgtggctg acgctgattg ttgccgaaac catttctgcc aattcaggca
     3421 ttggttatct ggcgatgaat gcgcgggagt ttttgcaaac ggacgtggtg gtagtcgcta
     3481 ttattcttta cgccctgctc ggcaaactgg ctgacgtcag cgcgcagctg ttagagcgcc
     3541 tctggttgcg ctggaacccg gcttatcatt tgaaggaggc cacggtatga atactgctcg
     3601 tctgaaccag ggcacgccat tgttgctcaa tgcagtaagc aaacattacg cggaaaatat
     3661 cgtcctgaac caactggatt tacatattcc ggcaggtcag tttgtggcgg tggtgggccg
     3721 cagcggtggt ggcaaaagta ccctgctgcg cctgctggca ggtctggaaa cgccaaccgc
     3781 aggcgatgtg ttagcgggca ccacaccgct ggctgaaatt caggaagaca cgcgaatgat
     3841 gtttcaggat gcgcgtctgc taccgtggaa atcggtgatt gataacgttg ggttaggcct
     3901 taaaggccag tggcgcgatg ccgcacgtcg agcgttggct gcggtaggac tggagaatcg
     3961 cgcaggggaa tggcctgccg cactttctgg cgggcagaaa cagcgagtgg cgctggcaag
     4021 agcgttgatt catcgaccgg gattattgtt gcttgatgaa ccgctcgggg cgctggacgc
     4081 cttaacgcga ctcgagatgc aggatttgat tgtgtcactt tggcaggagc acggctttac
     4141 cgtactgttg gtgacgcatg atgtgagcga agcggtagcg atggctgacc gggtgctgtt
     4201 aattgaagag ggaaaaattg gtctggattt gacagtggat atcccccgtc cgcgtcgatt
     4261 agggtcggtg cgattagcgg agctggaagc ggaggtgttg caacgggtga tgcaacgtgg
     4321 tgaatctgaa actcgcctga gaaaacaggg gtaaattccc cgaatggcgg cgctattgcc
     4381 gccattcggt tatttatcaa gccagtgctt tagttatctt ctcgtacaga tcgccagaga
     4441 gattttccag ccctttcaac tgttccagcg ccgcgcgcat tttctcctga cgtttggcat
     4501 cgtaacgttt caggcgaatc agcggttcaa tcagacgtga agccacctgc gggttacggc
     4561 tgttgaggtc ggtaagcatt tccaccagga acaggtaacc gctgccatct tcggcatgga
     4621 acgctgccgg attgctgccc gcaaacgcgc caatcaacga acgaatacgg ttcgggttgc
     4681 tcatggtaaa tgagcgatgc tgcaacaggc cgcgcaccgt ctccagcaca ttcgccgccg
     4741 ggctggtggc ttgcaggata aaccatttat ccatcaccag accgttctga tgccacttgt
     4801 cgtcgtactc ctgcatcagc gcgtcacggc aaggcagctg tgcggcaacc gccgcagaaa
     4861 gcgccgccag cgcatcagtc atattgtttg cttcgtggaa ctgcttgctc accagcacat
     4921 cagccagatg cgtttcacca aaagcgagga aacgcaggca ggcattacgc agagtgcgtt
     4981 ttgcaatatc ttcatgctca acacggtact cgctctggta attcgcgttg taaatagcca
     5041 gtagctcatc cgccagttca gtcgccagag tacgagtgag tgcttcgcgt acttcggcaa
     5101 tagcaatcgg gtcgatgata tcgaacaatt cagccatttc attgacagaa ggcagcgtca
     5161 ggatttctgc cgccagcgct ggatcaatct tctcatcaag cagtaccgcg cggaaagcat
     5221 cagccacatg caccggcaga gacagcggct gaccttgctg atgacgcgcg acgttcagct
     5281 tgatgtaggt tgccagcaaa ctttgcgccg catcccagcg ggagaaatca ttacgcgcat
     5341 gacgcatcag gaaggtcagt tgctgatcgc tccacttata ttccagtttc actggcgcag
     5401 agaattcgca cagcagcgca ggcaccggct ggaagtagac attatcaaag acaaaggtct
     5461 gttccgcctg agtgacgttc agcacggaat tcaccggatg accgcctttc tgcaacggga
     5521 tcactttgcc ttcgttatca tacagttcga tggcaaacgg aatatgcagc ggctgttttt
     5581 ctgcctgatc cggcgtggct ggcgtgcgct ggctgatggt cagggtgtac tgctcggttt
     5641 ccggattgta gtcgtctttg acggtcacaa tcggtgtacc ggactggctg taccaacggc
     5701 ggaaatggga gagatcgaca ttcgacgcat cttccatcgc ctgcacaaag tcgtcacagg
     5761 tcgctgcact accatcatga cgctcgaaat aaagctgcat ccctttctgg aagttttctt
     5821 cgccaagcag ggtgtggatc atgcgaatca cttccgcgcc cttctcgtaa acggtcaggg
     5881 tgtagaagtt gttcatctca atgaccatat ccgggcggat cgggtgcgcc atcgggctgg
     5941 cgtcttctgc aaactgcaat ccgcgcatgg tgcgtacatt attgatgcgg ttaactgcgc
     6001 gggaaccaag gtcagagctg aactcctgat cgcggaagac ggttaaacct tctttcaggc
     6061 tgagctggaa ccagtcgcga caggtcactc ggttaccggt ccagttatgg aaatattcat
     6121 ggccgataac gcgttcaata tcgaggtaat ctttgtcggt ggcggtgtcg gtgcgggcca
     6181 gcacatattt ggagttaaag atattcagcc ccttattctc cattgcgccc atattgaaga
     6241 aatccaccgc gacgatcata tagatgtcga ggtcatactc caggccaaag cgttcttcat
     6301 cccatttcat ggagtttttc agcgaggtca tcgcccacgg cgcgcgatca aggttgccgc
     6361 gatcgacgta cagctccagt gctacttcgc gaccagaacg cgtggtaaag gtatcgcgca
     6421 gtacatcaaa gtcgcctgcc accagcgcaa acaggtagca cggtttcggg aacgggtcct
     6481 gccactgtac ccaatggcgt ccgttttcca gttcgccttg cgcaacgcgg ttaccattgg
     6541 aaagcaggaa gggatatttg attttatcgg caataatttt ggtggtaaaa cgcgccagca
     6601 cgtccgggcg gtcgagataa tacgtaatat ggcggaaacc ttcggcttca cactgggtgc
     6661 aaagcgcatc gcctgactga taaagccctt ccagcgcggt attcgccgcc gggcttattt
     6721 cattaatgat cttaagcgta aaacgctccg gcaaattact gataaccagt gcgccctctt
     6781 cttctttcca ggcggtccac ggctcatcat taatatgaac agaaaccagt ttgaggtctt
     6841 cgccgttgag acgaagggga gcatctgatg caccatgacg gacagcctgg ctgaccgcgg
     6901 taacgaccgt cttttgcgcg tcgaggtcaa aggtcaagtc aatatcagta atctggtaat
     6961 ccggcgcacg ataatcgtga cggtatttgg cttgtggctg ttgagtcata aataaccttt
     7021 agcatctttt atagagtctg gtgttcagtc tattcctgtt gcgtaaatcg cgctatgcag
     7081 aatcttcatc ttttcaggta caaacgcctt tattgctaca tttttataac atacaccgcg
     7141 taatgccatc gaccagaaag gtggcatatg gtgtgatcgg ggttcaataa attgcgaaac
     7201 aaggtatact ccagcagttc ctgaagatgt ttattgtact aaacgctcct gtacgaggac
     7261 gctactgcgc acctatgaca caattcgctt ctcctgttct gcactcgttg ctggatacag
     7321 atgcttataa gttgcatatg cagcaagccg tgtttcatca ctattacgat gtgcatgtcg
     7381 cggcggagtt tcgttgccga ggtgacgatc tgctgggtat ttatgccgat gctattcgtg
     7441 aacaggttca ggcgatgcag cacctgcgcc tgcaggatga tgaatatcag tggctttctg
     7501 ccctgccttt ctttaaggcc gactatctta actggttacg cgagttccgc tttaacccgg
     7561 aacaagtcac cgtgtccaac gataatggca agctggatat tcgtttaagc ggcccgtggc
     7621 gtgaagtcat cctctgggaa gttcctttgc tggcggttat cagtgaaatg gtacatcgct
     7681 atcgctcacc gcaggccgac gttgcgcaag ccctcgacac gctggaaagc aaattagtcg
     7741 acttctcggc gttaaccgcc ggtcttgata tgtcgcgctt ccatctgatg gattttggca
     7801 cccgtcgccg tttttctcgc gaagtacaag aaaccatcgt taagcgtctg caacaggaat
     7861 cctggtttgt gggcaccagc aactacgatc tggcgcgtcg gctttccctc acgccgatgg
     7921 gaacacaggc acacgaatgg ttccaggcac atcagcaaat cagcccggat ctagccaaca
     7981 gccagcgagc tgcacttgct gcctggctgg aagagtatcc cgaccaactt ggcattgcat
     8041 taaccgactg catcactatg gatgctttcc tgcgtgattt cggtgtcgag ttcgccagtc
     8101 gttatcaggg cctgcgtcat gactctggcg acccggttga atggggtgaa aaagccattg
     8161 cacattatga aaagctggga attgatccac agagtaaaac gctggttttc tctgacaatc
     8221 tggatttacg caaagcggtt gagctatacc gccacttctc ttcccgcgtg caattaagtt
     8281 ttggtattgg gactcgcctg acctgcgata tcccccaggt aaaacccctg aatattgtca
     8341 ttaagttggt agagtgtaac ggtaaaccgg tggcgaaact ttctgacagc cctggcaaaa
     8401 ctatctgcca tgataaagcg tttgttcggg cgctgcgcaa agcgttcgac cttccgcata
     8461 ttaaaaaagc cagttaatat catcagggag ctaatcggct cccttttttt acctttaatt
     8521 ccgaaatctt tcgctgcatt tgcgaattct gcttgtctga ttgcagatgc caggtaacat
     8581 aggtatcccc ccatttacgg gtggacaagt gtttattttt tccgactatt aacagagaga
     8641 atattatgag cgttgtgcct gtagccgacg tactccaggg ccgtgtagcc gttgacagcg
     8701 aagtcaccgt gcgcggatgg gtacgtaccc gccgagattc aaaagctggc atctccttcc
     8761 tcgccgttta tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat aattctctgc
     8821 ccaattacaa tgaagacgtc ctgcgtctga ccaccggctg ctcggtcatt gtgacgggta
     8881 aagtcgtggc gtcgccgggc caggggcaac aatttgaaat tcaggccagc aaggttgaag
     8941 ttgctggttg ggttgaagat ccagacactt acccgatggc ggcaaaacgc cacagcattg
     9001 agtatctgcg tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt gccgtcgcgc
     9061 gcgttcgcca tacgctggcg caggcgctgc atcgcttctt taacgagcag ggattcttct
     9121 gggtttcaac gccactgatt accgcatctg ataccgaagg tgcaggcgaa atgttccgcg
     9181 tttctacgct ggatctggaa aacctgccgc gtaacgatca gggcaaagtg gatttcgaca
     9241 aagacttctt tggtaaagag tctttcctga ccgtatctgg ccagttgaac ggcgaaacct
     9301 acgcttgcgc attgtccaaa atttatacct tcggcccgac tttccgtgct gaaaactcca
     9361 acaccagccg tcacctggcg gaattctgga tgctggagcc ggaagtggcg tttgctaacc
     9421 tgaacgatat tgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa gcggttctcg
     9481 aagaacgcgc tgacgacatg aaattcttcg ctgaacgcgt agataaagat gccgtttcac
     9541 gtctggaacg cttcattgaa gccgattttg cgcaggtgga ttataccgac gcagtgacca
     9601 ttctcgaaaa ctgcggcagg aagtttgaaa acccggttta ctggggagtc gatctctctt
     9661 ctgagcatga gcgttatctg gcggaagaac actttaaagc accggtagtg gttaaaaact
     9721 atccgaaaga tattaaagcg ttctatatgc gccttaacga agacggtaaa accgttgcgg
     9781 ctatggacgt tctggctccg ggcatcggtg agatcattgg tggctcccag cgtgaagaac
     9841 gtctggacgt gctggacgag cgtatgctgg aaatgggcct gaataaagaa gattactggt
     9901 ggtatcgcga tctgcgtcgc tacggtactg ttccgcattc aggtttcggt cttggttttg
     9961 aacgtctgat tgcttacgta actggcgtgc aaaacgtacg tgatgtgatt ccgttcccac
    10021 gtactccgcg taacgccagc ttctaattta gcgtcttcaa gagccagcgc ccgctggctc
    10081 tttttttatc ttttctgtca agttatctgt ttgttaagtc aagcaatcta tttgcaaccc
    10141 cgccataacg atcatcctgt tacggaatat tacattgcaa catttacgcg caaaaactaa
    10201 tccgcattct tattgcggat tagttttttc ttagctaata gcacaatttt catactattt
    10261 tttggcattc tggatgtctg aaagaagatt ttgtgccagg tcgataaagt ttc
//
LOCUS       DAZFZC010000142        10274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFZC010000142 DAZFZC010000000
VERSION     DAZFZC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.143"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..360)
                     /locus_tag="ACHKXP_004480"
     CDS             complement(<1..360)
                     /locus_tag="ACHKXP_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7649345.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIA"
     gene            complement(627..1292)
                     /locus_tag="ACHKXP_004481"
     CDS             complement(627..1292)
                     /locus_tag="ACHKXP_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO7649346.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1433..2074)
                     /locus_tag="ACHKXP_004482"
     CDS             complement(1433..2074)
                     /locus_tag="ACHKXP_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO7649347.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2783..3814
                     /gene="repA"
                     /locus_tag="ACHKXP_004483"
     CDS             2783..3814
                     /gene="repA"
                     /locus_tag="ACHKXP_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO7649348.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            4510..4677
                     /locus_tag="ACHKXP_004484"
     CDS             4510..4677
                     /locus_tag="ACHKXP_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649349.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLI
                     CRWRGIRITEE"
     gene            4764..5015
                     /locus_tag="ACHKXP_004485"
     CDS             4764..5015
                     /locus_tag="ACHKXP_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7649350.1"
                     /translation="MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYC
                     VPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            5012..5299
                     /locus_tag="ACHKXP_004486"
     CDS             5012..5299
                     /locus_tag="ACHKXP_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7649351.1"
                     /translation="MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSHVYNLASERMR"
     gene            5309..5629
                     /locus_tag="ACHKXP_004487"
     CDS             5309..5629
                     /locus_tag="ACHKXP_004487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649352.1"
                     /translation="MLLSFSELDVLIYQGKVGGLFRERKNERVATDIDSRNKSSSSSH
                     GYDSVLSFQFICDNGKEINVNLTNWNINIFPGQELIIYFVENSKKNKKNTIHDKKQKH
                     IGSD"
     gene            complement(6507..7109)
                     /locus_tag="ACHKXP_004488"
     CDS             complement(6507..7109)
                     /locus_tag="ACHKXP_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7649353.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            7463..8809
                     /gene="yagA"
                     /locus_tag="ACHKXP_004489"
     CDS             7463..8809
                     /gene="yagA"
                     /locus_tag="ACHKXP_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFO7649354.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            9088..>10274
                     /locus_tag="ACHKXP_004490"
     CDS             9088..>10274
                     /locus_tag="ACHKXP_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO7649355.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSVQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNS"
BASE COUNT         2836 a         2302 c         2586 g         2550 t
ORIGIN      
        1 ggcaatcagt ctgccggtgc ggtgtacgag aggacatgtc agcagcaggc aaagtggtgc
       61 aaggagaatg ttcggggcag gagcctgcag cagatgaata ataagaccct gcaggagaat
      121 gccggatata ccaaaaaaac ctgccagcat acagaggacg atcagcaggg ggatccgtcc
      181 gatgttcagc cagtccagcg cctctcctgt cagcccctcc ggtaaatcag gtgtatccat
      241 agctgaagag agggagtgtc cgcagagcag ggcgataagt tcagcgatac cagtaagcag
      301 aacaaaagtc agggctgcca gatagggtac gttatacgcg gtaagtaacg tcagtgtcat
      361 ttttcctcct gtggcaggtc agtcaaacaa cattatggcg tgaagcttcc gcttctttaa
      421 ttgtcagaag aagcatatcg tcagtcatca gaagacaagg ggatgtctgc aggaggggat
      481 gataacaact gaagcctcac gccggaatgc gctttatgac aaccggagag gacggataat
      541 aaaaaatggg acatccacat cagcaaaccg cgatttgcga aaaaaagtca gcggagagtg
      601 gctgccgttg tggctgtccc ggtatttcag tgtaacaggt gattgctgtg atctatgccc
      661 cattcggctt tcggtaaacc tgaatgcccc acatgaagaa tgcgtgacag catgtttctg
      721 ccgctgccgt tcaggctgca ttcgcgtccg tgcgcgagta taaaaccttc atcccggaat
      781 atctcttcca cttctgaaga ttcgagggat gccggatgaa ttaatgaaaa tgccaccggt
      841 ctgagcattc tgcccgtggt ggaaaaggtg tctctggtac tgttcagctg caggatatga
      901 cgtttacctg tttgcgcacg ggaaagtgtc tcactcagca gctggtcgtt ccatgcgcac
      961 gtgccaatct gccatcccag acttctgcac gcggaaagag ccgagcggaa gcggtacagg
     1021 tcgtataatg ctgcgttatt gccactttcc agcagtgatt tcagcgtatt tgctaccggt
     1081 atgtccgtat tggtggccag cagttttcgc agagtcgtaa actcttctgc cattcctctg
     1141 aatgcgctca tctgacgcgc agattcaagc cagttcagca ggtaacgccg gcgacgccgt
     1201 tcgttgttga tactgtccgg ggcggtggag agtgcttcga gcatattctc cagtaacaga
     1261 atgacagggt tttccaggga tagaatggtc ataaaggcgt cctgaaaaaa tgcccgacag
     1321 ccgctctccg tacaccgtaa ggtgtcaccg gtgccgggca ttgtggaaac agaaggaaaa
     1381 aaaatcccgg ccataaaggc cgggaaaagt cgtcaaatta agacagcaat tttcatgtca
     1441 acgctccgtc atcagatacc aggtgcagga gcagggcgat cctcgactcc ggcagaggcg
     1501 gtgcatcaag ccggaggagg tactggtaac gcgcttttgt cagccagagg gtggaggcag
     1561 catccagctc ctccctggct gccggattga gcgcaggatc cgggtacact ttcatcaaag
     1621 actgaacgat atcctccctg acggttttga tttcaccggc cattttcacg aagttcagaa
     1681 aggcactgtg acggcgaagg tgagtgaagc tgtggcgttc ggggtctgct ttcaggaaaa
     1741 agtaaccggg aaacacggcg tgtatatgtc gacggtaacc gcagacttta tccgtgcggc
     1801 ggactttttt cagtatcagg ggtgtccagg gaaggacgtg ttgttcggaa agccaggaaa
     1861 agagatgttc gcggtttttg ccggcaggga tgtactgagc cagataccag ttgcgttgat
     1921 gtaattctct gatattcaaa atccggaacg ctccgcgaaa atcgatcccc gcagaggggc
     1981 ttcgatgttg ttgtgaacgg cgaatacata aagcatgtgt cggaaacagc ggctgatcct
     2041 cgttcagccc ggcctgcagt tctgacaggc acatgattta cgcactcttg taagccttgt
     2101 ggacggtttc gaggccggcg aaaaccgaca aaacctgcaa acaggattaa tggctggcaa
     2161 acatattaca taaggatata tctgtggcaa gagcgaagat aagtagttaa atagatcgtt
     2221 atatttgata aagcagcata ttacgctggg ttgtatgagg tgtttggatg attagatcgt
     2281 agatcgtaga tcgtaattac aagatcgatt catccaaaaa acatgaaaaa cacagcataa
     2341 aagatcgttt gtggaattgt ctattttttg acacagttca aaaaaacgac gttttttagg
     2401 tggttaatga gaggcctggt aaatcagaga aatcgtgttg gcttttcaaa gcggtggaaa
     2461 aggggtatat tgcggatcgt tatgcagtgg cttttgggat cgtcaggatc cgggaagtca
     2521 gaaaatggca ggatgcgcca taaggcattc aggacgtatg gcagaaacga cggcagtttg
     2581 ccggtgccgg aaggctgaaa aaagtttcag aaggccataa aaggaaaacc cccataatct
     2641 tctcatcgaa cttggcggaa cgttagaaga ttatgggggc ctcacagaat acgggataag
     2701 tatatatgaa accgtgtcag agattcaacc cttcgcagtg tattaacgca cggcattctg
     2761 tttccgccat aagcgacagc ttgtggcagg tctgaagaat actccatata acgcagtaca
     2821 ctggagtcag ttagcacccg aagagcagat ccgtttctgg gaagactatg aagcgggaag
     2881 ggcgacctct ttcctggttg aaccggaaag gaagcgcacc aggcgccgtc gtggcgaaca
     2941 ctccactaaa cccaaatgcg aaaatccgtc ctggtatcgt cctgcgcgct ataaggagct
     3001 gagcgggcag ctcgggcacg cctacaaccg tctggtgaaa aaggccccgg tgaccggcga
     3061 gcagagcctg cgtatgcgta tgtcgcggca tcctttttac gtgcagaaac ggacgttcgt
     3121 cggtcgcaaa tatgctttcc ggccggaaaa acaacgcctc ctcgatgcca tctggccggt
     3181 tctggtcagc ttcagtgatg ccggcacaca cactgtgggg atgagtgtca ctcgcctggc
     3241 taaagaaatc agtccgaaag acagtaaggg aaatgttatt ccggaactgg aagtgacggt
     3301 ctcccgtctt tcccgtctgc tggccgaaca ggtgcgtttt ggtgtgctgg gtgtttcaga
     3361 ggaaacgatg tgggaccgtg aaacccgcca gcgtctgccg cgctatgtct ggataacacc
     3421 ggctggctgg cagatgctgg gcgtcgacat ggtaaaactt cacgaacagc agcagaaacg
     3481 actgcgtgaa agtgaaatcc gccagcagct cattcgggaa ggtgttctgc gtgaggatga
     3541 agatatctcc gtacatgcgg ccagaaaacg ctggtatctg cagcgcagtc aggatgcgct
     3601 gaaacaccgt cgtgcgaaag cagcagccag taagcgcgcc agacgcctga agaaactgcc
     3661 tgccgaccag cagattcatg agatggcaga gtatctcagg aagcgtctgc cgccggatga
     3721 agcctatttt tgttccgatg accatctgaa gcgactggcc atcagggagt tgcgtcagct
     3781 tgaactgacg ctggctgccc cgccaccgca ctagacagca ccattccctc agcactgaat
     3841 catcgccagc ccctccgggg ctttcggcgc aggtatcgct cagcccaaaa tccgcagtaa
     3901 tcaccttaaa tcccctcaga ggggcatatt tgctcataaa accacgcatc agtcatcaga
     3961 acgtggccac gttgtttcag ttatccacat aaatccgcaa acaaagaact ttaagaagct
     4021 gcaaacctga aacagcaaac ctgcaatata gtcttaacac cattatttaa tcccctgcgt
     4081 tgcttcgccg cagggaaaat ctttatctct gaaaccactg tgaacaaata caaagaggcc
     4141 ttcgcttgca gcggccaagg ccgcgccgct cagaatctga aagtacctcc cacgctgacg
     4201 cgcgggcccc gaacctcacc gttctgaaac cacaacaaaa aaacatcagg aataaaaaca
     4261 ccacacaaac gcagcaccgt gcccacccct cataactgaa aagcgaggcc gcccccgccc
     4321 aaagggcggg aacaacatcg cttttaatta tgaatgttgt aactacatta tcatcgctgt
     4381 cagtcttctg gctggatgtt ctcagtacac gctcgtaagc ggccctgacg gcccgctaac
     4441 gcggagatac gccccgactt cgggtaaacc ctcgtcggga ccactccgac cgcgcacaga
     4501 agctctctca tggctgaaag cgggtatggc ttagcagggc tggggatggg taaggtgaaa
     4561 tctatcaatc agtaccggct gacgccgggc ttcggcggtt ttactccggt atcatatgaa
     4621 acaactgagt gccgccttat atgccgctgg cgcggcatca ggataactga agagtgacag
     4681 tacacattaa catcaaacag acttgttaag cagtctttca gggtgtaagg tgaagacctg
     4741 ttaaacagtc tcagaggtgt gttatgccca gtatcatttt gtctgacacc agtgcaagcg
     4801 tcagtgaact gaaaaaaaat ccaatggcga cagtcagtgc cggagagggg tatcctgttg
     4861 ccattcttaa ccggaatcag cctgcattct actgtgttcc tgctgaattg tatgaaagga
     4921 tgcttgatgc tatggatgac caggagctgg tgaaactggt tactgaacgc agcaatcagc
     4981 cactacatga tgtggacctg gatagttatt tatgacttat acggttaaat tcagggatga
     5041 tgccctcagg gaatggctga aactggataa atccgttcaa caacagttcg caaaaaaact
     5101 aaaaaaatgc agcgaaaatc ctcatatacc ttcagcaaaa ctacgtggat taaaagattg
     5161 ctacaaaata aaacttcgcg catcaggttt tcgtctggtc tatcaggtga ttgatgacat
     5221 gttaattatt gcggttgtcg cagtaggtaa acgtgaacgt agtcatgttt acaatctcgc
     5281 cagcgagaga atgagatagg gggtgtaaat gcttttaagt tttagtgagt tagatgttct
     5341 tatttatcag gggaaggttg gggggttatt tcgtgaaaga aaaaacgaaa gggttgctac
     5401 agacattgat tccagaaaca agtcatcatc aagcagtcat ggatatgatt cagttttatc
     5461 atttcaattt atttgtgata atggcaaaga aataaatgtg aatttaacaa attggaatat
     5521 taacatattt ccaggtcaag agcttattat ttattttgtg gaaaactcaa agaaaaataa
     5581 aaaaaatacc atacatgata aaaaacaaaa gcacatcgga agtgactaat aatgttcaag
     5641 aaatgtataa agagtttttt tatacatcat acttcaaact gataatacct ttcctgttaa
     5701 tcatttctta ctttatgtca tccatatctg tggctatcat gtcattcgtt ttatatgtca
     5761 taatcagaag aagaatagga aaagatttct tttcagaaat aaaaaagaaa aggttaaaaa
     5821 aagaagatat catggggtta gagcacgtaa tataggggcg actggtcctt ataagggacc
     5881 gtctagttat cgcggtaggg tgtactcccg cgctatacgg tgttttcaga ccgcccgttg
     5941 aagcaggaac cagacgctga gcaatcagcc gtcacccact aatgggaatc cccctcctga
     6001 gtcacgaagt gcgaaggtgg gggaggatgt caagtgtgca gtatcaaaaa actgtatcac
     6061 tggtataatc cagtaaagag catattgaga acttattatg tcgtcacgca actctgtaca
     6121 tgattttata atgctgctcc cggtgctact gttggtcggg tgggtagttt ttgtgataat
     6181 tctcatgttt cgggatgata aatgtgacaa aacttaccgt gaaggttcgt ttcccgaatt
     6241 tatttgtctc cattctgatc gctcgtcact tcgagaaacc gattctgaga aactcattgt
     6301 caactataca gaaatacagg tatacaatca ggagaatagg taaaatccat aatactatag
     6361 ctaaaaaaat atatacgggg atcaggcgtc tgtatgcccg ttcatgttta ttcaggatga
     6421 ataaaatctt ctccgcttct gaatcacaca taacattctc ttcccatttt ttctgatacg
     6481 taatgtccac ctgttatcag gcgtgtttat tgcccagaaa tattctcctg ctgtgcctgc
     6541 atcctgagcc acttttgttc taatgtcgcc agtctgcgac cggcactctg tgcttcagag
     6601 tctgagacct gccctttggg gtgaccatcc aggccataac gtgggacgcc ggcgacagct
     6661 gtccgctgca gatatacgcg gcgggatgtg atgcaggaca ctgcacagag ccattcgtcc
     6721 atggtcagtt ctgtctccgg gtgttctgcc agaaatgctg aaatatcatt gtttatgcca
     6781 attttcaggg gaataaatcc accaccttca gcacgaagat gaggaaacag ggcctttatt
     6841 ttttccacgc actcgtgagt atactcacgg gtccagtggg agggacgtgg aaaacggtgt
     6901 cttttctttt tcttcttcct ggctggcgct tcggggatat gtgtttcgac ggcagatgaa
     6961 tttattccgg cgggggctga ttctgccgtc tgttcagtaa atacaggtgc gataatatct
     7021 gttttttcgg ataaggacgg tagcgaaaaa gtacgacgtt ttttcaccac aatgacggga
     7081 gttttccttt cttcctgatt ttttcccata aatacctcgg ctggctggtt ttactcttta
     7141 ataatatcac aattcataaa ttagtaaatt ttcaatcagg tgatttttca tggctaataa
     7201 gtccgttctc tgaatattta tactcgaggt agtaacagtt atggcgtgac agtcgaattg
     7261 atattttact gaaccctgac agaaacatgg ttgatatatt ctctcacttt aatgttgtca
     7321 tgatataaat aaaaacaatt catttgaaag gatatcagaa gaagatatcg gggcgcacag
     7381 ttcatctctg cctttgtttc atggggtgtt ctttgtatat tttacttact gtttaagcat
     7441 gtacataagg agtgctttca ttatgccaat gatacttgta aagaagaatc cccggggcaa
     7501 agtgattcgg gagcttagca gtgaggaaga gactgcagta aagacggtct gtggactgaa
     7561 aaagccagca accatggcac tacataacct ggccaatgat attctccggg aaatgcgtga
     7621 gtatgatgca tggttacagt gtgactgtat tccgggagat tcgccggcca tgaattttgc
     7681 tgctcttaaa aacaatacgg gtactcttta tctgtccagt tttaatcatg agcatgcacc
     7741 tgaatgcccc atgtatcgtc agttatccgg aaatgaagag gaaacatctt ccggagcaag
     7801 ccggcatccc gttagcaccc gtataaatta cagaaacttt ttaccaccag atgacagtaa
     7861 ttctgttatc agactgcagg cccgctctgc ttaccataat ggtgagagct cctccgttcg
     7921 caagaaacgt ccgcgccttg cccgtttact cctgtctctt attgacgatg ctgggctgaa
     7981 taaactggat tccctggcta atccccgtat ccggactaat tatcgtgagt gtcttgatgc
     8041 catacggcag gttacactgc agcaggagta tattcgggga agatccttat ctgaaattat
     8101 ccatttccgg cctggaatgt ctgaaaggtc tcaggagcgg ttaatggaaa cgctggagaa
     8161 ttcagaacga cactggcctg ccagaagaaa acatatgttc tttcagattt ttatggcgca
     8221 gcacatctgt cgtgatgctg tggaaattca ctgggcgaac ggtaatattc aggtcatcag
     8281 acctgtgcgg gggatcagta ttaatggtga agcgcagggc ggaatacgtc ccccttactg
     8341 ggttattctt gctttttgcc ggagtgccga tggcagaatc atctgcagtg aagggtatgc
     8401 tcatgctctt tatcaactga catgtccggt gcctgtggat agcaaactgg agcgaaacac
     8461 gctcactgct cttctgaatg tggccagctg gcttaaaaga aagccaggta cgccggaatt
     8521 aagtctggaa aggcccctgt ttgatacaga agtttatgtt aatggtgaaa agaaatatgt
     8581 cctgccggat ttcattgtca cagcaagggc tcctgacgga aagacggcca gagtggtcat
     8641 cgaaacgatg ggatatgaag acagtgatta ctgcgcgaga aaatccaggc agcataccgg
     8701 catgaagcag attggtgttc tgcataccga tccaccgaaa tggctggata acgatcatcc
     8761 cccttttgag aaacatatgt acggtgtttt tatgcatctc aggtactgag atattttgtg
     8821 gctcagttct gtaacttttc ccgtaacatt gtctgttgtt acgggaaagt ccggtttttg
     8881 tattgcacca gagaatactc agactgtgat gctgccacag cgtcagcagg ctttctgaac
     8941 ggtgtaacat cacttcatgt taatgataat cactatcatt aaatcttgac atgccatttt
     9001 ctccttaata aattgatact gtatatgtat ccatatacgt aagcagttaa ttcatttgtt
     9061 ttcctcagag gatgaaggag ataccgaatg tctgaccctg tacgtattac aaatcccggt
     9121 gcagaatcgc tggggtatga ttcagatggc catgaaatta tggccgttga tatttatgta
     9181 aaccctccac gtgtcgatgt ctttcatggt accccgcctg catggagttc cttcgggaac
     9241 aaaaccatct ggggcggaaa cgagtgggtc gatgattccc caacccgaag tgatatcgaa
     9301 aaaagggaca aggaaatcac agcgtacaaa aacacgctca gcgtgcagca gaaagagaat
     9361 gagaataagc gtactgaagc cggaaaacgc ctttctgcgg caattgctgc aagggaaaaa
     9421 gatgaaaaca cactgaaaac actccgtgcc ggaaacgcag atgccgctga tattacacga
     9481 caggagttca ggctcctgca ggcagagctg agagaatacg gattccgtac tgaaatcgcc
     9541 ggatatgacg ccctccggct gcatacagag agccggatgc tgtttgctga tgctgattct
     9601 cttcgtatat ctccccggga ggccaggtcg ttaatcgaac aggctgaaaa gcggcagaag
     9661 gatgcgcaga acgcagacaa gaaggccgct gatatgcttg ctgaatacga gcgcagaaaa
     9721 ggtattctgg acacgcggtt gtcagagctg gaaaaaaatg gcggggcagc ccttgccgtt
     9781 cttgatgcac aacaggcccg tctgctcggg cagcagacac ggaatgacag ggccatttca
     9841 gaggcccgga ataaactcag ttcagtgacg gaatcgctta acacggcccg taatgcatta
     9901 accagagctg aacaacagct gacgcaacag aaaaacacgc ctgacggcaa aacgatagtt
     9961 tcccctgaaa aattcccggg gcgttcatca acaaatcatt ctattgttgt gagcggtgat
    10021 ccgagatttg ccggtacgat aaaaatcaca accagcgcag tcatcgataa ccgtgcaaac
    10081 ctgaattatc ttctgaccca ttccggtctg gattataaac gcaatattct gaatgaccgg
    10141 aatccggtgg tgacagagga tgtggaaggt gacaagaaaa tttataatgc tgaagttgct
    10201 gaatgggata agttacggca acgattgctt gatgccagaa ataaaatcac ctctgctgaa
    10261 tctgcggtaa attc
//
LOCUS       DAZFZC010000143        10113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFZC010000143 DAZFZC010000000
VERSION     DAZFZC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.144"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..612)
                     /gene="yncG"
                     /locus_tag="ACHKXP_004491"
     CDS             complement(<1..612)
                     /gene="yncG"
                     /locus_tag="ACHKXP_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO7649356.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NIYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEI"
     gene            879..2378
                     /gene="ansP"
                     /locus_tag="ACHKXP_004492"
     CDS             879..2378
                     /gene="ansP"
                     /locus_tag="ACHKXP_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO7649357.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2491..3552)
                     /gene="yncE"
                     /locus_tag="ACHKXP_004493"
     CDS             complement(2491..3552)
                     /gene="yncE"
                     /locus_tag="ACHKXP_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO7649358.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     ISRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3794..5896
                     /gene="pqqU"
                     /locus_tag="ACHKXP_004494"
     CDS             3794..5896
                     /gene="pqqU"
                     /locus_tag="ACHKXP_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO7649359.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTVEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHSYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTVNELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(5932..6597)
                     /gene="mcbR"
                     /locus_tag="ACHKXP_004495"
     CDS             complement(5932..6597)
                     /gene="mcbR"
                     /locus_tag="ACHKXP_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO7649360.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVTPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPPDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6795..7832)
                     /gene="curA"
                     /locus_tag="ACHKXP_004496"
     CDS             complement(6795..7832)
                     /gene="curA"
                     /locus_tag="ACHKXP_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO7649361.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8013..8531
                     /gene="mddA"
                     /locus_tag="ACHKXP_004497"
     CDS             8013..8531
                     /gene="mddA"
                     /locus_tag="ACHKXP_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO7649362.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8528..8977
                     /gene="ydcZ"
                     /locus_tag="ACHKXP_004498"
     CDS             8528..8977
                     /gene="ydcZ"
                     /locus_tag="ACHKXP_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7649363.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8978..9211)
                     /gene="ydcY"
                     /locus_tag="ACHKXP_004499"
     CDS             complement(8978..9211)
                     /gene="ydcY"
                     /locus_tag="ACHKXP_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO7649364.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYAQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9297..9470)
                     /gene="ortT"
                     /locus_tag="ACHKXP_004500"
     CDS             complement(9297..9470)
                     /gene="ortT"
                     /locus_tag="ACHKXP_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO7649365.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9665..9760
                     /gene="yncL"
                     /locus_tag="ACHKXP_004501"
     CDS             9665..9760
                     /gene="yncL"
                     /locus_tag="ACHKXP_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO7649366.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2428 a         2471 c         2480 g         2734 t
ORIGIN      
        1 aatttcattc ctttttaata cttcttgtaa tttcggtaac tgacatacag catcagcgat
       61 cgcgctgata ttctgcgcat tatcctgaaa ccattcatgc ccaggccccc atgtacgcat
      121 agtgcaaaga taacagtcca ccagcgttag ttgctcacca aacgcataag gttcagcagt
      181 gagctgcgaa ttcaaccaga tataaagcga ttttcgatat tcaataacat tcttctttaa
      241 ctgttcaggt gcgtcaggag cccaacgctc agggtaatcg gcgaaagtga atgttggata
      301 gatattagca accagccaga ccaatagccg ttgaaactgc tgacgttcgg cgcgcccaac
      361 cggtggcgca aggtccgggc gacgatcgag aaccatcaat gcaatcgccg ctgtctccgt
      421 cataatctcg tcattttcca gagccagtgt cggaacctga cacaacgggt tcagggtttt
      481 caataactcg cgtgaagttc cctcgtggtc aaaaccactg acatcaacga actgataagg
      541 aatatcagcc agtgttagca tcagctcact gattgtagag ccccatccgg gtacgccata
      601 caccttaatc atatgcccct cacaatcttt taaagattaa gtgtaggaca gtacacaggt
      661 taaccagcaa aaggatttgt cattttttcg tcagttgtat gaatcccctt caggattgta
      721 aggggatgtt ttgcatcgtt tatcgataac gggtaggatg ctgcgccgat agttttatac
      781 ccataaagaa taatggtgat aactatcatc gccaggatga ataaacattg ttcatggcaa
      841 cttatatgac tttttcatta aagcaatcag ggagagcaat gagtaaacac gacaccgaca
      901 cttcagatca acacgccgcg aaacgccgct ggcttaatgc ccacgaagag gggtatcaca
      961 aagcgatggg caatcgccag gtacagatga tcgccattgg cggcgcgatt ggcaccggct
     1021 tgtttttagg tgcaggagcc cgactgcaaa tggcgggtcc agcactggca ctggtttatt
     1081 taatttgtgg cttgttttcg ttttttattc tgcgtgcatt gggtgagctg gtgctacacc
     1141 gcccttccag tggcagtttt gtctcttatg cccgtgagtt tttgggtgag aaagccgctt
     1201 atgttgctgg ctggatgtac ttcatcaact gggcgatgac ggggattgtt gatattaccg
     1261 ccgtcgctct gtatatgcat tactggggtg cgtttggcgg cgtgccgcag tgggtctttg
     1321 cgctcgctgc acttaccatc gttggcacca tgaatatgat cggtgtgaaa tggtttgcgg
     1381 agatggagtt ctggtttgca cttattaaag tgctcgccat tgtgaccttt ttggtcgtgg
     1441 gtacagtgtt cctcggtagt ggtcagccgc tggatggcaa caccactggc tttcatttaa
     1501 tcaccgataa cggcggcttc ttcccccacg gtttgctgcc tgcgctggtg ttgattcagg
     1561 gcgtagtgtt tgcttttgcc tccattgaaa tggtgggtac agctgccgga gaatgtaaag
     1621 atccgcagac catggtgcct aaagccatta acagtgtgat ttggcgtatt ggcctgtttt
     1681 acgtcggctc cgtggtgttg ctggttatgt tattgccgtg gagcgcgtat caggcggggc
     1741 aaagtccgtt cgtgacgttt ttctctaaac tgggtgtgcc gtacatcggc agcattatga
     1801 acattgtggt gctgaccgct gccctctcca gcctgaactc aggtctgtac tgcaccggac
     1861 gtattctgcg ctcgatggcg atgggcggtt ccgcaccgag ttttatggcg aaaatgagtc
     1921 gtcagcatgt gccgtatgcc gggattctgg cgacactggt tgtgtatgtc gtcggcgtat
     1981 tcctcaacta tctggtgccg tcgcgcgtat ttgagattgt gttgaacttc gcgtcgctgg
     2041 gaatcatcgc ttcatgggcg tttatcatcg tgtgccagat gcgcctgcgt aaagcgatta
     2101 aagaaggcaa agcagcggat gtcagtttta aactgcctgg cgcgcccttc acttcctggc
     2161 tgacattact gtttttactg agtgtccttg tgctgatggc gttcgattac ccgaacggga
     2221 cttatactat cgcggcgctg ccgattatcg gtattctgct ggttataggc tggtttggtg
     2281 tgcgcaaacg cgttgctgaa attcacagca ctgcgccagt cgtcgaggaa gatgaagaaa
     2341 aacaggaaat tgtgtttaag cctgaaacgg cgagttaatt gcaaaattgc ccggctacgt
     2401 gccgggcaaa ttcagattac tgacgaaagt gccggatacg ccgtgataaa aggtaggcaa
     2461 gtctctccgt tttcaccctc gccatatcta ttacagcgca atacgaatca catcgtctgg
     2521 ctgggtagct tcctgctgtt tagtggattt ttgtttcaca ctgacataca gcgttttgcc
     2581 atcggcagac agcgccaggc tgtttggatg agtcggcgta tcgaacgttt tcaccacttt
     2641 atagcttttc gcgtcaatca cactgacttt acctgcctga cgatgcgtta cgtaggcttc
     2701 attacgcgct gggttaaaca gcacagccag tgattccggt gccgcaacct tcgccagaat
     2761 attgccatta cgggtatcga ccactaacac ttctgcggct ttagaatcgg tgataaatgc
     2821 acgctgcctg gcggtatcaa ggctaatgtt gataaagaag tgctctttgc cgtcatccag
     2881 cagcttttta cggctgatga ttttattgtc ggcggtgtcg atggtaatca attcgccgtc
     2941 agcgttagtg gtgtaaagac gtttgccttt gctatccagc gccagaccgg tactcatttt
     3001 accggtgttc tggatggcgg tttttagttt gatattctcg ccatcaacga cccaaatcac
     3061 gctctcttta ccaataccac tgatgtaaac ggtgttcgtg gcatcgtctg ccaccagctc
     3121 acgcggttgc agcgggcgca cctcttccgt gcgcttacga tcatccagca ccagacggcc
     3181 tttcacctcg ccagttttgg catctatcgc cgtgaccgcg ctgtttacgg tgttaccaaa
     3241 ccacaacgtc tgagtcgtgt tattgatggt ggcaccaaac ggcttgagat cgttatggat
     3301 cgcctgggtc acttccaaag tgaccggatc aagacgataa accacaccgc ctttatccag
     3361 tttgcggctt tgcgaagtgg cgagccacag cgcgttttct tgctggctat aagccatttc
     3421 gtaggcacct ttacctaccg ctttacgcag catttcttct gcggcctgcg tactgaatga
     3481 tgaagcaaca agcaatgaac ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg
     3541 acgtaaatgc atgacgactc cctttgatga accgatattt ttgctgcttc acaatgcttc
     3601 gctaagcgta atcatcgtta cgctcttgtt ataaatggga atggtaatca ttattttctc
     3661 aaatgtggag catttcttgc cgttacgact acaattttct caattactta acgtatcgtg
     3721 ccgtttcgtt ttccataaat ttacaatttt attaccatga cggcgttttc ccattcttaa
     3781 cctggctttt ttcatgaaga ttttttccgt ccgacagacc gttttgcccg cactgcttgc
     3841 cctttccccc gttgtttttg ccgctgatga acagactatg attgtcagtg ccgcaccgca
     3901 ggtggtttcc gagctggata cgccagcagc agtaagcgtg gtggatggcg aggagatgcg
     3961 cctggcaaca ccgcgcatta acttgtctga atcactgacc ggagtgcctg gtttgcaggt
     4021 acaaaaccgg cagaactatg cgcaagattt acagctgtcg attcgcggat ttggctcccg
     4081 ctccacttac gggattcgcg gtattcgcct gtatgtggac ggtattcccg ccaccatgcc
     4141 cgacgggcaa gggcagacat ccaatatcga tttaagcagt gtgcaaaatg tggaagtgct
     4201 gcgtggcccc ttctctgccc tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac
     4261 ccagaccgga caacagccac caaccgttga agccagtagt tactacggca gttttggcag
     4321 ctggcgctat gggctgaaag caaccggcgc aacgggcgac ggcacacagc ccggcgatgt
     4381 cgattacacc gtctcaacca cgcgttttac gacccacagc tatcgtgacc atagtggcgc
     4441 acagaaaaat ttagccaatg ccaaactggg cgtacgcatt gatgacgcca gcaaattaag
     4501 cctgattttc aatagtgtgg atatcaaagc agatgaccca ggtgggctaa ccaaagcaga
     4561 atggaaggcg aatccacaac aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat
     4621 caagcaaact caggctgggt tgcgctatga gcgtagcctg agcgcgcaag atgatatgag
     4681 tgtgatgatg tatgccggag agcgagaaac gacccagtac cagtcaatcc cgatggcccc
     4741 ccaacttaac ccgtcgcatg cgggcggcgt gattactctg caacgccatt atcaggggat
     4801 agacagccgc tggacacacc gtggtgaact gggcgttccg gtcacgttca ctaccggcct
     4861 gaactacgaa aacatgagtg aaaaccgcaa gggctacaat aactttcgcc tgaatagcgg
     4921 cgtgccggaa tacgggcaaa aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa
     4981 cgtcgatccc tatttacaga cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg
     5041 cgtgcgctac agctccgtat ggtttgattc caacgaccat tacgttactc cgggtaacgg
     5101 cgatgacagc ggtgatgcca gttatcacaa atggctacct gccggatcgc taaaatatgc
     5161 aatgaccgat gcctggaata tctatctggc agccgggcgt ggttttgaaa cgccgacggt
     5221 taatgagctg tcttatcgtg ctgatgggca aagcggtatg aactttggtt taaaaccatc
     5281 caccaacgat acaattgaga tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct
     5341 cgcattgttc cagaccgaca ccgatgatga aattgttgtc gatagcagta gcggtgggcg
     5401 tacgacttac aaaaatgccg gaaagacccg tcgtcaaggc gctgaactgg catgggatca
     5461 acgtttcgcg ggagattttc gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg
     5521 cagcaatgtt tgcaacgaac aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa
     5581 tatgggcttt gcgtcgatag gttatgtacc ggaagatggt tggtatgcag gcacggaagc
     5641 gcgttatatg ggcgatatta tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac
     5701 tctcgtcggc ttattcaccg ggtataaata caattaccac aatttaactg tggatttatt
     5761 tggtcgtgtc gataatttat tcgataaaga atacgttggt tctgtcattg tcaatgagtc
     5821 aaacgggcga tattacgaac ctgcgcccgg acgaaattat ggtgtcggca tgaatattgc
     5881 gtggagattt gagtaattac cttgatgccc ggtatttgcc gggcatttaa tttaacgatt
     5941 gtattgctgg tataaaatag caatattttg ttgcataatt tcagcaagac aatgtttgca
     6001 tccctctttg tcttttgctt ttaacgcggc gagtaataga cgatagtttt ctatacgctc
     6061 ccgtaattcc gctggattaa tcgcttcata gagataatgt aaaccaggtc ccatcctgac
     6121 ccacagttgc tcaatcatct cacacaggat gggcatattt gagcgatgat aaatcgctaa
     6181 gcgaaatagc ctgttcgcat taatgatttg ttccatgtcg cccttttctt gcgcctgctg
     6241 taatttctca agcaattcct cgagttccgc caggtctggc ggggtgaggt tttcaacagc
     6301 cagagtaact gccattaatt ccagttcgta gcggatccga ttgatttcgt ctaattgacg
     6361 tttccccact tccggaactg taaatgcttg cgctggtgtg acagaaagtg cattcaccga
     6421 aaccagacgt aataatgctt cacgcacagg tgtaatactc atacctaatt gctccgccag
     6481 atttttagta atcaggcgtg cgccaggttt taatgcgcca atactaagct gatgtttcag
     6541 gtcgttctca acctgcagag tcaaactgac atgtttcatt tttcccgttc caggcatttt
     6601 aattccttcg cgtgttttct gtccaaccct gcctgcatgg cagaaggagt tcacctggtg
     6661 tattaaagtg aataatcata aaccactcac ctttgttgtt attagcagca gaacaatgac
     6721 gtggttagtt aattaaagct gaaaataagc tttttgcctg gatttttatg gcggcttacg
     6781 ccgccgttga aactttaatc atcacccgcc acgcggatca ccactttgcc gaagttttta
     6841 cccttcagca ggccgataaa cgtctgtggc gcattctcta aaccgtcagt aatttcttcg
     6901 cggtagtgga ttttatcctc tttcacccat tgccccatct ccttctgaaa ctcatggata
     6961 cggtgaccat aatcctgagc gataataaat ccttgcaagc gaatacgttt tttcagcacc
     7021 gtagccatca gcagaggtaa acgatccgga ccgggtggta gctctgtagc gttatagctg
     7081 ctcactaagc cacaaacggg aattcgcgca gatgtattaa gtaacggtag caccgcatca
     7141 aataccttac cgcccacgtt ttcataatag atatcaatac cttttgggca cgctttcgcc
     7201 agttgttcgg caaaatcgtc cgcgtggtga tcaagacaaa catcgaagcc taacacctcg
     7261 gtagcatggc ggcatttttc cgcgccaccg gctaccccca ccactctgca acctttaagt
     7321 ttgccgattt gccccaccgt cgcccccact ggtcctgtcg ccgcagctac caccaacgtt
     7381 tcgccctctt taggctgacc gatatccagc agccccatat aagcggtaaa gcctggcatc
     7441 cccagcacac ccagcgacca cgatggattt tgcggatgat cgccaagttt caccagatca
     7501 tcaccactgg atatgtcata gtcctgccat ccactgtagc ccagcaccca gtcgccaggc
     7561 tgataatcag gatgattcga ctccacgaca cggctcaccg taccgccgac catcacgccg
     7621 ccaatatcaa ccggcggtga ataagatggc tcatcgctca tacgtccacg catatacggg
     7681 tccagggaca aataaactgt gcgcagtaac acctgtcctt cacccggtgt ggcgacatca
     7741 tcttcttcaa gacggaaatt ctccggaaca ggtgcgccat gtggacgcga ggccagaacc
     7801 caacgtcgat tacgctgctt ttgttgcccc ataatgctct cctgtgcttt tggcatgaat
     7861 ccatcaatag tagcaggctc acgggattgg ttttgtttgc agccgtcaca tcccctctgc
     7921 aacacgcaat aatttcatcg aggaatccgt tgcttcgggc tgttttcgat agtattgata
     7981 acatcatcat cattatttta ttgaggccag acatgtccat ccgttttgcc cgcaaagccg
     8041 actgtgctgc cattgcggaa atttataacc acgccgtgtt gtatacggcg gctatctgga
     8101 atgaccaaac ggtggatgca gataaccgca ttgcctggtt tgaagcgcgg accatagcag
     8161 gttatccggt gctggtaagt gaagaagatg gcgtagtgac gggatatgcc tcgtttggcg
     8221 actggcgtag tttcgatggt tttcgccata ccgtggaaca ttcggtttat gtccatcccg
     8281 atcatcaggg caaaggtctg gggcgtaaat tgttaagccg attgattgat gaagcacggg
     8341 attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca aaatcaggcc tcgctgcatc
     8401 tgcaccagtc gctgggattt gtcgtcaccg cacaaatgcc gcaggtgggc actaaatttg
     8461 ggcgctggct ggatctgaca tttatgcagt tgcaactcga cgaacgcact gaaccggacg
     8521 caattggatg aatcagtcgc tcacccttgc ctttttgatt gccgccggaa ttggtctggt
     8581 agtccagaac acgctgatgg tgcgcatcac ccagacctcc tctaccattc tcatcgccat
     8641 gttgctgaac tcactggtgg ggattgtgct ctttgtttct attttatggt ttaaacaagg
     8701 tatggcgggg tttggcgaac tggtgtccag cgtgcgctgg tggacactta tacctggctt
     8761 gctgggatcg ttttttgtct tcgccagtat cagcggatat cagaatgtgg gagcggcgac
     8821 aactattgca gtactggtcg ccagccaact gattggcggt ctggttctgg atatcttccg
     8881 cagtcatggc gtaccactac gggcgttgtt cggcccaatt tgtggcgcaa ttttacttgt
     8941 ggttggcgcc tggttggtag ccagacgctc attttaatca caggatggtg ccgcctttgg
     9001 ttaactgttc atggcgcgct tccatatctt ctttatgctt gcgaccgtga tgagcaatag
     9061 cggtgcgcaa acgctgctgc tgggcgtagc gatcttcccg acttaactcc gcgtcatcgc
     9121 tcagggcgat taataattcg ttcatatggg caatgacgct ctcttcaatc gcggcatcca
     9181 cgcgcgcgat gacttcatcc agatgcgaca tactttctcc ttagccactt gtgaaattta
     9241 tcgattcatc caaatgatat aagtctgcgt ggtgccgtca agccagtgag taagatttaa
     9301 aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc atgttgcccc caccagaaag
     9361 gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa accaggtgat aagaaatgcg
     9421 attgctgcca taaccgcata aaaaacaagc atgtgttgat agagagacat gtacgggcgt
     9481 ccagccagat gaataaaacg cgcgcaaata tgcgtcattt tttgacgtgc atcaagtttt
     9541 ttaaccggtt tgagaaaaaa acatcaaaca gcaacttgcc cttatccatc ttcagaatga
     9601 gtattattgc ggattttctt aacccgtact atacacatag gggcaatgat aaaaggtggc
     9661 aaaaatgaat gtttccagta gaactgtagt actgataaat gtctttgctg ctgttggttt
     9721 gtttactctt atctctatga gatttggctg gtttatttga tgtagtgatt ttgcacgcag
     9781 cacgtactgt cagagaaatc agtgctctga cagtacgcaa gatcttctca tactctccag
     9841 gtttacaaca tcatccttaa tatttacttc tttttgctgg catctacatt gaaatgagta
     9901 gaaaacatcc ccctactgtc gtctatacaa acagtttact gattcaaacg gaatcttgtt
     9961 ttcacatttt tctattatga atactttata cgcgcgggag tctataaatg ctttatactt
    10021 tcaaggaata acgtaaatat aaaaagatgg cgtaaagtat atgcatggca ggatgtttgc
    10081 aacctccttg atgacgctgc cccattgaaa ttg
//
LOCUS       DAZFZC010000144        10111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFZC010000144 DAZFZC010000000
VERSION     DAZFZC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.145"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..81
                     /locus_tag="ACHKXP_004502"
     tRNA            6..81
                     /locus_tag="ACHKXP_004502"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHKXP_004503"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHKXP_004503"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHKXP_004504"
     CDS             complement(467..1225)
                     /gene="yhdZ"
                     /locus_tag="ACHKXP_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO7649367.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLAVHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHKXP_004505"
     CDS             complement(1233..2336)
                     /gene="yhdY"
                     /locus_tag="ACHKXP_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7649368.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQYLEKRFNTGRTPH"
     gene            complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHKXP_004506"
     CDS             complement(2346..3527)
                     /gene="yhdX"
                     /locus_tag="ACHKXP_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7649369.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3595..4620)
                     /locus_tag="ACHKXP_004507"
     CDS             complement(3595..4620)
                     /locus_tag="ACHKXP_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7649370.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5051..5272)
                     /locus_tag="ACHKXP_004508"
     CDS             complement(5051..5272)
                     /locus_tag="ACHKXP_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649371.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5525..8627)
                     /gene="acrF"
                     /locus_tag="ACHKXP_004509"
                     /pseudo
     CDS             complement(5525..8627)
                     /gene="acrF"
                     /locus_tag="ACHKXP_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8639..9797)
                     /gene="acrE"
                     /locus_tag="ACHKXP_004510"
                     /pseudo
     CDS             complement(8639..9797)
                     /gene="acrE"
                     /locus_tag="ACHKXP_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
BASE COUNT         2693 a         2602 c         2425 g         2391 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcgaaa
      421 ggccatccga aaggatggcc tttttgcttt tcgaactaac attcaattaa tggattacct
      481 gcgataaaaa tgccctcgta cgctctgatt taggatgcgc aaaaaactca tcaggtgcag
      541 cttgttccac tatctcccca cgatccataa aaatcacccg gtcagcgacg gttcgtgcaa
      601 accccatctc atgtgttaca cacagcattg tcatacccga ctgcgccagc ccaatcatcg
      661 tatccagcac ctctttcacc atctcaggat cgagcgccga cgttggctca tcaaacaaca
      721 taattttcgg cttcatacac agcgaacgcg caatggcaac gcgttgctgc tgaccacctg
      781 aaatctgtcc gggaaactta tgcgcatgtt cggcaattct cacccgctct aggtaatgca
      841 ccgccagatc ttcagcctct ttcttaggca tcttgcgtac ccaaatcggt gccagggtac
      901 agttctgtaa aacggtcaga tgagggaaga gattgaaatg ctgaaagacc attcccactt
      961 cctgcctgac gcgctcaata ttgcggatat cttcattaag ttcgatgcca tctaccacga
     1021 tccgtccctg ttgatgttct tccagatgat taatacaacg aatggttgtc gatttaccgg
     1081 aacctgaagg gccacacaga acgatccgtt ctcccggttg cacggttaaa tttatatttt
     1141 tcaaaacatg gaattgtcca taccatttat tgacgttttc cagcgtaatc atcgcgttag
     1201 caggttgcag taaaatttgg ctcatagtgt cctcaatgcg gtgtacgccc ggtgttaaaa
     1261 cgtttttcca gatactggct atagcgcgac atgctgaaac agaagatcca gtagatcagc
     1321 gcggcgaaaa catacccttc cgtcgacata cccaaccagg cgggatcaac ggttgcctgc
     1381 tgaacgctac tgaaaagatc gaacaacccg ataatgatca ccaggctggt atctttgaag
     1441 agtgcgatga tggtatttac cagcccagga attaccagct tcaacgcctg tggcagaata
     1501 accagcccct gagttttcca gtaacccaac gccagcgact ctgccgcttc atattgccct
     1561 ttaggcagcg cctgtaatcc acctcgcacg acttccgcaa catatgctga ctgaaacagg
     1621 atcacgccaa ccagcgcgcg gatcaatttg tcgatactgg tgccttctgc cataaacaac
     1681 ggcagcatga ccgaagacat aaacagaacg gtaatcagcg gcacgccgcg ccagaattcg
     1741 ataaaaatga ccgataagat acgcacaatc ggcatatggg agcggcgacc taacgccagt
     1801 aagatccccc acggtagcgc ccccgcaatc ccaactgatg caataattaa agttagcgtc
     1861 agcccgcccc attgccgggt ttcaacccgc tcaagagcaa aaaaaccgcc atacatcagc
     1921 caccagacaa tcagtgggta aatcaccgcc caggccgcaa tatagcgacc gcgatgcggg
     1981 agtattttcc agaacattgg tgcgatcgac acaagcccaa tcagtaatgc gaggttaatt
     2041 cgccagcgtt ggtcgtgtgg gtaaagccca tacataaact gaccaaatcg ttcgtggatg
     2101 aagacccaac aggcaccggc ttttgtgcag tcggcacgcg ttgagccaac ccagttagcc
     2161 tgcaaaaatg cccagttcag caacggtggg atcagttccc acatcaacca tatgcagcca
     2221 atagtcagca ggctattgct ccagctggag aacagatttt ttcgcaccca caccatcgcg
     2281 cggcttgagt tatggctcgc cgggcgcggg ggatgagaca gcaatacttt tgtcatcatg
     2341 actccttagc gttcaacgat cgcgatgcgg cggttataga tattcatcag cagcgagata
     2401 gtcaggctga taatcagata gaccgacatg gtcatggcta tcgtctcgat ggcttgcccc
     2461 gtctgattca gcacggtgcc ggcaaacagc gaaaccatat cgggatagcc aatagcggcg
     2521 gcaagagagg agtttttgac gatgttgaga tactggctgg ttaacggtgg aataatcacc
     2581 cgcaatgcct ggggaataat gacctggcgt agcgtaaccg ggttgggtaa tcccagtgac
     2641 cgagccgctt catgttgacc ataaggcact gcctggatcc cagcgcggat aatctcggcg
     2701 ataaatgcag atgtataaac cgaaagtgcc agcgttaagg ctgccagttc aggaattaaa
     2761 accatcccgc cgcggaagtt aaagcctcgt agggctggga catcccagtg tagtgctgcg
     2821 ccaaaaagcc attgcgccag taaaggcaaa ccaatgatca acactgcggc gatcggccag
     2881 gtgcggcgca gttgtccggt ctttatctgg tatgttttat taaagcggaa tagcccaaca
     2941 gaaaggacta tagccataac aacagccagg ataaacgcaa taaatccgtc tcccagctgc
     3001 ggtgacggaa tataaagccc acgattgctc aaaaaggcca gatcaaacgc gctcacagcc
     3061 tggcgtggtc cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc
     3121 gggggaatat tacggaagat ctcaatataa attgtggaaa gctttcgtag cagccagtta
     3181 tccgaaagtc tcgccagacc gataaagaag cccaaaacag aagcgaacac aatacacaat
     3241 gcagaaacca gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc
     3301 tgctggtaat cgatcaaatg ctggacaata ccgaagccag caccgcgatc cagaaaggca
     3361 aaacctgaag taatgccacg attattgaga ttcgttacag tgttgtgaaa taaccaacca
     3421 acaatgccga caacagcaac aacggcaagg atctggaata accaggcgcg aaccgtaggg
     3481 ttggcaaaag agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa
     3541 agcataacgc tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg
     3601 cacgggcggt gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat
     3661 tttcagcggg ctttctgaac ctacgttacg ctcaaaaatt tccgagtagt tacccacctg
     3721 tttgatgatg ttataggccc atttattatc cagcttcaga tccttgccgt aatcgccttc
     3781 tttacccagc agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt
     3841 ctgggaattg atgcccatct cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat
     3901 cgagaaccat tcatcatcgc cacgacgaac taccggacca agcggttctt ttgagataac
     3961 ttccggtaag acaatccatt cagctgggtt gcttaatttg atgcgcaggg catacagttg
     4021 tgattgatcc gaggccagcg tatcgcagcg accagattcc agtgccttcg ctgattcgtc
     4081 agagcgatcg aaagtcaccg gtgtgtactt catattgttt gccttgaagt agtcggcgac
     4141 gttgagttcg gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc
     4201 gctttttagc cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc
     4261 ggtaaatgcc attcccatcc cggcatcgcg agatgaagtc caggtcgtat tacgggagag
     4321 caaatccacc tcccctgact gtaaagcggt gaagcgttct tttgcagtga gcggggtata
     4381 tttcactttc gtgtcgtcac caaatacagc agcggcaaca ccacgacaaa tatcaacatc
     4441 aatacctgaa aacttaccgt cagcatcggc ataagagaac ccaggtaatc catcactgat
     4501 cccgcattgc acaaaacctt ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg
     4561 atttgcaacg gcaagcagca cgctggcggc agccagtgtg gctatcatca tctttttcat
     4621 aatgcttcct gtgaggcgaa aattatcgtt ataggacgtt tgggaaacgt cttttacctg
     4681 tctgtggcca tcctcttata agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg
     4741 caataagctg agtgtagaca ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg
     4801 aaacatgcgc cacattttgg cgcaaaatat ttacgcgtgt tttactaagc agaataatga
     4861 ttgttagcgt gagtatattt ctggtgggaa gtgtgaaaat taaattgatg cgttatttcg
     4921 aaagcagcga aataacgcga tgtaacatgc taaatcagca gggaattacc cagcagaata
     4981 tagcaatatt ttatcttatg gagagatact gtatcgttaa aaagagcgcg gaactccgcg
     5041 ccctgttcag ttatttcgat agagcgatga tccccatcgt caggccagca acagcagctg
     5101 ccccgccggc aatagcccca gccgtttccc agttatcctg agtcgtgcct ttcatacagg
     5161 tattggtgtg aaccagacga ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag
     5221 cacagcccgc cagcaatgcg gtgagcaatg ccacaggaat taatcttttc atggtgttac
     5281 ctcagtaata aacgctaacg cttaataagt taactctctt attaacgagc tgaatgccat
     5341 tttatcaccg gaggttataa atcttgcgga atgaataatg acaaattatg tgaattgtaa
     5401 aaatagtgaa gggagcggga actatagaaa gcttttaaag agtgagcaga agaatatctt
     5461 attaattttt atgggtttca ctggaaataa taaaggcacc cgaaagcgcc tttatgtttc
     5521 tgatttatcc tttaaagcaa cggcggatca ccacaaagaa caccggtacg aagaagattg
     5581 ccagcaacgt tgcagagacc attcctccca ttaccccgat acccacagcg ttctgcgcgc
     5641 cactgccggc accgttactg atagctagcg gtaatacgcc gagaataaag gcgagagagg
     5701 tcatcaggat aggacgcaga cgcatacgta ctgccatcag tgtcgcttca acaacacctt
     5761 taccctcttt ctccatgaga tctttagcga actcaacgat caaaatagcg tttttggccg
     5821 acaagccaat tgtcgttagc aagcccacca taaagtagac gtcatttttt tgattaaaga
     5881 gtgttgccgc cagtagcacg ccgacaatcc ctaacggcac taccaacata accgagacag
     5941 gaattgacca gctttcatag agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg
     6001 ctaccagagc gggagcctgg tttcccgata agcgttcctg atacgacata cccgtccagt
     6061 cataaccaat gcccgcaggt aattttgacg caaggttttc catcaacgcc atggcatcgc
     6121 cggaactggt tcctggcgcg gcttccccct gaatctccat tgacggcaga ccgttgtagc
     6181 gttccagtcg cggagagcca tacacccaat gtgaagtggt aaaggccgag aatggcacca
     6241 tttcgccgtt ggcgctgcgg acataaagtt tatcgacatc ttctggcagc atacggaatt
     6301 tggcatccgc ctgaacatac acctttttca cacgaccacg gtcgatgaag tcgttaacgt
     6361 aagtcccacc cagcgccgtt gaaatggtct gattgatgtc agaaagtgaa acacctaatg
     6421 cctgcgcctt ttcctggtca acttccagtt taaactgcgc ggtgtcttcc aggccattag
     6481 gacgcacgct gactaagctg gcaggatgtt gcgcagccat accaagcaac tggttacggg
     6541 cctgggttag ggcatcgtga cccagcccag cctgatcaat taactcaaag tcgaaacccg
     6601 ttgccgtgcc cagttcaaca atggctggca tattgaatgg aatgacaaaa ccgtcgcgga
     6661 tcttgcccaa ttccattttg gcacgatgga ttaccgcttc cgcactgttt tcatcaccat
     6721 tacgctcttc ccacggtttc agactgacga aggccatacc ggcgttttgt gcctggccgc
     6781 tgaagctaaa gccgttaacc gtaaagacac tttcaacgtt cgctttctcg ttcttcagat
     6841 agtaatccgt cacttgatcc aacacttttt gcgtccgctc ttgcgtcgcg ccagcgggta
     6901 actgaatcat ggtcagaaag acaccctgat cctcttcagg taagaaggaa gacggaagac
     6961 gtaaaaacaa caccaccatt cctgcaacaa tcagcgcata gatcagtaaa tatcgtcctg
     7021 tggaaccgag gattttgccg acgctgttgg tgtagtggtt aacgctatga tcgaaggtgg
     7081 tattaaacca gccgaagaaa ccgcccttat tttcgtgatg ctcagcagag acgggtttaa
     7141 gcagcgttgc acataacgca ggggtaagaa tcaatgccac cagaacagaa agcgccattg
     7201 ccgaaacgat ggtgatagag aactggcgat aaattgcccc agtagaaccg ccgaagaatg
     7261 ccatcggaat aaataccgct gacagcacca tcgcgatacc caccagtgcg ccctgaattt
     7321 gcgacattga tttttccgtc gcttcttttg gcgggagctt atcctccatc atcacgcgct
     7381 cgacgttctc caccaccact atcgcatcat cgacgcagcc cgatggcaag caccatcccg
     7441 aacatcgtta gtgtgttgat ggagtaacca aaagcggcga ggatggcaaa cgtccctaac
     7501 aacaccacgg gtaccgcaat ggtggggatc agcgttgctc gcatattctg caagaacaga
     7561 tacatcacca ggaacaccag cataatggct tcgaacagcg tttttaccac ttcgtgaata
     7621 gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa ccttcattcc ctgcgggaag
     7681 aatggctgta attccgccag ttttgcctta atggctttcg cggtatcgag agcattcgcg
     7741 ccggttgcca gcttaatccc caggcccgcc gccggttttc cgttgatacg agcgataacg
     7801 ttatagtttt caccgccaag ttcaacccgt gcgacatctt tcaggcgtac caccgagccg
     7861 tcactgttta cgcgcagggt cactttgccg aattcttccg gatttttaaa ccgcgtctga
     7921 gcaataatcg aggcgttcaa ttgttgccct ggtaacgctg gcgttccgcc caactgtccg
     7981 gcagcgatct gatcgttctg taccttcaac tggttaatca catcaaccgg tgtcagttta
     8041 tatttgttta gcagatcggc atccagccag atacgcatcg catactgtgc gccgaaaagc
     8101 tgtacgtcac cgacgccatt cagacggcta agcgtatctt taacgttaga ggccacatag
     8161 tccgagatat cgtcctgtgt ggtgcctggg ttatcagaga caaagcccgc caccatcaaa
     8221 tagctgctac tggacttttc aacactgatc ccctgctgct gaacctcctg cggcagcaac
     8281 ggcgtggcga gctggagttt gttctgcacc tgcacttgcg cgatatcagg atcggtcccg
     8341 gactggaagg taagggtaat tgtcacgcta ccggcggaat cgctggtgga ggacatatac
     8401 atcaggttat cgataccgtt catattctgt tcgataacct gcgtcaccgt atcctgcacg
     8461 gtctgcgcat cagcgcccgg atagtttgct gaaacagaaa ccgcaggcgg tgcaattgtt
     8521 ggatactgag cgacgggcaa ttgtaggatc gccagtgcgc ccgccatcat cagaataatg
     8581 gccagcaccc atgcaaatat cggtcgtcga ataaaaaagt ttgccatgtc agattacctt
     8641 acttcgatgc agtatctgcg ggggtatcgg tagtggcttt aacctgctct cccggacgcg
     8701 ctttttgcag gccgctgaca atgacttgat cgccagattt cagtccttca ctaatcaacc
     8761 atttatcgcc aatcgcctga ctggcaacga cagggcgcgc ttcaacctga cttttatcgt
     8821 taacaatcag cacggttgca tcaccacgcg gtgtgcggct aacgccttgt tgcgggataa
     8881 gaatggcgtc aggttggacg ccttcatcaa tccgtgcacg cacaaacata cccggcaaaa
     8941 gcgtatgttg cgggttaggg aagacagcac gtagggttat ggagccggtg ctttcatcaa
     9001 cggtcacatc ggagaattgc agcgtacctt tcaggggata ggtttgaccg ttttccatga
     9061 ccaactctac gttgctggtg gcgttttcct tatgcaaatt tccttgctct acggattgct
     9121 tcagcctcat aaaatcgttg ctggattggg tcacatcaac gtagatagga tcgagctgct
     9181 ggacagtcgc cagttcagtc gtttgcccat tagtgacaag agcgccttcg gtcacagtcg
     9241 atttgccgat acgtccgcta attggcgcag tgactttggt ataagcaaga ttgatgcgag
     9301 cgctttcgac tgtggctttt gcggcaatca cggcggcatc ggcctgacga gcatcagcaa
     9361 tggcctggtc gtactcctgc tggctgatgt atttcgtacc cacgagcgga acgtaacgtt
     9421 ttaccgtcaa atgcgcgatg gcggcggcgg cttcactttt cgccagttcg cctttcgcgc
     9481 tgtcataatt tgcctgatag gtcgcgggat cgatctggta cagggactgg cctgcttgta
     9541 catcgctgcc ttcagtgaaa ttacgattca gtacgatccc gctaacctgt gggcgaactt
     9601 cggctatacg gtaagcattg gtgcggcctg gtaattcagt cttaacttct aacggggccg
     9661 ttttcacaat atgaacggta acctgcggtt caccgacgtg agctttctct tctcccctta
     9721 tcgttacaac cggcgattaa agccgcggag atcagaataa aggagggcag gaggaaaaac
     9781 ctggcatgtt tcgtcattac tattcctcaa aaaaccaaaa gcgcgttatt tacccagaaa
     9841 ggcaacgcgt taactcgcag aaagaaaaat acagtttgct atcctacaaa ttatcattcg
     9901 tcgatgtaag gaatagttat gaatactggc gtctcaaggc acataaacac aaaaaaagat
     9961 taatattcta ctgttttatt ttgacgcggg ttgaaagagg cagaattaaa acctcgtaaa
    10021 ttgaaatata tattcatgtg gcgaatttat tttaggtaaa taatatatat tatttatttg
    10081 acgatttata gcaactcaat gttaccctct c
//
LOCUS       DAZFZC010000145         9840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFZC010000145 DAZFZC010000000
VERSION     DAZFZC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.146"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1007
                     /locus_tag="ACHKXP_004511"
                     /pseudo
     CDS             1..1007
                     /locus_tag="ACHKXP_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            complement(1038..1757)
                     /locus_tag="ACHKXP_004512"
                     /pseudo
     CDS             complement(1038..1757)
                     /locus_tag="ACHKXP_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
     gene            1975..2910
                     /gene="dsdC"
                     /locus_tag="ACHKXP_004513"
     CDS             1975..2910
                     /gene="dsdC"
                     /locus_tag="ACHKXP_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HFO7649372.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHHFLMDEEILPVCSPEYAQRHALTNTVINLCHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            complement(3006..3080)
                     /locus_tag="ACHKXP_004514"
     tRNA            complement(3006..3080)
                     /locus_tag="ACHKXP_004514"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3045..3047),aa:Arg,seq:cct)
     gene            3276..4445
                     /locus_tag="ACHKXP_004515"
     CDS             3276..4445
                     /locus_tag="ACHKXP_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7649373.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPVFTQ
                     VKPYIERLLNAGFDVLFPGQEIDKPIDRSSANLYMKKLREKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            complement(4429..4611)
                     /locus_tag="ACHKXP_004516"
     CDS             complement(4429..4611)
                     /locus_tag="ACHKXP_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7649374.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(4690..5058)
                     /locus_tag="ACHKXP_004517"
     CDS             complement(4690..5058)
                     /locus_tag="ACHKXP_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7649375.1"
                     /translation="MNAVLTELNKLGKATADSISKGLNIDLNDVIDTLWKLKNQGIVK
                     VKNGIWQAVEGVGSKTDIDSVQPVQPVQPVQNNIIGDLLRKSRKEARRAGRKQKRWEG
                     ACKALQELNKYRDLINELSE"
     gene            complement(5094..5252)
                     /locus_tag="ACHKXP_004518"
     CDS             complement(5094..5252)
                     /locus_tag="ACHKXP_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7649376.1"
                     /translation="MEIANQLARSGIRFVPIPITADTELHLFGEILSRKLDELEKLVE
                     EADTSPIA"
     gene            complement(5261..5893)
                     /locus_tag="ACHKXP_004519"
     CDS             complement(5261..5893)
                     /locus_tag="ACHKXP_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO7649377.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKRHHQLN"
     gene            complement(5890..6093)
                     /locus_tag="ACHKXP_004520"
     CDS             complement(5890..6093)
                     /locus_tag="ACHKXP_004520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649378.1"
                     /translation="MSDLAMKVLKWQSTDYVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVALYIDEFENAL"
     gene            complement(6120..6287)
                     /locus_tag="ACHKXP_004521"
     CDS             complement(6120..6287)
                     /locus_tag="ACHKXP_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649379.1"
                     /translation="MKFKFTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHAEVTNI
                     RIEELKEHQAA"
     gene            complement(6330..6953)
                     /locus_tag="ACHKXP_004522"
     CDS             complement(6330..6953)
                     /locus_tag="ACHKXP_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO7649380.1"
                     /translation="MTSQLIPVFNGTIDNETILLVNARDLHSFLGVGRMFAHWVKERI
                     AEYGFVESQDYILICQNGQTKGRGGDRRSKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMEKAQDISKRLYPILKELNSSYASKLYD
                     IAFETFYTVTKNRDALLREATRIDQTSAIFERARPMLKSLRARQFEF"
     gene            complement(7309..7592)
                     /locus_tag="ACHKXP_004523"
                     /pseudo
     CDS             complement(7309..7592)
                     /locus_tag="ACHKXP_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
     gene            complement(7592..7972)
                     /locus_tag="ACHKXP_004524"
     CDS             complement(7592..7972)
                     /locus_tag="ACHKXP_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7649381.1"
                     /translation="MINRTKLEHILEYARQQRHTGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTTDGWISCSDRMPEKGQNVLISVNFDSDLVEPLICSARYTGSTFRRGQITVAPGN
                     GIDGNGIEQATHWMPLPEPPQEVN"
     gene            complement(7969..8424)
                     /locus_tag="ACHKXP_004525"
     CDS             complement(7969..8424)
                     /locus_tag="ACHKXP_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /protein_id="HFO7649382.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALAALE
                     AEPVLYQSCTRPTWNSGVPWTEWKERSREGYENDLRFTDTPDHAGWINKCRKLYTTPP
                     VPVIQADVAQAIENLKQKLVECNRYNYCADAVKGVEDACRAAMLKGDKS"
     gene            complement(8421..8630)
                     /locus_tag="ACHKXP_004526"
     CDS             complement(8421..8630)
                     /locus_tag="ACHKXP_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649383.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            complement(8632..8820)
                     /locus_tag="ACHKXP_004527"
     CDS             complement(8632..8820)
                     /locus_tag="ACHKXP_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649384.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAEQNRWKNHLRTKGE"
     gene            complement(8972..9745)
                     /locus_tag="ACHKXP_004528"
     CDS             complement(8972..9745)
                     /locus_tag="ACHKXP_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7649385.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDDQAYANAEFIAEANPATVLALLDERERNQQYIKRRDQEN
                     EEIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSENAY
                     IRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQ
                     AYFNRKYAPIDEKLMALHKWFWEQSEAERAAGIRIKGGE"
     gene            complement(9742..>9840)
                     /locus_tag="ACHKXP_004529"
     CDS             complement(9742..>9840)
                     /locus_tag="ACHKXP_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7649386.1"
                     /translation="IDERRRLVVQGCRTCASCQEDLELISKQRGSK"
BASE COUNT         2407 a         2385 c         2300 g         2748 t
ORIGIN      
        1 cagcaaaatc agcagacgtc ttctgaacgt gaactggaga tgtctaccga gattgcacgt
       61 ctcaaacgcc agctggcaga acgggatgaa gagctggcta tcctccaaaa ggccgcgaca
      121 tacttcgcga agcgcctgaa atgaagtatg tctttattga aaaacatcag gctgagttca
      181 gcatcaaagc aatgtgccgc gtgctccggg tggcccgcag cggctggtat acgtggtgtc
      241 agcggcggac aaggataagc acgcgtcagc agttccgcca acactgcgac agcgttgtcc
      301 tcgcggcttt tacccggtca aaacagcgtt acggtgcccc acgcctgacg gatgaactgc
      361 gtgctcaggg ttaccccttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcagggac
      421 tgagggcaaa ggcctcccgg aagttcagcc cggtcagcta ccgcgcacac ggcctgcctg
      481 tgtcagaaaa tctgttggag caggattttt acgccagtgg cccgaaccag aagtgggcag
      541 gagacatcac gtacttacgt acagatgaag gctggctgta tctggcagtg gtcattgacc
      601 tgtggtcacg tgccgttatt ggctggtcaa tgtcgccacg catgacggcg caactggcct
      661 gcgatgccct gcagatggcg ctgtggcggc gtaagaggcc ccggaacgtt atcgttcaca
      721 cggaccgtgg aggccagtac tgttcagcag attatcaggc gcaactgaag cggcataatc
      781 tgcgtggaag tatgagcgca aaaggttgct gctacgataa tgcctgcgtg gaaagcttct
      841 ttcattcgct gaaagtggaa tgtatccatg gagaacactt tatcagccgg gaaataatgc
      901 gggcaacggt gtttaattat atcgaatgtg attacaatcg gtggcggcgg cacagttggt
      961 gtggcggcct cagtccggaa caatttgaaa acaagaacct cgcttaggcc tgtgtccata
     1021 ttacgtgggt aggatcacgc aatgggtagc agtatggtga ataacgttgc gcctaatgac
     1081 ggtagtgttt tttcatcgcg aactttgaga tctgcaaact ctgtgggtac aggtttaaag
     1141 ggcagtcgtt gacccagaaa tttaaggaaa agtgggccac cgatcagtga tgccatcagc
     1201 ccaaccagca aaccgtagac gatcaccgaa ccgatatctg cgcccagctt attggcaaca
     1261 tataaagcag ccggatgtgg aggaaccacg cagtgcactg ccatcaatgc ggtacatagc
     1321 ggaatggcaa gctttaataa tgaggtattg gtttttttgg caattgaaaa agccagagga
     1381 atcaatagca cgacgcccac ttcaacaaac agcgtgatgc cacaaatcag gccaaccagc
     1441 accataatga catcaactga aagccagcgg cagcgttgaa gtgtcagacc aattctttct
     1501 gcggccccgg atacttccat catttttccc agtatcgtgc caaggccgat aaccgctgcg
     1561 aggaacccca gcgttccgcc aattccactt tcaatagcat ttaccatatc cagtggcccc
     1621 atgcccatca tcgttcccac gaagaagctg gccagcaaca gcgccagaaa cgggtggaat
     1681 ttgaacttca cgatggtcag tacaattaac acgatgctga taagcagcgt gctcacaacc
     1741 cagatttgag agtgcatatc tcaccttccc ctgtgattaa cctgatgata ttggacaaaa
     1801 aaacaacgca ggctgacaaa cgataaaatt tgcctttcac atgaggtaaa ttgcatcaat
     1861 ggagtgattt acatctaaaa atgaaggtga attgagatat ggttcacttt agctcatctc
     1921 atagctgaaa ttttgccgct atttttttac acttaagcga aaaatgaggt gattatggaa
     1981 ccccttcgtg aaataagaaa tcggctgctt aacggctggc aattatcaaa aatgcatact
     2041 tttgaagtgg ctgccaggca tcagtccttc gccctggcgg cagaggaatt gtcgctgagc
     2101 cccagtgcgg taagtcaccg tatcaatcag ctggaagaag aattgggcat tcagttgttt
     2161 gttcgttccc atcgcaaagt ggaattaacg cacgagggga aacgtgttta ttgggcgcta
     2221 aaatcgtcgc tggataccct gaaccaggaa attcttgata tcaaaaatca ggagttatcg
     2281 ggaacgttaa ctctgtattc ccggccctct atcgcccaat gctggttggt gcccgcatta
     2341 ggtgacttta cacgccggta tccgtctatt tcgctcaccg tgctcactgg taatgacaac
     2401 gtcaatctgc aacgtgccgg aatcgatttg gcgatctact ttgatgatgc gccgtcagcg
     2461 caactcactc atcactttct gatggatgaa gaaatcctgc cagtatgtag cccggaatac
     2521 gctcaaagac atgctttaac caacacggta attaacctgt gtcactgtac gttgctccat
     2581 gacagacagg catggagcaa cgactccggt acggatgaat ggcatagttg ggcgcaacat
     2641 tatgcggtta atttgccgac atcttctgga attggctttg atcgttctga tttagctgtt
     2701 attgccgcga tgaatcatat tggggtggcg atgggaagaa aacgcctggt acaaaaaagg
     2761 cttgccagtg gtgagctcgt cgcgccgttt ggcgatatga cggtgaaatg ccatcagcat
     2821 tattacatca ccacattacc gggcaggcag tggccaaaaa ttgaagcgtt tattacctgg
     2881 ctaagagaac aggtaaaaac cacttcataa tgaataaaac cacgagttcc tggtgtgcct
     2941 tattcggcct gctagttcga atttaattta cggtttttat gtggttttag ggataaacac
     3001 aaggatggtg tcccctgcag gaatcgaacc tgcaattagc ccttaggagg ggctcgttat
     3061 atccatttaa ctaagaggac attgttgcgt attgattttg gttgttcacc atatgactga
     3121 taataccgtc tttatggttt taaaatcaag gtgtaagttt gtttttgttc atggttgatt
     3181 atcttctttt gcgttgaaaa gtgttttgtt cacttgctaa tgcgtacata ttgagtacag
     3241 aatacattat aaacttgtgt acaggatata aagcattggc actgagcgac accaaactac
     3301 gaggcctcta cggaaaaccc tactctggcc ctgctgaaat caccgatggt gacgggttaa
     3361 gtgtgcggat tactccagca ggaactatca cgtttcagta ccgttatcgc tggaatggta
     3421 agccagtacg tcttactgtc gggcgctatc cgtctacttc actgaaggac gcgcgtgtta
     3481 tcgtcggtga gatgcgcgca ttgtacatga agggagttaa ccctaaaaat tattttgcgc
     3541 ccagtgacgg cgaactgaca ttaaaagaat gcctggatca gtggtgggat aagtatgtta
     3601 ctgatctgaa acccaataca cagacgcttt acagatccgt cgtgtacaac actatgtaca
     3661 cacagtttga aggctcgcca gttgccagta tcccagtatc ggcatgggct cgtttctttg
     3721 acaaacaaga aagcctcaac aagaaaaagg ctcgtgtttt gttgttacaa ctgcgttcag
     3781 ttattaactg gtgtatcagc cgccagttaa tcccatcatg cgaattactc aagcttagtg
     3841 ttaagaatat tgggaaaaaa ccagatgtag ggagtcgtgt acttacctat acggaactgg
     3901 caaaaatttg gctggcgctg gagaactcaa aagtagttac ttccaacaag gtgttacatc
     3961 aattgctatt gctatggggg gccagacttt ctgagctacg tctcgcgaca gccagtgagt
     4021 tcaacatgga agatttggtc tggacaaccc caaaagagca ttcaaaaatg gggaatatta
     4081 tccggcgtcc ggtattcact caggttaaac cttatatcga gagattgctt aatgcgggtt
     4141 ttgatgtgct attccccggg caggaaatag ataaacctat tgatcgttcg tcggctaatt
     4201 tgtacatgaa aaagttaagg gagaaaattg atattcctga atggagaacg catgatttta
     4261 ggcgttctct ggtaacgaat ttatctgggg agggaattat gccccacgtc actgaaaaaa
     4321 tgctggggca tgaacttggg ggagttatgg ccgtgtataa taaacacgac tggttgtcgg
     4381 aacagaaaga tgcgtatgag ttgtatgctg ataaaatttt ctggcacgct aaacagctcg
     4441 gttaattcct ccggctttaa gccattgttc aacggcctga cgactatatc gcgccggatg
     4501 agtaagtact ggttccggga atccgtgttt ttttcgcaaa ttatacaaag ctgtgcgcct
     4561 tttttgtaac aaatcagaaa cctctttttc ggtcattaag ttgacttcca tagtatgcgc
     4621 tccttttcta catgtatttc aacagcctga tatcatccac gccctaaagg acgtggattc
     4681 ccgctacgtt cactctgaca attcgttgat caagtcacgg tatttattca gttcttgcaa
     4741 cgccttacat gcaccctccc atcgtttctg tttccgcccg gcgcggcgcg cttctttccg
     4801 tgatttacgt agcaggtcac ctataatgtt gttctgcact ggctgaactg gttgaactgg
     4861 ttgaactgaa tcaatatctg ttttgcttcc aactccctct actgcctgcc agatgccgtt
     4921 ttttaccttt acgatcccct ggtttttcaa cttccacagc gtatcaatga cgtcattcaa
     4981 atcaatattg agacctttag aaatgctgtc ggctgttgct tttcctaatt tgttcaattc
     5041 tgtgagtacg gcgttcattt ttttctcctg atattttaat gtcggtgggg gtgttatgcg
     5101 attggtgagg tgtcagcttc ttctaccagc ttttccagtt catccagctt tcgggaaaga
     5161 atttcaccaa acagatgaag ttctgtatct gctgtgatcg ggatcggaac aaagcgtatt
     5221 ccacttctgg caagttgatt tgcgatttcc aggcactgcc ttaattcaac tggtgatgcc
     5281 ttttgtcaac gtcgtttttt cgctcattgg ttttctccag aaatttaaca atacccggaa
     5341 ccatctcaat ggatggtgtt ccgcattggt taccccagac atcaaatcca tgcgaggtgt
     5401 gacgggcgaa cagttctatc cggggaacat cgccaagaag ttgcacaagt ttttctcgcg
     5461 ccatatccgg cttgcgggag tgatcaaggc gcggtgcagt gaatgactgg attattccgg
     5521 cgttaatgcg ttcaggcaga tttcctttca ctgcaaaaag gcagtcttcg ctgttagcgc
     5581 gagtggtgct acccataccc atgaccagct tgtcggtctg tcttttcccg catttgttcc
     5641 aggttaatcc tttcatcgtc accagacgaa agccccacgc ttctacaacc ttcaatgctt
     5701 caagtggttg tgtaggcacc caccacatag ccaacaggca attatcggct gccagttccc
     5761 ataccgggag gcggcagata tccagaagac tcatgaccgg atatttaaaa cttgcaccgc
     5821 gttgaccatc ggttgctttg tctcggaatg tccagggggg atctgcgtaa atgagagtgt
     5881 attttttcgt catagcgcgt tttcaaactc atcaatgtaa agtgcaacaa gtgcaacgca
     5941 tcgtctgaaa tcggctgcgt catgtggagc accgaagcta tgaccataga tattcttttt
     6001 cagtccacat gcgattgagg caagagttgc gctactgatg ccaacatagt cagtcgattg
     6061 ccatttcaaa accttcatag ccaaatctga catttcttgt ctccaataaa aaaccgccat
     6121 caggcggctt ggtgttcttt cagttcttca attcgaatat tggttacttc tgcatgtgct
     6181 atctgcgccc atatcatcca gtggtcatag cagtcattga tattctccgc ttcgataact
     6241 ctgttgaatg gttctccatt ccattcacct gtgaatttga atttcattaa gtttcacctg
     6301 taaaaaaagg agccgaagct cctttgatat taaaattcga attgtctcgc ccgaaggctt
     6361 tttaacattg gtcttgcccg ttcgaaaatg gcgcttgtct ggtcaattcg tgttgcctcc
     6421 ctgagcaatg cgtctctgtt tttcgtcacc gtgtagaaag tctcaaacgc gatgtcatac
     6481 agcttgctcg cgtatgatga gttcagttcc ttcaatatcg gatacaggcg tttgctgatg
     6541 tcctgtgctt tctccatctg tacctgcatg tagcagagga ggatgatttc ctcgtctgtg
     6601 aattgctgtg ctggttgcat gctgcgaagt ttcttttcgc actcgatgaa gtatcggcgt
     6661 atctggcggc ctttttcgtt acgctcaacc atcgccagtt ctttggctgt gtcgagagtt
     6721 aggtggtagt ccttgctacg gcgatcacct cctcgccctt tggtttgccc attttggcaa
     6781 atcaaaatat agtcctggct ttcaacgaat ccatattcag caatgcgttc tttaacccag
     6841 tgcgcgaaca ttctgcccac accaagaaaa gagtgtagat cacgagcgtt aactagaaga
     6901 attgtttcgt tgtcgatagt gccgttgaat acggggatga gttgactggt catgatgacc
     6961 tcctttgaag ttttagttag caatcaccag ttagtggctg gtgatcgggt gtcaactaga
     7021 gccttcaaag atgctccggg catattcccc ttgcgggtat tgtattacgc ctctccaccc
     7081 gacctttgta cggatgtgac tatgccaaat tgcaggcata aaaaagccgc aaagctatcg
     7141 ggtgcggatg accgctttga agttctagtg cggtcagtat gcgatagctc tggcggattt
     7201 gtcaaatcat gtaggcctta tcttgctgta agccgcgcca ttcgggcttt tccccagatt
     7261 tgggggaaac tatctgcgaa gtattcacct ttaacggcaa gttgcaggtt agccacgacc
     7321 ttcctccttt ggcttgtgaa tttgtatcgt catgtcgctt tgagtggtga ctacaatgat
     7381 agaaccaggc tgaaggctgt taagattgaa tgcttcgtaa aacgaatcca aggccagcgc
     7441 ttttttattc tttcggttcc accaacgcca tcccttgcta caggctacac tgacaatcca
     7501 ctgtccactc ctgtaagcca tataaaccag atgagcaaaa cctgaaggaa ggcaatccag
     7561 tcaataatcg tatatttcgc gaaggagtcc atcaattaac ctcctgcggc ggttctggta
     7621 gcggcatcca gtgtgtggcc tgttcaatac cattaccatc aataccatta ccaggcgcaa
     7681 ccgttatctg cccacgccga aaggtgcttc cggtatagcg tgcggagcat attagcggct
     7741 caaccagatc actatcgaaa ttcaccgaaa taagcacgtt ctggcccttt tcaggcattc
     7801 gatcactaca gcttatccag ccatccgtag ttaccggaga attgccagcc ttgcgcatgg
     7861 caatctccat gatttcaacc atatcccctg gtggaatttt acaatgctga ccagtatgcc
     7921 tctgctgtct ggcatattcg aggatgtgct ccagcttggt acgattaatc atgatttatc
     7981 tcccttaagc atggcagcgc ggcaggcatc ctctacgccc ttaactgcat ctgcgcagta
     8041 gttatagcga ttgcattcca ctaacttctg cttgaggttt tcaattgctt gcgcgacatc
     8101 agcctgtatt accggaactg gcggagtggt atatagtttt cggcatttgt ttatccaacc
     8161 ggcatggtca ggcgtgtctg taaagcgcaa atcgttttcg tagccctcac gactacgttc
     8221 tttccattcc gtccacggaa caccgctatt ccaagtgggg cgagtgcagg actgatacag
     8281 aacaggttca gcttccagcg ctgccagagc aatccgtgcc agttcttccg cttcttctgc
     8341 tggcagtaca acgttgctac caggtccgta tgtttcgcgc cactgcttga ttgtcagcag
     8401 tcgctctttg gtaatagtgg tcatttgtta atcctcaaaa ctttatgccc gggcgcaaaa
     8461 gcacgcgttt tgtctttgct tattcgccag ccatccttgc gcgcctcttt tgcacagcca
     8521 gcccatgacg tacctatata ctcaccgaag tctggcgact tatatttgcc atctgtacac
     8581 tggaggcaat cacaatagag atgcatggtg taacttgcag caatagccat atcactctcc
     8641 tttagtgcgc aagtggtttt tccagcggtt ttgctccgcg ctgggctttt tgcaaaaacc
     8701 acaatccatc atcccgtaat atttcatcaa ccccatccgt cggttgctga gtctcaccca
     8761 ctgccagacg ccaggagcgt ttctacgaac taacagaatc tttgctttac ggtttttcat
     8821 cgttttgctc tcctgcatct ctttgctgct cgtcgtgccg ctgcaatacc ggtatggcgg
     8881 cgctttggtg tcgggatgat gttctttgca attagcgcag aagcccaaaa acgagtcgga
     8941 tacggtaaca agccgataca tgccacacgc attactcacc tcctttgatg cgaatgccag
     9001 cggcgcgctc ggcttcactt tgttcccaaa accacttgtg aagcgccata agcttttcgt
     9061 caatcggtgc atatttgcga ttaaagtagg cctgagcatc tttctcagat tcgtccggta
     9121 attcgccagg gccaaacagt gtgttataaa tccatgctag tccgctctta gcgtcgccag
     9181 ttgcctgcca ttcgataatg gcagcctgca tgaccagaat gtttttcccg attaataggt
     9241 ccagttcttt gtaccggttg cggatgtatg cattctcgct ttgtaatttt gcgttgcgct
     9301 tttctgaggc ttcaagtaac gcctgcttat cgcgtagagc ttcttccagt tcagcaacat
     9361 ggcattcact atcaataagg ttgttctctg ctgcttcaag ctcaacacgc agcttcccaa
     9421 ccgtaagcgc aatctcctcg ttctcctggt cgcggcgttt gatgtattgc tggtttcttt
     9481 cccgttcatc cagcagtgcc agcacggtag ccgggttagc ctctgctatg aattcagcgt
     9541 ttgcataagc ctgatcatct gattcaatca ggcagttaac atgacattcc gcaatcacgc
     9601 caccgggttc tcctttccat ttttggcaaa caaaaactcc tgttaaattg ccgtgctggt
     9661 taacagatgt atgccctacg atgtagcttc ctttagttgc tttctctgcc ttttcacgca
     9721 gtgcctgata attaatttcg ctcacttcga acctctctgt ttactgataa gctccagatc
     9781 ctcctggcaa cttgcacaag tccgacaacc ctgaacgacc aggcgtcttc gttcatctat
//
LOCUS       DAZFZC010000146         9729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFZC010000146 DAZFZC010000000
VERSION     DAZFZC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.147"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            182..1159
                     /gene="glaH"
                     /locus_tag="ACHKXP_004530"
     CDS             182..1159
                     /gene="glaH"
                     /locus_tag="ACHKXP_004530"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO7649387.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1178..2446
                     /gene="lhgO"
                     /locus_tag="ACHKXP_004531"
     CDS             1178..2446
                     /gene="lhgO"
                     /locus_tag="ACHKXP_004531"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO7649388.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQD
                     RGGEIIYNAEVSALNEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2469..3917
                     /gene="gabD"
                     /locus_tag="ACHKXP_004532"
     CDS             2469..3917
                     /gene="gabD"
                     /locus_tag="ACHKXP_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7649389.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDNGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3931..5211
                     /gene="gabT"
                     /locus_tag="ACHKXP_004533"
     CDS             3931..5211
                     /gene="gabT"
                     /locus_tag="ACHKXP_004533"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO7649390.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5449..6849
                     /gene="gabP"
                     /locus_tag="ACHKXP_004534"
     CDS             5449..6849
                     /gene="gabP"
                     /locus_tag="ACHKXP_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO7649391.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6870..7532
                     /gene="csiR"
                     /locus_tag="ACHKXP_004535"
     CDS             6870..7532
                     /gene="csiR"
                     /locus_tag="ACHKXP_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO7649392.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7533..7982)
                     /gene="kbp"
                     /locus_tag="ACHKXP_004536"
     CDS             complement(7533..7982)
                     /gene="kbp"
                     /locus_tag="ACHKXP_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO7649393.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8066..8224)
                     /locus_tag="ACHKXP_004537"
     CDS             complement(8066..8224)
                     /locus_tag="ACHKXP_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO7649394.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8407..8706
                     /locus_tag="ACHKXP_004538"
     CDS             8407..8706
                     /locus_tag="ACHKXP_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7649395.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8716..9240
                     /gene="ygaP"
                     /locus_tag="ACHKXP_004539"
     CDS             8716..9240
                     /gene="ygaP"
                     /locus_tag="ACHKXP_004539"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO7649396.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9287..9691)
                     /gene="stpA"
                     /locus_tag="ACHKXP_004540"
     CDS             complement(9287..9691)
                     /gene="stpA"
                     /locus_tag="ACHKXP_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO7649397.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQHELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2216 a         2517 c         2683 g         2313 t
ORIGIN      
        1 tcctgtctca ctttgcctta ataccctacg ttaaatgtta ctaatttgtt gcttttgatc
       61 acaataagaa aacaatatgt cgcttttgtg cgcatttttc agaaatgtag atatttttag
      121 attatggcta cgaaatgagc atcgccatgt caccctacat ctcataagag gatcgcttct
      181 gatgaatgca ctgaccgccg tacacaataa cgctgtcgat tcaggccagg actatagcgg
      241 attcactctc atcccgtcgg cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca
      301 gacgaccaaa cagtttctcg aacaggttgc cgaatggccc gtgcaggcgc tggagtacaa
      361 atcgtttctg cgttttcggg taggcaaaat tcttgacgat ctgtgtgcga atcagctgca
      421 accattgctg ttgaagaccc tgctaaaccg tgctgaaggt gcgctgttga tcaatgcggt
      481 gggtatcgat gatgtggcgc aggcagatga gatggtgaag ctggcaacgg cggtagcgca
      541 tctgattggt cgctcaaatt tcgatgcgat gagcggtcag tattacgcgc gtttcgtggt
      601 gaaaaatgtc gataactcag acagctatct gcgtcagccg caccgcgtga tggaactgca
      661 caacgacggc acgtatgtcg aagagatcac cgattacgtg ctgatgatga aaatcgatga
      721 gcaaaacatg cagggtggaa attcgttgct gctgcatctc gatgactggg aacatctgga
      781 ccactatttc cgccacccgc tggcgcgtcg cccaatgcgc tttgccgcgc cgccgagtaa
      841 aaacgtcagc aaagatgttt tccatccggt gttcgacgtt gatcaacagg gtcgcccggt
      901 gatgcgttat atcgaccagt tcgtccagcc aaaagacttc gaagaaggcg tgtggttgag
      961 cgagctttcg gacgccattg aaaccagcaa aggcattctt tctgtgcccg ttcccgttgg
     1021 caaattcctg ttgattaaca acctgttctg gctgcacggt cgcgaccgct ttactccgca
     1081 cccggatctg cgccgtgaac tgatgcgtca gcgtggctat ttcgcttacg ccactcacca
     1141 ctaccagacg catcagtagc gcaaaggaat tgagcggatg tatgattttg tgattattgg
     1201 cggtggcatc atcggcatgt cgaccgccat gcaactgatt gatgtctacc cggacgcccg
     1261 cattgcgttg ctggaaaaag agtccggccc ggcctgtcac cagacgggcc acaacagcgg
     1321 cgtgatccat gccggggttt attacacgcc cggtagcctg aaggcacagt tttgcctggc
     1381 gggaaaccgc gccactaaag ccttttgcga tcaaaacggc attcgctatg acaactgcgg
     1441 caagatgctg gtcgccacct ctgaactcga aatggaacgg atgcgcgcgt tgtgggaacg
     1501 cacggcggca aacggtatcg agcgcgagtg gttaaacgcc gatgaactgc gcgagcgcga
     1561 accgaatatc accgggctcg gcggtatttt tgtgccgtcc agcggcattg tcagctaccg
     1621 cgaagtgaca gcggcgatgg caaaaatctt tcaggacaga ggcggcgaga ttatttataa
     1681 cgccgaagtc agcgccctta atgagcataa aaacggcgtg gtgatacgta cccgtcaggg
     1741 cggtgaatat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
     1801 gaaaatgctc ggactcgaac cgggctttat catctgcccg ttccgtggcg agtatttccg
     1861 ccttgcgccg gagcataacc agattgttaa ccacctgatt taccccatcc ccgacccggc
     1921 aatgccgttt ttgggcgtgc atctcacccg catgatcgac ggcagcgtga ccgtcgggcc
     1981 aaacgcggtg ctggctttca aacgtgaagg ctatcgcaag cgcgacttct catttagcga
     2041 cacgctggaa attttgggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
     2101 aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa
     2161 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca
     2221 ggcggtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac
     2281 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
     2341 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
     2401 agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg
     2461 acgtaacaat gaaacttaac gacagtaaat tattccgcca gcaggcgttg attaacgggg
     2521 aatggctgga cgccaacaat ggcgaggtca tcgatgtcac caatccggcg aacggcgaca
     2581 agctgggtag cgtacccaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca
     2641 accgcgctct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca
     2701 actggttcaa tttgttgatg gagcatcagg acgatttagc gcgcctgatg accctagaac
     2761 agggcaaacc gctggctgaa gcgaaaggcg aaattagcta cgccgcctcc tttattgagt
     2821 ggtttgctga agaaggcaaa cgcatttatg gcgacaccat tcctggtcat caggccgata
     2881 agcgcctgat tgttatcaag cagccgattg gcgtcaccgc agctatcacg ccgtggaact
     2941 tcccggcggc gatgattacc cgcaaagccg gtccggcgct ggcggcaggc tgcaccatgg
     3001 tgctgaagcc tgccagtcag acgccgttct ctgcgctggc gctggcggag ctggcgattc
     3061 gcgcgggcat tccggctggg gtatttaacg tggtcaccgg ttcggcgggc gcggtgggta
     3121 acgaactgac cagcaacccg ctggtgcgca agctgtcgtt taccggttcg accgaaattg
     3181 gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     3241 gcaacgcgcc gtttatcgtc tttgacgatg ccgacctcga caaagccgtg gaaggtgcgc
     3301 tgtcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgc ctgtatgtgc
     3361 aggacggcgt gtatgaccgt tttgccgaaa aattgcagca ggcagtgagc aaactgcaca
     3421 tcggcgacgg gctggataac ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     3481 caaaagtgga agagcatatt gccgatgcgc tggagaaagg cgcgcgcgtg gtttgcggcg
     3541 gtaaagcgca cgaacgcggc ggcaacttct tccagccgac cattctggtg gacgttccgg
     3601 ccaacgccaa agtgtcgaaa gaagagacgt tcggccccct cgccccactg ttccgcttta
     3661 aagatgaagc tgatgtgatt gcgcaagcca atgacaccga gtttggcctt gccgcctatt
     3721 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcgctggag tacggcatcg
     3781 tcggcatcaa taccggcatt atttccaatg aagtggcccc gttcggcggc atcaaagcct
     3841 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     3901 tgtgcatcgg tctttaactg gagaatgcga atgaacagca ataaagagtt aatgcagcgc
     3961 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     4021 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     4081 gcggtgctca ataccgggca cctgcatccg aaggtggtgg ccgcggtgga agcgcagttg
     4141 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     4201 gagattatga atcagaaggt gccgggcgat ttcgccaaga aaacgctgct ggttacgacc
     4261 ggttccgaag cggtggaaaa cgcggtaaaa atcgcccgcg ccgccaccaa acgtagcggc
     4321 accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc
     4381 aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt
     4441 tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat ccaccggatc
     4501 ttcaaaaatg atgccgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc
     4561 gaaggcggtt tctacgcctc gtcgccagcc tttatgcagc gtttacgcgc tctgtgtgac
     4621 gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg
     4681 ctgtttgcga tggagcagat gggcgttgcg ccggatctta ccacctttgc gaaatcgatc
     4741 gcgggcggct tcccgctggc gggcgtcacc gggcgcgcgg aagtaatgga tgccgtcgct
     4801 ccaggcggtc tgggcggcac ctatgcgggt aacccgattg cctgcgtggc tgcgctggaa
     4861 gtgttgaagg tgtttgagca ggaaaatctg ctgcaaaaag ccaacgatct ggggcagaag
     4921 ttgaaagacg gattgctggc gatagccgaa aaacacccgg agatcggcga cgtacgcggg
     4981 ctgggggcga tgatcgccat tgagctgttt gaagacggcg atcacaacaa gccggacgcc
     5041 aaactcaccg ccgagatcgt ggctcgcgcc cgcgataaag gcctgattct tctctcctgc
     5101 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     5161 cgtcagggtc tggagatcat cagccagtgt tttgatgagg cgaagcagta gcgccgctcc
     5221 tatgccggag gcgacgctgc gcgtcttgtc cggcctacgg ggatccaggt cggataaggc
     5281 gtttacgccg catccgacaa tctgtacgtg aacaggaaga aatctatgtt ggccgggtaa
     5341 ggcggagccg ctctccggca aaaagaatca ataacaatta tacgcgtgac ccggcgcggg
     5401 aaatgtcggg gcgctctccc aagtgacaca ctttcgagag gattcaggat ggggcaatca
     5461 tcgcaaccac atgagttagg cggcgggctg aagtcacgcc acgtcaccat gttgtctatt
     5521 gccggtgtta tcggcgcaag tctgtttgtc ggttccagcg tcgccatcgc cgaagcgggc
     5581 ccggcggtat tactggccta tctgttcgcc gggctactgg tggttatgat tatgcggatg
     5641 ttggcggaaa tggcggttgc cacgcccgat accggttcgt tttccaccta tgccgataaa
     5701 gccattggtc gctgggcggg ctataccatc ggctggttgt actggtggtt ttgggtactg
     5761 gttatcccgc tggaagccaa catcgccgcc atgatcctgc actcatgggt tccaggcatt
     5821 cccatctggt tattttccct cgtcattacc ctcgccttaa ctggcagtaa cttattaagc
     5881 gttaaaaact acggcgaatt tgagttctgg ctggcgctgt gcaaagtcat cgctatcctg
     5941 gcctttattt tccttggtgc agtcgcaatt agcggttttt acccgtatgc cgaagtgagc
     6001 gggatctcaa gattgtggga tagcggcggc tttatgccca acggtttcgg tgcggtatta
     6061 agcgcgatgt tgatcaccat gttctcgttt atgggcgcag aaattgtcac cattgccgcc
     6121 gcggaatccg acacgccgga aaaacatatt gtccgcgcca ccaactcggt tatctggcgt
     6181 atttctatct tctatttatg ttctattttt gtcgtcgtgg cattaattcc gtggaatatg
     6241 cccggactaa aagccgtcgg ttcttatcgc tcggtactgg aattgctcaa tattccccat
     6301 gcgaaattaa tcatggactg cgtgatatta ctttccgtaa ccagttgcct gaactcggcg
     6361 ctgtataccg cgtcaaggat gctctactcc ttaagccgtc gcggtgatgc tcccgcggta
     6421 atgggtaaaa tcaaccgcag taaaaccccg tacgtggcgg tgttactctc caccggcgcg
     6481 gcattcttaa cggtggtggt gaactattac gcgcctgcga aggtatttaa atttctgatc
     6541 gacagctccg gcgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgcgg
     6601 atgcgcaaaa ttctgcgagc agaaggaagc gaaattcgct tgcgaatgtg gctttatccg
     6661 tggctcacct ggctagtcat cggctttatt acctttgtgt tggtagtgat gctattccgt
     6721 ccggcgcaac agttagaagt gatctccacc ggcttattag cgatagggat tatctgtacc
     6781 gtgccaatta tggctcgctg gaaaaagctg gtattgtggc aaaaaacacc cgttcataat
     6841 acgcgctgat catgatcagg agtcacacca tgaccattac gtctctggat ggctatcgct
     6901 ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga tgaaaaatta cgaatgagtt
     6961 tgctgacatc gcgttatgca cttggcgttg ggccgttacg ggaagctctt tcgcaactgg
     7021 tggcggaacg gctggtcacg gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag
     7081 agcaggagct gctcgatatt ttcgacgccc gcgccaatat ggaagcgatg ttagtgagtc
     7141 tggcgattgc ccgcggtggc gatgagtggg aggcagacgt tctcgcaaaa gcgcatctgc
     7201 tgagtaagct tgaggcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     7261 atcaggcgtt tcatacggca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     7321 gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc
     7381 tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     7441 tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     7501 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacttgc
     7561 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     7621 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     7681 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcgacactg
     7741 gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg
     7801 ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tattcacttt atcggcatcc
     7861 ggtataccgg ttttgttcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc
     7921 tgacctgtaa ccgcgtccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     7981 atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt
     8041 taacgccgcg gggatgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     8101 aaccggggat atacccaagc agtgtcaaca gaatattaat aatgaacgcc caaccgaacc
     8161 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     8221 ccatatgtac tccctataag aaaattactc attgtttaaa aagagatttt atctcttaag
     8281 cgtagtaatt ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg
     8341 ctgaggctgg tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa
     8401 ataaatatga ctgaactcgc gcaattacag gccagtgccg aacaggcagc ggccttattg
     8461 aaagcaatga gccaccctaa acgattgctg attctgtgca tgcttagcgg ttcccccggc
     8521 accagcgcgg gagagctgac gcgcattacc ggactgagtg cctctgcgac atcacagcat
     8581 ctcgctcgta tgcgggacga agggcttatc gacagccaac gggatgccca acgcattcta
     8641 tattccatta aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt
     8701 ccgtaaggag tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc
     8761 gcacgcggcg caaagttaat cgatattcgt gatgctgatg aatatcttcg tgaacatatt
     8821 cctgaagcag atctggctcc attatccgtg ctggaacagt caggtcttcc ggctaaatta
     8881 cgtcacgagc aaattatatt ccactgccag gcaggtaaac gcaccagtaa taatgccgat
     8941 aaattagcag cgattgccgc ccccgcagaa atctttttac tcgaagatgg gattgatggc
     9001 tggaaaaaag caggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc
     9061 caggtgcaga tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta
     9121 aatagcggtt tcttcttatt aagtggcttt gttggtgccg ggttactgtt tgcaggaatc
     9181 agcggttttt gtggaatggc aaggttgtta gataagatgc cgtggaacca acgagcttga
     9241 gaagcgacgc cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat
     9301 cgtcgagaga tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac
     9361 cggtccaggt tttagtttca ccgttaacat cggtgaattt atatttcgcc ggacgcggct
     9421 ggcgtttttt accagcgcgt ggtgcagcag cagagctatt acccaataac tcttccgggt
     9481 taattccgtc agctttcatc agctccagcc aggtgctaat tttttcctgg cgctctgcca
     9541 gttcatgctg ctgctgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt
     9601 cgagcatttc ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa
     9661 tgttatttaa actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca
     9721 aaaaaagta
//
LOCUS       DAZFZC010000147         9684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFZC010000147 DAZFZC010000000
VERSION     DAZFZC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.148"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..893
                     /locus_tag="ACHKXP_004541"
     CDS             <1..893
                     /locus_tag="ACHKXP_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO7649398.1"
                     /translation="VFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEP
                     SDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCK
                     QKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVY
                     TCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFG
                     LKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRH
                     SRLGNISPAAFREKYHQMAA"
     gene            complement(1040..3760)
                     /locus_tag="ACHKXP_004542"
     CDS             complement(1040..3760)
                     /locus_tag="ACHKXP_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO7649399.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHDHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM"
     gene            complement(3772..4104)
                     /gene="mobA"
                     /locus_tag="ACHKXP_004543"
     CDS             complement(3772..4104)
                     /gene="mobA"
                     /locus_tag="ACHKXP_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO7649400.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            4336..4671
                     /locus_tag="ACHKXP_004544"
     CDS             4336..4671
                     /locus_tag="ACHKXP_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO7649401.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTTQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKTSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(4757..5605)
                     /locus_tag="ACHKXP_004545"
     CDS             complement(4757..5605)
                     /locus_tag="ACHKXP_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7649402.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENMLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            5954..6150
                     /locus_tag="ACHKXP_004546"
                     /pseudo
     CDS             5954..6150
                     /locus_tag="ACHKXP_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6184..6435
                     /locus_tag="ACHKXP_004547"
     CDS             6184..6435
                     /locus_tag="ACHKXP_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7649403.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(6466..6663)
                     /locus_tag="ACHKXP_004548"
     CDS             complement(6466..6663)
                     /locus_tag="ACHKXP_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649404.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGALEVAREIACRLRDMGWTPE
                     RIQEATGLSGEELKKLFPDEQ"
     gene            complement(6686..7588)
                     /locus_tag="ACHKXP_004549"
     CDS             complement(6686..7588)
                     /locus_tag="ACHKXP_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7649405.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIKQATGLSDDELKKITH"
     gene            complement(7653..7919)
                     /locus_tag="ACHKXP_004550"
     CDS             complement(7653..7919)
                     /locus_tag="ACHKXP_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649406.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(8013..8447)
                     /locus_tag="ACHKXP_004551"
     CDS             complement(8013..8447)
                     /locus_tag="ACHKXP_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO7649407.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIGGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(9176..9676)
                     /locus_tag="ACHKXP_004552"
     CDS             complement(9176..9676)
                     /locus_tag="ACHKXP_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO7649408.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         2314 a         2678 c         2401 g         2291 t
ORIGIN      
        1 gcgtattttg cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat
       61 ttcccattga agcgatgtgt caggtatttg gtgtatccag gagcggttat tacaactggg
      121 tacagcatga accctcagac agaaaacaaa gtgatgagcg gctaaaactg gagattaagg
      181 tggcacatat ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag
      241 agaatggcat catcgttggt cgtgaccgac tggcacgtct tcgtaaggag ctaaggctac
      301 gctgtaagca gaaacgcaag ttcagagcga ctacgaactc gaaccacaat ctgccagttg
      361 cgccaaatct gctgaaccag acgttcgctc ctacagcacc aaatcaggtc tgggtggcgg
      421 acctgacgta tgttgccaca caggagggat ggttgtacct cgctggcatc aaagatgttt
      481 atacgtgcga aattgtcggc tacgccatgg gagagcgcat gacaaaagag ctgacaggta
      541 aagctctgtt tatggcgctc aggagccagc gcccacctgc cgggctaatc caccactctg
      601 atcgaggttc acagtactgc gcatacgatt accgggtcat acaggagcag tttggtctga
      661 aaacatcaat gtcgcgtaaa ggtaactgtt acgacaacgc tccgatggaa agcttctggg
      721 gaacgctgaa aaatgagagc ctgagccact atcgttttaa taaccgggat gaagccatct
      781 cagtaatacg ggaatacatt gagattttct acaatcgtca gcgtcgtcac tctcgtctgg
      841 ggaatatctc cccggcagcc ttcagggaaa aatatcatca gatggctgct taaaaaaaga
      901 acaaatggta gtgtccgcta ttgccagtac acctcagagt taaccgggta taaactcaat
      961 tcagtgacta ctcccgccct tattgacgaa ggttcctgcc gtttttgggc tgaatctttg
     1021 caccatcact tatccgggct tacattccag gtgatggtgg ctcccatgtt ttctcctgcc
     1081 gtaacgtata attatcatca tggctctccg ataaacgggt ctgctgtctg atatattccc
     1141 ggtaatactg ctcactgcgt tgacggtgca aatccacgtc cggacgagta atggtgaagt
     1201 gtccgtttcc ggttattccc gctgcattgt gccaggccac catttctgca aacacatcat
     1261 taaactgctg gtatgaacct tccggctcaa aaccaagctt tccgttgcgg ctgaacagaa
     1321 ctggcgcaat taaatcaagc ttctctgcca gttcatttcg gtccgtgtga tggtagaaca
     1381 caacccgatc tccgtaatcc gtacagacat tttttcctgt ccgcgtatcc ctgaaatgaa
     1441 cacttccatt tttctgaaga cgagcttcaa gttttgcaac attattaaaa agtggcgttc
     1501 ctccgggttc gcagagtacg acacaacgat caacattagt tgtgcgtctt ttatccttat
     1561 tacgcgagcg acgatcacga taatcccagc cacgtaactg cgatactgca gccctgtctc
     1621 cctgagccgc ctgttgttcc acccactgcc ggtaggacaa aggataccac ttaccctccg
     1681 caatcagtga caaccgctct tccttcacac tctccttcag cctgatcagc gcctgcatac
     1741 gctgcacttc cgcaatatgg tcatgcagct tacgcaattg tggatcccgg aactgcacac
     1801 ggatatacgc cttgcgccga cggcaggcag catgaatttc ccgtaaccgt tctccgtaac
     1861 gtaagtcggg tttacgccag tgctccttcc atgcaagata acgcgcacgc aaatcttccc
     1921 gcgcagctgc acgggcttca cggcgctcac gacgaatctc aggatcgcgc cggaagcctg
     1981 gttccacttc atcactggca gcaagttccg gattgtaacg acattcaggc ttcacccgtt
     2041 cgaaaatatc tgcagccgca atttcaaatg gcccggcctg aggctcggca cgggataacg
     2101 tcaaatcagg atggatactg ctggccttca ccggtgtcag ctcatgatta aacgcatcca
     2161 caatcacaag accatgatgc tgtttctgca gcataagccc tttacgcgca aacatctcat
     2221 gacatgcctg ccagctgaaa tccccgtcag ctatcatctc ttcaatcagg ctcctgcact
     2281 gaataaggcc gtatccccac agactttcct tatcagaaaa cagttcgcgc tttattgctc
     2341 cctgtggacc tgtccccatc cgcatacgat accattcgta atcactgaca gcctgagtac
     2401 caagattacg tccccggaac cgttctccgg gtataacctg agtaaaaata tccttcggta
     2461 caggctgcca tccaccatcc agatcctgca tataatccag cgtcattaac ggccacacac
     2521 tgtctgcacg tactggtgtc tgttgtttat caaatccatc atgcagtacc agatgcccgt
     2581 gctggatccc catccataat ccagccgttg ccagagttct gtgcacatcc aggcaacttt
     2641 ccagctcacc atttctcgcc atttccggta agcgttcggc aaaatgacgg caggcgtact
     2701 gtttcagact ctccgtttct gccacttttt ccggccggat atcaacgttt atggcttccg
     2761 gatcataacg accgggtgtc cccacctgac tgaaaatatc tgtcggtaca ggctgatatt
     2821 cccccagttt ccgccccagt ttttcagctg tccataacgg tccgaacgaa ctgagtgcaa
     2881 caccttcccg cgctctgtcc cagccatcct tcaccaccag ttccccttcc tgcatcgtga
     2941 tatacagacc atcactggcc agccgtttat gcaaagataa ccagtcctgt gcgggctctt
     3001 cacgcagccc ggcaatcgac atctgaacaa tatattccct gaacgtctgc tttttcccgc
     3061 gtcgccaggc attacggcgc tcacgtacca gcgctgtttt gcgcacaata cgattgcccg
     3121 gtgcatgcac aaagcagcca ttatccggcg cgaatccgtg cttaatttca agttcacggc
     3181 atgcacgact gagtttttcc tggctccacg gaaggcaatt gatatagccg gtttcagggt
     3241 ggacacgatt cactgcaaca tgtacatgca ggttatccgt gtctgtatga acagctgcca
     3301 catactgatg ttttgacagc tcaagggatt tcagggtgtg acgcacacag tcaaaaagct
     3361 gctcagaacg cggactttcg tgcgcaggcc aggagagtat atagtgaaaa acaggatctg
     3421 tatcatcttt ggaaaaatga gcaaggtctg ctgctttctg catttcttca gccgcagatt
     3481 caagagacaa acagttatga aagcaggtca caccatagaa attcacccac tgactgccgt
     3541 ctttcatcac atcaatcagg gaaacaaatt tttcattacg cagacgtgtt gcataatcaa
     3601 caagacgtct gaagcggttc cggtgcggaa tctcagcttt catttccgtg tctggcctga
     3661 gttcatcttc cggcacatca tcccgcactg aggtgtacgc aatcaggtct tcaaaagaag
     3721 actttccgtc gcgtcttttt ttcggaatga tggcattcat cagcgattat ctcagcgtcc
     3781 cattaaatct cttcgcagag tattagcaag ctctgtaata gcaacgagaa cctctgcata
     3841 ctctttatct cccgtgcgtt ttccttccac aaacagatgt ttctgcaggc gaccaagacg
     3901 catcagctct ttcaaaaaat cttcgtcaat gcgggcattt atctgttttc ccagcacagt
     3961 ttttcttatc agagttgaaa ctgtcagtcc gtttttttct gccttttctt taacaatctc
     4021 agcctcttct ggtgaaaaac gaacagcaat gaggacattc ttttgccgtt tctcactgcc
     4081 acttcgggtt ttcttctcac tcatggtctg ctccttaccg gggtgtcggg gctaagccct
     4141 gaccaggtgg taatcgtata gccgtgcgtg cgcggttata cgattacaca tcctgtcccg
     4201 tttttcaggc attatagcct gttatttttg tgcatcatag tcttatgcag acggcagagg
     4261 cggggcgcag ctctgccagg aggcagtttt tgatgataaa atcagacttc aacatgaaac
     4321 ggagaaaaag tgccaatggc ttctaaaaaa ttttactcag atgatgacat ccagctggca
     4381 aaagctgctt tatcagaatt gccagatctc accactcaac ggaaaacatt gcgtgatttt
     4441 cttgatgcca ttcgtgatga cattattgtc ctggtaagga caaagggtta tacactggca
     4501 gatgttcgtg acacgctcaa aaatgcaggg tatgaagtag gagagaaagc acttcgtgac
     4561 attattcggg aagcagaatc gaaaaaaaca tcccgccgtt cttcctctaa aacagcacct
     4621 aaaaagacag attcaggcag gaaagatggc atcgatatga acaacaattg atgtgacaat
     4681 gtcacatcat gttctgagat tatgatgatt acagaaaaaa aagccccggt ataaatacca
     4741 gggcttttct gtttaatcag actcgtggcg gcaatgcacc aggataatgc cgtgcaataa
     4801 tatcattcag tttttcaaca aggtcaggac gcttaaagac aatgtgtacg gtccctttct
     4861 ggtagccacg caccgtaaaa tattcccctt ctaacagctt accgacgttg tcgccgttcc
     4921 cgagaaattc accatactgc gccccgatgc tgctccggaa gtcaggaacg ttgcgcccct
     4981 ccagcagata aaaaggacgt accaggtcat ccagtgcctc ccgtccgctg cagtccagcg
     5041 tcacacgtcc gttactccag cggtacagca tgttttcaat aatgattctc ttgcccagac
     5101 ggcagggatt attggttctg taatcccatg acaatttccg gtagacatca atcagtccct
     5161 gttcaaatgt ctgcatcttg ctggcattca gatggcggaa tgttgccagt acattgtcca
     5221 gggtgatttc aggacaggtg tcgctcatca gctggctgtt ccactcatca cgctggcatg
     5281 aactcatgaa ggtgtacatg cccgtatctg tcatcaggcg gtcccagatt ttccggtcgg
     5341 caagacgggt gatattcctt ttcgcttctt ccggtttatc tatgcgacga acggaatcag
     5401 tcacgcagtc ttcaaacccg taaagaaaaa tatcacctga cgcatcgagc agaatcttgc
     5461 gcgctgtcgc caggcattcc atcgcctgca tgaacagtgc aataccttcg ttacgctgcg
     5521 cgatgatgcg gtcgatagcc acagacggaa taacatctgt cgtgccggca gtattcaggt
     5581 cagcagtggg taaagtctga ttcatcacat tgtctcctca tcgtcacgac tgccgggtca
     5641 gccaggtcat ttcccggagg agcgaagctc cttctgccgt ccgggtcttc ggggatttcc
     5701 ccgcagtgga cctttccgac gggggacgct caccacgtca cggggcagtt gtccgggggc
     5761 agacgcaaaa gtttttgacg cctgcggcaa aaagttttgt ggctggtaca tctctcccct
     5821 ggacgactga aacgggatgt ggtcatgtat gtacccccgg aaagtccgtc tgcggagaac
     5881 aggccgcagt gcggcggcag acaggcagag acagctgcgc catcaggcgc agtggcctta
     5941 cacaccgtaa ggtgtgcagg gagcgccgca tacggcgcga ctgaagaaaa acgacggcac
     6001 aacaatggca tgtggtggtc actggcacac cactcccgca caggtgaaaa cagagaaaac
     6061 cgcacgacat ttacaagcaa aaaagtgaaa tttcttcact tttttacact tatttatcgc
     6121 gctgatttgg caattgtgat gataaaataa agtgaagttt tttcactttt tcaaaggtgt
     6181 tttatgaccg gctgggaact gcgaatctgg cgaaaaagca tgctctggtc acgcgaaaaa
     6241 gcggccagag aatttggcgt gacgcagaga acatggcacg cctgggaaaa tgcggaacag
     6301 gtggacgtca ccgtctggag gaccacccag gcactttcag tccgtgacct gctgcctcac
     6361 atgcagggga tgcgtaaggc agatatcatc aggcgactgg aaaatgaact gggggaaacg
     6421 gcagaggatg tgtgagcgtg attcacagcc tggctgaatg ccaggctact gctcatcagg
     6481 aaacagcttt ttcagttctt caccggaaag tccggttgcc tcctgaatcc gttccggcgt
     6541 ccagcccata tcccgcagcc ggcaggctat ttcccgcgcc acttcaagtg cgcctttttg
     6601 aataagacgt tctgcaatcg tcatgctgtt tgctctgctc ctgctgacaa aaaagcccgg
     6661 cattttgccg ggctgtgatg tttactcagt gagtgatttt cttcagttcg tcatcggaaa
     6721 gtccggtagc ctgcttaatc tgctccggcg tcatccccat tttctgaagc tggcacgcaa
     6781 tagccagggc tttttcaagc gctccttcca tgcgtccttc ctggcgacct ttttgctcaa
     6841 gacgttctgc gatagtcatc agtttatcct catgctgcgg cagacgctca gccagcgccc
     6901 gcataaattc cggtgataca gagtcccctg cgttaacgat gtaatgaatc agaacacgca
     6961 cctgctcgtc tgaaatttcc accatgacct gcgggagttt gtccagcagc tccatcatgt
     7021 cacgatggcg gatatgcttc atcagcagcg tcagggcggc catccggcga tggttcatga
     7081 tttcattgtc atcaacgaca gtgatatcaa ccagcggaaa cggctttgtg tatattttag
     7141 ccgcaagcgc cggattctca aaacagtcca gccagttcat gctgtacgga tacgggctgc
     7201 gctcaccatg atagaacagc accggaatca ccagtggcaa cgttttgtgc tgctccagat
     7261 gtctctgcat cgccgccaca ctgtaacgta acatgcggaa aggcatgtac agattttcgg
     7321 tgctctggtg ctcgatgagc gtataaacat agccatcttc acccccggtg gttttcacgc
     7381 tccagaggat atcgctggcg tactgatgca ggtccggctc aacaaaggtg gcgggttcca
     7441 gcttcagcgt ggacaggtcg cacaactgcc ggtactccgc cggaagatgc agttccagaa
     7501 aatctctggc gacgtcggga ttcgccagga acgaccggaa cgcggcatca tgcggcgtgg
     7561 gcgttgtggt gtttttcttc ttactcacgg ttcctcctgg tgagatgtct gaacgtatcc
     7621 tgccgcctgc acaggcagac ggcaagggtt aatcagtggc agacccgctc aatttcaagc
     7681 gcgcggagca ggtggcccca ttcgctgact ttcatagcgg ggatacgcac ccgcttctgc
     7741 tcactgcagc gtctggcgat atggccgctc atcgccagga cgtccgggtt aatctcatcc
     7801 tgtgccagct cctgacgtct gaagcgggta tggcggtgca ggggtttatt ctcccgcagg
     7861 cactcttcac gcagacgctt tacatactcg gcggttgcga agataattga tggtctcatg
     7921 gcataatctc catagatagt gattgagatg ccggacttcc tggcggggaa gcgccggcgg
     7981 agagaggcag ggtttatcgc cctgcctttt tttcatttcg gacagcgacg cagtgccagc
     8041 gcgagcgctt tcatcttcca gccttccggc gtgcggtaca tcaccttcca gtagccgccg
     8101 atgaacacat aatggaagcg cacctcttca gtgctttcag tggcaatagc ctccatgaaa
     8161 gaacggtact cacgcgcctc actgtccatc cggaaaccac tgtcacgtcc gtaataagcc
     8221 gtcacaccgt ccaccgggtt ctgaagcgta tgcccggcgg gtttgtcgca acggggaccg
     8281 aggcaacgga tatcaccgtg ttcaaccagg gcttcggcct gctgaccggt ggcatagtgg
     8341 gtatgcagga tgcgaccgtt ccaggtcggc tcaccgtgca ggtggcagta aacggtacgg
     8401 aacaccccgt cgcggcaaaa aacggaaatc gtgctgctga cagtcatgaa aacgtctcct
     8461 tctgctaaat caggtcgccg ggccgaccgg ttgctccccg gaggggcgaa agccccttct
     8521 gccgtccact tcttcggggt ttctccccgc agtggacctt tccgatgggg cgtccgtcac
     8581 cacgtcacgg ggcagctgtc cgggggcgga cgcaaaattt tttgccgggt ttatggctaa
     8641 aaattttgtg gacggtatat cttcccctgg actgctgaaa cgggatgtgg ttactccagc
     8701 ccccggaaag tccacagcgg gaaacaggcc gcaggggtgg cggcggaagg gttgactgcg
     8761 ccatcaggcg cagtggcctt acacaccgtc aggtgtgcag ggagcgccgt ttccggcgcg
     8821 acaggggcgg cgcgaccgga acggtcgctg caagcctggt ttttcagaca tcggaccgta
     8881 gcggcagccc gaaaccgcgc cagcgggttc ggcggagact ggcgggtgcg ccagcagctg
     8941 cctgtcggag tcgggcctga cgaccggatg ccgccaggca gccgggagcc gaaggtgtcc
     9001 ccgcctccgg aaatcagaac aacagaaacg acataacaga aaaatgcctc acaccatgac
     9061 gggcaggaag ggcagcgccg caggcgcggc cctcctgcat ttcaggcagg cgtaagcctg
     9121 ccagggtttt ccggcggcct ggcaggccat acccccgccg gagcggggga gtaaatcacc
     9181 gacggaacac atgaccgtca ataaaggtga aggagtccag gaataaatcc cgcgcatacg
     9241 cctcatagtc aaaatagagc gccaccgtct ccggcacgtc agccagcagg ccggtatcac
     9301 tggcgaactc aaccgcaaaa gcctcctcac tgtcagcctc gccccaccag gcatcgcgga
     9361 aggtgtcaaa atccgtctca ccggtatcct ccacccagag acgataagcc tcttcgcagc
     9421 cttcatcccg tgcccggcgg aagccttcaa cccaggccca gttgatatgg cattcagagg
     9481 ccatattccc cgggaatccc tcataatctt gaaacatcag ttcaggatcg gcttcatcct
     9541 ggtgaagagc acggcaggcg gcgaaaaagt cgcgctcatc atcaaacgtg gtcaggtcaa
     9601 accagcgtcc ggcgatgctt ccacagttgt atttgtgcca ggttccaacg tatacagcag
     9661 gtgcaacaac agacattccc gaat
//
LOCUS       DAZFZC010000148         8975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFZC010000148 DAZFZC010000000
VERSION     DAZFZC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.150"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(493..756)
                     /gene="rpsT"
                     /locus_tag="ACHKXP_004553"
     CDS             complement(493..756)
                     /gene="rpsT"
                     /locus_tag="ACHKXP_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO7649409.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            859..1077
                     /locus_tag="ACHKXP_004554"
     CDS             859..1077
                     /locus_tag="ACHKXP_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO7649410.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            1085..2026
                     /gene="ribF"
                     /locus_tag="ACHKXP_004555"
     CDS             1085..2026
                     /gene="ribF"
                     /locus_tag="ACHKXP_004555"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO7649411.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            2069..4885
                     /gene="ileS"
                     /locus_tag="ACHKXP_004556"
     CDS             2069..4885
                     /gene="ileS"
                     /locus_tag="ACHKXP_004556"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO7649412.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4885..5379
                     /gene="lspA"
                     /locus_tag="ACHKXP_004557"
     CDS             4885..5379
                     /gene="lspA"
                     /locus_tag="ACHKXP_004557"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO7649413.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5504..5953
                     /gene="fkpB"
                     /locus_tag="ACHKXP_004558"
     CDS             5504..5953
                     /gene="fkpB"
                     /locus_tag="ACHKXP_004558"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7649414.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5955..6905
                     /gene="ispH"
                     /locus_tag="ACHKXP_004559"
                     /gene_synonym="lytB"
     CDS             5955..6905
                     /gene="ispH"
                     /locus_tag="ACHKXP_004559"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO7649415.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6971..7885
                     /gene="rihC"
                     /locus_tag="ACHKXP_004560"
     CDS             6971..7885
                     /gene="rihC"
                     /locus_tag="ACHKXP_004560"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO7649416.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPHIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            8052..8873
                     /gene="dapB"
                     /locus_tag="ACHKXP_004561"
     CDS             8052..8873
                     /gene="dapB"
                     /locus_tag="ACHKXP_004561"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO7649417.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAIYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         2071 a         2213 c         2512 g         2179 t
ORIGIN      
        1 atttgtcatt atcagtcctt gaagttgatg aatttttatt aatttaattt atgagtgaat
       61 gtgactacta tcaaaaatcg ctcattaatt ttatgaagta caatataaat aggtatttaa
      121 cctgtgttga ttgttgttaa ttatttacaa gatctaataa ggagatattt atgaatggtt
      181 aaagtgtata ggaatttaat tcaaaaatag agtggtctta agtttaatac ctcaatgtgt
      241 atcacaatat ccacattctt tgtaggggag cctggagatt gagtagatat tcttgctcag
      301 aatgtatcag ccgatggttc tacgattctt aagccacgaa gagttcagat agtataacgg
      361 catgtctgca agaacctgca ttttttgact atctggcaac cggcagtgtg ttctctcacg
      421 catcacaaaa gcagcaggca taaaaaaacc cgcttgcgcg ggctttttca caaagcttca
      481 gcaaattggc gattaagcca gtttgttgat ctgtgcagtc aggttagcct tatgacgtgc
      541 agctttgttt ttgtggatca gacctttagc agcctgacgg tccacgatcg gttgcatttc
      601 gttaaatgct ttctgtgcag cagctttgtc gccagcttcg atagctgcgt atactttctt
      661 gatgaaagta cgcatcatag agcgacggct tgcgttgtgc ttacgagcct tttcagactg
      721 aatggcgcgc ttcttagctg atttgatatt agccaaggtc caactcccaa atgtgttcta
      781 tatggacaat tcaaaggccg aggaatatgc ccttttagcc ttcttttgtc aatggatttg
      841 tgcaaataag cgccgttaat gtgccggcac tcgttacgta gtgatggcgc aggattctac
      901 cagcttgcgg ggtgtgaata cagcttttcc gcgataaaaa ttgcagcagg cggtcacttt
      961 cttcccgtga tttgcgccat ggcaatgaaa agccacttct ttctgatttc ggtactcaat
     1021 cgccggttaa ccttgaccgc tgtacaaggt atactcggac gattttcact gttttgagcc
     1081 agacatgaag ctgatacgcg gcatacataa tctcagccag gccccgcaag aagggtgtgt
     1141 gctgactatt ggtaatttcg acggcgtgca tcgcggtcat cgcgcgctgt tacagggctt
     1201 gcaggaagaa gggcgcaagc gcaacttacc ggtgatggtg atgctttttg aacctcaacc
     1261 actggaactg tttgctaccg ataaagcccc ggcaagactg acccggctgc gggaaaaact
     1321 gcgttacctt gcagagtgtg gcgttgatta cgtgctgtgc gtgcgtttcg acaggcgttt
     1381 cgcggcgtta accgcgcaaa atttcatcag cgatcttctg gtgaagcatt tgcgcgtaaa
     1441 atttcttgcc gtaggtgatg atttccgctt tggcgctggt cgtgaaggcg atttcttgtt
     1501 attacagaaa gctggcatgg aatacggctt cgatatcacc agtacgcaaa ctttttgcga
     1561 aggtggcgtg cgcatcagca gcaccgccgt gcgtcaggcc cttgcggatg acaatctggc
     1621 tctggcagag agtttactgg ggcacccgtt tgccatctcc gggcgtgtag tccacggtga
     1681 tgaattaggg cgcactatag gtttcccgac ggcgaatgta ccgctgcgcc gtcaggtttc
     1741 cccggtgaaa ggggtttatg cggtagaagt gctgggcctc ggtgaaaagc cgttacccgg
     1801 cgtggcaaac atcggaacac gcccaacggt tgccggtatt cgccagcagc tggaagtgca
     1861 tttgttagat gttgcaatgg acctttacgg tcgccatata caagtagtgc tgcgtaaaaa
     1921 aatacgcaat gagcagcgat ttgcgtcgct ggacgaactg aaagcgcaga ttgcgcgtga
     1981 tgaattaacc gcccgcgaat tttttgggct aacaaaaccg gcttaagcct gttatgtaat
     2041 caaaccgaaa tacggaaccg agaatctgat gagtgactat aaatcaaccc tgaatttgcc
     2101 ggaaacaggg ttcccgatgc gtggcgatct cgccaagcgc gaacccggaa tgctggcgcg
     2161 ttggactgat gatgatctgt acggcatcat ccgtgcggct aaaaaaggca aaaaaacctt
     2221 cattctgcat gatggccctc cttatgcgaa tggcagcatt catattggtc actcggttaa
     2281 caagattctg aaagacatta tcgtgaagtc caaagggctt tccggttatg actcgccgta
     2341 tgtgcctggc tgggactgcc acggtctgcc gatcgagctg aaagtcgagc aagaatacgg
     2401 taagccgggt gagaaattca ccgccgccga gttccgcgcc aagtgccgcg aatacgcggc
     2461 gacccaggtt gacggtcaac gcaaagactt tatccgtctg ggcgtgctgg gcgactggtc
     2521 gcacccgtac ctgaccatgg acttcaaaac tgaagccaac atcatccgcg cgctgggcaa
     2581 aatcatcggc aacggtcacc tgcacaaagg cgcgaagcca gttcactggt gcgttgactg
     2641 ccgttctgcg ctggcggaag cggaagttga gtattacgac aaaacttctc cgtccatcga
     2701 cgttgctttc caggcagtcg atcaggatgc actgaaagca aaatttgccg taagcaacgt
     2761 taacggccca atctcgctgg tgatctggac caccacgccg tggactctgc ctgcgaaccg
     2821 cgcaatctct attgcacctg atttcgacta tgcgctggtg cagatcgacg gtcaggccgt
     2881 gattctggcg aaagatctgg ttgaaagcgt aatgcagcgt atcggcgtga ccgattacac
     2941 cattctcggc acggtaaaag gtgcggagct tgagttgctg cgctttaccc atccgtttat
     3001 gggcttcgac gttccggcaa tcctcggcga tcacgttacc ctggatgccg gtaccggtgc
     3061 cgttcacacc gcgcctggcc acggcccgga cgactatgtg atcggtcaga aatacggcct
     3121 ggaaaccgct aacccggttg gcccggacgg cacttatctg ccgggcactt atccgacgct
     3181 ggatggcgtg aacgtcttca aagcgaacga catcgtcgtt gcgctgctgc aggaaaaagg
     3241 cgcgctgctg cacgttgaga aaatgcagca cagctatccg tgctgctggc gtcacaaaac
     3301 gccgatcatc ttccgcgcga cgccgcagtg gttcgtcagc atggatcaga aaggtctgcg
     3361 tgcgcagtca ctgaaagaga tcaaaggcgt gcagtggatc ccggactggg gccaggcgcg
     3421 tatcgagtcg atggttgcta accgtcctga ctggtgtatc tcccgtcagc gcacctgggg
     3481 tgtaccgatg tcactgttcg tgcacaaaga cacggaagag ctgcatccgc gtacccttga
     3541 actgatggaa gaagtggcaa aacgcgttga agtcgatggc atccaggcgt ggtgggatct
     3601 cgatgcgaaa gagatcctcg gcgacgaagc tgatcagtac gtgaaagtgc cggacacatt
     3661 ggatgtatgg tttgactccg gatctaccca ctcttctgtt gttgacgtgc gtccggaatt
     3721 tgccggtcac gcagcggaca tgtatctgga aggttctgac caacaccgcg gctggttcat
     3781 gtcttcccta atgatctcca ccgcgatgaa gggcaaagcg ccgtatcgtc aggtactgac
     3841 ccacggcttt accgtggatg gtcaggggcg caagatgtct aaatccatcg gcaacaccgt
     3901 ttcgccgcag gatgtgatga acaaactggg tgcggatatt ctgcgtctgt gggtggcatc
     3961 aactgactac accggtgaaa tggccgtttc tgacgagatc ctgaaacgtg ctgccgacag
     4021 ctatcgtcgt atccgtaaca ccgcgcgctt cctgctggca aacctgaacg gttttgatcc
     4081 agcaaaagat atggtgaaac cggaagagat ggtggtactg gatcgctggg ccgtaggttg
     4141 tgcgaaagcg gcacaggaag acatcctcaa ggcatacgaa gcatacgatt tccacgaagt
     4201 ggtacagcgt ctgatgcgct tctgctccgt tgagatgggt tccttctacc tcgacatcat
     4261 caaagaccgt cagtacaccg ccaaagcgga cagtgtggcg cgtcgtagct gccagactgc
     4321 gctgtatcac atcgcagaag cgctggtgcg ctggatggca ccaatcctct ccttcaccgc
     4381 tgatgaagtg tggggctacc tgccgggcga acgtgaaaaa tacgtcttca ccggcgagtg
     4441 gtacgaaggc ctgtttggtc tggcagacag tgaagcgatg aacgatgcgt tctgggacga
     4501 gctgttgaaa gtgcgtggcg aagtgaacaa agtcattgag caagcgcgtg ccgacaagaa
     4561 agtgggcggc tcgctggaag cggcagtaac cttgtatgca gaaccggaac tggcggcgaa
     4621 actgaccgcg ctgggcgatg aattacgatt tgtcctgttg acctccggcg ctaccgttgc
     4681 agactataac gacgcacctg ctgatgctca gcagagcgaa gtactcaaag gactgaaagt
     4741 cgcgttgagt aaagccgaag gtgagaagtg cccacgctgc tggcactaca cccaggatgt
     4801 cggcaaggtg gcggaacacg cagaaatctg cggccgctgt gtcagcaacg tcgccggtga
     4861 cggtgaaaaa cgtaagtttg cctgatgagt caatcgatct gttcaacagg gctacgctgg
     4921 ctgtggctgg tggtagtcgt gctgattatc gatctgggca gcaaatacct gatcctccag
     4981 aactttgctc tgggggatac ggtcccgctg ttcccgtcgc ttaatctgca ttatgcgcgt
     5041 aactatggcg cggcgtttag tttccttgcc gatagcggcg gctggcagcg ttggttcttt
     5101 gccggtattg cgattggtat tagcgtgatc ctggcagtga tgatgtatcg ctcgaaggcc
     5161 acgcagaagc taaacaatat cgcttacgcg ctgattattg gcggcgcgct gggcaacctg
     5221 ttcgaccgcc tgtggcacgg cttcgttgtc gatatgatcg acttctacgt cggcgactgg
     5281 cacttcgcca ccttcaacct tgccgatact gccatctgtg tcggtgcggc actgattgtg
     5341 ctggaaggtt ttttgccttc tagagcgaaa aaacaataat aaaccctgcc ggatgcgatg
     5401 ctgacgcatc ttatccggcc tacagattgc tgcgaaatcg taggccggat aaggcgttta
     5461 cgccgcatcc ggcaaaaatc cttaaatata agagcaaacc tgcatgtctg aatctgtaca
     5521 gagcaatagc gccgtcctgg tgcacttcac gctgaaactc gatgatggca ccaccgctga
     5581 gtctacccgc aacaacggta aaccggcgct gttccgcctg ggcgatgctt ctctttctga
     5641 agggctggag caacacctgc tggggctgaa agtgggcgat aaaaccacct tctcgctgga
     5701 gcccgatgcg gcgtttggcg tgccgtcacc ggacctgatt cagtacttct cccgccgtga
     5761 atttatggat gcaggcgagc cagaaattgg cgcaattatg ctttttaccg caatggatgg
     5821 cagtgagatg cctggcgtga tccgcgaaat taacggcgac tccattaccg ttgatttcaa
     5881 ccatccgctg gccgggcaga ccgttcattt tgatattgaa gtgctggaaa tcgatccggc
     5941 actggaggcg taacatgcag atcctgttgg ccaacccgcg tggtttttgt gccggggtag
     6001 accgcgctat cagcattgtt gaaaacgcgc tggccattta cggcgcaccg atatatgtcc
     6061 gtcacgaagt ggtgcataac cgctacgtgg tcgatagcct gcgcgagcgt ggggctatct
     6121 ttattgagca gattagcgaa gtaccggacg gcgcgatcct gattttctcc gcacacggtg
     6181 tttctcaggc ggtacgtaac gaagcaaaaa gccgcgattt gacggtgttt gatgccacct
     6241 gtccgctggt gaccaaagtg catatggaag tcgcccgcgc cagccgtcgt ggcgaagagt
     6301 cgattctcat cggccacgcc gggcacccgg aagtggaagg tacgatgggc cagtacagta
     6361 acccggaagg gggaatgtat ctggtcgaat cgccggacga tgtgtggaaa ctgacggtca
     6421 aaaacgaaga gaagctctcc tttatgaccc agaccacgct gtcggtggat gacacgtctg
     6481 atgtgatcga cgcgctgcgt aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca
     6541 tctgctacgc cacgactaac cgtcaggaag cggtacgcgc cctggcagaa caggcggaag
     6601 ttgtgctggt ggtcggttcg aaaaactcct ccaactccaa ccgtctggcg gagctggccc
     6661 agcgtatggg caaacgcgcg tttttgattg acgatgcgaa agacatccag gaagagtggg
     6721 tgaaagaggt taaatgcgtc ggcgtgactg cgggcgcatc ggctccggat attctggtgc
     6781 agaatgtggt ggcacgtttg cagcagctgg gcggtggtga agccattccg ctggaaggcc
     6841 gtgaagaaaa cattgttttc gaagtgccga aagagctgcg tgtcgatatt cgtgaagtcg
     6901 attaagtcat tagcagccta agttatgcga aaatgccggt cttgttaccg gcatttttta
     6961 tggagaaaac atgcgtttac ctatcttcct cgatactgac cccggcattg acgatgccgt
     7021 cgccattgcc gccgcgattt ttgcacccga actcgacctg caactgatga ccaccgtcgc
     7081 gggtaatgtc tcggttgaga agactacccg caatgccctg caactgctgc atttctggaa
     7141 tgcggagatt ccgctcgccc aaggggccgc tgtgccactg gtacgcgcac cgcgtgatgc
     7201 ggcatctgtg cacggcgaat cgggaatggc tggctacgac tttgttgagc acaaccgaaa
     7261 gccgctcggg ataccggcgt ttctggcgat tcgggatgcc ctgatgcgtg caccagagcc
     7321 tgttactctg gtagccatcg gcccgttaac caatattgcg ctgttacttt cacaatgccc
     7381 ggaatgcaaa ccgcatattc gccgtctggt gatcatgggt ggttctgccg gtcgcggcaa
     7441 ctgcacacca aacgccgagt ttaatatcgc tgccgatcca gaagcggctg cctgtgtctt
     7501 ccgtagcgga atcgaaattg tcatgtgcgg tctggatgtc accaatcagg caatattaac
     7561 ccctgactat ctcgctactt tgccggagtt gaatcgcacc ggaaaaatgc tccacgccct
     7621 gtttagccac taccgtagcg gcagtatgca aagcggtctg cgaatgcacg atctctgcgc
     7681 catcgcctgg ctggtgcgcc cggacttatt caccctcaag ccgtgttttg tcgcggtaga
     7741 aactcaaggc gaatttacct caggcacgac ggtggttgat atcgacggtt gcctgggcaa
     7801 accagccaat gtacaggtgg cattggatct gaatgtaaaa ggcttccagc agtgggtagc
     7861 tgaggtgctg gctctggtgc cgtaacctgt cacatgttat cggcatgcag tcattcatcg
     7921 actcatgcct ttcactgata tccctccctg tttatcatta atttctaatt atcagcgttt
     7981 ttggctggcg gcgtagcgat gcgctggtta ctctgaaaac ggtctatgca aattaacaaa
     8041 agagaatagc tatgcatgat gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta
     8101 tgggccgcca gttgattcag gcggcgctgg cattagaggg cgtgcagttg ggcgctgcgc
     8161 tggagcgtga aggatcttct ttactgggca gcgacgccgg tgagctggcc ggagccggga
     8221 aaacaggcgt taccgtgcaa agcagcctcg atgcggtaaa agatgatttt gatgtgttta
     8281 tcgattttac ccgtccggaa ggtacgctga accatctcgc tttttgtcgc cagcatggca
     8341 aagggatggt gatcggcact acggggtttg acgaagccgg taaacaagca attcgtgacg
     8401 ccgctgccga tattgcgatt gtctttgctg ccaattttag cgttggcgtt aacgtcatgc
     8461 ttaagctgct ggagaaagca gccaaagtga tgggtgacta caccgatatc gaaattattg
     8521 aagcacatca tagacataaa gttgatgcgc cgtcaggcac cgcactggca atgggagagg
     8581 cgatcgccca cgcccttgat aaagatctga aagattgcgc gatctacagt cgtgaaggcc
     8641 ataccggtga acgtgtgcct ggtaccattg gttttgccac cgtgcgtgca ggtgacatcg
     8701 ttggtgaaca taccgcgatg tttgccgata ttggcgagcg tctggagatc acccataagg
     8761 cgtccagccg tatgacattt gctaacggcg cggtaagatc ggctttgtgg ttgagtggta
     8821 aggaaagcgg tctttttgat atgcgagatg tacttgatct caataatttg taaccacaaa
     8881 atatttgtta tggtgcaaaa ataatacatt taatttattg attataaagg gctttaattt
     8941 ttggcccttt tatttttggt attatgtttt taaat
//
LOCUS       DAZFZC010000149         8951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFZC010000149 DAZFZC010000000
VERSION     DAZFZC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.151"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(33..272)
                     /locus_tag="ACHKXP_004562"
                     /pseudo
     CDS             complement(33..272)
                     /locus_tag="ACHKXP_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
     gene            376..1899
                     /gene="istA"
                     /locus_tag="ACHKXP_004563"
     CDS             376..1899
                     /gene="istA"
                     /locus_tag="ACHKXP_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HFO7649418.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            1886..2671
                     /gene="istB"
                     /locus_tag="ACHKXP_004564"
     CDS             1886..2671
                     /gene="istB"
                     /locus_tag="ACHKXP_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HFO7649419.1"
                     /translation="MMQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSL
                     IKAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNV
                     VLIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLL
                     YADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTT
                     ALLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            2753..4291
                     /locus_tag="ACHKXP_004565"
     CDS             2753..4291
                     /locus_tag="ACHKXP_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7649420.1"
                     /translation="MYSYADRIRAVELYIKLGLRVRATIRQLGYPTKNALKGWYQHYL
                     KHQDLPASQVPRAPKYSLQQRQVAVAHYLAHDRCIAATMRVLGYPGRGTLTAWVRQDC
                     PEASQSILGRSWPVTKPDALMYEGVVQLCTRQSSAQEIADKLGVCRGTLYNWKNQLLG
                     PCAPASMKHSPKRSPVLDAAALRCQVESLRQDVRRLKIERELLKQAHEILKNGADIDL
                     HRLANKDKAVLVEALHGQYELPELLCHVGLARSSYFYHRARLKLADKYLDVRRSITDI
                     FDSNYRCYGYRRVQASLLKECTGISEKVVRRLMKQEGLIVVKPKRRRYNSYLGEIGAA
                     PQNLINRDFHAAAPNEKWLTDITEFQIPSGKVYLSPVIDCFDGLVVSWSIGTHPNANL
                     VNTMLDAAIDAVQGSESRPMIHSDRGAHYRWPGWLSRVHDAKLVRSMSRKGCSPDNAA
                     CEGFFGRLKMELFYPGNWQSTTIEQFIEAVDTYIRWYNEKRIKVSLGRLSPVEYRQKL
                     GLTT"
     gene            complement(4344..4577)
                     /locus_tag="ACHKXP_004566"
     CDS             complement(4344..4577)
                     /locus_tag="ACHKXP_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649421.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEC"
     gene            complement(4705..5544)
                     /gene="sul1"
                     /locus_tag="ACHKXP_004567"
     CDS             complement(4705..5544)
                     /gene="sul1"
                     /locus_tag="ACHKXP_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO7649422.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(5538..5885)
                     /locus_tag="ACHKXP_004568"
     CDS             complement(5538..5885)
                     /locus_tag="ACHKXP_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO7649423.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            6106..7149
                     /locus_tag="ACHKXP_004569"
     CDS             6106..7149
                     /locus_tag="ACHKXP_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO7649424.1"
                     /translation="MKRIAVDLAKSVYQVAESVRSGQVVQRKRLNREAFRRYIQEQAE
                     PVEWVMEACGTAHYWGRVAQALGHQVRLLHPRYVRPYRRRNKTDRNDCDAMLEATRCT
                     DIHPVPVKSHDQQQLQQLHGLRETWKKSRTQRINLLRGLLREAGIEAPASTAAFIRAA
                     SERVDQPELAPLSRLLHIVLAEINLYEQCMAECEQQLKRWHADDDIVRKLDEVSGIGL
                     LTASALTAAVGRPERFASGRQLSAWLGMTPREFSSGERRKLGHISRQGNVYVRTLLIH
                     GSRAALLAAQRCQARTPEKLTQLQRWAVATAARIGHNKAAVALANKLVRICWAVWCHE
                     RRFNGNWQSTKPA"
     gene            complement(7391..8220)
                     /gene="aadA1"
                     /locus_tag="ACHKXP_004570"
                     /pseudo
     CDS             complement(7391..8220)
                     /gene="aadA1"
                     /locus_tag="ACHKXP_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(8278..8811)
                     /gene="aadB"
                     /locus_tag="ACHKXP_004571"
     CDS             complement(8278..8811)
                     /gene="aadB"
                     /locus_tag="ACHKXP_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HFO7649425.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
BASE COUNT         2096 a         2486 c         2490 g         1879 t
ORIGIN      
        1 ggggcacctc agaaaacgga aaataaagca cgctaaggca tagctgacct tgccaggcct
       61 gcttcgccct gtagtgacgc gatcaacggg caggaaacat tcccctttcg tgcatggcag
      121 gcgcacacga gttcagacag cacggtttcc atgcgcgcca agtcggccat cttctcgcgc
      181 acgtccttga gcttgtgttc ggccaggctg ctggcctcct cgcagtgggt gccatcgtcg
      241 agccgcaaca gctcggcaat ctcgtccatg ttgagttgca tctaaaattg acccacttag
      301 ggtaaagatt tgcgtcgaaa tttgacccac gtatgacact gtttcccgtc tggatatggc
      361 gggagaaatc aaggagtgat aaacgtggcg atattgagcg caattcgacg ctggcatttt
      421 cgcgatggtg cgtcgattcg ggaaatagcc cgacgaagcg gcctgtccag gaacaccgtt
      481 cgcaagtatt tgcaaagcaa ggtggttgaa ccgcagtacc cagcgcgaga cagcgttggc
      541 aagttaagtc cttttgagcc caagttaagg cagtggctct ccaccgagca caaaaagaca
      601 aagaagctgc gcagaaacct gcgcagcatg taccgggatt tggtcgcttt gggctttacc
      661 gggtcttatg accgagtgtg tgcctttgcc cgacagtgga aagattccga acagttcaag
      721 gcgcaaacct cgggcaaggg ttgtttcatc cccttgcgct ttgcttgtgg cgaagccttc
      781 caattcgatt ggagtgagga ctttgcccgc atagcgggca aacaggtcaa acttcagatt
      841 gcccagttta agttggccca cagccgggcc tttgtgcttc gggcttacta ccagcaaaaa
      901 catgaaatgc tgtttgatgc ccactggcat gcctttcaaa tcttcggtgg cattcccaag
      961 cgcggcatct acgacaacat gaagaccgct gtggattcgg tggggcgtgg caaagagcgc
     1021 agggtcaatc agcggttcac tgccatggtc agccactacc tgtttgatgc gcagttctgt
     1081 aatccagcat cgggttggga gaaaggccag attgagaaga acgtgcagga ttcccgccaa
     1141 cgcctgtggc aaggggcacc agactttcaa agccttgctg atttgaatgt gtggcttgag
     1201 catcgctgca aagcgctgtg gtctgagctg cgccaccccg aattggacca aaccgtgcaa
     1261 gaggcctttg ccgatgaaca aggcgagttg atggcgctac ccaatgcctt tgatgcattc
     1321 gtggagcaaa ccaagcgagt cacttcaacc tgccttgttc accacgaggg caatcgctac
     1381 agcgttcctg ccagttacgc caacagggcc atcagccttc ggatttatgc agacaagctg
     1441 gtgatggctg ccgaaggcca acacattgcc gagcatccaa gattgtttgg cagtggccac
     1501 gctcggcgtg gccacacaca atacgactgg caccattact tgtctgtgct tcagaagaaa
     1561 cctggggcgt tgcgcaatgg tgcgccattt gctgaattgc cacccgcgtt caagaagctt
     1621 caatccatct tgctgcaacg ccccggcggt gaccgtgaca tggtggaaat tctggccctt
     1681 gtattgcacc acgatgaagg tgcggtactc agtgctgtgg aattggcatt ggagtgtggc
     1741 aagccatcga aggagcatgt gcttaatctg ttgggacgtt tgaccgaaga acctccaccc
     1801 aaaccgattc caattcccaa ggggttaagg ctgacattgg aaccacaggc caacgtgaac
     1861 cgctatgaca gtttaaggag agcccatgat gcagcatgaa ggccatgtga gaatcctcaa
     1921 atccttgaaa ctctttggca tggcacacgc cattgaggag ttgggcaatc agaattcacc
     1981 agcatttaat caagccttgc ccatgctgga cagcttgatt aaagctgaag tggcagagcg
     2041 tgaagtacgt tcggtgaact atcaattgcg ggtggccaag ttccccgtgt atcgggactt
     2101 ggtgggcttt gacttcagtc aaagcctggt taatgaggcc acggtcaaac aattgcaccg
     2161 gtgcgacttc atggaacaag cccagaacgt ggtgctgatt ggtgggccag gcacaggcaa
     2221 gactcacctg gccacagcca ttggtacaca agcagtgatg cacttgaacc gacgggtgcg
     2281 tttcttctcc accgtggatt tggtcaatgc actggagcaa gagaaatcat ctgggcgtca
     2341 gggacaaatc gcaaaccgtc tgttgtatgc cgatttggtg attctggatg agctgggata
     2401 tttgcctttt agccaaaccg gtggggcact gctgtttcac ctgctctcaa agctgtacga
     2461 aaaaaccagc gtgatactga ccaccaactt gagcttctcg gaatggagcc gagtgtttgg
     2521 cgatgaaaag atgacaacag cgttgttgga ccgactaacc caccactgcc acatcctgga
     2581 aaccggcaat gaaagttacc gcttcaaaca cagttcaact cagaataagc aggaggaaaa
     2641 acagacccgc aaactgaaaa tcgagacata attctgacaa caaggggtgg gtcaaaattc
     2701 aatgcaaatc ccggggatgc ggacaaaaac ttggactacc aggaggtagt tcatgtactc
     2761 atacgcagac aggattcgtg cagttgagct ttacatcaag cttggcttac gtgttagagc
     2821 cacgattcgt cagttgggat acccaaccaa gaacgcattg aaaggctggt atcagcacta
     2881 cctgaagcat caggatttgc cagcaagcca agtgccaaga gcacccaaat attcgctgca
     2941 gcaaagacag gttgcggttg ctcattacct cgcccacgat cgctgtattg ctgccacgat
     3001 gagggttttg ggttacccgg gtcgaggaac gttgactgcg tgggttcggc aagactgccc
     3061 cgaggccagc caatcgatac taggcaggtc ttggccggtt acaaagccgg atgctttgat
     3121 gtatgaaggc gtggtgcaac tgtgtacccg ccaatcaagc gcacaggaga ttgcggacaa
     3181 gttaggggta tgccgaggaa ccttgtacaa ctggaaaaat cagttgcttg gcccttgcgc
     3241 ccccgcctcg atgaaacaca gtccaaagcg atcgccagtg ttggatgcag cagcgctcag
     3301 gtgccaagtt gaatcattgc gccaggatgt tcggcggctg aagatagagc gtgaacttct
     3361 caagcaggcc catgaaatct taaaaaatgg ggctgacatt gatctgcata ggctggcaaa
     3421 caaagataag gctgtgctgg ttgaagcgct gcacgggcag tatgaattgc cagaactgct
     3481 ttgccatgtt ggccttgcta gaagctcgta tttttaccat cgtgcccggc tgaagctggc
     3541 tgacaaatac ttggatgtac gccgaagcat caccgatatc ttcgacagca actaccgttg
     3601 ctatggatat cgccgagtcc aggcatccct gctcaaggag tgcacgggca tctcagagaa
     3661 ggtggttcgt cggctaatga agcaggaggg gttgatcgtg gtcaagccca aacgccgcag
     3721 atataactca taccttggag agataggcgc tgccccgcaa aacctcatca atcgagactt
     3781 ccatgctgct gcgccgaatg agaagtggct taccgacatc actgaattcc agattccttc
     3841 cggcaaggtt tacctgtcgc cagtgattga ttgctttgac gggctggtgg tgagctggtc
     3901 cattgggact catcccaacg ccaatctcgt caacacaatg ctggatgcag ccattgatgc
     3961 tgttcaaggc agtgagagcc gccctatgat tcattctgat cgtggagctc actaccgctg
     4021 gccgggctgg ctctcgcggg tgcacgatgc aaagttggtt cgttcaatgt cccgcaaagg
     4081 gtgctcacca gacaacgcag catgcgaagg cttctttgga aggctgaaaa tggagttgtt
     4141 ctatcccggc aactggcaat caacgacgat tgagcagttc attgaagccg tcgatactta
     4201 catccgttgg tacaacgaaa agcgcatcaa ggtatctctg gggcgtttaa gtcctgtgga
     4261 atacaggcaa aaacttggcc taaccacata aacccagtcc aagtttttat ccgcaccccc
     4321 cccgggtcaa atttgggtgc aaatcaacac tcggccgaca aggtgcaaca gccgacgatg
     4381 ccgtcgctgc aactcgcgac taggagctcg gatctcagga cgaaggtctc cgcgaatgtc
     4441 cggtcgatcc gcgcgacgtc ccaggcgggc gttcccttgg cggacatcca cgccgcagcg
     4501 tcgtgcatca gccgcacaac ctcgtcgata tcacccgagc aggcgacccg aacgttcgga
     4561 ggctcctcgc tgtccattcg ctcccctggc gcggtatgaa ccgccgcctc atagtgcagt
     4621 ttgatcctga cgagcccagc atgtctgcgc ccaccttcgc ggaacctgac cagggtccgc
     4681 tagcgggcgg ccggaaggtg aatgctaggc atgatctaac cctcggtctc tggcgtcgcg
     4741 actgcgaaat ttcgcgaggg tttccgagaa ggtgattgcg cttcgcagat ctccaggcgc
     4801 gtgggtgcgg acgtagtcag cgccattgcc gatcgcgtga agttccgccg caaggctcgc
     4861 tggacccaga tcctttacag gaaggccaac ggtggcgccc aagaaggatt tccgcgacac
     4921 cgagaccaat agcggaagcc ccaacgccga cttcagcttt tgaaggttcg acagcacgtg
     4981 cagcgatgtt tccggtgcgg ggctcaagaa aaatcccatc cccggatcga ggatgagccg
     5041 gtcggcagcg accccgctcc gtcgcaaggc ggaaacccgc gcctcgaaga accgcacaat
     5101 ctcgtcgagc gcgtcttcgg gtcgaaggtg accggtgcgg gtggcgatgc catcccgctg
     5161 cgctgagtgc ataaccacca gcctgcagtc cgcctcagca atatcgggat agagcgcagg
     5221 gtcaggaaat ccttggatat cgttcaggta gcccacgccg cgcttgagcg catagcgctg
     5281 ggtttccggt tggaagctgt cgattgaaac acggtgcatc tgatcggaca gggcgtctaa
     5341 gagcggcgca atacgtctga tctcatcggc cggcgataca ggcctcgcgt ccggatggct
     5401 ggcggccggt ccgacatcca cgacgtctga tccgactcgc agcatttcga tcgccgcggt
     5461 gacagcgccg gcggggtcta gccgccggct ctcatcgaag aaggagtcct cggtgagatt
     5521 cagaatgccg aacaccgtca ccatggcgtc ggcctccgca gcgacttcca cgatggggat
     5581 cgggcgagca aaaaggcagc aattatgagc cccataccta caaagcccca cgcatcaagc
     5641 ttttgcccat gaagcaacca ggcaatggct gtaattatga cgacgccgag tcccgaccag
     5701 actgcataag caacaccgac agggatggat ttcagaacca gagaaagaaa ataaaatgcg
     5761 atgccataac cgattatgac aacggcggaa ggggcaagct tagtaaagcc ctcgctagat
     5821 tttaatgcgg atgttgcgat tacttcgcca actattgcga taacaagaaa aagccagcct
     5881 ttcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag
     5941 cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taacgcttga
     6001 gttaagccgc gccgcgaagc ggcgtcggct tgaatgctgt aatggactcc cccgcaccac
     6061 ccttatatac caggcatcgt ggtggctgaa attgggagag gcgatatgaa acgcattgca
     6121 gttgatctgg ccaagtccgt ttaccaagtt gccgagagtg tccgttccgg ccaggtggtg
     6181 cagcgcaagc ggctgaaccg agaggcgttc cggcgatata tacaggagca ggccgaaccg
     6241 gtcgagtggg tgatggaggc ctgtggcacg gcccactact ggggacgtgt agcgcaagcc
     6301 ctggggcatc aggtgaggct gctgcatccg cgctacgtgc ggccctatcg ccgccgcaac
     6361 aagaccgatc gcaatgactg cgacgccatg ctcgaagcga cgcgctgcac cgatattcat
     6421 cccgtgccgg tgaaaagcca cgaccagcaa cagctgcaac agttgcacgg gctgcgggaa
     6481 acctggaaga aaagccgcac ccagcggatc aacctgctgc gcggcctctt gcgtgaagct
     6541 ggtatcgagg cccccgcctc gaccgctgcc ttcatccgcg ccgccagcga gcgggttgac
     6601 cagcccgagc tcgcacccct gagccgtctg ctgcatatcg tcctggccga aatcaacctg
     6661 tatgaacagt gcatggccga gtgcgagcag cagctcaagc gctggcatgc cgacgatgac
     6721 atcgtacgca agctggatga ggtcagcggc attgggctgc tgaccgccag cgccctgaca
     6781 gccgccgttg gcaggcccga gcgctttgcc agtggtcgcc agctcagtgc ctggctgggc
     6841 atgaccccgc gcgagttcag cagtggcgag cgccgcaagc tcggccacat cagccgacag
     6901 ggcaacgtct atgtgcgtac cctgctaatc catggctcac gggcagcctt gctggctgca
     6961 caacgctgtc aggcacgcac cccggaaaag ctgacgcaac tgcaacgctg ggccgtggca
     7021 acggcggcgc gcatcggcca caacaaggca gcggtagccc tggccaacaa actggtgcgg
     7081 atctgctggg ccgtgtggtg ccatgagcgg cgcttcaatg gcaattggca aagcacaaag
     7141 cccgcctgac ggcgggagta atcagaacgg caagatggtt ttcaggtggt tgtggtgaac
     7201 agcagcactg tcggaacagg cgagtcctgc agcggaataa ggccgataac aatcatgatc
     7261 cgcgtgatcg attgaacgct tggcccccgc tgcgcgaact acagattggc cagggcggaa
     7321 aagcccacaa caggccggat atacgaatgc aaccgcgctg acgacaggtg caaaagcaat
     7381 gctttgctca ctacttgtgg ggagagtcca tatacgaatt gttagacatt atttgccgac
     7441 taccttggtg atctcgcctt tcacgtagtg aacaaattct tccaactgat ctgcgcggga
     7501 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     7561 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     7621 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     7681 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     7741 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     7801 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     7861 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     7921 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     7981 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     8041 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     8101 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     8161 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
     8221 gatgtttaac acctgagtta agccgcgccg cgaagcggcg tcggcttgga cgaattgtta
     8281 ggccgcatat cgcgacctga aagcggcacg caagacctca accttttccg ccccgagtga
     8341 ggtgcatgcg agcctgtagg actctatgtg ctttgtaggc cagtccactg gtggtacttc
     8401 atcggcatag taaaagtaat cccagatgat cgcctcccag ctgttacaac ggactggccg
     8461 cccggcgatg acgccctcag ccgcctctgg gcacgagccc tgcggagcct ccgcgatttc
     8521 atacgcttcg tctgcccacc aagcaggttc gcagtcaagt aactcatccc cgatctccgc
     8581 taagaatcca tagtccaact cctccatgac gcgcccgccg agcatttcaa ctattgcctc
     8641 gagctcgccg cgcctctcgc cgggaaacgt cagatcaata tcatcgtgct tgcgtgttac
     8701 acgccctagc cgtgcatcga tcgcccagcc cccaccgatc cagagcggca gatttcgctc
     8761 atctgccgca gctagaattt tgtgtatcaa tgtgacctgc gttgtgtcca tgcggcctat
     8821 ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     8881 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     8941 tagactgtac c
//
LOCUS       DAZFZC010000150         8926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFZC010000150 DAZFZC010000000
VERSION     DAZFZC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.152"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..99)
                     /gene="ykiE"
                     /locus_tag="ACHKXP_004572"
     CDS             complement(31..99)
                     /gene="ykiE"
                     /locus_tag="ACHKXP_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO7649426.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            236..757
                     /gene="fecI"
                     /locus_tag="ACHKXP_004573"
     CDS             236..757
                     /gene="fecI"
                     /locus_tag="ACHKXP_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO7649427.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            754..1707
                     /gene="fecR"
                     /locus_tag="ACHKXP_004574"
     CDS             754..1707
                     /gene="fecR"
                     /locus_tag="ACHKXP_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO7649428.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1794..4118
                     /gene="fecA"
                     /locus_tag="ACHKXP_004575"
     CDS             1794..4118
                     /gene="fecA"
                     /locus_tag="ACHKXP_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO7649429.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIASYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4163..5065
                     /gene="fecB"
                     /locus_tag="ACHKXP_004576"
     CDS             4163..5065
                     /gene="fecB"
                     /locus_tag="ACHKXP_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO7649430.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNKTYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5062..6081
                     /gene="fecC"
                     /locus_tag="ACHKXP_004577"
     CDS             5062..6081
                     /gene="fecC"
                     /locus_tag="ACHKXP_004577"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO7649431.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWL
                     VRRRG"
     gene            6078..7034
                     /gene="fecD"
                     /locus_tag="ACHKXP_004578"
     CDS             6078..7034
                     /gene="fecD"
                     /locus_tag="ACHKXP_004578"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO7649432.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7035..7802
                     /gene="fecE"
                     /locus_tag="ACHKXP_004579"
     CDS             7035..7802
                     /gene="fecE"
                     /locus_tag="ACHKXP_004579"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO7649433.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8360..8617)
                     /locus_tag="ACHKXP_004580"
     CDS             complement(8360..8617)
                     /locus_tag="ACHKXP_004580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649434.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8700..>8926)
                     /locus_tag="ACHKXP_004581"
     CDS             complement(8700..>8926)
                     /locus_tag="ACHKXP_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO7649435.1"
                     /translation="CWDNSPMERFFRSLKNEWVPATGYVSFSDAAHAITDYIVGYYSA
                     LRPHEYNGGLPPNESENRYWKNSNAEASFS"
BASE COUNT         1939 a         2546 c         2476 g         1965 t
ORIGIN      
        1 aaatccccat cctttagggc agtgaggatg tcaataatag cagaccacat catgagtcat
       61 tctgaccgac acatgccaga agcagaggct ccatcccatt ttatacctac cttataacac
      121 ttagaaaaac aacatgttaa aaatgtctat tggaaacaat tttattccca attgtaatga
      181 taaccattct catattaata taactacgtg ataattaact tttgatgcac tccgcatgtc
      241 tgaccgcgcc actaccacag cttccttaac gttcgagtcg ctttatggca cacatcacgg
      301 ctggttgaaa agctggctga cgcgcaaact ccagtctgct tttgatgcag atgacattgc
      361 ccaggacact tttttgcggg taatggtcag cgaaacgctc tcgacgatcc gcgatcctcg
      421 ctccttcctc tgcactatcg ccaaacgcgt gatggtggac ctgtttcgcc gaaacgcgct
      481 ggaaaaagcg tatctggaga tgctggcgct tatgccggag gggggagcgc cttcacctga
      541 ggaacgcgaa agccaactcg agaccctaca actcctcgac agcatgctgg acgggctaaa
      601 cggcaaaaca cgtgaagcgt ttctgctttc gcaactggat ggtctgacat acagcgagat
      661 tgcgcacaaa ctcggtgttt ccatcagctc cgtgaaaaaa tacgtggcga aagccgtcga
      721 gcactgcctg ctgttccgtc tggagtatgg gttatgaatc ctttgttaac cgattcccgc
      781 cgtcaggcgc tgcgttcagc ttcccactgg tatgccgtgc taagcggcga gcgcgtcagc
      841 ccacaacagg aagcgcgctg gcaacagtgg tatgaacagg atcaggataa ccagtgggcc
      901 tggcagcagg ttgaaaacct gcgcaaccag cttggcggtg tgcctggcga cgttgccagc
      961 cgggcgttgc acgatacccg cctcacccgc cgtcacgtga tgaaaggatt actgctgttg
     1021 ctcggcgctg gcggaggctg gcagctctgg cagtcggaaa ccggcgaagg tctgcgggca
     1081 gattaccgca ccgccaaagg cacagtcagc cgtcagcaac tggaagatgg ctccctgctc
     1141 acgctgaata cccaaagcgc ggcggatgtg cgttttgatg cgcatcagcg caccgtccgg
     1201 ctctggtacg gtgaaatcgc cattactacc gcgaaagatg ccctgcaacg ccccttccgt
     1261 gtcctgaccc gtcagggcca gctcactgct ttagggacag aatttaccgt ccgtcagcag
     1321 gataatttca cgcagcttga cgtgcagcag cacgctgtgg aagtgcttct cgccagtgcc
     1381 cccgcgcaaa aacgcatcgt gaacgctggt gaaagcctgc agttcagcgc ctctgagttt
     1441 ggcgcagtga aaccgctgga tgacgagagt acaagctgga cgaaggacat cctgagcttc
     1501 agcgataaac cgctgggtga ggtgatagcc acgctaaccc gttaccgcaa cggcgtgctg
     1561 cgctgcgatc ccgccgttgc cgggctgcgc ctgagcggga cgttcccgct gaaaaatacc
     1621 gatgcgatcc tgaacgttat cgcgcaaacg cttcccgtta aaattcagtc tattacgcgg
     1681 tactggataa acatttcacc actgtaagga aaataattct tatttcgatt gtccttttta
     1741 cccttctcgt tcgactcata gctgaacaca acaaaaatga tgatggggaa ggtatgacgc
     1801 cgttacgcgt ttttcgtaaa acaacacctt tggttaacac cattcgcctg agcctgctgc
     1861 cgctggccgg tctctcgttt tccgcttttg ctgcacaggt taatatcgca ccgggatcgc
     1921 tcgataaagc gctcaatcag tatgccgcac acagcggatt taccctctcg gttgacgcca
     1981 gcctgacgcg cggcaagcag agcaacggcc tgcacggcga ttacgacgtc gagagcggcc
     2041 tgcaacaact gctggacggc agcggactgc aggtaaaacc gctgggaaat aacagctgga
     2101 cgctggagcc cgcgcccgca ccaaaagaag atgccctgac cgtggtcggc gactggctgg
     2161 gtgatgcgcg tgaaaacgac gtatttgaac atgctggcgc gcgtgacgtg atccgccgtg
     2221 aggatttcgc caaaaccggc gcaaccacca tgcgtgaggt acttaaccgc atccctggcg
     2281 tcagcgcgcc ggaaaacaac ggcaccggca gccacgacct ggcgatgaac tttggcatcc
     2341 ggggcctgaa cccgcgcctc gccagccgct cgaccgtcct gatggacggc atccccgtcc
     2401 ccttcgcccc ttacggtcag ccgcagcttt cactggctcc cgtttcgctc ggcaacatgg
     2461 atgccattga cgtggtacgc ggtggtggtg cggtgcgtta cggaccgcag agcgtgggcg
     2521 gcgtggtgaa ctttgttacc cgtgccattc cgcaggactt tggtatcgag gcgggcgtgg
     2581 aaggtcagct cagcccaacc tcttcacaaa acaacccgaa agagacgcac aacctgatgg
     2641 tgggcggcac agcggacaac ggttttggca ccgcgctgct ctactccggc acgcgcggca
     2701 gtgactggcg cgagcacagc gccacccgca tcgacgacct gatgctgaaa agcaaatatg
     2761 cgccggatga ggtgcacacc ttcaacagcc tgctgcaata ttacgacggt gaagccgaca
     2821 tgcccggtgg cctgtctcgc gcggattacg acgccgatcg ctggcaatcc acccgcccgt
     2881 atgaccgctt ctggggtcgt cgcaagctgg cgagcctggg ctaccagttc cagccagaca
     2941 gccagcataa attcaacatt caggggttct acacccaaac cctgcgcagc ggctacctgg
     3001 agcaaggcaa acgcatcacc ctctcgccgc gtaactactg ggtgcgcggt attgagccac
     3061 gctacagcca gatctttatg atcggccctt ccgcgcacga agtgggcgtg ggctatcgct
     3121 atttgaatga atcaacgcat gaaatgcgtt actacaccgc caccagcagc gggcagttgc
     3181 cgtccggctc aagcccttac gaccgcgata cgcgttccgg caccgaggcg cacgcctggt
     3241 atctggatga caaaatcgac atcggcaact ggaccatcac gccgggtatg cgtttcgaac
     3301 atatcgcgtc ataccagaac aacgccatca caggcacgca cgaagaagtg agctataacg
     3361 caccgcttcc ggcgttgaac gtgctctatc acctgactga cagctggaat ctttatgcaa
     3421 acactgaagg ctcgttcggc accgtacagt acagccagat tggcaaggct gtgcaaagcg
     3481 gcaatgtgga accggaaaaa gcgcgaacct gggaactcgg tacccgctac gacgacggcg
     3541 cgctgacggc ggaaatgggg ctgttcctga ttaactttaa caatcagtac gactccaacc
     3601 agaccaacga caccgtcact gcacgtggca aaacgcgcca taccgggctg gaaacgcagg
     3661 cacgttacga tctgggtacg ctaacgccaa cgcttgataa cgtttccatc tacgccagct
     3721 atgcgtatgt gaacgcggaa atccgcgaga aaggcgacac ctacggcaat ctggtaccat
     3781 tctccccgaa acataaaggc acgctgggcg tggactacaa gccaggaaac tggacgttca
     3841 atctgaacag cgatttccag tccagccagt ttgcggataa cgccaatacg gtgaaagaga
     3901 gcgccgacgg cagtaccggc cgcattcccg gcttcatgct ctggggcgca cgcgtggcgt
     3961 atgactttgg cccgcagatg gcagatctga acctggcatt cggtgtgaaa aacatcttcg
     4021 accaggacta cttcatccgc tcttatgacg acaacaacaa aggcatctat gcaggccagc
     4081 cgcgcacgct gtatatgcag gggtcgttga agttctgatg tgttaacgcc cggcttgccg
     4141 ggcttttagc tggaatgtga ttatgttggc atttatccgt tttctttttg caggcctgct
     4201 gctggtgatc agccacgcct ttgccgccat ggttcaggac gaacacggca cgtttacact
     4261 cgaaaaaacg ccacaacgga ttgtggtgct ggaactctcg ttcgccgatg cgctggccgc
     4321 cgtggacgtc agcccgatcg gtattgccga cgataacgat gcaaaacgca tcctgcccga
     4381 agtgcgtgcg cacctgaaac cgtggcagtc cgtcggaacg cgcgcgcagc cgagcctgga
     4441 agccattgcc gctctgaaac cagacctgat cattgccgac agcagtcgcc atgcgggggt
     4501 ttacatcgcc ttgcagcaaa tcgcgccggt actgctgctt aagtcccgca acaaaaccta
     4561 cgctgaaaat ttgcaatctg cggctatcat cggcgaaatg gtgggtaaaa agcgagagat
     4621 gcaggcacgt ctggaacaac ataaagagag gatggcgcag tgggccagcc agcttcccaa
     4681 agggacacgc gtggcctttg gcacatcacg cgaacagcaa ttcaacctgc atactcagga
     4741 gacctggacc ggcagcgtgc tggcctctct ggggctgaac gttcccgctg cgatggcggg
     4801 tgcgtccatg ccgtccatcg gcctggagca actgctggcg gtcaatcctg cctggctgct
     4861 ggttgcccac tatcgcgaag agagcattgt taaacgctgg caacaagatc cgctctggca
     4921 gatgttaacc gccgcgcaga agcagcaggt tgcttcggtc gacagtaaca cctgggcgcg
     4981 gatgcgcggt atttttgctg cagagcgtat tgccgctgac acggtaaaaa tcttccacca
     5041 tcagccgctt accgttgtga aatgaccgcg ataaaacacc cggtgctgct gtgggggctt
     5101 cccgttgcag cacttattat tattttctgg ctgagtctgt tttgctactc ggccattcct
     5161 gtttccggag cagatgcaac ccgcgccctg ctgcctggac acacgccaac gctaccagaa
     5221 gcgctggtgc aaaaccttcg tttgccacga agcctggtcg ccgttctgat cggcgcaagc
     5281 ctggcgctcg cgggcacgct gctgcaaacc ctgacccaca acccaatggc ctctccttca
     5341 ctgctcggca ttaacagcgg cgcggcgctg gctatggcgc ttaccagcgc gctgagtccg
     5401 acgccgattg caggctattc tctgtcgttc atcgcggcat gcgggggcgg cgtgagctgg
     5461 ctgctggtca tgaccgcagg aggcggattt cgtcataccc atgacagaaa caaactgatc
     5521 ctcgcgggta tcgcgctgtc ggccttttgt atgggcctta cccgcatcac cctgctgctg
     5581 gccgaagatc atgcttacgg catcttttac tggctggcag gcggagtgtc ccacgcccgc
     5641 tggcaggatg tctggcagct cttgccggtg gtggtcactg cagtccctgt cgtgttactg
     5701 ctggcgaatc aactgaacct gctcaacctc agcgacagca ccgcccatac gctgggagtg
     5761 aacctgacga ggctacgttt ggtcatcaat atgttagtgc tgcttctggt tggtgcgtgc
     5821 gtcagcgtgg caggtccggt ggcgtttatc ggtctgctgg tgccacatct ggcgcgcttc
     5881 tgggcaggct tcgatcagcg caacgtactg ccagtgagca tgctgctggg ggccacgctg
     5941 atgctgctgg gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc
     6001 cccggagatc tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg
     6061 cttgtgagga ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg
     6121 gctgtgcgct gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga
     6181 ccgactggca ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct
     6241 tgctgctggc actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga
     6301 ttgtgcgcaa ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg
     6361 cctctgtggg ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg
     6421 cctttgcggg cggcatggcg gggttgatat tactgaagat gctggcaaag acccaccagc
     6481 cgatgaagct ggcgctcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt
     6541 atctgatgct ctcgcgcccg caggatgtga acaacgccct gctgtggctg accggcagct
     6601 tatggggccg tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc
     6661 cgctgagcct gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca
     6721 ccacgctcgg tgtgtcggtg ccccataccc gattctgggc tttgttacta gctgtcgcca
     6781 tgacatctac cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc
     6841 atatgatgcg tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcggccctga
     6901 caggtgcgtt gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg
     6961 agctcccggt tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg
     7021 tgagaatgcg ataaatgact ttacgaactg aaaatctgac ggtcagttac gggacagaca
     7081 aggtacttaa cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc
     7141 ctaacggttg cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt
     7201 ctggcaccgt atttctcggc gataatccca taaatatgct ctcatcacgc cagttggccc
     7261 gcaggctttc gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc
     7321 tggtttcgta tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca
     7381 atgcacgagt taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt
     7441 taaccgagct ttccggcggt cagcgccagc gcgcatttct ggcgatggtc ctggcccaga
     7501 atacgcccgt tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg
     7561 acctgatgcg gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc
     7621 acgaccttaa tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg
     7681 ttatggcgca aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca
     7741 gcgtggaagc ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaggt
     7801 agattgcaca ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa
     7861 gcctgttcag actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc
     7921 catccgcttg ctttaggctg acaggttcat aatcaacgca aaccagagct gtacaggctt
     7981 gggcgcggct ttcaaaccag tcgtgatcac agcaatcaat tttgaactct gcttaacgga
     8041 catttctgta taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa
     8101 aacgtgacat gactaagttt agatttcagg gggggagatc aaaaaatttc gctctgtgcc
     8161 agagcggaca ttcacggagc tggttcatta ccaatgaggt tgggcttttg aggataaatc
     8221 aatgatcaga cgccaacgta aatcaaaagc aaccctggaa cggaattgct aatccagttt
     8281 ctgaccatcg atttttctaa aaagtgtgcg tttgctacta cttaggtagg tgcagctttc
     8341 ttaatcaccg gcagccacgt tatacaggcc agttgatgga tcgattgtta tcaatgatat
     8401 cttcatgagt cggtgtctca cccagcttac cgaagctggc ataaagtgaa ggcagacggg
     8461 cccgtccttc tccctttttc gccagaggga aagcgcgaag catggtggca gcctcccagt
     8521 cacaacaata ggatggtgtg cacggctgct gacgccatga ttcagcgata gagccggaaa
     8581 atacggggtc aaagccggta tcattaacca gagtcatcgt gatctgtact gcggctggat
     8641 gatctcctgc tactgcaact gccaggcgac cgcggctccc ttcaagtagt ctgttgtggt
     8701 caactaaaac tggcctccgc gttagagttt ttccagtatc ggttttctga ttcgtttggt
     8761 ggtaacccac cattatattc gtgcggtctt agtgcgctgt aatatccaac gatatagtcc
     8821 gttatggcgt gagctgcatc gctgaagctt acatagcccg tcgctggcac ccattcgttc
     8881 ttcagactcc tgaagaagcg ctccattggg ctgttatccc agcagt
//
LOCUS       DAZFZC010000151         8899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFZC010000151 DAZFZC010000000
VERSION     DAZFZC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.153"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..470
                     /locus_tag="ACHKXP_004582"
     CDS             <1..470
                     /locus_tag="ACHKXP_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7649436.1"
                     /translation="TWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQAVWDR
                     RPSGTIHHCDKGSLYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIH
                     RNSWKNRTEVELATLTWVDWYNNRRLLGRLDHIPPAEAEKAYYASIRNDDLAA"
     gene            1511..2383
                     /locus_tag="ACHKXP_004583"
     CDS             1511..2383
                     /locus_tag="ACHKXP_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649437.1"
                     /translation="MVKVVEDERSRIRYLERRLNENGFYLPSSLADKDYLSYQKRILN
                     TLISQGVDTLKINNFLAETDQRYFDSLPSENDLNWYRNDARASLWLTCELYEMIKING
                     YENTLTCLSPESLPSHHSVRVDAIRRCIDNWPFILYTPSNYLNQKSIEWTTLLEKDDI
                     FREVKARNVDICSWLKKYIQEKTNISLNYVCGESSEEIMAWCYASYFTWKKNNQNSPD
                     SVELFTRKFKSAWATQKNRIKNRVDKKLRPLNVNISQEAYDKLRKLSMNEGISNDRVI
                     ESALDMIYRSKIKK"
     gene            2482..3354
                     /locus_tag="ACHKXP_004584"
     CDS             2482..3354
                     /locus_tag="ACHKXP_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7649438.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRYILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            3726..6848
                     /gene="agn43"
                     /locus_tag="ACHKXP_004585"
                     /gene_synonym="flu"
     CDS             3726..6848
                     /gene="agn43"
                     /locus_tag="ACHKXP_004585"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO7649439.1"
                     /translation="MKRHLNTSYRLVWNHITGAFVVASELARARGKRGGVAVALSLAV
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKADGTIINQEGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETRINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYSLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFHARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     SSQATLNVTF"
     gene            6969..>8899
                     /locus_tag="ACHKXP_004586"
     CDS             6969..>8899
                     /locus_tag="ACHKXP_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649440.1"
                     /translation="MLQIVGALLLLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYASLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKV"
BASE COUNT         2299 a         2214 c         2387 g         1999 t
ORIGIN      
        1 gcacatggca gggcttcgtt tatgtggcgt tcatcattga tgtgttcgcc ggatacatcg
       61 tgggatggcg ggtctcatcg tccatggaaa cgacattcgt gctggatgca ctggagcagg
      121 cagtatggga ccgtcgtccg tctggcacta tccatcactg tgataaaggt tctctgtatg
      181 tatcgctggc ctacacgcag cggcttaagg aagcaggatt actggcatca acagggagca
      241 caggcgactc gtatgacaac gcgatggcgg agagcatcaa tggtctttac aaagcggagg
      301 taatacaccg taatagctgg aaaaaccgga cagaagtgga actggccaca ctaacgtggg
      361 tggactggta taacaatcga cgattgctgg gaaggctgga tcatatccct ccggcagaag
      421 cagaaaaagc ttattatgct tccatcagaa acgatgatct ggcagcctga gttcacaaat
      481 aaaacaatct ccaggaaacc cggggcggtt caaatttcgc agagggctgg cttctgatgc
      541 tgacgctaac gtgcttttcc cacctgctaa ccagaaagta atctgcccag cataacagta
      601 accccatcag cttaaaatca ataacgccct gtaaaacctg aagtaacagt ccgggaactc
      661 gtatcatgct aacatatcag tgttgtgaag cagtcgacaa cgagcttcag acggtacaca
      721 cgtcagttca tgcagaaata gcggccatat caggtctggc tgtcagcgaa ccacgcagcc
      781 tgagtacatt gttgtaaatt aaacgactgg tgatttttaa attttttctt tgccaccacc
      841 gtgaactaat accgaaaaaa cagtagtctc aggaagcgag ttctgaaagt cagctttatt
      901 aaaataagct attagaccgg gtaagtaatg tcaacgaatg aaatgttatg ataattctgg
      961 ataccatatc tgtaagagtg attaatgagg aaagtgatga cttttaatta ttttatctcc
     1021 ggtaacgtaa aaccagactt atcgtaaatg aaattatgaa gtcctctgtt aacgtcatgg
     1081 agagacgcat caaaatacag gatacaatga ttctcacaat gaaactcatc gtctctggtg
     1141 aaattcatat caacatttac cttaataacc tgacgcggga ttaactgtgt tgtttaaggc
     1201 aaagggggga gttgcccgaa ggcgaaaacg aaagtaagtt aataataaca ggatgagagg
     1261 agaaagcgct cttcccggaa aataaaaaga gcggttcact cctgtgaatg cacttcaacc
     1321 gtcctggcaa agaatgtact gttataaaaa actcccccag tcagcagaga caaaaagata
     1381 acatgaggta cctcccaccc ccttaattta tacacgtgaa atgaactgaa atttatgaga
     1441 aaatcatgct aaagatgctt tgattttact taaaatcacg tgtgattatc ctgactgaag
     1501 gagagatact atggttaagg ttgtggaaga tgaaagaagc agaatacgat atcttgaaag
     1561 acgcttaaat gaaaatgggt tttatttacc atcaagcctg gccgataaag attatttatc
     1621 gtatcagaaa cggattttaa acactcttat ttcacagggt gttgacaccc taaaaataaa
     1681 taattttctg gctgaaacag accagcgtta cttcgattcc ttaccatctg agaatgatct
     1741 taactggtat cgtaatgatg cccgcgccag tttatggctg acttgtgaat tatatgaaat
     1801 gattaagata aacggttatg aaaataccct tacctgttta tctccggagt cactaccttc
     1861 tcaccacagc gtgagggtag atgccatacg caggtgtatt gataactggc ctttcatttt
     1921 atacacacca tcaaattacc tgaaccagaa aagtattgaa tggaccacac tgctcgaaaa
     1981 agatgatatt ttcagagagg ttaaggccag aaatgttgat atctgctcat ggctcaaaaa
     2041 atatattcag gaaaagacga atatttccct gaactatgtc tgtggagaat catctgaaga
     2101 gattatggcc tggtgttatg ccagttactt cacatggaaa aaaaacaacc agaattcacc
     2161 agactcagta gaactgttta cccggaagtt taaaagcgcc tgggctaccc aaaaaaacag
     2221 aataaaaaac agggttgata aaaagctgcg ccccctgaac gtaaatattt cacaggaggc
     2281 atatgacaaa ctgagaaaat tatccatgaa cgagggtatc tcaaatgatc gggttattga
     2341 aagtgctctg gacatgattt accgtagtaa aattaaaaaa taaaaccatt caaaataatt
     2401 tcagacagat attatctccg tgaatccccc gccacctttc cggtgtgcgg ggtcttgtct
     2461 tttttcaccg ggaatacatg tatgaatccg tctgatgcca ttgaggcaat tgaaaaaccg
     2521 ctctcctccc tgccttactc actttcccgt cacatcctgg aacatctgcg caaactcacc
     2581 agtcacgaac ccgtgattgg cattatgggt aaaagcgggg ccggtaaatc ctcactctgt
     2641 aatgcactgt ttcaggggga ggtcaccccg gtcagtgatg ttcacgccgg cacccgggaa
     2701 gtgcggcgct tccgtctgag tggccacggt cacagcatgg ttatcactga cctgcccggg
     2761 gtgggcgaga gccgggacag ggatgcagag tatgaagccc tgtaccgtga cattctgccc
     2821 gaactggacc tggtgctgtg gctgattaaa gccgatgacc gcgccctgtc tgtggatgag
     2881 tatttctggc gatacatcct gcaccgggga catcagcagg tgctgtttgt ggtgacgcag
     2941 gccgacaaaa cggagccctg ccatgaatgg gatatggccg gcattcagcc ttctcccgca
     3001 caggcacaga acattcggga gaaaacggag gcggtattcc gtctgttccg gcccgtacat
     3061 ccggttgttg ccgtatcggc ccgcaccggc tgggaactgg atacgctggt cagtgcgcta
     3121 atgacagcac ttcccgacca tgccgccagt cccctgatga cccgcctgca ggacgagctg
     3181 cgcacggaat ctgtccgcgc tcaggcccgt gaacagttta ccggtgcggt ggaccggata
     3241 tttgacacgg cggagagcgt ctgtgttgcc tctgttgccc gtacggttct gcgtgccgtc
     3301 cgtgacacgg tggtctctgt tgcccgcgct gtatggaact ggattttctt ctgaacactc
     3361 tgccagggga aatgcctgat tacattgcgt taaccaactg aaaacatgac acaaccccct
     3421 catacccacc tctttctctt gcatgactgt cttgctgtta atagaataaa acgatcgata
     3481 aaacaggtat cgatcgttga tatcgatcat ttatagtggt taatggcctg caaaaaggca
     3541 gctgaatctc ttcatcatgc agaacggaat tgcacacaat ggactgatac ttctctggcg
     3601 gatgagagag tggaagcatt tatgtccggg aaaaaccact ttatctgacc atgctgtttt
     3661 gtacctgccg gtatccacat ttgtgggtac cggctttttt attcaccctc tgtaaggaaa
     3721 agctgatgaa acgacatctg aacaccagct acaggctggt atggaatcac attacgggcg
     3781 ctttcgtggt tgcctccgaa ctggcccgcg cacggggtaa acgtggcggt gtggcggttg
     3841 cactgtctct tgccgtggtc acgtcactcc cggtgctggc tgctgacatc gttgtgcacc
     3901 cgggtgaaac agtgaatggc ggaacactgg taaaccatga caaccagttt gtatccggaa
     3961 cagctgatgg cgtgactgtc agtaccgggc ttgagctggg gccggacagt gacgaaaaca
     4021 ccggcgggca atggataaaa gcgggtggca caggcagaaa caccactgtc accgcaaatg
     4081 gtcgtcagat tgtgcaggca ggaggaactg ccagtgatac ggttattcgt gatggcggag
     4141 ggcagagcct taacggactg gcggtgaaca ccacgctgga taacagaggt gagcagtggg
     4201 tacacggggg agggaaagct gacggtacaa ttattaacca ggagggttac cagaccataa
     4261 aacatggcgg actggcaacc ggaaccatcg tcaacaccgg tgcagaaggt ggtccggagt
     4321 ctgaaaatgt gtccagcggt cagatggtcg gagggacggc tgaatccacc accatcaaca
     4381 aaaatggccg gcaggttatc tggtcttcgg ggatggcacg ggacaccctc atttacgccg
     4441 gtggtgacca gacggtacac ggagaggcac ataacacccg gctggagggg ggtaaccagt
     4501 atgtacacaa cggtggcacg gcaacagaaa cgcggataaa ccgtgatggc tggcaggtga
     4561 ttaaggaagg aggaactgcc gcgcatacca ccatcaacca gaaaggaaag ctgcaggtga
     4621 atgccggcgg taaagcgtct gatgtcaccc agaacacggg cggtgcactg gttaccagta
     4681 cagctgcaac cgtcaccggc acaaaccgcc tgggagcatt ctccgttgtg gcgggtaagg
     4741 ctgataatgt cgtactggaa aatggcggac gtctggatgt gctgagcgga cacacagcca
     4801 ctaatacccg tgtggatgat ggcggaacgc tggatgtccg caacggtggt gccgccacca
     4861 ccgtatccat ggggaatggc ggtgtactgc tggccgattc cggtgccgct gtcagtggta
     4921 cccggagcga cggaaaggca ttccgtatcg gaggcggtca ggcggatgcc ctgatgctgg
     4981 aaaaaggcag ttcattcacg ctgaacgccg gtgatacggc cacggatacc acggtaaatg
     5041 gcggactgtt caccgccagg ggcggcacac tggcgggcac caccacgctg aataacggcg
     5101 ccatacttac cctttccggg aaaacggtga ataacgatac cctgaccatc cgtgaaggtg
     5161 atgcactcct gcagggaggc gctcttaccg gtaacggcag ggtggaaaaa tcaggaagtg
     5221 gcacactcac tgtcagcaac accacactca cccagaaaac cgtcaacctg aatgaaggca
     5281 cgctgacgct gaacgacagt accgtcacca cggatgtcat cgctcagcgc ggcacggccc
     5341 tgaagctgac cggcagcaca gtgctgaacg gtgccattga ccccacgaat gtcaccctga
     5401 catccggtgc cacctggaat atccccgata acgccacggt tcagtcggtg gtggatgacc
     5461 tcagccatgc cggacagatt catttcacct ccgcccgcac agggaagttc gttccgacaa
     5521 ccctgcaggt gaaaaacctg aacgggcaga atggcaccat cagcctgcgt gtacgcccgg
     5581 atatggcaca gaacaatgct gacagactgg tcattgacgg tggcagggca accggaaaaa
     5641 ccatccttaa cctggtgaac gccggcaaca gtggtacggg gctggcgacc accggtaagg
     5701 gtattcaggt ggttgaggcc attaacggtg ccaccacgga ggaaggggcc tttgtccagg
     5761 ggaatatgct gcaggccggg gcctttaact actccctcaa ccgggacagt gatgagagct
     5821 ggtatctgcg cagtgaagaa cgttatcgtg ctgaagtccc cctgtacgcc tccatgctga
     5881 cacaggcaat ggactatgac cggattctgg caggctcccg cagccatcag accggtgtaa
     5941 acggtgaaaa taacagcgtc cgtctcagca ttcagggtgg ccatctcggt cacgataaca
     6001 acggcggtat tgcccgtggg gccacgccgg aaagcagcgg cagctatggc ttcgtccgcc
     6061 tggagggtga cctgctgaga acagaggttg ccggtatgtc tgtgaccgca ggggtatatg
     6121 gtgctgcagg ccattcttcc gttgatgtta aggatgatga cggctcccgc gccggcacgg
     6181 tccgggatga tgccggcagc ctgggcggat atatgaacct gacacacacc tcctccggcc
     6241 tgtgggctga cattgtggca cagggaaccc gccacagtat gaaagcgtca tcgggcaata
     6301 acgacttcca cgcacggggc cggggctggc tgggctcact ggaaaccggt ctgcccttca
     6361 gtatcactga taacctgatg ctggagccac ggctgcagta cacctggcag gggctttcgc
     6421 tggatgacgg taaggacaac gccggttatg tgaagttcgg gcatggcagt gcacagcatg
     6481 tgcgtgccgg tttccgtctg ggcagccaca acgatatgac ctttggtgaa ggcacctcat
     6541 cccgtgcccc cctgcgtgac agtgcaaaac acagtgtgcg tgaactgccg gtgaactggt
     6601 gggtacagcc ttccgttatc cgcaccttca gctcccgggg tgatatgcgt gtggggacct
     6661 ccaccgcagg cagcgggatg acgttctctc cctcacagaa tggcacttca ctggatctgc
     6721 aggccggact ggaagcccgt gtccgggaaa atatcaccct gggcgttcag gccggttatg
     6781 cccacagcat caacggcagc agcgctgaag gttatagcag tcaggccacg ctgaatgtga
     6841 ccttctgata attctgcatt gcctctctgt ggtcccggtc atcatgaccg ggacccggac
     6901 gggggaaaac gtttcagtgc cacattcact gtcattcaca ataacatgat attcatcacg
     6961 gagtgactat gttacagata gtcggcgcgc tgctcctgct gatcgcagga tttgccattc
     7021 ttcgcctttt gttcagagca ttaaccagca cggcgtctgc gctggcagga ttcgtattgc
     7081 tgtgtctgtt cggcccggct ttactggctg gctatatcac tgaacgcata acccggttat
     7141 tccatattcg ctggctggca ggcgtatttc taacgattgc cggaatgatc atcagcttca
     7201 tgtggggact tgatggtaaa catatcgcac tggaggctca tacttttgac tctgtaaaat
     7261 ttattctgac caccgctctc gccgctggtc tgctggctct tcccgtgcag ataagaagca
     7321 ttcagcagaa cgggctcaca cctgaagata tcagcaagga aattaacggg tattactgct
     7381 gtttttatac tgcttttttc cttatggcgt gttctgcata cgcatcattg atcgcattgc
     7441 agttcgatat ttcaccctca ctgatgtggt ggggcgggtt gttgtactgg ctggctgcat
     7501 tagtgacgct gctatgggcg gccagccaga tccaggcgct gaaaagactg accagtgcca
     7561 tccgccagac actggaagaa caaccggtgc tcaacagtaa atcgtggctg agcagtttgc
     7621 aaaacgatta cagccttcct gaaacgctga cggagcgcat ctggctgaca ctcatttcac
     7681 aacggatttc ccggggagaa ctgagggaat ttgaactggc agacggaaac tggctactgg
     7741 acaatgcctg gtatgaaaga aacatggcgg gtttcaacga aaagctgaga gagagcctgt
     7801 catttacccc tgatgaactg aaaaccctct tccggaaccg cctgaattta tcaccggaag
     7861 cgaatgacga ttttctcgat cgttgcctgg acggcggtga ctggtacccc ttttcagaag
     7921 gccgccgttt tgtatcattc caccacgtgg atgagcttcg tatctgtgcc tcctgcgggc
     7981 tgacagaagt acatcatgcc ccggaaaatc ataagccgga tccggaatgg tactgctcct
     8041 ctctttgtcg cgaaacagaa acactgtgtc aggacattta tgaacgttct tacaccggtt
     8101 ttatttccga tgcaacggcg aatggtctga ttctcatgaa actgccggaa acctggagta
     8161 caaatgagaa aatgtttgct tccggagggc agggacatgg gtttgccgct gaacgtggaa
     8221 accatattgt cgacagagtc cgtctgaaaa acgcacggat cctcggtgat aataatgcca
     8281 gaaatggagc agacagactg gtcagcggaa cagaaatcca gacgaaatat tgttcaactg
     8341 cagcccgtag cgtcggtgcg gcattcgacg gacaaaacgg acagtatcgt tacatgggaa
     8401 atcatggtcc catgcaactg gaagtccccc gtgatcagta tgccggcgct gtggaaacca
     8461 tgaagaataa gatccgcgaa ggtaaagtac ccggtgtaac cgatcccgca gaagcgtccc
     8521 ggctgattcg tcggggacat ctgacttata cccaggcccg taatatcacc cggttcggga
     8581 ccatcgaatc ggtcacttat gatattgccg aggggtcggt tgtcagtctg gcggccggag
     8641 ggatcagttt tgccctgacg gcatcggtct tctggctcag caccggcgat cgcgatgctg
     8701 ccctgcagac agctgctgtc caggcaggaa aaaccttcac ccgcacactg gctgtctacg
     8761 tcacaaccca gcaacttcac cggctcagtg ttgttcaggg tatgctgaag catattgatt
     8821 tttcgacggc cagcccgact gtccggcagg cgcttcagaa ggggaccggt gcaggaaata
     8881 tcagtgccct gaacaaagt
//
LOCUS       DAZFZC010000152         8843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFZC010000152 DAZFZC010000000
VERSION     DAZFZC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.154"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            206..856
                     /gene="csgD"
                     /locus_tag="ACHKXP_004587"
     CDS             206..856
                     /gene="csgD"
                     /locus_tag="ACHKXP_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO7649441.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATTLLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            861..1250
                     /gene="csgE"
                     /locus_tag="ACHKXP_004588"
     CDS             861..1250
                     /gene="csgE"
                     /locus_tag="ACHKXP_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO7649442.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1275..1691
                     /gene="csgF"
                     /locus_tag="ACHKXP_004589"
     CDS             1275..1691
                     /gene="csgF"
                     /locus_tag="ACHKXP_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO7649443.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1718..2551
                     /gene="csgG"
                     /locus_tag="ACHKXP_004590"
     CDS             1718..2551
                     /gene="csgG"
                     /locus_tag="ACHKXP_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO7649444.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2615..3106)
                     /gene="ycdZ"
                     /locus_tag="ACHKXP_004591"
     CDS             complement(2615..3106)
                     /gene="ycdZ"
                     /locus_tag="ACHKXP_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7649445.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3208..3762)
                     /gene="ycdY"
                     /locus_tag="ACHKXP_004592"
     CDS             complement(3208..3762)
                     /gene="ycdY"
                     /locus_tag="ACHKXP_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO7649446.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3786..4523)
                     /gene="ycdX"
                     /locus_tag="ACHKXP_004593"
     CDS             complement(3786..4523)
                     /gene="ycdX"
                     /locus_tag="ACHKXP_004593"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO7649447.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4578..5516)
                     /gene="ghrA"
                     /locus_tag="ACHKXP_004594"
     CDS             complement(4578..5516)
                     /gene="ghrA"
                     /locus_tag="ACHKXP_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO7649448.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
     gene            5750..5837
                     /locus_tag="ACHKXP_004595"
     tRNA            5750..5837
                     /locus_tag="ACHKXP_004595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5784..5786,aa:Ser,seq:gga)
     gene            complement(5987..6829)
                     /locus_tag="ACHKXP_004596"
     CDS             complement(5987..6829)
                     /locus_tag="ACHKXP_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7649449.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRGLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDDVFERGVINVFRVLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLNSGQQADRLTDLERMLHLFSGKPIPDNR
                     ENITIHLDEHIQSVQGKEDYEDEMFSIRYFKKGSAYITFRKPELVERLNDIIAEHYPD
                     MLSV"
     gene            complement(6914..7111)
                     /locus_tag="ACHKXP_004597"
     CDS             complement(6914..7111)
                     /locus_tag="ACHKXP_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7649450.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQQQLLQQNR"
     gene            complement(7128..7616)
                     /locus_tag="ACHKXP_004598"
     CDS             complement(7128..7616)
                     /locus_tag="ACHKXP_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7649451.1"
                     /translation="MNLSLTPEADSINMQALNMGRIVVDVDGVNLAELINKVSENGYS
                     LRVVDKSDQQSTSTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(7613..7996)
                     /locus_tag="ACHKXP_004599"
     CDS             complement(7613..7996)
                     /locus_tag="ACHKXP_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7649452.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGFSAGAPSSQLINSIDILRARRAT
                     GLMTRDNYRMVNNITLGKHPEEAKQ"
     gene            complement(8077..8457)
                     /locus_tag="ACHKXP_004600"
     CDS             complement(8077..8457)
                     /locus_tag="ACHKXP_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7649453.1"
                     /translation="MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYLAVYPATETESNPPE"
     gene            complement(8468..>8843)
                     /locus_tag="ACHKXP_004601"
     CDS             complement(8468..>8843)
                     /locus_tag="ACHKXP_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitoxin of the YeeV-YeeU toxin-antitoxin
                     system"
                     /protein_id="HFO7649454.1"
                     /translation="YAPQDRELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYCMDI
                     TVTRNSPTGQAMTTDAEAAVSEALRDLAFWLYRQLENECDWLTSDAAVDEALLINEYT
                     FTEAGLRAGCPVIVKLSFTDFL"
BASE COUNT         2242 a         2201 c         2152 g         2248 t
ORIGIN      
        1 tacatttagt tacatgttta acacttgatt taagatttgt aatggctaga ttgaaatcag
       61 atgtaatcca ttagttttat attttaccca tttagggctg atttattact acacacagca
      121 gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc aggtgtgcga
      181 tcaataaaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc atggtcatac
      241 attattgttg atcactaaac cttctttgca ggcgacaact ctcttgcagc accttaaaca
      301 atcgctggca attacaggaa aattacataa tattcaacgt tctctggacg atatctcttc
      361 aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc attattggca
      421 ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata cgcctgaaga
      481 ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt attccatgga
      541 ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgcggcgaat gctactttac
      601 gcaaaagctt gccagttacc tgattacgca ttcaggtaac tatcgttata acagcacgga
      661 atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta tcggcgcgtc
      721 taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa cgcatcttta
      781 taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct gggcaaacga
      841 taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg gcagaatttc
      901 tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg ctaactgacc
      961 atactgtttc atctattggc catgattttt accgagcctt tagtgataaa tgggaaagtg
     1021 actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga agctggatca
     1081 ctataacggt caatcaggac gttattttcc agactttttt atttccgttg aaaagagact
     1141 tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat cgtcgccaga
     1201 taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa ataaaaaatt
     1261 gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt atttcgccat
     1321 taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt ggtaacccaa
     1381 ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa gatccgagct
     1441 ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact caggccatcc
     1501 agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg ggccgcatgg
     1561 tgaccaacga ttatattgtc gatattgcca accgcgatgg tcaattgcag ttgaacgtga
     1621 cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa aataactcaa
     1681 ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat ttcttttggt
     1741 tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg ccagaccgac
     1801 attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc cgacgggtaa
     1861 aatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac cctacccggc
     1921 aagtaacttc tccactgctg ttccgcaaag cgccacggca atgctggtca cggcactgaa
     1981 agattctcgc tggtttatac cgctggagcg ccagggctta caaaacctgc ttaacgagcg
     2041 caagattatt cgtgcggcac aagaaaacgg cacggttgcc attaataacc gaatcccgct
     2101 gcaatcttta acggcggcaa atatcatggt tgaaggttcg attatcggtt atgaaagcaa
     2161 cgtcaaatct ggcggggttg gggcaagata ttttggcatc ggtgccgaca cgcaatacca
     2221 gctcgatcag attgccgtga acctgcgcgt cgtcaatgtg agtaccggcg agatcctttc
     2281 ttcggtgaac accagtaaga cgatactttc ctatgaagtt caggccgggg ttttccgctt
     2341 tattgactac cagcgcttgc ttgaagggga agtgggttac acctcgaacg aacctgttat
     2401 gctgtgcctg atgtcggcta tcgaaacagg ggtcattttc ctgattaatg atggtatcga
     2461 ccgtggtctg tgggatttgc aaaataaagc agaacggcag aatgacattc tggtgaaata
     2521 ccgccatatg tcggttccac cggaatcctg acagataaaa aagccggaat tatcatccgg
     2581 cattttactg tttaagcgaa agcgtgagtg cccctcacgc tttatttttg atttcctgct
     2641 cacggtgcgc cgtctttgca ctacgcgccg ccagccacag gccactgttt ttcattgcgt
     2701 aaccaaatat cagcccaagc gccagcgaag gtaacaccag tttccagtca ccctgcccgg
     2761 caaacgtcgc gcatgcgcct ataaacgtcc ccggaacaaa tgaaagcagc agctgtttgg
     2821 cctgaataca catcagaaac gcgacaatac cggttatgac ataaccgaga atttccagat
     2881 gtggtgccag tgcactaccg taaataatga ccatcgccca caccacgcca ctaagcaggg
     2941 ttgcagcgga gatcgccagc cctttcagcc caccttgcgg gcaggcaaag taggccgtac
     3001 agcccaggaa gcccgcccag cttagtaagc ccagggaaac agccacccat ccccagatac
     3061 cggagagaat gcctgttgtg attgcaatag agagaagtat gttcatgccg cgcatgatag
     3121 cagaaaacgc ggccatgaat gagatcgagc acacatttta tgcaacaaca aattatccgt
     3181 tgcagttatg agtgacgtag atcacactta ttcttcagaa tcttcttcca gctcgtccca
     3241 cattgcacta atggcatcgc gggttagcgg tgccatggtg cgccagaaag gcgtggttgc
     3301 atgggcctcc actttgccaa ggaacgcacc acaccagggt aacagatact cactgaacag
     3361 tgtttccagt gcttcgctct catcttccgt tgactgatct tccagccagg aagccgcaag
     3421 cagcaatgtg ccgatgtgat cggctggcgt atccgccagt ggcatccctc gttcggaaag
     3481 aaaagcgcgc acttccgctt ccgttgcgtc ctcaacccat gcgctacgat atggcggcac
     3541 ggcacattca tcgccgataa acaacgcatt gtaatcggca gagacttgcg ccatatcaca
     3601 gcttttctgt aaacgcgtca gtaactcatc ctgctccagt ggccagttcg cagccagttt
     3661 cccctcacga atcagggtaa acagcggcac cagtaaagga tcttgcggtt ggcggtaata
     3721 gagcgaaccc agcacacgac agaggataga aaactcgttc attaatatat ttccattact
     3781 agtaattaaa ggtctgcaaa ttccgcaatc ggtgccatac cgcgagattc aaggaagttc
     3841 agtaagcggc gcggagaaac attcaaaatg cgctctggcg gaaaatctac cgcgtcgagg
     3901 attttaagac actcttcaaa ttcccccatg gtaaacgcgg tgtgagaatc cgagcctaat
     3961 gccacccaac ctccagcatc acgtaccgct gcggctaccg cacgacagtt gtcttcactg
     4021 cccttacgtg agtgtaaaaa tgaagaatta ttgatttcca gcgccacctg atgtttcgcg
     4081 gctgcttcag caacggcttt cacatctatt tcatatttgg gatttccggg atggcttatt
     4141 atatgcacat tgccgctggc gatagtggcg atcatcgctt gtgtattggt cgctttgtca
     4201 tgtggtgcaa aaaccggctc atgaaaaccg gcaataatca gatccagcga gtcaaacatt
     4261 ttaccgctgc aatcaatttc accatcaaca tttttaatgt tagcttcgat gccgcgcagg
     4321 atccctaccc catcaaccac tcgcggccag atacgcatgt taatgaagtg ccagtgatgc
     4381 ggagcatctt ccatatccgg gccatgatcg gtgatcgcaa aaagtttaat gcccttttgt
     4441 ttggcctggg caatgtaatc actcaatgtg ctatatgcat gtgtgctggc aacggtatgc
     4501 atatgaaggt cgacgggata cataactctc tcctgtattc aattcccgcc aaggatagca
     4561 ggaatcctga tgctttatta gtagccgcgt gcgcggtcga cttgcccgca gaccttctcc
     4621 cctttttcga gctgggcaat agtgcgagaa atgtactcca cagcttcagc gggacgggta
     4681 atcgcggcga catgtggtgt tatcgtcacg cgtggatgtt gccagagcgg actttcaggc
     4741 ggtaagggtt cacgattaaa aacatccagc attgcgcctt taactttccc gctatccagc
     4801 gccgcgagca ggtcatcttc cacaacatga acaccacgcg ccaggttgag gagatacgcg
     4861 ccatccggta atttttcgag taattgttga ttaataatgc cgacggtttc aggggtattc
     4921 ggtaacaaat taatcaatac ccgacattgg ctcagaaatg cagacagttc ttcccgtccg
     4981 gcaaagcttt gcacgccagg ccacgattta cgggttcgac tccagcaacg cagcggaaag
     5041 cgccaggttt gcagactctg agcaacttta ctgcccagta cgcctgcgcc caaaatgccg
     5101 atggtaaaat cttcccgatg atattcaggc agcggttgcc aatgcgaact attttgctgg
     5161 atgcgataat cgtcaaaacg tcgaaaccaa tgcagcacct gactgacagc atattcctgc
     5221 atttgctcgc ccataccggt atcttccagg cgaaaaagtg gaacagaagg gttcagcatt
     5281 tcagggtgtg cctgtagctt gctcaaaata gaatcaacac cggccccgag tgcgaacacc
     5341 gctttaagat cgcgccctgc cagcatttca acaggaggat gccagactaa agcataatca
     5401 gcagagtcat tatctccgct tttccatgct ctgactcttg cctgaggaat agctttgcgc
     5461 agtgcctcaa tccaccattg ggtatcgaac gttgggtgat aaaagatgat atccatactg
     5521 actcccgaaa agcgttgtgc gaaatttatt cgcacttatc gttatgatct acaaaagcca
     5581 ccagcataac aaatccgtgg tcggtggcaa aaaaagcaga tttcgcttat taaaactgca
     5641 tattgattga agtttgaata aacgcgcgat tttttaaaaa agtttgttga cctcaggtca
     5701 tgatttccct aaattagcgc ccgttccagc aagacaggaa cgacaatttg gtgaggtgtc
     5761 cgagtggctg aaggagcacg cctggaaagt gtgtatacgg caacgtatcg ggggttcgaa
     5821 tccccccctc accgccagat tacaagcgca tgttattgtt taacatgcgc ttttttattt
     5881 atccttccag acagtatgcc accgggtatg ccatctgaaa ccggtttgta tgagccgcga
     5941 tcacatctta tcggacaatt tccgatcttt aagcatactg gcaaagctat actgacagca
     6001 tgtcaggata gtgttctgca ataatatcat tcagtcgctc aaccagttcc ggttttctga
     6061 acgtgatata cgcggagccc ttcttaaagt atctgatact gaacatctca tcttcatagt
     6121 cctctttccc ctgaacagac tggatgtgct catccagatg aatggtgatg ttttcccgat
     6181 tgtcggggat cggtttgccg ctgaacaggt gcagcattct ttccaggtcg gtaagtcgat
     6241 ctgcctgttg cccgctgttc agatgaaatc cccaccggtc ccacctcacc agattgttga
     6301 cgataatttt actgccaaat ttacagggtg agttggtttt gtaattccag ctcagcactc
     6361 tgaagacgtt gatcactcct cgctcaaaca cgtcatcctt attctggtgc agttgctcaa
     6421 atgtgctcca aatgttcgct tcactgattt ccggaaagtt atcgtactcc agtgagcggt
     6481 accatgtatc acgggcctgc gcatccatta atgacagcat cccggaacgt tgcatcaggc
     6541 cacgccagat ttcgcgatcg agattgcggg tgatggcttt cattgcggtt tccggtttct
     6601 ccatgagcca gcagccgcag cgaaaatcct gtttcatggc ccagtcccgg gcggttttac
     6661 cgccaatgct ttccgtcagt accgagattt cagccagttg ccggataagt gtgtcgattt
     6721 gcagcagcgc actctttcgt acagccagaa tgtggcggat actggttgag cagataacgt
     6781 ccgtatgtcc gttgaccagt tccgggcggg agattgtgat atctgacatc atcgtttttc
     6841 cttttggttt ataaacgaaa acgccagccg tattcaggct ggcgtcagtg gaatgaagtc
     6901 tggtgagtaa gactcacctg ttctgttgca gaagctgctg ttggcgcagg gtacggatat
     6961 cttccctggc ctgttcaatg cagggcagca gtgctgctga atccgtttta tcctcatcgt
     7021 tgtcgaagat aatccctgat tcgcagtcaa tattgtcctg cagccacgca atcagaatat
     7081 ccagcgccgt ttctgtggtt aatgatttca tgttgtgaat ttacgggtta ccagtcgaaa
     7141 gtggggaacc ccggcagaca ttctgcgccg gcgtccagat gaatgagact gacgccataa
     7201 cgccgggtga gagtgacgac cagacgacgg aacgttcttg acaggcccag gcgtttaagc
     7261 cgcagaaccg ggtacgacca tgcatcggta cgtaacagat agccgctgcc tgtaaaatga
     7321 atccattctg tttcgccgtc gtcattagtc tggtgtgaca gcgagtacag ccaggcgttg
     7381 tccgtttccg tgatatgtgc ggtactgcaa cgtatgccgg taagggttgt taacggtggt
     7441 ggtgtactgg ttgactgctg gtcggattta tcgaccacgc ggagtgaata accgttttca
     7501 gagaccttgt taatcagttc agcgagatta acaccatcga cgtcaacgac aatgcgcccc
     7561 atgttcagcg cctgcatgtt aatgctgtcg gcttccggcg tcagggaaag gttcattgtt
     7621 tcgcctcctc cggatgcttg cccagggtaa tgttatttac cattctgtaa ttgtcccggg
     7681 tcatcaggcc ggtcgccctg cgagcccgga gaatatcaat gctgttgatt aactgagacg
     7741 acggggctcc tgcgctgaat cccggctggt cagttcgtac cagcacgtat ttttcaacga
     7801 gaaagttcac cgcgtcacac agtgaaatcc ctgcctcaat atgctgctca atcacacgtt
     7861 catcagcgaa cggtgtgtca ttcaacgtaa ggccatagtg ctggtccagc agtcgggtga
     7921 gcagtgtctg ccagatttca accggagacg ggcagcgcga tgccgcccgt acgtgtgtgt
     7981 cgggtaatgt tttcatactg aagattttcc tgatatgcag atataagaaa tggaaaagtg
     8041 gcgtggctgg tgaaagcacc aggccggagc agaaggctat tccggagggt tactttccgt
     8101 ttcggtcgca ggataaacag ccagataaac gtaaccacag gagccgaggg tgtcggcatc
     8161 gcaggtcagc cctttcgcat acagcgtgac ggtatgctga tgccggggat tcagttcacc
     8221 gctggtgagc atgagttcca gttgtttcat cagcagtgga aaagcctggt ccagatggta
     8281 tgcatccgca ttgctgaacc ggcctctgat accggcgcgg tctgcaaggt aatgcaaccg
     8341 gttaccctcc tgcaccagac gtgccccgaa acagggcgtc acggtgcagg gcagccccca
     8401 ccaggggcgg tctttgttgt cgtcgggaag cgttgtcccg gggagtgtgt ctgacacgat
     8461 aaaatcctca cagaaaatcg gtgaatgata gtttaacgat gacggggcag ccggcgcgca
     8521 gaccggcttc ggtgaaggta tattcgttga tgagaagggc ctcatccaca gcggcgtctg
     8581 aggtcagcca gtcgcactcg ttttccagtt gacggtacaa ccagaaggcc aggtcgcgca
     8641 gggcttcgct gactgcggct tccgcatcgg ttgtcatagc ctgaccagtc ggactgttcc
     8701 gggtgacggt gatatccatg cagtacggat ggcagtaacg tccccggtgg tggatttcag
     8761 cctgcagttg ccagaagtta cgcttctgca ccgcctgcag ggcatccgcg atacgatgaa
     8821 gctcacggtc ctgcggggca tac
//
LOCUS       DAZFZC010000153         8736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFZC010000153 DAZFZC010000000
VERSION     DAZFZC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.155"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..183
                     /locus_tag="ACHKXP_004602"
     CDS             <1..183
                     /locus_tag="ACHKXP_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649455.1"
                     /translation="YQPTSGKEQFWVAKRTADEQWVQLYHAITLFDDLPESAKLGWVS
                     TTNINTLGIFECMTKN"
     gene            complement(391..467)
                     /locus_tag="ACHKXP_004603"
     tRNA            complement(391..467)
                     /locus_tag="ACHKXP_004603"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(431..433),aa:Asp,seq:gtc)
     gene            complement(600..1331)
                     /gene="dnaQ"
                     /locus_tag="ACHKXP_004604"
     CDS             complement(600..1331)
                     /gene="dnaQ"
                     /locus_tag="ACHKXP_004604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO7649456.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1396..1863
                     /gene="rnhA"
                     /locus_tag="ACHKXP_004605"
     CDS             1396..1863
                     /gene="rnhA"
                     /locus_tag="ACHKXP_004605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO7649457.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1860..2582)
                     /locus_tag="ACHKXP_004606"
     CDS             complement(1860..2582)
                     /locus_tag="ACHKXP_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7649458.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2616..3371
                     /gene="gloB"
                     /locus_tag="ACHKXP_004607"
     CDS             2616..3371
                     /gene="gloB"
                     /locus_tag="ACHKXP_004607"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO7649459.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3443..4801
                     /gene="mltD"
                     /locus_tag="ACHKXP_004608"
     CDS             3443..4801
                     /gene="mltD"
                     /locus_tag="ACHKXP_004608"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO7649460.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLIAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQRNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4849..5619)
                     /locus_tag="ACHKXP_004609"
     CDS             complement(4849..5619)
                     /locus_tag="ACHKXP_004609"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7649461.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5697..6497)
                     /locus_tag="ACHKXP_004610"
     CDS             complement(5697..6497)
                     /locus_tag="ACHKXP_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7649462.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6738..7652
                     /gene="yafC"
                     /locus_tag="ACHKXP_004611"
     CDS             6738..7652
                     /gene="yafC"
                     /locus_tag="ACHKXP_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO7649463.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPNYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7649..8452)
                     /gene="dkgB"
                     /locus_tag="ACHKXP_004612"
     CDS             complement(7649..8452)
                     /gene="dkgB"
                     /locus_tag="ACHKXP_004612"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO7649464.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVSRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8615..8691)
                     /locus_tag="ACHKXP_004613"
     tRNA            complement(8615..8691)
                     /locus_tag="ACHKXP_004613"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8655..8657),aa:Asp,seq:gtc)
BASE COUNT         2348 a         2092 c         2110 g         2186 t
ORIGIN      
        1 tatcagccca catcaggaaa agaacaattc tgggtcgcta aacgaacagc tgatgaacaa
       61 tgggtacaac tctaccatgc cataacactt tttgatgatt tacctgaatc agctaaactc
      121 ggatgggtta gtaccacgaa cataaacaca ttggggatat ttgagtgcat gactaagaat
      181 tagcactcat gataaaataa taattgaagg tgggtaacct caccttcatt attgtccatc
      241 ttaattagag ttcactatat acctgtattg cgtccaatac tcactaatgc aattgaagga
      301 gaaccaaaat aaaatggcat actggatggc ataccataat aacctcaaaa acaaaaacgc
      361 catgctaatc aatagcatgg cgttgcaata tggcggaacg gacgggactc gaacccgcga
      421 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccgcg ttgtgttccg
      481 ttgggaacga ggcgaatatt acggattgcc tcacccgtcg tcaatggttt ttctcatctt
      541 ttgaatcgtt tgctgcaaaa atcgcccaag tcgctatttt tagcgccttt cacaggtatt
      601 tatgctcgcc agaggcagct tccgcctttc ttctgcacca gatcgagacg ggcttcatgt
      661 gctgcaagct cttcatctgt cgcaaaaaca acgcgtaact tacttgcctg acgtacaatg
      721 cgctgaattg ttgcttcacc ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc
      781 gacgtttgac caccggtcat cgccagataa acttccgcaa ggatctgggc atcgagtaat
      841 gccccgtgta gcgttcgttt actgttatct atttcgtagc gggcacataa cgcatcgagg
      901 ctgttgcgct tgccaggaaa cattttcctc gccaccgcaa ggctatcggt gaccttacag
      961 aaggtattgg tcttcggaat atcgcgctta agcaacgaga actcgtagtc cataaagccg
     1021 atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc ggatatagtc catgaactca
     1081 tcggctactt cggcaaacgt gggcttatcg agcaaaaatt catcggcaat accatgtacg
     1141 ccaaaggctt ccggatccac cagccgatcg ggtttgagat aaacatggaa gttattgccc
     1201 gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa tgatcttgtg gccttcatag
     1261 tgcgcaccaa tctggttcat accggtggtt tcggtatcga gaacgatctg gcgtgtaatt
     1321 gcagtgctca tagcggtcat ttatgtcaga cttgtcgttt tacagttcga ttcaattaca
     1381 ggaagtctac cagagatgct taaacaggta gaaattttca ccgatggttc gtgtctgggt
     1441 aatcctggac ctgggggtta cggcgctatt ttacgctatc gcggacgcga gaaaaccttt
     1501 agcgcaggct acacccgcac caccaacaac cgtatggagt tgatggccgc tattgtcgcg
     1561 ctggaggcgt taaaagaaca ttgcgaagtc attttgagta ccgacagcca gtatgtccgc
     1621 cagggtatca cccagtggat ccataactgg aaaaaacgtg gctggaaaac agcagacaaa
     1681 aaaccagtta aaaatgtcga tctctggcaa cgtcttgatg ctgcgctggg acagcatcaa
     1741 atcaaatggg aatgggttaa aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa
     1801 ctggctcgtg ccgcggcgat gaatcccaca ctggaagata cgggctacca agtcgaagtt
     1861 taagcctgtg gtttacgaca ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg
     1921 tttttactct gtttcatcgg atttagcgtt aaaggaatag tccgtttccg ggcaacaata
     1981 agttgtaagc aaccaagcgc aggaatatgc gcattcaaaa gttttcctcc gtgtttgttc
     2041 catgggagaa cgtggaaacg gctggcgtgt agcacttcaa aattcaacaa agagagccag
     2101 tccagttgcc gcatcagagt aaacatccgg ctgttatagg gcgaggtttt gcgcaatacc
     2161 ggcacaagtt tgcgtaatcc cattaaacta atgggattga agccactaat gaccagccag
     2221 ccatcatcaa tcaatacccg atcggcttca cgcaataaac gatgcggatc ggtgcaccac
     2281 ggcaatgtat gtgccagtag acaaacatca acggatttat cggcaaaagg aagatgaagt
     2341 gggtccgcct gcacctggac aggcattcct tgcgcagaaa cattcacttg atgagaaacc
     2401 gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa tcttaagcag atgaaaacca
     2461 tacattttag tgaaccacgg gttgagctgg cgctccagcg ccttgcgata aagctctccc
     2521 cagggcaaat cgccccagcg atcaggagcc acgacagttt gagggactct tgccggtttc
     2581 atcacaacct tccgtttcac actgagaggt aatctatgaa tcttaacagt attcccgcct
     2641 ttgatgacaa ttacatctgg gttttgaatg atgaagcagg tcgctgcctg attgtcgatc
     2701 ccggagacgc agagccagta ttaaacgcca ttgccgccaa taactggcaa ccggaggcca
     2761 tatttctcac ccaccatcat cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt
     2821 ttccgcaaat tgtggtgtat ggtccacaag agacacaaga taagggaaca acacaggtag
     2881 tcaaagatgg cgaaactgcc ttcgttttag ggcatgaatt tagtgtaatt gccacaccgg
     2941 gtcacacttt aggacatatc tgttacttca gtaaacctta tctattttgc ggcgacacgc
     3001 tgttttctgg tgggtgtggt cggttgtttg aagggacacc atcacaaatg tatcaatcac
     3061 ttaaaaagtt aagtgcgtta cctgacgata cattggtatg ttgtgctcat gaatatacct
     3121 tatcaaatat gaagtttgct ttgagtattc ttccgcacga tttgtccata aatgattatt
     3181 atcgtaaagt taaggagtta cgggcaaaaa atcaaataac actacccgta attctgaaaa
     3241 atgagcggca aattaatgtt tttttaagaa cggaagatat tgatttaatt aatgtaatta
     3301 atgaagaaac attattgcaa caacctgaag agcgttttgc atggttaagg tcaaagaaag
     3361 ataggttctg ataaaacttt cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt
     3421 ttaagcaact attgacacac acatgaaggc aaaagcgata ttactcgcct ctgtcctgct
     3481 cgtgggttgc cagagtaccg gcaacgttca acagcacgca cagagccttt ctgcagctgg
     3541 tcaaggggaa gcagcaaagt ttacaagtca ggcacgatgg atggacgatg ggacgtctat
     3601 cgcgccagat ggtgacttgt gggctttcat tggcgacgag ctaaagatgg gaattccgga
     3661 aaatgaccgg attcgcgaac agaaacagaa atatttacgc aataagagct atctccacga
     3721 tgtaacttta cgggcagagc cgtatatgta ctggatagcc gggcaagtta aaaaacgtaa
     3781 catgcctatg gaactggtac tactacccat agtggagagc gcttttgatc ctcacgcaac
     3841 gtctggcgcc aatgccgcag gcatctggca gatcattccg agcacggggc gcaattatgg
     3901 tttgaaacag acccgcaatt atgacgcgcg tcgcgatgtt gttgcttcaa caactgccgc
     3961 gctgaatatg atgcagcgtc tgaacaaaat gtttgatggc gactggcttc tgaccgtagc
     4021 ggcttataac agcggcgaag gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg
     4081 gaaatccacg gacttctggt cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa
     4141 aatgctggca ttgagtgata ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac
     4201 gaccgatgaa agccgtgctc tggcgcgtgt gcacctgagt agcccggttg aaatggcgaa
     4261 ggttgcagat atggcgggga tttccgtcag caagctgaag acattcaacg ctggcgtgaa
     4321 aggctccacg ctgggcgcaa gtggtccgca gtacgtgatg gtgccaaaga agcatgcaga
     4381 tcaactgcgt gaatctctgg cttcaggcga aattgctgct gtacagtcga cgctgattgc
     4441 cgacaatacg ccgcttaaca gccgtgttta caccgtacgc tctggcgaca cgctttcaag
     4501 catcgcttca cgtctcggcg taagcaccaa agatttgcag cagcggaaca aactgcgcgg
     4561 ctctaagctg aaaccaggcc aaagtttgac gattggtgca ggcagtagcg cacagcgact
     4621 ggcaaacaac agcgatagca ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat
     4681 tgctaaacgc cacggcgtga acatcaaaga tgtgatgcgc tggaacagcg acactgcgaa
     4741 tctgcaacca ggcgataagc tgacgttgtt tgtgaaaaac aacaacatgc cagattcctg
     4801 acaaatcaga taatcaaaag gcaccgattc ccccggtgcc ttttttattt atgccgcttt
     4861 atgtgcatct accatgatga tatcactggt gaaagagcca tcattctgca aggcaaaata
     4921 cgttctcacc tctgtcgatg cgctctgttg gtaaatgcga atagcgtcta ctaacgcttc
     4981 tggcgtacgc attctcgcga cccatgaaga aaattccagc ggtaacttat ctgtaattaa
     5041 attatcaact atcagattgg cttcattgat taacgtcaac cactcaccgc tggcgtagtt
     5101 tcgtacgtga gaggtatcgc gtaatgcttc taccgtctgt aaccagatgt cgcgcactgg
     5161 gtgacccgga gacattacgt ccatcacaat cagcctaccg ccaggtttca atatccggtt
     5221 cacttcacgc agtgctgcac caacatcatg ccagtgatgg gcagaataac ggctgataac
     5281 aatatcaaat gcgttatcgg caaatggcag actttcggca tatccctggc gggtggtgat
     5341 atttttcagt tgccgggctt cggcagcttg tgccacgaca tccagcatat gggcagataa
     5401 gtcatacgcc accaccgtgc tcacgttttg cgcagcgaca aagctggcat gccctgctcc
     5461 gcagcccata tcaagtacac ttgcatcagg ataatcagcc agacgcaccg ccaggcgctg
     5521 taaatctcgt ccggatgcat gtacggtact ggtcagatat tcgctggctt gagagctaaa
     5581 ctgcttttct acgtggtcat ggtgggattg tgttgtcatt acgctgtcct tttgttgttt
     5641 gagtataaaa gggcagcgcc atacgttggt ctgccctgtg gcagacctga cataccttat
     5701 ttatcaggct tgccgggact gaattcaacg agtagcggat tgtggtcaga agcgcgcgta
     5761 accagtacag aagcttcgct gacgttcaga ccacggtaga aaacaaaatc gagcgggcga
     5821 ccaaacgccc gacggcgttg atcatcggta aaacgcacct ggcgcagcga catttcccgc
     5881 gcaaagcgat ataacgcgtt catccttcta cggctccagg cattgaaatc tcctgccata
     5941 atgaccgggc cgctgtggtg agctatctga tcaccaatag gaagtaactg cttactataa
     6001 acatccacgc ccagactgaa gttgacggcg tgtatattaa ccaccatcaa caggcgggtg
     6061 tcaggtaatg gatagaccgt caccagtgcc gacttcgcca gacgcaaaat gggctctcgt
     6121 tcacgtaacg ggcagcaata cactggatgc gccgccgaaa gggtcattac gccagaagga
     6181 tgttgtggca gcacgaaagc aggcacctga tcggcggcaa gatagttagc ggtcgcaaac
     6241 tgcaccaact ctggcgttgt ttgcgcttcc tgcaataaca ccagatgcgc atctttgccg
     6301 tagttcttta ataccgacaa ccattcagcg cgttgctgtt tgtaaatgtt ccacaccaag
     6361 atccgaatac gctcttctgt acttaacggt tctccgggtg gtaatgcctg gccgatgctc
     6421 gcaaaagacc ccggcggtaa gatcctttcc gcaggttgtc cggcaacata gcgcatggca
     6481 taggtgtttt ttcgcacttt tgactttgaa acctctatta cgtcaaccct tccgcactcg
     6541 ggagggttct actgttatag ggattttagc tcactctttc aacgagcaat tgtatattgt
     6601 tatgtaagca agtgcttaca ccggtcagaa ttttgctcat tattgagaac attaaatcgg
     6661 atgacatatc acgcatcctg atggtttaat gacaaataaa ttcaaaatat ctcttctatt
     6721 ttgcaacagg agcaaaaatg aaagccacgt cggaagaact cgccattttt gtttcggtcg
     6781 tagaaagcgg cagctttagc cgggcagcgg aacaattagg gcaagcaaac tctgcggtaa
     6841 gccgggcggt gaaaaagctg gagatgaaac ttggcgttag cctgcttaat cggaccacgc
     6901 gacaacttag cctgacggaa gaaggcgagc gttattttcg tcgcgtacag tcaattttgc
     6961 aggagatggc agcggcagaa tcagaaatta tggagacgcg taatacaccg cgtggactgt
     7021 tacggatcga tgccgcaact ccagtggtgc tgcactttct gatgccgtta attaagcctt
     7081 tccgtgaacg ctatccggaa gtcactttgt cgctagtctc ctccgaaacg attattaatt
     7141 tgatcgaaag aaaagtggat gtcgcgatac gcgctggtac gttaacggat tccagcttac
     7201 gtgccaggcc gttatttaac agttatcgaa aaattatcgc ctcccccaat tatatttccc
     7261 gctacgggaa gccagaaacg atcgacgatt taaagcaaca tgtttgcctg ggattcactg
     7321 aacccgcttc cctcaatacc tggccgatag cctgtagcga tggacaatta catgaggtga
     7381 agtacggttt gtcatccaat agtggggaaa cactgaaaca gctttgcctg agtggcaacg
     7441 ggattgcgtg tttgtccgac tatatgatcg acagagaaat cgctcgcgga gaattggtgg
     7501 agttaatggc agataaagtg ttgccagtgg aaatgccatt cagtgcagtc tattacagcg
     7561 accgtgcggt aagtacgcgc atccgggctt ttatcgattt ccttagcgag catgtaaaaa
     7621 cagctcccgg aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc
     7681 taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct
     7741 gtaaattttg tgccttaaga ttactttcca ggtttttacg tttagtagaa gaaggaatta
     7801 ctgagtaacc ttcccccata gcccacgcca gaatcacttg tgccggagtc gcattgtgtt
     7861 tagctgcgat acgagcaata acctcatctt tcagggcctt accatacgcc agcgtcatat
     7921 aggaagtaat atggatgccg tgctgtttag cccaggcaac cactttacgg ttttgcagat
     7981 aaggagagag ttcaatctgg ttagtagcga tgttttcagc gccaacagca gcaatcgcct
     8041 tttccatcaa tgggatcgtg aagttggaaa taccgatctc acgcgtcaac ccttcttttt
     8101 tggcttccag cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc
     8161 agtggattag agtcagatca acataatcag tacgcaattt ttgcaggctc tctttcagac
     8221 tcgggatcaa tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt
     8281 catgacgtga cacgccactt tctgcaatcg cctgacctac ggcagcttcg ttatcataga
     8341 tttgtgcggt atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa
     8401 caacgtcgtc tttcagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc
     8461 tcttttaatg ctattttcgt taactgaggt gattatgggc ttcagactgt tgcggaaaaa
     8521 gagcgtaaaa cgcagaggat ttttgcgatt ttagcaataa tagaaatgta gacgaaaaaa
     8581 aacctcgagc gataactcga ggcttcttaa taagtggcgg aacggacggg actcgaaccc
     8641 gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc
     8701 ttttacaaca accggtttta tgaccggctt actgct
//
LOCUS       DAZFZC010000154         8716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFZC010000154 DAZFZC010000000
VERSION     DAZFZC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.156"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            135..1265
                     /gene="ompC"
                     /locus_tag="ACHKXP_004614"
     CDS             135..1265
                     /gene="ompC"
                     /locus_tag="ACHKXP_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7649465.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYTSNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1377..2432
                     /gene="apbE"
                     /locus_tag="ACHKXP_004615"
     CDS             1377..2432
                     /gene="apbE"
                     /locus_tag="ACHKXP_004615"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO7649466.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2506..3570
                     /gene="ada"
                     /locus_tag="ACHKXP_004616"
     CDS             2506..3570
                     /gene="ada"
                     /locus_tag="ACHKXP_004616"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO7649467.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3570..4220
                     /gene="alkB"
                     /locus_tag="ACHKXP_004617"
     CDS             3570..4220
                     /gene="alkB"
                     /locus_tag="ACHKXP_004617"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO7649468.1"
                     /translation="MLDLFADGEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4296..5939
                     /gene="yojI"
                     /locus_tag="ACHKXP_004618"
     CDS             4296..5939
                     /gene="yojI"
                     /locus_tag="ACHKXP_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO7649469.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6157..7803
                     /gene="mqo"
                     /locus_tag="ACHKXP_004619"
     CDS             6157..7803
                     /gene="mqo"
                     /locus_tag="ACHKXP_004619"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO7649470.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKCLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
     gene            complement(8181..8690)
                     /gene="eco"
                     /locus_tag="ACHKXP_004620"
     CDS             complement(8181..8690)
                     /gene="eco"
                     /locus_tag="ACHKXP_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO7649471.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         2071 a         2183 c         2387 g         2075 t
ORIGIN      
        1 gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga
       61 ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac
      121 agagggttaa taacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag
      181 caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg
      241 gtaaagtaga cggcctgcac tatttctctg acaacaagtc agaagacggc gaccagacct
      301 atgtacgtct tggtttcaaa ggcgaaactc aggttactga ccagctgacc ggttacggcc
      361 agtgggaata tcagatccag ggcaatacct ctgaagacaa caaagaaaac tcctggaccc
      421 gtgtggcatt cgcaggtctg aaattccagg atgtaggttc tttcgactac ggtcgtaact
      481 acggcgttgt ttacgatgta acttcctgga ccgacgtact gccagaattc ggtggcgaca
      541 cctacggttc tgacaacttc atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca
      601 ccgacttctt cggtctggtt gacggtctga actttgctgt tcagtaccag ggcaaaaacg
      661 gtagcgtaag cggcgaaggc atgaccaaca atggtcgtgg tgctctgcgt cagaatggcg
      721 acggtgtcgg cggatctatc acttatgatt acgaaggctt cggtatcggt gctgcagttt
      781 ccagctccaa acgtactgat gatcaaaatg gtagctacac cagcaatggt gtagttcgta
      841 actacatcgg tactggcgac cgtgctgaaa cctacactgg tggtctgaaa tacgacgcta
      901 acaacatcta cctggctgct cagtacaccc agacctacaa cgcaactcgc gtaggttccc
      961 tgggttgggc gaacaaagca cagaacttcg aagctgttgc tcagtaccag ttcgactttg
     1021 gtctgcgtcc gtccctggct tacctgcagt ctaaaggtaa aaacctgggt gtcatcaatg
     1081 gtcgtaacta cgacgacgaa gatatcctga aatatgttga tgttggtgcg acctactact
     1141 tcaacaaaaa catgtccacc tacgttgact acaaaatcaa cctgctggac gacaaccagt
     1201 tcactcgtga cgctggcatc aacactgata acatcgtagc tctgggtctg gtttaccagt
     1261 tctaatctcg attgatatcg aacaaagggc ctgcgggccc ttttttcatt gttttcagcg
     1321 tacaaacaca gttttttggt gtactcttgc gaccgttcgc atgaggataa tcacgtatgg
     1381 aaataagctt tacccgcgtg gcactgctgg ctgccgcgct cttctttgtt ggttgcgatc
     1441 aaaaaccaca acccgccaaa acccacgcta ctgaagttac cgttcttgaa ggcaaaacca
     1501 tgggtacctt ctggcgtgcc agcatcccgg gcattgatgc caaacgcagt gccgaactta
     1561 aagaaaagat tcagacccag ctggacgccg acgatcagct gctttcgacc tataaaaaag
     1621 attccgcgct gatgcgcttt aacgactcgc aaagtttgtc gccgtggccg gtaagtgaag
     1681 cgatggccga tatcgtcacc acctcgctgc gcattggcgc aaggaccgat ggtgcgatgg
     1741 atataaccgt cgggccactg gtgaatctgt ggggttttgg cccggaacaa cagccggtac
     1801 aaatcccgag ccaggaacag attgatgcga tgaaagccaa aaccggctta cagcacctga
     1861 cggtcattaa tcagtcgcat cagcaatatc tgcaaaaaga cctgccggat ttatatgtcg
     1921 atctctccac cgtcggtgaa ggttatgcgg cggatcacct ggcacgcttg atggagcagg
     1981 aagggatttc ccgctatctg gtgtcggtgg gcggcgcgct gaacagccgt ggtatgaacg
     2041 gtgaaggcca gccgtggcgg gtagcgattc aaaaaccaac cgataaagaa aacgcggttc
     2101 aggctgtggt ggatatcaac ggtcatggga ttagcacctc tggcagctac cgtaactatt
     2161 acgaactgga cggcaaacgt ctttcccatg ttatcgatcc gcaaaccggg cgtcccatcg
     2221 aacacaatct ggtatccgtg acggtgattg ctccgacggc gctggaagcc gatgcctggg
     2281 atactggctt gatggtactc gggccggaga aagccaaaga ggttgttcgc cgggaagggc
     2341 tggcggttta tatgatcacc aaagaaggcg atagctttaa aacctggatg tcaccacagt
     2401 ttaaaagctt ccttgtcagc gaaaaaaatt aaagcgcaag attgttggtt tttgcgtgat
     2461 ggtgaccggg cagactaaag gctatcctta accagggagc tgattatgaa aaaagccaca
     2521 tgcttaactg acgatcaacg ctggcaatct gtcttagccc gcgacccgaa tgccgacggc
     2581 gaattcgttt tcgccgtgcg taccacaggc atcttttgcc gtccgtcttg ccgcgccaga
     2641 catgctttgc gggaaaacgt ctccttctac gcaaatgcca gtgaggcact cgccgctggc
     2701 tttcgcccct gcaaacgttg tcagccagac aaagccaatc cccggcaaca tcggttggat
     2761 aaaatcaccc acgcgtgtcg actgctggaa caggaaacgc ctgtaacgct ggaagcctta
     2821 gccgaccagg tggcgatgag tccatttcat ctacatcggt tgtttaaagc gactaccgga
     2881 atgacgccta aagcctggca acaggcctgg cgcgctcgcc gtttgcgcga atcgctggcg
     2941 aaaggggaga gcgtgacgac gtctattctt aacgccggat tccccgacag cagcagttac
     3001 taccgcaaag ccgatgaaac gctgggcatg acggctaaac aattccgtca cggtggcgaa
     3061 aatctggcgg tgcgttacgc gctggctgat tgtgagctgg gtcgttgcct ggtggcagaa
     3121 agcgagcggg ggatttgcgc gatattgctg ggcgatgatg acgccacatt aatcagcgag
     3181 ttgcaacaaa tgtttcccgc tgccgacaac gcgcctgccg atctgatgtt tcagcaacat
     3241 gtgcgtgaag tgatcgccag cctcaatcaa cgcgatacgc cgctgacgtt accgctggac
     3301 attcgcggca ctgcttttca gcaacaagtc tggcaggcac tgcgcacgat accttgcggt
     3361 gaaaccgtca gttatcagca actggctaac gccatcggca aaccgaaagc ggtacgggcc
     3421 gttgcaagtg cctgtgccgc caacaagctg gctatcgtta taccctgtca tcgggtagta
     3481 cgcggtgatg gatcgctttc aggttaccgc tggggcgtgt cgcgtaaagc gcaactgctg
     3541 cgccgcgaag ctgaaaatga ggagaggtaa tgttggatct gtttgccgat ggtgaaccgt
     3601 ggcaagagcc actggcggct ggagcggtaa ttttacggcg ttttgctttt aacgctgcgg
     3661 agcaactaat ccgcgatatt aatgacgttg ccagccagtc gccgtttcgc cagatggtca
     3721 cccccggggg atataccatg tcggtggcga tgaccaactg tgggcatctg ggctggacga
     3781 gccatcggca aggttatctc tattcgccca ttgatccgca aacaaataaa ccgtggcccg
     3841 ccatgccaca gagttttcat aatttatgtc aacgtgcggc tacggcggcg ggctatccag
     3901 atttccagcc agatgcttgt cttatcaacc gctacgctcc tggcgcgaaa ctgtcgctgc
     3961 atcaggataa agacgaaccg gatctgcgcg cgccaattgt ttctgtttct ctgggcttac
     4021 ccgcgatttt tcaatttggc ggcctgaaac gtaatgatcc gctcaaacgt ttgttgttgg
     4081 aacatggcga tgtggtggta tggggcggtg aatcgcggct gttttatcac ggtattcaac
     4141 cgttgaaagc ggggtttcat ccactcacca ccgactgccg ctacaacctg acattccgtc
     4201 aggcaggtaa aaaagaataa aaataagaat tattattgct gtgcgcgcga agatgtttaa
     4261 actgcgggct gtaattcatt gtccgggttt tctgcatgga acttcttgta cttgtctggc
     4321 ggcagtatcg ctggccattt atcagtgtga tggcgctaag cctcgccagt gcggcattag
     4381 gcattggctt aattgctttt atcaatcagc gccttatcga aacggcggat accagtctgc
     4441 tggtgttgcc ggagtttctt ggattattac tgctgttgat ggcagtcact ctcggatcgc
     4501 aactggcgct caccactttg gggcatcact tcgtttaccg actgcgtagt gaatttatca
     4561 agcggattct ggatacccac gtcgagcgca ttgaacaact cggtagcgcc tcgttgctgg
     4621 cgggattaac cagcgatgtg cgcaatatca ccattgcttt tgtgcgtctt ccggaactgg
     4681 tgcaggggat cattctcact atcggttccg cggcgtatct gtggatgctg tcgggcaaaa
     4741 tgttgctggt aacggctatc tggatggcga tcaccatctg gggtggtttt gtactggtgg
     4801 cgcgggtgta caaacatatg gcgaccctgc gtgaaaccga agacaagctg tacacggatt
     4861 ttcaaaccgt actcgaaggg cgcaaagagc tgactctgaa ccgggaacgc gccgagtatg
     4921 tgtttaacaa cctctacatc cccgatgcgc aagagtatcg ccatcatatt attcgcgcag
     4981 acaccttcca tcttagcgcc gtgaactggt caaacatcat gatgctgggc gctatcggcc
     5041 tggtgttctg gatggcgaac agtctcggtt gggctgatac caacgttgct gcgacctatt
     5101 cgttgacgct gttattcctg cgtacgccgc tgctttcggc ggttggcgca ttgccgacgc
     5161 tgctgacggc gcaggtggcg tttaacaagc tgaacaaatt cgcgctcgcg cctttcaaag
     5221 ctgagtttcc gcgcccgcag gcgtttccca actggcaaac gctggagctg cgtaacgtga
     5281 cgtttagcta tcaggataac gcgttttccg ttgggccgat taacctcacc attaaacgtg
     5341 gcgagctgct gtttctgatt ggtggcaacg gtagtggaaa atcgacgctg gcgatgttgc
     5401 tgacgggctt gtaccagcca caaagcggcg aaatcttgct ggatggcaaa cctgtcagtg
     5461 gcgaacaacc ggaagattat cgcaaactgt tttcggcagt gtttaccgat gtctggctgt
     5521 ttgatcaact gctggggccg gagggtaaac ccgctaaccc gcaactggtt gagaagtggc
     5581 tggcgcagct gaaaatggct cataagcttg agttaagcaa cgggcgtatt gttaacctga
     5641 agttatcaaa agggcagaaa aaacgcgtgg cgctgttgct ggcgctggca gaagaacgcg
     5701 atattatcct gctggatgaa tgggcggcgg atcaggatcc acacttccgt cgtgagtttt
     5761 atcaggtgtt gctgccgctg atgcaggaga tgggtaaaac tattttcgct atcagtcatg
     5821 atgatcatta ctttatccac gccgaccgcc tgctggaaat gcgcaatggg caacttagcg
     5881 agctgacggg cgaagagcgc gatgccgctt cgcgtgatgc cgttgcccgg acggcataat
     5941 attacgacgc tgacctggcg gactgctgcc gtcaggtcaa tatgctaaaa atcccttcat
     6001 ttctaataac cctataatta atcaacgaaa ttataatgtt tctaaaatta gaatataatt
     6061 tataaacatt atttaaatgt tgttacttaa gtgttaccgt tgatgccgcg caaatctcac
     6121 ctgcaataaa gcgactaaaa gtaaggcatt aacaagatga aaaaagtgac tgccatgctc
     6181 ttctcgatgg ccgtggggct taatgccgtt tcgatggcgg caaaagcgaa agcgtctgag
     6241 gagcaggaaa ctgatgtact gttgattggc ggcggcatta tgagcgccac gttggggacc
     6301 tatttacgcg agctggagcc tgaatggtcg atgaccatgg tggagcgcct ggagggtgtc
     6361 gcgcaggaga gttcgaacgg ctggaataac gccggaaccg ggcattctgc actgatggaa
     6421 ctgaactaca ccccgcaaaa cgccgatggc agcatcagta ttgaaaaagc agtcgccatt
     6481 aacgaagcat ttcagatttc ccgccagttc tgggcgcacc aggttgagcg cggcgtgctg
     6541 cgtactccgc gttcatttat caataccgtt ccgcatatga gctttgtctg gggcgaggat
     6601 aacgttaatt tcctgcgcgc ccgttacgcc gcgttgcaac aaagctcgct gtttcgcggt
     6661 atgcgttact ctgaagatca cgcgcagatc aaagagtggg caccgttagt gatggaaggg
     6721 cgcgatccgc aacagaaagt ggcagccacg cgtacggaaa ttggtaccga tgtgaactac
     6781 ggcgagatca cccgccagtt aattgcttcc ttgcagaaga aatctaactt ctcgctgcaa
     6841 ctcagcagcg aagtccgcgc cctaaagcgt aatgacgata acacctggac cgttaccgtt
     6901 gccgatctga aaaatggcac tgcacagaac attcgtgcca aatttgtctt tatcggcgcg
     6961 ggcggtgcgg cgctgaagct gttacaggaa tcggggattc cggaagcgaa agactacgcc
     7021 ggtttcccgg tgggcggaca gttccttgtt tcggaaaacc cggacgtggt taatcaccat
     7081 ctggcgaagg tttacggtaa agcatccgtt ggcgcaccac cgatgtcggt tccgcatatc
     7141 gatacccgcg ttctggacgg taaacgcgta gtgctgtttg ggccatttgc caccttctca
     7201 accaaatgcc tcaaaaacgg ttcattgtgg gatctgatga gttccaccac cacctctaac
     7261 gtgatgccga tgatgcacgt cgggctggat aatttcgatc tggtgaaata tctggtgagt
     7321 caggtgatgt tgagtgaaga ggatcgtttt gaagcgttga aagagtacta tccgcaggcg
     7381 aaaaaagagg actggcgttt gtggcaagcg gggcagcgcg tgcagattat caagcgtgat
     7441 gccgagaaag gtggcgtact gcgtctgggt actgaagtcg tcagtgacca gcaagggacc
     7501 attgccgcgc tcctgggggc atcgccaggg gcgtcaaccg ccgcgccgat tatgttggat
     7561 ctgctggaaa aagtatttgg cgatcgcgtt tccagcccgc aatggcaggc tacgttgaaa
     7621 gcgatcgttc cgtcttatgg acgcaagctg aacggtgatg tagcggcaac agaacgcgag
     7681 ttgcagtaca ccagcgaagt gctggggctg aaatacgaca agccgcaggc agcagatagt
     7741 acgccgaaac cgcagttgaa accgaaaccc gttcaaaaag aagtggcgga tattgcgttg
     7801 taatgatgtg ccacatccgg catggtatgc cggggatgtg gcgtatgctg ataagacgcg
     7861 ccagcgtctt atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     7921 atccaatcta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     7981 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtaaccgc cttatccaac
     8041 ctacggggtg gagcacttca cagcggtgta ggcctgataa gacgcgccag cgtcgcatca
     8101 ggcaaccggc tcgggcgttg gatgtcggat gcggcgtgac cgccttgtct gacctacggg
     8161 gtgccgcact tcacggcagt ttagcgaact accgcgttgt caattttctc ttccgccttc
     8221 cagacgcggt acttcacatc tacattgtct ggcgtataca ccacgatcgg cagcttgctg
     8281 ttgtaacgca gcattccagc atcgcccaga tacgcggtga caaatttctt ctctttcttg
     8341 ccatctgggc aggccatcat cgtcgaaacc ggggaactga ctttatcaaa gacataataa
     8401 tcatagcccc agccttccag cgttttgttt tccagcttcc cgccgagacg atgcaaattg
     8461 caatcgactt ccagcgtctg accgattaac agttctactt tcagggtaga ttcatcttct
     8521 tgcggggtta actgaatcac ctgacgcttc atcccttttt cagcttgtgg ataaggcgcg
     8581 attttttcca gtggctggac gctttctgcc gcccaggcgg aagtggtagc gaaagcggca
     8641 aacaatactg caggtagaat ggtcttcatt tttttgcttt ccttcttcat ttataggttt
     8701 tcaccgatga tgtcgc
//
LOCUS       DAZFZC010000155         8680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFZC010000155 DAZFZC010000000
VERSION     DAZFZC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.157"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(106..726)
                     /gene="entD"
                     /locus_tag="ACHKXP_004621"
     CDS             complement(106..726)
                     /gene="entD"
                     /locus_tag="ACHKXP_004621"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO7649472.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(901..3141)
                     /gene="fepA"
                     /locus_tag="ACHKXP_004622"
     CDS             complement(901..3141)
                     /gene="fepA"
                     /locus_tag="ACHKXP_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO7649473.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3384..4586
                     /gene="fes"
                     /locus_tag="ACHKXP_004623"
     CDS             3384..4586
                     /gene="fes"
                     /locus_tag="ACHKXP_004623"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO7649474.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWKTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4589..4807
                     /gene="ybdZ"
                     /locus_tag="ACHKXP_004624"
     CDS             4589..4807
                     /gene="ybdZ"
                     /locus_tag="ACHKXP_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO7649475.1"
                     /translation="MAFSNPFDDPQGAFYILRNGQGQFSLWPQQCVLPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            4804..>8680
                     /gene="entF"
                     /locus_tag="ACHKXP_004625"
     CDS             4804..>8680
                     /gene="entF"
                     /locus_tag="ACHKXP_004625"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO7649476.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQLNATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCEVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGTFEKIGPIIRATLN"
BASE COUNT         1878 a         2348 c         2390 g         2064 t
ORIGIN      
        1 aatccccgca tatccggttg tcaggtcagg atggtaaggc acctgcttta cactttcgcc
       61 cgtggtcagt gatggctgcg ggcgaatcgt accagatgtt gtcaattaat cgtgttggca
      121 cagcgttatg actatctttt cttttatctg ccagtgcaca gcaaacatct cattctcacg
      181 atgaatgatg acctgctgtt tattccagct aattatctga tagtccagaa aacctgcatc
      241 agtttggatc tcacttgcct taaatgcgct ctctttggcg gaaaatgcca gtgtcagcgc
      301 cagagaaaag gctaaaccgc agtctgcgag tcgctcgtgt tctgctggtg taataatgtt
      361 gtctgtcaat tcggtcgctg tttgcgcaga aaaaatttct tcgatatcaa cgccaatcgg
      421 ttgacgggaa accacggcca atgccgtagc cccgcagtgg ctaatactgc cgtatacctc
      481 cgcaggccac accggttggc gtagctcgcc gattgcgggc acacatttat agccatattc
      541 ccgcaaagca taaacagcag cgatccgtcc ggctaaatgc tctgttttac gtttacgtcc
      601 agcgtgttgc agttgtgcgt agtgcggcag ccagagtaaa tcctgctcac aaaaattcgc
      661 cggatcgaac tcaacaaaat gcagcgtatg tccggcaaag gggagggagg tatgcgtagt
      721 tttcatatcg accatattcg agactgatga caaacgcaaa actgcctgat gcgctacgct
      781 tatcaggcct acatggtcct gcaatatatt gaattggcaa gattttggta ggccggataa
      841 ggcgttcacg ccgcatccgg catgaacgac gcgcactttg tcaacaatct gacgttagca
      901 tcagaagtga gtgtttacgc tcatatacca cgtacgtccc ggctcgttat aggtatacgc
      961 cccggcaccg gcgatatagt tggcccctgc caaatcgccc gtggtctggg cattacccgc
     1021 acgccacaaa cgtttgtcga acagattgtc cacgccgccg gtcagactga cattcttcgt
     1081 cacatcccag gtcgcgctca ggccaacaat gctgtaagga ctaatttctt tggtttccgg
     1141 tccaaccgct ggctgacctt tatagttgta cttcttcggc tgctgcttgc cgtaccaggt
     1201 gaaggtcgtt tgcatcgaca aatcttcccg tgcctgccag ctcagcgttg agttcaacgt
     1261 atactccggg atgatcgaca aacggtcgcc cgtggttttg ttttcactct tcagcatata
     1321 agtgatgtta ttggtccaca tcaccgtttc gctaaccggt acgtttaacg atccttccag
     1381 accttcaacc accgctttcg gcacgttatc ccactgatag agatcggtgc cgactgcgtt
     1441 ttgccctaca gccacatagc ccgcttcaat cttattgcga taatcgttac ggaaccaggt
     1501 gacgcccgcc agccagccgt cgcgtttgaa ctccagacca atctctttgt tgatgctggt
     1561 ttctgctttc aggtcatcgt taccttgcag atagcagccg cccgcgctgg catagcaacc
     1621 ctgaccttta ctgtagagaa tgtagttcgg gttagtctgg tacaggctcg gcgctttata
     1681 agcacgggcg atgcccattt tcagcgtgaa gtcatcgcct aaaccttgcg atatgttcag
     1741 cgccgggctc cagttattgc cgacaatact gtgatgatcg aaacgcagcc ccggcgttac
     1801 gatggtgctg tcagtcagct ccatgttgtt ttcggcaaac agcgagaaaa tttctgcttt
     1861 tgaatacggg ctacggtcgg tggcactcac gccatcaata gcgccaccgg tattcgttcc
     1921 ggtcagtgcc tgggtgttgg aacttaagtc cttcatccgt tgctgattcc actccgtacc
     1981 cagcgtcagc gtctggttaa cgaggaaatc aatcggcagg ttaacttcgc tgtgcagcat
     2041 cacgtcatca agatcgatat cgacgaaatc ctgtgtcgct ttttcgttaa atttcccttc
     2101 ggtaccgccc gccagacctt ccggaatacg cgagttacgg gtgtgttcgt actgcaccca
     2161 gttgctggtg gtcacgccgt tatcccagcc accgttccag gtcagcgcgt agttctggcg
     2221 atacagacgg ttggtttcat cgccatattt cgagcgggta taggaatcgg agttggtatt
     2281 ctgggtgtcg cccgcataca ggttaccctg gcggctgtaa cctgcttcca gttccagcga
     2341 ttgcaatggc gcgaaatccc agcgcaccac gccattaata tctttgttga ttaccccttc
     2401 gcgcccggct ggtaacgtcg tggcatacgt tccggcacgc gcggactgat ggccctggtt
     2461 gatatcccac gcgtcagcct gggttttgtc gaggttgcca tacaaacgga agctgaattc
     2521 gtcgcccagc ggaccggtca ggctaaagtt agtgcgtttg gtggcacctt cctctttatg
     2581 ttctggcgca ttgaaatatg cgtcccagga gccgtgccac tcgccgctgc cttttttggt
     2641 aatgatgtta accacgccgc ccgccgcgcc gttgccataa cgcgcagctg ccggaccacg
     2701 cagaacttca atacgttcaa tcatttcagg tggcacccag gaagtatcac cacgggtatc
     2761 gcgctcgcca cgccagccct gacgcaccga gttacggctg cttaccggct tgccgtcaat
     2821 caaaatcagc gtgttttccg gacccatacc gcgaatatca atctgtcggt tattcccacg
     2881 ctgaccactg gtggagttac cggtcaggtt aacgcctggc atggtacgga tgatctccga
     2941 cacatcgcgg gcaaccgggt ttttgcggat ttcatctgcg gtgatggtcg aaacgccagg
     3001 cgcctgtaag ttctgctcgg cggcggtaac gacaatagta tcgtcatgtg aaacaggagt
     3061 atcggtcggc tcttgtgcct gcgctacccc ataaatcccc agattgacca acaaggccag
     3121 ggaatgaatc ttcttgttca ttgttttatt cctgcatttt tgccacgaat tgcaactgtc
     3181 gggcatggtc gtcatcaaca cgacgcatcc cgctaccgcg aaaacctttg atcctgaaag
     3241 acacgcagtg cagttggtta attaatgtcc gcgcttccca cggcgcgcca ttacgctatt
     3301 gcaaatgcaa atagttatca ataatattat caatatattt ctgcaatcaa tgaaaaattg
     3361 cacagtaaac atggggttat ggtgtgacgg cgttaaaagt aggaagtgag agctggtggc
     3421 agtcgaaaca tggcccggaa tggcagcgtc tgaatgacga aatgtttgag gtcactttct
     3481 ggtggcgtga tccccaaggt tctgaagaat actcgacgat aaagcgcgta tgggtctaca
     3541 tcactggtgt gaccgatcac catcagaaca gccagcccca gtcgatgcag cgaattgcag
     3601 gcactaacgt ctggcagtgg aagacacaac tcaatgccaa ctggcgcggc agctactgct
     3661 ttattcccac cgaacgcgat gacatttttt ctgtaccatc ccccgatcgc ctcgaattgc
     3721 gcgaaggctg gcgaaaacta ttaccccagg cgatagccga tccgctgaac ctacaaagct
     3781 ggaaaggcgg gcgagggcac gctgtttctg cactcgaaat gccgcaagcg cctctgcaac
     3841 cgggatggga ttgtccgcaa gcgccagaaa tacctgccaa agaaattatc tggaaaagtg
     3901 aacggttgaa aaagtcacgg cgtgtatgga tttttaccac cggcgatgca acagcagaag
     3961 aacgcccgct ggcagttttg ctcgatggcg aattttgggc gcaaagtatg cccgtctggc
     4021 cagtgctgac ttcgctgacc catcgtcagc aacttcctcc cgccgtgtat gtgttgatcg
     4081 acgctatcga caccacgcac cgcgcccacg aactgccgtg taatgcggat ttctggctcg
     4141 cagtacagca agagttatta cccctggtga aagctattgc cccttttagc gatcgtgccg
     4201 atcgcaccgt ggttgccggg cagagttttg gtgggctttc cgcgctgtat gccggactgc
     4261 actggcctga acgctttggc tgtgtattaa gccagtcagg atcgtactgg tggccgcatc
     4321 ggggcgggca gcaagagggc gtgttacttg aaaagctaaa agctggtgaa gttagcgccg
     4381 aaggtctgcg cattgtgctg gaagcgggta ttcgcgagcc gatgatcatg cgggccaatc
     4441 aggcgctgta tgcgcaatta caccccataa aagaatccat tttctggcgt caggttgacg
     4501 gcggacatga tgcgctttgt tggcgcggtg gcttgatgca ggggctaatc gacctctggc
     4561 aaccactttt ccatgacagg agttgaatat ggcattcagt aatcccttcg atgatccgca
     4621 gggagcgttt tacatattgc gcaatgggca ggggcaattc agtctgtggc cgcaacaatg
     4681 cgtcttaccg gcaggctggg acattgtgtg tcagccgcag tcacaggcgt cctgccagca
     4741 gtggctggaa gcccactggc gtactctgac accgacgaat tttacccagt tgcaggaggc
     4801 acaatgagcc agcatttacc tttggtcgcc gcacagcccg gcatctggat ggcagaaaaa
     4861 ctgtcagaat taccctccgc ctggagcgtg gcgcattacg ttgagttaac cggagaggtt
     4921 gattcgccat tactggcccg cgcggtggtt gccggactag cgcaagcaga tacgctgcgg
     4981 atgcgtttta cggaagataa cggcgaagtc tggcagtggg tcgatgatgc gctgacgttc
     5041 gaactgccag aaattatcga cctacgaacc aacattgatc cgcacggtac tgcgcaggca
     5101 ttaatgcagg cggatttgca acaagatctg cgcgtcgata gcggtaaacc actggtcttt
     5161 catcagctga tacaggtggc ggataaccgc tggtactggt atcagcgtta tcaccatttg
     5221 ctggtcgatg gctttagttt cccggccatt acccgccaga tcgccaatat ttactgcaca
     5281 tggctgcgtg gcgaaccaac gcctgcttcg ccatttacgc ctttcgctga tgtagtggaa
     5341 gagtaccagc aataccgcga aagcgaagcc tggcagcgtg atgcggcatt ctgggcagaa
     5401 cagcgtcgtc aactgccgcc gcccgcgtca ctttctccgg cacctttacc ggggcgcagc
     5461 gcctcggcag atattctgcg cctgaaactg gaatttaccg acggggaatt ccgccagctg
     5521 gctacgcaac tttcaggtgt gcagcgtacc gatttagccc ttgcgctggc agccttgtgg
     5581 ctggggcgat tgtgcaatcg tatggactac gccgccggat ttatctttat gcgtcgactg
     5641 ggctcggcgg cgctgacggc taccggaccc gtgctcaacg ttttgccgtt gggtattcac
     5701 attgcggcgc aagaaacgct gccggaactg gcaacccgac tggcagcaca actgaaaaaa
     5761 atgcgtcgtc atcaacgtta cgatgccgaa caaattgtcc gtgacagcgg gcgagcggca
     5821 ggtgatgaac cgctgtttgg tccggtactc aatatcaagg tatttgatta ccaactggat
     5881 attcctgatg ttcaggcgca aacccatacc ctggcaaccg gtccggttaa tgaccttgaa
     5941 ctggccctgt tcccggatgt acacggtgat ttgagtattg agatcctcgc caataaacag
     6001 cgttacgatg agccaacgtt aatccagcat gctgaacgcc tgaaaatgct gattgcccag
     6061 ttcgccgcgg atccggcgct gttgtgcggc gatgtcgata ttatgctgcc aggtgagtat
     6121 gcgcagctgg cgcagctcaa cgccactcag gttgagattc cagaaaccac gcttagcgcg
     6181 ctggtggcag aacaagcggc aaaaacaccg gatgctccgg cgctggcaga tgcgcgttac
     6241 ctgttcagct atcgggaaat gcgcgagcag gtggtggcgc tggcgaatct gctgcgtgag
     6301 cgcggcgtta aaccagggga cagcgtggcg gtggcactac cgcgctcggt ctttttgacc
     6361 ctggcactcc atgcgatagt tgaagctgga gcggcctggc taccgctgga taccggctat
     6421 ccggacgatc gcctgaaaat gatgctggaa gatgcgcgtc cgtcgctgtt aattaccacc
     6481 gacgatcaac tgccgcgctt tagcgatgtt cccaatttaa caagcctttg ctataacgcc
     6541 ccgcttacac cgcagggcag tgcgccgctg caactttcac aaccgcatca cacggcttat
     6601 atcatcttta cctctggctc caccggcagg ccgaaagggg taatggtcgg gcagacggct
     6661 atcgtcaacc gcctgctttg gatgcaaaat cattatccgc ttacaggcga agatgtcgtt
     6721 gcccaaaaaa cgccgtgcag ttttgatgtc tcggtgtggg agtttttctg gccgtttatc
     6781 gcaggggcaa aactggtgat ggctgaaccg gaagcgcacc gcgacccgct cgctatgcag
     6841 caattctttg ccgaatatgg cgtaacgacc acgcactttg tgccgtcgat gctggcggca
     6901 tttgttgcct cgctgacgcc gcaaaccgct cgccagagtt gcgcgacgtt gaaacaggtt
     6961 ttctgtagtg gtgaggcctt accggctgat ttatgccgcg aatggcaaca gttaactggc
     7021 gcgccgttgc ataatctata tggcccgacg gaagcggcgg tagatgtcag ctggtatccg
     7081 gcttttggcg aggaactggc acaggtgcgc ggcagcagtg tgccgattgg ttatccggta
     7141 tggaatacgg gtctgcgtat tcttgatgcg atgatgcatc cggtgccgcc gggtgtggcg
     7201 ggtgatctct atctcactgg cattcaactg gcgcagggct atctcggacg ccccgatctg
     7261 accgccagcc gctttattgc cgatcctttt gccccaggtg aacggatgta ccgtaccgga
     7321 gacgttgccc gctggctgga taacggcgcg gtggagtacc tcgggcgcag tgatgatcag
     7381 ctaaaaattc gcgggcagcg tatcgaactg ggcgaaatcg atcgcgtgat gcaggcgctg
     7441 ccggatgtcg aacaagccgt tacccacgcc tgtgtgatta accaggcggc tgccaccggt
     7501 ggtgatgcgc gtcaattggt gggctatctg gtgtcgcaat cgggcctgcc gttggatacc
     7561 agcgcattgc aggcgcagct tcgtgaaaca ttgccaccac atatggtacc ggtggttctg
     7621 ctgcaacttc cacagttacc acttagcgcc aacggcaagc tggatcgcaa agccttgccg
     7681 ttgcctgaac tgaaggcaca agcgccaggg cgtgcgccga aagcgggcag tgaaacgatt
     7741 atcgccgcgg cattctcgtc gttgctgggg tgtgaagtgc aggatgccga tgctgatttc
     7801 ttcgcgcttg gcggtcattc gctactggca atgaaactgg cagcgcagtt aagtcggcag
     7861 gttgcccgcc aggtgacgcc ggggcaagtg atggtcgcgt caactgtcgc caaactggca
     7921 acgattattg atgctgaaga agacagcacc cggcgtatgg gattcgaaac cattctgccg
     7981 ttgcgtgaag gtaatggccc gacgctgttt tgtttccatc ctgcgtccgg ttttgcctgg
     8041 cagttcagcg tgctctcgcg ttatctcgat ccacaatggt cgattatcgg cattcagtca
     8101 ccgcgcccca atggccccat gcagacggcg gcaaacctgg atgaagtctg cgaagcgcat
     8161 ctggcaacgt tacttgaaca acaaccgcat ggcccttatt acctgctggg gtattccctt
     8221 ggcggtacgc tggcgcaggg tattgcggcg cgactgcgtg cccgtggcga acaggtggca
     8281 tttcttggct tgctggatac ctggccgcca gaaacgcaaa actggcagga aaaagaagct
     8341 aatggtctgg acccggaagt gctggcggag attaaccgcg aacgcgaggc cttcctggcg
     8401 gcacagcagg gaagtacttc aacggagttg tttaccacca ttgaaggcaa ctacgctgat
     8461 gctgtgcgcc tgctgacgac tgctcatagc gtaccgtttg atggtaaagc gacgctgttt
     8521 gttgctgaac gcacgcttca ggaaggtatg agccccgaac gcgcctggtc gccgtggata
     8581 gccgagctgg atatctatcg tcaggattgt gcgcatgtgg atattatctc tccagggacg
     8641 tttgaaaaaa ttgggccgat tattcgcgca acgctaaaca
//
LOCUS       DAZFZC010000156         8645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFZC010000156 DAZFZC010000000
VERSION     DAZFZC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.158"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1158
                     /locus_tag="ACHKXP_004626"
     CDS             <1..1158
                     /locus_tag="ACHKXP_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO7649477.1"
                     /translation="RPLTFESNFTHGHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAF
                     HQSHTSFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVV
                     ARAIHKLSGRRNKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTG
                     TLFLDEIGDMPLMLQAKLLRAIEAREILPIGASSPIQVDIRIISATNQNLAQFIAEGK
                     FREDLFYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEILRK
                     HRWPGNLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLD
                     DAGGTALEEMEKQMIREALSRHNSKKQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1198..1677)
                     /gene="xdhC"
                     /locus_tag="ACHKXP_004627"
     CDS             complement(1198..1677)
                     /gene="xdhC"
                     /locus_tag="ACHKXP_004627"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO7649478.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(1674..2552)
                     /gene="xdhB"
                     /locus_tag="ACHKXP_004628"
     CDS             complement(1674..2552)
                     /gene="xdhB"
                     /locus_tag="ACHKXP_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO7649479.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(2563..4860)
                     /gene="xdhA"
                     /locus_tag="ACHKXP_004629"
     CDS             complement(2563..4860)
                     /gene="xdhA"
                     /locus_tag="ACHKXP_004629"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO7649480.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSQITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5275..6030
                     /gene="actS"
                     /locus_tag="ACHKXP_004630"
     CDS             5275..6030
                     /gene="actS"
                     /locus_tag="ACHKXP_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO7649481.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6109..6182
                     /locus_tag="ACHKXP_004631"
     tRNA            6109..6182
                     /locus_tag="ACHKXP_004631"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6141..6143,aa:Gly,seq:ccc)
     gene            6416..8026
                     /locus_tag="ACHKXP_004632"
     CDS             6416..8026
                     /locus_tag="ACHKXP_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO7649482.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSTLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            8039..8158
                     /locus_tag="ACHKXP_004633"
                     /pseudo
     CDS             8039..8158
                     /locus_tag="ACHKXP_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            8118..8645
                     /locus_tag="ACHKXP_004634"
                     /pseudo
     CDS             8118..8645
                     /locus_tag="ACHKXP_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         2343 a         1975 c         2138 g         2189 t
ORIGIN      
        1 agaccattaa catttgagag taattttacc catggacata tgcagcatat tgtttcatgg
       61 gacgataaaa gtgaattaat cattggtcaa ttgcataaca ttcagggccg acaattattt
      121 ttaatggcgt ttcaccaatc gcataccagt ttttctgtag caaatgcacc tgatgaaccg
      181 catattgaac aattggttgg cgagtgccgt gttatgcggc aattaaaacg actcattagc
      241 cgtattgcac ccagcccatc cagcgttatg gtggttggtg aaagcggcac gggtaaagaa
      301 gtcgtcgccc gtgcaatcca taagttgagc ggaagacgga ataaaccctt tattgctatc
      361 aactgtgccg cgattccgga gcagcttctg gagagcgaac tgttcggtta tgttaaaggt
      421 gcatttactg gcgcttctgc caacggtaaa acagggttga ttcaggcggc gaatacgggc
      481 acgctgtttc tcgatgaaat aggtgatatg ccattaatgt tgcaggctaa attactgcgc
      541 gctattgagg cacgtgaaat tctgccgatt ggtgccagta gcccaataca agtcgacatt
      601 cgcatcattt ctgcaactaa tcagaatttg gcccagttca ttgccgaagg taaattccgc
      661 gaagatctct tctaccgact taatgttatc ccgataactc tgccaccgct gcgtgaacgt
      721 caggaagata ttgaactatt ggtgcattac tttttacatc tgcatacccg tcgtctggga
      781 tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat tgcgtaagca tcgttggccc
      841 ggaaacctgc gcgagttaag caatttgatg gaatatctgg ttaacgtggt tccttcaggt
      901 gaagttatcg acagcacgct attgccgcca aatctgctga ataatggcac aacggagcaa
      961 agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg atgatgcggg cggcacggcg
     1021 ctggaggaga tggaaaagca aatgatccgc gaggcgcttt cacgtcataa cagcaagaag
     1081 caagttgctg atgaactggg catcggcatt gctacgctct atcgcaagat taagaaatat
     1141 gagttgttaa acacataacc tcagtgaatc cggcctgaat tcaggccgga tatcctttta
     1201 cttcgttttc tcgcaatcca gaactgtatt tacaatcatc tgataccccg tgcagcgaca
     1261 aagatttccc gccagtccgc gacgaatttc cgtaatggtt aatggcttct cgcgtggttt
     1321 cgccagcatt gccgtggtag ccataatcag gccaggcgta caaaacccgc actgcactgc
     1381 gccggatttc gcataagcct gctgaacatg agaaagtttt ccgcctttcg cttcaccttc
     1441 cagcgtgcgg atctcttttc cttcagccca ggcggcaagg tataagcaac tgtctattgc
     1501 tgtgccgtcg accaacaccg tacaggcacc acattcaccc acgcagcacc cttgtttgac
     1561 acttagcagt ccttgttcgc ggagtaattc cgagagcggc gtgcctggtg cggcgtgaag
     1621 ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt gtttcgctgt gattcattgc
     1681 aattttcccc ccgccgcggc gacggcttcg ctaatcactt ttttggtcat cgtctggatg
     1741 agatgcagac gaaactcttt actggcccgc catgaagaac gcggggcgac atcttgcagg
     1801 acagattcgc tgatagcttc cagcgtttgc aggtttaatg gcgcattttg tgcagtctgt
     1861 tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac caaatgccag gcgtaattcg
     1921 ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc caatcgttga aatatccatt
     1981 gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt gttctttcgg ctgtggcgga
     2041 aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca ctttgcccgg cccggtgtga
     2101 aagccattaa tcgggacgaa acgaacaccg cgcggggagt ggatctccag tttcgcgtca
     2161 taaattagcg ttggcgtggc agaatctgcg ctggtggcac cgttgcaaat atttccaccg
     2221 taggtagcga cgttacggat ctgcggtcca gcaatggacg tggccgcagc acataacgcc
     2281 gggagatgac gttgagttat aggatcttct attagctggg taaatgtcgt tgcagagccg
     2341 atacgtagcg agccatcttc cgccagcgta attccccgca gctccgccag attatgaata
     2401 tcaacaatat ggcgataacg gtcattgtga tggtggagct gaatcagtac gtcagtgcca
     2461 ccggcgagca gtttggcctg cgggttgtca gccagcaggt tgatggcatc ggcaagggtt
     2521 gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat cctcaaatca atcctgccag
     2581 atggaactct tcatataacc gttttggcgt cagcggcagt gtattgattg caacaccggt
     2641 agccatcttc accgcgttac gaatagcagc ggcaacagga attattggtg gctcacccag
     2701 tgacttatgt ccgtatgcgg attgcggctc attgatttcg acgaacgcgc tttccagttg
     2761 tggcagatcc ggcatggtcg gcattttgta atccagcaga ttggggttac ggaccacgcc
     2821 gcttttagca tcgatgatca tctcttcaaa tagcgcccag ccaatgccca ttcccattcc
     2881 gccgtgtacc tgaccttctg ccagcagtgg attaagaata tgccctgaat catgaacgtt
     2941 gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg accgtcagat caacaaaggt
     3001 acagccaaac gccggtgggt tagtggtggt tttgatggag ctttcagcag agagctgccc
     3061 gccgcgttca gggtggtaga aagcgtccat cgccaaatct tttaacgaca ttaacggctc
     3121 ttccggtcgt tcaaccagca cgatatggcc ttttatcagg gtcagattca tcgctgactg
     3181 atgtagcatg actgcggcgt gagcgatgat tttctctttt aataatagtg ccgcactgcg
     3241 cagcgcaggc gcggcaacat agctctggcg tgaggcaaat gcgccgggat cgaacggcgt
     3301 aacgtcggta tcttgagttg aaataacgcg aacgtcgctg accggaaccc ccacggtttc
     3361 tgccaccatt tgcgagaaga cggtgtcggc accctgaccg atttccgtcg cgccgctttg
     3421 cacgttgatg gttccatcct gattcatcag aaggcgcgcg cctgctattt ctacgccgac
     3481 aggccaggtg ttagaggtgt agctaaaaca ggcgacgcca acgccgcggc gcaaattgcc
     3541 ttgctggttc tggcattctg cacggcgttt ttcccattca aagattttcc ggcctttttc
     3601 aagacactcc ggcaaccctg cgctgtaaat acgtttgccc gtgagcggat tagcatctcc
     3661 ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca atacctaacg ctgtcgcggc
     3721 gtcatcaagc atagactcaa cggcaaatac gacttgtggc gcgccataac cacgcatcgc
     3781 accagccgag gggaggttgg tatagcaggt ctttgaactg taagcgtagg cacaacgagg
     3841 ataaaggtaa gcgactttat tcccccccgc agaagcgatg gagtgcccgt gagatgcata
     3901 agcgccggtg ttagacagaa catccagact ataacctttc aatgttccgt cgcggttcac
     3961 gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg gttgcgagga aacactcttc
     4021 acggctaagg gaaactttca ccggaatgcc gccaagcttg ctggtcagga atgccgccat
     4081 tggctcttcc agtacatcct gtttattacc aaaaccgcca ccgacaaatg gtttgatgac
     4141 tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc acgcggcgaa caatgtgcgg
     4201 gatctgggtg ctggaaacga tggtaatttg cgagtcatcc tccatccacg ccagcgatgt
     4261 tacgctttcc atgtgacaat gttgaataac gggggtctga tagtgcccct gtacctggta
     4321 gtcggcggca tcgattgttt gttggacatt acccgtcgac atcgtgcttt gtttcagtaa
     4381 attgccaccg ttatggattg gtgcagcgtc ttctgccagc gccgcttctg gcgtggtgat
     4441 aacgggtaat tcttgccact caatgctgac caattgcgcc gctttttctg ccgtgagttc
     4501 atcgcgggcc acgacgatgg caacggcgtc accatgatga cgaacatggc gagttagcag
     4561 tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc caggcatgcc cagctgtagc
     4621 gaatggaata tcaggcacat cttcccaggt aaaaatcgcc agtacgcccg gcaaacttct
     4681 ggcttgttca tcattaatac ttacggcata accatgtgcg atagggctac gtacatattt
     4741 cgcataacac atgcccgcca taacataatc gtcagtatat cgtgcccgcc cggtgacctt
     4801 agcaatggca tcgacgcgca tgcatgattc acccgtagcg gttgcttccc gcgcttccat
     4861 agaaatcccc tcgatttgaa catttggttc ttatggcaaa gatttagtct aagcaggcaa
     4921 gatttacgcc agaaacgacg ataagcgtta ggggaggtag gaagtgtgat gacgttaaaa
     4981 aaacacagat atgatacgaa acaggactct gaataggggc agtgatacgt atcaatatga
     5041 gtggcgataa aatcaatgtg ataaattggg attattacgc atcaggatgg gaactagggt
     5101 aaaaagtcag gaagttcgat acctctacag cgaatatttt gctgatgata cttccctttg
     5161 ccaaatgaaa ttaacctctt tctctattac cacgtttttc cagaagcaag agattgggta
     5221 tcaagaggct ggctgctatg ataaggcgtc ttgtttttta agcgaggaaa gattttgagt
     5281 gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt tgttatcggt tggactgctt
     5341 ttggcgggct gttcgggtag caaatcatcc gatacaggaa cgtattccgg ctccgtttac
     5401 accgtgaaac ggggggatac gctatatcgt atttcgcgca ccacgggaac cagcgtaaaa
     5461 gaactggcgc gactgaacgg catttccccc ccttacacca ttgaagttgg tcagaaacta
     5521 aaactgggtg gggcgaaaag tagcagtagt acacgtaaat caaccgccaa atcaacgacc
     5581 aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat catcctggcc gccagtaggg
     5641 caacgttgtt ggttatggcc aacgacaggg aaagttatca tgccgtattc gacagcagat
     5701 ggcggcaata aagggattga tatctcagct ccacggggta cacctattta cgccgcgggt
     5761 gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct acggtaatct catcatgatt
     5821 aaacacagtg aagattacat tacggcttac gcccataatg acacgatgct ggtaaataat
     5881 gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg ggagcacgga tgcggcatct
     5941 gttcgcctgc atttccagat tcgttaccgt gcaacggcaa ttgatccgct acgttacttg
     6001 ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta atcagcagtc agcagcgcgg
     6061 cacttgctaa ggagagcgta aggtttataa tgccttacgc atctcgaagc gggcgtagtt
     6121 caatggtaga acgagagctt cccaagctct atacgagggt tcgattccct tcgcccgctc
     6181 caatttatct tcgcctcatc aatctgccat ttttgctgta tctgattttc tatattatct
     6241 taattcaatc gcttcagact tttcgcaatc gccgtaatat ttccccattt tgttgtaaat
     6301 aaaaagcgtt tacgcaatgc aatccttttg taacaaccca ggtaaaacac cttattttca
     6361 agttgttaaa taagtggtta tctacgcttt taacaaacga ttgatggaat attttatgaa
     6421 agggaaatct gcattaactc ttctgctcgc tggtatattt tcgtgcggca catgccaggc
     6481 gacaggggca gaagtgacga gtgaatctgt atttaacatt cttaattcca cgggggcagc
     6541 aacagataaa agttatttat cgttaaatcc agataagtac cctaattatc gtcttctgat
     6601 tcactcagcc aaattaaaaa atgaaataaa gagccactat accaaagacg aaattcaagg
     6661 gttgttaaca ttaacagaaa acacacgaaa gttaacgttg acggagaaac cctggggaac
     6721 atttattctt gcttctactt tcgaagatga caaaacagcg gcagaaaccc actatgatgc
     6781 tgtctggctg agggatagtt tatggggcta tatggcatta gtatcagatc agggaaatag
     6841 tgtagccgca aaaaaggttc tgctcaccct gtgggattat atgtctacgc ccgaccagat
     6901 taagcggatg caggacgtca ttagcaaccc caaacgactg gatggagttc ccgggcaaat
     6961 gaatgctgtg catatccgat ttgacagcaa ctcccccgtg atggcagatg tgcaggaaga
     7021 aggcaagccg cagctgtgga accataaaca aaatgatgcg ttaggtcttt atcttgatct
     7081 ccttatacaa gcaatcgaca ctggtacaat taacgctgaa gattggcaaa aaggtgaccg
     7141 attaaaatcg gttgcgctgc tgattgctta tctggataaa gccaatttct atgttatgga
     7201 agattccgga gcatgggaag aagatgcccg attaaacaca tcttccgttg cactggtgac
     7261 ctctggactg gaacgtttgt cgaccttact ctcaaagaaa gactctgttt ttgtctctga
     7321 tttactacgc gaagctaaag ccaatgaact ggatgaacct ctttcaacaa cgaggttgaa
     7381 ccacctcata gataaaggtt atgaaagaat aacgttacag cttgatttag ggggagaatc
     7441 cccaggttat ctggaaaaag ataaacatta cagagaagcc gatgcggctt tacttaatgt
     7501 tatttatcct gcaaatcttt caaagataaa caccagaaga aaagagcaag ttctaaaaat
     7561 agttaaaaaa ctagcaggtc cgtatggtat aaaaagatac gagaaggata attatcagtc
     7621 ggcaaatttt tggttcaatg atattaaaac cgataccgat caaaattcgc atgcaaaaag
     7681 agaaaagagc ttcattccat caacagaagc agaatggttt tttgactcat ggtatgcaaa
     7741 atcagcagca attgtttata aagaatcaag aaaagaagag tatttgaatg actcagtgca
     7801 atttatgaat agatcattgg cccaaataac aggcgagaat atgattggtg caaatggcag
     7861 gagcgtacca gaaatggcac ttcccgaaag ctataactat atccacaagt cgggaacttt
     7921 gcatgaagcg ccaagcccta tcattccgct aaattggtcg aaagcttcaa tgactctcat
     7981 gttgaaagag atgtccaacc ttatcaatga tgagggaatt aaatagtttt cataatgagt
     8041 tacagcacaa cgttgggtat tgactgagca agatgaacga caaggacaac gacatcagcc
     8101 ttatgaggag caagaaaatg aaagcaaatt cgaaaacgat cagaaggatg aaagttaatg
     8161 ttcggtctga ggaaagtaaa tcgaaacact catacgtttg aaaccacttt tcaaaattgg
     8221 aaagaaaatg gcgaagatgt tgcattatta atgcccgaat ttagtgctaa atggctaccg
     8281 atagcagaag agagtggcag ctggtctgga tgggtattgc ttggagaaat atttccctta
     8341 atttcatctg agctcgctgg gatggcttta atgccagaaa cggaaaggtt aataggggaa
     8401 tggctcagtt tatccagttc cccgttaaat ctaaagcatc ctgaactaaa atataatcgt
     8461 ttgcgcgtag gtaaagtatt tgatggagta ttgagccctg ctcaaccact aataagaata
     8521 tggacagggg aattgaatct ttggttagat aaagtcacag tctgccaata cggaaacgct
     8581 ccagcgttag acaaaaaatc gttatattgc tccattcatt ttgtaattgg atttagtaaa
     8641 acatg
//
LOCUS       DAZFZC010000157         8529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFZC010000157 DAZFZC010000000
VERSION     DAZFZC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.159"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..408
                     /locus_tag="ACHKXP_004635"
     CDS             <1..408
                     /locus_tag="ACHKXP_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649483.1"
                     /translation="QALRSTDDMIKANVWHLYKEWIRSDDVSPIFIETEDNLRTFNTN
                     ELTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLT
                     QETLFSSKSHGKYALFPIFTASWRAHRIMNKGV"
     gene            631..1128
                     /gene="ubiC"
                     /locus_tag="ACHKXP_004636"
     CDS             631..1128
                     /gene="ubiC"
                     /locus_tag="ACHKXP_004636"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO7649484.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1141..2013
                     /gene="ubiA"
                     /locus_tag="ACHKXP_004637"
     CDS             1141..2013
                     /gene="ubiA"
                     /locus_tag="ACHKXP_004637"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO7649485.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2168..4591)
                     /gene="plsB"
                     /locus_tag="ACHKXP_004638"
     CDS             complement(2168..4591)
                     /gene="plsB"
                     /locus_tag="ACHKXP_004638"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO7649486.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4762..5130
                     /gene="dgkA"
                     /locus_tag="ACHKXP_004639"
     CDS             4762..5130
                     /gene="dgkA"
                     /locus_tag="ACHKXP_004639"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO7649487.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5240..5848
                     /gene="lexA"
                     /locus_tag="ACHKXP_004640"
     CDS             5240..5848
                     /gene="lexA"
                     /locus_tag="ACHKXP_004640"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO7649488.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5921..7246
                     /gene="dinF"
                     /locus_tag="ACHKXP_004641"
     CDS             5921..7246
                     /gene="dinF"
                     /locus_tag="ACHKXP_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO7649489.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARTLVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPVVGVWCYLLDGMFIGATRATEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7362..7571
                     /gene="yjbJ"
                     /locus_tag="ACHKXP_004642"
     CDS             7362..7571
                     /gene="yjbJ"
                     /locus_tag="ACHKXP_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO7649490.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7613..8128)
                     /gene="zur"
                     /locus_tag="ACHKXP_004643"
     CDS             complement(7613..8128)
                     /gene="zur"
                     /locus_tag="ACHKXP_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO7649491.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8446..>8529
                     /gene="yjbL"
                     /locus_tag="ACHKXP_004644"
     CDS             8446..>8529
                     /gene="yjbL"
                     /locus_tag="ACHKXP_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO7649492.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIK"
BASE COUNT         1978 a         2060 c         2299 g         2192 t
ORIGIN      
        1 caggccttac gatcaacgga tgatatgatc aaagcaaatg tttggcatct ttataaagag
       61 tggattcgta gtgatgatgt ttcacctata tttatagaaa ctgaagataa tttaagaacc
      121 tttaacacga atgaattaac acgaaacgat aatatcttta tcctgttctc ctcagtcgat
      181 gatgggccag ttatggtggt aagctcccag cgcttacatg atatgttgaa tcctacaaaa
      241 gataccaatt ggaattccac gtatatctac aaatccagac atgagatgtt gcctgttaat
      301 cttactcagg aaacactttt cagctccaaa tctcatggta aatatgcgct tttcccaatt
      361 tttactgcga gttggcgagc tcatcgtata atgaataagg gtgtttaagt aaaggaaaac
      421 atcaccgttc ctggcatcct ggacggtgat gcccctacgg ttgccctcgc cagcacgggc
      481 atcggtaaag cgtaaggttc aacatcgttt taccacttca tgcgattgtt gcgtttttgt
      541 tgcgtattag atcacttaat ttgctttaca tctcccgtaa acacttttct gcgatacaat
      601 gcctttacgt tatgtaacgg agagttcggc atgtcacacc ccgcgttaac gcaactgcgt
      661 gcgctgcgct attttaaaga gatccctgcc ctggatccgc aactgctcga ctggctgttg
      721 ctggaggatt ccatgacaaa acgttttgaa cagcagggaa aaacggtaag cgtgacgatg
      781 atccgcgaag ggtttgtcga gcagaatgaa atccccgaag aactgccgct gctgccgaaa
      841 gagtctcgtt actggttacg tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc
      901 ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg agctggcgtt acaaaaattg
      961 ggtaaaacgc cgttaggacg ctatctgttc acatcatcga cattaacccg ggactttatt
     1021 gagataggcc gtgatgccgg gctgtggggg cgacgttccc gcctgcgatt aagcggtaaa
     1081 ccgctgttgc taacagaact gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat
     1141 atggagtgga gtctgacgca gaataagctg ctggcgtttc atcgcttaat gcgtacggat
     1201 aagccaattg gcgcgttact gctgctctgg ccaacattat gggcgttgtg ggtggcgaca
     1261 ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg cgggtgtctg gctgatgcgc
     1321 gctgccggat gtgtggtgaa tgattatgct gaccgcaagt ttgatggtca tgttaagcgc
     1381 acggcgaacc gaccacttcc cagcggcgcg gtaacagaga aagaggcgcg cgcgctgttt
     1441 gtcgtgctgg tactgatttc gtttttactg gtgctgacgc tgaatacgat gaccattctg
     1501 ttgtcgattg ccgcgctagc gctggcgtgg gtgtacccgt ttatgaagcg gtatacccat
     1561 ctaccgcaag tggtgctggg cgcggcgttt ggctggtcga ttccaatggc ttttgccgct
     1621 gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc tcgccaatat tctctgggcg
     1681 gtggcttacg acacgcagta tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt
     1741 aaatccacgg caatcctgtt cggccaatac gataaattga ttattggtat tttgcagatt
     1801 ggcgtactgg cgctgatggc gatcatcggt gagttaaatg gcttaggctg gggatattac
     1861 tggtcaattc tggtggctgg cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc
     1921 gagcgtgaag cctgctttaa agcatttatg aataataact atgttggtct ggtactgttt
     1981 ttagggctgg caatgagtta ctggcatttc tgatgatgta aaaaagccgg atgatcatcc
     2041 ggctttcttc tgggttgcct gatgcgcggc gcttctcagg cctacacaac acatcgcaat
     2101 ttattgaatt tgcagattat ggaaggccgg ataaggcgtt ttcgccgcat ccggcaattc
     2161 tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa
     2221 tcaactccgc cagcaactga taaaccttca tcgtttctgc cggttcggca tcgccgctat
     2281 cgctgatata cccttcatca cgcagtgtca gcaccagaga actgaacacc gccttgtcga
     2341 agaactccgg cgcgttgatg ccgtgcagca cggagagacg ttgcgcgacg gtgcggctct
     2401 ctttctccag cgtaccgcgg ttgatcgacg ggttggcact caacaaccag aaggtgatgg
     2461 cataacgttg cagcgtttcg cgcgcgcctg cggccagcag ctgtagcgtg cgagaatgcg
     2521 ccgggttgat atgcaactca tcatcttgca gggtaatcag cccctgacgt tgcatctcat
     2581 ttgccagcgc atcaataacg tccggcaact cgtcgcgatc ccagcgcagg aacagctccg
     2641 ctttcagcat tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg gagatgtggc
     2701 gatgctgggt gacaattgcc gccatcagcg aaggcagcac caacatatgc gcaatgttgt
     2761 tgcgatagta ggtcatcaac accgcttgct cgcgcggcag aatgatgatg tcgccgatag
     2821 tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc
     2881 tggctgaagg aacggtagag tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc
     2941 agttgagttg ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgac gccagtagcg
     3001 cagtacagca caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat
     3061 cggcagcaat attattgacc gtcggcgtta accaggccgg acgcaccgct tcgatgggat
     3121 cgatagattc acgccagtct ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt
     3181 caccgaaatt gacgtaaccc tggccgagat tacgcagctt gcttaaaccg cgcagcatct
     3241 gcggcaggct ctctttctct ttcgtcgcac cgcgcagttc tttggcgtaa gtacccactt
     3301 ccatgacgtg ctcataaccg atatagatcg gaatcagcgt aatcggacgc gtgccgccac
     3361 gcagcatcgc ctgaatggtc atcgacagcg taccggtttt cggatccagc aaacgccccg
     3421 tacgggaacg accgccttcc acgaagtact cgacggaata accacggctg aacagttcgc
     3481 cgagatactc acggaaaacg gtggaataga gcttattgcc tttaaaagta cggcgaataa
     3541 agaacgcacc cagacggcgg aaaatcggcc cggccggcca gaagttcagg ttgatcccgg
     3601 cggcgatatg cggcggcacc agcccctggt ggtacagcac gtaagaaagc agcaggtagt
     3661 ccatgtgact gcggtggcaa ggcacatata ccagctcatg accgtcgtgg gccagctggc
     3721 gaacgcgctc ggcgttatgg acgttgatgc cctggtaaag gcggttccag gtaaagccca
     3781 gaatacggtc agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc tcttccatca
     3841 gcgcaatcgc gttctgctgc gctttttcat gggagatttt tttgctgcgc gcttcatctt
     3901 ctaccgcttt cgcaatagcg cgggaggcga gcagcttatt aaacagatcc tgacgagcag
     3961 gcagacgtgg gcctacggca gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg
     4021 ccagtttctg cgcgatagtt ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaaa
     4081 ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca cagtacagcg aaaaatttct
     4141 gtacgccgtt aagcatacgc agcggcgggt tcacttcgcc tttttcacgc cctggcgcgc
     4201 gaccaaacat caccgatact ggcaccatct gcacatccag atttgggttg ctacggtgca
     4261 aatcgagata gtcgtggaac agcttaatag actcttcttt cggcgtgtaa taggtgaata
     4321 cacgcggccc gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg atttccagcg
     4381 gctctaacgg gtcaggcagg tcatgtgcca gacactgggc gcgcaacgtc agcaaatctg
     4441 ctttcgagtt gtacggtaaa acgtacataa ttggacgaga ggtatccagc cccagttccg
     4501 gggcaggatc tgccggaata gacttgcttt ttaccaggat gcttaatggt aaattcagta
     4561 atttgtagta aattcgtggc cagccggaca taaacgatgt aaagcctctg gttaataatg
     4621 caaatgcgcg gcaaggatag cagaaagtca tgggaaattc tgtggtatcc gctcatgttt
     4681 cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg tacactgata ttgacgctca
     4741 taatgtaaaa aggttctttc aatggccaat aataccactg gattcacccg aattatcaaa
     4801 gctgctggct attcctggaa aggtttacgc gctgcatgga tcaacgaagc ggcattccgt
     4861 caggaaggcg tagcggtatt gttggcggtg gtcatcgcct gctggctgga tgtggacgca
     4921 attacccgcg tgctgcttat cagctccgtg atgctggtga tgattgtgga aatcctcaat
     4981 agcgccatcg aagcagtggt tgaccgaatt ggctctgaat accatgagct ttccggacgc
     5041 gctaaagata tgggatccgc cgcggtgctg attgccatta tcgtcgccgt gattacctgg
     5101 tgcattctgt tatggtcgca ttttggataa cccttccaga attcgataaa tctctggttt
     5161 attgtgcagt ttatggttcc aaaatcgcct tttgctgtat atactcacag cataactgta
     5221 tatacaccca gggggcggaa tgaaagcgtt aacggccagg caacaagagg tgtttgatct
     5281 catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg
     5341 tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg cacgcaaagg
     5401 cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga
     5461 agggttgccg ctggtaggtc gtgtggctgc cggtgaacca cttctggcgc aacagcatat
     5521 tgaaggtcat tatcaggtcg atccttcctt attcaagccg aatgctgatt tcctgctgcg
     5581 cgtcagcggg atgtcgatga aagatatcgg cattatggat ggtgacttgc tggcagtgca
     5641 taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg acgaagttac
     5701 cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa atagcgagtt
     5761 taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc attgaagggc tggcggttgg
     5821 ggttattcgc aacggcgact ggctgtaaca tatctctgag accgcgatgc cgcctggcgt
     5881 cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc atggcattcc tcacttcatc
     5941 tgataaagca ctctggcatc tcgccttacc catgattttc tccaatatca ccgttccgtt
     6001 gctgggactg gtcgatacgg cggtaattgg tcatcttgat agcccggttt atttgggcgg
     6061 cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg ctgttgctgt ttttacgcat
     6121 gagcaccacc gggctgactg cgcaggctta tggtgccaaa aatcctcagg cattagcccg
     6181 tacgctggtg caaccgttgc tgttggcgtt gggggctggg gcgttaattg cgctgctgcg
     6241 tacgccgatt atcgatctgg cgctgcatat tgttggcggt agtgaggcag tcctggaaca
     6301 ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg gcgtcgctgg cgaatctggt
     6361 attactcggt tggttactcg gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt
     6421 cggcaatatc ctcaacattg tgctggatgt ctggctggtg atggggctgc atatgaacgt
     6481 gcagggcgcg gcgctggcga cggttattgc ggaatatgca acattgctga ttggtctgct
     6541 aatggtgcgt aaaatcctca aactacgcgg aatttccggc gaaatgctga aaactgcctg
     6601 gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat atcatgctgc gttcgctgtt
     6661 gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg cgactgggga gtgacattat
     6721 cgctgttaac gcggttctga tgacgctact cacctttacc gcctatgcgc tggatggttt
     6781 tgcctacgcg gttgaagcgc actccggtca ggcatacggt gcgcgcgacg gtagccagtt
     6841 gctggatgtc tggcgggcag cgtgccgcca gtcggggatc gtagcgttac tgttttcggt
     6901 ggtttatttg ctggctgggg aacacatcat tgcgttactg acgtcgttaa cccagattca
     6961 gcagctggct gaccgctatc ttatctggca ggtgattttg ccggtggttg gcgtctggtg
     7021 ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc accgaaatgc gtaacagtat
     7081 ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc tgggtaatca
     7141 tgctttgtgg ctggcattaa ccgtctttct ggcgttgcgc gggctttctc tggcggctat
     7201 ctggcggcgt cactggcgca atggtacctg gtttgccgca acgtgacggt taaaaattct
     7261 gaataaataa tcctaagcca aattgctgac tacacttaat ctcacgttca gaagaaaagt
     7321 gaacgtactc tcattcacaa cctaacgatg aggtcttgat tatgaataaa gatgaagccg
     7381 gcggtaactg gaaacagttt aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg
     7441 atgatatgac gatcattgaa ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt
     7501 atggttatca gaaagatcag gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgaat
     7561 atcgctggta attaatccct cctgcccgac gtgtacaagg atgtacgccc tcttaacgcg
     7621 gtttcttttt cacctgcaca gagtgatcat gctggcactg ttcaggatga cgacacgctt
     7681 ccacttctac acatgccgca cagagcccat gtgcttcaat cacattatgc cgcagggcaa
     7741 accccatttt tgccgccagc gtatgcataa tgtcttccac gccttctgca cactcttctt
     7801 tcactgcgcc gcagcgatcg caaataaaca tggctgacgt atgggtgggc tgatcgaaca
     7861 gatgacagag cacataactg ttggtggatt ccaccttatg cacaaaacct tgctcaagca
     7921 gaaaatccag cgcgcgataa accgttggcg gcttggcttg cggttcagct tcgcgcagta
     7981 aatcaagcag atcataagcg ctgatagcgc catcctgcag actcatcagg cgcaacactt
     8041 ccaggcgctg tggggtcagg cgcacattac gctgcgcgca gattttttca gcctgcgcta
     8101 ataactcctg cgttgtggtc ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc
     8161 ttactttacc atgttccagg aaaaacaccg agaaccctta ttgttgccgt aatgttgatt
     8221 ttctgttttg taggtaaggt gttatgttgc cttgtcgtac cattatcaac acgataataa
     8281 ttaataattc atttttaaat aaattgcttc aagttaactc gctatttatt taactgggtt
     8341 taaacgcata cctgtagttc agaaattatg agcgattttt gatagtgacc agatggcaaa
     8401 agaattataa ggaggacacg gtacgacaaa aaaggtagat gaatcatgtt gaagataata
     8461 cccggtgcaa ctggatactt taataaaaca ctcaattcaa atcaattcga taatgaagat
     8521 gcaatcaaa
//
LOCUS       DAZFZC010000158         8495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFZC010000158 DAZFZC010000000
VERSION     DAZFZC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.160"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..1567
                     /locus_tag="ACHKXP_004645"
     CDS             5..1567
                     /locus_tag="ACHKXP_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO7649493.1"
                     /translation="MPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELATPLL
                     KSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPIFEKY
                     LMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYIN
                     DIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGLVMKA
                     YFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMS
                     ESKENIKHYSLMDFMNVDYSLLKWSNDHIINQSVAIIPALPKEQLLMLKGSVDEITPP
                     LSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNALPTL
                     FNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGFTAED
                     IMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYME
                     ALLKKASPHTVR"
     gene            complement(1536..3272)
                     /gene="aceK"
                     /locus_tag="ACHKXP_004646"
     CDS             complement(1536..3272)
                     /gene="aceK"
                     /locus_tag="ACHKXP_004646"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO7649494.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3553..4857)
                     /gene="aceA"
                     /locus_tag="ACHKXP_004647"
     CDS             complement(3553..4857)
                     /gene="aceA"
                     /locus_tag="ACHKXP_004647"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO7649495.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4887..6488)
                     /gene="aceB"
                     /locus_tag="ACHKXP_004648"
     CDS             complement(4887..6488)
                     /gene="aceB"
                     /locus_tag="ACHKXP_004648"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO7649496.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6757..7686)
                     /gene="metA"
                     /locus_tag="ACHKXP_004649"
     CDS             complement(6757..7686)
                     /gene="metA"
                     /locus_tag="ACHKXP_004649"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO7649497.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7843..8286
                     /gene="yjaB"
                     /locus_tag="ACHKXP_004650"
     CDS             7843..8286
                     /gene="yjaB"
                     /locus_tag="ACHKXP_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7649498.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRVLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            8299..>8495
                     /locus_tag="ACHKXP_004651"
     CDS             8299..>8495
                     /locus_tag="ACHKXP_004651"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649499.1"
                     /translation="MPAQAGEDAPASHPAFFSAPMQKGHPDGWPFAKFDAWQFMAGVL
                     PATLRAVASQRSNPLPADLSYS"
BASE COUNT         2176 a         2111 c         2092 g         2116 t
ORIGIN      
        1 tcgtatccct cgtagttctt tctctgcaaa tattaataat acagcccaga caaatgaaca
       61 ccaaaccctg agtgaattgt tttataaaga actcgaggat aaattttctg gcaaggagct
      121 ggcgacgcct ctattaaaaa gcttctcaga gaactgtaga caaaatggtc gtcatatctt
      181 tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg ataagaaaga
      241 aattacgata attaataaaa acgaaaacac gacacttact caaaccattg ccccaatatt
      301 tgaaaaatac ctaatggaaa ttttacctca acgctcagac actcttgata aacaagaatt
      361 aaacctaaaa tcagatagaa aagaaaaaga attcccaaga attaaactta atggtcaatg
      421 ttattttccg gggcgacccc aaaaccgtat agtatgccga cacattgctg cacaatatat
      481 taatgatatt tatcagaatg ttgattacaa accccatcaa gatgattact cttcagctga
      541 aaaatttctc acgcacttca acaaaaaatg caaaaaccag actttggcgt tggtttccag
      601 ccgtcctgag gggcgttgcg ttgctgcctg cggtgatttc gggctagtta tgaaagcata
      661 ttttgacaag atggaatcaa atggcatcag tgttatggca gccatattac tggtggataa
      721 ccatgctttg acggtccggc taagaataaa gaacacaact gaaggatgta cccattacgt
      781 ggtttcggtt tatgatccta atgtaactaa cgataaaata agaattatga gcgaaagcaa
      841 agagaatatt aaacactatt ctctgatgga ttttatgaat gtagattata gcctcctgaa
      901 atggtcaaat gatcatatta ttaaccaatc tgttgcaata attccagcac ttccgaaaga
      961 acagctattg atgttaaaag gatctgtgga tgaaataacc cctccattat caccagcaac
     1021 gatgaatttg ctaatggcaa ttggtcagaa tcaccaactt acgcaactga tgattcagct
     1081 ccagaaaatg ccagaactac atagaacaga aatgttgact gcctataata gtggacatat
     1141 gaacgttatt aatactattt ttaacgcatt acccactctg tttaatacgt ttaaattcga
     1201 taaaaaaaat atgaagcccc tcctcctggc aaataattct aatgaatatc ccggtttgtt
     1261 ttcagcgata cagcataaac aacaaaatgt tgtagagacg gtttatcttg ctttatctaa
     1321 ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc
     1381 cccacaaaaa tactctgcct ttgagttggc ttttgaattg ggtcaccggg ttattgctga
     1441 attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg
     1501 atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt
     1561 acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg
     1621 cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac
     1681 agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg
     1741 cccggcgaga cgctgtacca cggttcgctg gcaagttcgt cttccggata gcgcggcggc
     1801 gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc
     1861 acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta
     1921 gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga
     1981 ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcaat ataaagatgg
     2041 cgaatcacaa tttgttcgcc gagatcggtg attttttccg ctgcttcctg aagcagtaat
     2101 tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg
     2161 tccgccattc ggcccacgcg atcgtgctct ttcaccagtt gatagcaggc acgaacgtga
     2221 gcggcagaca tctctttctg cggcgcgaac ctgtctttga tgactttgaa tacccgatca
     2281 aagcccggca gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac
     2341 tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc
     2401 ttctggcagc cgatagccat atacaattca gcggtggttt tacctggcag aatttcccgt
     2461 agccactcga ccagcgctgc gggcagcggc gcataaacca taaaataaga acgcgcaaag
     2521 ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg
     2581 tcgtccgtct ggtggatcgg cagcaaaaat ggcaatgtgc cggaaggtgt gatcagtttg
     2641 cctaccagcc aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt
     2701 tccgcgaggt tgtctgtccc cagcgtttcc gtcagatggc gaataatgta atggatgtca
     2761 cggcttttat tctgccagcg caggcgcagc ggtaggtcgc tgataacgcg catcagtaga
     2821 gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag
     2881 cggcgctctg gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca
     2941 aataaccgac agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc
     3001 ggcaacagcc gggtgtaatg ctctttaaca cgtagtaaaa atgccgcgtc cgtactttgg
     3061 ccgttagtaa tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg
     3121 atacggtttt tcatcgcctg ctggacagca tgccagtcgg cctgttcgaa acgctgctgc
     3181 gcaccggagg tcacttcgag gaatcgacca tactgagcat cgaagccttg caaaatggtt
     3241 tgagcaatca ataattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt
     3301 gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata
     3361 agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct
     3421 tatccggcct acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag
     3481 gcatcgtgca ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac
     3541 ggttgttgtt gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga
     3601 cgtgccgccc tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg
     3661 gtgagatacg aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc
     3721 aacgtagtgc ttcataccct cgccctgggc ataggcgttt gccaggtcaa acatgttgaa
     3781 ccacatgctg tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag
     3841 ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg
     3901 cgagcagtta taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg
     3961 acgcgccagt tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc
     4021 atacgccagg ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc
     4081 actggtacgc tcgccggtaa taaattcgct gtcatacggg tcgcaatcgg aggtgatcag
     4141 atccgccgca tcagcatcgg tacgggcaac cagcagggtt ggaacgcccg tcacgtcagc
     4201 tgccagacgc gccgcgacca gtttctgaat agcttcctga gttggcacta aaactttgcc
     4261 gcccatgtga ccgcatttct tcactgacgc cagctgatct tcgaagtgaa ctgccgctgc
     4321 accggcttca atcatcgctt tcatcagttc aaaggcattc aggacaccgc caaaaccggc
     4381 ttccgcatcg gcaacgatcg gcaggaagta atcgacatag cgcggatcgc ccggctcaat
     4441 gcccgcggac cattggatct gatcggcacg acggaaggtg ttgttgatcc gctccaccac
     4501 agctggcacc gagtttgccg gatagagcga ctgatccgga tacatgctgg ccgccaggtt
     4561 agcgtccgcc gctacctgcc atcccgacag atagactgct tcaatacccg ctttcgcctg
     4621 ttgcagcgcc tgaccgccag tcagtgcgcc gaggctgttg atgtagcctt ttttcgactc
     4681 accgtgcagc agacgccaca ttttcgctgc gcccagttgc gccagcgtgc attcaggatt
     4741 gactgaaccg cgtaatttca ccacatcttc cgcactgtat gggcgagtaa tgccttccca
     4801 acgcggttga gtccactctt tctgtaattc ttcaatttgt tgtgtacggg ttttcatgtg
     4861 cagatgctcc atagttatgt ggtggtttac gctaacaggc ggtagcctgg cagggtcagg
     4921 aaatcaatta actcatcgga agtggtgatc tgttccatca agcgtgcggc atcgtcaaaa
     4981 cgcccctggg agaaacgttc ttcgcccagt tcgctggcaa tgactttcat ctcttcgccc
     5041 agcatctggc ggaacaaggc tttggtcacc ggtttgccat tgctcaacgt tttttgatga
     5101 tggatccact gccagatcga ggtacgggaa atttcagccg tcgccgcatc ttccatcagg
     5161 ccataaatcg gcacacagcc gttgccagag atccacgctt cgatgtactg cacagccacg
     5221 cgaatgttgg cgcgcatacc ttcttcggtg cgttcaccat cacaaggtgc cagcagctga
     5281 tcggcagtaa tcggcgcgtc ttgttcgcgc atcacttcaa gctgattttt acgggagccg
     5341 agaatgtcgt tgaataccgc cattgccgtg tccgcaaggc ctgggtgagc gatccatgtg
     5401 ccatcgtgac cgttattggc ttccagcgat ttatccgctt ttactttgtt gagcacctgg
     5461 ttattgtgct cttcatcttt gctcggaata aacgccgcca tgccgcccat cgcaaaagca
     5521 ccgcgtttat ggcaggtttt aatcaacagg cgtgagtaag cattcaggaa tggtttatcc
     5581 atcgtcactg cctgtctgtc tggcaggacg cgatcgggat agtttttcaa cgttttgata
     5641 tagctgaaga tgtaatccca acgaccgcag ttcagaccaa caatatggtc acgcagcgcg
     5701 tgaaggattt catccatctg gaacacggcg ggcagcgttt caatcagcaa cgtcgccttg
     5761 atggtgccgc gcggcagatt aaagcgatct tctgcatagc tgaagacttc gctccaccag
     5821 gccgcttcct gccaggactg ggttttcggc agatagaaat agggaccact gccctttgcc
     5881 aacagtgcct gatagttgtg gaagaaatag agcgcaaaat caaacaggct gccggggatt
     5941 gcctcaccac gccaggtgac atgtttttcc ggcaagtgca gaccgcgtac ccgacaaatc
     6001 aaaaccgctg gattgggctt gagctgataa attttgcctg cttcattggt atagctgatg
     6061 gtgccgttaa ctgcatcacg caggttaatt tgcccgtcga tcactttgtt ccagtccggg
     6121 gccagtgaat cttcgaaatc ggccataaag actttcacat tggcgttcag tgcgttgatc
     6181 accatcttgc gctcaaccgg gccagttatc tcgactcgac gatcttgtaa gtccgcagga
     6241 ataccacgaa ttttccagtc tgtatcacga atggaagctg tttccgaaat aaaatcaggc
     6301 aacgttccgt tatcaatatc ttgctgctgc tgaatgcgcg ctgccagaag tttattgcgt
     6361 tgtggcgtaa aatgcgtcac cagctcagtc agaaattcta ccgcttcggc agtaagaatt
     6421 tgcttctcct gctcgccata cggccttgtg aaagccagtt catcggttgt tgttgcctgt
     6481 tcagtcatcg tgcagctcct cgtcatggat ccgaaaactt ccccactgtg aacgaaggat
     6541 cgttgtgcgc ttttcgttca gcacaactaa gactactcat ttaaaaatca aaatcaaaaa
     6601 caatttccat tttaattaaa aatacttgat aacttattga taacaatatg aataaaattt
     6661 attcacttgc tgaggtgcgt ttcggaaata aaaaaggcac ccgaaggtgc ctgaggtaag
     6721 gtgctgaatc gcttaacgat cgactatcac agaagattaa tccagcgttg gattcatgtg
     6781 ccgtagatcg tatggcgtga tctggtagac gtaatagttg agccagttgg taaacagcaa
     6841 attaccgtga ctacgccagc tcgctcgcgg tttgttttgc ggatcgttgt gcgggaaata
     6901 gttatacggt acatccgggt ctagtccggc ttccacatcg cggaaatatt cctgcgccag
     6961 cgtttgcgca tcatattcgg gatggcccgt cacaaaggca atgcgcttat ctttactggc
     7021 aaacagatat gcatcccctt cttccgtctc tgccagaatt tccagatcgg tgtaatcacg
     7081 aatcaacgct gccggaaagt cagcatagcg cgaatgcggt gccaggaatg aatcatcaaa
     7141 gccacgcgtc agaagcgcat gaggatggag aatatgatgc tcgtaaacgc cagagagttt
     7201 gtcggtgcga gtttgcttag gaatgccgta gaggatattg agcgcggcct gtaccgccca
     7261 gcagacaaac agcgtcgagg tgacgtgatc tttcgaccac tccagcacct gtttgatctg
     7321 cggccagtaa gcgacatcat taaactccac caggcccagc ggcgcaccgg ttacaatcag
     7381 accgtcaaag ttctgttcct gaatatcttc aaagttacag tagaagttgt tcagatgctc
     7441 tgcgggcgtg ttgcgcgatt cacgggaatc gatgcgcaac agctgaatat cgacctgcaa
     7501 aggtgagttt gaaagcaggc gcagaaactg attttcagtt tcaatcttct tcggcatcag
     7561 gttaaggatc agaaccttaa gtggacgaat ttcctgacca gacgcacgag aagttgtcat
     7621 cacaaagacg ttttcttcac gcaagaaatt gacggcgggt agctcgtccg gcacacgaat
     7681 cggcataacc tgattacctc actacatacg cttatacgtt tagacatcca gatagctgaa
     7741 gataaccaga aaatttgcca atgtcgagcc tgcatgttga aggtgagaaa gtttcaagga
     7801 aaagactacg ttagaatata agaatgacta aaaggagaga aaatggttat tagtattcgc
     7861 cgctcgcggc atgaggaagg ggaagaactc gttgcgattt ggtgtcgttc ggtcgatgcc
     7921 acccacgatt ttttatcagc ggagtatcgg gccgagctgg aagaactggt gcgttccttt
     7981 ctgccggaag caccgttgtg ggtcgcggtt aatgagcggg atcagccggt tggatttatg
     8041 ttgttaagtg ggcagcatat ggatgcgctg tttatcgatc ctgatgtgcg cggctgcggc
     8101 gtaggtcggg tgctggtgga gcatgcgctc tcgatggcac cggaactgac aaccaacgtt
     8161 aatgagcaaa atgagcaggc ggttgggttc tataagaagg tggggtttaa ggttacggga
     8221 cgctctgagg tggacgattt ggggaaaccg tatccgttgc tgaatctggc gtatgtggag
     8281 gaataagctg cccctgccgt gcctgcccag gctggagaag acgcgccagc gtcgcacccg
     8341 gcattttttt ctgcgccaat gcaaaaaggc catcctgacg gatggccttt cgctaaattt
     8401 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa
     8461 cgttcaaatc cgctcccggc ggatttgtcc tactc
//
LOCUS       DAZFZC010000159         8114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFZC010000159 DAZFZC010000000
VERSION     DAZFZC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.161"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            569..1852
                     /gene="ydfJ"
                     /locus_tag="ACHKXP_004652"
                     /pseudo
     CDS             569..1852
                     /gene="ydfJ"
                     /locus_tag="ACHKXP_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1941..3401
                     /locus_tag="ACHKXP_004653"
     CDS             1941..3401
                     /locus_tag="ACHKXP_004653"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO7649500.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADVWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFQAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIQAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3437..3640)
                     /gene="ydfZ"
                     /locus_tag="ACHKXP_004654"
     CDS             complement(3437..3640)
                     /gene="ydfZ"
                     /locus_tag="ACHKXP_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO7649501.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3817..4503)
                     /gene="rspR"
                     /locus_tag="ACHKXP_004655"
                     /gene_synonym="ydfH"
     CDS             complement(3817..4503)
                     /gene="rspR"
                     /locus_tag="ACHKXP_004655"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO7649502.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4592..5338)
                     /gene="ydfG"
                     /locus_tag="ACHKXP_004656"
     CDS             complement(4592..5338)
                     /gene="ydfG"
                     /locus_tag="ACHKXP_004656"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO7649503.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5475..7520
                     /gene="dcp"
                     /locus_tag="ACHKXP_004657"
     CDS             5475..7520
                     /gene="dcp"
                     /locus_tag="ACHKXP_004657"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO7649504.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFTAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGINFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7565..8083)
                     /locus_tag="ACHKXP_004658"
     CDS             complement(7565..8083)
                     /locus_tag="ACHKXP_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO7649505.1"
                     /translation="MNINRDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2050 a         1858 c         2062 g         2144 t
ORIGIN      
        1 tagttgacaa tttttatctt gggtgttctt aaagttcgta gataaacatt gtcgtttcag
       61 gtatacagga atgctaacag gtggcggcaa aaatcaggcg gtttatggcg caatctgaag
      121 tggcaactgc aaactatctt atgcagagac tctacacgga ttgggtttaa aagtacacat
      181 agataacaat ttttatctga agaagaaaaa tatcaaggtg atatagccta tatgcctttg
      241 atgcggagga atgaatgtga tgggagtgat gtatctgaat agttgaaaaa ccgcagacac
      301 gccttatgca agaacgtgct gcggttggct ggtaaatttt tcgatagtgc gagtattgaa
      361 tgatttccag ccgttattga ttttacgcgt aaatccatga aaaaactact tatctgttgg
      421 ggagtttttt ggggcatata tgggacagaa ataggcccca gatagacact gagatcgaac
      481 ttaggatgct tttaaaaaaa tgcaactatc tgaaaaaacc tagaaaacgc caaggaaacc
      541 acaggatggg aaaaaacacc tgtgaattat ggatttccag ttatattcgc tcggcgcagc
      601 gttagtgttt catgaaatat tttttcctga atcatcaacg gcaatggcgt taattttggc
      661 aatgggaacc tacggtgcag gttatgtggc gcgtattgtc ggagcattta ttttcggcaa
      721 aatgggcgac agaatagggc gtaaaaaagt gctctttatt accatcacca tgatggggat
      781 ctgtaccacc ttaattggtg tgttaccgac ctatgcacag attggtgttt ttgcccccat
      841 cttgctggtg acgctgcgta ttattcaggg attgggtgca ggtgcggaaa tttccggtgc
      901 cggtacgatg ctggcggaat atgcgccaaa aggtaagcgc ggaattatct cctcatttgt
      961 agctatggga actaactgcg gaaccttgag cgcaacggca atctgggcct ttatgttctt
     1021 cattctcagt aaagaggaac tgctggcgtg gggatggcgt ataccgttcc tggcgagcgt
     1081 tgtcgtgatg gtctttgcta tctggttacg tatgaatctg aaagaaagcc cggtctttga
     1141 gaaggttaat gacagtaacc aaccgacagc aaaacctgca cctgctggta gcatgttcca
     1201 gagcaaatcc ttctggctgg caacagggct gcgttttggt caggcgggta actccggttt
     1261 aattcagact ttccttgcag gctatttagt gcagacgtta ttgtttaaca aagcaattcc
     1321 aacagatgca ttgatgatta gttcgattct cggctttatg accattccgt tccttggttg
     1381 gttatccgat aaaattggtc gccggatccc gtatattatt atgaatacct ccgcgattgt
     1441 gctggcatgg ccaatgcttt ctatcatcgt agataaaagc tatgccccga gcaccattat
     1501 ggttgcactg attgtgattc ataactgtgc ggtgctggga ttatttgctc tggaaaacat
     1561 taccatggca gaaatgttcg gctgtaaaaa ccgctttacc cggatggcta tttctaaaga
     1621 aattggtggt cttattgctt ccggttttgg tcctatcctg gcgggtattt tctgcaccat
     1681 gacggaatcc tggtatccga tcgccattat gatcatggca tattcagtga ttggtttaat
     1741 ctctgcgctg aaaatgccag aggtgaaaga ccgtgattta agtgcgctgg aagacgctgc
     1801 ggaagatcaa ccgcgtgttg taagagctgc gcaaccttcc agaagtctgt aaatcctcaa
     1861 tcccttctct taccggagag gggattttta ttcatataac aaaacatgta gcttgccata
     1921 tttaaattta aggaattgtt atgggaaata atttgttatc agcaaaagcg acactccctg
     1981 tttatgatcg taataacctg gccccaagaa ttgttcattt aggctttggt gcatttcacc
     2041 gtgcgcatca gggcgtgtat gccgatattc ttgctacaga acatttcagt gactggggat
     2101 attatgaggt caacttaatc ggcggcgaac agcaaattgc cgatttacaa cagcaagata
     2161 atctttatac cgttgcggaa atgtcggccg atgtgtggac ggctcgcgtc gttggcgtcg
     2221 ttaaaaaagc cttgcacgta cagatggatg gcttagaaac cgtgttggct gcgatgtgtg
     2281 aaccacaaat tgcgattgtc tctctgacaa tcaccgaaaa agggtatttc cactctccgg
     2341 cgaccggaca gttaatgctc gatcacccga tggtcgctgc cgacgtgcaa aatccccacc
     2401 agccgaaaac agcaacaggg gtgattgttg aggcgctggc tcgccgtaaa gcggcaggac
     2461 ttcccgcatt taccgtcatg tcatgtgaca acatgccaga aaacggtcat gttatgcgtg
     2521 acgttgtcac ttcctacgcg caagccgttg atgtaaaact ggcacaatgg atcgaagata
     2581 acgtgacttt cccatcaaca atggtggacc gtattgtgcc cgcagtgaca gaggatacgc
     2641 tggcgaaaat cgaacaactt accggtgtgc gcgatcctgc gggcgttgcc tgtgaacctt
     2701 tccgccagtg ggtaatagaa gataactttg ttgccggacg tccggaatgg gaaaaagcgg
     2761 gagccgaact ggttagtgat gtgctgcctt atgaagagat gaagttgcgc atgctcaacg
     2821 gcagtcattc attccaggcg tatctggggt atcttgccgg atatcagcac attaatgact
     2881 gcatggaaga tgaacattat cgtcatgcgg cgtatggctt gatgttgcag gaacaagcgc
     2941 cgacgctgaa agtgcagggc gttgatttgc aagattacgc taaccgatta attgaacgct
     3001 atagcaaccc ggcgttacgt catcgaacct ggcagattgc gatggatggc agccagaaat
     3061 tgccacagcg gatgttggat tctgttcgct ggcatctggc gcatgacagc aagttcgatc
     3121 tgctggcact gggcgtcgcg ggttggatgc gttatgtcgg tggtgttgat gaacagggaa
     3181 atccgattga aatcagtgac ccactgttac ctgttattca gaaggctgta caaagtagtg
     3241 ccgaagggaa agcgcgcgtc cagtcattgc tggcgattca ggcgattttt ggtgatgatt
     3301 tgccagacaa tagcttgttt actgcaaaag tgacggaagc gtacttgtct ttattagcgc
     3361 atggcgcgaa agcgaccgtg gcaaaatatt ccgtgaagta aaaaaagcaa tgccgccatc
     3421 ggcggcattc acacgcttag ttcatgccga gacgaatcag gtccagtggt gccagtttct
     3481 cttcacaacc ttcaacaact acggtttttc cgcggcggat ttgctcagcg gtcagacctt
     3541 cagtatcaat cgacaggatc ttgccagtgt gaccggtgcc gctaaccata acacggctgc
     3601 cagtggtgat tgcgttacgg ttacgatcgt aggtcgtcat aggtgttttc tcctttctga
     3661 tttacagtcg aagcccacgt aacgggcgca atataaatac tcctgtcgag tgaaatattt
     3721 tttgtttttg atcaagctca catctttttt gatgcatgca tgagccataa ggcgggggaa
     3781 aagactctca ccccggatgg gatgagagga aatgaattac tcttcgctaa accagtcgct
     3841 gttttcctgg cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc
     3901 acgttctacc gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg
     3961 taacagcatt tctggtggag aaacgtggtc gaaactcata tagcgcacgc gatcaacggt
     4021 tgctttcagg ttctcaatgg tatcccacgc cagttgacag tcggcaatct gcgtcaggag
     4081 ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat
     4141 gcgttgttgg tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc
     4201 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     4261 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     4321 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     4381 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     4441 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     4501 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     4561 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     4621 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     4681 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     4741 ttttttccgc tttaccatca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     4801 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     4861 tcagactaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     4921 gccagctacc tgccgttgag ccgatgttaa taatatgacc atgattacgt tcaaccatac
     4981 ccggtaagac ggctcgcgtc atatatacca ggcctttgtt gttggtatca atcatcgttt
     5041 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     5101 ttaccaggat atcaatattg caccactcgg caggaagcga tgccagcatc tcttcaatag
     5161 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     5221 actcctgcaa ccgctcctgg cgacggccag tggcgataac tttatgccct tgttgaataa
     5281 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     5341 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     5401 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     5461 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     5521 tggctcccca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     5581 tgcagcaaaa acgggcagaa attgccgcta ttgcgcttaa cccgcaaaca cctgatttca
     5641 acaatactat tctggcgctg gaacaaagcg gagaattact tacccgcgtt accagcgtct
     5701 tttttgcgat gactgcggcg catacgaatg atgaattaca gcgtcttgac gagcagtttt
     5761 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     5821 tagatgctgt ctggcaacgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     5881 tggaggtgat tcatcaacgt tttgtccttg ccggagccaa acttgcgcaa gctgataaag
     5941 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcgat
     6001 tactggcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     6061 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     6121 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgag atgcgcgatc
     6181 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     6241 atgatacccg cgctatcatt caacgtctgg tggagatccg tgcacaacag gctacattac
     6301 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acacctgaag
     6361 cagcgctcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgca agcgatgaat
     6421 tagcctccat acaggcggtt atcgataagc agcagggcgg gtttaccgcg cagccgtggg
     6481 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     6541 tcaaaccata ttttgaatta aacacggtgt tgaatgaagg agtattctgg accgcgaatc
     6601 agctcttcgg tattaatttt gtcgaacgtt ttgatattcc tgtctaccat cctgacgttc
     6661 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     6721 tcgcccgtga ttcaaaaagc ggaggtgcat ggatgggcaa ttttgttgag caatcaacgc
     6781 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaacct gctgccggtg
     6841 agcctgcgtt gttactctgg gatgatgtca taaccttatt ccatgaattt ggtcatacac
     6901 tgcacggcct ttttgcccgc cagcgttatg ctacgctttc cggcaccaac acgccgcgtg
     6961 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     7021 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     7081 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     7141 ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt
     7201 tcgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     7261 gcagcagtta tttcgcccat atttttggtg gcggatatgc cgcaggttat tacgcttatc
     7321 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     7381 cgcgtgaaaa tgggctgcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     7441 tggaacgcct gtatcgacaa tggcgcggta aggctcctca gattatgccg atgcttcaac
     7501 atcgtggctt gaacatataa acaccaccaa tggctgtcct ggagatatcg ccaggccagc
     7561 ctggttatag cagtaattgc agcagctggg cgagggcaaa cctcaccgct aaagctaata
     7621 ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta gttctggcct ttaatatgcc
     7681 acgcgaacca gacggtaccc gctggcgtac catcttcacc gccctccggt ccgccgtagc
     7741 cggtaatggc aatactgaca tcagcatccg cacgctctat ggcaccggtt gccatttctg
     7801 ctgccacttt ctcactcacc gcagaatatc gttcaagaga ttgctggctt acgctgagga
     7861 ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat
     7921 cttcagctgc acacagggcg ctagccaact taccgccggt gcatgattcg gcggtagtta
     7981 agcgtaattg atccgcgata agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt
     8041 cggtatctgc aagttgtaca attttatctc tattaatgtt cactgttttt tgtccacgct
     8101 tttatatgta aagg
//
LOCUS       DAZFZC010000160         7871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFZC010000160 DAZFZC010000000
VERSION     DAZFZC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.162"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            139..228
                     /gene="kdpF"
                     /locus_tag="ACHKXP_004659"
     CDS             139..228
                     /gene="kdpF"
                     /locus_tag="ACHKXP_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO7649506.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            228..1901
                     /gene="kdpA"
                     /locus_tag="ACHKXP_004660"
     CDS             228..1901
                     /gene="kdpA"
                     /locus_tag="ACHKXP_004660"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO7649507.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGTDSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1924..3972
                     /gene="kdpB"
                     /locus_tag="ACHKXP_004661"
     CDS             1924..3972
                     /gene="kdpB"
                     /locus_tag="ACHKXP_004661"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO7649508.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWITVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMCLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3981..4553
                     /gene="kdpC"
                     /locus_tag="ACHKXP_004662"
     CDS             3981..4553
                     /gene="kdpC"
                     /locus_tag="ACHKXP_004662"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO7649509.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4546..7230
                     /gene="kdpD"
                     /locus_tag="ACHKXP_004663"
     CDS             4546..7230
                     /gene="kdpD"
                     /locus_tag="ACHKXP_004663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO7649510.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDNRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7227..>7871
                     /gene="kdpE"
                     /locus_tag="ACHKXP_004664"
     CDS             7227..>7871
                     /gene="kdpE"
                     /locus_tag="ACHKXP_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO7649511.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRQSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFIT"
BASE COUNT         1612 a         2072 c         2340 g         1847 t
ORIGIN      
        1 ttaatactaa acattagtta aatcatggct tttgccattt ttatactttt tttacacccc
       61 gcccgcagat ttttgcgaaa tctttgcagc cagaattcta cccttccggt atcactttta
      121 ggccactgga ggtgcactgt gagtgcaggc gtgataaccg gcgtattgct ggtgttttta
      181 ttactgggtt atctggttta tgccctgatc aatgcggagg cgttctgatg gctgcgcaag
      241 ggttcttact gatcgccacg tttttactgg tgttaatggt gctggcgcgt cctttaggca
      301 gcgggctggc gcggctgatt aatgacattc ctcttcccgg tacaacgggc gttgagcgcg
      361 tactttttcg cgcacttggc gtctctgacc gtgagatgaa ctggaagcaa tatctttgtg
      421 ccattctcgg cctgaacatg ctggggctgg cggtgctgtt ttttatgttg ctcggtcagc
      481 actatctgcc gcttaatcca cagcagttgc cagggctgtc gtgggatctg gcgctgaata
      541 ccgccgtcag ctttgtcacc aataccaact ggcaatctta tagcggtgaa accacgttga
      601 gctatttcag ccagatggcg ggcttaacgg tgcaaaactt tctttctgcc gccagcggga
      661 ttgcggtgat ttttgccctc atccgtgcgt ttacccgcca gagcatgagc acgctcggga
      721 atgcctgggt cgatctgcta cgcatcacgt tatgggtgct agtccctgtg gcgttgttga
      781 ttgcactgtt ttttattcaa caaggtgcgc tgcaaaactt tctgccttat caggctgtga
      841 ataccgttga aggagcgcaa cagctgttac ccatggggcc tgtagcttct caggaagcga
      901 tcaagatgct cggtactaac ggcggtggct tctttaatgc caactcgtcg catccgtttg
      961 aaaacccaac cgcactgacc aacttcgtgc agatgctggc gatcttcttg atcccaacgg
     1021 cgctgtgctt tgcctttggt gaagtgacgg gcgatcgccg ccaggggcgc atgttgctgt
     1081 gggcgatgtc agtgattttt gtcatctgcg taggcgtggt gatgtgggca gaagttcagg
     1141 gtaatcctca tctgctggca ctgggcacgg acagcagcat caatatggaa ggtaaagaga
     1201 gccgtttcgg cgtgctggtc agtagcctgt ttgcggtcgt gacgacggcg gcttcctgtg
     1261 gcgcggtgat tgcgatgcat gattcgttta ccgctctcgg tggcatggtg ccgatgtggc
     1321 tgatgcaaat tggtgaagtg gtgttcggcg gtgtcggttc tggtctttac ggcatgatgc
     1381 tgtttgtcct gctggcggtg tttattgccg ggctgatgat tggtcgtaca ccggaatatc
     1441 tgggtaaaaa aatcgacgta cgcgagatga aactgactgc actggcaatt ctggttaccc
     1501 cgacgctggt gctgatgggc gcggcgttgg cgatgatgac cgacgccgga cgtagcgcca
     1561 tgctcaaccc tggcccgcat ggttttagcg aagtgctgta cgccgtgtca tccgccgcta
     1621 acaacaacgg cagcgccttt gccggattaa gcgccaactc tccgttctgg aactgtttac
     1681 tggcgttctg catgtttgtc ggtcgcttcg gggtgattat cccggtgatg gcaattgccg
     1741 gttcgctggt gagtaaaaag agccaacccg ccagctccgg cacgctgcca acgcacggcc
     1801 cgctgtttgt tggcctgtta atcggcaccg tgttgctggt tggcgcactg acctttatcc
     1861 ctgccctggc gcttggtccg gtggcggaat atctctcctg atgatattga gtgagcactg
     1921 aatatgagtc gtaaacaact ggcgctattc gaaccaacac ttgtcgttca ggcgctgaaa
     1981 gaagcggtga aaaaattaaa cccgcaggcg caatggcgca atccggtgat gtttatcgtc
     2041 tggatcggca gtctgctgac cacctgtatt agcatcgcga tggcaagcgg tgcgatgccc
     2101 ggcaatgcgc tgtttagcgc ggccattagc ggttggctgt ggatcaccgt actgttcgct
     2161 aatttcgccg aggcgctggc agaaggccgc agtaaagcgc aggccaacag tctgaaaggg
     2221 gtgaaaaaaa ctgcctttgc ccgcaagctg cgtgagccga aatatggcgc tgcggcggac
     2281 aaagttcctg ccgaccaact tcgtaaaggc gatatcgtac tggtagaagc tggcgatatt
     2341 atcccctgcg atggtgaagt tattgaaggg ggtgcatcgg tcgatgaaag cgccatcacc
     2401 ggggaatcgg caccggtgat ccgtgaatcc ggcggcgatt ttgcctccgt caccggcggc
     2461 acgcgtattc tttctgactg gctggtgatt gagtgtagcg ttaaccccgg cgagacattt
     2521 ctggatcgga tgatcgcgat ggtggaaggc gcacagcgac gcaaaacgcc gaacgaaatt
     2581 gccctgacca ttctgctgat tgccctgact atcgtctttt tactggcaac cgccacgctg
     2641 tggccgtttt ccgcgtgggg cggtaatgca gtcagcgtaa cggtactggt ggcgctgctg
     2701 gtctgtctga tcccaaccac tattggcggc ctgttgtcag cgatcggcgt cgccgggatg
     2761 agccggatgc taggcgcgaa tgtgattgcc accagcggac gtgcagttga agcggcaggt
     2821 gacgttgacg ttctgctact ggataaaacc ggcaccatca cactcggtaa ccgtcaggcg
     2881 tcggagttta tccccgcgca gggcgtggat gaaaaaacgc tggctgacgc cgcacaactg
     2941 gcttcgctgg ctgatgaaac gccggaaggc cgcagtattg tgatcctcgc caagcagcgt
     3001 tttaacctgc gcgagcgcga tgtgcagtcg ctccatgcca cctttgtacc gtttactgcg
     3061 caaagccgga tgagcgggat caacatcgac aaccgcatga tccgtaaagg ttctgtcgat
     3121 gccattcgtc gccatgttga ggctaacggt ggtcacttcc ctaccgatgt tgatcaaaaa
     3181 gtcgatcagg ttgcgcgtca gggagccacg ccgctggtgg tggtggaagg ttctcgtgtg
     3241 ctgggcgtta ttgcgctgaa agatatcgtc aaaggcggta ttaaagagcg cttcgcccag
     3301 ctgcgcaaaa tgggcattaa aacggtgatg attaccggcg ataaccgtct gactgccgcc
     3361 gcgattgctg cggaagcggg tgtcgatgat tttctcgccg aagcgacacc ggaggccaag
     3421 ctggcattga tccgtcagta tcaggcggaa ggtcgtttgg tagcgatgac cggcgacggc
     3481 accaacgatg ctccggcgct ggcgcaggca gatgtcgcgg tggcgatgaa ctccggcacc
     3541 caggcggcga aagaggcggg caatatggtc gatctcgact ctaacccgac caagttgatc
     3601 gaggtggtgc acattggcaa acagatgctg atgacccgtg gctcgctgac caccttcagc
     3661 attgccaacg atgtggcgaa atacttcgcc attattccgg cggcattcgc ggcaacgtat
     3721 ccgcagttaa atgcgctgaa catcatgtgc ctgcattcgc ccgactccgc aatcctcagt
     3781 gcggtgattt tcaacgcctt gattatcgtc tttttgattc ccctggcgtt aaaaggcgtg
     3841 agttataaac cgcttaccgc ttctgccatg ttgcgccgta acttatggat ttacggtctg
     3901 ggtgggctgc tggtgccgtt tatcggtatc aaagtcattg atttactgct gaccgtttgc
     3961 ggtctggtgt gaggtttacc atgagtggat tacgtccggc attatcaaca tttatctttc
     4021 tgttattgat tactggcggc gtttacccgc tgctgaccac cgtactgggg caatggtggt
     4081 ttccctggca ggccaatggt tcgttgattc gtgaaggtga tacggtgcgc ggttcggcat
     4141 taatcgggca gaattttacc ggcaacggct attttcatgg tcgcccgtcg gcaacggcag
     4201 aaatgcccta taatccacag gcttctggcg ggagcaatct ggcggtcagt aaccctgagc
     4261 tggataaact aatagccgca cgcgttgctg cattacgggc cgctaacccg gatgccagcg
     4321 cgagcgttcc ggttgaactg gtgacggcat cggcaagcgg gctggacaat aatatcaccc
     4381 cgcaagcggc ggcctggcaa atcccacgcg tggcgaaagc gcgtaatctc agcgttgaac
     4441 agctcacgca actgatcgca aaatacagcc aacaaccgct ggtgaaatat atcggccagc
     4501 cggttgtcaa cattgttgaa ctcaatctgg cgctggataa acttgatgaa taacgaaccc
     4561 ttacgtcccg accccgatcg tctgctggaa caaactgccg cgccgcatcg ggggaagctg
     4621 aaagttttct tcggtgcctg tgcaggcgtc gggaagacct gggcgatgct ggcagaagcc
     4681 cagcgactgc gggcgcaagg gctggatatt gtggttggcg tggtagaaac ccacgggcga
     4741 aaagataccg ccgccatgct ggaagggctg gctgttctgc cgttaaaacg ccaggcgtac
     4801 cgtgggcggc atatcagcga gtttgatctc gatgccgccc tcgcccgccg cccggcgctg
     4861 atcttaatgg acgaactggc gcacagtaat gcgccaggtt cccgtcatcc caaacgctgg
     4921 caggatatcg aagaactgct ggaagctggc attgatgttt tcactaccgt caacgttcag
     4981 catctggaaa gtctgaatga tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc
     5041 gtgcccgatc cttttttcga tgccgccgac gacgtggtgc tggtggactt gcccccggac
     5101 gatctgcgcc agcggctgaa agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc
     5161 attgaacatt ttttccgcaa aggtaatctg atcgccctgc gcgaactggc actgcgccgt
     5221 actgccgatc gcgttgatga gcaaatgcgc gcctggcggg ggcatcctgg cgaagagaaa
     5281 gtgtggcaca cgcgcgacgc gatcctttta tgcatcggcc ataacaccgg cagcgaaaaa
     5341 ctggtccgcg cagcggcgcg gctggcgtca cggctgggta gcgtctggca cgcggtgtat
     5401 gttgaaaccc ctgccctgca ccgcttaccg gaaaaaaaac gtcgggcaat tctcagcgcc
     5461 ttacgtctgg cgcaggaact gggcgcggag acggcaacac tttctgatcc agcggaagag
     5521 aaagcggtag tgcgttatgc ccgtgaacat aatctcggca agattattct cggtcgcccg
     5581 gcctcgcgcc gctggtggcg tcgggaaacg tttgctgacc gactggcgcg catcgccccc
     5641 gatctcgatc aggtgctggt cgcgcttgat gaaccacccg cccgcacgat taacaacgcg
     5701 ccggataacc gctcttttaa agacaagtgg cgtgtacaaa ttcagggatg cgtggttgcc
     5761 gccgcgttat gcgccgttat caccttaatt gccatgcagt ggctgatggc gtttgatgcc
     5821 gccaacctgg tgatgctgta tctgcttggc gtggtggtgg tggcgctatt ttatggacgc
     5881 tggccttcag tggttgccac cgtcattaat gtagtgagtt tcgatctctt ttttatcgcc
     5941 ccacgcggca cgctcgccgt ctctgatgtg caatatctgc tgaccttcgc ggtgatgtta
     6001 accgtcgggc tggtgatcgg gaaccttact gctggcgtgc gttatcaggc gcgggtagcc
     6061 cgttaccgcg agcaacgcac acggcactta tatgaaatgt cgaaagctct ggcggtgggc
     6121 cgcagtccgc aggatatcgc tgccaccagc gaacaattta ttgcctccac gtttcatgcc
     6181 cgcagtcagg tgttgttgcc cgatgacaac ggtaaattgc agccgttaac acatccgcaa
     6241 ggaatgacgc cgtgggacga tgccatcgcg cagtggagtt ttgataaagg cctgcctgcg
     6301 ggcgcgggca ccgacacgtt acccggtgta ccgtaccaga ttttgccgct aaaaagcggc
     6361 gagaaaacct acgggctggt ggtggtggag ccggggaatc tgcgccagtt gatgatcccg
     6421 gaacagcagc gcctgctgga gacgtttacg ctgttagtcg ccaatgccct tgagcggctg
     6481 acgctaaccg ccagcgaaga acaggcgcgg atggcaagcg aacgtgaaca gatccgcaac
     6541 gccctgctgg cggcgctttc gcatgattta cgcacgccgc ttacggtgct gtttggtcag
     6601 gcagaaatct taacgctcga tctggcaagc gaaggatcac cccacgcccg ccaggccagc
     6661 gagatccgtc agcatgtgct gaacactacc cgactggtga ataatctact ggatatggcg
     6721 cgaattcagt ccggcggctt taatttgaag aaagagtggt taacgctgga agaagtagtc
     6781 ggcagcgcgc tgcaaatgct ggaaccgggt ttatcgtcgc ccatcaatct ttctctgcca
     6841 gaaccgctga ccttaatcca cgttgacggg ccactctttg aacgggtgct gattaatctg
     6901 ctggagaacg cggtgaaata tgcgggtgcg caggccgaaa ttggtatcga tgcccacgtt
     6961 gagggcgaaa atctacaact ggatgtctgg gataacggcc ccggtcttcc gccaggccag
     7021 gagcagacga tatttgataa gtttgctcgc gggaataaag agtcggcagt accgggggta
     7081 gggcttggac tggcaatttg tcgggcgata gtggatgtac acgggggcac tattaccgcg
     7141 ttcaaccgac cggaaggtgg tgcctgtttt cgtgttacac ttccccagca aactgcccct
     7201 gaacttgaag aatttcatga ggatatgtga caaacgttct gattgttgaa gatgaacagg
     7261 ctattcgtcg ctttctgcgc acggcgctgg agggcgacgg gatgcgcgtc tttgaggccg
     7321 aaacgctgca acgcggcttg ctggaagcgg caacccgtaa gccagatttg attattctcg
     7381 atctcggcct gcccgatggt gatgggattg agtttatccg cgacctgcgc cagtggagcg
     7441 cggtgccggt gattgtgctt tccgcacgca gcgaagagag cgacaaaatc gccgcgctgg
     7501 atgccggagc ggatgattat ctgagtaagc cgtttggcat tggcgaattg caggcccgtc
     7561 tgcgcgtcgc attacgccgc caatctgcca ccaccgcgcc cgatccgctg gtaaaatttt
     7621 ccgatgttac cgtcgattta gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc
     7681 tcacaccaat tgagttccgc ctgctggcgg tgctgctcaa caatgccgga aaagtactca
     7741 cccagcgcca gctccttaac caggtgtggg ggccaaacgc ggtcgaacac agtcactatt
     7801 tgcgtattta tatgggacat ctgcgacaaa aactggaaca ggatcccgcc cgcccacgcc
     7861 atttcattac t
//
LOCUS       DAZFZC010000161         7817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFZC010000161 DAZFZC010000000
VERSION     DAZFZC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.163"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..145)
                     /locus_tag="ACHKXP_004665"
     tRNA            complement(70..145)
                     /locus_tag="ACHKXP_004665"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Ile,seq:cat)
     gene            complement(195..1244)
                     /locus_tag="ACHKXP_004666"
     CDS             complement(195..1244)
                     /locus_tag="ACHKXP_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7649512.1"
                     /translation="MINTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWEGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVEQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGISQWQLPNEGDYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSELNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(1395..1592)
                     /locus_tag="ACHKXP_004667"
     CDS             complement(1395..1592)
                     /locus_tag="ACHKXP_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7649513.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(1905..2447)
                     /locus_tag="ACHKXP_004668"
     CDS             complement(1905..2447)
                     /locus_tag="ACHKXP_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO7649514.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGSKTGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCYATCRNRIDMWL
                     RIAEFILYPLMRDAFSFTDA"
     gene            complement(2456..2821)
                     /locus_tag="ACHKXP_004669"
     CDS             complement(2456..2821)
                     /locus_tag="ACHKXP_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7649515.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGVKIYKIESE"
     gene            complement(2822..3877)
                     /locus_tag="ACHKXP_004670"
     CDS             complement(2822..3877)
                     /locus_tag="ACHKXP_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7649516.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(3879..4157)
                     /locus_tag="ACHKXP_004671"
     CDS             complement(3879..4157)
                     /locus_tag="ACHKXP_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649517.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(4325..4480)
                     /gene="hokD"
                     /locus_tag="ACHKXP_004672"
     CDS             complement(4325..4480)
                     /gene="hokD"
                     /locus_tag="ACHKXP_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7649518.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            5038..6135
                     /locus_tag="ACHKXP_004673"
     CDS             5038..6135
                     /locus_tag="ACHKXP_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO7649519.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFISPEDHYPIINAVNQVRD
                     MGIRVIPVSSPTRPSNYKRAIDELMQNEICLRLTTLDLVNPQLITHYINHLGIPLSNI
                     DIIIDLRDELTEDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDISVGL
                     YSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDNDF
                     WVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSGN
                     YKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
     gene            complement(6095..6673)
                     /locus_tag="ACHKXP_004674"
     CDS             complement(6095..6673)
                     /locus_tag="ACHKXP_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFO7649520.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(6979..7290)
                     /locus_tag="ACHKXP_004675"
     CDS             complement(6979..7290)
                     /locus_tag="ACHKXP_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649521.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(7283..7537)
                     /locus_tag="ACHKXP_004676"
     CDS             complement(7283..7537)
                     /locus_tag="ACHKXP_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649522.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            complement(7534..>7817)
                     /locus_tag="ACHKXP_004677"
     CDS             complement(7534..>7817)
                     /locus_tag="ACHKXP_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7649523.1"
                     /translation="RELVASGDVYNSGYGLFPSEQARKDWQNTRKKLSRAKLKKPSAV
                     DPDLIWSLPDGEIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
BASE COUNT         1996 a         1972 c         1673 g         2176 t
ORIGIN      
        1 ccgcccccgc accaacaacc ctgttatggt gtcgtctgct cttcctgata agctaatggc
       61 ggtttgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta tgagtcgctc
      121 gctctcacca ctgagctaaa gggccgggag cagaataata atggtgcgta attaattctg
      181 caatctcatc cgtttcaaac gattaaatcc tgaacttccc tgactgtctg ttcaaagcgt
      241 ccggtctcca gctcaacacc aatcgcacaa cgccccagtg ccatcgccgc ttttaccgtt
      301 gaacctgaac ccataaaaaa atctgcaacc aggtctcccg gacgactgct cgcgttgatt
      361 atctgctgca gcatttctgc cggtttttcg cacggatgtt tccctggata gtactgcacc
      421 ggtttatgcg tccagacatc ggtgtacgga acctgcgccg tcacaccgaa ataccgccgc
      481 aaatttttat attcactcag cagttccgta tactgccggt tcagctcact gtatgtgctg
      541 accagctggt ggtgtggctt ttccagttcc ccgcgctgat gtttttctgc cgcaacacgc
      601 gcaaacaacg cctgcaattt gttgtaatca ccctcgttcg gtaactgcca ctgactgata
      661 ccaaaccagt gcgaagccat gtttttcttt ccggtggctt ccgctatctg ttttgacgtt
      721 attcccagtg atttacgcgc atcacgaaag taagaaatca gcggggccat gacgtgctgt
      781 tttagctcgc gcccctgctc cacatagcca tcatctttcg ggcgatacgg tccctgataa
      841 tgttctgcaa acagaatgcg ctctgttgcc ggaaaatacg cccgcagact ttccttattg
      901 cacccgttcc agcgtccgga cggcttcgcc cagataatgt ggttcagcac attaaagcgc
      961 tcacgcatca tgatttcggt gtcagatgcc aggcgatgac cacagaacag gtaaagactt
     1021 ccggcaggct tcagtacccg ccagaactgc gccagacact ggtccagcca tttcaggtaa
     1081 tcatcgtcgc cctcccactg gttatcccag ccctcgggct tcactttaaa gtatggcggg
     1141 tctgtgacta tcagatcgac agagttttcc ggtaaggtct ggataaattc caggcaatca
     1201 gcgttgatta actcacaact ggatattttt acagtattaa tcatagatca ataagcactt
     1261 ctctgatagg ctcatgccgc ttttgcgcaa agcagatggg cctgaggttt gcttgtgacc
     1321 ccaacgcatg agcagatggc tggcaggtgc cgctaacacc caccagccgc ccattaccac
     1381 aaattaaaaa gccttcactg cggaaggcgt ctgtaacaac cgaactgata atctgccaga
     1441 cccgccataa caagctgagt cagtattaac tggcagcgtt cgcgtgaaag gtaagtattc
     1501 tgcgcaattt ccccgacggt cgccggttcg gtgacgctta attcattaaa caccactctg
     1561 gcggtttcgg tcatatcctg ctgttttagc atgccttttt cccttttctg gttaacgtga
     1621 cataccaata cctcttgtcg aaaaagccag caagctgaaa gaccagtatt cacaactacc
     1681 agcgcgttta atgttctgtg ccgtttttca ggcataaaaa aacccgcata aagcgggttc
     1741 tttcaggtgt ccatgtctgc tattcgcctc gcggtacagc tttgcgaagc gtagctggat
     1801 tgaaacagtt tatggctaaa aatacaagct ttttttctaa aactgcacaa accttactac
     1861 cagccaaaaa tcctcttcgt gcaacaacaa acgccctcca gattctaagc gtcagtaaaa
     1921 gaaaatgcat ctcgcatcag tggatacaga ataaactcag ctattctcag ccacatatct
     1981 atacgattgc ggcatgttgc atagcaccac tcagggtgaa cctcattcaa caattcagcc
     2041 attttgcgtt tactcatccc ccgcccttcg tatctttgcc gcaggatatc aatcaatcca
     2101 ggataacgtg caagcgcttt acttatcacc ccatcaatgc gtaacgcctc tgcatcagta
     2161 cagtgagaca accagctctt ctgtctgcca gcgatcatct ctcgcaagaa tgcttccagc
     2221 tctggtttat caatccctga ctccctgatt ctacgcaggg cttcattgat tgcggttttt
     2281 gtcagttttt tggatgccag caactgattg aacatatttc ctgttttgct accacctata
     2341 tacgaccaac gcccccacat ccgtaatttc ccctggatcc agacggcttc cagcgttttt
     2401 agacgtaaat gctcgccgct tttgcctgta atttccgggt atatcatatt tacgatcact
     2461 cactctcaat tttgtaaatc ttcacgccca gccgcccacc aggaacgagc tgaccgcgca
     2521 caatattgat ttcatcaaac tgctcgtcgt ctatgagaag tccggcatgc gtcaacgcat
     2581 ccagtggtgc tttcaggata ttgtccaggt cacggcggcg cttatccggt ggttctgcaa
     2641 taatttttat tgccaacctt ccggacaggc ttaatttcag ccgctgctgg cgaacaataa
     2701 gcgccactgc ccggcgataa cgctccccgg cttttgatac aaaatatgtg ctgccacgac
     2761 gacgccagta agtgttcacc gttggcgggt aaggcaaaac aaattctatg cgttcagtca
     2821 tttatgcttt ccacttcaga acacccgaat ttctcgcgtg cattaaaaaa cgaatcagca
     2881 acaacagctg gctgccgtgt ttttcttcaa aatcttttac cccggcgtgt agttcgctat
     2941 ggcatttacg gcacagcgga ataacaaaca aatcatcagc ctttgttccc atccctccca
     3001 gtccatgacc aatgatgtga tgcggatcat ctgcctgatt gccacacgtc atgcatttct
     3061 gcgtttttac ccagcgcgtg tatacaggca tctcttcccg ttgtgatttc tggcgctgga
     3121 gatactgagc cggtgactcg ggatcaacgg caatgctgac caccgtcttt tcctgtggcg
     3181 ggttttgctg gtgggcgtga ggcagcggcg caagattttt tgtgcgctgc ttcagtatgc
     3241 tggtggcggt ctgctctccc ggtacgatgt cgctttcacg gtacattgag cggatttttt
     3301 ccgcacgcaa ccccagcgaa cgacgtaata ccgcttccgg tagcgcgtcc gccacctgat
     3361 tgcggaccgc ccaccaggat aattcagcca gcgataattc ccgttcctgc gagccattca
     3421 ttgcatggcg tatgacgtca atcatccatg cagacaggtt ttggtgagca agttgcccga
     3481 gtgattcgga ggtctggtcg cgcagctggt tgtcgcagtg ccagcacaac accattgcgc
     3541 cggtaccata acggtgaatg acggtttcac tgtggtgata atcaccgtgt ggccactggc
     3601 aggatttaac atggcgcagt aaccagtcag acaatgcgcc agcgccacca gcagcacgaa
     3661 tcactcgttc gtcgctgaaa aatggcagta atgatttatc ctccgccagc ggctggcgaa
     3721 cggcaggaac gaccccggac ggcagattac gcatgctttt cggttccggc tccaccagta
     3781 cccgggtatt gtggaatacc ggcatggatt cacggcccgg cttaacgatc accagcccga
     3841 gttccggtac cagaacaggt cgaagtaata cccgcacgtt acctccagat gcgttgctgg
     3901 aatgtgcggg acggacgcgg tggtcgttcg gagtaaggaa gcctgacgga gattatccag
     3961 tgacgataat cgaggctgag ggctttctta atctcgtatc cgtgtctgcg gtagcactga
     4021 attagccact cggcctgttc ttcagtgcat gggggatgct ggaaccagtc agatttgaaa
     4081 gtgcgggaac gccgcccgtg cctgctggca ggggcggcag agttatccga attgtaaaat
     4141 ttggtatcgt gcgccatctg ttttctctgc tggcgcagca ggtgccagtt gttcaggctg
     4201 acggatggat tgtaaaccag aacgaccaga aaaaacaaaa cccgccgaag cgggttaagt
     4261 gcgggtgcgt tgaggatgcc tgactcatca gaggtggcga gggatttctc cctcgcctgg
     4321 tctcttactc ctcaggttcg taagctgtga agacagcgac ctccgtctgg ccggttcgga
     4381 ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt cactatgacg gttaaacaga
     4441 tgacgatcag ggcgattaac atcgcctttt gctgcttcat agcctgcttc tccttgcctt
     4501 tcggcacgta agaggctaac ctacatgtgc aaagcatgaa attggcctca gattaatgtt
     4561 aagcgtcttg ccggacgcgt aatgttaact ggggcttttc tctatctgcc ttttggtgtt
     4621 catgcctgag gcagatagcc tcaagcaccc gcagcaattc taactatcca atagatcaat
     4681 gccaacttct tttcctgcaa catctctcat cagaagtgac ccaaattcat ccaatcagca
     4741 caaaagaaac atttaaagcg tgaatgctac cgaacgttca tttcaattac atcgttttga
     4801 tttttaacag ttttcacctc aacaaatcaa atggcattag cattcatcat ggaagaaaga
     4861 ctgataaaaa tgatcatttt caatgactta tgatcacacc gggtattgcc aagtcattcc
     4921 ttttacgcca ggatacccac aagtgagtag tcgaggagag tttgccaatt tttgcgatat
     4981 aaatttcaat gtccgaagaa ccattaattg ttaattgatt ctgtataagg tattaacatg
     5041 acaatttcat atattcctat tctgaaagcg aaacgttctg agttatcagc tttatcgcag
     5101 ctatccattg aaaagaaatc aaaaattttg ccattactcg aaattgaacc agtgccaatt
     5161 gaccctgatt caggtattgc cttaaagagt tataacgaga ctcttataga gttcggaaag
     5221 aaagtctcaa aatcttgctc agatatgcaa ggtgtttata ttgatggatt attaattgaa
     5281 gagcatttta tttctcctga agatcattac cctataataa atgcggttaa tcaagttaga
     5341 gatatgggga taagagttat tcctgtcagt tcaccaactc gcccatctaa ctataaaaga
     5401 gcgattgatg aattaatgca gaatgaaata tgcttgagat taaccacgtt agatctggtt
     5461 aacccacaat taataacgca ttacattaat catctgggaa ttcctttatc aaatattgat
     5521 ataattattg acttaagaga tgagttaacc gaggataaaa ttaattccgg cgaactatat
     5581 actttggcaa tgggattgat aaacaatctg gcgcacctca atgaatacag aaaagtgatt
     5641 ctttctgggg gttcatttcc tacagatctc agtgatattt ctgttggtct atattctcaa
     5701 cctcgaatcg agtggatttt atggcagagt ttaatgaata gaaaagaact tgccagaaat
     5761 gtgatttata gtgattatgg agtacagcac cccgacttta ataggctttc gacgcgattc
     5821 cctagcgtat ctgccagcgt cagatactct ggagataatg acttttgggt atttcgagga
     5881 agagtagcta atcgctttgg ttatgaacaa tatggtaaac atagtgagga tattcttgct
     5941 catcgagaat attcagggcc tacattctgt gcgggagata gagacataga atattatgca
     6001 aacgagtatc aagcctacaa agccaatccc tctggtaact ataaatttgg tagcccagag
     6061 gtatggcgta ggattgggca aaaccatcac ataactaagg ttgttgagca actcgccact
     6121 ctttacgggc tttaagttta actcgaacag cttcacgaag ctcgccaaca tccatactat
     6181 tggcgagttt tgaccataag acgcgtttag gtttattttt caaattctgt agctccccta
     6241 catcacttaa tagggaaagc aattcatctt tccaaagaag cattgttaag gaaagtttgt
     6301 ccacttgtgg attcaacttg ctagttcgaa ttgtttttaa atggatacct tgccgtgcac
     6361 cttgcgtaac ttgctttatc ccccaccatg atggaactat gcttaaagca tcataaagat
     6421 ggcaatcaga gacaaccaaa gttactttat ccatcactga tgagtaatgt tgcacctgcg
     6481 ctggtaatct gagcaagtta tcactcttgc tcttcaactc ataaccatgt ataagcccat
     6541 tgatcactgc tatatcagct ctgctagccc ctagattcat cgtaaactca tcaatgatta
     6601 gggtgtcagg atctttatga tgatctctca gaatcttggc atgcacagcc tttcttacat
     6661 caatgtctct cataccatag ttgcgctttc atcgactctc tcaagccatg attctatatc
     6721 atggttcgca tgaatgccat ttgatatgac cagtggttaa acattgcaca gaaaagtgga
     6781 tgtgtatttt aacaaaagca atgacggcta accatgttgg tatgtcgata aaatgtttca
     6841 acgtaagcat tatctgttcc gccctttcag acggcctcct gatgttctga gggtgcagaa
     6901 tccctccggt taaggattta ataaaaatcg tttctgattt aaatcttcag tatttagttg
     6961 ttagtcggtt tatagccttt atgcttcggc cttatttctc agccatacac aaaccgggcc
     7021 atcttcggtg tcatgtattg aaccaataaa ccatccattg ccctctggtc gttccggttc
     7081 ccatgcagaa atatcagcat cacacgcatc aaggtcagca catccttcat ctctgaagca
     7141 gaggacgtat tgaagattat tttcctccat ccaggcgtta aactcttccg ttgaaatata
     7201 ttcccgaccg tcacagaatt tttcatattc aggatgcgtc cagcagccat attcatcacg
     7261 tactactggt atttctttaa tttcattcat ttctgttctc ccacgttttc agactttcac
     7321 cacagaacgg acaaaatgaa acccgaactg gtaatttaga aaattcaccg gaacgcaaca
     7381 tcacaaaatc aggaccgcga gttaaactct cattccagat tttgtatatc agcagacctt
     7441 ttcgcatcgt gtattcagca tcatgctcaa gggactttgc cagtgctgca catggttcta
     7501 tcttgttgcc attaacctgg cattttgatt cactcaccgc accacctcct caaaattccc
     7561 ctgataaaac gccagtacgc gctgcataac ttcgctcttc cggcactcga gacagattat
     7621 gttcagacgc ctgtcgtagc gacgtatttc tccgtcaggt aatgaccaga taaggtccgg
     7681 atcaaccgca gatggtttct tcagctttgc ccttgagagc tttttacggg tattttgcca
     7741 gtccttacgc gcctgttcag acgggaataa cccgtaacca gagttgtata catcgccact
     7801 ggcaaccagc tctctgg
//
LOCUS       DAZFZC010000162         7755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFZC010000162 DAZFZC010000000
VERSION     DAZFZC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.164"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..66
                     /locus_tag="ACHKXP_004678"
     CDS             <1..66
                     /locus_tag="ACHKXP_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7649524.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
     gene            366..1532
                     /gene="sopA"
                     /locus_tag="ACHKXP_004679"
     CDS             366..1532
                     /gene="sopA"
                     /locus_tag="ACHKXP_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO7649525.1"
                     /translation="MRLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            1532..2503
                     /locus_tag="ACHKXP_004680"
     CDS             1532..2503
                     /locus_tag="ACHKXP_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO7649526.1"
                     /translation="MKRAPVIPKHTIKTQPLEDTPSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQRAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRIPTECIERIEAILKELEKAAL"
     gene            2968..3137
                     /locus_tag="ACHKXP_004681"
                     /pseudo
     CDS             2968..3137
                     /locus_tag="ACHKXP_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3134..3481
                     /locus_tag="ACHKXP_004682"
     CDS             3134..3481
                     /locus_tag="ACHKXP_004682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649527.1"
                     /translation="MMTMAGKIPAFFLPVPGQSTKEVAAGSALPGHAEHGLVGVQLCG
                     RRVVCGLCRASILAGLALMQATVLMGGGYLKKVRDNIMESPISSKKLTRFDTTLTGCF
                     ERDISRVGHRHFK"
     gene            3550..4125
                     /locus_tag="ACHKXP_004683"
     CDS             3550..4125
                     /locus_tag="ACHKXP_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilome CxxCx(11)CxxC protein"
                     /protein_id="HFO7649528.1"
                     /translation="MDEEKTRAEVAHIEFISYGTSRIFDRRMRSLDWKRKVVTFLGVF
                     VPLMIGCAVLSFGLEAPFLPLCITIAGVASIMQLGFSLWSLVSGWDRSYSDCMASVKE
                     NTAIYNLAGSVRKKIGKLDEAKLEILIDDLTEKFERREQEDLTLCVSDKELRYANRMS
                     CFYFKKKCHICNVVPLTLKPGKCVCDGCGKF"
     gene            4138..4350
                     /locus_tag="ACHKXP_004684"
     CDS             4138..4350
                     /locus_tag="ACHKXP_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649529.1"
                     /translation="MSSAKILNAYFEASEEDRKEFLRVLSHFYKDNRRVPLQEGMESF
                     AVNESIRKSQSINFAPMQGGCPLCGK"
     gene            complement(4611..5582)
                     /locus_tag="ACHKXP_004685"
     CDS             complement(4611..5582)
                     /locus_tag="ACHKXP_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc32 family transposase"
                     /protein_id="HFO7649530.1"
                     /translation="MSQPNLQCMGIDVAKLSLDIATTDTIEPFTVGNDEDGFAVITDK
                     LKHTKINLILMEATGGLEAAIACKLQSEGYDVVVINPRQARDFARSMGYLAKTDKLDA
                     AMLAQLALVIDRHPDRSRYIRHLPDEARAVLAAMVVRRRQLNHMLVAERNRLYPSHPQ
                     SRKSIDNIIDALQNELDRINEQMKQHMTAFFQEQARLIGSVKGVGDITVASLIAELPE
                     QGKLNRREISALTGVAPLNRDSGKMRGKRTTFGGRAGVRATLNMAALVATQFNPAIKL
                     FYQRLLAAGKPKKLALVACMRKLITILNTMLRKGEEWNASFQSQVIS"
     gene            5984..6409
                     /locus_tag="ACHKXP_004686"
     CDS             5984..6409
                     /locus_tag="ACHKXP_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7649531.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGNAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            6409..6870
                     /locus_tag="ACHKXP_004687"
     CDS             6409..6870
                     /locus_tag="ACHKXP_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7649532.1"
                     /translation="MNGRGGNAAPVKETRTCTVKEIIRDVLDTDVPDSECVFAVVLTR
                     GNVRHMAQDWNLSDDELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPA
                     VLLEKVMVMAGSEIYRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            6870..7052
                     /locus_tag="ACHKXP_004688"
     CDS             6870..7052
                     /locus_tag="ACHKXP_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649533.1"
                     /translation="MNISTETREILRNYKTVINARRREMGQKPLTTAQIVDEICDFVT
                     NQQAVFLGGHYILQGS"
     gene            complement(7276..7529)
                     /locus_tag="ACHKXP_004689"
                     /pseudo
     CDS             complement(7276..7529)
                     /locus_tag="ACHKXP_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7526..>7755)
                     /locus_tag="ACHKXP_004690"
     CDS             complement(7526..>7755)
                     /locus_tag="ACHKXP_004690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649534.1"
                     /translation="SLWYELHPFENLRRKGHHCHGLTHLSWAERKIFYRVLGKKFCGL
                     FIYPGQSVRQVVAVSAPGDRDSYGCVALGRQ"
BASE COUNT         1837 a         1804 c         2023 g         2091 t
ORIGIN      
        1 tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat
       61 caataagttg gagtcattac cgccttgacc atcagatagt tattttggtg ctagggatac
      121 tgagtgtgaa cagttagcgc gcgtcctcgc cagttagctc gctacgctcg gttacacgac
      181 ggctacgagt actagtgata attattgact gaggtatgtg ctcttcctgt ttcctttttt
      241 gatgtgttgc tctcatttta aacaactttg cggttttttg actactttgc aattttgttg
      301 ttgctttgca ataaaatgca agattaaatg aaaatatgca aagcaatgat taaaggatgt
      361 tcagaatgag actcatggaa acacttaacc agtgcataaa cgctggtcat gaaatgacga
      421 aggctatcgc cattgcacag tttaatgatg acagcccgga agcgaggaaa atcacccgac
      481 gctggagaat aggtgaagca gcggatttag ttggagtatc ttctcaggct atcagggatg
      541 ccgagaaagc aggccggcta ccgcacccgg atatggaaat acgaggacgg gttgagcaac
      601 gtgttggtta tacaattgaa caaattaatc atatgcgtga cgtgtttggt acgagactac
      661 gacgtgctga agacgtattt ccgccggtga ttggagttgc tgctcataaa gggggcgttt
      721 acaaaacctc tgtttctgtt catcttgctc aggatctggc tctgaaggga ttacgtgttc
      781 tgctcgtgga aggtaacgac ccccagggaa cagcatcgat gtatcacggc tgggtgccag
      841 atcttcatat tcatgcagag gatactctcc ttcccttcta tcttggggaa aaggacgatg
      901 tcacttatgc aataaagcct acttgctggc ctgggcttga cattattcct tcctgtttgg
      961 ctctgcaccg cattgaaact gagctaatgg gcaaatttga tgaaggtaaa ttgcccaccg
     1021 atccacacct gatgctccga ctggccattg aaaccgtcgc tcatgactat gatgtcattg
     1081 tcattgacag cgcgcctaac ctaggtatcg gcacgattaa tgttgtatgt gctgctgatg
     1141 tgttgattgt ccccacgcct gctgagttgt tcgactacac ttccgctctg cagtttttcg
     1201 atatgcttcg tgatctgctc aaaaacgtag atcttaaagg attcgagcct gatgtacgta
     1261 ttttgcttac caaatacagt aatagtaatg gttctcagtc cccgtggatg gaggagcaaa
     1321 ttcgggacgc ctggggaagc atggtcctaa aaaatgttgt gcgtgaaacg gatgaagttg
     1381 gtaaaggtca gatccggatg agaactgttt ttgaacaggc tattgatcaa cgctcttcaa
     1441 caggtgcctg gagaaatgcc ctttctattt gggaacctgt ctgcaatgaa attttcgatc
     1501 gtttgattaa accacgctgg gagattagat aatgaagcgt gcgcctgtca ttccaaaaca
     1561 tacgattaag actcaaccgc ttgaagatac tccgtcatcg gcaccagctg caccgatggt
     1621 ggattcgtta attgcgcgcg taggagctat ggctcgtggc aatgctatta ctttgcctgt
     1681 atgtggtagg gacgtgaaat ttacccttga ggtgctccgg ggagatagtg ttgagaagac
     1741 ctctcgggta tggtcaggta atgaacgtga ccaggaactg cttaccgaag acgccctgga
     1801 tgatcttatc ccttcttttc tactgacagg tcagcagaca ccagcattcg gtcgaagagt
     1861 gtctggggtc atagaaattg ccgatgggag ccgccgtcgt aaagctgctg cacttacaga
     1921 aagtgattat cgagttctgg ttggtgagct ggatgatgag cagatggctg cattgtccag
     1981 attgggtaac gattataggc caacaagtgc ttatgaacgt ggtcagcgtt atgcaagccg
     2041 attgcagaat gaatttgctg gaaatatttc tgcgctggct gatgcagaaa atatttcacg
     2101 taagattatt acccgctgta tcaacacagc taaactgcct aaatcagttg ttgctctttt
     2161 ttctcatccc ggtgaactat ctgcccggtc aggtgatgca cttcaaagag cttttacaga
     2221 taaagaagaa ttacttaagc agcaggcatc taaccttcac gagcagaaaa aagcaggagt
     2281 gatatttgaa gctgaagaag tcatcactct tttaacgtct gtgcttaaaa cgtcatctgc
     2341 ctcaagaact agcttaagct cacgacatca gtttgctcct ggagcgacag tgttgtacaa
     2401 gggcgataaa atggtgttaa acctagatag atctcgtatt ccaactgagt gtatagagag
     2461 aattgaagcc attcttaagg aacttgaaaa agcggcactt tgatgcggca cgttttagtc
     2521 tacatttatc tgtctttact taatttcctt tagcacaggc tagaaaacat aactaactgg
     2581 cctgaatatt atatctggga ccacggtccc agttgtattg tcggtctgat aatcagtctg
     2641 ggaccacggt cccactcata ttgtcggtct gataatcagt ctgggaccac ggtcccactc
     2701 atattgttgg tctgctaatc agtctgggac cacggtccca ctcatattgt cggtctgcta
     2761 atcagtctgg gaccacggtc ccactcatat tgttggtctg attatcggtc tgggaccacg
     2821 gtcccactca tattgttagt ctgataatca gtctgggacc gcggtcccac tcgtattgtt
     2881 ggtctgctaa tcagtctggg accacggtct cacttgtatt gtcgatctga ttatcagcgt
     2941 gagactacga ttccattaat gcctgccagt gagtgtgtgg aacatatctt ggccagtggc
     3001 ttggtctgtg atgtgctgcg tataccgggt gaaccgcccc gccgcttgtt tgaccgctat
     3061 attctgcggg agttttcttg gaggcacggg atgaaatccg cagcatggcg gatgccctgg
     3121 caaaaatccg gaaatgatga cgatggcggg gaaaatcccc gcctttttcc tgccggtccc
     3181 cggacagagc acaaaggaag tcgccgccgg ctccgcttta cccggccatg cggagcatgg
     3241 ccttgtgggg gttcagttat gtggacgtcg agtcgtgtgc gggctgtgcc gtgcctccat
     3301 cttagccggg ctggcgttga tgcaagcaac tgtcttaatg ggaggcggtt atctgaaaaa
     3361 agtacgggat aatattatgg aatcgcccat ctcaagcaaa aaactgactc gcttcgatac
     3421 tacgctgaca ggctgttttg agcgagatat ctctcgtgtc gggcatcgac attttaaatg
     3481 attctaaata atatgagcag ggtcagtctt attaaagatt aaaacaccgg ataacaagtt
     3541 ttattgatta tggatgaaga gaaaactcgt gctgaagtgg cacacataga atttatttca
     3601 tatggtacgt ctcgaatttt tgataggcgt atgagatcct tagactggaa gcgtaaggtt
     3661 gtcaccttcc ttggggtctt tgttccactg atgataggtt gtgcagtttt atcattcgga
     3721 cttgaagctc catttctgcc gctttgcatc acaattgccg gtgtggcaag tatcatgcaa
     3781 ttggggttct cgctgtggtc tttggtttcc ggatgggatc gctcatatag tgattgcatg
     3841 gcttccgtta aggaaaatac agccatttat aacctagctg gtagtgttcg caagaaaata
     3901 ggtaagcttg acgaagccaa attggaaatt ttgatagacg atttgacgga aaaatttgag
     3961 cgaagggagc aggaagactt aactttgtgt gtgagtgata aagaacttcg ttatgcaaac
     4021 cgtatgtcct gtttctactt caaaaaaaaa tgccatattt gtaatgtcgt tcccctaact
     4081 ttgaaaccgg gaaaatgtgt ttgtgatggt tgtgggaaat tttaacaagg agaacatatg
     4141 agtagtgcaa aaattcttaa cgcttacttc gaggcttctg aagaagatcg aaaggagttc
     4201 ctacgggttc ttagtcattt ttataaggat aaccgaagag tacctctgca agagggcatg
     4261 gagagctttg ccgtcaatga aagcatccgt aaatcgcagt caattaactt tgcccctatg
     4321 caaggtgggt gtccactctg cggcaagtaa cgaccaaagc cgcttgacta gcggcttgat
     4381 agatttcttt atattgtaac tagcatcttt acatcctgtt aaaaaagtgg ctattgcccg
     4441 ctatcttgca attctccgta tttggtacaa aaacagccga aagctgcact atcagcagct
     4501 atgagcagcg gccccactga catcgcggat gctttgtccg tgggagggcg gatcacaaca
     4561 ggcctgcggg ataagttgtt ctttctgtca ttatgtaaaa tgaccggcag tcatgagatt
     4621 acctgtgatt gaaatgaggc gttccactct tccccttttc tgagcatggt attcagaatg
     4681 gtgatgagtt tgcgcatgca ggcgaccaga gcaagttttt tgggttttcc ggcggcaagc
     4741 aaacgctggt agaacagctt tatggcagga ttaaactgcg tagccaccag agctgccatg
     4801 ttcaacgttg ctctcactcc ggctctgcca ccaaacgtgg tccgtttccc tcgcattttc
     4861 ccggagtctc tgtttagagg agcgacgcca gttagagcac taatctcccg tcgattgagt
     4921 ttcccctgtt ccggtagttc ggcaatcagc gacgcgacgg tgatatcgcc gacgcctttc
     4981 acgctgccta tcagtctggc ctgctcctgg aagaatgctg tcatgtgttg tttcatttgc
     5041 tcattgatcc ggtcgagctc gttttgaagc gcatcaataa tgttatcgat actcttcctg
     5101 ctttggggat gagaaggata gagacgatta cgctcagcga ccagcatatg gttcaactga
     5161 cgacgacgga cgaccattgc ggcaagtact gctcgtgcct catctggcag atgccgtata
     5221 taacgactgc ggtccggatg gcgatcaatg accagggcca gttgtgctag catggcggcg
     5281 tcaagtttat ctgttttagc cagatatccc attgaacggg caaaatccct agcctgtcgt
     5341 gggttgatca caaccacatc gtatccttct gactgaagct tacaggcaat ggctgcttca
     5401 aggccaccgg tagcttccat gagaatcagg ttaattttgg tgtgcttcag tttatctgtg
     5461 ataacagcga aaccatcctc atcgttaccc acagtgaatg gctcaatcgt gtcggtggtg
     5521 gcgatgtcca gcgatagttt ggcaacatca atacccatgc attgcagatt tggttgactc
     5581 atatgaccct tccttacgat acgaatttgg tttcaatcaa ctgtacggat ttgtgatgag
     5641 tgactcagct catgcgcggg acgcttgcat gcgggcttaa taccctcggg ttcgcacgtg
     5701 ctgcatgagc tttgccagac ctacagattc agtttatgcc atcttcaaga tataaggata
     5761 cgcttcgccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt tccggtcgtt
     5821 tacccgctca gatttcgcgg tctcaccctc caactcccgc cagccgtgtg tgtcaggctg
     5881 cggcttccct tgcgcccttt gcatccccgc cttatcgccc ggcttttatg gaggcacggc
     5941 acagcccggt gtcgcggaat atgtgaacaa cggaggaaat cccatgcagt atgcgaaacc
     6001 tgtcactctg aatgttgaag agtgcgaccg tctgtctttt ctgccttacc tgtttggcaa
     6061 cgattttctg tatgccgaag catatgtgta cgcgctggcg caaaaaatga tgccggaata
     6121 tcagggaggc ttctggcact tcatccgcct gccggacggt ggcggttaca tgatgccgga
     6181 tggcgaccgt tttcatctgg ttaacggtga aaactggttt gaccgcacgg tcagtgccga
     6241 tgccgcaggc atcattctga cttcccttgt gattaaccgc cagttgtggc tgtaccacga
     6301 cagcggtaac gcagggctga cacacctgta ccggatgcgt gatgcgcagt tgtggagcca
     6361 catcgaattt caccccgaat gtaacgcgat ttacgcggcg ctggactgat taacggacgg
     6421 ggcggcaatg ccgcccctgt aaaggagacg agaacatgta ctgttaaaga aattatccgc
     6481 gatgtactgg atacagacgt gccggacagt gaatgcgttt ttgccgtggt gctgacccgt
     6541 gggaatgtgc gccatatggc tcaggactgg aacctgtcag acgatgagct ggaaaccgtc
     6601 atgcagcggc tggacgatgc ttttgtatat ggcgcatgtg acagagttgt ttccgatatt
     6661 gtcaatgaac tgatggagga aaagcgcgta aaccgccttg taacagtccc tgcggtgctg
     6721 ctggaaaaag tgatggtgat ggctggtagt gaaatttatc gcctccatgc tgtcgggagc
     6781 gagaacgggg gcgacggtga cgcatttgtc agggaagaac gtgagattat gcgggtgatg
     6841 cgtcaggcgc tggacgggga gaacgggtaa tgaatatcag cacggaaacc cgcgaaattc
     6901 tgcgtaatta caaaaccgtg attaatgcgc ggcgtcgtga gatggggcag aaaccgctca
     6961 ccaccgcgca gattgttgat gaaatctgcg attttgtgac gaatcagcag gcggttttcc
     7021 tcggtggtca ctatatcctt cagggcagct gaaacaggtg atacagggca ggcggtggag
     7081 gccgcctgtc agcccgcgcg aacccccctg gcaggctgac gcctgccgga agggaagcgg
     7141 gccacgctga cgcgtcgccc gttacccttc ccgttagtga tgtgtccgga atgttttttc
     7201 tgatgttatt tttctgtggt taccgtgttt gttattgttc ttcttctttt tcctcttcct
     7261 cttctttccg ttcctctcct gtttatggct tttccgggcg tccccctggc ggggcgggct
     7321 gtcgtgaacg gagccagcgg agctgtctcc gccctggcgg gcttccatcc ctgacgcgaa
     7381 accatggctt gcagcgaccg ttccggtcgc gccgcctctg tcgcgccgtg gcctgcgctc
     7441 ccggcatccc ggcaaaaggc gccggaatgt aaggagcaac gccgtttcgc aaaccgcgct
     7501 tcacgtcgtt gcggcgggtc cctgctcatt gcctgccaag tgccacacac ccgtatgagt
     7561 ccctgtcgcc gggggctgag acagccacaa cctgccgcac tgactgtcca gggtaaatga
     7621 acagcccaca aaatttttta cccagaaccc ggtaaaaaat tttgcgttct gcccaggaca
     7681 ggtgcgtcag gccgtggcag tgatgcccct tgcggcgaag gttctcaaac gggtgaagtt
     7741 cataccacag ggaat
//
LOCUS       DAZFZC010000163         7648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFZC010000163 DAZFZC010000000
VERSION     DAZFZC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.165"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2396)
                     /locus_tag="ACHKXP_004691"
     CDS             complement(<1..2396)
                     /locus_tag="ACHKXP_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO7649535.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNISGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEIVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKKQYLFNPPWESLADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGDV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFELKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQANPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRT
                     RVKTGVTYTYKDDKRHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRN
                     VEKIGNAECLCIMVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKP
                     GILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRY
                     G"
     gene            complement(2507..3238)
                     /locus_tag="ACHKXP_004692"
     CDS             complement(2507..3238)
                     /locus_tag="ACHKXP_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7649536.1"
                     /translation="MSMNLMAKAMNIKVGNPLRKLVLIKLADNANDNGECWPSYQHVA
                     DQCEVSRSTVKSHIRALEEMGLLKREFRRKGELNQSNVFYLTLDNAQQIQPESGGAGA
                     APRTYHSFEPVNEPKNIMFEHVRTECEKTPDRHEETDKAFEEIFWCAGMRKAGKKNAA
                     SAFRTQFREWRKTTRGTASEFATMLAEDIACRLGKQFGFDRLLPSSYLNGQRWNDEKP
                     ETIQPQSKPSSAITVSKTGYVFFDR"
     gene            complement(3401..3700)
                     /locus_tag="ACHKXP_004693"
     CDS             complement(3401..3700)
                     /locus_tag="ACHKXP_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO7649537.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSDQIQMEF"
     gene            complement(3839..4039)
                     /locus_tag="ACHKXP_004694"
     CDS             complement(3839..4039)
                     /locus_tag="ACHKXP_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7649538.1"
                     /translation="MEQRITLKDYAIRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEIKPFPSNKKTTA"
     gene            4140..4853
                     /locus_tag="ACHKXP_004695"
     CDS             4140..4853
                     /locus_tag="ACHKXP_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7649539.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            5071..5433
                     /locus_tag="ACHKXP_004696"
     CDS             5071..5433
                     /locus_tag="ACHKXP_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649540.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPCFRVDKTSEIAVIDKLLILASDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            5449..5577
                     /locus_tag="ACHKXP_004697"
     CDS             5449..5577
                     /locus_tag="ACHKXP_004697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649541.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
     gene            6365..6433
                     /locus_tag="ACHKXP_004698"
                     /pseudo
     CDS             6365..6433
                     /locus_tag="ACHKXP_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            6492..6857
                     /locus_tag="ACHKXP_004699"
     CDS             6492..6857
                     /locus_tag="ACHKXP_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649542.1"
                     /translation="MEEQANKILVELLQKASNGIDAAVSFSQAQIPDVIHQLLMWHAV
                     SSAGTQAICVLVIIACVYLMIFAWNKGDDADIVLLSLLVTSGIAITSIVVFFNHFDWL
                     KIWLAPKLYLIEYAASLVK"
     gene            6936..7406
                     /locus_tag="ACHKXP_004700"
     CDS             6936..7406
                     /locus_tag="ACHKXP_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649543.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            7556..>7648
                     /locus_tag="ACHKXP_004701"
     CDS             7556..>7648
                     /locus_tag="ACHKXP_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649544.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKL"
BASE COUNT         2062 a         1790 c         1675 g         2121 t
ORIGIN      
        1 ccgtatcggc ggcgagcgta gtcgaatatt tcgatcagcc tgtcggcttt cgccgttccg
       61 gtcaggccaa acacccaaag tctttcgtca taaaatttaa atgcagagtc aatttccagc
      121 actggcggca tcttgcagca cgtcgcctga cgggtaaggc gcttaaggag aataccaggc
      181 ttcagctcaa gtgacgcgat gcacgtcttc acaccctgac gcattgcctc aagtgccata
      241 tgcccgacaa cctcggtgtt atgcgtcgcc acatatcccc tggttacata caggtggcga
      301 gggtgatcga ccataataca caagcattct gcattgccga ttttttcaac attcctgacg
      361 aataccccac agccctcgaa tcgatgcgca gttaatctgc cattgagccg tggtgaacgg
      421 atggcctccc tgatttctct tgtcagtctg attcttgcct cgtatgaatc catcccatgc
      481 cgcttatcgt ccttgtatgt gtatgtcacg ccagttttaa ctcgcgtccg gcatgacccg
      541 ccgagtgagt taaccagttg aacaacccca ttgcgcagtt cttcacttgc tgaggaaaaa
      601 acaagcgttc catccttctc gacataccca tccgtttcaa gcagaccaca cagcatgcct
      661 atacgcgttg atttatttgc ggaaaaaaac acacgaggga tgaatttgtt ttttgctgtg
      721 caccccatta gtccataacc tcgcagggtc tccatgagtg gatttgcctg accacgcgcc
      781 gttgatatca gccagtcctt accatctcca gagaagttgt aatcaggcag ttcagccttc
      841 atacgctcga tcatgtatgg ctcgacgttt gaaaacttca cgctcccatt gctaagacta
      901 ccatccccga gaagggagcc gataacccat gctaatggct ctgagtggtc gccaaagtca
      961 ccagttattt caggaatcct aacgccattt ttgtgcctct tcgtttcact caaccgcttc
     1021 agctcgaagg tgtcaatcac gcggcgtttc tcgcctttcg tgaatccacg actagtgacc
     1081 tcccataggt gatcgcctgc gcaatcaaca taacgaccat cttcaaatgt gactcggtaa
     1141 acatctctaa caccctgcgg gaatatccca gtgacagttg acggattgcc gtctactgac
     1201 gccacctgat cgccaatttt tacatccccg tgagtagtcc atgtcccatc agctaaaaga
     1261 ataggctcat tcagtgggca tgcctttccg tgaccgttca caccattgac cagcgtcaac
     1321 tcggcctcac ggaactggaa tttatctgcc agagattccc acggtggatt aaacagatac
     1381 tgcttcttgc cgtagaaagc gttgatagtg tcctggtaaa actctcgcgc actgtagagt
     1441 tcttcaggat cgaaatagga tgccgtgccg atgtactgcc agatttcatc ctcggtaaca
     1501 ccgttcatca ggcattcgtt gatgtctttg tacggcagag taacaagacg gcaacgatgt
     1561 tcaccgagtc ggcttgcgat ttcccttgcg gcttcacgac caacatcatc aacgtccatc
     1621 gagatgaata tttcctcaaa cctgtcgagg ttgtgatact caaactcaat ccactgctgc
     1681 ttagcgcctt tcccgccacc aaacggcacg gataacgccg agatgccgta ttgcgcatag
     1741 ctcatacaat caatttcgcc ttcgcaaagt acaaccgccc tcacgccagc gtccagagcc
     1801 tgccatccga acagacaagg ttcgcaatca ccttctgcca taatgacttt cttcccgtcc
     1861 gggcgctcag tgctgattcg cttgacctgc aacaactcac catcgcgttt gtacggaagc
     1921 accagagcat ccagttcccg ctctccattc cacaccttgc cgctgacaac ctcgtagcgc
     1981 tttacgattt ctggcgatat gccacgcgat tgcaggtact caagatggga ttctgttctg
     2041 gtaacgtagc gggcgatttt cttgcggtca ggtctggaga attttttctc acgtttggca
     2101 tcgaaatggt gatcgtcatc cttgataccg agaaatgctt tcgcttcctg catagcctga
     2161 tgcaggttaa ttccacgaca tgccatccac aaatcaagca tgtcaccgcc gtctccctca
     2221 gcgaaatcag cccatttttt cttgccgcta atgttgacct taaggctgtt tcccttgtca
     2281 ccgttgacgt taccggcaac ccactcatgc ccctctttct tgccgtttgg caacaggtgc
     2341 ggagccaccc tgtcaacctg cgcccaaagc aggtcgctca gttcacttgg agtcattacg
     2401 ctgacctcag atcgagacgg ttaaaccaga actcaacgaa tgcagaacta agccagccat
     2461 ggttatagcc agcgataagt aacgatttga ttctggattt catggttcac ctgtcgaaaa
     2521 acacgtagcc agttttcgat acggtgattg cggatgatgg tttggattgt ggttgaatag
     2581 tttctggctt ctcgtcgttc cagcgttgac cgttcaggta gctcgatggt aacaacctgt
     2641 cgaacccgaa ctgcttaccg agcctgcacg caatgtcttc tgccagcatc gtggcaaact
     2701 cgcttgccgt ccccctggta gttttacgcc attccctgaa ctgtgttctg aatgctgaag
     2761 ctgcgttttt cttcccggct ttccgcatgc ctgcacacca gaatatttcc tcgaatgcct
     2821 tgtcggtttc ttcgtgacgg tcaggagttt tttcacactc tgttcggaca tgttcgaaca
     2881 taatgttttt aggttcattg actggttcaa aagagtgata ggttctgggg gcagctcctg
     2941 ccccacctga ttctggttgg atttgttgtg cattatccag cgtcagataa aaaacgtttg
     3001 actggttaag ctctcctttt cttctgaatt cccttttcaa aagccccatc tcttccagtg
     3061 ccctaatgtg actttttact gtcgatctgc tcacctcaca ctggtcagcg acatgttgat
     3121 atgaaggcca gcattcgcca ttatcattgg cgttatcggc aagtttaatc agaaccagtt
     3181 ttctcagtgg gttgccaacc tttatattca tggccttagc cataagattc atgctcattt
     3241 tgacttctcc gaagttttgt acctgttaag tatttctctc agtggcacag ctattgctgg
     3301 attaaccccc tgataaaact ggtcacgtag cacatctttt cggtgattaa cgcgtttatt
     3361 ttcctgcgtt tttcgcatat aattacctcg ttggatgttg ttaaaattcc atttgtattt
     3421 gatcagaacg ctcggtcttg cacaccgggc gttttttatt ggtgagtcca tcaagcgcat
     3481 acttaaaagc cctgctaatc ggactgatgt ctgatgccat tccgaaagca cacaagaccg
     3541 aagcaataaa tctccagtcc gttctgctta tcttcgattc atgacagcca atcatctttg
     3601 ccagaccgcg ctgggtaagc gttgacaggt tgatgagtaa atcagtttca gcgcgatcaa
     3661 tttctcgctg tgatagtttg ctgtaacttg tttgttccat ttcttaagat ttccaatagt
     3721 gaatagctag ttgaaaggta tgcgtggaaa cgcatatggc cttagttggt cagatatatt
     3781 gggactcgct ttgtcagcga cgtaggacga atgtccattg tgaaaagagc ggtgttactt
     3841 atgcagttgt ttttttgtta cttgggaagg gctttatttc ttccgcataa acgcttccat
     3901 cagcgtttat agttaaaaaa atctttcggc ctgcatgaat ggccttgtta atcgcgcttt
     3961 gatatacgcc gagatcttta gccgtcttgg tttgcccaaa gcgtattgca taatctttca
     4021 gggttatgcg ttgttccata caacctcctt agtacaggca atcattatca ccgctagagg
     4081 tataatagtc aacacgcacg gtgttagata tttatccctt gcggtgatag atttaatgca
     4141 tgagcgcaaa aaagaaaccg ttaacacaag agcagcttga ggacgcacgt cgccttaaag
     4201 ctatttatga aaaaaagaaa aatgagcttg gcttatccca agaatctgtc gcagacaaga
     4261 tggggatggg gcagtcaggt gttggtgctt tatttaatgg catcaatgca ttaaatgctt
     4321 ataacgcagc attgcttgca aaaattctca acgttagcgt tgaagaattt agcccttcaa
     4381 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg
     4441 agtatgagta ccctgttttt tctcatgttc aggccgggat gttctcgcct gagcttagaa
     4501 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg
     4561 cattttggct tgaggttgaa ggtaactcaa tgaccgcacc aacaggttcc aaacccagtt
     4621 ttcctgacgg gatgttaatt cttgttgacc ctgagcaggc tgttgagcca ggtgatttct
     4681 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc
     4741 aggtgttttt acagccacta aacccacaat acccaatgat cccatgcaat gagagttgtt
     4801 ccgttgtggg gaaagttatc gccagtcaat ggcctgaaga gacgtttggg tgatgtgata
     4861 gagatcatta ttcaattcga ttgagaaaaa tcatcataag gtttatatcc atttattaga
     4921 ttttatgtgt ataaatagtt aaaaatgcac gatgtagaga tatttgtgct taacatcaat
     4981 taatcattgc taaatcgtgc ttattgttca gaaatcgccc aagtcatatt gaaaatctgt
     5041 tggcgaacac ctatataagg agtatagtta gtgacccaaa acgtattgcc aatcaacaag
     5101 agcttgcatg accgagctgt tgatgaattt aatcgtctgc atggaacaat gattggcgag
     5161 attagtgcaa tgctgaaaac agctaaagta gcgccattgg tggatcttcg taagaaagat
     5221 cctacctttt cgaacgttgt cgcagaatta cggacattca gagacgtatg taatgctctg
     5281 ctcccatgct ttcgtgtaga taagaccagc gaaatcgctg tcatcgataa gttacttata
     5341 ctggctagtg acctggctca agccatcgat gctgacgatc ctgacgcatt gtgcgcagca
     5401 atagcagccc ttgatgttga gccttacatt taaacgaggg gaaaaaaaat gactaaagcg
     5461 tttgactata ctgccgttag taagcttttg gctgaaatgc gcggatgtgt agagcgcgtt
     5521 caaaatctac gccgcgactt tgaagctcac atcaatcatt cacaaaaagc ggcttgatac
     5581 ttaagttaga tgacagaacc cggcctcagc gccgggtttt ctttgcctca cgttcgccca
     5641 cctaaaaaca cataaccaat tgtatttatt tgaaaataaa tagatacaac tcactaaaca
     5701 tcgcaattct gatctctcgc cctccaaagc aataccccaa ttcaaaaaat aaattcatat
     5761 aaaaaacata cagataacca tctgcggtga tgaattatct ctagcggtgt tgacataaat
     5821 accactggcg gtgatactaa acacatcagc aggacgcact actcaccagg gcggtgaata
     5881 tacaacgatt cgaatatgaa tctacggcgc tgacaaagcg caataaccaa agtgaacttt
     5941 ggggtgaatg cagaagctaa ccttctcggc ggaggcgctt tgcaatgatt acgcgaccgg
     6001 agttagtcgc ccggctgtat tcaccaccaa agttcatcag gaggtctata tgacacgcag
     6061 aactcagttc aaaggcaatt cacgttctcg tcgtcgtgag cgtttaaagg caaaggcatt
     6121 agctaacggc gtgctggccc gcgaagaagc aataagttca gaagtattac accgccctac
     6181 tctaagcaga gcgcagattc aggctaaagg tactcacgaa acgcctgagc gcatagaaga
     6241 cgctaagcca attaagttca tggcacagga cgtgatctgg caacagaaag aatacagacg
     6301 caatctggag cgagcggcca ttgtgtacgc gaatgagttt ggacataagc aaccagaaac
     6361 tggtgtatgt cttccaaacg tagccattta cgcggcaggc taccggaaat caaaacaact
     6421 gacggcgagg tgacttgtgt tggtcgccag aaaatgaaat taggcagcaa accacttatt
     6481 tgaggtgata tatggaagaa caagcaaaca agattctcgt agaactactg caaaaagcca
     6541 gcaatggaat agacgcggct gtttcattta gccaggcaca gattcctgat gtcatccatc
     6601 agcttttgat gtggcacgcc gtatcatcag ctggaactca ggctatctgt gtattggtga
     6661 ttatagcgtg tgtttatctg atgatttttg catggaataa aggagatgat gcagatattg
     6721 ttcttttatc actacttgtc acatcaggaa tagcgattac ttctattgtt gttttcttca
     6781 atcatttcga ctggctgaaa atttggcttg ctccaaaact ttaccttatc gaatatgcag
     6841 catcattggt taagtaattt caggccgcat agtcggcctt tatttttggc ataaacaaga
     6901 gaataaacac tgcactgaat tatttgaggt gagatatgac aaaatcatgg agcgtacctt
     6961 ttcctgaatc agaaactgaa catgatggaa tgcctgtttt ctggagattc caggcgacag
     7021 ttgaagaaga tgggataaaa atattcgcac ttcaatatat agcttttcat cagacagagc
     7081 attatgcatg gttggttcct gcgcattgga ttgttaattt taaaccagca ccaaatcagt
     7141 ggttacagga atggaaacaa aggagaaata gatatgcaat taagaaagta gcaaaaaatg
     7201 cagaaagatc ttttgcattc ccaacgaaga aacttgccat tgagagttta ttgcgccgga
     7261 agaaatacca tttaatgaga atcaaacaag atttggctgt tgtatcaact cttgttgatg
     7321 ggatgaagaa tattgataca tcaacaccag atattgaata taactttggg cacaaccaag
     7381 aaacagaaaa ttgggtattt tattagtacg agtaagcact gtgtattcat tccaacgagt
     7441 gaatacacgg agcaatgtcg ctcgtaacta aacaggagcc gacttgttct gattattgga
     7501 aatcttcttt gccctctaat gtgagggcga tttttttctg tgaggatatg aacagatgtc
     7561 aaacatcaaa aaatacatca ttgattacga ctggaaaaca tcaatagaaa ttgaaatcga
     7621 ccatgactta atgacagagg aaaaactt
//
LOCUS       DAZFZC010000164         7515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFZC010000164 DAZFZC010000000
VERSION     DAZFZC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.166"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            292..807
                     /gene="ehxC"
                     /locus_tag="ACHKXP_004702"
                     /gene_synonym="hlyC"
     CDS             292..807
                     /gene="ehxC"
                     /locus_tag="ACHKXP_004702"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO7649545.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            809..3805
                     /gene="ehxA"
                     /locus_tag="ACHKXP_004703"
     CDS             809..3805
                     /gene="ehxA"
                     /locus_tag="ACHKXP_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO7649546.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3855..5975
                     /gene="ehxB"
                     /locus_tag="ACHKXP_004704"
                     /gene_synonym="hlyB"
     CDS             3855..5975
                     /gene="ehxB"
                     /locus_tag="ACHKXP_004704"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO7649547.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5979..7418
                     /gene="ehxD"
                     /locus_tag="ACHKXP_004705"
     CDS             5979..7418
                     /gene="ehxD"
                     /locus_tag="ACHKXP_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO7649548.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDIWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         2434 a         1151 c         1652 g         2278 t
ORIGIN      
        1 tgtattctgt ttacaatctc tttaataagg catttgtcac gtggctattc atatgaaaat
       61 catacgttat cttacaaatc aatcatctga gtgttataat ataacttagc tgtgatatgt
      121 gtaagaatgt ttaggcaata tattattatc gctaatgttg ttttggttgg gagagattct
      181 ccttgcggta gctttttgtt tttagatgct tcttgcttaa aaagatataa tttctgttct
      241 tttatatagg attctttaca tttactgagt tttcttattt atttaaacat tatgaaatct
      301 aatgcttttg acgtcatggg gaaggttgca tggctatggg cctgttctcc tctgcataaa
      361 aaatggccat tatctgtttt tgctattaat gtcatacctg ctattcagac aaatcaattt
      421 gctctcttaa taaaggatga gcttcctgta gctttttgta gctgggccag tttagatctg
      481 gaatgtgagg taaaatatat aaatgatgta acatctttgt atgcaaaaga ctggatgtcg
      541 ggagaaagaa agtggtttat tgactggata gctccatttg gacacaacat ggagttatat
      601 aaatacatga ggaaaaagta tccttatgag ttattcagag caataaggtt agatgagagc
      661 agtaaaacag gaaaaatagc tgaatttcat ggagggggga tagataaaaa actggcatca
      721 aaaatattca ggcaatacca tcatgaactt atgtctgaag taaaaaacag acaagatttt
      781 aattttaata ttgagaaaga aaactaatat gacagtaaat aaaataaaga acattttcaa
      841 taatgcgaca ttgactacaa aatcagcatt taatacagca tcatcaagcg tacgttccgc
      901 tggcaaaaaa ctcatattat taatacctga taattatgaa gctcagggcg tgggtattaa
      961 tgagttggtc aaagctgctg atgagcttgg aatagaaata caccgtactg aacgagatga
     1021 tacagcgatt gcaaaccagt tttttggtgc agcagaaaaa gttgtaggat taactgaacg
     1081 tggtgttgca atattcgcac cacaacttga caaacttctg cagaagtatc agaaagttgg
     1141 gagtaaaata ggaagaaccg ctgaaaatgt aggtaataat ctgggaaaag ccggaacagt
     1201 tctctcagca ctacagaatt ttacggggat tgctttatca ggcatggctc ttgatgaatt
     1261 gctgagaaaa caacgggaag gagaggatat aagtcagaat gatattgcca aaagtagtat
     1321 tgaacttatt aatcagcttg tagatacagt atcaagtata aacagtaccg ttgattcatt
     1381 ttctgagcag cttaaccagc ttggctcatt tttatccagt aaacctcgct taagttctgt
     1441 tggtgggaaa ttacaaaatt taccagacct gggctccctg ggggatgggc tggatgttgt
     1501 ctccggaatt ctttctgctg tatcagcaag ctttattctg ggaaacagtg acgcacatac
     1561 aggaacaaaa gctgcagcgg gtatcgaact gacaactcag gttcttggaa atgttggtaa
     1621 agctgtttcg caatatattc tggctcagag aatggcacag gggttatcga caacagctgc
     1681 aagtgcgggt ctgatcacat cggctgttat gctggctatc agtcctcttt ctttcctggc
     1741 tgctgcagat aaatttgagc gagctaagca gcttgaatca tattctgaac gatttaaaaa
     1801 attgaattat gaaggggatg ctttactcgc agcctttcat aaagaaaccg gagctataga
     1861 tgcagccctg acaacaataa atactgtcct gagttctgta tctgcgggag ttagtgcagc
     1921 ctccagtgca tccctcatag gggccccgat aagcatgctg gtgagtgcat taaccggtac
     1981 gatatctggc attctggaag catcaaaaca ggctatgttt gagcacgttg cagagaaatt
     2041 cgctgctcgg atcaatgaat gggaaaagga gcatggcaaa aattattttg agaatggcta
     2101 tgacgcaaga catgctgcgt ttttagaaga ctctctgtct ttgcttgctg atttttctcg
     2161 tcagcatgca gtagaaagag cagtcgcaat aacccagcaa cattgggatg agaagatcgg
     2221 tgaacttgca ggcataaccc gtaatgctga tcgcagtcag agtggtaagg catatattaa
     2281 ttatctggaa aatggagggc ttttagaggc tcaaccgaaa gagtttacac aacaagtgtt
     2341 tgatcctcaa aaagggacca tagacctttc aacaggtaat gtatcaagtg ttttgacatt
     2401 tataacacca acatttaccc caggagaaga agttagagaa agaaaacaga gtggtaaata
     2461 tgaatatatg acatctctta ttgtaaatgg taaggataca tggtctgtaa aaggcataaa
     2521 aaatcataaa ggtgtatatg attattcaaa attgattcag tttgttgaaa agaataacaa
     2581 acactatcag gcgagaatga tttctgaact cggagataaa gacgatgtgg tttattctgg
     2641 agcaggctca tcagaagtat ttgctggtga aggtcatgat accgtatctt ataataagac
     2701 ggatgttggt aaactaacaa ttgatgcaac aggagcatca aaacctggtg agtatatagt
     2761 ttcaaaaaat atgtatggtg acgtgaaggt attgcaggaa gtcgttaagg aacaggaggt
     2821 gtcagtaggg aagcgaacag agaaaataca atatcgtgat tttgaattca gaaccggtgg
     2881 aattccttat gatgtaatag ataatcttca ttctgttgaa gagctcattg gcggaaaaca
     2941 tgatgatgaa ttcaaaggcg gtaagtttaa tgatatattc catggcgcag atgggaacga
     3001 ttatatcgaa ggtaattatg gtaatgatcg actatacggc gatgatgggg atgattatat
     3061 atccggagga cagggagacg accagttatt tggtggtagt ggaaacgata aattgagtgg
     3121 aggggatggt aataattatc tgacaggagg aagcggtaat gatgagcttc aggcacacgg
     3181 agcttataat attctgtcag gtggtactgg tgatgataaa ctttatggtg gtggtggtat
     3241 tgatcttctg gatggagggg aaggtaatga ctatctgaat ggtggttttg gtaatgatat
     3301 ttatgtttat aggcaaaact atggtcatca tacaattgca gatgaaggag gtaaaggaga
     3361 tcgtttgcac ttatctgata ttagctttga tgatatcgca tttaagagag ttggaaatga
     3421 tcttatcatg aataaagcca ttaatggtgt actttcattt aatgagtcaa atgatgtcaa
     3481 tgggataaca tttaaaaact ggtttgcgaa agatgcctca ggagcagata atcatcttgt
     3541 tgaggttata acagataaag atggtcgaga gataaaagtt gataaaatac ctcataataa
     3601 taatgaacgg tcaggttata taaaagccag taatatagca tctgaaaaaa acatggttaa
     3661 tatcaccagt gttgccaatg atattaataa gattatttct tcagtttcag ggttcgattc
     3721 aggtgatgaa cgattagcat ctttatataa tttatcctta catcaaaaca acacacactc
     3781 aacaacttta acgacaactg tctgagacta aaagaggagg tgatggccat tccaggagga
     3841 atggtcatcg ttatatgatg agtaaatgta gttctcataa tagtctgtat gcactgatat
     3901 tgcttgcaca atatcataat ataactgtca atgctgaaac tataaggcat cagtataata
     3961 cccacacaca agattttggg gtgactgaat ggttactggc agcgaaatcc attggcttaa
     4021 aagcaaaata tgtagaaaaa catttttcca gattgtcaat aatttcttta cctgcgttga
     4081 tatggcggga tgacggtaag cattatatat tgtctcgtgt tactaaagat tcatcacgct
     4141 atcttgttta tgatccagaa caacatcagt cactaacttt tagtcgggat gagtttgaaa
     4201 aactgtatca gggaaaagtc attctggtta cgtcaagagc aacagtagtc ggagagttag
     4261 ctaaatttga tttttcttgg tttatcccct ctgttgtgaa atacaggagg attttacttg
     4321 aggtgttaac tgtttctgct tttattcagt ttcttgcgtt aataacacct cttttttttc
     4381 aggttgtaat ggataaggtt ttagttcacc gggggttttc aacgttaaat attatcacaa
     4441 tagcatttat tatagtgata ctttttgaag tgatattaac cggagccaga acttatattt
     4501 tctctcatac tacaagtcgt attgacgtcg aactgagcgc taagttattc agacatttgc
     4561 ttgcattgcc tgtttcatat tttgaaaatc gcagggtcgg agagaccgtt gccagagtaa
     4621 gggaactgga gcaaattcgt aattttttaa ccggacaagc gttgacatca gttcttgatc
     4681 tatttttttc tgtaatattt ttttgtgtca tgtggtatta cagccctcaa ttaacactgg
     4741 ttatattatt gtcactacct tgttatgtta tatggtcatt gtttatatca cccttattac
     4801 gtcgacgtct tgatgataag tttctcagga atgcagaaaa tcaagctttt cttgtcgaaa
     4861 cggtaacagc aataaataca attaaatcca tggcagtatc accacagatg attgctacat
     4921 gggataaaca actggccggg tatgttgctt ccagtttcag ggtaaatctg gttgcaatga
     4981 cagggcagca ggggatacag ctgatacaga aaagtgtaat ggtaattagt ctatggatgg
     5041 gagcacatct tgtcatatcg ggagagataa gtatcgggca gttaatcgcg tttaatatgc
     5101 ttgcaggtca ggtcattgct cctgttatca gactggctca tctttggcag gattttcagc
     5161 aggttggtat ttctgttgag cgccttggtg atgtattaaa tacaccggta gagaaaaagt
     5221 caggcagaaa tatactgccg gaaattcagg gggatatcga atttaaaaat gtcagatttc
     5281 ggtattcttc tgacggtaat gttattttga ataatattaa tttatacata tcaaaggggg
     5341 atgttatcgg tatagttggc cgttctggtt caggaaaaag tacattaaca aaactacttc
     5401 agcgctttta tataccagag accggacaga ttttaattga tgggcatgat ttatcacttg
     5461 cagatccaga atggttacga cgccagattg gtgttgtatt gcaggaaaat atactattaa
     5521 atcgtagtat tatcgataat attacattag cttctccggc tgtatctatg gaacaggcta
     5581 ttgaggcagc cagacttgca ggtgcccatg attttattac agaactaaaa gaagggtaca
     5641 atactattgt tggagaacag ggcgttggtc tttcaggagg gcagcgccaa cggatcgcaa
     5701 tagcccgggc tcttgttaca aatcctcgaa ttcttatttt tgatgaagca accagtgctc
     5761 ttgattatga gtcggaaaat ataataatga aaaatatgtc aagaatatgt aagaacagaa
     5821 ccgtaattat tattgcacac aggttgtcaa ctgtaaaaaa tgcaaacaga ataattgtta
     5881 tggataatgg ctttatttct gaagatggca cacataaaga gcttatctcc aaaaaagaca
     5941 gtttatatgc atatttatat cagttgcagg cataacgtat gcgcttttac atgaaaggtt
     6001 tatgggattt ggtttgccgt tataagacgg ttttttctga tatctggaaa attcgtcaca
     6061 cacttgatgc tcctgtgaga gaaaaagatg agtatgcatt tcttcctgct caccttgaac
     6121 tcattgaaac accagtatcc agacgttctc attttgttgt atggagtatt ttattatttg
     6181 taattatatc tcttctctta tctgttcttg ggaaagttga agtggtttca gtagcaaatg
     6241 gtaagtttac tcatagtgga agaagcaaag aaataaaacc gatagagaat gcgattgtag
     6301 aaaaaataat ggtaaaagat ggctcttttg taaaaaaaaa tgatccatta gttgaattga
     6361 cggtgcctgg tgttgaatct gatattttaa aatcagaagc atctttgttg tatgaaaaaa
     6421 cagaacaata tcgctacgca attctttctg aatcaataca acggaatgag cttcctgaaa
     6481 taaggataac agattttcct ggcggagaag ataacgcggg aggtgaacat tttcagaggg
     6541 ttagctcatt aataaaagag caattcatga catggcagaa cagaaaaaat caaaagcagc
     6601 tcacattaaa taaaaaaata gttgaacggg atgcagcgct tgctcgtgtt agcctgtatg
     6661 agcatcaggt atcacaggaa ggaagaaaac tcaatgattt taagtatttg ttgaataaaa
     6721 aagctgtttc tcaacattca gttatggagc aggaaaatag ctatattcag gcaaaaaatg
     6781 aacatgcagt ctggcttgca caggtttctc aacttgaaaa agaaatagaa cttgtgcggg
     6841 aagaactggc actggagacg aatatcttca gaagtgaaat tatcgagaag cacagaaaat
     6901 caacagataa cattgtgttg ctggagcatg agcttgaaaa aaacaggcag agaaaagcat
     6961 catcttttat taaagctcct gtgagtggta ctgttcagga gttaaatata catacagaag
     7021 gtggtgttgt cacaacagca gaaacgctga tgattattgt ccctgataat gatattctcg
     7081 aagtaacagc atctgtactt aacaaggata tcggttttat acaacctgga caggaggtcg
     7141 ttattaaagt agatgcatat ccatatacac gtcatggtta ccttacaggg aaagtaaaaa
     7201 atataactgc agattctgtt tctgttccgg atacagggct tgtatttaac gtgattatat
     7261 ctgttgatcg gaatgatata cagggagaaa gaaaaaaaat ccctgttaca gctggaatga
     7321 ctgttatggc agaaataaaa accggggtgc gtagtgttat cagctatctt cttagtccat
     7381 taaaggaaac tattaatgaa agtttacgtg aacgttagta tataaataac atattggctt
     7441 tttatatgat atagatttct gaaataatct cgtaaagatt atctatgtta tttaaacaag
     7501 ctatgataca gtttt
//
LOCUS       DAZFZC010000165         7499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFZC010000165 DAZFZC010000000
VERSION     DAZFZC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.167"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            132..1406
                     /locus_tag="ACHKXP_004706"
     CDS             132..1406
                     /locus_tag="ACHKXP_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7649549.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RNGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRYYQSKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRLELQKELVNTDVEGEIIIRALSDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPSEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIATPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPIND"
     gene            1399..3495
                     /gene="cvaB"
                     /locus_tag="ACHKXP_004707"
     CDS             1399..3495
                     /gene="cvaB"
                     /locus_tag="ACHKXP_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V export peptidase/ABC transporter CvaB"
                     /protein_id="HFO7649550.1"
                     /translation="MTNRNFRQIINLLDLRWQRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLIYLRRKFNLSARGATLAGINGIAEQLGMATRALSLELDELRVLKTPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGIRYISREEMSRYFTGVALEVWPGSEFQSETLQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDRGLLTLISA
                     ALMFFILLKAATSTLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKLGDIQSR
                     FDSLDTLRATFTTSVIGFIMDSIMVVGVCVMMLLYGGYLTWIVLCFTTIYIFIRLVTY
                     GNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAHWLNMKIDAINSGIKL
                     TRMDLLFGGINTFVTACDQIVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSERVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMGPISLETNGLSYRYDSQSAPI
                     FSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGRVLINGIDIRQIGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARASHIHDVIMNMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSESEHFVNVAIKNMNITRVII
                     AHRETTLRTVDRVISI"
     gene            complement(3665..3976)
                     /gene="cvaC"
                     /locus_tag="ACHKXP_004708"
     CDS             complement(3665..3976)
                     /gene="cvaC"
                     /locus_tag="ACHKXP_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V"
                     /protein_id="HFO7649551.1"
                     /translation="MRTLTLNELDSVSGGASGRDIAMAIGTLSGQFVAGGIGAAAGGV
                     AGGAIYDYASTHKPNPAMSPSGLGGTIKQKPEGIPSEAWNYAAGRLCNWSPNNLSDVC
                     L"
     gene            complement(3954..4190)
                     /gene="cvi"
                     /locus_tag="ACHKXP_004709"
     CDS             complement(3954..4190)
                     /gene="cvi"
                     /locus_tag="ACHKXP_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HFO7649552.1"
                     /translation="MDRKRTKLELLFAFIINATAIYIALAIYDCVFRGKDFLSMHTFC
                     FSALMSAICYFVGDNYYSISDKIKRRSYENSDSK"
     gene            4351..5128
                     /locus_tag="ACHKXP_004710"
                     /pseudo
     CDS             4351..5128
                     /locus_tag="ACHKXP_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            5130..5399
                     /locus_tag="ACHKXP_004711"
     CDS             5130..5399
                     /locus_tag="ACHKXP_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HFO7649553.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            5396..5662
                     /locus_tag="ACHKXP_004712"
     CDS             5396..5662
                     /locus_tag="ACHKXP_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7649554.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWNPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDERSWMQMSDRVRLLGRGW"
     gene            complement(5718..6170)
                     /locus_tag="ACHKXP_004713"
     CDS             complement(5718..6170)
                     /locus_tag="ACHKXP_004713"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO7649555.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISITLGD
                     SVTVGSGAIILSPYICSGAVIGAGSVVVKPIENKGIYAGNPARLLRIL"
     gene            6463..6723
                     /locus_tag="ACHKXP_004714"
     CDS             6463..6723
                     /locus_tag="ACHKXP_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649556.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGVTYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            6720..7310
                     /locus_tag="ACHKXP_004715"
     CDS             6720..7310
                     /locus_tag="ACHKXP_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649557.1"
                     /translation="MTQSRRPSPLQRRMLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDVGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVHLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(7372..7497)
                     /locus_tag="ACHKXP_004716"
                     /pseudo
     CDS             complement(7372..7497)
                     /locus_tag="ACHKXP_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
BASE COUNT         2122 a         1635 c         1737 g         2005 t
ORIGIN      
        1 tttatcctga taactctcct atgctgtatg tttatatgat tttccttgaa acatataatg
       61 caaattttcg atttattttc catcattaat ccagataaac aacaaactaa tagtatgcaa
      121 ggagacatta tttgtttcgc caggatgctt tagaaaacag aaaaatgaag tggcagggac
      181 gggcaatatt acttcccgga ataccactgt ggttaatcat gctgggaagc attgtgttta
      241 ttacggcatt tctgatgttc attattgttg gtacctatag ccgccgtgtt aatgtcagtg
      301 gtgaggtcac aacctggcca agagctgtca atatatattc aggtgtacag ggatttgttg
      361 tcaggcagtt tgttcatgaa gggcagttga taaaaaaagg ggatcctgtt tatctgattg
      421 acatcagtaa aagtacacgc aatggtattg tcactgataa tcatcgccgg gatatagaaa
      481 accagctggt tcgtgtggac aacattattt cccgtctgga agaaagtaaa aaaataacgc
      541 tagataccct ggaaaaacaa cgtctgcaat acacagatgc gttccgtcgc tcatcagaca
      601 ttatacagcg tgcagaggaa gggataaaaa taatgaaaaa taatatggag aattacagat
      661 actatcagtc aaaaggactg attaataaag atcaattaac taaccaagtt gcattatatt
      721 atcaacaaca aaacaacctt ctcagtctga gcggacaaaa tgaacaaaat gccctgcaga
      781 taaccactct ggagagtcag attcagactc aggcagcaga ttttgataat cgtatctatc
      841 agatggaact gcaacgactc gaattgcaga aagaactggt taacactgat gtggaaggcg
      901 aaatcattat ccgggcgttg tctgacggga aagttgactc cctgagtgtc actgtagggc
      961 aaatggtcaa taccggagac agccttctgc aggttattcc tgagaacatt gaaaactatt
     1021 atcttattct ctgggtcccg aatgatgctg ttccttatat ttcggctggt gacaaagtga
     1081 atattcgtta tgaagccttc ccctcagaaa aatttgggca gttctctgct acggttaaaa
     1141 ctatatccag gactcctgcg tcaacacagg aaatgttgac ctataaggga gcacctcaaa
     1201 atacgccggg tgcctctgtt ccctggtata aagtcattgc gacgcctgaa aagcagataa
     1261 tcaggtatga cgaaaaatac ctccctctgg aaaatggaat gaaagccgaa agtacactat
     1321 ttctggaaaa aaggcgtatt taccagtgga tgctttctcc tttctatgac atgaaacaca
     1381 gtgcaacagg accgatcaat gactaacagg aatttcagac aaattataaa tctgcttgat
     1441 ttgcgctggc aacgtcgtgt tccggttatt catcagacgg agaccgctga atgtggactg
     1501 gcctgcctag caatgatatg cggtcatttt ggtaagaata ttgacctgat atatcttcgc
     1561 cggaagttta atctctctgc ccgtggagca acccttgcag gaatcaatgg aatagcggag
     1621 caactgggga tggccacccg ggctctttca ctggagttgg atgaacttcg agtcctcaaa
     1681 acgccgtgta ttctccactg ggatttcagt cacttcgtcg ttctggtcag cgtaaagcgt
     1741 aaccgttatg tactgcatga tccggccagg ggcataagat atatcagccg ggaggaaatg
     1801 agccgatatt ttacaggcgt tgcacttgag gtctggcccg gaagtgaatt ccagtcggaa
     1861 accctgcaga cccgcataag tcttcgttca ctgattaaca gtatttacgg tattaaaaga
     1921 acgctggcga aaattttctg tctgtcagtt gtaattgaag caatcaatct gctaatgccg
     1981 gtggggacac agctggttat ggatcatgct attcctgcgg gggacagagg gctactgacg
     2041 ctaatttctg ctgctcttat gttttttata ttactcaaag ctgcaacgag tacgctgcgc
     2101 gcatggtctt cactggttat gagcacgctc atcaatgtac agtggcagtc ggggctgttc
     2161 gatcatcttc tcagactacc gctggcgttt tttgaacgcc gaaaattagg tgatatccag
     2221 tcacgttttg actcccttga cacattgagg gccacattta ccaccagtgt gatcgggttc
     2281 ataatggaca gcattatggt tgtcggtgtt tgtgtgatga tgctgttata cggaggatat
     2341 ctcacctgga tagttctctg ctttaccaca atttacattt ttattcgtct ggtgacatac
     2401 ggcaattacc gacagatatc agaagaatgt cttgtcaggg aggcccgtgc cgcctcctat
     2461 tttatggaaa cattatatgg tattgccacg gtaaaaatcc aggggatggt cggaattcgg
     2521 ggggcacact ggcttaatat gaaaatagat gcgataaatt cgggtattaa gctaaccagg
     2581 atggatttgc tcttcggagg aataaatacc tttgttaccg cctgtgatca gattgtaatt
     2641 ttatggctgg gagcaggcct tgtgatcgat aatcagatga caataggaat gtttgtagcg
     2701 tttagttctt ttcgtgggca gttttcggaa agagttgcct ctctgaccag ttttcttctt
     2761 cagctaagaa taatgagtct gcacaatgag cgcattgcag atattgcatt acatgaaaag
     2821 gaggaaaaga aacctgaaat tgaaatcgtt gctgatatgg ggccaatatc cctggaaacc
     2881 aatggtttaa gctatcgtta tgacagtcag tcagcaccga tattcagtgc tctgagttta
     2941 tctgtagctc cgggggaaag tgtggctata actggtgctt ccggtgcggg aaaaaccaca
     3001 ttaatgaaag tactatgtgg actatttgaa cctgatagcg ggagggtact gataaatggt
     3061 atagatatac gccaaattgg aataaataat tatcaccgga tgatagcctg tgttatgcag
     3121 gatgaccggc tattttcagg ctcaattcgt gaaaatatct gtggttttgc agaggaaatg
     3181 gatgaagagt ggatggtaga atgtgccaga gcaagtcata ttcatgatgt tataatgaat
     3241 atgccaatgg gatatgaaac attaataggt gaacttgggg aaggtctttc tggcggtcaa
     3301 aaacagcgta tatttattgc acgagcctta taccggaaac caggaatatt atttatggat
     3361 gaggcaacca gtgctcttga ttcagagagt gaacatttcg tgaatgttgc cataaaaaac
     3421 atgaatatca ccagggtaat tattgcacac agagaaacaa cgttgagaac tgttgataga
     3481 gttatttcta tttaaaccat agaggaatta caagcgtatg aggaatattt cttcctgtta
     3541 taattcctcg ttatgctcag atatctgttg gaggtggaat ggaagataga caatccacca
     3601 agaagaaata tcattctgtg tggattgtcc aataactgtt ctttcttata ttaaataata
     3661 ctatttataa acaaacatca ctaagattat ttggactcca attacacaat cttcccgcag
     3721 catagttcca tgcttctgaa ggtatccctt cgggtttttg cttaattgtt ccccctaaac
     3781 cggatggaga cattgcagga ttaggtttgt gagtggatgc atagtcatat attgcacctc
     3841 cagccacacc cccagcagct gctccaattc ctcctgcaac aaattgcccg gatagtgttc
     3901 ctatagccat cgcaatatca cgccctgaag caccaccaga aacagaatct aattcattta
     3961 gagtcagagt tctcatatga tctccttttt atcttatcgg atattgaata ataattatca
     4021 ccaacaaagt aacatattgc agacattaat gcagagaagc aaaatgtatg catggataaa
     4081 aagtcctttc ctctaaaaac acaatcatat atagctaatg caatatatat tgcggtggca
     4141 tttattataa atgcaaataa caactctaat tttgttcttt ttctatccat tactttttat
     4201 cccattactt tctatcccat taccacacaa acactaacga taatgattat cgttaacata
     4261 gtcaagagtg aagggtagga ggccctcaac cccctataag gggtccgctt ggaaaacgga
     4321 tttccccacg tcaagagaat tgatttgaac gagtggcttc gctacaaaca gctcccgttt
     4381 gtgtgtgaaa aacccctctc aacagatctc aactctgcac gatatctaca ggagattgct
     4441 ccaaaaggca gtatcgtgtc ttttaactac cgatataacc ccgtcatcga catgataatg
     4501 cgtctgagaa atgagaagaa attaggtgaa ctgcatttct tctcagcaga atttaataaa
     4561 aattctgcac tgacccgcca tcacctgacc tggcgagact ccgcacaaca gagcaaaagc
     4621 agcggtgctc tgggcgatct atcctgtcat ctgcttgatt tattttgttt tataggggaa
     4681 agcccggtag tggttcatgg gatcaaaacg gtaaagggga cacgtgggta caaaatcaga
     4741 tggtcaggtt gaagtggatg ataacggcta tgtaatgggc agttcagaaa agggagctta
     4801 tttcagagtt catgccagta aatcggaaac agatcataat ttgggactgc atatacagct
     4861 tgtatttgaa aatggtgaaa tcagatattc aacccaccat gaaaatcgcc tgctcctgat
     4921 tctatttaat gatacgaata cggaaacaat tggcttcgac gctctgaaac gcttgcctga
     4981 tccaccacgg gagcttccat tctggtctga ttcgtttatt catcttcatg acgactggtg
     5041 tgctcttatt aaatatggtc actcgtctcc taagctggcg gacctgtcat ccggccttca
     5101 tatacaggaa ataattgagg cattctgata tggataccat tcttttaacc gggctgtttg
     5161 ctgcattttt cacaacgttt gcgttcgctc cccagagcat aaaaactatc aggacacgca
     5221 ataccgaagg tatctccgtt gttatgtata tcatgttcct gacaggtgta atctcatgga
     5281 tagcgtatgg cattatgcga tctgattttg ccgtactgat cgccaatatt gtcacactgt
     5341 ttctggctgc accagtgctt gtaataacac ttatcaaccg caggaaaaaa cacgtatgaa
     5401 tattgcaatc atcggcgcgg gtcctgccgg catcatttct gcccgtaatg cgattaaagc
     5461 aggtcactcc gtggttctgt ttgagaagaa tacccgaatt ggggggatct ggaacccctg
     5521 gagtggtggc gcttaccgta atgcctgtat gcagaactct cgttatacat ttcattatac
     5581 tggctttccc cctggcgata tcgatgaacg aagttggatg caaatgtcag atcgtgtgcg
     5641 cctgttaggg cgcggctggt aagagagcct tataggcgca tttgaaaaac ctccggctat
     5701 gccggaggat atttatttca taatattctg agcagtcttg ccgggtttcc tgcataaatg
     5761 cctttgttct caatcggttt taccacaact gaacccgcac caataacagc tccgctgcat
     5821 atatacggag acagaataat ggctccactg ccaacagtaa ccgaatcccc cagggtaatg
     5881 ctgatccagt tatccggtga cggatctggt gctcctgaac gaaacagatc gttggcgaac
     5941 gtcacgttat gaccaatgaa gcagttctcc cccagtgtta cgttctcaca aataaacgtg
     6001 tgcgactgta tccggcttcc actaccaatg acacagcctt tctgtatctc aacgaagggg
     6061 ccgacaaaca cgttgtccct gagttcacat tcataaatgt tgaccggttt gacaatacgg
     6121 acattggtcc cataaatcac atcccggaca gcacattcca taaatttcat actcacacgt
     6181 cctttaaata cagatgacac ttgccggaat tatacagccc agattcactt gctatgtttc
     6241 agaatagctt ataagccgca cttttaccga tactcagcaa caaatcaggt ctggatccac
     6301 ttcagccgga aaccggatct gcgcggcggg gaacgggcat acaatatgat aattaagcaa
     6361 cttatataaa accatatctg atattattaa taataaataa cacacaaatc atgcgggata
     6421 tgatttttat ttgtcagaac attatcagat ggagcgtgca ggatggatga agaaagggtg
     6481 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
     6541 ctggacagcc agggcgtaac ttatctctgc gagtcagcca aagccggagc tgtgctgatc
     6601 ttctggcatg aactggccat aaatggctat gcctcaatga atgccataaa gagacaggag
     6661 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
     6721 tgactcagag ccgccgaccg tcgccactgc agcgaagaat gctgattgtg ctggctgccc
     6781 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcgaa
     6841 gcggggaagc cccagtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg
     6901 ccggctggct gcgtaccctg cgcgctccga acctgcagct ggcagtggag ctgacggacg
     6961 tcggccgggc cgtggcacag cctctgctgc tggcagaaca ggaccgcctc cgggcagaac
     7021 agcgtgcggc agaagtcgtc gtactgccgc tggtcccggc agcaggtctg cccgcagatg
     7081 gcacttctgc cactgatctg gcggtgcagc tgaacggcat aacgtatcag gcctgccgtg
     7141 gggattttgt ggtgcatctc gacggcagca cctgtctgca gttatggaat aaagaaggca
     7201 gggtggtacg ccgggagggc gatccgctgg aagtggcgca gtggctgcag gcctgtcacg
     7261 atgcaggaat ggaggttcgc gtacagataa atgaaagtgc cgctccgtaa gaacacgcct
     7321 gtgcacggtt tacacatcat cccatggtgg cagccggtta actttcctgc gcccccagta
     7381 gataaaccac attgcacatc cccaggaaat gccccataag ccccccgtca gcaccgtgac
     7441 ctgccagaac ggcataaagg gtaatggcgg aagccggata cccagccgcc agagaatac
//
LOCUS       DAZFZC010000166         7494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFZC010000166 DAZFZC010000000
VERSION     DAZFZC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.168"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..910)
                     /locus_tag="ACHKXP_004717"
     CDS             complement(<1..910)
                     /locus_tag="ACHKXP_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7649558.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAVCKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQK"
     gene            complement(1408..1677)
                     /locus_tag="ACHKXP_004718"
     CDS             complement(1408..1677)
                     /locus_tag="ACHKXP_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO7649559.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1689..2648)
                     /locus_tag="ACHKXP_004719"
     CDS             complement(1689..2648)
                     /locus_tag="ACHKXP_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO7649560.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(2739..2815)
                     /locus_tag="ACHKXP_004720"
     tRNA            complement(2739..2815)
                     /locus_tag="ACHKXP_004720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2779..2781),aa:Arg,seq:tct)
     gene            complement(2829..2905)
                     /locus_tag="ACHKXP_004721"
     tRNA            complement(2829..2905)
                     /locus_tag="ACHKXP_004721"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2869..2871),aa:Arg,seq:tcg)
     gene            complement(2915..2990)
                     /locus_tag="ACHKXP_004722"
     tRNA            complement(2915..2990)
                     /locus_tag="ACHKXP_004722"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2955..2957),aa:Ile,seq:cat)
     gene            complement(3432..3866)
                     /locus_tag="ACHKXP_004723"
     CDS             complement(3432..3866)
                     /locus_tag="ACHKXP_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7649561.1"
                     /translation="MRDIRQVLECWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGGTFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(3859..4053)
                     /locus_tag="ACHKXP_004724"
     CDS             complement(3859..4053)
                     /locus_tag="ACHKXP_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO7649562.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(4050..4655)
                     /locus_tag="ACHKXP_004725"
     CDS             complement(4050..4655)
                     /locus_tag="ACHKXP_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7649563.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(4655..5377)
                     /locus_tag="ACHKXP_004726"
     CDS             complement(4655..5377)
                     /locus_tag="ACHKXP_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO7649564.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMERGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5452..6186)
                     /locus_tag="ACHKXP_004727"
     CDS             complement(5452..6186)
                     /locus_tag="ACHKXP_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO7649565.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKLIQSGYSGICHDYGYKFSYIIGRARGVLINHTRDIDIY
                     EPDGPTNLLAWERLKNKELPPSLHRY"
     gene            complement(6461..6643)
                     /locus_tag="ACHKXP_004728"
     CDS             complement(6461..6643)
                     /locus_tag="ACHKXP_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7649566.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(6640..7167)
                     /locus_tag="ACHKXP_004729"
     CDS             complement(6640..7167)
                     /locus_tag="ACHKXP_004729"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7649567.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(7164..>7494)
                     /locus_tag="ACHKXP_004730"
     CDS             complement(7164..>7494)
                     /locus_tag="ACHKXP_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7649568.1"
                     /translation="RSKAQNDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTK
                     KLEQRSVPGIDGGVVMLGVRTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWS
                     QRKGRAA"
BASE COUNT         1774 a         1993 c         1683 g         2044 t
ORIGIN      
        1 tcttctgctt ccagaaatac gtcgtatctc cacatatcca gggaacaccg ccagcagagc
       61 caccgacgca ctgacctgcc atatccgcca ggtctgcacg gaatttatca accagcgcac
      121 caaactgtgc tgcgtgattt accggcgtac cgccaaaatc aaattccccc tgcatccaca
      181 ccacggcaaa cagcacattt ttcggattct tcttcagtgc tgcttttgtt cgaccgataa
      241 ggtccttata cagcggcttg tccacacccc agcgggttga attctccgag gcaccactcg
      301 cgtcactgta tgtgccatca gctccggtgg tgaacgctga accaccacga cagcacggaa
      361 ccagcagaat gcccgcattc gccggtataa acggcagcaa ttttttggcg atatgcagcc
      421 cctgccccac ggttccgtac tgcccctttg acaggtccgc tttcggatgg ttaaggcggc
      481 tcatgtcctg cacatcatgc agacaatggt ccgccggaat gatgtcgtta tatttgcata
      541 ctgcaccgcc cggtgtcacc gtactgcgac gcgctaactg tttaatacgt ggatcagggc
      601 tgtcgaatgt atccggcaat ggcagtccct caccgtatga cataccattg gactgaccag
      661 caagcgcgat cacatagtaa tattctggcg caacagaagg cgctgaggtc gtcggacggt
      721 tgcctggctc ctctggtgat gagatgctcc cctcactcac aactggctgg atgaactccg
      781 caccataacc agctgtcgaa atcagcgcac taccataagg ctgccaccct tccttcagtt
      841 tttgagttat tcgtttcgca aggtctgacg gcgacgccgc cctgacaaca tcatagtgtt
      901 taaatgccat gaatcctccc ggccgggata atattgtgag taaaataagg agcgggctga
      961 agtccggaag ttacaggaca atggcagaag ggagactaca gcccgcaatt cgaaaaaggt
     1021 cgcgcagttg cgcagagtga ttactatagg gtattattcg ccagctgaaa tattacttca
     1081 cgtttcattg tttattcctt gccgcccgcg tctcccagcg cgggcttttt tgtccataag
     1141 aaagcccctc cggagagggg ctggagagtg gcgctatgtg ccattgcatg gtgccgggtg
     1201 cctcccggtg agttcggcct ggtaccaccg aacccgtgtc gataatgaat cacaagcaag
     1261 gatttttcac cagtcgcccc tccacacagg gggattcacc atgcgaaatt tttttaacaa
     1321 atgctcagtc tgacaggcaa ctgtcaactg actgaattgt gacacagatt acacttgtta
     1381 cccacatacc acgaatcagg ttatgcctca gtcattatta aactgcactt cagcaaatcc
     1441 ggagcctgat tcacaggtac tggatttgat tgtgacagtc attcctgtca actgagcact
     1501 ttgcagtaac ggttgcagat tccagcgact ggtccagtat tctttcccgt caaccttcac
     1561 tgtaaatgtg tcatcctcat tatacttgga aaactcaatt ttacctttag cacaatccgc
     1621 cgccattgca ttaacagaag ctaatgcaaa taaaaccgcc ataaacatct tcttcatgct
     1681 taactccttt atttacccgt tgtatataaa aactgtgact ttctgttcag aaacgctgca
     1741 gctgtattac tttcccataa tgtattgttt atttttataa caggcctgtc gccagttatc
     1801 tgacattctg gttgactctc ttcattcacg gcgcgaacag aacgcgcccc ctgatgatgg
     1861 caattcagta taacggccac agtccccagt atcgctgata tattattaaa ggatattctc
     1921 cccactctga caccatcctc tccccgatac tccggaagca cattgctgat tcgcccccag
     1981 ttcagagtga ggtccacgtc tcccggcgtc atcgtataca caggagcagt ttcagacagt
     2041 gcctgacgaa attctctctg tatctgcctg aagcgtaagg cttctgctgt gacagtgaca
     2101 aaacgcagaa ctgctctgga tgcatctctg gtcattgtat taccactgaa ctccattaac
     2161 gccagatatg atgaaaccag tgagtgacga ctgatttgca ttccggaacg ttccagcgct
     2221 gcgacacgtt gcagagtggt ataactgctg tccgttgtca tggaaaccgt tgtcacaccg
     2281 ggcactgata tatgtgtaaa atctgaaaaa cggtagaaag tatttgttgc cgtattaacg
     2341 aacccggcca catataaatt attttgctca ataatcagac gaagatggtc aaaacgcgcc
     2401 tgatagacat caagccctcg tatatccaca gcaaaataac tgcccggtgg ggtgtggtta
     2461 ataacagaca ccgatgtggt cccctgagat atatgttcaa gaggggtcga tatctctgtc
     2521 cgtatactat ttaacgaaga gacataactt tgttgggtcg aaaagtctat cgtaaactcc
     2581 cgggaatagg ataccgaaga aaaacccagt aacaggcaca gtacccattt aaataatata
     2641 cacttcatat acaggtgttc cttttggctg aagtaatcag caccagaccc ggcgcagata
     2701 taaaaaaggc ccgcaaaagc gagcctggta aataaatatg gcgcgttgta ctggattcga
     2761 accagtgacc gattgctcag aaggcaattg ctctgtccgg ctgagctaac aacgcataat
     2821 gcagataatg gaccgccatc gaggactcga accccgcgca gccagcttcg aaggctggcg
     2881 ctctgtcccg atgagctaat ggcggtatgt gatatggtgg cccttgctgg atttgaacca
     2941 gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc gggcgcagga
     3001 taataacggt acgtaactaa tcctgcaata tcatccgttc tgactgacta cattctgaac
     3061 ttccctgacc gtctgctcaa aacgcccgct ctccagctca acgccaattg cacgacgccc
     3121 tagcgccatt gctgctttga cgctacggac ataaaaaagc cagccactgg gggaggctgg
     3181 caaactcgta gagcaaaatg ctgttacgca aacttcgtta cagggttatc ctgcaatact
     3241 taaaatatac aatatttaga aaactaatag tgctatatgc gatttttaag attttgttat
     3301 taattgtggt cgcaccttcc tttctgtgta ctttccgtat agctcacagg attctgggta
     3361 caaaaaaacc cgcgcatcgg cgggttaagc agcgtggcaa tgtaaccact cttatcatga
     3421 tatgcagatt tttacgatcg taaactattt tttcgctgat aaaatacaga ggttctccct
     3481 cccggcaatt cacgctcaac ataccgatcc atctcaagcc tcactcccag catcatcagc
     3541 atgccttcaa caatcccctc cgctttgtga aggcgtttac ctatacaggt gtcagagcac
     3601 ccatgtttcc gtgccagcgc catgaacgtc ccccccagca cgtaataatc aaccagcaag
     3661 tcatgcagat cgcgattgtt ccggtaaagg cgggctatac acccgcatat caccatcgca
     3721 tcatcgtcac agcactgcgg gcgtgatttt actttttcgg ggatcagtcc cttaaatccg
     3781 gcggcaatgg gtgaccaggt cacatcctca tggttatttg ccgcccatgc cccccagcac
     3841 tcaagaacct gccggatatc acgcatcagt atctttaccc catccgcgat gaaccataag
     3901 aacaccgttg acaatagcgt gtctttttcc ttctttatcg ccagtgtatt ttctgaccgt
     3961 gttgcgacta cagttcagta ttctggctac ctcggtctga ttttcatatg cctcaacgag
     4021 catgtcagga atggttttta ctgtgaacgt catgcggcct cacttctgct gtttcgcagg
     4081 tctttaagtt tctgctgata cttcgccttg atggccctgc actcttcgac agtccagcga
     4141 tggcggttat ggtttgattc gatttcgtct actgcttcct gcccgatgcg attaatcagt
     4201 tcgacgcgat acggaacgag atttccgctt ttgtgctggt tgcacaccac gcattgcttg
     4261 tgaatattgc gttcatcaaa tcggagttga ggtgccgcag cagttgtccg gtaatgtccg
     4321 gcatcccact gagcagacgt gagcgttccg cacgagatac atggtaagtc gcggtctctt
     4381 tctctgatga aggcgtttac ggcttgttgg gcttgtttaa tccagtaact gcggggcttt
     4441 aaggcgagtt ttcgaatctt aattttatct ttctgtttct gctcctctcg tcgtcgtttc
     4501 ttctctgctg ttttttccgc cttttcgcgt tctttatttc gtcgttcgag tgctaattta
     4561 gttccgtgtt ccgggctgca ccaccactga tttgagaatg ccgggtgaaa ccattcctta
     4621 cagattttgc atttccttcg cgctggttta gccattaagc agcctcccct gttactttaa
     4681 gcattccgtt atctagcagc tttcttgtca gccactgttg accacgcccg gtgatttttg
     4741 tggtgaacga tatctgtatt ccgtgatttg tattgaccgc tgtttctttc actgtgaaat
     4801 agccgcgctc catatattcc tgcattggca catttcgccg ggaacctgaa gcaataagga
     4861 ttttgtgatc gcgcatccac gcaaacagtt tgtttggacc aataccaaca acctttgcaa
     4921 agtttccaat caaaattccg ctggcctcgc caacgcgatc ggcaaactca actttaggtg
     4981 ctgcgagagc aagctgtttc tccagttcag ccttctggtc ttcaaggtcg gccgcaaggc
     5041 gcaatgcctc agaaaaggtt tgtggtattt tcgcggttgc cccttcgagt tctcgccagc
     5101 ggtcaacaag gcgagcggtg aattccggcg acaactgagc gacgacaata atgctgtctc
     5161 gcttaccttg ttcgccttcg aatatataca cgcttgtgaa tttgttaggg ctaattgttt
     5221 gtttattttc aactttttgc attgaaggaa gttgaatcac cccacgcttt gccagacgtt
     5281 ctattgatat tctgacatta ccgtgttggc ttcccaccaa ctcagcgatt tcaatgcttg
     5341 tcattttgat ggcattgcta tttatcagct cattcattgt catgtcctct cacattgaaa
     5401 attcagcaat aaaaaaccca gccgaagctg ggtttgttaa gttgtcaatt gtcagtagcg
     5461 atgcagtgaa ggcggcaact ctttgttctt aagcctttcc catgccagaa ggttcgtcgg
     5521 cccgtcaggc tcataaatat ctatatcccg cgtgtgatta attaaaacgc ccctcgccct
     5581 cccgatgata tacgagaact tatagccgta gtcgtggcat atgccggaat agccagactg
     5641 aatcagtttt aatgcgggat acaactcacg gaacaatgcc tgtgagcggt tggcataatc
     5701 ccacagccat acaaggctgt ctgtttcttt tgcggaaagc ccgttgagct tcttctcttg
     5761 tttgccagta tttttctcgc actggctgaa atagcagtct tccagttttt cgaacacatc
     5821 ccacgcctga tcagtttcga gcatttttgc gtgacgggct gcgcctcgtt ctgtccagag
     5881 tatgagggag cgggctttcg gggaaatttg taaccctctt aaagatggtt gcaaattttg
     5941 tgagttactt aaagtaaccc gcaaattttg tgagtagttt aaagctaccc gcaattcttt
     6001 aaggtcatta ccaacaactt tgaaaaagtg tttcccttca acgaagcgta ctttgttctc
     6061 atgatgattc tggcgaatac gcaccggctc agtgccgtaa agctgcgcca aaagttcggt
     6121 ggtaataaca ggaatctggt tatgggtgat cggggagaga gtttcaacag aaatttgagt
     6181 tgtcataatg acgccctctg gtggtttctt aataactcac caccgacgac gccaatcgtc
     6241 tggtggtgaa ctgtgcaggg ttggcgtaac cgggaaaccg accggcgcgg atctctccgc
     6301 ccccacacag cccaccataa ttcagatgtg cgcgtgcata cgacaataaa aaacacgctc
     6361 gcggcgtgta tctgtcgcgg tctctatcca ggacgccaat cccgacgcca gattttgctg
     6421 gcgcgtgagg aatatagccc cgaataaatc atcgcgtcaa tcaccttgtt ttcctcgcac
     6481 gatgtcttag ccaccggata tcccacaggt gagccgtgta attgaaggtt tttacgtcag
     6541 attcttttgg gattggcttg cgtttatttc tggagcgttt cgttggaagg tatttgcagt
     6601 tttcgcagat gatgtcggtg atacttcgtc gctgtcgcct catgccgccc tcctgacgcc
     6661 ctgcccgatc gccatcaatg ccgctttgga tacagtagta aacattcgtc gaggactgat
     6721 gaacggtcgc caaatcagca gcatggagcc tttgctgttt cccttcttct ccagccctgt
     6781 cgatggttcg ataaaattaa tccgtccatc agtgatgatg cgaacttcgt caacactctc
     6841 cagagccttg ctgaaccatc cgactgacat atcctctggc acaagcataa ctaccgtctg
     6901 tcgctgttgt atgcactgct cagcggcttt ttccacccac ggcctgatat tgctgtacgg
     6961 tgggttattc cagattgcac cgtggcttac ccactcagaa ttgagcgcgt cgtcggcctc
     7021 agttagccag tgagcgcaca gagcattttt gtcgctcgct gccgaatcca gccagaatcc
     7081 aaactcaata tccagtgcat caaaaagcca aagcggcgtt tgccagcagt ccttgtcgtg
     7141 tgctggcgta tttgatttga tagtcatgca gccctacctt ttcgttgtga ccattcatac
     7201 tctcgccggg agtcatcact ccaccgcacg ttgcgctctg agccgaacca gaacatgatt
     7261 tcgataagct cagtcatgct ggccttccgc attttgctgg tacgcacgcc aagcatgaca
     7321 acgccaccgt cgataccagg cacacttcgt tgctccagtt ttttggtctt aagccacagg
     7381 gcagtgaaca ggtctttcca gtcttccggc gccagccgtt gaccatgcca tagcacctga
     7441 cgcgaaacat cgttcagcat cggccacata cggtcattct gcgctttgct gcgc
//
LOCUS       DAZFZC010000167         7341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFZC010000167 DAZFZC010000000
VERSION     DAZFZC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.169"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            694..903
                     /gene="mchI"
                     /locus_tag="ACHKXP_004731"
     CDS             694..903
                     /gene="mchI"
                     /locus_tag="ACHKXP_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO7649569.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            931..1158
                     /gene="mchB"
                     /locus_tag="ACHKXP_004732"
     CDS             931..1158
                     /gene="mchB"
                     /locus_tag="ACHKXP_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO7649570.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            1430..2980
                     /locus_tag="ACHKXP_004733"
     CDS             1430..2980
                     /locus_tag="ACHKXP_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO7649571.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNNVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            3006..3458
                     /locus_tag="ACHKXP_004734"
     CDS             3006..3458
                     /locus_tag="ACHKXP_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO7649572.1"
                     /translation="MYYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            3611..4885
                     /locus_tag="ACHKXP_004735"
     CDS             3611..4885
                     /locus_tag="ACHKXP_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7649573.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISKTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            4878..6980
                     /gene="mchF"
                     /locus_tag="ACHKXP_004736"
     CDS             4878..6980
                     /gene="mchF"
                     /locus_tag="ACHKXP_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO7649574.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGTNLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIFRITN"
     gene            complement(7041..>7341)
                     /locus_tag="ACHKXP_004737"
     CDS             complement(7041..>7341)
                     /locus_tag="ACHKXP_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649575.1"
                     /translation="LALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADIAGSAIGGY
                     IGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSSSSSGS"
BASE COUNT         2085 a         1378 c         1724 g         2154 t
ORIGIN      
        1 tgtacctgat caggtatcag tttgcaaaaa tgtttgttat aggtcatgac cgcaaccagg
       61 acacataatt tcaccacaac cacaaatata atcggcatca tagtcatgtg ttactatacc
      121 ggaagccgac ttattatttg tgatacagtg tgtctcctgc tctacggagg attctacctc
      181 tataggaaaa ttatcgtgtt ttcgtcttga attgtcttgc ttgttgcctt tatcaaaggt
      241 aacaatgaat tcttcatttc tgtgattatt atcacaattc attcttatgc ctcctcacat
      301 tttccgatgt atgaccgtca gtgctattag tgtgcgttca agtctcaggt tatacatgag
      361 cgacaaccat gagatcctga ctaacaattg catcagtgac ataacgtaaa atacctcttg
      421 actttatatg gacaatatga cacttttttt gaaaagtaaa taacaatcat tctcttttgt
      481 ctttgtctgg gtgaggtcag gtaagatgga atgtgctaca aacagggttt ctgtaaatga
      541 cagtcggtca gcgctgtcat cagctttgct gttatctttg atcatgagcg ccactctact
      601 ggaatattcg ttatcgatgg cctgactctc ctgactgagg tgctaagatt tggtaacttt
      661 tcataatgaa aataaagcaa caggtaggga ttaatgagtt ataaaaaact gtcccaattg
      721 acggctatat tcagtttacc tattactatc ttattggttt cactttcatc ccttcggatt
      781 gttggcgaag ggaattctta tgttgacgtt tttctaagct ttataatatt tcttggtttt
      841 attgagctga ttcatgggat tcgaaggatt ctggtctggt caggctggaa aaacggaagt
      901 taaatatgat ggagtttata tgcagtttat atgcgagaaa taacagaatc acagttaaga
      961 tatatttccg gggcgggagg tgcgccagcg acttcagcta atgctgcagg tgctgcagct
     1021 attgttggag ctctcgccgg aatacctggt ggtccacttg gggttgtagt tggagccgta
     1081 tctgccggtt tgacaacagc aattggctcg accgtgggaa gtggtagtgc cagttcttct
     1141 gctggtggcg gtagctaatt atatttttat ttattttaca ggcacttttt cggtagtacc
     1201 tgtaaaatat atcctttttg tttaaaacag atagaagatc catttataag tgtgttttta
     1261 tgttcaacac tttactgatg aagatatgat ttacagtcgt tgataaatga ttttttaatt
     1321 tatgtgtaat taatagagtt ttactgtaat aaggaagaag ctgtcaggac acttacttgc
     1381 tcttttatga acactctttt atgaaaattg aaacggagaa tactgccaga tgagtcatca
     1441 gtgttcactt tctgaactga atgaaaacct ggtgcctttc actgccaggc agatcaaggc
     1501 ctcattaatc tggtgtgcag aggatgtcag aaatccagac gagctgcaaa atgcctgcag
     1561 ttatattatc gatcctggca gtacagcttc tgccaaagtg ttccatgcag agcgctatgg
     1621 tggcagtggt attcagcgta atggaggtgg tgcacgttgt gggtttgatg gtaactacca
     1681 ggttaaagga ataggaagta atccgttggt tggcgaaggt actgacgaac gtcattctaa
     1741 tggtgcactc ggcgctgttc atgcaatata tgaggctttg tggggagaag tactggctca
     1801 aatattacct tatagtgctg tgcgggttcg ggcggtttta cttacagatc tctatactga
     1861 aaaggcattt gagcgctccg gtaggaaatc acgaagagcc ctgttggtac gtgagcctgt
     1921 tgttcgtccg gcgcattttg aacgggcacc ttacttccaa gtaaaaccgg agtattccag
     1981 tctgttaatt cacgatgcct gtcgggttag atctgtgatc cacaagctgc caggatatct
     2041 acctgtacca ccggaagaaa ttgatgctga agcacgaact gatccccgga tttattgcat
     2101 tgaggggtta tgtgaactgg cacgtcgtga ggcctggcaa atggcatttt gtcgaacacg
     2161 tttcctgaga ttgacaactt ctccttctaa tattgcaatg gatggcagat taatggattt
     2221 taacggactc agttgtctgt ttccgggaga ttccccagct gattttgggt ataaactaag
     2281 attagctgaa ctggcaaaag aaccgatggt acttatgcaa gggctgtctg atctctgctt
     2341 gtatatcgga aaatatatgt ttgaccctga cttcactctt gcagcccgtt tgaaggttga
     2401 ggagatattt cagaaaactt ttcatgaagc atgttattac tgttatctag aactgttggg
     2461 tattcctgga gaatttataa cacaaaaaga gatacctgat atattgaaag aactggttaa
     2521 cagttttgtt gcattactca atcaatactg cgagaaatca catgcccaag atattgtcaa
     2581 tcaggatggt tcaccattgc aaaagttggt tgtgacgcta attcatcata ggcataatca
     2641 aaagcaggca ctgaatagta gcatcaagaa taatgtttat ttcaccgttg cacaacagtg
     2701 tttttcccag gccatccact ggctgacgca aggcagtacc agacgtcaga taaatgcctc
     2761 attactcctg aaagaaattg aacatcatac catgaaaagg ctgcaaccca gggaagagct
     2821 gaggaaagaa aatatgtgcg aaaaaattgc catcctgctg gataatcatg gcgatgatcc
     2881 ccttttttta caagaagcaa tttctgatat gaaaaatttt atgcttaagt tttccagaga
     2941 tgcatttgga tatcttgaac cgataagaaa cacagtgtaa aaaatatata agggaaatag
     3001 taatcatgta ttatataagg gaaaccatca gaggaaaaga tgaatggact gtttatgaac
     3061 agatcggttt tgcagtcagt tgtatgctct acaatcgtaa ttacagtctg tatccggtgt
     3121 taaccattca atactggact gaatatgcga tacagcataa tcagattaaa ttcctgtttg
     3181 attcacgagg ttttccactg gcgtatatta cctgggcata tcttgaggct gatacagaag
     3241 cgcgcctgct cagggatcca gaattcaggt tgcatccgtc tgaatggaat gaagatggaa
     3301 ggatttggat tctggatttc tgttgtaaac caggctttgg tcgaaaagtt attgactatc
     3361 tcatacagct tcagccatgg ggagaaggag aagtacgatg gttaagcagg cgaaagaaaa
     3421 ttgtgacata catccctgag cggctgcata aaacgtagta cctctgaaga tacagagata
     3481 attgtaaatt acggggtaaa tgcatcgctg atactatttt gacaggactc tgtattttct
     3541 ggctttgatt atagcatttc gcttaaccag tgaaggtatt acaggaaaag tgtgagtaag
     3601 aggagctatt ttgtttcgtc aggatgcttt agaaaacaga aaaatgaagt ggcagggacg
     3661 ggcaatatta cttcccggaa taccactatg gttaatcatg ctgggaagca tagtgtttat
     3721 tacggcattt ctgatgttca ttattgttgg tacctatagc cgccgtgtta atgtcagtgg
     3781 tgaggtcaca acctggccaa gagctgtcaa tatatattca ggtgtacagg gatttgttgt
     3841 caggcagttt gttcatgaag gacagttgat aaaaaaaggg gatcctgttt atctgattga
     3901 catcagtaaa agtacacgta gtggtattgt cactgataat catcgtcggg atatagaaaa
     3961 ccagctggtt cgtgtggaca acattatttc ccgtctggaa gaaagtaaaa aaataacgct
     4021 agataccctg gaaaaacaac gcctgcaata cacagatgcg tttcgtcgct catcagatat
     4081 tatacagcgt gcagaggaag ggataaaaat aatgaaaaac aatatggaga attacagaaa
     4141 ctatcaggca aaagggctga ttaataaaga tcagttaact aaccaggtgg cattatatta
     4201 tcagcaacaa aacaatcttc tcagcctgag cggacagaac gaacagaatg ccctgcagat
     4261 aaccactctg gagagtcaga ttcagactca ggctgcagat tttgataacc gtatctacca
     4321 gatggaactg caacggtacg agttacagaa agaactggtt aacactgatg tggagggcga
     4381 aatcattatc cgggcgttga ctgacgggaa agttgactcc ctgagtgtca ctgtcgggca
     4441 aatggtcaat accggagaca gccttctgca ggttattcct gagaatattg aaaactatta
     4501 tcttattctc tgggtcccga atgatgctgt tccttatatt tcggctggtg acaaagtgaa
     4561 tattcgttat gaagcctttc cggcagaaaa atttgggcag ttctctgcta cggttaaaac
     4621 tatatccaag actcctgcgt caacacagga aatgttgacc tataagggag caccacagaa
     4681 tacgccgggc gactctgttc cctggtataa agtcattgcg atgcctgaaa agcagattat
     4741 cagatatgac gaaaaatacc tccctctgga aaatggaatg aaagccgaaa gtacactatt
     4801 tctggaaaaa aggcgtattt accagtggat gctttctcct ttctatgaca tgaaacacag
     4861 tgcaacagga ccgctcaatg actaacggga atttcagaca aattataaat cagcttgata
     4921 tgcgctggcg acgtcgtgtt ccggttattc atcagacgga gaccgctgaa tgtggactgg
     4981 cctgcctggc aatgatatgc ggtcattttg gtaagaatat tgacctgata tctcttcgcc
     5041 ggaagtttaa tctctcggcc cgtggaacaa accttgcagg aatcaatgga atagcggagc
     5101 agctggggat ggtcacccgg gctctttcac tggagctgga tgaacttggt gccctcaaaa
     5161 tgccgtgtat tctccactgg gatttcagtc acttcgtcgt gctggtcagc gtaaagcgta
     5221 accgttatgt actgcatgat ccggccagag gcagaagata tctcggtcgg gaggaaatga
     5281 gccggtattt tacgggcatt gcacttgagg tctggcccgg aagtgaattc ctggcggaaa
     5341 cccagcagac ccgcataagt ctccgttcac tgattaacag tatttacggt attaaaagaa
     5401 cactggcgaa aattttctgt ctgtcagttg taattgaagc aatcaatctg ctaatgccgg
     5461 tgggaactca gctggttatg gatcatgcga ttccggcggg ggacagaggg ctgctgacgc
     5521 ttatttctgc tggcctgatg ttctttatat tgctcagggc cgcggtgagt atgctgcgtg
     5581 catggtcctc actggttatg agcacgctca tcaatataca gtggcagtcg ggtctgttta
     5641 accatcttct cagactgccg ctggcgtttt ttgaacgccg taaattaggt gatatccagt
     5701 cgcgttttgg ctcccttgac actttgaggg ccacctttac cacctgtgtt gttggggcaa
     5761 tcatggacag tattatggtt gtgggtgttt ttgtgatgat gctgttatat ggaggatatc
     5821 ttacctggat agtgctcggt tttaccatgg tttacgttct tattcgtctg gtgacatacg
     5881 gctattaccg gcaaatatcg gaagaaactc ttgtcagggg ggcccgggcc agctcctatt
     5941 ttatggaaag cctgtatggt attgccacgg taaaaatcca gggtatggct gggattcggg
     6001 gaacacactg gcttaacctg aaaatagatg cgatcaattc aggtattaag ttaaccagga
     6061 tggatttgct cttcgggggg ataaatactt ttgttgccgc ctgtgatcag gtggcgattt
     6121 tatggctggg tgcaagcctt gtgatcgata atcagatgac aatagggatg tttgtggcat
     6181 ttggttcttt tcgtgggcag ttttcggatc gggttgcttc gctgaccagt tttcttcttc
     6241 aactgagaat aatgagtctg cataatgagc gcattgcaga tattgcacta catgaaaagg
     6301 aagaaaagaa accggaaatt gaaatcgttg ctgacatgag cccggtttca ctggaaacca
     6361 ctgatttaag ctaccggtat gacagccagt cagcacaggt attcagtggt ctgaatttgt
     6421 ctgtggctcc gggagaaagt gtggctataa ctggtgcctc cggtgccgga aaaaccacat
     6481 taatgaaagt attatgtgga ctgtttgaac cagatagtgg aaaagtactg gttaatggca
     6541 cggatatacg tcaacttgga ataaataatt atcaccgtat gatagcctgt gttatgcagg
     6601 acgaccgact attttcagga tcaattcgtg aaaatatctg tgggtttgca gaagaaacag
     6661 acgacgaatg gatgacagaa tgtgccaaag caagtcatat tcatgatgtg ataatgaaaa
     6721 tgccaatggg gtatgaaacg ttaataggtg aactggggga aggtctttcc ggcggtcaaa
     6781 aacagcgtat attcattgcc cgagctttat accggaaacc tggaatatta tttatggatg
     6841 aggctacaag ttctcttgat acagaaagtg aacgtttcgt gaatgctgct ataaaaaaga
     6901 tgaatatcac ccgggtgatt attgcacata gagagacaac tttaagaaca gttgatcgga
     6961 tttttagaat tacaaattag gataatatat cactaaaagc agcattgctg cataataagc
     7021 tatgctgctt tagaaattaa tcaacttcca ctactagaag aggatgttga tgagttaaat
     7081 cctgagttta ataaacctcc attgtagttt ggattaaaac ttccaagtcc tatacccgga
     7141 ttatagttaa taccgtttga tggtattgtt attgtcctat ttgaaccatc agcaataact
     7201 cctccaatat atcctccaat tgcacttcct gctatatccg caccagggat ttttgttgct
     7261 ttacctaaaa aaccaccagc cgcagtacct attgcaacgg atgcatcttt atctaatcct
     7321 gcaccatgta ttaaagcaag t
//
LOCUS       DAZFZC010000168         7243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFZC010000168 DAZFZC010000000
VERSION     DAZFZC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.170"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..632)
                     /gene="yeeO"
                     /locus_tag="ACHKXP_004738"
     CDS             complement(<1..632)
                     /gene="yeeO"
                     /locus_tag="ACHKXP_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO7649576.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGI"
     gene            793..868
                     /locus_tag="ACHKXP_004739"
     tRNA            793..868
                     /locus_tag="ACHKXP_004739"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:826..828,aa:Asn,seq:gtt)
     gene            complement(906..1856)
                     /gene="cbl"
                     /locus_tag="ACHKXP_004740"
     CDS             complement(906..1856)
                     /gene="cbl"
                     /locus_tag="ACHKXP_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO7649577.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEI
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMESSEEEIDYQI"
     gene            complement(1958..2875)
                     /gene="nac"
                     /locus_tag="ACHKXP_004741"
     CDS             complement(1958..2875)
                     /gene="nac"
                     /locus_tag="ACHKXP_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO7649578.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3202..3277
                     /locus_tag="ACHKXP_004742"
     tRNA            3202..3277
                     /locus_tag="ACHKXP_004742"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3235..3237,aa:Asn,seq:gtt)
     gene            complement(3333..4268)
                     /gene="ldtA"
                     /locus_tag="ACHKXP_004743"
     CDS             complement(3333..4268)
                     /gene="ldtA"
                     /locus_tag="ACHKXP_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7649579.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGSDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4330..5409)
                     /gene="cobT"
                     /locus_tag="ACHKXP_004744"
     CDS             complement(4330..5409)
                     /gene="cobT"
                     /locus_tag="ACHKXP_004744"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO7649580.1"
                     /translation="MQILADLLNTIPAIDSAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGIDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSVEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5421..6164)
                     /gene="cobS"
                     /locus_tag="ACHKXP_004745"
     CDS             complement(5421..6164)
                     /gene="cobS"
                     /locus_tag="ACHKXP_004745"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO7649581.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6161..6706)
                     /gene="cobU"
                     /locus_tag="ACHKXP_004746"
     CDS             complement(6161..6706)
                     /gene="cobU"
                     /locus_tag="ACHKXP_004746"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO7649582.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6858..7031)
                     /locus_tag="ACHKXP_004747"
                     /pseudo
     CDS             complement(6858..7031)
                     /locus_tag="ACHKXP_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7010..7198)
                     /locus_tag="ACHKXP_004748"
                     /pseudo
     CDS             complement(7010..7198)
                     /locus_tag="ACHKXP_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         1979 a         1942 c         1573 g         1749 t
ORIGIN      
        1 aatatgccgc taataataat attaagaata ttcaggctac cgttaatcaa tagcggtatt
       61 ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa gagtgatggc agctgctgga
      121 taactgagta ccgtcagctc cagataagtc aacgccagtg ctttaacttc tgtcgtggca
      181 tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaataagcgt tgccaacagt
      241 acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc tcgtcgtcga
      301 tcccgcttac cgagactaaa tgccacaacg acagtagtac caagatcgat agcagcaaaa
      361 aaagccataa tgaccatatt gaagctgtcc gccaatccca cgccggccat cgcatctttt
      421 cccagccagc tgaccagaaa agtgctcaga acccccatca acaggacaca ggcattctcc
      481 atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt gtagctcttg
      541 cgtttagcgt gccagcgagt gccgtgaaca acctggcgta aagcagagga gatattcaaa
      601 gccgacctta attgcagaaa gtgaaaccac atttcaaata atgagggaga atcagcaaag
      661 ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga tagaaacggc
      721 aaacagttgg ggaatttaaa aatcgggttt gacaaaagat ttttcgccgt taagatgtgc
      781 ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
      841 actggttcga gtccagtcag aggagccaaa ttcctgaaaa gcccgctttt atagcgggct
      901 ttttgctata tctgataatc aatttcctct tcactgcttt ccatcacctg ccgcttgata
      961 tcctcaactg acagtcctgc attacaaagt tccagaaagc gccagacata gttacgctga
     1021 agttgtcctc gcttcagtcc caaccagaca gtattagcat caaaaagatg ccgcgtatcc
     1081 aggcggatta aattctcttc ctcttgttcg ccactggatt gctcggcaac taatccgatc
     1141 ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgcgcact taatacaata
     1201 tctgccagca aacctttgcg ggcaaatgcg tcatcaatac gtgagcgccc cgtaatcccc
     1261 tgtcggtaag tgattaacgg ccacttcgct attgattcca gcgtcaatgg tgaaatttgc
     1321 gtcaagggat gatcgtgtgg aacaagcaaa ctatggtgcc aacgaaacca cgggaaggcg
     1381 acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc ttcgccattt
     1441 tgcaacaatg tcgcaatttc ctgtggcgtc ccctggatta gctcgagccg aacctccggg
     1501 aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg agtatgcgtc
     1561 gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag ccgacgaaca
     1621 ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc cggttcagtc
     1681 atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaatttc atcctccagt
     1741 tcccgaatat gacggctgac gcctgactgt gaggtaaaaa gcatattcgc aacctctgtc
     1801 aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa attcacggta
     1861 aactccgggc agttcagatt tcccgttatt gttaaagtct aatgcccggc ataacaaata
     1921 ataaaaaccc gcatcttatt ccatcccgat ataacactta gctcaccaat tgccactgcc
     1981 ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact gcctgtgcct
     2041 gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa agagagactc atggaaggag
     2101 tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgcg gccgattcgg
     2161 gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca atagactcaa
     2221 tttcaccaat aacttttgcc gtgagtcgcc gtagggaaaa agcctcatca acacgaagtc
     2281 taatagcact gtaatcactg gggagaaaga ggttcatttg cgcaatagca ttcacatcaa
     2341 cgctttgccc cgggcaatct tgagttccta ccagaaaaag atcttctttc agcaaagcct
     2401 gactggatac accagccaca ggggaatgct cataaatcac cgccatatcg agttggtgat
     2461 ttatcaattt ttcgttaagc actgcaccac tattttcatg aagatagata acgatctccg
     2521 gaaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca gcggttcctg
     2581 gtgcaaagcc aatcgagact tgccccgata atgcctgacc aacgttatgc accgccagtt
     2641 gggcctgttc acactgacgt aaaatggccc gcgcatgggt atagagaatt tttccggcgt
     2701 ctgttggtgt aacgccccgc tttgtacgga tcaaaagttg ttgatttaac tcaccttcca
     2761 gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca gcagcctggg
     2821 tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag ttcatgttgc
     2881 ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagat tgcaagatgc
     2941 ttgccagttt tattttggtg ttgatgtaca agctaaccaa ctgtcaaata agagattatg
     3001 atagattcgt catttgctcc tttaatcagc tgtcgcgttc ccctgcccta taaaaggagg
     3061 gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt tgttgaaatt
     3121 gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca ctgccattaa
     3181 tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     3241 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc ctgctttcta
     3301 gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga accagaacta
     3361 atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt tacatcaact
     3421 tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac ttttcggtca
     3481 gactcatatt ctgcacgatt gcgcgacaac ggctcatgaa cttccagcca gttggagcca
     3541 tctggttcag tggtgtattt tactggctgg tcgataattt gcacacgcgt cccaacagga
     3601 acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc ctgacttacc
     3661 cggagcccaa taccaaaatt ggcattggta ccatggatgg catacaacct gccaatataa
     3721 atcgcgtaca gccccatggg attatcggaa cccgcaggaa caaatgcggg caaactctcc
     3781 cctcgtttcg catattcgcg ccgagtgttc ggcgttggcg tccaggttgg agcttcttgt
     3841 ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg gccgatacca
     3901 ataggaaaga cttccacagt attactgtct ggtgggtagt aataaagacg catctcagcg
     3961 acgttaacaa caatcccttt acgaacagtg gcgggcaaaa tcagttgctg cggaatggtg
     4021 agttgcgagc cagacttcgg caaaaaaaca tcagcgcccg ggttcgcttc cagcatgtta
     4081 cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgggtatt gtgatcagga
     4141 acagttacag taaacgactg ccccactaaa cggctaccct ctggaggtaa tggataagtt
     4201 accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat atttacacga
     4261 cgcatcatgt ccctttccta tgtcgcgaaa gctatccgtt aagtatagct tttatcagac
     4321 ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa tattactggc
     4381 agcaagttcg cccatgttgt tgtatatcgc acaggcggct tcgatgatgg gcatcgccag
     4441 agctgcacca ctcccctcac ctaaacgcat atccatattg agataaggct ccagccccaa
     4501 atgcgagagc gctatacgcg cgcctttttc taccgacaag tgagaaggaa tgagatacgg
     4561 tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa gaaatccatc
     4621 cagcaacacg ggtaaaccgc aggaagcagc acctaacatc actccagcta ttccgaccaa
     4681 atcaaatcca cccacttttg ccaggacatc aataccatcc tgaggatttg gttgattcaa
     4741 cgtaatcgcc cgacgcacaa catcaatttt attagccagt ttatctgtcg gcaggtttgc
     4801 gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta ttgctgctgc
     4861 cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac cgttttttgc
     4921 cagctcctgc gtataacata tgacgtccaa aagcaacttt tcagcctgac ggcgactcat
     4981 tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga taagcccggg
     5041 gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc ccgcttgttc
     5101 tgccagcaca cacacgccgg ttgttccacg ggtcatattt tcagcctgta tggctgtcac
     5161 ttcttttgga gaaatagcga ccccttcctc ccagacgccg tgatcggcac acataaccag
     5221 taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcctg ccagttgtat
     5281 ggcaagcgcc tccagctttc ccaggctacc aacaggtttg agtaacccgt caatatgccg
     5341 ttgtgcacgc gacatagcgg cagaatcgat ggctgggatc gtattcagta aatcggcaag
     5401 tatttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc accaagctct
     5461 atggctgctc ccagcgtatc gcccgtttgc ccgcccagcg tacgtttgag aagctgaccg
     5521 aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat acccggcaat
     5581 aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg cccatcaatt
     5641 ttgccaatga acacattgcc aagcccttct tcccgcgcat aacgatgacg atacatcaat
     5701 aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag gatcgattcg
     5761 ccacgcagag ccaactcgct taacaccaga atctttgcca gtaccacaaa aatcaatgcc
     5821 agaccgccgt gggtgcctaa acggctatca cgcatgattt ccaacatgcg atcgcggcta
     5881 cgtgcagaaa atacgccatc acaggtatca gcgaggccgt ccagatggaa tcccccggtc
     5941 atcagcacca gcacaagtac gctaaacagt gccgccagtg gtgcgccaca ccatgcctgc
     6001 agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaatcaa aggaaaagta
     6061 ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg cggtacaggc
     6121 agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt tttactccaa
     6181 tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga tttacccgcc
     6241 cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc attcccactt
     6301 cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc aacgactgaa
     6361 tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg tctttatcgc
     6421 cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattcaagc aacacaacct
     6481 cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc tccactgtgc
     6541 gccagtgctc cgggcggcct tgccgatgat gttctatccg tgcagccatc tcatcatcaa
     6601 ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc gcctctgcgt
     6661 ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg actcctgatt
     6721 caaatgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg ctgaccgcaa
     6781 aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcagt ctcaatccat
     6841 tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt ccgacaaaat
     6901 gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg aattaatgct
     6961 actgccggaa caaccagcaa acgggcattg tgttctgaaa tccaggctat tgattcaacg
     7021 tcagcgacag atctgcgctg taaaagaact tgtaaaacaa cgtaatagaa ttatccggaa
     7081 tggtggcgac tatgcactag aacaccacaa tttcgctctc tcggtaaggc actgtcacag
     7141 cattcaacag aatgtgactt gccagattta ttagcgccac cagatgttta accgggtaac
     7201 cacgacccag tcggggatat attccatctt ctcgctgaca tca
//
LOCUS       DAZFZC010000169         7227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFZC010000169 DAZFZC010000000
VERSION     DAZFZC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.171"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(700..875)
                     /locus_tag="ACHKXP_004749"
                     /pseudo
     CDS             complement(700..875)
                     /locus_tag="ACHKXP_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1064..1240)
                     /locus_tag="ACHKXP_004750"
                     /pseudo
     CDS             complement(1064..1240)
                     /locus_tag="ACHKXP_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
     gene            1300..1991
                     /locus_tag="ACHKXP_004751"
                     /pseudo
     CDS             1300..1991
                     /locus_tag="ACHKXP_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2394..2902
                     /locus_tag="ACHKXP_004752"
                     /pseudo
     CDS             2394..2902
                     /locus_tag="ACHKXP_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            complement(2920..3468)
                     /locus_tag="ACHKXP_004753"
     CDS             complement(2920..3468)
                     /locus_tag="ACHKXP_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649583.1"
                     /translation="MQITETVLSEPVEIRRFIQQAVDHWPRLLAVHFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPAPPVVLRWLREQHGGATMRCLLLLSQTSICH
                     PRVSATVDEECSQRVDLLQQTWRVISADGQCRVERCFQAARGDTSGQYIALKTVALSL
                     GLPVVTGITHRPVQRCTLITAQ"
     gene            complement(3669..3905)
                     /locus_tag="ACHKXP_004754"
     CDS             complement(3669..3905)
                     /locus_tag="ACHKXP_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7649584.1"
                     /translation="MLTSMTGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEASRGAAA"
     gene            complement(3974..4546)
                     /locus_tag="ACHKXP_004755"
     CDS             complement(3974..4546)
                     /locus_tag="ACHKXP_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649585.1"
                     /translation="MNAIPYFDYSMAPFWPSYQNKIIGVLNRVLREQSCSRIRRILLR
                     LPGEHGNIFSDRKTWFGMAFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSCSPDCEERLKELIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQL"
     gene            complement(4903..5634)
                     /locus_tag="ACHKXP_004756"
     CDS             complement(4903..5634)
                     /locus_tag="ACHKXP_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7649586.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIKLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSDTRKASDKKPGEAAVLWIKRGDVEALQKARNRASYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT         2062 a         1768 c         1578 g         1819 t
ORIGIN      
        1 cagcagaaaa cgactggcat taaaatcatt aaattataaa catatacaac tttgtaaagg
       61 tggttataat aaacctgatt actttaacgt ctgtatacct gcactgctga tatcacaaaa
      121 caataaaaat cctggttaat atctctgatg attgaatgag aaattcacga tgtctggtga
      181 gaattctgtt caaaacgagg aatgcagacg tacatgtggc aggcatggac ttctaccaat
      241 ttaacttttt tccttccttg tatttacaca ttgcacattt tgcgctacaa aaatttcacg
      301 tggcacgcac cacgtgatcc gtagaaaagc ctcttgttac gaatttcgta aaccgcacgt
      361 ctgtctggtc gtgcggattt tttatccgtc aacttcacat gaaacccgcc agagcactgt
      421 ttccggattc agacatcagg catattatca tccacgatgt cgtgaacaag atcacacttt
      481 tgcaattcct ttattgatcg aaagcagtta atcacggaaa ttaattaccg gagtgaaagc
      541 attactgcat cgtaaatatc taccattttt gactacaccc tctgacagaa ttttcagagg
      601 gtttttttat cagtttcacc atccaaccct gtacgccaga atggtttcac tgtcactcag
      661 ttctgctatt gcctttttca tctcccttta ccttacgttc tgccagagtc tgagccattt
      721 gaacaggagg ttatcattga tatcgtgttc ccgggcaata cgggcaacag aggctcctgg
      781 ttgcaaagcc agtttaacca tttgaagttt aactcatttg aaaatgatct gcggggttct
      841 gcggataatg tttttgctcc ataacaggcg cccaccagtt tgaaaaggtg ggcacctacg
      901 ttaccaatac tggcttaatg gatacatacg gcggtcggtt cttacggtaa aacgacacaa
      961 ccaaacgctt cttatcctgc aatgaactgc tttattaccg agtttagtga ccgcacagac
     1021 actcactttt aagaaaaaca gaatggtaaa caaactccat gaattacaat agctctctgg
     1081 ctgccgccac aatagcttgc gcggttaacc cgtactctct ctgtaaaaag tcctgagttt
     1141 caacctggcc ataccgctct ttaaccccca cccgacgcat gggcacagga caagtttcta
     1201 caagcacttc agcaactgct gaacctaatc cattatgaat acggtcatga ctccaattag
     1261 ttgaattagg ggtataatag ccacttttaa ggttctttca tggtcacagt taaccttcat
     1321 tgctcctgtt gccagtccgt tcaggtttac cgtcacggga agaacccaaa ggacatgatc
     1381 gatttcgctg ccgtgactgt catcgagtgc ttcagctcac ttaccgttac gaggcccgta
     1441 aaccgggagt taaagagcaa atcactgaaa tggctttcaa cggtgccgga gttcgcgata
     1501 ctgcccgtac gctgaaaatc ggcattaata ctgtcatccg gactttaaaa aactctcgcc
     1561 acgaagaata acgtcatacc cggtcgctca tgctgatatg gctctcatct gcgaacttga
     1621 tgagcaatgg agttttgtcg gcagtaaagc gcgacagcac tggctctggt acacgtacaa
     1681 cacgaaaact ggtggcgttc tggcctacac ttttggccta cggactgatt aaacctgtcg
     1741 tgaattgctg gcgctgcttg cccttttacc atcggtatgt tgaccactga cgaatggggt
     1801 agttacacca gagagttacc gaaggagaag catctgactg gtaagatttt tactcagcgc
     1861 attgagcgca acatctacct ctgagaaccc gaatcaaacg tctggcccgc agaactatct
     1921 gtttctcgcg ctctgtagaa cttcatgaaa aagtcatcgg tgcctttatc gaaaaatata
     1981 tgttctacta attggagtca ccaccttcgt ttgtttaaag aacctgctgg tcctgccatt
     2041 aatattacct gctgattgct tcacatccgg attcctcgca ctgcgcgcgg gttattgttt
     2101 cgttttcggc cacggggtcg ccagggctct gtttgcaggc catgatggcc tgacctgtac
     2161 tgtttctcag gcactgagat gtccgtttac gccatttgtt cccggatatc cttaacctct
     2221 gacgtttgtt ccctgcccct ctgtctgata acgaatacgc tcacaacgca cagtactgaa
     2281 caataccgac aacaacatgt cttacatgaa attcgctgta acatccaccg gaaaacattt
     2341 tcagatatcg gatgaggtac tacttccatt gacagcgcga ggtaacaaaa ttgatggtaa
     2401 ctctgaccat taaccgtaaa ccgcagggta ttttcagtaa atcaccggca acgccgctgc
     2461 aggataaaac ggacacagca tacaaaatga aggccggtga ccagatacca cagttgaacg
     2521 ataaacagaa acctgctgat aaaacaccgt ggcgacatga caaaacgcca gagcaaaaac
     2581 cgtaagcgca ttattcgtct tgtggaactc tggcctgagt cattcaaccg ggaaaaaaac
     2641 aaaaccactt aaagtgggga tacctgacga cctgatacag gatatcgcca tccgagaact
     2701 ggcgtttggc gctggtacat tacatgcagc agtggcgtct tatgtgcagt ctccgcgcta
     2761 ttaccgtgcg ttaatagcag gtggcgcccg ttacaacctg aacggtcagc cgtgcgggga
     2821 agtgacacca caggaacaga aagaggcaga gacccgttta atgatgctga atgaccgacg
     2881 aaaagacaga aaacctcgtt gactgcacaa cagcgctgtt cactgagctg taatcaatgt
     2941 acagcgctgt accgggcgat gggtaatacc ggtcacaacc ggtaacccca gagacaatgc
     3001 aactgttttt aacgcaatat actggccgga tgtatcaccc cgggcagcct gaaaacacct
     3061 ttccacccgg cattgtccgt cagcacttat cacccgccat gtctgttgca ataaatccac
     3121 ccgttgcgaa cattcttcat caaccgtggc actgacccgt ggatggcaaa tgctcgtctg
     3181 gctgagcagc aacaggcatc gcattgttgc tcctccatgt tgttcccgca accagcgtaa
     3241 taccaccggt ggtgctggac tggccgtgtg attacgttca gtaatacgct cgtgcacctg
     3301 tcggtaaaag gaagtacaga atgcatgaat ctgttgtccg ttgatgtttc cttctgccga
     3361 atggagtgtg aagtggacgg ccagcagacg cggccagtga tccaccgcct gctgaataaa
     3421 acgccggatt tccaccggct ctgaaagtac ggtttcggtt atttgcacga tttgatcccc
     3481 ttgaatgttg ctgaaccggt gtcggtacgc aggaaagcac gccgagaccg gtacgatgcc
     3541 tgttacagcg aagaacgagg aatgacagac gggggcagca ggtcgccagc accagggaca
     3601 gggctgattt ccacaatacc aacgctggta ttttgcacat tgccagcgta ccggcattac
     3661 aacacgtttc atgctgctgc ccctcgtgat gcctcgattc gttgctgcat ccactgctcc
     3721 acttcacttt tgtaccagcg gctgctgcgc cccagtttga tgggtttagg gaaatggcct
     3781 tcactgatca gcttgtaaaa ccatttgtcg gtcataccag tgaagctggt gatgtagttc
     3841 atgtcgatca ggggatcgtc ggcacgcagc aatacgctgt cgtgacctgt cattgaggtc
     3901 aacatggtca gaatgtcctc tgtgagattt atgaatgttg ggcaaatgcg ggtgaatctg
     3961 ctgagctgag ctttcagagc tggcctgcta cgggatacca gggattaccg gcgaggtaac
     4021 gggcttccgg atcgcgcaca ccacgacgat cgcgggcgaa atcactgaac gcccatatca
     4081 gctctttgag tctctcttcg cagtccggtg aacaactgtc cagcaccaca tcccgggtta
     4141 tctgtttctg gtacggtgct gcttcacccg gtgacgcgaa gcccagtacg ttgctccagg
     4201 catccagaat caaccggtcc agctcagggc cgtcgaaata ttcctgtctg acgcacagca
     4261 ccacgtggta ttccggtttt tcaggatgac gtgtcaggag ccagcagagg tcgcgtccgg
     4321 gggttgcatt catcagcgca ctgacggttt cgataaaggc cataccgaac caggtttttc
     4381 tgtcgctgaa gatgttgcca tgttcccccg gcagacgaag caggatccgc cgtattcggg
     4441 agcaggactg ctcgcgcaac acacggttga gaacgccgat gattttgttc tgataagatg
     4501 gccagaaggg cgccatgcta tagtcgaaat aagggatagc attcataaga tatgttccgt
     4561 tatacgcagc ccacaacgcg tcctgcccgt ggatgcgctg acgtgttatg gcttgcgtat
     4621 ggggttgatg gaataagtga aaaaccgcgg cagaacggga agatcaggta accgacaagg
     4681 gacagaacac cggatgcagg ttcagaatga atgctctccg ttgacttcgg ttcacatcat
     4741 taagcaggac agtaaggttt taccgacgga gtgtttccag gagcgaaagc agagaaaaat
     4801 accgttgcat gacttcagga cgactttttc agaaaacgag ctaagggcgt cagccaacgt
     4861 cctgtagcat aagctcagac accggatgtc gttttacagg gctcacctgc catccagcag
     4921 acgccccggc ccacgactgc aaccataatt acgttgcccg gaaccgtcat gctgcttcgt
     4981 ctcatatttc acgagataac tggctctgtt ccgggctttc tgcagcgctt ccacatcacc
     5041 acgcttgatc cagagaacag cggcttcacc cggctttttg tctgaagcct tcctggtatc
     5101 agaacatcca cccgacaaag gcgtatcatc ggaagaaggg cgagcatcag atgatgtcgg
     5161 tttacggaaa ggcgtccgcc tggaaaaatg aaccagtcca ttaccctgcc agggctcaag
     5221 atgcagagca ctacaccatg ccagcttgat cagtgtcgcc agcgaagaag gaacggtgaa
     5281 atcccctggc gagcaccagg tatctttgtt gagcagtaat atcacgtgat aatgtttcct
     5341 gcctttttcc ttgccaaact cccgacacca gaaataacgc agagtggttg cacgcacacg
     5401 cttgttggca cgtcgtttat gtcgctggta agcctgaatt tttgctttca gggatgcaaa
     5461 aaaacgagaa atcactgctg aatctaaatc ggtatccata atagtggcag gcatatactc
     5521 aggaaaacgt aaatccaccc ggattaacga gacggcacgg tgttgcgcca gggcactgtt
     5581 gagaatactt tcaaaacggg ataaccagta agcattgtgt attggttgat tcatgatgag
     5641 agatctccgt tgatttggat tgacttcaca tcattaatca ccgcggtaaa actttacggc
     5701 atccggcatt gagcaatgct acaactctct gattaagagt gattgaaacg gaacaaacca
     5761 tcccggaacg aaaagataat tctacacgca gggtaaaaac actcaagaac agcgaatgga
     5821 tttaaaaacg acatggacaa cgagaaaagt cagaagggaa aaggatccac agaacattcg
     5881 aacaaaacac cagaaacgca accagaacgc ttcctggtaa tgcaaatcaa caacaacccc
     5941 aaaacctaca gcttaatcac acaactcaat cacggatgcg aatgacacgt ttaaaacctg
     6001 tttacagata aaccccgcca caagaacaaa aatcaccaac atcagaatcg attttctcat
     6061 cctgaaaaag ttacgtttca ctacgcacca taaaaacgat taaccatcga taacgtaaac
     6121 cttatctgaa tcaaaaacac caaaaccgtt catcacactt tcgcaatgca agaagaaacc
     6181 tcatgacaaa aagaatgacc ttacacggaa aagaaaacgt caggaatcgt taatgcctca
     6241 atctgattgt tatcaaaaca tccagaaagt cgtttaacag aatcgtcgta agcacatcga
     6301 aaaaaacgtc agattacttc aggaaagttc gggttgattt agcgtaaaga tacttttctc
     6361 atgctaacgt cgaaactcga aatctcagaa aaggttaaac gcaacatcag taaaatcgaa
     6421 tagcagattg attgcttaaa cagaaaggtt ttacagcagc tttaaactga ttttagctaa
     6481 cgtaaagatt tactaaactg gatgtgtttc agacaaagca atgtgtacag caatgcttaa
     6541 tgaaaacact tatagggttg tttatgaaaa cacgtgtatg tctggtaaaa cacttactga
     6601 aatgcttagt aaaaatactt agtaaaatac gtgtatatag tatcataccg acattagcaa
     6661 cattctcaga cactcatgca gactgaatgt tgattacccg ttttcacatc aggcatggtt
     6721 ctttactgac caaaaaacaa actggtttac tcttgaatta ttttctctac ctcagaaaac
     6781 atggtctcag gaatctgcta tggtttcata gtttctgttg ttcttttcat aaaagttata
     6841 agtcattgtc ataatcatac gataatcact ttatgttagt ttttactgtc acacgctact
     6901 gactttttca gtcattgcca gaaaggggct ctttttactt ccatttcacc actcaacaaa
     6961 tgcaaaactt gtccagtaaa gcggaccacc tcttgctcac aagcaacgaa tcatctccag
     7021 gaacagccct caaacattgc agaaacaaaa acaacaaaac cctcattatc actgatgttc
     7081 ggaaaatttc ccgaaacaaa aaaaacgaaa tcaaaatgag actcattatc atggatgtag
     7141 tcaaaaagac gttatggtgg ctttcagtta aatggtacta tttctcataa atccacttaa
     7201 ctttttttac tgattcatcc cagataa
//
LOCUS       DAZFZC010000170         7193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFZC010000170 DAZFZC010000000
VERSION     DAZFZC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.172"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..707)
                     /locus_tag="ACHKXP_004757"
     CDS             complement(<1..707)
                     /locus_tag="ACHKXP_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO7649587.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKL"
     gene            complement(885..1796)
                     /gene="gsiD"
                     /locus_tag="ACHKXP_004758"
     CDS             complement(885..1796)
                     /gene="gsiD"
                     /locus_tag="ACHKXP_004758"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO7649588.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTVLLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1799..2719)
                     /gene="gsiC"
                     /locus_tag="ACHKXP_004759"
     CDS             complement(1799..2719)
                     /gene="gsiC"
                     /locus_tag="ACHKXP_004759"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO7649589.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2737..4275)
                     /gene="gsiB"
                     /locus_tag="ACHKXP_004760"
     CDS             complement(2737..4275)
                     /gene="gsiB"
                     /locus_tag="ACHKXP_004760"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO7649590.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFYPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4295..6166)
                     /gene="gsiA"
                     /locus_tag="ACHKXP_004761"
     CDS             complement(4295..6166)
                     /gene="gsiA"
                     /locus_tag="ACHKXP_004761"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO7649591.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFSVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLFNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6153..7118)
                     /gene="iaaA"
                     /locus_tag="ACHKXP_004762"
     CDS             complement(6153..7118)
                     /gene="iaaA"
                     /locus_tag="ACHKXP_004762"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7649592.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
BASE COUNT         1823 a         2043 c         1673 g         1654 t
ORIGIN      
        1 agtttttcac gagcgagttg attcttggca atgatctcat ggccttgttt caaaaataga
       61 acttcatcat tagcgctgat ttgatcaaga tactcgttca gatatcgcca gtttaaatcg
      121 acatgaattt tatccaccgc taacaaatcg tgatctttgc cgttgatgtg tagcgtaaaa
      181 gagatacatt ttttacccgg acgatcaaag caattgtatg ccggattcca gctgttgtta
      241 gagtcatctg aagccaccga ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc
      301 cgccctgact cgtcgacaag cggtccattt acttgttcct gatgataaac aacgatttta
      361 tcattcgccc tccgcgaaaa aagataatat ttattatcat caagtaccag tcccaccgag
      421 tctaaaaatg gcatcatttc cattttaaga atcacggcgc gttgaatctc ctctgtattt
      481 tttaataaat acagtgagtc ctttaatgtc agggccgaac gttcaatatc tttaaagaaa
      541 tagccaacgt ttttctgaat aagttcaaag cggctatcgg cacgttttac cgcgcgctcc
      601 gataccagtt gatagttaat gatgaaaaaa agaccaataa atgacagtgc tgccaataaa
      661 aataaaatta tcagccttat ctttatcttt tcgtataaac tcagcatgtt aatacattta
      721 acaaaataac tatctgaacg ctgtgccgct gcaaaaattg tcccttgctg cggcttttta
      781 accaaattac ccgattctac aaaatcagca gaaagttaca acacttgctt agggcaataa
      841 tttcctgtga ggtgattacc ctttcaagca atattcaaac gtaactatcc tttaattttc
      901 ggatccagcg catcgcgtaa accatcgccc aacaaattga acgccagtac ggtcagaaaa
      961 atagccaggg ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc tcgagcctca
     1021 ttgagcattg ctccccactc tggtgtcggc ggctgcgcac cgaggccgag aaatgagagg
     1081 ctggcggcag agataatcga ggtaccaatg cgcatggtga aaaacaccac gatagaagag
     1141 acggtcccag gcaggatatg acgcaacaaa acggtcatat cgctggcacc aatactgcgt
     1201 gctgactcaa taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac caggcgggca
     1261 aacgcgggga tggaaaaaat ggcgactgca ataatcacgt tagcaatgcc gcttcccaac
     1321 accgcaacaa cagcgatcgc cagtaaaata cccgggaagg caaacagcac atcgcaaatg
     1381 cgcatgatca gccgatccca ccagccttca taatatccag cgagcaagcc cagcaacgtc
     1441 ccgatcgccg caccgataaa cacggcaaac acgcccgccg ccagcgagat ttgcgcacca
     1501 accaggacac ggctgaaaat gtcacgcccc agtgaatcga cgccaaacca gtgctgcaaa
     1561 gaaggtccgt tattcagatt gtcataatca aaataatttt cggcgtcata gggagcgatc
     1621 cagcgtgcaa aaatggccac cacaatcaat aaaataacga ataatgcggc ggtcatcgcc
     1681 atatgctgac ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac ctggtcaggt
     1741 ttgaccagtg gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt
     1801 acttgtacct gatagccggg ttaatggcgg cgtaaagcac atccaccact aagttgataa
     1861 gaataaattc cagcgagaaa agcagaattt ccgcctgaat caccgggtaa tcacgcattt
     1921 ctacggagtc aacgagtaag cgtccaagtc ccggccagtt gaaaactttc tccacaacga
     1981 tggaaccacc gagcaaaaag ccaaactgta agcccatcat ggtcactacc gggatcatcg
     2041 cgttacgtag cccgtgtttg aggacaaccc aggtttcgct cacccctttc gccctcgcgg
     2101 tacgcatata atcttcgctt aaaacatcga caaacgacgc gcgggtaaag cgcgccatca
     2161 cggcggccac tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc caactgtctg
     2221 ctcccacggt aggcagccag cccagttcaa cggagaatac ctgaattaaa agcatcccca
     2281 gagcaaatgc cggaaacgag atccccgaca ccgcaatggt catactcaat cgatccggcc
     2341 aacggttacg ccagacggcg gcgataattc ccgccgccat accaaatata accgcccaga
     2401 ccatactggt tatggtcagc cacagcgttg gcataaagcg gctggcaatc tcatcggcaa
     2461 ccggacgacg cgacaccatc gacaggccaa aatccccctg cacagcattg ctgatatagt
     2521 gccagaactg gtgatacagc ggctgatcca accccagctg ctgacgcacc agttctataa
     2581 cctgcgcatc agcttcgggc ccggcaatca atcgcgccgg atcgccgggc agcatatgga
     2641 caaataaaaa caccagcacc gagacgataa acagcgtcgg aatcaacccc agtaagcgtt
     2701 tgataacgta attaagcatt ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa
     2761 gctgaagccg gtgtctggca tgatccaaaa accggtcagg tttttactgt gtgccgacac
     2821 cagtttttct accaccagcg ggatccacgg cgattcttgc cagatgatat cctgcgccgc
     2881 cttatataag cgggtctttt ccgccggatc attagttttc agtgcctgag ccaggaagtc
     2941 atccacctgt ttattgctgt aaaacgcggt attaaacagc gtcggtggcc agttctgcga
     3001 ggcaaacagc ggcgatagtg cccagtccgc ttcgccggtt gaagccgacc agccagtgta
     3061 gaacatccgc acgccgctct ctttttgccc tttaccttca acttctgccg cccgctgtcc
     3121 ggcatccatc gcagtcacct gggctttaat cccgacctgc gctaactgct gctgggtaaa
     3181 ttgcagcact ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc
     3241 gttgggatat cccgcctctt tcagtaattc gcgcgctttc actggatcgt aaggccacgg
     3301 tttataactt tgcgcgtagg cgatacttgg cggtaccaca ccagtagctg gcgttgcata
     3361 gcccgcaaag gcaactttca ccagcgccgg acggttaatg gcgtaattca gcgcctcacg
     3421 gaccttcggg ttatcgaacg gcttttgcgt cacgttcata ctgatataac gctgcataat
     3481 tgacggactg gccatcaact cgatattttt gtttttctcc agcagtgtgg cctgctcgta
     3541 aggaatgggg aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt
     3601 atccgccacc ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc
     3661 cgcgaatttt ttcaccttca caaaatcggt ctgattccag gtatccagtt catacggtcc
     3721 ggttcccacc ggataaaaac caatctcctt gccatatttt tccagcgctg ccggtgaaat
     3781 catcgcggtc gccggatggg caagaatatt aataaacgct gagaacggct gtttgagggt
     3841 aatctttacc gttgtcggat cgatcgcttc cgttttagca atattcttat acaggttata
     3901 gcgtttaaga tgattcgccg gatcgctggc ccggtccaga ttcgctttta ccgccgctgc
     3961 gttgaaatcg gtgccatcct ggaatttaat tccttcccgc aatttcacgg tgtaagtaat
     4021 gccgtcatcg gaaacggtat aactctccgc cagcacgttt ttcagtttca tctctttatc
     4081 cagaccgaac agcccctggt aaaacgattt cgctacggcc tgagataacg tgtcattcgc
     4141 gtcatacgga tcgagcgtgg tgaaattcga tcctaccgcc accaccacat ctttggcagc
     4201 gaatgcacaa gatgccatca acgctgtcgc aatgcccagc gccactaacc cactacggtg
     4261 tacagctctt gccattttat tctccgcctg aatgttatct acgcatgaat gcgtattctg
     4321 attgtggttg tgcgacgtaa tgccccggcc cgacgcattg caacgagacg gctgccacct
     4381 cttcgccacg cagatgaata ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc
     4441 gttgtcggga cggttcagcg accggaactg ccgccagtaa tttacgcgta taaggatgct
     4501 gcgggttttc gaagaccgcg cgccgtggac caatttcaac aatttgcccg agatacatca
     4561 ccgccacacg atgactaatc cgctctacca cagccatatc gtgggagata aacagatacg
     4621 caatgccgaa atcacgctgg agatcgagca acaagttgat aatctgcccg cgaattgaaa
     4681 catccagcgc cgaaacggct tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc
     4741 gagcaatgca gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat
     4801 gttcaggtaa caggcccacg cgctccagca accacgcaac gcgtgcagcc gcgtctttac
     4861 ctggcaataa accgtgtaca cgcagcggtt cgataatcga atcaccgatg gtctgacgtg
     4921 ggtccagcga agcgtaaggg tcctgaaaaa taaactgaat atcgcggcgc aatgcctgaa
     4981 gtttgccggg tgacaaggta tcgattcgct gaccgttaaa gataatttcg ccgccctgcg
     5041 attcgaccag gcgcagcaac gcccgcccgg tagtggattt accgctgcca gactcgccca
     5101 ccagcgatag cgtttcgcca ggccagagat caaaactgac tttctcaacg gcatgcactt
     5161 cccgcgttac gcgattaaac aaaccgctgc gcaaagggaa acgggtgacc agattacgca
     5221 cccgtaaaac aggttcacca tccaccaccg ttttctgctc gatggggggt tcctgtttcg
     5281 ctggatgttc aagcgatatc aatgggaaac gtcggggata atctaaccct ttcatcgcac
     5341 caagttgcgg aacagcagct aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa
     5401 tctgttcgac ggtacccgtt tccaccgcct cgccctgata catcaccagt acccgatcgg
     5461 caatctctgc caccacgccc atatcgtgag tgataaagat aacgcccatc gacatctctt
     5521 tttgcaatac tttgattaat tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg
     5581 ttggctcatc ggcaatcagc accgccgggc ggcatgacag cgccatcgca atcatcactc
     5641 gctggcgcat cccgccagag agttgatgcg gataacgtga aagaatggtt tgtgcctccg
     5701 gaatgcgtac ctgatccagc atccgcttcg cctcgaccat cgcttcttca cgactggcgt
     5761 tctgatgcag acgaattgat tcggcaatct gttcacccac agaaaatacc gggttcagcg
     5821 atgtcatcgg ctcctgaaat atcatcgcca tatccgcacc gcgcacatgg cgcatttgtg
     5881 cagcgctctg ctcgctaagt tcaatcacat cacgactgcg ccgccgcaac agcattttat
     5941 cgcactgtac taaaccgccc gcctgttcca acagacgcat caatgccagc gcagtcactg
     6001 acttaccgga gccggattcg ccaacaattg ccagcgtctc accgcgttgc agactaaaag
     6061 agagattgcg gaccgcagct attttctgct ggtcctgcat aaaggcaata ttaagatttt
     6121 caaccgccag cacattaccg gcatcaagtt catcactgtg tggcaacggt gtcccctttt
     6181 tcacggtaga taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg atacattcct
     6241 tcggtgttaa acggtagcgc gacattccct tcatggtcga tagcgattaa gccaccgcta
     6301 ccgccaagcg cagggagttt ttccattact acccgctcgc aggcttccgc gagacttaat
     6361 ccgccgtaat ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg
     6421 cccgtgccgg tacaagaaac cgccacactg gcgttattgg cgtagcatcc ggcacccact
     6481 aagggactat cgccaactcg tccgggtaat ttattggtca ttccgcctgt ggacgtggct
     6541 gccgccagat tgccgtctaa atccaacgcc acggccccca cggtgcccat tttctgtttt
     6601 tcatccagtg gcgcaccgct atggtcgagg actgttgccc cttcctcgcg cgctgccagt
     6661 agttgttcat aacgcaaagg cgtggagaaa atctccggtg agacgcgctc catgccatga
     6721 gcaaacgcaa aattttctgc cccttcgcca atcatcatca catgcgggct ttgctccatc
     6781 accagccggg cggcaagaac cggattacgc agatgactaa cgcccgccac cgcaccggct
     6841 ttcagggtgt taccatccat cacacaggcg tccaattcat gggtctcatc acgcgtaaag
     6901 acagcgccaa ttccggcgtt aaacagtgga cactcttcca gcagacgcac cgcttccgtc
     6961 accacatcca gcgcactttc gcccgcttcc agcattttct gcccggtttc aacaatggca
     7021 gacaacgcct cgatgtagcg taattcctgt tgcagactca tctgcgcgcg gctaattgca
     7081 cctgcgccac catgaattgc aatgactgct ttgcccatag tattcgtcca ttaagggtgg
     7141 ctcgccactt ttgctataaa tatcagaatt tttatgaatt actcattcaa ttt
//
LOCUS       DAZFZC010000171         7113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFZC010000171 DAZFZC010000000
VERSION     DAZFZC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.173"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..1422
                     /gene="adeQ"
                     /locus_tag="ACHKXP_004763"
                     /gene_synonym="yicO"
     CDS             88..1422
                     /gene="adeQ"
                     /locus_tag="ACHKXP_004763"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO7649593.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAIVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1475..1927
                     /gene="yicN"
                     /locus_tag="ACHKXP_004764"
     CDS             1475..1927
                     /gene="yicN"
                     /locus_tag="ACHKXP_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO7649594.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2138..3328
                     /gene="nepI"
                     /locus_tag="ACHKXP_004765"
     CDS             2138..3328
                     /gene="nepI"
                     /locus_tag="ACHKXP_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO7649595.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWCNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3369..3662)
                     /gene="yicS"
                     /locus_tag="ACHKXP_004766"
     CDS             complement(3369..3662)
                     /gene="yicS"
                     /locus_tag="ACHKXP_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO7649596.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3884..4702
                     /gene="nlpA"
                     /locus_tag="ACHKXP_004767"
     CDS             3884..4702
                     /gene="nlpA"
                     /locus_tag="ACHKXP_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO7649597.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4706..5629)
                     /gene="yicL"
                     /locus_tag="ACHKXP_004768"
     CDS             complement(4706..5629)
                     /gene="yicL"
                     /locus_tag="ACHKXP_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO7649598.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHK"
     gene            complement(5740..6924)
                     /gene="setC"
                     /locus_tag="ACHKXP_004769"
     CDS             complement(5740..6924)
                     /gene="setC"
                     /locus_tag="ACHKXP_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO7649599.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1788 a         1599 c         1792 g         1934 t
ORIGIN      
        1 aattgaaatg tgataattat cactgaaccg ttgttgtcat ttgttttaca aaagcaaggg
       61 attaaatctc ctccaatgga caaaaaaatg aataatgaca ataccgatta cgtgagtaat
      121 gaatcaggga cgctttcgcg attatttaaa ctacctcagc atgggaccac cgtccgcaca
      181 gaattgattg cggggatgac cactttttta accatggtgt acatcgtttt tgtgaacccg
      241 caaatcctcg gcgcggcaca aatggacccg aaagtggtgt ttgttaccac ctgtttgatt
      301 gccggtatcg gcagtattgc gatggggata tttgctaact tacccgtggc gctggctccg
      361 gcaatggggc tgaacgcctt ctttgccttc gtggtcgtgg gggcgatggg catctcctgg
      421 cagaccggga tgggcgcaat attctggggc gcagttggac tatttttgct cacgctgttt
      481 cgtatccggt actggatgat ctccaacatt cccttaagtt tacgtattgg tatcaccagc
      541 ggaattggat tatttatcgc cttaatggga ttaaaaaata ctggcgttat tgtcgccaat
      601 aaagacacgc tggtgatgat tggcgattta agttctcacg gcgtgttgtt aggtatttta
      661 gggtttttta ttataaccgt gttgtcatca cgtcattttc atgccgcggt gctggtttct
      721 attgtggtga cgtcttgctg tggattattt ttcggtgatg ttcattttag cggcgtctat
      781 tccattccgc ctgatattag cggcgtcatt ggtgaagtag atttgagcgg cgcgttaaca
      841 cttgaactcg ccggtatcat tttctccttt atgctgatca acctatttga ttcatcagga
      901 acattaattg gtgtaactga taaagcgggc ttaatagatg gtaacggtaa attccccaat
      961 atgaataagg cgctgtatgt tgatagcgtc agttcggtgg cgggtgcgtt tatcggcacc
     1021 tcgtctgtta ctgcctatat tgaaagtact tctggtgtgg cagtcggtgg ccgcacgggg
     1081 ctgactgcgg ttgtggttgg cgttatgttc ctgttggtta tgttcttctc accgctggtg
     1141 gcgatagttc ctccttacgc aaccgccgga gcgttaatct ttgttggcgt gctgatgact
     1201 tcgagcctgg cgcgcgttaa ctgggatgat tttaccgaat cggtgcctgc gtttattacc
     1261 acggtgatga tgccctttac tttctcgatc accgaaggga ttgcactcgg ctttatgtcg
     1321 tactgcatca tgaaagtatg caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg
     1381 gtcgcagctc tgtttgcact gaagattatt ctggtggatt agtaactggc gagggtttcc
     1441 agatatcatg agttctgatt acgcaggaga actcatgatc tggataatgc tcgccacgct
     1501 ggcggtagtg tttgtggttg gttttcgggt gctgacatcc ggggccagaa aagcgattcg
     1561 ccgtctcagc gatcggctga acatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat
     1621 gggaaagtca gctggtgacg aatttttacg ttatttgcat cgtccggatg agtcgcacct
     1681 gcaaaacgcc gcgcaggtgt tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca
     1741 gaacctgctg caatggcatc ggattttaca aaaagctcgc ctcgccgcgc cgattaccga
     1801 cgctcaggtc aggctggcgc taggttttct gcgcgaaacc gaacctgaaa tgcaggatat
     1861 taatgctttt cagatgcgct ataacgcgtt ctttcagcct gccgagggcg ttcactggtt
     1921 gcattgaaac cgtttcgtct tatcagccta cagaccgcgc ctgacctgtc gggtggataa
     1981 gacgttcacg ccgcatccgg taatctttgc cctgttccgt ttcgatctta actccagctc
     2041 aagcaacacc gtaaaagttg caaaaacgtt aaatgcgtca cacatttcaa tgttaaagtg
     2101 gtcggctttt cccctgaaac atgccacggg taacaccatg agtgaattta ttgccgaaaa
     2161 ccgcggcgcg gatgccatca cccgaccgaa ctggtcagcc gttttctcgg tggcgttttg
     2221 tgtcgcctgt ctgattatcg ttgagttttt gcccgtcagt ttgttgacgc caatggccca
     2281 ggatttaggc atttcggaag gggttgccgg gcaatcggtg accgtgaccg cctttgtggc
     2341 aatgtttgcc agtttgttta ttacccagac aattcaggct actgaccgcc gctacgttgt
     2401 tattttgttt gccgttttgc tgacgctctc ctgcttgctg gtttcctttg ctaactcatt
     2461 cagtttgctt ttaatcggtc gtgcctgtct ggggctggcg ctgggcgggt tctgggcgat
     2521 gtcggcgtcg ctgaccatgc gtctggtgcc gccgcgtacg gtgccgaagg cgctgtcggt
     2581 gatcttcggc gcggtttcta ttgcgctggt gattgccgcg ccgttgggca gttttttagg
     2641 cgagcttatc ggttggtgca atgtctttaa tgcggcggcg gtgatgggcg tgctgtgtat
     2701 tttctggatt atcaaatcat tgccttcact gccaggcgaa ccctcgcatc agaaacaaaa
     2761 tactttccgc ttattacaac gtccgggtgt gatggcaggg atgatcgcca tcttcatgtc
     2821 tttcgccggg cagtttgctt tcttcacgta tattcgcccg gtgtatatga acctggcggg
     2881 attcggcgtg gatggcttaa cgctggtgct gttgagtttt ggtatcgcca gctttattgg
     2941 tacgtcgctt tcgtcgttca ttcttaaacg ttcggtaaaa ctggccttag caggcgcgcc
     3001 gttaatactg gctgtgagtg cgttggtact gacgttgtgg ggaagcgata aaatcgttgc
     3061 taccggcgtg gcgattatct gggggctaac ttttgcattg gttcccgtcg gctggtcaac
     3121 gtggatcacc cgctcgctgg ccgatcaggc agaaaaagcc gggtctattc aggtggcggt
     3181 tattcagctt gctaatacct gtggcgcggc aatcggcggt tatgcgctgg ataatattgg
     3241 tctgacttcg ccgttgatgt tgtccggcac attgatgttg ctgactgcat tgttggttac
     3301 tgcaaaggtg aaaatgaaga aatcctgata tcaggtaaag aagcgttggt cagtagttgt
     3361 tgctgttctt acatatccgg gcattctacc ttacccaacg cttcccaata attgctctga
     3421 ttattacgtt ccatcgccac gatggcctcg cgtatatgtt gtttattgtt ttcatctgac
     3481 aacattaact tttcccacgc ttcatctgat aatgacgcct gtggcgtgca aattttacgt
     3541 aaatcttgta ataccgtggt tttctctttt gcggactgca gtgagctaaa aggcgactcg
     3601 gcgtatacga tgcctggcat agcgaaaaaa gtgaaaataa gcagtagtgt cgttggtttc
     3661 atggattcaa ccttttacaa atgtgtatcg aatgaagcat aagtgtagtt tgctttcatc
     3721 gctctcatca tgctgtacga aacgcgcttg cgtggtcagt aagaagtgcc agactttata
     3781 ttccactttt attccttttt attcttatcg atagcgtttc gttttttaaa ccgcagcgac
     3841 cttaccgcta tagtcaggta atcattaata aaaggataaa aaaatgaaac tgacaacaca
     3901 tcatctacgg acaggggccg cattattgct ggccggaatt ctgctggcag gttgcgacca
     3961 gagtagcagc gatgcaaaac acattaaagt tggcgttata aatggcgcag aacaagatgt
     4021 cgcggaagtc gctaaaaagg tggcaaaaga gaaatatggc ctcgatgttg aactggtagg
     4081 atttagcggt tcgttgctac cgaacgatgc gacaaatcat ggcgaacttg atgcgaatgt
     4141 cttccagcat cgaccctttc ttgaacaaga taatcaggcg catggttata aactggtagc
     4201 ggtgggaaat acgtttgtct tccctatggc gggttattcc aaaaaaatca aaaccgtggc
     4261 gcaaataaaa gaaggcgcga cagtggcgat ccccaacgac ccgaccaacc ttggtcgcgc
     4321 acttttactg ttgcaaaaag agaagctaat tactctgaaa gagggaaaag ggttattacc
     4381 taccgcgctg gatataaccg ataatcctcg tcatttgcag attatggaac tcgagggggc
     4441 gcagctcccg cgcgtactgg atgatcccaa agttgatgta gcgattatca gcaccactta
     4501 cattcagcag accgggcttt ctccggtgca cgacagcgta tttattgaag ataagaattc
     4561 gccgtatgtg aatattttgg tggcacggga agataataag aatgcagaaa acgtgaagga
     4621 atttctgcaa tcttatcaat cacccgaagt cgctaaagcg gcagaaacca tttttaacgg
     4681 tggcgcggtg cctggctggt aattctcact tatgccgcgc cggacgattt atttttctgg
     4741 cgcggcgacg ggaatccatt gaaatcaaaa tcaccgatga cagaatcagc agcgttccca
     4801 gccagtccgg taatgtgaac gtgatcccca gcaacagcaa agagagtagc gcgctactta
     4861 atggttctgc acagctcaaa atgctggctt ttggaccgcc aattaattgt gctcctttca
     4921 ggtacagact aaatgtcagg gacgtaccaa tgaccaccaa ataaaaaaac gccagaatca
     4981 aactgccgtt aacgacaaag tttgttcctt gtctggcata aaaaggcaac agaatcagac
     5041 cgccaatcag catactccag ccgacgactg gtaacgtgcc atagcgggca attagcgttg
     5101 agggataggt ggtatagaat gcagcagcaa atgccgaggc aatgccccag aacaacgcgg
     5161 caggagagat cgataatgac gtcggattac cgtgtgtcac caataaaaaa gtcccgacca
     5221 gcgatgtcaa aatagcgcag aaaaccagaa tgcccgggcg cgatttacgc accagtgaga
     5281 accaggcgac gataatcgtc ggtgagagga attgcagcac cgttgccgtg gctgcgttcg
     5341 atttttcgat ggttagcaaa aaagtgagct gtacagttag cgcgccaacc acggaaaaaa
     5401 tcagcaggct aatggcatct ttatgattgt taataataga aaagatttta tcgccatgaa
     5461 caaatgacag cgtcagtaga atcaaaccgg cgaatatcaa acgcgtcata gtcaaaaact
     5521 gcgacgacat ctggctttgc tccatgatgt attgcgcgca gacccctgaa cttccccaca
     5581 acacggcggc aatcagaacg ttcagcatcc cctttctggt ggaacccatt tttccctctg
     5641 catactgttt tgtcgtttat gtatgcaggc atgatagcaa aaatggcgag gatggctctg
     5701 tttgcgccac gccgattttt cttagcccag actggtgagc taaatatctt taataaacag
     5761 caggcaaatc atcgcaatac ccaacatccc tatcgccagc cagaacaacg catggtagct
     5821 ccagatttca accataattc cgtcaacaga gccggcgata atccagccga cgcgtgaagt
     5881 atttgcatat aacgttgtcg cagagcctat tttttcaggc atcaagtcct gaaaataaag
     5941 catgccgata ccacagagaa taccaaggaa gatggcatta agaatttgca attccagctc
     6001 aaccgccgga gtcgtcgcca tgagtacgct ggcgtaaaaa cacattccac tcacgatagc
     6061 aatgagcatt aatagtcgct tgccaatacg tttcatgtaa tagcctgcga ttaacatcat
     6121 cggaatttcc agaccggcag cgataccaat catttctcca gccagtttat cggttagatg
     6181 cagttcatca ataataaata gcggcatatt tatcatgtag agattattcg ccgcccacat
     6241 cattgaacag accacaaaaa gtagccgcgt atcccgcttc ctgtgggtgg agggtaaaat
     6301 ttctacgggt tgggtaacga caggaatatt tctttgtatt gatggcaaaa acaaccagac
     6361 tatcagtccg caaacaacaa atgcgatggc agcggtgaga tacatcactt taaaactaaa
     6421 tcccattgcc agttcataag cgagcggtgg cccgataacc caggcaagcg agatctgagc
     6481 acgtaaaaat gtactgaaca tgaccgtctc acggcctgtt ctgtcggcgt gttcacgggc
     6541 gagggcgaac atttgcgggt ttgcggtgga agcaaaacta ctcagaagta cgcccgttga
     6601 gaggagaatg aagtagttgc gattccacgc aaaaagcgtg caggccagca ctccaaataa
     6661 gcaacatagc agaatcagta atttacggtc gccttgttta tcggagtgcc ttgccagaaa
     6721 ttgactgacc agaattccca taatagcgct accggtgaag aaaaaaccta ccattatagg
     6781 acgggctttc agttcatctg cgaggaatat acttagggta ggagtctgaa gagcgcccgc
     6841 aatacccgtc agaaaggcga caagtaaaaa tgccgcggca gtgagatcaa gtatttttga
     6901 tggagtggta gccgtttttt gcattcttct gaatcaccgg gcgaaaaaat ataactgagt
     6961 tacaaataag taccagtcga atgagtattg tattaagaat ttttgagtat cgctgataca
     7021 accagaaagc aatgctagcg caatattata gggccagcaa ttgagattat tagtaaaagc
     7081 atgttaagaa ttaattaccg aatttatata tta
//
LOCUS       DAZFZC010000172         7015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFZC010000172 DAZFZC010000000
VERSION     DAZFZC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.174"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            207..1082
                     /gene="rluB"
                     /locus_tag="ACHKXP_004770"
     CDS             207..1082
                     /gene="rluB"
                     /locus_tag="ACHKXP_004770"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO7649600.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1122..1712)
                     /gene="cobO"
                     /locus_tag="ACHKXP_004771"
     CDS             complement(1122..1712)
                     /gene="cobO"
                     /locus_tag="ACHKXP_004771"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO7649601.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1709..2467)
                     /locus_tag="ACHKXP_004772"
     CDS             complement(1709..2467)
                     /locus_tag="ACHKXP_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO7649602.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2687..3736
                     /gene="sohB"
                     /locus_tag="ACHKXP_004773"
     CDS             2687..3736
                     /gene="sohB"
                     /locus_tag="ACHKXP_004773"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO7649603.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3772..4023)
                     /locus_tag="ACHKXP_004774"
     CDS             complement(3772..4023)
                     /locus_tag="ACHKXP_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO7649604.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4403..7000
                     /gene="topA"
                     /locus_tag="ACHKXP_004775"
     CDS             4403..7000
                     /gene="topA"
                     /locus_tag="ACHKXP_004775"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO7649605.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1785 a         1673 c         1899 g         1658 t
ORIGIN      
        1 tgatgttaat cgaaacaagc tttaaccata aagatagtgt agtatgttgc gcctcaaagc
       61 caggccagtt aaaacgtcga gtcgtttact taaggcctga ggagttcaaa cagactttac
      121 tatcagtgag tcagatgtaa gttctgactc actgttgcac aaaagcatat cagacgcctg
      181 ggaaggcgac accaaaagga agctctatga gcgaaaagct acagaaagtg ctggcgcgtg
      241 ccggccacgg ttctcgccgt gaaatcgaat ctattattga agccggtcgt gtgagtgttg
      301 atggcaaaat tgctaaactc ggcgatcgcg ttgaagttac ccctggattg aaaatccgta
      361 tcgatggtca ccttatttcg gtacgtgagt ccgctgaaca aatttgtcgc gttctggctt
      421 attacaaacc ggaaggtgag ttgtgtaccc gtaacgatcc ggaagggcgt ccgacggtgt
      481 ttgaccgtct gccaaagctg cgtggcgcac gctggattgc cgtaggtcgt ctggacgtta
      541 acacctgtgg tctgctgctg ttcaccactg acggtgaact ggcaaaccgt ttaatgcatc
      601 caagccgtga agttgaacgt gaatacgccg tgcgtgtatt tggtcaggtt gacgacgcga
      661 aactgcgtga tttgagtcgt ggcgtgcaac tggaagatgg tccggcagct tttaaaacca
      721 tcaagttcag cggcggcgaa gggatcaacc agtggtacaa cgttactctg accgaaggcc
      781 gtaaccgtga agttcgtcgt ctgtgggaag cggttggtgt gcaggtcagc cgcctgatcc
      841 gtgttcgtta cggtgatatc ccactgccaa aaggtctgcc acgcggtggc tggacggagc
      901 tggacctcgc ccagactaac tatctgcgcg aactggtgga gctaccgcca gaaaccagct
      961 ctaaagtcgc tgtagaaaaa gaccgtcgtc gcatgaaggc gaatcagatt cgtcgtgcgg
     1021 tgaaacgtca cagtcaggtg agcggcggtc gtcgttctgg cggacgtaat aataacggtt
     1081 aatcaaagta tgccggacgt catatccggc atttttacag attaataatc gatccctatc
     1141 tgcgctttta caccggcatc aaacgcatgt ttgacggggc gtaattcact taccgtatct
     1201 gccagttcaa gaatatcccg atgacaacca cgacccgtga taatcaccgt ctgttgatgt
     1261 ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata cgccaccata
     1321 tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat ccgctttgca
     1381 tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt atcccaggta
     1441 aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt gcgttcgcca
     1501 ttaggccagg tgcctttaat aaactgcacg acgcctactt tttttccgtg accaactgcg
     1561 cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt aaagacgatg
     1621 ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc tttcactcgc
     1681 tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta cggcccggtt
     1741 gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc cagaggtaga
     1801 gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg aaggcgctgg
     1861 cacgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg cgctgctgat
     1921 attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct gcatatgcgc
     1981 cccagttggc tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt gaaccggcgt
     2041 ccgatttgag taataaagga agcagtgcct gggtgagcat aaaggtggca ttaacgttga
     2101 cctgtatgac gtcctgccag acctgtggat tttgttcgct cattggacaa acatcgccga
     2161 gcaatccggc attatgcaaa acaccatcca gacgcggata attaacggca atgcgctgtg
     2221 ccagttgttg gcaatcttcg gacgtgcagg tcagcaaatc gagaataaac cactgtggct
     2281 gacgcccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct tcattacggc
     2341 ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca cgaccaatac
     2401 catcactggc tcccgtcacc aggataatgc gatcattgag taaatcttgt tttggctggt
     2461 aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact ttatgacttt
     2521 ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata acaccgcaaa
     2581 atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct taacatgcct
     2641 gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg aattgttgtc
     2701 tgaatatggt ttgtttttgg cgaaaatcgt taccgttgtg ctagcgattg cggcgattgc
     2761 cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt tacgggtcaa
     2821 caatctcagc gaacagtata aggagatgaa agaagaactg gccgcggcgc tgatggactc
     2881 acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cataagcaag aagcgaaagc
     2941 agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacccc gcgtctgggt
     3001 gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg aagagataac
     3061 ggctgtactc gcagcattca aaccgcagga tcaggttgtg ctacgtctgg aaagccctgg
     3121 tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc gtgataaaaa
     3181 cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca tgatggcctg
     3241 tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg gcgtggtggc
     3301 gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg aactgcacac
     3361 cgccgggcag tataagcgca cgctgacctt gctgggtgaa aacaccgaag aagggcggga
     3421 gaaattccgc gaagagctga acgaaacgca tcagttattt aaagattttg tgaagcgtat
     3481 gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg gacaacaggc
     3541 ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc ttagcctgat
     3601 ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca ttgaccgatt
     3661 caccggcagc gcggcagaga gcgccgatcg attgttgtta cgctggtggc agcggggtca
     3721 aaagccattg atgtaaaaga caaacgcgag ggtaagacct cgcgttttgc tttaatcaac
     3781 caggtgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa acaccgcggt
     3841 gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa ataccagcac
     3901 gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct catgctcacg
     3961 aatgattttg ttggcctctt tcaacagcgt ttcgcggtcg atgggttgtg tctctttgtt
     4021 cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg cagacaaata
     4081 taccacgctg gtggcaagag cgccttactg gcaactttgg attttgcatg ctaataaagt
     4141 tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca atagatttgc
     4201 ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc
     4261 agtcacctga attttcgtga acagagtcac gacaaggggt tgatatccgc agagagcgag
     4321 tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca ttcctggaag
     4381 aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt ccccggcaaa
     4441 agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca gcgtcggtca
     4501 catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact ctacctccac
     4561 caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt
     4621 tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt
     4681 ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga
     4741 ccgcgaaggg gaagccattg catggcacct gcgggaagtg attggtggtg atgatgcgcg
     4801 ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg catttaacaa
     4861 accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct ttatggaccg
     4921 cgtggtgggg tatatggttt cgccgctgct atggaaaaag atcgctcgtg gtctgtctgc
     4981 cggtcgtgtg cagtcggtgg cggttcgcct ggtggtcgag cgtgagcgtg aaattaaagc
     5041 gttcgtgccg gaagagttct gggaagtcga tgccagcacg accacgccat ctggtgaagc
     5101 gttggcgttg caggtgactc atcagaacga caaaccgttc cgtccggtca acaaagaaca
     5161 aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg aacgtgaaga
     5221 caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc aacaagctgc
     5281 cagcacccgt cttggatttg gcgtgaaaaa aaccatgatg atggcgcagc gtttgtatga
     5341 agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg acgcggtaaa
     5401 tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg aaagtccgaa
     5461 tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc cttctgacgt
     5521 caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt
     5581 aatctggcgt cagttcgttg cctgccagat gaccccagcg aaatatgact ccacgacgct
     5641 gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc gctttgatgg
     5701 ctggacgaaa gtgatgcctg cactgcgtaa aggcgatgaa gatcgtatct taccggcagt
     5761 cgataaaggc gatgctctga cgctcgttga actgacacca gcccagcact ttaccaagcc
     5821 gccagcccgt ttcagtgaag catcgctggt taaagaactg gaaaaacgtg gtatcggtcg
     5881 tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctatg tgcgagtaga
     5941 aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc tggaagagaa
     6001 tttccgcgag ttaatgaact acgacttcac cgcgcagatg gaaaacagcc tcgaccaggt
     6061 ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg atttcaccca
     6121 gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga accagatggt
     6181 tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca cagcgagcac
     6241 cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt gcaaaaccac
     6301 cattaacctg gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag acgctgaaac
     6361 caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg acagctatct
     6421 catcgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg acggttacga
     6481 gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa
     6541 atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg cctgcaccaa
     6601 cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac caccgaaaga
     6661 agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt tcgtgctgcg
     6721 tgacggcgct gccggtgtgt tcctggctgc caacactttc ccgaaatcgc gtgaaacgcg
     6781 tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa aactgcgtta
     6841 tctggcagat gcgccacagc aggatcctga aggtaataag accatggttc gctttagccg
     6901 taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg gctggtcagc
     6961 attttatgtt gatggcaaat gggttgaagg gaaaaaataa cctttaattc tgtca
//
LOCUS       DAZFZC010000173         6964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFZC010000173 DAZFZC010000000
VERSION     DAZFZC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.175"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1204
                     /locus_tag="ACHKXP_004776"
     CDS             <1..1204
                     /locus_tag="ACHKXP_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhiJ/YhiL family protein"
                     /protein_id="HFO7649606.1"
                     /translation="NDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQFADRIY
                     FSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIEDEVL
                     NVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRF
                     SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKD
                     YPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHH
                     AIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRE
                     RIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLI
                     RRRTMLQL"
     gene            1394..1507
                     /gene="yhiY"
                     /locus_tag="ACHKXP_004777"
     CDS             1394..1507
                     /gene="yhiY"
                     /locus_tag="ACHKXP_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO7649607.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1570..2637
                     /gene="yhiI"
                     /locus_tag="ACHKXP_004778"
     CDS             1570..2637
                     /gene="yhiI"
                     /locus_tag="ACHKXP_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFO7649608.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2634..5369
                     /gene="rbbA"
                     /locus_tag="ACHKXP_004779"
     CDS             2634..5369
                     /gene="rbbA"
                     /locus_tag="ACHKXP_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO7649609.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGLGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5369..6493
                     /gene="yhhJ"
                     /locus_tag="ACHKXP_004780"
     CDS             5369..6493
                     /gene="yhhJ"
                     /locus_tag="ACHKXP_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7649610.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(6658..>6964)
                     /locus_tag="ACHKXP_004781"
     CDS             complement(6658..>6964)
                     /locus_tag="ACHKXP_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7649611.1"
                     /translation="EMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDD
                     CKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1645 a         1734 c         1893 g         1692 t
ORIGIN      
        1 aaatgataat gctaatgatg ttgcagttac tgagtcgtac aaacccatct ggaactggaa
       61 aatatcgcaa tataatgtat ctgatattaa attcgaaaca atgataaaac cccagttcgc
      121 tgacagaatc tatttctcca attgtttacc cgttgatccc acaagtacca gaccaaccta
      181 ttttggtgat accgatggct cagtcggtgc agttttgttc gcgcttttcg caacgggaca
      241 tttgggaatc atggctgaag gagaaaattt ccttagccag ttgcttaata tagaagatga
      301 agttctcaac gtattgctaa gggagaactt taatgagcaa cttaacacta atgttaatac
      361 aataatcagt atcctgaaca gaagagacat tattttagaa agtcttcagc cttatttagt
      421 tattaataaa gacgctgtga cgccatgtac gtttctgggc gaccaaaccg gcgatcgatt
      481 tagcaatatc tgtggtgatc aatttatcat cgatttgtta aaacgcataa tgagtatcaa
      541 tgaaaatgtt catgtattag ctggaaatca tgaaacgaat tgcaatggta actatatgca
      601 gaacttcacg cgtatgaaac cactcgatga ggatacctac tctgggataa aagattatcc
      661 tgtatgtttc tatgacccca aatacaaaat aatggcaaat catcatggga taacttttga
      721 tgatcagcga aaacgttaca ttataggacc gataacagtc tctattgatg aaatgaccaa
      781 tgctctcgat ccggtcgaat tagcggcaat tatcaataaa aagcatcatg ctataattaa
      841 tggcaaaaag tttaaaacta gccgggctat ctcttgtcga tcctttaatc gttatttttc
      901 tgtttcgaca gattacagac ctaaacttga agcactatta gcatgttcac aaatgttagg
      961 tataaatcaa gttgttgcac ataacggaaa tggtggaaga gaacgcatag gcgaaacggg
     1021 gacagtttta ggtcttaacg cccgtgacag taaacatgct ggaagaatgt tcagcatgca
     1081 taattgccaa attaaccctg gtgcagggcc tgagataacc actccctgga agtcttacca
     1141 gcatgaaaaa aacagaaatg gacttatgcc tttgattaga aggcgcacaa tgttacagct
     1201 ctgaaccata gacaacatga atattaatgt tactatctgg ttgagactgt tgataaaacg
     1261 taaaaaggaa tgctctgttc ctgcaactga acaggcgtga aacgctatcc aacaggatgg
     1321 ataccgtttt gtcagccagt tttatagatc acacttatga gtatgcgtgt ttcatttacg
     1381 gccaggaatg ctgatgatga caacattact acccgttttc actaagcctt ccccgttggc
     1441 gctcaatgct ctgcgcgctg gtcgtatttg ccgtttcctt cttatcccgg atggaagaat
     1501 ccgctgaacg tcgttcggcg gcagttgtaa cacgcgtcgt ttgcttacag ggattatggc
     1561 gggtcgatta tggataagag taagcgccat ctggcgtggt gggttgtcgg gttactggcg
     1621 gtggcggcta tcgtggcgtg gtggctgttg cgcccggcag gtgtgccgga aggctttgct
     1681 gtcagtaatg ggcgcattga agcgacggaa gtggatattg ccagcaaaat tgccgggcgt
     1741 atcgacacca ttctggtgaa agaaggccag tttgttcgcg aaggtgaagt gctggcgaag
     1801 atggatactc gcgtgttgca ggaacagcga ctggaagcca tcgcgcaaat caaagaggca
     1861 caaagcgccg ttgctgccgc gcaggctttg ctggagcaac gacaaagcga aactcgtgcc
     1921 gcacagtcgc tggttaatca acgccaggca gaactggact ccgtagcaaa acgtcatacg
     1981 cgttcccgtt cactggccca acgaggggct atttctgcgc aacagctgga tgacgatcgc
     2041 gccgccgctg agagcgcccg agctgcgctg gaatcggcga aagctcaggt atcggcttct
     2101 aaagcggcta tagaagcggc acgcaccaat atcattcagg cgcaaacccg cgtcgaagcg
     2161 gcacaagcca ctgaacggcg cattgccgca gatatcgatg acagcgaact gaaagccccg
     2221 cgtgacggac gcgtgcagta tcgggttgcc gagccaggcg aagtgctggc ggcaggcggt
     2281 cgggtgctga atatggtcga tctcagcgac gtctatatga ctttcttcct gccaaccgaa
     2341 caggcgggca cgctgaaact gggcggtgaa gcccggctga tcctcgatgc cgcgccagat
     2401 ctgcgtattc ctgcaaccat cagttttgtc gccagtgtcg cccagttcac gccaaaaacc
     2461 gtcgaaacca gcgatgaacg gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg
     2521 gaattactcc agcagcatct ggaatatgtc aaaaccggtt tgccgggcgt agcgtgggtg
     2581 cgggtgaatg aagaacttcc gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc
     2641 atctggaact ggttcccgtc ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg
     2701 gaaaaaccgt tgcgctgaac aatatcactc tcgatattcc ggcccgctgt atggtcgggc
     2761 tgattggccc ggacggcgtc gggaagtcga gcttgttgtc gttgatttcc ggtgcccgcg
     2821 tcattgaaca gggcaatgtg atggtgctgg gcggcgatat gcgcgacccg aagcatcgcc
     2881 gcgacgtctg cccgcgcatc gcctggatgc cgcaggggct gggcaaaaac ctctaccaca
     2941 ccttgtcggt gtatgaaaac gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg
     3001 agcgggaagt gcgaatcaat gagctgctga ccagcaccgg gttagcaccg tttcgcgatc
     3061 gtccggcagg gaaactctcc ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa
     3121 tccacgaccc ggaactgttg atccttgatg agccaacaac gggggttgac ccgctctccc
     3181 gctcccagtt ctgggatctg atcgacagta ttcgccagcg gcagagcaat atgagcgtgc
     3241 tggtcgccac cgcctatatg gaagaggccg aacgcttcga ctggctggta gcgatgaatg
     3301 ccggagaagt gctggcaact ggcagcgccg aagagctacg gcagcaaacg caaagcgcta
     3361 cgctggaaga agcatttata aatctgttac cgcaagcgca acgccaggcg catcaggcgg
     3421 tagtgatccc accgtatcaa cctgaaaacg cagagattgc catcgaagcg cgcgatctga
     3481 ccatgcgttt tggttccttc gttgccgttg atcacgttaa tttccgcatt ccacgcgggg
     3541 agatttttgg ttttcttggt tcgaacggct gcggtaaatc caccaccatg aaaatgctca
     3601 ccggactgct gcccgccagc gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa
     3661 aagatatcga tacccgccgt cgggtgggct atatgtcgca ggcgttttcg ctctataacg
     3721 aactcaccgt gcggcaaaac cttgagttac atgcccgttt gtttcacatc ccggaagcgg
     3781 aaattcccgc aagagtggct gaaatgagcg agcgttttaa gctcaacgac gttgaagata
     3841 ttctgccgga gtcattgccg ctcggcattc gccagcggct ttcgctggcg gtggcggtga
     3901 ttcatcgccc ggagatgtta atcctcgatg agcctacttc tggtgtcgat ccggtggcga
     3961 gggatatgtt ctggcagttg atggtcgatc tctcgcgcca ggacaaagtg actatcttca
     4021 tctccaccca ctttatgaac gaagcggaac gttgcgaccg catctcactg atgcacgccg
     4081 gaaaagtgct tgccagcggt acaccgcagg aactggttga gaaacgcgga gccgccagtc
     4141 tggaagaggc atttatcgcc tatttgcagg aagcggcagg gcagagcaac gaagccgaag
     4201 cgccgcccgt ggtacacgac accacccacg cgccgcgtca gggatttagc ctgcgccgtc
     4261 tgtttagcta cagccgccgc gaagcgctgg aactgcgacg cgatccagta cgttcgacgc
     4321 tggcgctgat gggaacggtg atcctgatgc tgataatggg ttacggcatc agtatggatg
     4381 tggaaaacct gcgctttgcg gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga
     4441 cactcaacct ctccggttcc cgttacttta tcgaacagcc gccgctcacc agttatgacg
     4501 agcttgatcg tcggatgcgt gcgggcgata tcacggtggc gattgagatc ccgcccaatt
     4561 tcgggcgcga tatcgcgcgt ggtacgcctg tggaactcgg cgtctggatc gacggagcga
     4621 tgccgagccg tgctgaaacg gtaaaaggtt acgtgcaggc catgcaccag agctggttac
     4681 aggatgtggc gagccgacaa tcgacacccg ccagccaaag cgggctgatg aatattgaga
     4741 cgcgctatcg ctataacccg gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc
     4801 cgcttctgct gatgatgatc ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag
     4861 agcttgggtc gattatcaac ctttacgtga cccccaccac gcgtagtgaa tttttgcttg
     4921 gtaaacagtt gccatacatc gcgctgggga tgctgaactt tttcctgctc tgcggcctgt
     4981 cggtgtttgt gtttggcgta ccgcataaag gcagtttcct gacgctcacc ctggcggcgc
     5041 tgctgtatat catcattgcc accggaatgg ggctgctgat ctccaccttt atgaaaagcc
     5101 agattgccgc cattttcgga acggcgatta tcacgttgat cccggcgaca cagttttccg
     5161 ggatgatcga tccggtagct tcgctggaag ggcttggacg ttggatcggc gaggtttacc
     5221 cgaccagtca ttttctgact atcgcccgcg ggacgttctc gaaagcgctg gatctgactg
     5281 atttgtggca actttttatc ccgttgctga tagccatccc gctggtgatg ggcttaagta
     5341 tcctgctgct gaaaaaacag gagggatgat gcgccattta cgcaatattt ttaatctggg
     5401 tatcaaagag ttgcgcagtc tgctcggtga taaagcgatg ctgacgctga ttgtcttctc
     5461 gtttacggtg tcggtgtatt cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc
     5521 gatcgccatt gccgatatgg atcaatcgca gttatcgaac cggatcgtta acagcttcta
     5581 tcgtccgtgg tttttgccac cggagatgat caccgccgat gagatggatg ccggactgga
     5641 cgccggacgc tataccttcg cgataaatat tccgcctaat tttcagcgtg atgtcctcgc
     5701 cggacgccag ccggatattc aggtgaacgt cgatgccacg cgcatgagcc aggcatttac
     5761 cggcaatggg tatatccaga atattatcaa cggtgaagtg aacagctttg tcgcgcgcta
     5821 ccgtgataac agcgaaccgt tggtatcgct ggaaacccgg atgcgcttta acccgaacct
     5881 cgatcccgcg tggtttggcg gggtgatggc gatcatcaac aacattacca tgctggcgat
     5941 tgtattgacc ggatcggcgc tgatccgcga gcgtgaacac ggcacggtgg aacacttact
     6001 ggtgatgccg ataacgccgt ttgagatcat gatggcgaag atctggtcga tggggctggt
     6061 ggtgctggtg gtatcgggat tatcgctggt gctgatggtg aaaggtgtac tgggcgtacc
     6121 gattgaaggc tcgatcccgc tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac
     6181 gtcaatcggc atttttatgg ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt
     6241 gattctggtg ctgctgccgc tgcaaatgct ttccggtggt tccacgccgc gcgaaagtat
     6301 gccgcagatg gtgcaggaca ttatgctgac catgccgacg acacactttg ttagcctcgc
     6361 gcaggccatc ctctaccggg gtgccggatt cgaaatcgtc tggccgcagt ttctgacgct
     6421 gatggcaatt ggcggcgcat ttttcaccat tgcgctgctg cgattcagga agacgattgg
     6481 gacaatggcg taaatacaat tgcccgatgc gtaagcttat agggactacg catcggtgca
     6541 atttattgaa ttttagttgt ttttaggccg gataaggcgt ttatgaactg acccccaaaa
     6601 cctggacagt ttcagttaac cggctttttg atactcagtc ctgtattcag ggcaagatta
     6661 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     6721 catcttacgt cttaaccctg ccttgaatac cttatcattc gtcaaaatat taatagcaat
     6781 gtgccgtatc cctgaaaata attctgctgc atttcctctt cttattttgc agtcgtcttc
     6841 attcattacc acgtccagac gccagtgcag cttattctcc acgtgccagt gatttcggat
     6901 cgctgtggcg aatttctcag cggttaaatc agcagaactg atataatatc tgaccgtcat
     6961 ttct
//
LOCUS       DAZFZC010000174         6896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFZC010000174 DAZFZC010000000
VERSION     DAZFZC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.176"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..6043
                     /gene="efa1"
                     /locus_tag="ACHKXP_004782"
     CDS             <1..6043
                     /gene="efa1"
                     /locus_tag="ACHKXP_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO7649612.1"
                     /translation="YNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELPPHELLT
                     PVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMREG
                     TLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADATL
                     SLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVASSL
                     HFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALL
                     YLQTKDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNK
                     YITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFGHA
                     DFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPENDAG
                     LSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSLRL
                     NGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQ
                     RQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGETLH
                     TTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTLAK
                     INLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTMGK
                     ALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLTAA
                     SVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVASPE
                     RGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYANS
                     ISPYSSLAHGYANSKWPRTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWREAV
                     PDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFILR
                     GGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLTFDLSKQTQSVMLKTPDD
                     EVPVMTITQKGINTLVGTAAGKDRLIGNDKDNTFHTSSGGGTVISGGGNNRYIIPRDL
                     KTPLTLTLSSNSVSHEIFLPETTLAELKPVAFELSLIYWAGNNINVQPEDEAKLNHFA
                     GNFRVHTRDGMTLEAVSRENGIQLAISLCDVQRWQAVYPEENNRPDAILDRLHDMGWS
                     LTPEVRFQGGETQVSYDPLTRQLVYQLQARYSEFQLAGSRHHTTAVTGTPGSRYIIMK
                     PVTTQILPTQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLTIS
                     GIEESLPETTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMALNQ
                     VMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDLSI
                     PPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEAPS
                     LILSEFRRFTISSDKTFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFRDF
                     FTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYARI
                     QKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGELFPTFHPWYTDDLSGRY
                     KSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILSEI
                     PQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHSSG
                     RVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRSTV
                     SKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIADN
                     APGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6215..6565
                     /locus_tag="ACHKXP_004783"
                     /pseudo
     CDS             6215..6565
                     /locus_tag="ACHKXP_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6697..>6896
                     /locus_tag="ACHKXP_004784"
     CDS             6697..>6896
                     /locus_tag="ACHKXP_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7649613.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGHVSRRLPVT"
BASE COUNT         2057 a         1560 c         1576 g         1703 t
ORIGIN      
        1 ttataatgaa gatgaaatta gtgcgaaatt accactagca tatgattaca ttttaaataa
       61 aaaagaatca agctctattc ctgttgaaat tttgaacaaa ctttcagagt taccaccaca
      121 tgaactactc acacctgttc ttggccagag tgttaatcct ctgggcatgg gctactcatc
      181 cgataatgga aaaatcacag agcaagtaat agtcagtgga gctgatggat ttgataatcc
      241 catatctgga cttatatata cctatcttga agatctatat aacatccatg taaggatgcg
      301 agaaggtaca ctaaattcac agaatcttcg tcagcttctg gaaaactctg tttcttcatg
      361 ctttttgact gagcaaagta ttaataaatt acttagtgag gcagaaaaaa ggccttatca
      421 gtctttaaca gaaatacatc agcatctcac aggattacca actattgccg atgcaaccct
      481 ttcattactt tctgttggat tacctggtac gggaaaacta ttgcgcaggg agcaggacta
      541 tgggcgtcca ccagttacag caattcagga ttccacattt gtactccctt ataatttcaa
      601 aggtattggt tttaacgata acattatatc ctctgcacct gtagcctcct cattacattt
      661 tatcgctgaa catgcgaaat atactttatt gtcatggcct gagttttatc gtcatcatgc
      721 acagcgatgg ttcgaaatgg ctaaaggata tggaagccag aatattgatt ttcaccctca
      781 gtctctattg gtaacccaag aaggacgctg tatgggatta gccttacttt atttacagac
      841 taaagatact gctcattata gcattctcca ggaaaaccta atgactgtga gtgcacttca
      901 tcagaccagt aatcgcgata agttgccact gtccaaagat gataattcct taatgacaag
      961 aacttatagt ctgattgaaa tgctacagta tcagggaaac aaatatatta ccaacgaatc
     1021 gctactacat aagaccgcat ggaaccaaga aagaataact ttattattca atgaaaaagg
     1081 agttaagcga gccctgataa gcacgcctaa tcatactctg gttctgcaac aactggagga
     1141 tatttaccgg ctcactgatc caaattttgg gcatgcagat ttcctttcac ctatagatgc
     1201 tctgaagttt attgaggcta tgatacaatt aactccaaca cttcaggaat attatggcct
     1261 attaaacaaa gacattaata aacatataca agtacattat gccgaatcag atatggtctg
     1321 gaataagctt ctgccagaaa atgatgctgg actgagcacc agaattcagc acaccaccac
     1381 cgaccgtctg gcgaatctgg ctgaaccagt cgctgttgca ggtatctccc tgccagtaaa
     1441 aacactttat gatatcggag ccacccttga cggtcggcgc atcacctctc ctccaacatc
     1501 ggagcaaatc ccttctctgc gtctcaacgg tgatgttctg aatgattatc tgtcccgcac
     1561 agttctgact ccagaacagg ctgataacat aagaaaaata ctgcacactc agggaatacg
     1621 cagcggtacc cgtcccatag atccggagat gattcgtggg acgcaggatg acctagtttc
     1681 gtcacagact cgtctgcaaa ggcaagcaac acgggttaaa cagcaactcg ccggtgtact
     1741 tgatactctg caacagcact tccagaacat tccacgttca tccggtcgtc atctttctgt
     1801 agagaatatt gagctggctg atatcggaag tgggcgtttc aaccttcaaa ttcgagatgg
     1861 agaaacattg cataccactt ctgtggaagt accggaagtt gtgtcccgtt ttcagaaact
     1921 ttctaccatg ctttcagccc tgcctgccag tggaatcatg gatttcgacc tcggcatgag
     1981 tgtggtcgga gtcgtccagt atgcccgcct gctacagcag gggcacgaag acagtaccct
     2041 agccaagata aatctagcta tggatatcaa gcaactttcc gaagcaactc tcggcagcat
     2101 gattcagatt gccgggaata agtttctcaa tacagaagga atccaggggt tcagactgga
     2161 aagcgccgtc gctgaaggta tgcgctcagt agcaacccgt accggaggca caatggggaa
     2221 agccctttct gccagtgccc gtgttcttga actgcctgta ctggaaacag ttctggggac
     2281 atggaacctg tacaacagcg tcattcagct ccagcaagct acatcttatt ctgagacaat
     2341 ggctgcccgg gtacagattg cgtttgattc catttctctg ggattaactg ccgcttcggt
     2401 agctttcccg ccactaatta ttgccactgg ccccattgcg gctattggta tgggagcttc
     2461 cagtattgca cgtaatgtgg cacggaaaga agaacggcat acacaatggc tggaatataa
     2521 aaaattcctg actgatggca gtaaacacat tgttgtggcc tctccggaaa gaggtctgct
     2581 ggatttctcc ggaaacaaag tttttggaaa aatggtgctg gatctgcgtc agtctcctcc
     2641 tctcttgcat ggagaaagct cttttaacgc tgaccgcaaa atcggtcatc gtccggatct
     2701 gggggactgg caaattcgtg agaaggtggg gtatgccaac agtatcagtc cctactcttc
     2761 tctggcgcac ggttacgcca acagtaaatg gccacgaaca ataccgaaaa ttccctcggg
     2821 agaatatgac accataattc tgggctacgg tcaccagtat caggccaata cggaaataga
     2881 atatctgtct aactggattg tatggcggga agccgtacca gacagtactt cccgccacaa
     2941 acgtcctcct ctggaggttc ttaatagtca gtgtactgtg atagctggag agcgtaaaac
     3001 cacagtactt cccctgagag tgctcagcga tctgacaccg gaatgcacag aacaggctat
     3061 atcgttaaaa gattataaat tcatactgag agggggaagc ggtgggctgg ctgttcaggt
     3121 cggtggcgcg ggatattatg atattgatgc aaatcctgtg gcaaaagaaa atacgctctc
     3181 ttttcgcggg ctaccggaag agtttccgct cacctttgat ttatcaaaac aaacacagtc
     3241 ggtcatgctg aaaacaccag acgatgaggt gccggtaatg accattaccc agaagggaat
     3301 aaacaccctg gtaggtacag ccgccggtaa agaccgacta atcggtaacg ataaggacaa
     3361 taccttccat acaagctctg gcggcggtac agtcatctcc ggaggcggga ataaccgcta
     3421 tattatcccc cgggatttaa aaacgccgtt gacactgacg ctgtccagta actcagtctc
     3481 tcacgaaatc tttctgccag aaacaaccct agctgaatta aaacctgtcg cctttgagct
     3541 gagtttgatt tactgggccg ggaacaacat aaatgttcaa ccagaggatg aagcaaaact
     3601 gaaccacttt gccggaaact tcagggtgca tacccgtgat ggcatgactc tggaggcggt
     3661 ttcccgggaa aatggtattc aactggcgat ttcattatgt gatgttcaac gctggcaggc
     3721 tgtttatccg gaagaaaata acagaccgga tgccatactg gacaggctgc atgatatggg
     3781 ctggagtctg acaccagaag tccggttcca aggaggagaa acacaagtca gctatgatcc
     3841 cctgactcgt cagctcgttt accagcttca ggcgcgttac tctgaattcc agttggccgg
     3901 tagtcgccac cataccacgg ctgtaaccgg aactccggga agccgataca ttatcatgaa
     3961 gccagttaca acacagatat taccgacaca aatcatactg gctggtgata atgaccatcc
     4021 ggaaacgatt gatttactgg aagctagtcc tgttctggtt gaagggaaaa aagacaaaaa
     4081 cagcgtgata ttaacgattg ctacgattca gtattccctt caactgacaa tatccgggat
     4141 cgaagaatcg ctgcccgaga caacccgtgt ggcaattcag cctcaggata cccgtttact
     4201 gggtgacgta ctccggatct taccagataa tggtaactgg gtggggattt tccggagtgg
     4261 tcatacacca acggtaaacc ggctggaaaa tttgatggca ctgaatcagg taatgacgtt
     4321 cctgccccgg gtatccggaa gtgcagagca ggtattatgc ctcgaaaacc taggtggcgt
     4381 aaggaaaaaa gtggaggggg agttactgtc agggaagctg aaaggtgcgt ggaaagccga
     4441 aggtgaacct actgttccgg taaatatctc agatctaagt atcccaccct attcacgtct
     4501 gtatctgatt tttgaaggga aaaataatgt gttgctacgc agtaaagtac atgcagctcc
     4561 gttgaaaata acatccgcag gagagatgca gttatctgaa aggcagtggc aacagcagga
     4621 acatattatt gtcaagcccg acaacgaagc cccctcatta atactcagtg aatttcgtcg
     4681 tttcactatt tcatcggata aaacattttc tttaaaactg atgtgccatc agggtatggt
     4741 ccgcatcgac cgcagatcat tatcggtcag attgttctat ctgcgtgaac agccaggtat
     4801 cggcagttta cgtctgacgt tcagagattt tttcacagaa gtgatggata caacggacag
     4861 ggaaattctg gagaaagagc tgagaccaat tctgatagga gatacacacc gctttatcaa
     4921 cgctgcatac aaaaatcatc tgaatatcca gttaggagat ggcgttctga atctggcaga
     4981 cattgttgcg gaatacgccc gtattcaaaa ggaagaaaca tcaaaaatat tgtatcaata
     5041 tcaaggtgcc atgaaaaaaa aaacagatgg accatctgtg gtagaagatg ccattatgac
     5101 cactactgtc acaacagatt caggtgaact attccctacc ttccacccgt ggtatacaga
     5161 tgatttatca gggcgttata agagcgtacc tatggcaaga aaagcagata ctttgtatca
     5221 cctgacacca aaaggtgatc tacagataat atatcaggta gctacaaaaa tggtgaatca
     5281 ggcgatgatt gtatccctgc caaactaccg acacgagtgg gaaaaatata atttaagcat
     5341 cttatccgaa atccctcaga acaataatac tgttgtacat tcaatcctca gggttaatgg
     5401 ccccacaatg caggtgcgca caattgacta cagaggaacg gatgaaaaca atcccatagt
     5461 atctttttca gatacaacct tcatcaatgg tgaacagatg ttgagttatg actcgcattc
     5521 atcagggcga gtctattcca gagaagaata tatgatgtgg gaattgcagc aacgggtatc
     5581 agaagcttcc agtgcccgga cacaggatta ctggctgatg gatgcagcgg taagaaacgg
     5641 agaatggaag atcacaccag aattattacg tcacacaccg ggatatatcc ggagtacggt
     5701 atcgaaatgg tccagaggat ggctgaaaac cggcacaata ctccagactc cagaagacag
     5761 aaatacggat gtatacctga ctaccataca gaacaatgta tttagtcgtc aggggggcgg
     5821 ctaccaagtg tattatcgga ttgatggaat ggctggtgcg gatatagcgg ataatgcacc
     5881 aggggaaacc cgctgcaccc tcaggcccgg aacatgtttt gaagtgacaa gtgtggatga
     5941 aaggcattat gagtggaata tcatttatgt cacgctgaaa acctgtggct ggagccgaaa
     6001 tggccaaagc aaaacgccga atggtgacaa cctttttaac taattaaaat ctctgttaaa
     6061 gatgtataac ccggcatcta tgtgctcacc agcatataga tgcaatgtga ttcccctgat
     6121 ggtcgtatca gagatggtat gcgagcctgt acatagattt gtgtaattgc ctgattttga
     6181 tatgttcaat ccaacatcaa aagcaggtta atttatggac gaaaaacagt tgcaggctct
     6241 ggctaacgaa ctggccaaaa atctcaaaac ccctgaagat ctcagccagt tcgatcgcct
     6301 gctgaggaaa atcagcgttg aggcagctct caacgctgaa atgtcccacc atctgggcta
     6361 cgataaaaac cagcctaaac cgggtgccaa ctcccgcaat ggctattcca caaagaccgt
     6421 tatcatcggt gatggccccc tggaactacg cactccgcgc gatcgtgatg gctcttttga
     6481 accgcaactt gtgaagaaaa accagacccg gatcaccggg atggataacc agatcctgtc
     6541 gttgtacgcc aaaggcctga caaccgtaag cgtacagcct gaaccgtctg gtcagaatct
     6601 gacgaattag agtaagcgta aaccgaccgc cgtatgtagc cattagacaa gaattggtaa
     6661 cttagacgcc catctgacac agacggacat ctaagtatgg aattacagga ctggcgaaaa
     6721 gaacctcgta aaaagtattc gaatgaattc aaacttcgta tggttgaact ggcatcacaa
     6781 cctggagctt gtgttgcaca gattgcacga gaaaatggcg tcaatgataa tgttattttc
     6841 aaatggctca ggctctggca gaacgaaggg catgtttcgc ggcgtcttcc ggtaac
//
LOCUS       DAZFZC010000175         6678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFZC010000175 DAZFZC010000000
VERSION     DAZFZC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.177"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            573..1847
                     /locus_tag="ACHKXP_004785"
     CDS             573..1847
                     /locus_tag="ACHKXP_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO7649614.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVNEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(1989..3113)
                     /locus_tag="ACHKXP_004786"
     CDS             complement(1989..3113)
                     /locus_tag="ACHKXP_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO7649615.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(3843..4040)
                     /locus_tag="ACHKXP_004787"
     CDS             complement(3843..4040)
                     /locus_tag="ACHKXP_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7649616.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(4106..4376)
                     /locus_tag="ACHKXP_004788"
                     /pseudo
     CDS             complement(4106..4376)
                     /locus_tag="ACHKXP_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4610..4882)
                     /locus_tag="ACHKXP_004789"
     CDS             complement(4610..4882)
                     /locus_tag="ACHKXP_004789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649617.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            5202..5396
                     /locus_tag="ACHKXP_004790"
     CDS             5202..5396
                     /locus_tag="ACHKXP_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649618.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            5757..5885
                     /locus_tag="ACHKXP_004791"
     CDS             5757..5885
                     /locus_tag="ACHKXP_004791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649619.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(6195..6531)
                     /locus_tag="ACHKXP_004792"
                     /pseudo
     CDS             complement(6195..6531)
                     /locus_tag="ACHKXP_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2024 a         1477 c         1353 g         1824 t
ORIGIN      
        1 ttgcaacacc aaattaatat ctgaacataa atgagcagga ggtttttaaa atgagagaaa
       61 tatcagatca catgcttgat ttcgtgaaaa gagggatgaa tcttaatgga ttacctagtt
      121 ctactaatgt aatagatcta cgaggaaaag atatgggaac atatattgat gctaatggag
      181 catgctgggc tccgggtact ccatccatca tcatgtatcc ggggggaagt ggaccttctt
      241 atagtatgag tagttccaca tccagtgcaa acagcggcag ttaaataaat accctccggc
      301 atggccggag ggtatttatg atgtgcctgt aagactctac ttctgtcgtc tctttacaaa
      361 ggcatatgat ctgtcatttt gtaccagaca tcttaacgag agccttttca tatattactt
      421 acatacgtga aatctacagt ataaagcaaa aattttatta aacctgtttt atatttatca
      481 tcaatacaac agatttaatt ccggaaagtc actcatcatc ccttgaatga acgtcatttg
      541 atgtattatt cttacacttg gattaattta agatgatacc tatgaaaaac tatcttttcc
      601 agacacccga agatatttgt gtacagttaa aaaaaatgac acatcctgtc acaataagaa
      661 caacagatat tgctaatttc tggcactatc ttgagtcagc aacacttcca gtgatcacaa
      721 aaagcaccac tacagaaaat cgggaggtta catttctgtg gcgctcagag aaagcactgc
      781 aaggcgtata tcttcgcctg aatcgtgtta cagataaaaa agacgtcaaa aaaggactaa
      841 tgactcatat cccttcgaca gatatctgga tgctgacact ggtgttacca gcttcatatc
      901 ggggctcata ctcatttata gaaattccca cagatatgac acaaaaagac atatttcaac
      961 taggaagtcg cttctctcca ttacccggta aatctgatcc atttaacaaa acagcagaaa
     1021 taaatatacg aggattcgga gaatcagtcc tttctcttga tatggctcct gaacaaaagg
     1081 aatgggatga tacttcccat aaatgtacag gtattctttc aacattacat tcctttgttg
     1141 caggatatca acgccggatt cgtttatatt ttcccctgaa tccaacatca gtacctcttg
     1201 gattacttgt gttacctgat gctgaaacat ggtttgaccg gatagatatt acccgggcat
     1261 tagatatggc catcaccact ggtcgtatca cgccaatggc aattatgggg atagacaata
     1321 ttaatgaatc tgatcgtatg aatatactgg gaggcaataa agaactcatc tttgatatag
     1381 cggaaaatct gataccccag ttatacagag actatccgga tatcgtatgg gctggtcgct
     1441 ctaatactgt actggccggg cagagcctcg gtggagtgac agcactgatg gcagctatat
     1501 atgcgtcgac aacatttggt acaatcatta gtcactcacc ttcaatgtgg tggaaccctg
     1561 accagggcag cccgatcttg tttactgaga ataatacctc ctgggtaaat gagcagatac
     1621 tttcagcgcc tccgaaagat gtaaatattc gacttggagt cggttcttta gaaggtacaa
     1681 ccgtctcaca tgttcagcga ttgcatcatt cgttaatcgc agctggtttg gaaagtaacc
     1741 tcactgtcta taccggtggt cacgattatg cctggtggcg cggagcaatt attgatgaat
     1801 tagcaaatta taattgcagg aaggtatcaa ataataactc tgtgtaaaat atcgttttat
     1861 cgtccgataa ctctttctgc aatattatta tgggagacta tctcatttat ttagtgatgt
     1921 ccttcattta tgtgatgaag gacattatat tactcataac atttctcgtt gaaaatttca
     1981 gcaatacgct acaattgttt cccgttgttt tttaattttc tcatcaaaac ctctgcaatc
     2041 tctgcaggac tgggttgttc agccatttca gcggctacct gatctgaaca gaagcgtagt
     2101 gaatcatcat aaagcaggcg atttaccaaa tcactggtca gtccatgctt gtccggaata
     2161 attccacacc cacgcttcgc aacaatttct gcattaacag agcgatctgc accttcgcca
     2221 aacacaatct gtggtattcc actatacagc gctgtcaggg tattacctgc gccaccgtga
     2281 tgaataaatc catcagctcc attaaggaat acacccatag gtatccagtc aaccagacgt
     2341 acatttgatg gtagttttcg taaaccagta cgagcattta ttgcaagttg caaaatgata
     2401 tcagcatcaa cttcatttgc agaatccata acccatgaaa tcagctccag accatcaacc
     2461 attggtttta cagtgccaag gctgataagt aaacgtttcc gatcagaatc cctgtcccac
     2521 catgtttcct ttacagcacc tccgttataa ggaatatatc tcattgagat aaccggttct
     2581 ccagcatttt taagaatgct catacttggg ggagcaacat caatccatgc taaatcacat
     2641 agtggaccgc tgactccatg ccgcctgtaa gcattgctta aagaacggtt taacatctga
     2701 atatgggcag atgtatgcgc gaatccaaca gccagcatca ctgaaggaat tctatattta
     2761 gcagcaacca atgggcctgc cggaccaaga gggggataga ctattaaatc tggcctccat
     2821 tttcctgcaa aatcgatgag gttatctgcc atttcatcgc taaaaaaaga gaaatgacca
     2881 ataatattac tttttttcct caactcttcc tgatggcgat atccagcctc tgaatctaaa
     2941 cctggaactg catcaaaaac aaccagtcca gcttctgctg ctttattcgc gaatttgcca
     3001 gcgctactaa ttactacatc atgtccaatt acacgaaagg cctgagccag agaaatcaat
     3061 gggtataaca aaccgtacag cggtgggcca ataaaaagaa tacgttttcg cattaaaaaa
     3121 gtccgtcttc tgcttacctg tttatttttc tatctctaag aaaacataat taatattatt
     3181 tttatgtgag cttatgcaca aaaataacca agtatattat cttctggtag agattcagga
     3241 tacagtttgt aggagctttc ttttttgaac aatgatttgc aggacagtta tgctaaacta
     3301 gcggaatcag tatcacaaca ataaatgact gatcatacct gaaatttgaa ttaacaggca
     3361 tcatgaatgt caatagtcta agcggtgact acttatatcg cttgcataaa tactaaacac
     3421 tgactagaaa agtattggat aaccccgtca ctaaaagtaa cgcttcggtt atttgctcgg
     3481 aatgtattaa cgccaagatt gttagccggt acgccttata tcactggcgc tgacaaatgt
     3541 ttcttttcta atttactgaa ccggcgcaga cacgcaggaa agcacgccgg caccggtgta
     3601 atgccagttc taatcaataa gaatggaatg acgaatcagt caggaaggga taacaacccg
     3661 ctgctgaacg gaaggtatca gcaggaagat gaacgggaat atcgccagca ccaagggcag
     3721 ggccgggttc cacaatacca gcgctggcat tttgcacatt gccagcgtac cggcattaca
     3781 acacgtttca tgctgctgcc ccccaggatt cctcgattcg ttgctacatc cactgctcta
     3841 cttcacttcc actgctccac ttcacttttg taccagcggc tgctgcgccc cagtttgatg
     3901 ggtttaggga aatggccttc actgatcaac ttgtaaaacc atttgtcggt cataccggtg
     3961 aaactggtga tatagttcat gtcgatcagg gaattatcgg cacgcagaaa tacgctgtcg
     4021 tggcttgttg tagtcaacat ggtcagaatg tcctgtgaga tttatgaaga ttgtgcgaat
     4081 gcggggaatc tactgagctg tgctttcaga actggcctgt tacgggatac cagggattac
     4141 cggcggggta acgggcttcc ggatcataca caccacgatt atcgcggaca aaatcactga
     4201 acgcccatat cacctcttta agtatgtctt cgcagtccgg taaatgacga tccagcgcca
     4261 catcccgggt ggtacagctt tgatgcgccc ggtgacacaa agcccggatt gttccagaca
     4321 tcctgaatca aacgccccag attaggggcg tcgaaatatg cctctctgac cattatattc
     4381 cggcgtatag gtagcaggtc tgaagtgaca atgcgtcacc tgacgttaaa agtcactaca
     4441 cccaagatga ccccagtggg ggcaacaagt ataaaaccgt tttttgatta agtggtcgga
     4501 tatgaaaagg ggaaggttta tgtgaaaaat acctgtgttt tctttgaaaa cacacaacag
     4561 tatcaggtta tttatgttta tacagtgcgt tccaaactga cctgaataat tacgcaagtt
     4621 gcaacaataa aacatttttg ccgtagtgtg aggtactccg gtaaattgct ttccaggtgc
     4681 agacaatgac taacgaagaa ctacaggcaa tcgcattatc catgcttgag cgccagcgcg
     4741 aaaaagtaca ccagcaacga gagtctttgc ttaacccatc tccattcacc aggccaaaac
     4801 gattggagct tgaagcattc ttggacaaat acccacggcg gcttaaatca ggcaaaacca
     4861 gaccaccagg ctgatagctc acagtttgtt ctccgattca ataaaacgga taagaaccat
     4921 cagcgcggta gcgtaaggca ctacagcgca ttgctttacg agtaataaca atggatttat
     4981 caccagaaga cgcaaagttt ttacgagaac tgcaacaccg tatgctcaga gaagccatta
     5041 tcgaacagca aacgggcatg agcgccatag acccattgcg atacaaacgg gcctcatctc
     5101 ctttagttga cgacaattcc aatgcggata aaacacgtcc accacactga ttacattttt
     5161 cattagcaca gtggtgtgat gcagcatacg aggaaaaccg gatgaatgag aacaaaataa
     5221 aaaggctgga gcaactgtta caggcacgcc aacgggagtt tgccactaaa tggggacgtg
     5281 ccgcgctgcc acatgaacgc ctaacctatt ttccacttga agacctcatt aaaaataatg
     5341 gcaaaaaaat caggtctgat aagaaacact tcttagaatg caatgaagac aaataacaac
     5401 aatctgcata ttgccaccaa ccacagatgt ggcatcgtta tcagcgcact tgattttctt
     5461 tatatccact gacgagcttg ccggggcacc tttgtttgcc ggtcattctg gtatcatgca
     5521 gatatttacg gcgtacaagc ccactagcat gacattgtaa taaggcctga gcctaacgct
     5581 ttaatgtgtg tcatacactc agtggcgtaa tcgttgacaa tatggtgcat gtaatcgtaa
     5641 gcgtccagcg aaatccatct ggtcataatc tgaagcatcc gacaaagtgg tgtccaccaa
     5701 ataagtgatc tgcgcccaat atcctgagcc ggaactttga caaaggccaa cgaaaaatgg
     5761 gagcggatgt tacagagttc tcggtacagg ggaaaagtgg tacctgtcgc cagttctccg
     5821 atctgtttaa ccgggaaatt atctcctaca gcttgtcaga aaggtcggtg atggagcatc
     5881 gttaatacga tgctatacga tgcattcaca gtgcccggcc agaggatgcc ccgtcgctgc
     5941 atatggatca gggttggcaa tatcgaatcg caggctatag gcaaagttaa agccccatgg
     6001 agtagcacaa aatatgccgc acaaaggaaa cggtctgaat aacgcagtga tgaagaactt
     6061 cttcagcaca cggctaaaac gcatagtgat cgagcgctga ttctagcgaa caactgaact
     6121 aatacatcag aatctgcatt atgttaaata aatataaaaa gatggtttaa ataccccgtt
     6181 acttttgact tacactatac ggtatcgcat cgtttaatat tcgcaccggc cagattttta
     6241 tttctattag ttgtcacaat actgaatgcg tacgaccaca gtattctggc tcctgtgtgg
     6301 ttatgcttta attctgcgtt ccgggcagat aagcagttgc ttgcaggaat ccttcttgtg
     6361 ttaatgtcag ttcccctttt taccagtgct gatttccaca ttccgtccaa cagagcttat
     6421 agcctttccc tggattatag cattgtccgg ctgaagttct ttttgaataa taatagaagc
     6481 actgctggca gatccagtcc gtttttcata acccactgta ctgataacca taatctaatc
     6541 agtagaaatt gagtcgaaaa taagcactac tccatacagg ataattagag gtcagtttga
     6601 ttattcacaa ttcatcatca gcattttcta tttctgacga aatcaatatg aaaataacca
     6661 tatatgataa ttattata
//
LOCUS       DAZFZC010000176         6637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFZC010000176 DAZFZC010000000
VERSION     DAZFZC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.178"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..175)
                     /locus_tag="ACHKXP_004793"
                     /pseudo
     CDS             complement(1..175)
                     /locus_tag="ACHKXP_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..1378
                     /locus_tag="ACHKXP_004794"
     CDS             506..1378
                     /locus_tag="ACHKXP_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7649620.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPVQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1750..4596
                     /locus_tag="ACHKXP_004795"
     CDS             1750..4596
                     /locus_tag="ACHKXP_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO7649621.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTVVQAGETVNGGTQTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGYALDTTLNGGNQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTIVNQKGKLQVNAGGIATNVTLKQGGALVTSTAATVTGSNRLGNFAVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQASNTTVGHRGTLMLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HRVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGNSAEGYNGQATLNVTF"
     gene            4717..>6637
                     /locus_tag="ACHKXP_004796"
     CDS             4717..>6637
                     /locus_tag="ACHKXP_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649622.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISLMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWQTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRVCA
                     SCGLTEVHHAPENHNPDPEWYCSSLCRETEILCQEIYERPYNCFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISA"
BASE COUNT         1582 a         1711 c         1841 g         1503 t
ORIGIN      
        1 gagttttcat ttatttttaa tgcataatca gatgccgcct catcattatc ctcagtatta
       61 tcatagccga aaacttcccc cggcacgggc gttcacgata atagtcatcg tcatagtttt
      121 catcgtcaga taatgatatg tcgtattctg aatatgattc actacagtta tacataaaat
      181 catctcctct gggatggtta caataattag tgagtttatt cgaaaaaata atgagcgcca
      241 gaccaatcca cccgactttt gattataatc atgaagatac ctgtctcagt acaaatagaa
      301 tcaaactcag tgcagcatga ttcaccttta tattctctca gggaaaccaa ataccactac
      361 tcaactatta agaacaaaat tctggaggta tgattatctg aagatacctg caccctaaat
      421 aatttcagac agatattatc gttgtgagtc ccccgccacc tttccggtgt gcggggtttt
      481 gtcttttttc accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa
      541 accgctctcc tccctgcctt actcgctttc ccgtcacatc ctggaacatc tgcgcaaact
      601 cacccgtcac gaacccgtga ttggcattat ggggaaaagc ggggccggta aatcctcact
      661 ctgtaatgca ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg
      721 ggaagtgcag cgcttccgtc tgagtggcca cggtcacagc atggttatca ctgacctgcc
      781 cggagtgggc gagagtcggg acagggatgc agagtatgaa gccctgtacc gtgacattct
      841 gcctgaactg gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga
      901 tgagtatttc tggcgacaca tcctgcaccg gggacatcag caggtgctgt ttgtggtgac
      961 gcaggccgac aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc
     1021 tgtacaggca cagaatattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt
     1081 acatccggtt gtggccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc
     1141 gctaatgaca gcacttcccg accatgccgc cagtcccctg atgacccgcc tgcaggacga
     1201 gctgcgcacg gaatctgtcc gcgctcaggc ccgtgaacag tttaccggtg cggtggaccg
     1261 gatatttgac acggcggaga gcgtctgtgt tgcctctgtt gcccgtacgg ttctgcgtgc
     1321 cgtccgtgac acggtggtct ctgttgcccg cgctgtatgg aactggattt tcttctgaac
     1381 agtctgccag ggaaatgcct gataacatta cgttaaccaa ctgaaaacat gacacaaacc
     1441 cctcatactc ctcctgtttc tcttgcgtga ccgccttgtc gttaatagaa taaaacgatc
     1501 gataaaacag gtatcgatcg ttactatcga tcgtttatag tggttaatgg tctgcgaaaa
     1561 ggcagctgaa tctcttcatc atgcagaacg gaattgcaca caacggactg atacttctct
     1621 ggcggatgag agaagggaag catttatgtc cgggaaaaac cactttatct gaccatgctg
     1681 ttttgtacct gccggtatcc acttttgtgg gtaccggctt ttttattcac cctctgtaag
     1741 gaaaagctga tgaaacgaca tctgaacacc agctacaggc tggtatggaa tcacattacg
     1801 ggcaccctgg tggtggcttc cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg
     1861 gttgtgctgt ctcttgctgc tgtcacatca gtcccggcac tggctgctga cacggttgta
     1921 caggcgggag aaaccgtgaa cggcggaaca cagacaaatc atgacaacca gattgttctc
     1981 ggtacggcca acggaatgac catcagtacc ggtctggagt atgggccgga taacgaggcc
     2041 aataccggcg ggcaatggat acaaaatggc ggtatcgcca acaacactac tgtcaccggt
     2101 ggtggtcttc agagagtgaa tgccggagga agcgtttcag acacggttat cagtgccgga
     2161 ggcggacaga gccttcaggg gcaggcagtg aataccactc tgaacggcgg tgagcagtgg
     2221 gtacatgaag gcgggattgc aacgggtacc gtcattaatg agaagggctg gcaggccgtc
     2281 aaatccggcg caatggcaac cgacacggtt gtgaataccg gcgcggaagg gggaccggat
     2341 gcagaaaatg gtgataccgg gcagtttgtt cgcggaaatg ccgtacgtac cactatcaat
     2401 gaaaatggtc gtcagattgt ggctgctgaa ggaacagcaa ataccactgt ggtttatgcc
     2461 ggcggcgacc agactgtaca tggctacgcg ctggatacca cactgaacgg cggtaaccag
     2521 tatgtgcaca acggcggtac agcctctgac actgttgtga acagtgacgg ctggcagatt
     2581 gtcaaggaag gtggtctggc ggatttcacc atcgttaacc agaaaggcaa actgcaggtg
     2641 aacgccggtg gtatagccac gaatgtcacc ctgaagcagg gaggcgcact ggtcaccagt
     2701 acggcggcaa ccgtcaccgg cagcaaccgt ctgggcaatt tcgcggtgga aaacggtaag
     2761 gctgacggcg ttgttctgga gtccggcggt cgcctggatg tactggaagg ccattcagcg
     2821 cagaaaacac gggtggatga cggcggaacc ctggcagtgt ctgccggtgg taaggcaaca
     2881 ggtgtcacca tgacatccgg tggtgccctg attgcagaca gtggtgccac tgttgagggg
     2941 accaatgcca gcggtaagtt cagtattgat ggcatatccg gtcaggccag cggcctgctg
     3001 ctggaaaatg gcggcagctt tacggttaat gccgggggac aggccagcaa caccactgtc
     3061 ggacatcgtg gaacactgat gctggctgcc gggggaagtc tgagtggcag aacacagctc
     3121 agtaaaggcg ccagcatggt actgaatggt gatgtggtca gtaccggcga tattgttaac
     3181 gcaggggaga tttactttga caatcagaca acaccggatg ccgtactgag ccgtgctgtt
     3241 gcaaaaggca acgccccggt aacgttccat aaactgacaa ccagtaacct caccggtcag
     3301 ggtggcacca tcaatatgcg tgttcgcctt gatggcagca atgcctctga ccagctggtg
     3361 attaatggtg gtcaggcaac cggcaaaacc tggcttgcgt ttacaaatgt cggaaacagc
     3421 aacctcgggg tggcaaccac cggacagggt atccgggttg tggatgcaca gaatggcgcc
     3481 accacagaag aaggtgcgtt tgccctgagt cgcccgcttc aggccggcgc ctttaactac
     3541 accctgaacc gtgacagcga tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct
     3601 gaagtccccc tgtatacatc catgttgaca caggcaatgg actatgaccg gattctggca
     3661 ggctcccgca gccatcagac cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt
     3721 cagggcggtc atctcggtca cgataacaac ggcggtattg cccgtggagc cacgccggaa
     3781 agcagcggca gctatggctt cgtccgtctg gagggtgacc tgctcagaac agaggttgcc
     3841 ggtatgtctc tgacgacagg ggtgtatggt gctgcaggcc attcttccgt tgatgttaag
     3901 gatgatgacg gttcccgcgc cggcacggtc cgggatgatg ccggcagcct gggcggatac
     3961 ctgaatctga cacacacgtc ctccggcctg tgggctgaca ttgtggccca gggaacccgc
     4021 cacagcatga aagcgtcatc ggacaataac gacttccgcg cccggggctg gggctggctg
     4081 ggctcactgg aaaccggtct gcccttcagt atcactgaca atctgatgct ggagccacaa
     4141 ctgcagtaca cctggcaggg actctccctg gatgacggcc aggataacgc cggttatgtg
     4201 aagttcgggc atggcagtgc acaacatgtg cgtgccggtt tccgtctggg cagccacaac
     4261 gatatgaact ttggtaaagg cacctcatcc cgtgacaccc tgcgcgacag tgcaaaacac
     4321 cgtgtgcgtg aactgccggt gaactggtgg gtacagcctt ctgttatccg caccttcagt
     4381 tcccggggtg acatgagcat ggggacagcc gccgccggca gtaacatgac gttctcaccg
     4441 tcccggaatg gcacgtcact ggacctgcag gccggactgg aagcccgtgt ccgggaaaat
     4501 atcaccctgg gcgttcaggc cggttatgcc cacagtgtca gcggcaacag cgctgaaggc
     4561 tataacggtc aggccacgct gaatgtgacc ttctgacaga accatcgcct ctttgtggtc
     4621 ccgatcatca tgatcgggac ccggaccggc gcaacggata ttcaacgcca cattcgctgg
     4681 cattaacaat aacatgatat tcatcacgga gtgactatgt tacagatagt cggcgcgctg
     4741 atcctgctga tagcaggatt tgccattctt cgccttttgt tcagagcatt aatcagcacg
     4801 gcttctgcgc tggcagggct tatattgctg tgtctgttcg gcccggcctt actggctggc
     4861 tatatcaccg aacgcataac ccggttgttc catattcgct ggctggcagg cgtatttctg
     4921 acgattgccg gaatgatcat cagcctcatg tggggacttg atggtaaaca tatcgcgctg
     4981 gaggctcaca cctttgactc tgtgaaattt attctgacca ccgctctcgc cggtggtctg
     5041 ctggctgttc ccctgcagat caaaaacatt cagcagaacg ggatcacacc agaagatatc
     5101 agcaaggaaa ttaacgggta ttactgctgt ttttatactg cctttttcct tatggcgtgt
     5161 tctgcatgcg caccattgat cgcattgcag tacgatattt caccgtcact gatgtggtgg
     5221 ggcgggttgt tgtactggct ggctgcatta gtgacgctgc tatgggcggc cagccaaatc
     5281 caggcgctga aaaaactgac ctgtgccatc agccagacac tggaagaaca accggtgctc
     5341 aacagtaaat cgtggcagac cagtttgcaa aacgattaca gccttcctga ctcactgacg
     5401 gagcgcatct ggctgacgct catttctcaa cggatttccc ggggagagct gagggaattt
     5461 gaactggcag acggaaactg gttactgaac aatgcctggt atgaaagaaa catggcaggg
     5521 tttaacgaac agttgaaaga gaacctgtca ttcacacctg atgaactgaa aacgctcttc
     5581 cggaaccgcc tgaatttatc accggaagcg aatgacgatt ttctcgatcg ttgcctggac
     5641 ggcggtgact ggtatccgtt ttcagaaggt cgccgttttg tatcattcca ccacgtggat
     5701 gagcttcgtg tctgtgcctc ctgcgggctg acagaagtac atcatgcccc ggaaaatcat
     5761 aatccggatc cggaatggta ctgctcctct ctttgtcgcg aaacagaaat actgtgtcag
     5821 gaaatttatg aacgccctta caactgtttt atttccgatg caacggcgaa tggtctgatt
     5881 ctcatgaagc tgccggaaac ctggagtaca aatgagaaaa tgtttgcttc cggagggcag
     5941 ggacatgggt ttgccgctga acggggaaac catattgtcg acagagtccg tctgaaaaac
     6001 gcacggatcc tcggtgataa taatgccaga aatggagcag acagactggt cagcggaaca
     6061 gaaatccaga cgaaatattg ttcaactgca gcccgtagcg tcggtgcggc attcgacggg
     6121 caaaacggac agtatcgtta catgggaaat aatggcccca tgcaactgga agtcccccgt
     6181 gatcagtatg ccggcgctgt tgaaactatg aggaataaga tccgcgaagg taaagtaccc
     6241 ggtgtaaccg atcccgcaga agcgtcccgg ctgattcgtc ggggacatct gacttatacc
     6301 caggcccgta atatcacccg gttcgggacc atcgaatcgg tcacttatga tattgccgag
     6361 ggttcggttg tcagtctggc ggccggaggg atcagttttg ccctgacggc atcggtcttc
     6421 tggctcagca ctggcgatcg cgatgctgcc ctgcagacag ctgctgtcca ggcaggaaaa
     6481 accttcaccc gcacactggc tgtctacgtc acaacccagc aacttcaccg gctcagtgtt
     6541 gttcagggta tgctgaagca cattgatttt tcgactgcca gcccgactgt ccgactggct
     6601 cttcagaagg ggactggtgc aggaaatatc agtgccc
//
LOCUS       DAZFZC010000177         6628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFZC010000177 DAZFZC010000000
VERSION     DAZFZC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.179"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..962)
                     /locus_tag="ACHKXP_004797"
     CDS             complement(<1..962)
                     /locus_tag="ACHKXP_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7649623.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLIFRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWVCGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNG"
     gene            939..1180
                     /locus_tag="ACHKXP_004798"
                     /pseudo
     CDS             939..1180
                     /locus_tag="ACHKXP_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1261..1419)
                     /locus_tag="ACHKXP_004799"
     CDS             complement(1261..1419)
                     /locus_tag="ACHKXP_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7649624.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1505..2248)
                     /locus_tag="ACHKXP_004800"
     CDS             complement(1505..2248)
                     /locus_tag="ACHKXP_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649625.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(2433..3122)
                     /locus_tag="ACHKXP_004801"
     CDS             complement(2433..3122)
                     /locus_tag="ACHKXP_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO7649626.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(3137..3259)
                     /locus_tag="ACHKXP_004802"
     CDS             complement(3137..3259)
                     /locus_tag="ACHKXP_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO7649627.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            3597..4556
                     /locus_tag="ACHKXP_004803"
     CDS             3597..4556
                     /locus_tag="ACHKXP_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO7649628.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(4700..4771)
                     /locus_tag="ACHKXP_004804"
                     /pseudo
     CDS             complement(4700..4771)
                     /locus_tag="ACHKXP_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(4768..5433)
                     /locus_tag="ACHKXP_004805"
     CDS             complement(4768..5433)
                     /locus_tag="ACHKXP_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO7649629.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(5430..6041)
                     /locus_tag="ACHKXP_004806"
     CDS             complement(5430..6041)
                     /locus_tag="ACHKXP_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7649630.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(6034..6204)
                     /locus_tag="ACHKXP_004807"
     CDS             complement(6034..6204)
                     /locus_tag="ACHKXP_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO7649631.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            complement(6201..6383)
                     /locus_tag="ACHKXP_004808"
     CDS             complement(6201..6383)
                     /locus_tag="ACHKXP_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7649632.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            complement(6380..>6628)
                     /locus_tag="ACHKXP_004809"
     CDS             complement(6380..>6628)
                     /locus_tag="ACHKXP_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFO7649633.1"
                     /translation="MLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTIVSKAALMAIGQGVRRAA"
BASE COUNT         1897 a         1617 c         1312 g         1802 t
ORIGIN      
        1 ccgttaccat ctgtcatgaa gggcacaaaa taaacgccct cactctccct gtttttatac
       61 ccgccgtaca cggtgtcgta ctgggtagcg tatgtatttt tccagtaata cgtcgtgtca
      121 ccacaaaccc acggcacatc tgcagcactg ccaccatggc actgcgcgtt aaacacggag
      181 aggtcagcac gaaactgtgt cagcatggct gtaaacagcg caggttgctg tgcgtgggtg
      241 gcggcgctca tgtcaaactc accctgcatc cagcagacgg ccagcagaac gtttttggga
      301 tttttctgca atgccgcttt tgtgcggaaa atcagatcct gatataacgg cttgcccacc
      361 ccccagcgtg ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgcgccc
      421 tgggtaaatg ccgaaccacc acgacagcat ggtaccagca ggatccccgc gttattcggg
      481 atatacggga gcagtttttt ggcaatatgt aaaccctgac cgacacagcc gtactgccct
      541 ttgctcaggt cagccctcgg atgattcagc gtactcatat cctgcacatc atgcagacag
      601 tggtcagccg gaataatatc gttatatctg caggcagccc cgcccggcgt cactgtactg
      661 cggcgcgcca gctgtttaat gcgcggatcc ggagcatcgt atgaatccgg cagcggaagc
      721 ccttcaccgt aagccatggc attggactgc ccggccagta cgatgacgta gtaccaatcc
      781 ggctcagatg aagggccgac ctgtggctct ccttcaatag ccaccgcctg catcagtgtg
      841 tacggcgtaa tggcaaccgg tccgccgtat ggctgccagc cctctttcag tttgtgtgtc
      901 agcttttccg caaggtctga cggcgacgcc gccctgacaa catcgtaatg tttaatcgac
      961 atcgaatttc tcccgtgtag aggaacagag ttaaaaagcc ggaagcggaa tcaaatcaca
     1021 ggatgaccat ctgccagtgg caggtcataa aaaaaaggct gcgcaatgcg cagccagaac
     1081 tcacaaggaa aatgataaaa ggaataacac tagtgatgta cgcatggcgc ctcccgctaa
     1141 gttctgcaat gatcaaacag aactcgctac gtgcccttaa aactcgatca tttagcccct
     1201 ccaaggagga ttcaccatgc ggttgatttt ttaataaaca gtaaacaaaa aagtcaagaa
     1261 ttattcattc tgttctttca tcatcggcca cagcaatacc acaatgccgc agaccagagc
     1321 gccatcagtc agtaccaaca ttatcctgct ggtgaaatcc atcatcacca tcactaaaag
     1381 caggatcaca acagcaagca gacacagttt ataaaacaat gttcagaaaa cgcattcagc
     1441 atgcctaagg ttctattcct acgaatagcc aacttgcaac ttaaaatatt atttatgcag
     1501 ccaattaaat tctggtcctt acaatatcaa cctgaagatt cttatcttgt gctgattgat
     1561 aaatgacaaa ccttttacta cctgcattga aagaagtaga caaaaccaga caattatcat
     1621 aacgagcaag aacataatac caaccatcat tataattaat catttcatat tctttcttaa
     1681 actgtggttt gtaatatcct gtcagaaatg aaaaaagcca aaaatatgcc acaaaagcaa
     1741 tcatcacaat ctcaaaaaaa tgttttttta taaatggctt atcatagaag catgataccg
     1801 ataaaaatcg cccataagat cttatcgaaa ttgtaaccgc cagcgcaatc gctgctgaca
     1861 gtagcaaaag aggtacctga atcttctgtc tcaatataga aaactcaata attgccggca
     1921 caaacaataa ttccacagca aaataaaggc gaaatacatt tagctcttgc atagaatgtt
     1981 ttcttttcac tgcgaaaaag aatacaacac caatacccca accgataaga aatatagcaa
     2041 tgacgataac tgcaaaaaat aaacttctgg caacatcatc aacacctgca cctacaatcc
     2101 accatgggaa gccgtagtaa aaagaagtac cccatccata gaaataagca ctcccccatc
     2161 caaggcatcc catgtaggca ataaaaagtg aagaactcct gagcagcgca ccatccttca
     2221 taaccacccc aatacaagat gataacattg gcttacaact cataacaaaa gcaattcaat
     2281 gccgtcaaga ggttacaggc taaaaaaact ctattacata gcagccagca tgtttaccgt
     2341 acaagtacaa ctcagggcat aaaaaaaacc cactcggcag cgggtttata cattttttac
     2401 aacataccaa atttgcatga agtatatggc ttttaatcca gttttgcaat attttgctgt
     2461 aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa gcaataaagc atcactatca
     2521 agctgtagaa aaatgtgctt cattgcaacc cagcgttcag taaatgtctc ggaccagttt
     2581 tttgttgtca ctcccaccaa tgatgccagt gtctggtatt cataggcctc acgccctgca
     2641 agttcgctct tcacatcctg tgcagccagc cagattaacg tcttcaggcg atccagtgtc
     2701 ttacctgcaa tttttctgtt acttaacaaa tctttaaact cgctccatgc ccattgcgtt
     2761 atggtgacct gatgccccca tcgaacgctt tcgctgtaac accaaagcaa ccatgctttc
     2821 tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag aggttgagaa ttcaaccggg
     2881 ctgaccaaag caatggatga accttttgcg tacgactgct taccggaagt cggcgtatta
     2941 tccagcgtaa tcatcttgcc agttaccaca tccagaatgc gcggcttctt tcgtttgtat
     3001 gtaccagtat caaattgtgc atgctcctgc caggcttcga gctggccttt cgttgctccg
     3061 ttcaagtcag cagtagctgc cataagttgc tcacgaacat actgtaaata ttgggtattc
     3121 atgcagtaaa tcctttctat attttggcat aattcttcaa cattcggtaa tcgttcaaaa
     3181 ccgaatcggg gaaacgacat aagcacagga gcccccagcg acagcgaagg agttctgata
     3241 tataagactc agacatcatt cattccccgg ttctccaata tctgtttcac tcatcatcca
     3301 taactacctg taattgcccc cctttttttg taacagttct tatattgcta tatagaatag
     3361 ccattactaa tgcatttaaa tttaataaaa taaaaattat aaaaaacata aaacaccacg
     3421 caaacacact taataaaaac accgttacat taaaagataa taaaaaccgc aataaaaaac
     3481 gaataaatca attgtctcac gcaattataa aacatcatat tgattacgca ccttgtatta
     3541 caaactcatg tatgtaaaat acgcgcacca ttcaaaaaaa aggaagacaa taacatatga
     3601 aaaaaagtgt catcgctggc gtctttattg ctctgtcatt taccacgtgt tcagctatcg
     3661 cgaacagcct tgcattatca ttagcaaatg atgatgcagg gaagtttcaa ccaatactta
     3721 atgatattta tggcaataaa catgaaaaca gagatgatta ctcacaaggc ttatttctgg
     3781 gatatagcca cgatatctca gactcgagcc aattatctct ccatattgcg caagatattt
     3841 actctccatc aggcagtaat aaaagacaca acacagctgt aactggagac agagctttta
     3901 gtgcatacac tcacactggt attgaatgga actcccttgc gaatgactgg attcgctatc
     3961 gattaggtac tgacataggt gttgttggcc ccgacgcagg cggtcagaaa gtacaaaata
     4021 aagctcatga gattattggg gcagaaaaat atcatgcatg ggatgatcaa atagagaatc
     4081 gctacggtta tactgtaaaa gggatgctat ccatgacacc aagtatggat attttaggtg
     4141 ctaatgttgg attataccct gaagtttctg ctgttactgg aaacttattt caatatgtag
     4201 catatggcgc aaccattgcc attggtaatg ataaaacctt caattcggat aatggctttg
     4261 gtctgctggc tccccgtggt ttaatgcata tgtccgatac aagcggattc aaatacaaga
     4321 tttttgcagg tatggaaaga cgagatgtca atcgcaacta tactcttgaa ggaaaaacaa
     4381 tacagacgaa acaaacaaca gtatcgctaa acaaaactgt tgatgaatat caagttggcg
     4441 caacaattgg gtatgcacct gtagccttca cactagcatt taataaagta acatcagaat
     4501 tcaagacagg ggatgactat tcatttataa atggagcaat caccttcttt ttttaactga
     4561 attgaattca atcaaaataa cataagtcca acaaaaacat aaagtgcgaa atgaatgcca
     4621 gctccattta tttcgcacta taaaagatta aaagttgcaa taaaataata aaatgactca
     4681 gttacgaaaa ccaataaact gtggccagta gtgagtcgct catcatcggg ctttttggcg
     4741 aatgaaattt agctacgctt tcgagtctca tgcgccttct ccctgtacct gaatcaatgt
     4801 gaggtttccg cagaacactg cgccagtatc gatatacatc tggttggcaa atttgagtgg
     4861 tttcactgct ggcgtatgac caaagatgaa cgtgtccgcg cctttgattt ctttcacgat
     4921 cccgtcttgt gagttgctga ttcgttcgcg gttccagatt acctgctgat gatcaactgg
     4981 ctttccaaac tcgtatttat cacaaggata atcggcgtgg caaatgacat attttttatc
     5041 tttgctcacc agttcgatga ttaacggaag ttcttctgct ttatgggcaa gaactttagc
     5101 cagagtctct ttgtcgtaat cgagattaaa gaaccagcca ccgccattaa gcagccagtg
     5161 attaacgttt ccacgctctg ataagccatc aatcatcatt tgctcatggt ttccacgcac
     5221 agctctgaac caggggaatg tgattaattc cagacattcg acgttctctg taccgcgatc
     5281 aaccaaatcg ccaaccgaga tgagcaggtc ttttttggtg tcgaatccaa tcgtatccag
     5341 tttgttcatc aggttcgtgt agcatccgtg caggtcgcca gctacccaaa tatttcggta
     5401 tttgctgcca tcaattcttt cgtagatatt catgcagcct cacttctgct gtttcgcagg
     5461 tctttgagtt tctgttggta ctctgccttg atcgccttgc actcttcgac agtccagcga
     5521 tggcggttat ggtttgattc gatttcgtct actgcttcct gcccgatgcg gctaatcagt
     5581 tcgacgcgat acggaacgag atttccgctt ttgtgctggt tgcacaccac gcattgcttg
     5641 tgaatattgc gttcatcaaa tcggagttga ggtgccgcag cagttgtccg gtaatgtccg
     5701 gcatcccact gagcagacgt gagcgttccg cacgagatac atggtaagtc gcggtctctt
     5761 tctctgatga aggcgtttac ggcttgttgg gcttgtttaa tccagtaact gcggggcttt
     5821 aaggcgagtt ttcgaatctt aagtttatct ttctgtttct gctcctctcg tcgtcgtttc
     5881 ttctctgctg ctttttccgc tttttcgcgt tctttacttc gtcgttcgag tgctatcttg
     5941 gttccacact ctggagagca ccaccactga ttagcgaatg cagggtgaaa ccattcccga
     6001 cattcatcgt ttttacatcg tcttcgcgct ggtttagcca tcatcttctt cctcgtgcat
     6061 cgagctattc gaatcgctca tcagctctgc acagcagtgc tcacacacgt gaacttccag
     6121 cacatgcagc ttctgaccgc aattagcgca cgttaaagcc cgctcgacgc tttcttgttc
     6181 gtaacttcga tttgggtcaa tcaccttgta ttcctcgcac gatgtcttag ccaccggata
     6241 tcccacaggt gagccgtgta gttgaaggtt tttacgtcag attcttttgg gattggcttg
     6301 cgtttatttc tggagcgttt cgttggaagg tatttgcagt tttcgcagat gatgtcggtg
     6361 aaacttcgtc gttgtcgcct catgccgccc tcctgacgcc ctgcccgatc gccatcaatg
     6421 ccgctttgga tacgatagta aacatccgtc gaggactaat gaacggtcgc caaatcagca
     6481 gcatggagcc tttgctgttt cccttcttct ccagccctgt cgatggttcg ataaaattaa
     6541 tccgtccatc agtgataata cgaacttcgt caacactctc cagagccttg ctgaaccatc
     6601 cgacagacat atcctctggc acaagcat
//
LOCUS       DAZFZC010000178         6617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFZC010000178 DAZFZC010000000
VERSION     DAZFZC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.180"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..672
                     /locus_tag="ACHKXP_004810"
     CDS             112..672
                     /locus_tag="ACHKXP_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7649634.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            675..3641
                     /locus_tag="ACHKXP_004811"
     CDS             675..3641
                     /locus_tag="ACHKXP_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFO7649635.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            3643..4365
                     /locus_tag="ACHKXP_004812"
     CDS             3643..4365
                     /locus_tag="ACHKXP_004812"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HFO7649636.1"
                     /translation="MLYFPFSETTPSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVER
                     IDADQWRIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGET
                     ADLAPTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSD
                     MALSKGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGRE
                     VMGIVQGGSVSWQLGRQRGLER"
     gene            4582..5796
                     /gene="floR"
                     /locus_tag="ACHKXP_004813"
     CDS             4582..5796
                     /gene="floR"
                     /locus_tag="ACHKXP_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HFO7649637.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSILAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            5824..6129
                     /locus_tag="ACHKXP_004814"
     CDS             5824..6129
                     /locus_tag="ACHKXP_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7649638.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            6241..>6617
                     /locus_tag="ACHKXP_004815"
     CDS             6241..>6617
                     /locus_tag="ACHKXP_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO7649639.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVL"
BASE COUNT         1291 a         2030 c         1984 g         1312 t
ORIGIN      
        1 tgatgtcaga tgccatgtgt aaattgcgtc aggataggat tgaattttga atttattgac
       61 atatctcgtt gaaggtcata gagtcttccc tgacattttg cagggaattc catgactgga
      121 cagcgcattg ggtatatcag ggtcagcacc ttcgaccaga acccggaacg gcaactggaa
      181 ggcgtcaagg ttgatcgcgc ttttagcgac aaggcatccg gcaaggatgt caagcgtccg
      241 caactggaag cgctgataag cttcgcccgc accggcgaca ccgtggtggt gcatagcatg
      301 gatcgcctgg cgcgcaatct cgatgatttg cgccggatcg tgcaaacgct gacacaacgc
      361 ggcgtgcata tcgaattcgt caaggaacac ctcagtttta ctggcgaaga ctctccgatg
      421 gcgaacctga tgctctcggt gatgggcgcg ttcgccgagt tcgagcgcgc cctgatccgc
      481 gagcgtcagc gcgagggtat tgcgctcgcc aagcaacgcg gggcttaccg tggcaggaag
      541 aaatccctgt cgtctgagcg tattgccgaa ctgcgccaac gtgtcgaggc tggcgagcaa
      601 aagaccaagc ttgctcgtga attcggaatc agtcgcgaaa ccctgtatca atacttgaga
      661 acggatcagt aaatatgcca cgtcgttcca tcctgtccgc cgccgagcgg gaaagcctgc
      721 tggcgttgcc ggactccaag gacgacctga tccgacatta cacattcaac gataccgacc
      781 tctcgatcat ccgacagcgg cgcgggccag ccaatcggct gggcttcgcg gtgcagctct
      841 gttacctgcg ctttcccggc gtcatcctgg gcgtcgatga actaccgttc ccgcccttgt
      901 tgaagctggt cgccgaccag ctcaaggtcg gcgtcgaaag ctggaacgag tacggccagc
      961 gggagcagac ccggcgcgag cacctgagcg agctgcaaac cgtgttcggt ttccggccct
     1021 tcaccatgag ccattaccgg caggccgtcc agatgctgac cgagctggcg atgcaaaccg
     1081 acaaaggcat cgtgctggcc agcgccttga tcgggcacct gcggcggcag tcggtcattc
     1141 tgcccgccct caacgccgtc gagcgggcga gtgccgaggc gatcacccgt gctaaccggc
     1201 gcatctacga cgccttggcc gaaccactgg cggacgcgca tcgccgccgc ctcgacgatc
     1261 tgctcaagcg ccgggacaac ggcaagacga cctggttggc ttggttgcgc cagtctccgg
     1321 ccaagccaaa ttcgcggcat atgctggaac acatcgaacg cctcaaggca tggcaggcac
     1381 tcgatctgcc taccggcatc gagcggctgg ttcaccagaa ccgcctgctc aagattgccc
     1441 gcgagggcgg ccagatgaca cccgccgacc tggccaaatt cgagccgcaa cggcgctacg
     1501 ccactctcgt ggcgctggcc accgagggca tggccaccgt caccgacgaa atcatcgacc
     1561 tgcacgaccg catcctgggt aagctgttta acgctgccaa gaataagcat cagcagcagt
     1621 tccaggcgtc aggcaaggcc atcaacgcca aggtacgtct gtacgggcgc atcggtcagg
     1681 cgctgatcga cgccaagcaa tcaggccgcg atgcgtttgc cgccatcgag gccgtcatgt
     1741 cctgggattc ctttgccgag agcgtcaccg aggcgcagaa gctcgcgcaa cccgatgact
     1801 tcgatttcct gcatcgcatc ggcgagagct acgccaccct gcgccgctat gcaccggaat
     1861 tccttgccgt gctcaagctg cgggccgcgc ccgccgccaa aaacgtgctt gatgccattg
     1921 aggtgctgcg cggcatgaac accgacaacg cccgcaagct gccagccgat gcaccgaccg
     1981 gcttcatcaa gccgcgctgg cagaaactgg tgatgaccga cgccggcatc gaccggcgct
     2041 actacgaact gtgcgcgctg tccgagttga agaactccct gcgctcgggc gacatctggg
     2101 tgcagggttc acgccagttc aaggacttcg aggactacct ggtaccgccc gagaagttca
     2161 ccagcctcaa gcagtccagc gaattgccgc tggccgtggc caccgactgc gaacaatatc
     2221 tgcatgagcg gctgacgctg ctggaagcac aacttgccac cgtcaaccgc atggcggcag
     2281 ccaacgacct gccggatgcc atcatcaccg agtcgggctt gaagatcacg ccgctggatg
     2341 cggcggtgcc cgacaccgcg caggcgctga tagaccagac agccatggtc ctgccgcacg
     2401 tcaagatcac cgaactgctg ctcgaagtcg atgagtggac gggcttcacc cggcacttca
     2461 cgcacttgaa atcgggcgat ctggccaagg acaagaacct gttgttgacc acgatcctgg
     2521 ccgacgcgat caacctgggc ctgaccaaga tggccgagtc ctgccccggc acgacctacg
     2581 cgaagctcgc ttggctgcaa gcctggcata cccgcgacga aacgtactcg acagcgttgg
     2641 ctgaactggt caacgctcag tttcggcatc cctttgccgg gcactggggc gatggcacca
     2701 catcatcatc ggacggacag aatttccgaa ccgctagcaa ggcaaagagc acggggcaca
     2761 tcaacccaaa atatggcagc agcccaggac ggactttcta cacccacatc tccgaccaat
     2821 acgcgccatt ccacaccaag gtggtcaatg tcggcctgcg cgactcaacc tacgtgctcg
     2881 acggcctgct gtaccacgaa tccgacctgc ggatcgagga gcactacacc gacacggcgg
     2941 gcttcaccga tcacgtcttc gccctgatgc acctcttggg cttccgcttc gcgccgcgca
     3001 tccgcgacct gggcgacacc aagctctaca tcccgaaggg cgatgccgcc tatgacgcgc
     3061 tcaagccgat gatcggcggc acgctcaaca tcaagcacgt ccgcgcccat tgggacgaaa
     3121 tcctgcggct ggccacctcg atcaagcagg gcacggtgac ggcctcgctg atgctcagga
     3181 aactcggcag ctacccgcgc cagaacggct tggccgtcgc gctgcgcgag ttgggccgca
     3241 tcgagcgcac gctgttcatc ctcgactggc tgcaaagcgt cgagctacgc cgccgcgtgc
     3301 atgccgggct gaacaagggc gaggcgcgca atgcgctggc ccgtgccgtg ttcttcaacc
     3361 gccttggtga aatccgtgac cgcagtttcg agcagcagcg ctaccgggcc agcggcctca
     3421 acctggtgac ggcggccatc gtgctgtgga acacggtcta cctggagcgt gcggcgcatg
     3481 cgttgcgcgg caatggtcat gccgtcgatg actcgctatt gcagtacctg tcgccactcg
     3541 gctgggagca catcaacctg accggtgatt acctatggcg cagcagcgcc aagatcggcg
     3601 cggggaagtt caggccgcta cggcctctgc aaccggctta gcgtgcttta ttttccgttt
     3661 tctgagacga ccccttccta tacgtgccgc gttccaggcg gcgactatga gggctatgtc
     3721 gatgcccatg tgcgccggct ggaggcgcta cgccgggccg gtatcgtcga gcggatcgac
     3781 gccgaccaat ggcgcatccc cgatgatctg gtcagccgtg ccgccgccca tgacgccggc
     3841 cgagacagtc aggccagcgt tcgcgtcctt tccccggtcg atctgaacaa acagatcgga
     3901 tcggacggcg cgacctggct ggaccggcgg ctgatccacg gcgagacggc cgaccttgcg
     3961 ccaaccggct tcgggcaaca agtccgcgaa gccatggacc agcgccgcga gcaccatatc
     4021 gaacagggcg acgccacccg cagccgggac agccgcgtct tctaccggcg caaccttctc
     4081 gccatcctgc gggagcgcga ggtagccggc gtcggatcgg atatggcttt gagtaagggc
     4141 ctgccgttcc gcgccgccac ggacggcgag agcgtcagcg gcaagtttac cggaaccgtg
     4201 catctatcga gcggcaagtt cgccgtggtc gagaaatccc atgagttcac ccttgtcccg
     4261 tggcggccga tcatcgaccg ccaactcggc cgcgaggtta tgggcatcgt gcagggcggg
     4321 tcggtgtcgt ggcagttagg gcggcagagg gggctggaac gctgagtgcg cccatgccgc
     4381 attgcgaagc aaaagataat cggataaaat gtagcaattc atattcgtaa gcgtggagta
     4441 atcagatggg aaattccaag tcagcagaca agtaagccgc aacaaccagt attgttgttg
     4501 cggcgctctg taaggctagt ctcatctgat tgctgacgag cagacgtcgc ccggtattcc
     4561 ttaatcgagg gttgattcgt catgaccacc acacgccccg cgtgggccta tacgctgccg
     4621 gcagcactgc tgctgatggc tcctttcgac atcctcgctt cactggcgat ggatatttat
     4681 ctccctgtcg ttccagcgat gcccggcatc ctgaacacga cgcccgctat gatccaactc
     4741 acgttgagcc tctatatggt gatgctcggc gtgggccagg tgatttttgg tccgctctca
     4801 gacagaatcg ggcgacggcc aattctactt gcgggcgcaa cggctttcgt cattgcgtct
     4861 ctgggagcag cttggtcttc aactgcaccg gcctttgtcg ctttccgtct acttcaagca
     4921 gtgggcgcgt cggccatgct ggtggcgacg ttcgcgacgg ttcgcgacgt ttatgccaac
     4981 cgtcctgagg gtgtcgtcat ctacggcctt ttcagttcga tactggcgtt cgtgcctgcg
     5041 ctcggcccta tcgccggagc attgatcggc gagttcttgg gatggcaggc gatattcatt
     5101 actttggcta tactggcgat gctcgcactc ctaaatgcgg gtttcaggtg gcacgaaacc
     5161 cgccctctgg atcaagtcaa gacgcgccga tctgtcttgc cgatcttcgc gagtccggct
     5221 ttttgggttt acactgtcgg ctttagcgcc ggtatgggca ccttcttcgt cttcttctcg
     5281 acggctcccc gtgtgctcat aggccaagcg gaatattccg agatcggatt cagctttgcc
     5341 ttcgccactg tcgcgcttgt aatgatcgtg acaacccgtt tcgcgaagtc ctttgtcgcc
     5401 agatggggca tcgcaggatg cgtggcgcgt gggatggcgt tgcttgtttg cggagcggtc
     5461 ctgttgggga tcggcgaact ttacggctcg ccgtcattcc tcaccttcat cctaccgatg
     5521 tgggttgtcg cggtcggtat tgtcttcacg gtgtccgtta ccgcgaacgg cgctttggca
     5581 gagttcgacg acatcgcggg atcagcggtc gcgttctact tctgcgttca aagcctgata
     5641 gtcagcattg tcgggacatt ggcggtggca cttttaaacg gtgacacagc gtggcccgtg
     5701 atctgttacg ccacggcgat ggcggtactg gtttcgttgg ggctggtgct ccttcggctc
     5761 cgtggggctg ccaccgagaa gtcgccagtc gtctaaccga cgactggtag caggcccgct
     5821 ccgatgcggc gcactaacca tcgaaacctc gtgaatgtcg gtatcctgtc tggcaggata
     5881 ccgctcattt cccttgttca gttcatcgcc gtcgccgagc atctgaattt tcggcatgca
     5941 gccaaggcac ttggtatcag ccagtcgagc gtcagcgcgc gtgtgaaagc gctggaggat
     6001 aaccttggtg tcctgctatt tgagcgccat gcgcggggcg ttcggctaac agacgcaggc
     6061 aggcacttca tggagcgtgt cacggcgggt gtcgatcaac tcgatcacgc agtgaagacc
     6121 gcggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg gctgagggta
     6181 aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag gacgcggaac
     6241 atgcctcatg tggcggccag gacggccagc cgggatcggg atactggtcg ttaccagagc
     6301 caccgacccg agcaaaccct tctctatcag atcgttgacg agtattaccc ggcattcgct
     6361 gcgcttatgg cagagcaggg aaaggaattg ccgggctatg tgcaacggga atttgaagaa
     6421 tttctccaat gcgggcggct ggagcatggc tttctacggg ttcgctgcga gtcttgccac
     6481 gccgagcacc tggtcgcttt cagctgtaag cgtcgcggtt tctgcccgag ctgtggggcg
     6541 cggcggatgg ccgaaagtgc cgccttgctg gttgatgaag tactgcctga acaacccatg
     6601 cgtcagtggg tgttgag
//
LOCUS       DAZFZC010000179         6282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFZC010000179 DAZFZC010000000
VERSION     DAZFZC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.181"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            86..1015
                     /gene="pfkB"
                     /locus_tag="ACHKXP_004816"
     CDS             86..1015
                     /gene="pfkB"
                     /locus_tag="ACHKXP_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO7649640.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1115..1405
                     /gene="ghoS"
                     /locus_tag="ACHKXP_004817"
     CDS             1115..1405
                     /gene="ghoS"
                     /locus_tag="ACHKXP_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7649641.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDKG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1511..2371
                     /gene="yniA"
                     /locus_tag="ACHKXP_004818"
     CDS             1511..2371
                     /gene="yniA"
                     /locus_tag="ACHKXP_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO7649642.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2412..2948)
                     /gene="yniB"
                     /locus_tag="ACHKXP_004819"
     CDS             complement(2412..2948)
                     /gene="yniB"
                     /locus_tag="ACHKXP_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO7649643.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3095..3763
                     /gene="hxpB"
                     /locus_tag="ACHKXP_004820"
     CDS             3095..3763
                     /gene="hxpB"
                     /locus_tag="ACHKXP_004820"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO7649644.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3926..4516
                     /gene="ydjM"
                     /locus_tag="ACHKXP_004821"
     CDS             3926..4516
                     /gene="ydjM"
                     /locus_tag="ACHKXP_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7649645.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4649..6040
                     /gene="tcyP"
                     /locus_tag="ACHKXP_004822"
     CDS             4649..6040
                     /gene="tcyP"
                     /locus_tag="ACHKXP_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO7649646.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6044..>6282)
                     /gene="ydjO"
                     /locus_tag="ACHKXP_004823"
     CDS             complement(6044..>6282)
                     /gene="ydjO"
                     /locus_tag="ACHKXP_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO7649647.1"
                     /translation="SGILEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLAIF
                     FADDLFQYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1542 a         1551 c         1578 g         1611 t
ORIGIN      
        1 ataaagctcc aataaatcat attgttaatt tcttcacttt ccgctgattc ggtgccagac
       61 tgaaattagc ctataggagg aaatgatggt acgtatctat acgttgacac ttgcgccctc
      121 tctcgatagc gcaacaatta ccccgcaaat ttatcccgaa ggaaaactgc gctgtaccgc
      181 accggtgttc gaacccgggg gcggcggcat caacgtcgcc cgcgccattg cccatcttgg
      241 aggcactgcc acagcgatct tcccggcggg tggcgcgacc ggcgaacatc tggtttcact
      301 gttggcggat gaaaatgtcc ccgtcgctac tgtagaagcc aaagactgga cccggcagaa
      361 tttacatgta catgtggaag caagcggtga gcagtatcgt tttgttatgc caggcgcggc
      421 attaaatgaa gatgagtttc gccagcttga agagcaagtt ctggaaattg aatccggggc
      481 catcctggtc ataagcggaa gcctgccgcc aggtgtgaag ctggaaaaat taacccaact
      541 gatttccgct gcgcaaaaac aagggatccg ctgcatcgtc gacagttctg gcgaagcgtt
      601 aagtgcagca ctggcaattg gtaacatcga gttggttaag cctaaccaaa aagaactcag
      661 tgcgctggtg aatcgcgaac tcacccagcc ggacgatgtc cgcaaagccg cgcaggaaat
      721 cgttaatagc ggcaaggcca aacgggttgt cgtttccctg ggtccacaag gagcgctggg
      781 tgttgatagt gaaaactgta ttcaggtggt gccaccaccg gtgaaaagcc agagtaccgt
      841 tggcgctggt gacagcatgg tcggcgcgat gacactgaaa ctggcagaaa atgcctctct
      901 tgaagagatg gttcgttttg gcgtagctgc ggggagtgca gccacactca atcagggaac
      961 acgtctgtgc tcccatgacg atacgcaaaa aatttacgct tacctttccc gctaacaaaa
     1021 acattccccc agcattgggg gaatcaccac caactgtcgg caacgcgttt ctccgactat
     1081 gctcaaaagt catgtgataa caaaggggtg aactatggcc agtggcgatc ttgtccgtta
     1141 tgtcataacc gtcatgttgc atgaggatac attgactgaa attaacgagt tgaataatta
     1201 cctgactcgc gacggttttt tgctcaccat gacggatgat aagggaaata tccatgaact
     1261 ggggactaac acttttggac ttatcagtac ccaaagtgaa gaagaaatta gagaactggt
     1321 ttcggggctt acccaaagtg caaccggcaa agatcctgaa atcaccatca cgacctggga
     1381 ggaatggaat agcaacagaa aataaatggt ttttgggcaa taatcagtct gtggtgtgcg
     1441 ttagctcgtg tttttacacc gcattcttgc gctaacctta tgatctggca gacaacatgg
     1501 gagagacatc atgtggcagg caatcagtcg tcttttgagc gagcagttag gtgaaggcga
     1561 aatcgaactg cgtaatgaac tgcctggcgg agaagtccat gccgcatggc atttgcgcta
     1621 tgcaggacat gactttttcg tcaaatgtga tgaaagggaa ctgcttcccg gttttaccgc
     1681 cgaagccgac caactggagt tactgtcgcg tagtaaaacc gtcaccgtgc ctaaggtttg
     1741 ggcagttggc gccgaccgtg actacagttt tctggtgatg gattatctcc cacctcgtcc
     1801 gctggatgcg catagcgcat ttattcttgg tcagcaaatt gcgcgtttac accaatggag
     1861 cgaccaacca caatttggac tcgatttcga taacgcgctc tccacaactc cacagcccaa
     1921 cacctggcaa cgtcgctggt caacgttttt tgctgaacaa cggattggct ggcagttgga
     1981 actggcagca gagaaaggga tcgctttcgg caatatcgac gccatcgtcg agcatattca
     2041 gcagcgtctg gcctcgcatc aaccgcagcc ttctctgttg cacggcgatt tatggtccgg
     2101 caactgtgcg ctgggtccgg atggcccgta cattttcgac ccggcctgct actggggtga
     2161 ccgagagtgc gacctggcga tgttaccgct gcatactgaa cagccgccac aaatctatga
     2221 cggctatcag tcagtatccc cgctacctgc cgatttcctt gaacgtcaac cggtttacca
     2281 actctacacg ctgttaaatc gtgcaaggtt atttggcggt cagcatttgg ttattgctca
     2341 gcagtcattg gatagattat tagcagcatg atatgggttg aggataatgg ccgctccgtg
     2401 cggccttttg attaaataaa cccaagcaga gaaaagaaca cataacctgc agcaatacaa
     2461 ataaccggca ggatatacag tgagaaaaac tgcaggaaaa tagtatggtg cggaacaaca
     2521 atacgggact caatttgttc acgcgttagc ccctcttccc ctttggcttt ttccagaatg
     2581 agttgatctt caacaccttc ccgcaagaag cgcgcctggc gactcatccg ggcaccggaa
     2641 tcctgcaacg ccagtccgac aaaaatcagg atgaaaatca cccagaacat aacgttcacg
     2701 ccaccattaa aattgggcgt cggggagtta taccagaata gattcaaaaa aggcgtattc
     2761 gcctgcatca tatcgatcat gacatgagta aagtcgagca tgaccgcatt aatgccttcc
     2821 tggttttcct gccgggtatt cataaatttc agcaaagaaa tcaatgtaga gatcaatgca
     2881 ggtataaaaa tcacccaacc caaaatcctt ttcaaaacag caatgcgtcc agcttgttga
     2941 tacgtcatga gttctccttg attaagacgc gtcgtttcac ttagtttacc tgtagatatc
     3001 tgttttcgcc cattctttaa aggcgatatg ataggcgctt aatcataagc acggcttaat
     3061 accttacaca taatgctcta aaggagaggt tgtaatgtca accccgcgtc agattcttgc
     3121 tgcaattttt gatatggatg gattacttat cgactcagaa cctttatggg atcgagccga
     3181 actggatgtg atggcaagcc tgggggtgga tatctcccgt cgtaacgagc tgccggacac
     3241 cttaggttta cgcatcgata tggtggtcga tctttggtac gcccggcaac cgtggaatgg
     3301 gccaagccgt caagaagtag tagaacgggt tattgcccgt gccatttcac tggttgaaga
     3361 gacacgtcca ttattaccag gcgtgcgcga agccgttgcg ttatgcaaag aacaaggttt
     3421 attggtggga ctggcctccg cgtcaccact acatatgctg gaaaaagtgt tgaccatgtt
     3481 tgacttacgc gacagtttcg atgccctcgc ctcggccgaa aaactgcctt acagcaagcc
     3541 gcatccgcaa gtatatctcg actgcgcagc aaaactgggc gttgacccac tgacctgcgt
     3601 agcgctggag gattcggtaa atggcatgat cgcctctaaa gcagcccgca tgcgttccat
     3661 cgtcgttcct gcgccagaag cgcaaaatga tccacgtttt gtattagcaa acgtcaaact
     3721 ctcatcgctg acagaactca ccgcaaaaga ccttctcggt taatgaccag gggcagtgat
     3781 ctcgctgccc ctggttcttt atctgaattt gcattcaact gacggattaa tcgtcaattt
     3841 aagagaaaga gttacaccgt caccacttcc gtgcactgta taaaaatcct atactgtacg
     3901 tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt caccttctct tttctattgc
     3961 ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg ctggcacagg gtgactggtg
     4021 gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg gatatcgatc acccaaagtc
     4081 gtttcttggg cagcgattaa agtggatatc aaaaccgatc gcccgcgctt ttgggcatcg
     4141 tggttttacc cacagtctgc tggcagtatt tgcgctgctg gcaacctttt accttaaggt
     4201 tcctgaaagc tggttcattc cggctgatgc gctacaagga atggtgctgg gttatttaag
     4261 ccacatactt gccgatatgc tgacacccgc cggtgttcca ctgctctggc catgccgctg
     4321 gcgtttccgc ttgcctatcc tggttcccca aaagggcaac caactggaac gttttatctg
     4381 catggcatta tttgtctggt cggtatggat gccccattca ttacccgaga acagtgctgt
     4441 tcgttggtca tcgcaaatga tcaatacctt gcagatccag tttcatcggc ttattaagca
     4501 tcaggttgaa tactaaaaag gcaaaaatca cctttctgga ataagcaatt ccatttgaat
     4561 ataagagcca gctcacagtt ctgttaatct tgcgccaaca ctatgactgc tacgcagtga
     4621 tagaaataat aagatcagga gaacggggat gaactttcca ttaattgcga acatcgtggt
     4681 gttcgttgta ctgctgtttg cgctggctca gacccgccat aaacagtgga gtctggcgaa
     4741 aaaagtgctg gtgggtctgg tgatgggtgt ggtttttggc cttgccctgc ataccattta
     4801 tggttctgac agccaggtac ttaaagattc tgtacagtgg tttaacatcg ttggtaacgg
     4861 ctatgttcaa ctgctgcaaa tgatcgttat gccgttagtc ttcgcctcta ttctgagcgc
     4921 ggttgcccgt ctgcataacg catctcagtt aggcaaaatc agttttctga ccatcggtac
     4981 gcttttgttt accacgctga ttgcggcgct ggtcggtgtg ctggtcacca acctgtttgg
     5041 tttgacggct gaaggtctgg ttcagggtgg tgcagaaact gcacgtctga acgccatcga
     5101 aagtaactat gttggtaaag tctctgacct gagcgttccg cagctggtct tgtcctttat
     5161 cccgaaaaac ccgtttgccg atctgaccgg agccaatccg acgtcaatta tcagcgtggt
     5221 aatttttgcc gcattcctcg gtgtagctgc gctgaaactg ctgaaggatg atgcgccgaa
     5281 aggtgaacgc gtcttaaccg ctatcgatac cctgcaaagc tgggtgatga aactggttcg
     5341 cctggtcatg cagttgaccc cttacggcgt tctggcacta atgaccaaag tggttgcagg
     5401 ttcgaacctg caagacatca tcaaactggg aagtttcgtt gtcgcgtcct acctcggtct
     5461 gctgattatg tttgcagtgc atggcattct gctgggcatt aatggcgtga gtccgctgaa
     5521 gtacttccgt aaggtatggc ctgtgctgac gtttgccttt accagccgtt ccagtgctgc
     5581 gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg ggcgttcctg aatccatcgc
     5641 cagtttcgcc gcctctttcg gtgcgaccat tggtcagaac ggctgcgccg gtttgtatcc
     5701 ggcaatgctg gcggtgatgg ttgcgcctac ggttggcatt aacccgctgg acccgatgtg
     5761 gattgcgacg ctggtcggta ttgttaccgt tagctccgca ggcgttgccg gtgtcggtgg
     5821 tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg ggcctgccag taaccctggt
     5881 ggcgctgtta atctccgttg aaccgcttat cgacatgggc cgtacggcgc taaacgttag
     5941 tggctcgatg acagctggca cgctgaccag ccagtggctg aagcaaaccg ataaagccat
     6001 tctggatagc gaagacgacg ccgaactggc acaccgttaa ttcttatgct ggcaacggtc
     6061 cgttttgtat aggggccgtt gccttacttt ttaaatgttc catcgcggcg atgcatactg
     6121 actcatattg aaacagatcg tcagcgaaaa aaatggccaa cttatcaata cattcctggc
     6181 tcaatgaatg tgcctgtagc gagaagctta aaacctcata cccgagacgt tttctaagtg
     6241 aactatctgg aatactcata atatcttcaa gtataccact tc
//
LOCUS       DAZFZC010000180         6145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFZC010000180 DAZFZC010000000
VERSION     DAZFZC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.182"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(29..105)
                     /locus_tag="ACHKXP_004824"
     tRNA            complement(29..105)
                     /locus_tag="ACHKXP_004824"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Arg,seq:tcg)
     gene            complement(115..190)
                     /locus_tag="ACHKXP_004825"
     tRNA            complement(115..190)
                     /locus_tag="ACHKXP_004825"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(155..157),aa:Ile,seq:cat)
     gene            complement(219..932)
                     /locus_tag="ACHKXP_004826"
     CDS             complement(219..932)
                     /locus_tag="ACHKXP_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7649648.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1067..1264)
                     /locus_tag="ACHKXP_004827"
     CDS             complement(1067..1264)
                     /locus_tag="ACHKXP_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7649649.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(1506..2036)
                     /locus_tag="ACHKXP_004828"
     CDS             complement(1506..2036)
                     /locus_tag="ACHKXP_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO7649650.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(2045..2404)
                     /locus_tag="ACHKXP_004829"
     CDS             complement(2045..2404)
                     /locus_tag="ACHKXP_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7649651.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(2417..3466)
                     /locus_tag="ACHKXP_004830"
     CDS             complement(2417..3466)
                     /locus_tag="ACHKXP_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7649652.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKIRSVYRESDIVPGEQTATSILKQR
                     TKNIALPPHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(3468..3746)
                     /locus_tag="ACHKXP_004831"
     CDS             complement(3468..3746)
                     /locus_tag="ACHKXP_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649653.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(3914..4069)
                     /gene="hokD"
                     /locus_tag="ACHKXP_004832"
     CDS             complement(3914..4069)
                     /gene="hokD"
                     /locus_tag="ACHKXP_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7649654.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            complement(4315..4419)
                     /locus_tag="ACHKXP_004833"
     CDS             complement(4315..4419)
                     /locus_tag="ACHKXP_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649655.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            complement(4535..4873)
                     /locus_tag="ACHKXP_004834"
     CDS             complement(4535..4873)
                     /locus_tag="ACHKXP_004834"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7649656.1"
                     /translation="MKKKDRDADLRWIHGVIVQAAWFVKASLEQNALSGNYPVTPDSW
                     ISCSERMPDTKTAVLVAVEFDRKGDWRMKWATYIPGHPDANDGWIIPGASWKPSHWMP
                     LPEPPQEVRQ"
     gene            complement(4973..5068)
                     /locus_tag="ACHKXP_004835"
                     /pseudo
     CDS             complement(4973..5068)
                     /locus_tag="ACHKXP_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5279..5404)
                     /locus_tag="ACHKXP_004836"
                     /pseudo
     CDS             complement(5279..5404)
                     /locus_tag="ACHKXP_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5415..5678)
                     /locus_tag="ACHKXP_004837"
     CDS             complement(5415..5678)
                     /locus_tag="ACHKXP_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649657.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            complement(5680..5898)
                     /locus_tag="ACHKXP_004838"
     CDS             complement(5680..5898)
                     /locus_tag="ACHKXP_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO7649658.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            complement(5931..6143)
                     /locus_tag="ACHKXP_004839"
     CDS             complement(5931..6143)
                     /locus_tag="ACHKXP_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649659.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDGNVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
BASE COUNT         1544 a         1632 c         1343 g         1626 t
ORIGIN      
        1 ctgagctaac aacgcatgat gctgataatg gaccgccatc ggggacttga accccgcgca
       61 gccagcttcg aaggctggcg ctctgtcccg atgagctaat ggcggtatgt gatatggtgg
      121 cccttgctgg atttgaacca gcgacctggc tattatgagt cgctcgctct caccactgag
      181 ctaaagggcc gagccaaaaa ataataatca gatgaaaatc aataatcaag cccttgcctg
      241 gatacatatc tgtctggcgg gaagccataa tagcggtgaa atacagaaat aaagtaggac
      301 ctgcttgaat aaccgcattt ttctgctata gcctgtccat atccatgccg ggaacataac
      361 atatttacag caacacgcat ccgctcttcc agcaacaagc gactgaacat gcccccttca
      421 tttttcagtt ttgtctttaa caaactctca ctcatatgca actgtagagc aatcgcacca
      481 agcgtccagc ttgctgatat atctgtctga attatcgccc tgactttggc acttatgctg
      541 gataaacatc cacttaaaaa caatgacatc cgttcatctg tttcaaacag agacaggcag
      601 gccatcataa gaaacatatc cgtggcctct ccggaaaatc cctggctggt aattaaagcc
      661 gcagccaacg caggattgtt gggttccagc aacaggtaaa gcggaatgtc agtcagacga
      721 gtcctcgtca gcttatgctg actttccaga tattgactta cgacggattc gcttatatcg
      781 acaattttaa ctttgccata atgcataagg aaaagctccc tgatgcattt ggtggccaga
      841 acgactgagc ctggcttaag tgacaacgta tccttttcaa gaaaaatatt aattggggag
      901 caaaccatga taactgaaca gacaacagcc attataattt tactttcatt agcaattggt
      961 tagctcaatt atagccccaa aaggtaaatt atcatcaaca cataagcaaa ggactgacag
     1021 gtgtcgcccc cccaccagcc gcccattcac cacaaataaa aagccttcag gactgaaggc
     1081 gtctgtaaca accgcactga tagtctgcca gacccgccat aacaagctgg gtcagtatta
     1141 actggcagcg ttcgcgtgaa aggtaagtat tctgcgcaac ctccccgact gtcgccggtt
     1201 cggtaacgct taattcatta aacaccactc tggcggtttc ggtcatatcc tgctgtttca
     1261 gcatgtcttt ttcccttttt cggttaacgt gacacaccaa taactcttgt cgaaaaagcc
     1321 agcaagctga aagaacggta ttaataacca ccagcgaatt tattgcgctg ctgtatatta
     1381 cggacacaaa aaaaccacct tccggtggct tccttgtgcg aaaaaacttg catttcgcct
     1441 cgcgatacag ctttgcgaag cttacaggaa ttcaagctgt ttctgcgtaa aaaagcaagc
     1501 tttttttatc gaaatgaatc gtgcataggt acataaagca tgtgttcagc cacggctaac
     1561 caacctgcaa tacgtttctc acatgtgctg aaacaccatt ccgggtgagt acgatttaaa
     1621 cattctgcca tttttctctt actcattccc cgtccttcgt acctttgccg gagaatattg
     1681 attagcccgg gatattcccc aagcacctca ctgataacgc gatcaataat caacgcctct
     1741 gtgtctgtac aatgtgacaa ccagctcttc tgcttccctc tggtcatatc ccgaaaaaat
     1801 gcctcaagtt ccggtttttc cagcccggat ttcttcatgc tgcgtaaaac ctcattaact
     1861 gctgttttcg tcagcttttt cgaaaccagt aaccggttaa acatatttcc ggatttaccc
     1921 ccaccgatat acgaccaccg cccccacatc cgtaatttcc cctggatcca gactgcttcc
     1981 agcgtgttca ggcgtaaatg ttcgccgctt ttgcctgtaa tttccggata tatcatattt
     2041 acgctcactc actctcaatt ttgtaaatct tcacacccag ccgtccacca gatactggct
     2101 gaccgcgcac aatattgatt tcatcaaact gctcgtcatc aatgagcact tccgcatgcg
     2161 tcagcgcatc cagcggtgct ttcagaatgt tgtccaggtc acggcggcgc ttatccggtg
     2221 gttctgcaat aatttttatt gccagccgtc cggacaggct taatttcagc cgctgctggc
     2281 gaacaataag cgccacagcc cggcgataac gctcaccggc ttttgataca aaatatgtgc
     2341 tgccacgacg tcgccagtag gtgttcaccg tcggcgggta aggcaaaaca aactctatac
     2401 gcatcagtaa cctcttttac ccgagcacgc cggttgcaaa agcgtgatca agaaaacgaa
     2461 aaattaaatc aacctgggaa ccatgctttt cttcgaacgc cagcggatcc gcatgaagct
     2521 cgttgtgatg ctcccgacac agcggtagcg tgaaaatatc gtgggatttt gttcccattc
     2581 caccctgacc atgaccaatc agatgatgag gatcgtccgc tggcttacca caacacgcac
     2641 acggctgtgt ctttacccag cgtgtgtatt tctcatttac ccagcggcga cgtttaggtc
     2701 gtttcatgaa ggattccgga gactccggat caacggcaat gctgaccacc gtcttttcct
     2761 gtggtgggtt ctgttgctgg tgggtgtgag gcggtagcgc aatatttttt gtgcgctgct
     2821 tcagtatgct ggtggcggtc tgctctcccg gtacgatgtc gctttcacgg tatacggagc
     2881 ggattttttc cgccggtaat cccagagaac gacgcgatac tgcctcaggt aatgcgtcca
     2941 ccacctgatt gcagactgcc caccaggata attcagccag cgataattcc cgttcctgta
     3001 tgccattcat tgcgtggcgg atgacgtcaa tcatccatgc tgacaggttt tgttgagcaa
     3061 gttgctcaag tgattcggat gtctggtcgc gcagctggtt gtcgcagtgc cagcacaaca
     3121 ccatcgcgcc ggtaccgtaa cgatgtatga cagtttcgct gtgatgataa tcaccatgag
     3181 gccactggca ggatttaacg tgacgtaata accagtcaga cagtgcacca gcgccaccag
     3241 cagcacgaat cacacgctca ttgctgaaaa atggcagtaa tgatttatct tccgccagcg
     3301 gctggcgaac ggcaggaacg accccggacg gcagattacg catgcttttc ggttcagact
     3361 ccaccagcac cctgccgcca tgaaataccc gcatggattc acggcctggc ttaacgataa
     3421 ccagaccgag ttccggtacc agaacaggtc gaagtaatac ccgcacatta cctccagatc
     3481 cgttgctgga atgtgcggga cggacgcggt ggccgttcgg agtaagggag cctgacggag
     3541 attatccagt gacggtagtc gaggctaagg gcttttttaa cctcgcatcc gcgcctgcgg
     3601 taacactgaa tgagccattc ggcctgttct tcagtgcatg ggggatgctg gtaccagtca
     3661 gatttgaatg cgtgaaaaca ccgtccgcgc ctgctggcaa agacggcaga atcatcagaa
     3721 ttgtataatt tggtatcgtg cgccatcggt tgtctctgct ggcgcagcag gtgccagttg
     3781 ttcaggctgg cgtgcgaatt gtaaaccaga atgccaggaa aaaacaaaac ccgccgaagc
     3841 gggttaagtg cgggtgcgtt gaggatgcct gattcatcag aggtggcgag ggatttctcc
     3901 cccgccgggg ctcttactcc tcaggttcgt aagctgtgaa gacagcgacc tccgtctggc
     3961 cggttcggac tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     4021 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     4081 ccttgccttt cggcacgtaa gaggctaacc tacgtttgtg aagcatagat tgggcctcag
     4141 attaatgtta agcgtcttgc aggacgcgta atgttaactg gggcttttct ctgtctgcct
     4201 tacggtggca tgcccgaggc agacagcctc aagcacccgc agcaattcta cttaactctc
     4261 gctttaccgc aaaccgtttt tacccgatat gggaattccc atatcgtaat gaattcagtt
     4321 ccctagtcga tccatcaaaa acacaaccag gcagtaaacg cccacaacag caacaacagc
     4381 cagcgcacct tccattgcca gtgatatatc atccgacata ttccctcctt tggtgttaat
     4441 cccggcgaac gtttttaccc ccaccgacaa ataacatata ctaaaaaagc gatagccata
     4501 gcaacgcctg taattgcaaa tgcttcaggc cagttcattg gcgcacctcc tgcggcggtt
     4561 ctggtagcgg catccagtgt gacggtttcc acgacgcacc aggaattatc cacccatcat
     4621 tagcgtcagg atgacccggg atgtaagtcg cccatttcat tcgccagtca cctttcctgt
     4681 caaactccac ggcaacaaga acggctgttt tggtatccgg cattcgctca ctacagctta
     4741 tccaactatc cggagttacc ggatagttgc ccgatagtgc attctgctcc agtgatgctt
     4801 ttacaaacca cgctgcctga actataacgc catgaatcca gcgcaaatca gcatcgcgat
     4861 ctttcttttt catcttttcg ccacttaagg ccttgcttat gtggctgcgt accaggtctt
     4921 catgtaattc cttcgcctcc tcaatggtga aaccaccagg cagaagagcc ggagttaccg
     4981 gagagctggt tgacgcttcc gggattttcc gaaaattatt ggttgacgaa tctttatttt
     5041 cccgaaagtt tccggactga agcatggctt cgcggcaatc gttccagcct gtagcgtatg
     5101 cagccgcttt gctgctgcct tcaactggcg catcctgcca atacatttct tccggcacta
     5161 tcggcgctgg aggggcggca aatagatatc cgccaaagtc aggaagctct ctaatggcct
     5221 gtacgaattt ttgtttgcct acgtcaaccc ctaatgggta atgagctata atctttgcca
     5281 ccggctctgc ttccagcgat gccagcgcaa tccgtgccag ttccatttgt tcaccacggg
     5341 taagcccgtt ttcaaccgga tttttaatga acaattcaat acgttctttg gtaatagtgg
     5401 tcatgtgtta ctccttaacc cgcagtgctt tcaactgatg aggggaacaa aatcttttca
     5461 tcaaaccctg cattcatatc atgaacagca acacaccaat ccatcgacga acgattatca
     5521 agagcctcca tgatttcatc catgcggcgt aggtcataca ggtaaatgct tttatcgcca
     5581 atggtgtaaa agccaatttt tttcggtgat ggacagcgat caagaacttc ctgtaattcg
     5641 tttaaccatg cccgttcttt ttttgtcaaa gttgccatat cagttttcct tatacggatt
     5701 aattttattg tgcagtgtgt tgaacgacgc ccatacaacg tcggtataca attcagtaac
     5761 tggctcaatt attttcccga ttgcccagac aaaaattaga ggggatatcg gtatcatcaa
     5821 tacgataaac agaatgagaa acaaaaattc tgtcgcccta cttttttgcg gatattcttt
     5881 tctgaataat gtagtcattt cttaccgccc tttcgggcgg cctcccgaca ttaatcgttg
     5941 tggtaactca tggcttcatt tgcagcatca accggatcaa cctcccacca gcaataattt
     6001 ggtgcgtttc cttcaggtgt ccacggttct aattcatttt ttgccacatt cccatcgcca
     6061 gtaattttaa aaatctgctc agagaatttt cttgcccact cgttatattt ttccgcatta
     6121 atggctttct gtgtatttaa cataa
//
LOCUS       DAZFZC010000181         6086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFZC010000181 DAZFZC010000000
VERSION     DAZFZC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.183"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(33..875)
                     /locus_tag="ACHKXP_004840"
     CDS             complement(33..875)
                     /locus_tag="ACHKXP_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7649660.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSVSHILAVRKSTLLQIDTLIRQ
                     LAEISVMTESIGGKTALDWAMKQDFRCGCWLMEKTETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(960..1157)
                     /locus_tag="ACHKXP_004841"
     CDS             complement(960..1157)
                     /locus_tag="ACHKXP_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7649661.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLPDQNR"
     gene            complement(1169..1657)
                     /locus_tag="ACHKXP_004842"
     CDS             complement(1169..1657)
                     /locus_tag="ACHKXP_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7649662.1"
                     /translation="MNLSLTLEADSLNVQALNMGRIVVDVDGIELAELINNVTENGYS
                     VRVVEDSGQQSTCTPPPLTILAGIRCSTAHITETDNAWLYSLSHQSSNFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(1654..2031)
                     /locus_tag="ACHKXP_004843"
     CDS             complement(1654..2031)
                     /locus_tag="ACHKXP_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7649663.1"
                     /translation="MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRSHYRTVNDITLGKRTEAKR"
     gene            complement(2078..2452)
                     /locus_tag="ACHKXP_004844"
     CDS             complement(2078..2452)
                     /locus_tag="ACHKXP_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7649664.1"
                     /translation="MSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            complement(2527..2748)
                     /locus_tag="ACHKXP_004845"
     CDS             complement(2527..2748)
                     /locus_tag="ACHKXP_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7649665.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            complement(2817..3293)
                     /gene="radC"
                     /locus_tag="ACHKXP_004846"
     CDS             complement(2817..3293)
                     /gene="radC"
                     /locus_tag="ACHKXP_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7649666.1"
                     /translation="MQQLSFLPGEMTPGERSLIHRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPGKTDRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(3309..3782)
                     /locus_tag="ACHKXP_004847"
     CDS             complement(3309..3782)
                     /locus_tag="ACHKXP_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7649667.1"
                     /translation="MKTLSQNTTSSVSAPETDLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPESAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(3920..4120)
                     /locus_tag="ACHKXP_004848"
     CDS             complement(3920..4120)
                     /locus_tag="ACHKXP_004848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649668.1"
                     /translation="MKHIPEGMIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            complement(4120..4941)
                     /locus_tag="ACHKXP_004849"
     CDS             complement(4120..4941)
                     /locus_tag="ACHKXP_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7649669.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(5042..5250)
                     /locus_tag="ACHKXP_004850"
                     /pseudo
     CDS             complement(5042..5250)
                     /locus_tag="ACHKXP_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            complement(5351..5806)
                     /locus_tag="ACHKXP_004851"
     CDS             complement(5351..5806)
                     /locus_tag="ACHKXP_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO7649670.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFME
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(5882..>6086)
                     /locus_tag="ACHKXP_004852"
     CDS             complement(5882..>6086)
                     /locus_tag="ACHKXP_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649671.1"
                     /translation="NLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVME
                     YDASHLGNMVNRLEEALPPELPA"
BASE COUNT         1382 a         1548 c         1651 g         1505 t
ORIGIN      
        1 cttttgatat cgaaagggaa tcacactcgg gatcaggctg agagcacaga aggaaagtac
       61 ctggctatta tatcgttaat tctgtcaatc agttccagcc ttttgaaagt gatgtgtgca
      121 gatcccttct taaagtattt aatgatgaac atctcgtctt catagcgctc tttaccctga
      181 acggactgga tgtgaccatc cagacgaata gtgatgtttt cccggttgtc ggggatcggt
      241 ttgccgctga acagatgtag cattctttcc aggtcgacga gtcggtcagt ctgttgcccg
      301 gtgataagat gaaaccccca ccggtcccac ctcactaggt tgttgacgat aattttactg
      361 ccaaatttac aggggcaatt ggttttgtaa ttccagctca gccctctgaa gaggttgatc
      421 acacctcgct caaatacttc gtccttattc tggtgcagtt gttcaaacgt actcaggata
      481 ttcgcctcgc tgatttccgg aaaattatcg tactccagtg atcggtacca tgtatcacgt
      541 gcctgtgcat ccattaatga cagcatccct gaacgttgca tcaggtcacg ccagatttcg
      601 cggtcaaggt tgcgggtgat agctttcatt gcggtttccg ttttctccat cagccagcag
      661 ccgcagcgaa aatcctgttt catggcccag tccagtgcag ttttaccgcc aatgctttcc
      721 gtcataactg agatttcagc cagttgccgg ataagtgtgt cgatttgcag cagtgtactc
      781 tttcgtacag ccagaatgtg gctgactgag gttgagcaaa taacgtccgt atgcccgttg
      841 accacttccg gacgggaggc tgtgatatca gacataatcg atattccttt ttacagataa
      901 acgaaaacgc cagccgtatt caggctggcg tcagtggaat gaagagtggt gaatgagact
      961 caccggttct ggtctggaag ctgcagctgg cgcagggtac ggatatcctc cctggcctgt
     1021 tcaatacagg gcaacagtgc tgccgaatcc gttttatctt catcgttgtc aaagataatt
     1081 cccgattcgc agtcgatatt gtcctgcagc catgcaatca gaatatccag cgctgtttcc
     1141 gtggttaatg atttcatgtt gtttctggtt accagtcgaa agtgggtaaa tccggcagac
     1201 attcggcgct ggcatccaga tgaatgagac tgacgccata acgtcgggta agtgtgacaa
     1261 ccagacggcg gaacgttctg gacaggccca ggcgtttaag ccgcagaacc gggtatgacc
     1321 acgcatcagt acgtaacaga tatccgctac ctgtaaaatg aatccattct gattcgccga
     1381 agttactgct ctgatgcgac agcgagtaca gccaggcgtt gtctgtttcc gtgatatgtg
     1441 cggtgctgca gcgtatgccg gcaaggattg ttaacggtgg tggtgtgcag gttgactgtt
     1501 gcccggagtc ctccaccacg cggactgaat aaccgttttc agtgacattg ttaatcagtt
     1561 cagcgagctc aataccatcg acgtcaacga caatgcgtcc catgttcagt gcctgtacgt
     1621 taaggctgtc ggcttcgagc gtcagggaaa ggttcatcgt tttgcctccg tgcgtttacc
     1681 cagggtaatg tcatttaccg ttctgtagtg gctgcgggtc atcaggccgg ttgcccggcg
     1741 ggcccggaga atatcaatgc tgttgattaa ctgcgacggg gctcctgcgc tgaatcccgg
     1801 ctggtcagtt cgtaccagcg cgtatttttc aacgagaaag ttcaccgcat cacacagtga
     1861 aatccctgcc tcaatatgct gctcaatcac acgttcatca gcgaacggtg tgtcattcag
     1921 cgtgaggcca tagtgctggt ccagcagtcg ggtgagcagt gtctgccaga tggtgacggg
     1981 agacgggcag cccgatgcct cccgtacgtg tgtgtcaggt aatgttttca tcttaaattc
     2041 tctgtaactg gtagtgctaa aggtgagata ttcggggtta tgaggtggtt gcgggtgcca
     2101 gtgtcggata aacagccata taaacgtaac cacagctgcc gagggtgtcg gcttcgcagg
     2161 tcagccctct ggcatacagc gtgacggtat gctgatggcg gggattcagt tcaccgctgg
     2221 tgagcatgag ttccagttgt ttcatcagca gcggaaaggc ctggtccaga tgatacgcat
     2281 ccgcgtcgct aaactgcccc ctgataccgg cgcggtcggc aaggtaatgt aattgattgc
     2341 cctcctgcac cagacgtgcc ccgaaacagg gcgtcacggt gcaggggagc ccccaccagg
     2401 ggcggtcgtt gttgtcgttg ggataatttg tctcatggag tgtgcctgac acgataaaat
     2461 cctcacaaaa aatcagttaa gaatggtccg gtggcagcga taattctgct catcatttct
     2521 cctgactcag ttcagtgtga cgctcatcag ccggacatac gagccaaaac tgtccttacg
     2581 gcgttctgca aacacggcaa gaacgccggg aatatcctgt acttcgcgac cggtatacgc
     2641 ctcagcgcta ccgtgccagc gatacttacc ggtacagaac ggaaaaagac gggatgcagg
     2701 atgctgttgg tgaatacgca tggcttcacc acgggtgatg attctcatga tgtgatacct
     2761 ctgaagacag aagataaaag cgaaaacagg tatgatgtgg tggtgacggt gacgggttaa
     2821 agcagaccgt gttccgcaaa ggagaaaacc tggctgccac cgactatcag atggtccggc
     2881 acccggatgt caaccagggc cagcgcctgt accagacgtt cagtgataag ccggtcagtc
     2941 ttaccaggtg tgacttcacc ggacgggtga ttgtgtgcca gcaccacggc agcggcattg
     3001 tggtacaggg cgcgtttaat cacttcccgg ggatggactt ccgtgcggtt gatggtgccg
     3061 gtgaagaggg tttcaccggc aatcagctga ttctggttgt tcagatacag cacccggaat
     3121 tcttcacgct ccagtcctgc catgttcaga atcagccatt cccgtgccgc acgggtggag
     3181 gtgaaggcca cgccgggttc atgaagatgg cggtccaggg ttttcagggc ccgatgaatg
     3241 aggctgcgct cgccgggcgt catctctccg ggcagaaagg aaagctgctg cattgtccct
     3301 gcctccattc agtcgatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa
     3361 tcccgcagcc ggtaataatg gaccgtcatg gcataattct ccgtacgaca ggcatgatga
     3421 ctgtacgtca tcagacaggc ggcaataccg gcggattccg ggctcatttc agcgcggtta
     3481 ccgttcatgg cattgaacag cacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc
     3541 ataaatgcgc caccgttgtt cagggtgtac agattccaga taccaccgca gtagtcttcg
     3601 caaagacggt ccatccagcc gaagatacgg ggctccaggg tcacccactg tggaatgagg
     3661 ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag ggactatggt ggcaaccagt
     3721 tgttgcaggt cagtctccgg tgctgagacc gaagaggtgg tgttctgaga caatgttttc
     3781 atgtatgaaa tccttgtaat gtgaataaat gagagggagt aaaggccggg ggctgacaac
     3841 atcacgttca tcactgaccg tgagacccgg taatgtcagg gatgaatgat attcggctga
     3901 aattccggac agccgtacgc taaccgttcc ggtcacagac ggatggccag atgccattgc
     3961 ctgtactcag tctgcacaga ctgagttctc cgggacggta aagcagacgg gtacaccagg
     4021 cgcacaggtc atcatcagaa atggaacgac accccggcag cgcctccggg gcgtcaggtg
     4081 attctggtac ctgaatcatg ccttcaggga tatgtttcat cagcgcatac tctccagcag
     4141 cgtttctgcc attacccaca atgcgcggtt gagcttaata tcggtgtcga tgctgtgaat
     4201 ggcacgggtg tggatacgtt ttcctctggc actgcgaccg gaaatccccc ctttcagcat
     4261 attctcctgg atggtctgat aagcactcca caggtccttt ccgtaatcct cccggcgtcg
     4321 tggcgtcaga atgtcggcgg tggtgacggg ctgatgttcg tcaccataac ggtaagtcag
     4381 tgccgcctgt gccagcgcct ggcgtgccgg tggcggcagg accagcgact gcatggcatc
     4441 acgcttctcc tctatccggt caaaaacccc caccacctcg taagcccctt cgataacttt
     4501 ctccactaca tttccccggt gcggaacacg cacttccccc agagactgac cacagacgca
     4561 cccgttctgg cagacgaacc tgaagtaacc cggcagcatc tggtagctgg aggtaccgtc
     4621 atgagagttg agcagaataa tttcagggac atgttctccg tttatctctc cggcccgccg
     4681 cagacgcagc atgtgttttg tgtatccccg acggcccggg tcgcgcacac gggtctggca
     4741 ggcgaagaat ggctggaagc cttcccgctg cagactttcc agcacggtga tggtggggat
     4801 gtacgtatag ttctggcttc gggacgtgtg tttatcttcc ccgaaaatcc ccggcacatg
     4861 gtgcatcagc tcttcatgtg tcagtggacg gtcacggcgt atctggtttg cagcataacc
     4921 aaaacgactg gctaatcgca tactttgctc cttatcggtg gttaagattt actggtgtaa
     4981 taaatgaaaa aagccgcgtc tcccggagaa gacgcggcct gatggacgaa atcaatgacg
     5041 tttattgtct gagaagccct tcactggcga gccacgcatt aagctgcttt ccggcacagg
     5101 gttcagtatt ccggcagcgc cagcgtcgct gaccttccgc gttgcggata accagccgga
     5161 agtgcgcacc tggtcatctt caataagcac attaaggtaa gctgttgtga cagccaccgt
     5221 ctgcccgtat gagaaagatc cttcagccag gacatactct gtgtgtaacc cggcatatct
     5281 ggtttctcct gataaatagc ctctgccata cgttgtggca gaggctgaag catgaaactg
     5341 acttcaggga tcagttaaca ttttttccgg aaacggtaat cagcagtgga tggtagtcct
     5401 ggggatcgaa aaccgataac ggcagactga cacgatggcc gttactttct tcagttgctt
     5461 taatgatttc ggttgtggcg acattttcca cgcactccgt ttccataaat gcgtctgtgg
     5521 ttcgcgtggc attactgtca ccaaaggctt ccgtttccat ttttctggtc accagcgtct
     5581 gaccatattt gtctttgagt tgcagagtga tggtgagggg gccaaatcct tcatcgtttc
     5641 cgccattatc cagccggaac tggtaggcac aaatatttcc cgggagccat atcgtatctg
     5701 tattgcgtat actgatgtaa cgttgatcct gtgcccggag tggggcagac cacgttaacc
     5761 ccagaatgaa ggcggtaatc atgcaggttt tgaacaggtg aatcatggta tttacctctc
     5821 tgagtcatga cgatgacact gacaaatcag gtgataaaac gtaaaaggcg cagaatagcc
     5881 gttatgccgg taactccggg ggtaatgctt cttccagtcg gttaaccata ttgccgagat
     5941 gggatgcatc atattccatg acggggcgtt gcctgatgat actgaccacc agtggtttga
     6001 ttaacatatt ggtcgcggcc cgttgttgta taccggcggc gaaaatgatc tccagagcat
     6061 tctgatcgat aacacgggcc agattc
//
LOCUS       DAZFZC010000182         6051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFZC010000182 DAZFZC010000000
VERSION     DAZFZC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.184"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(78..839)
                     /gene="kduD"
                     /locus_tag="ACHKXP_004853"
     CDS             complement(78..839)
                     /gene="kduD"
                     /locus_tag="ACHKXP_004853"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO7649672.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(869..1705)
                     /gene="kduI"
                     /locus_tag="ACHKXP_004854"
     CDS             complement(869..1705)
                     /gene="kduI"
                     /locus_tag="ACHKXP_004854"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO7649673.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASDDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1992..3173)
                     /gene="yqeF"
                     /locus_tag="ACHKXP_004855"
     CDS             complement(1992..3173)
                     /gene="yqeF"
                     /locus_tag="ACHKXP_004855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO7649674.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3428..4657
                     /gene="yqeG"
                     /locus_tag="ACHKXP_004856"
     CDS             3428..4657
                     /gene="yqeG"
                     /locus_tag="ACHKXP_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7649675.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGYLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5117..5749
                     /gene="yqeH"
                     /locus_tag="ACHKXP_004857"
     CDS             5117..5749
                     /gene="yqeH"
                     /locus_tag="ACHKXP_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7649676.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFFRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         1685 a         1447 c         1266 g         1653 t
ORIGIN      
        1 catgatatgg attatgatga tctacaggta taaaaaaccc tgccatgcgg cagggtcata
       61 aaagtaagaa gaatgaatta acgcgccagc caaccgccat ccacggcaat ggtataacca
      121 ttcacataat ctgaagcgct ggaggcaagg aacactaccg gccccatcag gtcactcggc
      181 agtccccaac ggccagctgg aatgcggtcg agaatttccg cgctacgttg ttcatctgcc
      241 cgcagttgtt gagtattgtt ggtcgccatg taacccggag ctatcgcatt aacattaatg
      301 ttgtgtttag cccattcgtt cgccatcaat cgcgtcacgc ccatcacacc gctttttgat
      361 gcggtataag aaggcacacg gatcccgccc tggaaggaga gcattgacgc gatattgata
      421 atcttgccgc cattgccttg cgcgataaag tgtttcgccg ctgcctgaga catgaagaat
      481 acgctcttga tattcaggtt catgacatcg tcccagtcct tttcgctgaa ctcgagagcg
      541 tcttcgcggc gaatcaatcc ggcgttattc accaggatat caatatgacc aaactccgct
      601 accgcgcgat ccagcagtgc tggaatacca tcaatctttc gcagatcggc ggtcaggctt
      661 aaaaaacgac gccccagcgc tgtgacctgc ttgatggttt cagtcggttc aacgatgtta
      721 atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg ccatcccctg gcccagtcca
      781 gtatcacaac cagtgacgac cgcaacttta ccttcgagag aaaatgcact taaaatcata
      841 acaatacctt attcgtttat gccgacaact agcgcaaatc tttaacggcc acatggtcca
      901 tatcatcaaa gacctggttt tcaccgacca tgccccagat aaaggtataa gctttggttc
      961 cgacgccgga atgaatcgac cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa
     1021 tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac gcaggcgtca tcatccatat
     1081 tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg acacggcatg gtgttccaca
     1141 agttacccgg agccagctcc gtcagcccca tactcaattg gcaggtttcc agcacatccg
     1201 ggacaaaata tttgttaata gtgcgacggt tactggtgag gttatcgcct aacgtgactg
     1261 gagatacttc gtccggggtg acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat
     1321 tgtaataaaa cttcgccgga gtggcggtat catcactggc aaagacaact tcctttgcac
     1381 ctttaccaac atacagggcg tcgcggtgac cgatttcata gcattgacca tcgacagtaa
     1441 tcgtaccggc accgccaata ttgataacac ctaactcgcg acgttcaagg aaataactta
     1501 cgccgagttg tttacccact tccccgccaa cggaaaccgt tttagttacc ggcataatgc
     1561 cgccaacaat aatacggtca atgtggctgt aaaccatggt gtactcatcg gcgacaaata
     1621 cctcttcaac caaaaattca ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac
     1681 tgtggatgct ctgtcttacg tccacatcaa cctccgataa tcagtgaagc gtgaaaatag
     1741 aaaacgatag tgaaacactg tttcgttttc tctataaagg gatgatatgc ccatgggcta
     1801 gggttttcaa ttaaaaataa aacaatgttt catttttaat ttaaggatta aaaaaagtgc
     1861 agtgtcgatc acgaatatgt catgagaatg gaaaaatgag ggagcccaga aaatgtatgt
     1921 cgacttgcca gccatcatta ttcaacagat taatgacggc agatatttat cggtaaggag
     1981 gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca cgccctggcc cccgccgata
     2041 caaagcgttg ccagcccttt gcgggcatta cgtttcacca tttcatgaac cagagaaacc
     2101 aggattcggc aaccggaagc gcctatcggg tgaccgagtg cgatcgcgcc accattgaca
     2161 ttgacccgac gctcatccca ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac
     2221 gcttcattag cctcgataag atcgacatca gccaactgcc agcctacacg ctccaggcaa
     2281 cggcgggtcg catacaccgg cgcaattccc atcaatgccg gatctacacc aacgctggca
     2341 aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc
     2401 atcattaccg cagctgcgcc atcgtttatg gatgatgcat tccccgcggt cacagaaccg
     2461 agactatcaa atgaaggatt taaacgggct aaaccttctg cactggcgtc agtgcgtggc
     2521 tgttcatcgg tatcaacaac caacgtctga ccgttacttt ggatcattac cgggacgatc
     2581 tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg
     2641 taagcatcct gcaactgacg gctgatgcca tattcgcgag ccagattttc ggcggtgaca
     2701 ccaatatgat aatcattgaa ggcatcccac aacccatcat gcaccagact gtcaaccaac
     2761 tggctattgc caagctgtgc accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc
     2821 atgttttcct ggccaccggc gatgacaata tcagcctcgc cacactgtat cgcctgagta
     2881 gccagatgca gtgctttaag cccggaaccg caaacgtcat taatagtgat tgcagaaacg
     2941 ctattaggaa gaccaccttt aatagccgat tgccttgccg gattctgccc tgcccctgca
     3001 gtcaacacct gaccaagaat tacttcatcc accgcatatg caggaacgcc ggtacgttct
     3061 attaacgctt tcacgaccag actaccaagt tccacggcgg aatgacccgc taacgcaccg
     3121 cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca caacgtcttt catcacaacc
     3181 tcgcaccaat taacagtgca atagtaaatg attgttaatt acagttatct taattgttta
     3241 aaaaaagtga tttttatcac aaaggaaata tgcctgagca gtagtcagag tcataactgg
     3301 cacgtaaggt ttgcaaccac taacccacca atagaggggt agatagggcg ttaatctccc
     3361 atacttaacc tggtttatgg taaattgccc tccattttgt ttaatttgta gatgatacgt
     3421 tcagataatg tctaatattt ggtctaaaga agaaactctg tggagtttcg cgctctacgg
     3481 cacagccgtt ggtgcaggca cgctcttcct tcctattcag ttaggttcgg caggggctgt
     3541 ggtcctgttt attactgctc tggtcgcctg gcctttaaca tattggccac ataaagcctt
     3601 atgccagttc atcctctcat cgaaaacatc agcaggtgaa gggataacgg gcgcagtaac
     3661 acactactat ggcaagaaga ttggttatct gattaccacg ctgtacttca tcgccttttt
     3721 tgtcgtcgtg ttgatatatg cagtggcaat taccaactca cttacggaac agctggcaaa
     3781 gcatatggtt attgatcttc gcatccgtat gttggtgagt ctgggtgttg tattaatcct
     3841 gaatctcatt tttctgatgg gacgccatgc aaccattcgg gtaatgggtt ttttggtatt
     3901 cccattgatt gcctatttct tatttctttc catttacctt gtcggtagtt ggcaacctga
     3961 tctattaacg acccaggtag agttcaatca gaataccctt caccagatat ggatatcgat
     4021 tcccgtgatg gttttcgcct ttagccatac gcccattatt tctacgtttg ccatagacag
     4081 acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa aaaattatga aagtcgctta
     4141 tctcatcatc tgcataagtg tactgttctt tgtctttagc tgcctgcttt ctattccacc
     4201 ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt ttatcggcgc tttctatgct
     4261 gccgaacgcc ccagcatggc tgtcaatttc cgggattatt gtcgcagtag ttgcgatgtc
     4321 gaaatcattc ctgggtacgt actttggcgt tattgaaggt gccacggagg tcgtcaaaac
     4381 aacactacag caggttggtg taaagaaaag tcgtgcattt aaccgcgcac tatcaattat
     4441 gttggtatcg ctgatcacct tcattgtttg ttgcattaac ccgaacgcga tttcgatgat
     4501 ttacgcgatc agcggcccgc tcattgccat gatacttttc atcatgccta cgttgtcaac
     4561 gtatctgatc ccggcgctta aaccctggcg ttccatcgga aatctgatta cgctgatcgt
     4621 gggtatcctg tgcgtatcgg taatgttctt tagctgatat ccattgtgaa tggttcaaaa
     4681 ttgctacaat tttgaaccat tccagttttc ataatgttca ttcgttatta ttccgcacta
     4741 tcacttccct catcatatcc tagtattatc agttagttaa acacacaaag agatctgaac
     4801 atcttaaata agactattta agatgcataa cttagattcg caagatatct tccggtcatt
     4861 caaaaacaat ttgcgatata gatattctca taccatgaga tagttatctg aaccaccctc
     4921 aaccaggtaa gattaaatca gtgaaacata atcatattta ataacgagaa atgcatttta
     4981 aaatgtctta atcagaatta tagcaaatac agattaatcg caatttttcc cagcaaaatt
     5041 acaaaatcat ggtgtattga catcatcaat caccaatgaa attatgcaat tatatacgga
     5101 tagggaggtt cttaacatgg ggctttgtag tcgttataaa agtcttacat gcaatagttg
     5161 ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt ttgcagtact gcgcaaactc
     5221 gtgtttttgt atgtggcccg aagaaagttc atattttaat cgtggtgtgg tagaaggtat
     5281 cttaacaaaa aaccacaatg cgagattaag cggatatatt tttgtcgatt tttcagtaag
     5341 ttttttccgt ctattccttg aaaaagactg gattgactat cttgcaagta ctgacatggg
     5401 gattgtttta gtcagcgacc gcaatatgca atcgttagct aattactggc gaaaacacaa
     5461 ctcagcaata tctgctgtca tatataatga tgatggtctt gatgttgcca atgaaaagat
     5521 cagacaacta tttattggtc gttatttatc atttaccaga ggtaacacat taactcagat
     5581 ggaattcacc ataatggggt atatggtttc tggttataat ccatatcaga ttgctgaagt
     5641 tctggatatg gatatccgta gcatctacgc gtacaagcaa cgaatcgaaa agagaatggg
     5701 tggtaaaata aacgaattat ttattcgttc acattcggtc caacattgat actcaacgac
     5761 cagccagaat catactctgt ttatacgggc atttattgaa atgtccgtat tttataaata
     5821 ggccatcccc tcaagagtaa acaccatagt atttaaaacc atcacacgaa aaattcagaa
     5881 gcattacgaa atatgaattt acaaacaata gtggcataaa tgttaaccat gttaatttac
     5941 gtaaagtttt acgttgcaac attaaagcct catttcaatc atcatgataa atataaaatt
     6001 aatatatatt tatgccgtaa ataccaatat acttagcaaa ctatgtgatc t
//
LOCUS       DAZFZC010000183         6047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFZC010000183 DAZFZC010000000
VERSION     DAZFZC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.185"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..582
                     /gene="nleB"
                     /locus_tag="ACHKXP_004858"
     CDS             <1..582
                     /gene="nleB"
                     /locus_tag="ACHKXP_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO7649677.1"
                     /translation="ENIHKQESYPASERDNYLLGLLREELKNIPEGKDSLIESYAEKR
                     EHTWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDG
                     IAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFN
                     YSSLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            631..1305
                     /gene="nleE"
                     /locus_tag="ACHKXP_004859"
     CDS             631..1305
                     /gene="nleE"
                     /locus_tag="ACHKXP_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO7649678.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSKIE"
     gene            1683..2660
                     /locus_tag="ACHKXP_004860"
                     /pseudo
     CDS             1683..2660
                     /locus_tag="ACHKXP_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3179..>6047
                     /gene="efa1"
                     /locus_tag="ACHKXP_004861"
     CDS             3179..>6047
                     /gene="efa1"
                     /locus_tag="ACHKXP_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO7649679.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHKQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNSLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEIIEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAITDIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFNSFFLENMIKLQDNFFNYCIV
                     KGVTEINDELRINYLKNVIKLSDDDIGNYQKTINDNKDRVKKLILDLQKQFGENRISI
                     KDVNSLTSLSKSENNHNYQTEMLLRWNYPAASDLLRMYILKEHGGIYTDTDMMPAYSK
                     QVIFKIMMQTNGDNRFLEDLKLRRAISDGVLRYVNNQNIDEVNYNEISDADKNIIKKI
                     LTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDA
                     VIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFSKFR
                     QDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTTGT
                     GKYDWLTPESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSTLKTENLPELFLTKIDT
                     DTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTIDFKNLYEIDQMLHMIMLEMNDDI
                     AKRSLFSLQVQIAEKIRRMTIPVDNIINIYPDLHKKNDNDLS"
BASE COUNT         2120 a         1087 c         1192 g         1648 t
ORIGIN      
        1 gaaaatatcc ataaacaaga atcataccct gccagtgaga gggataatta tctgttaggc
       61 ttattaagag aagagttaaa aaatatccca gaaggtaagg actctttgat tgagtcatat
      121 gcagaaaaaa gagaacatac ttggtttgat tttttcagga atttggccat gttgaaggct
      181 ggaactttgt ttacagagac gggaaaaact ggatgccata acatatcgcc ctgtagcgga
      241 tgtatatatc ttgatgccga catgattatt accgataaat taggagtcct gtatgctcct
      301 gatggtatcg ctgtgcatgt agattgtaat gatgagataa aaagtcttga aaatggtgcg
      361 atagttgtca atcgtagtaa tcatccagca ttacttgcag gcctcgatat tatgaagagt
      421 aaagttgacg ctcatccata ttatgatggt ctaggaaagg gtatcaagcg gcattttaac
      481 tattcatcgt tacacgatta taatgctttt tgtgatttta ttgaatttaa gcatgaaaat
      541 attataccga ataccagtat gtataccagc agttcatggt aagtcttccg gttgtggttg
      601 aaaaatatcg ttctttaggt atagattcag atgattaatc ctgttactaa tactcagggc
      661 gtgtccccta taaatactaa atatgctgaa catgtggtga aaaatattta cccggaaatt
      721 aaacatgatt actttaatga atcacccaat atatatgata agaagtatat atccggtata
      781 accagaggag tagctgaact aaaacaggaa gaatttgtta acgagaaagc cagacggttt
      841 tcttatatga agactatgta ttctgtatgt ccagaagcgt ttgaacctat ttccagaaat
      901 gaagccagta caccggaagg aagctggcta acagttatat ccggaaaacg cccaatgggg
      961 cagttttctg tagatagttt atacaatcct gatttacatg cattatgtga gcttccggac
     1021 atttgttgta agatcttccc taaagaaaat aatgattttt tatacatagt tgttgtgtac
     1081 agaaatgaca gccctctagg agaacaacgg gcaaatagat ttatagaatt atataatata
     1141 aaaagagata tcatgcagga attaaattat gagttaccag agttaaaggc agtaaaatct
     1201 gaaatgatta tcgcacgtga aatgggagaa atctttagct acatgcctgg ggaaatagac
     1261 agttatatga aatacataaa taataaactt tctaaaattg agtagagaag ctcagccact
     1321 tgtgtagggg ggctaaaatt aattctattt cattatatga gaacaccaat aagaataata
     1381 atatttgatc acaactgaag atgcttttta caatgaagaa ttattgttaa aaaacgcttt
     1441 gttgaatcaa ccgaactgtt gctgagttgc aggatcacat cacgtatttt cctgaaaacg
     1501 caggctgctt agtagcaaag cagaagttca aaatcaccaa ctaaatagct gcgccgaata
     1561 gtagatcaat gaaagggaac tcagcccgga ttgtgcgatc tgatcaatcg ccaaaaatcc
     1621 ccccctaacc ggactgagcg atgccgatca tagcaccaat tccccgcgac gaatgacgcc
     1681 tgatgcaaaa agcaatctat aaaactcacg ataaaaatta tgcccgcagg ctgaccgcca
     1741 tgctgatgct gcaccggagc gctcgcgtca gtgacgttgc cagaacgctc tgctgcgccc
     1801 gttcctccgt caggcgctgg attaactggt tcacgctgtc gagtgttgaa ggtctgaaat
     1861 cattacccgc aggacgttcc cgccgctggc cgtttgagca tatctgcaca ttgtttcgtg
     1921 aactgataaa acactctccc ggatattttg gctatcagcg ttcacgctgg agtacagaac
     1981 tgctggcaat aaaaatcaac gagataaccg agtgccagtt acatgccgga accgttcgcc
     2041 actggttgcc attagtcggg cttgtgtggc gcagggctgc gccaaccctg cgtacccgta
     2101 acccgcataa agcagcaatt cacaaagcac tggacgaatg tagtgcggag catccggtct
     2161 tttatgaaga tgaagtgtat atctatctta atcccaaaac cggtgcggac tggcaacttc
     2221 gcagacagca aaaacatgtg gtaacaccgg ggcagaatga aaaatactat ctggctggtg
     2281 ctttacatag tggaacgggc aaagtcagct atgtgggtgg caacagcaaa agctcagcct
     2341 tatttatcag tctgttgaaa catctgaaat tgacataccg acgggataaa cctatcacgc
     2401 tcatagtgga caactacatc atccataaaa gtcgggaaac acagcgctgg ctgaaagaaa
     2461 atccgaagtt cagagtgatt tatcagccag tttactcgcc gtgggtgaat catgttgagc
     2521 ggttatagca ggcgcttcac gatacgataa cgcgcaatca tcagtgccgt tcaatgtggt
     2581 aactgcttaa aaaggttcat tattttatgg aaaccgtgag cccattcccc ggtggtaaat
     2641 atgggctgtt aaaagtgtag cggtattcgg cgcagctatt taggatggtc aggtcgcaga
     2701 agttctgtgc cactacagtg cgtgtactaa acaaaatgac gagggcacga atgtcataat
     2761 acgtgcgtat tacctgcagt agctaccaac tgagggggct tatcccaaac ctgatttatt
     2821 caacaaagct ggacttatcc ttactctgaa ttattttcca gacatatgca tcatgtactg
     2881 gctcgcttgg ttcaggaaat acaggagcaa atctacagct attagattta ttcctatttg
     2941 tatgcaaagt cgcattgcat aatgcggagc tatgttacag atacgcctga cgtgcgtagt
     3001 aaaattataa agcaacatat atatacaagg agaaaccgag aaaaataaat gagtcgtatg
     3061 ttgggtagca tcgagataaa tatttttttt atcacattag caccttggtt aagggtgtta
     3121 ataataatat tctctcactt attaaaacat aaataatttt ttaagcggaa atgtaattat
     3181 gagactgcca gagaaagttc tttttcctcc tgtcactagt ggcctgtcag ggcaggaaaa
     3241 acaaaaaaaa ccgaagagca ttaccggatt tcaggaaaat tatcaacgca atatcaggcc
     3301 aatcaaaaca gcatcagaag cccgactacg cttctttgat aaaatggttt cgaaagaaaa
     3361 ctctctggaa gatgttgttt ctttaggtga aatgattcag aaggaaattt atgggcataa
     3421 acaaagaaca ttttcaccag ttcatcatac aggtaactgg aaatcatcat tgttacacaa
     3481 cgcgctcctt ggtctggcaa atgtttataa tggcttacgg gaaacagaat accctaacac
     3541 tttcaacaga gatggtataa aaagtactaa ctcttttaga gataactcat tgacaaaaac
     3601 aagaactccc agagataatt ttgaggaagg aataaaacat cctgaacatg caacaatacc
     3661 atatgacaac gacaatgaaa gtaataaatt gctaaaagca ggaaagatag ctggtaacaa
     3721 taacgagctg ttgatggaaa taaaaaagga atcccaaagc gaccatcaaa tccccctgtc
     3781 agataagttc ctgaaaagga aaaaacgatc tcctgtagct gaagataaag ttcaaaactc
     3841 gttaacacca gaaaattttg ttcagaaaat ttcacttagt gatgagctta aaacaaaata
     3901 tgcaaatgaa attatagaga taaaaagaat aatgggagaa tacaatcttt taccggataa
     3961 aaacagtcgt aatggtctaa aacttctaca gaagcaagct gatttactaa aaataatcat
     4021 ggaggatacc tctgttacag aaaatacctt taaaaacata gagatggcta taacagatat
     4081 aaaaagggag tactattcgc atacagttga tattgagaaa aatattcatg ccatatgggt
     4141 tgcgggttcc ccacctgaaa gcatctcgga ctatattaag acttttctca aaacctacaa
     4201 agagtttact tactatcttt gggttgatga aaaagcattt ggagccgcga aatttaccag
     4261 tgtcttaaaa caaattgcat ttgatttagc atgcagaact atacagcaga atactccaca
     4321 aaaaaatatt gattttatta atctatataa tgaaataagg aagaaataca acaacaaccc
     4381 atcgggacaa caagagtact taaacaaact cagagagctt tatgctactt atcaaaaaat
     4441 atccacccct ctgaaacaca tgtttaattc atttttttta gaaaacatga ttaaacttca
     4501 ggataatttc ttcaactatt gcattgtcaa aggtgttaca gagattaatg atgagttacg
     4561 aataaactac cttaaaaatg taataaaact gtcagacgat gacattggta attatcagaa
     4621 aacaattaac gacaataaag atagagtaaa aaaactaatt cttgatttac agaaacaatt
     4681 tggtgaaaac cgcatttcaa ttaaagatgt taactcttta acctctcttt caaaatcaga
     4741 aaataatcac aattatcaaa ctgaaatgtt gcttcgatgg aactatcctg ccgcctcaga
     4801 cctgctcagg atgtatatcc ttaaagagca tggtggtatt tatacagaca cagatatgat
     4861 gcctgcatac tctaaacaag taatttttaa aattatgatg cagacaaacg gagataatcg
     4921 tttcttggaa gatttgaaac tacgtcgtgc aatatctgat ggtgtattaa gatatgttaa
     4981 taaccaaaat attgatgaag ttaactataa tgaaatcagt gatgcagata aaaacattat
     5041 caagaagata ttaacagaaa tatctaaaat gccagaagat agtattttta ctaagatcaa
     5101 tacaaggatt cctcgagaca caatgcccat ccttcgtcgt tatcacctat ggcctgatgg
     5161 atggaatatt cgtgggctca atggattcat gctatcacat aaaggtagtg aagtgattga
     5221 tgctgttatc gcaggccaga atcaggctta cagggaacta agaagaataa gagataatat
     5281 tcatagtgaa atatacttca aacaaactga tgaattgtcc tcacttccag atacagacaa
     5341 aattggaggg attctggtaa aaaaatacct ttcaggaagt cttttttcaa aattcagaca
     5401 agatactatt atcccggagg cattgagcac cctccagata tcaggtcctg acttgattca
     5461 aagaaagatg ttgcaatttt tcaggagtag aggggtgtta ggtgaagagt tcattaatga
     5521 aagaaaactg agtgataaag cttatattgg tgtctacaaa acaactggca cagggaaata
     5581 tgactggtta acccctgaat cgatcggcgt taatgatgtc acgcccgcag atgaaagtac
     5641 ctggtgtata ggaaaaggcc ggtgtgttga tgacttcctg ttcaaagatg tttcaacact
     5701 aaaaacagaa aatcttccag aattattctt aacaaaaata gatactgata cgtttttctc
     5761 tcagtggtca accaaaacca agaaagatct gcaaaaaaaa atacaagacc ttactgtacg
     5821 ttataatgag ctaattgact cgtcgactat cgactttaaa aatctatatg aaatagatca
     5881 aatgctccat atgattatgc tagagatgaa tgatgatata gccaaaaggt cattgttttc
     5941 attgcaagtt caaatagccg aaaaaattcg gaggatgacc attcctgtag acaatataat
     6001 taacatctat cctgatctac ataaaaaaaa tgacaatgat ctgagta
//
LOCUS       DAZFZC010000184         5980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFZC010000184 DAZFZC010000000
VERSION     DAZFZC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.186"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..236
                     /locus_tag="ACHKXP_004862"
     CDS             <1..236
                     /locus_tag="ACHKXP_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
                     /protein_id="HFO7649680.1"
                     /translation="EQLYPAQEHLLLQCSYDDKYDFNASTHRGIIGESLTRLGAMFSG
                     KEYQILLPGEIHIKESGKREYKGRDSGLFMQPL"
     gene            complement(244..597)
                     /gene="cmi"
                     /locus_tag="ACHKXP_004863"
     CDS             complement(244..597)
                     /gene="cmi"
                     /locus_tag="ACHKXP_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO7649681.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
     gene            complement(770..1552)
                     /locus_tag="ACHKXP_004864"
     CDS             complement(770..1552)
                     /locus_tag="ACHKXP_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7649682.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(1554..1967)
                     /locus_tag="ACHKXP_004865"
     CDS             complement(1554..1967)
                     /locus_tag="ACHKXP_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649683.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2526..2756
                     /gene="vapB"
                     /locus_tag="ACHKXP_004866"
     CDS             2526..2756
                     /gene="vapB"
                     /locus_tag="ACHKXP_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO7649684.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2753..3169
                     /locus_tag="ACHKXP_004867"
     CDS             2753..3169
                     /locus_tag="ACHKXP_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO7649685.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(3331..4023)
                     /locus_tag="ACHKXP_004868"
                     /pseudo
     CDS             complement(3331..4023)
                     /locus_tag="ACHKXP_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(4638..5498)
                     /locus_tag="ACHKXP_004869"
     CDS             complement(4638..5498)
                     /locus_tag="ACHKXP_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7649686.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
     gene            5625..>5980
                     /locus_tag="ACHKXP_004870"
     CDS             5625..>5980
                     /locus_tag="ACHKXP_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7649687.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
BASE COUNT         1551 a         1525 c         1399 g         1505 t
ORIGIN      
        1 atgagcaatt atatccagca caggaacatc ttcttttaca atgttcatat gatgataaat
       61 acgattttaa cgccagcact caccgtggca tcatcggaga gtcgctcaca aggctcgggg
      121 cgatgttttc tggtaaagag taccagatac tgcttcctgg tgaaattcac attaaagaaa
      181 gtggtaagcg agaatataag ggccgggatt ccggcctttt tatgcagcct ttataacagt
      241 gtattagtca ttcgcatatt caacatatcc ctcctcacaa tggtatatgc cataatacat
      301 tctcttaccc tccgggcctt cagctttaaa tgggacaaga agtgtttttt catcttcaac
      361 atctgttaaa cttctctcaa tcttttccca tgtaattttc gggatttctg taccaagaag
      421 ttgaacatcg ttgtcccagc gtttcattct ttgcaggtaa tcatttttta caaacgttct
      481 ggcctgttca tcgctgacct gatagcatgc cggaccttta tcttcagacc agaaaaaaac
      541 agcagcaaga gcaataatcg ttaaaataaa aataaatttc atgctaatta ctttcataaa
      601 atctccacaa cttctgtcgt tcattcgata taaaaaaaca ttacgaatat atccgtacaa
      661 tgtgagcata ctgtgaagtg gctttaacaa ttatgaaata atacaagaga tcacatattg
      721 cagtggtctc ttttttatag aagaaatcac aattcataat tgtgacttat tatgcatgtt
      781 tgtttttcag cattgtgacc gcatcggact caggcatcga aaactgcacc cggtgccggg
      841 cagcgacatc cagcgcaaac accttcgtgt agacctccgt tgagctgatg gacttatgcc
      901 ccatcagact ctgcaggacc ttcagcggta taccggcata cagcatatgc atcgcatagg
      961 aatggcggaa cgtatgtggc gtgaccggga cagagaacgt cacaccatca gcggcagcgg
     1021 cggcaaccgc ctccccaatc caggtcctga ccgttctgtc cgtcacttcc cagatccgcg
     1081 ctttctctgt cctgcctgtt cgtttattac gccgttccat ggggattttc aatgtggcca
     1141 ccatcgtctg cagctggctg acgtaccagg agtcggagag cggaaccagc cggtgagtct
     1201 gctgcccggc gggcgttctt cctgccgtcc tggcggcttt ttcggtccgt tgtttcaggg
     1261 tcgcaagctg cacaaacgga tacggaggcg tgagcgaaaa atccccccgc gtcagcgcca
     1321 gtgcttcatt aatgcgcgca ccggtgttcc acagtgtggc cagcagcatc ttgcggcgca
     1381 gatccgggac gtaatggagc agggcgctca cttcaggggc cagcaggtat ttgggcagtt
     1441 catcatgaac catcgacatc tggcggagtg ccagcgctgc cggataatcg atggcaacag
     1501 gaagctgagc ggcatcccgg gcatggtgga tcggtgccgg caggtgctgc atatcagtgt
     1561 tcactccacg gggaaacgtc gggcaggttt tccacaaccc ggtactttcc cggataacgg
     1621 agctggttac cgccacgcgt ccagacggtg acaacaaaac cccctccctg gttgttttcg
     1681 gggagcaggc gatcgcactg ctcactcagc atgtggatca cgaacggcgc atcgctgccg
     1741 tcttcaaata tcagttccag cgcatcgcgg ccatgcagcg ttcccttact gatgatgacg
     1801 tactcagccc cccgcatctc cctgagtaaa tgttttgccg catccgggac aagcagcctg
     1861 gctgcgccgg cattccagct gaggtagaca tatccggcct gcgcctgtac agactgccag
     1921 taatccgttc tggcgacagc ctgaccttca ttctcaataa aaaacatggg tgactccttt
     1981 tttcttttcc gggcagggac ggtcatgcga taacgtcatc catcatcact gctcttaccg
     2041 tcacgccatg tgataacatc atgccgttat catcatcacc atgataacaa gcatccatat
     2101 aaccacgctg ttaaattacc ggaggaccat ttccgacaga tccgattttt ctgttattga
     2161 cgggttttcg gaagtgtatc acgctgagga aataatcata tcgctgtttt ccgcccctgc
     2221 gcgacgcatc acaggtcagg gagatgacac aacatatagt gtcatcagca gatgatccgg
     2281 caccacatgt tgtgatgacg ggaggtggcg ttgtgtgggg ggagaggaag atcctgaaca
     2341 accttttttc ggatctccga taaaaaagaa agaaatagag atatgctaaa catatgcctg
     2401 gcatctacat atgctaccgc tatacatatg ccacacatct gcctatacta aacatatgca
     2461 cacacccggt gctttgaata ttccggatat gtttcacata tccatatgct cgggagagaa
     2521 agaacatgag aaccgtatcg atatttaaaa atggcaacaa ccgcgccatc cgtctgcctc
     2581 gtgatctgga ttttgagggg gtgagcgagc tcgagatcgt ccgggaaggg gacagcatca
     2641 tcctgcgccc cgtccggcca acctggggat cattcctaga gtacgaaaaa gccgatccag
     2701 attttatggc ggagcgcgag gatgttgtca gcgacgaagg gcgatttaac ctgtgaacaa
     2761 gacttatatg cttgatacct gtatctgctc gttcatcatg cgtgaacagc cagaagccgt
     2821 cctgaagcgt ctggagcagg cggtgctacg cggccaccgc atagtggtct cggccatcac
     2881 ctactctgaa atgcgcttcg gggctaccgg cccaaaggcc tcaccacgtc acgtccaact
     2941 ggtcgatgaa ttctgcgccc gcctcgatgc catcctgccc tgggatcgcg ccgcagtaga
     3001 cgccaccacg aagattaaag tggcactgcg gctggccggg acgccgatcg gcccgaacga
     3061 cacggcgatt gccgggcacg ccatcgccgc cggggcgata ctggtaacga ataatacaag
     3121 agaatttgag cgggtgcctg acctggtgct ggaagactgg gtgaagtaag acactctttt
     3181 caaagtcagg agataagggc accaagaaac agcccgtata cgggctgttt taacagatat
     3241 ctacagtaag cggtcctaaa ctgtagggta tcttcgaaga tcagactatc agggagctaa
     3301 agcctatctt ccggcggcaa aaacagtggc tcaagcgata cgggcaaccg agctctggtt
     3361 tgctgccggt ggcagggcga tgagcttgtc acattccata atcacgtact ccgggatgag
     3421 atcgaatctg gacggatcta gttgacagat gaactcattt tcaatgtggt acgtttcgtt
     3481 aacgaatttt cgtattaccg gatggtcctg atccagttcc agtaaacgga atacctgcag
     3541 tttttcatag ccgttacgac agcgacggta caggtttttt atttcatctg ggctgctgac
     3601 gagagcctga agtcgcgggt aatcaaaact gtccacatac tcccggatat cccgcaaggc
     3661 ctgctgaatt ttctcaggag ccattttggg gtagcccgag cccgcagcgg caggctcgcg
     3721 ataatccagc ggggcgacgc gtcgatggaa tagattggat agaagctgat aagcatcacc
     3781 acgttcgtca acgatttcaa agtagcgacg aagatagatg agcttaatga tctcctcaca
     3841 gtctgctgac gcagtgatgg atttgcagat ctgcatgaat gtcatgatat cgccgtcgtt
     3901 aacaggtaat tcttctatga ctccagcaga caggcgcagg caggaggcgg tcacctgatt
     3961 actgaacaac cttctgactg acttcagcgt gtcaatcaca ggctccacgt catgcgtcag
     4021 catagtgctg atgctgacgg atgttacaaa aaatgcagcg tatggcgttg agatagaaag
     4081 tctggtgctg gagataaata cacctacatc atgataaaaa agggccagcg actgacctga
     4141 agaagacact ggctaaaagg ctttgcatgt ttgtagatac ttatttttca cagacagcaa
     4201 tgatgcctgt caatatatta ttaatatgct gattgtttca gttacagatg ctttattaag
     4261 ggaaaatgtc acgatcggtg caaaagtggt ttgaaccgcc ccggttttcc tgaaggctgg
     4321 gtcatatccc tccggcagaa gcagaaaaag cttattatgc ttccatcaga aacgatgatc
     4381 tggcagcctg agttcacaga taaaacactc tccaggaaaa ccggggcggt tcagacattt
     4441 cagagatggt tatttttttc tgtgaacacc agtgtccgtg tcccccatac ctgcagcttg
     4501 gtaaaaagtg gggggctggc aatccagtct gcctcccgga aatgaacgat gcctctttat
     4561 tataaagttt agtgaccgcc ttgacggtca cttttaagaa aaagtattta cacagaatgg
     4621 taaacacagg ctcactatta aatatatttt ttaaaccatt gtgtttgcac agctgctacc
     4681 cgctcaaaaa atgtgccctc ataaatatca taatggcatg cgtcagcttc ctcatataat
     4741 tctttagtac cagaagcaac agcatcatat aatgctcttc cctgttccgg tggattaaca
     4801 ctatcctggc cagcaataac aaccaatact ggacactgga ctctggctgc gctctcggcc
     4861 ggtttatatt gcaacgtttc catgacagta aggaaaggta tttttatatc catttcggga
     4921 tgctgagctt tgactttctc gaagaatacc ttcgactcat catcatttag tacccgtgta
     4981 acccccacaa acatctcctt ccctgtgttt ttctttttct ctgccatttt attcagtgtg
     5041 gacagaaaag aagctctctc caattcattc atctctccgg taatcagagc atcaccatca
     5101 gcaaaagcca gctggctgac aatacatttc actctttgat cttgcgcagc tgcactgaac
     5161 acatgacaac cacccagcga tgttccccat aacccgatac gctgattatc aatacatgcc
     5221 tgcttttctg cccaattgat aactgaaata atatcttcga tctgcattgc tggaaccaga
     5281 cggccccgtt ctccttcact ctcaccgaat ccacgataat caaaagtaat cgttgcgaac
     5341 cctgcttctg tgaaagcatt cgcaaagcaa gggagcagaa cattccggat accgcaaaat
     5401 ccatggcata agataatcaa aggatgtttc atatttcctt caggaacacg aaatgtcaga
     5461 gcaatacctt cagaaagttt gtgatccgta attttcataa atatcctgtg ttatgttgtt
     5521 tattaatcat cagaatcagc gccagcatca taccagtcac aataccagaa tgcccagcgc
     5581 aatcagatcc ataaacagtg cgtgtgctac gtgaaaaata actcatgaac aatgtcattc
     5641 ccctgcagaa ttcaccagaa cgcgtctccc tgttacccat tgcgccgggg gtggattttg
     5701 caacagcgct ctccctgaga agaatggcca cttccacagg ggccacaccg gcctacctgc
     5761 tggccccgga agtgagtgcc cttcttttct atatgccgga tcagcgtcac catatgctgt
     5821 tcgccaccct ctggaatacc ggaatgcgta ttggcgaagc ccggatgctg acaccggaat
     5881 catttgatct ggatggtgta agaccgtttg tgcggatcct gtccgaaaaa gtgcgcgcgc
     5941 gacgcggacg cccgccaaaa gatgaagtcc gtctggttcc
//
LOCUS       DAZFZC010000185         5963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFZC010000185 DAZFZC010000000
VERSION     DAZFZC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.187"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            124..1221
                     /locus_tag="ACHKXP_004871"
     CDS             124..1221
                     /locus_tag="ACHKXP_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7649688.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1411..1794)
                     /gene="quuD"
                     /locus_tag="ACHKXP_004872"
     CDS             complement(1411..1794)
                     /gene="quuD"
                     /locus_tag="ACHKXP_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO7649689.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1880..2020)
                     /locus_tag="ACHKXP_004873"
     CDS             complement(1880..2020)
                     /locus_tag="ACHKXP_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO7649690.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2017..2379)
                     /gene="rusA"
                     /locus_tag="ACHKXP_004874"
     CDS             complement(2017..2379)
                     /gene="rusA"
                     /locus_tag="ACHKXP_004874"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO7649691.1"
                     /translation="MNTYHITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2376..2666)
                     /locus_tag="ACHKXP_004875"
     CDS             complement(2376..2666)
                     /locus_tag="ACHKXP_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO7649692.1"
                     /translation="MADLRKAARGRECQVRITGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2659..2829)
                     /locus_tag="ACHKXP_004876"
     CDS             complement(2659..2829)
                     /locus_tag="ACHKXP_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7649693.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2829..3284)
                     /gene="ybcN"
                     /locus_tag="ACHKXP_004877"
     CDS             complement(2829..3284)
                     /gene="ybcN"
                     /locus_tag="ACHKXP_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO7649694.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQIQPYLEDGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3281..3382)
                     /locus_tag="ACHKXP_004878"
     CDS             complement(3281..3382)
                     /locus_tag="ACHKXP_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649695.1"
                     /translation="MRTYNPNSLLPSQMQKCTCDFLHPASDLCGGEA"
     gene            complement(3475..3927)
                     /locus_tag="ACHKXP_004879"
     CDS             complement(3475..3927)
                     /locus_tag="ACHKXP_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649696.1"
                     /translation="MKRLFVIAPLLVLVGCAQNISPNSYSVGSVGMVNRTIAGTVISA
                     RGVDISGTSALGGTAGAAVGATAGSALGGGVRSNIVGAVGGAVIGGIAGAAIESSATK
                     QTGMEYVVETENGNLMTIVQGKDPLFTQGSKVLVLYGNPSRIITDPRH"
     gene            complement(3924..4484)
                     /locus_tag="ACHKXP_004880"
     CDS             complement(3924..4484)
                     /locus_tag="ACHKXP_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine acyltransferase"
                     /protein_id="HFO7649697.1"
                     /translation="MKKIILLAMIIGSLTGCASVPPLNFSTPNVGVSQKKIDAEIKSL
                     TVSLARPDEQKGDITAGMEAITPIWRESLQEALDRMTIFRDSSPNTVSLNVKVLALDV
                     PAFGVSMTTKAIARYEIINRANGDIIYTQDIESTGTVPASYAFYGIVRARESVNRAVQ
                     NNITQFLQALESVDLSRPMFPVRVAK"
     gene            complement(4970..5263)
                     /locus_tag="ACHKXP_004881"
     CDS             complement(4970..5263)
                     /locus_tag="ACHKXP_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7649698.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARTG
                     ILVIDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAFYRGNFQ"
     gene            complement(5260..5961)
                     /locus_tag="ACHKXP_004882"
     CDS             complement(5260..5961)
                     /locus_tag="ACHKXP_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7649699.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDSELRRKAADELAHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALTK
                     IAEIKAKFGLKGASV"
BASE COUNT         1587 a         1461 c         1255 g         1660 t
ORIGIN      
        1 atctacttca caaatcaaac gagaaaccaa aacttacatc ttgaaataat cacattgatt
       61 agatgaatat ttatcgcgca gtgacatcat tttttaataa tagttcaaaa aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcagtact gatggcgatg
      181 tctgctcagg cagctgaaat ttataataaa gacagtaaca agctggatct gtacgggaaa
      241 gttaatgcta agcactactt ctcctctaat gatgcagatg atggtgatac tacttatgcc
      301 cgtcttggct tcaaaggtga aacccaaatc aacgatcagc tgactggttt cggtcagtgg
      361 gaatatgaat tcaaaggcaa ccgcgctgaa tctcaaggtt cctccaaaga caaaacccgt
      421 cttgcatttg caggcctgaa attcggtgac tacggctcaa tcgattacgg ccgtaactac
      481 ggtgtagcat acgacatcgg tgcgtggact gacgttctgc cagaattcgg tggcgatacc
      541 tggacccaaa cagatgtgtt catgactggt cgtaccactg gtgttgcaac ctatcgtaac
      601 aacgacttct ttggtctggt tgatggtctg aactttgctg ctcagtacca aggcaaaaac
      661 gatcgtagcg atttcgataa ctacactgaa ggtaacggtg atggcttcgg tttctctgct
      721 acctatgaat acgaaggatt cggtatcggt gcaacttatg cgaaatctga tcgtaccgac
      781 actcaagtta atgcagggaa agttcttcct gaagtatttg cttccggtaa aaatgcagaa
      841 gtttgggccg caggtctgaa atatgacgct aacaacattt acctggccac tacctattct
      901 gaaacccaga atatgactgt atttgctgat cacttcgttg ctaataaagc tcaaaacttc
      961 gaagctgttg cacaatatca gttcgatttc ggtctgcgtc cgtccgttgc ttacctgcaa
     1021 tctaaaggta aggatcttgg agtatggggc gatcaggact tagtcaaata tgttgatgta
     1081 ggtgcaacct attacttcaa caaaaatatg tctactttcg ttgattacaa aatcaacctg
     1141 cttgacaaaa atgacttcac taaagcactc ggtgtaagca ctgatgacat cgttgctgta
     1201 ggtctggttt accagttcta atctgattac gaaaaagata tgttgcggga ggctttgcct
     1261 ccccaacata taagtggctc cctcaagcca cttcctttag gagcacaacc ttgcttctaa
     1321 ctatataaac cttctgttat atattaccct ttatttttgg gggcgttgca acgccccatt
     1381 tttaataact tttagtaaac aactggcata ttaattagag ttattaacaa cgatatccat
     1441 ctctaaccgg atatctaatg ccattaacat cccttcaatt ataccctcag ccttctgtaa
     1501 ccttttcccg atataaccat cagagcagca atgcttacct gccagtgaca tgaatgtcat
     1561 accgactaca taataatcta ctaataaatc gtgcaaatcg ctgttgttct ttttcagacg
     1621 ggccatgcac ccgcaaatga tcatcgcgtc atcgtcacaa cattgcgggc gagattttac
     1681 ttttgaagta attaatccct taaaaccggc ggcaatggac gaccaggtca catcttcatg
     1741 attattagcc gcccacgctc cccaacgctc aagaaccatc tgaatatcac gcatcaactt
     1801 actccacaaa aatcagacca gaacgccaat tacaagcaaa aatcaacaaa atagtattag
     1861 ttgattgtta tctctgactt catactcctg ctcctgtcag ggttttggcg taattcttca
     1921 gtatccggta atcggtcaaa acagaaccag gaaaacgata taagcgcagg cgcacccagc
     1981 ggcggcgaag acgctctgcc atataagact caaacatcat tcattcccca tttcggtgat
     2041 ggtcagttcc agcctcccac ctttggtaac aggcatcttc acaacgcggt aatcaacgac
     2101 ctgagcatca tccagccaga aacctgcttt ggtgagtgcg tcaaaagcgg ccttttgcag
     2161 attatccagg tcacggcgac ggcgatccgg catgtggcac tcaatacgga ttttcactgg
     2221 catagccagg ccgatatcca gcattgcgtt tttaatgatt cgggtgacgt tatcgcggta
     2281 tgcctgccct tctgcgctga tgtgtgtgcg cccgcgatta tggcggtaat agcggttatt
     2341 gctcggaggc cagggtagtg tgatgtggta agtattcacg ccttgattac cccctctttc
     2401 agccagataa cctgcgttct cgccatacct tccagcgcgc attcttttgc gtactcagca
     2461 tcgacaaaat gcgtgcggcg gtcgatttcg tcgtgacagg cagaacaggc aatggtggca
     2521 ataaggtctg gcggtttaat accggtgccg cacaatccag ccagccggat atgtgccagt
     2581 acagacgttt cagggttgcc attacatacg ccagtgattc ttacctggca ttcccgacca
     2641 cgcgctgctt ttctcaaatc agccatgatt cctccttgct gccagtcgca accatttttt
     2701 atcaaccagg ctagcggtat atccgagcag tgttggtatt tcggatggct tcagctcagg
     2761 cttacgctta cgacgatttg gtactctgta gatgtgtccg ttcatgacac gaataagcgg
     2821 tgtagccatt acgcctcctg cttgtcgcgc agcagctgga actcgcagct ctgcggaata
     2881 gtcaggtggc agccaatatt catcgcccag gcttcaacct tacacaggaa gacatacatc
     2941 tctccggtat caagatcgga agtatggcgt aacgactgga tagtagtgat ttcgccggtt
     3001 acgacatcaa ccaggtcctt ggtttcataa ccgaggtatg tgtgtttgag agcatctttt
     3061 acccatgctg cggtagcgaa cgatttcccc ctgctgatga ggtattcact gatttcgctg
     3121 taccacatgt ggctgagtgc attctgggaa agactgcgtt tctcacgcca cggtttaagc
     3181 accatgcgaa agcattttcc gtcctccaga taaggctgga tctgctggcc gatagcggtg
     3241 aagttaccgc gatgcagttt gatgccatct tgtggtaggt tcacgcttca cctccgcaga
     3301 ggtcagacgc tggatgcaaa aaatcgcagg tgcatttctg catctgtgaa gggagaagag
     3361 agtttggatt gtatgtgcgc ataaacgtcc ccgtttagcg cagaagtcac cggagttgtt
     3421 caagctccga tgactttatt attacgaatt gattttacaa aatcaaaagg tatgttagtg
     3481 acgcgggtct gttattatgc gagaagggtt tccgtataaa acaaggacct tacttccttg
     3541 agtaaataac ggatctttgc cttgaacaat ggtcattaaa ttcccattct cagtttcgac
     3601 aacatattcc atgcctgttt gttttgttgc tgaagattcg attgctgccc cggcaatacc
     3661 accaatgact gcaccaccaa cggcaccaac gatattagaa cgaactcccc caccaagcgc
     3721 agaaccagcg gttgccccca cggcagcccc agcagtcccg cctaacgcgg aagtcccact
     3781 gatatcaacc cccctagcac taataactgt accagcgata gttcgattaa ccatgcccac
     3841 agagccaaca gaataactat ttggcgatat attttgtgcg catccaacca acactaagag
     3901 tggagcaatt acgaataatc gcttcattta gctaccctaa caggaaacat tggacgagaa
     3961 agatcaacac tttctaatgc ttgcaagaac tgcgttatgt tgttttgcac cgcgcgatta
     4021 acagattcgc gtgctcgaac aataccgtag aatgcgtaac tggctggaac agtaccggta
     4081 gactcaatat cctgcgtata tataatatca ccattcgcac ggttgattat ttcatacctt
     4141 gcaattgctt tagttgtcat tgaaacacca aaagcaggaa cgtcaagagc caacacttta
     4201 acatttaagc taaccgtatt tggtgaacta tcacgaaaaa tagtcattcg gtcgagtgct
     4261 tcctgcaaag attcacgcca aattggagtt atagcctcca taccagcagt gatatcccct
     4321 ttctgctcat ctggacgagc aagtgatacc gttaatgact taatttcagc atctattttt
     4381 ttctggctaa ctcccacgtt aggtgttgaa aaattcaatg gtggcacact agcgcaacct
     4441 gttaaagaac caataatcat ggctaataat attatcttct tcataaattt accttattgt
     4501 tataaccaaa ggaattataa agtaaaaaag ttcactatca ctagccatta acgacatcaa
     4561 tttcagagaa acatggtact catttccaca aatttgacac aagtcatttt cacctacata
     4621 ttccatcata cttgatgcat atgttattga agcctctatc ctatccgttc ataatagcaa
     4681 tagttacccg ggtgatagta cctctatgat tactcgtctt tctgattgat tggattaaat
     4741 atgcgcgcca aaatttatca actttcgtta tggatattta tttcgtttct agcgatctat
     4801 gcctttatta tctataaagg ttcttatatt ggagtagcat tgcatcaaat tgcttggatc
     4861 atcattattg cctctggctt gattgctagg ctaactaaac caaagcaaaa accaatttcg
     4921 tccaataatt agacatgtat taaaaaaatg atatttttat gtacatagtc tattgaaaat
     4981 tgccgcgata aaatgccaac acccgcttca tcgcggcact ctggcgacac tccttgaaaa
     5041 tcagattcgt gctcaccttt ccttcccgtt cttccctggt agcgaaccgg taatacaccg
     5101 ttcgccagac cttaccatca ataactaaga ttcctgtccg cgccatttta gccgcagcct
     5161 gatttatgct ggttactgtt gcgcctgtta ccgcagcaac gtcctgcgca cagaagctct
     5221 tatgcgtccc caggtaatga ataattgctt cttttcccgt catacactgg ctcctttcag
     5281 tccgaactta gctttgattt ctgcaatctt cgtcagagcc tgtgcacgat ttagaggtct
     5341 accgcccatg acaggaagtt gttttactgg ttcaggtata gcctcaccac ggttaattct
     5401 cgcagtcata tgggcaagtt catcggcagc cttgcgccgt aattccgagt cagtcaacgc
     5461 attggcccgc atgttctgat acaggttggt aaccagccag tagtgcgcgt ttgatttcca
     5521 cggataagac tctgcatccg gatacagccc gcgcttccgg caatactcgt aaaccatatc
     5581 aaccagctcg ctggcgtttg gcagcccggc ggtaacggat gcttcttccc ggcaccaggc
     5641 gacaaactgc ccgggtgatg gcaggaatgg tcgattctgc cgacgggcta cgcgcattcc
     5701 agcgttaacc tgttccattg tggtgatccc gttttcccgg aaagccagaa cccactggcg
     5761 gcgaatttcg ttcagttcgt tctggtcacg gttagccagg ctcgcaggga aagttgccag
     5821 caactggctg aatacaccgt tgatgatctg cgctacctgc tgtacctgcg gcttttcgtc
     5881 gtactgttcc ggcatgttgt tggcgatccg acgcatctgc tcacggtcaa agttaatcat
     5941 ctgtgcggcg atgtttttca taa
//
LOCUS       DAZFZC010000186         5943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFZC010000186 DAZFZC010000000
VERSION     DAZFZC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.188"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..593)
                     /locus_tag="ACHKXP_004883"
     CDS             complement(<1..593)
                     /locus_tag="ACHKXP_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7649700.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGY"
     gene            complement(660..4058)
                     /locus_tag="ACHKXP_004884"
     CDS             complement(660..4058)
                     /locus_tag="ACHKXP_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7649701.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGSLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMVWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDAQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNARGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTEDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKMSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(4119..4691)
                     /locus_tag="ACHKXP_004885"
     CDS             complement(4119..4691)
                     /locus_tag="ACHKXP_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7649702.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(4688..5431)
                     /locus_tag="ACHKXP_004886"
     CDS             complement(4688..5431)
                     /locus_tag="ACHKXP_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7649703.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            complement(5437..>5943)
                     /locus_tag="ACHKXP_004887"
     CDS             complement(5437..>5943)
                     /locus_tag="ACHKXP_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7649704.1"
                     /translation="GFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGTVVRRKV
                     YARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGR
                     IMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGF
                     LSINKLSQ"
BASE COUNT         1243 a         1859 c         1516 g         1325 t
ORIGIN      
        1 ttataaccga cacccacgat gaaaccgtca gtgcgccagt cgccactgcc ggagccttca
       61 taagcaatat caatggccac ggattcggtc gggttaaact gcacgccagc tccccacgcc
      121 agagacgtgt tgctgtggcg accgtcatca cttccggtca gcacgtcgtg cgttttcccc
      181 ttgttgtcag ttacgcggag ataatccccg gagaaagtcg acacacggct gtaagccata
      241 cccgccatcg catacgcgct gaaccattca ttcacgcgca cagacggccc caccatcacg
      301 ctgaaccagc ggttacgaac ggaatcttca tgccagcggg tatcgctgta acgggtcagc
      361 tggcgattct tgtctcctgc atagctgaat gacgtcacca gccccagtgt gtccgtaaac
      421 tcataacggt atttcacgtt aatcccgttc agatcatcgc tgccgggaac gttcgtcgag
      481 gcatgaagat accccgcgct cagcgtggac tgatgttcag acgcccatgc aggcgcaccg
      541 gatacggcca gacaaatggc tgcggacaaa atggcggcat aaagtttacg cataattacc
      601 tctcgctttt ctgcaataaa aaaggcgtca tttctgacgc ccgttatggg ttataaaatt
      661 cagctgatac tgatacctgc tgtggatttc ttcatcacca caaccagcag atcgctgata
      721 ctggttgttg gtgtccagtt attcgctcct gatgaagata cggtgaatgt cagtgtcagt
      781 gtcccctgtc cggcaggcat atctataact gaagaaaata cgccctgagc atccgtcgtg
      841 gactgattaa aaatctcctg accattgcgg gtcactctta accggcaggt tgaataccag
      901 tatgactgtt ggttattact gttgaaattc tcatgcttac caccgcggaa taacactggc
      961 ggtatcatga cctgccggtc aaacttctga tcatcactga ttcttaccgt gatggtgccg
     1021 ctggcataac tgttcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcgccttca
     1081 atctgattgg ctgacatttt ccccttaatc tgacagttct cattaatcgt gacattgttg
     1141 agcgtcccgg agttcgcatt cacactgcca ctgatatccg catttttagc agtcagcttt
     1201 ccgtccggtg tcagggaaaa tgccggtgga tttccaccac tggtaatggt gggggccgtc
     1261 aggcgtttca ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc
     1321 gtttcattcc cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg
     1381 ctcagtttgc cttcctctgt atcctccatg ctgaggccaa gtcccgcgac ataatgctta
     1441 ccgtcttcgg tctgctcaat tttgacgccc cacatagcat tccatttatc gttagcgtcc
     1501 ttccactctt tcgaaaactc atccagtctg ctggcgttat cctccgtcag gtcgactttt
     1561 tccagcagct ctttaccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa
     1621 ccttccgcat catcgctcgc ccgaccgacg gcctccacga atgccgattt gccaacggtg
     1681 ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct
     1741 atccagtaca gccccgtgcc aaggtagcgg gctgtggttt caacctgcct gatatcggta
     1801 atccgcgttt ccgagaacca gaactcaaac tgtaccgtcg gatcataaac cgcaagatgc
     1861 ggcgtggcgg ttatctgaaa atagcccggc gtcagctcaa tccgcgacgg cgctgccggg
     1921 gcggcaatcc ggaacgatac cgacgccgga tcgccctgct gtccccgggc atttaccgcc
     1981 cggaccgtca gcgtgtaacg ccccagcgcc agttgcctga agcggtaagt ggtttccgtc
     2041 gtccgggccg tgctgaccag ccgctcactg ccgtcatcgg cggccacggt caggcgaagc
     2101 ataaagctca cgcccttcac caccttcggc gtatcccagc gcgccagcac ctggtattcc
     2161 ccgctgtctg cggtgacttc ggcggtcagg tgctgcaccg ctggcggcgt gacaccattc
     2221 accgtgccgc tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ttctttttcc
     2281 ggtacatgct gcacggcggt gatggcatac gtgccgtcgt cgttctcacg gatactcacg
     2341 cagcggaaca ggcgctggcg cagcgtcggc agcttcagcc cccacacgct gtattcagca
     2401 acgccgtcag gaacacggct cactttcacc ttcacgccgt cggtgacgga ctgaacctcc
     2461 acgctgaccg gattgccact tccgtcaacc aggcttatca gcgtggtacc ggaggatggc
     2521 agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc tgtttaccgc cagcacgcga
     2581 ccaccggtgc tgataccggc atagtcatca tcgcagattt caatgacatc gcccggtaca
     2641 tggcgaagcc cttcagcacc cacgctgaag tccacggtct gcgtttccag cagttccgtt
     2701 ttaatcagcc acagcccggc gcggtgcgcc tgcccccggc tggtacagcc aaaggcatcc
     2761 atcttcgtga cgttacgacc gtaacgggca atggcctgcg catcttcaac aagctctgtc
     2821 gccgtctccc agccgttatc cgggtcaatc cagttcacct caacggcatt atggcggtcc
     2881 ttcagggcgc tgaagctgta gcggaacggc gcgccatcat ccggcatcac cacattactg
     2941 cggttatagg tccacacctt atctgatggt cggtcctgca cgaacgtcag cgtctgcccg
     3001 ttccataccg gcatacagcg catcgccgag cagaaatcac tgagcacatc ccacgcctta
     3061 cgctgcgtgg tcaggtaggc attacaggtg atgcgcggct ccgtgccgcc aaaaccgtcc
     3121 ggcactgact ggtcgcagta ctggccgatg acatacagcg cccatttatc cacatccgcc
     3181 gcaccaagac gtttccccat gccgtagcgc ggatgggtca gcatatccca cagacaccag
     3241 accatgttgt tgctgtatgc cggtttaaac gttccgtccc agataccgct gtattgccgc
     3301 gtctgcgggt tatagttcga cggcacctgc agaatgcgcc cgcgcagatg ataattacgg
     3361 ctcacctgct ggctgccgaa ctgctccgaa tccacctgca cgccgaccag tgccgtgttc
     3421 gggtagccct gtttcacatc gatgatttcg gtgtatgacg accagagcgt tttgttctgc
     3481 agctggtctg tggtgctgtc cggcgtcatc ctgcgcatcc ggatattaaa cgggcgcggc
     3541 ggcaggctac ccaccaccac cgaggccaga tactgcgagg tggttttgcc cttaatggtg
     3601 atgtcttttt ccgtcaccca gccaccgtta cgttgtatct gaaccagcag acggacttcc
     3661 gacggattcc tgtcaccctt tgaggtggtt tccaccagtg cctgtacacc gaaggtaaag
     3721 cgcagacggt cgatgtttgc agacgtaatg gtgcgggtga tcggcgtgtc atatttcact
     3781 tccgtaccca gcaccgtctc ggagccggag gattcaaacc cctccggcgg tgtctgctcc
     3841 tgctcaccgg cacggaacac caccgtgaca ccggagatgt tggtattccc ctcagtgtcc
     3901 agcaccggcg tactgttcag cagcacgctt tttaatccat ccaccggacc ttcaaccggc
     3961 ccttcgctga tggcatcgat cacactcagc aactgcgtgg acttcaggtt gtccttcgct
     4021 tcgcgcgggg tatgcccctt actgcttcct ttacccattc ctcacgctcc ataaatgaca
     4081 aaaccgcccg caggcggttt cacataaaac attttgcatc agcgaccaat caccacaacc
     4141 tgaccaccgt ccccttcgtc tgccgtgctg atctcctgag aaaccacgcg tgaccccacg
     4201 cgcatttccc cgtacagaac aggcagaaca ttgccctggg caaccatgtt atccagtgag
     4261 gagaaatagg tgttctgctt accgttatcc gttgtctgtg tacggggagt tctggctttc
     4321 ggtgccagca tctgcgccac accaccgagc accatactgg caccgagaga aaacaggatg
     4381 ccggtcatac caccggcccc aatggctgcc ccccatgctg caagggtggc tccggcggta
     4441 aagaatgatc cggcaatggc ggcagccccc aggacaatct ggaatacgcc acctgacttg
     4501 gccccggcga ctctgggaac aatatgaatc acagcgccat caggcagagt ctcatgtaac
     4561 tgcgccgtta acccggacgt gctgacgtcc cgcccggcaa tccgtacctg ataccagccg
     4621 tcgctcagtt tctgacgaaa cgccgggagc tgtgtggcca gtgcccggat ggcttcagcc
     4681 cccgttttca cacgaaggtc gatgcggcga ccaaatcgtt gtaaatcccc gtaaaggcag
     4741 atgcgcgcca tgcccggtga cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtgt
     4801 acctctctcg tttgctcagt tgttcaggaa tatggtgcag cagctcgccg tcgccgcagt
     4861 aaattgcggc gtgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggct
     4921 gtgccgctga caacggcacc tgatacagcc ccgtcgcctc cagattatcc agatagagat
     4981 tctggccgtt acgccaccag tcatcctcac gatgaaagtc cggcatctca atccccgcca
     5041 gatgataagc atcccggaac agtgtgtaac agtccgtcac accgtgctca aagcgccgcc
     5101 cggtgagatg cggcacacag cggaacttat gaatcgtccc ccggcagacc agccaccacg
     5161 gcaaatcact ctgcacctgc agccgccggt cggcctcact cagccagggc agaccaccgg
     5221 ggtggctgtg gaccagcgcc acaatctcac cctgcatttc tgcctgcagc cagtcttccg
     5281 gcgacatacg gaaatacgcc tccggctcac cggagatatt cacgcagggg aaatatcttt
     5341 ccccctccgg cgtgcttacc acgaagccgc acgactccgc tggcgcacat cgccgggcgt
     5401 gcgccagaat cgctgattct gtctgtgtca tgggatttac tgcgaaagtt tgttaatgga
     5461 aaggaagccg ccaaagttgc cgacgttatt gcggaactta caaccgctca ggcatttgct
     5521 gcatttatcc ttcgtgatat cggacgttgg ctggtcatat tcatccgcga cagccggacc
     5581 gctataaccg cactcgtcac cgcgataggt ccaggtgcag gtgttggcca gcatgatacg
     5641 tcccggaaaa acagcgccat ccgtttccgt cggcgtggac agtacaaagg aggcactcac
     5701 cgcgctcagt tcgctgcact gctcaatgcg ccagcggctg atcacctcct gctccggatc
     5761 ggcataactg tttccgttga cgaagttcac cgcatccaga aaacgggcgt aaaccttacg
     5821 ccggaccacc gttccgccga ccagactctg cagatcttcc gccatcccgg tgaccatacc
     5881 gtacaggtta gaaaccgtca gcgtggggcg cgtactggtg cctttgccat tcagttcaaa
     5941 acc
//
LOCUS       DAZFZC010000187         5911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFZC010000187 DAZFZC010000000
VERSION     DAZFZC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.189"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..266
                     /locus_tag="ACHKXP_004888"
     CDS             <1..266
                     /locus_tag="ACHKXP_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7649705.1"
                     /translation="APWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFG
                     QVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(314..526)
                     /locus_tag="ACHKXP_004889"
                     /pseudo
     CDS             complement(314..526)
                     /locus_tag="ACHKXP_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            588..761
                     /locus_tag="ACHKXP_004890"
                     /pseudo
     CDS             588..761
                     /locus_tag="ACHKXP_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1204..5106
                     /gene="espP"
                     /locus_tag="ACHKXP_004891"
     CDS             1204..5106
                     /gene="espP"
                     /locus_tag="ACHKXP_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO7649706.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASRMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(5354..5611)
                     /locus_tag="ACHKXP_004892"
     CDS             complement(5354..5611)
                     /locus_tag="ACHKXP_004892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649707.1"
                     /translation="MSVKLRLPHTAQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCF
                     MKTNAPRLFFVDKESGVSFDVFHVLSVSFPVTACATHDGGM"
     gene            5650..>5911
                     /locus_tag="ACHKXP_004893"
     CDS             5650..>5911
                     /locus_tag="ACHKXP_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7649708.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILKKA"
BASE COUNT         1743 a         1354 c         1347 g         1467 t
ORIGIN      
        1 ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca ttcatcgggt
       61 gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca
      121 cagaccgctt tgggcaggtt cattacctgt tgctggagcc ggaggccggg aaagaattca
      181 gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac
      241 tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca ctatgtttaa
      301 tcttccggga tggttgtagt tgcagaattt cagattccag ttcagccacc gtgcgggaac
      361 caggagtacc gagccctttt ctggcggcgg taacccattg tcctaaagtg ccttcaggaa
      421 gagataatcg ggaagcgcct tcactgatcg aaagttgatt ttcaagaacc gttctgacag
      481 cttcggcttt gaactcttta gagtaacgtt gggtttttct gctcattatt agctccttct
      541 gatgccattc tatttcagga aggagtgtcc gttaaactca ggctacctca gaccatggtg
      601 gtgagcgggg agcgctactg tacagcctga tcgggacgtg caaactgaat gacgtggatc
      661 cagaaagcta ccttcgccat gtgcctggcg tcatagcaga ctggccggtc aaccgggtca
      721 gcgaactgct tccgtggcgc atagcactgc cagctgaata acacatcccc gtcaatacgg
      781 ccctcgctgt acgcttacgc ataaatattg aatacattta ctgagcatgc aaaagaaaaa
      841 gacaaactaa tcccggcctt tcacagaatt tcctctatca gaggctgagt tgacaacctg
      901 ctcacccagg aaatagtaac acccgaaaac atgaagagac tctgataaac aatctcgata
      961 catattcctc atctttcaca gcatctcact tctttaacag gaattattat tatctacgcc
     1021 tcctcctttc atgtatccct catgaatgat taagataaac atactgtgtt tgttatccgt
     1081 atgataacaa acacataaag gagtaaacat ttatctggat gatgattaca atgttcacga
     1141 ctgtcagaga cacacctctg atacattaga aaaaccaatg tttcccttaa aaatggagct
     1201 tatatgaata aaatatactc tcttaaatac agccatatta caggagggtt aatcgctgtt
     1261 tctgaattat ccggcagagt atcatcaaga gcaactggta agaaaaaaca caaacgcata
     1321 cttgcattat gttttttagg cttattacaa tcctcatatt cttttgcgtc acggatggat
     1381 atttcaaatt tctacatccg tgactatatg gattttgcac agaacaaggg catatttcag
     1441 gctggcgcaa caaatattga aatagtgaag aaagatggct ccaccctgaa actaccggaa
     1501 gtaccatttc ctgacttctc accggttgca aacaaagggt caaccacatc tattggtggt
     1561 gcatacagta tcacagccac acacaatacg aaaaaccacc actcagttgc gacgcaaaac
     1621 tgggggaaca gcacgtacaa acaaactgac tggaatactt cacatcctga ttttgcagta
     1681 tcccgacttg acaagtttgt tgttgagacc cgaggtgcga ctgaaggcgc agatatttcg
     1741 ttatcaaaac agcaggcact tgaacgttac ggggttaatt ataaaggaga aaagaaactt
     1801 atcgcattca gagccggctc tggtgtggta tccgttaaaa aaaatggacg cataactcca
     1861 tttaatgagg tttcttataa gccagaaatg ttaaatggct ctttcgttca cattgatgac
     1921 tggagtggat ggctgatatt aaccaacaac cagtttgatg agtttaataa cattgcctct
     1981 cagggtgaca gcggttcagc actgttcgtc tatgataacc aaaagaaaaa gtgggttgtc
     2041 gctggaactg tctgggggat ttataattac gccaatggca aaaaccacgc agcatacagt
     2101 aaatggaacc agacaaccat tgacaacctg aagaacaagt attcttacaa cgtggatatg
     2161 tcaggagctc aggttgcaac cattgaaaat ggaaaactga caggcactgg ctcagacacc
     2221 accgatataa aaaataagga cttaatattt actggcggtg gagatatcct cctgaaatcc
     2281 tcttttgata atggtgctgg cggtcttgtc tttaatgata aaaagaccta tcgagtaaac
     2341 ggggatgatt tcacctttaa aggtgccggt gttgatacaa gaaacggcag caccgttgag
     2401 tggaatatcc ggtatgataa taaagacaac cttcacaaaa ttggtgatgg cacattagat
     2461 gtccgaaaaa cccagaacac caacctgaaa acaggtgagg gtcttgtcat tcttggagct
     2521 gaaaaaacat tcaataatat ctacataacc agtggtgatg gaactgtccg actgaatgca
     2581 gaaaatgcac tgtctggcgg tgaatacaac ggtattttct ttgcgaaaaa tggcggaact
     2641 cttgacctga acggatataa tcagtctttc aataaaattg ctgcaactga ttcaggtgct
     2701 gtaataacca atacgtcaac caaaaaatcc attttatccc tgaataatac tgctgactat
     2761 atctatcacg gcaacataaa cgggaatctg gacgtacttc agcatcatga gacgaaaaaa
     2821 gagaaccgtc gtcttattct tgatgggggc gtggacacaa caaatgatat aagcctgcgt
     2881 aatacacaac tgtccatgca gggacatgcc actgaacatg ccatttatcg ggatggagct
     2941 ttctcttgtt cactaccagc tcctatgcgc tttttgtgtg gcagtgatta tgttgcagga
     3001 atgcaaaata cagaagctga tgctgtaaaa caaaacggaa atgcctataa aaccaacaat
     3061 gctgtctctg atttatcgca gccagactgg gaaaccggaa cattcagatt tggaacgcta
     3121 catcttgaaa attccgattt ttctgttggt cgtaatgcaa atgtaatcgg ggacattcag
     3181 gccagtaaat caaacattac tattggtgac actacagcat atattgattt gcatgctggt
     3241 aaaaatatta ccggtgatgg ttttggcttc cgccagaata ttgtgcgtgg aaactcacaa
     3301 ggagaaacgc tgtttacagg agggatcaca gcagaagaca gcactatcgt tattaaagat
     3361 aaagcaaaag cattattttc aaattatgta tacctgctga acacaaaagc aaccatagag
     3421 aacggtgctg atgtgacaac tcaaagtggt atgttctcca cgagcgatat cagcatctct
     3481 ggtaatctgt ccatgacagg caatcccgac aaagacaata aattcgagcc ctcaatatat
     3541 ctgaatgatg cttcttatct actgactgac gactccgcca gactcgttgc caaaaataaa
     3601 gcatctgtgg tgggagatat acactccact aaaagtgcat ccatcatgtt tggtcatgat
     3661 gaaagcgacc tctcgcagtt gtctgacaga acctcaaaag ggcttgcact tggtctttta
     3721 ggtggctttg atgtctcata tcgcggttca gtcaatgccc cgtcagcatc tgccactatg
     3781 aacaatacct ggtggcaact aaccggagat tctgcgctga aaacactgaa aagtacaaac
     3841 agcatggtct atttcactga cagcgcaaac aataagaaat tccatacgct gacggtcgat
     3901 gagctggcaa ccagcaacag cgcctatgcg atgcgtacaa acctttctga atcagacaaa
     3961 ctggaggtca aaaaacacct gtctggtgag aacaatattt tactcgttga tttccttcag
     4021 aaaccaacgc ctgaaaaaca actgaatatt gaactggtaa gcgcgccaaa agacaccaat
     4081 gaaaatgtct ttaaagccag taaacaaacc attggtttca gtgatgtaac gccggtcatt
     4141 acaaccaggg aaaccgatga caaaataaca tggtcactga caggctataa cacggtagca
     4201 aacaaggaag caacccggaa tgccgccgcc ctgttctctg ttgactataa agcgtttctg
     4261 aacgaggtca acaacctgaa caaacgtatg ggtgacctgc gtgatatcaa cggcgaagcc
     4321 ggtgcatggg cacgcatcat gagcggtacc ggctctgcca gtggtggttt cagtgacaac
     4381 tacacgcacg ttcaggtcgg ggtcgacaaa aaacacgagc tggacggact ggatttgttt
     4441 accggtttca ctgtcacaca cactgacagc agtgcctccg ccgatgtttt cagtggtaaa
     4501 acgaagtctg tgggggctgg cctgtatgct tccgccatgt ttgattccgg tgcctatatc
     4561 gacctgattg gcaagtatgt tcaccatgat aatgagtaca ctgcaacctt tgccggactc
     4621 ggaacccgtg attacagcac gcattcatgg tatgccggtg cagaagcggg ctaccgctat
     4681 catgtcactg aggatgcctg gattgagcca caggctgagc tggtttacgg ttctgtatcc
     4741 ggtaaacagt ttgcatggaa ggaccaggga atgcatctgt ccatgaagga caaggactac
     4801 aatccgctga ttggccgaac gggtgtggat gtgggtaaat ccttctctgg taaggactgg
     4861 aaagtgacag cccgtgccgg tctgggctac cagttcgacc tgctggctaa cggcgaaacc
     4921 gtattgcggg atgcatctgg tgaaaaacgc atcaaaggtg aaaaggacag ccgtatgctg
     4981 atgtccgttg gcctgaatgc agaaatcagg gataacgtcc gctttggact ggagtttgag
     5041 aaatccgcct ttggtaagta caacgttgat aatgcagtca acgctaattt ccgttactcg
     5101 ttctgatact ccgtattcaa aagggcctgc gggcccttta cgctcagtgc ctgccgccct
     5161 gatatgtctg agagacattt ccgggatgtc agtcgactgg tttacttaac tgcgatgact
     5221 aaagctggca gtaatgccgg cttttttacg gccacatacg tcttcctctc atccggaact
     5281 gtgatacaaa tcctgcatac aggattgacc gccggttcct gcaaatggca cattttcagt
     5341 aagatagcga aggttacatt cccccgtcat gtgtggcaca agctgtcacc gggaaagaaa
     5401 cggaaagcac atgaaaaaca tcaaaactca caccggactc cttatccaca aagaacaaac
     5461 gcggcgcgtt cgtcttcatg aaacacccac tgcctggtgc cacacgcacc gggaatgcta
     5521 cagcaaaacg accggtcgcc gctgcggcag ccctgacagt ctcagcaggc tgattctgag
     5581 cagtatgagg tagcctgagt ttaacggaca ctccttcctg aaatagaatg gcatcagaag
     5641 gagctaataa tgagcagaaa aacccaacgt tactctaaag agttcaaagc cgaagctgtc
     5701 agaacggttc ttgaaaatca actttcgatc agtgaaggcg cttcccgatt atctcttcct
     5761 gaaggcactt taggacaatg ggttaccgcc gccagaaaag ggctcggtac tcctggttcc
     5821 cgcacggtgg ctgaactgga atctgaaatt ctgcaactgc gtaaggcgtt aaatgaagct
     5881 cgccttgagc gagatatatt aaaaaaagca a
//
LOCUS       DAZFZC010000188         5785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFZC010000188 DAZFZC010000000
VERSION     DAZFZC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.190"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..655)
                     /locus_tag="ACHKXP_004894"
                     /pseudo
     CDS             complement(1..655)
                     /locus_tag="ACHKXP_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(725..904)
                     /locus_tag="ACHKXP_004895"
     CDS             complement(725..904)
                     /locus_tag="ACHKXP_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649709.1"
                     /translation="MLPAPPAHLQSVFVVIILSHDEWLKGILSLLNWHEHHQWMKATD
                     RDVLYSQLHATFSTD"
     gene            complement(945..1553)
                     /locus_tag="ACHKXP_004896"
     CDS             complement(945..1553)
                     /locus_tag="ACHKXP_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649710.1"
                     /translation="MKMKQQYQTRYESLHESYQKWLTGFTRHAVSWGVCHPNIYYFHN
                     LTPGWISFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLVVNLCTSEHLLVHHPML
                     EGILLSECERLKHHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWTENLK
                     RKSEKELEEWIIDRQKQNAALTDLMDQYVLLAYRTTVDDNRN"
     gene            complement(1642..1848)
                     /locus_tag="ACHKXP_004897"
     CDS             complement(1642..1848)
                     /locus_tag="ACHKXP_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7649711.1"
                     /translation="MNTPVSLMDDQLVDMAFITQLTGLTDKWFYKLIRDGAFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2054..2238
                     /locus_tag="ACHKXP_004898"
                     /pseudo
     CDS             2054..2238
                     /locus_tag="ACHKXP_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2563..2685
                     /locus_tag="ACHKXP_004899"
                     /pseudo
     CDS             2563..2685
                     /locus_tag="ACHKXP_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(3000..3194)
                     /locus_tag="ACHKXP_004900"
     CDS             complement(3000..3194)
                     /locus_tag="ACHKXP_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649712.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIFTAL
                     LALLVVVLLVTSAVSMYVLW"
     gene            3394..3963
                     /locus_tag="ACHKXP_004901"
     CDS             3394..3963
                     /locus_tag="ACHKXP_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7649713.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERDDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            4131..4514
                     /locus_tag="ACHKXP_004902"
     CDS             4131..4514
                     /locus_tag="ACHKXP_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7649714.1"
                     /translation="MYAKSFLALDGNGRLTGARTAHTAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERKEIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            4511..4936
                     /locus_tag="ACHKXP_004903"
     CDS             4511..4936
                     /locus_tag="ACHKXP_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7649715.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            5223..5378
                     /locus_tag="ACHKXP_004904"
                     /pseudo
     CDS             5223..5378
                     /locus_tag="ACHKXP_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(5627..5785)
                     /locus_tag="ACHKXP_004905"
                     /pseudo
     CDS             complement(5627..5785)
                     /locus_tag="ACHKXP_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1392 a         1444 c         1539 g         1410 t
ORIGIN      
        1 gtaagtaaaa tcagccaccc acaactggtc aggacgttct gccccgaact gacggtttac
       61 gcggtcgcct gcggtaatgg ctttccggct gacggtagtg cggacctttt taccccggag
      121 aacaccggca agccccataa ccgccatgag acgtgccaca gtgcacctgg ccactctgat
      181 accttcccgt aacaactgac gccagacttt acgtacactg tatacctgat gattctcatc
      241 gtatacgcgc agtatctctt tcttcagcca gtcatcgcgc tgcgcacggg tactgcgttt
      301 atcaggacga tgtcgctgtt gctgacagtg gtaatacgtt gacggggcaa tatgcagttc
      361 actgcatata agcggaagtc tggcgaagga tatcgttact gcggcgcagt tcacgatttt
      421 tacgctccag ctctttcaga ggctgacgtt cagtggtggt gagcccacca tcaccgcctc
      481 cggtatcccg ctcatgctgg cgtacccaga cacgcagagt ctccggcgta cagccaatct
      541 ttggggtaat ggaacaaatt gccgcccatt gtgagtcata ttcgccctga ctttccagaa
      601 ccatacgaac tgcccgttga cggacttcgg gggaaaaacg tgtattttta gtcatcctgt
      661 ttacctcttt ctcagggagt ttagtctcca ggatttccgg ggcggttcac ctctgcctgc
      721 atattcagtc cgtagagaac gtggcgtgta gctggctgta aagaacatcc ctgtccgtgg
      781 ctttcatcca ctggtggtgt tcatgccagt tcagtaatga caggatgccc ttaagccatt
      841 catcatggct cagtatgatg acgacgaaca cgctctgcag gtgagcaggc ggggccggga
      901 gcataattcc cgtattctgc cgcaccttcc ggaagcaacc ggcatcagtt acggttatcg
      961 tcaaccgttg tgcggtatgc caggagcacg tactgatcca tcagatccgt cagtgctgcg
     1021 ttctgcttct gacggtcgat gatccattcc tccagctctt tttctgattt ccgtttcagg
     1081 ttctctgtcc agtcttcaag gctgtttccg gtcattaaat caagccagca aagccagacc
     1141 agtttgaggc gcaactctgt gccaccaaac tgacggaaga ggctgatcag tttatttgcc
     1201 agagaatgat gtttcagacg ttcacattca gacagcagaa tgccttccag catcggatga
     1261 tgaaccagca gatgttcact ggtgcataag ttcaccacca gatcatcatc cagcggcggc
     1321 gtggtccgtt tgtcagggcc ataaatcagc cggtgcagac tctggggtac gatggcgatc
     1381 tcgggttttt caccattgaa tgatatccat ccgggcgtca gattatggaa gtagtagata
     1441 ttcggatgac acactcccca ggatacagcg tgccgggtga agccggtcag ccatttctgg
     1501 tagctttcgt ggagcgattc gtagcgggtc tgatattgtt gtttcatttt catcttcctc
     1561 tcggggatat aacaggcagc agaatgccgc cgacaggccg gaaattgcgt ctgctgcagg
     1621 taaacggata acggttatga actacggacg ggactgtgca atgcgtgcct gcagccaggc
     1681 ttccacttcg cttttcagcc agcgggagct gcgacccatt ttgatagggg ccggaaaggc
     1741 accatctctg atgagcttat aaaaccactt atcggttaag ccggtcagtt gagtgataaa
     1801 tgccatgtcg accagctggt catccatcag cgaaaccggg gtattcatga aaatctcctt
     1861 cggatgttgt taatctcaac cgacgtttgt gatgaaaaca acgcatcacc tgcaccggca
     1921 taacaaccgg ggagaaacgt aaaaaatgag taggggaatg ttcctgccgg gagataaaca
     1981 gggagcatga tgctgagata cccggtggct accgcgaaac gatgcttctg cacgcacgcg
     2041 cgataaagaa gggatgattt tcgtgaggat gggggaaaaa ccggcgttct gataattgca
     2101 gagttgtttt gggaggcggt tacagtaagt cagtctgttc ctagcgggca ggcgacaggt
     2161 atcaccgatg ggataagact gtctgattgg tattataaat atataagaga gatcaaacac
     2221 atgagttcat atctgtgaaa taaacactgt gagtgtgatc agtgggcatg agaacgcagt
     2281 agcaacagat tacgttgtac tcacagggct atccctgata gctggcgtat aacctgcgta
     2341 tataaaacgt acctgcaaca cacgtgaaca gagtgcatta agcatccgtc tgagcggtaa
     2401 acgtatccga cctggtgagt gacagtctga gagaccggtt aagtggtggt acgggagtaa
     2461 tgagtttggg gtgggtacca gaatgccagt ctggtgcggt atgctggcag tatcacgtta
     2521 attgcagcat aaagtgatga gaggctaagt gatgagaggc taatggaagt taaaaccaaa
     2581 gaagactggc tgtatcagtt ccgtcgttgt tcatcccggg agacactgga aaaagtgatt
     2641 acccgcacac gttataaact tacccctgcg gagctggaag ccttctgcgg tcgatcggct
     2701 ggcagaactg acgacaaact ttacgcgtgt ggagccagtc tggtcacctg agtatccggt
     2761 aatgacaacg gtctggtgtt atctgctctg tgtgattctt tttaggtctt ctccggttgt
     2821 tggtaacctg gataataccg cgaacgttaa gcggtattgt gacaacgaat aatttgaaat
     2881 taaaaggggc aggggggggc tgcttcgggt attaacggct tacagcgtgt gggttgacgg
     2941 cgcgttgcag gggacgaact ctggcaaccc tcacggatat ctgataagcc ccgttaccgc
     3001 taccacagca catacatgct gacagcagat gtcaccagca aaaccacaac cagcagcgcc
     3061 agcagggctg tgaagatcct gtccgtattc cggtaatagc cgaaggggtc gacagaacca
     3121 cagtacggac aacacaccgt attatgagta atcgcgttac cacagtccct gcacttcttt
     3181 ttgcgtatca ccatcactcc ctcctcacag ccttacccgt aaccggtttt ttacattaaa
     3241 aaaacaacgc tgaaaattca accgtcagtc cggagacgac cgttcgggtt atcacaaagt
     3301 gcctgagaca gtgtcctgcc ggaggtcact tcagggactc tgcgcatttt ttacgacgtg
     3361 gctattccgt atgaaccata cggagattta accatgacct acaaatacaa ccccttctgg
     3421 cagcaacgta ttcgtgagac ggtgcggcac gcactgaatg ttcatccccg cctgacggca
     3481 ttgcgggttg atctgcgttt cccggatgta ccggcagcaa cggacgcagc tgtgatatcc
     3541 cgcttcatca atgccctgaa agcccgaatc gacgcttacc agaaacgtaa gcatcgggaa
     3601 ggtaaacgcg tgcatcccac cactctgcat tacgtctggg cccgggagtt tggggagtgc
     3661 aaaggtaaaa aacactatca tctgatgctg ctggtcaacc gggatacctg gtgtcgtgcc
     3721 ggtgattacc gcgctccggg atcactggcc gggatgatta aacaggcgtg gtgcagtgcc
     3781 ctgggagtgg ataccggaga tcataccacg ctggcgcatt ttccggtcag gccggcggtg
     3841 tggctggaac gcgatgatga caccggcttt cagcaggtgc tggagcgtgc tgactatctg
     3901 gcgaaggaga gtaccaaagc ttacggcacc ggtgagcgca actttggctg cagtcgtggc
     3961 tgagcccgac agacgactta tctgacctta gtgtgatgac gtgttcacag cgaccacact
     4021 gattttccct tctgagactg aactgaaaac cgccattcac acctgaccac gctgcccgta
     4081 ccgggacgac tgttgcctgc gtgtcgtctg gcggctgagg aatatatact atgtacgcaa
     4141 aatcctttct cgcacttgat ggtaacggac gtctgacggg ggcccgtacc gcacatactg
     4201 ctccttatgc ccactacacc tgccacttgt gtggcagtgc actcagatac cacccgcaat
     4261 acgacaccga actgccctgg tttgaacaca ctgacgacgg gctgacagag cacggtcaac
     4321 agtgtcctta tgtcagaccg gagcgcaagg agatacaact gattaaacgt ctgcagcaat
     4381 tcgtaccgga tgccttaccc gtagtacgta aagccagctg gcactgcagg caatgtcacc
     4441 acgattatta tggggagcgg tactgcacac actgccagac cgggcgcttc agtgaggagg
     4501 ggggagcaga atgaacggta taaaaacggg gctggggatc acgccgggtg agcacattat
     4561 tagtgccgac tctgcgctca gcagaaatat ccggcagtgt ttctgccttt cctgtcatgg
     4621 ccggttgatt ctgcagacag atgctcaggg ggcgtggttt gagcacgatt tacatgccct
     4681 gagcgagcaa cagaaggcgg actgtgtggt gctgaatccg gagaagtccc acccttacat
     4741 tgaggatatg gcgatgtttt tatcgccatt accggtcgtg ctggagtggc attgtgtgat
     4801 gtgtgagcag ttttttcatg ggaagaagtt ctgtgaggcc tgtggtacgg ggatttattg
     4861 cagagcggta tgtacgaagt ctgtttacag ctaccagcca gacttgtttg aggactgcgg
     4921 aggtgcgagt acttaatccg aaatctccgc tctgcgcagg gcgttcagag gtatcgcaac
     4981 ctgcatttta tccggtgttt cgcacctgcc ccgttcgtga cacaaaggcg cgacaccgga
     5041 tatccgcagt cctgcttacc tgaataaaac ccggggtatt gattggtagt gtggtgcgga
     5101 atcgtaccgt gatctcgcat tcgccgaatt cgcactgtgg tgtcaatcaa ccatacgcga
     5161 gagtcctctc gtatttatca gtgcctcagg acgatttctg catagttaat ccagggggct
     5221 gcattgttca tttccctgct aatggacgtg acatcctgga tattaacgat cctgatttcc
     5281 ctgagcagta tcagcgctta ttggcacgtc tggattatct gacgaagctc gacactaaag
     5341 catcaggcca gcgtaactct ggctacagcg gtttttgaca gttgtaactg taaggtgatt
     5401 tattatgtat acagaatcaa atgttaagct ttcctgtaac gccctctggc aaaagtgtgt
     5461 ccttgcaact ctgtatcggg ctgcagctac acatcctgaa attaaattat tacaatttaa
     5521 attaatacca gggcagggca ttaataaggc cattccctct ctgatggctg attttactgg
     5581 tgaactgcat agactcattt ctgaatattt gcagtgcaag acagtccggg ctgagaccgc
     5641 cacaggcact gtgacgacgc cagcgattgt aatcacactc gatataatta aacaccgttt
     5701 cccgcattat ttcccggctg ctaaagcgtt ccccgtggat acattccacc ttcagcgaat
     5761 gaaagaaggt aagcgtcaac ggagc
//
LOCUS       DAZFZC010000189         5771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFZC010000189 DAZFZC010000000
VERSION     DAZFZC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.191"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..242)
                     /locus_tag="ACHKXP_004906"
     CDS             complement(<1..242)
                     /locus_tag="ACHKXP_004906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649716.1"
                     /translation="MVIYTDTLLHYSPETTIFRRFFAIFDKKYQLSRFALSPYITGNS
                     GDLLASIFMPHLPSGVASDSQYVRVEKSDYFHWVIYR"
     gene            complement(323..1282)
                     /gene="nrdF"
                     /locus_tag="ACHKXP_004907"
     CDS             complement(323..1282)
                     /gene="nrdF"
                     /locus_tag="ACHKXP_004907"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7649717.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1292..3436)
                     /gene="nrdE"
                     /locus_tag="ACHKXP_004908"
     CDS             complement(1292..3436)
                     /gene="nrdE"
                     /locus_tag="ACHKXP_004908"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7649718.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLTLQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTRTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3409..3819)
                     /gene="nrdI"
                     /locus_tag="ACHKXP_004909"
     CDS             complement(3409..3819)
                     /gene="nrdI"
                     /locus_tag="ACHKXP_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO7649719.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3816..4061)
                     /gene="nrdH"
                     /locus_tag="ACHKXP_004910"
     CDS             complement(3816..4061)
                     /gene="nrdH"
                     /locus_tag="ACHKXP_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO7649720.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4309..4638)
                     /locus_tag="ACHKXP_004911"
     CDS             complement(4309..4638)
                     /locus_tag="ACHKXP_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO7649721.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4790..5134
                     /locus_tag="ACHKXP_004912"
     CDS             4790..5134
                     /locus_tag="ACHKXP_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO7649722.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5171..5620)
                     /gene="alaE"
                     /locus_tag="ACHKXP_004913"
     CDS             complement(5171..5620)
                     /gene="alaE"
                     /locus_tag="ACHKXP_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO7649723.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1479 a         1419 c         1431 g         1442 t
ORIGIN      
        1 cgatatatta cccaatggaa atagtcactt ttttctaccc taacatactg agaatctgag
       61 gcaacccctg atggcaaatg tggcatgaat attgatgcga gcaaatcgcc ggaatttccc
      121 gtgatataag ggctgagagc aaatcgagaa agttgatatt ttttgtcaaa gatagcgaaa
      181 aaacgcctga atattgtggt ttcaggcgaa taatgcaaca gagtatcagt gtagatcacc
      241 acaaattatt tgcgtgataa aaagctattt ggcgggaatt atttccctgc tgcgggtagt
      301 gatatttttg aaaataacac cctcagaaat tccagtcttc atcttctgtt tcaaccgctt
      361 tccccatcac ataagaggag cctgaaccgg aaaagaaatc gtgattttca tcggcattcg
      421 gcgaaagcgc ggcgaggatt gccggattca cttccgccat ttctgcggga aataacggtt
      481 cgtagcccag attcatcaaa gccttattgg cgttgtaaca gagaaacgct ttcacatcgt
      541 cagcccacgg ggtttcggcg tacagctcat cggtgtattg caactcgttg tcgtagagtt
      601 ccagcaacaa atcgaaggcg aaactcttca actcttcacg ttgtcccaga gatatctttt
      661 ccatgttttt ctgatattta tagcctatgt agtaaccgtg gactgcttca tcgcggataa
      721 tcagacggat caggtccgcg gtattggtca gctttccgcg gctggaaaaa tacatcggca
      781 gccagaaacc ggaatagaac aaaaaagatt caagaaacac actggcgatt ttctttttca
      841 gcggatcatc accgcgataa tgttgctgaa taatctgagc ttttcgctgc aacggtgcgt
      901 tttcttcact ccaggcgtag gcggcatcga catctttggt ctggcatagc gtcgagaaaa
      961 tcgaactgta agagcgggca tgaaccgctt ccataaagct gatattcgat aataccgctt
     1021 cttcatgagg cgtgagtgca tcgggcatca gagaaggcgc gccgataaca ttttgcagcg
     1081 tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag ttgttgttct acgacagtta
     1141 atgtctgcca ggcaggaata tcgttcgaca gcggcacctt ttctggtagc cagaaattgc
     1201 tggtcaggcg attccacacc tccagatctt tatcgtcaga tatcttgttc cagttgatgg
     1261 cgctgatacg tgagagtttc atagatattc cttaaagtgc acaggagacg cagccttcaa
     1321 tttcagtgcc ttccagcgcc atctgacgca ggcggatgta atagagcgtt ttgataccct
     1381 tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt ggtggcggta tcggggaaaa
     1441 aaagcgtcag cgacagcccc tgatcgacat ggcgagtcgc ttccgcgtag gtgtcgatga
     1501 tcttttctgc gccaatttcg taagcgtcct gatacagcgc cagattctcg ttagtcataa
     1561 acggggcagg gtagtaaacg cgtcctgttt tgccctcttt gcgtatctct actttcgcca
     1621 caatcggatg aatactcgac gtagcatggt tgatataaga gatagaaccg gttggcggca
     1681 ccgcctgaag attctggttg tatatgccgt agcgcatcac gtcgtcgcgc agctgcgccc
     1741 acatctcacg ggtaggtaac gtaataccgc tacgggtaaa cagttcgcca actttcgccg
     1801 ttttcggctg ccagttccct tgcagatatt ggctaaaata ttcaccactg gcatagcgtg
     1861 actgtttgaa cccggcgaag gtttcaccgc gttcgcgtgc cagcaacatc gaggtacgca
     1921 gtgcatgcca ggtgatggca tagaaataga gattggtgaa atccagtgct tccggcgaac
     1981 cataagcgat gccttctcgc gccagatagc cgtgtaaatt catctgcccc agtccgatgg
     2041 cgtgcgaggc ggcatttccg gcttcgatgg acggcacgct gcggatatga ctcatatctg
     2101 atactgccgt taaaccgcgc acggcagtct ctaccgtgcg ggcaaaatcg ggggaatcca
     2161 tggtgtgcgc aatattcaac gaacctaaat tgcaggaaat atcatggcct gtgcgggtat
     2221 agtcgagatt ctcgtcatac tctgaggcgc tgttaacctg caaaatttct gagcagagat
     2281 tactcatatt tatgcgcccg gcgatagggt tagcacggtt taccgtgtct tcatacatga
     2341 tgtagggata gccggactca aactggattt ctgccagtcg ctggaagaaa tcacgggcgt
     2401 tgaggtattt tttgcgaatg cgttcatcgg caaccagttc gtcatagtgt tggctgatgg
     2461 ccacatcggc aaacggcttg ccataaactc gctctacgtc ataaggcgaa aacagcgcca
     2521 tctgcgcatt ctcttttgcc agatggaaag tgatatccgg gatcaccacg ccaagcgaca
     2581 gtgttttaat gcggattttt tcgtcggcat tttcccgttt cgtgtcgaga aaacgcagaa
     2641 tatcgggatg atgagcatgt aaatagactg caccagcccc ctgacgagcg ccgagttggt
     2701 tggcatagga aaatgcgtct tccagcattt tcatcaccgg aattacgcca gaagattgat
     2761 tttcaatacg tttaattggc gcgcccgctt ctcgcagatt cgacagcaaa aatgctacgc
     2821 cgccgccgcg tttcgacagc tgcagtgcgg aatttaccgc ccgaccaatc gactccatat
     2881 tgtcttcaat acgcagcaaa aaacaggaaa ccagttcgcc gcgctgctgc ttaccgcagt
     2941 tgaggaatgt tggcgtggct ggctgaaagc gtcctgacag catttcatcg gtcagttgca
     3001 acgtcagcgt ctcatcgcct tgtgccagcg tcagcgccac catcgttact cgatcggcaa
     3061 aatcttccag ataacgttta ccgtcgaatg tcttcaacgt atagctggtg taaaacttcc
     3121 atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg tgcgtgggca aacagcgtaa
     3181 tgacaaagtc gcgagagtag cgattaagaa cgctttcatc atagtaacct tcgttgacca
     3241 gccagttcag gcgctgctgc tggctactga aggtcacact gttcggacgc acatgcgtcg
     3301 caataaaggc gtcaacggcc tggcggtctt tatcgaactg aatgcgacct gcgctatcgt
     3361 agaggttaag catcgcattc agcgcgtggt aatccatcgt ttcctgcgtc aggcattctg
     3421 cggttgtcgt tgccaaaatt cggttactcc tttacgaacg ttttcgatat cgctttgcgt
     3481 acccatgagt tcaaaacggt acagccacgg cacgccgcat ttccgggcaa tcacatctcc
     3541 ggcgcggcca tacgcctcac caaagttgcg attaccagaa gcaataacgc cgcgaagcaa
     3601 cgcccggttg tgctcgtcgt ttaaaaagcg aattacctgt cgtggcaccg cgccagccgt
     3661 accgccgccg ccgtaagagg gcacgatcag gatgtaaggc tcgtctacct gaatccgttc
     3721 ccgctcattg agcgggatgc gcaccgcggg cagacctaaa cgttcgataa aacgctgcgt
     3781 gttttcggag ctgctggaga agtagacgag ctggctcatg cactggccgc gtgtggcgct
     3841 ggatgcagac ggttaatcat gtccggacgg aaaccagacc agctaagatc gccagcaatc
     3901 actaccggca actgacgaaa gccctgagca cgcaacgctt ctgccgcttc aggaacgcga
     3961 tcgacattaa tcatttcaaa atcaaagccc cggttttcca tcgcccgttt ggtggcgtgg
     4021 cactgaacgc aatcgttacg agtgtaaata gtaatgcgca tgattcgtat ttccgtttaa
     4081 aatgaagata cggcgcgatg atacgcgtcg ggttgtctct ctgttgatac agagatacta
     4141 gatgtagttg aaaaaagatt caaccacaca atatatagca aatagtggtc gaaattaccc
     4201 tggatatgag cgtgacgggg tagggggatt tttgtgattc accaggcaaa aagaaacccc
     4261 gaagacaggc ttcggggtca aagacgcgta tttattatca tttttgcact acgatttgcg
     4321 catgcttaac agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca
     4381 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgcgcgg cttgctggac
     4441 gcgagtacga ccatgcattc gcgccctggt ttctttcagc aatgcctgag ctttactgcg
     4501 tgcggcttca gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgaggct
     4561 gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
     4621 gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc
     4681 ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag cgttgtgaat
     4741 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
     4801 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
     4861 gaaagcgtat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
     4921 gggacgtacc gctctggtaa ttcatccaac attaaaagaa cgcagttcga cactggctga
     4981 acccgcttcc gatattaaaa cttgcgatca ttaccagcaa tttccgctct atttagcagg
     5041 cgagcgacac gagcattacg gtatcccgca tggctttagt tcgcgtgttg cgcttgaacg
     5101 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
     5161 aaaagaagag tcaggctttt acctgctggt aacggctgac tttaaacagt cggcggcaat
     5221 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
     5281 ccgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
     5341 gtgactggaa cgtcacataa gccaggatat ccgccagatt ttttatccag cccgacgggc
     5401 taactttgcg tgccgcgcgc ataaacagat cacggtacat accgtatggc catgcaatta
     5461 agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
     5521 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
     5581 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
     5641 gcgtaatggg atgttaatgg agatgaaagc agtggatctc attgccatct gcattttcgc
     5701 atagcgtata tcgctggcta tgactaatca attagtgaaa aatttttatt tagtcagttt
     5761 ttgtagataa a
//
LOCUS       DAZFZC010000190         5765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFZC010000190 DAZFZC010000000
VERSION     DAZFZC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.192"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..330
                     /locus_tag="ACHKXP_004914"
     CDS             <1..330
                     /locus_tag="ACHKXP_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7649724.1"
                     /translation="DADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRI
                     MLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFL
                     SINKLSQ"
     gene            335..1078
                     /locus_tag="ACHKXP_004915"
     CDS             335..1078
                     /locus_tag="ACHKXP_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7649725.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            1075..1647
                     /locus_tag="ACHKXP_004916"
     CDS             1075..1647
                     /locus_tag="ACHKXP_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7649726.1"
                     /translation="MKTGAEAIRALAMQIPAFRQKLSDGWYQVRISGRDVSTSGLTAQ
                     LHEVLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1708..5106
                     /locus_tag="ACHKXP_004917"
     CDS             1708..5106
                     /locus_tag="ACHKXP_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649727.1"
                     /translation="MGKGSSKGHTPREAKDNLKSSQMLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNTVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDIIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPASGTTLISLVDGQGSPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVELTENNASKLEEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPSNGNTRP
                     MFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            5173..>5765
                     /locus_tag="ACHKXP_004918"
     CDS             5173..>5765
                     /locus_tag="ACHKXP_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7649728.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLNAGYLHARTNVPS
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGY"
BASE COUNT         1315 a         1444 c         1800 g         1206 t
ORIGIN      
        1 gacgccgatc cggagcagga ggtgatcagc cgctggcgca ttgagcagtg cagcgaactg
       61 agcgcggtga gtgcctcttt tgtactgtcc acgccgacgg aaacggacgg cgctgttttt
      121 ccgggacgta tcatgctggc caacacctgc acctggacct atcgcggcga tgagtgcggt
      181 tatcacggtc cggcggtcgc ggatgaatat gaccagccaa cgtccgatat cacgaaggat
      241 aaatgcagca aatgcctgag tggctgtaag tttcgcaata acgtcggcaa ctttggcggc
      301 ttcctttcca ttaacaaact ttcgcagtaa tcccatgaca gagacagaat cagcgattct
      361 ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgcggc ttcgtggtga gaacgccgga
      421 gggggaaaga tattttccct gcgtgaatat ttccggtgag ccggaggatt atttccggat
      481 ggctccggag gactggctgc aggcagaaat gcagggtgag attgtggcgc tggtccacag
      541 ccaccccggt ggtctgccct ggctgagtga ggctgaccgg cggctgcagg tgcagagtga
      601 tttgccgtgg tggctggtct gccggggggc gattcataag ttccgctgtg tgccgcatct
      661 caccgggcgg cgctttgagc acggggtgac ggactgttac acgctgttcc gggatgctta
      721 tcatctggcg gggattgaga tgccggattt tcatcgcggg gatgactggt ggcgtcacgg
      781 tcagaatctc tatctggata atctggaggc cacagggctg tatcaggtgc cgttgtcatc
      841 agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca tcggtgccga atcatgccgc
      901 catttactgt ggtgacggcg agctgctgca ccatattcct gaacaactga gcaaacgaga
      961 gaggtatacc gacaaatggc agcgacgcac acactccctc tggcgtcacc gggcatggcg
     1021 cgcatctgcc tttacgggga tttacaacga tttggccgcc gcatcgacct tcgtgtgaaa
     1081 acgggggctg aagccattcg ggcgctggcc atgcagatcc cggcgtttcg tcagaaactg
     1141 agcgacggct ggtatcaggt acggatttcc gggcgggacg tcagcacgtc cggattgacg
     1201 gcgcagttac atgaggttct gcctgacggc gctgtgattc atattgttcc cagagtcgcc
     1261 ggggccaagt cagggggcgt attccagatt gtcctgggag cagccgccat tgccggatca
     1321 ttctttaccg ccggagccac ccttgcagca tggggggcag ccattggggc cggtggtatg
     1381 accggcatcc tgttttctct cggtgccagt atggtacttg gtggtgtggc gcagatgctg
     1441 gcaccgaaag ccagaactcc ccgtacacag acaacggata acggcaaaca aaacacgtat
     1501 ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcccgttct gtacggtgaa
     1561 atgcgcgtgg ggtcgcgggt ggtatctcag gaaatcagca cggcagacga aggggacggt
     1621 ggtcaggttg tggtgattgg tcgctgatgc aaaacatttt atgtgaaacc gcctgcgggc
     1681 ggttttgtcg tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg gcataccccg
     1741 cgcgaagcga aggacaacct gaaatcatcc cagatgctga gcgtgataga cgccatcagt
     1801 gaagggccgg ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg
     1861 gtgctggaca gtgaggggaa taccaacatc gccggtgtca cggtggtgtt ccgggctggt
     1921 gagcaggagc agactccgcc ggagggattt gaatcctccg gctccgagac ggtgctgggt
     1981 acggaagtga aatacgacac gccgatcacc cggaccatca cgtcggcaaa cattgaccga
     2041 ctgcgtttta ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg ggacaggaat
     2101 ccatcggaag tccgcctgct ggttcagata caacgtaacg gtggctgggt gacggaaaaa
     2161 gacatcacca ttaagggtaa aaccacttca cagtatctgg cctcggtggt ggtggataac
     2221 ctgccaccgc gcccgttcag tatccggatg cgcaggatga cgccggacag caccacagac
     2281 cagctgcaga acaaaacgct ctggtcgtca tacaccgaaa ttatcgatgt gaaacagtgc
     2341 tacccgaaca cggcactggt cggcgtgcag gtggactcgg agcagttcgg cagccagcag
     2401 gtgagccgta attatcatct tcgcgggcgt attctgcagg tgccgtcgaa ttataacccg
     2461 cagacgcggc aatacagcgg tatctgggac ggaacgttta aaccggcata cagcaacaac
     2521 atggcctggt gtctgtggga tatgctgacc cacccgcgct acggcatggg gaaacgtctt
     2581 ggtgcggcgg atgtggacaa atgggcgctg tatgtcatcg gccagtactg cgaccagtca
     2641 gtgccggacg gctttggcgg cacggagccg cgcatcacct gtaatgcgta cctgaccaca
     2701 cagcgcaagg cgtgggatgt gctcagtgat ttctgctcgg cgatgcgctg tatgccggta
     2761 tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaggt gtggacctat
     2821 aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc
     2881 ctgaaagacc gccataatac cgttgaggtg aactggattg acccgaacaa cggctgggag
     2941 acggcgacag agcttgtgga ggatacgcag gccattgccc gttacggtcg taacgtcacg
     3001 aagatggatg cttttggctg taccagccgg gggcaggcac accgcgccgg gctgtggctg
     3061 attaaaacag agctgctgga aacgcagacc gtggacttca gcgtgggcgc agaagggctt
     3121 cgccatgtac cgggcgatat cattgaaatc tgcgatgatg actatgcggg gatcagcacc
     3181 ggcgggcgcg tgctggcggt gaacagccag acgcggacac tgacgctcga ccgtgaaatc
     3241 acgctgccag cctccggcac cacgctgata agcctggttg acgggcaggg gagtccggtc
     3301 agcgtggagg ttcagtccgt caccgacggc gtgaaggtaa aagtgagccg ggttcctgac
     3361 ggtgttgctg aatacagcgt gtgggggctt aagctgccga cgctgcgcca gcgcctgttc
     3421 cgctgtgtga gtatccgtga gaacgacgac ggtacgtatg ccatcactgc cgtgcagcat
     3481 gtaccggaaa aagaagccat tgtggataac ggggcgcact ttgacggcga ccagagcgga
     3541 acggtgaatg gtgtcacgcc gccagcggtg cagcacctga ccgcagaagt caccgcagac
     3601 agcggggaat atcaggtgct ggcgcgatgg gacacgccaa aggtggtgaa gggggttagc
     3661 tttatgcttc gcctgaccgt ggcagcggat gacggcagtg agcggctggt cagcacggcc
     3721 cggacgacgg aaaccacata ccgcttcacg caactggcgc tgggtaacta caggctgaca
     3781 gtccgggcgg caaatgcctg gggacagcag ggcgatccgg catcggtatc gttccggatt
     3841 gccgcaccgg cagcgccgtc gaggattgag ctgacgccgg gctattttca gataaccgca
     3901 acgccgcatc ttgccgttta tgatccgacg gtacagtttg agttctggtt ctcggaaaag
     3961 cggattaccg atatcaggca ggttgaaacc agcgcgcgtt atcttggtac ggcgctgtac
     4021 tggatagccg ccagtatcaa tatcaggccg ggccatgatt attattttta tgttcgcagt
     4081 gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag cgatgatgcg
     4141 gaaggctacc tggatttttt caaaggcaag ataaccgaat cccatctcgg caaggagctg
     4201 ctggaaaaag tcgagctgac ggagaataac gccagcaaac tggaggagtt ttcgaaagag
     4261 tggaaggacg ccaacgataa atggaatgcc atgtggggcg tcaaaattga gcagaccaaa
     4321 gacggcaaac attatgtcgc gggtattggc ctcagcatgg aggacacgga ggaaggcaaa
     4381 ctgagccagt ttctggttgc cgccaatcgt attgcgttta ttgatccgtc taatggcaac
     4441 acaagaccaa tgtttgttgg tcagggcgat cagatattca tgaacgacgt gttcctgaag
     4501 cgtctgacgg cccccaccat taccagcggt ggaaatccgc cggcattttc cctgacacca
     4561 gacggaaagc tgaccgctaa aaatgcggat atcagcggta atgtgaatgc aaattcaggg
     4621 acgctcaaca atgtcacgat taatgaaaac tgtcagatta aggggaaact gtcagccaac
     4681 cagattgaag gcgatattgt caaaacggtc agcaagtctt tcccccgcac gaacagttat
     4741 gccagtggca ccatcacggt aagaatcagt gatgatcaga agtttgaccg gcaggtcatg
     4801 ataccgccag tgttattccg cggtggtaag catgagaatt tcaacagtaa taaccaacag
     4861 tcatactggt attcaacctg ccggttaaga gtgacccgca atggtcagga gatttttaat
     4921 cagtccacga cggatgctca gggcgtattt tcctcagtta tagatatgcc tgccggacag
     4981 gggacgctga cactgacatt caccgtatct tcatcaggag cgaataactg gacaccaaca
     5041 accagtatca gcgatctgct ggttgtggtg atgaaaaaat ccacagcagg tatcagtatc
     5101 agctgaattt tataacccag aacgggcgtc agaaatgacg ccttttttat tgcagaaaag
     5161 cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ccgcagccat ttgtctggcc
     5221 gtatccggtg cgcctgcatg ggcgtctgaa catcagtcca cgctgaacgc ggggtatctt
     5281 catgcccgga cgaacgttcc cagcagtgat gatctgaacg gaattaacgt gaaataccgt
     5341 tatgagttta cggatacgtt ggggctggtg acgtcgttca gctatgcagg agacaagaat
     5401 cgccagatta cccgttacag cgatacccgc tggcatgaag attccgttcg taaccgctgg
     5461 ttcagcgtaa tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg
     5521 gcgggtgtgg cttacagccg tgtgtcgact ttctccgggg attatctccg cgtaactgac
     5581 aacaagggga aaacgcacga tgtgctgacc ggaagtgatg acggtcgcca cagcaacacg
     5641 tctctggcgt ggggagctgg cgtgcagttt aacccgaccg aatccgtggc cattgatatt
     5701 gcttatgaag gctccggcag tggcgactgg cgcactgacg gtttcatcgt gggtgtcggt
     5761 tataa
//
LOCUS       DAZFZC010000191         5743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFZC010000191 DAZFZC010000000
VERSION     DAZFZC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.193"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1154)
                     /locus_tag="ACHKXP_004919"
     CDS             complement(<1..1154)
                     /locus_tag="ACHKXP_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7649729.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLAPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHVQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWVCGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRLATAILNAAGRTS"
     gene            1414..1749
                     /locus_tag="ACHKXP_004920"
     CDS             1414..1749
                     /locus_tag="ACHKXP_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO7649730.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNIVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQNP"
     gene            1820..1936
                     /locus_tag="ACHKXP_004921"
                     /pseudo
     CDS             1820..1936
                     /locus_tag="ACHKXP_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2030..2161)
                     /locus_tag="ACHKXP_004922"
     CDS             complement(2030..2161)
                     /locus_tag="ACHKXP_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7649731.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2661..2737)
                     /locus_tag="ACHKXP_004923"
     tRNA            complement(2661..2737)
                     /locus_tag="ACHKXP_004923"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2701..2703),aa:Arg,seq:tct)
     gene            complement(2839..2914)
                     /locus_tag="ACHKXP_004924"
     tRNA            complement(2839..2914)
                     /locus_tag="ACHKXP_004924"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2879..2881),aa:Ile,seq:cat)
     gene            complement(3058..3879)
                     /locus_tag="ACHKXP_004925"
     CDS             complement(3058..3879)
                     /locus_tag="ACHKXP_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7649732.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3894..4250)
                     /locus_tag="ACHKXP_004926"
     CDS             complement(3894..4250)
                     /locus_tag="ACHKXP_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7649733.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(4263..5312)
                     /locus_tag="ACHKXP_004927"
     CDS             complement(4263..5312)
                     /locus_tag="ACHKXP_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7649734.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVHQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRPLYRESDIVPGEQTAISILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPEPPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(5314..5592)
                     /locus_tag="ACHKXP_004928"
     CDS             complement(5314..5592)
                     /locus_tag="ACHKXP_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649735.1"
                     /translation="MAHDTKLHNSDDSAVFASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
BASE COUNT         1493 a         1553 c         1285 g         1412 t
ORIGIN      
        1 gaggtgcgcc cggctgcgtt cagaatagcg gttgccagac gatccggaat aatgctcctg
       61 cgcgcccatg aactgaaatg tgtcgggcgg tttgatgata cctggtttcc attcgttctc
      121 gatgccgcac cgtaatatcc tgatgccgga atatccggat cttctgccgg cgcgttagtg
      181 gcggtattga cgccgttacc gtctgtcatg aagggcacaa aataaacgcc ctcactctcc
      241 ctgtttttat acccgccgta cacggtgtcg tactgggtag cgtatgtatt tttccagtaa
      301 tacgtcgtgt caccacaaac ccacggcaca tctgcagcac tgccaccatg gcactgcgcg
      361 ttaaacacgg agaggtcagc acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc
      421 tgtacgtggg tggcggcgct catgtcaaac tctccctgca tccagcacac cgccagcaac
      481 acatttttcg ggttcttctg taatgcagct ttagtgcgcg caatcaggtc ctgatataac
      541 ggtttaccca caccccagcg tgccgaatcc tggctggccc ccgtgtccgc actgaatgtc
      601 ccctccgcgc cctgggtgaa tgccgaacca ccacgacagc atggtgccag caggatcccc
      661 gcgttattcg ggatatacgg aagcagtttt ttggcaatat gtaagccctg gccgacacag
      721 ccgtactgcc ctttgctcag gtctgccttc ggatgattca gcgtactcat atcctgcaca
      781 tcatgcaggc agtggtcggc cggaataata tcgttatatc tgcaggcagc cccacccggc
      841 gtcactgtac tgcggcgcgc cagctgttta atgcgcggat ccggagcatc gtatgaatcc
      901 ggcagcggaa gcccttcacc gtaagccatg gcattggact gcccggccag tacgatgacg
      961 tagtaccaat ccggctcagt tgcaccactg accaccacat caccttctgc tgtaatcgcc
     1021 tgcatcagag tataaggggt tatggccacc ggactaccaa acggctgcca gccctctttc
     1081 agtttatgtg tcagcttttc cgcaagatct gatggcgacg ccgccctgac aacatcatag
     1141 tgtttaaatg ccatggttct ttccaccatc tgaaaaataa ttctttaaaa tacctgacat
     1201 gtaatacaga aaaaacacaa aaccatacct taaataaaaa cctgattatc aagcagatat
     1261 gcatggataa actacaagac gagatataaa ccaccctgta tttaaataaa caataaacaa
     1321 catcagaaaa ataattctgc tctatggttt aattcaaaaa tatcatttat acttttcaga
     1381 acatcaccag caaggcataa acaaggaaag taaatgaagt ggattgtgat tgatacagtt
     1441 atccagccat catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc
     1501 tggtatcaat cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggt
     1561 atcatgctca ataacaaagt gctacctgta accatttaca acatagtacc attcaataaa
     1621 acattctgga atttaatcaa aaacagccag gaatgcccta caaatacaga taacgtattg
     1681 aatgaatgct ttaataaccg ttgcactctg caaatatgtc cttatgggct aaaacaacaa
     1741 aatccataag gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg
     1801 ggctggagag tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt
     1861 accagcacct gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag
     1921 ggggattcac catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc
     1981 gcctgaattg tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg
     2041 gcgttttctt tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt
     2101 cagccagcac tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca
     2161 ctttttaccg taacaggtga taatccagat atgtatctac cccagatgag taatccgaag
     2221 ttcatccata ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc
     2281 tgaattagca accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat
     2341 aacgtctctt cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc
     2401 ctttgctcgt gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc
     2461 ctcatcaata ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac
     2521 cattagaccg tataacaaat attcaatcaa tacttaacag aacttttatt tttgacaaac
     2581 atataatatt ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa
     2641 ggaatctgga agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt
     2701 agaaggcaat tgctctgtcc ggctgagcta acaacgctga ataccgataa tggatcgcca
     2761 tcggggaccc gccccccgca ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa
     2821 gctaatggcg gtttgtgatg gtggcccttg ctggatttga accagcgacc tggcgattat
     2881 gagtcgctcg ctctcaccac tgagctaaag ggccgggagc agaataataa cgatccgtaa
     2941 ttaatttcgc aataaaaaac ccgctcagcg gcgggttgta gaaactcttc taacgtcagg
     3001 cataaaacgc ccatcgttat gacgaattta ccacagattc cggaaaaatc aaccttgtta
     3061 cctagttacc ttttttaact gccgctcagc ccatgcttct tcaatatcaa accgggtcac
     3121 cagcgcatca taaaatttct taactgtttt ttcccatgac gcgcgtgtta tctggtttgt
     3181 cacctcgcat atagcattaa atgcctccgt tgatggtagt ctttcatagc cacgaccacc
     3241 acaacgctgg cagtctctga taacaggcat accacgtttt accgactctt cacgatgaat
     3301 ggcgacacca cgcccacggc aatccttaca ggcggtggaa acctcaccct ttccgccaca
     3361 ctccggacag gcaactttta ccacctccct gacttttttc cattcttccc agtaagacgg
     3421 atacacacct ttcgtacact ttgcccatac cggcggctta ccatccggat actggacctt
     3481 gtttgtaaaa actatgcttt caataaattt ttccccatag caacaagggc actgcttttt
     3541 actcgctgcg ctgcgggcat aatcctcaaa agcgtacgaa gccataatgc gcatcactac
     3601 cggttttatt tctgccggaa gttttctcaa cgccgccaca cgatcgcacc gactgagtgc
     3661 ataatctgcc agtaattctg ttgcccgcgc cctgtcattc atactgatgc ccattttccc
     3721 caggaacgca gaaaaaccca tctcagcccg attctgtgtc atgccctgcg cggccatcac
     3781 atcagtgata ctcagcgcat cttttgacgt tgaggccgat gcatcggtca ggccagggga
     3841 ttttggggag tagtatttcg gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc
     3901 attatggggt aaatcttcac ccccagacgt ccaccagata ctggctgacc acgaacgata
     3961 ttgatttcat caaactgctc atcgtccatt aacactcccg catgcgtcag cgcatccagc
     4021 ggtgctttca gaatgttgtc caggtcgcga cgacgcttat ccggtggctc tgcaatcacc
     4081 tttatcgcca gccttccgga caggcttaat ttcagccgct gctggcgaac aataagcgcc
     4141 acagcccggc gataacgctt tccctcctcc gagataaaat atgtgctgcc acggcgtcgc
     4201 cagtaagtgt tcaccgtcgg cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc
     4261 ttttacccaa gcacgccagt tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc
     4321 tgagaaccat gcttttcttc gaacgccagc ggatccgcat gaagctcgtt gtgatgctcc
     4381 cgacacagcg gtagcgtgaa aatatcgtga gattttgtcc ccattccgcc ctgaccatga
     4441 ccaatcaggt gatggggatc gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc
     4501 acccagcgtg tgtatttctc gttaacccag cggcgacgtt taggtcgttt catgaaagat
     4561 tccggaggct caggatcaac ggcaatgctg accaccgtct tttcctgtgg tgggttctgt
     4621 tgctggtggg cgtgaggcag cggcgcaaga ttttttgtgc gctgtttcag tatgctgatg
     4681 gcggtctgct ctcccggtac gatgtcgctt tcgcggtaca aggggcggat tttttccgca
     4741 cgtaacccca gagaacgacg taatactgcc tccggaagcg cgtccgccac ctgattgcag
     4801 accgcccacc aggataattc agccagagat aattcacgct cctgcgtacc gcttattgcg
     4861 tgaccgatga cgtcaatcat ccatgctgac aggttttgat gagcaagttg ctcgagtgat
     4921 tcggatgtct ggtcacgcag ctggttgtcg cagtgccagc acaacaccat tgcgccggta
     4981 ccataacggt gaatgacggt ttcgctgtga tgataatcgc cgtgtggcca ctggcaggat
     5041 ttaatatggc gcaacagcca gtcagacaat gcaccagcac caccagcagc acgaatcacc
     5101 cgtgcgttac tgaaaaacgg cagcaatgtt ttgtcttcca ctagcggctg gtgaacggca
     5161 ggaacaaccc cggacggcag attacgcatg cttttcggtt ccggctccac cagtacccgg
     5221 gtattgtgga ataccggcat ggattcacgg cccggcttaa cgatcaccag cccgagttcc
     5281 ggtaccagaa caggtcgaag taatacccgc acgttacctc cagatgcgtt gctggaatgt
     5341 gcgggacgga cgcggtgggc gctcggagta aggaagcctg acggagatta tccagtgacg
     5401 ataatcgagg ctgagggctt tcttaatctc gtatccgtgt ctgcggtagc actgaattag
     5461 ccactcggcc tgttcttcag tgcatggggg atgctggaac cagtcagatt tgaaagtgcg
     5521 ggaacgccgc ccgtgcctgc tggcaaagac ggcagaatca tcagaattgt gtaatttggt
     5581 atcgtgcgcc atcggttgtc tctgctggcg cagcaggtgc cagttgttca ggctggcgtg
     5641 cgaattgtaa accagaatgc taggaaaaaa caaaacccgc cgaagcgggt taagtgcggg
     5701 tgcgttgagg atgcctgaca catcagaggt ggcgagggat tct
//
LOCUS       DAZFZC010000192         5697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFZC010000192 DAZFZC010000000
VERSION     DAZFZC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.194"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(168..1181)
                     /locus_tag="ACHKXP_004929"
     CDS             complement(168..1181)
                     /locus_tag="ACHKXP_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO7649736.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGSLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(1193..2509)
                     /gene="fucP"
                     /locus_tag="ACHKXP_004930"
     CDS             complement(1193..2509)
                     /gene="fucP"
                     /locus_tag="ACHKXP_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7649737.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(2537..3457)
                     /gene="rbsK"
                     /locus_tag="ACHKXP_004931"
     CDS             complement(2537..3457)
                     /gene="rbsK"
                     /locus_tag="ACHKXP_004931"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7649738.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            3763..4545
                     /locus_tag="ACHKXP_004932"
     CDS             3763..4545
                     /locus_tag="ACHKXP_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO7649739.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(4547..4773)
                     /locus_tag="ACHKXP_004933"
                     /pseudo
     CDS             complement(4547..4773)
                     /locus_tag="ACHKXP_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(5253..5423)
                     /locus_tag="ACHKXP_004934"
                     /pseudo
     CDS             complement(5253..5423)
                     /locus_tag="ACHKXP_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1641 a         1354 c         1185 g         1517 t
ORIGIN      
        1 aatactagtt tttagactat cacttattta agtgatactg gttgtctgga gattcagggg
       61 gccagtctaa tcgtctataa gggcaacgct atcatagtct tgtcctggcg ggtaaaaaaa
      121 cgcgcttacc ttaacgataa gcgcgccgct gttcaggcct taagtggtta ttcaattcct
      181 gtggtgactg taaaagtgcg cgtttgctgc ggtgcaacct gaatcagcga gccattacgt
      241 tgcgcggcaa gatacccctc aggccgacag gttgcaggta atgcaaaggc ggctacctgt
      301 tgctctccat tataaaggat ccagcgtgtc acataattta gttcagcact ggagaaacga
      361 gtaacaaacg tggtgccatc gggagagatc atgcgaaact ctggctgatc tgtgtaagca
      421 tccagtttgt ctgcaaagaa gactatttct ggatcataaa attccggttg attcagcgtc
      481 gacagagagg cttctccctg cataatccgt tgattaaacg ccagccactg agcggtggga
      541 ttaacatgcg aaggtactga ttcacgcaac cttaatattt cgtccggtat attctggctg
      601 aacgtagcat ttggtatata tgcataattc atgtggcaca tatattgtag tggcatatct
      661 acagaagcca gattggttac ggccatctta atatcgaaca atgtagagga tttgtgaagg
      721 accactgttg gctgagccag ataatgatgc ccgaacccca ttacatactc gtaacgcccg
      781 gtaaggcgta acatatctcc ctctaattcc atccatgctt catccatcgc ggcacaggcc
      841 atttcaccgt gtagcagatg agtatcttcc gcagatgggc agccattagc cagcaaacct
      901 gaatgaaagg caaaacagcc ataggtctct atcacctctg tcgccggttt aggctggcga
      961 aacatattgc acatggtgag actgtgtcca tcaaattgcg catcccaaat catctgcccc
     1021 atccagggaa gaataattaa atgtccacga ctgtttgcaa ttttaagccc ctcgacacca
     1081 ctgtcatagc gaaaagacgt gacagtaaaa tcactatttt ccagcaagat acgaggtttt
     1141 tcgccaaaaa gcgcccgcca caaattaata cgcgtactca taacggttct cctcaggacg
     1201 ctgtgacttc agccagtgcg gtacgtactt tgctttcacg ccagaagtag actccgacat
     1261 agacaaagca gagcatagaa accaggaatg aaagctgaag tgagtggaac atatctgcaa
     1321 tatatccctg aattgccgga accaccgcgg cgccgacaat agccataaca atgactgctc
     1381 ctgccatttc tgtatgttcg ttatcaactg tatccagtgt tcctgcatag atcgtcgccc
     1441 agcaagggcc aaacaaaaca cttaccagga cggcgacata gaccgcgctg aaacttggag
     1501 ccagtgcaac atatgccagg aacagcgccc ctataacgga atagagaatt aatacttttt
     1561 ccggattaaa acgcgtcata aggatgttgg ctataaactt gccaataaag aagcaggcaa
     1621 agctatagac catgaagttt gaagcatcac gttcgttgat atcgcccaac tccagtgcca
     1681 gacggatggt aaatgaccat actgcgacct gcatacccac ataaaggaat tgagccacaa
     1741 taccacgacg aaagcgcgaa tttttagcca gatagcgcag cgtatccatt gctgacgggc
     1801 gtttatggtg acttgtctgt gccactttac aggttgggaa gcgggttaaa aggaacaaca
     1861 ccatgaccac aaccagaatc ataatcatat acttatacgg ttcaagggtg ttctctaaca
     1921 tcagcacctt aaagttgtga atttgctcgg cgttcattcc ggacatctgc ttctcaaggc
     1981 tttccccctc ggagaaaacc agatatttgc ccaataaaat accagacgca gcaccaatcg
     2041 gataaaaggt ctggctgata ttgagccgca atgtggcata ggcttttgga ccgatcattg
     2101 aactgtatgt gttcgctgca gtttcaagga aactcaggcc aatcgcaatc gcaaaaatag
     2161 ctgcaaggaa catagtgtag gttgccatat gcgaggcagg gaaaaaaagt gtacaaccac
     2221 caatatacag cgtcaggcca attaaaattg ccaccttata actggtcttt ttaatcacaa
     2281 gggatgctgg tattgcaatt aaaaaataac ctccataaaa tgcgctctgc accaatgctg
     2341 aagcaaagtt acttagcgaa aatacacttt tgaattgagt gattaatatg tcatttaatg
     2401 cagctgcgca tccccatagc gggaataaac acgataacaa aataaactgg aacaagggag
     2461 tcttattcag atatccatca ggcatctgaa tgatgttttt atcgttcata gtgctacctt
     2521 taactgtgca ggatgattat tcgtttaagg ttaaaaattc attaaattgt tcaatactcg
     2581 gataagatga ttgcgtacct ttccctgtga cgctgaaagc ggcaaagaga gcggcttttt
     2641 tcaaagcggc ttcaacatca ccgctttgaa cataataatg ggaaaagcaa ccaataaatg
     2701 cgtcaccagc gccactagta tcaacagcat ttactttgaa tgcaggaaca tggacttcct
     2761 gatcgcgggt catccataat gcgccttttt cgctcatggt aacaataata ttgttcagcc
     2821 ctttatcaac taacgaacgt gcggccaaac gaatatgatc ataagtatca accgacatac
     2881 cggttaatat ttccagttct gtttcattcg ggataaagaa atcacatttg caggcataag
     2941 acatatctaa ctcacgcaat gccggagccg gatttaataa cacttcaata ccatttttct
     3001 taccaaactc aatcgcgtgg taaactgttt ccagttgaac ttccagttgt aaaacgatca
     3061 atttgcattt tttcagatct tctgcagctc gatcgatatc ttccggggaa agaaatttat
     3121 tcgctccctt aattattaat atactattgc tcgagttggc attaacaaag atcggtgcaa
     3181 caccactgct ggtacagggg accttctcaa cataagtggt attaattccc catgattcaa
     3241 gattacgaat agtattatcc gcaaaaatat catcacctac tttagtcagc atcaggactt
     3301 ttgaattcaa tttagccgcc gccaccgctt gattagcacc tttcccacca catccgattt
     3361 tgaaggcagg tgcttccaga gtttctcctt ctttaggcat ctgattagtg taagtaatga
     3421 gatccaccat attggaacca ataactgcaa tgtccatttc actacctctt ataaactttc
     3481 gcataacaat ggtatttaaa taacattatc atgttacttt tgcatcattt gtgactgaga
     3541 tcgcgattag cacatcaacc cgatgtttat ttaatagact tccagtctca tcactcaggc
     3601 caacactatc taatcataag caacctaaca agattagtgc ccaaaactca gcagcctata
     3661 cccttttcat ttcaaagggg cggtcgtata gtatggtaat gaaaacaatg tttactaacg
     3721 ccaaaatgtt atttttataa cattcttacg gagagagagt tgatggaaac gaagcaaaaa
     3781 gagcgtatcc gacgtttgat ggaactgctt aagaaaaccg acagaatcca tttgaaagac
     3841 gcagcgcgaa tgctggaagt ttctgtaatg actattcgtc gcgatctcca tcaggaagat
     3901 gaacctctgc cactgaccct actgggtggc tatattgtaa tggtgaataa acccgcgcca
     3961 tccatgccag taatccatga cgttccaaaa aatcatcgtg atgacttacc tattgcaatt
     4021 ctggctgccg gaatggttaa tgaaaatgat ctgatcttct ttgataatgg ccaggagata
     4081 ccactcgtta taagcatgat cccggatgca atcaccttca ccggcatctg ttactcacat
     4141 cgcgtctttg ttgcgttgaa tgaaaagcct aatgtaacag caatactttg tggtggtacg
     4201 tatcgtgcca gaagtgatgc tttttacgat gccagtaact cttcgccatt agactctctc
     4261 aatccgcgaa aaatatttat ttccgccagc ggtgtgcata atcactttgg cgtcagctgg
     4321 tttaaccctg aagatcttgc cactaagcgt aaagcgatga accgtggact acggaaaatt
     4381 ttgctcgccc gccacgcgtt gttcgatgaa gtggcctctg ccagcctcgc accgatctct
     4441 gcatttgacg ttctgattag cgatcgtccg ttaccggcag attatgttac gcactgccag
     4501 aatggttctg taaagatcat tacacctgat tcagaagacg aatgacttac tgaaaaaaca
     4561 ccacaatctt gttaaacatc gtcgggttgg actgattacg ttgcactttc accacatatt
     4621 ccagcttatc tatttggctt atcacctact ccagacgctg gtcatccttg accagtagcc
     4681 agatatggct tttgtcgcag tcctgaatcg acaggcagag aatgtcttca acgttaagag
     4741 cgcgacgggc aaaagcccac aaaggtgagt cattacaccc ggatggtaaa caggcacttc
     4801 cttgttgtag aagccgttat attgtccata atgtctgggt ttttaatgca tactgggtaa
     4861 caaccggacg aatcttcgaa tatcactggc gctgccagcc ggtttgaatt catctcacaa
     4921 ccctgcatag agcgaatctc ctgcctgcac gtcactccac tccatggtat caacttcaca
     4981 ctctttatct gcggctagct tcagccacca gataagcatc gactatgaaa gatgagccat
     5041 gacaacataa cgttggtaac gctctgacgc cttaatggaa gatgcctgcc accataggga
     5101 atgtaaacga ctgaagtgtg gcctttaatg ccgtgaacgg ctcatggtct cctggcacgg
     5161 ttgccgcccc aacctgtaac aacattccac agtacaatgt ctgtcagagt cagagcctcc
     5221 catgcttgtt gtagtaactc taccagttac gaacttcctt cctcgtattg cttacgccag
     5281 agaaataact ggctggctgc tacaccatgt tgccgggcaa cgagggagac cgtcatcccc
     5341 ggttcaaagc tctgctgaac aattgcgatc ttttcctgtg tggtacgccg tctgcgtttc
     5401 tccggcccta agacatcaat catctgttct ccaatgacta gtctaaaaac tagtattaag
     5461 actatcactt atttaagtga tactggttgt ctggagattc agggggccag tctagtcagt
     5521 gatctacagc ctgttgaata aaaccccgga tgcttccccc gattctgcaa gtaatacttc
     5581 ggttatttgc actattttat ccctgttgaa tttggttaag ccggtgcaga cgcgccggga
     5641 aacgcacacc tgaaccggca caataaaata caatacaacc ccctgttact ggagaaa
//
LOCUS       DAZFZC010000193         5677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFZC010000193 DAZFZC010000000
VERSION     DAZFZC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.195"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(87..389)
                     /locus_tag="ACHKXP_004935"
     CDS             complement(87..389)
                     /locus_tag="ACHKXP_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepZ"
                     /protein_id="HFO7649740.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMTQENGVNRNIRI
                     VAGIALATTALAALGTGIAMACTTNASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
     gene            573..926
                     /gene="cesL"
                     /locus_tag="ACHKXP_004936"
     CDS             573..926
                     /gene="cesL"
                     /locus_tag="ACHKXP_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO7649741.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTF
                     QQHVQIIERVRRMTS"
     gene            923..2950
                     /gene="escV"
                     /locus_tag="ACHKXP_004937"
     CDS             923..2950
                     /gene="escV"
                     /locus_tag="ACHKXP_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO7649742.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     IKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            2934..4274
                     /gene="escN"
                     /locus_tag="ACHKXP_004938"
     CDS             2934..4274
                     /gene="escN"
                     /locus_tag="ACHKXP_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO7649743.1"
                     /translation="MISEHDSVLERYPRIQRVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            4334..4654
                     /locus_tag="ACHKXP_004939"
     CDS             4334..4654
                     /locus_tag="ACHKXP_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO7649744.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            4647..5063
                     /locus_tag="ACHKXP_004940"
     CDS             4647..5063
                     /locus_tag="ACHKXP_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO7649745.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNGTIYLSITLNP
                     NNKNHIFLYHKKNYICDKLKEIFPDEPIEFSIEYEN"
     gene            5026..>5677
                     /locus_tag="ACHKXP_004941"
     CDS             5026..>5677
                     /locus_tag="ACHKXP_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO7649746.1"
                     /translation="MNLLNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREV
                     SGNGIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHS
                     NTIFFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNN"
BASE COUNT         1632 a         1020 c         1237 g         1788 t
ORIGIN      
        1 catatttacg gttagtctct tttcatgaga gaggtaatgg cataatgcat ccatgacctc
       61 tctattttat ttatgcgatg cgatgattag gcatatttca tcgctaatcc gccgccaagc
      121 gcagtaacac cacctaatac accggtagca atacctaaac ctaagaattc ttgggagctt
      181 gcatttgttg tgcatgccat tgcgatacca gtacctaaag cagccagagc tgtagtagcg
      241 agtgcaattc cggctacaat tctgatatta cgatttaccc cattttcttg tgtcattact
      301 cctgtatttt catccaccga aacatttcca ttgattgtgg ctgccagtgg taatactccg
      361 ccagaaggac ttaaatttgc tgcatccatt aacttttctc cattgtctaa tttagacatt
      421 tgtctggtta tcaataacca aattatccca tgggcaacta ttatattatt taaatagttg
      481 caatgtacat aaagtacata tactgtatat attatccaat acgctttttc aagcaataat
      541 atccacacat atacttactt cagagcctgc atatgaatct tttaattaaa agaaacgtag
      601 aagagttttt aagattatta ggaaatgatt tttatttgtt tgataatcgt gtggaaattg
      661 attttaatgg tttttctttt tttattgaaa taattgatag taatgtgttt attacgtttg
      721 ccttagagta taacgaaaat gcgttttttt cttttttcaa tgcccttgcc cctgaacgga
      781 cgcagggagt gattgaacat atttttgtct acgataacaa attgtgctta agttgtttgt
      841 taaccaatat tgatgtgttt tctctaatga atacttttca gcagcatgtg cagattattg
      901 aacgagttcg caggatgaca tcatgaataa actcttaaat atatttaaaa aagcagagtc
      961 atatcacgat cttattctgg ctctcttctt ctttatggct gtaatgatga tgattattcc
     1021 attgccaaca gttgtggtgg atatcattat cgcgattaat atatcgacag cgttgctttt
     1081 attaatgctc tcaatctata taaaaaatcc tcttgagcta acttcttttc ctacaatcct
     1141 gttgattacg acgttgatgc gcctgtcgct cagtgttagt acaaccagac ttattctgct
     1201 gcatcacgat gcgggtgaca ttatttattc cttcggtaac tttgttgttg gcggtaatat
     1261 tgttgttggc ctggttattt ttaccattat caccattgta caatttatgg ttattacaaa
     1321 aggggctgag cgtgttgctg aagtgagtgc tcgtttttcc cttgatggta tgccaggaaa
     1381 acaaatgagt attgatggtg atatgcgcgc aggcgttatt gacccgcttg aggcaaaagt
     1441 tcttcgctcc cgagtgcaaa aggaaagcca gttttatggt tcaatggacg gggcgatgaa
     1501 gtttgtaaaa ggggacgcta ttgccggtat catcattgtt ttagttaacc tgtttggtgg
     1561 cgtgctcatt ggaatgtggc aatttgacat gccatttagt gaagcactta gcctgttttc
     1621 tgtattgtct gtcggtgatg ctttggttgc ccagatccct gcgcttatta tttccgtcac
     1681 tgcgggggtt gttgttactc gtgttcccgg tgaaagcgaa aaagaagaaa accttgcagg
     1741 tgatattgtt caacaggttt ctgttaatag tcgccctttt ttaatcagtg ccgcactcat
     1801 gatcgtaatg gcgattattc ctggctttcc tacattggtc ttcttgtttc tggcggtttg
     1861 cctgttgggt atcgcctgga aattacagaa gaaaagaaca tttggaactg gcaataataa
     1921 ggacgctatg ggagctgatt tgtctaatag ccaaaatatc tcaccgggtg ccgagccgtt
     1981 gattttaaac ttaagtagta atatttatag ctcagatatt acacagcaga ttgaggttat
     2041 gcgttggaat ttctttgagg aaagtggaat tccattgcct aagattattg ttaatccaat
     2101 taaaaataat gatagcgcta tagaattttt gctctatcaa gagtcaatat acaaagatac
     2161 tcttacagat gatactgttt attttgaggc tgggcatgca gagatatcat ttgaatttgt
     2221 ccaggaaaag ctttctgcta actctatcgt gtataaaacg aatgaggcta accaacaact
     2281 agctcacgtc tcaggtatgg atgtttatgc aaaaacaaat gataagataa cgtttttgct
     2341 aaagaaactt gtattgtcta atgccaaaga gttcatcggc gtacaagaaa cgcgttatct
     2401 gatggacatc atggagagga aatataacga acttgtgaaa gagcttcagc gtcagcttgg
     2461 tctgagcaag attgtggata ttcttcagcg cctcgtagag gaaaatatct caattagaga
     2521 tctacgaact atttttgaaa cgcttatttt ttggtccaca aaagaaaaag atgtggttat
     2581 cttgtgcgaa tacgttcgta tcgccctccg tcggcatatt ttaggtcgct atagcgttcg
     2641 cggtacactt ctgaatgtct ggcttattgg ctctgatatt gaaaatgagc tgcgagagtc
     2701 gatcagacaa acgtcatcag gttcgtattt gaatatctca ccggagcgaa gtgagcagat
     2761 tattggcttc ttaaaaaata ttgtgaatcc aacggggaat ggcgtcattc tgaccgcttt
     2821 agatatcagg cgctatgtga agaaaatgat tgaaggttcg ttcccgtcag tccccgtgct
     2881 ctcttttcag gaagttggga ataatatcga acttaaagta ttaggaacgg taaatgattt
     2941 cagagcatga ttctgtattg gaaagatacc cacgtatcca gagagtgctc aatagcactg
     3001 tgccgacatt atcattaaat tcgtctacca gatatgaagg caagattacc aatatcggcg
     3061 ggacgattat caaggcgcgt ttaccaaaag cgcgtattgg cgccttttac aagatagaac
     3121 ccagtcaacg tttagctgag gtgatcgcta ttgatgaaga tgaggttttc ctgctgcctt
     3181 ttgagcatat ttccggcatg tattgtggcc agtggctaag ttatcagggg gaagagttca
     3241 agattcgtgt tggagacgaa ctccttgggc gactggttga tggcataggc agaccaatgg
     3301 ggagtaatat tactgcacct tatcttccgt ttgaacgcag tttgtatgct gaaccccccg
     3361 atcctttatt aaggcaggtt attgatcagc catttacgct tggcgtcagg gcaattgatg
     3421 gtttacttac ttgcggtatt gggcaacgca tcggtatttt cgcggggtcc ggcgttggta
     3481 aaagtacgct tttgggaatg atttgtaatg gcgcatccgc agatattatt gtccttgccc
     3541 ttatcggtga acgtggtcgc gaagtgaatg aattccttgc gctcttacct caatccacgc
     3601 tttctaaatg tgtgctggtc gtcacaacat cagaccgtcc cgcgctggaa agaatgaagg
     3661 ccgcatttac ggcgacaacc attgcagagt tcttccggga tcagggcaaa aatgtattat
     3721 taatgatgga ttccgtaact cgttatgccc gagccgcgcg tgatgttggg cttgcatcgg
     3781 gagagcctga tgtaaggggg ggattccctc cgagtgtttt ttcctcgtta cccaaattat
     3841 tagagcgtgc ggggcctgcg ccaaaaggct caataaccgc gatttatacg gtgttgttag
     3901 aaagcgataa tgttaatgat cccattggtg atgaagtccg ctctattctt gatgggcaca
     3961 ttgtgcttac ccgagaactt gcagaggaaa atcattttcc tgcaattgat attggtttaa
     4021 gcgccagtcg tgttatgcat aatgttgtta catcggagca tttgcgtgcg gcggcagaat
     4081 gcaaaaagct tatcgcaacc tataaaaata ttgagctgct tattcgtatt ggtgagtaca
     4141 cgatggggca agatcctgaa gcggataagg caataaaaaa caggaaagca attcagagct
     4201 ttatacaaca aagcaccaaa gatatcagta gttacgaaaa aacgattgaa agcctattca
     4261 aagtggttgc ctgaatatgt tggacagaat tttatctatt cgtaaaagca gagcgaatcg
     4321 attgagagaa tcaatggcta agattaactc tcagataaaa gaggtggatg gtaaactcga
     4381 tgactgtgag caagccatta aagagtcgat tgcctctaag caggcgtatt gtgcatcgct
     4441 ggtgaatctg gataaagtgt cgttgtataa ataccagatt aaaaacaatg catttgatga
     4501 gcaaaagcag cgattatatg agaaaaaatc ctcactttct aaagaaaagc gttcattgct
     4561 cgattcacaa aaacgaacaa aagaggatct acaacatgtt aataaatcaa tagagaagtt
     4621 atcttttgcc ataaaggaac attactttga ctagcatttc tctaaaaaga aatttgatag
     4681 agcctgtttc ttttcagccg tcagatgatt ggccgcaggg agaagaaagt tttaatgata
     4741 tttttaatcg cataaaaaaa agaacacctg acacttcgtt catacgaatg gcttctttat
     4801 ggaaacgatt gttctacgct cctggacaga ggcgacgcta ctttgaacaa ggagagcgtt
     4861 catttagtat tatttgtgga aagttacgcg gaattgtttt aacaataaaa tgtagtaatg
     4921 gaaccattta tctgtctatt acactgaacc ccaataacaa aaatcatatt tttttatacc
     4981 ataaaaagaa ttatatttgt gataagttaa aagaaatctt tcctgatgaa cctattgaat
     5041 tctcaattga atatgaaaat taatgatttt tacttgccat tgttgtcagt cataggaacc
     5101 ggtcgcctct atattacgcc tgaagggcat gcttgccatg catattttcg cgaagtcagc
     5161 ggtaatggta tccgcttcac gttaacatgc tcaggctatg aggggcgttt ttggatatca
     5221 gaagaacagt tcattcagtg gtgccaggag ttattcccgt atagcgaaag ccggctgata
     5281 ccggaagata tgataaaact catgatctta tgggtaatgc agactgcttt accggaaggt
     5341 gatgtgtctg ttgatgatgt tcaatttacg atgctaaata aagatgttta tccggtaata
     5401 gaaaataata atggagaaaa cagacttaat gtaattatac tcgaaataac ggtacagagt
     5461 ttacaatatc taattaatga aaattggcaa tttgttccac attctaatac cattttcttt
     5521 gatgggtata ttgctcctgg gtggactgat tacccggtta cagagctgac tgttggtgat
     5581 agtttaaggc tctatcatgt tgatgacagc aaagaaagag agtgttggct tgttattaat
     5641 aaccctttag caaccgtaaa actttgtgat aataatt
//
LOCUS       DAZFZC010000194         5545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFZC010000194 DAZFZC010000000
VERSION     DAZFZC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.196"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..313)
                     /locus_tag="ACHKXP_004942"
     CDS             complement(<1..313)
                     /locus_tag="ACHKXP_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649747.1"
                     /translation="MTKSWSVPFPESETEHEGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVDFKPAPNQWLQEWKQRINRHAIKKVAKNAERSFAFPTKKLAI
                     ES"
     gene            complement(514..819)
                     /locus_tag="ACHKXP_004943"
     CDS             complement(514..819)
                     /locus_tag="ACHKXP_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649748.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLLCKKADEVERKQNRIYYRKPRCEMGVTCSGRQKQRGKSIPAYYD"
     gene            complement(1253..1549)
                     /locus_tag="ACHKXP_004944"
     CDS             complement(1253..1549)
                     /locus_tag="ACHKXP_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649749.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHLID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
     gene            complement(1590..2297)
                     /locus_tag="ACHKXP_004945"
     CDS             complement(1590..2297)
                     /locus_tag="ACHKXP_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7649750.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRESSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            2409..2633
                     /locus_tag="ACHKXP_004946"
     CDS             2409..2633
                     /locus_tag="ACHKXP_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7649751.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            2743..3036
                     /locus_tag="ACHKXP_004947"
     CDS             2743..3036
                     /locus_tag="ACHKXP_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO7649752.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            3069..3215
                     /locus_tag="ACHKXP_004948"
     CDS             3069..3215
                     /locus_tag="ACHKXP_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFO7649753.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            3208..4068
                     /locus_tag="ACHKXP_004949"
     CDS             3208..4068
                     /locus_tag="ACHKXP_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7649754.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKTVTALSPKQGHTKETITKEKRNNK
                     NTMSESVRTECEKSPDRHEETDKAFEEIFWCAGMRKAGKKNAASAFRTQFREWRKTTR
                     GTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEKPETIQPQSKPSSAITV
                     SKTGYVFFDR"
     gene            4176..>5545
                     /locus_tag="ACHKXP_004950"
     CDS             4176..>5545
                     /locus_tag="ACHKXP_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO7649755.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFADGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYG"
BASE COUNT         1505 a         1233 c         1315 g         1492 t
ORIGIN      
        1 aactttcaat ggcaagtttc ttcgtcggga atgcaaaaga tctttctgca ttttttgcta
       61 ctttcttaat tgcatgtcta tttatccttt gtttccattc ctgtaaccac tgatttggtg
      121 ctggtttaaa atcaacaatc caatgcgcag gaaccaacca tgcataatgc tctgtctgat
      181 gaaaagctat atattgaagt gcgaatattt tgatcccatc ttcttcaact gtcgcctgga
      241 atctccagaa aacaggcatt ccctcatgtt cagtttctga ttcaggaaaa ggtacgctcc
      301 atgattttgt catatatcac ctcaaataag tggtttgctg cgaaagtaaa tacgcttaag
      361 ttacctgtta tttatcccac caagttccgt atctatctat ccagttacac caatcatcga
      421 cactccattt tgttgtgtcg cattttggca actggcatga atatctacct tctttgtaaa
      481 gtcggcgttt gactttcttg agcatggctc acctcaatcg taataagctg gaattgattt
      541 tccgcgttgc ttctggcggc ctgagcaagt cacacccatt tcacagcgtg gcttgcggta
      601 gtaaatacgg ttctgtttac gctcgacttc atctgccttc ttgcagagaa ggcttccgag
      661 tgatgctgct ttgtctgctc tgacgcaacc agagagcttt agcgcaattt ttcgcgccag
      721 tcgctgttct tgcattgcct gctcacgttg agcctgtctg cgtgctctgc ggcgatttct
      781 ggcgttatcg tcagccagat atgtaatgac tactgtcatg ttgacctccg atgattgact
      841 ttggcggtga cgcgccgggt gcttatcttc cggttgccgt cgtgcagctg cacttcacgt
      901 caccccaaag ccaactactc tttggttccc gcatttcggc gggacaatcc catcaatgtt
      961 aaagagcctg ccaatctgtt ccgtttggct tccagcgccc tgctgatggc ttaaagataa
     1021 cttaggttat aggcgtggtc aataacctaa tttatatttt atggtaaata agttataagt
     1081 gatggataac aaaggtattt tatttttgta aatgttgctg attgattggt gtttgagggc
     1141 ttgcgtgcgg ggtgaaggtg ttacctttgg cttgatgctt gtctatgatg aggatggttg
     1201 attgggtggt aaacggcagg aaaagaaaac ccggcgctga ggccgggtgt ttttagtctt
     1261 ttcttttgct tagcatttcg tcgatttcta agtcaatacg atattgatct attgcttttc
     1321 tctcgtttgg ggttggtatt ttatactgtt caattagatc tgttgtgtat tttatttcat
     1381 ctaatgtgat ttttatatca gagagaatct cttttatatc aattagatgc tcttcttctt
     1441 ttgctcgatt agagctatgt tcaatcattt gagatagctt tttgctaatg cttagcaaaa
     1501 caaaaagaat gatgaccaaa acaacaacaa aaactatcag aaattccatt atccctccgc
     1561 acttccgtaa gtcttcttct gattatcggt taccaactat gagacgacca gaatactctg
     1621 ccaataatcc ttacggtttc atgaaattca tctctatcca ttacttcatc cgggtactct
     1681 tcgcgattta ttgatctgat tatcaccgat gtaggggtgg cgattaatgt ttttactcgt
     1741 aacaaatcag actggcaaat agcgtaggtt ttaccatctc tgattgtggt atcttgcgtg
     1801 ttaacaccaa caacatcgcc atcgtgaagc gttggttcca tgctttgccc tacaacccta
     1861 actagcttgg ctgatctttc agatactccc atctttttca gatagtgctt tctgaaaacc
     1921 aaagagaact ccgatgattc ctctagctcg cagctaccgc ttccagctga aagcgaaacg
     1981 ttaagaagag gcaacgcaac aaactcgtca tcgtttcttt taatgtcttc ccataccaca
     2041 gcttttaaag atgactcacg gacattggat ggttcttcat gtgcaccatc cctcatttca
     2101 ccaataccag aactaagcca ttcagggcgc acttttaaag cattggctaa ttcaaccatc
     2161 ttgcgagatc cgtttgtttt accggacgac atcttctgta tggctggctg agatattcca
     2221 accatgtcag caagctgtga ttgtgatacc ccagctgagc tcatggctgc atttagtctt
     2281 tctgcgaatg ttttcatacc cacaaatcta taactacggt tatccaaagt aaaataacaa
     2341 aggttattgc tattttttat aacttgagtt atctttggtt ataatcaatg accacaagag
     2401 gtatgctcat gaatttagta attcaacgag ccttgaaaat tgtcggtagc caaaagcgcc
     2461 ttgccgacaa gtgtggtgta acgcagccag cagtacacaa atggctgaaa ggcggattgg
     2521 tctctccaga gaaagttacc gccatcgtta acgccactgg agggcagatc aaggcttacg
     2581 aaattcgccc cgatttgcca cacctgtttc caaaaccgaa tcaggcagca taagtaacac
     2641 cgctctttaa cagtcatggt cctcattccc gccgaaatgc gggaatacaa cgcgcataag
     2701 ttgatgcgca taacttctta tttgttaagg aaatacttac atatggttcg tgcaaacaaa
     2761 cgcaacgagg ctctacgaat cgagagtgcg ttgcttaaca aaatcgcaat gcttggaact
     2821 gagaagacag cggaagctgt gggagttgat aagtcgcaga tcagcaggtg gaagagggat
     2881 tggattccaa agttctcaat gctgcttgct gttcttgaat ggggcgtcgt tgacgacgat
     2941 atggctcgat tggcacgaca agttgcttcg attctcacca ataaaaaacg cccggcggca
     3001 accgagcgtt ctgaacaaat ccagatggaa ttctgaggtc attactggat caatccacag
     3061 gagtaattat gacaaaacaa ctcagtcctt accaggacaa aattcacaaa cacatactac
     3121 gtgatcgctt cctgtccagc ttcaagcagc ctggtcgatt ccgggctgag ttggaaaaag
     3181 tgaagctgat gcagaaggag aaaggtcatg agtaatcttg caaccgtaac acatttaagg
     3241 ccttcacaac ggcctgtgga gcgtcgtgtg gcagaagttg aagatggtta tacccgtctt
     3301 gcaaatgccc tgtatgaaga gcttatcggc gcagatttaa cgaaaaatca gagcaaggtt
     3361 gcccacgcca tatgccgtaa aacatacggc tacggtaaaa agatggatcg catctctgat
     3421 agtcagttag ctcaaattac caggctgcca agacagaagg taaacaaggc caagaatgag
     3481 cttatcgcga tgaaggttat ccttcgcgaa ggccagcaaa tcgggcctaa caagaacatc
     3541 gaggaatggc aaatcgaagg gtgtcactac tctggtgata atgtcactgc attggtgaca
     3601 aaaagtgtca ccaaaacggt tacagcgctg tcaccaaaac agggacacac aaaagaaact
     3661 attacaaaag aaaaaagaaa taataaaaac actatgtccg aaagtgttcg gacggagtgt
     3721 gaaaaatcac ctgaccgtca cgaagaaacc gacaaggcat tcgaggaaat attctggtgt
     3781 gcaggcatgc ggaaggccgg gaagaaaaac gcagcttcgg cattcagaac acagttcagg
     3841 gaatggcgta aaactaccag gggtacggca agcgagtttg ccacgatgct ggcagaagac
     3901 atcgcatgca ggaatggtaa gcagttcgga ttcgacaggt tgttaccatc gagctacctg
     3961 aacggtcaac gctggaacga cgagaagcca gaaaccattc aaccacaatc caaaccatca
     4021 tccgcaatca ccgtatcgaa aactggctac gtgtttttcg acaggtgaac catgaaatca
     4081 aaaatcaaat cgctactggt cgctggttat aaccacggct ggttaagtat ttcgtttgtc
     4141 gatttctggt ttaaaaatct caatctgagg gaatcatgag gccaagtgaa cttagcgacc
     4201 tgctttgggc gcaggttgac agggtggctc cgcacctgtt gccaaacggc aagaaagagg
     4261 ggcatgagtg ggttgccggt aacgtcaacg gtgacaaggg aaacagcctt aaggtcaacc
     4321 ttagcggcaa gaaaaaatgg gctgatttcg ctgatgggga cggcggtgac atgcttgatt
     4381 tgtggatggc atgtcgagga attaacctgc atcaggctat gcaggaagcg aaagcatttc
     4441 tcggtatcaa ggatgacgat caccatttcg atgccaaacg tgagaagaaa ttctccagac
     4501 ctgatcgcaa gaaaatcgcc cgctacgtta ccagaacaga atcccatctt gagtacctgc
     4561 aatcgcgtgg catatcgcca gaagtcgtaa agcgctacga ggttgtcagc ggaaaggtgt
     4621 ggaatggaga acgagaactg gatgctctgg tgcttccgta caaacgcgat ggtgagttgt
     4681 tgcaggtcaa gcgaatcagc actgagcgcc cggacgggaa gaaagtcatt atggcagaag
     4741 gtgattgcga accttgtctg ttcggatggc aggctctgga cgctggcgtg agggcggttg
     4801 tactttgcga aggcgaaatt gattgtatga gctatgcgca atacggcatc tcggcgttat
     4861 ccgtgccgtt tggtggcggg aaaggcgcta agcaacagtg gattgagttt gagtatcaca
     4921 acctcgacag gtttgaggaa atattcatct cgatggacgt tgatgatgtt ggtcgtgaag
     4981 ccgcaaggga aatcgcaagc cgactcggtg aacatcgttg ccgtcttgtt actctgccgt
     5041 acaaagacat caacgaatgc ctgatgaacg gtgttaccga ggatgaaatc tggcagtaca
     5101 tcggcacggc atcctacttc gatcctgaag aactctacag cgcgcgagag ttttaccagg
     5161 acactatcaa cgccttctac ggcaagcagc agtatctgtt taatccaccg tgggaatctc
     5221 tggcagataa attccagttc cgtgaggccg agttgacgct ggtcaatggt gtgaacggtc
     5281 acggaaaaac ggaggttgtc gggcatatgg cacttgaggc aatgcgtcag ggtgtgaaga
     5341 cgtgcatcgc gtcacttgag ctgaagcctg gtattctcct taagcgcctt acccgtcagg
     5401 caacgtgctg caagatgccg ccagtgcttg aaattgactc tgcatttaaa ttctatgacg
     5461 aaagactctg ggtgtttggc ctgactggaa cggcgaaagc cgacaggctg atcgaaatat
     5521 tcgactacgc tcgccgccga tacgg
//
LOCUS       DAZFZC010000195         5399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFZC010000195 DAZFZC010000000
VERSION     DAZFZC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.197"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..173)
                     /locus_tag="ACHKXP_004951"
     CDS             complement(<1..173)
                     /locus_tag="ACHKXP_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7649756.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRY"
     gene            complement(242..3721)
                     /locus_tag="ACHKXP_004952"
     CDS             complement(242..3721)
                     /locus_tag="ACHKXP_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649757.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVEGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQCCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTRRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVLFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPDGRLTAKNADISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTVG
                     KAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERENNDIYSSCRLI
                     VKKNGAEIYNRTALDNTLVYTGVIDMPAGRGHMTLEFSVSAWLVNDWYPTASISDLLV
                     VVMKKSTAGITIS"
     gene            complement(3782..4384)
                     /locus_tag="ACHKXP_004953"
     CDS             complement(3782..4384)
                     /locus_tag="ACHKXP_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7649758.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPSFRQKLNE
                     GWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(4321..5064)
                     /locus_tag="ACHKXP_004954"
     CDS             complement(4321..5064)
                     /locus_tag="ACHKXP_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7649759.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPERERYFPCVNISGEPE
                     AYFRMAPEDWLRAEMQGEIVALVHSHPGALPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            complement(5070..>5399)
                     /locus_tag="ACHKXP_004955"
     CDS             complement(5070..>5399)
                     /locus_tag="ACHKXP_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7649760.1"
                     /translation="DADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRI
                     MLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFL
                     SINKLSQ"
BASE COUNT         1140 a         1713 c         1346 g         1200 t
ORIGIN      
        1 tcataacggt atttcacgtt aatcccgttc agatcatcac tgccgggaac gttcgtccgg
       61 gcatgaagat accccgcgct cagcgtggac tgatgctcag acgcccatgc aggcgcaccg
      121 gatacggcca gacagatggc tgcggacaaa atggctgcac aaactttacg cataattacc
      181 tctcgctttt ctgcaataaa aaaggcgcca gaaatggcgc ccgcatatgg gttatgaaaa
      241 ttcagctaat cgtgatacct gctgtggatt tcttcatcac cacaaccagc aaatcactga
      301 tactggctgt gggataccag tcatttacca gccacgctga taccgaaaac tccagcgtca
      361 tgtgaccgcg accagcaggc atatcaataa cacctgtata aaccagcgta ttatccagcg
      421 cggtacggtt ataaatttca gcaccgtttt tcttcactat caggcggcat gacgaataaa
      481 tatcgttatt ctcccgctca tgtttagcgc cacgaaacgc caccgcggga ataacaatct
      541 gccggtcaaa cggctgatcg tcataaaccc tgacggtaat ggtccctgat ggccaccgct
      601 ccggtgcacg ggagtcccgg gggaaagctt tgcccactgt tttaacgaga tcgccttcaa
      661 tctggttcgc agacagtttt cccagaaccc gacagttttc atttatcgtg acattgttga
      721 gcgtcccggc gttcgcattc acactgccac tgatatccgc atttttcgcc gtcagtcgcc
      781 cgtccggtgt cagggaaaat gccggtggat ttccaccgct ggtaatggtg ggggccgtca
      841 ggcgtttcag gaacacgtcg ttcatgaata tctgatcgcc ctgaccaaca aacatcggtt
      901 ttgtgttgcc attcgcagga ttaaccatcg caatcctgtc cgccgccagc agcacctgac
      961 tctgcatgcc gtcaggggtg ttctcaatac cggcaccaat acccgcgata taaaggcgtc
     1021 cgtcctgcat ctgctgcagc ttcacagccc acatgctgtt caggttatta tttgtatcaa
     1081 cctgaacctt ctgtatctgc tgaattgccg cactctggtc ttccagtttc ttattgacgg
     1141 tctgcgtgat ttcattgctg acatccgtaa tggacgtcct gatttcagcc aggtcaggcg
     1201 caagctgacc gttatcaatc tgcgtccaca gctcctgagc cagatgggtt ttccctatct
     1261 cgcctttgaa aaaatccaga tagccggatg catcatcgct cgcccgaccg acggcctcca
     1321 caaatgccga tttgcctacg gtgttcacac tgcggatata aaagtaataa tcatggcccg
     1381 gtttgatatt gatactggcg gctatccagt acagcgccgt accaagatag cgggctgtgg
     1441 tttcaacctg cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg
     1501 tcggatcata aaccgcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct
     1561 caatccgcga cggcgctgcc ggtgcggcga tccggaacaa taccgatgcc ggatcgccct
     1621 gctgtcccca tgcatttacc gcccggactg tcagcctgta atttcccagc gccagctgcg
     1681 tgaagcgata tgtggtttcc gtcgtccggg ccgtgctgac caaccgctca ctgccgtcgt
     1741 ccgctgccac ggtcaggcga agcataaagc tcaccccctt caccaccttc ggcgtgtccc
     1801 agcgcgccag cacctggtat tccccgctgt ctgcggtgac ttctgcggtc aggtgctgaa
     1861 ctgctggcgg cgtgacacca ttcaccgtgc cgctctggtc accgtcaaag tgcgccccgt
     1921 tatccacgat ggcttctttc tccggtacat gctgcacggc agtgatggca tacgtaccgt
     1981 catcgttctc acggatactc acacagcgga acaggcgctg acgcagcgtc ggcagcttca
     2041 gcccccacac gctgtactcg gcaacgccgt caggaacccg gctcactttc accttcacgc
     2101 cgtcggtgac ggactggacc tccacgctca ccggattacc ctgcccgtca accaggctta
     2161 tcagcgtggt gccggaggat ggcagcgtga tttcacggtc gagcgtcagt gtccgcgtct
     2221 ggctgttcac cgccagcacg cgcccgccga tgctgatccc cgcatagtca tcatcacaga
     2281 tttcaatgac atcacctggt acatggcgaa gcccttcggc acccacgctg aagtccacgg
     2341 tctgcgtttc cagcagttcc gttttaatca gccacagccc ggctcggtgc gcctgccccc
     2401 gcctggtaca gccaaaggca tccatcttcg tgacgttacg accgtaacgg gcaatggcct
     2461 gcgtgtcctc cacaagctct gtcgccgtct cccagccgtt atccgggtca atccagttca
     2521 cctcaacggc attatggcgg tccttcaggg cgctgaagct gtagcggaac ggcgcgccat
     2581 catccggcat caccacatta ctgcggttat aggtccacac cttatctgat ggtcggtcct
     2641 gcacgaacgt cagcgtctgc ccgttccata ccggcataca gcgcatcgcc gagcagaaat
     2701 cactgagaac atcccacgcc ttacgctgtg tggtcagcca ggcattacag gtgatgcgcg
     2761 gctccgtgcc gccaaaaccg tccggcaccg actggtcgca acactggccg atgacataca
     2821 gcgcccattt atccacatct gccgcaccaa gacgtttacc catgccgtag cgcgggtggg
     2881 tcagcatatc ccacagacac caggccatgt tgttgctgta tgctggctta aacgttccgt
     2941 cccagatacc gctgtattgc cgcgtctgcg ggttatagtt cgacggcacc tgcagaatac
     3001 gcccgcgaag atgataatta cggctcacct gctggctgcc gaactgctcc gaatccacct
     3061 gtacgccgac cagtgccgtg ttcgggtagc actgtttcac atcgatgatt tcggtgtatg
     3121 acgaccagag cgttttgttc tgcagctggt ctgtggtgct gtccggcgtc attctgcgca
     3181 tccggatatt aaacgggcgc ggcggcaggt tatccaccac caccgaggcc agatactgcg
     3241 aggtggtttt gcccttaatg gtgatgtctt tttccgtcac ccagccaccg ttacgctgta
     3301 tctgaaccag caggcgaact tccgacggat tccggtcccc ctttgaggtg gtttccacca
     3361 gtgcctgcac gccgaaggta aaacgcagtc ggtcaatgtt tgccgacgtg atggtccggg
     3421 tgatcggcgt gtcgtatttc acttccgtac ccagcaccgt ctcggagccg gaggattcaa
     3481 atccctccgg cggtgtctgc tcctgctcac cggcccggaa caccaccgtg acgccggaga
     3541 tgttggtatt cccctcagtg tccagcaccg gcgtactgtt cagcagcaca ctttttaatc
     3601 cctccaccgg accttcaatc ggcccttcgc tgatggcatc gatcacactc agcaactgcg
     3661 tggacttcag gttgtccttc gcttcgcgcg gagtatgccc cttactgctt cctttaccca
     3721 ttcctcacgc tccataaacg acaaaaccgc ccgcaggcgg tttcacataa aacattttgc
     3781 atcagcgacc aatcaccaca acctgaccac catccccttc atctgctgtg ctgatctcct
     3841 gagataccac ccgcgacccc acacgcattt caccgtacag aacgggcagg acattgccct
     3901 gggcaaccat attatccagt gaagaaaaat aggtgttctg tttgccgtta tccgttgtct
     3961 gtgtgcgggg agttctggct ttcggtgcca gcatctgcgc cacaccaccg agcaccatac
     4021 tggcaccgag agaaaacagc agattactcg ccataattcc tacccccggc atccatatag
     4081 caaccgccat aacagccgcc cccagcacag cctgaaacac accgccactt ttggctcctg
     4141 ccagacgcgg cacgatatgg atcacagcac catttgccag cggttcatta agacgggctg
     4201 ataattcatt ttcgcctgca tcacgcccgg caatgcgcac ctgataccag ccctcattca
     4261 gtttctggcg aaacgacgga agctgcgtgg ccagcgcccg gatggcttcg gcccccgttt
     4321 tcacacgaag gtcgatgcgg cggccaaatc gttgtaaatc cccgtaaagg cagatgcgtg
     4381 ccatgcccgg tgacgccaga gggagtgtgt gcgtcgctgc catttgtctg tatacctctc
     4441 tcgtttgctc agttgttcag gaatatggtg caacagctcg ccgtcgccgc agtaaatggc
     4501 ggcatgattc ggcaccgatg aaccaaaaca gcacagcagc acatcgcccg gctgcgccgc
     4561 tgacaacggc acctgataca gccctgtggc ctccagatta tccagataga gattctgacc
     4621 gtgacgccac cagtcatcct cacgatgaaa atcaggcatc tcaattcccg ccagatggta
     4681 agcatcccgg aacagcgtgt aacagtccgt caccccgtgc tcaaagcgcc gcccggtaag
     4741 atgtggcaca cagcggaact tgtgaatcgc cccccggcag accagccacc acggcaaatc
     4801 actctgcacc tgcagccgcc ggtcggcctc actcagccag ggcagagcac cggggtgact
     4861 gtggaccagc gccacaatct caccctgcat ttctgcccgc agccagtcct ccggagccat
     4921 ccggaaatac gcctccggct caccggagat attcacgcag gggaaatatc tttcccgctc
     4981 cggcgtgctt accacgaagc cgcacgactc cgctggcgca catcgccggg cgtgcgccag
     5041 aattgctgat tctgtctgtg tcatgggatt tactgcgaaa gtttgttaat ggaaaggaag
     5101 ccgccaaagt tgccgacgtt attgcggaac ttacaaccgc tcaggcattt gctgcattta
     5161 tccttcgtga tatcggacgt tggctggtca tattcatccg cgaccgccgg accgtgataa
     5221 ccgcactcat cgccgcgata ggtccaggtg caggtattag ccagcatgat gcggcccgga
     5281 aaaacggcac catccgtttc cgtcggtgtg gagagcacaa aggaagcact gaccgcgctc
     5341 agttcgctgc actgctcgat gcgccagcgg ctgatcacct cctgctccgg atcggcgtc
//
LOCUS       DAZFZC010000196         5347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFZC010000196 DAZFZC010000000
VERSION     DAZFZC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.198"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..1451
                     /locus_tag="ACHKXP_004956"
                     /pseudo
     CDS             99..1451
                     /locus_tag="ACHKXP_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1464..2423
                     /locus_tag="ACHKXP_004957"
     CDS             1464..2423
                     /locus_tag="ACHKXP_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO7649761.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNAFRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVVYN"
     gene            complement(2461..3360)
                     /gene="nhaR"
                     /locus_tag="ACHKXP_004958"
     CDS             complement(2461..3360)
                     /gene="nhaR"
                     /locus_tag="ACHKXP_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO7649762.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3426..4592)
                     /gene="nhaA"
                     /locus_tag="ACHKXP_004959"
     CDS             complement(3426..4592)
                     /gene="nhaA"
                     /locus_tag="ACHKXP_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO7649763.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5178..5330
                     /gene="mokC"
                     /locus_tag="ACHKXP_004960"
     CDS             5178..5330
                     /gene="mokC"
                     /locus_tag="ACHKXP_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO7649764.1"
                     /translation="MKQHKAMIVALIVICITAVVAVLVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1635 a         1191 c         1135 g         1386 t
ORIGIN      
        1 ttcttataaa gaagggaaca attaagaata ttaaattttc aaactttcat caacttcaca
       61 tcaagaaatt aaaaaactta aacattaatc taaataataa caacctgatc acataatctg
      121 gtacgatttt atccgataac tataatgcta taactctggg aaatggttgg aatacaccgc
      181 tgggtgccat ctcttttgac gctacccgat cgagtagtaa gttaaataat gataccagac
      241 acgaaggtac gagctatcag gtcgcctata ataaatatct attgcaaacg gccactcact
      301 ttagcgtggc cgcctggcgt tatgcttcgc aggattatag gacatttagc gaccatcttt
      361 acgaaaatga taaaataaat catcagagtg actatgacga tttttataat attggcagaa
      421 aaaatagcct ttctgccaat attatgcaac ctttatctaa taatctggga aatgtatcat
      481 taagtgcgct ttggcggaat tactgggggc gaagtggaaa tgctaaagat taccaattca
      541 gttattccaa tagctggcaa cgcatcagtt atactttctc tgccagccaa tcctatgatg
      601 aaaatgacaa agaagaggag cgttttaatc tgtttatctc cattcctttc tactgggggg
      661 atgatattgc caaaacacgt caccaaatta acttatcgaa ttcgacctca ttttccaaag
      721 atggctattc ctccaacaat actggaatta ctggcatagc cggtgaacat gatcagttaa
      781 attatggtat atatgttaat cagcaacaac aaaataatga tacatcgctt ggtacgaatt
      841 taagctggag aactcccatc gccataatag atggcagcta tagtcattct aaaaacgcct
      901 ggcaaagtgg tggaagtatt agtagtggat tagttgtctg gtccggtggt attaatatca
      961 ctaaccagtt atccgataca tttgcaattc tggatgcgcc tggattagaa ggcgcgcata
     1021 ttaatggaca aaaatacaac cgaacaaaca gcaaaggcca ggttgtttac gacccgatta
     1081 tacctcatcg tgaaaaccat ctggtacttg atatagcaaa cagtgaaagt gaaacagaat
     1141 tgcagggcaa tcgtcaaatt attgcgcctt accgtggagc agtttcttat gtgcagttta
     1201 caactgacca acgtaagccc tggtatatac aggcactgcg tcccgatggt tcgccattaa
     1261 cctttggcta tgacgtactg gatctccagg aaaacaatat tggagtcgtt ggccagggta
     1321 gtcgcctttt tattcgcgta gatgaaattc caactggcat aaaagttgct ctcaatgatg
     1381 aacagaattt attctgtact attacttttc aacacgttat cgatgaaaac aaaacatata
     1441 tatgccagta aaaggagttt actatgaagt ggcttttatt aatcaccttg agtctatatt
     1501 cttttattgt gcaaagtgct ccctgtggat tgaccaatgt cggggaacaa cgtggaacat
     1561 atatactcaa gccactgagt atgaaaggta atctcaccgc cgaaaaaatt gaaaacaccg
     1621 gaaatatcat tgagtgtgca gacttacaag agacgaccaa cgccattaaa gttcattttt
     1681 taacacagaa cgcttttagg tttaaagtcg gaagtcgtca ttatattatt aatttcccct
     1741 ttgaatctga cggtaaaaaa atagagcttc caggaaggtc atcttataat ttagaagaaa
     1801 tgctatctgc gataaatttc accattccat actctatcgc atctgtatca gatagttcca
     1861 acaccgcaga tatttttcca ggaactcctt ttcctttgtt gttcgaaatt gaagtgagtt
     1921 catgtacagg gaataatact aattgtaata gcgtccgctt taattataat ttaaatacta
     1981 ttttacaggt aaatttaatg acctgtggtt ttgaagatca atccatcgac actggacgat
     2041 ttgtactgtc tcgtatcaat aatgaaaatt atcagtttca tcatataaga tttgactgca
     2101 tccagggcga gcaaggtgaa ttagtttttg aacccagtga tgttgaatat tactttgaac
     2161 cagtgacgat acaggagtca ggaaccagca tattaaaaaa tgagttagaa aacagtgagg
     2221 acggtgcggg tcaagtaggc tttcaactct ccaatgatgg tacgaatgaa atccagtatg
     2281 gtaaaagcaa ttactactct tttcagcatc cccatgaagg aagtaatcag attcctctat
     2341 ttattcgccc gcgtacatat ggaaataatg tctcaagcgg gcaaataatg agccgagtca
     2401 aaatcgtggt tgtgtacaac taatctatct cctccacttt aactttgggg gagctgccga
     2461 ttaacgcgcc gctggactaa aaagcgcaga ataatccgta ttgcagattc gctgtaccgc
     2521 cgggtgctga atcatccgct cagcaaaaat agcatggtac tcttccatca cattctcgac
     2581 gcgaccaatt tctacgacag ttttatcggc ataaaagtca tatgcataaa gcgttggggc
     2641 aacgaagatt gcattgtgca ttgcaccaaa agctttcatc aacgcggcat catcaaactc
     2701 gccgaggatt tctacgttta atccctggga gttaaaccag ttaagcaatt tgcgccctaa
     2761 cattgaacgt cgcccaggaa tcaaaagtcg ccgttcttcc agacaagccg ggaacggttt
     2821 ttctggtggt ggatttgtac accagaaact cacgccacat tcgccaattc tcacggagaa
     2881 caggccttcc tgctgcgtag agtctatcgg acagtcggaa atgatcatat ccagtttatg
     2941 ctggcttaat tgctccagta gcatttcgtg ggtggattcg aagcagcgaa gatgaatcgg
     3001 ctcgccttct accactgcag cattcagcac gctactgacc aggcgtttgg aaagtgcatc
     3061 agccacgcca acgtcaaaca ataaattgga ttctttgcga tagttcacaa tatccagcat
     3121 ttcctggctt aaggtgaaca ttttatcggc atagcgatag accagttctc ccagctcgct
     3181 gggttcgaga ccacgtccct tgcgcttaaa taatttgcct tgcaggcgct cttccagagc
     3241 ccggatctgt ccggtaatgg tttgtggagt taaataaagc gcctccgctg cgccaaccac
     3301 ggaaccttct ttatagacat gccagaagta atacaagtgg ttgtaattga tatgagacat
     3361 gctcatttct ctccctgata acaatgaaaa gggagccgtt tatggctccc cggtaaaccg
     3421 tcctgtcaaa ctgatggacg caaacgaacg cgtaaccagc tgtatccaat taccgccgaa
     3481 gagatagaac cgaccaggat accgagtttc gcccagttaa tcagttctgg atctacgcta
     3541 ccaaaggcca ggctggcaat aaagatagac atagtaaaac cgataccgca caggatcccc
     3601 accgccataa tttgctgata agtcgttccc tcaggcagat gcgccagttt caaacgcagc
     3661 gccaaccagc agaacagact aatccccagc ggtttgccaa tcagcaagcc agcgatgatc
     3721 cccaatggca gaatggaggt caagccatcc agcgtgacac cttgcagtga aacgccagca
     3781 ttagcaaatg caaacagcgg caaaatcaga tacgccaccc acgggtgtaa cacatgctcc
     3841 agtcgcttcg ctggagaacg cccatgcttc tctttcaaag gaataaagaa gccgacaatt
     3901 acccccgcca gggttgcgtg aacccccgat ttcaacaccg cagtccacaa cactacgcca
     3961 accagaatat agacgcccgt gcggcgtaca ccacacagat tcaataccgc gagtaccgca
     4021 attgctacag ccgcgacgcc aagagaggcc atcgataagt cattagtgta gaacaatgcg
     4081 atgataatga tggccccaag atcgtcgata atagccagag ccatcaaaaa gatcttcagc
     4141 gctaacggaa cacgacttcc caacagcgcc agcacaccaa gtgcaaaggc aatgtcagta
     4201 gccgccggga tcgcccaccc ttcgcgggta atcggatcgg catagttaaa ggccagatag
     4261 agtaatgccg gaacaatcat cccaccaata gcggcgataa ctggaaatgc ggcctggcgt
     4321 aagctggcta gcgatccttg catcagttca cgtttaactt ccagaccgac taacaggaaa
     4381 aataccgcca tcagcgcgtc atttatccat aacagcatgt ttttgttgat ttcgagtgaa
     4441 ccaacccgga gctgaaccgg cgtctccaga aagtcgtgat accatccact ggttgcgccg
     4501 ctgttggcca tcatcatcgc caggatagcg gcaatgataa gaataatgcc tcccgaggca
     4561 tcactgctaa agaatcgatg cagatgtttc actttttatt tctctttcag gtgaatagat
     4621 cgtttttact attttacccc gcacgaatca tcgttaaaat cagattatag tgtaggaata
     4681 actcgttttt tacgagcaat taaaggattt gcgccatcag cggtgagatg agtttttgac
     4741 ggctgtaagc gctaacgggt gaccctaagc cctgataatc atagcttaag gtcaggattt
     4801 ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt tatttgttag
     4861 gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaaga gataaattaa
     4921 agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt cttcaggatt
     4981 agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg tgcttgaggc
     5041 tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg atattttact
     5101 atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact gaccgtaagg
     5161 tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg atcgtcatct
     5221 gtatcaccgc cgtagtggcg gtgctggtaa cgagaaaaga cctctgtgag gttcacatcc
     5281 gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa gagcaacggc
     5341 ggggagt
//
LOCUS       DAZFZC010000197         5319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFZC010000197 DAZFZC010000000
VERSION     DAZFZC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.199"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..352
                     /locus_tag="ACHKXP_004961"
     CDS             <1..352
                     /locus_tag="ACHKXP_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649765.1"
                     /translation="PELKNRLHCWLVFSPVYFWLILSNFILYISPEKSALLENIRNFF
                     LTFVWLPLNFSLFWPQPWTDFVGPISAQLGFALGYYCHWCRKNRNQRKKWGDWVTCLS
                     LAILAQGPLFNYLQ"
     gene            complement(406..1911)
                     /gene="glpD"
                     /locus_tag="ACHKXP_004962"
     CDS             complement(406..1911)
                     /gene="glpD"
                     /locus_tag="ACHKXP_004962"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO7649766.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2101..2427
                     /gene="glpE"
                     /locus_tag="ACHKXP_004963"
     CDS             2101..2427
                     /gene="glpE"
                     /locus_tag="ACHKXP_004963"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO7649767.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2472..3302
                     /gene="glpG"
                     /locus_tag="ACHKXP_004964"
     CDS             2472..3302
                     /gene="glpG"
                     /locus_tag="ACHKXP_004964"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO7649768.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3319..4077
                     /locus_tag="ACHKXP_004965"
     CDS             3319..4077
                     /locus_tag="ACHKXP_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO7649769.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4059..>5319)
                     /gene="rtcR"
                     /locus_tag="ACHKXP_004966"
     CDS             complement(4059..>5319)
                     /gene="rtcR"
                     /locus_tag="ACHKXP_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO7649770.1"
                     /translation="TGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGPGEVTII
                     DLDLSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNG
                     PTGAGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARES
                     REGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTV
                     RDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRF
                     NTEARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNW
                     QESRPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASV
                     NDADRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1187 a         1413 c         1437 g         1282 t
ORIGIN      
        1 tcctgaatta aaaaacagac tgcattgctg gctggtcttt tcgccggtct atttctggtt
       61 aattctctcg aatttcattc tttatatttc tccagagaaa tcagcgttgc tggaaaatat
      121 ccgaaatttc tttctgacgt ttgtctggct tcccctgaat ttttcccttt tttggccgca
      181 gccttggact gattttgtcg gcccgattag tgcacagctt ggttttgcat tgggatatta
      241 ttgccattgg tgcagaaaaa atagaaacca gaggaagaag tggggcgatt gggtaacgtg
      301 cttaagtttg gcgattttag ctcaggggcc gttattcaat tatttacaat gaatagtcta
      361 aggaatccaa atagcccggt tgcacttcac aaccgggcat catccttacg acgccagcga
      421 caacttctgc tgcgtatact ccaccagcca ctgactcaca cgagattgtt gatccgcatt
      481 cagccacatg ccttgttttg tgcgacgcca cagggcgtcg tcggcgcggc ggacccattc
      541 gtgatccacc aggtatttca gctccgcttc gtagaactca tgaccgaaat cttccccgag
      601 atcgcttacc gttcccgcat tgccgagcag cagctcgctg ttgctgccgt aagtgcgagc
      661 gtaatgacgc gccagcgatt cagtcaggaa cggatagcgg cggcgcaggc gagcggcata
      721 atcgtcgcgg tcgccttcaa tggcgccacc cggtagcaca ctctctttcg tccatgccgg
      781 gccaataccc tgataatacg gcgttagttt ttccagcgca tgttccgcca gttttcggta
      841 ggtggtcagc ttaccgccga ataccgacag cagcggtgct ttgccatttt catcatgaat
      901 atcaagggtg taatcacggg taatagcctg cggcgagtcg gactcatcat cacacagcgg
      961 acgcacaccg gagtaggtcc agacgatatc gtcacggctt aactgctttt taaagtgcgt
     1021 gttatacaca ttcagcaggt aattgatttc actctcttca atcttcaccg ctttcggatc
     1081 gcctttgtac tcgacatcgg tagtgccgat gatggaaaac tcgtccatcc acgggatcac
     1141 gaacacaata cgtttatctt cgttttgcag aatgtaggct tgcttctggg tatgcacgcg
     1201 cggcaccaca atatggctgc ctttgatcag acgaatgcca taaggcgaag gcagatgcat
     1261 cccgtcgtcg aagaactgtt tcacccacgg gccggtggcg ttaaccaagc cgcgcgcttg
     1321 ccagctatat tttttgccgg tatcgatatc ttccgcttcc acaatccaca ggccgttttc
     1381 gcggcgagca gaggtggcgc gagtccgagt aagcacttcg ccgcctttac gcaccaccat
     1441 ctgggcgttg gcgagtacca gacgggcgtc gtctacccaa cagtcagaat attcgaatcc
     1501 gcgcttaatt tccggtttta acactgaatt tgcgccaaaa cgcaaaccag ttgatcccgg
     1561 caagctggtg cgtttaccca gatgatcgta cataaacaga ccaatgcgaa tcatccacgc
     1621 cgggcgcaga tgcggacgat gtggcaggcg aaaacgcatc gggaaggcga tatgcggggc
     1681 cattttcagc agcacttcac gttcagccag cgcctcgctg accaggcgga attcatagtg
     1741 ctcaaggtag cgcaggccac cgtgaatgag ttttgaactg gcggaagagg tcgcgcaagc
     1801 gagatcctgc gcctccagca tcagcacgga taaaccgcgt ccagcggcgt ctgccgcgat
     1861 accagcacca ttgatgccgc cccctatcac aatcagatct ttggtttcca tgctgccctc
     1921 attcactttc gttaaagctc ataaatgttc gttatcgaac atattagcaa agaatcgcgc
     1981 tttaggtaac attgaaaaaa cattttagag tgatatgtat aacattatgg cgtttatctg
     2041 ccgcttcgac gtaaactgtg cggtaaattt gcccacttgt ttgtaaagaa agagagacgc
     2101 atggatcagt tcgaatgtat taacgttgcc gacgcgcacc agaagttgca ggaaaaagag
     2161 gcggtgctgg tcgatattcg cgatccacag agtttcgcaa tgggacatgc ggtgcaggct
     2221 ttccatttaa ccaacgacac gctgggcgct tttatgcgtg ataacgactt tgacactccg
     2281 gtgatggtga tgtgttatca cggcaatagc agcaaaggcg cggcgcagta tctgctgcaa
     2341 cagggctacg atgtggtcta tagcattgac ggcggctttg aagcctggca acgtcagttt
     2401 cccgcagagg tggcgtacgg cgcgtaacgc tttatactgt ccccttttgt gtggaataag
     2461 cgacagcaac gatgttgatg attacctctt ttgctaaccc ccgcgtggcg caggcgtttg
     2521 ttgattacat ggcgacgcag ggtgttatcc tcacgattca acaacataac caaagcgatg
     2581 tctggctggc ggatgagtcc caggccgagc gcgtacgggc ggagctggcg cgttttctcg
     2641 aaaacccggc agatccgcgt tatctggcgg cgagctggca ggcaggccat accggcagtg
     2701 gcctgcatta tcgccgttat cctttctttg ccgccttgcg tgaacgcgca ggtccggtaa
     2761 cctgggtgat gatgatcgcc tgcgtggtgg tgtttattgc catgcaaatt ctcggcgatc
     2821 aggaagtgat gttatggctg gcctggccat tcgatccaac actgaaattt gagttctggc
     2881 gttacttcac ccacgcgtta atgcacttct cgctgatgca tatcctcttt aacctgctct
     2941 ggtggtggta tctcggcggt gcggtggaaa aacgcctcgg tagcggtaag ctaattgtca
     3001 ttacgcttat cagcgccctg ttaagcggct atgtgcagca aaaattcagc gggccgtggt
     3061 ttggcgggct ttctggcgtg gtgtatgcgc tgatgggcta cgtctggcta cgtggcgaac
     3121 gcgatccgca aagtggcatt tacctgcaac gtgggttaat tatctttgcg ctgatctgga
     3181 ttgtcgccgg atggtttgat ttgtttggga tgtcgatggc gaacggagca cacatcgccg
     3241 ggttagccgt gggtttagcg atggcttttg ttgattcgct caatgcgcga aaacgaaaat
     3301 aattccaggg atttataaat gaaacaaaca caacgtcaca acggtattat cgaactggtt
     3361 aaacagcagg gttatgtcag taccgaagag ctggtagagc atttctccgt cagcccgcag
     3421 actattcgtc gcgacctcaa tgagctggcg gagcaaaacc tgatcctgcg ccatcatggc
     3481 ggtgcggcgc tgccttccag ttcggttaac acgccgtggc acgatcgcaa ggccacccag
     3541 accgaagaaa aagagcgcat cgcccgcaaa gtggcggagc aaatccccaa tggctcgacg
     3601 ctgtttatcg atatcggcac cacgccggaa gcggtagcgc acgcactgct caatcacagc
     3661 aatttgcgca ttgtcaccaa caatctcaac gttgctaaca cgttgatggt aaaagaagat
     3721 tttcgcatca ttctcgccgg tggcgaatta cgcagccgcg atggcgggat cattggcgaa
     3781 gcgacgctcg attttatctc ccagttccgc cttgatttcg gcattctggg gataagcggc
     3841 atcgatagcg acggctcgct gctggagttc gattaccacg aagttcgcac caaacgcgcc
     3901 attattgaga actcgcgcca cgttatgctg gttgtcgatc actcgaaatt tggccgtaac
     3961 gcgatggtca atatgggcag catcagcatg gtagatgccg tctacaccga cgccccgccg
     4021 ccagtaagcg tgatgcaggt gctgacggac caccatattc aactggagct gtgctgatcc
     4081 tgcacggctt cccacgtcag accaaaacgc gccaggtatt tgcgtagccg atccgcgtca
     4141 ttgacgctgg ctttgccctg gcgcgaaacg tcaaaaagct ggcgtccggc tgcggaaagc
     4201 gactttgcct ggcggcagat agcgataacg tgttccagtt gcatacggtc gaagagatcg
     4261 atgttttctg cctctgcgcc cagcaacgcc gtaagcgcgg aggggcggct ctcctgccag
     4321 ttatagcgca gacggtttat ctcatcttca accacgtcca gagtgatgcg tccgctagtg
     4381 gcaaaggtgg ccatccgcgt gacgctggca gaaagttcgc gaaagttacc gcgccatgtt
     4441 gcctggggag aggtcgcaaa agccaaccag gcgcgccgcg cttcggtgtt aaaacgcacg
     4501 ctgtcgccag tgagtgaggc gtggcgctcc acttcataat ccaggttcgg ttcaatatct
     4561 tcctggcgct ggcgtagacc cggcagggtg aaggtccaga gattgatccg cgcgtacaga
     4621 tcttcgcgaa atttgccttc ggcaaccagc tggcgcaaat cgcgcaccgt tccggcgata
     4681 agctgaaaat cactgctcac ctggcgatcg ctgccaaacg ggtaaaaggt tttctcttca
     4741 atggctttca gcagcattgc ctgttcgtct gcgcccagtt cgccaatctc atcaagaaac
     4801 aacattccgc cgttggcgct gcgtaataaa ccttcacgag attcccgcgc cccggtaaac
     4861 gcgcctttta catgaccaaa cagcgtcgac atggcggtat cgccgcgcag ggtggcgcag
     4921 ttcacttcca caaacgcgcc gctaaactga tgccgcgcct gttttaactc gaagatgcgt
     4981 cgcgccagaa atgacttgcc cgcgccggtt ggaccgttaa gcagaatcgg cgcgcgggat
     5041 ttgatcgcca ctttttcgat ctgctcaatc atgcggttga agtgggggtt acgcgtggca
     5101 atgccggact taagaaaatc aagcgtttgc tggcgttcct cggcaaagcg gctggcgatg
     5161 gcgttataac ggcttaaatc gagatcgata atcgttactt cacctgggcc gcgcggctgt
     5221 tcttttttgc gcggaggtga agattgtatc agccgggcgg gcaggtaacg cgcttctgcc
     5281 agcagaaacc agcaaatctg cgcgacgtgg gtaccggtg
//
LOCUS       DAZFZC010000198         5307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFZC010000198 DAZFZC010000000
VERSION     DAZFZC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.200"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2672)
                     /locus_tag="ACHKXP_004967"
     CDS             complement(<1..2672)
                     /locus_tag="ACHKXP_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7649771.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMQSQRAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLDDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTCKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVTVTGADGYYNATLDYGDGCTTVTDGKGIHCYYYDPD
                     GNILREAAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWL
                     YRYDERGLLSNITDPAGQTWTQLCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAETGAEGFRYDRHGNLLAYTDGNGVVWTMEYG"
     gene            complement(2692..3153)
                     /locus_tag="ACHKXP_004968"
     CDS             complement(2692..3153)
                     /locus_tag="ACHKXP_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO7649772.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDVKQDQPR"
     gene            complement(3181..5082)
                     /locus_tag="ACHKXP_004969"
     CDS             complement(3181..5082)
                     /locus_tag="ACHKXP_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7649773.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMGRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1004 a         1580 c         1452 g         1271 t
ORIGIN      
        1 ccgtattcca ttgtccagac aacgccgtta ccgtccgtgt acgccagcag gttgccgtgg
       61 cggtcatagc ggaatccttc tgcgccggtt tctgcccgct caaggccgct cagtttatgg
      121 tgctgctgcc actgtaaccg cactgactga ccgccggggc cgcgcatctt caccggcagg
      181 tcgtgaccgc tgtattcggt atggtgttca cggcccatca cgtcggtgag tgtttccggc
      241 cggttgtggt ggtcgtactc tatgcccaga cgccgttcat cctggcggaa tattcccgac
      301 aacagcccct gagcggtgta cgccagccgg gtctcttcgc cctgtggcga caccaggctt
      361 accggcaggc cgcgttcatc gcacagctgt gtccacgtct gcccggcagg gtcggtgatg
      421 ttgctgagca ggccgcgctc atcatagcgg tacaaccact ccgcgccgtc tgccgccgtg
      481 taacggctta actgaccgtg tgcggcgttg tattcaaact tctccacccg cccggcaggg
      541 gagtggcggg ccagcaggtg atggaattca tcccattcat acgtggtggt gctgccgtcc
      601 ggcgctgctt cccgcagaat attgccgtca ggatcatagt aataacagtg aatgcccttg
      661 ccgtccgtca cggtggtgca gccgtcacca tagtccagcg tggcgttgta atagccgtcc
      721 gccccggtga cggtcacgca ccggccctgt gcatcgtagg tgaaacggct ccacgtctgg
      781 tcgttatcgg accagcggat gatccggtcc gcagcgtcgt actcataaaa caggtggtag
      841 tccagccgcg catccgcttc tgtcagccgc ccccgcgcat cctgttcgta ggtcgccagc
      901 gtctgtatgc cgttatccgt gcggtgaatg gctttcaggt agccgctggc gtggtacgtc
      961 agtttaagcc ggatcccgtc ggtgtgactg acacccgtga tccggtactg gctgtcgcgc
     1021 tcaaacgtga tgcggttgct ggcggtgtcg ctcatcccgc ccagcatgta catccggtct
     1081 gcggggctgt gtgccaggcg gtcatcgctg gtatcgtcag tcaccgggtg gcgcagacgc
     1141 attcccggaa aggcgtcata aaagaagcgg ctgctcagtg catccctgtc gaacagctcc
     1201 agataatcat cacggcggcg cagaatgtac tgggcgtgat acgggttaac cgccgtatca
     1261 ctttcaccgt cgaaggcgaa gttcagcgtg gctcccaggc tgatgatatc cacccggttt
     1321 ccctgttcac gcacccaggc gtattcgctc caggagtcac tccagcccac gcccgtcagg
     1381 ccatgcagtg gcagtaccga actgtggaaa cgggtgaagg agagggggag ggtctggccc
     1441 agggtgaagt cggtacgttc gtcgcagtac gcgccggtta ccggatcgac aggatctgtt
     1501 acccatctga tgactttatt ggcaatactt tttaacgact tacatgtttt ccccagcaac
     1561 gcgccgagct ttgcgcccag cagcttcccg cttttcccca gcaggtttcc cagtttgccc
     1621 agtttcgcca gattccccag ttttcccagc agtcctgctg gcggcagaag atagctggct
     1681 atcgttattc ctcccaggat cattctctgc catggcggga actccggctg gatatccaga
     1741 taggtgacct gctcaccccc aataaagacg tccggcgaac cttccacgat cgccgcactg
     1801 cactccagtt tgtcgccctt gcgcgcggca ggctgattgt tgataaagac gttgctactg
     1861 ccctgggcta tcctcggtgg tttgttctcc ttgtcgcaga gcgccgtgtc ctcaccggct
     1921 tccgggaaat ccacatcttt tttgatttcg ccacgcccgc ccaccatggt ggcaaactcc
     1981 attaataccg gaccggcggg aaacagaagc gatgcgccgg cgctgacgcg atccataaaa
     2041 ccggcattgc tttccgtcag ggtgataaca gcgttccccc aggccttcgc cagtggtact
     2101 gctttttccg ctgccgattc aagcaggtca gtggcaatat caccccatga gggttccccc
     2161 tgtgggattt caggtattgc tccaggtggg ggaggagggg gaagggtgac ggcggccctt
     2221 gcagcgggct ttccgttaat gtttacattt tcagaaccgg tattgatggc gccgtccgtg
     2281 tcacaaaagc tgtttatcca tgactcaagc tggttctgtt tctcctccac ataatcactc
     2341 gccttatagc tggcatacat cagcgcaggc accagaacca gcgcataagg gccggtggca
     2401 caggcggcca taatcaggca accttctgcg atggtcacta tcgcccccag cacggcccct
     2461 gccagtgccc ccagaaaact cttgtgctga atttcatcgt ccagtctggc ggcaggtggg
     2521 ccggacttct ttgctgccag catctgtgca atggctttgt cgttggctgc ccgtttcatc
     2581 tggctggcga ccatcgcccg ctgcgactgc attcgcattg ccagcgtacc gcctgcggtc
     2641 gctccctgtg gcccgcctgg tccttcactc atgtgttatc cctcggttat gtcagcgcgg
     2701 ctggtcctgt ttgacatcat tctccggtgg caacggggtg aagctcttta tcacctccag
     2761 cagcatcgtc ttttgactgt tcttcaggga taaggaggtg ctgaaagtga aggtcagcag
     2821 cgtatcccca cgccgctgga atacggctat ctggctaatc cgcccctgat tcccttcatc
     2881 ttccggaccg ttccaggaat aatccagcag ccaggccggg gcctctgcca gagtgatttc
     2941 ccggcaggca tgttcggtgt acccatcaag tttgccctgg tacagcgcca gcgtttcctg
     3001 gtacagctct ggtgtcaggg ttccctgtgg aacggcttcg cgggtaatga ccaggttgat
     3061 gccactgtta tctgtggaca ctaggacctg cattgactga tcctgccatt gagacggcag
     3121 ttgaatgtgg ccttcattaa aagtgaactg cataaaatac tccctgataa tctgagtgaa
     3181 tcagttgatg aaaacagaac cgcccacgat ggtgatgcca tccttcgaca gcctgattac
     3241 cgtcgattcc ccgacctgta gctgaatgcc atccggcgtc agcgcaattt tgctttcctc
     3301 cacggccagc aaaatttcct taatcgccgt ataagcctgt ataccgtcgc cttcgccacc
     3361 attgtttaca gcgtcaacat gcaccgaacc ggcaatcgtg cctctgtcct gaaagacatc
     3421 ctcagataaa gaccctttca gtactccggt gattttgtgg cctttttcca ccgtaagaag
     3481 gcggttacct tcctggatcg tgaccgtctg gtcatttttt acggtttcgc gatgatcgtg
     3541 ttttacggtg gtggtacggt cgttgagcac ttcggtatcc atgttcttct gcgcgtggat
     3601 gtagacctgt tctctccctg tcgcatcatc aaacttcagc tcattaaatc cgctgcccat
     3661 gtacgttttg gagcggatgg tcatctgcgt ctttgttccc ggcaggctgc cgggggtgcg
     3721 gttttcctgg tggtaggtgc gccccataat gatcggctgg tccggatcgc cgttgaggaa
     3781 gtctacaatc acctcctgac ccacacgcgg tatcgccagg tggccaaaac cggtgcctgc
     3841 ccacgcctgt gccacacgga tccagcacga actgtcttgg tctgccgggt tataacggtc
     3901 ccagttgaac ttcacccgca cgcgaccatg ttcgtcgcag aagatttcct cgcctgccgg
     3961 tcctgtcacg acagcgctct gcgggccgtc gaccagcggt ttcagcaacg gctgtggtcg
     4021 ccatgttctg tctgccggga tcaccgcaaa atggttctcc agcgccgtcc ccgctccctg
     4081 ccgtcctggc accgcctgtg gctgttcgcc gtgcagttca cttgccacca cctgccattc
     4141 ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt cgccgtcccg gccatatctc
     4201 cggcgagcgg cccattcccc gcgcggtttc tgcgttgttt cgccagccgt ccatctgcca
     4261 gcgggcaaag ttctgcccgt gggcgctctt gaaacgtccg gggtagtcat acacttcata
     4321 ctgcgtgcgc tggtagtcct ggtgctggcc ttcctgttca aaacgtccgg cccagccggg
     4381 gcgtttaaag gtgtagtctt tggtcaccac ggaagaaggg cggatttgtg cgctgtagcg
     4441 gaactggctg atgcagaggg tgctcacctc ggtacgggtg ttcgggttcc atgggatttc
     4501 aaaagattcg ggcagatggc ggactgtgtc gcacagcacc aggctctggt cggtactttt
     4561 gtaagcatgc tcctcataaa agaagatgcc ttcctccgcc gccatccggc acagaaaatc
     4621 gtaatcagtc tcaccgtact ggacacaaaa ctcacgggaa ggatgcggct cgctgaacag
     4681 cggactccat tcggtcaccc cgttttcctg caacatcgtg ccgaggatgc ttttgatgtc
     4741 ctcgttctgg aagatacgga agttctggcg cagaccggca cgccacagcg gcgggtgcac
     4801 cttcatgctg tacagcatct ggtttttgtc gttctccccg agttcaaacc aggtcaccac
     4861 gcctttcacc cggcgctgta cttcatcgcc ctgccatatc gtcaggtagg ccattttgtc
     4921 cagcacctgc gcaaattcaa gggagagaaa ctgctggctg accagggaga gatcgaggga
     4981 aaagagcgaa gagagtgact ggttcagatg aaaggaaacc accgcaaaag catccggcgg
     5041 caggccgtcc acttccagtg tgaaacgtaa tccggttgac atagcaacct ccagtaaaat
     5101 gaataatgat ccgtaccgta caactgcgga taatgtgata agcgggacga cttcagacgt
     5161 tattagggaa ggaacagaaa atgctatggt tatgtgagaa ttttgtttaa atattacttt
     5221 acagaaacgg gtgtctgttc agtcaaaaaa ataatcacaa gctcaacctc ttgtttcttg
     5281 gcgttattca ggacaggtac gcatatc
//
LOCUS       DAZFZC010000199         5210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFZC010000199 DAZFZC010000000
VERSION     DAZFZC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.201"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..295
                     /locus_tag="ACHKXP_004970"
     CDS             <1..295
                     /locus_tag="ACHKXP_004970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649774.1"
                     /translation="IINITDTNQTNILSICNKVLIIQFIAAQPNSPNNNQELLIKIKN
                     IFSEYEIIAISITKMSWSLFHSLLVNIATTFVKHSCPASGILCTLIPQTSGRP"
     gene            292..1266
                     /gene="hemB"
                     /locus_tag="ACHKXP_004971"
     CDS             292..1266
                     /gene="hemB"
                     /locus_tag="ACHKXP_004971"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO7649775.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFAMDLAEKKILR"
     gene            complement(1424..2275)
                     /gene="tauD"
                     /locus_tag="ACHKXP_004972"
     CDS             complement(1424..2275)
                     /gene="tauD"
                     /locus_tag="ACHKXP_004972"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO7649776.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2272..3099)
                     /gene="tauC"
                     /locus_tag="ACHKXP_004973"
     CDS             complement(2272..3099)
                     /gene="tauC"
                     /locus_tag="ACHKXP_004973"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO7649777.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAARSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3096..3863)
                     /gene="tauB"
                     /locus_tag="ACHKXP_004974"
     CDS             complement(3096..3863)
                     /gene="tauB"
                     /locus_tag="ACHKXP_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO7649778.1"
                     /translation="MLQISHLYANYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQNNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3876..4838)
                     /gene="tauA"
                     /locus_tag="ACHKXP_004975"
                     /pseudo
     CDS             complement(3876..4838)
                     /gene="tauA"
                     /locus_tag="ACHKXP_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
BASE COUNT         1241 a         1473 c         1285 g         1211 t
ORIGIN      
        1 catcataaat atcacagaca caaaccagac aaatatccta tcaatatgca acaaagtttt
       61 aattattcaa tttatagcag cacaaccaaa ctccccaaat aataaccaag agctattaat
      121 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      181 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      241 ccccgcttca ggtatactgt gcaccttaat tccacaaaca tcaggcagac catgacagac
      301 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      361 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
      421 tacaaagccg ttgaagccat gccaggtgtg atgcgcattc cagagaaaca tctggcacgc
      481 gaaattgaac gcatcgccaa cgccggtatt cgttccgtga tgactttcgg catctctcac
      541 cataccgatg aaaccggcag cgatgcctgg cgggaagatg gactggtggc gcgaatgtcg
      601 cgcatctgca agcagaccgt gccagaaatg atcgtcatgt cagacacctg cttctgcgaa
      661 tacacatctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
      721 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
      781 ccttctgccg cgatggacgg ccaggtacag gcgattcgcc aggcgctgga cgctgcgggc
      841 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggtccg
      901 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
      961 atgaaccgtc gtgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgt
     1021 ctgatggtta aacctgccgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1081 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1141 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1201 gcgggtgcgg atctgatttt cagctacttt gcgatggatt tggctgagaa gaagattctg
     1261 cgttaatttt gtttcctctc agaaaggggg agttaacctg ttgaaagtac ggtgaacgcc
     1321 ccctcaccct aaccctctcc ccaagggggc gaagggactg tccgtgctcg cttcatctat
     1381 atcaccgttc tttcgccgct cgaggtgacc gtccactctc gtatcacccc gcccgataaa
     1441 acggtttatc cccaagaatc gtcgcccgat gcataatccg tcgctgtggc aggtaatcgg
     1501 cattggcata gtgctgggtc acgcggttat cccaaatcgc aatatcattt ggttgccagc
     1561 gccagcgcac ctgaaactcc ggtttagtga tatgagcgaa taaaaaactt aacaaggctt
     1621 cgctctcttt ctcgctcaca tcaacaattc gcgtagtaaa gccttcattc acaaacagcg
     1681 cctgtttacc gctcaccgga tgcgttcgca ccaccggatg tagcaacggc ggatttttcg
     1741 cgaccgcctc gcgccagcgt tgatgttcct cctcggtttt gcggtatttg tattccggga
     1801 acgatttacg gaaatcatgc tccgcacgca gcccgctcag cagctggcgg aagggaacag
     1861 agagcgcctc ataggccgca ataccgctgg tccagagcgt atcaccgccg gtcgaaggta
     1921 actctttagc tgccagaatc gcccctgcgg gtggcgtttc aataaatgtc acatcggtat
     1981 gccagttgtc gttatccggc ggattatcgt tatgggtatc cagcacgatg atctcgtcaa
     2041 ccccttcggc atgagggtaa acagggtgaa tatgcagttc gccaaaacgc tgggccagcg
     2101 cgcgttgctg ctgcggcgta atagcttgat cgcgcagaaa taccacctga tggcgcagca
     2161 ccgcatggta aagctgttca aactgattat cgcttaacgg gcgcgtcagg tcggcacccg
     2221 aaatttgtgc gccgatatac ggccccagcg gggtaatgct caaacgttca ctcattgtac
     2281 ttctccatgc cagggcgtca ggcggcgctg taatgcgcgc agacccagtt ctaaaagaaa
     2341 ggcgataatc gcaatcaccg cgatccccgc cagcaccacg tcagtcgcga gaaattcacc
     2401 cgctgactga accataaatc ctaaaccgcg cgttgcggca atcagctccg ccgccaccaa
     2461 cgtagaccag cccacaccca gaccaatgcg taatccggtg aggatttccg gcagcgcgcc
     2521 gggcaaaatg acaaaccaca gcacctgcgc acggctggca cccagcgacc gggcggcacg
     2581 aatgcgaacc tgctgcgcgc ttttcacccc tgccagcgcc gacattgcca ccggcgcaaa
     2641 aatcgctaaa tagatcagta agatcttcga ggtttcgcca ataccaaacc aaatcaccat
     2701 cagcggcaaa taagccagcg gtggcaccgg acgataaagc tcgattatcg gatccagaat
     2761 gccgcgcacc gtcgggctta gccccatggc gatcccgacc ggaataccga ttaccaccgc
     2821 cgcgagtagc gccagcacaa tgcgcgtcag gctggcggcc agatgctgcc acagcgtggc
     2881 gtccataaat ccctgcggcc cggcaatggt gagcagtttt gccagtacct gttgcggtgg
     2941 cggcaaaaac agcgggctaa tcagttgcag cgctgcgact gcccaccata cagtgagcaa
     3001 aaccgctaac gtgccaatgc ttaaggtcac ctgacgcgag agcggccagc gccatttcag
     3061 ccgatgcgaa tgcagttttt cattaatgag cacactcatg agaacgcctc ccgctgctca
     3121 aatacgcggc ttaaaacata ttcgcgcatg gcgatgaatt gtggatcgga cttgatgctg
     3181 cggctcgact ctcccgcaac aaagcggcga gcaaagttga gcggcagccg ctccagcacg
     3241 cggccagggc cgggtgaaag cagaaccagt tcagtcgcca taaacaccgc ttcttctata
     3301 tcgtgggtaa tcaacagcac ctgcttgccc gtctcctgcc agagtttcag cagcagggtt
     3361 tgcatctggt cgcgggtgaa ggcatccagc gcaccaaacg gttcgtcgag taataacagc
     3421 tggggatttg ccgccagcgc gcgagcaatg cccacccgct gacgttgacc gccggagagc
     3481 tgccagatgt agcgtttttc tgctccttcc agccccactt ttttcagcat ctggtgcgcg
     3541 atttccagtc gctgcatttt ctctatacct gccagttgca ggccgaacgc cacgttgttc
     3601 tggacattgc gccacggtag cagcccttca ttctgaaaaa ccacgccgcg ctctgctccc
     3661 ggcccctcaa tacgcttacc cgccagttga atgctgccgt gctgataagg cacaaaaccg
     3721 gcaatcagat tcagcagggt ggttttaccg cagccggacg gccccagcac caccagtagc
     3781 tcaccgcttt ccagcgtcag gttgatatct tccagtgccg gtttgccacc ataattggcg
     3841 taaagatgag agatttgcag catctgcgcc tccttttatt gcacgaagcg cgaggtaacg
     3901 tactggctgt aatcattcgc tacagccggg accttgccct gctctttcaa aaactgcgcg
     3961 gtgtcgatga tcgctttgtt caccggtccg gtcagttctg ccgtttgttg ctgcggcgtc
     4021 agataggtat tccccttcac cagccccgga acgtcacctt caggcacgcc gcttaaccgc
     4081 gccagtttgc tgatgttttc cggctgtttc agccacgcgt ctgggttagc aatgtacggt
     4141 tgctgagcat cgatggcgct tttggcgaac gctttcacga cctcaggatg tttctcggca
     4201 aaatctttgc gcaccaccca gacgtccagt gtcggcgcgc cccactgccc gacctgttcc
     4261 gaatcggtca gcaccttgcc gtctttttcc agggcgttaa ccgccggtgc ccagacataa
     4321 gcaccatcaa tatctccccg ctgccatgcg gcgataatcg cgggcggctg caggttcaca
     4381 atctccactt gccctggttt aattccccag tgtttcagcg ccgccagcag gctgtagtgg
     4441 gtggtggaga taaacggcac ggcgatgcgt ttgccaatca gatcttccgg tttgctgata
     4501 gttttcttta ccaccagcgc ttcggagtta cccagttttg acgccagcaa gaagacttca
     4561 atcggcacct attggctggc tgcaaccgct aacgggctgg aaccgaggtt gccgatttgc
     4621 acgtcgcctg aagccagcgc ccgcacgatg ctggctccgc tgtcaaactt acgccagtcc
     4681 acgctcgccc cgctctcttt agcaaaggtg ttgtcggcct gagccacttt cgccggttcg
     4741 gctgaggttt gataggccac ggtgacgttc accgcctgtg cctgaaaagc gatgaatgcc
     4801 agtgcggcta gaagtgtgtt acgcgatgaa agtgccatga ttgtctgctc ccctgtcttg
     4861 ttatgggagc agtattcagg aataaaaaca ttcattaaaa gaattagtcg ttatcgcaca
     4921 gatgatttta ttcttagcaa aaaaacggca aaggaaagat taaatatgca attactccgt
     4981 tctaacaatt gggaaaatta ctacacttca ttttagtgat gcataaacaa ccacattacg
     5041 agatttatta cttcattatt tctatcaata tcaatacata taataaaact attatattca
     5101 cattgtatat atcactgtat cattctcaat cactcgcata ttacattcac ataatgtata
     5161 taaacaatgc ttgttcacag aacatatttt ttaaatgcaa ttaattgaca
//
LOCUS       DAZFZC010000200         5156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFZC010000200 DAZFZC010000000
VERSION     DAZFZC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.202"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..432
                     /locus_tag="ACHKXP_004976"
     CDS             <1..432
                     /locus_tag="ACHKXP_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7649779.1"
                     /translation="YEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVM
                     AGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSL
                     AWGAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            497..3604
                     /locus_tag="ACHKXP_004977"
     CDS             497..3604
                     /locus_tag="ACHKXP_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7649780.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSATSSAGTASTKATEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEASRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAANDNANGRVPSNRKVNG
                     KALTADITLTPKDIGTLNSVTISFSGGAGWFKLATVTMPQASSIVYIALIGGAGYNVG
                     SPQQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTYDIYVEIGNYAT
                     SVNIHWDCTTNASVSIYTSPTYSASKPSSVTGGVVYTMYSSHQKPTPSDIGALPTTGG
                     TISGPLSVTDGITGALKGNADTATKLAAAPKINGVKFDGSADINLTPENIGAFARRST
                     GAYADSDGAVPWNAESGAYNVTRSGNSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRS
                     SRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKL
                     AAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASVSSTDLGTKTTSSFD
                     YGTKSTNNTGAHTHSVSGTAASAGAHTHSMTFVSGGSSGAPGSGSPDYSKYSVNTSSA
                     GAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNY
                     IVRLA"
     gene            3604..4179
                     /locus_tag="ACHKXP_004978"
     CDS             3604..4179
                     /locus_tag="ACHKXP_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7649781.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQIASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(4277..4867)
                     /locus_tag="ACHKXP_004979"
     CDS             complement(4277..4867)
                     /locus_tag="ACHKXP_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7649782.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         1339 a         1255 c         1395 g         1167 t
ORIGIN      
        1 tatgagttta cggacacact ggggatggtg acgtcgttca gctatgcagg agacaagaat
       61 cgccagatta cccgttacag cgatacccgc tggcatgaag attccgttcg taaccgctgg
      121 ttcagcgtaa tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg
      181 gcgggtgtgg cttacagccg tgtgtcgact ttctccgggg attatctccg cgtaactgac
      241 aacaagggga aaaagcacga tgtgctgacc ggaagtgatg acggtcgcca cagcaacacg
      301 tctctggcgt ggggagctgg cgtgcagttt aacccgaccg aatccgtggc cattgatgtc
      361 gcttatgaag gctccggcag tggtgactgg cgcactgacg ggttcatcgt gggtgttggt
      421 tataaattct gattagccag gtaacacagt gttatgacag cccgccggtt caggcgggct
      481 tttttgtggg gtgaatatgg cagtaaaaat ttcaggtgta ctgaaagacg gcacaggaaa
      541 accggtacag aactgcacaa tccagctgaa agcaaaacgt aacagtacca cggtggtggt
      601 gaacacgctg gcatctgaaa atccggatga agccgggcgt tacagtatgg acgttgagta
      661 cggtcagtac agcgttattc tgttggtgga aggattcccg ccgtcacatg ccgggaccat
      721 cacagtgtat gaagattccc gacccggtac gctgaatgat tttctcggtg ccatgacgga
      781 ggatgatgcc cgtccggagg cactgcgccg ttttgagctg atggtggaag aggtggcgcg
      841 taacgcgtcc gtggtggcac agaacacggc agccgcgaag aagtcagcca gtgatgccag
      901 cacatcagcc cgtgaggcgg caacccatgc gactgatgct gcaggctcag cacgcgcagc
      961 cagcacgtca gccggacagg ccgcgtcgtc ggctcagtca gcgacttcca gcgcaggaac
     1021 ggcatcaaca aaggccactg aagcatcaaa aagtgctgcc gctgcagagt cctcaaaaag
     1081 cgcggcagct accagtgcca gtgccgcgaa aacgtcagaa acgaatgcgg cagcgtcaca
     1141 acaatcagca gccacttctg catccaccgc gactacgaaa gcgtcagaag ctgccacctc
     1201 agcccgggat gcggcggctt caaaagaggc ggcaaaatca tcagaaacga acgcatcctc
     1261 gagcgccagt agcgcagctt cctcggcaac agcggcagga aattccgcga aggcggcaaa
     1321 aacgtcagag acgaacgcca ggtcttctga aacggcagcg ggacagagcg cctcggctgc
     1381 ggcaggctca aaaacagcgg ctgcgtcgtc tgccagtgca gcgtcaacaa gtgccgggca
     1441 ggcctcagcc agtgccaccg ccgccggaaa atcggcagaa agcgccgcat cgtctgcttc
     1501 aacagccaca acgaaggctg gcgaagccac tgaacaggcc agcgcagcag cgaggtctgc
     1561 ttccgcagcg aagacatccg aaacgaacgc gaaagcgtcg gaaaccagcg cagaatcctc
     1621 aaaaacggct gccgcatcgt ccgccagttc ggcggcgtca tcggcatcat ctgcgtctgc
     1681 ttcaaaagat gaggcgagca gacaggcatc agcagcgaaa ggcagcgcca cgacggcatc
     1741 cacgaaggcg acagaggcag ctggtagtgc gacggcggca gctcagagca aaagtacggc
     1801 ggaatccgcg gcaacgcgcg ccgagacagc agctaaacgg gcagaggata ttgcatccgc
     1861 cgtggcgctt gaggatgcaa gtacgacgaa aaaggggata gtacagctca gcagtgcgac
     1921 caacagcaca tctgaaacgc ttgccgcgac accgaaggca gttaaagcag cgaatgacaa
     1981 tgcgaatggc agggtaccat ctaaccgaaa agttaacggg aaagcactga ctgcggatat
     2041 aacattaaca ccgaaagata ttggcacttt aaattcagta acgatctctt tctctggcgg
     2101 ggcagggtgg ttcaaactgg caacggttac catgccacaa gcgagttcca tcgtttacat
     2161 cgcattgatt ggtggcgctg gttacaacgt cggctcccca cagcaggcag gcatttcaga
     2221 actggttcta cgagcaggca atggaaaccc caaagggatt accggagctt tgtggaagcg
     2281 tacagccgtc ggattaacga atttcgcctg gatcaacaca tccggcgata catatgatat
     2341 ttacgttgag attggcaatt atgcgacgag tgtaaatatc cattgggatt gtactacaaa
     2401 tgcgtcagtt tctatttata cctcgccaac atattcagcg agtaagcctt ccagcgttac
     2461 cggtggtgtt gtttatacga tgtatagctc acatcagaaa cctacaccat cagatattgg
     2521 agcgctgcca acgactggag ggactatttc aggtccgttg tctgttactg atgggatcac
     2581 cggggcactg aaggggaacg ccgataccgc gacgaaactt gcggcagccc caaaaattaa
     2641 cggtgttaag tttgatggct cggcggatat taacctcacg ccggaaaata ttggtgcatt
     2701 tgcccgacgt tcgacggggg cttatgcgga ttcggatgga gccgttccct ggaatgccga
     2761 atcaggcgct tacaatgtca cccgctctgg caacagctat attctggtta acttctatac
     2821 cggagtcgga agttgccgga ccttgcagat gaaggcacat tacagaaatg gaggtctgtt
     2881 ctaccgttcc tcaagagatg gctatggttt tgaggaagac tgggcagaag tttatacctc
     2941 gaaaaatctt ccaccagaaa gctacccagt cggcgcacca atcccgtggc catcagatac
     3001 cgttccgtct ggttatgccc tgatgcaggg gcagactttt gacaaatctg cttacccgaa
     3061 acttgcagcc gcttatccgt caggtgtgat ccctgatatg cgtggctgga cgattaaggg
     3121 caaacctgcc agtggtcggg ccgtattgtc tcaggaacag gacggcatta aatcgcacac
     3181 ccacagcgcc agcgtatcca gtacggattt ggggacgaaa accacatcgt cgtttgatta
     3241 cggcactaaa tccacgaata acactggtgc gcatacccat agtgttagcg gtacggctgc
     3301 ttcagccggt gcacataccc attcgatgac atttgtttca ggtggttcca gtggtgctcc
     3361 gggaagtgga tcacctgatt attctaaata cagtgttaac acttcttctg caggcgctca
     3421 tacgcactct gtatcgggta ctgctgcaag cgcaggtgca cacgcacata ctgtcggtat
     3481 tggtgctcat acgcactccg ttgcgattgg ttcacatgga cataccatca ccgttaacgc
     3541 tgctggtaac gcggaaaaca ccgtcaaaaa catcgcattt aactatattg tgaggcttgc
     3601 gtaatggcat tcagaatgag tgaacaacca cggaccataa aaatttataa tctgctggcc
     3661 ggaactaatg aatttattgg tgaaggtgac gcatatattc cgcctcatac aggtctgcca
     3721 gcaaacagta ccgatattgc accgccagat attccggcag gcttcgtggc tgttttcaac
     3781 agtgatgagg catcgtggca tctcgttgaa gatcatcggg gtaaaacggt ttatgacgtg
     3841 gcttccggcg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa tgttacctgg
     3901 ttatcgccgg gaggggaata tcagaagtgg aacggcacag cctgggtgaa ggatacggaa
     3961 gcagaaaaac tgttccggat ccgggaggcg gaagaaacaa aaaacagcct gatgcagata
     4021 gccagtgagc atatagcgcc gcttcaggat gctgtggatc tggagatcgc aacggaggaa
     4081 gaaaccttgt tgctggaagc ctggaaaaag tatcgggtgt tgctgaaccg tgttgataca
     4141 tcaactgcac cggatattga atggccagca ctgccgtagg gtaaaacata taaattctat
     4201 aattagatgt atctctccat ttacggcaag gaagggggct tggaagacgt aaagcatctc
     4261 acaccgagat tattttttat atgtcaggtg tctgaagttt tgctttggct cttaaaatgg
     4321 tttgccgcga ggttttgaat tcccgggcaa tggcacttat acttacacct gacttaattc
     4381 gttcgaatac cacctgtttc tgttcttcat ttaacacagg tggtcgacca aaacgtttcc
     4441 ctgcgccgcg ggctcttact atcccggaat gagtgcgttc aagtaaaagg tctcgttcaa
     4501 attcagcgac tgctgaaatt acgtgcatca tcatttttcc tgttggactg gtcaggtcaa
     4561 tgccccccaa tgctaagcaa tgcactctga tacctgtttc ggtcagttgt tccactgttt
     4621 tcctgatatc cattgcatta caaccaaggc gatccagttt tgtcacaatc aattgatcac
     4681 cacatttcag gcgagcaagc aaccggttaa aaccaggacg ctcactggtt gctgctgagc
     4741 cgctaatgtg ttcttcgatt atttgctgag gtttgatttt aaaacctgca ctttcgattt
     4801 cccggcgttg attttcggtg gtctgatcca gcgttgatat ccgacagtaa gcaaaaattc
     4861 gagacatagt gagactctat acgaaattgg tgttcatatc ataatgcatc tcagaaaata
     4921 attttgatta tttttgtgca tatttgtatg tacacgttcg aaaataaacg aatgcgtatg
     4981 caaccccgta actttggtga gacccaaaat cgattttgtg aaaaatggct ttaactcggt
     5041 ttgtttttcg agttccgggc ggactcaagg aagaagaata gtgttgcgtg ttattttaac
     5101 cagatttcaa gttgtttggt cgtggaaaag tggagcaaaa tgttgttaaa gtggaa
//
LOCUS       DAZFZC010000201         5117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFZC010000201 DAZFZC010000000
VERSION     DAZFZC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.203"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(245..697)
                     /gene="yafP"
                     /locus_tag="ACHKXP_004980"
     CDS             complement(245..697)
                     /gene="yafP"
                     /locus_tag="ACHKXP_004980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7649783.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMISSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCVERYIDMLFVDPEYTRRGVACALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQY"
     gene            complement(707..1105)
                     /gene="yafO"
                     /locus_tag="ACHKXP_004981"
     CDS             complement(707..1105)
                     /gene="yafO"
                     /locus_tag="ACHKXP_004981"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO7649784.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKQARDNNQMHKIGKMAEAFRMRF"
     gene            complement(1108..1401)
                     /gene="yafN"
                     /locus_tag="ACHKXP_004982"
     CDS             complement(1108..1401)
                     /gene="yafN"
                     /locus_tag="ACHKXP_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO7649785.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE"
     gene            complement(1453..2508)
                     /gene="dinB"
                     /locus_tag="ACHKXP_004983"
     CDS             complement(1453..2508)
                     /gene="dinB"
                     /locus_tag="ACHKXP_004983"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO7649786.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2579..3364)
                     /gene="lafU"
                     /locus_tag="ACHKXP_004984"
     CDS             complement(2579..3364)
                     /gene="lafU"
                     /locus_tag="ACHKXP_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO7649787.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQSLVQKLDKQQVLSQRTR"
     gene            3336..5048
                     /locus_tag="ACHKXP_004985"
                     /pseudo
     CDS             3336..5048
                     /locus_tag="ACHKXP_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
BASE COUNT         1208 a         1340 c         1319 g         1250 t
ORIGIN      
        1 taccattcca tttatactcg tcatacttca agttgcatgt actgcgactc gaattattta
       61 gagtatataa atataaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat
      121 taatcctgga aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt
      181 gcagtttgtg gaataagaaa acgcgcacat accaggcggg cgttattttc attgcaagct
      241 ggatttaata ttgcggttta tatcgcatat aaaaattagt aaaccacgtt ccccggcatt
      301 caacgcgctg ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta
      361 tgctggcgtc caccgtaagt tcggattcag acttaatcaa aggttttaac aaagcgcagg
      421 caaccccgcg gcgggtgtat tcagggtcaa caaataacat atcgatataa cgttcaacgc
      481 aggtaataaa accaaccggt tgtgcattaa tgacggcaac ccgcacttgt gatttcgcga
      541 gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggtgaat
      601 aatgctgact ggagatcatc gtaaccgctc taatgaaaat agcgcatagt tgctgaaaat
      661 cgccaggctg atagtctctt atttgtatgt tattcataat ataaattcaa aaacgcatgc
      721 gaaacgcttc tgccattttc ccaattttat gcatttggtt gttatctcga gcctgtttat
      781 gaggttcagg tttcagaatg gcaatgagca accatgcttg ctcatcaaac gcaccctgac
      841 aatataccaa atgcgcttcg tcattcgttc tgctgaattg gcgcaactgt ggcggaaatg
      901 gattattcac atttgccaga tgaatatgag caactcgctc aaatttgatt aatggccagg
      961 taaacgagtc gtcgtagagt gcatcgcgac caaatatatc tggcaaaaca ccgtcacgct
     1021 tataggaaat aaaatccgcc gttaacgcat caagttcctc tgctgtaagt tgcaggcgaa
     1081 taagttttgt tttgaatacc cgcatcctta ttccttaaag tcattgaaat catcatccgt
     1141 catatcatta agatattttt cagctcggcg tgtaatttcc tctaatcttg cagcacttgg
     1201 acggaagcgc gcctttatgg gctttttctc ctcttgttca gcaagcatgt ccattaacgc
     1261 ttcgaatgcg ctggcactta agagatatcc tgcggggcga ttattagaaa gaaccgcaac
     1321 cggttgatca ataaagtatt tagctgggtt tttacgtaac tcagtaatat tgaccgattt
     1381 ttcagcgaga attcgatgca tgcagtgatc ccctcataat aatgcacacc agaatagaca
     1441 taatagtata catcataatc ccagcaccag ttgtctttcc atttgcggat caagcaacgt
     1501 cacatgcagc cccaccagtc gcacaccgcg cccgccgcgg cgttcatccc aggttttacg
     1561 cgcggtggcg attaagtcag ctttattcag ccgcggccag acgtgctcct gagtggtttg
     1621 ctgaaaatcg tcgaacttta atttcacccc ctggcgggca atcagtaaat caggttttac
     1681 ctttgccaga cggcgttcaa gttccggata cagccgctcg ataatcgctt cacattcaga
     1741 ccagtggtgg atatcttccg ccatcgtgcg ttccacgccg acggattttc gcaaccgttc
     1801 gctgttaacg tcgcgctcgt caatcccctg actacgctcc cacaaaatgc ggccaaattt
     1861 gccaaagcgt ttaagcagca tcaccagatc acacttttgt acatcaccgc aggttcgtag
     1921 ccccatcgct tccagttttg ccgccgagac tttgccgacg ccggggattt ttgccagtgg
     1981 taaggtttgt aaaaatgccg gaacttctgc cggcgtaatc acaaactggc cgttgggttt
     2041 attcatgtcg gaggcgattt tggcgagaaa ctttacgggt gccacgcccg cagacgccgt
     2101 cagttgcagc tcgttgaaaa tcgtctggcg gatttcctgg gcgatgaggg tcgcagaacc
     2161 gtggcaatgg acgctatcgg tgacgtcgag ataagcctca tccagtgaca acggttcaat
     2221 acgcgaggtg tagcgcgaga agatttcgcg gatatgattt gaggcttctt tgtaggcgtc
     2281 aaagcgcccc ggaagcaagg tgagatgcgg gcataatttg agcgccatcc ctgtcggcat
     2341 agcgctacgt acgccaaatt tacgcgcggg ataattggcg gtgctgatca ccccccgacg
     2401 ttcgcggctg ccgccaatag caatagggat atcgcgcagg gcgggattgt cgcgcatctc
     2461 cactgcggcg aaaaagcagt ccatatccac atgaatgatt ttacgcatta ctcacctctc
     2521 aacactggta aagtatacag tgatttcagg gtttgagaaa tgcgtaaaga ttcagcattt
     2581 agcgcgttcg ctgcgaaagc acctgctgct tatccagctt ttgcaccagc gactgcacca
     2641 ctttatcccc atgctgacca aagtattgat acagcgtatc ggacgcactt ttggtcagca
     2701 ccataatctc gatgcgccga ttacccgcac tttgcggatt cttcgcgtcc agcagcatct
     2761 ggtccgccat tgcgcttacc tgcatcactt tatcttccgg cattccggcc tcttccagca
     2821 cccgacgagc cgaaagcgcg cggtcacccg aaaggttcca gttgttgtag atattgtttt
     2881 tgtaggccat cgcatcggta tgcccggtaa taataatttt attatcgagc gagtcgaaca
     2941 ctggcgcgag ctccaccagc agcgttttaa agaacggcat aatcttcgcg ctgccgcgtt
     3001 caaacatatt gcggttctgg tcatctttaa tcagcacgcg caatccctgg ggaacaatct
     3061 ccatctccag attcgcttcc atatgcgcat cacgggcaat ggtgttgata ctggttgcca
     3121 gctcgccaag ctcggtggcg gatttcttct tcagcagcgc tgttttctcg ttgacgtctc
     3181 gcgctttttt ctccgtctct tccggcaccg ccacggtggc aggttttggc ggatgagacg
     3241 ggctgatttt atttaacggc gacaatccgc cgccactgaa aatcgactgc ccgtgcagcg
     3301 cggcgataat gctttcacgc tcggatttac tgacgctgtt gacaatcaac tttatcgtcg
     3361 tcaccaaagg ggccgagcgt atttccgagg tttctgcccg ctttaccctg gacgcgatgc
     3421 ccggtaagca gatggcgatt gatgccgatc ttaacgccgg attgatcaac caggcgcagg
     3481 cgcaatcccg gcgtaaagat gttgccagcg aggccgattt ctacggcgcg atggacgggg
     3541 catcgaagtt tgtgcgcgga gacgccatcg ccgggatgat gattctggcg atcaacctga
     3601 tcggcggcgt ctgtatcggc atcttcaaat acaacctgag cgccgacgcc gccttccagc
     3661 aatacgtgct gatgaccatc ggcgacggcc tggtggcgca gatcccttcc ctgctgctct
     3721 ccaccgcggc ggcgattatt gtcacccgcg tcagcgacaa cggcgatatt gcccatgacg
     3781 tgcgccacca gctgctggca agcccgtcgg tgctctacac cgctaccggg attatgtttg
     3841 tgctggcggt ggtgccgggg atgccgcacc tgccgttttt gctgttcagc gccctgcttg
     3901 gctttaccgg ctggcggatg agcaagcgcc cacaggcggc ggaggcggaa gagaaaagcc
     3961 tcgaaacgct gacccgcacc atcactgaaa ccagtgagca acaggtcagt tgggagacga
     4021 taccgctgat cgagcccatc agcttaagcc tgggctacaa gctggtagcg ctggtggata
     4081 aagcccaggg caacccgctc acccagcgaa ttcgcggcgt gcggcaggtg atttctgacg
     4141 gtaacggcgt gctgctgcct gagatccgca ttcgggaaaa cttccgcctc aagcccagcc
     4201 agtacgctat tttcatcaac ggcattaagg ctgatgaagc ggatattccg gcggataaac
     4261 tgatggccct gccctccagc gaaacctacg gcgagattga cggcgtgctg gggaacgacc
     4321 cggcgtacgg aatgccggtc acctggatcc agcccgcaca gaaagcgaag gcgctgaata
     4381 tggggtatca ggtgatcgac agcgccagcg tgattgccac gcatgtgaac aagattgtgc
     4441 gcatctatat tcctgatttg tttaactatg atgacatcac gcaactgcat aatcgtttgg
     4501 cgtcgatggc accacgcctg gcggaggatt taagcgcggc gctcaattac agccagttgc
     4561 tgaaagtgta ccgggcgctg ctgaccgaag gcgtttccct gcgcgatatc gtcaccatcg
     4621 ccaccgtgct ggtcgccagt agcgcggtga cgaaagatca tattctgctg gcggccgatg
     4681 tgcgcctggc gctgcggcgc agcattactc atccgttcgt tcgcaagcag gagctggcgg
     4741 tgtatacgct gaataatgag ctggaaaatc ttctgaccaa cgtggtgaat caggcgcaac
     4801 agggcggcaa agtgatgctc gacagcgtgc cggtggaccc gaatatgctt aaccagtttc
     4861 agagcacgat gccgcaggta aaagagcaga tgaaagcggc ggggaaagat ccggtgctgc
     4921 tggtgccgcc gcagctgcgc cctttgctgg cgcgttatgc aaggttgttt gcgccggggc
     4981 tgcatgtgct gtcgtataac gaagtgccgg atgagctgga gttgaagata atgggggcgt
     5041 tgatgtaaga atccggcacc agcgcacaac cgccggatgt ggcgtaaacg ccttatccgg
     5101 cctaccgatc cggcaca
//
LOCUS       DAZFZC010000202         5101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFZC010000202 DAZFZC010000000
VERSION     DAZFZC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.204"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..657
                     /locus_tag="ACHKXP_004986"
     CDS             <1..657
                     /locus_tag="ACHKXP_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO7649788.1"
                     /translation="YNKEDDFLYATQNSPAQLLKIARSGDIIGRAPLPFISDAETIEH
                     IQGNIFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFEGLAWKAEGRMLF
                     VAKERRPTGMFAYQLSPDLLSAKQVTIPEELNDIRVNDISGLDFNNESLMILSDESRK
                     LLKFNLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVTLPDESIYVASEPDILAKFVPNK
                     "
     gene            863..1738
                     /locus_tag="ACHKXP_004987"
     CDS             863..1738
                     /locus_tag="ACHKXP_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO7649789.1"
                     /translation="MFSIRSQLPISASVSIPAEQSQSISTIPGGRKIETVPEKEGLLV
                     FLGTKSVGEYALNILGQNIPRTMTGKKTYDILFLNETTKNDFDKKKTEFTFPGANRSH
                     LQSSNTGVAVDAGINFTVMEMKSMLPKDLTPGRYDKIYLIGDGSAGLPLLKCGDEFLS
                     PTDIVNRLVEHNLHEVEDIRLTSCHSANITKNNDFSPEEIEKSTKTNSGWLASALFGQ
                     KRSLAEHVYAEFEGRGINVSVSGYHGTGVFYVPEHGKPTTHLRSTTVPATPEQTVRRS
                     DYRVSLGRTQPTDID"
     gene            complement(1978..2250)
                     /gene="yjdJ"
                     /locus_tag="ACHKXP_004988"
     CDS             complement(1978..2250)
                     /gene="yjdJ"
                     /locus_tag="ACHKXP_004988"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7649790.1"
                     /translation="MEIHEGHNKFYINDAQGKQIAEIVFVPTGEHLAIIEHTDVDESL
                     KGQGIGKRLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(2262..2492)
                     /gene="yjdI"
                     /locus_tag="ACHKXP_004989"
     CDS             complement(2262..2492)
                     /gene="yjdI"
                     /locus_tag="ACHKXP_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO7649791.1"
                     /translation="MDQELLDAGYRVYTGEKIDVYFNTTMCQHSGNCVRGNSKLFNLK
                     RKPWIMPDTVDVDIVVKVIDTCPSGALKYRHK"
     gene            2660..4291
                     /gene="dcuS"
                     /locus_tag="ACHKXP_004990"
     CDS             2660..4291
                     /gene="dcuS"
                     /locus_tag="ACHKXP_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO7649792.1"
                     /translation="MRHSLPYRMLHKRPMKLSTTAILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTN
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPVYDENNKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSILFGALVGGLGTWILVKVLKRILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYHKSQDDEKLSTLSQAWSQV
                     VDVSEVLRDGTPRRDEEITVKDRLLLINTVPVRSNGVIIGAISSFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLLLNSESQLPDSGSEDQVTTLITVLGNLIENALEAL
                     GSQPGGEISVTLHYRHGWLHCEVNDDGPGIAPEKIDRIFDKGISTKGSERGVGLALVK
                     QQVESLGGSIAVESEPGIFTQFFVQLPWDGERSNR"
     gene            4288..5007
                     /gene="dcuR"
                     /locus_tag="ACHKXP_004991"
     CDS             4288..5007
                     /gene="dcuR"
                     /locus_tag="ACHKXP_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO7649793.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSGTPVDLILLDIYMQKENGLDLLPALHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSANEQDTRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT         1510 a         1177 c         1091 g         1323 t
ORIGIN      
        1 tataataaag aggatgattt cctgtatgcc acccagaata gccctgcgca gctacttaaa
       61 atagccagaa gtggcgacat catcggccga gccccgctgc cttttatctc tgatgccgaa
      121 accattgagc acatacaagg gaacattttt gctgcagtag acgaaaagac ttctgaacta
      181 ttctttttta cagttacaaa agatatgcat atctccttgc gaaataaaat tcagttagaa
      241 aaattcaata aaaaaaatag agggtttgaa ggactggcat ggaaagcaga aggccggatg
      301 ctgtttgtcg ctaaagaacg acgccctaca ggcatgtttg cctatcagct ttctccagat
      361 ctgctgagtg caaaacaagt aacgattcca gaggaactaa atgatatacg tgttaacgat
      421 atatccggcc tggactttaa taatgaaagt ctgatgattc tgtccgatga atcgcgtaag
      481 ttactcaagt ttaatctgac agaaatgtct ttcgttgaga tgatggacct gacaaaagga
      541 aatcattctc tgaccagtga tttgctccaa cctgaaggca ttgtaactct gcctgatgaa
      601 agcatttatg tcgctagtga acctgatatc ctcgctaaat ttgtccctaa taaataagtc
      661 tgtcactagc cttagtattg agcgaccaaa tctggcaatc tcatgaaact cgcaaaatac
      721 gaatctaata ttaataaaaa ttttactctg aaataaacag attaatagct gaaaaggata
      781 tcagctatta tcaactaaaa tattactcac tccattagaa tcatcatgaa ctcacattta
      841 aacaaactaa gaaggaggtt gtatgtttag tataagatcc cagctaccta taagcgccag
      901 cgtatcaatt ccggcagaac aatctcaatc catctccaca attcctggag ggagaaaaat
      961 agaaacagtg ccagagaaag aaggattgct tgtattttta ggaacaaaat cagtaggtga
     1021 atatgctctt aatattcttg gtcaaaatat tccaagaacc atgacaggaa aaaaaacata
     1081 tgacatatta tttctgaacg aaacgacaaa aaacgatttt gataaaaaga aaacagagtt
     1141 tacctttcct ggtgcaaaca gaagtcatct acaatcaagc aacactggtg ttgctgttga
     1201 cgcgggtata aattttaccg ttatggagat gaaaagtatg ctaccaaagg atttaacacc
     1261 aggaagatat gacaaaattt atctgattgg ggatggttct gctggcctac ctcttcttaa
     1321 gtgtggagat gaatttttat caccaactga tatcgtcaat cgtctggtag aacataatct
     1381 tcatgaagta gaagacatca gattaacatc ttgtcactca gccaacataa caaaaaacaa
     1441 tgatttctct cctgaagaaa tagaaaaatc aacaaaaacc aacagcggct ggttggctag
     1501 tgcattattt ggtcaaaagc gttcattagc agaacacgtc tatgccgagt ttgaaggccg
     1561 tggaattaac gtttctgtat ccggttatca tggcactggt gttttttatg ttccagagca
     1621 tggcaaacca acaacgcatc tacgctccac aacagtgcct gcaacacctg agcagacggt
     1681 aagaagaagc gactacagag tctcccttgg cagaactcaa cccacagata ttgactaatt
     1741 gagtctgcat ataggtttgt gtcattacct gatgcgctac gcttatcaga cctatattag
     1801 atcctgcaat atgtttaatt tgtaggcaga atagagcgtt cacaccacat caggtataaa
     1861 caaagtacag cttgccaaaa acctgaataa ccccgaattt acggggttat tcagcttaaa
     1921 catgcccata acatcacttc tgatttgtat tgcttacgac ggatacaatt tctctgctca
     1981 gctacgaata tcatcatact cgcgcgtttt atcgaactcg tgtttcgcaa acggacatag
     2041 cgggataatc ttccgcttct cccggcgcat tttttccacc accttcgcca ccagccgttt
     2101 accaatcccc tgccctttca gactttcatc aacatcggta tgttcgataa tggctaaatg
     2161 ttctcctgtc ggcacaaaaa caatttcagc aatttgtttt ccctgtgcat cattaatata
     2221 aaatttatta tgcccttcat gtatttccat cattccctca cttatttatg gcgatatttc
     2281 agtgcgccac ttggacaggt atcaataacc tttaccacaa tatcgacatc gactgtatca
     2341 ggcataatcc acggcttacg ttttaaatta aatagcttgc tattaccacg tacacaattg
     2401 cctgaatgct gacacatcgt agtgttaaag tagacatcaa ttttctcgcc ggtatacacg
     2461 cgataaccag cgtccaacaa ttcttgatcc ataaactggc ctcccacatg ggtgtgattt
     2521 tcgctataag catagccccg taatcgtcag acaacacgtg ttgtttcagt tttcagacaa
     2581 aagccaaccc tcttactccc tttgaattac ctacttcatc agggataatg cttaggaaat
     2641 ttgtcacaca aggaagctga tgagacattc actgccctac cgtatgttac acaaacgccc
     2701 gatgaaactc agcaccacgg ctatcttaat ggtcagcgct gttttgttct cggtgctgct
     2761 agtggtccat ctgatttatt tctcacaaat cagtgatatg acgcgtgacg ggctggccaa
     2821 caaagcgctg gcggtggcgc gaacccttgc cgactcgccg gaaattcgtc agggcttgca
     2881 gaaaaaaccg caggagagcg gtatccaggc catcgctgaa gccgtacgca agcgtaacga
     2941 tctgttgttt atcgtcgtta ccaatatgca aagccttcgc tactcgcacc ccgaagcgca
     3001 acgcattggt cagcctttta aaggggacga tatcctcaag gcgctcaatg gtgaggaaaa
     3061 tgtcgccatt aatcgcggtt ttttggcgca ggccctacgt gtatttaccc ccgtctacga
     3121 tgaaaataat aagcaaattg gcgtagtggc gattggtctg gagttaagcc gcgtgaccca
     3181 gcagatcaac gacagccgct ggagcattat ctggtcaatt ttattcggcg cactggtcgg
     3241 cgggctggga acatggatcc tggtcaaagt gcttaagcgc atcctgttcg gcctggaacc
     3301 ctatgaaatc tctaccctgt ttgagcaacg ccaggcaatg ttgcaatcga tcaaagaagg
     3361 cgtcgttgcc gtggacgatc gcggtgaagt cacactcatc aatgatgccg cacaggaact
     3421 gcttaactac cacaaatctc aggatgacga aaagctgtcg acactaagcc aggcatggtc
     3481 gcaggtagtg gatgtctccg aagtattacg cgacggcacg ccgcgccgtg acgaagagat
     3541 taccgttaaa gatcgactat tgctgatcaa caccgtccca gtacgcagta atggcgtcat
     3601 catcggggct atctcttcct tccgggataa aacggaagtt cgtaagctga tgcaacgcct
     3661 ggatggtctg gtcaactatg ctgacgcact tcgtgaacgc tcccacgaat ttatgaataa
     3721 attgcacgtg attctcggat tattgcatct gaagagttat aagcagttgg aagattacat
     3781 cctcaaaacg gccaacaact atcaggagga gattggctct ctgctgggta aaatcaaatc
     3841 gccagtcatc gccggtttct taatcagtaa aattaaccga gcaacggatt tgggtcatac
     3901 gcttttgtta aacagcgaaa gccagttacc agacagcggc agcgaggatc aggttacaac
     3961 attaatcacc gtgttgggca atctgataga aaacgccctg gaagcgttag ggtcacaacc
     4021 ggggggcgaa atcagcgtaa cattgcacta ccgtcacggc tggctgcatt gtgaagttaa
     4081 cgatgacggt ccagggatcg cgcctgaaaa aatcgatcgc atttttgaca aaggcatctc
     4141 gacaaaagga agcgagcgag gcgtcggttt agctcttgtc aaacaacagg ttgaaagtct
     4201 tggcggcagc attgccgtag aatcggaacc cgggattttc acacaatttt ttgtccagtt
     4261 accctgggat ggggagaggt cgaacagatg atcaatgtat taattatcga tgacgacgca
     4321 atggtggcgg agttgaaccg acgctatgtc gcacagattc caggctttca gtgctgcggc
     4381 accgcctcaa cgctggaaaa agcaaaagag attatcttta acagcggcac gcctgtcgac
     4441 ctgatattgc tcgatatcta tatgcaaaaa gagaacgggc tcgatttact gccagccctg
     4501 cacaacgccc gttgtaaaag cgatgtgatt gtcatctctt ccgctgctga tgccgcaacc
     4561 ataaaagatt cgctgcacta cggcgttgtg gattacctga tcaaaccgtt ccaggcctct
     4621 cgctttgaag aggcgctaac cggttggcga caaaagaaaa tggcgctgga gaaacatcaa
     4681 tattacgacc aggccgagtt ggatcagttg atccacggca gcagcgccaa cgagcaggat
     4741 acccgccgcc tgcctaaagg attaacgccg cagactctac gtacgttgtg ccagtggatc
     4801 gacgcccatc aggactacga gttctcaact gatgaactgg ccaacgaagt taacatctca
     4861 cgcgtttctt gtcgcaagta tctgatttgg ctggttaact gtcacatttt atttaccagc
     4921 attcattatg gcgtcacagg ccgcccggtt taccgttacc gtattcaggc agagcattac
     4981 tcactactaa aacaatattg ccaataaact attattggtt atctgtaagt aacaattatt
     5041 acagtaatgc aaaatagcac tggcacaagt tacattgttc atcgataaaa tttgtgccag
     5101 a
//
LOCUS       DAZFZC010000203         5086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFZC010000203 DAZFZC010000000
VERSION     DAZFZC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.205"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..1990
                     /gene="mngA"
                     /locus_tag="ACHKXP_004992"
     CDS             74..1990
                     /gene="mngA"
                     /locus_tag="ACHKXP_004992"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO7649794.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHVLTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTFKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGADIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            2008..4641
                     /gene="mngB"
                     /locus_tag="ACHKXP_004993"
     CDS             2008..4641
                     /gene="mngB"
                     /locus_tag="ACHKXP_004993"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO7649795.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDVMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            4666..4893
                     /locus_tag="ACHKXP_004994"
     CDS             4666..4893
                     /locus_tag="ACHKXP_004994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649796.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
BASE COUNT         1259 a         1180 c         1373 g         1274 t
ORIGIN      
        1 aattgatcac ataatggtat tgttttatcg ggcacgctgg cgcgactata aaaacgatca
       61 agtgaggatc atgatgaatc tgacgactct gacccaccgc gatgcgttgt gtctgaatgc
      121 gcgctttacc agccgtgaag aggccatcca cgtgttgact caacgtcttg ctgctctggg
      181 gaaaatttcc agtactgagc aatttctgga agaagtgtat cgccgtgaaa gccttggccc
      241 gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc
      301 ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg atggcccgga
      361 agcagttgat ttagtggtgc tgctggcgat tccccccaat gaagcgggta ctacgcatat
      421 gcaactgctg acagcgctga ccacgcgcct tgcggatgat gagattcggg cgcgtataca
      481 gtcggcgacg acgcctgatg agttgctctc ggcgctggat gacaagggag gcacgcaacc
      541 ttctgcctct ttttccaacg cgccaactat cgtctgcgta acggcctgtc cggcgggtat
      601 tgctcacacc tatatggctg cggaatatct ggaaaaagcc ggacgcaaac tcggcgtaaa
      661 tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg cggatcaact
      721 caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt
      781 taatggcatt cccgcgcttt cagtgcctgt tgccgagccg attcgccatg cagaagcgtt
      841 gatccaacaa gcgcttacct tcaagcgtag cgatgagacg cgtaccgtac agcaagatac
      901 gcaaccggtg aaaagtgtca aaacggagct gaaacaggca ctgttgagcg gaatctcttt
      961 tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat tactgtcgca
     1021 aatcttcggg ctacaagatc tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa
     1081 gctgggcggc gggctgctcg gaattttgat ggtaccggtg ctcgcggcct ataccgccta
     1141 ttctctggca gataaaccgg cgttagcgcc aggctttgcg gctggacttg ccgccaacat
     1201 gatcggctcc gggtttctcg gcgcggtcgt tggcggattg atagccggtt acttgatgcg
     1261 ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga ctttttatct
     1321 ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg tgggggaacc
     1381 tgtcgcctgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc
     1441 gctgttgctg ggtgccattc tcggttttat gtgttccttt gaccttggag ggccagtgaa
     1501 taaagccgct tatgcattct gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc
     1561 cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca cgatgctcgc
     1621 accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc tgttagggct
     1681 ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc gggttattgg
     1741 ttcgtttgtg ctgggctcta tggtaacggg cgctattgtc ggtgcgatga atatcggcct
     1801 ttcgacaccc ggtgccgaca ttttctcgct ctttttactt catgataatg gcgcgggcgg
     1861 tgttatggcg gcaattggct ggtttggcgc ggcattggtg ggggctgcaa tctcgactgc
     1921 aattctcctg atgtggcggc gtcacgcggt taagcatggc aactatctga ctgatggcgt
     1981 aatgccataa acaaaaagga aacgacgatg aaagcagtat ctcgcgttca catcaccccg
     2041 catatgcact gggatcgaga gtggtatttc accaccgaag agtcacgtat tctgctggtc
     2101 aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa atattacgta
     2161 ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac
     2221 cgtgtgaaga aacaggtaga agccggcaag ttgattatcg gcccctggta tacccagacc
     2281 gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac
     2341 tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac cagattcctt tggcatgtcc
     2401 gggcaactgc cgcatatcta caatggattt ggcattaccc gcaccatgtt ctggcgcgga
     2461 tgttcggagc gccacggtac tgataaaacc gagtttttgt ggcaaagcag tgacggtagc
     2521 gaagtgacgg cgcaggtgct gccgctgggc tacgccatcg gtaagtactt acctgccgac
     2581 gaaaacggat tacgtaaacg cctcgacagt tattttgacg tgctggaaaa agcgtctgta
     2641 accaaagaga ttttgctgcc gaatgggcat gaccagatgc cattgcagca aaatatcttc
     2701 gaagtgatgg ataagctacg tgagatctac cctcaacgta agtttgtgat gagccgcttt
     2761 gaagaggtat ttgagaagat cgaagcgcag cgagataatc tggcaaccct gaaaggggaa
     2821 tttattgatg gcaaatatat gcgagtgcat cgcaccatcg gttctacgcg tatggatatc
     2881 aaaattgccc acgcgcgtat tgaaaataag attgttaatc tgctggaacc gctggcaaca
     2941 ctggcctgga cgttgggttt tgaataccac cacggcttgc tggagaaaat gtggaaagag
     3001 atcttaaaaa atcatgccca cgacagtatc ggctgctgct gtagtgacaa agttcatcgc
     3061 gaaatcgtcg cccgcttcga actggctgaa gacatggcgg ataatctgat tcgtttctac
     3121 atgcgcaaaa ttgccgacaa catgccgcag agcgacgccg acaaactcgt tctgtttaac
     3181 ctgatgccct ggccgcgtga agaagttatc aacaccactg tgcggctgcg cgccagccag
     3241 tttaatttgc gggacgatcg cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag
     3301 atcgatccag gcctaatcga tcggcaaata gttcattacg gtaattacga tccctttatg
     3361 gagtttgata tacagatcaa ccagattgtc ccttctatgg gctatcgcac gctttatatc
     3421 gaagcgaatc agcctggcaa cgtaattgcg gcaaaaagtg acgctgaagg gatactggaa
     3481 aatgctttct ggcaaattgc gctcaatgag gatggttctc tgcaactggt agataaagac
     3541 agcggtgtgc gctatgaccg ggtattgcaa attgaagaaa gctctgatga tggtgatgaa
     3601 tatgactatt cacccgcaaa agaagagtgg gtaattaccg cagcgaacgc gaaaccgcaa
     3661 tgcgatatta ttcatgaagc ctggcagagc agggctgtta tccgctatga catggcagtg
     3721 ccgctcaatt tgtcagaacg cagcgcccgg caatccactg gcagagtagg ggtggtgttg
     3781 gttgtcactc ttagtcataa cagcaggcgt attgatgtgg atatcaatct tgataaccag
     3841 gctgacgatc atcgccttcg tgtcctggtc cctacacctt ttaacaccga cagtgttctg
     3901 gcagatacgc agtttggttc gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg
     3961 cagcaagaag gctggaaaga agcgccggtt ccggtatgga atatgctcaa ctatgttgcc
     4021 ttacaggaag ggcgtaacgg catggctgtc tttagcgaag ggttacgtga atttgaagtc
     4081 atcggtgaag agaagaaaac ctttgccatt acgttgctgc gtggcgtggg cttactgggc
     4141 aaagaagatc tgcttttaag gcctgggcgg ccttcgggaa ttaaaatgcc agtcccggac
     4201 tcacaactac gtggtctgct ttcttgtcgc ctaagtttat tgagttatac cggtacgcca
     4261 accgccgctg gtgtagctca gcaggcgcga gcatggctga ctccagtaca gtgttacaac
     4321 aaaatcccat gggatgtgat gaagctcaac aaagccggat tcaacgtgcc ggaaagttat
     4381 agtttgttga aaatgccccc agtgggatgc ctgataagcg cacttaagaa agctgaagac
     4441 cgacaagaag tgattttacg gctgtttaat ccggctgaat cagcaacctg tgatgcgact
     4501 gttgctttca gtcgcgaggt gatttcttgc tcagaaacga tgatggatga acacattacc
     4561 accgaggaaa atcaaggttc aaatctatcg gggccttttt tacccggcca gtcacggacg
     4621 ttcagttacc ggcttgcctg aatagcaatc aaaccgaagc cacatatgcg cggccagatt
     4681 gttgacaaag ggcgctttgt tcatgccgga tacggcatga acgctttatt cggtctacaa
     4741 aagcaggcaa attcaatata ttgcagagat gatgtagaca ctggcaagcg tagcgcatca
     4801 ggtaattttg cgtttatctt cactctcaag ccacgtatat gtggctttat ttttaacaaa
     4861 ataataaccc tgggtgagtt aattataata taattataag ttaactaaat gttaatattg
     4921 gcggggtgga tttatgcctt tattagtaat cctgaaactc tgcgtcgtat tagccagtga
     4981 ccaaaaaaag aattaaggtc aaccgtgctg tttttgcttc gtctcttttt atctttaatt
     5041 gccaaccgaa actaatttca gccttataac tcacacattt taaaca
//
LOCUS       DAZFZC010000204         4964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFZC010000204 DAZFZC010000000
VERSION     DAZFZC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.206"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..397
                     /locus_tag="ACHKXP_004995"
     CDS             53..397
                     /locus_tag="ACHKXP_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO7649797.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            448..981
                     /locus_tag="ACHKXP_004996"
     CDS             448..981
                     /locus_tag="ACHKXP_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7649798.1"
                     /translation="MNTKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1255..1950
                     /locus_tag="ACHKXP_004997"
     CDS             1255..1950
                     /locus_tag="ACHKXP_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFO7649799.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            2045..2176
                     /locus_tag="ACHKXP_004998"
     CDS             2045..2176
                     /locus_tag="ACHKXP_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649800.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            2399..2584
                     /locus_tag="ACHKXP_004999"
     CDS             2399..2584
                     /locus_tag="ACHKXP_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7649801.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            2985..3311
                     /locus_tag="ACHKXP_005000"
     CDS             2985..3311
                     /locus_tag="ACHKXP_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO7649802.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(3443..3643)
                     /locus_tag="ACHKXP_005001"
     CDS             complement(3443..3643)
                     /locus_tag="ACHKXP_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7649803.1"
                     /translation="MSSKNRPRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3685..4050
                     /locus_tag="ACHKXP_005002"
     CDS             3685..4050
                     /locus_tag="ACHKXP_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7649804.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            4340..4903
                     /locus_tag="ACHKXP_005003"
     CDS             4340..4903
                     /locus_tag="ACHKXP_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7649805.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNSEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT         1244 a         1092 c         1417 g         1211 t
ORIGIN      
        1 tatttcaaaa tcagggagga ccgccgtaag acggcgcggg gagactaaag cgatgaagaa
       61 aaaatacgaa ctggttgtta aagagataaa taattacccg gataagattg ctgttactgt
      121 ggcacttgaa attggcgggc atccgtcgtt gttgttgcca gatgtggcga ttagtcttga
      181 ccgtactgaa ggagccacgc tggagtttta cgaagctgag gcgaaaaagc aggcgaagca
      241 gtttttcatg gatgttgctg ccgggttatg tgaaggggat ggtccgttgc cggaaaagcg
      301 gcccatcatt ttagaggcgc aggatgtgtt gataacctac agaggaaaac taccgggaat
      361 aattactggt tctctgaaga ctccaccgct ggcctgaaga cttaacatat ccagggattt
      421 gaaatcgata aaccctgata aatatccatg aacaccaaaa tcagatacgg cctgtcggct
      481 gccgttctgg cgctgattgc cgcaggtgcg cctgcgcctg aaatcctcga ccagtttctg
      541 gatgaaaagg aaggtaacca caccacggca taccgtgatg gcgcgggtat ctggaccatc
      601 tgccgcggtg ccatcctggt ggatggtaaa cctgtcgttc cgggcatgaa gttgtcgaag
      661 gaaaaatgcg accgggttaa cgccattgag cgtgataagg cgctggcatg ggtggcgaaa
      721 aacatcagag tgccactgac cgaaccccag aaagcgggga ttgcgtcatt ctgtccgtac
      781 aacattggcc ccggtaagtg tttcccgtcg acgttttaca gacggattaa tgctggtgac
      841 cgcaggggag catgcgaggc gattcgctgg tggattaagg acggtggcag agactgccgt
      901 attcgctcaa ataactgtta tggtcaggta tcccgtcgtg accaggagag cgctctggcg
      961 tgctggggaa tcgacagata agcagaatat tttgctgaaa aatgacgttg gccagcgcgg
     1021 gcggataaca cgaaatcctg cgaactggca aaatgtaagt gaataaagtt aggcagatta
     1081 tttcacgcag aggcaccgta atggtgcctt tgtcatttct gcgcttcgca caagcgtaaa
     1141 taaaccaaag aacctttcag gatgagccct ggtggataac cggcagtggt ctggttaacc
     1201 ctctttgggc tggttattcc tgtgcgcagg gttcatcact aaaaggaatc aaccatgaaa
     1261 gagatgattt ctgtcgatca tgaaatatcc atgagtagtc tggattttct gaataacatt
     1321 attaatccag cccgggcaga agccggagaa gtccctcatg aaccgcgtaa gtttcttgca
     1381 aaaattgagg atgagctaga gcttgatgga accggaaaaa aattccggtt aaacaataac
     1441 cagacaagaa cggcatacta tgatctggat tttgaccaga tgatgctcgt tggcatgagg
     1501 gagtcaaagg ccgttcgtcg ttctgtgctg gcaagactca aagcgatgca tggtattcag
     1561 atcccccgga ctttacctga ggcgttgcga tttgcggcaa aactggctga acagaaagca
     1621 gtgctggaaa atcaactggc aatagcagcg ccgaaggctg aatttgttga taactatgtt
     1681 gaagcatctg gtctgatggg attccgggaa gttgctaagt tactcggtat caaagaaacc
     1741 gatttccggc tgtttttgtt ggagaacgga ataatgtatc gcctcgctgg aaaaatgacg
     1801 ccttactcgc atcacctgga tgcggggcgg tttagcgtga aaacgggcga ggcgggcaat
     1861 ggtcatgctt ttacgcaggt taaattcacc ccaaaaggtg ttcagtggat tgctggtctg
     1921 cttgctgcat ggagagctac cgcagcatga agatgataaa aacggactgg aaatttttgc
     1981 tggtctggct gattccgttt ttatgggtgg ttgcccggtt aattactgct attaaggggt
     2041 aaagatgtca gacaaactca taatgctggc gaagggcctt tgtgtaatcg tcggtatttc
     2101 attttcacta atgctggttg ttctttttct ttccataacc tggatggcgt tgacttcggc
     2161 agggctggtg gggtgagcat aaaccgaatg ctttccgcgt ttaccgttat tctgctggtg
     2221 gtctgtggtg cgcttagtct ggggctgaat cattaccgcg ataacgtcat cacctacaaa
     2281 gcgcagcgcg ataaaaaagc cagagagctg gagctggcaa acgcaaccat tactgatatg
     2341 caggtgcgcc agcgcgatgt tgctgcgctc gatgcaaaat actcgaggga attagccgat
     2401 gcgagagctg aaaatgaaac tctgcgtgct gatgttgccg ctggtcgtaa gcgcctgcgg
     2461 atcaacgcca cctgctccgg taccgtgcgt gaagccaccg gcacctccgg cgtggataat
     2521 gcaaccggcc cccgactggc agacaccgct gaacgggatt atttcatcct cagagaacgg
     2581 ttgatgacaa tgcagaagca gctggaaggg gcacaggact atatccgcac tcagtgcctg
     2641 aactaagttt tgctgatgcg ccgtatcgtc gctgtattcc ctcattaaca gagaccgcag
     2701 cccgacaggg agactcctct gcgcgagtgt gcggggataa tcaaaaacga tacacaccgg
     2761 ggtttaccgc gttaacggag cgcggcgttg tcccctcatg gtcgctggtt cggtgcgatg
     2821 gtggaagaaa ccggacgatg tgttacctcg caagacctgt tatgtcatgt gtctgatttg
     2881 tgatttaagt cggataattg tcgttgccat taagcagagg attgatggcc gacagggtgg
     2941 cattgttaga ataagactta ttcttatctg tgccgggaat gaaaatgaaa agaaatcttc
     3001 cgttaattat tttgttgtct tctctggtta tgggctgtac gcaacataaa acagatatgc
     3061 cccgacagtt ggttaaggca ttaccacaat atccggccta tgcagcggca aattatataa
     3121 agggacgggt tgatgtgaag tttgatattg gtgctgatgg tactgtcacc cgaattgagt
     3181 ttatccgttc agagccgcac catctgtttg atgagcaggt tgtaaaggcg atggcaaaat
     3241 ggcgatttga gaaggacagg ccgcgtaaag gcgtgaagaa aacgtttatc tttagtcctt
     3301 ctgcaccctg attatttcat cagaaattaa ttatcactct gttgttattc tgtacattcc
     3361 ggctgggtaa gtcttgttcc gccgggtatg aagatgaaat attgttggag gacagtgggt
     3421 acccgctcct gtaaccgaac gttcatttct cgttatttgt catgctggcc ggacgcagat
     3481 gcgttgcatc tgttgccagc cttctcctgc aggcttcaat aacccacgct gaaaagttac
     3541 cggaaccttt atgttcaagg gcgatattga tctgttcaat catgtgattg gggaaacgga
     3601 tgttgcgggt tgttgttctg cggggccggt ttttcgatga cattttcttt cctctggtga
     3661 caagctatat ggcgaggatt ttacatggct gtgcttcgta cgttaccggg cagaatcaaa
     3721 actctgaaca cccggcgggt gaatattctg aagggtgaac agcgtcgtgt cagtggcagt
     3781 gcacgtgttt ccctcaagcg tcatatctgg ctcagggatg ccgggcagtg ctgtctctgt
     3841 ggtcgtgtgg ttgacctctg tgacagtgaa ctcgatcacc gaattgcact tcagttcggt
     3901 ggtggtaatg aggagacgaa tctctggacg ctctgtaccg aatgccatcg acaaaagtct
     3961 gctcgtgaag cggcgggtgg tatgccggac ccgacgctgc cggaggtgtc cggaggtagt
     4021 ggcagagcgg acgacatcat cggactgtaa cccgagcggg gggggtatca tccggcgtaa
     4081 aaaacgatcg ctccggacac cgccccccct ctcacgcaga gaaaaaattc ccgtttcagg
     4141 gcagttaaca tgttaactgg ctgtccgggc atttttgcgg tttttatctt tattattcag
     4201 tttgttgtgc ggaaaaaatg ttaactggct ttttcagcaa atgttaacca ggcagcagtt
     4261 aacatttgcg gcatgagacg ccgggaaaaa tgggctgaac catacccggc tgagtgcgtt
     4321 ctggacccgg gaggaggctg tgctgacaac gcaaaaacga aaatttgcgc tggcgctcat
     4381 gtccgggaaa aacaaaacag cgtcagccat tgccgctggt tattcggcga agaccgccag
     4441 ggttaaaggc tcgcagctgg caaaagatcc ggaggtgctt gcgtttatag cccgtaaaca
     4501 atgcgagacg gtggaggtgg atgaggttcc tgtttaccgg cagaaaaaat cagagcagga
     4561 ggataaaccc cgtcgccgtg aggcggctgc aataccacag ccggacgaaa acaattcgga
     4621 gatgccaccg tccgcggtga tgtctcctgg tattgaatat atggaggatg gtcttcccga
     4681 tccggtgaaa gcgatggggc gtcttctggt ggagaacatt aataccgacc ccaggctggc
     4741 gctggatgcg gcttataagc tggcgcagtt cacgcaccat aaaaaagggg atgccggtaa
     4801 aaaatcggca aaaggtgacg cggcgaaaaa agcggctaac cgttttgcgg tgccaccacc
     4861 accccgcctg gtggtgaata atgataatga gggcaacgga tgatacctgt gtggagcacg
     4921 gcctgcccgg actgggcaga gcgcctgaaa aaggggctgt cgat
//
LOCUS       DAZFZC010000205         4956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFZC010000205 DAZFZC010000000
VERSION     DAZFZC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.207"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..432)
                     /locus_tag="ACHKXP_005004"
                     /pseudo
     CDS             complement(1..432)
                     /locus_tag="ACHKXP_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
     gene            complement(435..1400)
                     /locus_tag="ACHKXP_005005"
                     /pseudo
     CDS             complement(435..1400)
                     /locus_tag="ACHKXP_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(1454..2211)
                     /locus_tag="ACHKXP_005006"
                     /pseudo
     CDS             complement(1454..2211)
                     /locus_tag="ACHKXP_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
     gene            complement(2225..3439)
                     /locus_tag="ACHKXP_005007"
     CDS             complement(2225..3439)
                     /locus_tag="ACHKXP_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFO7649806.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(3635..3973)
                     /locus_tag="ACHKXP_005008"
                     /pseudo
     CDS             complement(3635..3973)
                     /locus_tag="ACHKXP_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1444 a         1060 c         1054 g         1398 t
ORIGIN      
        1 gtcgccagtt catcaaccgt tttatgaatt ctctggcttt caaatttttc caggtaggga
       61 ccggaggaag caacaatatt cagacctgtt tttaatgcga cttctcgtaa tgcctgcgca
      121 tctcgtccga tagactcaga ccctgtggca tcaacaatgg tgcgaccacc gagcgagata
      181 aagttattaa tttcgaagat cacatcttca ataggtttgc ggtccatatt atcagcgcag
      241 caataaggat cgtgttttaa tgcccactgg atttctgccg tgactttttt tatcaacaag
      301 ccgttgtgaa aaaggatagc aaggagcatc cacaacagag gaaagatcgt tgaacagatg
      361 ttcatgagga agtgttagcc ccatatcttc acgggcgacg gggcccgtca cagtttgtag
      421 ataatctttc atgattatac ctccattttc ttcattgttt tcgaggttct gtattccgga
      481 atagtggtaa caattgcacc gaccagtgca aacagtgttc cgatgatcgt gaccagataa
      541 acgctattgc ccagcgatgg aattaatacg tcaatcaata ctgatcccag taactggccc
      601 gccgttgaag caacccccag catcagtaac cccagacctc tgaccaaaat ggccattaaa
      661 ccgatagaca ttagtccaag cgggccgcca agatacatcc accagacatc tggtaattga
      721 acagtgacgt gccctaatgc gacacgaata gctagcgctg tgcctaaaac acaaaatcca
      781 acgatgaaat tctaggtgat tgaaaccagc atggatcccg tagcttcagc tacttttgca
      841 tttccagcgg gctgccatcc tgcaagcaaa ccagctaaga acggaagaat ggcaagtaag
      901 atgaaagagg tagaatgcca ctgaggtgac acaacaaaaa tcgtagcaat aaccgcaaat
      961 aatgcaccga tgatgcgata aggggtaaag tattttttct cgtcgacacc gataccaaag
     1021 cgatcgcaaa gtaaaccaga cagcaatagc gcggagatca gtgcggtttg aaacgttgcg
     1081 accccgagtg cgctggctga tgcgccttca gaaaatacca ccatcgctcc acagagaccg
     1141 gcaaaccagt tccagagagg aacttttcgg tttttaatca gagaaggaat tgccgcaaat
     1201 tgttgccggg tttcttttcg cgccataata ataaaaaaca tcacgactaa accactggca
     1261 aaggagataa ctgcgcatgc attaccatcc tgtaaccagt gacctaattg accattcacc
     1321 gctgattgca taggggagag catacccgca agaatcgtgg caagcatcca taagggagtt
     1381 gagtacttat tctttttcat acatcaatcc ttctattata gtaaatggag taactggagt
     1441 taccccatta ttattatctt gcaatatgag tcagtatatt tacccacaac gcggtataga
     1501 acggccagga cataaactgc tgtgtacccc agtgcggtga acagtcactc ataaagcagg
     1561 ctattttccc gttgtgatag ttaccaaaga ccagtaatgg atcgttgttt attgttaata
     1621 ccacctcagc attttcttta gcgatcgccc ggttataacc taggaaaaaa ggatattcag
     1681 agaacccttt aataactgga tgttcaggtt gtgatggctg tgcaataaca ccctctggtt
     1741 tttcgacacg atcgtcacca tctaacatcg taaccgggag cacatcagcc agaactgtat
     1801 ttttataatt ggcttttgct tcaactccca tgaatgagag atagccgcca atcatcaata
     1861 acccaccacc gttattcaca tattctttaa tcagttcgag agcgtttggt tttatgcgaa
     1921 gctgataaaa agtatcattt tgtagcagga aagtattact accaatatca ctaataacga
     1981 tggcatcata ttgttctaac tgtgcacatc ttcagggaag gcaatttgta ccgtatgtgc
     2041 tggcatatag gtgacatcta cctgactatt tttcagacat tggagtaacc aggtcgcacc
     2101 ttcttcatat ttacttgagg taaaactgtc atatcctttt gaatgaatca tatgaatatg
     2161 ccaggattcc ccaataaaga gcacttttag tgattttttg gtgttattca ttctctgtcc
     2221 cttcttaatg cgtaaaaata gtttgctgat aatgtatact ctgaattcga tgtatcacag
     2281 attcatgttc aggcatgtcg gaagcattgc ttgtctccac cgctagggat gcaaaggcgc
     2341 tggcataaca cagtgctgat tccagtgatt ttcctttcgc aagtcctgat gccagagcgc
     2401 cgttaaaagc atccccggct cctgccgtat ttttaacgac cgctggaaac gctggcgaat
     2461 gaatgaattt tttgccatca aatgcgagcg agcccttgct accgagcgtg ataaccgtgt
     2521 tttttacccc tttattgtgg atagcttctg cagctcgttt agctgattca agatcgtgta
     2581 cgtctatgcc ggacagtaaa ccagcttctg tttcattggg ggtgatataa tcaacatcct
     2641 gaataagttc gttgacgata tcattatagg gtgccgggtt aataatgacc gggatactgt
     2701 ttttttgcgc caatgtgatc gcttgttgca gagcggtata atttgtttcg agctgcagaa
     2761 gtatgacatc agaattaata atcgcttctt tctgaatagt tatttcatcg gacgacattg
     2821 tcatgttggc accgggatag atcgcaataa cattatcgcc agtaccttca ttaacaagga
     2881 tcgtggcagt tccggtttgc gtttctttcg tttgataaat tatactttta taaatcttag
     2941 acgaattcat aaagttgatc gcataatcac taaagtgatc tgtacctact tttgttatga
     3001 aataaacatc agtatcggca aagcttgctg ccagcgcctg gttacagcct tttccccccg
     3061 gtgaaaatat aaaattactt gccagaagcg attctccgat agtgggaagc ctcggcaaat
     3121 aactgatgat atcaacatta aacgaaccaa taacgcatac ttgacttttc atagttgagt
     3181 ggcctttttt tattccctgg gctgtatcat aattttcaag cgcataagcg atttcatttt
     3241 tcgcaatagt atcaagcgag tctaggacga taaaagcacc gccatgacat cgagtcagat
     3301 agccaagcct ggcaagttca ttaagatctg aacgaatagt ttctttcgtt acattgaggc
     3361 tattggacag atcggatact tttacagaac cgaatttact gagtcgtttc aatatttcat
     3421 gatggcgttc aaatttcatg cttcacctca cttcagttac gttaatatct atcgatgtat
     3481 attgatgtga taatcaccat aaagtggcgg ggaaaagcaa aaaaaagcaa aaatagcctg
     3541 agaaaagata agcgttaatg gagaatcata ttgatgctta tttattggtg agaactacgt
     3601 tccatggcag gagttcatca atacggttgg aaggttattc cggcagtacg ctcaggatat
     3661 ggcgcagata cgcttccgga tcaatgtcgt tcagaaggca agtgccgata agcccataca
     3721 acagtgcact gcgctcgccg ccgaagaaca taaagttttt atttccgaga cagatggcac
     3781 aaagggcttt ttccgcggca ttatgtctgc ctccgcctga ccgtcaacac tgtaataaca
     3841 gagtgcgccc cactgattca gtacacagct gaaccattct cccagtctgg atttttggat
     3901 agcgtggcat ttttctccac catgcatgca gtgagtccag taacagtttg ctcttcttct
     3961 gcctgaccac aagatgtgct ggactggtaa acgatgggga tacctgctta cgcgagagca
     4021 ataagtggct ggccacaatt ctcggtacaa aactctgctg aatagcagcg attttcccag
     4081 cgtggtgcgt cgtttgcatt ttactggttc taagttaaca attatctgtt cactaatgac
     4141 tactctaaaa acttgtatca ggactatcac ttatttaagt gatattggtt gtctggagat
     4201 tcagggggcc agtctataaa ctttttacaa aaggatgaat gcagaaggtt actctaatgg
     4261 ccagagtgat gacaatgatg gtcagacgtt gacctgcaga aactgcattt taattggtct
     4321 ataaagtctg caagaggaat cagggtcaga tcagtgagtc ttatcgcggc ccgaagctat
     4381 gactgaaaga aggctggata cagaattgca acaatttggc aatgtcagaa cacagaagct
     4441 ttgcaagcag gtgaaaggtt atgtctagca aaaggagtat atcagaatat atctactgat
     4501 gtacaaaaca caacaatagc ttcatgatat ataaatacgt gatgacgagt ggtactccgc
     4561 atggttattt aacttctcag caccactaat gatcctgagc aacttcgtat ttgggccatt
     4621 gccatggagt agaaagctgt gtctgagaat gcagaatttg agaaaagaat tcgctttctt
     4681 ggagagcttc tgaaaatggc acgctaacaa cgttttgtta aaaaatgtga atcacttgcc
     4741 ggggctgcat tttctattcg aggagaatgt tgaggtagat attacagaaa tctcagcgca
     4801 tcaggataaa ctgctacctt aggtcaacca ccaaaagaaa atacaatacg tccagtttgt
     4861 atgcctttaa taacacatct tctaaatgtt gaagaattat cccgcctgct gaagaacgtt
     4921 gctctgagtg tgataaacct ttttattctt gatgat
//
LOCUS       DAZFZC010000206         4837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFZC010000206 DAZFZC010000000
VERSION     DAZFZC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.208"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..379)
                     /locus_tag="ACHKXP_005009"
     CDS             complement(<1..379)
                     /locus_tag="ACHKXP_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO7649807.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVFFVENES
                     WGSVIYNLFLQLEKENKSHTSLEV"
     gene            complement(370..934)
                     /locus_tag="ACHKXP_005010"
                     /pseudo
     CDS             complement(370..934)
                     /locus_tag="ACHKXP_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1099..2058)
                     /locus_tag="ACHKXP_005011"
     CDS             complement(1099..2058)
                     /locus_tag="ACHKXP_005011"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO7649808.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGW
                     FRHQDMAEGYLVMKSLDQEICSRDRSKMGDNVWCCITAQASHS"
     gene            2068..2301
                     /locus_tag="ACHKXP_005012"
     CDS             2068..2301
                     /locus_tag="ACHKXP_005012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649809.1"
                     /translation="MSQQPVRLRVSGGIPQVIKCRTVTEVNGGGIFQREEQRMCLTCP
                     CSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(2233..2436)
                     /locus_tag="ACHKXP_005013"
     CDS             complement(2233..2436)
                     /locus_tag="ACHKXP_005013"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO7649810.1"
                     /translation="MVLSDCYSLANDQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            2614..2787
                     /locus_tag="ACHKXP_005014"
     CDS             2614..2787
                     /locus_tag="ACHKXP_005014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649811.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            3359..3908
                     /locus_tag="ACHKXP_005015"
                     /pseudo
     CDS             3359..3908
                     /locus_tag="ACHKXP_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            4106..>4837
                     /locus_tag="ACHKXP_005016"
     CDS             4106..>4837
                     /locus_tag="ACHKXP_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HFO7649812.1"
                     /translation="MASRLESFLNDDCFVWYDIPVGRKNRHPDFVIIDPDNGLVFLEV
                     KDWTVSTLRKANQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPADPCLRQNDGQY
                     KGRLNLAWAYGVVFTRITRQQLKALTGNNENAVEKISPSAQTICQDEMTQSVLPEVFR
                     QKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILARNIGDGHR
                     VIHGVAGSGKTMILLFRCLYLAETTS"
BASE COUNT         1277 a         1000 c         1169 g         1391 t
ORIGIN      
        1 gaacttctaa tgatgtgtga gatttattct ctttctcaag ttgaagaaaa aggttatata
       61 taacgcttcc ccaagattca ttttcaacaa aaaacacatc acccttaata ttatttgtcc
      121 ccttgttttc ctgaattaca atagctttgc ttaataattg aggggatgaa aatgtttcgt
      181 agtctatctt tcctgaatta ctacaaaaaa actcactcca atatgatgct atatgtcgac
      241 accatatgat ttccccattg caatcactaa cctttccatt tagatttata ctggcatctc
      301 tcttttttga taaataaggg ggattgtttt ttgaaatata ttcaacataa tttgatgccg
      361 atttgtttat tattggcata tgtttgtatc ctttctataa attttccgcc tgacagtgga
      421 aataatatat tctactgatt tttgtagttt aaacgattta tgaagaattt tactgttgta
      481 cagtaccaga tactcagcca gtttcagatt gaacatattc agctattcaa agagcaattc
      541 ttcattaaac tcaataaatt gttccctcag tgttcggtta aaatgctcac ttatggcatt
      601 taattttcgg cgtatagaga taagtccata cgaacccgac ggtggcttcc tgtagcgttt
      661 tgtcgaattt tcccgagaac tctttgccca tcatcagtga tgatctggtt gataccaaca
      721 aggaacaact gggctacaca gcagaagaaa tgcctgatga tatcactttt aagcgatagt
      781 acggccagag agtagttgct gtgttcgtcg agcatggtaa tgaaataacg gtacagctcg
      841 ccattctgag ctcaatagta tccatgccac tgagcttaca ggtctttatc gggcgatagt
      901 gctttattct tcggggcttt gtggatgatt tttcgaaatg ttcatgatag ttaggtaaag
      961 ctaccgcata tcatttgcaa aatgacagat agatatactt caatttgaat cttaacaatt
     1021 agtatgggaa tgctgtcaat cctctgtatg cgtccaccac atattgaaat ttttgttctc
     1081 taatatattc cacgggcgtc aagagtgtga ggcctgtgct gttatacagc accagacgtt
     1141 gtcccccatc tttgacctgt ctcttgagca gatctcctga tcaagagact tcatcaccag
     1201 gtaaccctca gccatatcct gatgtctgaa ccagccatcc cacataacta cccaaccggg
     1261 gctgcttgtg cgtttgctgt catgccatcg ccccagttta gccagtttca aacaggccca
     1321 tttcagggtc ggtgtttgcg ccggaagctg ctttccttcc agcttaaccc acagtagttt
     1381 ccactctgtc ggcgtcagta ttttctcaca gctgtcattt tgtgtttctt cactgatacc
     1441 tccctgccgc aggcccagca cccgcaccgc gataaccacc atgcgctcaa ggttatcccg
     1501 ggtctgcatt cgcagcgatt ccacacatgt accaccactt ttccacgctt tgtggtattc
     1561 ctctatcagc cagcgttgct cgtaatggct gacgatacgt cgcgcatcgg cggcactcgc
     1621 cactttttct gatgtcagcg gataccagca ggctccgtcc tctcctgctc cccgcaacag
     1681 acatacgtga gcgagagcgc ctggccgctg ttgtcgggat tttttatgct gacttcgctg
     1741 taactgatga gcatccgggc ctggcgggct gcccgcccgc ctttttgcat cacattcagc
     1801 gtgtggcttc ccgcggctga ctttataatg caggtaatgc cagatatccg cttcccggtc
     1861 acagacagtg attacctgtt tctgtatctc ccccagccgt tcggccatgc gctccgaagc
     1921 ctgctgccag cggtaacttt ctttttcttc acagggacgt tcttttcgct ggtgcttaac
     1981 accgtaggtg tccgtggcac gactccagca ctgctgttcg ataagatcga ctggcagggc
     2041 gctgtcgggg gcgtacatca ggacagagtg agccagcagc ccgtgcgtct tcgggttagt
     2101 ggtggtattc cccaggtcat caaatgccgt actgtgaccg aagttaatgg tggtggtatc
     2161 ttccagcgcg aggagcagcg gatgtgcctc acatgccctt gcagtggcag taaatccggc
     2221 ttcggcaatg gcttacgggg acaccgacgg gttacgtatc aggcggtacg caccttcaac
     2281 ctgagcagtg aagtgggatg atttcacaat agaaagacct gcatgctgag cgagagaaga
     2341 ggtcagtgac acaaggcgtc gtgtacgacg cggatcaccg agacgggcat gtccagactg
     2401 atcgttagcc agtgaataac aatcagaaag taccataaca gacccgaaga aaatgaaata
     2461 taagagaaga tcaacgggtg aagaaaaagt tcaaaaaatc gctgccgggg aggaaggaaa
     2521 ctaccggatg gaaagagtcc ccctaaagca gactgacaga catcacaaat ccccgggtgg
     2581 ggatttgtgt ataagagaca ggttacaggt gtggtgaata agactgcaaa tgtggcagcc
     2641 catgccgtgg tgaatgctgc actggcggtg gtacagggaa ataacgcccg tgccagtgcc
     2701 gcaggtgctg ccactggaga cgtggtgggg atgatagcca cagagatgtg cggaatatcc
     2761 cctggcactg ttacatgtaa aagataaata acgaagctac atccttctct ttatgaacgg
     2821 ctttatatta gttttgctga caagattgag ctaaatccgg ccaatgaaga agagcaggtt
     2881 attctttaaa ggtttgataa catgtaacac attttgaata ttcagtcagt aagccttaaa
     2941 aatttacctg gttatgggct gactgatata acaaagtaaa tattcgatgg ggaatgccgg
     3001 atgcaatgaa caatgcatta acgtatcgaa ataatgatgc aggagcaaac tcagtctggt
     3061 gagtatacat tatgccattg atgcagagtt taaaggaaca gggctggttc gttatggaac
     3121 aacatttgtg cctgaggaaa gggggggagt atctgaggca acagaagttt gtggggagtt
     3181 cggtttatta ccatgcttgg ttttttgtca gtgtgttcaa atcgtcaggt gatatattta
     3241 tagttttttg atgatgattt caaataatgt ttttgagatg tgtggtcctt tttgattttc
     3301 agaaatcatt tattaatgaa ggagggttaa atgaaaaaaa ctcgttataa tttcagtaat
     3361 gatattatct gttatgagtg gttcaagact ggctgataat catacgctat cggctggtta
     3421 tgctcagagt aaggtacagg actttaagaa tattaaagga gtgaatctgc aatatcgata
     3481 tgagtgggat tctccggtca gtgtcgtcgg gagtttttcg tatatgaaag gtgattgggc
     3541 ggacagtcat cgcgatgaag ctgacgattt ttacagacat caggctgata ttaaatatta
     3601 ttcatttctc gcaggcccag cttatcgtct gaatgactat atcagttttt atggtttggt
     3661 ggggatatct catactaaag cgaagggaga ttatgagtgg agaaacagtg tgggtgctga
     3721 tgagtctgat ggatatttaa gtgagtctgt atcaaaaaaa atccactgat tttgcttatg
     3781 cagctggtgt tattattaat ccgtggggca atatgtctgt aaatgttggg tatgagggaa
     3841 ctaaagcaga tatatatgga aaacattcgg tcaatggatt tactgttgga gttggttatc
     3901 gtttttaatc attagacggg actggataat tgcacttgcg tagtgaagtt tactgaatgt
     3961 ttgtatttta atgaagtatt cgacatattc ccttcagacg gaatatgtcg acggttatgg
     4021 ttttactgga gcatgagtat gttatggcga aactgataaa tatatcaggt ccactgagga
     4081 gagaatgacg ccaggggaga ggagggtggc ttcacgactg gagagctttc tgaatgatga
     4141 ttgctttgtt tggtacgata tcccggtggg acggaaaaac cgacatccgg attttgtcat
     4201 tattgacccg gataacgggc tggtatttct ggaggtcaag gactggactg tatccacatt
     4261 acgtaaagca aatcaggagc aggttacact ggaaaccgac ggtcttctga aatcagaaat
     4321 aaatccactg gtacaggttc gtcgttatgc ctgtgatacg gttaatgcct tacctgctga
     4381 cccgtgctta cggcagaatg acggtcagta taaggggcgg ctgaacctgg cctgggcgta
     4441 tggtgtggtg tttacccgca ttacccgtca gcaactgaag gcactgacag ggaataatga
     4501 gaatgcggtg gaaaagattt ctccttcagc acagacaatc tgtcaggatg aaatgacgca
     4561 gtctgtttta cctgaggtat tccggcaaaa aattgccggg atgtttacca cggggttcag
     4621 aacccgggta acaccccgga tgagggatat tctgcgcgcc catctgtttc ctgaagtgac
     4681 cgtgaaacag aactcacaaa tcaaaattat ggatattcag caggagattc tggccagaaa
     4741 tattggtgac gggcatcgtg tcattcatgg tgtggccggt tccggaaaaa ccatgattct
     4801 tttgttccgt tgcctttacc tggcagaaac cacgtcc
//
LOCUS       DAZFZC010000207         4821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFZC010000207 DAZFZC010000000
VERSION     DAZFZC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.209"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..620
                     /locus_tag="ACHKXP_005017"
     CDS             <1..620
                     /locus_tag="ACHKXP_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HFO7649813.1"
                     /translation="LMLVKTIAELNMARESGLYILVEFIGNPITAMFIAVFVAYYVLG
                     IRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVILSNMHMHP
                     ILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGSGAIGCTIV
                     TDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            638..1970
                     /gene="dsdA"
                     /locus_tag="ACHKXP_005018"
                     /pseudo
     CDS             638..1970
                     /gene="dsdA"
                     /locus_tag="ACHKXP_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(2078..3616)
                     /gene="emrY"
                     /locus_tag="ACHKXP_005019"
     CDS             complement(2078..3616)
                     /gene="emrY"
                     /locus_tag="ACHKXP_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO7649814.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIVVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIIMPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(3616..4779)
                     /gene="emrK"
                     /locus_tag="ACHKXP_005020"
     CDS             complement(3616..4779)
                     /gene="emrK"
                     /locus_tag="ACHKXP_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO7649815.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1304 a         1103 c         1085 g         1329 t
ORIGIN      
        1 cgctgatgtt ggttaaaacg attgccgaat tgaatatggc gcgtgagagt ggtttgtata
       61 tcttggttga gtttattggc aaccctatca ctgccatgtt tatcgccgtg tttgtcgcct
      121 attatgtgtt gggtatacgc cagcatatga gcatggggac gatgctcaca catacggaaa
      181 atggcttcgg ttctattgct aatattttgc tgattatcgg ggccggaggc gcattcaacg
      241 ccattttaaa aagcagcagt ctcgctgata cgctggcagt tattctctcc aatatgcata
      301 tgcacccgat tcttctggcc tggttagtgg ctcttattct gcatgcggca gtgggctccg
      361 ctaccgtggc aatgatgggg gcaacggcaa ttgttgcacc catgctgccg ctgtatcccg
      421 acatcagccc ggaaattatt gcgattgcta tcggttcagg tgcaattggc tgcacgatcg
      481 ttacggactc gcttttctgg ctagtgaagc aatattgcgg cgctacgctc aatgaaacat
      541 ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg ggcacattcc
      601 tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta aaatgaactc
      661 gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa ccacctggtt
      721 taatcctggc acgacctcat tggctgaagg tttaccttat gttggcctga ccgaacagga
      781 tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa aagcatttcc
      841 tgaaactgct gccactgggg ggattattga atcagaactg gttgccattc cggctatgca
      901 aaaacggctg gaaaaggaat atcaacaacc gatcagcggg caactgttac tgaaaaaaga
      961 tagccatttg cccatttccg gctccataaa agcacgcggc gggatttatg aagtcctggc
     1021 acacgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg actacagcaa
     1081 actgctttct ccggagttta aacagttctt tagccaatac agcattgctg tgggctcaac
     1141 cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta aggtgacagt
     1201 tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc atggcgttac
     1261 ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta aagcagcgca
     1321 gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt tccttgggta
     1381 ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta tcgtcgatgc
     1441 tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg gtggcgtcgc
     1501 attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg aaccaacgca
     1561 ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt ctgttcagga
     1621 tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg catcaggctt
     1681 tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg atcaaaccat
     1741 gtatgacatg cttggctggc tggctggctc aggaagaagg tattcgtctt gaaccttcag
     1801 cactggcggg tatggccgga cctcagcgcg tgtgtgcatc agtaagttac caacagatgc
     1861 acggtttcag cgcagaacaa ttgcgtaatg ccactcatct ggtgtgggcg acgggaggtg
     1921 gaatggtgcc ggaagaagag atgaatcaat atctggcaaa aggccgttaa taacgtttca
     1981 acgcagcatc gcaatccttt ccctgggtga gcgatgctgc cgatggcgca gacttaagat
     2041 ccccggtctt acccgctata accccctcct tttatcatca cccaacgcct ttcgctgtaa
     2101 acggcggttt cgcaaaccaa accaaaaccg taagtaagat aaaagcaata gccgccatac
     2161 ggaaaatctc atttgcagaa atagaaagtg actgctgggt tatttcatta ttaatttcat
     2221 taagaactcc tgaaagcgaa ccatagtatt tatccataat ttgtgacgat gaattaaaca
     2281 cggggttaaa ttgatcgatg gttgctgtca actgactatg gtgtaacgat tcgcgtcgtc
     2341 cccacagcgt cattgtcaac gacgtaccaa ctgatcctga caaggtacga aaaaaattac
     2401 tcatactcga ggcattggca aatttattat ctggcaagcc tgaaaacgaa atcgttgtta
     2461 agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa ctgcggcata atgatacctg
     2521 taaaatcaat cgttggcata aatgtcacag aacgccagta atagcaaacc gcatacatca
     2581 aaaaactgaa tgtcaccaac acccgcatgt ctattttgtt gccataacgt cctatcaaag
     2641 gtgaaattaa tagtggcatg atgccgatgg gcgcataagc aagtccggcc catatcgcat
     2701 tataccccat cgtttcctgg agtaactgcg gcataaggac gatcgctcca gagtaaaata
     2761 aatacgcgca tgtgatactc acaataccaa tggtgaagtt acgggactta aacaaactga
     2821 gatcaagaat cgggttctct gaggtcgact cccaaatgac taaagagatc agagaaataa
     2881 ctgatactac tgttaatatt attatcgtac tcgagttgaa ccaatccaga tcgcgccctt
     2941 tatcaagcat aatttgcaag ccaccaacac cgagcactaa cagggtcagt cctggtagat
     3001 tcattttgac cggtgaagtc tcagtttctc ttcctttaag taaggttaag cataatgtca
     3061 ggacgacaat ccccataggg acattgatta aaaatatcca accccagcta aagttatcac
     3121 aaatatagcc gcccaatatc ggcccacata tcggagcgat aatcacggtc attgaccata
     3181 atgccagagc aaatgtcctt ttttctggtg ggtaattcct taataataaa ctctgtgaca
     3241 gtggaattaa cggccccgcc attaacccct gaacgactct aaaaaatatc agcacatcaa
     3301 gattggtcga taggctacac attaatgaag acagcgaaaa aaaggttact gaaagtaaaa
     3361 ataatcttaa ttcgcctatt ctttgtgcca acctgccagt aacagggatc gcaatggcat
     3421 ttgctacacc aaacgaggtg ataacccagg tgccttcgtc tgttgatgct cccagaaagc
     3481 cagatattgt cggtattgcg acgttagaaa tagtggaatc caacatttgc ataaatgtcg
     3541 ctaatgacaa tgcaatagtg acgcaccata acgtcccacc ggttaatggt gccggagttg
     3601 atttagtgat tgccattaaa gttgtccatt atgcgaaata atgttgctaa tttctttttc
     3661 tatcggactg gtatcgataa ctaaagcctt actggtataa gccggcatgg aggtcacggt
     3721 tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc gtatcaatag ttgctgtcat
     3781 cgataaacca atacgcaagg gatgttccat gagttctttt ggatcaagag aaacttcaac
     3841 cggtacacgc tgaacgattt tgatccagtt ccctgtcgca ttttgtgcag gtaataagga
     3901 gaacgcattg ccggttccca tattgatccc tgtcacccga ccatgaaaca caacattttc
     3961 accataaaga tcgctgataa tattgaccga ttgaccaatc cgtacatccg tgagttgtgt
     4021 ttctttaaag ttggcattaa cccacatttg acgtgccggt acgacagcca ttaacgattg
     4081 tccggggctc actgtttcgc cgacctgaac acttctctgg gcaatatagc cggtaaccgg
     4141 actcttaata tccgtacgtt taagcgccaa ccaggcttct ttagttgcat ccgccgcttc
     4201 aacgacttgt ggctgacggt ttaatggtgt gttcattact aaagctttat tcgctttata
     4261 agcctggata gcggcattca atgccgcttt gctacttatt aacgtatctt tggtatgctc
     4321 cagcgtttct tttgaaataa ccccctgctt cgctaacggc actcgacggt tataatcttc
     4381 taaagattgt tgatactgaa tacgtgctga agcgacttcg gcactgtatt gtttatcctg
     4441 taagtatagt ttattcgttt gccgaacaat atttgccaga ttatttttag ctttattgag
     4501 tgcgatagtg gcatcagttt tatccagtga aactaaaatg tcaccttgtc gaacgtagtt
     4561 cgtatcttta tgattaacga cagtgacact acctgagact tgtgcagaaa ttggatctgc
     4621 attccccgtg acataggcgt catctgtact aatcatgtct tctaattcca ttgaccaata
     4681 ggcataggca cctgaaaacg caataaataa aactaccgcc aataaagaaa agtattttct
     4741 tctgttagaa tgttttttat ttgaattaat ctgttccact attatctctc atttctcata
     4801 gatgaaattt atgaattgaa t
//
LOCUS       DAZFZC010000208         4794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFZC010000208 DAZFZC010000000
VERSION     DAZFZC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.210"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..3500)
                     /locus_tag="ACHKXP_005021"
     CDS             complement(<1..3500)
                     /locus_tag="ACHKXP_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017893420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing NTPase"
                     /protein_id="HFO7649816.1"
                     /translation="MLNRKVRQIYPQQGKDTQTVVFSDYSHFPNIVLLGDPGAGKTHL
                     FKQFAALQEAKYLTVRHFLSGVPIDNQKTLFIDALDEKRSASNSSDVVDEIVRRLFSQ
                     TPQNVRISCRSQDWLGESDLSAFLPYFEQTGGYVVLHLQSLSKEEQTVILQANGVEHP
                     EAFLAEAEKHAASEFLYNPQNLLMLAEAVKEGNWPRTRSELFHSATQFLLTEHSQEHT
                     RLTSGIYSCDELEYPAGSICALCILSDVSGVSLLPNDIRPEYPSYRTISFFSHEIIRA
                     ALSRRVFSAGDELETVGYSHRTIAEFLAAKWLAGIVDKGLPMGRLRSMLGFEGYPSSE
                     LRGLHAWLAVFLPQYAQVFIEADPYGVLTYGDAASLPTVEKQNLLVALSKLSERDPWF
                     RNHGLNSYLNGFVTVEMETLLRQIVRDPHSSFSLRMIVLESLSVTTPIVALSDDLINI
                     AKSKDFSYAEKEEAITALIHFGVTGQKFLVSFFAELKDKSHDSIRLRNHIIQLLYQDY
                     FVPQDIAQLLVDTLNITNERLPIGSLWRLNDIVPTADLLAIFEHLDRYHTAHSNDWYS
                     SAKNYSEIVHFLEAGLLQILSEKSGYSASQIWLCLKVFYSYSHNHHYRSYDNVQKSIV
                     QEIKNRSWQYEDIIDAAIISFTRYDTRYLFLHEFSESTFHLVPDKLLLARFIHYLSHT
                     QSDSEKIECIYRLAFATLYRCDTPPQDTFDFLSAYCHSNEQLLTVFDTTIICEIDDWR
                     IKQNLSRIENKKKQEEVRCEDRLNFELCRQEIVSGQHLEWLKHISYIYYSKYSDVDDK
                     LTPMQRCADILGAENVTDAISGLLAILNRSDLPTIDEVNCALLKREYYKWWYAVLAGS
                     DELFKKHPDVTQWNEDLLTTLLALDVILALSTKDNNVVSDYSLPWKRDVILNKTAFFI
                     KTYYHIINFCINNQLQEIRALNYLLNDTYIPIDYSVPLVMRLAKEHPDDVLHLQNLMG
                     WLLGHPESHAELLQLSELMIAQPSKLKKPTYQIWLVCAYLFAPDKCQDLFLSESKSTA
                     EMIALIRQLSGKAKRGHGSNEQRLSLTQLEAIITVSATHFPNADRVASNDSNLANNWE
                     YADFIKRLIDDVSSFSSYDAGEVFERLLLLPECTSYRDYLLHAQSNQKARYRESQFQH
                     ADWKQALNTLTNQTPTNVMDL"
     gene            complement(3843..4787)
                     /locus_tag="ACHKXP_005022"
     CDS             complement(3843..4787)
                     /locus_tag="ACHKXP_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO7649817.1"
                     /translation="MDLIMEWRFLGSLSEARKSGCSGVYLIVHKGLFSRVVYVGVSCN
                     VGRRITEHYDGYLRGNRTIYDAGHDEDVYRFMSAYKIHNHTKYYQALAKDYKIWASTT
                     MYSDLPKNMLAKSQTFDTDWQSIALGKYIPQLVVWALPMAKYCYSNASRIESVIQSKL
                     IKSFDLRGFFNIKQLSILGKIEYPYMGKVKVFIINTPDLDPASQLIFSNLYNKKTDEN
                     FCKEFRSQFKSEIFQRESETQRKRTIREHKVSLYENYGKPWTLKEMEKLRVMLVDFDL
                     SPTEISEYLGREPRSISKKISENDKVTNYKWRESVGWL"
BASE COUNT         1388 a         1020 c          925 g         1461 t
ORIGIN      
        1 agatccatca cattggtcgg ggtctgattt gtcaacgtat tcagtgcttg tttccagtcg
       61 gcgtgttgaa actgtgattc ccgatatcga gccttctgat tgctttgggc atgcaggaga
      121 taatcccgat aacttgtaca ttctggcagt aataataaac gttcaaatac ttcaccggca
      181 tcgtatgagg aaaaagaaga aacgtcatca atcaatcttt tgataaaatc agcatactcc
      241 caattgtttg ctaagttaga gtcgttactt gcaactcgat ctgcattagg aaaatgagta
      301 gcagagacag tgatgatcgc ttccaattgt gttagtgata aacgctgctc attactccca
      361 tggcctcttt tcgccttacc agatagttga cgaataagcg ctatcatttc tgccgtggat
      421 tttgattcag ataaaaatag atcctgacac ttgtctggag cgaataaata agcacatacc
      481 agccaaattt gataagttgg ctttttaagt tttgagggtt gcgctatcat caattcagat
      541 aactgcaaca actctgcatg actctcagga tgccctaaca accaccccat caggttttgt
      601 aaatgaagaa catcatcagg gtgctcttta gctagtctca taactaaagg aacggaatag
      661 tcgattggaa tgtaggtatc atttaacaga tagttaagtg cacggatctc ttgcaactga
      721 ttattaatac agaaattgat aatgtggtaa taggttttga tgaaaaatgc tgttttgtta
      781 agtatcacat cccttttcca cggtaatgaa taatcactta caacattatt gtctttggtg
      841 gaaagtgcta atatcacatc cagcgccaat agtgttgtta acaaatcttc gttccattgt
      901 gtaacatcag gatgtttttt aaataactca tcgcttcctg ccagtaccgc ataccaccat
      961 ttgtaatact ccctcttcaa caaagcacaa ttcacctcgt caattgtggg cagatctgaa
     1021 cgatttaaaa tcgctaaaag accggatatc gcatcagtga cgttttcggc acctaatatg
     1081 tctgcacagc gctgcattgg cgtcaatttg tcatcaacat cgctgtattt tgaatagtaa
     1141 atataggaaa tatgtttgag ccactctaaa tgttgacccg acacaatttc ttgacgacat
     1201 aactcaaaat tgagtctatc ttcacaacga acctcttcct gttttttctt attttctatt
     1261 cggctaaggt tctgctttat tcgccaatcg tcaatttcgc agatgatggt agtgtcaaac
     1321 acagtaagta actgctcatt tgaatggcaa tatgcagaaa gaaaatcaaa cgtatcttgg
     1381 gggggcgtgt cacaacgata aagagttgca aatgccagtc gataaataca ttcgattttt
     1441 tctgaatcac tttgggtatg ggataaatag tggataaaac gagccagcaa aagcttatca
     1501 gggacaagat ggaaggtgct ttcagaaaat tcatgtaaga acaagtatcg agtatcatag
     1561 cgtgtaaatg agataatggc ggcgtctata atatcttcat attgccagct acgatttttt
     1621 atctcctgaa cgatagattt ctgcacattg tcataactgc gatagtggtg attatgggag
     1681 tagctataaa acacttttag gcacaaccat atctgagatg cgctgtaacc actcttctcg
     1741 cttagtatct gcaataatcc agcttccaga aaatgcacaa tctcagagta atttttagct
     1801 gaagaatacc agtcattact gtgagcggtg tggtaccgat cgagatgctc aaagatagcg
     1861 agcagatcag ctgtcggtac aatatcattc agtcgccata atgatcctat cggtaatcgt
     1921 tcgttggtta tatttaatgt atcaaccagc aattgtgcta tatcctgcgg tacaaagtaa
     1981 tcttgataga gcaactgaat aatatggtta cgcaaccgga tgctatcgtg acttttgtct
     2041 tttaattccg caaaaaaact taccaaaaat ttctgaccag tcactccaaa atggattaat
     2101 gccgttattg cttcctcttt ttccgcatac gagaaatcct tcgattttgc aatattaatt
     2161 aagtcatcac tcagtgcgac tatcggtgtt gttaccgata aggattccaa aacgatcatg
     2221 cgcagagaaa atgacgaatg tggatcgcgg actatctgtc gcaacagagt ttccatttct
     2281 actgtcacga agccattcag atatgaattg agaccgtgat ttctaaacca cggatcacgt
     2341 tctgaaagtt ttgacaacgc caccagtaaa ttttgttttt ccactgtcgg taatgaagct
     2401 gcatcgccat aggtcagaac gccgtaagga tcagcttcaa tgaagacttg ggcgtattgc
     2461 ggtaaaaaga ccgctagcca tgcgtgtaaa cctctcaatt ctgaagacgg atatccctca
     2521 aatcccagca tacttctcag acgtcccatg ggtagccctt tgtcaacgat cccggctaac
     2581 cattttgccg ctaaaaactc agcaattgtc ctgtggctgt acccaacagt ctcaagctca
     2641 tctccggcag aaaaaacccg cctcgataac gccgctctaa ttatttcatg tgagaagaac
     2701 gagatggttc tgtaactggg gtattctggt cggatatcgt tcggaagcag agaaaccccg
     2761 gaaacatcac tcaatatgca caacgcgcaa attgatccag caggatactc taattcgtcg
     2821 cagctataaa ttcctgaagt tagccgcgta tgctcttggc tatgttcagt taaaaggaac
     2881 tgtgttgcag agtggaaaag ttcagacctt gtacggggcc aatttccttc tttaactgct
     2941 tcagccagca ttagcagatt ttgtgggttg taaagaaatt cgctggcggc atgtttttct
     3001 gcttcagcca gaaacgcttc agggtgttct acaccattag cttgtagaat cacagtctgc
     3061 tcttcttttg agagcgactg caaatgtaaa acaacatagc ctcccgtttg ctcaaaatag
     3121 ggtaaaaatg ctgacaagtc tgattcgcct aaccaatcct gagagcgaca agaaattctg
     3181 acattttgtg gcgtttggga aaaaagtcta cggacgatct catctacaac atcactactg
     3241 ttactggcac tcctcttctc atctaatgca tcgatgaata acgttttctg attatctatc
     3301 ggcacgccac ttaagaaatg acgtactgtc agatattttg cctcttgtaa tgccgcaaac
     3361 tgtttgaaga ggtgcgtttt tccagcacca ggatcaccaa gcagaacaat gttcggaaag
     3421 tgagaataat cactaaatac caccgtttgg gtatctttac cctgttgagg atagatttgc
     3481 ctgactttcc tgtttagcat tctataggct cctgagtatc tatttttgtt ctaccaaatc
     3541 tgcatggcag tttttactcc aaatttacca tagactttca gccgatacag taaaccatct
     3601 aacccaccat ggttgctatg atgtcacgtt ttggtttaca tgacagactc atagtgagca
     3661 gaactggctg cttggacgtc gagtggtgat accattcaaa cattgatgga atccggaatg
     3721 acgctggctc cgttgcatgg gctgcatgcc gctcacagcg gaccttcaac tcagttagct
     3781 tgtccgttct gtgccaagag cggactttgc aaaatggggg ttatttcaat caaaacgtaa
     3841 cgtcacaacc agccgacgct ctctcgccat ttataattag taactttatc attttcgctt
     3901 atttttttag atatagagcg cggctctctt cctagatact cagatatttc tgtcggggac
     3961 aaatcaaaat cgacaagcat gactctaagt ttttccattt cctttaaagt ccaaggcttg
     4021 ccataatttt cataaagaga taccttatgc tccctgatag ttcgctttct ctgagtttca
     4081 ctttccctct gaaaaatctc gcttttaaat tgtgaacgga actctttaca gaagttttca
     4141 tcagtttttt tgttatatag attgctaaaa ataagctggg atgccgggtc taaatcaggt
     4201 gtgttaataa tgaaaacttt gacctttccc atatagggat attcaatttt acccaatatg
     4261 cttaattgct ttatattaaa aaaacctctc aaatcaaaag atttaatgag ctttgattgg
     4321 attacacttt caatcctgct tgcatttgaa taacagtact ttgccattgg aagggcccat
     4381 actaccagtt gaggaatata ttttcctaat gcaatgctct gccaatcagt atcaaaggtt
     4441 tggctttttg ctagcatgtt tttcggcaaa tcagaataca ttgtggtaga tgcccatatc
     4501 ttataatcct tcgccaaggc ttgatagtat tttgtgtggt tatgaatttt ataagctgac
     4561 atgaagcgat aaacgtcttc gtcgtgacca gcgtcgtaaa ttgttcgatt tcctctaaga
     4621 taaccgtcgt aatgttcggt tatccttcta cctacattac aacttacccc aacgtaaacc
     4681 acacgactga aaagtccttt atggacaata agataaactc cgctacagcc agatttcctg
     4741 gcctctgata gagaacctaa aaatctccat tccataatta aatccattat tatt
//
LOCUS       DAZFZC010000209         4599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFZC010000209 DAZFZC010000000
VERSION     DAZFZC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.212"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..418)
                     /locus_tag="ACHKXP_005023"
     CDS             complement(<1..418)
                     /locus_tag="ACHKXP_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7649818.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCD"
     gene            complement(533..608)
                     /locus_tag="ACHKXP_005024"
     tRNA            complement(533..608)
                     /locus_tag="ACHKXP_005024"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(573..575),aa:Thr,seq:ggt)
     gene            complement(615..689)
                     /locus_tag="ACHKXP_005025"
     tRNA            complement(615..689)
                     /locus_tag="ACHKXP_005025"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(654..656),aa:Gly,seq:tcc)
     gene            complement(806..890)
                     /locus_tag="ACHKXP_005026"
     tRNA            complement(806..890)
                     /locus_tag="ACHKXP_005026"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(854..856),aa:Tyr,seq:gta)
     gene            complement(899..974)
                     /locus_tag="ACHKXP_005027"
     tRNA            complement(899..974)
                     /locus_tag="ACHKXP_005027"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(939..941),aa:Thr,seq:tgt)
     gene            1336..2286
                     /gene="coaA"
                     /locus_tag="ACHKXP_005028"
     CDS             1336..2286
                     /gene="coaA"
                     /locus_tag="ACHKXP_005028"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO7649819.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2315..3280)
                     /gene="birA"
                     /locus_tag="ACHKXP_005029"
     CDS             complement(2315..3280)
                     /gene="birA"
                     /locus_tag="ACHKXP_005029"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO7649820.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3277..4305)
                     /gene="murB"
                     /locus_tag="ACHKXP_005030"
     CDS             complement(3277..4305)
                     /gene="murB"
                     /locus_tag="ACHKXP_005030"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO7649821.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDSVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQIGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1170 a         1154 c         1049 g         1226 t
ORIGIN      
        1 tgtcgcattt gttcaggaac acgatgatgt acggaacgcc tacctgacga cccagcagga
       61 tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg atcgcgccgt
      121 ccatctgcgc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc gggcagtcta
      181 cgtgtgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg atggtgatac
      241 cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca gcaccgccgt
      301 aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca tggtcaacgt
      361 ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct ttagacatcg
      421 attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag gcaacatgcc
      481 cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga agtggtgctg
      541 ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tgcgctctac caactgagct
      601 atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag cttggaaggc
      661 tgaggtaata gccattatac gatgcccgca tcctggaact cggctacctg attttcattc
      721 tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagcgaact tatcgtctcg
      781 ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc ttcgaagtct
      841 gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc ggacttgatg
      901 gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg ctctacctac
      961 tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt catgcaacta
     1021 aaaaattgca taatttgttt tattggtcac attttatgcg acacgatgaa gaaacagccg
     1081 aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg cgctcctgct
     1141 aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa cgatagcttc
     1201 ctggcagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct gcccattaac
     1261 ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag agctatgacc
     1321 gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta cctacagttt
     1381 gaccgcaacc agtgggcagc tctgcgtgat tccgtaccta tgacgttatc ggaagatgag
     1441 atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc cgagatctat
     1501 ttacctttgt cacgtttgct gaacttctat ataagctcga atctgcgccg tcaggcagtt
     1561 ctggaacagt ttcttggtac caacgggcaa cgcattcctt acattatcag tattgctggc
     1621 agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt aagccgttgg
     1681 ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc taatcaggtt
     1741 ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga tatgcatcgc
     1801 ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc acctgtttac
     1861 tcacatctta tttatgatgt gatcccggat ggagataaaa cggttgttca gcctgatatt
     1921 ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca cgatccacat
     1981 catgtatttg tttctgattt tgtcgatttt tcgatatatg ttgatgcacc ggaagactta
     2041 cttcagacat ggtatatcaa ccgttttctg aaattccgcg aaggggcttt taccgacccg
     2101 gattcctatt ttcataacta cgcgaaatta actaaagaag aagcgattaa gactgccatg
     2161 acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc tactcgtgag
     2221 cgcgccagtt taatcctgac gaaaagtgct aatcatgcgg tagaagaggt cagactacgc
     2281 aaataatttg caggggagcg aatactcccc tttcttattt ttctgcacta cgcagggata
     2341 tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa gccccctgtt
     2401 tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt ttcactgggc
     2461 gattaataaa attatccagc ttttcccagc gcgacagata aggtgccaat ccttcttgtt
     2521 cgaagagttc caacgcagca cgtaattcac gtattagcat ggccgccaac gtattacgat
     2581 cgagattgat ccccgcttcc tgcagcgtga tccacccctg attaacgaca ctctcttcaa
     2641 cacggcgcat tgccatgttg atcccggctc caatgactat ttgcgccgca tcgccagttt
     2701 tgccagtcag ctccacaaga atgcctgcca gcttgcgatc ctgcagatag aggtcattag
     2761 gccatttaac acgaacttta tctgcaccca gcttgcgtaa tacttccgcc atcacgatac
     2821 cgataaccag acttaaacca atcgccgccg ccgggccttg ttccagacgc cagaacatcg
     2881 acaaatataa gtttgcgcca aaaggcgaaa accatttccg accccggcga ccacggccag
     2941 cctgctggta ttctgcaatg caagcatcgc ccgatttaag ctctccgata cgatcaagaa
     3001 ggtactgatt cgtggagtca atcactggca gcacggctac actaccgcca tccagctgac
     3061 ccaatatctg tttagcatta agtaactgga taggctcagg caggctgtat cctttacccg
     3121 gaacggtaaa gacatcaacg ccccagtcac gcagtgtctg aatgtgttta ttaatagccg
     3181 cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca ccgttcgcta
     3241 acagggcaat caatttcagt ggcacggtgt tatccttcat gaaattgtct ccactgcgct
     3301 cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat taaatttttc
     3361 accaactttc tgtcttacat gatgcgccag ctgtacaaca tcttcgcttt ttgcattgtc
     3421 ttcattaatg agaactaacg cctgttgacg gtgcaccgca gccccaccta tttgcatccc
     3481 ttttagctgg cactgatcga taagccaacc tgctgccagt tttactgaac catccgcctg
     3541 ggggtaattt ggtgctgttg gaaattgtga cagtaatgct ttagccgttt cggcagatac
     3601 aacagggttt ttgaagaaac taccggcatt gccattcact tttggatcag ggagtttggt
     3661 ggtgcgcata tgacacaccg cattaaatac ttgctgtggc gttactgttg taggatccag
     3721 acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac gcagacctac
     3781 ggctacaata gcgaagcggt cctggtattc atgtttaaaa atactgtcgc gatagccaaa
     3841 acggcactct ttggcagtta agcgcacttg cttgcctgtc gccagttcaa cagaatcaac
     3901 ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga taggtgatga
     3961 gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt cctgcaaagt
     4021 gtattttacc agacgatgcc agttttctcc ggctcctaca tgtaaatacc acgcatcagg
     4081 ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc gatagtcctc
     4141 cagaaaaagt acattacttc cttcacccag aataagaacg ggttgtcctt ctgcggttgc
     4201 atactgccag gcattgagta attgttgttc gtcttcggca catacaatgt gctgagcatt
     4261 atgatcaatg ccaaatgtgt tccagggttt taaggagtgg ttcatagctg ctttcctgat
     4321 gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagata tgacgacagg
     4381 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg
     4441 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt
     4501 caaatccgct cccggcggat ttgtcctggt tgggagagcg ttcaccgaca aacaacagat
     4561 aaaacgaaag gcccagtctt tcgactgagc ctttcgttt
//
LOCUS       DAZFZC010000210         4538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFZC010000210 DAZFZC010000000
VERSION     DAZFZC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.214"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(132..734)
                     /gene="fimB"
                     /locus_tag="ACHKXP_005031"
     CDS             complement(132..734)
                     /gene="fimB"
                     /locus_tag="ACHKXP_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO7649822.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2188..2904
                     /gene="nanC"
                     /locus_tag="ACHKXP_005032"
     CDS             2188..2904
                     /gene="nanC"
                     /locus_tag="ACHKXP_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO7649823.1"
                     /translation="MKKAKILSSVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2924..4030
                     /gene="nanM"
                     /locus_tag="ACHKXP_005033"
     CDS             2924..4030
                     /gene="nanM"
                     /locus_tag="ACHKXP_005033"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO7649824.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKRWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWYNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVMDSVLISVKDNKVTVQN"
     gene            4095..4445
                     /locus_tag="ACHKXP_005034"
                     /pseudo
     CDS             4095..4445
                     /locus_tag="ACHKXP_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            complement(4463..>4538)
                     /locus_tag="ACHKXP_005035"
     CDS             complement(4463..>4538)
                     /locus_tag="ACHKXP_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7649825.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1395 a          927 c          930 g         1286 t
ORIGIN      
        1 ttaacttaat gtttattatt ataattaaga atcacactta caataacatc aattaataaa
       61 tggttaatca tttttgatat atcgtaagaa taatgtagtt tttaacacca tccctggtat
      121 ctcaactatc tctataaaac agcgtgacgc tgtcgtcctc tggccctatc ccagatgccg
      181 taaaaacgcc ctgcattgct ggcggtatac cagacagtat gacgaatatt gcgatgccca
      241 agataatcct ggataagtcg cgtatctatt cccatattcg ccaaagcaaa accacacgaa
      301 tggcgtaaca tgtgcggatg aatctccagt gacaacccgg cattaccacc ggaagtcgag
      361 ataatatggt aaaactgttg ccgagaaagc ggattcccct tacgcgataa aaatacccat
      421 tcgctctcag catgcgggta cgaagtacgg atactcaacc agttttttaa agcctgaact
      481 tctttgttca atagcgggtg cgttgttgaa aagccttttt ttaatcgatg gatatagata
      541 cactttgcct taagatcaat atccgaaatc ctcaatcgac aaatttcact cgcccggaaa
      601 ccatgaataa aacaaagcaa agtcagacaa taattacgtg ctgcatgagg cccggtattt
      661 gctgctttaa ggagtgattc gatttcacta tgggtcagga agttcctttt tttgttatca
      721 gccttattct tcatcgtttt cccttataat tacagacgcg cactagctgt gctgggttag
      781 caataatcca acattttaat cctgattatg tttataaaag cgaacgtttg cttaataact
      841 aacgtaagtg gaccagttct tctgagtgaa cttaaatgga gtagcgatag ttacttataa
      901 taactttgcc atacaaacgc catgctcttg catgctatgt acctttatat atttataaat
      961 ctgatacgaa tcgaataata accctcatca ttaatgcaac taatcttatc tatatatcat
     1021 gtgatatgtt ctgtaacaag taaaatcaac ataaaaaatt cagcaaaatt taatattcgt
     1081 tcaactagcc gtctcacata tgacattatt agtcaactgc atttgccaca aaatggctat
     1141 ttcatggaac gcagtaattt tttagtggct attatcatgc taattcttag atccctagcg
     1201 attattcctg cctggttcgt cagcttaggc catgagattg ttacattccc ttttatcctg
     1261 gagatatggg ggaacgcagg cgacgctttt tcatctacat ccgttggtaa ctatggcttt
     1321 ggtcactacg acttaaacct tcctcaatca cagctattcg ccgggcgata cggcaaacaa
     1381 actaagtgaa acatcatcct gaaaacacca acatcaacaa gcctctccag atcgacatca
     1441 ggagtgacca gttacaagcc acaaaccaag aaacatattt catgatgaga attatactca
     1501 gcctgatggc gggtaaacac ttattttcac caagctcact gagttagctt gcaaagcttt
     1561 ctttgtttag attctaaata aattatttac aaaacagatt gttaaaagaa aacctttagt
     1621 ttttgaagac caatgcaagt gttcgtctgt cttcacatgg catctgcttc ttaggaaaag
     1681 atcgacatat tttgtgacac gaattgcaaa tctggttttg ttgtatggat tgcgtgattt
     1741 ttgatctggt ataacaggta taaaggtgca ccgcgatagt caatgagact gggcatctcg
     1801 taatctatgg caaacatcac ttcagttctt tctcatcggg tgatgaaaac gcacttcagt
     1861 ctgaaaggaa tatgaaaatg agatcaacag acactctatt ttatgactct gggtaaaatg
     1921 gattgagtaa gcgatatagc ttacgaaaat tcaaatcaat taaacatcag cagagatttt
     1981 atactcaggt atttaatctg gccctctgtt tatttaaata atgtggaaag agatttttca
     2041 caggagatat tatacaaaaa atataaaata cagctaccgg ttgccaaaga cactataagt
     2101 ctggcaaaaa aatattacac aacataaatg ctaattgttt atgcgggctt tgtattgctt
     2161 tctgtatcct acaaatgagt gaaatttatg aaaaaggcta aaatactttc tagcgtatta
     2221 ttactgtgct tttcgtcccc attaatttct caggctgcga cactggacgt acgtggtgga
     2281 tatcgtagtg gaagccacgc ctatgagact cgactcaaag tcagtgaggg atggcaaaat
     2341 ggatggtggg caagcatgga aagtaatacc tggaatacca ttcatgataa taaaaaggaa
     2401 aatgccgcac tcaatgatgt tcaggttgaa gttaattacg cgattaaact tgatgatcaa
     2461 tggacggtgc gcccgggaat gttaacgcat tttagcagca atggcacacg ctacggaccc
     2521 tacgtaaaac tgtcctggga cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac
     2581 gactggaaag cttaccgaca acaagactta tctggtgata tgtctcgtga taacgttcat
     2641 cgttgggatg gatatgtcac ttaccatatt aatagtgatt tcaccttcgc atggcaaacg
     2701 acgctataca gtaaacagaa cgattatcgc tatgcaaacc ataagaaatg ggcgacggaa
     2761 aatgcatttg ttctacaata ccatatgacg cccgatatta cgccatacat agaatatgac
     2821 taccttgacc gtcagggtgt ttacaacggc agagataatt tatcggaaaa cagttatcgc
     2881 attggtgtgt catttaaact gtagtagaca ggagacagtc acaatgaata aaacaataac
     2941 ggcgcttact atcataatgg cttcatttgc cgcaaacgcg tctgtattac cggaaactcc
     3001 tgtgccattt aaaagtggta ccggagcaat tgataacgac actgtctaca ttggtttagg
     3061 tagcgcaggt acggcatggt acaagctgga tacacaggcc aaagataaaa gatggacagc
     3121 gttagctgca ttccctggcg gaccgagaga tcaagcaacc tctgcattta ttgatggcaa
     3181 tctgtatgtg tttggcggca ttggcaaaaa cagcgagggc ttgactcagg tatttaatga
     3241 cgtacacaaa tacaacccca aaaccaatag ctgggttaaa ttgatgtcgc acgcgccgat
     3301 gggcatggcg ggccatgtga cttttgtaca caacggcaag gcttatgtta ctggcggtgt
     3361 taaccagaat atcttcaatg gctattttga agatctcaac gaagctggaa aagattcaac
     3421 cgctatagat aaaatcaacg cctactattt tgacaaaaaa gcagaagatt atttcttcaa
     3481 taagtttctg ttgtcttttg atccctcaac acagcaatgg agttacgctg gcgaatctcc
     3541 ctggtacgga acggctggtg cggcggttgt gaataaaggt gataaaacct ggcttattaa
     3601 tggcgaagcc aaaccaggat tgcgaacgga tgccgtattt gaacttgact tcaccggtga
     3661 taatttaaaa tggaataagc ttgatcccgt ctcatcacca gatggcgtcg ctggcggttt
     3721 tgcggggata agcaatgatt ctcttatatt tgctggaggg gccggattca aaggttcacg
     3781 agaaaattac cagaacggta agaactatgc gcatgaaggc ctgaaaaaat catatagcgc
     3841 tgatattcat ctttggtata acgggaaatg ggataaatcg ggtgaattat cgcaaggtcg
     3901 ggcctacgga gtatcattgc cctggaataa tagtttatta attattggcg gtgaaactgc
     3961 aggcggcaaa gcagtgatgg attcagtttt aatttctgtg aaggataata aagtcacagt
     4021 acaaaactaa cgcttcaggg ccccggtaaa ggggccctgc tatcaatttg tcatttcaat
     4081 tacgcgaaat ttatatgaac gcaataatat cgcctgatta ttactatatt cttaccgttg
     4141 ctggtcagtc taatgccatg gcgtatggcg aaggactgcc attaccggac agggaagatg
     4201 cgcctcatcc cagaattaaa caattagcga gatttgcgca tacgcatccc ggaggcccgt
     4261 catgtcactt taacgacatt attccactga ctcactgccc acacgatgtt caggatatgc
     4321 agggttatca ccatcctctg gcaacgaatc atcaaacaca gtacggcacc gttggccagg
     4381 cactgcatat agcacggaaa ttactgccct ttattcctga taatgcaggg gttctcatcg
     4441 ttccgtgggt aatgactcca acttactgat agtgttttat gttcagataa tgcccgatga
     4501 ccttgtcatg caactccacc gattttgaga acgacagt
//
LOCUS       DAZFZC010000211         4377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFZC010000211 DAZFZC010000000
VERSION     DAZFZC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.215"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..942)
                     /locus_tag="ACHKXP_005036"
     CDS             complement(<1..942)
                     /locus_tag="ACHKXP_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649826.1"
                     /translation="MRTFTNLLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQERRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILKNLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEIFYKFENSAEGTLSRYSVSLDD"
     gene            1043..1330
                     /locus_tag="ACHKXP_005037"
     CDS             1043..1330
                     /locus_tag="ACHKXP_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7649827.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKRKELNDDDKTITP"
     gene            1302..2831
                     /locus_tag="ACHKXP_005038"
     CDS             1302..2831
                     /locus_tag="ACHKXP_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO7649828.1"
                     /translation="MMTTKLSLPELDTTKLPDRYQALLDEMHEETGIRREILLSVMLT
                     VKAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAAILELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(3001..4218)
                     /locus_tag="ACHKXP_005039"
     CDS             complement(3001..4218)
                     /locus_tag="ACHKXP_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7649829.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARAARIMREQLAAGDDPLEIKKAEAEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPHQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRIWTIPAERMKARI
                     QHRVPLSRQAIHILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAISDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         1319 a         1008 c          854 g         1196 t
ORIGIN      
        1 atcatcaagt gaaacactat aacgagacaa tgttccttct gcactgtttt caaacttata
       61 aaatatttcc agtaattttt ttgcataagg cgttgtatca aagctggtaa agtcataaag
      121 atattgcgca gcgtaaccat acaaaccatg aatccagagt ccttctctca ccaacactct
      181 gagataatat gcaaggtttt taagtatctt cgtctttcgt tgtatatcaa gtaagacact
      241 taatgaataa aatgcattat agaatttatc aatattcctg agcactttct ccagttcatc
      301 cttatccagc ttcttctccc ggtaatcctt tttatattca tttttgatag ccatgatata
      361 ttgttgcatc aggctcttgc ttttcccgtt atccttccgt atagttcctg tatatttccc
      421 cagagcatac agctttccat tttcatgaaa atcttccttg ttgaatccca gttcagccag
      481 atattcccga agataacgtt taacagcagc ttcatcgctt tctgtatcag cgtgatcaag
      541 acaaggacaa tacgatttct gtaaaaaatt ataaatatga taataaaaag cagagtcatt
      601 ctctggtctg tatgtattat acatatctac aacaagaaaa ataaccccaa aagtgcgcag
      661 acaatagctg cgaaggaatt caggttttag tgtggaatgc actggataag aattcatcat
      721 gtaataaaaa tgttcatatc gggcatatat gatccgccgt cgctcctgac tgaatacccc
      781 ttcagcatca ataatacgac tgatctcatt ataacgttga tcccagaatt tttgatgcat
      841 ttcaaaattg gtactcttcc gcttgtgtgc cggattctcg ccttccttaa acaaccccaa
      901 aacagtacaa atatcataca acagatttgt aaatgtgcgc atatgctgtg ctctccggtt
      961 ggtttgcatt ttatcgcaga ggacattctg agcttaacgt atcaaactga cacaacgaca
     1021 tgcaatgcat atggagaaca atatgtcaaa tcaaaccgga cagctttaca cgccttcacg
     1081 catcctgcgt atggctgaac ttacccgact gctgggtata tcaagatcta ctatttacgt
     1141 gaaaataaat ccggcctcga aataccacga ccctaccttt cccaaaccaa taaggcttgg
     1201 tagtgcatcc gtaggctggc gggcatctgc catcgatgag tggctgatgt tacatacggc
     1261 accagcctgg tctgaacctg aaaacaaacg aaaggaactt aatgatgacg acaaaactat
     1321 cactccctga acttgacaca actaaactgc ctgacaggta ccaggcatta cttgatgaaa
     1381 tgcatgaaga aacaggaata cgccgtgaaa tacttctgtc tgttatgctg acggtcaagg
     1441 ctgcctcggt ccaggatacg catgaagttg aactttccgg agggcaacgt acaagtcttc
     1501 agatatttat gtgtctttca tcagcatcag gcagtggaaa aacctctgcc tgcgcaaaat
     1561 taatcgctcc tgttcatgaa acagaagaag aactacatca ggcatatatc gacgataaaa
     1621 aaaactatga ccgaatgatg gaaatgtgga caacagataa aaaaatcctg gagcggagat
     1681 ataaaaagga aattgaaaga tccccggaaa atgctgctgc tgcacgagca gcacttgaaa
     1741 agtgtatagc aaataaaccc gaacctcccg tacaacaggt tctcatcgtg aatgacgcga
     1801 caccagaagg gatagctttg aaactcagcc agtcaccctc tcttctgttg ctgtctgatg
     1861 aaggaggtac tattctggat aagcgtttcg aacgcaaatc ggcactgtac aacacattat
     1921 ggagtgggca accagtcaac gttgaacggg catccagacc cggattccgg ctgaatgatg
     1981 tccgtctcac tatacttata ctgactcaac cggtaatatt taataagttt tttactctca
     2041 ctggcgatca gatccggggc aatggctttc tggcccggtt gctattttgc gaacccggag
     2101 caattaaaat aatgacgaca gaaccgcata ctgccactcc tgttgtgacg caacaatgtg
     2161 caagctgttt tcgtaagaaa agttttggca gtcagatcag agactctctg agagcatcca
     2221 gagaaagacg tgcaaaaagt aaagagcgca tctgcatgac attatcaaat gcagcatccc
     2281 gcgcactgga gcgctttcac aaagaaaata tggatactgt cagacagaat cctcatatgg
     2341 gtacttttga agacattatt gtcagaaaaa gagagcaggt tgtcaggata gccgcaattc
     2401 tggaactgga gaaagatcca gactcgacag taataacgct ggaaagtact aatagcgcca
     2461 tttatctcat tgaattttat ttcaagcatc ttatatataa acttgaatcc cttcgggaaa
     2521 tatccccggc tgagaaactt gataaatggc ttcagaaaag aattatcaca acagcaggtt
     2581 acatattcca gaaaagttat attttacaat atgccccata tgctctcagg aaaaaatgtg
     2641 ttctggatga agcacttgac atactggcgg aacaaaggaa aataaggatt gatgataacc
     2701 tggttgttta tataggaaac acgattacac catccgaact ggcaaaaaaa ctaaatattc
     2761 ctgcattcga tgccggagtt ttcatttgtg atcatcaaaa catactgaag tatcaccgaa
     2821 acagactata gatactatcg gacaaaaaat aaattatcac aagtgtatta ttcgttataa
     2881 taatgaataa tcgttatctg agtgcaatgc caggttttca ttacgaactg gcattgcatt
     2941 tatttttcaa aaaagaaagc agtaaccatt tggctactct gagtatgcca gtccatgaaa
     3001 ctaagcgaat tccttagtgt ttttagcaag ttgagacatc acataatccg cccatgcctg
     3061 catcatcggt acacgctgct ccagtagatc agtacgatga tatgccgcct caaccttatt
     3121 tttcagcgta tgagcgagcg ccctttccgc cagatcccgc gaatacccct gttcactaca
     3181 ccagtccctg aatgttgagc gaaaaccatg tgccgtggca actcgccccg gtatatcact
     3241 gatggctttc tttttacgca gaaaactcgt caacaccata tctgaaagga tctgctgctt
     3301 tctgggagaa ggaaacacta gttcatcatg caaaccacgt atattttcca gaatgtgaat
     3361 ggcctgccgg gataaaggaa cacgatgctg tatcctggct ttcattcttt ctgcgggtat
     3421 agtccatatc cgcttatgaa aatcaatttc agcccagcgc attcccctgg cttcgcccga
     3481 gcgggttgct gtaagtatca ccattaataa cagtgcccgg gtaacattat aaggttcatc
     3541 tgtatacaca ctggtcgcca caaaaagcgg taactgcctc cagggcattg cgggttggtg
     3601 ttcatcacgt cctcttgtct gatgaggaag caaatggtca accacatcaa cagggtttgc
     3661 cacacaaaaa ccgtgcgccc atccccactg cataacaaca tgaatgcgct gtttaacccg
     3721 gcttgccgtt tctgacaagg ttaaccagac tggacgcagt gtttctgcca cgtccgcagc
     3781 cgtaatcgaa tccagcgttt ttgctcccag ttgaggaaac gcgtaatttt caagcgtcga
     3841 taaccactgc ctgacatgct ttggattttc ccatcccggt gacaattcag catgtacacg
     3901 cctggctgca tcggcaaatg ttgggataac aactttttca gcttcagcct ttttaatctc
     3961 cagtgggtca tcacctgcag caagttgctc tcgcattatc cgggcagcac gtgcagcttc
     4021 agcaatactg acctcaggat aagttcccaa tccagcatta cgcctttttt gtgtcaccgg
     4081 acttacataa cgaaaaaccc atttaccccg cccttttact gaagaaggat gaagagtcag
     4141 tcctgtaatt cccccatggg gcaatggttt gtcatcaggt ttgatatgcc ttgctttagt
     4201 atccgtcaat actgccatac gctaattcct gttactctgg tatgccatcc agtatgccat
     4261 tagtctctcg cttcactaag aatacattag ataatatcgg acagcaaaaa gtgtaataca
     4321 ttgtttataa tgaaattata taacaccccc ggattaagtc agatttattt caggcgg
//
LOCUS       DAZFZC010000212         4358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFZC010000212 DAZFZC010000000
VERSION     DAZFZC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.216"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(162..675)
                     /locus_tag="ACHKXP_005040"
                     /pseudo
     CDS             complement(162..675)
                     /locus_tag="ACHKXP_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(736..840)
                     /locus_tag="ACHKXP_005041"
                     /pseudo
     CDS             complement(736..840)
                     /locus_tag="ACHKXP_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(842..1165)
                     /locus_tag="ACHKXP_005042"
                     /pseudo
     CDS             complement(842..1165)
                     /locus_tag="ACHKXP_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1911..2888
                     /locus_tag="ACHKXP_005043"
     CDS             1911..2888
                     /locus_tag="ACHKXP_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO7649830.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3173..3913)
                     /locus_tag="ACHKXP_005044"
     CDS             complement(3173..3913)
                     /locus_tag="ACHKXP_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7649831.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGGDAAEILRTLPPLR"
     gene            complement(4034..4222)
                     /locus_tag="ACHKXP_005045"
     CDS             complement(4034..4222)
                     /locus_tag="ACHKXP_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO7649832.1"
                     /translation="MESEIFSCLLRASAGFFLRFCLSWHYPNSPKNSKPALSPHHDWL
                     KTGSQRQNAPLRIWFYPQ"
BASE COUNT         1070 a         1102 c         1056 g         1130 t
ORIGIN      
        1 tttttcggtt gaaaagaaat tgattgaatt actggaaaaa caatctggtc tccatacgtc
       61 tgtggattgc agtactcccc ttacagagca gagtctgtcg agaaatgacg gtgaagacca
      121 gaccagaaga aacgctgaaa atatgttcgg agatgactga actaacccag cctggccagg
      181 ggaatatctc gccatttagt tgtataacac tgactcaaca tttcccgttt catttgccat
      241 tcaggggcgg ttcctcgccc cgcaaaccac actttccctt ttcccgactg gttcagttca
      301 tcgagtgttt tcattaactt ttcactgttt ttacggggct ggatttcatc aaataatccc
      361 ggctgcgcta tacccgatgg tgtgaaatca gccagcatga ctcctgcctt catatagcgg
      421 tacccttcac gccagacatg gtttaaggct ctgcatgcgg cggcaataat gtcccggctg
      481 tcctgtgtgg gtaatgaaag cttttccaca gcggcattgc tgtaacaggg ttcaggggga
      541 tgtccgtaca aatgtcgtca cctgccggca atactgacgc tccccacgta gtttctctgc
      601 ggcccgctct gcatactgaa caacagcctg gtgcatggca tctttgtctg tgattcgttc
      661 accaaaactg cgactatgtc aacgccacga tgtttgaccg ttatttgcca ttttcatgcc
      721 cgctaaatac tttcgttact gatgctgtcc gctttgcacc atttcatcat cactgattgg
      781 cgttgtgttt ttggtcagca ctccagcttt acgtttctct tttaaccggt aactctctcc
      841 ttgttctttt tgacggacgc attttacgtt tgggctggat gtagtaacgc agcatggaac
      901 gtccgcccgt ataacccatt gccttaatct ccgcgaagat aacctcgcta ttccagacat
      961 tctctgccag gcgcatgtcg atgtaatcca taaacggttt cagcttaacc attttgtggc
     1021 gggtctttct ggctggcggt tcagggtatt tgaggtagcg tctgaccgtc cgttcagagc
     1081 aaccaatctg agccgcaata tcgacaatgt acgcgccctg ctggcgcatt tgctttatca
     1141 tgtaaaagtc ctctctgctc agcatgttga tgtcctttct ggtgtgagaa ccttaaggaa
     1201 acaacatgtt gggtggagcg gacaatccaa atggtgaatt accgtcttat atcactggcg
     1261 ctgacacctg tagtttgtat acacaatccc tggcattgaa acacacatat gaagtgtact
     1321 tcggatatga ccgtacgctc aggaagtacg gcagattccg tcaaaaatta cctgtaaaga
     1381 cagacatcag aaaaaacagt gctcgccgca gaccggtgac cgggcgaagc ccgcgaaccg
     1441 gcgaggaagc gcaatgtatc tgtagttaca gggagcaggt acgccctgaa gccctttcat
     1501 aaaacggttg tgtgcgctgt ccttctggaa tgatttatcc acatatccgg aggcccgatc
     1561 cagtaaacag atccatgaat gatcaacaaa ggatccatta aagatcccca tatcgctgca
     1621 aaccttgtca ctcatgggcc gggaccacga tcacataagc tgtggcatgt tactgataaa
     1681 ctgtaacatg ctaatgataa gctgtattca gtaatccata tactgaagta agttaatgac
     1741 ataaactatg gtcagtatgc cagactcagt tgttaaatac aggctgcagg tttttcttca
     1801 gtcagttagc ggagttctga cacacgattt tctgtttatt cttttactgt ccacaggctg
     1861 gaggctttct ggaaaacgaa aattcagaca tcaaaaaact gttcggcgag gtggataagt
     1921 cctccggtga gctggtgaca ctgacaccaa acaataacaa caccgtacaa cctgtggcgc
     1981 tgatgcgtct gggcgttttt gtaccgaccc ttaaatcact gaagaacagt aaaaaaaata
     2041 cactgtcacg tactgatgcc acggaagagc tgacacgtct ttccctggcc cgtgctgagg
     2101 gattcgataa ggttgagatc accggccccc gcctggatat ggataatgat ttcaagacct
     2161 gggtggggat cattcattcc tttgcccgcc ataacgtgat tggtgacaaa gttgaactgc
     2221 cttttgttga gtttgcaaaa ctgtgtggta taccttcaag ccagtcatcc cgcaggctgc
     2281 gtgagcgcat cagcccttcc ctgaagcgca ttgccggtac cgtgatttcg ttttcccgta
     2341 ccgatgagaa gcacacccgg gaatatatca cccatctggt acagtcagct tactacgata
     2401 ctgagcggga tattgtgcag ttacaggccg atccccgcct gtttgaactg taccagtttg
     2461 acagaaaagt ccttctccag cttaaggcga ttaatgccct gaagcgacgg gagtccgccc
     2521 aggcactcta cacctttata gagagcctgc cccgggatcc ggcaccgata tcgctggcgc
     2581 ggctgcgtgc acgcctcaat ctgaagtctc ctgtattttc ccagaaccag acggtcagac
     2641 gggcaatgga gcagttgcgc gagattggat atcttgatta cacggagatc cagcgggggc
     2701 ggacaaaatt cttctgtatt cactaccggc gtccccggtt aaaagcgccg aatgacgaga
     2761 gtaaggaaaa tccgttgcaa ccttcacctg cggaaaaagt cagtccggag atggcggaga
     2821 agcttgccct gcttgagaaa ctgggcatca cgctggatga cctggaaaaa ctcttcaaat
     2881 cccgctgaac ataaactgta gtcagtgaag agcgctcctt tactgactac agcttatatt
     2941 aaccggtgca gtgagtggtc tgctcactgc agtttatatt ctgtttcctg cagtgctgtc
     3001 tgtagctgag ctgccatctg cctgtccctt acgtaagtga tcccgtaacc tgatgctgag
     3061 gcattgctcc cttcataaaa catgacttac tcactacagc ttatatacat gctccagctt
     3121 atgttatgtc tgttctgctg accacagctt atataaggaa gcgcgtatgc cgtcacctca
     3181 ggggaggcag tgtacgcagg atctccgcag catccccccc gtcacctgtg aaaggcactg
     3241 ccagcgtggc agccatatcc agtgcaaaca ctctggtata aacctccatc gaacgtggat
     3301 ccctgtgacc agccagtgcc tggatgactt tccggggctg gcggtgatag agcatgtgca
     3361 tgatatagct gtgccggaag gtgtgtggtg tgaccggaat cgaaaagtgt actccgtcag
     3421 cttcggcccg tctgacagcc tgcttcagcc agttgcgcat ggtttcgtcg gtcacggccc
     3481 ataatggttc acgacgacgg ggccgggtgg tgatcatcca gctttccatc tgcctgacat
     3541 agcttatatc tgtcagcgga accaggcgca cttcatcttt tggcgggcgt ccgcgtcgcg
     3601 cacgcacttt ttcggacagg atccgcacaa acggtcttac tccattcagg tcaaatgatt
     3661 ccggcgtcag catccgggct tcgccaatac gcattccggt attccagagg gtggcgaaca
     3721 gcatatggtg acgctgatcc ggcatataga aaagaagggc actcacttcc ggggccagca
     3781 ggtaggccgg tgtggccccc gtggaagtgg ccattcttct cagggagagc gctgttgcaa
     3841 aatccacccc cggcgcaatg ggtaacaggg agacgcgttc tggtgaattc tgcaggggaa
     3901 tgacattgtt catgagttat ttttcacgta gcacacgcac tgtttatgga tacaggtatt
     3961 ctgacctgaa cgcccggatc cttccacgac aataaatcgc agcgcgcgga tccttttgtt
     4021 tgattatcac gcattattgc gggtaaaacc aaatacgtaa aggcgcattt tgccgctggg
     4081 atcccgtctt aagccagtca tggtgcgggc tgagggctgg tttactgttt ttgggagaat
     4141 tggggtaatg ccaggacagg cagaaacgga gaaaaaatcc tgcagacgcc cttaaaagac
     4201 aactgaaaat ttctgactcc atattcctct ggtttcagac tgcttaatca catcatcaac
     4261 ctgatacata acaaacagat tctgttcaca gaatgaacaa ttaagtaaat ggttagatta
     4321 tgcgaaaaag tacagatatt tctcaccatc gttattga
//
LOCUS       DAZFZC010000213         4320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFZC010000213 DAZFZC010000000
VERSION     DAZFZC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.217"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(441..3122)
                     /locus_tag="ACHKXP_005046"
     CDS             complement(441..3122)
                     /locus_tag="ACHKXP_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO7649833.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAERVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSVFTEHAVFNYLCRVANERIFYVRGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLMIPERRLALFTSLQLS
                     YYEKTIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAET
                     YRDEFDRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEVFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(3364..3543)
                     /locus_tag="ACHKXP_005047"
                     /pseudo
     CDS             complement(3364..3543)
                     /locus_tag="ACHKXP_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
BASE COUNT         1230 a         1017 c          840 g         1233 t
ORIGIN      
        1 tggggataaa taataaaaat aaaactcgtc agatattaat ctggttgttg gatttgttat
       61 cgcatccttt atccacctgc ttcttactgg taacacatct ggaaaatcca tgcattttca
      121 atatatacta tcctttcaac ttgtaaataa ggaaatatga tgcagatggt gaacgattca
      181 aatatcattt tgcaggaagt attcactgta atagaatccc ccctgataaa gtttattcac
      241 taaggaatta ttcaacagaa ccattccctg ttagctttga gtgctttgag caatagcact
      301 tatttttata tgtatcagga atacgtacgt tgatgtaacc aaaacaaaga agcctgtgtc
      361 catggggttc gtacaattgt gtgtggcaca tgaccaaatc aggtttgaga cctcccgctg
      421 caatattttt tattcagact ttagcctgtc aggcctgaca gaaacgctcc caacttccct
      481 tctatatcac tgtctatagt acgtaatctt agcaatggca ggccgcattt cttcagtata
      541 ctgtttttaa gttcatcacg ttcagcctga acactggtca gatgataacc tccatccact
      601 tcaataaccc ctaatggagt cttcccgacc ttatagtaaa caacaaagtc acaactggcc
      661 cggttacgca tgaataactg ttcacggtgt gtaaaatccc cccgctccag caacaccaac
      721 tgattcagcg ctatttgtga atgaaacatc atactccgat aactatcctg cgacaggata
      781 tcccgcaaca ggcaggccac aatctgttct gacttaaagt gtgaatcatt tgaattcaat
      841 ctactattca gtcgttccag tgatttgtca tactcactgt acagcaaatc aaatgctgat
      901 atgaccgggc tctcgaaaac ctctccgtca tcagcgtagt atttgatgta acgaatcagc
      961 gccgcaatat gtccagcgtg gcgttcaaat acatcattac cagtcaccag tgtaaattta
     1021 tttcgggccc ttgaaacagc gacattcacc agttcaggat tatcaacaaa cgcgatgttc
     1081 cgactgtgct ggctgctgcg cttcttatcc agcacagtgg aaaacacaat ttcatcgcac
     1141 tcccgtccct gaaatttatg caccgtcgat ttcacaaaat cagcaggcag cactttttca
     1201 gccagattaa cctgtgcatt atatggggca atgtatcccc tgctactttc ttcatcccag
     1261 taatgacctt ccagagattc aagctccctg agatttgaaa aattacgggt atggtttcct
     1321 tttgccgtga tgaccagaga taatgaggct tcaccggaat caaccgttaa cggtatcaga
     1381 gcattatcat aaaactgctt attacaaaat tgaatgattt taggatgaca acgataatgc
     1441 tctttaagaa gggttactgg caccatgttc ctgaacaaca tacaaaccga atcaagtaga
     1501 ctatatttct cacaattata gtattctgcc ggtggagatg ggctgttcgg gagtaataca
     1561 ggaacatgtg gaagttgctt acgatcccct acaacgatta cattacgggc acatcccagt
     1621 cccagaatgc ccggaacaat atcctgctgc gaggcttcat caataataac ataatcaaga
     1681 agggccccct tgccgataga gttaataatc gaatgcgtac tgctaccaat aaccggaaaa
     1741 cgctttataa aacggtcaaa ttcatctctg tatgtctctg ccgagaatga acttcttgtt
     1801 gaaacatttc ttcgcagatg ttgctttaaa taaagcatag accatgatgt caggcgcccc
     1861 agtaaaattt taaaatcaga ttttttaaat acttcctcat attcattcag agaaatttgt
     1921 ttttcccgaa tagttttttc ataataagaa agctgaagag aagtaaatag tgccagcctt
     1981 ctctccggaa tcatcaatgg tttgactttc agtatcctga agtttaacaa caaatcaatt
     2041 ctgtttctga atccaatccg cctgtcagaa aggtaatgaa tataagccat caggtcggtt
     2101 gttttttgtg aggaaaatcg gtatttgtga gaaaaaagct cctccggtcg aagattctga
     2161 ctctgccatt gctgcagata tttatactca atattcagct cattaatttc tgcttttaac
     2221 gaagcgacct ggttaatggc attcaggtgt tttttcactt gttgaagtac atcatcaatc
     2281 gcatttgcag acggtgagtc aggtaagact tcctcagaac gcgaaatcga cgttgaaaaa
     2341 aactgttgcc tgttctccgt actccccagt cgtgccacca catatcccag ttgctgtttg
     2401 tccatctttt cataaacatt actgacagct gagttattat tggacaaaat tgccactgtt
     2461 ttattctgaa ttaatatatt agcaacaata ttgagtatag tctgtgtttt acctgttcca
     2521 ggtggtcctt caataactga aatctgtgag gaaaatgccc gttcgacagc cagcaactga
     2581 ctttcattaa gaccaaacgg aaaaatcaaa ccccatggcg aatcacgttt tttactctgt
     2641 cctgaacagt atgcatgcaa ggcggtatcc ggatgaggaa ctattttctt tatctgccta
     2701 aggatattct catcgatatt tctgttatta ccacggacat aaaaaatacg ttcattagcc
     2761 accctacaca agtaattaaa cacggcatgc tctgtaaata ctgaacactg ttgaaagtca
     2821 aggccggtcg ttttcatgat gtagggtcgt tcaccctcgc ggtaataaac agcagcatat
     2881 ttatcaccat atataataac ccgttcagcc cgggatgtta cagcgttccc tttcctttca
     2941 aggaaaacat ttcgcaaatt tacagttggc gttatctgac atacagaaag cggacgagta
     3001 tattttctcc cgctgcgaaa atgacaggtc agcataacct ctctgtcgtc atttccagcc
     3061 cacagcgtcc agtcggtaat tttatgagtc ttattttcac cgtcaacaca aatcgtgacc
     3121 atattctcac tcagaaatta tcatttaact tacggaaaat tgttaaggca ctcccggata
     3181 ttagctccgg aggattttcc acaactccgg gactgttaac atatttctgc ctgccatata
     3241 tacattgaat ttagtcataa cagtcaatac cagaattaca ggaatgcgat gccgatacgc
     3301 tggttattga atactggtag cccctgagca ctaattcatc acgatactct gtatttactg
     3361 ttttcactgc tttttacaac cattccccca ggtaaaatgc tgctcctccg gtgatggctg
     3421 tgtcagaaac tgccatcccg gaatagccac gctgaccacc agcaatgccg cacagacacc
     3481 agccacaaac ggtttcagaa acgtgggcgg ggatttctga agggagggtg tgggctctgc
     3541 ttttgtttca gaaacaacat acaccagacg ggagtgtggc agggtctgat attcctgtac
     3601 gtccggagca tcagaccgga gtgcctgagc cggaagggtt accggagacg atacttcacc
     3661 ctgcacaggc tgtttctcca ggcgggtgat atatcccttc accatggctt ccggtaaagc
     3721 cactttactg ctctgtttta gttcgtgccg ggccagtgtg tcactcagtg ccttcaggaa
     3781 tgtttcggtc tgatacagca ggggcaggtt gtcacggtcg tccggtggca tggcccggag
     3841 cgttttttgc agacggttga acagactggt aataatttcc agccgggcgt gtgtattcag
     3901 cggccacatc actgaccagt gatgccgggt cagcgccaca atcagagcca gcccttcatt
     3961 caggccactt atccgctgcc tgaagagata aaggcgatag taaaacagac atcagtgtca
     4021 gtgacagcaa ggacggacag cgccggaata agctttgttc ctcagccctc tcgttcctgc
     4081 aacataattt tccagtcaaa cgaaaaacct tttattcatg ccataccaac aatcaccagc
     4141 ctcctgacga tacctcccac catgttttgc agtggtacgc taccgccctt ccggtattta
     4201 cccacaccaa ccggtaccag aatgctaagt aacaaatccg ttcagaattg acttaatttt
     4261 gatcattata gaataatgat ctgaactgca gcaatatgac agatttatcg atcgatataa
//
LOCUS       DAZFZC010000214         4262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFZC010000214 DAZFZC010000000
VERSION     DAZFZC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.218"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..277)
                     /locus_tag="ACHKXP_005048"
     CDS             complement(<1..277)
                     /locus_tag="ACHKXP_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO7649834.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCSLVHENNRSWTLMF"
     gene            complement(283..504)
                     /gene="escF"
                     /locus_tag="ACHKXP_005049"
     CDS             complement(283..504)
                     /gene="escF"
                     /locus_tag="ACHKXP_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO7649835.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            complement(541..948)
                     /gene="cesD2"
                     /locus_tag="ACHKXP_005050"
     CDS             complement(541..948)
                     /gene="cesD2"
                     /locus_tag="ACHKXP_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO7649836.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            complement(955..1899)
                     /gene="espB"
                     /locus_tag="ACHKXP_005051"
     CDS             complement(955..1899)
                     /gene="espB"
                     /locus_tag="ACHKXP_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO7649837.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            complement(1920..3062)
                     /locus_tag="ACHKXP_005052"
     CDS             complement(1920..3062)
                     /locus_tag="ACHKXP_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO7649838.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            complement(3075..3653)
                     /gene="espA"
                     /locus_tag="ACHKXP_005053"
     CDS             complement(3075..3653)
                     /gene="espA"
                     /locus_tag="ACHKXP_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO7649839.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            complement(3711..>4262)
                     /locus_tag="ACHKXP_005054"
     CDS             complement(3711..>4262)
                     /locus_tag="ACHKXP_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO7649840.1"
                     /translation="SWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDL
                     PEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISIN
                     KDKDVILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFI
                     DREQRDNTLLMIGKVIDYKEDVI"
BASE COUNT         1202 a          992 c          791 g         1277 t
ORIGIN      
        1 aaaacattag tgtccagctt ctattattct cgtggaccag gctgcataac aactggcttg
       61 tctcatcatc cataaagtcc tccagagcct ttaccgcatc attttttcta tttaatccat
      121 ataaaataat aaattctaaa tgtgcgatat cttttttatc gggtataatt tgcggcaata
      181 cattcaggat agattctgca tatttgggaa gcttatgatt tgccgcagct acagccgccc
      241 acaccagtat cttgttggca gaaacatcat taaccataaa aattaaaagc tacggttgga
      301 aatggttgag accaaatctt ttatcgtttt cagcattcca ctttctacgt taacataagc
      361 agaatattgc tgaaccgcga actgcagctc taacattttt tcagggtcat taaccaaatc
      421 ggtgctatta agtaactctg gcacagaatc actcagcgtt ttacctactt cacccatttg
      481 ttgagtaatt tcagataaat tcattaaatt ttcctcaata gttaataaaa ataaaaattt
      541 ctaactattt acattcatta cgaaccaaat tccgtcttct gtttcttgct gcacagcgcc
      601 ctttctgatg aagtaattaa acaactcttc gcgcttttga cgttctgaat ctgaaccaat
      661 cttagatttc atcgcactga ttttcttaat acccgaaaaa tggtggtaca actcttcgag
      721 caagcgagtc agtgataaaa cagggccgtt aagccctgtt tggttacgtg cttcaaaaga
      781 gcacaaaatc agttcttgtt catcaagtct gtacgctaat gagaaaaaag aggtctcaat
      841 ccgccatccg ataaaaaagg cactgccaca aagaaactcc ttctgtattg tgtacccttt
      901 ttgttcaaga tagtgattaa gcacttcatc attaaacgta tcgaccatga tcaattaccc
      961 agctaagcga accgattgac ccatacgatt ctgaagatca gtgagatcgc gagcggctgt
     1021 tgtcatatca cgcagacggc tagcgatatc atcctgcgct ctgcgaactt cttgcttata
     1081 cagatccaaa ttaccctgct ggaagttctc cacagactta taacgctgtt cgctaatctc
     1141 atggttagat ttgactgatt cagataccgt tgttggcaat gtcttcgtgc cttcggcaag
     1201 actggtaacg gcaataaatg gtgtagagcc agtaatctta tctactgcag ccataaagcg
     1261 gcttgcccga cttgccttct gagcggcatc tgcagcgttt tcagccagct gagaagctct
     1321 ctgagcagaa tcggcaatgt catctgcaac gtcagatgta cggctggccg ctctcgttgc
     1381 tgtagtcatc gcttgctgca tcacgctgga tgcatcttca acagcatcag caacgctttc
     1441 cgttgcctta gtcaacgttt tagtcgcagt atcagaagcc gcatcaatag ccttagaaga
     1501 tgtagaggcg gttttttgag caatatcact cgcgcctttc gtagcactgt taattgctgc
     1561 aaaagagcct aagatcccca atactgatga aatagcccca ccaaccagag cggctgttgc
     1621 agcggccttt ttttcatcaa tggctttatt ctggctctca aaaacggcca gttggatctg
     1681 atagctctgc gacaactgtt gctgttggta atcctgcaat atacgcacca tcttgcccag
     1741 gagtttttga atttccagca tcagtttaga aacatcgact ttaccatcgg taagcaaaga
     1801 tgaattaata gcagatgcgg taattgcatt agagccggta gtattctccg aaacagaatt
     1861 aaccgtcatt acttgattag tataatcaat agtattcata attaaattct ctttaataat
     1921 taaactcgac cactaacaat acggctattt actcgtgctg agtcggatat taactgagag
     1981 gcattttgta aatagcttgc ctggtttcgc aactcggctg cagctttatc cagctcaacc
     2041 ttcgcactct cacccaggtt ttcactctgt cgagttaagt tttgcaccgc gtaagcagat
     2101 aactgagcca atatgctgta ttgcgcagaa tcattattca gcacagttgt cccaatagaa
     2161 cgtgcagagg tcgtaaatcc atttgccgtt gccgcaactt ttgaaacaac gttctcaaca
     2221 aatacatcgg ccagtttagt aactgttttc acgatgtttg aaccaacttt gttagcgaca
     2281 tcgccaactt tagacataag tgaagacacc ccgccaatgc cggctgtcag aatacttgca
     2341 gcaatagata ctccgccaaa gacttgcgct gctgtcttta acgcctgcgg ggccttatcg
     2401 cccattacat caactgcagt ttgcagtgcc attgctgttg cgctaacggc aacaacagcc
     2461 cagagtgccg ggttaaaaat agctgcaata gcagttaccg cgacgcccaa ccaaccaaag
     2521 acctgaccaa caattttact tttttgtgct ttctcttcgg ctttttgttg ttcttcgagc
     2581 tgctttttaa actccagcgt tttattatcc agcgccttat tttgcccatc cgtgtagatc
     2641 tcgttggagt ttttcatact cgcaactttc tgcgctgaag catccaggga taacagggtg
     2701 accatcatca tcatggcctg agggtcaacg ttattcacct gagaaagata aggatagcca
     2761 gagcttgcat tctgagtgct gcgctgtggt tcggttccct ctgttcgact cctaacttca
     2821 ccactaatac caccaaacag tttactcagg acatcctcag caacagaggg cgtcactaat
     2881 gagtggcctg ccggtggcgt tggtaaagcg gtactttctg tccaacttgc tgatggggcc
     2941 gtggatttaa ccagttgtaa atccagcgcg gaagtcactt caggctgatt aatacctgaa
     3001 gtagccgtag cagcgctggc cccagacctc atcgactgga tatcgctatt tacattaagc
     3061 atagttatct ccggttattt accaagggat attgctgaaa tagttctgta ttgcagtgac
     3121 tgaatatcag aacgtgcact cgttaatagg tttaacgtat ttgacatttg ctgtatttca
     3181 agctggctat tattcactac cgtggtcaag ttattcgatt tggccgaaat agcggccttc
     3241 accgtttgca aatcaccagc gcctaattca gcatttagat cgctaatacc acttactgta
     3301 atgtcattgc gaggatcatt tatatagtca atcacttctt gaggaagttt ggctttctta
     3361 ttcttgtcag aactactctg aacatcagca attttagcat ccacaagatt agccattttt
     3421 tgggctgtgg ttgactcctt agatgcctca ttcatatcag caaactttgc aatcgacaga
     3481 tcgctttgtg cctgatacat ataggcaaac atgagaagcg cagcctgaaa aacaccgact
     3541 ttattaaata gctgaactac ttcgtctttc gacatgctgc ctaaatcata gactgtcgat
     3601 gtcgaagtac tcgcgttcgc actagcaact gatgttgcag ttgatgtatc catgtatata
     3661 cctcttaata atctttctta gcaagaccaa aaacgattaa aaaatcaata tcaaataaca
     3721 tcctccttat aatcaattac tttaccaatc attaataatg tattatctct ctgttcccta
     3781 tcaataaaac aagcgtcagg taatgatgca aaaaagtctt tcaataggtg gaaataataa
     3841 acatgtttat cttcgacaat cgatacccga gaagggcttt ccaggagcca gtcttctgtt
     3901 aaccatacat gttcaataat agacaatact tcatttaaaa tgacatcttt atctttatta
     3961 atagagattg caataatatc tgtatagtta tcaagcgata aaaaaatgat tacccggcat
     4021 aaatcgctaa taattgaaga tgttttcacc ttatcttcaa aagaagacat ataagacaga
     4081 tcgaacgata gcgccctaat aataaccttt aagtgcttct cacgctctgg aagatcctta
     4141 atctccataa accagtcaag taaagaggct cccttttcag aatcctcatc cttggccttt
     4201 tcataaagtt ttttgatctt ctggatacct tcctgattta actcccccac ccctaaccag
     4261 ga
//
LOCUS       DAZFZC010000215         4244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFZC010000215 DAZFZC010000000
VERSION     DAZFZC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.219"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            728..1681
                     /locus_tag="ACHKXP_005055"
     CDS             728..1681
                     /locus_tag="ACHKXP_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HFO7649841.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGSFPDGERAIGYKQHFKMPYIGLTGNYRYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISDHTKNGAGIESYNFMTTAGLKYYF"
     gene            2114..3223
                     /locus_tag="ACHKXP_005056"
     CDS             2114..3223
                     /locus_tag="ACHKXP_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004149557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7649842.1"
                     /translation="MKLLLLTGATGFLGGAVLDKLLDNCNNINLLLLVRAPTPQAGLE
                     RIKENMRKFNVCEERLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGN
                     NPFIWNVNVTGTLAFARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHL
                     VEYTHSKATIEYLMRKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKF
                     MCSLDDKIDVIPVDYCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALN
                     CDPVGDRYTKVSYDILAMSRHDFKNIFGPCNERLMLKAIRLYGAFSMLNVCFSNDKLL
                     SIGMPKPPKFTDYIKYCIETTKHLSIQQQMEVDFK"
     gene            3292..4194
                     /locus_tag="ACHKXP_005057"
     CDS             3292..4194
                     /locus_tag="ACHKXP_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial resistance protein Mig-14"
                     /protein_id="HFO7649843.1"
                     /translation="MSIKNIKRIITAWKPSTFETYKKTFEKYGGSVNMHPDVVSYFMI
                     HHDWKFDFFHYEKDGDIKGSYFLCNGKQIGIMARRSYPLSSDEVLIPFSPHARCFFPD
                     KTNKLSIINKQNIINATWKIARKKQNCIIKESFSPKFEKTRRNEIQRFIRNGGEIKCI
                     SQLSDKEISSSYISLFHSRFGGTLPCYEYDNLLMFISHLRELMFGHVLFWDNKPCAID
                     IVLKSESSCNVYYDVPNGAVLNDENCMKLSPGSVLMWLNIDKARRYCQDNNKKMIYSI
                     GAFRPEWKYKLLWSVPCKVGKCLC"
BASE COUNT         1361 a          728 c          844 g         1311 t
ORIGIN      
        1 gatttacgca ctcccctcca ttacactaat aaacaccacc gacttggtat ttataaattt
       61 tctaataaaa gcatcatttt cactgaaaca aaataaatat caatcactaa gataattaac
      121 ttatctgaac aagtaatcac actcatatgt tattagtttt cttattaatc agaaaggagg
      181 gatttataaa ccagcaactt tttttgtagg tggagccacg gttgatttct gatatcaacc
      241 acctgagttt attttcgtcg aatggtaatg tcttgtcgca caaacgctat ctgaataatt
      301 aactgtaatg gcgttattgg tatatttgcc gacattaacg ctaataaaga ccctgattgc
      361 ttcataatcg caggatttgg ccattttgtc tccctttaga taagagttaa caaaataacc
      421 ccaggaagaa ggtaccggtt tccacgttac agacgttccc ggcataaagt gtccgaccag
      481 ggccttattc gcagttacca gcgccctgta tgccctgata ttttctggaa catggatgac
      541 cggggcagta atcataccgg gtttgactga ctcccctgat attctgacat aaaagggaat
      601 aaataaagcg acatgtggcc acattgtaaa caaacgttaa acaaaacaga atttttcagt
      661 tgactactac gagtaaataa aaaaatattt atcaaaaaca aacgagtgga atggagttaa
      721 cttgtctatg tacttaaaga ttcttgctac tgcactctca gctcctgtag cgtttgctgc
      781 actggcttct gatactgggc tttcttttac accggagaaa atcagtacgg agattgattt
      841 tggcacttta agtggcaagg ccaaagaacg ggtttatctg cctgaagaaa aagggcgcaa
      901 agccagccag ctggactgga aatacagtaa tgcgccaatt gtcaaggggg catttaactg
      961 ggatctgtta cccagagtat ctgtcggtgc ctcagggtgg acaacattag ctggtcgtgg
     1021 aggcaatatg gtggacaggg actggctgga tacgagcaat cccggaacct ggactgatga
     1081 gagtaaacat ccgaataccc gtctaaattt tgctaatgaa ttcgacctga atattaaggg
     1141 atggctcctg aatcaaccag actatcagtt aggtcttatg gctggttacc aggaaaatcg
     1201 ttatagcttt acagcaaaag gaggctctta tatctatagt agtgagggag gcttccggga
     1261 tgaaacggga tcatttccag atggtgaaag agctataggc tacaaacaac actttaaaat
     1321 gccctatata gggttaaccg gtaattatcg gtatgatagc tttgagttcg gggggagttt
     1381 caaatatagc ggatgggtga aggcatcgga taatgatgag cattacaacc ctgagaaaag
     1441 gatcacctat cgtagcgatg taaataatca gaactattac tctgtctcac tacatgctgg
     1501 ttattatatt acacctgcag ctaaagttta tgtcgaagga acatggaacc gtattactaa
     1561 taagaaaggt gatacttctc tgtactccag aaatttgaat atatctgatc acaccaaaaa
     1621 tggtgcaggc attgaaagct ataactttat gaccacagct ggtctgaagt attattttta
     1681 acctgaagca tgcctgtttt taactttttc atgacacttt aaatatgata aacaaaaggc
     1741 cagcacttct gatggtggtc ttttgatttt ttataatatg tggagggtat atgtgctcaa
     1801 cgataaaagt gtttctgtta actcttgtat ttttaaagat gctgccctgc cactcttcat
     1861 gcataacgac acagtgttat cgacataaaa ataattgata agcgatgtta acaatatgaa
     1921 ctttattata attctattac tcgtcaataa taagaagacc atccaataaa aacacacgca
     1981 ataacctgaa ggattctgcc gtataaaaat aaacattgtt tttacattta atctctcttg
     2041 attttgttta atcattgttt atgtaagata atttattttt ataatgatca catgaaaaca
     2101 aaagaggtta gatatgaaat tattattact tacaggtgca acaggatttc ttggtggcgc
     2161 ggtcctggat aagctgctgg ataactgtaa taatataaat ttgctacttt tagtacgagc
     2221 acctactcca caagcgggac tggaaagaat taaagaaaat atgcgtaaat ttaatgtttg
     2281 tgaggaaagg ttgcatgcat taactaatga taacatcttg cctggggatc taaataatcc
     2341 ggaagccttt ctcatggatc ctcgtcttga tgaagtcact catgttataa actgtgcggc
     2401 tatagcttct tttggtaata atccttttat atggaatgtg aatgttacag gtacacttgc
     2461 ttttgcaaga agaatggcaa aagtggcagg actgaaacgc ttccttcatg ttggtactgc
     2521 tatgtcttgt acacctcata cggggtcgct agttaaggaa gagtctgctt catcagaaac
     2581 aggtgaacat ttagtggagt atacgcattc aaaagcaaca atagaatatc tgatgcgtaa
     2641 gcagtgtcct gatttacctt tgttggttgc ccgaccatca attattgttg gccacagtcg
     2701 tttagggtgc ttaccttcaa ccagtatttt ctgggtattc agaatggggt taatgttgca
     2761 aaaatttatg tgctctctgg atgataaaat agatgttatc cctgtagatt attgtgctga
     2821 tgcattgcta atgttgcttg aaagctcgtt aattaatggt gagattgttc atatatcagc
     2881 aggtaaagaa agtagtgtga cgttctctgc tattgacgaa gctgtagccc gtgctttgaa
     2941 ctgtgatcct gttggagaca gatatactaa agtcagttat gacatactgg caatgagccg
     3001 tcatgatttt aaaaatattt ttggtccctg taacgaacgc cttatgttaa aagccattcg
     3061 tttatatgga gcgttcagta tgctcaatgt ttgtttcagt aacgacaagc tactgagtat
     3121 cggaatgcct aaaccgccaa agtttactga ttatattaaa tactgtatag aaacgacaaa
     3181 acacctttca attcaacaac aaatggaagt tgattttaaa taatatttat cataatcaga
     3241 attattgttg gtgttgcact cataatatat aaaaacgccg aggatatgac tttgtcaatt
     3301 aaaaatataa agcgcattat tacggcctgg aaaccatcta cattcgaaac gtacaaaaaa
     3361 acttttgaaa aatacggtgg tagtgtaaac atgcatccgg atgttgtttc atactttatg
     3421 atacatcatg actggaagtt tgattttttt cactatgaaa aagatggtga tatcaaaggc
     3481 tcatattttc tgtgcaatgg aaaacaaatt gggataatgg caagaaggtc ttatcctctt
     3541 tcatctgatg aggtattaat tcctttttcc ccacatgcaa gatgtttttt tccggataaa
     3601 actaataaat tatcaattat aaataaacaa aatattatca atgcaacatg gaaaatagcc
     3661 agaaaaaaac aaaattgtat tataaaggaa tctttttcac ctaaatttga aaaaactcgc
     3721 cgaaatgaaa tccagcgatt tatcaggaat ggcggagaga ttaagtgtat aagccagtta
     3781 tccgataaag aaatatccag ctcttatata tcactttttc attcccggtt tggaggtacc
     3841 ctaccttgct acgaatatga taatctgtta atgtttattt ctcacttacg agaacttatg
     3901 tttggccatg tgctattctg ggataataaa ccgtgtgcta ttgatatagt actgaaatct
     3961 gaaagctcat gtaatgttta ttatgacgtt ccaaatggtg cagttctgaa tgatgagaat
     4021 tgcatgaaac taagtccggg aagtgtcctt atgtggctga atatagataa agcgcgaagg
     4081 tattgtcagg ataataataa aaagatgata tattctatcg gtgcattcag acctgaatgg
     4141 aaatataaac tactatggtc tgttccctgt aaggttggta aatgcttatg ttagaatatg
     4201 tataaaggta agtaactata taagagcgtc cagtctgcaa atac
//
LOCUS       DAZFZC010000216         4166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFZC010000216 DAZFZC010000000
VERSION     DAZFZC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.220"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            238..765
                     /gene="sodC"
                     /locus_tag="ACHKXP_005058"
     CDS             238..765
                     /gene="sodC"
                     /locus_tag="ACHKXP_005058"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7649844.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            complement(952..1533)
                     /locus_tag="ACHKXP_005059"
     CDS             complement(952..1533)
                     /locus_tag="ACHKXP_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7649845.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTIIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(1530..2273)
                     /locus_tag="ACHKXP_005060"
     CDS             complement(1530..2273)
                     /locus_tag="ACHKXP_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7649846.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            complement(2279..2977)
                     /locus_tag="ACHKXP_005061"
     CDS             complement(2279..2977)
                     /locus_tag="ACHKXP_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7649847.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(2977..3306)
                     /locus_tag="ACHKXP_005062"
     CDS             complement(2977..3306)
                     /locus_tag="ACHKXP_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649848.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
     gene            complement(3303..>4166)
                     /locus_tag="ACHKXP_005063"
     CDS             complement(3303..>4166)
                     /locus_tag="ACHKXP_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7649849.1"
                     /translation="QQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPAALAK
                     ATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAA
                     MLTGSEQSWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQA
                     AAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYV
                     GGAGSPAQMRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQM
                     RDGGVFSGGRR"
BASE COUNT          891 a         1367 c         1001 g          907 t
ORIGIN      
        1 atcggtccct caccaatcgc atcaatcaca ctcatcatct gcgtggattt gagattatcc
       61 ttcgcctctc gaggcgtgtg cgccctgccg ccacctttgc ccataatgtt cctctcaatt
      121 ggtattatta atcgcagtga taggatattg cacagctatt gcgcgatatc atcagaacgc
      181 tgtttgttac cctgtaacca gcaagctcag tctgttaacg gaattaatga gggttttatg
      241 aaatgtaaaa tcattgctgc cattgccatg ctgacagcag catcatgcgg atacgcagca
      301 gaacaggaag tcccaatgaa ccttgtcagt gctgacggaa aagaagtcag cattggaaaa
      361 ataaccattc aggagacccc ctacggtctg ctgttcacac cagcccttca ctctctgtct
      421 gaaggcattc atggttttca tgtgcacgaa aaaggaaatt gcgccccggc actgaaagac
      481 ggaaaaccgg tcgcagcatt atcggctggc ggtcactttg acccgaaaaa caccggcaaa
      541 catcttggcc cctggtctcc ggatggacac ctgggcgacc tccctgcgct gttcgtgacg
      601 catgacggaa aagcgaacta cccggtcctg gccccgagac tgaactcatt aaaagagatt
      661 aaagggcgtt ctctcatgct tcatgctggc ggtgataacc atcatgacca tccggagccc
      721 ctgggcggtg gtggtgcgag aatggcctgc ggcatcattc aataatcagt caggtaaggg
      781 gcgggcccct tacctttatt cctcaggacg ataaatcctt tctccctgaa aagaacggca
      841 catcctccct ctctgagtta atgtttttgt cgtgacataa gaataattcc ttacactcaa
      901 tcttcatttt ctgttcccgc agccttcggt cactgcggga ttttttcgct tttatgcctg
      961 ccgcccgata accaccacct tcccgtcacc gccttcatca cgggtactga tgtcctggga
     1021 gattcgccgt gagccaacca gcatttcacc gtaaggcacc ggcatcgggt tcccctgagc
     1081 aatcatgttg tccagtgagg aaaaatacgt gttctgtctg ccgttatccg ttgcgcggta
     1141 atccggtgtt tttgccttcg gggcaagcat ctgagccaca ccacccagta tcatgctggc
     1201 ccccagtgaa aacagcatcg tggtggcaga aaaaccaccg gctgccaggg ctgaacccca
     1261 taacgccatt gatgccccgg cagtgaagaa agagcccacg atggctgccg cccccagcac
     1321 aatctgcagt ccaccctttc cggccccggc cagtcgcggc acaatgtgga tgatcgttcc
     1381 ctcacccagt tgttcgtgaa gacgggcgta caccgcctcc ggtgccgtgt cctcaccgcg
     1441 aatacgtatc tggtaccagc cttcgttcat ctgacggcgg aatcccggca tctgcatcga
     1501 cagggcacgg atggcttccg ctgccgtgtt cacatacagg ctgaggcggc ggccaaatcg
     1561 ttgtaaatcc ccgtgaaggc agatacgtgc cagtggcggt gacgccaggc tgaatgcgtt
     1621 cgtcgttgcc atttttcgga atacctctcc cgtttactca gttgttcagg cagatggtga
     1681 agcagctcac cgttgccgca gtatatggcg gcatgattgg ccaccgatgc gccaaagcag
     1741 cacagcagga tatcgccagg ctgtgcggaa ggcagggaaa tcctgtaaaa accagtcgcc
     1801 tccatattgt ccaggtacag gttctgaccg ttgcgccacc agtcatcctc acgctcaaaa
     1861 tccggcatat caattcccgc cagatggtag gcatcccgga acagcgtgta acagtccgtc
     1921 accccgtgct caaagcgccg tcctgtcaga tgtggcacac agcggaattt atgaatttcc
     1981 ccccggcaga ccagccacca ggacagtgca ctttttatct gcagccgccg gtcggcctcg
     2041 ctcagccagg gcagaccacc gggatgactg tggaccagtg ccacaatctc cccctgcatc
     2101 tctgcccgca gccagtcttc cggtgcaata cgaaaatacg cctccggctc tgcagagata
     2161 ttcacacaag ggatataccg ctccccctcc ggcgttctca ccacgaagcc gcacgactcc
     2221 gcaggcacac accgccgggc atgcgccaga atcgctgatt ctgtctgtgt cattggattt
     2281 actgcgaaag tttgttaatg gaaaggaaac cgccaaaatt agccaccatg ccgcgcatct
     2341 cacacccgcg catgcacttg ctgcatctgt ccttacggat atcggtggtg ggtttatcga
     2401 actcatccgc caccgcagga ccgttatacc cgcattcatc tccccggtaa tcccacatac
     2461 aggtgttcgc cagcatgatg cgaccgggaa acagcgctcc gtccgtctcc gtcggtgttg
     2521 ccagcacaaa cgaggctgtc atggccgtca gctctgacat ctgctccacc acccagcggt
     2581 cgctcagctc ctgctccggg tccgcttccg gattgcccgc cacaaaattc accgcatcca
     2641 gaaaacgggc atacacccgg cggcggacca ccgtggcccc caccaggctc tgcaggtcct
     2701 ccgccattcc ggtgaccaga ccgaacagat tcgacaccgt cagcgacggg cgggcactgc
     2761 tgcccttccc gttcatctca aagccactgc cgtcaatcgg gtatgcctga tattgccgcc
     2821 cctgccaggt aaccgcctcc cctttttcat tcagctcatt gcagaaaaaa taccgctcac
     2881 caccctgtac cgtcaggtcg atttcccaga gtaccacccg cggtgactgc tctgatttaa
     2941 ccgactcgtt cagactttct tcgtgaatat cctgcatcag ttcaccacct gcttaaactc
     3001 cgcgctgaat tcaacccgca gcatgcgaac ccgtgatgac caggcggcac aggtcacctt
     3061 tatctgccgc caggcataag gcggtgtcca cagaaacgcc ttccagccac cgtgctctgc
     3121 caggaatgcc tccagatgtc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt
     3181 tttcaggtca gcattcagcc ccgccgccat acgctgcgaa tacccgtcac caaaacgcac
     3241 ttcacgcacc gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaatgt
     3301 tttcatcgcc tgcctccgga aaagacgccg ccatcacgca tctgcgcctg aatctcatcc
     3361 tgcgccccct tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg
     3421 ttggtgccgt cgttctgaat caccacgtga ttgttctgat taaaactaat gccttccgcc
     3481 cgccgcatct gcgccggact tccggcaccg cccacataac caccttccgc atacccgcgc
     3541 atcagacgat acaggttgcc gacgccaatc cggctggttg cctccttcgt gaagacaaac
     3601 tccccgcggt ggacaatacc ggcaggttcg tatttgccac cggttcccgt aaatcctccg
     3661 gtcgcgaaat ggaagttcgc cgccgcagcc tgaatggccg tccccgtgga ggcagacgcc
     3721 ccaccaccga aagcaccacc catggcgctg ccaatactcc cgacaatccc caccatggcc
     3781 tgcttcagaa aaatctctgt cagcatggag agcacagaac gggtgaaacc acgccagctc
     3841 tgttcgctgc cggtcagcat cgctgccata ttctgtgcaa taccgtcaaa ggtctgcgtg
     3901 gccacgcttt taacctgcga aaaactgtcc gtcgcacttt ctgcccactc gccccagccg
     3961 gacttcagac cggccatcca gcttccacga agctgctcct ccgcagacca ggtgttcttc
     4021 agtgcagatg tggccttcgc cagcgcagcc ggattatcac cgtacacgtc acgaaggcgc
     4081 tgctcttccg actcccgctg cgcctgacgg tcggtgagtc cgcgggcttt tgcgctgatt
     4141 gccgcctgct tcgcactctg ctgctg
//
LOCUS       DAZFZC010000217         4063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFZC010000217 DAZFZC010000000
VERSION     DAZFZC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.221"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..230)
                     /locus_tag="ACHKXP_005064"
     CDS             complement(<1..230)
                     /locus_tag="ACHKXP_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO7649850.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATAD"
     gene            complement(273..509)
                     /gene="ycgZ"
                     /locus_tag="ACHKXP_005065"
     CDS             complement(273..509)
                     /gene="ycgZ"
                     /locus_tag="ACHKXP_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO7649851.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLHTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            823..2034
                     /gene="bluF"
                     /locus_tag="ACHKXP_005066"
     CDS             823..2034
                     /gene="bluF"
                     /locus_tag="ACHKXP_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO7649852.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMLSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            2239..2970
                     /gene="bluR"
                     /locus_tag="ACHKXP_005067"
     CDS             2239..2970
                     /gene="bluR"
                     /locus_tag="ACHKXP_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO7649853.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3191..3595
                     /gene="ycgX"
                     /locus_tag="ACHKXP_005068"
     CDS             3191..3595
                     /gene="ycgX"
                     /locus_tag="ACHKXP_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO7649854.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFRKYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3648..3758
                     /locus_tag="ACHKXP_005069"
     CDS             3648..3758
                     /locus_tag="ACHKXP_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649855.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            3994..4038
                     /locus_tag="ACHKXP_005070"
     CDS             3994..4038
                     /locus_tag="ACHKXP_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649856.1"
                     /translation="MFSKLAQSSIKAMF"
BASE COUNT         1217 a          837 c          812 g         1197 t
ORIGIN      
        1 ttgtcagctg tcgctgttct tcggtttggc cctcctgaat cactattgct aaaatcacga
       61 attatgctgt caattaaatc gcgtcggaaa gcatcttcct cagccgattt cattacgagt
      121 aattgcttaa taagagtctg caatgaaaaa tttatcttca tgcgcagtat ctggagcacc
      181 gcctggccag taatttcata ttttatacgt tcattatcag atgtcttcat aacgacttca
      241 tagctaacct gttaaataat caggctgttg cattattcaa aaagcaaccc aattagtgca
      301 ttataatgtt tctgttcctc ttccccagtc gcgtgttcca gtcggcaaag aagtttggca
      361 caaagcgact ttcgactcag attacgtcca tctttcaaaa tctcagtcac aatttccccg
      421 agagtttcct gctgagtatg caagttagct tttgcaaagt aacgagtgat tgctcccgca
      481 gaatctaaag tcactgaatt ttgatgcatg ctacgcctct gttaaaaatg ttaatcaatg
      541 ttaagtgaag taacaatagc aaacttgtac gaaatatgtg tacagaatga taaattaatt
      601 ttttgcaatt agtgctaatg catataaaac aatgtgttat gataaaacat attgcatcat
      661 ttaactgaca atctatacaa gatttttcgc caactagtgt tcaacttttt ccatttttta
      721 tcaatgtaaa gaaacggtta tgttttggta ttaaaagaaa gacacaagaa gatatgtctg
      781 ttaccgtctt actctcgcct cacccattac cctggattgt taatgcttac cacccttatt
      841 tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa tcgaagaaat gctttcgata
      901 gcaaatcgca ggaacatgca gtctgacgta acagggatct tactgtttaa tggttctcat
      961 tttttccagc ttctggaagg tccggaagaa caggttaaaa tgatatatcg ggctatatgc
     1021 caggatccac ggcactataa tattgttgag ctgctgtgcg attacgcgcc tgctcgccgt
     1081 tttggcaaag cgggaatgga attatttgat ttgcgcctgc acgagcgaga tgacgtttta
     1141 caggccgtat tcgacaaagg cacatcaaaa tttcagctaa cttatgatga cagagcgcta
     1201 caattttttc gtacttttgt ccttgcaacc gaacaatcaa cctatttcga gatccctgcc
     1261 gaagactcct ggctttttat cgctgacgga tctgataaag aacttgattc ctgtgccctt
     1321 tcaccaacta taaacgacca ctttgccttt catcctattg tcgatccctt atcgcggcgg
     1381 ataatcgctt ttgaagccat tgtgcaaaaa aatgaagata gcccatcagc catagcggtt
     1441 gggcagcgta aagacgggga aatctacaca gcggatctca aaagtaaggc gcttgcattc
     1501 acgatggcac acgcacttga gctcggtgat aaaatgattt caatcaatct attacctatg
     1561 accctggtta acgaacctga cgcagtctct tttttactta atgaaataaa ggccaatgct
     1621 ctggtgcctg aacaaatcat cgttgaattt actgaaagtg aagtcatatc tcggtttgat
     1681 gagtttgccg aagcgattaa atcgctaaag gctgctggta tcagtgtagc aattgatcat
     1741 tttggcgcag gttttgctgg tttgttactc ctgtcacgct tccagcctga cagaattaaa
     1801 atcagtcagg aattgattac caatgttcat aaaagtgggc cacggcaggc aattattcag
     1861 gcgatcataa aatgctgtac atcacttgaa attcaagtca gtgctatggg cgtggcaaca
     1921 ccagaagagt ggatgtggct tgaatccgca ggaattgaga tgtttcaggg agatctgttt
     1981 gctaaagcta aattgaatgg tatcccttcc atagcgtggc cagagaaaaa ataattttca
     2041 gcacattctt tcacatgatt tcagtaaatc atcgtgaaaa tgaatacgtt acgtttaaat
     2101 ttaatccaca ttaaaaataa tgtttcatcc aatttagctg aaatcttatt tatactttgt
     2161 acaattttag cgtatattgt acaaagtatt attgggtcgt gtacaggcga cggagatttg
     2221 tgaccgcaag gaggaattgt ggcttattac agcattggtg atgttgctga acgttgcggg
     2281 attaatcctg tcactctccg ggcctggcaa cgccgctacg gtttgttaaa accacagcgc
     2341 agtgaaggcg gacaccgact ctttgatgaa gaagacatac aacgcatcga agagatcaag
     2401 cgttggataa gtaatggcgt ccctgtaggc aaagttaaag cattactgga aaccaccagc
     2461 caggatacgg aagatgactg gagccgcctg caagaagaga tgatgtcaat tcttcgcatg
     2521 gctaatcctg ccaaactacg cgcgagaatt atttcactgg gtcgagagta cccagtagat
     2581 caattgatta atcatgttta ccttcctgtt cgccagcgtc tcgtgcttga tcacaacacc
     2641 tcccgcatta tgagcagtat gtttgacggc gcattaattg aatacgcagc aacctcgctt
     2701 ttcgaaatgc gccgtaagcc cggtaaagaa gccattctga tggcgtggaa tgttgaagag
     2761 agagcacgat tgtggctgga agcatggcgt ttatcattgt caggatggca catttctgtt
     2821 cttgctgatc ccattgaatc gccgcgcccg gaactgttcc cgacgcaaac attgattgtc
     2881 tggacaggca tggcaccaac gagaaggcaa aacgaacttt tacaacactg gggtgagcaa
     2941 ggttataaag tcatctttca tgccccctaa tttcaccttt atcccgcccc ttagacagaa
     3001 tgttataatc tgaacccaca acagcgtttg taccggagct ggtccgttgc aaaatgctca
     3061 ccgggagaat tcagatattc cctctatcac actctgtaca tatcacagcc aaaggacaga
     3121 ccagattgaa gtggtaaaac cgatcgtgca cactacgcag tctcacaact aacaagaggt
     3181 tattatcctg atggatcagg tcgttgtttt tcaaaagatg tttgagcaag tgagaaaaga
     3241 gcaaaacttc tcctggtttt attcagaatt aaaacatcac cgtattgcac attacattta
     3301 ttatctggct acggataaca tcagaattat tactcacgat gatacggttt tgttattaag
     3361 aggaaccagg aacctgttaa aagttagtac gaccaagaac cctgctaaaa taaaagaggc
     3421 cgcattgctt catatttgcg gaaaatctac atttcggaaa tactgttcaa cactggcagg
     3481 cgcgggcgtt ttccggtggg ttactgatgt taatcataac aaacgcagtt actacgccat
     3541 tgataatacg cttttatata ttgaagatgt agaaaataat aaaccattaa tctagctaaa
     3601 gttggatgct taagaaatgc ttcataattc agtaaggcat tagcataatg ggaataaaag
     3661 tacagagact atccctatgg atgataaata ctgtcctttt attgctaccc ataaataatc
     3721 accatgccaa tactatcaaa ttgatatttg aaatgtgatc acttgacttt tgatacgtta
     3781 ttttataacg gttaacatat ttataaaaac aacggccgtg ccacacgtcc gtttcaatac
     3841 ttaacgcaca tgtattttgg tttagtcatc atccggttat atgtatttta gccaggaaca
     3901 ggttaaacct ttcctatata actcaaaaat tgaaacctta ttctcatgtc atgcttatat
     3961 tcattattat cgttatataa aaaggcaacc ataatgttta gcaaattggc acaaagtagc
     4021 ataaaggcta tgttttaatt acaggatgtt cagtcatttg aat
//
LOCUS       DAZFZC010000218         4056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFZC010000218 DAZFZC010000000
VERSION     DAZFZC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.222"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..445
                     /gene="cesT"
                     /locus_tag="ACHKXP_005071"
     CDS             <1..445
                     /gene="cesT"
                     /locus_tag="ACHKXP_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO7649857.1"
                     /translation="DRFAEKIGIGSISFNENRLCSFAIDEIYYISLSDANDEYMMIYG
                     VCGKFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLDDATPEKLEN
                     EIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            506..3352
                     /gene="eae"
                     /locus_tag="ACHKXP_005072"
     CDS             506..3352
                     /gene="eae"
                     /locus_tag="ACHKXP_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type epsilon"
                     /protein_id="HFO7649858.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVTGHTNKMTKMSPDVTKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADNVDTITYTATVKKNGVAQANAPVTFSIVSGTATLGANSAKTDGNGKATVTL
                     KSGTPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKANGSDAITYTV
                     KVMKNNQPEANHSVTFSTNFGNLGGNSNTQIVKTDKDGRATVKLTSGVAGNAIVSAKV
                     SEVNTEVKAPEVKFFSVLSIDSNVNIIGTSATGALPNIWLQYGQFKLTAKGGDGKYQW
                     RSQDPKVASVDALTGRVTLLKKGTTTIEVVSGDNQTATYTINTPIKIISVETKNKVVY
                     NDAEAICRTNNGRLPLSTNELKDVYNKWGAANSYEGYKGKNTITAWTQQTEDDKTKGW
                     TSTFDIVTKNEIPSNGSNNKVNVTAANAFAVCVR"
     gene            complement(3731..>4056)
                     /gene="escD"
                     /locus_tag="ACHKXP_005073"
     CDS             complement(3731..>4056)
                     /gene="escD"
                     /locus_tag="ACHKXP_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO7649859.1"
                     /translation="NDVIIVAGEVSQQNESKILAIINAMNKNSNVKILFQNIQPYISA
                     DIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGINISRPDEYIH
                     IPLSY"
BASE COUNT         1228 a          754 c          887 g         1187 t
ORIGIN      
        1 agatagattt gcagaaaaaa ttggtattgg atctatttca tttaatgaaa atagattgtg
       61 ttcttttgct attgatgaga tttattatat ttcgttatct gatgctaatg acgaatatat
      121 gatgatctat ggcgtctgtg ggaaattccc gacagataat cctaacttcg ctcttgagat
      181 tttgaatgca aacttatggt ttgcagagaa tggtggacca tacctgtgtt atgaatcagg
      241 agcacaatcg ctgttgttag cgttacgttt tccgctcgat gatgctacac ctgaaaaact
      301 tgagaatgaa atagaagtcg tcgttaagtc aatggaaaac ctgtatttgg tattacataa
      361 tcagggaata acattagaaa acgaacatat gaaaatagag gaaattagtt caagcgataa
      421 taaacattat tacgccggaa gataaaagat tatttattaa tataatttac tactcattct
      481 aactcattgt ggtggagccc ataacatgat tactcatggt ttttatgccc ggacccggca
      541 caagcataag ctaaaaaaaa catttattat gcttagtgct ggtttaggat tgttttttta
      601 tgttaaccag aattcatttg caaacggcga aaattatttt aaattgagtt cagattcaaa
      661 actgttaact caaaatgccg ctcaggatcg ccttttttat acgttaaaaa caggtgaaac
      721 tgttgccaat atttctaaat cacagggtat cagtttatcg gtaatttggt cactgaataa
      781 acatttatac agttccgaaa gcgaaatgat gaaggctgga cctggtcagc agatcatttt
      841 gccactcaaa aaactgtctg ttgaatatag tgccttacct gtcttaggtt cggcacctgt
      901 tgttgctgca ggtggtgtca ctggtcatac gaataaaatg actaaaatgt ccccggacgt
      961 gactaaaagc aacacgaccg atgacaaggc tctaaattat gcggcacaac aggccgcgag
     1021 ccttggtagc cagcttcagt cgcgctcact gaacggcgat tacgcgaaag ataccgctct
     1081 tggtatggcc agcagccagg cttcatcaca gttgcaggcc tggttacaac attatggaac
     1141 ggcagaggtt aatctgcaga gcggtaataa ctttgacggt agttcactgg acttcttatt
     1201 accgttctat gattccgaaa atatgctggc atttggtcag gtcggggcgc gttacattga
     1261 ctcccgcttt acggcaaatt taggtgctgg ccagcgtttt ttccttcctg aaaatatgtt
     1321 gggctataac gtcttcattg atcaggattt ttctggtgat aatacccgtt taggtattgg
     1381 gggcgaatac tggcgagact atttcaaaag tagcgttaac ggctatttcc gcatgagcgg
     1441 ctggcatgag tcatacaata agaaagacta tgatgagcgc ccggcaaatg gttttgatat
     1501 ccgctttaat ggctatttac catcatatcc ggcattaggc gccaaactga tgtacgaaca
     1561 gtattatggt gataatgttg ctttgtttaa ttccgataag ttgcagtcga atcctggcgc
     1621 ggcgaccgtt ggtgtaaact acactccgat tcctctggtg acgatgggga tcgattaccg
     1681 tcatggtacg ggtaatgaaa atgatctcct ttactcaatg cagttccgtt atcagtttga
     1741 taaaccgtgg tctcagcaaa tcgagccaca gtatgttaac gagttaagaa cattatcggg
     1801 cagccgttac gatctggttc agcgtaataa caatattatt ctggagtaca aaaagcagga
     1861 tattctttct ctgaatattc cgcatgatat taatggtact gaacacagta cgcagaagat
     1921 tcaattgatc gttaagagca aatatggtct ggatcgtatc gtctgggatg atagtgcatt
     1981 acgcagtcag ggcggtcaga ttcagcatag cggaagccaa agcgcacaag actaccaggc
     2041 tattttgcct gcttatgtgc aaggtggcag caatatttat aaagtgaccg ctcgcgccta
     2101 tgaccgaaat ggtaatagtt ctaataatgt acagctcact attaccgttt taccgaatgg
     2161 gcaggttgtg gaccaggttg gggtaacgga ctttacggct gataagacat cggctaaagc
     2221 ggataacgtt gataccatta cttataccgc gacggttaaa aagaatggtg tagctcaggc
     2281 taatgcccct gtaacattta gtattgtatc cgggactgca actctcggag caaatagtgc
     2341 caaaacggat ggtaacggta aggcaaccgt aacgttgaag tcgggtacgc cagggcaggt
     2401 cgtcgtgtct gctaaaaccg cggagatgac ttcgccactt aatgccagtg cggttatatt
     2461 tgttgatcaa accaaggcca gcattactga gattaaggct gataaaacaa cagcgaaggc
     2521 aaatggttct gatgcgatta cctatactgt taaagtaatg aagaataacc aaccagaagc
     2581 aaaccattct gttacattct caacgaactt tggtaatctg ggggggaatt ctaataccca
     2641 aattgtgaaa acggataaag atggtagggc tacggtaaaa ctgacatctg gcgttgcagg
     2701 taatgctatt gttagtgcaa aagtcagcga agttaataca gaggttaagg ctcctgaggt
     2761 aaaattcttc tcagttctga gcattgatag taatgttaat attattggaa cctctgctac
     2821 tggcgctttg cctaatattt ggttgcaata tggtcagttc aaattgactg ctaaaggtgg
     2881 tgatgggaaa tatcaatggc gctctcaaga tccaaaagtt gcatcagttg atgctttaac
     2941 tggtcgagtt actttgttga agaaaggaac aacaacaatt gaagttgtgt cgggtgataa
     3001 ccaaactgca acgtatacaa ttaatacacc tataaaaatt atatctgtgg agacaaaaaa
     3061 taaagtagtc tataacgatg ctgaagcaat atgtagaacg aataatggcc gtttaccgct
     3121 atctacgaat gagttaaagg acgtgtataa taaatgggga gcggccaata gttatgaagg
     3181 ctataaaggt aaaaacacaa taacagcatg gactcagcaa acagaggatg ataaaactaa
     3241 aggttggact agtacatttg acatagttac taaaaatgaa atccctagta atggaagtaa
     3301 taataaggtc aatgtgacag cagctaatgc ctttgctgtc tgtgtaagat gatcttataa
     3361 ccattaatta atgcccaact aagatagtta tcttggttgg gcttaataga catttattca
     3421 accttaatag ttgtacctta tttaggtaaa tttctttacg gcgagaccat tattgaattg
     3481 ctcaactgcg cttgtttaat attctgtata aatgcttctc tcagaactgc ccattcttgt
     3541 gataaccgag ctcgataatt aaatggctca ttatcatacg tctgatcccc tggcgacatt
     3601 ctctgttact atgatagcga caggattgct attcacatta cttttccacc gatccccatt
     3661 agaaaatttg ctattacgtc accctacaat aaataaaaaa ccctccgaag agggggaaag
     3721 tgataataag ttaataagac agcggaatat gtatatattc gtctgggcgg gagatgttga
     3781 taccgtcttt aggatcaatc gcgtctatga catagcctcc tttcagaatg ctaccaattc
     3841 ccaggcttgt accattatca agagcaattg ttggattctt catcgtgccg ctgattctga
     3901 gtatttttcc ggggaaaata tctgctgaaa tgtacggctg aatgttctga aatagtatct
     3961 tgacgttact atttttgttc atcgcattta taatggcaag aatcttactc tcgttctgtt
     4021 gagaaacctc ccccgcaaca atgatgacat cattct
//
LOCUS       DAZFZC010000219         3983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFZC010000219 DAZFZC010000000
VERSION     DAZFZC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.223"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(167..1015)
                     /locus_tag="ACHKXP_005074"
     CDS             complement(167..1015)
                     /locus_tag="ACHKXP_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO7649860.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKERLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQLLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDASGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(1061..1339)
                     /gene="yacB"
                     /locus_tag="ACHKXP_005075"
     CDS             complement(1061..1339)
                     /gene="yacB"
                     /locus_tag="ACHKXP_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFO7649861.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(1339..1608)
                     /gene="yacA"
                     /locus_tag="ACHKXP_005076"
     CDS             complement(1339..1608)
                     /gene="yacA"
                     /locus_tag="ACHKXP_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO7649862.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(2252..>3983)
                     /locus_tag="ACHKXP_005077"
     CDS             complement(2252..>3983)
                     /locus_tag="ACHKXP_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HFO7649863.1"
                     /translation="AAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKF
                     YEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQ
                     YLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQL
                     LPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESS
                     PGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGG
                     RGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLR
                     IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTL
                     NIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFI
                     LDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTA
                     AIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGK
                     FRPLRMPGKP"
BASE COUNT          772 a         1148 c         1167 g          896 t
ORIGIN      
        1 ctgaggaaaa caaatgaatt aactgcttac gtatgtggat acatatacag tattaattta
       61 ttaaggagaa aatggcatgt caagatttaa tgatagtgat tatcattaac atgaagtgat
      121 tttacgtctg gataacgtaa atgattgcgg tatacaggcc aggcggtcag attttcagcc
      181 gtgcctgttc ctgcagcagt tccagatgat atgcagcaat agcgtttacc acccctgccg
      241 tcatccgtgc gtcggcgatc gctgagtgtg cctgcccttc ccaggtcagt ccggcctggc
      301 tggcagcaca ggccagtgaa atcgtgccat agcggttggt ggccccataa tatcctgcag
      361 ccagctccat cgcacaatat accgtcagtt cttccagcca gtcagccgga tcgctatgtg
      421 cggcagcagt ctgtttcaga atgcggatgt cgaaccgggc attaaagata attactggtc
      481 ggtccccgat tgcatgacgc agttgccgcg ccacatctgt ccatgatggt gcgccgcaga
      541 gagcctgctc gctgatacca tgcacagcgg cggcctgcgc cccgatggcg accgtgggct
      601 tcagcctggt ttcaaatacc acctgaccgg acgcatcggt caggccgatt tccagagctt
      661 ccgcggtatt gtccagtcct gtcgtttcag tatccagaaa gagcggtgcc agactcagcc
      721 agtcatatgc ctgccgtgcc atccggccac tggtgctgtt aagtctggat aaaacggaaa
      781 gtcgctggcc agccagaagt tgtttactcg tcagtgggcg tttctcacgc atcggaacac
      841 agtctgcaat tcggtataca gcgaacctgc caccataagt gtttttatac cacttaaccg
      901 gctcagcacc gggggctggc ttcattctga attcatcgcg cagccgttct ttcgagaaac
      961 acctgtcttc ggcaatcgcc tgcgcacgtc tggtggccag ctcatcatta ttcatggttc
     1021 ttgcatctcc gtcagacctt tttatatccg gacagcccct ctatggccac ttctgtgcag
     1081 tatgcaatac acgcaggata tacacttttc cccactggct gtcgacctca taaaccagaa
     1141 caaaatgcgg gtgaataacc agttcccggg taccttccac acgaccgttg cggcctttat
     1201 acggctgccc ggccaggctg ctggctgaat aaccaatccg ttcgtccagt tcaatggcgg
     1261 ccatcaggtt ctgctccgca atgtactcac gaatgcgctt cctgtcctgg ctggccagca
     1321 tggtccagaa tatctccatc atttaccggc gactttcctg ctcatttcat cccgccatgc
     1381 agcagcggaa gattcgacca tgtcatgggg aagcacatcg ccgcgctcga gttgctgacg
     1441 tcctgcctca acctggtcac ggaaccaggt attatgctgc cgttccacgg tctggcgcat
     1501 aaaatcccgg attaactgag agccattacg gtccatgctt tttgcagcgg ccataaaagc
     1561 atccttcagt tcagcgtcaa ttcttaaact catattaacc tgtgccattt atcacctcgt
     1621 cattactacg tatatacaca gtatataacg attcagatat cgtcaccaga atatgccgcg
     1681 tcagcggcat ggaaggcggc actctgttgt tgcatatgac acaggagtaa aaccgccgaa
     1741 gcccggcgta agccggtacg gactgataga tttcacctta cccatcccca gccctgctag
     1801 gccatatccg ctttcagcca tgataaagct tctgtgcgcg gtcggagtgg tcccgacgag
     1861 ggtttacccg aagtcggggc gtatctccgc gttagcgggc cgtcagggcc gcttacgagc
     1921 gtgtactcaa tactttcagc gagaagactg acagccatga tggagtatag ttacaacatt
     1981 cataattaaa agcgactctg ttccggcccg aagggccggg gcggggccgc ttttcagtta
     2041 tgagggaggg gctttgtggt ttcagttctg cgctggttcg gggtttttct ggaggttggt
     2101 tttgtgtgtt gtaactaaag tggctccggt tggggcccgc cgtttacggt gggaggtgca
     2161 tatctgtctg tccacaggac aggcagtgaa taggttttct ttttaaatga atgtaattgg
     2221 ggagcccgca gaattcggaa aaaatcgtac gctaaggttt tccgggcatc cgtaagggcc
     2281 gaaacttccc gtcttccagt ctgcggctct gccgccagac gtaatcgccg gttaggttga
     2341 tgtgctccca gcccagcggc gacaggaatt gcagcagctc gccgtccacc ggcttgccgg
     2401 cctcgaccaa cccctgggtg gcgcgttcca ggtacaccgt gttccacagc acgatagccg
     2461 ccgtcaccag gttgaggccg ctggcccggt agcgctgctg ctcgaagctc cgatccctga
     2521 tttccccaag gcggttgaag aacaccgccc tggccagcga gttgcgcgcc tcacctttgt
     2581 tcaggccggc atgcacgcgg cggcgcagtt caacactttg cagccagtcc aggatgaaca
     2641 gcgtgcgctc gatccggccc agctcgcgca gggccacggc cagtccgttc tggcgcgggt
     2701 agctgccgag cttgcgcagc atcagcgagg cggtgacggt gccctgcttg atcgagctgg
     2761 ccaggcgcag gatgtcgtcc cagtgggcac gcacgtgctt gatgttcagg gtgccgccga
     2821 tcagcgggcg caacgtcggg taggcttgca cgccctgcgg cacgtacagc ttggtttcgc
     2881 cgaggtcgcg gatgcgcggc gcgaagcgga agcctagcag gtgcatcagg gcaaagacgt
     2941 gatcggtgaa gccggccgtg tcggtgtagt gctcctcgat ccgcaggtcg gactcgtggt
     3001 acagcaggcc gtcgagcaca taggtggaat cgcggacgcc gacattcacc acgcgggtgc
     3061 tgaacggcgc gtactggtcg gagatatggg tatagaacag ccgtcccggc tcgctaccgt
     3121 acttcgggtt gacgtgcccg gtgctctcgc cccggccacc cgcgcggaag cgctggccat
     3181 cggaggatga ggtcgtgccg tcgccccagt gggcggcaaa ggcgtggcga tactggtggt
     3241 tgaccagctc ggccaaggcc gccgaatagg tttcgtcgcg gatgtgccag gcttgcagcc
     3301 aggacagctt ggcgtaggtc aggccggggc tcgactcggc catcttggtc agcccgaggt
     3361 tgatcgcatc accgaggatt gcggacagca gcaacgtcct gtctttggcc tcggccccgt
     3421 ccttcaagtg ggtgaagtgg cggctgaagc ccgtccagtc gtccacgtcc atcagcagtt
     3481 cggtgatctt gatgcgcggc agtaactggc tggtttggtc gatcagcgcc tgcgcccgat
     3541 ccggcaccgc cgcatccagc ggggtgattt tcagccctga ctcggtgagg atggcatcgg
     3601 gcagctcgtt gtccttggcc aggcgggtga cggtggccaa ctgctcgtcc agcagctgca
     3661 aacgctcttc caggtactgg tcgctgttcg ggttgatcgc caggggcagg gcctgctcgc
     3721 gcttgagtgc ggcgaacttc tcggccggca gcaggtagtc gtcgaagtcg cggaactgcc
     3781 gcgagccctt gacccagatg tcgccggagc gcagggcgtt cttcagctcg gacagggcgc
     3841 agatttcgta gaatttccgg tcgaggcctt ccggggtgat caccagcggc ttccagcgcg
     3901 gcttgatgaa ggccgtgggt gcatcggccg gcaccttgcg caggttgtcg gcgttcatct
     3961 cacgcagggt ctgcacggct gcc
//
LOCUS       DAZFZC010000220         3888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFZC010000220 DAZFZC010000000
VERSION     DAZFZC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.224"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..337
                     /locus_tag="ACHKXP_005078"
     CDS             <1..337
                     /locus_tag="ACHKXP_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649864.1"
                     /translation="VIKLGNARIVLSRRRRRKKGQRSALKGGGSVLVVGNRRIPGAFI
                     QQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTTAFKQNIERIRRERLPKELGYALQ
                     HQLRMVIKR"
     gene            334..729
                     /locus_tag="ACHKXP_005079"
     CDS             334..729
                     /locus_tag="ACHKXP_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7649865.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            737..1477
                     /locus_tag="ACHKXP_005080"
     CDS             737..1477
                     /locus_tag="ACHKXP_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649866.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASSSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            1493..1915
                     /locus_tag="ACHKXP_005081"
     CDS             1493..1915
                     /locus_tag="ACHKXP_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7649867.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEVAGPAEPVSAGKCSTVS"
     gene            1897..2331
                     /locus_tag="ACHKXP_005082"
     CDS             1897..2331
                     /locus_tag="ACHKXP_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7649868.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2324..>3888
                     /locus_tag="ACHKXP_005083"
     CDS             2324..>3888
                     /locus_tag="ACHKXP_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7649869.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLALSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLS"
BASE COUNT          895 a          966 c         1252 g          775 t
ORIGIN      
        1 cgtaataaag ctgggtaacg cgcggattgt cctgtcccga cgcaggcgtc gtaaaaaggg
       61 gcagcgttca gccctgaaag gtggcggcag cgtgcttgtg gtgggaaacc gtcgtattcc
      121 cggcgcgttt attcagcaac tgaaaaatgg ccgctggcat gtcatgcagc gtgtggccgg
      181 gaaaaaccgt taccccattg atgtggtgaa aatcccgatg gcggtgccgc tgaccacggc
      241 gtttaaacag aatattgagc ggatacggcg tgagcgtctt ccgaaagagc tgggctatgc
      301 gctgcagcat caactgagga tggtaataaa gcgatgaaac atactgaact ccgtgcagcc
      361 gtactggatg cactggagaa gcatgacacc ggggcgacgt tttttgatgg tcgccccgct
      421 gtttttgatg aggcggattt tccggcagtt gccgtttatc tcaccggcgc tgaatacacg
      481 ggcgaagagc tggacagcga tacctggcag gcggaactgc atatcgaagt tttcctgcct
      541 gctcaggtgc cggattcaga gctggatgcg tggatggagt cccggattta tccggtgatg
      601 agcgatatcc cggcactgtc agatttgatc accagtatgg tggccagtgg ctatgactac
      661 cggcgcgacg atgatgcggg cctgtggagt tctgccgatc tgacttatgt cattacctat
      721 gaaatgtgag gacgatatgc ctgtaccaaa tccagcaata ccggtgaaag gtgccggaac
      781 caccctgtgg gtttataacg ggagcggcga cccttatgcg aacccgcttt cagacgttga
      841 ctggtcgcgt ctggctaaag ttaaagacct gacgcccggc gaactgaccg ctgagtccta
      901 tgacgacagt tatctcgatg atgaagatgc ggactggacc gcgaccgggc aggggcagaa
      961 atccgccgga gataccagct tcacgctggc gtggatgccc ggagagcagg ggcagcaggc
     1021 gctgctggcg tggtttaatg aaggtgatac ccgtgcctat aaaatccgct tcccgaacgg
     1081 cacggtcgat gtgttccgtg gctgggtcag cagtatcggt aaggcggtga cggcgaagga
     1141 agtgatcacc cgtacggtga aggtcaccaa tgtgggccgt ccgtcaatgg cagaagatcg
     1201 cagtacggtg acggcggcaa ccggtatgac tgtgacgcct gccagctcct cggtggtgaa
     1261 agggcagagc accacgctga ccgtagcatt ccagccggag ggcgcaactg acaagagctt
     1321 ccgtgcggtg tcagcggata aaacaaaagc caccgtgtcg gtcagtggta tgaccatcac
     1381 cgtgaaaggc gttgctgcag gtaaggtcaa cattccggtt gtatccggta atggtgaact
     1441 tgctgcggtt gcagaaatca ctgtcaccga cagttaatcc ggagagtcag cgatgttcct
     1501 gaaaaccgaa tcatttgaac ataacggcgt gaccgtcacg ctttctgaac tgtcagccct
     1561 gcagcgtatt gagcatctcg ccctgatgaa acggcaggca gaacaggcgg agtcagacag
     1621 caaccggaag tttactgtgg aagacgccat cagaaccggc gcgtttctgg tggcgatgtc
     1681 cctgtggcat aaccatccgc agaagacgca gatgccgtcc atgaatgaag ccgttaaaca
     1741 gattgagcag gaagtgctta ccacctggcc cacggaggca atttctcatg ctgaaaacgt
     1801 ggtgtaccgg ctgtctggta tgtatgagtt tgttgtgaat aatgcccctg aacagacaga
     1861 ggtcgccggg cccgcagagc ctgtttctgc gggaaagtgt tcgacggtga gctgagtttt
     1921 gccctgaaac tggcgcgtga gatggggcga cccgactggc gtgccatgct tgccgggatg
     1981 tcatccacgg agtatgccga ctggcaccgc ttttacagta cccattattt tcatgatgtt
     2041 ctgctggata tgcacttttc cgggctgacg tacaccgtgc tcagcctgtt tttcagcgat
     2101 ccggatatgc atccgctgga tttcagtctg ctgaaccggc gcgaggctga cgaagagcct
     2161 gaagatgatg tgctgatgca gaaagcggca gggcttgccg gaggtgtccg ctttggcccg
     2221 gacgggaatg aagttatccc tacttccccg gatgtggcgg acatgacgga ggatgacgta
     2281 atgctgatga cagtatcaga agggatcgca ggaggagtcc ggtatggctg aaccggtagg
     2341 cgatctggtc gttgatttga gtctggatgc ggccagattt gacgagcaga tggccagagt
     2401 caggcgtcat ttttccggta cggaaagtga tgcgaaaaaa acagcggcag tcgttgaaca
     2461 gtcgctgagc cgacaggcgc tggctgcaca gaaagcgggg atttccgtcg ggcagtataa
     2521 agccgccatg cgtatgctgc ctgcacagtt caccgacgtg gccacgcagc ttgcaggcgg
     2581 gcaaagtccg tggctgatcc tgctgcaaca gggtggtcag gttaaggact ccttcggcgg
     2641 gatgatcccc atgttcaggg ggcttgccgg tgcgatcacc ctgccgatgg tgggggccac
     2701 ctcgctggcg gtggcgaccg gtgcgctggc gtatgcctgg tatcagggca actcaacctt
     2761 gtccgatttc aacaaaacgc tggccctttc cggcaatcag tcggggctga cggcagatcg
     2821 tatgctggtc ctgtccagag ccgggcaggc ggcagggctg acgtttaacc agaccagcga
     2881 gtcactcagc gcactggtta aggcgggggt aagcggtgag gctcagattg cgtccatcag
     2941 ccagagtgtg gcgcgtttct cctctgcatc cggcgtggag gtggacaagg tcgctgaagc
     3001 cttcgggaag ctgaccacag acccgacgtc ggggctgacg gcgatggcac gccagttcca
     3061 taacgtgacg gcggagcaga ttgcgtatgt tgctcagttg cagcgttccg gcgatgaagc
     3121 cggggcattg caggcggcga acgaggccgc aacgaaaggg tttgatgacc agacccgccg
     3181 cctgaaagag aacatgggca cgctggagac ctgggcagac aggactgcgc gggccttcaa
     3241 atccatgtgg gatgcggtgc tggatattgg tcgtcctgat accgcgcagg agatgctgat
     3301 taaggcagag gctgcgttta agaaagcaga cgacatctgg aatctgcgca aggatgatta
     3361 ttttgttaac gatgaagcgc gggcgcgtta ctgggatgat cgtgaaaagg cccgtcttgc
     3421 gcttgaagcc gcccgaaaga aggctgagca gcagactcaa caggacaaaa atgcgcagca
     3481 gcagagcgat accgaagcgt cacggctgaa atataccgaa gaggcgcaga aggcttacga
     3541 acggctgcag acgccgctgg agaaatatac cgcccgtcag gaagaactga acaaggcact
     3601 gaaagacggg aaaatcctgc aggcggatta caacacgctg atggcggcgg cgaaaaagga
     3661 ttatgaagcg acgctgaaaa agccgaaaca gtccggcgtg aaggtgtctg cgggcgatcg
     3721 tcaggaagac agtgctcatg ctgccctgct gacgcttcag gcagaactcc ggacgctgga
     3781 gaagcatgcc ggagcaaatg agaaaatcag ccagcagcgc cgggatttgt ggaaggcaga
     3841 aagtcagttc gcggtactgg aggaggcggc acaacgtcgc cagctatc
//
LOCUS       DAZFZC010000221         3875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFZC010000221 DAZFZC010000000
VERSION     DAZFZC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.225"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            146..520
                     /locus_tag="ACHKXP_005084"
                     /pseudo
     CDS             146..520
                     /locus_tag="ACHKXP_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515619.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(662..1243)
                     /locus_tag="ACHKXP_005085"
     CDS             complement(662..1243)
                     /locus_tag="ACHKXP_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFO7649870.1"
                     /translation="MDQHFQTKDTPESRRVLEGQIRECYGRVVYSHKTHEKCSDILLS
                     RLSSIKFWQIILSALTTGGFLATFFGAGEVGTGVGIVVSTLLLILNAYTKDYDLGELA
                     QKHKQAANEIWLIREKYLSLLTDLAMGEKPIEQLQSERDSLLESLHSVYSGSPSTTFE
                     AYKKAQDALKNKEDLTFSEEEIDAFLPKELKRR"
     gene            complement(1221..2102)
                     /locus_tag="ACHKXP_005086"
     CDS             complement(1221..2102)
                     /locus_tag="ACHKXP_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFO7649871.1"
                     /translation="MGLAEWFCGFCSNLTVKDGGTISTRYRNITKRLNTDFWDSSSDT
                     SHSLYVGSYGRNTATQGLSDLDMIFQLPYSEYQKYNSYLGNGQSALLQAVKRSIEKTY
                     ATTSIRGDGQVILVPFNDGITFEVVPAFLNVSDSYTFPDANGGGRWRITNPKPEISAM
                     RLRNNITNNNLVQLCRMARAWKRKWDVPISGLLIDTLAYQFIENWAHRDKSYLYYDFM
                     SRDFFKWMADQDTSKEYWKAPGSGQYVYGKGLFQYKAKRCYNISLEAIEHEMATPKRE
                     WSAKQKWREIYGSTFPD"
     gene            complement(2636..3094)
                     /locus_tag="ACHKXP_005087"
     CDS             complement(2636..3094)
                     /locus_tag="ACHKXP_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7649872.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRHNIICTECRKSEVMQRILSFYQGDVRYFFEVTRLKCISSDVN"
     gene            complement(3135..>3875)
                     /locus_tag="ACHKXP_005088"
     CDS             complement(3135..>3875)
                     /locus_tag="ACHKXP_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO7649873.1"
                     /translation="AERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDT
                     DQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARP
                     EQSHQLQQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKS
                     AFSAWKARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWL
                     LPVSGTNNQRCVNHISEPDTEIPPGFRG"
BASE COUNT         1030 a          971 c          754 g         1120 t
ORIGIN      
        1 tatcatggat tccaattatc gacagctggg atttttactt tcttgatctg ccccaggcga
       61 atgacatatc cccattaacc aatgtccgct cctagcactt agtagacatt aatagattgt
      121 aagcgttccc gtcatgtagc ggtcaccgat tggtgatgaa ttgaagcgga tcattgatga
      181 aagtcgcaat aacgtggcca gcccatttat cgttcaccag atcccggaac ggcaggttaa
      241 acgcagcaaa gaagtttctc atcccactca agttgcacca gactatttga gtcggtcgtt
      301 ttcagctacg cgtgacaagc tgggtttata tgacaaacgg ccgatggatg aaaggccaac
      361 ctttcacgaa attcgcgcct tagcagcaca tctttttgat cagcagggca tcgatccaca
      421 aggacgaatg gcacacagcg atgcgaaatc aactaaaatc tacacgcaaa accatatcga
      481 ctgggttgta gttccacatg gagaaattaa agccagctag caccaagtaa ggcatagaat
      541 tttattaacc aaggagcata tgaccagtaa ttaattctta ccggaaagtt agccctgcta
      601 aaattaatat taacaaataa aaaaataggg gcaaatattt atatttgccc tcgcaacata
      661 tttatcttct tttcagttcc ttgggaagaa aagcatctat ttcttcttca gaaaatgtta
      721 aatcttcttt gttttttaat gcgtcctgtg cttttttata agcttcaaat gttgtactag
      781 gagagcctga atacactgaa tgtaaactct cgagtaacga atcccgctct gactgcaatt
      841 gctcgattgg tttttcaccc attgctaagt ctgtaagcaa tgataaatac ttttctcgga
      901 ttaaccagat ctcatttgca gcctgcttat gtttttgcgc caactcacca aggtcataat
      961 ctttggtata agcgttaaga atgagaagca gtgttgaaac aacaatccca acccccgtgc
     1021 ctacttcacc cgcgccgaaa aaagttgcta gaaagcctcc agtagttaag gcggagagaa
     1081 ttatttgcca aaactttata gaagatagtc gagataggag aatgtctgag catttttcat
     1141 gcgtcttatg tgagtaaacg acccgaccat aacactctct gatctgccct tctaaaaccc
     1201 ttctagattc aggtgtatcc ttagtctgga aatgttgatc cataaatctc ccgccatttt
     1261 tgcttagccg accattcgcg ttttggggtc gccatttcat gctctatagc ttcgagtgaa
     1321 atgttatagc accgttttgc tttatattgg aaaagccctt taccatagac atactgacca
     1381 cttcctggcg ctttccaata ctccttactc gtatcttgat cagccatcca tttgaagaaa
     1441 tcacgactca tgaaatcgta gtaaagatac gacttatctc tatgcgccca attttcaatg
     1501 aattgatacg caagcgtatc aatgagtaaa ccacttatag gtacatccca tttacgcttc
     1561 caagctcttg ccattctaca aagttgcacc aaattgttat tggtaatgtt atttcttaac
     1621 ctcattgcag aaatttctgg ctttggattt gtaatcctcc aacggccacc accgttcgca
     1681 tcaggaaatg tgtaactatc actaacattg aggaaagctg gaacgacttc aaatgtaatt
     1741 ccatcattga acggtacaag gatcacttga ccgtcacctc taatactagt ggtagcatag
     1801 gttttttcaa tagatctctt aaccgcttga agcaatgctg actgcccatt tcctaagtag
     1861 ctattgtatt tttgatactc ggaatatggt agctgaaaaa tcatatccag atcgctcaag
     1921 ccttgcgtag ctgtgttccg accatatgat ccaacgtaga gactatgaga agtatcagaa
     1981 ctgctatccc aaaaatccgt atttaaacgc ttcgttatat tcctatatct agttgaaatt
     2041 gttccaccat cttttacagt aagattggag caaaatccgc aaaaccactc agctaaaccc
     2101 atgctcaatt ccttttctaa aatctaatga aaaattcaag ggcgcatatt ccatgcatca
     2161 ttgataactt ttttcagtca gctagagcaa cataattgat actaactagc tgtttcttca
     2221 agtgagtaac ctactttagc atcaaggtat tatacctacc aagcatgggg agaaaccacc
     2281 acttaagcca ttgaagtata tagagcagat tttgttgaaa aagcactgtt tgtctataca
     2341 tatgaatatg tgcttttaac cagcaatagc gcggctctca gcgttttaca agcagtgtca
     2401 tggggtgtcg gggatcggaa gttcaaatcc tctcgtgcga cccaaaagat tatataaacc
     2461 agcctgttat tgctggtttt ttatgcctac cttcctggta gggaagctat ggggaattac
     2521 tggggaatcc ccccctttac ctgctcccat taaactaaca tagcacgatg taagcaactt
     2581 ccgcttctgg cacaaagcgg acaaccacgc tagctctacc ctgtgccaca aaatgtcaat
     2641 ttacatctga actaatgcac tttaatctcg tcacttcaaa aaaataccga acatccccct
     2701 gataaaacga caatatccgc tgcataactt cgcttttacg acactcagta caaattatat
     2761 tatgacgcct gtcgtaacga cgtatttctc catcaggtaa tgaccagata aggtccggat
     2821 caaccgcaga tggtttcttc agctttgccc ttgagagctt tttacgggca ttttgccagt
     2881 ccttacgcgc ctgttcagac gggaataacc cgtaaccaga gttgtataca tcgccactgg
     2941 caaccagctc tctggccaga acgctcatca gatatcttgt tgccccagtt ttagcttcca
     3001 gttgtcgtaa cgtctcgcgc ccactctggc gtacgagttc aagaacctgc cctttaattt
     3061 tttcccgctc ttcttgtgta aaaacttttg ccacaagccc tcctgaaaat tacctcatga
     3121 ccagaaatac acacttaccc cctgaagccc ggtggaattt cggtatccgg ttcagaaata
     3181 tgattcacac aacgctggtt gttcgtaccg cttaccggga gcaaccaggg gttttcaaaa
     3241 ttccggtccg gtccaaaaaa cgtcgtcgct cgctgaacaa attccgttcc cgttttcccg
     3301 gtagccgcca ggtatcttgc gtaacgcctc acaccatcca gcatggcctc tggtggcacc
     3361 ccctcgcgta atctggcctt ccaggcactg aaagcggatt tcttcgggtt tgccccagca
     3421 cgcaacgggt attcccgcca gacctgttcg aacacatccg gataatctac tcgccccaca
     3481 ggctgcccgg tgttttccgg gactacccga tcggcttccc gctgaatggc ggaatcggct
     3541 tcaggctgct gcagttggtg tgattgctcc ggccttgcgg tcatcacctg ctgcacagcg
     3601 cccgaatcgg ctttcagcgc atacgctgaa tcggcttccg gtgtcgtgcc tgctggctga
     3661 ccaggattga cggtctgaac atcccctgcc tggttcgtgg cgttttttac gccatggacc
     3721 atagtgtttt gatcttcttg atctgtatct ttatctgtat ctttatctgt cgtgactcgt
     3781 cgtgacatgt gcgtgacatt tcgtgacgcg ccgtgacaat cgccattttg ttcccgcttt
     3841 ctttccctct ctcgctgcgc cctcttgcgc tctgc
//
LOCUS       DAZFZC010000222         3865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFZC010000222 DAZFZC010000000
VERSION     DAZFZC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.226"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..946)
                     /locus_tag="ACHKXP_005089"
     CDS             complement(<1..946)
                     /locus_tag="ACHKXP_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7649874.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRMKTPAPVGIFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLGRLWG
                     ALPPDIRLSPHLYLATSSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPL"
     gene            complement(1014..3122)
                     /locus_tag="ACHKXP_005090"
     CDS             complement(1014..3122)
                     /locus_tag="ACHKXP_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7649875.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKDAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAESIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNITFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          863 a         1119 c          969 g          914 t
ORIGIN      
        1 ccagtggcgc accgggcagg ctttccgggt atgccgggtc gtgcatcagc cacactgccg
       61 aaaggcggat acccctgtca gggccgtatt cggtaccggg cagtgtgtcg gggaacgctg
      121 aggctgagag agggcgggaa ctgtcagaag aagatagcga agaggtgcgg gcctcttccg
      181 cacgctgcgc ctgcgtggtc agcaccagac ggaactcccg cccggcaccg tccgtcacgc
      241 ccgtgatttc cccggccagg tcaccggcgg cctcacgccg gtacgtcagc gtccgcccga
      301 agcggtctgc cagcccggta agcacccggt acggcggcag cggcgctggc agtacgtcct
      361 cagcacccgg cacccgctct gaccacccca gtatccacca cggtccctgt gcgctgctgg
      421 tcgccaagta aagatgcggg cttaaccgga tatccggcgg cagcgccccc cacagccgcc
      481 ccagcgtgtg gccgtccggc tgcgctgcct taccaccgcg caccagccac attgactcgc
      541 tgcggctgta caccgcctca cccggcagca gcggctcaaa gtgaatgctc cgtccgccgt
      601 tgtcgttgag taccagtgca tcatcgcgta tctgtaagcg gatatcagaa ggcgctttcc
      661 agccggggcc aaaaatcccc accggcgcag gcgtcttcat ccggtagctg ctgtaggtgc
      721 gggagagaat gaacggcagc gggccgggca gcgcaaggtc cgtctcgccg ggctgtactt
      781 ttgcaccgag cagcgggttc accgggttgc ccgaggtcat cccgcccgga cacaccgagc
      841 acgccacacc agttggcgca ccaattctta cacctgccga accctggaca atgggaccgc
      901 catactgagt catatctccc tgacgcgccg ctggttttcc gctcatgatt caaattcctt
      961 cttctgttta tttttaatgc tggtttgtta ttgaataccg aacaggtacg ggatcagtac
     1021 tctctcatat ccggcgtatc ctgcttttca tcaggggtct tcggtgggga tttcgaggct
     1081 gcacagttcc agttaatcaa cagtgagtca ccattaacct gctccttacc ctgcaaatga
     1141 atttttgtgc cgttgagaaa aatttgaccg tcctttgtca gcaccagcct tgccttacca
     1201 cagcagagct ccagatgctc accggcggag taaatcgcgg tctgcccggt atcatccttt
     1261 ttcgttttac caacggtgaa ggtaatgtta tttccgactt tgacgtcata acccagcccg
     1321 accctcaacg atttatgcaa accgatctgc gaggactgca tcagtgccac cgaggtgttc
     1381 ataatgccac ctaccgtcgt ctgatacgcc acgcccacgg tcagcgcacg aacaagtcca
     1441 atcgtttcca cctgaacaga gcccacttta atgatctggt tactgcccac cgtctctatc
     1501 tggttaacac ccaccgtctg tatctgattg ttggtaaccg caatcatctg attgttgcca
     1561 atgctctcag catggttgtt tatcacatcc gtggtgcggt tattcagcac ctcggtgttc
     1621 atgttcttct gcgcgtggat gtagacctgt tctttccctg tcgcatcgtc aaacttcagt
     1681 tcattaaacc cgctgccctt ataggttttc gaacgaatgg tcatctgcgt cttcgtcccc
     1741 ggcaggctgc cgggtgtgcg gttttcctgg tggtaggtac gccccataat aatcggctgg
     1801 tccggatcgc cgttgaggaa gtccacaatg acctcctgac ccacacgcgg tatcgccagg
     1861 ttgccaaagc cggtgcctgc ccacgcctgt gccacacgga tccagcatga actgtcctgg
     1921 tttgacgggt tatagcggtc ccagttaaat ttcacccgca cgcggccatg ttcatcacag
     1981 aagatttcct cgcctgccgg tcccgtcacg acagcgctct gcgggccgtc caccagcggt
     2041 ttcagcaacg gctgtggtcg ccatgttctg tctgccggga tcaccgcaaa gtgattattc
     2101 agcgtggtgc ctgaaccact gcgtcccggc accgcctgcg gctgctcgcc gtgcagttca
     2161 cttgccacca cctgccattc ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt
     2221 cgccgtcccg gccatatctc cggcgaacgg cttgttccgc gcgccacttc tgcgttgttt
     2281 cgccagccgt ccatctgcca gcgggcaaag ttctgcccgt gggcgtcctt gaaacgtccg
     2341 gggtagtcat acacttcata ctgcgtgcgc tggtagtcct ggtgctggcc ctcctggtca
     2401 aaacgcccag cccagccggg acgtttaaag gtgtagtctt tggtcactac ggaagaagga
     2461 cggatttgtg cgctgtagcg gaactggctg atgcagaagg tacttacctc ggtacgggtg
     2521 ttcgggttcc aggggatctc aaaggactcc ggcagataac gcacggtgtc gcacaggacc
     2581 aggctctggt cggtactttt ttgcgcgtgc tcctcataaa agaagatgcc ttcctccgcc
     2641 gccatccggc acaggaaatc gtaatcggtt tcgccgtact ggacacaaaa ctcacgggaa
     2701 ggatgcggct cgctgaacag cggactccac tcggtcacac cgttttcctt caggatcgta
     2761 gcgaggatgc tttcgatgtc ctcattctgg aagatacgga agttctgacg cagccctgtg
     2821 cgccacagcg gcgggcatac cttcatgctg tacagcatct ggtttttgtc gttctccccc
     2881 agttcaaacc aggtcaccac acctttcacc cggcgctgta cgtcatcgcc ctgccatacc
     2941 gtcaggtagg ccattttgtc cagcacctgc gcaaattcaa gggaaagaaa ctgctgactg
     3001 accagagaaa ggtcgaggga aaaaagtgaa gagagtgact gggtgagatg aaaggaaacc
     3061 accgcaaaag catccggtgg caggccgtcc acttcaagtg tgaaacgtaa tccggttgac
     3121 atagcaacct ccgtgaaaat gaataataat ccgcacatat tgcatgtaca acatgtcggg
     3181 cggatgaaat aagcgagtcg acatctggcg ttaccgggga atatcagaaa aatgtcatga
     3241 ttatgtgatg atttagtctg ggattttact tcagagaaac gggggtctgt ttagtcaaca
     3301 aaataatcac aaacgcaacg gatgggttct cgacgttatt cacgacaggc tcgtacatta
     3361 cagcgagtaa taaaaaacct gatatgcaaa gtcaaaccaa tattcataat gtatttttat
     3421 tcacaacaat aaataattca atcacaatca ttgaaatgaa tgatggtttg tataaggaat
     3481 atacgctacc gccccaccct gataacatga tctgcacatt gccatcttaa cgtcgttaaa
     3541 cactggctta atagtggata tttcaaatta ggaatttatt aaattcacct gatgaatatc
     3601 ataagcaaat agcatagaaa ataatgacaa ctcccccgag gtgaccattt ccaaagcact
     3661 gcaccaccac ctggtgcccg gataatcttg cttcagtggt aagagtacct gattcattgt
     3721 tcccaaaatg gtgttttccc caaccccccc aataatgtgc agctctcacg tgataatcta
     3781 ttgattaggt tatattaaga atcaatattt aaaatacatc cattttcttg aataaatcaa
     3841 catgtttatt tatcaattgc tgttt
//
LOCUS       DAZFZC010000223         3792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFZC010000223 DAZFZC010000000
VERSION     DAZFZC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.227"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1672)
                     /locus_tag="ACHKXP_005091"
                     /pseudo
     CDS             complement(1..1672)
                     /locus_tag="ACHKXP_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1618..1839)
                     /locus_tag="ACHKXP_005092"
     CDS             complement(1618..1839)
                     /locus_tag="ACHKXP_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649876.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            complement(1884..>3792)
                     /locus_tag="ACHKXP_005093"
     CDS             complement(1884..>3792)
                     /locus_tag="ACHKXP_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7649877.1"
                     /translation="TVKSFSEDERVITGIASTPSPDRDGDILEPEGAEFGSAIPFLWQ
                     HDHSRPVGQCTVRRGREGLEITATLVKPVPDMPSQLAARLDEAWAAIKTGLVRGLSVG
                     FRAHEYTFLDGGGMHFLRWELMEVSAVTVPANAECTIQTIKSFDRPFSAASGNRKPVV
                     KTASSAGASAQSKTLFHKEKSAMNIGEQIKSFENKRAALAASLEEIMNKAAEEGRTLD
                     VEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGDVVTVASAPVIRVE
                     QKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGAGTTTDPQW
                     AGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWVGE
                     GKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTD
                     FVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVTANLQPTGAVWLMSST
                     NALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGV
                     AVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVI
                     TGVNYGTASGG"
BASE COUNT          770 a         1248 c          941 g          833 t
ORIGIN      
        1 ccgtccgccg gtaccggtat tttcccgacc gcatcattca ccgcctgctg cagtacatcc
       61 ggatcatagt cacgaccatc acgcggaaca gggatatggc ttgctgcgtc cttcaccatc
      121 tgctcaagca tcggacgcac atcatcaccc gtcacgcact tctgtaatac cacagactga
      181 gaagccagtt tttcttcaaa cgtttgtgcc tgcgcggcta ttttctcctc aaatgtacgc
      241 tgtaaatccg ccagcaccat ggcgaattct tcgcccagtg cacgaataat ggacagttcc
      301 cgttccgtca ttttcgcagt atccccctga acatcgcttt caccgcatca tgctctgttt
      361 cactgattgc cttattaccg tcagatgcgc catcaggcag ttgtgctgag actgttttcc
      421 cggacgacgc gaacggatcc tcacgggcat cacgacggga cagcgcctcc agactgtagt
      481 tctgctgctg aagatacagt gcatcacctc ccgcaagggg cggcaggttc tcacgtttac
      541 gggcctcatt gggcgtcagc agcgtatttt tcaccgactc acccagcgtt ttcatgcgcc
      601 gttcgctgtc cattctcagc agcgtggtga cgtcaaactc cgtactctcg ttttcccccg
      661 tttccagcgc ctcatccagt aacagctcaa tggactcaat cagcgtctgc agacactggg
      721 aataatactg ctgctccagc gcctccacgt tatcactgga gggaggttgt cccacaccaa
      781 tcttgtaggc cgggacacgg aacaccgaac agacaatttc agccgtcatt ttcagttgtt
      841 ccaccgtctg cgcatccacc ggtgaaaacg tcgtggggtt atatttcgcc ccgttgctca
      901 gaatcgccgt tttccccgca ttttcgccgg tatacccgct gtcccagttg ctcttcagtt
      961 ttttcgcatt ttcttccgta atactgccgg ggatctcaat caccccggac ggcctgccgc
     1021 catttctgaa aaaagacgtt gaatttgcct gaatatgatg cccctgcgtg gcagccagcc
     1081 cggcagcata caccggcggc agccccacaa gcggatgaaa aaaacagtta aaccggtcgt
     1141 ggatcacttc ccgggcaggc accgtcaccg cctccgtgat cccgcagttc cggtccggcg
     1201 tgatgcgata gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg ctccagtcca
     1261 gaatacgcag ttctttgatc tgcccacggg agttacggat tttcagcacc accgtattgc
     1321 cgtgacgcag tttggcgttc agccacagtt caaaaaactg gatacgattc tgctgtgcat
     1381 tgggacgacg acagagacgg gcaatatccc cctgccgttt ttcacggcgg atcccctgtg
     1441 tatcggtctg catcaggcgc agtcgcattt tggcgatatc ctgggatatc agcgaaatgc
     1501 aagaaaacac cgcatgaaag gagaggacac tttccggatc ggctttcact ccctgctgcc
     1561 aggcaccggc aaaaggctca gccaccgcct gaaacaggct ggtccagccc acctctttta
     1621 catcacgtcc tgatttctgg ttttttcggg gtcgccgcaa aaggttccac attcgccatg
     1681 ctccgcatca cgtttctttt tctgacctgc cggacgtcgc gccgtgatgt actccgcctt
     1741 ccccaggcga accagcacct ccgcacacgg ctgtgccaca tcacggatat ccccggcccg
     1801 ggcatcatgc gtgccctgca gatactggat ttttgccatc agttactgcg ggaagctcgc
     1861 gcctcccgcc ctcctcatca gactcagccg ccggacgcag ttccgtagtt cacaccggtg
     1921 atcaccgcca ccgccgcagt acggcgacga cgccagttga tccagcgctc cgcacggatg
     1981 gccacgctgc cggtctggaa catggagacc agctccaccg gtgacggtgt gctgctgtcg
     2041 ctggtcggtt cagactgcat ctccagtgac gcttcacggg acatatccac cgccacacca
     2101 ccgtcatcag ccagataaat atccggcgca ttcaccagta ccagctggtc acccacatac
     2161 tgggagacaa tcaccggcag cccctggaag gtcccgccca gcagggtcat gtccggatat
     2221 tccttctgcc ccagcgcatt tttacgcatg gacagcgcca gggcattcgt gctggacatc
     2281 agccagaccg caccggtggg ctgcagattt gccgtcacaa actggccaaa cgcggcctcg
     2341 gcatccgcat ccggattacc gcttgacgcc gtgcccttca catcatgggt gatggacgca
     2401 ggggagacat ccgccaccgc cgcttttttc ggatcaacaa agtctgtgtc cagacgtgcc
     2461 accaccgcct ctgccagcgc attacggacc agtgcatcag cagccgggct ggaaaaacgg
     2521 atcagctctt ccgtcagtac cgcaatggcc gacaccttcg catgactgaa ggtaatggat
     2581 tcaaaatcaa acttcgtcag cggtctggcc ttgccctcac ccacccagcc tgcagctccg
     2641 ccggacacct gggcatgcac acggatattg aacggaacct gacgaagggc cgggatcccc
     2701 ccctgaccaa atcgcccgat aatggtctgc ggacgcaggt aatcaataaa gtcctgcgca
     2761 tattcctggt attcagacag gctgcctgcc cactgtggat ctgtggtggt ccccgccccc
     2821 accgccgatt tcagaacatg atgcagacgg ctgtcatccg gatactgacg gcgggccact
     2881 tccagggctt cggagcggac acctttagcc gcggccagcg atttggcaaa acgtgcgaaa
     2941 ccaatcccct tatccagttt ctgctccaca cggatcaccg gcgcagaggc caccgtcacc
     3001 acgtcaccgt taccagcctg tttcaccggc tgtgccgtgg cggccttacc ggcttccagt
     3061 tcacgcaggc gcttcaggtg agcatccacc tgacggattt ccgccgcggt gttgtcgtag
     3121 tgctcctcct cctccacatc cagcgtgcgg ccttcctctg cggctttgtt catgatctcc
     3181 tcaagggagg ctgccagcgc cgcacgcttg ttttcaaaac ttttaatctg ttcaccaata
     3241 ttcattgctg acttttcctt atgaaaaagg gtttttgact gtgccgaagc gccggcagaa
     3301 gatgctgttt tcaccaccgg tttccggttg ccggacgcgg cagaaaacgg tcggtcgaaa
     3361 gatttaatgg tctggatggt gcattccgca ttcgcaggaa cggtgacggc agatacctcc
     3421 atcagctccc agcgcagaaa atgcattccg cccccgtcca gaaaggtgta ttcatgggcc
     3481 cggaagccca cagaaagccc cctgaccagc ccggtcttaa tggctgccca tgcctcatcc
     3541 agtcgggctg ccagttgcga cggcatatcc ggtacgggct tcaccagcgt tgccgtgatt
     3601 tccagccctt ccctgccccg acgcaccgta cactggccta ccgggcggga atggtcatgc
     3661 tgccagagaa acgggatcgc actgccgaac tccgccccct ccggctccag gatgtcacca
     3721 tcccgatccg gagaaggcgt tgacgcaatc ccggtgatca cccgttcatc ctcactgaag
     3781 gatttcaccg tc
//
LOCUS       DAZFZC010000224         3759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFZC010000224 DAZFZC010000000
VERSION     DAZFZC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.229"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..217
                     /locus_tag="ACHKXP_005094"
     CDS             <1..217
                     /locus_tag="ACHKXP_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649878.1"
                     /translation="TNRPSPEAIMANYQLNEQLLEGCRPWIVIFDDVLTAGSHFKAMK
                     SLILQHIPEACILGLFVARTTRGAQII"
     gene            complement(235..2595)
                     /locus_tag="ACHKXP_005095"
     CDS             complement(235..2595)
                     /locus_tag="ACHKXP_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7649879.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPPISVQRVSV
                     LSTNEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYYHLAGTHTAGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCNRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRESGGSVFVDMNFQPYSDQWAFLASVIPMNVQNI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEGKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPQGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIARRKVNTLILVHRTELLKQWQERLAVFLQVGDNIGIIGGGKHKPCGNID
                     IAVVQSISRHGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLTRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIVADGEGLSF"
     gene            complement(2750..3313)
                     /locus_tag="ACHKXP_005096"
     CDS             complement(2750..3313)
                     /locus_tag="ACHKXP_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7649880.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLCETQSMLAGYYWVMEYTPDKGLHIHFVGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVMHYSDKSAVDNLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT         1081 a          952 c          763 g          963 t
ORIGIN      
        1 aacaaaccgg ccctcaccag aagcgattat ggcaaactac caactgaatg aacaattact
       61 ggagggatgc aggccctgga ttgttatttt tgatgacgtt cttactgccg gtagccattt
      121 taaagcgatg aaaagcctga tactacaaca tattcctgaa gcctgtatac tgggactctt
      181 tgttgcccga acaacccggg gagctcaaat tatttaaaaa gggtaaagta ctccttaaaa
      241 tgaaagccct tcaccatcag ccacaatcct gtaccccatc gccttataac cccgctggcg
      301 tttatcccac attctgagca gcactggata cgcggtatcc acaaaatcaa tgatcctgac
      361 atcgctttta ccggtatgct ctctgtgtag acgtcccgca tactgctgta atgtcccttt
      421 ccatgacaca ggcatggcaa gaatcagcgt atccagcgga gggtggtcaa atccctcacc
      481 aataagtctg cctgttgaca acaaaatacg gggagaatca ggaggaagag cattcagccc
      541 ggatatcagc atcgcacgct tttttttcga tagtcgtcca tggagaataa agggggacaa
      601 tttcagtgaa ttcatcactg atgctatctc atccagatga tctgtacgtt cagtcagtac
      661 cagaactttt cgcccctgcc cgaaagcttt catggcttct tcagctatcg ccaccgttct
      721 gtcatgatcc agagcaattt ctctgaaaat atcctggatt ctcgcatccg atggtaaatg
      781 accagatgta aaacgggaac gggtcagtac ctccagatta tgtgggcttt ccttcgggcg
      841 gaccgcagta tggcgaatgg caccacagta cataaaaata atgggatgca gaccatcccg
      901 tcggattggt gttgccgtca ggccaagcag atatctggca ttcgtttcct tcagaatcgc
      961 agaaaatgaa accgcgccaa tatgatggca ctcatccaca atgatttgcc cataattcct
     1021 gaccagaggt tcaacttctc cgtgtctgga tatggactgc accaccgcaa tatcaatatt
     1081 gccacagggt ttatgtttac ctcccccgat aataccaata ttgtcaccaa cctgcagaaa
     1141 caccgcgaga cgctcctgcc actgcttcag caattcagtc ctgtgtacca gtatcagtgt
     1201 attcactttc cgtctggcaa tcactgccgc tgcggtaact gtcttaccaa aagccgttgg
     1261 cgcacaaagc acaccagtgt cataacggag taatgccgaa acggcctctt cctgctccgc
     1321 tctgaggttt cccataaaaa cggcattaca ttccgtcccg gcaaatcgtt tatcgattaa
     1381 ttctgcagca atattgttgt ccctaaggaa agataatacg ctgtccagac atccctgggg
     1441 caacgcaatg tgttgcgggt aattctccgc acaacctata acacggggct tattccagac
     1501 tgacatacgc attgcctgag ccttataaaa ctcagggttc ggaaatgctg ccagccgaat
     1561 aagtcggtta atcagcacct gaggtaattg cgctttttcg aaatagatct ggtttgccag
     1621 cgtgattttc agcggttctg caatagaaat attcagtctg tttcctgatg attttttccc
     1681 ttcccatggc gtacccaggt cttcttcgtt gataaagttc acatccagag gatggatact
     1741 tcctgtagcc cgtaatatcg tcggttcaat attctgcaca ttcatcggga tcaccgatgc
     1801 aagaaaagcc cattgatcag aataaggctg gaaattcata tcaacaaaaa cacttccccc
     1861 cgattcacgc ggtctttttt gcagaggtaa tgctatgaga ttaccaaatc ccccttttgg
     1921 catcgtatcc tgattaggaa ataatcggtc ataagaaccc aatcgcagct gtcgagttcg
     1981 attacacgta taactaataa tagcagtccc cagacggcga gcttcgcggg ccgaaactcg
     2041 tgaagcaaaa aatatccaga catgtgctcc ctgacgtgaa cgggatattt ccagcgcagc
     2101 gggtacaccc agctcatcac aggatcgcat gaatgcggat gcatccttct gccattcagc
     2161 ttcatcaaaa tcaacagcca gaaaataaca ggaatcatct tccagcaacg gatacatccc
     2221 ggcagtatgg gtacctgcca gatggtagta gatgacgaga tcagatacag gaatcaactg
     2281 acggtgagcg cagtccccac attttatccg gggcttaccg catattctcg cctgccattc
     2341 gttagcgcag gcaggagagt accctgattt gccgctggtt ttactttccc atctaagtgc
     2401 ccatacatca tcacgcccgc gaaacaaccg acgaaataat gcaaccttct cattggtgga
     2461 taatacagaa acgcgctgca cagagatcgg cggtttacgt cgccattcaa tcccatgagc
     2521 ttcaagtaat gagaccagac gggcattttc tgccctcaat gcagctaatt catcactgtc
     2581 agatttatga accatattcg ttacctgtat ccgatccact gaggcaccac ggcagaaatt
     2641 cgggtcccct acactgatga caaagcgccc cgccctcttc tgcctattaa ctcaagcacc
     2701 tcccaacaac ttacagaaac ttaattctaa tacctaatac aactgtaatt cagttatgtc
     2761 gtggtcggcc ccggttgctt ttttcgggaa gcctgctacg gcccagaata ataccgtgtt
     2821 ctttctgatc ctgtttagcc agatagctaa gggcgtaacg taagttgtct accgcggact
     2881 tatcactata gtgcatcacg tgatcgatac gcaccgggta tttgtcttta gcccggcaaa
     2941 ggtgaaagta accctcccct tccgtgatcc gcctccagat atctcccagt tgcctggata
     3001 tctgataaga gttcttgtga cgttgcccat ccaggtagcc gacaaaatga atatgaagcc
     3061 ctttgtctgg tgtatattcc attacccagt aatatcccgc caacatcgat tgggtttcgc
     3121 aaagtaaacg gtatatttcc atacacatac tgtgcttaca ggaatgaccg aaactgggcg
     3181 tgtctttcct gtaggcaaaa tcaattctga aaggtaacag tttagaaaaa cgttgaaaca
     3241 gaccttccat gtgttcattc acgtctttca gaatcatgaa atccatttcg taattgggat
     3301 tagcattata cattttatag tccttacttt ataaaactga caggaaagtg gaatcatata
     3361 aatactgaga gtacagaaac gccgtaccgc ggcattaata aacgaggtaa ccagcagcac
     3421 agacaacctg ataaaactgt ttcgtattac ctcattacag taatagggta atgattcaga
     3481 atacagtaag actctcttaa actgagtacc agtaagaatt acagtaaaaa aaacacacct
     3541 catatgattg agtttaagaa agtaccatta aattccttac aggaagtaat ctgacattca
     3601 caaaagtcag ctatagaata gccacagaca acatcgccat taatattggc gtaccattaa
     3661 gtaaaaacag cgactctgca gagagcagcc tacagatatt taatcagtag cataaaagaa
     3721 taatgacata cagaccatac gctacacacc ataagaaga
//
LOCUS       DAZFZC010000225         3700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFZC010000225 DAZFZC010000000
VERSION     DAZFZC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.230"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..394
                     /locus_tag="ACHKXP_005097"
     CDS             2..394
                     /locus_tag="ACHKXP_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFO7649881.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            532..1416
                     /locus_tag="ACHKXP_005098"
     CDS             532..1416
                     /locus_tag="ACHKXP_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO7649882.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="ACHKXP_005099"
     CDS             complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="ACHKXP_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO7649883.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2753..3403
                     /gene="tetR(A)"
                     /locus_tag="ACHKXP_005100"
     CDS             2753..3403
                     /gene="tetR(A)"
                     /locus_tag="ACHKXP_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO7649884.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(3435..3677)
                     /locus_tag="ACHKXP_005101"
     CDS             complement(3435..3677)
                     /locus_tag="ACHKXP_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO7649885.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
BASE COUNT          686 a         1144 c         1162 g          708 t
ORIGIN      
        1 cgtgctgttc cccaactttt cgctcggcga agagtatgaa catgcgccgc cggcgacgaa
       61 tcgccaaatc tcaccgtatc tcccgagcgg acgatttcgc accggtctgc ccgtcgaagg
      121 gcttgcgatc gaacggggcg accttttcta tgcatgtccg cgagccagcg tcttttatgg
      181 cacggcgctc gacgccgacc ttcggacgcg cggcgtaagc acgcttgtca tggccgggat
      241 aagcaccacc ggcgttgttc tttcaagcgt cgcctgggct agtgatgcgg actacgacgt
      301 gcgtttggtc caggactgct gctacgaccc ggatcgggat gcccacgaag ctctgttgcg
      361 ttccgggttc ggcggacgtg tacaggtcgt gtaattttcg ggcctcgcat gatcgcggcc
      421 atagccgcag gtggattggt gcgaggcagg gccagtccaa gtcagggtgc ggtcatcgcg
      481 gcgcctgcaa ctctcaaaaa cccatcgccg tagcggaggt cttgtaatcg catgtcatta
      541 cgcacgcccg atttattgtt cacagcgata gcacctgcca tttggggcag cacctacatt
      601 gtcaccaccc aatacctgcc gaacttctca ccgatgacgg tcgcgatgct gcgggcgttg
      661 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg
      721 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg
      781 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc
      841 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct
      901 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct
      961 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc
     1021 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga
     1081 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga
     1141 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt
     1201 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc
     1261 ccggggaccg ccgtgttgct aggatggttg ttcttggatc agacgctgag tgcgcttcaa
     1321 atcatcggcg tcctgctcgt gatcgggagt atctggctgg gccaacgttc caaccgcact
     1381 cctagggcgc gtatagcttg ccggaagtcg ccttgacccg catggcatag gcctatcgtt
     1441 tccacgatca gcgatcggct cgttgccctg cgccgctcca aagcccgcga cgcagcgccg
     1501 gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc cacgttgtta
     1561 tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc aggctggtga
     1621 gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc ctggacaaca
     1681 ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg atcgggaacg
     1741 ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg gcaatcattc
     1801 cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg atcattgcct
     1861 gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg gtcgcgtccc
     1921 agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt ccgacaagtt
     1981 gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg gcccaccgga
     2041 acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct ttgtgcgact
     2101 ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc gcggcgaaga
     2161 acggagcgtg gggggagaaa ccgcccatca gcccaccgag cacaggtccc gcgaccatcc
     2221 cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg ccatcagtga
     2281 tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg gccacgatcc
     2341 gcccgatata gagaacccaa aggaaaggcg ccgtcgccat gatggcgtag tcgacagcag
     2401 cgccggccag cgagacgagc aagaccggcc gccgcccgaa acgatccgac agcgcgccca
     2461 gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc cagcagaatg ccatagtggg
     2521 cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca
     2581 ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtctgttgg
     2641 gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc ggattcttta
     2701 tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa
     2761 gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga
     2821 cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg
     2881 gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa
     2941 tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc
     3001 ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac
     3061 gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc tctgcgaggc
     3121 gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact tcactgttgg
     3181 ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg gcaccgttga
     3241 acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg aagccggtcc
     3301 ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga aaaggaggct
     3361 cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat tgatcaggac cgctgccgga
     3421 gcgcaaccca ctcactacag cagagccatg tagacaacat cccctccccc tttccaccgc
     3481 gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc cctgccctag cgtccaagcc
     3541 tcacggccgc gctcggcctc tctggcggcc ttctggcgct cctgctgcgg cgtccgctcg
     3601 tgggccgtgg cgcgggtccg cgcgccggcc tcgtgcgcct ggcgctcgcg ggcgaggtcc
     3661 agggcggccg tcttcacgtt ctgccttgcg cagatgagat
//
LOCUS       DAZFZC010000226         3638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFZC010000226 DAZFZC010000000
VERSION     DAZFZC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.231"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            315..465
                     /locus_tag="ACHKXP_005102"
                     /pseudo
     CDS             315..465
                     /locus_tag="ACHKXP_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(683..2773)
                     /gene="iha"
                     /locus_tag="ACHKXP_005103"
     CDS             complement(683..2773)
                     /gene="iha"
                     /locus_tag="ACHKXP_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO7649886.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGGSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            2712..2894
                     /locus_tag="ACHKXP_005104"
     CDS             2712..2894
                     /locus_tag="ACHKXP_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649887.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKIKIIDKTKYK"
BASE COUNT          928 a          920 c          815 g          975 t
ORIGIN      
        1 atttggtgtc cactattctc tcaggaattt caggatctgc cagacggtgc tgagacgacg
       61 cttacgattc agtgcagagg tgacctgctt tatcaaactt aattgtgaca aaatggcgat
      121 tgagaaggtg caattgactt attcggattc ggtcgtggcg aggtggtgac cctgcattct
      181 gctaaccggg ttatgagtca ggagtcagcg atagacaaag ggacttatct gaccgggccc
      241 ctggaaaaat ggcgggagag aatgatacct tcctgctgac gagaccggac tttgagatga
      301 cgagcgatag tgcggcagtg atgtacagcc tgatcggtag ctgcaaaact taacggaatc
      361 gagccggaaa cgtggctacg ccacgtgatc agtgtaatca acacctggcc tgccaaccgc
      421 atgaaagagt tgttgccttg gaacgtcact ctctctgtaa actaattttt accccacgtc
      481 cttcacggtg cgcttacact ctggacaagt atgtaaatga taagttaaaa ataatccgtg
      541 catttcctgt gacgatatta gttgttggta acgaaatctc attagcggat cggaaatgca
      601 cttaacctaa tacgttgcca ctgttccgcc aggagttgca gtggcaacgt attctaccgt
      661 cagtgatagc gttttgttat tatcagaact gatagttcag cgacatccag taatttcgct
      721 caggtatcac atatcctgtt gttgatgatc ccgtctggaa gtaatcaccg gcatacagcg
      781 tactcttacc ggcactgtac aggctcacgt cactgtaatc cttgttgagc aggttattca
      841 ccgcagcatt cagcgtcagg gcatccgtca tcttccacga cagacctgca tccaccaccg
      901 tccaggcttt caggtattct cctttctcat catacacttt cttctgtaca gcgctcagtg
      961 acgaataatt ctgggtgaaa cgtggtgttt tcccgcgata acgggcaccc agccatgatg
     1021 ccacctcttc ggtgatctgc cagttcagtt tcgcattcac catgtgttca ggggtataac
     1081 tcagcggcgc acctttgtta tcaccatcac gttgttcact tcgggtccag gtgtaattca
     1141 gtgacagcgt gacatcctct gaccacagcg gcaatgtgcc ggcaaattcc acaccgtgca
     1201 accgggcctt tccgctgttt acatagctat tggtgttatc atttatggaa taagagacaa
     1261 tcttgttgga gaagtcagtc ataaaacctg tgacattggc attcagaccg gcggggttat
     1321 cgtaatacac cccagcctca taactgacgc tctcttccgg cttcaggtcg gggttaccaa
     1381 gtagatttgt ttttccctgc ccggacacac cactaatccc tttatgtagc tgccccattc
     1441 tgggtgcctt atatcccgtg gtcacaccgc ctttcagcgt ccaggcatct gccacatccc
     1501 agaccagata tgcacgcgga ctgaagtgtc ccccgaattg ctcatgatgt tcatagcggc
     1561 tgcccgcagt cagcgcaagt gcatccgtga gatgccactc atcctcagca aataccgacc
     1621 agcttttctg ccggaaagtt tcacctgtgc tggcaaggac aactccgtct ttcatggacg
     1681 agctctgaaa ctcgcccccc accgtaacca gatgagattc tcccagaggg gtaagcagta
     1741 atgaattcag gataaggttc gattccttaa gctcccgcgg ctgaccggca agcccccatt
     1801 tgtcgcgctt cagtacactg cgtacaagct cacgaccttt attttctgtc tcgttccagt
     1861 tcagatacga tttccatgtt ccgaaggtga aagtatgatc atagccagct gaaattttgt
     1921 ttcgctcata gcgcagggtc cggtcatatc cccccgtcag actccccagt tgcccatccc
     1981 ggttatcata acgctgccgg gtggtatcca tatcaaacca gagcacatcc tgctccgacg
     2041 ccttccagtc aagacgtgca ccaagattat aattctgtga ctccgtggga taaggaatac
     2101 gcgtggctgc tgtatcgctc agtgatgtga ccgatgaacc ctgacgctgt tgtgtgctac
     2161 cgcgtacctg caggctgaca gaatcatcca caaggggacc actgctccag aaattaaact
     2221 ggctgctgtt accccatttg ttgctttcct gcagattcag ccctgcattg acggaagaga
     2281 gccatttgtc tgcattcttt ctggtaatga tattcaccac accgcccatc gcatcagagc
     2341 catacagtgt ggacatcggc cccctgataa cctcaatacg ctcaatggcg gccagagggg
     2401 gcatgaaccc ggtattcatg gcagaaaaac cgttgggagt cacgtcactg cctccgccct
     2461 gacgaacacc atcaatcagt atcagcgtgt aactggctgg cattcctcgg atgctgattt
     2521 ccagccctcc ggttttaccc gtaccacttt caacatccac accctctact gatctcagag
     2581 cctccgccag atcgtggtac tggctggact gcaattcctc ctggctaatc acagaaacac
     2641 tggcggctgc gttagtcagc tttttctcat agccggatgc cgagacaatc atcacatcct
     2701 ctgcagcagc tatgctgctg gctgaaaatc cgagacaggg aatgactacg gaagccagag
     2761 tggttattcg catattacta atctccgatc atgttaaccg gcaagacaat acacaaatac
     2821 aaacaataat gagaataatt tatatttacc ttttacaaaa aataaagatt attgacaaaa
     2881 ctaaatataa ataacgactg cctcggcatg ccagttaaca gctatccgta tgtcgtgttc
     2941 ggttttgcga ataaaaaatg gttactgtag aagcggattt ccaccacatg tcaaccatga
     3001 atggtcagtg gtgcgagacg actgggatga tttggtcgat gacttcttcc ggcgaatact
     3061 gatagaaaca ttgaaatatt gtaagagaaa gcgttcaaaa ttctgtggca cgtgaaaaat
     3121 tctacagcgt catcacctta ttcagtcgac cttcaaaata aacgtttaac agagacaatc
     3181 accatttgga ttgtccgctg aggaggcgaa caatttccta gacagcttta tcatgtcagc
     3241 ccacattgtt ggcgctgaca atactaagga caaacgatgg ggaagtagct ttttagcgtt
     3301 attttcggga gtaaaaaacg tggtatcttt ccgtatgtca gttgtatctt gctggtattc
     3361 gtagtcgtga taaggcatac aacgatattt atcttgacgt tagtgaagaa taagttgttt
     3421 tgtgatctat gttaatctgg gattacaatc cgggcttgat acatcgcatc acgactttta
     3481 ttctcttcag aatagaatat gttactcccc gctttacagg ctcaggattt gaattacaaa
     3541 ttatactttt aagtgaacaa taaccagggg tatttcttat ttcctgaaac caggcttagc
     3601 ccatttcggg atgagtttga aaaaactctt gacgcata
//
LOCUS       DAZFZC010000227         3628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFZC010000227 DAZFZC010000000
VERSION     DAZFZC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.232"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..185
                     /locus_tag="ACHKXP_005105"
     CDS             <1..185
                     /locus_tag="ACHKXP_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649888.1"
                     /translation="QNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLG
                     NMVNRLEEAFPPELPA"
     gene            261..715
                     /locus_tag="ACHKXP_005106"
                     /pseudo
     CDS             261..715
                     /locus_tag="ACHKXP_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
     gene            794..1027
                     /locus_tag="ACHKXP_005107"
     CDS             794..1027
                     /locus_tag="ACHKXP_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7649889.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDLGMHFRRIIR
                     NTEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            1127..1945
                     /locus_tag="ACHKXP_005108"
     CDS             1127..1945
                     /locus_tag="ACHKXP_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7649890.1"
                     /translation="MRLASRFGYTNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            2037..2522
                     /locus_tag="ACHKXP_005109"
     CDS             2037..2522
                     /locus_tag="ACHKXP_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7649891.1"
                     /translation="MTTVLHNSTTPSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            2538..3014
                     /locus_tag="ACHKXP_005110"
     CDS             2538..3014
                     /locus_tag="ACHKXP_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7649892.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3077..3298
                     /locus_tag="ACHKXP_005111"
     CDS             3077..3298
                     /locus_tag="ACHKXP_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7649893.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMNVTLN"
     gene            3317..>3628
                     /locus_tag="ACHKXP_005112"
     CDS             3317..>3628
                     /locus_tag="ACHKXP_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649894.1"
                     /translation="MSRIITTTVYTLNELSSTAQEKARDWYRQHHADSNWYENAYGDF
                     REVCGIFGIDLRQRVIRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARRVG
                     VY"
BASE COUNT          856 a          984 c          951 g          837 t
ORIGIN      
        1 atcagaatgc tctggagatc attttcgccg ccggtataca acaacgggcc gcgaccaata
       61 tgttaatcaa accactggtg gtcagtatca tcaggcaacg ccccgtcatg gaatatgatg
      121 catcccatct cggcaatatg gttaaccgac tggaagaagc attccccccg gagctcccgg
      181 cataacgtgt gttctgctcc ttttaggttt tatcacctga tttgccagtg tcatcgtcat
      241 gactcagaga ggtaaatacc atgattcacc tgttcaaaac ctgcattatt actggcgtca
      301 ttctggggtt aacgtggtct gccccactcc gggcacagga tcaacgttac atcggtatac
      361 gcaatacaga tacgatatgg ctcccgggaa atatttgtgc ttaccagttc cggctggata
      421 atggcggaaa cggtgaagga tttggcccct caccatcact ctgcaactca aagacaaata
      481 tggtcagacg ctggtgacca gaaaaatgga aacggaagct tttggtgaca gtaatgccac
      541 gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa aatgtcgcca caaccgaaat
      601 catcaaagca actgaagaaa gtaacggcca tcgtgtcagt ctgccgttat cggttttcaa
      661 tccccaggac taccatccac tgctgattac cgtttccgga aaaaacgtta actgatccct
      721 gaagtcagtt tcatgcttca gcctctgtca caacgcgtgg cagaggcttt ttatttatca
      781 ggagaaaaca gatatgccgg gttacacaga gtatgtcctg gctgaaggat ctttctcata
      841 cgggcaggcg gttgctgtca taacagctta ccgcaatgtg tttatccagg atgacctggg
      901 aatgcatttc cgtcggatta tccgcaacac ggaaggtcag cgacgctggc gctgccggaa
      961 ttctgaaccc gatgccggaa aggtgcttaa tacgcggctc gccagtgacg gtcttctcag
     1021 acaataaacg tcattcattt cacccatcag gccgcgtctt ctccgggaga cgtggctttt
     1081 tcatttatta caccagtaaa tcttaaccac cgataaggag caaattatgc gactagccag
     1141 tcgttttggt tatacgaacc agatacgccg tgaccgtccg ctgacacacg aagaactgat
     1201 gcactatgtt cccagtattt tcggtgaaga ccggcatact tcccgcagta aacgatatgc
     1261 gtacattccc accatcaccg tactggaaag cctgcagcgg gaaggctttc agccattctt
     1321 cgcctgccag acccgtgtgc gcgacccggg ccgccgggga tacacaaaac atatgctgcg
     1381 tctgcggcgg gccggagaga taaacggaga acatgtccct gaaatcattc tgctcaactc
     1441 ccatgacggt acctccagct accagatgct gccgggttac ttcaggttcg tctgtcagaa
     1501 cggatgcgtc tgtggtcagt ctctggggga agtgcgtgtt ccgcaccggg gaaatgtggt
     1561 ggagaaagtt attgaagggg cttacgaggt ggtgggggtt tttgaccgga ttgaggaaaa
     1621 gcgtgatgcc atgcagtcgc tgattctgcc gccaccggca cgccaggcgc tggcacaggc
     1681 ggcactgact taccgttatg gtgacgaaca tcggcccgtc accaccgccg acattctgac
     1741 gccacgacgc cgggaggatt acggtaagga cctgtggagt gcttatcaga ccattcagga
     1801 gaatatgctg aaaggcggga tttccggtcg cagtgccaga ggaaaacgta tccatacccg
     1861 tgccattcac agcatcgaca ccgacattaa gctcaaccgc gcattgtggg tgatggctga
     1921 aacgctgctg gagagtatgc gctgatgccg tttccctgaa agcgcagttc tgactcacgt
     1981 ctgtcccttc ccccgggatt tcttccattc atttactttt tataaggaat aatctcatga
     2041 caaccgtctt gcataattcc accactcctt ctgtttccgt aaccgctgca tcagggaatg
     2101 accagcctca gctggttgcc accatagtcc ctgatgaaca gcgcatcagc ttctggccgc
     2161 agcactttgg cctcattcca cagtgggtgg ccctggagcc ccgtgtcttc ggctggatgg
     2221 accgtctgtg tgaagactac tgcggtggta tctggaatct gtacaccctg aacaacggcg
     2281 gggcatttat ggcacccgaa ccggatgacg atgatgacga aacatgggta ctgttcaatg
     2341 ccatgaacgg taaccgcgct gaaatgagtc cggaagctgc cggcattgct gcctgtctga
     2401 tgacgtacag tcatcatgcc tgtcgtacgg agtgttatgc catgacggtc cattattacc
     2461 ggctgcggga ttacgccctg cagcatccgg aatgcagcgc cattatgcgc atcatcgact
     2521 gaatggagag aagtacaatg caacagcttt cctttctgcc cggagagatg acgcccggcg
     2581 agcgcagcct cattcaacgg gccctgaaaa ccctggaccg ccatcttcat gaacccggcg
     2641 tggccttcac ctccacccgt gcggcacgag aatggctgat tctgaacatg gcgggactgg
     2701 agcgtgaaga gttccgggtg ctgtatctga acaaccagaa tcagctgatt gccggtgaaa
     2761 ctctcttcac cggcaccatc aaccgcacgg aagtccatcc ccgggaagtg attaaacgcg
     2821 ccctgtacca caatgccgct gccgtggtgc tggcgcacaa tcacccgtcc ggtgaagtca
     2881 cacccagcaa ggcagaccgc cttatcacgg aacgtctggt acaggcactg ggcctggtgg
     2941 atatccgggt gccggaccat ctgatagtcg gtggcaacca ggttttctcc tttgccgaac
     3001 atggtctgct ttaacccgtc acaaccacat cacgcttgtt tgcactttta tcttctgtct
     3061 tcagaggtat cacactatga aaatcatcac ccgtggtgaa gccatgcgta ttcaccaaca
     3121 acatccggca tcccgtcttt ttcctttctg taccggcaaa taccgctggc acggcagtgc
     3181 tgaggcgtat accggtcgtg aagtgcagga tattcccggt gtgctggccg tgtttgctga
     3241 acgccgtaag gacagttttg gcccgtatgt ccggctgatg aacgtcactc tgaactgagt
     3301 cagtaagggc actctgatga gcagaattat caccaccacc gtgtatacac tgaatgagct
     3361 atcctccacc gcacaggaaa aagcccgcga ctggtaccgt cagcatcatg ctgacagcaa
     3421 ctggtatgaa aacgcgtatg gggacttcag ggaggtctgt ggcattttcg ggattgacct
     3481 ccgccagcgt gttatccggc tcagcaacgg caggtttatg gaagagccgt gcatctggtt
     3541 cagcggattc tgcagccagg gggacggagc ctgttttgaa ggacgctggc actggcagtc
     3601 agcaacagcc aggagagtcg gggtgtat
//
LOCUS       DAZFZC010000228         3608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFZC010000228 DAZFZC010000000
VERSION     DAZFZC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.233"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..322
                     /locus_tag="ACHKXP_005113"
     CDS             128..322
                     /locus_tag="ACHKXP_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7649895.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            complement(573..779)
                     /locus_tag="ACHKXP_005114"
     CDS             complement(573..779)
                     /locus_tag="ACHKXP_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649896.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLLVD"
     gene            1065..1475
                     /locus_tag="ACHKXP_005115"
     CDS             1065..1475
                     /locus_tag="ACHKXP_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO7649897.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            1766..2059
                     /gene="bor"
                     /locus_tag="ACHKXP_005116"
     CDS             1766..2059
                     /gene="bor"
                     /locus_tag="ACHKXP_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO7649898.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2150..2332)
                     /gene="rzoD"
                     /locus_tag="ACHKXP_005117"
     CDS             complement(2150..2332)
                     /gene="rzoD"
                     /locus_tag="ACHKXP_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO7649899.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2549..3046)
                     /gene="rrrD"
                     /locus_tag="ACHKXP_005118"
     CDS             complement(2549..3046)
                     /gene="rrrD"
                     /locus_tag="ACHKXP_005118"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7649900.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(3046..3261)
                     /gene="essD"
                     /locus_tag="ACHKXP_005119"
     CDS             complement(3046..3261)
                     /gene="essD"
                     /locus_tag="ACHKXP_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO7649901.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1080 a          834 c          714 g          980 t
ORIGIN      
        1 ctacgccatc aaaccacgtc aaataatcaa ttatgacgca ggtatcgtat taattgatct
       61 gcatcaactt aacgtaaaaa caacttcaga caatacaaat cagcaacact gaatacgggg
      121 caacctcatg tcaactaaga acagaacccg cagaacaaca acccgcaaca tccgctttcc
      181 taaccaaatg attgaacaaa ttaacatcgc tcttgagcaa aaagggtccg ggaatttctc
      241 agcctgggtc attgaagcct gccgtcggag actaacgtca gaaaagagag catatacatc
      301 aatccagagt gatgatggat gaacatcccg gtttctttac cttccctccc tccccgcaca
      361 gaactggcga gcgtgaggga ccaatatgtg agccataaat gccttatctt cagaaatgca
      421 gttggtatcc gaatagaacg atattgtaaa ttcacctaaa tacgaggcat tgctgtagtt
      481 gaattgcagt actgtattag cctgacagtg acagaatacg atattgactc tgtcacttgt
      541 gaaatggtca gaatggttag cagttatggt gatcagtcaa ccaacaggga ataatccttc
      601 gtattcttat cgtgctttac taacgcggcc tcaattgccc tgaatgcttc cagagatact
      661 tgatgttcta tacatgcaat tacaacatca gggtaactca tagaaatggt gctattaagc
      721 atatttttta cacgaatcag atccacggag ggatcatcag cagattgttc tttattcatt
      781 ttgtcgctcc atgcgcttgc tcttcatcta gcggttaaaa tattacttca aatctttctg
      841 tatgaagatt tgagcacgtt ggccttacat acatctgtcg gttgtatttc cctccagaat
      901 gccagcagga ccgcactttg ttacgcaacc aatactatta agtgaaaaca ttcctaatat
      961 ttgacataaa tcatcaacaa aacacaaaga ggtcagacca gattgaaacg ataaaaacga
     1021 taatgcaaac tacgcgccct cgtatcacat ggaaggtttt accaatggct caggttgcca
     1081 tttttaaaga aatattcgat caagtgcgaa aagatttaaa ctgtgaattg ttttattctg
     1141 aactaaaacg tcacaacgtc tcacattata tttactatct agccacagat aatattcaca
     1201 tcgtgttaga aaacgataac actgtgttaa taaaaggact taaaaaggtt gtaaatgtta
     1261 aattctcaag aaacacgcat cttatagaaa cgtcctatga taggttgaaa tcaagagaaa
     1321 tcacatttca gcaatacagg gaaaatcttg ctaaagcagg agttttccga tgggttacaa
     1381 atatccatga acataaaaga tattactata cctttgataa ttcattacta tttactgaga
     1441 gcattcagaa cactacacaa atctttccac gctaaatcat aacgtccggt ttcttccgtg
     1501 tcagcaccgg ggcgttggca taatgcaata cgtgtacgcg ctaaaccctg tgtgcatcgt
     1561 ttttaattat tcccggacac tcccgcagag aagttccccg tcagggctgt ggacatagtt
     1621 aatccgggaa tacaatgacg attcatcgca cctgacatac attaataaat attaacaata
     1681 tgaaatttca actcattgtt tagggtttgt ttaattttct acacatacga ttctgcgaac
     1741 ttcaaaaagc atcgggaata acaccatgaa aaaaatgcta ctcgctactg cgctggccct
     1801 gcttattaca ggatgtgctc aacagacgtt tactgttcaa aacaaaccgg cagcagtagc
     1861 accaaaggaa accatcactc atcatttctt cgtttctgga attgggcaga agaaaactgt
     1921 cgatgcagcc aaaatttgtg gcggcgcaga aaatgttgtt aaaacagaaa cccagcaaac
     1981 attcgtaaat ggattgctcg gttttattac tttaggcatt tatactccgc tggaagcgcg
     2041 tgtgtattgc tcacaataat tgcatgagtt gcccatcgat atgggcaact ctatctgcac
     2101 tgctcattaa tatacttctg ggttccttcc agttgttttt gcatagtgat cagcctctct
     2161 ctgagggtga aataatcccg ttcagcggtg tctgccagtc ggggggaggc tgcattatcc
     2221 acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat gtgcaaccga
     2281 cgacgaccag cggcaacatc atcacgcaga gcatcatttt cagctttcgc atcagctaac
     2341 tccttcgtgt attttgcatc gagcgcagca acatcacgct gacgcatctg catgtcagta
     2401 attgccgcgt tcgccagctt cagttctctg gcatttttgt cgcgctgggc tttgtaggta
     2461 atggcgttat cacggtaatg attaacagcc catgacaggc cgacgatgat gcagataacc
     2521 agagcggaga taatcgcggt tactctgttc attgctgacc ccacaaacag atttcacgct
     2581 caatctcacg acgagtcatg agacctttcc attgcttacc gccagcatat gtccagcgac
     2641 gtagctgatc acatgcgcct ttgatatcgc cctggtttat tttgcgaaga agcgtcgatg
     2701 ttctgaaatt gccagcaccc acgttgtaaa cgaatgagta aagagcgccg cgcgttgttt
     2761 ccggtatatc gactttgatg tacgggttaa tttgtctggc gacagtggca aggtctttat
     2821 tcaagagtgc tttgcattct gctttggtat acgttttacc gagcatgatg tcttttcctg
     2881 tatgcccgtg acatacagtc catacaccaa caatatcttt atatggtatg tagctgacac
     2941 cttccagacc atcgtcacca ctcggaccag tgatgagcac agacgctatg gcaacagccc
     3001 caccaccaat agcagctgct acagccttgc gtaatgatgg cgacattatt cacctctcgc
     3061 agccttacgc ttatcttctt taatcttgaa ataaaggttt gtcaggtacg tcagcaggcc
     3121 aaataccagg ctacccagca ctccaattgc cgcccactgt gagggcgtga ctttatctag
     3181 cagctgtaaa aaccagtacc cggcactacc tgctgaggtg ccataggcga cacccgttgt
     3241 taacttatcc atggatttca taaccccacc tcgcagacaa agcgggtgta aattgaggga
     3301 atactacgaa acgtaacaga ctcggagtca gtgaataact caggtattgg gttatcagct
     3361 aatatcgaga ctcaaaaaat ggaaaaaccc gctcgacggc gggtttaagc tgtgtgacga
     3421 agtaaccact cttaacagca taaccaattt tttacgtacg taaaccacta aatgatattt
     3481 gcgagaatgc taccgagtat tgaaaacacc actacaaata cataagcaaa tctcaacaaa
     3541 taaccaacaa ataatttcca gtgttatttt tagccgattt aaattgaacc ttcaaattat
     3601 agagcact
//
LOCUS       DAZFZC010000229         3561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFZC010000229 DAZFZC010000000
VERSION     DAZFZC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.234"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..305)
                     /locus_tag="ACHKXP_005120"
     CDS             complement(<1..305)
                     /locus_tag="ACHKXP_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7649902.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAE"
     gene            complement(319..1071)
                     /locus_tag="ACHKXP_005121"
     CDS             complement(319..1071)
                     /locus_tag="ACHKXP_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649903.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMAITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            complement(1079..1474)
                     /locus_tag="ACHKXP_005122"
     CDS             complement(1079..1474)
                     /locus_tag="ACHKXP_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7649904.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEALDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALSGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(1471..2046)
                     /locus_tag="ACHKXP_005123"
     CDS             complement(1471..2046)
                     /locus_tag="ACHKXP_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649905.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(2061..2414)
                     /locus_tag="ACHKXP_005124"
     CDS             complement(2061..2414)
                     /locus_tag="ACHKXP_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7649906.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(2407..2781)
                     /gene="gpFI"
                     /locus_tag="ACHKXP_005125"
     CDS             complement(2407..2781)
                     /gene="gpFI"
                     /locus_tag="ACHKXP_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7649907.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(2833..>3561)
                     /locus_tag="ACHKXP_005126"
     CDS             complement(2833..>3561)
                     /locus_tag="ACHKXP_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein E"
                     /protein_id="HFO7649908.1"
                     /translation="LNDPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     DQFEKIEVDFGRSTKNNITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGSLVLGNTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          791 a         1103 c          802 g          865 t
ORIGIN      
        1 tcttccgccc gggcaatggc atcagcaggc caggtggtga gcacctcctg ttcgatctgc
       61 atcacagcct cattcattga tggtgactcc gttttctgtg gatggttatg ccacagggac
      121 atcgccacca gaaacgcgcc ggttctgaca aggtcttcca cgctcacctg caggttaccg
      181 ctggcttcag cctgttctgc ccgtcgtttc agcagggcaa gatgctcgat acgctgcagc
      241 gcagacagct cggaaagcgt gacggacaca ccgttatatt caaattgttc ggttttcaga
      301 aacatgtatg acctccgttt accctgcagc gcccgcttca gtaacggtga cttcagctac
      361 cgtggcaaac tgaccattac cggaaatcac ggggatactc acttttccag ccttaacccc
      421 cgtcacagtg atcgccatat ctttcacagc aatggttccc gttgacggat cggcggaaac
      481 cgctctgaac gttttgtcgg ttgcactttc cggctcaaaa gaaaccgtca gggttgttgt
      541 tttccctttt gccaccgtac cagatgtcgg cgtcacctta atcgcagtga ccggcgtaat
      601 ttcgctgcgt tcttccgcca cagaaggttt gcccacgttg gtcactttca ccgtacgggt
      661 gatcacttcc ttcgccgtta cggccttacc gatactgctg acccagccac ggaacacatc
      721 caccgtgccg ttcgggaaac ggattttata ggcccgcaca tccccgcttt caaaccagcc
      781 tataagccct ttctgacctt cttctcccgg tttccaggcc agcgtaaaac tggtatctcc
      841 tgcagacttc tgcccctgcc cggtcgcagt ccagtccgcg tcttcatcat ccaggtagtt
      901 atcatcgtag ggttctgccg tcatctcgcc cggcgtcaga tcctttacct gtgccagtcg
      961 ctgccagtta tcgtctgaca acgggtttgc ataggcatca ccctgaccgt tataaaccca
     1021 cagggtggta ccggcacctt ttaccggtgc cagaggattt ggtgttggca tatcgtcctc
     1081 acatctcgta tgtaatggaa taagtcaaat cagcagaact ccataacgcc atatcgtcat
     1141 cacgacgata ctcatagccc tgcgtaacca tcgtggtaat caggcctgac agtgccggga
     1201 tcgcactcat cgccggataa atccggcttt ccatccacga atccagctct gaatccggca
     1261 cctgagcagg caggaaaact tcaatatgca gctccgcctg ccaggtatcg ctgtccagcg
     1321 cttcgcccgt gtattcagcg ccggtgagat aaacagcaat tgccggaaaa tccgcctcat
     1381 caaaaacagc ggggcgacca tcaaacagcg tcgccccgtg ttcatgctgc tcgagtgcat
     1441 ccagcactgc agcacggata tcagtatgtt tcatcgcttt atcacaatcc ttagttgttg
     1501 tttcaacgca tagcccagtt cttttggcag acgttctcgc cggatacggt taacgttctc
     1561 atcaaaagcc tgcttcagtg gggccgccat cggaatttta accacctgaa tgggaagacg
     1621 attacgcttt ttccttcctt tatcgtcatt gcccttcgca taccgggctt ctggcaaacg
     1681 ttgcataaca tgccagcgcc cattatttaa tcgctggatg aatgcccgct gataacgatg
     1741 ctgaccggct ttaagtatgc tgttcggacg atgaccaagc atcctgatcc ccagcttaat
     1801 agcagggaga tcaccgcggt taacgataat tctggcattc ggatttctga ccgtcgcccg
     1861 tttcagtctg gaccgttcct ttaccagttt ccggctcacc ctggtctccc gggcaacctg
     1921 tgatgaagac tgattaatcg ccgttgtagc cacgcggtta atagccattg ctgaagccga
     1981 cggaatggcg tttttacgaa cccggctcag attttcaatc gcctgatcaa gcccttttat
     2041 cgccataatt caccctgcgt ttatcgtcgc cggttaactg cgggtggttg cccacggttc
     2101 agccagagat aacaactgcc cccgtcatcc ggagaaacac gatccaccca gaacgtctca
     2161 ccattaatgg tcaacgtgtc accacgccgc acggcacgaa ccgtatccgt ccgcacaaat
     2221 aatgacgggc tgcttccttc aatacggatc ccactactgg caaaccccag cgactccgga
     2281 tcgtcaaaaa ccccctgaac ttcgccgcca cgctgtgcac cggaggtgaa ctgcgcactg
     2341 atgcccatca cttcaacaat cgtactgtcc accccggcaa gggcggcatc aaaggcattc
     2401 tgaaaatcac gcataaacag ccattccacc atcaacgtgt gtttttgcat ctgaggacat
     2461 aatcagaatc acccgaccaa catccgcaag ctcaacggat tcccccgttt caccatcaac
     2521 gccacagaga tggaggcagg tcagaactct gatgcgcgtt aacgcgccgg atgtttcctc
     2581 acgaacatca tgagccgcgt tttccctctc ccggatatcc atattcataa cctgtacatc
     2641 atcgccggat gactgcattt cctcttccca ttctgccacc cgctgcgcta tctctgcggc
     2701 actcccggat atatccggct cacgccccag aatcagggcc agttcatcaa gccgtttcag
     2761 attttgctct ttcgttgcca tatcatcccc ctgtgaaaaa agacacgggg gcatttcgcc
     2821 cccgctcacg gattatttca cctgtaccac cacaaactca tccgggtccg gcaacaccat
     2881 cagcggcgcg gactgcgtca tggtaaattc acgggcggga tcccccaccg tcagccagtg
     2941 tttcggataa cgggaagagg ccaccacacc ttcggacaac gcctgcgcat cctgaatggc
     3001 accatagcaa cgaatgccct ctgctgccgt attccccagg accagcgagc cctcaggcag
     3061 ataacgtttt tcggtaccgt cctctgccac ataagacgtt tttgccacca caatggccag
     3121 atcgccgtaa taccctttga aggacaccac cgcgcccagg tctttcaccg ctgtttcgag
     3181 ttgagaattt gaaccgcgac gggtatccag tttttcgcgg aacaatttaa agccattcag
     3241 cagacgccag acggtaccgt ccataatggc aatattcaca agaccgctgg cctggtcgca
     3301 gtagaggtca agatcatgtg taggatcgaa cgtgtcacga tcctgttttg accactcctt
     3361 accactaccc tgagtgatgt tattcttcgt cgacctgcca aaatcgacct caattttctc
     3421 gaactggtct ccttccatcg tgtatttgcc atacagcacg gcattcaccg cctgcatttc
     3481 ttccacctgg acaatggcgt gctcttcctg tttgaggtta tcagtgatga tacgcagacg
     3541 acggtaggcc gggtcgttca g
//
LOCUS       DAZFZC010000230         3528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFZC010000230 DAZFZC010000000
VERSION     DAZFZC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.235"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..729
                     /locus_tag="ACHKXP_005127"
     CDS             <1..729
                     /locus_tag="ACHKXP_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7649909.1"
                     /translation="LNDPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEG
                     DQFDTVEVDFGRSEGNNIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDG
                     TVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAE
                     DGTEKRYLPEGTLVLGNTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPARE
                     FTMTQSAPLMVLPDPDEFVVVQVK"
     gene            781..1164
                     /gene="gpFI"
                     /locus_tag="ACHKXP_005128"
     CDS             781..1164
                     /gene="gpFI"
                     /locus_tag="ACHKXP_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7649910.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            1157..1510
                     /locus_tag="ACHKXP_005129"
     CDS             1157..1510
                     /locus_tag="ACHKXP_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7649911.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1525..2058
                     /locus_tag="ACHKXP_005130"
     CDS             1525..2058
                     /locus_tag="ACHKXP_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649912.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            2055..2450
                     /locus_tag="ACHKXP_005131"
     CDS             2055..2450
                     /locus_tag="ACHKXP_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7649913.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2458..3210
                     /locus_tag="ACHKXP_005132"
     CDS             2458..3210
                     /locus_tag="ACHKXP_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649914.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            3224..>3528
                     /locus_tag="ACHKXP_005133"
     CDS             3224..>3528
                     /locus_tag="ACHKXP_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7649915.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAE"
BASE COUNT          840 a          812 c         1103 g          773 t
ORIGIN      
        1 ctgaacgacc cggcctaccg tcgtctgcgt atcatcaccg ataacctcaa acaggaagag
       61 catgccattg tccaggtgga agaaatgcag gcggtgaatg ccgtgctgta tggcaaatac
      121 accatggaag gggatcagtt tgatactgtc gaggtggatt ttggacgctc tgaaggaaat
      181 aacattgagc aggccgacgg taaaaaatgg tctgagcagg accgtgatac gtttgatccg
      241 acgcatgata ttgacctcta ctgcgatcag gccagcggtc ttgtgaatat tgccattatg
      301 gacggtactg tctggcgtct gctgaatggt tttaagctgt tccgcgaaaa actggatacc
      361 cgtcgcggct caaattcaca actcgaaacg gcagtgaaag acctgggggc ggtggtgtcc
      421 ttcaaggggt attacggcga tctggccatt gtggtggcga aaacgtctta tgtggcagag
      481 gacggtaccg aaaaacgtta tctgccggag ggcacactgg tcctggggaa tacgtcagca
      541 gagggcattc gttgctatgg tgccattcag gatgcgcagg cgttgtccga aggtgtggtg
      601 gcctcttccc gttatccgaa acactggctg actgtgggcg atccggcccg tgaattcacc
      661 atgacgcagt ccgcaccgct gatggtgctg ccggatccgg atgagtttgt ggtggtacag
      721 gtgaaataat ccgtgagcgg gggcgaaatg cccccgtgtc ttttttcaca ggaggctgag
      781 atggcaacaa aagaagaaaa tctgaatcgt cttcgtcaac tggctggcct gctggggcgc
      841 gaggcggata tgtcggggag tgctgcggat attgcgcaac gtgtgtctga gtgggaagag
      901 gagcttgctg cttcccggga gggcattatg cctggtgatg agagcgggcc tgagcaaaat
      961 cacacagacg atggtgagca gttgcacaac actgatgcta cggatgatgt taaagcggtc
     1021 cgtgtgcgga aatgcctgca tgtgatgggg tattgcccgg agacaggccg tcccgttgaa
     1081 ctgacgtacc ggggcatgcg tgttctggtg ccatcaccac tggcgacagc catgatacag
     1141 cacggaacgg ctgagcatgc gtgattttca gaatgccttt gatgctgccc ttgccggggt
     1201 ggacagcacg attgttgaag tgatggggct ctgtgcgcag ttcacctcgg gggcacagtg
     1261 tggcagcgaa gttcaggggg tttttgacga tccggagtcg ctggggtttg ccggtagcgg
     1321 ggtccgtatt gaaggaagct gcccgtcatt atttgtacgg acggatacgg ttcgtgctgt
     1381 gcggcgtggt gacacgctga ccattaatgg tgagacattc tgggtggatc gtgtttctcc
     1441 ggatgacggg ggcagttgtt atctctggct caaccgtggg caaccaccgg cagttaaccg
     1501 gcgacgataa acgcagggtg aattatggcg ataaaagggc ttgatcaggc gattgaaaat
     1561 ctgagccggg ttcgtaaaaa cgccattccg gcggcttcag caatggccat taaccgcgtg
     1621 gccacaacgg cgattaatca gtcttcgtca caggttgccc gggagaccag ggtgagccgg
     1681 aaactggtaa aggaacggtc cagactgaaa cgggcgacgg tcagaaatcc gaatgccaga
     1741 attatcgtta accgcggtga tctcccggtg attaagctgg ggatcaggat gccggggcgt
     1801 cgtccgaaca gcatactcaa agccggtcag catcgttatc agcgggcatt tattcagcga
     1861 ttaaaaaatg gtcgctggca tgtcatgcag cgtgtggccg ggaaaaaccg ttaccccatt
     1921 gatgtggtga aaatcccgat ggcggcccca ctgaaacagg cgtttgatga aaacgttgac
     1981 cgtatccggc gtgaacgcct gcccggagaa ctggcatatg cgctgaaaca acaactgagg
     2041 attgcgataa aacgatgaaa catactgata tccgtgctgc agtgctggat gcactggagc
     2101 tgcatgaaca cggggcgacg ctgtttgatg gtcgccccgt tgtttttgac gaagaggatt
     2161 ttcccgcggt cgcggtttat ctgacggatg cagagtatac cggtgaagag ctggatgcgg
     2221 atacctggcg ggccacactg catattgaag ttttcctgcc tgctcaggtg ccggattcag
     2281 agctggattc gtggatggag tcccggattt atccggcgat gtccgcgatc cctgcactgg
     2341 cagggatgat taccacgatg gttcagcagg gctatgacta tcgtcgtgat gacgatatgg
     2401 cgttatggag ctctgcagat ttgacttatt ccattacata cgagatgtga ggacgatatg
     2461 ccaacaccaa atcccctggc gccggtaaaa ggtgccggta ccactctgtg ggtttacacc
     2521 ggcaagggtg atgcttatgc aaacccgttg tcagatgatg actggcagcg actggctaag
     2581 gtgaaggatc tgacgccggg cgagatgacg gcagaatcct acgatgataa ctacctggat
     2641 gatgaagacg cggactggag cgcgaccggg cagggacaga aatctgcagg tgataccagt
     2701 tttacactgg cctggaaacc gggagaagaa ggtcagaaag ggcttatagg ctggtttgaa
     2761 agcggcgatg tccgggccta taaaatccgt tttccgaatg gcacggtgga tgtgtttcgt
     2821 ggctggatca gcagtatcgg taaggcagtg acggcgaaag aagtgatcac ccgaacagtg
     2881 aaagtgacca acgtgggcaa accttccgta gcggaagaac gcagcgaaat tacgccggcc
     2941 actgcaatta aggtgacacc gacatccggt accgtggaaa aaggaaaaac aaccaccctg
     3001 actgtttctt ttgagccgga aagtgcaacc gacaagacgt tcagagcggt ttccgccgat
     3061 ccgtcaacgg gaaccattgc tgtgaaagat atggtgatca ctgtgacggg ggttaaggct
     3121 ggaaaagtga gtatccccgt gatttccggt aatggtcagt ttgccacggt agctgaagtc
     3181 accgttactg aagcgggcgc tgcagggtaa acggaggtca tacatgtttc tgaaaacaga
     3241 acaatttgaa tataacggtg tgtctgtcac gctttccgaa ttgtctgcgc tgcagcgtat
     3301 tgagcatctt gccctcctga aacggcgtgc agaacaggca gaatccagcg gcaacctgca
     3361 ggtaagcgtg gaagatctcg tcagaaccgg cgcgtttctg gtggcgatgt ccctgtggca
     3421 taaccatccg cagaaaacgg catcaccgtc aatgaatgag gctgtgatgc agatcgaaca
     3481 ggaggtgctc accacctggc ctgcggatgc cattgcccgg gcggaaga
//
LOCUS       DAZFZC010000231         3526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFZC010000231 DAZFZC010000000
VERSION     DAZFZC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.236"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..184
                     /locus_tag="ACHKXP_005134"
     CDS             <1..184
                     /locus_tag="ACHKXP_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565137.1"
                     /note="catalyzes the formation of GDP and D-mannose from
                     GDP-D-mannose; also hydrolyzes GDP-glucose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO7649916.1"
                     /translation="FRFRVAEEELLLPDEQHDDYRWLTPDALLASDDVHVNSRVYFNN
                     DPRAVIGLNKKEVKNV"
     gene            177..1625
                     /locus_tag="ACHKXP_005135"
     CDS             177..1625
                     /locus_tag="ACHKXP_005135"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO7649917.1"
                     /translation="MSEVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGESSLLQST
                     LLRLSTLSCETPLVITNEQHRFVVAEQLRQIDQLRDNIILEPCGRNTAPAIALSAFTA
                     LKRNEQQDPILLILAADHIIRKADIFCEAIQKSKTVAEQGYIVTFGIIPEYAETGYGY
                     IEKGEPFKNSPNITDDKFFHVQQFVEKPNHSKAEEYVSTGEYLWNSGMFMFKASVYLQ
                     ELKKHRPDIYEICEYTASLSYNDLDFIRLPKHIFQDCPSESIDFAVMEKTKNCIVRPV
                     DIGWSDVGSWHSLWEISDKTQDGDVCKGDILTYNTKNNYIYSESALVAAVGVEDIVIV
                     QTKDAILVSKKTEVQDVKKIVEMLKKHERSEYISHREEFRPWGKFDAIDQGERYKVKK
                     IVVKPGEGLSLRMHHHRSEHWIVLSGTAKVTLNDKTVLLAANESIYIPLGATYSLENP
                     GIIPLNLIEVSSGDYLGEDDIVRQKERYRIDD"
     gene            1629..3002
                     /gene="cpsG"
                     /locus_tag="ACHKXP_005136"
                     /gene_synonym="manB"
     CDS             1629..3002
                     /gene="cpsG"
                     /locus_tag="ACHKXP_005136"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO7649918.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGGLVRDRMAAFPASGEINRKLAQPVEAINR
                     VEQHFSREALAVDHTDGISMTFADWRFNLRSSNTEPVVRLNVESKAAPKLMEEKTELL
                     LKILMRD"
     gene            3030..>3526
                     /gene="wzx"
                     /locus_tag="ACHKXP_005137"
     CDS             3030..>3526
                     /gene="wzx"
                     /locus_tag="ACHKXP_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032172824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O80 family O-antigen flippase"
                     /protein_id="HFO7649919.1"
                     /translation="MSKFIILFARGYEVLIAFCLLKLMSDFLSPLEYAQLNLFVAITQ
                     GIALFFISPLQNWILVNNDNIACEIGLNSTILFEFIYSSAISVVACLLLIISGTHHEL
                     INAGILYLFILAVITPVLSQTVIPILNLQGKIIQFAGLSILGATLGIIIPVSFVSLYK
                     DDFEA"
BASE COUNT         1030 a          707 c          806 g          983 t
ORIGIN      
        1 ttttcgcttc agagtcgctg aagaagagct gttactgccg gatgagcagc atgacgatta
       61 ccgctggctg acgccggacg cgctgctcgc cagtgacgat gttcacgtta atagccgtgt
      121 ttattttaat aatgacccac gtgcagtcat tggcttaaat aaaaaggaag tcaaaaatgt
      181 ctgaagtacc tttgattgct gtcgtaatgg ctggtggtac gggtagccgc ctttggcctc
      241 tgtctagaga acactatcct aagcaatttt tgcaactatc aggtgagagc tctttattgc
      301 aatcaacttt attaagactg tcaactcttt cctgcgaaac tccattagta ataacaaatg
      361 agcaacatcg ctttgtggtt gcagaacaat tacgtcaaat tgatcaatta agagataata
      421 ttattcttga gccatgtgga agaaatacag ctcccgctat agctctttca gcatttactg
      481 ctttgaaacg taatgaacaa caggatccta ttctgttgat tctcgctgct gaccatatta
      541 taagaaaagc ggatattttt tgtgaagcta ttcaaaaatc gaaaactgta gctgaacagg
      601 gatatattgt aacctttggg attatacctg aatatgcgga aacaggttat ggttatatag
      661 aaaaaggaga accttttaaa aattcaccaa atattactga tgataaattt tttcatgttc
      721 aacaatttgt tgaaaaacct aaccattcga aggcagaaga atatgtttcc acaggagaat
      781 atctctggaa tagcggaatg tttatgttta aagcatcagt ttatttacaa gaattgaaaa
      841 aacacaggcc tgatatatac gaaatttgtg agtatacagc ttcattatcc tataacgatc
      901 ttgatttcat acgattgcct aaacatattt tccaagattg tccgtcggaa tcaattgatt
      961 ttgcagttat ggaaaaaaca aaaaattgca tagttcgtcc agtagacatt gggtggagtg
     1021 atgtaggctc ttggcattct ttgtgggaga ttagtgataa aacgcaggac ggagatgtct
     1081 gtaaaggtga tattctaact tataacacta aaaacaatta tatatattct gaatcagccc
     1141 tagtggctgc tgtaggtgta gaagatatag ttattgttca aacaaaagat gctattctcg
     1201 tgtccaaaaa aactgaggtt caggatgtga aaaaaatagt agaaatgctt aagaaacatg
     1261 aacgctcaga gtatatatcg catcgagagg agtttcggcc ttggggaaaa tttgatgcga
     1321 tagatcaggg ggaacgatat aaagtcaaaa agatagttgt gaaacccggc gaaggactgt
     1381 ccttacgaat gcaccatcat cgttctgagc attggattgt tctttccggt acagcaaaag
     1441 taactttaaa cgataaaaca gtattgcttg ctgcaaatga atcaatctac attcctctag
     1501 gagcaacgta tagtcttgaa aatccaggaa taatccctct aaatcttatt gaagttagtt
     1561 caggcgatta tttgggtgag gatgatattg tacggcaaaa agaacgctac agaatagatg
     1621 actaatatat gaaaaaatta acctgcttta aagcctatga tattcgcggg aaattaggcg
     1681 aagaactgaa tgaagatatt gcctggcgta ttgggcgtgc ctatggcgaa tttctcaaac
     1741 cgaaaaccat tgtgttaggc ggtgatgtcc gcctcaccag cgaaacctta aaactggcgc
     1801 tggcgaaagg tttacaggat gcgggcgtcg atgtgctgga tatcggtatg tccggcaccg
     1861 aagagatcta tttcgccacg ttccatctcg gcgtggatgg cggcattgaa gttaccgcca
     1921 gccataatcc gatggattat aacggcatga agctggtgcg agaaggggct cgcccgatca
     1981 gcggtgatac cggactgcgc gacgtccagc gtctggctga agccaacgac tttcctcccg
     2041 tcgatgaaac caaacgcggt cgctatcagc aaatcaacct gcgtgacgct tacgttgatc
     2101 acctgttcgg ttatatcaac gtcaaaaacc tcacgccgct caagctggtg atcaactccg
     2161 ggaatggcgc agcgggtccg gtggtggacg ccatcgaagc ccgctttaaa gccctcggcg
     2221 cacctgtgga attaatcaaa gtgcacaaca cgccggacgg caatttcccc aacggtattc
     2281 ctaacccgct actgccagaa tgccgcgacg acacccgcaa tgcggtcatc aaacacggcg
     2341 cggatatggg cattgccttt gatggcgatt ttgaccgctg cttcctgttt gacgaaaaag
     2401 ggcagtttat cgagggctac tacattgtcg gcctgctggc agaagcgttc ctcgaaaaaa
     2461 atcccggcgc gaagatcatc cacgatccac gtctctcctg gaataccgtt gatgtggtga
     2521 ctgccgcagg tggcacgccg gtgatgtcga aaaccggaca cgcctttatt aaagaacgta
     2581 tgcgcaagga agacgccatc tacggtggcg aaatgagcgc ccaccattac ttccgtgatt
     2641 tcgcttactg cgacagcggc atgatcccgt ggctgctggt cgccgaactg gtgtgtctga
     2701 aaggaaaaac cttgggcgga ctggtgcgcg accggatggc ggcgtttccg gcaagcggtg
     2761 agatcaaccg caaactggcg caacccgttg aggcgattaa ccgcgtggaa cagcatttta
     2821 gccgtgaggc gctggcggtg gatcacaccg atggcatcag catgaccttt gccgactggc
     2881 gctttaacct gcgctcctcc aacaccgaac cggtggtgcg gttgaatgtc gaatcgaaag
     2941 cggctcctaa attaatggaa gaaaaaacgg aactgctttt aaaaatatta atgagggact
     3001 gatatttttt aattattaga atattgcata tgtcaaagtt tataatcctt tttgcaagag
     3061 gctatgaagt tcttatagca ttttgtttat taaaactcat gtcggacttt ttgtctccgc
     3121 ttgaatatgc gcaattaaat ctttttgtgg caataactca aggtattgcc ttgtttttta
     3181 tatcacctct tcagaattgg atattagtaa ataatgataa tatcgcatgt gagatagggt
     3241 taaattcaac aatattattt gaatttatat actcatccgc aatatcggtt gttgcgtgtt
     3301 tattgttaat tatatcaggt acacaccatg aattaataaa tgctggtata ttatatcttt
     3361 ttatcttggc tgtaataaca cctgtgttat ctcaaacagt tataccgatc cttaatttac
     3421 aaggaaaaat aatacaattc gcagggttat cgattttggg tgctactttg ggaattataa
     3481 ttccagtgtc ttttgtaagc ttatataaag atgattttga agcatg
//
LOCUS       DAZFZC010000232         3510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFZC010000232 DAZFZC010000000
VERSION     DAZFZC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.237"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHKXP_005138"
     CDS             <1..375
                     /locus_tag="ACHKXP_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7649920.1"
                     /translation="AAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLTGAEYTGE
                     ELDSDTWQAELHIEVFLPAQVPDSELDAWVESRIYPVMSDIPALSDLITSMVTSGYDY
                     RRDDDAGLWSSADLTYVITYEM"
     gene            383..1123
                     /locus_tag="ACHKXP_005139"
     CDS             383..1123
                     /locus_tag="ACHKXP_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7649921.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            1139..1561
                     /locus_tag="ACHKXP_005140"
     CDS             1139..1561
                     /locus_tag="ACHKXP_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7649922.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVTDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            1543..1977
                     /locus_tag="ACHKXP_005141"
     CDS             1543..1977
                     /locus_tag="ACHKXP_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7649923.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            1970..>3510
                     /locus_tag="ACHKXP_005142"
     CDS             1970..>3510
                     /locus_tag="ACHKXP_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7649924.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYKRLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAES"
BASE COUNT          810 a          871 c         1136 g          693 t
ORIGIN      
        1 gcagccgtac tggatgcact ggagaagcat gacaccgggg cgacgctttt tgatggtcgc
       61 cccgctgttt ttgatgagga agattttccg gcaattgccg tttatctcac cggcgctgaa
      121 tacacgggcg aagagctgga cagcgatacc tggcaggcgg agctgcatat tgaagttttc
      181 ctgcctgctc aggtgccgga ttcagagctg gatgcgtggg tggagtcccg gatttatccg
      241 gtgatgagtg atatcccggc actgtcagat ttgatcacca gtatggtgac cagcggctat
      301 gactatcggc gcgacgatga tgcgggcctg tggagttcag ccgatctgac ttatgtcatt
      361 acctatgaaa tgtgaggacg ctatgcctgt accaaatcct gtaatgccgg tgaaaggggc
      421 cgggaccacc ctgtgggttt ataaggggag tggtgaccct tatgcgaatc cgctttcaga
      481 cgttgactgg tcgcgtctgg caaaagttaa agacctgacg cccggcgaac tgaccgctga
      541 gtcttatgac gacagctatc tcgatgatga agatgcagac tggactgcga ccgggcaggg
      601 gcagaaatct gccggagata ccagcttcac gctggcgtgg atgcccggag agcaggggca
      661 gcaggcgctg ctggcgtggt ttaatgaagg cgatacccgt gcctataaaa tccgcttccc
      721 gaacggcacg gtcgatgtgt tccgtggctg ggtcagcagt atcggtaagg cggtgacggc
      781 gaaggaagtg attacccgca cggtgaaggt caccaatgtg ggacgtccgt cgatggcaga
      841 agatcgcagc acggtgacgg cgacaaccgg catgacggtg acacccgcca gtgcttccgt
      901 agtgaaaggg cagagcacca cgctgaccgt ggcattccag ccggatggcg caaccgacaa
      961 gagcttccgt gcggtgtctg cggataaaac aaaagccacc gtgtcggtca gtggtatgac
     1021 catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt ccggtcgtat ccggtaatgg
     1081 tgagtttgct gcggttgcag aaatcaacgt caccgccagt taatccggag agtcagcgat
     1141 gttcctgaaa accgaatcat ttgaacataa cggtgtgacc gtcacgcttt ctgaactgtc
     1201 agccctgcag cgtattgagc atctcgccct gatgaaacgg caggcagaac aggcggagtc
     1261 agacagcaac cggaagttta ctgtggaaga cgccatcaga accggcgcgt ttctggtggc
     1321 gatgtccctg tggcataacc atccgaagaa gacaaagctg ccttccatga atgaagccgt
     1381 taaacagatt gagcaggaag tgcttaccac ctggcccaca gaggcaattt ctcatgctga
     1441 aaacgtggtg taccggctgt ccggtatgta tgagtttgtg gtgactgatg ctcctgaaca
     1501 ggcagaggac gccgggcctg cagagcctgt ttctgcggga aagtgttcga cggtgagctg
     1561 agttttgccc tgaaactggc gcgtgagatg gggcgacccg actggcgcgc catgcttgcc
     1621 gggatgtcat ccacggagta tgccgactgg caccgctttt acagtaccca ttattttcat
     1681 gatgttctgc tggatatgca cttttccggg ctgacgtaca ctgtgctcag cctgtttttc
     1741 agcgatccgg agatgcatcc gctggatttc agtctgctga accggcgcga ggctgacgaa
     1801 gagcctgaag atgatgtgct gatgcagaaa gcggcagggc ttgccggagg cgttcgtttt
     1861 ggcccggacg ggaatgaagt tatccccgct tccccggatg tggcggacat gacggaggat
     1921 gacgtaatgc tgatgacagt atcagaaggg atcgcaggag gagtccggta tggctgaacc
     1981 ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc agatttgacg agcagatggc
     2041 cagagtcagg cgtcattttt ccggtacgga aagtgatgcg aaaaaaacag cggcagtcgt
     2101 tgaacagtcg ctgagccgac aggcactggc tgcacagaaa gcggggattt ccgtcgggca
     2161 gtataaagcc gccatgcgta tgctgcctgc acagttcacc gacgtggcca cgcagcttgc
     2221 aggggggcag aatccctggc tgatcctgct gcaacagggt ggtcaggtta aggactcctt
     2281 cggcgggatg atccccatgt tcagggggct tgccggcgcg atcaccctgc cgatggtggg
     2341 ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat gcctggtatc agggcaactc
     2401 aaccctgtcc gatttcaaca aaacgctggt cctttccggc aatcaggcgg gactgacggc
     2461 agatcgtatg ctggtcctgt ccagagccgg gcaggcggca gggctgacgt ttaaccagac
     2521 cagcgagtca ctcagcgcac tggttaaggc gggggtaagc ggtgaggctc agattgcgtc
     2581 catcagccag agtgtggcgc gtttctcctc tgcatccggc gtggaggtgg acaaggtcgc
     2641 tgaagccttc gggaagctga ccacagaccc gacgtcggga ctgacagcga tggcacgtca
     2701 gttccataac gtgacggcgg agcagattgc gtatgttgct cagttgcagc gttccggaga
     2761 tgaagccggg gcattgcagg cggcgaacga ggccgcaacg aaagggtttg atgaccagac
     2821 ccgccgcctg aaagagaaca tgggcacgct ggagacctgg gcagacagga cagcacgggc
     2881 attcaaatcc atgtgggatt cggtgttgga tattggtcgc ccggacactg cccagggaat
     2941 gctggagaaa gcagaaaagg cttttgatga ggcggacaaa aaatggcagt ggtatcagag
     3001 ccggagccac cggcgcggta aaacctcagc atttcttgcc aatctccggg gagcatggga
     3061 ggacagagcg aatgcgcaac ttgggctttc agccgccacg ttgcaggccg atcttgaaaa
     3121 ggccagagag atggcagcaa aggactgggc cgagtctgag gcatcacggc tgaaatatac
     3181 cgaagaggcg cagaaggctt acaaacggct gcagacgccg ctggagaaat ataccgcccg
     3241 tcaggaagaa ctgaacaagg cactgaaaga cgggaaaatc ctgcaggcgg attacaacac
     3301 gctgatggcg gcggcgaaaa aggattatga agcgacgctg aaaaagccga aacagtccgg
     3361 cgtgaaggtg tctgcgggcg atcgtcagga agacagtgct catgctgccc tgctgacgct
     3421 tcaggcagaa ctccggacgc tggagaagca tgccggagca aatgagaaaa tcagccagca
     3481 gcgccgggat ttgtggaagg cggagagtca
//
LOCUS       DAZFZC010000233         3449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFZC010000233 DAZFZC010000000
VERSION     DAZFZC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.238"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1695
                     /locus_tag="ACHKXP_005143"
     CDS             <1..1695
                     /locus_tag="ACHKXP_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7649925.1"
                     /translation="EAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAK
                     WDTPKVVKGVSFMLRLTVVADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRAVNAW
                     GQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPRLAVYDPTVQFEFWFSETRITDI
                     RQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGY
                     LDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAA
                     IQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRI
                     AMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNA
                     DISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITV
                     RISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTD
                     AQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISALLVVVMKKSTAGISIS"
     gene            1762..2361
                     /locus_tag="ACHKXP_005144"
     CDS             1762..2361
                     /locus_tag="ACHKXP_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7649926.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2426..>3449
                     /locus_tag="ACHKXP_005145"
     CDS             2426..>3449
                     /locus_tag="ACHKXP_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7649927.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESLQSAAEAELSRKTAESAAGNAARDATTA
                     AEKARESAESAQSAEQSRIAAEDAVNRIPTVVGPPGPEGEQGPAGPQGPKGDKGERGD
                     TGPVGATGERGPAGDAGPAGPQGPKGDRGERGETGLTGSTGPQGPKGDTGAAGPAGPQ
                     GPKGETG"
BASE COUNT          864 a          842 c         1079 g          664 t
ORIGIN      
        1 gaagccatcg tggataacgg ggccagcttt gagccgcagt caggcaccct gaacagcgtc
       61 atccctccgg cagtgcagca cctgacggtg gaggtgagcg cagctgacgg tcagtatctg
      121 gcgcaggcga aatgggacac gccgaaggtg gtgaagggcg tgagctttat gcttcgcctg
      181 accgtggtcg cggatgacgg cagtgagcgg ctggtcagca cggcccggac gacggaaacc
      241 acataccgct tcacgcaact ggcgccgggg aactacaggc tgacagtccg ggcggtaaat
      301 gcgtgggggc agcagggcga tccggcgtcg gtatcgttcc ggattgccgc accggcagcg
      361 ccgtcacaga ttgagctgac accgggctat tttcagataa cagcggtccc gcgtcttgcg
      421 gtgtatgacc cgacggtaca gtttgagttc tggttttcgg aaacgcggat taccgatatc
      481 aggcaggttg aaaccacagc ccgctacctt ggcacggggc tgtactggat agccgccagt
      541 atcaatatca aaccgggcca tgattattac ttttatatcc gcagtgtgaa caccgttggc
      601 aaatcggcat ttgtggaggc tgttggccag ccgagtgatg atgcatccgg ctatctggat
      661 tttttcaaag gagagatagg gaaaacccat ctggctcagg agttgtggac tcagattgat
      721 aacggtcagc ttgcgcctga cctggcggaa atcagaacgt ccatcacgga tgtcagtaat
      781 gaaatcacgc agaccgtcaa taagaaactg gaagaccaga gtgcagcgat ccagcagata
      841 cagaaggttc aggttgatac aaataataat ctgaacagca tgtgggctgt gaagctgcaa
      901 cagatgaagg acggacgcct ttatattgcg ggtatcggtg ccggtattga gaatacgcca
      961 gcaggaatgc agagtcaggt gctgctggcg gcagacagga ttgcgatgat taatcctgcg
     1021 aatggcaaca caaagccgat gtttgttggt cagggcgatc agatattcat gaacgacgtg
     1081 ttcctgaaac gcctgacggc tcccaccatt accagcggcg gtaatcctcc ggcattttcc
     1141 ctgacaccgg acggaaagct gaccgctaaa aatgcggata tcagtggcag tgtgaatgcg
     1201 aactcaggga cgctcaacaa cgtcacgatt aatgagaact gtcagattaa ggggaaactg
     1261 tcagccaacc agattgaagg cgatattgtc aaaacggtca gcaagtcttt cccccgcacg
     1321 aacagttatg ccagtggcac catcacggta agaatcagtg atgatcagaa gtttgaccgg
     1381 caggtcatga taccgccagt gttattccgc ggtggtaagc atgagaattt caacagtaat
     1441 aaccaacagt catattggta ttcaacctgc cggttaagag tgacccgcaa tggtcaggag
     1501 atttttaatc agtccacgac ggatgctcag ggcgtatttt cctcagttat agatatgcct
     1561 gccggacagg ggacgctgac actgacattc accgtatctt catcaggagc gaataactgg
     1621 acaccaacaa ccagtatcag cgctctgctg gttgtggtga tgaaaaaatc cacagcaggg
     1681 atcagtatca gctgaatttt ataacccaga acgggcgtca gaaatgacgc cttttttatt
     1741 gcagaaaagc gagaggtaat tatgcgtaaa ctttatgccg ccattttgtc tgcagccatc
     1801 tgtctggccg tatccggtgc gcctgcatgg gcgtctgagc agcaggccac gctgagcgcg
     1861 gggtatcttc atgcccggac gaacgctccc ggtagcgata atcttaacgg gattaacgtg
     1921 aaataccgtt atgagtttac ggacacactg gggctggtga cgtcattcag ctatgcagga
     1981 gacaagaatc gccagctgac ccgttacagc gatacccgct ggcatgaaga ttccgtgcgt
     2041 aaccgctggt tcagcgtaat ggcggggccg tctgtgcgcg tgaatgaatg gttcagcgcg
     2101 tatgcgatgg cgggagtggc ttacagccgt gtgtcgactt tctccgggga ttatctccgc
     2161 gtaactgaca acaaggggaa aacgcacgat gtgctgaccg gaagtgatga cggtcgccac
     2221 agcaacacgt ctctggcgtg gggggctggc gtgcagttta acccgaccga atccgtggcc
     2281 attgatattg cttatgaagg ctccggcagt ggcgactggc gcactgacgg tttcatcgtg
     2341 ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc ccgccggttc
     2401 aggcgggctt ttttgtgggg tgaatatggc agtaaagatt tcaggtgtac tgaaagacgg
     2461 cacaggaaaa ccggtagaga actgtaccat tcaactgaaa gccagacgga ccagcagcac
     2521 ggtggtggtg aacacggtgg cctctgaaaa tccggatgaa gccggtcgtt acagcatgga
     2581 cgttgagtac ggtcagtaca gcgtcattct gttggtggaa ggattcccgc cgtcacatgc
     2641 cgggaccatc accgtgtatg aagattctca accgggtacg ctgaatgatt ttctcggtgc
     2701 catgtcggag gatgacgtcc ggccggaggc actgcgtcgt tttgaactga tggtggaaga
     2761 agcggcgcgt cacgctgagg aggcgaagaa gaatgccgga gaggcggaga cgtccgcgag
     2821 gaatgccggc atatcagcca gtcaggcaga agagagcgct gcaaatgctg acacttcagc
     2881 aggggatgca tcggagtcag cccggcaggc ggcagaaagt gcagccgctg caaagcagtc
     2941 agaggaggcg tcctcgtcct cggcctctgc ggccgctcaa aaagccagtg agtcattaca
     3001 aagtgcagca gaagctgaat tgtcaagaaa gacggcagaa agtgcagccg gtaatgcagc
     3061 cagggatgca acgaccgcag cagaaaaagc ccgggagtca gcagaaagcg cacagtcagc
     3121 ggaacaaagc aggatcgcgg cggaagacgc cgtaaaccga atccccaccg tggtgggacc
     3181 tcccgggcca gagggggaac aggggcccgc gggtcctcag gggccgaagg gtgataaggg
     3241 agagcgcggt gacaccggcc ctgtcggggc aaccggcgaa cggggaccgg caggtgatgc
     3301 tggtccggca ggcccgcagg ggccgaaagg cgacagggga gagcggggag agaccgggct
     3361 gacaggaagt acaggtccac agggtccaaa gggagacacc ggggcagcag gcccggcagg
     3421 cccacaggga ccgaaaggag aaacaggtg
//
LOCUS       DAZFZC010000234         3352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFZC010000234 DAZFZC010000000
VERSION     DAZFZC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.239"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            287..496
                     /locus_tag="ACHKXP_005146"
     CDS             287..496
                     /locus_tag="ACHKXP_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7649928.1"
                     /translation="MHCYHTVEDVPLPKVVNQRYRDNHGALVTVTSVEEPRVVFMRDG
                     YPHPCMRPMYNFLGKFKPEPREETE"
     gene            complement(549..782)
                     /locus_tag="ACHKXP_005147"
     CDS             complement(549..782)
                     /locus_tag="ACHKXP_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649929.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLADNRNRLEEVVTRRL
                     QDRFYMNVSWDALNTAFSEHPEWVSGLVSGDED"
     gene            complement(878..1501)
                     /locus_tag="ACHKXP_005148"
     CDS             complement(878..1501)
                     /locus_tag="ACHKXP_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7649930.1"
                     /translation="MRTRIDYLADKYSFTELNESPRLRRQWQDVLDECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSSVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKVPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1590..2099)
                     /locus_tag="ACHKXP_005149"
     CDS             complement(1590..2099)
                     /locus_tag="ACHKXP_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649931.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            2557..3015
                     /locus_tag="ACHKXP_005150"
     CDS             2557..3015
                     /locus_tag="ACHKXP_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649932.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMIILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLVCIHSLITTIK"
     gene            3115..>3352
                     /locus_tag="ACHKXP_005151"
     CDS             3115..>3352
                     /locus_tag="ACHKXP_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFO7649933.1"
                     /translation="MEITKNKNATIKKQSSVGCFSIFTLSFSITFIPAFLTNRQELIS
                     RGLLVPALFLAEFLILVPLYFIFFRKREGLGRGSF"
BASE COUNT         1012 a          773 c          668 g          899 t
ORIGIN      
        1 acattgcaaa atttataacc ataattaatg gaaaagtgca gaaaattatt ccactgaatg
       61 cttttaatac agttggttat ctaaaaaaat atcatttttt gatttcagtc attgaaaatt
      121 gcaatgcgtg acgactcaac agcatttaag tgagtttcag gatatgggaa ggacatgaaa
      181 ggaggagcgc aataaaatac acaggaagaa ggggaaaatg tttcgtaaaa acagaaaatt
      241 aacagcgcac atcaggaaac gtcgcagtga taccacatga aatttaattc actgttacca
      301 cactgtagaa gatgttccat taccgaaagt cgtcaatcag cgatacagag acaatcatgg
      361 tgcgcttgtc accgtgacca gtgttgagga accccgggtg gtgtttatgc gggacggcta
      421 tccgcatccg tgcatgcggc caatgtacaa ctttctggga aaattcaaac ccgaaccccg
      481 ggaagaaaca gaataaaacg aaatccctgc cggcaatcac actgcctgat ggcagcataa
      541 tccatttatt aatcctcatc cccggagaca agcccggaaa cccactccgg gtgttcactg
      601 aatgccgtgt tcagcgcatc ccaggaaacg ttcatataaa acctgtcctg caatctgcgg
      661 gtaacaactt cttccaggcg attccggtta tccgccagtc cggcataacc tctctctgcc
      721 agccactcat tcagttcatg ttccagagac agattgataa tatcgtaatc ggaccttgcc
      781 attcccgtgt ccttagcaca tgttttcagg cacggggata aagtataaag aatgtcgtac
      841 tgctgctgcc agagcaatat tccccctgat ggtcgtatca gagatggtat gcaggtactt
      901 tgcgggttgt tcccgtcagg ttatcgtgca cctccacggt cgtagtcacc accggcattc
      961 cggcctttct cagcctcagg acatcagccg caatacgctg actgccagac cagaacaggc
     1021 cgtccagtgc ggtcaccagt aaccccgcct ccagcgcata cttcagccgt tcacggggca
     1081 ctttcacttc ccgggcaacc tgctgatatg gcaatgatgt gttttcattc ccaaccacgc
     1141 gacgaatacg gtgagacaga tgataccggt atgtatccgg cacactggaa agactggcct
     1201 tcaggctgta cacgcagcca aagcgtttat cgttgaacac cacatttttc tggctgatgt
     1261 cccattcttc acgcagcgcg gcaatcagtt gtggtgtacg ggtaagcagc aagcgaaaag
     1321 gcagttcaaa actggtgacg taatccacat tcagcagtgc aatacgcaac cgttcttcag
     1381 gccctgcctc tgtctgccgg cactcatcca ggacatcctg ccattgccgg cgaagacggg
     1441 gcgattcatt cagttcagta aaactgtatt tatctgcaag atagtcaatt cgtgtacgca
     1501 tactgaaagg aattccgtat aaagattcag cagacaaaac tttatcagcc agtaaaaatt
     1561 aacggaagca ttgatattcc tcccgacaat caccggatga ttgttgcaat aacctgcgac
     1621 ataaaggact gaacagccgt ttttaacgcc acgtattgcc ctgatgaacc aggccgggca
     1681 acccgaaaac atctttccac ccggcattgt ccgccagcac ttatcaccga ccaggtctgt
     1741 tgcagtaaat ccaccagttg cgcacattct tcatcagcca tgacgccgac tcgcggatgg
     1801 caaatactcg tctggctgag cagcaacaga catcgtatct gtgctccttc atgttgttcc
     1861 cgcaaccagc gtaataccac cggcgctgat gggctgaccg tgtgattatg ctccgtaata
     1921 cgttcgtgta cccgtcggtg aaaggaacta caaaatgtct gaatctgttg accatagatt
     1981 ccttctgccg aatagagcgt gaagtgaaat gccaacagat ttggccagtg gtccaccgcc
     2041 tgctgaacaa aacgccggat ttcccccggc tctgaaatta aggcttcggt tatttgcacg
     2101 aaatgttcct tttcaacccc tgttgaattt tgtcaagccg gtgcagacac agtaaagcgc
     2161 gcatcaacac ctgaaaggta cgatacaaac ataagttaca aacaataaga acggaaggac
     2221 aattaatcag tgaggaataa taactaaatt aaccaaaaga atgttactga caatactacc
     2281 aatacttaaa tagcaatata ttaattaatg taagaacaga tgcccggtaa gacatataca
     2341 caatacatcc acaaacacac tcttttcatt aaatcaatca cctgattaaa ccatgaaagt
     2401 gtgatgtaag tcaaaattac tgacactatc aattgataat gattatctct atcattatcg
     2461 tgaaattcga gaatgaaagg agaagtagtc attataagcc ttttataaat cgtcatcatc
     2521 atagtaaagt agaacacttt ttatatgagt tactatatga aaatatactg gacaagaaaa
     2581 cgcattcctg aactttcagc acttccacct tcattaagaa agaaaaattt tactgacgca
     2641 tataatgcag catcttctca tatcgaatac tggataggtg ctggcgtctc atttatctcg
     2701 atgataattt tatccagagt atatgacttt cttttacccg cccaagatac tttcccgggt
     2761 gatatcatca gaagtttatg tgttgtgtgt ccatccatat taatctggtt tcagttttcg
     2821 gtatatgtca tgaggaaata ttacagacat attctggtgc gaggaaaaga aaccgaaacc
     2881 atttcagaaa ggttgatacg tgaggctgat acacgagaat atgaactatg gcgtccggtc
     2941 cgtcgctttt tctccattgt ttttctcatt gttctattag tttgcattca ttcactgata
     3001 acaactataa aataaaagtt atggctgttc tgatacagga aaataaacgt tcattctatg
     3061 gttacctctt cctgttatat agaagaattt taaacgacaa gtaagggaat ataaatggaa
     3121 ataacaaaaa ataaaaatgc aactataaaa aaacagtcat cagtcggctg cttttcaata
     3181 tttaccttat cattttctat tacctttatt cctgcattcc ttacaaacag gcaggaactt
     3241 atatccaggg gacttcttgt tccagcatta tttcttgctg aatttctaat tcttgttcca
     3301 ctgtatttta ttttcttccg aaaaagagaa ggattaggaa gaggttcttt tc
//
LOCUS       DAZFZC010000235         3293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFZC010000235 DAZFZC010000000
VERSION     DAZFZC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.240"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..692
                     /locus_tag="ACHKXP_005152"
     CDS             3..692
                     /locus_tag="ACHKXP_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7649934.1"
                     /translation="MHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNEKRE
                     PVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVR
                     RRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDGALF
                     PGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCEMRGMVANF
                     GGFLSINKLSQ"
     gene            703..1446
                     /locus_tag="ACHKXP_005153"
     CDS             703..1446
                     /locus_tag="ACHKXP_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7649935.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMSGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSQGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLVAASACM"
     gene            1443..2024
                     /locus_tag="ACHKXP_005154"
     CDS             1443..2024
                     /locus_tag="ACHKXP_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7649936.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            2263..>3293
                     /locus_tag="ACHKXP_005155"
     CDS             2263..>3293
                     /locus_tag="ACHKXP_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7649937.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKVKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQYLASVILENLPERPFNIRMVRETADNTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCS"
BASE COUNT          723 a          821 c         1066 g          683 t
ORIGIN      
        1 atattcacga agaaagtctg aacgagtcgg ttaagtcaga gcagtcaccg cgggtggtgc
       61 tctgggaaat cgacctgacg gtgcagggcg gtgagcggta ttttttctgc aatgagctga
      121 atgaaaaaag ggagccggtg acctggcagg ggcggcaata tcaggcgtac ccgattgagg
      181 gcagtggctt tgagatgaac ggaaagggca gcagtgcccg cccgtcgctg acggtgtcca
      241 atctgttcgg tcttgtcacc gggatggcgg aggatttgca gagcctggtg ggtgccacgg
      301 tggtccgccg ccgggtgtat gcgcgttttc tggatgcggt gaactttgtg gcaggcaatc
      361 cggaggccga cccggagcag gagctgactg accggtgggt ggtggagcag atgtcatcgc
      421 tgacggccat gacggcctcg tttgtgctgg ccacaccgac cgagacggac ggggcgctgt
      481 ttcccggtcg catcatgctg gcgaacacct gtatgtggac ctaccgctct gatgagtgtg
      541 gttacacggg cggggctgtg gcggatgagt tcgataaacc caccaccgat atccgtaagg
      601 acagatgcag caagtgcatg cgcgggtgtg agatgcgcgg catggtggct aattttggcg
      661 gtttcctttc cattaacaaa ctttcgcagt aaatcccggt ttatgacaca gactgaatca
      721 gcgattctgg cgcatgcccg gcggtgtgcg cctgcggagt cgtgcggctt cgtgataagc
      781 acgccggagg gggagcggta tctgccgtgt gtgaatattt ccgcagagcc ggaggcgtat
      841 tttcgtatcg caccggaaga ctggctgcgg gcagagatgt cgggggagat tgtggcgctg
      901 gttcacagtc atcccggtgg tctgccctgg ctgagcgagg ctgaccggcg gctgcagata
      961 aaaagcgcac tgccctggtg gctggtcagc cagggtgaca ttcacaaatt ccgctgcgtt
     1021 ccgcacctga ccggacggcg ctttgaacac ggggtgacgg actgttacac gctgttccgg
     1081 gatgcatacc atctggcggg aattgatatg ccggattttg agcgtgagga tgactggtgg
     1141 cgcaacggtc agaaccttta cctggacaat atggcggtca caggctttta ccgggtgccc
     1201 ctgtcctctg cacaggcggg cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac
     1261 catgccgcca tttactgtgg caacggtgag ctgcttcacc atctgcctga acaactgagt
     1321 aaacgggaga ggtattccga aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc
     1381 cactggcacg catctgcctt cacggggatt tacaacgatt tggtcgccgc ctcagcctgt
     1441 atgtgaacac ggcagcggaa gccatccggg cgctgtcgtt acaggtgccg ggctttcgcc
     1501 gtcagatgaa cgaaggctgg taccagatac gtattgccgg ttatgacacg gcaccggagg
     1561 cggtgtacgc ccgtcttcac gaacagctgg gtgagggaac ggtcatccat attgtgccgc
     1621 gactggccgg ggccggaaag ggtggactgc agattgtgct gggggcggca gccatcgtgg
     1681 ggtcgttctt cacggccggg gcatcaatgg cgttatgggg ttcagccctg gcagccggtg
     1741 gtttttctgc caccacgatg ctgttttcac tgggggccag catgattctg ggtggtgtgg
     1801 cccagatgct ggccccgaag gcaaagacgc cggagtacaa aagcacggat aacggtaaac
     1861 agaacacgta tttttcgtca ctggacaaca tgattgccca ggggaacccg atgccggtgc
     1921 cttacggtga aatgctggtt ggctcccggc gaatctccca ggacatcagt acccgtgatg
     1981 aaggcggtga cgggaaggtg gtggttatcg ggcggcaggc ataaaacata aaaaatcccg
     2041 cagtgctcac ggacaggaac tgcgggagag ttacgaagat taactataga gagttattct
     2101 tatgtcacga caaaaaacat taacgcagag aaattattag cgccacagtc agtttgtgaa
     2161 aatgtgaaga tattcagaat ttttattcag tcatgataca ggcatcctcc gggatgcctg
     2221 ttgtttttgt gcgtaacagt tatcacagta aagggtgaga caatgggcaa aggtggcggc
     2281 agggcacaca cgccggttga ggcaaaggac aatcttaagt ccacgcagat gatgagcgtg
     2341 attgatgcga ttggtgaagg gccgattgaa ggtccggtga aggggctgca gagtatcctg
     2401 gtgaacaaaa ccccgctgac ggacacggac ggtaatcctg tgatacatgg tgtgacagcg
     2461 gtctggcgcg ccggggagca ggagcagaca ccacctgaag gctttgagtc ctccggggcg
     2521 gaaaccgcac tgggcgtgga agtgacgaag gtaaagccgg tgacgcgcac cattacgtcc
     2581 gcgaacattg accgcctgcg ggtcaccttc ggggtgcagt cactgttgga gaccacctca
     2641 aagggcgacc gtaatccctc ttctgtccga ctgctgattc agttgcagcg taacggtaac
     2701 tgggtgacgg aaaaggatgt caccattaac ggcaagacca cctcacagta cctggcgtcg
     2761 gtgattctgg agaatctgcc tgagcggccc tttaacatcc ggatggtccg ggagacagcg
     2821 gacaacacct cggaccagct gcagaataag acgctctggt cgtcatacac cgaaatcatc
     2881 gatgtgaaac agtgctaccc gaacacggcg attgtggggt tgcaggtgga tgcggagcag
     2941 tttggcggtc agcagatgac ggtgaactac catatccgcg gtcgcatcat ccaggtaccg
     3001 tcaaactatg acccggaaaa acgcacgtac agcggcatct gggacggcag cctgaaaccg
     3061 gcatacagca acaacccggc ctggtgcctg tgggacatgc tgactcaccc gcgctacggc
     3121 atgggaaaac gtctgggggc ggcagacgtg gacaaatggg cgctgtatgc cattgcgcag
     3181 tactgcgacc agacggtccc ggatggtttc gggggcacag agccgcggat gacctttaat
     3241 gcgtacctgt cacaacagcg taaggcgtgg gacgttctca gtgatttctg ctc
//
LOCUS       DAZFZC010000236         3209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFZC010000236 DAZFZC010000000
VERSION     DAZFZC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.241"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..134)
                     /locus_tag="ACHKXP_005156"
     CDS             complement(<1..134)
                     /locus_tag="ACHKXP_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7649938.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     "
     gene            complement(156..902)
                     /locus_tag="ACHKXP_005157"
     CDS             complement(156..902)
                     /locus_tag="ACHKXP_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7649939.1"
                     /translation="MKNIAAVGVLERIRRLAPQGSVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            complement(909..1871)
                     /locus_tag="ACHKXP_005158"
     CDS             complement(909..1871)
                     /locus_tag="ACHKXP_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7649940.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(1894..2319)
                     /locus_tag="ACHKXP_005159"
     CDS             complement(1894..2319)
                     /locus_tag="ACHKXP_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7649941.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(2303..2584)
                     /locus_tag="ACHKXP_005160"
     CDS             complement(2303..2584)
                     /locus_tag="ACHKXP_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO7649942.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            2685..3104
                     /locus_tag="ACHKXP_005161"
     CDS             2685..3104
                     /locus_tag="ACHKXP_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649943.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADKLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
BASE COUNT          799 a          887 c          678 g          845 t
ORIGIN      
        1 ttatcaacga cgccttttct tttgagttcc cacagctcgt tgacagcctc ttcacgactg
       61 attccaaggc gagctgccac cacatgtgaa gaggcttttt tcagtgcttt cagtgcgtca
      121 gatacggttt ccattaaaat ttcctccgga caaaattact tcacaaccct catattgctg
      181 acatttggac gccagctatc ccagttaaac gtcacccatc gaccaccgtt catggtcatg
      241 cggtccataa tcctctcacc aagaagcgta ctcattgcgg catgattcag gtttgttaac
      301 atcccgacac tgcacagtga tgctgtccgg cgatcaatta tctggtgcaa taccacctgc
      361 tcgtttttcg tctcccgctg aacgcctatt tcatccagga ccagcaaatc aaccccgcaa
      421 agctcctgta aaaatttttc cccggatttg ccgttgtcgt agctgtcatg caacacgctc
      481 atgacgtcag acacggtgac gataatcacg ctgcgcccct tcaccatcag ccggttgccc
      541 atcgccgctg caaggtgatt tttcccggtg ccggttttac cgctgaacac aaaattcgtg
      601 cacccggtca tcagttcgtc agctatggat ttggcctggc tcagcgcgta tttttgcccg
      661 tcgttctgca cctgataatt tgcaaacgag catttgctgt gcagaggctg gatgcccgaa
      721 cgattcagga ttttttccac ccgcaactgg cgattctggc ggttaatctc ctcgctgcgt
      781 tttcgtcctt cagcaagttg ccattcccgc cactcctcca ccgtccggta cggtggaacc
      841 gacccctgtg gtgcaagtct gcgaatacgt tcaagaaccc caactgccgc aatgtttttc
      901 atgacacgtc accccctgaa tcccggcggt atttcagtgt ccggttcaga aatgtgattc
      961 acgcaacgct gcgcaggcga acgccccagg cggataacca gttcatccca tttttcccgg
     1021 agttttgccg gactcatgat gttttttacc cagaacgaat cccgctggag acgcccaaac
     1081 atttcacaaa tttgtctgtg agttctgcca tccagcatcc gcattgtgcg aacgtcattg
     1141 gcccatgctg tccagttggg ttctttcggt ctagtgatct cgccatcatc gctggccgcc
     1201 tgctcgtaaa gactcacgat tcgtccccag atccactgtg cgcacaccaa atcttcctga
     1261 cttccccact ggcgtttttt cgcactgaac acaaccgcgt cagggtgtcg ggttaaaaaa
     1321 tcctgttcag ccgtctgcgg gtccggttgc gaagcgtccg gacaagaaga tcttttatct
     1381 gacggatcag gttttaatac tgacggatcg gggtcaatca tcgcccccct aatcggcagt
     1441 tttttatcaa cagttgatcc atcaaaattt gacgggtcaa ccgttgaggg gtcaatattt
     1501 gacgggtcaa ctgttaacgg gtcatttttt gccgggctaa tttttctttt cggtttatat
     1561 gactcacgcg ccgccgccgc agctgcttcg agtttttcca cattaagccg atagatattg
     1621 cttacattac gcccaccgac cttacgctct tccttcgtca gccagccctc tttcgccagt
     1681 tctgcaatag ccgatttcac tgtggattca cttcttgcac cgatctgacg ccggatagtt
     1741 tcaatggcag gccatgacac gccctcgtca ttgctgtagt ctgcaagacg ggccataacc
     1801 gccaccctgg ataagatcat gccggtgaag gcgcaccctt cccagacaag accatgaagc
     1861 ttgctgctca taaaaccccc gaacaccgtg cttttagtgc atcaccacag cattccctgc
     1921 cgggccgccg cgattcatct ggtcatacaa aacaaccgct gacgcaacaa aatcatcgac
     1981 atccttcacc agccgatccc tccgttcgac gatctcacgg taatattcag aactgtggct
     2041 gcgcatacgg gccaccagca aaggcggcat cgccttttcg atcgccggta acagagcctg
     2101 cattttttca acagcatcag gggtgtcttt ctctacccag cggaaaattt tctgggtatt
     2161 gcgagccagg gcttccggat ggctgtcgtc atacagttct gggaacgtca tacccaactc
     2221 aaaataagcc tgggttattc cagctgctgg aactttttcg ccatcaggac gcgcccaggc
     2281 attcatcgcc atgcggatgt gttcatgctt gattttcatg aatcaagctc ctagaaagtg
     2341 gttgtgttaa cgttttggta tcttccagct cgggccaaat attcatccaa tcaaaaggcc
     2401 ttagttgctg acgtgtaact tcaccattac tggctcgctc aataaggaca cataacgatg
     2461 cccctaacac ttgaccttta ctcaatgcct ttcttagata accgatgctg gtaccacact
     2521 cgcatgcaaa catacgctgt tcatctgacg aaagagaatt gagaaatatt cttaattctt
     2581 ccatagctac tccttagtaa acacagcaaa gaatacccac aggtaaacaa aagtcaatac
     2641 ccacaggttg tttaccttgc ggtaatcgca tctattattt acctatggac aaatatgaat
     2701 ttagacgaca gcaactcatc aaaattcgtg atgagaaatg cgatggtaaa gcggttaacg
     2761 tggccagaaa gatcgggcgc gagccttctt atgtatcaag aatgttgtac ccagagggga
     2821 aaaagggaaa aaaacggatc gctgatgata tggtggagat tatcgaagag tcctttgggt
     2881 taccccgggg atggatggat ggtatcgttt catcatcaac gaacacagcc tccagttatg
     2941 aaacaagggt tctaacgcca cgacaacgta tttttttaga tctcttagac gaactgccag
     3001 aaagtgaagc ggataaatta ttaaaaactc ttgaagagaa aaaacagtat tacaatatga
     3061 tctacgaaga aatccgtaaa aagaaagcac aaaacgcatc atagctcacc aaacaactag
     3121 tcaccagtta agacaccgca aaaatttacc catgggtatt tactttttaa atacctatgg
     3181 gtatccttct tttcatacca acccacccc
//
LOCUS       DAZFZC010000237         3117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFZC010000237 DAZFZC010000000
VERSION     DAZFZC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.242"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..316
                     /gene="ymgC"
                     /locus_tag="ACHKXP_005162"
     CDS             68..316
                     /gene="ymgC"
                     /locus_tag="ACHKXP_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO7649944.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            648..2171
                     /locus_tag="ACHKXP_005163"
     CDS             648..2171
                     /locus_tag="ACHKXP_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7649945.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2303..2521
                     /gene="ymgF"
                     /locus_tag="ACHKXP_005164"
     CDS             2303..2521
                     /gene="ymgF"
                     /locus_tag="ACHKXP_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO7649946.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            2921..>3117
                     /locus_tag="ACHKXP_005165"
     CDS             2921..>3117
                     /locus_tag="ACHKXP_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649947.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYATQLLDDYSIISYMTDEESPIE
                     IKDNNPISNGEYLTTEDESHAV"
BASE COUNT          861 a          616 c          659 g          981 t
ORIGIN      
        1 tttttgtctg tcgctttaga ctatacacag cataatttta ttgggttaat atttctacga
       61 ggctgacatg aataattcaa tcccagagag atttattttt caatgtgctt tgtttaaaaa
      121 tctcgagagg gaggtgttca tgacgcatgg gtatgttgat agtcatatta ttgatcaggc
      181 tttacgcttg cgtttaaaag atgaaaccag tgtgattctt tctgatctct atctgcaaat
      241 attgcagtac attgaaatgc ataagactac actaacggat atcattatta atgacaggga
      301 atccgtgctc tcttagaact tgagtaaggt tttagaataa acatcagctg tatcaccatg
      361 ctgatgcaaa gtgagcattt caggcgttat gctttcttat tatgtccgca atatcaggtg
      421 tcaagaatgg agagttctcg ctctccattc ttgacgcctg atatcccgcc taacttattt
      481 tgtactgcct acattatgct caataattaa tgggttagat caagatggag aaaattttat
      541 cttcggcgtg gatatttctt atgctctcta ccatgctctc tgtagatgcc agcgcgagcg
      601 aatgaatggc attttcatgt attaaaccct ttggccggga caagcaaatg cgcaatacac
      661 tcatacccat cctcgttgcg atatgcttgt ttattaccgg tgtcgctatt ttgaacatcc
      721 agctctggta ttccgcaaaa gcagagtacc tggcgggagc gagatatgcc gccaacaata
      781 tcaatcatat acttgaagaa gcgtcacaag cgactcaaac agcggttaac attgccggga
      841 aggaatgcaa cctcgaggag caatatcagc ttggcactga agcagctctg aaacctcacc
      901 tgcgcacaat catcattctc aaacagggaa tagtctggtg tacatccctg cctgggaatc
      961 gggtcctgtt gtctcgtatt cctgttttcc cggacagtaa tttactgttg gctccagcaa
     1021 tcgacaccgt taatagatta cctatcctgc tctatcagaa ccaatttgca gatacgcgca
     1081 ttttggttac gataagcgat cagcatattc gtggggcact taatgtaccc ttgaaagggg
     1141 taaggtatgt attacgcgtg gcggatgaca ttattggacc aacgggtgat gtgatgacgc
     1201 ttaatggaca ttatccctat accgagaagg ttcactccac aaaatatcat ttcactatta
     1261 tctttaaccc gccgccactc tttagcttct acagacttat cgataaaggc tttgggattt
     1321 taatatttat tctgttaatc gcctgcgccg ctgccttcct gcttgataga tatttcaata
     1381 aaagcgcaac gcctgaagag atcctgcgac gggcaataaa taatggggag atcgtccctt
     1441 tttaccaacc tgtggtaaat ggtcgggaag ggacattgcg gggagttgag gtgttagccc
     1501 gctggaaaca acctcacggt ggatatatat cacccgcggc atttattcca cttgctgaaa
     1561 aatccggatt aatcgttccg cttacgcaaa gcctgatgaa tcaggttgcc agacagatga
     1621 acgctatcgc gagtaaattg ccggaaggtt ttcatattgg aattaatttt agcgcttcgc
     1681 atattatttc gccgacgttt gtcgacgagt gtttaaattt ccgtgacagt tttacccgcc
     1741 gcgatttaaa ccttgttctg gaagtcaccg agcgtgagcc attgaatgtt gatgaaagtc
     1801 tggttcagcg gttgaacata ctgcatgaaa atggttttgt catcgcactg gatgatttcg
     1861 gtactggcta ctcagggctt tcttatcttc atgacctgca tattgattat atcaaaattg
     1921 atcacagttt cgttggccgc gtaaacgcag acccagaatc aacccgaatt ctggattgtg
     1981 tattggatct ggcgcgtaaa ctttcgatca gtatcgtcgc tgaaggtgtc gaaacgaaag
     2041 aacaacttga ctatctgaac caaaataata tcacatttca gcagggttat tatttctata
     2101 aacctgttac atacatcgac ctggtcaaaa ttatcctttc taaaccgaag gtgaaggttg
     2161 tggttgagtg aaaattgatc agtaaggcca tagtgcggtg taattataga cagctaatta
     2221 gctcgttgcc tcttgttact attgttcatt attttgtttg ctataattgt ttgaaagttt
     2281 tgacaggatt gccattagta gcatgaacaa tagtaataat ctggattatt tcactctcta
     2341 tatcatattt tccattgcat ttatgctgat caccctcctg gtcatcctta ttgcaaaacc
     2401 cagtaccggg ctgggagaag tgcttgtgac gataaatttg cttaatgccc ttgtttggct
     2461 ggcgatcaat ctggttaatc gattaagaga aagactcgtc aaccacaggg atcagcaata
     2521 atctttcagt ttctcactgt cagtatgcgg ctgaatgggt tgctggcagt gaacgcctgg
     2581 atcattgaag gaaaggcatt attgcgcaaa tagttgtcaa ccctggtgtt atcacggttg
     2641 tttttatata tcaccgaaat aatcctcatc gcaactatta acaattttga tgtcgaagag
     2701 ttatttgtta aacaaaatcg tcacctcaaa gtgatcaatg tcatgaaaat aaggtgaaaa
     2761 atgataatgc cgacttattt atcatttata tattgtcgct gtttatctat ttaaattttt
     2821 tgtgcttttg tttttgtctt taccctccgt tttggtaatc tgagttcata ggttatttga
     2881 ccaggaatgt ttgttaatcc aatgaatgga tggttaatct atgaaactga aaaaactccc
     2941 cgggtttagt ttgggactta ttgctctggc ggtgggtaat gcatatgcaa cacaattgtt
     3001 ggatgattat agtataattt cctatatgac tgatgaagaa tcgccgattg aaatcaaaga
     3061 taataatccg ataagtaatg gagagtatct aaccactgaa gacgaaagcc atgctgt
//
LOCUS       DAZFZC010000238         3117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFZC010000238 DAZFZC010000000
VERSION     DAZFZC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.243"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..536)
                     /locus_tag="ACHKXP_005166"
                     /pseudo
     CDS             complement(1..536)
                     /locus_tag="ACHKXP_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
     gene            complement(552..1286)
                     /locus_tag="ACHKXP_005167"
     CDS             complement(552..1286)
                     /locus_tag="ACHKXP_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO7649948.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQWLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEISELVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(1283..1615)
                     /gene="sctI"
                     /locus_tag="ACHKXP_005168"
     CDS             complement(1283..1615)
                     /gene="sctI"
                     /locus_tag="ACHKXP_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO7649949.1"
                     /translation="MSVSNMPPIDRAEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            complement(1635..1874)
                     /locus_tag="ACHKXP_005169"
     CDS             complement(1635..1874)
                     /locus_tag="ACHKXP_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFO7649950.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEVLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            complement(1888..3069)
                     /locus_tag="ACHKXP_005170"
     CDS             complement(1888..3069)
                     /locus_tag="ACHKXP_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO7649951.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDIVVTIRELTAQEPEDRTLTFN
                     QQVEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFF
                     AFYIVNASNDPQLRHIDKILVNKNRNYEILYGRDHVIYINTNSLDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRVFSWLADNFPDLQYFKLQLLDASNPRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQ
                     E"
BASE COUNT          975 a          591 c          505 g         1046 t
ORIGIN      
        1 ttttttttcg cataaaatgt atttctctct aaaagagttg atgcaagtgg aaacggcaat
       61 tgtatgtgat caacaaaatc aggaaataat aaattaaatc gttgctgcat tgccaatggt
      121 tgttgcctta tatagggtaa caacgttgaa aaaccacatg tgatgatatt gtgatagtta
      181 gcgatgccgg gtttgtatcg tgccttttca tttatttcca ccggtatagc tgataaataa
      241 tcccttagca cagcaggaac cttataaaaa aatccgcgtt ctgcaaacct ttctcgataa
      301 aagtgataac cactgaataa acagatcaat ggaaataaat tccaattgtt tatgtattaa
      361 ataagtgaac tattcagatt aaaatgttca actgaaagat tgtactgcag aacaatcatt
      421 tcattggtaa tagatctcag caccggattg ttaataggtg tattattcaa gcttactcgt
      481 tgaggatgaa tacaactaat tggatcatat ataatctttc ttagggcaag attcataacg
      541 ctctcatatg tctacttaat tttcaacctg actaaattag ttagaatggc cctatacttc
      601 cataacagcc agcaagtcgc tacggatatt aatgcaagta agatagaaac cggcatagcc
      661 ttatcataag caaaaacaag ttcgctaatt tcatatgttg gtgcttgctc aataatgtct
      721 cttcgttttg acaatacaac agaaatattt tcatattgta cgcttgcaaa actattaaca
      781 ataaatctct tgatgtcatt tatttttatt tctgggttga tatctttttc atatactgca
      841 agtacagaaa tatgaattgg taaagcagtt ttaccactat cgccattatc aacatcgtaa
      901 ctaacatgta ctctcgaaga aataatgcca tccataattt tgagagattg ctctaaccac
      961 tgctcaatag cagaatatag ccttgctttt tccgctcgtg gagatgaaac gagtgcatct
     1021 gcagggaaca tctgcgatat ttgaatatca ggcttacccg ggagattgta gatttttagc
     1081 caatccaccg cagaagcaaa atccgttggt tcaacataga ttgagaatcc agttttgcct
     1141 tgatccttct tttcagcatt aatattatgt ctttgtaaaa cagcaaggac atcattagct
     1201 tgctgttgat caagatggtt caataattcc tgctgcttgc agccgcacaa cagcaggata
     1261 aacaataata atgatatata tttcatgagc gggtcaacgt ctcaactgca gcgacaatct
     1321 ttcttgctaa tgttcccacc aatgaaacct cgacattata attaagcaca tgatcctgca
     1381 agactaataa tttttcagga gttaacccct ttttatcaga ccttaatgct tgtaacgcat
     1441 tttgctgcca attttcagtt tgtacagcat agttggcaaa agcggagatc attttatcat
     1501 ccgggttgtc aagattgaga acccggtcat ttaaatcaat cactttcgct tgctgtattt
     1561 catgagcagt tgattgctca gctctatcaa taggtggcat attgcttact gacataataa
     1621 aatccttatc cagtttagcg gaagttttgt atgattgctg aatcaagatc tttatacgct
     1681 tttacaacgt tagattgcgc atttcgatat aatgtatact cagctaatgc actttgatat
     1741 tcggcaagaa atttcgggtc ggagggattg gttgctaaaa cctccaacgc tttttcaact
     1801 tgctggttca gatcatcaac gccctgatca aattgtttgc tgatatccat aatataacca
     1861 ttccaatctg ccataagtct tcctcaatta ttcctgaaac gtccagttgc taccttgaga
     1921 cttctcgaat cggttatctc cgtagctaaa tgaactatta tttgtgtcct gatttttcaa
     1981 attcacatta aatcttacat attgtttacc ccattgttta taatattcgt caacatagtt
     2041 attaatttta tttaattcac tgtcacttaa cgtaccaatg atattgaaat acacattgtt
     2101 agctgtttta tatttttcat aagaaagacc tatcgctgac aatgtttcaa tagcctgact
     2161 ttccaataca ttatcatcta ataccgcaat acttatattg ctggcatatg gcatagtttg
     2221 tagtaaccct ttaataagat tgtcgattag ttgctgtgtg atagcattcc gttgcttact
     2281 cacggtcagt ctgggattac tggcatctaa tagctgaagt ttaaaatatt gtaaatcagg
     2341 gaaattatca gcaagccatg aaaaaactcg tatcgattca tcatcaggat ttatcacccg
     2401 tactggcttt ccaggttgat ttttttccag tgcttgtttg acccagactg cttcatccaa
     2461 actattggta ttgatataga taacatgatc tctaccatat aaaatttcat aattcctgtt
     2521 tttgtttaca agaattttat cgatatgtct cagctgcgga tcattactgg cattaacaat
     2581 ataaaaagca aagaaaatga tagcaaaaag aaaaaaaagc attacatact taaacagctt
     2641 actgctaaag aaatgttgtt ttgtagaaat aatgttattt tcaataatgc ctggcaatga
     2701 atactgccaa acttcatttt tttcttttac agcaaatttg agacctaaaa cttctacttg
     2761 ctgattgaat gttaacgttc tatcttcagg ttcttgtgct gttaattctc tgattgttac
     2821 tactatatca gggtcggtag taattattat ctcaaaacta cctgtaagct cgccataagg
     2881 aacgactatt gtgttttctg gtaactcaga aaaagcatca cttcttccta acgaaacatc
     2941 attaccaatg ataacaagta gacgcccatt aagtatttca tactcgcatc cattcaatgg
     3001 tccgctcaac aaacgaatgg cataagattc caataagtct tgtgataaga atttatcatt
     3061 attttccatg aatacccccc gctaattcca taagctctag tttagagtag tgtaaca
//
LOCUS       DAZFZC010000239         3046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFZC010000239 DAZFZC010000000
VERSION     DAZFZC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.244"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..507
                     /locus_tag="ACHKXP_005171"
     CDS             <1..507
                     /locus_tag="ACHKXP_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649952.1"
                     /translation="KRKRDYYIICGSPDYINAIELDIINGLEYLVDAKKQLLIITSKK
                     INGRLTTYLFKTNQNMAQWLRCNMLMLNISVAKYIVKEFTSKQLNDLNELSQKLIEEL
                     KELPEREVKKGIRRSPEEVKSFILKIMEKNPGISATHALREFRDSGNSFEEKRFRTEF
                     MALREAKP"
     gene            581..1831
                     /gene="dpdA"
                     /locus_tag="ACHKXP_005172"
     CDS             581..1831
                     /gene="dpdA"
                     /locus_tag="ACHKXP_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase DpdA"
                     /protein_id="HFO7649953.1"
                     /translation="MSKLKYFFPDSQDFIDPSFDFFRETRNEHRVRQRDDQYPHEVFP
                     HPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPENMETMGDCGAFTYV
                     NQDVPPYRVEEVIEFYETSRFNHGVSLDHIVFGYEKPGEVFSGEVLAECRRRQDITLT
                     LAQEFLNKSQKSCFTPFGVAHGWSKTSYRQSVEALLAMGYRNITMGGMVPLKTAQILE
                     TLEEIKPLLKSDTRIHLLGIARPESFADFIKFGVTSIDSTTPLQQAFKDRKNNYHTPD
                     GPAYTAVRVPQFDANPSLSRKIKSGVIDQDIARHLEKDAMAALFEYDKGALSLEKTLD
                     AVLAYERLHSGEKEADKIRADYERTLGERPWNQCQCNICQAIGINVIIFRGAERNRRR
                     GFHNIQVLYNRLQHTLSLRSEELS"
     gene            1828..2961
                     /gene="dbpB"
                     /locus_tag="ACHKXP_005173"
     CDS             1828..2961
                     /gene="dbpB"
                     /locus_tag="ACHKXP_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein DpdB"
                     /protein_id="HFO7649954.1"
                     /translation="MSEYRVPALRIRQGEERQLYSLAIEGKQISKIAAISRIRRGEES
                     LVGYQRPEVRNHIREIQRYIESKNPMIPNPVIIAFDKRVRFEPLTDNGDMGHLVIPFS
                     EDKDFEKPGFIVDGQQRTAALRDAEIDSFMMPVSAFIANDAEEQREQFMLVNSTKPLP
                     KTLLYELAPHTHGRLPSDLQMRKFPSLLAQRLNFGEGPLAGRIKTATNPDGVIADNSM
                     IKMIDASLREGALYRFRDPATGLGDEGKMVKLLNNFWSAVETVFTDDWDKKPRYSRLL
                     HGVGILALGSLMDEIDQVHQDYKGEPGWTEIPSYTRFVEELNRIKPLCAWSGGVWNFG
                     TDIDGQPIVRKWNELQNLSKDISLVTDFLVMNYIKAVNADINT"
BASE COUNT          835 a          632 c          746 g          833 t
ORIGIN      
        1 aaaagaaaaa gagactatta tattatctgt ggttctccag attatattaa tgcaattgaa
       61 ctagatatca ttaatggtct tgagtatcta gttgatgcaa aaaaacaact gcttattata
      121 acttccaaaa aaatcaatgg taggttaact acataccttt tcaaaaccaa ccaaaatatg
      181 gctcagtggc tgagatgcaa tatgctcatg cttaatatca gtgtggcaaa atacattgtg
      241 aaggaattta cgtcaaaaca actcaatgat ttaaatgagt tatctcaaaa gttaatagaa
      301 gaactcaagg agttacctga gcgtgaagtg aaaaaaggaa ttcgtcgtag tccggaggaa
      361 gttaagtctt tcattttaaa gattatggag aaaaatcctg gtatcagtgc tacacacgcg
      421 ttgcgtgaat ttcgtgactc gggaaattca tttgaggaaa aacgttttcg gactgagttt
      481 atggccctga gagaggctaa accttaatgc tacttgcctg ggtttcatgg tagaatcccc
      541 cgttttttga ggctttgcag tggcgtcaca ggagagtaag ttgtcgaagc taaagtattt
      601 tttccctgat agtcaggatt ttatcgatcc cagtttcgac ttcttccgcg aaacgcgtaa
      661 cgaacatcgc gtgcgtcaac gtgacgatca gtatccgcat gaggtttttc ctcatcctta
      721 cgatggtatg ctggtttcta aagctgtagt tgatggtctt ggcggcggcg agagcaaata
      781 tactcgtgcc cagcgtttgc gttacttccg taatggaatg aagcactttt tccgtctgcc
      841 ggaaaatatg gaaactatgg gtgattgcgg agccttcaca tatgttaatc aggacgtccc
      901 gccataccgt gtggaagagg tgattgagtt ttacgaaact tcccgcttta accatggcgt
      961 ttctctcgat catatcgttt ttggctatga aaaacctggt gaggtattca gtggcgaagt
     1021 gttggctgaa tgtcgccgac gccaggatat cactctgacc ttggctcaag agtttttaaa
     1081 caagtctcaa aagtcctgtt tcactccctt tggcgttgca catggatgga gcaaaacctc
     1141 ctatcgtcag tctgtagagg ccctgctggc catgggatac agaaatatca ccatgggggg
     1201 gatggtgccg ttgaaaacgg cgcagatcct tgagactctc gaagagatta aaccgctgct
     1261 gaaaagtgat acacgtattc acttgcttgg cattgcccgt ccagagagtt tcgccgactt
     1321 tatcaagttt ggtgtgacca gtattgactc gacaacaccg ctgcagcaag ccttcaaaga
     1381 tcggaaaaac aactatcata cgcctgatgg cccggcctat accgctgtgc gtgtccctca
     1441 gtttgatgcg aatccaagtt tgagtcgcaa aataaaatct ggcgtgattg atcaggatat
     1501 tgcacgacat ctagaaaagg atgccatggc tgccctgttt gaatatgata aaggggcttt
     1561 gtcacttgag aagactcttg atgcagtcct ggcatatgaa cgcctccatt cgggtgaaaa
     1621 agaggccgat aaaatccgtg cggattatga acgtacgcta ggagaacgtc cttggaacca
     1681 atgccagtgc aatatttgcc aggccatcgg tatcaacgtc atcattttcc gcggagcaga
     1741 acgtaaccgt cgccgcggtt tccataatat tcaggtgttg tacaaccgtt tacagcacac
     1801 attgtcatta cgctcagagg aactgtcatg agtgaatatc gtgttcccgc cctgcgaatc
     1861 cgacagggtg aggagaggca actctatagc cttgcgatag aaggtaaaca gatcagcaaa
     1921 attgcagcga tttcgcgtat tcgtcgcgga gaagagagtt tggttggtta tcagcgtcca
     1981 gaagtacgta accatattcg cgaaattcag cgctatattg agagcaaaaa cccgatgata
     2041 ccaaacccgg tgattatcgc ttttgataag cgtgtgcgtt ttgaaccgct tacagataat
     2101 ggcgacatgg ggcatctggt gatacctttc tccgaagata aggattttga gaagccaggg
     2161 tttattgtgg atggtcaaca acgtactgct gctttgcggg atgcggaaat cgactcgttt
     2221 atgatgccgg tctctgcatt tatcgctaat gatgcagaag aacaacgcga gcagtttatg
     2281 ctggttaact ccacaaaacc tctgcccaaa acgttgttat acgaactggc accgcatacc
     2341 cacggtcgtt tgccatccga tctgcaaatg cgtaagtttc cttcgctgtt ggctcaacga
     2401 ctgaacttcg gggaaggtcc acttgcgggg cgcatcaaaa cggcaaccaa tcctgatggg
     2461 gttattgctg acaactcgat gatcaaaatg attgatgcta gtcttcgtga gggagcattg
     2521 tatcgcttcc gtgaccctgc gacagggttg ggggatgaag ggaagatggt gaaactgctg
     2581 aataacttct ggtcggcggt ggaaaccgtg tttaccgatg actgggacaa aaagccgcgc
     2641 tattcccgcc tattacacgg cgtcggtatt cttgcgctgg gtagcctgat ggatgaaatc
     2701 gatcaggtgc atcaggacta taaaggtgaa ccaggctgga ctgagatccc ttcctatact
     2761 cgttttgtcg aagagttaaa ccgcatcaaa ccgctttgtg catggagtgg tggcgtgtgg
     2821 aacttcggta cggatattga tggtcagcct atcgtccgta aatggaatga actgcagaac
     2881 ctgtccaagg atatatcatt agtaactgat tttttggtta tgaattatat caaagctgta
     2941 aacgcggata ttaataccta agtcaaaatg taagcccttt gtgtaaaaca aagggttttt
     3001 gtcatttcta ggccaataat ttcatattgg aactaaatgt tgggta
//
LOCUS       DAZFZC010000240         3007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFZC010000240 DAZFZC010000000
VERSION     DAZFZC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.246"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..220)
                     /locus_tag="ACHKXP_005174"
     CDS             complement(<1..220)
                     /locus_tag="ACHKXP_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7649955.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTL"
     gene            complement(230..1549)
                     /locus_tag="ACHKXP_005175"
     CDS             complement(230..1549)
                     /locus_tag="ACHKXP_005175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7649956.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(1530..>3007)
                     /locus_tag="ACHKXP_005176"
     CDS             complement(1530..>3007)
                     /locus_tag="ACHKXP_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7649957.1"
                     /translation="ESVDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFF
                     RLSHRPSWRYLGIGEEDARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAM
                     HAFNGELFVQATWDTSPSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAAL
                     GYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKM
                     LDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYY
                     AAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRN
                     YAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFS
                     FQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQ
                     QVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          570 a          953 c          841 g          643 t
ORIGIN      
        1 tcagtgtggt gctggtctgg tcagcagcaa ccgccagaat gccaacggca gcaccgtcgg
       61 tggtgccatc ccacgcaacc agcttacggg tggaggtgtc cagcatcagc ggggtcattg
      121 caggcgcttt cgcactcaat ccgccgggcg cggttgcggt atgagccggg tcactgttgc
      181 ccagcggctg gtaatgggta aaggtttctt tgctcgtcat aaacatccct tacactggtg
      241 tgttcagcaa atcgttaacg gcatcagatg ccgggttacc tgcagccagc ggtgccggtg
      301 ccccctgcat cagacgatcc agcgcagtgt cactgcgcgc ctgtgcactc tgtggtgctg
      361 cggccagaat gcggcgggcc gtttccacgg tcataccggg ggtttctgcc agcacgcggg
      421 cctgttcttc gcgtccgtga gcctcctcac agttgaggat ccccataatg cggctgtttt
      481 ctgccgcaac cgcagcggtg atctgcgcgt tcacgtccgg ctgcgccgcg ctggcgtttt
      541 cgccctccgt cgctggcacc acgtcagtaa cgtcagcctg cgaagcagtg gctgaaacag
      601 ttgttgattg agtctctttg gtcattcgcc ctcctgagag acgggattta cgtgcatcca
      661 gtgcatcacg cataacggtg atcgcatcgg tgctgttgac aagttcatca gccagtccgg
      721 catcaatggc ctcctgaccg ctgtacactg cagcctcggt atccagcaca gcctgcacgg
      781 acaggccggt atatgccgac accttctgcg caaacatccg gcgggttgca tccatccggg
      841 actgcagtgt ctcccggacg tcatccggaa gatggctgta ggggttgcca tccaccttat
      901 ggctgccgct gtaaatcagc gtgatttcca cgccctgttt ctccagcgca gcgccgtaat
      961 tactgtgagc catcatgacg ccgatggagc ctgtccgtgc ggtctgcgtg accagacgtc
     1021 gggaggcggc actggcaagc agctgacctg cactgcagtt catgtcgttg gccagcgccc
     1081 ataccggctt tatgtcacgc acacgggcga tgatgtcagc gcagtcaaat gctcccgcca
     1141 ccattccgcc cggtgtgtcc atatcgagca gaatgccgtc caccatcggg tcgctggcag
     1201 cctgttgcag acgggcgata atgccgttgt aaccggtcat ccccgagtac ggctgcagcg
     1261 cccgcgtccg gctgaccagc gtgccggaca ccggcagcac ggcgatgccg ttcatgacct
     1321 gataactgcg ggcctgtcgt ggtccgtcat catcaccgga taatgccagc gtcgcgggtg
     1381 cctcctgggc agtcaggctg tcgccggaca ccgcatccgt caggcggctg atcccaagct
     1441 ggcctgcaag cgcacaaaag aaaacccgcg cataggcggg ttcaagcatc agcggctcat
     1501 taaaggccat gctggcaata tgcgggagat tacgcagctc tgctgtcact cttctcctcc
     1561 tctgttgatt gtcgcagccc ggattcaaat gctgcagccg cccaggcggg cggtttaaga
     1621 ccagccgcgc ggcgctccat cgtttcacgg acctgctggg caaaaatttc ctgatagtcg
     1681 tcaccgcgtt tcgcgcactc tttctcgtag gtgctcagtc cggcttctat cagcatcacc
     1741 gcttcctgaa cttctttcag accatcgatg gccatacgac cggagcctat ccagtcgcag
     1801 ttcccccagg cactgcgggc ttcctgaaag ctgaagcgcg cttttgaagg taacgtcacc
     1861 acgcggcgaa cgatggcctc ttccagccag cacagaaaca tctggctcgc ctgacgggat
     1921 gcgacgaatt ttcgccgccc cataaagtac gcccacgact cgttcgcact ggcccgtgcc
     1981 gtggagtagc tcatctgggc gtaattccgg gaaagctgct catacgagac acccagcccg
     2041 gcagcgatat accgcagcag tgactgctca aacacggagt agccgttatc cgtgtcctga
     2101 gccgtctgca ggttcagtga gtcccccggc atcaggtgcg gcacttttgc gcctcccaga
     2161 cggaccggtg ctgcggcgta atacgcggca atttcaccaa tccagcccgt cagcctttcc
     2221 cgctgctcct gactgttcgc gcccagaata aaatccatcg ctgactgcgt atccagctca
     2281 ctttcaatgg tggcggcata catcgccttc acaatggcgc tctgcagctg cgtgttctgc
     2341 agcgtgtcga gcatcttcat ctgctccatt acgctgtaaa acacatttgc accgcgggtc
     2401 tgcccgtcct ccacgggttc aaaaacgtga atgaacgagg cgcgcccgcc gggtaactca
     2461 cggggtatcc atgtccattt ctgcggcatc cagccaggat acccgtcctc gctgacgtaa
     2521 tatcccagcg ccgcaccgct gtcattaatc tgcacaccgg cacggcagtt ccggctgtcg
     2581 ccggtattgt tcgggttgct gatgcgcttc gggctgacca tccggaactg tgtccggaaa
     2641 agccgcgacg gactggtatc ccaggtggcc tgaacgaaca gttcaccgtt aaaggcgtgc
     2701 atggccacac cttcccgaat catcatggta aacgtgcgtt ttcgctcaac gtcaatgcag
     2761 caacagtcat cttcggcaaa ctctttccat gccgcttcaa cctcgcggga aaaggcacgg
     2821 gcgtcttcct ccccgatgcc cagatagcgc cagcttgggc gatgactgag ccggaaaaaa
     2881 gacccgacga tatgatcctg atgcagctgg atggcgttgg cggcatagcc gttattgcgt
     2941 accagatcgt ctgcacgggc attgccacgg gtaaaattgg gcagcagggc tgcatccaca
     3001 ctttcac
//
LOCUS       DAZFZC010000241         2986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFZC010000241 DAZFZC010000000
VERSION     DAZFZC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.247"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..199)
                     /locus_tag="ACHKXP_005177"
     CDS             complement(<1..199)
                     /locus_tag="ACHKXP_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7649958.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVVWDGTTDGAAVGILAVAA"
     gene            complement(209..1528)
                     /locus_tag="ACHKXP_005178"
     CDS             complement(209..1528)
                     /locus_tag="ACHKXP_005178"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7649959.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASASSRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(1509..>2986)
                     /locus_tag="ACHKXP_005179"
     CDS             complement(1509..>2986)
                     /locus_tag="ACHKXP_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7649960.1"
                     /translation="ESVDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFF
                     RLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAM
                     HAFNGELFVQATWDTSSSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQLNDSGAAL
                     GYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKM
                     LDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSQEQRDKLTGWIGEIAAYY
                     AAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRN
                     YAQMSYSTARASANESWAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPPKARFS
                     FQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQ
                     QVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          585 a          947 c          818 g          636 t
ORIGIN      
        1 cagcagcaac cgccagaatg ccaacggcag caccgtcggt ggtgccatcc cacacaacca
       61 gcttacgggt ggaggtgtcc agcatcagcg gggtcattgc aggcgctttc gcactcaatc
      121 cgccgggcgc ggttgccgta tgtgccgggt cactgttgcc cagcggctgg taatgggtaa
      181 aggtttcttt gctcgtcata aacatccctt acactggtgt gttcagcaaa tcgttaacgg
      241 catcagatgc cgggttacct gcagccagcg gtgccggtgc cccctgcatc agacgatcca
      301 gcgcagtgtc actgcgcgcc tgtgcactct gtggtgcagc tgccagaatg cggcgggccg
      361 tttccacggt cataccgggg gtttcggcca gcacgcgtgc ctgttcttcg cgtccgtgag
      421 cctcctcaca gttgaggatc cccataatgc gactgttttc tgccgcaacc gctgcggtga
      481 tctgcgcgtt cacgtccggc tgcgccgcgc tggcgttctc gccctccgtc gcttgcacca
      541 cgccagtaac gtcagcctgc gaagcagtgg ctgaaacagt tgttgattga gtctctttgg
      601 tcattcgccc tcctgagaga cgggatttac gtgcatccag tgcatcacgc atgacggtga
      661 tcgcatcggt actgttaaca agttcatcag ccagtccggc atcaatggcc tcctgaccgc
      721 tgtacactgc agcctcggta tccagcacag cctgcacgga caggccggta tatgccgaca
      781 ccttctgtgc aaacatccgg cgggttgcat ccatccggga ctgcaatgtt tcccggacat
      841 catccggtag atggctgtag gggttgccat ccaccttatg gctaccgctg taaatcagcg
      901 tgatttccac gccctgtttc tccagcgcag cgccgtaatt actgtgagcc atcatgacgc
      961 cgatggagcc tgtccgggcg gtctgcgtga ccagacgccg ggaggaggca ctggcaagca
     1021 gctgacctgc gctgcagttc atgtcattgg ccagcgccca taccggcttt atgtcacgca
     1081 cacgggcgat gatgtcagcg cagtcaaatg cccctgccac catcccgcct ggcgtatcca
     1141 tatcgagcag aatgccgtcc accatcgggt cgctggcagc ctgttgcaga cgggcgataa
     1201 tgccgttgta accggtcatc cccgaatacg gctgcagcgc ccgcgtccgg ctgaccagcg
     1261 tgccggacac cggcagcacg gcgatgccgt tcatgacctg ataactgcgg gcctgtcgtg
     1321 gtccgtcatc atcaacggat aacgccagcg ccgcgggtgc ctctccggca gtcaggctgt
     1381 cgccggatac cgcatccgtc aggcggctga tcccaagctg gcctgcaagc gcacaaaaga
     1441 aaacccgcgc ataggcgggt tcaagcatca gcggctcatt aaaggccata ctggcaatat
     1501 gcgggagatt acgcagctct gctgtcactc ttctcctcct ctgttgattg tcgcagcccg
     1561 gattcaaatg ccgcagccgc ccaggcgggc ggtttaagac cggctgcacg gcgctccatc
     1621 gtttcacgga cctgctgggc aaaaatttcc tgatagtcgt caccgcgttt tgcgcactct
     1681 ttctcgtagg tactcagtcc ggcttctatc agcatcaccg cttcctgtac ttctttcaga
     1741 ccatcgatgg ccatacgacc ggagcctatc cagtcgcagt tcccccaggc acttcgggct
     1801 tcctgaaaac tgaagcgcgc ttttggaggt aacgtcacca cgcggcgaac gatggcctct
     1861 tccagccagc acagaaacat ctggctcgcc tgacgggatg cgacgaattt tcgccgcccc
     1921 ataaagtacg cccacgactc gttcgcactg gcccgtgccg tggagtagct catctgggcg
     1981 taattccggg aaagctgctc atacgagaca cccagcccgg cagcgatata ccgcagcagt
     2041 gactgctcaa aaacggagta gccattatcc gtgtcctgag ccgtctgcag gttcagtgag
     2101 tcacccggca tcaggtgagg cacttttgcg cctcccagcc ggaccggtgc tgcggcgtaa
     2161 tacgcggcaa tttcaccaat ccagccggtc agcttgtccc gctgctcctg actattcgcg
     2221 ccaagaataa aatccatcgc tgactgcgta tccagctcac tttcaatggt ggcggcatac
     2281 atcgccttca caatggcact ctgcagctgc gtgttctgca gcgtgtcgag catcttcatc
     2341 tgctccatca cgctgtaaaa cacatttgca ccgcgggtct gcccgtcctc cacgggttca
     2401 aagacgtgaa tgaacgaggc acgaccgccg ggtaactcac ggggtatcca tgtccatttc
     2461 tgcggcatcc agccaggata gccgtcctcg ctgacgtaat atcccagcgc cgcaccgctg
     2521 tcattaagct gcacaccggc acggcagttc cggctgtcgc cggtattgtt cgggttgctg
     2581 atgcgcttcg ggctgaccat ccggaactgt gtccggaaaa gccgcgacga actggtatcc
     2641 caggtggcct gaacgaacag ttcaccgtta aaggcgtgca tggccacacc ttcccgaatc
     2701 atcatggtaa acgtgcgttt tcgctcaacg tcaatgcagc agcagtcatc ctcggcaaac
     2761 tctttccatg ccgcttcaac ctcgcgggaa aaggcacggg cttcttcctc cccgatgccc
     2821 agatagcgcc agcttgggcg atgactgagc cggaaaaaag acccgacgat atgatcctga
     2881 tgcagctgga tggcgttggc ggcatagccg ttattgcgta ccagatcgtc tgcacgggca
     2941 ttgccacggg taaaattggg cagcagggct gcatccacac tttcac
//
LOCUS       DAZFZC010000242         2858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFZC010000242 DAZFZC010000000
VERSION     DAZFZC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.248"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHKXP_005180"
     CDS             complement(<1..194)
                     /locus_tag="ACHKXP_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7649961.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEK"
     gene            complement(178..453)
                     /locus_tag="ACHKXP_005181"
     CDS             complement(178..453)
                     /locus_tag="ACHKXP_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7649962.1"
                     /translation="MNLGEYLHHSGITQKHFAEIVGVTQGMVSHVITGRAKLTGKKIL
                     RWCEATGWIVTPHEIDGSTYPNPTDGLPVEYQANTQPEAGVIHENQA"
     gene            561..1061
                     /locus_tag="ACHKXP_005182"
     CDS             561..1061
                     /locus_tag="ACHKXP_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7649963.1"
                     /translation="MKRKSLSEIDLQAAQRLKEIWTAKKNQLGLTQERAAEILGFSTQ
                     GAVSHYLNGQTPLNLEAVIKFAGLLQVPPESIRPDMAELLQIVRMYPQESGEDNVVTI
                     SADMEQSENELPFNIDPMERDLLQTFRAFPKEDKEKMLKEMKEKKESIEEIVARWLAA
                     QKGRRA"
     gene            1079..1270
                     /locus_tag="ACHKXP_005183"
     CDS             1079..1270
                     /locus_tag="ACHKXP_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO7649964.1"
                     /translation="MSTALSPIISEFETVEQENSYNEWLRTKVAASLSDPRPAIPHDE
                     VMAEMENLIAQLAATNRSE"
     gene            1270..1560
                     /locus_tag="ACHKXP_005184"
     CDS             1270..1560
                     /locus_tag="ACHKXP_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7649965.1"
                     /translation="MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLT
                     EHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIEIVNVVHSRRQYPNKTC"
     gene            1830..1985
                     /locus_tag="ACHKXP_005185"
     CDS             1830..1985
                     /locus_tag="ACHKXP_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7649966.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            1987..2115
                     /gene="ydfB"
                     /locus_tag="ACHKXP_005186"
     CDS             1987..2115
                     /gene="ydfB"
                     /locus_tag="ACHKXP_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO7649967.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            2145..2363
                     /gene="ydfC"
                     /locus_tag="ACHKXP_005187"
     CDS             2145..2363
                     /gene="ydfC"
                     /locus_tag="ACHKXP_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO7649968.1"
                     /translation="MQKREPVIISPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(2665..>2858)
                     /locus_tag="ACHKXP_005188"
     CDS             complement(2665..>2858)
                     /locus_tag="ACHKXP_005188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649969.1"
                     /translation="TPDTENPPITLFLVLTVPRALSRIHRITKFTLKRAAEQSRSKTD
                     TAKRHQKIDNRGRCSGVVA"
BASE COUNT          812 a          656 c          678 g          712 t
ORIGIN      
        1 tctttctcta cccagcggaa aattttctgg gtattacggg ccagggcttc cggatggctg
       61 tcgtcgtaca gttccgggaa cgtcatcccc agttcgaaat aagtccgggc tatttcagct
      121 gctggaactt tctcaccgtc tggatacgcc caggcattca ttgccatgcg gatgtgttca
      181 tgcttgattt tcatgaatca cccccgcctc tggttgtgtg ttagcctgat actcgacagg
      241 taagccgtcg gttgggttgg gataagtact gccatcaatc tcgtgcggag ttactatcca
      301 gcctgttgct tcgcaccagc gtaaaatttt tttccccgta agtttcgccc gtccggtaat
      361 gacatggctt accatccctt gggttacccc aacaatttca gcaaaatgct tctgagttat
      421 accggaatga tgcaaatatt ctccaagatt cattgttcac ctcatgtgat gtcattacga
      481 tcattaatag cattgttatt tttaaaagta aatagcatta ctatttcaaa gagattaata
      541 atcttattag aattgaaggt atgaaaagaa aatccctgtc agagatcgac ctgcaagccg
      601 cccagagact gaaagaaatc tggacggcga aaaaaaatca actagggtta acccaagagc
      661 gtgcggcaga aattctggga ttttcgacac agggagctgt aagccattat ctaaatggtc
      721 agacaccttt aaatcttgag gctgttatca agttcgcagg gttgctgcaa gttcctcccg
      781 agtcaatcag accagatatg gccgagttgt tacaaattgt aaggatgtat ccccaagaat
      841 ctggggagga caatgttgtc actatatctg cagatatgga acaatcggaa aacgaacttc
      901 cgtttaatat agaccccatg gagcgggatt tgctccagac gttcagggct ttccccaaag
      961 aagataaaga gaaaatgctt aaggaaatga aggagaaaaa agaatcaatt gaagaaatcg
     1021 ttgcgcgatg gctagctgcg caaaagggtc gtcgcgccta atctgaggag gtaaaaacat
     1081 gagtacagcc ctttccccga taatttctga atttgaaaca gtcgaacaag aaaacagcta
     1141 taacgaatgg ttgcgaacca aagtggcagc aagcctctca gatccccgtc ctgcaattcc
     1201 acatgacgaa gtaatggctg aaatggaaaa ccttattgct caattagctg caacgaacag
     1261 gagtgagtaa tgctgcccat tttatggcta ccttctgcac gtgatgattt acgtcagatc
     1321 gtagcctata ttgctaagga aaaccctccc gctgcacgta gactaaaaat acgcattgaa
     1381 acatcagttt tgtcacttac tgaacaccct tatctgtacc caccgagcga aagagttcca
     1441 agtcttcgtg agatagtgac tcatcctaac tacataatac tttaccgagt aacagcatct
     1501 aacatcgaga tcgtaaatgt agttcactca cgaagacagt atccaaacaa aacctgttaa
     1561 tccttcctgt caacaaccac cttcgggtgg tttttttctt gccacgataa tagcactgct
     1621 atttacataa ttaaatagta gtggtattgt tcattcatca acccaccccg ccccacagaa
     1681 cgccaggcaa tacttcgagt tacccggcag tggtcagggg ttaagtagcc agcccgaggc
     1741 gtatgaacat gacggcggga acactttata taacagcgca gcagtttttt agttccgcta
     1801 ccccggcgtt aaggggaaat gaggtcagca tggatactat cgatcttggc aacaatgaat
     1861 ctctggtgta cggcgtgttt cccaaccagg acggcacatt caccgcgatg acgtatacca
     1921 gaagcaaaac gtttaaaacc gaaaatggtg cccgtcgctg gctggaaaga aactcaggtg
     1981 agtgatatgg atttcgacac aatcatggaa aaggcttacg aagaatactt cgaaggcctt
     2041 gccgaaggcg aagaagctct cagcttcagt gagtttaaac aggcgctttc cagctcggca
     2101 aaatctaacg gctgataagc gaagcagcac cgcgaggaat cagtatgcag aaacgagaac
     2161 ccgtcatcat ctcgccagac tataccgatg atgaacttta tgagtggatg cgccagaaaa
     2221 ttaatgcagc gcaggatctg aaatgggcca atgaagccag ggctaagcag gctgaaaatc
     2281 tgtccgctct ggagcaggat atcaccaggc tggaaaaagc agcggcatta agcattgcca
     2341 gaatgattac atacccgcgt taatagctaa ccaacgaagc taaggttggt aattaaggag
     2401 ttctccacgg gtgaggtgga gtgcgtgcgc cggacatggg tgagcatccg gcactgacag
     2461 tttactgaaa ggatatttcc atgaaaagtc agaccataac gcgaaagcgc acggcgaggt
     2521 agctggttca tagatagcct gtcgttaaat tttcgtcgac cgtgcgcttc cggttgtggc
     2581 actccgcgaa atggcgcggc ggtaagtatg gcggggttat tccttccccg ttgaggacac
     2641 cgggttgtca ggttgaccat acgcttaagc gacaaccccg ctgcaacgcc ctctgttatc
     2701 aattttctgg tgacgtttgg cggtatcagt tttactccgt gactgctctg ccgccctttt
     2761 taaagtgaat tttgtgatgc ggtgaatgcg gctaagcgca cgcggaacag ttaaaaccaa
     2821 aaacagtgtt atgggtggat tctctgtatc cggcgtta
//
LOCUS       DAZFZC010000243         2827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFZC010000243 DAZFZC010000000
VERSION     DAZFZC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.249"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1888
                     /locus_tag="ACHKXP_005189"
     CDS             <1..1888
                     /locus_tag="ACHKXP_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7649970.1"
                     /translation="KELTDKDRGRRDENYNIIKDLVDDRMFLFDYALHKKSHLLMDYS
                     RNKKISQYTIRTLLALYWRHGQDIYALLPAFSNCGAAGKSRIKHEIKLGNSKKNRALP
                     NERSRVFILNERDINNIRKSLITYHYKVNGDTIKKTLERHIDLYFRDEIKTANLENRA
                     PYVPSLKQFSYWNKKLFTKDFSINKKNTKKEIDLKMRALLGSVANTTVLPGDVFEIDS
                     TVADVHLISSLNRRKVIGRPTIYTVVDRATRMIVGLHVSLYHASWRAARQALANCFMP
                     KKEYCRLFGISITDDDWPCSHIPLTLMCDNGEMIGLKPQEEMTPLTKLEFAPVGRGDR
                     KSIVERCFGILNDEVIHRLIGTTRRGKIVKGEPTPQSRACLTIQEVTSLLIREILAHN
                     QRTYEELAYINPLLIENDLVISPKNSWMISLKHGRFSARAVGADEVIARLLIPVNANI
                     TAGGIQYNNLFYECDPEIASGVRVFGRTTCEARIDDNCVDYIYVRFDKNSIFKKHYLL
                     KKRDVFKGKAHLDTDVMADWVDTQKEINLFTLDSLSNINNKDEFNRKGNERLNEIYES
                     RRVHGKDIKKNRKNELDSLGRTHVANGRSEPLLPSSSNVLLLPGREEKQRWLKGKKKT
                     EDAE"
     gene            1888..>2827
                     /locus_tag="ACHKXP_005190"
     CDS             1888..>2827
                     /locus_tag="ACHKXP_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7649971.1"
                     /translation="MNLIRRVSAIYKEQELPEYRGNPLIEALPEALTEDEVLLEMSYF
                     PEIDEKIRWTAPANVREQYVERIKKFRCPQTNLIQAYKMILRALRESYAARNPLKSGT
                     IQYLHYYGNERPDIEPESGYFKSQAETITIVGMSGSGKTTMIEQVMDHFPQIIEHSSY
                     KGVFPGFSKQIVWVKINCPYNSSVRDLCEEILQKLDDAIGIERTTPEIRNGALARQIA
                     QRIKSSFLGILVIDEMQRLKFSRTGGESKLIDFLHEIVDSMGVSMVFCGNHPFDETLT
                     KKMRIARRAESGGYMKIKNVRYDSQDWQSFIHYLWPL"
BASE COUNT          968 a          464 c          605 g          790 t
ORIGIN      
        1 gaaagaatta acagataaag acagaggaag gcgagatgaa aattacaata tcattaaaga
       61 tcttgtagat gacagaatgt ttctgttcga ctatgcatta cataaaaagt cgcacctttt
      121 aatggattac tcaagaaata aaaaaatatc acaatatact atcagaacgc ttctggcgtt
      181 gtattggcga catgggcagg atatttatgc attgctaccc gcattctcga actgtggcgc
      241 cgctgggaaa agtagaatca aacatgaaat taaacttggg aattccaaaa aaaacagagc
      301 attacctaat gaacgatcac gtgttttcat tcttaatgaa agagatataa ataatattag
      361 aaaatctctc attacgtatc attataaagt taacggagat acgataaaga aaacattaga
      421 gagacatatt gatttgtatt ttagggatga aattaaaaca gcaaacctag aaaatagagc
      481 tccatatgtt ccttctttaa aacaattttc atactggaat aaaaaactct tcactaaaga
      541 tttctcgata aataagaaga acacgaaaaa agaaatagac ctcaaaatgc gggcgctttt
      601 aggtagtgtg gctaatacaa ctgttttgcc gggtgatgtt ttcgaaatag actcaactgt
      661 cgctgatgta catcttattt cgagtttaaa cagacgaaaa gtcattgggc gtcctactat
      721 ttatacagtg gtggatcgtg caacaagaat gattgttggc cttcatgttt ctttatacca
      781 tgcttcatgg cgagccgccc gacaagcgtt agcaaattgc tttatgccaa aaaaagaata
      841 ttgtagatta tttggaatct ctattactga tgatgattgg ccttgttctc acattccatt
      901 aacattgatg tgtgacaacg gggagatgat tggtcttaaa cctcaggaag agatgacccc
      961 cctgacaaaa cttgagtttg cgccagtcgg tagaggcgac agaaaaagca ttgttgaacg
     1021 ctgtttcggc attctgaatg atgaggttat tcataggctt attggaacaa cacgaagggg
     1081 aaagattgtt aaaggagagc caacaccgca atccagggct tgtttaacga ttcaggaggt
     1141 cacgtcttta ctgattcggg agatacttgc acataatcag agaacgtatg aggaactggc
     1201 ttatatcaat cccttactga ttgaaaatga tctcgtaata tcaccaaaaa atagttggat
     1261 gataagctta aagcacggga gattcagcgc aagagccgtt ggggccgacg aagtgatcgc
     1321 acgattgtta atccctgtga acgctaacat taccgccggt gggattcagt acaataatct
     1381 tttttatgaa tgtgatcctg aaattgcatc aggtgtcaga gtatttggaa gaaccacttg
     1441 tgaagcaaga atagatgaca attgcgtaga ttatatatat gtaagatttg acaaaaatag
     1501 cattttcaaa aaacattacc tacttaagaa aagagatgta tttaaaggga aagctcatct
     1561 tgatacagat gtaatggctg attgggtaga tactcaaaaa gaaattaatt tatttactct
     1621 ggattcgctc agcaatatca ataataaaga tgaatttaac aggaaaggta acgaaagatt
     1681 aaatgaaata tacgaatctc gccgggtaca tggtaaagat attaaaaaga acaggaaaaa
     1741 tgaacttgat tcattagggc gaactcatgt tgctaatgga agatctgaac ccctacttcc
     1801 ttcatccagt aatgttctgc ttttgcctgg ccgggaggag aaacaaagat ggttaaaagg
     1861 taagaagaag actgaggacg cagaataatg aatcttatac ggcgcgtttc agcgatatat
     1921 aaagaacagg agttacctga gtatcgtggc aatcccttaa ttgaagcact tcctgaagcg
     1981 cttacggaag atgaagtact tctggaaatg agttatttcc ccgaaattga cgaaaaaatc
     2041 cgctggactg ccccggcgaa tgttagagag cagtatgtcg aacgtataaa gaaattccgt
     2101 tgccctcaaa ccaatcttat ccaggcttat aagatgatct tacgggcact tagggaaagc
     2161 tatgcagctc gtaatccttt aaaaagcgga acaattcaat atcttcatta ctatggaaat
     2221 gagcgtcctg atattgagcc agaaagtgga tattttaaat cccaggctga aactattacg
     2281 atagttggaa tgagtggttc tggaaaaact accatgattg agcaagtcat ggatcatttc
     2341 cctcagatta tagaacacag cagctataaa ggagtttttc ccggcttcag taagcaaatt
     2401 gtatgggtaa agattaattg tccatacaat tcaagtgtga gagacctttg tgaagagatt
     2461 ttacaaaaat tagatgatgc aattggtatt gaacgaacta cacctgagat tagaaatggt
     2521 gctctggctc gccagattgc acaaagaatt aaatcatcat ttttgggtat ccttgttatt
     2581 gatgaaatgc agaggcttaa attttcaaga accgggggtg agagtaagtt gattgatttt
     2641 ttacatgaaa ttgtagattc catgggggta tctatggttt tttgtggaaa tcatccattt
     2701 gacgagacgc tgacaaagaa aatgagaatt gccaggcggg cagagtctgg tggttacatg
     2761 aaaattaaga atgttagata cgattcacaa gactggcaat cgtttattca ttatttatgg
     2821 ccattac
//
LOCUS       DAZFZC010000244         2793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFZC010000244 DAZFZC010000000
VERSION     DAZFZC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.250"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..203
                     /locus_tag="ACHKXP_005191"
                     /pseudo
     CDS             63..203
                     /locus_tag="ACHKXP_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(285..372)
                     /locus_tag="ACHKXP_005192"
     tRNA            complement(285..372)
                     /locus_tag="ACHKXP_005192"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(336..338),aa:Ser,seq:tga)
     gene            799..1917
                     /gene="hyaA"
                     /locus_tag="ACHKXP_005193"
     CDS             799..1917
                     /gene="hyaA"
                     /locus_tag="ACHKXP_005193"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO7649972.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1914..>2793
                     /locus_tag="ACHKXP_005194"
     CDS             1914..>2793
                     /locus_tag="ACHKXP_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HFO7649973.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIG"
BASE COUNT          682 a          756 c          718 g          637 t
ORIGIN      
        1 tgccaaattt gctattaacc ttttgaatat aaagcactaa ttatctattt ctgttgcctt
       61 cttcagtaga tctggagatc gcaacggagg aagaaaactc gttgctggaa gcctggaaga
      121 agtatcgggt gttgctgaac cgtgttgata catcaactgc acctgatatt gagtggccgg
      181 aagaaccaag atcatccgaa taaaaatata ccaattggta taccaagttt ataccaagag
      241 ccagaaaagc aaaggggcta ccatttggta accccttgat tatttggcgg aagcgcagag
      301 attcgaactc tggaaccctt tcgggtcgcc ggttttcaag accggtgcct tcaaccgctc
      361 ggccacactt ccggaatgac gcgcactata aacatcccga tgcggcgtgt aaacccctaa
      421 tttgtttgtt tgcctgaaaa acagccaaaa gtgcattgat agcgtgaaat aacagcagat
      481 tgatcatttc atcaccatga attccttctc ttttactcgt ttagcaaccg gctaaacatc
      541 cccaccgccc ggccaaaaga aaaataggtc catttttatc gctaaaagat aaatccacac
      601 agtttgtatt gttttgtgca aaagtttcac tacgctttat taacaatact ttctggcgac
      661 gtgcgccagt gcagaaggat gagctttcgt tttcagcatc tcacgtgaag cgatggtttg
      721 ccttgctaca gggacgtcgc ttgccgacca taagcgcccg gtgtcctgcc ggtgtcgcaa
      781 ggaggagaga cgtgcgatat gaataacgag gaaacatttt accaggccat gcggcgtcag
      841 ggcgttaccc ggcgcagctt tctcaaatat tgtagtctgg ctgccacgtc gctgggatta
      901 ggcgcgggaa tggcaccaaa gattgcctgg gcgctggaga acaaaccgcg cattccggtg
      961 gtatggatcc acggtctgga atgcacctgc tgtaccgaat cttttatccg ctccgctcac
     1021 ccactggcga aggacgtcat cctttccctg atttccctcg attacgacga tactttgatg
     1081 gctgccgccg gaacccaggc ggaagaagtc tttgaagaca tcatcacgca atacaatggc
     1141 aaatatatcc tcgcagtaga aggtaatccg ccgctgggcg agcaggggat gttctgtatc
     1201 agcagcggtc gaccgtttat tgagaaactc aaacgtgccg ctgccggagc cagcgcgatt
     1261 atcgcctggg gaacctgcgc gtcctggggc tgcgtgcagg ccgcgcgacc caatccgacg
     1321 caggcaacgc ctatcgacaa agtcatcacc gacaaaccca ttatcaaagt acctggctgc
     1381 ccgccgatcc cggatgtgat gagcgccatc attacttaca tggtgacctt tgatcgcttg
     1441 ccagatgtcg acagaatggg ccgtccgctg atgttctatg gtcagcgaat ccacgataaa
     1501 tgctatcgcc gcgcccactt cgacgccgga gagttcgtcc agagttggga tgatgacgct
     1561 gcccgcaaag gttactgcct gtacaaaatg ggctgcaaag ggcctaccac ctataacgcc
     1621 tgttcctcca cacgctggaa tgatggcgtt tctttcccaa tccagtctgg tcacggctgc
     1681 ctgggctgtg cggaaaatgg tttctgggat cgcggttcgt tctacagccg cgtggtcgat
     1741 attccgcaaa tgggtactca ttccaccgcc gataccgtcg gtttaaccgc gcttggcgtg
     1801 gtggcagcgg ctgttggtgt gcacgcagtc gccagcgccg ttgaccagcg cagacgtcat
     1861 aaccagcaac ctacagaaac cgaacatcag ccaggcaatg aggataaaca ggcatgagca
     1921 ctcagtacga aactcaggga tacaccatca ataatgccgg acgccgcctg gtggtcgacc
     1981 cgattacgcg catcgaaggc cacatgcgct gcgaagtgaa tattaacgat cagaatgtga
     2041 tcaccaatgc cgtctcctgc ggcaccatgt ttcgcgggct ggagatcatc ctacaagggc
     2101 gcgacccgcg cgatgcgtgg gcgttcgttg aacgtatctg cggcgtctgt actggcgtac
     2161 acgccctggc ttcggtttac gccatcgaag atgctatcgg tattaaagtg ccggacaacg
     2221 ccaatatcat ccgcaacatt atgctggcaa cgctctggtg ccacgatcat ctggtgcact
     2281 tctatcagct tgccgggatg gactggatcg atgtgttaga tgcgctgaaa gccgacccgc
     2341 ggaaaacctc cgaactggcg caaagtctct cctcttggcc gaaatcatcc cctggctatt
     2401 tcttcgacgt acaaaaccgc ctgaaaaaat ttgttgaagg cgggcagttg gggatcttcc
     2461 gcaatggcta ctgggggcac ccgcagtaca aactgccgcc agaagctaac ctgatgggct
     2521 ttgcccacta tctcgaagct ctcgatttcc agcgtgaaat tgtcaaaatc cacgcggtct
     2581 ttggcggtaa aaacccgcat ccaaactgga ttgtcggcgg gatgccttgc gccatcaaca
     2641 ttgacgaaag cggcgcggtc ggggcagtca atatggaacg cctgaacctg gtgcagtcaa
     2701 ttatcacccg cacggcggac ttcattaaca acgtgatgat ccccgacgcc ttagccatcg
     2761 gtcagttcaa caaaccgtgg agcgaaatcg gca
//
LOCUS       DAZFZC010000245         2779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFZC010000245 DAZFZC010000000
VERSION     DAZFZC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.251"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..308)
                     /locus_tag="ACHKXP_005195"
     CDS             complement(<1..308)
                     /locus_tag="ACHKXP_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7649974.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     "
     gene            complement(836..1021)
                     /locus_tag="ACHKXP_005196"
     CDS             complement(836..1021)
                     /locus_tag="ACHKXP_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7649975.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
     gene            complement(1249..1398)
                     /locus_tag="ACHKXP_005197"
     CDS             complement(1249..1398)
                     /locus_tag="ACHKXP_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649976.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            complement(1398..1967)
                     /locus_tag="ACHKXP_005198"
     CDS             complement(1398..1967)
                     /locus_tag="ACHKXP_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO7649977.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            complement(2242..2775)
                     /locus_tag="ACHKXP_005199"
     CDS             complement(2242..2775)
                     /locus_tag="ACHKXP_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7649978.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          704 a          759 c          554 g          762 t
ORIGIN      
        1 ttttttgacc gcgcgtttcc ggacgtattc tgttctcctg tccctttata tcgtcggaat
       61 acccgccgct cttcaaatcc cattcccaac tcagaatgta gtctgttgac cgcttgtttt
      121 atttcggtca ggttcaccgg tgaaaccgga gtccggcgcg ccttacgcaa acactctgct
      181 cgtttctgtg ccgccacttt tcttttctgg tcatcactta gctgtaccat cacttttgcc
      241 catcgttcag ctgctctccg gtacagtcct tttttctcca gacattctgc cacgtgatca
      301 tgtagcataa gtgacctccg attatctaca gactgccatc ctgaatttac cttcccttaa
      361 tgaaataaca ataaaaaaca agccacgcaa aaacaataaa acaacacaca aaaaaaacta
      421 aataataaac aaaaataatc accttatttt attatttttt gagggagcaa ttactgaaca
      481 aaaaacgctg actatatact caaaaccaaa caactattct gccaatcagg tatcatggca
      541 acacacggaa ttaccgtgtt tttgccttct ctgcccatac aatacgggca tatacttcat
      601 tctctattgt aatatttcta tccatgtgcc ccactccatt tacctgtaaa taatattcaa
      661 aatatttatc acagaaatcg tttttggcca tgaactgagc acactataaa gtccggaact
      721 gactctttgt taaattacct taacgttacc agtaacacct tcgtaacaaa acatcacggt
      781 atacactggg tacggatata ttcctgtgct ccttccagtt gcttctgcat tgccatcagc
      841 cgttctctga ggatgaaata atcccgttca gcggcttctg ccagtcgggg accggttgca
      901 ttatccacgc cggaggtgat gggggcttta cgcaaggagc ctggacagtt ggcgttgatg
      961 cgcaggcgct tacgaccagc ggcaacatca gcacgcagag tttcattttc agctctcgca
     1021 tcggctaatt ccctcgagta tctggcatca agtgcagcaa catcacgctg gcgctgctgc
     1081 atatcagtaa tggttgcatt tgccagctcc agctcactga cttttttatc gcgctgctct
     1141 ttgtaggtta tggcgttatc gcggtaatga ttcagcccca gactaagtgc accacaggct
     1201 accagcagga caataatcac cacacacaga acacggttca tctctctttc accccaccag
     1261 tcccgataac gtcaggactc gccaagcggt ggaaaagaaa atggcaacca gaatgactaa
     1321 aaatgaaatg ccgacgatta cacagaggat cttcgccagc gttatgagtt tatccgatat
     1381 cattagccac cactccatca atccgccttt gttattttcc ctttgtctgt atcagccagg
     1441 acaaaatcaa tcagcagatt cgcttcgttt atcaatgtgc ggatttttga tacatgcgcg
     1501 gatttaacct gtttccactc attcagcccg gtagcaaaca cactggcaat gtttttatcc
     1561 cgtttcatgt cagcgcaagc ctgattgagt tcttccatca cactcattcg acggggatta
     1621 acgacaaaac ccttcgtcca gtattcatag agaacatcgt cacactcttc ctgatactgg
     1681 attaccttgc cgcggatttc gggttttact ttgttgggat tgatggtttg taaccagcct
     1741 gcaagttttc gaagcggcag ggacaccata ttgcgtcgtt tcccatcctc agcaaccata
     1801 acgatttccg ttatagttga cgcaaaacgc tgtcttaact tagccaactg tgattgccag
     1861 gccagcccca tccccgcaac gacaggtttc atgggaacgt atggttcgcc gttatggtta
     1921 accacataaa gagaatcgcc gtgaaacggc acggtcatca tattcatcgg ttatttcctt
     1981 ttagtgatga accctgcgca caggaataac cagcccaaag agggttaacc agaccactgc
     2041 cggttatcca ccagggctca tcctgaaagg ttctttggtt tatttacgct tgtgcgaagc
     2101 gcagaaatga caaaggcacc attacggtgc ctcttcatga aacaatcttg ttgactttat
     2161 tcacttacat tttgccagtt cgcaggattt cgtgttgtct acccgcgttg gccaacgtca
     2221 tttttcagca aaatattctg cttacctgtc gataccccag catgccagcg cactctcctg
     2281 gtcacgacgg gatacctgac cgtaacagtt gtttgaacgg atacggcagt ctctgccacc
     2341 gtccttaatc caccagcgaa tcgcctcaca cgctcccctg cgatcgcctg cattaagccg
     2401 cttataaaac gtcgacggga aacacttacc ggggccaatg ttataggggc aaaatgacgc
     2461 gatacctgct ttttgtggct cggtcagtgg aacttttata ttacgctcca cccatgccag
     2521 cgccttatcc cgttcgatag cgttaacccg gtcgcatttt tccttcgaca gcttcatgcc
     2581 aggaatcaca ggcttaccat ccaccatgat ggcgcctcgg cagatggtcc agatacccgc
     2641 accatcacgg tatgccgtgg tgtggttgcc ttccttttca tccagaaact ggtcgaggat
     2701 ttcaggcgca gaagcacctg cggcaatcag cgccagaacg gcagccgata aaccatagcg
     2761 gagtttcctg ctcatcagc
//
LOCUS       DAZFZC010000246         2740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFZC010000246 DAZFZC010000000
VERSION     DAZFZC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.252"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1692
                     /locus_tag="ACHKXP_005200"
     CDS             <1..1692
                     /locus_tag="ACHKXP_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7649979.1"
                     /translation="EAIVDNGASFEPLSGSLNSVIPPAVQHLTVEVSAADGQYLAQAK
                     WDTPRVVKGVRFSLRLTNGSGEDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINRYG
                     QQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSEAKIADTS
                     QVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEVSGQPSNDGEGYL
                     EFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSKTLEDQSATI
                     QQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIA
                     MINPESGNSTPLFVAQGNQLFMNDVFLKRLFAASITSSGNPPTFSLTPEGKLTARNAD
                     ISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRNNSYASGTVTVT
                     VYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHEPATDV
                     SRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKKSTAGISIS"
     gene            1761..2384
                     /locus_tag="ACHKXP_005201"
     CDS             1761..2384
                     /locus_tag="ACHKXP_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7649980.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAENGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            2449..>2740
                     /locus_tag="ACHKXP_005202"
     CDS             2449..>2740
                     /locus_tag="ACHKXP_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7649981.1"
                     /translation="MAVKISGVLKDGTGKPVENCTIQLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGVPPSHAGTITVYEDSQPGTLNDFLGAMTEED"
BASE COUNT          660 a          666 c          832 g          582 t
ORIGIN      
        1 gaagccattg tggataacgg ggccagcttt gagccactgt ccggttcgct gaacagcgtc
       61 atcccgccgg ctgtgcagca cctgacggtg gaggtgagcg cggctgacgg tcagtatctg
      121 gcacaggcga aatgggacac gccgcgggtg gtgaagggtg tgcgcttcag tctgcgcctg
      181 accaacggaa gcggagaaga cagccgtctg gtgaccaccg ccatcactgc ggatacagag
      241 catcgtttca gtggtctgcc gctcggggaa tacaccctga cagtcagggc aattaacagg
      301 tatggccagc agggcgaacc ggccaccacc accttccgga ttaacgcgcc agcaaaaccc
      361 gccaccattg aactgacgcc ggggtatttt cagataacgg cggtcccgcg tcttgcggtg
      421 tatgacccga cggtacagtt tgagttctgg ttttcggagg caaaaatcgc agacacatct
      481 caggtggaaa cctctgcccg ttatctgggg accggcagtc agtggagtgt atccggcccg
      541 cacattaagc ccgggaagga tttctggttt tacgtgcgca gcgtcaacct ggtggggaaa
      601 tctgcgtttg tggaagtcag cgggcagccc agcaatgatg gtgaagggta tctggaattt
      661 ttccgggaaa aaataggaaa actgcatctg gctcaggggc tatgggagct gatagacaac
      721 agccagcttg cggatgagat ggcggagatg aagaccacca tcaccgaaac ccgcaatgaa
      781 atcacacaga cggtcagtaa aacgctggaa gaccagagcg ccaccattca gcagatacag
      841 cgcgtgcaga aggacacaaa tgatgacctg gctgcgctgt acatgctgaa ggttcaaaaa
      901 acgaaagacg gcattcccta tgtggccggg attggtgcag ggattgagga tactgatggc
      961 cagccactga gcaacatact gctgctggct gaccgtatcg cgatgataaa tccggagagc
     1021 ggcaacagca ctccgttatt tgtggcgcag gggaatcagc tgttcatgaa cgacgtgttc
     1081 ctgaagcgtc tgtttgcggc gagtatcacg tcatccggca accccccgac gttttccctg
     1141 acgccggaag ggaagctgac agccaggaac gcggatatca gcggagcaat taccgcgaat
     1201 accggcacgc tcaataatgt caccattaac gagaactgtg tcatcagagg gaaactgtct
     1261 gcaaaccaga ttgaaggcga cctggtgaag acggtgggga aagcctttcc ccggaataac
     1321 agttatgcca gcgggacggt aaccgtcaca gtttacgatg accagggctt cgaccggcag
     1381 attatcattc ccccggtgct gtttcgcggg acgaaacacc agaatttcaa cagcccgaat
     1441 cagcagtcgt actggtattc cacctgtaag ctgcaggtgc tgaagaacgg ggttgagatt
     1501 ttccatgaac cggcaacgga tgtcagccgg gtgttctcat cggtgataga tatgccggca
     1561 gggcggggtc atgtcaccct gacgtttaat gtgtcgtcgg ccggtgcgaa caactggacg
     1621 ccgacaacgt acatcagtga tttactggtt gtggtcatga aaaaatccac ggcagggatc
     1681 agtatcagct gacggtttat taacccggac gggcacctca ggaggtgcct tttttattga
     1741 ctgaaaacaa agaggtaatc atgcggcatt tatacgcaac gatattattg tttactaccc
     1801 tgctggcagg aattgccttt cctgcacagg ctgaaaacgg acacggtgca ttttccgtgg
     1861 gatatgctca ggttcacccg ggcggcgtac cggcattgtc cggtaccggt gcgcgtgcag
     1921 gtgatttaaa agggattaat gtgaaatacc gttatgagtt cacggatcac ctgggcggca
     1981 ttgtcgcgct gagttatgca tcggtgaaga aaagtgacac gatgaagacg ggtgaaaata
     2041 ccttccatta tgaaagcctg cgcggtcgtt atgtcagtct gatggccggc cctgtctggc
     2101 agctcagtga gcgggtcagt ctctatggca tggccgggat ggcgtacacc cgctggtctg
     2161 acagtgttca ggattaccgg cgtgatgaag tgaaaccggg gtatgtgaag gagaccacca
     2221 ccgccagtga tggtcatact gcgcgtcatc tgtcgccggc ctggaatgcc gggattcagt
     2281 tcagtcccgt agagacggtg gttattgacc ttgcttatga aggttccggc agtggcgact
     2341 ggcgcactga cggtttcatc gtgggtgtcg gctataaatt ctgattagcc aggtaacaca
     2401 gtgttatgac agcccgccgg ttcaggcggg cttttttgtg gggtgaatat ggcagtaaag
     2461 atttcaggtg tactgaaaga cggcacagga aaaccggtag agaactgtac cattcaactg
     2521 aaagccagac ggaccagcag cacggtggtg gtgaacacgg tggcctcaga aaatccggat
     2581 gaagccgggc gttacagcat ggacgttgag tacggtcagt acagcgttat tctgttggtg
     2641 gaaggggtcc cgccgtcaca tgccgggacc atcaccgtgt atgaagattc tcagccgggt
     2701 acgctgaatg attttctcgg tgccatgacg gaggaggatg
//
LOCUS       DAZFZC010000247         2710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFZC010000247 DAZFZC010000000
VERSION     DAZFZC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.253"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..93)
                     /locus_tag="ACHKXP_005203"
     CDS             complement(<1..93)
                     /locus_tag="ACHKXP_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649982.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKL"
     gene            complement(244..714)
                     /locus_tag="ACHKXP_005204"
     CDS             complement(244..714)
                     /locus_tag="ACHKXP_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7649983.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPVPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(723..1045)
                     /locus_tag="ACHKXP_005205"
                     /pseudo
     CDS             complement(723..1045)
                     /locus_tag="ACHKXP_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1359..2009)
                     /locus_tag="ACHKXP_005206"
     CDS             complement(1359..2009)
                     /locus_tag="ACHKXP_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7649984.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHKRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRRFLKPLNPQYPMIEINGNCKIIGVVVDAKIANLP"
     gene            2090..2275
                     /locus_tag="ACHKXP_005207"
     CDS             2090..2275
                     /locus_tag="ACHKXP_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7649985.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEV
                     VTAGALKYQESAYRKAA"
     gene            2384..2662
                     /locus_tag="ACHKXP_005208"
     CDS             2384..2662
                     /locus_tag="ACHKXP_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO7649986.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAKLAKKVAMVLTKEKPQDCCNSFEA"
BASE COUNT          715 a          611 c          558 g          826 t
ORIGIN      
        1 aagtttttcc tctgtcatta cgtcatggtc gatttcaatt tctattgatg ctttccagtc
       61 gtaatcaatg atgtattttt tgatgtttga catctattca tatcctcata gataaaaaat
      121 cgccctcaca ttagagggca aagaagattt ccaataatca gaacaagtcg gctcctgttt
      181 agttacgagc gacattgctc cgtgtattca ctcgttggaa tgaatacaca gtgcttattc
      241 gtactaataa aacacccaat tttctgtttc ttggttgtgt ccaaagttat attcaatatc
      301 tggtgttgat gtatcaatat tcttcatccc atcaacaaga gttgatacaa cagccaaatc
      361 ttgtttgatt ctcatcaaat ggtatttctt tcggcgcaat aaactttcaa tggcaagttt
      421 cttcgtcggg aatgcaaaag atctttctgc attttttgct actttcttaa ttgcatatct
      481 atttctcctt tgtttccatt cctgtaacca ctgatttggt actggtttaa aattaacaat
      541 ccaatgcgca ggaaccaacc atgcataatg ctctgtctga tgaaaagcta tatattgaag
      601 tgcgaatatt ttgatcccat cttcttcaac tgtcgcctgg aatctccaga aaacaggcat
      661 tccatcatgt tcagtttctg attcaggaaa aggtacgctc catgattttg tcatatctca
      721 cctcaaataa gtggtttgct gcctaatttc attttctggc gcccaacaca agtcatctcg
      781 ccgtcagttg ttttggtttc cggtagcctg ccgcgtaaat ggctacgttt ggcaggcaaa
      841 tacttccact gcattcatct gccttcttgc agcgaaggct tccgagtgat gctgctttgt
      901 ctgctctgac gcaaccagag agctttagcg caatttttcg cgccagtcgc tgttcttgca
      961 ttgcctgctc acgttgagcc tgtctgcgtg ctctgcggcg atttctggcg ttatcgtcag
     1021 ccagatatgt aatgactact gccatgttga cctccgatga ttgactttgg cggtgacgcg
     1081 ccgggtgctt atcttccggt tgccgtcgtg cagctgcact tctcgtcacc ccaaagccaa
     1141 ctactctttg gttcccgcat ttcggcggga caatcccatc aatgttaaag agcctgccaa
     1201 tctgttccgt ttggctacca gcgacctgct gatgacttaa atttaagatt tcttcaattg
     1261 ttggtcaagg gtgtttttga agaaaactta aatttttatt gcgaggttta agttttgctt
     1321 tgatttttaa aggaaagaaa aaaaggggcg aatgcccctt atggaaggtt tgctattttt
     1381 gcatcgacaa ctacaccgat gattttgcag ttcccgttga tctcaatcat cggatattgt
     1441 gggttaagtg gttttagaaa ccttctacca gcatcaataa ctaacttctt gaaagttgcc
     1501 tcgttttctc cttcgagctt tgccactacc agcttcccat tacgaggttc tacttcagga
     1561 tcgactagta ttatcatccc ttccgggatg ctaagaccgg ctggagccgt catcgaatca
     1621 ccctttacgt ccagccaaaa cgaatcttct gaacaatcta cggttgtatc gtaccagtta
     1681 tctattgcac gcttatgata tggctctaca gcttccatcc aacatcctgc gcttacccaa
     1741 ctaattagag gatacgaacc tcttggatca tgcctgctgt gataggcaat gtttgaaaga
     1801 ctatcttctc ctttcaacaa gtaatcaggg gagcactgta aagccttggc taaagccaat
     1861 aggttttcgc cattgggctc agtttcagaa cgctcccatt gggaaatagc aacattagac
     1921 acgccaacca tcttgccaag ggcagcctgc ctaatcttaa gttcttttct gcgagcgcga
     1981 atacgctcac ccatcagttg tgtattcata gttaagacat cttaaataaa cttgacttaa
     2041 gattcctttg atagataatt taagtgttct ttaatttcgg agcgagtcta tgtacaagaa
     2101 agatgttatc gaccacttcg gaacccagcg tgcggtagct aaagcgttag gcattagcga
     2161 tgcagcagtc tctcagtgga aggaagtcat cccagagaaa gacgcctatc gactggaagt
     2221 cgttacagct ggcgccctga agtatcaaga aagtgcttat cgcaaagcgg cataagcaaa
     2281 ttgctcttta acagtcatgg tccttattcc cgccgaaatg cgggaataca acgcgcatca
     2341 gttggtgcgt ataacttctt atttgttaag gaaatactta catatgcaac ttacaagtac
     2401 tcgcaagaaa gcgaatgcaa tcacaagcaa catcctgaat cgaattgctg tacgtggtca
     2461 gcgaaaggtt gctgatgcat tagggattaa tgaatcgcaa atttcgcgat ggaaagacag
     2521 ctttatccca aagatggcca tgcttctggc tgtgctggag tggggtgttg aagacgagga
     2581 attagcaaag ctagcaaaga aagtagccat ggtgctgaca aaagaaaagc ctcaagactg
     2641 ctgcaacagt tttgaggcct gatgtagaaa gactggatca atccacagga gtcattatga
     2701 caaatacagc
//
LOCUS       DAZFZC010000248         2657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFZC010000248 DAZFZC010000000
VERSION     DAZFZC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.254"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHKXP_005209"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHKXP_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO7649987.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005210"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7649988.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..2648
                     /locus_tag="ACHKXP_005211"
     CDS             1077..2648
                     /locus_tag="ACHKXP_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO7649989.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVQGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          640 a          728 c          738 g          551 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccggacagc ccggctgtgg gtctacgtcc
     1921 gtgatgaccg taacgccggt tcacagatgc ccccggcggt ctggttcgcg tacagtccgg
     1981 accggaaagg tatccatcca caaaatcacc tggccggtta cagcggtgtg cttcaggccg
     2041 atgcttacgg tggttaccgg gcgttatacg aatccggcag aataacggaa gccgcgtgta
     2101 tggctcatgc ccggagaaaa atccacgatg tgcatgcaag agcgcccacc tacatcacca
     2161 cggaagccct gcagcgtatc ggtgaactgt atgccatcga ggcagaggtc cagggctgtt
     2221 cagcagaaca gcgtctggcg gcaagaaaag ccagagccgc gccactgatg cagtcactgt
     2281 atgactggat acagcaacag atgaaaacac tgtcgcgtca ctcagatacg gcaaaagcgt
     2341 tcgcatacct gctgaaacag tgggatgcac tgaacgtgta ctgcagtaat ggctgggtgg
     2401 aaatcgacaa caacatcgca gagaacgcct tacggggagt ggccgtaggc cggaaaaact
     2461 ggatgttcgc gggttccgac agcggtggtg aacatgcggc ggtgttgtac tcgctgatcg
     2521 gcacatgccg tctgaacaat gtggagccag aaaagtggct gcgttacgtc attgaacata
     2581 tccaggactg gccggcaaac cgggtacgcg atctgttgcc ctggaaagtt gatctgagct
     2641 ctcagtaaat atcaata
//
LOCUS       DAZFZC010000249         2655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFZC010000249 DAZFZC010000000
VERSION     DAZFZC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.255"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..316
                     /locus_tag="ACHKXP_005212"
     CDS             <1..316
                     /locus_tag="ACHKXP_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7649990.1"
                     /translation="PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQH
                     KSGNLVPYRVELISRIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSE
                     VA"
     gene            313..984
                     /locus_tag="ACHKXP_005213"
     CDS             313..984
                     /locus_tag="ACHKXP_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO7649991.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTQAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGAWA"
     gene            975..1463
                     /locus_tag="ACHKXP_005214"
     CDS             975..1463
                     /locus_tag="ACHKXP_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7649992.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSAFKNRKRVEKIKH
                     VA"
     gene            1777..1852
                     /locus_tag="ACHKXP_005215"
     tRNA            1777..1852
                     /locus_tag="ACHKXP_005215"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1810..1812,aa:Ile,seq:cat)
     gene            2105..2533
                     /locus_tag="ACHKXP_005216"
     CDS             2105..2533
                     /locus_tag="ACHKXP_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7649993.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQAVINAAYLVACADGECEAS
                     EKAKIEQVLRNQPALSAFTSEINAMSATIIGQLDTNFKTGRRAALREIEDVKHDTREA
                     EDVLDVAVAIAETDGEIEPEERKVLEEIAGVLGLRLENHQ"
     gene            2530..>2655
                     /locus_tag="ACHKXP_005217"
     CDS             2530..>2655
                     /locus_tag="ACHKXP_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7649994.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVL"
BASE COUNT          730 a          587 c          700 g          638 t
ORIGIN      
        1 accatgtatc tcgtgcggaa cgctcacgtc tgctcagtgg gatgccggac attaccggac
       61 aactgctgcg gcacctcaac tccgatttga tgaacgcaat attcacaagc aatgcgtggt
      121 gtgcaaccag cacaaaagcg gaaatctcgt tccgtatcgc gtcgaactga ttagccgcat
      181 cgggcaggaa gcagtagagg aaatcgaatc aaaccataac cgctatcgct ggactgtcga
      241 agagtgcagg gccatcaagg cggagtatca acagaaactt aaaaaactgc gaaacagcag
      301 aagtgaggtt gcatgaatat ctacgaaaga attgatggca gcaaataccg aaatatttgg
      361 gtagttggcg atctgcacgg atgctacacg aacctgatga aaaaactgga gacgatagga
      421 ttcgacacca aaaaagacct gcttatctcg gtgggcgatt tggttgatcg cggtacagag
      481 aacgtagaat gcctggaatt aatcacattc ccctggttca gagctgtacg tggaaaccat
      541 gagcaaatga tgattgatgg cttatcagag cgtggaaacg tcaatcactg gctgcttaat
      601 ggcggtggct ggttctttaa tctcgattac gacaaagaaa ttctggctaa agctcttgcc
      661 cataaagcag atgaacttcc gttaatcatc gaactggtga gtaaaggaaa aaaatatgtc
      721 atctgccacg ccgattatcc ttgtgataaa tacgagtttg gaaagccagt tgatcatcag
      781 caggtaatct ggaaccgcga acgaatcagc aactcacaag acgggatcgt gaaagaaatc
      841 aaaggcgcgg acacgttcat ctttggtcat acgcaagcag tgaaaccact caaatttgcc
      901 aaccagatgt atatcgatac cggcgcagtg ttctgcggaa atctcacatt gattcaggta
      961 cagggagaag gcgcgtgggc ataagagaac taaacctcac caaagaacag catgagtggc
     1021 tgaatggctg gcttgaactg tggggcgcat gggtttattc aggtcgtctg gaaaagcgca
     1081 tgagcagcgt aatagctaaa ttcatggaga gcgtggagcc gggaagagtt atgacaaggc
     1141 caatgtgtaa tgatgatgat ggaatgttga tttctcaggt cgtcgattcc gtcatgtaca
     1201 ttgacaagaa agcctttggc atcctcctca gctactacgc ccacggctct tccaagcacg
     1261 ccattgcatc ttactatcat agcgtcgcaa gacctcgcaa gatgttatgc cggggcggcg
     1321 ggcgcattca aaaaccatcg ctagcaacct gtcgacggga agttgacgaa atcctcaatg
     1381 cctcgttgtt tatgatttac ccgattctgg atagtgcgtt taaaaaccgg aaacgtgtag
     1441 agaaaattaa gcatgtagca tagaacgtgt tgacatcgtt gagcaaatga gcaacactat
     1501 tcgcataagc tgccgttagt gactcttaag ttgcaacggt ggcttttttt atttgggtca
     1561 atcgtataaa ggtcattaga gcctgtaact gtttttcgtc cataaattaa ccttcatttg
     1621 atgctggatt gaacatatca aaatcaggca attacacaaa tctatgtaca ggctctttgt
     1681 gcctgacgtt aaaagatttt ttacaagaat aattttgaat cagtgaattt gtgaactctt
     1741 gcaacattga tttcgtaacg ttattatcct acgctcggcc ctttagctca gtggtgagag
     1801 cgagcgactc ataatcgcca ggtcgctggt tcaaatccag caagggccac caaccgccat
     1861 tagctcatcg ggatagaacg ccagccttct aagcaatcgg tcactggttc gaacccagta
     1921 caacgcacca cacttatttt ccctcgctcg cttttgcgga ccttttttgt atccgcgcca
     1981 cgcccggcgc ataccaacca cagagccttt cgggggggag cttacggagt ggtcagtgtg
     2041 actttctctg tgggcagatc gctcccgggc gttggctcac ccacccaaag gaacgtcacg
     2101 atgtttggta tttttggtaa aaaaagcccg cagagcggca acggaaatta aaaagtttga
     2161 aaaacgcgat ctggcacagg cggtgattaa cgccgcatac ctggtggcct gtgcagatgg
     2221 tgaatgtgag gcttccgaga aagcgaagat cgaacaggta ctgcgtaatc agccagcgct
     2281 gtccgcgttt acgtcagaaa ttaatgcgat gagcgcaacc attatcggtc agctggatac
     2341 gaactttaaa actggtcgtc gtgcggcgtt acgtgagatc gaggatgtga aacacgatac
     2401 gcgtgaagcg gaagatgtgc tggatgtggc ggtggccatt gcggagacag acggcgaaat
     2461 tgagccggaa gagcgcaagg tgctggaaga gattgccggt gttctgggtc ttcgtctgga
     2521 gaatcaccag tgacggtaaa actgcgcctg gctgtggctg cactcctgct gtttctggtg
     2581 gtgatggtgg atttcaccag cagaatcatg tcggtgctgg cggatggggt gctggtctgc
     2641 ggcattgtgg tattg
//
LOCUS       DAZFZC010000250         2638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFZC010000250 DAZFZC010000000
VERSION     DAZFZC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.256"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..273)
                     /locus_tag="ACHKXP_005218"
     CDS             complement(<1..273)
                     /locus_tag="ACHKXP_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFO7649995.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMT"
     gene            466..969
                     /locus_tag="ACHKXP_005219"
     CDS             466..969
                     /locus_tag="ACHKXP_005219"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7649996.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1015..1431)
                     /gene="rraB"
                     /locus_tag="ACHKXP_005220"
     CDS             complement(1015..1431)
                     /gene="rraB"
                     /locus_tag="ACHKXP_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO7649997.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1593..2597
                     /gene="argF"
                     /locus_tag="ACHKXP_005221"
     CDS             1593..2597
                     /gene="argF"
                     /locus_tag="ACHKXP_005221"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO7649998.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPKACWPEAALVTECRALAQQNGGDITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          735 a          609 c          614 g          680 t
ORIGIN      
        1 tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga caactaacaa aaacattccc
       61 accggtaatt ccataagaaa atcgttgtcc gttaacttcc atattagcat agagataacg
      121 cttacatttc attaatgccc atggtaaata gatacctagc gttataatcg ttaacaacac
      181 attcaccaca caaataagaa aatatctctc acctgtgcca tgaaaaacaa acgaatggga
      241 cggaacatcc atttcattaa taacttgagc cataaaaatt ccctgaatta aaaaatccat
      301 tgatataatt tatagataca gacagtaaaa tgttctgcca cgttacaata ctcttatcag
      361 taataggctg gcaaaaactt tattacttta taagtcgctt gcgcacgtaa gaagagagtg
      421 gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa taacattgcg
      481 ccgcaatcac ctgtaatgcg tcgcctgacg cagcaggata atcccgctat cgcccgcgtc
      541 attcgtcagg tatccgccga atacggtctt accgctgata aaggctacac cgtcgccgat
      601 ccgaaccttg acgagttgta tcaagtgtat agtcagcctg gccatgcata ttgggtggtt
      661 gagttcgatg gtgaagtggt cggcggcggt gggattgcgc cattagcagg tagtgaatcg
      721 gacatttgcg aactgcaaaa gatgtatttt cttccggcta ttcgcggtaa agggctggcg
      781 aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa acgctgctat
      841 ctggaaacga ccgctttttt aaaggaagcc attgcgcttt atgagcattt gggctttgag
      901 catatcgact atgcgcttgg ctgcacgggc catgtcgatt gtgaagtgcg gatgctacgc
      961 gaactctaaa aaaatgcccg ctagtgtgtg ctatcgggca ttttaatcaa ttaattagtg
     1021 gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccgtctt cgccgttagg
     1081 atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg ccagcgtcat
     1141 cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc tgaggatgtc
     1201 gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg tcacttcata
     1261 accaagttta aacgcttcaa ctgctgcttt ttccagggtt tccagatcgt ctgcggaaag
     1321 atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta attcttcaat
     1381 aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca tggctcattc
     1441 ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc tccacctttg
     1501 tgacaaagat ttatgcttta gacttgcaaa tgaataacca ttcatataaa ttgaatttta
     1561 attcattgag gcgttagcca caggagggat ctatgtctgg gttttatcat aagcatttcc
     1621 tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta gccgcgaagc
     1681 tgaaagccga taagaaaagt ggtaaagaag aagccaaact cactggtaaa aacatcgcgc
     1741 tcatcttcga aaaagactcg actcgtaccc gatgctcttt cgaagttgcc gcatatgacc
     1801 agggtgctcg cgttacttat ctcggcccaa gcggcagcca gattggtcat aaagagtcga
     1861 ttaaagacac tgcccgcgtg cttggtcgca tgtatgacgg tattcagtat cgcggctatg
     1921 gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccagtatgg aacggcctga
     1981 ccaatgagtt ccaccccacc cagctgctgg cggatcttct caccatgcaa gagcatttgc
     2041 ccggtaaaac attcaacgaa atgacgctgg tctatgcagg tgacgcacgt aacaacatgg
     2101 gaaactcgat gcttgaagct gcggcgctta ccggtctgga tttgcgtctg gtcgcgccaa
     2161 aggcatgctg gccggaagct gcgctggtta cggaatgccg cgccctggca cagcaaaatg
     2221 gcggggatat tacgctgacg gaagatgtag ccaaaggagt tgaaggtgct gactttatct
     2281 ataccgatgt atgggtgtcg atgggtgaag caaaagagaa atgggcggaa cggattgcat
     2341 tgctgcgtga ttatcaggtg aacagcaaga tgatgcagtt gactggtaac ccggaggtca
     2401 aattcctcca ctgcctgccc gcgtttcatg acgaccaaac gacgcttggt aagaaaatgg
     2461 cggaagaatt tggcctacat ggcggaatgg aagtgactga tgaggtcttc gaatctgccg
     2521 ccagcattgt ttttgatcag gcggaaaacc gtatgcatac catcaaagcg gtgatggtcg
     2581 cgacgctcag taaataattg atatcgctat aaagatgcag ggaaatacaa aactatcc
//
LOCUS       DAZFZC010000251         2621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFZC010000251 DAZFZC010000000
VERSION     DAZFZC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.257"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..1266
                     /locus_tag="ACHKXP_005222"
     CDS             join(53..344,344..1266)
                     /locus_tag="ACHKXP_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7649999.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGSGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKIMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     KEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVAFIIDVFAGYIVGWQVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNNDLAA"
     misc_feature    334..450
                     /locus_tag="ACHKXP_005222"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(1325..1447)
                     /locus_tag="ACHKXP_005223"
                     /pseudo
     CDS             complement(1325..1447)
                     /locus_tag="ACHKXP_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1481..1942)
                     /locus_tag="ACHKXP_005224"
     CDS             complement(1481..1942)
                     /locus_tag="ACHKXP_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7650000.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(1935..>2621)
                     /locus_tag="ACHKXP_005225"
     CDS             complement(1935..>2621)
                     /locus_tag="ACHKXP_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO7650001.1"
                     /translation="AERKRAQRERERKREQNGDCHGASRNVTHMSRQVTTDKDTDTEL
                     NPTHNARMRESAPTGESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSSDFRQRAAV
                     WGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSH
                     AASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDTEERGRTFE
                     ALDCPDWRDD"
BASE COUNT          570 a          735 c          686 g          630 t
ORIGIN      
        1 aaccgccccg gaaatcctgg agactaaact ccctgagaaa gaggtaaaca ggatgactaa
       61 aaatactcgt ttttcccccg aagtccgtca gcgggcgatt cgtatggttc tggaaagtca
      121 ggatgaatat gactcacagt gggcggcaat ttgttccatt gccccaaaga ttggctgtac
      181 gccggagact ctgcgtgtct gggttcgcca gcatgagcgg gataccggga gtggtgatgg
      241 tggactcacc accgctgaac gtcagcgtct gaaagagctg gaacgtgaaa atcgtgaact
      301 gcgccgcagt aacgatatcc ttcgccaggc ttccgcttat tttgcgaagg cggagttcga
      361 ccgcctctgg aaaaaataat gccactgctg gataagctgc gtgagcagta cggggtcgga
      421 ccggtatgca gcgaactgca tattgccccg tcaacgtatt accattgtca gcaacagcga
      481 catcatccgg ataaacgcag tgcccgtgcg cagcacgatg actggctgaa gaaagagata
      541 cagcgcgtat acgatgaaaa tcaccaggta tacggtgtgc gtaaagtctg gcgtcagttg
      601 ttacgggaag gtatcagagt ggccagatgc actgtggcac gtctcatggc agttatggga
      661 cttgccggtg ttctccgggg taaaaaggtc cgcactaccg tcagccggaa agccgttgcc
      721 gcatgcgacc gcgtaaaccg tcagttcgtg gcagaacgtc cagaccagtt gtgggtggct
      781 gattttacct gggtaagcac atggcagggc ttcgtttatg tggcgttcat cattgatgtg
      841 ttcgccggat acatcgtggg atggcaggtc tcatcatcca tggaaacaac attcgtgctg
      901 gatgcactgg agcaggcgtt gtgggcccgt cggccgtccg gcacaatcca tcacagtgat
      961 aaaggttctc agtatgtatc gctggcctac acgcagcggc ttaaggaagc cggattactg
     1021 gcatcaacag ggagtactgg cgactcgtat gacaacgcga tggctgagag catcaatggt
     1081 ctttacaaag cggaggtaat acaccgtaag agctggaaaa accggacaga agtggaactg
     1141 gccacactaa cgtgggtgga ctggtataac aatcgacgat tgctggaaag gctgggccat
     1201 attcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaataa tgatctggca
     1261 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcaca gttttatcaa
     1321 cgacgccatt tcttttgagt tcccacagct cgttgacagc ctcttcacga ctgattccaa
     1381 ggctggccgc cactacctgt gaagaggctc ttttcagtgc tttcagtgcg tcaaatacgg
     1441 tttccattaa tatttcctcc gacaaaatcg tttctcagat tcaaataaaa ccagctgcct
     1501 tccggcgttc gtattcctgt ttcagccgct caattggcgt tggcccttgc gggtgtttcg
     1561 ccccttccag ttgtcgtcgc actggcggaa cactcatccc gttaccaaca tgctttgccc
     1621 atttcgtcag ttgccgttcc gcaagtcgtt ttaactcacc ctgcgtcatc tggcgctcaa
     1681 tccctctggt acgcatttcg aggcagatgt ggtacagcac aggctgtggc cacgggtatt
     1741 tatcactccc gtcgtatcgc caggattcat tgcgccagcg ccggtactct tccatcactg
     1801 catccaccgt aagaccaaat ggatttgccc cactctccga aatcagtgca acaaactcag
     1861 ccaggtccgg gggccacgtt tcacccgccc ggcagcggtc catgcactga cggcagacca
     1921 gccggatttg ctgttcagtc atcgcgccaa tctgggcaat ccagagcttc gaaggtgcgg
     1981 ccccgttctt ctgtgtccag cggttcgaat acacctcccc cataagctcc cacagcttcc
     2041 aggccgtttc cgttgctgat aaatccgttg tcacgttccc actgttcgcg tgctgcccgg
     2101 atttcctgaa ctgcccgtga tgccgtgcca cctgatgctg catggcttac ccccttgctg
     2161 actggtttta cctgtgccct gacgtgctgc acgtggcggg caaatttctg ctcccactga
     2221 acctgcgtga aaaccttccc ctccgccatc cagtaatccc ggaatgcggc aagctctgca
     2281 ggtgtaaatt ccggctcagg cagagccata ccccacactg ctgcccgttg tcgaaaatcc
     2341 gacgacggct gccagacagt agtcatcgaa aatttcccga tcggttcgct caggccgtcc
     2401 aggtattcag gttcggctgt ctgcaacggc gcaccatgcg actcaccggt tggagcactc
     2461 tcgcgcatgc gcgcgttatg tgtggggttt aattctgtat ctgtatcttt atctgtcgtg
     2521 acttgtcgtg acatgtgcgt gacatttcgt gacgcgccgt gacaatcgcc attttgttcc
     2581 cgctttcttt ccctctcacg ctgcgccctc ttgcgctctg c
//
LOCUS       DAZFZC010000252         2610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFZC010000252 DAZFZC010000000
VERSION     DAZFZC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.258"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            41..310
                     /locus_tag="ACHKXP_005226"
     CDS             41..310
                     /locus_tag="ACHKXP_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7650002.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHGQSLTVQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            423..998
                     /locus_tag="ACHKXP_005227"
     CDS             423..998
                     /locus_tag="ACHKXP_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7650003.1"
                     /translation="MPLTSAIASYSFSTGMQVLRAQMVASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            1289..1870
                     /locus_tag="ACHKXP_005228"
     CDS             1289..1870
                     /locus_tag="ACHKXP_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO7650004.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            1938..2573
                     /locus_tag="ACHKXP_005229"
     CDS             1938..2573
                     /locus_tag="ACHKXP_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7650005.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTSNKILMEKINSCVFRPDSNHFSCPESFLTCPITLDTPENGVFMRNSRGAEI
                     CSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFDSKKEAFVASDA"
BASE COUNT          761 a          439 c          672 g          738 t
ORIGIN      
        1 gatgaagtgg gcagtgatac aggcagaaca ggagaatgac atgaacatac taaaaaaact
       61 tatgcagcgt ctgtgcggtt gcggaaagca tgatggccgt gaacacgggc agtcgcttac
      121 agtacaactg cgactggggc cggcagacat cctggagtca gatgagaatg gcattattcc
      181 ggagcaggcc agggtaatca cgcaggtggt gatactggat gcggataaaa agcagataca
      241 gtgcgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa atattggcgg
      301 aatgaaatag ccgacagctt cacaaaaacc ggagtccggc tccggttttt gttgtcatgt
      361 atgggggctg tttgttatga ctccctgtgt ttggaatgaa tatttaaata gggagttttg
      421 tcatgccgtt aacatcagct attgcatcct attcattttc caccggaatg caggttcttc
      481 gtgctcaaat ggtcgccagt ggcggtggag aaattacagt aggcgggcag acggtccgta
      541 tcacatatag tgaaacggat ggtcgctttc tggcgagtgg gggcaataac agtttgcttt
      601 ctggattatt acttacaggg cttaatggtg gtcctgaagc gctcagggat ataatgttaa
      661 gaatggtttc aggttcaggt aacacacaat cacatggtga tattgagggg aaaatatcac
      721 aatgtaagtt ttctgttaat acggagagcc ttcagtgtcc atccgaggcg gttcgatgcc
      781 caattatact ggataaacca gaagaaggtg tgtttgttaa aaattcagaa ggttctttgg
      841 tttgtacctt atttgattcg gtttcttttt ctcatttggt tcgcgacggt gggaagcacc
      901 cgctaacacg agaaccaata acgtcatcaa tgattgtaag tcaagaacaa tgtatttatg
      961 accaaaccaa aggaaacttt gtcataaagg ataagtgaaa taaatattac ccaagctata
     1021 tgttaactgc cagttgctta tatgaaatgc tacagatgtt cagggtataa ggatgtggta
     1081 attggtgtac tggatgcagc taaaaagcat atacagtgag tggtgaggcc gctgcaaatc
     1141 tggcgtgctg acggggaaat attggcggga tgaaatagcc gacggattca caaaaaccgg
     1201 agtccggctc cggtttttgt tgtcatgtca gggagatgtt tgttatgaag cccagaggaa
     1261 atatttatct gtatgaagga atatggtaat gcctggatta gtatcatata tatcatcgac
     1321 ttcattcgcg aatgagatgg cggaaatgcg tcagcaggta atggaagggc agattggagg
     1381 atttctcctg ggaggggaga gagttagagt ttcttatatg ccagatacag gccgtttttt
     1441 agcagaaagt gaagggctgg gactggttta tgcagaatta ttgaatattg gttttaatga
     1501 tggagttgat gcgctcagaa acagggtgtt aagcgtgctt cctggaatgg tggcgcagcg
     1561 acaagagaac tctttgcagg ccaaaatatc ggaatgtacc tttactgttg atattgaaaa
     1621 acttcactgt cctggtgagg tgcttcaatg tccaattaca ctggagcagc ctgaaaaagg
     1681 tatttttgtg aagaattcag atggttcaga tgtatgtact ttatttgatg ccgctgcatt
     1741 ttctcgtttg actggtgaag gcttacccca cccactgacc cgggaaccaa taacggcatc
     1801 aataattgta aaacatgaag aatgcattta tgacgatacc agaggaaact tcgttataaa
     1861 gggtaattga aatgaacatt accctttatt taatgaaaca tcctgcaaac tgatatgaat
     1921 tactgaatga ggtttttatg cctgttacca ccttaagtat cccaagtata tctcaattat
     1981 ctcctgcagg agtgcagtct ttgcaggatg cagccagact tgaaagtgga ataagaatat
     2041 ccattggtag tggccaatat tctgttcact atgtccaact actggatgga ttttcagttg
     2101 aaccggtgag aggaggctta ctggataggc tattggggcg tgagcatcga atggagagaa
     2161 gggctgtggc tctggaaagg caattaaatg gaggtgtcga ttttttaagt agtgttaata
     2221 actattttca gagtgtcatg gcagaacaca gagaaaataa aacaagtaat aaaatattaa
     2281 tggaaaaaat aaattcttgt gtatttagac cggattctaa tcacttttct tgcccggagt
     2341 catttttgac atgcccgata acgctggaca cacctgagaa tggagtgttc atgagaaact
     2401 cacgaggtgc tgagatatgc tctctatatg ataaggacgc gttagtgcaa cttgttgaaa
     2461 ctggtggagc tcatcctctg agtcgagaac ctataacaga atcaatgatc atgagaaaag
     2521 acgaatgtca ctttgattca aaaaaagaag cctttgttgc aagtgatgct taattttttc
     2581 tgttggtgtg tttttatatt aatagtttat
//
LOCUS       DAZFZC010000253         2601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFZC010000253 DAZFZC010000000
VERSION     DAZFZC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.259"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..837)
                     /locus_tag="ACHKXP_005230"
     CDS             complement(<1..837)
                     /locus_tag="ACHKXP_005230"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7650006.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DEL"
     gene            complement(827..2419)
                     /locus_tag="ACHKXP_005231"
     CDS             complement(827..2419)
                     /locus_tag="ACHKXP_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7650007.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEEKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQKIAESRRPEEETTPRET"
     gene            complement(2416..>2601)
                     /locus_tag="ACHKXP_005232"
     CDS             complement(2416..>2601)
                     /locus_tag="ACHKXP_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO7650008.1"
                     /translation="QALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELNRAINDAE
                     SVLGTTRRRRRPLGVRL"
BASE COUNT          579 a          860 c          631 g          531 t
ORIGIN      
        1 taattcatcc gccagcccgg catcaatgcc ggactgacct tcaaaaacgg cggcctctgt
       61 tcccgtgacg gcatcaacag acagaccggt aaacatggcc actttttcgg caaacatccg
      121 gcgcgccgca tcaatgcgct gctgcatgtc ctggcgaacc tctgccggta aggcttcaaa
      181 ctgattgcca tccaccttgt gcgcccctga gtaaatcagc gtgatatcca caccggcctg
      241 cgccagatga ccggcatagc tgacatggct catcatcacg ccaatggagc cgatacggga
      301 tgtctgggta accagccgtc gggagcaggc cgacgccagc agcatggctg cagaacaggc
      361 cgtgtcattg cacagtgccc agaccggctt ctgctgacgg aggcggtaaa tcatgtcagc
      421 gcagtcaaac gcgccggcgg cctgcccgcc cggactgtca atgtccagca gtatgccccg
      481 cacctggcta tctgccattg cctgctgaag acaggcgaca atgccgtcat agccagtcat
      541 tccggaaaat ggccgcatcc cccccagccg gtgcaccagc gtgccggtca ccggcagtac
      601 cgcaataccg ttcaccaccc ggtaaacacg ggccggtcgt ttacctccgg ccatgtactc
      661 gtccgtttca gccagcattc cgggagcatc aagctgtacc tgctgttgtg gtaccgaaag
      721 acttgctgcc cccatctcgc gcccgagcgc gcaaaagaaa acccgcgcat aggcgggctc
      781 cagaagcagc ggttcattga atgctgcggc aataatgtgt gaaagattac gtctcacgtg
      841 gtgttgtctc ctcttccggc ctgcgactct ccgctatctt ctgctgatac gcctgcgcta
      901 tccacaccgg acgtgagagt ccggcttttt cccgctctgc agattccctg acctgctggc
      961 ggaaaatgtc ctgataatcc tcacccatca gcgccagctc tttctcatac gtgctcagtc
     1021 cggcctcaat gcgcatcact gattcctgaa cctccttgag cccgtcaatg gccattcttc
     1081 cggctccaat ccactcagcc cgtgaccagg ctgatcgcgc ctgataaaaa tcaaaacgtg
     1141 cccgtggcgg acgaataatc ccccgaagaa gtgcctcttc cagccagcag gaaaacatct
     1201 gcgtggccag ccgggacgca ataaattttc gccgccccat aaaatagcgc cacgactcat
     1261 tggcggatgc gcgggcactt gaataactga ccttcgagta atcacgggac aactgttcgt
     1321 aggaaacgcc aagaccggcg gcgatatacc gcagcagcgc ctgttcaagc gccgaaaatc
     1381 cattgtctga atcctgcgcg gtctgaagtt tcagatcatc accggggaaa aggtgcggaa
     1441 ttttgacacc acccagcgtc acgctattcg tgtcatacca ggtggagaac ttatccagaa
     1501 tattaataag cggattatcc ttctgcccct gcggcgcacc ggcgatatat tcaaaggcct
     1561 tttcggtatc aaggtcactt tcaatcgtcg ctgcatacat cgccttcact atggccgact
     1621 gaagctgtgt tgcctgcagg gaatcgagca tcttcagccg ttccatgacg ctgtaaaact
     1681 gattggcccc acgggtctgc ccgtcctcca ccggctcgaa aatatgcagc atggccggac
     1741 gcccggtggg aagttcacgc gggatccgtt cccatcgtcc actcccggag aacggaaaat
     1801 catcctcaca gatatggtac gcaacggcac gaccatatcg atcgacctcc acaccggccc
     1861 gcagaaaacg gttccccata ccgtgtcctg gcgtgtccac ccgtttcgga ctcacggctt
     1921 taaaacgcgt acgaaacagt tgcgtgctct ccgtatccca gaccggctgc acaaagattt
     1981 cgccgttaaa cgcatgaacg cccacacctt cacgaataaa ttccgtaaac gtgcgtttct
     2041 cttccacgtc gatctcgcca aacatccctt cggcgtattc cgaccaggcc gcctccacct
     2101 catcgacaaa actttttgcc gcagtctccc gcatccccag ccagcgccag ttcggacggt
     2161 agctgatcag aaacatatgc ccgacaatat gatccttatg cagggccacc gcattggccg
     2221 ctatcccgtt attgcgcacc agatcatctg cccgggcatt ccccagacgc aacgcaggca
     2281 gcagggctgc atcggcactc tgcgccggtg gcaaccactc cgccatttgc ccgccaaatc
     2341 ctgcaccgcc cccgttgtag ctgagactct cacgaagcgg aacgccgttc acatcaatca
     2401 ggacaggcgt tcgtttcata acctcactcc cagcggacga cggcgacgcc gggttgtccc
     2461 cagtaccgac tccgcatcat tgatcgcccg gttaagctca tccagagaag ccgccgtata
     2521 ttcaattctg cgaccatctt tctggacaga caccacccgt ttaccggtta ataaatcaag
     2581 gcgcgcctga cgcagcgcct g
//
LOCUS       DAZFZC010000254         2601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFZC010000254 DAZFZC010000000
VERSION     DAZFZC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.260"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..837)
                     /locus_tag="ACHKXP_005233"
     CDS             complement(<1..837)
                     /locus_tag="ACHKXP_005233"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7650009.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DEL"
     gene            complement(827..2419)
                     /locus_tag="ACHKXP_005234"
     CDS             complement(827..2419)
                     /locus_tag="ACHKXP_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7650010.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEEKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYEQQIAESRRPEEETTPRET"
     gene            complement(2416..>2601)
                     /locus_tag="ACHKXP_005235"
     CDS             complement(2416..>2601)
                     /locus_tag="ACHKXP_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO7650011.1"
                     /translation="QALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELNRAINDAE
                     SVLGTTRRRRRPLGVRL"
BASE COUNT          578 a          855 c          631 g          537 t
ORIGIN      
        1 taattcatcc gccagcccgg cctcaatgcc ggactgacct tcaaaaacgg cggcctctgt
       61 tcccgtgaca gcatcaacag acagaccggt atacatcgct actttttcag caaacatccg
      121 gcgcgccgca tcaatgcgct gttgcatgtc ctggcgaacc tctgccggca acgcttcaaa
      181 ctgattgcca tccaccttgt gcgcccctga gtaaatcagc gtgatatcaa caccggcctg
      241 cgccagatga ccggcatagc tgacatggct catcatcacg ccaatggagc cgatacggga
      301 tgtctgggta accagccgtc gggagcaggc cgacgccagc agcatggctg cagagcaggc
      361 cgtgtcattg cacagtgccc agaccggctt ctgctgacgg aggcggtaaa tcatgtcagc
      421 gcagtcaaac gcgccggcgg cctgcccgcc cggactgtca atgtccagca gtacgccccg
      481 cacctggcta tccgccattg cctgctgaag acaggcgaca atgccgtcat agcctgtcat
      541 tccggaaaat ggccgcatac cccccagccg gtgcaccagc gtgccggtca ccggcagtac
      601 agcaataccg ttcaccaccc ggtaaacacg ggccggtcgt ttacctccgg ccatgtactc
      661 gtccgtttca gccagcattc cgggagcatc aaactgtacc tgctgttgtg gtaccgaaag
      721 acttgatgcc cccatctcgc gcccgagcgc gcaaaagaaa acccgcgcat aggcgggctc
      781 cagaagcagc ggttcattga atgctgcggc aataatgtgt gaaagattac gtctcacgtg
      841 gtgttgtctc ctcttccggc ctgcgactct ccgctatctg ctgctcatac gcctgcgcta
      901 tccacaccgg acgtgagagt ccggcttttt gtcgctcagc agattccctg acctgctggc
      961 ggaaaatgtc ctgataatcc tcgcccatca gcgccagctc tttctcatac gtgctcagtc
     1021 cggcctcaat gcgcatcact gattcctgga cttccttgag cccgtcaatg gccattcttc
     1081 cggctccaat ccactcagcc cgtgaccagg ctgatcgcgc ctgataaaaa tcaaaacgcg
     1141 cccgtggcgg acgaataatc ccccgaagaa gtgcctcttc cagccagcag gaaaacatct
     1201 gcgtggccag ccgggacgca ataaattttc gtcgtcccat aaaatagcgc cacgactcat
     1261 tggcggaggc gcgggcactt gaataactga ccttcgagta atcccgggac aactgttcgt
     1321 aggaaacgcc aagaccggcg gcgatatacc gcagcagcgc ctgttcaagc gccgaaaatc
     1381 cattgtctga atcctgcgca gtctgcagtt tcagatcatc accggggaaa aggtgcggaa
     1441 ttttgacacc gcccagcgtc acgttattcg tgtcatacca ggtggagaac ttatccagaa
     1501 tattaataag cggattatcc ttctgcccct gcggcgcacc ggcgatatat tcaaaggcct
     1561 tttcggtatc aagttcactt tcaatcgtcg ctgcatacat ggctttcaca atggccgact
     1621 gaagctgtgt tgcctgcagg gaatccagca tcttcagccg ttccatgacg ctgtaaaact
     1681 gattagcccc acgggtctgc ccgtcctcca ccggctcgaa aatatgcagc atggccggac
     1741 gcccggtggg tagttcacgc gggatccgtt cccatcgtcc actaccagag aacggaaaat
     1801 catcctcaca gatatggtac gcaacggcac ggccatatcg atcgacctcc accccggccc
     1861 gcagaaaacg gttccccata ccgtgtcctg gcgtgtccac ccgtttcgga ctcacggctt
     1921 taaaacgcgt acgaaacagt tgcgtgctct ccgtatccca gaccggctgc acaaagattt
     1981 cgccgttaaa cgcatgaacg cccacacctt cacgaataaa ttccgtaaac gtgcgtttct
     2041 cttccacgtc gatctcgcca gacatccctt cggcgtattc cgaccaggcc gcctccacct
     2101 catcgacaaa actttttgcc gcagtctccc gcatccccag ccagcgccag ttcggacggt
     2161 agctgatcag aaacatatgc ccgacaatat gatccttatg cagggccacc gcattggccg
     2221 ctatcccgtt attgcgcacc agatcatctg cccgggcatt ccccagacgc aacgcaggca
     2281 gcagggctgc atcggcactc tgcgccggtg gcaaccactc cgccatttgc ccgccaaatc
     2341 ctgcgccgcc cccgttgtag ctgagactct cacgaagcgg aacgccgttc acatcaatca
     2401 ggacaggcgt tcgtttcata acctcactcc cagcggacga cggcgacgtc gggttgtccc
     2461 cagtaccgac tccgcatcat tgatcgcccg gttaagctca tccagagaag ccgccgtata
     2521 ttcaattctg cgaccatctt tctggacaga caccacccgt ttaccggtta ataaatcaag
     2581 gcgcgcctga cgtagcgcct g
//
LOCUS       DAZFZC010000255         2565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFZC010000255 DAZFZC010000000
VERSION     DAZFZC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.261"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1309
                     /gene="escC"
                     /locus_tag="ACHKXP_005236"
     CDS             <1..1309
                     /gene="escC"
                     /locus_tag="ACHKXP_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO7650012.1"
                     /translation="KVLEKLSRLYHLTWYYDENILYIYKTNEISRSIITPTYLDIDSL
                     LKYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYITSLSESLDKEAQSKAKNKDV
                     VRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNGSLPSTGKGAVERNGNLFDN
                     SVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQIEISVSIIDVDANDLQQLGV
                     NWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRVNALQQNSKAKILSQPSIIT
                     LNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTPRILDDNSNSLTGKRRERVR
                     LLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLSAGESLLLGGFIQDKESSSK
                     DGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKSASSE"
     gene            1323..1778
                     /gene="cesD"
                     /locus_tag="ACHKXP_005237"
     CDS             1323..1778
                     /gene="cesD"
                     /locus_tag="ACHKXP_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO7650013.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(2164..>2565)
                     /gene="grlA"
                     /locus_tag="ACHKXP_005238"
     CDS             complement(2164..>2565)
                     /gene="grlA"
                     /locus_tag="ACHKXP_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO7650014.1"
                     /translation="NKNGDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDIAEAF
                     GINLRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPVST
                     GPKRKTYRVGNGIVGQSNIWNEMIMRRKKEN"
BASE COUNT          842 a          496 c          475 g          752 t
ORIGIN      
        1 gaaagtactg gaaaaactgt ccaggctcta tcatctcaca tggtattatg atgagaacat
       61 tttgtatatt tataaaacaa atgaaatatc aagaagcata attacgccaa catatctgga
      121 tattgactca ctgttaaaat atctgcgtga tacaatctca gtaaataaaa actcctgtaa
      181 cgtgagaaaa atcacaacat ttaactcgat tgaagttcgt ggcgttcctg aatgcataaa
      241 atacattaca tctttatctg aaagtcttga taaagaagca cagtctaaag caaaaaataa
      301 agatgtcgtt agagtattta aattaaacta tgcatcagcg acagatataa cgtataaata
      361 ccgcgaccaa aatgttgtcg tgcctggcgt cgtctccatc ctgaagacga tggcaagtaa
      421 tggctcactt cccagcacag gcaaaggcgc tgtagaaaga aacggcaacc tgtttgataa
      481 cagtgtaaag atttccgcag atccacgtct taatgcagtt gttgtaaaag atcgtgaaat
      541 aactatggac atatatcggc aattaatttc tgagcttgat attgagcaaa gacaaattga
      601 gatttctgtt tcaattattg atgttgatgc aaatgatctt cagcaactgg gagtaaattg
      661 gtccggtaca ttaaatgcag ggcaaggttc tattgccttt aacagttcaa cggcgcaagc
      721 taatctcagt tcttctgtga taagcaacgc caataatttc atgatcagag tgaatgcgtt
      781 acaacagaac tcgaaagcaa aaatactctc tcagccatca attataacct taaataatat
      841 gcaggcgatt cttgataaaa acgtaacgtt ttatacaaaa gtttccggag aaaaggtggc
      901 gtctctggaa agtattacca gcggaacatt gctgagagtg acaccacgta tacttgatga
      961 caactcgaat agtcttaccg gaaaacgtcg ggaacgagtt cgtttgttac ttgatattca
     1021 ggatggcaat caatcgacta accaaagtaa tgctcaggat gccagcagca tcttacctga
     1081 agtacaaaat tcagaaatga caacagaagc gacattaagc gctggagaaa gtctgctgct
     1141 tggcggattt atccaggata aagagagttc aagtaaagat ggtataccgt tgttaagtga
     1201 tattcctgtt attggtaact tattctccag tacgacaaaa caaaaacaca gcgttgttcg
     1261 actctttttg attaaagcga ccccgataaa atcagcatct agcgaataat aaatgaggct
     1321 ttatgagcag gaaatttagc tctctacagg atatctatga tttctaccag gatggtggta
     1381 cgttagcatc actgacaaat ttgacgcaac aagatctcaa tgatcttcat tcttatgcct
     1441 atacagcata tcaatctggt gatgttataa ccgcaagaaa tctatttcat ctgctaacat
     1501 atctggagca ctggaattat gactacaccc tatctctggg gttgtgtcat cagcgtttat
     1561 caaatcatga agatgcacaa ctgtgtttcg ctcgctgtgc aactttagtt atgcaagatc
     1621 ccagggcatc ttattattct gggattagct acttattggt cggaaatcgg aaaatggcca
     1681 aaaaagcctt taaggcttgt ttaatgtggt gtaatgaaca agaaaaatac gctacatata
     1741 aagaaaatat taaaaaattg ttaggtaata cagattaaaa actaccatct gccataccaa
     1801 tccccaggct gtctggggat tttctatcgg aaataataaa gctgctttgg tgaagtgcgc
     1861 ccaacgttga gcatcgaccg ctggcagatt tatctacagg tgtgggttac tccattaacc
     1921 aggattttat gcgcctgaac catcttctgc tgtactcaat gtcatttccg cttatggaat
     1981 aagcagtata tatcttaact tagcatcaga aatctgactg tttcaatcat ctatagccca
     2041 gagtgtattt gtcaggaaat gactttcaaa ctttggcaga gaaaaagact tatcctggaa
     2101 aacaaagccc ttgaatagag ctttattttt attcttctat aaaatatact caaaaaacta
     2161 cgtttaattc tcctttttcc gcctcatgat catttcgttc cagatattag actgccccac
     2221 aataccatta ccgactcggt aggtttttct ttttggtccg gttgataccg gactttctat
     2281 cggaacagcc tcgaggttaa catcataaat gaaaatctca aggcgcttat aatgcaagtt
     2341 accataacta acatatctga cacgaaatga aattttttct tttcttctcg atatataagt
     2401 tataataaat gatgctctcc tcaggtttat accgaatgct tcggcaatat cattgcgaga
     2461 aatccatttc tcctgcaatt tacaccaaag agaaaccaag atatacagag gctcaccatc
     2521 gtaattcttt actgagtcag gaattacata gtcgccattt ttatt
//
LOCUS       DAZFZC010000256         2513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFZC010000256 DAZFZC010000000
VERSION     DAZFZC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.262"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1950
                     /locus_tag="ACHKXP_005239"
     CDS             <1..1950
                     /locus_tag="ACHKXP_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7650015.1"
                     /translation="REVTLPETGTSTVNLINGSGKPVSVDITAHPAPDRIQVSTLPDG
                     VATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSG
                     TLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEGSRLVTT
                     AITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQ
                     ITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFW
                     FYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEM
                     AEMKTSITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGI
                     PYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNTTPLFVAQGNQLFMNDVFLKR
                     LFAVSITSSGNPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSA
                     NQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHER
                     EHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPVGHGHMTLEFSVSAWLVNN
                     WYPTASISDLLVVVMKKSTAGISIS"
     gene            2017..>2513
                     /locus_tag="ACHKXP_005240"
     CDS             2017..>2513
                     /locus_tag="ACHKXP_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7650016.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQ"
BASE COUNT          595 a          637 c          757 g          524 t
ORIGIN      
        1 cgtgaggtga cactgccgga gacaggtaca tcgacggtga acctgattaa cggcagcggt
       61 aagccggtga gtgtggacat caccgcacac cccgcgccgg accggataca ggtcagtacc
      121 ctgccggatg gcgtggcgac atacggtgtg tggggactct ccctgccgtc actgcgtcgt
      181 cgcctgttcc gctgtgtttc catccgggaa aacacggacg gcacctttgc catcacggcg
      241 gtgcagcacg taccggaaaa ggaagccatc gtggataacg gggccagctt tgagccgcag
      301 tcaggcaccc tgaacagcgt catcccgccg gcagtgcagc acctgacggt ggaggtgagc
      361 gcagctgacg gccagtatct ggcacaggcg aaatgggaca cgccgcgggt ggtgaagggg
      421 gtgcgcttca gtctgcgact gaccagcgga agcggagaag gcagccgtct ggtgaccacc
      481 gccatcaccg cggatacaga gcatcgttcc agtggtctgc cgctcgggga atacaccctg
      541 acagtcaggg caattaacag ttatggccag cagggcgaac cggccaccac caccttccgg
      601 attaacgcgc cagcaaaacc cgccaccatt gaactgacgc cggggtattt tcagataacg
      661 gcggtcccgc gtcttgcggt gtatgacccg acggtacagt ttgagttctg gttttcggag
      721 acaaaaatcg cagacacatc tcaggtggaa acctctgccc gttatctggg gaccggcagt
      781 cagtggagtg tatccggccc gcacattaag cccgggaagg atttctggtt ttacgtgcgc
      841 agcgtcaacc tggtggggaa atctgctttt gtggaagcca gtggccgggc cagcaatgat
      901 gcagaagggt atctggggct gtttcgggaa aaaataggaa aactgcatct ggctcagggg
      961 ctgtgggagc tgatagacaa cagccagctt gcggatgaga tggcggagat gaagacctcc
     1021 atcaccgaaa cccgcaatga aatcacacag acggtcagta aaacgctgga ggaccagagc
     1081 gccaccatac agcagataca gcgcgtgcag aaggacacaa atgatgacct tgctgcactt
     1141 tacatgctga aggtacagaa aacaaaaaat ggcataccct atgttgccgg tattggagcg
     1201 gggattgagg atactgatgg ccagcccctg agcaacatac tgctgctggc tgaccgtatt
     1261 gcgatgatta acccggagag cggcaacacc acgccgttat ttgtggcgca ggggaatcag
     1321 ttgttcatga acgatgtgtt cctgaagcgg ctgtttgcgg tgagtatcac ctcgtccggc
     1381 aatcccccga cgttttccct gacgccggag ggcaggctga ccgcaagaaa tgctgatatc
     1441 agcggtaacg tgaatgcgaa ttccgggacg ctcaacaacg tcacgattaa cgagaactgt
     1501 cgggttctgg gaaaactgtc cgcgaaccag attgaaggcg atctcgttaa aacagtgggc
     1561 aaagctttcc cccgggattc ccgtgcaccg gagcggtggc catcagggac cattaccgtc
     1621 agggtttatg acgatcagcc gtttgaccgg caaattgtta ttccggcggt ggcattcagc
     1681 ggcgctaaac atgagagaga gcatactgat atttactcct catgccgtct gatagtgcgg
     1741 aaaaacggtg ctgaaattta taaccgtacc gcgctggata atacgctgat ttacagtggc
     1801 gttattgata tgcctgtcgg tcacggtcac atgacgctgg agttttcggt gtcagcatgg
     1861 ctggtaaata actggtatcc cacagcaagt atcagcgatc tgctggttgt ggtgatgaaa
     1921 aaatccacag caggtatcag tatcagctga attttataac ccagaacggg cgtcagaaat
     1981 gacgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaacttta tgccgccatt
     2041 ttgtccgcag ccatttgtct ggccgtatcc ggtgcgcctg catgggcgtc tgaacatcag
     2101 tccacgctga gcgcggggta tcttcatgtc tcgacgaacg ttcctggcag cgatgaactg
     2161 aacgggatta acgtgaaata ccgttatgag tttacggaca cactggggat ggtgacgtcg
     2221 ttcagctatg caggagacaa gaatcgccag ctgacccatt acagcgatac ccgctggcat
     2281 gaagattccg tgcgtaaccg ctggttcagc gtaatggcgg ggccgtctgt gcgcgtgaat
     2341 gaatggttca gcgcgtatgc gatggcgggt gtggcttaca gccgtgtgtc gactttttcc
     2401 ggggattatc tccgcgtaac tgacaacaag gggaaaacgc atgatgtgct gaccggaagt
     2461 gatgacggtc gccacagcaa cacgtctctg gcgtgggggg ctggcgtgca gtt
//
LOCUS       DAZFZC010000257         2469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFZC010000257 DAZFZC010000000
VERSION     DAZFZC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.263"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..904)
                     /locus_tag="ACHKXP_005241"
     CDS             complement(<1..904)
                     /locus_tag="ACHKXP_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO7650017.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLS"
     gene            1240..2442
                     /gene="nupC"
                     /locus_tag="ACHKXP_005242"
     CDS             1240..2442
                     /gene="nupC"
                     /locus_tag="ACHKXP_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO7650018.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT          618 a          637 c          574 g          640 t
ORIGIN      
        1 aagacagccc cgcagcaacc aggcttaatc caaagaccgt tgccgcagcg tggcttaaca
       61 gcggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga ccggaaaagt
      121 ggaacgccgc cgcagctgta gccatcatcg ccagattgac aaaaccggca atagtcatgg
      181 cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat
      241 gctgagtgag cgaggagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc
      301 ctgctgccag gaagaccgct tccgaagtag gtaaactcgg gatcaccatt cctttaccca
      361 gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca
      421 acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa
      481 tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga
      541 gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt
      601 ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcgcga atctgctccg
      661 ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca
      721 tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa
      781 tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca
      841 ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg
      901 tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgaca aacgagaatg
      961 attatcaaat tcatttaaat ggattgtggt gatttctctg atagaccagg attatgacta
     1021 cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact
     1081 ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt
     1141 cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat
     1201 gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt
     1261 gtactggcac ttgccgttgt tgcgattctc gcactgctgg taagcagcga ccgcaaaaaa
     1321 attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc
     1381 ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg
     1441 ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg
     1501 gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt
     1561 ctccagcaca ttcgcgtgtt gccggtgatc atccgcgcaa ttggtttcct gctctccaaa
     1621 gtcaacggca tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag
     1681 tctgaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac
     1741 accatggctg ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc
     1801 atgctggaac cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc
     1861 gtgctgtcgc tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc
     1921 aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc
     1981 aaagttgcca ttatcgttgc cgcgatgctg attggcttta tcgccctgat cgccgcgctg
     2041 aacgcactgt ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc
     2101 ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg
     2161 caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat
     2221 ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttcctg
     2281 gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat
     2341 gaagagcaag gtaacgtggt ttctcgcttc ggtctgaagc tggtttacgg ctctaccctg
     2401 gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aagaccatac ataaaaaagc
     2461 cggggataa
//
LOCUS       DAZFZC010000258         2399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFZC010000258 DAZFZC010000000
VERSION     DAZFZC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.264"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..972)
                     /locus_tag="ACHKXP_005243"
     CDS             complement(<1..972)
                     /locus_tag="ACHKXP_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO7650019.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLVGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQNYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGNIASSF"
     gene            complement(998..1690)
                     /gene="rfaY"
                     /locus_tag="ACHKXP_005244"
     CDS             complement(998..1690)
                     /gene="rfaY"
                     /locus_tag="ACHKXP_005244"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO7650020.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(1700..>2399)
                     /locus_tag="ACHKXP_005245"
     CDS             complement(1700..>2399)
                     /locus_tag="ACHKXP_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO7650021.1"
                     /translation="WSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFK
                     DEFAAVIPDNDSTQAACAKRLNIPEMNGCYFNAGVIYVNLKKWHEANLTPYLLKLLRG
                     ETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVL
                     IHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEY
                     IKGITSLIKYKLKK"
BASE COUNT          748 a          479 c          409 g          763 t
ORIGIN      
        1 aaatgaagat gcaatattac catttctttt caaatgtctg gatttcactt gatactgctt
       61 ttcattcgtg gctggaataa acgccacatc attccagcaa gaagcctgat aagcctcatt
      121 aaaatagttc tggcagggat aattcgtcca gtcgttccag ggcttcgtta cgccaacata
      181 atgaatcagc ttagtatcat cggtaatggt ctttttataa tcttcatcgc ttttattttt
      241 taactcataa tcaatgccat aaaagcaatc ataatcgccg ctaataaaaa tacaatgacc
      301 aaagaacaga atatttaaaa tatcctgatc aaggcattta tacttcgtgt gctgcgggtc
      361 acttgccaac atagcaatgg ctttattcat aaaatcattt ttcgcccatg cttcaagctg
      421 cagatatact acaccggcgt tgaaataatt ccctggaagt ccctctatag ccagacgctt
      481 cgctggtttt tcctgcataa aaggcacatc tttaataaca gctgcgaact tatccccatc
      541 aaaattaata tcaagcaatc caactaatga gcctttacag atgacatcag catcgatata
      601 taacagattt tcaatagttt cactcaaata ctggaacgca actaaacgga agaatgttgc
      661 ataagaccac tgtttcaggc tggggaagat atcaataaag gcattggaaa ctttataaat
      721 tattacttca acgttagcat ttttgctggt aaaagcatta acacgttgta gataatcttc
      781 atcaatgtag tcggtaaaga gatgaaactt aaccgcaagg tccgggttat gcataacaac
      841 tgattgcata gatactgccg caccaaataa aaagttacgg tcaataccgt aagcgatatt
      901 taacacacga tcggtgttag caatttcccc agagacagcg acttcagtaa tactctcagc
      961 taataaatcc atgttggttc cgttttgact gtgtggttta cgctttgcct tttaattttt
     1021 taataaattt gcgtagtttg gttctgtaga ttactgaata gtagccataa tcttttattt
     1081 cgttggcaat acccaggtga cgttccatgg ccagacgatc acgagcttta cgctctgccg
     1141 tacagctttt accggatagg tcaattatcc tgacaccatc tttactaacg ataaagttac
     1201 cccggtgtgg gtcgcccgat agcatattta aggcatgcaa tttttccatt gaagccttaa
     1261 tttctgcttt aacattttct ggaataatcg gcatatcatt gagctctacg ccctcgacat
     1321 actcgataag catgataaaa acactggcat agttgaaaat tttacgctca gccagaaaat
     1381 aaaaatcatt aggaaaagta agacctgcac ttctgacacg atcggtttca acgatcaaat
     1441 tctgatagta gtcacctttt acaaaagatt taaaaaaacg ttcattcctt ttcgttttcg
     1501 gagcaaagac tttaaaaaca tagcgaccat atttcgtatc aatcaacgat actttggtat
     1561 catcaatatt acgtaatact tttactgttt taaaattata ggccagtatt tcatcaaaga
     1621 tttctttata tttattatca ttatctttgt aataaaatga gaagccgcga taacgtatac
     1681 ttgtaatcat aagagtaatt tatttcttaa gcttgtactt aattaatgaa gttatgcctt
     1741 ttatatactc accatgggca aacagatgct tatattgttt ctgcatttct gcaacagtcc
     1801 gcgcctcttt aagaggatat ttcttccagg gagattgttc acgcgcgata ttaaagtatg
     1861 atgcagacgg atatccagcc cagctatgcc atggtttagt tatccctgta tagtgaatca
     1921 acactgtttt atcggtaatg gtttgctgat actttcggtg actacgatca taaagttcgt
     1981 ttttcagggt ataaatagta tcaaaatcct tcgcaaggta gatattattc atattaaatg
     2041 cgatattcaa cgcatcctga tctaaatatt taagagagcc atatttagtt tcccctcgta
     2101 aaagtttgag taaatacggt gtcaaatttg cttcatgcca ttttttaaga ttgacataga
     2161 taacgcctgc attgaaataa catccattca tttcgggaat gttgaggcgt tttgcacatg
     2221 ccgcctgagt actatcattg tcaggaatga ccgcagcaaa ctcatcttta aatataattt
     2281 ctgtgagagg tttcaggctt cctttacaaa caacatcggc atccagatac agcagtgtgg
     2341 aaatactttc actcagatat tcaaaagaca ataccctgaa gtatgtcgcg tacgaccaa
//
LOCUS       DAZFZC010000259         2390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFZC010000259 DAZFZC010000000
VERSION     DAZFZC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.265"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(96..1757)
                     /locus_tag="ACHKXP_005246"
     CDS             complement(96..1757)
                     /locus_tag="ACHKXP_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7650022.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPVYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILLRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPEGIVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(1754..2317)
                     /locus_tag="ACHKXP_005247"
     CDS             complement(1754..2317)
                     /locus_tag="ACHKXP_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7650023.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVELDEVPVYRQKKMQTEECHKEAPPAEKNSPVSLPPDDGQNLSPL
                     PGIDYMEDGLPDPVKAMGRILVENLIIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT          492 a          755 c          592 g          551 t
ORIGIN      
        1 gtcagcaggg aacaggcccg tttaagagtc acatcagcct cctgaaaata aaaaaaccgc
       61 cggagcggtt catgatggtt acagggtgaa cagggttata tgaaaaaaac cgcatattct
      121 ttctttttcg gttccgggtt gagggacatc agggagaccg cattgaacag cgccatcagc
      181 gggtcaattt ttccccgtcc actggcctgt ttggtgataa gaatggcgtt acctttaggc
      241 tccacccggg cattgccgac acaccaggcc atcaggggct ggtcaccgtg aatcagcacc
      301 ccttcagcca gtttgcgctc ggtggttttg atggccccac ccagtttcca gccctggctt
      361 atccccacca caattccttc agggatcccg gcttccgcca gtgaatccag aatctgcccc
      421 acccctgacg ggtcaatacc gatatggtcc agtaactcag cctcatgaat gcgacgcaca
      481 tattccgcca cttccgccgt gtcatccccg acacgccgga caatggtcat atctccacag
      541 gcaacaagat cctgaaaccg ggacgcctcg ctcttccgtc ggaccaccgc ggtttcatgc
      601 gcccaggcat ggccccagcc cagccattcg cgggtctccc ggtcacgccc aatcacatac
      661 atccccagca gatcatccag ccctccgccg tcaatcccca ctgtcaccac atcagcacgc
      721 agcaggatat cgtccaggct gatacaacgg ccctgctctt cccagaaatc cgcccccgcc
      781 cagcggtcag agcgcagggc aagaccaatt tccacattgg cgtgttttga catgaagccc
      841 cggaatgtct cttcaccggc ttccctggct tttcgatact cccggtacag aaaggcctca
      901 tccactgaat agccgagatt cggattgacc atggcgaggt tttccatcag caggtgagcc
      961 ccgctttcca ccatttcagg agggtgttca aagatcaccg gcagaaagtg tggatcatga
     1021 attttgccgt cgcgcacatc ccgggcgtac tgcagtttct gtctgaacac cccggcggga
     1081 ggttcattcg actgggtggt cgtatacacc acaaaccctt ccgggcggga ggcaaggcca
     1141 ccgatggctt cacgtaacat gtcctccgcc ttgtactgct tgccaaacag ccacagctca
     1201 tcaatcagcg tacccacgga cttgataccg gacaccgtat tcggatcggc tgccaccacc
     1261 ttcagggtgg tgtctgtcac ccggtgggtg atggtccgga tatgtgtctg cacctgacag
     1321 aggtcatcca gatcatcgtc ccgtcgtacc atatccctgg cagggttgaa ggcgttggct
     1381 gccacctcca ctgtcggggc cagaatggtg tagcccgccg cctgccgcca gttcagtaac
     1441 agtgcagtca tcatgatccc tgcggccagc gtggacttcg agtttttctt ggggataagg
     1501 ataaaaactt ccttgatatg gcgtacacca gtctgcgcat cgtaggagcc aaacagggcc
     1561 gccaccaggt caaacaccca cggtgcacag gactccccga acgtcgggct acccggtgca
     1621 tccacaatcc gcagttgttt aaaaatcgcc agtgcatgtg cagcctggtc cgggtaaacc
     1681 ggagccggaa taatcgacag cccctttttc aggcgctctg cccagtccgg gcaggcagtg
     1741 ctccatacag gtatcatccg ttgccctcat tatcgttatt caccaccagt cggggtggcg
     1801 gtggcaccgc aaaacggtta gccgcttttt tcgcggcatc accttttgcc gattttttcc
     1861 cggtatcccc ttttttatgg tgcgtgaact gcgccagtcg ccaggccgca tccagtgcca
     1921 gtttcggatc aattatcagg ttttccacaa ggatcctccc cattgctttt accggatctg
     1981 gaagaccgtc ttccatgtaa tcaataccgg gcagtggtga gaggttttgc ccgtcatcag
     2041 gcggcagtga aaccggggag ttcttttcag ccggaggggc ttccttatgg cactcctccg
     2101 tctgcatctt tttctgccgg taaacaggaa cctcatccag ctccaccgtc tcgcattgtt
     2161 tacgggctat aaacgcgagc acttccggat cttttgccag ttgtgaacct ttaaccctgg
     2221 ccgtcttcgc cgaataaccg gcagcaatgg ctgacgccgt tttgtttttc ccggacatga
     2281 gcgccagcgc aaattttcgt ttttgcgtcg tcagcaccgt ctcctcccgg gttcagaact
     2341 cactcagccg gacatgcttc aggccgtttt atcccgcctt tcatgccccc
//
LOCUS       DAZFZC010000260         2384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFZC010000260 DAZFZC010000000
VERSION     DAZFZC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.266"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..525)
                     /locus_tag="ACHKXP_005248"
                     /pseudo
     CDS             complement(1..525)
                     /locus_tag="ACHKXP_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(534..1301)
                     /gene="sctT"
                     /locus_tag="ACHKXP_005249"
     CDS             complement(534..1301)
                     /gene="sctT"
                     /locus_tag="ACHKXP_005249"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO7650024.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1303..1563)
                     /locus_tag="ACHKXP_005250"
     CDS             complement(1303..1563)
                     /locus_tag="ACHKXP_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7650025.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1573..2238)
                     /locus_tag="ACHKXP_005251"
     CDS             complement(1573..2238)
                     /locus_tag="ACHKXP_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7650026.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVNFFEKIQKVNSSEDNEEIFDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2228..>2384)
                     /locus_tag="ACHKXP_005252"
     CDS             complement(2228..>2384)
                     /locus_tag="ACHKXP_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO7650027.1"
                     /translation="AKRIISLLSESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWL
                     SGHNNVK"
BASE COUNT          834 a          485 c          380 g          685 t
ORIGIN      
        1 ctccccaatc tgataataaa gatatgatat ctccatcaag agttttaaaa agcagtgatt
       61 tatgattact ccaaaatact ttgttcgcca atgcaaaaaa aataatataa agaattgctt
      121 taacaaattc ttttacggtt tttaagccaa atatacgctt taagccattt actggattca
      181 atgaatcaaa cttaaattta acagcttcag ttgctaattg cattttactt tgaaaccagg
      241 agacaagtat tgttgtgaac atgcaaacca gcaaaaaagg aatgatcgtt ttcaaccaac
      301 caacccccat agctttaaaa tagtcccaaa tatctggttt agcgttatga tcaaggatgt
      361 attcaagaat cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca
      421 caagcattat tacagagatg gttaagtctc tactttttag aatttgccct tttttagaag
      481 catcctggag tttctttgtg ttggcttttc agttttattg gccatgaaac actctatttc
      541 tcaatgtata gttgcaattg ttctgggaaa aaggataacg gcattactcg ttcagccagt
      601 attgggccaa aataaataat taatattaaa aatgccagcg cacttttgac agtcagtgaa
      661 atggcaaaag catttagttg tgaagcataa cgtgccagca atcccaggac agcctcaccg
      721 cccagcataa cggcaatcac gggacttgcg taaacgatag tatgagtcat tatgttatta
      781 atatatgaga ataaaggctc taatttagga aaattaaaat tgcctgaagg ccataaatta
      841 taactttgcc aaagagtttc cagaataaag ttcaatccac cgttagtaag ataaaccgct
      901 gcagagaata ggttaaataa tcttgctaat tccgaagtat cgacacctgt agcaggatcc
      961 aaagtactac ttaacgttgc accacgttgg ttatcgataa aactacctat agcaaggaaa
     1021 atccaaaacg gggatgataa taagcaagca atgaacagcc ctatgataat ttctctggca
     1081 gcaaacaaag cgatatcgat ttcatttaag gtcgataatt catagtggta atgctgaact
     1141 aatgcacatg ataccactat tattattgga tgacggacta ctgttggtat attcccactg
     1201 gcgaaaaatg gtaacaaata gaaagttggt gccaatcggc aaaaaccgag cgctgtagca
     1261 gccagcagac tatgcaactg atagagtatc gcctccccca tcctatcctc tcgcgaatgc
     1321 taaattcagc atttcgatac caaacgaata taatttttca ccataccatc cgctcattaa
     1381 aaaaaagcaa atacttacgc ataataattt gacgccaaat ggcaacgtct gttcttgtaa
     1441 ctgtgttacc gtttgaaaaa gccctaccaa taaaccaaca aatgtcgcaa ctgcaatagg
     1501 tcctgcagac ataactaaaa tcagatacag cgccctattc cccgcaaaaa caatatcgtc
     1561 catatttttt tctcagggat taatggatag gtcaaaatat tgtaaaatga gtccttttga
     1621 taacattgtc caaccgtcca tcgcgacaaa caaaattaat tttatcggtg tagatatggt
     1681 taccggactc atcatcatca tacctaacgt taacagaacg ctggatataa ccagatccac
     1741 tacaacaaag ggtaaataga tataaaaacc tataataaat gcacttttta tctcactcaa
     1801 cgcatatgcc gggagtaacg agaaaattga aatattatca tcatcgaata tctcttcatt
     1861 gtcttcagag ctattcacct tctgaatctt ttcaaaaaag ttaaccaact ccggttcgga
     1921 atattttatc aaatatgatt tataaccact catgcctgtc tcaacaaaat taacaaccga
     1981 cgctacatta ttgaatgaaa gattctcatt ctgactgtta taatatattt ccttcccaac
     2041 cggcatcatt acaaacattg acaatagcaa agcaacccca ttaagtgtca tatttgatgg
     2101 cacttgctga agccccagcg cgtttcggac aataacaaaa acaatcgaga atttaataaa
     2161 atatgtcccg gatgctatta taaacggcaa tagtgtaaat aatgatagta tagctatcag
     2221 cgaaatgcta tttgacatta ttatgccctg ataaccaaga atgaatctct acgcccaatt
     2281 tatcatccac ttcaacaagt tctccatagc cagttagcgc gccatttaca cgtatttcta
     2341 tatttttctc agattcagaa agcagagata ttattctttt tgca
//
LOCUS       DAZFZC010000261         2343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFZC010000261 DAZFZC010000000
VERSION     DAZFZC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.267"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..714)
                     /gene="nleH1-1"
                     /locus_tag="ACHKXP_005253"
     CDS             complement(<1..714)
                     /gene="nleH1-1"
                     /locus_tag="ACHKXP_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-1"
                     /protein_id="HFO7650028.1"
                     /translation="MLSPYSVNLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDNGAQV
                     KVGNRTYRVVATDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNASPVSAAM
                     ERGIVHSNRPDLPPVDYAPPELPSVDYNRLSVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTSEVRCFNQYYGAGSAEKIYGNNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFV"
     gene            complement(883..1038)
                     /locus_tag="ACHKXP_005254"
                     /pseudo
     CDS             complement(883..1038)
                     /locus_tag="ACHKXP_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1316..1420
                     /locus_tag="ACHKXP_005255"
                     /pseudo
     CDS             1316..1420
                     /locus_tag="ACHKXP_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(1540..>2343)
                     /gene="nleC"
                     /locus_tag="ACHKXP_005256"
     CDS             complement(1540..>2343)
                     /gene="nleC"
                     /locus_tag="ACHKXP_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO7650029.1"
                     /translation="RSRLIDQHTVDMIGNTVLDALSRSQTFRDAVSYGIHNEKVHIGC
                     IKYRNEYELNEESSVKIDDIQSLTCNELYEYDVGQEPIFPICEAGENDNEEPYVSFSV
                     APDTDSYEMPSWQEGLIHEIIHHVTGSSDPSGDSNIELGPTEILARRVAQELGWSVPD
                     FKGYAEPEREAHLRLRNLNALRQAAMRHEENERAFFERLGTISDRYEASPDFTEYSAV
                     SNIGYGFIQQHDFPGLAINDNLQDANQIQLYHGAPYIFTFGDVDKHNQR"
BASE COUNT          683 a          547 c          402 g          711 t
ORIGIN      
        1 gacaaaaaga attccttttt gctccagtct atcaaacata tcgtaaatag catgctcagc
       61 ctgtgctggc aaggacgaaa tatttaaaag cgattctcca tttattttat ccattctaat
      121 accaataata tcaccattat tgccatatat tttttctgca ctcccggcac cataatattg
      181 gttgaagcaa cgaacttcgc ttgtcacctc ttcattgctt tgagatgtag taaacatctt
      241 caggactttt gttgcatcct cagcatcttc atatactaca gcgttccccc ctttgccaat
      301 aacattacca ggtactgaca acctgttata gtccacactc ggtaactctg gcggtgcata
      361 atcaacagga ggtaaatcag gtctgttcga atgaacaatg cctctttcca tagcagcgct
      421 cactggtgat gcattcagca tgacctcaat tttcctgcta atctccccct taggccatcc
      481 cagcctgtgc aacagattag taaaacaacc actatgactt tctcttgtaa cgcaaaactt
      541 attatcggtg gcaacaacac gatatgttct gttgccaacc tttacttgcg ccccattatc
      601 agaatgaacg gcagcatccc ttacagagga taaaacacga ttatcaggcg aagtcaggtt
      661 tctggttaaa gaattccatg aacatcccaa atttacagaa tatggcgata acatacattt
      721 caaccttcaa aataaaccca tctaaatttc tttaacaaac atccccctaa catggcaaca
      781 aaaattagaa atgaactctt gctttttgta tctggcaact aattcatcct tccagcatcc
      841 catcaaacga acgtttacga actataatgc aggtattacc ggtcactcga tatctggaga
      901 tacagacgta tcaacatgat tgagcggcat acgatactgc ttccatgccg ccagtaacga
      961 tgtctgctca tccgtcgcaa tatctaaatc tgcagcatcc tgaagtggtg cgatatactc
     1021 actgattaca agtaccagac cgatagatta aaaaaaacac aataacacgc tgcatttaaa
     1081 acaaaaattt taaatatgat ttaatacaag ctttaagcct ttaattatca gttttattta
     1141 ttttccagca attgattaat caattgttta tatacaaatt attccttttc tttctctcac
     1201 gcaaaattca caaaatattg ttgctgcaat ggacctgtac aatagtgaat cacggtactt
     1261 ttctgtgcac aacgtactgc agtttcttta cgatttatag ctgtactgtt gaattatgta
     1321 caatttgaat ccatgcatga cgtgtgatgc ctattgtgca tttttccgag cctcttttta
     1381 ctgggccgaa gccgatgatg ctgatagtct cgttccggcc atgttcactt actacagtaa
     1441 aattagggga attgccttta catacaatgt tgcacgatta cccatcatca actccgttaa
     1501 aatgtatgaa tagtaacctt atgtcactgc aaagacgaat catcgctgat tgtgtttgtc
     1561 cacatcccca aatgtaaaaa tataaggggc gccatgatac agttggatct gatttgcatc
     1621 ctgtaaatta tcgttgatag ccaatccagg aaaatcatgt tgctggataa atccgtatcc
     1681 tatgttagac acagcggaat actctgtgaa atcaggactc gcctcatatc ggtcactgat
     1741 cgtacccagc ctttcgaaga aagccctctc attctcttca tgcctcatgg cagcctgtcg
     1801 aagggcattt aggttacgta acctaagatg agcttcacgt tctggctctg catatccttt
     1861 gaagtcggga acactccatc ccagttcttg agcgacacga cgtgcgagaa tctcggtggg
     1921 tcctaactct atattactat ctccagatgg atcgctagat ccagtaacat gatgaataat
     1981 ctcgtgaatc agtccttcct gccacgatgg catctcataa gagtcagtat ctggcgcaac
     2041 actaaaactg acataaggct cttcgttatc gttttctcct gcttcgcaaa tggggaaaat
     2101 tggctcttgc ccgacatcat attcatataa ttcgttacag gttagtgatt gaatatcatc
     2161 aattttgaca gaagattctt cgttaagctc gtattcgttt ctgtatttaa tgcaaccaat
     2221 gtgtaccttc tcattatgaa tgccatagct tacggcatca cgaaatgtct gtgatcggct
     2281 caaagcatca agtacagtgt tgccgatcat atcgactgta tgctgatcga taagcctgct
     2341 acg
//
LOCUS       DAZFZC010000262         2336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFZC010000262 DAZFZC010000000
VERSION     DAZFZC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.268"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2336
                     /gene="rhsD"
                     /locus_tag="ACHKXP_005257"
     CDS             <1..>2336
                     /gene="rhsD"
                     /locus_tag="ACHKXP_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO7650030.1"
                     /translation="GELLAVYDRSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDD
                     TGRVVEQLNPAGLSYRYLYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVT
                     RSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVY
                     PDGLESRREYDEPGRLVSETSRSGDVIRYAYDNPHSELPATTTDATGSTRQMTWSRYG
                     QLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYE
                     YNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSH
                     SVFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVILWYYDESDRITHRTVN
                     GEPAEQWQYDGHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQH
                     ETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRS
                     FGSRAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYG
                     YSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYV
                     YHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLY
                     DPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRIQTVYQPGS
                     FAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVV"
BASE COUNT          541 a          676 c          800 g          319 t
ORIGIN      
        1 cggtgaactg ctggcggtat atgaccgcag caatacgcag gtgcgcgctt tcacgtatga
       61 cgcgcagcac ccgggccgga tggtggcgca ccgttacgcg ggaaggccgg agatgcgcta
      121 ccgctacgac gatacggggc gggtggtgga gcaactgaac ccggcagggt taagctaccg
      181 ctatctttat gagcaggacc gcatcaccgt caccgacagc ctgaaccggc gtgaggtgct
      241 gcatacagaa ggcggggccg ggctgaaacg ggtggtgaaa aaagaactgg cggacggcag
      301 cgtcacgcgc agcgggtatg acgcggcagg aaggctcacg gcgcagacgg acgcggcggg
      361 acggaggaca gagtacggtc tgaatgtggt gtccggcgat atcacggaca tcaccacacc
      421 ggacgggcgg gagacgaaat tttactataa cgacgggaac cagctgacgg cggtggtgta
      481 cccggacggg ctggagagcc gccgggaata tgatgaaccg ggcaggctgg tatcggagac
      541 atcgcgcagc ggggacgtca tccggtatgc ttatgataat ccgcacagtg aattaccggc
      601 cacgacaaca gatgcgacgg gcagcacccg gcagatgacg tggagccgct acggtcagtt
      661 gctggcgttc accgactgct cgggctacca gacccgttat gaatacgacc gcttcggcca
      721 gatgacggcg gtccaccgcg aggaaggcat cagcctttac cgccgctatg acaaccgtgg
      781 ccggttaacc tcggtgaaag acgcacaggg ccgtgaaacg cggtatgaat acaacgccgc
      841 aggcgacctg actgccgtta tcaccccgga cggcaaccgg agcgagacac agtacgatgc
      901 gtggggaaag gcggtcagca ccacgcaggg cgggctgacg cgcagtatgg agtacgatgc
      961 tgccggacgt gtcatcagcc tgaccaacga gaacggcagc cacagcgtct tcagttacga
     1021 tgcgctggac cggctggtac agcagggcgg ctttgacggg cggacgcaac gttatcatta
     1081 tgacctgacc ggaaaactca cacagagtga ggatgaggga cttgtcatcc tctggtacta
     1141 cgatgaatcg gaccgtatca ctcaccgcac ggtgaacggc gaaccggcag agcagtggca
     1201 gtatgatggc cacggctggc tgacagaaat cagccacctg agcgaaggcc accgtgtcgc
     1261 tgtccactac ggctacgacg ataagggccg cctgacgggc gaacggcaga cggtggagaa
     1321 cccggagacg ggggaactgc tgtggcagca tgagacgaaa cacgcctaca acgaacaggg
     1381 gctggcaaac cgcgtcacgc cggacagcct gccgccggtg gagtggctga cctacggcag
     1441 cggttacctg gcgggcatga agctgggcgg gacgccgctg gtcgagtata cgcgggacag
     1501 gctgcaccgt gagacggtcc gcagcttcgg cagcagggca ggcagtaatg ccgcatatga
     1561 actgaccagc acatacaccc ccgcaggcca gttacagagc cagcacctga acagcctggt
     1621 gtatgaccgt gactacgggt ggaatgacaa cggcgacctg gtgcgcatca gcggcccgcg
     1681 acagacgcgg gaatacggct acagtgccac gggcaggctg gagagcgtgc gcaccctcgc
     1741 accggacctg gacatccgca tcccgtatgc caccgacccg gcgggaaacc gcctgccgga
     1801 cccggagcta cacccggaca gcacgctcac ggcgtggccg gataaccgca tcgcagagga
     1861 tgcgcactat gtttaccact atgatgaata cggcagactg acggagaaga cggaccgcat
     1921 cccgacgggt gtgatacgga cggacgacga gcggacccac cactaccact acgacagcca
     1981 gcaccgcctg gtgttccaca cgcggataca gcatggcgag ccactggtcg agagccgcta
     2041 cctctacgac ccgctgggac ggcgaatggc gaaacgggta tggcggcggg agcgtgacct
     2101 gacggggtgg atgtcgctgt cgcgtaaacc ggaggagaca tggtacggct gggacggcga
     2161 caggctgacg acggtacaga ccgacaccac gcgtatccag acggtatacc agccggggag
     2221 cttcgcgccg ctcatccgca tcgaaacaga caacggtgag cgggagaaag cgcagcgccg
     2281 cagcctggcg gagaagctgc agcaggaagg gagcgaggac gggcacggcg tggtat
//
LOCUS       DAZFZC010000263         2289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFZC010000263 DAZFZC010000000
VERSION     DAZFZC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.269"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2289
                     /locus_tag="ACHKXP_005258"
     CDS             <1..>2289
                     /locus_tag="ACHKXP_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7650031.1"
                     /translation="TLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAAAA
                     AREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQASQS
                     SDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLK
                     ISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAM
                     ADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASGVT
                     KTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALA
                     EAQFRGTQIATARARAAVYCAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAA
                     QNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTID
                     EIAHNTPSMSLPEASDNEGRTRAALTEQKRLIDEQSSRVKSLQEQIAGYQYVLANPGW
                     TTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRRVA
                     LIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDK
                     QQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRN
                     NEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKTLT
                     EMQKQELLRNAALIDQQKIREQ"
BASE COUNT          547 a          548 c          757 g          437 t
ORIGIN      
        1 gacgttacgt gaactgatta tcaaaatttc ggcaaattcg cagtcattcc agtcggagat
       61 ccagcgggct tcccgtatgg gcagtgaata ttaccggacc ctgcagaatg gcgggcgtca
      121 ggccgctgcg gcagcccggg agcagcgacg tgccctggca gaactgaaca gccagttgac
      181 ggaaattcgc ggttctgctg tcggaatggc tggcgcattt gccggtgcct ttgccaccgg
      241 acacctgatt tcactggcgg atgagtggag ttccgtgaat gcccgtctga aacaggcgtc
      301 gcagtcatct gatgaatttt cgtcatcaca gaaagtgctg atggatatca gccagcggac
      361 aggcaccgca ttttcggata atgcggccct gtttgcccgc tcagccgcct caatgcgtga
      421 atatggttac agtgctgatg atgtgcttaa ggtgacggag gccatttcca cagggctgaa
      481 aatctccggt gccagtacgg cagaggcggg ctcggtgatc acccagttca gtcaggcgct
      541 ggcgcagggt gtattgcgtg gtgaggagtt taattcggtc aatgaaagtg gtgaccggat
      601 cgtacgtgca ctggctgcgg gtatgggcgt ggcccgtaaa gatctgaagg caatggcgga
      661 tgacggaaaa ctgacagcgg ataaagtggt ccccgcgtta atcagccagc tggggatatt
      721 acgtgatgaa tatgcggcca tgccggaaac ggtttccagt agtatcacga aggtggaaaa
      781 cgcctttatg gcctgggtgg gcggtgcgaa tgaggccagc ggggtgacga aaacgctctc
      841 cggcgtgctg aacggtgttg ccggacagat tgataatgtg gcaacagccg tgggggcact
      901 ggttgccgtc ggggttgccc ggtactttgg caatatggcc tccggagcga tgtctgccac
      961 ggcaggactt gtgactgctg cacgtaatga agttgcactg gcggaagcac agttcagggg
     1021 aacgcagatt gccacggcgc gggcaagagc agccgtgtac tgtgctcagc aggccgtggc
     1081 ggcagcccgc gggacggaga tgcagattgc agcagaggcc cgtctggcgg ccacacagga
     1141 acgcctgaac agaaatattg ctgccagaag cgccgcccag aatgcgctga acagtacaac
     1201 ggcggtgggc tcacgtctga tgagcggtgc gctggggctg gttggtggcg tacccggact
     1261 ggtgatgctg ggggctgcag catggtacac gctgtaccag aatcaggagc aggccaggga
     1321 gtctgcgcgc cagtatgcac tgacgataga tgaaatcgcg cataacacgc cgtcaatgtc
     1381 tttgcctgaa gcctcagata atgaaggacg aacacgggcg gcgctgacag agcagaagcg
     1441 gctgattgat gagcagtcca gtcgggtgaa atccctgcag gaacaaatcg ccgggtatca
     1501 gtatgttctg gcgaaccctg gctggacgac cggtgacgga ttcatgataa accatctgac
     1561 atcggtgaag accgtaacgg aagggcttgc tcaggcaaca gagcagcttg ccgttgagca
     1621 gtcccgtctg gcacagatgc aggaaaaagc gcagtccatt caggatgtgc ttgccgggct
     1681 ggaagaccgt cgtgtggcgt taattcgtca gcaggcggca gagcagaata aggtgtacca
     1741 gtccatgctg gttatgaacg gtcagcatac ggaattcaac cgtctgctgg ggctgggtaa
     1801 tgaactgctt cagcagcggc aggggctggt gaatgtgccg ttacggctgc cacaggccac
     1861 tctggatgat aaacagcaga gtgccctgac aaaaacagag cgtgagctgg ccctgtccag
     1921 actgaaaggg gaagaaaaag agcgtgtccg gctggggtat gcggcggatg acctcggttt
     1981 tgtgggtgat ccgtatcagg aggcgagaca acgttatatc agtaatgccc tggaagcctg
     2041 gcgcaataac gaggcgaata aacccaaatc ccggggtgga aaatcagaga cggaaaaagc
     2101 ggaagacagt ttttcccggc tgctgaagca gcagaaagag caactggcac tggtggggca
     2161 gaatacagag ctggcgaagc tgaaatacca gactgcgcag ggcgaactga aaaccctgac
     2221 ggagatgcag aagcaggagc tgctgcgtaa tgcggccctg attgaccagc aaaaaatccg
     2281 ggaacagtt
//
LOCUS       DAZFZC010000264         2268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFZC010000264 DAZFZC010000000
VERSION     DAZFZC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.270"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..353
                     /locus_tag="ACHKXP_005259"
     CDS             <1..353
                     /locus_tag="ACHKXP_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7650032.1"
                     /translation="VEELRQASETDLQPGTIEYERHRLTRAQADAQELKNARDSAEVV
                     ETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDE
                     LIPGLLSEYIEQSG"
     gene            328..2253
                     /locus_tag="ACHKXP_005260"
     CDS             328..2253
                     /locus_tag="ACHKXP_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7650033.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFS
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPGGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
BASE COUNT          563 a          570 c          663 g          472 t
ORIGIN      
        1 aagttgaaga actgcggcag gccagcgaga cagatctcca gccagggact attgagtacg
       61 aacgccatcg acttacgcgt gcgcaggccg acgcacagga gctgaaaaat gccagagact
      121 ccgctgaagt ggtggaaacc gcattctgta ctttcgtgct gtcgcggatc gcaggtgaaa
      181 ttgccagtat tctcgacggg atccccctgt cggtgcagcg gcgttttccg gaactggaaa
      241 accgacatgt tgatttcctg aaacgggata tcatcaaagc catgaacaaa gcagccgcgc
      301 tggatgaact gataccgggg ttgctgagtg aatatatcga acagtcaggt taacaggcta
      361 cggcattttg tccgcgccgg gcttcgctca ctgttcaggc cggagccaca gaccgccgtt
      421 gaatgggcgg atgctaatta ctatctcccg aaagaatccg cataccagga agggcgctgg
      481 gaaacactgc cctttcagcg ggccatcatg aatgcgatgg gcagcgacta catccgcgag
      541 gtgaatgtgg tgaagtctgc ccgtgtcggt tattccaaaa tgctgttggg tgtttatgcc
      601 tacttcatag agcataagca gcgcaacacc cttatctggt tgccgacgga tggtgatgcc
      661 gagaacttta tgaaaaccca cgttgagccg accatccgcg atattccgtc gctgctggcg
      721 ctggccccgt ggtatggcaa aaagcaccgg gataacacgc tcaccatgaa gcgtttcacc
      781 aatgggcgtg gtttctggtg cctgggcggt aaagcggcaa aaaactaccg tgaaaagtca
      841 gtggatgtgg cgggttatga tgaacttgct gcctttgatg atgatattga acaggaaggc
      901 tctccgacgt tcctgggtga caagcgtatt gaaggctcgg tctggccaaa gtccatccgt
      961 ggctccacgc ccaaagtgag aggcacctgt cagattgagc gtgcagccag tgaatccccg
     1021 cattttatgc gttttcatgt tgcctgcccg cactgcgggg aggaacagta ccttaaattt
     1081 ggcgacaaag agacgccgtt tggcctcaaa tggacgccgg atgatccctc cagcgtgttt
     1141 tatctctgcg agcataatgc ctgcgtcatc cgccagcagg agctggactt tactgatgcc
     1201 cgttatatct gcgaaaagac cgggatctgg acccgtgatg gcattctctg gttttcgtca
     1261 tccggtgaag agattgagcc gcctgacagt gtgacctttc acatctggac agcgtacagc
     1321 ccgttctcca cctgggtgca gattgtcaaa gactggatga aaacgaaagg ggatacggga
     1381 aaacgtaaaa ccttcgtgaa caccacgctc ggtgagacgt gggaagcgaa aatcggcgaa
     1441 cgtccggatg ctgaagtgat ggcagagcgg aaagagcatt attcagcgcc cgttcctgac
     1501 cgtgtggctt acctgaccgc cggtatcgac tcccagctgg accgctacga aatgcgcgta
     1561 tggggatggg gaccgggtga ggaaagctgg ctgattgacc ggcagattat tatgggccgc
     1621 cacgacgatg aacagacgct gctgcgtgtg gatgaggcca tcaataaaac ctatacccgc
     1681 cggaatggtg cagaaatgtc gatatcccgt atctgctggg atattggcgg gattgacccg
     1741 accatcgtgt atgaacgctc gaaaaaacat gggctgttcc gggtgatccc cattaaaggg
     1801 gcatccgtct acggaaagcc ggtggccagc atgccacgta agcgaaacaa aaacggggtt
     1861 taccttaccg aaatcggtac ggataccgcg aaagagcaga tttataaccg cttcacactg
     1921 acgccgggag gggatgaacc gcttcccggt gccgttcact tcccgaataa cccggatatt
     1981 tttgatctga ccgaagcgca gcaactgact gctgaagagc aggtcgaaaa atgggtggat
     2041 ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca atgaggcgct cgactgcttc
     2101 gtttatgcgc tggcggcgct gcgcatcagt atttcccgct ggcagctgga tctcagtgca
     2161 ctgctggcga gcctgcagga agaggatggt gcagcaacca acaagaaaac actggcagat
     2221 tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca ggaagaac
//
LOCUS       DAZFZC010000265         2232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFZC010000265 DAZFZC010000000
VERSION     DAZFZC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.271"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2232
                     /locus_tag="ACHKXP_005261"
     rRNA            <1..>2232
                     /locus_tag="ACHKXP_005261"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          570 a          487 c          705 g          470 t
ORIGIN      
        1 tctttatggg gtgactgcgt accttttgta taatgggtca gcgacttata ttctgtagca
       61 aggttaaccg aataggggag ccgaagggaa accgagtctt aactgggcgt taagttgcag
      121 ggtatagacc cgaaacccgg tgatctagcc atgggcaggt tgaaggttgg gtaacactaa
      181 ctggaggacc gaaccgacta atgttgaaaa attagcggat gacttgtggc tgggggtgaa
      241 aggccaatca aaccgggaga tagctggttc tccccgaaag ctatttaggt agcgcctcgt
      301 gaattcatct ccgggggtag agcactgttt cggcaagggg gtcatcccga cttaccaacc
      361 cgatgcaaac tgcgaatacc ggagaatgtt atcacgggag acacacggcg ggtgctaacg
      421 tccgtcgtga agagggaaac aacccagacc gccagctaag gtcccaaagt catggttaag
      481 tgggaaacga tgtgggaagg cccagacagc caggatgttg gcttagaagc agccatcatt
      541 taaagaaagc gtaatagctc actggtcgag tcggcctgcg cggaagatgt aacggggcta
      601 aaccatgcac cgaagctgcg gcagcgacgc ttatgcgttg ttgggtaggg gagcgttctg
      661 taagcctgtg aaggtgtgct gtgaggcatg ctggaggtat cagaagtgcg aatgctgaca
      721 taagtaacga taaagcgggt gaaaagcccg ctcgccggaa gaccaagggt tcctgtccaa
      781 cgttaatcgg ggcagggtga gtcgacccct aaggcgaggc cgaaaggcgt agtcgatggg
      841 aaacaggtta atattcctgt acttggtgtt actgcgaagg ggggacggag aaggctatgt
      901 tggccgggcg acggttgtcc cggtttaagc gtgtaggctg gttttccagg caaatccgga
      961 aaatcaaggc tgaggcgtga tgacgaggca ctacggtgct gaagcaacaa atgccctgct
     1021 tccaggaaaa gcctctaagc atcaggtaac atcaaatcgt accccaaacc gacacaggtg
     1081 gtcaggtaga gaataccaag gcgcttgaga gaactcgggt gaaggaacta ggcaaaatgg
     1141 tgccgtaact tcgggagaag gcacgctgat atgtaggtga ggtccctcgc ggatggagct
     1201 gaaatcagtc gaagatacca gctggctgca actgtttatt aaaaacacag cactgtgcaa
     1261 acacgaaagt ggacgtatac ggtgtgacgc ctgcccggtg ccggaaggtt aattgatggg
     1321 gttagcggta acgcgaagct cttgatcgaa gccccggtaa acggcggccg taactataac
     1381 ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cctgcacgaa tggcgtaatg
     1441 atggccaggc tgtctccacc cgagactcag tgaaattgaa ctcgctgtga agatgcagtg
     1501 tacccgcggc aagacggaaa gaccccgtga acctttacta tagcttgaca ctgaacattg
     1561 agccttgatg tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc tgcatggagc
     1621 cgaccttgaa ataccaccct ttaatgtttg atgttctaac gttgacccgt aatccgggtt
     1681 gcggacagtg tctggtgggt agtttgactg gggcggtctc ctcctaaaga gtaacggagg
     1741 agcacgaagg ttggctaatc ctggtcggac atcaggaggt tagtgcaatg gcataagcca
     1801 gcttgactgc gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg
     1861 ttctgaatgg aagggccatc gctcaacgga taaaaggtac tccggggata acaggctgat
     1921 accgcccaag agttcatatc gacggcggtg tttggcacct cgatgtcggc tcatcacatc
     1981 ctggggctga agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct
     2041 gggtttagaa cgtcgtgaga cagttcggtc cctatctgcc gtgggcgctg gagaactgag
     2101 gggggctgct cctagtacga gaggaccgga gtggacgcat cactggtgtt cgggttgtca
     2161 tgccaatggc actgcccggt agctaaatgc ggaagagata agtgctgaaa gcatctaagc
     2221 acgaaacttg cc
//
LOCUS       DAZFZC010000266         2230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFZC010000266 DAZFZC010000000
VERSION     DAZFZC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.272"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..222)
                     /locus_tag="ACHKXP_005262"
     CDS             complement(<1..222)
                     /locus_tag="ACHKXP_005262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650034.1"
                     /translation="MFFNLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHA"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="ACHKXP_005263"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="ACHKXP_005263"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO7650035.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRLIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTKH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTGTRNTYTSRFTEVAMAFASI"
BASE COUNT          570 a          548 c          660 g          452 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gttgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacgctt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agctggccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggctcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaaacac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacgggga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAZFZC010000267         2228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFZC010000267 DAZFZC010000000
VERSION     DAZFZC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.273"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..280
                     /locus_tag="ACHKXP_005264"
     CDS             <1..280
                     /locus_tag="ACHKXP_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7650036.1"
                     /translation="WLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAI
                     APSLSTSTQTRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            277..627
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005265"
     CDS             277..627
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7650037.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            658..>2228
                     /locus_tag="ACHKXP_005266"
     CDS             658..>2228
                     /locus_tag="ACHKXP_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFO7650038.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRV"
BASE COUNT          550 a          569 c          641 g          468 t
ORIGIN      
        1 atggctcagg ctctggcaga acgaagggcg tgtttcgcgg cgtcttccgg taacgacctc
       61 ttctgacact ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc
      121 tgtggcggcc attgcgccgt ctttatccac ttccactcag accagagtca gtgccagttc
      181 ctgcaaggtg gaattccgtc acggtaacat gacgctggaa aatccatcgc cagagctgct
      241 cacagtgttg atccgtgaac tgaccgggag gggaagatga tctcactccc atcaggtacc
      301 cgtatctggc tcgttgccgg cgttaccgat atgcgtaaat ccttcaacgg actgggagaa
      361 caggtacaac atgtgctgaa tgataatccc ttctccggtc acctgtttat cttccgtggc
      421 cgacggggtg acaccgtcaa aattctttgg gctgatgctg atggtctgtg cctgttcacc
      481 aaacgcctgg aggaaggcca gtttatctgg cctgcggtac gtgacggcaa ggtatccatt
      541 acccgctcgc aactggcaat gctcctcgat aagctggact ggcgtcagcc aaaaacatcc
      601 agccgtaact cactgacaat gttgtaaaaa actcctgacc gcattataaa aacggtcatg
      661 agtcagaaat acctcattcg catcgcagag ctggaaaggt tgctctctga gcaggctgaa
      721 gccctccgtc agaaagacca gcaactgagt ctggttgaag agacggaagc cttcctgcgc
      781 tctgcactga cacgtgccga agaaaagatc gaagaagatg aacgggaaat agaacatctg
      841 cgggctcaga tagaaaaact gcgccggatg ctgttcggta cccgttctga aaaactgcgt
      901 cgtgaagttg aactggctga ggctctgctg aaacaacgtg aacaggacag cgatcgttac
      961 agtgggcggg aagacgatcc tcaggttccc cgccagttgc gacagtcgcg ccatcgtcgt
     1021 ccgttaccgg cacaccttcc ccgtgaaata caccgcctgg agccagaaga aagctgttgc
     1081 ccggagtgtg gcggtgagct ggattatctg ggggaagtca gcgctgaaca gctggaactg
     1141 gtgagcagtg ccctgaaagt gatccgcaca gaacgggtaa aaaaagcctg tacaaaatgt
     1201 gactgtattg ttgaagcacc ggcgccgtcc cgcccgatag agcgtggtat cgcgggcccc
     1261 ggattacttg cccgcgtgtt aacgggaaaa tactgcgaac atctgccact gtatcgtcag
     1321 agtgaaatct ttgcccgcca gggtgtcgaa ctgagccggg ccttactctc caactgggtt
     1381 gacgcgtgct gccagttaat gacaccggtg aatgatgccc tgtaccgtta tgtaatgaac
     1441 acccgcaagg ttcacactga tgacacaccg gtaaaggtac tggcaccggg tcagaaaaag
     1501 gcgaaaacag ggcgtatctg gacgtatgtc cgggatgatc gcaatgtggg ttcgtcatct
     1561 cctccagcgg tctggttcgc gtactcgccg aaccggcagg ggaaacaccc ggagcaacac
     1621 ctccgcccct tccggggtat cctgcaggcg gatgcgttca caggttacga caggttgttc
     1681 agtgcagaac gtgaaggtgg tgcactgaca gaagttgcgt gctgggccca tgcccggcga
     1741 aaaatccacg atgtatacat cagcagcaaa agtgcgacgg cagaagaagc cctgaagcga
     1801 atcagtgaac tgtacgccat cgaggatgaa atacggggat taccggagtc agagcgtctt
     1861 gccgtcaggc agcagcgaag caaagtgtta ctgacgtcgc tgcatgaatg gatggtggag
     1921 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
     1981 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga taataatgct
     2041 gcggaaagag cgcttcgtgc agtctgtctc ggaaagaaaa actttatgtt ctttggcagc
     2101 gatcacggcg gcgagcgtgg agcactgttg tacgggctga tcggcacctg ccgtctgaac
     2161 ggtatcgatc cggaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
     2221 aaccgagt
//
LOCUS       DAZFZC010000268         2222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFZC010000268 DAZFZC010000000
VERSION     DAZFZC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.274"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            36..845
                     /gene="yqeI"
                     /locus_tag="ACHKXP_005267"
     CDS             36..845
                     /gene="yqeI"
                     /locus_tag="ACHKXP_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7650039.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            842..1323
                     /gene="yqeJ"
                     /locus_tag="ACHKXP_005268"
                     /pseudo
     CDS             842..1323
                     /gene="yqeJ"
                     /locus_tag="ACHKXP_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
     gene            complement(1356..1436)
                     /locus_tag="ACHKXP_005269"
     CDS             complement(1356..1436)
                     /locus_tag="ACHKXP_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650040.1"
                     /translation="MKDVDQIFDALDCHILREYLILLFYD"
     gene            complement(1472..1897)
                     /gene="yqeK"
                     /locus_tag="ACHKXP_005270"
     CDS             complement(1472..1897)
                     /gene="yqeK"
                     /locus_tag="ACHKXP_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO7650041.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLNSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            2091..>2222
                     /locus_tag="ACHKXP_005271"
     CDS             2091..>2222
                     /locus_tag="ACHKXP_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650042.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     "
BASE COUNT          780 a          394 c          380 g          668 t
ORIGIN      
        1 atctccattt cgattgattt agtgtttatt gacgtatgta ctggattatt aacgataata
       61 tcgagttctg gcctgagcac cgaaaattaa tatcggtaca taacgccgat cttaacgtcg
      121 ttctgacaac gccagccagt cgatgtttat cacttctact tgaagctttt cctgatgtgg
      181 ttgcacaaca agattttttc accagagtct gggaagaaga aggtatgcgt gtgcctacta
      241 acacgttata tcagaacata tccattatca gacgcggatt tcgcgctgtt ggtgatacta
      301 cccactcgct aattgcaacc gtgccgagaa gaggattcaa gatccataat gacattaaca
      361 tacaaaatca tgtaataaac tcgtcaacag acgcacatac acacaatgcc ccacctgcca
      421 taaaagttaa tgcgggatac aaagagagca ttggtggcgc aaagaatttc aataacaaaa
      481 tcctcaaaca tataaaaagt catctaatta tgttgagcgc atttgtcata ggcgcatatt
      541 ctgcatattg gctatggaat aataatcaac cgaagccatt tttcaaggat tacaagactg
      601 ttgcggaaat taatggctgc cattttaatg taacagaaga tacaattgat gggttgaaag
      661 agttcgataa atataaaaca cggatactgg attcggggat taactgtaaa aaacatccgt
      721 ggttatactt ccctcttgca aaatcctcac cggggatgat tgttatggca tgcaataaaa
      781 actataacca acatgaagtg gcagactgtt taaccctgtc ttaccgtgag gttaatcgtg
      841 attgattata aaaaaaaact tattatttat tttagttttt atcagtgggt ttattctctt
      901 cactgtctat tcttacactg cagaaaaaat gatatataac gaaacatgca ctgcaaattg
      961 ggtaattttt aatgaccagg gacgagcaaa tcttaccatc gactttatgt ataacaaaaa
     1021 aaaaataaaa caggaactgt tgcgttgagt ggaacctggc aacaaggtaa cagagaaagt
     1081 aaatcaatac ggcgaaatat tgaatacaca tggattgaaa actatgacac agcccattta
     1141 acatcaaaga aagttaataa gtttgagatt atggatcaag ttgatgatga tagacttgca
     1201 caacttattc ctgattttta tgtctttcca gaaaaaagtg taagctataa tatactaaag
     1261 caaggtaagc atgcttttat tttgagcatt ggtaacagag caataatgca ttgtgcaagg
     1321 taaactaaaa tagataacaa aaagatggaa cctcgttaat cataaaacaa taaaattaaa
     1381 tattctcgca gtatatggca gtctaaagca tcaaagattt gatcaacatc tttcatttta
     1441 gacatctcct tgcaatgtaa aatatcatgt atcaacaaca tctggtgcaa atccatgagt
     1501 cgaactcgac attcatctta gctcgattat tattccttcg tacagtcgat gtaaacaata
     1561 cagaaagagg attattaaga accagtttac tcagtgaatc aatagaggaa aggactaacg
     1621 tttctttaaa agaattgatt tcatcatctg ttaaactaaa ctcatcattg acagatcgtg
     1681 agatataact gtttttaact ttactcttta cgttgacttt attgacagaa ttaacattca
     1741 catatcttga atttaatgtc catgatgttg tttgcgtgag aacattctca gcattaagga
     1801 atttttttaa cggaatcctt ggtttctttt tcgagtccga atttacaata tcatgcatat
     1861 agaccatttc atgaatctgc gaaaattcaa tgtccattgt atacctcaca tttttaccgt
     1921 gactcgatgt tactgttcaa taatcacctt ccatcaatac taaaattaat acccctaatg
     1981 tgccgataac aaatatagtc attctacgta acgtctccat aaggtgatat ttgacattat
     2041 cagaagctgc gaattcggat tttgctctaa tcaaatgaga gaaatgtaaa atgaagccac
     2101 gaaatattaa taatagccta ccactgcaac cattagttcc tgatcaggag aacaaaaata
     2161 agaaaaatga agagaaatcc gttaatccag ttaaaatcac aatggggtct ggtttaaatt
     2221 at
//
LOCUS       DAZFZC010000269         2188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFZC010000269 DAZFZC010000000
VERSION     DAZFZC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.275"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1485
                     /locus_tag="ACHKXP_005272"
     CDS             <1..1485
                     /locus_tag="ACHKXP_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7650043.1"
                     /translation="AGNRLPDPELHPDSALSMWPDNRIARDAHYLYRYDRHGRLTEKT
                     DLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGRRVAKRVWR
                     RERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGE
                     QAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCG
                     LTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYDEWGNLLNEEN
                     PHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPV
                     QYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVF
                     KTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPP
                     GYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1482..2051
                     /gene="ybfC"
                     /locus_tag="ACHKXP_005273"
     CDS             1482..2051
                     /gene="ybfC"
                     /locus_tag="ACHKXP_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO7650044.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPAQLLAVLDNSIMVKYFKEEKPTYA
                     RMTAHLPNKDESYECLTKIQHELLRSEEK"
BASE COUNT          640 a          509 c          567 g          472 t
ORIGIN      
        1 gcaggtaacc gcctgcccga cccggagctg cacccggaca gcgccctcag catgtggccg
       61 gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccgtca cggcaggctg
      121 acggagaaaa ccgacctcat cccggaaggg gttatccgca cggatgatga gcgcacccac
      181 cggtaccatt acgacagtca gcaccggctg gtgcactaca cgcggacaca atatgaagag
      241 ccgctggtcg aaagccgcta tctttacgac ccgctgggcc gcagggtggc aaaacgggtg
      301 tggcgacgtg aacgggacct gacgggctgg atgtcgctgt cacggaaacc gcaagtgacc
      361 tggtacggct gggacggcga ccgcctgacc acaatacaga acgacagaac ccgcatccag
      421 acgatttatc agccggggag cttcacgcca ctcatcaggg ttgaaaccgc caccggtgag
      481 caggcgaaaa cgcagcgccg cagcctggcg gatacccttc agcagtccgg cggcgaagac
      541 ggtggcagtg tggtgttccc gccggtgctg gtgcagatgc tcgaccggct ggaaagtgaa
      601 atcctggctg accgggtgag tgaggaaagc cgccgctggc tggcatcgtg cggcctgacg
      661 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
      721 taccactgcg accatcgcgg cctgccgctg gcgcttgtca gcacggaagg ggcaacagaa
      781 tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      841 cagcagctta tccggctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      901 cgccaccgct attatgaccc gctgcagggg aggtatatca ctcaggatcc gattgggctg
      961 aaggggggat ggaattttta tcagtatccg ctgaatccgg ttcagtatat agattcaatg
     1021 ggactggcat caaaatatgg acacttaaat aatggcggat atggagcgag acccaacaaa
     1081 ccgcctacgc ccgatccaag taaattgccg gacatagcga aacaattaag actgccatat
     1141 cctattgacc aggccagtag tgcgcctaat gttttcaaaa cattcttcag agcattaagc
     1201 ccttacgact acacactgta ttgcaggaag tgggtaaaac caaatctgac ttgtacgcca
     1261 caggatgatt cccagtatcc agggatggat acaaagacag caagtgatta cctgccacag
     1321 acaaattggc caacaactca attaccacca ggatatactt gtgcagaacc ctatttattc
     1381 ccagacatta ataaacccga tgggccagca acagcaggga tagatgattt gggtgaaatt
     1441 ttagctaaga tgaaacagag aacatcgaga ggaataagaa aatgaaaaga gttttgttct
     1501 ttttgctgat gatatttgtt agttttggtg ttatagctga ttgcgaaata caagctaaag
     1561 atcatgattg ttttactatt ttcgctaagg gtactatttt ttccgcgttt cctgtcttaa
     1621 ataataaagc catgtggaga tggtatcaga atgaggacat cggtgagtat tattggcaaa
     1681 cagaactggg tacatgtaaa aataataaat tcacaccaag cggggcgaga ctcttaattc
     1741 gcgtcgggtc actacgcctt aatgaaaatc atgccatcaa aggaacgttg caggagttga
     1801 taaatacagc agaaaaaacg gcgtttctcg gcgatcggtt tagaagttat ataagagcgg
     1861 gtatctatca aaaaaagagc agtgatccag ctcagttgtt ggcagtcctt gataattcaa
     1921 tcatggttaa atattttaaa gaagagaagc caacttatgc tcgaatgaca gcacatctcc
     1981 cgaacaaaga tgagtcttac gaatgtttaa caaaaataca gcatgaactg cttcgtagtg
     2041 aagaaaaata aaacatcttc ctgagaaaat aaattaacca ttcggaaaaa catgttctta
     2101 atggatatcc aggtacacca gaaggtcgat ttggaaaaat ggattccgat ggtaagaata
     2161 tcatatattc tgaatgtaat gttttacc
//
LOCUS       DAZFZC010000270         2162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFZC010000270 DAZFZC010000000
VERSION     DAZFZC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.276"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            7..276
                     /locus_tag="ACHKXP_005274"
     CDS             7..276
                     /locus_tag="ACHKXP_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7650045.1"
                     /translation="MNILKKLMQRMCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRGDGTWENIGGMK"
     gene            complement(393..668)
                     /locus_tag="ACHKXP_005275"
     CDS             complement(393..668)
                     /locus_tag="ACHKXP_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO7650046.1"
                     /translation="MPFSIKNIFSNSKGSYPEISGPVQDKPVSKNCTLTSTTCSMNDY
                     TVFSRKSCTFDMRPPGAGDRTPQLKLSASELIWLSKTIDTERNNIKE"
     gene            complement(731..2092)
                     /gene="espK"
                     /locus_tag="ACHKXP_005276"
     CDS             complement(731..2092)
                     /gene="espK"
                     /locus_tag="ACHKXP_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO7650047.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSAGIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTILTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIIPEGIECLTVCRCHISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLQTLSLTGCSNIILPEILPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKSH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADEIVNSLKLIANKSDG
                     YGQSITASELRWIYRNKDNDQIMKNIKFYLRGKEIPAEKILGAPEWKDYKPKYSGATY
                     KYS"
BASE COUNT          516 a          408 c          507 g          731 t
ORIGIN      
        1 aatgacatga acatactaaa aaaacttatg cagcgtatgt gtggttgcgg aaagcatgat
       61 ggccgtgaac acgtgcagtc gcttacagca cagctgcgac tgggaccggc agacattctg
      121 gagtccgatg agaatggcat tatcccggag caggacaggg taatcacgca ggtggtgata
      181 ctggatgcgg ataaaaagca gatacagtgc gtggtaagac cgctgcaaat tctgcgtggt
      241 gacgggacgt gggaaaatat tggcgggatg aagtaactcg acagcttcac aaaaccggag
      301 tccggctcca ttttaccacc tccacgggag gtggttgctt gcaatcagat gtttcgggcc
      361 tgcggctttt tgtgttccct agtaagtggt tactattctt ttatgttatt cctctctgta
      421 tctattgtct tagatagcca tatgagttca gatgctgaga gttttaattg tggtgttctg
      481 tctcctgctc cgggtggacg catgtcaaaa gtgcaggatt ttctgctgaa tactgtgtag
      541 tcattcatag agcatgttgt tgaggttagt gtacaattct tcgatactgg cttgtcctgc
      601 acaggtccgg atatttctgg gtagcttcct tttgaatttg aaaatatgtt tttgattgaa
      661 aatggcatcg tgctttgtcc tttgaactac gatatattgt tttgtgttct ttcgttgtga
      721 gtaggtatca ttaagaatac ttatatgtgg ctccagagta tttgggtttg tagtctttcc
      781 actctggtgc gcctaatatt ttttctgctg gtatctcttt cccacgcaag taaaacttta
      841 tgtttttcat tatctggtcg ttgtctttgt tgcgataaat ccatcgtaat tcgctggcag
      901 taatactctg accataacca tctgacttat tagcaatgag tttgagtgag ttgacaatct
      961 cgtcagcaca aaaaataacc gttttgttgc gtgttaatgt ctgaaactcc aggccggcct
     1021 tgcttgtttt tgcccataat ttgtcgcgat tgtatacgga aaggtcggcc atggtgacat
     1081 tataattatt gtggctcttt aggtgttgtt taaattttat ccctctttcg tgttcattta
     1141 atgctctttt gatcagtgga tcttgcttat atttgcgagg tgttctgggg tcccatactg
     1201 cgtttgttaa cgtattctga gttataatat cttttttgga aaagtcgtta actagtttaa
     1261 tgctgttgac aagtttttct ctgggtgaag atagtccata gacaatgtct ccctcgcgaa
     1321 agtgtaaggc cacatccaga gcgttgccct gaaaggtgat gttttttgct ttaactacat
     1381 ctggagagag tagtacattt tgtaaatcaa gatccagccc atcaggcatc ccttcaacac
     1441 cgatgttcca cgtggttttc tgctccgcat gaatggttac cgatgtcaca ctctccggaa
     1501 gtatctccgg cagtataata ttgctacatc cagtcagcga tagcgtctgc agtgacggtg
     1561 atatcaggtt gtcaattctg gcctgattct ccgggttata gctattgatg ctgagagatg
     1621 ataacccgtt tggaacattt tttatgctgt ctgtggcgct accttttatc tcaaggtagc
     1681 ggacgctctc tggcactcca gatatatggc agcgacaaac cgtcaggcat tcgatgcctt
     1741 caggtatgat gtgtataaat ggtgaatcag ttaattcaat atgtagattt tttactgtcg
     1801 atggtatgtc tgtgatttgt aatttttcac agttaatgac ggagaggttt tgtgtattaa
     1861 ccggcaatcc tgtaaggatt gtcaggttgt tgcagttttc gattgtaact gatgttattt
     1921 cgtttggtaa ctgtggcaga tactcaatat cacaatcttt gatatataaa cgaccactgg
     1981 cccgcgcttc ttctatctga attggtgtga tttcgctttt gatgcctgct gatgtttcag
     2041 cggaatagga gcagaatgtc ccggtcggtc gtaattgcga tgtaggaagc attgataaat
     2101 tccattttta gaagtaaata acagagtaag ttctatccat actactaatc aacaagctaa
     2161 tt
//
LOCUS       DAZFZC010000271         2155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFZC010000271 DAZFZC010000000
VERSION     DAZFZC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.277"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..480
                     /locus_tag="ACHKXP_005277"
     CDS             <1..480
                     /locus_tag="ACHKXP_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO7650048.1"
                     /translation="SKQGDTKDTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQ
                     SGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLF
                     RWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWIYGVDL"
     gene            477..1178
                     /locus_tag="ACHKXP_005278"
     CDS             477..1178
                     /locus_tag="ACHKXP_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7650049.1"
                     /translation="MKNIAAQMVNFDREQMRRITNNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            1175..1465
                     /locus_tag="ACHKXP_005279"
     CDS             1175..1465
                     /locus_tag="ACHKXP_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7650050.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            1536..1814
                     /locus_tag="ACHKXP_005280"
     CDS             1536..1814
                     /locus_tag="ACHKXP_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650051.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            1947..>2155
                     /locus_tag="ACHKXP_005281"
     CDS             1947..>2155
                     /locus_tag="ACHKXP_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650052.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLD"
BASE COUNT          618 a          479 c          603 g          455 t
ORIGIN      
        1 tcaaaacagg gggacacaaa agacactatt acaaaagaaa aaagaaaaga ttattcgtcc
       61 gagaattctg gcgaatcctc tgaccagcca gaaaacgatc tttctgtggt taaaccggat
      121 gctgcaattc agagcggcag caagtgggga acagcagaag acctgaccgc cgcagagtgg
      181 atgtttgaca tggtgaagac catcgcacca tcagccagaa aaccgaattt tgcagggtgg
      241 gctaacgata tccgcctgat gcgtgaacgt gacggacgta accaccgcga catgtgcgtg
      301 ctgttccgct gggcatgcca ggacaacttc tggtccggta acgtgctaag tccggccaaa
      361 ctccgcgaca agtggaccca actcgaaatc aaccgtaaca agcaacaggc tggcgtgaca
      421 gctggaaaac caaaactcga cctgacaaac actgactgga tttacggggt ggatttatga
      481 aaaacatcgc cgcacagatg gttaactttg accgtgagca gatgcgtcgg atcaccaaca
      541 acatgccgga acagtacgac gaaaagccgc aggtacaaca ggtagcgcag atcatcaacg
      601 gtgtgttcag ccagttactg gcaactttcc cggcgagtct ggctaaccgg gaccagaacg
      661 aactgaatga aatccgccgc cagtgggttc tggctttccg ggaaaacggg atcacctcga
      721 tggaacaggt taacgcagga atgcgcgtag cccgtcggca gaatcgacca tttcttccat
      781 cacccgggca gtttgttgca tggtgccggg aagaagcatc cgttatcgcc ggactgccaa
      841 acgtcagcga gctggttgat atggtttacg agtattgccg gaagcgtggc ctgtatccgg
      901 atgcagagtc ttatccgtgg aaatcaaacg cgcactactg gctggttacc aacctgtacc
      961 agaacatgcg ggccaatgcg ctgactgacg cggaattacg gcgcaaggct gccgatgaac
     1021 tgacctgtat gacagcacga attaaccgtg gtgagacgat acctgaacca gtaaaacaac
     1081 ttcctgtcat gggcggcaga cctctaaatc gagcacaggc tctggcgaag atcgcagaaa
     1141 ttaaagctaa gttcggactg aaaggagcaa gtgtatgacg ggcaaagagg caattattca
     1201 ttacctcggg acgcataaga acttctgtgc acaggacgtt tccgcggtaa caggcgcaac
     1261 cgtaaccagc ataaatcagg ctgcggctaa aatggcgcgg gcaggaatcc tggtcattga
     1321 tggtaaggtc tggcgaacgg tgtattaccg gtttgctacc agggaagaac gggaaggaaa
     1381 ggtgagcacg aacctgattt ttaaggagtg tcgccagagt gcagcgatga aacgggtatt
     1441 ggcggtatat ggagttaaaa gatgaccatc tacatcactg agctaataac aggcctgctg
     1501 gtaatcgcag gcctttttat ttgggggaga gggaagtgaa cgatagctac cgacagtttg
     1561 aaaactggtg gtcaaaagac aaaagccagt tcacgggaga cgatgaatta aaagagtttg
     1621 cctgggtgat atggcaggca tcgcgctctg ctattgaact ggatatcgac tggcccgaat
     1681 cgaatgacga cctttggaaa gatggtgaag aaggtgctta tgcgatgggt tatgaggatg
     1741 ggcgtgacaa aacggtaatt gcagtaatga aagccatcag ggccgcagga atcaaagaaa
     1801 agaatttcga ttaagcaaat atcacttcaa taaatcgctt ttaaggcatc acaatcgctc
     1861 tgtggtgagg taagcacgtg caaggcatgc cgataagcag cgagaatgaa aaatgcgtca
     1921 gaatgcgttt gaggaggttt taagaaatga gtacgatagc tgagcttgtc agggctaatt
     1981 ttcgtgaaga gttggtgcgt tggtatcggt atcgttcatc gtccagtttg ccgcttgatg
     2041 agttgtatga gcattcacct gccgcacgac gctatccgcg tgaccgtgtt cttcgacggt
     2101 tgttcaaact caacaatgag tttcagcgca acagaattat ccggagtctg gattt
//
LOCUS       DAZFZC010000272         2143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFZC010000272 DAZFZC010000000
VERSION     DAZFZC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.278"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..240
                     /locus_tag="ACHKXP_005282"
     CDS             <1..240
                     /locus_tag="ACHKXP_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7650053.1"
                     /translation="AYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQ
                     IFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            237..917
                     /locus_tag="ACHKXP_005283"
     CDS             237..917
                     /locus_tag="ACHKXP_005283"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7650054.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEKVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            914..1072
                     /locus_tag="ACHKXP_005284"
     CDS             914..1072
                     /locus_tag="ACHKXP_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO7650055.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRESV"
     gene            1069..1821
                     /locus_tag="ACHKXP_005285"
     CDS             1069..1821
                     /locus_tag="ACHKXP_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650056.1"
                     /translation="MIHFHGGPITPDTCALKAWKGRHAFISFANPAQIDLASEVTQSF
                     ALDNGAFTFWTKNKAVDWNEYYRFVERWGNHPRFSFAVIPDVIGGTSEENDALIAAWP
                     HGKFIGAPVWHMNEPDERFIRLCHEFPRVCIGSMGEYDAKRPRACRAKLRDLIRHVVD
                     QYGYPITKIHGLRMLNKDIFTHVPLSSADSTNVARNIGIDKSWVGSPYAPASKETRTQ
                     VLVERIESFNSASSLNYNAERDVFTPQLAFEV"
     gene            1829..2044
                     /locus_tag="ACHKXP_005286"
     CDS             1829..2044
                     /locus_tag="ACHKXP_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7650057.1"
                     /translation="MTIEHNNALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
BASE COUNT          585 a          514 c          543 g          501 t
ORIGIN      
        1 gcatacactg cagaacgtca gccggaacgc gacatcactc cggttaacga tgaaaccatg
       61 caggagatta acactctgct gatcgccctg gataaaacat gggatgacga cttattgccg
      121 ctctgttccc agatatttcg ccgcgacatt cgcgcatcgt cagaactgac acaggccgaa
      181 gcagtgaaag ctcttggatt cctgaaacag aaagccactg agcagaaggt ggcagcatga
      241 caccggacat tatcctgcag cgtaccggga tcgacgtgag agctgtcgaa cagggggatg
      301 atgcatggca caaattacgg ctcggcgtca tcaccgcttc agaagttcac aacgtgatag
      361 caaagccccg ctcaggaaag aagtggcctg acatgaaaat gtcctacttc cacaccctgc
      421 tggctgaggt ttgcaccggt gtggctccgg aagttaatgc taaggcgctg gcctggggaa
      481 aacagtacga gaacgacgcc agagccctgt ttgagttcac ttccggcgtg aatgttactg
      541 aatccccgat catctatcgc gacgaaagta tgcgcaccgc ctgctctccc gatggtttat
      601 gcagtgacgg caacggcctt gaactgaaat gcccgtttac ctcccgggat ttcatgaagt
      661 tccggctcgg tggtttcgag gccataaagt cggcttacat ggcccaggtg cagtacagca
      721 tgtgggtgac gcgaaaagat gcctggtact ttgccaacta tgacccgcgc atgaagcgtg
      781 aaggcctgca ttatgtcgtg attgagcgga atgaaaagta catggcgaat tttgacgaga
      841 aggtgccgga gttcatcgaa aaaatggacg tggcactggc tgaaattggt tttgtatttg
      901 gggagcaatg gcgatgaagc atcctcacga taatatccgg gtaggcgcga tcactttcat
      961 ctactccgtt acaaagcgag gctgggtatt tcccggcctt tctgttatca gaaatccact
     1021 gaaagcacag cggctggctg aggagataaa taataaacgg gagagtgtat gattcatttt
     1081 cacggtggtc caataactcc cgatacctgt gcgttgaaag cctggaaagg cagacacgca
     1141 ttcatcagtt ttgctaatcc agctcagatt gatctggctt ccgaagtcac ccaatcattt
     1201 gctcttgata atggtgcatt cacattctgg acaaaaaaca aggccgtaga ctggaatgaa
     1261 tattacagat ttgttgaacg ctggggtaat caccctcgtt tctcattcgc ggttatcccg
     1321 gatgttatcg gcggaaccag tgaagagaat gacgccctga ttgcggcatg gcctcacggt
     1381 aaatttattg gtgctccggt gtggcacatg aacgaaccag atgagcgatt tattcgtctg
     1441 tgccatgagt ttccccgcgt ctgcatcggc tcgatggggg aatacgatgc aaaacgaccg
     1501 agagcgtgtc gggctaaact acgcgatctt atacgtcatg ttgtagatca gtacggctac
     1561 ccaatcacca agattcatgg gttacggatg ttaaataaag acatttttac tcatgtaccg
     1621 ctttcgtctg cagacagcac gaacgtcgca agaaatatag gcatcgacaa atcctgggta
     1681 ggttcgccat atgctcccgc aagtaaagaa acccgtacgc aagttcttgt ggaacgcatt
     1741 gaatcattca acagtgccag ttcacttaat tacaacgctg aacgggacgt ctttacccct
     1801 caactggcat tcgaagtgtg aggccaatat gacaatagaa cataataacg cccttcgcag
     1861 cattgcccgt caggctaatt ctgaaatcaa aaaagccaga cagcagtttc cggataaaaa
     1921 cgtcgatgac atttgccgta gcgtactaaa gaagcaccgc gaaacggtaa cgctgatggg
     1981 attcacaccg actcatttaa gcctggcgat cggcatgttg aacggcgtct ttaaggaacg
     2041 gtgaacatga aaagcaaaat catcagggag ctacaggctc cttttttatt attcgcattc
     2101 accctcaagc gtattaacca acaattcagg gattaatgga aga
//
LOCUS       DAZFZC010000273         2056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFZC010000273 DAZFZC010000000
VERSION     DAZFZC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.279"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1218
                     /locus_tag="ACHKXP_005287"
     CDS             <1..1218
                     /locus_tag="ACHKXP_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7650058.1"
                     /translation="IPLQQSTIRLNLQYPAKSGFKGEPDTCLFMLNAQGKVSGDSDFI
                     FYNNLSSPEGAVRLVTGSQQASIEIALDRVPANVSKIAITVVIDGEDTISGLSLLSIQ
                     APGIADFQAETQGRSEKAIILGEVYRHNGAWKLRALGQGFNGGLEPLAINYGVDVAQP
                     APQPAKPARISLEKKLETRSPRLVSLAKKASVSLTKNKLDTLEAAVAFVLDASGSMSG
                     QFSKGNVQSVLDRIAVLAAQFDDDGEMDVWGFGEKHKKYPNVTLDNLDTYIQSIRGAG
                     KRSSWENLPGLGGTNNEPPVMEEIVDYFKDSKIPVYVVFITDGGISKTRAIKDAIRRS
                     ANYPIFWKFVGLGGSSYGILKNLDDFTDRRVDNTHFFAMDDFGSISDEKLYDNLLEEF
                     RPWIDETKRLGIL"
     gene            1376..1801
                     /locus_tag="ACHKXP_005288"
     CDS             1376..1801
                     /locus_tag="ACHKXP_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7650059.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRCLPITTSSDTGVELLPVEITPDEPKEPMSTLPLLLSTPSQ
                     STASASSCKVEFHYDKTTLKNPSPELLTVLLRELSGRGQ"
     gene            1798..1872
                     /locus_tag="ACHKXP_005289"
                     /pseudo
     CDS             1798..1872
                     /locus_tag="ACHKXP_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          554 a          471 c          503 g          528 t
ORIGIN      
        1 ataccccttc agcaatctac gatcaggctg aatcttcagt accctgccaa atccggcttt
       61 aaaggcgaac ccgatacctg cctgttcatg cttaatgctc agggaaaggt cagtggcgat
      121 tctgacttta tcttttataa taatttgtct tctcctgagg gggcagtcag gctcgttacc
      181 ggttcgcagc aggccagcat tgagatagca ctggatcgtg ttccggcgaa cgtcagtaaa
      241 attgcgatca ccgttgtcat tgatggtgaa gataccatca gtgggctcag tttattgagc
      301 attcaggctc ctggtatcgc tgatttccag gctgagactc agggccgcag tgagaaagca
      361 atcattctgg gtgaagtata tcggcacaat ggcgcctgga agcttcgtgc gctagggcag
      421 ggtttcaacg gtggacttga gcctttggcc attaactatg gcgttgatgt cgcacagcca
      481 gctccgcagc cagcaaagcc tgctcgtatc agtctggaaa agaaactgga aacccgatct
      541 ccgcgccttg taagcctcgc taaaaaagcc tcggtcagtc tcactaaaaa taaactggac
      601 acccttgagg ctgcggttgc gttcgtactt gacgcatccg gctcgatgag tggccagttc
      661 agtaagggta acgttcagtc tgtgctggac cgcatcgccg ttcttgccgc ccagttcgac
      721 gacgacggtg aaatggatgt ctggggattt ggagagaagc ataagaagta tccaaacgtc
      781 actctggaca atctggacac gtatattcag tcgattcgag gggctggtaa gcgttcatcc
      841 tgggagaacc tgccgggcct tggggggaca aacaacgagc ctcctgtgat ggaagaaata
      901 gtcgactact ttaaggactc gaaaatcccg gtatatgttg tatttattac cgatggcggg
      961 atcagcaaga cccgggcgat taaagatgca atccggcgtt ctgccaacta ccccatcttc
     1021 tggaagtttg tcggcctggg tggttcaagc tacggtatcc tcaaaaatct ggatgacttt
     1081 actgaccgcc gggttgataa cacccacttc tttgccatgg atgatttcgg ttcgattagc
     1141 gatgaaaagt tatatgataa tttactggaa gaattcagac cgtggatcga tgaaacaaaa
     1201 aggttaggca tcctttaata catatagtta gagcatgggt gaatgagctc tacagcagct
     1261 taatactgta gagcctttct tcattaaaag gtaaacgtaa accgaccgcc atatgtagcc
     1321 attagacaag aattggtaac ttagacgccc atctgacaca gacggacatc taagtatgga
     1381 attacaggac tggcgaaaag aacctcgtaa aaagtattcg aatgaattca aacttcgtat
     1441 ggttgaactg gcatcacaac ccggtgcttg tgttgcacag attgcacgtg aaaatggcgt
     1501 caatgataat gttattttca aatggctcag gctctggcag aacgaagggc gtgtttcgcg
     1561 gtgtcttcca ataacgacct cttctgacac tggcgttgaa ttattacctg tagagataac
     1621 gcctgatgag ccgaaagaac cgatgtcgac tcttcccctg cttttatcca ctccttcaca
     1681 gagcacagcc agtgccagtt cctgtaaagt ggaattccac tacgataaaa cgacgctgaa
     1741 aaatccttca ccagagctgc tcacagtgtt gctccgtgaa ctgagcggga ggggacaatg
     1801 atctcactcc cgtcaggtac ccgcatctgg ctcgttgccg acgttaccga tatgcgtaaa
     1861 tccttcaacg gatacgacat atgctgaagg aggatcagta atggattaag tgataacaaa
     1921 tacccagaaa tagaggttca aatcctgctt agttagtaca taaatactat tttgatgaaa
     1981 attattatat gtcacctcgg attttaatca ggcgcccaga ttaaatcaag tattgagcgc
     2041 ctgattatgt ttattc
//
LOCUS       DAZFZC010000274         2032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFZC010000274 DAZFZC010000000
VERSION     DAZFZC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.280"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..183)
                     /locus_tag="ACHKXP_005290"
     CDS             complement(<1..183)
                     /locus_tag="ACHKXP_005290"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HFO7650060.1"
                     /translation="MDTIRITKCFTFDMAHALKGYDGLCRNIHGHTYMLRVTLAGKIK
                     HEDSNPKNGFVLDFGDV"
     gene            complement(207..653)
                     /locus_tag="ACHKXP_005291"
     CDS             complement(207..653)
                     /locus_tag="ACHKXP_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFO7650061.1"
                     /translation="MFTKACEYGIRACIFVALNSMEKRRVSLKEIAQSIDSPEYFTAK
                     ILQKLAKGKIIYSVQGAQGGFEMEEAKIRTISIEDIVLVIDGEVAFTMCVLGLKKCSE
                     VNPCPVHHKYKNIKADLKEMIKTTTVFDMVDQVNDGHSFLKIFELE"
     gene            complement(1045..1557)
                     /gene="dfrA36"
                     /locus_tag="ACHKXP_005292"
     CDS             complement(1045..1557)
                     /gene="dfrA36"
                     /locus_tag="ACHKXP_005292"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA36"
                     /protein_id="HFO7650062.1"
                     /translation="MKVSLIVAMDLEKGIGKNNDLMWHLPADMLFFKETTLNHIVVMG
                     RKNFESIPERFRPLPNRENAILTRNTAFEAPNCTVFHSMEGCLKHYENEDKRTVFIIG
                     GGQIYEEALEKNRVDEMFITFVDHTFGADTFFPSIDFSLWNEEVLRVHEADSKNAYNF
                     TVKKFTKKLS"
     gene            complement(1641..1922)
                     /locus_tag="ACHKXP_005293"
     CDS             complement(1641..1922)
                     /locus_tag="ACHKXP_005293"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650063.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTCFSLNSYLFPI"
BASE COUNT          630 a          409 c          382 g          611 t
ORIGIN      
        1 tacgtctcca aaatccaata cgaaaccatt tttagggttg ctgtcctcgt gtttaatttt
       61 gccagctaaa gtgactctta acatataagt atgtccatga atgtttcggc aaagaccgtc
      121 gtatcctttc aatgcgtgcg ccatgtcgaa ggtgaagcac ttggtaattc tgattgtatc
      181 cacgaatttg attgttagct gttactttat tctaattcaa aaattttcag aaaagaatgt
      241 ccgtcgttta cttgatctac catgtcgaaa accgtggtgg ttttgatcat ttctttgaga
      301 tcagctttga tgtttttgta tttatgatga acagggcaag ggtttacttc tgaacatttt
      361 ttcaaaccaa gaacgcacat cgtaaaggct acctcaccat caatgaccaa aacaatatct
      421 tcaatggaaa tcgttctaat cttcgcctcc tccatctcaa aacctccctg tgctccttga
      481 acggaataga ttatttttcc cttggcgagt ttctgaagaa tctttgcggt gaaatattcg
      541 ggtgaatcaa tagactgtgc aatttctttt aaactgacac gccttttttc catcgaatta
      601 agcgctacaa aaatacaggc acgaatgcca tactcacatg cttttgtaaa cattgttttc
      661 gtaattttcg gtaaaagtag tgaagtaatt tcaaagtgga tagattagtc taatttaaaa
      721 ttgaaattga ttgtaaaaac agtgtatttt aagatcttgg atttgagaaa aaagatgatt
      781 tatcagtttg atcgaaaaga caacaggcat tccttctatt gaagttgatt tggttcagaa
      841 cagcatgtat atcagaaaaa cagggcttct ttattattta aacaagttac agaattaaag
      901 gctgtatatc aaagtgaacg ctacgataaa agaagcactt cgacaaaata aaggttcgtg
      961 aaatttagat ttttccacct tagaagaata gcaaaaatgt tttagtgcca gcacctcaaa
     1021 ataacgttaa cccgaaaaag cgaatcagga taacttctta gtgaattttt tgaccgtaaa
     1081 attatacgca tttttagaat ctgcttcatg cacacgcagc acctcttcat tccaaagcga
     1141 aaaatcgatg gaaggaaaaa atgtatccgc accaaaagta tgatccacaa aggttataaa
     1201 catttcatca accctgtttt tttctaaagc ctcctcatat atttgtccgc caccaatgat
     1261 aaaaacggtt ctcttatctt cgttctcata gtgtttcaag caaccttcca tgctgtgaaa
     1321 aacagtacaa ttcggtgctt caaaagctgt attccgagtt aatattgcat tttcccgatt
     1381 tggaagtgga cgaaaccttt cagggattga ttcgaaattt ttcctaccca taaccacaat
     1441 gtgattcagt gtagtttctt taaaaaaaag catatcggcc ggtaaatgcc acatcaaatc
     1501 gttgttttta ccaatgccct tttcaagatc cattgcaact atcaaactta ctttcatgaa
     1561 aaacgctatt agaaaaatga atgtattata aaagtatata aattgaagca atatatcact
     1621 ttttgaaagc aatagatgga ttaaattgga aataaatagc tgtttaaaga gaagcacgtt
     1681 gtcggcagcg ggccaaaaag gaatacgtcc atgcccatcg aggtgaaacc ggctgtgagc
     1741 gcgggttcaa gcatatagcc cgacaggcgc gtatccttgc cgatcacgac acgatggcgg
     1801 tggtcaccgc gacgaaagac acggccagcc gccatgccga cgcgcaaggc ggtttccgcc
     1861 gtcatcgcgc cttcgttggc tttgccacga ataccgtctg tgccgaaata tttgcgcacc
     1921 ataaggtcga ttatcctgtc gtcgggtcgc cctcaaaggg gacatgcctg ctgaaccgcg
     1981 aatatagaga aatatcccga atgtgcagtt aacgaattct tgcggtttct tt
//
LOCUS       DAZFZC010000275         2018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFZC010000275 DAZFZC010000000
VERSION     DAZFZC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.281"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..326
                     /locus_tag="ACHKXP_005294"
     CDS             <1..326
                     /locus_tag="ACHKXP_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7650064.1"
                     /translation="ADLQPGTIEYERHRLTRAQADAQELKNARDSAEVVETAFCTFVL
                     SRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSEY
                     IEQSG"
     gene            301..>2018
                     /locus_tag="ACHKXP_005295"
     CDS             301..>2018
                     /locus_tag="ACHKXP_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7650065.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYVREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGGEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLQRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDG"
BASE COUNT          482 a          510 c          592 g          434 t
ORIGIN      
        1 aggcagatct ccagccaggg actattgagt acgaacgcca tcgacttacg cgtgcgcagg
       61 ccgacgcaca ggaactgaag aatgccagag actccgctga agtggtggaa accgcattct
      121 gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggatccccc
      181 tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg
      241 atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga
      301 gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc
      361 tcactgttcc ggccggagcc acagaccgcc gttgaatggg cggatgccaa ttactatctc
      421 ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc
      481 atgaatgcga tgggcagtga ctacgtccgc gaggtgaatg tggtgaagtc tgcccgtgtt
      541 ggttattcca aaatgctgct gggtgtttat gcctacttca tagagcataa gcagcgcaac
      601 acccttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag
      661 ccgaccatcc gcgatattcc gtcgctgctg gcgctggctc cgtggtatgg caaaaagcac
      721 cgggataaca cgctcaccat gaagcgtttt tccaatggtc gtggcttctg gtgcctgggc
      781 ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt
      841 gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttcctggg cgataagcgt
      901 attgaaggct ctgtctggcc aaagtccatc cgtggctcca cgcccaaagt gagaggcacc
      961 tgccagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc
     1021 ccgcactgcg ggggggagca gtaccttaaa tttggcgata aagagacgcc gtttggcctc
     1081 aaatggacgc cggatgaccc ctccagcgtg ttttatctct gcgagcataa cgcctgcgtc
     1141 atccgtcagc aggagctgga cttcactgat gcccgttata tctgcgaaaa gaccgggatc
     1201 tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga accgcctgac
     1261 agcgtgacct ttcacatctg gacggcgtac agcccgttca ccacctgggt gcagattgtc
     1321 aaagactgga tgaagacgaa aggggatacg ggaaaacgta aaaccttcgt gaacaccacg
     1381 ctcggtgaga cgtgggaagc gaaaatcggt gaacgtccgg atgctgaagt gatggcagag
     1441 cggaaagagc attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc
     1501 gactcccagc tggatcgcta cgaaatgcgc gtttggggat gggggccggg tgaggaaagc
     1561 tggctgattg accggcagat tattatgggc cgccacgacg atgaacagac gctgcagcgt
     1621 gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcggtatcc
     1681 cgtatctgct gggatactgg cgggattgac cctaccattg tgtatgaacg ctcgaaaaaa
     1741 catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggtaa gccggtggcc
     1801 agcatgccac gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc
     1861 gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc
     1921 ggtgccgttc acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg
     1981 actgctgaag agcaggtcga aaaatgggtg gatggcag
//
LOCUS       DAZFZC010000276         2002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFZC010000276 DAZFZC010000000
VERSION     DAZFZC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.282"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(276..575)
                     /locus_tag="ACHKXP_005296"
     CDS             complement(276..575)
                     /locus_tag="ACHKXP_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7650066.1"
                     /translation="MNTQYALKTLNQLRPVLIGFRKANGLTQKDVSERLGITQQTYAR
                     LEANPASAGFERLFRVFSVLGVEIVLSSREPLSDAKPEYGAMHNYSSLPARREKW"
     gene            complement(928..1854)
                     /locus_tag="ACHKXP_005297"
     CDS             complement(928..1854)
                     /locus_tag="ACHKXP_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650067.1"
                     /translation="MVYENELWHSFLLRSQIMYNLSNCRNIISKHGALRYDAFPRFEL
                     IDMYKLHSLQDIYDILATRNGYILTSNIVSLSSGVYGYFNAVEDACRKNFHITNSYPL
                     VNISNIRYCHKCIVEDIHSKGIGYLRHRWLFESKCAVHSTSLYEVCFDNYLNAVKGLS
                     DLIISGINPHGYCSLVVDVSNPFKNPVYILPCARNKILKWISDNKNMLIYFLFDLFKC
                     KSHSSLSKVIEVKIMHDRYISAVFKMLSEVNFCNFNSFISDIFEDVSYASIGLDDYSV
                     NVHFLRLRAADCKFCQLNGKYFCSLYNVKLLR"
BASE COUNT          669 a          396 c          357 g          580 t
ORIGIN      
        1 aaccattctt ttatgcgtaa tatttgctac tgacactgat gaatttaaaa gaagatagat
       61 tttcatttac ggattatttc ctaaattcat cgataatgtg atcgacataa aattttcact
      121 gaagaatgaa gagataattt aaggccgagc gaaatagctc aaaatggtgt atttaacata
      181 acagaagaaa tgaaaggctc tatcgcaacc tgatacttca ggccgctaag gggttaatct
      241 tgttgaagaa aaattagacc gggaagcgcc aggcgtcacc atttctcccg cctggctgga
      301 agtgaagaat aattgtgcat agctccgtat tcgggctttg catctgacaa tggttccctg
      361 gatgaaagta cgatctccac tccaaggacg ctgaacaccc taaacaaacg ctcaaaaccc
      421 gcacttgcag ggttggcctc tagtcgggca taagtttgct gtgttatacc caaacgttca
      481 gaaacatctt tttgcgtcag tccattggct ttgcgaaagc cgattaaaac aggacgtaac
      541 tgattcaaag tcttcaaagc gtattgagtg ttcataagtg accttccgta tcgacgagtt
      601 aacaagcata acgcagggat ttacagcata taggctgtaa tgctcattaa cagactatag
      661 gctgtaatac gaatcctgcc aagatgcggc atctttcaaa tacaaggccg tattgggtaa
      721 gcagtacacc gttatatagc accccttgcc cctttgctgg agagggaccc acgtgatcgc
      781 aggtctttta gacgttgttg ctaaccggca tcatacacag gtggtaaccc ccagggaaac
      841 tctatgagtg ataatttatt cctgccccag tcaaaatata aaaccttatc taactagctt
      901 tcttagtatt ttataaatga agtgaaatta gcgaagaagt tttacattgt aaagtgagca
      961 aaaatattta ccattcaact ggcaaaattt acaatctgca gcccttagcc tgagaaaatg
     1021 cacattaaca gaataatcat caagtccgat agaggcataa cttacgtcct caaaaatatc
     1081 agaaataaat gagttgaaat tacaaaaatt aacttcactt aacatcttga acaccgcaga
     1141 aatatatcta tcatgcatta tcttgacttc gataacctta gataaacttg aatgcgactt
     1201 acatttgaag agatcaaaga gaaagtaaat cagcatattt ttattgtccg aaatccattt
     1261 taaaatttta ttacgtgcac atggtaatat atacactgga tttttgaaag ggtttgaaac
     1321 gtcaaccaca agggaacaat aaccatgagg atttattcca cttataatta agtcactaag
     1381 tcctttcact gcattcaaat agttatcaaa acaaacctca tataaactgg tactatgaac
     1441 tgcacattta gattcaaaca accatctatg acgcagataa ccaatgcctt tagagtgtat
     1501 atcttcaaca atacatttgt gacagtaacg aatgtttgag atgtttacca atggatatga
     1561 attagttatg tgaaaatttt tccgacaggc atcttcaaca gcgttaaaat acccgtaaac
     1621 accagaagat aacgacacta tattggaagt tagtatataa ccatttctag tggcaagaat
     1681 gtcatatata tcctgcaagc tatgcaattt gtacatatca atcagttcga aacgaggaaa
     1741 ggcatcgtac cttagtgcgc catgctttga tattatgttc ctgcaattgg ataagttata
     1801 cattatctgt gaacgcagaa gaaaactgtg ccacaattca ttttcgtaaa ccatataact
     1861 ccctcttatt taattagttc catttttctg agaagtatca ttgtcattgg tagtttatct
     1921 ctgtatttca aaagccagcc atgccctccc agtaaagcat ctagacttgt aagtgacatc
     1981 gagtactgcc cctgagataa aa
//
LOCUS       DAZFZC010000277         1938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFZC010000277 DAZFZC010000000
VERSION     DAZFZC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.283"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1213)
                     /locus_tag="ACHKXP_005298"
     CDS             complement(<1..1213)
                     /locus_tag="ACHKXP_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HFO7650068.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
     gene            complement(1217..1777)
                     /locus_tag="ACHKXP_005299"
     CDS             complement(1217..1777)
                     /locus_tag="ACHKXP_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7650069.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
BASE COUNT          300 a          620 c          636 g          382 t
ORIGIN      
        1 cggcgcgcag ttccagcacc tccagcaagg ccggcgtgta acggcgcagg gtggcgaagt
       61 tctcgccgac caggtgcagg tggtcgaagc cttccggccg ggccagcagc tcggcctcgc
      121 tgacgctctc ggtgaactcg tcccagggaa tcaccgcctc gatggcggca taggggtcgc
      181 tgccgctttc cttcgcttcc agcagcgcct ggccgatcct ggagtacagg cgcaccttgt
      241 cgttgatcgc cttgccctgc ttctggaact gctgctgatg cttgtgcttc gcgccgctga
      301 acagcttgac caggatgcgg tcatgcagat cgaccagctc atcgatcacg gtcgcggtgc
      361 tctccagcac cacggcggcc agggtcgcgt agcggcgctg cggctcgaac ttaccgaggt
      421 ctttgggcgt catctgccca ccctcgcggg ccagcttgag caggcggttc tggtggatgt
      481 gccggcccag gccttcgggc aagtccacca actgaaatgt cttcagccgc tcgatgtgtt
      541 ccagcatgtg ccgagagttg ggtttcagcg gtgcctggcg cagccaggtc aaccaggtga
      601 tgctgctgcc ggccttgagc ttcaacagct cgtccagctt ggcccgatgc gagtccgtga
      661 gtggttcgac cagggcgcgg tagacccgcc gattggctcg cgcaatggct tccgagcagg
      721 cccggtcaat cacgctcagc gccggcagga tgcgtcgttt ctgccgtagg ctctccaggg
      781 cctgaccggc cagcagcaag cctttgtcgg tctgctgggc cagctcggtt agctcgcgca
      841 ccagggcgcg gaagtcggac aggccgaacg gggccagttg caggtaggtg cgcagttcct
      901 gggcatgctc gcgacgggtc acgtcgcgct cgccgtactt tgcccagctc gccggctcgg
      961 cctggacttg cttcgccacc cacaggatga ccggctcggg cagctcgctg tcggttccca
     1021 gcgcgtaacc ggggtagcgc agcaggcaga gctgcacggc gaagccgagg cggttggcgt
     1081 cgccgcgtcg ctggcggatc agcgacaggt cggagtcgtt gaaggtgtag tagcggatca
     1141 ggtcatcctg gctttccggc agcgcaagca gggtgtcccg ctccgtggcc gagaggatca
     1201 agcgacgcgg catgtgtcag tcgtccgtgc ggaggtactg gtagagggtt tcccggctga
     1261 tgttgaactc gcgggcaagc tgcgccttgg gctcgccggc cgtcgctcgc tgccgcaggg
     1321 tagcagcctg ctcatcggac agggctttct tgcggccccg gtacgcgcca cgctgcttgg
     1381 ccaaggcgat gccctcacgc tgccgctcgc ggatcagggc gcgctcgaac tcagcgaagg
     1441 cccccatcac cgacagcatc aggttggcca tcggcgagtc ctcgccagtg aacaccaggc
     1501 cctccttcag gaactcgatg cgcacgccgc gctgagtcag cttctgtacc aagcgacgca
     1561 ggtcatcgag gttgcgggcc agccgatcca tgctgtgcac caccactgta tcgccttcgc
     1621 ggacgaagct cagcagcgct tcgagctggg ggcgctgggt gtccttgccc gatgccttgt
     1681 cggtgaacac cttgctcacc tgggtttgtt ccagctggcg ttccgggttc tggtcaaagc
     1741 tgctgacccg gacgtagccg atgcggtgcc cctgcacgat gtctccttgg ttgaaggcgg
     1801 cttaagtgca ctttctgttc cgttgtgcct caaagcccat ttctgtcagg ctgaaatcta
     1861 taaccttcgc gggcatgtgt caaaaaatgg gaaagcagac tctattctga cgaagcggcg
     1921 cggccctgcc tgacatca
//
LOCUS       DAZFZC010000278         1871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFZC010000278 DAZFZC010000000
VERSION     DAZFZC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.284"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..458
                     /locus_tag="ACHKXP_005300"
     CDS             <1..458
                     /locus_tag="ACHKXP_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7650070.1"
                     /translation="ILSKGNIGLAQMIYRGAQLKVIGSGNEIITGAVLSASVPVLVSH
                     TEEYKDTPLPGAATEDEEAKWLSASNEGDASAKIMAEKSLKSLIPGDIDRPQHKEFVG
                     KIDEVLRNFDEKILSLDHDLVINRTAEKKNTYDALQRCGLINIDPLNKL"
     gene            499..>1871
                     /locus_tag="ACHKXP_005301"
     CDS             499..>1871
                     /locus_tag="ACHKXP_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HFO7650071.1"
                     /translation="MRMLPVLPDESLFSRFCRTTTVYGMSPSSLLTIIFNKPDMNVHP
                     ILNSGLKAISLHTSERADQLWHEQTLLPLFAWALPISRNEIMDFNTTPARLNRLCRLS
                     NFSLGQRTLLKFCPVCAREDTFHYGVTYWHLAHQLHGVTTCHRHPVALESIHVPSSPH
                     IRIGLMPPVSYTEQLSNEIDFDFAKFCYESLNIIRRKDITHPNYMDVLKKLNLLSLDG
                     NLKKNVFYAHVYAKCQLFGEGSSGLIPTSLTDYHYWEPILKDKCCQHPTKHLLLCYCL
                     LNTCWPTYAGSRTNKKKEIFKSHKKYSFHIVENNTSVSNLGKEFSRSRCYIKTLIYKK
                     YLRAFKRNTKINIFTELLIKSMAVRGFSLASIAEKNSLSEGAVSSVISSCYGLCSWRK
                     KCKKDSLRRRHKQKILRFIHNQSVSITRKLVKESCYASFYWLNKHECDWLNSCLPKTI
                     RCYKNKRV"
BASE COUNT          578 a          358 c          382 g          553 t
ORIGIN      
        1 tcattttatc taaaggcaat attggattgg ctcagatgat ttatcggggg gcacaattaa
       61 aggttatcgg tagcggcaat gaaatcatca ctggcgcagt cttgtcggcc tctgtaccgg
      121 tactcgtgag tcatacggag gaatataagg atactccact cccgggggcc gcgactgaag
      181 atgaggaagc taaatggctc tctgcatcta atgagggtga tgcttcggct aaaatcatgg
      241 cagagaagtc cctaaagtct ttgatccctg gggatataga caggccacag cacaaggaat
      301 tcgtaggaaa aatagacgag gttttgcgca atttcgacga gaaaatactg tctcttgacc
      361 acgatcttgt tatcaacaga acagctgaaa agaaaaatac ctacgatgct cttcagcgtt
      421 gcgggcttat aaatatagat ccacttaata aattgtaatg aggtaacagt tcgtaacggt
      481 caaagagagg tgtgggaaat gagaatgttg cctgttttgc ctgatgaatc cttgttcagc
      541 cggttttgtc ggacaactac cgtgtacggt atgtccccat cttctctgtt aacgatcatt
      601 ttcaacaaac ctgatatgaa cgtccatcca attctcaatt caggattaaa ggctatttct
      661 cttcatacat ccgaaagggc agatcagctc tggcatgaac agactttact ccctcttttt
      721 gcctgggcac taccaatcag tcgtaatgaa attatggatt tcaacacgac ccctgccagg
      781 cttaatcgat tgtgccgcct tagtaatttt tcactagggc agcgcacact attgaagttt
      841 tgcccggttt gcgctcgtga agatactttt cattatggtg ttacttattg gcatctggcg
      901 catcagcttc atggggttac aacctgccat cggcatccgg tagcgcttga aagcatccat
      961 gtcccttctt caccgcacat acgtattgga ctgatgcctc ctgtttcgta tacagaacaa
     1021 cttagcaatg agatagactt cgattttgct aagttttgtt atgagtccct aaatataatc
     1081 agaagaaaag atattacaca ccccaattac atggatgtac ttaaaaagtt gaatttatta
     1141 tcattggatg gaaatttaaa gaaaaatgta ttctacgcac atgtttatgc taagtgccag
     1201 ttatttgggg agggttcatc gggacttata ccaacatccc taactgatta tcattactgg
     1261 gagcctatac tcaaagacaa atgttgtcag catcccacaa agcatctttt gctttgttat
     1321 tgtttgttaa atacttgctg gccaacgtat gcaggaagtc gtactaataa aaagaaagaa
     1381 atctttaaaa gtcataagaa atacagtttt catatagttg aaaataatac tagtgttagc
     1441 aaccttggga aggaatttag tcgcagcaga tgttacatta aaacacttat ttataaaaaa
     1501 tacctgaggg cgtttaagcg aaacacaaaa attaatatat tcactgaatt gcttatcaag
     1561 tctatggctg taagggggtt tagtctggca tccatagctg agaaaaactc attatcggaa
     1621 ggagctgtat cctctgtaat ttcatcttgt tacggtttat gctcatggcg taaaaaatgt
     1681 aaaaaagatt ctttaagacg gcgtcataag cagaaaatat taagatttat acataatcaa
     1741 tccgtttcta taacacgaaa gttagtcaaa gaaagttgtt atgcaagttt ctattggcta
     1801 aataaacatg aatgtgactg gcttaattcc tgtcttccta aaacaatacg atgctataaa
     1861 aataaaagag t
//
LOCUS       DAZFZC010000279         1856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFZC010000279 DAZFZC010000000
VERSION     DAZFZC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.285"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1792
                     /locus_tag="ACHKXP_005302"
                     /pseudo
     CDS             1..1792
                     /locus_tag="ACHKXP_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
     gene            1789..>1856
                     /locus_tag="ACHKXP_005303"
     CDS             1789..>1856
                     /locus_tag="ACHKXP_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFO7650072.1"
                     /translation="MTALLTLEEIKAHLRVDHDADD"
BASE COUNT          446 a          441 c          603 g          366 t
ORIGIN      
        1 cgacgtattt tttcagaaat ggcgggcgac catcaggcgt gattgaggtc cccggcagta
       61 ttacggaaga aaacgcgaaa aaactgaagg ggaactggga cagcggatat acgggcgaaa
      121 atgccgggaa gacggccata ctgagcaacg gggcaaaata cagccccacg acgttttcac
      181 cggtggatgc gcagacggtg gaacaactga aaatgacggc tgaaattgtc tgttcggtgt
      241 tccgtgtccc ggcctacaag attggcgttg gccacccgcc ttccagtgac aacgtggagg
      301 cgctggagca gcagtattat tcccagtgtc tgcagacgct gattgagtcc attgagctgt
      361 tactggatga ggcgctggaa acgggggaaa acgagagcac ggagtttgac gtcaccacgc
      421 tgctgagaat ggacagcgaa cggcgcatga aaacactggg tgaatcggtg aaaaatacgc
      481 ttctcacgcc caatgaggcc cgtaaacgtg agaacctgcc gccccttgcc ggcggtgatg
      541 cactgtatct tcagcagcag aactacagtc tggaggcgct gtcccgtcgt gatgcccgtg
      601 aggacccgtt cgcgtcggcc gggaaaacag tttcagcaca actgcctgac ggcgcatctg
      661 acggtaataa ggcaatcagt gaaacagagc atgatgcagt gaaagcgatg ttcaggggga
      721 tactgagaaa atgacggaac gggaactgtc cattattcgt gcactgggcg aagaattctc
      781 cacggtgctg gcggatttac agcgcacatt tgaggggaag atggccgcgc aggcacaagc
      841 gtttgaagag aaactggctt ccctgtcggc ggtattacag aagcatgtga cggtggatga
      901 ggtacatccg gttctgcagg cgatggtgga tgacgctgtg gggaccattc cggtaccgcg
      961 tgatggtcgt gattatgatc cggatgtact gcagcaggcg gtgaatgatg cggttgcgaa
     1021 tattccggta ccggcggacg gcaaaagtat cacccccgat gatgtgcgtc cgatgcttga
     1081 gcagatggtg aaagaggctg taagccatat ccctgctccg cgtgacggtc gtgattatga
     1141 tccggaagta ctgaagcagg cggtgaatga tgcggttgcg aatattccgg taccggcgga
     1201 cggcaaaagt atcacccccg atgatgtgcg tccgatgctt gagcagatgg tgaaagaggc
     1261 cgtaagccat atccctgctc cgcgtgatgg tcgtgattac gatccggaag tactgaagca
     1321 ggcggttctg gaggcggtga atgcccttcc ggctccgcag gacgggcgtg atgccacggc
     1381 actggaagtg ctccccgcta ttgacgatca aaaatccttt ccccggggca cgtatgccac
     1441 acacctgggt ggactctggc gggcgtatga aaaaacgcac gggatgcggg gatgggaatg
     1501 cctggttgac ggggtggcgg atatcgacgt cagcatgacg gatgagcggt tgttctctgt
     1561 ggtgatccgg cagagcagtg gccagtgtac ggaaaaaaca ttttccctgc cggtgatgct
     1621 ctaccgcggt gtgttcagag ccggtgaaac ctaccacccc ggcgatacgg tgacgtgggg
     1681 gggctcgctg tggcactgca acagtatgac cggtgataaa cccggagaag ctcattcatc
     1741 aggctggacc ctggctgcaa aacgtggacg ggatgcagga ggcggaaaat gacggcatta
     1801 ctgacactgg aagagatcaa ggcacatctg cgtgttgacc atgacgcgga tgatga
//
LOCUS       DAZFZC010000280         1852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFZC010000280 DAZFZC010000000
VERSION     DAZFZC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.286"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..279
                     /locus_tag="ACHKXP_005304"
     CDS             <1..279
                     /locus_tag="ACHKXP_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7650073.1"
                     /translation="LAWVEKNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINA
                     GDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            572..703
                     /locus_tag="ACHKXP_005305"
     CDS             572..703
                     /locus_tag="ACHKXP_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650074.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLG"
     gene            926..1132
                     /locus_tag="ACHKXP_005306"
     CDS             926..1132
                     /locus_tag="ACHKXP_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7650075.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            1197..1421
                     /locus_tag="ACHKXP_005307"
     CDS             1197..1421
                     /locus_tag="ACHKXP_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650076.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            1778..>1852
                     /locus_tag="ACHKXP_005308"
     CDS             1778..>1852
                     /locus_tag="ACHKXP_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650077.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCH"
BASE COUNT          460 a          395 c          491 g          506 t
ORIGIN      
        1 ctggcatggg tggagaaaaa catcaaagtg ccgctgaccg aaccccagaa agcggggatc
       61 gcgtcattct gtccgtataa cattggcccc ggtaagtgtt tcccgtcgac gttttataaa
      121 cgaattaatg caggtgatcg aaaaggtgcc tgcgaagcga ttcgctggtg gattaaggac
      181 ggtggcagag actgccgtat ccgctcaaat aattgctacg gtcaggtatc ccggcgagat
      241 caggaaagtg cgctggcgtg ctggggtatc gacagataag cagaatattt tgcttaaaaa
      301 tgacgttggc caacgcgggc agataacacg aaatcctgcg aactggcaaa acccaagtga
      361 ataaaagtaa aaaccccgtt tgttggttgc aaacggggtt ttatgtttat ggcagtaagc
      421 tatgggaggc tgccttgatt gattttagca aactgattag ggagttgcga ctcatgatta
      481 gtcaattacc aaactggaaa tttttgctgg tctggagcat ccctttttta tgggtagtat
      541 cccagtgaat tgtggcaatt aaggggtagc tatgtcagac aaactcataa tgctggcgaa
      601 gatcctctgt gtaatcgtcg gcatttcatt ttcactaatg ctggttgctc tttttctttc
      661 catgggctgg atgatgttgt cttcgtcggg gctgctgggg tgagcataaa ccgaatgctt
      721 tccgcgttta ccgttattct gctggtggtc tgtggtgcgc ttagtctggg gctgaatcat
      781 taccgcgata acgccatcac ctacaaagcg cagcgcgata aaaaagccag tgagctggat
      841 ctggcaaacg caaccattac tgatatgcag gtgcgccagc gcgatgttgc tgcgctcgat
      901 gcaaaatact cgagggaatt agccgatgcg agagctgaaa atgaaactct gcgtgctgat
      961 gttgccgctg gtcgtaagcg cctgcggatc aacgccacct gccccggtac cgtgcgtgaa
     1021 gccaccggca cctccggcgt gggcaatgat gccgccgtcg aactctctcc ggttgctgga
     1081 cgaaacgttc tcggtatcag agacggaatc atcagcgacc aggcagcatt gagaatgctt
     1141 caggaatata tccgcactca gtgtattaac tagtattttt gttattcgga gaatgcatga
     1201 agaaattatt ggtaaccgta aagccttttc agggaacaat tccgttccgt attttgcagc
     1261 gtggtcgtgt tcttgttgaa ggttcgttca gtggtaaatg tacgcaatta cactccagga
     1321 cctttcaggt gaatgccacg aatgaagagc taaccgttga gtgtacgatg aatgccgcta
     1381 aatgccgcat ggtatccgct gcattacagc cagtgtgttg agcgacctta ttattcatgc
     1441 gcggtattgt cgccgtattc ctgcattaac agagaccgca gcccgaccgg gagaatcctc
     1501 tgcgcgagtg tgcggggata atcaaaaacg atacacaccg gggtttaccg cgttaacgga
     1561 gcgcggcgtt gtcccctcat ggtcgctggt ccggtgcgat ggtggaagaa gccggatgtt
     1621 tatcactatt aattgataac acagaaatgg attcattgat tttcagcacg tttttgtatt
     1681 cgtattattg aacatctgtt tattttactt ttaacatatt gataataaaa agagctgtaa
     1741 atctttagat gagtcgattt tgtccgggga agttcaaatg gattttatgc tgacggtttc
     1801 tggtgtggtt atcctgtcca ttgcttatac tgcagataaa tatggctgcc at
//
LOCUS       DAZFZC010000281         1835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFZC010000281 DAZFZC010000000
VERSION     DAZFZC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.287"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1835
                     /locus_tag="ACHKXP_005309"
                     /pseudo
     CDS             1..1835
                     /locus_tag="ACHKXP_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          646 a          359 c          335 g          495 t
ORIGIN      
        1 tattaattct tgtggagttt accctcaagg attaaaagac agagaaatta tatcaacttt
       61 tcacgcagaa aaagttatta atgatctgtt aaaaaacgat tataaaattt ccctttctcc
      121 agatacaact tatcgagagt tgaataaagc agcacagcgt agcattacag cgccagacag
      181 gataggagaa ggaaaaacat gggtttatca acgagataca atgattgaaa gaggtgataa
      241 cagcggtgtt tatcagtatg gtcctgctga acacttcacc cacattatat ctgacaaacc
      301 ttccccaaaa gataaatatg ttgcatatgc tattaacatt cctgactatg agctggcagc
      361 cgatgtatat aatattaacg tgacgtcacc ttccggacag caagaaacat ttaaaatatt
      421 aatcaatcca gaacatctac ggcaaacact tgagcgtaaa tctcttactg ctgttcagaa
      481 atcacaatgt gaaatcatca cccccaaaaa acctggcgaa gcgattattc atgcttttaa
      541 tgccacctac cagcagatca gagaaaatat gtctgaattt gcacgttgcc attatgggta
      601 tatacaaatc cctccagtga caactttccg tgccgacgga ccagaaactc ccgaagaaga
      661 aaaaggttac tggtttcacg cttatcaacc cgaagatctt tgtaccatcc acaatccaat
      721 gggagatttg caggatttta tcgcattggt taaagatgct aaaaaatttg gtatcgatat
      781 cattcctgat tataccttta actttatggg aatcgggggt agtggtaaaa atgacctgga
      841 ttatccctct gctgatatac gagcgaagat cagtaaagat atagaaagtg gtatccctgg
      901 ctattggcaa ggtcaggttt tgattccatt tactatagat ccagtaacaa aagaacgtaa
      961 acaaatccat ccagaagata tacatctcac tgcaaaagac ttcgaagcaa gtaaagataa
     1021 catctctaag gatgaatggg aaaacctcca tgcattaaaa gaaaagcgtt taaatggaat
     1081 gcctaaaaca acacccaaaa gtgaccaggt tattatgttg caaaatcaat acgttcgtga
     1141 aatgcgaaaa tatggcgtac gaggtttacg ttatgatgcg gcaaaacact caaaacatga
     1201 acaaatagaa agatcaataa ccccaccgct taaaaattat aatgagcggt tacacaatac
     1261 taacttattt aacccaaaat atcataaaaa agccgttatg aattacatgg aatatctggt
     1321 aacttgtcag ttggatgaac aacaaatgtc atcactgctt tatgaaagag atgatttaag
     1381 cgccattgat ttttcattgc tcatgaaaac gataaaagcc ttttcatttg gtggagatct
     1441 ccaaaccctt gcatcaaaac cgggttccac aatctcaagt atcccatcag aaagacggat
     1501 attgattaac attaaccacg attttcctaa caatggtaat cttttcaatg actttctatt
     1561 taaccatcaa caagatgaac aattagcaat ggcatatata gccgctctgc cgttcagcag
     1621 gcctttagtt tactgggatg gccaagtatt aaaatcaacg actgaaatta aaaattatga
     1681 tgggtccacg cgtgtcggcg gtgaggcgta gcttaataaa ggttgctcta cctatcagca
     1741 gctctacaat gaattccacg cattatatat agataaagca ggaatatgga gcgcatttga
     1801 gggtgtattt gcaactaaaa acgttctggc cttta
//
LOCUS       DAZFZC010000282         1828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFZC010000282 DAZFZC010000000
VERSION     DAZFZC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.288"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..572
                     /gene="escR"
                     /locus_tag="ACHKXP_005310"
     CDS             <1..572
                     /gene="escR"
                     /locus_tag="ACHKXP_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO7650078.1"
                     /translation="VIGTSFLKISIVLGILKNALGIQQVPPNMALTSVSLILTMFIMS
                     PIILQINDNISQEPINYTDSDFFQKVDEKILSPYRGFLKKNTEKENVEFFERAAQKKL
                     GNETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSP
                     VTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            572..841
                     /gene="escS"
                     /locus_tag="ACHKXP_005311"
     CDS             572..841
                     /gene="escS"
                     /locus_tag="ACHKXP_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO7650079.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            841..1617
                     /gene="escT"
                     /locus_tag="ACHKXP_005312"
     CDS             841..1617
                     /gene="escT"
                     /locus_tag="ACHKXP_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO7650080.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQAD
                     IAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1610..>1828
                     /locus_tag="ACHKXP_005313"
     CDS             1610..>1828
                     /locus_tag="ACHKXP_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO7650081.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVGLVNTTIDSLNRPFLYAIRE"
BASE COUNT          541 a          295 c          330 g          662 t
ORIGIN      
        1 ttgttattgg tacttcattc ctgaaaattt cgattgtatt ggggattctg aagaatgcat
       61 taggtattca acaggtacca ccaaatatgg cgctaacatc agtatctttg atactgacaa
      121 tgtttattat gtctccgata atattacaga taaatgataa tatttctcag gaaccaatca
      181 attacaccga ctctgatttt tttcaaaaag ttgatgaaaa aatattatca ccataccgcg
      241 gttttttaaa aaaaaataca gagaaagaaa atgttgagtt ttttgaacgt gcagctcaaa
      301 aaaaattggg taatgaaact atcttaaaaa aagactctct atttatactg ttaccagctt
      361 tcacgatggg gcagcttgaa gctgcattca agataggttt tttgctctat ttacctttta
      421 ttgcgataga tttgatcatt tccaatatct tattggcctt gggtatgatg atggtatcgc
      481 cagtaacaat ttcgattcct tttaagatct tgttattcat tcttgttgga ggctggcaaa
      541 aattattcga atttctgttg gtggtgaatt gatggaaact ggatattttg ttcaattatg
      601 tgtgcaaacg ctctggataa tatttatcct ctcactgcct acagtcatag cggcctctgt
      661 tgtcggcatt attattagcc tggttcaggc tataacgcag ttacaggatc aaacattgcc
      721 ttttttgcta aaaataatag cagtgtttgc tacgcttgcc ctgacttatc actggatggg
      781 aacaacaatc atcaacttct cgtcgataat atttgaaatg attccgaagg tgaatggcta
      841 atgaatgaaa taatgacggt catagtatca tcattttttt gtatactcag gccgctgggg
      901 atgtttatta ttctaccaat attttcaaca ggtgttttgc tcagtaactt tatacgcaac
      961 tcaattatga ttgcgtttac acttccaatt atagtagaga actatacatt ttcggaaaaa
     1021 ttaccatccg gaatttttca gttgacaggc atagtgctaa aagaaataag tataggcttt
     1081 ttcattgggt tatcatttac tattcttttt tgggcaatag atgcggctgg gcagattatt
     1141 gatactttaa gaggttcaac aatatcttca atttttaacc cgtctataag tgattcatct
     1201 tctatcactg gtgttatttt ataccaattt atcactgtga tctttgttat tcatggtggg
     1261 atacaaagcg ttctggataa gttgtattta tcctacgaga tattaccatt acaagccgat
     1321 attgcattca atcgtgcttt agtagatttt ttattttcgc tatgggattc atttattaaa
     1381 ctgatgttat cattttcaat tcccatgatt atcggtatct tcttatgtga tatggggttt
     1441 gggtttctca ataaaacagc gcctcagcta aacgtattca cattatcact gccggtaaaa
     1501 agtttgatag caatctttat attgttatta gttattcatg ttttccctga ttttattacg
     1561 gcaaatattc attctgacat aattgacaga tcgttaccga gcatgattaa tgagtgaaaa
     1621 aacagaaaag cccacaccta aaaaactaag ggatctaaaa aagaagggcg atgtaaccaa
     1681 aagtgaagag gtaatggctg cagtgcagtc attaatctta ttttcatttt tttctttata
     1741 tggtgcgagt ttttttgttg aggttgttgg tttagttaat acgactatag actcgctaaa
     1801 tagaccgttt ttgtatgcca ttcgtgaa
//
LOCUS       DAZFZC010000283         1823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFZC010000283 DAZFZC010000000
VERSION     DAZFZC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.289"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1012)
                     /locus_tag="ACHKXP_005314"
     CDS             complement(<1..1012)
                     /locus_tag="ACHKXP_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7650082.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLSVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPA"
     gene            complement(1420..1587)
                     /locus_tag="ACHKXP_005315"
     CDS             complement(1420..1587)
                     /locus_tag="ACHKXP_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7650083.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(1584..>1823)
                     /locus_tag="ACHKXP_005316"
     CDS             complement(1584..>1823)
                     /locus_tag="ACHKXP_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO7650084.1"
                     /translation="SEINAISATITGQLDTNFKIGRRAALREIEDVKHDTREAEDVLD
                     VAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
BASE COUNT          441 a          542 c          415 g          425 t
ORIGIN      
        1 atgccggaat atccggatct tctgccggcg cgttagtggc ggtattgaca ccgttaccgt
       61 ctgtcatgaa gggcacaaaa taaacgccct cactctccct gtttttatac ccgccgtaca
      121 cggtgtcgta ctgggtagcg tatgtatttt tccagtaata cgtcgtgtca ccacaaatcc
      181 acggcacatc tgcagcactg ccaccatggc actgcgcgtt aaacacggag aggtcagcac
      241 gaaactgtgt cagcatggct gtaaacagcg caggttgctg tgcgtgggtg gcggcgctca
      301 tgtcaaactc tccctgcatc cagcacaccg ccagcaacac atttttcggg ttcttctgta
      361 atgcagcttt agtgcgcgca atcaggtcct gatataacgg tttacccaca ccccagcgtg
      421 ccgaatcctg gctggccccc gtgtccgcac tgaatgtccc ctcagcgccc tgggtgaatg
      481 cagaaccacc acgacagcat ggtaccagca ggatccccgc gttattcggg atatacggaa
      541 gcagtttttt ggcaatatgt aacccctggc cgacacagcc gtactgccct ttgctcaggt
      601 ctgccttcgg atgattcagc gtactcatat cctgcacatc atgcaggcag tggtcggccg
      661 gaataatatc gttatatctg caggcagccc cacccggcgt aactgtactg cggcgcgcca
      721 gctgtttaat gcgcggatcc ggagcatcgt atgaatccgg aagcggaagc ccttcaccgt
      781 aagccattgc attggactgc ccggccagta cgatgacgta gtaccaatcc ggctcagttg
      841 caccactgac caccacatca ccttctgctg caatcgcctg catcagggta taaggggtta
      901 tggccaccgg actaccaaac ggctgccagc cctctttcag tttgtgtgtc agcttttccg
      961 caaggtctga cggcgacgcc gccctgacaa catcataatg tttaaatgtc attattcctc
     1021 ccggccggga tagtgtatta aatcagatat ggagtgggct gtagtccgga agcctgaatg
     1081 acacacgggg actacagccc aagaaatgaa aaaaggccac gcagttgcgc agcctgataa
     1141 accctggtta aaatccacac gataacaaca caacaatatc agtatctcat gctattgccc
     1201 gaacccattc gggcattttt ttacccataa aaaagcccct ccggagaggg gcatgtttgc
     1261 atgcacattc tttttcttgc atggtgccgg gtgcctcccg gtgaattcag tatcagcacc
     1321 tgaatccgcg attaccccat attccttctt gctgattgcc ccaccgcaca gggggattca
     1381 ccatgcagaa gtgtttttaa taaacagcaa acaaaaaaat caagcattat gcaggctgtt
     1441 tctttttatc accggccaca gcaataccac aatgccgcag accagcactc catccgccag
     1501 caccgacatg atcctgctgg tgaaatccac catcaccacc agaaacagca ggagtgccac
     1561 agcggccaga cgcagtttta ccgtcacagg tggttctcca gtcgcaggcc aagaacacca
     1621 gcaatctctt ccagaacctt acgctcttcc ggctcaattt caccatctgc ttcagcaatg
     1681 gccaccgcca catccagcac atcttccgct tcacgcgtat cgtgtttcac atcttcaatt
     1741 tcacgcaacg ctgcacgacg accaatttta aaattggtgt caagctgacc tgtgatcgtg
     1801 gcactgatgg cattaatttc tga
//
LOCUS       DAZFZC010000284         1782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFZC010000284 DAZFZC010000000
VERSION     DAZFZC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.290"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..615
                     /locus_tag="ACHKXP_005317"
     CDS             <1..615
                     /locus_tag="ACHKXP_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7650085.1"
                     /translation="AKIPVPADGKSITPDDVRPMIEQMVKEAVSHIPVPRDGRDYDPD
                     VLQKAVLEAVSALPAPQDGRDAMALEILPAIDDQKSFPRGSYATHQGGLWRAYEKTYG
                     MRGWECLVDGVADIDVSMTGERSFSVVVRQSSGQRTEKTFSLPVMLYRGVFRTGETYH
                     PGDTVTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
     gene            612..938
                     /locus_tag="ACHKXP_005318"
     CDS             612..938
                     /locus_tag="ACHKXP_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7650086.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            949..1299
                     /locus_tag="ACHKXP_005319"
     CDS             949..1299
                     /locus_tag="ACHKXP_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7650087.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            1296..1742
                     /locus_tag="ACHKXP_005320"
     CDS             1296..1742
                     /locus_tag="ACHKXP_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO7650088.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANELKDE
                     VVSRAPVRRGKLRRNVVVLSWRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
BASE COUNT          378 a          418 c          616 g          370 t
ORIGIN      
        1 gcgaaaatac cggtaccggc agacggtaaa agtatcaccc ccgatgatgt gcgtccgatg
       61 attgagcaga tggtgaaaga ggcagtgagc catattcctg ttccgcgtga tggtcgtgac
      121 tacgatccgg atgttctgca gaaggcggtt ctggaggcgg tgagtgccct gccggctccg
      181 caggacgggc gtgatgccat ggcactggaa atactccccg ccattgacga tcaaaaatcc
      241 tttccccggg gctcgtatgc cacacaccag ggtggactct ggcgggcgta tgaaaaaacg
      301 tacgggatgc ggggatggga atgcctggtt gacggggtgg cggatattga cgtcagcatg
      361 acgggtgaac ggtcgttctc tgtggtggtc cggcagagca gtggccagcg tacggaaaaa
      421 acattttccc tgccggtgat gctctaccgt ggtgtgttca gaaccggcga aacttaccac
      481 cccggcgata cggtgacgtg ggggggctcg ttgtggcact gcaacagtat gaccggtgat
      541 aagcccggag aagctcattc atcaggctgg actctggctg caaaacgtgg gcgggatgca
      601 ggaggtggaa agtgacagca ttactgacac tggaagaaat caaggcacat ctgcgtgttg
      661 accatgacgc ggatgatgag atgctgatgg acaaggttcg tcaggctacc gccgtgctgc
      721 tggcctacat ccagggcagc cgggataaag tgattagtga ggacggtgag ctgatccctg
      781 gtgaggcatt aacccggatg aagggggctg ccatgcgact gaccgggatg ctgtaccgga
      841 atccggatct tgctgagcgg gaagacctcg tgcaggggga actgccgttt tctgtttccg
      901 tgctgattta cgatttgcgt tgtccgacgg tgttatgagg agggggggat ggcaatatct
      961 gcaggtcgtc tgacacagat gatcagtgtt ctgaacccgg tgttaacccg taatgctgcc
     1021 ggagaaatga cggaagaatg ggtgtcatgc gggaaaattc atgcggatat ccggggcagg
     1081 agcagccggg agcggatgca gtccggtgcg gaaatggcgc aggcggaaat ccgcatctgg
     1141 gtgcgcggtc agtctggtcg tgaaatcacg gcggcgtcac gacttcatgt gctgagtggt
     1201 ccctggcgtg accatgtcct gaatgtcgtc ggggtcccgg ttccggatgc aaccggcggg
     1261 cgtctggaaa ttctctgtcg gctgggaggg gaaaaatgat cgaaaccctg ctggattttt
     1321 cggggctgga ggacatcagc cgcgatttgc agcttctgag tggtgcggaa aacaaccggg
     1381 tgttgcgtga ggcaacccgc gcgggggcga atgaactgaa agacgaagtg gtgtcacggg
     1441 caccggtgcg caggggaaaa ctgcgccgca atgtggtggt cctttcctgg cgttcccgcg
     1501 atggcgggat ggaatccggt gtgcatatcc gtggtgttaa tccggacacc ggcaacagtg
     1561 ataacaccat gaaggcggat aatccccgca atgccttcta ctggcggttt gtggaaatgg
     1621 ggaccgtgaa tatgccaccg cacccgtttg tgcgcccggc atttgatgtg cgcagtgaac
     1681 aggcggcaca ggtggcgatt gcgcggatga accgggccat tgatgaggta ctgagacgat
     1741 gacggaggcg gatttgtatc ctcatctggc gcatcttgcc gg
//
LOCUS       DAZFZC010000285         1765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFZC010000285 DAZFZC010000000
VERSION     DAZFZC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.291"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..220)
                     /locus_tag="ACHKXP_005321"
     CDS             complement(<1..220)
                     /locus_tag="ACHKXP_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7650089.1"
                     /translation="MAIKGLEQAIENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARI"
     gene            complement(232..585)
                     /locus_tag="ACHKXP_005322"
     CDS             complement(232..585)
                     /locus_tag="ACHKXP_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7650090.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLNIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(597..992)
                     /locus_tag="ACHKXP_005323"
     CDS             complement(597..992)
                     /locus_tag="ACHKXP_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7650091.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1034..>1765)
                     /locus_tag="ACHKXP_005324"
     CDS             complement(1034..>1765)
                     /locus_tag="ACHKXP_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7650092.1"
                     /translation="QNLADPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTM
                     TGEAFDPVEVDMGRSVANNITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVF
                     DPKGWALFRSFKAVKEKLDTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYV
                     ENDVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAR
                     EFTMIQSAPLMLLADPDAFVSVQLA"
BASE COUNT          349 a          559 c          456 g          401 t
ORIGIN      
        1 tgattctggc ctgcggattt ttgaccgtgg cccttttcag cctggccctt tcctttacca
       61 gtttccggcg tacctttgtc tcacgggcaa cctgtgacgc cgactgcgat atcgcggatg
      121 aagcaacgcg gttaatggcc attgcggcgg caccaggcac cgccgttttg ctgatacggc
      181 tgaggttttc aatggcctgc tcaagacctt ttatggccat acatccccct ttcagcggcg
      241 acggttaacg gcaggcggta cgccccgccc aagccagaga tgacagcttc caccatcatc
      301 cggcgaaacc cggtctatcc agaagttttc ctcaccgatg ttcagcgtgt ctccacgccg
      361 cagctgccgc acatcatcag tccggacaaa cagggacggg ctggagcctt caacgcgcac
      421 gccctgtccg gcatagctga tattttcagg gtcatcaaaa acaccacgta ttactgcgcc
      481 ggactgctca ccggatgtca tggtggctga cgttcccatg taccctcgta tcgtttcatc
      541 ggcgcggaca atggcagcat cgaacaggtt atcgaaatca gccacagcgc ctcccgttat
      601 tgcattctgg ccaggccgcg ctctgtcatt tcggctgcca caccggcaga gacacggaac
      661 gccgttcccg gcagcacaaa tgccacagcc tcatcccgcg tggcgtgaag tgcatcagta
      721 tgcagcgtca ccagtgccac aaccgtgacc agatcagccg tatcagtcac ggtatccggc
      781 tgcgctgata caacctcatt ttcatgtccg gtcagcgcat tttccgggct gacagatgtg
      841 tcctgaccag cagcgtcatc cgtgtcatca agctcctctt ccagctctgc cacacggagt
      901 gccagttctt ctttcgtccc cgtcaggctg acatcacggt tcagttgctc acccagcacc
      961 tgaagacggg caatcagttc atctttcgtc atggactcct ccacagagag aaaatggccc
     1021 cgaagggcca tgattacgcc agttgtacgg acacgaacgc atcagggtca gccagcagca
     1081 tcagcggtgc tgactgaatc atggtgaact cacgcgccgg atcgccggtg gtcacccagt
     1141 ttttcgggta gcgggcagag gcgttaatac cttcgcgctg tgcgtccgca tcctgaatgc
     1201 agccataggt gcgcagaccg cgtgcctgag tgttccccag caccatcgtg ttgtccggca
     1261 ggaagttctt tttgacgtcg ttttccacgt actgtccgga atacacgacg atcgccgtat
     1321 cgccatacat ccccttatag gacaccgctt cgcccaggtc ttttaccgct gtctccagct
     1381 cggaattaga gccgcgacgg gtatccagct tctccttgac ggctttgaag gaacggaaca
     1441 gcgcccagcc tttcggatca aacacgatga tattcaccac accgctggcg ttcagcgcgt
     1501 aggcttcgat atcgtcggtc gggtcatacg tggacttgtc acgcttgctc cactccgtac
     1561 cgccggactg cgtgatgttg ttcgccacac tgcggcccat atccacttca accggatcga
     1621 aggcttcacc ggtcatggta tatttgccct taagcacggc agaaacggcc tgcatctctt
     1681 cgacctgagc aatggccagc tcttcgtctc gcatgttctg caggatgatg cgacggcggc
     1741 ggtaagccgg gtccgccaga ttctg
//
LOCUS       DAZFZC010000286         1765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFZC010000286 DAZFZC010000000
VERSION     DAZFZC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.292"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..220)
                     /locus_tag="ACHKXP_005325"
     CDS             complement(<1..220)
                     /locus_tag="ACHKXP_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7650093.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARI"
     gene            complement(232..585)
                     /locus_tag="ACHKXP_005326"
     CDS             complement(232..585)
                     /locus_tag="ACHKXP_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7650094.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(597..992)
                     /locus_tag="ACHKXP_005327"
     CDS             complement(597..992)
                     /locus_tag="ACHKXP_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7650095.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1034..>1765)
                     /locus_tag="ACHKXP_005328"
     CDS             complement(1034..>1765)
                     /locus_tag="ACHKXP_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7650096.1"
                     /translation="QNLADPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTM
                     TGEAFDPVEVDMGRSAANNITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVF
                     DPKGWALFRSFKAVREKLDTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYV
                     ENGVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAR
                     EFTMIQSAPLMLLADPDEFVSVQLA"
BASE COUNT          347 a          560 c          459 g          399 t
ORIGIN      
        1 tgattctggc ctgcggattt ttgaccgtgg cccttttcag cctggccctt tcctttacca
       61 gtttccggcg tacctttgtc tcacgggcaa cctgtgacgc cgactgcgat atcgcggatg
      121 aagcaacgcg gttaatggcc attgcggcgg caccgggcac cgccgttctg ctgatacggc
      181 tgaggttttc aacggcctgc tcaagacctt ttatggccat acatccccct ttcagcggcg
      241 acggttaacg gcaggcggta cgccccgccc aagccagaga tgacagcttc cgccatcatc
      301 cggcgaaatc cggtctatcc agaagttttc ctcaccgatg gtcagcgtgt cgccgcgccg
      361 cagctgccgc acatcatcag tccggacaaa cagggacggg ctggagcctt caacgcgtac
      421 gccctgtccg gcatagctga tattttcagg gtcatcaaaa acaccacgta ttactgcgcc
      481 ggactgctca ccggatgtca tggtggctga cgttcccatg tacccgcgta tcgtttcatc
      541 ggcgcaggca atggcagcat cgaacaggtt atcgaaatca gccacagcgc ctcccgttat
      601 tgcattctgg ccaggccgcg ctctgtcatt tcagctgcca caccggcaga gacacggaac
      661 gccgttcccg gcagcacaaa tgccacagcc tcatcccgcg tggcgtgaag tgcatcagta
      721 tgcagcgtca ccagtgccac aaccgtgacc agagcagccg tatcagtcac ggtatccgtc
      781 tgtgctgata ccacctcatt ttcatgtccg gtcagtgcat tttccgggct gacagacgtg
      841 tcctgaccgg ctgcgtcatc cgtgtcatca agctcctctt ccagctctgc cacacggagc
      901 gccagttctt ctttcgtccc cgtcaggctg acatcacggt tcagttgctc acccagcgac
      961 cggagacggg caatcagttc atctttcgtc atgaactcct ccacagagag aaaatggccc
     1021 cgaagggcca cgattacgcc agttgtacgg aaacgaactc atcagggtca gccagcagca
     1081 tcagcggtgc tgactgaatc atggtgaact cacgcgccgg atcgccggtg gtcacccagt
     1141 ttttcgggta acgggcagag gcgttaatgc cttcgcgctg tgcgtccgca tcctgaatgc
     1201 agccataggt gcgcagaccg cgagcctgag tgttccccag caccatcgtg ttgtccggca
     1261 ggaagttctt tttgacgccg ttttccacgt actgtccgga atacacgacg atggccacat
     1321 cgccatacat tcccttatag gacaccgctt tgcccaggtc ttttaccgct gtctccagct
     1381 cggaattaga gccgcgacgg gtatccagct tctccctgac ggctttgaag gaacggaaca
     1441 gcgcccagcc tttcggatca aacacgatga tattcaccac tccgctggcg ttcagcgcat
     1501 aggcttcgat atcgtcggtc gggtcatacg tggacttgtc acgcttgctc cactccgtac
     1561 caccggactg tgtgatgttg ttcgccgcac tgcggcccat atccacctca accggatcga
     1621 aggcttcacc ggtcatggtg tatttgccct taagcacagc agaaactgcc tgcatctctt
     1681 cgacctgagc aatggccagc tcttcgtctc gcatgttctg catgatgatg cgacggcggc
     1741 ggtaagccgg gtccgccaga ttctg
//
LOCUS       DAZFZC010000287         1739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFZC010000287 DAZFZC010000000
VERSION     DAZFZC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.293"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..1665
                     /gene="tir"
                     /locus_tag="ACHKXP_005329"
     CDS             49..1665
                     /gene="tir"
                     /locus_tag="ACHKXP_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO7650097.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPTTTDPDTAASTAEAATKDRLT
                     QEAFQDPDKQKVNIDENGNAIPSGELRDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPTSRRNSNASPVSNWSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          483 a          373 c          458 g          425 t
ORIGIN      
        1 atataaagga ttattatcta tttacataaa ataaaaagga gatattttat gcctattggt
       61 aatcttggcc acaatcccaa tgtgagagct ttaattccac ctgctccgcc attaccttca
      121 caaaccgacg gtgcaggagg tgcccgtaat cagctcatta actcaaatgg cccgatgggg
      181 tctcgtttgc tatttacgcc tataaggaat tctgttgctg atgctgctga ttctcgtgcc
      241 agtgatattc ccggacttcc tacaaatcca ctgcgctttg ctgcgtccga ggtatctttg
      301 catggtgcgc ttgaagttct tcatgataaa ggggggcttg atactcttaa ctctgctatt
      361 ggatcttcgt tattccgtgt tgaaactcgg gatgatggca gccatgttgc tatcgggcaa
      421 aaaaatggcc tcgagaccac tgttgtttta agtgagcaag agttttctag cttacagtcc
      481 cttgatcctg aaggtaaaaa caaatttgta tttactggag gccgcggtgg cgcagggcat
      541 gctatggtca cggttgcttc agatatcgcc gaagcccgtc agaggataat agataaatta
      601 gaaccaaagg atacaaagga gacgaaggag ccaggggatc caaatagtgg cgagggaaaa
      661 atcattgaaa ttcatacctc aacctcaact tctagcctcc gtgcagatcc taaactttgg
      721 ttgtcattgg ggactattgc tgcaggtctg atagggatgg ctgcgacggg tattgcacag
      781 gctgttgcgt tgactccaga gccggatgat ccaactacta ccgatcctga tactgcagca
      841 agcacagctg aagctgcgac aaaagatcgg ttaacgcagg aggcattcca ggatccagat
      901 aaacaaaaag ttaatatcga tgagaacgga aatgcaattc cgtcagggga attaagagat
      961 gatgttgttg cgcaaatagc agaacaagct aaagcggcgg gggaacaggc cagacaggaa
     1021 gctattgaaa gtaattctca ggcgcagcaa aaatatgatg agcagcatgc taaacgcgaa
     1081 caggaaatgt ctctttcatc gggggttggc tacggtatta gtggggcgct gattcttggc
     1141 gggggaattg gtgccggtgt tactgctgct cttcatcgga aaaaccaacc ggcagaacaa
     1201 acaatcacta cacgtacggt agtcgataat cagcctacga ataacgcatc tgcgcagggc
     1261 aatactgaca caagtgggcc agaagagtcc ccgacgagca gacgtaattc gaatgccagc
     1321 cccgtatcga actggtctga cacctccagc acgggcacgg tagagaatcc gtatgctgac
     1381 gttggaatgc ccagaaatga ttcactggct cgcatttcag aggaacctat ttatgatgag
     1441 gtcgctgcag atcctaatta tagcgtcatt caacattttt cagggaacag cccagttacc
     1501 ggaaggttag tgggaacccc agggcaaggt atccaaagta cttatgcgct tctggcaagc
     1561 agcggcggat tgcgtttagg tatgggagga ttaacggggg gtggcgagag cgcagtaagt
     1621 actgccaatg ctgcaccaac gccgggaccc gcacgtttcg tttaaatata tccgtgagta
     1681 tttagttgag gtcgggaggg ggggagtttt actaacatta atgtttcaga gaataaagt
//
LOCUS       DAZFZC010000288         1730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFZC010000288 DAZFZC010000000
VERSION     DAZFZC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.294"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(7..843)
                     /locus_tag="ACHKXP_005330"
     CDS             complement(7..843)
                     /locus_tag="ACHKXP_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO7650098.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(843..1646)
                     /locus_tag="ACHKXP_005331"
                     /note="aph(3'')-Ib"
     CDS             complement(843..1646)
                     /locus_tag="ACHKXP_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO7650099.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          414 a          500 c          455 g          361 t
ORIGIN      
        1 tgatatctag tatgacgtct gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg
       61 ttgctcctct tctccatccg cgttccaagc tgcggaaagg cacccataag cgtacgcctg
      121 gtcgagcagg cgacgcggat cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc
      181 aatgcgtcta ggatcgagac aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc
      241 ggcgccaaag cccacttcac cgaccagacc gacgggatct atcaccagcc agccgcgact
      301 ggagaacatg atgttttcat gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt
      361 gctcatcatt tgatcggcta taatcgccgc gtggacgtag tcagtttgac aacctgcgtt
      421 ttgatcatcg cgcgcccgct gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc
      481 agaaggcagg ggttcctcag atgcggcata cagcttcgcc attagttccg ctgcaatttc
      541 ggtcgcctgg tagtcgccgt gctcggcaac gatgtgagag agcattcgct ccccggcata
      601 ttcgagcaac atcagattgt tctcacgacc gagcaaccgg actgctcccc tcccattgcg
      661 ccataccaga tagtcggccc cgcgcagttc atcagcaatg tcttctatag gtttcaatcc
      721 cttgacgatt gcaggagtcc cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt
      781 gtccgcaatg agaacaggtt gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa
      841 catcaacccc aagtcagagg gtccaatcgc agatagaagg caaggcgttc gcggtcgggg
      901 gcttcgatcc ccaatacatt gaataggaca gcgaaggcgc gctctgcttc atctggcgct
      961 gcccagttct cttcggcgtt agcaatcatg agtgccaaat cggcatagcg atctgctgtt
     1021 ccgagccgcc caaggtcgat cagacccgtg cattgaagag ttttagggtc caccatgaag
     1081 ttcggcatgc agggatcacc atggcaaaca accatatcgg tgcgctcttg gtcgagccgc
     1141 accggtagct ctcgttcgac acgagccaaa agatcgagct gcggcgtact cttgtcctcg
     1201 tccggtaaga agtcgggatt gacggcattg cgggacacca catcaacggc gcgtccgaac
     1261 attcgcgaca gcctgcgctc aaacggacat tgatcaaccg ataggctgtg aacagcgcca
     1321 agttgctgcc ccattgacgg ccacgctttg agcaaatccg ctccagacag atcagccgcc
     1381 ggtactcccg gaattgccgt tatcaccaag catgcaccct cctgttcctc ctgccagttg
     1441 atgacctcgg ggcaagccac acctcgacct ttgagccaaa tgaggcggtc acgctctcca
     1501 gcgagctcac cgcggcggga agcaggtgcg attttcgcga aggcatgccc gtcaccacgt
     1561 cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca accagtcaga atgcgattca
     1621 ccaaaaaaaa tattagttcg attcaatgga ggttccttca gttttctgat gaagcgcgaa
     1681 tatagagaaa tatcccgaat gtgcagttaa cgaattcttg cggtttcttt
//
LOCUS       DAZFZC010000289         1714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFZC010000289 DAZFZC010000000
VERSION     DAZFZC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.296"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..272)
                     /locus_tag="ACHKXP_005332"
     CDS             complement(<1..272)
                     /locus_tag="ACHKXP_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7650100.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMA"
     gene            complement(269..1012)
                     /locus_tag="ACHKXP_005333"
     CDS             complement(269..1012)
                     /locus_tag="ACHKXP_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7650101.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHK
                     FRCVPHLTGRLFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHASAFTGICNDLAAASACM"
     gene            complement(1023..1712)
                     /locus_tag="ACHKXP_005334"
     CDS             complement(1023..1712)
                     /locus_tag="ACHKXP_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7650102.1"
                     /translation="MHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNEKGE
                     AVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVR
                     RRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDGALF
                     PGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANF
                     GGFLSINKLSQ"
BASE COUNT          368 a          590 c          420 g          336 t
ORIGIN      
        1 aacgccattg atgccccggc agtgaagaaa gagcccacga tggctgccgc ccccagcaca
       61 atctgcagtc caccctttcc ggccccggcc agtcgcggca caatatggat gaccgccccc
      121 tcacccagct gttcgtgaag acgggcgtac accgcctccg gtgccgtgtc ctcaccgcga
      181 atacgtatct ggtaccagcc ttcgttcatc tgacggcgga atcccggcac ctgtaacgac
      241 agcgcccgga tggcttccgc tgccgtgttc acatacaggc tgaggcggcg gccaaatcgt
      301 tgcaaatccc cgtgaaggca gatgcgtgcc agtggcggtg acgccaggct gaatgcgttc
      361 gtcgttgcca tttttcggaa tacctctccc gtttactcag ttgttcaggc agatggtgaa
      421 gcagctcacc gttgccgcag tatatggcgg catgattggc caccgatgcg ccaaagcagc
      481 acagcaggat atcgcccgcc tgtgcagagg acaggggcac ccggtaaaag ccggtgaccg
      541 ccatattgtc caggtaaagg ttctgaccgt tacgccacca gtcatcctca cgctcaaaat
      601 ccggcatatc aattcccgcc agatggtagg catcccggaa cagcgtgtaa cagtccgtca
      661 ccccgtgctc aaagagccgt cctgtcagat gtggcacaca gcggaattta tgaatgtccc
      721 cccggcagac cagccaccag gacagtgcac tttttatctg cagccgccgg tcggcctcgc
      781 tcagccaggg cagaccaccg ggatgactgt ggaccagtgc cacaatctcc ccctgcatct
      841 ctgcccgcag ccagtcttcc ggtgcaatac gaaaatacgc ctccggctct gcagagatat
      901 tcacacaagg gatataccgc tccccctccg gcgttctcac cacgaagcca cacgactccg
      961 caggcacaca ccgccgggca tgcgccagaa tcgctgattc agtctgtgtc ataaaccggg
     1021 atttactgcg aaagtttatt aatggaaagg aaaccgccaa aattagccac catgccgcgc
     1081 atctcacacc cgcgcatgca cttgctgcat ctgtccttac ggatatccgt agtggggttg
     1141 tcgaactcat ccgccaccgc aggaccgtta tacccgcatt catctccccg gtaatcccac
     1201 atacaggtgt tcgccagcat gatgcgacca ggaaacagcg ccccgtccgt ctccgtcggt
     1261 gttgccagca caaacgaggc tgtcatggcc gtcagctctg acatctgctc caccacccag
     1321 cggtcgctca gctcctgctc cgggtccgct tccggattgc ccgccacaaa attcaccgca
     1381 tccagaaaac gggcatacac ccggcggcgg accaccgtgg cccccaccag actctgcagg
     1441 tcctccgcca tcccggtgac cagaccaaac agattggaca ccgtcagcga cggtctggca
     1501 ctgctgccct tcccgttcat ctcaaaaccg ctgccgtcaa tcgggtatac ctgatattgc
     1561 cgcccctgcc aggtgaccgc ctcccctttt tcattcagct cattacagaa aaaataccgc
     1621 tcaccgccct gcaccgtcag gtcaatttcc cagagcacca cccgcggtga ctgctctgat
     1681 ttaaccgact cgttcaggct ttcttcgtga atat
//
LOCUS       DAZFZC010000290         1706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFZC010000290 DAZFZC010000000
VERSION     DAZFZC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.297"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..850
                     /locus_tag="ACHKXP_005335"
     CDS             <1..850
                     /locus_tag="ACHKXP_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650103.1"
                     /translation="INDKNGCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIR
                     KNKINDVLQHYDDMERKGLFGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLD
                     HVKAYQDAFIETIPLIDPVYKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPY
                     ITGLLDDVEKVLDKINILIDKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKK
                     YTLLQCVEKLGVLVHYVKSPVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRN
                     NPPELS"
BASE COUNT          513 a          242 c          364 g          587 t
ORIGIN      
        1 tattaatgat aagaatggtt gtgatgacgc atatctgaaa aagattataa gctattttgg
       61 gcagttgcag aatgaggctg ttcatgtgac cagctgctac gaaacacttg cggtgtacat
      121 ttgtctgata aggaaaaata aaattaatga tgtgctgcag cattatgatg acatggaaag
      181 aaaaggattg tttggtgaat taccttcagg atatgtcagg ggggcattat cgttgcttcg
      241 gacagcactg gaggttaaag taaatagaaa aaatataaag tatggctcgt tgttttattg
      301 gcttgaccat gtaaaagcct atcaggatgc atttattgaa acaataccac ttattgatcc
      361 tgtatataag gagggtgaaa tacagtatga tgcgaacaat tttaccctga tgcgtgttat
      421 taagatgtat aattgtatgc ttgaaaagat cagtacgaaa ccatatattg cgcccccata
      481 tattacaggt ttgcttgatg acgtagagaa ggtgctggat aagataaata ttctgataga
      541 taaagagtat gtttatgatg gtaagacgct ggctgaagtc attatggaaa acaaagtatt
      601 atccagtcgc gaacgtaaag aaactatgat agggcttttc acaggcagta agaaatatac
      661 tttactgcaa tgtgttgaga agcttggtgt attagttcac tatgtgaaat caccggttga
      721 tgaaattaaa aatgtgatga tgttgtatgg cgataaggcc gaaaacagga acagacgcag
      781 aatgatatat gatgcgttaa ccattatttg tgaagatgac atcaggaata accctcctga
      841 gctgtcgtga attcatctga gtgtgttttg ggccaataat ctttatatac cctgctaatt
      901 ttgctaacca ttagcaaaat tagcaggggt attatctatg tctggcaaaa gaaaaatatg
      961 taacactttg atttgtctgt ttattttgtt tttcaattaa aatagtatgt ttttgtgatc
     1021 cgcatctaat tatttcgtac tcctggtgct atcccattca tggatagatg tgcatactgc
     1081 ctccattaaa ctgatggatt gcatgttcat tgcatctgta aaatatgcca gttttgacat
     1141 taatacataa tttgtaattc ggtgtaagct cttgtctggt gaattctgtt agtagcttat
     1201 acttcttgtc catttttaca ggttatgcaa gtgtgattat cctgattact ggtcacttag
     1261 tatatactga gtcaacaaaa caaatgagta ttaactgaag tttttatata gcgattattt
     1321 ttcttgttgg gttagttagt tcccagagaa ctacatatgg tttcatatac catgaataat
     1381 taaggttaat tagtattatt aatgggggtg gtggtattgt gtggtggtat ctgtggtaca
     1441 acttacctct ttttatcgta atgtcactcc tctttatgct gagtattctg tatgtgcgta
     1501 atgcgtctat ccccatatgc tacagtgtgg tggtgtatgg gatttagtta tgtgtaaatt
     1561 atgttactac actcatagag taatattgag aaagttaatc cgctgaatgt atgtctggtt
     1621 tgcatttaac actggttacg taccagaaca ctattagagt atattgtctt tagttcgtac
     1681 gttatagctc cgttacactt tataag
//
LOCUS       DAZFZC010000291         1692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFZC010000291 DAZFZC010000000
VERSION     DAZFZC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.299"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..101)
                     /locus_tag="ACHKXP_005336"
     CDS             complement(<1..101)
                     /locus_tag="ACHKXP_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7650104.1"
                     /translation="MSLQRIFIAGHRGMVGSAITRQLEQRGDVELVLR"
     gene            complement(105..1223)
                     /gene="gmd"
                     /locus_tag="ACHKXP_005337"
     CDS             complement(105..1223)
                     /gene="gmd"
                     /locus_tag="ACHKXP_005337"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO7650105.1"
                     /translation="MTKVALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHSCNPKLHLHYGDLTDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGFNVSLSQE"
BASE COUNT          460 a          397 c          377 g          458 t
ORIGIN      
        1 cgtaatacca gttccacatc accgcgctgt tcgagctgac gggtgatagc ggaaccgacc
       61 atcccgcgat gaccagcaat aaaaattcgt tgtaaagaca ttagttattc ctgggataag
      121 ctaacattaa atccatgaga tttaagaaga gagtgttttt tcgccgcttc gaggtcatta
      181 gccaccattt cagacaccat ctctctgagg gtgatttccg gtttccagcc cagtttttcg
      241 tgcgctttgg tcgggtcgcc gagcagcgtt tcaacttctg ccggacggaa gtaacgcggg
      301 tcaacggcga taatcacatc gcccggttta acgcccggcg cgtcatgccc ggtgacggaa
      361 accacaatgc ccttctcttc aacacccgtg ccttcaaagc gcagtttgat gcccagctgt
      421 gccgccgcca tttccacgaa ctgacgtacg gagtactgaa cgccggtagc aataacgaaa
      481 tcttctggct gttcctgttg cagcatcatc cactgcattt ttacgtagtc tttggcatgg
      541 ccccagtcac gcagggaatc catattgccg aggtacaggc acgactccag cccctgggcg
      601 atgttggcga ttgcgcgggt aattttgcgg gtaacgaagg tttcgccacg gcgcggggat
      661 tcatggttga agagaatacc gttacaggca tacatgccgt aggattcgcg gtagttaacg
      721 gtgatccagt aggcgtacag tttggcgacc gcatacggag atcgcgggta gaacggcgtg
      781 gtttctttct gcggaatttc ctgcaccaga ccgtacagtt cagaggtgga agcctgatag
      841 aaacgggttt tcttttccag accgaggaag cggatcgcct ccagcaggcg cagcgtaccc
      901 atcgcatcga cgtctgcggt atattctggt gactcaaaag aaaccgcaac gtggctcatt
      961 gcgcccaggt tgtacacttc atccggctgc acttcacgca aaatgcgcgt caggttggag
     1021 gtatcagtca gatcaccata atgcagatgg agtttaggat tgcaagagtg cgggtcctga
     1081 taaatgtgat ccacacgctc ggtattgaac gacgatgcac gacgtttaat accatgcacc
     1141 tcgtaacctt tttccagcag aaactctgcc agatatgagc catcttgtcc ggttatgcca
     1201 gtaataagtg ctactttagt catagtaatt cctaatacat gttttttagt taaaatttgg
     1261 taatattata attgagttct ggttttaaag gagaacagat tacaaaccaa atgacgcaat
     1321 gatttaatat gcttacatat tagataacat tcgaaaatgc tttaccaata catataaaca
     1381 acttaatata tatttctaca ttacatctct gattcatgat gcataacacc taaggtaaat
     1441 tgataacaat aacaaacgct caataactcc aaatgaataa cttaatttaa cagaggtcat
     1501 gattagtatt aagacttaat gatgacccag caattcaaag caaaaaaaat taattagcgt
     1561 tgatatccat tcgggttttt tgattgccaa ttccaaacgt cttgtaacat ttcctcaaga
     1621 tcatacttgg ctttccagtt aagtatttta tttgccttat cagtatttgc ccaagatttt
     1681 gccaaatcac ca
//
LOCUS       DAZFZC010000292         1670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFZC010000292 DAZFZC010000000
VERSION     DAZFZC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.301"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            423..638
                     /locus_tag="ACHKXP_005338"
     CDS             423..638
                     /locus_tag="ACHKXP_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7650106.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            638..1135
                     /gene="rrrD"
                     /locus_tag="ACHKXP_005339"
     CDS             638..1135
                     /gene="rrrD"
                     /locus_tag="ACHKXP_005339"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7650107.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKSACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            1132..1569
                     /locus_tag="ACHKXP_005340"
     CDS             1132..1569
                     /locus_tag="ACHKXP_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7650108.1"
                     /translation="MSRVTVIISALVICIIACLSWAVNLYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          447 a          376 c          432 g          415 t
ORIGIN      
        1 ctgacctgaa aaagaagacg ctggctaaaa ggccttatat gtttgtagag acttattttt
       61 cacagacagc aatgatgcct gtcaatatat tatcaatatg cggattgttt cagttacaga
      121 tgccttatta aggaaaaaaa cagccagcac tgactttcgg tggagaggtg ctggctcaaa
      181 aggatagatg tacttcacat gttgcttcta tatggcagta cattttctga cagacagtga
      241 cggatgttgt caagatattg tgtcatttat aacctgaatc aggggaggcc ggaatgttat
      301 ctggcatttt tagcagagcc tgaatgccat aatcacggct cccggcgttg gccgtcagtg
      361 ggtgacactg gcggcttttt tgtttttctt tactttcatt ttctgtcggc ggtgacggag
      421 acatgcatca gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggga
      481 cgggatactg gttactgcag ctgctggaca aagtctctcc gtcccagtgg gtggcaatag
      541 gtgtgctggg aagtctgctg tttggcctgc tgacgtatct gacaaatctt tatttcaaga
      601 ttaaagaaga taagcgtaag gctgcgagag gtgaataatg tcgccgtcat tacgcaaggc
      661 tgttgcagct gctattggtg gtggggctgt tgccatagcg tctgtgctca tcactggtcc
      721 aggtggtaac gatggtctgg aaggtgtcag ctacatacca tacaaagata tcgttggcgt
      781 atggactgta tgtcacggac acaccggaaa agacatcatg cccggtaaaa cgtataccga
      841 agcagaatgc aaagccctcc tgaataaaga ccttgccacg gtcgccagac aaattaaccc
      901 gtacatcaac gtcgatatac cggaaacaac gcgcggcgct ctttactcgt tcgtttacaa
      961 cgtgggcgct ggcaatttca gaacatcgac gcttcttcgc aaaataaacc agggcgatat
     1021 caaaagcgca tgtgatcagc tacggcgctg gacatacgct ggcggtaagc aatggaaagg
     1081 gctgatgact cgccgcgaga ttgagcgtga agtctgtttg tgggggcaac aatgagcagg
     1141 gtaaccgtta ttatctccgc tctggttatc tgcattatcg cctgcctgtc atgggctgtt
     1201 aatctttacc gtgataacgc catcgcctac aaagagcagc gcgataaagc cacatccatc
     1261 atcgctgata tgcagaagcg gcaacgtgat gtagcagaac ttgacgccag atacacaaag
     1321 gagcttgctg atgctaatgc gactatcgaa agtctccgtg ctgatgtttc tgctgggcgt
     1381 aagcgcctgc aagtctccgc cacctgtgca aagtcaacga ccggagccag cagcatgggc
     1441 gatggagaaa gcccaggact tacagcagat gctgaactca attattaccg tctccgaggt
     1501 ggaatcgaca agataaccgc gcaggttaac tacctgcagg aatacatcag gacgcagtgc
     1561 ttaaaataat tttaatttca ctgaaattta acaagtgact ttcaggaaaa tgcctcgcag
     1621 atgcggggca tttttgtacc ggtatttcac cgcgcaccgc agcgcacaat
//
LOCUS       DAZFZC010000293         1650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFZC010000293 DAZFZC010000000
VERSION     DAZFZC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.302"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..232
                     /locus_tag="ACHKXP_005341"
     CDS             <1..232
                     /locus_tag="ACHKXP_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO7650109.1"
                     /translation="DLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEARG
                     AWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            189..635
                     /locus_tag="ACHKXP_005342"
     CDS             189..635
                     /locus_tag="ACHKXP_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7650110.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKANMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            632..1159
                     /locus_tag="ACHKXP_005343"
     CDS             632..1159
                     /locus_tag="ACHKXP_005343"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7650111.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEVDDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMIVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1156..1338
                     /locus_tag="ACHKXP_005344"
     CDS             1156..1338
                     /locus_tag="ACHKXP_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7650112.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
BASE COUNT          461 a          365 c          453 g          371 t
ORIGIN      
        1 cgacctatca ttaacccagc caaagctaaa agaatgtccg ttttgcggcg gtaatgctcg
       61 tctgtgggtt gaggccggaa taaatattga tgtgtggggc tatgcagaat gtgacctctg
      121 tgaagccagg ggggcatggg caccatcagt tgctgcggcg gctgaaaaat ggaaccggag
      181 agcaggagat gaagcaaacc tttctgcttc gcaacgaagc aatcagaaat aacgccatag
      241 acgccattct ctcactaccc atcgacgaca agtcacccca cgaagtccac gttaaagaac
      301 ccaagcgcag caaagcgcag aatgaccgca tgtggccgat gctgaacgat gtttcgcgtc
      361 aggtgctatg gcatggtcaa cggctggcgc cggaagactg gaaagacctg ttcactgccc
      421 tgtggcttaa gaccaaaaaa ctggagcaaa gaagtgcgcc tggtatcgac ggtggcgttg
      481 tcatgcttgg cgtgcgtacc agcaaaatgc gaaaggccaa catgactgag cttatcgaaa
      541 tcatgttctg gttcggctca gagcgcaacg tgcggtggag tgatgactcc cggcgagagt
      601 atgaatggtc acaacgaaaa ggtagggctg catgactatc aaatcaaata caccagcaca
      661 cgacaaggac tgctggcaaa cgccgctttg gctttttgat gcactggata ttgagtttgg
      721 attctggctg gattcagctg cgagcgacaa aaacgctctg tgcgctcact ggctaactga
      781 ggtcgacgac gcgctcaatt ctgagtgggt aagccacggt gcaatctgga ataacccacc
      841 gtacagcaat atcaggccgt gggtggaaaa agccgctgag cagtgcatac aacagcgaca
      901 gacggtagtg atgattgtgc cagaggatat gtcagtcgga tggttcagca aggctctgga
      961 gagtgtcgac gaagttcgca tcatcactga tggacggatt aattttatcg aaccatcgac
     1021 agggctggag aagaagggaa acagcaaagg ctccatgctg ctgatttggc gaccgttcat
     1081 cagtcctcga cgaatgttta ctactgtatc caaagcggca ttgatggcga tcgggcaggg
     1141 cgtcaggagg gcggcatgag gcgacagcga cgaagtttca ccgacatcat ctgcgaaaac
     1201 tgcaaatacc ttccaacgaa acgctccaga aataaacgca agccaatccc aaaagaatct
     1261 gacgtaaaaa ccttcaatta cacggctcac ctgtgggata tccggtggct aagacatcgt
     1321 gcgaggaaaa caaggtgatt gactaaaatc gaagttacga acaagaaagc gtcgagcggg
     1381 cttcagtgta cactgagtgg attctatcta ggcttagtgc atacagaaga ttgctggtaa
     1441 aggacatgcc aggcaaaacg atgaggactg atatttatga aaacatctga ttttttactg
     1501 ttcttgcatg cggtacagga agggctttga ccgggcattt tatcgtgaat attttcactt
     1561 ggtatttctt tggttttaga gattacttca ctcgatgggt tttaaatagt tttcgtcggt
     1621 ttatcgggtg caagcctgat atgagaattt
//
LOCUS       DAZFZC010000294         1636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFZC010000294 DAZFZC010000000
VERSION     DAZFZC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.303"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            97..312
                     /gene="essD"
                     /locus_tag="ACHKXP_005345"
     CDS             97..312
                     /gene="essD"
                     /locus_tag="ACHKXP_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO7650113.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            317..628
                     /locus_tag="ACHKXP_005346"
     CDS             317..628
                     /locus_tag="ACHKXP_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO7650114.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            625..1158
                     /locus_tag="ACHKXP_005347"
     CDS             625..1158
                     /locus_tag="ACHKXP_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7650115.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1155..>1636
                     /locus_tag="ACHKXP_005348"
     CDS             1155..>1636
                     /locus_tag="ACHKXP_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO7650116.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     "
BASE COUNT          461 a          343 c          432 g          400 t
ORIGIN      
        1 tagcgccgtt gagaacaacg tgtgctgtca aaactacccc gtagactccg atcttttcaa
       61 acatattgca ccatccgtgt acatcggggt gaggatatga aatcaatgga taagttaaca
      121 acaggtgttg cctatggcac atcggcgggt aatgctggtt tctgggcatt gcagttactc
      181 gataaagtaa ctccgtcaca gtgggctgca atcggtgtgc tgggtagcct ggtttttggc
      241 ctgctgacgt atctgacaaa tctttatttc aagattaaag aagacaggcg taaggctgcg
      301 agaggagagt aatccaatga ctcaagacta tgaactggtt gtgaaaggag tccgtaattt
      361 tgagaataaa gttacggtaa ctgtagcctt acaggacaaa gaacgctttg acggtgaaat
      421 ttttgacctg gatgtcgcca tggaccgtgt tgaaggagct gcgctggagt tttatgaggc
      481 agcagccaga aggagcgtcc ggcaagtctt cctggaagta gcagaaaaat tgtcagaaaa
      541 agttgagtct tatctgcagc atcagtactc ctttaagatt gaaaatcctg ccaataagca
      601 cgagcgtcct catcataaat atctatgaac acaaaaatca gatacggcct gtcggctgcc
      661 gttctggcgc tgattggtgc tggcgcatct gctcctcaga tacttgacca gtttctggac
      721 gaaaaagaag gtaaccacac aatggcatac cgcgatggtt ctggcatatg gaccatctgt
      781 cggggtgcca cagtggtgga tggaaaaacc gtttttccca atatgaaact gtcgaaggaa
      841 aaatgcgacc aggtcaacgc cattgagcgt gataaggcgc tggcatgggt ggagcgcaat
      901 attaaagtac cactgaccga accacaaaaa gcgggtatcg cgtcattttg tccctataac
      961 attggccccg gtaagtgttt cccgtcgacg ttttataagc ggctgaatgc tggtgatcgt
     1021 aaaggtgcat gcgaagcgat tcgctggtgg attaaggatg gcggacgcga ttgccgcatt
     1081 cgttcaaata actgttacgg tcaggttatt cgtcgtgacc aggagagcgc attaacctgc
     1141 tgggggatag aacagtgaat cagatattca tggtgatttt tctcgtgttg tcaggattta
     1201 tcgtcggaaa tgtctggagc gaccgaggat ggcaaaaaaa atgggcggaa cgtgatgctg
     1261 ccgcattatc acaagaggta aatgctcaat ttgctgctcg aataattgaa caggggcgaa
     1321 ctatagcccg tgatgaggct gttaaagatg cgcaacagaa atctgctgaa atttctgcca
     1381 gggctgctta tctgtctgat agtgttaacc agttgcgtgc cgaagcaaaa aaatatgcca
     1441 tacgccttga cgcagcgaag cataccgcag atcttgccgc tgccgtcaga ggcaaaacaa
     1501 ccaaaaccgc cgaaggaatg ctcaccaaca tgctcggaga tattgcagca gaagctcagc
     1561 tttatgctga aattgctgac gaacgctaca tcgcaggagt gacttgtcaa cagatctatg
     1621 aatctttaag agataa
//
LOCUS       DAZFZC010000295         1628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFZC010000295 DAZFZC010000000
VERSION     DAZFZC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.304"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..910)
                     /locus_tag="ACHKXP_005349"
     CDS             complement(<1..910)
                     /locus_tag="ACHKXP_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7650117.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQK"
     gene            887..1030
                     /locus_tag="ACHKXP_005350"
                     /pseudo
     CDS             887..1030
                     /locus_tag="ACHKXP_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1225..1392)
                     /locus_tag="ACHKXP_005351"
     CDS             complement(1225..1392)
                     /locus_tag="ACHKXP_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7650118.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(1389..>1628)
                     /locus_tag="ACHKXP_005352"
     CDS             complement(1389..>1628)
                     /locus_tag="ACHKXP_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO7650119.1"
                     /translation="SEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTREAEDVLD
                     VAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
BASE COUNT          399 a          500 c          368 g          361 t
ORIGIN      
        1 tcttctgctt ccagaaatac gtcgtatctc cacatatcca gggaacaccg ccagcagagc
       61 caccgacgca ctgacctgcc atatccgcca ggtctgcacg gaatttatca accagcgcac
      121 caaactgtgc tgcgtgattt gccggcgtac cgccaaaatc aaattccccc tgcatccaca
      181 ccacggcaaa cagcacattt ttcggattct tcttcagtgc tgcttttgtt cgaccgataa
      241 ggtccttata cagcggcttg tccacacccc agcgggttga attctccgag gcaccactcg
      301 cgtcactgta tgtgccatcg gctccggtgg tgaacgctga accaccacga cagcacggaa
      361 ccagcagaat gcccgcattc gccggtataa acggcagcaa ttttttggcg atatgcagcc
      421 cttgccccac ggttccgtac tgcccctttg acaggtccgc tttcggatgg ttaaggcggc
      481 tcatgtcctg cacatcatgc agacaatggt ccgccggaat gatgtcgtta tatttacagg
      541 cgacaccgcc cggtgtcacc gtactgcgac gcgccagctg cttaatgcgc gggtccggac
      601 ggtcatatgt ctccggcagc ggaaggcctt caccatatgc catgctgttt gactgccccg
      661 ccagaaccac aacaaagtaa tactccgggt ctctggtggc gctgattact gtgccttctc
      721 catccgacgg cttcaccaac acaggtgtgg tgacatcacc ttccgccgca atggcctgca
      781 tcagagtata aggcgtgatg gccaccgggc tgccaaatgg ctgccacccc tccttcagtt
      841 tttgtgtcag cttttccgca aggtctgacg gcgacgccgc cctgacaaca tcataatgtt
      901 taatcgacat cgaatttctc ccgtgtacag gaacagagtt aaaaagccgg aaccggaatc
      961 aaattacagg atggccatct gccagtggct ggtcgtaaaa aaaaggccgc gccatgcgca
     1021 gccgaaaata aagggataac gatgatagtt tgagaaaaac agaaataaca cttttgtggc
     1081 aaagcatggt gccgggtgcc tcccggtgaa ttcagtatca gcacctgaat ccgcgattat
     1141 cccatatacc tggttgctga tcgcccctcc gcacaggggg attcaccatg cagaagtgtt
     1201 tttaataaac agcaaacaaa aaaatcaagc attatgcagg ctgtttcttt ttatcaccgg
     1261 ccacagcaat accacaatgc cgcagaccag caccccatcc gccagcaccg acatgattct
     1321 gctggtgaaa tccaccatca ccaccagaaa cagcaggagt gcagccacag ccaggcgcag
     1381 ttttaccgtc acaggtgatt ctccagacga agacccagaa caccggcaat ctcttccagc
     1441 accttgcgct cttccggctc aatttcgccg tctgcctccg caatggccac cgccacatcc
     1501 agcacatctt ccgcttcacg cgtatcgtgt ttcacatcct caatctcgcg taacgcggca
     1561 cgacgaccaa ttttaaaatt ggtatccagc tgaccgataa tagttgcgct aatcgcatta
     1621 atttccga
//
LOCUS       DAZFZC010000296         1593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFZC010000296 DAZFZC010000000
VERSION     DAZFZC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.306"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..891)
                     /gene="repC"
                     /locus_tag="ACHKXP_005353"
     CDS             complement(<1..891)
                     /gene="repC"
                     /locus_tag="ACHKXP_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="HFO7650120.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDI
                     TRPKAAGCPPHSIVNKTFLSF"
     gene            complement(878..>1593)
                     /locus_tag="ACHKXP_005354"
     CDS             complement(878..>1593)
                     /locus_tag="ACHKXP_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO7650121.1"
                     /translation="KSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHR
                     LHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRR
                     FHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVL
                     VDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVL
                     KPAVLERQRKSKGVPRGEA"
BASE COUNT          248 a          548 c          507 g          290 t
ORIGIN      
        1 aaaagataaa aatgtcttgt ttacaataga gtgggggggg cagcctgccg ccttgggccg
       61 ggtgatgtcg tacttgcccg ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc
      121 cggcaacgcc tcgcgcaccc gctggcggcg cttgcgcatg gtcgaaccac tggcctctga
      181 cggccagaca tagccgcaca aggtatctat ggaagccttg ccggttttgc cggggtcgat
      241 ccagccacac agccgctggt gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc
      301 gtccatgctg atgcgcacat gctggccgcc acccatgacg gcctgcgcga tcaaggggtt
      361 cagggccacg tacaggcgcc cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa
      421 cccctgccgc ttgcggccat tctgggcgat gatggatacc ttccaaaggc gctcgatgca
      481 gtcctgtatg tgcttgagcg ccccaccact atcgacctct gccccgattt cctttgccag
      541 cgcccgatag ctacctttga ccaccatggc atcagcggtg acggcctccc acttgggttc
      601 caggaacagc cggagctgcc gtccgccttc ggtcttgggt tccgggccaa gcactaggcc
      661 attaggccca gccatggcca ccagcccttg caggatgcgc agatcatcag cgcccagcgg
      721 ctccgggccg ctgaactcga tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt
      781 gctgcgcttg cgctcgcccc gcttgagggc acggaacagg ccgggggcca gacagtgcgc
      841 cgggtcgtgc cggacgtggc tgaggctgtg cttgttctta ggcttcacca cggggcaccc
      901 ccttgctctt gcgctgcctc tccagcacgg cgggcttgag caccccgccg tcatgccgcc
      961 tgaaccaccg atcagcgaac ggtgcgccat agttggcctt gctcacaccg aagcggacga
     1021 agaaccggcg ctggtcgtcg tccacacccc attcctcggc ctcggcgctg gtcatgctcg
     1081 acaggtagga ctgccagcgg atgttatcga ccagtaccga gctgccccgg ctggcctgct
     1141 gctggtcgcc tgcgcccatc atggccgcgc ccttgctggc atggtgcagg aacacgatag
     1201 agcacccggt atcggcggcg atggcctcca tgcgaccgat gacctgggcc atggggccgc
     1261 tggcgttttc ttcctcgatg tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc
     1321 cctcggcggc gcgcttgagg ccgtcgaacc actccggggc catgatgttg ggcaggctgc
     1381 cgatcagcgg ctggatcagc aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt
     1441 gcgccccaag ggcgtgcagg cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt
     1501 agatcaccgg gccggtgggc agttcgccca cctccagcag atccggcccg cctgcaatct
     1561 gtgcggccag ttgcagggcc agcatggatt tac
//
LOCUS       DAZFZC010000297         1584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFZC010000297 DAZFZC010000000
VERSION     DAZFZC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.307"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..>1584
                     /locus_tag="ACHKXP_005355"
     CDS             15..>1584
                     /locus_tag="ACHKXP_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650122.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDGTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENESAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYPKLIDEVKYCISNKKITGSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRT
                     IGRGVSYADIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLS
                     LMSSLSGAIMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNP
                     DILNTQAIALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQ
                     GERAILNTSQSETCK"
BASE COUNT          582 a          318 c          250 g          434 t
ORIGIN      
        1 attcaggagg aaacttgatt ccaaattcaa cttcaaataa aggttatgta tgcattgaca
       61 tgcagtgttc gtcaacctct gaaccaacag cttctacctc cagcaaccgg agtattaaat
      121 tagccgcttc cacaaatgta tatccgataa caagaaacga ctccgagctc actctgaacg
      181 attttcttga taatagctct tctacgtcat cattggacta cattaatgaa ttgggttctc
      241 aactgacgtt aaatgatttt cttgacaaca taaagacaaa tgaggtggat ggaacatgta
      301 cggatgtggt aattaatatc ccacaagaga tacaaacaaa tacacaggaa aatgatttgt
      361 tattatccga taaaaataat tcaatatgca ttgaaatcga tgaaagaatt acaaaaatcc
      421 taacatgcaa gcaaaaatat caactggaca gcatcattca tgaaattata ccaaaagaga
      481 atgagagtgc agaaactgtt ctccatctta tgagagttct gaacgatcaa tatcatcagg
      541 tatataatca gtcaggatgt ttttataaag cctatatggc catacacaat aaaatcgaac
      601 agatacttcc atatgcgttc agagccggag gcggaatcag cattcacttg ctcatacagg
      661 cattattttt taatggcgac tataacaaat caccttcaca gtctcaacaa ccatctttat
      721 atacatcacc ttctccaaca ataaatacag aagcattctt aagtaatgta ttatcactag
      781 atataaccca ggtacgcata cttggtgatt tactatcagc aactttattt catgcaccaa
      841 caatattcta tcaatatcct aaactaatag atgaagttaa gtattgtata agtaataaaa
      901 aaataacagg ttcggttata gcacgattta ctctatgttt aacaagtaca ttactaacca
      961 tgtcaccact gttaatgctt aatggagcag ttaaaacagg tagcatagta agaactatag
     1021 gtaggggagt gagttatgct gatataccat tggccttagc tatattaggt gactcatggt
     1081 ataaagctta taaacatggt tcttctgata acccaaattc tgctcagaga tttatatcgc
     1141 aagaagcggc ctttaaaacc acccagcggg tattaacaca aggattaagt ctgatgtcct
     1201 ctttatcggg agcaatcatg cgctctcttg agaaaggcac accaccacaa atgatgtctt
     1261 tattcatcgt aaacatacta aatctattat ttcatcaaaa tccatatgaa ggggcatcag
     1321 caagtgctaa tgctttgaaa agatcaactt actcccataa tccggacata ctaaatactc
     1381 aggcaatagc tctttgtgtt gacctccagc atacaaaaaa tataaccatg ccacttttca
     1441 aaacaaaaga caggatatcc tacgcattca atggacaaag aacatcccca gaagaccaaa
     1501 aacaaatact gaaagaagtt ataaactcct gtacccaagg agaaagagcc attttaaata
     1561 catcacaatc agaaacatgc aaac
//
LOCUS       DAZFZC010000298         1583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFZC010000298 DAZFZC010000000
VERSION     DAZFZC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.308"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..250)
                     /gene="ybfB"
                     /locus_tag="ACHKXP_005356"
     CDS             complement(<1..250)
                     /gene="ybfB"
                     /locus_tag="ACHKXP_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO7650123.1"
                     /translation="MLFFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMSYG
                     MMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAII"
     gene            complement(257..>1583)
                     /gene="rhsC"
                     /locus_tag="ACHKXP_005357"
     CDS             complement(257..>1583)
                     /gene="rhsC"
                     /locus_tag="ACHKXP_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFO7650124.1"
                     /translation="IRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRR
                     VAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIR
                     VETATGELARTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESR
                     RWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWG
                     NLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFY
                     QYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYLCVPDGKGDYT
                     CGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKGKFKEVRPRYS
                     VLPDIFTPINLGLFKNCQDWSNDSLETCKMKCFGNNIGRFIRFVFTGVM"
BASE COUNT          378 a          440 c          345 g          420 t
ORIGIN      
        1 ttattatcgc cataatatac ttactatcaa tccttacgcc aataatgtct gatacagtcc
       61 ctataaatat tagagcaata aagaatacta tgcctgttgg aaaacatatt accatcattc
      121 cataagacat gagactaatt aactcgctca catcacgagt ggagtctaat agtgaaagtc
      181 tatgcatgga aaagaatagt attaaaagcg ataaagccag ccatatagct ctgaataaaa
      241 aaaataatat atttcattac atcactccgg tgaatacaaa tctaataaaa cgtccaatgt
      301 tatttccgaa gcacttcatc ttacatgttt ctaaagaatc attagaccag tcttggcagt
      361 ttttaaatag ccctaaattt ataggtgtga atatatcagg caatacagaa taacgcggcc
      421 ttacctcttt aaacttccct tttaaacagt tctccacaca actattatcg gtttcaacga
      481 ggtcacaacg gccagcagct tcttttgtat cattacttgc tttacctggc ccccatagac
      541 catctccttt tgattctccc cgttggtctt ggccaccaca agtatagtcc cctttaccat
      601 ctgggacaca aagatattga tgatagaacg gattccccca tatatctgga ccgcttcttt
      661 caccagttcc gcccattgaa tgcagtggct taatgcattt tagtgtttct aatcctaatg
      721 gatctatatt tgagatcgga ttcaacggat actgataaaa attccatccc cccttcagcc
      781 caatcggatc ctgagtgata taccgcccct gcagcgggtc ataatagcgg tggcggttgt
      841 aatacaggcc ggactcctca tcatactgct gccccggcag ccggataagc tgctgcagct
      901 gatgcgggtt ctcttcattc agcaggttgc cccattcatc atattctgcg caccacgctg
      961 ttgccccttc cgtgctgatg agcgccagcg gcaggccgcg atggtcgcag tggtacaggt
     1021 ggatttttcg cgccggcgta tacaccgggt ccatctggtt ttgcatctgc tccacggtca
     1081 ggccgcacga tgccagccag cggcggcttt cctcactcac ccggtcagcc aggatttcac
     1141 tttccagccg gtcgagcatc tgcaccagca ccggcgggaa caccacactg ccaccgtctt
     1201 cgccgccgga ctgctgaagc gcatccgcca ggctgcggcg ctgcgttctg gccagttcac
     1261 cggtggcagt ttcaactctg atgagtggtg tgaagctccc cggctgataa atcgtctgga
     1321 tgcggctcct gtcgttctgt atcgtggtca gccggtcgcc gtcccagccg taccaggtca
     1381 cttgcggttt ccgtgacagc gacatccagc ccgtcaggtc ccgttcacgt cgccataccc
     1441 gttttgccac cctgcggccc agcgggtcgt aaagatagcg actttcgacc agcggctctg
     1501 catattgtgt ccgcgtgtag tgcaccagcc ggtgctgact gtcgtaatgg taccggtggg
     1561 tgcgctcatc atccgtacgg ata
//
LOCUS       DAZFZC010000299         1580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFZC010000299 DAZFZC010000000
VERSION     DAZFZC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.309"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(181..453)
                     /locus_tag="ACHKXP_005358"
                     /pseudo
     CDS             complement(181..453)
                     /locus_tag="ACHKXP_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(625..1002)
                     /locus_tag="ACHKXP_005359"
     CDS             complement(625..1002)
                     /locus_tag="ACHKXP_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650125.1"
                     /translation="MKYICFKELPYCDSDSDSDSDSDISIIESKGMMDKKHIDDVLRY
                     LRGGRIFSISPGLAFDIFSNAKIPIGTLEIYTDGEWCWYSDLIYYIEKYHISLPLEFM
                     NKMESKKWICDCNNIDISNTELD"
     gene            complement(1413..1580)
                     /locus_tag="ACHKXP_005360"
                     /pseudo
     CDS             complement(1413..1580)
                     /locus_tag="ACHKXP_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          432 a          394 c          300 g          454 t
ORIGIN      
        1 cacatttttc accccgtgta aaaaatattc attaggaagg aaatatgtat agtcagtatc
       61 atctttaaaa tacatacgat taccccgcgt taaaaaaaat cggtgcatgc agcccagatt
      121 acattacctt gaccagcatt taccatttga gataaataag ttgaagtgtt caccgttact
      181 gaattcaatg gcccgtctgc cctgagtggt gacattcacc cggccgcgtt cgtcgtagcc
      241 ggtgcgctgt gatgtgccgt ccgggctgaa gatacccgtc agcctgccgg atttgtcata
      301 cgcatactgc gttttccctt ccggggtgat gacttctgcc aggcggcctg cggcgtcccg
      361 gctgtagagg atggtttcct ccaggccgtt gaccctggcg gtacacaggc catcggcatc
      421 ataacggtaa cgccagacca cgccgcccca gtcacccggg tagtcggtgt ctgcgggcag
      481 actgagtgcc agcccctaca gacgcagact ccccggtcac cagaccatct ttccttttcc
      541 gttagatagc gatataagga atagctatct agtcatctcg atagtccaca tatatcttga
      601 ttcgtacact ttgattgcaa gtaattaatc taactcagta tttgatatgt caatattatt
      661 gcaatcacaa atccattttt tactctccat tttattcata aattcaagag gtaaggagat
      721 atgatatttt tcaatgtaat agattaaatc agaataccag caccattctc catcagtata
      781 tatttccaat gtgccaatag gaatttttgc atttgaaaat atatcaaaag caagtccggg
      841 tgatattgaa aatatacgtc cacctcttaa atatctcaat acatcatcga tgtgtttttt
      901 atccatcatc cccttagatt ctattatact gatatcacta tcactatcac tatcactatc
      961 actatcacaa tagggtagtt ctttaaaaca aatatatttc atttagtaac cctcggctcg
     1021 aacacgctcc aggagccagt attagatgtc gatgttttac ctacagtaat ttttcctgaa
     1081 ggaaagatcc ataatgtatc actcggtgct gtaactggca caccaagttt gttggataaa
     1141 tgttgagcaa tagcagttgg cgatgctcct gaactacatg agagaagtcg tatgggttca
     1201 cctgaatggc ctgatttttt tattagatta gcaagtgttc gatgatttat ctgaacatat
     1261 ttaccattgt gtaatacatg gaaagcatcc ggcgaaccat ggattagaac atcaaatacg
     1321 ccatcttgct tttttatcca aggtaacgcc ttaagtaaag gatctccctt gcccaccagt
     1381 gcgacatcta caccagctgc aaccgcacct ttacttaatc catatgagtc aatccacccc
     1441 agtggattag gcacataccg gtaagtctgc tcacccccct gcaaccccag tggatcactc
     1501 accaggtaca tcccgctctc cggttcgtaa taccggaacc ggttatagca cagcccggat
     1561 tctccatccc gccactgtcc
//
LOCUS       DAZFZC010000300         1576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFZC010000300 DAZFZC010000000
VERSION     DAZFZC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.310"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(62..487)
                     /locus_tag="ACHKXP_005361"
     CDS             complement(62..487)
                     /locus_tag="ACHKXP_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7650126.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(471..713)
                     /locus_tag="ACHKXP_005362"
     CDS             complement(471..713)
                     /locus_tag="ACHKXP_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7650127.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            1105..1443
                     /locus_tag="ACHKXP_005363"
     CDS             1105..1443
                     /locus_tag="ACHKXP_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7650128.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPNRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
BASE COUNT          435 a          392 c          324 g          425 t
ORIGIN      
        1 tctgataccg aatcttttta ctcacgagtt gccggaagcg attcgatatg gctattgtca
       61 gtcaatgtac tgccacagca tttcctgccg ggccaccacg gttcatctga ttgaaaccgg
      121 cgattgccac tgcgacaaaa tcatcagcgt ctctcaccag tcgctcccgc gtctccacca
      181 gctcccgaaa ataagctgaa ctgtggctgc gcattctggc caccagcaaa ggtggcattg
      241 ccttttcgat cgctggtaac aacgcctgaa ttttttcaac tgcatcaggg gtatctttct
      301 ctatccagcg gaaaattttc tgggtattgc gagccagggc ttccggatgg ctgtcgtcgt
      361 acagttcagg aaacgtcata cccaactcaa aataagcctg ggttattcca gctgccggaa
      421 ctttttcgcc atcaggacgc gcccaggcat tcatcgccat gcggatgtgt tcatgcttga
      481 ttttcatgaa tccccccttg gttagaaggc ggattatgat cagaaccggg aatgacaacc
      541 gtcggtatgt gtaactcata tttgagcgcc ccggcagtga ctgcctgaat tagcaacgcc
      601 catttccacg gaacctcttc cccccacatg ctgactgtgg tttttgacgt tcctagagct
      661 gcggctgttt taacaactcc gccaaaatag cctaatactt ctgatttttt catgagtcgc
      721 tccataaaac tgaacgccaa aagtttaata atcaaaacaa aagaaagtca agaaacaaaa
      781 ccatctgtgt tttaaaatca aaacatgagc aagcaaacaa tatctgaacg cataacccaa
      841 cgtatgcatg cgctaaacct gaaaggcaaa gaccttgtca atgccactgg cgcatcaaaa
      901 ggctccgtaa gtcaatggat gaacggtgga ggagcgccgt cctcgcgtta cataagttca
      961 ctggcaaaaa tattgaaagt aaacgaaaat tggcttctta atggaggaga gttaaataca
     1021 ggtgattcgc ttgatctatc tttaccgccg ataaaaacgg ttccgctact atcacttcag
     1081 caggcagcaa gctggagtga ttatatgaaa aattcctcaa taacctcttg tgtgcagctt
     1141 gtcggagaaa tcccggccaa tacctttgca gttgttctag agagtgacag tatgtcaaca
     1201 tctggtgggg gagtttccat cccaaatggt tcaacagttt ttgttgatcc caatcgaacc
     1261 gtacaaccag gaaatattgt ccttgcctta cccaaaggga ccacaacacc tgtcattcgt
     1321 aaactggaga tagaagggcc ggatattctt ttagtcccca cgaatcctcg ctacccttca
     1381 attatgctgg atgatctatc ttgcatattg ggcgtatgct ttaaaattca acaagatatt
     1441 taaccgacct catctatttg attaactgta tgccatcgtg gtgatggctt aacagctgcc
     1501 tgcttaaaat gttttgataa aaaaacattg acctgaaaag ttcatttttc taaacttcat
     1561 tcattccctc tccccc
//
LOCUS       DAZFZC010000301         1566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFZC010000301 DAZFZC010000000
VERSION     DAZFZC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.311"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..586)
                     /locus_tag="ACHKXP_005364"
                     /pseudo
     CDS             complement(1..586)
                     /locus_tag="ACHKXP_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
     gene            complement(750..860)
                     /locus_tag="ACHKXP_005365"
                     /pseudo
     CDS             complement(750..860)
                     /locus_tag="ACHKXP_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(907..1194)
                     /gene="nleC"
                     /locus_tag="ACHKXP_005366"
                     /pseudo
     CDS             complement(907..1194)
                     /gene="nleC"
                     /locus_tag="ACHKXP_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
     gene            complement(1228..>1566)
                     /gene="nleB2"
                     /locus_tag="ACHKXP_005367"
     CDS             complement(1228..>1566)
                     /gene="nleB2"
                     /locus_tag="ACHKXP_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO7650129.1"
                     /translation="IYLDMDMILTGKLGTIYAPDGISMHVDRRNDSVNIENSAIIVNR
                     SNHPALLEGLSFMHSKVDAHPYYDGLGKGVKKYFNFTPLHNYNHFCDFIEFNHPNIIM
                     NTSQYTCSSW"
BASE COUNT          487 a          352 c          265 g          462 t
ORIGIN      
        1 actcccggca ccataatact ggttgaaaca acgaacttcg cttgtcacct cttcatggct
       61 ttgagatata gtaaacatct tcaacacttt tgttgtattt tccatatctt catacacgac
      121 agcattacca cctttaccaa taacgttacc tagcacgggt gatttgttgt tgctaagcct
      181 cagcaacgct gactgagtat aatccgctgg cggtaactcc ggctgcgcat aatccactgg
      241 aggtaaatca ggtctgttcg aacgaacaga tcctctttct atagtcgtgc tcactgtcga
      301 tgaattcagc atagcctcaa tttttctggt gatctctccc ttaggccatc ccagtctata
      361 cagcatatta gtaaaacatc cactatgact ctctcttgtc acgcaaaacc tattatcact
      421 taaaacaacg cgatatgttc tgttgctaac ctttacttgc gccccattat cagagtgagc
      481 ggcagcatcc cttacagagg gataaaacac gattatcagg caaagtcagg tttctggtta
      541 aagaattcca tgaacacccc aaatttacag aagatggtga tagcatacat ttcaaccttc
      601 aaaatgaatc aatctttact ttcttaacaa acatcaccat gacatgacaa caaaaaactg
      661 gaaattaact attgtttttg agtatctggc aactcattca tccttccagc atcccgtcag
      721 acgaacgttt acgaactata aatcaggtat tactggccct cgaaatctgg agatactgac
      781 gtatcaacac ggttcagtaa cacccgatac ctcttccaga ctgtcaacaa cgatgtctct
      841 tcatccgttg cgatctgcag catcctgaat atgaatgcca tagcttacgg catcacgaaa
      901 agtttatgag cggctcaaag catcaagtat agtattacta atcatatcga ccgtatgctg
      961 atcaatgagt ctgccacgac tgtcatggac ggcgcgtatt acactttcgt atacgttgct
     1021 taaatctgct gcggaaagtg gtattcgctt acctatatcc aaaacgtaat ccgcataggc
     1081 attttcagca cgattaggag caatgggagc agagtatcct gccggggcgg aaaagttgaa
     1141 gttggactgt aatgagggaa ttttcatgtt tatatctaat accctgcaaa tcatttatta
     1201 caacaaaata aactatatca aattcattta ccatgaactg catgtatact gacttgtgtt
     1261 catgattata ttagggtggt taaactcaat aaagtcacaa aaatgattat aattatgtaa
     1321 tggtgtaaaa ttaaaatatt tcttaactcc tttccccaaa ccatcataat atggatgagc
     1381 atctacttta ctatgcataa aagaaagtcc ctcaagtaga gcaggatgat tactacggtt
     1441 aacaattatt gcactatttt caatatttac actatcatta cgacgatcca catgcattga
     1501 aattccatca ggagcatata ttgtaccaag cttacctgta agtatcatat ccatatcaag
     1561 atagat
//
LOCUS       DAZFZC010000302         1561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFZC010000302 DAZFZC010000000
VERSION     DAZFZC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.312"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..343)
                     /locus_tag="ACHKXP_005368"
                     /pseudo
     CDS             complement(1..343)
                     /locus_tag="ACHKXP_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(546..1559)
                     /gene="intI1"
                     /locus_tag="ACHKXP_005369"
     CDS             complement(546..1559)
                     /gene="intI1"
                     /locus_tag="ACHKXP_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO7650130.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
BASE COUNT          317 a          489 c          479 g          276 t
ORIGIN      
        1 acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg ctggcccgcg
       61 cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg gccccttccg
      121 gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg gtgctcggtt
      181 cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag gccgccgccg
      241 gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc cgcaaccacg
      301 caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt ttcagaagac
      361 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatccatcag
      421 gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg caaccggccg
      481 ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga ttgacttgcg
      541 ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg cactccggca
      601 ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga atggccgagc
      661 agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc gaacgagtgg
      721 cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc acgtttgaag
      781 gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct ccgtggatcg
      841 gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc ggcgcgcgga
      901 tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc ctggtccttc
      961 agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa gctctcgggt
     1021 aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg
     1081 aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc cgttccatac
     1141 agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg aaccacttca
     1201 tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat ctcctgaagc
     1261 cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga
     1321 cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact
     1381 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     1441 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     1501 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     1561 g
//
LOCUS       DAZFZC010000303         1551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFZC010000303 DAZFZC010000000
VERSION     DAZFZC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.313"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            477..683
                     /locus_tag="ACHKXP_005370"
     CDS             477..683
                     /locus_tag="ACHKXP_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7650131.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(939..1211)
                     /locus_tag="ACHKXP_005371"
     CDS             complement(939..1211)
                     /locus_tag="ACHKXP_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650132.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1371..>1551
                     /locus_tag="ACHKXP_005372"
     CDS             1371..>1551
                     /locus_tag="ACHKXP_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7650133.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDG"
BASE COUNT          419 a          286 c          396 g          450 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagag ccagcgactg acctgaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgctt tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcagaaggat agttggattt
      241 cacatgatac ttatgcctgg cggtatattt tctgacagac agtgacgggt gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg agttggccgt cagtgggtga cactggcggt
      421 ttttttgttt ttctttactt tcattttctg tcggcggtga cggagacata catcagatgg
      481 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt ggggacggga tactggttac
      541 tgcagctgct ggacaaagtc tctccgtccc agtgggtggc aataggtgtg ctgggaagtc
      601 tgctgtttgg cctgctgacg tatctgacta acctgtattt caaaatcaga gaggaccgtc
      661 gtaaggtggc gcggggagag tagtcgatga ataaacaata cgaactggtt gtaaaatgaa
      721 tatttctaac tgaaaaaacg ttccatgagg taagaaaagg tcacaggcaa tcaataacag
      781 gacgtgatga aagacccttg catttgtgcg ctttctcttt agatagcagc agatactgaa
      841 aatctgagtt gtcggggagt cagggataca gctgtgcaag agttggtcat tgtgattcca
      901 ttgaaatcct gtatgccatg aagggcagga ttttatggct acctgagctt tggtgatagt
      961 aagttgaaaa ttcgcatttt ttgctgacat gcgtaacgag aatcccataa gcagggagga
     1021 cttaattctt cattaaccca tgcgttgata ttatgtttca gccgttgaag catcagcggt
     1081 gttaatgttg tggtaataat atccagcgtt ttatgtgaga tcttaccgta agggtctgca
     1141 agaatgctgc ttgttgcttc gttattatct gccatcagaa gaagtaactc tgatttaacg
     1201 ttttctgtca ttagttgtaa aaatcttctg cgcaaacttt ctttactgtt catttatatg
     1261 gcttcatttg ttgtaatctg ctgcgtctca agggatatgt ttatgagagc gaccatgagt
     1321 gttggattat atacctaaca tatcaaggga ttagaaatcg ataaatcccc atgaacgaaa
     1381 aaataaaata cggcctgtcg gctgccgttc tggcgctgat tggtgcaggt gcttctgcgc
     1441 ctgaaatcct cgaccagttt ctggatgaaa aggaaggtaa ccacaccacg gcataccgtg
     1501 atggtgcggg gatctggacc atctgccgtg gtgccattct ggtggatggt a
//
LOCUS       DAZFZC010000304         1541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFZC010000304 DAZFZC010000000
VERSION     DAZFZC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.314"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..444
                     /gene="tnpA"
                     /locus_tag="ACHKXP_005373"
     CDS             <1..444
                     /gene="tnpA"
                     /locus_tag="ACHKXP_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO7650134.1"
                     /translation="KKELTEPQISEGTWRKERRASYSREFKVRLAKQALQPGAVVARI
                     AREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKPD
                     ECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKGGTR"
     gene            441..788
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005374"
     CDS             441..788
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7650135.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            808..1541
                     /locus_tag="ACHKXP_005375"
                     /pseudo
     CDS             808..1541
                     /locus_tag="ACHKXP_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          401 a          424 c          431 g          285 t
ORIGIN      
        1 aagaaggaat taacagagcc tcaaatcagc gaaggcacat ggcgaaaaga acggcgagcc
       61 agctacagcc gtgaatttaa ggtccgtctg gctaagcagg cattacagcc cggtgctgtt
      121 gttgcccgga tcgccagaga gcacggtatc aatgataacc tgctgtttaa atggaaaagc
      181 cagtacgagg acggcttact gagcgatgat gacatacagg aatgcatgcc tgtcccggtg
      241 gctctgactg atacgccaga gccgaccaga ccagttacaa atcccttctg gcgtaacaag
      301 cctgatgagt gccctgagag tgatcccgga aacgtcccac ggtgcgagct gcatcttaaa
      361 tcaggtgtgg taaaactgtt tgaccctctc actccggaaa tgttacgggc gctaatccgc
      421 gaaatgaaag gaggtacccg atgataacgc tgccaaccgg taccagaatc tggatcatcg
      481 ctggcatcac agatatgcgt tgtggcttca acggcctggc ttcgaaggtg cagaacacgc
      541 tgaaagatga cccgttctcc gggcatatct tcgtcttccg gggccgcagt ggcaaaatgg
      601 tgaaaatact gtgggccgat cgtgacgggt tatgcctgtt cgccaaacgc ctggaacggg
      661 gccgcttcgt ctggccggta acccgggaag ggaaagtgca cctgacgcca gctcagttat
      721 ccatgctact ggaggggatc gcgtggcaac atcccaaacg gacagaacgg cctggcatcc
      781 ggatataacc cgtgataaaa caggggaatg aacaacacac tccccgacga catcgagcaa
      841 ctgaaggccc tgctgatcgc acagcaggct gttatcgtct gtctggtgaa ataaccggct
      901 atgcccgcga gatcagctca ctcagagcgc tggtcgctaa actgcagaga atgttgttcg
      961 gtcgcagcag cgagaaaagc cgcgagaaga tagaaaagaa gatcgcacgg gcagaaacgc
     1021 gtataaccga gctccagaac aggcttggtg aggcgcagtt gcaactcacc tcaatggccg
     1081 gagagacagc gccgaaaaca tcagactctc ccgtccgcaa agcacttccg gcaacacttc
     1141 cccgtgacag gcaggttatc tccccggcag aaaccgaatg ccccgtctgc agcggcaaac
     1201 tgaaaccgct gggagaaagc atctctgaac aactggatat catcaacacc gcgttcaggg
     1261 taatcgaaac ggttcgccca aaactggcct gcagccggtg cgactgtata gttcaggctc
     1321 cgcagccacc aaaacccatc gagcgcagtt acgccagtcc ggctctgctg gcccgcataa
     1381 tcatggctaa gttcgccgag catctgccgc tgtaccgtca gtcggaaatc tatgcccgcc
     1441 agggcgtgga gctgcaccgc aatacgatgg ggcgctgggt tgacatcatg ggagagcagc
     1501 ttcgcccgct gtatgatgaa ctgaagcact atgtgctgat g
//
LOCUS       DAZFZC010000305         1533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFZC010000305 DAZFZC010000000
VERSION     DAZFZC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.315"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..189)
                     /locus_tag="ACHKXP_005376"
     CDS             complement(<1..189)
                     /locus_tag="ACHKXP_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFO7650136.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISNSV"
     gene            complement(189..644)
                     /gene="sepD"
                     /locus_tag="ACHKXP_005377"
     CDS             complement(189..644)
                     /gene="sepD"
                     /locus_tag="ACHKXP_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO7650137.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPTHLFSDIGRIV"
     gene            complement(650..1222)
                     /gene="escJ"
                     /locus_tag="ACHKXP_005378"
     CDS             complement(650..1222)
                     /gene="escJ"
                     /locus_tag="ACHKXP_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO7650138.1"
                     /translation="MKKYIKNAFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1225..>1533)
                     /gene="sctI"
                     /locus_tag="ACHKXP_005379"
     CDS             complement(1225..>1533)
                     /gene="sctI"
                     /locus_tag="ACHKXP_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO7650139.1"
                     /translation="DDHAPLAPTPSAAGAAQFLDQLLPKTAGVSSPEQVLIEEIKKRH
                     LATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANVNVDVVSKLASLLSTSVTKLVSMQ
                     "
BASE COUNT          445 a          281 c          292 g          515 t
ORIGIN      
        1 cacagaatta ctgatgaaaa cagggatgct ataattagcg gcaaaatcat tgaggattgc
       61 acgtaccgga gagctttttg taataataaa atactcgttc ttgcctaatc ttttttccag
      121 agaggacggg gcagcctgag cactgcagca aaatagtgct gtaaaaataa aaaaacttat
      181 ttttttcatt acacaattcg tcctatatca gaaaacaaat gtgttggagg catcgttgta
      241 tctgatgtag atgcttgctc taacttatca aattgcttgt caagcaaaga ggcgagtgtg
      301 tgttgcatta ttgcttttga catgtttgcg cgatgattat tatctttatt aaacagttgg
      361 caaagatacc attttttatt agattgaaaa atgcccattt gtttatattc tttactaacg
      421 gccagaatat tacagagtct gatgaaggta tctgttggaa cctggcgaaa atcgccatta
      481 agctcgatat acagaaccca gtaacttcca aagtcgtgta agaaggcatt tttttgatag
      541 atatgaaaag taagatgctt tggtgaacct ttgacatctt ttacaaaatc aagagccgtt
      601 agccagtcac tatcattctt aactatgcca ttattattgt tcatacatat tacccgtcct
      661 gtcctgagga tgacttgata acgacactaa tattttctag tttgagatcg tctacgctat
      721 tcttaactaa gttttttatc tgaatgactg atggtgctaa gttaacctct ggcgagctaa
      781 tgactaaaac agccgctgag gatggttggc tctcgttatt gttaacattt aatgagacgc
      841 tacaatcgat gacaccgggg attttactta acaacctttc gatatcttgt tctttgagat
      901 agtttatttt tgcattttct tgtgatggcg atgccactag ctgactgggc ggaaaaataa
      961 cttcaatatc tgcaaatttc tttttcggga agccattgtt atttaaaatg gttatcgcac
     1021 ggacaaaatc ctctttttcc acacttattg ttacatttcc acttttgtcc atttctttag
     1081 atacattgac atcattggat aacaacaatg cttgcatctg attggcttct ttttccgtta
     1141 atcctgtata caattgctct ttacacgcaa ctgtcaaaca aataaaaaca aacaaaaagg
     1201 cgtttttaat gtattttttc atttttattg cattgaaacc agttttgtaa ccgatgttga
     1261 aagaagactt gctaacttgg atacgacatc aacattgacg tttgcgttga gcacattctt
     1321 ttgtaatgtg agcacatctt ctggcgagag cccacctgca gatagtgcat cgaaactgag
     1381 atcggtgttc attgttgcaa gatgtctctt tttaatttct tcaattaaaa cttgctctgg
     1441 agaagatact ccagctgttt taggaagtaa ttgatcaaga aactgagccg ctccagccgc
     1501 agagggcgtt ggtgctaacg gggcatggtc atc
//
LOCUS       DAZFZC010000306         1526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFZC010000306 DAZFZC010000000
VERSION     DAZFZC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.316"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..1006)
                     /locus_tag="ACHKXP_005380"
     CDS             complement(146..1006)
                     /locus_tag="ACHKXP_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO7650140.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1189..>1526)
                     /locus_tag="ACHKXP_005381"
     CDS             complement(1189..>1526)
                     /locus_tag="ACHKXP_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7650141.1"
                     /translation="DMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAE
                     RRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVY
                     KILEDERAS"
BASE COUNT          397 a          380 c          336 g          413 t
ORIGIN      
        1 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
       61 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
      121 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
      181 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
      241 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
      301 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
      361 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
      421 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
      481 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
      541 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
      601 gtaagttggc agcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
      661 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
      721 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcac
      781 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
      841 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
      901 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
      961 ccgcaaaaaa gggaataagg gcgacacgaa aatgttgaat actcatactc ttcctttttc
     1021 aatattattg aagcatttac cagggttatt gtctcatgag cggatacata tttgaatgta
     1081 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     1141 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     1201 ttcgtcttca agaattttat aaaccgtgga gcgggcaata ctgagctgat gagcaatttc
     1261 cgttgcacca gtgcccttct gatgaagcgt cagcacgacg ttcctgtcca cggtacgcct
     1321 gcggccaaat ttgattcctt tcagctttgc ttcctgtcgg ccctcattcg tgcgttctag
     1381 gatcctccgg cgttcagcct gtgccacagc cgacaggatg gtgaccacca tttgccccat
     1441 atcaccgtcg gtactgatcc cgtcatcaat gaaccggact gccacgccct gagcgtcaaa
     1501 ttcctttatc agttggatca tatcgg
//
LOCUS       DAZFZC010000307         1510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFZC010000307 DAZFZC010000000
VERSION     DAZFZC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.317"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..525
                     /gene="espL2"
                     /locus_tag="ACHKXP_005382"
     CDS             <1..525
                     /gene="espL2"
                     /locus_tag="ACHKXP_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO7650142.1"
                     /translation="DAIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGLLLAYQNGDI
                     NTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISHRHDHVVKLYGKLFKKLDT
                     SPYKMSIILALAIDCERNNANIIIDSEYKSNKAVKEYVEILKEFNICPEKVAEYLSEF
                     SGKHFLDVYNYYSN"
     gene            707..892
                     /locus_tag="ACHKXP_005383"
     CDS             707..892
                     /locus_tag="ACHKXP_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7650143.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            1133..>1510
                     /gene="nleB"
                     /locus_tag="ACHKXP_005384"
     CDS             1133..>1510
                     /gene="nleB"
                     /locus_tag="ACHKXP_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO7650144.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSM"
BASE COUNT          540 a          236 c          280 g          454 t
ORIGIN      
        1 gacgcaataa aggcatattg tggcgtactt ggcaatagta atttgaaaag aggtgagata
       61 atcaggatgc ttgaggcgag aaattatgat ggtgcaccag gattacttct tgcgtatcaa
      121 aatggagata ttaatacgat tcagtctttt tttgactcac taataatgtt agatatatca
      181 aaggatttta tcgaagagtt attgactgca aaacattatg attttacggg gttatcatta
      241 gcgatcagtc atagacatga tcatgttgtc aaattatacg gaaagttatt taaaaaactt
      301 gataccagtc catataaaat gtcaattatc ctagcattag caattgattg cgagagaaat
      361 aatgcaaaca taattattga tagtgagtac aagtcaaata aggcagtaaa ggaatatgta
      421 gaaattttaa aagaattcaa tatatgccca gaaaaggtag cagaatacct aagcgagttc
      481 agcggaaaac atttcctcga tgtatataat tattattcca attgaaaaaa atataaagta
      541 tctatcacgg cactctcagt tgagtatcac aggcacatgt attttagccc cctgagtcta
      601 cagacaacca atattactta aataagtgat aatcttaatg ctgtttttta gaacagatga
      661 ttgatatttt tgggccggag aaacgcagac aggctttgtt gaataaatcg agcttttgct
      721 gagtttaagg atcagatcac tcattttcct gcaaaagctc attgggcgaa acaatctgac
      781 tcagagaacc cggattaaac gtctggcccg cagaaccatc tatttctcac gcactgtagt
      841 acgtaataaa aaagtcatcg gtacttttat caaaaaacat atattctatt aattattgcc
      901 atcacctttt tcttattgac aacattactc tgctgattgt taaaatctaa cctgtattca
      961 gggttacgat tctatacaat agaacaggga ggggaaagga tgtcatcgtt aatgagactt
     1021 tttgttaatg tcaacaatgc tatgaattta ttttagaaaa acaattaagc tgacgtagtg
     1081 ttccgtctaa atgacggggc gatattaaca tgattagaac aagaggaatt ttatgttatc
     1141 ttcattaaat gtccttcaat ccagcttcag aggaaagaca gccttatcaa atagtacact
     1201 tctccagaaa gtttcttttg ctggaaaaga atatcctctg gaacctattg atgaaaaaac
     1261 ccctattctt tttcagtggt ttgaagcaag gccagagcga tacgaaaaag gagaagtacc
     1321 aatattgaat accaaagaac atccgtattt gagcaatatt ataaatgctg caaaaataga
     1381 aaatgagcgt ataatcggtg tgctggtaga tggaaatttt acttatgaac aaaaaaagga
     1441 atttctcagt cttgaaaatg aatatcaaaa tataaaaata atctaccgag cagatgtgga
     1501 tttcagcatg
//
LOCUS       DAZFZC010000308         1504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFZC010000308 DAZFZC010000000
VERSION     DAZFZC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.318"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..383
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005385"
     CDS             33..383
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7650145.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            414..874
                     /locus_tag="ACHKXP_005386"
                     /pseudo
     CDS             414..874
                     /locus_tag="ACHKXP_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(917..>1504)
                     /locus_tag="ACHKXP_005387"
     CDS             complement(917..>1504)
                     /locus_tag="ACHKXP_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
                     /protein_id="HFO7650146.1"
                     /translation="GKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQG
                     FVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSL
                     AYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWV
                     DWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          356 a          407 c          398 g          343 t
ORIGIN      
        1 atgttaatcc gcgaactgag cgggagggga agatgatttc actcccatca ggtacccgta
       61 tctggctcgt tgccggcgtt accgatatgt gtaaatcctt caacggactg ggagaacagg
      121 tacaacatgt gctgaatgat aatcccttct ccggtcacct gtttatcttc cgtggccgac
      181 ggggggacac cgttaaaatt ctttgggctg atgctgatgg tctgtgcctg ttcaccaaac
      241 gcctggagga aggccagttt atctggcctg cggtgcgtga cggtaaggta tccattaccc
      301 gctcgcaact ggcaatgctc ctcgataagc tggactggcg tcagccaaaa acatcccgcc
      361 ttaacgccct gacaatgttg taaaaaacac ctggccgcat tataaaaacg gccatgagtc
      421 agaaatacct cattcgcatt gcagaactgg aaagccagct ccgtcagaaa gaccagcaac
      481 tgagtctggt tgaagagacg gaggccttcc tgcactggca cgtgccgaag aaaagatcga
      541 agaagatgaa cgggaaatag aacatctgcg ggctcagata gaaaaactgc gccggatgct
      601 gttcggtacc cgttctgaaa aactgcgtcg tgaagttgaa caggctgagg ccctgctgaa
      661 acaacgcgaa caggacagcg atcgttacag tgggcgggaa gacgatccac aggttccccg
      721 ccagttgcga cagtcgcgcc atcgtcgtcc gttaccggca caccttcccc gtgaaataca
      781 gcgtctggaa tctgaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg
      841 ggaagtcagt gcagaacaac tggaactggt gagctgaacc gccccgggtt tcctggagag
      901 tgttttatct gtgaactcag gctgccagat catcgtttcc gatggaagca taataagctt
      961 tttctgcttc tgccggagga gtatggccca gcctttccag caatcgtcga ttgttatacc
     1021 agtccaccca cgtgagtgtg gccagttcca cttctgcacg gtttttccag ctcttacggt
     1081 gtattacctc cgctttgtaa agaccattga tgctctccgc catcgcgttg tcatacgagt
     1141 cgcctgtact tcctgttgat gccagtaatc cggcttcctt aagccgctgt gtgtaggcca
     1201 gcgatacata ctgagaacct ttatcactgt gatggaccgt gccggacggt cgacgggccc
     1261 ataacgcctg ctccagtgca tccagcacga atgtcgtctc catggacgat gagacccgcc
     1321 accccacaat gtatccggca aacacatcaa tgatgaacgc cacatagacg aagccctgcc
     1381 atgtgctgac gtaagtaaaa tcagccaccc acagctggtc aggtcgttct gccacgaact
     1441 gacggtttac gcggtcgcct gcggcaacgg ctttccggct gatggtcgta cggacctttt
     1501 tacc
//
LOCUS       DAZFZC010000309         1500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFZC010000309 DAZFZC010000000
VERSION     DAZFZC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.319"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            138..>1500
                     /gene="ygeH"
                     /locus_tag="ACHKXP_005388"
     CDS             138..>1500
                     /gene="ygeH"
                     /locus_tag="ACHKXP_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO7650147.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTR"
BASE COUNT          545 a          259 c          257 g          439 t
ORIGIN      
        1 agataaatta gtgaacacaa ccatagatat gtcgaatgta atttcctgta gcgttaatga
       61 cacataaata tgaatagcca taatttctat tgcttaagca ggatgcaaga aaccaatttt
      121 ttcatagagg ttaactaatg gacttagaaa ataaattctc atatcatttt cttgagggat
      181 taacgctcac ggaagatgga attcttactc aaggaaatga gcaagtttat attccacaga
      241 aagagttagg tgtattaata gtattacttg aatccgctgg tcatgttgta ctgaaagata
      301 tgatcatcga atcagtatgg aaaaatatta ttgttagtga cgagtccctg acaagatgta
      361 tctattcttt gcgctgcatt tttgaaaaaa ttggctatga tcgttgcata gaaacaatct
      421 accggaaagg ttatcgtttc agcgggcagg ttttcaaaac taaaattaat gaagataata
      481 cttcagacta ttccatagct atattccctt tcactacttc attgaataca ctggatccat
      541 taatacttaa tcaggaatta gtgcaaatca tttcaaataa aaaaatcgat ggtctctata
      601 cctatccgat ggctgcgaca aatttttgta atgatcacat atctcaaaat tcattcttga
      661 gcagattcaa gccagattat ttcgttacag gaagaataaa ccagaataat gcagtgaaca
      721 ctttatacat tgagttgatc gacgctaaaa accttttcct catcgccagt aatcatctcc
      781 ctgttgatga actacataat acatcacaat ttattataga taatatcctt caaacggtac
      841 ataaaccaga acgatctgta agattagcta agcaggacca aggatataag aatcattatt
      901 tatcagatga aatgttagcc ggaaagaaag aactttacga cttcacccct gaaagcattt
      961 acagggccat gactatattt gatagattac aaaataaaag tgatatacag acgctaaaaa
     1021 cagaatgtta ttgccttcta gcggaatgcc atatgtcttt ggcacttcat ggaaaaagtg
     1081 aacttgaact tgctgctcaa aaagcattag agcttttaga ttatgtatca gacataacca
     1141 ctgtcgatgg aaaaatttta gctattatgg gactgataac tggtctgtct ggacaagcaa
     1201 aagtatctca tatcttattt gaacaggcta agatacactc aactgatata gcctctctct
     1261 actactatag ggcactagtc cactttcata atgaaaaaat tgaagaggca aggatttgta
     1321 tagacaaatc actacaactc gaacccagaa gacgaaaagc agttgtgata aaagaatgtg
     1381 tagatatgta tgtgcctaac ccgctcaaaa acaacattaa actctactat aaagaaactg
     1441 agagtgaaag ccatcgagtt ataattgaca acattttgaa attaaagcag ctgacgagaa
//
LOCUS       DAZFZC010000310         1488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFZC010000310 DAZFZC010000000
VERSION     DAZFZC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.320"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            80..580
                     /locus_tag="ACHKXP_005389"
     CDS             80..580
                     /locus_tag="ACHKXP_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7650148.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEY
                     QHRNLKPNTEPVNILLSNNFIRINKNIRLSDLTHFLISNLHTQNVYSTQEPLTHDDID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            582..857
                     /locus_tag="ACHKXP_005390"
     CDS             582..857
                     /locus_tag="ACHKXP_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO7650149.1"
                     /translation="MSLVGYNSKIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVIS"
     gene            complement(937..1488)
                     /locus_tag="ACHKXP_005391"
                     /pseudo
     CDS             complement(937..1488)
                     /locus_tag="ACHKXP_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          484 a          266 c          234 g          504 t
ORIGIN      
        1 agcaaatata acaattactc ttattttaac ttttacacgt aatatttacc ctaaatgatt
       61 ttagagtaca ggttggcata tgcaagtctt ttccagtgat gtctacttca ctgtaggcac
      121 taatgcatta ttagcatccc aaaaagaata ttacagcgat ttagttgcgt tggttgatct
      181 tgggcattcg ttcgttgtta ttgatgagta tcaacatcga aatctcaaac caaatacaga
      241 acctgtaaac atccttttaa gcaacaattt catcaggatc aataagaata ttagattatc
      301 tgatttaacg catttcctga tttcgaatct tcatacacaa aatgtgtatt ccacacaaga
      361 accacttacc catgatgata tagatatcct gcggttatgt gtttcttact cgttaaaaca
      421 aatagccatt attaaaggaa ttgattataa aacagtatca tatcacaaaa taagagccct
      481 aaataaactt aacataaaag gtactgttga gttgtttata gcactctgtg aatgggataa
      541 acattatttt aaactccaat cttgcgtaag agaaagttga tatgtcatta gtaggatata
      601 atagtaaaat cataataacc atcgcgctga tatcgttgct ctctgcatgc accagagaaa
      661 attcattaaa ggagaatcaa aataaaagaa gtggcgaatc ccaagcgata gtagatgcaa
      721 gacagttctt tagcgaacac ccggaatata tatcttcagc agaactggag caaaagctat
      781 atcgcgaatt tgcacaggtt actacagttc cagcttataa agagatgagt atgtatcaat
      841 tgcttgtcat ttcataagat cgtttaactc attaaataat gaatctacac ggtttttgcg
      901 tgtagattct tataaatttc tgactacgac agatgtttaa ttttttaaat tatcgtcttt
      961 attctcgatt tctttctctt caccttcaat gaatttatct tctgaagaga gctgttcatc
     1021 tggaacaggt tgtccagcat tctccacatc ttcaagccac agcagaagac gtaatatttc
     1081 atctatattt tcgaagctaa cataatcgta gcgacgatgg gtagcataaa tttttcgtgc
     1141 taatttttta tctgtgataa ttggtattcc gatttccttt gcatatttcc ttacggctaa
     1201 ggctacctca ttagtttctc ttacagaaat caatggaata ggtgaaagat gtggcttaaa
     1261 gtatatccct attgcaatgt gagtagggtt ggcaatcatc aaacgactat tactgacatc
     1321 agacttcaat tgctcagaaa gaatttcctg atgcgctctc tgcgtttaga cttaatttct
     1381 ggatttcctt cttgctcctt gtattctctt ttaacttctt gtttatccat tttcatatct
     1441 ttcataaata aaaaatattc agcgataaaa tcaaaaatta agacaatt
//
LOCUS       DAZFZC010000311         1485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFZC010000311 DAZFZC010000000
VERSION     DAZFZC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.321"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..505
                     /gene="yhdJ"
                     /locus_tag="ACHKXP_005392"
     CDS             <1..505
                     /gene="yhdJ"
                     /locus_tag="ACHKXP_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7650150.1"
                     /translation="EPILMMVKDAKNYTFNGDAILVEAKTGSQRALIDYRKNPPQPYN
                     HQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIVLDPFAGSFTTG
                     AVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTGNRSKRCRVSEV
                     DPDLIAK"
     gene            589..768
                     /locus_tag="ACHKXP_005393"
     CDS             589..768
                     /locus_tag="ACHKXP_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO7650151.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(771..1433)
                     /gene="acrS"
                     /locus_tag="ACHKXP_005394"
     CDS             complement(771..1433)
                     /gene="acrS"
                     /locus_tag="ACHKXP_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO7650152.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          410 a          338 c          327 g          410 t
ORIGIN      
        1 cgaacccatc ctgatgatgg tgaaagacgc aaagaactac acattcaacg gtgatgctat
       61 tctggtagaa gccaaaaccg gatcgcagcg cgcgttgatc gattatcgca aaaatcctcc
      121 acagccatac aatcatcaaa aagtaccggg taacgtctgg gattttccgc gcgtgcgtta
      181 tttaatggat gaatatgaga accacccgac gcaaaaaccg gaagccttac tgaaacgcat
      241 tattctcgcc tcttccaacc caggcgatat cgttctcgac ccgtttgccg gtagctttac
      301 taccggtgcc gtagccatcg ccagcggacg aaaattcatt ggtattgaga tcaacagcga
      361 gtacatcaaa atggggcttc gacggctgga tgtcgcgtcg cattactccg cggaagaact
      421 ggcgaaagtg aaaaaaagaa agacgggcaa ccggtcaaaa cgatgccggg ttagcgaagt
      481 tgaccccgat ctcattgcaa agtaaaggaa gtgtaagcct ggttttcaga ttattcattt
      541 cgtgtatatt tcctgccaga cttggttaaa catgcacagg ctcaggtaat gattcgcaag
      601 tattggtggc tcgtcgtttt cgctgtcttc gtttttctgt ttgatacttt actgatgcag
      661 tggattgaac tgctggcaac agaaacagac aaatgccgta atatgaactc agttaatcca
      721 ctaaaactgg ttaactgtga cgaactgaat tttcaggaca gaatgtgaat ttacatgaca
      781 cttaattcat tcgtttgatg aattaatttc gttatgtttt catctggcat gaacattctt
      841 aatacgttat cgaccagcgc gggtgcttgt ttataaagat cataacccgc catattcatt
      901 aaccagtttt gaacaattcc gctgaaggca ccatcaataa taatcatcac aacatctaaa
      961 tcgaggttat ttgctacaca accttgttgc tgacacgcct gcaatacttc gcggagagtc
     1021 tgcggattaa agcccatctt ttcgcgtatc actccctcgg ccagcatctc atcattaaat
     1081 tcacatttgt gatataagat tttcagcaac gcctgctggc ggggaatttt ggcaatatat
     1141 tgcaagccga caatcaattt ttcacgcaat tgttgaaacg ggtcatgctc taatccagcc
     1201 gtcaagtgtt cctggattaa ctcccgcaat gaaggctgtt gcaaccacat ctcattaaac
     1261 agttgggtct tgttctcgaa gtgccagtag atagcgccac gcgtaacgtt agcggcatcg
     1321 gcaatgtcgt tgagcgtcgt cttgcttacg ccatgttgcg caaactgggc gatggcagtt
     1381 tcaatcagtt cttgccgggt cttcagagct tcggctttgg ttctttttgc catgattaat
     1441 tattcaggaa ataaatatat tcgacacagg gtgagaaaat agcga
//
LOCUS       DAZFZC010000312         1431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFZC010000312 DAZFZC010000000
VERSION     DAZFZC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.322"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..230)
                     /locus_tag="ACHKXP_005395"
     CDS             complement(<1..230)
                     /locus_tag="ACHKXP_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7650153.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKEL"
     gene            285..1412
                     /locus_tag="ACHKXP_005396"
                     /pseudo
     CDS             285..1412
                     /locus_tag="ACHKXP_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc47 family transposase"
BASE COUNT          353 a          380 c          403 g          295 t
ORIGIN      
        1 tccagctctt tcagacgctg acgttcagcg gtggtgagcc ctccatcacc gcccccggta
       61 tcccgctcat gctggcgaac ccagacacgc agagtctccg gcgtacagcc aatctttgga
      121 gcaatggaac aaattgtcgc ccattgtgag tcatattcgc cctgactttc cagaaccata
      181 cggactgccc gttgacggac ttcaggggaa aaacgagtat ttttagtcat cctgtttacc
      241 tctttctcag gaagtttagt ctccaggatt cccggggcgg ttcaagcagc gccctgaaag
      301 tgatccgcac agaacgggta aaaaaagcgt gtacaaaatg tgactgcatc gttgaagcac
      361 cggcgccgtc ccgcccgata gagcgtggta tcgcgggccc cggattactt gcccgcgtgt
      421 taacgggaaa atactgcgaa cacctgccac tgtatcgtca gagtgaaatc tttgcccgac
      481 agggtgtcga actgagccgt gcattactct ccaactgggt tgacgcgtgc tgccagttaa
      541 tgacgccgct gaatgatgcc ctgtaccgtt atgtgatgaa tacccgcaag cttcacactg
      601 acgacacacc ggtaaaggta ctggcaccgg gcctgaaaaa gacgaaaaca gggcgcatct
      661 ggacgtatgt ccgggatgat cgcaatgcgg gttcgtcatc tcctccggcg gtctggttcg
      721 cgtactcacc gaaccggcag gggaaacacc cggagcaaca cctccgcccc ttccggggta
      781 tcctgcaggc ggatgcgttc acaggttatg acaggctgtt cagtgcagaa cgtgaaggtg
      841 gtgcgctgac agaagttgcg tgctgggccc atgcccggag aaaaatccac gatgtataca
      901 tcagcagcaa aagtgcgacg gcagaagaag ccctgaagcg aatcagtgaa ctgtacgcca
      961 tcgaggatga aatacgggga ttaccagagt cagagcgtct tgcagtcaga cagcagcgaa
     1021 gcaaagcgtt actgacgtcg ctgcatgaat ggatgatgga gaagaatggc acgctgtcga
     1081 aaaaatccag actgggcgaa gcgttcagct atgtactgaa tcagtgggac gccctctgtt
     1141 attacagtga tgacggactg gcggagacgg acaataacac agcggaaaga gcgcttcgtg
     1201 cagtctgtct tggaaagaaa aattacgtgt tcttcggtag cgatcacggc ggcgagcgtg
     1261 gtgcactgct atacgggctg atcggcacct gccgtctgaa cggtatcgat ccggaagcgt
     1321 atctgcgcca tattctgagc gtactgccgg aatggccctc caaccgggtt gatgaactcc
     1381 tgccatggaa cgtagtactc accaataaat aagcgtcaat acggtgctcc g
//
LOCUS       DAZFZC010000313         1410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFZC010000313 DAZFZC010000000
VERSION     DAZFZC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.323"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(418..792)
                     /locus_tag="ACHKXP_005397"
     CDS             complement(418..792)
                     /locus_tag="ACHKXP_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650154.1"
                     /translation="MEFKDLPMQFQEMAANIVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLCEEKQLSDNQNLHEKYFLELMNIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(815..1033)
                     /gene="ydfC"
                     /locus_tag="ACHKXP_005398"
     CDS             complement(815..1033)
                     /gene="ydfC"
                     /locus_tag="ACHKXP_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO7650155.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1063..1191)
                     /gene="ydfB"
                     /locus_tag="ACHKXP_005399"
     CDS             complement(1063..1191)
                     /gene="ydfB"
                     /locus_tag="ACHKXP_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO7650156.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFNEFKQALSSSAKSNG"
     gene            complement(1193..1348)
                     /locus_tag="ACHKXP_005400"
     CDS             complement(1193..1348)
                     /locus_tag="ACHKXP_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7650157.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
BASE COUNT          342 a          339 c          302 g          427 t
ORIGIN      
        1 ttttaactgt tccgcgtgcg ctcagccgca ttcaccgcat cacaaaattc actttaaaaa
       61 gggcggcaga gcagtcacgg agtaaaactg ataccgccaa acgtcaccag aaaattgata
      121 acagagggcg ttgcagcggg gttgtcactt aagcgtatgg tcaacctgac aacccggtgt
      181 cctcaacggg gaaggaataa ccccgccata cttaccgccg cgccatttcg cggattgcca
      241 caaccggaag cgcacgatcg aattaaattt aacgacgacc tatacagaga gactaacttc
      301 gccgggcgct ttcgtgttat gccctgactt ttcagggata tatccttttc agtaaactgt
      361 cagtgccgga ttcttatccg tgtccggcgc acgcactcta cctcacctgt gaataaatta
      421 atgattaatt gatattttgt tgtttgattc aactttccca tcggatgtgt gatgctttaa
      481 atcacaggaa ttaatactgc ttgctgtaaa atgattttca aggggagcta ttcgaatccc
      541 tttctttttc attaacaagc caaatccttt attaatgatg ttcattaatt ccaggaagta
      601 tttttcatgt aaattctggt tatcagagag ctgcttctct tcgcacagac cgataaaggc
      661 acgacgcacg ttaccggata tagtatcgat ggtttctttt tctacggtac tcaggtcaag
      721 agtcgccagt tgggaacgaa ctatattcgc tgccatttcc tggaattgca ttggtaaatc
      781 tttaaattcc attattagcc tcgttggtta gctattaacg cgggtatgta atcattctgg
      841 caatgcttaa tgccgctgct ttttccagat tagtgatatc ctgctccaga gcggacagat
      901 tttcagcctg cttagccctg gcttcattgg cccatttcag atcctgcgct gcattaattt
      961 tctggtgcat ccactcataa agttcatcat cggtatagtc tggcgcgatg atgacgggtt
     1021 ctcgtttctg catactgatt cctcgcggtg ctgtttcgct tatcagccgt tagattttgc
     1081 cgaactggaa agcgcctgtt taaattcgtt gaagctgaga gcttcttcgc cttcggcaag
     1141 accttcgaag tattcttcgt aagccttttc catgattgtg tcgaaatcca tatcactcac
     1201 ctgagtttct ttccagccag cgacgggcac cattttcggt tttaaacgtt ttgcttttgg
     1261 tatacgtcat cgcggtgaac gtaccgtcct ggttggggaa cacgccacat accagagatt
     1321 cgctgttgcc aagatcgata gtatccatgc tgacctcatt tccccttaac gccggggtag
     1381 cggaacaaaa acctgctgca tagttattaa
//
LOCUS       DAZFZC010000314         1383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFZC010000314 DAZFZC010000000
VERSION     DAZFZC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.324"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            394..1218
                     /gene="gadX"
                     /locus_tag="ACHKXP_005401"
     CDS             394..1218
                     /gene="gadX"
                     /locus_tag="ACHKXP_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO7650158.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVFVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLSNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          412 a          258 c          279 g          434 t
ORIGIN      
        1 cagaacagct ttaaacacac ctgataacat aacgttgtaa aaaccgaatg cccagccttt
       61 aaaaaaacag ctgggcattc ggttgcttat taatcgcaat aatatattgg ctgtctattc
      121 atcgtgttga taagatatat gttatatgaa tgtttattaa atgtagcgat tacctttaat
      181 gtcacgaaaa gggattattt gcttactatt aatttccctg cgttcttaaa cacgtttttg
      241 tattataaat ccagtcatcc tgcccgtaga acatcacaca ttatcatcct gttctcccgc
      301 ttcgtttaaa tttatttatc aatcaatttg acttaagagg gcggcgtgct acattaataa
      361 acagtaatat gtttatgtaa tattaagtca actatgcaat cactacatgg gaattgtcta
      421 attgcgtatg caagacataa atatattctc accatggtta atggtgaata tcgctatttt
      481 aatggcggtg acctggtttt tgcggatgca agccaaattc gagtagataa gtgtgttgaa
      541 aattttgtat tcgtgtcaag ggacacgctt tcattatttc tcccgatgct caaggaggag
      601 gcattaaatc ttcatgcaca taaaaaagtt tcttcattac tcgttcatca ctgtagtaga
      661 gatattcctg tttttcagga agttgcgcaa ctatcgcaga ataagaatct tcgctatgca
      721 gaaatgctac gtaaaagagc attaatcttt gcgttgttat ctgtttttct tgaggatgag
      781 cactttatac cgctgcttct gaacgtttta caaccgaaca tgcgaacacg agtttgtacg
      841 gttatcaata ataatatcgc ccatgagtgg acactagccc gaatcgccag cgagctgttg
      901 atgagtccaa gtctgttaaa gaaaaaattg cgcgaagaag agacatcata ttcacagttg
      961 cttactgagt gtagaatgca acgtgctttg caacttattg ttatacatgg tttttcaatt
     1021 aagcgagttg cagtatcctg tggatatcac agcgtgtcgt atttcattta cgtctttcga
     1081 aattattatg ggatgacgcc cacagagtat caggagcgat cggcgcagag attgtcgaac
     1141 cgtgactcgg cggcaagtat tgttgcgcaa gggaattttt acggcactga ccgttctgcg
     1201 gaaggaataa gattatagag ttttactcag acataaaaaa aacccggcat agggggccgg
     1261 gaagaggata gtctgccgtc tccagactaa taaaccgtta taacactccc tgttggcacg
     1321 ggaaactttg tgctctcagt aagttaaata taacttttac tggaaataag atcagccatt
     1381 ttt
//
LOCUS       DAZFZC010000315         1366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFZC010000315 DAZFZC010000000
VERSION     DAZFZC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.325"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..278)
                     /locus_tag="ACHKXP_005402"
     CDS             complement(<1..278)
                     /locus_tag="ACHKXP_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7650159.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERL"
     gene            complement(336..683)
                     /locus_tag="ACHKXP_005403"
     CDS             complement(336..683)
                     /locus_tag="ACHKXP_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7650160.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(720..>1366)
                     /locus_tag="ACHKXP_005404"
     CDS             complement(720..>1366)
                     /locus_tag="ACHKXP_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7650161.1"
                     /translation="VMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGRE
                     QLATMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAA
                     MPEMTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARA
                     VLAAGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          256 a          433 c          350 g          327 t
ORIGIN      
        1 agacgctcaa ccgcctgctg gtaattaaat tcgtgtttcg gcttgacgta gcccggacgt
       61 aacacgcggg tttcaccacc gcgatgacgc agcacttttc cttcaacaac cggggagaca
      121 taggccgcca ccggcgtttt tccggtaatt ttgtccagca tcacctcttc ggtatggaaa
      181 ttcaccgtac ggcggaaaaa cagctccaga aacagcgcac gaaatttcac tttttgttcg
      241 gtataaccga gtaactggcg ggtcgtaaac aatcccataa atcagttcct ttcattcaga
      301 aatcagtcag gccaccgcgg tggcctgata acgtgttacg gcagcgccgc gtgactcagg
      361 gcactgccgg caaaggcatt tgcctttttg tgttcatcca cactgtcagg ccagcggatt
      421 gcctccgtcg caaaggtccc cgacttgtaa tacgtcagta ccgtctctgt gccttcaagc
      481 ggcagtacca gtatgccaac cgcactaccg gctttctgtc cgtcccagac caccagtttc
      541 ccggtagctt catccagcat caggggcgtc agtgccggtg ttgccgagga aatcccgctg
      601 ctgcctgtgg cggtatgagc cggatcatta ccggcaaaaa tacgtacttc cgcacgctgt
      661 tcagtgatgg ttttcgttac catattgtaa aaacctcctg ttgatggtca gcactgactt
      721 catggcatag ccatgagcat tttcacgtcc gcatcaccgt ctgctgacgt ctgtggcacg
      781 ccaccctgta ccgctgccgg tgaatggttc gccatgatgc gttcaaacag ggcggttgtg
      841 gatgcagaga ccggttctgc cttacctgat cccgcagcca gcacagcccg ggcgctctcc
      901 acagtcattc ccgggcaggc agccagctgt tcagcctgcg cctcagcccc ttttgcctca
      961 tccagtgcca tgatctgatc acggagtgag ggtccggcat ccgcctgcgg tgaagcagcc
     1021 aggatcgggc gggctttttc caccgtcatc tccggcatcg ccgccagcgt tgccgccagt
     1081 tgttcacgac cgttcgcttc ttcacacgcc ataatgcgat cggcttcact ctgcgtggat
     1141 gccaccggct gctgcggtgc cgccgcggcc agaatcgccc gggcctgttc aacgctcatg
     1201 ccctgttgtc ctgccagcat cgtggcaagc tgttcacgtc ctttcgcttc ctggcatgtc
     1261 aggatcccca tcactcgctg gttctcctgc gcggcggctt ccgttgcagt taattgcggc
     1321 atagtgcctc ctctgacatt actgttcagc gccgtggcca tcacac
//
LOCUS       DAZFZC010000316         1350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFZC010000316 DAZFZC010000000
VERSION     DAZFZC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.326"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..162
                     /locus_tag="ACHKXP_005405"
     CDS             <1..162
                     /locus_tag="ACHKXP_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO7650162.1"
                     /translation="SDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIRITDIDTSGIFD
                     PDDMTIKAA"
     gene            235..399
                     /locus_tag="ACHKXP_005406"
     CDS             235..399
                     /locus_tag="ACHKXP_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO7650163.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            368..511
                     /gene="kil"
                     /locus_tag="ACHKXP_005407"
     CDS             368..511
                     /gene="kil"
                     /locus_tag="ACHKXP_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO7650164.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            587..883
                     /locus_tag="ACHKXP_005408"
     CDS             587..883
                     /locus_tag="ACHKXP_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7650165.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            889..>1350
                     /gene="bet"
                     /locus_tag="ACHKXP_005409"
     CDS             889..>1350
                     /gene="bet"
                     /locus_tag="ACHKXP_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7650166.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHK"
BASE COUNT          382 a          314 c          334 g          320 t
ORIGIN      
        1 agcgacttaa atgcatatgg tgttgtgtgt gagttcgact ggaatgatgg aaatggtcag
       61 gaaggatggc ctccaatgga tggtagcgaa ggaataagaa ttaccgatat cgatacatca
      121 ggaatatttg atccagatga tatgactatc aaagccgcct gagcgcggcg ttaccgcata
      181 ccaataacgc ttcactcgag gcgtttttcg ttatgtataa ataaggagca caccatgcaa
      241 tatgccattg cagggtggcc tgttgctggc tgcccttccg aatctttact tgaacgaatc
      301 acccgtaaat tacgtgacgg atggaaacgc cttatcgaca tacttaatca gccaggagtc
      361 ccgaaaaatg gatcaaacaa ttatggctat ccagactaaa ttcactatcg ccacttttat
      421 tggcgatgaa aagatgtttc gtgaggccgt cgacgcttat aaaaaatgga tattaatact
      481 gaaactgaga tcaagcaaaa gcattcacta accccctttc ctgttttcct aatcagcctg
      541 gcatttcgcg ggcgatattt tcacagccat tttcaggagt tcagccatga acgcttatta
      601 cattcaggat cgtcttgagg ctcagagctg ggcacgtcac taccagcaga tcgcccgtga
      661 agagaaagag gcagaactgg cagacgacat ggaaaaaggt cttccacagc acctgtttga
      721 atcactctgc atcgatcatt tgcaacgcca cggggccagc aaaaaagcca ttacccgtgc
      781 gtttgatgac gatgttgagt ttcaggagcg catggcagaa cacatccggt acatggttga
      841 aaccattgct caccaccagg ttgatattga ttcagaggta taaaacggat gagtacagca
      901 ctagcaacgc tggcagggaa gctggctgaa cgtgtcggca tggattctgt cgacccacag
      961 gaactgatca ccactcttcg ccagacggca tttaaaggtg atgccagcga tgcgcagttc
     1021 atcgcattac tgatcgttgc caaccagtac ggccttaatc cgtggacgaa agaaatttac
     1081 gcctttcctg ataagcagaa tggcatcgtt ccggtggtgg gcgttgatgg ctggtcccgc
     1141 atcatcaatg aaaaccagca gtttgatggc atggactttg agcaggacaa tgaatcctgt
     1201 acatgccgga tttaccgcaa ggaccgtaat catccgatct gcgttaccga gtggatggat
     1261 gaatgccgtc gcgaaccatt caaaacccgc gaaggcagag aaattacggg gccgtggcag
     1321 tcgcatccca aacggatgtt acggcataaa
//
LOCUS       DAZFZC010000317         1346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFZC010000317 DAZFZC010000000
VERSION     DAZFZC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.327"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..1180
                     /locus_tag="ACHKXP_005410"
     CDS             35..1180
                     /locus_tag="ACHKXP_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO7650167.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILVLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAEQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          462 a          278 c          242 g          364 t
ORIGIN      
        1 atcattcata ataaatgaga caggggaatc tctcatgata aacggactta ataataattc
       61 cgcatcttta gttttagatg ctgcaattag aattaattct gattttaaaa agcagtggaa
      121 tgatatgtca tgcgctgaaa aattactaaa agttcttagt tttggtttat ggaatccaac
      181 gtatactcgt agtgaaaggc aaacatttca agagttgtta accgttttag aacctgtatc
      241 tcccgcgcct aatgaattgg gcagaatata tgctaatttt gcagatggct catccttaag
      301 aatttccgtc actaacagcg aacttgttga agccgagatt cgcacaccag ataatgaaaa
      361 gattcttgtg ctcctggagt caaacgaaca aaatagatta ttacaatctt tacccatcaa
      421 tctccacatg ccatacattc aggttcatcg tgccttatct aagatggacc tgactgatca
      481 taagtcaatg cacaatctac ttagttttac gtcaaaacta tcagcaacct tgattcctca
      541 taatactcaa acagatccgc tttccgggcc tacaccattc agctctatgt ttatggatac
      601 atttcgagga ctcggcaatg caaagctttc actcaatggt gttgatatac ctgtagatgc
      661 acaaaaattg cttcgcgatg cactaggact taaagacaca cattcatcac ttgcccggaa
      721 tgttataaat aatggtattt ctcgtcatca tgcagagcaa atagcaaggg aaagcagcgg
      781 cagcgataaa cagaaagctg aagttgtgga atttttatgc caccccgaag cggcaacagc
      841 catatgctcg gctttctatc aatctttcaa tgtgccagcc ttaatgttga cacatacaag
      901 gatctctcaa gccagagaat acaatgtgga aagatcgtta gatgtaccta acgcgtgcat
      961 taacataagt atctctcaat caccagatgg aagcattcac gttgccagcc ataccgggat
     1021 tctgataatg gcgccagaag accgccccaa cgagttagga atgttgacga acaggacctc
     1081 ctatgaagtg ccacaaggtg tgaaatgtga aatcgatgag atggtaagaa cgctacaacc
     1141 aaggtatggc gcatctgaaa catacctgaa aaacatttaa aaataaaaaa atggacacca
     1201 tacactttat ttatcaaaac aaacaataac taatcacact cacaattatt aataatttaa
     1261 cctcacagca atcatacaaa acaaagatat ttatttcgca aaccatttat agcgaatgga
     1321 gaaggaatga aaagaagctt atttat
//
LOCUS       DAZFZC010000318         1334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFZC010000318 DAZFZC010000000
VERSION     DAZFZC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.328"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..1258
                     /locus_tag="ACHKXP_005411"
     CDS             26..1258
                     /locus_tag="ACHKXP_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7650168.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYQCTIEHIQKMREMYRDVTDTFYWVALPLT
                     TVNGVSQFQPEWICWESGVTWVRQPPEEAITDMTYFPFYRYAMAFEEFVPAFSSWFAG
                     NRCGVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMNPLYDWKAR
                     DIWIYNARTCAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANQSGAYFALRKRISKPAHHAWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            1243..>1334
                     /locus_tag="ACHKXP_005412"
     CDS             1243..>1334
                     /locus_tag="ACHKXP_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650169.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQAL"
BASE COUNT          323 a          315 c          360 g          336 t
ORIGIN      
        1 aattattcga cataaagcca ggaaaatgag tgatacgctt ttaaccgaaa aaattcttac
       61 cggagaaaat gttttgcgtg ccgcaatcgc ccgcattgag tggatttttg aaacatttcc
      121 gtcggtatgc ctgtcttttt caggtggaaa ggattccacc gtgcttttcc atcttgtggc
      181 agaggtggcc cgcaggagaa aacgtcattt ctctgttctg ttcattgact gggaggccca
      241 gtatcagtgc accattgaac atattcagaa aatgcgggaa atgtaccggg atgtgacgga
      301 taccttttac tgggtggcac ttcccctgac cacggttaac ggtgtctcgc agtttcagcc
      361 ggagtggata tgctgggagt ctggtgtgac ctgggttcgc cagccaccgg aagaggccat
      421 tacagacatg acgtattttc ccttttaccg gtatgccatg gcgtttgaag aatttgttcc
      481 ggcattttct tcctggtttg ccggtaaccg gtgtggagtg gcaatactga ctggtgtccg
      541 tgctgatgaa tcgctcaatc gctttatggg actggtatct cagcgcaaac tgagatatgc
      601 agatgataaa ccctggacca cagcgtcgcc tgaagggttt tattacacca tgaatccgtt
      661 atatgactgg aaagcccgcg atatctggat atataacgcc agaacctgtg ccatctacaa
      721 tcctctgtat gacctgatgt accgtgccgg cgtgccgtta cgcaacatgc gtgtctgtga
      781 accttttggt cctgaacaac gtaagggact gtggctttac catgttctgg agccggaaac
      841 ctgggccagg atgtgtgagc gggtgtcagg cgctgccagc ggggcgcttt atgccaatca
      901 aagcggtgcc tattttgccc tgcgtaaacg tatatcaaaa ccggcccatc acgcctggcg
      961 cagctatgcg atgttcctgc tggatgtgat gccggaaaga acggcagaac attaccgtaa
     1021 taaaattgct gtctacctgc gctggtatca gacgcggggc ttcccggatg acatcccgga
     1081 tgaacaggag aatgacctgg ggagccggga tattccgtcc tggcggcgta tctgtaagac
     1141 acttataaag aatgatttct ggtgccggac cctctccttc agtccgaaca aaccccggca
     1201 ctatgaacgt tatctgcagc gtatgaaaga aaggaggaag gaatggggga ttctgtgaca
     1261 ccggagataa ttggcaacat gattcgtcag tacttcagcc aggaacgacc agaagaagag
     1321 accatacagg ccct
//
LOCUS       DAZFZC010000319         1331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFZC010000319 DAZFZC010000000
VERSION     DAZFZC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.329"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..470)
                     /locus_tag="ACHKXP_005413"
     CDS             complement(<1..470)
                     /locus_tag="ACHKXP_005413"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO7650170.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDR"
     gene            complement(534..>1331)
                     /locus_tag="ACHKXP_005414"
     CDS             complement(534..>1331)
                     /locus_tag="ACHKXP_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO7650171.1"
                     /translation="FLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFW
                     EQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETA
                     VVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQ
                     ILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVE
                     PKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI"
BASE COUNT          291 a          459 c          319 g          262 t
ORIGIN      
        1 cggtcgaaat atttaatggt ccggatggtg cattccgcat tcgcgggcac ggtgacggca
       61 gacacctcca tcagctccca gcgcagaaaa tgcagtccgc ctccgtccag ataagtgtat
      121 tcatggggcc ggaagcccac agaaagcccc ctgaccagcc cggtcttaat ggcagcccag
      181 gcctcatcca gccgggctgc catctgggag ggcatccccg gctccggctt caccagcatt
      241 gccgtgattt ccagcccttc cctgacccga cgcaccgtac actggcctac agggcgggaa
      301 tggtcatgct gccagagaaa cgggatcgta ctgccaaact ccgccccctc cggctccagg
      361 atgtcaccat cccgatccgg agaaggcgtt gacgcaatcc cggtgatcac ccgttcatcc
      421 tcgctgaagg atttcaccgt cagcagggaa caggcccgtt taagagtcac atcagcctcc
      481 tgaaaataaa aaaaccgccg cagcggttca tgatggttac agggtgagca gggttatatg
      541 aaaaaaacct catacgcttt ctttttcggt tccggattca gggacatcag ggacaccgca
      601 ttgaagagcg ccatcagcgg gtcaattttt ccccgtccgc tggcctgttt ggtgataaga
      661 atggcgttac ctttaggctc cacccgggca ttgccaacgc accaggccat cagtggctga
      721 ccaccatgca ccagtactcc ctcagccagt ttgcgctcgg tggtttttat ggccccgccc
      781 agcttccagc cctggcttat ccccacaaca attccgtcgg ggatcccggc ttccgccagt
      841 gaatccagaa tctgccccac acctgacggg tcaataccga tatgctccag taactcagcc
      901 tcatgaatac gacgcacata ctccgccact tccgccgtgt catccccgac ccgacggaca
      961 atcgtcatgt ctccacaggc cacaaaatcc tgaaaacggg atgcctcact cttccgtctg
     1021 accaccgcgg tttcatgcgc ccaggcatgg ccccagccca gccattcgcg ggtttccctg
     1081 tcacggccaa tcacgtacat tcccagcaga tcatccaggc ccccgccgtc aatccccacc
     1141 gtcaccacat cagcgcgctg caggatatcg tccaggctga cgcgcctgcc ctgctgctcc
     1201 cagaaatccg cacccgccca gcggtcagaa cgcagggcaa gaccaatttc cacattggca
     1261 tgttttgaca tgaagccacg aaatgcttcc tcaccagcct cccgggcttt acggtactcc
     1321 cggtacagaa a
//
LOCUS       DAZFZC010000320         1315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFZC010000320 DAZFZC010000000
VERSION     DAZFZC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.330"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(229..>1315)
                     /locus_tag="ACHKXP_005415"
     CDS             complement(229..>1315)
                     /locus_tag="ACHKXP_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFO7650172.1"
                     /translation="SRPEIMGWVLGIVYRVIATHLVKKAGHTHQVAKTGAVTLIQRFG
                     SALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQLTHTIAHRVGRYLERQG
                     LLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKVFTLQTLPTSGDPFGD
                     GIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLTRGGNVRYQLKTPYR
                     DGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALVTPAKRGRGNKVRV
                     ADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIEDPIVIKQILDHLK
                     HKAETSGTRALPESRAPPAELLLGLFD"
BASE COUNT          237 a          412 c          386 g          280 t
ORIGIN      
        1 ggaagaataa acgccctttt cacccaagtc caacagcttt ggaccgcagt tgactctttc
       61 gacacccctg cgatgcaacc caatccggct gacggggagc cagcaacgct gaaaatttac
      121 cctcctcttt cccactagcg gctccttttc cgacaaccag cacggcggat ccctgccgcg
      181 gcgctgtgaa cgcagcattt tgattggtat cgttggcctt caggctcgtc agtcaaacag
      241 acccaggagc agctcagccg gtggcgcccg gctttcgggt aacgccctgg tcccgctggt
      301 ttcggctttg tgcttcaggt gatcaaggat ctgcttgatc actatagggt cttcaatgca
      361 ggcgatgact ttcatggcgc cgccgcagcc gctgcaggtc tcgatgtcga tattgaaaac
      421 acgcttgagc cgttgcgccc atgtcatcga cgctcgccgt tgtgctggtg ttgccggttc
      481 atcagccacc ctgaccttgt tgcccctgcc ccgttttgcc ggcgtgacca acgcccggtg
      541 ccgactgttg ggtgcgaaca ccccgtggaa gcgggttagg ttgactctgg gcttcggtac
      601 cagggcggcc agccttgcaa tgaaatccaa tggttcgaaa atgacgtgcg tggtgccgtc
      661 ccggtacggc gtcttgagct ggtagcgcac gttgccgcct cgtgttaacg acagccgctt
      721 ctcggatacc gccgggcggc tgatgtaccg gcacagccgt tcgagcttct tgcgttcatc
      781 ggccctggcc gccacgccgg cgtgcaggct ggacccggct accttgccaa tcccgtcacc
      841 gaacggatca ccactggtcg gcagagtttg caaagtgaac acctttcgcc ccgcctgtga
      901 accgacagcg atacggtaag tgatcgagtg ccccagcagg ggtgtcatcg ggtcgtcatc
      961 caccgcatcc gaggccagat agctgttttc gacatcccgt tccagcaggc cttgccgttc
     1021 cagatagcga cccacccggt gggcgatggt gtgcgtcagc tgggtgagct ctgggctggt
     1081 cggcgccttg acccagcgga aacgcgctga gccgtgggat tgctcgacat acacaccgtc
     1141 gagaaacagc atgtggaagt gaacattcag attgagcgcc gatccaaaac gctggatcag
     1201 ggtgaccgcg cccgtcttgg ccacttggtg ggtatggccc gctttcttga ccaggtgcgt
     1261 ggcaatgacg cggtaaacga tgcccagcac ccaccccatg atctcgggcc ggctg
//
LOCUS       DAZFZC010000321         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFZC010000321 DAZFZC010000000
VERSION     DAZFZC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.331"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..1144
                     /locus_tag="ACHKXP_005416"
     CDS             32..1144
                     /locus_tag="ACHKXP_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HFO7650173.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT          323 a          314 c          352 g          302 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aaacgctgga agcagcgggc catgtgctat tgctaacatc
      901 attaccggaa gatgaatatt cagcagagca agtggctgat tgttaccgtc tgcgatggca
      961 aattgaactg gcttttaagc ggctcaaaag tttgctgcac ctggatgctt tgcgtgcaaa
     1021 ggaacctgaa ctcgcgaaag cgtggatatt tgctaatcta ctcgccgcat ttttaattga
     1081 cgacataatc cagccatcgc tggatttccc ccccagaagt gccggatccg aaaagaagaa
     1141 ctaactcgtt gtggagaata acaaaaatgg tcatctggag cttacaggtg gccattcgtg
     1201 ggacagtatc cctgacagcc tacaaaacgc aattgaagaa cgcgaggcat cgtcttaacg
     1261 aggcaccgag gcgtcgcatt cttcagatgg t
//
LOCUS       DAZFZC010000322         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFZC010000322 DAZFZC010000000
VERSION     DAZFZC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.332"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..256)
                     /locus_tag="ACHKXP_005417"
     CDS             complement(<1..256)
                     /locus_tag="ACHKXP_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650174.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTP"
     gene            complement(259..1026)
                     /locus_tag="ACHKXP_005418"
     CDS             complement(259..1026)
                     /locus_tag="ACHKXP_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650175.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMIEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYRGDYIRDNQDDIDNAITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWMIDECLCAMYDASSESPGK"
     gene            complement(1029..>1272)
                     /locus_tag="ACHKXP_005419"
     CDS             complement(1029..>1272)
                     /locus_tag="ACHKXP_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650176.1"
                     /translation="KDAWKFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNS
                     CIRPSFSLSRSAIIRLNVANARFVYQPFTRDQRARG"
BASE COUNT          371 a          292 c          281 g          328 t
ORIGIN      
        1 gaggcgttct gcgggagaat tgccaaccga tgccagccat tacactgaaa aaaagtgcat
       61 attcaaccat taactcaagt tgtagttcct gaagagaaat acgcacaacc aggagcggaa
      121 aggagaatat tatttctgcc agaaaagtta acgtgaatat aagtacggct aagacaataa
      181 ccgtcggcca taccaataat ggcacgataa caaaaccgag aattacaggg ggaatataaa
      241 gccggaattt aatcattttt attttcctgg tgattcggat gatgcgtcat acatcgcgca
      301 taaacactca tcaatcatcc agtcgtctac ccagacctga tgctgtgtgc cctgttctgg
      361 ctgctttggt gggttctcaa tcgcctgccg cgcgcgttct ttcaagatgg attgattgtt
      421 gaaggcccag gcattgattt gggtggtaat ggcgttatcg atatcgtcct ggttatctct
      481 gatataatct ccacgatatc tgttcaataa acaggcaaga gaaacggtcg accaaaagcc
      541 gggaactgaa ttaatggcat ccagcaatgg cgcaagggcg atatcggcac ggaaaaacat
      601 ttcaatcatg tactgtaatt cgacagcctc atcgccggat acccaatcag tcatatactg
      661 gcaggcaaat cgttgcaaaa ttttccattc atcctcacgc caattttctt tactggcaag
      721 gtttatacgc cgtagagaaa atacttcgtg aatattgtca aactgcccct gtgcaacaaa
      781 ttccagtaaa cgcggcagaa aatagcggac ctcatcgctg ttttgcggat cgggtcctgg
      841 gctactattc catgcattga ttaaggaata cgagatcgct cgtaacgatg tactccgtaa
      901 taccttttgt tcctgctgac tcaggcaata ttcacaacaa accacaaatt tctgcggcac
      961 cggataacga cgaaaaatat gataaatctg ctcaattaat gcctgcatgg cggggctggg
     1021 ttgcataatt accccctcgc ccgctgatcg cgtgtaaacg gttgataaac aaaacgagca
     1081 ttcgccacgt tcaggcgaat aatggcacta cgagagaggg aaaagctggg gcgaatgcaa
     1141 ctattgcagt ctcgacagct ttcttcagcg caattttcca ctgtgtaata acgagcgtag
     1201 agtttcagtt ccgcttgctt atcacgaaat ccgcgtaaac tgttatcgtt atgaaatttc
     1261 caggcatctt ta
//
LOCUS       DAZFZC010000323         1271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFZC010000323 DAZFZC010000000
VERSION     DAZFZC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.333"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            330..839
                     /locus_tag="ACHKXP_005420"
     CDS             330..839
                     /locus_tag="ACHKXP_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7650177.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            811..>1271
                     /locus_tag="ACHKXP_005421"
     CDS             811..>1271
                     /locus_tag="ACHKXP_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7650178.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGV"
BASE COUNT          338 a          261 c          328 g          344 t
ORIGIN      
        1 gcagatcaat taatacgata cctgcgtcat aattgattat ttgacgtggt ttgatggcgt
       61 agatgcacgt tgtgacatgt agatgataat tattatcatt ttgcgggtcc tttccggcga
      121 tccgacaggt tacggggcgg cgacctcgcg ggttttcgct atttatgaaa attttccggg
      181 atccatgtcc ggtttctctt caagttaact atatgaaaaa tataaaaaca ggtcttctgt
      241 gaaccggaca tgaacaaaaa acagacatgt aaaccggaca tgaccggttt tgttgtgatt
      301 gtgaggtgag agtttttgcg aggtgaggag tggctacgca gactgaagtt gccaggcatt
      361 taagtctgac cgatcgccag cttcgcagat tgcagaaatt gccgggtgcc ccgatatcga
      421 ataagcgagg gcaactggat ctggatgcct ggcgcgattt ttacatatcg tatctgagga
      481 gaagtaaaaa cgatgtgcct gatggcgata gcgaagacga ctatgaggag aaattgctta
      541 ttgccagatg ggaactgaca gcagaacagg ctgttacaca gcagttaaaa aatgaggtgt
      601 caaaaggaaa acttattgac accgggttct gtatttttgc cctcagtaag ctggcaatgg
      661 cgttatccag tacgcttgat tccatccctt tatccatgca gcgacagttt cctgatttaa
      721 caccgcgcca tcttgaccat ctgaaaaccc ttattgctaa gggggcaaat cagtgtgcgc
      781 gggcagggga taaattaccg gatttactcg atgaatatat cagagcaaca actgaataat
      841 atgatggctg ccgtttcggt tgcgctgcag cctctggtca gggttgtacc aatgacggca
      901 gttgaatggg ctgatcaaaa ttattatctg cctaaagaat cttcatatgg tgagggagaa
      961 tggaaaacgc tgccattcca gatcgccatt atgaactgta tgggtaacga ccaggttcgc
     1021 acggttaacc tgattaaatc tgcccgtgtt ggctatacaa agatgttgct gggggtggtc
     1081 gggtatttta ttgagcataa atcccgaaac agtctgcttt ttcagcccac ggattctgcc
     1141 gctgaagatt ttatgaagtc tcacgtggag gcgacgattc ggaacgtgcc atgcctgaaa
     1201 gacctttccc catggctggg tcgtaaacat cgtgacaata ctctcacgct gaaacgcttt
     1261 tcatcgggcg t
//
LOCUS       DAZFZC010000324         1261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFZC010000324 DAZFZC010000000
VERSION     DAZFZC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.334"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..289)
                     /gene="gnsB"
                     /locus_tag="ACHKXP_005422"
     CDS             complement(116..289)
                     /gene="gnsB"
                     /locus_tag="ACHKXP_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO7650179.1"
                     /translation="MNIENLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(461..691)
                     /gene="ynfN"
                     /locus_tag="ACHKXP_005423"
     CDS             complement(461..691)
                     /gene="ynfN"
                     /locus_tag="ACHKXP_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO7650180.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(696..761)
                     /gene="ynfR"
                     /locus_tag="ACHKXP_005424"
     CDS             complement(696..761)
                     /gene="ynfR"
                     /locus_tag="ACHKXP_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFO7650181.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(764..952)
                     /gene="ynfQ"
                     /locus_tag="ACHKXP_005425"
     CDS             complement(764..952)
                     /gene="ynfQ"
                     /locus_tag="ACHKXP_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO7650182.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(963..1175)
                     /gene="cspI"
                     /locus_tag="ACHKXP_005426"
     CDS             complement(963..1175)
                     /gene="cspI"
                     /locus_tag="ACHKXP_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO7650183.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          411 a          243 c          206 g          401 t
ORIGIN      
        1 ctgcattggt ttaataaaaa ccatcaacaa ttgtgattta gatattcgga accattcaaa
       61 tataacaaaa ccccgtaaaa acgaggttta tggataaatt ttattattga atacatcaga
      121 ttaaattaat cttgacatca tagctttcaa gacccgtcat tttttcccgt gcggtaaact
      181 gaatactggt aacttctttc ccggtctttt tcttaagttc aataattttt tttgttatat
      241 attcagaaat atctgcttct gcttttgttt ttaagttttc aatattcatc atttcctctt
      301 ttagtctgtt atgactttcc agttacacag taagtcgatt atatggtgca aacgtgtaaa
      361 agataagatg aaacatcgca ataatcaaca tacgatagtc taaattttac acaaacagac
      421 aaagagaatt ttcctgaatt atcaatgcaa tagcatcaaa tcaactcaag agccttattg
      481 ctgcttccag aatttcttct gaagtaacat gtcgatccgc ggctacataa atgactttat
      541 gatctccggt cagagatgga aaccctgcgg ccattacagt aaggtgtgtt ttttcgccat
      601 ttggatattc acgcatgatg gtgttaactc cagtcatcgc tggcactacc actgctggtt
      661 cagagttaaa aaaaactatg atttttttca tgatgttacc gtagtatgtg agtatccatc
      721 gaatagacac caagcaaaaa agctcccgaa ggagccttca ttttcacttt tttaaatcca
      781 acgacagacg gctggcattt aagtattgtg aaatattatc aaatgtaatc atcattgatt
      841 tacaaaagat acattttgcc ccgaaaggat tcatgtcaga aacatcaaaa gatgatgttc
      901 tatactggga accatgacaa cacgggcatc taaagtgaat atggtttgta atattgtcta
      961 cctcaaagcg ccactacatg aacagcggca ggacctttag gtccgttctc aataccaaat
     1021 tcaacttcct gattctcagt taatgttttg aaatcgttgc tctgaattgc tgagaaatgg
     1081 acaaacacat ctttgctgcc atctttcggc gtgatgaaac caaaaccttt ttcagggtta
     1141 aaccatttca ctaaaccagt cattttgtta gacataatta ttaccttttg aagaaattag
     1201 cccttgggca gaatggtccg aaaaaaaata tcagagagaa aaaccaacaa ggaaatctca
     1261 a
//
LOCUS       DAZFZC010000325         1245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFZC010000325 DAZFZC010000000
VERSION     DAZFZC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.335"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(438..656)
                     /locus_tag="ACHKXP_005427"
     CDS             complement(438..656)
                     /locus_tag="ACHKXP_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650184.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINDRFSRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCALGVNS"
BASE COUNT          434 a          236 c          214 g          361 t
ORIGIN      
        1 ggtattctgg gaactatatt ctcaaaatac agtagaaacg aggtatgttt ctggtggaaa
       61 ggacagtggg attaaaaagt aagaattgat aaaaaaaacg ccagcaacac atagtgccgg
      121 cggagggaat accccatgga gaaaatgtga tgcctagagc atcatgaata tataattaaa
      181 aatagttagc gttgtcaata ctttcacaaa aataattttc gtagtataga aagatatttt
      241 tatgcatgac ctacctgaat ttgctccggg tagaggttat aaataaaaat tgaatcacga
      301 caaacacaat attcatagta tggcgatgcc tacgccagca agaatagcgc caataatatt
      361 gggaatataa tagacaccag acgcacaggc atctcactcc ttaacaaaca acaatcagga
      421 tatttacttt taccaagcta actgtttaca cccaaagcgc acacaattaa ccattcaata
      481 acaaatggca atatccatag ccatacgact ttaccttgta atgttcggta tttctttata
      541 attattctgg aaaatctatc atttattttt aaaatcaaat tatcttgttg tttaaaaata
      601 agttcacata ctttatcatc ttcttccgcc atcaacatct ctgcatactt aaacatttca
      661 gaacgttcct ttagcacaga agagtaatta tatgtccagt tcccaggcaa taatgcttat
      721 ggatatttaa ttcataattt agagaatatt ttgcaataat atttggcagt attcagaaat
      781 acctgaaaaa tcatactata cagccccagg gaatggataa agattctaac aaagcatttc
      841 aacaatatat acttgttaaa aatccatatc gaaatgccgt tgcagcatta atatatgcct
      901 cattcataaa atgtaaaaga gcaagctcgt accagtaggg gggaaattca ttgacatgtc
      961 ctgtcaatac gtacagagcc ctgccatgct tgccagcacg taacaattcc gcccccagta
     1021 accagcgtgt cccctgatta ttctcagggt tatatgttag tattttatca atgagtataa
     1081 cggcatcctg atgacgctgt aaatgaacat tcgccagaat tgcagcataa agagcgcaaa
     1141 tgcatatgcc agatgagcgt gaatatcaat gatgtcagga gcatgtcagg caagacgcct
     1201 gagtttgttg acgtaatttg tttcgtttat ttcatcacta tcgta
//
LOCUS       DAZFZC010000326         1239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFZC010000326 DAZFZC010000000
VERSION     DAZFZC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.336"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            143..1239
                     /locus_tag="ACHKXP_005428"
                     /pseudo
     CDS             143..1239
                     /locus_tag="ACHKXP_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT          298 a          354 c          379 g          208 t
ORIGIN      
        1 acggacggag aaatgctgtc agcaaaaaga cttccgcttc gcgtatctgt ggggcatggg
       61 gataatttat cttcatcaat tgatatattg attaatggca aggaggcagt tgaaattccg
      121 taaggccaac ttgatatgac acctggcaca caaccccgac gaccggatgt acatgctggg
      181 cgggatgagt gacaccgcca gcaaccgcat cacgtttgag cgcgacagcc agtaccgaat
      241 cacgggtgtc agtcacaccg acgggatccg gcttaaactg acgtaccatg ccagcggcta
      301 cctgaaagcc attcaccgca cggataacgg catacaaacg ctggcgacct acgaacagga
      361 tgcgcggggg cggctgaccg aagcggatgc gcagctggac taccacctgt tttatgagta
      421 cgacgctgcg gaccggatca tccgctggtc cgataacgac cagacgtgga gccgtttcac
      481 ctacgatgaa cagggccggt gcgtgaatgt caccggggcg gacggctatt acaacgccac
      541 gctggactat agtgacggct gcaccaccgt gacggacggc aagggcactc accgttatta
      601 ctatgatcct gacggcaata ttctgcggga agaagcgccg gacggcagca ccaccacgta
      661 tgaatgggat gaattccatc acctgctggc ccgccactcc cctgccgggc gggtggagaa
      721 gtttgaatac aacgccgcac acggtcagtt aagccgttat acggcggcag acagcgcggt
      781 gtggcagtac cgctatgatg agcgcggcct gctcagcaac atcaccgacc ctgccgggca
      841 gacgtggacg cagcagtgcg atgaacgcgg cctgccggta agcctggtgt cgccacaggg
      901 tgaagagacc cgcctggcgt acacgccgca ggggctgttg tcgggaatat tccgccagga
      961 tgaacggcgc ctgggcataa agtacgacca ccacaaccgg ccggaaacac tcaccgacgt
     1021 gatgggccgt gaacaccaca ccgaatacag cggtcacgac ctgccagtga agatgcgcgg
     1081 ccccggcggt cagtcagtgc ggttacagtg gcagcagcac cataaactga gcggcattga
     1141 acgtgctgga accggcgcgg aaggatttcg ctatgaccgc cacggcaacc tgctggcctg
     1201 gacggacggc aacggtgttg tctggacgat ggaatacgg
//
LOCUS       DAZFZC010000327         1223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFZC010000327 DAZFZC010000000
VERSION     DAZFZC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.337"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..199)
                     /locus_tag="ACHKXP_005429"
     CDS             complement(<1..199)
                     /locus_tag="ACHKXP_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7650185.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYLCNEICGTKIALERRSKEREK
                     AEKAAEKKRRREEQKQKDKLKI"
     gene            complement(174..740)
                     /locus_tag="ACHKXP_005430"
     CDS             complement(174..740)
                     /locus_tag="ACHKXP_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7650186.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          371 a          265 c          192 g          395 t
ORIGIN      
        1 gaatcttaag tttatctttc tgtttctgct cctctcgtcg tcgtttcttc tctgctgctt
       61 tttccgcttt ttcgcgttct ttacttcgtc gttcgagtgc tatcttggtt ccacaaatct
      121 cattacacaa atattgattt tgatattttg gtataaacca ttcattgcaa catttacatt
      181 tccttcgata gattcgcata agtgctcctt tcgttgccgg aaaaatcacc gtaatactta
      241 tctcgggctt cttcagcaac tagtaccgct aactccagat catcaaagca tccgaagtgt
      301 ttactctttc catggaatcc tagcctaaca ttccattttt tctgtcgttt gtgccaagta
      361 acccctctgc aacctgattt gctattcttt cggatcctta tatttcttga attttctatt
      421 ggcaggcatt ctcttaaatt ttctggccta ttgtcggtcc taattccatt aacgtggtca
      481 atttgaccag caggccaacg attatgagtt atgtaaaaaa ctaagacgtg agttttatat
      541 ctacgcccat ctatcatgat cattgaataa ccgttggaat caaaagttcc agcaacacta
      601 tttaatgcta tccttccctg agtgggaact ttccatctaa ataccccggt agatttatcg
      661 aaacttagta actcaaatat ccttttaaca gttaaatctt ctcttttacg gttacatcgt
      721 cttcgcgctg gtttagccat cgccttcttc ctccgtaatg gttttctgaa tttggccacc
      781 tgaacagagc tcaccaaagc tatggatgtc ggtatttcca caataccaag atggcgaaaa
      841 taactgcatg ataagcctca gggaaaaggg aagacactac ccccgataat tcagaaacaa
      901 atcgaaatac atgaactaaa gaaaaaatca caacaaatag aaacagaaat agaaatagaa
      961 atagaaatag aaatagaaat agaaatagaa atagaaatat taaaacagac caccacattc
     1021 ctgatgtcat actcactaaa caatttttcg acaacatggt agctcatagc atgttatcgt
     1081 gtagacaccc tctgctacgt attcggtgtt cagtgcaata tctacaaata ctggaaaaat
     1141 ctaaaatcga ggcaagtttt tagtgataac tatagttaga ctatattgac gacctgatgt
     1201 gctgtatgta ataattaaca aaa
//
LOCUS       DAZFZC010000328         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFZC010000328 DAZFZC010000000
VERSION     DAZFZC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.338"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..704
                     /locus_tag="ACHKXP_005431"
     CDS             3..704
                     /locus_tag="ACHKXP_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7650187.1"
                     /translation="MKNIAAQMVNFDCEQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            701..991
                     /locus_tag="ACHKXP_005432"
     CDS             701..991
                     /locus_tag="ACHKXP_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7650188.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            1065..>1222
                     /locus_tag="ACHKXP_005433"
     CDS             1065..>1222
                     /locus_tag="ACHKXP_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7650189.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWAC"
BASE COUNT          344 a          294 c          332 g          252 t
ORIGIN      
        1 ctatgaaaaa catcgccgca cagatggtta actttgactg tgagcagatg cgccggatcg
       61 ccaacaacat gccggaacag tacgacgaaa agccgcaggt acagcaggta gcgcagatca
      121 tcaacggtgt gttcagccag ttactggcaa ctttcccggc gagtctggct aaccgtgacc
      181 agaacgaact gaacgaaatc cgccgccagt gggttctggc tttccgggaa aacgggatca
      241 ccacaatgga acaggttaac gcaggaatgc gcgtagcccg tcggcagaat cgaccatttc
      301 tgccatcacc cgggcagttt gttgcatggt gccgggaaga agcatccgtt atcgccggac
      361 tgccaaacgt cagcgagctg gttgatatgg tttacgagta ttgccggaag cgtggtctgt
      421 atccagatgc agagtcttat ccgtggaaat caaacgcgca ctactggctg gttaccaacc
      481 tgtatcagaa catgcgggcc aatgcgctta ctgatgcgga attacgccgt aaggccgcag
      541 atgagcttgt ccatatgact gcgagaatta accgtggtga ggcgatccct gaaccagtaa
      601 aacaacttcc tgtcatgggc ggtagacctc taaatcgtgc acaggctctg gcgaagatcg
      661 cagaaatcaa agctaagttc ggactgaaag gagcaagtgt atgacgggca aagaggcaat
      721 tattcattac ctggggacgc ataatagctt ctgtgcgccg gacgttgccg cgctaacagg
      781 cgcaacagta accagcataa atcaggccgc ggctaaaatg gcacgggcag gtcttctggt
      841 tatcgaaggt aaggtctggc gaacggtgta ttaccggttt gctaccaagg aagaacggga
      901 aggaaagatg agcacgaacc tgatttttaa ggagtgtcgc cagagtgccg cgatgaaacg
      961 ggtattggcg gtatatggag ttaaaagatg accatctaca tcactgagct tgtaacaggt
     1021 ctgctggtaa tcgcaggcct ttttatttgg gggagaggga agtcatgaaa aaactaacct
     1081 ttgaaattcg atctccagca catcagcaaa acgctattca cgcagtacag caaatccttc
     1141 cagacccaac caaaccaatc gtagtaacca ttcaggaacg caaccgcagc ttagaccaaa
     1201 acaggaagct atgggcctgc tt
//
LOCUS       DAZFZC010000329         1204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFZC010000329 DAZFZC010000000
VERSION     DAZFZC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.339"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..628)
                     /locus_tag="ACHKXP_005434"
     CDS             complement(<1..628)
                     /locus_tag="ACHKXP_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7650190.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMSADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVL"
     gene            complement(871..1077)
                     /locus_tag="ACHKXP_005435"
     CDS             complement(871..1077)
                     /locus_tag="ACHKXP_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO7650191.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          277 a          341 c          304 g          282 t
ORIGIN      
        1 tcagtacccg gtacggcggc agcggcgcag gcagcacctc atccgcttcc ggcacccgct
       61 cacaccagcc gagcagccac cacggcccct gcggactgtt tgtcgccaga taacgatgcg
      121 gacttaagcg gagttcttcc ggcagcgcct gccagagtgc ggccagccgg tgaccttcat
      181 ccagtttcgc cacgccgccg cgcaccagcc acagtgactc gctgcggctg taaccgtcct
      241 caccgggaaa caggtgctca aaatacaggc ttctgccgcc gttatcactg agtatcagtg
      301 tgttatcgcg cagctgtaag cggatatccg cagacatttt ccagccgggg ccgaggctcc
      361 ccaccggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagaga atgaacggca
      421 gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcagcggat
      481 tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg
      541 caccgatgcg taccccggct gaaccctgga caatgctacc gccatactgc gtcatgtcgc
      601 cctgacgcgc cgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc
      661 tgctgacagg cgaaacccta cagatttata agaaagcctt aagtgagata tctgtcagat
      721 aagaacaatt aggaaaatat ttatatatat ttcatgaata tatgtttcga ttggtataac
      781 gatgtctaaa tattcctctg aacatcttcc tgatacaaaa aaccgccagc gtttccgctg
      841 gcggttttca atgtattcaa aactcagcaa tcaataaaaa tcaccagttg ccttttcggc
      901 ctgatccatc cacaccggtt tatcgctggt tttcgaccag acgcgatgca ggtagctgta
      961 aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc gccagaacgc ccgctgcaac
     1021 cgcataagta cggcgtaaaa agataagcca tgcaggatat tctctgtaga gttccatact
     1081 tttctccccc ttttgtatct acccggtgaa tggcaccgga aaaatgaatt tgtttatctg
     1141 atgaaaatag taccgccttt tgtgtaattt tactactcat ccgaccactt atttttgctt
     1201 attg
//
LOCUS       DAZFZC010000330         1191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFZC010000330 DAZFZC010000000
VERSION     DAZFZC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.340"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..681
                     /locus_tag="ACHKXP_005436"
                     /pseudo
     CDS             49..681
                     /locus_tag="ACHKXP_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(700..1191)
                     /locus_tag="ACHKXP_005437"
                     /pseudo
     CDS             complement(700..1191)
                     /locus_tag="ACHKXP_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
BASE COUNT          384 a          183 c          225 g          399 t
ORIGIN      
        1 gatgattata aatgaatacg ttaaagcatt tacactcagg atgaatatat gggaaaaatt
       61 aaaattgtag tttcagatca gcagccgttt atgattgatg ggataattgg atttctcgga
      121 cattatcctg atttatatga ggttgttggg ggctataaag atctgaagaa agctatagcg
      181 gagtgtaaca aatctacagc ctaaatattt attttaggag aatttgcagg tggaatgatg
      241 gggtccgaac tcgtaaaatg ggttaaatcg cataagatag atgctcatat tattacattt
      301 gtagcaaaga tgccgtatat tgattcaata aaattgcttg aagcaggtgc gaaaggatgc
      361 gtatggaaaa ccagtcaccc ggcgaaacta aatcgtgcta ttgattcgat tagtaatggt
      421 tacacttatt ttgatagtgt acatatggat tgtgaaaaaa tatcttccag gtattcctct
      481 gataatcaac ttacaaatcg tgagtcagaa atattacaac tcatagctga tggtaaaaca
      541 aacaaagaaa tcgctaattt cttgcagtta agcaggaaaa cagtcgaaac tcatagactc
      601 aatatcatga agaaattaga tgttcacagt ggtatcgagt tgatcaaaac agctttacgt
      661 atgggtgttt gcactatata aaaaaaagag gctttaggct cattttgctg aagccttacg
      721 attgtctagt tcattaagcc tggtatatac aatatatatt ttggggcata ttgctgtctc
      781 tttttttggt tatccattgc aaaacccgca ttataagtcc ccaggcattg ccacgttact
      841 ccacaacgtg aaaaatggtt atataaaatc tcggttccta ttttaatgtt cagacaaggg
      901 ttatctaaca aatctcgttc atcttttatt attccacgtt tttttaacag gggaatatgc
      961 atctggttta tctgcattaa ccaataatct aaactcttta tattgttatt cttatctctg
     1021 ttcttgccta tgctatcctt ttttagattg ctttcaacaa gagcgatagc tttaattaaa
     1081 tttggttcga ttctgaacat tgttcctgct tcattagagc aagagttgct ggcgaaagac
     1141 atttttgtaa taaatataca aaacgtgaag atggttaaga atttaacttt t
//
LOCUS       DAZFZC010000331         1191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFZC010000331 DAZFZC010000000
VERSION     DAZFZC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.341"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..803
                     /gene="corA"
                     /locus_tag="ACHKXP_005438"
     CDS             <1..803
                     /gene="corA"
                     /locus_tag="ACHKXP_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO7650192.1"
                     /translation="ATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAF
                     TIRDGRLFTLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIY
                     SDLEQLSRVIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPG
                     GQLEQAREILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLP
                     PTLVASSYGMNFEFMPELKWSFGYPAAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(846..>1191)
                     /locus_tag="ACHKXP_005439"
     CDS             complement(846..>1191)
                     /locus_tag="ACHKXP_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFO7650193.1"
                     /translation="EKWKYRFNFYDQHGFPGFWGATPEYKAAFKALKVRQRLTIQMNF
                     IAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVAAYVAVNTNKWFY
                     EKEVKGLNTWSL"
BASE COUNT          307 a          282 c          285 g          317 t
ORIGIN      
        1 tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt gaagacgacg
       61 acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac gccggtaact
      121 ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag cgtgaactgc
      181 ccgcttttcg tctgtatcgt atgcgtgccc gtagccagtc gatggtagac ggtaacgcct
      241 acgagttgct gctggatctg ttcgaaacca aaatcgaaca gttggcagat gaaattgaaa
      301 atatctatag cgacctggag cagttgagcc gggtgattat ggaagggcat cagggcgatg
      361 agtacgacga ggcgctctcc actctggcgg aactggaaga tatcggctgg aaagttcgcc
      421 tgtgtctgat ggatacccag cgcgcgctca acttcctggt gcgtaaagcg cgtttaccgg
      481 gtgggcaact ggagcaggcg cgtgaaatcc tgcgagatat cgaatccctg ctgccgcata
      541 acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcaatgggt tttatcaaca
      601 tcgagcagaa ccgcatcatc aaaatcttct cggtggtatc cgtggtattc ctgccgccga
      661 cgctcgttgc ttccagctat ggcatgaact ttgagtttat gccagaactg aagtggagct
      721 tcggctaccc tgccgcgatt atctttatga tcctcgcggg cctggcaccg tatctgtact
      781 ttaagcggaa gaactggttg taaaaaacga gagcggtggc ttagtctggc taagccacct
      841 gttattcaaa ggctccaggt atttaaccct tttacctctt tctcatagaa ccatttgttc
      901 gtgttaacag caacataggc tgctacggca attcccagaa tgttaacgcc aattagtgca
      961 ccaacaaata gagatagaat accaagtaat agaactataa ttgctttttt ccataacccc
     1021 aggacaaata aatatatcca ggaacagaag aaagcaatga aattcatttg aatagttaag
     1081 cgttgtctta cttttaatgc tttaaatgct gctttatatt ctggtgttgc cccccagaat
     1141 ccaggaaaac catgttgatc ataaaaatta aatcggtatt tccatttttc a
//
LOCUS       DAZFZC010000332         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFZC010000332 DAZFZC010000000
VERSION     DAZFZC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.342"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            51..>1177
                     /gene="rtcB"
                     /locus_tag="ACHKXP_005440"
     CDS             51..>1177
                     /gene="rtcB"
                     /locus_tag="ACHKXP_005440"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO7650194.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDE"
BASE COUNT          329 a          261 c          334 g          253 t
ORIGIN      
        1 acgacggttg caattatcag gacagcaaac aacgaaaaga gaaaaacaaa atgaattacg
       61 aattactgac cactgaaaat gccccggtaa aaatgtggac caaaggcgtg ccggtagagg
      121 ccgatgcgcg tcagcaactt attaatacgg cgaagatgcc gtttattttc aaacatattg
      181 cggtaatgcc tgatgtacac ctgggtaaag gttccaccat tggtagcgtg atcccgacca
      241 aaggggcgat tattccggcg gcggtgggcg tggatattgg ctgtggaatg aacgcgctgc
      301 gtaccgcgtt aacggcggaa gacctgcctg aaaacctggc agagctgcgt caggcgattg
      361 aaacggccgt gccgcacggg cgtaccactg gccgttgtaa acgtgataaa ggtgcctggg
      421 aaaatccacc tgttaacgtc gatgctaaat gggctgagct tgaagccggt tatcagtggt
      481 taacgcaaaa atatccccgt ttcctgaata ccaataacta taaacacctg ggaacgctgg
      541 gaaccggtaa ccactttatt gaaatctgcc ttgatgagtc ggaccaggtg tggattatgc
      601 tgcactccgg ttcacgcgga attggtaacg ccatcgggac ttactttatc gatctggcac
      661 aaaaagagat gcaggaaacg cttgagacgt tgccgtcgcg tgatctggcg tactttatgg
      721 aaggtacgga atactttgat gattacctga aagccgtggc ctgggcgcag ctttttgcca
      781 gccttaaccg cgatgcgatg atggaaaacg tggtaacggc attgcagagc attacgcaga
      841 aaacggtcag acagccacaa acgctggcga tggaagagat caactgtcac cacaactatg
      901 tgcaaaaaga acagcacttt ggtgaagaga tctacgtgac gcgtaaaggc gcggtgtctg
      961 cgcgtgctgg tcaatatgga attattcccg gttcgatggg agcaaaaagc tttatcgtcc
     1021 gtgggctggg aaatgaagag tcgttctgtt cgtgcagcca cggtgccggg cgggtaatga
     1081 gccgaactaa agcgaaaaaa ctgttcagcg tggaagatca aattcgtgcc accgcgcatg
     1141 tggaatgccg taaagatgcc gaagtgatcg acgaaat
//
LOCUS       DAZFZC010000333         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFZC010000333 DAZFZC010000000
VERSION     DAZFZC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.343"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1177
                     /locus_tag="ACHKXP_005441"
     CDS             <1..>1177
                     /locus_tag="ACHKXP_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO7650195.1"
                     /translation="EREREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLKALQK
                     ESDTLTGRVDDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAASCCPECGGSLSYLGED
                     AAEQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLISKY
                     AEHTPLYRQSEMYGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDT
                     PVPVLLPGNKKTKTGRLWTYVRDDRNAGSTLAPAVLFAYSPDRKGIHPQTHLAGFSGV
                     LQADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEA
                     EIRGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTY
                     "
BASE COUNT          260 a          349 c          364 g          204 t
ORIGIN      
        1 cgagcgtgaa cgcgaaatag accatctgca ggcacagctg gataagctgc gccggatgaa
       61 cttcggcagc cgctccgaaa aagtctcccg tcgtatcgca cagatggaag ctgacctgaa
      121 ggcacttcag aaagaaagtg atacccttac cggtcgggtt gacgacccgg ccgtgcagcg
      181 cccgctgcgt caaacccgca cccgcaaacc gttccccgaa tcactccccc gcgatgaaaa
      241 acggctgctg ccggcagcat catgctgccc ggaatgtgga ggctcactga gctatctggg
      301 tgaggatgcc gccgaacagc tggagttgat gcgcagcgcc ttccgggtta tccggactgt
      361 acgtgaaaag catgcctgta ctcagtgcga tgccatcgtg caggcccccg cgccttcacg
      421 gcccatcgag cggggtatcg caggaccggg gctgctggcc cgcgtgctga tctcaaagta
      481 tgcagagcac accccgctgt accgccagtc tgaaatgtac ggccgccagg gcgtggagct
      541 gagtcgttca ctgctgtcgg gctgggtgga tgcatgctgc cggctactgt caccgctgga
      601 agaagcgctt caggactatg tgctgactga cggtaagctc catgctgatg acacgcctgt
      661 cccggtgctg ttgccaggca ataagaaaac gaagaccggg cggttatgga cctacgttcg
      721 tgacgaccgt aacgccgggt caacgctggc gccggcggtg ttgttcgctt acagcccgga
      781 cagaaaaggc atccatccgc agacccatct tgcggggttc agtggtgtac tgcaggcgga
      841 tgcatacgcc gggttcaacg agctgtaccg ggatggccgg ataacggaag ccgcctgttg
      901 ggctcacgcc cgccgtaaaa tccacgatgt gcacgttcgc accccgtcag ccctgacgga
      961 ggaagcgctg aaacggatcg gcgaactgta cgccatcgag gcagagataa ggggaatgac
     1021 ggcggagcag cgccttgccg aacgtcagtt gaaaacgaaa ccgctgctga aatccctgga
     1081 aagctggctg cgtgaaaaga tgaaaaccct gtcgcgacac tcagaactgg cgaaagcgtt
     1141 cgcatacgcc ctgaaccagt ggccggcgct gacgtac
//
LOCUS       DAZFZC010000334         1158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFZC010000334 DAZFZC010000000
VERSION     DAZFZC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.344"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..955
                     /locus_tag="ACHKXP_005442"
     CDS             <1..955
                     /locus_tag="ACHKXP_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7650196.1"
                     /translation="EYKRQLAALGDKVTYQERLNALAQQADKFAQQQRAKRAAIDAKS
                     RGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWS
                     EWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQA
                     MVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRG
                     EFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINND
                     GTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            952..>1158
                     /locus_tag="ACHKXP_005443"
     CDS             952..>1158
                     /locus_tag="ACHKXP_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7650197.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWK"
BASE COUNT          267 a          281 c          398 g          212 t
ORIGIN      
        1 ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtt acgtatcagg agcgcctgaa
       61 cgcgctggcg cagcaggcgg ataaattcgc acagcagcaa cgggcaaaac gggccgccat
      121 tgatgcgaaa agccgggggc tgactgaccg gcaggcagaa cgggaagcca cggaacagcg
      181 cctgaaggaa cagtatggcg ataatccgct ggcgctgaat aacgtcatgt cagagcagaa
      241 aaagacctgg gcggctgaag accagcttcg cgggagctgg atggcaggcc tgaagtccgg
      301 ctggagtgag tgggaagaga gcgccacgga cagtatgtcg caggtaaaaa gtgcagccac
      361 gcagaccttt gatggtattg cacagaatat ggcagcgatg ctgaccggca gtgagcagaa
      421 ctggcgcagc ttcacccgtt ccgtgctgtc catgatgaca gaaattctgc ttaagcaggc
      481 aatggtgggg attgtcggga gtatcggcag cgccattggc ggggctgttg gtggcggcgc
      541 atccgcgtca ggcggtacag ccattcaggc cgctgcggcg aaattccatt ttgcaaccgg
      601 aggatttacg ggaaccggcg gcaaatatga gccagcgggg attgttcacc gtggtgagtt
      661 tgtcttcacg aaggaggcaa ccagccggat tggcgtgggg aatctttacc ggctgatgcg
      721 cggctatgcc accggcggtt atgtcggtac accgggcagc atggcagaca gccggtcgca
      781 ggcgtccggg acgtttgagc agaataacca tgtggtgatt aacaacgacg gcacgaacgg
      841 gcagataggt ccggctgctc tgaaggcggt gtatgacatg gcccgcaagg gtgcccgtga
      901 tgaaattcag acacagatgc gtgatggtgg cctgttctcc ggaggtggac gatgaagacc
      961 ttccgctgga aagtgaaacc cggtatggat gtggcttcgg tcccttctgt aagaaaggtg
     1021 cgctttggtg atggctattc tcagcgagcg cctgccgggc tgaatgccaa cctgaaaacg
     1081 tacagcgtga cgctttctgt cccccgtgag gaggccacgg tactggagtc gtttctggaa
     1141 gagcacgggg gctggaaa
//
LOCUS       DAZFZC010000335         1114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFZC010000335 DAZFZC010000000
VERSION     DAZFZC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.346"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            21..479
                     /gene="etgA"
                     /locus_tag="ACHKXP_005444"
     CDS             21..479
                     /gene="etgA"
                     /locus_tag="ACHKXP_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO7650198.1"
                     /translation="MKKIILSIILISNSCYASDCFEITGKAYNIDPLILKAIAWNESK
                     NKNGIKSKMNKNGTYDIGIMQINSSHLDLLSKFNISEDDLLNDACINISVAGYILASN
                     IKSRGNTWDAVGAYNAGYFNTPNAVELRRQYAMKIYKTYTKLKNNEQIID"
     gene            complement(476..>1114)
                     /gene="escU"
                     /locus_tag="ACHKXP_005445"
     CDS             complement(476..>1114)
                     /gene="escU"
                     /locus_tag="ACHKXP_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO7650199.1"
                     /translation="KSIFELLKSVFKLVIITLIFYFMGHSYANEFANFTRLNAYQTLV
                     VVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKG
                     ERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQ
                     IIKLAELYDIPVIEDIPLARSLYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
BASE COUNT          405 a          187 c          176 g          346 t
ORIGIN      
        1 tattgaatat caacatgaaa atgaaaaaaa ttatattgag tatcattctc atttcaaatt
       61 cttgttatgc ttctgattgt tttgaaatta ctggaaaagc atataatata gacccattaa
      121 tattaaaagc aattgcatgg aacgaatcaa aaaacaaaaa cggaataaaa agtaaaatga
      181 acaaaaacgg aacgtatgat ataggtataa tgcaaataaa ttcatctcat ttagatttat
      241 taagcaaatt taatatatct gaagatgatt tattgaatga tgcctgtata aacatatctg
      301 ttgctggtta tatcttagcc tctaacataa aatccagggg gaatacatgg gatgcagttg
      361 gtgcgtataa tgcaggctac tttaacacac caaatgcagt agaactcaga agacaatatg
      421 caatgaaaat atataaaaca tacactaaat taaaaaacaa tgaacaaatt atcgattaat
      481 aatcaagatc tattgcaata cgaatcaatt gtgctacagg ttcaaaaaag tcttctgtta
      541 tgtattgtcc tttatgtatg ttcttataaa gacttcttgc caaaggaata tcttcaatca
      601 ctggaatatc atagagttca gccagtttaa tgatctgtag agctttggca tcttttcctg
      661 tttcaataac taaaggcaat ggagtctccc cgagtttata gtataggcaa atcgcaatgt
      721 gagtcgggtt tttgacgatc acggttgatt ttttgatgtt attagccaga cttccacttt
      781 gtatctcaga atgaaggcga cggcgctccc ctttaatttc agggttacca tcagtatcct
      841 tggcttctcg tttcacctca tctttactca ttttcatttt cttcagtccc tcatgcttct
      901 ggaaccagaa atcaaatact gaaaagagta gatatccgaa taacacgcct ttccataaaa
      961 gaaaaacaaa aaaggcaaca acgacaagag tttgatatgc gttcagtctt gtgaaattag
     1021 caaactcatt tgcatatgaa tgccccataa agtaaaaaat gagagtaatt atcactaact
     1081 taaatactga cttaagcagc tcaaaaatac tctt
//
LOCUS       DAZFZC010000336         1103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFZC010000336 DAZFZC010000000
VERSION     DAZFZC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.347"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            103..378
                     /gene="yddL"
                     /locus_tag="ACHKXP_005446"
                     /pseudo
     CDS             103..378
                     /gene="yddL"
                     /locus_tag="ACHKXP_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            417..>1103
                     /locus_tag="ACHKXP_005447"
     CDS             417..>1103
                     /locus_tag="ACHKXP_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7650200.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVTGNKNELL
                     LLDLSHNKLTS"
BASE COUNT          397 a          209 c          179 g          318 t
ORIGIN      
        1 taatcaccat tctattttaa aaacagtatg tcacttataa atgacgccgt ttaaaattcg
       61 tttagaaaat acgtttaacg ataattaaaa ggattctgtt aaatgaaatt aaaaatagtt
      121 gcggtggttg taactggttt gttagctgcg aacgtagcac acgctgccga agtctataac
      181 aaggatggta ataaactcga cctttatggc aaggttaccg ctctacgtta ttttactgat
      241 gataagcgtg acgatggtga taaaacttat gcccgtctcg gctttaaagg agaaacgcaa
      301 atcaatgatc aaatgattgg ttttggtcac tgggaatatg attttaaagg ctataacgat
      361 gaagccaacg gctcgcgcgg caagaatctc tgattgagtt aagtgcccat cacaatatga
      421 tcactgacct tatattacac aatcatccca ggatgaaaac aatcacttta aacgacaacc
      481 atattgcaca tttaaacgcc aaaaacacta caaaactgga atatttaaac ttaagcaata
      541 acaatttact gccaaccaat gacattgatc aactaatatc atcaaaacat ctttggcatg
      601 tattagttaa cggcatcaac aatgatccac ttgcacaaat gcagtactgg actgcagtaa
      661 gaaatataat tgatgacact aatgaagtga ccattgattt atcaggactt aatttaacca
      721 ctcaaccacc agggctgcaa aacttcacat ctatcaatct tgataataac caactcacac
      781 attttgatgc aaccaactac gatagactcg taaagctaag tctgaatagt aatgctcttg
      841 agtcaataaa ttttcctcaa ggcagaaatg taagcattac acatatatct atgaataata
      901 atgctctcag aaatattgat atagataggc tttcatcagt tacttatttt agtgcggcac
      961 ataatcaact agagtttgtg caattagaat cttgcgaatg gctgcaatac ctgaatctca
     1021 gccataatca attaactgat attgttacag gtaataaaaa tgaactctta ctgctggatc
     1081 tatcccataa taaactaaca agt
//
LOCUS       DAZFZC010000337         1096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFZC010000337 DAZFZC010000000
VERSION     DAZFZC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.348"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1096)
                     /gene="istA"
                     /locus_tag="ACHKXP_005448"
     CDS             complement(<1..>1096)
                     /gene="istA"
                     /locus_tag="ACHKXP_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HFO7650201.1"
                     /translation="RSLAISPSVVSRYANRAAQLGIKQWPLPTGWDDTKLKHAFLQTQ
                     VKMKKHSLPDWATVHRELRNKCVTLQLLWEEYCERNPGGFYSYNHYCRMYREWLKTTS
                     PSMRQVHKAGEKLFVDYCGPTVGVTDPETGEIRTAQVIVAVLGASSYTWAEATWSQQL
                     EDWVMSHVRCFQWLGGVPELVVPDNLKSATSRACKYDPDVNPTYQQMLEHYNVAVLPA
                     RPRKPKDKAKAEVGVQVVERWIMARIRHEIFYSLASLNQRIRELLERLNNKIMQKLGY
                     SRAELFIQLDKPALKPLPEASYSYTLVKKVRVHADYHVEIDKHYYSVPCSLLGQQLEA
                     WISGELVRLFNQGQEVAVHPRKRTYGYSTRN"
BASE COUNT          265 a          272 c          269 g          290 t
ORIGIN      
        1 gttgcgggta ctgtagccat aagtacgctt gcgcgggtgc acagcaacct cctgcccctg
       61 attgaagagt cttaccagtt ctccggagat ccatgcttcc agttgctggc ctaacagcga
      121 acatggaacc gagtagtaat gtttgtcgat ttccacgtgg taatcggcat gaactctgac
      181 tttcttcacc agggtgtaac tgtaactggc ttcaggaaga ggcttcagtg cgggtttatc
      241 aagctggatg aagagttctg cacgtgaata acccaacttc tgcattattt tgttattcag
      301 tctttccagc aactcccgaa tgcgctgatt aagcgatgca aggctgtaga agatctcatg
      361 cctgattcgg gccatgatcc agcgttcaac aacctgaacg ccaacttcag ctttggcttt
      421 atctttcggt ttacgtggcc gcgcaggcaa aactgcgaca ttataatgct caagcatctg
      481 ctggtaggta gggttaacgt caggatcata cttacatgcc ctggatgtgg cgcttttcag
      541 attgtccgga acaacaagtt caggaacgcc acccaaccac tggaagcagc gaacatgact
      601 catcacccag tcttcaagct gctgagacca ggtggcctct gcccatgtgt aacttgatgc
      661 cccgagaaca gctacgatga cctgagcagt tcttatttct ccggtctcag ggtcggtaac
      721 gccaacggta ggtccacagt aatcaacgaa aagtttttcg ccagctttat gtacctgacg
      781 cattgatggt gaagtggttt tgagccattc acggtacatc cggcagtaat ggttatagct
      841 gtaaaaaccg cctggattac gctcacagta ttcttcccag agtagctgca gcgtcacgca
      901 tttattacgc agttcccggt gtactgtagc ccagtcaggc agagagtgct tcttcatctt
      961 aacctgggtc tgaaggaacg catgttttag ttttgtatca tcccatcctg taggtaaggg
     1021 ccactgcttt atgccaagtt gagccgcccg attagcatat ctggatacaa cggaagggga
     1081 gattgcaaga ctacga
//
LOCUS       DAZFZC010000338         1093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFZC010000338 DAZFZC010000000
VERSION     DAZFZC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.349"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..196)
                     /locus_tag="ACHKXP_005449"
     CDS             complement(<1..196)
                     /locus_tag="ACHKXP_005449"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO7650202.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEI"
     gene            complement(437..1003)
                     /locus_tag="ACHKXP_005450"
     CDS             complement(437..1003)
                     /locus_tag="ACHKXP_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HFO7650203.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSAQAQAVLTGPVDANSSSLLIG
                     ENSFITNSTGTANNTFLLGGGAFNMDSPGSLQFGSFSGVYNSPHSVTLGRDAGQAESK
                     YGVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGSQVSGENNVVSVGAGPEGYG
                     ESVKGAPETHSIVNKTFLSFIFNGLFSC"
BASE COUNT          301 a          271 c          247 g          274 t
ORIGIN      
        1 cgatttctgt gtcggacgaa acaggcgcgg cgtcggggtt gctggatgcc ggaccgaggt
       61 cgatcacatc tgccccctcg gccatcagct tacgcgcctg cgcaatggct gcgtctggcg
      121 ccagataccg gcctccatcg gagaaactgt ccgaggttat gttgacgatg ccgaaaatga
      181 tgagcgattt attcatgggg gcttctataa taataataat cgagcatgag tctcatacgg
      241 atgctcgggt cgaaagggaa tccccaggcg agtaacctgt ttgcggtgat ccattagctg
      301 caggagcaga atagcataca tctggaagca aagccaggaa agcggcctat ggagctgtgc
      361 ggcagcgctc agtaggcaat ttttcaaaat attgttaagc cttttctgag catggtattt
      421 ttcatggtat taccaattag caggaaaata agccattgaa tataaaagat aaaaatgtct
      481 tgtttacaat agagtgggtt tccggtgctc ctttgacact ctctccatat ccctcaggcc
      541 cggcaccaac agataccacg ttattttctc ccgacacctg tgagccatgg cccagagcta
      601 cagagcctga agaattttct attccagccc atcccccaat agcaacactc tgctgtgaat
      661 taagtacctc agcgctctta cctatggcaa caccatattt tgactcagcc tgaccggcat
      721 cccttcccag ggtgacactg tgtggagaat tatacactcc cgaaaaagag ccaaactgaa
      781 gacttcctgg tgaatccatg ttaaaagcgc cacctcccaa aaggaaagtg ttattagctg
      841 taccagttga attcgtaata aaagagtttt ctccaatcag taagctggaa gaattagcat
      901 caacaggccc tgtcaaaaca gcctgagcct gagccgagag cagcagggcc acactcactg
      961 ccagcgcgct tttctttaaa aaagcatcac gtcttttaag cattctgtta ttctcctgta
     1021 ttattacttc accagccaga acggtagtgc ggagcaaaaa tacagtgata agaatcctct
     1081 tttattaatt taa
//
LOCUS       DAZFZC010000339         1085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFZC010000339 DAZFZC010000000
VERSION     DAZFZC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.350"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..670
                     /locus_tag="ACHKXP_005451"
     CDS             53..670
                     /locus_tag="ACHKXP_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7650204.1"
                     /translation="MLASLGGLVSYAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(858..1052)
                     /locus_tag="ACHKXP_005452"
     CDS             complement(858..1052)
                     /locus_tag="ACHKXP_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7650205.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          278 a          230 c          281 g          296 t
ORIGIN      
        1 caataaacac cgaacctgac cctttggaat gggcctttga ggataccagt tagtgctggc
       61 gagcctcggt gggctggttt cctatgcggc aaaggttcat ttcaaagaag caggcaacgc
      121 catgaatgaa ttaattgcga atcatgactt cgactttcgc cagttagtta ccgcagcaga
      181 aggtcaaccg gtaactgaca ccttccagat tgccagggca tttggtaaac gccatcagca
      241 tgtgattagg gctattaaat gtttgagatg ttctgaggaa ttctcgacaa cccatttttg
      301 ggccgtcgag aaaatcaatg acttaggtat ttttgacaag aaacagattt actaccgcat
      361 ggactttagt ggcttcgtta tgctggttat gggatttaac ggggcaaaag ccgatgctgt
      421 taaagaagcc tatatcaatg cgtttaactg gatgtcagca gaactccgta agtacagcga
      481 aagttatgaa gcagaacgta acgccgtaat gctggagtac atgaaagaga aggatgtcgc
      541 cagcatgtca ggccgtctgc tcaatcgctg ggggagaacg aaaaaacctc aattgcttgc
      601 aaagctggaa cgtctggaga gacagggaca gtttttatta ccgggattcg ataaaggtat
      661 tcaagcctga cacattatgc gctgtatcgt cgccgtattc ccgcattaac catgaccgta
      721 gcccgacggg gaattccttc tgcgtgagtg tgcgggaata atcaaaaacg atgcacaccg
      781 ggttttactg tgctgacaga cgcagggtta ccctcatagt cgcttttccg gtgcgatggt
      841 ggaagaaacc gggatgttca tccatcatca ctttggattg atgtatatgc tctcttttct
      901 gacgttagtc tccgacggca ggcttcaatg acccaggctg agaaattccc agaccctttt
      961 tgctcaagag cgatgttaat ttgttcaatc atttggttag gaaagcggat gttgcgggtt
     1021 gttgttctgc gggttctgtt cttcgttgac atgaggttgc cccgtattca gtgtcgctga
     1081 tttgt
//
LOCUS       DAZFZC010000340         1071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFZC010000340 DAZFZC010000000
VERSION     DAZFZC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.351"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..873
                     /locus_tag="ACHKXP_005453"
     CDS             <1..873
                     /locus_tag="ACHKXP_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7650206.1"
                     /translation="LEEEIRADRVSSESRQWLAQCGLTVEQLARQVEPEYTPARKVHF
                     YHCDHRGLPLALISEEGNTVWSAEYDEWGNLLNEENPHHLHQPYRLPGQQHDEESGLY
                     YNRHRYYDPLQGRYITQDPIGLEGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDD
                     SYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAA
                     ITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASV
                     MTLACRTWQECWVN"
     gene            885..>1071
                     /locus_tag="ACHKXP_005454"
     CDS             885..>1071
                     /locus_tag="ACHKXP_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO7650207.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEFNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKKFIDI"
BASE COUNT          301 a          195 c          290 g          285 t
ORIGIN      
        1 ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa gccgtcagtg gcttgcgcag
       61 tgcgggctga cggtggagca actggccaga caggtggagc cggaatacac accggcgcga
      121 aaagttcatt tttaccactg cgaccaccgg ggcctgccgc tggcgctcat cagcgaagaa
      181 ggcaatacgg tgtggagcgc ggaatatgat gaatggggca acctgctgaa tgaggagaac
      241 ccgcatcacc tgcaccagcc ataccgtctg ccggggcagc agcatgatga ggaatcaggg
      301 ctgtactata accgtcaccg gtactacgat ccgttgcagg ggcggtatat tacacaagac
      361 cccattgggc tggagggggg atggaacctg tataattacc cactgaatcc gataataagg
      421 atggatcctt tgggtttgta taatttatat caattattat atgatgtttg gcatgatgat
      481 tcatatggaa catcatcaat tgatattact ggcagtggag atctaatatc attaggtggt
      541 catgcaggac ttggcgttgc gtttgctaaa aagaaaggtg aaatgttatc tgatatttgt
      601 atttatgcta cagcatgcgg acatgcagga attggtggtg ggataaatgc ggctatcaca
      661 tattctgaga ccaagtcttt acctacatcg ggagtcagca attcagtagg tgtaacggtt
      721 ggcggcggag ttgggggaca ttttgcgtat acttatgtag tggatgttga taatccagaa
      781 tcatcgacag aatctgttgg tatcggtgca ggtgttgacg cttcagttat gactctggct
      841 tgtagaacgt ggcaagaatg ctgggtcaat taacaggagg aaatatgaaa aaatatatat
      901 ttttagtttt atttttattg tgtggttgtt ctccagtgga catagagcca gctagacaag
      961 aatttaatat ctttcgtggt ttatatcaga cagggaaata cagtgatata tataaaatca
     1021 cttcaagtga ttttcagact gccacatctg aaaaaaaatt tatcgacata a
//
LOCUS       DAZFZC010000341         1066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFZC010000341 DAZFZC010000000
VERSION     DAZFZC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.352"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          375 a          217 c          166 g          308 t
ORIGIN      
        1 ccttatcagg aaggatcaac aattaatcga atgatataaa ttatgtgaga taacgtttta
       61 tctatcaaat ttagaacaca tctacttcat caaacaaaat ccttataaaa gttattaatc
      121 accgatgtat aatgtgtaaa atacattatc attaaatgtc aacaaaaaaa tcatatttct
      181 taaaaatcat tcaaaatcaa taaagtagaa gaacttcatc tctttagcaa acgccattaa
      241 caattgcctt tccatttagt taatcgtttt gttttcttta agattagtcc tattagacaa
      301 atagcaaaat ggaataacta cagcactaag acgttccgct atatagctat tacctgttcc
      361 cctgacataa ggagcagata tacctcaacc ccctattccc cagtgctgac cgggagcggc
      421 ggcggtataa aaaggaacaa ggaacaaatg gcagaacaag caaccatgtt tgtctccccc
      481 gcataaccgt accattcgca ttaagaaata ttcccatatc tatcccgcaa aagaggcccc
      541 gtcatacatc aagtccgttt tactgggcat cagattatcg ccgtaattag ctcagtgaaa
      601 gccgggtaaa cggttagcaa gctcagtggc tcaaaagtgc gtagcgctaa gatgggcctg
      661 gttacacctg ccctttgctg cagatcgtgt tgatcggtgt gatgccgcca cacgtttaag
      721 tcaatctgga aaactcacaa tgcgctcttc tataaagagg actctttgat aaagtaaccc
      781 gaaaagggaa agctaactga tatcaatgta ccaccttagt aatcatccct tatatacaga
      841 caaaaagaag ttgcctgaaa gccaggtatc taaactaatc actaatggta tattttatca
      901 gcacaataaa aattttttct gaatgctatt attaatgtaa ataattacaa ctttttcgta
      961 ggcacaaaaa ctattgctga tgaatcaatg aaaatctatt gttcgtcatt agaggaatat
     1021 ttcctctaat tttaaaaaaa ataactttct atacatttaa aactct
//
LOCUS       DAZFZC010000342         1057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFZC010000342 DAZFZC010000000
VERSION     DAZFZC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.353"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            151..339
                     /gene="dicB"
                     /locus_tag="ACHKXP_005455"
     CDS             151..339
                     /gene="dicB"
                     /locus_tag="ACHKXP_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7650208.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            336..524
                     /locus_tag="ACHKXP_005456"
     CDS             336..524
                     /locus_tag="ACHKXP_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7650209.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            620..>1057
                     /locus_tag="ACHKXP_005457"
     CDS             620..>1057
                     /locus_tag="ACHKXP_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFO7650210.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIICEDKPGIPRPALDSWDAEYMQENRWDEDSASFVPVETESDLM
                     NVTFDKLAPEVQNALMVKFDTCENITVDMVISAQELLQEDMATF"
BASE COUNT          305 a          229 c          245 g          278 t
ORIGIN      
        1 ggtgaatgcg gctgagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
       61 tctctgtatc cggcgttaat tgttaactgg ttaacgtcac ctggaggcac caggcactgc
      121 atcacaaaat tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg
      181 tctgaaggtt gttttgatat tggtgttctg ctcagtaacc gggagtttac tgaagatgcc
      241 attaatatga ggaaatatga gccttatctg ctcaatgata attccatact ttcccgaatt
      301 gctcttcttg aacttggtat tttcggagaa cgtcaatgac ttcagcattt gcactgatga
      361 tgacggtttt tcttataacg ggtgaatcac agaatgtgat taccggaatt tatgcaagta
      421 aagaatcctg cctccaggca agagacgagc aaaaaatttc tggtgaatgc ctcccgctaa
      481 aaaaagtatc gctgtacctg aataacgaaa caccggctgg ataaccctcc agccatatta
      541 acaccatacc aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct
      601 gtcatgaggt taaaacaaaa tgagtaaagt ctttatttgc gccgccattc ctgacgaact
      661 ggcaacaagg gaagaaggcg ctgtggctgt agccacagct attgaagctg gcgacgaacg
      721 ccgtgctcga gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg
      781 cgcttataaa tttattatct gcgaggataa acctggcata ccccgccctg ccctcgattc
      841 atgggatgct gaatatatgc aggaaaaccg ctgggatgag gattctgctt cttttgtccc
      901 ggttgagact gaatccgatc tgatgaacgt cacttttgac aagctggccc ctgaagtaca
      961 gaacgctctc atggttaagt tcgacacatg tgaaaacatc accgttgata tggttattag
     1021 cgcacaggaa ttgttgcagg aagacatggc aacattc
//
LOCUS       DAZFZC010000343         1043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFZC010000343 DAZFZC010000000
VERSION     DAZFZC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.354"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1043
                     /locus_tag="ACHKXP_005458"
     CDS             <1..>1043
                     /locus_tag="ACHKXP_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO7650211.1"
                     /translation="SGDKSDIIYKVDGLSIIARNFFTLLSGNFKPDWRWDVSKETFTK
                     EKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREK
                     GWSLLCELLKHEEMASSAYKHKNNKVLYDLLNTRDMILNELHQHVFLKDDAITPCIFL
                     GDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYTARL
                     ANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKN
                     PTDPEELANIFNKKHKEHMDDPLFHLIRSNTLKPTPVYANYFDNTTDFRPARERIFIC
                     GETLKGEDPSKYI"
BASE COUNT          354 a          169 c          199 g          321 t
ORIGIN      
        1 tctggagata aatcagatat aatttataaa gttgatgggc tttctatcat agcgagaaat
       61 ttctttactc ttcttagtgg taatttcaaa cctgactggc gttgggatgt ctctaaagag
      121 acgtttacta aggaaaagtt tgatagctat gttaaatctg tgttttcaaa aatagacttc
      181 tataaacagt gtggtgtgat taacccacaa aatgccaaca cggcttattt tggcgataca
      241 gatggaagag taggcgctgt actttatgca ttgctcgttt cagggcatat aggcatcagg
      301 gaaaagggtt ggagtttatt atgtgaatta ttaaaacacg aagagatggc ttcatctgca
      361 tacaaacata aaaataataa agtgctctac gatttattaa atacgagaga catgatcttg
      421 aatgagttgc atcaacatgt ttttttaaag gacgatgcga taaccccttg tatcttcctt
      481 ggggatcata caggcgatag atttagtact atttttggtg ataaatatat ccttactttg
      541 ctaaattcta tgcgaaatat ggagggtaat aaagatagcc gtattaataa aaatgtcgtc
      601 gttctggctg gaaatcatga aatcaatttt aatggtaact atactgcacg actagcaaat
      661 cataaactct ctgccggtga tacgtataac ttaattaaaa cgctcgatgt ttgtaattat
      721 gactcagaaa ggcaagtttt aaccagtcat cacggaataa taagagatga agaaaaaaaa
      781 tgttattgtc tcggtgcttt gcaggttcca tttaatcaga tgaaaaatcc tactgatcct
      841 gaggaattag ccaatatttt taataaaaaa cataaagaac acatggatga tcctctattc
      901 catctaatcc gaagcaatac tttgaaaccc acacctgtat atgctaatta ttttgataac
      961 actactgatt tcagaccagc acgtgaacgt atcttcatat gtggggagac actcaaaggt
     1021 gaagacccca gcaaatacat tag
//
LOCUS       DAZFZC010000344         1039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFZC010000344 DAZFZC010000000
VERSION     DAZFZC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.355"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..230
                     /locus_tag="ACHKXP_005459"
     CDS             <1..230
                     /locus_tag="ACHKXP_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO7650212.1"
                     /translation="TISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGG
                     CRLIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            261..776
                     /locus_tag="ACHKXP_005460"
     CDS             261..776
                     /locus_tag="ACHKXP_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO7650213.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
BASE COUNT          322 a          177 c          205 g          335 t
ORIGIN      
        1 gtactatcag caatgaggct gtaatggatc gcatttattg tgttattgga acaattcatg
       61 ttgatataca tatgttgcgc aatgccaaaa aagatgacat tatcgattca actggatatc
      121 atctttttgg tgggtgccgg ttaatacgca ataatacgac tatagcatac ggaagcattg
      181 taaaaatcaa cgaagatttt tattttactg ttagtttagt atgtgattaa tcaggaataa
      241 gttatatagg gagatgtatc atgtcgtcat cgttatcagg aataatactc aatacttcgc
      301 tgacaagcca ccatatgtca tggggcaagt ttagcagaac tcttcatgta ttgaatgaag
      361 atatcgtaga tgaaattcag cttaaagccc gaacacaaca acgccaccat cgagtatatt
      421 cagaaatggc ggattactcc gttgtctcct ttcggggcaa agaatattct gttcgtttta
      481 ttaaagatta caaaacagat aattatgtgt acaaagtaca aaagatgact caggaaagtg
      541 ggtgtctctc aagattattt agctttcttt ctggcggtgt gactaaggct ttggaaagta
      601 aactaaatga gaggcatatc actccacttt ctccaacctg gtttcctcga actccactgg
      661 agggggtctt aacggaaaga ggattatctt ctttgttacg tcgcgtacag tctactgaaa
      721 accttgatta tagtaatgct atgatatcaa actctttctc taactccgta gtgtgattat
      781 catttgagct ttcctcctga cttaactcca gaataattgt gattctggag tttttctgtt
      841 cgttttatgt cgataataca tcttttagaa aaatacagga tgaatattca gagtgagttg
      901 gctggcagaa gtcagagatt ctgtaatgag gaggggattg agctgaatgg atgaactcct
      961 tccagctcat aatttatcag gataaaacat cttacgacaa taaactaatt acttcattct
     1021 ttaataaata accgttttc
//
LOCUS       DAZFZC010000345         1037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFZC010000345 DAZFZC010000000
VERSION     DAZFZC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.356"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(137..952)
                     /locus_tag="ACHKXP_005461"
     CDS             complement(137..952)
                     /locus_tag="ACHKXP_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HFO7650214.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          307 a          240 c          212 g          278 t
ORIGIN      
        1 atttatgagt aaaggattat gtccacgata agcacctggg tcgattcctg ggaggcggcc
       61 atgagggtag ggaagcgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
      121 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
      181 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
      241 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
      301 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
      361 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
      421 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
      481 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
      541 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
      601 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
      661 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
      721 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
      781 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
      841 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
      901 tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc
      961 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1021 gtgcaatgta acatcag
//
LOCUS       DAZFZC010000346         1019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFZC010000346 DAZFZC010000000
VERSION     DAZFZC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.357"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..564
                     /locus_tag="ACHKXP_005462"
     CDS             <1..564
                     /locus_tag="ACHKXP_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO7650215.1"
                     /translation="LKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSA
                     MIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSY
                     PFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGN
                     FKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            536..919
                     /gene="yhhH"
                     /locus_tag="ACHKXP_005463"
     CDS             536..919
                     /gene="yhhH"
                     /locus_tag="ACHKXP_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO7650216.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          367 a          147 c          231 g          274 t
ORIGIN      
        1 ctgaaggggg gatggaacct gtatggatat caattgaatc cgatatcaga catcgacccc
       61 ctgggtttat ctatgtggga ggatgcaaaa tcgggggcat gtactaatgg tctttgcggc
      121 acactatccg ctatgatagg tccagataaa tttgattcta tagatagcac cgcatatgac
      181 gccttaaata aaataaatag ccaatctatt tgcgaagata aagagttcgc tggtttaata
      241 tgtaaggata atagtggcag atatttctca acagcaccta accgaggaga aagaaaagga
      301 tcatatccat tcaatagccc ttgccctaat ggtactgaga aagtatcagc ttatcatact
      361 catggtgcag atagtcatgg agaatattgg gacgaaatat tttcaggtaa agatgagaaa
      421 atagttaaaa gtaaagataa caatatcaag tcattttatt taggtacgcc cagtggtaat
      481 tttaaagcaa tagataacca cgggaaggaa ataacaaaca gaaaaggatt acctaatgtc
      541 tgcagagttc atggtaatat gtaaaaaaat attgtttagg aactgtgtca ttgtatcttt
      601 gtttgttttt acatacaaca catgggcgca gtgtaataat aatattaaaa taatgcgcaa
      661 gtatgaaagt gaaggtaaat ataccgttag aaatttggtt aaaaataaag cgatagcatt
      721 ggaattagct gagatatatg ttaagaatcg ttatggacag gatgccgcag aagaagaaaa
      781 accatacgaa atcactgagt taacaacaag ttgggttgtt gaaggtacca ttcactcaga
      841 ccaaattgct ggtggggttt ttattataga aataggcaaa aatgatggga gaattctgaa
      901 ttttggccac ggaaaataag tatgtaagga taatcaaaca tcgtgtgcgc tgatggcaga
      961 gtaggtggag gactccagac agtcaaacga tagaaaaaga tagcctttat ggaggttcc
//
LOCUS       DAZFZC010000347         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFZC010000347 DAZFZC010000000
VERSION     DAZFZC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.358"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..274
                     /gene="cbtA"
                     /locus_tag="ACHKXP_005464"
                     /pseudo
     CDS             1..274
                     /gene="cbtA"
                     /locus_tag="ACHKXP_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            271..465
                     /locus_tag="ACHKXP_005465"
     CDS             271..465
                     /locus_tag="ACHKXP_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7650217.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            511..591
                     /locus_tag="ACHKXP_005466"
                     /pseudo
     CDS             511..591
                     /locus_tag="ACHKXP_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(880..>1002)
                     /locus_tag="ACHKXP_005467"
     CDS             complement(880..>1002)
                     /locus_tag="ACHKXP_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO7650218.1"
                     /translation="CRLNGIDPEAYLRHILSILPEWPSNRVDELLPWNVVLTNK"
BASE COUNT          283 a          241 c          250 g          228 t
ORIGIN      
        1 atggcctcac actgaatgac acaccttttg ccgatgaacg tgtgattgag cagcatattg
       61 aggcaggcat ttcactgtgt gatgcggtga actttctcgt ggaaaaatac gcgctggtgc
      121 taccgaccag ccgggattca gcgcctgtac ccgctctcag ttaataaaca gcatcgatat
      181 cctccgggct cgcagggcga ccggcctgat gacccgcgac aattacagaa cggtaaataa
      241 cattaccctg ggtaagtatc cggaggcgaa atgatgacac tggaagccga cagcgttaac
      301 gtacaggcgc tggatatggg gcacattgtc gttgacattg atggtgttaa tatcactgaa
      361 ctgattaata aggccgctga aaacggttat tcactccgcg tggtggatga ccgtgactct
      421 accgaaacac cggcaactta tgccagccct caccagttgc tgtaagacaa tgcagtgatg
      481 cagggtatag tgccattaac cacagaaaca gcactgttgt cctgtattaa gcaggcaaga
      541 gaagatgtcc ttaccctgcg ccatctgcag cttctgcacc agaaccggtg aatctcactc
      601 aacaggcttc atttcccctg acgccagcct gaatacagct ggcgttttca tttataaaca
      661 gaaaggaaaa ccgataataa tggaatggac gccccgacca tgaagcaacg gtaggatatt
      721 actgataaac cttccagtgg aggtgcgtta tggccgtttt aacgattagc attgacctga
      781 caaaaaatga attccaaatc cacggtctgg gtaggaacag aaaaatctaa gctcagaaaa
      841 cggattaaag taagcgtcaa cggagcaccg tattgacgct tatttattgg tgagaactac
      901 gttccatggc aggagttcgt caacacggtt ggagggccat tccggcagta tgctcagaat
      961 atggcgcaga tacgcttccg gatcgatacc gttcagacgg ca
//
LOCUS       DAZFZC010000348          999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFZC010000348 DAZFZC010000000
VERSION     DAZFZC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.359"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..494)
                     /gene="yhaB"
                     /locus_tag="ACHKXP_005468"
     CDS             complement(<1..494)
                     /gene="yhaB"
                     /locus_tag="ACHKXP_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO7650219.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYHGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCW"
     gene            complement(750..968)
                     /gene="tdcR"
                     /locus_tag="ACHKXP_005469"
     CDS             complement(750..968)
                     /gene="tdcR"
                     /locus_tag="ACHKXP_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO7650220.1"
                     /translation="MSKFSNFIINKPFSVINNAACHIFSRYLLENKHLFYQYFKISNT
                     CIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          309 a          179 c          183 g          328 t
ORIGIN      
        1 tgccaacaat ctgaagtaat gctggtttta ttaaagtgca caaggggggt taagatggct
       61 tcttttcggg tgaattttgc acaatactta gaaatcgtct ccttaataca ttccagctct
      121 tttttattga cggctgagca agtacattgt tcaccaaaag gtgcaaaagt gcctacatct
      181 aatgtttgta tgatataatc atcatgttct cctgtagaaa tatctataat gaatggactc
      241 atattttccg gttggattcg gatcatgaca gaaggaacat cgccatgata gaaattcaat
      301 gcagttattg cagataatac tctccgcagt tcattactta tactgtaaat tttattaacg
      361 tcattggtgg agacaatttc tgcaaacttt ctttttaccg tccaataatc aatatttcta
      421 ttgtgattgt taaccactgc gtgcattggc gggattgaag ggtgaaaaat atgcgcaatt
      481 gggaagcctt tcattgatgc ctgccttgtc atcatgacaa acaataatag atgcttattt
      541 tcctcgcagg gttctttcgc actatcaaaa aacgctcatt tacccggcgt tacagtaaac
      601 gaagcaacat tctgtggcct acgccgatca aaagctctgt gaaaatttcc cttgttgtct
      661 ttgtctgacc ttatcgcggc gatcagcaat tattcgtttt gtgatcctca acacaaaatg
      721 ctattcgtcg cctatgaggg gagaagaatt tatggaaaac gattgcattc acaaaatgac
      781 gtcctgtcag aagagaaaaa gttgacgtta atcaattgtt cgagatgatc aatacatgta
      841 ttcgaaattt taaaatattg ataaaataaa tgtttgttct ccaacaaata gcgtgaaaaa
      901 atgtgacatg ccgcattatt tatcactgaa aatggtttat ttataataaa atttgagaac
      961 ttgctcatta aatttaactc aaattttgcc tggtaatta
//
LOCUS       DAZFZC010000349          994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFZC010000349 DAZFZC010000000
VERSION     DAZFZC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.360"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..448
                     /gene="yhaJ"
                     /locus_tag="ACHKXP_005470"
     CDS             <1..448
                     /gene="yhaJ"
                     /locus_tag="ACHKXP_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO7650221.1"
                     /translation="APDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTR
                     VKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKD
                     IAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFNGK"
     gene            complement(499..855)
                     /locus_tag="ACHKXP_005471"
     CDS             complement(499..855)
                     /locus_tag="ACHKXP_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7650222.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLFFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          299 a          204 c          246 g          245 t
ORIGIN      
        1 cgcgccggat atgcattttc gttcctcgtc ggagatcaac tcgcgcaagc tctatacgtt
       61 aatgaacgtc tacgttgccg cgcctgatca cccgattcat caggagccgg aaccgttatc
      121 tgaagtgacg cgtgtgaaat atcgtggaat tgcggtggcg gataccgctc gtgagcgccc
      181 ggtgttgacc gtacagctgc tggacaaaca gccgcgctta acggtgagca cgattgaaga
      241 taaacgtcag gcattactgg cggggcttgg cgtggcgacg atgccgtatc ctatggtcga
      301 aaaagatatt gcggaagggc ggttgcgtgt cgtcagcccg gaatcgacca gcgagatcga
      361 tattattatg gcctggcgtc gtgacagtat gggggaagcg aaatcctggt gtctgcggga
      421 aattcccaaa ctttttaacg gaaaataata tttattattg ttaataaaaa ggggcgaaat
      481 atcgccccaa ttctaagatt aatttgaacc aaacttcgga tcgtttccgt aacgattgct
      541 gccagaagta ccttctgtgc agaaaaaaaa cagaagcacg agccagccaa taaacggaac
      601 aaaaaagagc aaggcccagg cacctgaacg gtcggtatcg tgtaagcgac ggatagcaag
      661 cgcaaggacg ggaaggaagg ttgccagcaa ataaagcata gacagatatg gaagctccag
      721 acctaaaatc aattgaatga cattgataat agcgccgacg atggcgttaa tcagagtaaa
      781 catccagtac tctttacgac gtgcgcgacc agagaaacca acataatttt ttaatacgga
      841 caaataccac tgcataaaaa catcccttta attaaaagtg atgaaagtct attccgggta
      901 ttgtgtaaat gctattcgta tctttctttt tcggatgtta taaaggtttt gatatttaag
      961 ataaatacat ggttattttt tataggtaat ttat
//
LOCUS       DAZFZC010000350          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFZC010000350 DAZFZC010000000
VERSION     DAZFZC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.361"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>992
                     /locus_tag="ACHKXP_005472"
     CDS             <1..>992
                     /locus_tag="ACHKXP_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7650223.1"
                     /translation="AAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSV
                     WPKSIRGSTPKIKGTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWE
                     KDSPESVFYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRS
                     ITFHIWTAYSPFTTWIQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLM
                     DKVVRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAQGEEAFLVDKIIIMGRPDEEET
                     LLRVDAAINKKYCHADGTEMTISRVCWDSGGIDGEIVYQRSKKHGVFRVLPVKGA"
BASE COUNT          237 a          243 c          299 g          213 t
ORIGIN      
        1 gcagccgcca aaaactaccg tgaaaaatcc gtggacgtgg tctgctatga cgagctttcc
       61 tcgttcgagc cggatgtcga aaaagagggc tcgccaaccc tgctggggga taagcgtatt
      121 gagggctctg tatggccaaa atccattcgc ggctcgacgc ctaaaatcaa aggtacctgc
      181 cagatcgaaa aagcggccaa cgagtcggcg catttcatgc gtttctatgt gccctgcccg
      241 cactgtgggg aggcgcagta tctgaaattt ggcgatgaat ccacgccttt tggccttaaa
      301 tgggagaagg acagccccga aagcgttttc tacctctgtg aacatcatgg ctgcgtgatc
      361 catcagtctg agcttgacca gagtaacggg cgctggatct gtgaaaacac gggtatgtgg
      421 acccgtgacg gcctgatgtt tttcagcgcc cggggggatg aaattccgcc gccgcgctcc
      481 atcacgttcc atatctggac ggcgtacagt ccgttcacca cctggataca gatagtctat
      541 gactggctgg atgcactgaa agatcccaac ggcctgaaaa cctttgtgaa caccacgctg
      601 ggcgagacct gggaagaggc cgtgggcgaa aaactcgatc accaggtact gatggataag
      661 gtggtgcgtt acacggcggc ggtgcctgcc cgggtggttt atctgacggc gggcattgac
      721 tcgcagcgaa accgttttga gatgtatgtc tggggatggg cacagggaga ggaagccttt
      781 ctggtggata aaatcatcat tatgggccgt cccgatgagg aagagacgct gttacgtgtg
      841 gatgcggcga tcaacaaaaa atactgccat gcagacggaa ccgaaatgac catttcccgt
      901 gtctgctggg actccggggg gatcgatggt gaaattgtct atcagaggtc aaaaaaacac
      961 ggtgttttcc gggtgctgcc ggtaaaaggc gc
//
LOCUS       DAZFZC010000351          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFZC010000351 DAZFZC010000000
VERSION     DAZFZC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.362"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>992
                     /locus_tag="ACHKXP_005473"
     CDS             <1..>992
                     /locus_tag="ACHKXP_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7650224.1"
                     /translation="AAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSV
                     WPKSIRGSTPKIKGTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWE
                     KDSPESVFYLCEHHGCVIHKSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRS
                     ITFHIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLM
                     DKVVRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEET
                     LLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGA"
BASE COUNT          234 a          239 c          301 g          218 t
ORIGIN      
        1 gctgccgcca aaaactaccg tgaaaaatcc gtggacgtgg tctgctatga cgaactttcc
       61 tcgttcgagc cggatgtcga aaaagagggc tcgccaaccc tgctggggga taagcgtatt
      121 gaggggtcgg tgtggccaaa atccattcgc ggctcgacgc ctaaaatcaa aggcacctgc
      181 cagatcgaaa aagccgctaa cgagtcggcg cattttatgc gtttttatgt gccctgcccg
      241 cactgtgggg aggcgcagta tctgaaattt ggcgatgagt ccacgccttt tgggcttaaa
      301 tgggagaagg acagccccga aagtgttttc tacctctgtg aacatcatgg ctgcgtgatc
      361 cataagtctg aactggacca gagcaacggg cggtggatct gtgaaaacac gggcatgtgg
      421 acccgtgacg gtctgacgtt tttcagcgcc cggggtgatg aaattccgcc gccgcgctcc
      481 atcacgttcc atatctggac ggcgtacagt ccgttcacca cctgggtaca gattgtctat
      541 gactggctgg atgcactgaa agatcccaac ggcctgaaaa cctttgtgaa caccacgctg
      601 ggcgagacct gggaagaggc cgtgggcgaa aaactcgatc accaggtact gatggataag
      661 gttgtgcgtt acacggcggc ggtgccttcc cgggtggttt atctgacggc gggcattgac
      721 tcgcagcgaa accgttttga gatgtatgtc tggggatggg caccgggaga ggaagccttt
      781 ctggtggata aaatcatcat tatggggcgt cccgatgagg aagagacgct gttacgtgtg
      841 gatgctgcga tcaacaaaaa ataccgccat gcggatggca ccgaaatgac catttcccgt
      901 gtctgctggg acaccggggg aatcgatggt gaaattgttt atcagagatc aaaaaaacac
      961 ggtgttttcc gggtgctgcc ggtaaagggc gc
//
LOCUS       DAZFZC010000352          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFZC010000352 DAZFZC010000000
VERSION     DAZFZC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.363"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..279
                     /locus_tag="ACHKXP_005474"
     CDS             <1..279
                     /locus_tag="ACHKXP_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7650225.1"
                     /translation="LAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINA
                     GDRKDACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWDIDR"
     gene            500..613
                     /locus_tag="ACHKXP_005475"
     CDS             500..613
                     /locus_tag="ACHKXP_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650226.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            615..926
                     /locus_tag="ACHKXP_005476"
                     /pseudo
     CDS             615..926
                     /locus_tag="ACHKXP_005476"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            868..>987
                     /locus_tag="ACHKXP_005477"
     CDS             868..>987
                     /locus_tag="ACHKXP_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7650227.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDWQTPL"
BASE COUNT          242 a          224 c          292 g          229 t
ORIGIN      
        1 ctggcatggg tggagaaaaa catccgggtg ccgctgaccg aaccccagaa agcggggatc
       61 gcgtcattct gtccgtacaa cattggcccc ggtaagtgct tcccgtcgac gttttataaa
      121 cgaattaatg caggcgatcg aaaagatgcc tgtgaggcga ttcgctggtg gattaaggac
      181 ggtggcagag actgccgtat ccgttcaaat aattgctacg gtcaggtctc acggcgtgac
      241 caggagagcg cgctggcgtg ctgggacatc gacagatagc agaatatttt cctgaaaaat
      301 gacgttggcc aacgcgggtg gataacacga aatcctgaaa actggtaaaa cctaagtgaa
      361 taaaagtaaa aaccccgttt gttggcagca agcggggttt tgtgttttct gaccttgagt
      421 aaggcaaggg agaaattatg ggtagggagg tactttccct gtgaggaagt ataaaagatt
      481 ctttctgagg ttgtccatta tgaaaggcat tgaagtggag acgccagcca gtctggattt
      541 aacaagagcg gcagcttttg ccattcgtat tgtggccatt gctgttctgg tctgggcaat
      601 ccgttggtgg tgatatgaac cgtgttctgt gcgtggttat cattgtcctg ctggtggcct
      661 gtggtgcgct tagtctgggg ctgaatcatt accgtgatca cgccatcatc tacaaagagc
      721 agcgcgataa aaaagccagt gagctggagc tggcgaacgc gacaattact gatatgcaga
      781 tacgccagcg tgatgtcgct gcacttgatg ccagatactc gagggaatta gccgatgcga
      841 gagctgaaaa tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc ctgcggatca
      901 acgccacctg ctccggtacc gtgcgtgaag ccaccggcac ctccggcgtg gataatgcaa
      961 ccggcccccg actggcagac accgctg
//
LOCUS       DAZFZC010000353          980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFZC010000353 DAZFZC010000000
VERSION     DAZFZC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.364"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..78
                     /locus_tag="ACHKXP_005478"
     CDS             <1..78
                     /locus_tag="ACHKXP_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7650228.1"
                     /translation="RRISQDISTRDEGGDGKVVVIGRQA"
     gene            324..>980
                     /locus_tag="ACHKXP_005479"
     CDS             324..>980
                     /locus_tag="ACHKXP_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7650229.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     G"
BASE COUNT          251 a          237 c          308 g          184 t
ORIGIN      
        1 cggcgaatct cccaggacat cagtacccgt gatgaaggcg gtgacgggaa ggtggtggtt
       61 atcgggcggc aggcataaaa gcgaaaaaat cccgcagtga ccgaaggctg cgggaacaga
      121 aaatgaagat taaccacagg gggttttgtt tttattggcc cgaaaaaact gtaacgcccg
      181 ggaatgatat ctgccacggg ggcgtacaga aaatgtgaag aaattcagaa attttattcc
      241 gtcatgacac aggcaccctc cggggtgcct gtcgtttttg gggcataaac agattcagac
      301 atcagacagg agagggggac cgagtgggta aaggtggcgg cagggcgcac acgccggtag
      361 aggcaaagga caatcttaag tccacgcaga tgatgagcgt gattgacgcc attggtgaag
      421 ggccgattga aggtccggtg aaggggctgc agagtatcct ggtgaacaaa accccgctga
      481 cggacacgga cggtaatcct gtgatacatg gtgtgacagc ggtctggcgc gccggggagc
      541 aggagcagac accgccggaa ggttttgagt catccggctc tgaaaccgca ctgggcgtgg
      601 aagtgacgaa ggcaaagccg gtgacgcgca ccatcacgtc agcgaacatt gaccgtctgc
      661 gggtcacctt cggggtgcag tcactggtgg agaccacctc aaagggcgac cgtaatccct
      721 cttctgtccg actgctgatt cagttgcagc gtaacggtaa ctgggtgacg gaaaaggatg
      781 tcaccattaa cggcaagacc acctcgcagt ttctggcgtc ggtgattctg gataatctgc
      841 ctccccgtcc ttttaacatc cggatggtcc gggagacagc ggacagcacc tcggaccagc
      901 tgcagaataa gacgctctgg tcgtcataca ccgaaatcat cgatgtgaaa cagtgctacc
      961 cgaacacggc gattgtgggg
//
LOCUS       DAZFZC010000354          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFZC010000354 DAZFZC010000000
VERSION     DAZFZC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.365"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>968
                     /locus_tag="ACHKXP_005480"
     CDS             <1..>968
                     /locus_tag="ACHKXP_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7650230.1"
                     /translation="SDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEGG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEIRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKL"
BASE COUNT          240 a          278 c          296 g          154 t
ORIGIN      
        1 agcgacgggc gggtgacaga acagcttaac ccggcaggct taagctacac gtatcagtat
       61 gagaaagacc gcatcaccat caccgacagc ctggaccgcc gtgaagtgct gcacacgcag
      121 ggcgaaggcg ggctgaagcg ggtggtgaaa aaggaacacg cggacggcag cgtcacgcag
      181 agtcagtttg acgcggtggg caggctcagg gcacagacgg atgccgcagg caggacgact
      241 gagtacagcc cggatgtggt gacgggcctc atcacgcgca tcaccacgcc ggatggcagg
      301 gcatcggcgt tttactataa ccaccacagc cagttaacgt cagccaccgg gcctgacggg
      361 ctggaaatac gccgggaata tgatgaattg ggccgtctga ttcaggaaac tgcccctgac
      421 ggcgatatca cccgctaccg ttatgataat ccacacagtg acttaccctg cgcaacggaa
      481 gatgccaccg gcagccggaa aaccatgacg tggagccgtt acggtcagtt gctgagcttc
      541 accgactgtt ccggttatgt aacccgttat gaccatgacc gttttggtca ggtgacggcg
      601 gtgcaccgcg aggaagggct gagtcagtac cgcgcatacg acagccgtgg acagttaatt
      661 gccgtgaaag acacgcaggg ccatgaaacg cggtatgaat acaacgccgc cggtgacctg
      721 accaccgtca ttgccccgga cggcagcaga aacgggacac agtacgatgc gtggggaaag
      781 gccatctgta ccacgcaggg cgggctgacg cgcagtatgg aatacgatgc tgccggacgg
      841 gtcatccgcc tgaccagtga aaacggtagc cacaccacct tccgttacga tgtactcgac
      901 cggctgatac aggaaaccgg ctttgacggc cgcacacagc gttatcacca cgacctgacc
      961 ggcaaact
//
LOCUS       DAZFZC010000355          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFZC010000355 DAZFZC010000000
VERSION     DAZFZC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.366"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..374)
                     /locus_tag="ACHKXP_005481"
     CDS             complement(<1..374)
                     /locus_tag="ACHKXP_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650231.1"
                     /translation="MSAFCASFCRFSACSINDLASRGDIRRESASAKSIRLSVCSRRA
                     SYPAISVRNPYSLSSACRSLNSCRVISAASAFPARSVFSVFSSFSLAAIAAERRFAAS
                     VRLFSFSFCCALSVILYAVISLS"
     gene            271..>968
                     /locus_tag="ACHKXP_005482"
     CDS             271..>968
                     /locus_tag="ACHKXP_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO7650232.1"
                     /translation="MLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEY
                     ERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLN
                     TARNALTRAEQQLTQQKNTLDGKMIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTS
                     AVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLL
                     DARNKITSAESAVNS"
BASE COUNT          298 a          225 c          252 g          193 t
ORIGIN      
        1 gacaaggaaa tcacagcgta caaaatgacg ctcagcgcgc agcagaaaga gaatgagaat
       61 aagcgtactg aagccgcaaa acgcctctct gcggcgattg ctgcaaggga aaaagatgaa
      121 aacacactga aaacactccg tgccggaaac gcagatgccg ctgatattac acgacaggag
      181 ttcagactcc tgcaggcaga gctgagagaa tacggattcc gtactgaaat cgccggatat
      241 gatgccctcc ggctgcatac agagagccgg atgctctttg ctgatgctga ttctcttcgt
      301 atatctcccc gggaggccag gtcgttaatc gaacaggctg aaaaacggca gaaggatgcg
      361 cagaacgcag acaagaaggc cgctgatatg cttgctgaat acgagcgcag aaaaggtatt
      421 ctggacacgc ggttgtcaga gctggaaaaa aatggcgggg cagcccttgc cgttcttgat
      481 gcacaacagg cccgtctgct cgggcagcag acacggaatg acagggccat ttcagaggcc
      541 cggaataaac tcagttcagt gacggaatcg cttaacacgg cccgtaatgc attaaccaga
      601 gctgagcaac agctgacgca acagaaaaac acgcttgacg gcaaaatgat agtttcccct
      661 gaaaaattcc cggggcgttc atcaacaaat cattctattg ttgtgagcgg tgatccgaga
      721 tttgccggta cgataaaaat cacaaccagc gcagtcatcg ataaccgtgc aaacctgaat
      781 tatcttctga cccattccgg tctggactat aaacgcaata ttctgaatga ccggaatccg
      841 gtggtgacag aggatgtgga aggtgacaag aaaatttata atgctgaagt tgctgaatgg
      901 gataagttac ggcaaagatt gcttgatgcc agaaataaaa tcacctctgc tgaatctgcg
      961 gtaaattc
//
LOCUS       DAZFZC010000356          966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFZC010000356 DAZFZC010000000
VERSION     DAZFZC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.367"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>966
                     /locus_tag="ACHKXP_005483"
     CDS             <1..>966
                     /locus_tag="ACHKXP_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7650233.1"
                     /translation="LDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAG
                     NGNVATVASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHH
                     VLKSAVGAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNI
                     RVHAQVSGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADAL
                     VRNALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFV
                     TANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGL"
BASE COUNT          198 a          258 c          319 g          191 t
ORIGIN      
        1 actggatgtg gaagaggaag agcactacga caacaccgca gcggaaatcc gtcaggtgga
       61 tgcgcacctg aagcgtctgc gtgaactgga aaccagtaag gccgccacgg cacagccggt
      121 gaaacaggcc ggtaacggga atgtggccac ggtggcttca gcgccggtga tccgtgttga
      181 gcagaaactg gagaagggga ttggtttcgc ccgctttgcc aaatcactgg ccgcggctaa
      241 aggtgtccgc tctgaagccc tggaagtggc ccgtcgtcag tatccggatg acagccgtct
      301 gcatcatgtc ctgaaatcgg cggtgggggc agggaccacc acggacccgc agtgggcagg
      361 cagcctgtct gaataccagg aatatgcgca ggactttatt gattacctgc gtccgcagac
      421 cattatcggg cgatttggtc agggcgggat ccctgcactt cgtcaggtgc cgttcaatat
      481 ccgcgtgcat gcccaggtgt ccggtggtgc tgccggctgg gtgggtgagg gtaaggccag
      541 acccctgacg aagtttgatt ttgaatccat caccttcagt catgcgaaag tgtcggccat
      601 tgcggtactg acggaagaat tgatccgttt ttccagtccg gctgctgatg cactggtccg
      661 taatgcgctg gcggaagcgg tggtggcgcg tctggacaca gactttgtgg acccgaaaaa
      721 agcggcggtg gcggatgtct ccccggcgtc catcacccat gatgtgaagg gcacggcatc
      781 aaccggtaac ccggatgcgg atgccgaggc cgcgtttggc cagtttgtga cggcaaatct
      841 gcagcccacc ggtgcggtct ggctgatgtc cagcaccaat gccctggcgc tgtccatgcg
      901 taaaaatgcg ctggggcaga aggaatatcc ggacatgacc ttgctgggcg ggaccttcca
      961 ggggct
//
LOCUS       DAZFZC010000357          952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFZC010000357 DAZFZC010000000
VERSION     DAZFZC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.368"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(405..584)
                     /locus_tag="ACHKXP_005484"
                     /pseudo
     CDS             complement(405..584)
                     /locus_tag="ACHKXP_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            723..>952
                     /locus_tag="ACHKXP_005485"
     CDS             723..>952
                     /locus_tag="ACHKXP_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7650234.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKEL"
BASE COUNT          274 a          197 c          205 g          276 t
ORIGIN      
        1 aatgtattgc aaaatggtag atgctgagta atcaacagac ttgtatgtaa agtcaagatt
       61 aacgttgcta aagcacgcat caattttctg ataaaaggat ggggttgttt ttttcgataa
      121 aaaaaattct tgtctaacca ctggtgaaat aagattatta ttaccccgaa ttaatactga
      181 gcctaacaat agaatacaaa aaaaacaaaa tgctttaaat aaggggagca ctataagtta
      241 tcctctttac tttatgctta ccaactcatt tatgttactt tataacataa cccatcttgc
      301 gttgtaaaca gactcgtttt agttccatgc accttttgag atcgtgacca aataatggcg
      361 ttagtgatgt tcttccagtg tcatatggtt aagtagttga tatggcgttc actgttatat
      421 tctgacaacc agtttgccgc cagttcccgc ttttcatcca gcgttctgaa cagacaaaaa
      481 ccgagtattt ttgttcggta tgtccggtta aaactctcga taaaagcgtt ctgcgccaac
      541 ttacctgatt gggtaatctc cagttttact acatgttttt ctgcgcatta cgtccgtgtt
      601 ggataaaaaa aatcctggcc ataatctatg cgaatcatag ctggatagac atggttttcc
      661 gcaatccttg aaccgccccg ggaatcctgg agactaaact ccctgagaaa gaggtaaaca
      721 ggatgactaa aaatactcgt ttttcccccg aagtccgtca gagggcgatt cgtatggttc
      781 tggaaagtca gggcgaatat gactcacagt gggcggcaat ttgttccatt gccccaaaga
      841 ttggctgtac accggagact ctgcgtgtct gggtacgcca gcatgagcgg gataccggag
      901 gcggtgatgg cgggctcacc accgctgaac gtcagcgtct gaaagagctg ga
//
LOCUS       DAZFZC010000358          951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFZC010000358 DAZFZC010000000
VERSION     DAZFZC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.369"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(176..847)
                     /gene="queE"
                     /locus_tag="ACHKXP_005486"
     CDS             complement(176..847)
                     /gene="queE"
                     /locus_tag="ACHKXP_005486"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO7650235.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          254 a          235 c          210 g          252 t
ORIGIN      
        1 actttaccgc aataattttc actccagcga aaaattaatg ccacagaatt tgtagagaat
       61 gctaatgatt agccctggat gggtaaaatg gaggagtttt cagaggcgac aaacaatata
      121 aatgagtaga agtctttaca ggttccaccc atttataacg cttataaatg tttaatcagg
      181 caatatttag atatttatgt gtttgcatcg acaaacgcca attacgcgca atgcaggttt
      241 caatgcacaa acgtgtggca tcatcctttt ggctaatcgg ctgcagtgca atgactcgcg
      301 gtttatcatc ggtcagcgtt gccagtagtt catccagtgc ttcaatatcg cgtacgcgcc
      361 ccactggatg cttgatttcg ttggctcgct ccagtgcctg tgacaacact tcatagccgc
      421 cgcgcatgtt cagctttggc gatacggtaa cccaggtatt cggtgtgcag cgtacctcat
      481 gagtaccact ggtttcgatc tggcagctaa aaccgttctt ttcgagcaga tcagtcagtg
      541 gcagcaaatc atgaatgcaa ggctcaccac ccgtaatcac cacatgccgc gcggtgtatc
      601 cctggcgacc aatgacagcc agcaaatctt cactgctcgc agccccccac ttatcactct
      661 ctttggtctt cgccagaatg ctgaaaaggg agacttcccg atcctcaagc ttttcccagg
      721 tgtgtttggt gtcgcaccag gcacagccaa ccgggcatcc ctgtaaacga ataaaaatgg
      781 cgggaacgcc ggtaaagtaa ccctcacctt gcagggtctg gaacatctcg ttaatcgggt
      841 actgcatagc attctctgtg aagtggataa ttgttaatta ttgcagatcc tgccacaaca
      901 atcatgtctt attaacattc tgttacaggc aggtttaaag aaaaatgcga a
//
LOCUS       DAZFZC010000359          930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFZC010000359 DAZFZC010000000
VERSION     DAZFZC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.370"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..841
                     /locus_tag="ACHKXP_005487"
     CDS             155..841
                     /locus_tag="ACHKXP_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO7650236.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPLH"
BASE COUNT          269 a          180 c          217 g          264 t
ORIGIN      
        1 ggaaggcaag ctgcaatggc aaaataggga tggttgtaat aatcaaaaca tgatactaac
       61 tatatattga tcgttgcatt gttggcatgt aacacgcttc agcgagtaac attggtaatg
      121 aaatataatc aacatggata ggtgaaaatt aattatgagc tttatggctt ttgtaacttg
      181 tttgacaatt gcatttatcg ccggttttgc tgttgccaga aaattgacac tccgaaaagt
      241 tgcctcatcg gtaatcatcg ctaaatcgca accagtccct tcatctgcaa cttcccctgc
      301 cgctaaggcg tcgacaagga gctatatacc gcagaaaacc tatcgtgact ctcgttggca
      361 agagcgagat gagttatttc agggaatggt tgccgaaggt attttattgg aaagaaaaga
      421 gtggttaatc acgcgttgtg aaaaaatgta tttcgataat ctggaaaaat ggtttggaca
      481 ctattgtcat atccattgcc aggtctctct ggggcgcttg atcaactttc cagaacaacc
      541 tgagtttagc gaagaagaaa gaaagcgttt ctttgcaatt tataatggca tggcaatgga
      601 ttatgttctg gtttcaaaga agaacaataa aatagtctgt gttattgagc tggatgaccc
      661 gagtcatgaa agggcggatc gcattattcg tgaccgttgg ttggatacga ttatgactct
      721 tgcacgggtg ccttttgtac gagcttctgt cactaaattg gaggaaatcc ctccggtctg
      781 ggagactcgt gacaaaattc gcccattagt cgcggcgaca aagagtgaac cgttgcatta
      841 actaagtatc ccggtcgggg cttctcatcc ccccggtgtg tgcaatacac gaaaaaaaag
      901 cccgtacttt cgtacgagct cttctttaaa
//
LOCUS       DAZFZC010000360          926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFZC010000360 DAZFZC010000000
VERSION     DAZFZC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.371"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            140..>926
                     /gene="escD"
                     /locus_tag="ACHKXP_005488"
     CDS             140..>926
                     /gene="escD"
                     /locus_tag="ACHKXP_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO7650237.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNT"
BASE COUNT          281 a          191 c          168 g          286 t
ORIGIN      
        1 aaactcacgt aatcattaat tatgaatatt ccattaaata gtactgtaca acagcttcct
       61 ttctactcta ttcttagttg atgtaagttc accatatttt ttctcattgt tcaaccacca
      121 ggatgaataa aatttaaaaa tgttatcctc atataaaata aaacttctca atggtgcgat
      181 gcgtaatcgg gaattacaac ttccgatggg aaacctaacg ataggcacag aagataacga
      241 tattgtttat tttccccttg aacaaggcct caatcagttt ttacttgata tacgtgagga
      301 aggtgttttc cttttatctc cggttgaatt ttggattgat ggacagccca ccccctatga
      361 agcagatcag tcattacctg tcggaaaggt catcgatata gcgggatgtt gcttcatcat
      421 tggtgatatt gaccacacct tgccattgtc tgatgtgcca gaaaggttct ctgccaaaaa
      481 tcgacgtaaa aagcgtctta tcctggctag cgtgattggt gctgcgattg cgttgtctgg
      541 cgccatcggc agttacgttt tgctttcccc aaaagctgaa cccccagcgt ttaccagagc
      601 tgatgtatac cagcagctaa aagaaaacaa acttcatgca atcactctgg tatggcatgg
      661 gaaaaatgtc gcgctttatg ggcgttgcga atcaaccact gatttaactc cattttttaa
      721 ttacctgaaa gaaaaaaata ttttttatta caacaatatc atttgcaata accaaatcat
      781 ctccgcgatt aatgatattt taacggaata cggttataag gatatcatca ttacaaaagg
      841 taataagcct ggattcttcc tgttatcggg atacattccc ccttcaccaa aatggagcga
      901 agttgaaaat ctgttactga atacgc
//
LOCUS       DAZFZC010000361          910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFZC010000361 DAZFZC010000000
VERSION     DAZFZC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.372"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(21..467)
                     /locus_tag="ACHKXP_005489"
     CDS             complement(21..467)
                     /locus_tag="ACHKXP_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO7650238.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(464..814)
                     /locus_tag="ACHKXP_005490"
     CDS             complement(464..814)
                     /locus_tag="ACHKXP_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7650239.1"
                     /translation="MAISAGRLTQMISVLKPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(825..>910)
                     /locus_tag="ACHKXP_005491"
     CDS             complement(825..>910)
                     /locus_tag="ACHKXP_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650240.1"
                     /translation="EREDLVQGELPFSVSVLIYDLRCPTVL"
BASE COUNT          192 a          322 c          207 g          189 t
ORIGIN      
        1 aggatacaaa tccgcctccg tcatcgtctc agtacctcat caatggcccg gttcatccga
       61 gcaatcgcca cctgtgccgc ctgttcactg cgcacatcaa atgccgggcg cacaaacggg
      121 tgcggtggca tattcacggt ccccatttcc acaaaccgcc agtagaaagc attgcgcggg
      181 ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg gattaacacc ccggatatgc
      241 acaccggatt ccatcccgcc atcgcgggag caccgggaaa ggaccaccac attgcggcga
      301 agttttcccc tgcgtaccgg tgcccgtgac accacttctt ctttcagcac attcgcaccc
      361 gcacgggttg cctcacgcag cacccggtta ttttccgcac cactcagaag ctgcaaatcg
      421 cggctgatgt cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcccctccca
      481 gccgacagag aatttccaga cgcccgccgg tcgcatccgg cacgggcagc ccgacaacgt
      541 tcaggatccg gtcacgccat ggaccactca gcacatgaag tcgtgacgct gccgtgattt
      601 cccggccgga ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccgg
      661 actgcatccg ctcccggctg ctcctgccac ggatatccgc atgaattttc ccgcatgaca
      721 cccattcttc cgtcatttct ccggcagcat tacgggttaa caccggcttc agaacactta
      781 tcatctgtgt cagacgacct gcagatattg ccattcctcc ctcctcataa caccgtcgga
      841 caacgcaaat cgtaaatcag cacggacaca gaaaacggca gttccccctg cacgaggtct
      901 tcccgctcag
//
LOCUS       DAZFZC010000362          904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFZC010000362 DAZFZC010000000
VERSION     DAZFZC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.373"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>904)
                     /locus_tag="ACHKXP_005492"
     CDS             complement(<1..>904)
                     /locus_tag="ACHKXP_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO7650241.1"
                     /translation="LEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHF
                     YHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPHHVYQPYRLPGQQHDEESGLY
                     YNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQIDPMGLLQTWDDARSGACTG
                     GVCGVLSRIIGPSKFDSTADAALDALKETQNRSLCNDMEYSGIVCKDTNGKYFASKAE
                     TDNLRKESYPLKRKCPTGIDRVAAYHTHGADSHGDYVDEFFSSSDKNLVRSKDNNLEA
                     FYLATPDGRFEALNNKGEYIFIRNS"
BASE COUNT          225 a          246 c          166 g          267 t
ORIGIN      
        1 cactatttct gataaaaata tattctcctt tattattaag cgcctcaaat cgtccatcag
       61 gtgttgcgag ataaaatgct tcaagattat tatctttact tcttacaaga tttttatcgc
      121 tacttgaaaa aaattcatca acataatcgc catgactatc tgcaccgtga gtatgataag
      181 cagcaactct atctatacct gtgggacatt ttcttttcag aggatatgac tcctttctta
      241 aattatcagt ttctgcctta gatgcaaaat attttccatt agtatcttta cagacaatac
      301 cagagtattc catatcatta catagagatc tattctgcgt ttctttcaaa gcatctaacg
      361 cagcatctgc agtactatca aatttacttg gtcctattat acgtgaaaga acaccacaaa
      421 ctccccccgt acatgctcca gatctggcat catcccaagt ctgcaataat cccatagggt
      481 caatttgttg tagtggattt aaaggatact gatataaatt ccatccccct ttcaacccca
      541 tcgggtcctg agtaatatac cgcccctgca acggatcgta gtaccggtga cggttatagt
      601 acagccctga ttcctcatca tgctgctgcc ctggcagacg gtacggctga tacacatgat
      661 gcgggttctc ctcattaagc tggttgcccc attcatcata ctccccgcgc cacgccgtat
      721 tgccgtcttc gctgatgagc gccagcggca ggccccggtg gtcgcagtgg taaaaatgaa
      781 cttttcgcgc cggtgtgtat tccggctcca cctgtctggc cagttgctcc accgtcagcc
      841 cgcactgcgc aagccacgcc cggctttcac tgctcacgcg gtctgcccgg atttcttcct
      901 ccag
//
LOCUS       DAZFZC010000363          904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFZC010000363 DAZFZC010000000
VERSION     DAZFZC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.374"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..794
                     /gene="yeeO"
                     /locus_tag="ACHKXP_005493"
     CDS             <1..794
                     /gene="yeeO"
                     /locus_tag="ACHKXP_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO7650242.1"
                     /translation="SRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT          185 a          180 c          256 g          283 t
ORIGIN      
        1 tttctcgtta tattggcgca gttgcaattt tgtgggtgct ggcgattggt tttaatcctg
       61 cgctaaggat ttcgttaaag agctatttta aaccgctgaa ttttagcatt atctgggaag
      121 tcatggggat tggtattccc gcgagtgtcg aatcagtgtt atttaccagt ggtcggttat
      181 taacccaaat gttcgttgcc gggatgggga ccagtgttat tgccggaaat tttatcgcgt
      241 tttcaattgc ggctcttatc aacttacccg gaagtgcgct cggctctgct tctacgatca
      301 ttacaggccg aaggttgggg gtagggcaga tagcgcaagc agagattcag ttgcggcatg
      361 tgttctggct ttccactctt ggattaacgg ccatcgcctg gctaacggct ccctttgccg
      421 gggttatggc atcgttttac acccaggatc cacaggttaa acatgtcgtt gtgattctga
      481 tttggctaaa tgctttattt atgcctattt ggtccgcctc atgggtgcta cccgctggat
      541 ttaaaggtgc tcgtgatgcc cgttacgcca tgtgggtttc gatgttgagc atgtggggtt
      601 gtcgggttgt agtcggttat gtgctgggaa tcatgcttgg ctggggtgtg gttggtgtct
      661 ggatgggaat gtttgccgac tgggctgtgc gggccgtgct gttttactgg cgaatggtta
      721 ctggacgttg gctatggaaa taccctcgac ccgagccgca aaagtgtgaa aaaaagccag
      781 ttgtgtcgga ataaacgaca aaatgcagat tatttcagca aacgatttca aatttaaaaa
      841 acaggctttg acattgtggg tgggcatcgc taatattcgc ctcgttctca cgattcctct
      901 gtag
//
LOCUS       DAZFZC010000364          858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFZC010000364 DAZFZC010000000
VERSION     DAZFZC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.375"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..396
                     /locus_tag="ACHKXP_005494"
     CDS             <1..396
                     /locus_tag="ACHKXP_005494"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7650243.1"
                     /translation="LTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGINDNLL
                     FKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLNISC
                     EVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            393..740
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005495"
     CDS             393..740
                     /gene="tnpB"
                     /locus_tag="ACHKXP_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7650244.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          213 a          239 c          218 g          188 t
ORIGIN      
        1 ttaaccgcag tgcgtaaaaa aagccctaat tatcccgttg agttcaaaat caaaatggtt
       61 gaactctcgc atcgaccaga gatctccgta gcgcaactcg ctcgtgagca tgggatcaac
      121 gataatttgc tgttcaagtg gcgccagtac tggcgcgaag gaaaactacg tcctccttca
      181 acaacagaaa acaacgtgcc tgagctgctc ccgataacac ttgatgccga agatgttgtc
      241 cctacaacct ccccccggtc acaacctgta gctgctgcga cacctgaatc actcaatatc
      301 agctgtgaag tgacgttccg gcacggatca ctccgtctga atggtgccat cagcgaaaat
      361 atcctgaacc tgctgatacg ggagctcaaa cgttgatccc attaccatca gggacaaaga
      421 tctggctggt cgctggcatc accgatatga gaaacggctt caacggcctg gcggcaaagg
      481 tgcagacgac gctgaaagac gatccgatgt caggtcacgt ttttatcttc cgtgggcgta
      541 atggcagtca ggtaaagctc ctctggtcta ccggcgatgg actgtgtctg ctgaccaaac
      601 ggctggagcg cggccgcttc gcctggccgt cagcccggga tggcaaagtg ttcctcacac
      661 cggcacagct ggcgatgctg ctggaaggta tcgactggcg gcagcctaaa agactgctta
      721 cgtccctgac tatgttgtaa gcctctttat cctggtcgac gctgaatgag cctggtaata
      781 tacccggtat gagcagctca cttcctgacg atatcaatgc actgaaacgt ctccttgccg
      841 aacaggaggc gctgaacc
//
LOCUS       DAZFZC010000365          853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFZC010000365 DAZFZC010000000
VERSION     DAZFZC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.380"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(20..175)
                     /gene="hokD"
                     /locus_tag="ACHKXP_005496"
     CDS             complement(20..175)
                     /gene="hokD"
                     /locus_tag="ACHKXP_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7650245.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          229 a          214 c          182 g          228 t
ORIGIN      
        1 ttctcccccg cctggtctct tactcctcag gttcgtaagc tgtgaagaca gcgacctccg
       61 tctggccggt tcggattcgt acctcgcaga ggtctttcct cgttaccagt gccgtcacta
      121 tgacggttaa acagatgacg atcagggcga ttaacatcgc cttttgctgc ttcatagcct
      181 gcttctcctt gcctttcggc acgtaagagg ctaacctaga tttgccgttc atagattgag
      241 cctcagatta atgttaagcg tcttgcagga cgcgtaatgt taactggggc ttttctctat
      301 ctgcctttgg tgttcatgcc tgagacagat agcctcaagc acccgcagcc attctactta
      361 actcccgtta cctcgccaat atgaaatcag tcagaaaggc gatccataag aacaacagca
      421 aggcaataaa ttgccattac agcagcaata gccagcgcac atttgagaac cagcaccaca
      481 acctcctgta ttggacgtac accagtcctg ataaatatga ggctgtctcg tcagtgattc
      541 aatacaacta ttgggtatag tttctgtgat tttgttctgt agaaatggaa cataataacc
      601 tgtcaccacc agcacttctt taaatacgcc aagtccgacg caagctaacc ttctagcccg
      661 ctttgagcga agaacggact gagtaattca ggccgttagc gacactatga catcattagc
      721 aaccaactag gagttggtaa tagatgagtt aattaattga ttaaaaaccg acgattttac
      781 tagattacgg gaagaagaaa ggccgctggt acggcctccc agagactagt ctattgttat
      841 gcttagaaac gct
//
LOCUS       DAZFZC010000366          850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFZC010000366 DAZFZC010000000
VERSION     DAZFZC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.381"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            241..316
                     /locus_tag="ACHKXP_005497"
     tRNA            241..316
                     /locus_tag="ACHKXP_005497"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Ile,seq:cat)
BASE COUNT          254 a          161 c          186 g          249 t
ORIGIN      
        1 ttattagata caaaatcatt acaaagtatc attgaatcga tgataactat agatagtgct
       61 aattattaac atttgcttgt tgactgcgag tatcgacttt gcgaatggag gcggtgtcgg
      121 gttttatgtc gcgtgtaatc acgacccaag gtgaatggga acgcataaaa atctgttatt
      181 tacatcatat gatcatcatg tttattgcgt ggtttataag tattgttatt gcacactcaa
      241 ggccctttag ctcagtggtt agagcaggcg actcataatc gcttggtcgc tggttcaagt
      301 ccagcaaggg ccaccaaatc attatttacg tatcaggaag agttaagggt acaaggggta
      361 ctcgatggcg gtctatattt catgactaac gccatttcaa ggaagaagtg cggtgggtgg
      421 tttcgaatga gggctggtgt tttggtacgt ggcacgccac tgcaatgtat ttatccatca
      481 gtactcattc aaaagtacct ggaaaaaatg cctgctatgc caagcgggca aattgcttac
      541 atgtggaatt aagtaattaa cgataaagcc agagtattat catttattat accccttcat
      601 ttaatcaggt taattactca aatcactgaa ctattctctg ttcgctcact gatgataagt
      661 gagtaccaca accaatgtat gtagaacaat gccgtagagt aaggatgaaa tgtctctgcc
      721 acgccttaaa catctactac tgaccagaac acccgccgca ggcagatgag ttttgtgagc
      781 gttacaggag cgtggctatg ctcattaacc aacgctattg agatataaat ttctaacact
      841 tgaaaaacga
//
LOCUS       DAZFZC010000367          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFZC010000367 DAZFZC010000000
VERSION     DAZFZC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.382"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..104
                     /locus_tag="ACHKXP_005498"
     CDS             9..104
                     /locus_tag="ACHKXP_005498"
                     /inference="COORDINATES: protein motif:HMM:NF026967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2 fold immunity protein"
                     /protein_id="HFO7650246.1"
                     /translation="MEGKQPKTLGGNFTILIAKKDGQVLHVIHTK"
     gene            655..>842
                     /locus_tag="ACHKXP_005499"
     CDS             655..>842
                     /locus_tag="ACHKXP_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6258 family protein"
                     /protein_id="HFO7650247.1"
                     /translation="MFDRIYLGDRAIKKIEFDLWSKQIRIQVNLISRIAYGTTEWIFY
                     DNEDLEDGYLVFLDVSFF"
BASE COUNT          275 a          137 c          180 g          250 t
ORIGIN      
        1 gttggaaaat cgagggaaaa caaccgaaga cgttaggagg aaattttaca atactaatag
       61 cgaaaaaaga tgggcaagtg ttgcatgtaa tacacacaaa ataatccatt gtacagtcga
      121 ttcaaatatt ttaccaaggt gtgtttcgta actattttgt gtacaatatg actctacata
      181 tcgacgaaaa aaccacttga tttgacagga acacattcaa aaaatttctt ttttcatttc
      241 aggagcgatt ttcatctgtt acttgaagta tctgtgagat taagtgactc cccctaacgt
      301 cgtactaaat agtaattact ggccataccg ggagcagtat tcggcgcttc ccacatgcag
      361 ctgaataacc gcatcagcga agatgtattg tatttttact atcacaatgt gtacggacgg
      421 tttatggaaa agaatgaaca ttagatacgc gatgcggcgg caggttacac ggttatcact
      481 acgatagcca atactggctg gtgcattacc atcgtgaaca ataggacgcc gtactgctgg
      541 aaatttctta cctttatgac tccccgcgcc gccgtatcgg aaaacgggaa gcaatcaaga
      601 tgaggctgat ataatgtgcg ggatgcctga gtatttttag aatagggggg taatatgttt
      661 gatagaatat atcttggtga tcgagcaata aaaaaaattg aatttgattt atggagtaaa
      721 caaattagaa ttcaggttaa tttaatctca aggatagcct atggtacaac agaatggatt
      781 ttttatgata atgaagactt agaagatggt tatttggtat ttttagatgt tagtttcttc
      841 aa
//
LOCUS       DAZFZC010000368          823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFZC010000368 DAZFZC010000000
VERSION     DAZFZC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.383"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..782)
                     /locus_tag="ACHKXP_005500"
     CDS             complement(<1..782)
                     /locus_tag="ACHKXP_005500"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO7650248.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIIL"
BASE COUNT          180 a          200 c          184 g          259 t
ORIGIN      
        1 aggatgatcg tataacgtga ttttcggtct acaagtgtgg ctatatgaga gttttttgta
       61 cctgagacta aatcgccctc ccaatgcccc agagagcgtc tgttatcgat atttcgggaa
      121 cgttcgtgaa ttggtgttcc gttcactatg ttaatcgtac ctctttcgcc tttgcgggta
      181 tgacgcctgc catggcgaag gctatgcgac cgtcgcagat gctgtatatt caggtggtgt
      241 agcgcttcac ggctacgaaa gtacagcgtt ttataaattg tctcaggtga tattcgcagc
      301 gttttttgac gtggttttgt tcgccttaac catcctgata tttgctctgg agaccatttc
      361 atctccagct tttccagaac aagctttcgc aatggtaaat tttgatccag taagcacggt
      421 tttggccttt tcgccattct gttggctcgg ttattagcat caacagcttt gtaatagcgt
      481 ctgccccgat tacgctgaac ttcacgtgag atcgtcgaag gactgcgatt cagcgcagta
      541 gctatcgcac gaatgctcat tttggctgac aaaccagctc gtatctcctc gcgctcagac
      601 agtgtcaggt gagctacagc ccgcttacgc tcatggggtt ttatgccgcc agtatccctt
      661 aacatagtga agatcgttcc gggttttgaa cccaggatat tcgctatttc actgaagcct
      721 gttccgttct tccatagttc aaaaacagag gctttttcct ctgctgtaaa tgttcgtctc
      781 attcaaaaaa cctccgcaac cccatgtttt cacataactg ttg
//
LOCUS       DAZFZC010000369          800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFZC010000369 DAZFZC010000000
VERSION     DAZFZC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.384"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..800
                     /locus_tag="ACHKXP_005501"
                     /pseudo
     CDS             1..800
                     /locus_tag="ACHKXP_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          189 a          196 c          249 g          166 t
ORIGIN      
        1 acttgctgcc gccatgaagc agattaaaga actccagcgc ctgctcggca agaaaacgat
       61 ggaaaatgaa ctcctcaaag aagccgttga atatggacgg gcaaaaaagt ggatagcgca
      121 cgcgccctta ttgcccgggg atggggagta agcttagtca gccgttgtct ccgggtgtcg
      181 cgtgcgcagt tgcacgtcat tctcagacga accgatgact ggatggatag ccgccgcagt
      241 cgtcacactg atgatacgga tgtgcttctc cgtatacacc atgttatcgg agagctgccc
      301 acgtatggtt atcgtcgggt atgggcgctg cttcgcagac aggcagaact tgatggtatg
      361 cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct gttgcttgag
      421 cgaaaacctg ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc cgtgaaagaa
      481 agcaatcagc gatggtgctc tgacgggttc gagttctgct gtgataacgg agagagactg
      541 cgtgtcacgt tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc ggtgactacc
      601 ggcggtttca acagtgaaac agtacaggac gtcatgctgg gagcggtgga acgccgcttc
      661 ggcaacgatc ttccgtcgtc tccagtggag tggctgacgg ataatggttc atgctaccgg
      721 gctaatgaaa cacgccagtt cgcccggatg ttgggacttg aaccgaagaa cacggcggtg
      781 cggagtccgg agagtaacgg
//
LOCUS       DAZFZC010000370          795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFZC010000370 DAZFZC010000000
VERSION     DAZFZC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.385"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..296
                     /gene="ler"
                     /locus_tag="ACHKXP_005502"
     CDS             <1..296
                     /gene="ler"
                     /locus_tag="ACHKXP_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO7650249.1"
                     /translation="AKRLREQEIQSVIESIQKQITYYNITLQELGYTNVPDDGLARRN
                     SSKGVYYRNEEGQTWSGVGRQPRWLKEALLNGMKKEDFLVKDTEDEIIPLKNI"
     gene            311..529
                     /gene="escE"
                     /locus_tag="ACHKXP_005503"
     CDS             311..529
                     /gene="escE"
                     /locus_tag="ACHKXP_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO7650250.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRIIEIMLIRINNK"
     gene            542..>795
                     /locus_tag="ACHKXP_005504"
     CDS             542..>795
                     /locus_tag="ACHKXP_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system filament chaperone"
                     /protein_id="HFO7650251.1"
                     /translation="MSQTKNRELLDKKIRSEIEVIKKIIAEFDVVKENVNALSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETMSPP"
BASE COUNT          336 a          120 c          165 g          174 t
ORIGIN      
        1 aagcaaagcg actgcgagag caggaaattc aaagtgtaat tgagtcgatt caaaagcaga
       61 ttacttatta caatataacc ctacaagagc tgggatatac taatgtgcct gatgatggcc
      121 ttgctcgccg gaactcatcg aaaggagttt attatcgcaa tgaagaaggg cagacctggt
      181 cgggagttgg ccgacagcca cgctggctta aagaagcact gttgaatgga atgaagaaag
      241 aagattttct tgtgaaggac accgaagacg aaataatacc gctgaaaaat atttaacatg
      301 aaatcattaa atgataacga taactgaact ggaagacgaa ataataaaaa ataaagaagc
      361 cgcaaatatt ttcattgaaa aaataaacga caaaaagaac gaaatccatg aaaaaatgaa
      421 acaccccctg gataaagtta cctacaatga agcaaaggaa ctgctcattg cctgcgatgc
      481 ggcaatcagg atcatagaga taatgctaat cagaattaat aataaataga ggacgagtat
      541 tgtgagccaa acaaaaaata gagaactgtt agataaaaaa ataagatcag aaattgaggt
      601 gataaaaaaa ataatcgccg aatttgatgt cgtaaaagaa aacgttaatg ctttaagtga
      661 aaaagcaaaa acagatcctc aggctgcaga aaaattaaac aaattaattg aggggtatac
      721 atatggtgaa gagcgaaaat tatatgattc tgccttaagt aagattgaaa aactaataga
      781 gacaatgagt cctcc
//
LOCUS       DAZFZC010000371          789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFZC010000371 DAZFZC010000000
VERSION     DAZFZC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.386"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..765
                     /locus_tag="ACHKXP_005505"
     CDS             49..765
                     /locus_tag="ACHKXP_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7650252.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          261 a          136 c          169 g          223 t
ORIGIN      
        1 ttaacgattt accagtaatg taaataaatt ttcgaggaga tcattccagt gggacgtaaa
       61 tgggccaata ttgttgctaa aaaaacggct aaagacggtg caacgtctaa aatttatgca
      121 aaattcggtg tagaaatcta tgctgctgct aaacaaggtg aacccgatcc agaattaaac
      181 acatctttaa aattcgttat tgaacgtgca aagcaggcac aagttccaaa gcacgttatt
      241 gataaagcaa ttgataaagc caaaggcggc ggagatgaaa cgttcgtgca gggacgttat
      301 gaaggctttg gtcctaatgg ctcaatgatt atcgccgaga cattgacttc aaatgttaac
      361 cgtacgattg ctaacgttcg cacaattttc aataaaaaag gcggcaatat cggagcggca
      421 ggttctgtca gctatatgtt tgacaatacg ggtgtgattg tatttaaagg gacagaccct
      481 gaccatattt ttgaaatttt acttgaagct gaagttgatg ttcgtgatgt gactgaagaa
      541 gaaggtaaca ttgttattta tactgaacct actgaccttc ataaaggaat cgcggctcta
      601 aaagcagctg gaatcactga gttctcaaca acagaattag aaatgattgc tcaatctgaa
      661 gttgagcttt ccccagaaga tttagaaatc tttgaagggc ttgttgatgc ccttgaagat
      721 gacgacgatg tacaaaaagt ttatcataac gtcgcaaatc tctaattatc ttttaaagaa
      781 atctgtctt
//
LOCUS       DAZFZC010000372          786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFZC010000372 DAZFZC010000000
VERSION     DAZFZC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.387"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..608
                     /locus_tag="ACHKXP_005506"
     CDS             <1..608
                     /locus_tag="ACHKXP_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO7650253.1"
                     /translation="SPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQSN
                     FMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVIGK
                     ETKSHIFKAVESTIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFNTQ
                     TRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          255 a          141 c          174 g          216 t
ORIGIN      
        1 ttagtccaat gacaatggta ggtagatcgt tagctcaggc ggctacacaa actcttagac
       61 ctgcggtcac cagagctgcg acacaagcgg gggtgggagc cagtggtatg aagttcgtgc
      121 ccatgcagtc gaactttatg attaatcatg gcaaactgac taaccagcta cttcaggccg
      181 tggccaaaca aacccgtagc ggtgatactc agcaatggtt caaacaggag caaattactt
      241 atatatccag aacagtaaac agagctattg atgactattg catgagtaat aattcggtaa
      301 ttggtaagga gaccaaaagc cacattttca aggctgttga aagcacgata aagcaacctc
      361 tcgatatgaa ttgtgcacaa acgtcgattg gccatttctt acagtccaac aaatacttta
      421 ataaaaaagt tgatgagcag tgtggcaaag gggtggatcc aattacacgc tttaacaccc
      481 agaccaggat gatagaacaa gtctcacaag aaatctttga acagaaattt ggtggtttga
      541 aagttagtga tatcaaagct ctgactcaga gcacgattgc acagcatgta caggatactc
      601 ggttgtagga ataacctgtt tatatgcgtg ttgggcataa gttaagcttc ctggtgtata
      661 gcgtagtctt tgctgatgta agaaaaggct atatacggaa acttgatata ctataaactt
      721 cattatttat tagattgaaa aaaagttatt taattgattt gttttgtaat tttaatgctt
      781 caattg
//
LOCUS       DAZFZC010000373          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFZC010000373 DAZFZC010000000
VERSION     DAZFZC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.388"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(32..736)
                     /locus_tag="ACHKXP_005507"
     CDS             complement(32..736)
                     /locus_tag="ACHKXP_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO7650254.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          208 c          200 g          192 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DAZFZC010000374          761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFZC010000374 DAZFZC010000000
VERSION     DAZFZC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.389"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..211
                     /locus_tag="ACHKXP_005508"
     CDS             <1..211
                     /locus_tag="ACHKXP_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFO7650255.1"
                     /translation="LGLLNLHKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQ
                     PVQDPKPDLLSTMEHENVRGSEYYH"
     gene            226..>761
                     /gene="istB"
                     /locus_tag="ACHKXP_005509"
     CDS             226..>761
                     /gene="istB"
                     /locus_tag="ACHKXP_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HFO7650256.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQR"
BASE COUNT          232 a          179 c          183 g          167 t
ORIGIN      
        1 ccttggactc ctgaaccttc ataaaaaata cagtaaagcc agacttaatg cagcatgtgc
       61 aagagctctg aaaacaaagg tatggcgtct gtcaggtatt aaatcgatcc tggaaaaagg
      121 tctggataaa caacctgttc aggatccaaa accagatctg ttatccacga tggaacacga
      181 aaacgtacgc ggcagtgagt attaccactg atacgggatc caatgatgaa tcatctttac
      241 gaacaactga ccgcacttaa actcaccggc ttccgtgatg cgcttaaaaa gcaacttgct
      301 cagccgggca cataccagga gctgggcttc gaagaacgcc tgtcattact gacagcagaa
      361 gaactaacct gccgtgaaaa caggaaggca gagcgtctga tcaaacatgc acggttcaga
      421 cttaatgctg agttatcaaa gctggattat cgtaacaata gagggctgga cagggccctc
      481 atccgttcac tcagtcaggg aaactggtta accctgaaac aaaatatttt actgaccggg
      541 gccaccggca gcggtaaaac gttcctggca tgtgcacttg gtcataatgc ctgccgacag
      601 ggatacaagg tctactatta tcgccttaaa gcgctgatgg aacagtgcta tcaggggcat
      661 gctgatggaa gatacagcaa acttttgacc aggctgaata atagcgatct gctgcttctg
      721 gatgactggg ggctggaacc tctctcatca gaacagcgta g
//
LOCUS       DAZFZC010000375          751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFZC010000375 DAZFZC010000000
VERSION     DAZFZC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.390"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..226)
                     /locus_tag="ACHKXP_005510"
     CDS             complement(<1..226)
                     /locus_tag="ACHKXP_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7650257.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTAGVV
                     EQNLSRQALAAQKAGISVGQYKAAMRTLPAQ"
     gene            complement(207..620)
                     /locus_tag="ACHKXP_005511"
     CDS             complement(207..620)
                     /locus_tag="ACHKXP_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7650258.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFLDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVADVSEDDVALMMASAGISGGVRYVPAGW"
     gene            complement(647..>751)
                     /locus_tag="ACHKXP_005512"
     CDS             complement(647..>751)
                     /locus_tag="ACHKXP_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7650259.1"
                     /translation="MSGAVHADTDSTEVAKNNALTDDDFSAGKSSTAS"
BASE COUNT          178 a          241 c          175 g          157 t
ORIGIN      
        1 actgtgcggg cagggttcgc atggccgctt tatactgccc gacggaaatc ccggcttttt
       61 gtgcagccag cgcctgacga ctcaggttct gctcaacaac accggcggtt tttctggcgt
      121 cagtctccag tccggaaaaa tgacgcctta cccggctcat ctgctcatcg aaacggaccg
      181 catccagact caggtcaata acaagatcac caaccggctg ggacatatct cacacctccg
      241 gaaatccccg ctgaagccat cattaatgcg acatcatcct cgctgacatc cgccacatcc
      301 gcagacgata aaatctcacg ccctccgtcc ccaccaaacc ggacgcctcc ggcaagtcct
      361 gccgctttct gcatcagcat tttttcctca tccggcatct ccgtctgcgc ttcctcacgc
      421 cggggggcaa gcagactgaa atcagaggga tgcatatccg gatcgcaaaa aaacaggctg
      481 agtacggcgt acgtcagccc ggaaaaatgc atatccagtt gggtatcgag aaaataatgc
      541 gtacggtaaa aatgtcgcca gtcggcatat tcggtggatg tcatcccggc aagcatggcg
      601 cgccagtcag gcctccccat ctcacgcgcc agtctgaggg caaaattcag ctcgccgtcg
      661 aagactttcc cgcagaaaaa tcatcatcag tcagcgcgtt atttttcgcc acttcggtgc
      721 tgtcagtatc cgcatgaaca gccccgctca t
//
LOCUS       DAZFZC010000376          750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFZC010000376 DAZFZC010000000
VERSION     DAZFZC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.391"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>750
                     /locus_tag="ACHKXP_005513"
     CDS             <1..>750
                     /locus_tag="ACHKXP_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO7650260.1"
                     /translation="SMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTT
                     KGVFEDEGTNQSATDTLSSSVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFT
                     DKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNSTYSGSQQNTGASDTTAQPEP
                     VKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQN
                     QHESERNYQKAEQNSPNALQNEPETKQPEPV"
BASE COUNT          246 a          190 c          176 g          138 t
ORIGIN      
        1 catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt tttggccttg
       61 tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa tacattactg
      121 aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc gcaactgata
      181 ctctctcgtc gtcagtacca gaaactgatg cagtggaaac ggcaattccg gacaacgaaa
      241 aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg tgaggggccg ttctacttcc
      301 tcttcaccga caaggatggc gaaaaatacg gtcgcgcaaa caaactttct ggtctggata
      361 aggcactggc tgccggggct actgaaatca cgaaagaaga atatttcgcc cgcaaaaaca
      421 gtacatactc aggttcacaa caaaatactg gtgcatctga cacgaccgca caaccagagc
      481 cggtaaaagt taccgctgac gaagtaaaca aaattatgca ggcagccaat atcagccagc
      541 ctgacgccga taagttgctt gctgcctctc gcggagaatt tgttgcaggg attagcgacc
      601 cgaatgatcc gaaatgggtt aaggggatcc agacccgcga ttctgtaaac cagaaccagc
      661 atgaatcgga acggaactac caaaaagcgg aacaaaacag cccaaatgcg ttacaaaacg
      721 agccagaaac gaaacagcct gaaccagtgg
//
LOCUS       DAZFZC010000377          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFZC010000377 DAZFZC010000000
VERSION     DAZFZC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.392"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..105)
                     /locus_tag="ACHKXP_005514"
     CDS             complement(<1..105)
                     /locus_tag="ACHKXP_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="responsible for the influx of magnesium ions;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HFO7650261.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPD"
     gene            complement(257..313)
                     /gene="ysgD"
                     /locus_tag="ACHKXP_005515"
     CDS             complement(257..313)
                     /gene="ysgD"
                     /locus_tag="ACHKXP_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO7650262.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            475..>741
                     /locus_tag="ACHKXP_005516"
     CDS             475..>741
                     /locus_tag="ACHKXP_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650263.1"
                     /translation="MAHQLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAM"
BASE COUNT          203 a          177 c          184 g          177 t
ORIGIN      
        1 gtccggttcg acaagatcaa tccatactgc atttacaagg ggttgtgact cttcgacttc
       61 cagccgggtc agtcggttat tttccagttg aaatgcgctc agcatgaccg ggactcccaa
      121 tgcttaaaat atcggacagt tcggtgggca cacagaaaca aattgggttt cagaccatta
      181 aacagcctga ctcagcgcga cgggaaaaat tgaggtcgct gacaaccgct aaggctatca
      241 gcaaaaaggg atagccttag gagttgatcc tggatgacag gatagtgagc cagtatctac
      301 tgggtgtgtc caaggcgaat gtcctcttag agtgatcgtg cgcgcatgtt acgccagcaa
      361 aattttgccg tcaacacgca acagaacacc acagaacaat agttgaactt cgcgcttttt
      421 ggtactcgtc aaggtcgcta acaggataaa aaaatcccac atccaggacc tataatggcg
      481 catcagctac tcattggtaa aggaatgatc accttgaatc tcaaacggat ttttctcgcc
      541 ctcaccttac ttcccttgtt tgctgttgcc gctgatgatt gcgcactctc agatccgacg
      601 ctgaccgtac aggcgtatac cgttaatcct caaacagagc gggtgaaaat gtactggcaa
      661 aaagccaatg gcgaagcgtg gggaacgtta catgctctgc tggcggatat taatagtcag
      721 ggtcaggtgc agatggcgat g
//
LOCUS       DAZFZC010000378          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFZC010000378 DAZFZC010000000
VERSION     DAZFZC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.393"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>736
                     /locus_tag="ACHKXP_005517"
     CDS             <1..>736
                     /locus_tag="ACHKXP_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO7650264.1"
                     /translation="TPVNVLEPGQGKTRTGRLWVYVRDDRNAGSTMPAAVWFSYSPDR
                     KGIHPQQHLADYRGILQADAYAGYNALYESGQVTEAACMAHARRKIHDVHVRHPTTVT
                     GEALRRIGELYAIEAEIRGSPAEERLAVRKARTVPLMQSLYEWLQGQMSTLSRHSDTA
                     KAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRVVALGRRNYMFFGSDGGGESAAVM
                     YSLIGSCKLNGIEPETWLRHVISVINT"
BASE COUNT          178 a          195 c          224 g          139 t
ORIGIN      
        1 acacgccggt aaatgtactg gagccgggtc agggtaaaac ccgtaccgga cggctgtggg
       61 tctatgttcg tgacgatcgc aacgccggtt cgaccatgcc ggcagcggtg tggttctcat
      121 actctcccga ccgcaaaggc atccacccac agcaacatct ggcggactac agaggtatcc
      181 tgcaggccga tgcatatgcg ggttacaatg ctctttacga aagcggtcag gtaaccgaag
      241 cggcttgtat ggcacatgcc cgacgcaaga tccacgatgt acatgtccgc catccaacga
      301 cagtaacggg agaagcgctc cgtcgtatcg gggaactgta cgctatcgag gctgagatcc
      361 gcggcagtcc ggcagaagag cgactggcgg tcagaaaagc cagaacggta ccgctaatgc
      421 agtcgttgta tgagtggctc caggggcaga tgagcacgct gtcgcgccac tcggatacag
      481 cgaaagcgtt cacctatctg ctgaagcaat gggacgctct gaacgaatac tgccgcaatg
      541 gctgggtgga gatcgacaat aacctgtgtg aaaacgccct ccgggtagtt gcactggggc
      601 ggcgtaacta catgttcttc ggctctgatg gtggaggcga gagtgcggca gtgatgtaca
      661 gcctgatcgg tagctgcaaa cttaacggaa tcgagccgga aacgtggcta cgccacgtga
      721 tcagtgtaat caacac
//
LOCUS       DAZFZC010000379          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFZC010000379 DAZFZC010000000
VERSION     DAZFZC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.394"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..615
                     /locus_tag="ACHKXP_005518"
     CDS             <1..615
                     /locus_tag="ACHKXP_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7650265.1"
                     /translation="AGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGA
                     KTCEELAGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVT
                     ESDGKAFPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRAN
                     LALRRAKSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            631..>727
                     /locus_tag="ACHKXP_005519"
     CDS             631..>727
                     /locus_tag="ACHKXP_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7650266.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQL"
BASE COUNT          192 a          159 c          232 g          144 t
ORIGIN      
        1 gctggcgaag gtgaatcccg ggtaaccgaa gagcggccag taaaatctga agcacaggat
       61 atgctgaccg gggaggtcga acaaaaagtt accgcagaca tgatgattga gtttatcggt
      121 caggatgggg ctaaaacgtg tgaggaactg gcgggtaagt tcggtgtcag tactcgcaag
      181 gttgcttcca cgctggcggt ggtaaccgca acggggcggc tggcacgcgt taatcagaac
      241 ggtaaatttc gttactgcat gccgggcgat aatttaccag cagagccgaa agccgcgctg
      301 gtaacggaaa gtgatggtaa ggcctttcct cagccagcag gtgctgcgtt accagtccgg
      361 gaagccgcaa cacaggaaga aattaaaaca gaaactgtgg cggacattgt gcagccgttg
      421 ccatcgttta ccgaaacgca agcagatgag ctgatttttc cgtcccttcg cagggcaaac
      481 ctggcgctgc gcagggcgaa aagtgatgtt cagaagtggg agcgagtctg cgccgcgctg
      541 cgggagctga acaagcaccg ggatattgtt cgacagatta ctgattcttc ccgccgtgtt
      601 gtatcggaaa agtgattgcc ggaggcgctt atggcaaaag tatttacaca agaagagcgg
      661 gaaaaaatta aagggcaggt tgttgaacta gtacgccgga gtgggcgcga gacgttacgg
      721 caactgg
//
LOCUS       DAZFZC010000380          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFZC010000380 DAZFZC010000000
VERSION     DAZFZC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.395"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>725)
                     /locus_tag="ACHKXP_005520"
     CDS             complement(<1..>725)
                     /locus_tag="ACHKXP_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO7650267.1"
                     /translation="RTDGEGHRWQYRYDKDTLQLTEVINPQGESYLYVLDNCGRVTEE
                     HDWGGVVWRYRYDADGLCTARVNGLEETILYSRDAAGRLAEIITPEGKTQYAYDKSGR
                     LTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPD
                     ESLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVFRHSNRGFACEQGWNAASQL
                     VTQRAGFFPEETTWGGLLPSLVR"
BASE COUNT          122 a          223 c          210 g          170 t
ORIGIN      
        1 tcccgtacca gtgaggggag cagcccgccc catgtggttt cctccgggaa aaatccggcg
       61 cgctgggtga caagctggct ggctgcattc cagccctgct cacaggcaaa accccggtta
      121 ctgtgccgga acacttcacg tcccgcctca tcacggctga acgtcagcgt ctcatccccc
      181 ggcagaatga cctctgtcag ttctccggcg gcgttgtagc ggtacgtggt tttcagcagg
      241 gattcatcgg ggtgtctgtc gcgttcatct tccggtggca ggatacagcg gataacggtg
      301 tgttcatcgg ggtagtggta ttcaatggcc cgtcggccct gagtggtgac attcacccgc
      361 ccgcgttcat catagccggt gcgctgtgat gtaccgtccg ggctgaagat acccgtcagc
      421 ctgccggatt tgtcatacgc atactgcgtt ttgccttccg gcgtgatgat ttctgccagg
      481 cggcctgcgg cgtcccggct gtagaggata gtttcctcca ggccgttaac cctggcggta
      541 cacaggccat cggcgtcata gcggtaacgc cagaccacgc cgccccagtc atgctcttcc
      601 gtcacccggc cacagttatc cagaacataa aggtatgatt cgccctgcgg gttaatgact
      661 tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc agcggtggcc ttcaccgtcc
      721 gttcg
//
LOCUS       DAZFZC010000381          724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFZC010000381 DAZFZC010000000
VERSION     DAZFZC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.396"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..489
                     /locus_tag="ACHKXP_005521"
     CDS             <1..489
                     /locus_tag="ACHKXP_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650268.1"
                     /translation="SLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            567..>724
                     /locus_tag="ACHKXP_005522"
     CDS             567..>724
                     /locus_tag="ACHKXP_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7650269.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACL"
BASE COUNT          231 a          168 c          175 g          150 t
ORIGIN      
        1 agcctgatga aatgtggcat aggcgacgat gactataacg ggcagaaggc gtttgttgac
       61 tcgatttgcg acttcaaaaa caaaacaaac tcccacgtca ttctcgttac tcactcgcga
      121 aaaggagaca gcgaagaaaa accaaccggg aaaatggacg taaaaggctc tggagcgata
      181 acagacctga cagacaacct tttcatcatc tggcgtaaca aggctcgcga gagagcgtta
      241 cagagagttc agagtggtga aaagatgtca gagaaggacg aacagctact ggcatctccg
      301 gcatctgttt tgatgcttga aaaacaacgt aacggcgaag gttgggaagg tggtgtcccg
      361 ttgttccttg acgagcaatc gcaccagttc ctgcaacttg aatcaggatc gccatatagc
      421 tacatcgcca atatgccgaa atcggaatat gacgaggcgt ggcgacagga aaacgtgaca
      481 gagtattaaa tgaccatcta catcactgag cttgtaacag gcctgctggt aatcgcaggc
      541 ctttttattt gggggagagg gaagtcatga aaaaactaac ctttgaaatt cgatctccag
      601 cacatcagca aaacgctatt cacgcagtac agcaaattct tccagaccca accaaaccaa
      661 tcgtagtaac cattcaggaa cgcaaccgca gcttagacca aaaccgaaag ctttgggctt
      721 gcct
//
LOCUS       DAZFZC010000382          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFZC010000382 DAZFZC010000000
VERSION     DAZFZC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.397"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..492
                     /locus_tag="ACHKXP_005523"
     CDS             <1..492
                     /locus_tag="ACHKXP_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFO7650270.1"
                     /translation="VNEKNNTQQIKCVIFNSLRALGHDKENSLKRIINSFNSELMGEM
                     SNNNIKVHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESNSDSAPYTLLKN
                     YADRFKKYSAEEQYEIDFQHRQVNRNCYLDKYGDANINDYYRDLEIKHSQPQNRASGK
                     RVS"
BASE COUNT          255 a          131 c          127 g          199 t
ORIGIN      
        1 gtaaatgaaa aaaataatac acaacaaata aaatgcgtca tattcaactc attgcgtgca
       61 ctaggccatg ataaagaaaa ttcacttaag cgtattatta atagtttcaa ttctgaactc
      121 atgggagaaa tgtcgaataa taatataaaa gttcatttaa ctgaacccga gataatattt
      181 ttacatgccg atcttcagca atatttaagc caaagttgcg gtgcatttgt gtgcatggca
      241 gcccaggaag tgattgaaca aagggaaagc aattctgaca gcgcccccta tacgttatta
      301 aaaaactatg ctgacagatt taaaaaatat tcagcagaag agcagtacga aattgatttt
      361 caacatcgac aggtaaacag aaattgttat ttagataaat atggcgatgc aaatatcaat
      421 gattattata gagacttaga aataaaacac tcacaacccc aaaatagagc atcaggcaaa
      481 agagtaagtt aacatcatag ttaattaatt cgaccagtca gcaaatatgc tgactggtcg
      541 aacatagttt gctaaaaagt catcactgac tgatgtcagt cacaccatcc gccagttcaa
      601 tctacgggta tttccagcgt atatcggcac gcgattgctg gtctgttgat gtagtggatt
      661 gtagttgctg acttttctta ccctcagatt tctgccccac ttgtcaattc aa
//
LOCUS       DAZFZC010000383          707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFZC010000383 DAZFZC010000000
VERSION     DAZFZC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.398"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            112..>707
                     /locus_tag="ACHKXP_005524"
     CDS             112..>707
                     /locus_tag="ACHKXP_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFO7650271.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          179 a          144 c          178 g          206 t
ORIGIN      
        1 gccacatcac ttcgtgtgtg gttatctttt tatctattgg gctaattttg accgattgag
       61 gtttcctata ggtattcatt caaatatatc tcagtcagga gtactactat tgtgagtaaa
      121 cgtcgttatc ttaccggtaa agaagttcag gccatgatgc aggcggtttg ttacggggca
      181 acgggagcca gagattattg tcttattctg ttggcatatc ggcatgggat gcgtattagt
      241 gaactgcttg atctgcatta tcaggacctt gaccttaatg aaggtcgaat aaatattcgc
      301 cgactgaaga acggattttc taccgttcac ccgttacgtt ttgatgagcg tgaagccgtg
      361 gaacgctgga cccaggaacg tgctaactgg aaaggcgctg accggaccga cgctatattt
      421 atttctcgcc gcgggagtcg gctttctcgc cagcaggcct atcgcattat tcgcgatgcc
      481 ggtattgaag ctggaaccgt aacgcagact catcctcata tgttaaggca tgcttgcggt
      541 tatgaactgg cggagcgtgg tgcagatacc cgtttaattc aggattatct cgggcatcga
      601 aatattcgcc atactgtgcg ttataccgcc agtaatgctg ctcgttttgc cggattatgg
      661 gaaagaaata atcttataaa cgaaaaatta aaaagagaag aggttta
//
LOCUS       DAZFZC010000384          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFZC010000384 DAZFZC010000000
VERSION     DAZFZC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.399"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(98..571)
                     /gene="dfrA5"
                     /locus_tag="ACHKXP_005525"
     CDS             complement(98..571)
                     /gene="dfrA5"
                     /locus_tag="ACHKXP_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA5"
                     /protein_id="HFO7650272.1"
                     /translation="MKVSLMAAKAKNGVIGCGPHIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSAWTADNDNVIVFPSIEEAMYGLAELTDHVIVSGGGEI
                     YRETLPMASTLHISTIDIEPEGDVFFPNIPNTFEVVFEQHFSSNINYCYQIWQKG"
BASE COUNT          174 a          171 c          161 g          196 t
ORIGIN      
        1 attatgtgct tagtgcatct aacgccgtta tcaattgcgg taagaagcgt agcaagcgaa
       61 gcgaacgaag ctttttaccg tcaattgcat agctttgtta acccttttgc caaatttgat
      121 agcaatagtt aatgtttgag ctaaagtgtt gctcaaaaac aacttcgaag gtattgggaa
      181 tattcggaaa gaaaacatct ccttccggct caatatcaat cgtcgatata tggagcgtag
      241 aggccatggg caatgtttct ctgtaaatct ccccgccacc agacactata acgtgatcgg
      301 tgagttcagc cagcccgtac atggcctctt cgatcgacgg gaatactatt acgttgtcat
      361 tatcggccgt ccaggctgag cgagtaacga ccgcgtattt cctattaggg agtgctccca
      421 tagattcgaa cgtcttgcgg cccaccaaaa gccactggtt gtacgtcaag gctttaaaga
      481 gtagctgctc tcctttcgcg gaccagggta tgtgtggacc gcaaccaatc actccgtttt
      541 tcgcttttgc agccattaat gatactttca caattttggt tccgggttaa ctttgtttta
      601 gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
      661 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gt
//
LOCUS       DAZFZC010000385          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFZC010000385 DAZFZC010000000
VERSION     DAZFZC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.400"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..615
                     /locus_tag="ACHKXP_005526"
     CDS             <1..615
                     /locus_tag="ACHKXP_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7650273.1"
                     /translation="GQWRDGESGLCYNRFRYYDPETGMYLVSDPLGLQGGEQTYRYVP
                     NPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTGFLDGVRLSRSQINNIAKEMEKLG
                     IKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLYEVYHEVKNVNKGVGLVGDNGIVL
                     VIRSSNSKNTLEEFHEFFGNFKPNNSTARVEVIDQSVLEPFWRF"
BASE COUNT          215 a          105 c          177 g          201 t
ORIGIN      
        1 ggacagtggc gggatggaga atccgggctg tgctataacc ggttccggta ttacgatccg
       61 gaaaccggaa tgtacctggt gagtgatccg ctggggttgc aggggggtga gcagacttac
      121 cggtatgtgc cgaatccttg tgggtgggtt gatccgctgg gattggctgc aagttctaaa
      181 atcagcagct tgatggacta tattggcgat ggtcgtcgtg ttagtgggca tacgggtttc
      241 ctggatgggg ttcgtttatc acgtagtcaa ataaacaata ttgctaaaga aatggagaag
      301 ctaggaatta aagtaataag gaaagcagat aaatatttgc caccaaatgc tagggcagct
      361 tttgattatg gtcttcgcaa tatttatctt aggaaaaatg ctaccttata tgaggtatat
      421 catgaggtaa aaaatgttaa caagggagtt ggcctggtgg gagataatgg tatcgtcctg
      481 gtaattagaa gttccaattc caaaaataca cttgaggaat ttcatgaatt ctttggaaac
      541 ttcaaaccta ataattcaac agctcgagtt gaagtgattg atcaatccgt tttagaacct
      601 ttttggagat tttaaaaata tgatcaccaa atcaagtata ctcatagagt ataaagataa
      661 aagtaaacta accgatagta tttataagtt tgtaaatg
//
LOCUS       DAZFZC010000386          689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFZC010000386 DAZFZC010000000
VERSION     DAZFZC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.401"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..229
                     /locus_tag="ACHKXP_005527"
     CDS             <1..229
                     /locus_tag="ACHKXP_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7650274.1"
                     /translation="QMNDDKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVTRENTTS
                     ETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            245..>689
                     /locus_tag="ACHKXP_005528"
     CDS             245..>689
                     /locus_tag="ACHKXP_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650275.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIE"
BASE COUNT          200 a          143 c          167 g          179 t
ORIGIN      
        1 acaaatgaac gacgataaaa atataaaact gctagcgatt aacggtattg caccgactgc
       61 cgaaaacatt cgcaacggca aatatcccta tatcgtcgat gcctttatgg taacgcggga
      121 aaatacgacg tcagagacac aaaaattgtt ggagtggttt ttaacgccgc agggacagag
      181 tttggtggaa gatgtaggtt atgtcccgat gtataagacg ctaccttaaa gctggataat
      241 aaaaatggaa ttagttaata cattatttgc aagccttgcc ggtactgatc ccttcacagg
      301 cgtggatatt acgattgcca actgcaaatc ggcatactgg gatgaaggca ttgttcagca
      361 actcattaac caggccctgg atgaagggga aaagttcgtc ggtgctgacg gtcttgaggg
      421 attgcttcga tataatgtca cattaaatat tggcttaacg tccagcaatg tatggccggg
      481 attttccctc gataccgcca ccattagccg tctttgtgcc tgtggtgctg actttggatt
      541 tgacccctat atcagtgatg taccagatgt tcaatgtgat ctgaacacga caaatgacct
      601 caccgttcaa tttacggcga tgctaaaccc ggatgagcgg gtgatcatag caaagcgccc
      661 gcttaaaaaa tgtgagtcct ggattgagg
//
LOCUS       DAZFZC010000387          688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFZC010000387 DAZFZC010000000
VERSION     DAZFZC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.402"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..620)
                     /locus_tag="ACHKXP_005529"
     CDS             complement(<1..620)
                     /locus_tag="ACHKXP_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7650276.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          168 a          174 c          167 g          179 t
ORIGIN      
        1 ggcaccacgc cgatggtgtt ttcgtcacag gcttcaatca tgcgtttcgg gtccataaac
       61 aactgaccgg ggcgcatagg gatctcacgc agctccacat cccagtagcg ggcgaattta
      121 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgttgg tttgcctgca
      181 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      241 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggcgcagg cgcatgccac
      301 agatcggcaa ccatatttac gcaacgcagg tcgatggctg cggattgcgg atattcttct
      361 ttgtcgatcc agtttttatt gatcgacaaa tccatcaatt tatggacgtt ttcgtcgtcc
      421 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcatca
      481 ttgataatct gaaatgcgac atcatcgcgc atttcgtgca gcggaaatcg ttttgactcc
      541 gcgatagtag aaatggcctt tgcgccaaaa cgtgaatcga gtagttctga gcggaaatcc
      601 gttaacagct tctggtccat ttcgaactcc ttaaatttat ttgaaggcaa taaaaaagta
      661 ggatttatcc gcaatggaag caaggcat
//
LOCUS       DAZFZC010000388          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFZC010000388 DAZFZC010000000
VERSION     DAZFZC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.403"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>686
                     /locus_tag="ACHKXP_005530"
     rRNA            <1..>686
                     /locus_tag="ACHKXP_005530"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          155 c          226 g          130 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAZFZC010000389          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFZC010000389 DAZFZC010000000
VERSION     DAZFZC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.405"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..335
                     /locus_tag="ACHKXP_005531"
     CDS             <1..335
                     /locus_tag="ACHKXP_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7650277.1"
                     /translation="AGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPAS
                     IIETNSNGWFPDTDGALITGLTFLDPKDATQVQGLFRHLQVRFGDGPWQDVKGLDEVG
                     SDTGRTGE"
     gene            337..606
                     /locus_tag="ACHKXP_005532"
     CDS             337..606
                     /locus_tag="ACHKXP_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7650278.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQLLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          170 a          148 c          218 g          125 t
ORIGIN      
        1 cggcaggccc ggcaggccca cagggaccga aaggagaaac aggtgcggct ggcccggtgg
       61 gggcaaccgg acctcaggga ccaaagggcg acccggggga gacacaaatc cgttttcgtc
      121 tggggccggc gagcattatt gagacaaaca gcaatggctg gttcccggat acagatggcg
      181 cactcatcac cggactgacc tttcttgacc ccaaagatgc cacacaggtt caggggctgt
      241 ttcggcattt gcaggtcagg tttggtgacg ggccgtggca ggatgttaag gggctggatg
      301 aagtgggcag tgatacaggc agaacaggag aatgacatga atatactaaa aaaacttatg
      361 cagcgtctgt gtggttgcgg aaagcatgat ggccgtgaac acgtgcagtt gcttacagca
      421 caactgcgtc tgggaccggc agacattctg gagtcagatg agaatggcat tattccggag
      481 caggacaggg taatcacgca ggtggtgata ctggatgcag ataaaaagca gatacagtgc
      541 gtggtaagac cgctgcaaat tctgcgtgct gacgggacgt gggaaaatat tggcgggatg
      601 aagtaacccg acagcttcac aaaaccggag tccggctccg gtttttgttg gcatgccatg
      661 g
//
LOCUS       DAZFZC010000390          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFZC010000390 DAZFZC010000000
VERSION     DAZFZC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.406"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>657
                     /locus_tag="ACHKXP_005533"
     CDS             <1..>657
                     /locus_tag="ACHKXP_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7650279.1"
                     /translation="EKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGP
                     AGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKA
                     VKAAMDVANEAKTKAEEAAAGGGVPGPKGDKGDTGPAGPAGPKGDKGERGDTGPVGAT
                     GERGPAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETG
                     "
BASE COUNT          172 a          155 c          265 g           65 t
ORIGIN      
        1 gcgagaaagg tgatccggga ggtcctgcag gcccgcaagg tcctaaaggc gaacgaggag
       61 aagccggacc acagggaccg atgggagcac gaggtgagcg tggggagact ggcccccgag
      121 gtgaacctgg tcctgcaggt ccgagaggcg aacgaggaga gaccggacct cagggacctc
      181 gtggagagcc aggtccggca ggcagcgctg caaatgtggc tgatgcaacg acggcacaga
      241 agggaattgt gcagttaagc agcgcaacgg acagtgatga tgaaacgaag gctgccaccc
      301 cgaaagcggt gaaagcggca atggatgtgg caaatgaagc gaaaacaaag gcagaagagg
      361 ctgcagcagg aggtggtgtt cccggtccga aaggagataa aggggacacg gggccagcag
      421 gtccggctgg gccgaagggt gataagggag agcgcggtga caccggccct gtcggggcaa
      481 ccggcgaacg gggaccggca ggtgatgctg gtccggcagg cccgcagggg ccgaaaggtg
      541 acaggggaga gcggggagag accggtctga cgggaaatgc aggtccacag ggtccaaagg
      601 gagataccgg tgcggcaggc ccggcaggcc cacagggacc gaaaggagaa acaggtg
//
LOCUS       DAZFZC010000391          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFZC010000391 DAZFZC010000000
VERSION     DAZFZC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.407"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..187
                     /locus_tag="ACHKXP_005534"
     CDS             <1..187
                     /locus_tag="ACHKXP_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO7650280.1"
                     /translation="PLFVKTEAIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTG
                     ALIDESLVEKANKFWGI"
     gene            complement(209..544)
                     /locus_tag="ACHKXP_005535"
     CDS             complement(209..544)
                     /locus_tag="ACHKXP_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HFO7650281.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
BASE COUNT          215 a          140 c          109 g          192 t
ORIGIN      
        1 tcctcttttt gttaaaacag aagccattat agcaggcaat gccgcaacgg ctcttgttgc
       61 actggtcttc agtattctta ccggaagcgc tttaggcatt atcgggtatg gtttactgat
      121 ggctgtcacc ggtgcgctga ttgatgaatc gcttgtggaa aaagcgaata agttctgggg
      181 tatttaaata aaatataaga caggctgtct atcttacaga cagccttttt ataataacag
      241 aaagaatata tatcatactt aacggaagag ataatatgaa aacagtaccc gtataaacag
      301 caagcaatcc catttttcca ggtgtatcag caaaaaaacc gctgttccag aaatcaggcc
      361 gggtaaattt cagagctgta tcttcaatat accattttgc aaccggatac aaaaccattc
      421 cgcagagaga aatacaccag aacagtaatc tgtatttaaa atcataatcc catgacatat
      481 acagcatata tccccccgtc acccatcccc accacatatt attaaaataa tattttctgt
      541 tcatctgcct gttttccttt cttttgcact tttgtcagta tactgatgca tgacaacata
      601 accacagcat gataataata atcaataaca ataagctgtg tcacgtttac atcatg
//
LOCUS       DAZFZC010000392          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFZC010000392 DAZFZC010000000
VERSION     DAZFZC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.408"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>648
                     /locus_tag="ACHKXP_005536"
     CDS             <1..>648
                     /locus_tag="ACHKXP_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7650282.1"
                     /translation="QSRSQRRGKTASFRANLQGAWNDRENARLGLAAATLQSDMEKAG
                     ELAARDRAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNT
                     LMAAAKKDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKIS
                     QQRRDLWKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQ"
BASE COUNT          187 a          163 c          210 g           88 t
ORIGIN      
        1 cagagccgga gccagcgccg gggaaaaacc gcctctttcc gggccaacct tcagggcgca
       61 tggaatgacc gggaaaatgc ccgtctgggg ctggcagcgg ccacgctgca gtcggatatg
      121 gaaaaagccg gtgaactggc cgccagggac cgggccgaac gggacgcatc acagctgaag
      181 tataccggag aggcgcagaa ggcgtatgag cgtctgctga cgccgctgga gaaatatacc
      241 gcccgtcagg aagaactgaa taaggccctg aaagacggga aaatcctgca ggcggattac
      301 aacacgctga tggcggcggc gaaaaaggat tatgaatcga cgctgaaaaa gccgaagtcg
      361 tcaggagtca aagtgtcagc cggtgagcgt caggaagacc aggcgcatgc tgccctgctg
      421 gcgcttgaaa ccgagctccg gacgctggaa aaacacagcg gtgcgaatga gaaaatcagc
      481 cagcagcgtc gcgatttatg gaaagcggaa aatcagtatg cggtcctgaa agaggcagcc
      541 acgaaacggc agttatctga gcaggaaaaa tccctgctga cccatgagaa agagacgctg
      601 gagtacaaac gccagctggc tgagctggga gacaaagttg aacaccag
//
LOCUS       DAZFZC010000393          644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFZC010000393 DAZFZC010000000
VERSION     DAZFZC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.409"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            273..>644
                     /locus_tag="ACHKXP_005537"
     CDS             273..>644
                     /locus_tag="ACHKXP_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650283.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     NNNYQFIVTINSKNYGCSLDNT"
BASE COUNT          219 a          101 c          124 g          200 t
ORIGIN      
        1 aaccacatta aaactccatg aatacggtgg tatgtctttt taatcctgcc agtatttcat
       61 aggttttctt aatcgattgt agaggcatgt tctgatgacg cctctacaat ctttagggac
      121 tttctctgct atgagataag aaatttcata ttatgctttg aggctgaatg atttgtgcaa
      181 aatataatta ttttcccatc gaacatgagg tgtatgtcat aagataaaat gttaactatg
      241 tattcctcgc ggtaaataat atgaggtaaa atatgaaaat tggaactgtg gcaggcacta
      301 acgacagtac aacgacaatc gcaacaaatg atatggtgca agagcatgtt accaacttca
      361 ctaaagaact gtttgggtat attgcaaatg gtataggtga tgatatatca agtattgcca
      421 ggacaatgct cggtgaggtt gtggaaaaaa tagatgattg gcaaatagaa cggttccaac
      481 aatctattca agatgacaaa atttcattta ccatacaaac ggatcattca gaaaaatata
      541 gtatgctatc gggcatgcgt gctcatattc tacgtaggaa taataattat cagtttattg
      601 tgacaattaa tagcaaaaat tacggttgct ctcttgataa tacc
//
LOCUS       DAZFZC010000394          638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFZC010000394 DAZFZC010000000
VERSION     DAZFZC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.410"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>638
                     /locus_tag="ACHKXP_005538"
     CDS             <1..>638
                     /locus_tag="ACHKXP_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7650284.1"
                     /translation="FLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFW
                     EQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWLGWGHAWVHETA
                     VVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQ
                     ILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPLMAWCVG"
BASE COUNT          120 a          141 c          244 g          133 t
ORIGIN      
        1 tttctgtacc gggagtaccg taaagcccgg gaggctggtg aggaagcatt tcgtggcttc
       61 atgtcaaaac acgccaatgt ggaaatcggt cttgccctgc gttctgaccg ctgggcgggc
      121 gcggatttct gggagcagca gggcaggcgc gtcagcctgg acgatatcct gcagcgcgct
      181 gatgtggtga cggtggggat tgacggcggg ggcctggatg atctgctggg aatgtacgtg
      241 actggccgtg acagggaaac ccgcgaatgg ctgggctggg gccatgcctg ggtgcatgaa
      301 accgcggtgg tcagacggaa gagtgaggca tcccggtttc aggattttgt ggcctgtgga
      361 gacatgacga ttgtccgtcg ggtcggggat gacacggcgg aagtggcgga gtatgtgcgt
      421 cgtattcatg aggctgagtt actggatcat atcggtattg acccgtcagg tgtggggcag
      481 attctggatt cactggcgga agccgggatc cccgatgaga gtgtggtggg gataagccag
      541 ggctggaagc tgggcggggc catcaaaacc accgagcgca aactggctga aggggtgctg
      601 attcacggtg atcagcccct gatggcctgg tgtgtcgg
//
LOCUS       DAZFZC010000395          613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFZC010000395 DAZFZC010000000
VERSION     DAZFZC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.411"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            188..367
                     /locus_tag="ACHKXP_005539"
     CDS             188..367
                     /locus_tag="ACHKXP_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7650285.1"
                     /translation="MTFIHQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            408..>613
                     /locus_tag="ACHKXP_005540"
     CDS             408..>613
                     /locus_tag="ACHKXP_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7650286.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSAS"
BASE COUNT          165 a          141 c          168 g          139 t
ORIGIN      
        1 ctgattgaca gcattgcggt ggaagtgaac aacgcggatg ccgcggcatg ataaaaaaag
       61 ccgccagttg cctgagacct ctggcggtgt gagattcatg gagaatcaag gaaagatact
      121 accactttcg tcatcggcat ttttaaatga aaactgttta cttagtcaac cataacggta
      181 agaaattatg acatttattc atcagctgat gctgtacttc tgtacggtgg tctgtgtgct
      241 gtatctcctt tcgggtgggt acagggccat gcgtgacttc tggcgcagac agattgacaa
      301 aagggccgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagagccgct
      361 catttagcgg caattttctt aatcacatct ttcgacgaga aaatcccatg tcagaaatta
      421 catccctggt cactgctgaa gcagtgaagg aagtcctgcg ctctgaagaa gtccggagcg
      481 cactgaaaca gaaacttcgc cataacctgg aagcgcgtct tgatgcagaa gtggatgcca
      541 ttctggatga actgctgggc gcaccggcag ctccggagcc ggaaggcatc gcgggtgagg
      601 ggagtgcttc aga
//
LOCUS       DAZFZC010000396          612 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFZC010000396 DAZFZC010000000
VERSION     DAZFZC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.412"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            187..366
                     /locus_tag="ACHKXP_005541"
     CDS             187..366
                     /locus_tag="ACHKXP_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO7650287.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            407..>612
                     /locus_tag="ACHKXP_005542"
     CDS             407..>612
                     /locus_tag="ACHKXP_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7650288.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSAS"
BASE COUNT          173 a          137 c          172 g          130 t
ORIGIN      
        1 ctgatagaca gcattgcggt ggaagtgaac agcacagaca ctgcggcatg ataaaaaaaa
       61 ccgccagcga caggaatgga cgctggcggt ggtgatacct atggagaaaa aataaaggaa
      121 cgatactttc gtactctggt ttttaatgaa aacagttctt attgtcaaca ataacggaaa
      181 gaaattatga catttctgaa ccagttaatg ctgtacttct gtacggtggt ctgtgtgctg
      241 tatctccttt cgggtgggta cagggcagtg cgcgattgct ggcgcaggca gattgacaaa
      301 agggccgctg agaaaatcag cgccggtcag tcagccggaa gcaaacccga agagccgctc
      361 atttagcggc aactttctta atcacacctt tcgacgagaa aatcccatgt cagaaattac
      421 atccctggtc actgctgaag cagtgaagga agtcctgcgc tctgaagaag tccggagcgc
      481 actgaaacag aaacttcgcc ataacctgga agcgcgtctt gatgcagaag tggatgccat
      541 tctggatgaa ctgctgggcg caccggcagc tccggagccg gaaggcatcg cgggtgaggg
      601 gagtgcttca ga
//
LOCUS       DAZFZC010000397          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFZC010000397 DAZFZC010000000
VERSION     DAZFZC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.413"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>608
                     /locus_tag="ACHKXP_005543"
     CDS             <1..>608
                     /locus_tag="ACHKXP_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO7650289.1"
                     /translation="ARKNGTYSGSQQNTGASDTTAQPGPVKVTADEVNKIMQAANISQ
                     PDADELLAVSRGEFVAGISDPNDPKWVKGIETRDSVNQNQQETEQNDQKAEQNSPNTQ
                     QNEPETKLPEPVVQQEPEKICTACGQSGGGNCPDCGAVMGDATYQETFDDKNQVEVQE
                     DDSEKMEGAEHPHKENAGSALDHASDSETGETADHPIKVNGH"
BASE COUNT          207 a          147 c          162 g           92 t
ORIGIN      
        1 gcccgcaaaa acggtacata ctcaggttca caacaaaata ctggtgcatc tgacacgacc
       61 gcacaaccag ggccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc
      121 aatatcagcc agcctgacgc cgatgaactg cttgcagtat cacgtggtga atttgttgca
      181 gggattagcg atccgaatga tccgaaatgg gtgaagggga ttgaaacccg cgattctgtg
      241 aaccagaacc agcaagaaac ggaacagaac gaccagaaag cggaacaaaa cagcccaaat
      301 acgcaacaaa acgagccaga aacgaaacta cctgaaccag tagtgcaaca ggaaccggaa
      361 aagatctgca ccgcctgcgg tcaaagcggt ggcggcaact gccctgattg tggtgcagtg
      421 atgggcgacg caacatacca ggaaacattc gatgacaaga accaggttga agttcaggaa
      481 gacgattcgg agaaaatgga aggcgctgaa catccacaca aggagaatgc tggcagcgct
      541 ctggatcacg ccagcgatag tgaaactggc gagacggcag atcacccaat taaggtgaac
      601 ggtcatca
//
LOCUS       DAZFZC010000398          601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFZC010000398 DAZFZC010000000
VERSION     DAZFZC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.414"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            166..483
                     /gene="yegR"
                     /locus_tag="ACHKXP_005544"
     CDS             166..483
                     /gene="yegR"
                     /locus_tag="ACHKXP_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO7650290.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
BASE COUNT          188 a           92 c          142 g          179 t
ORIGIN      
        1 agccctcatg gagttacccg gaagggcctc catgtccgta attcctactt atgtaggaaa
       61 tgttgtacag aacatttatt ataatcctat tcaattataa taatcatgcc attattatat
      121 ttaaacacta gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct
      181 gctatatcat taattagtat ttttattatg tctggttgtg ctgtgcataa tgatgagaca
      241 agtatcggta aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa
      301 tactacaccg aagccgaagc ttctttagcc aggggtagaa tatctggtgc agaaaatata
      361 gtaaaaaatg atgcaactca tttctgtgtt actcagggca aaaaaatgca aatagttgag
      421 ctgaagacag aaggtgtagg attacatggt gtcgctcgtc tgacattcaa atgtggagag
      481 tgagaatatt ttttggtaag cgtcaaatat gcgcgttctg gctgtgcgta gccggaacct
      541 gtgggagcac gatgccgata agtgaaaggc atcgtgctat gaaggaggat tctatcgatg
      601 t
//
LOCUS       DAZFZC010000399          591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFZC010000399 DAZFZC010000000
VERSION     DAZFZC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.415"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..279
                     /locus_tag="ACHKXP_005545"
     CDS             10..279
                     /locus_tag="ACHKXP_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650291.1"
                     /translation="MNKKQLAILEKAWDAQISYALKEQALPIIQTKSKIARQLCDGGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEGEMKQ"
     gene            365..580
                     /locus_tag="ACHKXP_005546"
     CDS             365..580
                     /locus_tag="ACHKXP_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650292.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
BASE COUNT          173 a          110 c          154 g          154 t
ORIGIN      
        1 gagtattaaa tgaataaaaa acaattagcc attctcgaaa aggcatggga tgcacaaata
       61 tcatacgctt tgaaagaaca ggcactacca ataatccaga ccaaatcgaa aatagccagg
      121 cagttatgcg atggcggatt cctgaacgaa gttgagatta cgcgccagat ggtaacgttc
      181 aaagggtatg agataaatca tcatggtata gcagcgtatt gttcccatct tcctgatgac
      241 gttgacattg atgaaatgga aggggagatg aagcaatgac catctacatc actgagcttg
      301 taacaggcct gctggtaatc gcaggccttt ttatttgggg gagagggatt ggaggcttta
      361 agaaatgagt acgatagctg agcttgtcag ggctaatttt cgtgaagagt tggtgcgttg
      421 gtatcggtat cgttcatcgt ccagtttgcc gcttgatgag ttgtatgagc attcacctgc
      481 cgcacgacgc tatccgcgtg accgtgttct tcgacggttg ttcaaactca acaatgagtt
      541 tcagcgcaac agaattatcc ggagtctgga tttaaagtga aggagtgagc a
//
LOCUS       DAZFZC010000400          589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFZC010000400 DAZFZC010000000
VERSION     DAZFZC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.416"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>589
                     /locus_tag="ACHKXP_005547"
     CDS             <1..>589
                     /locus_tag="ACHKXP_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7650293.1"
                     /translation="DAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNI
                     TEMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGG
                     AVANAKGSVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLG
                     VRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEA"
BASE COUNT          136 a          130 c          208 g          115 t
ORIGIN      
        1 gatgcgcagc gtgcggactg gcagaaaggg ctgcaggagg gattcagtaa ctgggtggat
       61 aatgcgtccg attacgcctc acaggcagca cagcttgcga cggagggtat ctcagggatg
      121 gtgaataaca tcacggagat gctgaacgga aataaagtgg aatggcgcag ctgggcctca
      181 tcagtgctgc aggaaatatc aaaagttctt atgaatgccg cgattgtcaa cggaattaag
      241 acggcggcaa acggtatgtc cggtgcggga ggatttctcg gcagcattgg tgactggctg
      301 ggcggagcgg tggccaatgc aaaaggcagc gtgtatacct cggcaaacct gagtgcgtac
      361 agcaacagta ttgtggatac gcccacgtac tttgcctttg caaaaggggc cgggctgatg
      421 ggggaggccg gacctgaagc cattatgcct ctgacgcggg cggcggatgg ctcgctgggt
      481 gtgcgcgcgg tgggtagtat gaacggcagt gcgggtctgg tgtattcccc ggtctaccat
      541 atcgccattc agaatgacgg gactaatggc cagatagggc cggaggcgg
//
LOCUS       DAZFZC010000401          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFZC010000401 DAZFZC010000000
VERSION     DAZFZC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.417"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..503
                     /gene="ygeG"
                     /locus_tag="ACHKXP_005548"
     CDS             12..503
                     /gene="ygeG"
                     /locus_tag="ACHKXP_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7650294.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          216 a           91 c          101 g          178 t
ORIGIN      
        1 agaggaatat tatgagcaca gaaacaattg aaatattcaa taatagtgat gaatgggcaa
       61 atcaactaaa acacgcatta tcgaaaggag aaaatctggc attactacat ggtttaactc
      121 ctgatatcct tgatagaata tatgcatatg cattcgacta ccatgaaaag ggtaatataa
      181 cggacgcaga aatttattat aaattcttgt gcatttatgc gttcgaaaat catgagtatc
      241 taaaagattt tgcatcagta tgtcagccca aaaagaaata tcaacaagca tatgaccttt
      301 acaaactaag ttacaattac ttcccgtatg atgactattc agttatttat cgtatgggtc
      361 aatgtcagat tggggctaaa aatatcgata acgcaatgca atgtttctat cacattatta
      421 acaattgtga ggatgatagt gttaagagta aagcgcaggc atatattgaa ctcttaaacg
      481 ataattcaga agataatggc taagcattac aatcatttgg ttaaatttgc tggttatagt
      541 gcagttaacc actataacca gcacctctta tgttttctta aatcag
//
LOCUS       DAZFZC010000402          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFZC010000402 DAZFZC010000000
VERSION     DAZFZC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.418"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          173 a           99 c          118 g          169 t
ORIGIN      
        1 atttcttaat ataaaagatc cattacattg ctctgcaact ataactgcgt attaccgtga
       61 aggagaacgg tgagtaacgg cgtttttcaa ggccattgtc aacgacggat gaaaagtgga
      121 atcctctgct gggattcagc tcctaatgct acaaatctca ttgatggctt gatgtaactt
      181 cacatatggt ttcagcctca aagaaatcat ttttattaat tttaacgaag ggttggacaa
      241 aatgaggagg tattagcttt gttttatgac gcatttccga aaatttccgt gccatatagt
      301 gagcatcttc gcaagcttcc ataacaatag ttgtgtagga ggaattagta aggaattcga
      361 ataatttcga gcacttggat tctgacagtt aaggactctg cctgttgcat cttggcagtg
      421 gacgttgaat gaatttttgc ctagataaat accaatcaaa gatgacgtta ttcataatgg
      481 ttctcccaaa aaaacaccgt cagcgtactg ctgcagggta gggtgatcat ctaataaaat
      541 ccagtataac tggataaga
//
LOCUS       DAZFZC010000403          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFZC010000403 DAZFZC010000000
VERSION     DAZFZC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.419"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(25..>554)
                     /locus_tag="ACHKXP_005549"
     CDS             complement(25..>554)
                     /locus_tag="ACHKXP_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO7650295.1"
                     /translation="EWLGDLTGVNQVSFRRLRESSSTRNQLNKILRQEIQDKISGVEL
                     SDINKEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQLSFY
                     WQGGQCKPEYGEERFCSELIKYGAGNLEWLFSDNPLWTIVKYLLPKSGEIKPTHINDL
                     FLNRLNKILLPYETL"
BASE COUNT          180 a          105 c           71 g          198 t
ORIGIN      
        1 aaatcaattt ttatattttt atattcataa agtttcataa ggcaataaaa ttttattaag
       61 tctgttaaga aataaatcat taatatgggt tggttttatt tctccacttt tcggtaaaag
      121 atatttgact atagtccata agggattatc agaaaatagc cattccagat ttcctgcacc
      181 gtattttatc agttcagaac aaaaccgttc ttcgccatat tctggtttac attgccctcc
      241 ctgccaatag aatgataact gcttcacatt ttgatatcct atctctctga tcagagtttc
      301 tcgatataat cgtgaaaaaa tatagtcagg gcattgaatt tcaaataatg cactacttag
      361 agcaagcaat cttattttcc caattgactg atataaaaaa ccttccttat ttatatcaga
      421 taattccact ccagaaattt tatcttgtat ttcctgacgc aatatcttat tgagttgatt
      481 acgagtagaa ctactttctc ttaaacgccg aaaactaacc tgattgacac cggttaaatc
      541 acctagccat tcat
//
LOCUS       DAZFZC010000404          554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFZC010000404 DAZFZC010000000
VERSION     DAZFZC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.420"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..251)
                     /locus_tag="ACHKXP_005550"
     CDS             complement(<1..251)
                     /locus_tag="ACHKXP_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7650296.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTL"
     gene            293..478
                     /locus_tag="ACHKXP_005551"
     CDS             293..478
                     /locus_tag="ACHKXP_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7650297.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQLNIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
BASE COUNT          156 a          158 c          118 g          122 t
ORIGIN      
        1 cagagcgtcc agagattcgt ctcctcatta ccaccaccga actgaagtgc aatgcggtga
       61 tcgagttcac tgtcacagag gtcaaccaca cgaccacaga gacagcactg cccggcatcc
      121 ctcagccaga tatgacgctt gagggaaacc cgggcactgc cactgacacg acgctgttcc
      181 cccttcagaa tattcacccg tcgggtattc agtgttttga ttctgctctg gagtgtacga
      241 agctcagcca tgtaaaatcc ccgtcatata acttgtcacc agaggaaaga aaatgtcatc
      301 gaaaaaccgg acccgcagaa ccacaacccg caatatccgt ttccccaatc acataattga
      361 acagctcaat atcgcccttg aacataaagg ttccggtaac ttttcagcgt gggttattga
      421 agcctgcaga agaaggctgt caacagagcg ttcgggtatg aattacataa ttaagtaaca
      481 tggtgttcac agaacacaca gttaccggac acatcagttt tccattcgtc ccccggcagt
      541 acaggcttcc cgtc
//
LOCUS       DAZFZC010000405          552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFZC010000405 DAZFZC010000000
VERSION     DAZFZC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.421"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>552)
                     /locus_tag="ACHKXP_005552"
     CDS             complement(<1..>552)
                     /locus_tag="ACHKXP_005552"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO7650298.1"
                     /translation="ALKADGIPVSLDSYQPATQAYALSRGVAYLNDIRGFPDAAFYPQ
                     LAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGAGIKRNRLVLDP
                     GMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRGPGDVGAATLA
                     AELAAAAGGADFIRTHEPRPLRDG"
BASE COUNT          126 a          168 c          174 g           84 t
ORIGIN      
        1 ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc tgcggcggcg
       61 gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc tgtgagcgcg
      121 cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg cagccgcaat
      181 tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag aaaaaacccc
      241 atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag cgccgcgatg
      301 cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc ctcgcgccga
      361 tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga tttcgccaat
      421 tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc cacaccacgc
      481 gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg aatgccatct
      541 gccttgagcg cg
//
LOCUS       DAZFZC010000406          552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFZC010000406 DAZFZC010000000
VERSION     DAZFZC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.422"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            100..>552
                     /gene="nleB2"
                     /locus_tag="ACHKXP_005553"
     CDS             100..>552
                     /gene="nleB2"
                     /locus_tag="ACHKXP_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO7650299.1"
                     /translation="MLSPIRTTFHNSVNIVQSSPCQTVSFAGKEYELKVIDEKTPILF
                     QWFEPNPERYKKDEVPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFS
                     KLERDFENVMIIYREDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYL"
BASE COUNT          197 a           73 c          118 g          164 t
ORIGIN      
        1 gatggcaaaa ctgctggagg ttttgtggtt gagtatgcca atataattaa tagattaaag
       61 agttagttgt gaagaaaata tggataaaca ggacgacgaa tgctttcacc gataaggaca
      121 actttccata actcagtaaa tatagtgcag agttcaccct gtcaaacggt ttcttttgca
      181 ggaaaggaat atgagttaaa ggtcattgat gaaaaaacgc ctattctttt tcagtggttt
      241 gaacctaatc ctgaacgata taagaaagat gaggttccaa tagttaatac taagcagcat
      301 ccctatttag ataatgtcac aaatgcggca aggatagaga gtgatcgtat gataggtatt
      361 tttgttgatg gcgatttttc agtcaaccaa aagactgctt tttcaaaatt ggaacgagat
      421 tttgaaaatg taatgataat ctatcgggaa gatgttgact tcagtatgta tgacagaaaa
      481 ctatcagata tttatcatga tattatatgt gaacaaaggt tacgaactga agacaaaaga
      541 gatgaatact tg
//
LOCUS       DAZFZC010000407          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFZC010000407 DAZFZC010000000
VERSION     DAZFZC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.423"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..256)
                     /locus_tag="ACHKXP_005554"
     CDS             complement(<1..256)
                     /locus_tag="ACHKXP_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650300.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFMELMDIINKGFGLLMKKKG"
     gene            complement(268..420)
                     /locus_tag="ACHKXP_005555"
     CDS             complement(268..420)
                     /locus_tag="ACHKXP_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7650301.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LTRNTD"
BASE COUNT          124 a          119 c          100 g          149 t
ORIGIN      
        1 tccctttctt tttcattaac aagccaaatc ctttattaat gatgtccatt aattccatga
       61 agtatttttc atgtaaatcc tggttatcag agagctgctt ctcttcgtac agaccgataa
      121 aggcacgacg cacgttaccg gatatagtat cgatggtttc tttttctacg gtactcaggt
      181 caagagtcgc cagttgagag cgaaccacat tcgatgccat ttcctggaat ggtactggta
      241 aatctttaaa ttccatcgtc aacctcatca gtcagtgttt ctggttaacc agcgacgcgc
      301 gccagcttca gttttaaacg ttttgctttt ggtatacgtc atcgcggtga acgtaccgtc
      361 ctggttgggg aacacgccac ataccagaga ttcgctgttg ccaagatcga tagtatccat
      421 gctgacctca tttcccctta acgccggggt agcggaacaa aaacctgctg catagttatt
      481 aaagttgaac cc
//
LOCUS       DAZFZC010000408          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFZC010000408 DAZFZC010000000
VERSION     DAZFZC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.424"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>490
                     /gene="espX1"
                     /locus_tag="ACHKXP_005556"
     CDS             <1..>490
                     /gene="espX1"
                     /locus_tag="ACHKXP_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO7650302.1"
                     /translation="KGITLLRLDVFDDRTSECLSEEIKIYQECHEKFVKFLKANFNHE
                     IYPQLYTPEIFYEACRNLQSFYAHQETSQNAKYSAPDKKKSHLNQEIKKLIKKNIFPE
                     LYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAYSQLCKKLSLFKSIPSRLIEKSAYCST
                     ENT"
BASE COUNT          198 a           90 c           71 g          131 t
ORIGIN      
        1 caaaggcatc acattactta gattggatgt ttttgatgac agaacaagcg aatgtctttc
       61 tgaagagatt aagatatatc aagaatgcca tgaaaaattc gttaagtttt taaaggcaaa
      121 cttcaaccat gaaatatacc cacagctata tactcctgaa atattttatg aggcctgcag
      181 aaatctccag tcattttatg ctcatcagga gacatcacaa aacgctaaat atagtgcacc
      241 agacaagaaa aaaagtcatc ttaaccagga aatcaaaaag ctgattaaaa aaaatatttt
      301 ccctgagcta tacaatgagc aatgcaataa aatcccctcc tcatcgatag atgataacca
      361 gggaataatg tggcagaact ttaaaactag caatattgca tattcacaac tatgtaaaaa
      421 attatcttta tttaaaagca tacccagtcg attaatagaa aaatcagctt attgctcaac
      481 tgaaaacaca
//
LOCUS       DAZFZC010000409          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFZC010000409 DAZFZC010000000
VERSION     DAZFZC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.425"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHKXP_005557"
     rRNA            <1..>476
                     /locus_tag="ACHKXP_005557"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFZC010000410          463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFZC010000410 DAZFZC010000000
VERSION     DAZFZC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.426"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>463
                     /locus_tag="ACHKXP_005558"
     CDS             <1..>463
                     /locus_tag="ACHKXP_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO7650303.1"
                     /translation="RTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELK
                     ATKDAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADIN
                     KKINAKDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGK"
BASE COUNT          172 a           78 c          117 g           96 t
ORIGIN      
        1 agaacaaatg agcaaaagca tgcaaatgac gctcttaatg ccctgttgaa ggaaaaagag
       61 aatatccgta accagcttgc cggcatcaat cagaagatag cggaagagaa aagaaaacag
      121 gatgaactga aggcaacgaa agacgcaatt aatttcacaa cagagttcct gaaatcagtt
      181 tcagaaaaat atggtgcaaa agctgagcag ttagccagag agatggccgg gcaggctaaa
      241 gggaagaaaa tacgtaatgt tgaagaggca ttaaaaacgt atgaaaagta ccgggctgac
      301 attaacaaaa aaattaatgc aaaagatcgt gcagcgattg ccgcagccct tgagtctgtg
      361 aagctgtctg atatatcgtc taatctgaac agattcagtc ggggactggg ttatgcagga
      421 aaatttacaa gtcttgctga ctggatcact gagtttggta agg
//
LOCUS       DAZFZC010000411          458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFZC010000411 DAZFZC010000000
VERSION     DAZFZC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.427"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>458
                     /gene="espX1"
                     /locus_tag="ACHKXP_005559"
     CDS             <1..>458
                     /gene="espX1"
                     /locus_tag="ACHKXP_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO7650304.1"
                     /translation="HKMLLSNIIIEKSNLSYGYYFGCVLSNISCFESDLSNTIFSNGE
                     INNLFIKKSNIFGTSFTNTRIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASID
                     DKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLK"
BASE COUNT          176 a           80 c           72 g          130 t
ORIGIN      
        1 tacataaaat gttgctatca aatataataa tagaaaagag caacctttca tatgggtatt
       61 attttggttg tgtattatcc aatatatcat gttttgaaag cgacttatca aacaccatct
      121 tttcaaatgg agaaataaac aacctcttca ttaaaaaaag caacatattt ggaacatcgt
      181 tcactaatac caggataaaa aatttgcgct gtgaagatat tatgcctggc cgatggacca
      241 ctcaacttgt caataagcac ttaggttaca gatacacagg agtattcaaa accctggcaa
      301 gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc attggtacaa actttagttc
      361 gcgacaatgt aaagctaaat aatgatgtgt ataaagaact gaaaaagttt atgcacgatt
      421 acgacaaaac atccccagaa atgagaaaat accttaaa
//
LOCUS       DAZFZC010000412          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFZC010000412 DAZFZC010000000
VERSION     DAZFZC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.428"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            177..401
                     /locus_tag="ACHKXP_005560"
     CDS             177..401
                     /locus_tag="ACHKXP_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7650305.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQRTCSDRFT"
BASE COUNT          176 a           94 c           73 g          114 t
ORIGIN      
        1 cgaaaatgat aataattatc atctacatga ggtttatcac gacatgtgtg tacgccatca
       61 aaccacgaga aataatcaat tattacgcag gtatcgtatt aattgatctg catcaaatta
      121 gcgtaaaagc aacttcagat aatacaaatc agcaacactg aatacggggc aacattatgt
      181 catcaaagaa cagaacccgc agaacaacaa cccgcaacat ccgatttcca aaccagataa
      241 ttgaacaaat taacatcgct cttgacctga aaggttcagg taatttttca gcgtgggtta
      301 ttgaagcctg cagaagaaga ttaattaatg aaaaatattc tcaatttgta cccaacaaag
      361 acaaacacga ccagagaacc tgttcagaca ggtttactta aacgacttat atatgacaca
      421 aaaagcgacc actaaagtcg ctttttctta tggtaac
//
LOCUS       DAZFZC010000413          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFZC010000413 DAZFZC010000000
VERSION     DAZFZC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.429"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>434
                     /locus_tag="ACHKXP_005561"
     CDS             <1..>434
                     /locus_tag="ACHKXP_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO7650306.1"
                     /translation="LSAIHHQIIENQLDTEKITISYEFILCQHLIANTEFSYLALPEN
                     YNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYD
                     QILNEKTAGIAIATASILLTKRYNVDTYTEINSYLGKLRGQQ"
BASE COUNT          165 a           77 c           75 g          117 t
ORIGIN      
        1 ttttatcggc tattcatcac cagatcattg aaaaccaact ggatacagaa aaaataacta
       61 ttagttatga atttatcctc tgtcaacatc ttatcgcaaa taccgagttc agctatttgg
      121 cactccctga aaattataac cgtttgtttt taccaaattc aaaaaaccaa acaaataatc
      181 gtttcaaaac gttaaacagc aaagctattg gcaggctact tgctgctggt ggcgtataca
      241 atgggaacat tgaaggattc agagatactg cggaaaaact gggtggagat gcaataaaag
      301 gctatgatca aatactaaat gaaaaaacag cgggcatagc gatagcaaca gcatctattc
      361 ttttaacaaa gcgttataat gttgatacat atacagaaat aaatagttac ttaggcaaac
      421 ttagaggtca acaa
//
LOCUS       DAZFZC010000414          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFZC010000414 DAZFZC010000000
VERSION     DAZFZC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.430"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>428)
                     /locus_tag="ACHKXP_005562"
     CDS             complement(<1..>428)
                     /locus_tag="ACHKXP_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7650307.1"
                     /translation="CISLISQDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQ
                     NRIQFFELWLNSKLRHGNTVVLKIRNPRGQIKELRILDWNRVEPLVADDGDVFYRITP
                     DRNCGITESVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLA"
BASE COUNT           97 a          130 c          112 g           89 t
ORIGIN      
        1 gccagtcctg cggcatacac cggcggcagc cccacaagcg gatgaaaaaa acagttaaaa
       61 cggtcgtgga tcacctcccg ggcaggcacc gtcaccgact ctgtgatccc acagttccgg
      121 tccggcgtga tgcggtagaa cacgtcgccg tcatccgcca ccagaggttc aacccggttc
      181 cagtccagaa tacgcagttc tttgatctgc ccccgggggt tgcggatttt cagcaccacc
      241 gtattgccgt gacgcaattt ggaattcagc cacagttcaa aaaactggat gcgattctgc
      301 tgcgcattag gacgacgaca gagacgggca gtatctccct gccgcttttc ccggcgtatt
      361 ccctgtacat cagtctgcat caggcgaagc cgcattttgg cgatatcctg ggatatcagc
      421 gaaatgca
//
LOCUS       DAZFZC010000415          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFZC010000415 DAZFZC010000000
VERSION     DAZFZC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.431"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..355
                     /locus_tag="ACHKXP_005563"
     CDS             <1..355
                     /locus_tag="ACHKXP_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EspF repeat-containing protein"
                     /protein_id="HFO7650308.1"
                     /translation="RSLPPIAQALKDHLAAYEKSKSLDTSSLKPSRPAPPPPTSGLVS
                     GSSRSLPPIAQALKDHLAAYEKSKSLDTSGLKPSRPAPPPPTSGQVSEASRPLPPIAQ
                     ALKDHLAAYELSKKA"
BASE COUNT          123 a          106 c           93 g          104 t
ORIGIN      
        1 ccggtcttta ccgcccattg ctcaggcatt aaaagatcat ttggctgcct atgaaaagtc
       61 gaaaagtctt gatacttcaa gccttaagcc aagccgcccg gcaccgccgc cgccgacaag
      121 cgggttggta tctgggtcat cccggtcttt accgcccatt gctcaggcgc taaaagatca
      181 tttggctgcc tatgaaaaat cgaaaagtct tgatacttca ggtcttaagc caagccgccc
      241 ggcaccgccg ccaccgacaa gtggacaggt atccgaggca tcccggccat taccgcctat
      301 tgctcaggca ttaaaagatc atttagctgc ctatgaactg tcgaaaaagg cataaaaagt
      361 agatttaaaa ttattaatta ctgttgattg tttagagagt taaaagataa aaaccgatat
      421 taaggt
//
LOCUS       DAZFZC010000416          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFZC010000416 DAZFZC010000000
VERSION     DAZFZC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.432"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..176
                     /locus_tag="ACHKXP_005564"
     CDS             6..176
                     /locus_tag="ACHKXP_005564"
                     /inference="COORDINATES: protein motif:HMM:NF013681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008107 - galactoside
                     2-alpha-L-fucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HFO7650309.1"
                     /translation="MSRYANGIIANSTFSFWSAILGDNGDKKIICPIKWFADERLQKE
                     SFKIKPPHWIER"
     gene            173..>425
                     /locus_tag="ACHKXP_005565"
     CDS             173..>425
                     /locus_tag="ACHKXP_005565"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7650310.1"
                     /translation="MKKVSVLIASYNCAQFIGDTLHSLCSQTMNKDDYEIIIVDDGST
                     DDSVNIINSLIKEQKNVIFRSRTTNLGVIKTRNELLLLAK"
BASE COUNT          150 a           58 c           79 g          138 t
ORIGIN      
        1 atttaatgtc aagatatgct aatggtatta ttgcgaatag cacatttagt ttttggtctg
       61 caattttagg tgataatggg gataaaaaaa tcatatgccc tataaaatgg ttcgctgatg
      121 aaaggttgca aaaagaatca ttcaaaatca aaccacccca ttggatcgaa agatgaagaa
      181 agtttcagtg ttgattgcca gttataattg tgctcaattt attggtgata ctttgcattc
      241 tttatgttct cagactatga ataaggatga ttatgaaata attatcgttg atgatggctc
      301 aacagacgat agtgtaaata taattaactc tttgattaaa gaacagaaaa atgttatttt
      361 tcgctcccga actactaatc taggtgtaat taaaacaaga aacgagttac tcctgctggc
      421 aaaag
//
LOCUS       DAZFZC010000417          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFZC010000417 DAZFZC010000000
VERSION     DAZFZC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.433"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>425
                     /locus_tag="ACHKXP_005566"
     CDS             <1..>425
                     /locus_tag="ACHKXP_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7650311.1"
                     /translation="ALDVRLPEQFSIIGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPE
                     KPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSCQ
                     QPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTY"
BASE COUNT          104 a          110 c          122 g           89 t
ORIGIN      
        1 gcgctcttga tgtcagactc cctgaacagt tctcgataat cgggaaactc agagcgcatt
       61 atcctgtggt cacactctgc catgtgttcg gggttcatcg cagcagctac agatactgga
      121 aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt acgcagtcag gtacttgagc
      181 tacatggcat cagccacggt tcggccggag caagaagcat cgccacaatg gcaacccgga
      241 gaggctacca gatgggacgc tggcttgctg gcaggctcat gaaagagctg gggctggtca
      301 gctgtcagca gccgactcac cggtataaac gtggtggtca tgaacatgtt gctatcccta
      361 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
      421 cctat
//
LOCUS       DAZFZC010000418          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFZC010000418 DAZFZC010000000
VERSION     DAZFZC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.434"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..169
                     /locus_tag="ACHKXP_005567"
     CDS             <1..169
                     /locus_tag="ACHKXP_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650312.1"
                     /translation="EPTSDSSTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVI
                     TGVNYGTASGG"
     gene            214..>425
                     /locus_tag="ACHKXP_005568"
     CDS             214..>425
                     /locus_tag="ACHKXP_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650313.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQ"
BASE COUNT           97 a          111 c          149 g           68 t
ORIGIN      
        1 tgaaccgacc agcgacagca gcacaccgtc accggtggaa ctggtctcca tgtttcagac
       61 cggcagcgtg gccatccgtg cggagcgctg gatcaactgg cgtcgtcgcc gtaccgcggc
      121 agtggcggtg atcaccggtg tgaactacgg aactgcgtcc ggtggctgag tctgatgagg
      181 agggcgggag gcgcgagctt cccgcagtaa cagatggcaa aaatccagta tctgcagggc
      241 acgcatgatg cccgggccgg ggatatccgt gatgtggcac agccgtgtgc ggaggtgctg
      301 gttcgcctgg ggaaggcgga gtacatcaca gcgcgacgtc cggcaggtca gaaaaagaaa
      361 cgtgatgcgg agcatggcga atgtggaacc ttttgcggcg aacccgaaaa aaccagaaat
      421 cagga
//
LOCUS       DAZFZC010000419          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFZC010000419 DAZFZC010000000
VERSION     DAZFZC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.435"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..>424
                     /gene="grlR"
                     /locus_tag="ACHKXP_005569"
     CDS             134..>424
                     /gene="grlR"
                     /locus_tag="ACHKXP_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO7650314.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCYVRGNEK"
BASE COUNT          146 a           53 c           94 g          131 t
ORIGIN      
        1 gttggaatat gagtatattt aatgatatat tacattgcaa tctggagaaa aagaaaggtc
       61 tccgttattc ttgatatcgc ttatggatag aacaaattga aaggagtgag gttagtatga
      121 aactgagtga gttatgatta tgaaggatgg catctatagc attatcttta ttagcaatga
      181 agattcctgt ggggaaggta tactgattaa aaatggaaat ttgatcactg gcggagatat
      241 tgcttctgtg tatcaggggg tcctgtctga agaggaggac atcatacttc atgtccatcg
      301 ctataatcat gaaattccct cggtgctaaa cattgaacaa gattatcaat tagttatccc
      361 taaaaaagta ctgagtaatg atagtaatgt cacattacat tgctatgtaa gaggaaatga
      421 aaaa
//
LOCUS       DAZFZC010000420          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFZC010000420 DAZFZC010000000
VERSION     DAZFZC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.436"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>422
                     /gene="espL"
                     /locus_tag="ACHKXP_005570"
     CDS             <1..>422
                     /gene="espL"
                     /locus_tag="ACHKXP_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HFO7650315.1"
                     /translation="VKLARKKTCEHLLRARRKSKKLKTESRKKIARKIMAMRERIRKN
                     NKIKLEKEVNKSIKWVKDIQDIELVLMQDIMNKVHSSLTNALHSLDTSSRINWDDLLN
                     EVVRETLSHNNIVGAIKITKNPDIKLDPGEANNIQLIN"
BASE COUNT          181 a           56 c           79 g          106 t
ORIGIN      
        1 tagtcaaatt ggctcgaaaa aaaacatgtg aacatttact gagggcgagg aggaaatcga
       61 aaaaattaaa gacagaatcc agaaaaaaaa tcgccagaaa aataatggca atgagagaaa
      121 ggattcgaaa aaataataaa ataaaattag agaaagaagt taacaaatcg ataaaatggg
      181 ttaaagatat tcaggatatc gagttggtct taatgcagga cataatgaat aaggttcatt
      241 cgtcactgac taatgcatta cattctttag acacatcatc aagaattaat tgggatgatt
      301 tacttaatga agtcgtaagg gaaacgcttt cccataacaa tattgttgga gctattaaga
      361 tcactaaaaa cccagacatt aaacttgatc ctggagaggc taataatata cagcttatta
      421 at
//
LOCUS       DAZFZC010000421          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFZC010000421 DAZFZC010000000
VERSION     DAZFZC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.437"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>421
                     /locus_tag="ACHKXP_005571"
     CDS             <1..>421
                     /locus_tag="ACHKXP_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7650316.1"
                     /translation="LPAVEWLTYGSGWLAGMKLGDTPLVDFTRDRLHRETLRSFGRYE
                     LTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHT
                     TAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNR"
BASE COUNT           90 a          156 c          116 g           59 t
ORIGIN      
        1 gcctgcccgc cgtggaatgg ctgacctacg gcagcggctg gctggcaggc atgaagctcg
       61 gcgacacacc gctggtggat ttcacccgcg accgcctgca ccgggaaacg ctgcgcagct
      121 tcggtcgtta tgaactcacc accgcttaca cccctgccgg gcagttacag cgtcagcacc
      181 tgaacagcct gcagtatgac cgcgattaca cctggaacga caacggcgaa ctcatccgca
      241 tcagcagccc gcgccagacc cggagttaca gctacagcac caccggcagg ctgaccggcg
      301 ttcacaccac cgcagcgaat ctggatatcc gcatcccgta tgccacagac ccggcaggta
      361 accgcctgcc cgacccggag ctgcacccgg acagcaccct cagcatgtgg ccggataacc
      421 g
//
LOCUS       DAZFZC010000422          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFZC010000422 DAZFZC010000000
VERSION     DAZFZC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.438"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..>421
                     /locus_tag="ACHKXP_005572"
     CDS             63..>421
                     /locus_tag="ACHKXP_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650317.1"
                     /translation="MKTIDNQIRNISSSHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLK"
BASE COUNT          159 a           68 c           65 g          129 t
ORIGIN      
        1 tgtttttcag acaagtatca tttattttgt tacaaacaat tcaaaaatat cacaggttat
       61 ttatgaaaac aattgacaat cagattagaa acatctcttc aagccatcaa gataagcatt
      121 cagataaagt taacagtcat caacaccatg gcaaggttga taaaacacat cgtgcaaaga
      181 tagttgaatt tgataaactg gataatgatt cgcaaataga taacgatttt ggcctgcata
      241 tcatctattt cctgcaacat ggtcactgga aggtaaatga tcgtagtcac cagatggaga
      301 aagtatggtt ttataatagt gaaccttcta tagatattca agaatataat agattcgccg
      361 acaatactac tgatacgttt atctttacaa ttataccaga caataaccat gtgttaaaat
      421 t
//
LOCUS       DAZFZC010000423          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFZC010000423 DAZFZC010000000
VERSION     DAZFZC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.439"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..217
                     /locus_tag="ACHKXP_005573"
     CDS             <1..217
                     /locus_tag="ACHKXP_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO7650318.1"
                     /translation="IASRGNVADTGITDRVNDIINDRFWSDGKKPDRCDVLQELIDCG
                     DISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            238..>420
                     /locus_tag="ACHKXP_005574"
     CDS             238..>420
                     /locus_tag="ACHKXP_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO7650319.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIE"
BASE COUNT          152 a           64 c           97 g          107 t
ORIGIN      
        1 gatagcttct cgtggaaatg ttgcagacac agggataact gatcgtgtca atgacataat
       61 aaatgacaga ttctggagtg atgggaaaaa acccgacaga tgtgacgtac ttcaggaact
      121 aattgattgt ggtgatatta gtgctaaaga tgcaaaaagc acacagaaag cctggaattg
      181 tcgtcactcc agacagtcaa acgataaaaa aagatagccc ttgtggaggt tcctgcaatg
      241 tcaaatacat accagaaaag aaaggcaagt aaagagtatg gtttatataa tcaatgtaag
      301 aaactaaatg atgatgaatt atttcgctta cttgatgatc acaattcctt gaaaaggatt
      361 tcatctgcca gagtattaca gttaagaggt gggcaagacg ctgttagatt ggcaattgag
//
LOCUS       DAZFZC010000424          418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFZC010000424 DAZFZC010000000
VERSION     DAZFZC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.440"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..272)
                     /locus_tag="ACHKXP_005575"
     CDS             complement(<1..272)
                     /locus_tag="ACHKXP_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7650320.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLR"
     gene            complement(328..>418)
                     /locus_tag="ACHKXP_005576"
     CDS             complement(328..>418)
                     /locus_tag="ACHKXP_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7650321.1"
                     /translation="RYEDVLWPEAASDETKKRTAFAGTAISIV"
BASE COUNT          109 a          107 c          112 g           90 t
ORIGIN      
        1 cgcagggtca tctgcggatt cacctcatgc ttcggcttga catatcccgg tgtaaattca
       61 gaggtggagc cgccacggga gcggataacc tcaccggaaa caatcggcga aacgtacagc
      121 gccatgttta ccagtcccgg aatttgtgag agatagactt tctccgtggt gaagggatag
      181 ctctcacgga aaaagagacg cagaaacagc ggatcaaact taaatttctg ctcatttgcc
      241 gccagcagct gggcggttgt gtacatcgac ataaaaaaat cccgtaaaaa aagccgcaca
      301 ggcggccttt agtgatgaag ggtaaggtta aacgatgctg attgccgttc cggcaaacgc
      361 ggtccgtttt ttcgtctcgt cgctggcagc ctccggccag agcacatcct cataacgg
//
LOCUS       DAZFZC010000425          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFZC010000425 DAZFZC010000000
VERSION     DAZFZC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.441"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>415
                     /locus_tag="ACHKXP_005577"
     CDS             <1..>415
                     /locus_tag="ACHKXP_005577"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO7650322.1"
                     /translation="VKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGENHPLNR
                     FTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGD
                     GAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNV"
BASE COUNT          124 a           66 c           88 g          137 t
ORIGIN      
        1 gtgaaaaatt tctccagtgt cggcgctttt tcatttccga cctgccattt ttcagggaat
       61 atacgtatag gtcgattttg tagtattgca tcaaatgtaa aaataatggg tgaaaatcat
      121 cctttgaatc gttttactac tcacatgatg acttataacg gtgaatttga taagtttgct
      181 atgagtgaat ttgaacgtag ctggacattg aaaccattca ttaccaaacc tgaaaatccg
      241 attataggaa atgatgtctg gataggaaat gatgttgtat tgaaaggtgg catcgcaata
      301 ggtgatggtg ctgttattgc tgccaattcc gttgttacca aagatgtgcc cccatatgcc
      361 atagttgcag gagttcctgc aaaaataatc agatttaggt ttgattcaaa tgtta
//
LOCUS       DAZFZC010000426          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFZC010000426 DAZFZC010000000
VERSION     DAZFZC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.442"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>412
                     /locus_tag="ACHKXP_005578"
     CDS             <1..>412
                     /locus_tag="ACHKXP_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7650323.1"
                     /translation="NPYGTSKYFIERILEDYCKSNKSFSAISLRYFNPTGAHKSAKIG
                     EDPNGIPNNLIPYISHVASGKLDYLNVYGNDYDTIDGTGMRDYIHVVDLADGHIKALE
                     YMLTNKIGYDIFNLGTGKAYSVLQVIQAFEKISM"
BASE COUNT          144 a           64 c           75 g          129 t
ORIGIN      
        1 caaatcctta tggaacttct aagtatttta ttgaaagaat ccttgaagat tattgtaagt
       61 ctaataaatc attttcagca ataagcctcc gctattttaa cccaacaggt gcgcacaaat
      121 cagctaagat cggtgaagat ccaaatggca ttcctaataa tctaatacca tatatatcac
      181 acgtggcttc tggaaaacta gattatctga atgtttatgg aaatgactat gatacgattg
      241 atggcacagg tatgcgtgat tatatccatg ttgttgattt ggctgacgga catataaaag
      301 ccttggagta tatgctaact aataaaatag ggtatgatat atttaattta ggcacaggca
      361 aagcatatag cgtattgcaa gttattcagg catttgaaaa aatttcaatg aa
//
LOCUS       DAZFZC010000427          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFZC010000427 DAZFZC010000000
VERSION     DAZFZC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.443"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          116 a           96 c          102 g           95 t
ORIGIN      
        1 acagcctgcg gccatttttg tctttatcac ttccacaagt aaatattggc aacccgagct
       61 ggaagcctca tccagagcac tcatgaaatg actgtaacag ctgttgaaaa accggaatgc
      121 actcttcatt gcaaacattt cagggatgaa gtgaagatga attgagtcgt agtatccaca
      181 ccaagccggc gttatccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
      241 gttgctggaa agagaaaacc cccgcacgtt gagaggtaca acctgacaac agaacggggg
      301 ctaatcttga accagaacaa ttcaagaata gccgcaaaat gttgtcatta caacaaggcg
      361 gctatatgac gctcgcgcag tttgccatga ttttctggga cgacctggc
//
LOCUS       DAZFZC010000428          408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFZC010000428 DAZFZC010000000
VERSION     DAZFZC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.444"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>408
                     /locus_tag="ACHKXP_005579"
     CDS             <1..>408
                     /locus_tag="ACHKXP_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7650324.1"
                     /translation="SLTHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDA
                     PVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVF
                     TAGSATVTPKLNGVAGPAFQVIKDSLTLGLNAL"
BASE COUNT           92 a          105 c          125 g           86 t
ORIGIN      
        1 ggtccctgac gcatccggtg gatgacgcaa ttaccctgct gacacagggc ggcagactga
       61 cctgtaagtt ccgcctgtca ggcgcactga ccaacaatca gttcgggctg gggatttatc
      121 tgtatacgga tgctcccgtt cctgatggtg tggcgatgac gggtaccggt aatccgttcc
      181 tgatgtcgta cttcactcag accactgacg gcagagtgaa tctgatgcat cacaggaaag
      241 ccggaaacac gaagctgggg gagttcggcg attacggtaa cgactggcag acgctggagc
      301 tggtgttcac cgccggcagt gccacggtta ctccgaaact gaatggagtg gctggcccgg
      361 cattccaggt tataaaagac agtctgacac tgggactgaa tgcgctga
//
LOCUS       DAZFZC010000429          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFZC010000429 DAZFZC010000000
VERSION     DAZFZC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.445"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..78
                     /locus_tag="ACHKXP_005580"
     CDS             <1..78
                     /locus_tag="ACHKXP_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7650325.1"
                     /translation="RRISQDISTRDEGGDGKMVVIGRQA"
BASE COUNT          123 a           77 c          116 g           88 t
ORIGIN      
        1 cggcgaatct cccaggacat cagtacccgt gatgaaggcg gtgacgggaa gatggtggtt
       61 atcgggcggc aggcataaaa gcgaaaaaat cccgcagtgc tcacggacag gaactgcggg
      121 agcgttacga agattgagtg taaggaatta ttcttatgtc acgacaaaaa acattaacgc
      181 agagaaatta ttagcgccac agtcagtttg tgaaaatgtg aagatattca gaatttttat
      241 tcagtcatga tacaggcatc ctccgggatg cctgttgttt ttgtgcgtaa cagttatcac
      301 agtaaagggt gagacaatgg gcaaaggtgg cggcaaggcg cacacgccgg tagaggcaaa
      361 ggacaatctt aagtccacgc agatgatgag cgtgattgat gcca
//
LOCUS       DAZFZC010000430          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFZC010000430 DAZFZC010000000
VERSION     DAZFZC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.446"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>399)
                     /locus_tag="ACHKXP_005581"
     CDS             complement(<1..>399)
                     /locus_tag="ACHKXP_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7650326.1"
                     /translation="QTLTFVQDRPSDVVWPYTNCDVVVDDNGVGFRYSFSALKDRHTA
                     VEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGL
                     LETQTVDFTLGSQGLRHTPGDIIEICDNDYA"
BASE COUNT           73 a          132 c          110 g           84 t
ORIGIN      
        1 ggcatagtcg ttatcacaga tttcaataat gtcaccgggt gtgtgacgca gcccctgtga
       61 cccgagcgtg aaatccaccg tctgcgtttc cagcagtccg gtctttatca cccacagccc
      121 ggcacggtgg gcctgaccgc gactggtgca accgaacgca tccatcttca gcaggttgcg
      181 cccgtagcgc agtatggctt ccgggtcttc caccagttcc gtggaggtct gccagccgtt
      241 ctgcgggtcg gtgtaattca cctccaccgc cgtgtggcgg tccttcaggg cgctgaagct
      301 gtagcgaaac cccacgccgt tatcatccac caccacatcg cagttggtgt acggccacac
      361 cacatccgac gggcggtcct gaacgaacgt cagcgtctg
//
LOCUS       DAZFZC010000431          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFZC010000431 DAZFZC010000000
VERSION     DAZFZC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.447"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>398
                     /locus_tag="ACHKXP_005582"
     CDS             <1..>398
                     /locus_tag="ACHKXP_005582"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFO7650327.1"
                     /translation="SDGKVYFLGSSELVLEKIKCNMYNDFPNILIKTLSPPFKSTFSV
                     NEINDFVEDINKFSPDAVFVGLTAPKQEKLIHEIKKRINTKLISGVGAVFDFYAGTIT
                     RPSQLWIDLHLEWLIRFLGEPKRLWRRNFI"
BASE COUNT          135 a           63 c           73 g          127 t
ORIGIN      
        1 tgtcggatgg aaaagtctat tttcttggtt cttcagagtt agttctggag aaaataaaat
       61 gcaacatgta taatgatttt cccaacatat taattaaaac attatcacca ccatttaaat
      121 caaccttctc agtcaatgaa ataaatgatt ttgttgaaga tatcaacaaa tttagtccag
      181 atgccgtttt tgtcggatta accgcaccta agcaggaaaa gttaatacat gaaataaaaa
      241 aaagaatcaa tacgaaatta atatcaggtg ttggtgcggt cttcgatttt tatgccggta
      301 ctataacacg tccatcacaa ttatggattg atttgcattt agagtggctt atacggttct
      361 tgggggaacc taaacggcta tggcgtagga atttcata
//
LOCUS       DAZFZC010000432          392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFZC010000432 DAZFZC010000000
VERSION     DAZFZC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.448"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(76..285)
                     /gene="fumD"
                     /locus_tag="ACHKXP_005583"
     CDS             complement(76..285)
                     /gene="fumD"
                     /locus_tag="ACHKXP_005583"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO7650328.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          115 a           97 c           59 g          121 t
ORIGIN      
        1 ttatttagaa tgattaactg gaccgcaact gaagcaagaa tatcaggatg aagcttaccg
       61 cctcatcctg aatggtcatt ttaccagtgc gttaatgacg gtttccatcg cctgcttttc
      121 cagctcactg cgctgaatgc gtttattggc taatgctgta cgtaatacgc ctgcgataac
      181 aatatgcttt ggttcctgcc ccagatcgcg catttccagc acaactttac cgacaactct
      241 gcacatttct cggtacagtt catcctcttt tgtccgattt cccattcttt atacctattt
      301 atcatgccaa ctatcagcat atatcaatct aaccaattaa acaaaaatca aacaaaatca
      361 gacaaataac gcgataaatt attttaactg ta
//
LOCUS       DAZFZC010000433          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFZC010000433 DAZFZC010000000
VERSION     DAZFZC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.449"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..116
                     /locus_tag="ACHKXP_005584"
     CDS             3..116
                     /locus_tag="ACHKXP_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO7650329.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            185..>385
                     /gene="ydfK"
                     /locus_tag="ACHKXP_005585"
     CDS             185..>385
                     /gene="ydfK"
                     /locus_tag="ACHKXP_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO7650330.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVL"
BASE COUNT          150 a           72 c           62 g          101 t
ORIGIN      
        1 atatgaacag catactgata atcacttctc tccttatcat attcagcatt tttagtcatg
       61 ccctaataaa attagggatt ggcatatcca ataacccaga caaaaccgat gtataagtca
      121 acatatcctg aatcagacat acaatatcgc aatgaaaatc aataatattt taaggaatat
      181 cttcatgaaa tcaaaagaca ccctaaagtg gttccctgcg cagcttcctg aagtaagaat
      241 tatcctaggg gatgctgtag tggaagtagc aaaacaggga agacctatca ataccagaac
      301 attgcttgat tacattgaag gaaacataaa gaaaaaatca tggctggata acaaagaatt
      361 attacaaaca gcgatatcag ttctt
//
LOCUS       DAZFZC010000434          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFZC010000434 DAZFZC010000000
VERSION     DAZFZC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.450"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..>385)
                     /locus_tag="ACHKXP_005586"
     CDS             complement(59..>385)
                     /locus_tag="ACHKXP_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7650331.1"
                     /translation="FRGRARRKEYWMFILVNIIFTFVLGLLDKMLGWQRAGGEGILTT
                     IYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFNCQAGTPGENRFGP
                     DPKLEP"
BASE COUNT          106 a          105 c           97 g           77 t
ORIGIN      
        1 attcattaac atgatgataa aagccggatg agtcagacat ccggcgagca gatttgtttt
       61 aaggttccag ttttggatct ggtccaaagc ggttctcgcc tggcgtaccc gcctggcagt
      121 taaagacgat gataataagc cagccgataa acgggattaa gaatagcagt gcccaccacg
      181 ccgagcggtc ggtgtcgtgc aggcggcgga actgaaccgc ccaccacggt aaaaacacca
      241 gaataccgta gattgtcgtc aggatgcctt cgccaccagc gcgttgccag cctaacattt
      301 tatccagcag ccccagcacg aacgtaaaga tgatgttgac cagaataaac atccagtact
      361 ctttgcgccg cgcacgaccc cggaa
//
LOCUS       DAZFZC010000435          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFZC010000435 DAZFZC010000000
VERSION     DAZFZC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.451"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>383
                     /locus_tag="ACHKXP_005587"
     CDS             <1..>383
                     /locus_tag="ACHKXP_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rhs family protein"
                     /protein_id="HFO7650332.1"
                     /translation="DASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHL
                     VFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQT
                     SLWGLALSLPADTGYPDPRGELDPEA"
BASE COUNT           87 a          112 c          128 g           56 t
ORIGIN      
        1 gacgccagcg gcagacgaac agagaaacgc tgcgaccgga agaaaatccg cttcacgtac
       61 ctgtgggacg gcgacagtat tgcggaaatc cgggaatacc gcgatgataa actgtacagc
      121 gtacggcacc tggtgtttaa cggctttgag ctgataagcc agcagttcag ccgggtacgg
      181 caggcgcatc cgtcggtggc cccgcagtgg gtgacgcgga cgaatcatgc ggtgagcgac
      241 ctgacgggcc gcccgctgat gctctttaac agtgaaggta aaaccgtctg gcgaccggga
      301 cagaccagcc tgtgggggct ggcactcagc ctgcccgcag acaccggcta cccggacccg
      361 cgcggggaac tggacccgga agc
//
LOCUS       DAZFZC010000436          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFZC010000436 DAZFZC010000000
VERSION     DAZFZC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.452"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(90..>383)
                     /gene="rayT"
                     /locus_tag="ACHKXP_005588"
     CDS             complement(90..>383)
                     /gene="rayT"
                     /locus_tag="ACHKXP_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO7650333.1"
                     /translation="SDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHAIRNTKDYKHH
                     VDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDLSAGERIIL"
BASE COUNT          105 a          102 c           79 g           97 t
ORIGIN      
        1 gcgtaaacgc cttatccggc ctaccgatcc ggcacaggtt tgtaggcatg ataagacgcg
       61 ccagcgtcgc atcaggcacc agcgcacagt tataaaatga tacgctcccc ggcacttaaa
      121 tccgttacgt cccccgccca atcgatggga tataaccctc gcgcgacatc gcgatggaac
      181 gttgagaatg gccaatcact cacttgcttt acccaaccat gttttactgg atttatataa
      241 atataatcaa catgatgctt ataatcctta gtattgcgga tggcgtgttc ccaaaaacgc
      301 ggttgccaga tatttttcaa tccacaagca tgggtaaatt gctttttaat ttcccgccag
      361 cgagaggaaa aatcatcatc gct
//
LOCUS       DAZFZC010000437          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFZC010000437 DAZFZC010000000
VERSION     DAZFZC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.453"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>382
                     /locus_tag="ACHKXP_005589"
     CDS             <1..>382
                     /locus_tag="ACHKXP_005589"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7650334.1"
                     /translation="IYIGYLESQFDITDIIKYVRDSKKYELTIVGHGAKNEYYKDIAS
                     NSTSIHFKGLLPSDEVVNVMSDSHIGVLPITRTAQMPNKLFDYVGGCIPILSIGASDS
                     SEFVKENDIGWNSDWGTNNIAQVL"
BASE COUNT          128 a           56 c           73 g          125 t
ORIGIN      
        1 taatatatat aggatatttg gaatctcaat ttgatataac cgatattata aaatacgtcc
       61 gagatagtaa gaaatacgaa cttacgatag tcggtcatgg ggccaaaaat gaatattaca
      121 aagatatcgc ctcgaattct acttctatcc attttaaagg acttcttcct tccgatgaag
      181 ttgttaatgt tatgtctgat tcacatatag gtgttttgcc tataaccaga actgcgcaaa
      241 tgcctaataa actatttgat tatgtaggcg gttgtatacc tattttaagt atcggtgcat
      301 cagattcctc agagtttgta aaggaaaatg atattggatg gaatagtgac tggggaacta
      361 ataatatagc tcaggtatta ga
//
LOCUS       DAZFZC010000438          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFZC010000438 DAZFZC010000000
VERSION     DAZFZC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.454"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..123)
                     /locus_tag="ACHKXP_005590"
     CDS             complement(<1..123)
                     /locus_tag="ACHKXP_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFO7650335.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKN"
     gene            complement(120..>380)
                     /locus_tag="ACHKXP_005591"
     CDS             complement(120..>380)
                     /locus_tag="ACHKXP_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7650336.1"
                     /translation="EWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQPTSRM
                     RVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
BASE COUNT           86 a          119 c           78 g           97 t
ORIGIN      
        1 gtttttgtcg ctcgctgccg aatccagcca gaatccaaac tcaatatcca gtgcatcaaa
       61 aagccaaagc ggcgtttgcc agcagtcctt gtcgtgtgct ggcgtatttg atttgatagt
      121 catgcagccc gatctcccca tcgcgctttc cattcgagag ccagtcgcgc ttcgtctgac
      181 cacttaacgc cacgctctgt accgaatgcc tgtataagct ctaatagctc cgcaaattcg
      241 cttacacgca tcctgctggt tggctggcct attaccacaa agccattccc ggcaaggtta
      301 ggaacaacat cctgctgctt taatgctgcg gtaaacacac acttccagct ttctgcatcc
      361 agccagcgac catgccattc
//
LOCUS       DAZFZC010000439          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFZC010000439 DAZFZC010000000
VERSION     DAZFZC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.455"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..324)
                     /locus_tag="ACHKXP_005592"
     CDS             complement(<1..324)
                     /locus_tag="ACHKXP_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7650337.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTI"
BASE COUNT           85 a          139 c           80 g           76 t
ORIGIN      
        1 tatcgtgcaa ctgaaatcac tgtaccgggc attatccgtg acactccact cacggcacac
       61 aaccctcacc gtccggttat gtttcggcgg tcgccacaaa aaggcacggt aaccaccatg
      121 ccacgataaa aactcttcca gccagcgccg ggttgactca tccgtcaccc ggaacaccgc
      181 ctgaaacgtc ttcagttgag gattcagccc tgtggggcgg cgctgttcat aaccgtcacc
      241 aaaccgcacc ctcaccaccg acggcttctc actcacctgc atcccttcac gcgggaccag
      301 atgcagcgtt tttatctcag ccactcagca ttcctccgtc acgtcgcatg gacagcatca
      361 ccgcctgcac ccgctggtca
//
LOCUS       DAZFZC010000440          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFZC010000440 DAZFZC010000000
VERSION     DAZFZC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.456"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>378
                     /locus_tag="ACHKXP_005593"
     CDS             <1..>378
                     /locus_tag="ACHKXP_005593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650338.1"
                     /translation="SMNVIIKNNLTFDTTLGLGTFYPCCEENKFIIEYYKYAVVEFLE
                     LTPVLHTTKREHRLSIGEGHYRSRGTIAKEFNFLVRIALVLRWSLRENNNVSFQKRIT
                     YLWQGIQAQKKTHIISPYESPAEG"
BASE COUNT          133 a           57 c           70 g          118 t
ORIGIN      
        1 agcatgaatg taattatcaa aaataactta acttttgata caacattggg gttagggaca
       61 ttttatcctt gctgtgaaga aaataaattt attattgaat attataaata tgcagttgtt
      121 gaatttttgg aacttacacc ggtacttcat actacaaaaa gagagcatag attatctata
      181 ggggaagggc attatcgaag tcgaggaaca attgctaaag aattcaactt tcttgtgcgc
      241 attgctttag tattacgttg gagtcttcga gaaaacaaca atgttagttt tcaaaagcga
      301 attacttact tatggcaagg aatacaggca caaaaaaaga ctcatatcat ctcaccttat
      361 gagtcccctg ctgaaggt
//
LOCUS       DAZFZC010000441          373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFZC010000441 DAZFZC010000000
VERSION     DAZFZC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.457"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..180)
                     /locus_tag="ACHKXP_005594"
     CDS             complement(<1..180)
                     /locus_tag="ACHKXP_005594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650339.1"
                     /translation="MKYRTKIKRKMPYAGCRYRVFIGKLCVIATLPSVIRKRRTGILI
                     NIISLGTGGYNQYDEL"
BASE COUNT          114 a           74 c           69 g          116 t
ORIGIN      
        1 caactcgtca tattgattat acccccccgt tcccagagaa ataatattta ttaaaattcc
       61 agttcttctt tttctgatta cagaaggcaa agtggcaatt acgcatagtt tcccgataaa
      121 gacgcgatag cgacatcccg cataaggcat ttttctcttt atctttgtac ggtacttcat
      181 ggaacagagt ttttgacctt gcgaatcgtg atgtctgttg gggagggaca atttgctcac
      241 tgaagcgtga gactcgatta agcgcacgaa acacagaaat caaaaaaacc ggtcactttt
      301 ttacaaggta accgggtaaa aataattttt attttttaac tgttttgaga ctcatagaga
      361 tgtctcaaaa cta
//
LOCUS       DAZFZC010000442          373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFZC010000442 DAZFZC010000000
VERSION     DAZFZC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.458"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>373
                     /locus_tag="ACHKXP_005595"
     CDS             <1..>373
                     /locus_tag="ACHKXP_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650340.1"
                     /translation="PESPTGNNLEALAPGIEKLKQTSIEMVTLLNTLQPGGKCIITGD
                     FQKELAYLQNVILYNVSSLRLDFLGYNAQIIQRSDNTCELTINEPLKNQEISTGNINI
                     NCPLKDIYNEIRRLNVIFSCGT"
BASE COUNT          136 a           68 c           59 g          110 t
ORIGIN      
        1 tcctgaaagc ccaacaggaa ataacctcga agcactagca ccaggtatag aaaaactaaa
       61 acagacctct attgaaatgg tcactttact taacacgtta caacctggtg gaaaatgcat
      121 tatcactggt gattttcaaa aagaattagc gtacttacaa aatgtaattc tttataatgt
      181 ctcgtctctt cgtctggatt ttttaggtta taacgcccaa attattcaac gatcggacaa
      241 tacttgtgaa cttaccatta atgaaccgtt aaaaaaccag gaaatatcca caggtaatat
      301 caatattaat tgcccattaa aagatattta caatgaaatc aggaggttaa acgtaatttt
      361 tagttgtggg act
//
LOCUS       DAZFZC010000443          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFZC010000443 DAZFZC010000000
VERSION     DAZFZC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.459"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..202
                     /locus_tag="ACHKXP_005596"
     tRNA            127..202
                     /locus_tag="ACHKXP_005596"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:161..163,aa:Glu,seq:ttc)
BASE COUNT          105 a           80 c           84 g           96 t
ORIGIN      
        1 aaaagtgaat agcaaggcgt ttacgcgttg ggagtgaggc tgaagagaat aaggccgttc
       61 gctttctatt aatgaaagct caccctacac gaaaatatca cgcaacgcgt gataagcaat
      121 tttcgtgtcc ccttcgtcta gaggcccagg acaccgccct ttcacggcgg taacaggggt
      181 tcgaatcccc taggggacgc cacttgctgg tttgtgagtg aaagtcgccg acctcaatat
      241 ctcaaaacag actgttaagt cttgtttgat atatttgctc tttaaaaatc tggatcaagc
      301 tgaaaattga aacactgaac aatgaaagtt gttcgtgagt ctctcaaatt ttcgcaactc
      361 tgaag
//
LOCUS       DAZFZC010000444          361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFZC010000444 DAZFZC010000000
VERSION     DAZFZC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.460"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..273
                     /locus_tag="ACHKXP_005597"
     CDS             <1..273
                     /locus_tag="ACHKXP_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO7650341.1"
                     /translation="EPSAEAWYLWQEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDV
                     LCDTDLQRVFTPDDREQVLAVYGPVHARLLRQALELIADAESARKK"
     gene            297..>361
                     /locus_tag="ACHKXP_005598"
     CDS             297..>361
                     /locus_tag="ACHKXP_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7650342.1"
                     /translation="MRLALRLGRTLSELRHSLSAS"
BASE COUNT           70 a           95 c          120 g           76 t
ORIGIN      
        1 gagccttcgg cagaggcctg gtatctgtgg caggaagtgc tcaatggtga tggagaggat
       61 gacgataccc tgtcggtggt ggcgaaaacc cgccgtaacc tggaagcgga tgtgacgctg
      121 ttctgcgatg tcctgtgtga tactgacctg caacgggtgt tcactccgga cgaccgtgag
      181 caggtgctgg ccgtctatgg tccggtacat gcccggttgc tgcgtcaggc actggaactg
      241 atcgctgatg cagagtcggc cagaaaaaag tagcccgccc ggaaattcgc tttctgatgc
      301 gacttgcgct ccgtctgggg cgcaccttat ccgaactgcg gcacagcctg agtgcgagcg
      361 a
//
LOCUS       DAZFZC010000445          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFZC010000445 DAZFZC010000000
VERSION     DAZFZC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.461"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>356
                     /locus_tag="ACHKXP_005599"
     rRNA            <1..>356
                     /locus_tag="ACHKXP_005599"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          108 a           81 c          112 g           55 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag
      121 tgtgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgt
//
LOCUS       DAZFZC010000446          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFZC010000446 DAZFZC010000000
VERSION     DAZFZC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.462"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..284
                     /locus_tag="ACHKXP_005600"
     CDS             <1..284
                     /locus_tag="ACHKXP_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7650343.1"
                     /translation="RELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPSVV
                     DPDLIWSLPDGEIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          104 a           71 c           90 g           83 t
ORIGIN      
        1 ccagagagct ggttgccagt ggcgatgtat acaactctgg ttacgggtta ttcccgtctg
       61 aacaggctcg taaggactgg caaaatgccc gcaaaaaact atctagggca aaggtgaaga
      121 aaccatctgt ggttgatccg gatcttatct ggtcattacc agacggagaa atacgccgct
      181 acgacaggcg tctgaacata atctgtagcg agtgccggaa gagcgaagct atgcagcgtg
      241 tactggcttt ctatcagggt aattttcaga aggtgctgtt gtgagccaaa ttaacaatcg
      301 gaacttcgtg aagagaaagc ataatccaaa tctgaataat taagttca
//
LOCUS       DAZFZC010000447          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFZC010000447 DAZFZC010000000
VERSION     DAZFZC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.463"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            108..326
                     /gene="ygeI"
                     /locus_tag="ACHKXP_005601"
     CDS             108..326
                     /gene="ygeI"
                     /locus_tag="ACHKXP_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO7650344.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
BASE COUNT          135 a           56 c           55 g          101 t
ORIGIN      
        1 ataaaaatat ctaagatatt caccttattg caagatattt aaaatgctct agagttaaaa
       61 gtatgatgta aagaccattg gcctggacaa ttaaaaaagg gagctttatg actaatccaa
      121 tcggtatcaa taatttatct caaagttcaa atatagcgaa tgcaacgggt gatgaagtcg
      181 tatcattaga taaacacatc aacaccagtg ctactgatac agatcaaata caagcattta
      241 tagtttcaac atggatggcg ccttttcaaa atgatatgta ttcagaagat aatcctatct
      301 caccttacta taaaattgag tggtagacga ttgaccaaat aaatcat
//
LOCUS       DAZFZC010000448          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFZC010000448 DAZFZC010000000
VERSION     DAZFZC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.464"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          120 a           57 c           81 g           83 t
ORIGIN      
        1 atcaactata gttgcagttg gacaaattcc aaatacaaat tctggagaca ttgataatat
       61 tggtgagtgt caaattttca tcaggtataa acacagctgg attgagaata aattcaaaga
      121 aaaaataatt gaataaccat gttatgtata gacattttag atggaacgaa ccagaggaag
      181 gcatgcctcc gacttgggtt atatgggcac ggatttttag tgtagtgaac tcaacgacgc
      241 aaaccgaata acctgccgct agtgcttatc agcacggaag gggcaataga atggtgcgca
      301 gaatacgatg agtggggcaa cctgccaggg aaagatcatc a
//
LOCUS       DAZFZC010000449          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFZC010000449 DAZFZC010000000
VERSION     DAZFZC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.465"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>335
                     /locus_tag="ACHKXP_005602"
     CDS             <1..>335
                     /locus_tag="ACHKXP_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFO7650345.1"
                     /translation="VSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKV
                     YFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKT
                     PKEIIERGVA"
BASE COUNT          112 a           74 c           74 g           75 t
ORIGIN      
        1 gtctcagtaa atcaggctct taccgacaaa ttcctgagtt taccgtcaga actcagaaaa
       61 tcactgacat gggacagagg aatggaactg gccagacatc tagaatttac tgtcagcacc
      121 ggcgttaaag tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaac
      181 acaaatgggc taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa
      241 catgaactag atctggttgc tgctcagcta aacaacagac cgagaaagac actgaagttc
      301 aaaacaccga aagagataat tgaaaggggt gttgc
//
LOCUS       DAZFZC010000450          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFZC010000450 DAZFZC010000000
VERSION     DAZFZC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.466"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>335
                     /locus_tag="ACHKXP_005603"
     CDS             <1..>335
                     /locus_tag="ACHKXP_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650346.1"
                     /translation="IRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGRR
                     VAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIR
                     VETATGELA"
BASE COUNT           81 a           97 c          105 g           52 t
ORIGIN      
        1 tatccgcacg gatgatgagc ggactcaccg gtaccattac gacagtcagc accggctggt
       61 gcactacacg cggacacaat atgaagagcc gctggtcgaa agtcgctatc tttacgaccc
      121 gctgggccgc agggtggcaa aacgggtgtg gcggcgtgaa cgggacctga cgggctggat
      181 gtcgctgtca cggaaaccgc aagtgacctg gtacggctgg gacggcgacc ggctgaccac
      241 aatacagaac gacagaaccc gcatccagac gatttatcag ccggggagct tcacgccact
      301 catcagagtt gaaaccgcca ccggtgagct ggcga
//
LOCUS       DAZFZC010000451          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFZC010000451 DAZFZC010000000
VERSION     DAZFZC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.467"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           86 a           95 c           79 g           72 t
ORIGIN      
        1 agttgatgct cgttacgccc gtaaaatacc gccttactgc tttaactgtt ccgcgtgcgc
       61 atagccgcat tcaccgcatc acaaaattca ctttaaaaag ggcggatatc cgtttccgcc
      121 gaatcaccag aaaagtgata acagagggcg ttgcagcggg gttgtcactt aagcgtatgg
      181 tcaacctgac aacccggtgt cctcagcggg gaaggaataa ccccgccata cttaccgccg
      241 cgccatttcg cggattgcca caaccggaag cgcacggtcg aattaaattt aacgactacc
      301 tacagagaga cgaacttcgc cgtgcgcttt cg
//
LOCUS       DAZFZC010000452          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFZC010000452 DAZFZC010000000
VERSION     DAZFZC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.468"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            268..>331
                     /locus_tag="ACHKXP_005604"
     CDS             268..>331
                     /locus_tag="ACHKXP_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFO7650347.1"
                     /translation="MHQMEKITTGVSYTTSAVGTG"
BASE COUNT           80 a           66 c           89 g           96 t
ORIGIN      
        1 ccactctgaa ggacgctggc taaaacgggt agatgtactt cacatgatac ttatatttgg
       61 cagtacattt tctgacagac agtgacggat gttgtcaaga tattgtgtca tttataacct
      121 gaatcagggg ttggtcggaa tgttatctgg catttttagc agagcctgaa tgccataatc
      181 acggctcccg gagttggccg tcagtgggtg acactggcgg ctttttgttt tcctttactt
      241 tcattttctg tcggcggtga cggagacata catcagatgg aaaaaatcac aacgggtgtg
      301 tcatacacca cgtcagcggt ggggacggga t
//
LOCUS       DAZFZC010000453          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFZC010000453 DAZFZC010000000
VERSION     DAZFZC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.469"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..331
                     /locus_tag="ACHKXP_005605"
                     /pseudo
     CDS             16..331
                     /locus_tag="ACHKXP_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT           73 a           98 c           82 g           78 t
ORIGIN      
        1 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
       61 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
      121 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
      181 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
      241 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
      301 tacagccggg cagtgacgtc atcgtctgcg c
//
LOCUS       DAZFZC010000454          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFZC010000454 DAZFZC010000000
VERSION     DAZFZC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.470"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..164)
                     /locus_tag="ACHKXP_005606"
     CDS             complement(<1..164)
                     /locus_tag="ACHKXP_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7650348.1"
                     /translation="MIQAEQENDMNILRKLMERLCGCGKHDGREHVQSLTAQLRLGPA
                     DILESDENGI"
     gene            complement(139..>324)
                     /locus_tag="ACHKXP_005607"
     CDS             complement(139..>324)
                     /locus_tag="ACHKXP_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7650349.1"
                     /translation="SNGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQ
                     DVKGLDEVGNDTGRTGE"
BASE COUNT           75 a          102 c           67 g           80 t
ORIGIN      
        1 ataataccat tctcatcgga ctccaggatg tctgccggcc ccagacgcag ttgtgctgta
       61 agcgactgca cgtgttcacg gccatcatgc tttccgcaac cacacagacg ctccataagc
      121 tttctcaaaa tattcatgtc attctcctgt tctgcctgta tcattgccca cttcatccag
      181 tcccttaaca tcctgccacg gcccgtcgcc aaacctgacc tgcaaatgct gaaaaaaacc
      241 ctgaacccgt gtggcatctt tggggtcaag aaaggtcagt ccggtgatga gtgcgccatc
      301 tgtatccggg aaccagccat tgct
//
LOCUS       DAZFZC010000455          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFZC010000455 DAZFZC010000000
VERSION     DAZFZC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.471"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..297
                     /locus_tag="ACHKXP_005608"
     CDS             <1..297
                     /locus_tag="ACHKXP_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO7650350.1"
                     /translation="VSYVVPLAFVKNLEKTLTVNTGALLTMSVSVNGGTPPYKHAWKK
                     DGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSAEQPQSITSDACTVTVNGAGG"
BASE COUNT           90 a           68 c           96 g           68 t
ORIGIN      
        1 gtgtcctatg tggtaccgct ggcgtttgtg aaaaatctgg agaagacact taccgtgaat
       61 accggtgcgc tgctgacaat gtcagtcagt gtcaacgggg gaacgccgcc ttataaacac
      121 gcctggaaga aggatggtca gccggtagag ggacagacta ctgacacttt cagtaaagcc
      181 aatacgcagt caggtgataa gggggcttat acctgcgagg taacggattc tgcagaacag
      241 ccgcagagca ttacctctga tgcgtgtaca gtaacggtta atggtgcggg cggataaggc
      301 ttatggcaaa agatctgaaa ac
//
LOCUS       DAZFZC010000456          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFZC010000456 DAZFZC010000000
VERSION     DAZFZC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.472"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>320)
                     /locus_tag="ACHKXP_005609"
     CDS             complement(<1..>320)
                     /locus_tag="ACHKXP_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7650351.1"
                     /translation="DPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKM
                     RLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKL
                     SGGV"
BASE COUNT           61 a          102 c           77 g           80 t
ORIGIN      
        1 cactcctccg gacagcttcg ctgccagttc tttcagggtt ggccgggtcg tctcttcttc
       61 ccgggatttt gccagtacag ccagatcaag ctgccagcgt tgcacggaca cacgtaatgc
      121 cgcgtaggca tacaccaggc agtccagcgc ttcgttacgc cgctttttgt tatcccacag
      181 cagacgcatc tttccttttt cccacttctc cacaagctct tccgctacca gttgctgcgc
      241 ctctgtctgc gaaaaaatct ccggatcatc aggaaaacgg atggcatacg acgtggcttc
      301 atccgcagga gtgggatcgg
//
LOCUS       DAZFZC010000457          318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFZC010000457 DAZFZC010000000
VERSION     DAZFZC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.473"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..263
                     /locus_tag="ACHKXP_005610"
     CDS             <1..263
                     /locus_tag="ACHKXP_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7650352.1"
                     /translation="LKKLMQRLCGYGKHDDREHGELLTAQLRLGPADILESDENGIIP
                     EQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT           85 a           66 c           99 g           68 t
ORIGIN      
        1 tactaaaaaa acttatgcag cgtctgtgcg ggtacggaaa gcatgatgac cgtgaacacg
       61 gggagttact tacagcacaa ctgcgtctgg ggccggcaga catcctggag tcagatgaga
      121 atggtattat tccggagcag gccagggtaa tcacgcaggt ggtgatactg gatgcggata
      181 aaaagcagat acagtgtgtg gtaagaccgc tgcaaatcct gcgtgctgac gggacgtggg
      241 aaaatattgg cgggatgaag taacccgaca gcttcacaaa accggagtcc ggctccggtt
      301 tttgttgtca tgtccggt
//
LOCUS       DAZFZC010000458          316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFZC010000458 DAZFZC010000000
VERSION     DAZFZC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.474"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            221..316
                     /locus_tag="ACHKXP_005611"
                     /pseudo
     CDS             221..316
                     /locus_tag="ACHKXP_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           77 a           85 c           62 g           92 t
ORIGIN      
        1 taaacataaa atgcgaattg tttagtacaa aaagtgaact tctgatgatc tttccctgct
       61 accatggctc tttaccacag cagccggggt tgtttgggac cgacttccga cagcagatcc
      121 cggtgggcct gccaccatct cttcacaagc ttatgcttat tacatgcaaa atgtcccgtt
      181 ttgcatcttt gtgcctacgg cacacgatga tctttccctg aatctgcccc tgagaacctg
      241 aatcaaacgt ctgactcgca gaaccatctg tttctcgcgc tctgtagaac ttcatgaaaa
      301 agtcatcggt gctttt
//
LOCUS       DAZFZC010000459          313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFZC010000459 DAZFZC010000000
VERSION     DAZFZC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.denovo.475"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..>313
                     /gene="tssD"
                     /locus_tag="ACHKXP_005612"
     CDS             55..>313
                     /gene="tssD"
                     /locus_tag="ACHKXP_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO7650353.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDETFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMG"
BASE COUNT          108 a           49 c           62 g           94 t
ORIGIN      
        1 caagcataat agtgccattg ataaaatagc atgccataaa aggacttttc agggatgagt
       61 aatattgttt acctgacagt aacgggagaa caacaaggaa gcatctccgc aggttgtggg
      121 acttctgagt ctacaggtaa tcgttggcag agcgggcatg aggatgaaac atttacattc
      181 tcactcttaa ataatattaa taatacgggg cttggttcac agttccatgg tataacattt
      241 tgtaaattaa ttgataaaag cactccatta tttattaatt ccattaacaa taatgaacaa
      301 ttatttatgg gat
//
LOCUS       DAZFZC010000460        21365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.087, whole genome shotgun sequence.
ACCESSION   DAZFZC010000460 DAZFZC010000000
VERSION     DAZFZC010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.087"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..494
                     /locus_tag="ACHKXP_005613"
     CDS             <1..494
                     /locus_tag="ACHKXP_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7650354.1"
                     /translation="ITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQK
                     EMSPVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQ
                     ANGEADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFI
                     TVK"
     gene            586..1011
                     /locus_tag="ACHKXP_005614"
     CDS             586..1011
                     /locus_tag="ACHKXP_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650355.1"
                     /translation="MTAIKKLYDAANVALDVIDDEAAKGFPEPDWAHQLRNAIAEMNP
                     PDPTTDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            1067..1342
                     /locus_tag="ACHKXP_005615"
     CDS             1067..1342
                     /locus_tag="ACHKXP_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650356.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKADGLNDEETDALIQRILVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISDEDAAHLERILSISTFE"
     gene            1558..1893
                     /locus_tag="ACHKXP_005616"
     CDS             1558..1893
                     /locus_tag="ACHKXP_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650357.1"
                     /translation="MKAIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            1893..2105
                     /locus_tag="ACHKXP_005617"
     CDS             1893..2105
                     /locus_tag="ACHKXP_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650358.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(2685..3899)
                     /locus_tag="ACHKXP_005618"
     CDS             complement(2685..3899)
                     /locus_tag="ACHKXP_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO7650359.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNLDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKLDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            4101..4745
                     /locus_tag="ACHKXP_005619"
     CDS             4101..4745
                     /locus_tag="ACHKXP_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650360.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            5000..6085
                     /locus_tag="ACHKXP_005620"
     CDS             5000..6085
                     /locus_tag="ACHKXP_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFO7650361.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            6315..8231
                     /locus_tag="ACHKXP_005621"
     CDS             6315..8231
                     /locus_tag="ACHKXP_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7650362.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERFKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHENNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            8221..8964
                     /locus_tag="ACHKXP_005622"
     CDS             8221..8964
                     /locus_tag="ACHKXP_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650363.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NYPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            8974..9543
                     /locus_tag="ACHKXP_005623"
     CDS             8974..9543
                     /locus_tag="ACHKXP_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650364.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            9617..11932
                     /gene="nrdA"
                     /locus_tag="ACHKXP_005624"
     CDS             9617..11932
                     /gene="nrdA"
                     /locus_tag="ACHKXP_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7650365.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            12033..13175
                     /gene="nrdB"
                     /locus_tag="ACHKXP_005625"
     CDS             12033..13175
                     /gene="nrdB"
                     /locus_tag="ACHKXP_005625"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7650366.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            13253..14122
                     /locus_tag="ACHKXP_005626"
     CDS             13253..14122
                     /locus_tag="ACHKXP_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFO7650367.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            14294..15397
                     /locus_tag="ACHKXP_005627"
     CDS             14294..15397
                     /locus_tag="ACHKXP_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO7650368.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIDTFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTEAELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDEILILGYDPLPWIKVPVAV"
     gene            15399..15812
                     /locus_tag="ACHKXP_005628"
     CDS             15399..15812
                     /locus_tag="ACHKXP_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650369.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVSDADAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNSTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            15809..16285
                     /locus_tag="ACHKXP_005629"
     CDS             15809..16285
                     /locus_tag="ACHKXP_005629"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFO7650370.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            16285..16929
                     /locus_tag="ACHKXP_005630"
     CDS             16285..16929
                     /locus_tag="ACHKXP_005630"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7650371.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            16991..17410
                     /locus_tag="ACHKXP_005631"
     CDS             16991..17410
                     /locus_tag="ACHKXP_005631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650372.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            17420..17977
                     /locus_tag="ACHKXP_005632"
     CDS             17420..17977
                     /locus_tag="ACHKXP_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFO7650373.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMGACSAWIAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            18138..18947
                     /locus_tag="ACHKXP_005633"
     CDS             18138..18947
                     /locus_tag="ACHKXP_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650374.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVIKAESVESAENEPEQL
                     GFIEVEECVEVNDELKVQQSNDSNTNKIARTARGPRFTLSDKDDLFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            19130..19723
                     /locus_tag="ACHKXP_005634"
     CDS             19130..19723
                     /locus_tag="ACHKXP_005634"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7650375.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPIFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            19908..20138
                     /locus_tag="ACHKXP_005635"
     CDS             19908..20138
                     /locus_tag="ACHKXP_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650376.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            20668..21312
                     /gene="rnhA"
                     /locus_tag="ACHKXP_005636"
     CDS             20668..21312
                     /gene="rnhA"
                     /locus_tag="ACHKXP_005636"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO7650377.1"
                     /translation="MCARARKKIGAALDSGVYMTTKTPARSQAKTRKKAKNKNSPRTN
                     PTTPVVEFNPQLKTVKIFSDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRM
                     EMMGALIGLERLKYPCNVILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWK
                     RLDEAASRHNVRWKWVKGHAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNK"
BASE COUNT         5955 a         4615 c         5147 g         5648 t
ORIGIN      
        1 atatcaccga atcagatctg cgtcagaaaa ttgccgactc tctaaaccgt ttggccagcc
       61 gtatgaccac tgactcattt atcgacggtg gtaaggcgca attgctggac aacgcactga
      121 aagatattca gaaagagatg tctccggttg gtattgaggt actgagcctg tcatgggttg
      181 gaaagcctga ttacccaaaa actgtcattg aatctattaa cgccaaagta acggctaacc
      241 agcgtactct gcaacgtcag caggaagttg aacaacgtaa agctgaggcg aatatgctac
      301 gtgaacaggc taatggtgaa gctgatgcta tccgtgctcg tgcgcaagca gaagcagacg
      361 ccattcgtct gcgcggtgaa gctctgcgtc aaaacccgaa cgttatggaa ctggaagcca
      421 tcaataaatg gaatggccag ttaccgcagt acatgactca aggggctaac actcctttca
      481 ttacagtgaa ataactcccc taaaaagttc aggcgtccag ttggacgcct tttttatcgc
      541 aattatctta ttaagaaaac aatttgttta aaagaataag aaaacatgac agctattaag
      601 aaactctacg atgccgcaaa cgtggctctg gatgttattg atgatgaagc agcaaaaggc
      661 tttcctgaac ctgattgggc gcatcagcta cgaaacgcta tcgccgaaat gaatccacca
      721 gatccgacca ccgacgagac agactggcag cgatttatcc gtatgtacgc tcaggaaata
      781 ggtccaacgc caacggcaga gcaagcaatg ctgctgaaat acttcaaaga ggcgggagag
      841 gatttaccaa ttgatgactc agcatattgg ttccactgcg catggcgtaa gtatgacgtg
      901 atattcacac aaggcatggg gagcaaagat atggttgtgt ggcatctact ccatatagac
      961 acagccgttg acagagttat tgaacagttt ttccctaaac aagaagattg atcgcctatt
     1021 cataactaac aaaataagta aacactaacc acaaaaggaa aaacacatga gagttttagt
     1081 tcgaatcgtt accagcactg tctatgacgt gtttccgctt tttatggtca aagccgatgg
     1141 ccttaacgac gaagaaactg acgcgctgat ccagcgtatt ctcgttgaat atacaggtca
     1201 tgacgctgat tcagtgatgg ttgatgatga tggtgtttgt tggcataacg gcaactgttg
     1261 gtacgtagaa gagactcaac aaatcagtga tgaagatgcc gcacatcttg agcgtatttt
     1321 aagcatcagc acttttgagt gagtttacag taaaatttat ataagttagt gtctacctat
     1381 catgaagatt tttattgaat acttgttact catcgtttca atagcttttg tcatcgactg
     1441 cattttcacc ggtgtcattc gtaaagtctt ttccccggtg aacgacgtag tcataaacgc
     1501 tttggctatc gtgctcgtat ttaattcagc atttgatgta atcaaagagg tggcagcatg
     1561 aaggccatcc cattcgcgct gttgttcctt tcttcgatcg ttgtggccga caccactgtt
     1621 tatcagtgtg aaatgtctgt agccgacgtt aagaatggcg ctcttaccga cgtcataaaa
     1681 gcaccatatg gagcgatggt cgtagacagc ggcgaccagt tctatgttgt gcgtgacgat
     1741 cgagtgttgt catccccata tctcacaaat cgtaatggca aattaaccgg cgtcggagaa
     1801 gaccacttcg tatacaacaa atacaagggc ttctatggtg ttcacgcttc tcagcaaagc
     1861 taccttttcg atgactgcaa ggaggttgga taatggcatt aacactggca ggtctggaaa
     1921 tcgagaaaac aagcggctac tggcgtgcta agggtttcaa gcagcctggc attcttgagc
     1981 gtctggaacg tgaagatggg tatatcgtcc accagcggcg tgaatggcgt atgtacgatc
     2041 cagaaacagg aaaactgact acaaaagccg gaacactttg gggtctgtta aagaaaatac
     2101 actaaatgca aactgactgc ggtacgttcc gcagtcatat ttcatagtcg tcaccgctga
     2161 cagcatacac aatcaactac cgctgatagc atatcgagag tctatctcac cgctcacagc
     2221 atactttact cgatttttta ccgctgacag catacttaag acattgcatg aataatgtgt
     2281 accggtatgg gtataaccag aacaaaatta ccgctggcag catacgaagg tctgacatat
     2341 accattaatt accgctgata gcatatccaa acaaaaattc ctcaataaaa caccgctgac
     2401 agcatacgtt ctatcaggga gcagcaggca ataaatgcct ttcactacaa gcaatcagcg
     2461 caatagcaat agaatgttag tgagcgcaaa cctatatgga atgcactctt cgaggttagt
     2521 aaccactggg gaggtatgac agagcattga gtggtgatag atgatttacc gcccacagca
     2581 tacgttcatc tcactatacc gctggtagca tatatttaac cgtttacagc atacttttca
     2641 gaaaaatagc cgctgatagc atacatttca ccgctgacag catatcaaag cagtttgaga
     2701 ctattggaaa ggatctcaat catcttgata ttttcaggcg tcaaattctg cgaaagttcg
     2761 gttatcttgt tgataatgtt ctgtttggca tcaatttccc cagctttctc atctagtttt
     2821 ttgggttcga tgtcttcagg ttttggcggt gcgactttga gttttggatt gcggctgtga
     2881 atctggatat agatcgaccg ccctcgctta atctcgctgt attcgagata acccaaatct
     2941 tggagagctt ttaagccgtt gcgtatagtc tgattctgcg agctgacatt cctgctactc
     3001 aaattgagtc gcgcacgcaa tcgagcaagc gataccggcg caggcttggt tggaagactt
     3061 tcgatgaagg tgtacagagc ctgtgctgtt tctttgcgtg gtagcttatt gataaccttt
     3121 aactgcaaaa gaaccttatg gtcaaagcga tatagttcgg ccagcttcgg ttctgcatag
     3181 aacaccaccg tatctttctg ctcgttgtag tccacgctat tgatgaggtg caccatcaga
     3241 agcgagatct tgttagagcc gtcgacgttc ttttcttcat acgttctctg gaaagacaga
     3301 gttgtacgca tgatcttcaa aagactgttt gtaagccggt cgcggagtgt tttgcggatc
     3361 tgtgacgatg gatagccaca aaacttcgca aatttcgtga tgcttaactc gacacgaccg
     3421 ttaggttcgc cgtattctgc cagcgaacgc acaacgccca cccacgtttt gaaatcatga
     3481 tccatgtcga gacgaggacc ggttatcttg atatcggaat agccttcaga acgggctact
     3541 tcgagctgaa caagctcctt tgaagcatcg atctcatttg gcttgttacg cttgctgtat
     3601 tttgtcccct tgagcgtggg cacgaacaat cccagccgca tcaacgcaat tggttggact
     3661 gtattgttgc tattagggac aagttcccct gtgtacaatt caagggaacc ttcttcaaag
     3721 ttgtcgagat tatcttctac ttctttgtta tttttacctt ttttattttt tgtggacatg
     3781 tggacacctt tgtcattcaa ccgctgacag catacttgat ttgccgctga cagaatacca
     3841 aaaacagttg gcagcatacg gtgaaccgct gacagactat caattaccgc tgacagcata
     3901 catgaacatg gcttcagacc agtcgtggcg cggcttacag cgatcgggga tcttatttga
     3961 tctatacaag gatctatcta tggatctctt tattaggatc tatcctgtgg atatgtgaat
     4021 aattaaaaca ggcatttact accttcggcg cacctggtgg gttatcgttg cctcggctaa
     4081 caatcacaga aaaatgacat atggatctaa aacgcacgcg ctgggttcgt cgtcttgaag
     4141 acggctccta cactatcgaa tcaaattcca acctgaataa gcagaagttg ctttgtgaca
     4201 tctgcggtat agcggcgaag tgcccgatct acgaaaccag aattaaactt gataaggctg
     4261 gtgtgaattt tcatttaaac agttgcatca ggtacgttcc attactcgca tttcgtaaac
     4321 cgatcatcgg attggatgct ccctacttca acacactccg ttcaggtgtg acgtggcgag
     4381 atcgtttatc accagacaag ctgatttgcc tcgtatccgc agacacaggg aaaatcatcc
     4441 gttttgggaa agtagacaag gtttactcag gcccagtaga cgaaatgttg cggaagcaca
     4501 gccggtttaa tcatctctgt atgggtggtg agaaaatcga gaaagtagaa gaagtgatcc
     4561 gcaaatccta cggacacttt ctgaccaaag atagcctgct caccgcaatc tacatcagac
     4621 atgtaaaacg tgagttcgac ctcgaatacc acagcgaaga agagcttaac cttgttgacc
     4681 cacgtccaaa agctggcgtc ataagcataa acgcagcgcg taaaaagccc actgacgcgc
     4741 tgtaaccctc cagatcgtat attggcgtag atagaatcta cgccccctca aaatagctct
     4801 catagcgttc tacagtgatc ctgtcttatt tttagtcata cagacaagca aagttgcgcc
     4861 acgataaata ggtatatact tacttataaa ttttgtatat taaggcgctc gtttcattcc
     4921 taacataccg ttatgcatag ttgtttacct cctcattgct cttagaattt gtatcaaaat
     4981 aaccacaaag gaaaaataca tgactttgcc atacggcgtc atttctgact gccactacca
     5041 caaatgggat gcgttctcca cgacgaacgc tgaggggctt aactccagac ttgaaataca
     5101 gttggaagca acgaaagaag cagccatcgc catgaagaag gccggttgta agtacatgtt
     5161 ggttgccggt gatacatttc acgtccgagg aactgtgtcc ccttctgttt tgcattacgt
     5221 aactgaaacg tacaagtgga ttatcaacga gcttgatctg acagtagtaa tgctggccgg
     5281 taatcacgat cttgaaacca acgattcagt atatagcgcc aacgcagcag catcgctgag
     5341 ttctatcggc gtggtaatcg tatgtggcaa gcgcccacac tcaataaaaa ttggtgatgt
     5401 gactgtccac ctgattagct ggcgtaacaa tcatgcggag cttatcagcg atctgaaagc
     5461 attacgtaag agcgtagaag gtgataatca tgacgttgtt atccatacat ccattaacaa
     5521 agccattcca acaatgcctg acgtcggtat cgatgcgcag gagttaaagg atatcggctt
     5581 tcgtctcgtg cttagtgggc attaccacaa ccacaaagag gtcattcctg gagttatcag
     5641 tgtcggtgca ctgactcatc aaaattgggg agatgttgga tctctggctg gctacatgat
     5701 cgtaaacccg gacggcagtt tcagtcacta cgaaaccagt gcgcctaaat tcattaacct
     5761 ggaagatgat gttgctgatg accaaattcg cggcaactac gtgcgtttcc gcgccgtaat
     5821 tgagaacgat gaagaaggca ttaagtacca gaacatcctc aaaacaatgg gtgcaaaagg
     5881 tgtcgtgtgc aacttcatcc gtaagtcatc aatgatggaa gggacagcca gcacaactga
     5941 aaccagcaaa atcgatagcc tgggagagtc ggtatctgct tattgcaaga ttgtccacga
     6001 tactgacggc ggatttgatc tgagcaaatt ggatattttg tgtcaggaaa tcctcaccga
     6061 agcggagagt tcggaggctg tgtgaagcaa agtcgttatg ggagctttcg agactttgcc
     6121 atcacgatga aaagacttga acgaggccag acggtgatgt ttcacaagcc ctacccgcca
     6181 caaggaaatc ccgtagcgtt ttatcttgga aggttaacaa gaaaaggcgt attgaggcgc
     6241 agatccttcc cggcgcatac ggagttcaga ttgaaagaag gccaaaagct aacacacggt
     6301 atcagaggtg ttatatgaag tttttaaagc tccaggttga gaattttatg gctatcgcca
     6361 gcgcggaggt cgagttagat cagcgtggtt tagtgctcat tcagggtgtt aatagtgatg
     6421 atagttccgc atcaagtaat ggctctggaa agtcaactct aatgaatagc ctgatgtggt
     6481 gtctttatgg cgaaacagct catggtgtga agggtgacga tgtgttgtct accgaccatg
     6541 aaaagaactg tcgtgttgca gtaaccatcg aggatgaagg caagagatac gcaatcattc
     6601 gtcaccgtaa acacaaagag ttcaaaaatc gtcttatcgt tcgtggtgaa gatggcgata
     6661 tgacgaaagg caaagatgcg ctgacgcagg agttcgtcga gcgtctgatc ggtgcatcta
     6721 aagaggtttt catggcttcc atctatgcga gccaagaagc tatgccagat ttacctggaa
     6781 tgtccgacaa aaacctcaaa accatcgtag aagaagccgc tggcgttgac agactgacac
     6841 gcgcctacgc tattgctcgt gagcgagcta atgcagctgc cgcacgtatg gatgtggtta
     6901 aaaccaaatt ggagtcgaca atctcgacca ttgaggcaac acagtcagaa attgagtcag
     6961 cgaaagcctc ctctgaatca tgggagcaag agcgttttaa acgttatgac gatgccctgg
     7021 ctgggctggc cagtgccgaa gttgagttaa cggaagttga acttgagatc cgcactctgc
     7081 ccgaacagat acgtgatacc gagaaggcaa tcgaaagtga gcgcaaaaag ttagcctcaa
     7141 aagaagaaca tgacgccaag ttgctcaaag ttcgtggtgc gataactgat attcgggcaa
     7201 gcatcaaagc tacagaaaat agtcaggctg atgcaatgaa ccgcgcgcgt aattttaaga
     7261 ccaaagcaga agaggttggt actaaagtgg gatcaccatg ccctacttgt ggcaaagcct
     7321 actgcgaaga agatctatca acggtgaagg agaatttcat tgaacaagca cgacaggaaa
     7381 ttggtcaggc gaagacactt gcagaggcaa tggctaaaca caaaacgaat cttgagaaag
     7441 cgttaagcat tgagtctgcc cttgttaaaa cgacacctga tgtaacggct atcattgccc
     7501 ggattgaaga gcttacgaaa caactctcat ctttgcgtca tcgtgagaag gaggttgttg
     7561 ctattgagtc tcttgtgact cgtgctcgta ctgaggtcga tcgtatatca aaagagatta
     7621 atccgtttat tgctcttatc gccagacacg aaaataactt ggtatccagt aagtctacct
     7681 tcaagtcctt aaaagatgag ttgaaggcta ttcaggaaca aacgttgcta ttggaaaaag
     7741 ctcgtcaggt ctactctcct gccggggtgc gttctcatat tttgacgtct gttacgcctt
     7801 tcctgaatac acgcactgcc gagtatctca atacgttgtc tgacgggaat attactgctg
     7861 agtggtcgac gatggatgtc actaaaaaag gtgagtatcg cgacaaattc aacattagtg
     7921 tgcagaagaa aggttcaagt aagtcgttcc aaaccctctc tggtggtgag aagcggaagg
     7981 ttcgcattgc gtgttctttg gcattgcagg atctggttag taaccgggcg agtaaaaaca
     8041 tcgatttgtt tatcggcgac gaaattgacg atgcactcga tacagccggt cttgaacgcc
     8101 tcatgggtat tctggagtcc aaagctcgcg agcgaggtac tgtgctgatt atctcccata
     8161 aagagatgaa gtcatggttc cgggaaacta ttacgctgga agttaaagag gggcgcagct
     8221 atgtcgttta aattaagccg ctcgcagttt ttgcaggtat ttgcagtgat gcagtcgata
     8281 aaactgatca atgggcatac ttccaatggt gcggctccac gtattctgtg gggcagcaac
     8341 aatattgacg gagtacaatt cgccgcgttg cttggtctaa tatccgagac accattgatg
     8401 caaagtttga aatcactacc acctggatgt attgcgccga tcctgattaa tccttttgtt
     8461 gaggggggat atcttcccaa cgtcgggcct gggtttattg catcccatga aactgaagat
     8521 cttaacatta atagcgaagg gttctttggg ggaatgggtg cgcatcactg tatggctttc
     8581 acgaacctta ttcgacttgc caataagcgg gtggatagtt tggcatcgcc aggtgatgct
     8641 tttactggtt tccttatcca aaggagggat aaaaagtaca gtgcggacaa actacagttt
     8701 gttggtaagt atggagaaat ggtagaaatc gaacttcagc tccctcatgt tttagcaaac
     8761 gatagtgcag acagtcggag gctgttgggc atcatgcgtc atttcatagc aagtggcgtt
     8821 aaacatgccg tagataaacg tgtcacgcag gaaaatgagt attcagactt tgcaaactat
     8881 ccccaaccaa cgttgcaaac ggcaatagta accaattcgt tggaggcgag attattggaa
     8941 aaccctatat ggggaacatg gtaaggagac tatatgagta aaaaaatcag cgtagttggt
     9001 gttgatccct ctatgagcaa ctttgggctt gctgtgggca ctttagacct tgaaacggac
     9061 gaacttgaga ttcacggcct tactcttgtt gagactaaag cggggagtaa caaaaagacc
     9121 gttcgtgtga acagtgacga tctgcgccgt gccagtgaaa tatggcgtgt tgcgaagcca
     9181 atcattgata aggcgaatat ggttttttgt gaactaccgg ttgggagcca aaactctcgt
     9241 tcgcagacgt cttacggtat ttgtatcggt gtacttgcgt gtgtggataa gccattgatc
     9301 caggtcactc caaacgaaat caagcatttt gttggcaata aacttactac atcgaaagaa
     9361 gagattatcc agtgggctac gaaaaaacac cctaaagcac cgtggctgcg tcgtaagcaa
     9421 tctggacagg atgttctcgt gaacaaaaac gaacatttgg ctgatgcggt ggctgccatc
     9481 cataccggta tgcaaacgga tcagttccgc caggttcgcg atgttcttaa gtctctcatt
     9541 tgatttcatt gataggtaag tgcttatcta ttaacatgag ccactatatt tagtggccct
     9601 ctttatttgg tgatacatga taagcatcgt aaaacgtaac ggccaaacag agccgttatc
     9661 cgaagaaaaa tacaaccgcg tcgtaatgta tggcgtagaa ggcattcgtg gcgtaagcgc
     9721 atccgctgta gcaatgggag ctgcggccag catttttgat gggatgacca ccagccagtt
     9781 gcatgaggct ttggttaaat ctgccgctga tttgatctca ccagaagcac caaattactc
     9841 acaggtggct gcccgcctga acatttttaa aatccgcaaa gatgccttcg gtcgttacga
     9901 ctatccgaac ttctaccaac acattgtcaa gaacgttaac aagggcgttt atgacaagga
     9961 tttgctgaca cattattcgt ttgaagagat cgaagaactc ggcaattaca ttaagccgaa
    10021 acgtgacgat ctttttggct atgcagctac ggtgcagttg caaagcaaat acctcgttca
    10081 aaaccgtgtt actggtgaga ttcatgaggg gccgcaacat atctatatgc tggtaggcat
    10141 gtgtctgttc cagaattggg aagacgactg cgctggcaaa acacgtatgg agatggtcaa
    10201 aggtttctat gacgttacaa gtacgttcaa actgtctctg cccacaccaa tcatggccgg
    10261 cgtccgtact ccaacccgtc agttctccag ttgtgtgctg attgagtctg gcgatagtct
    10321 gaaagggatt agtgcagctt cagccgcaat tatcgactac gtttcacgtc gtgctggaat
    10381 tggtattggt tttggccgta tccgtgcgct gggcagcgag atccgcaatg gtgaagccac
    10441 ccataccgga gttattccat tcctgaagca tttccagacg gctgttaaat cttgttcgca
    10501 aggtggtgtt cgtggtggcg cagcaacagc gttttacccg atctggcatc ttgaagttga
    10561 aagtctgctg gtggtgaaaa ataaccgtgg tattgatgaa aaccgcgttc gccatcttga
    10621 ttacggcgtc atgagtaacc gtctaatgta ccgtcgactc gtcagaagcg agaacatcac
    10681 tctgttcagc ccgcatgatg tgcctgatat gtacgaagcc ttcttcacag accaggagct
    10741 gtttgaaaag ctgtaccata aatacgaagc cgatgattca attcgcaaga agtcagtacc
    10801 tgccattgag ctgttctcat ctctgatgca ggaacgagcg tccacgggcc gaatttatat
    10861 tgcgaacgta gatcatatta acgagcatgg cgctttcatt cctgctcttg cacctgttcg
    10921 ccagtcaaac ctgtgcatgg agatcactct acccactcgt ccactggcat ttaccgacga
    10981 cccgaacggt gagatcgcgc tatgcacttt atccgctttt aacctcggag ccatccgttc
    11041 actggagtct cttaaagagg tggcgttcta tgccgttgct gcactggatt cgttactgga
    11101 ttatcaagac tatccgatgg aggcagccga agtgcctgcc aaagctcgtc gtagcttggg
    11161 aattggtgta accaactttg cttattacct ggcaaagaat ggcgttcgtt attctgatac
    11221 cgctggcaat aaactggtgc atgaaacgtt cgaagctatc cagtattacc ttcttgatgc
    11281 cagctgccga cttgctgaag caaaaggtga gtgtgactgg tttgagcaga ccaagtacgc
    11341 aattggtcag ttgccgatcg accattaccg ttcttcatta gacgaaagtg gcgaaaccaa
    11401 ctttgagtta aagatgccgt gggaagaact gcgtgaacgt attgcaaaat acggccttcg
    11461 caactccaca ctgacggcac aaatgccatg cgagacttcc agccagatca ctaattccac
    11521 caacggcatc gaaccgcctc gtggcccggt gtcggtgaaa tcttctaagg acggcatcgt
    11581 taagatggtc gtgcctgagt ttgaaaaact gaaggaacag tatgaatacc tgtgggatat
    11641 gccggacaac cgcggctatc tgacaaaggt ggcgatcatc cagaagttct ttgaccaggc
    11701 tatttcagcc aataccaact atgacccttc tcgctttgaa ggcgataaag tcccaatgat
    11761 gacgctactg tcagatttgc ttctcgccta caagatggga gttaaaacgc tttactacca
    11821 caacaccaga gatggggcag gaaagcgtga tgacgacgaa ccgcagaatc cactgacgca
    11881 agctgtagcc gtcgagccag aagatgagtg cgacggagcc tgcaaaatct gacatatggt
    11941 ggggcatatc cccaccttat ctttgatttg taagccttgt ttaaacacat aagataacaa
    12001 cttgtttaaa cacatcaaaa aaggaaaaac acatgtcata ttcaacgttc cgtttgggtg
    12061 ctaatgatgc aaccaaagag cctatgttcc tcggacaatc tgtcaacgtg gcacgttacg
    12121 atcagcaaaa ataccgtgat tttgaaaagt tgattgaacg tcaattgtct ttcttctggc
    12181 ggccggaaga agttgatatt tcgagcgatc gtatcgactt caacacgaag ctgcgtgacc
    12241 acgaacgtca catttttctg agcaatctcc gttatcaaac gttactcgac tcagttcagg
    12301 gacgtagccc aaatgcaacg ctgctgccgc ttatctctat tcctgaactg gaaacgtggg
    12361 ttgaaacatg gtctttctct gagactatcc atagccgcag ctacacccac attattcgtg
    12421 gcatggtgga cgatccgagc attgtttttg acggtattgt tacggatgaa gaaatcatca
    12481 accgagcgat cagtatctct gctgaatatg acaggcttta tgggatgacc tgcgagcgcc
    12541 agtcgttagg tgagaaagag tttgaacgtc tgtacgtaaa tgaatatggc tgggagccat
    12601 accctttgca ccgtcagctt ttccgcacgt tggtgtccat taatgcgctt gaggcgatcc
    12661 gtttctatgt aagttttgca tgtacgtttg cctttggcga acggaagttg cttgagggta
    12721 acaccaaaat tatgcgcttt attgcccgtg atgaagctct gcattgcgaa ggaactgaac
    12781 gcatgatccg cttcatgcgt accggtcgcg aaggtttatt gtggaaagag attgctgctg
    12841 atgaagaaaa cgtcatttac gacaccatga aatcagtcgc cgaacaagaa atgaactggg
    12901 cagactatct cttcaaagac ggttcgatga ttggtttaaa cgcggatatt ctgaagacct
    12961 atgtaaaata ccgaaccaat ctggctatga atcgtcttgg cctgaaggct ttatttccag
    13021 aagttaccac agatccgctg gtctggatga acaagtggtt gttaaccgac acactgcaaa
    13081 ttgcaccaca agaggcagag caaagcacat atctggtagg tcagatcgat tctaccgtgg
    13141 acaaggcttc tctaagccag tttgcagacc tgtaaaccga tacaaagcat tatgtggcct
    13201 ggcaacgctg ggccacaatg gatcacaaga attaagaagg aacaaaacta gcatgaactt
    13261 taccaaactg actgaccacc tgaaacttgc caccgatcgt ctcattggat ttaagccaga
    13321 accatatgag ttgcatgaag gtcatggtgt agctactgaa agtatttaca agatggtcga
    13381 tcagtttcat gaactcttcc agcatccgag acgcgttatg ccgacaccag agctgcttcg
    13441 tctccgtgca agcctgattc atgaagaagc tgtagtggaa ggtattccag ctgcaatgaa
    13501 tggggatatt gagcaactgc tggatgcaat ggccgacttt ttatacgttg gtgttggtac
    13561 gatggtcgcc atcaaaggtg gtatttctac cggcatgacc tattacacgc aggaacagag
    13621 cattgatcgc tttatgcaga caatttttgt gcccggtaac actgttttcg atgatatggc
    13681 aatgccattt caggaagctc gtgaggcgtc atgtatgctc gaagagctgg cagataaact
    13741 tgagaacaag actgttaagg attctgagct gattcaggaa ctgcgccgtg tcatgaacaa
    13801 aatctatgtg gcgtgcatga tgacctatcg actggctgat ttcctcggta tcaatgtagt
    13861 cgagctggtt ggcgaaattc atcggtctaa catgacaaaa ttatggcctg ctgatgtaga
    13921 ggaacgtcgc caggctgtgg ccaactgcaa atacgactct tcagacctgg gatttcgcca
    13981 tgctgatggt accgataaga tgatcggttt tcgaatttcc gatggaaaga ttctgaagtc
    14041 tccaacctat agtgatgtcg atttatcctc ctttgttgag caagctaaag cctcagcaat
    14101 gtacggaatg atcaaaaaat aattgtaggt agttatctat ctatgtatat tgtgttggcg
    14161 cgttaaattc ctgaaacaac tattcgtttt tggtggccta tggccaccat ttttttatct
    14221 atctggcctt gttccctcaa taaatgtaaa ctcacgtaat gaataagtgg ttacttatct
    14281 ttgtgaggtt tttgtgtcac tccttttgaa tcgtgagcat acgaacggtc aggtaacaaa
    14341 cgcatcgtat gcaaaagtta ttgagacggt gcttaaaagc ggcgtgcagg ctgatgatcg
    14401 cacaggcact ggtactttaa gcacctgcta cgttccctct tactacatgc ttactggtgg
    14461 gactgtgccg cttatttctg gaaaggcggt aaatcttaag ccactgcttg tcgaacttga
    14521 gtggtatctg aaaggcacgg gcaacatcca atttctcaag gataacggcg ttaagatttg
    14581 ggatgcatgg gccgatgaga atggcgattt ggggccggtt tacggtaagc agtggcgtcg
    14641 atgggaagat acccgcatcg tgagccatag tgaatatctg agcaagatcg atactttccg
    14701 tgaacgcggg tacaaagtcg agggatacct gggtatcagt gaagatcgcg tagtgctgtc
    14761 ccgtgaaatc gatcagctac agcgtattgt cgatacactg cgcacgaacc ctaccgatcg
    14821 tcgcatcatg cttaacgcat ggaacgtagg cgagcttgag gatatgaaac tgccaccttg
    14881 ccactttgtc ttctctttgt ggagtcgtga gctggatttt gaaacccgtt taacgatggc
    14941 aactgacatt ggtcttcaac acagtcgcct cggttacgag tctatctaca ccaagatgct
    15001 atacgatctg gagatggacg gcagtgttac tgaagctgaa ctggatgaac ttggaatccc
    15061 caaacgcatc ctcaactcct gcctcgtaca gcgtagcgta gacacttttg ttggtatgcc
    15121 attcaatatt gctggctatg gcattctcac tcattttctc gcgaagatta cgggtcacat
    15181 ggccggtgca tttgtgcatt ttggctttga cgtgcatttg tacaacaacc acatggaagg
    15241 tgtgtgtgag ctaatgaaac gacaagctcc agagcattca gatccggtcg ttattttccc
    15301 tcatgaatgg tcagagttgg atgatttcaa atgggacgag attttaattc ttggctatga
    15361 ccctctaccg tggatcaagg ttccagtggc ggtgtgatat ggcaagaggt atgtatgtct
    15421 tatgcgaaat tgaaggtgtg ctggcaaata ccagtcatcg taaatctgta tctgacgcgg
    15481 atgcaggcca gctcattgcc ggtgatgaac tcattttccc caccagccgt atgttgcgtg
    15541 gttttgctcg ctcaggggct gaagtggtgc ttatcagtag ccgctctgaa actcttgaag
    15601 cgcccactaa acgatggctg aaagattttg gcgttgatta cgactggctt catctcgtac
    15661 cgaatagcac cagttatgag aagcatatta aacgcacatt agcggagcat aaaggcgatc
    15721 tgcttatcgc tgcgctggtg cacgatcctc gactccgtgc cgctttagct gactctcatc
    15781 atagaccggt catctatgag gtgagcaaat gaagatgata gctgctgttg gccgtaacta
    15841 tgagatcggc atagcgaacg aactgccctg gcgttgttct accgatctga agctatttaa
    15901 gagactcacc aaaaacgcca ctgtcgttat ggggcgtaaa acgatggaaa gtctcaaacg
    15961 ccctcttcca gagcgtcata acctcgtttt gacgcgctct catggctttg taccaaatgg
    16021 attctaccct gctggtgtgg atgatgtgtt gcgattacca gagcctgtgt gggtgattgg
    16081 cggggaacaa atttactcgc tattcatgcc gcatgttgaa gagatttggc tctcccacat
    16141 cggcgttgat gtgccaaacg ccgatgcatt cttcccggca agcatgatgc gtaatttagg
    16201 ctttgtgcct gttgaaacag tttttaccca acgagccagc gaggaagagc ctggcttttc
    16261 gcagatcgta tacagaaggt cgtaatggat taccggattg ggatcactgg tgctcagggc
    16321 agtgggaaaa caaccctggc aaaatatatc gacaaacatt acggaatccc ttacgtggat
    16381 gctggggtcg gaagtttgat gagccgactc ggtgttcgag taggtgagtc tatgcctcta
    16441 tatgagcggc ttcagattca aatggaaata gcaaagcata tagagctact tacgcgtggt
    16501 gttgaaggct ttgttatcga tcgcacacct gctgatgtta tggcctacac gttggatttg
    16561 gtcggccata ccaatgaaga tcgatgtatt gagttagccc tcgatatcga aaagttttgc
    16621 cacaaaactg ctatttcaaa ctttaacgcc attgctggcc tacgcccggg ggtcgctctc
    16681 tcagagcgag attacttgcg gtcacaacga gcatcattag accgtctgta tgtcgctcgt
    16741 attgatgcgt tgatgtgcgg ggaactgaca aaaattcacc tgcatccgca aaggggagat
    16801 ctgcaaacct tcgtcgtttc caaccggtat cgcacagttg aagcaagagc cagatcagta
    16861 atgagaatgc tcgataacgc ggtagaaaag atagaaaacc gcttctgtgg ccgagtaacc
    16921 gttcattaga aattgttcgc ctcttcgaca ttgcgacaat aaaactctca aaatgggtta
    16981 aggataaaaa atgtttagtg aaatgttgct tgaagatgaa ctggatcgga aaacaacaga
    17041 ggctttgatt cgtgtagcgg acgaacattc ccggtcgctt atgagcgatc gagaggctcg
    17101 tctggctatt cgtgccatat tcgaaactgc gcaggggctt gttggcgcac aagtaggtga
    17161 agccattaac atcgccatgt ctcagttcag tgaagacagt aaaaagcctc tgtttcctat
    17221 gcatttgatg ctggctggtg gcacggtgct ttatatctct gtttgtctgg atagcaacca
    17281 aatcaatatt ctcaacactg cgtcaggtaa gtggaaagat ccgattgtct gtgaaaccag
    17341 tgaagaaact ttgaaaaaag cggctcaatt tgtacgtagc gcactactta agggcgctaa
    17401 gaagttgtaa ggagttctga tgacaacgat tgttgcaggc atcgatatcg agtctacggg
    17461 actggatttc cttgctggtc ataaaattat tgaaatcgca attacccgct atgaactgga
    17521 gacacagaga catattgata gtctggagat gcgttttaac cctcgcagaa acatagatcc
    17581 gaaagctcaa gccgttcatg gcatttcatt agaacagctc gcagctgaac ctttgttgtc
    17641 aaatcatgcc agcgaaattg gcgcttatat gggggcatgt agtgcgtgga ttgctcataa
    17701 cggcgaagcg tttgatatac catttattcg acacgagttt tcagggtatg gagtaagact
    17761 gccagacgtt cccgttatag atactatgtt atcgggattg tgggccacag aagacggtaa
    17821 acgtcctcgc cttgaagaat tggccttctc tcttggcttt atatacgatc atgccaaagc
    17881 acatagtgcc ttatatgaca caaacttaat gatgcaatgc ttctttaagg cacgtaataa
    17941 atacggattt tttaaattac cctctgaaat tgtgtaaaac aaaagcctgc tttaaagaga
    18001 gtttgaagca ggctttcttt taaagaacag tcgcctttca atcatttcct gcctgtattt
    18061 aatacttttc cgcctgatag gtttagtcaa aatgtagcca tcgaaacgca aatgcaacta
    18121 aacagaagga gacttacatg agttcggttg aaaatgtaat gacaaatgat gatctggatg
    18181 agctgacagc catgttgcaa tcacttgatg aaccagtaaa aaaagctgca caggttgaaa
    18241 atactgatga tattgacgat ctgcttctcg gcctcgatgc tggcgtagcc atgagttctg
    18301 atgatgttgc cgaagaactg ttcaatgaag aaaaagcagg tgatttcagc tccgctttaa
    18361 atgagttgga gttagctcat gagcctataa acgtaattaa ggctgaaagt gttgaatctg
    18421 ccgaaaacga gccagaacaa ttgggattta ttgaggttga agagtgtgtt gaggttaatg
    18481 atgaattaaa agttcaacag tcaaatgata gcaatacaaa taaaatagct cgtactgcaa
    18541 gaggtcctcg ttttacttta agtgataaag atgatttgtt tttcaataaa gcaggcttag
    18601 aaaaagatat tttcttagac gcttacgaaa acgcgcctgt caaagcaaag gataagatat
    18661 taaaccttct taattggttt agcggaggtc cagatattag tgtttacacg gtaatttcca
    18721 tgagacacct tctcacagaa aagaaggcta caagtaatag tattaagatt gctttaatga
    18781 gcaatccaga aaaaccgtat ccgcttaaca ctgcgtcaac tcaggctggg caaatgatgg
    18841 ctgtatttcc agctacagga attgctgtta gagacggtgg aaatctaaca ttgaacgaag
    18901 aatcaccgat cgttaagaag tttgtcgcgg agtacactat tggatgacgt tcccctactg
    18961 aaaataaagc ccatagagag ctttatagtg ctgggtaagc taacccatac ccagcaccac
    19021 aaaaacgcgc cagagagctt cttgttcgca tttctggcgc gttttatttt attgccatac
    19081 ataaaatcaa atgcaaaaat aggtaatcac ttacttaccg agaaagaaga tgattgcagc
    19141 cgaaaaaatc aaacagcgaa agcgcgacaa ctctcttcgt gacctctgga gaacacctga
    19201 ttggctgttt tctgccattc aacgttatct tggagtgaca tttgatgttg acgttgcctg
    19261 caacaaggac aatgcaaagc tgcctatttt cataggcgtt gagcgcgatg ctttgaaatc
    19321 tgaatggggg cagccaggta caattgcctt cctcaatcca ccctactcca aaatctcccc
    19381 ctggattgat gcggctatac gtgagcaggc tcgcggagtt acaacagtga tgctaattcc
    19441 tcaatccctc gatacaaagt ggtatgagcg tgcaacagag tgtgcgaatg agacgattat
    19501 tctgtctggt ggccgcgtag cgtttgtcga gcctgacgtc aatttgggtc aggtagaagt
    19561 aaacatcaac cccggtggca gtatgctcgt tgtttttcga ggattctgtc aggacgctgg
    19621 gcactctata agcaagatcc ctttggacgt catgaaaagt ctgggagggt atgatcctgc
    19681 gaatgtgatc aggaaaaaaa gaccatcaaa gaaggctgct tagtttgttc tggcgtctgt
    19741 aattagcctg cttctgtata taaataacta catattaatt attaatatac ggaagcaggc
    19801 tgttttatat cagagactcc cagacctgaa caccactaca gaatccacta gaaccccttc
    19861 ccagacgctt taaaatcgat tttatgaacc actttaagga aaccaacatg tcatacccga
    19921 ctaatgtcgt tgcgctcgta gagagcgatt ttctggccca ggctcgtgaa atgatgaaag
    19981 atcgtgagca ggctttcaac ttgtacgagt gggcaattaa gtgcttgcat cttggcgagc
    20041 atcgcgaact tgttgaacag cttttaggtg agttgatcaa tgaggtgttt gccttgaatg
    20101 ttcaactaca tggtcgagaa aataatcaat caaaatgata gataagtaca aactattcat
    20161 aaagtgaatt gtaagtgcta agatctgata gtttccagtc gtagactgga ggctcgacct
    20221 gatgggtggg ggtaagcgtc actggcgtca ggtttaaaaa agctcactac cagcgtagaa
    20281 ccggtgccgt ataggtgtcg gggaaggggg aaccaaagtg agcggagaca agggtcactt
    20341 tatgattgtc gagtctgggg tgtttcgaga ggttgaatcc agtactcccc ttcataaagt
    20401 gtgggaagat ctcggttctg gggtgctgtc atccataact tcccaagtct aagctggcag
    20461 tagacttaga ccatagcttt tcaggttatg aaacgaccag gttggtgagg aattttatac
    20521 tcacctccct gggagagtat tacctgaaaa gacaacctct cacttcgttc gaggtgaact
    20581 tcactcactt cgttcgttca gttcaggttt ataaaaacct gttctgggaa gtaatttgtt
    20641 tattttaata attattaaca cgcacgcgtg tgcgcacgcg cgaggaaaaa aatcggcgcg
    20701 gcgcttgatt caggagttta tatgacgacg aagacaccag cccgatcgca agcaaaaact
    20761 cgcaaaaaag ccaaaaacaa aaattctccc cgcaccaatc ccacaacgcc tgtcgtagag
    20821 ttcaatcccc agcttaaaac cgtgaaaatc ttcagtgatg gctcttgcct taaaaatccg
    20881 ggtggcccgg gcggttacgg tatcgttctt cagtatcgtg gagaggaacg cgagttctca
    20941 gatggttttc atagcaccac caataaccgc atggagatga tgggggcact tatcgggctg
    21001 gagcgtttga aatatccatg caacgttatt ttgcactctg atagccagta tctgaaaaac
    21061 ggcatgacac agtggatgaa atggtggaaa cgcaatggat ggatgacttc tgacaaaaaa
    21121 ccggtaaaga atgttgatct gtggaagcgt ctggatgagg ccgcaagtcg acataatgtt
    21181 cgctggaagt gggttaaagg tcacgccggg catcgtgaaa atgaaatatg tgatcgactc
    21241 gcgaagatcg cagctttttc agcagcagat atgcctcaca agaaagatat tggttttgtt
    21301 tataacaagt agtaagtaag tgtttaccta tcattttaaa tcatgtatct tatcggcgtc
    21361 aggat
//
LOCUS       DAZFZC010000461         9241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.149, whole genome shotgun sequence.
ACCESSION   DAZFZC010000461 DAZFZC010000000
VERSION     DAZFZC010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.149"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            183..1991
                     /gene="frdA"
                     /locus_tag="ACHKXP_005637"
     CDS             183..1991
                     /gene="frdA"
                     /locus_tag="ACHKXP_005637"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7650378.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            1984..2718
                     /gene="frdB"
                     /locus_tag="ACHKXP_005638"
     CDS             1984..2718
                     /gene="frdB"
                     /locus_tag="ACHKXP_005638"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7650379.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2729..3124
                     /gene="frdC"
                     /locus_tag="ACHKXP_005639"
     CDS             2729..3124
                     /gene="frdC"
                     /locus_tag="ACHKXP_005639"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7650380.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3135..3494
                     /gene="frdD"
                     /locus_tag="ACHKXP_005640"
     CDS             3135..3494
                     /gene="frdD"
                     /locus_tag="ACHKXP_005640"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7650381.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3557..4690
                     /locus_tag="ACHKXP_005641"
     CDS             3557..4690
                     /locus_tag="ACHKXP_005641"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7650382.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQVAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4779..5315
                     /gene="blc"
                     /locus_tag="ACHKXP_005642"
     CDS             4779..5315
                     /gene="blc"
                     /locus_tag="ACHKXP_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7650383.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGSY"
     gene            complement(5309..5626)
                     /gene="sugE"
                     /locus_tag="ACHKXP_005643"
     CDS             complement(5309..5626)
                     /gene="sugE"
                     /locus_tag="ACHKXP_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7650384.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STQ"
     gene            complement(5802..5948)
                     /gene="ecnB"
                     /locus_tag="ACHKXP_005644"
     CDS             complement(5802..5948)
                     /gene="ecnB"
                     /locus_tag="ACHKXP_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7650385.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6059..6184)
                     /gene="ecnA"
                     /locus_tag="ACHKXP_005645"
     CDS             complement(6059..6184)
                     /gene="ecnA"
                     /locus_tag="ACHKXP_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7650386.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(6236..6802)
                     /gene="efp"
                     /locus_tag="ACHKXP_005646"
     CDS             complement(6236..6802)
                     /gene="efp"
                     /locus_tag="ACHKXP_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7650387.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6844..7872
                     /gene="epmB"
                     /locus_tag="ACHKXP_005647"
     CDS             6844..7872
                     /gene="epmB"
                     /locus_tag="ACHKXP_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7650388.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENKGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARVTDALVERFSHTTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            8267..9136
                     /locus_tag="ACHKXP_005648"
     CDS             8267..9136
                     /locus_tag="ACHKXP_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7650389.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         2364 a         2326 c         2369 g         2182 t
ORIGIN      
        1 tcagtgataa tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt
       61 atccatcaga ctatactgtt gtacctataa aggagcagtg gaatagcgtt cgcagaccgt
      121 aactttcagg cacttaccct gaagtacggg gctgtgggat aaaaacaatc tggaggaatg
      181 tcgtgcaaac ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg
      241 ctgcaattgc tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat
      301 acccgatgcg tagccatacc gttgctgcag aagggggctc cgccgctgtc gcgcaggatc
      361 atgacagctt cgaatatcac tttcacgata cagtagcggg tggcgactgg ttgtgtgagc
      421 aggatgtcgt ggattatttc gtccaccact gcccaaccga aatgacccaa ctggaactgt
      481 gggggtgccc atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca
      541 tgaaaatcga gcgtacctgg ttcgccgccg ataagaccgg cttccatatg ctgcacacgc
      601 tgttccagac ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg
      661 atattctggt tgatgatggt catgttcgcg gcctggtagc aatgaacatg atggaaggca
      721 cgctggtgca gatccgtgct aacgcggtcg ttatggctac cggcggtgcg ggtcgcgttt
      781 atcgttacaa caccaacggc ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc
      841 acggcgttcc gctgcgtgac atggaattcg ttcagtatca cccaaccggt ctgccaggtt
      901 ccggtatcct gatgaccgaa ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg
      961 gctaccgtta tctgcaagat tacggcatgg gcccggaaac tccgctgggc gagccgaaaa
     1021 acaaatatat ggaactgggt ccacgcgaca aagtttctca ggccttctgg cacgaatggc
     1081 gtaaaggcaa caccatctcc acgccgcgtg gtgatgtggt ttatctcgac ctgcgtcacc
     1141 tcggcgagaa aaaactgcat gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg
     1201 ttggcgtcga tccggttaaa gaaccgattc cggtacgtcc gaccgcacac tacaccatgg
     1261 gcggtatcga aaccgatcag aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg
     1321 aatgttcctc tgttggtctg cacggtgcaa accgtctggg ctccaactcc ctggcggaac
     1381 tggtggtctt cggccgtctg gccggtgaac aagcgacaga gcgtgcagca actgccggta
     1441 atggcaacga agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc
     1501 tggttaacca ggatggcggc gaaaactggg cgaagatccg cgacgaaatg ggcctggcaa
     1561 tggaagaagg ctgcggtatc taccgtacgc cggaactgat gcagaaaacc atcgacaagc
     1621 tggctgagct gcaggaacgc ttcaagcgcg tgcgcatcac cgacacttcc agcgtgttca
     1681 acaccgacct gctctacacc attgaactgg gccacggtct gaacgttgct gaatgtatgg
     1741 cgcactccgc aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt
     1801 gcaccgagcg tgacgacgtc aacttcctca aacacaccct cgccttccgc gatgctgatg
     1861 gcacgactcg cctggagtac agcgacgtga agattactac gctgccgcca gctaaacgcg
     1921 tttacggtgg cgaagcggat gcagccgata aggcggaagc agccaataag aaggagaagg
     1981 cgaatggctg agatgaaaaa cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat
     2041 accgcaccgc atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat
     2101 gcgctgggct acatcaaaga caacctggca ccggacctga gctaccgctg gtcctgccgt
     2161 atggcgattt gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa
     2221 accttcctgc gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt
     2281 gaacgcgatc tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg
     2341 tacatcatcg gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag
     2401 atggcgaagt atcaccagtt ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc
     2461 ccgcagtttg gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt
     2521 tataacgaag atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag
     2581 aacggcgtat ggagctgtac tttcgtgggc tattgctccg aagtctgccc gaaacacgtc
     2641 gatccggctg cggccattca gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg
     2701 accctgaaac cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc
     2761 aatgacgtcc acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg
     2821 cacggcggtt ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa
     2881 aaatggcccg gaagcctggg cgggattcgt cgacttttta caaaacccgg ttattgtgat
     2941 cattaacctg atcactctgg cggcagctct gctgcacacc aaaacctggt ttgaactggc
     3001 accgaaagcg gccaatatca ttgtaaaaga cgaaaaaatg ggtccagagc caattatcaa
     3061 aagtctctgg gcggtaactg tggttgccac catcgtaatc ctgtttgttg ccctgtactg
     3121 gtaaggagcc tgagatgatc aatccaaatc caaagcgttc tgacgaaccg gtattctggg
     3181 gcctcttcgg ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg
     3241 tgggtattct gctgccgctg gggctgtttc cgggtgatgc actgagctac gagcgcgttc
     3301 tggcgttcgc gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc
     3361 tgtggtgtgg tttacaccgt atgcaccacg cgatgcacga tctgaaaatc cacgtacctg
     3421 cgggcaaatg ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggtg
     3481 tcgttacaat ctaacgcatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt
     3541 atggaaacca gaccctatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg
     3601 ctccacattt gctgcccccc aaaaaatcaa cgatattgtg catcgcacaa ttaccccgct
     3661 tatagagcaa caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta
     3721 ttactttacc tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt
     3781 gtttgagtta ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg acgctattgc
     3841 tcgaggggaa atcaagttaa gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa
     3901 acagtggaat gggatcacac tattacatct cgcaacctac actgctggcg gcctgccatt
     3961 gcaggtgccg gatgaagtga aatcctcaag cgacttgctg cgcttctatc aaaactggca
     4021 gcctgcatgg gctccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg
     4081 cgcactggct gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt
     4141 ccagccactc aaactcaacc atacatggat taatgtaccg cccgcagaag aaaagaatta
     4201 cgcctgggga tatcgcgaag gtaaggcagt gcatgtttcg ccaggggcgt tagatgctga
     4261 agcttatggt gtgaagtcga ccattgaaga tatggcccgc tgggtgcaaa tcaatttaaa
     4321 accccttgat atcaatgaga aaacgcttca acaagggata caagtggcac aatctcgcta
     4381 ctggcaaacc ggcgatatgt atcagggtct gggctgggaa atgctggact ggccggtaaa
     4441 tcctgacatc atcgttaacg gcagtgacaa taaaattgca ctggcagcac gccccgtaaa
     4501 agcgattacg cccccaactc ctgcagtacg cgcatcatgg gtacataaaa caggggcgac
     4561 cggcggattt ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct
     4621 ggctaacaaa aactatccca atccagcgag agtcgccgcc gcctggcaga ttctcaacgc
     4681 tctgcagtaa aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg
     4741 tcatctacac ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg
     4801 ccgcagcgac agctgcattt ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg
     4861 tgaccgtagt gaataatttc gatgccaaac gttatctcgg tacctggtat gagattgccc
     4921 gttttgatca ccgctttgaa cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg
     4981 atgacggcgg cctgaatgtc attaataaag gctataaccc tgacagagga atgtggcaac
     5041 agagtgaagg gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtgtcat
     5101 tctttggtcc tttctatggc ggttataacg tcattgcact tgatcgggaa taccgccatg
     5161 cgctggtttg cggcccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt
     5221 ctgacgaagt gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta
     5281 agtttatttg ggtacagcag cccggtagtt attgagtgct gagtttcaga ccaataatcc
     5341 ccaatacgat taacgccaga ctcgccaggc gcatcgggtt agcggactca ccgagcagta
     5401 caatgccggt gatggccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc
     5461 ctactggtaa cgatttcatc gcccaggcaa gtagcgccat actgacaatc atcgccgtca
     5521 cagtaataac actcggcgtc aaacgactaa agccgtgggt atatttcagg ccaacggccc
     5581 ataccacttc cagcagacca gcaataacta agataatcca ggacatatca ggctccggag
     5641 aattggggcc gtccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt
     5701 gaacggatat tttgccaggt ggcaggggaa tttcaactat tacctttatg actatcacag
     5761 aaaaaacaaa cggcacgaca cagctgtcgt accgttattg cttattgctg cgctttcgtt
     5821 gctgcgccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     5881 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     5941 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     6001 atagacaaca tcaccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     6061 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     6121 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     6181 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     6241 taacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     6301 caaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     6361 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     6421 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     6481 gcaaccattt agcgttatcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     6541 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     6601 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     6661 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     6721 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     6781 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     6841 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     6901 gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa
     6961 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     7021 atcgatcgta tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg
     7081 caagatgagt ttgtcgtcgc gcccggattc tccaccgacc cgctggaaga acagcacagc
     7141 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     7201 tgcgcggtaa attgccgtta ttgcttccgt cgccatttcc cctatgccga aaataagggc
     7261 aacaagcgta actggcaaac tgcacttgag tatgttgctg cacatccgga actggacgag
     7321 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     7381 acgcaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     7441 gtgatcccgg cacgcgtcac tgatgcgctg gttgaacgct tttcacatac tacgctgcaa
     7501 atcttgctgg tgaatcacat caaccatgcc aatgaggtag atgaaacatt ccgtcaggca
     7561 atggctaagt tgcgccgggt aggcgttact ttgctgaacc agagtgttct gttacgtggc
     7621 gtgaacgata acgcgcaaac actggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     7681 atgccgtatt acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt
     7741 gatgacgaag cacggcagat tatgcgtgag ttgctgacac tggtgtcggg atatctggtg
     7801 ccgaaactgg cgcgtgaaat tggtggcgaa cccagcaaaa cgccgttgga tctccagcta
     7861 cgccagcagt aactgattaa acagggaagt taaacattaa aattgcctga tgcgctacgc
     7921 ttatcaggcc tacatcgtgc attgaaattg aatggatttg caatgaactg taggccggat
     7981 aaggcgttta cgccgcatcc ggcaagagca acaaacgcaa gtacagcaac ctgtcattaa
     8041 actgagaaat ttaatctttg cacgttgcat aacctttaat tattcatatg cattgctgtt
     8101 gttacttttt atatagctaa caccaataat taacctttct tttctgttta tatagagtga
     8161 gcacggctat ttatgttatt ttaacaacta ttactaccaa gcccatttat ctggcttata
     8221 aattaaaatt aattatttaa ttgagaaatg gttagggaga acctacatgg ccataagcat
     8281 caagggtgtt aataccggtg ttattcgcaa gagtaataat ttcattgcac tggcgctgaa
     8341 aattaaagaa cctcgcaata aagaatcgtt gttctttatg tctgtcatgg aactgcgcga
     8401 cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg ctgctgcacg
     8461 ccttcagtat gagcaagctc gcgataaagt cattaagaaa atggcagaaa acatcccaga
     8521 aattctggtt gatgagctta aaaatgccga tattaatcgt cgggttaaca cgctggaact
     8581 taccgataat caaggcgaaa atcttacctt cgtattaacg ctacatgacg gtagcaaatg
     8641 tgagttagtc gtcaatgagt tgcagattga aatgctggcg cgagcaatca ttcatgcaat
     8701 taataatgcg gaaatgcgtg aactggcatt acgtattacc tcactgttag atttcctgcc
     8761 gttatatgac gttgactgcc aggaaaatgg caatctggaa tatgacacct attcccaacc
     8821 ggaatggaaa cataacttgt tcgaccatta tctggcggtg ctttaccgct ttaaagatga
     8881 aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa
     8941 agaaatagag gcgatcactc gccgaatgct tgatttcagc ccacgactga aaaaactcgc
     9001 gggtgtgcca tgtcaggtct atgttcgcac cgtagcggcg aataacgccc aaccgctaac
     9061 ccaggatcaa tgcttacgcg cgctgcatca cttacgtgtt caatccacca gcaaaactgc
     9121 accacaggcg aaataatctc atctttgcta atgcgaggct ggagccatcc agcctgaaat
     9181 gactgataac gtgatcgttg gtcatgccgg atgcggcgta aacgccttat ccggcctaca
     9241 a
//
LOCUS       DAZFZC010000462         4757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.211, whole genome shotgun sequence.
ACCESSION   DAZFZC010000462 DAZFZC010000000
VERSION     DAZFZC010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.211"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..120
                     /locus_tag="ACHKXP_005649"
                     /pseudo
     CDS             40..120
                     /locus_tag="ACHKXP_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            119..640
                     /gene="prfH"
                     /locus_tag="ACHKXP_005650"
                     /pseudo
     CDS             119..640
                     /gene="prfH"
                     /locus_tag="ACHKXP_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(697..2154)
                     /gene="pepD"
                     /locus_tag="ACHKXP_005651"
     CDS             complement(697..2154)
                     /gene="pepD"
                     /locus_tag="ACHKXP_005651"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7650390.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            2415..2873
                     /gene="gpt"
                     /locus_tag="ACHKXP_005652"
     CDS             2415..2873
                     /gene="gpt"
                     /locus_tag="ACHKXP_005652"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7650391.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2965..4209
                     /gene="frsA"
                     /locus_tag="ACHKXP_005653"
     CDS             2965..4209
                     /gene="frsA"
                     /locus_tag="ACHKXP_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7650392.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4267..4668
                     /gene="crl"
                     /locus_tag="ACHKXP_005654"
     CDS             4267..4668
                     /gene="crl"
                     /locus_tag="ACHKXP_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7650393.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         1143 a         1179 c         1262 g         1173 t
ORIGIN      
        1 ctgaacataa aacactatca ataagttgga gtcattaccg tagtggggat gccagattta
       61 caccccggac gcggctaccc gattggcgca gcgttcttct ctgttggtcg tttttacccg
      121 acaagacgtc gctgtaacgg tgctggaaac agaaacgggc cgctactctg acacgctgcg
      181 ttcggcgctg gtttctctgg atggcgataa cgcatgggca ttaagcgaaa gctggtgtgg
      241 cactattcag tggatttgtc cgagtccgta tcggcctcat catgggcgca aaaactggtt
      301 tctgggcatt gggcgtttta ccgctgatga gaaggaacaa tcggatgcaa tccgttatga
      361 gacgctgcgt tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg
      421 cgccacgcat ctggcatccg gtattagcgt gaaggttcag tcagagcgca gccagcatgc
      481 taacaaacgg ctggcgcgat tgctgattgc ctggaagctg gagcagcagc aacaggaaaa
      541 tagcgcggcg ctgaaatcgc agcggcgaat gttccatcac cagattgaac gtggcaaccc
      601 gcgacggaca tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga
      661 aggcgattaa acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag
      721 cagttcagtc agcagtgtcc agtaatgacc tacgcttttg atgtgaactt gctcatccgg
      781 agagtgtgga ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt
      841 tttaaacaga ccacattcca ggcccgcgtg gataatctgg atgttcggcg tcttgttgaa
      901 caggcgctga taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca
      961 gccaggatat gcgcctttcg cttcggtttt cgcgccagcc agtttaccca gcgaatccag
     1021 catgctcacc acgtagtctt taccgctgtc gatcagtgaa cggatcaggc agtgaatttc
     1081 tacgttattg tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt
     1141 tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt
     1201 atcgcgagat ttcgcaatca gagcagcttt atcgttcgct acagagtcca gcaacaaggc
     1261 cagattcttc tctttctctg ccagctcgtt tttcaggatg tcctgatagg tattcaccag
     1321 agatttcagg gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg
     1381 gatggcgtta cgcagtgtgc caccgttgaa atcgataagg cgcaggtcca gctcttccgc
     1441 atgacccgcc aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtgaatttc
     1501 accgccggag tgaccgcctt ttagaccttt taaggttaac ttgaaggttt caaagccagc
     1561 tggaaccgct tcacgatcca aatgcaggtt ggaggtgaag tcgatacccc ccgcgcaacc
     1621 catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca
     1681 gttgctctgt aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac
     1741 ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagcgcag acgccatacc
     1801 aatgccgtta tctgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata
     1861 aggctggata ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat
     1921 atcgaggtgg gcctgcaaga cgaccggttt acgattttcc atacctgcgg tggcaggttt
     1981 acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca
     2041 accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat
     2101 tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag
     2161 tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg
     2221 tcaacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     2281 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     2341 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     2401 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     2461 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     2521 gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     2581 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     2641 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     2701 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     2761 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
     2821 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     2881 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
     2941 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     3001 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     3061 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
     3121 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
     3181 attgtgatga gcgatgctga acgtaccgac gatgatttat acgatactgt gattggctac
     3241 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
     3301 tgtgcggaag aagatccgca actcagtggt cgtcactggc tgcatgcggc tacgttgtat
     3361 aacattgctg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggctttg
     3421 tcaaaccgcg cctatgaaga ggccgctcag cgtctgccgg gcacgatgcg gcagatggag
     3481 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
     3541 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
     3601 ctgtacgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccgtcg
     3661 gtgggctttt cttcaaaatg gaagctgacc caggactcca gcctgttgca tcagcacgtc
     3721 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
     3781 cgtttcggcg ctaacgttgc cgtgcgtctg gcataccttg aatcgccgcg tctgaaagcg
     3841 gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag
     3901 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac
     3961 gaagcgttgc gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctgggacgt
     4021 cgctgcccaa cgccaatgtt atcgggctac tggaagaacg atccgttcag cccggaagag
     4081 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
     4141 ccggtgtatc ggaattttga caaaggtctt caggaaatca cagactggat cgaaaaacgt
     4201 ttgtgttaaa aatttgctaa aatttgccaa tttggtaaaa cagttgcatc acaacaggag
     4261 attgcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca
     4321 ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg
     4381 gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg agttctgggg ctggtggatg
     4441 gagcttgaag cgcaggaatc ccgttttacc tacagttacc agtttggtct gttcgataaa
     4501 gcaggcgact ggaagagtgt tccggtaaaa gacactgaag tggttgaacg actggagcac
     4561 accctacgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg
     4621 gaaccggcgg atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt gaaatgtgcc
     4681 ggatgtatca catccggcaa tatccattaa aactgatagg tcatgccaac cgcgacaata
     4741 tcatcattat taatatt
//
LOCUS       DAZFZC010000463         4552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.213, whole genome shotgun sequence.
ACCESSION   DAZFZC010000463 DAZFZC010000000
VERSION     DAZFZC010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.213"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..595
                     /gene="aadB"
                     /locus_tag="ACHKXP_005655"
     CDS             62..595
                     /gene="aadB"
                     /locus_tag="ACHKXP_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HFO7650394.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     gene            653..1482
                     /gene="aadA1"
                     /locus_tag="ACHKXP_005656"
                     /pseudo
     CDS             653..1482
                     /gene="aadA1"
                     /locus_tag="ACHKXP_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(1724..2767)
                     /locus_tag="ACHKXP_005657"
     CDS             complement(1724..2767)
                     /locus_tag="ACHKXP_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO7650395.1"
                     /translation="MKRIAVDLAKSVYQVAESVRSGQVVQRKRLNREAFRRYIQEQAE
                     PVEWVMEACGTAHYWGRVAQALGHQVRLLHPRYVRPYRRRNKTDRNDCDAMLEATRCT
                     DIHPVPVKSHDQQQLQQLHGLRETWKKSRTQRINLLRGLLREAGIEAPASTAAFIRAA
                     SERVDQPELAPLSRLLHIVLAEINLYEQCMAECEQQLKRWHADDDIVRKLDEVSGIGL
                     LTASALTAAVGRPERFASGRQLSAWLGMTPREFSSGERRKLGHISRQGNVYVRTLLIH
                     GSRAALLAAQRCQARTPEKLTQLQRWAVATAARIGHNKAAVALANKLVRICWAVWCHE
                     RRFNGNWQSTKPA"
     gene            2988..3335
                     /locus_tag="ACHKXP_005658"
     CDS             2988..3335
                     /locus_tag="ACHKXP_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO7650396.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            3329..4168
                     /gene="sul1"
                     /locus_tag="ACHKXP_005659"
     CDS             3329..4168
                     /gene="sul1"
                     /locus_tag="ACHKXP_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO7650397.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            4296..4529
                     /locus_tag="ACHKXP_005660"
     CDS             4296..4529
                     /locus_tag="ACHKXP_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7650398.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEC"
BASE COUNT          899 a         1291 c         1359 g         1003 t
ORIGIN      
        1 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggataggccg
       61 catggacaca acgcaggtca cattgataca caaaattcta gctgcggcag atgagcgaaa
      121 tctgccgctc tggatcggtg ggggctgggc gatcgatgca cggctagggc gtgtaacacg
      181 caagcacgat gatattgatc tgacgtttcc cggcgagagg cgcggcgagc tcgaggcaat
      241 agttgaaatg ctcggcgggc gcgtcatgga ggagttggac tatggattct tagcggagat
      301 cggggatgag ttacttgact gcgaacctgc ttggtgggca gacgaagcgt atgaaatcgc
      361 ggaggctccg cagggctcgt gcccagaggc ggctgagggc gtcatcgccg ggcggccagt
      421 ccgttgtaac agctgggagg cgatcatctg ggattacttt tactatgccg atgaagtacc
      481 accagtggac tggcctacaa agcacataga gtcctacagg ctcgcatgca cctcactcgg
      541 ggcggaaaag gttgaggtct tgcgtgccgc tttcaggtcg cgatatgcgg cctaacaatt
      601 cgtccaagcc gacgccgctt cgcggcgcgg cttaactcag gtgttaaaca tcatgaggga
      661 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      721 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      781 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      841 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      901 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
      961 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     1021 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1081 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     1141 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     1201 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1261 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1321 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcccgcgc
     1381 agatcagttg gaagaatttg ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa
     1441 ataatgtcta acaattcgta tatggactct ccccacaagt agtgagcaaa gcattgcttt
     1501 tgcacctgtc gtcagcgcgg ttgcattcgt atatccggcc tgttgtgggc ttttccgccc
     1561 tggccaatct gtagttcgcg cagcgggggc caagcgttca atcgatcacg cggatcatga
     1621 ttgttatcgg ccttattccg ctgcaggact cgcctgttcc gacagtgctg ctgttcacca
     1681 caaccacctg aaaaccatct tgccgttctg attactcccg ccgtcaggcg ggctttgtgc
     1741 tttgccaatt gccattgaag cgccgctcat ggcaccacac ggcccagcag atccgcacca
     1801 gtttgttggc cagggctacc gctgccttgt tgtggccgat gcgcgccgcc gttgccacgg
     1861 cccagcgttg cagttgcgtc agcttttccg gggtgcgtgc ctgacagcgt tgtgcagcca
     1921 gcaaggctgc ccgtgagcca tggattagca gggtacgcac atagacgttg ccctgtcggc
     1981 tgatgtggcc gagcttgcgg cgctcgccac tgctgaactc gcgcggggtc atgcccagcc
     2041 aggcactgag ctggcgacca ctggcaaagc gctcgggcct gccaacggcg gctgtcaggg
     2101 cgctggcggt cagcagccca atgccgctga cctcatccag cttgcgtacg atgtcatcgt
     2161 cggcatgcca gcgcttgagc tgctgctcgc actcggccat gcactgttca tacaggttga
     2221 tttcggccag gacgatatgc agcagacggc tcaggggtgc gagctcgggc tggtcaaccc
     2281 gctcgctggc ggcgcggatg aaggcagcgg tcgaggcggg ggcctcgata ccagcttcac
     2341 gcaagaggcc gcgcagcagg ttgatccgct gggtgcggct tttcttccag gtttcccgca
     2401 gcccgtgcaa ctgttgcagc tgttgctggt cgtggctttt caccggcacg ggatgaatat
     2461 cggtgcagcg cgtcgcttcg agcatggcgt cgcagtcatt gcgatcggtc ttgttgcggc
     2521 ggcgataggg ccgcacgtag cgcggatgca gcagcctcac ctgatgcccc agggcttgcg
     2581 ctacacgtcc ccagtagtgg gccgtgccac aggcctccat cacccactcg accggttcgg
     2641 cctgctcctg tatatatcgc cggaacgcct ctcggttcag ccgcttgcgc tgcaccacct
     2701 ggccggaacg gacactctcg gcaacttggt aaacggactt ggccagatca actgcaatgc
     2761 gtttcatatc gcctctccca atttcagcca ccacgatgcc tggtatataa gggtggtgcg
     2821 ggggagtcca ttacagcatt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     2881 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     2941 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc
     3001 tttttcttgt tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg
     3061 agggctttac taagcttgcc ccttccgccg ttgtcataat cggttatggc atcgcatttt
     3121 attttctttc tctggttctg aaatccatcc ctgtcggtgt tgcttatgca gtctggtcgg
     3181 gactcggcgt cgtcataatt acagccattg cctggttgct tcatgggcaa aagcttgatg
     3241 cgtggggctt tgtaggtatg gggctcataa ttgctgcctt tttgctcgcc cgatccccat
     3301 cgtggaagtc gctgcggagg ccgacgccat ggtgacggtg ttcggcattc tgaatctcac
     3361 cgaggactcc ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc
     3421 gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc
     3481 ggacgcgagg cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc
     3541 cctgtccgat cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta
     3601 tgcgctcaag cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct
     3661 ctatcccgat attgctgagg cggactgcag gctggtggtt atgcactcag cgcagcggga
     3721 tggcatcgcc acccgcaccg gtcaccttcg acccgaagac gcgctcgacg agattgtgcg
     3781 gttcttcgag gcgcgggttt ccgccttgcg acggagcggg gtcgctgccg accggctcat
     3841 cctcgatccg gggatgggat ttttcttgag ccccgcaccg gaaacatcgc tgcacgtgct
     3901 gtcgaacctt caaaagctga agtcggcgtt ggggcttccg ctattggtct cggtgtcgcg
     3961 gaaatccttc ttgggcgcca ccgttggcct tcctgtaaag gatctgggtc cagcgagcct
     4021 tgcggcggaa cttcacgcga tcggcaatgg cgctgactac gtccgcaccc acgcgcctgg
     4081 agatctgcga agcgcaatca ccttctcgga aaccctcgcg aaatttcgca gtcgcgacgc
     4141 cagagaccga gggttagatc atgcctagca ttcaccttcc ggccgcccgc tagcggaccc
     4201 tggtcaggtt ccgcgaaggt gggcgcagac atgctgggct cgtcaggatc aaactgcact
     4261 atgaggcggc ggttcatacc gcgccagggg agcgaatgga cagcgaggag cctccgaacg
     4321 ttcgggtcgc ctgctcgggt gatatcgacg aggttgtgcg gctgatgcac gacgctgcgg
     4381 cgtggatgtc cgccaaggga acgcccgcct gggacgtcgc gcggatcgac cggacattcg
     4441 cggagacctt cgtcctgaga tccgagctcc tagtcgcgag ttgcagcgac ggcatcgtcg
     4501 gctgttgcac cttgtcggcc gagtgttgat ttgcacccaa atttgacccg gg
//
LOCUS       DAZFZC010000464         3782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.228, whole genome shotgun sequence.
ACCESSION   DAZFZC010000464 DAZFZC010000000
VERSION     DAZFZC010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.228"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            65..307
                     /locus_tag="ACHKXP_005661"
     CDS             65..307
                     /locus_tag="ACHKXP_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO7650399.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(339..989)
                     /gene="tetR(A)"
                     /locus_tag="ACHKXP_005662"
     CDS             complement(339..989)
                     /gene="tetR(A)"
                     /locus_tag="ACHKXP_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO7650400.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1095..2294
                     /gene="tet(A)"
                     /locus_tag="ACHKXP_005663"
     CDS             1095..2294
                     /gene="tet(A)"
                     /locus_tag="ACHKXP_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO7650401.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2326..3210)
                     /locus_tag="ACHKXP_005664"
     CDS             complement(2326..3210)
                     /locus_tag="ACHKXP_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO7650402.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(3348..>3782)
                     /locus_tag="ACHKXP_005665"
     CDS             complement(3348..>3782)
                     /locus_tag="ACHKXP_005665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFO7650403.1"
                     /translation="QGLHGCQHALRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSG
                     RFRTGLPVEGLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLS
                     SVAWASDADYDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT          719 a         1190 c         1169 g          704 t
ORIGIN      
        1 accttagcgt acgatttttt ccgaattctg cgggctcccc tatctcatct gcgcaaggca
       61 gaacgtgaag acggccgccc tggacctcgc ccgcgagcgc caggcgcacg aggccggcgc
      121 gcggacccgc gccacggccc acgagcggac gccgcagcag gagcgccaga aggccgccag
      181 agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa agcccgtagc
      241 gggctgctac gggcgtctga cgcggtggaa agggggaggg gatgttgtct acatggctct
      301 gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt
      361 ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt
      421 ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac
      481 agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg
      541 gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg
      601 gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc
      661 atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc
      721 agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct
      781 ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc
      841 gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc
      901 agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg
      961 atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata
     1021 tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg
     1081 tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc actgtcgcgc
     1141 tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg
     1201 ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttgatgc
     1261 aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct
     1321 tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg gcgcctttcc
     1381 tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag
     1441 ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca
     1501 tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg
     1561 gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga
     1621 cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg
     1681 ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga
     1741 tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt
     1801 tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg
     1861 gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg
     1921 aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct
     1981 tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg
     2041 gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc
     2101 tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca
     2161 cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg
     2221 ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac
     2281 gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag gcgacttccg
     2341 gcaagctata cgcgccctag gagtgcggtt ggaacgttgg cccagccaga tactcccgat
     2401 cacgagcagg acgccgatga tttgaagcgc actcagcgtc tgatccaaga acaaccatcc
     2461 tagcaacacg gcggtccccg ggctgagaaa gcccagtaag gaaacaactg taggttcgag
     2521 tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca
     2581 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc
     2641 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg
     2701 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc
     2761 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc
     2821 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag
     2881 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac
     2941 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag
     3001 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat
     3061 cacgagcaat aaacccgccg gcaacgcccg cagcatcgcg accgtcatcg gtgagaagtt
     3121 cggcaggtat tgggtggtga caatgtaggt gctgccccaa atggcaggtg ctatcgctgt
     3181 gaacaataaa tcgggcgtgc gtaatgacat gcgattacaa gacctccgct acggcgatgg
     3241 gtttttgaga gttgcaggcg ccgcgatgac cgcaccctga cttggactgg ccctgcctcg
     3301 caccaatcca cctgcggcta tggccgcgat catgcgaggc ccgaaaatta cacgacctgt
     3361 acacgtccgc cgaacccgga acgcaacaga gcttcgtggg catcccgatc cgggtcgtag
     3421 cagcagtcct ggaccaaacg cacgtcgtag tccgcatcac tagcccaggc gacgcttgaa
     3481 agaacaacgc cggtggtgct tatcccggcc atgacaagcg tgcttacgcc gcgcgtccga
     3541 aggtcggcgt cgagcgccgt gccataaaag acgctggctc gcggacatgc atagaaaagg
     3601 tcgccccgtt cgatcgcaag cccttcgacg ggcagaccgg tgcgaaatcg tccgctcggg
     3661 agatacggtg agatttggcg attcgtcgcc ggcggcgcat gttcatactc ttcgccgagc
     3721 gaaaagttgg ggaacagcac gggccggccg cgcaaggcgt gctggcagcc gtgcagaccc
     3781 tg
//
LOCUS       DAZFZC010000465         3007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.245, whole genome shotgun sequence.
ACCESSION   DAZFZC010000465 DAZFZC010000000
VERSION     DAZFZC010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.245"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..245
                     /locus_tag="ACHKXP_005666"
     CDS             <1..245
                     /locus_tag="ACHKXP_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO7650404.1"
                     /translation="RYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYL
                     SPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
     gene            247..969
                     /locus_tag="ACHKXP_005667"
     CDS             247..969
                     /locus_tag="ACHKXP_005667"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HFO7650405.1"
                     /translation="MLYFPFSETTPSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVER
                     IDADQWRIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGET
                     ADLAPTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSD
                     MALSKGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGRE
                     VMGIVQGGSVSWQLGRQRGLER"
     gene            1186..2400
                     /gene="floR"
                     /locus_tag="ACHKXP_005668"
     CDS             1186..2400
                     /gene="floR"
                     /locus_tag="ACHKXP_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HFO7650406.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSILAFVPALGPIAGA
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTFFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            2428..2733
                     /locus_tag="ACHKXP_005669"
     CDS             2428..2733
                     /locus_tag="ACHKXP_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7650407.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            2845..>3007
                     /locus_tag="ACHKXP_005670"
     CDS             2845..>3007
                     /locus_tag="ACHKXP_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO7650408.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYV"
BASE COUNT          544 a          875 c          922 g          666 t
ORIGIN      
        1 agcgctaccg ggccagcggc ctcaacctgg tgacggcggc catcgtgctg tggaacacgg
       61 tctacctgga gcgtgcggcg catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc
      121 tattgcagta cctgtcgcca ctcggctggg agcacatcaa cctgaccggt gattacctat
      181 ggcgcagcag cgccaagatc ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg
      241 cttagcgtgc tttattttcc gttttctgag acgacccctt cctatacgtg ccgcgttcca
      301 ggcggcgact atgagggcta tgtcgatgcc catgtgcgcc ggctggaggc gctacgccgg
      361 gccggtatcg tcgagcggat cgacgccgac caatggcgca tccccgatga tctggtcagc
      421 cgtgccgccg cccatgacgc cggccgagac agtcaggcca gcgttcgcgt cctttccccg
      481 gtcgatctga acaaacagat cggatcggac ggcgcgacct ggctggaccg gcggctgatc
      541 cacggcgaga cggccgacct tgcgccaacc ggcttcgggc aacaagtccg cgaagccatg
      601 gaccagcgcc gcgagcacca tatcgaacag ggcgacgcca cccgcagccg ggacagccgc
      661 gtcttctacc ggcgcaacct tctcgccatc ctgcgggagc gcgaggtagc cggcgtcgga
      721 tcggatatgg ctttgagtaa gggcctgccg ttccgcgccg ccacggacgg cgagagcgtc
      781 agcggcaagt ttaccggaac cgtgcatcta tcgagcggca agttcgccgt ggtcgagaaa
      841 tcccatgagt tcacccttgt cccgtggcgg ccgatcatcg accgccaact cggccgcgag
      901 gttatgggca tcgtgcaggg cgggtcggtg tcgtggcagt tagggcggca gagggggctg
      961 gaacgctgag tgcgcccatg ccgcattgcg aagcaaaaga taatcggata aaatgtagca
     1021 attcatattc gtaagcgtgg agtaatcaga tgggaaattc caagtcagca gacaagtaag
     1081 ccgcaacaac cagtattgtt gttgcggcgc tctgtaaggc tagtctcatc tgattgctga
     1141 cgagcagacg tcgcccggta ttccttaatc gagggttgat tcgtcatgac caccacacgc
     1201 cccgcgtggg cctatacgct gccggcagca ctgctgctga tggctccttt cgacatcctc
     1261 gcttcactgg cgatggatat ttatctccct gtcgttccag cgatgcccgg catcctgaac
     1321 acgacgcccg ctatgatcca actcacgttg agcctctata tggtgatgct cggcgtgggc
     1381 caggtgattt ttggtccgct ctcagacaga atcgggcgac ggccaattct acttgcgggc
     1441 gcaacggctt tcgtcattgc gtctctggga gcagcttggt cttcaactgc accggccttt
     1501 gtcgctttcc gtctacttca agcagtgggc gcgtcggcca tgctggtggc gacgttcgcg
     1561 acggttcgcg acgtttatgc caaccgtcct gagggtgtcg tcatctacgg ccttttcagt
     1621 tcgatactgg cgttcgtgcc tgcgctcggc cctatcgccg gagcattgat cggcgagttc
     1681 ttgggatggc aggcgatatt cattactttg gctatactgg cgatgctcgc actcctaaat
     1741 gcgggtttca ggtggcacga aacccgccct ctggatcaag tcaagacgcg ccgatctgtc
     1801 ttgccgatct tcgcgagtcc ggctttttgg gtttacactg tcggctttag cgccggtatg
     1861 ggcaccttct tcgtcttctt ctcgacggct ccccgtgtgc tcataggcca agcggaatat
     1921 tccgagatcg gattcagctt tgccttcgcc actgtcgcgc ttgtaatgat cgtgacaacc
     1981 cgtttcgcga agtcctttgt cgccagatgg ggcatcgcag gatgcgtggc gcgtgggatg
     2041 gcgttgcttg tttgcggagc ggtcctgttg gggatcggcg aactttacgg ctcgccgtca
     2101 ttcctcacct tcatcctacc gatgtgggtt gtcgcggtcg gtattgtctt cacggtgtcc
     2161 gttaccgcga acggcgcttt ggcagagttc gacgacatcg cgggatcagc ggtcgcgttc
     2221 tacttctgcg ttcaaagcct gatagtcagc attgtcggga cattggcggt ggcactttta
     2281 aacggtgaca cagcgtggcc cgtgatctgt tacgccacgg cgatggcggt actggtttcg
     2341 ttggggctgg tgctccttcg gctccgtggg gctgccaccg agaagtcgcc agtcgtctaa
     2401 ccgacgactg gtagcaggcc cgctccgatg cggcgcacta accatcgaaa cctcgtgaat
     2461 gtcggtatcc tgtctggcag gataccgctc atttcccttg ttcagttcat cgccgtcgcc
     2521 gagcatctga attttcggca tgcagccaag gcacttggta tcagccagtc gagcgtcagc
     2581 gcgcgtgtga aagcgctgga ggataacctt ggtgtcctgc tatttgagcg ccatgcgcgg
     2641 ggcgttcggc taacagacgc aggcaggcac ttcatggagc gtgtcacggc gggtgtcgat
     2701 caactcgatc acgcagtgaa gaccgcggag tgacgggcac tggctggcaa tgtctagcaa
     2761 cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa
     2821 cacagtattg caaggacgcg gaacatgcct catgtggcgg ccaggacggc cagccgggat
     2881 cgggatactg gtcgttacca gagccaccga cccgagcaaa cccttctcta tcagatcgtt
     2941 gacgagtatt acccggcatt cgctgcgctt atggcagagc agggaaagga attgccgggc
     3001 tatgtgc
//
LOCUS       DAZFZC010000466         1717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.295, whole genome shotgun sequence.
ACCESSION   DAZFZC010000466 DAZFZC010000000
VERSION     DAZFZC010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.295"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..150
                     /locus_tag="ACHKXP_005671"
     tRNA            74..150
                     /locus_tag="ACHKXP_005671"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:108..110,aa:Arg,seq:tct)
     gene            241..1200
                     /locus_tag="ACHKXP_005672"
     CDS             241..1200
                     /locus_tag="ACHKXP_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO7650409.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1212..1481
                     /locus_tag="ACHKXP_005673"
     CDS             1212..1481
                     /locus_tag="ACHKXP_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO7650410.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          449 a          359 c          435 g          474 t
ORIGIN      
        1 ggacagagcg ccagccttcg aagctggctg cgcggggttc gagtcctcga tggcggtcca
       61 ttatctgcat tatgcgttgt tagctcagcc ggacagagca attgccttct gagcaatcgg
      121 tcactggttc gaatccagta caacgcgcca tatttattta ccaggctcgc ttttgcgggc
      181 cttttttata tctgcgccgg gtctggtgct gattacttca gccaaaagga acacctgtat
      241 atgaagtgta tattatttaa atgggtactg tgcctgttac tgggtttttc ttcggtatcc
      301 tattcccggg agtttacgat agacttttcg acccaacaaa gttatgtctc ttcgttaaat
      361 agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg
      421 tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat
      481 gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg
      541 gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt tacacatata
      601 tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa
      661 cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca
      721 tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt
      781 ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt
      841 cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc
      901 actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc
      961 agagtgggga gaatatcctt taataatata tcagcgatac tggggactgt ggccgttata
     1021 ctgaattgcc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga gagtcaacca
     1081 gaatgtcaga taactggcga caggcctgtt ataaaaataa acaatacatt atgggaaagt
     1141 aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa
     1201 aggagttaag catgaagaag atgtttatgg cggttttatt tgcattagct tctgttaatg
     1261 caatggcggc ggattgtgct aaaggtaaaa ttgagttttc caagtataat gaggatgaca
     1321 catttacagt gaaggttgac gggaaagaat actggaccag tcgctggaat ctgcaaccgt
     1381 tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt acctgtgaat
     1441 caggctccgg atttgctgaa gtgcagttta ataatgactg aggcataacc tgattcgtgg
     1501 tatgtgggta acaagtgtaa tctgtgtcac aattcagtca gttgacagtt gcctgtcaga
     1561 ctgagcattt gttaaaaaaa tttcgcatgg tgaatccccc tgtgtggagg ggcgactggt
     1621 gaaaaatcct tgcttgtgat tcattatcga cacgggttcg gtggtaccag gccgaactca
     1681 ccgggaggca cccggcacca tgcaatggca catagcg
//
LOCUS       DAZFZC010000467         1698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.298, whole genome shotgun sequence.
ACCESSION   DAZFZC010000467 DAZFZC010000000
VERSION     DAZFZC010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.298"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..815
                     /locus_tag="ACHKXP_005674"
                     /note="aph(3'')-Ib"
     CDS             12..815
                     /locus_tag="ACHKXP_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO7650411.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            815..1651
                     /locus_tag="ACHKXP_005675"
     CDS             815..1651
                     /locus_tag="ACHKXP_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO7650412.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          345 a          447 c          501 g          405 t
ORIGIN      
        1 aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct gactggttgc
       61 ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg catgccttcg
      121 cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac cgcctcattt
      181 ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag gaacaggagg
      241 gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg tctggagcgg
      301 atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac agcctatcgg
      361 ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg
      421 tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt acgccgcagc
      481 tcgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg
      541 atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac cctaaaactc
      601 ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc tatgccgatt
      661 tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa gcagagcgcg
      721 ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa cgccttgcct
      781 tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg tttttcctgc
      841 tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc tcgtttggaa
      901 agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta tagaagacat
      961 tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg gagcagtccg
     1021 gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc gaatgctctc
     1081 tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg aactaatggc
     1141 gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga tccgggatcg
     1201 ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc aaactgacta
     1261 cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac tgcgtgggct
     1321 acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc tggtgataga
     1381 tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct acgatccggc
     1441 tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg acgcattctc
     1501 tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt atgggtgcct
     1561 ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta tcgcggccgc
     1621 gatcaagcag gtgcgacaga cgtcatacta gatatcaagg gcactgttgc aaagttagcg
     1681 atgaggcagc cttttgtc
//
LOCUS       DAZFZC010000468         1673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.300, whole genome shotgun sequence.
ACCESSION   DAZFZC010000468 DAZFZC010000000
VERSION     DAZFZC010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.300"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..588
                     /gene="dfrA36"
                     /locus_tag="ACHKXP_005676"
     CDS             <1..588
                     /gene="dfrA36"
                     /locus_tag="ACHKXP_005676"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA36"
                     /protein_id="HFO7650413.1"
                     /translation="LLSKSDILLQFIYFYNTFIFLIAFFMKVSLIVAMDLEKGIGKNN
                     DLMWHLPADMLFFKETTLNHIVVMGRKNFESIPERFRPLPNRENAILTRNTAFEAPNC
                     TVFHSMEGCLKHYENEDKRTVFIIGGGQIYEEALEKNRVDEMFITFVDHTFGADTFFP
                     SIDFSLWNEEVLRVHEADSKNAYNFTVKKFTKKLS"
     gene            980..1426
                     /locus_tag="ACHKXP_005677"
     CDS             980..1426
                     /locus_tag="ACHKXP_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFO7650414.1"
                     /translation="MFTKACEYGIRACIFVALNSMEKRRVSLKEIAQSIDSPEYFTAK
                     ILQKLAKGKIIYSVQGAQGGFEMEEAKIRTISIEDIVLVIDGEVAFTMCVLGLKKCSE
                     VNPCPVHHKYKNIKADLKEMIKTTTVFDMVDQVNDGHSFLKIFELE"
     gene            1450..1635
                     /locus_tag="ACHKXP_005678"
     CDS             1450..1635
                     /locus_tag="ACHKXP_005678"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HFO7650415.1"
                     /translation="MDTIRITKCFTFDMAHALKGYDGLCRNIHGHTYMLRVTLAGKIK
                     HEDSNPKNGFVLDFGDV"
BASE COUNT          539 a          283 c          311 g          540 t
ORIGIN      
        1 ttgctttcaa aaagtgatat attgcttcaa tttatatact tttataatac attcattttt
       61 ctaatagcgt ttttcatgaa agtaagtttg atagttgcaa tggatcttga aaagggcatt
      121 ggtaaaaaca acgatttgat gtggcattta ccggccgata tgcttttttt taaagaaact
      181 acactgaatc acattgtggt tatgggtagg aaaaatttcg aatcaatccc tgaaaggttt
      241 cgtccacttc caaatcggga aaatgcaata ttaactcgga atacagcttt tgaagcaccg
      301 aattgtactg tttttcacag catggaaggt tgcttgaaac actatgagaa cgaagataag
      361 agaaccgttt ttatcattgg tggcggacaa atatatgagg aggctttaga aaaaaacagg
      421 gttgatgaaa tgtttataac ctttgtggat catacttttg gtgcggatac attttttcct
      481 tccatcgatt tttcgctttg gaatgaagag gtgctgcgtg tgcatgaagc agattctaaa
      541 aatgcgtata attttacggt caaaaaattc actaagaagt tatcctgatt cgctttttcg
      601 ggttaacgtt attttgaggt gctggcacta aaacattttt gctattcttc taaggtggaa
      661 aaatctaaat ttcacgaacc tttattttgt cgaagtgctt cttttatcgt agcgttcact
      721 ttgatataca gcctttaatt ctgtaacttg tttaaataat aaagaagccc tgtttttctg
      781 atatacatgc tgttctgaac caaatcaact tcaatagaag gaatgcctgt tgtcttttcg
      841 atcaaactga taaatcatct tttttctcaa atccaagatc ttaaaataca ctgtttttac
      901 aatcaatttc aattttaaat tagactaatc tatccacttt gaaattactt cactactttt
      961 accgaaaatt acgaaaacaa tgtttacaaa agcatgtgag tatggcattc gtgcctgtat
     1021 ttttgtagcg cttaattcga tggaaaaaag gcgtgtcagt ttaaaagaaa ttgcacagtc
     1081 tattgattca cccgaatatt tcaccgcaaa gattcttcag aaactcgcca agggaaaaat
     1141 aatctattcc gttcaaggag cacagggagg ttttgagatg gaggaggcga agattagaac
     1201 gatttccatt gaagatattg ttttggtcat tgatggtgag gtagccttta cgatgtgcgt
     1261 tcttggtttg aaaaaatgtt cagaagtaaa cccttgccct gttcatcata aatacaaaaa
     1321 catcaaagct gatctcaaag aaatgatcaa aaccaccacg gttttcgaca tggtagatca
     1381 agtaaacgac ggacattctt ttctgaaaat ttttgaatta gaataaagta acagctaaca
     1441 atcaaattcg tggatacaat cagaattacc aagtgcttca ccttcgacat ggcgcacgca
     1501 ttgaaaggat acgacggtct ttgccgaaac attcatggac atacttatat gttaagagtc
     1561 actttagctg gcaaaattaa acacgaggac agcaacccta aaaatggttt cgtattggat
     1621 tttggagacg tataggaaga ataaacgccc ttttcaccca agtccaacag ctt
//
LOCUS       DAZFZC010000469         1608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.305, whole genome shotgun sequence.
ACCESSION   DAZFZC010000469 DAZFZC010000000
VERSION     DAZFZC010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.305"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..379
                     /locus_tag="ACHKXP_005679"
     CDS             <1..379
                     /locus_tag="ACHKXP_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7650416.1"
                     /translation="MISLSPPTICNSAADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGAT
                     EIAHQLSIARSTVYKILEDERAS"
     gene            562..1422
                     /locus_tag="ACHKXP_005680"
     CDS             562..1422
                     /locus_tag="ACHKXP_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO7650417.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          435 a          354 c          403 g          416 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc gccgatatga tccaactgat
       61 aaaggaattt gacgctcagg gcgtggcagt ccggttcatt gatgacggga tcagtaccga
      121 cggtgatatg gggcaaatgg tggtcaccat cctgtcggct gtggcacagg ctgaacgccg
      181 gaggatccta gaacgcacga atgagggccg acaggaagca aagctgaaag gaatcaaatt
      241 tggccgcagg cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga agggcactgg
      301 tgcaacggaa attgctcatc agctcagtat tgcccgctcc acggtttata aaattcttga
      361 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      421 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctggta aatgcttcaa
      541 taatattgaa aaaggaagag tatgagtatt caacattttc gtgtcgccct tattcccttt
      601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      781 ctatgtggtg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
      841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgctgcc
      961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1081 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     1141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1441 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1501 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1561 tcagacccct tatggcagag cagggaaagg aattgccggg ctatgtgc
//
LOCUS       DAZFZC010000470         1119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.345, whole genome shotgun sequence.
ACCESSION   DAZFZC010000470 DAZFZC010000000
VERSION     DAZFZC010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.345"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..942
                     /locus_tag="ACHKXP_005681"
     CDS             127..942
                     /locus_tag="ACHKXP_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HFO7650418.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          298 a          231 c          258 g          332 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccc tctgatgtta cattgcacaa
       61 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
      121 ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
      181 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      241 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      301 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
      361 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
      421 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
      481 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
      541 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
      601 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
      661 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
      721 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
      781 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
      841 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
      901 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
      961 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcttccct accctcatgg
     1021 ccgcctccca ggaatcgacc caggtgctta tcgtggacat aatcctttac tcataaattg
     1081 gcactgttgc aaagttagcg atgaggcagc cttttgtct
//
LOCUS       DAZFZC010000471          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.376, whole genome shotgun sequence.
ACCESSION   DAZFZC010000471 DAZFZC010000000
VERSION     DAZFZC010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.376"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005682"
     CDS             25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO7650419.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          156 a          258 c          252 g          190 t
ORIGIN      
        1 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
       61 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
      121 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
      181 aaccccgacg ccgcgcctgt ttcgtccgac acagaaatcg agcgtatcgc gccggtgctg
      241 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
      301 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
      361 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
      421 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
      481 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
      541 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
      601 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
      661 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
      721 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
      781 cccttgcgcg acgggctggc ggtattggcg gcgctaaaag aaaccgcaag aattcgttaa
      841 ctgcacattc gggata
//
LOCUS       DAZFZC010000472          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.377, whole genome shotgun sequence.
ACCESSION   DAZFZC010000472 DAZFZC010000000
VERSION     DAZFZC010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.377"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005683"
     CDS             25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO7650420.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          155 a          258 c          253 g          190 t
ORIGIN      
        1 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
       61 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
      121 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
      181 aaccccgacg ccgcgcctgt ttcgtccgac acagaaatcg agcgtatcgc gccggtgctg
      241 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
      301 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
      361 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
      421 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
      481 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
      541 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
      601 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
      661 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
      721 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
      781 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
      841 ctgcacattc gggata
//
LOCUS       DAZFZC010000473          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.378, whole genome shotgun sequence.
ACCESSION   DAZFZC010000473 DAZFZC010000000
VERSION     DAZFZC010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.378"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005684"
     CDS             25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO7650421.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          155 a          258 c          252 g          191 t
ORIGIN      
        1 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
       61 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
      121 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
      181 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
      241 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
      301 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
      361 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
      421 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
      481 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
      541 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
      601 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
      661 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
      721 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
      781 cccttgcgcg acgggctggc ggtattggcg gcgctaaaag aaaccgcaag aattcgttaa
      841 ctgcacattc gggata
//
LOCUS       DAZFZC010000474          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.379, whole genome shotgun sequence.
ACCESSION   DAZFZC010000474 DAZFZC010000000
VERSION     DAZFZC010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.379"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005685"
     CDS             25..840
                     /gene="sul2"
                     /locus_tag="ACHKXP_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO7650422.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          154 a          258 c          253 g          191 t
ORIGIN      
        1 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
       61 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
      121 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
      181 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
      241 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
      301 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
      361 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
      421 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
      481 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
      541 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
      601 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
      661 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
      721 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
      781 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
      841 ctgcacattc gggata
//
LOCUS       DAZFZC010000475          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2308W43314
            SAMN43283855-rid23078613.guided.404, whole genome shotgun sequence.
ACCESSION   DAZFZC010000475 DAZFZC010000000
VERSION     DAZFZC010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283855
            Sequence Read Archive: SRR30325193
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351455.1
            Genome Coverage       :: 92x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:20:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,686
            CDSs (total)                      :: 5,587
            Genes (coding)                    :: 5,330
            CDSs (with protein)               :: 5,330
            Genes (RNA)                       :: 99
            rRNAs                             :: 3, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 117 of 257
            Pseudo Genes (incomplete)         :: 149 of 257
            Pseudo Genes (internal stop)      :: 46 of 257
            Pseudo Genes (multiple problems)  :: 49 of 257
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283855-rid23078613.guided.404"
                     /serotype="O 80:H 2"
                     /isolate="2308W43314"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..529
                     /gene="dfrA5"
                     /locus_tag="ACHKXP_005686"
     CDS             56..529
                     /gene="dfrA5"
                     /locus_tag="ACHKXP_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA5"
                     /protein_id="HFO7650423.1"
                     /translation="MKVSLMAAKAKNGVIGCGPHIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSAWTADNDNVIVFPSIEEAMYGLAELTDHVIVSGGGEI
                     YRETLPMASTLHISTIDIEPEGDVFFPNIPNTFEVVFEQHFSSNINYCYQIWQKG"
BASE COUNT          187 a          150 c          161 g          169 t
ORIGIN      
        1 gatgttacgc agcagggcag tcgccctaaa acaaagttaa cccggaacca aaattgtgaa
       61 agtatcatta atggctgcaa aagcgaaaaa cggagtgatt ggttgcggtc cacacatacc
      121 ctggtccgcg aaaggagagc agctactctt taaagccttg acgtacaacc agtggctttt
      181 ggtgggccgc aagacgttcg aatctatggg agcactccct aataggaaat acgcggtcgt
      241 tactcgctca gcctggacgg ccgataatga caacgtaata gtattcccgt cgatcgaaga
      301 ggccatgtac gggctggctg aactcaccga tcacgttata gtgtctggtg gcggggagat
      361 ttacagagaa acattgccca tggcctctac gctccatata tcgacgattg atattgagcc
      421 ggaaggagat gttttctttc cgaatattcc caataccttc gaagttgttt ttgagcaaca
      481 ctttagctca aacattaact attgctatca aatttggcaa aagggttaac aaagctatgc
      541 aattgacggt aaaaagcttc gttcgcttcg cttgctacgc ttcttaccgc aattgataac
      601 ggcgttagat gcactaagca cataattggg cactgttgca aagttagcga tgaggcagcc
      661 ttttgtc
//
